LOCUS       DAFNNB010000001        82139 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0001, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000001 DAFNNB010000000
VERSION     DAFNNB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 82139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0001"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(132..698)
                     /locus_tag="L3M60_000001"
     CDS             complement(132..698)
                     /locus_tag="L3M60_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6224712.1"
                     /translation="MATRPIEIDEYKKIMELLHTGFTYSENGVEKRFRKNPKVALALM
                     LEANLGLRISDILRLKIGNFKGNMLEINEKKTGKLQYRPVNKNIIESINEHARKYNLK
                     SNDYLVNIKTKAIQKQLRIICKHLNLYNISTHSFRKLYATTQFEKSNNNLELVKELLN
                     HSSVATTQRYIRVTQQAINEASENFFIG"
     gene            complement(965..2224)
                     /locus_tag="L3M60_000002"
     CDS             complement(965..2224)
                     /locus_tag="L3M60_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HBN6224713.1"
                     /translation="MKKNKIIAFFAPAIIVGVLVLGIILYPNEAIKSAREGFSIWSNV
                     LVPSLLPFIIGANLIVDLKIVDIIGFIINPITRFVFNVSGKSALAFAISTVSGYPVGV
                     SLASEFRSNRQISKHEAQRLVSFCSTSGPLFIIGSVGTGMFQNSNLGYLMILCHYLGT
                     ILVGLFFRNYGNESLPKTKLDLKTNIKNVINIRNSSGNGFFVLFGNAVFNGVNTLLTV
                     GGFVIVFSVVFKILSLFNVISLIASVIYLPLSFLGVSKELCQAFVSGLFEITIGCNKV
                     SSITSAPEILRASLASFLIGFSGLSILAQCCTFLSKTDIDLKLYILSKFAHGILAAIF
                     IFILYPIANSAVLVSSFADTYNVIYNNLIWFYYLSYYDTILQIVIIIYLISAVFIAKK
                     HMKALSTNGKLMRYKNTFFIKKFSRKK"
     gene            2349..3062
                     /locus_tag="L3M60_000003"
     CDS             2349..3062
                     /locus_tag="L3M60_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBN6224714.1"
                     /translation="MFNSIASKKVYEQVIEQIQYKILNGELKKGDKLLSERELSEQMN
                     VSRTSIREAIRVLETMGVIESRQGEGNFICTNIEKTLIEPLSMIFKLNNGTLGDILEL
                     RIILEIEIAKLASKRITSSEAIELKNIIDEMRVETNKKDNNRVLVLLDQKFHSKLATL
                     SKNYLIQSLFMTASKLFDGFIEDAREKIIAEPFNENILLKQHEAIYNAVVENDVELAC
                     EKAKEHMDFISKNYRKNEK"
     gene            3180..4454
                     /gene="larA"
                     /locus_tag="L3M60_000004"
     CDS             3180..4454
                     /gene="larA"
                     /locus_tag="L3M60_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent lactate racemase"
                     /protein_id="HBN6224715.1"
                     /translation="MVKLKVPYSKQGMILEIPDERFLGVLESKSDNYIEHKTQEEIVE
                     ESMNNPIGSNSLEELVKGKKNVVIITSDHTRPVPSRITMPIILRRIRKINPEIDVKII
                     VATGFHRPSTREELIYKMGEEIVDNEDIVMHVSTDDKSMCKVGILPSGGDLYINKLAY
                     EAELLIAEGFIEPHFFAGFSGGRKSILPGIASAKTIMYNHCSDFIDSDNSRTGKLNNN
                     PIHEDMVYAAKVAKLAFILNVVIDKDKKIIASFAGDVEKAHTKGCRFVTELSEVNSIK
                     SDIVVTTNGGYPLDQNIYQSVKGMTAAEATCKDGGVIIMIAACNDGHGGQSFYENVAN
                     ADSPKQLLDKIRSVDRVDTVPDQWEFQILARILSKYTVIMVTDKCNPEMIKKMHMKHA
                     FNFKEALEKATSIVGKDSKVVVIPDGVSVIVK"
     gene            4471..5259
                     /locus_tag="L3M60_000005"
     CDS             4471..5259
                     /locus_tag="L3M60_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBN6224716.1"
                     /translation="MEILTCIKQVPGTTSVEVDETTGVLKRDGVDSKMNPYDLYALET
                     ALRIKQEKKANLKVLSMGPPQAKKVIEESFMMGADEGALISDRRFGGADVLATSYTIS
                     QGIKKMGKVDLIICGKQTTDGDTAQVGPEVAEFLDIPHVTNVTKLIEAKDESIVVEID
                     MPNDLQVCEIEYPCLITVEKDIFQPRLPSFKLKLDTKDREIPVYSLDDFEDKDENNYG
                     LNGSPTQVVRIFPPKPNTDKNIVRGNADELSSALVNKLEELKLV"
     gene            5273..6493
                     /locus_tag="L3M60_000006"
     CDS             5273..6493
                     /locus_tag="L3M60_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HBN6224717.1"
                     /translation="MSKIVVNQDKITDLKRVLEICPFGAIEEKSGVVEISAGCKMCKL
                     CVKSGPKGAFEFVESSKVQINKDEWRGIAVYVEHHNGNIHPVTYELIGKAREMASKIK
                     QPVYCVFVGKDIKDKCNNLISYGVDEVFVYDEDEFKDFRIEPYSKAIENFINKIKPTI
                     VLVGGTTLGRSLAPRLAARFRTGLTADCTILDIQSNTDLDQIRPAFGGNIMAHINTPN
                     NRPQFATVRYKIFSVPEKIENATGKVTLCKIEKKDLSSKIKVLSVKEKNKEVGLEEAE
                     VIVVASRAIKKQEDMEMMYKLANKLNAQVAGTRPVIEAGWIDAKKQIGLSGRTVKPKL
                     IITCGVSGAVQFVAGMQGADYIVAINKDDKAPILDVAHLALIGDIYDIIPKLIEKIEN
                     NKDDNQKYMASAAK"
     gene            6504..7895
                     /locus_tag="L3M60_000007"
     CDS             6504..7895
                     /locus_tag="L3M60_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBN6224718.1"
                     /translation="MYKLVDKKDIDFLIDTCGEENVLVGSDINEDFSHDELGGIEKYP
                     EVLVNVLETEQVSKIMKYAYKNNIPVTPRGQGTGLVGAAVAINGGIMINLCKMNKILE
                     VDYENLTLTVEPGVLLMTIGQYVQDRDLFYPPDPGEKSATIAGNINTNAGGMRAVKYG
                     VTRDYVRGLEVVLPNGEVINVGGKVVKNSSGYSIKDLLVGSEGTLGIVTKAILKLLPL
                     PKKSISLLVPFPDLSMAIETVPKIIKSKSIPTAIEFMERDVILAAEEFLGKKFPDNTS
                     DAYLLLTFDGNSTEDIEKEYEKVANLCLENGALDVFISDTQERNDSIWSARGAFLEAI
                     KASTTQMDECDVVVPRDKIAEFIRYTHELQDKLKIRIKSFGHAGDGNLHIYILKDGMD
                     DNTWKIRLKETFDYMYKKSRELSGQVSGEHGIGYAKKEYLHESNSDAYMMLIKNIKLA
                     FDPKNILNPGKIY"
     gene            complement(8158..9438)
                     /locus_tag="L3M60_000008"
     CDS             complement(8158..9438)
                     /locus_tag="L3M60_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HBN6224719.1"
                     /translation="MNILGLIVEYNPFHNGHIYHLSKSIEKTNATHTVAIMSGNFLQR
                     GEPALFDKYTRAKIAVENGVDLVIELPTLFACQSAEIFSHGAITILNSLNCINSICFG
                     SEEGNTEILYNIAEILANEPTEFKLLLKKYLDDGLLFPTARANALFDYIHQDKKNNFI
                     QNYSKEQLSKILNSSNNILGIEYIKNLIHLNSSIKPFTISRVQSEYNSEEIKNNICSA
                     TAIRTSLKENINFFHLKNVIPEKTYNILNEKIDNGFLPMFDDLFFDTLKTIILRDMDT
                     LKDYFEVNEGIENRIYRDIFTSEFLHELQLSIKSKRYTLTKIKRTLNNILLGIKKSDM
                     QLIKNIDTIPYIRILAFNDKGREVLKEIKNKSEIKIINKFSNISFSLDDTIFKTLISY
                     DIKSTNIYNSVYYKNNKDLVKGPMDFYTHPIYVK"
     gene            9892..11088
                     /locus_tag="L3M60_000009"
     CDS             9892..11088
                     /locus_tag="L3M60_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBN6224720.1"
                     /translation="MKILVLNCGSSSLKYQLIDMNNEEVLCIGLVERIGIEGSILKHE
                     KAGRDDKYVVEQPMKDHKDAIALVLEAVAHPEFGAVKEMKEIDAVGHRVVHAGEKFAT
                     SVVITPEVEDALKECIDLAPLHNPANIMGIDACKAILPDVPMVAVFDTAFHQTMPKSS
                     YLYGLPHELYTKYGVRRYGFHGTSHNYVSQRAAEILGKDIKDLKIVTCHLGNGASIAA
                     VDGGKCVDTSMGFTPLEGLIMGTRCGDIDPAILPFLMRKEGLDADGLDKLMNKESGVY
                     GMTGISSDFRDIEDAAKNGDERAQATLEAYVKKVQKYIGAYAAEMNGLDVVVFTAGVG
                     ENGKAIRADIASNMEFLGMKLDKEANDVRGKETVISTADSKVKMLLIPTNEELMIARD
                     TLRLVK"
     gene            11181..11705
                     /locus_tag="L3M60_000010"
     CDS             11181..11705
                     /locus_tag="L3M60_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HBN6224721.1"
                     /translation="MKVSIEKINRKETDKIDLNFCEKIDTISYCDEIYKLVSPVNLKG
                     KVSKTNKGLYLDIDVNFTIVDNCSRCLKEVEIPLEYSIQGFLVKEEDYDEDEFEEFDP
                     FIFDGEEIDLIDIIEQTLDFNVPHKVLCSENCKGLCQVCGANLNEEECSCSEITNDEE
                     YIDPRFAKLKDLFN"
     gene            11730..11906
                     /gene="rpmF"
                     /locus_tag="L3M60_000011"
     CDS             11730..11906
                     /gene="rpmF"
                     /locus_tag="L3M60_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBN6224722.1"
                     /translation="MAVPKRKTSKSNTKMRRAANSKMEATGFVSCPQCHEPKLPHRVC
                     PDCGYYKGKEVVSK"
     gene            12113..12670
                     /gene="fapR"
                     /locus_tag="L3M60_000012"
     CDS             12113..12670
                     /gene="fapR"
                     /locus_tag="L3M60_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HBN6224723.1"
                     /translation="MKKKSKAQRQKELIDMLKTDPFYTDEELSSLFDVSIQTIRLDRM
                     SLNIPELRERVKSIAETQSSKVKTLGVKEITGEIIDLSVGRLGISMLEVTQDMIYSKT
                     NTLKDTYIFSLADSLAMAIIDAPKVIMRVANVKSFKLIEQQDRLIAKAEVYRNIDKKH
                     YVKVVINNKAQEQIFRGKFIFEELD"
     gene            12681..13703
                     /gene="plsX"
                     /locus_tag="L3M60_000013"
     CDS             12681..13703
                     /gene="plsX"
                     /locus_tag="L3M60_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBN6224724.1"
                     /translation="MKIVIDGMGGDNAPKSNVEGAVNAIKEYQVDLIITGDKDLLEKE
                     FSNYEFDRNKLEIVHTTEIIENEDKPVKAIRSKKDSSMVVSLNLVKEGKADAIISAGN
                     TGALLAGGLFVVGRIKGIDRPCLCSAIPNVKRGMTLIADCGANADCKPKNLVEFAAMS
                     NIYSRKVLGLENPKVALANVGLEEGKGNDLVKRSYEEIKKLDLNFIGNVEAREVINAY
                     TDIIICDGFTGNILLKSAEGVALSVMSLIKETFMASTKSKIGALLIKDDLRKLKSFID
                     YSEYGGAPLLGLNGGVIKAHGSSDAKAIKNAINQGIKFSKGKVVEDIKQFISKYNKEN
                     KNNEDE"
     gene            13729..14715
                     /locus_tag="L3M60_000014"
     CDS             13729..14715
                     /locus_tag="L3M60_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HBN6224725.1"
                     /translation="MNTKAGILGVGSYLPEQSYDNFHFEKIMDTSDEWISTRTGIKER
                     RFAKESEATSDLASKAALKAIECAKLNVEDIELIILATITPDMSLPSTACIVQDAIGA
                     VNATAFDISAACSGFVYGVTIAKQFVETGCYKNVLVIGAETCSKFLNYDDRTTAVLFG
                     DGAGAAVIGPVSEGGILSTHMGSDGKGKDCLKVPAGGSRLKASKETVEANLHTIEMAG
                     SDVFKFAVRKMAETSLRALEKANLNTTDIDYLVPHQANIRIIQASSKRLELDMKKVYV
                     NIDKYGNMSAASIPVALDEAYREGKIKKGDNVVLVGFGGGLTWGASVVKWTL"
     gene            14824..15753
                     /gene="fabK"
                     /locus_tag="L3M60_000015"
     CDS             14824..15753
                     /gene="fabK"
                     /locus_tag="L3M60_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HBN6224726.1"
                     /translation="MNKICKILNIKYPVIQGGMAWVATASLASAVSNAGGLGIIAAGN
                     APKEAIKKEIVECKKLTDKPFGVNVMLMSPFVDDIIDLIIEEKVQVITTGAGNPAKYM
                     DRLKEAGTKVIPVVPTIALAQRMEKLGATAVIAEGTEGGGHIGELTTMVLVPQVADAV
                     NIPVIAAGGIVDGRGIAASFALGASAVQVGTRFICSEECSVHSNYKNLVLKAKDRDAI
                     VTGRSTGHPVRTLKNKLSKEFLKMEQNGATPEELDKKGTGALRFATVDGDIEKGSFMA
                     GQSAAMVKEITPCKEIIEAMVNQAREIMPAIEL"
     gene            15769..16719
                     /gene="fabD"
                     /locus_tag="L3M60_000016"
     CDS             15769..16719
                     /gene="fabD"
                     /locus_tag="L3M60_000016"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBN6224727.1"
                     /translation="MGKVALVFPGQGAQYVGMAKDLYENNTVAKSVIDEASDALNMDL
                     KNLMFNGNEEELSKTENTQPAMVTHSVAVLKAVQAEIDLKYNACLGLSLGEYSALVAA
                     DAIDFKDAVCLVKKRGKFMQETVPTGVGAMAAILGLDRNALESVVKDIQGGIVEVANY
                     NSPGQIVISGENEKIEEAIVKCKEAGAKRAVKLNVSGPFHSSMLKEAGVKLASELEKV
                     NMTKPKVDVVANVNADYYKNEGKELLIKQVSSSVLWEDSIERLLNDGYDTFIEMGPGK
                     TLKAFIKKIASNKKANVNIYNIDGIDSLNEFVQNYRNGEI"
     gene            16719..17468
                     /gene="fabG"
                     /locus_tag="L3M60_000017"
     CDS             16719..17468
                     /gene="fabG"
                     /locus_tag="L3M60_000017"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HBN6224728.1"
                     /translation="MINLTGQVAVVTGGSRGIGKEIAKKLASFGADVVINYTSKEDEA
                     LKTKNEIENMGVKCTSIKCDVSKFDEVNQMIDSVVSEFGKIDILVNNAGITKDGLLMR
                     MKEEDFDRVIDINLKGVFNCTKAVTKPMMKKKYGRIINMTSVVGIMGNAGQTNYCASK
                     AGVIGFTKASARELASRNININAVAPGFIETDMTKVLSDDVKESTLANIPKKSYGKPE
                     DVANAVAFLVSDMASYITGQVINVDGGMVMQ"
     gene            17531..17755
                     /gene="acpP"
                     /locus_tag="L3M60_000018"
     CDS             17531..17755
                     /gene="acpP"
                     /locus_tag="L3M60_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBN6224729.1"
                     /translation="MFNKVVEIIIEQLGVEDKEITMETSLMKDLEADSLDAVEIIMAL
                     EDEFGIEIPDTEAENFKSIGDIVKYIEANK"
     gene            17784..19022
                     /gene="fabF"
                     /locus_tag="L3M60_000019"
     CDS             17784..19022
                     /gene="fabF"
                     /locus_tag="L3M60_000019"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBN6224730.1"
                     /translation="MNKRVVITGLGCVTPLGTGREEFWSNIKAGVSGIDKITNFDAST
                     YQTQIAGEVKNFHPEEYISKKELKRLDKFAQFAVVSAKLAVEDANLDLDKVDRERFGV
                     IIGSGIGGVEAIETQHKILLEKGNKRVSSLFVPMMIGNMAAGQVSIFLGAKGPNTNVC
                     TACASGTHSIGDAFKVIQRGDADIMVAGGSEAAVTGLAFAGFCNMKAMSTRNDDPKTA
                     SRPFDKDRDGFVMGEGAGIVILEDLEHALARGAKIYAEVVGYGLTADAYHMTTPAENG
                     EGAARSMNMALKDGNVPLEEVDYINAHGTSTYYNDLYETMAIKTVFGEKAYDLCVSST
                     KSMTGHLLGASGAIEAVVCAMSIEDSFVPPTINIQEVGEDLDLDYVPNQGKEKNIRYA
                     LSNSLGFGGHNATIVLKKYV"
     gene            19241..20959
                     /locus_tag="L3M60_000020"
     CDS             19241..20959
                     /locus_tag="L3M60_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6224731.1"
                     /translation="MNFRLPKKRLPKKIIFICIILISIVLFIKYDQNGLKEREIISKG
                     KYMSESLVADKSYDEAKKVVKTSFESIPAKKYNKVYNEVWDMLKTISYVPDGTKIVID
                     SLEKMRESDAKLSDECRFNIEKRLASVYIMDNNYSKAVDLTVKSIKLAEKLGNNYEKA
                     RLDVDLASILLKVGSYKTGEKLIKDSFKIDIDEGEKNGMVRLYALINLAEIYVEVGEY
                     DKAIEISSRVKDYKKFVNTDNYNDFEIMASIMQANAYIEKNNIQEAKNLLEKADYIIK
                     SDRTVHILDKDMYYYMAMGNLEYKNENYDSAIDNYKKSLDISTKRKLTQEKIKNLNKI
                     LNIYEKQGNVESLNEYQKMLINEYKENKSIRDSETSFYIIDKVSNESELFEKTKKEIK
                     YYKILFTVILIAVLGSTFLYNRLKYFKAQNLHDGLTNVYNRKSFDIMYEKYRAKDDNF
                     ALVMIDIDNFKLINDNYGHKFGDVVIKGITNTICVMLEQDDKVFRYGGEEFSVLIRNK
                     SGEEVADIVDNIRVAIANKKWNEDVTVTISAGVAHISDSKDVLEEADKNLYKAKQLGR
                     NKVVYK"
     gene            20990..22990
                     /locus_tag="L3M60_000021"
     CDS             20990..22990
                     /locus_tag="L3M60_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6224732.1"
                     /translation="MKQSIAIVTDKLTKLSSFLEENIRLVLGNYIDINHYYLEVLKED
                     DKIQGDFVLVMNDDRLNSMRKHLVQDSKIIVIRRTLKENEIYGLFSIPNGTEVLVVND
                     TKETTLETISLFYKIGVKNLKFTPYIEGKIYKNIDIAITPGVKEKVPSYINKVIDLGN
                     RYIDISTFIEIINGLRIDVKEIRKNLMAYSESLISLDNGVKDNYRQLFLKIEEQDVIL
                     NLSKDGIIFTSADGVINTFNKKALKILGINENPQGKKIEDIISKDLNILLGDKEIIDE
                     VVFTNKKYINVNKKSIFSMGNKAGIYYNLQEITYIKKLEQNLTNKLREQGQIARYTFK
                     DIKTKNKNMIKCIELAKKISKSDLSVLIIGESGTGKELLSQSIHNASNRSNQPFIAVN
                     CAAVPDSLLESQLFGYEKGSFTGALREGKKGLFELANNGTIFLDEIGDMPPLLQTKLL
                     RVLQEKQVMPIGSHNVINIDVRVIAATNKNLPKMIREGKFREDLYYRLNVLPIDVPSL
                     RNRKEDIITLMNFFLKNNIRLSPEVITILEKYNWPGNIRELQNVASYVSLMCDSMVFR
                     DDLPPYISVEFANNYEDGFGYTKKEFKEILRILYERRESSSGVGRGFIIEKLIQEGIE
                     ISESKVRKVLSYLSECGYISSKSGRGGSQITSEGIKLYNNIN"
     gene            23339..23584
                     /locus_tag="L3M60_000022"
     CDS             23339..23584
                     /locus_tag="L3M60_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBN6224733.1"
                     /translation="MKYKTTGTCATEIEFEVKENKVTNVNFIGGCDGNLKGIKVLVEG
                     MNIEDVIQKLKGIECKTKPTSCPDQLSLALENYMNIK"
     gene            23619..24344
                     /locus_tag="L3M60_000023"
     CDS             23619..24344
                     /locus_tag="L3M60_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBN6224734.1"
                     /translation="MKPIIGILGNLIIMENGMFPGLERSYVNNDYINAVLKGGGSPVI
                     IPVNTDKEVIKKQIEMVDGVLISGGWDVNPQLYGEETREETTFIYPEVDEFDLIAINI
                     ALGLQKPILGICRGLQILNVSLGGTLYQDNNLKEGSYIKHTQSSKRHVATHKVDVKEG
                     SILEGILGKQLLTNSYHHQSVNQLGKGLKAIAYSKDGIIEAIEKEDEKFVVGVQWHPE
                     MMVDYCNKMEKLFKYFINICSNL"
     gene            24367..25680
                     /locus_tag="L3M60_000024"
     CDS             24367..25680
                     /locus_tag="L3M60_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN6224735.1"
                     /translation="MNEKNKMGLISIILLGINAVVGAGVFLLPGDAMKAFGVASIFVY
                     MFDMLLVLSMAFCFAEVAGKFNKNGAAYVYTKEAFGDFCGFEVGLMKWVIGCISWGAL
                     IVGFPTSLSAVWAPAGEPHIQKIIIVAMIVGLTIINLLGVSLSKIVQNVITVGKLIPL
                     ILFIGIGIFFIKGVNFTTSTMVPPGAGATEFGAAALLMFYSFTGFESIAVAAEDMENP
                     QKNIPIAIISVIVIASIIYILNQVVCVGILGDSLSSTSTPVADAARVCFGNMGAGLVT
                     FGTLVSVGGICMCGAFVNPRSCVALADDKMLPRIFARKDKKGTPYVAIIATMLITIPI
                     ALSGSFAELAAISAVARFIQYIPTSLSVLVFRKKRPELVGTFKTPFGAVIPLIAVCVG
                     TWLLFQASMHQLIMGLGALAIGVPLYFIMKSYNRKVYGENLKKIV"
     gene            25886..26425
                     /locus_tag="L3M60_000025"
     CDS             25886..26425
                     /locus_tag="L3M60_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6224736.1"
                     /translation="MPTITLKNGVDVLIREGVREDAQNIIDFYNEVGGETHFLSFGKD
                     EYKISLKEQENAIESAKASDNSVKLIAFIDGEIVGIATIDSNQKAKGKHVGVLGIVVK
                     EKYWGIGLGKRLMLDLIEWCKSNGITKKITLVTNEENYNAIGLYKKVGFEVESILKKE
                     CYYNGVYTDLIGMSLLLGI"
     gene            26514..28499
                     /locus_tag="L3M60_000026"
     CDS             26514..28499
                     /locus_tag="L3M60_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HBN6224737.1"
                     /translation="MKNLCKISDDYLNSKLKYLKLLSKQYPSISKASTEIINLEAILN
                     LPKGTEHFITDVHGEYEPFVHVLKNGSGVIKRKIEELFSNTIRDSEKKMLATLVYYPE
                     QKLDLIIKQEENIDDFYRINIYRLIELCKYASSKYTRSKVRKLLPENFKYIIEELLHE
                     HVKSEHKEEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYDRGPRPDI
                     IVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVEDIYGINLL
                     PLATFAIEMYKDDPCKEFIPKINDQSVTTTEKSLMAKMHKAISIIQFKLEGEVIRRRP
                     EFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTIEEKNVIDKLVS
                     SFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGKEYKGKAL
                     MDRMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERYFIAEKET
                     HKENKNPYFKLRENEMACKKLFEEFDLELDESHIINGHVPVESKNGESPIKANGKILV
                     IDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILSTTVVVEHK
                     AKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
     gene            complement(28813..30030)
                     /locus_tag="L3M60_000027"
     CDS             complement(28813..30030)
                     /locus_tag="L3M60_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6224738.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(30112..30315)
                     /locus_tag="L3M60_000028"
     CDS             complement(30112..30315)
                     /locus_tag="L3M60_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBN6224739.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            complement(30776..31006)
                     /locus_tag="L3M60_000029"
     CDS             complement(30776..31006)
                     /locus_tag="L3M60_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6224740.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            complement(31003..31425)
                     /locus_tag="L3M60_000030"
     CDS             complement(31003..31425)
                     /locus_tag="L3M60_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6224741.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            31930..32283
                     /locus_tag="L3M60_000031"
     CDS             31930..32283
                     /locus_tag="L3M60_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6224742.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            complement(32343..32510)
                     /locus_tag="L3M60_000032"
     CDS             complement(32343..32510)
                     /locus_tag="L3M60_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBN6224743.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(32629..34548)
                     /gene="tet(M)"
                     /locus_tag="L3M60_000033"
     CDS             complement(32629..34548)
                     /gene="tet(M)"
                     /locus_tag="L3M60_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBN6224744.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            complement(34564..34680)
                     /locus_tag="L3M60_000034"
     CDS             complement(34564..34680)
                     /locus_tag="L3M60_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBN6224745.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            complement(34925..35857)
                     /locus_tag="L3M60_000035"
     CDS             complement(34925..35857)
                     /locus_tag="L3M60_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN6224746.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            complement(35854..36855)
                     /locus_tag="L3M60_000036"
     CDS             complement(35854..36855)
                     /locus_tag="L3M60_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBN6224747.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            complement(36852..39029)
                     /locus_tag="L3M60_000037"
     CDS             complement(36852..39029)
                     /locus_tag="L3M60_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBN6224748.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            complement(39032..41479)
                     /locus_tag="L3M60_000038"
     CDS             complement(39032..41479)
                     /locus_tag="L3M60_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6224749.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            complement(41463..41969)
                     /locus_tag="L3M60_000039"
     CDS             complement(41463..41969)
                     /locus_tag="L3M60_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN6224750.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            complement(41944..42441)
                     /locus_tag="L3M60_000040"
     CDS             complement(41944..42441)
                     /locus_tag="L3M60_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBN6224751.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            complement(42558..42779)
                     /locus_tag="L3M60_000041"
     CDS             complement(42558..42779)
                     /locus_tag="L3M60_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224752.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            complement(42822..44027)
                     /locus_tag="L3M60_000042"
     CDS             complement(42822..44027)
                     /locus_tag="L3M60_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBN6224753.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(44050..44202)
                     /locus_tag="L3M60_000043"
     CDS             complement(44050..44202)
                     /locus_tag="L3M60_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN6224754.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            complement(44205..45590)
                     /locus_tag="L3M60_000044"
     CDS             complement(44205..45590)
                     /locus_tag="L3M60_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBN6224755.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(45619..46005)
                     /locus_tag="L3M60_000045"
     CDS             complement(45619..46005)
                     /locus_tag="L3M60_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBN6224756.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(46021..46335)
                     /locus_tag="L3M60_000046"
     CDS             complement(46021..46335)
                     /locus_tag="L3M60_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBN6224757.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            46764..47732
                     /gene="spoIIIAA"
                     /locus_tag="L3M60_000047"
     CDS             46764..47732
                     /gene="spoIIIAA"
                     /locus_tag="L3M60_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBN6224758.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKISNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMALDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKVIVEDGQVKNIKHISSLNIRVSREIIGCSDKILSHIIKGKNQINNTLII
                     SPPQCGKTTLIRDIVRNLSNGNEDYGFKGLKVALVDERNEIAGAYLGVPQMDVGIRTD
                     IIETCPKDLGITMLLRSMSPNVIVTDEIGSEKEIKALYTALNGGIGLITTVHGDSIDD
                     IQNRKELNRLLDKELFKKVIILSAKRGAGTIEKIYDLEEKRWYFAN"
     gene            47722..48243
                     /locus_tag="L3M60_000048"
     CDS             47722..48243
                     /locus_tag="L3M60_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HBN6224759.1"
                     /translation="MQIKIIIIALLIGSSYLIGEQIYKTYTRRHKQLNDLIRVLEILR
                     MDLSFGLYTLEEIFNRIGRNKEFCFWKFFYQISEGLHNEQSKTLEIIISENIDVLSKE
                     TYLGNKEIEELKNLILTLGKSDIESQQRMIDLSIENLKKQTYETKEDINKKGVLYKKL
                     VTFIGIGICIILI"
     gene            48260..48457
                     /gene="spoIIIAC"
                     /locus_tag="L3M60_000049"
     CDS             48260..48457
                     /gene="spoIIIAC"
                     /locus_tag="L3M60_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HBN6224760.1"
                     /translation="MEISLILKVAGVGILISVLNMILEKTDRKDWAGLTTLAGVIIVL
                     GMVITEISDLFNTVRTMFQLY"
     gene            48473..48859
                     /gene="spoIIIAD"
                     /locus_tag="L3M60_000050"
     CDS             48473..48859
                     /gene="spoIIIAD"
                     /locus_tag="L3M60_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HBN6224761.1"
                     /translation="MEIMQLIGIAIISTTLCLVIKKDRPEIANFIAIITGVIILLSVM
                     FKLNFIVDSVQDLANKANIPTMYISLIIKLIGIAYLMEFAIQLCKDCGEGNIASKLEF
                     GGKIIVMSMSFPILLSIVEMVVNIIP"
     gene            48944..50149
                     /gene="spoIIIAE"
                     /locus_tag="L3M60_000051"
     CDS             48944..50149
                     /gene="spoIIIAE"
                     /locus_tag="L3M60_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HBN6224762.1"
                     /translation="MKKRFLPMIIGFLFTFFFVNIFAIMIFANEVPSSENKEYGETKN
                     SIDKYIDGQLDKLDINEIQDYINKEIVINDVNLKSFVKDLISGEKNILDLFNKDGLKI
                     LMFDEFKASLKVVAVILVLALLSSILKSLENSFSSGAVSQIATYIIFITMVSLTLVGF
                     KDVLQICYDAIDHTVGLMQVIMPILITFLLLIGFPITSTTLNPIFIGGVTFINVFFKN
                     FLFVSITVAFGILIINNLSKNIRLKRFFSFVKQINYVSIGAMFTIYLGLVSIQGLYVT
                     SFDKFSVKTAKFAIGNFIPVVGGFVSDSVDILLSSSQLIKNIFGGIGLILLVGICLLP
                     VIKILSVIVVYKLAAIIVEPVGEDGISNFLNEVANLMIIMLASVIAITVMFFVTVAIL
                     TSISVVSQG"
     gene            50208..50825
                     /locus_tag="L3M60_000052"
     CDS             50208..50825
                     /locus_tag="L3M60_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HBN6224763.1"
                     /translation="MLEGIKAWIVSVLIGAFIVNIVDMILPSSKIKPYVNLVLNFMFV
                     FIVITPVVGFFSKDMSLEDRILKSMGSYNKQYVDSTNALAKETGNNSLSKGYEDGLKE
                     VLKLKLDEYGYDLEDIELNGANINNIKIKEKNNNAKSNSSANEENKNNSTKREEENIN
                     SNDKENSKQVFKKGTEYGLSLNEEKLKNDLIKVLDVSIEDIQIDK"
     gene            50837..51391
                     /locus_tag="L3M60_000053"
     CDS             50837..51391
                     /locus_tag="L3M60_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AG"
                     /protein_id="HBN6224764.1"
                     /translation="MLKNLNDKDKRKVYSLITIAGICVVSLVLLSCFPSSKNDKQVGK
                     TETNKNAEKQVTKEQEKDDLESKLTAILSKIDGAGDVDVMVTFESSEEIQPAFNSNNT
                     TETTEEKDAQGGERTVTTSSENKTMITSNSSDPVVIKTTEPKIKGVIVVASGASDPSV
                     KETLYSAVQTSLQVAGHQVEIYSK"
     gene            51418..52104
                     /locus_tag="L3M60_000054"
     CDS             51418..52104
                     /locus_tag="L3M60_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HBN6224765.1"
                     /translation="MKFNYKGRGFVIITLTAMLVVVGTVNYQLSKKSLLETSKEFKAY
                     EEAQLQKNTDDSDSSKTEDSNNVDKQDGKESADIDIVDSKTSKVKEKATETSKEIKAQ
                     LSSEKNMKKASYILDMKMNREKQRNELVQDLNEMINNPSTTEESRKEASNMKLNIVKI
                     QEKELQIENLLSTKGYEEALVYISDNKVNVVVNEAKLEKKDAAKIFDLVAEQANVKYE
                     NIKLTNNNNK"
     gene            52214..52576
                     /locus_tag="L3M60_000055"
     CDS             52214..52576
                     /locus_tag="L3M60_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBN6224766.1"
                     /translation="MEDNKFGQVKISNDVIATIAGLAALEVEGIETTATLTDKLLKNN
                     GVKIQIEEEDVNLDVMVTIKYGMSIPDTAFKVQENVKNTVETMTGLKVSQVNIHIQGI
                     SFKKDKVDKEEAKATKKN"
     gene            52661..53167
                     /gene="nusB"
                     /locus_tag="L3M60_000056"
     CDS             52661..53167
                     /gene="nusB"
                     /locus_tag="L3M60_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBN6224767.1"
                     /translation="MKKDRAQKSTTREYIMKLIYQININKEDFETLEDRVDNFLKDNS
                     EHIINRYKELALQYSKNTNLKLEDTEIEDVIDKKYINTVCKALKENHDKIDELINKHA
                     KNWTVDRMPKVDVSILRLSVCEILYLDTPNKVSINEAVELAKIYCDDKSPKFINGILG
                     SVVDEIGK"
     gene            53151..54221
                     /locus_tag="L3M60_000057"
     CDS             53151..54221
                     /locus_tag="L3M60_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HBN6224768.1"
                     /translation="MKLGNNIIIGIDTSCYTTSIAAISLDKKVIFNEKIMLEVMDNSK
                     GLRQSEAVFQHVNNLGILSDRIKSFKDRFHVEGVCSSKKPRPVENSYMPVFNVGHNFG
                     RLLSSIYGCRFYETTHQENHIEASLLNSKLKNNNRFISVHMSGGTTEILLTSKQDSHH
                     NVCDIDLSKFAKTSIKKDDKSKLYNNFGYNIDIIGGSKDISFGQLIDRVGVKLGYKFP
                     SGKYLDENALNCNLKIESGLKTSVRDGYMNLSGLENQVNKIIKDNGDNANQKEYISKL
                     VLDSVVRNMFKSLVYLCETYNVSEVVFAGGVSASKYIFRELTMKLGKKHIEAYFTEPQ
                     YSTDNAVGCAIIGLNNFLGERV"
     gene            54218..55423
                     /locus_tag="L3M60_000058"
     CDS             54218..55423
                     /locus_tag="L3M60_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBN6224769.1"
                     /translation="MKLRALDISEANSYIKRILINDPILSNLKVKGEISNFKVHSSGN
                     VYLSLKDETSKLNCVIFKSNFNRNLKLDNGIKVIANGYISVYERDGAYQLYINEIEIE
                     GIGNLHIEFNRLKEKLNKEGLFDPKYKIPIPKMPNSIGVITSPTGAVIRDIINVIRRR
                     YPKVNIKLYPVTVQGDKSAEEICEAIRFFNHMKNVDTLIVGRGGGSIEELWSFNDEMV
                     AREVFNSQIPIISAVGHETDFTICDFVSDMRAPTPSAAAEIATPSLDDINYKLGNIKS
                     RLSKSLTNQIELDQYRLEAVFNKINNYLDSYTIKDKVIQLDKIYDKIIFGIENNLRLE
                     DEKLVKIGALLHNLSPLATMDRGYSIAQKNGKVINSIKGLKIKDSIDIVLKDGNLECM
                     IEKIENKEV"
     gene            55425..55640
                     /gene="xseB"
                     /locus_tag="L3M60_000059"
     CDS             55425..55640
                     /gene="xseB"
                     /locus_tag="L3M60_000059"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBN6224770.1"
                     /translation="MNLTYEEAYKRLESILNELESKNASLDESLSLYEEGISLYKHCN
                     KLLDDAKLKISKFNQLGIEEDFKIEEE"
     gene            55643..56530
                     /locus_tag="L3M60_000060"
     CDS             55643..56530
                     /locus_tag="L3M60_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HBN6224771.1"
                     /translation="MEFKQCLKEKASFVEKVLKEYMPKEEGYQKTVIEAMNYSLSAGG
                     KRLRPILTLEACKIVGGNEDEAIPFAIAIEMIHTYSLIHDDLPALDNDDLRRGRPTNH
                     KVYGEAMGILAGDALLNYAFEVMLAGSINKENPEKYLKAINEIAKGAGIYGMIGGQVV
                     DVESENKQIEKEKLDYIHMNKTAAMMVGCMRAGATIGGANSEQMEEITKYAKNIGLSF
                     QIVDDILDIVGDESKLGKKVGSDIENHKSTYPSLLGLDKSKEIAHNLIDEAKKSIEKL
                     SDDVDFLNGLAEYIIDREY"
     gene            56560..56997
                     /locus_tag="L3M60_000061"
     CDS             56560..56997
                     /locus_tag="L3M60_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HBN6224772.1"
                     /translation="MDFFSEIFNNGALGISLIACFLAQFIKIFTGKEKRIELSRILIS
                     GGMPSSHSSFVTSLATVVGIEKGFNSTDFAIITVLALIIMYDAAGVRRAVGKQATILN
                     QMVADIQHGKHIEQKKLKELIGHTPLEVWFGALLGVVTALILM"
     gene            57031..58896
                     /locus_tag="L3M60_000062"
     CDS             57031..58896
                     /locus_tag="L3M60_000062"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBN6224773.1"
                     /translation="MYKYLDKVNSPKDIKNMSIEEMDLLAKDIRKFLVKSVSKTGGHL
                     ASNLGVVELTLALHKVFDSPKDKIVWDVGHQSYVHKIVTGRKDCFVSLRQFNGLSGFP
                     KESESPHDIFDTGHSSTSISIATGIACARDIKKENYSVISVIGDGSITGGMALEALNQ
                     LGYINTNMIVILNDNEMSIDKNVGGMSKYLSSIIRNSTVEKMTDEVDKILNVTQTGEI
                     LSKTAHRFKDKLMYSFSPQDCSFFDSLGIRYYGPIDGHNTKDLIDILRKAKHKKGPVL
                     LHVITKKGKGYRFAEEQPDKYHGVSKFDIKTGVTSGKVKSMSTSVGEKLVDMANNNEN
                     IVAITAAMPSGTGLNLFESAYPKRYYDVGIAEQHATGFAAGLAKNGMKPYFAVYSSFL
                     QRAYDQVIHDVCITKKPVTFLIDRAGLVGNDGETHHGMFDLSYLNSIPNIVVMAPKDT
                     RELELMMDLSLKLDCPLAIRYPRGSSYYLDKGEYGEIVLGKYEVLDNGQDTVILCIGS
                     MVKHALEAKEILSREGINPTIVNARFLKPIDEDMLKVLLKNHKNVVTIEDNIVTGGFG
                     SRINKFIIDNEYNVNILNIAIPEEFVKHGNADELYDFVGLSPKSIADKIRKLFIK"
     gene            59029..59841
                     /locus_tag="L3M60_000063"
     CDS             59029..59841
                     /locus_tag="L3M60_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HBN6224774.1"
                     /translation="MKKRIDLLLVEQGHFESRERAKKAIMAGLVFVDNQRCDKAGTEV
                     KEDCNIEVKGNPIPYVSRGGLKLEKAMKNFDLTIDGKVCMDIGASTGGFTDCMLKNGA
                     IKVFSIDVGYGQLAWKLRQDDRVVCMERTNIRNVTIEDTKQFADFASIDVSFISLKLV
                     LPKAKELVRHDGEIVALIKPQFEAGREKVGKKGVVREKSTHIEVIKMISDFSVENGFE
                     ILGLDFSPIKGPEGNIEYLIHLRNGNDGYEFDGEIYNNKIVEVVEASHNLEK"
     gene            59861..61540
                     /gene="recN"
                     /locus_tag="L3M60_000064"
     CDS             59861..61540
                     /gene="recN"
                     /locus_tag="L3M60_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBN6224775.1"
                     /translation="MILELYMKNCALVEELRLNIDKNLNILTGETGSGKSIIIDALGL
                     CLGDKYDRSFLRKGTDKGLVEAVFFSDNTYLKKILEENDISIEDDNLLVITRLIYSDG
                     KSTARVNGRTVKVSLLKEIASTLIDIHGQHQNQALFNKDTHLKFLDLFGENELEEFKI
                     AYKNVYHKYSEVKKALNCLTENKDEMQIQREIDLLRFQINEIEAANLNKNEYEDLLKQ
                     REVYRNSEKIYNNLNSSYSKLHNGEYNVIDLIGLASKELNDISKYDSVLSEYSDTVER
                     IMYELQDISGEIRNYKDNIDFEPYELEQIELRIDEINNLRRKYGDSIDDIFEYYRKIK
                     DRLDEILNRDERVEQLKSKLMKIEEDLKIKASKLTKARIEVATTLEKILLDELKSLNM
                     KNVMFKVNFEKGLFTLDGVDDIEFMISFNLGEDINPIYKVASGGEMSRFMLAFKTILA
                     DIDDIDTLVFDEIDTGISGIAAQIVGEKLSDIAKKKQIICITHLPQIAANADTHYCIE
                     KDTSNNRTFTNVSKLNESQRKNEIARLIAGNNITEKTIEHASEIIELAKKC"
     gene            61755..62621
                     /locus_tag="L3M60_000065"
     CDS             61755..62621
                     /locus_tag="L3M60_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224776.1"
                     /translation="MNKKDTLHLIFIIVIFILLWYVVVTVSEINKNIESCSNKLDYLS
                     QEIMSTKSDISNTINEEMSKSYITKSIDLKVKKIEKKECIIDVDVQLSKLGENGKVFF
                     MYKEESNKWNETEMTKHGELSYVCEIKINTGSEYDYKVVTSGAISESSDVQKLTKSDY
                     MPEQPIFSSGIRDNREYFIEMVENSNLFNHESEKSKNKAYNNIRLEKVDIIVNEGKKD
                     TVYKAKLVESLDDGKTNNSNRNQVNYEASFPKRDIDDIIYIKAKLTYNNGVEYTKDIT
                     EDITMGLQDLEN"
     gene            complement(62714..63205)
                     /locus_tag="L3M60_000066"
     CDS             complement(62714..63205)
                     /locus_tag="L3M60_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6224777.1"
                     /translation="MADKMAQNITLQFVEEKDLENLENVKVLFTEYSNSLNIDLCFQD
                     FNNELKTLPGKYKKPSGSLILAFVDENLAGCVALKKLEGKICELKRLYVRNQFRGLKI
                     GKILLEEIIEEAKKIGYTHMRLDTLPSMKSAQGLYKKFGFYDIEPYTYNPIEGARYME
                     LKL"
     gene            complement(63266..63706)
                     /locus_tag="L3M60_000067"
     CDS             complement(63266..63706)
                     /locus_tag="L3M60_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6224778.1"
                     /translation="MKLNVRIADINNWRDLVNLSVDKSQLDYIESNALSIAESKFITA
                     WIPVGIYDENSLIGFAMYGRLEDDRVWLDRFMIDSKYQGRGYGKASLDFLVNHLKKEY
                     NCDELYLSIFEDNKMAIKLYKDFGFEFNGELDYGGEKVMVLKSN"
     gene            63867..64925
                     /locus_tag="L3M60_000068"
     CDS             63867..64925
                     /locus_tag="L3M60_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBN6224779.1"
                     /translation="MNKINKNFKKIWIILGAVSIFTMVIIFNKNCNVNKETQIEAFTI
                     KNQDKKFVYPMGNIIGVKANTDGVLVLGYEEEDVDYIGGIQIGDNIVKIDNKRIKNSQ
                     DVSEILNKIKKSKVEVTFERKNRYKTEVIETKKENGKYKLGLWVRDKISGIGTMTFYD
                     PSMEKFKAIGHAIKDSDTNELLKIKQGYIYKPEQLKIVKASNEKVGKIKGDFNDSNLM
                     GNFSNNSELGISGNITENHNKEFNVANKEKQLIEVGRPQDVKIGDAVILFEDKNKNIT
                     SYDIKIESIVYDKGNYRDMVIKVVDDKLLEYTGGIVQGMSGAPIIQNNKIIGAITHVF
                     RDNPKKGYGIFIDEMIKL"
     gene            65067..65891
                     /gene="spo0A"
                     /locus_tag="L3M60_000069"
     CDS             65067..65891
                     /gene="spo0A"
                     /locus_tag="L3M60_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HBN6224780.1"
                     /translation="MGGFLVEKIKIVLADDNKDFCQVLKEYLSNEDDIDILGIAKDGI
                     EALDLVKKTQPDLLILDVIMPHLDGLGVIEKLNTMDIPKMPKIIVLSAVGQDKITQSA
                     INLGADYYIVKPFDFVVFINRIRELVSNRVTQVEPKPRPVQETQMTRSDFVKNVGNIE
                     TEITNIIHEIGVPAHIKGYLYLREAIKMVIDNVELLGAVTKELYPSIAKKFNTTPSRV
                     ERAIRHAIEVAWSRGKVDTINQLFGYTVHNTKGKPTNSEFIAMIADKLRLEHSMVK"
     gene            66303..67424
                     /locus_tag="L3M60_000070"
     CDS             66303..67424
                     /locus_tag="L3M60_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224781.1"
                     /translation="MRVEAPIKVDRKTKKLAKRVESGEIAVINHIDIDEVAANSLVEA
                     KIKLVINAAPSISGRYPNKGPGILTENNILIIDNVGEELFEELKEGETIEVVDGKIYR
                     KGKFLGAGEVLDKYEVSYQIKAAYENLAVELDRFIDNTIDYAKKEKGFILGEVEIPKV
                     KTNYANKHVLIVVRGQDYKEDLSTMLSYIEEVKPVLVGVDGGADALIEFGYTPDVIVG
                     DMDSVSDEALKKASEIVVHAYTDGRAPGLKRVEELGLDAVVFPAPGTSEDIAMLIAYE
                     YKAELIVAVGTHSNMIDFLEKGRKGMASTFLVRLKIGSKLIDAKGVNLLYRSKLKIKY
                     IWALIATALFPVLVVASLSPGVQQFIQLMQLKFRVLLQM"
     gene            67442..68317
                     /locus_tag="L3M60_000071"
     CDS             67442..68317
                     /locus_tag="L3M60_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HBN6224782.1"
                     /translation="MHINMKYYIVTIGAIFIALGIGMLVGFNLNNNQQLSEQQANIIN
                     DLDDKFNILKEKNDKLDSDLANVNRDYEEAVNFINKNVDKILAGSLNGKSIGIISANE
                     NDDYTKNIEDIINKSNGSIAFNIVLKENITNPEKLKEMSTKLGTDVKNANDAVNYIID
                     TLKKEDASDILTYLQELDVIKFNSLGNTYLKYDSVVIAGGNDAKDSTKQFEKIEKFVV
                     SKLKSENKYLVEVQNTGVKTSYVELYSKNKVATIDNIDEGIGSISLAILLQQGNIVGN
                     FGRLDTATSLLPSIK"
     gene            68341..69033
                     /locus_tag="L3M60_000072"
     CDS             68341..69033
                     /locus_tag="L3M60_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6224783.1"
                     /translation="MDSYISIIIPAYNEESRIKYTLESILDINQINEIIVVDDGSIDN
                     TAKAVSELENEKIKFFKLDKNRGKGYALNYGIKLAMKNADVIGFLDGDLGSSAKEVEK
                     LIVPILNGEADVTIAKFPPAKRKGGLGFVKGLAKNSVFEMTGVELDATLSGQRIFKRE
                     VLEKFDEIPFGYGVEVGMTIDILKYGFKIKEVLVNMTHNETGRNLKGFIHRGKQYYHI
                     KKVLGQKKKEWR"
     gene            69041..69865
                     /locus_tag="L3M60_000073"
     CDS             69041..69865
                     /locus_tag="L3M60_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBN6224784.1"
                     /translation="MKDYILYTILLLTGLFGTYAVIPLFKNLLINGNVLRPNYKKDMI
                     PVSMGIVFLPMIVINGIILAFFTTEFKDLAYIFMFMFGMISMFFAGILDDVIGNRDVS
                     GLKGHFKSLFKGSLTTGGFKALFGGFVGLIVSVAISKDIIDIIVNTLIIALSTNLMNL
                     LDLRPGRAIKAYLFIMVIIFLTLTGFVQVLPLLIVPNVLAYFNYDLKARAMMGDTGSN
                     VLGISIGMLISFGYPFNIRLGWLIFVVIMHIFTEKYSLTKIIEKNKLLNFIDKLGR"
     gene            69870..70937
                     /locus_tag="L3M60_000074"
     CDS             69870..70937
                     /locus_tag="L3M60_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HBN6224785.1"
                     /translation="MISKRVGIVESIVSQTETLDDIRVNINGEIQRAYNYPNISGTIN
                     IGDEVVLNTTAVELSLGTGGYHFVITNLNNIESTLTEGGHIMKLRYTPLQIKVDSVEE
                     QESIYHDKFANFKGLEGLSVVVGTLHSMLTPFVASFKRNNPNKKLVYIMTDGASLPIY
                     LSKNVDTLKEKKLIDSTITIGNAFGGDYECINIYTALITAKEILKADVVFVSMGPGIA
                     GTGTKYGFTGIEQGSILDAVKKLEGRAIAIPRISFADKRERHQGISHHSMTVFKEIVN
                     VNVDIPITIYDDEKLNFIKEQINLNGLNKKHNIIYIDNNKTKEDLDYFNLKVKSMGRN
                     YEQDEAFFKAASTAAYYLMEV"
     gene            70939..71475
                     /locus_tag="L3M60_000075"
     CDS             70939..71475
                     /locus_tag="L3M60_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBN6224786.1"
                     /translation="MVLEEKTISSDRVYTGKVITLKVDTVEIPGQGYQKRELVEVGGA
                     VGIVAITDDNKVVLVKQFRKPIEKPIFEIPAGKLEKNESPKECAERELKEETGYSAKN
                     VKLIHKFFTSAGFSNEIMFVYLATGLTPGENNLDADEFLDVHEIELEEAYNMVLKNDV
                     EDAKTSIGLLLVKDMFKN"
     gene            71543..72148
                     /locus_tag="L3M60_000076"
     CDS             71543..72148
                     /locus_tag="L3M60_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HBN6224787.1"
                     /translation="MRRTYRKNDFRELNRQIIIIGLLFMMSIVVGSYINKILPSSSNN
                     ILNNINPAVEYYNLNISIKDAVIQNLKSDAIFMGSIALLSLFVVTIPAVLVAFVLKGM
                     SIGYTINSCILALKFKSIKMILIILFKNLIIIPCAIILTLISLSYFKEMVYEFKKKNR
                     KNMQFLIKRYILNIIIIIALSLGLQLILNTVSIGIIKFLAK"
     gene            72160..73073
                     /locus_tag="L3M60_000077"
                     /pseudo
     CDS             72160..73073
                     /locus_tag="L3M60_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            73115..74287
                     /locus_tag="L3M60_000078"
     CDS             73115..74287
                     /locus_tag="L3M60_000078"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBN6224788.1"
                     /translation="MSRVIWIVIDSVGIGALPDAENFGDSKDVSTLGNIFREYPDINI
                     PNMRKLGIGNIDGVDFFESIKTPIGCFGKCKEMSQGKDTTTGHWEMTGIIVDKPFKTF
                     EHGFSKEIIDEFEKKTGRKVVGNKPASGTVIIDEYGEHQIKTGDVIVYTSADSVFQIA
                     ANEEVIPLEELYNMCKIAREIMMGDNAVARVIARPFIGKKKGEFVRTSNRRDYSLDPF
                     EPTVLDNIKESGLDVLAVGKIEDIFNGKGITDAIHTKSNMDGVDETLNYMKQDNKGLI
                     YSNLVDFDSKYGHRRDPEGYKKALEEFDSRLPEIMANMREDDILIINADHGNDPTYKG
                     TDHTREYIPVMIYGNKIKKGFNLGVKDTFADIGATVADILNVKLPKHGSSFKKDLF"
     gene            74334..75146
                     /locus_tag="L3M60_000079"
     CDS             74334..75146
                     /locus_tag="L3M60_000079"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBN6224789.1"
                     /translation="MFEKIAQSSKFINAKINIKPKIGLILGSGLGDLANDIEDSVTIK
                     YSEIPNFPISTVAGHAGQLVIGKLEGKEVIAMQGRFHYYEGYSQKEATFPVRVMKELG
                     VEVLIVTNAAGGVNKEFKAGDLMLIRDHINFSGSNPLVGKNDDRLGDRFPDMSDAYSS
                     RYVDVVKNCAKECNIDVKEGVYMFFSGPNYETPAEVRMAQILGADAVGMSTVPEVIVA
                     SHSNIGVIGISCITNMAAGILNQSLSHEEVIETTQKVKSEFMNLVKTTVKNL"
     gene            75349..76674
                     /locus_tag="L3M60_000080"
     CDS             75349..76674
                     /locus_tag="L3M60_000080"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HBN6224790.1"
                     /translation="MRMYDIIRKKRDNLELSKKEINYFIENYSKGDIPDYQASALLMS
                     IYLNKMNKQETVHLTEAMMNSGDMINLSEIDGIKVDKHSTGGVGDKTTIALIPLVASC
                     GAPVAKMSGRGLGHTGGTIDKLESIPGFSTEMEISKFINSVNKSKIAVCGQSAKVAIA
                     DKKIYALRDVTATVDNISLIASSVMSKKLASGADAIVLDVKTGDGAFMKTLEESFDLA
                     KSMVDIGTGMNRDTIGIVTDMDEPLGFAVGNSLEVIEAIETLKGNGPKDLVELCEVLG
                     AYMLVLSKVAYDFDDGVEKIRESIKSGDAIHKLKEFIENQGGNKDVVDDYSLFKKSKY
                     KYPVKSTKSGYINKIKAEDVGVSAMILGAGRETKDDVLDLSVGIILEKKVGDYINEGE
                     VLAYMYYNDEQKLSLAKDRFIDSYSIVDSKVEKNKLIYGIVTKEGIKKF"
     gene            76887..77966
                     /gene="mltG"
                     /locus_tag="L3M60_000081"
     CDS             76887..77966
                     /gene="mltG"
                     /locus_tag="L3M60_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HBN6224791.1"
                     /translation="MNFKENRLKIAALIIVILIILAGIFVSMQIGPYNKNNKKDVIID
                     IPNGASIGKISDILYENKLIKNELLFKLLVKVSNKAPSIKSGTYLLNQSYSNNDIISL
                     LVSGKIYQDGIKVTIPEGATSKEIIAMLVSKNLGDKATFENLIKKPQEFYDKFPYLKE
                     DGITSLEGFLYPETYYFNSKKQSEEDILSEMLKVFDSKYTDKFKKKQKKLNMTLQEVM
                     EMASIIEKEAVLDKDRPIIASVFYNRLKVGMPLQSDATIQYIFEERKKIVTYDDLKID
                     SPYNSYKNKGLPPTPISNPGIKSIEAALYPDKTDYLYFVAKIDGGNNYSTNYQDHLKY
                     VKEYKEARDKQSKDTKATNKENTKK"
     gene            78138..78812
                     /locus_tag="L3M60_000082"
     CDS             78138..78812
                     /locus_tag="L3M60_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HBN6224792.1"
                     /translation="MSNIVNDLVEDYIRVTLKEKEGFLKDLEIYAEENSVPIIHKEVS
                     DLLKVLLKIQKPKRVLEVGCAIGYSSIFFATIIGKDADIITVERNEKMIEKAKENIKL
                     AGFENNITILEGDAEEKLSQVQGEFDLIFIDAAKGQYKLFFDLVIDKLKDGGLLVSDN
                     ILYKGMVAHDDFVVRRKKTIVKRMRNYLDYICNCDYLSTSLIPIGDGVALSYKESKRG
                     DVDGIK"
     gene            78799..80046
                     /locus_tag="L3M60_000083"
     CDS             78799..80046
                     /locus_tag="L3M60_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBN6224793.1"
                     /translation="MELNKVELLAPAGDLERLKIAITYGADAVYIGGEIFGMRSAAKN
                     FSKEDMAEGVAFAHERGKKVFVTVNIIPHNEDFLQLEDYLLELEEIGIDAVIIADPGV
                     LSVIKKVIPNMEIHLSTQANTTNYLSANFWYEHGIKRVVVARELSFDEISEIRAKTPL
                     DMDIEAFMHGAMCISYSGRCLISNYMTGRDANKGSCAQSCRWQYHLVEEKRPGEYFPI
                     YEDERGTFFFNSKDLCMIEYIPELIKSGITSLKIEGRMKTAYYVATVVRAYRMAIDEF
                     YKDPENWKFNPMWMEELKKGSHRHFTSGFYLNKPTTEDQNYQSASYVRNYDFIGIVRE
                     TEDEDGLIVVEQRNKMCVGDEIEVMGPYKETMFTKIEAMYNEEGEAIESAPHPRQIVK
                     LKLSVKVGKDYMLRKVIEEKVEE"
     gene            80118..81782
                     /locus_tag="L3M60_000084"
     CDS             80118..81782
                     /locus_tag="L3M60_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HBN6224794.1"
                     /translation="MSKKKTPFLKKVGKRSWCVFTIILIIYSALIYRLVDIQVLKGDK
                     YKQSVESQSVEKVELNSGRGIIYDRNNKKLTDTSKYQVLVVEKEKLNNNYKILELIKK
                     ATKMNDLDIYKAVQEQLTRPIIQIQAKNIDKNMKKELEKNGIMIEEKTMRYSKDGLLS
                     HTIGYIKEDDKSGQSGIEKSMDSVLRNSNEKYISAFKAGDAGNEKSLNILKGSVKTID
                     NKDKDRHLKTTIDYNIQRKLEQILNKEENPTAAIISEVSTGEILAMCSRPNFDQNDIS
                     KSLKGKNGEFENRVIKATYPPGSVFKMVVLFSALENGVIDENYTYNCTGKTKVGNTNE
                     ILRCNKRDGHGFQNLRQAFSNSCNPAFLDIAMKLGKEKILKSAEKLHLFEKVDIGLDE
                     EKIREAPKNISIRNLAIGQENIEFTPLQINQMTQIMANNGTFKPLYLYKSLVDNDMNT
                     IKTYKSSKKEELISPYVCTQVKEYMKSVSRTGTAKDLKDIEGGCGVKTGTAQSSLNKK
                     AIDHGWITGFYPEERPKYVITVLVEGTQKGNKSATPIFKEICESIQ"
     gene            complement(81880..>82139)
                     /locus_tag="L3M60_000085"
     CDS             complement(81880..>82139)
                     /locus_tag="L3M60_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6224795.1"
                     /translation="ILGTEANYVLDEVELKVQVGKLYEQLNKILTPREREIVQLRYGL
                     TPYGYKTQREIAQKLDISRSYVSRIEKKALKKLEKELVQES"
BASE COUNT        30959 a        10384 c        14990 g        25806 t
ORIGIN      
        1 agtgttgaaa taatgaaaat atatttgact ttataaaaaa agagtgtctt atgaactttt
       61 tagttcataa gacactcttt ttttatatct ggtgaattaa taagataata gaaaaatgta
      121 tacgttataa tttaaccaat aaagaaattt tcactagcct cattaatagc ttgctgagtt
      181 acccttatat atcgctgtgt agtagccaca gagctgtgat ttaaaagctc cttaaccaac
      241 tctaaattat tgttactctt ttcaaattga gtagtagcat agagctttct aaagctatgt
      301 gtacttatat tatacagatt taagtgttta caaattatcc tcaactgttt ctgaatagct
      361 tttgtcttta tatttactaa gtagtcattt gatttaagat tatactttct tgcatgttca
      421 tttatggact caattatatt tttattaact ggtctatact gtaatttgcc tgtctttttt
      481 tcatttattt ctaacatatt acctttaaaa ttacctatct ttaatcttaa aatatctgat
      541 attcttagac ctaaattagc ttctaacatt agtgccaatg caactttagg attttttcta
      601 aatctttttt caacgccatt ttcactataa gtaaagccag tatgtaatag ttccattatc
      661 tttttatatt cgtctatttc tataggtctt gttgccattt tcaaccactc ctcttagttt
      721 gatttggtaa gtttaggtcg gtatttatta tataatataa atgaaatatt atataatgga
      781 aaaatttgcc agtaaactta tacaagtgtg atatatgtta ttaaggagaa tttttgaaaa
      841 atatttctaa taaaattatt aaattgtgat gaaataacta tgttgttaaa ctaattaaaa
      901 aggttattaa aattatgaca aatataataa aaaaatggaa gtatttagta aagttttatt
      961 atttttattt ttttcgtgaa aatttcttga tgaaaaaagt atttttatat ctcattagtt
     1021 tcccatttgt agataaagct ttcatatgtt tttttgcaat aaatacggct gagattagat
     1081 aaattattat tacaatttga agaattgtat cgtaatagct aagataataa aaccagatta
     1141 aattattgta tataacgttg tatgtatcag caaagctaga aactaataca gcagagtttg
     1201 caattggata aagtataaat ataaagatag cagctaaaat tccatgtgca aacttactta
     1261 gtatgtataa ttttaaatct atgtctgttt ttgatagaaa tgtacaacac tgagctagaa
     1321 tagataatcc actaaatcct atcaaaaagc tagctaaaga tgctcttaga atttctggag
     1381 cacttgttat actagatact ttattacatc ctatagtaat ttcaaataga ccacttacaa
     1441 atgcttgaca gagttcttta ctaacaccta aaaatgaaag cggtaaatat ataactgatg
     1501 ctattaatga gattacatta aaaagagaga gtattttaaa taccactgaa aaaacaatta
     1561 caaatccacc aactgtaagt aatgtattta ctccgttaaa tacagcattt ccaaataaaa
     1621 caaaaaaacc attcccactt gaatttctaa tgttgattac attttttatg tttgttttta
     1681 aatctaattt cgtctttgga agactttcat taccataatt tctaaagaaa agaccaacca
     1741 atatagtccc tagataatga caaagaatca ttaaataacc taagtttgaa ttttggaaca
     1801 tacctgtacc aaccgagcct attatgaata aaggtcctga tgtagaacaa aaagacacta
     1861 gtctttgtgc ttcatgctta gaaatttgcc tattactcct aaattcagaa gctaaactta
     1921 ctcctactgg ataaccagat actgtagata tagcaaaagc taaagcgctc tttcctgaaa
     1981 cattaaatac aaatctagtt atgggattga taataaaccc tataatatcc actattttta
     2041 aatcaacaat taagtttgct ccaattataa atggtagtag tgatggaact aaaacattag
     2101 accatataga gaagccttct cttgcagatt ttatagcttc atttgggtat aggataatcc
     2161 ctaaaactag aactccaact ataatagctg gagcaaaaaa agcaataatt ttattttttt
     2221 tcataatatc acctgtaaat ttgagttata agatattata ttaaaagtaa taaaaatata
     2281 tgaatgtaat ggcttaagat atataataga tatatgttac aacaacgatt atcatactgg
     2341 agggaaaaat gtttaatagt atagctagta aaaaagttta tgaacaagta attgagcaaa
     2401 tacaatataa aatactaaat ggggagttaa aaaaagggga taaattacta tcagaaagag
     2461 aactatctga acaaatgaat gtgagtagaa cttccatcag agaagctatt agagtattag
     2521 agactatggg agtgatagaa agcagacaag gggaaggaaa ttttatatgt acaaatatag
     2581 aaaaaacttt aatagaacct ttatctatga tttttaaact taataatggt acacttggag
     2641 atatattaga gcttagaata atcctagaaa ttgagattgc aaaacttgct tcaaagagaa
     2701 taacttcttc agaagctata gaattgaaaa atataataga tgaaatgagg gtagaaacta
     2761 ataaaaaaga taataatcga gtactggttt tattagacca aaaatttcac agtaaactag
     2821 caacattaag taaaaattat ttaattcaaa gtctttttat gacagcatca aaattatttg
     2881 atggatttat agaagatgca agagaaaaaa taatcgcaga acctttcaat gaaaacatat
     2941 tacttaaaca gcatgaagct atatacaatg cagttgtaga aaatgatgta gaacttgcat
     3001 gtgagaaagc aaaggaacat atggatttta taagtaaaaa ttatagaaaa aatgaaaaat
     3061 aaaagtttgt tataaaataa attattcttg aaaatattag ataatggtac tatcatataa
     3121 atgtaaaact ggtatgacca cataccaatt ttatagtcta ttataatggg agattaaaaa
     3181 tggtaaagtt aaaagtaccg tattcaaaac aaggaatgat tttagaaata cctgatgaac
     3241 gttttctcgg tgttttggag tcaaaatcgg ataattatat agaacataag acccaagaag
     3301 agatagttga agaatctatg aataatccta taggctctaa ttctctggaa gaactagtta
     3361 aggggaaaaa gaatgtagtc ataattacaa gtgaccatac aagacctgta ccaagtagaa
     3421 taactatgcc aattatatta agaaggatta ggaagattaa tccagaaatt gatgtgaaga
     3481 ttatagtggc aactggattt cacaggccaa gtacaaggga agagctaatt tataaaatgg
     3541 gagaagaaat agtagataat gaagatatag ttatgcatgt atctactgat gataaatcta
     3601 tgtgcaaagt aggaatatta ccttcaggtg gagatttata tataaacaaa ttagcatatg
     3661 aggcagaact tttaatagca gaaggtttta tagaacctca tttctttgca ggtttttcag
     3721 gtgggagaaa aagcatactt ccaggaatag catctgcaaa gactataatg tacaaccatt
     3781 gtagtgactt tatagacagt gataattcaa gaactgggaa attgaacaat aatcctattc
     3841 atgaggatat ggtatatgca gctaaagttg caaaattagc attcatattg aatgttgtaa
     3901 tagataaaga taaaaaaata attgcttctt ttgctggtga tgtcgaaaaa gcacacacta
     3961 aaggatgtag atttgtaaca gagctatcag aagtaaattc tataaaatct gatattgttg
     4021 taactacaaa tggtggatat ccactggacc aaaatattta tcaatctgtt aaagggatga
     4081 cagcggctga agctacctgc aaagatggtg gagtaataat aatgattgca gcatgtaatg
     4141 atggtcatgg tggtcaatct ttttatgaaa atgtagcaaa cgcagattct ccaaaacaac
     4201 ttttagataa aattagaagt gttgatagag tagatacggt tccagaccaa tgggaatttc
     4261 agatactagc gagaatatta tctaaatata ctgtgataat ggtaacagat aagtgtaatc
     4321 ctgaaatgat aaaaaaaatg catatgaaac atgcatttaa ttttaaagaa gctttagaaa
     4381 aggcaactag tatagttggt aaggatagca aggtagtagt tatacctgat ggagtatcag
     4441 ttatagttaa ataaaaaagg agtgtacttt atggaaattt tgacatgtat taaacaagtt
     4501 cctggaacga cttcagtaga agttgatgaa actacaggag tactaaaaag agatggagta
     4561 gattcaaaaa tgaatcccta tgacctatat gctttagaaa cagctcttag aataaagcaa
     4621 gagaagaaag ctaatctaaa agtacttagc atgggtcctc ctcaggcaaa gaaagtaatt
     4681 gaagaaagtt ttatgatggg agctgatgaa ggagcattaa taagtgatag gagatttgga
     4741 ggggcagatg tattggctac ttcctatacc atatcacagg gtattaagaa gatgggaaag
     4801 gtagacctaa taatttgtgg aaaacaaact actgatggtg atacagcaca ggtaggacct
     4861 gaagtagctg agtttttaga tatacctcat gttactaatg ttacaaaatt aattgaagct
     4921 aaagatgaaa gtatagttgt tgaaatagat atgccaaatg atttacaagt atgtgaaatt
     4981 gaatatccat gtttaataac tgtagaaaaa gatatttttc aaccaagact accttcattt
     5041 aaattaaaac ttgatactaa agatagagaa atacctgtat atagcttaga tgactttgaa
     5101 gataaagatg aaaataacta tggactcaat ggttctccta ctcaagttgt aagaatattt
     5161 cctccaaagc cgaatacaga taaaaatatt gtaagaggaa atgcagatga actaagttct
     5221 gcacttgtaa ataaattaga ggaattaaag ttggtgtaag ggggattaat aaatgtcaaa
     5281 gattgttgta aatcaagata aaataactga cttaaagaga gtattagaaa tatgtccttt
     5341 tggagctata gaagaaaagt caggtgtagt agaaataagt gctggctgta agatgtgtaa
     5401 attgtgtgtc aaaagtggac caaaaggtgc atttgaattt gtagagagta gtaaagtaca
     5461 aattaataaa gatgaatgga ggggaatcgc agtatatgtt gaacatcata atggaaatat
     5521 acatcctgta acatatgagc ttataggtaa agctcgtgag atggcatcaa aaataaaaca
     5581 acctgtgtat tgtgtatttg ttggaaaaga cataaaagat aaatgtaata atttgatttc
     5641 ttatggtgtg gatgaagtat ttgtatatga tgaagatgaa tttaaagatt ttaggataga
     5701 accatattca aaagcaatag aaaactttat aaataaaata aaaccaacga tagtgttagt
     5761 tggtggaact acattaggta ggtcattagc accaagactt gctgctagat ttagaacagg
     5821 tcttacagcc gattgtacaa tacttgatat tcaatcaaat actgatttag accaaataag
     5881 accagcattt ggtggaaata taatggctca tataaataca cctaataata gaccacaatt
     5941 tgcaactgta agatataaaa tattttcagt accagaaaaa atagagaatg ccacaggaaa
     6001 ggtaacttta tgtaaaatag aaaaaaaaga cctaagttct aaaattaaag tactaagtgt
     6061 aaaagaaaaa aataaagaag ttggtctgga agaagcagaa gtgatagttg tggcaagtag
     6121 agcaataaaa aaacaagaag atatggagat gatgtataag ttagctaata agttaaatgc
     6181 tcaagtggca ggaacaagac ctgtaataga agcaggctgg atagatgcta aaaaacaaat
     6241 aggtcttagc ggtagaactg taaaacctaa actgataata acttgtggtg tatctggagc
     6301 agttcaattt gttgcaggaa tgcagggggc tgactatata gtagcaataa ataaagatga
     6361 taaggcgcct atacttgatg tagcacatct agctttaata ggtgatattt atgatattat
     6421 tcctaaattg attgaaaaaa tagaaaataa caaagatgat aatcaaaaat atatggcatc
     6481 agcagctaaa tagggggata gaaatgtata aattagtcga taaaaaagat atagattttt
     6541 taatagatac ttgtggagaa gaaaatgtat tagttggaag tgatataaat gaagattttt
     6601 cacatgatga acttggaggg attgagaagt accctgaagt tttagtaaat gtgcttgaaa
     6661 ctgaacaagt atctaaaata atgaaatacg catataaaaa taatatacca gtcacaccaa
     6721 gaggtcaagg tactggtctt gtaggtgctg ctgttgccat aaatggtgga ataatgatta
     6781 atctatgtaa aatgaataaa atacttgagg ttgattatga aaatttaaca ttgacagtag
     6841 aaccaggtgt tttgttgatg acaataggtc aatatgttca agatagagat ttattttacc
     6901 cacctgaccc aggtgaaaaa agtgctacaa ttgcaggtaa tataaacact aatgcaggtg
     6961 gtatgagggc agtaaaatat ggcgttacaa gagattatgt aagaggtctt gaagttgtac
     7021 taccaaatgg agaagttata aatgtaggtg gaaaggtggt taaaaatagt tctggatata
     7081 gtataaagga cttattagta ggttcagaag gtactttagg aatagttact aaagctatat
     7141 taaaattgct tcctttacca aagaaaagta taagtctact tgtaccattt ccagatttat
     7201 caatggcaat agaaactgtt cctaaaataa taaaatcaaa atcaatacca acagctatag
     7261 aatttatgga aagagatgta atactggcag cagaagagtt tttagggaaa aagtttccag
     7321 ataatacatc agatgcttat cttttactta catttgatgg aaatagtact gaagatatag
     7381 aaaaagaata tgaaaaagta gctaatttat gtctagaaaa tggtgcatta gatgtattta
     7441 tatcagatac acaagaaaga aatgattcta tatggtcagc cagaggtgca tttttagagg
     7501 ctataaaggc atctacgaca caaatggatg aatgtgatgt tgttgttcca agagataaga
     7561 tagcagagtt tataagatac actcacgagc ttcaagacaa attaaaaata agaataaaaa
     7621 gttttggtca tgcaggagat ggaaatctac acatatatat tttaaaagat ggtatggatg
     7681 ataatacatg gaaaataaga ttaaaagaaa cttttgacta tatgtataaa aaatctagag
     7741 aacttagtgg tcaagtttct ggtgagcatg gtataggata tgctaaaaaa gagtacttac
     7801 atgaatcaaa ttcagatgct tatatgatgt taattaaaaa tataaagtta gcttttgacc
     7861 ctaaaaatat tttaaaccca ggaaaaatat attagaaata ttaatttaat aatgtttgta
     7921 aaacaaaaaa tattaaatta gatttaaatg taatatttta aaccttacct ttaaatttga
     7981 agctaaattt taaaagtttt aggatggcaa tatttactta aatttagaaa gtaacataat
     8041 atttaagttg atgttgtcat cctaaaaaca tgtgtacata gataaagtga tattattgta
     8101 tcttatataa taaagagtta ataaaatagt ttttatgaat atagttattt taaaaaatta
     8161 ttttacatat atcggatgag tataaaaatc catagggcct tttactaaat ctttattgtt
     8221 tttataatat actgaattat atatatttgt agattttata tcataactta tgagtgtttt
     8281 aaaaatcgta tcatctaatg aaaatgatat atttgaaaat ttgtttatta tttttatttc
     8341 agatttattt tttatctctt ttagtacttc tcttcctttg tcattaaaag caagtattct
     8401 tatataaggt atagtatcta tattttttat aagttgcatg tcgctttttt ttatacccaa
     8461 caatatatta tttagagttc gtttgatttt ggttaaagtg tatcgcttgg atttaatcga
     8521 taattgtagt tcatgtaaaa attcagacgt aaaaatatcc ctgtagattc tgttttctat
     8581 gccttcattt acttcaaaat aatcttttaa agtgtccata tctctaagta taattgtttt
     8641 taaagtatca aagaacaagt catcaaacat aggtaaaaaa ccattatcta ttttttcatt
     8701 taagatatta taagtttttt ctggaataac attttttaaa tgaaaaaagt ttatattttc
     8761 tttaagtgaa gttcttatag cagtagcaga acaaatatta ttcttaattt cttcagaatt
     8821 atattctgat tgaaccctgg atatagtaaa tggttttata ctactgttta aatggatgag
     8881 gttttttata tattctatac ccagtatgtt gtttgatgaa tttaatatct ttgataattg
     8941 ttcctttgaa taattttgta taaaattatt ttttttgtct tgatgtatat aatcaaaaag
     9001 tgcattagct ctagccgttg gaaataacag accatcatct aaatattttt ttaagagtaa
     9061 cttaaattca gttggttcat ttgctaaaat ttcagctata ttataaagaa tttctgtatt
     9121 tccttcttca ctgccaaaac atatagaatt tatgcagttt agtgaattta aaattgttat
     9181 agcaccatga gaaaaaattt cagcactttg gcaagcaaat aaagttggta attctataac
     9241 taagtcgaca ccattttcga cagcaatttt tgctctagtg tatttatcaa ataaagctgg
     9301 ttcgcctctt tgtagaaaat ttccactcat tattgctaca gtatgtgttg catttgtctt
     9361 ttctatagat ttagataaat gatatatgtg tccattatga aatgggttat attcaactat
     9421 taatccaagt atgttcataa aatactcctt ttatctttag ttattttata tatatcacaa
     9481 tttattataa tatgaataaa atttgaaatt ttaattataa aattaaaaaa atttataaaa
     9541 atgtagaaat gttacctaca taaaaaatta gttaaaagtt attaaaataa ctttattagg
     9601 tctttatatt atattattat aacaaatggg aaagaataat ctacattaag ttaacttgtt
     9661 aatatattat tagagaaata cacaatttac catactagaa atatgatata atataaaaaa
     9721 aaagtatata cttttatatt aaaaggtgat atattattta tgaaaatcgt ttaatgtata
     9781 caataatata tctatgacaa aataataaaa gatagagttt tatttagaca tttattacaa
     9841 atcaaaattt attacatatt atttataaaa aaatctaagg aggaaataat aatgaaaata
     9901 ttagtattaa actgtgggag ttcttcatta aaatatcaat taatagatat gaataatgaa
     9961 gaagttttat gtataggatt ggttgagaga ataggaatag aaggttctat attaaaacat
    10021 gaaaaagcag gtagagatga taaatacgtt gttgaacagc caatgaagga ccacaaagat
    10081 gcaatagcat tagttttaga ggctgttgct catccggaat ttggtgcagt taaagagatg
    10141 aaagaaatag atgcagtagg acatagagtt gtacatgctg gagaaaaatt tgcaacttca
    10201 gtagtaataa ctccagaagt agaagatgct ttaaaagaat gtatagattt agctccactt
    10261 cataatccag caaacataat gggaatagat gcttgtaaag ctatacttcc agatgtacca
    10321 atggtagctg tatttgatac tgctttccat caaacaatgc ctaaatcttc atacttatat
    10381 ggtttacctc atgagttata tactaaatat ggggtaagaa gatatggatt ccatggaact
    10441 tctcacaatt atgtatctca aagagcagca gagatattag gaaaagatat aaaagattta
    10501 aaaatagtaa cttgccactt aggaaatggt gcttctatag ctgctgttga tggtggaaaa
    10561 tgtgtagata catctatggg attcactcca ttagaagggt taataatggg aactagatgt
    10621 ggagatatag acccagctat attacctttc ttaatgagaa aagaaggttt agatgctgat
    10681 ggattggata aattaatgaa taaagaatct ggagtatatg gaatgactgg aatttctagt
    10741 gacttcagag atatagaaga tgcagctaaa aatggagacg aaagagctca agcgacatta
    10801 gaagcttatg ttaaaaaagt acaaaaatat ataggagctt atgctgctga aatgaatgga
    10861 ttggatgtcg tagtatttac tgcaggcgtt ggtgaaaatg gaaaagctat aagagcagat
    10921 attgcttcta acatggagtt tttaggaatg aaattagata aagaagctaa tgatgttaga
    10981 ggaaaggaaa cagtaatatc tactgctgat tctaaagtaa aaatgctttt aataccaact
    11041 aatgaagaat taatgatagc tagagataca ttaagattgg taaaataatt aaattaaacc
    11101 tatgcctatc aagtaaaaat acttgacaaa caaatgctag atttgtataa taaatttggt
    11161 gatatgttga ggtgaactta atgaaagtta gcatagaaaa aataaataga aaagaaactg
    11221 acaaaataga cttgaatttt tgtgaaaaaa ttgatactat tagttattgt gatgagattt
    11281 acaaattagt atcacctgta aatttaaaag ggaaagtgtc aaagactaat aaaggtttgt
    11341 atctagatat agatgtcaat tttacaattg ttgacaattg ttcaagatgt cttaaagaag
    11401 tagaaatacc actagagtat tctatacaag gttttttagt aaaagaagaa gattatgatg
    11461 aagatgagtt tgaggaattt gatcctttta tattcgatgg tgaagaaatc gaccttattg
    11521 atataataga acagacatta gattttaatg tacctcataa agttctttgt agtgaaaact
    11581 gtaaaggtct ttgtcaagta tgcggggcaa acttaaatga agaagagtgt tcttgcagtg
    11641 aaattacaaa cgatgaggaa tacatagatc ctcgttttgc taaattaaaa gatttattta
    11701 attaaatttt gactaaggag gtggcgtaaa tggcagtacc aaagcgtaaa acgtctaaat
    11761 caaacacaaa aatgagaaga gcagctaact caaagatgga agcaacagga tttgtaagtt
    11821 gcccacaatg tcatgagcca aaattaccac atagagtgtg tccagactgt ggatattata
    11881 aaggtaaaga agttgtgtct aagtaatagc ttctaaaaag catgtagagt atactctaca
    11941 tgtttttttt gtacatataa aaaaagaaga tgatttacta gagaaaataa gtggatttta
    12001 tgcatattaa aaaatagaaa aataataata taagtattga aaatttttgt gtgttactat
    12061 atactactat tagtagctgg tactaattac agctataaaa tttggaggga acatgaaaaa
    12121 gaaaagtaaa gcccaaagac aaaaagagct tatagatatg ctaaagacag atccttttta
    12181 tactgatgaa gaattatcaa gtctgtttga tgtaagtata cagaccataa gattagatag
    12241 aatgagtcta aacataccag agctaagaga aagagtcaaa tcaatagcag aaactcagag
    12301 ttcaaaagta aagactttag gagtaaagga aattacagga gaaatcattg atttatctgt
    12361 agggcgatta ggtatatcta tgttagaagt tacacaggac atgatttact ctaagactaa
    12421 tactttaaaa gatacgtata ttttttctct agcagattca ttggcaatgg ctataataga
    12481 tgcacctaag gttattatga gagttgcaaa tgtaaagagt tttaagttga tagaacaaca
    12541 agataggctt atagcaaaag cagaagtgta tagaaatata gataaaaagc attatgtaaa
    12601 agttgttatc aataataaag cccaagagca aatatttaga ggaaagttta tctttgaaga
    12661 attagattag gagggaaatt atgaaaatag taattgatgg aatgggtgga gataatgcac
    12721 ctaaatctaa tgtagaaggc gcagtaaatg ctataaaaga atatcaagtt gatttaataa
    12781 tcacaggaga taaagattta cttgaaaagg aattttcaaa ttatgaattt gatagaaata
    12841 aattagagat agttcatact actgagatta tagaaaatga agataaacca gtaaaggcta
    12901 taagaagtaa aaaagattcg tctatggtag tatcattaaa tcttgtaaaa gaaggaaagg
    12961 cagatgctat aatatcagct ggaaatacag gagccttact tgctggtgga ttatttgttg
    13021 ttggtagaat aaaaggaatt gatagacctt gcttatgttc agcaatacca aatgtaaagc
    13081 gaggaatgac acttatagca gactgtggcg caaatgcaga ttgtaaacct aagaatctag
    13141 ttgaatttgc agctatgagt aatatttatt caagaaaagt tttaggtctt gaaaatccta
    13201 aggtagcatt agcaaatgtt ggactagaag aaggtaaagg caatgattta gtcaaaaggt
    13261 catatgaaga aattaaaaaa ttagatttaa attttatagg taatgtagaa gctagagaag
    13321 ttataaatgc atacacagat ataatcatat gtgatggatt tacaggtaat atattactta
    13381 aatctgctga aggagtagct ctttcagtta tgagtcttat aaaggaaact tttatggcca
    13441 gtacaaaaag taaaatagga gcattactta taaaggatga tttaagaaaa ttaaaaagtt
    13501 ttatagatta ttctgagtat gggggagctc cacttttagg tctaaatgga ggcgttataa
    13561 aagctcatgg tagttctgat gctaaagcta taaaaaatgc tataaatcaa ggaataaaat
    13621 tttcaaaggg aaaagtcgtt gaagatatta agcaatttat aagcaaatac aataaagaaa
    13681 ataaaaataa cgaagatgaa taaactcgtt ttggaggttt aatatataat gaatacaaag
    13741 gctggaatat taggagtagg aagttatcta cctgagcaat catatgataa ttttcacttc
    13801 gagaaaataa tggatacatc agatgagtgg ataagtacta ggactggtat taaggaaaga
    13861 agatttgcca aagagtctga agcaacttct gacttagcat ctaaagctgc cttaaaagct
    13921 atagaatgtg caaagttaaa cgtagaggat atagagctaa ttatattagc aacaattact
    13981 cctgatatgt ctttgccttc aactgcatgt atagtacaag atgcaatagg agcagtaaat
    14041 gctactgcat ttgatatatc agcagcttgt tctggatttg tatatggagt aactattgca
    14101 aagcaatttg tggaaacagg ttgctataaa aatgtgcttg taataggggc agaaacttgt
    14161 tctaagtttt taaattatga tgatagaaca acagcagtat tatttggaga tggagctgga
    14221 gcagctgtta ttggtcctgt aagtgaaggt ggaatactgt ctacacatat gggctctgat
    14281 ggtaaaggaa aagattgttt aaaagtacct gctggaggtt caagacttaa ggcaagtaaa
    14341 gagactgtag aagctaatct acatacaata gaaatggcag gtagtgatgt atttaaattt
    14401 gcagttagaa agatggcaga aacttcttta agagctcttg aaaaagcaaa tttaaataca
    14461 actgatatag actatttagt gccacatcag gctaatataa gaatcataca agcttcctct
    14521 aagagattag agcttgatat gaaaaaagtt tatgtgaata tagacaaata tggaaatatg
    14581 tctgctgcat ctatcccagt agcgttagat gaagcttata gagaaggtaa aattaaaaag
    14641 ggtgataatg tagttttagt tggctttggt ggaggtctaa cttggggggc ttcagttgtc
    14701 aagtggactt tatagtataa aatatctaaa atgctttaat taacaaaatt aaacttatgc
    14761 acaaaataaa gttgattaat aataaaaagt ttacttttat gatttcaagt gaaggagaat
    14821 tgtatgaata aaatttgcaa aatattaaat ataaaatacc cagttatcca agggggaatg
    14881 gcatgggtag ctactgcatc attagcaagt gctgtatcta atgcaggagg gcttggcata
    14941 atagcagcag gaaacgcacc aaaagaagct ataaagaaag aaattgttga gtgtaaaaaa
    15001 ttaactgata agccttttgg agtaaatgta atgcttatgt caccatttgt tgatgatata
    15061 attgatttaa ttatagaaga aaaagttcaa gttattacta ctggtgctgg aaatcctgca
    15121 aagtatatgg atagattaaa ggaagctgga acaaaggtta ttcctgtagt acctacaata
    15181 gctttggcgc aaagaatgga aaaactagga gctacagcag taatagcaga aggtactgaa
    15241 ggtggaggac atataggaga acttactact atggtcttag ttccacaagt tgctgatgct
    15301 gtaaacatac ctgtaatagc tgctggagga attgtagatg gtagaggaat tgctgcatca
    15361 tttgcattag gtgctagtgc agttcaagta ggaactagat ttatttgtag tgaagagtgt
    15421 tctgtacatt caaactataa aaacctagta ctaaaagcaa aagatagaga tgcaattgta
    15481 acaggaagaa gtactggtca tccagtaaga acattaaaaa ataaattatc aaaagaattt
    15541 ttaaagatgg aacaaaatgg agctactcct gaagaattag ataaaaaagg tacaggagct
    15601 ttaagatttg caacagtaga tggagacata gaaaaaggtt catttatggc aggtcaaagt
    15661 gctgctatgg taaaagaaat aacaccttgt aaggaaatta tagaagctat ggtaaatcaa
    15721 gcaagagaga ttatgccagc aatagaactg taaatcagga ggagcaagat gggaaaagtt
    15781 gcactagtat tccctggtca aggagctcaa tatgtaggta tggccaagga tttatatgaa
    15841 aataatactg ttgctaaaag tgtaatagat gaggccagtg atgctttaaa catggactta
    15901 aaaaacctta tgtttaatgg aaatgaagaa gagctttcta aaactgaaaa cactcaacct
    15961 gctatggtta cacatagtgt agcagtatta aaagcagtac aagctgaaat agatttaaaa
    16021 tataatgcat gcttaggatt atctttagga gagtatagtg ctttggttgc agcagatgca
    16081 attgacttta aagatgctgt atgtttagtt aaaaaaagag ggaagtttat gcaggaaaca
    16141 gttccaacag gtgttggagc tatggcagca attttaggtc tagatagaaa tgcacttgaa
    16201 agtgtggtta aagatataca gggtggtatt gtagaagtag caaattacaa ttctcctgga
    16261 caaatagtta tatctggtga aaatgagaag atagaagaag caatagtgaa atgtaaagaa
    16321 gcaggagcaa aaagggcagt taaattaaat gtaagtggac cttttcactc atcaatgtta
    16381 aaagaagctg gagtaaaact ggctagtgaa ttagaaaaag ttaacatgac taaacctaaa
    16441 gttgatgtgg tggcaaatgt aaatgcagat tactataaaa atgaaggtaa agaactgctt
    16501 ataaaacaag taagctcatc tgttctttgg gaagattcta tagaaaggct tttaaatgac
    16561 ggatatgata cttttataga aatgggacct ggaaaaactt taaaagcatt tattaagaag
    16621 atagcttcaa ataaaaaagc aaatgtcaat atttataata tagatggtat agattctctt
    16681 aatgaatttg tacaaaatta tagaaatgga gagatataat gataaatctt acaggacaag
    16741 tagcagttgt tactggtggc tctaggggta taggaaaaga aatagcaaaa aaactagcat
    16801 cttttggagc tgatgtagta atcaattata cttctaaaga agatgaggca ctaaaaacta
    16861 aaaatgaaat agaaaatatg ggggtaaagt gtacttctat aaaatgtgat gtgtctaaat
    16921 ttgatgaagt gaaccaaatg atagattctg ttgtaagcga atttggaaaa attgatatat
    16981 tggttaataa tgcaggcata actaaagatg gcttgcttat gagaatgaaa gaagaagatt
    17041 ttgatagagt tatagatata aacttaaaag gtgtctttaa ttgtacaaaa gcagttacta
    17101 aacctatgat gaaaaagaag tatggaagaa taataaatat gacttcagta gttggaatta
    17161 tggggaatgc agggcaaact aattattgtg catcaaaagc aggtgtaatt ggatttacaa
    17221 aagcttctgc aagagaatta gcatcaagaa acataaatat aaatgcagta gcacctgggt
    17281 ttatagaaac agatatgact aaagtactaa gtgatgatgt aaaagaatca acactagcaa
    17341 acataccaaa aaaatcttat ggtaaaccag aagatgtagc caatgctgta gcatttttag
    17401 ttagtgacat ggcaagttat ataacaggac aagtaataaa tgtagatggt ggaatggtaa
    17461 tgcaataata aaaaataata atttaaaaag tagagaaatc tattaaaata aaaattttag
    17521 gaggtacaca atgtttaata aagttgtaga aataatcata gagcaattag gagtagaaga
    17581 taaagaaata actatggaga catcattaat gaaggattta gaagcagatt cattagatgc
    17641 agtagaaata ataatggcac ttgaagatga atttggtata gaaataccag atacagaagc
    17701 agaaaacttt aaatctatag gagatatagt taagtatata gaagctaata aatagtcgag
    17761 ctttaaaaaa gtggaggtat ccgatgaata aaagagtagt aataacaggg cttggatgtg
    17821 ttacaccgtt gggaacggga agagaagagt tctggagtaa tataaaagct ggagtatctg
    17881 gtatagataa aataactaat tttgatgcta gtacgtatca aactcagata gcaggagaag
    17941 ttaagaattt tcatccagaa gaatatataa gtaaaaaaga attgaagaga ttagataaat
    18001 ttgctcaatt tgcagtagta tctgcaaaat tagcagttga agatgcaaat cttgatttag
    18061 ataaagttga tagagaacgt tttggtgtta taataggttc tggaataggt ggagttgaag
    18121 ctatagaaac tcaacataaa attcttttgg aaaaaggaaa taaaagagta agttcacttt
    18181 ttgtacctat gatgatagga aatatggcag ctggtcaagt ttcaatattt ttaggagcaa
    18241 aaggacctaa cactaatgta tgtacagctt gtgcatcagg aactcattca ataggtgatg
    18301 cttttaaggt aattcaaaga ggtgatgcag acattatggt agcaggaggt tctgaagctg
    18361 ctgtaacagg tcttgctttt gctggattct gtaatatgaa ggctatgtca actagaaatg
    18421 atgaccctaa aactgcatca agaccatttg ataaagacag agatggtttt gtaatgggag
    18481 aaggagcagg tattgttatt ttagaagact tagaacatgc tttagcaaga ggtgctaaaa
    18541 tatatgcaga agtagttgga tatggattaa ctgctgatgc ataccatatg acaactccag
    18601 ctgaaaatgg agagggagca gctaggtcta tgaatatggc tttgaaagat ggaaatgttc
    18661 cactagagga agtcgattat ataaatgctc atgggacttc aacttattac aatgatttat
    18721 acgaaactat ggctataaaa actgtatttg gggaaaaggc atatgattta tgtgtgtcat
    18781 caacaaaatc tatgacgggt cacttattag gagcatcagg tgcaatagaa gctgttgttt
    18841 gtgctatgag tatagaagat agttttgttc ctccaactat aaacatacaa gaagttggcg
    18901 aagatttgga tttagattat gttccaaatc aaggtaaaga aaaaaatatc agatatgcac
    18961 tttcaaattc tttaggattt ggtgggcata atgctacaat agtactaaaa aaatatgtat
    19021 aaataaatat taaataaaaa aacaagtact tattttaact gtataaattt atattggatt
    19081 aaatttaaag tctcctaaat tattttagga gactttttta ttaagattta agtctagcat
    19141 atgttgtaaa tcattttgat atattgtatt attatttatt aagaggttat ttatatatgc
    19201 aagttttcat tctacaatat acaaaaggag gactaaaatt ttgaatttta gactaccaaa
    19261 gaaaaggtta cctaaaaaga taatttttat atgtataata cttattagta ttgttttatt
    19321 tataaagtat gaccaaaatg gattgaaaga aagagaaatt ataagtaagg gaaaatatat
    19381 gtctgaaagt cttgtggcag ataagtcata tgatgaagca aaaaaagtgg ttaagacttc
    19441 ttttgagtct attccagcaa aaaaatacaa taaagtatat aatgaagttt gggatatgtt
    19501 aaagactatt tcatatgttc cagatggaac gaagatagtc attgattctt tagaaaagat
    19561 gagagaatca gatgcaaaat tatctgatga gtgcaggttt aatatagaaa aaagattagc
    19621 atcagtgtat ataatggata ataattattc taaagcagta gatttaactg taaagtctat
    19681 aaaattggct gagaaattgg ggaataatta tgaaaaagca agactagatg ttgatttggc
    19741 gagcatactt ttaaaggtag gtagttataa aacaggagaa aaattaataa aggattcttt
    19801 taaaatagat attgatgaag gtgaaaaaaa tggaatggtc agattatatg ctcttataaa
    19861 tctggcagaa atatatgtag aggtaggaga atatgataag gctattgaaa taagtagcag
    19921 agtaaaagat tataaaaaat ttgtaaatac agataattac aatgattttg agataatggc
    19981 ttctataatg caggcaaatg cttatataga aaaaaataat atacaagagg caaagaatct
    20041 tttagaaaaa gctgattata ttataaaatc agatagaaca gttcatatat tagataaaga
    20101 tatgtactat tatatggcaa tgggaaattt agaatataaa aatgaaaatt atgattctgc
    20161 aatagataat tataaaaaaa gtttagatat atcaaccaaa agaaagctaa cacaagaaaa
    20221 aataaaaaat ttaaataaga tattaaatat atatgaaaaa caaggaaatg tagaatcttt
    20281 aaatgagtat caaaagatgt taataaatga atataaagag aacaaaagta taagagattc
    20341 agagactagt ttctatataa ttgataaagt atcaaatgaa agtgagctat ttgaaaaaac
    20401 taaaaaagaa ataaaatatt ataaaatatt atttacagta attttaattg ctgtattagg
    20461 gtctactttt ttatacaaca gattgaagta ttttaaagca caaaatttac atgatgggct
    20521 tacaaatgtt tataatagaa aaagctttga tattatgtat gaaaagtata gagctaaaga
    20581 tgataatttt gcacttgtaa tgatagatat tgataatttc aaactcataa atgataatta
    20641 tggacataaa tttggtgatg ttgttataaa agggataaca aatactattt gtgttatgtt
    20701 ggaacaagat gataaagttt ttaggtatgg aggagaagaa ttttctgtat tgataagaaa
    20761 taaatctggt gaagaagtag cagatatagt tgacaatata agagttgcaa ttgcaaataa
    20821 aaaatggaat gaagatgtaa ctgtgactat aagtgcaggt gtagctcata tttcggatag
    20881 caaagatgta ctagaagaag ctgataaaaa tttatataaa gcaaaacaac ttggtaggaa
    20941 taaagtagta tataagtaac tgtatactat ataaataaga ggtataagta tgaaacaatc
    21001 aatagctata gtaactgata aattaactaa actatcatct ttcttagaag aaaatatacg
    21061 tttggtttta ggaaattata ttgacataaa ccattattat ctagaagttt tgaaagagga
    21121 tgataaaata caaggtgatt ttgtactagt aatgaatgat gatagattaa acagtatgag
    21181 aaaacattta gtacaagata gtaagattat agtgataagg agaaccctta aagaaaatga
    21241 aatatatgga ttattttcaa taccaaatgg aactgaagta ttggttgtaa atgatacaaa
    21301 ggaaactacc ttagaaacaa taagtttatt ttataaaata ggagtaaaaa atttaaagtt
    21361 tactccatat attgaaggaa aaatttataa aaatatagac atagctataa cgcctggagt
    21421 aaaggaaaaa gtaccaagtt atataaataa agtaatcgac cttggtaata gatatatcga
    21481 tatatctacc tttatagaaa taataaatgg tcttagaatt gatgttaaag aaatacgtaa
    21541 aaatttaatg gcatattctg agagtttaat aagtttagat aatggagtta aagataatta
    21601 tagacaactg tttttaaaaa tagaagaaca agatgttata ttgaatctat caaaagatgg
    21661 tataatattt acttcagcag atggtgtaat aaacacattt aataaaaaag ctttaaaaat
    21721 tttaggtata aatgaaaatc cgcaaggtaa aaaaatagaa gatatcattt ctaaagattt
    21781 aaatatttta cttggagaca aagaaataat tgatgaagtt gtatttacaa ataaaaaata
    21841 tataaatgta aataaaaaaa gtattttttc tatggggaat aaagctggta tatattataa
    21901 cttgcaagaa ataacgtata taaaaaaatt agaacaaaac cttactaata aattaagaga
    21961 acaagggcag atagcaagat atacgtttaa agatataaaa actaaaaata aaaatatgat
    22021 aaagtgtata gaattggcta aaaaaatatc taaatcagac ctttctgtgc taataatagg
    22081 agaaagtggt actggtaaag aattattatc gcaatcaatt cataatgctt ctaataggag
    22141 taatcaaccg tttatagcag ttaattgtgc agcagttcca gatagtctat tagaaagtca
    22201 actgtttgga tatgaaaaag gctcttttac aggagcttta agagaaggga aaaaggggtt
    22261 atttgaactt gctaataatg gaactatatt tctggatgaa attggagata tgcctccatt
    22321 gctacagaca aagttactaa gggtattaca agaaaagcag gttatgccta taggttccca
    22381 taatgttata aatatagatg ttagggtcat agcagcaaca aacaaaaact taccaaagat
    22441 gattagagaa ggaaaattta gagaagactt gtattataga ttaaatgtat taccaataga
    22501 tgtgccatca ttgaggaata gaaaagaaga tataataaca cttatgaact tctttttgaa
    22561 aaataatata agactttcac cagaagttat aactatttta gaaaaatata attggccagg
    22621 aaatataaga gagttacaaa atgttgcttc ttacgtaagt ctaatgtgtg atagtatggt
    22681 ttttagggat gacttaccac catatataag tgtagaattt gctaataatt atgaagatgg
    22741 atttggctat acaaaaaaag aatttaagga aatactaagg attctatatg aaagaagaga
    22801 gtcgtcttct ggagttggaa gagggtttat aattgaaaag cttatacagg aagggattga
    22861 gatttctgaa agcaaagtta gaaaggtgct atcttattta agtgaatgtg gatatatatc
    22921 atctaaatct ggtagaggtg gaagtcaaat aacctcagag ggtataaaac tttataataa
    22981 tataaattaa gttaagtata ttgatattga aattacatat tttgaattta gatatttaaa
    23041 taataaactt ttatatttat agaaattaat ttatattttg tcacaaagaa tattataatt
    23101 tataattaaa tattaatagg tttttaaaat aggattagat aggtaatagg ttaagttaaa
    23161 ataacccatt acctattttt atttgtagga aaagtgtcta tgaaattaaa agtaaaaaat
    23221 accactaata aaacgattga aaacactaga taaataataa ataaaaataa ttataaattt
    23281 tggcacatat attgctaatc atagaaataa gatgacaaaa taatagggag gtttaataat
    23341 gaaatacaaa acaacaggga cttgtgctac agaaattgaa tttgaggtaa aagaaaataa
    23401 agttacaaat gtaaacttta taggtggatg tgatggaaat ttaaaaggga taaaagtatt
    23461 agttgaaggc atgaatatag aagatgtaat acaaaaatta aaaggaatag agtgtaaaac
    23521 aaagccaacg tcttgtccag accaactgtc tctagcattg gaaaattata tgaatataaa
    23581 ataatacata aaacaatcga ttagttagga gtttaattat gaaaccaata atagggattt
    23641 taggaaattt aataataatg gaaaatggta tgtttccagg tcttgaaagg tcatatgtaa
    23701 ataatgatta tataaatgca gtactaaaag gtggaggtag tccagtaatc attcctgtaa
    23761 atacagataa agaagttata aaaaaacaga ttgaaatggt tgatggagtc ttaatatctg
    23821 gaggatggga tgttaatcct caactgtatg gagaagaaac aagagaagaa acaacattta
    23881 tatatccaga agtggatgaa tttgatttaa ttgcaataaa tatagcatta gggcttcaaa
    23941 aacctatatt aggtatatgt aggggactac aaattttaaa tgtaagttta ggaggaactt
    24001 tatatcaaga taataatctt aaagaaggaa gttatataaa acacacacaa tcttccaaaa
    24061 gacatgtagc tactcataaa gttgatgtta aagaaggaag tattttagaa ggaatattgg
    24121 gaaaacaatt acttacaaat agttatcatc atcaaagtgt caaccagctt ggaaaaggat
    24181 taaaagccat agcatactca aaagatggta taatagaagc tattgagaaa gaggatgaaa
    24241 aatttgtagt aggagtacag tggcatccag aaatgatggt agactactgt aataaaatgg
    24301 aaaaactatt taaatatttt ataaatattt gttcaaattt ataatatacc ttggagggga
    24361 ttgaacatga acgaaaagaa taaaatggga ttgataagta ttatactttt aggcattaat
    24421 gcagtagtag gagctggagt ttttctatta ccaggagatg caatgaaggc atttggtgta
    24481 gcaagtatat ttgtatatat gtttgatatg ttattagttt tatctatggc attctgtttt
    24541 gctgaggttg caggtaaatt caataaaaat ggtgctgcct atgtatatac caaagaagct
    24601 tttggagatt tttgtggttt tgaagtaggt cttatgaaat gggtaatagg atgtatctct
    24661 tggggagctt taatagtagg ttttcctaca tccttatcag cagtatgggc tccagcaggt
    24721 gaaccacata tacaaaaaat tataattgta gccatgatag ttggacttac aattataaat
    24781 ttattaggtg tatctctatc aaaaattgta caaaatgtta taactgtagg taagctaatt
    24841 ccattaatat tatttatagg aataggtata ttttttataa aaggagttaa ttttactact
    24901 tcaacaatgg tgcctccagg agctggagct acagagtttg gagcagcagc acttttaatg
    24961 ttttattcat ttacaggatt tgaatctata gcagttgcag cagaagatat ggaaaatcca
    25021 caaaaaaata taccaatagc aattataagt gtaatagtaa ttgcatcaat aatatatata
    25081 ttaaatcaag ttgtatgtgt tggtatacta ggagattcat tatctagtac atctacacca
    25141 gtggcagacg ctgcaagagt atgctttgga aatatgggag ctggactggt tacatttgga
    25201 acattggttt ctgttggtgg aatttgtatg tgtggcgctt ttgtaaatcc aagaagttgt
    25261 gtagctttag ctgatgataa aatgttaccg agaatatttg caagaaaaga taaaaaaggt
    25321 acaccatatg tagcaataat agctactatg ttaattacta tcccaatagc tctatctgga
    25381 agttttgctg aattagcagc aataagtgca gttgctagat ttatacaata tataccaaca
    25441 tctttatcag tattagtgtt tagaaagaaa agaccagaat tagtaggtac ttttaaaact
    25501 ccttttggag cagtgattcc tctaattgct gtatgtgttg gaacatggtt attattccaa
    25561 gcatctatgc atcaattaat tatgggatta ggagctttgg caattggagt accattatac
    25621 tttataatga agtcttataa tagaaaagtt tatggtgaaa atttaaagaa aatagtttaa
    25681 ttatgttata acagtttatt gatttaaagt tagatattgg cagaaaatta aatacaaata
    25741 agtatctata aggctatatt aaaatattat tgatttttta ttttaatata gccttatatt
    25801 gttaatcagt aagtgaaaaa tattgtaaca acaaaagaac aatagtatta tgtttataga
    25861 caaaaactta ttaggaggta aagttatgcc aacaataaca ttaaaaaatg gtgtagatgt
    25921 acttattaga gaaggagtaa gagaagatgc tcaaaacatt atagattttt ataatgaagt
    25981 tggtggagaa actcattttc tgtcatttgg aaaggatgaa tataaaatat ctttgaaaga
    26041 acaagaaaat gctatagaat cagcaaaagc atctgataat tcggttaaac tgattgcatt
    26101 tatagatggt gaaatagttg gtattgctac aattgattct aatcaaaagg caaaaggaaa
    26161 gcatgttgga gtattaggaa ttgttgtaaa ggaaaaatat tggggaattg gactgggtaa
    26221 aaggcttatg ttagacctta tagaatggtg taaatcaaat ggaataacca agaagataac
    26281 tcttgtcact aatgaagaaa attataatgc tataggatta tacaaaaaag ttggatttga
    26341 agtagaaagc atactaaaga aagaatgtta ttataatggt gtttacactg atttaatagg
    26401 aatgtcactt ttgttgggaa tataattagt gtacatttta tatcgaaaaa actatattaa
    26461 gtatggtata attaaaaaag aatataactt aatataattt tggaggacga attatgaaaa
    26521 atctgtgtaa aatatcagat gattatttaa acagcaagct taagtattta aagttattat
    26581 caaaacaata tccaagtata tcaaaagcaa gtacagaaat aataaattta gaggctatat
    26641 taaatcttcc aaagggaaca gagcatttta taacagatgt acatggtgaa tatgaaccat
    26701 ttgtacatgt attaaaaaat ggttctggtg taataaagag aaaaatagaa gaattgtttt
    26761 caaatacaat aagagacagc gaaaaaaaaa tgttagcaac tcttgtatat tatcctgaac
    26821 aaaagttaga cctaataatt aagcaagagg aaaatataga tgatttttat agaataaata
    26881 tttataggct tatagaactt tgtaaatatg cttctagcaa atatactagg tctaaagtca
    26941 gaaaactgtt accagaaaat tttaaataca taatagaaga acttttgcat gagcatgtta
    27001 aaagtgaaca taaagaagag tactataaaa gtatagttga aacaatagtt gatataggaa
    27061 tagctaaaga gtttataatt gctatttcta cagttataca aaaattagtt gtagatagac
    27121 ttcatgtaat aggagatatt tatgatagag gtccaagacc agatataatt gtcgataagc
    27181 ttattgaaca tcattgtgtg gacattcaat ggggaaatca tgatatctta tggatgggtg
    27241 cagcatcagg tgaaaaaact tgtatagcaa atgcacttag gatatcagct agatatgcaa
    27301 atttagacat tgtagaagac atatatggaa taaatctatt gcctttagcc acttttgcga
    27361 tagagatgta taaagatgac ccttgtaaag aatttattcc aaagattaat gaccaaagtg
    27421 taacaacaac agaaaagtct ttaatggcaa aaatgcacaa agcaataagt ataattcaat
    27481 ttaagcttga aggtgaagtt ataagacgaa gacctgagtt tgagatggaa catagacttt
    27541 tacttaatat gataaactat gatgaaggta cgattacttt aaaaggaaaa acatataaat
    27601 taaaagatac ttatttgcct acaatagaca aaaaagaccc atataaatta actatagaag
    27661 aaaaaaatgt tattgataaa ttagtatctt catttagagg aagtgaaaaa ttacaaaaac
    27721 atgtttcatt tctgttctct aaaggaagta tatatttaaa agctaactca aatttactga
    27781 ttcatggatg cgtaccatta aatgaagatg gaagctttat gtctatgaat ataatgggca
    27841 aagagtataa aggtaaagcc cttatggata gaatggagtc tctagcaaga gaaggatttt
    27901 tctttaaaga taaggcagaa gaaaaactgt atggcatgga tattatgtgg tatttatgga
    27961 ctgggaaatg ttcatcatta tttggtaaag atgatatgac tacatttgaa agatacttta
    28021 tagcggaaaa agagactcat aaagaaaata agaatccata ttttaaactt agagaaaatg
    28081 agatggcttg taaaaaatta tttgaagaat ttgatttaga acttgatgaa tctcatataa
    28141 taaatggtca tgttcctgta gagagtaaaa atggagagag cccaataaaa gctaatggta
    28201 aaattctcgt tatagatggt ggtttttcta gagcttacca aaaaactact ggtatagctg
    28261 gatacacttt gatatacaat tctagaactt tgcaattagt atcgcatgaa ccatttaatt
    28321 ctgctgaaga agccattgca aatgaaagtg atatcttatc aacaactgtt gtagtggaac
    28381 ataaggcaaa gagaaaaatg gtgagagata ctgatgaagg agtaaaaata caagaggaaa
    28441 tagaagattt aaaactactt ttaatggctt ataagaaagg gctaataaaa gaaatgtaga
    28501 tactatagag gcacttcata attgaagtgt ctttttgtta atacttatta aatatattat
    28561 atagattaat aatagtaatt tataacctta tacttactaa gtgaatccaa gataaatggg
    28621 ttcacttttt ttaatctaaa acaaagtata aatttctaat tatcttttta tattttctta
    28681 aatgctcgta aagccttatt ctatgtgctt tcgagtattt ttactgtagg aagatacttc
    28741 acgtttcttt gcatatttcc tcatgtctta gctgtcagaa gtggtaaata agtagtaaat
    28801 tcatttgtac tactaagcaa caagacgctc ctgttgcttc tctttattca agcgtttcat
    28861 ttctgccatt gcagaatcga atgttgcatg tgcgtaatag ttcagcgtca tggctatatt
    28921 agcatgtccc ataatgtact gtaatgcctt tggattcatt cctgcatttg catagttggt
    28981 acagaatgta tgtcgcaaac tatgtggagt gatgtgtggc aatttatcct cgttatactt
    29041 attgtatttc ttaacaagac ctttcatcat gccgttgtaa tcacttgcca cttttggata
    29101 gttctttcta ttaagaaaga ggaaatcact atatccatca atctcaacac gcttatcatt
    29161 ctttcgattc gctaacactc gcttaaatgc ttgataggct tcttcaacca taggaacttg
    29221 acgttcgcca cttttggtct ttggtgtttc aatgtagtac ccaatttcag tatctctcaa
    29281 tagctgatgg tctatattga caagacgatt ctcaaaatct aaatctggaa gtgtcaaacc
    29341 accaaactct gaaatacgaa gacctgtttt taagagtatc agaatttcat cataattttt
    29401 gctgtaggtt ttatcagctt ttgcaaaggc taacagtttt tcttcctgtt cttctgttag
    29461 tacggtctta gggacagtat catcatcaag aactgctttc agttgaaagt caaatggatt
    29521 cttccgaaca caatcatctt gtatagcaat atagaatgaa gcctttaaag aacgtttgta
    29581 gttattgatg gtttgataag cataaccatt ttcactcatt ctaatagccc attctttagc
    29641 gtctgatggc ttaatactgt caatacttct tacacctaac ttgtctttct tcaaaatatc
    29701 cataagatat ttgcgtccag tttcagtgtt ttttctaacc tttggtcttt gagcgttctg
    29761 ttttgcgtaa agctggcaga gtgtcatttt ctttcctaca acatcaatac catcatgaat
    29821 gtctttctgt aactctgcga ttttctctct aagtgagata caatcacgct ttcctgctgg
    29881 tactcggtct gtagccacaa gtttccacga gtaaacaaat tgcggttctc caaatgaatc
    29941 tatatatttg tataagtatc ttccgtcttt tcgttggctc tctccagtct ttaagattcg
    30001 acctttattg tcacgtcttt tttctgacat ggcatttgct cctttccttt atggaaagag
    30061 ccttgatacg acttaatact attttatcat atacaagacc ctttggcgac gctagattgc
    30121 gtccaatgta tctataattt tttcaaattg ttttcgttta atctgaatac gattgccatt
    30181 cataatcagc caatttgcat ttttattttc ctctgccaag cgtcgtagct tgttttcgcc
    30241 aatacgaaaa tattttgacg cttcttcaat ggttagggta taacgttccc aaataggaat
    30301 gtcagtctgc ttcataaaat cctcctttcc aaatcactta tttggatttc ataaaagttg
    30361 ttttaccagc aatcgaacag ctttagcaaa gctcacggga gttccacccc tgcatggttc
    30421 tcatgtagcc atactcattg cctgcgacgg ttttatcacg ctcggactat tgactgtatg
    30481 ggagtatcat tatcacgata agaatgtcgt tgcaggcaat cctgctaaag attgcttctc
    30541 ggatcactaa catgaatcgc tcgctatctt tataagatag gtcatggcgg ttagttccgt
    30601 tggctctttt cttatcgaaa cgtattcgat tacttttatt cagttttcaa agaacaatgg
    30661 ctcgttagcc tatcaaaaca cattgaaagc tcaatatgct ttggtggaat aacaaacctc
    30721 cctgttcggg aagcgtggaa tggtttagca cgcttccacg aaaggagaga ggatattact
    30781 taatttcaaa tgacaaaatc tttgtaatca gtctggtttc cattcttcca cgtaagactt
    30841 catcaacgac catactttga ttgccatatt catctttcat aagtcgtagg gaacgcttcg
    30901 ttatgtaccc tctgtaatga tgtagaatct ggttaatcgc ttcggtatcg ccatctgttg
    30961 cctttacaat gagaggaaag ggaatcatag gatattgtgt tttcattctt caaattcctc
    31021 cataaacttt ttaattaagg ctagtccact ggttctatgc cgatagacag tagaacggtt
    31081 caatttcaac aggtctgcaa tttctgaatc gctcatgtcc ataaagtaaa acagcagtag
    31141 aatttcacgt ttcttgtctg gcaactcacg taatgcttca ctcaacaaat cattttcaac
    31201 gcctactgat aacccattga gtgtaaaaat ctgaaagtca gttgaatagt tatctgttgt
    31261 cgcaaactgg ctaacaagat aatcgccaac atccgaaaag gacacctcac gctttgcaat
    31321 ccttgaaaga taaagcatat aattctttcg ctcgtcttcc atagcacgtt tacagatata
    31381 gtcaaactga ttttctattg tggtctgaaa agaagatggt ttcatgtttc tcaccccctt
    31441 tctgtctagg aaaggaagtg agccttgctc gtttatctcc tttcactctt agtcccaatg
    31501 tgaaaggggg atttgttgca ttactgataa ataaactttg taaaaaagtt ctgaatagcc
    31561 aaaaaagcat ataaacagat ttatttctct gtttacatgc ttctgttatt ctatctatat
    31621 gatttataaa accacattgg tggacgtact tatctattgc agatagacga ctttttttga
    31681 caagaaccca atgtaaggaa atttattgta tatgatgtac ttcatggcga cgttgacctc
    31741 caacaaaccg ccatttggaa gtaatataca atattttaac agcgtaaata gcactaccat
    31801 ataacggttt tttttattgg cgtttagtag tgctttttat taaatataaa cctataaacc
    31861 atataacacg tttttctata cctgttttta attcagtagg aacaataaaa tgtatagagg
    31921 tggtctacta tgcgtaaaaa agaagataaa tatgatttta gagcctttgg tttagccatt
    31981 aaagaagctc gattgaaacg aggtttaact cgtgaacaag tgggagcatt gattgaaatt
    32041 gacccacggt acttaactaa tattgaaaat aaagggcaac accccagcat acaagttctt
    32101 tatgaccttg tatcgttact tcatgtttcc gttgatgaat ttttcttacc tgctaataac
    32161 ttggtaaaaa gcacccgacg attacagata gagaaataca tggatagctt tacagacaaa
    32221 gaactatcct taatggaatc tttagccagc ggtatcaacg aagcaagaaa catcgaagac
    32281 taattaaaag aatccataca taacggaaag agccgataaa atgagattgt attaatctca
    32341 ttttatcggc tctgcgtctt tgcgtctggc tctgtaatca cagttacttt gaactgcttt
    32401 atttcaatta aattttcttg tctgcatttc ggacaataga gggggaattt ttttaattca
    32461 gtatcttccc ttatctttaa tcgtgtttta tttccacata caggacacaa tatccacttg
    32521 tagtttataa taactatctc ctcctttaca ctttaattca aatctttatt aaaaaatatt
    32581 tcatcttatt taacaagaaa ccatatttat ataacaacat aaaatacact aagttatttt
    32641 attgaacata tatcgtactt tatctatccg actatttgga cgacggggct ggcaaacagg
    32701 ttcaccggta gtaacatggt acccttttaa ctctgttaaa caaacactac gtccatttgt
    32761 aaagaaagtt aaatcactac gatattcttg aatacaccga gcagggattt ctccactaag
    32821 aatgacctca ttatttttca attgagtgtc tacgatgttc gcacaatatt taggagcatc
    32881 gttgtatgct cgtgaaagat attcctgtgg cgcataaatt ttaaaactaa gatatggctc
    32941 taacaattct gttccagctt tttttaagac ttgttccaat acaataggag caagcatccg
    33001 aaaatctgct ggggtactaa cagggctata gtataagcca tacttaaaac agattttaca
    33061 gtccgtcaca ttccaaccat acaatccttg ttcacaacca tagcgtatcc cttccataac
    33121 tgcattttga aatgattgat ttaagtatcc aagagaaacc gagctctcat actgcattcc
    33181 acttcccaac ggaagcggtg atacagataa accaatggaa gcccagaaag gatttggcgg
    33241 cacttcgatg tgaatggtat attctgcatt ttttaacggt ctctccatat aaatgactgt
    33301 aggctctttt agttctatct ccacatgata cttttcttgc aacagtgcac taatcacttc
    33361 catttgtact ttccctaaga aagaaagtat aatttcatgt gtcgtagaat ccacgtaata
    33421 tcgtagaagc ggatcactat ctgagatttc caaaagggca tcaagcaaca tttctctctg
    33481 ttcaggttta ctcggttcaa cagttgtttg tagtagaggg tgcggatttt caatcttttt
    33541 tctctgtggc aatagttttg tatctccaag aacactattt aacttcaaaa actcattttg
    33601 caaaataaca atttctccag aataagctct atcaatctta cataattcac catttattga
    33661 agtatacatt tctgtaactt ttattttttc tttttctgat actctaaccg aatctcgtaa
    33721 atgtagtact ccactataaa ggcgtatata tgcaagacgt tgtctttttt ttgtatattc
    33781 aattttgaaa acatttccgc aaagttcaga cggacctcga tgtgttgatg aataaaattt
    33841 attagtaata acttctataa ggttatcaat ccctatatta ctttttgcac ttccatgata
    33901 aagagggaac agagaacaat tctgaaatct tatgctttcc tcttgttcga gttccaatgc
    33961 ttctaatgat ttaccggaca tatatttctc taaaaggtca tcgtttccct ctattaccgt
    34021 atcccattgt tcagattcgg taaagttcgt cacacacaca ttaggataca gttctacctt
    34081 ctgtttgatt acaatttcgg cagaaagttt ctctttaata tcctgataaa ccgttgataa
    34141 atcaattcca ttttggtcaa tcttattgat aaaaaagatt gtgggaatcc ccattttcct
    34201 aagtgcatga aataatatac gagtttgtgc ttgtacgcca tcttttgcag aaatcagtag
    34261 aattgcccca tctaaaactg ataatgaacg atatacttct gctaagaaat ccatatgtcc
    34321 tggcgtgtct atgatgttca ccttcgtatt ttcccactga aaagaggtta ttcctgtctg
    34381 aattgtaatt cctctctgac gttctaaaag cgtattatcc gtcctcgttg tacctttgtc
    34441 cacgcttcct aattctgtaa tcgctccact gttatataat aagctttctg ttaaggtagt
    34501 ttttcctgca tcaacatgag ctaaaactcc aatattaata attttcatgt gattttcctc
    34561 cattcaaaag cccaaaaggg cataaaaatc ccagtgataa atactcttat cactgggatt
    34621 tttatgcata accataggca tacaaagcat acagatattc tccggatact ttagaatcac
    34681 atgataaagg tattcttaaa ctgggtacaa aaaactaagc cctcctaaaa aaggacatcc
    34741 aattatttgt tcccgctatc aaattgacag tttatttaat aataccttgc cgcatattta
    34801 ttaactcctt ttaaatagat acttaaataa tagcacgtaa gagcatattt gtaaaggaat
    34861 ctccaatttt ttatcaaaga gagtacgtga ttacaaaata gctgtaataa tgtaccaata
    34921 tttgttattc tataatcttc caattactcc cgttcttttc aagtaccaaa tcaaattgag
    34981 atacctgcgt tgctttggtc tgctggtcga tatactccac tgtcagcgat accgtgactt
    35041 gattatcctt acgattgtga ataggattta ccagttcttg aaagatgtac tcttttccga
    35101 ttggttttaa tatcccgtca ttcacatagt aggaaagttc actggctgtc gctgtaggat
    35161 agagcttgaa gaacgtcgtt aaaaactcat tgatttcatt ggttgtaatg gaatcaaccg
    35221 tcccctcact ttcaatggct tttggtttat aacttgattt cttaggtatg ttggtaatgg
    35281 tcggattctt aaccagtacc atatttccag aaccatctac atagacactc actatataag
    35341 cagagtggac ggtctttgta ttttctccct ctgtaatgag ctggtctaca ctgtaggtta
    35401 cattaaactc attgtcgcca gttggctcta ccgtccatat ctgaaatcct cttacagaag
    35461 acgatacagg aatatctttg cgtactgtat caacattgag agcttgaagt tcatctgtca
    35521 gatagccttt tagactttcc attcgattat caatggactt atcggattgc tcccatgaat
    35581 agtagacttt cgcaaagttc tctacaaaat tttctacatg atgagtatca acgtattcct
    35641 tttctatgat agttgtttcg tgaatagtat gagtatctat agctgtaaag tgcttgaata
    35701 tcgcaaagct gaaactaagc cctaaaagta cccacaaggc aatcacaacc tttttatgag
    35761 gattgacctt atagacacga ggtttctttt cctttggtat ctgtttttct ttattctgat
    35821 tttttctaaa tttcatcatt aaatcttcct ttctcattgt ttgattcgtc ctgctcccac
    35881 taaatgctgt tgccagtagg ggcttgttaa gtcggcataa ccgattgggt cgcctgcatg
    35941 aaacatacgg ttattgccaa ggtatatccc aacatgagta atataagagc cagcgttata
    36001 ggtagaatga aagaaaacca aatcgccagc ttgtgcttcc gatagtggga tatgctgggt
    36061 cacatcatat tgctgttgtg cggttcgtgg taagttaatt ccagcttttc catacgtcca
    36121 ttgtgtcagt ccgctacaat caaaagaagt agtcggggaa gctccaccgt aaacgtatcg
    36181 ccagccctca tatttcagtg cttcgtccat gatggcttgt accgtatcat catcaaactc
    36241 tgttgtgaca agatactgcg ttaccagttg cacataaaac atattgccat agttgtatcg
    36301 ccagccccca ttgataggta tggctatggg attggggtaa gacacttttt cgccacctga
    36361 atactctttt gagaaacttt gagccagttc aaaggtatat ttatttccac gattagccac
    36421 ataccctaag aaaccaccac cataattgta ggactggata accgattcta aatctacact
    36481 gagcctttcg ctactggcta ataattcact gaaatacttc acaccttgct taatggattc
    36541 ttctgtactc aatgaattag gtggaagacc gagggattcc gaggactgca taacatcttc
    36601 cgcagtaccg cccgattcca cctgtataat cgcaagaagt atgttgacat attcttcaac
    36661 gccatattct ttggcatatt tttctaccat aggcttatga gccagcactt ctgcggaaac
    36721 attcacacct ccataatgaa tattggaaat tccgctgtcc tgttcatctg aaaataaaat
    36781 ggcaacaaac agaagcagtg agaagaccat caagaataat ccagaaccac caatcactaa
    36841 agttttcaac ttcatggttt cttaccgact ttcttaatgg tggcggtttt gattggtggt
    36901 ctacttcttg tattttgtag tggtactctt tgaacagtag acggacgttc ttttgtgatt
    36961 ggacgttgtg aagttctatc tgctgtagtg gttgaagttg ctggcttttg aacggttttt
    37021 tcttgaacgg tattgccttg gcgttccact tttggacttg aaaaatcgga cttaactgct
    37081 ggacgctctt gtttggcttg ttgagattcc ttatatgaag tctgaatatt agactgtttt
    37141 gaggtctgtt catcatgata ttgttcttgt cttgtagtcg gtctttcatg aacagaagaa
    37201 gcaggctgtt ttttctgttt gacctgttcc atttcagagc gacgcttcgc aatggttttt
    37261 cgcctttgtt cctgctgttc cttgcgtcca ctggctctgt ccgctttggt ttgagaaata
    37321 ctactggtta aatcacggac attctctttt actttggatt ttccttgata tactgcatat
    37381 cttgcattgg tcggcaaatc tttaacctgt tctttcaaac cactagcagt gtctaccatt
    37441 ctgtctttgg tatcagctac tgtaccgatg gtttgaccga tacgttttcc aagtgttgat
    37501 ttttcctttc cgtctggtcg ggagtgatct gcttgtgtcc ttgcagaact ccccgaaccc
    37561 gactgtcctt ttttacctgt aacaatggca gacccagccc ctagagtagt catggaacgt
    37621 ccaagtttcc gctgtagacg gtgcatgtga gcgtgcataa gcatacgagg ttttctcatc
    37681 acacgacttc ccacactttg agaatcgtta ctctgtagag aaaacatact cattaaatcg
    37741 cccagcttga agtagattcc tgcaaaggtc acaatctgta gaaaagcaat caaaaagaac
    37801 ggataaccag ccgataaggt atagagcatg gttgaaatac taaatgctgt cgtaataatc
    37861 aatgtgattc cagctcgtgt caaaatggta ttaaagagct ttgttatggc tcgttttgac
    37921 ataccatcaa atgatggaat catgcttaaa ataaagctca caggcagaaa catagcatag
    37981 atgataaaaa gtacctgcga gaaaatcatg attcctgtta ataggaatac aaatatggaa
    38041 atcccaatat tgaagacaaa taggaagaag actgtaccta aacggttaat ggtctttgta
    38101 atggttagat tggtattgct tctgtcttca atttcttccg caacaatttt ttctctgtct
    38161 tcgccattgt tggaatctgg gctggtggag agcaggcttt ccacacggtc aataccgata
    38221 ctttcaatgt ctgaactgtt gtattgaagc agtagccacg gttgctgaac ctgtatggaa
    38281 aacaggctat ctctgattaa gtccacgctg tccttgcctt gactatcgga atggggcatg
    38341 acaatcttcg tgccaagtga taaactggca ttactgatgt ctgatgaaaa gtcattgatt
    38401 tttttaatgt agtcgggagc gtaggcaata aaggaagccg ataggataaa caccagcaca
    38461 aaattcataa tggcatgaat tgcctttgtg gtttctctct ttatcagtcc cgtataggca
    38521 acataaaccc caagaaccaa aatcaagagt aagaggaatc caacatagaa accctctgtt
    38581 gaaaatccgt ttgcactcac accagctaag gtctgcatat tcttaccaat ggaatctgct
    38641 gtagcggaaa tgaagtctaa ggaataggct tcctgtacta agtaacctgt cgcattggaa
    38701 acatacaaac tgattgtcca aataaaattg gtaatggcat atagtccata catgacctgt
    38761 tttccaatcc cgtccgacca gttccacgga agccagcccc agctattatc cacataaaaa
    38821 tccagttgat agttttcaag tgggtatcgg ctgtattcat ttgccacatt gaccgtatca
    38881 tctaccaagc ccgcagcttg aaccaccgtt cccagcatgg ctaaaagaaa aatggcaatc
    38941 acaagtgtga aagccactgt cattgccact ttacctagac gtttcagcgt ccagtttgat
    39001 tttattctgt ttactattga tggtttcaca tttacacctc ttttcgcaca ggtggtctgg
    39061 tatcaaaggc atggagcagt tcttcaaata cagggtggaa ctgtatcaca ccgacacgac
    39121 catataaatc actgataagg cattgcccgt tttccaaatc acgcaatcgc ttctgattgt
    39181 tttcgtcctc tgggtctaca ccaaaaaagg ctaaggtctt tttaatctcg ttaaggtcag
    39241 tggaacgaaa tgcaaatttt aagccgaggt tatttttcag tttttcatct aagaggtcgt
    39301 ctgtattttg ggtcacgaaa tataccccag cgttcatagc acgaccagcc cgaaccagct
    39361 tcatagatag tgtttttcct tgtgctacct gtaaaaagct ccatgcttcg tctaaatcta
    39421 caatcttgaa aatgcttcgg tctgtatgga taaagtctaa agcaaaggta ctaatgacaa
    39481 tcagcatagc aacggataaa agctccatag tggtatattc ctcaaaggaa gtttccttgt
    39541 cgggaagtac caagtccgca acctgtataa tgttcagttg tttttctaag ctgatagact
    39601 gctccacata accattactg aataataaat gtgcaaagtc atagtctgta aaactttcga
    39661 tatggtcggc tatactggta cttagtggcg tattctcaac ccgtaattcc tcaatcactt
    39721 tcatcaaccc tcgtacttca ctattggtta ctgcacgaat ggcttttcta aggattggga
    39781 agcgttcccc atcacgagag gaaatccccg taaggaatgt cagaatatca atagccagtg
    39841 attcagaatc tttgggattt ttcataatca cataagggtc aagtaagcct ttgtttttct
    39901 catcagaagt cagagtgacg atattgattt catgggaaat ctctggcaag gtttctttcc
    39961 atctgccacg ttctgctttt gggtctacaa tcactgcttg tgccccataa agcaccgcat
    40021 aatagacgat aaggttattc gcaaaggatt taccaccacc cagcgaacca acaaaagccg
    40081 acgctaacgc attggttact gaacccttaa ccccttgact ggcaagagca ggtttcagat
    40141 agacattgcg tccagtatct aagctgtagc caacataaat cccctcattt tcccccagca
    40201 tttgagtagc accaaaacct aaaccagcga ggaaatcaga ggtcacgtat tgaatataat
    40261 cattcatata acgcttgctg gcaggtaaaa attcttcatg taagccgagc atatccccaa
    40321 atggtcgtac cagttttacg cttaaatcgt cataaaaatc tttcacttca ttacaacgac
    40381 gtttgagttc gtcaagatca tttgctgata cccttaccac ataagacagc ttgtacatag
    40441 attccttgct ttggtctaaa ttggtttcca gctcattcac actttccaga gcttccgcca
    40501 cattggagct ggtttcatta tcactttgcc aagcgtggtt atccaagtct ttcagttctt
    40561 tctttttatt gcggacagta gatagggctt tacgattcgc tacaatttcc acattcattg
    40621 acgtatcaat cgggaatgta aattgctgtt gctggtagta gaagatttca gaggacggga
    40681 agtccagttc tccgacaatg ctgttaatgg taaagtaagc tacatagacg gtttcatctt
    40741 cctgctggat tttcaaatat cgctgttttt cttccaccaa acagcgagta ggcttaatca
    40801 agtcatagta tttaatcagc gtttcattat ccagcttttt ctttgataga tggtactcat
    40861 actcttcata ggcagtgcct gtctgtccgt aaaggtgttc aatcagatag ccgaagtcgt
    40921 ccttatctaa cctgcggatt ttgaaacgac gagagatttt attttctaag agcttttcca
    40981 tcttctgaaa acgcaggatt tcatcattac tcatactaac aaaatcgccc atcagcttat
    41041 ggttcacatc atagacaaaa tcagacaaag cattttttgc ttcaacggta agacttttca
    41101 tagaaaactc ctgatcgttg agaagcaact taaagccgat aaagaaacgg tagttcactt
    41161 gattttcgcc aatcatggat attaaagcgt ctgtctgttg gtcgattttg tcataggcaa
    41221 ccgctttgag cttgccagtg acttcatttt tggaacgctc ttgtgcagaa cgtatgctgg
    41281 attctgtact gatttgtaaa gcatgaattt tgccatcacg attttgtgcg ataagctgtc
    41341 tgaaagaatc atgcacttgt attttctgtt ctggacttag aaatgagtaa ttgtaaggaa
    41401 caagctcata gtaagcataa cattccccgt ctttattcca gacgagattg ttttcaatgt
    41461 atttaattgg atatgccata aaattcactc ctaactgctg taatggcttc ttgtggctgg
    41521 tttctgccaa gcgttacttt ttttcctgca taggtcagct ttggtcgcag tgcataagca
    41581 atgacagact tcaaaaatcc ataaggcttt ttaccatcaa aagtttttgt agacataaac
    41641 catgtgaaag ccacaggaat cccaaagtat ttgagaaatg ctccctctat catggaaaga
    41701 gggggcaagt tgccaagtat catcactgca aagagtgaca cgacaaacca tgtcatttgc
    41761 gtaaaggtta tgggaaacgg aagtctaaaa tcattgatag aatacagtac cttttccaca
    41821 gaccagatac tggtatagct tcgtattttc ttcatgtaat caatcctttc ataaaaaata
    41881 ggggtagctg attgagccac cccgtaaaat agaaaatctg ccagtagtaa tgtaccgaca
    41941 gatttaatag acgatttcaa aaatcccatg attggttgag ataaacgttc ctgaaaggtc
    42001 taaatcccga ccataggctt gataatcaat atagttttga agactagctg gtacttcgcc
    42061 taaagcaccc gtttcttcaa tgtagtagcg tgccacgtca tacatatcat cacaatcgga
    42121 atgaatgata atatcctctt gatgttcgct tagttcttca atgcttgaaa aatgagtgag
    42181 cagagcagat agctccgatt gtaattcttc gggtaattcc gataccattt cccatagtcg
    42241 attgagttcg ccaatggaag tgtattcgtc aaccgtaaag ggtaactcgt agtcatgaat
    42301 ggcgtattcc tcatattcat cattcaagcc gattttctct ttgacttcct caaagtcaat
    42361 gggaaaggta aaccacgcac cgaccaattc gccctcattg tatttgccta aattcgcaat
    42421 atagacttgc atatcgtcca tatattcacg tcctttcttt gtagagattc aaaaatccct
    42481 accgcacttc gtttggtgta ccattccttt gcggaacata agaaaaccac ttatattcca
    42541 caaaagaacg gttttattta agcaccaata atgcgattga atagctctag taaaatgtct
    42601 tttactccag cagcgttgaa gactaagcca accgcaataa tcgcaataat taaaaagcca
    42661 atcagtttgc taaactcacg cttgaagcca agatacaagc caatcacaac gattgctaaa
    42721 agcaccagtg attgagcgtt tgatagaaac cagttataaa ggttttgtcc aaaattcata
    42781 aaaatgttct cctctctata ttcaatgaat ttgtatttga gttatttttt tgttgttatc
    42841 acgtcctgtt cttttactga ctgttgcttc aaaatctgct tgtgtcggtc tgtcagtttc
    42901 gcatggtcga gaatgtcttt tacaacctgc gtctggttga tttcatcaag tttaatcgca
    42961 acctttaagg tcggggcaac ttgatgagat agccagttca gcgtcctttg gaaggagtaa
    43021 ggctctggtt ttgtggttag ttttaatcgt tcacgattgt tcccaataaa ccaagcccat
    43081 tcttcattca gtttccaatc agaacgaggt ttggaatcgt ctttatctac aaaacggata
    43141 taccgattga taattttaaa ggcggtatgc tctggattgt catagacgag taaatcacgg
    43201 actgcataat aggcacgctc atttttcaat cgaatctcaa aacggttttt tacttctgcg
    43261 tcttcaatgg gaatatcatt tttcttgtac tgctcgtagt ccttttcata gatacagaaa
    43321 taaacttcac tttgtaatga accgatatag agggtgtttc ccatacattc cttttcctct
    43381 ttgcgtacca gttcgccact gcgatagctt ttaaaactgc ggaagacgga gatacattct
    43441 tcctgttggc acttttcagt gagtacaggg atattcaaaa tccctgtctt atcgttaatg
    43501 gcaaggtcaa ggcgtttcat cacaccgcca gccaccaaaa cgtccataaa gaactcatac
    43561 cagcttcttt gttgtgccag aagatagctt tcaaattgtc tgcacccacg acctttcaat
    43621 tccaccagaa ctcctttgtc cagttcatgg gagcaaagga cgaatatgtc gcctaaagca
    43681 taatgctctg aataagaata gaaaccatag tcctcatgaa gaaaatagga cagtttcagt
    43741 tgtaagatgt tttcgaccac ctgctgtacg tctgttgtcg gaaagcgaat ccttacataa
    43801 tcaaacagca tttcaagggg agcgtcggga ttgaagcgtt ccagagcttc ccaaagggac
    43861 tgctgtaaat cctctgatgg cttgactttt cctgtttcaa tatcgcttag atactgcctt
    43921 gtaataccag tcgcaacagc taaacggttt tgagatagtc cataagccaa gcgtttttct
    43981 tttaaatgct gtaaccaagt ttgttcattc agtaaaaatc cctccaatca aaaaggcgta
    44041 tgtcaacttt taaagcccat ttgacatacg ctgaaatttt gtaaatccct tgtaaccaaa
    44101 ggattttcta atgttttttt gactgtttcc tgtcgatttg tacccccctg ttagatacgg
    44161 ggggttaagt gctggcgtgg ctattgccac accagccagc aagatcagtc cacacctgcg
    44221 acttccgctt cgcacgtcgc ctgcgtggac tgtctgctgt tggataactt tttaatttcc
    44281 tccaagaaat catatccttt tggtacaagg ggagtataaa actctgatat gacacttgtt
    44341 cctacatcaa catagccacg acctttgatt cgctttaaga agaaatcctt ttgtacgtca
    44401 ctgccaaaca tcatgccata gcccatttca gacatacgac ctaaagccac tctgaaatta
    44461 aactgatcac ggattccgtc gcctaaatat tttgcgtctg gacgttgaca agccagtatt
    44521 agaaagaagc cagcttgacg acctaacatg acaatctgtt tcagcttatt cataactgcg
    44581 gtgttttctt ttgttcccag catttccatg aaagcgacgt attcatcaaa gattaagaag
    44641 tgtgccggga gacctaagta agcataattt ttgccagtct tatagttctt catctgcttc
    44701 atttcctcac tacgtttcat catttcttca tagaatgttt caatgcaaga aagcaagtct
    44761 tcttttctat agtagacatt tgccatcaca gaacctaagt ccgcaaggtc agcatttttc
    44821 gggtcaagaa tatacagttt tgaatctgta tgaagcaagg cttcaatcag tgtcagtata
    44881 aagtaagttt taccgccacc tgtaccacca gcaatcaaca tatgagggag cttatcatat
    44941 tcccaccata cgtttttcat taagcgaagt ttaccatctt tagcttctac ttcatcaata
    45001 gaaatacgac tggctatggt gtcatagagc aaagtatatt ccacatagga atcctttaac
    45061 tctttatccg tcagctcaca gtacaagcca ctctctaatt tcttttccaa gtgtaagagt
    45121 tggtcttgat attttcccag cgtgatttcc acccgtatct gtatcaagcc atttttaagt
    45181 cgataataca ttttagggaa gtaggttatc ttttcctttg tacgaccagc actatcttta
    45241 aagaaaccct ctgttttgac ctgttcagat tcataccact tgttttcaag tatcatcttt
    45301 gccagttttt gacggtggta aagttgttta accgtatcat agcgaacccg tttgaataca
    45361 aacgctacca gcaagcagat aagaattgcg acactgaaac tgataattaa atagggaatg
    45421 tcaatcttat ctgcttgtga taggttaaaa tcctgccagt tgatctgctg gattgtcttc
    45481 acatgaaaca gtccgacaac cagcaggaaa acaggcagga gtgacgctat cgtaaaatga
    45541 aagactaaat ctttaccaga tgggcgaatc cttttaccac gctgtttcat gcgaaaaagt
    45601 ctcctttcta cctagcgact atttgtcttg tgtcggttct ttctttgctt gtggttgagc
    45661 tttgaatgaa ctagaatcct ttgtcagcac aatatcgtct gccttgatat accagtcaac
    45721 atctgctcct tgataggtgg cagtagcaac ggtgtccgca atgggattga taagttccac
    45781 ccgtgcgtta taatcaaact ctttcaaagg cacgctggca ggaatactta cttgaatcat
    45841 gcgtccttgt cctttggatt ttaagtcata ggtacgttcc ttgatttcat ctgaaaccga
    45901 cccgtcttca ttttggattc tcacttcacg acgtagagca gagaatttca attctccaaa
    45961 agtcgtgtct ttatctaata caatgccatt tgctaatctc atcatttttc ctctctttct
    46021 ttattctttt atcatgtcgt cagcatgtaa aaggtaattt gtaaaaccac gagtgccgat
    46081 tttgtagccc tctgcggtaa tacgtggatt gactaacttc acacgttcct caaagccgaa
    46141 atgtttttcg ccagcttcag caggaagcac caccacaata tcatctgctc tttgaacatc
    46201 agaatagaga ttatagcttc ttgataagac agttagccgt ccgttgattc ttcgctgaac
    46261 gactttatcc tcgccagcaa attctaaatt gccgaatgtt ttttccatgt tgggaatcac
    46321 aaatttaagt tccatatttt tacctatcct ttctttttta ttggctgaat gaatgtttga
    46381 tggtcttaaa gagtggggaa cgaccttttg attcttgatt ttttgttttc ataagttcac
    46441 ttcctttcaa aatcgggtaa aaaaatagac acctcatttt ttgaagtgtc tacctattaa
    46501 atattcaaat tttattggaa gtatctttat atcttcactt ttcaaggata aatcgtcgta
    46561 tcaaagctca ttcataagta gtaaattagt agtaaattga gtggttttga ccttgataaa
    46621 gtgtgataag tccagttttt atgcggataa ctagattttt atgctatttt taatctaaaa
    46681 cattataaaa aaatatagat gttttggtta tattccttgt cctttataaa tagaattata
    46741 taaacaaagg aggaggacaa gaaatgaata aactttctga tgaaataata aactctcttt
    46801 ctacaactat aagagaaaag gtagaaaaaa tctcaaacaa taatctaaac atagaagaaa
    46861 ttaggttacg ctcacaaaaa ccattgatac taaatgcaaa ctctaaagat tatttttaca
    46921 atcaaaaaac tatggcactc gatttaaatc aacaaaactc atatgtagta acaagagaag
    46981 atgtagagca aacttttcaa ataatatgta aatattcaat acattcattt atggatgaca
    47041 taaaaaaagg atttataact ttgagaggag gtcatagagt aggattagtt gggaaagtaa
    47101 tagtagaaga tggacaagtc aaaaatataa agcatatatc atctttaaat ataagggtat
    47161 caagagaaat tattggatgc tctgataaaa ttttaagtca tatcataaag ggaaaaaatc
    47221 agattaataa tactttgata atatcaccac ctcaatgtgg aaaaacaact ttaataagag
    47281 atatagtaag aaatttaagt aatggtaatg aagattatgg atttaaaggt ttaaaagtag
    47341 ctttagtaga tgagcgtaat gagatagcag gagcttatct aggtgttcca caaatggatg
    47401 ttggaataag gactgatata atagaaactt gcccaaaaga cttgggtatt acgatgcttt
    47461 taaggtctat gtcaccaaat gttattgtga cagatgaaat tggaagtgaa aaagaaataa
    47521 aggcattata cacagcatta aatggaggta taggattaat aactactgtg catggagatt
    47581 caatagatga tatacaaaat agaaaagaat taaatagact tctggataaa gagttattta
    47641 aaaaagttat aattctttcc gcaaaaagag gagcaggaac gattgaaaaa atttatgatt
    47701 tagaagaaaa gagatggtat tttgcaaatt aaaataatta ttatagctct cttgatagga
    47761 agtagttatt tgataggtga acaaatatac aaaacttata ctcgtagaca taaacaattg
    47821 aacgatttaa taagagtttt agaaattttg aggatggatt tatctttcgg actttataca
    47881 ttggaagaaa tctttaatag aataggtaga aataaagagt tttgtttttg gaaatttttc
    47941 tatcaaatat cagaaggact tcacaatgaa caaagtaaaa ctttggaaat aataatatca
    48001 gagaatattg atgtcttaag caaagaaact tatctaggca ataaagaaat tgaggaactg
    48061 aaaaatctaa tacttacttt agggaaaagc gatatagagt cacaacaaag aatgatagat
    48121 ttatctatag aaaatttgaa aaaacaaacc tatgagacaa aggaagatat aaacaaaaaa
    48181 ggcgtattgt ataaaaaatt agtgacattt ataggtatag gtatatgtat tatattgatt
    48241 taatatgggg aggttcatta tggaaatatc attgatatta aaagttgcag gtgtcggtat
    48301 tctaatatca gtattgaata tgatattgga aaaaacagat agaaaagatt gggcaggtct
    48361 tactacatta gcaggtgtaa taatcgtatt gggtatggta ataacagaaa taagtgattt
    48421 gtttaatact gtaagaacaa tgtttcaact ttactaaaga ggagggagta atttggaaat
    48481 aatgcaatta ataggaattg caatcatttc aactacactc tgtttagtaa ttaaaaaaga
    48541 tagaccagag atagcaaatt tcatagcaat aataactgga gtaataatac tattgtcagt
    48601 tatgtttaag ctaaatttta tagtagatag tgtacaagat ttggctaaca aggctaacat
    48661 cccaactatg tatatatcat taataataaa attaattggg atagcatacc taatggaatt
    48721 tgcgatacaa ctttgtaaag attgtgggga aggaaacatt gcttcaaaac tagaatttgg
    48781 aggtaaaata atagttatgt caatgtcttt ccctatactt ttgtccattg tagaaatggt
    48841 ggtgaatata attccataga tgtgtatctt ggaatgacta taagtatatt atagagcttt
    48901 cgttgtattg aggtttatag agtgaagaga ggcatggttg cttatgaaaa aaagattttt
    48961 accaatgatt ataggttttt tatttacttt cttttttgta aatatctttg ctattatgat
    49021 atttgctaat gaagtgccaa gtagtgaaaa taaagaatat ggtgaaacaa aaaatagcat
    49081 agataaatat atagatggtc aattagacaa gctggatata aatgaaattc aagactatat
    49141 aaataaagaa atagttatta atgatgttaa tttaaaatca tttgtaaaag atttaattag
    49201 tggagaaaaa aatatcttag atttattcaa taaagatggt ttgaagatat tgatgtttga
    49261 tgaatttaaa gcaagtttaa aggtagtagc agtgattttg gtattggctc tgttatcatc
    49321 cattttaaag agtctggaaa attctttttc atcaggtgca gtaagtcaaa tagcaacata
    49381 tataatattt atcacaatgg tatcacttac acttgttggt tttaaagatg tattgcagat
    49441 ttgctatgat gcaatagacc atactgtagg tcttatgcaa gttataatgc caattttaat
    49501 aacattttta cttttaattg gattcccaat aacgtcgact actttgaatc ctattttcat
    49561 aggaggagta acattcataa atgtattttt taaaaatttt ctatttgtat ctattactgt
    49621 tgcatttgga attttaataa taaataattt atcaaaaaat ataaggctaa agagattttt
    49681 ctcctttgta aaacaaataa attatgtttc tattggagca atgtttacta tttacttagg
    49741 tcttgtatct atacaaggat tgtatgtaac cagttttgat aaattcagtg taaaaactgc
    49801 taaatttgct ataggtaatt ttattcctgt tgtcggggga tttgtatccg attctgtaga
    49861 tattttatta tcttcatctc agcttataaa aaatatattt ggaggaatag ggcttatatt
    49921 acttgttgga atttgtttac ttccagtgat aaaaatttta tcagtaatag tagtatacaa
    49981 attagcagca ataattgtag aacctgttgg agaggatgga atatctaatt ttttaaatga
    50041 agtggcaaat ttaatgataa taatgctagc atcagtaatt gctataacag taatgttctt
    50101 tgtaactgta gctatcttga cctcgataag tgtcgtaagt caaggataat ttatatatca
    50161 attaaaaata attcatttaa tctaaagttt ttagagagga ggatgctatg ttagagggca
    50221 taaaggcgtg gatagttagt gttttgatag gagcatttat agtaaatata gtagatatga
    50281 ttttaccaag ttctaagata aaaccttatg taaacctagt tttaaatttt atgtttgtat
    50341 ttattgtaat aactccagtg gtaggatttt tttcaaaaga tatgagttta gaagatagaa
    50401 tattgaaatc tatgggaagc tacaacaaac agtatgtaga tagtacaaat gctttagcaa
    50461 aagagactgg aaataatagt ctatctaagg gatatgagga tgggttaaaa gaagtactta
    50521 aattaaaact tgatgaatat ggttatgatt tagaagatat agagcttaat ggagcaaata
    50581 taaataatat aaaaataaaa gaaaagaaca ataatgcaaa aagtaatagc agtgccaatg
    50641 aagaaaataa aaataatagt actaaaagag aggaagaaaa cataaactct aatgacaaag
    50701 aaaattctaa acaagtcttc aaaaaaggta cagagtatgg gttgagccta aatgaagaaa
    50761 aattaaaaaa tgacttaata aaggtgctgg atgtttctat agaagatata caaattgata
    50821 aataggagtt gggagaatgt tgaaaaactt aaatgacaaa gataagagaa aggtatattc
    50881 actaataact atagcaggaa tatgtgtggt ttcattagta ttattatctt gttttcctag
    50941 tagcaaaaat gataaacaag ttggaaaaac agagaccaat aaaaatgctg aaaagcaagt
    51001 cactaaagaa caagaaaaag atgatttaga atcaaaatta acagcaatac tatctaaaat
    51061 tgatggagca ggagatgttg atgtaatggt tacatttgaa tcaagtgaag aaatacaacc
    51121 tgcttttaat tcaaataata caacagaaac aacagaggaa aaagatgcac aaggtggaga
    51181 aagaacagtt acgacttcta gtgaaaataa aacaatgata acttcaaatt caagtgaccc
    51241 tgtagttata aaaactacag agccaaagat aaagggagtg atagtggtag caagtggagc
    51301 aagtgaccca agcgttaaag agacacttta tagtgcagta caaacatcat tacaagtagc
    51361 tggtcatcaa gtagaaatat attctaaata aatacatata accgggggga taaatgaatg
    51421 aagtttaatt ataagggaag aggatttgta ataataactt taactgcaat gttagtggta
    51481 gtaggaacag tcaattatca attaagtaag aaatctttat tagaaacatc aaaagagttt
    51541 aaggcatatg aagaagcgca gttacaaaaa aatactgatg atagtgatag tagtaagaca
    51601 gaggattcta ataatgtaga taaacaagat ggtaaggaaa gtgctgatat tgatatagtt
    51661 gatagtaaaa ctagtaaagt aaaagaaaag gcaactgaga caagtaagga aataaaagct
    51721 caattatcat cagagaaaaa tatgaaaaag gcatcttata ttttagatat gaaaatgaat
    51781 agagaaaaac aaagaaatga gttggttcaa gatttaaatg aaatgataaa taatccatct
    51841 acaacagaag aatctagaaa agaagcttct aatatgaaat taaatatagt taaaatacaa
    51901 gaaaaagagc ttcaaataga aaatcttttg agtacaaaag gatatgaaga agcattagtt
    51961 tatataagtg ataacaaagt taatgtagtt gtaaacgaag ctaaattaga aaagaaagat
    52021 gcagcaaaaa tatttgattt agttgcagaa caagccaatg taaagtatga aaatattaaa
    52081 cttacaaata ataataataa gtaaagtata aacctcagaa ttaactgagg ttattttctt
    52141 gcaaagtaga cttgtatttg gtattattat acttataaaa atagttcctt aaaatcagga
    52201 ggtaagtttt aatatggaag ataataaatt tggacaagta aaaatatcta atgatgtaat
    52261 agctacaata gctggtttag cagcattaga agttgaaggc atagaaacta cagcaacatt
    52321 aacagataag ttgttaaaaa ataatggagt aaaaatacaa atcgaagaag aggatgttaa
    52381 tctagatgta atggttacga taaagtatgg aatgtcaata ccagatacag cttttaaagt
    52441 tcaagaaaat gtaaaaaata ctgttgagac aatgacagga ctgaaggttt ctcaagtaaa
    52501 tatacacatt caaggaatca gctttaagaa ggataaagtt gataaagaag aagcaaaagc
    52561 aactaagaaa aattaatatt agccctctga aaagtcagag ggttttttgc tgataattga
    52621 ttttatatgg ataattaaac ttaaaggaga attttaattg atgaaaaaag atagggcaca
    52681 aaagagtaca acaagagaat atataatgaa acttatatac caaataaata ttaataaaga
    52741 agattttgaa actttagaag atagggtaga taatttttta aaagataatt cagaacatat
    52801 tataaataga tataaggagc ttgcacttca gtattcaaaa aatacaaatt taaagttaga
    52861 agatactgaa attgaagatg ttatagacaa aaaatatata aatacagtat gcaaagcact
    52921 aaaagaaaat catgacaaga tagatgagtt aataaataag catgctaaaa actggacagt
    52981 agatagaatg ccaaaagtag atgtttctat acttagatta tctgtgtgtg aaatacttta
    53041 tttagacact cctaataaag tatcaataaa tgaagcagta gaacttgcaa aaatatattg
    53101 tgatgataaa tctcctaaat ttataaatgg tatattggga agtgttgttg atgaaattgg
    53161 gaaataatat aataattgga attgatacta gctgctatac tacttctata gcagctatct
    53221 ctttagataa gaaagttata ttcaatgaaa aaataatgtt agaagtaatg gataattcaa
    53281 aaggtttaag gcaaagtgaa gctgtattcc aacatgtcaa taatttaggg attcttagtg
    53341 ataggataaa atcttttaaa gataggtttc atgtagaagg ggtttgctca tcaaaaaaac
    53401 ctagacctgt tgaaaattca tatatgcctg tattcaatgt aggtcataat tttgggagat
    53461 tattgtctag tatatatggt tgtagatttt atgagactac tcatcaagag aatcacattg
    53521 aagcaagttt attaaatagt aaattaaaaa ataataatag atttatttct gttcatatgt
    53581 ctggtggtac tacagagata cttttaacaa gcaaacaaga tagtcatcac aatgtatgcg
    53641 atattgattt aagtaaattt gccaaaacaa gtattaaaaa agatgataaa agtaagttat
    53701 acaataattt tggatataat atagatataa ttggaggaag taaagatatt agctttggtc
    53761 aacttataga tagggtagga gttaagcttg gatataaatt tccttcagga aagtatttag
    53821 atgaaaatgc tttaaattgt aatcttaaaa tagaaagtgg tctgaaaacc tctgtaagag
    53881 atggatatat gaatttatct gggttagaaa atcaggtaaa taagattata aaggataatg
    53941 gagataatgc taatcaaaaa gaatatatat ctaaattagt attagattca gtagttagaa
    54001 atatgtttaa atctttagta tatttatgtg aaacttataa tgtaagtgaa gtagtatttg
    54061 ctggtggagt ttctgcaagt aagtatattt ttagagaatt aaccatgaaa ttaggaaaaa
    54121 agcatataga agcttatttt acagaaccac aatattcaac tgataatgct gttgggtgtg
    54181 ctataatagg attgaataac tttttaggag aaagagtatg aaactaagag ctttagatat
    54241 aagtgaagca aattcatata ttaaaagaat tttaataaat gacccaatac tttctaatct
    54301 taaagttaaa ggagagattt ctaattttaa ggttcatagt agtggaaatg tatatttatc
    54361 acttaaagat gaaacatcaa agttaaactg tgtaattttt aaaagtaatt ttaatcgtaa
    54421 tctaaaacta gataatggta ttaaagttat agctaatgga tatatatcag tttatgaaag
    54481 agatggtgcg tatcaattat acattaatga aattgaaata gagggaatag ggaatttaca
    54541 catagaattt aatagattaa aggaaaaact taataaagaa ggattatttg accctaaata
    54601 caagatacct atacctaaaa tgccaaactc aattggcgtt ataacatctc caacaggtgc
    54661 tgttataaga gatataataa atgtgataag aagaagatat ccgaaagtta atataaagct
    54721 ttatccagta acagttcaag gagataaatc agctgaagaa atctgtgaag caattagatt
    54781 tttcaatcat atgaagaatg ttgatacact tatagttggt agaggtgggg gttcaataga
    54841 agaactttgg tcttttaatg atgaaatggt agcaagagag gtttttaatt ctcaaattcc
    54901 gataatatca gcagttgggc atgaaactga ttttactatt tgtgattttg tatctgatat
    54961 gagagcacca acaccgtcag cagctgctga gattgctact ccatcattgg atgatataaa
    55021 ctataagctt ggaaacataa aaagtaggtt gagtaagtcc cttacaaatc aaatagagct
    55081 agaccaatat agattagaag cagtatttaa taagataaat aactatttag attcttacac
    55141 aataaaagat aaagttatac aattggacaa aatatatgat aaaataattt ttggaataga
    55201 aaataattta agattagaag atgaaaagtt agtaaaaatt ggagcattac ttcataattt
    55261 aagtccatta gctactatgg atagaggtta tagtatagct caaaaaaatg gaaaagtaat
    55321 aaatagtata aaaggattaa aaataaagga tagtatagat atagtattaa aagatggaaa
    55381 tttagaatgt atgatagaaa aaattgaaaa taaagaggta tagtatgaat ttaacctacg
    55441 aagaagctta taaaagacta gagagcattt taaatgagtt agaatctaaa aatgctagct
    55501 tggatgaatc tttaagccta tatgaagaag gtataagcct ttataaacat tgtaataagc
    55561 ttttagatga tgctaaattg aaaataagta aatttaatca attaggaata gaagaagact
    55621 ttaaaataga ggaggaataa aattggaatt taaacaatgc ctaaaagaaa aagcaagttt
    55681 tgtagaaaaa gtacttaaag aatatatgcc taaagaagaa gggtatcaaa aaactgttat
    55741 tgaagctatg aactatagtt taagtgctgg aggtaagaga ttaagaccaa tacttacatt
    55801 agaggcatgt aaaatagttg gaggaaatga agatgaagct attccttttg ctattgcaat
    55861 tgaaatgata catacatact cactaataca tgatgatttg ccagctcttg acaatgatga
    55921 tttaagaaga ggaagaccaa caaatcataa agtatatgga gaagctatgg ggattttagc
    55981 aggagatgcc ttgttaaact atgcttttga agtaatgctt gcaggctcaa tcaataaaga
    56041 aaatccagaa aagtatctaa aagctataaa tgaaattgcc aaaggtgctg gtatatatgg
    56101 aatgataggt ggtcaagtag tagatgtaga aagcgaaaac aaacagattg aaaaagaaaa
    56161 attagattat atacatatga ataaaactgc tgcaatgatg gtaggctgta tgagagcagg
    56221 agctactata ggtggagcaa attctgaaca aatggaagaa ataactaaat atgcaaaaaa
    56281 tatcggatta tcattccaga tagtagatga tatactagac atagtaggag atgaatctaa
    56341 gttaggtaaa aaggttggaa gtgatataga aaatcataag tcaacatacc catctctttt
    56401 aggtttagat aaatctaaag aaattgcaca taatttgatt gatgaagcaa agaaaagtat
    56461 agaaaaatta tctgatgatg tagatttttt aaatgggttg gctgaatata taatagatag
    56521 agaatactaa aatatacagc taaaatcgag gagggaaaaa tggatttttt ttcggagatt
    56581 tttaacaatg gggctttggg aataagtctg attgcttgtt tcttagcaca atttataaaa
    56641 atatttactg gtaaggaaaa aagaattgag ttatcaagaa tacttatttc aggaggcatg
    56701 cctagttccc atagctcttt tgttacaagt ttagctacag tggtaggtat tgaaaaaggg
    56761 tttaattcta ctgattttgc tataattacc gtgcttgcct taattataat gtatgatgca
    56821 gcaggtgtta gacgggctgt tggtaaacaa gctactatat taaatcaaat ggtggcagat
    56881 atacagcatg ggaagcatat agagcagaag aaattaaaag aattaatagg acatacacca
    56941 ctagaagttt ggtttggagc tttacttgga gtagtaacag ctttaatatt gatgtaaatt
    57001 gaaaatttac agaataaagg tgaggataat atgtataaat atttagataa agtaaattct
    57061 ccaaaggata taaaaaatat gagtatagaa gaaatggatt tacttgcaaa ggatataaga
    57121 aagttcttag taaaatctgt ttcaaagaca ggaggacact tagcttctaa tctaggagtg
    57181 gttgagttaa cattagcact tcataaagta tttgacagcc ctaaagataa gattgtatgg
    57241 gatgtaggac atcaatctta tgttcataaa atagttactg gaagaaaaga ttgttttgta
    57301 tctttaagac agtttaatgg actaagtggt tttccaaagg agagtgaaag tcctcatgat
    57361 atttttgaca cagggcatag cagtacatct atttctatag ctacaggtat tgcctgtgct
    57421 agggatataa aaaaagaaaa ctatagtgtt atatctgtga taggagatgg ttctataaca
    57481 gggggtatgg cgcttgaagc tttaaatcaa ttgggatata taaataccaa tatgatagta
    57541 atccttaatg ataatgagat gtccatagat aaaaatgttg gaggtatgtc taaatatctg
    57601 tcaagtatta taaggaactc aacagttgag aaaatgacag atgaagttga taaaatttta
    57661 aatgttacac aaacaggtga aattttatct aagactgcac atagatttaa agataaacta
    57721 atgtatagct tttctcctca ggactgttca ttttttgatt cattaggtat aagatattat
    57781 ggacctatag atggtcataa tactaaagac ttaatagata tattgagaaa agctaaacac
    57841 aaaaaaggac ctgttctttt gcatgtaatt acgaaaaaag gaaaagggta caggtttgca
    57901 gaagaacaac ctgataaata tcatggagta tcaaagtttg atataaagac aggtgttaca
    57961 tctgggaaag tcaagtctat gtcaactagt gttggagaaa aacttgttga tatggctaat
    58021 aataatgaaa atatagtagc aataacagct gcaatgccat ctggtacagg gctaaaccta
    58081 tttgaaagtg catatccaaa aagatattat gatgttggta tagctgaaca acatgcaaca
    58141 ggatttgcag caggtcttgc aaaaaatggt atgaaacctt attttgctgt gtactcatca
    58201 tttttgcaaa gggcatatga ccaagttatt cacgatgtat gcataacaaa aaagccagtt
    58261 acttttctta tagatagagc tggattagtt ggaaatgatg gagaaactca tcatggtatg
    58321 tttgatttaa gttacttaaa ttctattcca aatattgtag taatggcgcc aaaagacaca
    58381 agagagttgg aacttatgat ggatttgtca ttaaaattag attgtccact agcaattaga
    58441 tatccaagag gaagtagtta ttacctagat aagggagaat atggagagat tgtattgggt
    58501 aagtatgagg tattagataa tgggcaagat acagttatac tttgtattgg aagtatggta
    58561 aaacatgcct tagaggctaa agaaatatta tcaagagaag gaataaatcc gacaatagtt
    58621 aatgctagat tcttaaagcc aattgatgaa gatatgttaa aagtattatt aaaaaatcat
    58681 aaaaatgtag ttactataga ggataatatt gtaactggag gatttggaag tagaataaat
    58741 aagtttatta tagataatga atataatgta aatatactta atatagcaat accagaagaa
    58801 tttgtaaaac atggcaatgc agacgaatta tatgattttg tgggattatc gccaaagagt
    58861 attgctgata aaataaggaa attatttatt aagtaaaaat ttaatgtaga tattgtaaat
    58921 taaaacttat ataaaatata aaatttatca attagaataa atatcaagtg tgttacttca
    58981 agctacttta gttattatta aagtagcttt taacagaaag gtaattgtat gaaaaaaaga
    59041 atagatttat tgttagtaga acaagggcac tttgaaagta gagaaagagc caaaaaagca
    59101 ataatggctg gattggtttt tgttgataat caaaggtgtg ataaggctgg tacagaagta
    59161 aaagaagact gtaatataga agtaaaagga aatccaatac catatgttag tagaggcggt
    59221 ttaaaacttg aaaaagcaat gaaaaatttt gatttgacta ttgatgggaa agtatgtatg
    59281 gatataggtg catccacagg tggctttaca gattgtatgc ttaaaaatgg agcaataaaa
    59341 gtattttcta tagatgttgg ttatggtcaa cttgcatgga agttgagaca agatgatagg
    59401 gttgtgtgta tggaaagaac caatataaga aatgtgacaa tagaagatac aaagcagttt
    59461 gcagattttg catcaattga tgtatctttt atatcgttaa agttagttct tccaaaggca
    59521 aaggaattag tacgtcatga tggggaaatt gtcgctttaa ttaaacctca atttgaagca
    59581 ggaagagaaa aagtaggtaa aaaaggagta gttagagaga aatctacaca tatagaagtt
    59641 ataaaaatga tttctgattt ttcagtcgaa aatggatttg agattttagg gttagatttt
    59701 tctcctatta aaggtcctga aggcaatata gaatatttaa ttcatttgag aaatggaaat
    59761 gatggatatg aatttgatgg tgaaatatac aataacaaaa tagtagaagt tgttgaagct
    59821 tctcataatt tagaaaaata ggttaaaagg gggcttagat ttgatcctcg aattatatat
    59881 gaaaaattgc gccttagttg aagaactaag gttgaatatt gataaaaact taaatatact
    59941 tacaggtgaa actggttcag gaaaatctat aataatagat gcacttggac tttgtttagg
    60001 tgacaaatat gataggtctt ttttaagaaa aggaacagat aagggacttg ttgaggctgt
    60061 atttttttca gacaatacat atcttaaaaa aatattagaa gaaaatgata taagtataga
    60121 agatgataat ttattggtaa taaccagatt gatatactca gatggtaaga gtactgctag
    60181 agtaaatgga aggactgtta aagtgtctct tcttaaagaa attgcatcta cacttataga
    60241 tatacatggt caacatcaaa atcaagcttt atttaataaa gatactcact taaaatttct
    60301 agaccttttt ggagaaaatg aattagaaga gtttaaaatt gcttataaaa atgtgtacca
    60361 taagtatagt gaagtaaaaa aagctttaaa ttgtttgaca gaaaataaag atgagatgca
    60421 aatccaacga gaaatagatt tattaagatt tcaaataaat gaaattgaag ctgcaaatct
    60481 aaataagaat gaatatgaag atttattaaa acaaagagaa gtttatagaa atagtgaaaa
    60541 gatatataat aatctaaatt cgagttatag caagttacat aatggcgaat ataatgttat
    60601 tgacctaata ggtcttgcat ctaaagaact taatgacata tctaaatatg acagtgtttt
    60661 aagtgaatat agtgatactg tagagagaat tatgtatgag ttacaagata tatctgggga
    60721 gataagaaat tacaaagata atatagactt tgaaccttat gagctagagc agattgagct
    60781 tagaattgat gagattaata atttaagaag aaaatatgga gatagtatag acgatatatt
    60841 tgagtattat agaaaaatta aagatagatt agatgaaata ttaaatagag atgaaagagt
    60901 tgaacagtta aaaagcaaac tgatgaagat agaagaagat ttaaaaataa aagctagtaa
    60961 attgactaaa gctagaatag aagtagcaac tactttagaa aaaatacttc ttgatgaatt
    61021 aaaaagctta aatatgaaaa atgtaatgtt caaggttaat tttgaaaaag gtctatttac
    61081 tttagatgga gtagatgata ttgaatttat gatatctttt aacttaggag aagacattaa
    61141 tcctatatat aaagttgcat caggtggtga gatgtctaga tttatgttag catttaaaac
    61201 catattggct gatatagatg atattgatac attggtgttt gatgaaatag atactgggat
    61261 aagtgggata gcagcacaga ttgttggtga gaaattgagt gatatagcaa agaaaaaaca
    61321 gataatatgt ataactcatt tacctcaaat tgctgcaaat gcagacactc attactgtat
    61381 agagaaagat acttcaaata ataggacgtt taccaatgtt agtaagttaa atgaatctca
    61441 acgaaaaaat gaaattgcta gacttatagc aggtaacaat ataacagaaa aaactattga
    61501 acatgcaagt gaaatcattg aattggctaa aaagtgttag tgaaaaataa gcattttaat
    61561 aggtgtaaag aatttttata gaaatgcttt acatctattt tttatgatga aattatctta
    61621 aaatatctag ttcctatact gaagaaaagt gagtttaaaa atggaataaa atttgtataa
    61681 atttgtaact ttgaaatttc taaatgctta agtataatat tatttattaa ggtagtagtg
    61741 aggggaggca taaaatgaat aaaaaagata ctttgcactt aatttttata atcgtaattt
    61801 ttattttatt gtggtatgtt gtggtaacag taagtgagat taataaaaat attgaaagtt
    61861 gtagtaacaa gctagattat ttgtcacaag agattatgag cactaagtcg gatattagta
    61921 atactataaa tgaagaaatg tcaaaaagtt atataacaaa aagtattgac ctaaaagtaa
    61981 aaaaaatcga gaaaaaagaa tgtattattg atgtagatgt tcaattaagc aaacttggag
    62041 aaaatgggaa agtatttttt atgtacaaag aagagagtaa taaatggaat gaaactgaga
    62101 tgacaaaaca tggagaatta tcgtatgtat gtgaaataaa gataaataca ggaagtgaat
    62161 atgactataa agtagttaca agtggagcta tatctgaaag ttctgatgtg caaaaactga
    62221 ctaaatctga ttatatgcct gaacaaccta tatttagttc tgggataaga gataatagag
    62281 aatactttat tgaaatggta gaaaatagta atctttttaa tcatgagagt gaaaagtcaa
    62341 aaaataaggc ttataataat attagattag aaaaagtaga tattatcgta aatgaaggaa
    62401 aaaaagatac tgtatacaaa gctaagttag ttgaaagctt agatgatggt aaaactaata
    62461 attccaatag aaaccaagtg aattatgaag caagttttcc taaaagggat attgatgaca
    62521 taatttatat taaagctaaa cttacataca ataatggagt agaatataca aaggatataa
    62581 ctgaagatat aactatggga ttgcaagatt tagaaaatta aatacatatt taaataaata
    62641 ttcaagtgga tttgttcaat aaaatagagt ataaataaaa ctgtagtagt tttatttata
    62701 ctctattaaa aaattataat tttaattcca tatatctagc gccttcaatt ggattataag
    62761 tataaggttc tatatcataa aaaccaaact ttttatataa tccttgtgca cttttcatgc
    62821 ttggaagagt atctaacctc atgtgtgtat atccaatttt tttagcttct tcaatgattt
    62881 cttccagtag tatttttcct atttttaatc ctctaaattg atttctgaca tataatcttt
    62941 ttaattcaca tattttacct tcaagttttt ttaaagcaac acaacctgct aaattttcat
    63001 caacaaatgc caatatcaat gagcctgaag gttttttata ttttcctgga agtgttttta
    63061 attcattatt aaagtcttga aaacataagt ctatatttag agaatttgaa tattcagtaa
    63121 atagaacctt tacgttttca aggttttcta agtctttttc ttcaacaaat tgtaaagtta
    63181 tattttgagc cattttatcc gccattatat aaacatgttt atataatgac ttcacctcct
    63241 tgatatatgt atatttttct taatatcaat tagattttaa taccattact ttttcaccgc
    63301 catagtcaag ctctccatta aactcaaatc caaaatcttt ataaagctta atagccattt
    63361 tattgtcttc gaatatacta agatatagtt catcacagtt atattccttt ttaagatgat
    63421 ttacaagaaa atctagtgat gcttttccat atcctctacc ttgatatttt gaatcaatca
    63481 taaatctatc aagccaaact ctatcatctt ctaaacgacc atacatagca aatcctatta
    63541 aagaattttc atcatatatt ccaacaggta tccaagctgt tataaattta gattcagcaa
    63601 tagaaagtgc attgctttct atataatcca attgactttt atctacagac aaattaacta
    63661 aatcacgcca attatttatg tctgcaattc ttacatttaa tttcaagata ttaccgccct
    63721 tcaaactttt tgttacaata taatactatc atattatgta ttaaaaatca cttattaagg
    63781 ttttaagtga actttttgaa tgaataaatt ctcaggaaaa aggaattata aatacataat
    63841 aatacagcat aggagtagat ggatatatga acaaaataaa taaaaatttc aaaaaaattt
    63901 ggataatatt aggagctgta tcgattttta ctatggttat aattttcaat aaaaattgta
    63961 atgtaaataa agagacccaa atagaagctt ttacaattaa gaatcaagat aaaaaatttg
    64021 tatatcctat gggaaatata attggagtaa aagcaaatac agacggtgta ttggtcttag
    64081 gatatgagga agaggatgta gactatattg gaggcataca aattggtgat aatatagtaa
    64141 aaatagataa taaaaggata aaaaatagtc aagatgtatc agagattttg aataagatta
    64201 aaaaaagtaa agttgaagtg acttttgaga gaaaaaatag gtataaaaca gaagtaatag
    64261 aaactaaaaa agaaaatggc aagtataagc ttggcttatg ggtaagagat aaaatatctg
    64321 gaattggaac tatgactttt tatgacccta gtatggaaaa gttcaaagca atcggtcatg
    64381 ctataaaaga ttcggatact aatgaactat taaagataaa gcaaggatat atatataagc
    64441 cagaacaatt gaaaatagta aaggctagta atgaaaaagt aggtaaaata aaaggagatt
    64501 ttaatgacag caatttaatg ggtaatttct caaataattc agagctaggt ataagtggta
    64561 atattacaga aaaccataat aaagaattta atgtagcgaa taaagaaaag caattaatag
    64621 aagttggaag accacaagat gtaaaaatag gagatgcagt aattcttttt gaggataaaa
    64681 acaaaaacat aacaagctat gatataaaaa tagaaagtat agtgtatgat aaaggaaatt
    64741 atagagatat ggtaataaaa gtagtagatg acaagttatt ggaatacaca ggaggtatcg
    64801 tacaagggat gagtggagct ccaataatac aaaataataa aattattggt gcaataactc
    64861 atgtttttag agataatccg aaaaaaggat atggtatttt tatagatgaa atgataaaat
    64921 tgtaggtgag gcattaaaaa attttattat tttatcaatt atctaggagg aatataattt
    64981 tggagtgtcg aatatgcttt agagtagata attaggaagc aattgtgtaa aaagtttagt
    65041 tttctgtaat aagaagatgt tttttaatgg ggggattttt agtggaaaaa atcaaaatag
    65101 ttttagcaga tgacaataag gatttttgtc aggtattaaa agagtatttg tctaatgaag
    65161 atgatatcga tatattaggc atagctaagg atggaattga agcattagac ttagtaaaaa
    65221 agacacaacc ggacttatta atactagatg taataatgcc acatctagat ggattaggtg
    65281 taattgaaaa attgaatact atggatatac ctaaaatgcc aaaaataata gtactatcag
    65341 cagtgggtca agataagata actcaaagcg caataaatct aggagcagat tactacatag
    65401 taaagccatt tgattttgtt gtgtttataa acagaattag agaactagtt tctaatagag
    65461 ttacacaagt tgagccaaaa cctagaccag ttcaagagac tcaaatgact agaagtgatt
    65521 ttgttaagaa tgtaggaaat atagagacag aaatcacaaa tataatacat gaaataggag
    65581 taccagctca tataaaaggc tatttatatt taagagaagc aataaaaatg gttattgata
    65641 atgttgagct tttaggtgca gtaacgaaag agttatatcc aagtatagct aaaaaattca
    65701 atacaacacc aagtagagtt gaaagagcaa taaggcatgc gatagaagtt gcatggagta
    65761 gaggaaaagt tgacacaata aatcaactat ttggatatac ggtacacaat actaaaggaa
    65821 aaccaactaa ctcagaattt atagcaatga ttgctgataa attaagacta gagcatagta
    65881 tggttaaata aacagacata aaaagtaagg ctttttaatt aaagcattgg ctataaatgc
    65941 gcattacaag cagcgaaacg gtataaccag tagggttata ccgtttcgct gttttaataa
    66001 atataaaaaa tttccttatt atttgtttac tatatcaata taataatttt attatactat
    66061 ggatatagta tgtgtcttta caagttgtga attgacagtg gtttattttt taatataaat
    66121 attgattttg gtgcaggtaa actttgtatt tttaagcgta ttatggaata tgttaaataa
    66181 aaaaatgatg aaatatagta ttgtaaatgc caaagatgca aaacaaactt aaaacattta
    66241 ttttattgtt aagtaatgct ataatataat gtgattttaa taatgatagt ggaggtttaa
    66301 atatgagagt cgaggcccct ataaaagtag atcgaaaaac caaaaaactt gctaaaagag
    66361 ttgaaagtgg ggaaatagca gttataaatc atatagacat agatgaagtt gctgcaaact
    66421 ctttagtaga agctaaaata aaacttgtca taaatgcggc tccttctata agtgggaggt
    66481 atcccaataa aggtccagga atattaactg aaaataatat actaataatt gataatgttg
    66541 gagaagaatt atttgaagaa ttaaaagaag gagaaactat agaagttgta gatggaaaga
    66601 tatatagaaa aggaaaattt ttaggtgcag gagaagtttt agacaagtat gaggtttcat
    66661 accaaataaa ggctgcctat gagaatttag ctgtagaatt ggatagattt atagataata
    66721 ctatagatta tgctaaaaaa gaaaaaggat ttatacttgg agaagtagaa attccaaaag
    66781 tgaaaactaa ttatgctaat aaacatgtat tgatagttgt aagaggtcag gactacaaag
    66841 aagatctaag tactatgctt tcttatatag aagaggtaaa gccagtattg gttggtgttg
    66901 acggaggagc agacgcttta attgagtttg gatatactcc agatgtgatt gtaggggata
    66961 tggatagtgt aagtgatgaa gctttaaaaa aggctagtga aatagtagta catgcttata
    67021 cagatggaag agcaccaggt cttaagaggg ttgaggaatt aggcttagat gcagttgtat
    67081 ttcctgcgcc aggaactagt gaagatatag ctatgcttat cgcttatgag tataaagctg
    67141 agttaatagt agcagttggt acacattcaa acatgattga ttttcttgaa aaaggaagaa
    67201 aaggtatggc aagtactttt ttagttagac taaaaatagg ttcaaagctt atagatgcta
    67261 aaggagttaa tttgttatat agaagtaaat taaaaataaa gtacatatgg gcattaatag
    67321 caacagcatt atttcctgtg ttggtagttg catcattgtc accaggagtt caacagttta
    67381 tacaactaat gcaattaaag tttagagtat tattacagat gtaaatttta ggaggataaa
    67441 tatgcatatt aatatgaaat attatatagt cactataggg gctatattta tagctttggg
    67501 tataggcatg ttagtaggtt ttaatttaaa taataatcaa caattaagtg agcaacaagc
    67561 aaatataata aatgatttgg atgataagtt taatatttta aaagaaaaaa atgataaact
    67621 tgacagtgac ttagccaatg taaatagaga ttatgaagag gcagttaatt ttataaataa
    67681 aaatgtagat aagattttag ctggctcatt gaatggtaaa agtataggaa taatatcggc
    67741 taatgaaaat gatgattata caaaaaatat agaagacata ataaataaat ctaatggaag
    67801 tattgcattt aatatagtgt taaaagaaaa tataacaaat ccagaaaagt taaaggaaat
    67861 gtcaactaaa ctaggtacag atgtaaaaaa tgcaaatgat gcagttaatt atataatcga
    67921 cactcttaaa aaagaagatg caagtgatat tttaacatat cttcaagaac tagatgtaat
    67981 aaagtttaac tctcttggaa atacatatct aaaatatgat tctgttgtaa tagctggtgg
    68041 aaatgatgca aaagacagta caaaacagtt tgaaaaaata gaaaaatttg tagtttcaaa
    68101 acttaaatca gaaaataaat atttagtaga agttcaaaat acaggagtta aaacttctta
    68161 tgtagaacta tattctaaaa ataaagttgc aactatagac aatattgatg aaggtatagg
    68221 aagtatatct ctagctattc ttctacaaca aggtaatata gttggtaatt ttggtagatt
    68281 agatactgca acttcattgt tgccatctat taagtagaaa taactttaag gagagaaaaa
    68341 atggattctt atataagtat aataatacca gcttataatg aagaaagtag aataaaatat
    68401 acattagaaa gcatattgga cattaatcaa ataaatgaaa taatagttgt cgatgatggt
    68461 tcgattgaca atactgctaa agctgtatct gaattagaaa atgaaaagat taaatttttt
    68521 aagttagata aaaatagagg taaaggatat gctttaaatt atggtatcaa gttagcaatg
    68581 aaaaatgcag atgttatagg ttttcttgat ggggatttag gaagctctgc taaagaagtt
    68641 gaaaaattaa tagtacctat attaaatggt gaggcggatg ttacaatagc caagtttcct
    68701 cctgcaaaaa gaaaaggagg tttgggattt gtaaaaggtt tagctaaaaa ttctgtgttt
    68761 gagatgactg gcgttgagtt agatgcaact ttatcaggtc aaagaatatt taaaagagaa
    68821 gttttagaaa agtttgatga aatacctttt ggatatggag tagaagttgg aatgaccatc
    68881 gatatcttaa agtatggatt taaaattaaa gaagtattag taaatatgac acataatgaa
    68941 acaggaagaa atttaaaagg ttttatccat agagggaaac agtattatca tataaaaaag
    69001 gttttaggac aaaaaaagaa agaatggagg taatatctta gtgaaagatt atatacttta
    69061 taccatttta ttgttaactg gattatttgg aacctatgct gtaattccac tatttaaaaa
    69121 tttattgata aatggaaatg tacttagacc aaactacaaa aaagatatga taccagtaag
    69181 tatgggaatt gtatttttac ctatgattgt aattaatgga ataatacttg ctttctttac
    69241 tacagaattt aaggatttag cgtatatatt tatgtttatg tttggtatga tatccatgtt
    69301 ttttgcaggg atattggatg atgtcattgg aaatagagat gtaagtggtt taaaagggca
    69361 ttttaaaagc ttatttaaag gaagtcttac aacaggaggt tttaaagctt tatttggtgg
    69421 atttgtaggg cttatagttt ctgttgctat atcaaaagac ataattgata taatagtaaa
    69481 tacactaata atagctttat caactaattt aatgaatctt ttggatttaa gaccagggag
    69541 agcaataaaa gcatatttat tcataatggt aattatattt ttaacattga caggatttgt
    69601 tcaagttctt ccattactta tagtaccaaa tgtacttgca tactttaact atgatttaaa
    69661 agcgagggct atgatgggag atactggttc aaacgttctc ggaatatcta ttggaatgct
    69721 catttctttt gggtatccat ttaacattag attaggatgg ttaatatttg tagttattat
    69781 gcatatattc actgagaaat actcgttaac taagataata gaaaaaaaca aactattaaa
    69841 ttttatagat aaattgggaa ggtagtatta tgataagcaa aagagtgggt attgttgaat
    69901 caatagtaag tcaaactgaa actctagatg atataagagt aaatataaat ggagaaatac
    69961 aaagggcata taattatcca aacatatctg gaactataaa tataggagat gaagtcgttt
    70021 taaatacaac agcagtagaa ctgagcttag gtacaggtgg ataccatttt gtaataacta
    70081 atttaaataa tatagaatct actttaactg aaggcggtca tataatgaaa cttagatata
    70141 cacctcttca aataaaagta gattctgttg aggaacaaga aagtatttat catgataaat
    70201 ttgcgaattt taaagggtta gaaggtcttt ctgttgtagt agggactctt catagtatgt
    70261 taactccatt tgtagcttca tttaaaagaa ataatccaaa taaaaaatta gtatatataa
    70321 tgactgatgg agcatctctt ccaatttatt taagtaaaaa tgtggatact ttaaaagaaa
    70381 aaaagctaat agatagtaca ataactatag gaaatgcgtt tggtggagat tatgaatgta
    70441 taaatatata tactgcacta attacagcaa aagaaatact aaaagcagat gtagtatttg
    70501 taagtatggg acctggaatt gctgggacag gaacaaaata tggatttaca gggattgaac
    70561 aaggctctat tcttgatgct gtaaaaaaac ttgaaggaag agcaattgca attccaagga
    70621 ttagttttgc tgataaacga gaaagacatc aaggtatttc acatcatagt atgaccgtat
    70681 ttaaagaaat tgtaaatgta aatgtagaca taccaataac tatatatgat gatgaaaaat
    70741 tgaactttat aaaagagcaa atcaatttaa atggtttaaa caaaaagcat aatataattt
    70801 atatagataa taataaaacg aaagaggatt tagactattt taatctaaaa gtaaagagta
    70861 tgggaagaaa ttatgaacaa gatgaggcat ttttcaaagc tgcaagcaca gcagcatatt
    70921 acttaatgga ggtttaagat ggtattagaa gaaaaaacta ttagtagtga tagggtttat
    70981 acaggtaaag taattacttt aaaggttgat actgttgaaa ttccagggca aggatatcaa
    71041 aaaagggagt tagtagaggt tggtggagca gtaggaatag ttgcaattac agatgataat
    71101 aaagttgtac tagttaagca atttagaaaa cctatagaaa agccaatctt tgaaattcca
    71161 gctggtaaac tagaaaaaaa tgaaagtcca aaagaatgtg ctgaaagaga attaaaagaa
    71221 gaaactgggt atagtgcaaa aaatgttaag ttaatacata agtttttcac ttcagcaggt
    71281 ttttctaatg agataatgtt tgtatattta gcaacaggtc ttactccagg agagaataat
    71341 ttagatgcag atgagttttt agatgtacat gaaattgagc ttgaagaagc atataatatg
    71401 gtactaaaaa atgatgttga agatgcaaaa acttctattg gattattatt agtaaaagat
    71461 atgtttaaaa attaatattt ataaaatgaa taaccctttg tcctaaagca tattatttta
    71521 taaaggacaa gggggaatta aattgagaag aacatataga aaaaatgatt ttagagaatt
    71581 aaatagacaa ataattatta taggattgtt gtttatgatg tctattgttg ttgggtctta
    71641 cataaataaa atattgccaa gttctagtaa caatatatta aacaatataa atcctgctgt
    71701 agagtattat aatttaaata taagcataaa agatgcagtg atacaaaatt taaaatcaga
    71761 tgcaatcttt atgggctcaa tagctctatt aagtctgttt gtagtaacaa ttccagcagt
    71821 gttggtagct tttgtattaa aagggatgtc aattggttat acaataaata gttgtatatt
    71881 ggcattaaaa tttaaaagta taaaaatgat tttaataata ttatttaaaa atttgattat
    71941 tattccatgt gcaatcatat taacattaat tagcttaagt tattttaaag aaatggttta
    72001 tgaatttaaa aagaaaaata gaaaaaatat gcaattttta attaagaggt atatactaaa
    72061 tattataatt ataatagcac tatcattggg gttgcaatta atattaaata ctgtaagtat
    72121 tggtattatt aagttcctag ctaaataaag gagtttataa tggatattat agagggatat
    72181 atagattaca taaaagataa aaaaaattat cagagaatac agtggcatca tactttatgg
    72241 atataaaaaa atatatggaa tatataaatc aaaaaagcat taaactaata gatatagtag
    72301 aaaatgatat tataagttac ttaataaagt tggaaaaaga taatgtatca atagctacaa
    72361 tagcgaggat gatatcctct ataaaatctt ttcatgatta tttattttta aatcatatat
    72421 gtacaaataa tcctgcaaag gatataaaaa aacctaaaat aaaaaaagaa aatataaata
    72481 tacttacaga agaagaaatt gaaagactat taaattttcc aaagttaaca acacctaaat
    72541 tgattaggga caaggctatt tttgaagtat tatatggtac tggtataaaa gtatctgaat
    72601 tagtagagat gaatatagaa gatattgacc tagacattga ttatatatat tgtaattcat
    72661 gttgtaaaag tcaaagagtg ataccacttt gtgatattac aaaactttat ttagaaaagt
    72721 atctaaaaga agcaagacct aaaatggctg tagaaggtga aaaatcatta ttcgtaagtt
    72781 cgttaggtca aaggtttaca cgacaaggtc tttggaaagt cataaaaaaa tattctaatc
    72841 tagccaatat cgataaaaat ataaatccta caatgcttag acattcattt gcaattcatc
    72901 tgttaaatga aggtgctaac attgctgttg taagtaaaat tttaggaaat gttaatttat
    72961 ctagtttaca agtatattta aatcatatag ataaaaacgt gagaagagaa ataaaggaaa
    73021 aacatcctag aaatgatgtt gatatagaat tacaaaaagc agaggctaaa tagactatat
    73081 agcaaatttt ataatttaga aggagcatat ttgtatgagc agagtaattt ggattgtaat
    73141 tgatagtgtt gggataggag cattgcctga tgctgaaaat tttggagata gtaaggatgt
    73201 aagtactctt ggaaatatat ttagagaata cccagatata aatattccta atatgagaaa
    73261 attgggtata ggaaatatag atggtgtaga tttttttgaa agtataaaaa ccccaatagg
    73321 ttgttttgga aagtgtaaag aaatgtctca gggaaaagat actactacag gtcattggga
    73381 aatgactggt ataatagttg acaaaccatt taagacattt gaacatggat tttccaagga
    73441 gataatagac gagtttgaga aaaaaactgg aagaaaagta gtgggaaata aaccagcttc
    73501 aggaactgta ataatagatg agtatggaga gcatcaaata aaaactggtg atgtaatagt
    73561 atatacatca gctgatagtg tatttcagat agctgctaat gaagaagtaa ttcctctaga
    73621 agaactctat aatatgtgta aaatcgctag ggaaataatg atgggggaca atgcagtagc
    73681 tagagtaatt gctagaccat tcataggtaa gaaaaaaggt gagtttgtaa gaacttcaaa
    73741 tagaagagat tattcattag acccatttga accaacagtt cttgacaata taaaagaatc
    73801 aggtcttgac gttttagcag ttggaaaaat agaagatatt tttaatggaa aaggaattac
    73861 agatgctatc cacactaaaa gtaatatgga tggagtagat gaaactttaa attatatgaa
    73921 acaagataat aaaggtctga tatattcaaa tcttgttgat tttgattcta aatatggtca
    73981 tagaagagac ccagaaggat ataagaaagc tcttgaagag tttgatagta gacttcctga
    74041 aataatggct aatatgagag aagatgatat tttaataatc aatgcagacc atggaaatga
    74101 cccaacatat aaaggtacag accatacaag agaatatata ccagttatga tttatggcaa
    74161 taagattaaa aaaggtttta atttaggagt aaaagatact tttgctgata ttggagcaac
    74221 tgttgcagat atactaaatg taaagttgcc taaacatggg tcaagcttta aaaaagattt
    74281 attttaaatt agaaaataac aaaaacttag tttaatagga tggagagatt ggaatgtttg
    74341 aaaagatagc acaaagtagt aaatttataa atgctaaaat taatataaaa cctaaaattg
    74401 gtttaatatt aggttctgga ctaggcgatt tagcaaatga tatagaagac tctgtcacaa
    74461 ttaaatatag tgaaattcca aacttcccta tatcaacagt agcaggacat gctggacaac
    74521 ttgtcattgg aaaacttgaa ggaaaagaag ttattgctat gcaaggtaga tttcattatt
    74581 atgaaggata ttctcaaaaa gaagctactt ttccagtaag ggttatgaaa gaattaggag
    74641 ttgaggttct tatagtaacc aacgctgctg gaggtgtaaa taaagaattt aaagcaggag
    74701 atttgatgct tataagagac catataaact ttagtggaag taatccatta gtaggaaaaa
    74761 atgatgacag acttggtgat agatttccag atatgtcgga tgcatattct tctagatatg
    74821 tagatgtagt taaaaactgt gctaaagaat gtaatataga tgtcaaagaa ggcgtttata
    74881 tgttttttag tggtccaaac tatgagacac cagcagaagt gagaatggca caaattttag
    74941 gtgctgatgc tgtaggtatg tctactgttc cagaagtcat agtagcttca cactctaata
    75001 taggagttat tggtatctcc tgtataacaa atatggctgc tggcatctta aatcagtcac
    75061 taagtcatga ggaagtaata gaaactactc aaaaagttaa atcagaattt atgaatttag
    75121 ttaaaacaac agttaaaaat ttataagttg aagattatta taaatttttt gagcaaattt
    75181 ataataaaat agttaatgtt taatttaaaa ggattaataa agagctgtct tgatatagaa
    75241 agttatttct aataagggac agttttttta taatactaca acatttgaaa gtgttttaaa
    75301 gtttaatatt tatttaaatt attataggtt agaatggaga tttcaattat gagaatgtat
    75361 gatattataa gaaaaaagag agataaccta gagcttagta aaaaagagat aaactacttt
    75421 atagaaaatt attcaaaggg agatatacct gactatcaag cttctgcact attaatgtct
    75481 atttatttga ataagatgaa taaacaggaa actgtacatc ttacagaagc aatgatgaac
    75541 tctggagaca tgataaactt atctgagata gatggaataa aagtagataa gcatagtaca
    75601 ggtggtgttg gagataagac tactatagct cttatacctt tggttgcttc atgtggagca
    75661 ccagttgcaa agatgtcagg tagaggtctt ggtcatactg gtggtacgat tgataaattg
    75721 gagtctatac caggtttctc gacagaaatg gagataagta aatttattaa ttctgtaaat
    75781 aagtctaaaa tagcggtttg tggtcaaagt gcaaaggtag caattgcaga taagaaaata
    75841 tatgctctta gagatgttac agcaactgtt gataacatat cattgatagc atcaagtgta
    75901 atgtctaaaa aattagcttc aggagctgat gcaattgtgt tagatgtaaa aactggtgat
    75961 ggagcattta tgaagacgtt agaagagtct tttgatttag caaagtctat ggttgatata
    76021 ggcactggta tgaatagaga tacaataggg attgtaacag atatggatga acctttaggt
    76081 tttgcagtag gaaactcttt ggaagttata gaagcaattg agactttaaa aggaaatggt
    76141 cctaaagacc ttgttgagct atgtgaagta ctaggagcat atatgctggt tttatcaaag
    76201 gtagcatatg attttgatga tggtgttgaa aagataagag aatcgataaa atctggtgac
    76261 gccatacata aactcaaaga gtttatagag aaccaaggtg gtaataaaga tgtagtagat
    76321 gattattctt tattcaaaaa atcaaagtat aaatatccag ttaaatcaac taaatcaggt
    76381 tatataaata aaattaaagc tgaagatgtt ggtgtatcag ctatgattct tggggcaggt
    76441 agagaaacta aagatgatgt tttagattta tcagtgggga taattttaga aaagaaagta
    76501 ggagattata taaatgaagg tgaagtttta gcgtacatgt attataatga tgaacaaaaa
    76561 ctttcattgg caaaggacag atttattgat tcctattcta ttgtagattc gaaggtagaa
    76621 aaaaataaac taatatacgg tattgtaact aaagaaggga ttaaaaagtt ttagaaaatt
    76681 ttagaaaagt gattataaaa ggcctaacat gtcaaaaata atgttaggtt ttttattttt
    76741 tgataagttg taaattatac caaaaatata tattttaaaa cattttttac aatataaata
    76801 agtttattct acaagatgat tgaaaattaa agtatctagc tttataatag aaaatggata
    76861 aatatacata aggagtagat tatattatga actttaaaga aaatagattg aaaatagcag
    76921 cattaataat tgttattttg attatcttag caggtatatt tgtgtctatg caaataggtc
    76981 cttataataa aaataacaaa aaagatgtaa ttattgacat tccaaatgga gcatctattg
    77041 gaaaaatatc tgatatatta tatgaaaata aattaataaa aaatgaacta ttatttaaac
    77101 ttttggtaaa ggttagcaat aaagctccat ctataaaatc aggaacatac ttacttaatc
    77161 aatcgtactc aaataatgat ataataagtc ttttagtttc aggtaaaata tatcaggatg
    77221 gtataaaagt gactattcca gagggtgcta catctaaaga aattatagca atgttagtaa
    77281 gtaaaaattt aggagataaa gctacttttg aaaatttaat aaaaaaacct caagaatttt
    77341 atgataaatt tccatactta aaggaagatg gaataacttc tttagaaggg ttcctgtatc
    77401 cagaaactta ttattttaat tcaaagaaac aatcagaaga agatatttta agcgaaatgt
    77461 taaaggtatt tgacagtaaa tacacggaca agtttaaaaa gaaacaaaaa aaattaaata
    77521 tgactcttca agaagtaatg gaaatggcat caataattga aaaagaagct gttcttgaca
    77581 aagatagacc aataatagca agtgtgtttt ataatagact taaggtaggt atgcctcttc
    77641 aatcagatgc aacaatacaa tatatatttg aagagagaaa gaaaattgta acgtatgatg
    77701 atttaaaaat tgattcacca tataatagtt ataaaaataa gggattacct cctacaccaa
    77761 tatcaaatcc cggtataaaa tctattgaag ccgcgttata tccagataaa acagactatt
    77821 tatattttgt agctaagatt gatggtggaa ataattatag tacaaactat caagaccatt
    77881 taaaatatgt taaagaatat aaagaagcaa gggataaaca atcaaaagat acaaaagcaa
    77941 caaataaaga aaatacaaaa aaataaacca tgattataaa aagcctaaca gggtaagaat
    78001 aatgttgggc ttttttattg aaaattgtta ttcttagatt tataataaag aaagtgtaaa
    78061 tatatacaac aatatgaatt taaaaaataa taaattaata taaaacttga acatttatta
    78121 ttttagggag tgtttttatg agtaatatag ttaatgattt ggtcgaagat tatataagag
    78181 tgacattaaa agaaaaagaa ggatttttaa aagatttaga aatttatgca gaggaaaata
    78241 gtgttcctat aatacataaa gaagtttcag atttattaaa agtcttatta aagattcaaa
    78301 aaccaaagag agttttagaa gtaggatgtg caattggtta ttcttctatt ttttttgcaa
    78361 ctattatagg aaaagatgca gacataataa ctgttgagag aaatgaaaaa atgatagaaa
    78421 aagcaaaaga aaacataaaa ttagctggat ttgaaaataa cataactata ctagaaggag
    78481 atgcagaaga aaaacttagt caagtccaag gtgaatttga ccttatattt atagatgcag
    78541 ccaaaggtca atataagcta ttttttgatt tagttataga taaattaaaa gatggaggac
    78601 ttttagtatc tgataatatt ttatataagg gtatggttgc tcatgatgat tttgttgtaa
    78661 gaagaaaaaa gactatagtt aaaagaatga gaaattattt agattatata tgtaattgtg
    78721 attatttaag cacatcattg ataccaatag gtgatggtgt agcactaagt tataaagaaa
    78781 gtaaaagagg tgatgtagat ggaattaaat aaagtagaac ttttggctcc agcaggtgat
    78841 ttggagagat tgaaaatagc tataacatat ggagcagatg ctgtttatat aggtggagaa
    78901 atttttggaa tgaggtctgc agcgaagaat tttagtaaag aagatatggc agaaggagta
    78961 gcttttgcac atgaaagagg aaagaaagta tttgtaacag taaatataat acctcataat
    79021 gaagattttc tacaattaga agactatttg ttggaattag aagagatagg aatagatgca
    79081 gttataatag ctgacccagg agtacttagt gttattaaaa aagtaattcc taatatggaa
    79141 atacatttaa gtacacaagc aaatacgacc aattatctaa gtgcgaactt ctggtacgag
    79201 catggaataa aaagggttgt agtggcaaga gaattatctt ttgatgaaat atctgagata
    79261 agagcaaaaa cacctttaga catggatata gaagcgttta tgcatggtgc tatgtgtatt
    79321 tcttattctg gtagatgctt aataagcaac tatatgactg gaagagatgc aaacaaaggt
    79381 tcttgcgctc aatcttgtag atggcaatac cacttagttg aagaaaaaag acctggtgag
    79441 tatttcccaa tatatgaaga tgaaagaggt acattcttct ttaactcaaa agatttatgt
    79501 atgatagagt atataccaga gttaatcaaa tctggtataa caagtttaaa aatagaaggt
    79561 agaatgaaga ctgcatacta tgtagctact gttgtaagag catacagaat ggcaatagat
    79621 gaattttaca aagacccaga aaactggaag ttcaatccaa tgtggatgga agaactaaaa
    79681 aaaggtagtc atagacattt tacatcaggt ttttacttga ataaaccaac aacagaagac
    79741 caaaattatc aatcagcatc atatgttaga aactatgatt ttatagggat agttagagaa
    79801 acagaagatg aagatggtct tattgttgtt gagcaaagaa ataaaatgtg tgttggtgat
    79861 gaaatagagg tcatgggacc atataaagaa actatgttta ctaaaattga agcaatgtac
    79921 aatgaagaag gagaagctat agaatcggct cctcatccta gacaaatagt gaagttaaaa
    79981 ctatcagtaa aagttggtaa agattatatg cttagaaaag ttatagaaga aaaggttgaa
    80041 gaataataaa aaattgataa aaatagtatt agccccttag aaattgtcaa taatgaaatt
    80101 atctaagggg tgatttcatg tcaaaaaaga agactccatt tttgaaaaaa gtgggaaaga
    80161 gaagctggtg tgtatttact ataattttaa taatttatag tgctttaatt tataggctag
    80221 ttgatataca agtattaaaa ggtgataaat acaaacaaag tgttgaatca caaagtgttg
    80281 agaaagtcga gctaaacagt ggaagaggaa taatatatga tagaaacaac aagaagctta
    80341 cagatacaag taagtatcag gtattagttg ttgaaaaaga aaaattaaat aataactata
    80401 aaattttgga gcttattaag aaagctacta aaatgaatga tttagatata tataaagctg
    80461 tacaagagca attgacacgc cctataatac agattcaggc taaaaatatc gataaaaata
    80521 tgaaaaaaga acttgagaaa aatggaataa tgatagaaga aaaaactatg agatattcaa
    80581 aagatggttt attatcacac acaataggat atataaaaga agatgataaa tcaggtcagt
    80641 caggaatcga aaaaagtatg gacagtgtac ttagaaattc aaatgaaaag tatataagtg
    80701 cttttaaagc tggagatgca ggaaatgaaa aatctctaaa catactaaag ggaagtgtaa
    80761 aaactattga taataaagat aaagatagac atctaaaaac tactattgat tataatattc
    80821 aaaggaaatt agaacaaata ttaaataaag aagaaaatcc tacagcagca ataatctcgg
    80881 aggtatctac aggtgaaatt cttgcaatgt gttctagacc taattttgac caaaatgata
    80941 tatcaaaaag tttaaaagga aaaaatggtg aatttgaaaa tagagttata aaggcgacat
    81001 atccaccagg gtctgtattt aagatggtag tcttattttc tgcacttgaa aatggtgtta
    81061 tagatgaaaa ctatacgtac aactgtacag gcaagactaa agttgggaat actaatgaaa
    81121 tactacgatg taataaaaga gatggtcatg gtttccaaaa tttaagacaa gctttttcta
    81181 attcatgtaa tcctgcattt ttagatatag ctatgaagct tggaaaagaa aagatactta
    81241 aatctgctga aaagttgcat ttatttgaaa aggttgatat tggtcttgat gaagaaaaaa
    81301 ttagagaagc tccaaaaaac atatcaataa gaaatttagc tataggtcaa gaaaatatag
    81361 aatttacacc acttcaaata aatcaaatga ctcaaataat ggcgaacaat ggaacattca
    81421 aaccattata cttatataag agccttgtgg ataatgatat gaatacaata aaaacttata
    81481 aatcctcaaa aaaggaagaa cttatttctc catatgtatg tacacaagtt aaagaatata
    81541 tgaaatctgt gtctaggact ggtacagcta aggacctaaa agatattgag ggtggatgtg
    81601 gggttaaaac tggtacagct caaagtagtt tgaataaaaa agcaatagac catggatgga
    81661 taactggatt ttatcctgaa gaaagaccta agtatgtaat aactgtatta gtagaaggaa
    81721 ctcaaaaagg aaataaatct gcaacaccta tatttaagga aatatgtgaa agtatacaat
    81781 aaaattatat actataaaat ttaaggatat atcgtaatct tgattttaga tagaaagttt
    81841 tgtctagtga tgtacgatat atcctttaaa aatgtagtat taactttctt gaacaagctc
    81901 tttttctagt tttttaagag cttttttctc gattctagat acatatgacc tcgatatatc
    81961 gagtttttgt gcaatttctc tttgtgtctt atagccataa ggagttaacc catatcttaa
    82021 ctggacaatt tctctttctc taggagtaag aattttgttt agttgttcgt aaagctttcc
    82081 aacttgaacc tttagctcaa cttcatcaag aacatagtta gcttctgtgc caagtatat
//
LOCUS       DAFNNB010000002        76426 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0002, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000002 DAFNNB010000000
VERSION     DAFNNB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 76426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76426
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0002"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(208..651)
                     /locus_tag="L3M60_000086"
     CDS             complement(208..651)
                     /locus_tag="L3M60_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224796.1"
                     /translation="MKKNMLYLGIVYFICGILLVLLATFTEFSFEAFIWGLSGAALGP
                     GVCMILQYMYWSKPERALDYEEKIKNQRIEMNDERRIMLRDKAGRITNQIMSYVLVIL
                     IFIVSILSVFSVMVISKWVLVVLGLLILFQFICSVVVYNKLDKKL"
     gene            complement(654..851)
                     /locus_tag="L3M60_000087"
     CDS             complement(654..851)
                     /locus_tag="L3M60_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6224797.1"
                     /translation="MKNRLEEIRKERGIKQEELASVLQVSRQTIGSLENGRYNPSIIL
                     AFKIARYFDRSIEEIFIYEEE"
     gene            complement(1043..1909)
                     /locus_tag="L3M60_000088"
     CDS             complement(1043..1909)
                     /locus_tag="L3M60_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6224798.1"
                     /translation="MEWINKLNQAITYIEENLESGIDYAEAAKIACCSTFHFQRMFSY
                     IAEVPVSEYIRRRRMTKAAFELQNSNIKILELSEKYGYDSPTSFNRAFQNIHNISPSA
                     ARAKGVVLKAYPKMTLSIYVKGNVEMQYRIVEKKGFRIVGIKESMNMIVEECFEKVPK
                     LWAKCIKNGTIDKLSELINNEPCGLLGVSVCTNSKGLDYYIAAPTDKPILEDTHEYLI
                     PSGTWAVFECVSPMPNALAIQELQKRIITEWLPSSGYVYSNLPDIELYPNGDINSPDY
                     TTEVWIPIQKPS"
     gene            complement(2134..3003)
                     /locus_tag="L3M60_000089"
     CDS             complement(2134..3003)
                     /locus_tag="L3M60_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6224799.1"
                     /translation="MDYKTIILNRKSVREYKNTDIKEEYLSEIKKYSESCKKLVPEID
                     VDIRIMNRSEVFNNLEKVAGYKGNMIDSPSYIIILSDVKDNYIENSGYIGENIIFKVT
                     ELGIGSCWVTFEDGKKVKEKLGIDSDKEVTGIIAIGYGENVNNAKVLNATKTGQNYSK
                     SDMQVVTDKGSSRLGVEDVVYMNEWGNVASIEVLEERALLDAFHYARLAPSTLNRQPW
                     RFIVDGGTVILAVRKDGHTNLYEEKIDIGIVMLYFATIISATMFELKWNLGTPDKDYK
                     VPEDYKIVGYCNI"
     gene            complement(3509..3730)
                     /locus_tag="L3M60_000090"
     CDS             complement(3509..3730)
                     /locus_tag="L3M60_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224800.1"
                     /translation="MNVNNHKCPECGEHSKKFEIVEEHEDCVIFECYTCGTISILKYC
                     ENCKEFVFVNIPNNVETYEYCCGKCNNKI"
     gene            complement(4231..5211)
                     /locus_tag="L3M60_000091"
     CDS             complement(4231..5211)
                     /locus_tag="L3M60_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224801.1"
                     /translation="MGDTSRFYESVRNKYPKLKDGTRVHLWPKENIIIGPIYDEEDGY
                     KKSIGNVDILSWKQIVVLAECDGKNTTDDIINILSNRYRNIPNMREKVMEFFMFYENV
                     YLTFEDNIQTVSSVFEITGNKSYITPLYFTLEIDADNNTNNYFSEITSLLKCMYEKGC
                     RFIEVIGEDILKNKGMRDVFQYMLDHFDLIVVTKDSFTIDRSLIKELDNYRHKVIWKV
                     YSRNDDTRIKLKEDIKITSLIKRGHTVVRRDREKAIKIEDMRFKDTKERFSKYGAEWS
                     HLYISSNGNVKSYSFQKDKRVYLGNIFNNSVEEIFLEMQNKIDKLEIVYQ"
     gene            complement(5375..6247)
                     /locus_tag="L3M60_000092"
     CDS             complement(5375..6247)
                     /locus_tag="L3M60_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBN6224802.1"
                     /translation="MIIKVSMTVLVLILLYILYKYETQHIETTEYTIFNKKIPVEFDN
                     FKIVQVSDLHNKVFDKNNKVLMDKIKSLNPDVIFITGDLVDGENKNFQVALDLIDNLV
                     EKYEVYHIIGNHEQKSLIKKYKHLYKDYFKELYSKKIINLENESVRIYKGNSHLNLYG
                     LIVPLECYPYFFANNKKLELSDNFVESNLGKVNENEYNILLAHTPFFFEKYAKWGADL
                     VLAGHVHGGIIRMPFVGGVLSPNREFFPKYDWGRYEKDNSTMILNKGLGGSKVLIRLN
                     CKPEIVKITLKSKK"
     gene            complement(6411..6962)
                     /locus_tag="L3M60_000093"
     CDS             complement(6411..6962)
                     /locus_tag="L3M60_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6224803.1"
                     /translation="MDNKFPNVGENLRLLRQEMGISLDKASKMTGVSKAMLGQIERGE
                     SSPTVSTLWKISSGFKINFTTLLNENTNTYEVIKKEEVEPIVEQQGNMKLYPIYPFSP
                     QRRFELFIIELEENCTHVSSTHSHVLEEYVLVIEGKLDLNVDGKTYILESGHSIRFDG
                     TLEHIYKNLNKGKTIFHNIEVYR"
     gene            complement(6982..7884)
                     /locus_tag="L3M60_000094"
     CDS             complement(6982..7884)
                     /locus_tag="L3M60_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBN6224804.1"
                     /translation="MSKKGRVVKVQHFSVNDGDGIRTTIFLEGCKLKCKWCSNPDSWS
                     NIVKLGVMKDKCISCNRCVDVCPQNISSLFDRTQINNKCDLCGECIKACLKDAICIMT
                     EEMSVEEIVKEVEKDFIFFFESNGGITFSGGEPTLQIDFLRELVDIFYNKGINIAIET
                     CGYFDWNKVNDVFDKIDHIFVDIKSMDDNIHKEYTGVSNKIILDNICRLSKLNKSMVI
                     RVPIIYGVNDSEENIRNTALFVKQNVPEGKMELLPYHKFGIDKYKALGLEDYIYEFDE
                     ICNNHMLKLKEIVELTGVKIIEYK"
     gene            complement(7899..10271)
                     /locus_tag="L3M60_000095"
     CDS             complement(7899..10271)
                     /locus_tag="L3M60_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase/glycerol dehydratase
                     family glycyl radical enzyme"
                     /protein_id="HBN6224805.1"
                     /translation="MSQTNRIEAFRQEYINSKPMICCERARIFTESHKKTEGEAICIR
                     RAKAFLETCKELPIKIFENELIVGTAGKFRRTGILTPEFSWQWVDKEMDTFDERTQDP
                     YVISKEQIEFIRKEIFPYWKGKSLEEVFLARIPEDTAKVLVDTGIIDNDSKWRQAVGE
                     VTPDYQDILFVKGYKGIKEDADKKIKELDISISENIEKIDFYKSVSIVAQGIMTLAQR
                     YSNLAKEMSEQETDKKRKLELIKISEICMNVPANPPTNFYEAIQFVWFVQLGGILSEN
                     PLALNLGRFDQYMYPYYENDAREGKITESEAQELIEALWIKLSEWVWTISANTANYFA
                     GYNQFQNLTVGGKKRNGTDGTNDISYMCLKATESVKTHQPGLSVRVSQGAPDNFVMAV
                     AKLVKQGTGFPAIHSDNAGAQMLLQDGYDAEDARDWSNCGCVVPHFRKTGQWTSAVNI
                     NFAAALEYAMNEGKSRLTGEKMGLDTKNITEFTSFEELKDEFLNQLAYLVKNSVIGTT
                     VAQQIHKEMVPRPFLSTCVDGCLDKGVDLSKGGAKYNIGPVLTGIGLGVVSNSLAAIK
                     KLVFEDKVTTLEELTKALNNDWEGYEELRKLALDVPKYGNDNDYVDSLAIEVSDFYYT
                     ETRKYKDIFGSKFNSAFMGISNYVPTGKIVGATPCGRKATKPLTEGVSPFVGTDTTSP
                     LAAMKSASKINHDVHTGGTLLNLRLNQDLVETERGLRNLTSMVKSYFALGGFHVQFNT
                     ISNDTLLKAQENPEEYKDLLVRVAGYSTQFVNLSREMQDAIIARNSHSNF"
     gene            complement(10492..11196)
                     /locus_tag="L3M60_000096"
     CDS             complement(10492..11196)
                     /locus_tag="L3M60_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224806.1"
                     /translation="MGGLCMKVKRNINKVAIVVALNLSLALMVGCKSPVSDKNLSSSK
                     QISKDSLKESVEDKKDESIELFNNYSQKMNDVKGREEVKKDLSQRFSKAIIDSGYSIT
                     NSKSDGSFVVSINSVEKPPEENIRSLFYSVSEDDKEGKVTIDILCAKEYPEDEKLSES
                     DKYIKFIYNLFKSLTNTNLTEKEIFSMVEEDFNKGVGVVELPYMKDIHVEVNKIKQST
                     KVLKLSLLYKFDISNR"
     gene            complement(11422..12801)
                     /locus_tag="L3M60_000097"
     CDS             complement(11422..12801)
                     /locus_tag="L3M60_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6224807.1"
                     /translation="MLNFMTELLVVLSIFIQFSIMNGILIYKKDSKKNEKIVFYIVLF
                     LIYIIVQISRLFNGGNYFSNINYLFYILAFIAWGGYYNYFYKIGYIKYSVFFYLFSVY
                     YECLDAVIYRILFFAITGNVISSKESLLIPHLESIKIQIYSNLIIILIYLIFSFFRKS
                     IKLISQDKRNYVYLLFALLANTMNMLVNYVLKKLERFGSLHSEGYYFDNFVNPKLVGA
                     SSIFLILLFKEIIKENRLKSQAELIKNKLDMQYAHYLSIQESHMRVKKLYHDINNHIY
                     CIDKLRNNSKEIDEYVNSLKDEIKAFKYIYNTGNMILDIIINEKSEICLKKGIKFICS
                     INFSKVNFVKPIDVSSIFSNVLDNAIEACDKILDENINKYIRIKGTITKSFFVLKCEN
                     SKLNKITFNKNILLTNKMDKFVHGIGIQSIKSSLQKYNGELSFENSMDKFILNIYIPL
                     DQDTDSWSD"
     gene            complement(12795..13508)
                     /gene="rgbR"
                     /locus_tag="L3M60_000098"
     CDS             complement(12795..13508)
                     /gene="rgbR"
                     /locus_tag="L3M60_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgbR"
                     /protein_id="HBN6224808.1"
                     /translation="MIKIAVCEDEKETQLLIEDYLENILEDINIEYEIQKYLSGEDLL
                     ESNLKDIDILLLDIKMEKLNGMDTARKIREVDNEMEIIFITSLIDYVQEGYEVRAYRY
                     LLKPIELEDLKKHVLTCIKDIEINKKSHITIKNKSNTYKIYLNEIKYIEVQKKDMLIH
                     TINKNFDIKYSLSKIEKELNPYKFIRCHKSFIVNLRYVENIKPNTVILESGEEVPISR
                     YRYKEVKEKFLKFLGDTIC"
     gene            complement(13755..14315)
                     /locus_tag="L3M60_000099"
     CDS             complement(13755..14315)
                     /locus_tag="L3M60_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HBN6224809.1"
                     /translation="MSVMEKKIKFKFDWYSSPDKIEKWLEDIEAKGYNLYRINKFGGI
                     FYFHKESPRKIKYTVDYYYDAENNYFDEHKSNGWNLVFRSIGSFMGTSAWYIWSKEYK
                     DIPPKLYSDSDKKNKVKSILFMNMFIFFMLVVIFIYLLNIEIRNLYSGCGASVTAIVL
                     QIILLILYAVQLYRRLMYYLRLKKFK"
     gene            complement(14411..15214)
                     /gene="zupT"
                     /locus_tag="L3M60_000100"
     CDS             complement(14411..15214)
                     /gene="zupT"
                     /locus_tag="L3M60_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBN6224810.1"
                     /translation="MNNVIFAFLITLLAGLSTGIGSCIAFFAKKTNTKFLSISLGFSA
                     GVMIYVSMIEIFPKAQDALTKSMGEKLGGWSTVIAFFIGMAIIAVIDKLIPQEENPHE
                     IKKMENIEEKNIKKNKSLLRTGVFTAMAIAIHNFPEGLATFISALDDVTIAIPIAIAI
                     AIHNIPEGISVSVPVYYATGDKKKAFYYSFLSGMSEPLGAIIGYVLLRNFLNDITLGI
                     VFAIVGGIMVFISLDELLPSAREYGEHHLSIYGLIAGMGVMAISLLLFK"
     gene            complement(15365..16528)
                     /locus_tag="L3M60_000101"
     CDS             complement(15365..16528)
                     /locus_tag="L3M60_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6224811.1"
                     /translation="MDIKEITKSYKDYVIKLRREFHENPEKSMEEVRTSKRVKEELDK
                     MGIPYVSAGGTGVIATIKGANSGKTVALRGDMDALQVVECTEVEYKSKNEGLMHACGH
                     DGHTSMLLGAAKVLNDIKDSINGTVKLFFQPGEEVGKGARAMIQDGAMEGVDSVFGIH
                     LWTDVESGTISVEEGPRMASADFFKITVKGRGGHGSLPHQGVDAVLASSAIVMNLQSM
                     VSREVSPLEPLVVSVGVLNSGTRFNVIASEAVLEGTIRLFNPELRKKIPGILERIAKS
                     TAGAYRAEAELEYGYLTPAVINDKECSKIATEAAIKLFGEDCITLFEKVTGAEDLAEF
                     MNIAPGALAFVGARNESKGACYPHHHGCFNIDEDALEIGTALYVQYAVDFLNK"
     gene            complement(16588..17775)
                     /locus_tag="L3M60_000102"
     CDS             complement(16588..17775)
                     /locus_tag="L3M60_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224812.1"
                     /translation="MEAITAFCLVLISLTIGDVVSAKTKAFVPSVFVSAIIFIVGFWT
                     FFPENIVDLAALGTPLAQLGMLLLITHMGTMMSIKELAGQWKTIVIALAGIVGICVGA
                     LALGTVVFGWDTAVIATPPLTGGLVASIMMADAATAKGLASLSVLAILMYVAQGFAGY
                     PITALMLKKEGKRLLSDFRSGKVTVNTAEEKVKDLPEQKSRFKIIPDLPEKYDTTYMI
                     ILRLGFVAWLAVQFTNLTNEVVSRYVMCLIFGVIASELGIVDRKPLNKSGSFGWLMTS
                     LMAFIFAGLAKATPAMLVEMAVPLVGIIIFGVIGMGIFSIIAGKLLGESKEMAFSVSL
                     TSLYGFPPNYILTEEAVKALAETPEEKEFLMDKMLPKMLVGGFTTVTIASVVIAGIFV
                     NLI"
     gene            complement(18138..19838)
                     /locus_tag="L3M60_000103"
     CDS             complement(18138..19838)
                     /locus_tag="L3M60_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6224813.1"
                     /translation="MIKQFVKYYKPYKKIFTLDLIAAFLFSLCDLVYPMITRNIMDDV
                     VPNKNLRMLVVFAVALILIFIAKAGLNYFMQYWGHVIGVDMQADMRNEVFTHLQRLPN
                     TYFDNNKSGVTMSRIVNDLMDITELAHHGPEDLFISIVMLVGSFFILIDINIPLTLII
                     FAILPFIIWFAIAKKDKMNIAFMKSRVTIGDVNATLENSIAGMKVTKSFCTEKEELNK
                     FVRSNKLFRRARQDSYKVMAEYYSGMNLYMDILEWVVVIAGGYFTYIGKITLGDFAAY
                     ILYVKMFIQPMKKLINFTEQYQNGMTGFKRFIEIMEQDHQKEAKNPIELENVKGDIEI
                     ENISFTYEDKTQVLDNLSLSIKAGKTIALVGPSGGGKTTLCNLLPRFYEFDKGDIKID
                     GKSIRDVSLKSLRKNIGIVQQDVFLFTGTIRDNILCGNPNATDEEMIAAAKKARIHDF
                     VETLPEGYDTYIGERGVKLSGGQKQRISISRIFLKNPPIIILDEATSALDNVTEREIQ
                     ESLEELSKDRTNLVVAHRLTTIKNADEIIVLTDKGIEERGTHEELVNKNGVYSRLHNN
                     "
     gene            complement(20078..20353)
                     /locus_tag="L3M60_000104"
     CDS             complement(20078..20353)
                     /locus_tag="L3M60_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6224814.1"
                     /translation="MKKILVACGAGIATSTIVCDRVERLVKENNIQAEIEQCKIAECS
                     TKQEGADLIVSTTILPTTYDIPTIKATGYITGINTTALDKKILNALK"
     gene            complement(20472..21023)
                     /gene="mobB"
                     /locus_tag="L3M60_000105"
     CDS             complement(20472..21023)
                     /gene="mobB"
                     /locus_tag="L3M60_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBN6224815.1"
                     /translation="MKGHNFMYNILSVVSYSGVGKTTLIEGIIKELNKKGYRVGVIKH
                     TCHDFDMDEEGKDTYKHRKSGARKVCIISDKRVAYIEELKDKSPLYKMIQLYEDMDLI
                     IIEGYKNYRFKRLEVTRKDKYEDIFSNKSDLVGIVSDIKYNLNIKQFDLNDYKNISKY
                     IESCIKNDILSISNVEIKNILKE"
     gene            complement(21028..22209)
                     /locus_tag="L3M60_000106"
     CDS             complement(21028..22209)
                     /locus_tag="L3M60_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBN6224816.1"
                     /translation="MIELEDAIKIIEKNVLPINRVKRVNLIDAVNKVVAEDIYSTIDS
                     PPFDRSPYDGYAYNANSENRNLKVIGTLYAGEVFNRVLNKGEAVKIMTGGKIPRGANC
                     VIKKEDVTVIGDMITLNVKLKEYDNYIFKGEDIKKGQLIINKNQNIGYDGIGVLASLG
                     ISKVTVYDDLKVGILNTGTELQDINETLENGKIYDSNRYTLYSRLLKLGIQADTICNC
                     SDDILELEKKVRDMLQNADFLITTGGVSVGDKDLIPDVFKKIGATELFWKINIKPGGA
                     CYVATLGEKLLFGLSGNPHAAIIVFDNVVVPVIEYLTYKKGIHYIKAIFKGEFNKLSN
                     KDRFVNGKLYTSEGKLYVELNDKKDAKARLSATLRSNCMIKIKKNETIKENQLVDIVI
                     R"
     gene            complement(22421..23155)
                     /locus_tag="L3M60_000107"
     CDS             complement(22421..23155)
                     /locus_tag="L3M60_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224817.1"
                     /translation="MKLKRSLVCVTILGIILVGCHKENTKEKNQVASKATQQKTMTKV
                     QNDVNEIMNKDYKYIIKNMGIPYNTFYYIKPKVLKESNTMQDINTSSYMTLVYPKYTG
                     NDELDGSALYVDINGNKVVNVETNSFSSQGIAVVDAESSIVIEKSDHEKSAVSLENFR
                     RIDLGEYVGVEESRITEIVGDANYDLTAYNHEGSKVVKSYRLKEDNKILKKEVLTISI
                     VDNKIKSIKTIESDKIVKIIKGTLLE"
     gene            23517..24422
                     /locus_tag="L3M60_000108"
     CDS             23517..24422
                     /locus_tag="L3M60_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6224818.1"
                     /translation="MDFKQLEVFVAVAKHQSFSKAARELFLTQPTVSAHIQNLERELE
                     TVLINRSNKVITLTKSGEILYEHAIYILNNCKRAIYDIKEYSGKIEGIIDIACSSIPE
                     TYILPDFMKSFSMSYPDVKFSISHYDSQYAISEILNERISFGLVGSKINNPQIEYLDL
                     LDDELVLITPSDFKIDNKNNCIDIGELAYLNFIMRKEGSGTRNLILNTLSKNNFPVNK
                     LNVIAHVESNEAIKEMVRLGLGVSFISYISAIDYLNAGKIKCYKIKDVDFTRKFFFIY
                     SKKKTFSPLEDKFLNRLCEYFEIII"
     gene            complement(24582..25424)
                     /locus_tag="L3M60_000109"
     CDS             complement(24582..25424)
                     /locus_tag="L3M60_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBN6224819.1"
                     /translation="MTTSSKKLETISPDSKITRKDFWKCFRRSLTLDSSWNYERMQNI
                     AYAYMMAPIIRRLYKDDKEKKSKALKRHLEFMSVTPHISTLLVGISGAMEEENAKNKE
                     FDANSINAVKSSLMGPVSGIGDSFFWGTLKLIAAGVGIALASQGNIMGPILFLLIINV
                     PHFIIRYICLDKGFKYGTQFFKDVSGSSIVSKVMEAASMLGLMVIGGMTASNVMLKLS
                     VNVGSGEWAEPIQTYLDQIMPCMLPAMIFGIMYWLLGKKVKTTTILISVMIICIVLAA
                     IGVV"
     gene            complement(25408..26166)
                     /locus_tag="L3M60_000110"
     CDS             complement(25408..26166)
                     /locus_tag="L3M60_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6224820.1"
                     /translation="MEFTQVILITLIAFFAYMHSFVGSTMHNRPIVVAPLVGLALGNL
                     HTGIVIGSTLELVFMGAFPVGASNPPDFVSGSIIATAFVILTGQDVSAAVVLAVPIAT
                     LVLLIDNFLMTVVLTWGAHLADRYAEEGNIEGVERIQLLFGIGNKLILAIIVGIGFSL
                     GVPVIEKILSFIPSYVTHGMDVAAGVIPAIGFAMLARMMLNKKTVAFLLLGFILVAYL
                     NITVTGVALFGLAIALIYVNFVGEKEVIVDDNEF"
     gene            complement(26183..26653)
                     /locus_tag="L3M60_000111"
     CDS             complement(26183..26653)
                     /locus_tag="L3M60_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6224821.1"
                     /translation="MIKLVRVDHRLIHGQVAFTWTKFLSTDCILIASDELLKDELRMA
                     GLKMAKPSNVKLVMKSIADSIKALNSGVTDKYNLLILCESVEDVYRLAKEVKAIKSIN
                     LGGTKSDDNRENISKAVHVSKDDINMIKELDSEGVNVFVQLVPDDDATNVMKLI"
     gene            complement(26653..27396)
                     /locus_tag="L3M60_000112"
     CDS             complement(26653..27396)
                     /locus_tag="L3M60_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane biogenesis protein"
                     /protein_id="HBN6224822.1"
                     /translation="MKKKGILSVFKSKKPIIAMIHLKGDTPEDIFERAKKEIAIFEEN
                     GVDGIMLENYYGNYYDLERILEYVSKANLSIPYGVNCLNVDTMGFELATKYNASYIQV
                     DSVVGHVKPRDEATLEEFFKLQRSKCPAYLIGGVRFKYQPVLSENNIEEDLKIGMTRC
                     DAIAVTENATGQETSMEKIELFRKNLGDFPLVIAAGVTLENAKKQLELGDMAIIGSYF
                     KDNYKDFGDVSVEHVKTFMDEIKKIREEL"
     gene            complement(27400..27813)
                     /locus_tag="L3M60_000113"
     CDS             complement(27400..27813)
                     /locus_tag="L3M60_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6224823.1"
                     /translation="MKKIILASHGDFSKGLLNAVKMIVGDLADCVSSYGLYPGQSASD
                     FALELEKTISEDKECEYIILSDLYGASVCTAMLRLTNLHNVKLFSGMNLNMVLELLTR
                     FSDELTVEDIDQLVEESRRGIQSVTLQIKEEEEVF"
     gene            complement(27838..28590)
                     /locus_tag="L3M60_000114"
     CDS             complement(27838..28590)
                     /locus_tag="L3M60_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6224824.1"
                     /translation="MDEKRQKKEMDLEVNSFVPLYQQLYDNIKKQIASGIYKPGDKLP
                     SEGDLCKEFNISRITVRNALNELVKEDILCKKRGKGTYVTIPERIEATCAGNSFTNSC
                     HRINAKPSTKIISVLIKKADKQVAEALSIELEEKVICIKRLRLIDEVPVIFEVDYFRI
                     DYMFLLKEELEDKSLMEVISNNISTLPKRVENIFEVKHSNKEYSDHLKCASNMPLLKV
                     RQSVYTENNDVLYYNEQFIRSDKYKYAVSAEI"
     gene            complement(28830..30224)
                     /locus_tag="L3M60_000115"
     CDS             complement(28830..30224)
                     /locus_tag="L3M60_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBN6224825.1"
                     /translation="MNLKYEFKNAWDFERKENTIEQIMQYSRNYMEFLSKSKTERLSV
                     KEIIKLAKENGYISIDEAMEKGSISCGDKIYVINKEKAVALFVIGKNYIEKGMKIIGS
                     HIDSPRLDLKPNPLYQESNLGFFKTHYYGGIKKYQWTAVPLALHGIVILNDGTKVEIS
                     IGEEDNDPVFCVTDLLIHLAGDQMQKKLSEGISGEALNILIGNIPLDDEEKEPITANI
                     LKILNEKYNIVEEDLLSAEIEVVPAGKARDLGLDRSMVLGYGHDDRVCSYAAVKAILE
                     TEQPEFTSVALCVDKEEIGSKGNTGMHSKFFENTVAELIALEGDYCDIKVRRALANSK
                     VLSADVSAGYDPNFGEAYEKRNSAYMGNGVVLTKYTGSRGKSGCNDANAEFMSEVKRI
                     FNKGNVVWQTAELGKVDQGGGGTIAHILANQGAEVIDCGVGVLNMHAPHEIVSKVDIY
                     EMYKGYKAFFNINL"
     gene            complement(30244..30453)
                     /locus_tag="L3M60_000116"
     CDS             complement(30244..30453)
                     /locus_tag="L3M60_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HBN6224826.1"
                     /translation="MFDKKKLDRINELAKKNKEGILSADEIKEREILRKEYLENFRAH
                     FRSRLDSVKVVSPEEYEQYMKNNKN"
     gene            complement(30481..31926)
                     /locus_tag="L3M60_000117"
     CDS             complement(30481..31926)
                     /locus_tag="L3M60_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN6224827.1"
                     /translation="MEKYRIDLNEKKSTKYIQIFNHIKELIINKELVEHEKLPPIRKL
                     SNFLQVNNTTIVKVYELLENEGYVYKIIGSGTFVSSLNSYKSGIDNRKNKIIVQREDL
                     IHFDNGNPSNDMFPIDSFKNAVNMALSKDGSSIFEYDEGLGSEELREKMVEYLSDGNI
                     KTTKENIQIISGAQQGIDIVCKGLISYSDVVFMEEPSYNGAIEVFKSRGAKIISIPML
                     DDGIDIGILKLKLEKIKPRLIYIMPNFQNPTGISYSTYKKKKLIELAEEHDFYIIEDD
                     FISDFVFESMDNRTIRSYDDKNRVVYIKSFSKILMPGLRIGIVEMPNELLKKVLWAKY
                     SSDISTPGLIQKSMYYYMNNFDWKNYLSHIEKIYTDKYKLAKNLINEKLSGKLKVRKS
                     CGGINFFLELPRGYTSQAFTNFMLNKGVSMLPGTYFFDNLVDDRFFRINIARPSMEEL
                     EKGISIISDNIDEFFCEYKNKLDIKSNKLFY"
     gene            32036..32830
                     /locus_tag="L3M60_000118"
     CDS             32036..32830
                     /locus_tag="L3M60_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBN6224828.1"
                     /translation="MFYDYHMHSSFSTDGKSTMEEMVKKSIELGLEEICFTDHVDYDV
                     YADDSFSIVYEDYFKSLETLQNKYKDKISIKKGIEFGVQTQLIDTYKKEAHQYPLDFI
                     ICSIHAIDTMDLYLGNYFKDKTQHEVYENYYLYLYNIVKNYKDYSVLGHLDLIKRYAP
                     YDTILDDRLFSDIIEETLRQAIYDGKGIEINTSCYRYNLPDLTPSKYILQMYKDLGGE
                     IITTGSDSHHISQVACEFDYIYSLLKNMGFKYVSKFNKLKPEFIKL"
     gene            32880..34202
                     /locus_tag="L3M60_000119"
     CDS             32880..34202
                     /locus_tag="L3M60_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBN6224829.1"
                     /translation="MKVPYLVLSTVILFISNFIVRIFGFLYKIFLSRALGETGLGIYH
                     MIFNFLMICLAVTTTGIPTALSCLVAKRKALNDRHNTNALFISTLYISFFVALTISIV
                     ASFNSSFLSIKFLKDAKLNLFILAVCPAIVIITISNVLRGYYYGIKNVKIPAIGQIIE
                     QIGKILFVFLLVMYINNKSMNCYIALLGISIGELSNIIFMLICLWRDSSFDNRYIISI
                     KDFYNSSMETLKMSIPITCNRMSNILLQSISSMMIPSRLALSGMTYQQSLSMYGVISG
                     MVMPFIFLPFTVGSALIVNLIPTISQEMALRKRKSVIKKIKYSILLTLFVGILSSIFF
                     YFFGKDLCILVFKNTLAGEYLKAMFLVPLFMSLNQTLSGILHSIRKELASSINTITGM
                     LIQLIALYVFLPIPGLNIYAYIYTMTIVSIFTCLLHTIVLFKSLKAIH"
     gene            complement(34302..35534)
                     /gene="cdeC"
                     /locus_tag="L3M60_000120"
     CDS             complement(34302..35534)
                     /gene="cdeC"
                     /locus_tag="L3M60_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosporium morphogenetic protein CdeC"
                     /protein_id="HBN6224830.1"
                     /translation="MQDYKKNKRRMTNQPMPTMNEEEVYTEEINSEDMRGFKKSHHHN
                     ECNTDNKCDCHDDCKPCNPCKPNPCNPCKPNPCNPCKPNPCDDNCGCHDHCKCDCEPC
                     EMDSDECFENKCGPECCNPISPRNFSVSNAVPFAIEANRIFDTMQFQTFTDATGPNGE
                     PLTFETEVVEVFGSVPSAGKASVTIEKICLSNDGIVIDTGMTTLEDFDLDPLGDIVGR
                     NCETTFEFAVCGERNAECCRQGKGKSVAYKQRGLTVAVRNLVLELRGRCGCTEFVALA
                     FPAVRAGGGCKRRVDYVEFTFNTLSAPICLPADGRAVTLRQEYQTNLTVDCIGKSILK
                     LECNECCEPFYELIIPNDIDLVLCLQNTVSTLISEQIVVLASPNPIQPRLVDTFSKVC
                     DFSQCGPNHESGKPSCHR"
     gene            complement(35719..35899)
                     /gene="ssrS"
                     /locus_tag="L3M60_000121"
     ncRNA           complement(35719..35899)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="L3M60_000121"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(36014..36925)
                     /locus_tag="L3M60_000122"
     CDS             complement(36014..36925)
                     /locus_tag="L3M60_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224831.1"
                     /translation="MNNLLFLIGVIALSNGNISIFDDKNKSKGNNRVRKNKKNANDKL
                     SQSTHSNLRFDANDIKRGLRLMDLSEEDLEMGMEIITRTKKYMSKDERKILIKIESVL
                     DLVRGIKKLNNIDMVDIEDETDFFRKMDSDDKKNMMIKEIIDVFPEKRKNSVEKAIGM
                     KKKIDLFAELFLPDDFGEGGFSLSSLANINNLGSMNNLKLLGSLLRGDDSNNEDEDNN
                     EGLNNEDEELDYEDKELDYDDEELTDEDEMEDNEDELVEDNKTDYENIENDEDIVYDE
                     ELYNINEQESEYNYNAKNTKTRTKKKR"
     gene            37137..37535
                     /locus_tag="L3M60_000123"
     CDS             37137..37535
                     /locus_tag="L3M60_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224832.1"
                     /translation="MKKLDAESLEKMAKQKNIDKDKIEKMADSYKGKSENELMEELIK
                     IGKNLDGRDEVVSKFKAFLDENQRKKLDTIMEKISDAETQRDTKPAKTKKTKPTKGKS
                     NSPAPKNTPTGQQKKSKGLFKKTKKSNPHE"
     gene            complement(37657..38673)
                     /gene="ccpA"
                     /locus_tag="L3M60_000124"
     CDS             complement(37657..38673)
                     /gene="ccpA"
                     /locus_tag="L3M60_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator CcpA"
                     /protein_id="HBN6224833.1"
                     /translation="MKGNITIKDVAKQAGVSISTVSRVINDSKPVTDEVKQKVLEVIK
                     ETGYIPNPLARSLVTKKSQLIGVIVPEVSDSFVNEVLNGIEEVAKMYDYDILLANTYS
                     DKEQELKSINLLRAKQVEGIVMISWIVEQEHINYIQNCGIPATYISKTARNYDIYTVS
                     TSNEEATFDMTEHLIKKGHEKIAFIMTSKDDTVLEMERLAGYEKALSNNNIELDKSLI
                     KYGGTDYESGYNSMKELLDDGIIPHAAFVTGDEAAIGAINAICDAGYKVPEDISVSGF
                     NDVKIAKMYRPKLTTVYQPLYDMGAVAIRMVIKLINKELIENKKIELPYRIVDRESVI
                     ERKK"
     gene            complement(38926..39126)
                     /locus_tag="L3M60_000125"
     CDS             complement(38926..39126)
                     /locus_tag="L3M60_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224834.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFEDCWRDIDIMSWIPF
                     LILLLITCSSGGFFDGEVGCGC"
     gene            complement(39160..39339)
                     /locus_tag="L3M60_000126"
     CDS             complement(39160..39339)
                     /locus_tag="L3M60_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224835.1"
                     /translation="MLDRFFGEEGCFGGGGWWIIVLFFLFLAFGDSWAEFDIMAWIPF
                     LIVLLILCCCGGCLE"
     gene            complement(39521..39727)
                     /locus_tag="L3M60_000127"
     CDS             complement(39521..39727)
                     /locus_tag="L3M60_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224836.1"
                     /translation="MTKLEDNMERFFGGCEGFFGNKMLIIIVIVFLLLCTDILEDLLC
                     DDNIWIWVILIVLLLFNFDDGCCC"
     gene            complement(39775..40200)
                     /locus_tag="L3M60_000128"
     CDS             complement(39775..40200)
                     /locus_tag="L3M60_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224837.1"
                     /translation="MKYVGLLLTSIGMFLLIAVNFYYNSITLDMQRIEDYVMETNLIL
                     EDVAEKESYVSNEKEDYISRLMHVKKGIENSKTSFLIERYKEYKIKSIESLIYTISEE
                     KKDYLDEVDRYNKLGEKEINKLINKNFLEVTYLSITTYI"
     gene            40372..40599
                     /gene="acpP"
                     /locus_tag="L3M60_000129"
     CDS             40372..40599
                     /gene="acpP"
                     /locus_tag="L3M60_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBN6224838.1"
                     /translation="MFEKIKEIIAEQLGVDNLDEITMEASLMDDLEADSLDAVEIIMA
                     LEDEFGIEIPDEEAENFKCIGDICKYIEENK"
     gene            complement(40740..41744)
                     /locus_tag="L3M60_000130"
     CDS             complement(40740..41744)
                     /locus_tag="L3M60_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HBN6224839.1"
                     /translation="MIIHKFIIHVLDKNSDVPILNDFEGKVNQEVDGFFQKAIKRIAK
                     DEDLRKGVFKDYNDNLIKNCCEQIIYDESTFLKNSKEIASYLFDVMKINAALESCDLA
                     ICLYTIKDEKSVAILKLDYKKLYTHSIEFVDDKFNIQMVPNEIGIPETLRQKQGALIS
                     LSGINDEFHLRLLDKDAEKEGSESKFVTEFLNAKKIDDDKYKTKVFKNTAENWITNAL
                     SNDIKQAEDVRSILNYTLKEKHEVDINDFVDNSIKDDELKDSFKEHMEEKGLDESFSI
                     DKKWVEKKLKKRSIKTDNGFDIKGNLTDFEDPMKYTVKQNQDGTIDIIIKNVTFYEEK
                     "
     gene            complement(41758..42660)
                     /locus_tag="L3M60_000131"
     CDS             complement(41758..42660)
                     /locus_tag="L3M60_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBN6224840.1"
                     /translation="MFKKENQRYNLISYTNEEEMTLKEVLLDKLNFSVRSLSKMKREK
                     SVLVNGVYKKPSLKVYSGDLIEVKINEEKANFEPQDLNLQIIYDDFDIIMVNKLPFMV
                     VHPTKSHYDKTIANGISYYIDNQKENVKIRFVNRLDMNTSGLVIVAKNAYAHHTLSTA
                     MSENKVEKKYITVVDGIIKENEGTIDEPIYRPTEDSIKRIIDERGQSSVTHYKVIERL
                     ENATVLEVSLETGRTHQIRVHMAHIGHGIIGDELYGCVDEELINRQALHAYRLEFEQP
                     RTKEKLKFKADIPEDIKELISKLR"
     gene            complement(42857..44032)
                     /locus_tag="L3M60_000132"
     CDS             complement(42857..44032)
                     /locus_tag="L3M60_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBN6224841.1"
                     /translation="MREVVIASAARTAVGSFGGAFKSVSAVELGVTAAKEAIKRANIT
                     PDMIDESLLGGVLTAGLGQNIARQIALGAGIPVEKPAMTINIVCGSGLRSVSMASQLI
                     ALGDADIMLVGGAENMSMSPYLVPSARYGARMGDAAFVDSMIKDGLSDIFNNYHMGIT
                     AENIAEQWNITREEQDELALASQNKAEKAQAEGKFDEEIVPVVIKGRKGDTVVDKDEY
                     IKPGTTMEKLAKLRPAFKKDGTVTAGNASGINDGAAMLVVMAKEKAEELGIEPLATIV
                     SYGTAGVDPKIMGYGPVPATKKALEAANMTIEDIDLVEANEAFAAQSVAVIRDLNIDM
                     NKVNVNGGAIAIGHPIGCSGARILTTLLYEMKRRDAKTGLATLCIGGGMGTSLIVKR"
     gene            complement(44120..44965)
                     /locus_tag="L3M60_000133"
     CDS             complement(44120..44965)
                     /locus_tag="L3M60_000133"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HBN6224842.1"
                     /translation="MKLAVIGSGTMGSGIVQTFASCGHDVCLKSRTQGAIDKCLALLD
                     KNLTKLVTKGKMDEATKAEILSHVSSTTNYEDLKDMDLIIEASVEDMNIKKDVFKLLD
                     ELCKEDTILATNTSSLSITEIASSTKRPDKVIGMHFFNPVPMMKLVEVISGQLTSKVT
                     FDTVFELSKSINKVPVDVSESPGFVVNRILIPMINEAVGIYADGVASKEEIDEAMKLG
                     ANHPMGPLALGDLIGLDVVLAIMNVLYTEFGDTKYRPHPLLAKMVRANQLGRKTKIGF
                     YDYNK"
     gene            complement(45063..45860)
                     /locus_tag="L3M60_000134"
     CDS             complement(45063..45860)
                     /locus_tag="L3M60_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBN6224843.1"
                     /translation="MSTSDVKVYENVAVEVNGNICTVKMNRPKALNAINSKTLEELYE
                     VFVDINNDETIDVVILTGEGKAFVAGADIAYMKDLDAVAAKDFSILGAKAFGEIENSK
                     KVVIAAVNGFALGGGCELAMACDIRIASAKAKFGQPEVTLGITPGYGGTQRLTRLVGM
                     AKAKELIFTGQVIKADEAEKIGLVNRVVEPEILIEEVEKLAKIIAKNAQLAVRYSKEA
                     IQLGAQTDINTGIDIESNLFGLCFSTKDQKEGMSAFVEKREANFIKG"
     gene            complement(45879..46889)
                     /locus_tag="L3M60_000135"
     CDS             complement(45879..46889)
                     /locus_tag="L3M60_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBN6224844.1"
                     /translation="MGNVLVVIEQRENVIQTVSLELLGKATEIAKDYDTKVSALLLGS
                     KVEGLIDTLAHYGADEVIVVDDEALAVYTTEPYTKAAYEAIKAADPIVVLFGATSIGR
                     DLAPRVSARIHTGLTADCTGLAVAEDTKLLLMTRPAFGGNIMATIVCKDFRPQMSTVR
                     PGVMKKNEPDETKEAVINRFKVEFNDADKLVQVVEVIKEAKKQVKIEDAKILVSAGRG
                     MGGKENLDILYELAEIIGGEVSGSRATIDAGWLDKARQVGQTGKTVRPDLYIACGISG
                     AIQHIAGMEDAEFIVAINKNPEAPIFKYADVGIVGDVHKVLPELISQLSVAKEKGEVL
                     AN"
     gene            complement(46908..47690)
                     /locus_tag="L3M60_000136"
     CDS             complement(46908..47690)
                     /locus_tag="L3M60_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBN6224845.1"
                     /translation="MNIVVCIKQVPDTTEVKLDPNTGTLIRDGVPSIINPDDKAGLEE
                     AIKLKEEMGAHVTVITMGPPQADMALKEALAMGADRGILLTDRAFAGADTWATSSALA
                     GALKNIDFDIIIAGRQAIDGDTAQVGPQIAEHLNLPSITYAEEIKTEGEYVLVKRQFE
                     DCCHDLKVKMPCLITTLKDMNTPRYMKVGRIYDAFENDVVETWTVKDIEVDPSNLGLK
                     GSPTSVFKSFTKSVKPAGTIYNEDAKTSAGIIIDKLKEKYII"
     gene            complement(47749..48885)
                     /locus_tag="L3M60_000137"
     CDS             complement(47749..48885)
                     /locus_tag="L3M60_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HBN6224846.1"
                     /translation="MDLNSKKYQMLKELYVSFAENEVKPLATELDEEERFPYETVEKM
                     AKAGMMGIPYPKEYGGEGGDTVGYIMAVEELSRVCGTTGVILSAHTSLGSWPIYQYGN
                     EEQKQKFLRPLASGEKLGAFGLTEPNAGTDASGQQTTAVLDGDEYILNGSKIFITNAI
                     AGDIYVVMAMTDKSKGNKGISAFIVEKGTPGFSFGVKEKKMGIRGSATSELIFEDCRI
                     PKENLLGKEGQGFKIAMSTLDGGRIGIAAQALGLAQGALDETVKYVKERVQFGRPLSK
                     FQNTQFQLADMEVKVQAARHLVYQAAINKDLGKPYGVEAAMAKLFAAETAMEVTTKAV
                     QLHGGYGYTRDYPVERMMRDAKITEIYEGTSEVQRMVISGKLLK"
     gene            complement(49593..50060)
                     /locus_tag="L3M60_000138"
     CDS             complement(49593..50060)
                     /locus_tag="L3M60_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HBN6224847.1"
                     /translation="MSISNVREYFKQFGKDDSILEFEQSSATVELAAEAAGVIPARIA
                     KTLSFKIGDDAILIVTAGDAKIDNKKYKAEFNCKAKMLIPEEVLEFTGHAIGGVCPFG
                     LKNSLKVYLDDSMKRFDTVFPACGSSNSAIELTCEEMEKFSKCEKWVDVCKNW"
     gene            complement(50139..50681)
                     /locus_tag="L3M60_000139"
     CDS             complement(50139..50681)
                     /locus_tag="L3M60_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224848.1"
                     /translation="MKKFIKVSFFIFGIFTLFIAINKLVYRDMVSVKDYEINHLEEIV
                     FDFDSRRSRDIGTIVRFKISNNSKYSYKLNSAKMKFENCVENKKGINKCSSYVNIDLF
                     NEKNSITERTLKYGIKPNNQGYVDFVIPKGLNFDYRYFNESGMSVEYKGEYVVNIPMA
                     KGLYLVVGKNNDTWNANIME"
     gene            complement(50733..51419)
                     /locus_tag="L3M60_000140"
     CDS             complement(50733..51419)
                     /locus_tag="L3M60_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBN6224849.1"
                     /translation="MFNLIRKDLIIGISSDGIRNLKYILLFFVFYFFLNSISYYTVSI
                     VVSYLIFINTFECDYENDSRIFIRSMPVSIEDIVYSKYLLGVGLIISVTIIVSLMSKL
                     TSLVFFRNMVLNDVFFSVNIFLAILTILLPLIFKFGYGKMKVCGLIVSILIYFVYGSL
                     LKMISMIVYQVKHFNYSKVGGVYLSNYVTDMANAKYINLYSMTFLTIIIFIISMYFSI
                     RISKKNKFNY"
     gene            complement(51419..52279)
                     /locus_tag="L3M60_000141"
     CDS             complement(51419..52279)
                     /locus_tag="L3M60_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6224850.1"
                     /translation="MFSIRNITKKLGKFKLNNINLELKEGDIVGIIGPNGSGKTTLIK
                     IIMGIIDADEGEIELCNETIENSPISFKNNIGFVYDSLQFYPHLKVKEFRKIVSLFYK
                     EFDRERFDEYLNKFDIEENMHIKNLSKGQSEKLMLSSALSHNAKLLILDEPTTGIDPI
                     VRSEIMQYLQDFVKNGSSSVIISTHNTDNLIKIADYLVFINRGNQIFTVKKELIEQEY
                     KIIRANKAELEAIKESIVGVKEYKYYNEALVKVGDSLKVKSLLIEIDKHKVKNPTIEE
                     LMYYYVNEVR"
     gene            52413..53213
                     /locus_tag="L3M60_000142"
     CDS             52413..53213
                     /locus_tag="L3M60_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HBN6224851.1"
                     /translation="MKRIITINTNQLNKSLETKELLTRKLINAGFEVYSDIYPDTELI
                     ISIGGDGSFLRTVRDFDFPEIPIMGINTGHLGFFPDILPDKIDSFIEAYTKKDYIIQE
                     MSLLNAEVYTTTSGSNMLAVNEVVIRGDKSRTIHLNLSLDNKHIQNFSGDGMIISTST
                     GSTAYNYSAGGSIVDINLELMQITPLHPINTNAYRCFTSSIICSNESVIKIAPEYRFE
                     DSVLIVVDGVEHRFRQIENIKVSISDAKIKLLRMSNYEFWHRVSEKFL"
     gene            53238..53807
                     /gene="yfcE"
                     /locus_tag="L3M60_000143"
     CDS             53238..53807
                     /gene="yfcE"
                     /locus_tag="L3M60_000143"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBN6224852.1"
                     /translation="MKIGIMSDTHGSLLYFEKALNVLSDCDVLLHGGDVLYHGPRNDI
                     PEGYNPKKFIETLNKLENIVIVKGNCDADVDQMVIEHPIQSPYVMSQFGEIRIILNHG
                     YTESEEEIIDKAKKMGGDILVLGHTHVKKLYMDDNLIVINPGSTSIPKDGSHSVAIID
                     IIKTDDEDELELDINLIDINTGNIININD"
     gene            complement(54065..55117)
                     /locus_tag="L3M60_000144"
     CDS             complement(54065..55117)
                     /locus_tag="L3M60_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp18"
                     /protein_id="HBN6224853.1"
                     /translation="MKIKSRKIIAFVLSLGIMMPSCVYADTISKNQNTEVDMNNYLQG
                     RKSPKEETLIGEDRFDTAIKISQSGWNNGSERVFLVNSNSLPDALASTPLASKLDAPI
                     LLTNKNSIPYNVTEEIKRLNPAEVILIGSEGAISSSVKEFLEDMGVLVSRIGGADREE
                     TSLLLTKQLDDTGDLGVSKVAVVNGYNGLADATSISSPAASDNTAIIYTGKDSIRSEA
                     KNFITQNSTETYIVGGEYSISKKLEGQLVNAERLAGTDRKDTNAKVLEKFYGKSSKVN
                     NMYFAKDGSGREADLVDGLAAGVLASKTKSPVVLASGSLSSAQKSFIKKVKADKFVQV
                     GGGKNSKPYAEALALQ"
     gene            complement(55324..56550)
                     /gene="pepT"
                     /locus_tag="L3M60_000145"
     CDS             complement(55324..56550)
                     /gene="pepT"
                     /locus_tag="L3M60_000145"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBN6224854.1"
                     /translation="MKQKVVERFLKYVSFDTTSNSQCENCPSSEGQRVLAKYIVEELK
                     TMGVDDVSLDENSYIMATLKGNTDGVDTIGFISHLDTIEDVSGKDIKPRIIENYDGKD
                     IVLNEELNVITYVKDSPELVEFKGDDLIVTDGTTLLGSDDKAGIAEIVTAIEYLINHP
                     EIKHGDIKIGFTPDEEIGRGADLFNVEKFGAKYAYTLDGGIVGELECENFNAANATIT
                     IHGRNVHPGSAKNKMVNAIHIATEISEMFPADERPETTEGYEGFWHLNSIDGNVENVS
                     MSYIIRDHCREKFEYRKSIMVENIEQINKKYDNRVELDLKDSYYNMKEKIEPVMFIVD
                     IAKEAMEELNIKPRLVPVRGGTDGARLSFNGLPCPNIFTGGLNFHGKNECIPVSSMEK
                     ATKLIVRIAEKYAERV"
     gene            complement(56643..57695)
                     /gene="ytvI"
                     /locus_tag="L3M60_000146"
     CDS             complement(56643..57695)
                     /gene="ytvI"
                     /locus_tag="L3M60_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBN6224855.1"
                     /translation="MSILENNFLYKLKNNTIFFCIYTLLFIFIYKAFPYIAPFFLGGI
                     IALMINPISQKLENKFHINKGISTLVLSFLAVAIVSTVTTIIVINSVKELMGFLNNIS
                     ANPEDISNTIMYFLNKINDFMKSFQEIANFDIEQLVNKFSGEVMQITKNLLTSILGLA
                     TSIPYIIIFIITLFIATYFIAKDLDKIENSFYNMFTVDVRKKVKNVKKEAGLSLVGYI
                     RAYTILMAITFFAIWGSFALFGLKYGLIVGFVGALMDLIPFLGIITIYLPVIVYYFLI
                     KNYFIAISMTVIFFVLSLIREILEPKLVSVNVGLNPLATLAAIFIGIQVKGIIGVIFC
                     LGLVCMHDILKKVDIF"
     gene            complement(57843..59096)
                     /locus_tag="L3M60_000147"
     CDS             complement(57843..59096)
                     /locus_tag="L3M60_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBN6224856.1"
                     /translation="MLESPDNLIQIIFLIVLLIGSAFFSASETALMSLSKIRIRYMQD
                     EGVKGAKLVSSLIENPNKLLSSILVGNNVVNIAATSISTSLFIGLMGEKGVALATAVM
                     TVLVLIFGEITPKTIAANNSEKVSLLVSKPIKAIIFILRPIVWIFNIITNIIFKLFGI
                     TNKGAKSFITEEELKTMVNVSHEEGVLEMEEREIINNVFEFGDMQAKNAMVQRIDMVA
                     IDMEDSYDEIIQVFKTEKLSRMPVYEETIDDIVGILNIKDIIFLSDEEIESFDIKNYM
                     REPFFTYEFKKITQLLEEMKLEKSQMAIVVDEYGGTSGLLTIEDLVEVIVGDIEDEYD
                     EEEDEIQVIKEDEYIVDGSTKIGDVNELIGVNLESEEFDSIGGFIIGHLSRLPEENEV
                     IEVDNIRFCIESIEKNRIKKIRIYT"
     gene            complement(59227..62754)
                     /locus_tag="L3M60_000148"
     CDS             complement(59227..62754)
                     /locus_tag="L3M60_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6224857.1"
                     /translation="MRPIRLELTGLNSYIDKQVIDFEKLIERGLFGIFGTTGSGKSTI
                     LDAITIAMYGNISRNTKEYINSVCDKAIISYEFEIGSKNTRRRYIVDRTIARSKTGTK
                     TSNARLIEVLNDNTQNVLADKVVEVNEKVAQVVGLTANDFTRSVVLPQDKFNEFLRLS
                     RADRRDMLERIFNLEKYGRSLGEKVKKRKNLQLQSLKDLKSKLSQYDGITEEVYNNIN
                     QELIELKNLEKDKNNALDLAQNSYEESKTVYEEQLKLEKNELRKNELDLKNGEIKEKI
                     ALVENDSNAKKVDPYIGSVQNLEKKIDEDSFTVSNLEKKLVILNQELEVIKNRYEKIN
                     KIKNEEVPKLSEEKIRLQQAIKLEEELVLLDKELKELKESGINLNKTKVELEKVKQMS
                     ESRKDAVTKSIKEVEGKINKVNISAELKQKIFLAYEYEKDYNNVLEEKNQKINKLEEI
                     LKETEDINLKVRYIDKDKNDVNRNLENLSLHLDVLLKKCPGKSADLLLKSEYVTELRN
                     KADNTKENEIKKSSIQDELKIILESKFNTEREVNLLNEKLENNRKNRDDLEKELEQLK
                     YLNLASELRRELKENMPCPVCGSKHHENHNITKYDENISFVKEKLEKLEKEKISIRNN
                     IEELNAKLSGYLSIEKMKTKELEDVKGKLGDIPSSQLLKKLDEEQRKLALLKSNIQEW
                     EKEKESTENKVTLAKEEKNKIEKEELKIRESLNNYKKLTRDLNIEIEGLENKCKKLKQ
                     EYIGLKTITKVSNLLSKVKEIRENEKELELLSIGHSNLLKNRDSLDIKIRENESKLHE
                     IEIELIKARELYLEKKLSRDNKYKEVINITKGDLAKNLLHSVEEDICKILEQEESSKK
                     KLEQQRLEYEKNIAEKHNIDGRLKTAKEQYKDQKSTLNKLLAENKFESIYAVKRALLD
                     ADEVKKLIEEILEHEEEQKLLAFKIKSSKEKLNGRSVKKESFEQLKDEIYNLKVEIGN
                     ISKNIGANQNQLITLKDSLDKINDFNKQLKVVEHNVDLLEELDKCVQGNKFVEYVSTN
                     QLKYIALEASKRLDGITKGRYALEIDSTLNFVMRDNFNGGERRSIDTLSGGETFLTSL
                     SLALALSSQIQLKGSAPLEFFFLDEGFGSLDSELLEIVIESLERLHSNNLSVGIISHV
                     EELKNRVPVKLLVSSSEAGIGSKVKIEYS"
     gene            complement(62741..63952)
                     /gene="sbcD"
                     /locus_tag="L3M60_000149"
     CDS             complement(62741..63952)
                     /gene="sbcD"
                     /locus_tag="L3M60_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBN6224858.1"
                     /translation="MRFIHTSDWHLGKSLEGHSRIEEQAKFCEEFIKIVESNEIDMVI
                     IAGDVYDTSNPPAQAEKLFYQTVSRLANNGQRCVLIISGNHDNPERLSAITPLAHEQG
                     ILIYGYPLSATIEAKYKGFEITCATQGCTKLNINGENVVVATLPYPSEKRLNEVFSSE
                     DDFEKQKNYSEKVGDIFRSLEENFRSDTINIAVSHIFVIGGESTESERPIQLGGSFLV
                     ERKDLPEKAQYTALGHLHKQQKASERLNAYYSGSPLQYSKDERAYTKGAYIVDIKAGE
                     KPIIEDVYFNNYKPIEVFKCNGIEEALDICEQNQDKEIWSYFEINTEEVISQNEIKKM
                     KELLKDIIEIKPIITSCYEQESVDIKEKSMAELFREFYSFSKGVEPKGELMDLFLDII
                     SEEGESADETN"
     gene            complement(63996..67823)
                     /gene="addA"
                     /locus_tag="L3M60_000150"
     CDS             complement(63996..67823)
                     /gene="addA"
                     /locus_tag="L3M60_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HBN6224859.1"
                     /translation="MSSPKWTKEQLEVIESRECNLLVAAAAGSGKTAVLVERIIQMIT
                     SRENPIDIDKLLVVTFTNAAASEMRERIGDAIGKALDENPENKHLQNQLVLLNKSSIT
                     TIHSFCLDVIKSNFHRISLDPNFRIGDQTECAILKQEAIEEVFEDLYEERDEGFLNLV
                     ESYAERGGDKEVQDIILGIYSFAMASPEPKKWLIDSAERFNIDENFDFSQSIWARAIL
                     DTVKIEINGLCLNMERALKEVESIEELETFAEKLSAEYKKIVDISQACNKSWDETYKK
                     MSSMSFENYVKGVKRISKDAPSYIKESKEKAKTIRDKTKKSLESIVSATFNKDNDSIR
                     EEIKYLYNIVKPISNIVLRFEEEYSNKKREKGIIDFNDIEHFALNILTDVDEKGNIVP
                     SDIAVGYRNEFYEIFIDEYQDSNLVQEVLLKAVANTETPNRFMVGDVKQSIYRFRQAK
                     PELFLQKYNNYNDKKGSSHRKIMLYKNFRSREEVVDAVNYIFENIMNENIGEIEYTEK
                     ERLNLGANFEMDTDEKSIIGGATEIHLIQKDNKLDDDIINDKDDRTNNKESEIEEEEK
                     LDNIQLEARMVGKIIKDLMKVNEEGKLQKVYDKGIGGYRPVEFRDIVILLRATSAWAP
                     VFADELMNMDIPTYADVGVGYFDTIEIKTILSLLQIIDNPMQDIPLISVLKSPIFGFT
                     PEDLIDIRVQSKDKIFYEVLKSTAEYNEFKDSQNENESEFIPSEECINKSKDFLIKLK
                     EFKEKSMYMSTDEFIWYLYTRTGYYAYVGALPGGSQRQANLKVLFERAKQFEETSLKG
                     IFNFVNFIEKLKKSSSDMGSAKTLGENANVVRIMSIHKSKGLEFPVVICSAMGKNFNT
                     QDFKKSILYHHNLGYGPQFVDYERRISFPSIAKEALKSKINIENLSEEMRVLYVAFTR
                     AKEKLIITGSTRNIQDSIKRWSNGVENSDTISQYEILKGKNFLDWIMPCVLKHRDLSN
                     LLDEVGLDAVFSVEHNSKWYGKLWDKNDILVEKKNNEEKESIEKILEKIDVDSPDSDY
                     YNEIEEKLNYIYPYEFSTRKPATISVTEIKKIQNNYEEELINTIFEQKVILKKPLFIQ
                     NEEERDKISGAERGTIVHLVMEVLDLKNVSNVNDIKSQIRGFVSKGIITEKQASIVNP
                     YKIYKFFESNIGKRMLNAELINREKSIYAQVNMKDIYIYEKLINNDDKKLYDEESVML
                     RGIVDAYFEEDNQIVLVDYKTDFVNEENINQIIEKYKKQLDLYADIIETLTGKSVKEK
                     CIYLFGVDEAVCY"
     gene            complement(67823..71290)
                     /gene="addB"
                     /locus_tag="L3M60_000151"
     CDS             complement(67823..71290)
                     /gene="addB"
                     /locus_tag="L3M60_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HBN6224860.1"
                     /translation="MGLRFVLGRSGSGKSTYILDEIKKEAQKNETTSIILLVPEQYTF
                     EAENRVSKLFLGKEKDKYLRVRMLSFKTLSNIVFSQVGGLTDVNINSSGKAMMVYRAI
                     EDVSEELNVFSKSKSQAGFVSSITEMISEMKQYNISPEMLENISGELDNETLSLKLKD
                     ISKIYNSFEGKLHENYVDAQDMLTSLASKIELSSYLDGACVYIDEFTGFTPNQYNVIK
                     SILNKSKSVNISLTVDDVNYTGYSKADMFSRTKFTYSKLTQLCNEEGIKILPQVNLNT
                     GVIKRFEKVKELQHLERFYNAYPYKIYSKQTENIKIKEFNNLYSEVEEIAREIVHLVR
                     EKNVRYRDITIATRDLNRYDFLVHSIFNEYNIPNFIDKKREAKSNPIVILIISALEMK
                     NRRYGYETMFRYLKSGLIGIDNDDINVLENYVLANGIKGKKWFDEKWDYRITQSLSGE
                     ESEFELELKDKINEIKNRVLEPIVTLQEKLKGKNRVKEICRYIYEFLLDINMPETIES
                     LIVNFKDKGELDVANQYSQVWDIVVDILDQMVELMGDEIISLEKFIKLITLGFDEYEL
                     GLVPPSIDQVLVSSVDRMKNPDTKYLYLVGTTDGVFPLITKDSGILSDNDRESLGNKG
                     IEVDIDSQTRSFEEQFLVYKALTSTSKNLTITYPISDHEGKTLRPSIIISRLKKIFPN
                     IENKSYLVEENKSTDEDILKKITVKSPTFNELINVIKNYDSDGYNKEEINSIWLDIYR
                     YYLKDEIYSSITKKVIKGLSYTNQVHRIEEKKIRSLYKSNSLSISRLEKYAECPFAYF
                     IQYGLKARKRKEYSFTPPDLGTFIHNILDRFSKELLQDNLSWRGIDEKYIELKIGIIV
                     DEIILKIPGYILNSSERYKYLAYRLKNMLTTAITIISQQIKQGSFEPIDYEVKFGDNG
                     KYPPIKIVLENGQEVSLIGQIDRVDEFEEGENKYIRIVDYKSGNKSISLTEIYYGLQL
                     QLLVYLDAILESAKDKDININPAAILYCRINNPIAKFNEDKDDVEIQEAILKELRMKG
                     LVVKDSHIVKKMDKSLIDGERKNSLVIPVGLTKDGNVGKSTSAISYEDFKLLRKYVKH
                     AIKDLCEDMLSGEICIAPYKHKDGTSCDFCDYSAICQFDSTMKDNKYKNLNNKSNEEI
                     IKMMKGDVN"
     gene            complement(71280..72110)
                     /locus_tag="L3M60_000152"
     CDS             complement(71280..72110)
                     /locus_tag="L3M60_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HBN6224861.1"
                     /translation="MNDKLKYFSYSQNSINTYKSCPLKFKYKYIDRINWKNDDVGSRE
                     YYETLKTGRDFHLICERYFSNIPLGIYFNEDNKNSKKFLKWMENIKKVVPIEKGKTYL
                     PEYEVRMSLNGDTIQAKYDLVVIGNNNIDIWDWKTESKKIDYKHVENRIQTIVYMFLA
                     KEVIPKVLKMDINVKDINMRYYQPEFDDLPITILYNEEKHETNRNKIQNYISMIKNTN
                     YEEHTYEDNLYNDIEKVYNKEERMCYRNKKHCKYCEFNKLCNGKEIDYSILEAEIYGT
                     "
     gene            complement(72219..74438)
                     /locus_tag="L3M60_000153"
     CDS             complement(72219..74438)
                     /locus_tag="L3M60_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBN6224862.1"
                     /translation="MININYREDQIPIINYDNGTMAVPAVPGAGKTFIITNLVAKLLL
                     EQKHKGGKILVLTYMNSAVNNFKGRIRKILEEHKIDDTNSYEVMTIHSLAVKIIKEKP
                     EVVMLSEDFNIADDLQKNIILNECISRFRFEGGERAFRWFLKEQKDERWKEITLEAWE
                     RGFFEFAGNAISELKYKGISPDKLEEILSRGQKGMLKIILPIYKLYDRKLKQNGLLDY
                     DDILILAEKTLSLDEGLRKKFQTRYKYIFEDECQDSNEIQGNIIKIISSENRNLVRVG
                     DINQSITGTFSSSDPKFFKEFINSADFCYRMDMSNRSSKDILDLANTLVEYVTKEFRQ
                     EECRHALEDMQIKTVPNGMGYKENPNPEHYNINIKWYDSWKFEIEQTARYVKGIKNKY
                     PDKSIGILVPFNEQVTQVAKELRENNLVFEELGPNSLSKRRVINNIASIIDFILHCDD
                     IEKLIVVLDKVFIDTDNEEELKYFLEKLKNYSTEEIIYDFEFSKLDNDLIEFNEESDI
                     YLNFKRGINIIKQILEYPIIRLDLLVLFIGKKLNLEKEDKAVLDYISFYIKYLASENI
                     NMDLEDVYNLLFDVKNKVFNHIIDVVYEINGYEPEPGSITICNYHKSKGMEWDCVFLL
                     GLVEYNFPDNINQKFQSDKWYLKEKYKNPMAIIKSEVEAILKGSISTDYAHKTKIDSI
                     NEKIRLLYVGITRAKEMLVLSGSAYRDESDIGNKRKEQKPCIYLGRLNRHIIKKRSN"
     gene            complement(74431..76344)
                     /locus_tag="L3M60_000154"
     CDS             complement(74431..76344)
                     /locus_tag="L3M60_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224863.1"
                     /translation="MEYLIDKSSLKECGNEPLDKYREILDKTKNNSRSILLLVPNNNT
                     KIQYERSLRLNYSEELKITTYISFVKKELLKYWPLIIENCDEINKKAVSPTFISNSLS
                     EYLIVQKVKEKRIQEGYFEDITGTNKSIAKSIMTNINKSAFNLIDFDFIGEKIYSSKK
                     NKDSIYRFSYTQMDEIISYYINSLLSNSILDNSLSVYLYNQYLLNDDFYVKSLFKEVR
                     YIIVDSLESSSNAEVDFITEALDNTLQSYIYFDYSRDYSVFNNVDMDYINEKIISKIK
                     SKEENIKRKEIGIEDLYLLPATIKLNQSSQLYNEMLDLISEKIISLIEEGVSLRDIAI
                     ISPINNSILEYQIRDSLIEKNIDVFNIKKDKKAVDYPYGNALVVATCMFYGYLDFIKD
                     DEYLSFLETLLEINRIKAFKIFNETRHLSIDNMDEHSQYRDILQYIEEKKKSDLKIHE
                     FLIQFYIDKMLSLKDGKKNVGLCKKIIAESESFSESLSLLGMKKEEIFIEALKTAIND
                     YYSVVDIEELKSKDKIVITTPYSYIASNIDRSIQIWVDIGSNAWNMKIEKDISNLIVL
                     RKSFEEKKIYTNEMEEYYKKYYLYNTVYNLLLNAKKIYAYKSEYAINGYIQESILYSI
                     LLKISHKGDENYD"
BASE COUNT        24390 a        13610 c         7679 g        30747 t
ORIGIN      
        1 ttatataaaa attactttca aataaagtac tgcaatattt ttatttaaag tatctataat
       61 ctttaaccac aaaaagccac aaaatacaat gtatacctat ggctttttat aaatttacat
      121 aattgtatat aaagatatac tctaaaactg tcttaagaat ctaaagttta aaaatacaat
      181 atttttattg tgaatttaaa ctttttatta caacttttta tctaatttat tgtatacaac
      241 aacactacaa ataaattgaa ataaaataag caatccaaga acaactaaaa cccacttgct
      301 tattaccatt acagaaaata cactcaatat agaaacaata aatataagaa ttacaagaac
      361 ataggacatg atttgattag tgatacgtcc agctttatca cgtaacataa ttcttctttc
      421 atcattcatc tctatccttt ggttttttat cttttcttca taatctaatg ctctttcagg
      481 cttactccaa tacatatatt gaagaatcat acatacacct ggacctaaag cagctccaga
      541 taatccccaa ataaaagcct caaatgaaaa ctcagtaaac gtagccaata aaacaagtaa
      601 aattccacaa ataaaatata ctattccaag atataacata ttctttttca tttttactcc
      661 tcctcataaa taaatatttc ttcaatactc ctatcaaagt atctagctat cttaaaggct
      721 aagatgatag aaggattata cctaccattt tctaatgaac ctatagtttg tcttgatact
      781 tgcaaaacag aagcaagttc ttcttgtttt atgcctcttt cttttcttat ttcttctaat
      841 ctatttttca catctatccc cctttaatat ctcaactacg gaaagtctac tttccataat
      901 tatacaacta tattccattt atgtaaagct tactttccat atttacaaaa aaataacaga
      961 ctattttcag actgtaattt tagaatttca tgttgtttat taattttaat gttatttatt
     1021 aattccatat tatttattaa ctttaacttg gtttctgaat tggaatccaa acttctgtag
     1081 tataatcagg agaattgata tcgccattgg gataaagttc aatatctggt aaattagaat
     1141 acacataccc agaacttggt aaccactcag taataattct tttttgcaac tcctgtattg
     1201 ccagagcatt tggcattgga gaaacacatt caaatactgc ccatgtacct gatggtatta
     1261 agtattcatg agtatcttct aatataggct tatctgttgg tgcagcaata taataatcta
     1321 agcccttact atttgtacac acactaactc caagcaatcc acaaggttcg ttatttatta
     1381 actctgaaag tttatcaatt gttccatttt ttatgcattt agcccataac ttaggaactt
     1441 tctcaaaaca ctcttctaca atcatgttca tactttcttt tataccaaca atacgaaatc
     1501 ctttcttttc tacaatacga tattgcattt ctacatttcc ttttacataa atgcttaatg
     1561 tcatcttagg atatgctttt aaaacaacgc cttttgctct agctgcagat ggagaaatgt
     1621 tatgtatatt ttgaaaagct ctattaaaag atgttggtga atcgtaacca tacttctcag
     1681 atagttctaa aattttgatg ttactgttct gcagttcaaa tgcagcctta gtcatacgtc
     1741 ttcgtctaat atattcagat actggtactt ccgcaatata tgaaaacatt ctttgaaaat
     1801 gaaaagttga gcaacatgca atttttgctg cttctgcata atctattcca ctttcaagat
     1861 tttcttcaat ataagtaata gcttgattta atttatttat ccattccatt taatattacc
     1921 tccaaacaaa ttatacagtt taataaaata atatacctct ctattcatgc acaaaattgt
     1981 aagcatttaa attcataagc attttctaat ccatcaatac aataaatgta aaaattatat
     2041 tatacaataa aaaagttcaa taacattata taatattatt gaacttctta taaaaattaa
     2101 tttaagtgta gttaaattaa gtattacact atcttatata ttacaatatc caactatctt
     2161 gtagtcttct ggcactttat aatctttatc tggagtacct aaattccatt ttaactcaaa
     2221 catagttgca cttattattg ttgcaaaata aagcataact ataccaatat ctattttttc
     2281 ttcatataaa ttagtatggc catcttttct aacagctaaa attacagtgc ctccatcaac
     2341 tataaatctc catggttgtc tatttaatgt agatggagct aatcttgcat aatgaaatgc
     2401 atcaagaagt gctctttctt ctaaaacttc tatgcttgca acatttcccc attcgttcat
     2461 ataaacaaca tcttctacac ctagtctaga agaaccctta tctgttacaa cttgcatatc
     2521 agattttgaa taattttgcc cagtttttgt agcatttaac actttagcat tatttacatt
     2581 ttctccatat ccaatagcta taattcctgt tacctcttta tctgaatcaa ttccaagttt
     2641 ttcttttact ttttttccat cttcaaaagt aacccaacaa gaaccaattc ctagttctgt
     2701 tactttaaag attatatttt ctcctatgta ccccgaattt tcaatataat tatcttttac
     2761 atcagataat attataatat aacttggaga atctatcata tttcctttgt atcctgctac
     2821 tttttctaaa ttattaaaga cttcacttct attcataatt cttatatcaa catctatttc
     2881 tggaactagt ttcttacaag attctgagta tttcttaatt tcactaagat attcctcttt
     2941 aatatctgtg ttcttatatt ctctaacaga ctttctattt aatataattg ttttataatc
     3001 cataatttta tcctccccaa tctagtcttg ttttaattat atacccatta atatattatt
     3061 ttactcattt atttaataaa taatgattac aattttgtaa ttattattta tcaataacat
     3121 tccactttta cattaataat gaccagttgt gtaaaatcca ttgccaacca ccaaataaaa
     3181 acatattata aaggtaatat aaaataacaa acaaatctta acaaaaaatt aaactttttt
     3241 tacacgaaag aaggcttccc gttgccttct ttatttttat aaaaaaataa gctgtttcta
     3301 aaatgatttc acatttttga aacagccaag ttattctata atgacaacta taatttgagg
     3361 atgtgaggga gagttgtcat tataaattta gggtaattat attatgtttt aagctagttc
     3421 tttctatatt tatataatac attttatata tgaattttat atgagattgg tataaatgat
     3481 atatgaaatt acatatgatt acattatatt aaattttatt attacatttt ccacaacaat
     3541 actcatatgt ctccacatta ttcggaatat ttacaaacac aaattcttta cagttctcac
     3601 aatactttaa tatagatata gttccacaag tgtaacattc aaatattaca caatcctcat
     3661 gttcctctac tatttcaaac tttttactat gttcaccaca ctctgggcac ttgtgattat
     3721 ttacattcat aaatactcct ccttattggt atagttacat gtcaaaaact taattaatgt
     3781 attctatatt acttattata tattctataa taaatcatat tctaatataa taaaacaaat
     3841 taaataaaaa aaaaccataa attttaaatt tatggattaa ttcttgtaat gataactcta
     3901 tttgaggatg tgagggaggg ttatcattac aatagggtat tatattatgg tttttaagct
     3961 agtctttcta cacttcatat aatacatatg ctatatgaat tttatatgat ttttacataa
     4021 atattttatg aaatttaaat tttttagtca ttttgaaatt tgtaaaaagt tctctaaaaa
     4081 tccacagcat aataaatagc caaaatttaa gtaaaacatc ataaaaaaat ttgtccaata
     4141 attttaacaa gtcacctcac ctcattataa ttattgacac aaacttaaat aaacaatcta
     4201 taaaccttct acaaaattta aattttcctc ttattgatac acaatctcta gtttgtcaat
     4261 tttattttgc atctctaaga aaatttcttc aacagaattg ttaaatatat tgcctaaata
     4321 gactctttta tctttttgaa atgaataaga ttttacatta ccattagaag aaatatataa
     4381 atgactccac tctgctccat attttgaaaa tctctccttt gtatctttaa acctcatatc
     4441 ttctattttt attgcttttt ctctgtctct tcttactaca gtatgacccc tttttattaa
     4501 actagttatt tttatatctt cttttaattt tatacgtgta tcatcatttc ttgaatatac
     4561 tttccatatc actttatgac gataattatc taattccttt attaaacttc tatcaatagt
     4621 aaagctatct ttagttacca caattaaatc aaaatggtct aacatatatt gaaatacatc
     4681 tctcatacct ttatttttta aaatatcctc accaataacc tctataaatc tacagccttt
     4741 ctcatacata cactttaata aacttgtgat ttcagaaaaa taattatttg tattattatc
     4801 tgcatctatt tctagagtaa agtacaaagg agtaatgtat gatttgtttc ctgttatctc
     4861 aaatacactt gatactgttt gtatattatc ttcaaaggtt aagtatacat tctcataaaa
     4921 cataaaaaat tccataactt tttctctcat attagggata ttcctatatc tattggataa
     4981 tatatttatt atgtcgtcag tagtattttt cccatcacat tcagccagta caacaatttg
     5041 cttccaactt aagatatcca catttccgat acttttttta tatccatctt cttcatcata
     5101 aataggacca attataatgt tttcttttgg ccacagatga accctagttc catcttttaa
     5161 tttcggatat ttatttctta cagactcata aaatctactt gtatcaccca ttattcttta
     5221 ctacccccca tattttaaat attctatgct aaaaatagca ctttattttt attaattcaa
     5281 caattattca aaattggtta ttaaatatgt ttaaatggat ttaacatcaa ccttatttat
     5341 atgttattga tgttcatata tgttatataa aagcttattt cttagacttc aatgtaattt
     5401 taactatttc tggcttacaa ttaagcctaa ttaaaacttt tgagccccct aagcctttat
     5461 ttaaaatcat agtgctatta tctttttcat atctgcccca atcatactta ggaaaaaact
     5521 ctctatttgg agataacaca cctccaacaa aaggcattct tattatacct ccatgaacat
     5581 gtcctgctaa aaccaaatct gctccccatt ttgcatattt ttcaaagaaa aatggcgtat
     5641 gtgctagaag aatattatac tcattttcat ttactttacc tagattactt tctacaaaat
     5701 tgtcacttag ttctaacttt ttattattag caaaaaaata tggataacat tccaatggga
     5761 caattaaacc atataaatta agatgactat tccctttata tattcttaca gattcatttt
     5821 ccagatttat tattttcttt gaatatagtt ccttaaaata atctttatat agatgtttat
     5881 atttttttat taaagatttt tgttcatgat ttccaattat atggtatact tcatattttt
     5941 caaccaaatt atctattaaa tctaatgcaa cttgaaaatt tttattttct ccatctacta
     6001 aatctcctgt tataaatata acatctggat ttaatgattt aattttatcc atcaacacct
     6061 tattgttttt atcaaatacc ttattatgta aatcgctcac ttgaactatt ttaaagttat
     6121 caaattccac tggaattttt ttgttgaaaa tagtatattc tgtcgtttct atatgctgag
     6181 tttcgtactt atacagaata tacaataata ttaatactag tactgtcatt gaaaccttta
     6241 taatcatatt ctcttcctct ttattctatt ttattaatat tattataatg cttttatgtt
     6301 ttttttaaaa caataacatc atcacataca tataaatctt ttcgacatta taaaaataag
     6361 ttatcctttt ctaaaatttt catttatttt agaaaagaat aactataaaa ttatctataa
     6421 acctctatat tgtgaaatat agtttttcct ttatttaaat ttttatatat atgctctaaa
     6481 gttccatcaa acctaataga atgcccagac tctaaaatat aagttttccc atccacatta
     6541 aggtcaagtt taccttctat tactagcaca tactcttcca aaacatgact atgtgtagat
     6601 gaaacatgag tacagttttc ttcaagctca attataaaaa gttcaaacct tctttgaggg
     6661 ctaaaagggt atattggata tagtttcata ttcccttgtt gctcaacaat aggctctact
     6721 tcttcttttt ttataacctc ataagtattt gtattttcat ttaatagtgt agtaaaattt
     6781 attttaaatc cagatgaaat tttccataag gtggatactg ttggacttga ttcacctctc
     6841 tcaatctgtc caagcattgc tttactgaca cctgtcatct ttgaagcttt atctaaactt
     6901 atgcccattt cttgtctcaa taatcttaaa ttttctccca cattaggaaa tttattatcc
     6961 atgttaaaat cctcctgata gctatttgta ttctatgatt tttacccctg taagttctac
     7021 tatctctttt aattttaaca tatgattgtt acaaatttca tcaaattcat atatataatc
     7081 ttcaagtcct aatgccttat atttatcgat tccaaattta tgatatggaa gtaattccat
     7141 ctttccttct ggaacattct gcttcacaaa taatgcagta tttcttatgt tttcttcact
     7201 atcatttact ccataaataa ttggcactct tataaccata gatttattca attttgaaag
     7261 tctacatata ttatctaata taattttatt acttactcct gtatattctt tatgaatatt
     7321 atcatccatg gattttatat ctacaaatat gtggtctatt ttatcaaata catcatttac
     7381 cttattccaa tcaaaatatc cacaagtttc aattgcaata tttatgcctt tattataaaa
     7441 aatatcaact aattctctta aaaaatcaat ctgaagtgta ggctcacctc cagaaaaagt
     7501 tatccctcca ttagattcaa agaaaaatat aaaatctttt tcaacttcct ttactatttc
     7561 ttcaacactc atttcttcgg tcattataca aatagcatct ttaagacatg ctttgataca
     7621 ctctccacat aaatcacatt tattatttat ttgtgttcta tcaaacagtg aacttatatt
     7681 ttgaggacaa acatctacac atctattaca agatatacat ttatctttca taacacctaa
     7741 tttcactata ttactccatg aatctggatt ggaacaccac ttacatttta atttacatcc
     7801 ttctaaaaat atagtagtcc ttattccatc tccatcattt actgaaaaat gttgcacttt
     7861 tacaactcta ccttttttac tcataatcta cctcataatt aaaaattact atgtgaattt
     7921 ctagctatta tagcatcttg catttctctt gataaattaa caaattgagt actatatcca
     7981 gcaactctca ctaataaatc tttatattct tctggatttt cttgagcttt taatagagta
     8041 tcatttgata tcgtattaaa ttgtacatga aatcctccta aggcaaaata tgatttaacc
     8101 atagatgtta gatttctaag tcctctttct gtttccacca agtcttgatt cagtcttaaa
     8161 tttagaagtg ttccaccagt atgaacatca tgatttattt ttgatgcaga tttcatagct
     8221 gctagtggac ttgttgtatc agtaccaaca aatggagaaa ctccctctgt caaaggtttt
     8281 gttgcttttc ttccgcaagg agttgcacct acgatttttc cagttggtac atagtttgat
     8341 atacccataa atgcactatt aaatttagaa ccaaaaatat ccttgtattt tctagtttca
     8401 gtataataaa aatcagaaac ttcaattgct aatgaatcta cataatcatt atcatttcca
     8461 tacttaggaa catctaacgc aagttttctt aactcttcat aaccttccca atcattattt
     8521 agtgctttgg ttaattcttc caatgtagta accttatctt caaatactaa tttttttata
     8581 gctgccaatg aatttgaaac tacacctaat cctataccag ttaatacagg tcctatatta
     8641 tattttgctc ctcctttgct taaatcgacc cccttatcta aacatccatc tacacaagta
     8701 gataaaaatg gtcttggtac catttcttta tgaatttgtt gagcaacagt agttcctata
     8761 acagaatttt ttacaagata tgctaattga tttaaaaact catccttaag ttcttcaaaa
     8821 gaagtaaatt ctgtgatatt ttttgtatct agacccattt tttctccagt taacctactc
     8881 ttaccttcat tcatagcata ttctaaagct gctgcaaaat tgatatttac ggctgaagtc
     8941 cactgtcctg tttttctaaa atgaggaact acacatccac agttactcca atctctagcg
     9001 tcttctgcat catatccatc ttgtagtaac atttgtgccc ctgcattatc gctgtgtata
     9061 gcagggaagc cagttccttg ttttactaat ttagcaactg ccatgacaaa attatctggt
     9121 gctccttgac tcaccctaac acttaatcct ggctggtgag tttttacact ttctgtagcc
     9181 tttaaacaca tatatgagat atcattcgtc ccatcagtac catttctctt tttaccacca
     9241 acagtaagat tttgaaattg attatatcct gcaaaataat ttgccgtatt tgctgatata
     9301 gtccataccc attctgataa cttaatccaa agtgcttcta tcaattcttg agcttctgat
     9361 tcagttattt tgccttctct agcgtcgttt tcataatacg gatacatata ttgatcaaat
     9421 ctacctaaat ttaatgccaa tggattttct gatagaatac cacctagttg aacaaaccat
     9481 acaaattgta ttgcttcata aaaattagtt ggagggtttg ctggtacatt catacatatc
     9541 tctgaaattt ttataagttc taactttctc tttttgtctg tttcttgttc actcatctct
     9601 tttgcaagat ttgaatatct ttgtgccaat gtcataatac cctgtgcaac tatagaaaca
     9661 gacttataaa agtctatttt ttcaatattt tctgaaatcg atatatctaa ttctttaatt
     9721 tttttatctg catcttcctt aatcccttta tatcctttta caaacagtat atcttgataa
     9781 tctggtgtta cctcaccaac tgcctgtctc cacttagaat cattatctat tattcctgta
     9841 tctactaata ccttagctgt atcttctggg attcttgcta aaaaaacttc ttccaaagat
     9901 tttcccttcc aatatggaaa tatttctttt cttataaatt ctatttgttc ttttgatatt
     9961 acatatgggt cttgagttct ttcatcaaaa gtatccattt ctttgtctac ccattgccaa
    10021 gaaaactctg gtgttaatat ccctgttctt ctaaattttc ctgctgtacc tactattaat
    10081 tcattttcaa agatttttat aggaagctct ttacatgtct ccaaaaaagc tttagctctt
    10141 cttatacaga tagcttctcc ctcagttttt ttatgtgatt cagtaaaaat cctagctctt
    10201 tcacaacata tcataggttt agaattaata tattcttgtc taaatgcttc tattctatta
    10261 gtttgactca tttaaaatcc ccctttggat aaatatatac ttatatcttt gtatgttata
    10321 acatacttta attatattat actgtccttt agcataaaat ggaagcaaaa taatttattt
    10381 tttcatctac cttgaacacc tatatattaa tacttttttt gctaaatttg taaaatctaa
    10441 aaaatttgtc tatgatatat tttttatatt gttatagtta tttctagtat actaacgatt
    10501 tgaaatatca aatttatata ataggcttag ttttaaaact ttagttgatt gtttaatttt
    10561 gttaacttca acatgtatat ctttcatata aggaagctct acaacaccta ctcccttatt
    10621 aaagtcttct tcaaccatag aaaaaatttc tttctcagtt aagttcgtat tagtcaatga
    10681 cttaaataaa ttatatataa atttaatata tttatcactc tcagataact tctcatcttc
    10741 tggatactct ttagcacaga gaatatctat tgttactttt ccttctttat catcttcaga
    10801 tacagaatag aataaacttc taatattttc ttcaggtggt ttttcaacac tatttataga
    10861 cactacaaag ctaccatctg attttgaatt agtaatacta taaccactgt ctattatggc
    10921 tttcgaaaat ctttgagata aatctttttt tacttcttct ctacctttca catcattcat
    10981 cttttgcgag taattattaa ataactcaat agattcatct tttttatctt caactgattc
    11041 ttttaatgaa tctttactaa tttgttttga actacttaag ttcttatcag atacaggaga
    11101 cttacatcct accatcaatg ctaaactaag atttaaagcc acaactattg ccactttatt
    11161 tatatttctc tttactttca tacacaatcc tcccatcaga ctaacctctc taaaacatta
    11221 ttcattgtat tagaatatgt ttatattctc tttatattaa aaatttatta ctcaacacat
    11281 atcaccttcc tatctaattc ttatagatta ctattagtaa aattataaat tccttaatac
    11341 aaaaataagg atgttatctt ttgttattaa aagataacat ccttgttttt ttgtatcaat
    11401 acatatgtcc caactagtaa cttaatcgct ccaactgtca gtatcttggt ccaatggtat
    11461 gtatatattt agtataaatt tatccatact attttcaaat gataattctc cattatattt
    11521 ttgtaaagaa gatttaatac tttgaatacc tattccgtgc acaaacttat ccattttgtt
    11581 tgttaacaat atatttttat tgaaagtaat cttatttagt ttactatttt cacatttaag
    11641 tacaaaaaat gactttgtta tagttccttt aattctaata tatttattaa tattttcatc
    11701 caatatttta tcacatgctt ctattgcatt atccaaaaca ttagagaata tacttgaaac
    11761 atctataggt tttacaaaat ttacttttga aaaatttata gagcaaataa atttaattcc
    11821 cttttttaaa catatttcac tcttttcatt gattataatg tctaatatca tatttcctgt
    11881 attataaata tatttaaatg cttttatttc atcttttaaa ctgttaacat attcgtcaat
    11941 ttctttgcta ttatttctaa gcttgtctat acaataaatg tgattattta tatcatgata
    12001 tagtttctta actcgcatat gagattcttg aatacttaaa taatgtgcat attgcatatc
    12061 taacttattt tttattaatt cagcttgaga ttttaatcta ttttctttta ttatttcttt
    12121 aaacaatagt ataagaaata tactactagc tcctaccaat ttgggattca caaaattatc
    12181 aaaatagtat ccttctgaat gtaatgaccc aaaccgttct aattttttta atacataatt
    12241 tacaagcata ttcattgtat tagctaataa ggcaaataat aaatatacat aatttctctt
    12301 atcttgacta attaatttta tagacttcct aaaaaagcta aatataagat atataagaat
    12361 aattattaaa ttactatata tctgaatttt tatagattct aagtgaggta ttaataagga
    12421 ttctttagat gaaataacat tacctgtaat tgcaaaaaat agtattctat atataacagc
    12481 atccaaacat tcataatata cactaaatag ataaaagaaa acagaatact taatataacc
    12541 tatcttataa aaataattat agtatcctcc ccaagcaata aatgctaata tataaaataa
    12601 atagtttata ttactaaagt aatttcctcc attaaataat ctacttatct gaactataat
    12661 gtaaataaga aacaaaacaa tataaaatac tattttttca ttttttttac tatccttttt
    12721 ataaattaaa attccgttca ttatagaaaa ctgtataaat atagataaaa ctactaatag
    12781 ctcagtcata aaatttagca tattgtatca ccaagaaatt ttaaaaattt ctccttaact
    12841 tctttatatc tatatctact aataggtacc tcttctccac tttccaatat cacagtatta
    12901 ggctttatat tttcaacata tcttaaattt actataaaac ttttatggca tcttataaat
    12961 ttatatggat ttaattcttt ttctatttta cttaaactat attttatatc aaaattctta
    13021 tttattgtgt gtattagcat atcttttttt tgaacttcta tatattttat ttcatttaaa
    13081 taaatcttat atgtattaga cttatttttt attgtaatat gacttttctt atttatttca
    13141 atatctttga tacaagttaa cacatgtttt tttagatctt ctagctctat tggctttagt
    13201 aaatacctat aagctcttac ctcatatcct tcttgcacat aatctattaa tgaagttata
    13261 aatattattt ccatttcatt atctacttct ctaatctttc ttgcagtatc cattccattt
    13321 agtttttcca tttttatatc aagaagtaat atatctatat cttttaaatt actttccaat
    13381 agatcttctc cagatagata cttttgtatc tcatattcta tatttatatc ttctaatata
    13441 ttctcaaggt aatcttctat taaaagttgt gtttcttttt catcttcaca aactgctatc
    13501 ttaatcataa aatgcctcct tacataattt ctagttaata caattaaaat ttttataata
    13561 tatttaaaga taattttgta tatctgatac tttaggataa cattttcaaa ttatttttat
    13621 caatactcaa tcctatacaa ctgttatttt gatatctcaa tttaaagaca gaaattacta
    13681 taattaaaaa cagtacttac tgataaaaga agatgtctta aaatatcaaa aaaataattt
    13741 taggacacct tcttttattt aaacttttta agccttaaat aatacattaa tctcctatat
    13801 aattgtactg catataaaat caacaatata atctgtagta caatagcagt tacagatgct
    13861 ccacaaccac tatataaatt tcttatttca atattcaata aatatataaa tattactact
    13921 aacataaaga atataaacat attcataaat aaaattgact ttactttatt ttttttatca
    13981 gaatcactat ataatttagg aggtatgtct ttgtattctt tcgaccatat ataccatgca
    14041 gatgtaccca taaaactgcc aatactccta aacacaagat tccagccatt tgatttatgt
    14101 tcatcaaaat aattattctc tgcatcatag tagtaatcaa cagtgtattt tatttttctt
    14161 ggactttcct tatgaaaata aaatattcct ccaaatttat ttatcctata taaattatat
    14221 cctttagctt caatatcttc taaccatttc tctatcttat caggtgaact ataccagtca
    14281 aatttgaatt ttattttctt ttccataaca ctcattctcc aaaatgatat ttttaaatag
    14341 cttaaaaatc tatttaaata attatatcat tttgatacta attttaggtt acaatcagat
    14401 atttaaaaag ctatttaaat agtaataaac taatggccat aacgcccatt cctgcaatca
    14461 atccataaat ggataaatga tgttccccgt actctctagc tgatggtaat aattcatcta
    14521 gtgatatgaa taccataatt cctcctacaa ttgcaaatac aattcccaga gttatatcat
    14581 tcaaaaaatt tctaagaagt acatatccaa tgatcgctcc taaaggctca gacataccag
    14641 ataaaaagga ataataaaaa gctttctttt tatctcctgt ggcataataa actggtacag
    14701 atacagatat accttctggt atattgtgta ttgcaattgc aattgctatc ggtatagcta
    14761 ttgtaacatc atcaagagct gatataaatg tagctaatcc ttcaggaaaa ttgtgaattg
    14821 ctatagccat tgctgtaaat acacctgttc tcaaaagaga cttatttttt ttgatattct
    14881 tttcttctat attctccatt ttctttattt catgtggatt ttcttcttgt ggaattagct
    14941 tatcaataac agcaatgata gccataccta taaaaaaagc tattacagtt gaccaacctc
    15001 ccaatttttc tcccatagat tttgtcaaag catcttgtgc tttaggaaaa atttctatca
    15061 tagaaacata aatcataaca ccagcagaaa atcctaagct tatagaaaga aacttcgtat
    15121 ttgttttttt agcaaaaaat gctatacaac ttcctatacc agtagataaa ccagccaata
    15181 atgttatcaa aaatgcaaat attacattat tcaaataaat caccttcttt ttaattgata
    15241 ttgattatca tttcatttat gctatcatat atattttata tagtcaatat ttatcaaaaa
    15301 tatacacaat aaaaaagcag agatataacc ttagtctctg ctttttcttc atagtatatt
    15361 aaaattattt attcaagaaa tccactgcat attgcacata taaagcagtt ccaatttcta
    15421 gggcatcttc atctatatta aaacatccat gatgatgagg ataacaagca ccttttgatt
    15481 catttctagc tcctacaaat gctagtgcac caggagctat attcataaac tcagctaaat
    15541 cttctgctcc tgttactttt tcaaatagtg ttatacagtc ttctccaaat agcttaattg
    15601 ctgcttctgt agctatcttt gaacattctt tgtcatttat aactgctggt gttaaataac
    15661 catattctaa ttcagcttct gctctatatg ctcctgctgt agattttgct atacgttcta
    15721 aaattccagg aattttcttt ctaagctctg gattaaatag tcttattgta ccttctaaga
    15781 cagcttcact cgcaattacg ttaaatctag ttccagaatt taaaactcca acactcacaa
    15841 ccaatggttc cagaggactt acttctctac taaccataga ttgcaaattc ataactattg
    15901 ctgaacttgc aagcactgca tcaactcctt gatgaggaag tgaaccatga ccacctctac
    15961 cttttacagt aattttaaag aaatcagcag aagccattct tggtccttct tctactgata
    16021 tagttccaga ttctacatca gtccaaagat gtattccaaa cacactatct acaccttcca
    16081 tagcaccatc ttgaatcata gctcttgcac ctttgccaac ttcttctcct ggctgaaaaa
    16141 ataattttac agttccattg atactatctt taatatcatt taacactttt gctgccccta
    16201 gaagcattga tgtgtgacca tcatgaccac aagcatgcat aagtccttca tttttagatt
    16261 tgtattctac ttcagtacat tctacaactt gtaaggcatc catatcacct ctaagtgcta
    16321 cagtctttcc tgaattagcc ccttttatcg ttgcaattac acctgtacct ccagctgata
    16381 catatggtat tcccatttta tcaagttctt ctttaaccct ctttgatgtt ctaacttctt
    16441 ccatactttt ctcaggattt tcatgaaatt cccttctaag cttaattaca tagtctttat
    16501 aactctttgt tatttcttta atatccatta cagacctcca agtagtacac attcttctta
    16561 tttaaaatat tttttattag ttaaatacta tattagattt acaaagatac ccgctattac
    16621 tacagaagct attgtaactg tagtaaatcc accaacaagc atctttggaa gcattttatc
    16681 cattaaaaat tctttttctt ctggtgtttc agccagagcc tttactgctt cttcagttaa
    16741 tatatagttt ggcggaaaac cgtaaagtga tgtaagtgaa actgaaaaag ccatttcttt
    16801 gctttcacct aaaagctttc ctgctataat agaaaaaatt cccataccta ttacaccaaa
    16861 tattatgatt cctacaagag gaactgccat ttcaacaagc attgctggtg tagcttttgc
    16921 taaccctgca aatataaaag ccatcaatga tgtcattaac catccaaatg aaccagattt
    16981 attaagtggt tttctatcaa ctattccaag ttctgatgct attacaccaa atattaaaca
    17041 cataacatat cttgatacaa cttcatttgt aagatttgta aattgtactg ctaaccatgc
    17101 tacaaaccct aatctaagta ttatcatata tgtcgtatca tatttttctg gtaagtctgg
    17161 aattatctta aatctgcttt tttgttcagg taaatcctta actttttctt cagctgtatt
    17221 tacagttact tttccacttc taaaatccga taaaagtctt tttccttctt ttttaagcat
    17281 taatgctgta ataggataac ctgcaaatcc ttgtgcaaca tacattaaaa ttgcaagtac
    17341 tgacaaactt gcaagacctt ttgcagtagc agcatctgcc atcattatag atgcaactag
    17401 ccctccagta agaggaggtg tagctattac tgctgtatcc catccaaata ctactgtccc
    17461 caatgccaaa gcacctacac atattcctac tattcctgcc aaggctatta cgattgtttt
    17521 ccattgacct gctaattcct taatactcat catagttccc atatgagtta ttaacaaaag
    17581 catacctaat tgagctagtg gtgttcccaa agcggctaag tccacaatat tttctgggaa
    17641 aaaagtccaa aatcctacta taaatataat tgctgataca aaaaccgacg gaacaaaagc
    17701 tttagttttt gctgaaacta catctcctat cgtcagagat attaaaacta aacaaaaagc
    17761 tgttattgcc tccatacaaa atcccccctc ataataaatg tatttttata aaatgtatgt
    17821 atagttatcc attattttac tacttttgaa aaacatttca agtcaaattt cgtttttttt
    17881 cataaaattc tataaattct acaaattaaa gttagttttc taaattattc tttttataaa
    17941 aacgttatcc ctcggattgg ctattttact ctatttttac aaggagaatt aagtgtaatt
    18001 ttcaaaaaat aaaacataga acatatttta ttcataaaac cttccattaa tataaaatga
    18061 attgttttta tgaatcttaa tatatcctat gtttgtaagt ttaatgcgta tgttagctat
    18121 agtgtaaaac ttatatacta gttattgtgt aacctactat atactccatt tttgtttact
    18181 aactcttcat gagtacctct ttcttcaatt cctttatctg tcaatactat gatttcgtca
    18241 gcatttttta tagtagtcaa tctatgtgca acaactaaat ttgttctatc tttacttaat
    18301 tcttctaaag actcttgaat ttctctttct gtgacattat ctaaagcaga tgtagcctca
    18361 tccaatatta ttattggagg attctttaaa aagattcttg aaattgaaat tctttgtttt
    18421 tgtccacccg ataattttac tcctctttct cctatataag tatcatatcc ttctggtaaa
    18481 gtctctacaa agtcatgtat cctagctttt ttagcagcag ctatcatttc ttcatcagtt
    18541 gcattagggt tcccacaaag aatattatct cttatagttc ctgtaaataa aaatacatcc
    18601 tgttgaacta tacctatatt ttttctcaat gattttaaac tgacatctct tatactcttt
    18661 ccatctattt ttatatcacc cttatcaaat tcataaaatc ttggcaacaa attacacaat
    18721 gtagtctttc ctcctccaga aggtccaact aatgctatag tcttaccagc ttttatagat
    18781 aagctcaaat tatctaaaac ttgagtctta tcttcatatg tgaaagatat attttctatc
    18841 tcaatatcac cttttacatt ttcaagttca atagggtttt tagcttcttt ttgatggtct
    18901 tgttccatta tctctataaa tcttttaaaa cctgtcatac cattttgata ttgctctgta
    18961 aagtttatta attttttcat aggttgtata aacattttta catatagtat ataagcagca
    19021 aaatcaccca aagtaatttt tcctatataa gtaaagtaac cacctgctat tacaactacc
    19081 cactctaaga tatccatgta caaattcata ccagagtagt attctgccat tactttataa
    19141 gaatcttgtc ttgctcttct aaataattta ttgcttctta caaacttatt taactcttct
    19201 ttctcagtac aaaaagactt agttactttc atacctgcta tactattttc taaagtagca
    19261 tttacatcac ctatagtgac tctactcttc ataaaagcaa tattcatctt atctttttta
    19321 gctattgcaa accaaattat aaatggtaat atagcaaata ttataagcgt aagaggaata
    19381 tttatatcta taagtataaa gaatgaacca accaacatta ctatagagat gaataaatct
    19441 tcaggtccat gatgtgcaag ttctgttata tccattaagt catttactat tctagacata
    19501 gttactccag atttgttatt atcaaaatac gtatttggaa gtctttgtaa gtgtgtaaat
    19561 acttcatttc tcatatcagc ttgcatatct acaccaataa catgtcccca atactgcata
    19621 aaatagttaa gccctgcttt tgctataaat ataagtataa gtgctactgc aaaaaccact
    19681 aacattctta agtttttatt tggaacaacg tcatccatta tatttctagt tatcatagga
    19741 taaactaaat cacataagga aaataaaaaa gctgctataa ggtctagtgt aaaaattttc
    19801 ttatatggct tataatattt gacaaattgt tttatcataa gtattaaatc cctcctttat
    19861 tagtacttct aaaaaatacc cataaattag tataacataa caataacttt aaatctttta
    19921 taaacttata aatgaaaaca atatttttca taaaattgaa tatttatcat atagttgtat
    19981 gcttgaaata acataaaaag agctatatct ctataaataa aaatttataa agatacaact
    20041 ctatatttaa tactgaactt tttttataaa tacaaaacta cttcaatgca ttcaatattt
    20101 ttttatccaa tgccgttgta tttatacctg ttatatatcc tgttgccttt attgtaggta
    20161 tatcataagt tgttggtagt atagtagtag atacgattaa atcagcacct tcttgttttg
    20221 ttgaacattc agcaatttta cattgctcaa tttctgcttg tatattattt tctttaacca
    20281 acctttctac tctgtcacaa actattgttg atgttgcgat tcctgcccca catgctacta
    20341 atattttttt catatattgt cctcctacac tttcttcaat attttataat ttaaaataca
    20401 actgataaat ttcctatatc tttatctata taaataatta tatcatttta tatcaaaaga
    20461 acatcttaaa attattcttt tagtatgttt tttatttcaa cattgcttat acttaaaata
    20521 tcatttttta tacaagactc tatgtacttt gatatatttt tatagtcatt taaatcaaac
    20581 tgttttatat tcaagttata ttttatatca ctaacaatac ctaccaaatc agacttatta
    20641 gaaaaaatat cctcatattt atcttttcta gttacttcca atcttttaaa tctatagttt
    20701 ttatatcctt ctatgataat taagtccata tcttcgtata attgaatcat tttatataat
    20761 ggactcttat cttttagttc ttcaatatat gctactcgtt tatctgatat tatacagact
    20821 tttctcgccc cagattttct atgtttgtaa gtatcttttc cttcctcatc catatcaaaa
    20881 tcgtggcaag tatgtttaat aactcctaca cgataccctt ttttatttaa ttcttttatt
    20941 ataccttcaa tcaatgtagt tttacctact cctgaatagg agacaactga caatatatta
    21001 tacataaaat tgtgtccttt cataaaacta tctaataaca atatcgacta attgattttc
    21061 ttttattgtt tcattctttt taatcttaat catacaatta cttcttagag ttgcagataa
    21121 tctagctttt gcatctttct tatcatttaa ctctacatac aatttccctt cacttgtata
    21181 tagttttcca tttacaaatc tatctttatt tgaaagttta ttaaattctc ctttaaatat
    21241 agcttttata taatgtattc ccttcttata tgttaaatac tcaataacag gaactaccac
    21301 attatcaaat acaatgattg ctgcatgtgg attaccagaa agaccaaaca atagcttttc
    21361 tcccaatgta gctacataac aagctccacc aggcttaatg tttatcttcc aaaataattc
    21421 tgttgcccct atttttttga atacatctgg aattaaatct ttatctccaa cagagacacc
    21481 acctgttgta attaaaaaat ctgcattctg taacatatcc cttacctttt tttctaactc
    21541 caatatatca tcagaacaat tgcatattgt atctgcttgt attccaagtt ttaataatct
    21601 tgaatataag gtatatctat tactatcgta tatcttacca ttctctaaag tctcatttat
    21661 atcttgtagt tctgtgcctg tattcaaaat tccaactttt aaatcatcat aaacagttac
    21721 tttggatatt ccaagacttg ctaatactcc aataccatca tatcctatat tctggttttt
    21781 attaataatc aattgcccct tttttatatc ctcaccctta aatatataat tatcgtattc
    21841 ctttaatttt acatttaaag ttatcatatc acctataacc gttacatctt ctttttttat
    21901 tacacagttt gcaccccttg gtatttttcc accagtcatt atcttcactg cttcaccttt
    21961 atttaatacc ctgttaaata cttctcctgc atacaaagtc cctataacct ttaaatttct
    22021 attttcagaa tttgcattat atgcataacc atcatatggt gacctatcaa atggaggact
    22081 atcaattgtt gaatatatat cttctgcaac cactttattt actgcatcta tgagatttac
    22141 tctcttgacc ctgtttattg gtaaaacatt tttttcaata atttttatgg catcttctaa
    22201 ttctatcatt ttaaatactc cccaatatat tttttattta agatatttct atgttaatat
    22261 attatatatt acaaaatttg gaaatacttt tatcttatct ttaattataa ctaatagtta
    22321 tctataagtg cagttcaaat tttataaatc taataaaaaa actgttccca gctagaaatt
    22381 tccttctatc ttgaaacaga atcgtatgct ttagaatatt ttattctaat aaagttcctt
    22441 ttattatttt cacaatttta tcactttcaa tagtttttat agattttatt ttgttatcca
    22501 caatacttat tgtaagtacc tcttttttta atattttgtt atcttctttc aatctatagc
    22561 tcttaactac tttactacct tcatgattat atgctgttaa atcataattc gcatctccta
    22621 caatttcagt tattctagac tcttcaacac caacatactc tcccaggtca atacgtctaa
    22681 aattttctag tgatacagcc gatttttcat ggtcactttt ttcaatcaca atactagatt
    22741 cagcatcaac aactgcaata ccttgtgatg agaaagaatt agtttcaaca ttgacaactt
    22801 tatttccgtt aatatcaaca tataaagcac ttccatctag ttcgtcattt ccagtatatt
    22861 ttggataaac taatgtcata taacttgatg tatttatatc ttgcattgta tttgattctt
    22921 ttaaaacttt aggttttata taataaaatg tattataagg tatacccata ttctttataa
    22981 tatacttata atctttattc ataatttcat taacatcatt ttgaactttt gtcatagttt
    23041 tttgctgtgt tgctttactt gctacttgat ttttctcttt agtattttct ttatgacatc
    23101 caacaagtat aatacctaaa atagttacac aaactaatga cctttttaac ttcataaatc
    23161 aatcctcctt tcttaaattt tatagtattt atctatatat caatttacag ttatctcttt
    23221 tttcaataat tatatttatt atattataaa ataattatat aatatttatt tgaatttaat
    23281 aagattaaga agctaattgt aatttttctg taataaaaca tacttaaagt tgaattttga
    23341 aaattcaatt tacgatattt ttattattaa gtaccttaca acattatacc aaattattcc
    23401 taaacaatca aaaactccat gaatatttaa aggttttcta ttttaaaaat cacctttgac
    23461 tattaattta tcaaatatgt tataatagtt ttagataatt taaggaggat taaataatgg
    23521 attttaaaca acttgaagta tttgttgctg ttgcaaaaca tcagagtttt tcaaaggctg
    23581 caagagaact ttttttaact caacctactg taagtgcaca catacagaat ttagaaagag
    23641 agttggaaac tgttcttata aatagaagca acaaagttat tacactcaca aaatctggtg
    23701 aaattcttta tgaacatgct atatatatat taaataattg taaacgtgca atttatgata
    23761 taaaagaata ttcaggaaaa atagaaggta ttatagatat agcatgtagt tccatccctg
    23821 aaacttatat tttacctgat tttatgaaga gtttttctat gagttatcca gatgtaaaat
    23881 tttccataag ccattatgat tctcaatatg ctatatcaga aattttaaat gaacgaataa
    23941 gctttggatt agttggttct aaaatcaata atcctcaaat agagtaccta gacttattag
    24001 atgatgaact tgttcttatc actccttcag attttaaaat tgataataaa aataattgta
    24061 tcgatatagg agaattggct tatctgaatt ttattatgag aaaagaaggg tctggtacaa
    24121 gaaacttaat actaaacaca cttagtaaaa ataactttcc agtaaataaa ttaaatgtaa
    24181 tagctcatgt agaatcaaat gaggctatta aggaaatggt taggttaggt cttggagtat
    24241 cctttatatc gtacatttca gctattgatt atctcaatgc aggcaaaata aaatgctata
    24301 aaataaagga tgttgatttt actagaaaat ttttctttat ttactctaaa aagaaaactt
    24361 tttccccatt agaagataaa tttctaaaca gattatgtga gtatttcgaa atcataatat
    24421 aataacttta ataaaacatt gcttaatttt agatttacaa ccaattaata atctaattat
    24481 aactatcaca taaatactta taaattatta taaatcatct ttctttttta tttaatttag
    24541 tcaaaaaatc cctcattcaa atgaacttgg gatttttttg tttatactac tccaatagca
    24601 gctaatacta tacaaataat cataactgaa attagtatcg tagttgtctt tacctttttt
    24661 cctaataacc aatacataat accgaatatc atagctggaa gcatacatgg cattatttgg
    24721 tctaagtaag tttgaatagg ctctgcccat tctccactac ctacattaac actaagtttt
    24781 aacattacat tagatgcagt catacctcca attaccataa gtcctaacat tgaagctgct
    24841 tccataactt ttgaaactat acttgaacca ctcacatctt taaagaattg agttccatac
    24901 ttaaatcctt tatctagaca tatataacgt ataataaaat gaggaacatt tattattaaa
    24961 aggaacaaaa tcggtcccat tatgttgccc tgagaagcta aagctatacc aactccagct
    25021 gctataagct ttaatgttcc ccagaaaaat gaatctccta ttccagaaac tggacccatt
    25081 aaacttgatt taacagcatt tatactatta gcatcaaatt ctttgttctt tgcattttct
    25141 tcttccatag ctccagatat acctaccaaa agagtactta tatgaggagt aactgacata
    25201 aattctaaat gtctttttaa agccttagat ttcttttctt tatcatcttt atacaatcta
    25261 cgtattattg gtgccatcat gtatgcatat gctatatttt gcatacgttc gtaattccaa
    25321 gatgaatcta atgtcaatga ccttctaaaa catttccaaa agtctttcct cgttatttta
    25381 ctgtctggtg aaattgtctc taacttctta gaactcgttg tcatctacta ttacctcctt
    25441 ctctcctaca aagttaacat atattaaggc tattgctaat ccaaacaatg caactcctgt
    25501 tactgttatg tttaagtaag caaccaatat aaatcctaat aataaaaatg ctactgtttt
    25561 cttattaagc atcattctag ctaacatggc aaaacctatt gctggtatca cacctgctgc
    25621 aacatccatt ccatgagtta catatgatgg tatgaatgaa agtattttct ctattactgg
    25681 aacacctaat gaaaatccta ttcctactat tattgctaat atcaatttat ttcctatacc
    25741 aaataataat tgtatacgct ctacaccttc aatattcccc tcttctgcat aacggtcagc
    25801 aaggtgagct ccccaagtaa gtactactgt catcaagaag ttatctataa gcaatactag
    25861 agttgctata ggaactgcaa gtacaactgc tgctgataca tcttgaccag ttaatattac
    25921 aaatgctgtg gcaataatag acccagatac aaaatcagga ggattacttg ctccaacagg
    25981 aaatgcaccc ataaatacta attctaatgt tgaacctatt actatacctg tgtgtaaatt
    26041 acctagtgct aaaccaacaa gtggtgcaac aactataggt ctattatgca ttgtagaacc
    26101 tacaaaacta tgcatatatg caaagaatgc aattagagtt atcaaaataa cttgtgtaaa
    26161 ttccattttc atccccctaa aattatatta atttcattac atttgtagcg tcatcatcag
    26221 gaactaactg tacaaataca tttacgcctt ctgaatctaa ctctttaatc atatttatat
    26281 catcttttga tacatgtact gctttagata tattttcacg attatcatct gattttgttc
    26341 caccaaggtt tatagattta atagctttta cctctttagc aagtctataa acatcttcta
    26401 cagattcaca aagtatcaat aagttatatt tatcagtaac acctgagtta agtgctttta
    26461 tagaatctgc tatgcttttc attactagct taacattaga tggtttagcc attttaagtc
    26521 ctgccattcg taactcatct tttaataatt catcacttgc tattaatata cagtctgtgc
    26581 ttaaaaactt tgtccacgta aatgctactt gtccatgtat aagtctgtgg tctactctta
    26641 ctaatttaat cattataatt cctccctaat ttttttgatt tcatccatga atgtttttac
    26701 gtgttctaca ctaacatccc caaaatcttt gtaattatct ttaaagtaac ttcctattat
    26761 tgccatatca cctaattcaa gctgtttttt tgcattttct aatgtaactc cagcagctat
    26821 aaccaatggg aaatctccta aatttttacg aaacagttct attttttcca ttgatgtttc
    26881 ttgacctgta gcattttctg taactgctat agcatcacac cttgtcattc ctatttttaa
    26941 atcttcttca atattatttt ctgataatac tggttgatat ttaaaacgta cgcctccgat
    27001 taaatatgct ggacatttcg aacgttgtaa tttaaaaaat tcttctaaag tagcctcatc
    27061 tctaggttta acatgaccta ctacagaatc aacctgtata taactggcat tatattttgt
    27121 tgccagttcg aatcccatag tatcaacatt taaacagttt actccatatg gtattgataa
    27181 attagctttt gatacatact caagtattct ttctaaatca taataattac catagtaatt
    27241 ttctaacatt attccatcta caccattttc ttcaaaaatt gcaatctctt ttttagctct
    27301 ttcaaagata tcttctggag tgtctccttt taagtgtatc attgctataa ttggcttttt
    27361 acttttaaat actgataata ttcccttttt tttcataaac taaaatacct cttcttcttc
    27421 tttaatttgt aatgtcacgc tttgtattcc tcttctagat tcttctacta attggtctat
    27481 atcttcaact gttaattcat cagaaaatct tgtaagaagt tctagtacca tattcaaatt
    27541 cattccagaa aataacttaa cattatgtag attagttaat cttaacattg ctgtacatac
    27601 acttgctcca tataagtcag ataaaattat atattcacat tctttatctt ctgatattgt
    27661 tttttctaat tctaaagcaa aatctgaagc actttgtcca gggtataaac catacgaact
    27721 aacacaatct gctaaatctc ctactatcat ttttactgca tttaaaagcc cttttgaaaa
    27781 gtctccatgg gatgctaata ttatcttttt catacttatc ctcctactaa atataaatta
    27841 tatttctgct gatactgcat atttgtactt atcacttctt ataaattgct cattgtaata
    27901 taatacatca ttattttctg tatacaccga ttgtcttact ttcaatagtg gcatattact
    27961 tgcacattta agatggtcag aatattcttt gtttgaatgt tttacctcaa atatattttc
    28021 tactcgctta ggtaatgtgc ttatattatt agatataacc tccattaaag atttatcttc
    28081 tagttcttct tttaataaaa acatataatc tattctaaaa taatcaactt caaaaataac
    28141 aggtacttca tctattaatc taagtctttt gatacatatt accttttctt ctagttcaat
    28201 acttaaagct tcagctactt gcttatcagc tttttttatt aaaacagaga ttattttagt
    28261 gcttggtttt gcattaatac gatgacaaga attagtaaaa ctatttcctg cacatgttgc
    28321 ttctattctc tctggtattg ttacataagt tcctttacca cgttttttac ataatatatc
    28381 ttcttttaca agttcattga gtgcatttct aactgttata cgacttatat taaattcttt
    28441 acataagtct ccttcagatg gaagtttatc tcctggttta tatatgccag atgctatttg
    28501 tttttttatg ttgtcatata gttgttgata taaaggaaca aaggaattta cttctaaatc
    28561 catttctttt ttttgtctct tttcatccat tttgctcacc tccctatgta acatttttat
    28621 aacattatat aacaagttct ttattacata ttataacaag atgaaatcgt tgtcaatttt
    28681 tttttaaata atttttaatt tgacagtatt ttattttatt ttctccagtg tgacatattg
    28741 ctatttttac aaaaaaataa gactgttgta caacaggtta aattgtttat acaacagtct
    28801 ttattaactt attatgctta tatttttact tataaattta tattaaaaaa tgccttatat
    28861 cccttataca tttcatatat gtctacttta gagacaatct catgtggtgc atgcatattt
    28921 aaaactccaa caccacaatc tataacctct gcaccttggt ttgccaatat atgggctata
    28981 gttccgcctc ctccttggtc tacttttcca agttcagcag tttgccatac tacatttcct
    29041 ttattaaaaa ttctctttac ttctgacata aattcagcat tagcatcatt acatccactc
    29101 tttcctcttg aacctgtata cttagttaaa actactccat ttcccatata tgcagaattt
    29161 ctcttttcat acgcttctcc aaaatttggg tcatatccag cagaaacatc agctgataat
    29221 accttactat ttgctaaagc ccttcttact ttaatatcac aataatctcc ttctaaagca
    29281 atcagttcag ccacagtatt ttcaaaaaat tttgagtgca tacctgtatt tcctttagaa
    29341 ccaatttctt ctttatctac acataatgca actgaagtaa actctggttg ttcagtttct
    29401 aatatagctt ttacagcagc atatgaacac accctatcat catgcccata tcccaacacc
    29461 attgacctat caagccctaa atctctagcc ttaccagctg gaactacttc aatttcagca
    29521 cttaataaat cctcttcaac tatattatat ttttcattta aaatctttaa tatatttgct
    29581 gttataggtt ctttttcttc atcatctaaa ggaatattcc caattaatat atttaaagct
    29641 tctccactaa ttccttctga taatttcttt tgcatttggt ctccagccaa atgaatcaat
    29701 aggtcagtta cacaaaatac tgggtcatta tcttcttccc caatagatat ctccactttt
    29761 gttccatcat ttaatataac aattccatgt aaagcaagtg gaacagccgt ccactgatat
    29821 ttttttattc ctccataata gtgggtctta aaaaaaccta gattactctc ttgatacaat
    29881 ggatttggtt ttaaatctaa tcttggtgaa tctatatgac ttcctataat cttcatacct
    29941 ttttctatat aatttttacc tattacaaaa agtgctactg ccttctcttt atttataaca
    30001 taaatcttat caccacaact tatgcttcct ttttccatag cttcatctat tgaaatatag
    30061 ccattttctt tagccaattt tataatttct tttactgaaa gtctttcagt ctttgactta
    30121 cttaaaaatt ccatataatt gcgagaatat tgcataattt gttcaatagt attttctttt
    30181 ctttcaaaat cccaagcatt cttaaattcg tactttaagt tcatactttc ctcctactaa
    30241 attctaattt ttattgtttt tcatgtattg ctcatattct tctggtgaaa ctactttgac
    30301 actatctagt cttgacctaa agtgggctct gaaattttca agatattctt tccttaaaat
    30361 ttctctttct ttgatttcat cagcagaaag gattccctct ttatttttct ttgctaattc
    30421 attaatccta tctaatttct ttttgtcaaa cattttttcc tcctaaaaac ttctatttat
    30481 ttaataaaat aatttattgc tttttatatc taacttgttt ttatattcac aaaaaaactc
    30541 atctatatta tcacttatta tagatattcc cttttctagc tcttccatgc ttggacgagc
    30601 tatatttatt ctaaaaaatc tatcatctac tagattgtca aaaaaataag tccctggaag
    30661 cattgacaca cctttgttta acataaaatt tgtaaatgct tgagatgtat atcctcttgg
    30721 taattctaaa aaaaagttta ttcctccaca agattttcta acttttaatt taccactcaa
    30781 tttctcatta atcaagtttt ttgccaattt atacttatca gtatatatct tctctatatg
    30841 actcaaataa tttttccaat caaaattatt catatagtaa tacattgatt tttgaattaa
    30901 acctggtgtt gatatatctg atgaatattt agcccataaa acttttttta acaactcatt
    30961 tggcatttct acaattccta tcctaagacc tggcataagt atttttgaaa agctttttat
    31021 atatactact ctatttttat catcataact tctaattgtt ctattgtcca ttgattcaaa
    31081 aacaaaatca cttataaaat catcttctat tatataaaaa tcatgttctt ctgccaattc
    31141 tattaatttc ttttttttat atgttgagta agatattccg gttggatttt gaaaattagg
    31201 cataatgtat ataagtcttg gttttatttt ttccaacttt aattttaaaa tacctatatc
    31261 aattccatca tctagcatag gtatagatat tatttttgca cctctgcttt taaacacttc
    31321 aattgctcca ttataagaag gttcttccat aaaaacaaca tctgaataac ttataagacc
    31381 tttacaaact atgtctatac cttgttgagc acctgatatt atttgaatat tttccttagt
    31441 agtttttata tttccatcac ttagatactc caccatcttt tctcttaatt cttcagaacc
    31501 aagaccttca tcatattcaa atattgagct accgtcttta gataatgcca tatttacagc
    31561 atttttaaat gaatcaatag gaaacatatc attagatgga ttcccattgt caaaatgaat
    31621 taaatcttct ctttgcacaa taatcttatt ttttctgtta tctataccgc ttttataaga
    31681 atttaaacta gatacaaaag ttccactccc tataatttta tatacatatc cttcgttttc
    31741 taaaagttcg tataccttaa ctatagtggt gttgtttact tgtaaaaaat ttgatagttt
    31801 tcttattgga ggcaattttt cgtgttcaac taactcttta tttattatca attcttttat
    31861 atgattaaat atttgtatat atttagttga ttttttctca tttaaatcta ttctgtattt
    31921 ttccaaattt atcaccgctc attgtattta tattttattg atgcttttac gtatatttga
    31981 tattatttag tatgtatata taataccaaa atttttataa aaaggggaaa tatttatgtt
    32041 ttacgactat cacatgcact caagtttctc tacagacgga aaatctacaa tggaggaaat
    32101 ggtaaaaaaa tctattgaac ttggtttgga ggaaatttgt ttcacagacc atgttgacta
    32161 tgatgtatat gctgatgatt ccttttcaat tgtctacgaa gattatttta agagtttaga
    32221 gactcttcaa aacaaatata aagataaaat atcaataaaa aaaggaatcg aatttggagt
    32281 tcaaactcaa ttaatagata catacaaaaa ggaagcacat caatatccat tggactttat
    32341 aatatgttct atccatgcta tagatacaat ggacttatac cttggtaatt attttaaaga
    32401 taagactcaa cacgaagttt acgaaaatta ctacctttat ttatataata ttgttaagaa
    32461 ttataaagat tactctgtct taggtcattt agatttgatt aaaaggtatg caccttatga
    32521 cactatttta gatgatagat tattttctga tataattgaa gaaaccctta gacaagctat
    32581 ttatgatggc aaaggtatcg agataaatac ctcttgttat agatataatc ttccagacct
    32641 aaccccatct aagtatatcc ttcaaatgta caaagacttg ggaggggaaa tcataactac
    32701 aggttcagat tcacatcata taagtcaagt tgcatgtgag tttgactaca tatattcatt
    32761 actaaaaaat atgggattta aatatgtatc taaatttaat aaattgaaac cagaatttat
    32821 aaaattataa atttttacac ctccttgtga atatatttaa aagataagga ggtgattttt
    32881 tgaaagtacc ttatttagta ctatcaacag ttattttatt tatatccaat tttattgtaa
    32941 gaatatttgg ttttttatat aaaatattct tatcaagagc actaggtgaa acaggtctag
    33001 gtatctatca tatgattttt aactttttga tgatttgctt agctgttaca acaacaggta
    33061 tacctactgc acttagttgt ctagttgcaa aaagaaaagc tttaaatgat agacacaata
    33121 ctaatgcttt atttatatca actttatata tatcattttt cgtagcacta actatatcta
    33181 tagttgcatc tttcaacagt tcttttttat caattaagtt tttaaaagat gctaaattaa
    33241 acctatttat actagctgtt tgtcctgcta tagttataat aactatttca aatgtactaa
    33301 gaggatatta ctatggcata aagaatgtaa aaatccctgc tattggacaa atcattgaac
    33361 aaattggtaa aatattattt gtttttttac ttgttatgta cataaataat aaatctatga
    33421 attgttatat agccctatta ggtatatcaa ttggtgagtt gagcaacatt atatttatgc
    33481 ttatttgctt atggcgagat tcatcatttg acaataggta tattataagc ataaaagatt
    33541 tttataattc ttctatggaa acattaaaaa tgtccattcc cataacttgt aatagaatgt
    33601 caaacatact tttgcaatct ataagttcta tgatgatacc atcaagatta gcattatcag
    33661 gaatgactta tcaacaatct ttaagtatgt atggcgtcat cagtggtatg gtaatgcctt
    33721 ttatattttt accttttact gttggttcag cgcttattgt aaatttaata ccaactattt
    33781 cacaggaaat ggctctaaga aagcgtaaga gtgtaattaa aaaaatcaaa tactcaatac
    33841 ttcttacttt atttgtaggc atactatcat caatattttt ttatttcttt ggaaaagatt
    33901 tatgtatatt agtatttaaa aatacacttg caggagaata tctaaaagct atgtttttag
    33961 ttccactatt tatgtcacta aatcaaactc tatctggcat tttacattca ataagaaaag
    34021 aattagcttc aagtataaat actatcactg gtatgttaat tcaactgata gctttatatg
    34081 tatttcttcc aattccagga cttaatattt atgcatatat atacacaatg acgatagtat
    34141 caatctttac ttgtctatta catactatag tgctttttaa atcactaaaa gctatacact
    34201 agttataaaa acgtgaaggc taccaatgta aattatacat tttggtagcc ttccttagat
    34261 gatttttatt tataatttaa actgtctata tctctaccta tctatctgtg gcaacttggc
    34321 tttccacttt catgattagg tccacattgc gaaaaatcac atactttaga gaaagtatca
    34381 acaagtcttg gttggattgg atttggtgat gctaaaacta ctatttgttc acttattaat
    34441 gtgctaactg tattttgtaa gcaaagtact aaatctatat catttggtat tattaattca
    34501 tagaaaggtt cacaacattc gttacattct aattttaata tagattttcc tatacaatct
    34561 acagttaagt tagtttgata ttcttgtctt aaagtaacag ctcttccgtc agctggcaag
    34621 catattggtg ctgaaagtgt gttaaaagta aattctacat aatcaactct tctcttacat
    34681 ccacctcctg ctctaactgc tgggaaagct aatgcaacga actctgtaca tccacatcta
    34741 cctcttagct ctagtactaa attacgaact gctacagtta atcctctttg tttataagct
    34801 actgattttc cttttccttg tctacagcac tcagcatttc tttctccaca aactgcaaat
    34861 tcaaaagttg tttcacagtt tcttcctact atatctccta atgggtctaa atcgaaatct
    34921 tctaaagttg tcattcctgt atctataact attccatcat tacttaaaca tattttttct
    34981 atagttacac ttgctttacc tgcacttgga actgaaccaa atacttctac tacttctgtt
    35041 tcaaaagtta atggctctcc atttggtcct gttgcatctg taaatgtttg gaattgcata
    35101 gtatcaaata ttctattagc ctctattgca aatggcactg catttgatac agagaagttt
    35161 cttggagata taggattaca gcattctggt ccacatttgt tttcaaaaca ttcatctgaa
    35221 tccatttcac atggttcaca atcacattta caatggtcat ggcatccaca attgtcatca
    35281 catggattag gtttgcatgg gttgcatgga ttaggtttac atgggttaca tggattaggt
    35341 ttacatggat tacatggttt gcaatcatca tgacaatcac atttattatc agtattacat
    35401 tcattatgat gatgtgattt tttaaaccct ctcatatctt ctgaatttat ttcctctgta
    35461 tacacttctt cttcattcat tgtaggcatt ggctgatttg tcattcttct tttatttttt
    35521 ttataatctt gcatttttta ttgccctccc tgtaagcgat tcttattaag tctttggctt
    35581 tttaaagcct aattaatact atgctctttt gagcttaatt gttacttcgc tttttaaatc
    35641 tatcaataat tagtcagatt tagtaaaaaa atgcattttt aataaaaaaa gtactactat
    35701 ttgaaatagt agcacttttt ttgtcccgaa acaccggtgg ctaagtaaat tcacacccta
    35761 ggcactccca ggtgggtgac ttgccagtaa cctttaacgt ccaaactaac taggcatgtt
    35821 ataatttacc agacacggtc tcccgtattt catttgtagg ttgaatttaa aaaagcctta
    35881 tcgtatgttt caggatttca tattattatt atataataaa ttttgaattt tttaaagtgg
    35941 ttttatatag taattgagat aatcttattc tatcattact ttttcttcct tttttcttta
    36001 tgtatttagt taattatcgt tttttcttag ttctggtttt agtatttttt gcattataat
    36061 tgtattcgga ttcttgctca ttaatgttgt atagctcctc atcatatact atatcttcat
    36121 cattttctat attttcatag tctgttttat tatcctcgac taattcatct tcattatcct
    36181 ccatttcatc ttcgtctgtt aactcttcat catcataatc caattctttg tcttcatagt
    36241 ctaattcctc atcttcatta ttcaaccctt cattattgtc ttcatcttca ttattactat
    36301 catcacctct taaaagactt cctaacaatt tgagattatt catactgcca agattgttta
    36361 tatttgccaa agagcttaaa ctaaatccgc cttcaccaaa gtcatctggt aaaaataatt
    36421 ctgcaaataa atctatcttc tttttcattc ctatagcttt ttcaactgaa ttttttcttt
    36481 tttctgggaa tacatctata atttccttta tcatcatatt ctttttatca tcactatcca
    36541 tttttctgaa aaaatccgtt tcatcttcta tatctaccat atctatatta tttaattttt
    36601 ttattcctct aaccaaatct aaaacacttt ctatttttat tagtattttt ctttcatctt
    36661 ttgacatgta ctttttagtt cttgttatta tttccattcc catttctaaa tcttcttcac
    36721 ttaaatccat gagccttaag cctcttttta tgtcatttgc atcaaacctt aaattgctat
    36781 gtgtagactg tgacagttta tcatttgcat tttttttgtt ttttctaact ctattatttc
    36841 cttttgattt atttttatca tcaaatatag agatattacc attactcaat gcaatcactc
    36901 caatcaaaaa tagtaagtta ttcaagacaa ttccccctta tattatataa ttgccctata
    36961 ttaaagtatg tttttcatat tttaatgttc catttttaaa gctcagtgca tatatattat
    37021 ctataagaat ttttatttat attatatatc actcacaatt caccacttca atattatttc
    37081 tatatataaa tagtatgaat ataattcaat atatatttat agaaaggggt ttttttatga
    37141 aaaaattaga tgcagagtca ttagaaaaaa tggctaaaca aaaaaatatt gacaaggata
    37201 aaatagaaaa gatggctgat agttacaaag gaaaatcaga aaatgaactt atggaggaac
    37261 ttataaaaat aggtaaaaac ttagatggaa gagatgaagt agtatcaaaa ttcaaagcgt
    37321 ttttagatga aaatcaacgc aaaaaattgg atactataat ggaaaaaatc tcagatgcag
    37381 aaactcagag agatactaaa ccagctaaaa ctaagaaaac taaacctact aaaggtaagt
    37441 caaattcacc tgcaccaaaa aatacaccaa ctggtcaaca gaaaaaatct aaaggtttat
    37501 ttaagaaaac taaaaagtct aaccctcatg aatagataaa ataaactatt taaataatta
    37561 gacaattatt tttagttaat ctagtattaa atttgatatt attaacaaga aaaatcaggt
    37621 agtgctctaa gcactacctg attgatttta atagttttat ttttttcttt ctatgacact
    37681 ttctctatca acaattctat aaggtaattc tattttctta ttttcaatta attccttatt
    37741 tattaacttt atgaccattc ttattgctac tgctcccata tcgtataaag gttgatatac
    37801 tgtagtaagt ttaggtctat acattttagc tatcttaaca tcattaaatc ctgaaactga
    37861 tatatcttct ggaactttat atccagcatc acatatagca tttatagcac ctatagcagc
    37921 ctcatcacct gttacaaaag ccgcatgagg tattattcca tcatctaata gttctttcat
    37981 actattgtat ccactctcat aatcagttcc accatactta atcaaactct tgtctaattc
    38041 tatattgttg tttgaaagtg ctttctcata accagcaagt ctttccattt ctaaaacagt
    38101 atcatcttta ctagtcatta taaaagctat cttttcatga cctttcttta taagatgctc
    38161 tgtcatatca aaagtagctt cttcgttact agtacttact gtatatatat cataatttct
    38221 agcagtttta cttatatatg tcgctggtat tccacaattt tgtatatagt tgatatgctc
    38281 ttgttcaact atccacgaaa tcataactat accttccact tgttttgctc tcaatagatt
    38341 tatactctta agttcttgct ctttatcaga gtatgtattc gctaaaagaa tatcatagtc
    38401 atacatttta gcaacctctt ctattccatt taacacctca ttaacaaaag aatctgaaac
    38461 ttctggaact attaccccta ttaattgact tttctttgtt actaaacttc tagcaagcgg
    38521 atttggtata tatccagtct cttttataac ctctaaaact ttttgtttga cttcatcagt
    38581 tacaggtttt gaatcattta taactctaga tacagtagat attgacactc ctgcttgttt
    38641 agcaacatct tttatcgtta tattgccttt catcttcatc ctccttattt tactaaattt
    38701 atctaaaact gaaccaaatt tttgaaggtt cctattttaa ctacatttat ataagttttt
    38761 attttaccat aaaagcaagt acatttaaat acattatatt ttatattata ccctatattc
    38821 aacaaattta ataatattca ttcttaattt aaatcatatt aaaaaataaa ataaaaaaaa
    38881 tactggtata aaataccagc attttttatt ataattattt cagtattagc agccgcagcc
    38941 cacttcacca tcaaagaatc caccacttga acaagttatt agtaataata ttaagaaagg
    39001 tatccatgac attatgtcaa tgtctctcca gcaatcttca aaagctaaga ataggaagaa
    39061 tagtactatt atccaccatc ctcctccacc aaaacatcct tcttctccaa aaaatctatc
    39121 taacataaca aagccctcct ttttttaata actgtactct tattctaagc atccaccaca
    39181 gcagcaaagt attaataaaa ctattaggaa tggtatccat gccattatgt caaattctgc
    39241 ccagctatct ccaaaagcta agaataggaa gaatagtact attatccacc atcctcctcc
    39301 accaaaacat ccttcttctc caaagaatct atctaacatt atacgttacc tcctgttgat
    39361 attttgttga gcttttcgct cctaacatat actatgcatt gggtttccat tttgctaatg
    39421 tatttttatt tttttttaaa attttttaat ctgaattaaa aaaggcaaaa ccataacttg
    39481 tataagttac tggttttacc tatttaataa tcaatatttt ttagcaacaa caaccatcat
    39541 cgaagttaaa taataacaac actattaata taacccatat ccatatattg tcatcacata
    39601 ataaatcttc taaaatgtct gtgcataata ataagaatac tataactatt attatcaaca
    39661 ttttattacc aaaaaatcct tcgcatcctc caaagaaacg ttccatattg tcttctaatt
    39721 ttgtcataac taatcctcct tgaatcatta agagactaag ttttgtctct ttaattaaat
    39781 atatgtagta atagacagat aagttacttc taaaaaattt ttattgatta gtttatttat
    39841 ttctttttca ccaagtttat tgtatctatc aacttcatca agataatctt ttttctcctc
    39901 tgatatagta tagattaaac tttctattga ttttattttg tactccttat atctttctat
    39961 caagaaggat gtttttgaat tttctattcc tttctttaca tgcattaacc ttgatatgta
    40021 gtcttctttt tcattagata cataactctc cttttcagct acatcttcaa ggattaaatt
    40081 ggtctccatt acataatcct ctatacgttg catatctaaa gttattgaat tataataaaa
    40141 atttactgct ataagtaaaa acattcctat tgaagttagt aatagtccta catatttcat
    40201 agcatttcct cctaagttat atttgttatc aataaagttt attcatcaaa taatacttta
    40261 ttactacatt tataaaattt tattattttt tatagattcc atattgaaaa attcttgata
    40321 ttatattaaa ctaacattaa aattaattaa aactgcttta ggaggcttaa aatgtttgaa
    40381 aaaataaaag aaataatagc agaacaatta ggggtagaca atttagatga gataactatg
    40441 gaagcatctc taatggatga cttagaagcc gattcattag atgctgttga aattattatg
    40501 gcacttgaag atgaatttgg aatagagata ccagacgagg aagctgagaa ttttaaatgt
    40561 attggtgata tatgtaaata tatcgaagaa aacaaataat atcgttttgt ctacaaatat
    40621 aaatagtctg tgtaaacttc tacataataa attatattag agttcacaca gactagatta
    40681 gattgattca caatatacaa gagataatct cttatattta atttatacaa atatataaat
    40741 tacttttctt cataaaacgt cacattcttt attattatat ctatagtccc atcttgattt
    40801 tgttttacag tatacttcat tgggtcttca aaatcagtta gattcccttt tatatcaaaa
    40861 ccattatccg tttttatact tcttttttta agctttttct caacccattt tttatctata
    40921 ctaaaacttt catcaagacc tttttcttcc atatgctctt taaagctatc ttttaactca
    40981 tcatctttaa ttgaattatc aacaaagtca tttatatcaa cttcatgttt ttcctttagt
    41041 gtataattta atatacttct tacatcttca gcttgcttta tatcattact aagagcattg
    41101 gttatccaat tttcagctgt atttttaaac accttggtct tgtacttatc atcatctatt
    41161 tttttagcat ttaaaaattc tgtcacaaac ttagattctg acccttcttt ttcagcatcc
    41221 ttatctaaaa gccttagatg aaactcatca tttatgccac ttaaactaat caaagctcct
    41281 tgcttttgtc ttaaagtctc tggtatacca atttcatttg gaaccatttg aatattaaat
    41341 ttatcatcta caaattcaat tgaatgagtg tacaactttt tataatctaa tttaagtata
    41401 gctacactct tttcatcttt aatagtgtat aaacaaattg ccaaatcaca agattctagt
    41461 gcagcattaa ttttcataac atcaaacaaa taagatgcaa tttctttaga atttttcaaa
    41521 aaagtacttt catcatatat tatttgttca caacagtttt taattaaatt atcattataa
    41581 tctttaaaaa caccttttct taaatcctca tcttttgcaa ttctttttat ggctttctgg
    41641 aaaaaaccat ctacctcttg attaactttg ccttcaaagt catttaatat tggaacatca
    41701 ctatttttgt ccaaaacatg tattataaat ttatgtatta tcatatatat caccccatta
    41761 tcttaatttc gatattaact cttttatatc ctctggtata tctgctttaa attttaattt
    41821 ttcttttgtt cttggttgtt caaactctag cctgtatgca tgaagtgcct gcctatttat
    41881 aagttcttca tctacacaac catacaactc atcacctatt attccatgac ctatatgagc
    41941 catatgtacc cttatttgat gtgttctacc agtttctaag cttacttcta ataccgttgc
    42001 attttctagt ctttctatta ctttatagtg agtaacagat gattgtcctc tttcatctat
    42061 tatcctcttt attgaatctt ctgtaggtct ataaattggc tcatctatag ttccctcatt
    42121 ttcttttata attccatcaa ctactgttat atactttttt tcaactttat tttcactcat
    42181 tgctgtggat aaagtatgat gtgcataagc attttttgct acaataacta accctgaagt
    42241 attcatatct aacctattta caaatcttat ttttacattt tctttttgat tatctatata
    42301 gtaactaata ccatttgcta ttgttttatc atagtggctt tttgttggat gaactaccat
    42361 aaatggaagt ttattcacca taattatatc aaaatcatca taaattatct gtaaatttaa
    42421 atcttgaggt tcaaaatttg ctttttcctc atttattttt acttctatta aatcaccact
    42481 atacactttt aaactcggct ttttatatac tccatttact aaaacactct tttccctctt
    42541 catttttgat aatgacctaa ctgagaaatt tagcttgtcc aataaaactt cttttaaagt
    42601 catttcttct tcatttgtat acgatataag attatatctt tgattctctt ttttaaacaa
    42661 acttttacct actttctttt atgtcttcct acttttttta tatctttaaa atcatgatat
    42721 tatatatttt acaccatttt tgtttaatag ataataatta tttttaacta taaaaacctg
    42781 taagaataac aaatagttat tcttacaggt taaattcata gttaaatatt taatttgtaa
    42841 atattataat ctaaaactat ctcttaacta ttaaactagt tcccattcca ccacctatac
    42901 aaagtgtagc aagaccagtt ttagcatctc ttctcttcat ttcatataaa agtgtagtaa
    42961 gtattcttgc tcctgagcat cctattggat gtcctatagc tattgctcca ccattaacat
    43021 taactttatt catatctata tttaagtctc ttattacagc tacagattga gcagcaaatg
    43081 cctcattagc ttcaactaaa tctatatctt caatagtcat attagcagct tctaaagctt
    43141 ttttagttgc tggaactggt ccatatccca ttattttagg gtcaacgcca gctgttccat
    43201 aagaaactat agttgcaaga ggctctattc ctagttcttc agctttttct ttagccatta
    43261 ctactagcat agcagcacca tcatttattc ctgatgcatt accagcagta actgttccat
    43321 cttttttaaa tgcaggtctt aatttagcaa gtttctccat tgtagtacca ggcttaatat
    43381 attcatcttt atctactaca gtgtcacctt ttcttccttt tataacaaca ggaactattt
    43441 cttcatcaaa ttttccttca gcttgagctt tttcagcttt attttgactt gcaagagcta
    43501 attcatcttg ttcttctcta gttatattcc attgctctgc tatattttca gcagtaatac
    43561 ccatgtgata gttattaaat atgtctgata atccatcttt tatcattgaa tcaacaaaag
    43621 cagcatcacc cattcttgca ccatatctcg cacttggtac taaataagga gacatactca
    43681 tattttcagc tccaccaact aacattatat cagcatcacc caatgctata agctgagatg
    43741 ccattgaaac agatcttaat ccagaaccac aaactatatt tatagtcata gctggttttt
    43801 ctactggtat tcctgctcct aatgctattt gtcttgctat attttgtcca agacctgctg
    43861 taagtactcc ccctaaaaga gattcatcta tcatatctgg agttatatta gctcttttta
    43921 tagcttcttt agctgctgtt acccctaact ctaccgctga aactgattta aatgctcctc
    43981 caaaacttcc tactgctgtt ctagctgcac tggcaattac tacttctctc atattcatta
    44041 cccccattaa attagatttt tcatatatcc gaaattgttt ttttaaacaa ttttatttaa
    44101 atttttaatt cccaaactct tatttattat aatcatagaa tcctatctta gtttttcttc
    44161 ctaattgatt agctctaacc attttagcta aaagtggatg aggtctatat ttagtatctc
    44221 caaactcagt atataaaaca ttcattatag ctaaaacaac atctaatccg attaaatcac
    44281 ctaatgctag tggtcccatt ggatggtttg ctcctaattt catagcttca tctatttctt
    44341 ctttacttgc aacaccatcc gcatatatac caacagcttc atttatcata ggtataagta
    44401 ttctatttac tacaaatcca ggagattcag atacatctac tggtacttta ttgatactct
    44461 tagataattc aaatactgta tcaaaagtaa cttttgatgt taactgacca cttataactt
    44521 caactaattt catcatagga actggattga agaaatgcat tcctataact ttatctggtc
    44581 tcttagtaga agaagctatt tctgttatag ataatgatga agtatttgtt gccaagatag
    44641 tatcttcttt acataattca tctagtaact taaaaacatc tttctttata ttcatatctt
    44701 ctacagatgc ttctattatt aaatccatat cttttaaatc ttcatagtta gtagttgaac
    44761 taacatgact taatatttct gcttttgtag cctcatccat ttttccctta gtaactaact
    44821 tagttaaatt tttatctaat aaagctaaac atttatctat agcaccttga gttctactct
    44881 ttaaacatac atcatgtcca caacttgcaa aagtttgtac aataccactt cccatagttc
    44941 cactacctat tacagctaat ttcatagtta taacctcctg ttattttagt atttatattc
    45001 tgttaactag attaaaatac tattcaatct agttaacagc aaatatattt ttaattagat
    45061 ttttaccctt ttataaagtt ggcttctctc ttttcaacga aagctgacat tccttctttt
    45121 tggtctttag ttgaaaaaca aagtccaaat aaattagatt ctatatctat tccagtattt
    45181 atatcagttt gagcaccaag ttgtattgct tctttagagt atctaactgc aagttgagca
    45241 tttttagcta ttatcttagc taatttctca acctcttcta ttaaaatttc tggctcaacg
    45301 actctattta ctagccctat tttttcagct tcatcagctt ttataacttg acctgtaaag
    45361 attaattctt ttgcttttgc cattccaacc aatcttgtaa gtctttgagt ccctccatat
    45421 cctggagtta ttccaagagt tacttctggt tgaccaaatt tagctttagc agatgcaatt
    45481 cttatatcac atgccattgc aagttcgcat cctccaccta aagcaaatcc atttacagca
    45541 gctatcacta cttttttact attttctatc tctccaaaag cttttgctcc taatatacta
    45601 aaatctttag cagctacagc atctaaatct ttcatgtatg caatatctgc tccagctaca
    45661 aatgcctttc cttcccctgt caatattaca acatcaatag tttcatcatt attaatatct
    45721 acaaatactt cataaagttc ttctaaagtc tttgaattta ttgcattaag ggctttaggt
    45781 ctattcattt taactgtaca tatatttcca tttacttcaa cagctacatt ctcataaact
    45841 ttaacatcac ttgtactcat aataccctcc ccaagaaatt agttagctaa aacttcacct
    45901 ttttcttttg caacacttaa ctgactgata agttctggaa gtactttatg aacatctcca
    45961 actataccaa catcagcata tttaaatatt ggagcttctg gatttttatt tatagcaact
    46021 ataaactcag catcttccat accagctata tgttgtattg ctccagatat accacatgct
    46081 atataaaggt ctggtcttac agttttacca gtttgaccaa cttgtcttgc tttatctaac
    46141 caacctgcat ctatagtggc acgagaacca gaaacttctc cacctataat ttcagctaat
    46201 tcataaagta tgtctaagtt ttcttttcca cccattccac gtccagcaga aactaatatc
    46261 ttagcatctt ctattttaac ttgtttctta gcttctttta ttacttctac aacttgaact
    46321 aatttatcag catcattaaa ttctaccttg aaacgattaa ttacagcttc tttagtttcg
    46381 tcaggttcat ttttcttcat aactcctggt ctaactgtag acatttgagg tctgaaatct
    46441 ttacaaacta ttgttgccat tatatttcca ccaaaagcag gtcttgtcat taataataat
    46501 tttgtatctt cagctactgc aagacctgta cagtcagcag taagacctgt atgtattcta
    46561 gcagaaactc taggtgctaa atctctacct attgaagttg caccaaataa tacaactata
    46621 gggtcagctg cttttattgc ttcataagct gcttttgtat atggttcagt tgtatacact
    46681 gctaaagctt catcatctac tactattacc tcatctgcac cataatgtgc taatgtatct
    46741 attaaacctt ctaccttact acctaaaagt aatgcagaaa cttttgtatc atagtctttt
    46801 gctatttctg tagcctttcc tagtaattct aaagaaacag tttgaattac attttctctt
    46861 tgttctatta ctactaaaac gttacccatt ctttatcccc ccttaaatta tatgatatac
    46921 ttctctttta atttatctat gataattcca gctgatgttt tcgcatcttc attgtatatt
    46981 gtaccagctg gtttaactga ttttgtaaat gatttaaata cactagttgg agaaccttta
    47041 agacctaaat tagaagggtc aacctctata tcttttacag tccatgtttc tactacatca
    47101 ttttcgaaag catcatatat tcttccaact ttcatgtatc ttggtgtgtt catatcttta
    47161 agagttgtta taaggcatgg cattttaact tttaagtcat ggcaacaatc ttcaaattgt
    47221 ctttttacta atacatattc accttcagtt tttatttctt cagcatatgt tattgatgga
    47281 agatttaaat gttcagctat ttgaggtcca acttgtgcag tatctccatc tatcgcctgt
    47341 cttccagcta ttataatatc aaaatctata ttttttaatg ctcctgctaa tgctgatgaa
    47401 gttgcccaag tatcagcacc tgcaaatgct ctatctgtta ataatattcc tctatctgca
    47461 cccattgcta aagcttcttt taaagccata tctgcttgag gaggtcccat tgttataaca
    47521 gttacatgag cacccatttc ttcttttaat tttatagctt cttctaaacc tgctttatca
    47581 tcagggttta ttatacttgg tactccatct ctaattaaag tacctgtatt aggatctagt
    47641 ttaacttctg ttgtatctgg aacttgtttt atacaaacga ctatattcat aaatccttcc
    47701 tccatatcta ttttaatatc gacctattgt gtatatttta atatttatct attttaataa
    47761 ttttcctgaa ataaccattc tttgaacttc actagttcct tcatatattt cagttatctt
    47821 agcatctctc atcattcttt ctactggata gtcacgagtg tatccatatc ctccatgaag
    47881 ttgtacagct tttgtagtaa cttccatagc tgtttcagcg gcaaataatt ttgccattgc
    47941 tgcttctact ccataaggtt ttcctaagtc tttatttata gctgcttgat atacaaggtg
    48001 tctagccgcc tgtaccttaa cttccatgtc agctaattgg aattgtgtat tttggaattt
    48061 tgataatggt ctaccaaatt gtactctttc ttttacatat ttaacagttt catcaagagc
    48121 accttgtgct aaacctaaag cttgtgcagc tataccaatt ctaccaccat caagagtaga
    48181 cattgctatc ttaaatcctt gaccttcttt tccaagtaaa ttttctttag gtattctgca
    48241 atcctcaaat attaactcac tagtagctga acctcttata cccattttct tttctttaac
    48301 tccaaagcta aatccaggag ttcctttttc aactataaat gctgatatgc ctttgttccc
    48361 cttagattta tcagtcattg ccattactac atatatgtca ccagctattg catttgttat
    48421 aaatattttt gagccattaa gtatgtattc atccccatct aaaacagcag ttgtttgttg
    48481 gccagacgca tctgtaccag cattaggctc agtaagacca aatgctccta atttttctcc
    48541 acttgctagt ggtcttaaga atttttgttt ttgttcttca ttaccatatt gatatatagg
    48601 ccatgaacca agagatgtat gagctgataa tataactcct gtagtaccac aaactctaga
    48661 caattcttca actgccatta tatatcctac agtgtctcca ccttctccac catattcttt
    48721 tggatacggt atacccatca ttcctgcttt tgccatcttt tccactgttt cataaggaaa
    48781 tctttcttct tcatcaagtt ctgttgctaa aggtttaact tcattttcag cgaagcttac
    48841 atataactct ttaagcatct gatatttttt agaatttaaa tccataatag cctcctcagt
    48901 attttataaa tattttattt tcatttatta attaaaatct cggcaacatc tataataaag
    48961 tatttgaata tacgtcgaga aatttataac cttaatcaaa ctacgctcta tatttatatg
    49021 tgattatact aaccaattat gacattaatt taatcacaaa ttgttaattt aaaatcaact
    49081 acacctaaaa aaaatctcgc tttttggaat tttgtgaaat ttcaagttaa aataagattt
    49141 ctttcacatt gattacaaat aaaataattt gtttacaata tttatatcag ggtatactaa
    49201 atagtaaata ttgtgtgtat agctgttttt tagccatgta cagaatattt ttatcgcata
    49261 aaaattattt taataaagct aaataatcat atttttatct gactattttt tatttttaaa
    49321 tatcttacat ttataatagt atcactcaaa ggataatttt tcaataattt ctaattattt
    49381 ttttaacaat atttattaca ttttttccac ttttttgtca tttttttatt gtttcgaaaa
    49441 aaaatcagaa ttttttaact ttattttgga ttattagttt aaattagatt ttcctcttag
    49501 atttataaat atttacacaa aaaactacct ctgcatagat tgtatacaga gatagttttt
    49561 tactaatttc tactatagtt tactatattt ttttaccaat ttttacaaac atctacccat
    49621 ttttcacact tagaaaactt ctccatttct tcacatgtaa gttctattgc agagttacta
    49681 ctaccacatg caggaaatac tgtatcaaat ctcttcattg aatcatctaa gtacactttt
    49741 agagaatttt ttaaaccaaa tggacaaaca ccaccaatag catgtccagt aaactccaag
    49801 acttcttctg gtatcaacat tttagcctta caattaaatt cagccttata cttcttattg
    49861 tcaatcttag catcaccagc tgtcacaatt aaaattgcat catctcctat tttgaatgag
    49921 agtgtcttag ctattcttgc tggaataacc ccagctgctt cagctgcaag ttctacagta
    49981 gcactagatt gttcaaattc taatatactg tcgtccttgc caaattgttt aaaatactct
    50041 cttacattac ttattgacat taaatctccc tcctagtctg aacttttata aatttcatat
    50101 tattcttaat tatatctata attgatttat ttttcaaatt attccataat attagcattc
    50161 catgtatcat tattttttcc aactactaga tacagtcctt ttgccattgg aatattaaca
    50221 acatattctc ccttatattc tacactcata ccagattcgt taaagtacct ataatcaaaa
    50281 tttagtcctt ttggaattac aaaatcaaca taaccttgat tgtttggttt tattccatac
    50341 ttcagagttc tttcagttat agaatttttt tcattaaata aatctatatt cacatatgaa
    50401 gaacacttat ttattccctt tttattttcc acacaatttt caaacttcat tttagcactg
    50461 tttaatttat aactatattt actattatta cttattttaa atcttacaat agtaccaata
    50521 tctctgcttc tcctgctatc aaaatcaaaa acaatttcct ctaaatgatt tatttcataa
    50581 tctttgacac ttaccatatc tctatatact aatttattta tagctatgaa tagtgtaaaa
    50641 atcccaaaaa taaaaaatga aacttttata aactttttca tttctagtct ccttatattt
    50701 tgtgctattg tgctataata ttaatttaaa ttttaataat taaacttatt cttttttgaa
    50761 atcctaatag aaaaatacat tgatattata aaaattatta ttgttaaaaa tgtcatacta
    50821 tataaattta tatacttagc attagccata tcagttacat aatttgaaag atatactcct
    50881 ccaactttac tataattaaa atgctttact tgatatacaa tcatactaat catcttcaaa
    50941 agacttccat atacaaaata aattaaaata cttactataa gaccacaaac tttcattttt
    51001 ccatatccaa acttaaaaat taaaggaagt aaaatcgtca aaattgctaa aaatatattt
    51061 acagaaaaaa atacatcatt taaaaccata tttctaaaga atactaatga tgtcaattta
    51121 ctcattaagg acacaattat tgttacagaa attattagtc ctacacctaa taaatatttt
    51181 gagtaaacta tatcttctat gctaactggc atactcctta taaaaatcct tgaatcattt
    51241 tcataatcac actcaaacgt atttataaat atcagataac taacgacaat acttactgta
    51301 taatatgaaa ttgaattaag gaaaaaataa aacacaaaaa atagcaatat gtactttaaa
    51361 ttacggatac catctgaact aatccctatt attaaatctt ttcttattaa attgaacatc
    51421 atctcacctc atttacataa taatacatta gttcttctat agtaggattt ttgaccttat
    51481 gcttatcaat ttctatcaat aggcttttta ctttcaagct atctccaact ttaacaagtg
    51541 cctcattata atatttatac tctttaacac caactatact ttctttaatt gcttctagct
    51601 ctgctttatt tgccctaatt attttatatt cttgttctat taattctttc ttaacagtaa
    51661 aaatctgatt tcctctattt ataaaaacaa gataatcagc aattttaatt agattatcag
    51721 tattatgagt agaaattatt acactactac ttccattttt aacaaaatct tgtaaatact
    51781 gcattatttc actccttaca attgggtcta tcccagtagt tggttcatcc aaaatcaata
    51841 acttagcatt atgactaagt gcacttgata acataagctt ctcagattga cctttagata
    51901 aatttttaat atgcatattt tcctctatat caaacttatt aaggtattca tcaaatcttt
    51961 ctctatcaaa ctccttatag aataaagaaa ctatttttct aaattcctta acctttaaat
    52021 gtggataaaa ttgtaaagaa tcatatacaa aaccaatatt gtttttaaaa cttattggag
    52081 aattttcaat agtttcatta caaagttcaa tttctccttc atctgcatca ataatcccca
    52141 ttataatttt tattaaagtc gtttttccag aaccatttgg tcctataatt ccaacaatat
    52201 ccccttcctt taactctaag tttatattat ttaacttgaa ttttcctaat ttttttgtga
    52261 tattccttat gctaaacaca tcttcacctc ttacgcattt tttataccca cctatacata
    52321 ttttaacaca ttcactacaa aggtcattta taaatttata aattaagaat tataaaacta
    52381 tgttataata caaagtatga agaggtgaat atatgaaaag aattataact ataaatacaa
    52441 accaattaaa taaatccctt gagactaaag aactcttaac tagaaaactt atcaatgctg
    52501 gctttgaagt gtattcagat atttatccag atacagagct tattatatca attggtggtg
    52561 atggctcttt tttaagaaca gttagagatt tcgattttcc tgagattccc ataatgggaa
    52621 taaatacagg gcatctgggt ttttttcctg atattttacc agataagatt gatagtttta
    52681 ttgaagctta tacaaaaaaa gattatatta ttcaagaaat gtcacttctt aatgcagaag
    52741 tatatacaac tactagtggt tctaatatgc ttgctgtaaa tgaggttgtt ataagaggag
    52801 acaagtctag gactatacac ctaaatttaa gccttgataa taaacatata caaaatttta
    52861 gtggagatgg tatgattatt tctacttcca caggttcaac agcctacaat tattctgctg
    52921 gaggtagtat tgttgatata aatcttgaac ttatgcagat tacaccttta catccgataa
    52981 acaccaatgc atataggtgc tttacatcaa gcataatatg ttcaaatgaa tctgttataa
    53041 aaattgcacc agaatataga tttgaagatt ctgtattgat tgtcgttgat ggagttgagc
    53101 atagatttag acaaatcgaa aacataaaag ttagtatttc tgatgcaaaa ataaaattac
    53161 ttagaatgtc aaactatgag ttttggcata gagtatctga aaaattttta tagactgtac
    53221 ttaggaggat ttttaatatg aaaataggaa taatgagtga tactcatggt agcttacttt
    53281 actttgaaaa agcgttaaat gtattatctg attgtgatgt tttattacat ggtggagatg
    53341 tcttatatca tggacctaga aatgatattc cagaaggata taatcccaaa aaatttattg
    53401 agacattaaa caagttagaa aacattgtga ttgtaaaagg aaactgtgat gcagatgttg
    53461 accagatggt tatagaacac cctattcaga gtccttatgt catgagccaa tttggtgaaa
    53521 ttagaattat attgaatcat ggttatacag aatcagaaga agaaattatt gacaaagcaa
    53581 agaaaatggg tggtgacata ttagttttag gacatactca tgtaaaaaaa ttatacatgg
    53641 atgataattt aatagtaata aatccaggaa gtacttctat cccaaaagat ggaagccatt
    53701 ctgtcgcaat tatagatatc ataaaaactg atgatgagga tgaacttgaa cttgatataa
    53761 acttaattga catcaatact gggaatataa taaatattaa tgattagtaa attttaaata
    53821 aaaacaaagt aaactttaat ttattaaatt cataataaaa aacaagtaaa ccgttttatt
    53881 tctatctcaa ttagaagttt aaggtattgt acttaaattt ctaattgaga tagaatctgg
    53941 tttacttgtt ttattgtata ctatattgaa atataactta gtttacttgt ttttactata
    54001 tattatgcta aagtataact tagtttactt attttattat atattcattt tgatttacac
    54061 aatattactg taatgctaat gcctctgcat atggtttact attcttacct ccaccaactt
    54121 gtacaaattt atcagccttt acttttttta taaaactttt ctgtgcacta cttaaacttc
    54181 cacttgcaag tactacaggt gactttgttt ttgatgctaa tactccagca gcaagaccat
    54241 caactaaatc agcttctcta ccacttccat cttttgcaaa atacatatta ttaaccttac
    54301 ttgatttccc ataaaacttt tctaaaactt ttgcattagt atcttttcta tctgttcctg
    54361 ctagtctttc tgcatttact aactgtcctt caagtttttt acttatactg tactcaccac
    54421 ctactatgta tgtctcagtt gagttttgtg taataaaatt ctttgcctca cttcttatgc
    54481 tatctttacc tgtatatatt atagctgtat tatcacttgc cgctggactt gatattgaag
    54541 ttgcgtctgc cagtccatta taaccattaa ccactgccac ttttgagaca cctaaatcac
    54601 cagtatcatc tagctgttta gtaagaagta aagatgtttc ttctctatct gctccaccaa
    54661 ttctactaac taatactccc atatcttcaa gaaattcttt tacacttgat gaaatagctc
    54721 cttcacttcc aattaaaata acttctgctg gattaagcct ttttatttcc tctgttacat
    54781 tatatggtat actattttta ttggtaagta gtattggtgc atccaattta cttgccaaag
    54841 gagttgaagc caatgcatct ggcaatgaat ttgagttaac taaaaacacc ctttctgaac
    54901 cattattcca tccactttga gatattttta tggctgtatc aaatctatct tcaccaataa
    54961 gtgtttcctc ctttggagat tttctgcctt gaagataatt attcatatct acctctgtat
    55021 tttgattttt actaatagtg tctgcatata cacaacttgg catcataatc ccaagtgaaa
    55081 gtacaaacgc tattattttt ctagatttaa ttttcaatac aattccccct tttaaatgtt
    55141 aatacctatt aatatccata ctttttattg ataccctaaa ctaaaaataa ttatctttta
    55201 taaaaataat cataaaaata gctatatcat ttcagataca atacaaactt atactaagct
    55261 tgacttataa agaatgatat agctattata actttaaatg cttaattttt aaatactttt
    55321 taactaaact ctttcagcat atttttcagc tattcttaca attaatttag tagctttctc
    55381 catagaacta actggtatac actcattttt cccatggaag tttaatcccc cagtaaatat
    55441 atttggacaa ggtaatccat taaaagataa tcttgctcca tcagtaccac ctcttacagg
    55501 aactaaacgt ggttttatat ttaattcctc catagcttct ttagctatat caactataaa
    55561 cataactggc tcaatttttt ctttcatatt gtaataagaa tcttttaaat ccaattccac
    55621 tctattatca tatttcttat ttatttgttc tatattctct accataatag attttctata
    55681 ttcaaatttt tctctacaat ggtctctaat aatataagac atacttacat tctcaacatt
    55741 tccatctata gaatttaaat gccaaaatcc ttcatatcct tctgttgttt ctggtctttc
    55801 atctgctgga aacatttctg atatttcagt agctatatgt atagcattta ccattttatt
    55861 tttagctgaa cctggatgaa catttctacc atgtatagta atagttgcat ttgcagcatt
    55921 aaagttttca cattcaagtt ctccaactat tcctccatct aaagtatatg catattttgc
    55981 tccaaatttt tcaacattaa ataaatcagc acctctacca atttcttcat ctggtgtaaa
    56041 tcctattttt atatctccat gcttaatttc tggatgattt attagatatt ctattgcagt
    56101 cactatctca gctataccag ctttatcatc agagccaagt agagttgttc catcagtgac
    56161 tattaaatcg tcacctttaa attctaccaa ctcaggagaa tcctttacat acgtaataac
    56221 atttaactct tcatttaaaa ctatatcttt tccatcataa ttttctatta ttcttggttt
    56281 aatatctttt ccactaacat cttcaattgt atctaaatgt gatatgaatc ctatagtatc
    56341 cacaccatca gtatttcctt ttaaagtagc cattatataa ctattttcat ctaaactaac
    56401 atcatcaaca cccattgttt ttagctcttc aactatatac ttagctaaaa ccctttgacc
    56461 ttctgaagat ggacagttct cacactgaga attagatgtt gtatcaaatg atacgtattt
    56521 taaaaatctt tctactactt tttgcttcac gtatataccc tctttcttaa taagattaat
    56581 gaaatttttt atttactagt gaataaaatt ttatctttta aaaaatttat caattattat
    56641 aattaaaaaa tatccacctt cttaagtata tcatgcatac ataccaaacc caaacaaaaa
    56701 attactccaa ttattccttt tacttggatt ccaataaaaa tcgctgctag ggtagctaat
    56761 ggatttagtc caacatttac cgatactagt ttaggttcaa gtatttctct aatcaaagac
    56821 aagacaaaaa atatcacagt catgcttata gctataaaat aattttttat aagaaaataa
    56881 taaacaatta ctggaagata tattgttatt atacctagaa atggaattaa atccatcaaa
    56941 gcccccacaa atcctactat tagaccgtat ttcaatccaa ataaagcaaa acttccccat
    57001 attgcaaaaa atgttatagc catgagtatt gtataggctc ttatatatcc aacaagtgaa
    57061 agacctgcct ctttctttac gttttttacc ttctttctga catcaacagt gaacatattg
    57121 taaaaactat tttcaatctt atcaaggtct tttgctataa agtatgtagc aataaacaaa
    57181 gtaattataa atattataat atatggaata gaagtagcca atccaagtat actagtaaga
    57241 agattttttg taatttgcat tacttcacca ctgaatttgt ttactaattg ttctatatca
    57301 aagttggcta tttcttgaaa tgacttcata aaatcattta ttttatttaa aaaatacata
    57361 atagtattac ttatatcttc aggattagca gatatattat ttaaaaatcc cattagttct
    57421 ttcactgaat ttattactat tattgtagta actgtactga ctatagccac tgcaagaaaa
    57481 cttagaacaa gcgtagagat acctttattt atatgaaact tattttctaa cttttgagat
    57541 ataggattaa tcataagtgc tattatacca cctagaaaaa atggagctat gtatggaaaa
    57601 gctttgtaaa taaaaataaa taacaatgta tatatacaaa aaaagatagt gttgttcttt
    57661 aatttatata aaaaattatt ttctagtatg ctcattttca aaatctcctt tcttctaatt
    57721 tatattttta ttttgtccat ttcatactag ttttatttga atataaataa aaagaagtat
    57781 atgaatataa aagccaattt ttatattcat acacttctaa taaatttaag aacattatta
    57841 cattatgtat atattcttat ttttttaatt ctattttttt ctatactttc tatacaaaat
    57901 cttatattat cgacttcaat tacttcattt tcttctggca atctgcttag gtgtccaatt
    57961 ataaaacctc ctatggaatc aaactcctca gattctagat ttacaccaat aagttcatta
    58021 acatcaccaa ttttagtact tccatctacg atatactcat cttcttttat aacttgtatt
    58081 tcatcctctt cttcatcata ttcatcctct atatctccaa caattacttc tactaaatct
    58141 tctatagtta aaagtccaga tgtaccacca tactcatcta caactatagc catctggctt
    58201 ttttcaagtt tcatttcttc aagaagttgt gttatctttt tgaactcata cgtgaaaaaa
    58261 ggctctctca tataattttt aatatcaaat gactctattt cttcatcaga taaaaatata
    58321 atatctttta tgttaagtat tcctacaata tcatctatag tttcttcata cacaggcatt
    58381 ctacttaatt tttcagtttt aaacacctgt attatctcat cataactatc ttccatatct
    58441 atagctacca tatctattct ttgtaccatt gcatttttgg cttgcatatc tccaaattca
    58501 aacacattat ttatgatttc tctctcttcc atttcaagga cgccttcttc atgacttaca
    58561 tttaccattg tttttagttc ttcttcagtt atgaacgatt tagctccttt atttgttata
    58621 ccaaataatt taaatataat atttgtaatt atattaaata tccacactat tggtcttaat
    58681 ataaatataa ttgcttttat aggtttggat actaatagag aaaccttttc tgaattattt
    58741 gctgctattg tttttggagt aatctcacca aatattaata ctaatactgt cataacagct
    58801 gtagcaagtg ccactccttt ttctcccatt aatcctataa acaaagatgt tgatatagat
    58861 gttgctgcta tatttacgac gttatttcca accaaaatag aacttaaaag cttatttgga
    58921 ttttctatta atgaacttac tagcttagct cctttaactc cttcatcttg catatatctg
    58981 attctgattt tactcaatga cattaaagcc gtttcagatg ctgagaaaaa tgcagatcct
    59041 ataagtaata ctattaaaaa tataatctga atcaaattat cgggcgattc caacacttac
    59101 cacactccta attacacgtt tttatattaa tattaattat aaataacttt aatattagtt
    59161 tttattatat cacaattaca tattatttgg aaatattaga taatatattc aattaacata
    59221 tattttctaa ctatattcaa tttttacttt tgaacctatt ccagcctcac tagatgaaac
    59281 taatagctta acaggaactc tatttttaag ttcctcgaca tgacttatta tacccacact
    59341 tagattatta ctatgcaatc tttccaagga ttctataaca atttccaaaa gttcactatc
    59401 taatgaacca aagccttcat ctaaaaagaa aaattctagt ggtgcacttc cctttaattg
    59461 tatttgagat gaaagagcaa gtgctaatga taaagatgtt aaaaatgtct ctccaccaga
    59521 tagagtatca atacttcttc tttctcctcc attaaagtta tctctcatta caaagtttag
    59581 tgttgaatca atttcaagtg catatcttcc ttttgttatt ccatccaatc tttttgatgc
    59641 ttccaacgct atatatttta attggtttgt agaaacatat tctacaaatt tatttccttg
    59701 tacacattta tctaattctt ccaataagtc cacattgtgt tcaactactt taagttgttt
    59761 attaaaatca tttatcttat ctaaagagtc ttttaatgtt attaactgat tttgattggc
    59821 tccaatattt ttggatatat ttcctatttc tactttcaaa ttataaattt catcttttaa
    59881 ctgttcaaag gactccttct ttacacttct tccatttaat ttttcttttg atgattttat
    59941 tttaaatgct aataattttt gttcttcttc atgttccagt atttcttcaa tcaatttttt
    60001 tacttcatca gcatctaata aagctctttt tactgcatat atgctttcaa atttattttc
    60061 tgccaacagt ttatttagtg ttgatttttg gtctttatat tgttccttag cagttttaag
    60121 tctaccatct atattgtgct tttcagcaat gtttttttca tattctaatc tctgctgttc
    60181 caattttttc ttgcttgatt cctcttgctc taatatctta catatatctt cttcaacact
    60241 atgtaaaaga tttttagcta aatcaccttt tgttatgtta ataacttctt tatacttatt
    60301 atctctactc aatttttttt ctaagtataa ttctctagcc tttattaact ctatctctat
    60361 ttcatgtagc ttactttcat tttctcttat tttaatatct agagagtctc tattttttaa
    60421 taaatttgaa tgaccaatac ttagtaattc taattccttt tcattctctc ttatttcttt
    60481 tacttttgac aatagattgg agactttagt aatcgtcttt aatcctatat actcttgttt
    60541 aagtttttta catttatttt ctaaaccttc tatttctatg tttaaatctc ttgttaattt
    60601 cttataatta ttcaagctct ctcttatttt taattcttct ttttcaattt tatttttttc
    60661 ttctttggct aatgttactt tattttcggt actctctttt tctttctccc actcctgaat
    60721 attagacttc aataatgcaa gctttctttg ctcctcatca agttttttta ataattgact
    60781 agatggaata tcccccagtt ttccttttac atcttcaagt tctttagtct tcattttttc
    60841 tatagataaa tacccactta atttagcatt taattcctct atattgtttc ttatacttat
    60901 tttttctttt tctaatttct ccagcttttc tttgacaaag gatatatttt catcatactt
    60961 agttatatta tgattttcat gatgttttga cccacaaact ggacaaggca tattttcttt
    61021 taattcacgt ctaagttcag aggccaaatt taaatacttt agttgttcta attctttttc
    61081 taggtcatct ctattctttc tattattttc aagtttttca tttagtaaat ttacttccct
    61141 ttcagtatta aacttacttt caagtattat ctttaattca tcttgtatac tacttttttt
    61201 gatttcattt tctttagtat tatctgcctt atttcttaac tcagtaacat attcagattt
    61261 taaaagtaaa tctgcactct ttccaggaca ttttttaaga agaacatcca agtgaagaga
    61321 taaattttct aaattacgat ttacatcatt cttatcttta tcaatatatc taacttttaa
    61381 gtttatatct tctgtctctt tgagtatttc ttctagttta tttatctttt gatttttttc
    61441 ttccaataca ttattgtaat ctttctcgta ttcatatgct agaaatattt tttgttttaa
    61501 ttctgcactt atatttactt tattaatctt tccttcaact tcttttatag atttagtaac
    61561 tgcatctttt ctggactctg acatctgctt tactttttct aattcaactt tagttttatt
    61621 taagttaatc ccactttctt ttagctcttt taactcttta tctaataaaa ctaattcttc
    61681 ttctaatttt attgcctgtt gtaatcttat tttttcttca cttaatttag gaacttcttc
    61741 attcttaatt ttgtttattt tttcatatct attttttata acttcaagtt cctgatttaa
    61801 tattactaat ttcttttcta aattacttac tgtaaagcta tcttcatcta tctttttttc
    61861 taaattttga actgaaccta tgtatgggtc taccttttta gcattactat cattttctac
    61921 aagagcaatc ttttctttta tctcaccatt tttcaaatca agctcatttt ttctaagttc
    61981 atttttttct agctttaatt gttcttcata tactgtttta ctttcttcgt aagaattttg
    62041 agctaggtct aaagcattat tcttatcttt ttccaaattt tttaactcta ttaattcttg
    62101 atttatattg ttgtatactt cttctgtaat tccatcatat tgacttaatt ttgattttaa
    62161 atctttcaaa ctttgtaact gtaaattttt tctctttttc actttttctc caagacttct
    62221 tccatatttc tcaagattaa aaattctctc taacatatct cttctatctg cacgtgataa
    62281 tcttagaaat tcattaaatt tgtcttgtgg taaaactact gacctagtaa aatcatttgc
    62341 agttagtcct acaacttgag ctactttttc atttacttcc acaactttat cagccaaaac
    62401 attttgggta ttgtcattta atacttctat taatctggca ttagaggttt ttgtccctgt
    62461 tttacttcta gcaatggttc tatctacaat gtatcttctt ctagtgtttt tacttcctat
    62521 ctcaaactca tacgatataa ttgccttatc gcacacactg tttatatatt cttttgtatt
    62581 tctggatata tttccataca tagcaatagt tattgcatct aagattgtag attttccact
    62641 tccagtagtt ccaaatatcc caaacaaacc tctttcaatt aatttttcaa agtctataac
    62701 ttgtttatca atatagctat tgagccctgt aagttctaat ctaattggtc tcatctgctg
    62761 attcaccttc ttcacttatt atatctagaa ataaatccat aagttctcct tttggctcta
    62821 cacctttact aaaagaataa aattctctaa ataactctgc catagacttt tcttttatat
    62881 ctacactttc ttgttcataa caagatgtta ttattggctt tatttctatt atgtctttta
    62941 atagttcttt cattttttta atttcattct gtgatattac ctcttcagta tttatttcaa
    63001 agtatgacca aatctcttta tcttgatttt gttcacatat atctagtgct tcttctattc
    63061 cattacattt aaatacttct ataggtttat aattattaaa atatacatct tcaattattg
    63121 gtttttcacc agcttttata tccactatgt atgccccttt ggtatatgct ctttcatcct
    63181 tgctatattg aagtggagag ccagaatagt atgcatttaa acgttcagaa gctttttgtt
    63241 gtttgtgtaa atgacctaat gcagtatatt gagccttttc tggcaagtct tttctttcaa
    63301 ccaaaaaact tccacctagt tgtataggtc tttctgattc agtactctct ccacctatta
    63361 caaatatatg tgatacagct atatttatag tatcacttct aaaattttct tctaaacttc
    63421 taaatatatc tcctaccttt tcagaataat ttttttgttt ctcaaaatca tcttcacttg
    63481 aaaaaacttc atttaatctt ttttcacttg gataaggtaa tgtagcaacc acaacatttt
    63541 ctccatttat attcagtttt gtacatccct gagttgcaca tgttatttca aaccctttat
    63601 acttagcttc tatcgtagca cttaatggat aaccatatat aagtatgcct tgttcatgtg
    63661 ctagaggcgt tattgcagaa agtctctctg gattatcatg atttcctgaa attattaata
    63721 cacacctctg accattatta gcaagtcttg atacagtctg ataaaatagt ttttctgctt
    63781 gtgctggagg attagaagta tcatatacat ctccagcaat tataaccata tctatttcat
    63841 ttgattctac tatttttata aattcctcac aaaactttgc ttgttcctca attctagaat
    63901 ggccttctag ggatttccct agatgccaat ctgatgtatg aataaatctc attgtttccc
    63961 cttcctctat cttttaggtt taatttatat ataaactaat aacaaacagc ttcatcaact
    64021 ccaaataaat atatacattt ttcttttacc gacttaccag ttaaagtttc aatgatatct
    64081 gcatacaaat ccaactgttt tttatatttc tctattattt gatttatatt ttcttcattt
    64141 acaaaatctg ttttataatc caccaaaact atttgattat cttcttcaaa ataagcatca
    64201 actatccctc ttaacatgac actttcttca tcataaagtt ttttatcatc attgtttata
    64261 agcttttcgt aaatatatat gtctttcata tttacttgtg cgtaaattga tttttctcta
    64321 tttataagtt cagcattcaa cattcttttt cctatgttag attcaaaaaa cttatatatt
    64381 ttatatggat ttactatact agcctgtttt tctgttatta taccttttga aacaaaacct
    64441 cttatttgtg attttatatc atttacatta cttacatttt ttaagtctaa aacctccata
    64501 actaaatgaa ctattgtacc tctttctgct ccacttattt tatctctttc ttcttcattt
    64561 tgaataaata gaggcttttt taatattact ttttgttcaa atatagtatt tattaactct
    64621 tcttcataat tattttgtat cttttttatt tctgtaacag atatagtagc aggttttctt
    64681 gtgctaaatt catatggata tatatagttt aatttctctt ctatttcatt gtaataatca
    64741 ctgtcaggac tatctacatc tattttttca agaatttttt caatactttc tttctcttca
    64801 ttattcttct tttccactaa aatatcattc ttatcccaca actttccata ccattttgaa
    64861 ttatgttcaa cactaaaaac tgcgtctaat cctacttcat ctaataaatt agataaatcc
    64921 ctatgtttta atacacaagg cattatccaa tctaaaaaat ttttaccttt taatatctca
    64981 tattgtgata ttgtatctga attttcaacc ccatttgacc atctctttat actatcttgt
    65041 atatttctag tagaacctgt tataatcaat ttttctttag cccttgtaaa tgcaacatat
    65101 aaaactctca tctcttccga taaattttct atatttattt tgctttttaa cgcttcttta
    65161 gctatacttg gaaaagaaat ccgtctctca taatctacaa actgaggtcc ataccctaaa
    65221 ttgtgatgat ataaaatact ctttttaaaa tcttgagtat taaaattttt acccattgca
    65281 gagcatataa ctactggaaa ttctagacct ttacttttat gaatactcat tattctaaca
    65341 acatttgcat tctcaccaag tgtttttgca cttcccatat cagaactaga cttttttaat
    65401 ttctctataa aatttacaaa gttgaatatt cctttaagac tagtctcttc aaattgtttt
    65461 gctctctcaa acaatacttt taaatttgct tgtctttgac ttccacctgg taatgctcca
    65521 acataagcat agtatccagt ccttgtgtac aaataccata taaattcatc tgtactcatg
    65581 tacattgact tttctttaaa ttcctttaac ttaattaaaa aatctttact tttgtttatg
    65641 cactcttcac ttggtataaa ttcagactca ttttcattct gagaatcttt aaactcattg
    65701 tactctgctg tactttttaa aacctcataa aaaattttat ctttactctg aactctaata
    65761 tctatcaaat cctctggggt aaatccaaat ataggtgatt ttaatacaga tataagaggt
    65821 atatcttgca ttggattatc tattatttga agtaatgaca atattgtttt tatttcaatc
    65881 gtatcaaaat accccactcc gacatcagca tatgttggta tatccatatt catcaactca
    65941 tctgcaaaca ctggagccca tgcagatgta gctcttaaaa gaataactat atctctaaat
    66001 tccacaggtc tatatccacc tattccttta tcatagacct tttgaagttt tccctcttca
    66061 ttaactttca ttaaatcttt gataatcttt ccaaccattc tagcttcaag ttggatatta
    66121 tctaattttt cttcttcctc tatttcactt tctttgttat tagtcctatc gtctttatcg
    66181 tttataatat catcatcaag tttattatct ttttgtatta gatgtatctc tgttgctcca
    66241 ccaataatag atttctcgtc tgtatccatc tcaaaatttg cacctaaatt tagtctttct
    66301 ttttctgtat attcaatctc tcctatattc tcattcatta tattttcaaa tatataattt
    66361 acagcatcta caacttcttc tctacttcta aagtttttat aaagcattat ttttctatgg
    66421 gaacttccct ttttatcatt atagttatta tatttttgca aaaataattc tggctttgct
    66481 tgtctaaatc tatatatact ctgttttaca tcaccaacca taaatctatt tggagtttct
    66541 gtattggcca cagctttaag caggacttcc tgcactaaat tactatcctg atattcatct
    66601 ataaatattt cataaaactc attcctatat ccaactgcta tatcagaagg tacaatattc
    66661 cctttttcat ctacatcagt aagtatattt aaggcaaaat gctctatatc attaaaatct
    66721 attattcctt tttctctttt cttattacta tattcctctt caaatctcag tacaatatta
    66781 gatattggtt taactatgtt ataaagatat tttatttctt ctctaataga atcattatct
    66841 ttattgaatg tagcacttac tatactttct aaagacttct tagtcttatc tcttatggtt
    66901 tttgcctttt cttttgattc ttttatatac gagggagcat cttttgaaat tctttttact
    66961 ccttttacat aattttcaaa ggacatactt gacatctttt tatatgtctc atcccaagac
    67021 ttattacatg cttgtgaaat gtcgactatt tttttatatt cagcagataa cttttctgca
    67081 aaagtttcta attcttctat tgattccact tcttttaatg ccctttccat gtttagacaa
    67141 agaccattaa tctctatttt tacagtgtct aatatagctc tagcccaaat agactgtgaa
    67201 aaatcaaaat tttcatcaat attaaatctt tctgctgagt caatcaacca ttttttaggt
    67261 tcaggagaag ccattgcaaa tgaatatatt cccaaaataa tatcttgtac ttctttatct
    67321 cctcctcttt ctgcataact ttcaactaaa tttaaaaaac cctcatctct ttcttcatat
    67381 aaatcttcaa atacttcctc tattgcttct tgtttcaaaa tagcacattc agtttggtct
    67441 cctattctga aattagggtc taagcttatt ctatgaaagt ttgattttat aacatccaaa
    67501 cagaatgagt gtattgttgt aatactagat ttatttaata aaacaagttg attttgtaaa
    67561 tgtttatttt ctgggttttc atctaaggct tttcctatgg catctcctat cctctctctc
    67621 atttctgatg cagcagcatt agtaaatgta acaactaata acttatctat atcaattgga
    67681 ttttctctac ttgttatcat ttgaattata cgctcaacta gcacagctgt ttttcctgaa
    67741 ccagcagcag ctgcaaccaa tagattacat tctctacttt ctatgacctc tagttgctct
    67801 tttgtccatt taggagaact cactaattaa catctccttt catcattttt attatttctt
    67861 cattgctctt attatttaaa tttttatatt tattatcttt catagttgaa tcaaattgac
    67921 atatagcaga ataatcacaa aagtcacatg atgttccatc tttatgttta tatggagcta
    67981 tacatatttc tccacttaac atgtcttcac acaaatcctt tatagcatgc tttacatatt
    68041 ttctgagtag tttaaaatcc tcatagctta ttgctgaagt acttttgcct acatttccat
    68101 ctttagtaag acctactggt attactaaag agttttttct ttctccatct attaatgatt
    68161 tatccatctt tttaactatg tgagagtctt ttactacaag ccccttcatt ctaagttctt
    68221 ttaatattgc ttcttgtatt tctacatcat ctttatcttc attaaatttt gctattgggt
    68281 tatttatcct acaatatagt attgcagctg gatttatatt tatatcttta tcctttgcac
    68341 tttcaaggat tgcatctaaa tacacaagta attgtaattg tagaccataa tatatttctg
    68401 ttagacttat ggacttattg cctgacttat aatctactat tcttatatat ttattttctc
    68461 cttcttcaaa ttcatcaact ctatctattt gtccaatcaa acttacttct tgtccatttt
    68521 ctaacactat ttttataggg ggatattttc cattgtcacc aaatttaact tcataatcta
    68581 ttggttcaaa tgaaccttgt tttatttgct gactaattat agttatagca gttgtcaaca
    68641 tattctttaa tctatatgca aggtatttat acctctcaga actatttaat atatatcctg
    68701 gtatctttaa aattatttca tcaactatta tacctatctt taattctata tatttttcat
    68761 caatacctct ccaagataaa ttatcttgta aaagttcttt tgaaaaccta tctaaaatat
    68821 tatgaataaa agttcctaaa tctggtggtg taaatgaata ctctttacgc tttcttgctt
    68881 ttagaccata ttgtataaaa taagcaaatg gacattcagc atatttttca agcctagata
    68941 tactaagaga attactttta tacaatgacc ttattttttt ctcttcaatc ctatgaactt
    69001 gattagtata actaagacct tttataactt tttttgttat agaactatag atttcatctt
    69061 ttaaatagta cctatatata tctaaccaaa tactattaat ctcttcttta ttgtaaccat
    69121 cactgtcata attttttatt acatttataa gttcattaaa tgtaggagat ttaaccgtta
    69181 tttttttaag tatatcttca tcagtgcttt tattttcttc tactaaataa cttttatttt
    69241 ctatatttgg aaatatcttc ttaagtcttg atataattat tgatggtcta agtgttttac
    69301 cctcatggtc cgatattgga tatgttatgg ttaaattctt agatgtcgaa gtaagggctt
    69361 tatatacaag aaactgttct tcaaaacttc ttgtctgact atcaatatcg acttcgattc
    69421 ctttgtttcc aagactttct ctatcattat cacttaatat tccactatcc tttgtaatca
    69481 gaggaaatac tccatcagta gttccaacta gatataaata tttggtatct ggatttttca
    69541 ttctatcaac actacttaca agtacttggt ctatactagg aggaactaac cctaattcat
    69601 attcatcaaa acctaaggtt attaatttta taaatttttc taaagaaatt atttcatcac
    69661 ccataagttc caccatttgg tctaatatat ccaccactat gtcccaaact tgtgaatatt
    69721 gatttgcaac atcaagttct cctttatcct taaaatttac tattaaactt tctatagttt
    69781 ctggcatatt tatatctaat aaaaattcat atatatacct acaaatttct tttactctat
    69841 tttttccttt taatttttct tgtaaagtaa ctattggctc tagtactcta ttttttattt
    69901 catttatttt atcttttaat tccagttcaa attcactttc ttcacctgaa agactttgtg
    69961 ttattctata atcccatttt tcatcaaacc atttcttacc tttaatccca ttagccaaga
    70021 cataattttc taatacgttt atatcatcat tatcaattcc tataagtcca ctttttaaat
    70081 acctaaacat agtttcatac ccatatcgtc tattcttcat ttctaaagct gaaataatta
    70141 aaataactat tggattactt tttgcctctc tcttcttatc tataaagttt ggtatattat
    70201 attcattaaa tatagagtgt actaaaaaat catatctatt taaatctctt gtagcaatag
    70261 ttatatccct atacctgaca ttcttctctc taactaaatg tacaatttct ctggctattt
    70321 cttccacttc tgaatataaa ttgttaaact cttttatctt tatattttca gtttgctttg
    70381 aatatatttt atatggataa gcattataaa atctttctag gtgctgtaat tccttaacct
    70441 tttcaaatct ttttataaca cctgtattta aatttacttg tggcagtatt tttattcctt
    70501 cttcgttgca caattgagtc aattttgaat aagtaaattt cgttctagaa aacatatcag
    70561 ctttactgta cccagtatag ttgacatcat ctacagtcaa agagatatta acacttttag
    70621 atttatttaa tatactcttt atgacattgt actgatttgg tgtaaaacct gtaaattcat
    70681 ctatataaac gcaagctcca tctaaataac tgcttaattc aattttactc gccaatgaag
    70741 ttaacatatc ttgagcatcc acataatttt catgcaattt cccttcaaaa gagttatata
    70801 ttttacttat atcttttaat tttaaactta aagtttcatt atccaattct ccagatatat
    70861 tttctaacat ttcagggctt atattgtatt gtttcatttc tgatatcatc tctgtaattg
    70921 agcttacaaa accagcctga gattttgatt ttgaaaacac atttaattct tcacttacat
    70981 cttcaatagc cctatacacc atcattgctt ttccactaga atttatattt acatctgtta
    71041 atcctcctac ttgtgaaaaa actatattac ttagtgtctt aaagctaagc atcctaactc
    71101 ttaaatactt atctttttct tttcctaaaa atagtttact tactctattt tcagcctcaa
    71161 atgtgtactg ttctggaact aaaagtataa ttgatgtcgt ttcatttttt tgagcttctt
    71221 ttttaatctc atctagtata taggtggatt ttccacttcc acttctacct aatacaaacc
    71281 taagtcccat aaatttccgc ctccaaaata ctataatcta tttctttgcc attacataac
    71341 ttattaaatt cacaatactt gcaatgcttt ttatttctat agcacatacg ttcttcttta
    71401 ttatatacct tttctatatc attgtataga ttgtcttcgt atgtatgttc ttcataatta
    71461 gtgtttttta tcatacttat ataattttga attttatttc tatttgtttc atgtttttct
    71521 tcattatata aaatggtaat tggtaagtca tcaaattcag gctgataata tctcatattt
    71581 atatctttaa catttatgtc catttttaaa actttaggta ttacttcttt tgctaaaaac
    71641 atataaacaa tagtttgtat tctattttct acgtgtttat agtcaatctt tttagactcg
    71701 gttttccaat cccatatatc tatattgtta tttccaatta caactaaatc atactttgcc
    71761 tgtattgtat ctccatttaa ggacatcctt acttcatatt ctggcaaata cgtttttcct
    71821 ttttcaatag gaacaacttt ttttatgttt tccatccact ttaaaaactt tttactattt
    71881 ttattgtcct cattaaaata gattccaaga ggtatattgc taaaatatct ttcacaaatc
    71941 aaatgaaaat ctcttcctgt ttttaaagtt tcataatact ctctactacc aacatcatca
    72001 tttttccaat ttattctatc aatatattta tatttaaatt tcagtggaca tgatttatat
    72061 gtgttaattg aattttgact ataagaaaaa tattttaatt tatcgttcac tcatatcact
    72121 tctctcttaa ctttattttt taatagttac ttactgtaca tattgttcta tcccatttta
    72181 aaagtatttc aagcaatagt ttaataactt attatacatt aatttgacct ttttttaata
    72241 atatgtcgat taagtctgcc taaatatata catggttttt gctcttttct cttattacct
    72301 atatcacttt catctctata agcacttcct gaaagtacta acatctcctt tgctctagta
    72361 attcctacat ataaaagtct aatcttttca tttatagaat caatcttcgt tttatgagca
    72421 taatctgttg aaatacttcc tttcaatata gcttcgactt ctgatttaat aattgccata
    72481 ggatttttat atttttcttt taaataccat ttatcactct ggaacttttg attaatattg
    72541 tcagggaaat tatattcaac aagacctagt aaaaatacac aatcccactc catccccttc
    72601 gacttatggt agttacatat tgttatacta ccaggctctg gctcatatcc atttatctca
    72661 tataccacat ctattatatg attaaatacc ttattcttta catcaaacaa taagttatat
    72721 acatcttcta aatccatatt tatattttct gatgctagat attttatata aaaggatata
    72781 taatcaagta cagctttatc ttctttttct aaattaagtt ttttcccaat aaacaaaact
    72841 aagaggtcta accttattat tggatattct aatatctgct ttataatatt tattcctctt
    72901 ttaaaattta aatatatatc actttcttca ttaaattcta ttaaatcatt atctaactta
    72961 ctaaattcaa aatcataaat aatttcttcg gtagaatagt tttttaattt ttctaaaaaa
    73021 tactttaatt cttcttcatt atcagtatct ataaaaactt tatcaagtac aactattaac
    73081 ttttctatat catcacaatg taaaataaag tcaataatag atgctatgtt atttataact
    73141 ctccttttac tcaaagaatt tggccctaat tcttcaaaga ctagattgtt ctctctaagt
    73201 tctttagcaa cttgtgtcac ttgctcatta aatggaacca agatacctat acttttatct
    73261 ggatatttat tttttatacc cttaacgtat ctagcagttt gttctatttc aaatttccaa
    73321 ctatcatacc actttatatt tatattataa tgttctggat taggattttc tttatatccc
    73381 attccatttg gcacagtttt tatttgcata tcttcaagag catgtctaca ctcttcctgt
    73441 ctaaattcct ttgtaacata ttccacaaga gtgtttgcta aatcaagtat atcttttgaa
    73501 cttcgatttg acatatccat cctataacag aaatctgcac tatttataaa ttctttaaaa
    73561 aacttagggt ctgatgatga aaatgttcct gttatactct gatttatatc tccaacccta
    73621 actaagtttc tattttcact agatataatt tttattatat taccttgaat ttcatttgag
    73681 tcctgacatt cgtcttcaaa tatatatttg tatctagttt gaaatttctt tctaagccct
    73741 tcgtctaagc ttaaagtttt ttctgctaaa attaatatat catcatagtc tagtaatcca
    73801 ttttgcttca atttcctatc atataattta tatattggta aaataatttt aagcattcct
    73861 ttttgtcctc ttgacaatat ttcttcaagt ttatctggag aaattccctt atacttaagt
    73921 tcacttattg catttcccgc aaattcaaaa aaacctcttt cccaagcttc aagagttatt
    73981 tctttccaac gttcatcttt ttgctccttt aaaaaccatc taaaagctct ttctccacct
    74041 tcaaatctaa atctacttat acattcattt aatataatat ttttttgcaa atcgtcagct
    74101 atattaaaat cctcacttaa cattacaact tctggctttt cctttattat ttttacagcc
    74161 aaactgtgta tagtcattac ttcatacgaa ttagtatcat ctattttatg ctcttcaaga
    74221 atctttctta ttctaccttt aaaattattt acagcactat tcatatacgt aagaactaat
    74281 attttaccac ccttgtgctt ctgttcaagt aatagtttag ccactaaatt tgttattata
    74341 aatgtttttc cagcacctgg tactgctggt actgccatag taccattatc gtaatttatt
    74401 attggtattt ggtcttctct ataatttata ttaatcataa ttttcatctc ctttatgtga
    74461 gatttttaaa agtatactat ataatatact ctcttggata taaccattaa tcgcatattc
    74521 acttttatat gcatagattt tctttgcatt taatagcaaa ttatagactg tattgtatag
    74581 ataatatttc ttatagtatt cttccatttc atttgtataa attttttttt cttcaaaaga
    74641 ttttctaagt acaattaaat tacttatatc tttttctatt ttcatattcc aagcattact
    74701 tcctatatct acccatattt gtatacttct atctatattt gatgctatat atgaataagg
    74761 agtagttatt actattttgt ctttggattt caattcttct atatcgacaa cagagtaata
    74821 atcgtttatt gcagtcttta aagcttctat aaatatctct tcttttttca ttccaagtaa
    74881 acttaaactt tcactaaaag actcactttc ggctattatc tttttacata acccaacatt
    74941 ttttttacca tcttttaaac ttagcatttt atctatataa aactgaatta aaaattcatg
    75001 tatttttaaa tcactcttct ttttttcttc aatatactgt aaaatatcac gatactgact
    75061 atgttcatcc atattatcaa tacttaaatg tcttgtttca ttaaaaatct taaatgcttt
    75121 tatcctattt atttctaaaa gtgtttctaa aaagcttaag tattcatcat cctttataaa
    75181 atctaaatat ccatagaaca tgcatgtagc cactaccaat gcatttccat atggataatc
    75241 tacagctttt ttatctttct taatattaaa aacatctata tttttttcta tcaaagaatc
    75301 tctaatttga tactccaata tactattgtt tataggagat ataattgcta tatctcttag
    75361 agatacacct tcttctataa gagatatgat tttttcactt attaagtcta acatttcatt
    75421 gtataattga cttgattgat ttaattttat agttgcaggt aataagtata aatcttctat
    75481 tcctatctcc ttccttttaa tattttcttc tttggactta atttttgata ttatcttctc
    75541 atttatatag tccatatcca cattattaaa tacagaataa tccctactat agtcaaaata
    75601 tatataactt tgtaatgtat tatctaatgc ttctgttata aaatcaactt ctgcattact
    75661 tgagctttca aggctatcta ctattatata tctaacttct ttaaataagc tttttacata
    75721 aaaatcatca ttaagtagat actgattgta taaatataca cttaatgaat tatctaaaat
    75781 ggaatttgat agtaatgaat ttatataata tgatataatt tcatccatct gagtgtatga
    75841 aaatctatat atactatctt tatttttctt tgatgaatat attttttctc caataaaatc
    75901 aaaatcaatt aaattaaatg ctgacttatt tatattagtc attatactct ttgctattga
    75961 tttatttgtt ccagtaatgt cttcaaaata tccttcttgt atcctttttt ccttaacttt
    76021 ttgcactatt aaatattcac ttaaactatt tgatataaag gttggagata cagccttttt
    76081 atttatttca tcacaatttt ctattattag tggccaatat ttcaataatt ctttcttcac
    76141 aaaacttata tatgttgtta ttttcagttc ttcactataa tttaatctta aagacctttc
    76201 atattgtatc tttgtgttgt tgtttggtac taataaaaga atacttcttg aattattctt
    76261 agttttatct aatatttctc tatatttatc taaaggttca tttccacatt cttttaaact
    76321 acttttgtca attaaatatt ccacataatc accttcctca attaaattaa gtgtatttta
    76381 tctaattata tcatattgtg taatttacaa aatacaatca aattat
//
LOCUS       DAFNNB010000003        67257 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0003, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000003 DAFNNB010000000
VERSION     DAFNNB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 67257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67257
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0003"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     regulatory      complement(131..214)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(447..1298)
                     /locus_tag="L3M60_000155"
     CDS             complement(447..1298)
                     /locus_tag="L3M60_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6224864.1"
                     /translation="MFKKKIGVLLASTILVGALAVGCGSNSGGSGNSGNSGDSSKVSV
                     SGSTSIGPLMETIGEKFQEKNKGVSVEVQQIGSSGGIKNAIEGTSEIGMSSRDLKDEE
                     KSAGLKETKIAIDGIALITNTANPIKNLTMDQVKGIYTGKITNWKEVGGNDAPIVIVS
                     REEGSGTRDGFQEIVGFDSEELTKDAQISDGSGNIKTTVEGNENAIGYISFGYVDEKV
                     NSLKINDVEATDANVIANKYPISRPFILVNKEDGLSEKGKALIDYILSDEGQAIVSEK
                     GFIKVNQ"
     gene            complement(1551..3335)
                     /gene="pepF"
                     /locus_tag="L3M60_000156"
     CDS             complement(1551..3335)
                     /gene="pepF"
                     /locus_tag="L3M60_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBN6224865.1"
                     /translation="MGTDRSTIEEKYKWKIDKMYSNKEEIEKDISKVKNLIQEVKEYK
                     GKLSDSKENLYKVLNISENASRIVQNLYVYTHMKQHEDTRINDNQGRATKTEMLSTDL
                     GVATSYIVPEILAIEENKLSEYLKDDKLSFYTKYIKDILRDKPHTLSEREEEILAATS
                     ELSTVPENVYDMLAYADMQFPEIEDEDGNKVKLSHSNYSLFIKSKNSRVRKDAFEGEF
                     STYEKYKNTFASTLYGGIKSEIFYAKTRKYNSAIEASLFSDDVSLDVYNNLISAIDKS
                     IPNLNKYVELKKKFLGLNEIHMYDLYVPLTDKFDMDIPYDKAQDIILEALKPLGEEYL
                     KVIKSAFDEGWIDVYDNEGKKGGAYSWGSYDSHPYILMSYKNDLNSLFTLIHELGHSV
                     HSHYSRTSQPFLYSDYKIFVAEVASTLNELLLINYLLENSKSKDETIYLLNYYLEQFR
                     TTVYRQTMFAEFEKIVHERVENGEPLTADEFTDIYYKLNEKYYGKSAIVDKQIGIEWA
                     RIPHFYSNFYVYKYATGFSAASALSQQILSEGSTAVDRYINFLKSGGSEYPLEQLKSA
                     GVDMTKKESIEEALNVFAELVNKLEKEL"
     gene            complement(3401..4066)
                     /locus_tag="L3M60_000157"
     CDS             complement(3401..4066)
                     /locus_tag="L3M60_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBN6224866.1"
                     /translation="MIDIGSKEIALRLLLALIIGGIIGLEREKIRQFAGFRTHILIAI
                     GSCIVSITSIQLFFDYNLYTNADPARMPAQVLSGIGFLGAGAILKNKGGVKGLTTAAG
                     MWATACIGVAVGYGYYELAILGGLFVMIALFVLRIINTFLFSQEKNVLILKLSNLDSI
                     PFLYDKFEKSQLIIRNMEIECRNEEYWKVNFFVSYAKRIHLQDVMKEINMIEGVINVD
                     YLD"
     gene            complement(4217..4378)
                     /locus_tag="L3M60_000158"
     CDS             complement(4217..4378)
                     /locus_tag="L3M60_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HBN6224867.1"
                     /translation="MANNELEALKNEIEAVREEINTYIEYPEIFQEELVESSKKIDIL
                     INKYMYLSK"
     gene            complement(4456..4965)
                     /locus_tag="L3M60_000159"
     CDS             complement(4456..4965)
                     /locus_tag="L3M60_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HBN6224868.1"
                     /translation="MKTRKMTFLGLMIGYSLALYVLETYIPNPLIVIFPGAKLGLSNI
                     ITLISLMLLGVKDTVIIVTIRVILSSIFAGPLSYLLFSIGGAYLSLLFMYLASKIKGL
                     SIIGISIIGAIGHNIGQLIVASLIIENILAIGYLPFMLMASLVTGMFVGLVSRYTIPK
                     LKHFYSKFY"
     gene            5172..5390
                     /locus_tag="L3M60_000160"
     CDS             5172..5390
                     /locus_tag="L3M60_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBN6224869.1"
                     /translation="MTVYDLKIGEKGFINSIDGDIKLSKRLLALGCINSTEIEVKKVA
                     PLGDPIIIRFRGFDLAIRKSDAKNISLQ"
     gene            5408..7354
                     /gene="feoB"
                     /locus_tag="L3M60_000161"
     CDS             5408..7354
                     /gene="feoB"
                     /locus_tag="L3M60_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBN6224870.1"
                     /translation="MINVALLGNPNVGKTTVFNLLTGSNQYVGNWPGVTIEKKEGFLG
                     KEIKVVDLPGIYAMDTFSNEEKVSKSYLENEDVDVIVNVVDASNLSRNLYLTTQLMQF
                     NKPIVILLNMLDIAESKGVNIDAKKLGEELGVIVVPIIAKKKNGVDRIEEEIKGATQK
                     AFIYQKNFGSETETYKKLESILSRCTKTTYTQKKSISDKIDNIALNPILAYPIFIGVL
                     FLLFKFTFDWVGGPLQEGFASLIEAYISAPVSDMLSNSSPWFKSLIVDGIIGGVGGTL
                     PFFPLIFTLFFGISLFEDSGYMSRTAFLMDKVMRKVGLSGKAFIPIVMGMGCSSPAIM
                     ATRTLESEKDRRVTALIAPLMTCGAKLPIYALFVAIFFPHNAALVTTSLYLVGIVMAI
                     VVALFLNKTAFKTEAEPFILELPEYRIPTIDALIKNTWNKSKGFLIRVVTVMFAMSVV
                     IWGLSSFNVFGYTEDINVSFLATLGHIISPIFKPLGFDDWRTSVAILSGLGAKEIVVN
                     SLNILYGNLTVVLPTIFNGVTAYTFLIFTALYTPCIAALATMKKEYGNKMMFASFAYQ
                     FILAWIMAFIVKSIGGVLFMGNSIVEALIGGIIIALALFILFNKFKSTKNGSVCSGCS
                     ICSGCPSANSCSSKEDSNKKNSTH"
     gene            complement(7513..8568)
                     /locus_tag="L3M60_000162"
     CDS             complement(7513..8568)
                     /locus_tag="L3M60_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBN6224871.1"
                     /translation="MTIQDYMLETPVRMREIISNADSLFNEVKKENLKKIIITGSGTS
                     YHSGVQVQPYLQNLLDIDVVKMYPFMITEDTFKFDNENTLVVGVSQGGSSYSTYNAMK
                     LAEDKGCKIASMAGCKNALIDEISDYILTVNCGEEKSGAKTKGYYCTKLNLMLLGLQI
                     AREKGLISSEKYNDEINKILDAINRFEAVYKLSKQWIERNKKKLVNSKEIRIIGHSDI
                     YGDTLEAALKLLETMRIPVTGYEFEEFIHGIYNAINSDSTIFILDTGKEPRVTKMIDV
                     LSGWTENVFAIGRDVTENDKNLKIDITDNPYYQTFNFIVPIQLICGEIPTLRGIDPSI
                     PKDTKFHMKLGSKKLNK"
     gene            complement(8616..9416)
                     /locus_tag="L3M60_000163"
     CDS             complement(8616..9416)
                     /locus_tag="L3M60_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBN6224872.1"
                     /translation="MNKLTKKELRSMFWRSFALQGAFNYERMQNLGYCYSMLPAIKKL
                     YNKKENQAKAIERHLEIFNTTTVVVPAILGITAAMEEENANNPEFDESSISAVKTALM
                     GPLAGIGDSLFWGTFRIIAAGVGVSLSKEGNIFGPLLFLLLYNIPALALRIFGLKYGY
                     QVGVNSLDRIQREGLMDKIMSMATTVGLFVVGGMVATMLSITTPLKFNLNGAEVILQD
                     ILDKIIPNMLPLTFAFVIYYMLKRKVSVTKLTIGTIVAGIVLHAIGLL"
     gene            complement(9409..10200)
                     /locus_tag="L3M60_000164"
     CDS             complement(9409..10200)
                     /locus_tag="L3M60_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6224873.1"
                     /translation="MLVQATMLAIIAGVGILDGRIFGQSMFDRPIVTGMLVGLVLGDI
                     KSGIMIGAQLELIWMGITGIGAATPPDVVTGGVLGTAFAILSGNGAEVALAVAVPVAV
                     LAQSLGVLVRIINSYFSQRAVFYAKQADFKRVTIMMWLPVILFFLSTSVPTFLAIMIG
                     ADKVTALINAIPKIILDGLGVAGTLLPAVGFALLMDMLFSKKMSVFFFIGFLLASYGG
                     LDITAIALFGVCVAFVLNFYINNKENNQNPQLAINTLIEGEIDFE"
     gene            complement(10235..10657)
                     /locus_tag="L3M60_000165"
     CDS             complement(10235..10657)
                     /locus_tag="L3M60_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HBN6224874.1"
                     /translation="MKKFLIATHGELSRELVDTSKLIIGSLTNIEYFCMTKDKSADDA
                     EKEIRSILLNNKDSEAFVVLTDVFGGSVANICTNLLLQGFKFELLTGVNLPMLLSILL
                     LVEEDTKLIIKSGLEEAKNGIVYVNELLENQNKIKNII"
     gene            complement(10673..11152)
                     /locus_tag="L3M60_000166"
     CDS             complement(10673..11152)
                     /locus_tag="L3M60_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6224875.1"
                     /translation="MVSLMRIDDRLIHGQVAYGWATSLGINVILVANDEAKNDQMKAM
                     SLNLAKPSNVTLYIRGVEESGEIVEKFASSKKSNVLVLVKNTKDALVIAKNSGGVLKE
                     INVGGLRYEEGKKKLTDLVAVDNDDINNFKEISDLGIDIEFRMLPRDKKKQLSNLIK"
     gene            complement(11284..14040)
                     /locus_tag="L3M60_000167"
     CDS             complement(11284..14040)
                     /locus_tag="L3M60_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6224876.1"
                     /translation="MRDEILSFVNECALFNNFDELKKESGIVTEDIVKKFNLSRTQSS
                     RLLNDLVKSDNLIKINSRPVIFLPKSKINDVVGPTKKSIYESLDELKNECKLLKQEIV
                     FNSIIGYDNSLKEVIEQIKTAVLYPNGGLTIMLTGESGVGKTFLAKIIYKYSVSSNVL
                     EKNSPYNVLNCAQYYNNLELLSSLLFGHVKGAYTGANESRAGLIEQSDGGVLFLDEVH
                     RLNSEGQEKLFTFMDTGTFSRIGENGVIRKARVRLIFATTESLSEFLQTFLRRIPINI
                     YIPNIEERGSIEKRKIVEHFIYEESKVLGLPIEITQITLNTILKIKFKGNIGECKNVI
                     KYACGRAYAKNQRRNGNIFVTLQDLPRNLYIENVDIFQFQSGKSKSIVFSPKQFSIEM
                     SNGTNKRSIIYQSFIKCLDYFDRLQKNEITKERFSKKSSNLVTRLMDNLVFSNNDENN
                     NVLMELIISIMQDIFRNLEVNNNIKYDGNNVLAFSSYLYLKDESYQLSDEYKALENKL
                     LIYLKKESYKEYSIISKISTLIMNRLDMYLNNVDTIIMIIFIKSTIIRMKFNQVNAII
                     IAHGYATASSMANVCNRMLGKNIFTAIDMPIDATIADVSKQMSRYLEGDRIKNGLIVL
                     FDMGSLNLIYEDLKSKINSTMLFIDQISTISALEVGNLLMQGKNIEEIAEIMDEETKP
                     NIQLYYPQKNKEYAIISSCFTGIGTAIKIQHLLSESLIGIVDVKIIPYDYDSLVSMGD
                     KEPVFEMYDVLAIVGTANPNVNGVDFILLEDIISGKGEDDVFRIFSGVVENEKIKSIN
                     DSIVKNFSLIRVIDSLTILDSKKIIERIEEGINAIENIQKKKLPNDKKIALYVHLSCM
                     IERLVRQAEIEEYTDMDLLIKNHHSEVKLIKNAFSVLEKSYSVQISISEIGYIYNIIY
                     GLPQ"
     gene            complement(14631..15434)
                     /gene="proC"
                     /locus_tag="L3M60_000168"
     CDS             complement(14631..15434)
                     /gene="proC"
                     /locus_tag="L3M60_000168"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBN6224877.1"
                     /translation="MKTLGFIGSGNMGSAMIGGIVQSGLVKAKDITVSDLSQVALDKV
                     KEEFGVNTTTDSKVAVANSDVVIVALKPNICEKALTPLKELIDANKIIVSIAAGKTIE
                     SLESFIGSDKKIVRVMPNTPALVGEGMSALCKNSNVTDEELNMIKAMFESFGEAEIVS
                     EYLMDTVTGISGSSPAYVFMFIEAMADAAVLAGMPRQQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPAGTTIEAVKVLEEEGFRAAVIKSIVACIDKSKDMSK"
     gene            complement(15759..18128)
                     /locus_tag="L3M60_000169"
     CDS             complement(15759..18128)
                     /locus_tag="L3M60_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBN6224878.1"
                     /translation="MARGTFERTKKLREESINAEPHISIERAVLMTEAYKKYEGSVEI
                     PVLRALSFKHYIENRTLSINDGELIVGEKGDSPNGAPTYPEICCHTMEDLEVMHNRDI
                     INFSVSEEARKIHKEEIIPFWKKRQTRDKIINAMTPEWLAAYEAGMFTEFMEQRAPGH
                     TVCGDTIYKKGFLDLKKDIEARLKELDFLNDLDAYNKKADLEAMAIACDAMVILGKRY
                     AEKARQMAEEETDEAKKKDLLLIAETCDVVPAHKPETYHQAIQMYWFVHIGVTTELNI
                     WDAFTPGRLDQHLNPFYERDVENGILDRDRAQELLECLWVKFNNQPAPPKVGITLKES
                     STYTDFANINTGGINPDGQDGVNEVSYIILDVMDEMKLIQPSSNVQISKKTPQKFLKR
                     ACEISRKGWGQPAFYNTEAIVQELMEAGKTIEDARLGGTSGCVETGCFGKEAYVLTGY
                     MNIPKILELTLNNGYDPISKKQIGIETGDPRNFKSYEELFEAFKKQLHYMIDIKIEGN
                     AVIENICAKHMPCPLMSTIVDDCIEKGKDYQRGGARYNTRYIQGVGIGTITDSLTAIK
                     YNVFDKKKFDMDTLLKALDANFEGYEAILNLVSNKTPKYGNDDDYADEIMQEIFNAYY
                     NEVTGRPTVCGGEYRVDMLPTTCHIYFGEIMGASPNGRLCAKPVSEGISPEKGGDTNG
                     PTAVIKSCAKMDHIKTGGTLLNQRFAPSVVQGEKGLDNMANLVRAYFNMDGHHIQFNV
                     FDKNVLLEAQKNPQDYKDLIVRVAGYSDHFNNLSRTLQDEIIGRTEQTF"
     gene            complement(18133..19041)
                     /locus_tag="L3M60_000170"
     CDS             complement(18133..19041)
                     /locus_tag="L3M60_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBN6224879.1"
                     /translation="MNPLVINLQKCSIHDGPGIRSTVFFKGCPLECVWCHNPESQTYT
                     KQVLYNEERCSRCEACINICPHKAIYKGETKICLDQDKCEFCETCLDYCVNNAREIVG
                     QEYSVRDLVKEIDKDRIFYEESGGGVTLSGGEVMAQDMDFICGVINMCKSKGIHVAID
                     TCGYAKSENYERVAKYADLFLYDIKLIDEEKHIKFTGKSNDLILKNVKVLSELGANIN
                     IRIPLIVGVNVDDENLEVKKMIEFLKPLNIQAVSLLPYHNIGKHKYDKIYKKYEGEEL
                     QRPSEEKLEEIKRLFEASNFNTEIGG"
     gene            complement(19386..20465)
                     /locus_tag="L3M60_000171"
     CDS             complement(19386..20465)
                     /locus_tag="L3M60_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBN6224880.1"
                     /translation="MSRRKKGISLVILVAIISSILSSFLTIILVKDNLVGKSTGSSTP
                     IIVNDDGKSQNIYQAVAEKATPSVVGITTTSVDTNNMFAIPTETQGVGTGIIVDSNGY
                     ILTNSHVISDGQATSVNVLFNDGSTTSGKVVWFDQQLDLAIVKVDKTGLTPAEFADSD
                     KVKVGDISIAIGNPLGLDFQKTVTQGIISGLDRTIQTEKTTMTGLLQTDASINAGNSG
                     GPLLNQKGQVIGINTAKASKAEGLGFAIPINTAKSIVEEVIKTGKYEKVTLGIKGTDV
                     SNYEAATGTKLSTDKGVYVAEVISGSSAEKAGVKVGDIITKVGDTDITGMNDLNKKLY
                     TLSKGASTKITVNRGGKAVTINVNF"
     gene            complement(20620..21969)
                     /locus_tag="L3M60_000172"
     CDS             complement(20620..21969)
                     /locus_tag="L3M60_000172"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBN6224881.1"
                     /translation="MRKINFDYSKATEFFCQNEIDVMQPYVDVAHDMLHNKTGLGNTF
                     LGWIDLPKNYDKEEFDRIKKSAEKIKSDSDFLLVIGIGGSYLGSRAAIDMVSHSFRNR
                     LKKEQRNAPEVYFVGHNISSTYIMDLLDIIEGKDISVNVISKSGTTTEPALAFRIFKD
                     YLEKKYGKEEARKRIYATTDSSKGALRQLATEEGYETFVIPDDVGGRFSVLTAVGLLP
                     IAAAGLDIDAMMKGANDAREAFQNPDLKNNDSYRYAVARTILHRKGKDVELLVNYEPQ
                     LHYVSEWWKQLYGESEGKENKGLFPASVDFSTDLHSMGQYIQDGKRLLFETVLNVENC
                     KRNITINSEEVDLDGLNYLAGKTVDFVNHKAFEGTLLAHTDGKVPNLVINIPQLDEYN
                     FGYLVYFFEKACGISGYLLGVNPFDQPGVEAYKKNMFALLGKPGYEKEKEELEKRLK"
     gene            complement(22233..23861)
                     /locus_tag="L3M60_000173"
     CDS             complement(22233..23861)
                     /locus_tag="L3M60_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBN6224882.1"
                     /translation="MNIIISLMGGLGLFLYGMNLMGEGLQKSAGTKLKKIIKLLTSNL
                     FMGVLVGTGVTAVIQSSSATTVMVVGFVNAGIMTLKQAIGVIMGANIGTTVTAQLVSF
                     DLTGMAPIALGVGIILYLFGNNPRIKNIAEILIGFGILFTGMDFMKMAVEPLRDYQGF
                     TDLLVTFGRYPILGLLLGFGITAIIQSSSASMGMLVALAAEGLVPLSAALPILYGQNI
                     GTCVTSLLSSIGANKNARRAAMMHLIFNVLGTVIFLIFLNKPIVSMVTSWDPSNVARQ
                     IANTHTLFNIISVLILLPFTNFIIKLAIKLVPDRAGDIDEDETKTIKYIDDRMIETPS
                     IALANTIKEALRMGEKAKESLNASMEALVEHSTEKIDKTYRRERLINDLQKAILNYLL
                     KLSKAPLDDDSREVVDTLFNTVNDIERIGDHAKNIAELSQVAIDSNISFSEEGQSELD
                     VMYNRVVSAYTYALESMRTDNVDLACKVIKIEEQVDIMEKSCRANHMYRLNNNLCSIE
                     NGVIYLDVISNLERISDHAVNIAQQVIAKRLGND"
     gene            complement(23984..25588)
                     /locus_tag="L3M60_000174"
     CDS             complement(23984..25588)
                     /locus_tag="L3M60_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224883.1"
                     /translation="MLRISNVKLGIDKDISLLKDIVLKKLRIKEKNLIKYSIYKESID
                     ARKKGKMEFVYSVDVEVKDENKILKSKIKDVTKIKEIRYINVPMGDKKLKNNPLVIGS
                     GPAGLFAGLLLAQMGYEPIILERGLDVDSRTKDISDFWTTRKLKNNSNVQFGEGGAGT
                     FSDGKLTTRIKDIRCRKVLEELVQFGAPDEILYSHKPHVGTDILKNVVKNIRNEIISL
                     GGQVRFNSKVTDIVVSNDSIQSVVINDTEKIDTDTIILAVGHSARDTYEMLYSRGVKI
                     VQKPFAIGARVEHPQDLINKAQYKDFYNHPRLGAADYRLIEHVSNGRTAYTFCMCPGG
                     SVIASTSNDFEIVTNGMSEHARDKINANSAFLVNVTPEDFKSDNPLAGVHFQQKYEKL
                     AFELGGKNYNAPVQLVGDFINNKTSLNLGNVEPSYRPGYTLTNLSECLPSFVTETMRE
                     AFISLDKKLNGFAMYDAVLTGVETRSSSPIRIVRDENTLESVSVRNLYPCGEGAGYAG
                     GIVTAAVDGIKCAEKIIQKYTNNTKI"
     gene            complement(25636..25953)
                     /locus_tag="L3M60_000175"
     CDS             complement(25636..25953)
                     /locus_tag="L3M60_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224884.1"
                     /translation="MDYIGIENITPYENTYEFSVYEYDDEITLGNEKLYVCELRVVLI
                     KVNSLYVERLHKSVEAMVLVKNLKKDLDKTLVVNKIKNFVLDEIWVENLVKENIEVIF
                     VES"
     gene            complement(25958..26323)
                     /locus_tag="L3M60_000176"
     CDS             complement(25958..26323)
                     /locus_tag="L3M60_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224885.1"
                     /translation="MSLSLGNAIAVSTNFIKSNPAVPVYNGEEIYAVIYHIEIGYNNG
                     VSPDTKQYIIIDYKEFDLSMDKKNELINIAKEYCKSKGIVENENLEINLLDNEEAENF
                     LEKVFNNMKVIRGLITKKG"
     gene            complement(26404..27441)
                     /locus_tag="L3M60_000177"
     CDS             complement(26404..27441)
                     /locus_tag="L3M60_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBN6224886.1"
                     /translation="MLGRNELCPCGSGKKYKKCCLNKDIVSERACRKTELSQKQYTDL
                     YSKLYDYSRQDKFKEECEKAKEMFYIMQNDSVNEKFERFFNTYLIQDHIMENKKVMTV
                     GFLEENGANLSQSEVSILKSLFESYVSIYEVKEISAGKIVLKDCLSGNELCTEDVKLL
                     RSFKIGSCMIARIVDIEGTNILIDITISISNEVKDIILKDIMNLFNQYQDVYKDMKSF
                     LIYHTHILYKYIQQLLDPSIAEYLKKERDKNDTKVNSENELAEEECKVLDTLKENVEK
                     ENIDACIEFWNEYKKNHENIKGAENGWAAAVEYYVKKEAGQPITQVQISKKYDISSST
                     LGKRYKDLKIS"
     gene            complement(27694..28278)
                     /locus_tag="L3M60_000178"
     CDS             complement(27694..28278)
                     /locus_tag="L3M60_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224887.1"
                     /translation="MRLNNREKNLLKILLILVIMVASDIVVNYLEENLTSLKNEKQNI
                     INKINNQDNYQIKGNKHIDDKYIESVELNENEELKNNFNNSFLDKKTNKDIVLLVENE
                     LKNIVNIEYINQESSLDNHNKKQVNLEIKVNGELENVLKFEDVMKKLRLNNKLEKIEI
                     NKLKVNNFDAQNILSEYKQEKDNIVECKMTVRVT"
     gene            complement(28293..28736)
                     /locus_tag="L3M60_000179"
     CDS             complement(28293..28736)
                     /locus_tag="L3M60_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224888.1"
                     /translation="MKVDKTINFLNGFKKTKSCNKVYKIIIVLILTCILFKSFMYVKS
                     INELEDYISQNKLVDKSKHTISTTDKTREKFSKIKLNEIKKIYETIGINSITRIWIDK
                     NKLEIEGVCSDLDVLDNLRKIKYIKYYYIDSIVRDGEYYRFRIKS"
     gene            complement(28737..29609)
                     /locus_tag="L3M60_000180"
     CDS             complement(28737..29609)
                     /locus_tag="L3M60_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HBN6224889.1"
                     /translation="MATIKKILNLEINDDYIRLVFLRKSHKKVFIDKSIIKEINFNIK
                     NDSNSIEKIKYIEDVVRFVKEEIDKSKISYKNLYFNIQIQDIVIRNVEVLDTKKKRDI
                     LSMIEFEITQYIPINISNYSIKYKVINSINDKLNIQVILFPKSIIQLIKNISENLNMN
                     PKTININFDILQKLINLNLIENFSEEGVFIECKKSQLIINITKDKKIEETYLLSKERK
                     SYEFIKRLLRDFKYIYYYGIENCFIEEYFKYTDKSIEVNPLRLKDKVKVENNFDTDLE
                     DNSINYISSIGMII"
     gene            complement(29663..30013)
                     /locus_tag="L3M60_000181"
     CDS             complement(29663..30013)
                     /locus_tag="L3M60_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBN6224890.1"
                     /translation="MINKKRKGFTLVEMIVVVTILGVISSIALVKYSKVQESAKLNAD
                     YTNAANIVTAASMAINDDENIIDSLSVEALKEKGYLNTVPVPQSTSGKFELVINDNGT
                     DISVNINSKQFYPK"
     gene            complement(30067..31152)
                     /locus_tag="L3M60_000182"
     CDS             complement(30067..31152)
                     /locus_tag="L3M60_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBN6224891.1"
                     /translation="MRLSKNIFCNLEKFYLVLNRISDKEIRVICKEIGILMESGCEIT
                     KIFEIIESQSSKKVKNLLNIVSNHIQKGNSIAESFQITGIFSKFFISMIKAGETSGNL
                     DIIMSDLSNYYDKEYKLKIKIITISIYPIILIILSILSMLFIFVFVIPNFQVVFTSNG
                     IEPPLITRVLMGISTVVTNNLAYLIFSFILFSIGSIYFFITNDNIKKLTNSLKFKVPF
                     VKRINQLVATTRFSRALSILISSGIQIVEAIDMSASVIDNEFIYERLKISNNYIKKGN
                     SIGKSLNLANIFPKLFISMTNTGEESGKLDKSLDVINKFYENELNNKIEQIMKFIEPV
                     LIVFMGLIIGIFVLAMVVPMFDAITSF"
     gene            complement(31323..32702)
                     /locus_tag="L3M60_000183"
     CDS             complement(31323..32702)
                     /locus_tag="L3M60_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBN6224892.1"
                     /translation="MIKIILEENSLPMLIPEKLAKKYNVFPVEFQDDNLVVEIEDEDI
                     YALQDLRLATGKEIILKKEKKELISEKIEKYYNSFELDEDYAKKLFENLLEKAIKENA
                     SDIHIEPFKEHLIVRMRVDGELKEVSKFLVDVYPSLSTVVKLKASMDITEKRLPQDGR
                     VDIKLGGNLIDIRVSSIPTVYGEKIVLRILNRDTFLKDKLELGFSEEAIKSINRIINK
                     RAGILLVTGPTGSGKTTTVYSLLNDLKGINKNIMTIENPVEYKMDGISQIQVNSKVGL
                     SFDVGLRAILRQDPDIIMVGEIRDAETAKIAVRAAITGHLVISTLHTNDAVSSIARLL
                     EMQIPPYLLNASLIGVISQKLVRKVCNHCSHEIMIKDDFSGDVNTKVAVGCEKCNDKG
                     YFGRTAIYEILEINDDIKTCIRNMEDSSTIKDVAQNNGMITFEDSCKRLINEKITTLE
                     ECLVVNEMI"
     gene            complement(32695..33375)
                     /locus_tag="L3M60_000184"
     CDS             complement(32695..33375)
                     /locus_tag="L3M60_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBN6224893.1"
                     /translation="MKKQYVFIILASTILGILVGTIFRDYSNEETVYTSKDKSIRKEI
                     KMTRKSIKELTKEKGKLDKEIDSMKKNQKNSEKISQIETLRDSLSYSDMKGSGIILSI
                     DAIDEKTGNIANSIDYNKILVKVINELKLNGGKYIEINGQRINQYSEVVLAGSHININ
                     GVPVAQPYEIKCIGDKEKLSNYVDKGSKYLDSIQTNYPMKISIKTEENISMKKMNIPN
                     KLKYIKGD"
     gene            complement(33978..35057)
                     /locus_tag="L3M60_000185"
     CDS             complement(33978..35057)
                     /locus_tag="L3M60_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6224894.1"
                     /translation="MEGKVKKAFPGGNTSQGSYSFFDYIIPENVKRVFCLKGGPGVGK
                     SSLMKKIYKEFLHRGYDIDLYHCPSDPSSLDGLVIKKLGVVLMDATAPHTMDPKLPGA
                     LDEIINLGDYWNTEELEKNRQEISVYDKDISNSFKRAFKFLKSAEPIFRDIEEKYSDC
                     MDYGKVNLVTEEFIKRLFDGVKSTGNLKREKHLFGSAITPVGCLDYTENILKDVNKVY
                     YLAGEIGTGKTTLLNKVYNKAIEKGLNVEVYHYPLIPEKIETILLTDLDIGITISTNF
                     KEEDTIDLNQFLNREKLLKYEEDIKFDEKVLDDLICWAVLNLKRAKSKHDIIESYYSP
                     NMRFDEVGKLRDNLVKRILKYEENL"
     regulatory      35343..35458
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35633..37003
                     /locus_tag="L3M60_000186"
     CDS             35633..37003
                     /locus_tag="L3M60_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6224895.1"
                     /translation="MKQKWIVLIIICIGVFMSTLDGSILNIANPTIAADFKINMSQIQ
                     WVVTAYMLVVTATMLFFGKLGDKVGSNRLYTLGFFIFTIGSFLCSMSNNLSTLISSRI
                     FQAVGASILMATGLGIVSNAFPTNEKGKAIGITGAVVGIGNMSGPVIGGIILEHFGWP
                     SIFIINIPIGIIAVFLGIKFLPKPVLDEQSKSFDIPGLLLFASCTTLILLAMNEKGNT
                     RLYLGIIALIIFLLLALREVKFEQSFIDLPLFKNRNFTVGNIIGVACYFPQMAVSFLL
                     PFYLEQLKNLSPMMAGSVMTVHPLIMVLIAPIAGSLSDKHGAKNILTASFSFMTISLV
                     GMALLKADSPLYLLIVCLVIFGLGLGAFSSPNNSSILADVPPQKQGYGGSFLATVRNL
                     SFALGTAFFSSFFAQSLTYNQKFKSHTSAYVIASNKSYWIAASVCFIGLILTVFFMRK
                     TDKSIS"
     gene            complement(37267..37878)
                     /locus_tag="L3M60_000187"
     CDS             complement(37267..37878)
                     /locus_tag="L3M60_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBN6224896.1"
                     /translation="MKIRSSEITMSAVNKSQYPAEGIPEIALAGRSNVGKSSIINTLL
                     NRRNFARTSQTPGKTRTINFYLINNEFYFVDLPGYGYAKIAKSEKEKWGGIMERYLES
                     RQELCSIFLLVDIRHEPTADDKLMYEWIKHFGYNCVVIATKADKISRGQYQKHISIIR
                     KKLQMESSEKVIPVSSLKKTGVEELWEEIVNQYNQHGYEITVD"
     gene            complement(37868..40231)
                     /gene="lon"
                     /locus_tag="L3M60_000188"
     CDS             complement(37868..40231)
                     /gene="lon"
                     /locus_tag="L3M60_000188"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBN6224897.1"
                     /translation="MEQNYTKIDHELPLIPLRGLAIFPYMILNFDIGREISLKALDQA
                     MMDEELIFLTSQKEAEVDEPGEEDFYHVGTICKVKQMIKLPGDTVRVLVEGVSRGKVK
                     KIEQEDGYFRAVIEEIVFDSDNLDSETEVEIEAFVRNVFDAFEEYINIGNRVSPEILI
                     SLADIEDVDRFIDTIAANIYLKSSQKQEILEEFDIRKRLELIYSILLEEIDILKIEKK
                     ITLRVKKQMNKVQKEYYLREQLKAIQKELGEEEDINSEADEYREKLKKIKAPKTTKEK
                     IEKEIDKFSKISSMSPDVSVSRNYLDTIFSLPWNKETKDKLDITKAKDILDEDHYGLE
                     KVKERILEYLAIRTLAKSLKGPIICLVGPPGTGKTSIVKSIARALNRKFVRISLGGVR
                     DEAEIRGHRRTYVGSIPGRIINGVKEAQTKNPVFLFDEIDKMAADYKGDPASAMLEVL
                     DPEQNKDFVDHYLEIPFDLSKILFVTTANSLGNIPRPLLDRMEVIEVSGYIEEEKLNI
                     AKKYLLPKQIKEHALKENFIKIDDETLRSIINHYTREAGVRTLERTIGKICRKVAKKY
                     VEDPTLEEVVISKSDLEAYLGKDMFKYQLAEVNPQIGLVNGLAWTAVGGVTLEVEVNV
                     LKGKGEIVLTGKLGDVMKESAKTGISYIRSIVDKFDIDPDFYKTNDIHIHIPEGAVPK
                     DGPSAGITMALAVISALTKRPVPGNIAMTGEITLRGRVLAVGGVKEKLLAAHRAGITK
                     VLIPKECEADLDEIPENVKEKMEFVLVEHMDEVLEQALLKSGENNEN"
     gene            40411..40923
                     /locus_tag="L3M60_000189"
     CDS             40411..40923
                     /locus_tag="L3M60_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBN6224898.1"
                     /translation="MLKLFLTFLKIGAFTFGGGYAMVPLIQDELVNKQKLLEQEEFID
                     YLSIAQSYPGVLAVNISVLLGYKLYGIPGVLICTLGSALPSFCIVLVLSYLYFKNSSS
                     KILDGFFKGVAPVVIALILYSFTSMFKKLPKSKTNILLLIIAVLSVGVFNISPIYLII
                     GGGVYSLCQK"
     gene            40911..41438
                     /locus_tag="L3M60_000190"
     CDS             40911..41438
                     /locus_tag="L3M60_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBN6224899.1"
                     /translation="MSEIILLFLIFLKIGAFSFGGGYAMLPFIQQEFISKYHFITNQE
                     FLDLLAISQSTPGPVAINSATFVGFKTAGFFGSLAATVGVTIFALIGLTIISKLFSKF
                     KNNKKVENLLTVLRPITLGFILSAAFSSAKEVNWDLYGIIAFIASFYLLYKGKIGSIA
                     IVFVFGILGILLTFL"
     gene            complement(41507..42757)
                     /gene="clpX"
                     /locus_tag="L3M60_000191"
     CDS             complement(41507..42757)
                     /gene="clpX"
                     /locus_tag="L3M60_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HBN6224900.1"
                     /translation="MSKYEEKRQLKCSFCGKNQDQVRRLIAGPNVYICDECVELCDEI
                     IQEEIEDTIDEDTTSLPKPKEMMEILNDYVIGQEKAKKALSVAVYNHYKRIYSKKSSS
                     KDIEIQKSNILLLGPTGSGKTLLAQTLARTLNVPFAMADATSLTEAGYVGEDVENILL
                     KLIQAADFDIEKAERGIIYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVAN
                     VPPQGGRKHPHQEFLKIDTTNVLFILGGAFDGLEKIIQKRGGDKTLGFGAKIESKKEL
                     DLGKLYEKVLPEDLLKYGIIPEFIGRIPVLATLELLDEDALMQILQEPKNALVKQYKK
                     LLELDDVELEFEEGALRAIAKKAIERNTGARGLRSIVESVMMETMFEVPSRDNIKKVI
                     VTEKSVNEDSVNPIIVLKDQEESA"
     gene            complement(42776..43360)
                     /gene="clpP"
                     /locus_tag="L3M60_000192"
     CDS             complement(42776..43360)
                     /gene="clpP"
                     /locus_tag="L3M60_000192"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBN6224901.1"
                     /translation="MALVPVVVEQTGRGERSYDIFSRLLKDRIIFLGDQVNDATAGLI
                     VAQLLFLEAEDPDKDIHLYINSPGGSITSGMAIYDTMQYIKPDVSTICIGMAASMGAF
                     LLAAGAKGKRLALPNSEIMIHQPLGGAQGQATDIEIHAKRILKIKETLNEILSERTGQ
                     PLEKIKMDTERDNFMSALEAKEYGLIDEVFTKRP"
     gene            complement(43510..44796)
                     /locus_tag="L3M60_000193"
     CDS             complement(43510..44796)
                     /locus_tag="L3M60_000193"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBN6224902.1"
                     /translation="MKAELIKKEGNKVTLKITVDNNKFEAAVNKAYNKSRNKYNIPGF
                     RKGKAPRIVIETQYGKGIFYNDAIEILFPEVYPEAIKELDIDPIDNPDLDIEEISKDN
                     GLVMVLNVEVKPEFELGNYKGIEIAKVENTVSDENVDAKLQEMVEKNARLVSVEDKAL
                     EDGDTAIIDFEGFENGVAFDGGKGENYNLVIGSNTFIPGFEEQLVGKKAGEEVEVNVT
                     FPEEYHSQDLAGKPVVFNVKINDVKVKELSALDDEFAKDTSEFDSLDELKADVRAKLE
                     EEAKNRADAETRNSVVEKVAENTEIEIPEVMIQHQIDNMLNELNYQLQYQGFGLQQLL
                     EMTGRTMEELREERKEDAKKLVKSSLVLEAITKAEGIEATEEEFKAELEKMASAYNME
                     VEKIEASLRDADKEDIKGQIKIRKTIDLLVDNATIA"
     gene            complement(44990..45460)
                     /locus_tag="L3M60_000194"
     CDS             complement(44990..45460)
                     /locus_tag="L3M60_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBN6224903.1"
                     /translation="MKIGIVSDTHMMIKNMEKTIPYLKECDLIIHAGDNFTDSRYIHS
                     MTNVGIIAVKGNCDFDAVEEEVVFEVANKTIFLCHGDKYGVKYGTHMLEKKATEIDAD
                     IVIFGHTHTPFREIKDGVLYINPGSTSLPRGVSYKSFVIMDIEEDNINIEEIRI"
     gene            complement(45460..46809)
                     /gene="rph"
                     /locus_tag="L3M60_000195"
     CDS             complement(45460..46809)
                     /gene="rph"
                     /locus_tag="L3M60_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBN6224904.1"
                     /translation="MTRSYDRSNEQIRPVKITRDYTKYAEGSVLIEMGETKVICTASV
                     EEKVPPFLRNSGTGWINAEYSMLPRATQQRKIRDASRGKIDGRTQEIQRLIGRAIRSV
                     VDLNKLGERTIWVDCDVIQADGGTRTASITGAFVAVAEAIYKLYKAKTVKEMPITNFV
                     SAISVGIVNGTHMLDLCYEEDSKAQVDMNIIMTDKCEFVEVQGTGEEKPFSRTDLNLL
                     LELGEKGNKELIRLQKEALGEIADEILGMDYGNEVVIATNNAHKLEEIGEILKDFEYK
                     VYSLKDVDLAGIDIVEDGKTFEHNALIKARTIAKKTKLIAISDDSGLEVDALGKKPGV
                     YSARYAGEHATDEENRKKLLKAMQNVPMSKRNARFVSAIAVVFPDGKEFVVRGICEGM
                     IGFEEKGKNGFGYDSLFIVKGYDKTFGEIPSVIKNSISHRANALKLMKQEFIKRVVK"
     gene            complement(46968..48992)
                     /gene="ligA"
                     /locus_tag="L3M60_000196"
     CDS             complement(46968..48992)
                     /gene="ligA"
                     /locus_tag="L3M60_000196"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBN6224905.1"
                     /translation="MSIKDEINKLIETINYHNERYYNQDSPEISDYEYDKLMKELISL
                     EEEHPELKRIDSPTNRVGGKPLDKFNQIVHKTPMLSLSNAFSEQDLRDFDKRVQEYVG
                     ENVEYVVEFKIDGLSVGLTYNNGEFEKGATRGDGVVGEDISQNLMTVKSIPLKINDKK
                     ELIVRGEVYISKDNFRKVNEQQEEQEQPLFANPRNLAAGSLRQLDPKLTAKRPLDIFV
                     FNMENIEDYDLESHSDSLEYLEKLGFSVSQSYKVCKSIDEVIEHIEYWTDNRGNLEYE
                     IDGMVIKVNNIKQRDEMGYTAKSPRWAIAYKFPAEKKKTKIVDIIVEVGRTGTITPSA
                     ILEPVRLAGTTVSRATLHNEDYIREKDIKINDTVLVQKAGDIIPQVLEVIKEERTGDE
                     IDFKMPEKCPVCSEPTIRLEGEAAVKCINMSCPAQIRRGIIHFVSRDAMNIDGLGESI
                     ITLLLNEKIIQDVSDLYYIKKEDVVELERMGEKSADNLINSIEKSKENDLWRLINGLG
                     IKFIGVKAAKILAYNFKDLDDVMSATSEQLEELEEFGSIMANSVVEFFKEDKNMNVIN
                     KLKNVGVNTKSLDNTGEKVEKIFEGMKIVLTGTLPTLKRNDAKDMIEKRGGKATSSVS
                     KSTTFVLAGEEAGSKLAKANDLGIKVIDEERFLELLKLSSKDEVKAILES"
     gene            complement(49179..50117)
                     /locus_tag="L3M60_000197"
     CDS             complement(49179..50117)
                     /locus_tag="L3M60_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBN6224906.1"
                     /translation="MKVLFTINYGKEKFDKIRDLGYDVIHYSEDIIENNEDVNTADVL
                     VTYNPFERLDISKMKNLQYIQTTSVGIDQLPKEEVLKRNIKIANNRGGYSVPIGESIV
                     LYILEIYKNSAKFFKQQQNKEWKMDFSVSELYGQRVGFLGTGTISSEAAKRLKAFGLE
                     IWGVNTAGSSKKYFDKCFATEDMNIVFEECDIVVVAMPSTKSTDGIVNKDMFKLMKDG
                     SVFINVGRGNTVNQKDLEECIEKFRGVALDVFESEPLNKDNKLWECENVIVTPHNSWV
                     SDKNKERTFNMVYNNLKHYILENKPVDNVVDISRGY"
     gene            complement(50219..50641)
                     /locus_tag="L3M60_000198"
     CDS             complement(50219..50641)
                     /locus_tag="L3M60_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3783 domain-containing protein"
                     /protein_id="HBN6224907.1"
                     /translation="MSFEKINNNNLDKIHNRKCIMLVNFNKKELSLIKNVSNFAGIRD
                     HIILSDKNGDNIIRDILDGNISSECENGIKSKSVIFNNAPSTQVNAFLEGLKKVRINR
                     PFTAMVTETSIDWTLNNLIENLVQERNAMSLGKTIEHK"
     gene            50829..51407
                     /locus_tag="L3M60_000199"
                     /pseudo
     CDS             50829..51407
                     /locus_tag="L3M60_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            51734..52006
                     /locus_tag="L3M60_000200"
     CDS             51734..52006
                     /locus_tag="L3M60_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBN6224908.1"
                     /translation="MQYLLIIIIGLLLFIEVDKLKKQVKSQQKQIDTLCEQTGNNQLA
                     TYHISDEDKELILHLKNSGKDVEAVKKVREITAMELEEAKQYVDSL"
     gene            52041..52259
                     /locus_tag="L3M60_000201"
     CDS             52041..52259
                     /locus_tag="L3M60_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBN6224909.1"
                     /translation="MDNGGVQMDKRHWFLLGYLIIPVCFFVAVIVVGLLRSHSLIEIY
                     NDGLGITALYYLLLSLFVYIRWQYFSDK"
     gene            52280..52465
                     /locus_tag="L3M60_000202"
     CDS             52280..52465
                     /locus_tag="L3M60_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HBN6224910.1"
                     /translation="MYIIYTVLAAIVGMALVVTVDMGITKIFDKKSIFQKNYIKMPLS
                     YFIGIFVFLLIVRLIFS"
     gene            complement(52531..52839)
                     /locus_tag="L3M60_000203"
     CDS             complement(52531..52839)
                     /locus_tag="L3M60_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006426016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224911.1"
                     /translation="MKKKLLFATIILVLLAGILYYISLPDYLVFNSMSFSNGANRDTE
                     LQVIVYQYWNTDEVVAEIEAEHNQINGTPTTLIINLYHSKWSFHNGYEPFYSTIINYD
                     "
     gene            52953..53168
                     /locus_tag="L3M60_000204"
     CDS             52953..53168
                     /locus_tag="L3M60_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004845965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6224912.1"
                     /translation="MKYGHLELHIEELLKEKGISKNTICKELDIPRSNFNRYCRNEFQ
                     RLDAQLICKLCSYLDCGIEELIEYVKE"
     gene            complement(53361..54044)
                     /locus_tag="L3M60_000205"
     CDS             complement(53361..54044)
                     /locus_tag="L3M60_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6224913.1"
                     /translation="MNTIAERIKFAMKAKNKKQVDIVKDTGISKGAFSSYLSGQYNPK
                     ADKMELIADSLDVDLRWLYGQNVPMEHTSQNDNALQYVFYNNSCSEYLLDDLDDIYIA
                     MMTQYAALIPRFYVLVNRAGNAMHILPLFLKEDSSQFYECPSDFFYSDRHTIFTRDFE
                     SIHMVLTTATIYYYGIDTKTYEPKVTMLSYSQADDCFHINNGIQGGHIKAFEKELMKE
                     ALYLKHNDQ"
     gene            54293..54622
                     /locus_tag="L3M60_000206"
     CDS             54293..54622
                     /locus_tag="L3M60_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004613680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224914.1"
                     /translation="MQITLTKEQVKEAFGELRFMGADNCYRYDKDSKKKTEEVEALKL
                     HLGSMKLGNSVDIRLEATELPKIEPYAVVELEESVYAPYVQRGNFPVLVEKITCKGIR
                     PVPNKNM"
     gene            54681..55790
                     /locus_tag="L3M60_000207"
     CDS             54681..55790
                     /locus_tag="L3M60_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBN6224915.1"
                     /translation="MATPLQIGGMGNLEERSGSFLPELPYTNRGVIRQKEALSALIDW
                     CQITIKEVPLEAVIEDVLRIPLELMTVTGYEKGIAGHEVVAIFDNIKVLKPTGNAQYQ
                     GFQILMSGKGCRNYENFLQLNEETWFDFLNRVCQYHINVPRIDLAIDDRKPYLSIPDL
                     IVRTKEGLLSTKLREIDFHDSGELKEEVFQSKGGSLYLGSSASNLRLVFYEKGYEQNK
                     KYGTELDENWNRYELRFRQEMAVSVVQELLRYRDVAGLAMEVLNSKMRFLEKPTDSMT
                     TRKRLYPTYQAWAELMKDIGKVKLTMQPQKKSLQKVWKWLEKYVAPSLKLFAEVGKIE
                     QRDYIGNLVKNGEMNIAQKQLYDDYIKARKLDERG"
     gene            55810..55992
                     /locus_tag="L3M60_000208"
     CDS             55810..55992
                     /locus_tag="L3M60_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6224916.1"
                     /translation="MNLKEVSEYLGIGETKTRELLRDRNCPFSMKIGYRWYANKKILD
                     KWVQEQSEKKGGNYSI"
     gene            56078..57352
                     /locus_tag="L3M60_000209"
     CDS             56078..57352
                     /locus_tag="L3M60_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6224917.1"
                     /translation="MGKSLKGKELGAGLYQRKDGKYCGRFYDRFGIRRYVYGKTISET
                     KEKLNTAMYEDKMEMNVADNTVTLDEWYQKWLEIYKYKVIAPSTRVQYESIYKKHIQT
                     DFGHMKLKDIQPLQIRGKLVNLDKKGFGYETQNKVRILLLDMFNKAVADNVAMKNPVR
                     NIKVVRDEEKEPRVLSTEEQSAFFDTCKGTFYDNLFNVAISTGLRPGELYALEAEDLD
                     FEKKVIHITKSLSYQKFEGDTGKTFHMGKTKTKSSYRDVPMNRRCEIALKKQLLQKRV
                     VSGRNGAKTLKGFENLIFTTKYDTPLNAQIYADAIKVIVEQINLMREELEQFETFSGH
                     CFRHTFATRCFEAGIQPKTVQKYLGHATLQMTMDLYTHVLENHLSDEMDKLDSRLEMI
                     ELSSDDLAEQRFEREYRKQNEVLSSDKLIRIG"
     gene            57461..58171
                     /locus_tag="L3M60_000210"
                     /pseudo
     CDS             57461..58171
                     /locus_tag="L3M60_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(58247..58762)
                     /locus_tag="L3M60_000211"
     CDS             complement(58247..58762)
                     /locus_tag="L3M60_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6224918.1"
                     /translation="MNYFVIADPDKCIGCRTCMIGCVVAHSDEDIFYQNPDEINFNPK
                     LSVIKTKEVSAPIQCRHCEDAPCAKACPNGGIVRVGNTIKINEENCIGCKTCMLACPI
                     GAIDIVTLKDVDEGKLCFRERMTANKCDFCIESPEGPACVNVCPTKAFTIVKDEDIDS
                     KVKNKRKLAIL"
     gene            complement(58762..60138)
                     /locus_tag="L3M60_000212"
     CDS             complement(58762..60138)
                     /locus_tag="L3M60_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBN6224919.1"
                     /translation="MIINIDKDLCTGCRECSKVCPVNAIEGEEGKPQEINLDRCVMCG
                     QCVQTCKSYASVIDEGFEFLQEKKQEREIPESINEPIFAAYNVCDIDKVKKALSDPNV
                     FTMVQCAPAVRVALGEDFGYYLGYLGAGKMAAALRALNFDRVYDTNFAADLTIMEEGS
                     ELIKRVTEGGTLPMFTSCCPAWVKFMEKNYPKLTNHLSSCKSPQQMGGAIFKTYGAEI
                     NNVDASKIFSVSIMPCTCKKYECDREEMDSSGYRDVDVVLTTRELAYLIKDMGIDFKN
                     LREEKFDSPLGSYSGAGTIFGVTGGVMEAAIRTGYELITGESIPDVEVKQVRGENGFR
                     KSTIKIGDLDLRVGVVSGLKNVIPVLEDLEKGKLDVDFIEVMTCPVGCVSGGGQPKIL
                     LEEYKELAYENRTKATYVHDEHLAIRKSHENPDIKKLYDEYLVEPLGEKSHHLLHTTY
                     CIGKEVAK"
     gene            complement(60163..60714)
                     /locus_tag="L3M60_000213"
     CDS             complement(60163..60714)
                     /locus_tag="L3M60_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6224920.1"
                     /translation="MKNKIGSFVIADPNKCTGCRACEVACFTMHNQHNNVGYTVGTVD
                     IPVIPRLYLVKGDNFCMPIQCRHCEDAPCLNSCPQKAIIKENNIMSVNEEKCIGCKTC
                     LLACPFGAIDLLPQYQDGKEVEQVILDENKKIAYKCDLCKDSEKIACISACPQEALKL
                     VTPIDDKKAKNRQAALSLLLTNK"
     gene            complement(60935..61768)
                     /gene="fdhD"
                     /locus_tag="L3M60_000214"
     CDS             complement(60935..61768)
                     /gene="fdhD"
                     /locus_tag="L3M60_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBN6224921.1"
                     /translation="MNNIYSKTKLQQNSKDKVFENSYSLVEHKYLNSFNVEVSKINEH
                     EVSIDNEEIIAEYPLSFILNNKYINTFLCTPSNLEELIVGFLKTKGYIENKKSLLKFE
                     INESERFAKVTIHEKQKNQNEQIIFLNSLDYITCAPVESNIKINYDTIYSIMDRNLNS
                     SELFKNTGGVHSVSIFNFDKRIVTCEDVARHNAMDKAIGFCVLNDIALKDKIVAVSGR
                     ISLEMILKAAMTQIPIVVSKSAPTNLSIELSKKLNITLIGFVRGRRMNIYANPQRVIK
                     K"
     gene            complement(61817..63961)
                     /gene="fdhF"
                     /locus_tag="L3M60_000215"
     CDS             complement(61817..63961)
                     /gene="fdhF"
                     /locus_tag="L3M60_000215"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(63545..63547),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBN6224922.1"
                     /translation="MEKKILTVCPYCGAGCQLYLVVKDNKILRAEPANGRTNQESLCL
                     KGRYGWDFLNDPKILTPRLKKPMIRKQGVLEEVEWDEAINYAASRLNEIKEKYGPDSI
                     MGTGSARGPGNEANYIMQKFMRATIGTNNVDHCARVUHAPSVAGLAYSLGSGAMSNSI
                     PEIENADVLFIFGYNGADSHPIVANRIVKAKKNGAKLIVTDPRVTESARIADIHLPIK
                     GGTNMVLVNAFGNVLIEEGLYNKEFVQNHTQGFDEYKEIVKPYTAKYAEKITGIPEEL
                     IRKAMREYAKGKKAMILYGMGVCQFGQAVDVVKGLASIALLTGNFGRESVGIGPVRGQ
                     NNVQGACDMGALPNVYPGYQNVTDDKIREKFEKAWGVKLSPNNGYSLTQVPDLVLKEK
                     KLKAYYIFGEDPVQSDPDASEVREALDELEFVIVQDIFMNKTALHADVILPATSWGEH
                     EGVYTCADRGFQLMRKAIEPQGDVKPDWQIISEISTAMGYPMNYKNTKEIWDELRQLC
                     PSFLGATYEKIEAQGCVQWPCKSESMEDKGTMYLYEGQKFSTPNGKGNLFAAEWRPPM
                     EVEDDEYPFSLCTVREVGHYSVRTMTGNCRTLSSLEDEPGRVQINSNDAKKLGIEDDE
                     LVRISSRRGSVITRATVTDRVKEGATYMTYQWWVGACNELTIANLDPISKTPEYKYCA
                     VKLEKLEDQELAEKCVREEYKSLKDKMTATNI"
     gene            complement(64212..65270)
                     /locus_tag="L3M60_000216"
     CDS             complement(64212..65270)
                     /locus_tag="L3M60_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBN6224923.1"
                     /translation="MDRLKSIRKSLLQYTFLFLLIGITIYLVFKTLDIKMLSNVMVMV
                     DKKFLFIGALAIMCHIMLEGVVMKIIIESTHKVNIRFIGFKLAIMGFYYNLITPFASG
                     SQPVQIYVLKKCKMPLSKASAVVTNKSIIFQIVVTFYCTILVLMNFAMLEKQMKMIMP
                     LVFLGIAINSFVLIMIILVILNPQKIKYFMRITIKHLSKIKFLKFLDNKIESIESFID
                     DYSKAINFFAKNKKVLISTIIVTFIQLSAYFSVSFWIYKAFNLQGYSYIYILTLQAFL
                     YMAISPIPTPGNVGANELAFFTIFKSVFPQPLMGYAVFLYGGFMYYLILIGSGIFTVI
                     THYRMKNRIGRNIVLSEN"
     gene            complement(65481..66140)
                     /gene="cprR"
                     /locus_tag="L3M60_000217"
     CDS             complement(65481..66140)
                     /gene="cprR"
                     /locus_tag="L3M60_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CprR"
                     /protein_id="HBN6224924.1"
                     /translation="MSKILVIDDELDVLKLIENVLKRDGHEVKVISDIENAMDIEFNS
                     FNLIILDVMMPKMDGFEVCKAIRNKVDCPILFLTAKNMESDIMYGLGIGADDYITKPF
                     GVGELRARITAHLRRESREKKNLLTISNVQFNFLGKEVLIDGKKINFTKSEYLICEFL
                     AKNKGQVFSKERIYESVYGFDGESDISTITEHVKNIRSKLKQYDIYTIETVWGIGYKW
                     V"
     gene            66450..67079
                     /locus_tag="L3M60_000218"
     CDS             66450..67079
                     /locus_tag="L3M60_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HBN6224925.1"
                     /translation="MDEKIRSSNYNVPFDYARKKFKELDPHTISNLSNISFNNDSNKF
                     ILNFFDRQYIIDYPSGEVWNDDGSICKNFELKILIIRYLTNAKGIPRTNKYVTFKEIP
                     GGNVYYPNFLNRTLSRLSEVFTCQIDKYKLVMENMGAEKVDMGDLAYKFTYMGNTSMI
                     FILWFGDDEFPPNSNILFDSNIIYYFNAEDLAVVPDTAIDAILNKINQL"
BASE COUNT        21153 a        11681 c         7932 g        26491 t
ORIGIN      
        1 aacaaatggc tactttaaaa gccaaaattt cacctatcaa taaaaatctt aacgacgaac
       61 actctttttt tcaaattttt caacattttt gttaatttta aaacaaaaaa agactatgca
      121 atataaaaat tatattcata gccggttgca ccactaactc catatatctc tagatgccca
      181 ataataagat atacttctca gtttctaaaa tatttttatt aaaaatcttt tatatatttt
      241 cataaatttc ttttttattc taacattgta atattatttt gtcaatattt ccatattttt
      301 agatataaaa tactaaaata tatattttat ctaaagatat ctttattttt acattcaaga
      361 atagacaata ccctgcaagt taaagcatca tttttatgag cttatctcac agggtattat
      421 acatctactt tatattataa tttatgttat tgattaactt taatgaatcc tttttctgaa
      481 acaattgctt gtccttcatc acttaatata taatctatta aagctttacc tttttctgat
      541 aaaccgtctt ctttattaac taatataaat ggtcttgata ttggatattt attagctatt
      601 acgtttgcat cagttgcttc aacatcattt atttttaagc tatttacttt ctcatcaaca
      661 taaccaaatg atatatatcc tatcgcattt tcgtttcctt caactgtagt ttttatattc
      721 cctgaaccat cacttatttg tgcatcttta gttaattctt ctgagtcaaa acctacaatt
      781 tcttggaatc catctctagt tcctgaacct tcttctcttg atactattac tattggtgca
      841 tcatttcctc ctacttcttt ccaattagtt atctttcctg tatatatacc tttaacttgg
      901 tccattgtta gattttttat tgggttagca gtattagtta tcaatgctat accatctatt
      961 gctattttag tttcctttaa tccagcactt ttttcttcat cttttaaatc tcttgaactc
     1021 attcctattt cagatgttcc ttctatagca ttttttatac ctccagaaga accaatttgt
     1081 tgtacttcta cactaacacc tttatttttt tcctgaaact tttctccgat agtttccata
     1141 agaggaccta ttgaagttga acctgaaaca gaaactttac ttgaatctcc tgaatttcct
     1201 gaatttcctg aacctcctga gttagaacca caccctactg ctaaagctcc aactaaaatt
     1261 gtactagcta aaagaactcc tattttcttt ttaaacattt tacttacccc cgcttatatt
     1321 ttaatttttt gttgtcattt ttctatatct taataataca gattttttgt taaatgaata
     1381 ttagagcagg gttaagtaaa tgtaaatatt gttaaataca tgtaaaaact taattttata
     1441 taatttattc ctaattagat taacttttta aagttagttt taaatagata taaacataat
     1501 acaactcata ttattataat tcttcattga aactatcaac ctattacatt ctataactct
     1561 ttttctagtt tattaactaa ttcagcaaac acattcaatg cttcctctat agattctttt
     1621 ttcgtcatat ctacaccagc agattttaat tgttctagtg gatattctga ccctccactt
     1681 tttaagaagt ttatatatct atctactgca gtacttcctt ctgaaagtat ctgttgactt
     1741 aaagcacttg cagcagaaaa tcctgtggca tacttatata catagaagtt tgaataaaaa
     1801 tgagggattc tagcccattc tattcctatt tgtttatcta ctattgcaga ttttccgtaa
     1861 tacttttcgt ttaatttgta gtatatgtca gtaaattcat cagcagtaag tggttctcca
     1921 ttctctactc tttcatgtac aattttctca aattcagcaa acatagtttg tctataaact
     1981 gtagttctaa attgttctaa ataataattt aataagtata tagtttcatc cttagattta
     2041 gaattttcta ataaataatt tataagaagt aattcattta atgtagatgc aacctctgca
     2101 acaaatatct tatagtctga atataaaaac ggttgacttg ttcttgaata atgactgtga
     2161 acagagtgcc ctaattcatg aattaaagtg aatagagaat taaggtcatt tttatagctc
     2221 attaatatat aaggatgtga atcataactt ccccaagaat aagctcctcc ttttttacct
     2281 tcattatcat atacatcaat ccagccttca tcaaaagcac tttttataac cttcaagtat
     2341 tcttctccta aaggcttaag tgcttctaat atgatatctt gtgctttatc atatggtata
     2401 tccatgtcaa atttatctgt caaaggaaca tacaaatcat acatgtgtat ttcattcaat
     2461 cctaaaaatt tctttttaag ctcaacatat ttatttagat taggaatact tttatctata
     2521 gctgatataa gattattgta gacatctaaa cttacatcat ctgaaaataa agacgcttct
     2581 attgctgaat tatactttct agtttttgcg taaaatatct ctgattttat tccaccataa
     2641 agagttgagg caaatgtatt tttatatttc tcatatgttg aaaattcacc ttcaaaagcg
     2701 tcttttctaa ctctactatt cttacttttt ataaataaag aatagtttga atgagaaagc
     2761 ttgaccttat taccatcttc atcttctatc tcaggaaatt gcatatctgc atatgctaac
     2821 atatcgtata cattttctgg aactgtcgat aattctgatg tagctgcaag aatttcttct
     2881 tctcgttcac tcaaagtatg tggtttatct ctcaatatat cctttatata ttttgtataa
     2941 aaagataact tatcatcttt taaatattca cttaacttat tctcttctat agccagtatt
     3001 tctggaacta tataagaagt agccacaccc aaatcagtag ataacatttc tgtcttagta
     3061 gctcttcctt gattatcatt aattctagta tcttcatgtt gtttcatatg agtatataca
     3121 tataaatttt gaacaatcct agatgcattt tcagagatat ttaatacttt atataagttc
     3181 tccttactat cagaaagctt ccctttatat tcttttactt cttggataag attttttact
     3241 ttgcttatgt ctttttcaat ttcctctttg ttagagtaca tcttatctat cttccattta
     3301 tacttttctt ctattgtact tctatcagtt cccatatcat acacctctat tttaatttgt
     3361 tttatatttt attcacttta caaaatatat tgtatatatt ttagtccaaa taatctacat
     3421 ttattactcc ttctatcata tttatttctt tcataacatc ttgcaagtgt attctcttag
     3481 cataagatac aaaaaaattg accttccagt attcttcatt acgacattct atttccatat
     3541 ttcttatgat aagttgactt ttttcaaatt tatcatataa aaaaggtata gagtccaaat
     3601 tgcttaattt taatatcaat acattttttt cttgactaaa taaaaatgta ttgattatcc
     3661 taagcacaaa taatgctatc attacaaata atccacctag aattgccaat tcataataac
     3721 catatccaac tgctacacca atacatgcag ttgcccacat accagcagct gtagtgagac
     3781 cttttactcc acctttattt tttaaaattg ctcctgcacc taaaaaacca attccagata
     3841 atacttgagc aggcattctt gctgggtcag catttgtata caaattataa tcaaagaata
     3901 attgaatcga agttattgaa acaatacaag aacctattgc aattagtata tgtgttctaa
     3961 atcctgcaaa ctgtcttatt ttttctctct ctaaaccgat tattcctcca attattaatg
     4021 ctaatagtaa ccttaatgct atctccttac ttcctatatc aatcataata taaaaccctc
     4081 ctgacttaat tacaacctat gtcctacaga taatttacac atcagaaata ctattttata
     4141 tcttaatatt attattaata tatgtaaaat atagccatct gttaattttt gatacaaaaa
     4201 aacccacaac tgtgggctat ttgcttaggt acatgtattt atttattaat atatctattt
     4261 tcttacttga ttctactaac tcttcttgaa atatttctgg atattctatg tatgtattta
     4321 tctcttccct aactgcttca atctcatttt ttaacgcttc taattcattg ttcgccataa
     4381 taatcaccac tattacaaat ttgtatattt ttatatacat tttatcaaac aatattatat
     4441 tttgtcaata ttatgtcaat aaaacttact ataaaaatgt tttaatttag gtattgtata
     4501 cctggatact aaacctacaa acataccagt tactaatgat gccattaaca taaatggtaa
     4561 gtaccctata gcaagtatat tttctatgat taaacttgca actattaatt gacctatatt
     4621 atgccctatt gcacctataa tgcttatccc tattattgat aaacctttta ttttgctcgc
     4681 taaatacata aataaaagac tcagataagc tcctcctata ctaaatagta gatatgataa
     4741 agggcctgca aatattgatg acaaaattac cctgatagta acaattataa cagtatcttt
     4801 gacacctaac aacattaatg aaattaaagt tataatattg ctcaagccca atttcgcccc
     4861 tggaaatata actataagag gatttggtat atatgtctct agtacatata aagctaaact
     4921 ataccctatc ataagaccta aaaatgtcat ttttctagtt ttcattaagt taactcctcc
     4981 aaattttatc tatcatctta cattataata caaaatatat aaaaacacct aatttagact
     5041 aaaatatttt ttaatctatt tagaataaat attagttatt tcaatgtgca attacctcta
     5101 taattcttga ctttttgtat catacttgtt acaattttaa tgataatgaa tttcattagg
     5161 aggtgttaat aatgactgtt tacgatttaa aaataggaga aaaaggattt attaatagta
     5221 tagatggaga tattaaatta tctaaaagat tacttgcact tggatgtata aatagtactg
     5281 aaatagaagt taaaaaggtt gccccacttg gagacccaat tataattaga tttagaggct
     5341 ttgaccttgc gattagaaaa tctgatgcta aaaatatttc tttacaataa tagaggagga
     5401 gctacccgtg attaatgtag cattattagg aaacccaaat gtcggtaaaa ctactgtttt
     5461 taatctactt acaggttcaa atcaatatgt aggtaactgg cctggtgtta ctatagaaaa
     5521 aaaagaagga tttcttggaa aagagataaa ggttgtcgat ttacctggta tatatgcaat
     5581 ggatactttc tcaaatgaag aaaaggtttc taaatcatat ttagaaaatg aagatgttga
     5641 tgtaatagta aatgtagttg atgcttctaa tttatcaaga aacctttatt taacaactca
     5701 acttatgcaa tttaacaagc ctatagttat attgcttaat atgttagaca ttgcagaatc
     5761 taaaggagtt aatatagatg ctaaaaaatt aggcgaagaa cttggagtaa ttgttgttcc
     5821 aattatagca aaaaagaaaa atggcgttga taggatagaa gaagagatta agggagctac
     5881 acaaaaagct tttatatatc aaaaaaactt tggaagtgaa acagaaacat ataaaaaatt
     5941 agaatccatt ttatcaagat gtacaaaaac tacatataca cagaaaaaat ctatcagtga
     6001 taagatagac aatatagcct tgaatcctat attagcatat ccgatattta ttggtgtatt
     6061 attcctctta tttaaattta cctttgattg ggttggagga ccgctacaag agggatttgc
     6121 tagtcttata gaggcttata tttctgcacc tgttagtgat atgttatcta actctagccc
     6181 atggtttaaa tcacttatag ttgatggaat aattggaggt gttggtggta ctttaccatt
     6241 cttcccttta atatttacat tattctttgg tatatcgttg tttgaagata gtggatacat
     6301 gtcaagaaca gcatttttaa tggataaagt catgagaaaa gttggtctat ctggtaaagc
     6361 ttttatacct atagttatgg gtatgggatg ttcttctcca gctataatgg caactagaac
     6421 attggaaagt gaaaaagata gaagagttac agctcttatt gcaccactta tgacttgtgg
     6481 agcaaagcta cctatatatg cactatttgt agctatattc tttccacata atgctgcttt
     6541 agtaactacg tcactgtact tagtaggtat agttatggca attgtagttg cattattctt
     6601 aaataaaaca gccttcaaaa cagaggctga accatttata ttagaattac cagaatatag
     6661 aatccctact atagatgcac ttattaaaaa tacttggaat aaatcaaaag gtttcttgat
     6721 aagagttgtt actgtcatgt ttgcaatgtc agtcgtaatt tggggacttt cttcattcaa
     6781 tgtttttgga tatacagagg atataaatgt aagcttcttg gctactctag gtcatattat
     6841 atctcctata ttcaaacctc ttggttttga tgattggaga acttcagttg ctattttaag
     6901 tggtcttgga gcaaaagaaa tcgtcgtaaa ctctttaaat atactatatg gtaatctaac
     6961 tgttgtctta ccaacaatat ttaatggagt tactgcatat actttcttga tatttactgc
     7021 tttatatact ccatgtatag ctgcacttgc aactatgaaa aaagaatatg gcaataaaat
     7081 gatgtttgca tcatttgcat atcaatttat actagcatgg ataatggctt ttatagttaa
     7141 aagtataggt ggagtactat ttatgggcaa ttcaatagtg gaagctttga taggtggaat
     7201 aattatagcc cttgcacttt ttattttgtt taataagttc aagtctacaa aaaacggaag
     7261 tgtgtgtagt ggatgttcta tttgctctgg atgtccaagt gctaattctt gctcaagtaa
     7321 ggaagacagt aataaaaaaa atagtacaca ctaatatata aatattttag tttaaaataa
     7381 cgtagaagtg agtgtcttaa aattaacttt ttagttcatt aggcactctt ttttatatgg
     7441 aataaaatat atttttatca tttcaacagt caaaaagagg cttatctata ttttgagaca
     7501 gcctcttatt ttttacttat tcaatttctt gctacctaac ttcatatgga acttagtatc
     7561 ttttgggatg cttgggtcta ttccccttaa tgtaggtatt tccccacata ttagttgtat
     7621 aggtactata aaattaaatg tttgatagta aggattatct gtaatatcta tttttaaatt
     7681 tttatcattt tctgtaacat ctctacctat agcaaataca ttttccgtcc aaccagaaag
     7741 tacatcaatc atcttagtta ctctaggttc ttttcctgta tctagtataa atatagtaga
     7801 atcactattt atagcattat atataccatg tataaattct tcaaattcat acccagttac
     7861 aggtattctc attgtttcca ataatttcaa tgctgcttct aatgtatctc catatatatc
     7921 agagtggcct attattctta tttctttaga attaactaac ttttttttgt ttctttcaat
     7981 ccattgttta gacaacttat acactgcttc aaatctatta attgcatcta atatcttatt
     8041 tatttcatca ttgtactttt cactagatat tagtcctttt tctctagcta tttgaagacc
     8101 taacaacatt aagtttaact ttgtacaata atatcctttt gttttagctc cagatttttc
     8161 ttctccacaa tttactgtta atatataatc agaaatctca tctattagag catttttaca
     8221 acccgccata gatgctattt tacacccctt gtcttcagcc aacttcatag cattgtatgt
     8281 agaataacta ctcccacctt gagatacacc tactactagg gtattttcat tatcaaactt
     8341 gaatgtatct tcagttatca taaatggata cattttcaca acatctatat ctaataagtt
     8401 ttgtaaatat ggttgcactt gcactcctga gtgatagcta gtacctgaac cagttattat
     8461 aatctttttt agattttctt tcttaacttc attgaataag ctatctgcat tagatataat
     8521 ttctctcatt ctaactggtg tttctaacat ataatcttgt attgtcattt tatacgcctt
     8581 ccttatctat atattcaata aattacaatt aacttctata acaaacctat agcatgtaaa
     8641 actattcctg ctactatagt tcctatagtt aattttgtta cacttacctt tctctttaac
     8701 atgtaatata ttacaaatgc aaaggttaaa ggtaacatat taggtattat tttatctagt
     8761 atatcttgta aaattacttc tgcaccattt aagttaaatt tcagaggagt agttatactc
     8821 aacatagttg caaccattcc tcctacaaca aataaaccga cagttgtagc catagacatt
     8881 attttatcca taagtccttc tctttgtatt ctatcaagag aatttactcc tacttgataa
     8941 ccatacttaa gtccaaatat tctaagagca agtgctggta tattatataa tagtaagaat
     9001 aatagtggtc caaagatatt accttctttt gaaagtgata ctcctactcc tgctgctata
     9061 atcctaaatg ttccccagaa tagtgaatca ccaattcctg caagaggtcc cataagagct
     9121 gttttaacag cactaattga actttcatca aattctggat tattagcatt ctcttcttcc
     9181 atagctgctg ttatacctaa tatagcagga actaccactg tcgttgtatt aaaaatctcc
     9241 aagtgtcttt ctattgcttt tgcttgattt tctttcttat tgtataactt ctttattgca
     9301 ggtaacattg aataacagta acctaagttt tgcattcttt cgtaattaaa tgcaccctga
     9361 agagcaaatg aacgccaaaa catacttctt aattcttttt ttgttaattt attcaaagtc
     9421 aatctctcct tctatcaaag tattaattgc taattgagga ttttgattat tttctttgtt
     9481 attgatataa aaatttagta caaatgctac acatactcca aataaagcta tagcagttat
     9541 atccaatcca ccataagatg ctaataaaaa ccctataaag aaaaacacag acattttttt
     9601 tgaaaataac atatccataa gcaatgcaaa acctactgct ggtaacaatg ttcctgcaac
     9661 acccaaaccg tctaatataa ttttaggtat tgcatttatc aatgctgtaa ctttatcagc
     9721 accaatcata attgctaaaa aagttggaac tgatgtactt aaaaagaata atattactgg
     9781 caaccacatc attatggtaa ctctcttaaa atcagcctgt ttagcataga aaactgccct
     9841 ttgagaaaaa tacgaattta ttattctaac cagtactcca agagattgtg ctaagactgc
     9901 tactggcact gctactgcta aagcaacttc tgcaccatta cctgataata tagcaaaagc
     9961 tgtacctaat acacctcctg ttactacgtc tggaggagta gctgcaccta ttcctgtaat
    10021 tcccatccaa attaattcaa gctgtgcacc aatcataatt cctgatttta tatctcctaa
    10081 aactaatcct actaacatac ctgtaactat cggcctatca aacatagatt gaccaaatat
    10141 acgcccatct aatataccaa ctcctgctat aatagctaac atagtagctt gtactaacat
    10201 agttaacctc cattttaact tagtttaatt tacattaaat tatatttttg attttgtttt
    10261 gattttctaa taactcattt acataaacaa ttccattttt tgcttcctct aagcctgatt
    10321 taattattag ttttgtatcc tcttcaacta ataaaagtat agataaaagc attggtaaat
    10381 ttactcctgt caataattca aatttgaacc cttgtaaaag taagtttgta catatgtttg
    10441 ccacacttcc accaaacaca tctgttaata caacaaacgc ttcactatct ttattattta
    10501 ataatatact tctaatttct ttttcagcat catctgctga tttatccttt gtcatacaaa
    10561 aatactcaat attagttaat gaaccaatta tcaatttact tgtatcaaca agttctctag
    10621 acaattctcc atgtgtcgca attagaaatt ttttcattct ctttctccta aattatttta
    10681 ttaaatttga tagttgtttc tttttatctc taggtaacat tctaaattct atatcaattc
    10741 ctaaatcact tatttcttta aagttattta tatcatcatt atcaacagca actaaatctg
    10801 taagtttctt ctttccttct tcatatctta accctcctac atttatttct tttaatactc
    10861 ctcctgagtt tttagctata actaaagcat ctttggtatt ttttactaac actaatacat
    10921 tgcttttctt tgaagaagca aatttttcta ctatttctcc agactcctct acccccctta
    10981 tatataaagt tacattgcta ggtttagcta aattcagtga catagctttc atttggtcat
    11041 tttttgcttc atcatttgca actagtatta catttatccc tagtgatgtt gcccatccat
    11101 atgccacctg accatgtatc aatctgtcat caattctcat taaagatacc attttcctca
    11161 aatcctccat catacattaa aatttttatt gtttgtcctc taatttatct ataaaagcaa
    11221 aaagtatgcc aaatactggc atactttttc gaatttcatt cacttatttt gtaatcttgt
    11281 attttactgt ggtagaccat agatgatatt ataaatgtat cctatctctg aaattgatat
    11341 ttgcacacta taactctttt ctagcacact aaatgcattt tttattaatt ttacttcact
    11401 atgatggttt ttgattaaca aatccatatc agtatactct tcaatttcag cttgtctaac
    11461 taatctttca atcatgcaac ttaagtgaac atataaagca atttttttat catttggaag
    11521 ttttttcttt tgaatatttt ctatagcgtt gattccctct tctattctct ctattatctt
    11581 ctttgaatct aaaatagtta gactgtctat tactctaatt aatgaaaaat tctttacaat
    11641 agaatcattt atgctcttta ttttttcatt ttctactact ccactaaata ttctaaaaac
    11701 atcatcttct ccttttcctg atattatatc ctctaataat ataaaatcca ccccattcac
    11761 atttggattg gcagttccta ctatagccag tacatcatac atttcaaaaa ctggttcttt
    11821 atctcccata gaaacaagac tatcataatc ataaggtatt attttgacat caactattcc
    11881 aattaaactt tctgaaagta ggtgttggat ttttatagca gtacctatcc ctgtaaagca
    11941 agatgatatt atagcatatt ctttattttt ttgaggataa tacaattgta tattaggttt
    12001 agtctcttca tccataattt ctgcaatctc ttcaatgttc ttaccttgca ttaataaatt
    12061 tcctacttct agtgcagaaa tggtggatat ttggtctata aataacattg tgctatttat
    12121 cttactcttt aaatcttcgt atataagatt aagagagccc atatcaaata acacaattaa
    12181 accattcttt atcctatctc cctctaggta tctagacatt tgttttgata catcagcaat
    12241 ggtagcatct ataggcatat caatagcagt gaatatattt ttacccaaca ttcgattaca
    12301 tacattcgcc atactactgg cagtagcata accatgtgct ataataatcg cattaacctg
    12361 attaaatttc attcttataa ttgtcgactt aataaatata atcattatga ttgtatctac
    12421 attattaaga tacatatcca atctattcat tattaatgtt gatattttac ttattataga
    12481 gtattctttg taactctctt tttttaaata tattagcaat ttattttcta aagctttata
    12541 ttcatcagac aattgatacg attcatcttt caaataaaga taagacgaaa atgccaatac
    12601 attattccca tcatatttta tattgttatt aacttctaaa tttctaaata tatcctgcat
    12661 tattgatatt ataagctcca taagtacgtt attattttca tcattatttg aaaaaactaa
    12721 attatccatt aatctagtca ctagattaga actcttttta ctaaatcttt cttttgttat
    12781 ttcatttttt tgtagtctat caaagtaatc taaacatttt ataaaacttt gatatattat
    12841 gcttctttta tttgttccat ttgacatttc aatactaaat tgtttaggtg aaaagacaat
    12901 gcttttgctt ttaccacttt gaaattgaaa tatgtctaca ttttctatat aaagatttct
    12961 aggaaggtct tgaagagtaa cgaaaatatt tccatttctt ctttgatttt ttgcataagc
    13021 tcgtccacat gcatatttta ttacattttt acattcgcct atattcccct tgaattttat
    13081 ctttaaaatc gtatttaatg taatttgagt tatctctata ggcaaaccaa gtaccttact
    13141 ttcctcataa ataaaatgtt ctacaatctt tcttttttca atactccctc tttcttctat
    13201 attaggaata taaatattta tgggtattct tcttaaaaaa gtttgtaaaa actcagatag
    13261 gctctctgtt gtagcaaata ttaatcttac cctagctttt ctaattactc cattctctcc
    13321 aattctggaa aacgtacctg tatccataaa tgtaaataac ttttcctgac cttcactatt
    13381 taatctatgt acttcatcta aaaataaaac accgccatca gactgttcta ttagacctgc
    13441 tctagattca tttgccccag tatatgctcc ttttacatgc ccaaataata gactagacaa
    13501 taactccaaa ttattataat actgcgcaca gtttaacaca ttgtatggag aatttttttc
    13561 taatacatta ctacttacac tatatttata tattatcttg gctaaaaaag tttttcctac
    13621 tccactttca cctgtcagca ttatcgtaag cccaccattt ggataaagca cagcagtttt
    13681 tatttgttcg attacctctt ttaagctatt atcgtatcct atgatactat taaacacaat
    13741 ctcttgcttt aataatttgc actcattttt aagttcatct aaactttcat aaatactttt
    13801 ttttgtaggt cctactacat catttatctt acttttaggt aaaaaaataa caggtctact
    13861 atttatcttt ataagattat cagattttac taagtcattt aataatctac ttgattgagt
    13921 tctacttaaa ttaaactttt ttactatgtc ttctgtcact atgccacttt cttttttaag
    13981 ttcatcaaag ttgttaaata atgcacattc atttacaaaa cttaaaattt catccctcat
    14041 gttcaacctc caatacaaat atcaataata atataatatt agtatatcat ttatatattt
    14101 atgaaaaaga taattatatt cctatcttaa ttctcttttg taagattgta aaatcaaact
    14161 ttttataagt ataaaatcta gattttttaa cataatctca agattcttat cttcctatat
    14221 atttgataca gagtttatat aataatatat ttattgtatt tagtataata aaaagtaaaa
    14281 cattataggt attttaaaac gtattagtga agaaaatatt tgtaaccttt attatattat
    14341 gaatattatt tcagatttaa aaaggctata aataaattta tttttaaata aatctactta
    14401 tagcctcaca ataatactta taaattttat ttttccacaa taatgtatct caaatatagt
    14461 taaaaatata tctatttaat tattaatatt taagtaataa agtctcactc aatcattaaa
    14521 ttccttttta tagcaaatta ctcttatttc acttatgctt aatacattta tagatataaa
    14581 tactaaaaaa gcaattcaat aaaccatatg ataaacctaa tttatgtctg ttatttgctc
    14641 atatctttag acttatctat acaagcaaca atagatttta taactgccgc tctaaatcct
    14701 tcttcttcta ataccttaac agcttctata gtagttcctg ctggagaaca aaccatatct
    14761 tttaactcac ctggatgttt tccagtttca agaaccatct tagctgaccc cattactgct
    14821 tgagatgcaa atttataagc ttgttgtctt ggcattccag ctaatactgc tgcatctgcc
    14881 atagcctcta taaacataaa tacatatgca ggagaagagc cactaatacc tgtaactgta
    14941 tccattaaat attctgatac tatctctgcc tcaccaaaag attcaaacat agccttaatc
    15001 atatttaact cttcatctgt tacattacta tttttacaaa gagctgacat tccttcccca
    15061 acaagggctg gagtattagg cattactctt actatttttt tatctgagcc tataaaactt
    15121 tccagacttt ctatagtttt acctgctgct atagatacta ttattttatt tgcatctatt
    15181 aattctttaa gtggagttaa agccttttcg catatattag gtttaagtgc tactattacc
    15241 acatcagaat ttgcaacagc tacctttgag tcagttgtag tatttactcc aaattcttct
    15301 tttactttat ctaaggcaac ttggcttaag tctgatacag ttatgtcttt tgcttttact
    15361 aatcctgact gtactatacc acctatcatt gcacttccca tattacctga accaataaat
    15421 cctaaagttt tcatatcaaa tctccttttt tctattagtt ttattttaaa gtagctaaat
    15481 taactttatt ttaactgctt aaaattatct tatttttata aaatataacc ttattataat
    15541 tataacaaat tacttttttg tatgtattac ttactaatat atgtatgatt tattaaatat
    15601 aaatttaacg attttttcat taaacttaac gagctaactt cctgtttaga aagttagctc
    15661 ataattaatc taaaattttt aaaatataat caaaccattt atactttatt atatcagtta
    15721 cgattttata tagtaagaag ttttatttgc aagcaatcct agaatgtttg ctcagttctt
    15781 cctattatct catcttgtag tgttctactt aaattattga aatggtcact atatcctgca
    15841 actctaacta ttaaatcttt ataatcctga ggattttttt gagcctctaa cagtacattt
    15901 ttatcaaata cattaaattg tatatgatga ccatccatat taaagtatgc tctcactaga
    15961 ttagccatat tgtctaaccc tttttctcct tgaactactg atggggcaaa tctttgattt
    16021 aatagtgttc caccagtttt tatgtggtcc atcttagcac aagattttat aactgctgta
    16081 ggcccgttag tatctccacc tttttcaggt gaaattccct ctgaaactgg ttttgcacaa
    16141 agtctaccat ttggacttgc tcccataatt tctccaaagt atatatgaca agttgttggt
    16201 aacatatcta ctctgtattc tccaccacaa acagttggtc taccagttac ctcattgtaa
    16261 tatgcattga atatctcttg cattatctca tcagcataat catcatcatt tccatattta
    16321 ggagtcttat ttgaaactaa attaagtata gcttcgtatc cttcaaaatt agcatcaagt
    16381 gctttaagta atgtgtccat atcaaatttt ttcttatcaa atacattata ttttattgct
    16441 gtcaaactat ctgttatagt tcctatacca acaccttgta tatatctagt gttgtatctt
    16501 gctccacctc tttggtaatc cttgcctttt tctatacaat catcaactat tgtagacata
    16561 agtggacaag gcatgtgttt tgcacatata ttttctatca cagcatttcc ttctattttt
    16621 atgtcaatca tataatgtaa ttgcttttta aatgcttcaa atagttcctc ataagatttg
    16681 aaatttctag ggtctccagt ttcaatacct atttgttttt tactaattgg gtcatatcca
    16741 ttatttaatg ttaattcaag tatttttgga atgttcatat atccagttaa aacatatgct
    16801 tctttaccaa aacatccagt ttcaacacaa ccactagttc caccaagtct tgcatcttct
    16861 atagtttttc cagcttccat aagctcttga actattgctt cagtattata aaatgctggt
    16921 tgaccccatc cttttcttga tatttcacaa gctctcttta ggaatttttg aggagtcttt
    16981 ttacttattt gaacatttga actaggttgt atcaatttca tttcatccat aacatcaagt
    17041 attatataac taacttcatt aacaccatct tgaccatctg gatttattcc accagtgttt
    17101 atatttgcaa aatctgtata tgtgctactt tcttttaatg ttataccaac ttttggtggt
    17161 gctggttgat tgttaaattt aacccataaa cattcaagta attcttgtgc tctatctcta
    17221 tctagtattc cattttctac atctctttca tagaaaggat ttagatgctg gtcaagtctt
    17281 cctggagtaa aagcatccca tatattaagt tcagtagtaa cccctatatg aacaaaccaa
    17341 tacatttgta tagcttgatg ataagtttct ggtttgtgag ctggtacaac atcacaagtt
    17401 tcagcaatta gtaataaatc ttttttctta gcttcatctg tttcttcttc tgccatttgt
    17461 cttgctttct cagcatatct tttacctaat ataaccattg catcacaagc tattgccata
    17521 gcttctaaat cagctttttt attatatgca tctaaatcat taagaaaatc tagctctttt
    17581 aatcttgctt ctatatcttt ctttaaatct aaaaatcctt ttttgtatat tgtatctcca
    17641 caaactgtat gtcctggagc tctttgctcc ataaactctg taaacatacc tgcttcatat
    17701 gctgctaacc actcaggtgt cattgcattt attatcttat ctcttgtttg tctctttttc
    17761 caaaaaggaa ttatttcttc tttatgtatt tttctagctt cttcagatac gctaaaattt
    17821 attatatctc tattgtgcat tacttctaaa tcttccattg tatgacaaca aatttctgga
    17881 tatgttggtg ctccattagg agaatcacct ttttcaccta ctattaattc tccatcattt
    17941 atgctaagag ttctattctc aatataatgc ttaaatgata aagcacgaag tacaggaatc
    18001 tctacacttc cctcatattt tttataagct tctgtcataa gtactgctct ttcaatagat
    18061 atgtgtggtt ctgcattaat actttcttct cttaattttt tagttctctc aaaagttcct
    18121 cttgccattt tattaccctc caatctcagt attgaaatta cttgcttcaa aaagtctttt
    18181 tatttcctct aatttttctt ctgatggtct ttgtagttct tctccttcat actttttata
    18241 aatcttatca tacttatgtt taccaatatt atgatatgga agaagactca ctgcttgtat
    18301 attcaaaggt tttaaaaact ctatcatttt tttaacctct aaattttcat catctacatt
    18361 tactcctaca attaatggta ttcttatatt tatgttagcc cctaattcac ttaaaacctt
    18421 aacatttttc agtattaagt catttgattt tcctgtaaat tttatgtgct tctcttcatc
    18481 aataagctta atatcatata aaaacaaatc tgcatattta gcaactcttt cataattttc
    18541 tgactttgca tagccacatg tatcaattgc tacatggatt ccctttgatt tgcacatatt
    18601 tattactcca caaatgaagt ccatatcttg agccattact tctccacctg acagagttac
    18661 tccaccacct gattcttcat agaaaattct atccttgtct atctctttaa ccaagtctct
    18721 tacagaatac tcttgaccaa caatctccct tgcattgttt acacaatagt ctaagcaagt
    18781 ttcacaaaat tcacacttat cttggtctag gcatattttt gtttcacctt tgtaaattgc
    18841 cttatgaggg catatattta tacatgcttc acatcttgaa catctttctt cattatacaa
    18901 aacttgtttt gtatatgttt gactctctgg attatgacac caaacacatt caagtggaca
    18961 ccctttaaaa aatactgtgc ttcttatccc tggtccatca tgtatgctgc atttttgtaa
    19021 gtttataact aatggattca taatttttca cccctaaata atatccattc cattttttca
    19081 tttttatgta atttatcttc atagaatata cctatgaaaa gcatttctac aattacataa
    19141 tactatactt gttattattt tgtctatatc tttttagtaa tattaaaaaa ttttatttta
    19201 ttatgatatt tatattgttt ttgtaaatta aatttaaata tttattaaat ttatttttat
    19261 aagaaaatat agcaataagc cataaacaat acatcatttt aattaattta gatgtatata
    19321 ctataaaaat taaataaaaa tagagactac actttaccat gtagtctcta tttttctggt
    19381 catatttaga aatttacatt tattgttact gcttttccac ctctatttac tgttattttt
    19441 gtactagctc cttttgataa ggtatacaac tttttattta aatcattcat acctgttatg
    19501 tctgtgtctc ctactttagt tataatatct cctaccttta caccagcttt ttctgctgat
    19561 gaaccagata ttacttcagc aacataaaca cctttatcag tacttaattt tgtacctgtt
    19621 gctgcttcat aatttgatac atctgtacct tttataccta aagttacttt ttcatatttt
    19681 ccagttttta ttacttcttc aactatactc ttagctgtat tgattggaat tgcaaatcct
    19741 aatccttctg ctttagaagc ttttgctgta tttataccaa ttacttgacc tttttgattt
    19801 aataatggcc ctccactatt tcctgcattt atacttgcat cagtttgtaa caaaccagtc
    19861 atagtagttt tttctgtttg tatagttcta tctaaaccac ttattatacc ttgagtaact
    19921 gttttttgga aatccaaccc aagaggatta cctattgcta ttgaaatatc tccaactttt
    19981 actttatcac tatcagcaaa ttctgctggt gttagcccag ttttatcaac cttaactata
    20041 gctaaatcaa gttgttggtc aaaccatact actttacctg aagttgttga accatcattg
    20101 aataatacat ttacacttgt agcttgacca tctgatataa catgagaatt agtaagtata
    20161 tatccatttg aatcaactat gattccagtt cctacaccct gtgtttcagt tggtattgca
    20221 aacatattat tagtatcaac actagttgtt gtaataccta caacagatgg tgtagctttt
    20281 tctgcaactg cttgataaat attttgactc ttaccatcat catttacaat tattggtgta
    20341 gaacttcctg tagatttgcc tactaaatta tccttaacta aaattattgt tagaaaacta
    20401 ctaagtattg aacttattat agcaactaat attactaaac ttatcccttt ctttcttctt
    20461 gacatctcat accactccct atctaatata atataaatta tcaaaatata tttttcccaa
    20521 tttttcataa aaaaaatgaa atcatctatt ttgagtaaac tccttttcga tgatttcatt
    20581 atattatttt aatcttaatt aaaccttaac tatttaaaat tatttaagtc ttttttctag
    20641 ctcttccttt tctttttcat aacctggttt tcctagtaaa gcaaacatgt ttttcttgta
    20701 agcttctact cccggttggt caaatggatt aactcccaat aaatatccac ttattccaca
    20761 agccttttca aagaaataca ctaagtatcc aaaattatac tcatctaatt gaggtatatt
    20821 tattactaaa ttaggtactt ttccatctgt atgagccaat agagttcctt caaaagcttt
    20881 atgatttaca aaatctacag tcttccctgc tagataattt aaaccatcta agtctacttc
    20941 ctctgaattt atagttatat ttctcttaca attttcaaca ttaagtactg tctcaaataa
    21001 aagtcttttt ccatcttgta tgtattgacc catagaatgt aaatctgttg agaaatctac
    21061 agaagcaggg aataaacctt tattttcttt tccttcactt tctccataaa gctgtttcca
    21121 ccactcactt acatagtgta gttgaggttc atagttaacc aataactcta catcttttcc
    21181 ttttctatgt aaaattgttc tagcaacagc atatctataa ctatcattat tttttaggtc
    21241 aggattttga aatgcttctc tagcatcatt tgcacctttc atcatagcat caatatcaag
    21301 accagcagca gcaataggaa gtaatccaac tgctgtcaat acagaaaaac gaccacctac
    21361 atcatctggt attacaaaag tctcatatcc ttcttctgta gctaactgtc taagtgcacc
    21421 tttacttgaa tctgttgttg cataaattct ctttcttgct tcttccttac catacttttt
    21481 ctctaagtaa tctttaaata ttctaaaagc tagggctggt tctgtagtag ttcctgattt
    21541 agatattaca tttacagaaa tatccttccc ttcaattata tctaataaat ccataatata
    21601 tgttgagctt atattatgcc ctacaaaata tacttctgga gcatttcttt gctctttttt
    21661 aagtctattc ctaaaagaat gacttaccat atctatagca gctctcgaac ctaaataaga
    21721 gcctcctata cctattacca aaagaaaatc tgagtctgat tttatcttct cagcagattt
    21781 ttttattctg tcaaactctt ctttatcata attcttagga aggtctatcc atcctaaaaa
    21841 agtatttcct aaaccagttt tattatgtaa catgtcgtgt gcaacatcta catatggttg
    21901 cataacatct atttcgtttt gacaaaaaaa ctctgtagct ttactataat caaaatttat
    21961 ttttctcatt taaatccctc ttttcagcat attcgtttta aagagattat aacacaaaat
    22021 atgggcgttt tttttaaaag tttatcacaa aatcatacat atctcatcat ttattatcag
    22081 gaaatcgatt cttctaaatg ctataaccat gtacaatttt ttaaatttta gtcaaataaa
    22141 tttgcaaaaa aagaaactct aaacgtatca tttaaagttt ctttttattt atattctatt
    22201 tcaattttta tattgttcta aactaactaa ctttaatcat tacctaatct cttcgctata
    22261 acttgttgtg ctatattgac agcatggtca gatattctct ctagattact tattacatct
    22321 aaataaataa ctccattttc tatactacat agattattgt tcaatctata catatgatta
    22381 gctctacaag atttctccat tatatcaacc tgctcttcaa tttttataac cttgcaggct
    22441 aaatctacat tatcagttct cattgattct aatgcataag tatatgctga aacaactcta
    22501 ttatacataa catcaagttc agactgtcct tcttctgaga aagaaatatt tgaatctatc
    22561 gcaacttgtg ataattcagc tatattttta gcgtggtcac caattctttc tatatcgttg
    22621 actgtattaa acaaggtatc cacaacttct cttgaatcat catctaaagg tgcttttgaa
    22681 agtttaagta agtaattcaa aatagctttc tgtaaatcat tgattaatct ctctcttcta
    22741 tatgtcttgt ctattttttc ggttgaatgt tctactaaag cttccataga tgcatttaaa
    22801 ctttccttag ccttttcacc catacgaagt gcttctttta tcgtatttgc aagagctata
    22861 gaaggagttt ctatcattct atcgtctata tatttgatag tcttagtttc atcttcatct
    22921 atgtcacctg ctctatcagg aacaagtttt atagctagtt ttatgataaa atttgtaaat
    22981 ggtaaaagta tcaatacaga tattatatta aataatgtat gtgtattggc tatttgtctt
    23041 gcaacattgc ttgggtccca gcttgtaacc atgcttacta ttggtttatt caagaatatt
    23101 aagaatatca ctgttcctaa aacattgaag attagatgca tcatagctgc tcttcgtgca
    23161 tttttgttgg caccaatact cgataacagt gaagttacac atgtaccaat attttgacca
    23221 taaagaattg gtagtgctgc tgataatggc actagacctt ctgctgccaa tgcaaccaac
    23281 atacccatag atgcacttga actttgtatt attgctgtaa tcccaaatcc caataataat
    23341 ccaagtattg gatatctacc aaatgtaaca agtaagtcag taaatccttg atagtctctc
    23401 agtggctcta ctgccatctt cataaaatcc atacctgtaa ataatatacc aaaacctata
    23461 agtatttcgg ctatattttt tattctagga ttatttccaa ataaataaag tataatacct
    23521 acacctaatg ctattggagc cataccagtt aagtcaaaag ataccaattg ggctgtaaca
    23581 gtagtcccta tattagcacc cattataact cctattgcct gttttaaggt cattattcct
    23641 gcatttacga aaccaacaac cattacagta gtagcacttg aactttgtat tactgctgta
    23701 actcctgtac ctaccaaaac tcccataaat aggttactag taagtagttt tattatcttt
    23761 tttagctttg tcccagcaga tttttgaaga ccttctccca taaggttcat accatataag
    23821 aacagaccta atccacccat tagacttatt ataatgttca aattatttcc tccttgtaaa
    23881 gagattactg tatctaaaat tttataacct attgtatttt atcaaattat aggagtgata
    23941 atgttaaatt catgttaaat attaaaaaat cattaagttt atgttaaatt tttgtattat
    24001 tggtgtattt ttgtattatt ttttctgcac attttatgcc atcaactgct gctgtgacta
    24061 taccacctgc atatccagcg ccttctccac atggatatag attcctaaca ctcactgatt
    24121 ctaaagtatt ttcatctctt actattctta ttggtgatga tgacctagtt tctacgcctg
    24181 tcaaaacagc atcatacatt gcaaacccat tcaatttttt atcaagagat ataaatgctt
    24241 ctctcatagt ctctgttaca aaactaggca aacattcact taaatttgta agtgtatatc
    24301 caggtctata tgatggttca acatttccta aatttaaaga agtcttatta tttataaaat
    24361 ctccaacaag ctgaactggt gcattgtaat tttttccacc taattcaaaa gccaattttt
    24421 catacttctg ctggaaatgt actcctgcta gtggattatc actcttaaaa tcttctggtg
    24481 ttacatttac taaaaatgca ctattagcat ttattttatc tcttgcatgt tcactcatac
    24541 cgtttgtaac tatttcaaaa tcatttgatg tagatgctat gacgctacct cctggacaca
    24601 tacaaaaagt atatgctgtt cttccatttg aaacatgctc tataagtcta taatccgcag
    24661 ctcctaatct aggatgattg taaaagtctt tgtactgtgc tttatttatt aaatcttgtg
    24721 gatgttcaac tctagctcct attgcaaaag gtttttgaac tatttttacg cctcttgaat
    24781 ataacatttc ataagtatct cttgcactat gcccaacagc taatataatt gtatctgtat
    24841 ctattttttc agtatcattt attactacag attgtataga atcatttgat acaactatat
    24901 ctgttacttt tgaattaaac ctaacttgac cacctaaact tattatttca tttcttatat
    24961 ttttaactac attttttaat atatctgttc caacatgtgg cttatgtgag tataatattt
    25021 catctggtgc tccaaattga actaactctt ctaatacttt tctacatctt atatctttta
    25081 ttctagttgt aagtttacca tccgaaaaag ttcctgctcc accttctcca aattgaacat
    25141 ttgaattatt ttttaatttt ctagtagtcc aaaaatcact tatatctttg gttctgctgt
    25201 ctacatcaag acctctttca agtatgattg gttcataacc catttgggct aaaagcagac
    25261 ctgcaaataa accagctggt ccactaccaa ttaccaaagg attattcttt aattttttat
    25321 cacccatagg tacatttata tatctgattt cctttatttt tgttacatct tttatttttg
    25381 atttaagtat tttattttca tctttaactt ctacatctac agaataaaca aattccattt
    25441 ttcctttttt tctagcatct atagattctt tatatatact atatttgatt aagttttttt
    25501 cttttattct tagcttctta agtacaatat cttttaataa agatatatct ttatcaattc
    25561 caagttttac attagatatt cttaacatat tgttttcctc tcttagattg taatataaaa
    25621 aagctagggt tactcctagc tttctacaaa tataacttct atattctctt ttactagatt
    25681 ttctacccaa atctcatcta gcacaaaatt ctttattttg tttacaacaa gtgttttatc
    25741 taaatctttt ttcaagtttt ttactaagac cattgcttca accgatttat gcaatctttc
    25801 aacatacaga gaattaactt taatcaatac aacccttaat tcacacacat ataacttttc
    25861 attacctaag gtgatttcat catcatattc gtatacacta aattcatatg tattttcata
    25921 aggtgttata ttttctattc ctatataatc catttattta tcctttttta gttataagac
    25981 ctcttataac cttcatgtta ttaaacacct tttctaagaa gttctctgct tcttcattgt
    26041 ctaaaaggtt tatttcaagg ttttcgtttt ctacaatacc tttagattta cagtactctt
    26101 tagcaatatt tattagttca ttttttttat ccatagataa atcaaactct ttatagtcta
    26161 ttattatata ttgtttggta tcaggtgata caccattatt atatcctatt tctatatgat
    26221 aaataactgc atatatttct tctccgttgt acacaggtac agctgggtta gattttataa
    26281 agttcgttga tacagcaatt gcgtttccta aactaagact cattattaat acctctttct
    26341 aaacattata catatattat actatagatt ctttatctat gacttaaagc tttttataat
    26401 taattaagat atttttaaat ctttgtatct ttttcctagc gtactagagc ttatatcgta
    26461 tttctttgat atttgaactt gagttatagg ttgtcctgct tctttcttaa cataatattc
    26521 aacagcagct gcccaaccat tttcagctcc ttttatattc tcatgatttt ttttatattc
    26581 attccaaaac tctatacatg catctatatt ttctttttca acattttctt taagagtatc
    26641 taaaacttta cattcctctt cagctaattc attttctgaa ttaacttttg tatcgttttt
    26701 atctctctct ttctttaagt attcagctat acttgggtct aaaagctgtt gtatatactt
    26761 gtatagtata tgtgtatgat aaattaagaa tgacttcata tctttgtata catcttgata
    26821 ttgattgaat agattcataa tatcctttaa aattatatct ttaacctcat ttgatatact
    26881 tatagttata tcaatcaata tgttagttcc ctcaatatca actattctag ctatcataca
    26941 acttccgatt ttaaaacttc ttagaagttt tacatcttct gtacaaagtt catttccact
    27001 taaacagtcc tttaatacta tttttccagc agaaatttct tttacttcat aaatacttac
    27061 gtatgattca aacagacttt ttagtattga tacttctgac tgacttaagt ttgcaccatt
    27121 ttcttcaaga aagccaactg tcataacctt tttattttcc attatgtggt cttgtattaa
    27181 gtaagtatta aaaaatcttt caaatttttc atttacacta tcattttgca ttatataaaa
    27241 catttcttta gctttttcac attcttcttt aaacttatcc tgtcttgaat aatcatataa
    27301 ctttgaatat aaatctgtat attgtttttg tgaaagttca gtttttctgc atgctctttc
    27361 tgaaacaata tctttattta aacaacattt cttgtatttt ttgccacttc cacaagggca
    27421 tagctcattt ctccctaaca atatgacttc cactccctta ttattaaatt aaagtacaat
    27481 ttgttattat accatttttt tattaatcaa tctatagtat aagtaaatat tctattaaat
    27541 tcacttatat tttggtacaa tatattaact ttaattagga tttcgataaa atcatcaaca
    27601 atccctcttg tagaaatgaa ttatcgtaaa actaattcct ctataaaatt ttattaattg
    27661 ataatagttt tttactttat ctattctttt ttgttatgtg actctaactg tcattttaca
    27721 ttcaacaata ttgtcttttt cttgtttata ttcactgaga atattttgag catcaaaatt
    27781 atttactttt aatttgttaa tttctatctt ttcaagttta ttatttagcc ttaatttctt
    27841 cattacatct tcaaatttca acacattttc aagttctcca ttaaccttta tttctaaatt
    27901 aacttgtttt ttgttatgat tatctaaact actttcttga ttaatatatt ctatgttcac
    27961 aatatttttc aattcatttt ctaccaataa cactatatct ttatttgttt ttttatctaa
    28021 aaatgagtta ttaaaattat tttttaattc ttcattttca tttaattcaa cactttctat
    28081 atatttatca tctatatgct tattaccctt tatttgataa ttatcttgat tatttatttt
    28141 atttattata ttttgcttct cattcttcaa actagttaag ttttcttcca agtaatttac
    28201 cactatatca cttgccacca taataactaa tattaatagt attttaagta gatttttttc
    28261 tctattattt aatctcaaaa ataatcaccc atctaacttt ttattctaaa tctataatat
    28321 tctccatctc ttacaatact atctatgtaa taatatttta tgtactttat tttccttaaa
    28381 ttgtctaaaa catctaaatc actacacaca ccttctatct ctaatttatt tttatctatc
    28441 catattctag taatactatt tattccaatt gtttcatata ttttttttat ttcattaagt
    28501 tttattttag aaaatttttc tctagtttta tctgttgttg atatagtgtg ctttgattta
    28561 tcaactaact tattttgact tatataatct tcaagttcat ttatagattt cacatacata
    28621 aatgatttaa ataaaataca tgttaaaatt aagactatta ttattttata gactttatta
    28681 catgatttag tttttttgaa tccattaaga aaatttattg ttttatctac tttcacttaa
    28741 ataatcattc ctatgctact tatataattt atactattgt cttctaaatc tgtatcaaaa
    28801 ttattttcta cttttacttt atcttttaat cttaatggat ttacttcaat agatttatct
    28861 gtatatttaa agtattcttc aataaaacaa ttttctatac catagtaata aatatacttg
    28921 aagtccctta ataatctttt tataaactca tatgattttc tctcttttga tagcaagtat
    28981 gtttcttcta tttttttatc ttttgttata tttataatca actgactttt tttacattct
    29041 ataaatactc cttcttctga aaagttctct attaaattta agtttataag cttttgtaat
    29101 atatcaaaat ttatatttat agtctttgga ttcatattta gattttctga aatgttctta
    29161 attaattgaa taatactctt tggaaaaaga attacttgta tattcaattt atcatttata
    29221 gaatttataa ctttatattt tattgaatag ttacttatgt ttattggaat gtattgtgtt
    29281 atttcaaatt caatcatgct taatatatct ctttttttct tagtatctag aacttcaaca
    29341 tttctaatga ctatatcttg aatttgaatg ttaaagtata aatttttata tgatattttt
    29401 gatttatcaa tctcctcttt tacaaatcta actacatctt ctatatattt aatcttctca
    29461 atcgaattac tatcattttt tatattaaag ttaatctctt ttataattga tttatctata
    29521 aaaacttttt tatgactttt tcttaaaaat acaagcctta tataatcatc atttatttct
    29581 aaatttagaa tttttttaat tgtagccatc tccttcttaa ataaaaataa attttaaaac
    29641 ttacaacaat caatataaaa tattattttg gataaaattg cttagaattt atattcacgc
    29701 ttatatctgt tccgttatca tttataacaa gttcgaattt acctgatgta ctctgaggaa
    29761 ctggaacagt atttaggtat cccttttcct tcaatgcttc tacacttaga gagtctatta
    29821 tattttcatc atcattaatt gccatactag ctgctgttac tatattagca gcattcgtat
    29881 agtctgcatt taatttagca ctttcttgaa ccttactata cttaactaat gctatactag
    29941 atataactcc taaaatagtt actactacaa tcatttcaac aagtgtaaat ccttttcgtt
    30001 ttttatttat caattaaata ttctctcctt ttcaaaagtt tcatctaata aaatttattt
    30061 ttctttctaa aaactagtaa ttgcatcaaa cataggaaca accattgcca aaacaaaaat
    30121 gccaataatt aatcccataa agacaattag cacaggctct ataaatttca ttatttgctc
    30181 tattttgtta tttaattcat tttcataaaa tttatttatt acatctaggc ttttatctag
    30241 tttgccactt tcttctcctg tatttgtcat tgaaataaat aatttcggaa aaatatttgc
    30301 taaattcaag gatttaccta tgctatttcc tttttttata tagttattag aaatttttag
    30361 cctttcataa ataaattcat tgtctataac actagctgac atatctatag cttccacaat
    30421 ttgtattcca ctactaatta aaatggacaa cgcccttgaa aatcttgttg tagctactaa
    30481 ttgattaatt ctttttacaa atggtacttt aaattttaat gaattagtta atttttttat
    30541 attatcattt gttataaaga aataaatact tcctattgaa aacaatataa aactaaatat
    30601 cagataagct aaattatttg taaccactgt agatattccc attaaaactc tagttattaa
    30661 aggtggttca attccattac tggtaaaaac cacctgaaaa ttaggtatta caaatacgaa
    30721 tataaacaac attgataaga tagataatat tataagtatt attggataaa ttgaaatagt
    30781 tattattttt atttttaatt tgtattcttt atcataataa ttagataaat cgctcattat
    30841 tatatctaaa ttcccactag tttctcctgc ttttatcata cttataaaga atttagaaaa
    30901 tatacctgtt atttgaaatg attctgctat tgagttacct ttttgaatat gatttgatac
    30961 tatattaagc aaattcttta cttttttact tgactgactc tcaatgattt caaatatctt
    31021 tgtaatttca catccagatt ccatcagtat gcctatctct ttgcaaatta ctctaatttc
    31081 tttatcactg attctattta atactaaata aaacttttct aaattacaaa atatattttt
    31141 agataatctc atatactata ataccttcca aattatatta attacaatta aataaactta
    31201 atattttcta atttaataca tttaatttta ttttacttga atataattta tactcaaaca
    31261 tcttaattaa aatagattaa ataaatacaa ttgagcaaac gaaatttaac tattcaattc
    31321 agtcaaatca tttcatttac tactaaacac tcttctaatg ttgttatttt ttcatttatt
    31381 agccttttac aactatcttc aaatgttatc attccattat tttgtgccac gtctttaata
    31441 gtactagaat cctccatatt tcttatacat gtctttatat catcattaat ttctaaaatt
    31501 tcataaattg ctgttcttcc aaagtatcct ttatcattac atttttcaca acctacagct
    31561 actttagtat ttacatctcc actaaaatca tcttttatca taatctcatg actacaatga
    31621 ttacaaactt ttctaaccaa cttttgagat ataacaccta taagagatgc gtttaaaaga
    31681 tatggtggta tttgcatttc tagtaatcta gctatggaag atactgcatc attagtatgt
    31741 aaagtactta taactaaatg acctgttata gctgctctta ctgctatttt agctgtttct
    31801 gcatctctta tttctccgac cattattata tctgggtctt gtcttaatat cgctctaaga
    31861 cctacatcaa agctcaaccc tacttttgaa tttacttgaa tctgacttat gccatccatc
    31921 ttatactcta caggattttc tatagtcatt atattcttat ttattccttt taaatcattt
    31981 aataaggaat atactgttgt agtctttcca cttcctgtag gacctgtaac caataatatt
    32041 cctgctcttt tatttattat acgatttata gattttatag cttcctctga aaaaccaagt
    32101 tctagcttgt cttttaaaaa agtatctcta ttaagtatcc tcaaaactat tttttcacca
    32161 taaacagttg gtattgatga aactctaatg tcaattaaat ttccacccaa ctttatatct
    32221 accctaccat cctgaggaag ccttttttct gttatatcca ttgatgcctt taattttaca
    32281 accgtagaca atgagggata gacatcaact aaaaactttg atacttcctt tagttctcca
    32341 tctaccctca ttctcactat taaatgttct ttaaatggtt ctatatgtat atcacttgca
    32401 ttttctttta tagctttttc taaaagattt tcaaataatt tttttgcata gtcctcgtca
    32461 agttcaaagc tattgtaata tttttcaatc ttttcactta taagttcttt cttctctttc
    32521 ttcaatataa tttctttacc tgtagctaat ctcaagtctt gtagagcata aatatcctca
    32581 tcttctattt ctacaactaa attatcatct tgaaattcaa ctggaaatac attatatttt
    32641 tttgcaagct tttctggtat taacattggc aaactgtttt cctctaatat aatcttaatc
    32701 accctttata tactttaatt tattaggtat attcattttt ttcatagaga tattttcttc
    32761 agtttttatg cttattttca ttggataatt ggtttgtatg ctatctaaat atttactacc
    32821 tttatctaca taattagata gtttctcctt atctccaatg cactttattt catatggctg
    32881 tgctacagga actccattta tatttatatg gcttcctgca aggacaactt ctgaatattg
    32941 attaattctt tgaccattta tttctatgta ttttcctcca tttaatttta actcatttat
    33001 gacttttaca agaatcttat tatagtctat actattagct atattacctg ttttttcatc
    33061 tatagcatct atacttaata ttatcccact acccttcata tctgaatatg atagactatc
    33121 tctaagcgtt tctatttgtg atattttttc agaatttttt tgattttttt tcattgaatc
    33181 aatttcttta tctaacttgc ctttctcttt ggttaattct tttatagatt ttcttgtcat
    33241 ttttatttct tttcttattg acttatcttt gcttgtataa actgtttctt cattgctata
    33301 atctctaaat atggttccaa caagtatacc taaaatagta cttgctagta ttataaacac
    33361 atattgtttt ttcatactgt attgctcctt acaaatttat ttttatgtga taattttctt
    33421 taattaaata ttaaacattt acatacgttt attatatcaa agttatatat taaaattcac
    33481 ctttagtata taatatcata tattgaactc ctatcactaa cttaccaata tcaagtatgt
    33541 atttttaaat gttattaaaa ttatataaac tttcaataaa cattgttgta atattataaa
    33601 aaacataaat tagaaaagga gataataatc atgaaaaaac aaacaataac cttaggatta
    33661 acaagtataa tagatttatg aggaattggt ttacttaggc aaatcatttg cagataaaaa
    33721 tactgttaaa aaaaatttca actaactatt ctggatatga catactagaa aaatgaaatt
    33781 caactatttc tactggtttt agttcaaagt aaattcatca tacgcttata ttttcaatca
    33841 aattatcttt ttttatataa caatttatga ttattaataa ttattttcta tcaaaaaagt
    33901 aaaatggcat agccttaaag ctacaccaat ttactaaaaa ttatatcata attaacatta
    33961 atgttaaaca tatgcgttta taagttttcc tcatatttta aaattctttt aaccaagtta
    34021 tctcttaatt ttcctacttc atcaaatctc atattaggac tatagtaact ttctataata
    34081 tcatgtttag atttagctct cttaagattt aacactgccc aacaaattaa atcatctaaa
    34141 actttttcat caaatttaat atcttcttca tatttaagta gtttttctct atttaaaaac
    34201 tgatttaaat caatcgtatc ttcttcttta aaattcgtgc ttatagttat acctatatct
    34261 aagtcagtta atagtatagt ttcaattttc tctggaataa gtggataatg gtaaacttct
    34321 acatttaaac ctttttcaat agctttatta tacactttgt ttaatagtgt agttttccca
    34381 gttcctattt ctccagctaa ataataaacc ttatttacat cttttaaaat gttttctgta
    34441 taatctaaac accctacagg agttatagct gaaccaaata aatgcttttc tctttttaga
    34501 tttcctgtgc ttttaactcc atcaaataac ctttttataa attcttctgt gactaaattt
    34561 actttaccat aatccataca atcagaatat ttttcttcta tgtctctaaa gattggttct
    34621 gccgatttta aaaacttaaa tgctctctta aatgaattac taatatcttt atcgtagact
    34681 gatatttctt gtctattctt ttctaattct tctgtattcc aataatcacc tagattaatt
    34741 atctcgtcca aagctccagg tagctttggg tccatagtat gtggagcagt tgcatccata
    34801 agtaccactc ctaatttctt tataactaag ccatccaacg aacttgggtc agaaggacaa
    34861 tgatataaat caatatcata tcctcgatgc aaaaactctt tataaatttt tttcataagt
    34921 gaggattttc ctactccagg tccacccttt aaacaaaaaa ctctttttac attttcagga
    34981 attatatagt caaaaaaaga atatgagcct tgtgatgtat ttccacctgg gaatgccttc
    35041 tttacctttc cttccatatt agcgcctcct cataatttac aatatctctt aattaagata
    35101 ttcatattat aagaatttta ttacagcttt ctgattttga caaatttaaa acttgctatc
    35161 taatactaat aacaggttca ttttatctaa ttattgtaga ttcttatgct atgccttttt
    35221 atctataaat gcaatttaag tgtccatatc taagtatttg tattgtaata tagctctata
    35281 ttacttaaat ccaattgttt ccattataat tttatgttaa aataatatta caaaatacat
    35341 ctgtttttct tcataaacgg gtgaaattcc ctatcggcgg taaaagcccg cgagccttat
    35401 ggcataattt ggtcaaattc caaagccgac agtaaaatct ggatggtaga agaaaatagt
    35461 atatgagtac ctttatgtaa ttttacatga gtaatctata caaatccttc aactaccgta
    35521 tttattcatg aaattagaca cattcaaggt acctaatata caggtgcttt ttttgttgtt
    35581 tattttataa ttatatcgta cttataaaat ctattaagat tggagtgtta tcatgaaaca
    35641 aaaatggata gtattgatta tcatctgtat tggtgtattt atgtctactc ttgatggaag
    35701 tatactaaat atagcaaatc ctacaattgc tgctgacttt aaaattaaca tgtcacaaat
    35761 tcagtgggtt gtaacagctt atatgcttgt tgtaacagct actatgttat ttttcggaaa
    35821 acttggcgat aaagtaggct caaatcgtct atacacatta ggatttttta tatttacaat
    35881 aggttctttt ctgtgtagta tgtcaaacaa tttatcaaca ttaatttctt caagaatttt
    35941 tcaggcagtt ggagcaagta ttttaatggc taccggactt ggtatagttt ccaatgcatt
    36001 tccaacaaac gaaaaaggaa aagctatcgg aatcactgga gctgtagttg gtataggaaa
    36061 tatgagtgga cctgtaattg gaggtataat tttagaacat tttggatggc cttctatttt
    36121 tattatcaat ataccaattg gtattatagc agtctttctt ggtattaaat ttcttcctaa
    36181 gcctgtttta gatgagcaaa gcaaaagctt tgatattcca ggtcttctac ttttcgcatc
    36241 ttgcactacg cttatattac ttgcaatgaa tgagaaaggt aatacaagat tgtatttagg
    36301 tataatagca cttataatct tcttgctatt ggcattgaga gaagtaaaat ttgaacaaag
    36361 ttttattgac ctacctctat ttaaaaatag aaactttact gtaggcaata tcattggagt
    36421 agcttgttat tttcctcaaa tggcagtatc ttttctactt ccattttatt tagaacaatt
    36481 aaaaaattta tctcctatga tggctggttc tgtaatgact gtacatccac ttattatggt
    36541 acttatagca cctattgcag gttctctttc tgataaacat ggtgcaaaaa atattctaac
    36601 agcatctttt tcatttatga ccatctcact agttggaatg gcacttttaa aagcagattc
    36661 tccactgtat cttttaattg tatgtttagt tatttttgga cttgggctag gagcgtttag
    36721 ttcaccaaac aatagtagta ttttagcaga tgttcctcct caaaaacagg gatatggagg
    36781 cagttttctt gcaactgttc gtaatttgtc atttgctctt ggaacagcat tttttagcag
    36841 tttttttgca caaagtctta catacaatca aaagttcaaa agtcacacat cagcctatgt
    36901 tatagctagc aataaatctt attggattgc agcatcagta tgttttatag ggcttatact
    36961 aactgttttc tttatgagaa aaacagataa atctatttct taaataaaat ttatttctga
    37021 tttcatcagt gattaaattc ttaaatatac taatttatat caaactttac agtttttttc
    37081 taatttataa caataaattg ctaatattta ttgtttataa acaacaagga tgccaaaaaa
    37141 tgtccattaa aactattttg aggcatccta tattttattt tgatattaaa acagttagaa
    37201 ctaatctaat gttattttat atagattatt taaacaagta ttacgccaac atttgatagt
    37261 ttaaatttaa tccactgtta tctcatatcc atgttgatta tattgattta caatttcttc
    37321 ccatagttct tcaacaccag tcttttttag tgaagaaaca ggaattactt tttcgctact
    37381 ttccatttgc aatttttttc ttattatact tatatgtttt tgatattgac ctctagatat
    37441 tttatctgcc ttagttgcaa taactacaca gttatatcca aagtgcttta tccattcata
    37501 cataagctta tcatctgctg taggttcatg tctaatatct acaagtagga atatagaaca
    37561 taactcttgt ctactttcta gatatctttc cattatacct ccccactttt ctttttcaga
    37621 cttagctatc tttgcatatc catagcctgg aaggtctaca aaataaaatt cattatttat
    37681 taaataaaaa tttatagttc tagtttttcc tggtgtttga ctagttctag caaaatttct
    37741 tctgttaagc aatgtattta ttattgatga ttttccaaca tttgacctac cagccaatgc
    37801 tatctctggg attccttctg ctggatactg tgatttattc acagcactca tggttatctc
    37861 tgaacttcta attttcatta ttttcaccac tctttaataa agcttgttct aatacttcat
    37921 ccatatgctc tacaagcaca aactccattt tttcttttac attttctggt atttcatcta
    37981 agtcagcttc acactcttta ggaattaata cttttgttat accagctcta tgagctgcta
    38041 ataacttttc tttaactcct cctactgcta atactctacc tcttaaagtt atctctcctg
    38101 tcattgctat atttccaggc acaggtcttt tagtaagtgc tgagattact gcaagagcca
    38161 tagttatacc agctgatggt ccatcttttg gaacagcacc ctctggaatg tgtatatgaa
    38221 tatcattcgt cttataaaaa tcagggtcta tatcaaactt gtccactatt gaccttatat
    38281 aagagatacc tgtcttagca gattccttca taacatctcc tagctttcca gttaatacaa
    38341 tttctccctt acctttaagt acatttactt ctacttcaag tgtaactcca ccaactgctg
    38401 tccatgcaag accatttact aatcctattt gagggttaac ttctgcaagt tgatacttaa
    38461 acatatcttt tccaagatat gcctctaagt cagacttact tataacaact tcttcaagag
    38521 ttgggtcttc aacgtatttt ttggcaactt ttctacaaat cttaccaata gttctttcta
    38581 gtgttcttac accagcctcc cttgtataat gattaattat acttcttaaa gtttcatcat
    38641 ctatttttat aaagttttct tttaaagcat gttctttaat ttgttttgga agtaggtatt
    38701 tttttgctat atttaatttt tcttcttcta tatatccaga tacttcgatt acttccattc
    38761 tatctaataa tggtcttggt atattaccta ggctatttgc tgttgtaaca aatagtattt
    38821 ttgataaatc aaaaggtatt tctaagtaat ggtccacaaa atccttgttt tgctctgggt
    38881 ctaatacttc tagcattgct gaagctgggt cacctttata atctgctgcc atcttatcta
    38941 tctcatcaaa taagaacaca ggattttttg tttgagcttc ttttacccca ttaattattc
    39001 ttcctggaat tgaacctaca taagttcttc tatgacctct tatttctgct tcatctctaa
    39061 ctccacctaa agatattctt acaaatttcc tatttaatgc tctggcaatt gattttacta
    39121 tagatgtttt tcctgttcct ggaggaccaa ctaaacatat tataggacct ttaagtgact
    39181 tagcaagagt tcttatagct agatactcta gtattctctc cttaactttt tctaatccat
    39241 aatgatcttc atctaatata tcttttgcct tagttatatc taatttatct tttgtctctt
    39301 tattccatgg taatgagaat atagtatcta aatagtttct gcttacagat acatctggag
    39361 acattgaaga tatttttgaa aatttatcta tttctttttc tattttttct ttagttgtct
    39421 taggtgcttt tattttttta agcttttctc tatactcatc tgcttcagag tttatatctt
    39481 cttcttcacc taattctttc tgtatagctt ttaattgttc tctaagataa tattcttttt
    39541 gaactttatt catctgtttt tttactctta aagttatttt cttttctatt tttaatatat
    39601 ctatttcctc taataatata gaataaataa gttcaagtct ttttctaata tcaaattctt
    39661 ctaatatttc ttgtttttgg cttgatttta gatatatgtt tgcagctatc gtatctataa
    39721 atctatctac atcttctata tcagctaaag atattaatat ttctggtgat accctattcc
    39781 caatatttat atactcttca aaggcatcaa atacatttct tacaaatgct tcaatttcaa
    39841 cttctgtctc actatccaaa ttatcactat caaacactat ttcttctatt actgctctga
    39901 aatatccatc ttcttgttct attttcttta ccttacctct tgatacacct tcaactaaaa
    39961 cacgaactgt atctcctggt aattttatca tttgcttaac tttacagatt gttcccacat
    40021 gataaaaatc ctcttctcct ggctcatcta cctctgcttc tttctgagaa gttaaaaata
    40081 taagttcttc atccatcata gcttggtcta aagctttaag ggatatctct ctacctatat
    40141 caaaatttaa tatcatatat ggaaatatag ctaatcctct tagaggaatt aatggtaatt
    40201 catgatctat tttagtataa ttttgttcca tcaacattac tccttttcta gtctaattgc
    40261 tttgctttta atattatata tttgaacaag ttaattttca acattaatga taaattctta
    40321 gaatactatc atactatatt atctttttat tatgatataa tattaatatt agtatactta
    40381 gatgttattt atcttttgga ggtttctatt atgttgaaat tgtttttaac ctttttaaaa
    40441 attggtgcgt ttacatttgg tggtggatac gctatggttc ctcttataca agatgaactt
    40501 gtaaacaaac aaaaactttt agagcaggaa gaatttattg attatttatc catagctcaa
    40561 agttatcctg gagtactggc agttaatata tctgtattat taggatataa actttatggt
    40621 ataccaggag ttcttatatg taccttaggc tctgctttac catcattttg tatagtctta
    40681 gtcttatcgt atctatactt taaaaattca tcatctaaaa ttttagatgg attttttaaa
    40741 ggagttgcgc ctgttgtaat agctcttata ttgtattctt ttacgagcat gttcaagaaa
    40801 ctaccaaagt ctaaaactaa catactacta ttaattatag cagttttatc tgttggtgta
    40861 ttcaatataa gccctattta tttaataatt ggaggaggtg tatatagtct atgtcagaaa
    40921 taatactact cttcttaata ttccttaaaa taggggcatt tagttttggt ggcggttatg
    40981 ccatgctacc atttatccag caagaattta tatctaaata tcactttata actaatcaag
    41041 agtttttgga tttacttgct atatcacaat ctacaccagg tcctgtagca ataaatagtg
    41101 ccacatttgt aggatttaag actgctggct tctttggttc tctagctgca actgttggtg
    41161 ttacaatttt tgcattaata ggtctaacta ttatatcaaa gttattttct aagtttaaaa
    41221 acaataaaaa agtagaaaac ttattgacag ttcttagacc aattactcta ggattcatat
    41281 tatcagcagc tttttcatct gccaaagaag ttaactggga tttatatgga ataattgcat
    41341 ttatagcatc attttatctt ttatataaag gaaaaattgg ttccattgca attgtattcg
    41401 tctttggtat tctaggtata ttactcacat ttttataata atgataccta ttatatttag
    41461 catattagca taaaaaaagg agcttttaaa gctccttttc atcatcttat gcactttcct
    41521 cttgatcctt aagtactatg ataggattta ctgagtcttc gttcacagat ttctctgtta
    41581 ctataacctt ctttatatta tctctagatg gtacttcaaa catagtttcc atcattacac
    41641 tttcaactat acttctaagt cctctagctc ctgtatttct ttctatggct tttttagcga
    41701 ttgcacgaag agcaccttct tcaaattcta gctcaacatc atctaactcc aataactttt
    41761 tatactgttt tactagagca tttttaggtt cctgtaatat ctgcattaat gcatcttcat
    41821 ccaacaactc taaagtagct aaaactggta ttcttcctat aaactctggt attataccat
    41881 attttaataa atcctctgga agtacttttt catacaactt ccctaaatct aattcttttt
    41941 tactttctat tttagctcca aaacctagtg ttttatctcc accacgtttt tgaattatct
    42001 tctctaatcc atcaaaagct ccacctaata taaataatac attagtagta tctatcttta
    42061 aaaattcttg atgaggatgt tttcttcctc cttgtggtgg aacatttgca acagtacctt
    42121 ccaatatttt taaaagtgcc tgttgaacac cttcaccact aacatctcta gttatagatg
    42181 ggttttcaga ttttcttgca atcttatcta tctcatctat atatattata cctctttcag
    42241 ctttttctat atcaaaatca gcagcttgaa taagttttaa aagtatattt tcaacgtctt
    42301 ctccaacata tccagcttca gtcaatgatg tagcatctgc cattgcaaaa ggtacattta
    42361 gagttcttgc taaagtttga gcaagcaatg tcttcccaga acctgtaggt cctaataaaa
    42421 gtatattact cttttgaatt tctatatctt tgctactaga ttttttacta tatattctct
    42481 tgtaatgatt ataaactgca actgataaag cttttttagc tttttcttga cctattacat
    42541 aatcatttag aatctccatc atttcctttg gttttggtaa agatgtagta tcctcatcta
    42601 tagtatcttc tatctcttct tgaataattt cgtcacaaag ttcaacacac tcgtcacaaa
    42661 tatatacatt tggacctgca attaatcttc ttacttgatc ttgattttta ccgcagaatg
    42721 aacactttaa ttgtcgtttt tcttcatatt tagacatatt aacacctctc tctatctacg
    42781 gtctctttgt aaatacttca tctattaaac catattcttt ggcttctagt gcactcatga
    42841 agttatcacg ttcagtatcc attttaattt tttctaatgg ctgaccagtt ctttcagata
    42901 atatctcatt taaagtttct tttattttta aaattctctt tgcatgaatt tctatatctg
    42961 ttgcttgacc ttgagctcca cctaatggtt gatgtatcat tatttcacta tttggtagtg
    43021 ctagtctttt tccttttgct cctgctgcca acaagaatgc tcccatagaa gcagccatac
    43081 ctatacatat agtacttaca tctggtttga tgtattgcat agtatcatat atagccattc
    43141 cagaagttat acttcctcct ggagaattta tatataaatg tatatctttg tctgggtctt
    43201 cagcttctaa aaataatagc tgtgctacta taagccctgc tgtggcatcg ttaacttgat
    43261 ctcctaagaa gattattctg tcttttaata gtctagaaaa aatatcataa gatctttctc
    43321 ctcttcctgt ttgttcaaca actacaggta ctaatgccat ggtttcatac cccctttatt
    43381 ctatataatt tacagtagct ttttttaagc tactgtaaat tattatacgc atttaactaa
    43441 attatatttg cacatcatta tatactaaac atttcataac gtcaactata ttagttagcg
    43501 tttattttat taagctattg ttgcattatc aactagtaaa tctattgttt ttcttatttt
    43561 tatttgacct tttatatctt ctttgtcagc atctcttaaa gaagcttcta ttttttctac
    43621 ttccatattg taagcagaag ccattttttc taactcagcc ttaaattctt cttctgttgc
    43681 ttcaattcct tcagctttag ttattgcttc taatactaaa gaagatttaa ctaatttttt
    43741 agcatcttct tttctctctt ctcttaattc ttccatagtt ctaccagtca tctctaataa
    43801 ttgttgtaat ccaaatcctt gatattgtaa ttggtagtta agttcattta acatattatc
    43861 tatttgatgt tgtatcataa cttctggtat ttctatttct gtattttcag caactttttc
    43921 tactactgaa tttctagttt ctgcatctgc tctattttta gcttcttctt ctaacttagc
    43981 tcttacatct gcttttaatt catctaaaga atcaaattca ctagtatctt tagcaaattc
    44041 atcatcaagt gcagataatt cttttacttt tacatcgttt atttttacat taaatacaac
    44101 tggttttcca gctaaatctt gtgagtgata ttcttctggg aaagtaacat taacttctac
    44161 ttcttcacca gcttttttac ctactaattg ctcttcaaat cctggtataa atgtgtttga
    44221 acctataact aagttataat tttcaccttt accaccatca aaagctacac cattttcaaa
    44281 accttcgaag tctattatag ctgtatctcc atcttctaaa gctttatctt ctacacttac
    44341 taatctagca tttttttcaa ccatttcttg tagtttagca tcaacatttt cgtcacttac
    44401 agtattctca accttagcta tttctatacc cttgtagttt cctaattcaa actctggttt
    44461 aacttctaca ttaagaacca taaccaaccc attgtcttta cttatttcct ctatatctaa
    44521 atctggattg tctattgggt ctatgtctaa ttccttgata gcttctggat aaacttctgg
    44581 gaataatatt tctatagcat cattatagaa tatacctttt ccatattgag tttctataac
    44641 aattcttgga gctttacctt ttctaaatcc aggtatattg tatttatttc tgcttttatt
    44701 ataagcctta tttactgctg cttcaaattt attgttatct actgttattt ttaaagtaac
    44761 tttattaccc tcttttttaa ttagttctgc tttcatcctt ctaatcctcc tagtgatatt
    44821 tatgtaatac ccgataacgg tattataatt cattctattt ataattcaac cacttaatta
    44881 tatcacataa gtttttttat ttacaagtgt tccattaata tcgttgtact tatttgataa
    44941 aatttaattt tttgagcgtt ttttgccatt tttttagata aatacacctt tatatgcgta
    45001 tttcttctat atttatgtta tcttcttcta tatccataat gacaaaactt ttatacgata
    45061 ctcctcttgg aagtgaagta cttccaggat ttatgtaaag tacaccatct ttaatttctc
    45121 taaacggagt atgggtatga ccaaatataa ctatatcagc atcaatttca gttgcctttt
    45181 tttctaacat atgcgttcca tattttacac catacttatc tccatgacat aagaatatag
    45241 ttttatttgc cacttcaaag acaacttctt cctcaacagc atcaaagtca caattacctt
    45301 taactgcaat aatacctaca ttagtcatgc tatgaatata tcttgaatct gtaaagttat
    45361 ctcctgcatg aattattagg tcacattctt ttaaatatgg tattgttttt tccatatttt
    45421 taatcatcat atgtgtatcg cttactatgc ctattttcat tacttcacta ccctttttat
    45481 aaactcttgt ttcataagtt ttagtgcatt tgctctatga ctgattgaat tttttataac
    45541 actaggtatt tcaccaaaag tcttatcata tccttttact ataaataaac tatcatatcc
    45601 aaaaccattt ttaccttttt cttcaaatcc tatcattcct tcacaaattc ctcttactac
    45661 aaattctttt ccatcaggaa aaactacggc tattgcagag acgaatcttg catttctttt
    45721 actcattggt acattttgca tagcttttaa cagttttttt ctattttctt catccgttgc
    45781 atgttctcct gcatatctag ctgaataaac accaggtttt ttgcctaaag catctacttc
    45841 taagccagaa tcatctgata ttgctataag tttagtcttt tttgctattg ttctagcttt
    45901 tatcaacgca ttatgctcaa atgtttttcc atcttctact atatctatac ctgctaaatc
    45961 aacatctttt aaagagtata ctttatactc aaaatctttt aagatttcac ctatttcttc
    46021 tagcttatga gcattattag tagcaatcac tacttcatta ccataatcca tacccaatat
    46081 ttcatctgct atttcaccta aagcttcttt ttgaagcctt attaattctt tattcccttt
    46141 ttcacctaac tctaacaata aatttaaatc tgttctcgaa aaaggtttct cttctccagt
    46201 cccctgtact tctacaaact cacacttatc agtcattatt atattcatat ctacctgtgc
    46261 tttagagtct tcttcatagc ataaatctag catgtgtgtt ccatttacaa taccaacact
    46321 aattgcagat acaaaatttg ttattggcat ttcttttact gtctttgctt tatataattt
    46381 atatattgct tctgcaactg ctacaaatgc ccctgttata gatgcagttc tagttccacc
    46441 atctgcttga attacatcac aatctaccca aatagttctt tcccctaatt tattcaagtc
    46501 tacaacagac cttattgctc ttcctattaa tctttgtatt tcttgtgttc ttccatcaat
    46561 ttttccccta gatgcatctc ttatctttct ttgttgtgtt gctcttggta gcatagaata
    46621 ttcagcattt atccatccag tgccactatt tcttaaaaaa ggaggaactt tttcttccac
    46681 tgatgcagtg catataacct ttgtctctcc catttctata agaacagaac cttctgcata
    46741 ctttgtataa tctctagtta tctttacagg tcttatctgc tcgttacttc tatcataact
    46801 tctggtcatt ttttatatcc tttcttatat ttttgttttt tataaattta tttttataat
    46861 ttttaatttt gtcaaatatt aattattgtc atttttcgta atcttattag cttactgaac
    46921 tattctatca ttaattatag tagttttcta ctaaattaag cgacttttta actctctaat
    46981 atagctttta cttcatcttt cgatgataac tttaatagtt ctaaaaatct ctcttcatct
    47041 ataactttaa ttcctaaatc atttgcttta gcgagtttag aaccagcttc ttcacctgcc
    47101 aaaacaaatg ttgtcgattt actaacacta gatgtagctt ttccacctct tttttctatc
    47161 atatcttttg catcatttct ttttaatgta ggaagtgtac cagtcaatac gattttcatc
    47221 ccttcaaata ttttttctac tttttcacct gtattatcca atgattttgt atttactcct
    47281 acatttttta acttgtttat aacgttcata tttttgtctt ccttaaaaaa ctcgacaacg
    47341 ctgtttgcca ttatacttcc aaattcctct aattcttcta gttgttcact cgttgcagac
    47401 atcacatcat ctaaatcctt aaagttatat gcaagtatct tagctgcttt aactcctata
    47461 aatttaatac ctagtccatt tataagtctc cataaatcat tttcttttga tttttcaatt
    47521 gaatttatta aattgtcagc agatttttcg cccattctct ctagctcgac tacatcttcc
    47581 ttttttatat aatataaatc agatacatct tgaataattt tttcatttaa tagcagtgta
    47641 attatagatt ctcccaatcc atctatattc attgcatccc ttgatacaaa gtgtataatt
    47701 ccccttctta tttgagctgg acaagacatg tttatacatt ttacagcagc ttctccttct
    47761 aatcttattg ttggctctga acaaactgga cacttttctg gcattttaaa atctatttca
    47821 tctccagtac gttcttcttt tataacttct aaaacttgag gtattatatc ccctgctttt
    47881 tgaactaata ctgtatcatt tatttttata tccttttctc ttatataatc ttcattatga
    47941 agtgtagccc tgcttacagt agtacctgca agtctgactg gttcaagaat tgctgaaggt
    48001 gttattgtac cagttctccc aacttctact attatatcta ctattttagt tttcttcttt
    48061 tctgctggga atttatatgc aattgcccat ctaggacttt tggctgtata tcccatttcg
    48121 tctctttgtt ttatattgtt tacttttatt accataccat ctatttcgta ttctaaatta
    48181 cccctgttat ctgtccaata ttcaatgtgt tcaataactt catctatact tttacaaact
    48241 ttataactct gacttactga aaatcctagt ttttctaagt actctagcga gtcactgtga
    48301 ctttctaaat cgtaatcttc tatattttcc atattaaata caaatatatc taatggtctc
    48361 tttgcagtta gttttgggtc taattgtctc aatgaaccag ctgcaagatt ccttggattg
    48421 gcaaataatg gctgttcttg ttcttcttgt tgttcattta cctttctaaa attatctttt
    48481 gatatatata cttctcctct aactattaac tcttttttat catttatctt aagaggtatg
    48541 ctttttactg tcattaggtt ttgagaaata tcttctccta caactccatc tcctctagtt
    48601 gctccttttt caaactctcc attattataa gttagaccta cagaaagacc atctatttta
    48661 aattcaacaa catattcaac attttctcca acatattctt gaaccctctt atcaaaatcc
    48721 ctcaaatctt gctctgaaaa agcatttgat aaactcaaca taggagtttt atgtacaatt
    48781 tgattgaatt tatctaatgg cttacctcct accctatttg ttggcgaatc aattctcttc
    48841 aattctggat gttcttcttc taaagaaatt aactccttca ttaacttatc atattcataa
    48901 tctgatattt ctgggctatc ttgattataa tacctttcat tatggtaatt tattgtctct
    48961 ataagtttat taatttcatc ttttatgctc actttttgca cctcttcata ttttttaaat
    49021 taaattattt tgaaatttcc ttataattaa ttatctccta tttatatatt ttttagtata
    49081 tcactttttt gactttaaat ttattacaaa aaaaactctt attagtatat ctaatacgac
    49141 taataagagt tttttatttt tatcaaaatt ttttattttt agtatcctct tgatatgtct
    49201 acaacgttat ctacaggttt attttccagt atatagtgtt tcaagttatt ataaaccata
    49261 ttaaatgttc tctctttgtt tttatctgac acccaagaat tatgaggagt tactatgaca
    49321 ttttcacact cccataactt attatccttg tttaaaggtt cactttcaaa tacgtctaat
    49381 gctacccctc taaatttttc tatacattct tctaaatctt tctgattaac tgtatttccc
    49441 ctacctacat tgataaatac agaaccatct ttcataagtt taaacatatc tttatttact
    49501 attccatctg tgctttttgt agaaggcatt gctacaacta ctatatcaca ttcttcaaat
    49561 acaatattca tatcctcagt ggcaaaacac ttatcaaagt attttttact acttcccgca
    49621 gtatttacac cccatatttc tagaccaaaa gcttttaatc tttttgctgc ttctgaagat
    49681 atagtacctg ttcctaaaaa tccaactctt tgtccataca attccgatac agaaaaatcc
    49741 atcttccatt ctttattttg ttgttgttta aaaaattttg cactattttt atatatttct
    49801 aaaatataca atacaatgct ttcaccaata ggtacactat atccgcctct attattagct
    49861 atcttaatat ttctcttcaa tacttcttct tttggaagtt ggtctatacc tacacttgtt
    49921 gtctgtatat attgtaaatt tttcatctta gagatatcta atctctcaaa tggattgtat
    49981 gtaactagta catctgctgt atttacatcc tcattatttt caataatatc ttcagaataa
    50041 tgtatgacat catagcctaa atctcttatt ttatcaaatt tttcttttcc ataatttatt
    50101 gtaaataaaa ctttcatctc aaatctcctc catctactca cattaaaaat tttatattct
    50161 aaagtataat atgatactca atgcacatta gttaaaatta ctagaagata tcttaaattt
    50221 acttatgttc aattgttttt cctaaagaca tagcattacg ttcttgaact aaattttcta
    50281 ttaaattatt caatgtccaa tcaatagatg tctctgttac catagctgta aaaggtctat
    50341 ttatcctcac tttttttagt ccttctaaaa atgcatttac ctgtgtactt ggagcgttat
    50401 taaatattac agacttactc ttaatcccat tttcacattc acttgatata tttccatcta
    50461 aaatgtctct tattatatta tctccatttt tatcacttaa gatgatatgg tctcttattc
    50521 cagcaaagtt acttacattt ttaattaatg ataattcttt tttattaaaa tttacaagca
    50581 taatacactt cctattgtgt attttatcta ggttattatt atttattttt tcaaatgaca
    50641 ttttatccat cctttcattt atttatatat tttaatttac cccatacatt taaaataatc
    50701 aatttttata gttaattttt atattaatac ccaaattata tttaaatagt tagactatcc
    50761 aaaactaata tataaaatta gaataattag attaatatct acaaatcata acctaaagga
    50821 gagataccat gaatacatta ctaaacaata aatttagcat taaaaatttt tcaaatgctt
    50881 gggttatagt gcttggttgt atgttgatac aagctgttcc atttagtatt gtagcaagta
    50941 tacaacctca atttataagt tatgttgtag aaggagaagg ctttagttta gcttcttttt
    51001 ctctaatatt cactattggt actattgcct cagcaattgc atcacctttt ataggtgtat
    51061 tattttctaa agtcaatatt aagatactat atttattagg ttgtataatt tctagtattg
    51121 cttttgctac tttttctata tgtactgaac tatggcaatt ttacacggta tcatgtattg
    51181 ttcaaattgg tgctacaatt atatctgcta taggaatacc tgttttaata agtgcttggt
    51241 ttgatgaaga aagcaaagga aaagctctag gctttgcttt tgctggtggt tctattggta
    51301 gtatatttct acaaccactt gcagttcgct tactatcaac tcaaggatat agtcgttctt
    51361 atctaatatt tggaattgta gcattaatag ttggtatacc tgtaatgtat atcaggtgac
    51421 accgtatgat atgattggaa cgccattcta atgcggtatc tacgggattt gttgaaaagt
    51481 caaagtatcg taaaatgatg tcaaatattg ggctgtatcg caaaattggg agtacaaagg
    51541 gagtacagaa aaaatcctat gggagtacaa aaagtatgat ataaactgtc ttttggaaca
    51601 atattatgag ggcgtattgt gtaagcgata tgctctttaa tttttgagaa aagtaatgct
    51661 acagactagg aaatatgata gaatgacaga aataaatagg aattgcagta ttgagaatga
    51721 ggaggtttag tttatgcaat atttattgat aattatcata ggacttttgc tttttattga
    51781 agttgataag ttgaaaaaac aggtaaaatc acaacaaaag cagattgata ctttgtgtga
    51841 acagacgggg aataatcaac tggcaacgta tcatatatcg gatgaggata aagaacttat
    51901 tcttcatttg aaaaatagtg gaaaagatgt agaggctgtg aaaaaagtaa gagagattac
    51961 tgctatggaa ttagaagaag caaaacaata tgtagactct ctttaagagt tcaattttct
    52021 gatatagtaa aaagtaagat ttggataatg gaggtgttca aatggataaa agacattggt
    52081 tcttacttgg ttatttaatt attccagtat gcttttttgt tgcagttatt gtagttggtt
    52141 tgctaagaag tcattcttta attgaaattt ataatgacgg attaggaatt acggcattat
    52201 attacctttt acttagtctg tttgtatata ttcgctggca atatttttct gacaaataaa
    52261 gaatttagga ggttaagaaa tgtatattat ttatacagtg ttagccgcta tagtgggtat
    52321 ggctttggta gttaccgttg atatgggaat aacaaagata tttgataaaa agagcatttt
    52381 tcaaaaaaat tatattaaga tgccactttc ttatttcatt gggatttttg tttttctttt
    52441 gattgtgaga ttgatttttt cataaagaaa tagagttgta aattcagctt tgtagcagac
    52501 agttttaatg taactgtctg cttttccttt ttaatcataa ttgataatag tgctataaaa
    52561 tggttcatat ccattgtgaa aagaccattt agagtgatat agattgatta taagagtagt
    52621 aggagtcccg ttgatttgat tatgttctgc ttcgatttcc gctaccactt catcggtatt
    52681 ccagtattga tatacgataa cttgcaattc ggtatcacga ttagccccat tgctgaatga
    52741 catactgtta aatacaagat aatcgggcaa gctaatgtaa tagagaatgc ctgcaagcaa
    52801 gactagaata atagtggcaa ataacaattt ctttttcatt ttgacatacc tcagtttcta
    52861 tatagttata gggataacta tatagtacct cgctatatag tgaattgcta cataaagatt
    52921 tattgaatat acttttatgt agaggtgagg caatgaagta tggacatttg gaacttcaca
    52981 tagaggaact gctgaaagaa aaaggaatta gtaagaatac catttgtaag gaattggata
    53041 taccgaggtc taactttaat cgttattgca gaaatgaatt tcagagatta gacgcacaac
    53101 taatctgtaa actgtgcagt tacttggatt gtggaataga agaattgata gagtatgtga
    53161 aagaatgaga gcctgtcgga ttgaagaaaa ttcgtgcagg cttttgttgt gtcaagaaaa
    53221 agagaaaacg tagttaggtc aaggaacaag gcgaagccga tacgccgtag ggcagtcctt
    53281 gacggaacgg acatagccaa cagcaccatg atacagtaga ggacttgaaa ggtcagaaac
    53341 cttgcaagtc ctcttgtctg ttactggtcg ttatgtttta agtaaagtgc ttctttcatc
    53401 agttcttttt caaatgcctt gatgtgaccg ccctgtattc cgttgttgat atgaaagcaa
    53461 tcgtcagcct gtgagtagga tagcatggta actttcggct cataggtctt ggtatcaatt
    53521 ccataatagt aaatcgtggc ggttgttaat accatgtgaa tgctttcaaa atctcttgta
    53581 aaaattgtat gcctgtcgga atagaagaaa tcagacggac attcatagaa ttgggaacta
    53641 tcctctttca aaaatagtgg cagtatgtgc attgcatttc cagcacgatt gacaaggaca
    53701 taaaagcgtg gaatgagggc ggcatattgt gtcatcatgg cgatatatat atcatctaaa
    53761 tcatcaagaa gatactcaga gcaggagttg ttataaaaga catattgcag ggcgttatcg
    53821 ttttgacttg tatgttccat aggcacattt tgtccgtaaa gccaacgcaa gtccacatct
    53881 aaagagtcgg caatcagttc cattttatcg gctttcggat tgtactgtcc tgaaagatag
    53941 gaactgaacg ctcctttgct gataccagta tctttgacta tatctacttg ttttttattt
    54001 tttgctttca tggcaaattt aatccgttct gcgattgtat tcatctgaaa accctctctt
    54061 tccatatccc gttataactt ttcatatctt atcgtctaag gacactatac cacaagttca
    54121 gaaaaaagaa aataaaaagt ttataaaaaa ctaaactttg tgttgcaaaa caaatatgtc
    54181 tgtgctaata tacaattaag tttagaaata actgaactaa aacgacgtcg taataggaaa
    54241 atgaaaatat gagaaactga cctatcggtt caacggggag aaaggaacgt gtatgcagat
    54301 tacattgaca aaagaacagg taaaagaagc atttggagaa cttcgtttta tgggtgcgga
    54361 taactgttac cgctatgata aagacagtaa gaagaaaaca gaggaagtgg aagcattaaa
    54421 acttcatctt ggaagcatga agcttgggaa cagcgtggat attcgcttgg aagcgacgga
    54481 acttccaaag attgagcctt atgctgtggt ggaactggaa gaatcagtat atgcccctta
    54541 tgtgcagaga ggaaacttcc ctgtattagt ggagaagatt acctgtaagg gtatccgccc
    54601 tgttccaaac aaaaatatgt aggcggtcaa agttcctgcc ctcgttctga cgtaccaaac
    54661 ggcaggttga gggcagggca atggcaacgc cattgcagat aggaggtatg ggaaatttgg
    54721 aagaaagaag cggaagtttt cttccagaac tcccctatac taacagggga gtaatacgac
    54781 agaaagaagc attaagtgcc ttgatagact ggtgtcagat aaccattaag gaagttccgt
    54841 tagaagcagt tatagaagat gtgctgagaa tacctttgga acttatgacg gtaacaggct
    54901 atgaaaaggg gattgcagga catgaggttg tggcaatctt cgataatatc aaagtgctaa
    54961 agccgacagg aaatgcacag tatcaaggct ttcagatttt gatgagtggg aaaggttgtc
    55021 gcaactatga aaatttttta caactcaatg aggaaacatg gtttgatttt ttaaacagag
    55081 tttgtcagta ccacatcaat gttcctcgga ttgatttggc gatcgacgac agaaaaccgt
    55141 atttgagtat tcccgatttg attgtacgga caaaagaggg attgctttcc acaaaactaa
    55201 gggagataga ttttcacgac tcgggagagc tgaaagagga agtgtttcag agtaagggtg
    55261 gtagtcttta tctcggcagt tcagccagca atttgagatt ggtattttat gaaaaaggat
    55321 atgaacagaa taaaaaatat ggaacagaat tagacgaaaa ttggaacaga tatgagttga
    55381 gatttcggca ggaaatggca gtgagtgtag tacaggaatt attgagatat agagatgtgg
    55441 caggactggc aatggaagta ttgaacagca aaatgcgatt tttagaaaag ccgacagaca
    55501 gtatgacaac acgaaaaaga ctttatccaa cttatcaagc atgggcggaa ctgatgaaag
    55561 acataggcaa ggtcaaactc accatgcaac cacagaagaa aagtttacaa aaggtgtgga
    55621 agtggctaga aaagtatgtt gccccgtctt tgaagttgtt tgcagaggtt gggaagatag
    55681 agcagagaga ttatatcggt aatctagtga aaaatggaga aatgaatatc gcacagaagc
    55741 agttgtatga tgattatata aaagcaagaa aattagacga aagggggtaa tcgtatagaa
    55801 aaggtattat tgaatttaaa agaggtaagt gagtatcttg gaattggaga aacaaaaaca
    55861 agagaacttt tgagggatag aaattgtccg ttttccatga agattggcta tcggtggtat
    55921 gcgaataaaa aaatattaga taaatgggta caagaacaat cagagaaaaa agggggaaat
    55981 tattctattt gatatgtatg taagtaagtg gtatagtgta agagataaaa tgttctataa
    56041 agatatttat atcatacaga aaggtgtgtg atataaattg ggtaagagtt taaaaggaaa
    56101 agaattaggt gcaggactgt atcaaagaaa agacggaaaa tattgtggtc gtttctatga
    56161 ccgctttggt attagacgtt atgtatatgg aaaaaccatt tctgaaacaa aagagaagtt
    56221 aaatacagca atgtatgaag ataaaatgga aatgaatgtt gccgataata ctgtgacatt
    56281 agatgaatgg tatcagaaat ggttggaaat atacaaatac aaagtgattg cacccagtac
    56341 aagagtacaa tatgagagca tttataaaaa gcatatacaa acagatttcg gacacatgaa
    56401 actgaaagat atacagcctt tacaaatcag agggaaatta gtaaatcttg ataaaaaggg
    56461 atttggatat gaaactcaga ataaagtacg tattttactg ttagatatgt ttaataaagc
    56521 ggttgcggac aacgtggcaa tgaaaaatcc tgtaaggaat attaaagttg ttcgagatga
    56581 agaaaaagaa cctcgtgtac tgtcaacaga agaacaatct gccttttttg acacctgtaa
    56641 aggaacattt tacgataatc tttttaatgt ggcaatatca acaggacttc gccccggcga
    56701 attatatgct ttagaagctg aggacttgga ttttgagaaa aaggtaatac atattacaaa
    56761 atcgttatcc tatcagaaat tcgagggaga tacaggaaag acttttcaca tgggaaaaac
    56821 gaaaacgaag tccagttatc gtgatgtgcc tatgaataga agatgtgaaa ttgcattaaa
    56881 gaaacagtta ttgcagaaaa gagttgtttc gggtagaaat ggtgcaaaaa cattaaaagg
    56941 atttgaaaat ttgattttta caacaaaata tgatactcct cttaatgcac agatttatgc
    57001 agatgctatc aaggtaatcg ttgagcaaat caatcttatg cgagaggaat tggaacaatt
    57061 tgaaacattt tcggggcatt gctttagaca tacatttgca acaagatgtt ttgaagcagg
    57121 aatacagccg aaaacagttc agaaatatct aggtcatgct acattacaaa tgacaatgga
    57181 tttatacacc catgtgctag aaaaccattt atcagatgaa atggataagt tagatagcag
    57241 gctggaaatg attgaacttt caagtgatga tttagcagag caacgttttg aacgggaata
    57301 taggaaacag aacgaagttt tgtcctctga taagttaata cgcataggat aaaagggagt
    57361 ataataggag taacagaaaa tgctatggga gtacaaagtg cttgaaaatg gcttaaataa
    57421 aggaaaaatc aagatatgat ttgtactccc tagttgtttt attatattat tcatacttag
    57481 gatgcctaaa gatgttaatg aaaaagtaaa atctaaaggt aaaaaagaga aagcatctga
    57541 tacaatttct ccttccgtat ggggttatac attaaaagaa gctaaaaaaa ttaaatattt
    57601 ttggattatg acgattggat ttatatttgt tggtatatat gtttcgtcat tatctgtaca
    57661 atatccaact tatcttagaa attatctaaa tcctattcta ataggaacag taggctcgac
    57721 atttgcttta tttgctctca ttggtaattt atttggagga ttattgtttg ataaattcgg
    57781 tcaaataaag ccacttatta tatcttttgt attggttctt atagcaactt tatcattact
    57841 attggtagat aaatcttctg cttttgcatt tgtatttgcc attttgaaag gtctttctgt
    57901 atttgcttat atgattggtc ctgcttattt aactggcact ttctttggtc aaaaggaatt
    57961 tggtagcata tatggtatcg tacaattaat ttttgcagtg ggatttgcat taggttcaac
    58021 attatttgga ttactagttg ataattttgg atatagattt tcttggtcta ttataatttt
    58081 atcagttata attgcctata ctttacttat tataacttct ataggaatga aaaaattgaa
    58141 taaagaaaga ctagataaac tgatgcaata atcgattttt agatatataa atattatatg
    58201 aaaaagatgg agcttttaat cataaataaa actccatctt tttatattac aatatagcta
    58261 attttctctt atttttaact ttactatcta tatcctcatc cttaactata gtaaatgcct
    58321 tagttggaca aacatttaca catgcaggtc cttctggtga ttctatacaa aaatcacact
    58381 tatttgctgt cattctttct ctaaaacaaa gctttccttc atctacatct tttaatgtaa
    58441 ctatgtctat tgcacctata ggacatgcta acatacaagt cttacatcct atacaatttt
    58501 cttcatttat ttttattgtg ttcccaaccc taactatacc accatttgga catgcctttg
    58561 cacaaggagc atcttcacaa tgtctacact gtattggagc acttacttct tttgtcttta
    58621 tgacagaaag ttttggatta aaatttattt catctggatt ttggtaaaaa atatcttcat
    58681 ctgaatgtgc aactacacat cctatcatac aagttctaca tcctatgcat ttatcagggt
    58741 ctgctataac aaaatagttc atcacttagc cacttccttt cctatacaat atgtagtgtg
    58801 taatagatga tgagattttt ctcctaatgg ttctaccaaa tattcatcat atagtttttt
    58861 aatatcaggg ttctcatgag attttctaat tgctaaatgt tcatcatgca catacgttgc
    58921 ttttgttcta ttttcatagg ccaattcctt atattcttct agtaaaattt ttggctgacc
    58981 tccaccgctt acacacccaa caggacaagt cataacttca ataaaatcta catctaattt
    59041 tcctttttcc aagtcttcaa gcactggtat tacatttttt aatccactta ctactccaac
    59101 tcttaagtct aagtctccta ttttaatagt tgactttcta aaaccatttt ctcctcttac
    59161 ttgttttact tctacatcag gaatactttc tccagttata agctcatatc ctgttcttat
    59221 agcagcttcc ataactccac cagtaacacc aaagatagtt ccagctccac tataactacc
    59281 taaagggcta tcaaattttt cttctcttaa atttttgaaa tcaattccca tgtcttttat
    59341 taaatacgct agttctctag tagttaacac tacatctaca tctctatatc cactagaatc
    59401 catctcttct ctatcacatt catatttttt acatgtacat ggcattatag aaacactaaa
    59461 tatctttgat gcatctacat tatttatctc tgcaccataa gttttaaata ttgcaccacc
    59521 catctgctga ggtgatttac aagaagataa atgattagtt aatttaggat aatttttttc
    59581 catgaatttt acccaagctg gacaacaaga tgtaaacatt ggaagagttc caccttctgt
    59641 caccctttta attaactcac taccttcttc cattatagtt aaatctgctg caaaattagt
    59701 atcgtaaact ctatcaaaat ttaaagctct aagtgctgct gccattttcc cagcacctaa
    59761 gtatcctaaa taatatccaa aatcttcccc taaagcaact ctaactgctg gtgcacactg
    59821 aaccatagta aatacatttg ggtcacttaa agctttttta accttatcta tatcacatac
    59881 attgtatgct gcaaatatag gttcatttat agactctggt atctctcttt cttgtttttt
    59941 ctcttgcaaa aactcaaatc cttcatctat aactgaggca taagatttac aagtttgaac
    60001 acattgacca cacataacac atctatctaa atttatctct tgtggctttc cctcttcacc
    60061 ttcaatagca tttactggac agacttttga acattctcta catccagtac ataaatcttt
    60121 atctatattt attatcataa acgtcaccta cccctgtatc ctttatttat ttgttaacaa
    60181 taagctaagt gcagcttgtc tattcttagc ttttttgtca tctattggag taacaagttt
    60241 taatgcttcc tgaggacatg cacttataca agctattttt tcactgtctt tacataagtc
    60301 acatttataa gctatctttt tattttcatc taaaattact tgttctactt ctttaccatc
    60361 ttgatattgt ggaagtaaat ctatagctcc aaaaggacat gctaacagac aagttttaca
    60421 tcctatgcac ttttcttcat ttacagacat aatattattt tctttgatta tagctttttg
    60481 tggacatgag tttaaacaag gagcatcttc acagtgtcta cactgtattg gcatacaaaa
    60541 attgtcacct tttactaaat aaagtctagg tataacaggt atatcaactg ttcctactgt
    60601 ataaccaaca ttattatgct gattatgcat tgtaaaacaa gctacttcac aagctctaca
    60661 acctgtacat ttgtttgggt ctgctattac aaaagagcct attttatttt tcatattatc
    60721 ccacctttca atttgtatat ttccaaccct ctagttacct tcatcatata tatttatata
    60781 ttttaatatt ttctcaatac tcatatttcg tttacatttt ttgcacaaat tgtatgtgtt
    60841 gttatacacc ttatcagtat cattaattaa tactccgcag atttcacact ttgaatattt
    60901 tagctcttct tttttagtac acatatactc attctcactt tttgataact ctctgtggat
    60961 ttgcataaat attcatccgt cttcctctta caaatccaat aagggttata tttaattttt
    61021 tagaaagctc tattgataaa ttagtcggtg ctgattttga tactacaata ggaatttgag
    61081 tcatagctgc ctttaatatc atttccaatg atattcttcc acttacagct acaattttat
    61141 cttttaatgc tatatcattt aaaacacaaa atccaatagc cttatccatt gcattatgcc
    61201 ttgctacatc ttcacaagta actattcttt tatcaaaatt aaatatggat acactatgta
    61261 ctccaccggt atttttaaat aactctgatg aatttaaatt tctatccatt atactgtaaa
    61321 tcgtatcata atttattttt atattgctct ctacaggagc acatgttata tagtctagtg
    61381 aatttaaaaa tataatttgc tcattttgat ttttttgctt ctcatgaata gttacttttg
    61441 caaaacgttc actctcattt atctcaaatt ttaataagct ttttttattt tctatatatc
    61501 cttttgtttt caaaaatcct actataagct cctctaaatt agatggtgta cataaaaatg
    61561 tatttatata tttattattt aaaataaaac taagtggata ttctgcaatt atttcttcat
    61621 tatctattga tacttcatgt tcatttattt tacttacttc tacattaaaa gaatttaaat
    61681 atttgtgctc cactagacta taactatttt caaaaacttt atccttacta ttttgttgta
    61741 atttagtttt tgagtatata ttgttcataa aatattagcc tttctgaatt ttataaatct
    61801 ttaatttaat aaatcattag atatttgtag ctgtcatttt atctttcaaa cttttatact
    61861 cttctcttac acatttctct gctaattctt ggtcttcaag tttttcaagc ttaacagcac
    61921 aatatttata ctctggtgtt ttagatatag ggtctaaatt tgctattgta agttcattac
    61981 aagccccaac ccaccactga taagtcatat atgttgcacc ttccttaact ctatcagtaa
    62041 ctgttgctct tgttattaca cttcctcttc ttgaactaat tctaactaat tcatcatctt
    62101 caattcctaa ttttttagca tcattagaat ttatttgaac acgccctggt tcatcttcta
    62161 atgaactaag tgttctgcaa ttaccagtca ttgttctcac tgaatagtgt ccaacttctc
    62221 ttacagtaca caaactaaat ggatactcat catcttcaac ttccatagga ggtctccatt
    62281 cagcagcaaa caaattacct ttaccattag gagtagaaaa cttttgacct tcatatagat
    62341 acatagtacc tttatcttcc atgctttcgc tcttacaagg ccattgaaca cacccttgag
    62401 cttctatctt ttcataagta gcacctaaga aacttggaca taattgtcta agttcatccc
    62461 atatttcctt agtattttta taattcatag gatatcccat tgctgtagaa atttcgctta
    62521 taatttgcca atctggtttt acatcacctt gtggctctat agcctttctc atcaactgga
    62581 atcctctatc tgcacatgta tatactccct catgttcccc ccaagaagta gctggaagta
    62641 taacatcagc atgaagtgct gtcttattca taaatatatc ttgaacaatg acaaactcca
    62701 attcatctaa agcttctcta acttctgatg catctgggtc actttgcact gggtcttctc
    62761 caaatatata ataagcttta agctttttct cttttagaac taaatctgga acttgagtta
    62821 aactataacc attattagga gatagtttta ctccccaggc tttttcaaac ttttctctta
    62881 tcttatcatc agttacattt tgatagccag gatatacatt aggaagagct cccatatcac
    62941 aagctccttg aacattattt tgtccacgta caggtcctat tccaacgctt tctctaccaa
    63001 aattacctgt aagtagagca attgatgcaa gtcccttaac tacatcaaca gcttgaccaa
    63061 attgacagac acccattcca taaagtatca tagctttttt gccttttgca tattccctca
    63121 tagcttttct tataagctct tctgggattc ctgtaatctt ttcagcatat ttagctgtat
    63181 atggtttaac aatttcttta tactcatcaa atccttgtgt atgattttga acaaattctt
    63241 tattgtaaag accttcttct attaagacat ttccaaaagc atttactaaa accatgtttg
    63301 ttccaccttt tataggtaga tgtatatcag caattcttgc agattcagtt actctagggt
    63361 cagtaacaat taatttagca ccattttttt tagcttttac tattctattt gccactattg
    63421 gatgtgaatc tgctccatta tatccaaata taaataaaac gtctgcattt tctatctctg
    63481 gtatagaatt agacattgca ccactaccta atgagtaagc cagaccggct acagatggtg
    63541 catgtcagac acgagcacaa tggtcaacat tattagttcc tattgtagct ctcataaatt
    63601 tttgcattat ataatttgct tcattaccag gtcctcttgc agaacccgtt cccataatag
    63661 aatcaggtcc atatttttct ttaatttcat ttaatcttga agctgcatag tttatagcct
    63721 catcccattc aacctcttct agtacacctt gttttcttat cattggcttt tttagcctag
    63781 gtgttagaat tttagggtca tttaggaaat cccatccata acgacctttt aaacacaaac
    63841 tttcttgatt tgttctacca ttagctggtt cagctcttaa tatcttatta tcttttacta
    63901 ctagatataa ttggcatcca gctccacagt atggacaaac tgttaaaatt tttttctcca
    63961 tacaattccc cccttagatt tatcccctga taagtaaaat ttatcttaaa gataatgagt
    64021 gtttttataa tatgcaaaat cataaatatt ttgcaatact atctattaaa ttcaacaatt
    64081 ttctcacaat tccttttaaa tttcaaaaaa aatatgtcat aataatcttt atatttcaac
    64141 catttaatta aaatattcta gttcttatga catatttttt aatatactca atatatatta
    64201 tttaaactta tttagttttc acttaaaaca atatttctgc caattctatt tttcattcta
    64261 tagtgagtaa ttacagtaaa tattccactt ccaatcagaa tcaaatagta cataaatcca
    64321 ccatataaaa ataccgcata tcccatcaaa ggttgaggga atactgattt aaatatagta
    64381 aaaaatgcta attcattagc tcctacattt ccaggtgttg gtattggaga aattgccata
    64441 tataaaaatg cttgtaatgt aagtatatag atatagctat aaccttgtaa attaaatgct
    64501 ttatatatcc agaatgaaac actaaagtaa gcacttaatt gaataaaagt tactattata
    64561 gttgaaatta atactttttt attctttgca aaaaaattta ttgctttact ataatcatct
    64621 ataaaacttt ctatagattc aatcttatta tctaaaaatt ttaaaaactt aatttttgat
    64681 aaatgtttta tagtaattct cataaaatat tttatttttt gaggatttaa aataactaat
    64741 attatcataa ttaagacaaa tgaattaatg gctatcccta gaaacactag aggcataatc
    64801 attttcattt gtttttctaa catagcaaaa ttcattaata caagtattgt acaataaaaa
    64861 gtaaccacta tttggaaaat aatagactta ttggtaacaa ctgcacttgc cttactaagt
    64921 ggcattttac attttttaag tacatatatc tgtactggtt ggcttcctga agcaaaagga
    64981 gttattaaat tataataaaa tcccataatg gctagcttaa agccaataaa tctaatatta
    65041 actttgtgag tgctttctat aattatcttc attactactc cttcaagcat tatgtgacac
    65101 attattgcca aagcacctat gaataaaaac ttcttatcta ccataaccat aacatttgat
    65161 agcattttaa tatccaatgt tttaaacact aggtaaattg ttatacctat taacaaaaat
    65221 aaaaatgtat actgtagtaa actttttcta atactcttta gtctatccat tagaaataac
    65281 catcctttgt tcaagattta tactaaatac tatattatta gtatatacta tgaatcttaa
    65341 tattttataa caaaatacct tacaattttg tcatttacaa aaaatattaa tctatatatt
    65401 aaattttaat atatagatta atattttaca aacattttgt agatatatat aatattttca
    65461 ctaaacagca attatcttta ttacacccat ttatacccta tcccccatac agtttctatt
    65521 gtataaatat catattgttt taatttgctt cgtatatttt ttacatgctc tgtgatagta
    65581 cttatatcac tttctccatc aaaaccatat accgactcat atattctttc ttttgaaaat
    65641 acttgtccct tattttttgc taaaaattcg catatcagat attcactttt agtaaaatta
    65701 atttttttac catcaattaa aacttcttta cccaaaaagt taaattgaac atttgagata
    65761 gttaataaat tctttttttc tctactttct ctccttaaat gagcagttat tctagccctt
    65821 aattctccca caccaaaagg tttggttata taatcatctg ctcctatacc aagaccatac
    65881 ataatatcac tttccatatt tttagctgtc aaaaacagta ttggacaatc aaccttattt
    65941 cttatagctt tgcacacttc aaatccatcc atcttaggca tcattacatc tagtattatt
    66001 agattaaaag aattaaattc aatatccata gcattttcta tatcactaat cacctttact
    66061 tcatgtccat ctctttttaa tacattttca atcaacttta acacatctaa ctcatcatct
    66121 attaccagta ttttagacac actaactcct ccatttgcat gtttaataac attttaatac
    66181 ttatacttaa ttactttttt caattttata tgaatcatta tactctcata ttatatctta
    66241 caataactga ttgatgtttt acaaataaaa cattttagac tcaaaacttt atatgtaaaa
    66301 tattctatac ttattttata tatgtttatt taattataaa aatgtatttg ataatttatt
    66361 attgtaatat tttaaaaatg taataaaatc aataataaga aaacatctta taaacatatc
    66421 tttaattatt taaattggag gaaaaaatta tggatgaaaa gataagaagc agtaactata
    66481 atgttccatt tgactatgct agaaaaaaat ttaaagaatt agaccctcat acaattagta
    66541 atttgagtaa tatttctttc aacaatgatt caaacaagtt catactcaat ttctttgata
    66601 gacaatatat tatagactat ccaagtggag aagtttggaa tgatgatggt agtatctgta
    66661 aaaacttcga acttaaaatt ttaataatta gatacttaac taatgctaaa ggtataccta
    66721 gaactaataa atatgtgact tttaaggaaa taccaggagg aaatgtttac tatccaaact
    66781 ttttaaacag aacgctgtct agactttcgg aagtttttac atgtcaaata gacaaataca
    66841 agttggttat ggaaaatatg ggagctgaaa aagtagatat gggtgactta gcctataagt
    66901 ttacatatat gggtaataca tctatgatat ttatactttg gtttggagat gatgaattcc
    66961 ctccaaattc aaatatatta tttgattcta atataattta ttacttcaat gcagaagatt
    67021 tagcagttgt tcctgataca gctatagatg caattttaaa taaaataaat caattataaa
    67081 tataaataat attatcacac aggtatgata atggtgcaga ttattggact attaaaaaac
    67141 ctagaaaaat aatagttata tagataaaac atattaaaaa atagtggctt taaatttttt
    67201 aaagtcacta tttttttaaa atgatgttcc tgaaaatata ttaagttagt tattcta
//
LOCUS       DAFNNB010000004        62265 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0004, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000004 DAFNNB010000000
VERSION     DAFNNB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 62265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62265
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0004"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            306..935
                     /locus_tag="L3M60_000219"
     CDS             306..935
                     /locus_tag="L3M60_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224926.1"
                     /translation="MNKYLLINPVAGRMYGEKFHLVKENLMKKGYIIAECEPQLEYVK
                     KQYKEYTKKTENTMLDCRCPESINLLKRNNLINSFEVPMIEPILVRTCRVLYDKYIKS
                     KDDMLIVTCPCTQLRDFASEKFKDKSNAIFYTWKEFAEQEGVKSLGKIDESPIPLGYF
                     KDTFEKVLEVSSEDEILSEIQKIRNGSEDKYDIVEMFYCKDGCNNGDGL"
     gene            936..1718
                     /locus_tag="L3M60_000220"
     CDS             936..1718
                     /locus_tag="L3M60_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6224927.1"
                     /translation="MVNKFNNKFLRLVKSVLIFFIILLLWKITNYLGIWSDYILPSPE
                     KVYSTFLNMISDGSIFINVYASMKRVLIGFAISTAIGIPLGIFFGIYSGVYEYFKSLI
                     NFLRNTPPLALIPMLILWFGIGEESKIIIIVLASFFPIFTSTLKGIKNCDSKLIEVGR
                     VFEFSKLQIIFKIIIPNAILDIAVGLKLALGYSFRAIIGAELVAASSGLGYLISDGKE
                     MSRTDVVIVGIIVIGLLGIITDYIFSIIVKKVSKGKMVDAYE"
     gene            1711..2589
                     /locus_tag="L3M60_000221"
     CDS             1711..2589
                     /locus_tag="L3M60_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6224928.1"
                     /translation="MNNLVCDSGYIIRNLSKSYEVDNEEHVVLSDISLNIDRNHITVI
                     LGESGCGKTTLLRAIASLENINSGEIRFINDNKEYRPNVGFVFQESRLMPWLNVSENI
                     TFHNQRKNTILNKMLRKFKHNKRVIGKIINDNFRSLETNENLNKSNKYNRKSNIDVEK
                     YLKMMNLQKFKNSYPNELSGGMAQRVSIARALSFNPDMLFMDEPFSALDYFTRIDMQN
                     EVIRIHKSTNKGVIFVTHDIDEALKIGKKIVVFTDERKIKEFNIEDDYNRDLTSEYYI
                     KLKKDILMTMKSNKGI"
     gene            2622..3581
                     /locus_tag="L3M60_000222"
     CDS             2622..3581
                     /locus_tag="L3M60_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6224929.1"
                     /translation="MKLKKLLIGITILTLATASLAGCAKKNNGEKLSEINLTYVKSPL
                     NVPSIIQKQDDLFGKEFKKDNIKVNFHEITTGPEQTQALAAGEIDFLHALGGTSALIA
                     ASNGVELKILNTYSRSPKGFMILTNNNSIKSAADLVGKKVAGPKGTILHQVLISALDK
                     EGLSMDDVEFVNMGIPEASAALSDGSIDAALIAGPAALKAMKSGSKLVANGEGLVDGI
                     IVTAVSTDFAEKHPEIVERFMKVEKETLEYVNNNFDEAMEKVAKEVDLSLEETKELYV
                     WYDFSLDITDKDISSLEDTQDFLIKNKLQEKKVNIKELIYNAK"
     gene            3895..4587
                     /locus_tag="L3M60_000223"
     CDS             3895..4587
                     /locus_tag="L3M60_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6224930.1"
                     /translation="MSYKVYIVEDDISISTLLKDYIEKYGFEVIVEENFDDIMITFES
                     FKPHLVLLDVNLPKFDGFYWCRCIRQQSNLPIIFISARDGNVDQIRALESGADDYITK
                     PFYYDVVMAKIKSNIRRVYGEYSTKIDERIVELDGLKFYPERLELELHSKNLIITKKE
                     GILIECLIKRYPKVVSRDYLLEKIWDDIEFVEENTLNVNVSRIRKRFEELGIDNAVQT
                     IRGLGYKLNKNW"
     gene            4597..5601
                     /locus_tag="L3M60_000224"
     CDS             4597..5601
                     /locus_tag="L3M60_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6224931.1"
                     /translation="MKLFLKDYKGYIFIYYIGIVLTIIYCNLMKFISFDESLYILLFS
                     SFILLCFLIYKYYKNREVYNLFEKGIHSLEESVLYLGSSFLGRNISVILKQQHKLYQS
                     MIQEHKKIHGEHLTFINQWIHQMKTPISVISLQLQDYEGEEVTENIREEIDRLNKGLD
                     MAMHFARADNFQKDFIVEKAKLNEIVSSIVNEEKRLFIKSGMIPKIQIDKFTYVYTDV
                     KWMKFIIGQLVTNGVKYSKDCSNYITIKSEENNKYVILSIIDEGIGIVSKDLKRVFDP
                     FFTGENGRKFGESTGMGLYLVKKVCDDLGHKVSIKSEVGKGTEVSISFNKDVNSLIKM
                     "
     gene            5837..6688
                     /locus_tag="L3M60_000225"
     CDS             5837..6688
                     /locus_tag="L3M60_000225"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBN6224932.1"
                     /translation="MSLDVIFILKSVIIAIVEGLTEFIPVSSTGHMILVGNLIDFKGQ
                     FAEMFEVVIQLGAILAVVVLYWKKIKDSVREFLKFIFTGGKEGKIGFRFGMNVIIGCI
                     PFAIIGVLFYDNIKSLFNLQSVIIGFIVGGILLLVVETLFRKKNHSTDNIDKITPIQA
                     LKVGTLQVLSAWPGMSRSASTIMGGWIAGLNSPTAAEFSFFLAVPAMVASSGKDLFEF
                     DYSVMTPTLWIALVVGFIVAFIVSIIVMEKFVNFLKKKPMRVFAIYRIIMGAVLAVLA
                     LTNIISI"
     gene            6822..7568
                     /locus_tag="L3M60_000226"
     CDS             6822..7568
                     /locus_tag="L3M60_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001637630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6224933.1"
                     /translation="MKILTVNNLSKVYGKKIIFNALNDINFSIEDGEFVGIMGPSGSG
                     KTTLLNMISTIDKPTTGTMELKDKNLLLLKGEELALFRRRELGFVFQDFNLLDTLTIG
                     ENIVLPLTLDKVSVKEQDRRLNEVSEILGIKDLLGKRTFEVSGGQAQRTAIARALINR
                     PSILLADEPTGNLDSKSSKTVMELFQKINKENKVTTMMVTHDPLAASYCSRILFIKDG
                     AIYNEIYKGSSREQFYQDIMDVLTLLGGDN"
     gene            7568..9484
                     /locus_tag="L3M60_000227"
     CDS             7568..9484
                     /locus_tag="L3M60_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6224934.1"
                     /translation="MNIKQFAINNISRNIKAYLGYLASITISSSLLFSFVMFTSHPDL
                     DLSQFPDYLKEGLKMSKIIAYLFLFFFVFYSVSVFLKSRYKEFGILYITGISNRQVMK
                     LIFIENMIINIVSSVIGIIIGLIFAKIFLVVMSTFLELSPLKFYIPFNAILSTLIYFM
                     ILAVLTSLFTSFIVKENQVLKLLKGTKTPKPEPKTSLMLAILSICLFIIAYYNAVTST
                     DQYTTYLRLVPVTSLTIAATYLFFSQFSVFFIKKLKLNKRFYRKNTNMLWISNLIYKV
                     KDNARIFFLITITSAVAFTAIGTVYSFWKDVKRQINLIYPNTIYYSTMTLHNDPKKPD
                     SKEKDKERMRFIENNLKKEDIDYTRINGEFKTVFPKKSDFNVRIMKESKYLEITEVIG
                     VKPISFEDNESISLLSSSLPGNKKIKENVTVGNRSLKVAKQVEECVMPAYYKNMYVVK
                     DNFYDSIESKFIVDRFTAFEVEDSSKTIEICREFENKFDNESGMQPYLFFSKELMIET
                     GKLMYSTFIFLAIFIGLIFFVTTSSFLYNKFYMDCQVDKKKYEQLNKLGMTYNEIKKA
                     STIEIGIVFLLPYVVAVIHSIFALTALKNSFDLEVASSAVLVMGSLFLVQIVYFLIVR
                     NNYLKEIKLNLVNY"
     gene            9763..10680
                     /locus_tag="L3M60_000228"
     CDS             9763..10680
                     /locus_tag="L3M60_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6224935.1"
                     /translation="MMILIQSRGKMKCKELAEELEVSERQIKSYKMYLEQAGIFINST
                     PGIYGGYEIDKCNSISLIKLLDSEVSILDMINSQLEYNNDIYKTEFNSIVEKIKAVLN
                     TRVKSDTYMDYFTIQPQRNCDYKFEKNKCNEIIRAYTTKRKLWIEYYSLNSGNSERIV
                     HPYGLFNYKSDTYMVAFCEKRFKFIDFKLCRIKNYKVLEEKYNVDKSFSWDEYSKNSI
                     GIYKGEEINVVIKISHPFSTIIKEKVWVNNQQIIEYDDKSIMFKAKMRGYEEIKSWIL
                     SMGAYVEVVDPDSLRNDILSEIEKMKKIY"
     gene            10758..11495
                     /gene="cas6"
                     /locus_tag="L3M60_000229"
     CDS             10758..11495
                     /gene="cas6"
                     /locus_tag="L3M60_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBN6224936.1"
                     /translation="MKMKIEFSTSCIPISYNSLFMSIIKEAIKKSNEDYYKNMYYYKE
                     KNNKKTKNFTFSVYVKKYSIEGDNFIIEDKVILNISTPDLELGFHIYNGLMTSKKCLY
                     KDYELTRIRIDLSREKKVTEERVLFNALSPICVKSKEGKFLEITDDRYIEELNYITNE
                     VVKNYRGSGLKRKLEFENIDLKKVVVKESLREFKKITGKEYQYINGYKGKFILKGDID
                     DLNIIYNLGLGFRRAQGFGDVDILEWR"
     gene            11501..13351
                     /gene="cas8a1"
                     /locus_tag="L3M60_000230"
     CDS             11501..13351
                     /gene="cas8a1"
                     /locus_tag="L3M60_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas8b1/Cst1"
                     /protein_id="HBN6224937.1"
                     /translation="MKLRVYRGDWFFNMGIVGFLNIIKKSNKQAEIFIMEDYIEFDSS
                     FLENFHEYYFNYFMDEYDVSKRIKKSIDYSINFIKSKPDKVKDGIKKIKDTVKQQNDK
                     VKKFDEEKFNLIKEKLDSISKIKSYDEFDSLEILVDETIDIFKIKSINDRLTANLYKY
                     VVQDNYFGQVSFFNVAKAKLDLDGLKQVMFNDYLRQIIYFGELEDLLEENDYDKLKNY
                     LNDRLNSIAKDISEKKVSKSSVNTIEKIMKEINKNFIKKNKSIENIKEYMDSLEICEM
                     CGLYKGILDEYSESNFAPLGVSTNNARNMFWKQEYVSSICDVCKLILFCTPAGATYTR
                     KDYLTNEENEFYLFVNMDTSINELFDRNNSLKAQKSESSNSKDENPFNQLIKSIVEEN
                     KLKSEWQLRNILFVEFKASIDSKKCKINYFNIPTYLAKFFVKENKKIQSIYDYKLKSN
                     VLDLILKSKDLKHLINNTLRNIVKSNLESNNKSNISGLSCFNVVQLRTILNNYKRGVY
                     KMDYKKLRKIYESGCEIHDYYIDKNAKNKIEGITYKILNLIKVGNKNDFMQTILRIFS
                     PSEKTPPEEILQIFVEEDLDFESVGYAFVTGLISGKYEGKDKLSNEKEEK"
     gene            13353..14267
                     /gene="cas7i"
                     /locus_tag="L3M60_000231"
     CDS             13353..14267
                     /gene="cas7i"
                     /locus_tag="L3M60_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein
                     Cas7/Cst2/DevR"
                     /protein_id="HBN6224938.1"
                     /translation="MKRGLTFTVIAQAQSLNYGEGIGNISELKKLSRADGNIYTFASR
                     QCLRYDIVRLAAELFNWNLQVVDKEKGTIQFKDNISIRESEEMDLFGYMKTNKKDEKD
                     KKGGSLTREAAVRLSNAISLEPYRSDMDFLNNKGLADRIGEHPNLANIEQHLSYYTYT
                     VTIDLSKIGKDGEIELDNKEKCRRVVEFLEIIKVLNRNIRGRQENLSPLFVVGGVYEI
                     ANPFFLGRIKLKGDKNGFKINKQAIEEVVQGTFLGKYLKEFTYVGMVDGVFINKEEFK
                     GLFEDNFLSVDKFFGQLVKEVKEYYGVN"
     gene            14269..14970
                     /gene="cas5b"
                     /locus_tag="L3M60_000232"
     CDS             14269..14970
                     /gene="cas5b"
                     /locus_tag="L3M60_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBN6224939.1"
                     /translation="MKVLKLDLFQETACYKKPFAMKITETYPLPPYSTINGLIHKILN
                     ATEYIPFNISVQGTYESIFNNYQTTYFYKKDSITSMPMNSHMLLNVNLIIHIGGDFDL
                     LKEIYDSLLNYNEHLSLGRKEDLVRITDIRFVEVNKFEIDEDIQLDDYENYKLSECDI
                     RMPIYIPKTSLQDKDIDGISYRLNNYYDNNAVGDKKRVWNKVDSYYVEDGNTISVGDI
                     LLDNEGDLLYFNNIN"
     gene            14981..17302
                     /locus_tag="L3M60_000233"
     CDS             14981..17302
                     /locus_tag="L3M60_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBN6224940.1"
                     /translation="MLYAKSNPVETLREHTDELLKQMNVLRESYGTNINSLNFLEEEI
                     FWQLLDFVIEFHDIGKAFSPFQELIISRMDTKINEPKIVTHLENNVGHNYLSPAFIDY
                     SYIDKKKNKELRAVLNQVIVYHHERDIFIDKDFKNLIQKILDEDLINKVYELQHEFKV
                     RYPIKTEKLSKVYLQSVEKRIDKNHKYYNLYIMLKGILHRLDHSASAHEVVECNNVMN
                     IGEHTENYLMKEFGSLRETQSFAQSNRNKNIILIASTGMGKTETALIWIDKDKAFFTL
                     PLRVSINALFDRAKNIVGVGESNDTFLGLLHSTAIDYLEESNQENSSEIVDLAKLLSC
                     KLTFSTIDQIFKFPFLYRGYEKVYSTLAYSKVVIDEIQAYSPEIAAVLVKGIEMIHKI
                     GGKFMIMTATMPTIYIDELKKRGVVDSNLANLTCNTEKIRHCVSIVENSIDENLEQII
                     QSGINKKVLVIVNTVKSAIEKYELIEEIIKSKGIDINVNLLHSMYIQEDRAKLEKYIK
                     EFADSDSNGIWITTQLVEASLDIDFDELHTENSTLDSLFQRFGRCYRSREYEENSPNI
                     FIYTEKATGIGSIYDKDIVEKGLELLKNCMNGKKCFKMKEKHKVQMVKILYSKESLKD
                     TDFEKKFTRALDILDTITPYSLGSNEAQNILRNIEGYTVIPEDIYRKIEKTLIRDYEE
                     LGQELTEAYSNDDRQLINEIKSKRKKARREIIKKTVNLPIYKAKQNIVDIQVKGLEDL
                     KILLYKYDIHEDVHTKHIFGKGVIIGKEIDQFI"
     gene            17317..17868
                     /gene="cas4"
                     /locus_tag="L3M60_000234"
     CDS             17317..17868
                     /gene="cas4"
                     /locus_tag="L3M60_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBN6224941.1"
                     /translation="MWLKFTKEGEFVPIDLDLLKVQGVKINYYYVCKRKLWLFSKGIT
                     MEQNSSRVLSGKIVHEDSYKRMKNKEMLIDDILKLDIIKDEYVKEVKISSKMKEADEM
                     QLYYYLYYLKNSMGIEKKGTINYVKEKKQEELILTEEKERIIENTLININEINMKLSP
                     PKIEKKPYCTKCAYYEFCFVKEE"
     gene            17871..18851
                     /gene="cas1b"
                     /locus_tag="L3M60_000235"
     CDS             17871..18851
                     /gene="cas1b"
                     /locus_tag="L3M60_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBN6224942.1"
                     /translation="MYKDYYIISNGNIKRKENTIYYYNLEGDKKILPVEQVDDIHIYG
                     EVDLNTKLINYISQYGVMLHFYNYYGYYSGTYYPRKKNVSGFSLVCQSACYLDKNERL
                     YLAKSFVESASYHILRNMRYYKVDESLIDAIKDELNDLNDVKEISELMGKEGRIRKTY
                     YKSFNQILKDDFEFYKREKRPPKDPVNALMSFGNSIMYTNVLSEIYKTQLDPTISFLH
                     EPSTKRFSLSLDIAEIFKPIIIDTIIFSLINNKRINKNDFEYQDKICYLSEKGKKKFL
                     QEFENKMQTTIKHRNLNRKVSYRMFIRLECYKLIKHFINDERYKVLKVWW"
     gene            18854..19120
                     /gene="cas2"
                     /locus_tag="L3M60_000236"
     CDS             18854..19120
                     /gene="cas2"
                     /locus_tag="L3M60_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBN6224943.1"
                     /translation="MFVIVTYDIVEAKSLNRIRKILRKYLTWTQNSVFEGNITDGKLH
                     KCISEIENIIDNREDSIYIYEIKNPNSIKKKCYGIDKYSDEMFI"
     repeat_region   19304..21425
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=19304..19332
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaag"
     gene            21486..21914
                     /locus_tag="L3M60_000237"
     CDS             21486..21914
                     /locus_tag="L3M60_000237"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN6224944.1"
                     /translation="MIRKLNNKDINKIMEIWEESTIKAHGFISKEYWQNNHNAVKNEY
                     IPISDTFVYDIGDEIKGFISIIDKSFIGALFIKPKYQNLGIGGKLLDYATEKYKSLSL
                     AVYKDNKKAVIFYNKKGFNVVKGQLNEDSGFKEYIMEYSK"
     gene            22050..22217
                     /locus_tag="L3M60_000238"
     CDS             22050..22217
                     /locus_tag="L3M60_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224945.1"
                     /translation="MVTKEENKIVWCISNLTNLPFKNNKFDIILNVLSPVNYKRFERV
                     LNNYGMARKVC"
     gene            complement(22377..23147)
                     /locus_tag="L3M60_000239"
     CDS             complement(22377..23147)
                     /locus_tag="L3M60_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224946.1"
                     /translation="MKVNENNIDEELDELSRKVYLESPDFWISISDKIIDKVFDEMLL
                     FFAVKYNYFNIVCFAIENNYLDLNMPSKNKKYTNIMEHLLDTSKQNEDKKIYNYLLNL
                     NKDNNLDERPILDELNYNTSSDTFEKKDIYLPKYLCPSCNSNIFDTGYKVSNDITYKF
                     SSKEAEPIEISNEVSSIRCCNCDELLEDVTLDKLSNLCKIQNCNNCGSNLTKVGIIAK
                     KKMNFDENTEKFKDVSTSYCCGNCDSEINLSQREHFKI"
     gene            23471..24016
                     /locus_tag="L3M60_000240"
     CDS             23471..24016
                     /locus_tag="L3M60_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HBN6224947.1"
                     /translation="MKNTKVNIQDLTKMSICVALLCISSYIYIPLPFTPAGVTAQTIM
                     INLIALILTPRQAFSTVGVYIMIGLIGIPVFGGGTSGIGKLLSPTGGFYFGFLFAALI
                     ISLLKGRNNDIKRYILITIFVGMPIIYFMGTVFMCYFNQMTVEAALFAAVVPFLIGDT
                     IKSVIASFLGTKLNNVLRKNL"
     gene            24037..25173
                     /locus_tag="L3M60_000241"
     CDS             24037..25173
                     /locus_tag="L3M60_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HBN6224948.1"
                     /translation="MEEVFILGGLRSHIGLKNGIFQFVQPELLGASVLKHLIKKYEID
                     KIDEIICGNAVGTGGNIARLMTLTAGVSNEVPAFTVDMQCASAMMSIDIAFSKIKSGQ
                     CDLIIAGGFESSSLQPMRTYHKNDKRYNINNPNYTVAQFSPDDNSQNSMLEGAERVAE
                     LYQIKKAELDFWVKESHKRAKEAREEKIFDDIIFPINNSTCDEGIRDKMSQRLLDRMP
                     SILGKESITNAANSCLINDGASFIILCSKKYLEHAKNKPIAKIVNTCTIGTDANLSPT
                     SAIKAMDKLLEIESLSYLDVSAVEFNEAFAVIDVLFQRKYPELIDRYNIFGGALAYGH
                     PYGASGAIIALHLLKALEKTKGRYGICSIAAAGGLGSALMIERV"
     gene            25177..26568
                     /locus_tag="L3M60_000242"
     CDS             25177..26568
                     /locus_tag="L3M60_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HBN6224949.1"
                     /translation="MNYYELLRIKSDRHKNKNFLIIDGEKYTYENILSYSEEIGKQIY
                     LGENIPILIYSKNIMFQLISFFAINYSKNIPIICHYNLSKKVLDDILLKNNISIIISD
                     ESMDVIDLYDDNNNEVAKKVNLSIFNPKFNIYMYQYKKLINYLDKSICMGALSSGSTS
                     VPKVLYRTYESWAGFFPIQNAIFNISGESILFINGTLSFTGNLNSIMSVLYEGGSIVI
                     SSGLNCKSWIRTIKQYNVTNIYLIPIKLQLLVKHLKESVLKVVSIFTGSQLLFEDTAR
                     NLKKYMPKSEIILYYGASELNYITYLCYDELIERPLSVGRPFPGIDVYIREEKIFVNT
                     EYAVYNATKPYSVNDIGYFDNDGYLIFEGRSDDIVNIGGFKVSSTKIENEVKKIPQIE
                     DAVVLPYSDTIRGNQIVLFVITIDKITKKDLLIKMKDNLMKNEIPKKIIFLNSFPYTS
                     SEKIDRLALLKML"
     gene            26644..27519
                     /gene="dpsA"
                     /locus_tag="L3M60_000243"
     CDS             26644..27519
                     /gene="dpsA"
                     /locus_tag="L3M60_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HBN6224950.1"
                     /translation="MSNQYDITVIGGDLRQVYMVKKLINKGYSVVTYGISNEIINGIV
                     GKSTSLAEALSKSSIILCPIPFTKNQVNIENTDNCLDATIDNLLINLSPNQILFGGNI
                     PTKVCKFCEQHSIQIYDFMKMNDVAILNAVATAEGAISEAIKRSIINIHQSNCLVLGY
                     GRCAQILAKKLYALDANVCVGARNNDARSTASAFGYSSISLNQLDEKLLQFDYIFNTI
                     PDCILTKERLEKVSQEVTIIDIASSPGGVDYSATKEVGINATLCLGLPGKYSPKTSGE
                     ILVNAIENIINERRD"
     gene            27522..28112
                     /locus_tag="L3M60_000244"
     CDS             27522..28112
                     /locus_tag="L3M60_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HBN6224951.1"
                     /translation="MRLEGLTIGVGFTGSFCTYDKIFIELENLVKEGANVHTIFSDVS
                     QNIDCRFGNSEEFMKKAYELTGNKPIVTIEDAEPFGPKGIADIIIIAPCTGNTAAKLA
                     NGITDSPVLMAAKGHLRNDKPLVISISTNDALSFNFKNIGILLNSKNIYFVPFGQDNC
                     KAKPNSMIAHTELIIPTIELALENKQLQPVIKSPHQ"
     gene            complement(28319..28519)
                     /locus_tag="L3M60_000245"
     CDS             complement(28319..28519)
                     /locus_tag="L3M60_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224952.1"
                     /translation="MKKLKIISIFSLIISVILTIGGIGIVTYYVNNLFIRGLSVFVLI
                     MSSSFVSTTVRLIFEESKRYKF"
     gene            29023..31677
                     /gene="adhE"
                     /locus_tag="L3M60_000246"
                     /gene_synonym="adhC"
     CDS             29023..31677
                     /gene="adhE"
                     /locus_tag="L3M60_000246"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBN6224953.1"
                     /translation="MAHKEQVIIENKKENEVMSVGLDTVNNVESLVKKLAKIKEAQKI
                     FATYTQEQVDKIFLAASLAANKQRVPLAIMAQKETGMGIAEDKVIKNHYASEYIYNAY
                     KETKTCGVIEKDEAFGMTKIAEPIGVIAAVVPTTNPTSTAIFKALLALKTRNGIIFSP
                     HPRAKNSTIEAAKVVLEAAVLAGAPEGIIGWIDEPSLELTTTVMKEVDLTLATGGPGM
                     VKSAYSSGKPAIGVGAGNTPAIIDESADIKTAVNSILVSKTFDNGMICASEQSVIVLE
                     NIYNEVKKEFKERGAYLLDKDETEKIRNIILVNGGLNSKIVGQTACTIAKLAGFEVPV
                     DTKVLIGEVESVEIEEAFAHEKLSPVLAMYKASDFDDAVRKAEKLVEDGGFGHTSSLY
                     IDDVNQREKLDKFTSAMKTCRILINTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVS
                     ENVGVKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVALKELKDVMDKKRAFIVTD
                     TFLYNNGYTKAVTDLLDEMNIKHTTFFEVEPDPTLECAKIGAKAMREFNPDVIISIGG
                     GSAMDAGKIMWTLYEHPDVDFQDLAMRFMDIRKRVYTFPKMGEKANFVAIPTSAGTGS
                     EVTPFAVITDQDTGVKYPLADYELMPNMAIVDSDMMMNMPKSLTSASGIDALTHALEA
                     YVSMLATDYTNGLALQAIKSIFEYLPRAYDNGAKDPEAREKMANASTMAGMAFANAFL
                     GVCHSMAHKLGAFHHVPHGVANALLITEVMKFNSSDAPKKMGAFSQYKYPEALKRYAE
                     VASFLGLKGNSEEEKFQSLLSAIEDLKFKVGIPKTIKEFGVEESKFMDSIDEMVIQAF
                     DDQCTGANPRYPLMNEIKDMYLNSYYGR"
     gene            31953..34172
                     /locus_tag="L3M60_000247"
     CDS             31953..34172
                     /locus_tag="L3M60_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6224954.1"
                     /translation="MTRRSKSFTLIIILFLTSLFLTMGYIYIEDTKNLLMSEAKIHIK
                     EVAIQGSQLAQRQIEKDLDVLNIFSNYYASNPDIPNEEKMKNLLDELENQKFYTMAIV
                     DINGNAENTKGDKFSVKDREFFKNSIKGKKYVSSPYADEVNKSIKKITISVPLLNNDK
                     VVGVLYCTYNINTLMKLINVSFYENNSISYVVKNDGTVILHPQEDSISKNIYKLLKQD
                     NDIREVNSLKKELQENKTGATVLKMLEERRYLGYATMDNGNSENNYIKDWNLIFSIPE
                     TVVFSNSEKIINRAIYAVLFIILIFIAIIFYIIYIKKSNEKEILSLAYEDKVTHIGNQ
                     NKFYRECSKYLLDKPSLNYIIVYFDINNFKMINDTFGYEFGDNLLITIAKALKEELTE
                     GEVFARLSSDYFAIFCDYKNGRNKIIRKLDSIRNNIESNLSIVFEISLCVGIYFVEED
                     EVDIQKAVNKANMARSVAKGKNINYAIYNEDVRNKLSEESMILDDIKIALVKNQFEVY
                     YQPKFSLVNGEMIGSEALIRWNHPEHGFISPAVFIPIAEKSKLILKIGRFVFEKVCTD
                     LSEWKKQGKKIVPVSVNLSRVELYQPDIVKFINKTIQMYNLSSDLIEIEITETVAINE
                     LNILKNVLNELRKYGFSISMDDFGTGYSSISCLRDMPIDILKLDKSFLDGIEHDERSR
                     NIAKSIVSLAKSLDLVVIIEGVESKEQAELMKQFGCDLVQGFYFARPMPAKNFLDLL"
     gene            34426..35088
                     /locus_tag="L3M60_000248"
     CDS             34426..35088
                     /locus_tag="L3M60_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224955.1"
                     /translation="MIDVSIDKKLKERWPNAKLGCIQAKVAVNKSSEKLINEINEFCD
                     TLNKNLKVEDITKLDKIKDARNAYKELGKNPSRYRTSSEALVRRIIQGKGLYTINNIV
                     EINNLISIKSLYPVGTYNVSKLHSPVCFTVGDEGEQYKGIGKELINIENLPILSDSVG
                     KFGSPTSDSERAMITEDANEILICIFSFSEESDIEEYFGYAKELLKEYADGKDIETKI
                     IK"
     gene            35458..36867
                     /locus_tag="L3M60_000249"
     CDS             35458..36867
                     /locus_tag="L3M60_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HBN6224956.1"
                     /translation="MIDGKEVKQESGQKNLKFKIAIVVGILFVAYIGMAIFFRNHFYF
                     GTTINGVRASGKTVEQVNDLLTSSTNSYTLNLDERNNKKEQIKAKDIDLKFTPDDKVQ
                     KIKDSQSAIGWIGGIFKSKEYNNMVTLSYNKDLLEKRFNALSCLDSKNVVEPKSAYPK
                     YDSTKNAYVIVPEVLGNKVKKDDFYKLVQNSINSGNTSISLDESKVYEVPNNTKDSEK
                     LKQAIKTLDKYVGVVVTYDFSDRKETLDGSEINKWLKVDSEKNYDITFDNDAMVEYTR
                     GLSKKYSTFGDSRPFKTATGKNITISGGIYGWLIDKKKEAEALVDVVKAGKNVTREPI
                     YIQKAVSRNRDDIGNTYVEISLSNQHMWFFKNGEMLVSTDIVTGNLARGFATPSGIYP
                     LNYKARNVSLTGQGYSSPVSYWLPFNGNIGIHDATWRNSFGGGIYKSSGSHGCVNTPF
                     SKAKKIYENIEPGTPIILY"
     gene            36982..37257
                     /locus_tag="L3M60_000250"
     CDS             36982..37257
                     /locus_tag="L3M60_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224957.1"
                     /translation="MNEKLTKILEKAKNDDEFRKELIKSREQRDVMDSFCKVVTSRGY
                     DITVGELFSIGEEYTSNLLKSVNGGATYPIEDWSDWYEDFFIMLSNI"
     gene            37887..38198
                     /locus_tag="L3M60_000251"
     CDS             37887..38198
                     /locus_tag="L3M60_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224958.1"
                     /translation="MAFEIIKSIVEAEQTADSIKVKAVTDAESIRADAVNKCESIFAD
                     VKKQAKLMEETLIEKAVTDSRAEVDKILANAKSECLKIEKTAKERKSKAIEAVIGKVV
                     R"
     gene            38200..40125
                     /locus_tag="L3M60_000252"
     CDS             38200..40125
                     /locus_tag="L3M60_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HBN6224959.1"
                     /translation="MAIVNMNKISIVGLEAQKSQILKLLMNRGFVQIDDSAFLTEEEE
                     LKNYLVKDSEESEVIMLEQKMSHVNQAINIVSSLVKQKKSLLAPKREFNKIDSEKEEQ
                     VYAEALELNNLHKEIGTIRSNINLLNNNKSMLEPWVNFDIPLENMETKYTKTILGTVP
                     ISANIDGLRVKLEDEIPESVIGIVNSDKLMNYVYLITLKETFDDVLEVLKEFNFSVVS
                     LPNEEGTPLQAIKKYNEMIIRQENVSKDKTEEIKRHAESIHMLENLYDFYTIERDQKK
                     IIERLVKTKTTFCLNGWLPSKRADELIKEITEKFDCCVQTEEGSKEEGFPILLENNKL
                     VTPFESVTNMYSYPSTKDIDPNTILTFFFVVFFGMMLSDAAYGLIIALVCGFVVYKLK
                     IQKGEGNLIKLIGICGVSTTVWGLIFGGVLGDLIPIKALINPLEDVMTLMGMSLLFGI
                     IHIYVGLGIKAYTLIRSGKVIDAIFDIGFWYLCITGVCLLIIPFVAGDIGVFSEVGKY
                     LAIIGAIGLVLTQGRSFKGIPVKLFKGFSSLYGITSYFADILSYTRIMALCLTTGVIA
                     QVINLLGAIAGPILAVVIGVVGHTINLLINALGAYVHTSRLQYVEFFNKFYEGGGVPF
                     VPFKYKTKYTSINKKEM"
     gene            40128..40619
                     /locus_tag="L3M60_000253"
     CDS             40128..40619
                     /locus_tag="L3M60_000253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HBN6224960.1"
                     /translation="MEFLNALGNGQFLAISGAAIAALFAGMGSAKGTSIAGQAAAGVV
                     TEDPSKFGQLLVLQLLPGTQGLYGFIIAFLILSKAGIIGGDAIPTSAQGLQLLMAGLP
                     IGLVGLVSGIAQGKAAAAGVGIVAKRPEELGKAITFAAIVETYALLGLLVSFLAYNGI
                     AIG"
     gene            40671..41234
                     /locus_tag="L3M60_000254"
     CDS             40671..41234
                     /locus_tag="L3M60_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HBN6224961.1"
                     /translation="MGNEQKMIDRIIADAKQEAQEILDKAKSEADLKVNSANEKAEKE
                     MASYTKLAEAEAEKAASKEISGAYMEAKKQILSKKQEILEEVILEAKNKLLNLKDNEY
                     EEIILNMIEKSNCTDDSEIVLSKKDKKTLKDVLSKKGIKVSDETRDITGGFIVKKGDI
                     EYNYSFEAIIAVEHEYIEQIAAEILFN"
     gene            41249..42226
                     /locus_tag="L3M60_000255"
     CDS             41249..42226
                     /locus_tag="L3M60_000255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HBN6224962.1"
                     /translation="MAEEKTYPYAVARIRVLEKQLLNRQMFIQMAEAKSPEDSLRIIA
                     EAGYADTSNNNIHNFENILTQELSKTYEILKSLAPEEKFVDVFLYKNDYHNLKVLIKE
                     EISGVDGEKYLIDGGTIPLIKLRESLANRSFSDLPKVMSSAILEAFDVYSKTQNGQMV
                     DIVLDKATFTSMKETAKESQNKFVIDYVMKVCDLTNLKSFIRVKNMKKDFDMFMNVFV
                     SGGSLDKEKFLEAFSSDTPASCFKSTSYSDVCKNGMDSGFTVFEKLCDDYLMEYVRGA
                     KFKPLTLEPLIAYLYAKESEIKTIRIILTSKLNNIDADTIKERLRDAYV"
     gene            42213..42536
                     /locus_tag="L3M60_000256"
     CDS             42213..42536
                     /locus_tag="L3M60_000256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HBN6224963.1"
                     /translation="MLMYKVGVVGDKDSIMGFLALGIDIFPAYDSDEIKKSIHKLVED
                     EYAIIYITEQASLLAKESIAKYKDYQLPAIIVIPGIGGSMGLGMNEVRESSKRAIGAD
                     ILFKE"
     gene            42653..44431
                     /locus_tag="L3M60_000257"
     CDS             42653..44431
                     /locus_tag="L3M60_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HBN6224964.1"
                     /translation="MKTGTIVKVSGPLVVAEGMRDANMFDVVRVSDKHLIGEIIEMHG
                     DKASIQVYEETSGLGPGEEVVSLGMPMSVELGPGLISTIYDGIQRPLEKMYDISGTNI
                     TKGVEVSSLDRTIKWEFKPKKSVGDKVVAGDIIGGVQETAVVECKIMVPYGVKGTIKE
                     IYSGEFTVEDTVCVITDEKGNDIPVTMMQKWPVRKERPYREKKTPDSLLVTGQRVIDT
                     FFPITKGGVAAIPGPFGSGKTVTQHQLAKWADADIVVYIGCGERGNEMTDVLLEFPEL
                     KDPRTGESLMQRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRW
                     AEALREMSGRLEEMPGEEGYPAYLGSRLAQFYERAGKVNCLGMDEREGTLTAIGAVSP
                     PGGDTSEPVSQATLRIVKVFWGLDASLAYKRHFPAINWLTSYSLYQEKVDVWADKNVN
                     DNFSAQRAQAMKLLQVESELQEIVQLVGVDSLSDGDRLILEVTRSIREDFLHQNSFHE
                     VDTYTSLHKQYRMMGLILKFYKSAQDAIKQNVTINDIIKLSVLEKIGRAKYVEEGDID
                     AAYDSIEDEIDNELADLIKKRGAEEL"
     gene            44428..45801
                     /locus_tag="L3M60_000258"
     CDS             44428..45801
                     /locus_tag="L3M60_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HBN6224965.1"
                     /translation="MIKEYKSIQEVAGPLMLINGVEGVKFDELGEIELSNGEIRRCKV
                     LEVNGDTALVQLFESSTGINLAESKVRFLGKSLDFGVSPDILGRVFSGMGRPIDGGPE
                     IIPDKRLDINGAPINPAARDYPAEFIQTGVSAIDGLNTLVRGQKLPIFSGSGLPHANL
                     AVQIARQAKVRGTDSKFAVVFAAVGITFEDAEFFISDFKATGAIDRSVVFVNLANDPA
                     VERVSTPRMALTAAEYLAFEQDMHVLVIITDITNYAEALREVSAAKKEVPGRRGYPGY
                     LYTDLATMYERAGKMKGHEGSITMIPILTMPEDDKTHPIPDLTGYITEGQIILSRELY
                     KKDIQPPIDVLPSLSRLKDKGIGKGKTREDHADTMNQLFAAYSRGKDAKELMAILGES
                     ALSEIDLMYAKFADEFEKEYVSQGFRTDRTIEQTLEIGWKLLSMLPKSELKRIRDEYF
                     EKYMPKE"
     gene            45807..46475
                     /locus_tag="L3M60_000259"
     CDS             45807..46475
                     /locus_tag="L3M60_000259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HBN6224966.1"
                     /translation="MARLNINPTRMEMTRLKKLLKTATRGHKLLKDKLDELMKQFLEI
                     VRENKRLREEAENALDAAYKNFIIARAVMSQEYLGSALMMPKQSVSVDVSTRNIMSVD
                     VPVFDFKTENNQSDIYPYGLAFTSGELDSAMEAFSAAMQPLLRLAESEKSAQLLAQEI
                     EKTRRRVNALENVMIPNYIETIKYIAMKLEENERASTTRLMKVKDMVLKKALEEKKKN
                     DLVV"
     gene            46752..47801
                     /locus_tag="L3M60_000260"
     CDS             46752..47801
                     /locus_tag="L3M60_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6224967.1"
                     /translation="MKEFIFTCLCIVVITFMTSCISTTPQTTQGKGNNKLTIYAALEE
                     EQIAYYMEGFREEYPNIDVEIVMDSHGVIASKLLAEKDNPQADVIWGLSAMNMIELKE
                     DGALEAYKPKHYDKVDKKFKDTEDDVSWVGMSVPETAIVVNYKELKKLGIDIPKSYED
                     LIKPEYKGLITMPNPASSGTGYLTVSGLIQMMGAKDAYNYMDKLHKNIATYTHSGSKP
                     AKLAGSGEYPIGITLGYRATQQLSSGEPVEVVFPKEGSGWDLEANALVKKDNIKKEAK
                     LFLDWAISDSAMDRYSHEVAVTSIKTDNPVPKGYSKQPFEQLIKEVDLSSAAKNRSTV
                     LKTWESKYGSKSESK"
     gene            47897..48886
                     /locus_tag="L3M60_000261"
     CDS             47897..48886
                     /locus_tag="L3M60_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6224968.1"
                     /translation="MSYLKIKNVFKNYDQKRVLNNISLDIEEGEFLCLLGPSGCGKTT
                     LLRIIAGLEDVNSGDIILQDKDITNLEPSKRGFGIVFQSYALFPNMTAYNNIAFPLKE
                     RKISKEKIDNKVKEVLEIVGLTNEAHKYPKALSGGQQQRIAIARALALEPKFLLLDEP
                     MSALDAKVRHKLRMDIKRLQKELNITTIMVTHDQEEAITMADKIAILNGGDIMQIGTP
                     EEIYQNPQNLFTAQFIGDTNCFDNGDSILTVRPEYVQIEKSTKENYQGIISNIEFRGN
                     LLRVEIKDKLNENFIISDVSIKEWVNLNLVEGDLVKISIDEKYYLKYPKVKGA"
     gene            48891..50609
                     /locus_tag="L3M60_000262"
     CDS             48891..50609
                     /locus_tag="L3M60_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HBN6224969.1"
                     /translation="MNNNAIGYKSISLKSKMNLDIKKCMLTVLVMSLVIFLLAPIIYL
                     FVQAFLDKDGVFVGLQNFQEYLASETLLNSLKNTIFVSVISTVISLALALVYAYALTR
                     TNLKFKGFFKSLAMFPMFAPTMMHAIGLVYLFGNQGLVTNLLGIHVPIYGAVGIILAQ
                     IIYVFPQSFQILYISLNSTDYRLYEASNTMGISKIREFISITIPSIKYSLISSAFVSF
                     TLCFTDFGAPKVIGGEFNVLSIDVYKQIIGQQNMPMGATVSILLLIPAIISFIVTKKI
                     EKNQNSYITSKSIPYKVKTNKLRDSILGGIVGIIAFGIISVMLTVILSSLISVWPYDL
                     RLTLEHFSMKNSIDGIKPFINSIKMSLLTAILGTSFVFLFAYLNEKTEKAPYLKSFGY
                     FLSTLPMALPGLVLGIAYVLFFNKLEFDFMDTIYLKNLFNGLYGTLLIMVICNIVHFF
                     SVAFITATTAIKKVDKELDMVAKSMGINSASILKNVTIPLCLPAVLENFMYFFLNSMV
                     TISALVFIYTASSKVAAISIVQLDDKGSTAQAAALAVLILITNILVKAIHEFIKNRLE
                     KRTNFN"
     gene            51037..51768
                     /locus_tag="L3M60_000263"
     CDS             51037..51768
                     /locus_tag="L3M60_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HBN6224970.1"
                     /translation="MGRAKYYDIYIDLKNRIENKEYLYQDLLPSEYTLIEEYNCSRNT
                     IRRAIQELANQGYVQSKHGKGVFIIYKANKQSEFLLGGTESFKESAIRNKKEYKTKVV
                     HFEEMVIDDKKSQITNFEVGTEVYYIQRVRILENKPSIIDHNYFMKDIVKDINEEIAS
                     NSIYEYMESELNETIVTTKRTMTVEKATKLDKKYLELGDYNCVAVVSNNTYNAQGIMF
                     EYTQSRHIPDGFIFFHQSQRINNIK"
     gene            complement(51784..53541)
                     /locus_tag="L3M60_000264"
     CDS             complement(51784..53541)
                     /locus_tag="L3M60_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6224971.1"
                     /translation="MKGLYQMNIKEIPFSYFGSYMSISYSDELGFTLKSLRGKSKKNM
                     DVLRIIPTVNNMPVDYEFEANYFSILIKFNNGSISICFEGDSKIVFYGKGKNVGLKLD
                     TLPIYNFEYNYLMGKKGSEYCVINSYKNLTKFLVFSVNGKVGLSQNIFIDDCASTNKA
                     NNTSIIDVKSNSDNEFICVIEDIPTHMKVPDYKEYNFETSLNSTKKLFDEYYLAQPKV
                     DEKYKNSLLKASYINWSSFVKPQGYLKRYTILASNSTFLGAWSWDHCFNALALAGVND
                     KLAFDQMAVLFDYQDEYGQIPGSLSDSTIRWNFTKPPIQGLFFSKMMKKINFTKETLE
                     KIYNYIEKQVLFYVKFKDSNMDGIFEYHHGNDSGQDNSTIFKGAMVVDTPDLTAFIIN
                     AMDMLSKVAEKLNRNSEKEYWSNKADELTKLFLNYFIVDDLPVARETVTGEILSNNAL
                     LPLVSLVLGNRLPKYIRDKMIDLVTSERYLTKWGIATEAIDSDEYQDDAYWRGPIWAP
                     TTLLFVEALEECGENELAIDIAKRYCELVDKNGFAENFNAITGEGLRDKSFTWTASTF
                     IYLAAKVYEYNSQRINNIK"
     gene            53771..55204
                     /gene="treB"
                     /locus_tag="L3M60_000265"
     CDS             53771..55204
                     /gene="treB"
                     /locus_tag="L3M60_000265"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBN6224972.1"
                     /translation="MRKNKILNSKESISKIIEYVGGKENIQSATHCVTRLRLILKDTS
                     KANISKLEDIDIVKGCFNAGGQFQIVIGTGVDKVYKDLIDQTGAEEVSKEEIKQQVNE
                     KSNKFQKFIRILADIFIPILPAIVASGLLMGVNNMIANPGIFYEKSVIEVNTDIAGIS
                     GIINLIANTSFTFLPALIGWSAAKKFGGNPLLGVVLGLVLVHPALMSAYDFAANPSAA
                     PTWDIFGMKVAQVGYQGQVIPVLIATYILAKLELFLKKTVPDAVQLVLVSPIALLITG
                     MLTFVVIGPITMTGANMITNGVVYLFEVAPVFAGALYSFISPPLVITGMHHLFLGVNL
                     QMAGTLGYVTLWPISETVTLAQGAATLAMFFILKNNKNLRGVSITSTISAWLGITEPA
                     IYGINLRFRYPFIAVMVASAIGGAFISYHNVKATAVGVGGVFSFLSVMPQYWGVYFTG
                     MAITFVITIGLTYLFYKSGKFKTNDNI"
     gene            55267..56925
                     /gene="treC"
                     /locus_tag="L3M60_000266"
     CDS             55267..56925
                     /gene="treC"
                     /locus_tag="L3M60_000266"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBN6224973.1"
                     /translation="MLDFKKSIVYQIYLKSFNDTTNNGLGDLNGITEKLDYLEFLGVD
                     YVWITPFFVSPQNDNGYDVADYYNIDPIYGTMDDLENLIKEAKKRNIGIMLDMVFNHT
                     SIENEWFKKALKGDEYYKDFYIFKNPKEDKSAPTNWESKFGGNAWEFVEEFNQYYLHL
                     FDKTQADLNWENPNVRKELKNILKFWIDKGIKGFRFDVVNLISKPEKYEDDLQDDGRR
                     FYTDGKKVHQYLKEMCEESGILENDMLTVGEMSSTTIENCVKYSNPDEKELSMCFNFH
                     HLKVDYKNQNKWMLQDCDFIKLKKILNEWQIGMQKGNGWNALFWCNHDQPRIISRFGD
                     DKKYYKESAKMLATMIYLLRGTPYIYQGEEIGMTNACFTNIKQYVDVESLNYFDILKS
                     KGMKDGEIYNILQERSRDNSRTPMQWNNSKNAGFSESKSWIEVINNYRNINVENSLKD
                     KDSIFYYYKKLIALRKKYDVVAYGDFRPLLEEHDSIFAYKRCYEKESLVVLNNFYDKE
                     IDVVLENEELEKYKCIISNYGDRKIVSTITLRPYECIALFKNEK"
     gene            57178..57882
                     /locus_tag="L3M60_000267"
     CDS             57178..57882
                     /locus_tag="L3M60_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6224974.1"
                     /translation="MKEPIYKVIENHVRELINSDSLKEGDLIPSEKQLSEEFNVTRMT
                     VRSALNNLVKEGYITRQRGVGSIVLANNIYDNISSVSGFTKEMESKGYKVSNILVSLE
                     IVQADEELSGKLNISLEENVWEIKRVRLANDARVSYMITYMPVKLFPNLNKTHCENSL
                     YNFVEEVCAYKIAMSEREVQAVISNKECMDNLKLEEPEPLLYISQICKLQNSEIFEYS
                     HTYHYGYTLTLNAVVE"
     gene            58082..58393
                     /locus_tag="L3M60_000268"
     CDS             58082..58393
                     /locus_tag="L3M60_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6224975.1"
                     /translation="MIKIMLACSAGMSTSLLVTKMEDAAKENGIEAKIWAISEVNLKN
                     EIENCDVLLLGPQVRYVLGKATEMAKPYNIPVEVINMMDYGRCNGKAVLDRAVELNSS
                     K"
     gene            58415..59728
                     /locus_tag="L3M60_000269"
     CDS             58415..59728
                     /locus_tag="L3M60_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6224976.1"
                     /translation="MEKFMSFMDKYIVPVAAKIGAQRHLVAVRDAFIVMIPITMVGAL
                     GTLINNLPLEAYKNLMASIFGENWTTFGGDLWWGAIGTMAVFLVIGVAYFLAKSYESD
                     GLQAGLIALSIFFIMAPQIGKIVPEGGTTVVEGWGMIQQTYLGTAALFSSILIGLLST
                     EIFVRLSKVKKLTIKMPDGVPPAVSRSFAKLIPGMLTIMIFTVIGIFIKMLSNGSFLT
                     DILNTYLGAPLSNVADSLGSTMLIAFIIHILWTVGLHGANIALPFTETILMKLGGENA
                     ALAQAGATEGYHVLAGAFFDAFVYLGGSGMVLGLIVALLIAGRRRKEMIVLGGPPAIF
                     NIGEPLIFGLPIVLNPIFMIPFVLAPVICSAVSYLAIDFGLVAPVILPKIPWVTPPIL
                     GGAMATGDWTGGALALFNLILSILIYIPFVIASEKMEANKLKINN"
     gene            59805..61115
                     /locus_tag="L3M60_000270"
     CDS             59805..61115
                     /locus_tag="L3M60_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBN6224977.1"
                     /translation="MKKGLKIVTIGGGSSYTPELVEGFINRYEELPVKELWLVDIEDG
                     KEKLEIVGNLAKRMVKKAGIDMQINLTLDRREALKDADFVTTQLRVGLLDARIKDETI
                     PLSHGVMGQETNGAGGLFKALRTIPVIFDIIKDVEELCPNAWMVNFTNPTGIITEAVF
                     KYTNFRRYIGLCNVPIHLKNDVAKLFNVEGDRISMDFAGLNHMVYGLNVSLDGEDVTK
                     EAIDKFVKADISMQNIKAIDFNAEFVKSLGAIPCPYHRYYYKTKEMLEDELREFKEGK
                     ARGQVVKELEEQLFELYKDEKLDVKPPQLEKRGGAYYSDAACNLISSIYNDKKDIQVV
                     NTLNNGSIRDFKDEQAVEVSSVITKNGPKPISIGYLPDSVHGLVSQIKSFEVLAAKAA
                     VYGDYESALLALCINPLIPSDDLAKTILDEMLEAHKDYLPKFNR"
     gene            61141..61872
                     /gene="chbG"
                     /locus_tag="L3M60_000271"
     CDS             61141..61872
                     /gene="chbG"
                     /locus_tag="L3M60_000271"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBN6224978.1"
                     /translation="MTKIIINADDFGYCEAVNYGIVSVHNNGIVRSTSMMANMPGVEH
                     GVGLLKENKSLNCGVHMTLSCGRPLLSNLKTIVDKDGFFIRRITDEIIEKMDYDEIYR
                     ELCAQIDRVKGLGIDISHLDSHHHIHTLISLKPVIEKIVNKYNLPIRGGFEYNLEYSK
                     IVPLIDSFYKENVCEEYFIKNIDEIMKYDVVDIMSHPAFLDDYILNSTSYAIDRTKEH
                     KILTSKKVKEFLDKNDLVISSYRDI"
     gene            61885..62202
                     /locus_tag="L3M60_000272"
     CDS             61885..62202
                     /locus_tag="L3M60_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBN6224979.1"
                     /translation="MDEKMIEISFGIIGFAGDAKGLAFEAIKEAKSGNIDKARECIEK
                     SKESIHKAHKFQTELIQNEANGNKTEISVILIHAQDHLMTAMNFQQLAEEFIDVYERL
                     ESK"
BASE COUNT        24420 a         6579 c        10921 g        20345 t
ORIGIN      
        1 catagcattt ttaaaacttt ctacttttat tagttcgaat aactgttact attaaatatt
       61 aatactaatt atatacatat cattatataa tatttataga ttattatata agtagattat
      121 tagtaaaatg tatgcattat taattaaaat aaattacata tgttatttaa tattttattt
      181 aaaatattaa aaaatataga taaaaattgt tttattaaga aaaaattaat atattgacaa
      241 aatttaagtg ctcatttaaa ctattaattg ataataatat tcaatattaa ataataggag
      301 gagttatgaa taaatatctt ctaataaatc cagtagcagg aagaatgtat ggagaaaagt
      361 ttcaccttgt caaagaaaat ttgatgaaaa aagggtacat aatagcagaa tgtgaaccac
      421 aattagaata tgtaaaaaag cagtacaaag agtatacaaa aaagactgag aatacaatgt
      481 tggattgtag atgtccagaa agtataaatc ttttaaaaag aaataatttg ataaatagtt
      541 ttgaagtacc tatgatagaa ccaatattag taaggacttg tagggtttta tatgataaat
      601 atataaaaag taaagatgat atgctgatag taacctgtcc atgtacacaa ttaagagact
      661 ttgcaagtga aaaatttaag gataagagta atgctatatt ttatacatgg aaagagtttg
      721 ctgagcagga aggtgtaaaa tcgcttggaa aaatagatga atcaccaatt ccattaggat
      781 atttcaaaga tacttttgaa aaagtgcttg aagtatcaag tgaagatgaa atcttaagtg
      841 aaatacaaaa aataagaaat ggctctgaag ataagtatga catagtagaa atgttttatt
      901 gtaaagatgg ttgtaataat ggagatggtt tgtaaatggt caataaattt aataataaat
      961 ttttaaggct agtaaagagt gttctgatat tttttataat tcttttatta tggaaaatta
     1021 caaattatct tggtatttgg agtgattaca tactgccatc acctgaaaag gtatatagta
     1081 catttttaaa tatgataagt gatggttcga tatttattaa tgtatatgca agtatgaaaa
     1141 gagttttgat aggttttgca ataagtactg ctataggtat tcctttagga atattctttg
     1201 gaatttatag tggagtatac gaatatttta aatctctaat aaatttttta aggaatacac
     1261 ctccccttgc tttaatacca atgcttatct tatggtttgg tataggagag gaatcaaaaa
     1321 ttataattat agttttagca tcctttttcc caatctttac aagcacattg aagggtatta
     1381 aaaattgtga ctcaaaactg attgaagtgg gtagggtatt tgagttttca aagcttcaga
     1441 taatatttaa aataataata ccaaatgcca ttcttgatat tgcagttggg ctaaagttag
     1501 ctcttggata tagttttaga gcaattattg gggcagaact tgtagcagcc tcatctggtc
     1561 ttggatactt gatatctgat ggaaaagaaa tgtctagaac agatgttgtt attgtaggta
     1621 taatcgtaat tggactttta ggaataatta cagattacat attttcgatt attgtgaaga
     1681 aggtaagtaa gggaaagatg gtggatgcat atgaataatt tagtatgtga ttctgggtat
     1741 ataattagaa atttatctaa aagttatgaa gttgacaatg aagaacatgt agttcttagt
     1801 gatatctctt taaacataga tagaaaccat ataacagtaa ttttaggtga aagtggttgt
     1861 ggaaagacta ccttgcttag agcaattgca tcacttgaaa atataaatag tggtgaaatt
     1921 aggtttatta atgataataa agagtataga cctaatgttg gttttgtctt tcaggagagt
     1981 cgattgatgc cttggctaaa cgtaagtgaa aatattactt ttcataatca aagaaaaaac
     2041 actattttaa acaagatgct tagaaaattt aaacacaata aaagagttat tggtaaaatt
     2101 ataaatgata attttagaag tttagaaaca aatgagaatt tgaacaaatc aaataaatat
     2161 aataggaaaa gtaatattga tgtagaaaaa tatcttaaga tgatgaactt gcagaaattt
     2221 aaaaactctt atccaaatga attgtcagga ggtatggcac agagagtaag tatagctaga
     2281 gctctttctt tcaatccaga tatgttgttt atggacgagc ctttttcggc attagattat
     2341 tttacaagga ttgatatgca aaatgaagtt ataagaatac ataagtcaac taataaaggc
     2401 gtaatatttg taactcatga tattgatgaa gcattaaaga taggcaagaa aatagttgtt
     2461 tttacagatg aaagaaaaat aaaagaattt aatatagaag atgattataa tagagattta
     2521 acttcagagt actatattaa attaaaaaaa gatattttaa tgacaatgaa atcaaataaa
     2581 ggcatttaaa gaaattaata atattttaag gagtagagta aatgaaatta aaaaagctat
     2641 tgataggtat aacaatatta acgcttgcaa cagcatcttt agctggatgt gctaaaaaaa
     2701 ataatggaga aaaactttca gaaataaatt taacttatgt aaaatcacca ttaaatgttc
     2761 cttctataat tcaaaaacag gatgatttat ttggaaagga atttaaaaag gataatataa
     2821 aagttaactt ccacgaaatt actacaggtc cagagcaaac acaagcttta gctgctggag
     2881 aaatagactt tttacatgca ctaggtggta cgtcagcttt aatagctgca tctaatggag
     2941 tagagttaaa gatattaaac acttatagca ggtcaccaaa aggatttatg attttgacta
     3001 ataataattc aataaaatca gcagcagatt tagtaggtaa gaaggtggca gggccaaaag
     3061 ggactatatt acatcaggta ttgatttcag cacttgataa agaaggatta agtatggatg
     3121 atgtggaatt tgttaatatg ggtattccag aggcatcagc agctctttct gatgggagta
     3181 tagatgctgc attgattgct ggtccagcag ctcttaaagc tatgaagtca gggagtaaac
     3241 ttgtagcaaa tggtgaaggt ttagtagatg gcataatagt aactgctgtt agcacagact
     3301 ttgcagagaa acatccagaa atagttgaaa gatttatgaa ggttgaaaag gaaactttag
     3361 aatatgtaaa taataatttt gatgaagcta tggaaaaagt agctaaagaa gttgatttaa
     3421 gtcttgaaga aactaaagaa ttatatgttt ggtatgattt tagtttagat ataacagata
     3481 aagacatatc ttcacttgaa gatacacaag acttcttaat aaaaaataag ttacaagaga
     3541 aaaaagtgaa cataaaagaa ttaatatata atgctaagta gaaactatgc taaaataata
     3601 ctaagtaaga aatataataa aagtttaagt taccttatta aggtaacttt ttctattttg
     3661 aattagtaat ttaaatttat tttagaatga ttaattgata atagtgatta attaatcata
     3721 aaaagatacc tttttataga gttttgttaa gatgtctaat aatatagttg tatctatgaa
     3781 gtcagtaatt gtgaagttag taatatgatt aaatttatat attgtaaaaa tatcataata
     3841 tattaaaatt gagctgttat atgatgagta aaaaggtata ttaggaggaa ttatatgagt
     3901 tataaagtat atattgtgga agatgatata tcaataagta cattacttaa agattatatt
     3961 gagaaatatg gatttgaagt gattgttgaa gaaaactttg atgatataat gattacattt
     4021 gaaagcttta agccacactt agttctgtta gatgtaaact tacctaaatt tgatggtttc
     4081 tactggtgta ggtgtattag gcaacagtcg aatttgccta taatttttat atcagctaga
     4141 gatggtaatg tagaccaaat tagagcttta gaaagtggag cagatgatta tataacgaag
     4201 cctttttact atgatgtagt aatggcaaag ataaagagta atataagaag ggtatatggt
     4261 gaatattcaa ctaaaataga tgaaagaata gtggagttag atggattaaa gttttatcca
     4321 gaaaggttgg aactagaatt acatagtaag aatctaatta taaccaaaaa agaaggtata
     4381 ttgattgaat gtcttatcaa aagataccca aaggtagtaa gtagagatta tcttttagaa
     4441 aagatatggg atgatattga gtttgtagaa gaaaatactt taaatgtaaa tgttagtagg
     4501 ataaggaaaa gatttgagga attaggcata gataatgctg ttcagactat acgaggatta
     4561 ggatataaat taaataaaaa ttggtaagga gaaagtatga agttattttt aaaagattat
     4621 aaaggttata tttttatata ttatataggc atagttctga caattattta ctgtaatctt
     4681 atgaagttta ttagttttga tgagagttta tatattcttt tatttagttc ttttatattg
     4741 ttatgttttt taatatataa gtactataaa aatagagaag tatataatct gtttgaaaaa
     4801 ggaatacatt ctttggagga gtcagttttg tatttaggaa gctctttctt ggggagaaat
     4861 atatcagtta ttttaaaaca acaacataaa ttgtatcaat caatgataca agagcataaa
     4921 aagatacatg gtgagcattt aactttcatt aatcaatgga ttcatcagat gaaaacgcca
     4981 atatcagtta taagccttca gcttcaagat tatgagggag aagaagtaac tgaaaatata
     5041 agagaagaaa tagatagatt aaataaaggg ttagatatgg ccatgcattt tgcaagggca
     5101 gataattttc agaaagactt tatagttgaa aaagccaaat taaatgaaat tgtatctagt
     5161 attgtaaatg aagaaaaaag gttatttata aaaagtggaa tgattcctaa gattcaaata
     5221 gacaagttta cttatgttta tacagatgta aaatggatga aatttataat aggtcaatta
     5281 gttacaaatg gggtgaaata ttctaaagat tgtagcaact acataactat aaaatctgaa
     5341 gaaaataata agtatgtaat tttaagtata atagatgaag gaattggaat tgtttctaaa
     5401 gatttaaaaa gggtatttga cccatttttt acaggagaaa atggtcgtaa gtttggagaa
     5461 tctacaggta tgggtcttta tctagtaaaa aaggtatgtg atgatttagg tcataaagta
     5521 agtataaaat cagaagtagg aaaaggtaca gaagtttcaa tctcctttaa taaagatgta
     5581 aatagtttga taaagatgta aattatctgt tacaataggg tttttagtta attgtacttt
     5641 tttattaaat gaaatattca tagttaaata atccaaattt aaaagaattt atttgcaaac
     5701 cttacataga tgtaaggttt gtgtaatgtt tgcagatagt taaattttct aatctaaatt
     5761 acaattaata taagaaataa cataagggaa ataacataaa aaaagaaatt ttgtaaaaaa
     5821 ggaaggatat ttattgatga gtttagatgt tatttttata ttgaaatcag tcataatagc
     5881 tatagtagag gggcttacag aatttattcc agtttcatct acaggtcata tgattttggt
     5941 tggtaattta atagatttta aggggcaatt tgctgaaatg tttgaggttg taatacaact
     6001 tggagccata ttagcagtag tagtactcta ctggaaaaag ataaaagata gtgttagaga
     6061 atttttaaaa tttatattta ctggcggaaa agaaggaaag attggtttta gatttggaat
     6121 gaatgtaatt ataggttgta taccatttgc gattatagga gttctatttt acgataatat
     6181 aaaatcattg tttaatttac aatctgtaat tataggattt atagtgggtg ggattttatt
     6241 attagtagta gaaactttgt ttagaaaaaa gaatcattcc acagataata tagataaaat
     6301 tactccaata caagctttaa aggttgggac attacaagtt ttgtctgcat ggcctggtat
     6361 gtcaagaagt gcatctacta ttatgggtgg atggatagca gggcttaatt ctcctacagc
     6421 agcagaattt tctttctttt tagctgtacc agctatggtg gcatcttcag gaaaagattt
     6481 atttgaattt gattattcag taatgacacc aacattgtgg attgctttgg tagttggatt
     6541 tatagtagct tttattgtat caataatagt tatggaaaaa ttcgtgaatt ttttaaagaa
     6601 aaaaccaatg agagtgtttg ctatatacag aataatcatg ggtgcagtac ttgcagtatt
     6661 agctttgact aacataatta gtatatagaa aaaatagatt tattatctat agaaattaaa
     6721 ctaagagaaa ttataattaa caattatata tatgttattt caaaaattaa attaggaagg
     6781 tatgttattt caaaaattaa attaggaagg atagttaaat aatgaaaatt ttaactgtaa
     6841 ataatttaag taaagtatat ggtaaaaaaa taattttcaa tgcattaaat gacataaatt
     6901 ttagtataga agatggagaa tttgtaggaa ttatggggcc atctggcagt ggaaaaacta
     6961 ctcttttaaa tatgatttct actattgaca agccaactac aggaactatg gagctaaagg
     7021 acaagaatct tttattgtta aaaggtgaag aacttgcatt atttagaaga agagaattag
     7081 gttttgtatt tcaggatttt aatttattgg atacattaac tataggagag aatatagtat
     7141 tgccattgac attagataag gtcagtgtaa aagagcaaga taggagattg aatgaagtat
     7201 ctgaaatttt aggaataaaa gatttattgg gaaaaagaac ttttgaagtt tcaggtggtc
     7261 aagcccagag aactgccatt gctagagcat taattaatag accatctata cttttagcag
     7321 atgaaccaac aggtaattta gattcaaagt cgtcaaagac tgtaatggag ctatttcaaa
     7381 agataaataa agaaaataaa gttactacta tgatggtaac tcatgaccca ttagcagcaa
     7441 gttattgtag tagaatttta ttcataaaag atggagctat atacaatgaa atatataagg
     7501 ggagtagtag agagcagttt tatcaagata taatggatgt acttacatta ttgggaggag
     7561 ataattaatg aacataaaac agtttgctat taataatatt tcaagaaata tcaaagcgta
     7621 tttaggatat ttagcaagta ttactatatc atcttctcta ctattttctt ttgttatgtt
     7681 tacgagtcat ccagatttag atttatctca atttcctgat tatttaaaag aaggtctcaa
     7741 gatgagtaag ataattgcat atttattttt attcttcttt gtattttatt cagttagtgt
     7801 atttcttaaa agtaggtata aagaatttgg tatactttat attacaggta tatcaaaccg
     7861 acaagtaatg aaattaatct ttatagaaaa tatgataata aatatagtat cttctgtaat
     7921 tggaataata ataggcttaa tctttgcaaa gatattttta gtggtgatgt ctaccttttt
     7981 agaattatca ccattaaaat tctatatacc atttaatgcg atattgtcta ctttaatcta
     8041 ttttatgatt ttagctgtat taacatcact atttacttct tttatagtta aagaaaatca
     8101 ggtgttgaag ttacttaaag gtacaaaaac tccaaaacca gagcctaaaa catcactaat
     8161 gcttgctata ttaagtattt gtttgtttat aatagcgtat tataatgctg ttacatcaac
     8221 tgaccaatat acaacatacc tgcgcttagt tccagttaca tctcttacta tagcagctac
     8281 ctacttattt ttttctcaat ttagtgtgtt ttttataaag aaactaaaat taaacaagag
     8341 attttataga aaaaatacaa atatgctttg gatttcaaat ttaatttata aggtaaagga
     8401 taatgctaga atatttttct tgataactat aacctctgca gtagcattta ctgctatagg
     8461 tacagtttat tcattttgga aagatgtaaa gcgtcaaatt aatttaatat atcctaatac
     8521 aatatattat tcaacaatga ccttgcataa tgaccctaaa aagcctgata gcaaagaaaa
     8581 agacaaggaa agaatgagat ttatagaaaa taatctaaaa aaagaggata tagactatac
     8641 aagaattaat ggcgagttta aaactgtatt tcccaagaaa agtgatttta atgtcagaat
     8701 tatgaaagaa tctaagtact tagaaataac agaagttata ggggttaagc ctataagttt
     8761 tgaagataat gaatctatat cattattatc atcaagttta ccaggaaata agaaaataaa
     8821 agaaaatgtt actgtaggaa atagaagctt aaaggtggca aaacaagtag aagaatgtgt
     8881 tatgccagca tattataaaa atatgtatgt ggtaaaggac aatttttatg atagtataga
     8941 atcaaaattt atagtggata gatttactgc atttgaagta gaggattcaa gtaaaactat
     9001 agagatatgc agagaatttg aaaataaatt tgataatgag tcgggaatgc aaccatattt
     9061 gtttttctct aaagaattaa tgatagaaac tggtaaatta atgtattcta catttatatt
     9121 tttagcaata tttataggtt taattttctt cgttactaca agtagcttct tatacaacaa
     9181 gttttatatg gattgccaag ttgacaagaa aaagtatgaa cagttaaata aattagggat
     9241 gacatataat gagattaaaa aagcttcaac tattgagatt ggcatagtat ttttgttgcc
     9301 ttatgtagtg gctgttatac attcgatatt tgcacttact gctctgaaaa attcttttga
     9361 tttagaagta gcatcatcag ctgttttggt aatgggaagc ttgttcttgg ttcaaatagt
     9421 ttatttttta attgtaagaa ataattattt gaaagagata aaattaaatt tagttaatta
     9481 ttagttacta ttattaaatg ataacaaaaa gggtttaaaa ttaattaata ttactggaat
     9541 tatagatgtt tattttttca gtaatatttt ttattgtatt aactattaaa attttgaaca
     9601 aatataaagt ataatatgat aaatgtcaaa attataaaaa agttctactt atataaaata
     9661 taaagaggtt atatgtacat atgtgtagaa ataaataata aaaatatcga ttaatgttat
     9721 tagggggtgt tactcattag taaattagct aatacactta gaatgatgat attaattcaa
     9781 agtagaggca aaatgaaatg taaagagttg gcagaagagt tagaagtaag tgaaaggcaa
     9841 ataaaaagct ataaaatgta cttagagcaa gctggtatat ttataaattc aacgcctggg
     9901 atttatggtg gttatgaaat agataaatgt aacagcatct cattaattaa attattagat
     9961 agtgaagttt caattctaga tatgataaat tcacagttag agtacaataa tgatatttac
    10021 aaaactgaat ttaatagtat tgtagaaaaa ataaaagcag tattaaatac aagagtaaaa
    10081 agcgatacat atatggatta tttcacaata caaccacaac gtaattgtga ttataagttt
    10141 gagaaaaata agtgtaatga aataattaga gcatatacaa caaagcgtaa actttggata
    10201 gagtattatt cattgaactc aggcaatagt gagagaattg ttcatccata tggtttgttt
    10261 aattataagt cagatactta tatggtggct ttttgtgaaa aaagatttaa gtttatagac
    10321 tttaaattat gtagaattaa aaattataag gttttagaag aaaagtataa tgtagacaaa
    10381 agttttagtt gggatgaata tagtaaaaat agtataggaa tttataaggg agaagaaatt
    10441 aatgttgtta taaaaattag tcatccattt tcaacaatta taaaggagaa agtttgggtt
    10501 aataatcaac aaatcataga atatgatgat aaatctataa tgtttaaagc taaaatgaga
    10561 ggttatgagg agataaaaag ttggatttta agtatgggag cttatgttga ggttgtagat
    10621 ccagatagcc ttagaaacga tatattatca gagattgaaa aaatgaaaaa aatttactga
    10681 ttttttcaaa taatttatac ttggaccaat atacttcacg gtctagtgtt aatatattaa
    10741 ttaagagagg tgatattatg aaaatgaaaa ttgaatttag tacaagttgt ataccaatat
    10801 cttataattc gctctttatg agtattataa aagaagcaat aaaaaaatct aatgaagatt
    10861 actataaaaa catgtactat tataaagaaa agaataataa aaaaactaaa aattttacat
    10921 tttcggttta tgtaaaaaag tatagtattg aaggcgacaa ttttattata gaagataaag
    10981 taatattaaa tataagtact ccagatttag aattgggttt tcatatatat aatgggttaa
    11041 tgacttctaa aaaatgctta tataaagact atgaacttac aagaattaga atcgatttat
    11101 caagagaaaa gaaggtaaca gaagaaaggg tactatttaa tgcattgtct ccaatctgtg
    11161 taaagtctaa agaaggaaaa ttcttggaga taactgatga tagatacata gaagaactaa
    11221 actatataac taatgaagtg gttaaaaatt ataggggtag tggattaaaa agaaagttag
    11281 aatttgaaaa tatagatctc aaaaaggtag tagttaaaga gagtttaaga gaatttaaaa
    11341 aaataacagg taaagagtat caatatatta atggttataa aggtaaattt atattgaaag
    11401 gtgatattga tgatttaaat attatctaca atctaggact tggctttagg cgtgctcagg
    11461 gatttggaga tgtagatatt ttagaatgga ggtgagataa atgaagttaa gagtttatag
    11521 aggagattgg ttttttaata tgggtatagt tggattttta aatataataa agaaatcaaa
    11581 taaacaagca gaaatattta ttatggaaga ttatatcgaa tttgatagtt catttcttga
    11641 aaattttcat gaatattatt ttaattactt tatggatgaa tatgatgtat caaaaaggat
    11701 taaaaaaagt attgattaca gtataaattt tattaaatcc aaacctgata aagtcaaaga
    11761 tggaattaaa aaaattaaag acaccgtaaa acaacaaaat gataaggtta aaaaatttga
    11821 tgaagaaaaa tttaacttga taaaagagaa gttagatagc atttctaaga taaaaagtta
    11881 tgatgaattt gatagtttag aaattttagt ggatgagact atagatatat ttaaaataaa
    11941 atcaataaat gacaggctta cagcaaatct ttataaatat gtagttcaag ataattattt
    12001 tggacaagtt agttttttta atgttgctaa ggctaagtta gatttggatg gattaaaaca
    12061 ggtaatgttt aatgattatt taagacaaat tatatatttt ggagagttag aagatttatt
    12121 agaagagaat gactatgaca agttgaaaaa ctatttgaat gatagattaa atagtatagc
    12181 taaggatata agtgaaaaaa aagttagtaa gtctagtgta aatactatag aaaaaattat
    12241 gaaggaaata aataaaaact ttataaagaa aaataaaagc atagaaaata taaaagagta
    12301 tatggatagt ttagaaatat gtgaaatgtg tggtttatat aaaggaatat tagatgaata
    12361 ctcagaaagt aattttgctc cacttggagt aagtaccaat aatgctagga atatgttttg
    12421 gaagcaggag tatgtatcat caatatgtga tgtttgtaag ttaatattgt tctgtactcc
    12481 agcaggggct acttatacaa gaaaggatta tttgactaat gaagaaaatg agttttattt
    12541 atttgttaac atggatactt ctataaatga gctttttgat agaaacaata gcttgaaagc
    12601 tcaaaaaagt gagtcttcaa attctaaaga tgaaaatcct tttaatcaac ttataaaaag
    12661 tatagtagaa gaaaataaat taaaaagtga atggcaactt cgtaatatac tttttgttga
    12721 atttaaagca agtatagatt ctaaaaaatg taaaataaat tattttaata ttccaactta
    12781 tttagcaaag ttttttgtaa aagaaaataa aaaaatacaa agtatatatg attataaact
    12841 taaatcaaat gtattagatt taattttaaa aagtaaagac ttaaaacatt taattaataa
    12901 cactttaagg aatatagtta agagtaattt agaaagtaat aataaatcaa acatatcagg
    12961 gttatcttgt ttcaatgttg ttcaattaag aacaatatta aataactata agagaggagt
    13021 gtataaaatg gactataaaa agttgagaaa gatttatgag tcaggttgtg aaatacatga
    13081 ttattatatt gataagaatg ctaaaaataa aatagaagga ataacatata aaattttaaa
    13141 tttaattaag gttggtaata aaaatgattt tatgcaaaca attttaagga ttttttcacc
    13201 aagtgaaaaa acaccaccag aagaaatttt acagatattt gttgaagaag atttagattt
    13261 tgaatctgta gggtatgcat ttgtaacagg attaatttct ggaaaatatg aaggaaaaga
    13321 taaactatca aacgagaagg aggaaaaata agatgaaaag aggattaaca tttacagtaa
    13381 tagcacaagc tcaaagttta aactatggtg aagggatagg taatatatca gaattaaaaa
    13441 aattaagtag agctgatgga aatatatata catttgcatc tcgccagtgt ttaagatatg
    13501 acatagtacg attagcagca gaattgttta attggaattt acaggttgtc gataaagaaa
    13561 aaggtactat tcaatttaaa gataatataa gtataagaga atcagaagaa atggatttat
    13621 ttggatatat gaaaacaaat aaaaaagatg agaaagataa aaaaggaggt tctttgacta
    13681 gagaagctgc tgtaagatta agtaatgcga tttcccttga gccatataga agtgacatgg
    13741 actttttaaa taataaagga ttagcagata gaataggaga acatcctaat cttgctaaca
    13801 tagagcaaca tttaagttat tatacatata cagtgactat agatttatct aaaataggga
    13861 aggatggaga gatagaactt gataataaag agaaatgtag aagagtagta gaatttttag
    13921 aaataataaa agtattgaat agaaatatta gaggtagaca agaaaattta tctcctctat
    13981 ttgtagtagg tggagtatat gaaatagcaa atccattttt cttgggtaga attaaattaa
    14041 aaggagataa aaatggattt aaaataaata aacaagctat agaagaagta gtccaaggta
    14101 cttttttagg aaaatattta aaagaattta cctatgttgg aatggtagat ggggtattta
    14161 taaataaaga agaatttaag ggattgtttg aagataattt tttaagtgta gataaatttt
    14221 ttggacaact agttaaagaa gtaaaggaat attatggggt gaattaatat gaaggttttg
    14281 aaattagatt tatttcaaga aactgcatgc tataaaaaac catttgctat gaagattaca
    14341 gagacatatc cactgcctcc atattctaca ataaatggat taattcacaa aatattaaat
    14401 gctacagaat atataccatt taatataagt gtacagggaa catatgaaag tatatttaat
    14461 aattatcaga cgacatattt ttataaaaaa gatagtatta catctatgcc aatgaatagt
    14521 catatgctat tgaatgtaaa tttgataatt catataggtg gggattttga tttattgaaa
    14581 gaaatatatg atagtttatt aaattataac gagcatttat cattaggtag aaaagaagat
    14641 ttagtaagaa ttactgatat tagatttgta gaagttaata aatttgagat agatgaggac
    14701 atacaattag atgattatga aaattataag ttatcagaat gtgatataag aatgcctata
    14761 tatataccaa aaacaagttt acaagataaa gatatagatg gtataagtta caggctcaat
    14821 aattactatg ataataatgc tgtgggagat aaaaaaagag tttggaataa ggtggatagt
    14881 tattatgttg aggatggaaa tactatatca gttggagata tattattaga taatgaagga
    14941 gatttattat attttaataa tataaattag gagggagagt atgttatatg ctaaatctaa
    15001 tccagtggaa actttgagag aacatactga tgaactttta aaacaaatga atgtgttaag
    15061 agaaagttat ggaacaaata taaatagctt aaattttcta gaggaggaga tattttggca
    15121 attacttgac tttgttatag aatttcatga tattggaaaa gcattttcac catttcaaga
    15181 gttaataata agtagaatgg atactaaaat aaatgaacct aaaatagtaa ctcatctaga
    15241 aaataatgta ggtcataatt atctttctcc tgcatttatt gattatagtt atatagacaa
    15301 gaaaaaaaat aaagaactta gagcagtatt aaatcaagtt attgtatatc atcatgaaag
    15361 agatatcttt atagataaag attttaaaaa tttaatacaa aaaatattag atgaagattt
    15421 aataaataaa gtttatgaac tacagcatga atttaaagtt agatatccaa taaaaacaga
    15481 aaaattgagt aaagtatatt tacaaagtgt agaaaaaaga atagataaaa atcacaagta
    15541 ctataattta tatattatgt taaaaggaat actacatagg ctagaccata gtgcttctgc
    15601 tcatgaagtt gtggaatgca ataatgttat gaatatagga gaacacacag aaaattattt
    15661 aatgaaagaa tttggtagtt tgagagaaac tcaatcattt gcccaatcta atagaaataa
    15721 gaatataata ctgattgcat ctacaggaat gggaaaaaca gaaactgcct taatttggat
    15781 agataaagac aaggcatttt ttacacttcc attaagagta agtataaatg cattatttga
    15841 tagagcaaaa aatatagttg gagttggaga atcaaatgat acattcttag gattgttaca
    15901 ttcaacagca atagattatc tggaagaaag taatcaagaa aattcaagtg agattgtaga
    15961 tttagcaaag ttattatcct gtaagctgac attttctaca atagaccaaa tatttaaatt
    16021 tccttttttg tatagagggt atgaaaaagt gtactcaaca ttagcatatt caaaagtagt
    16081 aatagatgag atacaagcat attcaccaga aatagcagct gtattggtaa aaggtataga
    16141 aatgatacat aaaataggcg gaaaatttat gataatgaca gccactatgc caactatata
    16201 tatagatgaa ctaaaaaaaa gaggtgtagt tgacagtaac ttagctaact tgacatgtaa
    16261 cacagaaaaa ataagacatt gtgtgtctat tgtggaaaat tcaatagatg aaaatttaga
    16321 acaaataata caaagtggaa taaataaaaa agttcttgta atagtaaaca cagttaaaag
    16381 tgctatagaa aaatatgaat taatagaaga gataattaag tcaaaaggaa tagatattaa
    16441 tgtaaatctt ttacattcta tgtatataca agaagataga gcaaagctag aaaaatatat
    16501 aaaagaattt gcagatagtg atagtaatgg tatttggatt actacacaat tagtggaagc
    16561 atcccttgat attgattttg atgaacttca tacagaaaat tcaacacttg acagcttatt
    16621 tcaaagattt ggcagatgtt atagaagtcg tgagtatgaa gaaaatagtc caaatatatt
    16681 tatatataca gaaaaagcta caggaatagg tagtatttat gataaagata ttgtagaaaa
    16741 aggacttgag ctattgaaaa actgtatgaa tggaaaaaaa tgttttaaaa tgaaagaaaa
    16801 acataaagta caaatggtta agatactata ttctaaagaa agtttaaaag atacagattt
    16861 tgaaaaaaaa tttactcgtg ctttagatat attagatacg ataacaccat atagtttagg
    16921 ctctaacgag gcacaaaata tacttagaaa tattgaagga tatacagtaa taccggagga
    16981 tatatatagg aaaattgaaa aaactttaat aagagattat gaagagttgg gacaagaact
    17041 tacagaggca tatagcaatg atgatagaca attgataaat gaaataaagt ctaagagaaa
    17101 aaaagcaaga agagagataa ttaaaaaaac tgtaaattta ccaatatata aagctaaaca
    17161 aaatatagta gatatacagg tgaaaggtct ggaagattta aagatacttt tatataaata
    17221 tgatatacat gaagatgtgc atacaaaaca tatatttggc aaaggtgtga ttataggtaa
    17281 ggaaatagac caatttattt gatttttata ttaacaatgt ggctaaaatt tactaaagaa
    17341 ggtgagtttg tgccaataga tttagattta ttgaaagttc aaggtgtaaa gataaattat
    17401 tattatgtat gtaaaagaaa actatggcta ttttctaaag gaataactat ggaacaaaat
    17461 agtagtagag tattgtctgg gaagatagta catgaagatt catataaaag aatgaaaaat
    17521 aaagaaatgt tgattgatga tatcttgaag ctagatataa taaaggacga gtatgtaaaa
    17581 gaagtaaaaa taagttcaaa gatgaaagaa gcagatgaaa tgcagttata ctattactta
    17641 tactatttaa agaatagtat gggtatagag aaaaaaggaa ctataaatta tgttaaggaa
    17701 aagaaacaag aagaattgat attgacagaa gaaaaagaaa ggattattga aaacacactt
    17761 ataaatataa atgaaataaa catgaaatta tctcctccaa aaattgagaa aaaaccttat
    17821 tgtacaaaat gtgcttatta tgaattttgt tttgtgaagg aggagtaaca atgtataaag
    17881 actattatat tataagtaat ggtaatataa aaagaaaaga aaatacaatt tactattata
    17941 atttagaagg tgataagaaa atattaccag tagagcaagt tgatgatatt catatatatg
    18001 gtgaagttga tttaaacaca aaacttataa attacataag tcaatatgga gttatgcttc
    18061 atttttataa ttactatgga tattattcag gtacatatta tccaaggaag aaaaatgtat
    18121 caggattcag tttagtatgt caatcagcct gctatttaga taaaaatgaa agattatact
    18181 tagcaaaatc ctttgtggaa agtgcatcat accatatttt aagaaatatg agatattaca
    18241 aagttgatga aagtttaatt gatgctataa aggatgaact aaatgatttg aatgatgtca
    18301 aagaaatatc tgaacttatg ggtaaagaag gtagaataag aaaaacatac tataaatcat
    18361 ttaatcaaat attaaaagat gactttgaat tttataaaag agaaaaaaga cctcccaaag
    18421 acccagtaaa tgctcttatg tcttttggaa atagcataat gtatactaat gttttaagtg
    18481 agatatataa gacacaactt gaccctacaa taagcttttt acatgaacca tcaacaaaga
    18541 gattttcttt aagtttagat attgcagaaa tttttaagcc aattataatt gacacaataa
    18601 tttttagcct gattaataat aaaagaatta ataagaatga ttttgaatat caagataaaa
    18661 tttgttattt aagtgaaaaa ggaaagaaga aatttttgca agaatttgaa aataagatgc
    18721 agacaaccat aaaacataga aatctaaata gaaaagtttc atatagaatg tttataagat
    18781 tagagtgtta caaattaatt aaacatttta taaatgatga aaggtacaaa gttttaaagg
    18841 tgtggtggta agtatgtttg ttattgttac ttatgatatt gttgaagcaa agtcgttaaa
    18901 tagaattaga aagatactta gaaaatattt gacttggacg caaaattctg tttttgaagg
    18961 caatattact gatggaaagt tacataaatg tatttctgaa atagaaaata ttattgataa
    19021 tagagaggat tcaatctata tttatgagat aaaaaatcct aattcaatta aaaagaaatg
    19081 ctatggtatt gataagtatt ctgatgaaat gtttatatag gtttgcagtg agcgacattt
    19141 atgctaaaat aggtgttaac agttggaata taagggattg aaggtgtatg ataactgtta
    19201 tcaattgcac tactgttcgc tcactgcaaa ttttgatgtt tttattgaat tataattgct
    19261 tgattgaagt attttcaatg tattcaatta cacctatttt ggggttttat attaactaag
    19321 tggtatgtaa agcaatatat tctagcagga gcagatttag gagctttaca agttttatat
    19381 taactaagtg gtatgtaaag attctttata cagagaaaca cgatactatt tacaggtttt
    19441 atattaacta agtggtatgt aaagatatct ttgtactctt gattttccag ctttgatata
    19501 gttttatatt aactaagtgg tatgtaaaga attgcataat attagagagt ttaataccat
    19561 caagtgtttt atattaacta agtggtatgt aaaggatggt aaaatgggag atactttctt
    19621 tggattaaca ggttttatat taactaagtg gtatgtaaag ttgcttatac ttacaacttt
    19681 ttctgctgta gcatgtttta tattaactaa gtggtatgta aagaaaactg tttctacttt
    19741 tttagtccca agtaattccg ttttatatta actaagtggt atgtaaagcg catttttgct
    19801 tttatattgc ttcttctatc ttctagtttt atattaacta agtggtatgt aaagtctaaa
    19861 atatcctcac aactcctgta attgtatttt ctgttttata ttaactaagt ggtatgtaaa
    19921 gtggtttact aagaaaataa cggattttta agagaaaagt tttatattaa ctaagtggta
    19981 tgtaaagttt gatgaaaaag aagaatataa gagggatgtg gcaagtttta tattaactaa
    20041 gtggtatgta aagtaactcc aaactccgta agtcttgcca tttctccaag ttttatatta
    20101 actaagtggt atgtaaagga gtatcaagag accagggaaa ggcaggtaaa gcaggtttta
    20161 tattaactaa gtggtatgta aagaatggta aaatgaaggc ataataaatt tcaatatagg
    20221 ttttatatta actaagtggt atgtaaagag agacagttta aaactaaatc agaagaaaag
    20281 tcttatagtt ttatattaac taagtggtat gtaaagttta aagcaccctt aaaatctggt
    20341 tgggcatgtt ctcgttttat attaactaag tggtatgtaa agttggtaga caacctggta
    20401 cttggcttaa gattgataag ttttatatta actaagtggt atgtaaagca tgtgatagag
    20461 ataatataca atatgataga gaaagtttta tattaactaa gtggtatgta aaggcataaa
    20521 ataccttcaa aaatagcttc tactgacagt tttatattaa ctaagtggta tgtaaagcga
    20581 aataatgttt tgttttcgat gggtatatat acgggtttta tattaactaa gtggtatgta
    20641 aagtagcttt tgcgactgga agatacgcag ataatttagt tttatattaa ctaagtggta
    20701 tgtaaagatt gtaagctctt taatttctaa attagctccg attgttttat attaactaag
    20761 tggtatgtaa agttaagtag agcaatgtat catggttatg tgaaaagtgt tttatattaa
    20821 ctaagtggta tgtaaagcta gtaaattttg ctaaaaggca ctattattgt agtgttttat
    20881 attaactaag tggtatgtaa agcagttgac acaatgagta aaagcgaagc agagcaagat
    20941 tttatattaa ctaagtggta tgtaaagtta tgttatagga gcaggttgca agaagtataa
    21001 gcaagtttta tattaactaa gtggtatgta aagcatagat tttggagatt taagggaagg
    21061 caatatgtgt gttttatatt aactaagtgg tatgtaaagg ggtttgcatc tttattagat
    21121 aattttatta atagagtttt atattaacta agtggtatgt aaagtaaata tagaataaag
    21181 cattttagtt tacaatgcaa gttttatatt aactaagtgg tatgtaaaga cctaaaaaat
    21241 caatgcagct tatcttatat gcaatggttt tatattaact aagtggtatg taaagattaa
    21301 ggaatagtaa ttacagtgga ctggagttag tgttttatat taactaagtg gtatgtaaag
    21361 acgtttcgca agggattttt taaagcgaat ggataagttt tatattaact aaatcatatc
    21421 taaataaaat acacttacct ataaacatga taaaatcaat acaaaaatga ggtgaaacaa
    21481 aatttatgat aaggaaatta aacaataaag atataaataa aatcatggaa atatgggaag
    21541 aaagcacaat caaagcacat ggctttataa gtaaagaata ctggcaaaat aaccataatg
    21601 ctgttaaaaa tgagtatata cctatatcag atacatttgt atatgatatt ggagatgaaa
    21661 taaaaggatt tataagcata atagataaaa gttttatagg agctttattt ataaagccca
    21721 aataccaaaa tctaggtatc ggaggtaaac ttttagatta tgcaactgaa aaatataaaa
    21781 gtctaagttt agcagtatac aaagataata aaaaagcagt tattttttat aataaaaaag
    21841 gttttaatgt agtaaaaggg caattaaatg aagactcagg atttaaagag tacataatgg
    21901 aatatagtaa ataatatgag tgtattctat taaaaagata attaatctat ttgatatttg
    21961 attagaggca tttttataag agtaattttt ttaatagatt atataaagat aaatatattt
    22021 gttatatatg taagttaatc agaattgcta tggttacaaa agaggaaaat aaaattgtat
    22081 ggtgtatttc aaatttaaca aatttaccat ttaaaaataa taagtttgat ataatattaa
    22141 atgtactttc acctgtaaac tataaaagat ttgaaagagt tttaaataat tatggaatgg
    22201 caagaaaggt ttgttaaaat ttaataatta ttagattata gaaagttgcc ataaaattag
    22261 ttcagtaaga actagtttca tggcaatttt aattcttatg agagtattta tttttacata
    22321 ttttaaaaac ttatacagta taataatgta atcttactat atcagtctat agatatttaa
    22381 atcttaaaat gttctctttg agataagttt atttcagagt cacaatttcc acaacagtaa
    22441 gatgttgaga catctttaaa tttctcagta ttctcatcaa agttcatttt tttctttgca
    22501 ataattccta ccttggttaa attagagcca caattattac agttttgaat tttgcatagg
    22561 ttagatagtt tatcaagtgt aacatcttct aataattcat cacaattaca acatctgata
    22621 gaagaaactt cattagatat ttcaataggt tcagcttctt tagaagaaaa tttatatgtt
    22681 atgtcatttg atactttata acctgtgtca aatatattag aattacaact tggacatagg
    22741 tactttggaa gatatatatc ttttttttca aaagtatcac tactagtatt atagtttaat
    22801 tcatctaaaa ttggtctttc atctaggtta ttatctttat ttaagtttag aagataatta
    22861 taaatttttt tatcttcatt ctgttttgat gtatccaaca agtgttccat tatattagtg
    22921 tattttttat tttttgaagg catgtttaaa tccagataat tgttttcaat agcaaagcac
    22981 actatattga aatagttata tttaactgca aaaaataata acatttcatc aaatacttta
    23041 tctattattt tgtcagaaat acttatccaa aaatctggtg actctaagta aactttccta
    23101 ctaagttcat ctaactcttc atctatatta ttttcattaa ctttcatatt atcacttcct
    23161 tttaaatatc ttgtgtataa actatttgtt actgtttttt atataaaata aaatcttata
    23221 taaaaatata tcatattata atttacaagt atagtctaaa tagagctgtt tttaagcaaa
    23281 gtcttataaa tgactattaa aacataaagt aacagtgtaa agtttactta taaattaaat
    23341 gtaaacctaa atacttataa gggttgacaa aaaaacgcct ttattttata ctaaaacaaa
    23401 gctttaatag aagctttgta tatgtacata taaactaaat tgaataagac atatgaaggg
    23461 aattataaaa atgaaaaata ctaaagtaaa tattcaagat ttaactaaaa tgtcaatatg
    23521 tgtagcatta ttgtgtatat catcttacat atatatacca ttgccattta ctccagcagg
    23581 agtaacagca caaacaataa tgataaattt gatagctttg attttaacac caagacaagc
    23641 atttagtaca gttggtgtat acataatgat aggtttaatt gggattccag tatttggtgg
    23701 aggtacatct ggtattggaa agttgttaag cccaacaggt ggtttttatt ttgggttttt
    23761 gtttgcagca ctaattatta gtttattaaa aggacgaaat aatgatatta agagatatat
    23821 actaataaca atttttgttg gaatgcctat tatatatttt atgggaacag tgtttatgtg
    23881 ctattttaat caaatgacag tagaagcagc actttttgca gcagtagtac catttttaat
    23941 tggagataca ataaagtcag ttattgcgtc ttttttaggt actaaactga acaatgtact
    24001 cagaaaaaat ctataatata aggaagggtg taatatatgg aggaggtttt tatattagga
    24061 ggattgagaa gtcatatagg tctaaaaaat ggaatttttc aatttgttca acctgaatta
    24121 ctaggagcaa gtgttttaaa acatttaatt aagaagtatg aaattgataa aattgatgag
    24181 attatatgtg gaaatgctgt tgggacagga gggaatatag ctagattaat gactttaaca
    24241 gcaggagtgt caaatgaagt gcctgctttt actgtggata tgcaatgtgc ttctgctatg
    24301 atgagtatag acatagcttt ttcaaagatt aaatctgggc agtgtgattt aatcattgca
    24361 ggtggttttg aaagtagttc tctacaacca atgcgtacat atcataagaa tgataagcga
    24421 tataatataa ataatccaaa ttatacagtt gctcaatttt ctcctgatga taatagccaa
    24481 aatagtatgc tagaaggggc agaaagagta gcagagttat atcaaattaa gaaagcagaa
    24541 ttggattttt gggtaaagga aagtcataaa agagcaaaag aagcaagaga agaaaaaata
    24601 tttgatgata ttatatttcc tataaataat agtacatgtg atgaaggtat acgtgataag
    24661 atgagtcaaa gattattaga tagaatgcca tctattttag gaaaagaaag tattacgaat
    24721 gcagcaaatt catgtcttat taatgatgga gcttcattta ttatactatg ttctaagaag
    24781 tatctagaac atgcaaaaaa taagcctata gcaaaaattg ttaatacatg tacaataggg
    24841 acagatgcaa atttaagccc aacatcagcg ataaaagcaa tggataaact tttagaaata
    24901 gaaagcctta gttatttaga tgtatctgct gttgaattta atgaagcatt tgcagttata
    24961 gatgtgctgt ttcaaagaaa gtatccagag ttaatagata gatataatat atttggtgga
    25021 gctttagcgt atggacatcc ttatggtgct tcaggtgcaa ttattgcatt acatctttta
    25081 aaagcattag aaaagactaa gggtagatat ggaatttgct ctatagctgc agctggtggt
    25141 ctaggttcag ctttaatgat tgagagggtt taaattatga actattatga acttttaaga
    25201 ataaaaagcg ataggcataa aaataaaaat tttttaatta tagatggaga aaaatatact
    25261 tatgaaaata tattgagtta tagtgaggaa ataggaaaac aaatttattt aggtgaaaat
    25321 attcctattt taatatattc taaaaatata atgtttcagt taattagttt ttttgctatt
    25381 aattattcaa aaaatattcc tattatttgt cattataatc tttctaaaaa agtattagat
    25441 gatattttac taaaaaataa tatttcaatc attatatctg atgaatctat ggacgttatt
    25501 gatttatatg atgataacaa taatgaagta gcaaaaaaag ttaatttgtc tatttttaat
    25561 cctaaattca atatatatat gtatcaatat aagaaactta taaattattt agataaaagc
    25621 atttgtatgg gagctttatc ttcaggttct acaagtgttc caaaagtatt atatagaacg
    25681 tatgagagtt gggctggatt ttttcctata caaaatgcta tttttaatat ctctggggaa
    25741 tcaatcttgt ttattaatgg aacacttagt tttacaggga atctaaattc tataatgtca
    25801 gtattatatg aaggaggtag tattgtaata agttcaggtc ttaattgtaa atcatggatt
    25861 aggacaataa aacaatataa tgtaactaac atatatctta tacctataaa acttcaactt
    25921 ttggttaaac acttaaaaga atcagtactc aaagttgtaa gtatttttac agggtctcaa
    25981 ttattatttg aagatactgc aagaaatcta aaaaaatata tgccaaagtc tgaaattatt
    26041 ttatactatg gggcaagtga attaaattat attacatatt tatgctatga tgaactaatt
    26101 gaaagacctc taagtgtagg cagaccattt ccaggaatag atgtatatat tagagaagaa
    26161 aaaatttttg tgaatacaga atatgcagta tataatgcaa ccaaacctta ttctgtcaat
    26221 gatattggat attttgataa tgatggatac ttaatatttg aaggaagaag tgatgacatt
    26281 gtaaatattg gaggatttaa agttagttct actaagattg aaaatgaagt taaaaaaatt
    26341 cctcaaattg aagatgcagt tgtattgccg tattcagaca ctataagagg aaatcaaatt
    26401 gtattatttg taataactat agacaaaatt acgaagaaag atttgttgat taaaatgaaa
    26461 gataatctaa tgaaaaatga aatcccaaag aaaattatct ttttaaattc atttccatat
    26521 acctcttctg aaaagataga tagattagca ttattaaaaa tgttataagt ataatattta
    26581 gtaattttag tgaaccaatg tattgataaa aacatattgt atatagtaaa ggaggcttac
    26641 aatatgtcaa atcaatacga tataactgtt attgggggag acctgcgaca agtgtacatg
    26701 gtaaaaaaac taattaataa agggtattct gtagtcactt atgggatatc aaatgaaatt
    26761 ataaatggaa tcgttggaaa atcaacttca ctagctgaag cactatccaa aagttccatt
    26821 attttatgtc cgattccatt tacaaagaat caagttaaca ttgaaaatac tgataattgt
    26881 cttgatgcta ctattgataa cttgctaatt aatttatctc ctaatcaaat cttatttgga
    26941 ggtaacatac caactaaagt ttgtaaattt tgcgagcaac actctattca gatttatgat
    27001 tttatgaaaa tgaatgatgt tgctatttta aatgcagttg ctacagcgga aggggctata
    27061 tcagaagcta ttaaaagaag cataatcaat atacatcaaa gtaactgctt agtattaggt
    27121 tatggaagat gtgcacaaat acttgcaaaa aaattatatg ctttggatgc aaatgtatgt
    27181 gttggtgcaa gaaataatga tgcaagaagt acggctagtg catttggata ttccagtatt
    27241 tctttaaatc aacttgatga aaaactttta caatttgatt atatttttaa cactatacca
    27301 gattgtattt tgactaaaga aagattagaa aaagtatcac aagaagtaac tattatagat
    27361 atagcttctt ctcccggagg agtagattat tctgctacta aagaagtagg gattaatgcg
    27421 actctttgtc ttgggcttcc tggtaaatat tcaccaaaga catctggtga aattcttgtt
    27481 aacgctatag aaaatattat aaatgaaaga agggattaga aatgagactt gaaggactta
    27541 caattggagt aggatttact ggttcgtttt gtacttatga taaaatattt atagagttag
    27601 agaatttggt taaagaagga gctaatgtcc acactatatt ttcagatgta tcacaaaata
    27661 tagattgtag atttggaaat tctgaagagt ttatgaaaaa agcatatgaa cttacaggaa
    27721 ataaacctat agttacgatt gaagatgcag agccatttgg acctaaggga attgctgata
    27781 ttataataat agctccttgt actggaaata ctgctgcaaa attagcaaat ggtattacag
    27841 attcaccagt attaatggca gcaaaaggtc atcttcgtaa tgataaacca ctagtaatat
    27901 caatatcaac gaatgatgct ctaagcttta atttcaaaaa tattggtatt ctccttaatt
    27961 ctaaaaatat atattttgta ccttttggtc aagataattg caaagcaaag cctaattcta
    28021 tgattgcaca tactgagcta ataattccaa ctatagaact tgccctagag aataaacagc
    28081 tacagcctgt tataaaaagt ccacatcaat agaattggtt ctataatatt atgtattaga
    28141 tgcaagacta gcaaagaggc tgtctcaaaa tagagataag cctctttttc attgttgaaa
    28201 tgatgaaaat atatttaatt tcatacaaaa aaatgcctta tggactaaaa agtctatttt
    28261 gagctaatct ccataatttt tggtatatta aaattgttta cttaatctaa tttttaaatt
    28321 agaatttata tcgctttgac tcttcaaata tcaatctaac tgttgttgat acaaaagaag
    28381 aggacataat taaaacaaat actgatagtc ctctaataaa caaattattt acataataag
    28441 ttactattcc aatacctcca atcgttaaaa ttacacttat aatcaaagaa aaaatgctaa
    28501 ttatttttaa ttttttcatg attatcctcc ttcttataaa tttcaaattt atctatataa
    28561 tattaaatat attttatttc atagtatata aaaatcatga atatatattt ttttaattat
    28621 actatatatt gtaacttttt gtatatttaa ctatatttac tttaacttgt taatcaagtt
    28681 attattttca ttttcaagta tttttatttt taaacttgat acagcaccat attaaaaatc
    28741 atttttataa agttttattt gtaaaaaata caaaaatata aacgttaaaa aaataacgtt
    28801 atccttaagt ttttagatga ttttattagt aatttacgta aaaaataaag tgacatctag
    28861 ttaaattaag tatttgtaaa gctagatatt attagtataa atgatgttat attagataaa
    28921 aaataaaatg ttaaaaaaat aacttgaaaa tagaaaaagt gaatgctata atgttaataa
    28981 attaacataa taaataaaaa actatctcag gaggttgtta aaatggctca taaagaacaa
    29041 gtaataatag aaaataaaaa agaaaatgaa gttatgtcag ttggtttaga tactgtaaat
    29101 aacgttgaaa gcttagttaa aaaactagca aaaatcaaag aagctcaaaa aatatttgct
    29161 acatatactc aagagcaagt agataaaatt tttttagcag catctttggc agctaataag
    29221 caaagagttc ctctagctat aatggcacaa aaagaaactg gtatgggaat tgctgaagat
    29281 aaggttataa agaatcatta tgcctcagag tatatatata atgcatataa agaaactaaa
    29341 acttgtggtg ttatagagaa agatgaagca tttggtatga ctaaaattgc tgagccaata
    29401 ggtgttattg cagcagttgt acctacaact aatccaactt ctactgctat ctttaaagca
    29461 cttttagctt taaaaactag aaatggaata attttctctc cacatccaag agcaaaaaat
    29521 tctactatag aggctgcaaa agtggttctt gaggctgctg ttttagctgg agctcctgaa
    29581 ggtataatag gatggataga tgagccatct ttagaattaa ctacaactgt tatgaaagag
    29641 gtggatttga ctcttgctac agggggtcct gggatggtta aatcagcata ttcatctgga
    29701 aaaccagcta ttggagttgg tgcaggtaat actccagcaa taattgatga aagtgcagat
    29761 ataaaaactg ctgtaaactc aattcttgta tctaagactt ttgacaatgg tatgatatgt
    29821 gcttctgaac aatcagttat agtattggaa aatatatata atgaagtcaa aaaagaattt
    29881 aaagagcgtg gtgcttattt attagataaa gatgaaacag aaaaaataag aaatataatt
    29941 ttagtaaatg gaggattaaa ttctaaaata gttgggcaaa cagcttgtac tatagcaaaa
    30001 cttgcaggat ttgaagtccc agtagatact aaggttttaa taggtgaagt agaatcggtt
    30061 gaaatagaag aggcttttgc tcatgagaaa ctttcaccag tgcttgcaat gtacaaggca
    30121 agtgattttg atgatgcagt tagaaaagca gaaaaattag tagaagatgg tggatttggg
    30181 catacatctt cattatatat tgatgatgta aatcaaagag aaaaacttga taagtttact
    30241 tcagctatga aaacttgtag aattttaatt aatactcctt cttctcaagg tggtataggg
    30301 gatttatata attttaaact agctccttct cttactttag gatgtgggtc ttggggtgga
    30361 aattcagtat ctgaaaatgt aggagttaag catttactta atattaagac agtagctgag
    30421 aggagagaaa atatgctttg gtttagagca ccagaaaaag tttatttcaa gaaaggttgt
    30481 ttaggagtag cattaaaaga acttaaagat gtaatggata agaagagagc ttttattgta
    30541 acagatacat tcctttacaa taatggatat actaaagcag ttactgactt attagatgaa
    30601 atgaatatta agcacactac attctttgaa gtagaaccag acccaacttt ggaatgtgct
    30661 aagataggtg ctaaagctat gagagaattt aatccagatg taataatatc tataggtgga
    30721 ggaagtgcaa tggatgcagg aaagataatg tggacattgt atgagcatcc agatgtagat
    30781 ttccaagatt tagctatgag atttatggat ataagaaaaa gagtatatac attccctaaa
    30841 atgggtgaaa aagctaactt tgtagctata ccaacatcag caggtactgg ttcagaagta
    30901 acaccatttg ctgttataac tgaccaagat acaggggtaa aatatccatt agctgattat
    30961 gaattgatgc caaatatggc tatagttgat tctgatatga tgatgaatat gccaaaaagc
    31021 ttaacatctg cttctggtat agatgcctta actcatgcat tagaagcata tgtatctatg
    31081 cttgcaaccg attatacaaa tggattagct ttacaagcca ttaagagtat atttgaatat
    31141 cttccacgtg catatgacaa tggtgcaaaa gacccagaag ctagagaaaa aatggcaaat
    31201 gcttctacaa tggcaggtat ggcttttgca aatgcatttt tgggtgtttg tcactctatg
    31261 gctcataaat taggtgcttt ccaccatgta ccacatggtg ttgcaaatgc tcttttgata
    31321 acagaggtta tgaaatttaa ttcttcagac gcaccaaaga aaatgggagc attctctcaa
    31381 tataaatatc cagaagcatt aaagagatat gctgaagtag cttcattctt aggattaaaa
    31441 ggaaattctg aggaagagaa attccaaagt ttattgtcag ctattgaaga tttaaaattt
    31501 aaagttggta ttcctaaaac tataaaagaa tttggagttg aagaatctaa gtttatggac
    31561 tcaatagatg aaatggtaat tcaagcattt gatgaccaat gtacaggagc aaatccaaga
    31621 tatccactta tgaatgaaat aaaagatatg tatttaaatt catattatgg aagataaatt
    31681 tactataaaa taggaaaaat aaaaattctt atacataaaa caaagtttta ttgtataaga
    31741 attttttttg attggaaaaa tgtatagttt ttattcttta atttaaaatt caattaaata
    31801 gtgttattat aataataaaa gaatgttttt atggttatta ggttgatttt atgtgtaaca
    31861 tttaagaata gttattacaa tataaatgat gaattggtat aaaaaggata tattatctat
    31921 atgaagtatt gactaattat gggaggagtc gaatgacaag aagaagtaag agttttacat
    31981 taattataat tctttttttg acatcattat ttttgacaat gggatatatt tatatagaag
    32041 atacaaaaaa tcttcttatg tcagaagcaa aaatacatat aaaagaagtt gcaatacaag
    32101 gctctcaact agcacaaaga cagattgaaa aagatttaga tgttttaaat attttttcta
    32161 actattatgc ttcaaatcca gacataccta atgaggaaaa aatgaagaat cttttggatg
    32221 agttggaaaa tcaaaaattt tacactatgg caatagttga tataaatggt aatgctgaaa
    32281 ataccaaagg tgataagttt tcagtgaaag atagagaatt tttcaaaaac tcaattaaag
    32341 gtaaaaagta tgtttcaagt ccatatgctg atgaagtaaa taagtctata aagaagatta
    32401 ctatttcagt gccattatta aataatgata aagtagttgg agttttatat tgtacctaca
    32461 atatcaatac tttgatgaaa cttattaatg tatcttttta tgaaaataat agtatatctt
    32521 atgttgtaaa aaatgatggt acagttattc ttcatccaca agaggatagc atatcaaaaa
    32581 atatttataa attgttaaaa caggataatg atattcgaga agtaaatagc ttaaaaaaag
    32641 aactgcaaga aaacaaaaca ggtgcaactg tattaaagat gttggaagaa agaagatatt
    32701 taggatatgc tactatggat aatgggaata gtgaaaataa ctatataaaa gattggaatt
    32761 tgattttttc aattccagaa actgttgtgt ttagtaattc agaaaaaata atcaacagag
    32821 ctatatatgc agtgttattt attatattaa tatttattgc aattattttt tatataattt
    32881 atataaaaaa atctaatgaa aaagaaatat taagtttggc ttatgaagat aaagttaccc
    32941 atataggaaa tcaaaataaa ttttatagag agtgtagtaa gtatctttta gataaaccat
    33001 ctttaaatta tattatagta tattttgata ttaataactt taagatgatt aatgatactt
    33061 ttggatatga atttggtgac aacttattaa ttacgatagc aaaagcttta aaagaagaac
    33121 tgacagaagg ggaagtattt gcacgacttt caagtgatta ttttgcaatt ttctgtgatt
    33181 ataaaaatgg aagaaataaa attataagaa aacttgatag tatacgaaat aatattgaaa
    33241 gtaatttgag tatagtcttt gaaatttctc tgtgtgtggg tatttatttt gttgaagaag
    33301 atgaagtaga tattcaaaag gctgtaaata aagcaaatat ggctaggtct gttgctaaag
    33361 gtaaaaatat aaattatgct atttataatg aagatgttag gaataagctt agtgaagaat
    33421 caatgatttt agatgatata aaaatagcat tggtaaaaaa tcaatttgaa gtgtattatc
    33481 aacctaagtt ttcacttgta aatggagaaa tgatagggag tgaagcacta ataagatgga
    33541 atcatccaga acatggtttt ataagtcccg cagtatttat tccaatagca gagaaaagta
    33601 agttgatttt aaaaattggg agatttgtat ttgaaaaggt ttgcactgat ttatctgaat
    33661 ggaaaaaaca aggtaaaaag atagttccag tttcagtaaa tttatctaga gttgaattgt
    33721 atcaaccaga tattgtaaaa tttatcaata aaacaataca aatgtataat ctaagttcag
    33781 atttaattga aatagagata acagaaactg tggctattaa tgagttaaat attttaaaga
    33841 atgttttaaa tgagttgagg aagtatggat tttctatttc tatggatgac tttggtacag
    33901 gatattcttc tattagttgt ttaagagata tgccaattga tatattgaaa ctggataagt
    33961 cttttcttga tggtattgaa catgatgaga gaagtcgcaa tatagcaaaa tcaatagtgt
    34021 ctctagcaaa atcactagat ttagttgtaa ttatagaagg tgttgagagt aaagaacaag
    34081 ctgagttaat gaaacaattt ggatgtgatt tagttcaagg attttatttt gcaagaccaa
    34141 tgccagctaa gaatttttta gatttacttt gaaataaaat ttgattttta tataaagcac
    34201 tattatgaat gcttgatttt aataaatcac tgctaaataa ttagtgtttt tttattttat
    34261 caatgtatta tctaatataa ggtaaaaaat aattgtattt tataaaaatt atattaaaaa
    34321 taaatttatt ttgctaataa acatgtcatt aacaaaagaa tagtactata atgtatagat
    34381 gataaaaact ttaataaaaa tttagtgtat aggagttgtt taagtatgat agatgtaagt
    34441 attgataaaa aacttaagga aagatggcct aatgctaagc tgggttgtat tcaagcaaaa
    34501 gtagcagtaa ataaaagctc agaaaaatta attaatgaaa taaatgagtt ttgtgatact
    34561 ctaaataaaa atctaaaagt tgaagacatt acaaaactag ataaaattaa agatgccaga
    34621 aatgcatata aagaacttgg aaaaaatcca agtagatata gaacgtcttc agaagccttg
    34681 gttagaagaa ttattcaagg taagggtctt tacacaataa acaatatagt agaaataaac
    34741 aatttaatat caataaaatc attatatcca gtaggtacat ataatgtaag taaattacat
    34801 tcaccagtat gttttactgt aggagatgaa ggagaacagt ataagggtat tgggaaagaa
    34861 ttaataaata tagaaaatct tcctatattg tctgattcag tgggtaaatt tgggagtcca
    34921 actagtgatt ctgaaagagc aatgataaca gaagatgcga atgaaatatt aatttgtata
    34981 ttttctttta gtgaagaatc tgatattgaa gaatattttg gatatgctaa ggaattactt
    35041 aaagaatacg ctgatggaaa agatatagaa actaaaataa ttaaatagaa tccatataac
    35101 aacgttatgt taagctaaaa ttcaatataa aaataagtta tgataaagaa actaacacaa
    35161 aattagtttc ttttttgaaa gagtagttta gtgttacaaa aatagataga tatccccacg
    35221 agtattaaaa gtatggtaat atattaatat agaagggatt gtatttaagg gattttgaaa
    35281 ggagtgattt ataataactg ttttttctaa aagataaaat ggttttatgg aaataactat
    35341 atatttaaaa atataaataa taatacagtg atttgggtat ataaaataag acatacatgg
    35401 ttttgtttaa aaccaataaa gtacataggt aattacaatg taaggaagga tttaattatg
    35461 attgatggaa aagaggtaaa acaagaaagt ggacaaaaaa atctaaaatt taagatagct
    35521 atagtagtag gtattttatt tgtagcttat ataggaatgg caatcttttt tagaaatcat
    35581 ttttactttg gaacgactat taatggtgta agggcttcag gaaaaacagt tgaacaagta
    35641 aatgatttgt taacatctag tactaattcc tatactttaa acttagatga aagaaacaat
    35701 aagaaagaac aaataaaagc aaaagatatt gatttaaagt ttacaccaga tgataaagtt
    35761 caaaagataa aagatagtca gagtgctatt ggatggattg gtgggatatt taagtctaaa
    35821 gagtataaca atatggtaac tctttcatat aataaagatt tactagaaaa aagatttaac
    35881 gctctatctt gccttgatag taaaaatgtt gtagagccta agagtgctta tcctaagtat
    35941 gatagtacta aaaatgctta tgttatagtg ccagaggtac ttggaaacaa agtgaaaaaa
    36001 gatgattttt ataagttagt acaaaactca attaattctg gtaatacttc tatttcttta
    36061 gatgaaagta aagtgtatga agtgccaaat aacacaaagg attcagaaaa gttgaaacaa
    36121 gctattaaga cactagataa gtatgttggt gtagttgtta catatgattt tagtgaccgt
    36181 aaggaaactt tagatggttc tgagattaat aaatggttaa aggtagactc agaaaagaat
    36241 tatgatataa ctttcgataa tgatgctatg gtagagtata caagaggttt aagtaaaaaa
    36301 tatagtactt ttggagattc aagaccattt aaaactgcaa caggtaaaaa tattacaatt
    36361 tcaggtggaa tttatggatg gttgatagac aaaaagaaag aagctgaagc attagttgat
    36421 gttgtaaaag ctggtaaaaa tgttacaaga gaacctattt acatacaaaa agctgtatct
    36481 cgtaatagag atgatattgg aaatacatat gtagagatta gtctttctaa ccaacatatg
    36541 tggttcttta aaaatggaga aatgcttgta tcaacagaca ttgtaactgg taatttagct
    36601 agaggatttg ctacaccatc aggtatatat cctcttaatt ataaggctag aaatgtttca
    36661 ttaactggtc aaggttacag ttcccctgta agttattggc ttccatttaa tggaaatata
    36721 ggtattcatg atgctacttg gagaaattct tttggtggag gaatatataa gtcttcaggt
    36781 tcacatggat gtgtaaatac acctttttct aaggcaaaga aaatatatga aaatatagaa
    36841 ccaggtacac caattatttt atactagtaa atgtgttact aacagttaag cgaagtcatc
    36901 ttagtttatt tagggtggct tcctttttat ataaagttta ttgaagttta cataaagact
    36961 atttaaaagg agaaatacat aatgaatgaa aaattaacta agattttaga gaaagctaag
    37021 aatgatgatg aatttagaaa agaacttata aaatcaagag aacaacgtga tgttatggat
    37081 tcattttgta aggttgttac atctagaggt tatgatataa cagtagggga acttttttcc
    37141 ataggagaag aatatacatc aaatctactt aaaagtgtga atggaggggc aacatatcct
    37201 attgaagatt ggagtgattg gtacgaggat ttttttataa tgttgtcgaa tatatagagt
    37261 aactaatgtt attatctact taagattaac accttaattt taaaaaaaac tattagagct
    37321 aagtgtaaat tttatcaatc tttaaaaata ttgatttatt tctagctata tataaaaaaa
    37381 ttagattaaa aataaggtga caaaaatcga tataccttga tgatttataa aagttagaca
    37441 aaatagtgct agatatttct aaaaattcaa aaaaaacaat aaacttcaag agctagaggt
    37501 tgaaaaataa aataaaaaac ttttatatgt aataaattaa attgaaatta taaaaaaaat
    37561 aaaaaagaaa ttttaaatgt cgacatagcg ttaattttaa gacaaaataa cgctattgac
    37621 aaactatatc gctttaaatt ataattattg aaaataatgg tatttaaaaa tcatactcat
    37681 gcaatagtct aaaagtaagt caaaaaaaaa tgtaaatcta ggcaagtact attctaaaat
    37741 atgttttatt aagaaataaa cgttgaaaaa tattaattat tatataaaaa tgctgtttat
    37801 actttaacaa tgaggtagta atctaattag aataaaaaaa tctaattggt tatatacata
    37861 gtaaaaaggt aagagaggtg atttggatgg cttttgaaat aataaaaagc attgttgagg
    37921 cagagcagac agcagacagt atcaaagtaa aagctgttac tgatgcagag tctatcagag
    37981 ctgatgctgt aaacaaatgt gaaagcatat ttgctgatgt aaaaaaacaa gcaaagctta
    38041 tggaagaaac tcttattgag aaggcagtca ccgacagtag agcagaggtt gataaaatct
    38101 tagctaatgc taaaagtgaa tgtctgaaaa ttgaaaaaac tgctaaagaa agaaaaagta
    38161 aggctattga agcggttatt ggaaaggtag tgagataata tggcaatagt taatatgaac
    38221 aaaatatcta ttgttggctt agaagctcaa aaaagccaga ttttaaagct acttatgaac
    38281 cgtgggtttg ttcagattga tgatagtgcc tttcttactg aggaggagga attaaaaaat
    38341 tatcttgtaa aagatagtga agaatctgaa gttataatgc tagagcagaa gatgtctcat
    38401 gtaaaccaag caattaatat tgtttcttca ctagtgaaac aaaaaaaatc cctacttgca
    38461 ccaaaaagag agtttaacaa aattgatagt gaaaaagaag agcaagttta tgctgaagct
    38521 cttgagttaa acaatttaca caaggagata ggtacaataa ggtctaacat aaacttactt
    38581 aacaacaata aaagtatgtt agaaccgtgg gtaaactttg atattcctct tgaaaatatg
    38641 gaaactaagt atacaaaaac tatacttgga actgtaccca taagtgcaaa cattgatggt
    38701 ttaagagtaa aattagaaga tgaaatacca gaaagtgtta ttgggattgt caattctgac
    38761 aagttaatga actatgttta tttaatcact ttaaaagaaa cttttgatga tgttttagaa
    38821 gttttaaaag aatttaattt ttcagtggtt tcattgccaa atgaagaagg tacaccgtta
    38881 caagctatca agaaatacaa tgaaatgata attagacagg aaaacgtttc taaagacaaa
    38941 acagaggaaa ttaaaagaca tgcagagagt atacacatgc ttgaaaatct atatgatttt
    39001 tacactatag aaagagacca aaaaaagatt atagagagac ttgtcaaaac aaaaacaaca
    39061 ttctgtctta atggttggtt accaagcaaa agggcagatg agctaattaa agaaattaca
    39121 gaaaagtttg attgttgcgt tcagactgaa gaaggaagta aagaagaagg cttcccaatt
    39181 ttgcttgaaa acaataaact agtaacacct tttgaaagtg taactaacat gtacagctat
    39241 ccaagtacaa aagatattga cccaaataca attttaacat tcttttttgt tgtattcttt
    39301 ggaatgatgc taagtgacgc tgcatatggg ttaataattg cactagtctg tggatttgtt
    39361 gtatataaat taaagataca aaaaggtgaa ggaaatttaa ttaaacttat tggtatctgt
    39421 ggagtttcaa ctactgtatg ggggcttata ttcgggggtg ttttggggga tttaattcct
    39481 ataaaagcat taattaatcc tttagaggat gttatgacac ttatgggaat gtcgttactg
    39541 ttcggtatta tccatatata tgtaggtttg ggaataaaag catatacatt aataagaagt
    39601 ggaaaggtaa tagatgcaat ctttgacata ggtttttggt atttgtgtat aacaggtgta
    39661 tgtctactta taataccttt tgttgcaggg gacataggtg tattttcaga agtaggaaaa
    39721 tatttagcaa ttataggtgc tataggtctt gtacttactc aaggaagaag ctttaaaggt
    39781 attcctgtta agctttttaa aggattttca agtctctatg gcataacaag ttattttgct
    39841 gatatattat cttatacaag aattatggca ctttgtttaa caacaggagt tatagcacaa
    39901 gttatcaacc ttttaggagc tatagctggt ccgatacttg cagttgtaat cggtgttgta
    39961 ggacacacaa taaaccttct tataaatgca ctaggtgcat atgttcatac aagtaggtta
    40021 cagtatgtag aatttttcaa taagttttat gaaggcggag gagttccttt tgttccgttt
    40081 aagtataaaa caaaatatac aagcattaat aaaaaggaga tgtaaatatg gaatttttaa
    40141 acgcgttagg aaatggtcaa tttttggcaa tatcaggagc tgctatagca gcattatttg
    40201 caggtatggg ttctgctaaa ggtacttcaa tagcaggtca agccgcagca ggtgttgtaa
    40261 cagaagaccc tagtaaattc ggtcaacttc tagttttaca acttcttcct ggtacacaag
    40321 gtttgtatgg atttatcata gctttcctta ttttatcaaa agcagggatt attggtggag
    40381 atgcaatacc aacatcagct caaggtttac aacttcttat ggcaggactt ccaattggtt
    40441 tagttggttt agtatcaggt atagcacaag gtaaagcagc agcagcaggg gttggaatag
    40501 ttgcaaaaag acctgaagaa cttggtaaag ctataacatt tgcagcaata gttgaaacat
    40561 atgctctttt aggactactt gtttctttct tagcttacaa tggaattgca attggttaat
    40621 tatagattat aattcataag taaaaaaaat gaaaaggagg aagcactcct atgggtaatg
    40681 aacaaaaaat gattgacaga attatagctg atgccaaaca agaagcacaa gaaatattgg
    40741 ataaggctaa aagtgaggca gatttaaaag taaattcagc taatgaaaaa gctgaaaagg
    40801 aaatggcttc ctatacaaag cttgcagagg cagaagcaga aaaagcagct tctaaggaaa
    40861 tatcaggagc ttatatggag gctaaaaaac aaatattatc aaaaaaacaa gagattttag
    40921 aagaggtaat tttagaagct aaaaacaaac ttctaaatct taaagataat gaatatgaag
    40981 agattatact aaacatgatt gaaaaatcaa attgtacaga tgattcagag atagtattat
    41041 ctaaaaaaga taaaaaaaca ttaaaagatg tgctatcaaa aaaaggcatt aaagtgtctg
    41101 atgaaactag agatattact ggaggtttca tagttaaaaa aggtgacatt gagtacaatt
    41161 attcatttga agccataatt gcagtagagc atgaatatat tgaacaaatt gcagcagaaa
    41221 ttttgtttaa ttaaggaggg gttaaaaaat ggcagaagaa aaaacttatc cctatgccgt
    41281 tgcacgaata agagtgcttg aaaaacagct tcttaacaga caaatgttta ttcaaatggc
    41341 agaagccaaa agccctgaag actctttaag aattatagct gaggcaggtt atgctgatac
    41401 atcaaataat aatatacata attttgagaa tattttgact caagaattat caaaaacata
    41461 tgaaattctt aaaagtctag ctcctgaaga aaaatttgtt gatgtgtttc tttataaaaa
    41521 tgactatcac aatttaaaag tgctgattaa agaagaaatt tctggtgtag atggtgagaa
    41581 atatttaatt gatggaggta caattcctct tattaagctt agagaatctt tagcaaatcg
    41641 tagttttagt gatttaccta aggtaatgtc atcagctata ttagaggcat ttgatgttta
    41701 tagtaagaca caaaatggcc agatggttga tattgttcta gataaagcga cttttactag
    41761 tatgaaagaa actgctaaag aaagtcaaaa caaatttgta atagattatg ttatgaaagt
    41821 ttgtgattta acaaatctaa agagttttat aagagtcaaa aatatgaaga aagattttga
    41881 tatgtttatg aatgtgtttg tttcaggtgg ctcacttgat aaggaaaaat ttttagaagc
    41941 tttttcctca gatactccag catcatgttt taaatctact tcttactctg atgtttgtaa
    42001 aaatggcatg gatagtggtt ttactgtctt tgaaaaactt tgtgatgact atcttatgga
    42061 atatgttaga ggggcaaaat ttaagccact tacattagaa ccactaattg catatttata
    42121 tgctaaagaa tcagaaataa aaacaattag aattattttg actagtaagc ttaataatat
    42181 agacgcagat actataaaag aaaggctaag ggatgcttat gtataaggta ggagtagttg
    42241 gagataaaga tagcatcatg ggatttttag ctcttggaat agatatcttc ccagcctatg
    42301 atagtgacga aattaaaaaa agtatacata agttggttga agatgaatat gcgataattt
    42361 atattacaga acaggcatca ttgcttgcaa aagaatcaat agcaaaatac aaagattacc
    42421 aacttcctgc tattatagtt attccaggta ttggaggaag tatgggacta ggtatgaatg
    42481 aagtaagaga atctagtaaa cgtgctatag gtgcagatat attatttaaa gaatagatga
    42541 aagttgaata aaagacaatc tcaaatttta ttggtattaa agatatctac taataaaata
    42601 taggagggaa tatttaaatt ttgataaagt atatgattgc aggtgagaga atatgaaaac
    42661 aggcacaatt gtaaaggtgt caggaccgct tgttgtagcg gagggcatga gagatgcgaa
    42721 tatgtttgac gtcgtaagag tttcagataa acatcttata ggcgagatta tagaaatgca
    42781 tggagataaa gcttctatac aggtttatga ggagacttct ggtctaggac caggggaaga
    42841 agtagtttct cttggaatgc ctatgagtgt tgaacttgga ccaggtctta tttcaactat
    42901 ttatgatggt attcaacgtc cacttgaaaa aatgtatgat atttcaggta ctaatataac
    42961 taaaggggtt gaagtatctt cacttgatag aacaattaag tgggaattta aacctaagaa
    43021 atctgttggt gataaggttg tagcaggaga tatcattggt ggagttcaag aaacagcagt
    43081 agtagaatgt aaaattatgg ttccttatgg agttaaggga actataaaag aaatatattc
    43141 tggtgaattt actgttgaag atacagtttg tgttattaca gatgaaaaag gtaatgatat
    43201 acctgtaact atgatgcaaa aatggccagt tagaaaagaa agaccatata gagaaaagaa
    43261 aacaccagat tcacttcttg ttacaggtca aagagttata gatacatttt tcccaattac
    43321 aaaaggtggg gtagcagcta taccagggcc ttttggaagt ggaaaaactg ttacacagca
    43381 tcagcttgct aaatgggcag atgcagatat agttgtatat ataggctgtg gcgagcgtgg
    43441 aaatgaaatg acagatgttc ttcttgaatt tcctgaatta aaagacccaa gaacaggcga
    43501 gtcacttatg caaagaactg tgcttatagc aaatacatca gatatgccag ttgctgcacg
    43561 tgaagcttct atatacactg gtattacaat agctgaatat tttagagata tgggatatag
    43621 tgttgcactt atggcagact ctacatcaag atgggctgag gctcttagag agatgagtgg
    43681 tcgtctagag gagatgcctg gtgaagaagg ttatcctgca tacttaggtt cacgtcttgc
    43741 tcaattctat gagagagcag gaaaggtaaa ttgtctaggt atggatgaaa gagaaggaac
    43801 acttacagca attggtgcag tttctcctcc tggtggtgat acatcagaac ctgtcagcca
    43861 agcaacactt cgtatagtaa aagtattctg gggtcttgat gcatctttag catataaacg
    43921 tcatttccca gcaattaact ggttgactag ttattcactc tatcaggaaa aagttgatgt
    43981 atgggcagat aaaaatgtaa atgataattt ctcagctcaa agagctcaag ctatgaaact
    44041 tttacaagtt gaatctgaac ttcaagaaat agttcaattg gttggtgttg actcactttc
    44101 agatggagat agattgatac tagaggttac tcgttctata agagaggact tccttcatca
    44161 aaactctttc catgaagttg atacttatac atcacttcat aaacaatata gaatgatggg
    44221 acttatatta aaattctata agtcagcaca agatgctatc aagcaaaatg ttactataaa
    44281 cgatattatc aagctttcag ttcttgaaaa gattggtcgt gctaagtatg tagaggaagg
    44341 cgacatagat gcagcttacg attcaattga agatgaaata gacaacgagc ttgcagacct
    44401 tattaaaaag aggggggctg aagaactatg ataaaggaat ataagtcaat acaagaggtg
    44461 gccggtcctc ttatgctaat aaacggcgta gaaggtgtaa aatttgatga acttggagaa
    44521 attgaacttt caaatggaga gattcgccgt tgtaaagttc tagaggtaaa cggagatact
    44581 gcacttgttc agcttttcga aagctcaaca ggtataaacc ttgctgaaag taaagttcgt
    44641 ttcttaggaa agagtcttga ctttggagtt tcaccagata tattaggtcg tgtttttagt
    44701 ggaatgggta gacctattga tggaggtcca gaaattatac cagataaaag acttgatata
    44761 aatggagctc caataaatcc agcagcacgt gactatccgg ctgaatttat acaaactgga
    44821 gtttctgcca tagatggact taatacactg gttagagggc aaaaacttcc tatattctca
    44881 gggtctggtt tgccacatgc caatcttgca gttcagattg caagacaggc aaaagtacgt
    44941 ggaactgatt ctaagttcgc cgttgtattt gctgcagttg gtataacttt tgaagatgct
    45001 gaatttttta taagtgattt caaagcaact ggtgcaatcg accgttctgt agtgtttgta
    45061 aaccttgcaa atgacccagc tgtagagcgt gtatctacac ctagaatggc acttacagca
    45121 gcagaatacc ttgcttttga gcaagatatg catgtacttg ttataataac agatattaca
    45181 aactatgcag aggcattgcg tgaagtttca gcagctaaaa aagaagttcc aggacgtcgt
    45241 ggatatccag gatatcttta tactgacctt gctactatgt atgaaagagc aggaaaaatg
    45301 aaagggcatg aagggtctat aactatgatt ccaatactta caatgcctga ggatgataaa
    45361 acacatccaa ttcctgacct tacaggatac attacagaag gacaaattat acttagccgt
    45421 gaactttata aaaaagatat acagcctcct atagatgttt taccatcact ttctcgtcta
    45481 aaagataaag gtattggtaa gggtaagact cgtgaagacc atgcagatac aatgaaccag
    45541 ctttttgcag cttattcaag aggtaaggat gcaaaagagc ttatggctat acttggtgaa
    45601 tctgcacttt ctgaaattga tttaatgtat gctaagtttg cggatgaatt tgagaaagaa
    45661 tatgtttcac aaggatttag aactgataga actattgagc agacattaga aatcggatgg
    45721 aagcttctta gtatgttacc aaaaagtgaa cttaaacgta ttcgtgatga atattttgaa
    45781 aaatatatgc ctaaggagtg atatctgtgg ctagattaaa tataaatcca acacgtatgg
    45841 aaatgactag acttaagaaa ctccttaaaa ctgctacaag aggtcataag cttcttaaag
    45901 ataaacttga tgaacttatg aaacaatttc ttgagattgt aagagaaaat aagcgattac
    45961 gtgaagaagc agaaaatgct cttgatgctg cttataaaaa ttttataatc gcaagagcag
    46021 ttatgagtca ggagtatctg ggttcagcac ttatgatgcc aaagcaatct gtttcagtag
    46081 atgtttctac tagaaatata atgagtgttg atgttcctgt gtttgacttt aaaactgaaa
    46141 acaatcaaag tgatatttat ccttatggtc ttgcttttac atcaggtgaa ttagattctg
    46201 ctatggaagc tttctcagct gctatgcaac cacttttaag acttgcagaa agtgaaaaaa
    46261 gtgcacagct tcttgcacaa gagatagaaa agacacgtag acgtgttaat gctcttgaaa
    46321 atgtaatgat accaaattac attgagacta ttaagtatat agctatgaag cttgaagaaa
    46381 atgagagagc aagtactact agacttatga aagttaagga tatggtgctt aaaaaagcac
    46441 ttgaagaaaa gaaaaaaaat gatttggttg tataattaaa tacttataaa aataagtcta
    46501 acattctaat ttatagaggt taggcttatt tttatgttta aattaaaagt gtaaatagta
    46561 taaaaatcaa tacattgttt aaaaacagac tgtatttaac taaaaataat atatttaaca
    46621 aaaatttcta aaaattaacg aactgttaat ttacacttaa ctttatatta acaaattagg
    46681 tgtattatat aaacataagc aaaaacaata aaaaacaaat aaaaaaagaa aaaaacacaa
    46741 agggggaaga tatgaaagaa tttatattta cttgtctgtg cattgtagtg attacattta
    46801 tgacaagttg catatcaaca acaccacaaa ctactcaagg aaaaggaaat aataaactta
    46861 caatatatgc agcactagag gaagagcaga tagcttacta tatggaaggt tttagagaag
    46921 agtatcccaa tatagatgta gagatagtta tggattctca tggtgtaata gcttcaaaat
    46981 tattagcaga aaaagataat ccacaagctg atgttatatg gggattatca gcaatgaaca
    47041 tgatagaact aaaagaagat ggtgcacttg aagcatataa acctaaacac tatgataaag
    47101 ttgataaaaa attcaaagat actgaagatg atgtatcttg ggtaggaatg agtgtcccgg
    47161 aaacagcaat agtagttaat tataaggaac ttaagaaact aggtatagat attccaaaaa
    47221 gttatgaaga tttaataaaa ccagaatata aaggacttat aacaatgcca aatccagcat
    47281 catctggaac agggtatctt acagtatcag gccttattca aatgatgggt gcaaaagatg
    47341 cttacaatta catggataaa ttacataaaa atatagctac atatacacac tcaggttcaa
    47401 aacctgcgaa actagctggt tctggagagt atccaatagg tattacactt ggatatagag
    47461 ctacacaaca attatccagt ggagaacctg tagaagttgt atttccaaag gaaggttcag
    47521 gttgggactt agaagcaaat gccttagtta aaaaagataa tataaaaaaa gaagcaaagt
    47581 tatttttaga ttgggcaata agtgattcag ctatggatag atattcacat gaagtagcag
    47641 ttacttccat aaagacagat aatccagttc caaaaggtta ctctaaacaa ccatttgaac
    47701 agcttataaa agaagttgat ttaagttctg cggctaaaaa cagaagtaca gtacttaaaa
    47761 cttgggaatc taaatatggt tcaaaatctg aaagtaagta aagtttaaat taataattta
    47821 gatgaaagaa ttttagaaaa aaaggtagga tatagaattt aagataaaaa tataactata
    47881 taaggagaga taagatatga gttatttaaa aataaagaat gtgtttaaaa attatgacca
    47941 aaaaagagtt ttaaataata ttagtttgga tattgaagaa ggggaatttc tatgtttact
    48001 tgggcctagt ggatgtggta aaactacatt acttagaata atagcaggtc ttgaagatgt
    48061 aaatagtgga gatataattc ttcaggataa agatattaca aatttagaac catcaaagag
    48121 aggttttggt atagtatttc aatcatatgc attatttccg aatatgactg cttataacaa
    48181 tatagctttt ccactaaaag agagaaaaat ttcaaaagaa aaaatagata ataaagttaa
    48241 ggaagtactt gaaatagttg ggttgaccaa tgaagctcat aaatatccta aagcattatc
    48301 aggtggacaa caacagagga ttgcaatagc aagagcatta gcacttgagc cgaagttttt
    48361 acttttagat gaaccaatgt ctgcattaga tgcaaaggtt agacacaagc ttagaatgga
    48421 cattaaaagg ctacaaaaag aacttaatat aacgacaata atggtaacac atgaccaaga
    48481 agaagctata actatggcag acaaaatagc tattctaaat ggaggagata taatgcaaat
    48541 aggaactcca gaggaaatat accaaaatcc tcaaaaccta tttacagctc aatttatagg
    48601 agatacaaac tgttttgata atggagattc tatattaact gtaagaccag aatatgttca
    48661 aatagagaaa tcgacaaaag aaaattacca aggtataata tcaaatatag agtttagagg
    48721 gaatttatta agagttgaaa taaaagataa actaaatgaa aatttcataa taagtgatgt
    48781 ttctataaaa gagtgggtta atttaaattt agtagaaggt gaccttgtaa agataagtat
    48841 agacgaaaaa tactatttaa agtatccaaa agttaaagga gcataaaggt atgaataata
    48901 atgctatagg ttataaaagt ataagtctta aatcaaaaat gaatttagat ataaaaaaat
    48961 gtatgcttac agtattagtt atgtctttag taatattttt attggcacct ataatatatc
    49021 tatttgtaca ggctttttta gataaagatg gagtctttgt aggattacaa aactttcaag
    49081 aatacttggc ttctgaaact ctattaaatt ctctaaaaaa tacaatattt gtttcagtta
    49141 tatcaactgt aatatcacta gcactagcac tagtatatgc atatgcatta actagaacaa
    49201 atcttaaatt taaaggtttt tttaaatcat tagcaatgtt tcctatgttt gctccaacaa
    49261 tgatgcatgc aataggtcta gtatatttat ttgggaatca aggattggta actaatttac
    49321 ttggaataca tgttccaatt tacggagcag taggaataat actggcacag attatatatg
    49381 tattcccaca atcatttcaa atactttata tatctctaaa ttcaactgat tatagactat
    49441 atgaagctag taatactatg gggataagca agataagaga atttataagc ataacaattc
    49501 caagtataaa atactcttta ataagttctg cattcgtatc gtttacatta tgctttacag
    49561 attttggagc tccaaaggta ataggaggag agtttaatgt attatcaata gatgtttata
    49621 agcaaattat agggcaacag aatatgccaa tgggagcaac tgtatcaata ttacttttaa
    49681 tacctgcaat aatatcgttt atagtaacta aaaaaattga aaaaaatcaa aactcatata
    49741 taacttcaaa gtcaattcca tataaggtta aaactaataa gttaagagat agtattttag
    49801 gaggaattgt aggaattatt gcatttggta taatatctgt aatgttaact gtaatcttat
    49861 catcattaat atcagtttgg ccatatgatt taaggcttac actggaacac ttttctatga
    49921 aaaatagtat tgatggaatt aaaccattta taaattcaat aaaaatgtca ttattgacag
    49981 ccattctagg gacgagcttt gttttcttat ttgcatattt aaatgaaaaa acagaaaaag
    50041 ccccctattt aaaatcattt gggtattttt taagtacatt gcctatggca ttgccaggat
    50101 tagttttagg tatagcttat gtattattct ttaataaatt ggagtttgat tttatggata
    50161 caatatacct taaaaatctg tttaatggat tatatggaac attgcttatt atggttatat
    50221 gtaatatagt tcatttcttt tcagtagcat ttataacagc tactactgct attaagaaag
    50281 tagataaaga attggatatg gtagctaaat caatgggaat aaacagcgca tcaattctta
    50341 agaatgtaac tataccactt tgtttgccag cagttttaga gaactttatg tactttttcc
    50401 taaactctat ggttacaata tcagcactag tttttatata tactgcaagt tcaaaagtag
    50461 ctgcaatatc aattgtacaa ctagatgata aggggagtac tgcacaggca gcagcactag
    50521 ctgtgttaat attaatcaca aatatattag tgaaagcaat acatgaattt ataaagaatc
    50581 gtttagaaaa aagaacaaac tttaattaaa gaaattattc tacaaaatta taaatttaaa
    50641 aagtctattc tcacaattta ttatttacag aatagacttt ttaaatttat tattaatatt
    50701 atttattaat tttccttttg ttatcataca tttgagaatc tgcataatta tacttataaa
    50761 ataaagaaat atttattaaa attttctgta aaatttttag ttttactgga agattagatt
    50821 cagaatatta tcaagctaag tttgactaat tattaagttt cgcatcaaaa caatgtttta
    50881 atagtgttta cagttgaagt tttttataaa aagtttagtt cccgtttaaa atttatattt
    50941 attttgatat tttcttaatt tataatatat tatattaata catagttatt agaaataaga
    51001 cacaattaaa taattatata aaattgggag gttaaaatgg gaagggccaa atattatgat
    51061 atatatatag atttaaaaaa tagaatagaa aacaaggaat atttatacca agatttatta
    51121 ccatcagaat acacattaat agaagagtat aattgttcta gaaatacaat tagaagagct
    51181 atacaggaat tagcaaacca aggatatgtt caaagtaagc atggaaaagg agtatttata
    51241 atttacaagg ctaacaagca atcagagttt ttattaggag gaacagaatc atttaaagaa
    51301 tctgctatta gaaacaagaa agaatataaa acgaaagtag ttcattttga ggaaatggtt
    51361 atagatgata agaaaagtca aataactaac tttgaagttg ggacagaagt gtattatata
    51421 caaagagtaa gaattctaga aaataaacca tctattatag atcataatta ttttatgaaa
    51481 gatatagtaa aagatattaa tgaagaaata gcatctaatt ctatatatga atatatggaa
    51541 agtgaattaa atgagactat agtaacaaca aaaagaacta tgacagttga aaaagcaact
    51601 aaactagata aaaagtattt agaattagga gattataatt gtgttgcagt tgttagcaat
    51661 aatacttata atgcccaagg tataatgttt gaatatacgc agtctagaca catacctgat
    51721 ggattcatat tttttcatca atctcaaaga attaataata taaaatagag tacaatttgt
    51781 actctatttt atattattaa ttctttgaga gttatactca taaactttag cagctaagta
    51841 tatgaatgta ctagctgtcc aggtgaaaga tttatctcta agaccctctc ctgtaatagc
    51901 gttgaaattt tcagcaaagc cgtttttatc aactaattca caatatcttt tggcaatatc
    51961 tattgcaagt tcattttcac cacattcttc taaagcttca acaaataata gtgtagtagg
    52021 agcccaaata ggccctctcc aataagcatc atcttgatat tcatcactat ctatagcttc
    52081 tgttgcgatt ccccatttag ttaaatatct ttcgctagtg actaaatcta tcattttgtc
    52141 tctaatatat ttaggaagtc tatttcctaa aactaaagaa acaagaggaa gtaaagcgtt
    52201 gttagaaagt atttcaccag ttacagtttc gcgagctaca ggtaaatcat caactataaa
    52261 ataatttaag aataactttg taagttcatc tgctttatta gaccaatact ctttttcact
    52321 atttctgttt agtttttcag ctacttttga tagcatgtcc atagcattta ttataaatgc
    52381 tgttaaatca ggagtatcaa caaccatagc tcctttaaat attgtactat tatcctgtcc
    52441 agaatcattt ccatggtgat attcaaaaat accatccata tttgaatctt taaactttac
    52501 ataaaacaat acttgttttt ctatgtaatt ataaattttt tctaatgttt ctttagtaaa
    52561 gttaattttt ttcatcatct tactaaaaaa taatccttga ataggtggtt ttgtaaaatt
    52621 ccatctaata gtagaatcgc ttaaggaccc aggaatttga ccatattcat cttggtagtc
    52681 gaaaagtact gccatttggt caaaagctaa tttatcattt acaccagcta aagctaatgc
    52741 gttaaaacag tgatcccagc tccatgcacc taagaatgtt gagtttgaag caagtatagt
    52801 atatctcttt aggtatcctt gaggtttgac aaaagaagac caatttatat aactcgcttt
    52861 taaaagtgag tttttatatt tttcatctac tttaggttga gctagataat attcatcaaa
    52921 taattttttt gtgctattta gtgaagtttc aaagttatat tccttgtaat ctggaacttt
    52981 catatgagta ggaatgtctt ctataacaca tataaattca ttatcactat tacttttaac
    53041 atctattatt gaagtattat tagctttatt tgtagaagca cagtcatcta taaatatatt
    53101 ttgtgataag ccaaccttac cattaacaga gaatactaaa aacttagtta aatttttgta
    53161 gctatttata acacaatatt ctgaaccttt tttacccata aggtagttat attcaaaatt
    53221 gtatatggga agtgtatcta gtttaagacc tacatttttt cccttaccat agaatacgat
    53281 tttagaatct ccttcaaaac atatagatat actaccattg ttgaatttaa ttaatatact
    53341 aaaatagtta gcctcaaatt cataatctac aggcatgtta tttactgtag gtataatcct
    53401 tagtacatcc atatttttct ttgattttcc tcttaatgat tttaatgtaa atccaagttc
    53461 atctgaataa gaaatagaca tataagatcc aaagtaacta aacggaattt cttttatatt
    53521 catttggtat agtcctttca tgatttattt tatagattta ctctgcttgt ttatacaagt
    53581 aagtatatat ttaacttacc aaataataaa ataattatca agagttaatt tatcaaaaaa
    53641 ataaataaga aaatgttttc aaaaaactat tgactaaaat aatataaaat attacaataa
    53701 agttgttcaa acaagttact tgtttaaaca acttattact tataataaaa aagagaaagg
    53761 ataagtagat atgaggaaga ataagatact aaattcaaaa gaaagtattt ccaaaattat
    53821 tgagtatgtt gggggaaaag aaaatataca aagtgcaaca cactgtgtaa caagattacg
    53881 tttaatatta aaagatacta gcaaagcaaa tattagtaaa ctagaagaca ttgatatagt
    53941 aaaggggtgt ttcaatgcag gcggacagtt tcaaatagta ataggaactg gagtagataa
    54001 agtatataaa gatttaatag accaaacagg agcagaagaa gtatccaaag aagaaattaa
    54061 acaacaagtc aatgaaaagt caaataaatt tcaaaagttt ataagaatcc ttgcagatat
    54121 attcattcct atattaccag ctatagttgc atcagggtta cttatgggtg ttaataatat
    54181 gatagctaat ccagggattt tttatgagaa gtctgttata gaagtaaaca cagatatagc
    54241 tgggatttca ggtattataa atttaatagc aaacacatca tttacatttt taccagcatt
    54301 aataggttgg tcagctgcga agaaatttgg aggaaatcct ttattaggtg tagttttagg
    54361 tcttgtatta gttcacccag ctttgatgtc tgcatatgat tttgcagcaa atccatcagc
    54421 agcaccaaca tgggatatat ttggtatgaa ggttgctcaa gttggatacc aaggtcaagt
    54481 aataccagtt ttaatagcta catatatttt agctaaactt gaattattct taaagaaaac
    54541 tgttccagat gcagttcaac tagtattagt ttcaccaata gctttattaa ttacaggtat
    54601 gttaactttt gtagttatag gacctataac tatgacaggt gcaaatatga taactaatgg
    54661 agtagtatat ttatttgaag tagcaccagt tttcgcagga gcactatatt catttatttc
    54721 accaccatta gtaataacag gaatgcatca tttgttttta ggagtaaact tacaaatggc
    54781 aggaacttta ggatatgtaa cattgtggcc aatatcagag actgttactt tagctcaagg
    54841 tgctgccaca ttggcaatgt ttttcatact taaaaataat aagaatttaa ggggtgtatc
    54901 tattacatct actatatcag catggctagg aataacagaa ccagctatat atggtataaa
    54961 cttaagattt agatatccat ttatagcagt tatggtagca agtgcaatag gaggagcatt
    55021 tatatcatat cacaatgtta aagctacagc tgttggggtt ggcggagtat tttcattcct
    55081 ttcagtgatg cctcaatatt ggggagtata ttttacggga atggcaataa catttgtaat
    55141 aacaatagga ttaacatact tattctataa gagtggtaaa tttaaaacaa atgacaatat
    55201 ataaatataa ttcccttggt tttatatcaa gggatgttaa tatgcattgg atagatagga
    55261 gacaatatgt tagattttaa aaagagtata gtgtatcaaa tatatttaaa gtcgtttaat
    55321 gatacaacaa ataatggttt aggagattta aatggaataa cggaaaaatt agattattta
    55381 gaatttttag gtgttgatta tgtgtggatt actccatttt ttgtatcacc acaaaatgat
    55441 aatggatatg atgtagctga ttattataat atagacccta tatatggaac tatggatgat
    55501 ttagaaaatt taataaaaga agctaaaaaa agaaatatag gtatcatgct tgacatggtg
    55561 tttaatcata cgtctataga gaatgagtgg ttcaaaaaag cacttaaagg agatgaatac
    55621 tacaaagatt tttatatatt taaaaatcct aaagaagata aaagtgctcc aacaaattgg
    55681 gaatcaaaat ttggaggcaa tgcttgggaa tttgtagaag aatttaatca atattattta
    55741 catttatttg ataaaactca agcagattta aactgggaaa atcctaatgt aagaaaagaa
    55801 ttaaaaaata tattaaagtt ttggatagat aaaggtataa aaggatttag atttgatgtc
    55861 gtaaatttaa tatctaaacc agagaagtat gaagatgatt tacaagatga tggtagacgt
    55921 ttttacacag atggtaaaaa agtacatcaa tacttaaaag agatgtgtga agaaagtgga
    55981 atattagaaa atgatatgtt aactgtagga gaaatgtctt ctacgactat agaaaactgt
    56041 gtaaaatatt caaatccaga tgagaaagaa ttgtctatgt gttttaattt tcatcattta
    56101 aaagttgatt ataaaaatca aaataaatgg atgttgcaag attgtgattt tattaagtta
    56161 aaaaaaatac ttaatgagtg gcaaatagga atgcaaaaag gtaatggatg gaatgcttta
    56221 ttttggtgta accatgacca accacgcata atatctagat ttggagatga taaaaagtat
    56281 tataaagaat cggctaaaat gttagctact atgatttatc tcttaagagg aacaccatat
    56341 atatatcaag gtgaagaaat aggtatgact aatgcatgtt ttactaatat aaaacaatat
    56401 gtagatgttg agagtttaaa ttattttgat atattaaaat ctaaaggaat gaaagatggt
    56461 gagatataca atatattaca agaaaggtct cgtgataatt caagaacacc tatgcaatgg
    56521 aataatagta agaatgcagg ttttagcgaa tcaaaatcat ggatagaggt tataaataat
    56581 tatagaaata taaatgtaga aaatagttta aaagataagg attctatttt ttattattat
    56641 aagaagttaa tagcattaag aaaaaagtat gatgtagtag cctatgggga ttttagacca
    56701 ttattagaag aacatgattc tatatttgca tataagagat gttacgaaaa agaatcttta
    56761 gtagtgttaa ataactttta tgataaagaa attgatgttg ttttagagaa tgaagagctt
    56821 gaaaaataca agtgtataat atctaattat ggggacagaa aaattgtttc aacaattact
    56881 ttaagaccgt atgagtgtat agctttattt aaaaatgaaa aataatctaa aattaaaatc
    56941 atgaaaaatt tatgtatact ttttagataa attttatttt tgaagtcata atttaatatt
    57001 tattagtaag gctgtgattg taaaaatcat agccttattt taatattttt tccacatata
    57061 caaattattg tttatattaa ggaaatttaa tttatattaa atgtatagac attttctttg
    57121 tttgtatata aaattatctt attatataaa aaatttaatt aatgaggagt gaattaaatg
    57181 aaagagccaa tttataaagt tatagaaaat catgttagag agttaataaa ttcagacagc
    57241 ttgaaagaag gggatttaat cccttctgaa aaacagttga gtgaagagtt taacgtaacg
    57301 aggatgactg taaggtctgc acttaataac ctagttaaag aaggatatat aactagacaa
    57361 agaggagtag gtagtatagt tcttgcaaat aatatatacg ataatatatc atctgtaagt
    57421 gggtttacta aagagatgga aagcaaaggg tataaagtat ctaatatatt ggtatctctt
    57481 gaaatagttc aagcagatga agaactatca ggtaaattaa atataagttt agaagaaaat
    57541 gtttgggaaa tcaaaagggt aaggctagca aatgatgcaa gagtttcata tatgattact
    57601 tatatgccag ttaagttatt tccaaattta aataagactc attgtgaaaa ttctctttat
    57661 aattttgttg aagaagtgtg tgcatataag atagccatgt cagagagaga agtacaggca
    57721 gtaatatcga ataaagagtg tatggataat ctaaaactag aagaaccaga gccactttta
    57781 tatataagcc aaatctgtaa gttacaaaat agtgagattt ttgaatactc acacacatat
    57841 cactatggat atacattaac tttgaatgct gttgtcgaat aaaatttaaa taaaatgaaa
    57901 aagttttctt gaaatgtata tacatttggt atataataat atcaagaatt aaatgtatat
    57961 acatttaaaa ataagatata tacattaaga aaagattaat agaaaagcat actttaaatg
    58021 tatagtttaa aaaacttatg acttaaaaag tatttaatga atttaatttg gaggggaaaa
    58081 aatgataaaa ataatgttag cttgttcagc aggaatgtca actagtttat tggttacaaa
    58141 aatggaggat gcagcaaagg aaaacggtat agaagcaaaa atatgggcaa tatcagaagt
    58201 gaacttaaaa aatgaaatag aaaattgtga tgtcttactt ttaggacctc aagttagata
    58261 tgtattggga aaagcaactg aaatggcaaa accatataat attccagtag aagttataaa
    58321 tatgatggat tatggaagat gtaatggaaa agctgtgtta gacagagctg tagagttgaa
    58381 tagttcaaaa taaagtcgta ctggaggatg taaaatggaa aaattcatgt catttatgga
    58441 taaatatata gtgcctgtag ctgctaaaat aggagctcaa aggcacttgg tagcagtaag
    58501 ggatgcattt atagtaatga tacctataac tatggtaggt gcattaggaa cattgataaa
    58561 taacttacca ttagaagcat acaaaaattt gatggcaagt atatttggag aaaactggac
    58621 tacgtttgga ggagaccttt ggtggggagc aataggtaca atggcagtct tcttagtaat
    58681 aggtgttgcc tattttctag caaaatctta tgaaagtgat ggattacaag ctgggcttat
    58741 agcattatct atctttttta taatggcccc acaaattggt aaaatagttc ctgagggagg
    58801 aactacagta gttgaaggat ggggaatgat acaacaaact tacttaggta cagcagcctt
    58861 attctcttct atattaatag gattgctatc aactgagata tttgtaagat tatctaaagt
    58921 aaagaaatta actataaaaa tgccagatgg agttccacca gcagtttcaa gatcatttgc
    58981 aaagttaata ccaggaatgt taactataat gatatttact gtaataggaa tatttataaa
    59041 aatgctttca aatggaagtt tcttaacaga tattttaaat acttatttag gagcaccact
    59101 atcaaatgta gctgatagcc taggttctac aatgcttatt gcatttataa ttcatatact
    59161 ttggactgtt ggacttcatg gtgcaaatat agcacttcct tttacagaaa ctatacttat
    59221 gaaattaggt ggagaaaatg ctgccttggc tcaagcagga gcaacagaag gataccatgt
    59281 acttgcaggt gcattctttg atgcatttgt atatttaggc ggttctggaa tggtattggg
    59341 attaattgta gctttattaa tagctggaag aagacgtaaa gaaatgatag tattaggtgg
    59401 accacctgca atatttaaca taggagaacc attgatattc ggacttccaa tagttttaaa
    59461 tcctatattt atgattccat ttgtacttgc accagtaata tgtagtgctg tatcatactt
    59521 agcaatagat tttgggttag tagcaccagt aattttacct aaaatacctt gggtaacacc
    59581 tcctatatta ggtggagcaa tggctacagg tgactggact ggtggagctt tagcactatt
    59641 taatttaata ttatcaatat taatatatat tccatttgtt atagcgtctg aaaaaatgga
    59701 ggctaataaa ttgaaaatta acaactaggt agttttattt cggtaaataa atgtataggg
    59761 ttttgtatag tgtattaaat tataggtaag gatggtataa tgagatgaaa aagggactaa
    59821 aaatagttac aatcggagga ggttcaagtt atactcctga gcttgtagag ggttttataa
    59881 atagatatga agagctacct gtaaaggaat tatggcttgt agatatagaa gatggaaaag
    59941 aaaaactaga gatagttggg aatctagcta aaaggatggt aaaaaaagct ggaatagata
    60001 tgcaaataaa tcttacctta gatagaagag aagcactaaa agatgcagat tttgtgacaa
    60061 cacaacttag agtaggtctt ttagatgcga gaataaaaga tgaaactatt cctttatctc
    60121 atggagttat gggacaggaa actaatggtg ctggaggttt atttaaagcc cttcgcacaa
    60181 taccagttat atttgatata ataaaagatg tggaagaatt gtgtccaaat gcatggatgg
    60241 tgaattttac aaatccaaca ggaataataa cagaggctgt attcaaatat actaatttta
    60301 gaagatatat aggtctatgc aatgtgccaa ttcatttaaa aaatgatgtt gccaaactat
    60361 ttaatgtaga aggtgacaga ataagtatgg attttgcagg tcttaatcat atggtttatg
    60421 gtctaaatgt atctttagat ggagaagatg ttacaaaaga agcaattgat aagtttgtca
    60481 aagcagatat aagtatgcaa aatataaagg caattgattt taatgctgaa tttgtaaaat
    60541 ctttgggtgc gataccttgt ccatatcata gatactacta taaaactaaa gaaatgcttg
    60601 aagatgagtt aagagagttt aaagagggaa aagctagagg tcaagtagtt aaagaattgg
    60661 aagaacaatt atttgaatta tataaggatg aaaaattaga tgtaaaacca cctcaacttg
    60721 aaaaaagggg aggagcttac tatagtgatg ctgcttgtaa tttaataagt tctatataca
    60781 atgataaaaa agatatacaa gtagttaata cattaaataa tggttctata agagatttta
    60841 aagatgaaca agcagtagaa gtaagcagtg taataacaaa aaatggtcca aaaccaataa
    60901 gtatagggta tttaccagat tctgtgcatg gattagtaag ccaaatcaaa tcatttgaag
    60961 tattggcagc taaagcagcc gtatatggtg attatgaatc tgctcttttg gcactttgta
    61021 taaacccatt gattccatct gatgacttag caaaaactat attagacgaa atgttggaag
    61081 ctcataaaga ttatcttcct aaatttaata gatagttgtc tatagaaagt aggttatggt
    61141 atgactaaaa ttataataaa tgcagatgac tttggatatt gtgaagctgt gaactatggg
    61201 atagtgtcag ttcataataa tggtatagtt aggtctactt ctatgatggc aaatatgcct
    61261 ggggtagaac atggagtagg gttacttaaa gaaaataagt ctttaaattg tggagttcat
    61321 atgactttaa gttgtggtag accattattg tctaacttaa aaactattgt tgataaagac
    61381 ggttttttta ttaggaggat tacagacgaa attattgaaa aaatggatta tgatgagatt
    61441 tatagagaac tttgtgctca aatagataga gtaaaagggc ttggaataga tatatctcat
    61501 ttagacagtc accatcatat ccatacttta ataagtttaa agccagtaat agagaagatt
    61561 gtgaacaagt ataatcttcc aataagaggt ggatttgaat ataatttaga gtattctaaa
    61621 atagttcctt taatagatag tttttataag gaaaatgtat gtgaagaata ttttataaaa
    61681 aatatagacg aaataatgaa gtatgatgta gtggatatta tgtctcaccc agctttttta
    61741 gatgattata ttttaaattc tacatcttat gctattgata gaacgaaaga gcacaagata
    61801 cttacatcta aaaaagtcaa agaattttta gataagaatg atttagtcat atcaagttat
    61861 agagatattt aggaggacga ataaatggat gagaagatga tagagatatc ttttggaata
    61921 attggatttg caggtgatgc gaaagggcta gcatttgaag caataaaaga agcaaagagc
    61981 gggaatatag ataaagctag agaatgtatt gaaaagtcga aagaatcaat tcataaagct
    62041 cataaattcc aaacagaatt gatacaaaat gaagctaatg gtaataaaac tgaaataagt
    62101 gtaatactta ttcatgcaca agaccattta atgacagcta tgaattttca acaattagca
    62161 gaagaattta tagatgtata tgaaagatta gaaagtaagt aatttaaaat tattattaaa
    62221 aaagagtgcc tttaataatt ataaaaatta tttttataat tatta
//
LOCUS       DAFNNB010000005        54778 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0005, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000005 DAFNNB010000000
VERSION     DAFNNB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 54778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54778
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0005"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1644
                     /gene="cas3"
                     /locus_tag="L3M60_000273"
     CDS             <1..1644
                     /gene="cas3"
                     /locus_tag="L3M60_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HBN6224980.1"
                     /translation="TDEEIDNMILDEIYKSQGNLEKNNIEFNTIQKRLRELSGRNVLT
                     VAFTGSGKTAADYRKTFKRKFFLVPNKISAESFYRKNIFQNKNDLNTRNSNDYIGLLH
                     GDINLYSENEDNGEHDFVLTLRDIDLSINFCKPCVIATVDQLLLSMFKFPGYEKIFAA
                     VKNASITVDEVHLLEPKMFLILIYFMQFACKYLDVDFHLMTATLPKSYKEQMINKGII
                     FQEESNENVTDKGEIAFIESNKEENICEGKDVKVSFIKEKEIKSIVEGALENKQKILI
                     IKNTIDSVNRTYEFLKENLSDKYSGVDIDVLHSRFKFKDKKEKYSKILNGKGDIWIST
                     QSVEISLDLDFNIIISDLATMDSLIQRMGRCNRNNKYEYGNFYILPSEDKIYDNKLKN
                     TTKSILNNVIKTNSIFTMGIRKTILDDYYDNSVVKKYFEDNFISCDKEIKNIYGINKE
                     IFDGLDLIFNFEPYKNIVDSKKEAAKIFRDVDVSYKIILEEDFYKEDRELQQDSIQVS
                     GFIFNRLYYYRLISKVEGYMVLKSSSKFEYNSTVGLILK"
     gene            2083..3030
                     /gene="prmA"
                     /locus_tag="L3M60_000274"
     CDS             2083..3030
                     /gene="prmA"
                     /locus_tag="L3M60_000274"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBN6224981.1"
                     /translation="MNNWIEVTIKTTTEAVEPITNILYEQGAGGAVIEDPKDFLFQKK
                     NELDWDYVEEEVFKKNEEDDVLIKTYVSEEKNVMEFVEIIKQKVLGLKDFGIDIGEGS
                     VSLDQVNEADWANAWKAYYKPTKVGQRVVVKPTWEDYAMQDGDLIIELDPGMAFGTGT
                     HETTSMCIRELEKYVNKDSKVFDIGCGSGILAIAAAKLGAKEVVAVDLDEVAVKVAKE
                     NVLENKVEKSVSVMHGNLTDVIKDKADVIVANIIADIIKILAKDVQNFMKEDAIFISS
                     GIILDKVEEVKESLIENGFEIVEVQKLGEWSAIVSKLKN"
     gene            3049..3807
                     /locus_tag="L3M60_000275"
     CDS             3049..3807
                     /locus_tag="L3M60_000275"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBN6224982.1"
                     /translation="MDRFFVEKNNINLQDKTCTIEGEDVKHISKVLRYKPGEKLEICD
                     KNNNEYICEIVNIDKSIVNLNILEKVDINRESELKIRLYQGLPKAPKMEMILQKLTEV
                     GVEEIILVQTKRSVAKVDDKKEDKKFERWERIIYEAAKQSKRGKIPKLRGVLTFKEAL
                     EDMKKNNINICPYENERTVPMKHALQNCDSNIDSVGIFIGPEGGFSEEEIEQIQKNNC
                     NVVSLGPRILRTETASVVASTIALYELSDLGGEK"
     gene            3809..5107
                     /gene="mtaB"
                     /locus_tag="L3M60_000276"
     CDS             3809..5107
                     /gene="mtaB"
                     /locus_tag="L3M60_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HBN6224983.1"
                     /translation="MKKVAFYTLGCKVNQYETEAMLELFEKDGYEQVNSEEYADVYVI
                     NTCTVTHMSDRKSRQYIRRVKKKNPDAIIAVVGCYSQVSPEEILDIEEVNLVMGTNDR
                     RKIVEEIKKINSSKKVSTVDDIMKVKAFEEIEISQTNGKTRAFMKIQDGCDRFCTYCI
                     IPYARGRVRSRDIDSIVDEVKKLANNGYKEVVLTGIHVASYGKDLKDRDIKLLDVIKQ
                     INKIEKIERIRLSSVEPILFTDEFVNEVLKMDKVCPHYHLSLQSGCDETLKRMNRRYT
                     TLEYKTIVDRLRSKMPDVAITTDVIVGFPGETNEEFKKTYEFLKEIELSQMHIFKYSP
                     RKGTPAATMENQVDPQMKHFRSEQLLNLSKVNFNKFATKFIGRKLDVLFEQNLEGNKY
                     EGLTSNYIRVVVESDKNIQGQILKVKINDVKDEYVEGILL"
     gene            5150..5500
                     /locus_tag="L3M60_000277"
     CDS             5150..5500
                     /locus_tag="L3M60_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HBN6224984.1"
                     /translation="MDCIFCKIANGEIPSTKVYEDDRVLAFNDLNPVAPYHILVVPKK
                     HYDSLIDITDKEMDIVSHIHVVINKIAKEKGFDQTGFRVINNCGSDGGQEVKHLHYHI
                     LAGKKLPNYEAGQN"
     gene            5663..5842
                     /locus_tag="L3M60_000278"
     CDS             5663..5842
                     /locus_tag="L3M60_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBN6224985.1"
                     /translation="MSEVRVRENETLDSALRRFKRQCAMSGIMSEVRKREHYDKPSVK
                     RKKKAEAARRKNAKK"
     gene            5872..6315
                     /locus_tag="L3M60_000279"
     CDS             5872..6315
                     /locus_tag="L3M60_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HBN6224986.1"
                     /translation="MSLKQKLQEDLKSSMKNKDTVRKSVVTLIRASIKQYEVDNRVEL
                     DEDGIIDVIAKQLKQRRDALVEFEKAGREDLIKETEGEIEVLKEYLPQQLSEEELEEI
                     VKSTISEVGATSMKDMGKIMSVIQPKVKGRADGKLINKLVKQNLQ"
     gene            6426..6923
                     /locus_tag="L3M60_000280"
     CDS             6426..6923
                     /locus_tag="L3M60_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HBN6224987.1"
                     /translation="MIISVKEIRNFLINIFIPLIIGYLSSILTMIISGTDISTYYLQL
                     TKPSFAPPAFIFPIVWTILYILMGISSYLILRKGYNLPKVRDAIFYYGLQLVLNFIWS
                     ILFFGFGLRFTALIDIIIMILVSIIMISKFSKIDKRSGYINLVYLMWLVYAGFLNYFI
                     WFINK"
     gene            7116..7673
                     /gene="raiA"
                     /locus_tag="L3M60_000281"
     CDS             7116..7673
                     /gene="raiA"
                     /locus_tag="L3M60_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBN6224988.1"
                     /translation="MQIIVSGRQMKLTDGIKGYVDGKLSRLEKYLDPESEVKVTVSAK
                     KDRQKVEVTIIPINGQIIRAEDVEDDLYAAIDIVCDKLSRQVVKYKTKVKDKVQNNKS
                     IRFENVDFIDNSSEFDDYDDEEYENIVIERRKKFNVKPMSSEEAILQMELVGHNFYMF
                     RNQDNFEINIVYKRKAGGYGLIEQD"
     gene            7887..8123
                     /locus_tag="L3M60_000282"
     CDS             7887..8123
                     /locus_tag="L3M60_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YabP/YqfC family sporulation protein"
                     /protein_id="HBN6224989.1"
                     /translation="MLEISTDLQVNQPVITVTSNTFISIENYLSILEYEVDLIRIKTK
                     VKTIKISGDKLSLKYITDSEIGIKGIIYNVEYVD"
     gene            8136..9461
                     /gene="yqfD"
                     /locus_tag="L3M60_000283"
     CDS             8136..9461
                     /gene="yqfD"
                     /locus_tag="L3M60_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HBN6224990.1"
                     /translation="MISFIRGYYVIVVEGVGLEQFLNHLIRNGISVYNVTRIKNTKME
                     FHIDRQDIKEFKNVYRGSKFDIKVKQKTGVPFIIKRVYKHKGMWICALVSLFLLMSTS
                     QFVTDVYIQSPEGIKKEALRSELYKVGVRPGVYKKSIDRKEVRDHMMSKFNDVAYLSI
                     NVKGTNIFVTVTKKAESLKSTDQSNYCNVIALKNGIIEKVIPRSGKSVIKSGDIVQKG
                     DVLLNGANTKSIPEVWASTFYESTKKASYVDTVNKKTGEKKNIYTLSFYDKEFTMRKN
                     IKYKDYVVENKEKKLSIGNYTFPIKIKTSTFYETKKVEVKRNKEELKKELSEKALKEL
                     EYIIPASARIIDVKHNFKVNKNMLEYLITVQTSENIAKIYPLSKSEAERFIKEESKPD
                     EGEEEIPSNPEKRPLNDIRNEFDEDNKDNKEQNNSDENNSNQNSNSNNN"
     gene            9499..10515
                     /locus_tag="L3M60_000284"
     CDS             9499..10515
                     /locus_tag="L3M60_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBN6224991.1"
                     /translation="MIIQKKFTIADGNFERELFGNFDENVKLIEKTLNIDVILREGNI
                     ILIGEEKNVDSALKLMNELHQTVSNGKHLDKQSISYSLSLLLEGSEQKIKELEGTIVI
                     TQRGKAVQPKTLGQKEYIKLIENNDITFGVGPAGTGKTYLAVAMAVKAFKRDEVSRII
                     LTRPAVEAGESLGFLPGDLKDKVDPYLRPLYDALFEMLGADKFNKYLERGTIEVAPLA
                     FMRGRTLDNSFIILDEAQNTTSEQMKMFLTRLGFGSKAVVTGDVTQTDLPQNKKSGLI
                     QATEILKGVPGIGSIMLTDRDVVRHELVQRIIRAYEKHDKREEFKKEERKKAKMQEKT
                     FKRK"
     gene            10572..11033
                     /gene="ybeY"
                     /locus_tag="L3M60_000285"
     CDS             10572..11033
                     /gene="ybeY"
                     /locus_tag="L3M60_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBN6224992.1"
                     /translation="MDLILDDRQDKLEVSEELLEKIKDIILECLDYEGYDDNYEVSLS
                     FVDNKEIHELNREYRGVDRVTDVLSFPLLSDDFEDVELEEESLGDIVVSLERALEQSI
                     EYNHSFEREVCFLICHSMFHLLGYDHDTDENTKEMREKEEHILNKLNITRE"
     gene            11039..11758
                     /locus_tag="L3M60_000286"
     CDS             11039..11758
                     /locus_tag="L3M60_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBN6224993.1"
                     /translation="MKPEKTRQGIIKAFNAAIEGILYTFKFERNMKIHYLGSVAVLII
                     SLFFNFSKLEMIMLLMSICLVVVAEMFNTAIEKAVDLVTDEYHVLAKIAKDVAAGGVL
                     VAALNSVVVGYILFYDKLTDISGILIYKIRESELHITLICILLVLIAVVVVKALTSTG
                     TPLKGGMPSGHAALAFAIATAITLMTERVVASTLAYIMAVLVAQSRIEGKIHTFWETI
                     AGALLGVLIAILVFQLGMFYN"
     gene            11877..12278
                     /locus_tag="L3M60_000287"
     CDS             11877..12278
                     /locus_tag="L3M60_000287"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBN6224994.1"
                     /translation="MDNIELLRLAEDARQHSYAPYSSFRVGAALLTKSGKVYTGCNIE
                     CASLGGTNCAERTAIFKAISEGDKDIYKIAIASDNSENNEQTYPCGICRQVIIEFGSD
                     IKIITGYTKGEVFEHDIKDLLPNYFSGNDLK"
     gene            12343..13236
                     /gene="era"
                     /locus_tag="L3M60_000288"
     CDS             12343..13236
                     /gene="era"
                     /locus_tag="L3M60_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBN6224995.1"
                     /translation="MFKSGFVSIVGRPNVGKSTLMNNVVGEKIAIMSDKPQTTRNTIQ
                     AVYTDEEMQIVFLDTPGIHKPKNKLGEFMVKAATEAFKNVDLILFVVDDSKKIGPGDR
                     KIIEDLRSVKTPIILVVNKIDQLDQKDELFDIIKMYDREGIFKEIVPISALKGKNTDT
                     LIKVIQNYLEEGPKYFPDYMITDQPERVLIAELIREKVLHYLNDEIPHGVAVEIEKMK
                     ARNDKEIVDVSAVIYCERDSHKGIIIGKNGRKLKGIGKSARQDIELLLGSQINLQLWV
                     KVKENWRNLQNYINNFGYNDK"
     gene            13248..14627
                     /gene="mgtE"
                     /locus_tag="L3M60_000289"
     CDS             13248..14627
                     /gene="mgtE"
                     /locus_tag="L3M60_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HBN6224996.1"
                     /translation="MDRKLDASQDLLYEVKSLIDNNKVLELRELIEEYHIIDIFDIME
                     NLEEDMKIQLFEVLPLDMASSILEEGSVEFFISILSKLDVEHSKNILELMSLGDMADK
                     LSELEEEQREHIINLLNQENADYVKELLFYDEDSAGGTMTTGYISINKDMTALEAIDH
                     MREEAEEAETIYYIYVVDDEEKLVGVLSLRELIVARDANIVEDLMSENIISVYVDEDR
                     EEAVRLVSKYNLIAIPVVDRQEKLKGIITVDDIIDVMEEEATEDMYKFAGSSEHEREV
                     AEKENPTLKEQIISALRGRLPWLIITLVGGLLASLILSNLDYIMNPVYAPLVFFIPVV
                     IGMGGNIGTQSSSVTVITLSNKDLNFSNVVREGIVGIITGLLCSIITGIVIYFVMRKL
                     DIVLIVSISLFINMVLGATIGAFMPVLLKKMDADPSTVSSPIISTALDITGIAVYFII
                     TTALLSKIV"
     gene            14700..14855
                     /locus_tag="L3M60_000290"
     CDS             14700..14855
                     /locus_tag="L3M60_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HBN6224997.1"
                     /translation="MNNISWEVFKKTGSIDAYLYFCDCKNIHEEIEEVREKKEDDDNP
                     EHPGDST"
     gene            14821..15594
                     /gene="recO"
                     /locus_tag="L3M60_000291"
     CDS             14821..15594
                     /gene="recO"
                     /locus_tag="L3M60_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBN6224998.1"
                     /translation="MIILNTQGIVLKAIRYKESDIILTLFTRKLGKVSAIAKGAKKNK
                     SSLLSSSQLFSYSNFTLKKQGNMYKVTQSEIIKSFYNISYDIEAFSYATYITKLVENS
                     ILENQTNNRLFILLAQTLYLYTQDNTDNRFITAAFELKFLDYIGFKPIVNKCINCECK
                     ILQNSVFNIYEGGILCSKCSKLFDNNIKLDLTTVSLMEYVLRNDILTCSKAKVSKYIT
                     HELENILDKYLKVYVDNINFKSLHVLQSVKNNKGVDNDE"
     gene            15587..16186
                     /locus_tag="L3M60_000292"
     CDS             15587..16186
                     /locus_tag="L3M60_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HBN6224999.1"
                     /translation="MSSNITIEMVDSVFERVPGATYAEAKEALVMCNGDVIEAVIYLE
                     NKKNTCNCKAKSAKETMEEVFGKDGENIKFQLKELLRKSSVVRIIVEKDGKTMMNIPL
                     TVGVVGLAFVPLATLVGLSAAVISKYRIKVQNEEDGEIVDLGELNEEKLHVLKNMITN
                     AAKDVKDVVVDKKEDDKDVTADLMKEESEKNKEDEDENK"
     gene            16346..17224
                     /gene="glyQ"
                     /locus_tag="L3M60_000293"
     CDS             16346..17224
                     /gene="glyQ"
                     /locus_tag="L3M60_000293"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBN6225000.1"
                     /translation="MNFQEMILALQKYWSKQGCIMMQPYDIEKGAGTMNPNTFLRSLG
                     PEPWQVCYVEPSRRPADGRYGENPNRLYQHHQFQVILKPSPDNIQELYLESLKEIGID
                     PSEHDIRFVEDNWEAATVGAWGLGWEVWLDGMEITQFTYFQQVGNIECELETGEITYG
                     LERLAMYIQEVDSVYDLKWNDKITYGEVFNKAEYENSMYAFELCDADMLFNLFDIYEK
                     EALRLMENGLVIPSYDYVLKCSHAFNTLDARGAIGVSQRASFIGRVRNMAKTVAETFV
                     KQREEMGFPLLKDGDK"
     gene            17224..19290
                     /locus_tag="L3M60_000294"
     CDS             17224..19290
                     /locus_tag="L3M60_000294"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBN6225001.1"
                     /translation="MKNYLLFEIGVEEMPSRFVGSTLEQLKNNLSKLFDENRIAFDKI
                     NAYGTPRRLTLVVEGLSDRQSDLEEEIKGPAKKIAVDAENNLTKPALGFIKSKGLSES
                     DIYFKQVGKDEYIFATIKQDGKETSEVLKDILPDTIKSVVFPKAMRWGGKNLKFARPI
                     RWIVTLLNDKVLEFDLEGIVSSNVTKGHRFLGESTIEVDSIEDYFEKLEKNYIVLDQN
                     KRKEMIRKQCIEVANSLGGEVEFDEDLLEEVTYLVEYPTAFYGEFDVDYIKLPKEVVI
                     TPMKQHQRYFPVLKEGKLLPNFIAVRNGDSYRIDNVKAGNEKVLEARLADALFFYRED
                     TKRNLESYIEKLKTVVFQVKLGTIYDKTLRLEKLSADILDSLGLVDEKADTIRAAKLS
                     KADLVTGMVGEFDELQGFMGKEYAKVGGENDVVSEAIFEHYLPRFAGDILPKTNAGVA
                     LSIADKLDSIVGFFAIGIQPTGSQDPYALRRQALGILSILMDRKVAVDIKVLIEHALD
                     NYSELDFNREEVVEQIMNFFNERVKNLFKDLGIRYDVVDAVLSSDINDVSDMHMRALE
                     LNNWLEKDELVEMLTAFNRVSTLAQKAESDKIDESLLKEDAEIKLYNEFKQVKVRVKQ
                     LLKDKKYGVALDEFASLRPVVDNLFDTVMVMDNDEAIKNNRLALLKQVYDTMLEICDL
                     SKIVYK"
     gene            complement(19457..21004)
                     /locus_tag="L3M60_000295"
     CDS             complement(19457..21004)
                     /locus_tag="L3M60_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HBN6225002.1"
                     /translation="MDNFKKILLNEIPNFKEYSSKFLNGEINKMQYKGFSGGYGVYAQ
                     RDKKSFMIRLRVSAGVLSHHQLNKIYEMAIKHNLDKIHLTTRQAIQLHDLSIDSIVDI
                     MKEGIKNDIFTRGGGGNYPRNVGLSPLSGVDPSEAFDVTPYAVATDKYFIKNATTYHL
                     PRKLKVSYSNCYTDTAHCSIQDLGFVATLKNDEQYFRVFLGGGLGKQPKVALELDELI
                     KPKDALYCVEGMIKFFMDYGNYENKNRARVRYMVESLGEELFLKKFKEYYKLEKENGG
                     LELNIEEIDYSKSGVKICIQDSRLIPQKQDGLYTVYIHPVGGILLTKDLNTLLKELDN
                     IENPMIRLGMTEGLYILNLNGNEAKRILEISKTISCNSQLEQSISCIGVPICQMGIQN
                     SQKMLHEIIDYFRLQNNEEILNKAPKLYISGCLNSCGVHQVGSIGLCGKKKNVDGVST
                     DAFELFVGGSFEIGKTRLGKSLGDFKASDIPEMLYKISDASTGNFYEWVNSNDDILNK
                     ITDKYKI"
     gene            21284..22051
                     /locus_tag="L3M60_000296"
     CDS             21284..22051
                     /locus_tag="L3M60_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBN6225003.1"
                     /translation="MGEIINALDRALDIILLLYHEKREMGITEISKAMGVYKSTVHRT
                     LVTLENKGFVIQNAENGKYWLGINLYAIGMVVGEKMSLTEIVKPYTKKLNQEFNEVVN
                     VSILEERAQDSPRSIIIHKEYGSNQLLSVNPSVGSSSECYCSAVGKCLMAFNDSIDFE
                     KYRKTPIHKYTEHTIDNWDDMMLFLAKIKEQGYAIDDEELEHGLTCIGAPILDKNNKA
                     IAAISLSGPTIRMREGDFEYKIKRVIETAKSISELFR"
     gene            22336..22533
                     /locus_tag="L3M60_000297"
     CDS             22336..22533
                     /locus_tag="L3M60_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6225004.1"
                     /translation="MEKVKLSVERCKGCYLCIEACKKDAISLSDYTNKKGFVTIKVDE
                     EKCIQCGACYTMCPDLVFEIL"
     gene            22562..23638
                     /gene="vorB"
                     /locus_tag="L3M60_000298"
     CDS             22562..23638
                     /gene="vorB"
                     /locus_tag="L3M60_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBN6225005.1"
                     /translation="MQSLLIKGNEALAEGAIRGGCRFYAGYPITPQSEILEMMAWRQK
                     EVGGVFIQGESEIASVNMVMGASALGARAMTSSSGPGFSLKQEGIAYWVSAGLPAVVA
                     CVQRYGIGDGFIGAGQDNYWQAVKGGGNGDYHLIVLAPNSVQENMDMAYESFELAEKY
                     MHPVLILSDATIGQMVEPCIAPPMKEYDMDKAPWAAKGTPVGEKNKVITDITYFEDQD
                     TWQKGYIEKMRKVQEDEQRWEEIEIEDAEVVFVAYGISSRIAEGAVRDARAKGMKLGL
                     IRPKTLWPYPRKAFKKLSDSLKGLVTVEMNMMAQMKEDVIIACDNRFPVYSVATAQYM
                     AEVNQLIDFAQQIIDGKAKQEEVF"
     gene            23641..24360
                     /locus_tag="L3M60_000299"
     CDS             23641..24360
                     /locus_tag="L3M60_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBN6225006.1"
                     /translation="MAKKAPAIMMCENMFCGGCGHSIVDRIIGEVLTEMDICQDTIIC
                     SDIGCNHMFQFSMNVDVVVPPHGKMGAAMAAMKRVRPDKYIFTIAGDGGAYAIGLGET
                     MSAATRNDNVIFLVMNNNMFAMTGGQMAPTTVEGQYTASTPAGRNYADHGSTFDVVKV
                     MGNLDIAYLARGTITNPKEINTTKRYIKKALEKQKEKKGFCLIEVLVPCPTNWGLKPV
                     DAMKRIENELVPKYPLGEFID"
     gene            24373..24912
                     /locus_tag="L3M60_000300"
     CDS             24373..24912
                     /locus_tag="L3M60_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBN6225007.1"
                     /translation="MLNEIICAGFGGQGVLTAGKIIMYVAYKAEKKITWFPSYGNEMR
                     GGSANCNVVVSDEKIANPCVKNPDILLGMNSVSVDKYESLMKKNGYMFVNESLVSKDR
                     KYREDINVIRVPVTEIAQNLGNEKAANICMLGAMVKNLDLFTKEEFENGMCEYFENQG
                     KGKFNSKNVEAFEAGYDYE"
     gene            25069..26127
                     /gene="buk"
                     /locus_tag="L3M60_000301"
     CDS             25069..26127
                     /gene="buk"
                     /locus_tag="L3M60_000301"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBN6225008.1"
                     /translation="MKILVINPGGTSTKISVFKDENEILKKNITHTREDLKNFSKVFD
                     EYDYRKQLIVDILSSENHSINSFDAVVGRGGLMKAIKGGTYTVSEEMIEDMRNEINGE
                     HASNLGALLAKTIADEIGVQSFVVDPVSVDEFEDVSRITGISDIEKASWLHALNHKAV
                     SRKIAQKLGGKYEDYNFIVAHLGSGISIVAHKQGKMIDGSGGRSDGPFSPERSGGLLA
                     YPLIELCYSGKYTHDEMVAKVSSIGGMYDYLGTKDMIEIENRIKQGDEKAKLIMDAFI
                     YQVAKEISMYGASLSGNVDRVILTGGISHSEMVVKGVIEKVSYIAPVEVVPGEMEMEA
                     LALGALRVLNGEEIAKTY"
     gene            26145..27038
                     /locus_tag="L3M60_000302"
     CDS             26145..27038
                     /locus_tag="L3M60_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBN6225009.1"
                     /translation="MLKDFNNLLEEAKKLEPVIVSVAVAEDKEVLKAVKSATDLNIIK
                     PILVGDKDKIENISKEIGLKGHEIIGCQNPDESVKKAVEIVKNKEAKVLMKGLVNTSV
                     YMRAILNKETGLRTGRLLSLLAVYELPQYHKLLYCTDSGINVSPNLNQKKDIMVNALL
                     TMKSIGMENPKTAILTANEMVDPKIQSTVDANLLVEMEKSGEIPNCIVEGPIAFDVAF
                     DAHAAEHKGIKSNIAGDVDLLVFPNIEAGNILGKSWLRFNKAKWAGIVLGATNPIILG
                     SRSDTAEIKINSIALACLASQ"
     gene            27068..28276
                     /locus_tag="L3M60_000303"
     CDS             27068..28276
                     /locus_tag="L3M60_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN6225010.1"
                     /translation="MKYAKRMDMVKASAIRDSQKKIAQKVASGGTVISFAAGLPDPNL
                     FPIEEISEVTQKVLKERGKFALAYGPTKGEDELLELLVKRMKEEENIDTKAENIIITT
                     GSQQGIALSAMILLEKGDVVVTENPSYLGAINAFRPYECDFLGVDTDEEGMVTDDLDK
                     LLSENSNIKVIYVIPTFQNPTGKTWSLQRRKDFMEVVSKYDVIVIEDNPYGEIRFTPE
                     PLPTLKSLDTKDKVLYLGSFSKVLCPGFRVAWMCGNVDIIDKAELLKQGIDLQSNQFS
                     QVQVIEFLKKYNLNEHIEKIREEYKKRCLLMLDAMKKHFPEEAKYTEPDGGMFIWVEL
                     PESINVDDLLDKAIDAGVAYVSGESFFANNGPKNTMRLNYTTMSEEQIVKGVEILAEV
                     IKKELNYACE"
     gene            28300..29559
                     /locus_tag="L3M60_000304"
     CDS             28300..29559
                     /locus_tag="L3M60_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225011.1"
                     /translation="MGEKENGIKFGKLMFQLVIIAISWELVYIIPFIQYTLYDPILKA
                     LECTNTQLGFLLTIYGLGNIFGAPIGGWLADRFDYRKIILGSVFLNGVVSFLFAFNMN
                     YPFAVATWVGCAITSLVMNYPSMVKILRVIGKDNQGKVYGFNEAMVGVSGVVMGAIFL
                     YIYTCFATPTLGMRWVMISLGILSIAMCPVLWFVIKDVDIKEEKEEAGEKMSAGDFIT
                     VLKSPNTWLVGISIFCVYSFTVTMSYFTPYITSVLGGSVALSGALAIIRQHGLKLFGA
                     PFGGYCADKLKSPTKVLLPIYVFGIAVIVLFLVLPASTPMTIFIALTFVVGILGYMGK
                     GIYYAVQDEVKVPVKYSATTIGIAAALGFSPDVFQFALIGHWIDTYGNKGYTYTFIFQ
                     IAILVIGILSCLYILKVKKRRASKLEA"
     gene            30204..31667
                     /locus_tag="L3M60_000305"
     CDS             30204..31667
                     /locus_tag="L3M60_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBN6225012.1"
                     /translation="MTDLVNTINGIVWSPVLVAMCLCIGLYFSLKLKFFQIRDVKEMF
                     TLLLEGDSSHVGISSFQGFATSMAGRIGTGNIAGVAVAIAMGGPGALVWMCIMATLGS
                     ATAFVESTLAQIYKDEHDGQYRGGPPYYFEKGLGWKKCSVLFSIVAIISYTFFMPGTQ
                     SNTLYLAMNQAFGFSKMTIAVASSIALAIIIFGGIKRIGLFAEKVVPIMGGAYLIMTL
                     VIIITNISLVPEAIVTMFKSAFGFGSVFGGMLGSAVSWGVKRGIYSNEAGEGSGTYGA
                     AAAEVSHPAKQGLVQAFSVYVDTLLICMATGIMIVITGMYNVTGVCTTLPGVEPGPQY
                     VQSAINTLHPQLGDIFIAIVLLLFTFTTLLAFSYMMETNVSYLVTRIKAEKYEKYIIN
                     VCRFMLIACAAMCCFIEPLTSWALGDICIGVLTYINLIAIFTLKKPAIKAYYDYIRQK
                     KMGIKRSDRTFNPIELDIRNAEFWENRYLKENKHELN"
     gene            31723..32859
                     /locus_tag="L3M60_000306"
     CDS             31723..32859
                     /locus_tag="L3M60_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBN6225013.1"
                     /translation="MKERIIQVADSKKEKILGLCQSLYDEPEIALQEYKSAKKIAEFL
                     REEGFDVEENLAGMATAFKATKKNGDGPKIAFIAEYDALPGNGHACGHHLIASMGVGA
                     GIALSSILDTYKGEVSIIGTPAEETGDGKPYLIEHGVFDGYDAAMMIHPNSKTCVTPE
                     IIAIGGLDFIFTGKASHAGAKPYNGINALDAVVLLYNNINALRQQLIDGTRIHGIILE
                     AGTAANVIPDMGKVRLEIRAKEQNYFDEVVEKVKNCARGAAIATGCELEFYHFEPTCQ
                     GLNENKVLVEIFTKVMEEFGIYEDEQVLLGSTDMGNLSQIMPCIHPLMKFSENGEELH
                     TKEFLEASINPYAKDRVIDGIKILALTGFNLFENSELLKKMKEE"
     gene            32884..34302
                     /locus_tag="L3M60_000307"
     CDS             32884..34302
                     /locus_tag="L3M60_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBN6225014.1"
                     /translation="MTNKTEGKKKKLTFPDAYVLMIGLIVFAAIMTYIVPSGTYSRIE
                     DLNTGNMLVQAGSFKYGEQHPVSIMDVFRAIPSGLGKNSVTIFFVLIIGGAFGIVNFT
                     GSLDVLIGRVISKSQGSKKGEIIITSLVLTFGLAGGVVGMYEECIAFLPILMTLCIAL
                     GYDALVAAGILLLGLASGYGSATINPFTVGLAQGISGLPLFSGMWFRWIFWICTMILL
                     SGYIIFYCKKIKKDPSKSLVSDIDYSHWQLDESSIKEKLSSGNIRVLVTFFGGILVLM
                     IIIVKFSLGMSDLSAYFFALGILCGVVYGMSPNKIASAFVKGMQEMVYPAILIGFASS
                     IVVILEQGVILDSIVHGLSLVLNYVPRIFSGGVMMIMQSIINLFIPSGTGQAMVTMPL
                     MAPLADVIGVPRQVAVLAYQMGDGFANGIVPTLGVLMAGISIAHIPYTRWFKFASKII
                     LMELVLGFIFLMISVQINLGPF"
     gene            34514..35632
                     /locus_tag="L3M60_000308"
     CDS             34514..35632
                     /locus_tag="L3M60_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HBN6225015.1"
                     /translation="METLKIRNIELSGTNYEIGYELGKLIANTPKILEGQINKSNVVT
                     KEEEKEMLELFDKYCPGLNEELKGFADAVQVNCNQILYYTMTYLKPGCSQVALAPELT
                     TNGHVLFARNFDFSHNMEDFVLCKTKVKGKYAHIGTTVMQFGRGEGMNECGLGVSQSS
                     CGIPVGNSVGLRKPAVVGLQFWAVIRYLLENCKDVDEALEYIEDMPIAYNINLLLADK
                     SGNIALVETLDGKKGVNRINRLEKQREHFLHSTNHIHIDELHKLEPQSIKNSIHRYKL
                     IKEYINNSKKVGEKELMNLLSSKYPNGLSCNYYNDFFGTLKSIVMDLNTGKFNILWGG
                     LESNNWESYYLKDDVKATTHLININIEKAPSDFFDFVE"
     gene            35783..36652
                     /locus_tag="L3M60_000309"
     CDS             35783..36652
                     /locus_tag="L3M60_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6225016.1"
                     /translation="MDYLEYFEKAVIYIENNLQENITVNDVAKESGYSYYHLTRLFKS
                     MFGESVGSYIKKRRLVNSTKELLYSDKKVIDIAIGSGFESSEAFSRAFKSIYKVSPIE
                     YRKNRIDVFVGNKKKLELDFMRHLIGNITIKPVIKEIDEIKIIGIKDKVILENNSLPE
                     LWDKFRKVHQIVPNTLPSERVFGICEATSEIHLISESMEFNEIIGVEVSSYESIPNSF
                     VSKTITEGKYAVFTHTGSLENLDKTYEYIWGTWFLNSKEEFDTRDDFEVYDERFLGPN
                     NINSQIDIYIPIK"
     gene            37027..37572
                     /locus_tag="L3M60_000310"
     CDS             37027..37572
                     /locus_tag="L3M60_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBN6225017.1"
                     /translation="MENVITGLSKGAEWFIGLFQKGGEQFIGLVSGTLPTLIVLMVAI
                     NSLIKIIGEERVNNWASKLGKNSFTRYILLPLVSVFFLGNPMCYTFGRFLKEEHKAGF
                     YDAAVSFVHPITGLFPHANAGELFVWLGISAGLTTLGKETTTLALWYFIVGLIVIFIR
                     GIVTEKMYAFLTRKNKVADKA"
     gene            37585..38598
                     /locus_tag="L3M60_000311"
     CDS             37585..38598
                     /locus_tag="L3M60_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBN6225018.1"
                     /translation="MDENKILKIEKGTSGWGGPLYIKKEGNRNKILSMTAGGIHEVTL
                     KIKELLGCEVVDGFKTGVSDEEVAVVIIDCGGTARCGVYPKKKIPTINVNPVGKTGPL
                     AKFITEEYYVSDVSPNCISVVNGEDMQKKSQENKSENKSSIRKPDNYDEVKSKAQGEY
                     AKKNIILSIGQGAGQVVSKFYDAGRDTIQMVMNNVIPFMAFVSMLMGIILASGLGDWI
                     AKVISPLAGNIGGLLIISIICTLPFLSPILGPGAVIAQVVGTLVGTQIGLGAIPAYLA
                     LPALFAINGQAGCDFVPVGLSLGEAEPETVEYGVPALFYSRLITGPISVIIAYGVAVF
                     ALR"
     gene            38820..40793
                     /locus_tag="L3M60_000312"
     CDS             38820..40793
                     /locus_tag="L3M60_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBN6225019.1"
                     /translation="MSVTEKIPSIDHKIFKILIMCSKKEFVSINSIANELNVTTRSVR
                     TYIKQLNKDLGNDIAIIKYIKGQGYKLEIKDEQILNKIIDINRKNVFSLNSKEDRVEF
                     ILNYLTELDGFITLDSLADEMCVGRTTLVNDFQYVEKVLASYNLNLIKKQNTGMKLNG
                     NELDIRLFILNQLYKNSRKDFNNSKYFKGIKKEEIINLEEKLLTLFKKNNFYVTDEML
                     REVINYIVVLVYRVKETKKVKDYDVKFDLLKSYDEYFIAREIKHIISEMFECILNDEE
                     IIYLTIPLVSGNAPASECALNSSRINKNIDELMESIFNQIYVDMGIFINEDELRVGLG
                     YHLSFTLNRLLFNIKLKNVLLEEIKQNYILPFKLAQIAGKVIEKKYNLEVSEDEIGYI
                     AIHFSGYLERNSSRFYSIKKIAIICSTGLGTAKLLKIRVEKLIGNNPKIDTLSSFNLR
                     NINLDEYDIVFTTIDLDTSNINTIVLKINTIFDENKLREQLKTVLCLREGNIDTTNST
                     NLLINNLLDEDKFMILNEKTILDSLEKMMDNLMDLGCIDDKFRKNIFNREEKSPTVFD
                     KGLLFPHSVNEKSDKFLMAVGILEEPIKYANRNIKIILMTMFPCENKMDSDLLVKIYE
                     EILKIGQDVKLTNKISKCRSFLEFKKVLLKNLM"
     gene            40863..41222
                     /locus_tag="L3M60_000313"
     CDS             40863..41222
                     /locus_tag="L3M60_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225020.1"
                     /translation="MKVLVILFAAYILNTILIMKQNKLYFKTLNRAKKKGDIVSTGKK
                     KSYFSKGSIAIISSDSNGFIKFGEILKGRTVMAKFKEIEDIEGLDIYSAEQKFKNEES
                     IVQAIKFIKEKINVSFN"
     gene            41257..41613
                     /locus_tag="L3M60_000314"
     CDS             41257..41613
                     /locus_tag="L3M60_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBN6225021.1"
                     /translation="MKYEVKISGIGSLVKELMDESNCLIIYDETINDDDLKDISVIHS
                     ISTLRSDVEIGDTLTIGNRDYCIVSVGDIAQKTLREIGHCTIKFDGKNEVNLPGEIHV
                     EIGNPDITIGDLITIS"
     gene            41650..42942
                     /locus_tag="L3M60_000315"
     CDS             41650..42942
                     /locus_tag="L3M60_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBN6225022.1"
                     /translation="MLDLNRKLAKLEEEGKKIRVALIGAGHMGNGMVSQMANMKGIEA
                     SIVVDINLELAHKAFTDAGIDEEIIDNVTNENDAEIKLQEGKVLTCNDFLVACKTKSI
                     DVVIDATGGIAIGAEIALNSILNKKHIVMLNAETDCVVGPILKKLADDAGVIFTGSAG
                     DEPGAVMELFDFADAMGFEVRVIGKGKNNKLDLDCNPDTVREEAEIKGASPHMIASFK
                     EGTKTMVEMALMCNATGFVPDVRGGHGIEATVNEVPKKYALKSEGGVLDNYGVVDFVN
                     GIAPGVFVVVAHKLKAVNDELKYLSMGDGPNYILYRPYHLCSLETPLSAAMAVLEHKA
                     TIVPKAGLVAEVMTIAKKDLKKGEYMDGYGAYTCYGTIEKYDVAKAMKAVPIGLISKK
                     TKVVKDIKKGEVITYDMVEIEKDTTLYHLRQLQEKIFG"
     gene            43378..44016
                     /locus_tag="L3M60_000316"
     CDS             43378..44016
                     /locus_tag="L3M60_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225023.1"
                     /translation="MIIIQLNERQLKIIDIVKENEPITSESIASSLNVTRATLRSDLA
                     ILTMTGILDARPKVGYFYSGVSEINLIGKSVKEKTVEDIMSMPVLAKKDESIYDVIVT
                     MFLSDVGSIVIIDENEELCGVVSRKDLLKATIGGSDINKMPIGMIMTRTPNVVTLTKG
                     ASVLLASRKIIEHEVDSIPIVEYKGEDKNHMRVVGRISKTNITKLFLEIVDN"
     gene            44023..44865
                     /locus_tag="L3M60_000317"
     CDS             44023..44865
                     /locus_tag="L3M60_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HBN6225024.1"
                     /translation="MLVKNLIIYAVSDSVGETAQQVAKACMSQFYVNETYEIKRFPYM
                     INKGVLLETLENAKAENALIVYTLVDEELCSIVERYCEREGLSCIDLMTDILREISKR
                     TGRKPKREAGIIRKLDESYFKRVEAIEFAVKYDDGKDPRGILQADIILVGISRTSKTP
                     LSMYLANKNIKVANVPLVPEIPIPKEVFEIDTKKIIGLTNSPEKLNEIRTQRLKALGL
                     SSKANYANLERILQELDYSEEIMKRIGCPVINVSNKAIEETAGIILDIMKENGLKIYK
                     EIEI"
     gene            44926..47553
                     /locus_tag="L3M60_000318"
     CDS             44926..47553
                     /locus_tag="L3M60_000318"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBN6225025.1"
                     /translation="METKYVYSFGEGSKDMKSLLGGKGANLAEMTKIGLPVPPGFTIT
                     TEACNDYYVNNESIRKEIIKEIEEHLATLEKDLNKTLGCNKNPLLVSVRSGAVFSMPG
                     MMDTILNLGLNDNSVVGLAEATQNERFAYDSYRRFIQMFSDVAMEVPKYKFENVLDRV
                     KEAKGYTVDTELTTDDLKEIVKEFKAIYKKEIKNDFPQDPKEQLMLAIEAVFRSWNNP
                     RAIVYRKLNDIAHNLGTAVNIQSMVFGNMGETSGTGVAFTRNPATGENKLFGEFLMNA
                     QGEDVVAGIRTPQNISTLADVMPAVFDEFVKITHILEGHYKDMQDIEFTIENERLYIL
                     QTRNGKRTAAAAINVAVDLVEAGIIDEKEAIMRIEPNQLDQLLHPKFEDNALKEAKVI
                     AKGLPASPGAASGKVYFNADDVVKANEKGEKVVLVRLETSPEDIEGMVKAEGILTARG
                     GMTSHAAVVARGMGKCCVAGCGEIKVDEFNKEIRALDDVVIKEGEYISIDGSTGNVYL
                     GDVKKTEVSLTGNFEKLMNWVDKHKCMMVRTNADNPRDARAAIEFGAEGIGLCRTEHM
                     FFDEARLPAVREMILSNTVEQREKALEKILPMQREDFVELFKVMDGKPVNIRLLDPPL
                     HEFLPHDDETIEELSKSMGIKVSDIKKRIVDLDEFNPMLGHRGCRLAITYPEICVMQS
                     KAIIQGAIEAIKAGVKVSPEIMVPLVGEVNELKIIRKMIVETVDAIIKEEGVEVPYTV
                     GTMIEIPRACLTADEIAQEADFFSFGTNDLTQMAFGYSRDDAGKFLGQYVDEEILEKD
                     PFQVLDQNGVGKLVKMGAKLGREVKPELKLGICGEHGGEPSSVEFCYSVGLNYVSCSP
                     FRVPIARLAAAQASIKNPR"
     gene            47767..48012
                     /locus_tag="L3M60_000319"
     CDS             47767..48012
                     /locus_tag="L3M60_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN6225026.1"
                     /translation="MTKCPKCGKEVSSRPGSICPRCGFKVADESLKIRCPEPSCRALV
                     SRKLDYCPKCGCKLRGYNISEFVNMVRCKIDETFDKR"
     gene            complement(48302..49612)
                     /locus_tag="L3M60_000320"
     CDS             complement(48302..49612)
                     /locus_tag="L3M60_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HBN6225027.1"
                     /translation="MNKKVSKIVAIGCGVGIITANLIPVYADNSTSSINKVDNSIVSD
                     STKRTSEKEILTAREAVSAALKNSEKLKMKSEEIKMLKEKLEVQDEFDSFTGSDNSFP
                     YDQIGLLKNQSEQAKGFMEDQIANDITNKFNDLVSRENELDKIKNNLEIKTKEIKDMK
                     LKKDLGLVTSLETESAELELQTLQNTQKAKLQELKNNQDYFKLLTNIDLNNYQLDKEY
                     RFESFRVGGSVDSYMEGKVNEYLKYDQLILERTEESFNDKDENKADLPDRPNLTRPVA
                     PTKPEQGNLTDDEYKVLMDKYGKDYDRYKKEVEEYNLERQTYAAGLTTYANYLQQKYN
                     TENGLVTLEDNKKALKKGLIDSYAQLLALEDTIQITKKQLELSEKQLKNTKLRYDLGL
                     ITLTDYKKQVVSNEDAKNNYDTLIVNYNSLKNGIEKPWILNTGK"
     gene            complement(49634..52768)
                     /locus_tag="L3M60_000321"
     CDS             complement(49634..52768)
                     /locus_tag="L3M60_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HBN6225028.1"
                     /translation="MNLTQTSVKRPLTIIMVFLVLIVFGGIGYKKMSINLMPDIEIPV
                     VMVMTTWTGAGPQDVDEQIGQKVDESLSAVSNVKSTISSSQESVSMVVAQFEFGTNLD
                     EIMNDVRSKVDALQTSLPDDAAKPTVLKLDMNAQAIGQLVISGGNENSSQALRKYAED
                     VIQPKIESIDGVTSADLKGGEKAQVNVIADPAVLSNYGVSLSTIKGVLSSSNKTFPYG
                     SITQGEDKIVLRAIDKLESLDDIKQIQIPVKGGNTVRLDNLCSVDYGYADKDSIYRYN
                     GKDSLVIDISKQQDANTVKVMQSAYKYVDELNKENPQFKIKIANDTSSYISESINSVM
                     NNLLISSVIAFIVIFAFLKSVRASLVVAIAIPISIIGAIAILYFTGESLNLITASSLV
                     ISVGMVVDNATVVIENIFKYRKSGRLSIDDCAIEGTRTVTNAILASTLTTIAIFLPIM
                     FTDGIAKITFGALGKTLIASLAFSFIGAITLVPSVFAKLSRGKNSQKMQEKDTPLFDK
                     VSELYKRLLSVCLRHQRLVVISSLLIFIASLFGMSFIGMDFMPAADKGELAISIELPK
                     GLSLESNDYYVSMTEKKVSDIPEIKTLITTLTSDSNNNTANIAIELESQKNRKRSTDE
                     VEKEIIDRVATVPDCKINVSQNSSVMGGTSGADFTLEMKGPDLDVLKILGKEAEKQFS
                     SIKGFRNVETSLTDTTQEAQFTIDKHKAQEYGINTAEIAGLLRTAINGDSVTTATIDD
                     YKVDVNLKFKADSIDNLEDIKQLKVNSTTGKEVPLGAFSDIKMADGLQSISRTDGNFS
                     VNITATLDNLDAGTATKLALEKTNKMVIPKDYSIGVGGQTEMMNESMASLGFSMLIAI
                     VLVYMVMVAQFESFSKPFIIMFCVPFAFVGVVLSLLVSGQSLSTVGMLGIILLIGIVV
                     NNGIVLIDYIEQLRKTNRNNDLIGLVSIGCAARLRPVLMTTATTILGMLPTALSLGEG
                     GETMQPLAIVIMGGLTISTLITLVLIPTIYLIFDKWENKFSDKMPKIFGKITDKIGDI
                     KRKIIKKPKLKAKV"
     gene            complement(52772..53995)
                     /locus_tag="L3M60_000322"
     CDS             complement(52772..53995)
                     /locus_tag="L3M60_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBN6225029.1"
                     /translation="MKRYLIVFLMCSSLFLVGCGKTEEPEPEKPIAVSVQKAVGGEIE
                     NTNAFSGTTKVKDETAVTAQTVGTVQEVYVKLGQNVRKGDELLSISSPDLENSVKQSK
                     AALDLAKASYSSTTGGSLEAQVNQAKTALDNAKIQYDESQRNYDNNKILYEQEVISLD
                     QFKKIEFALEQSKQQLDSAQRSYDTVTSKSIPQAKALAKKQLDQAQVSYNLAMSNLDK
                     LTLTAPVDGTITAKNFDAKEMITQSQPAFIISNPNILEVNLNVAESDIDKFKKDGNVE
                     VIIEDQRILGKIDYVPSVVDPQTSLYPIKVLVNNTNNKFKAGMSAQVNLSVEKENGAV
                     TVPKKAIFEEKGKKYVYIANKDNIAKKKLVQTGIVTEDKIEIKSGVSEKDTVVIGGIS
                     LISDGTKIFPVEKEK"
     gene            54247..54732
                     /locus_tag="L3M60_000323"
     CDS             54247..54732
                     /locus_tag="L3M60_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6225030.1"
                     /translation="MDNNNILKIADSFLDFLFVLENNIFRESDLLKKFQNNSDIMKAY
                     FEECPMAPSHAKVILYLMTSNSSSISQIASNLGILKSNMTPIIDRLVEHGLVNKFPDP
                     KDRRILRVELTDKAFELFGAVKAILKESLVKKLSNLSEEELTLLDEHTLKLSEIVKKL
                     G"
BASE COUNT        21006 a         6154 c         9927 g        17691 t
ORIGIN      
        1 actgatgaag aaattgataa tatgatttta gatgagatat ataagagtca gggtaattta
       61 gaaaaaaata atatagagtt caatactata caaaaaaggt tgagagaact ttctggaaga
      121 aatgttctga ctgttgcatt tacaggttca ggaaaaaccg ctgcagatta tagaaagact
      181 tttaaaagaa aattcttttt agtaccaaat aaaatatcag cagaaagttt ttatagaaaa
      241 aatatttttc aaaataaaaa tgatttaaat acaagaaata gtaatgatta tattggatta
      301 cttcatggag atataaactt atactcagag aatgaagata atggagaaca tgattttgta
      361 ttgacattaa gggatataga tttgagtata aacttttgta aaccttgtgt aattgcaaca
      421 gttgaccagt tattacttag tatgtttaaa tttccgggat atgaaaaaat atttgcagct
      481 gtaaagaatg catctataac tgttgatgag gtacatttat tagaaccaaa gatgtttttg
      541 attttgatat attttatgca gtttgcatgc aagtatttag atgttgattt tcatttaatg
      601 acagctacac ttccaaaatc atataaagag caaatgataa acaagggaat tatttttcaa
      661 gaagaaagta atgaaaatgt tactgataaa ggagaaatag cgtttattga gagcaacaag
      721 gaagaaaaca tttgtgaagg taaagatgta aaggtttctt ttataaagga aaaagaaatc
      781 aagtcaatag ttgaaggtgc tttagaaaat aaacaaaaga ttcttataat aaagaatact
      841 attgattcag taaatagaac atatgagttt ttaaaagaaa atttatcaga taagtatagt
      901 ggtgtagata ttgatgtttt gcatagtaga tttaaattca aagataaaaa agagaaatat
      961 agtaaaatat taaatggaaa gggtgatatt tggatatcaa ctcaatcagt agaaatatcc
     1021 ttggatttgg atttcaatat aataataagt gatttagcaa ctatggatag tttgatacaa
     1081 agaatgggta gatgcaatag aaataataaa tatgaatatg gaaactttta tattctacct
     1141 agtgaagata aaatttatga taacaaactt aagaacacaa ctaaaagcat cttaaataac
     1201 gtaataaaaa ctaactctat ttttacaatg gggattagaa aaactatttt ggatgattac
     1261 tatgataata gtgtagtcaa aaaatatttt gaagataatt ttataagttg tgataaagaa
     1321 attaagaata tttatggtat aaataaagaa atttttgatg gattggattt aatttttaat
     1381 tttgaacctt ataaaaatat tgttgatagt aagaaggaag cagctaaaat ttttagagat
     1441 gttgatgtta gttacaaaat tattttggaa gaagattttt ataaggaaga tagggagtta
     1501 caacaagatt cgattcaagt atcagggttt atatttaata gattatatta ttatagatta
     1561 ataagcaaag tagaaggata catggtacta aaatctagta gtaagtttga gtataatagt
     1621 actgtaggct taattttaaa gtgagcatat gtttaaatat aatatgcctt tatacattgg
     1681 aaatacaaag agcactaatg taagcccact gtaaaaacta gttttttaaa agaaaaataa
     1741 gttgtgcaat agagtgttca cacgtgtata tatagcaaat ttggggttta agattaacta
     1801 agtggatttc aggtacatca tgaaaataat tataacctgg agattttgta tgtttaatat
     1861 taactaagta gatttcaaat attgatatag tagatataat agaaatttta tgattaggtt
     1921 taaaattagc taatcgaatt ttctaatatt ctgactatta tataagtata ttattatctt
     1981 gaatttaaaa ccaataatat aaaacaacaa aatattatat ttatgatata ataaaagtta
     2041 tggaaaaata ggaagagtac tatagagaag agaggtaaac ttatgaataa ttggatagaa
     2101 gtaactataa aaacaacaac agaagcagtt gagcctataa cgaatatact atatgaacaa
     2161 ggagctggag gagctgtaat agaagaccca aaagattttt tattccaaaa gaaaaatgaa
     2221 ctagattggg attatgttga agaagaagta tttaaaaaga atgaagaaga tgatgttctt
     2281 ataaaaactt acgtttctga agaaaaaaac gtgatggaat ttgtcgaaat aataaagcaa
     2341 aaagttttag gcttaaaaga ttttggtata gatataggtg aaggaagtgt atcattagac
     2401 caagtaaatg aagctgattg ggcaaatgca tggaaagcat actacaaacc aacaaaagta
     2461 ggacaaaggg tagtagtaaa acctacatgg gaagattatg ctatgcaaga tggggattta
     2521 atcatagagc ttgaccctgg aatggcattt ggtacaggta cacatgagac tactagtatg
     2581 tgcattagag aattagaaaa gtatgttaat aaagattcta aagtatttga tattggctgt
     2641 ggtagtggaa tccttgcaat agcagcagcc aaattaggag caaaagaagt tgtagctgta
     2701 gatttagatg aagtggctgt aaaagttgca aaagaaaatg ttcttgaaaa taaagttgaa
     2761 aaaagtgtat ctgtaatgca tggtaattta actgatgtaa taaaagataa agcggatgta
     2821 atagttgcaa atataatagc tgatataata aagatacttg caaaagatgt tcaaaatttt
     2881 atgaaagaag atgctatatt tatatcttca ggtataattt tggataaagt agaagaagta
     2941 aaagaaagtt taattgaaaa tggatttgaa atagttgaag ttcaaaaatt aggtgaatgg
     3001 tcagcaatag tttctaagct taaaaattaa gggatatggt gacgtataat ggatagattc
     3061 tttgttgaaa aaaataatat aaatctacaa gataaaactt gcactataga aggtgaagat
     3121 gtaaaacata tttcaaaagt tttaagatat aagccaggtg agaaacttga gatatgtgac
     3181 aagaataata atgagtatat ttgtgagata gtgaatatag ataagagtat agttaactta
     3241 aatattttag aaaaagtcga tataaatagg gaatcagaac ttaaaataag gttatatcag
     3301 ggacttccaa aagctccaaa aatggagatg atacttcaaa aattaactga agtaggtgta
     3361 gaagaaataa tattagttca aactaagaga agtgttgcta aagttgatga caaaaaagaa
     3421 gataaaaaat ttgaacgttg ggaaagaata atttatgaag cagcaaagca aagtaaaaga
     3481 ggtaagatac caaaattaag aggtgtttta acttttaaag aagctttaga agatatgaaa
     3541 aaaaataata taaatatttg tccttatgaa aacgaaagaa cagttccaat gaaacatgca
     3601 ctacaaaatt gtgactctaa tatagatagt gttggaattt ttataggtcc agaaggtgga
     3661 ttttctgaag aagaaataga acaaatacaa aagaataatt gcaatgttgt atcactagga
     3721 cctagaatac taagaactga gacagcttct gttgtagcat caacaatagc tttgtatgag
     3781 ctaagtgatt taggaggaga aaaataaatt gaaaaaagta gctttttata cacttggatg
     3841 taaagtcaat caatatgaaa cagaagctat gctagaacta tttgaaaaag atggctatga
     3901 acaagtcaat agtgaagagt atgctgatgt ttatgttata aatacatgta cagttactca
     3961 tatgagtgat agaaaatctc gtcagtacat aagacgagtt aaaaagaaga acccagacgc
     4021 aataatagct gttgtaggat gctattctca ggtatctcca gaagaaattt tggatataga
     4081 agaagtaaat ttagtcatgg gtacaaatga tagaagaaag attgttgaag aaattaaaaa
     4141 gataaattca agtaagaaag ttagtacagt tgatgatata atgaaagtta aagcttttga
     4201 agaaatagaa atcagccaaa ctaatggaaa aactagagca tttatgaaaa tacaagatgg
     4261 atgtgacaga ttttgtactt attgtatcat tccttatgcc cgaggacgcg ttagaagtag
     4321 agatattgat agtatagtcg atgaagtgaa aaaattggct aataatggct ataaagaggt
     4381 cgtattaaca ggaatacatg ttgcttctta tggaaaagat ttaaaggata gggatattaa
     4441 acttcttgat gtaataaaac aaataaacaa gatagaaaaa atagaaagaa ttaggcttag
     4501 ttctgttgaa ccaattttat ttacagatga atttgtaaat gaagttttaa agatggataa
     4561 agtttgtcct cattaccatt tgtcacttca aagtggatgt gatgaaactt taaaaagaat
     4621 gaatagaaga tacacaactc tagagtacaa aactattgta gatagattga gaagtaaaat
     4681 gccagatgtt gcaataacta cagatgtaat agtaggtttc cctggagaga caaatgaaga
     4741 gtttaaaaaa acgtacgagt ttttaaaaga aatagagctt tcacaaatgc atatttttaa
     4801 atattcacca agaaaaggaa ctccagctgc aactatggaa aatcaagttg accctcaaat
     4861 gaaacatttt agaagcgaac aacttttaaa tttgagtaaa gtaaatttta ataaatttgc
     4921 aactaaattt atcggccgta agttggatgt tttatttgaa caaaatctag aaggcaataa
     4981 atatgaggga ttaacttcaa attatataag agttgttgtt gaaagtgaca aaaatataca
     5041 aggtcagata ttaaaagtga aaataaatga tgtaaaagat gaatatgtag aaggaatttt
     5101 gctataacat gtagaaatta taatatttat aagataggag gtggaaagaa tggattgtat
     5161 tttttgtaaa atagcgaacg gagaaatacc ttcaactaaa gtttatgaag atgatagagt
     5221 cttagccttt aatgacttga acccagttgc tccatatcat atattagttg tacctaaaaa
     5281 acattatgat agtttaattg atattactga taaagagatg gacattgtgt cacatattca
     5341 tgttgttatt aataaaatag ctaaagagaa aggttttgac caaactggat tcagagtcat
     5401 aaataattgt ggaagtgatg gagggcaaga agttaaacat ttgcactatc atattttagc
     5461 tggtaaaaag cttcctaatt acgaagcggg acaaaactaa actaaattat tgaaaaattg
     5521 agttccttct tgataaaaat acttgataaa agtaaaaatt tagtttataa taaacaaagt
     5581 gttaggttgt aatgcatatt atgcaaaaag aacatagtta cagtcccagc ataattgcgc
     5641 tattggaggg agggaaaaag aaatgtcaga agtaagagtt agagagaatg aaacattaga
     5701 cagcgcttta agaagattca agcgtcaatg tgcaatgtct ggcatcatgt cagaagtgag
     5761 aaaaagagag cactatgata agccaagcgt taagcgtaag aaaaaagctg aagcagcaag
     5821 aagaaaaaac gctaagaaat agtagtaaat aagaataaag aggtgaaggg gatgtccctt
     5881 aagcaaaagt tacaagagga tttgaaatct tcgatgaaaa acaaagatac agtgagaaaa
     5941 tctgtagtaa ctttaataag agcttctata aagcagtatg aggttgataa tagggtcgag
     6001 cttgatgaag atggaattat cgatgtaata gcaaaacaat taaagcaacg tagagatgca
     6061 ttagttgaat ttgaaaaagc tggaagagaa gacttaataa aagaaacaga aggtgaaatt
     6121 gaagttttaa aagagtacct acctcaacaa ttaagcgaag aagagttaga agaaattgta
     6181 aaatctacta tatctgaagt aggggcaact tctatgaagg atatgggaaa aataatgtca
     6241 gttatccaac caaaagttaa aggaagagct gacggaaaac ttataaataa gttggtaaaa
     6301 caaaacttac aatagattga tatagcctag agagaaatct ctaggctttt tataattttt
     6361 gggaataacg agtcatattt gagcatatct ataatcaaag aaaattttga aagaagggga
     6421 aaaatttgat tatatcagta aaagagatta ggaatttttt gataaacata tttataccac
     6481 taataatagg ttacttaagt agcattttaa caatgattat cagtggaaca gatatttcaa
     6541 cctattattt acagctcaca aagccaagct ttgcaccacc agcttttata tttcctatag
     6601 tttggactat attgtacata ttgatgggta tatcttcata cctaatatta agaaagggtt
     6661 acaacttacc taaagtaaga gatgcaattt tttattatgg attacaactt gtattaaact
     6721 ttatttggag tatactattt tttggatttg gacttagatt tacagctttg atagatataa
     6781 taataatgat tttggtttct ataataatga tttcgaagtt ttctaaaata gataagagat
     6841 ctggatacat aaatttggtt tatttaatgt ggcttgttta tgcaggattt ttgaactatt
     6901 ttatatggtt tattaataaa tagaatgtaa agattttgta aattaccctg tacttttaat
     6961 aatatcataa caaacgaaaa ataaatagac tattaaaaat aatcattttc atatacaata
     7021 atattaattt aaatataata aatttataaa aattgtattt aaagattaac atattaaagt
     7081 attagaattg gaaaggcttg aagggagagg tttttatgca aataatagta tctgggagac
     7141 aaatgaaatt aactgacgga ataaaaggat acgtagatgg aaaattaagt aggttagaaa
     7201 aataccttga tcctgaatca gaagtgaaag taacagtaag tgcgaaaaag gatagacaaa
     7261 aagtggaagt tacaataatt cctatcaatg gtcaaataat aagagcagaa gacgtagaag
     7321 atgacttata tgcagcaatt gatattgttt gtgacaaatt aagtagacaa gttgtcaaat
     7381 ataaaactaa agttaaagat aaagttcaaa ataataagag cataagattt gaaaatgttg
     7441 actttataga taatagcagt gaatttgatg attatgatga tgaagaatat gaaaatatag
     7501 ttatagaaag aagaaagaaa tttaatgtaa aacctatgag ttctgaagag gctatacttc
     7561 aaatggaact agtaggacac aatttctata tgtttagaaa tcaagataat tttgaaataa
     7621 acatagtgta taaacgtaaa gctggtggat atggtttaat agaacaagac taataaaaaa
     7681 ttgtatacgt ttagatttta tataagaaaa aataaagagg taatcatgtt gattttttat
     7741 caatatgatt acctttttat tgttcaaata atatattaga tattaggtgt ttattatata
     7801 aaaattttaa aactaatctg ttacaacatc aataaagtgc ataaatacct atttcaagca
     7861 taaaattaat taagggggta atgcttatgt tagaaatatc cactgattta caagtaaatc
     7921 aaccagttat tactgttaca agtaatacgt ttattagtat agaaaattat ctatcaatat
     7981 tagaatatga agttgattta ataagaataa agactaaggt taaaacaatt aaaatatctg
     8041 gggataaact ttcacttaaa tatattactg atagtgagat aggtataaaa gggattatct
     8101 acaatgttga gtatgttgat taggaggaga tacacttgat aagttttata aggggatact
     8161 atgttatagt tgtggaagga gtaggactcg aacaattttt aaatcatctt ataagaaatg
     8221 gtataagtgt ttacaatgta actagaataa aaaatactaa aatggagttt catatagata
     8281 gacaagatat aaaagaattt aagaatgttt atagaggtag taagtttgat ataaaggtaa
     8341 agcaaaaaac tggggttcct tttattatca aaagagttta taagcacaaa ggtatgtgga
     8401 tttgtgctct tgtatcgtta tttttattga tgtcgacatc acagtttgtg acagatgtat
     8461 atatacaatc tcctgaaggt ataaaaaaag aagctcttag aagtgagctt tataaggttg
     8521 gagtaagacc aggagtctat aagaagagta ttgatagaaa agaagttaga gaccatatga
     8581 tgtcgaaatt taatgatgtg gcatatttat ctatcaatgt gaaaggaaca aatatatttg
     8641 ttactgttac aaaaaaggca gaatccctaa aatctacaga ccaatcaaat tattgtaatg
     8701 taattgcttt aaaaaatggt ataatagaaa aggtaatccc tagaagtggt aaatcagtta
     8761 ttaaatctgg agatatagtc caaaagggag atgtcttgtt aaatggagct aataccaagt
     8821 caataccaga agtatgggca agtacttttt atgagtccac aaaaaaggca agttatgtag
     8881 atactgtaaa taaaaaaact ggagaaaaga aaaatatata tacactaagt ttttatgata
     8941 aagagtttac aatgagaaaa aatataaaat ataaagatta tgttgttgaa aataaagaaa
     9001 aaaaactatc aataggaaac tatacttttc caataaagat aaaaacgagc actttttatg
     9061 aaacaaaaaa ggttgaagta aaaaggaata aagaagagtt aaaaaaagaa ttatctgaaa
     9121 aagctttaaa agagttagaa tacataattc cagcttcggc tagaataata gatgtaaaac
     9181 ataactttaa ggttaataaa aatatgttag aatatttaat aaccgttcaa actagtgaaa
     9241 atattgctaa aatttatcct cttagtaaat cagaagcaga aaggtttata aaggaagaaa
     9301 gtaaaccaga tgaaggtgaa gaggaaattc cttccaatcc agaaaaaaga ccattaaatg
     9361 atataagaaa tgagtttgat gaggataata aggataacaa agaacaaaac aattctgatg
     9421 aaaataatag taatcaaaat agtaatagca ataacaacta ataaaattaa acttaaaatc
     9481 ttaggaggaa taattcaatt gataatacaa aagaaattta caatagcaga tggtaacttt
     9541 gaaagagagt tatttggcaa ctttgatgaa aatgtaaaat taatagagaa gactttaaat
     9601 atagatgtaa tacttagaga aggtaatata attttaattg gagaagaaaa aaatgtagat
     9661 tctgcattaa agcttatgaa tgaattacac cagactgtat caaatggtaa gcatttagat
     9721 aaacagagta tttcttattc attatctctt ttacttgaag gtagtgaaca aaaaataaaa
     9781 gaacttgagg ggacaatcgt tataactcaa agaggtaaag ctgttcaacc taaaacttta
     9841 ggtcaaaaag aatatataaa acttatagaa aataatgata taacttttgg cgttggacct
     9901 gctggaacag gaaagactta tcttgctgtt gctatggcag taaaagcttt taaaagagat
     9961 gaagtaagta gaattatact tacaagacca gctgtggaag caggagagag tctaggtttt
    10021 ttaccaggag atttaaagga caaagttgac ccatacttaa gaccactata tgatgcctta
    10081 tttgaaatgc tgggtgctga taaattcaat aagtatctag aaagaggtac tattgaggtt
    10141 gcaccacttg catttatgag aggtagaaca cttgataatt catttataat attagatgaa
    10201 gctcaaaata ccacttctga acaaatgaaa atgtttttaa ctagactagg atttggttct
    10261 aaagctgtag ttacaggtga tgtaactcaa acagatttac cacaaaataa gaagagtggt
    10321 ctaatacaag ctacagagat tcttaaaggt gttccaggta ttgggtctat aatgctaaca
    10381 gatagagatg ttgtaagaca tgagctggta cagagaataa taagagctta tgagaagcac
    10441 gacaaaagag aagaatttaa aaaagaagag cgtaaaaaag cgaaaatgca ggaaaagact
    10501 ttcaaaagaa agtaaaagtg tagtaatata gatatatagc attattaatg aataaaaagt
    10561 aggtgtagtg aatggatttg atactagatg atagacaaga taaattagaa gtaagtgagg
    10621 agttacttga aaagataaag gatataatcc tagaatgttt agattatgaa ggatatgatg
    10681 ataattatga agtgagtctt tcttttgtag ataataaaga gattcatgaa ttaaatagag
    10741 aatatagagg agttgataga gtaactgatg ttttatcatt tcctctttta agtgatgatt
    10801 ttgaagatgt tgagttagaa gaagaatcat taggcgatat agtagtatct cttgaaagag
    10861 ccttagaaca gagtatagag tataaccata gttttgaaag ggaagtatgt tttttaatat
    10921 gtcatagtat gtttcatctt ttaggatatg accacgatac agatgaaaat acaaaagaaa
    10981 tgagagaaaa agaagaacac atattaaata agttaaacat aacaagggag tagtttttat
    11041 gaaaccagaa aaaactcgtc aaggtataat taaagcgttt aatgcagcta tagaagggat
    11101 attgtataca tttaaatttg aaagaaatat gaaaatacat tatttaggtt cagttgctgt
    11161 tcttataata agtttgtttt ttaacttttc aaaattagaa atgataatgt tgcttatgtc
    11221 catatgttta gttgtagttg cggagatgtt taatacagct atagaaaaag ctgttgattt
    11281 agtaactgat gaatatcatg ttcttgctaa gatagcaaaa gatgttgcag ctggaggtgt
    11341 tctagtagca gcactaaatt cagtagtagt tggatatatt ttgttttatg ataaattgac
    11401 agatatatct gggatattaa tttataaaat aagagaatca gaattacata taaccttgat
    11461 atgtatatta ttggttctta tagctgttgt agttgtaaaa gcattgactt ctacaggtac
    11521 tcccttaaaa ggaggaatgc caagtggtca tgctgcatta gcatttgcta tagcaactgc
    11581 aattacactt atgactgaaa gggttgttgc atctactctt gcatatataa tggctgtgtt
    11641 agttgcccaa agtagaattg aaggaaaaat acataccttt tgggagacta tcgctggagc
    11701 tttattaggt gtgttgatag ctatattagt atttcagctt ggaatgttct ataattaaaa
    11761 tgaatgtaca taaaatattt aacataaaac aaatctgtgc taattaaatt tttatgatat
    11821 aatgtagagt attaaataaa aataactttt aattattgta tacaggagga gttgttgtgg
    11881 ataacataga attgttaagg ttggcagaag atgccagaca acattcttat gctccttact
    11941 caagctttag agtaggtgca gctcttttaa ctaagagtgg taaagtttat actggatgta
    12001 atatagaatg tgcatcatta ggtggaacaa attgtgcaga aagaacagct atttttaaag
    12061 cgatatcaga aggtgataag gatatctata aaatagccat agcaagtgat aactcagaaa
    12121 ataatgagca aacatatcca tgtggaatat gcaggcaggt aattatagag tttggttctg
    12181 atataaaaat aataacagga tatactaaag gagaggtgtt tgaacatgat ataaaagatt
    12241 tgttgccaaa ctatttctct ggaaatgatt taaaataaat taaaaattat aaataaattt
    12301 aagtaattaa aacaagaaga taaaaaagtt aggagacagt taatgttcaa atcaggtttt
    12361 gtcagtatag tgggtagacc aaatgtagga aaatctactt taatgaataa tgtagttgga
    12421 gaaaaaatag ctataatgag tgataaacct caaactacaa gaaatacaat acaagcagta
    12481 tatacagatg aagagatgca gatagtgttt ttagatacac cgggtattca caaaccaaaa
    12541 aataaattag gtgaatttat ggtaaaggcg gcaacagaag cctttaaaaa tgtagattta
    12601 atattatttg tagttgatga ttctaaaaaa ataggtcctg gagatagaaa gataatagag
    12661 gatttaagaa gtgttaaaac accaataatt ttagttgtaa acaaaataga ccaactagac
    12721 caaaaagatg agctatttga tataatcaaa atgtatgata gagaaggtat ttttaaagaa
    12781 atagtaccaa tatcagcatt aaaaggaaag aatacggata ccctaataaa agttatacaa
    12841 aattatttgg aagaaggacc aaagtacttt ccagattata tgattacaga ccagccagaa
    12901 agagtattaa tagcagaact tataagggaa aaagttcttc actatcttaa tgatgaaata
    12961 cctcatggtg tagctgttga aattgaaaaa atgaaagcga gaaatgataa ggaaatagta
    13021 gatgtatctg ctgtaatata ttgtgaaaga gattcacata aggggataat aataggaaaa
    13081 aatggtagga aactaaaagg aataggtaaa tcagcaagac aagatataga attactttta
    13141 ggttcacaaa taaatcttca attatgggtt aaagtcaaag aaaattggag aaatttacaa
    13201 aattatataa ataattttgg atataatgat aaataaaggt gggttatatg gataggaaac
    13261 tggatgcttc ccaagattta ttatatgaag taaaatcatt aattgataat aataaagtat
    13321 tggagttaag agaattaata gaggaatatc atataattga tatattcgat ataatggaaa
    13381 atttagagga agacatgaaa attcaattat ttgaagtact tcctttagat atggcatctt
    13441 ctatattaga agaaggtagt gtagagtttt ttattagtat tttatctaaa ctggatgtag
    13501 aacactcgaa aaatatactt gaacttatgt ctcttggaga tatggcagac aagttgagtg
    13561 aacttgaaga agaacaaaga gaacacataa taaatctttt gaatcaagag aatgccgatt
    13621 atgtaaaaga gttactcttc tatgatgaag attctgctgg tggaacaatg accactggat
    13681 atatttctat aaataaagat atgacagctc ttgaagctat agaccatatg agggaagaag
    13741 ctgaagaagc tgaaactatt tattatatat atgtggttga tgatgaagaa aaactagttg
    13801 gagtattatc attaagagaa ttaatagtag ctagagatgc aaatatagta gaagatttga
    13861 tgagtgaaaa tataatttct gtttatgtag atgaagatag agaagaagca gtaagattgg
    13921 tatctaaata caatttaata gcaattccag tagtagatag acaagaaaaa ttaaaaggga
    13981 taataacagt agatgatata attgatgtaa tggaagaaga agcaactgaa gatatgtata
    14041 aatttgcagg aagttcagaa catgaaagag aagttgctga aaaagaaaac cctacattga
    14101 aagagcaaat aatatcagca cttagaggaa gattaccatg gttaattatt accttagttg
    14161 gagggttgtt ggcttcgtta atactttcta acttagacta tataatgaac cctgtatatg
    14221 ctccattagt atttttcata cctgtagtaa ttggcatggg tggaaatatt ggaactcaat
    14281 catcatctgt tacagtaata acattatcta ataaggattt aaattttagc aatgtggtta
    14341 gagaaggtat tgtaggaatt attacaggtc ttttgtgtag tataataaca ggtattgtaa
    14401 tttactttgt tatgagaaag ttagacattg tattaattgt ttcaatatca ttatttataa
    14461 atatggtttt gggtgcaaca ataggtgcgt ttatgcctgt tttattaaaa aaaatggatg
    14521 cagacccatc tacagtttca tcaccaatta tatctacagc tcttgatata actggcatag
    14581 cagtttactt tataataaca acagcattat tgtcaaaaat tgtttaataa ctttatgtat
    14641 gatttatcct cttcttacta aaaatataag tataaatgta agaagggggt tttttaaaaa
    14701 tgaacaatat cagttgggaa gtttttaaaa aaacaggtag tatagatgca tatttatatt
    14761 tctgtgattg caaaaacata catgaagaaa ttgaagaagt aagggagaaa aaagaagacg
    14821 atgataatcc tgaacaccca ggggatagta cttaaggcta taaggtataa ggaaagtgac
    14881 ataatactta cactatttac aagaaagctt ggaaaagtat ctgcaatagc aaaaggagct
    14941 aaaaaaaata agagctcttt gctttcatca tcacagttat tttcttatag caattttacc
    15001 ttaaaaaaac aaggtaatat gtacaaggtt actcaaagtg aaataataaa aagtttttat
    15061 aatatatcct atgatataga agcattttct tatgctacat atattacaaa attagttgaa
    15121 aattctatac ttgaaaatca gactaataat aggctattta tattacttgc acaaactctg
    15181 tatttgtaca cacaggataa tacagataat aggtttataa cagcagcatt cgagttaaaa
    15241 tttttagact atataggatt taaacctata gtaaataaat gcattaattg tgaatgtaaa
    15301 attttacaaa attctgtgtt taatatctat gaaggtggta tactatgtag taagtgtagt
    15361 aaactttttg ataataatat aaaattagac ttaacaactg taagtcttat ggaatatgta
    15421 ttaagaaatg atattttaac atgtagtaaa gctaaagtat caaaatatat aacacatgag
    15481 cttgagaata ttctagacaa gtatttgaaa gtttatgttg ataatattaa ttttaaatca
    15541 ttacacgtat tgcaaagtgt aaaaaataat aagggagtgg ataatgatga gtagtaatat
    15601 aactattgaa atggttgatt cagtctttga aagagttcca ggagcaactt atgcagaagc
    15661 aaaagaggca ttagtaatgt gcaatggaga tgtaattgag gcagtaatat atttagaaaa
    15721 taaaaaaaat acttgtaatt gtaaagcaaa atctgctaaa gaaactatgg aagaagtatt
    15781 tggtaaagat ggagaaaata taaagttcca attaaaagag cttcttagaa aaagtagtgt
    15841 agtgagaata atagttgaaa aagatggaaa aactatgatg aacattcctt taacagttgg
    15901 agttgttgga ttagcttttg taccattagc tactttagtt ggattatcag ctgctgttat
    15961 aagtaaatat cgtataaaag ttcaaaatga agaagatgga gaaattgtag acttaggtga
    16021 gttgaatgaa gaaaaacttc atgttttgaa gaatatgata actaatgcag caaaagatgt
    16081 aaaggatgta gtagttgaca aaaaagaaga tgataaggat gtaactgctg accttatgaa
    16141 agaagaatct gaaaagaata aagaagacga agacgaaaat aaatagatat aaagtgactt
    16201 ttaaaaaatg aagagaagat aattataaga attatcgcta acattgacaa attttataga
    16261 atttgatatt ctaaaagttg tagaagaact attaaaatag cctttcgtgt agacgagagg
    16321 ctttattgtt ataggaggga tttttatgaa ttttcaagag atgatactgg cgttgcaaaa
    16381 atactggtca aagcaaggtt gtatcatgat gcaaccctat gatattgaaa aaggtgcagg
    16441 aactatgaat cctaatactt ttttaagatc attaggacct gaaccttggc aagtatgcta
    16501 tgtagagcct tcaagaagac cagcagatgg aagatatggt gaaaacccaa acagactata
    16561 tcaacatcat cagttccaag ttatactaaa accttctcca gataatatac aagagttgta
    16621 tttggagagc ttaaaagaaa ttgggattga cccaagtgag catgatataa gatttgttga
    16681 agataactgg gaagctgcta cagttggtgc ctggggactt ggatgggaag tttggctaga
    16741 tggtatggaa ataactcaat ttacgtattt tcaacaagta ggtaatattg aatgtgagct
    16801 tgagacagga gaaattacat atggattaga aagattagct atgtacattc aagaagttga
    16861 tagtgtttat gacttaaaat ggaatgataa aataacttat ggtgaagtat ttaataaagc
    16921 tgagtatgaa aactcaatgt atgcatttga attatgtgat gcagatatgt tgtttaatct
    16981 gtttgatata tatgaaaaag aagcattaag acttatggaa aatggacttg taataccttc
    17041 atatgactat gtgttaaaat gctctcatgc atttaataca ttggatgcaa gaggtgcaat
    17101 aggagttagt caaagagctt catttatagg tagagttaga aatatggcaa agactgttgc
    17161 agaaactttt gtaaaacaaa gagaagaaat gggattccct cttttaaagg acggtgacaa
    17221 ataatgaaaa attatctttt atttgaaatc ggtgttgaag aaatgccatc aagatttgta
    17281 ggcagcacac tagaacaatt aaaaaacaat cttagcaaat tatttgatga aaatagaata
    17341 gcttttgata aaataaatgc gtatgggaca ccaagaagac ttactttagt agtcgaagga
    17401 ttgagtgata gacaaagtga tttagaagag gaaataaagg gtcctgctaa gaaaattgct
    17461 gttgatgcag aaaataattt aacaaaacca gcattaggat ttataaaaag caaaggatta
    17521 agtgaatctg atatatactt taaacaagtt ggaaaagatg aatatatctt tgctactata
    17581 aagcaagatg gaaaagaaac ttcagaagta ctaaaagata tattaccaga tactataaaa
    17641 tctgtagtat tcccaaaagc tatgcgttgg ggaggaaaaa atcttaagtt tgcaagacct
    17701 ataagatgga tagttacatt gttaaatgat aaagtattag agtttgattt agaaggaata
    17761 gtatcttcta atgtaacaaa aggtcataga tttctaggtg aaagtacaat agaagttgat
    17821 tctatagaag actattttga aaaattagaa aaaaattata tagttttaga ccaaaataag
    17881 agaaaagaaa tgataagaaa acaatgtata gaagtggcaa attctttagg tggagaagtt
    17941 gaatttgatg aagatttact tgaagaggta acttacttag ttgaatatcc aactgctttt
    18001 tatggagaat ttgatgtaga ttatataaaa ctaccaaaag aagttgttat aactcctatg
    18061 aaacaacatc aaagatattt tccagtactt aaagaaggaa aactgttacc taactttata
    18121 gctgttagaa atggtgattc ttatagaata gacaacgtta aagctggaaa cgaaaaagtt
    18181 ttagaagcaa gacttgcaga tgctttattt ttctatagag aagatactaa gagaaactta
    18241 gaaagctata tagaaaaatt aaaaactgtt gtattccaag taaagctagg aactatttat
    18301 gacaaaactt taagacttga aaaattaagt gctgatatac ttgacagttt aggtttagta
    18361 gatgaaaagg cagatactat aagagctgct aaattatcta aggcagattt agttactgga
    18421 atggtaggag aatttgatga attacaaggt tttatgggaa aagaatacgc aaaagttgga
    18481 ggagagaacg atgttgtaag tgaagctatt tttgagcatt atcttccaag atttgcagga
    18541 gatatacttc ctaagactaa cgcaggggtt gccttatcaa tagctgataa gttggattca
    18601 atagtaggtt tctttgctat aggaatacaa ccaacaggtt ctcaagaccc atatgcttta
    18661 agaagacaag ctttaggtat acttagtata cttatggaca gaaaagttgc tgtggatata
    18721 aaagtactta ttgaacatgc tttagataat tattctgaat tagattttaa tagagaagaa
    18781 gttgttgaac aaataatgaa tttcttcaat gagagagtaa aaaatctgtt taaagattta
    18841 ggaataagat atgatgttgt cgatgctgtt ttaagttctg atataaatga tgtttctgac
    18901 atgcatatga gagctttaga gcttaataat tggctagaaa aagatgaatt agtggagatg
    18961 cttactgcat ttaacagagt ttctacttta gctcaaaaag cagaaagcga taaaattgat
    19021 gaatctttat taaaagaaga tgctgaaatt aagctttaca atgaatttaa acaagtaaag
    19081 gtaagagtta agcaattatt aaaagataag aaatatggtg ttgctttaga tgaatttgca
    19141 tctttaagac cagtagttga taatttattt gatacagtta tggtaatgga taatgatgaa
    19201 gctataaaga acaatagact tgctttactt aaacaagtat acgatactat gctagaaatt
    19261 tgtgatttat ctaaaattgt ttataaataa aattcaagct atttataaat aatgaggcta
    19321 tctcaaaata gagataagcc tcttttttat ttttgaaatg ataaaaatat atttaattgc
    19381 atacaaaaaa gagtacctca tgaactgaaa agttaatttt gaggcactgc tactttttat
    19441 aagtaaatgt aataaatcat attttatact tatcagttat tttgtttaag atgtcatcat
    19501 ttgaatttac ccattcataa aaatttcctg ttgaagcgtc agatatttta taaagcattt
    19561 caggaatatc gctagcttta aaatctccaa gtgattttcc taatctagtc ttgcctattt
    19621 caaagctacc tccaacaaat aattcaaatg catctgtaga tacaccatca acattcttct
    19681 ttttaccaca taaacctata ctaccaactt ggtgaacacc acatgaattt aaacatcctg
    19741 atatatataa cttaggtgct ttatttaaaa tttcttcatt attctgtaat ctaaaataat
    19801 ctatgatttc atgtaacatt ttctgactat tttgtatacc catttggcaa attggaacac
    19861 ctatgcaaga tatgctttgt tctaattgag aattacaact aatagtttta cttatttcta
    19921 atattctttt tgcttcattt ccatttaaat ttaaaatata taatccctct gtcattccta
    19981 gtctaatcat agggttttca atattatcta attctttaag taaagtattt aaatcctttg
    20041 ttaacaaaat acctccaact ggatgtatat acacagtgta taaaccatct tgtttttgag
    20101 gtattaatct ggaatcttgt atacatattt taactccaga tttagagtag tctatttctt
    20161 caatattgag ttcaagacca ccattttctt tttctaattt atagtattct ttaaattttt
    20221 ttaagaatag ctcttcacca agtgattcga ccatatatct aactctagct ctgtttttat
    20281 tttcataatt gccataatcc ataaagaatt taatcatacc ttcaacacaa tataatgcat
    20341 ctttcggctt tataagttcg tcaagctcta aagcaacttt gggttgttta cctagacctc
    20401 cacctaagaa aactctgaaa tattgttcat catttttcaa agtagccaca aatcctaagt
    20461 cttgtatact acaatgagca gtatctgtgt aacaattaga ataagaaact tttaattttc
    20521 taggtagatg gtatgtagta gcatttttga taaaatattt atcagtagca actgcatatg
    20581 gagtaacatc aaaggcttca ctagggtcaa ctccagacaa tggagataat ccaacatttc
    20641 taggataatt accaccacca cctctagtga atatatcgtt ttttattcct tctttcatga
    20701 tatcgacaat cgaatctata gataaatcgt gtaattgtat agcttgtcta gttgttaagt
    20761 gtatcttatc taagttgtgt ttaatagcca tttcatagat tttattgagt tgatgatgag
    20821 ataaaactcc agcagatact ctaagtctta tcataaaaga ttttttgtct ctttgagcat
    20881 aaacaccgta tccaccagaa aatcccttat attgcatttt atttatttct ccatttaaaa
    20941 attttgaaga atattcttta aaattaggta tttcatttaa caatattttt ttaaaattgt
    21001 ccattatttc acctcattat tagtcttgta taaaaatata tctattgtat aatagatata
    21061 tatattatta tctagtcagg ataaaaatat aacagaataa ttctattaaa agtaagaata
    21121 ataaggacat agtgagtatt ttgtttttat tttacaaatt aagtataata tttttgtttt
    21181 ataaaatata attaagataa atagataatt ttgcaatttt aatatataat gtttagtaga
    21241 gtgtgggagt agcacttaat atttgcagaa ggagtttttg tatatgggag aaataataaa
    21301 tgcattagat agagccctag acataatttt gctgttatat catgaaaaga gagaaatggg
    21361 aatcactgaa ataagcaagg ctatgggagt atataaaagt actgtacata gaactttggt
    21421 cactcttgaa aataaaggtt ttgttataca aaatgcagaa aatggtaagt attggctagg
    21481 aataaattta tatgcaatag gtatggttgt gggagaaaaa atgtcattga ctgaaatcgt
    21541 aaaaccatat acgaaaaaat taaatcaaga atttaacgaa gtagttaatg tatctatatt
    21601 agaagaaaga gctcaagatt ctccaagaag tattataata cataaagagt atggttcaaa
    21661 tcaattactt tcagtaaatc catccgttgg ctcaagtagt gaatgttatt gttcggcagt
    21721 gggtaaatgt cttatggcat ttaatgattc tatagatttt gaaaaatata gaaaaacacc
    21781 aatacacaag tatacagaac atacaattga taattgggat gatatgatgc tttttttagc
    21841 aaaaataaaa gaacaaggat atgctataga tgatgaagaa ctagaacatg gactaacttg
    21901 tattggagca cctatcttag ataaaaacaa taaagctatt gcagcaataa gtttgtcagg
    21961 tccaactata agaatgagag aaggggactt tgagtataag attaaaagag ttatagaaac
    22021 tgcaaaaagt atatctgaac tttttagatg attttttaat ctagataaga aaaagaggtt
    22081 atatttaaat tgttatttgt aaatatcagt ttaaatatag cctctttttc tttaatctaa
    22141 aattaaatat tcttaaaatt taaaatgtta ttgaaatata taagcgaatg tgttatcttt
    22201 taattaagca ataaaaatag aacaaggttc tatactatag aacaaaaatt aatgcatata
    22261 tagtatttat ataaaatata aatattatat atttaaaatt aagtaaaaat attacataaa
    22321 ggggagattt gagaaatgga aaaagtcaag ttgagtgttg aaagatgtaa gggatgctat
    22381 ctttgtattg aagcttgtaa aaaagatgca ataagtcttt ctgactacac aaataaaaaa
    22441 ggctttgtaa ctattaaagt cgatgaagaa aaatgtattc aatgtggcgc ttgttataca
    22501 atgtgccctg acttagtatt tgagatatta taaattattt ttattagagg ggagaaaagg
    22561 tatgcaaagt ttattaatta aaggaaatga agctttagca gaaggtgcta tacgtggtgg
    22621 ctgtagattt tatgcaggtt atccaataac accacaatca gaaatattag aaatgatggc
    22681 atggagacaa aaagaagttg gaggagtatt tatccaaggt gaatcagaaa tagccagtgt
    22741 aaatatggtt atgggagcaa gtgcacttgg agcaagagct atgacttctt catctggtcc
    22801 agggttttca ttaaaacaag aaggtattgc atattgggta tcagctggtt tgccagcagt
    22861 tgtagcatgt gtacaaagat atggtatagg tgatggattt ataggagctg gtcaagataa
    22921 ttattggcaa gcagtaaaag gtggcggaaa tggagattat catttaatcg tgttggcacc
    22981 taatagtgtt caagaaaata tggatatggc atatgaatct tttgaactag ctgaaaaata
    23041 tatgcatcca gtacttatac tttctgatgc tacaataggt caaatggtag aaccttgtat
    23101 agcaccacca atgaaagaat acgatatgga taaagctcca tgggctgcaa aaggaacacc
    23161 tgtaggagaa aaaaataaag taataactga tataacatac tttgaagacc aagatacatg
    23221 gcaaaaaggg tatatagaaa aaatgagaaa agttcaagaa gatgaacaaa gatgggaaga
    23281 aatagaaata gaagatgctg aagtggtgtt tgtggcatat ggtatttcat caagaatagc
    23341 agaaggtgca gttagagatg ctagagcaaa aggaatgaaa ttaggactaa taagaccaaa
    23401 aacattgtgg ccttacccaa gaaaggcatt taagaaatta agtgattcat tgaagggact
    23461 tgtaacagtt gaaatgaaca tgatggcaca gatgaaggaa gatgtaatta tagcatgtga
    23521 taatagattt ccagtatatt cagtagcaac agctcaatat atggcagaag taaatcaatt
    23581 aatagatttt gctcaacaga ttatagatgg aaaagctaag caagaggagg tattttaatt
    23641 atggctaaaa aagcaccagc aataatgatg tgtgaaaata tgttctgtgg aggctgtgga
    23701 cactctatag tcgatagaat tattggagaa gtattaacag aaatggatat atgtcaagat
    23761 acaattatat gttcagatat aggttgcaac catatgttcc aatttagtat gaatgtagat
    23821 gttgtcgttc cacctcatgg taaaatggga gcagcaatgg cagctatgaa aagagtcaga
    23881 ccagataaat atatatttac aatagctgga gatggtggag cttatgcaat agggcttgga
    23941 gaaactatgt cagctgctac tagaaatgat aatgtaatat ttcttgttat gaacaataac
    24001 atgtttgcaa tgacaggggg acaaatggct ccaactactg tagaaggtca atatacagct
    24061 tctactccag cgggtagaaa ttacgcagat catggttcaa catttgatgt tgtaaaagtt
    24121 atgggaaatt tagatatagc atatctagcg agaggaacaa taactaaccc taaagaaata
    24181 aatacaacaa aaagatacat caaaaaagct ctagaaaaac aaaaagaaaa gaaaggtttt
    24241 tgcttaatag aagtgcttgt accatgtcca acaaactggg gattaaagcc agtagatgct
    24301 atgaagagaa ttgaaaatga acttgttcca aagtatccac ttggagaatt tattgattag
    24361 ggaggaaata atatgttgaa tgaaattata tgtgctggtt ttggaggaca gggagttctt
    24421 acagcaggaa aaattataat gtatgttgca tataaggcag aaaagaaaat tacatggttc
    24481 ccatcttatg gaaatgagat gaggggagga agtgcaaatt gtaatgttgt tgtaagtgat
    24541 gaaaaaattg caaatccttg tgtaaaaaat ccagatatat tattgggaat gaacagcgta
    24601 tcagtagata agtatgaaag tttaatgaaa aaaaatggtt atatgtttgt caatgaatca
    24661 ctagtaagta aggatagaaa atacagagag gatattaatg ttataagagt acctgttact
    24721 gagatagcac aaaatttagg taatgaaaaa gctgctaata tttgtatgtt aggtgctatg
    24781 gtaaagaatt tggatttatt tacaaaagaa gaatttgaaa atggtatgtg tgaatacttt
    24841 gaaaatcaag gcaaaggtaa atttaattct aaaaatgtag aagcatttga agctggatac
    24901 gactacgagt gaatatttta aatatttaaa ataattattg aaatacttat ttttatatgt
    24961 tagtatttaa ttaataaaat atagaacgtc gttctggaca ataggacaaa atctttgtgc
    25021 ttgaatataa gaacgacgtt ctatgtaata gaattaggag gtgttaaatt gaaaatttta
    25081 gtaataaatc ctggcggtac atccacaaag atttctgttt tcaaagatga aaatgaaatt
    25141 ttgaaaaaaa atataaccca tacaagagaa gatttaaaaa atttttctaa agtatttgat
    25201 gaatatgatt atagaaaaca actgatagta gatattttaa gtagtgagaa tcatagtata
    25261 aattcttttg atgcagtagt tggtcgtggt ggacttatga aggctataaa aggtggtact
    25321 tatactgtga gtgaagaaat gattgaggat atgagaaatg aaataaatgg ggaacatgca
    25381 tcaaatttag gtgcattatt agctaaaact atagctgatg aaataggggt acaatcattt
    25441 gtagtagacc cagtctctgt agatgaattt gaagatgtat caaggattac aggaatatca
    25501 gatatagaaa aagcaagctg gctacatgca ttaaatcaca aagctgtttc aagaaaaatt
    25561 gcacaaaaac taggtggaaa atacgaagat tataacttca ttgttgctca tttaggttca
    25621 ggaatatcga ttgtagcaca taaacaagga aaaatgattg atggaagtgg tggaaggtct
    25681 gatggtccat tttctccaga aagaagtggt ggattgcttg catatcctct tatagaattg
    25741 tgttactccg gtaaatatac tcatgatgaa atggtagcaa aagtaagttc gattggaggc
    25801 atgtacgact atttgggcac aaaggacatg atagaaattg aaaacagaat caagcaaggt
    25861 gatgaaaagg caaaacttat aatggatgca ttcatttatc aagtagcaaa agaaatttct
    25921 atgtatggag cttcactttc aggcaatgta gatagagtta ttttaacagg aggaatatct
    25981 cattcagaaa tggtagttaa aggagtcata gaaaaagttt catatatagc tccagtagag
    26041 gtagtaccag gggagatgga aatggaagca cttgctcttg gagctcttag agtacttaat
    26101 ggagaagaaa tagcaaaaac ttattaatat ttaggagaag gagtatgtta aaagatttta
    26161 ataatttatt ggaagaagct aaaaagcttg aacctgttat agtaagtgtt gctgttgcag
    26221 aagacaaaga agtattaaag gctgtgaaat cagcaacaga tttaaatatt ataaagccaa
    26281 ttctcgtagg agataaagat aaaatagaaa atatatcaaa agaaattgga ttaaaaggtc
    26341 atgaaataat aggatgtcaa aatcctgatg aatcagtcaa aaaagctgta gagatagtaa
    26401 aaaataagga agctaaagtt cttatgaagg gattagtaaa tacgagtgtg tatatgagag
    26461 ctatattaaa taaagaaact ggtctcagaa caggaagatt attgagttta cttgcagtat
    26521 atgaattacc tcaataccac aaacttcttt attgtactga tagtggaata aatgtatcac
    26581 caaatctaaa tcaaaaaaaa gatataatgg taaatgcact tcttactatg aaaagtatag
    26641 gcatggaaaa tcctaaaact gcgatactta ctgcaaatga aatggtagac cctaagatac
    26701 aatctacagt agatgcaaac ctacttgttg agatggaaaa atcgggagaa ataccaaatt
    26761 gtatagtgga aggtccaata gcatttgatg tagcatttga tgcacatgca gcagaacata
    26821 aaggtatcaa aagtaatatt gctggtgatg ttgatttact agtatttcca aatatagaag
    26881 cagggaatat attaggaaag tcatggctta gatttaataa agctaagtgg gcaggtatag
    26941 ttttaggagc aacaaatcca ataattttag gttcacgttc tgatacagct gaaattaaaa
    27001 taaattcaat agcattagcg tgcttagcat cacaataaga aatcattatt aaggaggata
    27061 aattattatg aaatatgcaa aaagaatgga tatggtaaaa gcttcagcta taagagattc
    27121 tcaaaagaaa atagctcaga aggtagcaag tggtggaacg gttatatctt ttgctgcagg
    27181 acttcctgac ccaaatttat ttccaataga agaaataagt gaagtaactc aaaaagtact
    27241 taaggaaaga ggtaagtttg ccctagcata tggacctact aaaggtgaag atgagttgtt
    27301 agagctatta gtaaaaagaa tgaaagaaga ggaaaatatt gatacaaaag cagaaaatat
    27361 tataataact actgggtcac aacaagggat agcattaagt gctatgattt tattagaaaa
    27421 gggagatgta gtagttacag aaaatccaag ttacttaggt gcaataaatg catttagacc
    27481 ttatgaatgt gattttttag gagtagatac agacgaagaa ggaatggtaa cagatgattt
    27541 agataaatta ttatctgaaa attctaacat aaaggtgata tatgtaatac caacatttca
    27601 aaatcctaca ggaaaaacat ggtctttaca aagaagaaaa gattttatgg aagttgtaag
    27661 taaatatgat gtcatagtaa tagaggataa tccttatgga gaaataagat ttacaccaga
    27721 acctctacct acacttaaat ctcttgatac taaagataaa gtattgtacc ttgggtcatt
    27781 ttcaaaggta ttatgtccag gatttagagt tgcttggatg tgtggtaatg tagacatcat
    27841 agataaggca gaacttttaa aacaaggtat agatttacag tcaaatcaat tttctcaagt
    27901 acaagtaata gaatttttaa agaaatacaa cttaaatgaa catatagaaa aaataagaga
    27961 agagtataaa aaaagatgct tactaatgtt ggatgctatg aagaaacact tcccagagga
    28021 agctaaatat acagagccag atggaggaat gtttatatgg gttgaacttc ctgaaagcat
    28081 aaatgtagat gatttattag ataaagccat tgatgctgga gttgcttatg tttctggaga
    28141 gtctttcttt gcaaataatg gtccaaaaaa tactatgaga cttaactata caacaatgtc
    28201 agaagaacaa attgttaaag gtgttgagat acttgcagaa gttataaaaa aagaactcaa
    28261 ttatgcttgt gaataaatat aaaaagtgag ggtttaaata tgggagaaaa agaaaacggt
    28321 ataaagtttg gaaagttaat gtttcaactc gttattatag caatatcttg ggaattagtt
    28381 tatattatac catttatcca atatacatta tatgatccaa tcctaaaagc attagaatgc
    28441 acaaatactc aactaggttt tttacttaca atttatgggt taggtaatat atttggagct
    28501 cctataggag gctggttagc agatagattt gattatagaa agataatttt aggttctgta
    28561 tttcttaatg gagtagtatc atttttgttt gcatttaata tgaactatcc atttgcagtt
    28621 gctacttggg tgggttgtgc aatcacatca ttagttatga actatccttc tatggtaaag
    28681 atacttagag ttataggaaa agataatcaa ggtaaggttt atggatttaa tgaagctatg
    28741 gttggagttt ctggcgtagt aatgggagca atattcttat atatttatac atgctttgca
    28801 actcctacat tgggtatgag atgggtaatg atatctttag gaatattatc aatagcaatg
    28861 tgtccagtat tatggtttgt aattaaagat gtagatatta aagaagaaaa agaagaagca
    28921 ggtgaaaaaa tgtctgctgg tgattttata acagtactaa aatcaccaaa tacttggcta
    28981 gtaggaataa gtatattctg tgtatattca ttcacagtta caatgtctta ttttacacca
    29041 tatataacat ctgtacttgg aggttctgta gcactatctg gtgcattagc aataataaga
    29101 caacatggtc taaaattgtt tggagcacca tttggaggat attgtgcaga caaattaaaa
    29161 tctcctacaa aagtactttt accaatatat gtatttggta tagcagtaat agttttattc
    29221 ctagtattac ctgcatcaac tccaatgact atattcatag ctctaacatt tgtagtaggt
    29281 atattaggtt acatgggaaa aggtatatat tatgcagtac aagatgaagt aaaagttcca
    29341 gttaaatatt cagcaactac tataggtata gctgcagctt taggattttc accagatgta
    29401 ttccaatttg cgttaatagg acactggata gatacttatg gcaataaagg ttacacatat
    29461 acatttatat tccagatagc aattttagtt attggaatac tatcttgttt atatatatta
    29521 aaggttaaaa agagaagagc aagtaaatta gaagcttaaa tattcaattt ggtgattaag
    29581 tacatgtaaa tacaatactt attttgatga atgtgattgt ataaataaca taaaaaatat
    29641 caatagatta agttttataa taatgtttag agaataaaat ataaaaatca aaatgttatc
    29701 aagttttcta agttttagca taaagttacc cctaaaggta taaaattgag tagtaattat
    29761 tatctatttt cttacttaca taaacctatt taatttaaat tctaagagaa tttcaaaata
    29821 gatggcagtt gtactcaatt ttatacctca taataaatac gaaaatttaa aatctaacta
    29881 gatactattt aattatatat taataaatat atattgtatt atttatataa tagtaatttc
    29941 aggcttttca ttatatgtat tccaatttat attgtttgat tattgttcaa gcacacatcg
    30001 ttaaaaattt tataaattta tgtttagata tgattaatac tcttgatttt agaaataaat
    30061 atttgtagat tgtagattaa aaataatata aataaataga aggtagattt tttagatata
    30121 ttaagatgaa gattatataa aatattgaaa tatagctgtt taaattattg tctataaata
    30181 taaaatgaat ggtggtgagt tacgtgaccg atttagttaa caccattaat ggaattgttt
    30241 ggtcacctgt attagttgca atgtgtttgt gtataggatt atatttttcg ttgaaattaa
    30301 aattttttca aataagagat gtaaaagaaa tgtttactct cttgcttgaa ggagatagtt
    30361 ctcatgttgg gatatcatca tttcaaggct ttgctacatc tatggctgga agaataggga
    30421 ctgggaatat agctggtgta gctgtagcaa tagcaatggg aggtccagga gcattagtat
    30481 ggatgtgtat aatggctaca ttaggttcag caactgcatt tgtagaatct actctagctc
    30541 aaatatataa agatgagcat gatgggcagt atagaggagg acctccatat tattttgaaa
    30601 aaggtcttgg gtggaaaaag tgttcagttt tattttcaat agttgcaata ataagttata
    30661 cattttttat gcctggtaca caatcaaata ctttatatct agcaatgaat caagcatttg
    30721 gattttcaaa aatgacaata gcagtcgcat cttctattgc attagcaata ataatttttg
    30781 gaggaattaa aaggattggt ttatttgctg aaaaagtagt acctataatg ggtggagcat
    30841 accttattat gacattagta ataattataa caaatattag tttagttcca gaagctattg
    30901 taacaatgtt taaatctgca tttggatttg gttcagtttt tggaggaatg ttaggttcag
    30961 cagtatcttg gggagtaaaa agaggtatat actcaaatga agcaggagaa ggttctggga
    31021 cttatggtgc agcagcggca gaagtaagtc atccagccaa acaaggatta gttcaagcat
    31081 tttctgttta tgtagataca ttattaatat gtatggctac aggtatcatg attgtaataa
    31141 caggaatgta taatgttaca ggtgtttgca caacactgcc aggtgtagaa cctgggccac
    31201 aatatgtaca gagtgcaata aatacattac atccacaatt aggtgatatt tttatagcaa
    31261 tagttttatt attgtttaca tttacaactt tattagcctt ttcatatatg atggagacta
    31321 atgtatctta cttagtaact agaattaaag cagaaaaata tgaaaagtac ataattaacg
    31381 tctgtagatt tatgcttata gcatgtgcag caatgtgttg ttttatagaa ccacttacaa
    31441 gttgggcatt aggtgatata tgtataggtg tattaacata tataaattta attgccatct
    31501 ttacactaaa aaaaccagct ataaaggcat attatgatta tataagacag aaaaagatgg
    31561 gaataaaaag gagcgataga acttttaatc ctatagaatt agatataaga aatgctgagt
    31621 tttgggaaaa tagatactta aaagagaata agcatgagtt aaattaaagt tataataaaa
    31681 tataagaatt actaaataaa cttagtggga ggtaagattg gtatgaaaga aagaataata
    31741 caagtggcgg attcaaagaa agaaaaaatt ctagggctat gtcaatcttt atatgatgaa
    31801 ccagaaattg ctttgcaaga atataaatct gctaaaaaaa tagcagaatt tctaagagaa
    31861 gagggctttg atgttgagga aaacttggca ggtatggcta cagcttttaa agcaaccaaa
    31921 aaaaatggag atggtcccaa aatagcattt atagcagaat atgatgctct tccaggaaat
    31981 ggccatgcat gtggacatca tttaattgca tctatgggag ttggagctgg aatagctctt
    32041 tcaagtatac tggatactta taagggagaa gtcagtataa ttggaacacc tgcggaagaa
    32101 actggagatg gaaagcctta cttgatagaa catggtgtat ttgatggcta tgatgcagca
    32161 atgatgatac atccaaattc gaaaacttgt gtgacaccag aaattatagc aataggagga
    32221 ttggacttta tatttactgg aaaagcatct catgcaggag ctaaaccata taatggaata
    32281 aatgcactag atgcagtagt actactatat aataatataa atgcactaag acaacaactt
    32341 atagatggaa cgagaataca tggtattata ctagaagcag gaactgctgc aaatgtaata
    32401 cctgatatgg gaaaagtaag attagagata agagcaaaag aacaaaatta ctttgatgaa
    32461 gtagtagaaa aagtgaaaaa ttgtgctaga ggtgctgcaa tagctactgg atgtgaactt
    32521 gaattttatc actttgaacc aacttgtcaa ggattaaatg aaaataaagt tttagtagag
    32581 atatttacaa aagtcatgga ggaatttgga atatatgaag atgaacaagt attgctaggt
    32641 tcaacagaca tgggaaattt aagtcaaata atgccatgta tacatccttt gatgaaattt
    32701 tctgaaaatg gagaagaatt gcatacgaaa gaatttttag aagcttccat aaatccatat
    32761 gcaaaagata gagtaattga tggtataaaa atattggctt tgacaggatt taatttattt
    32821 gaaaattcag agttattgaa aaaaatgaaa gaggagtaga ctttaaacaa aggggacttg
    32881 actatgacta ataaaactga aggtaaaaag aaaaaattaa cattcccaga tgcatatgtt
    32941 ttgatgatag gtcttattgt atttgcagcg ataatgactt atattgttcc ctctggaaca
    33001 tatagtagaa ttgaagattt aaatactgga aatatgcttg ttcaagctgg aagctttaaa
    33061 tatggagagc aacatccagt tagcataatg gatgtattta gagctatacc aagtggactt
    33121 ggaaaaaatt ctgtaacaat tttctttgta cttattattg gaggagcatt tggtatagta
    33181 aattttactg gttcactaga tgttttaatt ggcagagtta tatctaaaag tcaaggcagt
    33241 aaaaaaggtg agattataat tacaagttta gtattaactt ttggcttagc tggtggagtt
    33301 gtaggtatgt atgaagaatg tattgctttt ttaccaatat taatgactct gtgcattgct
    33361 ttaggatatg atgctttagt tgcagctggt attttattgt taggacttgc atcaggttat
    33421 ggttcagcta ctattaatcc atttacagta gggctagcac aaggtatttc tgggttacca
    33481 cttttttcag gcatgtggtt tagatggata ttttggattt gtactatgat tttactttca
    33541 ggatacataa ttttttattg taaaaagata aaaaaagacc cgagcaaaag cttagtttca
    33601 gatattgatt atagtcattg gcaattagat gagtcttcta taaaagaaaa attatcatct
    33661 ggtaacatca gagtattagt tacattcttt ggtgggatac ttgttttgat gataattatt
    33721 gtaaaatttt ctttaggtat gtcagattta tcagcttact tctttgcttt aggaatttta
    33781 tgtggggtag tttatggaat gagtccaaat aaaattgctt ctgcatttgt aaaaggtatg
    33841 caagagatgg tttatccagc cattctgata gggtttgcat ctagcatagt agtaatacta
    33901 gaacaaggag taattttgga ttcaatagta catggattat cattagtttt aaattatgtt
    33961 cctagaattt tcagtggtgg agtaatgatg attatgcaat ccataattaa tttatttata
    34021 ccttcaggca ctggtcaagc tatggtaacc atgccactta tggctccact tgcagatgtt
    34081 ataggtgtac caagacaagt tgcagtttta gcttatcaaa tgggggatgg ttttgcaaat
    34141 ggaatagttc caacacttgg agttcttatg gctggtatat ctattgctca tataccttac
    34201 acaagatggt ttaaatttgc atctaagata atacttatgg agttggtttt agggtttata
    34261 tttttaatga tttcagtaca gattaattta ggtccttttt aatgtattta cgtaatatgt
    34321 ttatagaatt ttaaggtaaa ttatattgag gttaaatatt ttgaattata atatgttagg
    34381 tttaattatg gtttttgaaa taaagggtga actttgtgaa gttcactctt ttaatttgta
    34441 gcacaaaata tcattaatga ttacagcttt ggtgataaaa ttaatttcaa gatatttata
    34501 ggaggggagt aatatggaga ctttaaaaat aagaaatatt gaattgagtg gtacaaatta
    34561 tgagatagga tatgaattag gtaaattaat tgccaatact ccaaagatac tagaaggaca
    34621 aattaataaa tctaatgtag taacaaagga agaagagaaa gagatgttag agttatttga
    34681 taaatattgt ccaggtctta acgaagaact gaaaggtttt gctgatgcag ttcaagtgaa
    34741 ttgtaatcaa attttgtatt atactatgac ctatcttaaa cccggttgca gtcaagttgc
    34801 gttagcacca gaattaacta caaatgggca tgtactattt gcacgtaatt tcgatttttc
    34861 acacaatatg gaggactttg ttttatgtaa aacgaaagta aaaggaaaat atgcacatat
    34921 aggcactact gttatgcaat ttggaagagg ggaaggaatg aatgaatgtg gattaggcgt
    34981 gagtcagtca tcatgtggta ttcctgtagg taattcagtt ggtttaagaa aaccagccgt
    35041 tgtaggatta cagttttggg ctgttattag atatttatta gagaattgca aagatgtaga
    35101 tgaggcgtta gaatatatag aagatatgcc aattgcatat aatataaatt tgttacttgc
    35161 agataagtct ggaaatattg cacttgtaga gactttagat ggaaaaaaag gagtaaatag
    35221 aattaataga ctagaaaagc agagggaaca ttttttacac tctactaacc atattcatat
    35281 tgatgaactt cataaattag aaccacaatc aattaaaaat tctattcata gatataaatt
    35341 aattaaagaa tatataaaca attctaaaaa agttggagag aaagagttaa tgaatttgtt
    35401 gtcctcaaag tatccaaatg gtttatcttg taattactat aatgattttt ttggtacttt
    35461 aaaaagtata gttatggatt taaatactgg taaatttaat attttatggg gtggattaga
    35521 gagtaataac tgggagagtt actatttaaa agacgacgtt aaagctacaa cacatctgat
    35581 aaatataaac attgaaaagg caccatctga ctttttcgat ttcgttgaat aaaaatgtgt
    35641 ttaattagtt taaataatta tgctctataa atcagttaaa agtttttatt tgtcttattg
    35701 aattggttat aatattttag tatagttaaa aatagtgtta ctttaaatat ttaaagattt
    35761 taaatagaag agaggtgcca tattggatta cttagagtat tttgaaaaag ctgtaattta
    35821 tattgaaaat aatttacaag aaaatataac tgtaaatgat gtggcaaaag agtctggata
    35881 ttcttattat catttaacac gcttatttaa gtctatgttt ggtgagagtg taggaagcta
    35941 cataaaaaaa agacgattag taaatagcac aaaagaactt ttatacagtg ataaaaaagt
    36001 gatagatata gctattggta gcgggtttga atcttcagaa gcatttagta gagcttttaa
    36061 atctatatac aaagttagcc ctatagagta tagaaaaaat agaattgatg tatttgttgg
    36121 taacaagaag aaattagaat tagactttat gagacatctt atcggaaata taacgattaa
    36181 accagttata aaagaaatag atgaaataaa gataataggt ataaaagata aagtgatact
    36241 agaaaataat tcattacctg aactatggga taagtttaga aaggtacacc aaattgttcc
    36301 aaatacttta ccatcagaga gagtttttgg aatctgtgaa gcaacatctg aaatacacct
    36361 aattagtgaa agtatggagt ttaatgaaat aataggggtt gaagttagta gttatgaatc
    36421 gattccaaat tcatttgttt caaaaactat tacagaagga aagtatgctg tatttactca
    36481 tactggtagc ttagagaatt tagataaaac ctatgaatat atatggggta cttggttttt
    36541 aaactcaaaa gaagagtttg atacaagaga tgattttgaa gtttatgatg aacgattttt
    36601 aggtcctaat aatataaatt cacaaattga tatttatata cctataaaat aatctataag
    36661 aaactaaaaa ataatttgat taaaaaatag atgcttgaaa tagcatctat ttttttattt
    36721 ttatcaagtt taaataattt taacaataaa agaaatagaa agaagttctt aaatttctaa
    36781 taagaattta gaatatagtc gaaataaaaa ttatatattt ctgaaaattc taattagtac
    36841 aattttttcc gttgtaaaat tgaaaaaaga tgtatttatt tatcaaaaat attatattaa
    36901 aatgatatta ataaaaaaaa catttataat aatagcgtat agtaaagaaa aaagaattaa
    36961 tttgtaaaat ttatggtgag tagtaaaatt aaaaatttaa aaacgttata gggaggttat
    37021 aattttatgg aaaacgttat tacaggtcta agtaaaggtg cagagtggtt tataggtttg
    37081 tttcagaaag ggggagaaca atttataggt cttgtttcag ggactcttcc aacactaatt
    37141 gtattaatgg tagctattaa ctctttgatt aagataattg gtgaagaaag agtcaataat
    37201 tgggcttcta agctaggaaa aaattctttt acaagatata ttttacttcc gctagtatca
    37261 gtatttttct tgggaaatcc tatgtgttat acatttggta gatttttaaa agaagaacac
    37321 aaagcaggtt tttatgatgc agcagtttca tttgtacatc caattacagg gctttttcca
    37381 catgcaaatg caggagaact ttttgtatgg ttaggaatat cagcgggttt gactacttta
    37441 ggtaaagaaa ctactacact ggcattatgg tattttatag ttggtctaat tgtaattttt
    37501 ataagaggta tagtaacaga aaaaatgtat gcttttttaa caagaaaaaa taaagtagca
    37561 gataaggctt aggaggtaga taaaatggat gaaaataaaa tattaaaaat agaaaaagga
    37621 acttcaggtt ggggaggtcc tttatatata aaaaaagaag gcaatagaaa taagatatta
    37681 tcaatgactg ctggtggtat ccatgaagta acattaaaaa taaaagaact actgggttgt
    37741 gaagttgtag atggatttaa aactggagtt tctgatgaag aagtggcagt ggttattatt
    37801 gattgtggtg gaactgctag atgtggagtt tatccaaaga aaaaaatacc gacaatcaat
    37861 gtaaatccag ttggaaaaac aggtccttta gcaaaattta taacagagga gtattatgta
    37921 tcagatgtta gtcctaactg tataagtgtt gtaaatggag aagatatgca aaaaaaatct
    37981 caagaaaata aaagtgaaaa taagagttct attagaaagc ctgataatta tgatgaggtt
    38041 aagtcaaaag cacaaggaga atatgctaaa aagaatataa tactttctat tggtcaagga
    38101 gcaggacaag tagtatcaaa attttatgat gcaggtcgag atactattca aatggtaatg
    38161 aataatgtaa taccatttat ggcatttgta agcatgctta tgggaattat tttagcatct
    38221 ggattgggag attggattgc aaaagtcata tctccacttg caggtaatat cggtggatta
    38281 ttgattatat caataatttg tacacttcca ttcttatcac caatattagg gcctggagca
    38341 gttatagcac aagtggtagg aactcttgta ggtacacaga ttggtcttgg agcaatacca
    38401 gcatatttag cgcttccagc tttatttgca ataaatggtc aagctggatg tgattttgtc
    38461 ccagtaggat tgagtttagg agaagcagaa ccagaaactg tagagtatgg tgttccagcc
    38521 ctattttact ctcgtttaat aactggacca atatctgtta ttattgcata tggagttgca
    38581 gtatttgcat tgagataata tttaatgtca atataaggct tggaattaat tactaaaatt
    38641 gtaaatataa atttaagcta gtcaagattt tttgttaatg aaagactagc ttttatacta
    38701 ttaattagct gtaattatcg agttagatta atactaaatt ttcctttatt aaagtgaaat
    38761 tttatgtatt aatttggttt caagtttgta ctagaatgaa atcaaggagg agatacaaaa
    38821 tgagtgtaac tgagaaaatt ccttctattg accacaagat atttaagata ttgattatgt
    38881 gcagtaaaaa agagtttgtg agtattaatt caatagcaaa tgaattgaat gtcacaacta
    38941 gaagtgtaag gacgtatata aaacaattga ataaagattt aggtaatgat atagcaataa
    39001 ttaaatatat aaagggtcaa ggctataaac tggaaataaa agatgagcag atactcaata
    39061 agattattga tataaataga aaaaatgtat tttcattaaa ttcaaaagaa gatagagtag
    39121 agtttatact aaattacttg acagaactag atggatttat tacacttgat agtttagctg
    39181 atgagatgtg tgtaggaaga actactttag taaatgattt tcagtatgta gaaaaagttt
    39241 tggcttcata taatttaaac ttaattaaaa aacagaatac aggtatgaaa ttaaatggaa
    39301 atgaacttga tattagactg tttatattaa atcagttgta taaaaattca aggaaggatt
    39361 tcaataactc aaagtatttt aaaggaatca aaaaagaaga aatcataaat ttagaagaaa
    39421 aactattgac tttatttaaa aaaaataact tttatgttac agatgaaatg ttaagagagg
    39481 ttattaacta tatagtagtt ttagtctata gagtaaaaga aacgaagaaa gtaaaggact
    39541 atgatgtaaa gtttgattta ctcaaatcgt atgatgagta ctttattgct agggaaataa
    39601 aacatataat atcagaaatg tttgaatgta ttttaaatga tgaagaaatt atttatttaa
    39661 ccataccact agtgagtgga aatgcaccag catcagaatg tgctttaaat agtagtagaa
    39721 ttaataaaaa tattgatgag ttgatggaaa gtatatttaa tcaaatatat gttgatatgg
    39781 gtatttttat aaatgaggat gaattaagag taggattggg atatcatctt agttttactt
    39841 tgaacaggct attatttaat ataaagttaa aaaatgtttt gctagaagag attaagcaaa
    39901 actatatact tccatttaaa ttagcacaaa tagcaggtaa agtaatagaa aaaaaatata
    39961 atttagaagt ttcagaagat gagattgggt atatagcaat tcatttcagt ggatatttag
    40021 aaagaaatag tagtagattc tattcaataa agaaaatagc aattatatgt agtacaggtc
    40081 taggaacagc aaaactatta aaaataaggg tagaaaaact gataggaaat aatcctaaaa
    40141 ttgacactct ttcaagcttt aatttgagaa atattaattt agatgaatac gatatagttt
    40201 ttacaacgat tgatttagac acatctaata taaatacaat agtattaaaa ataaatacaa
    40261 tatttgatga aaataaacta agagaacaat taaaaacagt actatgccta agagaaggaa
    40321 atattgatac tactaactct acaaatttac taataaataa tttgttagat gaagataaat
    40381 ttatgatatt aaatgaaaag actatacttg attcattaga aaaaatgatg gataatctta
    40441 tggatttggg atgtattgat gataagttta gaaaaaatat attcaataga gaagaaaaat
    40501 ctcctacagt atttgataaa ggtttattat ttccacattc tgtgaatgaa aaatcagata
    40561 aatttttaat ggcagttgga attttagaag agccaataaa atatgcaaat agaaacataa
    40621 aaataattct tatgacaatg tttccatgtg aaaataagat ggattcagac ttattggtaa
    40681 aaatttatga agaaatttta aagatagggc aagatgtaaa attgacaaat aaaataagta
    40741 agtgtagaag ttttttagaa tttaaaaagg tattacttaa aaaccttatg taactactgt
    40801 acagaatcta tagttattgt acagattaaa agaataatgt gatttttgga aggtgtataa
    40861 atatgaaagt actagtgatt ttatttgctg cttatatttt gaataccata cttataatga
    40921 agcagaataa gttgtatttt aaaactttaa atagagctaa aaaaaagggt gatatagtgt
    40981 ccacaggcaa aaagaaaagc tatttttcta aaggaagtat agccattata tcttcagatt
    41041 caaatggatt tataaagttt ggagagattt taaaaggtcg tacagtaatg gctaagttta
    41101 aagagattga agacatagaa ggattagata tatatagtgc agagcaaaaa tttaaaaatg
    41161 aagaaagtat tgtacaagct ataaaattta taaaagaaaa aataaatgtt tcttttaatt
    41221 aactcaatat aaatataaat tatcaggagg aatgtcatga aatatgaagt taagataagt
    41281 ggaataggaa gtttagttaa agagttaatg gatgaaagta attgtcttat tatatatgat
    41341 gaaaccatta atgatgacga cttaaaggat atatctgtaa ttcattcgat atcgacatta
    41401 agaagtgatg ttgaaatagg ggatacactt actataggaa atagagatta ttgtatagtt
    41461 tcagttggtg atattgctca aaagactctt agagaaattg ggcattgtac aatcaaattt
    41521 gatggaaaaa atgaagtaaa tttaccagga gaaattcatg tggaaatagg aaatccagat
    41581 ataactattg gagatttaat aactatttct taatttgaaa ataaaattat tataacagga
    41641 ggaattggta tgttagattt aaacagaaaa cttgcaaaat tggaagagga aggtaaaaag
    41701 ataagagtag ctcttatagg ggctgggcat atgggaaatg gaatggtaag ccaaatggca
    41761 aatatgaaag gtattgaagc ttctattgtt gtagatataa atttggagtt ggctcataaa
    41821 gcttttactg atgcaggaat agatgaagaa attatagaca atgtaacaaa tgaaaatgat
    41881 gcagaaataa aattacaaga aggaaaagta ttgacatgta atgattttct tgtagcttgt
    41941 aaaactaagt caatagatgt agttatagat gcaactgggg gtatcgcaat tggtgcagag
    42001 atagcattaa actctatttt aaataaaaaa catatagtta tgttaaatgc tgaaactgat
    42061 tgtgttgttg gaccaatact taaaaaattg gcagatgatg ctggagtaat atttacaggt
    42121 tctgcaggag atgagccagg tgcagtaatg gagttatttg attttgcaga tgcaatggga
    42181 tttgaagtta gagtgatagg taaaggtaaa aataataaat tagatttaga ttgtaaccct
    42241 gatacagtta gagaagaagc agaaataaaa ggagcttcac ctcatatgat agcttctttt
    42301 aaagaaggaa ctaaaacaat ggtagaaatg gctttaatgt gtaatgcaac tggatttgta
    42361 cctgatgtaa gaggcggcca tggcattgaa gctactgtta atgaagtacc taagaaatac
    42421 gcattaaaaa gcgaaggtgg agtattagat aactatggtg tagttgattt tgtaaatggt
    42481 atagcacctg gagttttcgt agtagtagcc cataaattaa aagcagttaa tgatgaatta
    42541 aaatatttaa gtatgggtga tggacctaat tacatattat atagaccata tcatttatgt
    42601 agcttagaga ctcctttatc agcagctatg gcagttttag aacataaagc tacaatagtt
    42661 cctaaagctg gattagttgc agaagttatg actatagcta agaaagattt gaaaaaaggt
    42721 gagtatatgg atggctatgg agcatataca tgctatggca ctatagaaaa atacgatgta
    42781 gcaaaggcta tgaaagctgt acctattggt ttgataagta aaaagactaa agttgtcaaa
    42841 gatattaaaa aaggtgaagt tataacttac gatatggtag agatagaaaa agatactact
    42901 ctatatcatt taagacaatt acaagaaaaa atatttgggt aagatttgat agtaatataa
    42961 atggatatta acaaaaaggg ttgatgatta tttttaaatc aaccctttta aatttttgta
    43021 tattcttgtt aggatacttt ctttagaatt atattatagg ttatttgtag ttataaaaat
    43081 tatttttaat tagttctgca attttatatt catattaaaa taatattatt aaaaatttta
    43141 atataaaatg aattaaaatg accaattatg tattataata ttaactgtaa tactaatata
    43201 gttctattaa ttaaagaaat atcccccatt aatatataat aaatatcttt aagcgagaga
    43261 acttagtaaa aaatatatcg tattcaataa aaaatctgtt tacagaatgg tatatatagt
    43321 tatataatat actatataag atgcgaaaag ataaaaagta tagcacaatg aaagaaggtg
    43381 attattattc agcttaatga aagacaacta aaaattatag atatagttaa ggaaaatgaa
    43441 ccaataacga gtgaaagtat tgcctccagc ttgaatgtga ctcgtgctac acttagatct
    43501 gatttagcta tattaacaat gacaggaata ttagacgcaa gaccaaaagt tggatatttt
    43561 tattcaggag ttagtgaaat aaatttaatt ggtaagagtg taaaagaaaa aacagtagaa
    43621 gatataatga gcatgcctgt actagctaag aaagacgaaa gtatatatga tgtaatagtc
    43681 actatgtttt tatctgatgt agggagtata gttattatag atgagaatga agagttatgt
    43741 ggagttgttt ccagaaaaga cttgttaaaa gcaactatag gaggttctga tataaataag
    43801 atgccaatag gtatgataat gacaagaact ccaaatgtag taactttaac taaaggagca
    43861 agtgttttat tagcttcaag aaaaataata gaacatgaag tagattcgat tccaattgtt
    43921 gaatataagg gagaagacaa aaatcatatg agagttgttg ggagaatatc aaaaaccaac
    43981 ataactaagc tattcttaga aatagttgac aattaaggag gtatgctggt gaaaaaccta
    44041 attatatatg cagtgtcaga ttcagtaggg gagactgcac aacaagttgc aaaagcatgt
    44101 atgtctcagt tttatgtgaa tgaaacttat gaaataaaaa gatttccata catgattaat
    44161 aaaggagtac tacttgaaac tttggagaat gcaaaagctg aaaacgcttt gattgtatat
    44221 acattagttg atgaagaatt atgcagtatt gttgaaagat attgcgaaag agaaggactt
    44281 agttgcatag acttaatgac agatatacta agagaaataa gcaagagaac aggaagaaaa
    44341 cctaaaagag aagctggtat aattagaaaa ttagacgaat cttattttaa gagagttgaa
    44401 gccatagagt ttgcagtaaa atatgatgat ggaaaagacc ctagaggtat tcttcaagca
    44461 gacataattt tagttggaat ctcaagaacg tctaaaactc ctttgagtat gtatttagct
    44521 aataaaaata taaaagtagc taatgttcca ttagttccag aaataccaat tcctaaagaa
    44581 gtgtttgaga tagatactaa gaagataata ggtcttacta attcacctga aaagctgaat
    44641 gagataagaa ctcaaagatt aaaagcctta ggtttatcta gtaaagcaaa ctatgctaat
    44701 ttagaaagaa tacttcagga attagattat tcagaagaaa taatgaaaag aattggatgc
    44761 ccagtaataa atgtttcaaa taaggcaata gaagaaacag caggtataat tttagacata
    44821 atgaaagaaa atggtctaaa gatatacaaa gaaatagaaa tttaataatg tctattaata
    44881 aaaatgatat aaattaaaat aaaaagatta atgggggatt taacaatgga aactaagtat
    44941 gtttatagtt ttggtgaagg aagcaaagac atgaaatctt tactaggagg taaaggtgcc
    45001 aatttagcag aaatgactaa gataggctta cctgtccctc ctggattcac tataacaaca
    45061 gaagcatgta atgattatta tgttaacaat gaaagtataa gaaaagagat aataaaagaa
    45121 atagaggagc atttagctac tttagaaaaa gatttaaata aaacattagg atgcaataag
    45181 aatccattat tagtatcagt tcgttctggt gcagtattct ctatgccagg aatgatggat
    45241 acaattctta acctaggtct taatgacaac agtgttgttg gtctagctga ggctacacaa
    45301 aatgaaagat ttgcatatga tagttacaga agattcatac aaatgttttc tgatgttgct
    45361 atggaagttc caaaatataa atttgaaaat gtattagaca gagtaaaaga agcaaaagga
    45421 tatacagttg atacagagct tacaactgat gatttaaaag aaatagtaaa agaatttaaa
    45481 gcaatttaca aaaaagaaat aaaaaatgat ttcccacaag acccaaaaga gcaattaatg
    45541 cttgcaatag aggctgtatt tagatcatgg aataatcctc gtgcaatcgt ataccgtaaa
    45601 ttgaatgata tagctcataa cttaggaaca gcagtaaaca ttcaatcaat ggtatttgga
    45661 aatatgggag aaactagtgg tacaggtgtt gcatttacta gaaatccagc tacaggagaa
    45721 aataaattat ttggtgaatt tttaatgaat gcacaaggtg aagacgttgt tgcaggtata
    45781 agaactcctc aaaatatatc aactcttgca gacgtaatgc cagctgtatt tgatgaattt
    45841 gtaaaaatta ctcatatact tgaaggtcac tataaagata tgcaagatat agagtttact
    45901 atagaaaatg aaagattata tatattacaa actagaaatg gtaagagaac agcagcagca
    45961 gcaataaatg tagcagttga tttagttgaa gctggaataa ttgatgaaaa agaagctata
    46021 atgagaattg aacctaatca attagaccaa ttattacatc caaaatttga agataatgcg
    46081 ttaaaagaag ctaaggttat agcaaaaggt ttaccagcat caccaggtgc agcaagtggt
    46141 aaagtttatt tcaatgctga tgatgtagta aaagcaaatg aaaaaggaga aaaagtagtt
    46201 ttagttagac tagagacatc tccagaagat atagaaggaa tggttaaagc agaaggaatc
    46261 cttacagcta gaggtggaat gacatcacat gcagcagttg ttgctagagg tatgggtaaa
    46321 tgttgtgttg ctggatgtgg agaaataaaa gttgatgagt ttaataaaga aataagagca
    46381 cttgatgatg tagttataaa agaaggagaa tatatttcta tagatggttc tacaggaaat
    46441 gtgtacctag gagatgttaa gaaaacagaa gtatctttaa ctggaaactt tgaaaaatta
    46501 atgaactggg tggataaaca caagtgtatg atggttagaa ctaatgcaga taatcctaga
    46561 gatgctcgtg cagctataga atttggtgct gaaggtatag gtttatgtag aactgagcat
    46621 atgttctttg atgaagctag attgccagca gttagagaaa tgatattatc taacacagta
    46681 gaacaaagag aaaaagctct tgaaaaaata ttaccaatgc aaagagagga ttttgtagag
    46741 ttatttaaag ttatggatgg aaagccagtt aatataagat tattagaccc accactacat
    46801 gagttcttac ctcatgatga tgagactata gaagaacttt caaaatctat gggaataaaa
    46861 gtaagtgata ttaagaagag aatagttgat ttagatgagt ttaacccaat gcttggacac
    46921 agaggttgta gacttgcaat aacttatcct gaaatatgtg taatgcaatc taaagctata
    46981 atacaaggtg ctatagaagc aatcaaagca ggggttaaag tttctccaga gataatggtt
    47041 ccattagttg gagaagtaaa tgagttaaaa ataataagaa aaatgatagt agaaactgtt
    47101 gatgctataa taaaagaaga aggcgtagaa gttccttata ctgtaggtac tatgatagaa
    47161 atacctagag cttgtttaac tgctgatgag atagcacaag aagcagactt cttcagtttt
    47221 ggtactaatg acttaactca aatggcattt ggatattcaa gagatgatgc tggtaaattc
    47281 ttaggtcaat atgtagatga agagatatta gaaaaagacc cattccaagt actagaccaa
    47341 aatggtgttg gtaagttagt taagatggga gctaaattag gtagagaagt taaaccagaa
    47401 ttaaaattag gaatatgtgg agagcatgga ggagaaccat cttcagtaga gttctgttat
    47461 agtgtaggac ttaactatgt atcttgttca ccatttagag tgccaattgc tagacttgca
    47521 gcggctcaag catctataaa aaatcctaga taataataga atgtaaaata tgtaactaaa
    47581 taaaaataat atcccctatt gttcaatgta tcaaatctag tatttgtaag ttgaatatag
    47641 gggattttta ttataaaaat ttaacttata tggttgtaag tgaaacgtat ttaattatat
    47701 tgcatatatt attgtagaga gaaattttaa ctattaatta aattgagttt actggagggg
    47761 ataaagttga caaagtgtcc taaatgtgga aaggaagtat catcacgtcc aggtagtata
    47821 tgcccacgat gtggatttaa ggtagcagat gagagtctaa aaataagatg tccagaacct
    47881 agttgtaggg ctttggtatc taggaaactg gattattgtc ctaaatgtgg atgcaaactt
    47941 agaggatata atatttctga atttgttaac atggtgagat gtaaaataga tgagacattt
    48001 gataaaagat aattaaattt aaagttaaac ttttgagggt gtctcaaaat gaacttttta
    48061 gtttatgaga cactcttttt tgtatgaaat caaatatatt tttatcattt aaatagttaa
    48121 aaagaggctt atctccattt tgagatagcc tctataaaag tatacagttt tataagtaaa
    48181 tatatttttg ctaatctatt tctattattt gccttatgaa tcttccatag ctaaagtcat
    48241 aagtgtgcat agctattttt taatcaaatg tatgttaaag taatctttct atttaccaga
    48301 attactttcc agtattaagt atccaaggtt tctctatacc attttttagt gagttataat
    48361 tcacaataag tgtgtcatag ttatttttag catcttcatt gcttacaact tgttttttat
    48421 aatcagttag agtaattaat cctaaatcat atcttaattt agtatttttt aattgttttt
    48481 ctgatagttc aagttgtttt ttagtaatct gaattgtatc ttctaaagca agtaattgag
    48541 cataactgtc aattaatcct tttttaagag cttttttatt gtcttctaac gttactagac
    48601 cattttcagt attgtatttt tgttgaagat aattcgcata agtagtaaga ccagcagcat
    48661 aagtctgtct ttcaagattg tattcttcaa cttctttttt atatctatca taatcttttc
    48721 catacttatc cataagaacc ttgtattcat catcagttag attaccttgt tctggttttg
    48781 taggagcaac tggtcttgta agattaggtc tatctggtaa atctgctttg ttctcatctt
    48841 tatcattaaa actttcttct gtacgttcta aaatcaattg gtcgtatttt aaatattcat
    48901 taaccttgcc ttccatatat gaatcaactg agccacctac tctgaaagat tcaaatctat
    48961 attctttatc taattgataa ttatttaaat ctatattagt caataattta aaatagtctt
    49021 gattattttt tagttcttga agcttagctt tttgagtatt ttgtagtgtt tgaagttcaa
    49081 gttcagctga ctcagtttct aaagatgtta ctagccctaa atcttttttt aatttcatat
    49141 ctttaatttc tttagttttt atctcaagat tattttttat cttatctaac tcattttctc
    49201 ttgaaactaa atcgttaaat ttattagtaa tatcattggc tatttggtcc tccataaaac
    49261 ctttagcctg ttcactttga tttttaagaa gtccaatttg gtcatatgga aatgagttgt
    49321 cacttccagt aaaactatca aattcatctt gcacttctaa cttttctttt aacattttta
    49381 tttcttctga tttcattttt aatttttcac tatttttaag tgcagcacta acagcttctc
    49441 tagcagttaa aatttccttt tcagatgttc ttttagttga gtcactcaca atggaattat
    49501 caactttatt tatagaagaa gtactattat ctgcataaac gggtattaga ttagctgtta
    49561 ttataccaac gccacagcca attgcaacta ttttgctaac ttttttattc aatacaatca
    49621 ccctccattc tatttaaact ttagctttta attttggttt ctttattatt tttcttttta
    49681 tatcacctat tttatctgtt attttaccaa atatcttagg cattttgtct gaaaacttat
    49741 tttcccattt atcaaatatc aagtatatag taggtataag taccaaagtt ataagtgtag
    49801 atatagtcag acctcccatt ataactattg ccagtggttg cattgtttct ccaccttcac
    49861 caagagatag tgccgttggt aacataccta aaattgttgt tgctgtagtc attaggactg
    49921 gtcttaatct tgctgcacaa ccaattgaaa ccaaaccaat taaatcatta ttacgatttg
    49981 tttttctaag ttgctctatg tagtcaataa gaactatacc attgttaaca acaataccta
    50041 taagtaagat tatacctagc atacctacag tacttaaact ttgtccacta actagaagag
    50101 aaagcacaac tccaacgaat gcaaatggga cacagaacat aattataaat ggtttactaa
    50161 atgattcaaa ctgagctacc attaccatat aaactaatac tatagctatg agcattgaaa
    50221 atccaagact cgccatagat tcattcatca tctctgtttg accaccaaca ccaatagaat
    50281 aatctttagg aattaccatt ttattcgttt tttctagtgc tagttttgta gcagtaccag
    50341 catctaaatt gtcaagtgta gcagttatat ttacagagaa atttccatca gttctagata
    50401 tagactgtaa accatctgcc atttttatat ctgaaaaagc accaagagga acttcttttc
    50461 cagttgtaga gtttactttt aattgtttta tgtcttctag attatcaata ctatctgctt
    50521 taaattttaa atttacatca accttgtaat catcaatagt ggcagtagta actgaatcac
    50581 catttattgc tgtacgcagt aatcctgcta tttcagcagt gtttatacca tattcttgag
    50641 ctttgtgttt gtctattgta aattgagctt cttgtgttgt atctgtaaga gaagtctcaa
    50701 catttctaaa tccttttata gagctgaatt gtttttcagc ttcttttcct aatattttca
    50761 atacatccaa atcagggcct ttcatttcta atgtgaaatc tgctcctgaa gttccaccca
    50821 tgacactact attttgacta acatttattt tacagtctgg gacagtggca actctatcaa
    50881 ttatttcttt ttctacctca tcagtacttc tctttctatt tttttgtgat tctaattcaa
    50941 ttgctatatt tgctgtatta ttatttgaat ctgatgtcaa agttgttatt aaagttttaa
    51001 tctctggaat atctgaaact tttttttcag tcatagatac atagtaatca ttagattcta
    51061 gactaagacc ttttggaagt tctatagaaa tagccagttc acctttatct gcggcaggca
    51121 taaagtccat acctataaag ctcattccaa ataaagaagc tataaaaata agtaaacttg
    51181 atattactac taagcgttga tgtcttaagc atacacttag taatctttta tataactcac
    51241 ttactttatc aaataaagga gtatcttttt cttgcatttt ttgactattc ttgcctctac
    51301 ttaattttgc aaatacacta gggacaagag taattgcacc tatgaaagag aaggctaggg
    51361 aagctattag tgttttacct aatgcaccaa aggttatttt ggctatacca tctgtaaaca
    51421 taataggtaa aaatattgct atagtagtca atgttgatgc aagtattgca tttgttacag
    51481 ttcttgttcc ttcaatagca caatcatcaa tactcaatct accgcttttc ctatatttaa
    51541 atatattttc aattacaact gtggcattat caacaaccat accaaccgaa attaccagtg
    51601 aacttgcagt aataagattt aaactttcac cagtaaagta gagaattgct atagcaccta
    51661 ttatagaaat tggtattgca atcgcaacaa ccagtgaagc tcttacactt ttcaagaaag
    51721 caaagattac aataaatgca ataacagaac ttataagcaa gttgttcatt acactgttta
    51781 ttgattcact tatgtatgaa cttgtgtcgt tagcaatttt aattttaaat tggggatttt
    51841 ctttatttaa ttcatccaca tatttgtatg cactttgcat aacttttaca gtattggcat
    51901 cttgttgttt agaaatatct attaccaaac tgtcttttcc attgtatctg taaatactat
    51961 ctttgtctgc atatccataa tctacactac aaagattatc taatctaact gtatttccac
    52021 cttttacagg aatttgaatc tgttttatat catctagcga ttccaactta tcaattgctc
    52081 ttaaaactat tttatcttca ccttgagtaa ttgaaccata agggaatgtt ttattactag
    52141 aggatagtac accttttata gtggataaac taactccata atttgaaaga acagctgggt
    52201 cagctattac attaacctgt gctttttctc cacctttaag gtctgctgat gtaacaccat
    52261 ctatggattc tattttaggt tgaatcacat cttcagcata ttttcttaat gcttgtgaac
    52321 tattttcatt tcctccagat ataacaagtt gacctatagc ttgtgcattc atatccaact
    52381 ttaaaacagt tggtttagct gcatcatctg gaagactagt ttgtaatgca tcaactttag
    52441 acctaacatc attcattatt tcatctaaat tagttccaaa ttcaaattga gccactacca
    52501 tagaaacact ctcttgtgaa cttgatatag tagattttac atttgaaaca gctgataaac
    52561 tttcatcaac tttttgacca atttgttcat caacatcttg aggaccagca cctgtccaag
    52621 tagtcatgac cattacaacg ggtatttcta tatctggcat cagatttatt gacattttct
    52681 tatatccaat accaccaaaa actattaata ctaaaaatac cattataatt gtgagaggac
    52741 gtttaacgga agtttgtgtt aaattcatag cctatttctc cttttctaca ggaaatatct
    52801 tagttccatc agaaataaga cttatccccc caataacaac tgtatctttt tcacttacac
    52861 cacttttaat ttctattttg tcctctgtta caattccagt ttgaactaat tttttctttg
    52921 ctatattatc cttatttgct atgtatacat atttctttcc tttttcttca aatatagcct
    52981 ttttaggtac tgtaacggca ccattttctt tttcaacact taagtttact tgggcagaca
    53041 ttcctgcttt aaatttgttg tttgtgttat taacaagcac tttaatagga tataaagatg
    53101 tctgtgggtc aactactgaa ggtacataat ctatttttcc taaaattctt tggtcttcaa
    53161 ttataacttc tacatttcca tcctttttaa atttatctat atctgattct gctacattta
    53221 aattaacttc taatatgtta ggattagaaa taataaaagc tggttgtgat tgtgttatca
    53281 tttcttttgc atcaaaattt ttggctgtta ttgtaccatc tactggagct gttaatgtta
    53341 atttatctaa attactcata gctaaattat atgatacttg tgcttggtca agttgttttt
    53401 tagcaagagc tttagcttgt ggtatactct ttgaagttac tgtatcgtat gatctttgag
    53461 cagaatctag ttgttgcttt gattgttcta aagcaaattc tattttttta aattggtcta
    53521 aactaattac ttcctgctca taaagaatct tattattgtc ataattcctt tgagattcat
    53581 catattgaat ctttgcatta tcaagtgctg tttttgcttg atttacttgt gcttctaatg
    53641 aaccacccgt tgtacttgaa taacttgctt tagccaaatc tagtgctgct ttactttgtt
    53701 ttacactatt ttctagatct ggactgctta tactcaataa ttcatctccc tttcttacat
    53761 tttgcccaag ttttacataa acttcttgaa ctgtgccaac agtctgtgca gtaacagctg
    53821 tttcatcttt aacctttgta gttcctgaaa aagcatttgt attttcaatc tctcctccta
    53881 ctgctttttg aacagaaact gctattggtt tctcaggctc aggttcttct gtttttccac
    53941 atccaactaa aaacagtgaa ctacacatta agaaaactat taaatatctt ttcatatctc
    54001 ctcctattat atattgcaca ctaacttgtt gtagaattta aagtgtctca taattcaagt
    54061 ttatttttag gctaattaaa aaactttctt ttattggcaa tttataattc tctaatttga
    54121 ttataaattt ataattatta tgagtcaata actttttctt gttttaaatt tgttaatagt
    54181 ttataattta tattatataa gtaacaaatg ttttattaca tcatttattg aagggatgtt
    54241 acaaatatgg ataataacaa tatacttaaa atagcagact cttttttaga ctttttattt
    54301 gtgcttgaaa ataatatatt tagagaaagt gatttgttaa aaaaatttca aaataactct
    54361 gatataatga aagcgtattt tgaagaatgt ccaatggctc catcacatgc taaggtaatt
    54421 ctttatctca tgacatcaaa ttcatcatca atatctcaaa tagcttctaa tttaggaatt
    54481 ttaaaatcaa acatgacacc gattattgac agattagtgg aacatggttt agtaaataaa
    54541 tttcctgacc caaaagatag aaggattctt agggttgaat taacggataa agcatttgaa
    54601 ttatttggcg cagtaaaagc tattctcaaa gaatctctag taaaaaaatt atctaatctt
    54661 tctgaggagg aactaacttt attagatgaa catacattaa agttatcaga aatagtaaag
    54721 aaattaggtt agcatgtggt tgtaaaagtc attattaaaa aacttaaaac ttcataag
//
LOCUS       DAFNNB010000006        53221 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0006, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000006 DAFNNB010000000
VERSION     DAFNNB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 53221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53221
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0006"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(101..727)
                     /locus_tag="L3M60_000324"
     CDS             complement(101..727)
                     /locus_tag="L3M60_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBN6225031.1"
                     /translation="MKYEWRKRDKELYLPKKNPAIIEVPEQKFIMLKGNGDPNSKEFT
                     EAIGVLYSLAYAIKTMPKKGIVPEGYFDYTVFPLEGIWDKGEKSKCLDVLIKEDLIYT
                     LMIHQPDFVTQDLLDKATDIVKKKKPHNLLEKVRFGSINEGLCVQMLHIGSYDNEPES
                     FDIMNEFCKKNKLIRKSYIHHEIYISDARKVSPESLKTVLRFEVEREV"
     gene            complement(804..1775)
                     /gene="nrdF"
                     /locus_tag="L3M60_000325"
     CDS             complement(804..1775)
                     /gene="nrdF"
                     /locus_tag="L3M60_000325"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBN6225032.1"
                     /translation="MTFNLHKIHNAVNWNREEDGFTQAFWEQNVKQFWLPEEISVSKD
                     IKIWNELSNKEKELYKKVLGGLTLLDTKQGNNGIPSMMSLTENLQRKAVLSFMGTMEE
                     IHAKSYSSIFMTLLSNSEIDELFEWIETEPTLQRKADLVLAQYENTTNQEGLYLSMVT
                     SVFLESFLFYSGFFYPLYLSGQGKMVASGEIISLILRDESLHGKYIGLLAQEIYDSFD
                     KMDKEILEEKMYSILYRLMENEIEYTNIIYRESGLEKEVVNFLKYNANRALENLGFEK
                     LYTVDAINPIVLNGLSTETKTHDFFSTKGNGYQKGVYEELEDEDFII"
     gene            complement(1788..3887)
                     /gene="nrdE"
                     /locus_tag="L3M60_000326"
     CDS             complement(1788..3887)
                     /gene="nrdE"
                     /locus_tag="L3M60_000326"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBN6225033.1"
                     /translation="MNWIELNNQVIIKNEQGKYQLEKDREALSSYIEEFIQPKLRKFN
                     NLEERLKYLIDEGYYSKEVINQYSMDFIKNIYEIIDKQKFEFQSYMSANKFYQNYALK
                     SNDGKKILETYNDKVLIVALTLGNGNKDLVLNLADKIVKQEFQPATPTFLNAGRKRAG
                     EMVSCFLLSVEDSTEGISYAISSSNHLSKIGGGVALNLSKLRASGESIKDIEGAAGGV
                     VGVAKMLEQSFSYFNQMGARQGSGAVYLTVFHPDFELLMDTKKINADEKIRLATLSLG
                     AIIPDKFMELAEKNEVAYAFYPHTVYKKYGVSLDEIEMNKWYDKLVNDNDIRKKEINP
                     RQMLTKIAQMQQESGYPYVVYIDTANREHTLKDIGIIKMSNLCCEIFQYQTHSEIEGY
                     GGKNEWGQDISCNLGSLNIANVMDNKTIESTVETAIRALSFVADSTDIKPVPTVSNSN
                     SKSHSIGLGAMNLHGYLVRENILYTSDDAIDFSNVFFAMVRYYSIKASMKIAIEKNQT
                     FEGFDKSEYAKGRNSKVLSKYYEQSYLPKSEKVKALFEGIYIPTKEDWTKLLDEVKEK
                     GIYNAYLMAIAPTQSISYVQNSTSSIMPITEPVEVRTYGDSTTIYPMPFLTNDNILYY
                     QSAYRMDMRKVIDLVATVQNHVDQGISTTLFVTDEKTTRDIARHYIYAYKKGLKSLYY
                     TRTKMTRDTHECLVCSV"
     gene            4365..5090
                     /locus_tag="L3M60_000327"
     CDS             4365..5090
                     /locus_tag="L3M60_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBN6225034.1"
                     /translation="MKTISKQELEKTLEKHYLWLKNNNTGEKADLKELRLENMDLRGY
                     CLSNIDFSWSDLINLNLEKADLENSIFNNSYLSDCSLKNSILKNADLSGANFRFCDLS
                     NSDIRGANLENSNLEYATLNGVISDKSTRFFKLYCPETGAFIGYKKCFNFRMVQLLIP
                     SDAKRVSATSNACRCDKAKVLSITSIDGKESFKSARAYADENFIYRVGETIVAKDFNE
                     NRWKESTTGIHFFLTREEAIGYL"
     gene            complement(5300..6496)
                     /locus_tag="L3M60_000328"
     CDS             complement(5300..6496)
                     /locus_tag="L3M60_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225035.1"
                     /translation="MLKTNNLSVGYNNKVVVSDINIEVKSGEILCLLGSNGAGKTTLL
                     RSLSKLISPIKGEIYLNGVNINHISRKALSKKMALVLTNRLLADLMTVQDIVNIGRYP
                     YTGFFGNLSKKDLIMVDEALESVDALHLKKRYFDELSDGEKQKVLVARALVQEPEIII
                     LDEPTTHLDIKHRLELINILKNLSKEKSISVILSLHEIDIALKSCDKVALIKNNKVIA
                     YGQPEDVVDENIINSLYELDDKNFNSLLGSVEISNKSKNEVFIIGGGGKATPIYRAFT
                     KKGIGLYSGIIHENDIDYEIGRTMGIKMFTENPFESISDESFDLAIRNLNASKIIIDT
                     GFSVGETNKRNIDIVKEALKLDKKVYSFRNRDESKKYYDSLDNKIEHVDKVSQIVDSI
                     NMNNLL"
     gene            complement(6499..7524)
                     /locus_tag="L3M60_000329"
     CDS             complement(6499..7524)
                     /locus_tag="L3M60_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBN6225036.1"
                     /translation="MDFINKMRIRNTTIFIVLIISIILTFLVCVAFGSVKIEFKEVID
                     VVLRGMDNTEHYKIIYNIRIPRVLATMVGGSCLAVSGILLQIFFKNPIVEPYILGVSS
                     GSTLSVALMILGGYTLGFESVNSMSIFVAAFIGALIVMSIVILFANKVRNITTLLIIG
                     IMTGYVCSGITNVLQSFANSDKLKEFTMWSMGSFSGFTWEKVEILITVGVLGLLMTVF
                     IIKPLNAFLLGEEYAKSIGINIKFFRVLIVFISSILAAIVSAFAGPIGFIGLAVPQVS
                     KLIFKTSDNRILIPVVIFLGSIVTALCDLISRVVLSPVELPISTITSLLGAPIVVMLL
                     MKRKDRA"
     gene            complement(7596..8726)
                     /locus_tag="L3M60_000330"
     CDS             complement(7596..8726)
                     /locus_tag="L3M60_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6225037.1"
                     /translation="MKLKSNVLKQFFLIILAIVMVGGLIGCSKNDKENKQVSNGKKLE
                     LKYASGFSVEDLEDGIKKVTDGDNRELILIPKKIKIPEKYKNANIVRTPVDNVLIAST
                     TQACSLRTIDELDSIKAVTTEEQYWTIKEIKDLMKDGKIHYVGSNLAPDYEKIVDLNP
                     DLTIVSSGLSEADTKFIDKLKELGLNYVVDNEYKEQNPFGRMEWTSLIAAFYDKEDIA
                     TSELNKTVQKIEEVSKKIKNKAKPKVVWASVYEGSAYIAPKDSYVDNMIKMAGGINAC
                     ASIDSNEGRVSIEQLHEVAKDADIFVYSSSSDYAPNLDSIKSSAPVLADLDVVKNGNL
                     WVFAPDYYQSVDKTDELIVDLVEMFHPGTTGEKIKHYINYDK"
     regulatory      complement(8956..9137)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(9190..10083)
                     /locus_tag="L3M60_000331"
     CDS             complement(9190..10083)
                     /locus_tag="L3M60_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HBN6225038.1"
                     /translation="MKAEFLTPVVTIFDEEGNLDREGNKKVYDHLIDGGVDGLVIMGS
                     TGEFFNMSMDMQKELIDLVTSHVDKRVKVFIGTSRMCINESIELSNYAHNSGADGVMI
                     ISPYYFSLDDDSIELFYSEIAKNTEAEIYLYNYPDRTGYDLSPELTLRLARKHKNIVG
                     YKDTVTLMGHTRDLINTMKAEFPEFKVYSGYDENFIHNLMSGGAGCIGGLSNLIPEVC
                     SKWAEAYNNRDMDKVIEIQKYINKAMDFYLITNPFIPAMKKAMNIRGLQISDYMTAPF
                     VKPNEKQVCEIKKLMNELNIC"
     gene            complement(10163..11065)
                     /locus_tag="L3M60_000332"
     CDS             complement(10163..11065)
                     /locus_tag="L3M60_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBN6225039.1"
                     /translation="MKFVSFTEGENECKRTGVFSKDELFIIDVNSLNLSRNFKDLNEL
                     IQNISSEDIIKLKTIINESTFYNYDVFKRERCTIHVPIEKPIHDILCVGVNYKDHLEE
                     TQTHFDNSFEDPQKTVYFTKRVIKAIGPEDEIDGHFEINNQLDYEVELGVVIGKTGVN
                     IRKEEVEDYIFGYTVINDISARKLQKEHVQWFRGKGLDTFTSLGPCILHKSSVPFPIK
                     LKVCSRVNGEERQSSNTRLFISDVSDIVSELSKGMTLESGDIIATGTPSGVGMGFSPP
                     RYMSSGDVVECEIESIGILKNYIK"
     gene            complement(11115..12275)
                     /locus_tag="L3M60_000333"
     CDS             complement(11115..12275)
                     /locus_tag="L3M60_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBN6225040.1"
                     /translation="MKFYGYKRPDGRVGIRNHILILPTSVCASDTTRIIASQVQGAVT
                     FNNQNGCSQVHSDQQLTMDVMAGYAANPNVYGVIVVSLGCENCQNDLVVDAIKERTNK
                     PIKTLVIQEEHGTLKTIEKAVRYAREMAQEASLLRKEEFPISELILGTECGGSDPTSG
                     LAANPLIGELSDKLVDLGATSILSETTEFIGAEHILARRAVNEEVKEKILHIVHRYEN
                     SLKLVGEEVREGNPSPGNIAGGLTSLEEKSLGCIHKGGHRQISEVYDYAKQINKKGLV
                     IMDTPGNDASSVAGMVAGGAQIVVFSTGRGTPSGNPIAPVIKITGNKITFANMEDNMD
                     FDASPVIYGPQTMEELTDDLLNMVVDVANGKQSKAESLGYTEMAIARVCNYV"
     gene            complement(12430..12735)
                     /locus_tag="L3M60_000334"
     CDS             complement(12430..12735)
                     /locus_tag="L3M60_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBN6225041.1"
                     /translation="MINAVIIDEKDNVAVAIEAIAKGSEISFKLKDKTIKTITALDDI
                     TIYHKLAIKDIAKGDKVTKYGEHIGEANQEIKSGQHVHIHNVRSVREDLDKQVMTNI"
     gene            complement(12833..13807)
                     /locus_tag="L3M60_000335"
     CDS             complement(12833..13807)
                     /locus_tag="L3M60_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBN6225042.1"
                     /translation="MIMDFIKKIPAGMMIVPMFIAAFINTFCPDIVQIGSFTTAVFSS
                     VGSATIIGVQLVCLGTQLQFREMPKVLKRGGILLGSKFAIGALIGILIGKLFGMDGVL
                     GLTTLAVISAVTNSNGSIYLSLMTSYGDETDCATMSLLTLNDGPFFTLIALGTSGLAN
                     VPLLSLLAAIVPILVGMIIGNFDKKMKEFLEPGCNLLVPFVGFALGSSINLASILKGG
                     LSGVLLGLISVFVGGIFIVFCDKFIGKRPGYAGWAVATTAGNAIAVPAAVALVDPSWQ
                     PYVATATTQVAAAVVVTAILVPLITSWWAKKYGCPKFPKQNEDGTITA"
     gene            14397..15173
                     /locus_tag="L3M60_000336"
     CDS             14397..15173
                     /locus_tag="L3M60_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBN6225043.1"
                     /translation="MTSDNHRPTARILDILEALAESENGYTLTEIADAINAPKSSIFP
                     IVHTLNARKYITIDKNTSKYSIGIRSYTLGSSFFEKRTIFNYIIDEMTNIVNSCSETC
                     QLGILDNNEVLYIGKVDSPEPIRLISYVGKKIPANCTGLGKALLCDFDKEKLISLYPE
                     GLKGFTEKSITDFDVLYDQLLEIQRTQIASECEESMEHLKCLAVPIRKNGCIRAAVSV
                     TLPLFRANDEKIELIKKLLLNAQKNIQSMMEERNEDIVIN"
     gene            15505..16650
                     /locus_tag="L3M60_000337"
     CDS             15505..16650
                     /locus_tag="L3M60_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBN6225044.1"
                     /translation="MSIYYVPPINLLGKGCLNEAKNSIKSLGTKKAFVVSDKFLVSNG
                     TVKKVTDILSQIDVDFVVYNEVKQNPTVTNVNKGLEILKSENCDFVITIGGGSPQDCG
                     KAISILATNGGDIRDYEGINKTSKKCLPIVAITTTAGTSAEVTINYVITDEDRHVKMI
                     MVDNNSLSTITVNDPELMISKPSNLTAATGMDALTHAIEAVVANGAYDVTDSTALYAI
                     KQIFKYLPRAVKNGNDIEAREQMCYACFLNGIAFSNAGLGNVHAMAHQLGGLYDLPHG
                     VCNAMLLPIVEEENAKSSPSKFRPIAEVIGMDVNNKSDKECVDFVIDKIKALSEEVGI
                     PKSLKDVGVDNPNFELLAENAMKDACAGANPVFFDKKKIIELFMKIS"
     gene            complement(16831..17700)
                     /locus_tag="L3M60_000338"
     CDS             complement(16831..17700)
                     /locus_tag="L3M60_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBN6225045.1"
                     /translation="MRGWICVKLTKLNIKKYRAPIYKYALANVNLRSAKSTNSSIITV
                     IPQGAKMEVLDEEDDWIKVMYNSQEGYVYKDLVSVSEYAWSNLNLREDKSTTSNIITV
                     IPEKSRVEVLQVDGDWSKVVYDNKTGYVFNYFLSTDGNKPNELDYKYFYTDMIKFVNE
                     NNIKSTSDYLIVTDLRNKYTYIFKKDNGGWGQLYKWQCTIGKPDTPTITGIFYISGRK
                     PSFGTDEYSVKYATRIKGGYYYHSVLYDSTGSYIIDGRLGEALSHGCIRLSTENAKWI
                     YDNIPDTTTVIIH"
     gene            complement(17816..18529)
                     /locus_tag="L3M60_000339"
     CDS             complement(17816..18529)
                     /locus_tag="L3M60_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HBN6225046.1"
                     /translation="MNKISKRGFDDSDKRWFSSKELIRLIKAKEEIEWLLNRDYKIDP
                     IVNFVGDRYQFSIRQRDALKRAVCTDEKNIIRQSKRLSLDKIKENTVNIDGFNLIISI
                     EVALSGGTLVIGSDGNIRDLAGLRGTYKIINKTEEALNLIGKFFNKYNAQNLKFYLDA
                     PVSNSGNLKYRILEHAKTWGIETEVELVKNADVVLEKLDRVVSSDAVIVDKCISYFNV
                     ARGIIEEYIKDCNIVNLNK"
     gene            complement(18898..20286)
                     /locus_tag="L3M60_000340"
     CDS             complement(18898..20286)
                     /locus_tag="L3M60_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN6225047.1"
                     /translation="MKKIFDAIIIGFGKGGKTLAGNLANRGLKVALIEKSNKMYGGTC
                     INVACIPTKSLENSANNVKTKNINSWDEVQVEYEKAIDKKETLITKLREANYNKLNSN
                     ENVTIFTGMGTFIDEKTVQVKTENKIYELVADNIFINTGSRPFIPNIKGIENKNVVYD
                     SESLMNLRTLPKKMTIIGAGFIGLEFAGIYSSFGTEVTILNSNNGILPNEDVEDSEEI
                     IKLLEKRNVKIINNANIKEIKEVSKLAIVEYEVDGKSKELASNIILVATGRKANTEGL
                     GLENAGIELNERGFIKVSETLKTNKEHIWAIGDINGGPQFTYISLDDYRIIINQLFGD
                     KTRTTSDRKNIPNSIFISPAFSRVGLNVKQAKEKGYKVLVANMPIEAIPRAKQIGKTD
                     GFIKIVIDKKSNKILGASMICENSSEIIHLIQLAVDLEVEYTYLRDRVYAHPTMTEAL
                     NDILSPSMIKEV"
     gene            complement(20407..21684)
                     /locus_tag="L3M60_000341"
     CDS             complement(20407..21684)
                     /locus_tag="L3M60_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HBN6225048.1"
                     /translation="MQNVNLFLVFIEGIVSFFSPCILPILPIYLSILSNSSVENLKEG
                     KTSFIGSSLFKNTIFFALGISTTFFILGSSVKVLSMFFNENKDLIMFIGGIIIIIMGL
                     FYMGIIKSSILNREKRFNVKFKEMRAITAFLLGFTFSFGWTPCIGPILASVLVMVSSS
                     SNHLSANLLIAVYTIGFILPFIITAMFYSKLFKTIDKIKSNMEIIKKIGGIILIVSGI
                     LMMVNGFGSISKHFNMSQNSKIESKQEENKRENSTNKEERSEGNDSQKDSNNENNDEG
                     SNDEDRIKSIDFTLTDQYGKTHKLSDYEGKVVFLNFWATWCPPCKEEMPHIEQLYKDY
                     NKNNDDVVILGVASPNLGREGSREHVVNFLKDQGYTFPVVLDEDGALAYQYGINAFPT
                     TFIIDKEGYVTQYIPGAMDKATMASFIENQRNK"
     gene            complement(21821..22156)
                     /locus_tag="L3M60_000342"
     CDS             complement(21821..22156)
                     /locus_tag="L3M60_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225049.1"
                     /translation="MYNIDDMIYNCPVEALSNILGKKWVAIIIWKIQRDKKRFGELQR
                     AIPDCSKKMLVQQLDFLIEQGIIVNQKKLVNNIVESTYFLSESGLLLLPVMEKMIMWS
                     NKNLNCDKY"
     gene            complement(22607..25207)
                     /locus_tag="L3M60_000343"
     CDS             complement(22607..25207)
                     /locus_tag="L3M60_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBN6225050.1"
                     /translation="MIKAHIVNHTHWDREWYFTSGDALVLSEQLFTDVIDELERNPDV
                     SFVLDGQLSILDDYVQLYKEKIEIIKKLIKEDRLHIGPWFTQTDAFFARGESILRNLM
                     IGIFESKKYGKYMKIGYLPDTFGFNAQMPILLKHVGLDNIFFWRGIHLGKQVQSPYFK
                     WKSLNGESYIYAVNMPHGYGTGMLLEPSSKYVNGRLDPAIDFIKSYTDVDEVLIPSGN
                     DQLNIIGDFKNKVKAINDIGKYSYITSSYQEFLKYIKSIESLESYRGEFREPVLARMH
                     KSIGSSRMDIKLACDKLENKLIKRIEPLLVIAKKSKIEISNQLLLNTWKKLLEGQAHD
                     SLAGCVTDTVTDDILHRIREANEICDSIENTIVKKISERLKLSKNDILVFNTEAKRFN
                     GYKEIQVVSDSKNIYFKDNLDAIIIEETYVKPRENVLEETPAGNVFIEEPGYYILKVR
                     INIQLPALGYKVVSFESSDKEMVSLNKSNDSFISNDSYKVIYEDGSLNLQLKDGTYIS
                     NFLTLKDSGNAGDTYDFSPLKNDEDIKLSFDRAYTEKALGYEKLVVKGTTLLPLTLED
                     RKNKYLNGKLNVKVIIILSKENPLMDVKLCINNTIYNHRLRVHIKTDIKDNKNIASLP
                     FGYITRENGVLDNWEDIYSEMPIDLEPLESNITLTNQNRSCTVFTRGIKEYQHIKDEI
                     ALTLLATTDELGKPDLLYRPGRASGDTTKKGHIRIKTDKAQIFKKLEFSFAIYMDSKS
                     FDELEIANFTHNYLRECVNYQLQDYNFFLYRIDNKIQKSIINKTVSRELEIISLPENY
                     LISACYPSYYSKDKFVIRLENQSSKRMILDEQIFKNINGKIVNAIEDVEEEQLYEIAP
                     FDVISILLDL"
     gene            complement(25237..26352)
                     /locus_tag="L3M60_000344"
     CDS             complement(25237..26352)
                     /locus_tag="L3M60_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBN6225051.1"
                     /translation="MEGNVMLKKIVSELKKHVLTGISYMIPLVIAGAMIMAISRVGGS
                     FYNIPDIWDAKYAESASSIVRLLHDLDGFGGTALGLMFPVIAAFIGFSIADKLAIVPG
                     LVGGMIAKDIGAGFLGALAVGLIAGYTCLFIKNHVKLPKSAASIVPIFIVPVFGTLIT
                     VVLINYVIGIPFATLNTGLENWLNGLSGTNQILMAAIIGAMMAVDLGGPVNKAALTTS
                     LALLTSGIYAPNTAAMVGIVIPPLGLGLATILAKQKYNKQLREAGKSSLIMGLIGVSE
                     GAIPFAVESPLKVIPSCVIGTAIASAMAVGLGSVNATPISGFYGWFTVENWPMYVLSI
                     VVGTVIVAGLVIVLRGNPVIEDDEFEDDDFDESEWEA"
     gene            complement(26353..26673)
                     /locus_tag="L3M60_000345"
     CDS             complement(26353..26673)
                     /locus_tag="L3M60_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBN6225052.1"
                     /translation="MKRKIIAVTACATGVAHTYMAAQALKKASKKMGHLIKVETQGAT
                     GIDNELTTKDAQIAEVVIFAVDTKVRNAERFEGKEILEVPVNAPIKDAEKVINDALKL
                     IDTK"
     gene            complement(26676..27137)
                     /locus_tag="L3M60_000346"
     CDS             complement(26676..27137)
                     /locus_tag="L3M60_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225053.1"
                     /translation="MKISDVLKKEQIVFNLDTNTKEETIKKLSSIFVENGIVDNEEEY
                     VKSVLEREEHSTTGIGNGIAIPHGKSNAVKKSSIIFAKTKHRIEWDALDEKPVDSIFL
                     LAISNIDKDSNHLVLLSKLATKLMDDDNVDALKLANSEQEIINIFSEEGEM"
     gene            complement(27137..29101)
                     /locus_tag="L3M60_000347"
     CDS             complement(27137..29101)
                     /locus_tag="L3M60_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6225054.1"
                     /translation="MLTDMIQNREIKIIKFLLKEGHTTVKAIASDLDVSEKTVSKSLK
                     EVEAVLKKIGLSLVRKPKIGVYIDGDLKKILPYLDNKGSQIPTTREERVIYIYSVLLK
                     SNDYITIQQLSDELYISRGTIERDLAEVEKLLKNEGISIYKKPSKGIKLNISERDKRQ
                     LTAKFIHKFWSRNWYIKQEQESFYQAFEKIQADVNGIFSEDDLSIIIEILRNFSRERK
                     FEFSDYEFQSLVIHLAIAVERIRKEEYIEESLYIGNIENNQMSNTNYLAKQLEEKFSI
                     LLPKSEIGYINLHLVAADQHRDTNELKNTSTNPCETNTNNVSNLREIIKNTLYETAYD
                     KDLIDGLTSHVHSSINRLKYGLSIKNPYVNTIKQNFSLSFEQALDLKKVIEFIYDIRI
                     NDNECAYIALHFEAFRERMKELPQNIRVILVCSTGLGSSRLLSARIKKYFPMIKIEKV
                     LSVQELVSSKIDADLVISTIYLELETIPTIVVSPILNESDIRTLERNIQNVSGCTSKK
                     YKSFRNLIFEENILLNIEASNYEDAIKYITDNLIKKCFAKEGIAKSAIERENLSYTSF
                     QSIATPHANPSYIKKSNISVATLKNPITWGNEKVEVIFFISLENDKNLELDEIYEVFY
                     DFIDNKKNINDILSATSTKEVYDLLVEESI"
     gene            29267..30121
                     /locus_tag="L3M60_000348"
     CDS             29267..30121
                     /locus_tag="L3M60_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009912760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar uptake protein"
                     /protein_id="HBN6225055.1"
                     /translation="MLTIIIALLPVMGWGLMPIVANLKKSSPHEQLLGTSISAFIFST
                     IITLVIHPEITFFSFCISMVSGIFWSLGQLMQFKAIQIASVSKVMPISNGSQLTFTTL
                     LAVILFNEWTSSKMLLIGSLSILCIILGILLTNYQTKKSTDKNMLKCVGVVLLSSLFL
                     SLYVVTNQIFLIEGYRIILPQSVGMLITSSIIVKYFSKEIVSRENVLFNLITGILWSI
                     ANLGMFITTNTLGVTISFSISQSCVIVATLGGIIFFKENKNKKEWFSILIGIMLIMSG
                     VFMLSIIK"
     gene            complement(30363..30830)
                     /locus_tag="L3M60_000349"
     CDS             complement(30363..30830)
                     /locus_tag="L3M60_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C_GCAxxG_C_C family protein"
                     /protein_id="HBN6225056.1"
                     /translation="MKTKEEYLKEVRTTAEECFRRGEFFCSEAVLHTINNALGQPLSP
                     EITKLASGFPVGLGKAQCLCGAISGGEMALGLVYGRVHGEAMNPKMFEHAKGLHDYIK
                     KEYGATCCRVITKKWDGDNFMSPERKEHCIKITGQVAEWVADKLIEDEKLNIK"
     gene            complement(30934..31866)
                     /locus_tag="L3M60_000350"
     CDS             complement(30934..31866)
                     /locus_tag="L3M60_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HBN6225057.1"
                     /translation="MNLLKKYPIPIASLILSIFTLGNLLQSYSESLRNVIGVIGFILY
                     AIYIIKIVLLRKGVKEQLENPLISSTFPTITMATMIFATYVKPLSLELGIMIWSIGVV
                     GHLVLIVLFSKKFLIKFSIKTVFPSWYIVYVGIVAASVTAPAFNQLLIGKIAFWIGFV
                     SYIVLIPVILKRVWIIKEMPEPSLPSIAILAAPGSLLLSGYINSFSDKNNVVLYLLFI
                     LSIVFYIIVLGYLPKLLRLPFYPSFAAFTFPMAISAMGMKLINGHLTKSGHFISWISY
                     ASKLEEIIATVIILYVLVLFIIHLSKSETTSKAK"
     gene            complement(32035..32979)
                     /locus_tag="L3M60_000351"
     CDS             complement(32035..32979)
                     /locus_tag="L3M60_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HBN6225058.1"
                     /translation="MIVSVSRRTDIPAFYSKWFFNRLKEGFVYVINPMNPKQVSKIEL
                     NPHTVDCFVFWTKDVTPMICDLNKLKDYKYYFHYTITSYGKEVETCILDKRKVIDSFK
                     ELARKIGKERVILRYDPIFLSEKYTVDYHIKAFASLCNQLDGFTEKCIISFIDLYKKT
                     KFNTKALHIKPIEITEIETLSKEISKIAHKHGITLEMCSEEYNLSKFGIGKSKCIDDR
                     LISKIIGCEVEVKKDSTQRDICGCVKSVDIGQYNTCRHYCLYCYANFNYAKVEENCRL
                     YKEDNKLLIDTLRGDAKISIRDMKTIKVDKNKQISIFD"
     gene            complement(33619..34293)
                     /locus_tag="L3M60_000352"
     CDS             complement(33619..34293)
                     /locus_tag="L3M60_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6225059.1"
                     /translation="MNDELKTILYSKGVDIVRFVDISEFPTSQTQGFSKAILFCIGLS
                     KKFITDIYNNLPTGSDEFLEKEEKVEGLADWISKYIQSKGYRAYSQSEKNNLEHGYFE
                     KAYINPEMQSGISPLPHKTIANISGIGFMGKNNLFVTEEYGCAFSMCTVLTDAPISVE
                     RHPLIDSKCLDCNICVENCPAKAIHGNEWTLPGERESIIDVSKCFCVLRCMMSCPWSL
                     RYANQK"
     gene            complement(34353..35099)
                     /locus_tag="L3M60_000353"
     CDS             complement(34353..35099)
                     /locus_tag="L3M60_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HBN6225060.1"
                     /translation="MKIENLSINDIKRGYIFNDDLNCYSCIKCTKIYEIGEIYKIGNR
                     FFEASKAIELHMELEHSDYLEQMINSDSKYNTLTENQKQLFQLFISDLPDKEIAKKVG
                     VSTSTIRHQKFMFREKAKQAKLYLALYESVFEDKSNKGSTIISIHEHARMVDERYVVT
                     EEERKHILDTSFESMNPLKLKVFSSKEKKKVVILSKILEQFERGKQYSEKEVNQILES
                     IYGDFATIRRYLIEYGFMSRNKECTKYWLT"
     gene            complement(35577..36263)
                     /locus_tag="L3M60_000354"
     CDS             complement(35577..36263)
                     /locus_tag="L3M60_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225061.1"
                     /translation="MAILELINVNYKYEGSSKFVLKDVNAEFEIGKMYVIVGKNRAGK
                     STLLSVISGLDDCTSGSIIYKGEDLKNINKNIYRSKYIGSIFKNHNLLNDATVLDNIV
                     LTMLIAGNKAKNKEDIVYDILKKVGIDKEKANCKVKDLSQDDIRRVCIARALSNNPEL
                     IICDDIIENTDDKGEKSLVDIFYKLASEDNKCVIITTTFDRIALYADELWGITNGNLM
                     FVKSLEKLKY"
     gene            complement(36274..37632)
                     /locus_tag="L3M60_000355"
     CDS             complement(36274..37632)
                     /locus_tag="L3M60_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225062.1"
                     /translation="MYILNNAIKNLIRNKVHSLFSIVIILLMVLSIAISSIINLNMSI
                     ITQKYEKKLESEVSIKIDEKKVVQNDLTESLIGYDPLNLEDYLNFSKSKYVKKMSLTG
                     NLALYVDNKKYAPSNDKKLDYNDITTNCIVMGFTDKNKSLKYYNLNISKGRVFQKDNE
                     CIVSEDFLKAGNFKVGDTISLLNDYNTNVKLNLNIVGVYKTKDSNKLESVQDVSIYNN
                     ILTSYNTLVTFEKRVRPESKMIYTSASFLLRDSNSRKAFEKELRNKGLSDIYKVTIDE
                     LLYHKLMAPIERLSGVVFTYTLGILVLGSILLVISSAISIKKIKYDICVLRLIGMSKF
                     KIIRSFIYESIMIILISTVLGLGIASISEKSISNYVFEYQYKYNITYNDFLFNKGSYK
                     DVNTNDINTEQEMKNLKDKSTLNFILKIIFISMILILLTSTVSIYNAIMCKPIQIIYD
                     RN"
     gene            complement(37694..38740)
                     /locus_tag="L3M60_000356"
     CDS             complement(37694..38740)
                     /locus_tag="L3M60_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225063.1"
                     /translation="MQGLKDKKTIALILYSVIVFIIFTVGLKYIINISDIDKNLISRD
                     TTLSKSEIPLNNSYVLDKGLMTNYNLILKNNFNVVIILFFAFIVTSNFILLLILNRLD
                     FKERIEILNNINNIEDENEILSIDPIMSRVYKDLKNKFDSHIEDYKRLNSYLSHEQKN
                     AISILRTSLELDGNKELLNVLDKVSNSVEDVLAISYIKNNEDMYEVDIALICAQVCDN
                     YSKVYSNLKFDFDEDENMSIFGREKWIYRGVSNLLDNAIKYGEGKEIYINVKNQKGSV
                     IVSVKDNGIGMNQHHIDKIFYDRYRINELNKDGYGIGLSLVKHVCNLCNGFIWVESEE
                     NKGSTFYLVFKEYK"
     gene            complement(38728..39399)
                     /locus_tag="L3M60_000357"
     CDS             complement(38728..39399)
                     /locus_tag="L3M60_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225064.1"
                     /translation="MRVLIIEDNKELSYNIKSGLENAGFVVDIANCGLDGEEKAFVND
                     YDAILLDLNLPDKDGIEILEFFRSNNLNTPVIIVTARDGVNELAKGLDLGADDYIVKP
                     FQIIELRARIQAVIRRFYGRTNPNIESGPITINPSSRKVYVYNEEVTLGTKEFDIVEY
                     LASRYPDIVSGIDISEHVYDEYFDPLSSVLRVHIARLKKKLREVAGKEIIITTRGIGY
                     SICKD"
     gene            complement(39786..40670)
                     /locus_tag="L3M60_000358"
     CDS             complement(39786..40670)
                     /locus_tag="L3M60_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN6225065.1"
                     /translation="MRYDIAIIGSGPAGLAAAINAKIRNKNIILFGTEDISQKLIKAP
                     SVENYLGISNISGLELKEKFKNHIDDMGIKITQNRINNVYSMGEYFTLVSGNHVYEAT
                     TVVLATGIEYGKMIKGEDAYLGKGVGYCATCDAMLYRNKKVAIIGYNYEAEEEANFLS
                     EIASNVYYIPMYKLENKINSGINVIDDKPLEIKGGKLVNKLVLRKSELDIDGVFIIKD
                     SISPKYMVPGLEVEGSHIKSDKNMNTNIEGLYVAGDCAGKPYQYLKSAGQGQTAVSSA
                     ISYLDKQKIKEFMLVDAH"
     gene            complement(40836..41153)
                     /gene="trxA"
                     /locus_tag="L3M60_000359"
     CDS             complement(40836..41153)
                     /gene="trxA"
                     /locus_tag="L3M60_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBN6225066.1"
                     /translation="MIKIINHNEFINEVENKDGLVVVDFFATWCGPCKMLSPIYEALG
                     DEMAEKAKFLKVDIDQSIELAQKFEVSTVPTMMIFKDGKPVDRLIGFMPKDNLKNKVE
                     SYM"
     gene            41405..42157
                     /locus_tag="L3M60_000360"
     CDS             41405..42157
                     /locus_tag="L3M60_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HBN6225067.1"
                     /translation="MNDIINELQNIGFTKYESQIYISLLKESPLTGYEISKLSGVPQS
                     KVYENITKLLNNNIIINVGTDPIKYVPISPNELLKNKKEEFNNSMDKLKNNLQILNKG
                     KNIEYVLNIKGDKNILKKAIEMIKNAKQEIIISCPYEEILELKKELLIAYKKNINIEI
                     LLLENKNLEGLDNIHIHGGNKTTLENKMLYKGIILIVDNNEILTGNLSEGNEAISIWT
                     KNSNILYIGVQYIKHEIYISEQIKGGYKNEQD"
     gene            42144..43025
                     /locus_tag="L3M60_000361"
     CDS             42144..43025
                     /locus_tag="L3M60_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HBN6225068.1"
                     /translation="MNKIKIKQVDAFTTIPFGGNPAGVVTNASNISDEIKQNVAKEMN
                     LSETAFVSESNVADFKVQFFTPGLEVDLCGHATIGTFHALLEEGKLDSNKSIFYQETK
                     AGVLSVELKEINNENIFMMEQNTPEFENLDNYRKELAQMIGVHEDRLLEYPIMKVNTG
                     LWWLVFGLKSLDDLKNISPDLNKIKKFSGDNNIIGITPFCIETIDKKCDYHLRSIAPY
                     VGVTEDPVCGTGNACVSSYIVNQNILDKTSFIGEEGNFINRPGKVYVNIAKENNEITS
                     VKIGGSAYTVLSGEIIY"
     gene            complement(43252..44607)
                     /locus_tag="L3M60_000362"
     CDS             complement(43252..44607)
                     /locus_tag="L3M60_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBN6225069.1"
                     /translation="MSSTVKKKGKFPMGFYICSTTFSFERAAYYSAKFLIYIFLTTAV
                     IHGGLGIDKGQAAIMQANLVAFTYLAPIIGGYISDRWIGARYTIPIGMLIMGVGYYLG
                     SIATTVSMVNAMIILVSIGTAFFKGNVSAVNGQLFDNQEELDTAFSVQYSFVNIGSFI
                     GTTAVGILYAKTFAKNGVLGFSQCFFIAAVLCVIGAIWFTYGWRFLGNAGKRPFKEGV
                     VAEKVEEKDKSPLTSMDKKRIWAIILISFFSVIFWVFWYLTYLAVYDYGAAFVDMTIG
                     GFDIPLAWFDSLNALVCIVLGPVLGALWFKLASRPQGDMSLFKKTGLGLIFLGLAFLM
                     LVGAEFTRGVGAPETAKASIMWIIMFGILLSFGEMLFSPLGNSFVSKYAPKKLLGVLM
                     GVWTFATFIAGQGYGYIYAFTLKFEMIKVYTIIPVILFVAAILLFLFDKTLNRLVEDD
                     K"
     gene            complement(46469..46978)
                     /locus_tag="L3M60_000363"
     CDS             complement(46469..46978)
                     /locus_tag="L3M60_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBN6225070.1"
                     /translation="MVMFLFKIDDYIMYGMTGVCKVMDITNERFTNGIKKEYYVLNPI
                     YSNNTIIKIPVDNEKVPMRKLLSKINVLSLINDIPNMDTSWIDNEKLRSEQFKKILRG
                     GKCEELIKLVRSIDNNREYVKSIGKKTHQADDNIMKEAERLLNEEFATILDISPNEVS
                     SYISSHIPQ"
     gene            complement(47189..48598)
                     /locus_tag="L3M60_000364"
     CDS             complement(47189..48598)
                     /locus_tag="L3M60_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225071.1"
                     /translation="MENYFDKQAKELYDKASEIINTHSMLKANRANEKFDIDIPQDFK
                     YEFFSLVDKVNLSLMEEEDNFYGYFLFQMLREIRFDISSPTGVNFKGAKYVIYFNPII
                     FLTLDIKQMETTIKHEIHHILSMHLMRAKELKGKYSTLAINMAMDIVVNKFLNNLPPY
                     ATTLEWVNLKYSLKLEPYEPFEYYVEKIQTELDLMEVDEDGEEDDSDKYGDIEIDYSP
                     ERTHDIWDESNEIDENTLREFTEKFINSSQKGKVPAYLESMISSLKNSKSELPWNLYL
                     NRMMGTVENNKKKTITRRNRRQPNRLDLRGELRSHKAEIAVALDISGSISDEEFKQAI
                     KEVLSIVKNYNHEIIIIECDNEIRRVYKVKTEKDIKDRIRIGGGTRFSPVFEYANNKK
                     INLLVYFTDGKGENKLQVIPRGYKILWVISGRGDKISLKEPYGAVKKLTKVETNDEIL
                     EINDVRNDGYSMNSQAPIL"
     gene            complement(48602..49732)
                     /locus_tag="L3M60_000365"
     CDS             complement(48602..49732)
                     /locus_tag="L3M60_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6225072.1"
                     /translation="MNFIDTLKSVDLILSTGEVPLIVGESGIGKTALANKLAKENDWS
                     LVAIDGNLLKEGEIGGLPTIESYVGVNSSGYKTEKKTTVYAVHNKLREIDEEISKGKI
                     VLLFIDEINRCEHTVQQELMNLILNREINGYKLHDDVKILAAMNPSSKYGSDFDYQVV
                     DMDAAQENRFVWLNMESDHTQWIKWAIDEGIERKVIEFISTFPEYLHKINEDDVRATP
                     RSYERVSKIYKVYKENNNSIPRAVFLNVIKGNVGKVIAEEFISFIESDSKPLISYEEV
                     FLGESIDESIVERVKKESHTRLYLSAMNILKDLELNIKNDKYESNHYINRFIKFLKMY
                     PVDLMIGIMKDIRNSYTEVYKKAIENEEFVKSYFESYSLIRG"
     gene            complement(49744..50547)
                     /locus_tag="L3M60_000366"
     CDS             complement(49744..50547)
                     /locus_tag="L3M60_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225073.1"
                     /translation="MEKSRVIEMIKNNPNIISTIDNPTDEMKLLAIKENGLVLEYINN
                     PTREMQELAIDNNIRAIKFIDNPTEEMMIKAVNEGWSILDYIKNPTDKVIEMAINQAG
                     WAIKYVKNPSEELQLLAVRKNYDSIKFIKQPYESVQEEAVRISYDALRYIDSPSYSAE
                     LIAIKNNEAAISFITDLDKNKMLEFLKVNILVIKYIVKKISKDELEEVLKEVLSKEDV
                     EEKYIRDFLNCSVIDRHSENIPIDKIMFIYKYGSKTAKKIAVDEKLKIV"
     gene            complement(50961..53168)
                     /locus_tag="L3M60_000367"
     CDS             complement(50961..53168)
                     /locus_tag="L3M60_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HBN6225074.1"
                     /translation="MYSKIGGINLKKIKKKRIALLLATITTLTFNQKFLVHAYENNYN
                     SEEKLWLPDFTDNDRAKQLEISKLTEKVNLNSIALERIKNINSKQIIENVSPAKLGDE
                     IVKQGDTSNFSLEDNSENIKLPSNVDNSQTKYFPNIGSQGSLASCASYATAYYTMTYM
                     TALVNDWSVKDVSGKNIDSKIFSPKWSYNFINDGKDAGAFAYQNYNVFKTHGCATLAD
                     FPYVGLGRDPKNYREWAKDENIWRNATKYQVDEILRVNFNKNNKPNSFITSNKDSNLD
                     DAKRLLLNGYILNFYTWFSDANYKQISNDPSTKDDDSYVNQKAIYQINNQGAHEMTIV
                     GYNDNIWIDINSNGKVDDGEKGAFKIANSHGPDYANNGFVWMAYDALNKKSSVLDFTN
                     SNARQGAFLNNEAEYVVISKKETPSKLVRFTMQHDNRKQLQVSLVYEKNGNKQTVVYS
                     PLLSYSGGDFPLDGVNNGSEITFVIDLAHLEKELNVSLSSADKIYLQIKDNLKDDKVA
                     IIKSIILVDNKGHKLSPELISKNVEINGDSKMFDLGQIDNSSELYLQSPEISTDKTLS
                     NNGKFNINVSVPPNNSATKLELFENNVPIETKDLVRSSELQNINFDMSDKKEGFYTYF
                     AVLEDGNGKCLKSEEVNIIVDSIIPPSIKISPNPNKVDVEVIVPAMNNAKTLNVYMDG
                     QKIVNEELSVRNPNEVKKSFVLYSLIVGTHTFWAEVEDENATISSQKVSITID"
BASE COUNT        17744 a         8996 c         5552 g        20929 t
ORIGIN      
        1 catagctatt ttttaatcaa atttaatttt aaaactgcat caaaaaatga agtctcaaca
       61 gacttcactt tttataactc ttataaatta ttcaaaatat ttatacttcc ctttctactt
      121 caaaacgtaa aaccgtcttc aatgattcgg gcgatacttt tctagcatca cttatataaa
      181 tctcatgatg tatgtaagat tttcttataa gtttattttt tttacaaaat tcgttcataa
      241 tatcaaagct ttctggttca ttatcatatg aacctatatg aagcatttga acacatagac
      301 cttcattaat agacccaaat ctaacttttt caagaagatt atgaggtttc ttcttcttta
      361 ctatatcagt agctttatcc aacaggtctt gtgtaacaaa atctggctga tgtatcatta
      421 gagtataaat taaatcttct ttaataagta catctaaaca tttgcttttt tctcctttat
      481 cccatatgcc ttctaaagga aaaacagtat agtcaaaata cccttctggg acaattccct
      541 ttttaggcat ggtttttatt gcatatgcca aagaataaag tactcctatt gcttcagtaa
      601 attctttaga atttgggtct ccattccctt ttaacatgat aaatttctgt tctggtactt
      661 caattattgc aggattcttt ttaggtaaat atagctcttt atctcttttt ctccattcat
      721 atttcataaa aaatacctcc acatatattt catacattga tattatcatg ctaaaagtga
      781 caacattatg tcataattat aaattatatt ataaaatctt catcctctaa ttcctcatac
      841 actccttttt ggtatccatt acccttagtc gaaaagaaat catgtgtttt agtttcagtg
      901 ctaagaccat ttaatacaat tggatttata gcatctacag tatataactt ctcaaaccct
      961 agattttcta aggctctatt cgcattgtat tttaaaaaat tcacaacctc tttttcaaga
     1021 ccactttctc tgtatataat atttgtatac tctatctcat tctccataag tctataaagt
     1081 atagaataca tcttctcttc taatatctcc ttatccattt tatcaaatga atcgtatatt
     1141 tcttgtgcaa gcaatcctat gtacttacca tgtaaagact catctctaag aataagcgat
     1201 attatttctc cactagctac catctttcct tgaccagata gatacaatgg ataaaagaat
     1261 cctgaataaa acaagaaact ctctaaaaat acacttgtaa ccattgataa gtataatccc
     1321 tcttgatttg tagtattttc gtattgagct aaaactaaat ctgcctttct ttgtaatgtt
     1381 ggttctgttt ctatccattc aaatagttca tctatttccg aattagaaag tagagtcata
     1441 aatatagaag aataactttt tgcatgtatt tcttccatag tccccataaa agacaagaca
     1501 gcttttcttt gtaagttttc tgttaaactc atcatagatg gtattccatt attcccctgt
     1561 tttgtatcta ataatgttaa accacccaat acttttttat ataattcctt ttctttatta
     1621 cttagttcat tccatatctt tatgtcttta gaaacagata tttcttcagg aagccaaaat
     1681 tgttttacat tttgttccca aaaagcctgt gtaaatccat cttcctctct attccagtta
     1741 actgcattat gtattttatg taaattaaat gtcatatatt tctctcccta tactgaacac
     1801 actagacact catgagtgtc tcttgtcatc tttgttcttg tgtaatataa acttttcaaa
     1861 ccttttttat aggcatatat gtaatgtcta gctatatctc tagttgtctt ttcatctgta
     1921 acaaatagtg ttgttgatat accttggtca acatgatttt gaactgttgc cactaaatct
     1981 ataacctttc tcatatccat cctatatgct gattgataat acaatatatt atcatttgtt
     2041 aaaaatggca ttggatatat tgtagtagaa tctccatatg tccttacttc tactggctca
     2101 gtaattggca ttatacttga tgtagaattt tgaacataac taatgctttg ggttggtgct
     2161 atagccatta aatatgcatt atatatccct ttttctttta cctcatctaa taattttgtc
     2221 cagtcttctt ttgttggtat ataaatccct tcaaataacg cttttacctt ttccgactta
     2281 ggcagataag attgttcata gtattttgat aaaaccttgc tatttcttcc cttagcatat
     2341 tctgatttat caaacccttc aaaagtttga tttttttcta tagctatttt catagaagct
     2401 ttaattgaat agtatctaac cattgcaaaa aatacattag aaaaatctat agcatcatct
     2461 gaagtgtata aaatattttc tcttactaag tatccatgta aattcatagc tcctagtcct
     2521 atggagtgag acttgctgtt actgttgctt actgttggca ctggttttat atctgtgcta
     2581 tctgctacaa atgacaatgc tcttattgct gtttcaactg tactttctat agttttatta
     2641 tccatcacat tggctatatt taaagaaccc agattacaac ttatatcttg tccccattca
     2701 tttttgcctc catacccctc tatttctgaa tgtgtttgat attgaaatat ctcacaacat
     2761 aagttagaca tcttgataat tccaatatct tttaatgtat gttctctatt agctgtatct
     2821 atgtacacta catatggata tccactttct tgttgcatct gtgctatttt tgtcaacatc
     2881 tgcctaggat ttatttcttt ttttcttata tcattatcat taaccaattt atcataccac
     2941 ttattcattt ctatctcatc aagagaaacg ccatatttct tatacacagt atgaggataa
     3001 aatgcatatg ctacttcatt tttttctgcc aactccataa atttatctgg gattatagct
     3061 cctaatgata atgtagcaag ccttattttt tcatctgcat ttatcttttt agtatccatc
     3121 aataattcaa aatcaggatg gaatacagta aggtacactg caccagaacc ttgtctagct
     3181 cccatttgat tgaaataact aaatgattgt tctaacattt tagctactcc aacaacacct
     3241 ccagcagcac cttctatatc tttgatagat tctccagatg ctctaagttt agataaattc
     3301 agtgcaactc ctccaccaat ctttgataag tgatttgatg aagatatagc atacgatata
     3361 ccttctgtac tatcttctac agatagtaaa aaacatgata ccatttctcc agctcttttt
     3421 ctcccagcat ttaaaaaagt aggtgtagct ggttgaaatt cttgttttac tattttatct
     3481 gccaagttta aaactaagtc tttattgcca tttcccaatg tcaatgctac aattaacact
     3541 ttatcattat atgtttctaa tattttcttt ccatcattgc tctttaatgc ataattttga
     3601 taaaacttat ttgcactcat ataagattga aattcaaact tctgtttatc aataatctca
     3661 tatatatttt taataaagtc catgctatac tgatttatta cttcttttga ataataacct
     3721 tcatctatta aatacttcaa tctttcttct aggttattaa attttcttaa ctttggttgt
     3781 ataaattctt ctatgtaact acttaaggct tccctatcct tttccaactg atacttacct
     3841 tgttcatttt taattatcac ttgattgtta agctctatcc aattcattat ttaccgtcct
     3901 tttaacataa tcttgtgcta taatttcatt taatttgctt attatttctt tttgtactta
     3961 ttagttgaaa tcccaaaact aatctataat tataggatat aataagtatt tttcaaaaac
     4021 aaaagcacta catatagtca gtgctttatc tattatatac tacatatagt atatacacaa
     4081 atttttttat atctaaactt ttattattgt atacctgaca ttatttttgt ttaaagaaat
     4141 ttttatcaac tcaaatataa aacaatataa gcttattatg atatatttaa attttcaaat
     4201 catctctaaa tagcctaaaa atatattata ctatattgaa aaaaattatt ttaaagatga
     4261 tattttatat attatttaga agttttttac ttattgttat ataattaaat atatgactat
     4321 tttgctacta catatataaa aataaatttg tgaaggtgat tttaatgaaa actatttcta
     4381 aacaagaatt agaaaaaaca cttgagaaac attatttatg gcttaaaaac aataatactg
     4441 gagaaaaggc agatttaaaa gaattaagat tggaaaacat ggatttaaga ggatactgct
     4501 taagtaatat tgacttttcg tggtctgacc ttatcaattt aaatttagaa aaagcagatt
     4561 tagaaaactc tatttttaat aattcttatc tttcggattg ttcattaaaa aattctatat
     4621 taaaaaatgc agacttaagt ggtgcaaatt ttagattttg tgacttatct aattcagata
     4681 ttagaggagc taatttagaa aattctaatt tagaatatgc tactttaaat ggagtaatat
     4741 cagataaaag tactcgtttt tttaaattgt actgtcctga aacaggagca tttataggct
     4801 ataaaaaatg ttttaatttt agaatggtac aattattaat acctagcgac gcaaaaagag
     4861 tatctgctac atcaaatgct tgtagatgtg ataaggcaaa agtactatca attactagta
     4921 tagatggtaa agaatctttt aaaagcgctc gtgcatatgc tgatgaaaat tttatatata
     4981 gagtaggaga aacgattgta gctaaagatt ttaatgaaaa tagatggaaa gaatcaacca
     5041 cagggattca ctttttttta actagagagg aagctattgg ttatctgtaa agtaacttat
     5101 ttttataacg acacatataa aatacataaa tgataaaaaa aggtctgtct caaaatagag
     5161 ataagccttt tttcattgtt gaaataatga aaatatattt aatttcatac aaaaagagtg
     5221 cctcatgaac taaaaagtcc attttgagcc actcatcttt ttatattttg taatttactg
     5281 gtatatttga ttttaaaggt tatagtaaat tattcatatt aatactatct acgatttgag
     5341 aaactttatc tacatgttct atcttgttat ccaagctgtc ataatacttc ttactctcat
     5401 ctctatttct aaaagaataa acttttttat ccaattttaa agcctctttt acaatgtcta
     5461 tattcctctt gtttgtttct cctacactaa atcctgtatc tattataatc ttagaagcat
     5521 ttaaatttct aatagccaaa tcgaaactct catcacttat cgactcaaat ggattttctg
     5581 taaacatttt tatccccata gttctaccaa tttcataatc aatatcattt tcatgtatta
     5641 ttccagaata tagacctata cctttcttag taaaagctct ataaattggt gtcgctttac
     5701 ctccaccacc aattataaaa acttcatttt tagatttatt actaatttct actgaaccca
     5761 aaagactatt aaaattttta tcatctaatt cataaagtga atttataata ttctcatcaa
     5821 ctacatcttc aggctgacca taagctatga ctttattatt ttttataaga gcaaccttat
     5881 cacaactttt taatgctatg tctatttcat gcaatgaaag tattactgat attgactttt
     5941 ctttacttaa atttttcagt atatttatga gttctaatct atgttttata tccaagtgag
     6001 ttgtaggctc atccaatatg attatttctg gttcttgaac gagagctctt gcaaccaata
     6061 ccttttgttt ttctccatca cttagttcat caaaatatct tttctttaaa tgtaaggcat
     6121 ctacagactc caatgcttca tctaccatta ttaaatcttt cttagataaa ttaccaaaaa
     6181 atcctgtata tggatacctt cctatattga ctatatcttg tacagtcatt aaatcagcta
     6241 ataatctatt tgtaagtact aaagccattt tttttgataa tgcttttctt gatatatgat
     6301 ttatgtttac accgttcaaa tatatctcac cttttattgg tgatataagc ttagataagg
     6361 acctcaatag agtagttttt ccagctccat tacttcccaa aagacatagt atttctccac
     6421 tttttacttc tatatttata tcacttacta ctaccttatt attgtatcct acacttaaat
     6481 tattagtttt caacatgtct aagccctatc cttccttttc atcaatagca tcactacaat
     6541 tggagctcct aataaagacg ttatcgtact tattggtaat tctactggcg ataaaactac
     6601 tcttgatatt aaatcacaaa gagctgtaac tatactccct aaaaatatta ctactggtat
     6661 caaaattcta ttatctgatg ttttaaatat caattttgaa acttgtggca ctgcaagtcc
     6721 aataaatcct ataggccctg caaatgctga tactattgct gccaatatgc tagatataaa
     6781 tactattaaa actctaaaaa attttatatt tataccaata ctttttgcat actcttctcc
     6841 taataagaaa gcatttaaag gttttattat gaatacagtc atcaaaagac ctaacacacc
     6901 aactgttata agtatttcta ccttttccca tgtaaatcca gaaaaacttc ccatagacca
     6961 catagtaaat tcctttaatt tatcactatt tgcaaatgat tgcaacacat ttgttatacc
     7021 actacacaca tatccagtca taattcctat tattaaaaga gttgttatat ttcttacctt
     7081 atttgcaaat agaatgacta tagacattac aattaaagct cctataaatg ctgctacaaa
     7141 tatactcatt gaatttacac tctcaaagcc caatgtgtat cctcccaaaa tcatgagtgc
     7201 tactgataaa gtagagcctg aagatacccc taatatatat ggttcaacta ttgggttttt
     7261 gaaaaatatt tgtaataaaa tacccgatac tgctaaacat gacccaccta ccatggttgc
     7321 aagtactcta gggattctta tattgtatat tattttataa tgttctgtat tatccattcc
     7381 ccttaataca acatctatta cttctttaaa ttcaattttt acagaaccaa atgctacaca
     7441 cactaaaaaa gtaagtataa tacttataat tagcactata aatattgtag tattccttat
     7501 tctcatttta ttaataaaat ccatttgtat actcctctta gcaaagctat attaactaaa
     7561 atctataaaa ccaatctttt tttgtccttt ctgtactatt tatcataatt tatataatgt
     7621 ttgatttttt cacctgtagt acctgggtga aacatctcaa ctaaatctac tattaattcg
     7681 tccgtcttat ctactgattg ataataatct ggagcaaaca cccataagtt tccatttttt
     7741 acaacatcca aatcagccaa tacaggtgca ctagatttta tagaatctaa gtttggtgca
     7801 taatctgaac ttgatgagta tacaaatata tctgcatcct ttgcaacttc atgcaattgt
     7861 tcaatactca ctcgtccttc atttgaatct attgaagcac atgcatttat tccaccagcc
     7921 atttttatca tattatccac atatgaatcc ttaggcgcta tataagcact tccttcatat
     7981 acagatgccc atacaacttt aggtttagct ttgtttttta tttttttact tacttcttct
     8041 atcttttgta cagttttatt tagttcacta gtagctatat cttctttatc ataaaatgca
     8101 gctattaaac ttgtccactc cattcttcca aatggatttt gctctttata ctcattgtct
     8161 acaacataat taagacctaa ttcttttaat ttgtcaataa attttgtatc agcctctgat
     8221 aagccactac tcactatagt taagtcagga tttaaatcta ctattttttc ataatctggt
     8281 gctaaatttg aaccaacata atgtattttc ccatctttca ttaaatcttt aatttcttta
     8341 atagtccaat actgctcttc tgttgttact gcttttatac tatctagttc atctattgtt
     8401 ctcagagaac aagcctgtgt tgtggatgct attaatacat tatccacagg cgttctaact
     8461 atattagcgt ttttatattt ttcaggaatt ttaattttct ttggtattaa aatcagttct
     8521 cgattgtctc catcagttac ttttttaatt ccatcttcta aatcttcaac actaaatcca
     8581 ctagcatatt ttaattctaa tttcttacca ttactaactt gtttattttc tttgtcattt
     8641 ttactacatc ctataagacc tcctaccata acaattgcca gaataattaa aaagaactgc
     8701 tttaaaacat ttgatttaag tttcatctct ttcactccaa tatcttattt tttatgagtg
     8761 gctaaaaaac tataataaga tatgcttttt aaagtatatc ttattatagt tttcctaaag
     8821 tcatctttaa taagtataac cttttaataa aataaaaaac ttctcctagt ttaataagga
     8881 gaagttcata tatcaacttt acaaaaaatg aattgatact tcattttatt ataaattaca
     8941 atctccaccc taaattgaac ttatataata ctgggcaggt ctcctgactc acggcttttc
     9001 ctccatcacc cttcccaaaa tttttataag tttcagtgga ttttgatttc gtatccgtat
     9061 acagtagcgg gggctgtagt aaattttcat cactttccct attaaactaa tatagtaccc
     9121 tagtattatt ttaatttaat ttttattaag ataattgtaa tttattgttg acctctttgc
     9181 aaggtcgatt taacatatat ttaattcatt catcaatttt ttaatttcac atacttgttt
     9241 ttcatttggt tttacaaacg gagcagtcat ataatcgctt atttgtagcc ctctaatatt
     9301 catggctttt ttcattgctg gtataaatgg attggttatt aagtaaaaat ccattgcttt
     9361 gttaatatat ttttgaattt ctataacctt atccatatct ctattattgt aagcttcagc
     9421 ccatttggaa caaacttctg gaattagatt tgatagtccc cctatacaac cagctcctcc
     9481 agacattaga ttatgtataa agttttcatc ataaccacta taaaccttaa attctgggaa
     9541 ctctgctttc atagtattta ttaaatctct ggtatgtccc atcaaagtta ctgtatcctt
     9601 atatccaact atgtttttat gttttcttgc aagtcttaat gtaagttctg ggcttaaatc
     9661 ataaccagtt ctatctggat aattgtataa atatatttca gcttctgtat tcttagctat
     9721 ttcgctataa aataactcaa tgctatcatc atcaagagag aaataataag ggcttattat
     9781 cataactcca tctgctccac tattatgtgc atagtttgaa agttctatag attcatttat
     9841 acacatacga cttgtaccta taaatacttt tactctttta tctacatgac tagttactaa
     9901 atctataagt tctttttgca tatccataga catattaaag aactctccag tacttcccat
     9961 tatgactagt ccatctacac ctccatctat aagatggtcg tacacttttt tattaccttc
    10021 tctatctaaa tttccttctt catcaaatat tgtaacaact ggtgtcaaaa attctgcttt
    10081 cataatttaa actcctaaaa tttattttaa tatgttcatt aatacatcag atttagcttt
    10141 aatattaatt atattaaaac atttacttaa tgtaattttt taaaattcca atactctcaa
    10201 tctcacattc aaccacatct ccactactca tatatcgagg aggtgaaaat cccatcccta
    10261 ctccagaagg tgttccagta gctataatgt ctccagactc taaagtcata ccttttgaaa
    10321 gttctgaaac tatatctgaa acgtctgata tgaataatct tgtattagaa ctttgtcttt
    10381 cctcaccatt taccctacta catactttaa gttttatagg aaaaggaact gaacttttat
    10441 gtaaaataca tggacctaaa gaggtaaatg tatctaatcc ttttcctcta aaccattgaa
    10501 catgttcttt ttgcaacttt ctagcagaaa tatcatttat aacagtgtaa ccaaagatat
    10561 aatcttctac ctcttctttt ctaatattaa cacctgtttt acctattact actccaagtt
    10621 caacttcata gtctagttga ttatttatct caaaatgacc atctatctca tcttctggac
    10681 ctattgcctt tatcacacgt tttgtaaaat aaactgtttt ttgtggatct tcaaaactat
    10741 tatcaaagtg agtttgtgtt tcttcaagat ggtctttata gttcactccc acacataaaa
    10801 tatcatgtat tggtttttct ataggaacat gtatagtaca tctctctctt ttaaaaacat
    10861 cataattata gaatgtactt tcatttataa tagttttcaa cttaataata tcttcactag
    10921 atatattttg aattaattca tttaaatctt taaagtttct actaagattt aaagaattaa
    10981 catctataat aaataattca tctttagaaa aaactccagt tcttttacat tcattttccc
    11041 cttcagtaaa actgacaaac ttcataatct caccctttat caaaattcta tgtatttagt
    11101 ttcttactta aagattatac atagttacat actctagcta ttgccatttc agtatatcca
    11161 agagattctg cttttgattg ttttccattt gctacatcta caaccatatt tagcaaatca
    11221 tctgttagtt cttccattgt ttgtggaccg tatataactg gactagcatc aaaatccata
    11281 ttatcttcca tatttgcaaa ggttatttta tttccagtta tttttattac tggagctatt
    11341 ggatttcctg atggtgttcc tcttcctgta ctaaacacca ctatttgtgc tcctcctgct
    11401 accataccag caacagatga agcatcattt ccaggtgtat ccattataac aagacctttc
    11461 ttattaattt gtttagcata atcatatact tcacttattt gtctatgacc acctttatgt
    11521 atacagccaa gtgatttttc ttccaaactt gttaaaccac cagctatatt tccaggagat
    11581 ggattacctt ctctaacttc ttctcctacc aattttaatg aattttcata tctatgtact
    11641 atatgtaata ttttttcttt tacttcctca tttactgctc ttctagctaa tatatgctct
    11701 gcacctataa attcagtagt ttcacttaaa attgatgttg ctcctaaatc aacaagttta
    11761 tcacttaatt cacctatcaa cggatttgct gctaatccac ttgttgggtc agacccaccg
    11821 cattcagttc ctagtataag ttcagatatt ggaaattctt cttttctaag caaagaagct
    11881 tcttgtgcca tttctctagc ataacgaaca gccttttcta tagtttttaa agttccatgt
    11941 tcttcttgta ttacaagagt ttttattggc ttatttgttc tctcttttat tgcatcaact
    12001 accaaatcat tttggcaatt ttcgcatccc aaagatacaa ctattacacc atatacattt
    12061 ggatttgctg cataaccagc cataacatcc atagttaatt gttggtctga atgtacttgt
    12121 gaacatccat tctgattatt aaaggtaact gccccttgaa cttgtgatgc tattattcta
    12181 gttgtatctg atgcacacac actagtaggc aatattaata tgtggtttct tataccaact
    12241 cttccatctg gtcttttata tccataaaat ttcatactga tttcctccta aaatagtcta
    12301 tatacttttt ataattttca tatattataa tttttattac actcaataat caaacaatta
    12361 aaatcctatt taacaaccat tcaacaccaa aagcaatgaa tacaactttt ataaatccat
    12421 aaatattatt tatatattag tcataacttg tttatccaag tcctctctaa cacttctaac
    12481 attatgaata tgcacatgtt gaccactttt tatttcctga tttgcttctc ctatatgttc
    12541 accatattta gtaactttat cacctttggc tatatctttt atagctaatt tatgataaat
    12601 agttatatca tccagtgctg taatcgtttt tattgttttg tcttttaatt taaaactaat
    12661 ttcactacct ttagctattg cttcaatagc tactgcaaca ttatcttttt catcaattat
    12721 aactgcattt atcattattc ttgctctcct ttataaataa tataatttta ttaatatcct
    12781 aaaacctaat taaagaattt actattttac caacaactca ctgtaatata aattaagctg
    12841 ttattgtacc atcctcattt tgttttggga acttaggaca tccatacttc ttagcccacc
    12901 aactagttat aagaggtacc aatattgcag tgacaactac tgcggctgcc acctgtgttg
    12961 tagctgttgc aacatatggt tgccatgatg ggtcaactaa tgcaacagca gcaggtacag
    13021 caattgcatt tccagcagtt gtagctacag cccatcctgc atatcctgga cgttttccta
    13081 taaatttatc acaaaatact ataaatatac ctcctacaaa tacagatatt aatccaagta
    13141 gtactcctga taaacctcct tttaatatac ttgcaagatt tatgctacta ccaagtgcaa
    13201 atcctacaaa aggtacgagt aaattacacc ctggttctaa aaactctttc atttttttgt
    13261 caaaatttcc tattatcata ccaacgagta ttggcactat tgcagccaat aatgacaaca
    13321 aaggtacatt tgcaagacct gaagtcccaa gagctatcag tgtaaaaaat ggaccatcat
    13381 ttaatgtaag taaactcata gttgcacaat ctgtttcatc tccatatgaa gtcatcaatg
    13441 aaagatatat actaccatta ctatttgtaa ctgcgcttat aactgctaaa gttgtaagac
    13501 caagtactcc atccattcca aataactttc ctataagtat acctatcaaa gcacctatag
    13561 caaatttaga accaagaagt ataccacccc tttttaaaac ttttggcatt tctctaaatt
    13621 gaagctgagt tcccaagcat accagttgaa ctccaattat ggttgcagac ccaacacttg
    13681 aaaatactgc tgtcgtaaac gaacctattt gtacaatatc tgggcaaaag gtattaatga
    13741 aagccgctat aaacattggc actatcatca tacctgcagg aattttttta ataaaatcca
    13801 ttatcataaa gttaatcctc ctaaaacatg gtatttaatt actcttatta agtttttagc
    13861 tttaattcta acggccgttt tagttcgtat ttaagaacta cgtttttaat tacgaatctt
    13921 tgaattaatg atagcatttt cctatttatg agtcaataaa aaaaataaat ttttgtcaaa
    13981 attttttcaa cttttctgat aagacattat gtattatcaa atgacttcct tatatgcaaa
    14041 aatacatagt atttaaaatt atatggtatt caaaactata aaatttttat acaatctaaa
    14101 aaatagataa attataaaat ataaattcat aatattttga tatttatata taaaaataac
    14161 tattacatat atttacttaa attatataac taaatatcac ttgctttctt taggtatatt
    14221 ttggaaatta gttactcctt cctatacaac ctaaaaaaat atctatattc atctaaatta
    14281 agagcttatt tatatataaa tttacatatt tattttctac attgtaaaat atcgtatagt
    14341 gtgctaaaat caacatattc taatacttta aactaataaa aagagggggg aaatttatga
    14401 cttcagataa tcatcgccca acagctagga tattagatat tttagaagca ttagcagaat
    14461 ccgagaatgg atatactctt acagaaatag ccgatgctat caatgctcca aaaagtagca
    14521 tctttcctat agttcataca cttaatgcta gaaaatatat tactattgat aaaaacacat
    14581 ccaaatattc tatagggata cgttcttaca cattagggtc ttcatttttt gaaaaaagaa
    14641 ctatttttaa ctatataatt gatgaaatga ctaatatagt taattcctgt tctgaaactt
    14701 gtcaacttgg tatacttgac aataatgaag ttctttatat tggaaaagta gattctccag
    14761 aaccaattag acttatttct tatgttggga aaaaaatacc tgctaattgt acaggtcttg
    14821 gaaaggctct tttatgtgat tttgataaag aaaaactaat aagcttatat ccagaaggtc
    14881 taaaaggctt tacagaaaaa tcaattacag atttcgatgt tttatatgac cagcttcttg
    14941 aaattcaaag aacacaaata gcttcagagt gcgaggaatc aatggaacac ctaaaatgtc
    15001 ttgccgtacc aataagaaaa aatggatgta taagagctgc tgttagtgta acactgccct
    15061 tatttagagc taatgacgaa aaaatagaac taataaaaaa attactctta aatgctcaga
    15121 aaaatattca atccatgatg gaagaacgta atgaagatat tgtaataaat tagattaata
    15181 ttctgtatac ggaagatatt aaaatataat aattaaaaat tttattttaa actaatgtat
    15241 ttatttgatt tatactataa aaacaaagtt gctgatttaa ctacagtaac tttgtttttt
    15301 tataatattt tttatatttt cattgacacg ataaaaatac tatgttaaat tctaatcata
    15361 atcgtaatta cgaatctagt tcttatataa gaaccaccaa acctatatca atctaacata
    15421 cgatatatta tgggtaaatt agtgaaaatt gttagatata tatttttaca taaaaatcaa
    15481 tgaaaataaa aggagatttt agttatgagc atttattatg taccaccaat aaatttactt
    15541 gggaaaggat gtcttaatga agctaaaaat tcaattaaat cattaggaac taaaaaggca
    15601 tttgttgtta gtgataaatt tctagtttca aatggtaccg taaaaaaagt aacagatatt
    15661 ttatctcaaa tagatgtgga ttttgttgtt tataatgaag ttaaacaaaa tccaactgta
    15721 actaacgtga ataaaggact tgaaatatta aaatcagaaa attgcgactt cgttattact
    15781 ataggaggag gttctccaca agactgtggt aaagctatct ctatattagc tactaatgga
    15841 ggagatatta gagactatga aggtatcaac aaaacttcta aaaaatgtct tcctatcgta
    15901 gccataacta ctactgctgg tacatctgct gaagttacaa taaactacgt tataactgat
    15961 gaagatagac atgtaaagat gataatggtt gataacaact cactatctac aatcacagta
    16021 aatgacccag aattaatgat tagtaaacca tcaaatttaa cagcagcaac tggtatggat
    16081 gcactaactc atgctataga agctgtagta gctaatggtg cttatgatgt tacagattca
    16141 actgccttat atgctataaa gcagatattt aaatatcttc caagagctgt taaaaatggt
    16201 aatgacatag aagctagaga acaaatgtgt tatgcttgtt ttttaaatgg aattgccttt
    16261 agtaatgcag gtcttggaaa cgtacatgct atggctcatc aattaggagg tttatatgat
    16321 ttacctcatg gagtatgtaa tgccatgcta cttccaatag ttgaggagga aaatgctaaa
    16381 tcatccccta gcaaattccg cccaatagct gaagttattg gcatggatgt aaataataaa
    16441 tcagataaag agtgtgttga ctttgtaata gataagataa aagctctttc tgaagaggtt
    16501 ggaatcccaa aatctttaaa agatgtaggt gttgataatc ctaattttga attgcttgca
    16561 gaaaacgcta tgaaagacgc ttgtgctggt gctaatcctg ttttctttga taaaaagaaa
    16621 attatagaat tgtttatgaa aatatcttaa ttataacaat ctatcccata aattaattct
    16681 gtatatttat aagagagtac ctcaagatgt ctttattttg ttaatggcac tctcttatta
    16741 cactttttaa atagtactct ttatgagaag ttaatatata gccttactta tttaaaaata
    16801 attatctcaa taaaaaactt aaattttttc ttaatggatt ataacagttg ttgtatctgg
    16861 tatattatca tatatccact ttgcattttc cgtacttaat cttatacatc catgactaag
    16921 agcttcacca agtctgccat ctattatata acttcctgtt gaatcatata gtacagaatg
    16981 atagtaatat ccacctttaa ttctagtagc atacttaact gaatactcat ccgttccgaa
    17041 agaaggtttt cttccactta tataaaatat acctgttata gttggagtgt caggcttacc
    17101 tatagtacat tgccacttat aaagttgtcc ccatcctcca ttatctttct taaaaatata
    17161 agtatattta tttctaaggt ctgttacaat tagataatcg ctagtacttt taatattatt
    17221 ttcgttaaca aatttaatca tatcagtata aaaatactta taatctaatt catttggttt
    17281 atttccatca gttgataaaa aataattaaa aacatatcct gttttattat catatacaac
    17341 cttactccaa tctccatcta cttgaaggac ttcaacccta gatttttcag gaattactgt
    17401 aattatattg gaagttgtag atttatcttc tcttaagttt aagttactcc aagcatattc
    17461 gcttactgat accaaatcct tatacacata tccttcttga gaattataca tcaccttaat
    17521 ccagtcatct tcttcatcca atacttccat tttagctccc tgaggtatta ctgttatgat
    17581 acttgaatta gtagatttag cagacctcaa atttacattt gctaaagcat atttatatat
    17641 aggtgctcta tattttttaa tatttagttt agttaatttc acacatatcc atcctctcat
    17701 atgtcttttt acaacatttt atgaaattcg atatgtattc gttctcctat tttcaaactt
    17761 tacttgtatt actaataata ttgctatata tacttatatt ctcatttcta tttatctact
    17821 tatttaaatt tacgatattg cagtctttaa tatactcttc aattatacct ctagccacat
    17881 taaaataact tatacactta tcaactataa ctgcatcact actaactact ctatctaatt
    17941 tttccaatac aacatctgca ttttttacta attctacttc agtttcaatc ccccaagttt
    18001 ttgcatgctc taatatccta tactttaaat ttcctgaatt agaaactgga gcatctaaat
    18061 aaaatttaag attctgtgca ttgtatttat taaaaaattt acctatcaga ttcaaagctt
    18121 cttctgtttt atttataatc ttataggttc ccctaagacc tgctaaatct cttatattgc
    18181 catcacttcc tattactaga gtaccaccag ataatgctac ctctatggat attattaaat
    18241 taaagccatc tatatttacg gtattttctt tgattttatc taaagaaagc ctctttgatt
    18301 gtcttataat atttttttca tcagtacaga ctgccctttt taatgcatct ctttgtctta
    18361 tagaaaactg gtatctatca ccaacaaaat taacgattgg gtctattttg taatctctat
    18421 ttaataacca ttctatttct tctttagcct ttataagtct tattaattct ttacttgaaa
    18481 accatctttt atcactatca tcaaaccctc ttttagaaat cttatttata tcatttcacc
    18541 accttacttt aaatatctaa ctatgctata ttaaatacag attatttcac cttttaggat
    18601 aatttttaat accattatct tatccattta ggttattcaa tatttataat taattataac
    18661 gcttaactcc acttaataat acaaaataat agattctata ttatatttat aactttataa
    18721 cataaataat tagtgtttca aaataaattt ttagttcata acgtaatctt cttttataaa
    18781 aacaaatata tttttatcat ttaaacagta aaaaagagac ttatctctat tttgagatag
    18841 tctctttcta aaatctattt ataatgataa ttttaagctt tatatattaa acttatatta
    18901 tacttctttt atcatactag gagatagaat atcattaaga gcttctgtca tagttggatg
    18961 tgcataaaca cggtctctta agtatgtata ctctacttct aaatccacag ctaattgaat
    19021 caaatggata atctcacttg aattttcaca tatcatacta gctccaagta tcttattgga
    19081 ttttttatct atcactattt ttataaaccc atccgtcttg ccaatctgtt tagctcttgg
    19141 aattgcttca attggcatat ttgctactaa tactttatat cctttttcct tagcttgttt
    19201 aacattaaga ccaactcttg aaaatgctgg acttataaat atagaatttg gtatattctt
    19261 tctatcacta gttgttctag tcttatctcc aaataactga ttaataatta tgcgataatc
    19321 atccaatgaa atataagtaa attgtggacc tccatttata tctcctatag cccatatatg
    19381 ctctttatta gtcttaagag tttctgacac tttaataaat cctctttcat ttaattctat
    19441 acctgcattt tctagtccta acccttcagt atttgccttt cttcctgtag ctacaagtat
    19501 tatattactt gctaattctt tagatttacc atctacttca tattccacta tggctaattt
    19561 agaaacttct ttaatctctt ttatatttgc attatttata atttttacat ttctcttttc
    19621 gagtaatttt attatttctt ctgaatcctc aacatcttca tttggtaaaa tgccattatt
    19681 agagtttaat attgtaactt ctgttccaaa tgaactataa atacctgcga actctaaacc
    19741 tataaatcca gctcctataa ttgtcatttt tttaggtaaa gttcttagat tcataagaga
    19801 ctcactatca tatactacat tcttattttc aattccctta atattaggta taaatggtct
    19861 tgaaccagta ttaataaata tattatctgc tactaactca taaattttat tttcagtttt
    19921 aacttgaact gttttttcat caatgaaagt tcccattcct gtgaatatag ttacattctc
    19981 atttgaattt aatttattat agttagcttc tctaagtttt gtaattaagg tttctttttt
    20041 atctatagcc ttctcatatt caacttgtac ttcatcccaa gaatttatat tctttgtttt
    20101 tacattattt gctgaatttt ctaagctttt tgttggtata catgctacat ttatacatgt
    20161 tccaccatac atcttgtttg acttttctat taaagctact tttaatcctc tatttgctaa
    20221 atttcctgct aatgtttttc caccttttcc aaatcctatt attatagcat caaatatttt
    20281 tttcatataa atttcaatcc tttcttgtat tgtttatact tttactataa caagattacc
    20341 taagtagtac aattaggcac ttttttgtaa ccttaaactg atttataaac ttctacttaa
    20401 atacttttat ttatttctct ggttttcaat aaaggatgcc atagttgcct tatccatagc
    20461 tcctggaata tactgagtta catatccttc cttgtcaatt ataaatgtag ttggaaaagc
    20521 attaattcca tattgatatg ctaatgctcc atcttcatca agcacaacag ggaaggtata
    20581 accttggtct tttaagaagt taactacatg ttcccttgaa ccttctctac ctaaattagg
    20641 tgaagctact cctaaaatta ctacatcatc attattttta ttatagtctt tatacagctg
    20701 ttctatatga ggcatttctt ctttacatgg aggacaccat gtagcccaga aatttaagaa
    20761 tacaactttg ccttcataat cacttaattt atgtgtttta ccatactggt ctgttaaagt
    20821 aaaatcaata gactttattc tatcttcatc attagaccct tcatcattat tttcattatt
    20881 agagtctttt tgactatcat taccctcaga acgttcttct ttattagtag aattttctct
    20941 cttattctct tcttgcttac tttcaatttt gctattttgt gacatattaa agtgtttgct
    21001 tatacttcca aatccattta ccatcattaa tattcctgat actataagta taatcccacc
    21061 tattttttta ataatttcca tattagattt tatcttatct atagttttaa atagtttact
    21121 gtaaaacata gctgtaatta tgaatggcaa aataaatcca atggtatata ctgctattaa
    21181 aagatttgca ctaagatgat tacttgaact tgaaaccatt actaatacag atgccaatat
    21241 tggaccaata caaggtgtcc atccaaaact aaatgtaaaa cctaacagga aagctgttat
    21301 tgctctcatc tctttaaact taacattaaa tcttttttct ctattcaata ttgaagattt
    21361 aattattccc atgtaaaata atcccataat aataattata atcccaccaa taaacattat
    21421 taaatcttta ttttcattaa aaaacatact caatactttt actgatgaac ccaatataaa
    21481 aaatgttgtc gagattccaa gagcaaaaaa tatagtattt ttaaataaag aacttcctat
    21541 aaaactcgtc tttccttctt tcaaattctc tacactacta tttgacaata tgctcaaata
    21601 aataggcaaa attggcaata tacatggtga aaagaatgat actatccctt ctataaacac
    21661 taaaaataaa tttacatttt gcaaattaaa attcctcctt attttatttc caatagtaca
    21721 atctcatttc taaattaaaa tctgactaaa aaattatgta atttcaatac atttttctac
    21781 ttataattta attaactctt cactacttcc agagtaaatt ttagtattta tcacaattta
    21841 aatttttatt gctccacata atcatttttt ccatgacagg aagtaatagt aatcctgatt
    21901 cacttaaaaa atatgttgat tctacaatat tattaaccaa ctttttttgg ttgactataa
    21961 taccctgttc aattaaaaaa tctagttgtt gaactaacat tttcttacta cagtctggta
    22021 tagctctttg taactctcca aatcgttttt tatctctttg tattttccat ataattatag
    22081 ccacccattt tttacctaaa atatttgata aagcttctac tggacaatta tatatcatat
    22141 catctatgtt gtacatttta ttctcctctt ttccttattt aatttattaa acaaactttt
    22201 atagcaatat atttattaat attataaata tacctagctt tatttaatta aaatttttat
    22261 tatatatact cttattacaa actattttag attttatgta attaatatat cagttttata
    22321 aatacataac aattggtaac ttttttgtaa ctacatcaac tctattgata tacaatttct
    22381 aactcatttt attaaaattt tattgatttt aaattatata taaaatcact ctaatataat
    22441 aattctttat ttaaattgtt tatcctgtta aactaattat tctacttcta taaattctat
    22501 attaaataat aaatccatac attaacattt acccaaattt atccaaatta aaaagtctgg
    22561 cagattaata tctgtcagac tttaaacaat aaacttacgt ttacacttat aaatccaata
    22621 atattgaaat aacatcaaaa ggtgctattt catataattg ttcttcttct acatcttcta
    22681 ttgcatttac aattttccca tttatgtttt taaatatttg ctcatctaat atcattctct
    22741 tactactttg gttttccaat cttattacaa atttatcttt actataatag gaaggataac
    22801 atgctgatat aagatagttt tctggtaaag atattatttc taattctcta cttacagttt
    22861 tattgatgat tgatttttga atcttattgt ctatacgata taaaaagaaa ttataatcct
    22921 gtaattgata gtttacacat tcccttagat aattgtgggt aaaattcgca atttccaatt
    22981 catcaaatga tttagaatcc atatatattg caaatgaaaa ttctaatttc ttaaatattt
    23041 gagctttatc tgttttaatt cttatatgac cttttttagt tgtatcacca gaagcacgtc
    23101 ctggtctata taataaatca ggctttccta gttcatctgt agtagcaagc aaggtcaatg
    23161 caatttcatc tttaatatgt tgatactctt ttattcctct agtaaataca gtacaacttc
    23221 tattttggtt tgtcagcgta atattacttt ccaaaggttc taaatctata ggcatctcac
    23281 tgtaaatatc ttcccagttg tctaaaactc cattttctct tgttatatat ccaaatggca
    23341 atgatgctat atttttatta tcttttatat cagtttttat atgaacacgc aatctatgat
    23401 tatatatcgt gttattaata caaagtttta catccatcaa tggattttct ttggataata
    23461 taataattac ttttacattt aatttaccat taagatattt gttttttcta tcttctaatg
    23521 taagcggtag taaagtagta cctttaacta ccaacttttc atatcctaaa gctttttctg
    23581 tatacgctct atcaaatgaa agtttgatat cctcatcatt ttttaatgga gaaaaatcat
    23641 aggtgtcacc tgcattacca ctatctttta atgttaaaaa gtttgatatg taagttccat
    23701 ctttaagttg taaatttaaa cttccatctt catagattac cttataacta tcattagata
    23761 taaaagaatc attagattta ttaagtgata ccatttcttt atcacttgat tcaaagctaa
    23821 caactttata accaagtgca ggaagctgta tatttattct tacttttaat atataatatc
    23881 ctggttcttc aataaataca ttgccagcag gtgtttcttc taatacattt tctcttggtt
    23941 ttacataagt ttcttcaatt ataatagcat ctaaattatc tttaaaataa atatttttac
    24001 tatcacttac aacttgtatc tctttatatc cattaaagcg cttggcttct gtattaaata
    24061 ctaaaatgtc atttttagat aatttaagcc tttctgaaat ttttttgaca atagtatttt
    24121 caatactgtc acatatttca tttgcttctc ttatacggtg aagaatatca tctgttacag
    24181 tatcagttac acatccagct aaactatcgt gtgcttgccc ttccaaaagt tttttccatg
    24241 tatttaaaag taactgattg cttatttcaa ttttactctt tttagcaatt actagtaatg
    24301 gttctattct tttaatcagc ttgttttcta gtttatcaca tgctaatttt atatccattc
    24361 tactagaacc aattgattta tgcattcttg ccaatacagg ctctctaaat tcacctctat
    24421 agctttctag cgattctatg gattttatat attttaaaaa ctcttgatat gaactcgtta
    24481 tatatgaata ctttccgata tcattaatcg ctttaacttt atttttgaaa tcacctatta
    24541 tatttagctg gtcatttcca gaaggtatta acacctcatc tacatcagta tatgatttaa
    24601 taaaatcaat agctgggtct aagcgtccat taacatactt tgatgaaggt tctaaaagca
    24661 taccagttcc atatccatgt ggcatattta ctgcatatat ataactttct ccatttaagc
    24721 ttttccactt aaaatatggt gattgaacct gtttaccaag atgtatacct ctccaaaaga
    24781 atatattatc aagacctaca tgctttaata gtataggcat ctgtgcatta aatccaaatg
    24841 tatctggcaa atatccaatt ttcatatact taccatactt tttactttca aaaattccaa
    24901 tcattaagtt tcttaaaata gactctcctc tagcaaagaa tgcatcagtt tgagtaaacc
    24961 atggtccaat atgtagtcta tcttctttta tcaacttttt aatgatttct attttttctt
    25021 tgtataattg tacataatca tccaaaatag aaagttgtcc atcaagtaca aaacttacat
    25081 ctgggttacg ttctaattca tcaattacat ctgtaaatag ttgttcacta agtaccaatg
    25141 catctccaga agtaaaatac cattctctat cccagtgtgt atgattaaca atatgtgctt
    25201 ttatcattta ttctcctctg ttctttatct ttaattttaa gcttcccact ctgattcatc
    25261 aaaatcatca tcttcaaatt catcatcttc aatcactgga tttccacgca atactataac
    25321 taacccagct acaattactg ttccaactac tattgataac acatacattg gccaattctc
    25381 tactgtaaac catccataaa atccactaat tggagttgca tttactgaac ctaaaccaac
    25441 tgccatagct gaagcaattg cagttccaat tacgcatgaa ggtattactt ttaatggaga
    25501 ttctaccgca aaaggaattg caccttcact tactccaatt aaccccatta taagagatga
    25561 cttcccagct tcacgtaact gtttattata tttttgtttt gctagtatag tagcaagtcc
    25621 caatcctagt ggtggtataa ctattcctac cattgcagca gtattaggtg cataaattcc
    25681 acttgttaat aatgcaagtg atgttgtcaa agctgcttta ttaactggtc cacctaggtc
    25741 aactgccatc atagccccta taatagccgc cattaatatc tgatttgtac cagataatcc
    25801 atttaaccaa ttttctaatc cagtatttaa tgtggcaaaa ggtataccta tcacatagtt
    25861 aattaataca acagtgatta atgttccaaa tactggtact ataaatattg gtactattga
    25921 agctgctgat tttggtaatt tgacatgatt tttaataaat aaacatgtat atcctgctat
    25981 aagaccaact gctaaagctc ctaaaaatcc tgcacctata tctttagcaa tcataccccc
    26041 aacaagacct ggtacaatag ctaatttatc agctatagaa aatcctataa atgcagcaat
    26101 tacaggaaac attaatccta atgcagtacc tccaaatcca tctaaatcat gcaataatct
    26161 aacaatacta ctagcacttt ctgcatattt agcatcccat atatctggta tattatagaa
    26221 agaaccacct acacgagata ttgccattat catagcacct gcaattacta gtggaatcat
    26281 atatgagatt ccagttaaga catgcttttt taactcagag actatctttt tcaacattac
    26341 atttccctcc atttatttag tgtcaattaa ctttaatgca tcatttatta ctttctctgc
    26401 atcctttata ggcgcattaa ctggtacttc tagtatttct tttccctcaa aacgttcagc
    26461 atttctgact ttagtgtcta ctgcaaatat tactacttct gcaatttgtg catcctttgt
    26521 agttagctca ttatcgattc ctgtagctcc ttgtgtttca acttttataa ggtgacccat
    26581 cttttttgaa gctttcttta atgcttgagc tgccatatat gtatgtgcaa ctcctgttgc
    26641 acatgctgta actgctataa tttttctttt catctctaca tctctccttc ttcactaaat
    26701 atattgatta tttcttgttc tgaattggct aattttagtg catctacatt gtcatcatcc
    26761 ataagtttag ttgccaattt agataatagt acaagatgat tgctatcctt atcaatattg
    26821 cttatagcca ataagaaaat gctatctact ggtttttcat ctaaagcatc ccactcaatt
    26881 ctatgcttag tctttgcaaa tattatagaa gatttcttga cagcattact ttttccatga
    26941 ggaatagcaa taccattacc aatacctgta gttgaatgct cctctctttc caaaacagac
    27001 ttaacatatt cttcttcatt gtccacaatc ccattttcaa caaatatact acttaatttt
    27061 tttattgttt cttcctttgt atttgtatca agattaaaaa caatttgttc ttttttcaat
    27121 acatctgata ttttcactat atactctcct ccactagcaa atcgtacact tcctttgtag
    27181 aagttgcact taatatgtca tttatatttt ttttattgtc aatgaagtca taaaaaactt
    27241 catatatttc atcaagctct aggtttttgt cattttctaa cgaaataaag aaaataactt
    27301 ctaccttttc atttccccat gtaataggat tttttaacgt tgcaacagat atattagatt
    27361 ttttaatata actaggatta gcatgaggtg tagctattga ttggaaagat gtgtaggata
    27421 aattttctct ctcaattgca cttttagcta taccttcttt tgcaaaacat ttttttatta
    27481 ggttatctgt tatatactta attgcatctt cataatttga ggcttcaata tttaacaata
    27541 tattttcttc aaagattaaa tttctgaaag atttatactt cttagaagta caacctgaaa
    27601 cattttgtat atttctttcc aaggttctta tatcactttc atttaaaata gggctaacta
    27661 ctatcgttgg aatagtttct agctctaaat aaatagtact tattactaag tctgcatcaa
    27721 ttttactaga tactaattct tgtacagaaa gcactttttc aatctttatc attggaaagt
    27781 atttttttat tcttgcagac aataacctag atgaacctag ccctgtacta catactagaa
    27841 ttactctaat attttgtgga agttctttca ttctttctct aaaagcttca aaatgtaatg
    27901 ctatataagc acattcatta tcatttatcc taatatcata tataaattca attactttct
    27961 ttaaatctaa agcttgctca aatgataaag aaaagttttg tttgattgta tttacatatg
    28021 gatttttaat actaagtcca tatttcaatc tatttattga tgaatgtaca tgtgaagtca
    28081 atccatcaat taaatcctta tcataagctg tctcatacaa tgtatttttt ataatttccc
    28141 gaagatttga tacattattt gtatttgttt cacaaggatt agttgaagta ttttttaatt
    28201 cattagtatc tctatgttgg tcagctgcta ctaaatgtaa atttatatat ccaatttcag
    28261 attttggtaa taatattgaa aatttttctt ctaattgttt tgctaaataa ttggtatttg
    28321 acatttgatt attttctata ttaccaatat acaaactttc ttctatatat tcttcttttc
    28381 taattctctc tacagctatt gccaaatgaa taactaacga ctgaaattca taatcagaga
    28441 actcaaattt tctttctctg ctgaaattcc taagaatttc aataattatt gataaatcat
    28501 cttcagaaaa aataccattt acatctgcct gtatcttctc aaatgcttga taaaagcttt
    28561 cttgctcttg cttaatatac cagtttctac tccaaaattt atgtataaac ttagcagtaa
    28621 gttgtctttt atctctttca cttatattta actttattcc tttactaggt ttcttatata
    28681 tggaaatccc ttcatttttt aacaattttt cgacttcggc taaatccctt tcaatagttc
    28741 cacgacttat atataattcg tcagaaagtt gttgtatagt gatatagtca ttactcttca
    28801 aaagtactga atatatatag ataactctct cctctctggt tgtaggaatt tgacttcctt
    28861 tattatctag gtaaggaagt atttttttta aatctccatc aatataaaca cctatcttag
    28921 gttttcttac taaagaaaga cctatctttt taagcactgc ttctacttct ttaagagatt
    28981 ttgaaactgt tttttctgaa acgtctaaat cacttgctat tgcttttaca gttgtatgac
    29041 cttcttttaa taaaaacttt attattttaa tttcacgatt ttgaatcata tcagttaaca
    29101 ttttcaattt tactccttcc ctttgtcttt attctaatgg aaaatgtttt tctaaaaaac
    29161 tactaatatt tcctgattaa atacggtaat ttttatgtat tgaatacttt aattttagat
    29221 gataaattaa aaactgtcta aatttaatat gaaaggaaat agtttaatgc tgacaataat
    29281 tattgcttta cttcctgtaa tgggatgggg attgatgccc atagttgcaa atctaaagaa
    29341 aagttctcct catgaacaac ttttaggtac atccataagt gcgtttatat tttccactat
    29401 aataactcta gttatacatc ctgaaattac atttttttca ttttgtataa gtatggtttc
    29461 tggcatattc tggagtttag gacaattaat gcaatttaaa gcaattcaaa ttgctagtgt
    29521 atctaaagta atgccgattt caaatggttc acaattaact tttacgacat tactagctgt
    29581 aatcttattt aatgaatgga caagttctaa aatgttactg attggttctt tatcaatatt
    29641 atgtataata cttggtatac ttctcacaaa ttatcaaact aaaaaatcta cagataaaaa
    29701 tatgttaaaa tgtgttggag ttgtattatt atcatctcta tttttatcat tatatgttgt
    29761 aactaatcaa atatttttaa ttgaaggata tagaattata ttacctcaat ctgttggtat
    29821 gctgataact tcatctatta ttgtcaaata tttttctaaa gagattgtat ccagggaaaa
    29881 cgtcttattc aatttaatta ctggaatatt atggtcaata gcaaatttag gtatgttcat
    29941 aacgactaat acattgggag ttacaataag cttttctatc tctcagagtt gtgtgatagt
    30001 agccacttta ggagggatta tattttttaa ggaaaataag aacaaaaagg aatggttttc
    30061 tattcttatt ggtataatgc taatcatgtc tggtgtcttt atgttgagta ttataaaata
    30121 ggtatttatt tacacaaatc atatacattt ttatacatat aaattaactg tttctattta
    30181 ttaataaatt tacctatata cttaaaataa tagcatatgt atttatgaaa ttaaactata
    30241 ctctaaatgt aagtatattt cattaaatac atatgctata aatttttctg tcaataagag
    30301 agtacaaaac atcttatatt gtaaataaat ttaactttat aaatttacca actaatattg
    30361 tattatttaa tattaagttt ttcatcttct ataagcttat ctgcaaccca ctctgcaact
    30421 tgacctgtta ttttaataca gtgttctttt ctttcgggag acataaagtt atcaccatcc
    30481 cacttctttg taataactct acaacatgtc gcaccatatt cttttttaat ataatcatgc
    30541 aaccctttag catgttcaaa catctttgga ttcatagctt caccatgaac tcttccatat
    30601 actaaaccta acgccatctc acctccagat attgctccac aaagacattg tgcttttcca
    30661 agtcctactg gaaatcctga tgctaactta gttatctctg gacttaatgg ttgtcctaaa
    30721 gcattattta ttgtgtgaag aactgcttca gagcaaaaaa actctcctct tctaaagcat
    30781 tcttctgcag ttgttctaac ttcttttaga tattcttctt ttgttttcat tttttccctc
    30841 caataaaatt aagctaaatt aagtttctat ggtttgtctt atgttcgtat atttatataa
    30901 atattcctac caaagacaca tatttaataa ttcttattta gctttagatg tagtttcaga
    30961 tttactaaga tgtataataa ataaaactaa aacatataaa ataatcactg tagcaattat
    31021 ctcttctaat ttagaagcat atgaaatcca acttataaaa tgacctgatt tagttaaatg
    31081 accatttatt agtttcatcc ccattgcact tattgccata gggaatgtaa atgcagcaaa
    31141 actcggataa aaaggcaatc ttaataattt tggtaaatat cccaagacta taatataaaa
    31201 cacaattgat aatataaata acaagtataa tactacatta tttttatctg aaaaagaatt
    31261 aatatatcca gacaataata gtgaccctgg tgcagctaat atagctatac ttggtaaaga
    31321 tggttcaggc atttccttga ttatccatac tctcttcaaa attactggta taagaactat
    31381 ataagataca aatcctatcc aaaaagcaat tttgccaatc aaaagttgat taaatgctgg
    31441 agctgtaaca ctagctgcaa ctatcccaac atagactata taccaagatg gaaatactgt
    31501 ttttattgag aatttaatta ggaatttttt actaaatagg actattaaca caagatgacc
    31561 cacaactcct atgctccaaa tcataatacc taattctaaa gataatggtt ttacatatgt
    31621 ggcaaatatc attgttgcca ttgttatagt tggaaatgta cttgatatta agggattttc
    31681 taattgctct ttaacaccct ttctaagtaa tactattttg attatataaa ttgcatataa
    31741 aataaatcct ataactccaa ttacattacg taatgattca ctataagatt gtaataagtt
    31801 acctaaagta aatattgaca aaatcaaact tgcaataggt ataggatatt tttttaatag
    31861 gttcaaatta tgcctccttt gttactaaat tatttatgtt ttaattatat caaaaagatg
    31921 gtcaaattga catattatga ccatctttct tatatagaaa atatctatca aatttaattt
    31981 tattattata attatctata ttcttaatat attaagttaa ttagtatatt tattttaatc
    32041 aaatatacta atttgtttat tcttatctac tttaatagtc ttcatatctc taatgcttat
    32101 ttttgcatca cctcttaaag tatctatgag taatttatta tcttctttat aaagcctaca
    32161 gttttcttct acttttgcat aattaaagtt agcataacaa tacagacaat aatgtctgca
    32221 agtattgtat tgccctatat cgacactttt tacacatcca caaatatctc tttgtgtact
    32281 atcttttttt acctctactt cacatcctat aatttttgat attaatctat catcaataca
    32341 cttacttttt ccaatcccaa atttacttaa attatattct tcagagcaca tttcaagtgt
    32401 tatcccatgc ttatgagcaa tcttagaaat ctcttttgat aatgtttcta tttctgtaat
    32461 ttctattggc tttatatgta atgctttagt attaaatttt gttttcttat ataaatcgat
    32521 aaaacttatt atacattttt ctgtaaatcc atcaagctga ttacataaac tcgcaaatgc
    32581 tttgatatga taatctactg tgtacttttc acttaaaaaa atcgggtcat accttaaaat
    32641 tactctttct ttgcctattt ttctagccaa ttccttaaat gaatctatta cctttctttt
    32701 atctaaaatg caggtctcta cttctttgcc atatgatgtt atagtatagt ggaaataata
    32761 tttatagtct tttaatttat ttaaatcaca tatcattgga gtcacatcct ttgtccaaaa
    32821 tacaaaacaa tcaacagtat gtggatttaa ctctatctta ctaacttgct ttggattcat
    32881 tggattaatt acataaacaa aaccttcttt aagtctgtta aaaaaccact tactatagaa
    32941 agcaggtata tcagttcttc tactcacact tactatcatt tttaacctcc atagtaatat
    33001 aattatgctc taatatattt ttaatataat tatagctcta aatacttaat ttaatcaaaa
    33061 catttaagat aattccagta tgatttactg attcatttct agcatttaaa atatccttaa
    33121 taagtttaca attctttaaa aataaatata tattattcct aaacttctaa aactaatttt
    33181 ataatgaaac taaaaaaggt gctattttca agcaccctaa tccaatctgg agttttagtt
    33241 tcaatttcca tgattaattc ctccttttta aatctaaatt cttctcacag aaacaatttt
    33301 gaactataac tgaagtgcct tttactatct ttgggaattt tgagaatagc actttcattt
    33361 tcacaatatt attataattt tattatattt ttttataatc tcattatatt ttcactatta
    33421 ctaaattact cttaattttt taatataaat tttgtacaat gattaaaata gttaagcttt
    33481 atatcaatat aatctaatcc caataaaaaa aacactcatt tctgagtgtt ctatacaagt
    33541 ttttattttg agacatctta tataatctta tattttaaaa agtttataat atccaattta
    33601 atatcttctt tagtaaagtt atttttgatt tgcatatctt agtgaccatg gacaactcat
    33661 catgcacctc aatacacaaa aacactttga aacatcaata atagactccc tttctcctgg
    33721 caaggtccac tcgtttccat gtatagcttt tgcaggacag ttttctacac aaatattgca
    33781 atctagacat tttgaatcaa tcaaaggatg tctttcaaca gaaattggag cgtctgtaag
    33841 tactgtacac atactaaaag cacatccata ttcctctgtt acaaacaaat tatttttccc
    33901 cataaatcca ataccactaa tatttgctat tgtcttatgt ggcaaaggac taatccctga
    33961 ttgcatttct ggattaatat aagctttttc aaaatatcca tgttctagat tatttttctc
    34021 tgattgagaa taagctctat atcctttact ttgaatatat tttgaaatcc aatcagctaa
    34081 cccttctact ttctcctctt tttctaagaa ctcatcacta cctgtaggta agttattgta
    34141 tatatctgta ataaattttt ttgataaccc tatacaaaat aaaattgctt tagagaaacc
    34201 ctgtgtttga cttgttggaa attcagatat atcaacaaaa cgaacaatat ctacaccttt
    34261 actgtataaa atagtcttta attcatcatt catgtttgac tccttctata ggtaaaaaca
    34321 accttatata ttaataaaat taactattct aattatgtta accaatactt tgtacattct
    34381 ttatttctac tcataaaacc atactcaatt aaatatcttc taattgttgc aaaatcacca
    34441 tatatagatt ctaaaatctg attaacttcc ttttccgaat attgcttccc acgttcaaat
    34501 tgttccaaaa tcttagatag aattacgacc tttttctttt cttttgaaga aaatactttt
    34561 aattttaaag gattcatact ttcaaatgat gtatctaaaa tgtgttttct ctcttcttca
    34621 gttactacat atctttcatc taccattcga gcatgttcat gaattgatat tatggtgcta
    34681 cctttattag atttatcttc aaacacagat tcatatagtg ccaaatataa cttagcttgt
    34741 tttgcttttt ctctgaacat aaatttttga tgacgtatag tagatgtaga cactccaacc
    34801 ttttttgcga tttccttatc aggtaaatca ctaataaaca attgaaataa ttgtttttga
    34861 ttctcagtta atgtgttgta ctttgaatca ctattaatca tttgctctaa gtaatcacta
    34921 tgttctaatt ccatatgaag ttcaatagct tttgaagctt caaagaatct attacctatt
    34981 ttatatattt ctcctatttc atatattttt gtacatttaa tacaactata acaattcaaa
    35041 tcatcattaa atatataacc tcgtttaata tcattaatag aaagattttc aattttcata
    35101 ttactgtctc catttcatat tttttcattt ttataaatat atcatatcaa agtttattaa
    35161 tataatcaac atttttaatt tttgtttata aaatataatt atttattaat ttaaaatcac
    35221 aatatatttc ccacctttaa tatcattacc tacattatta tgtattcata ttgtgatttg
    35281 aatgattagt tattgtaact ggagtaataa gtgatatccc ctttaattca atcttagagt
    35341 ggtcaatttt caaaattgga gttccttctt tttaaattta catcagctaa atcaaaggct
    35401 atcattagtt ctaattaata tttaacattt tatgtaaaat ttataaaaaa atgtatataa
    35461 attaagataa gtgactatac taaaaatatg atacggacaa acaataagtt caacctatct
    35521 cccccaaaat aaaatgagct acagtttccc ccaactgtag ctcattttca tgtaatttaa
    35581 tattttaatt tttctagact ttttacaaac atcaaattac cattagttat tccccataat
    35641 tcatcagcat ataaagcaat tctatcaaaa gttgtagtta ttatcacaca tttattatct
    35701 tcagatgcta gtttataaaa tatatctacc aaactctttt cacctttatc atcagtattt
    35761 tctataatat catcacaaat tattaattca ggattattag ataatgctct agcaatacag
    35821 actctcctaa tatcatcttg agacaaatct ttcactttac aatttgcttt ttctttatca
    35881 attcctactt tttttagaat atcatataca atatcctctt tattctttgc tttatttcca
    35941 gcaatcagca tagttagaac aatattatct aacacagtag catcatttaa taaattatga
    36001 tttttaaata tcgaccctat atacttactt ctgtaaatat ttttattaat attttttaaa
    36061 tcttctccct tatatataat acttccacta gtgcaatcat caagacctga tattacagac
    36121 aataaagtac tctttcctgc tctatttttt cctacaatta catacatttt tccaatttca
    36181 aattctgcat taacatcttt taatacaaac ttactgctgc cttcatattt ataattaaca
    36241 ttaattaatt caagtattgc caatctatat cacctaattt ctatcataaa ttatttgtat
    36301 tggcttacac ataattgcat tatatataga tactgtacta gtcaataata tcaatatcat
    36361 tgatataaaa attatcttaa gtataaaatt taaagttgat ttgtctttta aatttttcat
    36421 ctcttgttca gtgttgatat catttgtatt gacatcttta taactacctt tattaaacaa
    36481 aaaatcatta tatgttatat tatacttata ctgatattca aaaacataat ttgatattga
    36541 tttctctgat atacttgcaa ttcctaatcc aagtacagta gatattaaga ttatcatgat
    36601 actttcataa ataaagcttc taattatctt aaacttagac attccaatta gtctcaacac
    36661 acatatatca tattttattt tttttattga tatggcagaa cttatcacca gcaatattga
    36721 acctagcact aatataccaa gagtatacgt aaaaaccaca ccagataagc gttctattgg
    36781 tgccattaat ttatgatata ataactcatc tatagtaact ttatagatat cacttagacc
    36841 tttatttctt aattctttct caaaagcctt tctactatta gaatctctaa gtaaaaaact
    36901 agcacttgta taaatcattt tactttctgg tctaaccctt ttttcaaacg ttactaaagt
    36961 attatatgag gttaatatat tgttgtatat acttacatct tgaacacttt ctaatttatt
    37021 actatccttt gtcttatata cacctactat gtttaaattt aacttcacat tagtgttata
    37081 atcatttaat aaagatattg tatctccaac cttaaaatta ccagccttta gaaaatcttc
    37141 acttactata cattcattat ctttttgaaa tactcttccc ttacttatat ttaaattgta
    37201 atacttaaga cttttatttt tatctgtaaa tcccatgact atgcaatttg tagttatatc
    37261 attatagtca agttttttat cattactagg tgcatatttt ttattatcaa catatagtgc
    37321 aaggtttcct gtaagtgaca ttttctttac gtattttgat tttgaaaagt ttaaataatc
    37381 ttctaaattt aaagggtcat atccaattaa agattctgtt aaatcatttt gaacaacttt
    37441 tttttcatca attttaatac taacctctga ctcaagcttt ttctcatatt tttgagttat
    37501 aatggacata tttaaattta ttattgagga aatagctatt gataagacca ttaataatat
    37561 gataactata ctaaatagac tatgaacttt atttctaatc aagtttttaa ttgcattatt
    37621 taaaatatac actttatatc tcctcttatt aattaaattc tatattaaat atttttataa
    37681 ttcttattaa ctcttacttg tattctttaa acactaaata aaaagttgaa cctttatttt
    37741 cttcactttc aacccatata aacccattac ataaattaca tacatgtttt actaaactaa
    37801 gacctatacc atacccatct ttatttaatt catttattct atatctatca taaaatattt
    37861 tatctatatg atgctgattc attccaattc cattatcttt tactgataca atgacacttc
    37921 ccttttgatt ttttacattt atatatatct ctttaccttc accatatttt attgcattat
    37981 ccaaaagatt agatacacct ctataaatcc atttttctct cccaaagata ctcatatttt
    38041 catcttcatc aaaatcaaat ttcaaattgc tatacacttt agaataatta tcacatactt
    38101 gtgcacatat taaagctata tcaacttcat acatatcctc attatttttt atataactta
    38161 ttgcaagtac atcttctacg ctatttgaaa ctttatcaag aacatttaat aattccttat
    38221 ttccatcaag ttctaaactc gttctaagta ttgatatagc attcttttgt tcatgtgata
    38281 aatatgaatt taacctttta taatcctcaa tatgagaatc gaatttattt tttaaatctt
    38341 tatatactct agacataata gggtctatac tcagtatttc attctcatct tcaatgttat
    38401 ttatattatt tagtatttct attctttctt taaaatccaa tcgatttaat attaataaaa
    38461 gtataaagtt agaggttact ataaatgcaa aaaatagtat tataacaaca ttaaaattat
    38521 tcttaagtat aagattataa ttagtcatta atcctttatc taaaacataa gaattgttta
    38581 gaggtatttc actcttagat aaagttgtat ctctagaaat taagttctta tctatatctg
    38641 atatatttat aatatatttt aaacctactg tgaaaattat aaaaactata acagaatata
    38701 aaatcaacgc tatagttttt ttgtctttta atccttgcat atagaatatc ctatccctct
    38761 agtagttatt attatctctt tacctgcaac ttctcttaat ttctttttta atcgagctat
    38821 atgtaccctt aatacagatg ataaagggtc aaaatattca tcatagacgt gttctgatat
    38881 atctatacct gacacaatat caggatatct gcttgcaagg tattctacta tatcaaactc
    38941 ttttgtccct aaagttactt cttcattata tacatatact tttctagagg atggatttat
    39001 tgtaattgga cctgattcta tattggggtt tgttctacca taaaatcttc ttataaccgc
    39061 ttgtattcta gctcttaatt ctataatttg aaatggtttt actatataat catcagctcc
    39121 taaatcaagt ccttttgcaa gttcgtttac tccatctctt gctgttacaa ttattacagg
    39181 tgtatttaga ttattacttc taaaaaattc taatatctct attccatctt tatctggcaa
    39241 gtttaaatct aataatatag catcatagtc atttacaaat gctttttctt ctccatctaa
    39301 tccacagtta gctatatcta ctacaaaccc tgcattttcc aaacctgact ttatattata
    39361 agaaagttcc ttattatcct ctattattaa tactctcata taatcatctc ctaaataaaa
    39421 tatcagttta tttattatat ataatgaatg tttattgaat gttaacttta ttttataagt
    39481 atttatattt taaacaaaac acaattttat tattattcaa atgcttgtat ttgtcaaatt
    39541 taagatgaat ttatctaatc atttagttat tatcagcaat ttaaaaaggt gaattcatct
    39601 aatagaaccc acctttttca attaaatctt tattttgaaa tagcatttct tagtattgag
    39661 tgtatgtatt aaaaaaacaa tccctgtaat ctttattaat tttaaatata tctttcagta
    39721 ttgaatccac aatcaataac aacttataat gatttattgt ggatataaaa acttcatagt
    39781 tttatctagt gagcgtcaac aagcataaac tcctttattt tctgcttatc tagataagaa
    39841 attgcactgg atacagctgt ttgaccttgt cctgctgatt ttaaatattg atatggttta
    39901 ccagcacaat ctcctgccac atataaacct tcaatatttg tgttcatatt tttatctgac
    39961 tttatatgag agccttctac ttctaatcct ggaaccatat attttggtga tatactatct
    40021 tttataatga aaacaccatc tatatctaac tcactctttc tcaatactaa tttattgact
    40081 agttttccac cttttatttc taaaggttta tcatctatta catttatacc actattgatt
    40141 ttattttcta atttatacat aggtatatag tatacattac ttgctatctc acttaaaaaa
    40201 tttgcctctt cttctgcttc atagttgtac cctattatag caactttttt atttcgatat
    40261 aacatagcat cacatgttgc acaataccca actccttttc caagatatgc gtcttctccc
    40321 tttatcattt ttccatattc aatacctgta gctaatacaa ctgtagttgc ttcatataca
    40381 tgatttccag aaactaatgt aaaatattca cccatactat atacattgtt tattctgttt
    40441 tgagttatct ttattcccat atcatctata tgatttttaa atttctcttt aagctctaaa
    40501 ccacttatat tactaattcc tagataattt tctacagaag gagcttttat aagtttttga
    40561 cttatatctt cagttccaaa taaaattatg tttttatttc taattttagc atttatagca
    40621 gctgcaagcc ctgctggacc acttcctatt atcgcaatat cgtatctcat aatctctcct
    40681 attttcaata acctttcctt ttttatctaa ataatatatc gaaagtcaat ctcattagca
    40741 attggatact tatttataac cacacaaact cttatactat ataaacaaaa aaactcatta
    40801 taatgagttt tttgtttata tatgatttat tttaattaca tatatgattc aactttgttc
    40861 tttaaattat ccttaggcat aaaacctatt aatctatcta caggttttcc atctttaaat
    40921 atcatcatag ttggcactgt ggatacttca aatttttgag ctagttctat actttggtct
    40981 atatctacct ttaaaaattt tgctttttca gccatttcat ctcctaaagc ttcatatatt
    41041 ggtgataaca tcttacaagg tccacaccaa gttgcaaaga aatctaccac tactaatcca
    41101 tctttatttt ctacttcgtt tataaattca ttatgattta ttatttttat cataatttac
    41161 atctccttta ttattttctt gatattaata tactacgacc tgtgtaatac ttcaattagt
    41221 cacttttttg taaccttcta tttttgctgg ctttaatatt atgataagca tatttataag
    41281 attaaatgtt tctaatatat tttaattttt tcattaatat agttgacatt ttttacttta
    41341 gattataaaa tcaaattaat aactacatag gtagttacta aacttttata taaggagttt
    41401 atctatgaat gatataatta atgaattaca aaatatagga tttactaaat acgaatctca
    41461 aatatacata tcattattaa aggaaagccc tttaacagga tatgagataa gtaaattatc
    41521 aggtgtacca caatcaaaag tctatgaaaa tataacaaag ctattaaaca ataatataat
    41581 tatcaatgtt ggaactgacc ctattaaata tgtacccata agtcctaatg aattattaaa
    41641 aaataaaaaa gaagaattta ataattctat ggataagtta aagaacaact tacaaatatt
    41701 aaataaaggt aaaaatatag aatacgtttt aaacataaag ggagataaaa atatattaaa
    41761 aaaagcaata gaaatgataa aaaatgcaaa acaagaaatt ataatttctt gcccttatga
    41821 agaaattctt gaattaaaaa aagaactcct aattgcttac aaaaaaaata ttaacattga
    41881 aatactactt ttagaaaata aaaatctcga aggtctagat aatattcata tccatggagg
    41941 taataaaact acacttgaaa ataaaatgct ttacaaagga attattttga ttgtagataa
    42001 taatgaaata ttgacaggaa acttatctga aggcaacgaa gccatttcaa tttggacaaa
    42061 aaactctaac attttatata ttggagttca atacataaaa catgaaattt atatttcaga
    42121 acaaataaaa ggaggttata aaaatgaaca agattaagat taaacaagtt gatgctttta
    42181 caactatacc ttttggtgga aaccctgctg gtgtagttac taatgcctct aatatttctg
    42241 atgaaatcaa gcagaatgtg gctaaagaaa tgaatttatc agaaactgca tttgtttcag
    42301 agtcaaatgt tgcagatttt aaggtacaat ttttcacacc aggtttagaa gttgatttat
    42361 gtggtcatgc aacaataggt actttccatg cacttttgga agaaggaaaa cttgattcta
    42421 ataagagtat attttatcaa gaaacaaaag ctggtgtatt atctgtagag cttaaggaaa
    42481 taaataatga aaatatattt atgatggagc agaatacacc tgaatttgaa aatttagata
    42541 attacagaaa agaacttgca caaatgatag gtgtacatga agatagactt cttgaatatc
    42601 ctattatgaa ggtaaatact ggactttggt ggcttgtgtt tggcttaaaa tctttagatg
    42661 atttaaaaaa tatctctcca gatttaaata aaatcaaaaa atttagtggt gataataata
    42721 taattggcat aaccccattt tgtatagaaa ctatagataa aaagtgtgac taccatttaa
    42781 gatctatcgc accatatgtt ggagttacag aagaccctgt atgtggaact ggaaatgctt
    42841 gtgtatcatc ttatatagta aatcaaaata ttttagacaa aactagtttt attggtgagg
    42901 aaggtaactt tataaacaga cctggaaaag tatatgtaaa tatagctaaa gaaaataatg
    42961 agataacttc tgtgaaaatt ggaggaagtg cttatactgt cttaagtggt gaaattatat
    43021 attaatctca tccctaataa aaacaaataa aaatactagc taactaatgc tagtattttt
    43081 atttgtttat ttttatttaa taataatact ctaatacata ttataatcac atatattata
    43141 acattatatt tagaagtaaa attttatttt tactattaat tagttataaa tcaatctaat
    43201 acaatataat tacaatagat taatccataa aacacaataa ttacttactg attatttatc
    43261 atcctcaaca agtctattta atgtcttgtc aaataagaat aataatatag ctgcaacaaa
    43321 tagtattact ggaattatag tatacacttt aatcatctca aatttaagag taaatgcata
    43381 tatatatccg tatccttgac cagctatgaa agtagcaaat gtccatactc ccataagaac
    43441 acctaggagt ttcttaggtg catacttact tacaaaagag ttaccaagtg gtgagaataa
    43501 catttcacca aaacttaata gtataccaaa catgattatc cacattatac tagctttagc
    43561 agtttcaggg gctccaacac ctcttgtaaa ctctgcccca acaagcatta aaaatgcaag
    43621 acctaagaat attaacccaa gacctgtctt tttaaataaa ctcatatctc cttgtggtct
    43681 actagcaagc ttaaaccata atgctccaag aactggtcct aatactatac atacaagcgc
    43741 atttaatgag tcaaaccaag caagaggaat atcaaatcct ccaatagtca tatctacaaa
    43801 tgctgcacca taatcataaa ctgcaagata agttagatac cagaataccc agaaaattac
    43861 agagaagaat gatataagta taatagccca aattctcttc ttatccatac ttgtaagagg
    43921 agacttatct ttttcttcaa ctttttcagc aactacacct tctttaaaag gtctctttcc
    43981 tgcatttcca aggaatctcc atccatatgt gaaccatatt gcacctatta cacaaagtac
    44041 agctgctata aagaaacatt gagaaaatcc aagcacacca ttttttgcaa aagtcttggc
    44101 ataaagtata ccaactgctg tagttccaat aaaagaacct atattaacaa aagaatactg
    44161 tactgaaaat gctgtatcaa gttcttcttg attatcaaaa agttgtccat ttactgcaga
    44221 cacatttcct ttaaagaatg cagtaccaat tgaaactaag attatcattg catttaccat
    44281 agaaactgta gttgcaatag aaccaagata gtatcctaca cccattataa gcatacctat
    44341 aggtatagta tacctagctc ctatccatct atctgatata taccccccaa ttataggtgc
    44401 aagatatgtg aatgcaacaa ggttagcttg cattatagct gcttgtccct tatctattcc
    44461 aagtccacca tgaattacag ctgttgtaag gaatatatag attaaaaact ttgcagaata
    44521 atatgctgct ctttcaaatg agaaagttgt tgagcagata taaaatccca taggaaattt
    44581 acccttcttt tttactgttg agctcataag tttaaatcct ttcttaatta tatttttata
    44641 tttttacgtt ttatccaaac tctttctttt aatgaaagat atggttatat tattttgtaa
    44701 aacaatttgt aattattatt aagataattg cgcatttatt tgtatagtat atattttcat
    44761 ggaataaaat taatatactt ctttagccat aaatttaaat tatataaatt acttgttttc
    44821 atatatttat aaattagtac cttaatcatc taataccatg aaaaaaactt cgccatatgc
    44881 aattcttatt tttttacgtg atagtatttt acaaactaaa atcccccttt tgtagcaact
    44941 tatgtaatta cattttagcg cttttttaaa ggagtctaga ataaagcaac atatatttat
    45001 tcaaataatg tttttattta cttttaaatt tatatattac ttcacccaac caattctcaa
    45061 cttaaataaa taaactacta tctattcaca gattatattt atcttttact tattagattt
    45121 ataataaaca ctgtgaatct aaacataaaa aactgttatt ttaaagtgat tacccatata
    45181 ttcgcttcga tactatttgt taaggttata ctatttattt ataatatatt attcataaaa
    45241 taccttaatc tgaaaacttt ttcacgaata tttattttac acctcccact atttatttca
    45301 ccatgtaaat cgtaccataa atatatactt atatgcaaga aaaaaatcta ttaatcgaca
    45361 gtaaataatt agaaaaattt aaattatatt tgtcaagtat attatataaa atattttcta
    45421 tgaaaatata agttaaatta atgttcagaa tagtttttta gatatgaaat cttgattttc
    45481 ttattacttt acttacttgc tcaaatatat ttttgtatgc agtatataat agtcaaatcg
    45541 tacacttctt ataactgcgt tgatttgaaa ataatattaa tagttaatag aagaaaaaat
    45601 tcaaattaat ttttaaaaaa actataagtt aattgaatac ttttttatgt acttaattag
    45661 ttttataaat tcaattattt tctaaattta ggtgtgattt accatataaa aatgattttt
    45721 tatcttaatt ttctaaatat aagtgaatgc tatcatttta agtatcttat aagcaatgta
    45781 actatatata tctaataact acttttttaa tatgcttaaa ctatttataa gcaattatat
    45841 ttatctgtta acctaataac tttttcacta aaaaataaca atctattcaa aaataaacta
    45901 tagtgcttct tctactttgt ttcaataaaa tactaaataa atttcgtaca ttcacaattt
    45961 ttcaggctac actataaata tacaatattt atcttagtta taagtctaag aatattttta
    46021 gtcttatttt gtcgaatatt tttaataaaa cttcataatt ctaagcatat tttttacact
    46081 gtgttttcat gtacaaaaat aatacttaca aataaacaac ataaataaaa tttaaataag
    46141 atggattcta ttcaacatag tatccatctt attcattcaa aacctaaaag gattttttat
    46201 aatattatac attttaaaat ctaaataact cattatagta tcaatttcac ttaaagctta
    46261 caatacactt attaatctta ctttataata aaaagtataa aaacatctac aataacaatt
    46321 taaatcttta agaaattata tcttatagta attcttcaat acaatcaccc ttaatttttt
    46381 atataattta attatatact ttattttact agaactaata cacataaata atacttaaaa
    46441 tagattaaat aaaggatact aaaacacttt attgaggtat atgacttgaa atatatgaac
    46501 taacttcatt aggagaaata tctaaaatag ttgcaaattc ttcatttaat aacctttcag
    46561 cttctttcat tatattatca tctgcttgat gtgttttttt accaatagat tttacatact
    46621 ctctgttatt gtctatgctt cttactaatt ttattaactc ttcacattta ccacctctga
    46681 gtattttttt aaattgctca cttcttaatt tttcattatc tatccatgag gtatccatat
    46741 taggaatatc atttattaat gaaagtacat taattttaga aagcaacttt ctcattggta
    46801 ctttttcatt atctacagga atttttatta ttgtattatt agagtaaata ggattcaata
    46861 cataatactc tttttttatt ccatttgtaa atcgttcatt tgttatatcc atgactttgc
    46921 agactcctgt cataccatac attatataat catcaatctt aaacaaaaac atcaccatcc
    46981 ttaataaaaa aaaaagtttt gacaactaaa tccaccttgc caaaactatc tgctatatat
    47041 tatatattaa cacattttaa gactttaatg taagtaaaac ttatattatt aaaattaaaa
    47101 aatacatcaa gcattgatta atgtattttt tagattttta taacaagcat atcaaaataa
    47161 attaagacag cttatctcct gtaaatacct ataaaattgg tgcttggcta ttcattgagt
    47221 acccatcatt tcttacatca tttatttcta atatttcatc atttgtttca actttagtaa
    47281 gttttttaac tgctccataa ggttctttta atgaaatttt atctcctctt ccagaaataa
    47341 cccataaaat tttataccct ctaggtataa cttgtagctt attttcacct ttaccatcag
    47401 taaagtagac taacaagtta atctttttat tattagcata ctcaaagact ggagaaaatc
    47461 tagtacctcc acctatccta attctatctt ttatatcttt ttcagttttc actttatata
    47521 cacgtctaat ttcattatca cattctataa ttataatttc atgattatag tttttcacta
    47581 tactaagaac ttctttaatc gcttgtttaa actcttcatc actaatactt ccacttatat
    47641 caagtgcaac tgctatttct gccttatgac ttctaagttc tcccctcaaa tctaaacgat
    47701 ttggttgtct tctatttctt cttgttatag tcttcttttt attattttca actgttccca
    47761 tcattctatt aagatataag ttccatggca attcactctt actattttta agtgatgata
    47821 tcatactttc taaataagca ggaactttac ctttttgaga actattaata aatttttcag
    47881 taaattctct aagggtattt tcatctatct cattagattc atcccatata tcatgagttc
    47941 tttctggact ataatcaatt tctatatctc catatttatc actatcatct tcctcaccat
    48001 cttcatccac ttccatcaaa tctaattcag tttgaatctt ttccacataa tattcaaaag
    48061 gttcatatgg ctcaagtttt aaggaatatt ttaaatttac ccactctaag gtagttgcat
    48121 atggtggtaa attgtttaga aatttattta ctactatatc cattgccata ttaattgcca
    48181 atgtactata ctttcctttt aactcctttg ctctcatcag atgcatagat aatatatgat
    48241 gaatttcatg tttaattgta gtctccattt gttttatatc aagcgttaaa aaaattattg
    48301 gattaaaata tataacatat ttagccccct taaaatttac acctgtagga ctacttatat
    48361 caaatcttat ctctcttaac atctgaaaca aaaaatatcc ataaaaatta tcctcttctt
    48421 ccataagact taaattcact ttatctacaa gactaaaaaa ctcatactta aaatcctgtg
    48481 gtatatctat atcaaatttc tcattagcac gatttgcctt taacatagaa tgagtattta
    48541 taatttcact tgctttatca taaagctctt ttgcttgttt atcaaaataa ttttccatac
    48601 attatcccct tattaaacta taagattcaa aatatgattt tacaaactct tcattttcta
    48661 tagctttttt atacacttca gtataactgt ttctaatatc cttcataata cctatcatta
    48721 agtccacagg atacattttt aaaaatttaa taaatctatt aatatagtga tttgattcat
    48781 atttatcatt cttaatattt agctctaaat cttttagaat attcatagct gatagataaa
    48841 gtcttgtatg actctctttt tttactcttt ctacaataga ctcatcaata gattctccta
    48901 aaaaaacttc ttcataggat attaatggtt tagaatctga ttcaataaaa cttataaatt
    48961 cttctgcgat aaccttacct acattaccct ttataacatt taaaaatact gctctaggaa
    49021 tagaattatt attttcttta taaactttat aaattttaga tactctttca taacttctag
    49081 gagtagctct tacatcatct tcatttattt tatgcagata ttctggaaaa gtggaaataa
    49141 actcgataac ttttctttca atcccttcat ctattgccca ttttatccat tgtgtatggt
    49201 cactctccat atttaaccaa acaaatctat tttcttgagc tgcatccata tctaccactt
    49261 gataatcaaa atctgaacca tatttacttg atgggttcat tgccgctaaa atctttacat
    49321 catcatgcaa tttatatcca ttaatctctc tatttaaaat tagattcata agttcttgct
    49381 gtactgtatg ctcacaacga tttatctcat ctataaataa aagaactatc ttacctttag
    49441 atatctcttc atcaatttcc cttaatttgt tatgaacagc atatacggtt gttttctttt
    49501 cagtcttata tccactagaa ttaactccta cataagattc tatagtagga agacctccta
    49561 tttcaccctc tttaagtagg tttccatcaa tagcaactaa actccaatca ttttctttgg
    49621 caagcttatt tgccaaagct gtttttccta tcccactttc acctactatt aaaggtactt
    49681 ctccagtgga taatattaag tcaacgcttt ttaatgtgtc tataaaattc atttttatct
    49741 ctcctaaaca atctttaatt tctcatctac agctattttt tttgccgttt tgcttccata
    49801 tttataaata aacataattt tatctattgg tatattctca ctatgtctat ctataacact
    49861 acaatttaaa aaatctctaa tatacttctc ttcaacatct tcttttgata atacttcttt
    49921 taatacttcc tccaattcat ctttagatat tttctttaca atgtatttaa tcactaagat
    49981 attcactttt aaaaattcca acattttatt cttatctaaa tcagttataa agctaatagc
    50041 tgcttcatta ttttttatag ctataagttc tgcactgtaa ctaggtgaat ctatatatct
    50101 caaggcatca taacttattc tcacagcctc ctcttgtaca ctttcataag gttgttttat
    50161 aaactttatc gaatcataat tttttcttac tgctaataac tgtaactctt cacttggatt
    50221 tttaacatat ttaattgccc aacctgcttg atttatagcc atttcaataa ctttatcagt
    50281 tggatttttg atataatcca gtatactcca tccctcattt actgctttta tcatcatctc
    50341 ttctgttgga ttatctataa atttaattgc tctaatatta ttatctattg ctagttcttg
    50401 catctctcta gttggattat ttatatattc tagtacaagt ccattttctt taatagctaa
    50461 taatttcatt tcatcagttg gattatctat agttgatata atattaggat tattttttat
    50521 catttcaata actcttgatt tttccataac atgcctttct ttatataatt atttttctct
    50581 ttatactaat tataataatt tattcctatt tatataattg tttcttatta taattaatat
    50641 actttttaag caaatttaaa cagaataatt ataccaatat ataaaaaaat ttctagaaat
    50701 caataaaatt aagtctatat acataaagac atctttaata tttataaagt cagacagtaa
    50761 tttaatactt ttattgacaa aaaaagacaa tccataaaaa attgtctttt ttcaacaaaa
    50821 ttcatcaatg taatttctag atatcgttgc tatccccata taattattga aattactaag
    50881 atatagtatt ttgttattta aatataaact tattctaaca tctatttatt tagatgtttt
    50941 tccataatat ctaaaattat ctaatctatt gtaatactta ctttttgaga tgatattgtt
    51001 gcattttcat cttcaacttc agcccaaaaa gtatgagtac ctactattaa tgaataaagt
    51061 acaaatgatt tctttacttc attaggattt ctaacactta gttcttcatt tacaattttt
    51121 tgcccatcca tatatacatt taaagttttt gcattattca ttgctggaac gattacctca
    51181 acatcaactt tattaggatt aggtgatatc ttaattgatg gtggtattat actatctact
    51241 attatattta cttcttcaga ttttaaacat ttaccattgc catcttcaag aactgcaaaa
    51301 tatgtataaa aaccttcttt tttgtcactc atatcaaaat taatattttg taattctgaa
    51361 cttcttacta aatcttttgt ttctatagga acattattct caaatagttc caatttagtc
    51421 gcagaattat taggaggaac agaaacatta atattaaatt taccattatt actcaaggtt
    51481 ttatcagttg atatttctgg agactgtaaa tataattctg atgaattatc gatttgaccc
    51541 aaatcgaaca ttttcgaatc accattaatt tcaacatttt tggagattaa ttcgggagat
    51601 agtttatgcc ccttattatc tactaggata attgatttaa taattgctac tttatcatct
    51661 tttaaattat cctttatctg taaataaatt ttgtctgcac ttgataggct aacattaagt
    51721 tctttctcta aatgtgctaa atcaattaca aaagtgattt ctgaaccatt atttacacca
    51781 tccaatggaa aatctcctcc actataagat aataatggac tgtatactac tgtttgttta
    51841 ttaccatttt tctcatatac tagacttact tgcaactgtt ttctgttatc atgttgcata
    51901 gtgaatctca ctaatttgga tggtgtttct tttttgctta ttactacata ttcagcttca
    51961 ttatttaaga atgctccttg tcttgcatta ctatttgtaa agtctaaaac tgatgatttt
    52021 ttatttaatg catcataagc catccaaaca aatccattgt ttgcatagtc aggtccatga
    52081 gaattagcaa tcttaaaagc acctttttca ccatcatcaa ccttaccatt gctattgata
    52141 tctatccata tattgtcatt gtatcctaca atagtcattt catgtgcacc ttgattatta
    52201 atttgataaa ttgctttctg atttacataa ctatcatcgt ctttagtcga ggggtcatta
    52261 gaaatttgtt tataattagc atctgagaac caagtataga aatttaaaat atatccattt
    52321 agtaataatc tcttcgcatc atccaagttt gagtctttgt ttgatgtaat aaaagaattt
    52381 ggcttattat ttttattaaa attaaccctt aatatttcat ctacctgata tttagttgca
    52441 tttctccaga tattttcatc tttagcccat tccctataat ttttaggatc tctgcctaaa
    52501 cctacataag gaaagtcagc taatgtagca caaccatgtg ttttaaaaac attataattc
    52561 tgataagcaa atgctcctgc atctttgcca tcgtttatga aattataaga ccacttaggt
    52621 gaaaatattt tagaatctat attttttcca ctcacatctt ttacactcca atcatttaca
    52681 agagcagtca tataagtcat agtatagtat gctgttgcat aggatgcaca gcttgccaaa
    52741 gaaccttggc tccctatgtt aggaaaatac ttagtttggc tattgtcaac atttgatgga
    52801 agtttaatat tttctgaatt gtcttcaagt gaaaaattag aagtgtctcc ttgtttaact
    52861 atttcatctc ctaattttgc tggagataca ttttcaataa tttgttttga gttgatattc
    52921 ttaattcttt ctaatgcaat actatttaaa ttaacctttt ctgttaattt agaaatttcc
    52981 agttgcttag ctctatcatt gtcagtaaaa tctggtaacc ataatttctc ttctgaattg
    53041 taattatttt cataggcatg caccaaaaat ttttgattaa atgttaaagt tgtaattgtt
    53101 gctagtaata atgctattct tttcttttta attttcttca aattaatccc cccaatttta
    53161 ctatacattt ttgttaaaat ttattttaca acacaatttt aattatttca ataaaaatat
    53221 a
//
LOCUS       DAFNNB010000007        51266 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0007, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000007 DAFNNB010000000
VERSION     DAFNNB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 51266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51266
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0007"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..388
                     /locus_tag="L3M60_000368"
     CDS             <1..388
                     /locus_tag="L3M60_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBN6225075.1"
                     /translation="RQFSGGYHANSNKNCILIFNSIFFISMVLGIILNDSNPIPLFLV
                     ILSSFNFLSIFIFAPVCHANNPLNETKYKKNKLMSRIISITLFLIILLGCKYLSFYNY
                     VVYTFLALLWINLLLIIQIIINKKII"
     gene            401..535
                     /locus_tag="L3M60_000369"
     CDS             401..535
                     /locus_tag="L3M60_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBN6225076.1"
                     /translation="MKKILYHFMKACGVLAMFVAFLSANTTSLWHVYQPKTPEKLKKF
                     "
     gene            complement(729..2294)
                     /locus_tag="L3M60_000370"
                     /pseudo
     CDS             complement(729..2294)
                     /locus_tag="L3M60_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013280575.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            2742..3563
                     /locus_tag="L3M60_000371"
                     /pseudo
     CDS             2742..3563
                     /locus_tag="L3M60_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003520701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(3588..4208)
                     /locus_tag="L3M60_000372"
     CDS             complement(3588..4208)
                     /locus_tag="L3M60_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6225077.1"
                     /translation="MSDNLVNDTNSTRLGGIDRYDTNKKIVNKFYNGAKEFYIASGTD
                     LVYALVGSTIAKNDPIILVDTNSDKNILEGSSKITAISIENSSIIQQCIDVSNGIKKY
                     GWIKLGDSWHYYNKDGTEVSNYDLALKKANKVLGDKYEAYLIENTDSLPNFAQTYLDE
                     CYFFGITYKIDDSDSAILVNKKNFYAFQIDSVGRISELEKYSDPSF"
     gene            4247..4375
                     /locus_tag="L3M60_000373"
                     /pseudo
     CDS             4247..4375
                     /locus_tag="L3M60_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4457..5818)
                     /gene="rlmD"
                     /locus_tag="L3M60_000374"
     CDS             complement(4457..5818)
                     /gene="rlmD"
                     /locus_tag="L3M60_000374"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBN6225078.1"
                     /translation="MSVKKKDVIEFEVDKMEFGGTSLSQVGDRVIHMKGGISGQKVRA
                     GVKKVRSKKAEVKMIELLENSPLETEEACKHFRECGGCTLLSVPYEKQLEIKEKQVMD
                     LFLKQDLFGFQFLGIEGSPENKYYRNKMEYTFGDEVKNGPLTLGLHKKGKHIDIQTVE
                     ECMLVDEDFSKILVSSVEFFNEKKLPYYRTMNHKGYLRHLVVRKGIHTNEIMVNIVTS
                     SQEDFDMNEFKDMLLGIDLKSELVGVLHTINDGLADAVQCDELRVLYGRDYIQEEILG
                     LKFKISPFSFFQTNTKGAEVLYSIARDFIGDYNDKVVFDLYSGTGSIGQVMAGAAKKV
                     YGIEIVEEAVVAANENAKLNGLTNCEFIAGDVAKVVKDLKDKPDLIIVDPPRPGIHKD
                     AIRDICGFGAKEIVYISCNPKSLVVDLVDFKRYGYEIRMVKCMDMFPNTPHCETIVKL
                     VRE"
     gene            complement(5845..6567)
                     /locus_tag="L3M60_000375"
     CDS             complement(5845..6567)
                     /locus_tag="L3M60_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBN6225079.1"
                     /translation="MAKKQRIDKILSNLGYGSRSEIKKYCKQGSVVVNGSEVSNPGTQ
                     VDTENDEILFNGEEVIYREYIYLMMNKPDGYVSATTDKYDPTVLDLIDLSYLAFEPFP
                     VGRLDKDTEGLLVLTNDGKLSHRVLSPKKHVPKTYYAKIDGVVTEEDVEAFLEGVVLD
                     DGYKTMPSQLNILKSDDESEIELTIHEGKFHQVKRMFESVGKKVVYLKRLSMGNLKLD
                     ESLELGEYRELTDEEIKLIEER"
     gene            complement(6567..6896)
                     /locus_tag="L3M60_000376"
     CDS             complement(6567..6896)
                     /locus_tag="L3M60_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1904 domain-containing protein"
                     /protein_id="HBN6225080.1"
                     /translation="MPQIKIRGINEDDICKISEKMIDDLVEAVKCPRDYFEIECIKSV
                     AIRDGKIADVYPFVEIAWFDRGQEVQDTVARIITDSIRTNLDVESMDLAFTVFEKEKY
                     YENGEHF"
     gene            7226..8152
                     /gene="fba"
                     /locus_tag="L3M60_000377"
     CDS             7226..8152
                     /gene="fba"
                     /locus_tag="L3M60_000377"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-1,6-bisphosphate aldolase"
                     /protein_id="HBN6225081.1"
                     /translation="MALITTKEMFKKAYEGGFAIGAFNISDLEQLQGVLKAAKAKNSY
                     VMIQASMSAVKYAGPHTLVEMVKAASDEIGVDVALHLDHGPNMDAIKTCIDAGFSSVM
                     IDGSHFDFEENVRITKEAVEYAHSKGVVVEAELGVLAGTEDDVTSDVHKYTQPAEAVE
                     FVERTGVDSLAIAIGTSHGAFKFKGEAKLRFDILEEIQSKLPGFPIVLHGASAVDQNA
                     VATCNEFGGNIAGAKGVPVDMLRKASSMAVCKINMDTDLRLAMTAAIRKFLAENPKEF
                     DPRKYIGAGRDAIQAVVESKIDDVLGSANSIN"
     gene            complement(8767..10398)
                     /locus_tag="L3M60_000378"
     CDS             complement(8767..10398)
                     /locus_tag="L3M60_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6225082.1"
                     /translation="MDNNLIGLIEHVENPAILCKESGEIIYCNHLIDSIFSFLDIKKP
                     KNINELDSNFDKTEILTDSKKKIAFRELRMTAHIYNMKDNNNKNNVVYLFEKSLISDK
                     VIEDIIEHIDEVVVVFNKDGVIEKMNTVSDEILPFKRTEVLGRNITDLVRQGLVEEPI
                     ILNMLKVKKKIYRNIVYPDGKLIAYTAVPRWDSKGKLKGGVLTGRDISRVIKLESQIK
                     YSDISEDTEYISQSKIMDNIKKVVKRAAASDSSIFINGESGVGKEIIARTIYKYSSRR
                     DKPFIAINCGAIPNELLESEFFGYEEGSFTGAKKKGKKGLFEEANGGTIFLDEIGELP
                     MQMQKKLLRVIQENTITRIGGSKPIKIDVRYISATNISHEDLRNNLKFRQDLYYRLSV
                     IPVKIPPLRERKEDIVPLVNYFLKLYNEKYNREVEVSPKVIELLEEYSWPGNIRELKN
                     IIERFVVLSAKNIIGEDEFNMLINLDMIDNETDDLSPIVVNGIMNLNDAYKIVDQIMI
                     SKAINKYGSITKAAEVIGIYPSTIHRKIKSGHIHV"
     gene            complement(10880..11917)
                     /locus_tag="L3M60_000379"
     CDS             complement(10880..11917)
                     /locus_tag="L3M60_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBN6225083.1"
                     /translation="MNDIKDLSSYKNVWIFAEQREGKIAPVVIELLGEGRKLAKEVDA
                     ELCAILLGKDVDGLAKELITFGADKVYVADDALLEKYTTDAYTKVIKDAIDEIKPEIM
                     LFGATHIGRDLAPRIASRVGTGLTADCTKLEIDPEDKKIKQTRPAFGGNIMATIICPN
                     HRPQMSTVRPGVMDKAEKDETRTGEVIALDYKITQDDIRTTVLETVKTKKDLVSLTDA
                     NVIVSGGLGLGGPEGFEMLKKLADKLGGVVGSSRAAVDAGWIDHSHQVGQTGTTVKPN
                     LYIACGISGAIQHLAGMQSSDFIIAINKNPAAPILEIADYGVVGDLHEIVPMLIEKLD
                     SVDDLLEAIKA"
     gene            complement(11936..12721)
                     /locus_tag="L3M60_000380"
     CDS             complement(11936..12721)
                     /locus_tag="L3M60_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012703997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBN6225084.1"
                     /translation="MKILVCVKQVPDTNEVRINKETGTLIRDGVPSILNPDDANALEE
                     ALKIKDEYDDVTITVITMGPPQADFMLRECLAMGADDAILLSDRAFGGADTWATSNTI
                     AAGISKIGDYDIIFAGRQAIDGDTAQVGPQIAEKLDIPQVTYVQDFKIEGKDIIVQRQ
                     LEDGYELIKVSTPVLLTAVKELNTPRYMSVDKIVKAYKHDVKVWTIDDLDVNKEEVGL
                     KASPTKVFRSFTPEPRGKGEILEGKADEIASKIIIGLKQKHII"
     gene            complement(12754..13887)
                     /locus_tag="L3M60_000381"
     CDS             complement(12754..13887)
                     /locus_tag="L3M60_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBN6225085.1"
                     /translation="MLYNKEQELLRKAVRDFVSKELDTLPAEMDKTGVMPKELIKKLA
                     DAKFISSNIPEEYGGGGAGYVSYAIVMEEIARRCASTATFVTAGSSLASLPILYNGTE
                     EQKQKYLKGIATGELIGAFGLTEPGAGSDAAGQQTTAELVGDHYILNGRKTFITNGPF
                     CDVAIVIAVTDRSKGLRGTSAFIVESKWDGFSTGAHEDKMGIRGTETSDLIFENVKVP
                     KENLLGKEGQGFKIAMGTLEVGRIGVAALALGIAQGALDEAVKYTKQRVQFGKPIAKF
                     QNTQFTIADMETKVCAARGLVYDAAQKRDAGMRVAQESAMAKYYASEIANEVAYKALQ
                     LHGGYGFIKDYEIERMYRDARIVSIYEGTSEVQKMVISSNVLK"
     gene            complement(13935..15062)
                     /gene="hadC"
                     /locus_tag="L3M60_000382"
     CDS             complement(13935..15062)
                     /gene="hadC"
                     /locus_tag="L3M60_000382"
                     /EC_number="4.2.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     beta"
                     /protein_id="HBN6225086.1"
                     /translation="MEAILSKMKEVVENPNAAVKKYKSETGKKAIGCFPVYCPEEIIH
                     AAGMLPVGIWGGQTELDLAKQYFPAFACSIMQSCLEYGLKGAYDELSGVIIPGMCDTL
                     ICLGQNWKSAVPHIKYISLVHPQNRKLEAGVKYLISEYKGVKRELEEICGYEIEEAKI
                     HESIEVYNEHRKTMRDFVEVAYKHSNTIKPSIRSLVIKSGFFMRKEEHTKLVKDLIAK
                     LNAMPEEVCSGKKVLLTGILADSKDILDILEDNNISVVADDLAQETRQFRTDVPAGDD
                     ALERLARQWSNIEGCSLAYDPKKKRGSLIVDEVKKKDIDGVIFCMMKFCDPEEYDYPL
                     VRKDIEDSGIPTLYVEIDQQTQNNEQARTRIQTFAEMMSLA"
     gene            complement(15062..16288)
                     /gene="hadB"
                     /locus_tag="L3M60_000383"
     CDS             complement(15062..16288)
                     /gene="hadB"
                     /locus_tag="L3M60_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(R)-2-hydroxyisocaproyl-CoA dehydratase subunit
                     HadB"
                     /protein_id="HBN6225087.1"
                     /translation="MSEKKEARVVINDLLAEQYANAFKAKEEGRPVGWSTSVFPQELA
                     EVFDLNVLYPENQAAGVAAKKGSLELCEMAESKGYSIDLCAYARTNFGLLENGGCEAL
                     DMPAPDFLLCCNNICNQVIKWYENISRELDIPLIMIDTTFNNEDEVTQSRIDYIKAQF
                     EEAIKQLEIISGKKFDPKKFEEVMKISAENGRLWKYSMSLPADSSPSPMNGFDLFTYM
                     AVIVCARGKKETTEAFKLLIEELEDNMKTGKSSFRGEEKYRIMMEGIPCWPYIGYKMK
                     TLAKFGVNMTGSVYPHAWALQYEVNDLDGMAVAYSTMFNNVNLDRMTKYRVDSLIEGK
                     CDGAFYHMNRSCKLMSLIQYEMQRRAAEETGLPYAGFDGDQADPRAFTNAQFETRIQG
                     LVEVMEERKKLNRGEI"
     gene            complement(16303..17103)
                     /gene="hadI"
                     /locus_tag="L3M60_000384"
     CDS             complement(16303..17103)
                     /gene="hadI"
                     /locus_tag="L3M60_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyisocaproyl-CoA dehydratase activator
                     HadI"
                     /protein_id="HBN6225088.1"
                     /translation="MYTMGLDIGSTASKGVILKNGEDIVASETISSGTGTTGPSRVLE
                     KLYGKTGLAREDIKKVVVTGYGRMNYSDADKQISELSCHARGVNFIIPETRTIIDIGG
                     QDAKVLKLDNNGRLLNFLMNDKCAAGTGRFLDVMAKIIEVDVSELGSISMNSQNEVSI
                     SSTCTVFAESEVISHLSENAKIEDIVAGIHTSVAKRVSSLVKRIGVQRNVVMVGGVAR
                     NSGIVRAMAREINTEIIVPDIPQLTGALGAALYAFDEAKESQKEVKNI"
     gene            complement(17142..18341)
                     /gene="hadA"
                     /locus_tag="L3M60_000385"
     CDS             complement(17142..18341)
                     /gene="hadA"
                     /locus_tag="L3M60_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocaprenoyl-CoA:2-hydroxyisocaproate
                     CoA-transferase HadA"
                     /protein_id="HBN6225089.1"
                     /translation="MLLEGVKVVELSSFIAAPCCAKMLGDWGAEVIKIEPIEGDGIRV
                     MGGTFKSPASDDENPMFELENGNKKGVSINVKSKEGVEILHKLLSEADIFVTNVRVQA
                     LEKMGIAYDQIKDKYPGLIFSQILGYGEKGPLKDKPGFDYTAYFARGGVSQSVMEKGT
                     SPANTAAGFGDHYAGLALAAGSLAALHKKGQTGKGERVTVSLFHTAIYGMGTMITTAQ
                     YGNEMPLSRENPNSPLMTTYKCKDGRWIQLALIQYNKWLGKFCKVINREYILEDDRYN
                     NIDSMVNHVEDLVKIVGEAMLEKTLDEWSALLEEADLPFEKIQSCEDLLDDEQAWAND
                     FLFKKTYDSGNTGVLVNTPVMFRNEGIKEYTPAPKVGQHTVEVLKSLGYDEEKINNFK
                     DSKVVRY"
     gene            19035..20033
                     /locus_tag="L3M60_000386"
     CDS             19035..20033
                     /locus_tag="L3M60_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBN6225090.1"
                     /translation="MKILVFGARDYEEPVIKKWSEEHKDVQVDIYPENMTEENIVKAK
                     GYDGISIQQTNYIDNPYIYETLKDAGVKVIASRTAGVDMIHFDLVNENGLIVTNVPSY
                     SPNAIAELAVTQAMNLLRKTPLVKKKVCEGDYRWIAELLGTEVRSITVGVIGTGKIGA
                     TSAKLFKGLGANVIAFDQYPNSDLNDILTYKDSLEDLLKEADLITLHTPLLEGTKHMI
                     NKDTLAIMKDGAYIVNTGRGGLIKTEDLIEALESGKIRAAALDTFETEGLFLNKKMNP
                     GELTDPEINKLLSMEQVIFTHHLGFFTSTAIENIVYSSLSSAVEVIKTGTATNRVN"
     gene            20407..20934
                     /locus_tag="L3M60_000387"
     CDS             20407..20934
                     /locus_tag="L3M60_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04002 family protein"
                     /protein_id="HBN6225091.1"
                     /translation="MEKMNKVTSKKTTYIVTSALFASIICLTIAYILHIPVGGNNGYV
                     HIGDAFIYLAATILPTNYAIAASAIGAGLADLSTGAAIWVIPTIIIKPILVLFFTSKS
                     DKIINKRNIVASIVAGIIGLVLYMFAEGIIIGSFTSAFVMSLLGLLQPIGSFIVFIIL
                     GMALDKLDFKKRYFN"
     gene            20961..21557
                     /locus_tag="L3M60_000388"
     CDS             20961..21557
                     /locus_tag="L3M60_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04100 family radical SAM protein"
                     /protein_id="HBN6225092.1"
                     /translation="MNILYTIENSIYVNITNTCPCSCVFCIRNEKDEVANSGSLWLEH
                     EPSVDEVKEAFNKYNLDDYDEIVFCGYGEPLMRINELIEVAMFIKEKSSIKIRINTNG
                     LSDLIHNKKTAIMLKDVIDAVSISLNAPNKEAYNRVTQPKFGEKSFDYMLDFARDCKK
                     YIKEVAFSVVDEISPKEIEESKQLAKKLDIPLRVRHKN"
     gene            complement(21622..23205)
                     /gene="asnB"
                     /locus_tag="L3M60_000389"
     CDS             complement(21622..23205)
                     /gene="asnB"
                     /locus_tag="L3M60_000389"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBN6225093.1"
                     /translation="MINLEEKYSTRKDTCMIKILEYNGHIRNWKVLCEELGIDSSLGR
                     DERERAILVEAYKTWGYDMANHMHGMFAFALWDTVEKKLFCLRDQFGVKPFYYYKTEN
                     GKLLYSSSIRKLMEQPGFVKELNEEMLQMYMSFTYVAGENTFFRGVKKLMPGHYLVYQ
                     NKTVCIERYWKPEFHPDYSKSIEEWTDEIHTTLQKIMPEIKSEDETAELFLSGGVDSS
                     YILAMSDIEKTGSCGYEEEWFDESKLAQQTTDILGCKNSRYIITPEEYFASVPYVMYH
                     MEQPTGDASAIVFAIGCKAAAENTNICYSGEGADEFFGGYNVYHKAKCYGENLKTFYI
                     GNTNIMKEDEKRKILKKYNPDVLPIKLTKRIYEETEELDTLTNMSNIDIQIWLEGDIY
                     FNIDKMSTAVGLEVRMPLTDTRIFDIASRMPSEYKVKGGENKIAFRKAASKVLPEEIA
                     FRNKLGFIVPIRIWMADDKYNQDIRDKFNSEIADKFFNIDEINEIFNDYISGNSNNWR
                     KIWTIYTFLVWYEIYFVKC"
     gene            complement(23772..24608)
                     /locus_tag="L3M60_000390"
     CDS             complement(23772..24608)
                     /locus_tag="L3M60_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6225094.1"
                     /translation="MNYIIKKVLNSSVVLVHDANDNEFILLGKGIGYGKKTGEYICGS
                     NDNQMFVPVENTKSKQFLELMDDISVDILKITQEIIVEAEKLLNSSFNKNLYLILADH
                     FNFAIERMRKGIKITNRVFWEIKNYYPNEFKVGMVAIYMVEERLGIQLPEEEAANIAF
                     HFANAMASDGNSYDTIKYAKVIGEIINIFVFSLNRTLDKKSMHYMRFITHIKFFVERF
                     FSDSMLSSVDDLLFNQMKRSNPKEMSIALKVRDFLEKKYGKKLTNEEIAFLVVHIARV
                     KQ"
     gene            complement(24632..26098)
                     /locus_tag="L3M60_000391"
     CDS             complement(24632..26098)
                     /locus_tag="L3M60_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBN6225095.1"
                     /translation="MRKFPEGFLWGGATAANQFEGGWNLGSKGWSVSDVAKAHFDVDV
                     KDYKSNNEITTRDIEEGLAHPEDEVNYPKRHGSDFYHHYKEDIALMAEMGFKTYRMSI
                     AWSRIFPNGDDTEPNEEGLQFYDDVFDELISYGIEPLVTMSHYEPPLNIVLNYDGWYS
                     RQVINMFVRYVETICERYKNKVKYWLTFNEVDSMIRHPYTTGGLVRDRFKDKNFEGVI
                     FQAMHHQFVASALATKICHEIIPNSKVGCMLTKLTYYPYTCRPEDVLAAQQKMRSIYA
                     YSDTQVFGEYPVYLLSYFKNNNIQIVKEEQDDEIMKKYPVDFISFSYYMSSCEAADTT
                     GLDVTSGNTLLAVKNPYLEMSEWGWQIDSIGLRISLIELYDRYRKPLFIVENGLGAKD
                     ILTEDKKVHDQYRIDYLKEHFKCMLDAIIEDGVELWGYTSWGCIDLVSESTKQMSKRY
                     GYIYVDADDYGKGTYNRYKKDSFYWYKKVIENNSIDFK"
     gene            complement(26101..27963)
                     /locus_tag="L3M60_000392"
     CDS             complement(26101..27963)
                     /locus_tag="L3M60_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6225096.1"
                     /translation="MDYKKTAIDIIEQVGGSKNILALEHCSTRLRFKLIDRSKVNQDA
                     LKKVQGVMSIVDRAQFQVVIGNSVVDVYDEILKVCPLDTNDSAIKTEEKINLGTRFIE
                     FIINIFQPLVFAIAGAGILKSLLMLLAMFNILKSDSTIYTLLIAISDATFYFLPLMVA
                     VTTANVLKCNRLVAIAAVGYLLLPATTTALSEGVELFGFTIPSIAYNAQVFPAILCVS
                     FLSIMEKIFNKYSPKPIRTFFVPMMSLAITVPITLTILGPLGYNVGTIFTTIILFLYN
                     KMGFLVVGLLAAVLPFMVATGMHKALTPYAINTIGKLGYEALYMTASLAHNISESGVC
                     FAVALRTKDETLKQTALSAGISALMGITEPALYGITLQHKRAILGVVLSSAISGIFLG
                     LFTVKGFVIVGPGLASMSMFIDPNNGNNLIFASIGFVIAILGSFIITLIIWKEDTNIE
                     QSSIENVVEEEPIEGKEVNILVSPVEGKVIDLSKVSDELFASKTLGEGVAIIPTNGNL
                     YAPCDSEVVMLFETKHAIGLRTKNGAEILIHIGINTVSMNGDGFKTFVKTGDNVKEGD
                     LLIQFDLDKISHANLDSTVMIVNSNGADYDYKVLNQSYGNVTKGSILFDVKGGI"
     gene            complement(28210..29073)
                     /locus_tag="L3M60_000393"
     CDS             complement(28210..29073)
                     /locus_tag="L3M60_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBN6225097.1"
                     /translation="MILSYFALLIRVVLLAFERVVVRLLGDEEGDIYKNIASSFLFFF
                     IGGVCLLPFSIMDKVSDWSFLIPCYISSLVYSIGSVAYVTSLATGEVSLVTPINSLNS
                     LFLLLLSVIFLGEGLSLAKVAGIIIMIGGVFILKKVSSFVKNVSVIFNNLPCRLMFLY
                     IFLQSVGRVLDKAFFVQVSPILYSTILYFFVGLNLFIFLAFKKKQKVIWEIFNNKKGL
                     SILSGAINGYSYLALLIALNNLELSIAEPFSQVSMIITLILAHFIFKENIKEKIPGSI
                     LILIGGWLLLL"
     gene            complement(29128..30060)
                     /locus_tag="L3M60_000394"
     CDS             complement(29128..30060)
                     /locus_tag="L3M60_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HBN6225098.1"
                     /translation="MNNKKAVFFFCSDLKKDPVASNVIKCCEEIMDLNQTDIVIDDNF
                     VLEFKDKNNNLFHFVKTKYVISHDYEYYLPILNRHFRDYDFAGVVNWHEGENAPEHIL
                     TAHTIGDVPTGEFGNSNPRYFKNLINAIEDIKNENSLDEFTTLTEATHWSGTTYGGES
                     KLITEYSVPMLDIEIGSSSDSFNNSIAIQVLAKSLIRVFDCDEHLKTLLCVGGVHFEK
                     SFSDIIKNKEFNISIGHVLPNQWIVSGMYDDESGLEKLEKCINSIADGIDGIVFHDKL
                     KGTYKEQCRKLGEKLNIPVFKHKILKNPKDLPIW"
     gene            complement(30146..30697)
                     /locus_tag="L3M60_000395"
     CDS             complement(30146..30697)
                     /locus_tag="L3M60_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6225099.1"
                     /translation="MQSLNEIIAENLKKIRKEKHLSLDKIAQLSGVSKSMLSQIEKCE
                     VNPTISTLKKITNGLKISFTSLMERQESDIELIQKSDVDHFIEDDGKYISYPIFPFDS
                     KRRFEIFMIEIEEGGNLDSNAELPGTQEFITVFSGEVTIKINGEDYVVSSGNSIRFKA
                     DVSHIYRNSGKGIAKMSMVVYYV"
     gene            complement(30922..31413)
                     /locus_tag="L3M60_000396"
     CDS             complement(30922..31413)
                     /locus_tag="L3M60_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225100.1"
                     /translation="MIDLKIENRKGKIHVKSSEIKDILELRPDFEYVQDISNTINQEN
                     ILVFDCQLTGNSFDMNDLDIEEILEELGEEIDESYFSVLFEDVRAYLKDATDEIEADL
                     QDNYLFDNLRCYFDIYNINQEFTDFKFVFLVSFKDIKISSLANLAKIVSKRQLIGASK
                     FYS"
     gene            complement(31441..32001)
                     /locus_tag="L3M60_000397"
     CDS             complement(31441..32001)
                     /locus_tag="L3M60_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBN6225101.1"
                     /translation="MNKLNICIDIDGTITSPYHFLPYLNDIFNKNITEAECITHNWEE
                     LYGSGIRDVYAEFNNKYIHSYDEAKIVEGATEVISTLSKGHNLSFVTARHECLTDVTK
                     NWLSRQGFSDIDVYLLGSDYKVEKARELSCDIFIEDNPLNSVQLADDGVKVILLDTNY
                     NKDVKHQNIVRVNNWIDIEKIIKQGI"
     gene            complement(32153..33310)
                     /locus_tag="L3M60_000398"
     CDS             complement(32153..33310)
                     /locus_tag="L3M60_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBN6225102.1"
                     /translation="MNKQSIGKLNMFFMGITQRNLDNRDFFIELEVIFKSGRKEFKGI
                     AIEEDNKYKFNFKGKSDLYTFNELLKNISKEAENYDGLVFKYVERGTIVLIEGDNKKV
                     NVKYLDNKEEVPKIDEFTASQIKNRDYYVKVGQANALLKEIGVLTKDGKIKNDKIRKY
                     NQIDHFVELIDNILKEIKDKDCITILDCACGKSYLSFVLNFYIKEVLKKNCYFIGIDY
                     SDVVIEASRNMAKNLGYKNMSFIKEDLTNYTPNRDVDLVISLHACDTATDMAIGLGIR
                     AKSEAIVVVPCCHKELLGQYRYEAMEPILKHGVFKARFADLITDGLRTLLLEGNGYDT
                     SVVEYISPLDTPKNLMIRAIKSRTNNDKALKEYKELKSQFGVEPTLEKLIY"
     gene            complement(33347..34288)
                     /locus_tag="L3M60_000399"
     CDS             complement(33347..34288)
                     /locus_tag="L3M60_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225103.1"
                     /translation="MRWILVFIFLYLIPLVVIFRNYKNFKRSCIYSSIYVVLATTIMI
                     SNIYMSGLNKIKESMYYHNYISDETYDEKYVSNFKEKESTDLKQDKKKNEEISNNKNL
                     NNKQNKNIQNKESNEEGSNNIKEKNNSDSNVSVNNKSEKQEDTTKENNKYTSKEKNSE
                     DIKKIDMESIGIFKKEIYNIERAALVPMRECIPYTKDIAKNITKLSSIKKDVTNARNK
                     CKEVVKEYENMQIPELSKEKYTETLSNARDDVKKAYELREKSMESALKLINTKSPKYI
                     GKITEYLDLSDKQIESFKNRLNDLKEEINNTETNSIN"
     gene            complement(34375..35067)
                     /locus_tag="L3M60_000400"
     CDS             complement(34375..35067)
                     /locus_tag="L3M60_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBN6225104.1"
                     /translation="MSLYAIGDLHFSTSVNKPMNIFGSNWDGHEKKIIDNWKEVVKEE
                     DMVLVLGDTSWGINLSEAKKDLDIISKLPGQKILIKGNHDYWWTTVTSLNKLYENMRF
                     IQTNFYEYKDYAICGGRGWICPNDVKFDETDEKVYKREEHRLRLSLESARKSGHSKII
                     VITHYPPTNDKLEESLFTKLFEEYNVEKVIYGHLHGKESFKMGLKGIRNGVEYTLASC
                     DYTEFNLIKVHD"
     gene            complement(35176..35988)
                     /locus_tag="L3M60_000401"
     CDS             complement(35176..35988)
                     /locus_tag="L3M60_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HBN6225105.1"
                     /translation="MEYEKWNEILAPYEHAVEELKIKFKNIRKEYLAKGEYSPIEFVT
                     GRTKKISSIISKLKRINAKDIETEIDDIAGIRIMCQFVEDIYAIVDLIKVRNDMTIIG
                     EKDYITNYKDSGYRSYHVIIKYPINSIAGSKEIICEIQIRTLAMNFWATIEHSLKYKY
                     DHYIPETLAERLRRASDAAFLLDQEMSEIREDIMKAQAMYQMKSIAIRDTLNRIQELY
                     DLGDTHKAMQYQRKLDRTETDKNITEILELKQEIDILLEQYKDTKVDDKVVQ"
     gene            complement(36039..36950)
                     /locus_tag="L3M60_000402"
     CDS             complement(36039..36950)
                     /locus_tag="L3M60_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6225106.1"
                     /translation="MNLTKVKGNTFFIKGGTNTGIYIFEDNTALMIDPGLCGSRPRRV
                     NKVLDENNIQVRYIINTHGHDDHYGACNQFKEYYKDVRIMSSEYDKLYIENPELFSKY
                     IVGGKSNVFMDNILKNKMLDDIKIDKSIKAGKIELNNEIFDIIEFQGHTPGSIAVLTK
                     DNVIFVGDLLIGDEILSKYDFLFLYDIQEQINSLEKLKNIDFEYLVLGHGKQVISKED
                     SYKLIEKHLNALKKYLHQIRELLVEPKSLEILLKNIINNNNLSNNYKEYHFFKSSLVS
                     VISYLVDLEEIGYILENGELLYYTKTK"
     gene            complement(37009..37740)
                     /locus_tag="L3M60_000403"
     CDS             complement(37009..37740)
                     /locus_tag="L3M60_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBN6225107.1"
                     /translation="MTSIKNKDILNCIDYSIKNKLFEKLNDVYKSLPVGNCLGCGNCC
                     MESVGINLIEFLNIFCYLEDRVDLKKSCISKILDYYFEEYTKKNPCPFKDNNNRCLIY
                     EVRPLNCRMFGHWKKEDYNKNLDNVIEKNNNYRELMKKQYGFEINDEVVNYRIDYCDR
                     FIPNKDYLSKSKRLSFFDELMTLDSNIYSSGCIDIDFRDRGIVEYFIESLLYRNLAYN
                     VKIRISKEPEIRKRTIDRIKRIILV"
     gene            37829..38080
                     /locus_tag="L3M60_000404"
     CDS             37829..38080
                     /locus_tag="L3M60_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225108.1"
                     /translation="MNRYTKIINMMGSYYTKDFEKEKKNVIKVREVKEDTVRKFFLQG
                     DCEVLVVFEDTGKEILIDDFSPEEDIKKYLGAKFINKKR"
     gene            38314..38733
                     /locus_tag="L3M60_000405"
     CDS             38314..38733
                     /locus_tag="L3M60_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225109.1"
                     /translation="MQIYFRVKAIGKRKPMLKLAPFDVSDNINCLKEAISEIVSKNVL
                     EYNEKIPEKSIVNFLTNEEIESQANIGRVGFGSIYNENKQSVEKAIEVALQAFEDGIY
                     KVLINEDVIEKLDSPINLKSGDIFTFIRLTLLAGRMF"
     gene            38758..43815
                     /locus_tag="L3M60_000406"
     CDS             38758..43815
                     /locus_tag="L3M60_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HBN6225110.1"
                     /translation="MLERTRVEKNPKIIENLKNNFTKSSSKQYYDLAKSLWDCIENSS
                     YYYREPEDKFLNMIKKYYNCHYYEIKNMDELLSGPLKNALELIFTSEQVEELCQLIKR
                     KMDYTYSGGYYRKGYRSNKLVDYFRGIIRFISSYVDLIYYDFSVIDYLTLPNKVLYYD
                     SIMSDKIALEIDKGNQEVISALEEVIFGENQTALLTRQMIIGIMKSHSDKCYEMISKL
                     LLAAKQQEGLRQQIVESLDEGTISASIYMLKTILDNNLERFSSVIRALDTWTGLGFSD
                     QKPGIIRKCLELSYKSLTDEKFRIESINSNDNLEVYFALWATATYNIYDSYSLIENLL
                     SCQEKYKKLVALYFLSHTESTYFKHSIASKNIKGDDLEILAWVITNFYVEGSVGYYYS
                     WQNRETSFDLSNTPIDKDFNARKEQFESLKHTLENLNSKTKSFSGSVFPWTWIEISSH
                     EILKCMMTLTAYDMNAQFIDVLMENCHLMSSDLRASLIVNFVNEPKTDRQREFLLECL
                     SDKSVSNREIVLTLLRKLELTDMELDKITNLLSLKTSSIRQTVIKILLEQDDSKLENI
                     ASSLIKSSNSQKCLGGLEIVNLLKDNTDRKELYKKLILLIKDIDKSKKNTSQVQILID
                     NLLGKAKNDYCKENGFGIYNPENKVQIPEQFDFKDNSPSLSKLDKIISNTSLSTNEDS
                     VSVATKKEAKKILDTDINKIIKVYEKFDELITENADYEYEVERWSGNVDKVLLGYSTY
                     NLTPIKYKVSNDTLDNYPLAELWRETSKKLNLDYKLILDILFYSISTCGYNEIEKSWF
                     SSIYSNVLDSKKQNKISTKLEKLKFNGLTRKLLRVLLNEFDHKEIFNYCLEVYYKLRN
                     LLTIEQCKKQRVEFTKNYYRQNEYCPALNAPILNYWLDKAHSFIYDDISFTKFFYTTF
                     SNYQLLEYKFPFYTSINVFARAHELGLMTKEQVNREMISGYNATNIIRTLTNPRWAGD
                     TLEKYPWLKDNLKTVTNRIIEIESRRGDMPTEVSHLANNIQYFEGINYFVNILVALGK
                     ETFQRGYSYGSNYSKKDVLSSLLKNCYPTKEDDSKKLKSLLKNTDITELRLIESAMYA
                     PQWVNIIEKYLNWKGLKSTIWYFHAHINEGFSAQKESEVALFSPISPQSFMDGAFDVD
                     WFLDSYKTIGEERFNILYKSAKYITSGGNSHRRAQLYADATLGKLDKDMLMKEIETNR
                     NQDKLRSFSLLPMKKGDLKEATFRYEFIQKFAKESKQFGAQRRESESKACAIAMENLA
                     RSAGFEDINRMMWALETEKLNEVAYIFKPKNIDRTSLWIDVDEKGKTSIKVQKGDKAL
                     KSVPKAIAKHPYVLELKSVQKDLKSQYSRAKQSLEQAMKNEIVFTVKEIANIYKNPVI
                     CPLIKNLVWVCDDNIGYVYNDKSKFFICNIEGEIYKLTAKNKLKLAHPVHLFESGKWS
                     EFQHDLFSKQIVQPFKQVFREYYRINSDELSEKTISRRYAGHQVQPKKTVALLRNQLW
                     TVDYEEGLQKVFYKENIVAKMFALADWFSPADIEPPTIETVEFFDRKTYKSLELASIP
                     PIIFSEVMRDIDLVVSVAHAGGVDPETSHSTVEMRIAIAKELLLLLKVNNVNFEGSHA
                     LIEGTLGEYSVHMGSGIVHKKGIGALNILPVHSQSRGKIFLPFADEDPKTAEIMSKIL
                     LLAEDSKIKDPSILSNL"
     gene            44130..46697
                     /locus_tag="L3M60_000407"
     CDS             44130..46697
                     /locus_tag="L3M60_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6225111.1"
                     /translation="MNLRFTRYIKSYKARSLAIAVSIILATSLVVGIGTLSRSAQQAD
                     VDRLKRELGTDHVYFKNIKKNQLKSIKSSKDIKNLGITSYYTSTDVNEKLPINILYAN
                     KNYLTSHSKLIKGRFPKASNEVVVEKWILNSMGLKPEINQNLTFKLYQKENPETFKVV
                     GILEDRFIEKQKGQCEIFSNLNESKLNDFTTYIEFNEGSDINTNIDNIIKNAMLDEKS
                     IGRNSMLIESTMKNGTLDNASKYVAIAMSLFAGIVIYSIYVISIYQRVQEYGVLRAIG
                     STNFRIFKLMLYELFILALIAMPIGICIGMGGAQIFNRSVGNIQFEGNINVTPFVIPD
                     KIILLSIACTILIILIISFFTYLKIRRISPIDAIRKTFGADKNVTTVNYLISKLTLNM
                     SVTKSISAKNIFRNKKGFIIIVLSMSIGGIIVIKDNYKYSFLDVQNKSTQEQTYMNAD
                     FILSNVYLNEIGSNTSFKDIKGFSDSQIDKIKNIDGIDKVKTASILNTRIEVNKINRL
                     DYYEAINSNPYYKLNPLFIKNKTTGKYTLKQKLKGYNDEMIKSLEKYLVSGNINLKEM
                     KKENLAIVYVPEISKTQKYTKTFTPGEGTPAIDIKVGDTIKVKYPKGGMDTGLYESLK
                     DNYEYLEYEFRVGAIISYTFADNGMYSGDDGVDVITSSEYLKKLTGVDKYNLVYADLN
                     ENANKKKINNLLGKIGSEVSGTTTVDMMVEKENSDKMTSRTMIYSYGIVAIMFIISVL
                     NIINNISYNLTSRTSEFGMLRAIGISERGFKNMILYEGILYGVLSSIITIVSGLIIQF
                     KMYYTQGFVSYGLGFSIDYKIYILVVVANIIVGILATYIPSRKINKISIVEAINITE"
     gene            46709..47380
                     /locus_tag="L3M60_000408"
     CDS             46709..47380
                     /locus_tag="L3M60_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225112.1"
                     /translation="MPILETINLGKIYGKKETSVHALKNANLKINKGEFVAIIGPSGS
                     GKSTFLHLVGGLERPSNGTIKVAGKDICCLSDKELARYRRQKVGFVFQQYNLIPVLNV
                     KENIELPLKLDNKKIDKEYIEDLINLLGLNERKNHLPNQLSGGQQQRVAIARALSAKP
                     SIILADEPTGNLDSKTTEEVMDLLKSSIKKYNQTLIIITHNENIARKVDKIISIIDGE
                     LKLTL"
     gene            47407..47748
                     /locus_tag="L3M60_000409"
     CDS             47407..47748
                     /locus_tag="L3M60_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225113.1"
                     /translation="MLLILGNLNLNLDFDYRIIREENDDVDIFIDINYRSLDIDTDGS
                     NLFNSRIQFPFVRALILRLNKNNQCMTIHLLRDIDLFSAFANFEVDYTDSIINIKNQN
                     EKVILNKSIKK"
     gene            complement(48101..50743)
                     /gene="adhE"
                     /locus_tag="L3M60_000410"
                     /gene_synonym="adhC"
     CDS             complement(48101..50743)
                     /gene="adhE"
                     /locus_tag="L3M60_000410"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBN6225114.1"
                     /translation="MEKKENVVEKSSVEVCSPEIVNSVETLRMRLEEIRLAQKEFATF
                     TQEQVDKIFLAASTAANQQRIPLAKMAVEETGMGIVEDKVIKNHFASEYIYNAYKDTK
                     TCGVIEKDEAFGFTRIAEPVGVLSAVIPTTNPTSTAIFKSLIALKTRNGIIFSPHPRA
                     KNCTIEAARVVHDAAVKAGAPKGLIGWVDVPSIELTNVVMAEADLILATGGPGMVKSA
                     YSSGKPAVGVGPGNVPAIIDESADIKMAVSSILVSKSFDNGMICASEQAVIVPEKIYE
                     EVKKEFKYRGAHFLNKEETEKVGKVVIIDGSLNAKIVGQPAHIIAKMADVEVPKTARI
                     IIGEVESVELNEPFAHEKLSPVLAMYKSKSFEDAVAKAEKLVADGGYGHTSSLYADSI
                     NHPERVEKFVNAMKTCRVLVNTPSSQGGIGDLYNFKLAPSLTLGCGSWGGNSVSENVG
                     VKHLLNIKTVAERRENMLWFRAPEKVYFKKGCLGVAAREFKDVMDKKKAFIVTDSFLY
                     NNGYTKKLTDLLDEMGIKHTTFFDVAPDPTLACAREGAKAMADFQPDLIIAVGGGSAM
                     DAGKIMWVMYEHPEVDFQDLAMRFMDIRKRVYVFPKMGEKAYFAAIPTSAGTGSEVTP
                     FAVITDQDSGVKYPLADYELMPNMAIIDADMMMEMPPRLTASSGVDALTHALEAYVSM
                     LRTEPADGLALQAGKIIFEYLPRAYKNGKNDKEAREKMAMASTMAGMSFANAFLGICH
                     SLAHKLGAFHHVQHGVANALLINEVIKFNCAEAPNKMGAFSQYRYPDCIQRYAEFASF
                     AGIKGNTDQEKVDNLIKAIDELKAKVGLPKTIKEAGVEESKFLEGLDAMVEQAFDDQC
                     TGANPRYPLMSELKEIYLKVYYGK"
BASE COUNT        17281 a         8514 c         6206 g        19265 t
ORIGIN      
        1 aagacaattt tcaggtggat atcatgcaaa tagcaataaa aactgtattc taattttcaa
       61 tagtattttt tttatatcta tggttttggg aataatttta aatgattcta atcctattcc
      121 gttattttta gtgatattgt ctagttttaa ctttctaagc atttttattt ttgctccagt
      181 atgtcatgct aacaatccac taaatgaaac aaaatataaa aaaaataagt taatgtcaag
      241 aattatatct ataactttat tcttgattat tcttctagga tgcaaatact tatcttttta
      301 caattatgtt gtatacactt ttttagcttt actttggata aatttattac taattattca
      361 aatcattata aataaaaaaa taatataagg agatattaac atgaaaaaaa ttctatatca
      421 ttttatgaaa gcatgtggtg tattagctat gtttgtagca tttttatctg caaacacaac
      481 ttctctgtgg catgtatatc aaccaaaaac accagaaaaa ctaaaaaaat tctaaaaatc
      541 accaaaaaga aggggctgtc gcataagcaa attaaatttg ttctgtgaca gctctttttt
      601 tacttatttt aataggtata tttttaattg ttttatatta agtttatatt ctaagtaaaa
      661 ttatgcataa caaataaagg tataaaaata ccattttttt agataatatt taattataaa
      721 caataaattt agaatatttt ttttaaatga tgaagatata ttccatgttt attttttacc
      781 attttattgt gtagtctaga aatattatga gctatcgcta caataataca ttctgataga
      841 acattttctt tacctcagct taaaaatctt ctaaatttca tatttttttt acttgagcga
      901 aagctccttc tgcttatata cttctattca ttctaggttc aaacccttcc gggcttataa
      961 ttctctttaa gttttctttt cttttttcat aaaataactt agatacttgt aagtttttag
     1021 tcctattttc aagaggaatc ttactattac ttctcttaat acaactgctt ttatattgac
     1081 aataacagca ttcttcacaa gtataacaat ttatttcact tttatatcct gtttttgatt
     1141 ttctattgat tattttatta gcaaaagttt ttgccatttt tacaaatata ataatcattt
     1201 ttttcatcat aatccatatt atcaatacgc ccaatctcat ttttatattt tctagtttta
     1261 ctaatttcat aatttgcagg ctttatgaaa gacaattgat tattacgctc taaaaatgta
     1321 tagttttcct caccttcata gccagaatca gctactattt taggatattt aaactttata
     1381 tggtcttcaa tagattttaa aaatgatatt aatgttgtag tatcagtagg ttgatgccct
     1441 gaacttacca aaactatgta ttctgaatct acaccaaatt gtatgttata tgctgatttc
     1501 aattggccat ttttcatatg gtcttctttc attctcataa aagttgcgtc attatctgtt
     1561 ttagaataac tatttcgttc acccattata tgaaggtctt tagtatattt tttcaatcta
     1621 tcaatataat tatttaattg ctctatagat ttttgaatga tagatttttt ctttcctttt
     1681 tcatgaacaa atactatatt taattctttt gatatttttc ttagtttctt taaaagtttt
     1741 tttagatgat agattttgat tttatcttta tagattattt taataccaaa atcaatctca
     1801 catttcaata tgaagtcaga tctattagca gattactcta tttttgtacc atcaataagc
     1861 agagtatcct ttgaaatctc cttattatat ccaataaagt ttgtaaactg tgcaatcaaa
     1921 tttttgctta cttgtgaaaa ataaatacta cgaaatcttg caattgttga atggtctggg
     1981 gcggaggctc cgcctagaag atacataaag ttaatatctc ttttgcatgc tttttctatt
     2041 tctcttgaag aataaatgca gttcatgttt gcatatatta taattttcaa aagttgtcta
     2101 ggggttactt tatttcctct aattcgagaa taagtcttat ataatttttc taaattcatc
     2161 tcctcaataa tttgacttaa taaccttaca gaatcatctt ttggaatcat gtaatcaata
     2221 tttagtggta atttaaattg ataattatca ccataagtag tataatcaat ttgtatagta
     2281 ttttgttttt gcatacaatt attatacaag aatcccaagc tcttttgaac ttgggatttt
     2341 tttatacaaa aaaatggcgt agcactattt tctagtgcga cagccccaac tctatattat
     2401 ttagaaatta ttactgtaat tttactattt gaacatgtac attcaaaata caagtctaga
     2461 aattagcttt atttgtaaga taaataaaat cagtcccatt atcttctgta tgcaaactaa
     2521 ttaagttatt cttttttaaa tatttaattg catatttttc ctatatacat agcatttata
     2581 aagaaagttt tatatactta aactattatc ttatttaaag atattaacaa taaaaaccta
     2641 aaacaagaat tgtaatacaa gataaaatat ttgtctaaat aatattgctt atcactaaga
     2701 caaaaactaa attaatataa aaatagaggt gattagatat aatgaataat tataattcta
     2761 taaataactt acaaattgga aaaacatatt ctaatgacga aataaaagaa atatttaaat
     2821 gttctactca aggtggtatg ataaaatcaa atactacaaa gactctagtt atattctctg
     2881 atcatacaaa aagtatatat gatgatattt ggtatgataa tacgttaata tacactggta
     2941 tgagaaagaa gggtgatcag gtattaaaag gtaatcaaaa tataacactt tatgagagcg
     3001 aaactaatgg aataaaagtt catttatttg agacttttgt ctcaactaaa catatttata
     3061 aaggatttgt taaactgtca ggtacacctt ttataggtga gcaagaagat gttgatggaa
     3121 taaatagaaa agtatggtta tttcctttga aattaataaa taatatacct atagaaaaaa
     3181 atctattaga gtataataaa aatcttaaat ctaaaaaagc tagaaaaatg actcaattag
     3241 aactaaaaga taatataaaa aataaaacca tctacaagaa ctgttatatc aaaaccatat
     3301 gtaagagatg agctagtttc tcaatatgca aaaaatagag caaatgagat ttgtgatcta
     3361 tgtggttcta atgctccctt taatgctctc atggtaaccc atacttagaa tatcaccata
     3421 ttaagtggct ttctgatggt gacgaagata gtttatataa tactgttgca ctttgtccta
     3481 attgtcataa aaaaatgcat atatgtaatt taaatgaaga tatacataag ttattagaca
     3541 agataaaaaa ttacgaaaag taaattagtt aaacaaatat aataaattta aaaactagga
     3601 tctgaatatt tttctaattc agatatccta cctactgaat ctatttgaaa agcatagaaa
     3661 tttttcttat ttacaagaat tgctgaatct gaatcatcta ttttataagt aatcccaaag
     3721 aaataacatt cgtccaaata tgtttgtgca aaattaggta aagaatctgt attttctatt
     3781 aaatatgctt catatttatc gcctagaact ttattagctt tctttaaagc taaatcatag
     3841 ttactaactt ctgttccatc tttattgtaa taatgccaac tatctcctaa tttaatccac
     3901 ccatatttct ttattccatt tgatacatct atacattgtt gaattatact actattttct
     3961 atactaatgg cagttatttt ggaactaccc tctaatatgt tcttatcact atttgtatct
     4021 accaaaataa taggatcatt tttagctatt gtagaaccaa caagtgcata cactaaatct
     4081 gttccacttg ctatataaaa ttcttttgct ccattataga acttatttac tattttctta
     4141 ttagtatcgt atctatctat accacctaat ctagttgaat tagtatcatt tactaagtta
     4201 tcactcattg atgaagtacc acctattgca taactttcta ttccaggcta atattaaaga
     4261 tatacaacat agattagggc atgctaaaat atcaacaact atggatacat attcacatgt
     4321 aactaacaac atgagaaacc agacagtaaa tatttttgaa agcattgtag agtaaatttg
     4381 ccaccgaaaa ataattacgg tggcaaaccg gtggcaaacc cacctttata gcatcatata
     4441 gatagctgaa agcctcctat tctctaacaa gtttaactat agtctcgcaa tgaggcgtat
     4501 tcggaaacat atccatacac ttaaccatcc taatctcata accatatctc ttaaaatcaa
     4561 ccaaatccac aaccaaagac ttaggattgc aagaaatata aacaatctcc tttgcaccaa
     4621 atccacaaat atccctaata gcatctttat gaatacctgg tctaggaggg tcaacaataa
     4681 tcaaatcagg cttgtccttt aaatccttaa caaccttagc cacatcccca gcaataaact
     4741 cacaattagt aagcccattc aacttagcat tctcattagc agcaacaaca gcctcctcaa
     4801 ctatctcaat accatacacc ttcttagcag caccagccat aacctgtcca atagacccag
     4861 ttccactata caagtcaaat acaaccttat cattgtaatc tccaataaaa tccctagcaa
     4921 tactataaag aacttctgct cctttagtat tagtctggaa aaatgaaaat ggagaaatct
     4981 taaacttaag acctaaaatt tcttcttgta tataatctct tccatataaa actcttaact
     5041 catcacattg gaccgcatct gctaaaccat cattaatagt atgaagtacc cccaccaact
     5101 cagattttaa atcaatccct aaaagcatat ctttaaactc attcatatca aaatcttctt
     5161 gagaagaagt tactatatta accataatct cattagtatg aatccctttt ctaactacaa
     5221 gatgtctcaa atatccttta tgattcattg ttctatagta aggaagcttt ttttcattaa
     5281 aaaactctac actagaaact aatatctttg agaagtcttc atcaaccaac atacactcct
     5341 caacagtctg tatatctata tgttttcctt ttttatgaag ccctaatgtc aaaggtccat
     5401 tttttacttc atctccaaaa gtgtattcca tcttatttct ataatatttg ttttctggac
     5461 ttccttctat ccctaaaaat tgaaatccaa ataaatcttg ctttaaaaat aaatccataa
     5521 cttgtttttc ttttatttcc aattgttttt catatggcac agacagtaat gtgcatcctc
     5581 cacactctct aaagtgcttg caagcttctt cagtttcaag tggtgagttt tctaatagtt
     5641 ctatcatctt aacttcagct ttcttacttc taactttttt aactccagct ctcacctttt
     5701 gaccacttat tccacccttc atatggataa ctctatctcc aacttggctt aaagatgtac
     5761 caccaaattc catcttatct acttcaaatt ctataacatc ttttttcttc acagacattc
     5821 ctccagttta ctatagtatc tttattatct ttcttcaatc aattttattt cttcatctgt
     5881 taactctcta tattcaccta actctaaact ttcatccaat tttaaattac ccatagacaa
     5941 tctttttaag tatactactt ttttgcctac actttcaaac attcttttta cttggtgaaa
     6001 cttaccttca tgtattgtaa gctctatctc tgattcatca tcactcttta agatatttag
     6061 ctgagatggc atagttttat atccatcatc aagtactaca ccttctaaaa aagcttctac
     6121 atcttcttca gtaactactc catcaatttt tgcataataa gtcttaggca catgcttctt
     6181 aggagataaa actctatgag aaagctttcc atcattagtt aaaactaata atccctctgt
     6241 gtctttatct aatcttccaa caggaaatgg ttcaaaagct aagtatgata aatcaattaa
     6301 atctaagacc gttgggtcat atttatctgt agttgccgat acatatccat ctggtttatt
     6361 catcattaaa tatatatact ctctatatat aacttcttct ccattaaaca atatctcatc
     6421 attttcagtg tctacttgag ttcctggatt tgaaacttca cttccattta ctactacaga
     6481 cccttgctta caatacttct ttatttcact tctacttcca tagcctaaat ttgaaagaat
     6541 tttatctata cgttgctttt tagccattaa aaatgctctc cattctcata atatttttct
     6601 ttttcaaata ctgtaaatgc taaatccata ctttctacat ctaaattagt tcttatacta
     6661 tcagttatta ttcttgctac tgtatcttgt acttcttgtc ccctgtcaaa ccaagctatc
     6721 tcaacaaaag gatatacatc tgctatttta ccatctctta ttgcaactga ctttatacat
     6781 tctatttcaa agtagtctct tggacatttt acagcttcaa ctaaatcatc tatcattttt
     6841 tcacttattt tacatatatc atcctcatta attcctctta tttttatctg tggcatagtt
     6901 tacctccttt attttaaatt tttagtgaat tatcatccac ttaattacct tcgataacgt
     6961 ctgaagtggt agcttcttgg tcaagataac tattgttatc agtatctcca aactaataag
     7021 gtagttccta cccttatagt tagcattata tagctaagtt tcctgaattg tttatattct
     7081 ttattatttt atttaatcct attgtcttct atttaacatt atacttgtaa tttatagtta
     7141 ttacaattta ctatatactt tttttgattt aacgttataa tataagtgat gggtaaattt
     7201 taaaagttaa taggaggagt ttaaaatggc attaattact acaaaagaga tgtttaaaaa
     7261 agcttacgaa ggtggatttg ctataggtgc atttaacata agtgacttag aacaattaca
     7321 aggtgtttta aaagctgcta aagctaaaaa ttcttatgtt atgatacaag cttctatgtc
     7381 agctgttaaa tatgctggac ctcatacatt agttgaaatg gttaaggctg cttctgatga
     7441 aataggtgta gacgtagctc ttcacttaga ccatggtcca aatatggatg ctattaaaac
     7501 ttgtattgat gctggtttct cttcagttat gatagatggt tctcattttg actttgaaga
     7561 aaatgttaga ataactaaag aagctgttga atatgcacac tctaaaggtg ttgttgttga
     7621 agcagaactt ggtgttctag ctggaacaga agatgatgtt acttctgatg ttcacaagta
     7681 tactcaacca gctgaggcag ttgagttcgt tgaaagaaca ggtgttgatt cattagcaat
     7741 agctattgga acttctcatg gtgctttcaa attcaaagga gaagctaaat taagattcga
     7801 tatactagaa gaaatccaaa gcaaattacc aggtttccca atagttcttc atggtgcatc
     7861 tgctgttgac caaaacgctg ttgctacatg taatgaattt ggtggaaata tagctggtgc
     7921 taaaggtgta cctgtagata tgttaagaaa agcatcttct atggctgttt gtaaaataaa
     7981 catggatact gacttaagac tagctatgac tgctgctatc agaaaattct tagctgaaaa
     8041 tccaaaagaa tttgacccaa gaaaatacat tggtgctggt agagatgcta tacaagctgt
     8101 agttgaaagc aagatagatg atgttttagg ttctgctaac tctataaact aatataactt
     8161 ttaaatattt ataaaagaaa agagatgact taaagtaatt aaatttattt ttaagccatc
     8221 tcttttaagt tgtatatatc tttttatata aacaaatata tgttattttt ttactgtatt
     8281 tttaactatg gtaccatctc taaacccttt agctatttct tccacattca tttttacatt
     8341 attagaagta tctgcaaatg atttatttgt aagcaaacaa aatccaccta aaccaatcag
     8401 taccgttaat cataaagcca taaactattt ttttatgatt attatatgct tttttgttat
     8461 tttctaaata ataccataaa aatgtttatg tgctaataca aaaacacttt tagcgataat
     8521 actccttact gtgattggta agttataaat gtgttattca atctagtaga ttcttacttt
     8581 tttagaagac tggaacaaat catatgagta cacatctata gtgtttctac caaaaaacaa
     8641 aaaaatatca ctggaatcaa aacctccagc aatatttaat aataaaaaat atatttacat
     8701 aaatctatgt acattttcct aataaagtat atttcatcaa ttctaattac tttaccaaac
     8761 taacaactat acatgaatat gtccactctt tatctttcta tggatggtag aaggatatat
     8821 acctataact tcagcagcct ttgttattga accatactta tttatagcct tacttatcat
     8881 tatttggtcc actattttat atgcatcatt aagattcata attccattta ccactattgg
     8941 tgataaatca tctgtttcat tatcaatcat atctaaattt ataagcatat taaattcatc
     9001 ttctccaatt atatttttag ctgataatac tacgaatctt tcaataatgt tttttaactc
     9061 tcttatgttt cctggccaag aatattcttc tagtaactca ataaccttag gtgaaacttc
     9121 tacttctcta ttatactttt cattgtaaag ttttaagaaa taatttacta gtggtactat
     9181 atcttctttt ctttctctaa gtggtggtat ttttactgga ataacactca atctatagta
     9241 caaatcttgt ctaaatttaa gattatttct aaggtcttca tgagaaatat tagtagcact
     9301 tatatatcta acatctatct ttataggctt actaccacct attctagtaa tagtattttc
     9361 ttgtataact ctaagtaatt ttttttgcat ctgcattggt aattcaccta tctcatccaa
     9421 gaaaatagtt cctccatttg cttcttcaaa aagtcccttt ttgccttttt tcttagcacc
     9481 agtaaaagaa ccttcttcat atccaaaaaa ctctgactct aataattcat ttggtatagc
     9541 tccacagttt atagctataa aaggtttgtc tcttcttgaa ctatacttat atattgttct
     9601 tgctattatt tcttttccta cacctgattc tccatttata aatatagaag aatctgatgc
     9661 tgcagctctc ttaacaactt tttttatatt gtccataatc ttactttgac ttatatattc
     9721 tgtgtcttct gatatatcac tatattttat ctgagattca agcttgataa cccttgaaat
     9781 atctcttcct gttagtactc ctcccttaag ctttccttta gaatcccatc ttggaactgc
     9841 tgtgtatgca ataagcttcc catctgggta aacgatattt ctataaatct ttttcttcac
     9901 tttaagcatg tttaatatta ttggttcttc aactaatcct tgtcttacaa ggtctgttat
     9961 atttctacca agtacctcag ttcttttgaa gggtaatatc tcgtcactga cagtattcat
    10021 cttttcaatt accccatctt tgttaaacac aacaacaact tcatctatat gctctattat
    10081 atcttcaatt actttatcag atattagaga cttctcaaat aaataaacta cattattttt
    10141 attattatta tctttcatat tgtatatatg tgcagtcatc cttaactctc tgaaagctat
    10201 tttcttttta ctatctgtca gtatttctgt tttgtcaaaa tttgaatcta attcatttat
    10261 atttttaggc ttttttatat ctagaaaact aaaaatacta tctattagat gattacagta
    10321 tattatttcg cctgattctt tacataaaat ggctggattt tctacatgtt ctataagccc
    10381 tattaaatta ttatccatta atattcgcac ctttcattaa gtaatttatt gaactacata
    10441 caaaatttat tgtttgctta caataaatac catcagcaca tataatcaac tccttataat
    10501 atttgtcttt tcaagtagtt tctttcatta aaacgtaacc aagatataat tttaatgatt
    10561 atagtttgtt aaaaatttca ttttgctttt aattgtattt tatcacatat ttagtaatta
    10621 tttgtctatt taatacaatt taattaaatt taagatttat tgaaaaaaca aagataatcc
    10681 ttacagaata tgactcacaa tttgtaagca atatacatat aaggattatc tagattatct
    10741 tgtattataa aattttattc aaacagactt tttaataact gacaaatacg cactagcaat
    10801 aacttatcaa agggttaata attatgttac taactaataa gacttattca atcagtaata
    10861 tttatatagt ttgtatctat taagctttta tagcttctaa taaatcatca acgctatcta
    10921 atttttctat aagcattgga actatttcat gtaagtctcc aactactcca tagtcagcga
    10981 tttctaaaat tggagctgct gggtttttgt ttatagcaat tatgaaatct gatgattgca
    11041 tacctgctaa atgttgtatt gctcctgata taccacaagc tatatatagg tttggtttaa
    11101 cagtagttcc tgtttgacct acttggtgag aatggtctat ccatccagca tcaacagcag
    11161 cacgagaaga accaactact ccacctaatt tgtcagctaa tttcttaagc atttcaaatc
    11221 cttctggtcc acctaatcct agaccacctg atactataac atttgcatct gtaagagata
    11281 ctaaatcttt tttagtttta actgtttcta aaacagtagt tcttatatca tcttgagtta
    11341 ttttgtaatc taatgctata acttcaccag ttcttgtttc gtctttttca gctttatcca
    11401 taacaccagg tctaactgta gacatttgag gtctatggtt tggacaaatg attgtagcca
    11461 ttatgtttcc accaaatgct ggacgagttt gttttatctt cttatcttct gggtctattt
    11521 ctaatttagt acagtcagct gttaatccag ttccaactct tgaagctatt ctaggtgcta
    11581 agtctctacc tatatgagtt gctccgaaaa gcattatttc tggttttatt tcatctattg
    11641 catcttttat tacttttgta tatgcatcag ttgtatattt ttctaaaaga gcgtcatctg
    11701 caacataaac tttgtcagct ccaaaagtga ttaattcttt agctaatcca tcaacatctt
    11761 ttcctaataa tattgcacaa agctctgcat ctacttcttt agctaatttt cttccttctc
    11821 ctaataattc gataactact ggagctattt ttccttctct ttgttctgca aatatccata
    11881 cgtttttata agaacttaaa tcttttatat cattcatagt ttgtactcct ccaacttata
    11941 taatgtgctt ttgttttaga ccgattatta ttttactagc tatttcatca gctttacctt
    12001 ctaagatttc tccttttcct cttggttcag gagtaaatga tctaaatact ttagttggtg
    12061 atgcttttaa accaacttct tctttattta catctaaatc atcaattgtc caaactttta
    12121 catcatgttt atatgctttt actattttat caacagacat gtatctaggt gtatttaact
    12181 cttttactgc tgttaaaagt acaggtgtac ttacttttat taattcatat ccatcttcta
    12241 attgtctttg aactattata tcttttcctt ctattttaaa atcttgtaca tatgtaactt
    12301 gaggaatgtc taatttctct gcaatttgtg gtccaacttg tgcagtatct ccatctattg
    12361 cttgtcttcc tgcaaatatt atgtcatagt cacctatttt agatattcct gctgcaatag
    12421 tatttgatgt agcccaagta tcagctcctc caaatgctct atcacttaat aatattgcat
    12481 catcagcacc catagctaaa cactctctta gcataaagtc tgcttgagga ggtcccattg
    12541 ttataactgt tatagttaca tcatcatact catctttaat ttttaaagct tcctctaatg
    12601 catttgcatc atctgggttt aatatacttg gaactccatc tcttataaga gtacctgttt
    12661 ctttatttat tcttacttcg ttagtatctg gaacttgttt tacgcaaact aatattttca
    12721 aagtctatac ctccaatttc attagattat ttattatttt aatacgtttg atgaaattac
    12781 cattttttga acttctgatg ttccttcgta tattgatacg attctagcat ctctgtacat
    12841 tctttcgatt tcataatctt ttataaatcc atatccaccg tgaagttgta atgctttata
    12901 agcaacttca tttgcaattt ctgatgcata gtatttagcc atagcagatt cttgagcaac
    12961 tctcattcct gcatctctct tttgtgctgc atcataaact aatcctctag ctgcacaaac
    13021 ttttgtttcc atgtcagcta tagtaaattg agtattttgg aatttagcta taggcttacc
    13081 aaattgaact ctttgttttg tatatttaac agcttcatct aaagcacctt gagctattcc
    13141 taaagctaag gcagcaacac ctattctacc aacttctaga gtacccattg ctatcttaaa
    13201 tccttgacct tcttttccaa gtaagttttc ttttggaact ttaacgtttt caaatattaa
    13261 gtcagaagtt tcagttcctc ttatacccat cttatcttca tgagctcctg ttgagaaacc
    13321 atcccattta ctttctacta taaacgctga tgttcctcta agtcctttac ttctatctgt
    13381 aactgctatt actatagcta catcacagaa tggtccatta gttatgaaag tttttctacc
    13441 atttaatata tagtggtctc caactaattc tgctgttgtt tgttgtcctg cagcatcaga
    13501 accagctcct ggctcagtta aaccaaatgc tcctattaat tcacctgttg ctataccttt
    13561 taagtatttt tgtttttgct cttcagtacc attgtaaagt attggtaatg aagcaagaga
    13621 agaaccagct gtaacaaaag tagctgttga agcacatctt ctagctattt cttccattac
    13681 tatagcgtaa gaaacatatc ctgctccccc accaccatac tcttctggga tattactgct
    13741 tataaatttt gcatcagcta attttttgat taattcctta ggcattacac ctgttttatc
    13801 catttctgct ggtaaagtat ctaattcttt actaacaaaa tctcttactg cttttcttaa
    13861 aagttcttgt tctttgttat ataacatagc ttcctccaat tttcatattt tatagtaaat
    13921 ttattttaat gaacttacgc taaactcatc atctcagcaa aagtttgaat acgagttctg
    13981 gcttgttcat tattctgagt ttgttggtcg atttcaacat ataaagtagg tattccacta
    14041 tcttctatat cttttctaac taaaggataa tcgtattctt ctgggtcaca gaatttcatc
    14101 atacagaaga taacaccatc tatatctttc tttttaactt catctactat aagtgaccca
    14161 cgttttttct tagggtcata agctaatgaa catccttcta tgtttgacca ttgtcttgct
    14221 aatctttcca acgcatcatc acctgctggt acatctgttc tgaattgtct tgtttcttgt
    14281 gctaagtcat cagctacaac tgatatattg ttgtcttcta aaatatctaa tatatcttta
    14341 gaatcagcta atatacctgt taataaaact ttctttccag aacagacttc ttctggcata
    14401 gcatttaatt ttgctattaa atctttcact agtttagtgt gttcttcttt tctcataaag
    14461 aatccactct taattactaa gcttcttatt gatggtttta tagtattaga atgtttataa
    14521 gctacttcaa caaagtctct catagttttt ctatgttcat tgtaaacttc tatactttca
    14581 tgaatttttg cttcttctat ttcatatcca catatttctt caagctctct ttttacgcct
    14641 ttgtactcac tgattaagta ttttacacca gcttcaagtt ttctattttg tgggtgtact
    14701 aatgatatat attttatatg aggtactgct gatttccagt tttgtcctaa acaaattaat
    14761 gtatcacaca tacctggtat aataactcca gataattcat cataagcacc ttttaatcca
    14821 tattctaaac atgattgcat tattgaacat gcaaatgcag ggaaatattg tttagctaaa
    14881 tctaattctg tttgtcctcc ccatatacca actggaagca ttccagctgc atgtataatt
    14941 tcttctgggc aataaactgg gaaacaacct atagcttttt taccagtttc acttttatat
    15001 ttttttacag ccgcatttgg attttcaact acttctttca ttttagataa aatagcttcc
    15061 attatatctc acctctatta agtttttttc tttcttccat tacttcaact aaaccttgaa
    15121 ttcttgtttc aaattgagca ttagtgaaag ctctagggtc tgcttggtca ccatcaaatc
    15181 cagcatatgg taatccagtt tcttcagctg ctcttctttg catttcatat tgtattaaac
    15241 tcataagttt acaacttctg ttcatatgat aaaatgctcc atcacattta ccctctatta
    15301 aagaatcaac tctatatttt gtcatacggt ctagatttac attgttaaac atagtactat
    15361 atgctactgc cattccatct aaatcattaa cttcatattg taatgcccaa gcatgtgggt
    15421 aaacactacc tgtcatgtta actccaaatt tagctaatgt tttcatcttg tatcctatat
    15481 atggccaaca aggtatacct tccatcatta ttctgtattt ttcttctcct ctgaaagaag
    15541 atttaccagt tttcatgttg tcctctaatt cttctataag taacttaaat gcttctgtag
    15601 tctctttttt acctctagca caaactatta cagccatata agtaaataag tcaaatccat
    15661 tcattggaga aggagaagaa tctgctggta aactcataga atacttccat agtcttccgt
    15721 tttcagctga tattttcatt acctcttcaa atttcttagg gtcaaatttc tttcctgata
    15781 taatttctag ttgttttata gcttcttcaa attgagcttt aatataatct attcttgatt
    15841 gagtaacttc atcttcatta ttgaaagttg tatcaatcat tattaaaggt atatctaatt
    15901 ctcttgaaat attttcatac cattttataa cttggttaca tatattgtta cagcaaagta
    15961 ggaaatctgg agctggcata tccaaagctt cacatccacc attttctaaa agaccaaaat
    16021 ttgttcttgc atatgcacat aggtcaatag aatatccttt agattcagcc atttcacata
    16081 attctaaaga acctttttta gctgctactc cagccgcttg gttttctgga tataatacgt
    16141 ttaagtcaaa tacttctgct aattcttgag gaaatactga tgttgaccaa cctacaggtc
    16201 ttccttcttc tttagcttta aatgcatttg catattgttc agctaataaa tcattaatta
    16261 ctactctagc ttcttttttt tcagacattt tcatccctct ttttatatat ttttcacttc
    16321 tttttgtgat tcttttgctt catcaaaagc atataacgct gctcctaaag caccagttaa
    16381 ttgaggtata tcaggtacaa taatttctgt gttgatttct cttgccatag ctcttacaat
    16441 accactattt ctagcaactc caccaaccat aactacattt ctttgtactc ctattctttt
    16501 tactaggcta gaaactctct ttgctactga agtatgaata cctgctacta tatcttcaat
    16561 ttttgcattt tcagataaat gtgatataac ctcagactct gcaaatactg tacatgtact
    16621 gcttattgat acttcatttt gagaattcat agatatactt ccgagttcag atacatcaac
    16681 ctctattatt tttgccatta catctaaaaa tcttcctgta cctgcagcac atttgtcatt
    16741 cataagaaag tttagtagtc ttccattatt atctaacttt aatacctttg catcttgacc
    16801 acctatgtca ataatggttc ttgtctctgg aattatgaaa tttactcccc tagcatgaca
    16861 gcttaattca cttatttgct tatcagcatc tgaatagttc attcttccat atcctgtaac
    16921 tacaactttt ttaatatctt ctcttgcaag acctgtcttg ccatataatt tttctaaaac
    16981 tcttgatggt ccagtagtcc cagtaccaga ggatattgtt tcagaagcta caatatcttc
    17041 tccattcttt aagattactc cctttgatgc agttgaacct atatctaatc ccattgtgta
    17101 catctttagc attctccttt taaaaaatct agaattacta attaatatct tacaacttta
    17161 ctatctttaa agttatttat tttctcttca tcatagccta aagattttaa tacttctaca
    17221 gtatgttgac ctacttttgg tgctggtgta tattctttaa ttccttcatt tctaaacata
    17281 actggagtat taactaagac acctgtattt ccgctatcat atgttttctt aaataagaag
    17341 tcatttgccc aagcttgttc gtcatctaat aaatcttcac agctttgaat tttttcaaat
    17401 ggtaaatctg cttcttctaa taaagctgac cactcgtcta gtgttttttc taacatagct
    17461 tctccaacta ttttaactaa atcttcaaca tgattaacca ttgaatctat gttattgtat
    17521 ctatcatctt ctaatatata ttctctattt ataaccttac agaatttgcc taaccacttg
    17581 ttgtattgta ttaaagctaa ttgaatccat cttccatctt tacatttata tgtagtcatt
    17641 aatgggctgt ttggattttc tcttgataaa ggcatttcat ttccgtattg tgctgttgtt
    17701 atcattgttc ccattccata tatagctgta tggaaaagac ttactgttac tctctcacct
    17761 ttaccagttt gacctttttt atgtaatgct gctaaacttc ctgccgctag tgctagacct
    17821 gcataatggt caccaaatcc tgctgctgta tttgctggag atgttccttt ttccataaca
    17881 gattggctaa ctcctcctct tgcgaagtat gcagtatagt caaatcctgg tttatctttt
    17941 aaaggtcctt tttcaccata tcctaatatt tgagagaata ttaatcctgg atacttatct
    18001 tttatttggt cataagctat acccattttt tctaatgctt gaactctaac attagttaca
    18061 aatatgtctg cttctgataa taatttatgt aatatttcta ctccttcttt tgattttaca
    18121 ttaatactta cacccttttt atttccattt tctaattcaa acatagggtt ttcatcatct
    18181 gatgctggag atttaaatgt tccacccata actcttattc catcaccttc tataggttca
    18241 atcttaataa cctctgcacc ccagtcacct aacatttttg cacaacatgg tgctgcaatg
    18301 aaacttgaaa gttctactac tttaactcct tctaaaagca taacgttttc ctccttaata
    18361 gtatcaatta taaaatttca attaaatcac tcataaaata ctttttctga tattaactgt
    18421 ttgatgtcaa agttcctaat cttatctaaa ataacttaaa aattattaaa attatttttt
    18481 attgttttat tgtcttacac ctatattgtt aagcatttat cgtgccaact ttatatttaa
    18541 tcaaaatatc gcttgatttg aatgtttttt aacaatatcc aatcaaataa tctttttcat
    18601 atcgtggcat cttgcaacta tccattgcat tttgctattc tttctatctc tataaaagtt
    18661 ttttttatat atttttttat gaaaaagctt gacatttcaa aaaaagtttt cattgctaaa
    18721 ctcaaaaaca catgttatca ttgtaattaa tcagcaagtt agttaactaa tttcaaactt
    18781 agataatttt ttaataatat ttaatctaaa aaataacaat ttctttgata aatttttaga
    18841 gtaattatta cattaaataa aaaattacta aatatcaaaa taacacaggg aaaacaattt
    18901 caaagctaaa tttttaacct taaaaatttt gtataaaaaa tatagcctta tttaatacat
    18961 attattattt aatacatatt atttctaaat gaaaaattac cataataatt taataattac
    19021 aggagggtat tgatatgaaa atactagtat ttggagcacg cgattatgaa gaaccagtaa
    19081 taaaaaaatg gtctgaagaa cataaggatg ttcaagtgga tatttatcct gaaaacatga
    19141 ctgaagaaaa tatagttaaa gctaaaggat atgatggtat atctatacaa caaactaact
    19201 atatagataa tccttatatt tatgaaactt taaaagatgc tggggttaaa gttatagctt
    19261 caagaactgc aggggttgac atgatacatt ttgatttagt taatgaaaat ggacttattg
    19321 ttacaaacgt tccttcttat tcacctaatg caatagctga attagctgtt actcaagcta
    19381 tgaacctttt aagaaagact cctctagtaa agaaaaaagt ctgtgaaggt gattaccgtt
    19441 ggatagctga acttcttgga acagaagtta gatctattac agttggtgtt ataggtacag
    19501 gaaagatagg tgctacttct gcaaaattat tcaaaggcct aggagctaat gtaattgcat
    19561 ttgaccaata tccaaatagt gatttaaacg atatattaac ttataaagat tctttagaag
    19621 accttctaaa agaagctgac cttataacat tacatactcc tttacttgaa ggaacaaaac
    19681 atatgataaa taaagatact ctagctataa tgaaggatgg agcttatata gtaaatactg
    19741 gtcgtggtgg tttaattaag acagaggact taatagaagc actagaatca ggaaaaatta
    19801 gagctgctgc ccttgataca tttgaaactg aaggattatt cttaaataaa aaaatgaatc
    19861 caggagaatt aactgaccca gaaataaata aacttctttc tatggaacaa gttatattca
    19921 ctcatcacct tggtttcttc actagtacag cgattgaaaa tatagtttat tctagtttaa
    19981 gtagtgctgt agaagttata aaaacaggaa ctgctactaa tagagtaaat tagtatttta
    20041 taaatatatt ttttcattaa ttagctatta tctaacaaaa tagcctttaa tttcccaagt
    20101 atgagagagt cagctcaata tatattgagt agactctctt atattatttt ttatattaaa
    20161 cttatactct tcttattcat accaaattac atttatatta atttattcta actactctta
    20221 ctctacatat attttaccca tttttacttt tctaattttt tctaattata tacatttgaa
    20281 tgggtacaat ctgtatttta caaaaaaaac acttaacaaa ctatgaaaat tgtatgatta
    20341 atattaatat atttataaga aaacattaca attatgttag ctctggattt ttagggaggg
    20401 agtatattgg aaaaaatgaa taaagttact tctaaaaaaa ctacctatat tgttacttca
    20461 gcattatttg catccataat atgtctgaca atagcatata tactacatat cccagttggt
    20521 ggaaacaatg gatatgtcca tataggagat gcttttattt atctagctgc aactattttg
    20581 ccaacaaact atgctatagc tgcttctgca attggtgctg gtctagctga tttatctaca
    20641 ggtgctgcta tctgggtaat accaactata atcataaaac caatacttgt attatttttc
    20701 acttctaaaa gtgacaagat aataaataag agaaatatag ttgcttcgat tgtagcaggt
    20761 ataataggat tagttttata catgtttgct gaaggtataa ttattggaag ttttacaagt
    20821 gcatttgtaa tgagtttact tggcttatta cagccaatag gaagttttat tgtttttata
    20881 atcttaggta tggcacttga taaacttgat tttaaaaaac gttattttaa ttaagaattt
    20941 aaatttatgg agggaaaatt atgaatatct tatatacaat tgaaaatagt atctatgtga
    21001 atataactaa tacatgtcca tgtagttgtg ttttttgtat aagaaatgaa aaagatgaag
    21061 ttgcaaatag tggtagttta tggttggagc atgaaccaag tgtagacgaa gtaaaagaag
    21121 ccttcaataa atacaattta gacgattatg atgaaatcgt attttgtgga tatggcgaac
    21181 cattaatgag aataaatgaa ttgatagaag tagcaatgtt tataaaagaa aaaagtagca
    21241 taaagataag aataaataca aatggtctaa gtgatttaat acataacaaa aaaacagcta
    21301 ttatgttaaa agatgttata gatgcagtat caataagtct taatgctcca aataaagaag
    21361 cttataatag agttactcaa ccaaagtttg gagaaaaatc atttgattat atgttagatt
    21421 ttgcaagaga ttgcaaaaaa tatataaaag aagtggcttt ttcagtagta gatgaaatat
    21481 ctccaaaaga aatagaagaa tctaaacaac tagcaaaaaa acttgatata ccattaagag
    21541 ttagacataa gaactagcaa gtaaaattgt aaaacattaa taatcacctg aaaggtgttt
    21601 caggtgatta tttctcttgt attaacattt cacaaaatag atttcatacc acactaaaaa
    21661 tgtgtaaatc gtccaaatct ttctccaatt attagaattt ccactaatgt agtcattaaa
    21721 aatctcatta atctcatcta tgttaaagaa tttatctgct atctcactat tgaatttatc
    21781 tcggatatcc tgattatatt tgtcatcagc catccatata cggattggta caataaatcc
    21841 taatttattc ctaaaagcaa tctcttctgg taatactttt gatgcagctt tacgaaaggc
    21901 aattttattc tctccacctt tcactttata ttctgatggc attcttgatg caatatcaaa
    21961 gattcttgta tctgtaaggg gcattctgac ttccaatcca actgcagtac tcattttatc
    22021 tatattaaaa taaatgtctc cttccaacca tatttgaata tctatatttg acatattagt
    22081 aagtgtatcc agttcctctg tttcttcata aattcttttt gtcagtttaa taggaagcac
    22141 atcaggattg tatttcttaa gaatttttcg cttctcatct tccttcataa tgttcgtatt
    22201 cccaatataa aatgtcttta gattctcccc ataacacttt gccttatgat atacattata
    22261 tccaccaaag aactcatccg ctccttctcc agagtaacaa atatttgtgt tctcagctgc
    22321 tgccttgcat ccaatagcaa aaacaatagc agatgcatct ccagttggtt gctccatatg
    22381 gtacattaca taaggtactg acgcaaaata ctcttcaggt gtaattatat atctcgaatt
    22441 tttacatcca agaatatcag ttgtctgttg tgctaatttt gattcatcaa accactcttc
    22501 ctcataacca catgagcctg ttttttctat atctgacatt gccaatatat aagatgaatc
    22561 aacacctcct gataggaata actctgcagt ttcatcctct gattttatct ctggcataat
    22621 tttttgaagt gttgtatgaa tttcatctgt ccattcctca atggacttgc tgtagtcagg
    22681 atgaaattca ggtttccaat aacgctcaat acatactgtt ttgttctgat agacaaggta
    22741 atgtcctggc ataagctttt ttactccacg gaagaatgta ttttcacctg ctacatatgt
    22801 aaaagacata tacatctgta acatttcttc atttaactct ttcacaaatc ctggttgttc
    22861 cataagctta cgaatagatg aactatagag aagcttccca ttttcagtct tataataata
    22921 gaaaggtttt actccaaact ggtctcttag gcaaaaaagc ttcttttcca ctgtatccca
    22981 tagtgcaaaa gcaaacattc catgcatgtg atttgccata tcatatcccc aggttttgta
    23041 tgcttctact aaaattgctc gctcacgctc atctctacct aaagaagaat cgattcctag
    23101 ctcctcacag agaactttcc aatttctaat atgtccattg tattctaaaa tttttatcat
    23161 acatgtatct tttcgtgttg aatacttttc ttctaagttt atcatatgtg tatcctttca
    23221 tattaaaaca tttaattctt aaatttattg attgttttta tttatattta ttcacttata
    23281 atcattgaaa atctactttt ttcaaagtaa actctttttt aattagtgct aatatgctct
    23341 ttgttaaggt caccaatatg attattaatt attatcagtg ttataacatt gaatttttta
    23401 tttttcataa atattgccct cctttaaatt attagctaaa tataatatat cttatgttac
    23461 ttacatattc tttaagaaat gctgaagatt ttataaagtt ttctgtagta taattaatta
    23521 aaaatacaat tctagttata aagcatattt tctattaaac aaaaaatagt taaaataaat
    23581 tcataataac ataaacttat tttaactatt tgtatatttt agttataaat attgacttaa
    23641 atttttaaca ggatacactc ataaaataaa cacgataaac aataaaataa tatatcttag
    23701 tttctttata tactttgtct tctttcaatt ttctaccatg tagacggtta agataatatt
    23761 agtaattcac tctactgctt aactcgtgcg atatggacaa ccaagaaagc tatttcctca
    23821 tttgttaact tttttccata tttcttttct aaaaaatctc taacttttaa tgcaattgac
    23881 atttcttttg gatttgacct tttcatttga ttgaataata aatcatctac actacttaac
    23941 atgctatctg aaaaaaatct ttccacaaaa aattttatat gtgttataaa tctcatataa
    24001 tgcatacttt ttttatctaa ggttctattc aaagaaaaaa caaaaatatt aattatttct
    24061 ccaattactt tggcatattt aatcgtatca tatgaatttc catctgaagc catagcgttt
    24121 gcaaaatgaa aagcaatatt ggctgcttct tcttctggta attgaatgcc caatctctct
    24181 tctaccatgt aaattgcaac cattccaacc ttaaactcat ttggataata attttttatt
    24241 tcccaaaata ctcgatttgt aatttttatt cctttacgca tcctttcaat tgcaaagtta
    24301 aaatggtctg ctaatattaa gtataagttt ttattaaaag atgagttcaa taatttttct
    24361 gcctctacta ttatttcctg tgttatcttt aaaatgtcga cagaaatatc atccattaat
    24421 tctaaaaatt gttttgattt tgtattttca acagggacaa acatttgatt atcattggaa
    24481 ccacaaatat attctcctgt ttttttacca tatccaattc ctttccctag caaaataaat
    24541 tcattatcat ttgcatcatg aactaaaaca acgctcgagt tcaaaacttt ctttattatg
    24601 tagttcattt tatttgctca ccttctattg gtcatttaaa atctatacta ttattttcaa
    24661 ttactttttt ataccaataa aaactatctt tcttatatcg attatatgtc ccctttccat
    24721 aatcatcagc atccacataa atatatccat agcgttttga catttgtttc gttgattcac
    24781 tgaccaaatc gatacaaccc catgaagtgt atccccaaag ttcaacacca tcttcaataa
    24841 tcgcatctaa catacattta aagtgttctt ttaaataatc aattctatat tggtcatgga
    24901 cttttttatc ttcagttaaa atatctttcg ctcctaatcc attttcaact ataaataaag
    24961 gctttctgta tctatcatat aattcaatca aagatatgcg tagaccaatt gaatcaattt
    25021 gccaacccca ctcactcatt tctaaataag gattttttac agctaataac gtattccctg
    25081 atgtaacatc taatccagtt gtatcggctg cttcacaaga agacatatag taagagaatg
    25141 aaataaaatc tactggatac tttttcataa tttcatcatc ctgttcttct tttactattt
    25201 gaatgttatt atttttaaaa tatgacaata aatatactgg atactcacca aaaacctgcg
    25261 tatcactgta tgcataaata cttctcattt tttgttgggc tgccaaaaca tcttctggtc
    25321 tacatgtata tggataatat gttaattttg ttaacataca ccctactttt gaatttggaa
    25381 taatctcatg gcaaattttt gttgcaagag cactagctac aaattgatga tgcatagctt
    25441 gaaaaataac tccctcaaag tttttatcct taaaacggtc tcgaaccaaa ccaccagtag
    25501 tgtatggatg gcgaatcata gaatcaactt cattaaatgt aagccaatac ttcactttgt
    25561 ttttatatct ttcacagata gtttctacat acctaacaaa catattgata acttgtcgtg
    25621 aataccatcc atcatagttt aaaacgatat ttaatggtgg ttcataatgt gacatagtaa
    25681 caagtggttc aattccataa cttattaact catcaaatac atcatcataa aactgtaaac
    25741 cttcttcatt tggttctgta tcatctccat ttggaaaaat acgagaccaa gcaatactca
    25801 ttcgatatgt cttaaatccc atttctgcca tcagagcaat atcttcttta tagtgatgat
    25861 aaaaatcact accgtgacgt tttggataat ttacttcatc ttcaggatgt gctaatcctt
    25921 cttctatgtc tcttgtcgta atttcattat tggatttata gtctttcaca tccacatcaa
    25981 agtgtgcttt tgctacatct gatacagacc atcctttact acctaaattc caaccacctt
    26041 caaattgatt ggcagctgta gctcctcccc aaaggaatcc ttctggaaat tttctcatac
    26101 ttatattcct ccctttacat caaacaagat gcttcctttt gttacattgc cataagactg
    26161 atttaacact ttatagtcat aatctgcccc attactattt acaatcataa cagttgaatc
    26221 taaattagca tgtgagattt tgtctaaatc aaactgaatt aataaatctc cttcttttac
    26281 attatcgcct gtcttaacaa atgttttaaa tccatctcca ttcatactta cagtatttat
    26341 tccaatatga atcaaaatct cagccccatt ttttgttctt aaaccaattg catgttttgt
    26401 ttcaaatagc atcacaactt ctgaatcaca aggtgcgtat aaatttccat tggtcggtat
    26461 aattgcaact ccttctccca aagtcttact tgcaaataat tcgtcactaa ctttacttaa
    26521 atctatcact tttccttcta ctggagatac taatatattt acttcttttc cttctattgg
    26581 ctcttcttca acaacatttt caattgatga ctgttctata tttgtatctt ccttccaaat
    26641 aattagtgta ataataaacg aacctaatat tgcaataaca aaaccaatgc ttgcaaaaat
    26701 taaattattt ccattgtttg ggtcaataaa catgctcata ctagcaagcc ctggtccaac
    26761 aattacaaat ccttttactg taaataaccc taaaaatata ccagaaatcg cacttgacaa
    26821 tacaacacct agtattgctc ttttatgttg taaagtaata ccgtataaag ctggctctgt
    26881 aattcccatc aatgcagaaa ttcctgcaga taatgctgtt tgttttaatg tttcatcttt
    26941 tgttcgcaat gctactgcaa aacaaacacc actttcagaa atgttgtgag ctaatgaagc
    27001 tgtcatatat aatgcttcat aacccaactt tccaattgta ttaattgcat aaggtgttaa
    27061 tgctttgtgc attcctgttg caaccataaa tggaagaaca gcagcaagta atcctacaac
    27121 taagaatccc attttgttat ataggaataa aataatggtt gtaaaaattg tcccaacatt
    27181 atatcctaaa ggacctaaaa tagttagagt aattggaaca gtaattgcca aagacatcat
    27241 aggaacaaag aaagttcgaa ttggttttgg tgaatacttg ttaaaaattt tctccattat
    27301 tgacaagaaa gacacacata aaatagctgg gaaaacctgt gcattatagg caatacttgg
    27361 aatcgtaaat ccaaataatt cgacaccttc acttaaagca gtcgtcgttg ctggaagtaa
    27421 taagtatcca acagcagcaa tagctactaa acgattacat ttcaatacat tggcagtagt
    27481 tacagccacc atgagaggta agaaatagaa tgttgcatct gaaatagcaa ttaacaatgt
    27541 atatatagta gaatctgatt ttaaaatatt aaacattgct aataacatta acagtgattt
    27601 tagaatacca gctcctgcaa tagcgaaaac taatggttga aaaatattaa taataaattc
    27661 tatgaatctt gttcctaaat ttattttttc ttcagttttt atagcactat catttgtatc
    27721 taaagggcaa acttttaaaa tttcatcata aacatcaaca acactatttc caatgacaac
    27781 ttgaaattgt gccctatcaa ctatactcat aacaccttgt acttttttta aagcatcttg
    27841 attgacttta cttctatcaa tcaacttaaa gcgtagtcta gttgaacagt gttccaaagc
    27901 tagaatattt tttgagccac caacttgttc aataatgtca atggcagttt ttttataatc
    27961 catcttgtaa tctccttctc cataattttt tatgaatggc cattcaagat gcaaaaaagg
    28021 cagaacctat ataaacacct tgaaaaatca aagcaatata ttcgatcctg cctgcattac
    28081 cagtaacatg tcttctattt atactatttt gtactaccat attaacattt gcctaaaaga
    28141 attgtcaata cttttttaaa ttttctgata ctaactaata gcgcctttgt aattcatttt
    28201 tcatatcaat taaagtaaca gtagccaccc accaattagt atcaaaattg aacctggtat
    28261 tttttctttt atattttctt tgaatataaa atgagcaagt attaaagtta ttatcataga
    28321 tacttgagaa aatggttcag ctatactaag ttctagatta tttaaagcaa ttaataacgc
    28381 taaataagag tatccattaa tagcaccact taaaattgaa agcccttttt tattattaaa
    28441 aatctcccaa attacttttt gtttcttttt aaatgctaaa aatataaata aatttaaacc
    28501 tacaaaaaaa tacagtatag ttgaatataa tataggagat acttgtacaa aaaaagcttt
    28561 gtccaatact cttcctactg actgtaaaaa tatgtacaaa aacattaatc tacatggtaa
    28621 attgttaaaa attacagaga cattctttac aaaggagctt acttttttta atataaacac
    28681 tccaccaatc ataattatta ttcctgcaac ttttgctaaa cttaaacctt cacctaaaaa
    28741 tataactgat aaaagtagta aaaacaatga atttaaactg tttattggag taacaagtga
    28801 aacttctcca gttgctaaag atgtcacata tgctactgag ccaattgaat atactaaact
    28861 acttatataa catggtatta aaaaagacca atcacttact ttatccatta tactaaatgg
    28921 cagtaaacat actcctccta taaagaaaaa taaaaatgat gaagctatat ttttataaat
    28981 atctccttct tcatcaccca ataatcttac aactactctt tcaaaagcta aaagcactac
    29041 tctaattaat aatgcaaaat aagataatat catttttgac ctcgcttaaa aattaatctg
    29101 acttattgtt atattgattg tacaaaacta ccatataggt aaatcttttg gattttttaa
    29161 tattttatgc ttaaatacag gtatattaag tttttcacct aacttacggc attgctcctt
    29221 ataagtacct tttaacttat catgaaaaac tatgccatca atcccatctg caatactatt
    29281 tatacacttt tctagtttct caagtcccga ttcatcatca tacattccac ttacaatcca
    29341 ttgatttggc aatacatgcc caattgatat gttaaattct ttatttttta taatatctga
    29401 gaaagatttc tcaaaatgta cccctccaac acataataaa gtcttcaaat gttcatcaca
    29461 atcaaatact ctaattaatg attttgccaa tacctgtatt gctatactat tattaaaact
    29521 atcactacta cttcctatct caatgtccaa cataggtaca gaatattctg taattaattt
    29581 agattctcca ccatatgttg ttccagacca gtgggttgct tctgttaatg tagtaaattc
    29641 atcaagtgaa ttttcatttt ttatatcttc aatagcatta attaggtttt taaagtatct
    29701 tggatttgaa ttgccaaatt ctcctgttgg aacatctcca attgtatgag ctgttaggat
    29761 atgttctgga gcattttctc cctcatgcca atttacaact cctgcaaaat cataatctct
    29821 gaaatgtcta tttaaaattg gaagataata ttcataatca tgacttatta catattttgt
    29881 ttttacaaaa tgaaataagt tattgttttt atctttaaac tctaatacaa aattatcatc
    29941 aataacaata tcagtttgat ttaaatccat tatttcctca caacacttta ttacattact
    30001 agctactggg tctttcttta aatcactaca aaagaaaaaa actgcttttt tattattcat
    30061 ccataaatca cactcctata acttaaatat aatctgttct catacttaaa attatttaaa
    30121 tatcactcta gctccattaa attatttata cataatatac taccatactc attttagcta
    30181 tgcctttgcc tgagtttcta tatatatgtg atacatcagc tttaaatctt atagaatttc
    30241 cactagatac aacataatct tctccattta ttttaatggt tacctcacca ctaaaaacag
    30301 ttataaactc ttgtgttcct ggaagttcag catttgaatc taaatttcca ccctcctcta
    30361 tttcaatcat aaatatctca aaccttcttt tcgaatcaaa tggaaatatt ggatagctta
    30421 tatattttcc atcatcttca ataaaatggt ctacatcact tttttgaatc aattctatat
    30481 cactttcctg tctctccatc aatgatgtaa atgagatttt tagtccatta gttattttct
    30541 tcaaagtaga tatggtagga tttacctcac acttttcaat ttgagacagc atactcttac
    30601 ttacaccact aagttgtgca atcttatcta agctaagatg tttttctttc cttatctttt
    30661 ttaagttctc agcaattatt tcatttaaac tttgcataaa ttcctcctaa tatgcttaat
    30721 aaaaacaagt tattttgatt atttaataga tttagtaact taatacaatt tttatccatt
    30781 ataatgtaca ttatgtttaa tatacattac ttctttaaga atgtaaatag ttttctgaaa
    30841 ttttgtaaaa gtggacaaat aaaaagctag caacctatat ttttacaagg ctactagctt
    30901 ttattatcat aatatttcat tttaactata aaacttagat gcacctataa gctgtctctt
    30961 agaaacaatc ttagctaagt ttgctaatga agaaattttt atatctttaa aagaaactaa
    31021 aaatacaaac ttaaaatctg taaattcttg atttatattg tatatatcaa aataacatct
    31081 caaattatca aataagtaat tatcttgtaa atctgcttct atctcatcag tagcatcttt
    31141 taaatacgct cttacgtctt caaataaaac actgaaataa gattcatcta tttcttcacc
    31201 taattcttct aatatttctt ctatatctaa atcgttcata tcaaaagaat ttcctgtcaa
    31261 ctgacaatca aatactaata tattttcttg atttatagta tttgatatat cttgtacata
    31321 ttcaaaatct ggacgtaatt ctaaaatgtc ttttatttca ctactcttta catggatttt
    31381 tccttttcta ttttcaattt ttaaatctat catatgctcc ccctttcaat ctacaaatct
    31441 ttaaatacct tgttttataa tcttttcaat gtctatccaa ttattaactc taactatatt
    31501 ttgatgttta acatctttat tatagttagt atccagaagt attactttaa caccatcatc
    31561 agctagttgc actgagttta aagggttatc ttctataaat atgtcacaac tcagctctct
    31621 cgccttttca actttataat cacttcctaa taagtaaaca tctatatctg aaaatccttg
    31681 tctcgataac caatttttag ttacatcagt taaacactca tgtctagctg ttacaaaaga
    31741 taaattatgt cctttagata atgtgctaat tacctctgta gcaccctcta caatctttgc
    31801 ttcatcatat gaatgtatat acttattatt aaactctgca tatacatccc ttattcccga
    31861 accatacaat tcttcccaat tatgtgttat acactctgct tcagtgatat ttttattaaa
    31921 aatatcattt aggtatggaa gaaaatgata agggcttgtt attgtcccat ctatatcaat
    31981 acatatattc aatttgttca tttttacctc catgtaacat ttattgattt gattaatcaa
    32041 acattatagc acagctttaa ttataaatca aaactctaga tatgactata cctatataat
    32101 accaaaaagc ctgcatattt aagcaagctt ttcgtaaatt ttacattttt aactaatata
    32161 taagtttttc taaggttggt tctactccaa attgtgattt cagttcttta tattctttta
    32221 atgctttatc attatttgtt ctagatttta tagctcttat cattaaattt tttggtgtgt
    32281 caagtggaga tatgtattct actacagaag tatcatatcc attgccctcc aataacaatg
    32341 ttcttaaacc atcagttatt aagtctgcaa atctagcttt aaatacacca tgttttaata
    32401 ttggttccat agcttcatat ctatattgac ccaataactc cttatgacaa catggcacta
    32461 ctactatagc ttcagatttt gctctaattc ctaaacctat tgccatatct gtagctgtat
    32521 cgcaagcatg tagacttatt acaaggtcaa catccctatt aggtgtataa ttagtcaaat
    32581 cctcttttat aaatgacata tttttataac ctaaattttt agccatattt cttgatgctt
    32641 ctataactac atctgaataa tctataccta taaaataaca atttttcttc aatacctctt
    32701 ttatataaaa gtttaagaca aagcttaaat aagattttcc acaagcacaa tccaagatag
    32761 ttatacagtc cttatccttt atttctttta agatattatc tatcaattca acaaaatggt
    32821 ctatctgatt gtattttctt attttatcat tttttatctt accatctttt gtaagcactc
    32881 caatttcttt taaaagtgca tttgcctgtc ctacttttac ataataatct ctgtttttta
    32941 tttgagatgc tgtaaattca tctattttag gtacttcttc cttattatct aaatatttta
    33001 catttacctt cttattgtct ccttcaatta aaacaatagt tcctcgctca acatatttaa
    33061 acactaatcc atcataattt tctgcctctt tagaaatatt cttaagcaat tcattaaatg
    33121 tatataaatc tgattttcct ttaaaattaa atttatattt attatcttcc tctattgcaa
    33181 ttcctttaaa ctcttttctt ccagatttaa aaattacctc taactcaata aaaaaatccc
    33241 tgttatcaag atttctttga gtaatcccca taaaaaacat atttagtttt cctatactct
    33301 gcttattcat tgtaatctcc cttttaactt atatccttaa atcttattaa ttgatactat
    33361 ttgtttctgt gttatttatt tcttctttta aatcattcaa tctattttta aaactttcta
    33421 tctgcttatc tgataaatca agatattctg ttatcttccc tatatactta gggcttttag
    33481 tatttattaa tttcaaagca ctttccatag atttttctct aagttcatat gctttcttta
    33541 catcatctct agcattactc aatgtttctg tatacttctc cttagaaagt tctggaatct
    33601 gcatattttc atattctttt acaacttctt tacacttatt tcttgcattt gtaacatctt
    33661 tttttatact agataattta gttatatttt ttgcgatatc cttagtgtaa ggtatacatt
    33721 ctctcattgg aactaaagct gctctttcta tattataaat ttctttctta aaaattccta
    33781 tactttccat atctattttt tttatatctt cactattttt ttctttactt gtatatttat
    33841 tattttcttt tgtagtatct tcttgtttct ctgatttatt attcactgaa acattactat
    33901 cagagttatt cttttctttt atgttattac ttccttcttc gttgctttct ttattttgta
    33961 tatttttatt ttgtttgttg tttaaatttt tattatttga tatctcttca ttctttttct
    34021 tatcctgttt taaatcagta ctttcttttt ccttaaagtt tgatacatac ttttcgtcat
    34081 atgtctcatc agaaatataa ttgtgataat acatagattc ttttatttta ttaagtccac
    34141 tcatataaat attacttatc attattgttg tagccaatac tacataaata ctactataga
    34201 tacaagacct cttaaaattc ttgtaatttc tgaatatcac tactaaagga ataagatata
    34261 aaaatataaa aactaatatc cacctcataa gtcgtgtctc ctttctcaaa accgaaatcc
    34321 ctcaaatatt tatttttaat catgtatctt atagtattta ccaatttatg ttttttaatc
    34381 gtgaactttt attaaattaa attctgtata gtcacaagat gctaatgtat attctactcc
    34441 atttcgaatc cctttcaatc ccattttaaa agattcttta ccatgcaagt gaccataaat
    34501 caccttttca acattatatt cttcaaataa ctttgtaaat aaagattctt ctaatttatc
    34561 atttgtagga ggataatgag taatcacaat tatttttgag tgaccacttt tacgagcaga
    34621 ttctaatgat aacctaagtc tatgttcttc tcttttataa actttttcat cagtctcatc
    34681 aaacttaaca tcattaggac atatccagcc cctacctcca caaattgcat aatctttata
    34741 ttcataaaaa tttgtctgaa taaaacgcat attttcatat aacttattta aacttgtaac
    34801 ggttgtccac cagtaatcat gatttccttt tataagtatt ttctgacctg gaagtttgct
    34861 tattatatct aaatcttttt tagcttctga taaattaatt ccccaagatg tgtctcctaa
    34921 tactaagacc atatcttctt ccttaacaac ttctttccaa ttatctataa tcttcttttc
    34981 atgaccatcc caattacttc caaagatatt cattggttta ttgactgatg ttgaaaaatg
    35041 taaatcacct atagcataca aactcataat atcattcctt tcttgattac aataaatagg
    35101 ctgtctcttt ttagaaatta ttttccatta agaggcagcc ccattaatat ttagcaactt
    35161 ttctacaagt agtttttact gaactacttt atcatctacc ttagtatcct tatattgctc
    35221 tagtaatata tctatttctt gttttaattc caaaatttca gttatatttt tatctgtttc
    35281 agttctatca agttttcttt gatactgcat agctttatgt gtatcaccca agtcatataa
    35341 ctcttgtatt ctatttagag tatctctaat agctatagac ttcatttggt acatagcctg
    35401 agctttcatt atatcttctc ttatttcact catttcttgg tctaaaagaa aggcagcatc
    35461 agatgctctt ctaagtcttt cagcaagagt ctcaggtatg taatggtcat atttatattt
    35521 taatgaatgt tctattgtag cccagaaatt cattgcaaga gttcttattt gtatttcaca
    35581 tataatttcc tttgaacctg caatggagtt tatagggtat tttattataa catgatagct
    35641 tctataacca ctatctttgt aatttgtaat ataatctttt tctcctatta tagtcatatc
    35701 atttcttact tttatcaaat caactatagc atatatatcc tcaacaaact ggcacattat
    35761 tcttatacca gcaatatcat caatttcagt ttctatatct tttgcattta tcctttttaa
    35821 cttagatatt atggaagata ttttttttgt tctgcctgtg acaaactcta tgggagaata
    35881 ttcacctttt gctaaatatt cttttcttat atttttaaat tttattttca attcctctac
    35941 tgcatgttca taaggtgcta gtatctcatt ccatttttcg tattccatat tgctcaccct
    36001 ttatttgttt tttatgacct tctttatttt atcataattt attttgtttt tgtatagtaa
    36061 agcaattctc cattttccaa tatataccct atttcttcta aatcaactaa gtaacttatg
    36121 acagatacta acgaagattt aaaaaaatga tactctttat aattattgct taaattattg
    36181 ttatttataa tgttttttaa taaaatttct aaagatttcg gttctacaag caactctctt
    36241 atttgatgta aatatttttt taaagcattc aaatgttttt cgattaattt ataagaatct
    36301 tcttttgata taacttgctt gccatgacct aaaactaagt attcaaaatc tatatttttt
    36361 aatttttcta atgaatttat ttgttcttgt atatcatata gaaacaagaa gtcgtatttt
    36421 gaaagaattt catcccctat caataaatcg ccaacaaaaa tcacattatc cttcgtcaaa
    36481 acagctatac taccaggtgt atgcccctga aactcaatta tatcaaatat ttcattattt
    36541 aattcaattt ttccagcttt tatagattta tctatcttta tatcatccaa cattttattt
    36601 tttaagatat tatccataaa tacattgctc tttccaccaa caatatactt tgaaaataat
    36661 tctggatttt ctatatataa cttatcgtat tcagatgaca ttatacgcac atctttatag
    36721 tactctttaa attgattaca agctccataa tggtcatcat gaccatgagt atttataata
    36781 tatctaactt gtatattatt ttcatctaac actttattta ctctcctagg tcttgaacca
    36841 cataaccctg ggtctatcat aagtgctgtg ttatcttcaa atatatatat gcctgtattt
    36901 gtgccacctt ttataaaaaa ggtatttcct tttacttttg taagattcac cttcttcacc
    36961 tcattttgac aatatttatg atacaattta ttacttgcta atataaaact atacaagaat
    37021 aatccttttt attctatcta tagttctttt tcttatttcg ggttctttag atatccttat
    37081 tttcacatta tatgctaaat ttctataaag taatgactct ataaaatatt caactatacc
    37141 tctatcccta aagtctatat ctatacatcc acttgaataa atatttgagt ctaatgtcat
    37201 aagctcatca aaaaagctta atctcttaga tttacttaaa taatctttat ttggaataaa
    37261 tctatcacaa taatctatcc tgtaatttac tacttcatca tttatttcaa atccatattg
    37321 ctttttcatt aactctctat agttattatt tttctcaata acattatcta aatttttatt
    37381 ataatcctct tttttccaat gtccaaacat cctacaattt aatggtctaa cttcatatat
    37441 taaacatcta ttattattat ctttaaatgg acatggattc ttttttgtat actcctcaaa
    37501 ataataatct aatattttac ttatacagct tttttttaaa tcaactctat cttcaagata
    37561 acaaaatata ttcaaaaact caattaaatt tatacctact gactccatac aacaatttcc
    37621 acatcccaaa caatttccca ctggcaagga tttatataca tcatttagtt tctcaaagag
    37681 cttattttta atggaataat ctatacaatt taaaatatct ttatttttta tactagtcat
    37741 tattattact ccttgttata attattaagt taatacaaac aattattata acatattttt
    37801 taaatttaat cgcatatgga ggtattttat gaatagatat acaaaaataa ttaacatgat
    37861 gggaagttat tacacaaaag actttgaaaa agaaaaaaag aacgtaataa aagttagaga
    37921 agttaaagaa gatacagtta gaaaattttt cttacaaggt gactgtgagg ttcttgtagt
    37981 atttgaggat actggaaaag aaatacttat tgatgacttt tctcctgaag aagatatcaa
    38041 aaaatattta ggagcaaaat tcataaataa aaaaagataa ttatcaaaaa taattctaaa
    38101 attaaaagtt aataaatata aagttataat tctatataat tatagttaaa agttatcttt
    38161 tattgctttt ttagacttta agatagcttt ttttctattt tttgtaaaat agttccataa
    38221 ttcacattaa taattcacaa aatgatataa tataacaaat agataaattt ttaaatttct
    38281 aaatacataa ttaatatttt gcgaggtgat tttatgcaaa tttattttag agttaaggct
    38341 attggcaaac gtaaacctat gctaaaactt gccccttttg atgtatctga caacatcaac
    38401 tgcctaaaag aagctatctc tgaaattgta tctaaaaatg ttcttgaata taatgagaaa
    38461 atcccagaaa aatcaatagt aaacttctta actaatgaag aaattgaatc tcaagctaat
    38521 attggaagag ttggatttgg ctctatttac aatgagaata aacaatctgt agaaaaggca
    38581 atagaagtag cgttacaagc atttgaagat ggaatctaca aagtgcttat aaatgaagat
    38641 gttattgaaa aacttgattc tcctataaat ctgaaaagtg gagatatatt tacttttata
    38701 agacttacct tgcttgctgg aagaatgttt taaaataaat aacttggagg tacataaatg
    38761 ttagaacgta ctagagtaga aaaaaatcct aaaataattg aaaatttaaa aaacaatttt
    38821 actaaatctt cttcaaaaca gtattacgac ttagcaaaat ctctttggga ctgcatagaa
    38881 aatagttcat actattatag agaacctgaa gacaaattct taaatatgat aaagaaatac
    38941 tacaattgcc actactacga aattaaaaat atggacgagc tcctttctgg acctttaaaa
    39001 aatgcattag aattaatatt tacttctgaa caagtagagg aactttgcca attaattaaa
    39061 agaaagatgg attatacata ttcaggagga tattatcgta aaggatatcg ctcaaataaa
    39121 cttgtggatt attttagagg cattataagg tttatttcca gttatgttga tttgatttat
    39181 tatgattttt cagttataga ttatcttact cttccaaata aagtcttgta ctatgactct
    39241 ataatgagtg ataaaatagc actagaaata gacaaaggaa atcaagaagt aataagtgcc
    39301 ttagaagaag taatatttgg agaaaatcaa actgctctac ttacaagaca aatgataatt
    39361 ggtatcatga agagtcatag tgataaatgc tatgaaatga tttctaaact tcttttggca
    39421 gctaaacaac aagagggact tagacagcaa attgtagaaa gtttagatga gggtacaatt
    39481 tctgcatcaa tatacatgtt aaaaactatt ttggacaata atttagagcg atttagttct
    39541 gttataagag ccttggatac atggacagga cttggttttt cagaccaaaa acctggaata
    39601 atacgtaaat gtttagaact ttcatataaa tcattgactg atgaaaagtt ccgtatagaa
    39661 tcaattaata gcaatgataa tcttgaagtt tattttgcct tatgggctac tgcaacttac
    39721 aacatatatg actcttactc tcttattgaa aacttacttt cttgtcagga aaaatataaa
    39781 aaacttgtag ctctttactt tttatcgcat acagaaagta catattttaa acattctata
    39841 gcatctaaaa acattaaggg tgatgattta gagatactag catgggtaat tacaaatttc
    39901 tatgtagaag gttctgttgg ctactactat agttggcaaa acagagaaac tagttttgac
    39961 ctgtcaaaca ctcctattga caaagacttt aatgctagaa aagaacaatt tgaaagttta
    40021 aagcatactt tagaaaattt aaattcaaag acaaaatcat tttcaggaag tgtatttcca
    40081 tggacttgga ttgaaatttc ttctcatgaa atacttaagt gtatgatgac tttaacagca
    40141 tacgatatga atgctcaatt tatagatgtt cttatggaaa attgtcatct tatgagttca
    40201 gatttaagag cttcacttat agtaaatttt gtaaatgaac caaagacaga tagacaacgt
    40261 gaatttttat tggaatgttt aagtgataag agtgtttcaa atagagaaat agtattgact
    40321 ctacttagaa aacttgaact aacagacatg gagttggata agataaccaa tctattatct
    40381 ttaaagacaa gctctattag acaaacagta ataaaaattt tacttgaaca agatgattca
    40441 aaattggaga atattgcttc ttcactgata aaatcttcaa attctcaaaa atgccttgga
    40501 ggtcttgaaa ttgtaaactt attaaaagac aatactgata gaaaagaact atataaaaag
    40561 cttattttac tgataaaaga tattgataaa tctaaaaaga atacatctca agttcaaata
    40621 ctaattgata atttactagg taaagcaaaa aatgattatt gcaaagaaaa tggttttgga
    40681 atatataacc cagaaaataa ggttcaaatt ccagaacaat ttgattttaa agataattct
    40741 cctagtttat caaaacttga taaaattatt tcaaatacaa gtttatctac taatgaggat
    40801 tctgtttctg tagcaacaaa aaaagaagca aaaaaaattc tcgatacaga tataaataaa
    40861 ataataaaag tttatgaaaa atttgatgag cttattactg aaaatgcaga ttatgaatat
    40921 gaagttgaaa gatggtcagg taatgtagac aaagtacttt taggctattc tacttataat
    40981 ttgactccta ttaaatataa agtttctaat gatactctag ataattatcc attggcagaa
    41041 ctttggagag aaactagtaa aaaacttaac ttagactaca aattaatact tgatatatta
    41101 ttttatagta taagtacatg cggatataat gaaatagaaa aaagttggtt ttcatctata
    41161 tattcaaatg tccttgactc taaaaaacaa aataaaatat ctacaaaact tgaaaaatta
    41221 aaattcaatg gacttacaag aaagcttcta agagttttac taaatgagtt cgaccataaa
    41281 gaaatattta actattgttt ggaagtatac tataaactta gaaacttatt aactatagaa
    41341 caatgtaaaa agcaaagagt tgagtttact aaaaattatt atagacaaaa tgaatactgc
    41401 cctgctttaa atgcccctat tttaaattat tggcttgata aagcacattc atttatttat
    41461 gatgatattt cttttactaa gtttttctac actacatttt caaattatca gttgctagaa
    41521 tataaatttc ctttttatac tagcataaat gtatttgctc gtgcacatga attaggatta
    41581 atgactaaag agcaagttaa tagagaaatg ataagtggat acaacgcaac aaatataata
    41641 agaacgttaa caaatcctag atgggctggt gacacattag aaaaatatcc ttggcttaaa
    41701 gataacttaa aaaccgttac aaaccgaatt atagaaattg aatcacgtag aggtgatatg
    41761 ccaacagaag tgtctcatct agctaataat atacaatact ttgaaggtat aaattatttt
    41821 gtaaatatct tagttgctct tggtaaagaa actttccaga gaggatattc atatggtagc
    41881 aattattcaa aaaaagatgt tttaagttct ctacttaaaa attgctatcc tacaaaagaa
    41941 gatgattcaa aaaaattaaa aagtttactt aaaaataccg acattactga gttaagattg
    42001 atagaatctg ctatgtatgc acctcaatgg gtaaacataa ttgaaaaata tttaaactgg
    42061 aagggtctga aaagtacaat ttggtacttc cacgcacata taaatgaagg attctctgca
    42121 caaaaagaat cagaggttgc tctattctct cctatatcac cacagagttt catggatgga
    42181 gcatttgatg tagattggtt tttagattca tataaaacta ttggggaaga aagattcaat
    42241 atactttaca aatcagctaa atatataact agtggcggaa attctcatag acgagcacaa
    42301 ctctatgctg atgctacact tggaaaactt gataaagata tgctgatgaa agaaattgaa
    42361 acaaatagaa atcaagataa attgagaagt ttctctttac ttcctatgaa aaaaggagac
    42421 cttaaagaag caactttccg ttatgaattt attcaaaagt ttgccaaaga aagcaaacaa
    42481 tttggtgctc aacgtcgtga aagcgaatca aaagcttgtg caatagcaat ggaaaactta
    42541 gctagaagtg ctggttttga agatattaat cgtatgatgt gggctcttga aacagaaaaa
    42601 ttaaatgaag ttgcttatat attcaaacca aaaaatattg atagaacttc actttggata
    42661 gatgttgatg aaaaaggaaa aacatctata aaggttcaaa aaggtgataa ggctttaaaa
    42721 agtgtaccaa aagctattgc taaacatcca tatgtacttg aacttaaatc tgttcaaaaa
    42781 gatttaaaga gtcagtattc tcgtgcaaaa cagagtttag agcaagctat gaaaaatgaa
    42841 atagtattta cagttaaaga aattgccaat atctataaaa atccagttat ctgccctttg
    42901 attaaaaatc tagtatgggt atgtgatgat aatattggat atgtttataa tgataagtct
    42961 aagttcttta tttgcaatat agaaggtgaa atttataagt tgacagcgaa aaataaatta
    43021 aaacttgcgc atccagttca tttgtttgag tctggtaagt ggagtgagtt ccaacatgat
    43081 ttatttagta agcaaattgt tcaaccattt aagcaagtat tccgtgaata ttatagaata
    43141 aacagtgatg aattatctga aaaaactata tctcgtagat acgctggtca ccaagtccag
    43201 ccaaagaaaa ctgtagcact tcttagaaat cagctttgga ctgtcgatta tgaagaggga
    43261 ttacagaaag tattttataa agaaaatata gtggcaaaga tgtttgcact tgcagattgg
    43321 ttttcaccag cagatataga gcctcctact attgaaacag tagaattttt tgatagaaaa
    43381 acttataaaa gcttggaatt ggcttctata cctccaatta tattctcaga agttatgcgt
    43441 gatatagacc ttgttgtaag tgtcgctcat gctggtggtg ttgaccctga aactagccat
    43501 tctactgtag agatgcgtat agcaattgct aaagaacttt tattattatt aaaagttaac
    43561 aatgtcaatt ttgaaggctc tcatgctctt attgaaggta ctttaggtga gtattctgtg
    43621 catatgggtt ctggtattgt acataaaaaa ggaataggtg ctttgaatat acttcctgta
    43681 cattctcaaa gtagaggtaa gatattcctt ccattcgctg atgaagatcc taaaactgct
    43741 gagattatgt ctaagatact attacttgct gaagatagta agattaaaga ccctagtatc
    43801 ttaagtaatt tataagtata aaaactatcc ctaaaatata ttggactttc ttaaaaaaat
    43861 tagaaagtcc aatatatact atactgtaat ttatataaat taaatcataa ttacaaataa
    43921 tttttatata cacttctctt atacattatc tttatggtac ttattatgtc acacaaaaag
    43981 tatttttctt ctattttcac tagaatcttt taaccctata aaatctatat aaaaagtctt
    44041 tttctagaga tttgtttaat atggcatttt cttgaaaatt tataaacctt atctctaatg
    44101 aagatttatt tataggtata accttatgta taaatcttag atttacaagg tatataaaaa
    44161 gttataaagc tagatcttta gcgattgctg tgagtataat tcttgctaca tcactcgttg
    44221 ttggtattgg tactctatca agaagtgctc agcaagcaga tgtagataga ttgaaaagag
    44281 agcttggaac tgaccatgta tactttaaaa atataaagaa aaatcaatta aagtccataa
    44341 aatctagcaa agatattaaa aatctgggta taacatctta ctatacgtct acagatgtta
    44401 atgaaaaatt acctataaac atactatatg caaataaaaa ttatttgact agtcattcta
    44461 aacttataaa aggtcgattt ccaaaagcta gcaatgaggt ggtagtcgag aaatggattt
    44521 taaatagtat gggcttaaaa ccagagataa atcaaaacct tacttttaaa ttatatcaaa
    44581 aagaaaatcc agaaactttt aaagttgttg gaatccttga agatagattt atagagaaac
    44641 aaaagggaca atgtgaaatt ttttctaatc tcaatgaatc aaaactgaat gattttacaa
    44701 cttatattga atttaatgaa ggcagtgata taaatacaaa catagacaat ataataaaaa
    44761 atgctatgtt agatgagaaa agtataggaa gaaactcaat gcttatagaa tctactatga
    44821 aaaacggtac tttagataat gcaagtaaat acgttgcaat tgctatgagt ctatttgctg
    44881 gtattgtaat atatagtatc tatgttatat caatatacca aagagtacag gaatatggtg
    44941 tcttaagagc tattggttcc actaacttta ggattttcaa acttatgctt tatgaacttt
    45001 tcatacttgc tctaatagca atgcctatag gtatatgtat aggtatgggg ggtgcacaaa
    45061 tatttaatag gtctgtagga aatattcaat ttgaaggaaa tataaatgtt acaccttttg
    45121 ttattccaga taaaataatc ctcttatcaa ttgcatgtac aatactaata atactaatta
    45181 taagtttctt tacatactta aaaataagaa gaatttctcc tattgatgct atcagaaaaa
    45241 cttttggtgc tgataaaaat gtaaccacag ttaattattt aatatcaaaa ttaactttaa
    45301 atatgtcagt aacaaaatct atatctgcga aaaacatctt tagaaataaa aaggggttca
    45361 ttataattgt attatctatg agtataggag gtataatcgt cataaaagac aattataaat
    45421 attctttttt agatgtgcaa aacaaatcca ctcaagagca aacatatatg aatgcagatt
    45481 ttattctatc aaatgtttat ctcaatgaaa ttggtagcaa tacttctttt aaagatataa
    45541 aagggtttag tgatagtcag atagataaaa taaaaaatat agatggaatt gacaaagtca
    45601 aaactgctag tatcttaaac acaagaatag aggtaaataa aataaataga ttagattact
    45661 acgaagctat aaattcaaat ccttattata aactaaatcc tctgttcata aaaaataaga
    45721 ctactggcaa atatacacta aagcaaaaac taaaaggtta taatgatgag atgataaagt
    45781 cattagaaaa atacttagta agtggaaata taaacttgaa agaaatgaaa aaagaaaatt
    45841 tagctattgt atatgtacca gaaatttcta aaacacagaa atacacaaag acttttactc
    45901 ctggtgaagg aacaccagct atagatataa aagtaggtga tactataaaa gtcaaatatc
    45961 caaagggtgg aatggataca ggactttatg aaagtctaaa agataattat gaatacttag
    46021 aatatgaatt tagagttgga gctattataa gttatacttt tgcagataat ggtatgtact
    46081 ctggagatga cggtgtagat gtaataacga gtagcgaata tctaaaaaaa ttaactggtg
    46141 tagataagta taacttagtt tatgcagacc ttaatgaaaa tgcaaacaag aaaaaaataa
    46201 ataacctttt aggtaaaatt ggaagtgaag tttctggaac tacaacagta gatatgatgg
    46261 tagaaaaaga aaattctgat aagatgacat ctagaactat gatttatagt tatggcatag
    46321 tagcaataat gttcataatc agtgtactta acataataaa caacataagt tataacttaa
    46381 cttcaagaac tagtgaattt ggaatgttaa gagctattgg tatctctgag agaggattta
    46441 aaaatatgat actttatgaa ggaatattat atggtgtact ctcaagtata attactattg
    46501 tgtcaggact tataatacaa tttaaaatgt attacacgca gggctttgta tcatatggat
    46561 taggattttc aatagattac aaaatataca tactagttgt tgtagcaaat ataattgtag
    46621 gtattctagc tacatatatt ccatcaagga agataaataa aattagtata gttgaagcta
    46681 taaacattac agaataggag ggataattat gcctatattg gaaactatta atttaggtaa
    46741 gatttatgga aaaaaggaaa catctgtaca tgcactaaag aatgcaaatt taaaaataaa
    46801 taaaggcgaa tttgttgcta taattggacc aagtggaagt gggaaaagta catttctaca
    46861 tttagtaggt ggtcttgaga gaccaagtaa tggtactata aaagttgctg gtaaagatat
    46921 atgttgtctt tcagataaag aactagcaag atatagaaga caaaaagtag gatttgtctt
    46981 tcaacaatat aacctaattc ctgtattaaa tgtaaaggaa aatatagaat taccattaaa
    47041 gttagacaat aaaaaaatag ataaagaata tattgaagac cttataaatt tattggggct
    47101 taatgaaagg aaaaatcatc ttcccaacca attatctggt ggtcaacagc aaagagttgc
    47161 tattgcacgt gctttatctg caaaaccaag tataatccta gcagatgaac caacaggaaa
    47221 tttggacagt aaaactacag aagaagtcat ggatttacta aaaagttcta taaaaaaata
    47281 taaccaaaca cttataataa taacacataa tgaaaatatt gctagaaaag tagataaaat
    47341 tatatctata atagatggag agcttaaatt aacactctaa aactatttat agaaaagagg
    47401 atttaaatgt tattgatttt aggtaattta aatttaaact tggattttga ctacagaata
    47461 ataagagaag aaaatgatga tgtagatata ttcatagata ttaattatcg tagcttagat
    47521 atagatactg atggttctaa cttatttaat tctagaatac aatttccatt tgtaagagca
    47581 ttaattctaa ggctaaataa gaacaatcaa tgtatgacaa ttcatttgtt aagagatata
    47641 gatttattct ctgcttttgc caactttgaa gtggattata ctgactctat aataaatata
    47701 aaaaatcaaa atgagaaagt aattttaaat aaatctatca aaaaatagta agggggcctt
    47761 aataggcccc aatttttttc ttttctaaat ttattatatc atgataaata aattagtcaa
    47821 tagaattttc tgaatatttt tataattttt tcataaagtt ttttatatat tatgctttaa
    47881 aattatatgt aatagaaaat ttttccgact aattttaaag ttactttatt aaatacacat
    47941 attcaaaatt gacattaaat tattgacttt ttatcatttc aatttagaat ttaagttatt
    48001 atttataact atttatacgt atctctaata aaactctatt tatattcaag tccaataatt
    48061 tatatacaaa accaatagaa cctatgttgt acaatgaata ttatttacca taataaactt
    48121 ttagatatat ctcttttaat tcactcatca atgggtatct aggattagca cctgtacatt
    48181 ggtcatcaaa agcttgttca accatagcat ccagtccttc taagaattta gattcttcta
    48241 ctccagcctc ttttatagtc ttaggaagac ctacttttgc ctttagttca tctattgctt
    48301 ttataaggtt atctactttt tcttggtctg tatttccctt aattcctgca aaagaagcaa
    48361 attcagcata tctttgtata caatcaggat atctgtattg tgagaatgca cccatcttat
    48421 ttggagcttc tgcacaatta aacttgatta cttcatttat taataatgca tttgcaacac
    48481 cgtgttgaac atgatggaat gcacctaatt tatgtgctaa agagtgacat attcctaaga
    48541 aagcatttgc aaaagacatt cctgccatag tagatgccat tgccattttt tctcttgctt
    48601 ctttatcatt ctttccattt ttatatgcac gaggtaaata ttcaaatatt attttacctg
    48661 cttgtagagc taaaccgtca gctggttcag ttcttaacat agatacatat gcttctagtg
    48721 catgtgttaa agcatcaaca cctgatgacg ctgttagtct tggtggcatc tccatcatca
    48781 tatcagcatc aattatagcc atgtttggca ttaattcata atctgctaat gggtatttta
    48841 cgcctgaatc ttggtcagtt ataactgcaa atggagttac ctctgaacca gttcctgctg
    48901 aagttgggat agcagcaaag tatgcttttt cacccatctt agggaataca tatactcttt
    48961 ttcttatatc cataaatctc atagctaagt cttggaaatc tacttctgga tgctcataca
    49021 ttacccacat tatcttacca gcatccattg cgctacctcc acctacagct attattaaat
    49081 ctggttggaa atcagccata gctttagctc cttctcttgc acaagctaat gttgggtctg
    49141 gagctacatc aaagaatgta gtgtgcttaa ttcccatctc atctaataaa tcagttaact
    49201 tcttagtata tccattatta tataggaaag agtctgttac tataaatgct ttctttttat
    49261 ccataacatc cttaaattct cttgctgcta cacctaaaca tccttttttg aaataaactt
    49321 tttctggtgc tctgaaccaa agcatattct ctctcctttc agctactgtc ttgatattaa
    49381 gcaaatgttt cactcctacg ttttcagata ctgagtttcc accccaagaa ccacaaccaa
    49441 gagtaagaga tggtgccaat ttaaagttat ataaatcacc tataccacct tgtgatgatg
    49501 gagtattaac taatactcta caagtcttca tagcatttac aaacttttct actctctctg
    49561 gatgatttat agagtctgca tataatgaag atgtatgacc atatcctcca tctgcaacta
    49621 atttttctgc tttggcaact gcatcttcaa aacttttaga cttgtacatt gcaagtacag
    49681 gagaaagttt ttcatgtgca aaaggttcat ttaattcaac agattctact tcaccaatta
    49741 ttattctagc tgtttttgga acttctacat ctgccatttt agctattata tgtgctggtt
    49801 gtccaactat tttagcattt aatgaaccat caattattac tacttttcct actttttccg
    49861 tttcttcttt atttaagaaa tgtgcaccac gatatttaaa ttctttttta acttcttcat
    49921 atattttttc tggcacaatt actgcttgct ctgaagcaca tatcatacca ttgtcgaatg
    49981 attttgatac taatattgaa cttacagcca tttttatatc tgcactctca tctattattg
    50041 cagggacatt tccaggtcca actccaacag ctggcttacc tgaggaatat gcagatttaa
    50101 ccattccagg tccaccagta gctaatataa ggtctgcctc tgccatgaca acattagtta
    50161 attcaatcga aggaacatct acccaaccta taagaccctt tggtgcgcct gccttaactg
    50221 ctgcatcatg tacaactcta gctgcttcta tagtacagtt tttagctctt ggatgaggag
    50281 agaaaattat accatttcta gtttttagtg ctattaggga tttgaatatt gcagttgatg
    50341 taggattagt tgtagggata actgctgata aaacacctac tggttcagca attcttgtaa
    50401 aaccaaatgc ttcatctttt tctattacac cacaagtttt agtgtcttta taagcattat
    50461 agatatactc tgaagcaaaa tggtttttta taaccttatc ttcaacaata cccataccag
    50521 tctcttccac tgccattttt gctaaaggta ttctttgttg attagctgca gttgaggcag
    50581 ctaagaaaat tttatctacc tgttcttgcg taaatgttgc aaattctttt tgtgctaatc
    50641 taatttcttc taatctcatt cttaatgttt caacactgtt gacaatttca ggactacata
    50701 cttcaacact tgatttttct actacatttt cttttttctc catactacaa acctccctaa
    50761 aataattata catctacaat ttttataaat cttaaataca tcatctgtac ttttaactta
    50821 tacttaaaat aattttatag atttcatctc tataccttag attttattta ctttactttc
    50881 ttaggtcaag aagttttttt ctgaatattt tgtcaattta ttttttaaca aatatattac
    50941 tttttttact tttactaaat ttttttatct aaatgagata tatttcattt gaataattta
    51001 ctttgactaa ataaaatact aaaatgataa tttatatctt tcattttttt tacttattaa
    51061 attttatatg ttatttgtac aagcagtaaa taaaattttt gtcgtatttt tataaaataa
    51121 cacttatttt tgacttgtgt gcaatttttc tttttatagc aactgtatgg ttttaaaaaa
    51181 tatacaaact aaaattattc taatttctat aagttatttt taagtcaata tacaataatt
    51241 atacttttca tatggataaa taattt
//
LOCUS       DAFNNB010000008        49466 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0008, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000008 DAFNNB010000000
VERSION     DAFNNB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49466
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0008"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..385)
                     /locus_tag="L3M60_000411"
     CDS             complement(<1..385)
                     /locus_tag="L3M60_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6225115.1"
                     /translation="MNNIRINKFEKSCEKHWDYFVENKSINGTFLQTRNFLNYHEEGK
                     FTDHSLIIYKGEEILAVVPACEIYENGERIFFSHLGSTFGGIIIRSGFNDIRHVNAIT
                     NKLEIYLQEQSFNKVYLKNTSRIFCK"
     gene            complement(394..2508)
                     /locus_tag="L3M60_000412"
     CDS             complement(394..2508)
                     /locus_tag="L3M60_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBN6225116.1"
                     /translation="MNNDKICFITCVNDERLYEECLVYINSLDIPKNFSIETVALRGA
                     DSIASAYNKAMRETDSKYKVYLHQDTFIINKNFIYDILEIFKKNADVGMIGVAGVKDI
                     PVNGIWWDSDKKVGKVYDSHTGKMDIYEFSNFDECFNEVMAIDGLIMITQYDVFWRED
                     KFDGWHFYDLSQSMEFRQNKYKVIIPKQNKPWCMHDSGVANINNGYDEYRIRFVKEYI
                     NLDLDRENFYEFDEKKASTILKSNKHVMDCMKEELTEQLILKNYKDVLDIVSKIARFS
                     VFNHTGLYSCEKCERALIECANNLPEIENTKLFTKKDLKRRKVLHVLSEGYSTGGHTR
                     LVKNWIEIDKDSVHSVVTTWQSDTLPEWLLKAIDDSGGEIISLDLISDDYLKRAMALR
                     KIAYDWADIVILYVHMWDTIPVVAFGVDGKIPILHVNHYDSTFWLGASIADIVIDFSE
                     ASQLITLNKRGIDKSEILPILLKEKEVFSKSEIRKQYNLEDDVTIILTIASERKYNSV
                     NDINYFKIAKTLIENTDNTIIIVVGVDTENIYWKTLSNVTDNRVICFGTTTNIDDFYK
                     ISDIYLESFMVGSHTSKLDAILYDLIPVKFKNEVSPIFTNMWNFIDRFDYKNIKEVID
                     MIKRYNTSEFDRNKIKEKQSGIKHFVIDKHCIEIKNYIDKIYNEANHHKVKCDLYKND
                     EIEEYQLFVALYGYMVKNKNMS"
     gene            complement(2501..4378)
                     /locus_tag="L3M60_000413"
     CDS             complement(2501..4378)
                     /locus_tag="L3M60_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBN6225117.1"
                     /translation="MNTPILVNEKSKDIIKDWQYTYTNEDIKFTSIIILTYNQIEYTK
                     LCIESIRKFTPKDKYEIIIIDNNSSDGTVEWLKGQSDIKSVYNSKNLGFPKGCNQGIE
                     LANGENILLLNNDVIVTPNWLYNLNKALWSQDNIGAVGPITNACSYYQQIDVDYKSVE
                     EMFIFAQKINKSNINLWKYRLKLVGFCMLIKKSVIDNVGVLDELFTPGNFEDDDLSFR
                     MIESGYKLLLCKDTFIHHFGSISFKENRNKYTDIMDINRRKFETKWGFNPEYSGIIRT
                     DLISKIDDSPETNINVLEIGCGIGATLLEIKNIYKNASIYGIEISESAGKLAKYSADV
                     LIADIEKVKLDYKKNFFDYIILGDVLEHLIDPWKIVNKLKQYLKKDGYLVSSIPNVMH
                     VSVLRNLVLGNFTYEDSGILDKTHLRFFTLNEITKMFNDSNYIINSITAISMPLSEID
                     NRFIDTMCKESCKEMRQQYESYQYIVKATKSIDTKKYSSISMVNLKYALMRIDNELDI
                     DGNLSYIFDNYNITEPCFVDDIEYLVDTFVINKVTLLNRLGVEAFNRGLGDFSINMFM
                     KVLEMDRDDIDSIYNITSVLCELEEFDIAYNLIKNSSEKVQSESDIQEILYFIEGKRY
                     E"
     gene            complement(4402..6543)
                     /locus_tag="L3M60_000414"
     CDS             complement(4402..6543)
                     /locus_tag="L3M60_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBN6225118.1"
                     /translation="MLLSIVMIVKNEEKILEKTLKSLTTLRDSIESELIIVDTGSTDD
                     TIKISKKYTEKVYFHNWNNDFSSMRNISISYAKGEWLLILDADEILIDSSTIVKFFKD
                     GLDKKFNSASIQLKNLYSYDKKLYGYCSVLRLFKNVDFRYHGKVHEQPIYKNPIFNNV
                     ADFEHYGYLFEDEEIRLNKVKRNEKLLFEELKENDNSPYTNYQLGKNFIILGKYQEAL
                     YYLEKSYELYSKLESAPGYLITSLVKTYLYLGKHKKCEKLCLKYIKKDMNNIDIYYYI
                     AQAQVDLGKYENSIDSYKRYIYLLDNYEISTQANSLFSDTDTVGLRDEAIITLIKIYY
                     KLERYESVISEYNNIEDIEKKKNVYFSLFMSLYKLNRFEDIKNYYKEVSNSKIEKNSF
                     YRNIEEVINNIKEDEKEYVYKILCDIEGNYGILNQIRINKCVSIDKCKEILNEEKEII
                     YAYIIKIAFEEKLDLLDIFYEMEYVWIEKYLKYLLALERSFNLALYKYIISCPNTSDM
                     EKIRVYKAISKVVLESISLSDEKYKEIFYLYIMYSYKYIRYIYSNLSDFELIKFVCND
                     LDKFTIEFKHLLNIKNKVNSANEKLEYIHSMRKLLNEYPFYNKIIKILIGELEENIKE
                     SEEFRDLKDSFIISIENMIETGKINDAKLLVDDYSKTFVDDVKILNIKGILYMFENKL
                     DKADFMLKKALSLDVENEDTIYNIEYLKSLR"
     gene            complement(6636..7508)
                     /locus_tag="L3M60_000415"
     CDS             complement(6636..7508)
                     /locus_tag="L3M60_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HBN6225119.1"
                     /translation="MRVNTNVSALIANNQMGRNVSAQSKSMEKLSSGVRIKRAADDAA
                     GLAISEKMRAQIKGLDQAGRNVQDGISVVQTAEGALEETGNILQRMRTLSVQSSNETN
                     TAEERQKIADELLQLKDEVERISSSIEFNGKKLLDGSSTEIRLQVGANFGTNVAGTTN
                     NNNEIKVALVNTSSIMSKAGITSSTIASLNVDGASGTDAAKQMVSSLDVALKELNTSR
                     AKLGAQQNRLESTQNNLNNTIENVTAAESRIRDTDVASEMVNLSKMNILVQASQSMLA
                     QANQQPQGVLQLLG"
     gene            complement(7609..7944)
                     /locus_tag="L3M60_000416"
     CDS             complement(7609..7944)
                     /locus_tag="L3M60_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HBN6225120.1"
                     /translation="MDKLKEKLNLYKDISLQIINLIEKEEYIKISSKLDERQEIINSV
                     SEIDRNDFIQLYNKMELIEIDSRIRDILQGQLLEVKKELHEYKLTKQVNTMYYSLNRE
                     KVNIFNKKV"
     gene            complement(7947..9470)
                     /gene="fliD"
                     /locus_tag="L3M60_000417"
     CDS             complement(7947..9470)
                     /gene="fliD"
                     /locus_tag="L3M60_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBN6225121.1"
                     /translation="MSSISPIRVTGLSGNFDMEGIIEASMIRDKEKVDKAKQEQQIVK
                     WKQEIYRNIIQESKDLYDKYLSVDSPNSIVSQKAYSSTKITSSDESIIVAKGSAGAEK
                     INYQFAVSQMAEPAKFTIKLNSSEPIVQQFPPNASGASSLTIGDVNIPISEQDTTSTI
                     VSKINSLCADNDIKASYSEMTGELIISRKQTGSSSDINLRVIGNDNLAQQIANDNGIT
                     FVSDAGGNKLASVYGKNLEADVTDEHGRVTHISKEQNSFNIDNIDYNVNSKGTAKLTS
                     VTDTEEAVKNMKSFVDDYNKLMDKVYGLVTTKKPKDYPPLTDAQKEDMTTEEIEKWEK
                     KAKEGILRNDDELRGFVEDIQSAFFGDGKNIIALRKLGINESENYNKKGQISFNADTF
                     SKALIDDSDKVYKSLAGYSSNYDDKGMFEKLKDIVYEYSGSSTSKLPKKAGIEKTASA
                     SENVYSKQIAEQERNISRLVEKMNDKEKRLYAKYSALESLLNQYSSQMNYFSQAQGN"
     gene            complement(9491..9847)
                     /locus_tag="L3M60_000418"
     CDS             complement(9491..9847)
                     /locus_tag="L3M60_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBN6225122.1"
                     /translation="MELNLAELGKLSKEELLLMLVDATVKYTNISKDALLNNDYLKAH
                     NELVRVQNIFTELMTTLDQDAGQWAKDMYKVYEFIKSELAIADENKDIKIIDDILPIV
                     KQIRDTWHEVYNKIKI"
     gene            complement(9847..10242)
                     /gene="fliS"
                     /locus_tag="L3M60_000419"
     CDS             complement(9847..10242)
                     /gene="fliS"
                     /locus_tag="L3M60_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBN6225123.1"
                     /translation="MYGENPYNSYKQNAVFMASKEQLLLMLVDGAVKYTKIARAAILD
                     KNTQRAHRELVRVQDIFTELMVTLDQNAGQWAKDMYKVYDFVRYELSRANIRKDIQII
                     DNVLPVIEQIKDTWHEADKKSKEERSRYK"
     gene            complement(10264..10476)
                     /gene="csrA"
                     /locus_tag="L3M60_000420"
     CDS             complement(10264..10476)
                     /gene="csrA"
                     /locus_tag="L3M60_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBN6225124.1"
                     /translation="MLVISRKKDEAVLIGENIEVKIVGVDGNNVKLAISAPNNISILR
                     KEIYEKVKSENIKATNKNINILKSLK"
     gene            complement(10470..10862)
                     /locus_tag="L3M60_000421"
     CDS             complement(10470..10862)
                     /locus_tag="L3M60_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliW"
                     /protein_id="HBN6225125.1"
                     /translation="MMKVTLKKGILGFENLKEYELLDIEDNDSLKEFNSTEEEGIGFV
                     VVSPFEIINEYEIVLNQETIEKLEVKSPKDVMLFNIITLGQTFEESTVNMKAPIVINI
                     KTNFGMQIILQDDKYSIRHPLLRGDSGC"
     gene            complement(10879..11811)
                     /gene="flgL"
                     /locus_tag="L3M60_000422"
     CDS             complement(10879..11811)
                     /gene="flgL"
                     /locus_tag="L3M60_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBN6225126.1"
                     /translation="MRVSTGMMSSSYLNSLQDNLQRLDKVNRQVNTTKEINKLSDNPY
                     KAIKILNSKSEIKTMEIYVENCKDTGNWLETTDTALDQLGKLLSDIKKTLVASGDGAY
                     SDDELKTLSNETNEKIKEIANALNATHEGKYIFSGSNTGIPPVECVDNADGSVSLKFN
                     SSIDLNKLKDSLSMDVAQGISVDYNVKLSSLGFDPTKTDPFEELNNISKKLANPDADA
                     IKELTTTCLGEMEQLIENTVNVRSIYGTKANTVEAMKEKNDDSLIQLKEILSQNEEID
                     YGEKLVQLKAAELTYQASLQTGGKLFSVSILDYI"
     gene            complement(11830..13140)
                     /gene="flgK"
                     /locus_tag="L3M60_000423"
     CDS             complement(11830..13140)
                     /gene="flgK"
                     /locus_tag="L3M60_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBN6225127.1"
                     /translation="MAGLFSILNTANSGMNSHQKAIQTISHNISNLDTEGYSRQRTEF
                     ATNSPMYMPSLSNSIGRGQLGTGVHVTDVTRARNSFYDYQFRAEAHKYGKTVSKYDYY
                     NTIETILNEPSDYGISAGIDDFFKGWESLSKDINSDSKKSLVVENASNLATLISESYK
                     KLDKLKGDIDSNIDTEIKQINEMLKSLEDLNKSIDIISGSGSMPNDLLDERDRILDNL
                     SFKLDFENSDVKNMLADGKLELNELKNADGTWKTGVSGTLQGLFEMHGKIDTYKSDLK
                     DVSDGLAKQINDVYNTSAGITVRDFFVTSNVDGEDIIKVNPAIKSNPNELKLTADEAS
                     KIAKLKDEKIDIGVAGGKVSTISDHYKAFAELVGLDSKKVNQDEVNQRKIINNIDNSR
                     MSVSGVSLDEEMTELIKIQRSYQASAKVMSTAVQLLDVVINGII"
     gene            complement(13158..13574)
                     /locus_tag="L3M60_000424"
     CDS             complement(13158..13574)
                     /locus_tag="L3M60_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HBN6225128.1"
                     /translation="MITDLKVIMYEEKNIISKMMELLKEQFDYIVEKDLENLNMLNPK
                     LEDISRELASIEIKRRQLFGDDISMSEVVENSKDGYLKEIYTDLKHILALAKNQQESN
                     DSLIKKELIFTKKMINFIKPVDKQTTTYNSYGNIRK"
     gene            complement(13579..13854)
                     /gene="flgM"
                     /locus_tag="L3M60_000425"
     CDS             complement(13579..13854)
                     /gene="flgM"
                     /locus_tag="L3M60_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HBN6225129.1"
                     /translation="MDIKNVSSNIVVNNYLKNTSGVKKVEDVKKINKTDKIELSKFAK
                     EMASVSNMSDESIRNKKVDEIKNALENGTYKIKPKEIAKKMIEDMRG"
     gene            complement(14006..14320)
                     /locus_tag="L3M60_000426"
     CDS             complement(14006..14320)
                     /locus_tag="L3M60_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HBN6225130.1"
                     /translation="MNSKKEDIYELNFGQLIEEQSGIIEENQNVFNAKVNVAVSIGNT
                     TETIKNILDLKEGSIIKLNKNVDEKLDIYANDRLFAYGESIITNEKLSVRLSKIEDSD
                     IN"
     gene            complement(14344..14772)
                     /locus_tag="L3M60_000427"
     CDS             complement(14344..14772)
                     /locus_tag="L3M60_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaaR family protein"
                     /protein_id="HBN6225131.1"
                     /translation="MGINIDKLKISPSINIEVKKLKENNKFSDRLNSLKQSSSLEDIK
                     RNLESVKKAGERLVLTQNYGDISIYKNAVKEYLKSVVDNMYSLDKNSSFWEHQYYKNV
                     KIVDSKLEDMTSKLISEEKENIDIVSTVDMIQGLLIDMYR"
     gene            complement(14781..15449)
                     /locus_tag="L3M60_000428"
     CDS             complement(14781..15449)
                     /locus_tag="L3M60_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN6225132.1"
                     /translation="MNEISSLINILALTQLSGSNNTNQCNCTYNNSSGFDMIMMTLLK
                     ALSQNNQHTSNGYNISSNSENLFNELENVVNNTTSRFIDVDTKDKNVKLRIENAVEQA
                     SKKYNVDANLIKAIIKVESDFNPNTVSSAGAKGLMQLMPENCRDLGVTDPFNIEQNID
                     AGTRHIKEYIDMFGGSIEMGLMAYNGGPGRMKSRGVESISDLYKMPKETQNYIPKVMK
                     YYRG"
     gene            complement(15487..16470)
                     /gene="rfbB"
                     /locus_tag="L3M60_000429"
     CDS             complement(15487..16470)
                     /gene="rfbB"
                     /locus_tag="L3M60_000429"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBN6225133.1"
                     /translation="MKKILITGGAGFIGSNFVHYMLNKYVDYFIVNLDLLTYAGNLET
                     LIEIQDNSNYKFVRGDISNREQMYKLFEEERFDIVVNFAAESHVDRSVENPDLFIKTN
                     IIGTQVLLDASIKYGVKRYHQISTDEVYGDLPIDRKDLFFTEQSPINPSSPYSASKAS
                     ADLLVSSYYRTYGLLTTISRCSNNYGPYHFPEKLIPLMILNALENKQLPVYGNGENVR
                     DWLHVYDHCTAIDLIIHKGNIGEIYNIGGHNERSNLKVVKIILNLLGKSEDLISYVND
                     RPGHDLRYAIDASKIQNELGWKAKYDFDFGIKETVKWYIENESWWKSVLSV"
     gene            complement(16508..17065)
                     /gene="rfbC"
                     /locus_tag="L3M60_000430"
     CDS             complement(16508..17065)
                     /gene="rfbC"
                     /locus_tag="L3M60_000430"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HBN6225134.1"
                     /translation="MGKINVIKTKIEGLYIVEPSVFCDERGYFMESYNKRDFIEQGLD
                     MCFIQDNESSSRKGVLRGMHFQVRYPQGKLVRVTEGEVFDVAVDLRKDSKTFGEWEGV
                     ILSDKNKKQFYIPEGFAHGFLVLSEKAVFNYKCTNYYYPEFENGFIWNDKQISIEWPL
                     KGIEEIFLSEKDKKQKSFKDICIRR"
     gene            complement(17114..17983)
                     /gene="rfbA"
                     /locus_tag="L3M60_000431"
     CDS             complement(17114..17983)
                     /gene="rfbA"
                     /locus_tag="L3M60_000431"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBN6225135.1"
                     /translation="MKGIILAGGSGTRLYPITKCISKQTLPIYDKPMIYYPMSVLMLA
                     GIQDILIISTPRDIKAFEELFGDGSSLGLKITYAIQEKANGIAEAFIIGEEFIGNDRV
                     SLVLGDNIFYGYGFSERLEKAVKRKGATIFGYHVSNPNSFGVVEFDKDFNVLSIEEKP
                     TVPKSNYAIPGLYFYDNDVVEIAKLVEPSDRGELEITSINSEYLKRGKLKVELLGRGM
                     AWLDTGTHKGLLDASNYVEVVQTRQGLYIACLEEIAYKKGYISRAQLMELAKPLIKTE
                     YGQYLLKLASEDM"
     gene            complement(18092..18964)
                     /gene="rfbD"
                     /locus_tag="L3M60_000432"
     CDS             complement(18092..18964)
                     /gene="rfbD"
                     /locus_tag="L3M60_000432"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HBN6225136.1"
                     /translation="MKILITGSNGQLGKELVNQLEAINQSMSQPKYAILATTRNDFDI
                     YNQTNVDNFILNNKPDVVVNCAAYTKVDACEDNIETAYKVNALGVRNLAIASEKVNAK
                     LIHISTDYVFNGFSKYPYREDSKTEPNSVYGKSKLMGEKFVEQFSHKYFILRTAWLYG
                     NGNNFVKTMIKLSLENKEVNVVNDQFGTPTSTVDLTKIIIRIMETEHYGVYHATCEGE
                     CSWYDFAKKIFELKNIDIKVNPIKSNEFKCKAQRPLYSVLDNFMLKLIGLNSFRNWEE
                     SIEEYLERYDMNEE"
     gene            complement(19168..24249)
                     /locus_tag="L3M60_000433"
                     /pseudo
     CDS             complement(19168..24249)
                     /locus_tag="L3M60_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013725947.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
     gene            complement(24260..25792)
                     /gene="purH"
                     /locus_tag="L3M60_000434"
     CDS             complement(24260..25792)
                     /gene="purH"
                     /locus_tag="L3M60_000434"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBN6225137.1"
                     /translation="MSKRALISVTDKTGVVEFAKELNKLDYEIISTGNTFKTLKENGV
                     NVMQVEDVTNFPEILDGRVKTLNPYIHGGILYKRDKESHVETVNEHKIHSIDLVAVNL
                     YDFEGTLKAGKSHDEIIENIDIGGPSMIRSAAKNYKDVIVVVDIKDYDSIIEKLKTDT
                     MTLEDRKKLSYKAFSTTGRYDALISSYFAGEVGDTYPDILNLTFQKEQTLRYGENPHQ
                     NGFLYSQSNAKNPILNYEQLGGKELSFNNLNDLHGCLEVMREFKDSEEVVSVAIKHAN
                     SCGVGLGKDAFEAYTKCYEADKVSIFGGIVGITSTIDKATAEKLNEIFLEIVVAYDFE
                     PEALEILKQKKNLRILKLAKIENSLQPYEMKYLDGKLLIQDRNNILAEKSENVTKAKP
                     TDAQLKDMEFGMRVVKNMKSNAIAIVKNGQTLALGCGQTSRIWALKNALENNKDKDFT
                     GAVLASDAFFPFDDCVTLAHEYGISAVVQPGGSIKDKDSIEACDKYDMVMVFTGIRHF
                     KH"
     gene            complement(25785..26378)
                     /locus_tag="L3M60_000435"
     CDS             complement(25785..26378)
                     /locus_tag="L3M60_000435"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBN6225138.1"
                     /translation="MLNIGVLISGGGTNLQAVIDGTESGEIKGQVKVVISSKQDAYGL
                     ERAKNHNIKAICETDEDKIIEILKENKIDLVVLAGYLKIISPKLVNEFRNKMINIHPS
                     LIPSFCGAGFYGEKVHQGVIDYGAKVTGATVHFVDEGADTGPIIMQDVVKVNQDDDAK
                     TLAKRVLEVEHRILKESISLFCENKLKLQGRRVFINE"
     gene            complement(26372..27448)
                     /locus_tag="L3M60_000436"
     CDS             complement(26372..27448)
                     /locus_tag="L3M60_000436"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBN6225139.1"
                     /translation="MLTYKESGVDIDEGNRAVDLIKGKIKGTYDGNVVGDLGNFSGLY
                     SLKDFVGMKEPVLLASTDGVGTKLKIAQMMDKHDTVGIDLVAMCVNDLICQGAKPLFF
                     LDYIALGKLVPEHIEKIVGGIADGCKMSGCALIGGETAEMPGMYGEDDYDLAGFSVGI
                     ADKEKIVSGNNVKSGDVLVGISSSGVHSNGFSFIRKIFLETYNYKMEQYVEELGMTVG
                     EALLTPTKIYVKLALDVLAKHEIKAIAHITGGGLIENITRVIPKGLGLDINKKSWEKP
                     PIFKMIEGFNAVNERELHKSFNMGIGLVLIVDKANADDVVNFINNRENDNAAYVDKKY
                     SELLEDKAYIIGEVVDSHEGVELC"
     gene            complement(27442..28809)
                     /gene="purF"
                     /locus_tag="L3M60_000437"
     CDS             complement(27442..28809)
                     /gene="purF"
                     /locus_tag="L3M60_000437"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBN6225140.1"
                     /translation="MCGVLGIYSNKDVTKELYYSLYSMQHRGQESCGLALLDDGEIKY
                     KKDMGLVGDVFKESELSKLKGNIGIGHVRYSTAGGSHVSNCQPLVGSCRKRQLAIAHN
                     GNLVNANYLKDMLEEDGYMFQTNSDTEVILYILARYYKGDIVESLKVTMDYIKGAYAL
                     VIMSQEELVAVRDPHGFRPLVLGKKGDEYIFASENCAIDILGGEVIRDVEPGEIIVVK
                     DGELKSYFYSENYKPVKKSCIFEHIYFARNDATIDNVNAYEFRIKCGERLAQNETVKA
                     DMVVPVPDSGWPGAIGYANASGLKISEGLVKNRYVGRTFIKPTQEEREIAVKIKLNPL
                     STIIKGKSIILVDDSIVRGTTSKQLVKSLREAGAKEIHLRITSPPVAYSCYYGIDTPN
                     RSKLIASSNNVEEMREYIGCDSLKFLDIEGMLDATEHKSTFCKACFDGEYPVKKIDKE
                     ELLSC"
     gene            complement(28821..29519)
                     /locus_tag="L3M60_000438"
     CDS             complement(28821..29519)
                     /locus_tag="L3M60_000438"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBN6225141.1"
                     /translation="MLLYEGKAKQVYSTDNENEYVVYYKDDATAFNGEKKAEISSKGI
                     LNNKISTIIFEMLKENNINTHFIKSLSDREMLVKKVEILPLEVIVRNIAAGSICKRVG
                     LEEGVVFDEPIFEISYKNDAYGDPMLNDDYAVAMKLATREELKFLREETLKINELLKA
                     FFLKLNLKLVDFKIEFGKDSEGNIILADEVSPDTCRLWDVNTNEKLDKDRFRKDLGDL
                     VEGYTEVLSRMNNK"
     gene            complement(29525..30001)
                     /gene="purE"
                     /locus_tag="L3M60_000439"
     CDS             complement(29525..30001)
                     /gene="purE"
                     /locus_tag="L3M60_000439"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBN6225142.1"
                     /translation="MKVAVVMGSKSDYPKLEEGIKLLEKYGIEVVARALSAHRTPEQL
                     STFLKEIEDDTDVIIAAAGKAAHLPGVIASQTLIPVIGLPIKSSTMDGLDSLLSIVQM
                     PKGIPVATVTIDLGLNAALLALQIMTLKYPKLKEDLKSYREEMAQKVLEDDKNLRG"
     gene            30451..30963
                     /locus_tag="L3M60_000440"
     CDS             30451..30963
                     /locus_tag="L3M60_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6225143.1"
                     /translation="MLEVIKNRRSIRAYINKSVEEEKITEILKSAMQAPSSKNAQPWE
                     FIIVDDKELLKKLSKSQHRAKHIEFAPLCIVVLGNRDKFLKPGKWIQDLGACTQNLLL
                     EVTNQGLAACWVGVFPKNKVVNKVRQTLDLPVKLVPYSLISIGYSEEKNEFIDRFDEN
                     KIHRNIYKNR"
     gene            31513..31728
                     /locus_tag="L3M60_000441"
     CDS             31513..31728
                     /locus_tag="L3M60_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225144.1"
                     /translation="MRILSETEKISLAAVIKMESDGLLMQRAINMLISDEDLKRQSES
                     SILATEGRIKAIQQFIVENEILISEEV"
     gene            31734..32027
                     /locus_tag="L3M60_000442"
     CDS             31734..32027
                     /locus_tag="L3M60_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HBN6225145.1"
                     /translation="MRNMIENLIKNNTYIDDKVIMLSMLSSAKASANMYLNSALTSST
                     PELRAIYSASLTQMVEGHTALTELSINKGWFKPYDKPIQQLTCSYKESQNVID"
     gene            complement(32209..32922)
                     /locus_tag="L3M60_000443"
     CDS             complement(32209..32922)
                     /locus_tag="L3M60_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBN6225146.1"
                     /translation="MINLIFDVDDTLYNQLTPFYIAYNKVFSSIKNISIEDLYMSSRK
                     YSDEVFHMTENGEMAIKEMHIYRIMKAFEELGTSITEKDAQSFQDEYIYQQSQITLIP
                     EVEWVLNFSKERNINLGIITNGPSTHQRMKLKQLNIENWVDKNNIFISSEVGFSKPDT
                     NIFRVAENVMNLDRENTYYMGDSYRNDVLGAKKAGWKSIWLNHRRHEVEGVFYKPDFL
                     ILEHKDLMSLFIKICSKKC"
     gene            complement(33010..34275)
                     /locus_tag="L3M60_000444"
     CDS             complement(33010..34275)
                     /locus_tag="L3M60_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBN6225147.1"
                     /translation="MKKLPIKTVVQRLLELQEEGKSATLLGIGPMSPNLLQASFELAK
                     DDDFPLMFIASRNQVDADELGGGYVNGWNQETFTKDIKKIADKVGFDGLYYLCRDHGG
                     PWQRDKERNDHLPVDEAMELGKKSYLADIEAGFDLLMIDPTKDPFEIGKVIPLDVVLE
                     RTVELIEYCENERKRLNLPEIGYEVGTEETNGGLTSTETYETFITRLKVELDKRGLPM
                     PTFIVGQTGTLTRKTEQVGTFNFRNAYDLAQMAKKYGVGLKEHNGDYLDDVTLLEHIP
                     SSIIATNVAPQYGTEETRAYLKLAEVECKLEKEGLVDKTSNIRHVLLVNAIECGRWRK
                     WVIGEQKNLTTEDIFKNEVLSNEILDIAGHYTFNNDDVKKEIEVLYANLSKNNIDGQR
                     FVIDHIKRPLRDYAECYNLKGVTTRILNK"
     gene            complement(34379..34687)
                     /locus_tag="L3M60_000445"
     CDS             complement(34379..34687)
                     /locus_tag="L3M60_000445"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBN6225148.1"
                     /translation="MKIVAVTSCPSGVAHTYMSAESLELSAKKFGIEIKVETQGSSGI
                     DNELSLKEIEEATCVILTNDVEIRNMERFKGKKVVRMSVSDIIKKSDALIKKIKDTFQ
                     "
     gene            complement(34758..35813)
                     /locus_tag="L3M60_000446"
     CDS             complement(34758..35813)
                     /locus_tag="L3M60_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBN6225149.1"
                     /translation="MRILKFLQKHLMTATSYMIPFVVAGGILFALSVTLSGQAAVPET
                     GWLAKLNQIGAAGLALFIPILGGYIAFSMADKPGLAPGMIGAYLAKEVNAGFIGGIIA
                     GFIAGFVVLQLKKIKLAPTMRTLGSIFIYPLIGTLITGGIIVFLIGEPIASFMTWMTN
                     WLNGMSGVSKIPLGGILGGMIASDMGGPINKVAATFAQTQVDTLPYLMGGVGVAICIP
                     PIGLGLATLLFPKKFSKEERDSGKASLLMGCVGITEGAIPFATSDPVRVIPCIMVGSI
                     IGNIMAFLLGCLNHAPWGGLIVLPVVDNRLGYIASVLTGAVVVAILMKLVKKDVKEDE
                     EIEEDLDDSIELIFEEL"
     gene            complement(35815..36267)
                     /locus_tag="L3M60_000447"
     CDS             complement(35815..36267)
                     /locus_tag="L3M60_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225150.1"
                     /translation="MSKLVNVKCSFKIHANSKEEAIISLVDVVSKEGYLRDKNQFLED
                     VLKREETLSTYIGHGIGLPHSQSVGVKNSCITIGKLDTPIEWTEEGEKVDLIFLISVT
                     KDNENNLHLKILSKLARLLMHESFRNQIRCSDEQTVYNLIKEKIEGED"
     gene            complement(36264..38294)
                     /locus_tag="L3M60_000448"
     CDS             complement(36264..38294)
                     /locus_tag="L3M60_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225151.1"
                     /translation="MISGRMLLIMNFLKGKNKTSYKDISKQLSIEERKVRYDINNLNI
                     VLRSLNKPVIQKMNKGVLIIPDNFQVVCINNNEYVFSLTERVSIMKIISMFKIENLNL
                     EKLSKEFQVSRSSIKNDLNVLSSELERNQITITYDKAFKISGEEDIIYKQRLNILKSY
                     SYLFGKEKSDLSVFEKYLVNVIEKIFKGISLIDIYNWSMNLLLQMGWTLNDESFTWYV
                     SNIFLFTWYIHSDEKHPLESVSLSEPCFENKFDQEFETIIQKKLTKEQIFLLKSFVFF
                     TNKYASLNEDMDLISTEIIVQDLIYNMSEILEISFKEDMILYKGLLNHIAPLIERIKQ
                     NIQIYEELLHVIPDKYQYVLEATKVSIQKNRVLNQIKNENETILLAIHFLGSIHRNER
                     NNYKNILLVCGFGYGVIAMLKDSLINDYQINIVDSIPSYKLQDYKDWESIDFIITTSK
                     IVIDLPKTIIEINPFLQEEDYLKFEEKGIKRKNVLTNYMSIKKRLDFLEEDAQKRVLS
                     VICQELGYSDERALSRQLNLGDLIGIDTIRIIDEDMKWEDAVKISSNILANCDFVSSQ
                     YAENIIDILKNVGFYAVKDNEFALLHGNDSSLVKVSSISLLICKEAVSFGDKKVRIIF
                     CLASKDKKEQIPAIINLTKMVYKTNFISMLESAKTKEEAEGLIHKYEKEVTV"
     gene            complement(38559..40787)
                     /locus_tag="L3M60_000449"
     CDS             complement(38559..40787)
                     /locus_tag="L3M60_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6225152.1"
                     /translation="MKLSRYKKNSLVLSIILCSLIISILVITVFNTVKLHNISSKQSK
                     NYLNDVSTQIVMNVDSKIKFILSDLRIMADFIKQYEGDSRYEYLSKRKTSYKYYDIGI
                     IDLEGKAEFLSGKKFDLKNTDSYKKGIEGKEYSEVIESMGFVFYSVPIFNDNHEVSSI
                     LIGVSNKESMDEILNIDNFNGEGTIEIINSQGRPLFIGKNTELIKDLGHKYKSSGDEP
                     WAQKMLNDFKNDKSGNITITSSKGVQCLLTYYPITKGLNDWHFVLIVPEDAVLGELNK
                     LNSFTIIMTFIITCIIGIITLILYIIRRKYVNQIENIAYLDSITNGINSTKFSILVKP
                     LILESPDSTYMMVAINIKDFKLINDCFGSKKGNLTLKHLYNVLYKNIDENEEFVCRHD
                     ADLFYLLIKNRPVLEALELLYKIEADINYFNKNRENPYFLRISAGIYTIENHNEDLIT
                     IQDHANTARKSFNKAHQSDFSFYSDIERKRLISEKEISNLMEKALENKEFFMCLQPKI
                     DIKSGKISGAESLVRWKNSEKGIIYPSDFIPLFEKSGFICKLDLYILEETCKLISKWI
                     KEGKEVLRISVNVSRQHLNDKLFLEKYKLICNKYNVPTYLIDLELTESIFLENPEAVD
                     IIDDIHSNGFKCSIDDFGFGYSSLGILKDFKVDIIKLDRSFFVSKNNIDRGKVVIKSI
                     IELSKRLGMKVTAEGIEELEQVEFLKNVGCDYIQGYVFSKPLLISDFEDFAYENNKIK
                     NI"
     gene            41436..44864
                     /gene="uvrA"
                     /locus_tag="L3M60_000450"
     CDS             41436..44864
                     /gene="uvrA"
                     /locus_tag="L3M60_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBN6225153.1"
                     /translation="MIINNYQNQSIITNPKKFENEYQYLPDTPIELLKVVQNLIIHGD
                     QGKLYGISFNKQQSDEELLRTVPQMLKRIFEINSNSLTIPRTPKQRLVGMCRDYSLLL
                     VSFLRYRGFEARMRAGFANYFESELTYEDHWLVEYYDTATKRWIKIDAQIDDIQKNYF
                     QINFDAHDVRRIDGFLTGSEAWIKCRQEHTHPDDFGYNKNWKGWHSVKGNLLHDFNNM
                     IGLELLPWDLWTELSSKKYNQLTRAEKNLLDEMAEILSSGNKKIEDLNLLIEKLPEDY
                     LKSIFSQLKILGISEIKELANPLELEKKFKFTKAINKPIKNSLCHNKSSIYLKGGRQN
                     NLKDVEVTIPKNKITVITGVSGSGKSSLAFDTIYEEGKRRYFENLGNGAKLSEQLQKP
                     EFDLLQGLTPTIAIEQKKGNQNPRSTVGTLTSIWDYLRMLFVSIGKSYCPYCKTPLEK
                     KNNTKNYCPHCQTIFSKINTSTFNANSHTGACHDCNGLGFTYQVNPQLIVKDPTISIL
                     DGATYYFGKLRGKKPNGNWMVGELYAIAKDKNIDLDIPWNELPRDFIDAILYGTDDKI
                     YEFSFESRGRESKIRRPASGAINHIQRLFRESSSENNTLHQYMNKIPCNTCGGELLCI
                     EARFTTIKGYRFPELTKMTIEQLWNWLCELTNQLQQKELSLVNDILTELKIRVSYLLK
                     VGLSYLSTDRTAPTLSGGELQRVRLSSQLGSELVGLTYILDEPSIGLHPRDHNLIIKM
                     IEELRDKGNTVIVVEHDKDTILSADYIIDVGPNAGIKGGFIIAEGTTQEIIKNPNSIT
                     GKYLSTYNKISSQNKIIPSKWLSLKGCHANNLKNIDVEIPLNCMCSITGVSGSGKSSL
                     VFHSLLPALEEKLKQKSIPNKNYTELTGFDAIDDFILMDQTAIGKSSRSTPATYINIF
                     DEIRSLFAETPQAKQKLLDESYFSFNSKKGQCPNCQGLGKTKIILQYIADQWVTCNKC
                     QGKRYKKEILSIQYKGKAIADILDMEVAEAKTFFSDCSDIYRKLYLLDEVGLGYLKLG
                     QNTLGLSGGESQRIKLAKELGTKTKKRMLYILDEPTTGLHFKDIENLLITFRKLVNEQ
                     HSLLIIEHNTEVIRASDWIIDIGPDSGMNGGEIVASGTPDEIKVNPNSITGHFI"
     gene            45049..45882
                     /locus_tag="L3M60_000451"
     CDS             45049..45882
                     /locus_tag="L3M60_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6225154.1"
                     /translation="MSWKHIKIPNGDTMIFCNSFITSKLSMNIIVTHTPIVTTLEMQK
                     AYEPFTKYNVNIFAFDFSGTGKSDGYEKDFSRNSIIKDLDSIITYIEKNYSSNIHLYG
                     NTGIGGMFAQYYACSDNKLKSFAQFACVDYKNTTGVGYPYLTVKLLNLFFKFLPNFHI
                     TVKPPKYKGYNEVYDNEFYKIFEEKNPHIWKNSTKIINTMLECFIAEDSKIKDGVNIP
                     TLVFKTLHDRYFKTQYFDSYYNSLKCKKKLVEINNVHNSYYIDSEKFCQEAYDWFVEN
                     Q"
     gene            complement(46011..46577)
                     /locus_tag="L3M60_000452"
     CDS             complement(46011..46577)
                     /locus_tag="L3M60_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBN6225155.1"
                     /translation="MNTIWNLFFIFLKIGLFSIGGGYAIIPLIQEKVVNQTGWISEKM
                     FTDIITISQMTPGPLAVNTSTFVGLQIGGFWGAVAATLGCVLCGVVISLFLYNFFQKH
                     QNSTYIFEILNGLKSASLGLIISAAATIIILALFGTNKVYFNSIFEMLNWTALIIFFV
                     TLLIVRKWKINPIVIIIISGILGIFFYL"
     gene            complement(46574..47137)
                     /locus_tag="L3M60_000453"
     CDS             complement(46574..47137)
                     /locus_tag="L3M60_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HBN6225156.1"
                     /translation="MNQSRKTYFWLLIINLFISTFTFGGGYVVVPMIRRYFVNQKHFF
                     TEDDLMSMAAVAQSTPGAIAINLSALAGYRVAGTLGVVISCIAAIIPPLVILGLVSAF
                     YTTFISNAIVTAVLKGMQAGVAALIVDLIIDMCSMILKQRSLFLSAMIPIAFFANFVM
                     SINVIFILSVCCCVCVMQVFYKKRKNK"
     gene            47257..48132
                     /locus_tag="L3M60_000454"
     CDS             47257..48132
                     /locus_tag="L3M60_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6225157.1"
                     /translation="MINIRYLEILEAIEQTGTFTGAAKKLHITQSAVSHAIAELEQQA
                     ETSLFDRLPRGVCLTRCGISLLEEARSILTACRNLDKRISRLEECTPINIVSSITIAS
                     FLLPPILSRLKNLFPSLKVNVRVESANSAMDILKSGEADICFWEGIEPQGAFRTILLG
                     SYKLCVACAPTFPLSEQTLSLRQLCTFPLLLREQGSAIRDTLDSVLSLSNQKAYPIWE
                     SVNSFALIKAAEAGLGITILPENLLLDSLLHKKLRLIELDGIDMENKMLAILHKDKNI
                     TQPLKIILDNISNVL"
     gene            complement(48280..>49466)
                     /locus_tag="L3M60_000455"
     CDS             complement(48280..>49466)
                     /locus_tag="L3M60_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBN6225158.1"
                     /translation="GEVSGSSMDSYFSARNKDIDSNTERYIETPISKDMKEVGYDVAV
                     LGNHEFISNNKFYLDNMISDFEKQKINILSANTYKKNGDSYTKPYIIKNINTPEGNVK
                     LGILGLTIKEVGERKQWKDGKLVDSKSLELKDQEGYNGELYMNDLVEDAKKWVSIMEK
                     EKPDIIVAVAHTGEKPKKPRNPGNRIQDLAQQVDGIDAIVAGHNHVQIEQHDYKNKSG
                     ENVIVTEPGKHGECISKINFKLEKTNDKWNIVDKSSKLIKFEKNQESDKFGEFISSIN
                     KVSKDKKEIRLSEVKTPEWDKIYVFKSGTSADDIYKTIGYKWRNISSPKGDDMVQIVV
                     MNNNKPVSYVYGEAEVMPINIKCDESEYKDNVVTIYPNKNDKFKIEKGKQEYSINLKH
                     IQ"
BASE COUNT        15962 a         8756 c         5358 g        19390 t
ORIGIN      
        1 ccttacaaaa tattcttgat gtatttttta aatatacttt attgaaactt tgttcttgta
       61 aatatatttc taacttattt gtaatagcat ttacatgcct aatatcatta aaaccacttc
      121 ttattataat acctccaaaa gtgcttccta aatgagaaaa aaatattctt tcaccatttt
      181 cataaatttc acatgcaggt acgactgcta gtatttcttc acctttatat attattaatg
      241 agtggtcagt aaattttccc tcttcatgat aattaaggaa atttctagtt tgcagaaatg
      301 taccatttat agatttattt tctacaaaat aatcccagtg tttttcacag cttttttcaa
      361 atttattgat acgaatattg ttcattttac cacctatgac atgtttttat tttttaccat
      421 ataaccatat aatgctacaa aaagctgata ttcttcgatt tcatcatttt tatataaatc
      481 acacttaact ttatgatggt tagcttcatt atatattttg tctatatagt tttttatttc
      541 aatacagtgt ttgtctataa caaaatgttt tattcctgat tgtttttctt ttatcttatt
      601 cctgtcaaat tcactagtat tgtacctttt tatcatgtct ataacctctt ttatattctt
      661 ataatcaaac ctatctataa aattccacat attcgtaaaa attggtgaaa cttcattttt
      721 aaacttaaca ggaattaaat catataaaat tgcatctaat tttgaagtgt gacttcctac
      781 catgaaagac tctagatata tatccgaaat cttataaaaa tcgtctatat ttgttgtcgt
      841 cccaaaacat ataactctat tatctgtaac gttacttaga gttttccaat agatattctc
      901 tgtatcaact cctacaacaa taataattgt attatccgta ttttcaatca atgttttagc
      961 aattttaaag taattaatat catttacact attgtatttt ctttcactag caatagtcaa
     1021 tattattgtc acatcatcct ccaaattata ttgttttcta atttcagatt ttgaaaatac
     1081 ttctttttct tttaatagaa ttggtaatat ttcagattta tctatacctc ttttattcaa
     1141 tgtaattagt tgactagctt cactaaagtc aataacaata tccgcaatac ttgcccctaa
     1201 ccaaaaagtg ctgtcataat gattaacatg taaaataggt attttcccat caactccaaa
     1261 agcaacaaca ggtattgtat cccacatatg aacatataaa ataactatat cagcccagtc
     1321 ataagctatt tttctcaaag ccatagctct ttttaaataa tcatctgaaa ttaaatctaa
     1381 agatataatt tcgccaccac tatcatctat tgcttttaat aaccattctg ggagtgtgtc
     1441 tgattgccat gtagttacaa ctgaatgaac agagtctttg tcaatctcaa tccagttttt
     1501 aactagtctt gtatgacctc ctgttgaata tccttcactt aaaacatgta atacttttct
     1561 tctttttaaa tctttttttg taaataattt cgtattttct atttctggta aattatttgc
     1621 acattctatt aatgctctct cgcatttttc acaagaatac agtccagtat gattaaaaac
     1681 agaaaaacga gcaattttag aaacaatatc taaaacatcc ttataattct ttaatattaa
     1741 ttgttctgta agttcctctt tcatacaatc cattacatgc ttattactct taagtatggt
     1801 acttgctttc ttctcatcaa attcataaaa attctctcta tctaaatcaa gatttatgta
     1861 ctcttttaca aatcttatcc tatattcatc atagccatta tttatatttg ctactccaga
     1921 atcatgcata caccaaggct tattctgttt tggtataata actttgtatt tattttgtct
     1981 aaactccata ctctgtgaca aatcataaaa atgccatcca tcaaacttat cctctctcca
     2041 gaatacatca tattgagtaa tcataatcaa tccatctata gccataactt cattaaaaca
     2101 ttcatcaaag ttactaaatt cataaatatc catcttacct gtatgactat catatacttt
     2161 ccctactttt ttatcagaat cccaccaaat tccattcaca ggtatatcct ttactccagc
     2221 tacaccaatc attcctacat ctgcattttt tttaaatatc tctaaaatat catatatgaa
     2281 atttttatta attataaaag tatcttgatg taaatatacc ttatacttag aatctgtttc
     2341 tctcatagct ttattataag cagaggcaat agaatcggct cctcttaaag ctactgtttc
     2401 tatactaaag tttttaggaa tatctaggct atttatatat acaagacatt cttcataaag
     2461 tctttcatca tttacacagg ttataaaaca tatcttatca ttattcatat ctcttcccct
     2521 caataaaata aagtatctct tgtatgtcac tttcactctg tactttttcg ctactatttt
     2581 taattaaatt ataagcaata tcaaattctt ctaattcaca taatacactt gttatattat
     2641 atattgagtc aatatcatct ctatccatct ctaatacttt cataaacatg tttatactaa
     2701 aatctcctag acctctgtta aatgcctcaa ctcctaatct gtttaataga gttactttat
     2761 ttatcacaaa tgtatcaact aaatattcaa tatcatcaac aaaacatggt tcagttatat
     2821 tataattatc aaatatataa cttaaattac catctatatc caattcatta tcaattctca
     2881 ttaaagcgta ttttaaatta accatactaa tactagaata ttttttggta tctatgcttt
     2941 tagtggcttt cactatatat tgataagatt catactgttg tctcatttct ttacagcttt
     3001 ccttacacat tgtatcaata aatctattat cgatttccga aagcggcata ctaatagctg
     3061 taatactatt tattatgtaa ttactatcat taaacatttt cgttatctca tttaatgtaa
     3121 aaaaccttaa atgggtcttg tccagaatcc ctgaatcttc atatgtaaaa ttacctaaca
     3181 caagatttct taaaacagaa acatgcataa catttggtat actagatacc aaataaccat
     3241 ccttttttag atattgtttc aatttattga ctatcttcca tgggtcaatt aaatgttcaa
     3301 gtacatctcc caatattata taatcaaaaa aatttttttt ataatccaat tttacttttt
     3361 ctatatctgc tattagcacg tctgcactat attttgcaag tttaccagct gactcactaa
     3421 tttctattcc atatattgat gcatttttat atatattttt tatttctaaa agagtagctc
     3481 ctataccaca tccaatttct aaaacattga tatttgtctc tggactatca tctattttag
     3541 aaatcaaatc tgttcttata attccactat attctggatt aaatccccac ttagtctcaa
     3601 attttcttct attaatgtcc attatatctg tatatttatt gcgattttct ttaaaagata
     3661 tacttccaaa atggtgtata aaagtgtctt tgcataataa taacttatat cctgactcta
     3721 tcattctaaa agataaatca tcatcttcaa aatttccagg cgtgaatagt tcatctaaaa
     3781 ctcctacatt atctattaca gattttttta ttaacataca aaaaccaact aacttcaatc
     3841 tatatttcca tagattaata ttagatttat ttattttttg tgcaaatata aacatctctt
     3901 caactgattt gtagtcaaca tcaatctgtt gatagtatga acatgcattt gttattggac
     3961 ctacagctcc tatattatct tgtgaccata aagccttatt caaattataa agccaatttg
     4021 gagtaactat tacatcatta tttaacaaca atatattttc accattagcc aactcaatac
     4081 cttgattgca accttttgga aatcctaagt tcttgctatt atacacagat tttatatcag
     4141 attgtccctt taaccactca actgttccgt cagaagagtt attgtcaatt attattattt
     4201 catatttatc ctttggggta aatttcctaa tactttcaat gcataactta gtatattcta
     4261 tttgattgta tgtcaatatt attatgcttg taaattttat atcttcattt gtatatgtat
     4321 attgccaatc ttttattata tctttagatt tttcattaac caatattggt gtattcattc
     4381 ttcaattctc ccatttttga cttatcttaa actttttaaa tattctatat tatatatagt
     4441 gtcttcattt tctacatcta atgataatgc cttttttaac ataaagtcag ctttgtcaag
     4501 cttattttca aacatatata aaattccctt tatgtttaaa atctttacat catccacaaa
     4561 tgtttttgag tagtcatcta ccaataattt cgcatcattt attttgccag tttcaatcat
     4621 attttctata ctaataataa aactatcttt taaatcccta aactcttcac tttcttttat
     4681 attttcttct aattcaccta taagtatttt tattatttta ttataaaaag gatactcatt
     4741 tagtaatttc ctcatactat gtatatactc taatttttca ttagcactat ttactttatt
     4801 tttaatgtta agtagatgct taaattctat agtgaactta tctaaatcat tacaaacaaa
     4861 ttttattaat tcaaagtcac ttaaattgct atatatgtat cttatgtact tataagaata
     4921 cattatataa agataaaaaa tttccttata tttttcatca cttaagctta tactttctaa
     4981 aactacttta gatatagctt tatatactct tatcttttcc atatctgatg tatttggaca
     5041 actaattatg tacttatata gcgctaaatt aaaacttctt tctaatgcta aaagatactt
     5101 tagatatttc tctatccaga catattccat ttcataaaaa atatctagta aatcaagctt
     5161 ctcttcaaaa gctattttta taatatatgc ataaattatt tccttttctt cattgagtat
     5221 ttctttacat ttatctatag atacacattt atttatcctt atctgattta aaatgccata
     5281 attaccttca atgtcacaaa gaattttata tacatactct ttttcatctt ctttaatatt
     5341 atttataact tcttctatat ttctataaaa actatttttt tcaatttttg aatttgaaac
     5401 ttctttataa tagtttttta tatcttcaaa tctattcaat ttatataggg acataaataa
     5461 actaaagtac acattctttt tcttttctat atcttcaatg ttattatact ctgaaattac
     5521 agactcatat ctttcaagct tataataaat ctttataagt gtaataatag cctcatccct
     5581 aagccctaca gtatcagtgt ctgaaaacaa actatttgct tgtgtactta tttcataatt
     5641 atctaacaaa taaatgtatc tcttatacga atctatacta ttttcatact tccctaaatc
     5701 tacttgtgct tgtgcaatat aataatatat atcaatatta ttcatgtctt tcttaatata
     5761 ctttagacac aatttttcac acttcttatg ttttcctaaa tataaatatg tttttacaag
     5821 gcttgttatc aagtaaccag gtgccgattc tagtttagaa tataattcat aagacttctc
     5881 aagataatat aaagcctcct gatattttcc taatataata aaattttttc ctaattgata
     5941 atttgtatat ggagaattat cattctcttt taactcttca aataatagtt tttcatttct
     6001 ttttacttta tttaatctta tttcttcatc ttcaaacaaa tatccataat gttcaaagtc
     6061 agcaacatta ttaaatatag ggtttttgta tattggttgt tcatgtactt taccatggta
     6121 tctaaaatca acgtttttaa aaagtctaag cactgaacaa tatccatata attttttatc
     6181 atatgagtaa agatttttta attgtattga agcagaatta aattttttat caagaccatc
     6241 tttaaaaaat ttcacaatag ttgaagaatc aatcagtatt tcgtctgcat ctaatataag
     6301 taaccattcg cccttggcat aggatattga tatatttctc atactagaaa aatcattatt
     6361 ccaattatga aaatacactt tttctgtgta ctttttagat atttttatag tatcatctgt
     6421 agaacctgta tcaactatta taagttcact ctctatagaa tctcttaatg tggttaatga
     6481 ttttaaagtt ttttcaagta ttttttcttc atttttaaca atcataacta tactaagcaa
     6541 catatatatt ttcctttcaa aagtatattt ttaaaaaaag aaaggagtaa agcaaagcct
     6601 taccctttct tctatatcca atatacaaaa ttattttatc ctaataattg taaaactcct
     6661 tgtggttgct gattagcttg agcaagcatt gactgtgatg cttgaactaa tatattcatt
     6721 ttagataagt taaccatttc tgaagcaaca tctgtatctc ttattcttga ttcagctgct
     6781 gtaacatttt ctatagtatt atttaagttg ttttgagttg attctagtct attttgttgt
     6841 gcccctaatt ttgctcttga tgtatttaat tctttaagag ctacatctaa actagatacc
     6901 atttgtttgg ctgcatctgt tccagaagct ccatctacat ttaagcttgc tattgtagaa
     6961 cttgttatac ctgccttact cattatgctt gaagtattta ctagcgcaac ttttatttca
     7021 ttgttgttgt tagttgttcc tgcaacattt gttccaaaat tagctccaac ttgtaatctt
     7081 atttctgtag aacttccatc aagtaatttc ttaccattaa actcaattga gcttgatatt
     7141 ctttcaactt catcctttaa ttgtaacaac tcatcagcta tcttttgtct ctcttcagca
     7201 gtattagttt catttgaaga ttgtactgat aaagttctca ttctttgtag tatattacca
     7261 gtttcttcta atgcaccttc tgctgtttgt acaacagata taccatcttg aacgtttctt
     7321 cctgcttggt ctaaaccttt tatttgagct ctcattttct ctgatatagc aagccccgca
     7381 gcatcgtccg cagctctctt aattctaaca ccagaagaca gcttctccat agacttactt
     7441 tgtgcactta cgtttcttcc catttggtta tttgctatca aagcacttac atttgtatta
     7501 actctcatta ttttaccctc cttagtatag ttgacatcct tgtcatttct actcttaact
     7561 tttttattaa ttcttcattt atataatcgg acaagactat atataacttt atactttttt
     7621 attaaaaata tttacttttt ctctatttaa gctataatac attgtgttca cttgtttagt
     7681 aagcttatac tcatgtaatt ctttttttac ttctaataat tgtccttgta aaatatctct
     7741 tattctacta tcaatttcaa ttaattccat tttattataa agctgtataa aatcatttct
     7801 atcaatttca cttacactgt ttataatctc ttgtctttca tctaatttgc tacttatctt
     7861 tatatattct tccttttcaa tcaaatttat aatttgtaaa gagatatctt tatataaatt
     7921 taatttttct tttaatttat ccataattaa ttaccttgtg cttgtgagaa ataattcatt
     7981 tgggaagaat actgattcaa caaagattct aaagctgaat atttagcata aagtcttttc
     8041 tctttatcat tcattttttc aactaaccta cttatatttc tttcttgctc tgcaatttgt
     8101 tttgaataaa cattttcact agcagaagca gttttttcta tacctgcttt tttaggaagt
     8161 ttagaagttg aacttccaga atattcatat acaatatctt ttagcttttc aaacattccc
     8221 ttatcatcat aattcgaaga atagcctgct agtgatttgt ataccttatc actatcatct
     8281 ataagagcct ttgaaaaagt atctgcatta aacgatattt gacccttttt attgtaattt
     8341 tcgctttcat tgatacctag ttttcttaat gcaataatat tttttccatc tccaaaaaat
     8401 gcagattgaa tatcttcaac aaaacctctt agctcatcat catttctaag tataccttct
     8461 ttagctttct tttcccattt ttctatttct tcagttgtca tatcttcttt ttgggcatct
     8521 gtaagagggg gataatcctt tggtttttta gtagtaacta aaccatatac cttgtccata
     8581 agtttattat aatcatccac aaatgacttc atatttttaa cagcttcttc agtatcagtt
     8641 acagaagtta actttgcagt tccttttgaa tttacattat agtcaatatt atctatatta
     8701 aacgaattct gttctttact tatatgagtt actcttccat gttcatcagt tacatcagct
     8761 tctagatttt ttccatatac acttgccaat ttgtttccac cagcatcact tacaaatgtg
     8821 ataccattat cattagcaat ttgctgagct aaattgtcat ttccaattac tcttaaatta
     8881 atatctgatg atgaaccagt ctgttttctt gaaataatca attcacctgt catctcacta
     8941 taagaagcct ttatatcatt atctgcacaa agcgagttta tcttacttac aatagtgctt
     9001 gtagtatctt gttcagagat tggtatattt acatctccta tagttaaaga actagctcca
     9061 ctagcatttg gaggaaactg ttgaacaata ggttcacttg aatttaattt gatagtaaat
     9121 tttgctggtt cagccatttg agaaactgca aattgataat ttattttttc tgcaccagct
     9181 gagccttttg ctactataat actttcatca gaactagtta tttttgtaga agagtatgct
     9241 ttttgactta ctatactatt aggagaatct acacttagat atttatcata aagatctttt
     9301 gattcttgta taatatttct atatatttct tgcttccatt taacgatttg ttgctcttgt
     9361 tttgctttat caactttttc cttatctcta atcatactag cttctattat gccttccata
     9421 tcaaaatttc ctgaaagacc tgtaactctt ataggactta tacttgacat ccctctatcc
     9481 ctccatccaa ttatattttt attttattgt aaacttcatg ccatgtatct ctaatttgtt
     9541 ttactattgg caatatatca tcaattattt taatatcttt attttcatca gctattgcta
     9601 attcagattt tataaattca taaactttat acatatcctt tgcccattgc cctgcatctt
     9661 ggtctaatgt agtcattagt tctgtaaata tattttgaac tcttacaagt tcattatgtg
     9721 cttttaaata atcgttattt aaaagagcat ctttggatat gttagtatat ttgactgtag
     9781 catctacaag catcaatagt aattcttctt ttgataactt acctaattca gctaaattta
     9841 attccattat ttatacctac ttctttcctc tttacttttt ttatcagctt catgccatgt
     9901 atctttaatt tgttctatta caggcaacac attatctatt atctgaatgt cttttctaat
     9961 attagctctt gaaagttcat atcttacaaa atcataaact ttatacatat cctttgccca
    10021 ttgccctgca ttttggtcta atgtaaccat tagttctgta aatatatcct gaactcttac
    10081 aagctctcta tgtgctctct gcgtattttt atctaagata gctgctctgg ctattttggt
    10141 atattttact gctccatcaa caagcataag caataattgt tcctttgatg ccataaaaac
    10201 agcattttgt ttataagaat tataaggatt ttctccatac atatattact tcctccaagc
    10261 ttattatttt aatgacttta aaatatttat atttttattt gtagctttaa tattctcact
    10321 ctttactttt tcgtatattt cttttcttaa tatgcttata ttgttaggtg ctgatatagc
    10381 tagtttcaca ttgtttccat ctacccctac aatttttact tctatgtttt ctcctataag
    10441 tactgcttca tctttttttc ttgaaattac tagcatccac tatcacctct caataatgga
    10501 tgccttattg aatatttatc atcttgtaaa attatttgca ttccaaaatt agtttttata
    10561 tttatcacta taggagcttt catgtttaca gttgattctt caaacgtctg accaagagta
    10621 attatattaa atagcataac atcctttgga gattttactt ctagtttttc tatggtttct
    10681 tgatttaaaa ctatttcata ttcattaata atctcaaatg gcgatacaac aacaaatcct
    10741 ataccttctt cttcagtaga attaaattct tttaaagaat cattatcttc tatatctaaa
    10801 agctcatatt cttttaaatt ttcaaaccct aatatacctt tttttaatgt aaccttcatc
    10861 atatctgcgt tatttttact atatataatc taatatagat acactaaata actttcctcc
    10921 tgtttgtagt gaagcttgat aagttaactc tgctgccttt aattgaacaa gcttttcacc
    10981 atagtctatt tcttcatttt gtgacagaat ctcctttaac tgtatcaaac tatcatcatt
    11041 tttttccttc atagcctcta ctgtatttgc ttttgtccca tatatagacc taacatttac
    11101 tgtattttct attagttgtt ccatttcacc taaacaagtt gttgtgagct cttttatagc
    11161 atcagcatct ggatttgcta atttctttga tatattattt aactcttcaa acgggtcagt
    11221 ctttgttggg tcaaatccaa ggctactcaa tttaacatta tagtctacac ttattccttg
    11281 agctacgtcc atacttaaag aatcttttaa tttattaaga tctatactag aattaaactt
    11341 taagcttact gagccatcag cattatctac acactcaaca ggtggtatac ctgtatttga
    11401 acctgaaaat atgtacttac cttcatgtgt tgcatttaaa gcatttgcaa tttcttttat
    11461 tttttcattg gtttcattac taagggtctt tagttcgtca tctgaatatg caccatcacc
    11521 acttgccacc aaagttttct ttatgtccga taataatttc ccaagctgat ctaatgcagt
    11581 atcagtagtt tctaaccaat ttcctgtatc tttacaattc tcaacataaa tctccatagt
    11641 ttttatttca ctttttgagt ttaatatttt tattgcttta tatggattat ccgatagctt
    11701 gtttatttct ttggttgtat taacttgtct attaactttg tccaatcttt gtagattatc
    11761 ttgcagggag tttagataac tgctagacat catacctgta ctaactctca ttataaattc
    11821 ctccttctat taaattattc catttattac aacatccaac agttgcacag ctgttgacat
    11881 aacttttgca ctagcttgat aggaacgttg tatttttata agctctgtca tttcttcatc
    11941 caaagataca cctgaaacac tcattcttga attatcaata ttatttataa ttttacgttg
    12001 attcacttca tcttgattca cttttttaga atctaaacct actaattctg caaaggcttt
    12061 atagtggtca cttattgtac ttacttttcc accagccact ccaatatcta ttttttcatc
    12121 ctttaactta gcaatttttg aagcctcatc agcagtaagc tttaattcat ttgggttact
    12181 ctttatagct ggatttacct ttatgatatc ttcaccatct acatttgaag ttacaaaaaa
    12241 atctcttact gttattccag cagacgtatt atagacatca tttatctgtt tagctaatcc
    12301 atcagagacg tcttttaaat cgcttttata tgtatcaatc ttaccatgca tttcaaataa
    12361 tccttgtaag gttcctgata cacctgtttt ccaagttcca tctgcatttt ttaattcatt
    12421 aagttctaat ttaccatctg caagcatatt tttaacatcg ctattttcaa aatctagctt
    12481 aaagctcaag ttgtctaata ttctatctct ttcatcaagc aaatcatttg gcatagagcc
    12541 agaaccagag ataatatcaa tagatttatt caaatcttca agacttttta acatttcatt
    12601 gatttgtttt atctcagtat ctatattaga atctatatct ccttttaact tgtcaagctt
    12661 cttataactt tcgcttatta aggttgctaa attagatgca ttttctacta ctagactttt
    12721 tttactgtca ctatttatat cctttgataa cgactcccat cctttaaaaa aatcatctat
    12781 acctgcagat attccatagt ctgatggttc atttagtata gtttctatag tattataata
    12841 atcatatttt gaaactgtct taccatactt atgagcttct gctctaaatt ggtaatcata
    12901 aaaactattt ctagctcttg ttacatctgt tacatgtact ccagtaccta attgtcctct
    12961 tccaattgaa ttactcaaac taggcatata catagggcta tttgtagcaa actcagtcct
    13021 ttgcctagaa taaccttctg tgtctaggtt acttatatta tgagaaattg tctgtattgc
    13081 tttttgatgt gagttcattc ctgaattagc tgtatttaaa atggaaaata atcctgccat
    13141 atttctcctc ctaatattta ttttctaata tttccataag aattataagt tgttgtttgc
    13201 ttatcaactg gttttatgaa attaatcatt ttttttgtaa aaataagttc tttctttatc
    13261 agagaatcat ttgattcttg ttgattttta gctaaagcaa gaatgtgttt taggtctgta
    13321 tatatttctt ttaaatatcc atctttacta ttttcaacaa cctcactcat tgaaatatca
    13381 tcaccaaaaa gctgtcttct ctttatttct atggatgcaa gttccctact aatatcttct
    13441 agtttaggat ttaacatatt taaattttct aagtcttttt caactatata atcaaattgt
    13501 tcttttaata attccatcat cttactaatt atattttttt cttcatacat tataactttt
    13561 aagtctgtaa tcatgacttt atcctcgcat atcctctatc attttttttg caatttcttt
    13621 tggttttatt ttgtaagttc cattttctaa tgcatttttt atttcatcaa cttttttatt
    13681 cctaatactc tcatcactca tatttgatac acttgccatt tcctttgcaa acttagatag
    13741 ttctatctta tctgttttat taattttttt aacatcttcc acttttttta caccagatgt
    13801 attctttaaa taattattca ctacaatatt tgaacttaca tttttaatat ccatagtttc
    13861 acctccaaaa ttacatactt ataatttaat tatcgctcag aaaataaaaa catttatata
    13921 attcacacat attttaaact ttttataaaa atatcccctt agctaaactc atacagagta
    13981 gcctaaaggg atactattta aaatgctaat ttatatctga atcttctatt ttagataatc
    14041 ttacagataa cttctcattt gtaataatac tttctccata agcaaacaat ctatcatttg
    14101 catatatatc taatttttca tccacattct tatttagttt tattatagag ccttctttta
    14161 aatctaatat attttttatt gtttctgttg tatttcctat agaaacagct acatttactt
    14221 ttgcattaaa aacattttga ttttcttcta taataccaga ttgttcttct attagttgcc
    14281 caaaattcaa ttcatatata tcctcttttt tactattcat aaaacttttc tccttgtata
    14341 atcttatcta tacatatcta tcaacaaacc ttgtatcata tcaacagtag aaacaatgtc
    14401 tatgttttct ttttcttcac taattaactt actagtcata tcctcaagtt tactgtcaac
    14461 aatctttaca ttcttataat actgatgttc ccaaaaacta ctattcttat caagtgaata
    14521 catattatct acaacagatt ttaggtattc ttttactgca tttttatata tactaatatc
    14581 gccatagttt tgagtaagta caagcctttc tcctgccttt tttacactct ctaaatttcg
    14641 ttttatatct tctagtgaag aagattgttt taaagagttt aatctatcag aaaatttatt
    14701 attttctttt agttttttga cttctatatt tatacttgga cttattttta gcttatctat
    14761 atttataccc ataataaagc ctaccctcta taatatttca taaccttagg aatataattt
    14821 tgtgtttctt ttggcatttt atacaggtca gaaattgatt ccactcctct actcttcatc
    14881 cttccaggac caccattata tgccatcaac cccatttcta tacttcctcc aaacatatca
    14941 atatattcct taatatgtct agttccagca tctatattct gctctatatt aaaagggtct
    15001 gtaacaccca aatctctaca attttctggc ataagttgca taagaccctt agcaccagca
    15061 gaagaaacag tatttggatt aaagtccgat tcaactttaa ttattgcttt tataagattc
    15121 gcatctacat tatatttttt tgatgcttgt tctacagcat tttcaattct caactttaca
    15181 tttttatctt ttgtatcaac atctataaat ctactagtgg tattatttac tacattctca
    15241 agttcattaa acaaattctc agaattagac gatatattat agccattaga agtatgttga
    15301 ttattttgtg ataatgcttt taacaaggtc atcataatca tatcaaatcc agatgaatta
    15361 ttatatgtac aattacattg atttgtatta ttactaccag ataattgagt caatgctaat
    15421 atattaatta gtgaacttat ctcattcaat ataaaacccc ccttctaaat tttataaatt
    15481 tcaaagttac actgataaaa cagatttcca ccaagattca ttttctatgt accacttaac
    15541 agtttctttt ataccaaaat caaaatcata ttttgccttc caacctaact cattctgtat
    15601 tttagatgca tcaatagcat atcttaagtc atgaccaggt ctatcattta cataggatat
    15661 taaatcctca gatttaccta aaagatttaa tataattttt actactttta gattacttct
    15721 ttcattatga cctcctatat tgtaaatctc ccctatattc cctttatgta ttattaagtc
    15781 tatagcagta caatggtcat atacatgtaa ccagtctctt acattttccc cattaccata
    15841 tactggtaat tgtttatttt ccaaagcatt taaaatcata agaggtatta atttttcagg
    15901 aaaatgatat ggtccataat tgttggaaca tctagatata gttgtcaaca atccatatgt
    15961 tctataataa gaacttacca ataaatctgc tgatgcttta gaggctgaat atggagagct
    16021 tggatttata ggtgattgtt ctgtaaagaa taaatccttt ctatcaattg gcaaatcacc
    16081 atacacttca tctgtagaaa tctgatgata ccttttaacc ccatatttta tagatgcatc
    16141 taaaagtact tgtgttccta taatgtttgt ttttataaat aaatcaggat tctcgactga
    16201 cctatctaca tgagattcag cggcaaaatt aacaactata tcaaaccttt cttcttcaaa
    16261 tagcttatac atctgttccc tatttgaaat atctcctcta acaaatttat aattggaatt
    16321 gtcttgtatt tctattaatg tctctaaatt accagcataa gtcaacaagt ctagattaac
    16381 aataaaataa tctacatact tattaagcat ataatgtaca aaattgctcc caataaatcc
    16441 tgctcctcca gttatcagta tctttttcat attttttctc ctaaatgtta attctaataa
    16501 atttttatta acgtcttata cagatatctt taaaactttt ttgtttttta tccttttctg
    16561 ataaaaatat ttcttcaatt ccttttaaag gccactcaat agatatttgc ttatcattcc
    16621 atataaaacc attctcaaat tctggataat aatagtttgt acatttgtaa ttaaatactg
    16681 ctttttctga taaaactaaa aatccatgtg caaacccttc tggaatataa aattgttttt
    16741 tattcttatc agacagtatt acaccttccc attctccaaa tgtttttgaa tcttttctta
    16801 aatcaactgc aacatcgaaa acttctccct ctgtaactct gactaatttt ccttgaggat
    16861 atctaacctg aaaatgcatt cctcttaaaa ccccttttct tgaactagac tcattatctt
    16921 gaataaagca catatcaaga ccttgctcta taaaatccct tttattatag ctttccataa
    16981 agtatcctct ttcatcacaa aatacacttg gttcgactat atacaatcct tctattttag
    17041 tctttatgac attaattttt cccattataa caaactccct taataaaatt tataataaat
    17101 tttatctacc atattacata tcttcactag ctaactttaa tagatattga ccatattcag
    17161 tttttatcag tggcttggct agttccatta attgtgctct acttatatat ccttttttat
    17221 atgcaatctc ttctaaacat gctatatata atccctgtct tgtttgaacc acttcaacat
    17281 agttagaagc atccaataga cctttatgtg taccagtatc aagccaagcc atacctcttc
    17341 ctagaagttc tacttttaat tttcctcgtt ttagatactc actgtttata gaggttattt
    17401 ctaattcacc tctatctgat ggttcaacta gctttgctat ctctacaaca tcattatcat
    17461 aaaaatacaa tcctggtata gcataattag atttaggaac tgtaggtttt tcttctattg
    17521 acaatacatt aaaatcctta tcaaattcta caactccaaa cgaatttgga ttacttacat
    17581 gatacccaaa tatagttgct ccttttcttt taactgcttt ctccaatctt tcactaaatc
    17641 catatccata aaatatatta tcccctaata caagagacac tctgtcattt cctataaatt
    17701 cttctcctat tataaatgct tctgcaattc catttgcttt ctcctgaatt gcatatgtta
    17761 ttttaagacc taatgaactt ccatctccaa ataattcctc aaatgctttt atatctcttg
    17821 gtgtagatat tattaatata tcttgtatcc ctgccaacat aagaaccgac atagggtaat
    17881 atatcattgg tttatcatat attggaagtg tttgttttga tatacatttt gttataggat
    17941 atagtcttgt accagaccct cctgctaaaa taattccttt catctttttc catccttaaa
    18001 tttgtttttg tcatattatg attatcggat aaataaaaga aatatttata ttttttataa
    18061 tatcttattt tcttttctaa aaataataca tttactcttc attcatatca tatctttcta
    18121 aatattcttc aatggattct tcccaatttc taaaagaatt taaaccaatt aacttcaaca
    18181 taaaattatc caatacagaa tataatggtc tttgagcctt acatttaaat tcgttactct
    18241 taatcggatt tacttttatg tctatatttt ttaattcaaa tatctttttg gcaaaatcat
    18301 accaactaca ctcaccttca catgtagcat gataaactcc ataatgttct gtttccatta
    18361 ttcttattat aatttttgtt aaatctactg tgcttgttgg agttccaaac tggtcattaa
    18421 ctacattaac ctctttattt tctaaagaaa gttttatcat agtctttaca aaattattcc
    18481 cattaccata taaccatgct gttctcaata taaagtactt atgtgaaaat tgttcaacaa
    18541 acttttctcc cataagttta ctttttccat acacagaatt gggttcagtt ttactgtctt
    18601 ccctatatgg atatttagaa aaaccattga atacataatc agttgatatg tgaataagtt
    18661 tagcattaac tttttctgat gctattgcta gatttcttac tcctaaggca tttactttat
    18721 atgcagtttc tatattatct tcacatgcat caacctttgt atatgctgca cagtttacaa
    18781 ctacatcagg tttattattc aaaataaaat tatctacatt agtctgatta tatatatcaa
    18841 aatcattcct tgttgtagct aatatagcat atttgggttg actcatactt tgattgattg
    18901 cttctaattg gttaaccaat tcttttccta attgaccatt agaccctgtt attaatattt
    18961 tcattaatta tgttatcctc caataaatgt tcaattttac ttaataatta attataacac
    19021 agcttgcatt ttaacttagg ttgaacttat tatttcttat ataaataaaa aagagatgta
    19081 atattaaatg attatcatca tattattaca tctctttaaa tctaaatata taaaatatat
    19141 tgtaattacc aattaattat tagaaactta tctaaaatat ttaactccag attcaaatat
    19201 cttttggtct ttttcaccta taatgttctt aaatacttcc tcaccaattc tctcagaatg
    19261 tcccatctta ccaaacactc taccatcagc actagttata ccttcaacag catagaatga
    19321 accatttgga ttaaactcaa tattataagt agcttcatta ttaaaatcaa catattgagt
    19381 cgctacttgt ccattagcaa ttaattctct cataacatct tcactagcaa caaatttacc
    19441 ttctccatga gaaattgcta tattatgaat atctccaaca ttagtttgag ctaaccaagg
    19501 agatttatta gatgaaattc tagttctagc aatcttagct tgatgacgac caatattatt
    19561 gtaagtaagt gttggagcac tttcacttgg aactcttatt tctccatatg gaactaatcc
    19621 taatttaatt aatacttgga atccattaca gatacctaac attagtccat ccttttgagt
    19681 tagaaattca tttatggctt cttgaactct cggatttcta aatacagtag ctataaattt
    19741 accagaacca tctggctcat caccagcact aaatccacct ggtaacatta ttatttgaga
    19801 agattttatt tcatttacta ttgtatctat agaatcttct atatctttat aagttaaatt
    19861 cttaaatact cttatgtttg cattagctcc tgctctttca aatgctcttg cagaatcata
    19921 ttcacagttt gttccaggga atgctggtat gaatactctt ggagtagcta tacttagtga
    19981 tgatttttta gcttctcctt gtgatatata acttatagtc tctatctttg attttacttc
    20041 ttctgcttta gttgggaata taggctctaa agtactacaa tgtgatttat ataattcatc
    20101 taatgcaagt tcttcacctt tgataaatat actagcttct ttaactgtac ttccaagcac
    20161 aacataattg tatccttcta acatacttaa atcatcattt gctaattcaa gtactatatt
    20221 tccataacta gcctcaaata atttatcatc tttaaatgct ttttcagttt cttcactaaa
    20281 cttaaatcct attttattac caaaagccat tttacttatt gcttcaccta cacctgcaaa
    20341 tcctaatgca tatgttgata aaattttatt tccatgtata agctctctta ctttgtctaa
    20401 gtttctcttt aattcatcaa aatctataac atcattttct gccttgttta cgcataacat
    20461 tactactttt gaatctattt ttttaaattc tggagatact actttcttcg catctactgt
    20521 atcaacagca aatgaaacta atgttggtgg aacttcaata tctttaaatg taccagacat
    20581 actgtctttt ccacctatag caggtatctc aaatttactt tgtgcataaa atgctcctaa
    20641 aagtgctgag aatggtttac cccatttttc tggattgtta ccaagtttct caaaatactc
    20701 ttggaatgta agtcttgttg tactgtaatt tcctccaatg gcaaccaatt tacacacaga
    20761 ttctactact gcatacaaag caccatggaa tggactccat tttcctacct ttggattgta
    20821 tccataagtc attattgtag aagtattagt ttctcctcca agcactggta tcttagcaac
    20881 cataccttgc gttggagtag cttgatactt tcctccaaat ggcataagta ctgtattacc
    20941 accaattgta ttatcaaaca tctctactaa tcctttttga gagcaaacat taaggtcaga
    21001 caataccttt ataaatttgt ctttcatact tgcacactta acatctttta ctatttcatt
    21061 agagctaaag aatgtatttt cttcatctac tttagtaaca cggacatcag tagtttgttt
    21121 tactccattt gtgtccaaaa actctctatt tatatctaca attgcttttc cattccagaa
    21181 cattcttaaa tatcctgtat ctgttactgt agctacatgt gtagcttcta agttttcttc
    21241 tactgccaat tgtataaatt tatctttatt ttcttttttt atagctactg ccatacgctc
    21301 ttgtgactct gaaattgcaa gctcagttcc atcaagtcca tcgtattttt taggcactaa
    21361 gtctaagttt atatctaaac tatcagcaat ctctcctata gcaacacaaa ctccacctgc
    21421 tccaaaatca ttacatcttt ttatcatttg agcaacttct ttatttctaa agaatctttg
    21481 tatctttctt tcgttaggag catctccttt ttgaacttct gcactacaag ttaagattga
    21541 ttcttcacta tgctctttag atgaaccagt tgctcctcca cagccatctc ttcctgtttt
    21601 tcctccaaga agaactatta catctcctgc ttcaggtctt tctcttacta cattttcctt
    21661 tggtgctgct gctataactg cacctatttc cattctcttt gcaacaaagt tttcatcata
    21721 tacttctgca acttgtccag ttgtaagacc tatttggtta ccatatgaac tatatccatg
    21781 agcagcttct gtcgttattt ttctttgcat aagttttcca ggtaaagtgt cctctaaagt
    21841 tgttcttggg tcagcacttc ctgtaacacg cattgcttga taaacataac ttcttcctga
    21901 taacgggtct cttatagctc cacctaaaca agtagctgct ccaccaaatg gttctatttc
    21961 agttggatgg ttgtgagttt catttttaaa cattactaaa tacttttcca tcttaccatc
    22021 aacttcaaca tctacattta tactacaagc atttatttct tcactttcat ctaggtcatt
    22081 taatttccca tttttcttta attcttttac tgcaacagta gctatatcca ttaaacatat
    22141 atctttatgt ctgttcacat atacattgtc tcttgagtta agatacattt gatatgcttc
    22201 ttttactata tcattaaact taccatcttc tatctttaca ttttctattt cagtcataaa
    22261 agtagtatgt ctacaatgat cagaccaata agtatctaga acttttattt ctgttatagt
    22321 tgggtttctc ttttctgtat ccttaaagta tttttgaacg tgttgtaagt ctcctaaagt
    22381 cattgctaat cctttttcac ttacaaatgt tcttaatcca tcttcatcta aatctataaa
    22441 tccatctaat acttcaactg ttgttggtat ttcagtttcc atttttaatg tttctggttt
    22501 ttctaaagat gcttctctac tatctactgg atttatacag taatctttta ttttagaaaa
    22561 ctcttcgtca gttattttac ctgataatat ataaactttt gctgtattaa tagcaggtct
    22621 tatcccttga tttacaattt gtacacattg agctgcccaa tcacctcttt ggtcatattg
    22681 accaggaaga aattctattg caaatactct ttcatcattt aattttggta tttcttcata
    22741 atatactaca tctagatttg gttcagagaa tattgtcttt gctgcatttt cataaacttc
    22801 ttctgatatt ccttctacat catatctatt taatattctt aaattttcta tattatctat
    22861 gtgtaaactt tccaccaagt ctttttttaa agctttagcc tcaaggtcaa aaccttctct
    22921 tttttcaact agtactcttc taaccattga gtctttattt tcagtattca acatacctta
    22981 tcctccattt tagaaaaatt atcttcctat gtctgttcta tagtgcattc cttcaaagtt
    23041 tatcttcttt atattttcat aaactttttc tgctgcatct ttaactgtag ctgactttgc
    23101 agttatacca attactcttc ctccattggt aactaaatta ccatcaagca tcttagtgcc
    23161 gctatggaat actactatat catcatccaa gttttcaaga ccagttataa ttttcccttt
    23221 ttcataactg tctggatatc ctcctgatgt aagcataaca catacagctt catcatcact
    23281 ataatttatt tctatatctt ttaacttatt atctagtata gattccatta tctcatgtaa
    23341 atctgtttct aatctaaata aaacagattg tgtctcagga tccccaaatc ttacattata
    23401 ttcaagtact ttctctccat cttcagttat cataagacca acaaataaaa tacctttaaa
    23461 atcaagtcca tcttttttga aaccttctaa agttttttct aatactgtct cttttactct
    23521 atttgctaat tcttctgtat atatttcact aggtgaaaaa gttcccattc ccccagtgtt
    23581 tggcccttgt tcatagttgt ttactttctt atggtccttc gcacttgcca taggaactat
    23641 tgtatcatta tctacaaatg ctagaattga agtttctatt ccttttaaaa actcttcaat
    23701 tactatttta tctccagcag ctccaaactt cttatctgac atcatttctt ttaaagttgc
    23761 tatagcatct tctctatttt caggtattac aacacctttt ccagcagcca atccgtccgc
    23821 ttttattact actggatatc caaagttatc tatttctgat atagcttctt ctaagtttgt
    23881 atattcttta tattttgcag taggtatgtt atgttttatc ataaattctt ttgaaaaagc
    23941 tttgcttcct tcaagttgag cacatttttt attaggtcca aatactctta atccttcttt
    24001 ttcaaactca tctactattc ccataactag tggtacttct ggtcctacta ttgtcagacc
    24061 tatttcattt tctttggcaa attttaaaag ttcttctata ttagtatcac ctatatttac
    24121 gcattctgct acatttgcaa ttcctgcatt acctggtgca caataaattt tttctacatt
    24181 cttttctttg cttagtttcc agcatatagc atgctctcta ccgccaccgc ctacaaccaa
    24241 tatcttcata atatcctcct tagtgtttga agtgtcttat tcctgtaaat accataacca
    24301 tatcatactt atcacaagct tctattgaat ctttatcttt tatagaacca cctggttgaa
    24361 ctactgctga tattccatat tcatgagcta atgttacaca atcatcaaat gggaaaaacg
    24421 catctgacgc taaaacagct cctgtaaaat ctttatcttt gttattttca agagcatttt
    24481 ttaatgccca aattcttgag gtttgtccac aacctaaagc tagagtttgt ccatttttta
    24541 ctattgcaat tgcatttgat ttcatatttt ttactactct cataccaaat tccatatcct
    24601 taagctgtgc atctgttggt tttgcctttg ttacattttc acttttttct gctaatatat
    24661 tatttctatc ttgtatcaat aattttccat ctaaatattt catttcatat ggttgtaaac
    24721 tattttctat tttagctaat tttaatattc ttagattttt cttttgctta agtatctcta
    24781 aagcttctgg ttcaaaatca tatgccacta ctatttctaa gaatatttca tttaatttct
    24841 ctgctgtagc tttgtctata gtagaagtta ttcctactat tccaccaaat attgaaacct
    24901 tatccgcttc ataacatttt gtatatgctt caaaggcatc ttttcctaga cctaccccac
    24961 atgaattagc atgttttata gctactgaaa ctacttcttc gctatcttta aattctctca
    25021 ttacttctaa acatccatgt aagtcattta agttgttaaa agaaagttct ttaccgccta
    25081 attgctcata atttaagatt ggattttttg catttgattg tgaatataag aaaccatttt
    25141 gatgtggatt ttctccatat cttaaagttt gttctttttg gaaagttaaa tttaatatgt
    25201 cagggtacgt atctccaact tctccagcaa aataactaga tattaacgcg tcatatcttc
    25261 ctgtagttga gaaagcttta tatgataatt tttttctgtc ttctaatgtc attgtatctg
    25321 tttttaattt ttctattata gaatcataat cttttatatc tactactact attacatctt
    25381 tatagttttt ggcagcagaa cgtatcatag atggaccacc aatatctata ttttcgatta
    25441 tttcatcatg actttttcca gcttttaaag ttccttcaaa atcatataga tttacagcta
    25501 ctaaatctat tgaatgtatt ttatgttcat ttacagtttc tacatgagat tctttatctc
    25561 ttttataaag tataccacca tgtatatatg gatttaatgt ttttactctt ccatctaata
    25621 tttctggaaa atttgtaaca tcttcaactt gcattacatt aacaccattt tcttttaatg
    25681 ttttaaatgt atttcctgta gatataattt cataatctaa cttatttaac tctttagcaa
    25741 attccactac accagtcttg tcagttacac ttattaaagc tctcttactc atttataaac
    25801 actctcctac cttgaagttt taatttattt tcacaaaaaa gacttatact ttcttttaaa
    25861 atcctatgtt caacttctaa cactcttttt gcaagtgtct ttgcatcatc atcttgattt
    25921 accttaacta catcttgcat tatgattggt ccagtatcag caccttcatc aacaaaatga
    25981 actgtagcac cagttacttt agctccgtaa tcgataacac cttgatgaac tttttctcca
    26041 tagaatccag ctccacaaaa tgaaggaatt aatgatggat gtatgtttat cattttgttt
    26101 ctaaactcat taaccagctt tgggctgatt atttttaaat aaccagccaa aactactaaa
    26161 tctattttat tttccttaag aatttctatt atcttgtctt catcagtttc acaaattgct
    26221 tttatgttat gatttttagc tctctctagt ccataagcat cttgcttact tgaaataact
    26281 actttaacct gaccttttat ttctccagac tcagtgccat ctataactgc ttgtaaattt
    26341 gttcctccac cagatattaa aactcctata tttagcataa ctctacacct tcatgactat
    26401 ctactacttc accaattata tacgctttat cttctaataa ttcactgtat tttttatcta
    26461 cataagcagc attgtcattt tctctattat ttataaagtt tacaacatca tcagcatttg
    26521 ctttatctac tattaaaact agcccaatgc ccatgttaaa acttttatga agttctctct
    26581 cattaacagc attaaaacct tctatcattt taaatatagg aggcttctcc cacgattttt
    26641 tattgatatc tagtcctaag cctttaggta taactcttgt tatattttct ataagtcctc
    26701 caccagttat gtgagctatg gcttttattt catgtttagc taatacatct aaagccaatt
    26761 taacatatat tttagttggt gttaaaagtg cttctccaac tgtcattcct agttcttcaa
    26821 catattgttc cattttataa ttgtaagttt ctaaaaatat ctttcttata aaagaaaatc
    26881 cattactgtg tactccactt gaagatatac caaccaatac atctcctgac ttaacattat
    26941 ttccagaaac aatcttttct ttgtctgcta ttcctacaga aaatcctgct aaatcataat
    27001 catcttctcc atacattcca ggcatttcag cagtttctcc accaattaat gcacaaccag
    27061 acattttaca accgtctgca attccaccaa ctattttttc aatatgctca ggaactaatt
    27121 tcccaagagc tatatagtca aggaagaata atggttttgc tccttgacaa attaaatcat
    27181 tcacacacat tgcaactaaa tctattccaa cagtatcatg cttgtccatc atttgagcta
    27241 tttttaactt agttccaaca ccatctgtag atgcaagcaa aactggctct ttcataccta
    27301 caaaatcttt taaactgtat aatccgctga aattacctaa atctccaact acatttccat
    27361 cataagttcc ttttatttta ccttttataa ggtctactgc tctatttcct tcatctatat
    27421 ctacacctga ttctttataa gttaacatga aagtagttcc tccttatcaa ttttcttaac
    27481 tgggtattct ccatcaaaac aagctttaca gaaagtagac ttgtgttctg ttgcatctaa
    27541 cattccttct atatctagga attttaaact atcacatcca atgtattctc tcatttcttc
    27601 tacgttgtta cttgatgcaa ttaatttaga gcgatttgga gtatctattc catagtaaca
    27661 agaataagct actggaggag aagttattct aagatgaatt tcttttgctc ctgcttctct
    27721 taaagatttt actaattgtt tagatgtagt tcctcttaca attgagtcgt ctactaaaat
    27781 tatagacttt ccttttatta ttgttgaaag aggatttaat tttattttta ctgctatttc
    27841 tctttcttct tgtgtaggtt ttatgaatgt tcttcctaca tatctatttt taactaagcc
    27901 ttcacttatt tttaatccac tagcatttgc ataaccaata gctcctggcc aacctgaatc
    27961 tggaactgga actaccatat cagcttttac agtttcattc tgagctaatc tttcaccaca
    28021 tttaattcta aattcatatg catttacatt gtctatagtt gcatcatttc ttgcaaagta
    28081 tatgtgttca aatatacaac ttttcttaac tggtttataa ttttctgaat aaaaatatga
    28141 ttttagttct ccatctttaa ctactattat ctctcctggc tctacatctc ttataacttc
    28201 tccgccaagt atatctattg cacaattttc tgatgcaaat atatattcat cacccttttt
    28261 accaagtaca agaggtctga atccatgtgg gtctctaact gcaaccaatt cttcttgact
    28321 cattattaca agggcatatg caccttttat ataatccatt gtcactttta aactttctac
    28381 tatatcccct ttgtaatatc tcgcaagtat atataaaatc acttctgtat ctgaattagt
    28441 ttggaacata tatccatctt cttctagcat atcttttaaa taatttgcat taactagatt
    28501 tccattatga gctatggcta attgtctttt tctacaactt ccaactaaag gttgacagtt
    28561 agaaacatga cttcctccag ctgttgaata tctaacatgt ccaatcccta tatttccttt
    28621 taacttagat aattcacttt ccttaaaaac atctccaact aatcccatat ctttcttgta
    28681 cttaatttct ccatcatcta aaagagctaa accacagctt tcttgccctc tgtgttgcat
    28741 agaatataaa gaataatata gttctttagt tacatcttta ttagagtaaa tccctaaaac
    28801 tccacacata catttgcctc ctatttatta ttcattcttg aaagtacttc cgtataacct
    28861 tctacaaggt ctccaaggtc ttttctaaat ctgtccttat ctaacttttc atttgtgttt
    28921 acatcccaaa gtctacaagt gtctggtgat acttcatctg ccaatattat atttccttca
    28981 gaatccttac caaattctat tttaaagtca actaacttta ggtttaattt taagaaaaat
    29041 gcttttagta actcgtttat ttttaaagtt tcttctctta agaactttaa ttcttctcta
    29101 gttgctaact tcattgctac tgcataatca tcatttaaca ttgggtcacc ataagcatca
    29161 tttttgtagc ttatctcaaa aattggctcg tcgaaaacta caccttcttc aagaccaact
    29221 cttttacaaa tagaacccgc agcaatattt cttactatta cttctaatgg taaaatctct
    29281 acttttttaa ctaacatttc tctatctgat aaactcttta taaagtgagt atttatatta
    29341 ttttctttta acatttcaaa gattattgtt gatatcttat tatttagtat accttttgaa
    29401 gatatttctg cttttttttc tccattaaat gctgttgcat catctttata ataaacaaca
    29461 tattcatttt cattatctgt agaatatact tgttttgctt ttccttcata taataacatc
    29521 tctgctaccc ccttaaattc ttgtcgtctt ctaacacctt ttgtgccatc tcttctctat
    29581 atgatttcaa atcctctttt aattttgggt attttaaagt cattatttgt aatgccaata
    29641 aagctgcgtt taaacctaag tctattgtaa ctgttgcaac tggtattcct tttggcattt
    29701 gaactattga taaaagtgag tctagtccat ccatagttga tgatttaata ggtaatccta
    29761 ttacaggaat taatgtttgt gaagctatta cacctggtaa gtgtgctgct tttcctgctg
    29821 ctgctattat tacatcagta tcatcttcta tttcttttag gaatgttgaa agttgttctg
    29881 gtgttctatg agctgatagt gctcttgcta caacttctat tccatatttt tcaagcagtt
    29941 ttatcccttc ttctaacttt ggataatctg attttgaacc cataactact gctactttca
    30001 ttagacttcc cctctccgtt tataagttat tttacactat aaatcattcg tatgattata
    30061 cgaattatac accttaaatt ttatattaat atacatatat tatcaactct tttttgaaat
    30121 ttttaaaaaa aaatatttct tgtatttttt tattcgtcac gtatcgaact ttatttatgt
    30181 ttttttatta aaataaaaaa cagattatct caaaattgat ttttctaatc agattttaaa
    30241 cttaatctcc tttttaaaat tttgtatatt attttttatt acttattttt taattaatat
    30301 cttaaattta tagtcttgtt ttttaactct gaatgttact ttcgcttgga taaaactaat
    30361 acatagttta ttctttatct aagtaatagc taaaacttaa agtgatgata ttatatagat
    30421 agatataatt ttatttaaag gagaatctgt atgttagaag ttataaaaaa tagacgttca
    30481 ataagagcat atataaataa aagcgtagaa gaagagaaaa taactgaaat actaaaatca
    30541 gctatgcaag caccttcatc aaaaaatgct caaccatggg aatttatcat agtagatgat
    30601 aaggaactac taaaaaaact ctcaaaatca caacatagag caaagcatat agaatttgca
    30661 ccattatgta tagtagtgct tggaaataga gataaattct taaaacctgg taaatggata
    30721 caagatttag gtgcttgcac acaaaattta ttattagagg ttactaacca aggtctagct
    30781 gcttgttggg taggagtatt tcctaaaaat aaagtggtaa ataaagttag acaaacttta
    30841 gacttaccag taaaattagt accttattca cttatatcta ttggatatag tgaagaaaaa
    30901 aatgaattta tagaccgatt tgatgaaaat aaaatacata gaaatatata taaaaataga
    30961 tagtgtttta tttcctctaa actagctggg acataaaaat ctcctatata tactagtgct
    31021 atattagggg atttttcttt gttttgtaat tgatattaaa aacaagactt aagaccttgg
    31081 ttttattttt aaaattcatt ttatctaact ataaaattta agatatagaa agtacagcag
    31141 ttaattattt taaatttgtt gagaatatct taaaattagc cattttcctt aaaatatttt
    31201 agtaaattaa tacttcctat caaaacttca gtaaaaatat accacttaat tattattaag
    31261 attaatcttt gtacaaaaat tcatattgcc tcttaaaaga gtacatacaa ataaatttta
    31321 tctaattggc atttaaataa actatcttac aagtatttaa tagtaactct cctacaaaat
    31381 tttattagta tatgagttaa ctaaatttta atataaagag aacataaaat actgtaaaaa
    31441 agtgttatgg ttatttcgtt gaaaagaaag acatactatt aaagcataaa ttaaaatata
    31501 ggagatgatt aaatgagaat actttcagaa actgaaaaaa tatccttagc agcagttata
    31561 aaaatggaat ctgatggatt acttatgcaa agagcaataa atatgttaat atcagatgaa
    31621 gacctaaaaa gacaatcaga atctagtata ttagcaacag aaggaagaat taaagctata
    31681 caacaattta tagttgaaaa tgaaatacta atttctgagg aggtatagat aatatgagaa
    31741 atatgataga aaatctaatt aagaacaata cttatataga cgataaagta ataatgttaa
    31801 gtatgctttc atctgctaaa gcatctgcca atatgtatct taattctgca ttaacaagta
    31861 gtacacctga attaagagct atatattcag cttcactaac tcaaatggtc gaaggtcata
    31921 cagcacttac agaattaagt attaataaag gatggtttaa accatatgat aaacctattc
    31981 aacagttaac atgcagttat aaagaatccc aaaatgttat tgactaaact aaataaaaca
    32041 tatttctaac tatttcactt taattaaata ataaaaaggg gatattcaaa ttttttaaga
    32101 tttccccttc ttctaaataa tctatttttt aatataaaat agattgctta caatctaaag
    32161 atataatttt atatagttat tattaattat tttagacaat tctatttatt aacatttttt
    32221 actacatatt tttataaaca aagacatcaa atccttatgt tccaaaatta aaaaatcagg
    32281 cttataaaac accccttcta cttcatgtct cctatggttc aaccatatac tcttccaacc
    32341 agctttttta gctcctaata catcatttct ataagaatca cccatataat atgtattttc
    32401 tctatctaaa ttcataacat tctcagctac tctaaaaata ttagtatctg gcttagaaaa
    32461 tccaacctca ctagatataa aaatattatt tttatcaacc cagttttcaa tatttaactg
    32521 ctttaacttc attctttgat gagttgatgg accattagtt ataattccta gattaatatt
    32581 cctttcttta gaaaaattta aaacccactc aacttctggt attagtgtaa tttgtgattg
    32641 ttggtatata tactcatctt gaaaactttg tgcatctttt tctgtaatgg aagttcccaa
    32701 ttcctcaaaa gctttcatta ttctataaat atgcatttct ttgattgcca tttctccatt
    32761 ctcagtcata tgaaatacct catcactata ttttctacta ctcatatata agtcttcaat
    32821 agaaatattc tttattgaag aaaacacttt attatatgca atataaaaag gtgttaattg
    32881 attatataaa gtatcatcta catcaaaaat taaattaatc aaaatattag tcctcctaaa
    32941 tttaatagtg gtaagtataa ttataactat aaatacctag tataacaata ctcaccacta
    33001 taagtcccat tatttattta aaatccttgt agtaacacct tttaagttat aacattccgc
    33061 ataatcacgt aaaggacgtt taatgtggtc tataacaaat ctttgaccat caatattatt
    33121 cttacttaaa ttagcataaa gcacttctat ttcttttttg acatcatcat tattaaatgt
    33181 ataatgccct gcaatatcta atatttcatt agataaaact tcattcttaa atatatcttc
    33241 agtagttaga tttttttgtt ctcctattac ccattttctc caacgtccac attcaattgc
    33301 attaacaaga agaacatgtc tgatattaga agttttatca actaaaccct ctttttctaa
    33361 tttacactca acttctgcta attttaaata tgctctagtt tcttcagtac catattgagg
    33421 agctacattt gtagcaataa tactagatgg tatatgttct aataaagtta catcatctaa
    33481 gtaatcacca ttatgctctt ttaaaccaac tccatatttc ttagccattt gagctaaatc
    33541 ataagcattt ctaaaattaa aagttcctac ttgttctgtc tttctagtta aagtaccagt
    33601 ttgaccaact ataaatgttg gcattggcaa acctctctta tcaagttcaa cttttagtct
    33661 agttataaat gtttcatacg tctcagttga agtcagacct ccattggttt cctctgtacc
    33721 aacttcataa cctatttctg gtagattgag tctttttctc tcattttcac aatattcaat
    33781 taattctaca gttctttcta gtactacatc tagtggaatt acttttccaa tttcaaatgg
    33841 gtcttttgta gggtcaatca ttaataggtc aaaacctgct tcaatgtccg ctaaataaga
    33901 tttttttcct aactccattg cctcatcaac aggtaaatgg tcatttctct ccttatctct
    33961 ttgccatggt ccaccatggt ctctacataa ataatataaa ccatcaaatc caactttatc
    34021 ggcaatcttc ttaatatctt ttgtaaaagt ttcttgattc caaccattta catagccgcc
    34081 acctagttca tctgcatcta cttgatttct gctcgcaata aacattaaag gaaaatcatc
    34141 atcttttgct aattcaaaac ttgcttgtaa caagtttggt gacataggac caattcctaa
    34201 aagagttgca cttttcccct cttcttgtaa ttctagtaat ctttgtacga cagtctttat
    34261 tggtaacttt ttcatgacta tatctccttt tattctattt ttaagatatt ttcaaagatg
    34321 tttttaataa aaatctcctt aaaaacatct ttgagttata tgtaaaacaa atcaattttt
    34381 attgaaaagt atcttttatt ttcttaatta atgcatcaga tttcttaatt atgtcagata
    34441 cactcattct tactactttc tttcccttaa aacgttccat atttctaatc tcaacatcat
    34501 tagtcaatat tacacaagtt gcttcctcaa tctctttaag cgacaattca ttatctatcc
    34561 cagagcttcc ttgagtttca acctttattt ctatgccaaa ctttttggct gataattcaa
    34621 gtgattcagc tgacatgtaa gtatgtgcta ctccactagg acaagatgtt actgctacaa
    34681 ttttcatatt cttatctcct ttagacttat atttttatat atttactttt ttatacaaat
    34741 tcctttctta tttatattta taattcttca aatattaatt caatcgagtc atctaaatcc
    34801 tcttcaattt cttcatcttc tttcacgtct ttttttacta atttcattaa tattgcaaca
    34861 actactgctc cagttaatac tgatgcaata taccccaacc tattgtcaac tacgggtaat
    34921 actatcagac ctccccaggg tgcatgattt aaacatccta ataaaaaagc cataatgttt
    34981 cctataatcg acccaaccat aatacaaggt attactctaa cagggtctga tgttgcaaat
    35041 ggaatagctc cttctgtaat tccaacacat cccattaata gtgaagcttt tcctgaatct
    35101 ctttcttcct tgctaaattt ttttggaaac aataatgtag ctaatcctaa accaattgga
    35161 ggtatacaaa ttgcaacacc aactcccccc attaaatatg gaagtgtgtc aacttgagtt
    35221 tgagcaaaag tagctgcaac cttatttata ggaccaccca tatctgaagc tatcattcct
    35281 cctaaaattc ctcctaaagg aattttagaa acaccagaca ttccatttaa ccaattagtc
    35341 atccatgtca taaaagaagc tataggttct ccaattaaaa atacaattat accacctgta
    35401 atcaatgtac caatcaaagg ataaataaat atcgaaccta aagtcctcat tgtaggagct
    35461 aatttaattt tctttaattg taaaacaaca aatcctgcta taaatcctgc tatgatacca
    35521 cctataaaac ctgcatttac ttcttttgct aaatatgcac ctatcatacc tggagctaat
    35581 cctggtttat ctgccatact aaatgctata tatccaccta atataggaat gaataaggcc
    35641 aagccggcag ctccaatttg attcagttta gccaaccatc ctgtttcagg aacagcagct
    35701 tgtccactta gagttactga taaagcaaat aatattcctc ctgcaactac aaatggaatc
    35761 atataagatg ttgctgtcat aagatgtttt tgcaaaaact ttaaaattct cattttaatc
    35821 ttccccctct attttttctt ttataagatt ataaacagtt tgttcatctg aacatctaat
    35881 ttgatttcta aaactttcat gcatcaataa acgtgctaat ttcgataaaa tctttaaatg
    35941 aagattattt tcattgtctt ttgttactga tattaaaaat attaaatcca ctttttctcc
    36001 ttcttctgtc cattcaatag gagtatcaag ttttcctata gtaatacaag agtttttaac
    36061 acccacactt tgactatgag gaagacctat accatgtcca atataagttg acaatgtttc
    36121 ttctctcttt aaaacatcct ctaaaaattg atttttatct cttaaatatc cttcttttga
    36181 tactacatca actaatgata taatggcttc ttcttttgaa ttagcatgta ttttaaatga
    36241 acattttaca ttaactaatt tactcataca gttacctcct tctcatattt atgaattaaa
    36301 ccttctgctt cttcctttgt ctttgcactt tctaacatag aaataaaatt agtcttataa
    36361 accattttag ttagattaat tattgcagga atttgctctt ttttatcttt gcttgctaaa
    36421 caaaatatga ttctaacctt tttatctcca aaactaactg cttctttaca tattaataga
    36481 cttatcgaac ttactttgac taatgaacta tcattaccat gtagtaaagc aaattcgtta
    36541 tcttttacag cataaaaacc cacatttttt aaaatatcta taatattttc agcatattgt
    36601 gatgatacaa aatcacaatt agcaagtata tttgaactaa tttttacagc atcttcccat
    36661 ttcatatctt catcaattat ccttatggta tcaattccta ttaaatcgcc taaattaagt
    36721 tgtctagata atgctctctc atcactgtat ccaagttctt gacatataac acttaatact
    36781 cttttttgag catcttcttc taaaaagtcc aacctttttt ttattgacat ataattagtt
    36841 aagacgttct ttctttttat acctttttcc tcaaacttca aataatcctc ttcttgtaaa
    36901 aatggattta tctcaataat tgttttaggc aagtctatta ctatctttga tgttgttata
    36961 atgaagtcaa tactttccca gtctttataa tcttgtaact tatatgatgg aatactgtcc
    37021 actatattta tctggtaatc atttattaga ctatctttta acatagcaat tacaccatag
    37081 ccaaatccac aaactaacaa tatatttttg tagttattcc tctcatttct atgtatactt
    37141 cctaaaaaat gtattgccaa aagtattgtt tcattttcat tctttatttg atttaaaact
    37201 ctgttctttt gaatagaaac cttagtagct tcaagtacat attggtactt atctggtatt
    37261 acatgaagta attcttcata aatctgtatg ttctgtttga ttctctctat caatggggca
    37321 atatgattta gtaatccttt ataaagaatc atatcttctt taaaactaat ctctaatatt
    37381 tctgacatat tgtaaattaa atcttgaact ataatctctg tactaatcag atccatatcc
    37441 tcatttaagc ttgcatattt atttgtaaaa aatacaaaac tctttaacag aaaaatttgt
    37501 tccttagtca actttttttg aataattgtt tcaaactctt ggtcaaactt attttcaaaa
    37561 catggctcac ttaatgaaac agattctaaa ggatgttttt catctgaatg tatataccat
    37621 gtaaaaagga aaatgtttga cacataccat gtgaaagatt catcatttaa agtccatccc
    37681 atttgcaata ataaattcat agaccaatta tatatatcaa tcaaagaaat ccccttgaat
    37741 attttttcaa tcacattcac taaatacttc tcaaatacac ttaaatcaga tttttcttta
    37801 ccaaaaagat aagaatatga ttttaatata ttcaatcttt gcttatatat aatgtcttct
    37861 tctccactta ttttaaaagc tttatcataa gtaatagtta tttgatttct ttctaattca
    37921 cttgataaaa cattaaggtc atttttaatt gaacttctag aaacctgaaa ttctttactc
    37981 aatttttcta agtttagatt ctctatttta aacattgata taattttcat aatagatact
    38041 cgctcagtta aggaaaatac atattcgtta ttatttatac atactacttg gaagttatca
    38101 ggaataatta acacaccttt gttcatcttt tgtattactg gcttgtttaa tgaacgcaaa
    38161 acaatattta aattatttat atcatatcta acttttcttt cttctatgct aagttgtttg
    38221 ctaatatcct tgtaacttgt cttatttttt ccttttagaa aattcatgat gagtaacatt
    38281 ctcccactta tcatattttc cctccttcca aaataattag agttattaat atagcaaatt
    38341 ttacattgtt atgtatgttt tagttttttt atctaaaaca gtaactatct ttctatattc
    38401 agaataatac aataaagctt tagccacaac ttacaacatt tgaaaattat ttttacaaaa
    38461 tctgcaaata cattttttat taaatatata aataaaaaag gcaagttatg ttttaattat
    38521 ataacttgcc taaatttttt atcttatcca tttaataatc agatattttt aattttatta
    38581 ttttcatatg caaaatcttc aaagtcagaa attagtagtg gtttagaaaa aacataacct
    38641 tgaatatagt cacatcccac attttttaag aactcaactt gctctaattc ttcaattcct
    38701 tcagcagtta ctttcatacc tagccttttg ctaagttcaa taatagactt aataactacc
    38761 ttaccacggt ctatattatt tttgctgaca aagaacgaac ggtctaactt aattatatca
    38821 actttaaaat cctttaatat tcctaatgat gaatatccaa atccaaaatc gtctatagaa
    38881 catttaaatc cattagaatg aatatcatct attatatcta cagcttctgg attttctaag
    38941 aaaatagatt cggtaagttc taaatctatc agatatgttg gtacattgta tttattgcat
    39001 ataagcttat atttttctaa aaacaattta tcattcaaat gttgacgtga tacattaaca
    39061 gaaattctca atacttcttt accttctttt atccatttag aaatcaattt gcatgtttct
    39121 tcaagtatat acaaatctaa cttacatata aatccacttt tttcaaaaag aggaataaaa
    39181 tcacttggat aaattatgcc tttttccgaa tttttccaac gcacaagaga ctctgcacca
    39241 ctaatttttc cactttttat atcaatttta ggttgtaaac acataaaaaa ttctttattt
    39301 tcaagggctt tttccattaa atttgaaatt tccttttcag atattaatcg ttttctttct
    39361 atatcactat aaaaagaaaa atcactttga tgagctttat taaagctttt tcttgctgta
    39421 tttgcatggt cttgtatcgt aattaaatct tcattatgat tttcaattgt gtaaatacct
    39481 gccgatattc ttaaaaagta tggattttct ctatttttat taaaataatt tatatcagct
    39541 tctattttat ataataactc caaggcttct agcactggtc tattcttaat aagcaaatag
    39601 aaaaggtctg catcatgacg gcacacaaat tcttcatttt catcaatatt tttatataaa
    39661 acattgtaaa gatgttttag tgtcaaattt ccctttttac tgccaaaaca atcatttatt
    39721 agcttaaaat cttttatatt tatagctacc atcatgtaag tactatcagg tgattctaaa
    39781 attaaaggtt ttactaagat actgaattta gttgaattta taccatttgt tatactatct
    39841 aaatatgcaa tattctctat ttgatttaca tattttcttc ttataatata taaaatcaat
    39901 gttataattc caataataca tgtaattata aatgtcatta ttattgtaaa agagtttaac
    39961 ttattaagtt caccaagtac agcatcttca ggtactataa gaacaaaatg ccaatcattt
    40021 aaaccttttg taattggata ataggttaat aggcattgta caccttttga tgaagttatt
    40081 gtaatatttc ctgatttatc atttttaaaa tcattaagca tcttttgtgc ccaaggttca
    40141 tctccagaac tcttatattt atggccaagg tcttttatta attcagtatt tttccctatg
    40201 aaaagaggtc ttccctgact gttaataatt tcaatagttc cttctccatt aaagttatct
    40261 atattaagaa tctcgtccat actttcttta ttagatacac ctatcaaaat actagacact
    40321 tcatgattat cattgaatat aggtacacta tagaaaacaa aacccattga ctctattact
    40381 tctgagtatt ctttaccttc aattcctttt ttgtaggaat cagtattctt taaatcaaac
    40441 ttttttccgc ttaaaaattc agctttaccc tctaaatcta ttattcctat atcataatac
    40501 ttgtaactag tctttctttt agataaatac tcatatcgac tatcaccttc atattgctta
    40561 ataaaatctg ccattattct taaatcggat aaaataaatt tgattttaga atcaacattc
    40621 attacaatct gtgtagatac atcatttaaa tagtttttag attgtttact agatatattg
    40681 tgtagtttta cagtattaaa aacagtaatt acaaggattg aaataataag tgaacataaa
    40741 attatagaca gaactaaact atttttctta taccttgata atttcatttt tttcctccaa
    40801 attataaacc tattttttaa tgtatattga cacttacaaa tttttttact aagaaccctt
    40861 cacactatat tatattatag tattccattt attaatattc aataatacaa tcttaacatt
    40921 tcattaatat ttcatatata ataattttat taaataatta tattatatgt aatttttaat
    40981 atttattgat taaaaacatt atgacatgtc atatatcaat aaagaactat acctaaattt
    41041 ggtatagttc tttattttaa taacaccttc gatattctat aaaaaatcta ttacaatagc
    41101 tactgcttat ctcctctaat aatatggcaa ttatatcata atctatagta ttagatagta
    41161 ttgattttga aatgatgtgt ccacctatcc aaacatcatt tctaattttt taacttgagt
    41221 agttgtagtt ttacaaaact taaatctatc atccattttt tatacaaata gctcttattc
    41281 ttactacagc aaatataaaa taaactatat ttgtattaca atttatcatt gtatcatttt
    41341 ttatttattt ttaaatttta tccgaaaaat ttagattgcc cttgcatata acaccatgtc
    41401 aggatatatc atccacttag agaggtgaaa tataaatgat aataaataac tatcaaaatc
    41461 aaagtatcat cacaaatcct aaaaaatttg aaaatgaata tcaatattta ccagatactc
    41521 caattgaatt attaaaagtg gttcaaaacc taataattca tggagaccaa ggaaaactat
    41581 acggtatatc ctttaataag caacaatctg atgaagaatt gcttcgtaca gttcctcaaa
    41641 tgttaaaaag aatatttgaa ataaattcta actcactaac tattccaaga accccaaaac
    41701 aaagacttgt tggaatgtgc agagattatt ctttacttct tgtgtccttc ctaagatatc
    41761 gaggttttga agctagaatg agagcaggtt ttgctaatta ttttgaaagt gaacttactt
    41821 atgaagacca ctggcttgta gaatactatg atactgcaac aaagagatgg ataaaaatag
    41881 atgcacaaat agatgacatc caaaaaaatt attttcaaat taattttgac gctcatgatg
    41941 tcagaagaat agatggcttt ttaactggtt cagaagcttg gataaaatgt cgacaagagc
    42001 atactcatcc agatgacttt ggatataata aaaattggaa aggttggcat tctgtaaagg
    42061 gtaatcttct acatgacttt aataatatga ttggtttgga attacttcca tgggatttat
    42121 ggacagaatt aagttccaaa aaatacaatc aattaacaag agcagaaaaa aatttattag
    42181 atgaaatggc agagatatta tcaagcggaa ataaaaaaat agaagacctt aatttgttaa
    42241 tagaaaaatt gcctgaagat tatttgaaat caatcttttc tcagttaaaa atattaggta
    42301 tttcggaaat aaaagaatta gctaatcctc ttgaactaga aaagaaattc aaatttacaa
    42361 aagcaataaa taaaccaatt aaaaattcac tttgtcataa taaaagctct atctatttaa
    42421 aaggaggaag acaaaacaat ttaaaagatg ttgaagttac aattccaaag aataaaataa
    42481 ctgtgattac aggtgtaagt ggtagtggaa aatcttcttt agctttcgac acgatttacg
    42541 aagaaggaaa aagacgttat tttgaaaatc taggtaatgg agcaaaactt tcagaacagc
    42601 ttcaaaaacc ggaatttgat ttacttcagg gattaacccc tactatagct atcgaacaga
    42661 aaaaaggaaa ccaaaatcct cgttctactg ttggtactct tacaagcatt tgggactact
    42721 taagaatgtt atttgtatca atcggaaaaa gttactgtcc atattgcaaa actccattag
    42781 aaaaaaagaa taatacaaaa aattattgtc cacattgcca aactattttt tctaagataa
    42841 atacttcaac cttcaatgct aactcccata ctggagcttg tcacgattgt aatggattgg
    42901 gttttaccta tcaagttaat cctcaattaa ttgtaaaaga cccaactatt tcaatattag
    42961 atggagctac ttactatttt ggaaaattga gggggaaaaa gccaaatggt aattggatgg
    43021 taggtgaatt atatgcaata gcaaaagata aaaatataga cctagatata ccttggaatg
    43081 aattgccaag agattttata gatgcaatct tatatggtac agacgataag atatacgaat
    43141 ttagttttga atcaagggga cgagaatcaa agattagaag acctgcttca ggagcaatca
    43201 accacattca aagacttttt agagaatcta gttctgaaaa taataccctg catcaatata
    43261 tgaacaaaat tccttgcaat acatgtggtg gcgaattgct gtgcattgaa gctaggttca
    43321 ctacaatcaa aggttatcgc ttccctgaac taacaaaaat gacgatagaa cagttatgga
    43381 attggttgtg tgaattgaca aatcaacttc aacaaaagga gctatcttta gtaaatgata
    43441 tattgacaga attaaaaatt cgagttagtt atctattaaa ggtaggtctt agttatttat
    43501 caacagatag aacagctcca accttatctg gaggagaact acaaagagta cgattatcaa
    43561 gtcagctagg aagtgaatta gtaggattaa cctatatttt agacgaacca tctattggtt
    43621 tacacccaag agaccataat ctaatcatca aaatgataga agaattaaga gataagggta
    43681 atactgttat agtagtggaa catgataagg atacaatact gtctgctgat tatattattg
    43741 atgtaggccc aaatgctggt ataaaagggg gcttcatcat tgcagaaggt acaacccaag
    43801 aaattataaa aaatccaaat tctataacag gcaaatattt gagtacatac aataagataa
    43861 gttcacaaaa taaaattata ccaagtaaat ggttaagctt aaagggatgc catgcaaata
    43921 acttaaaaaa tattgatgta gaaattccct taaactgtat gtgctctatt acaggtgtta
    43981 gtggttctgg taagagttct cttgtatttc acagtttact tcctgctcta gaagaaaaat
    44041 taaaacaaaa atctatccca aataaaaatt atactgaact tactggattt gatgctattg
    44101 atgattttat cttaatggac caaacagcta ttggaaaatc tagccgttct acacctgcta
    44161 catatatcaa tatttttgat gaaattcgtt ctctatttgc tgaaactcca caagcaaaac
    44221 aaaaattatt ggatgaaagc tattttagct ttaatagcaa aaaggggcaa tgcccaaact
    44281 gtcaaggatt aggtaaaaca aaaattattc tacaatatat agcagaccaa tgggtaactt
    44341 gtaataaatg tcaaggtaag agatataaaa aagaaatact gtctatacaa tacaagggaa
    44401 aagcaattgc agatatactt gacatggaag tagcagaagc aaaaacattt tttagtgatt
    44461 gctctgatat atatagaaaa ctctacttat tagatgaagt gggacttggc tatctaaagc
    44521 ttggacaaaa tacattagga ttaagtggag gagaatctca aaggataaaa ttagcaaaag
    44581 aattgggaac aaaaactaaa aaacgaatgt tatacatatt agatgaacct actactggac
    44641 ttcattttaa agatatagaa aatttgttaa ttacttttag aaaacttgta aatgagcaac
    44701 attcactttt aattattgaa cacaacacag aggttatacg tgcttctgat tggattatcg
    44761 atataggtcc agacagtgga atgaatggag gggaaattgt cgcttctgga actcctgatg
    44821 aaataaaagt aaatccaaat agtattactg gacattttat ataaaaacaa ctaaataaac
    44881 atggacttag acataaaatt atagaatatc ttatattatg gaagaattat tataatagta
    44941 gtataataaa aaaattaact ttagtatatt gtttgtgata aaatatgaat aatctataaa
    45001 atagagtata agaattatta tgttttataa tagagaaagg aaaatcaaat gagttggaaa
    45061 catataaaaa taccaaatgg agatacaatg attttttgta actcatttat tactagcaaa
    45121 ttatctatga atattattgt tacacacact cctattgtca ctacgcttga gatgcaaaaa
    45181 gcatacgaac catttactaa atataatgta aatatatttg cttttgattt ttcaggaaca
    45241 ggaaaaagtg atggatatga aaaagatttt tctcgcaatt caattataaa agacttagat
    45301 tctatcatta catacataga aaaaaattat tcttccaaca tacatctata tgggaataca
    45361 gggattggtg gcatgtttgc gcaatattat gcatgttcag acaataagtt aaaaagtttt
    45421 gctcagtttg cttgtgttga ttataaaaac acaacaggag taggctatcc ctatctcact
    45481 gttaaattat taaacctttt ctttaagttt cttcctaatt ttcacataac agtaaaacca
    45541 cctaaatata aaggttataa tgaagtctat gataatgaat tttataaaat atttgaagag
    45601 aaaaatcctc atatttggaa aaatagtaca aagataatta atacaatgtt agagtgcttt
    45661 attgcagaag atagtaagat aaaagatgga gtcaacatac ctactcttgt ctttaaaaca
    45721 cttcatgata gatattttaa aacccaatat tttgatagct attataattc attaaaatgt
    45781 aaaaagaagc ttgttgaaat taataatgtt cataatagtt actatataga tagtgagaaa
    45841 ttttgccaag aagcttatga ttggtttgta gaaaaccagt gatataatct aaatgttata
    45901 tggatttctg attatctttt cagaatttat taaaaatcca aatagcctta ctttattggc
    45961 tatttggatt taagatataa aaaaatagat atcctttact tatcaaaaac ttataaataa
    46021 aaaaatattc ccagtatacc acttattatt ataatgacaa taggattaat tttccactta
    46081 cgtactatca gtagcgtaac aaaaaagatt attagtgcag tccagtttag catctcaaat
    46141 atagaattga agtacacttt attcgtacca aataatgcaa ggattataat tgtagccgca
    46201 gctgagataa ttaaacctaa tgaagctgat tttagaccat ttaatatttc aaatatataa
    46261 gtagagttct gatgcttttg aaaaaaatta tataaaaata gagatattac tacaccacat
    46321 aatacacaac ctaaagtggc tgcaactgct ccccaaaacc ctccaatttg aagcccaaca
    46381 aaagtagaag tattaacagc caatggtcct ggtgtcattt gcgaaatagt tataatatct
    46441 gtaaacattt tctcacttat ccatccagtt tgatttacta ctttctcttg gattaaaggt
    46501 atgattgcat agccaccacc aatactaaac aagcctattt ttaaaaatat aaaaaataag
    46561 ttccaaattg tattcattta tttttcctct tcttgtagaa aacttgcatt acacatacac
    46621 aacagcaaac agataatata aaaattacat ttatactcat cacaaagttt gcaaagaaag
    46681 caataggtat catagctgat aaaaatagag aacgttgttt taatatcata gagcacatgt
    46741 caataatcaa atccacaatt agtgcagcaa caccagcttg cattcctttt aatactgcag
    46801 ttacaatagc attagatata aaagtggtgt aaaatgctga tactaatcca agaataacga
    46861 gtggaggtat tatagctgct atacaactaa tgacaacccc taaagtacca gctactcgat
    46921 aacctgccaa agcagataaa ttaattgcaa tagctccagg agttgattgt gcaactgctg
    46981 ccatactcat caaatcatct tctgtaaaga aatgcttttg atttacaaaa taacgtctaa
    47041 tcataggtac aactacatac cctccaccaa aagtgaatgt gctaataaac aagttaataa
    47101 ttaaaagcca aaaatatgtt tttctgcttt gattcaaaat attcctcctt cttttcactt
    47161 catattattc agtatacctg ttgctttgca atctgtaaaa tgatagaatt taatggaatt
    47221 aatcaatttt attcatgtct agtgaggata tttattatga taaacatacg atatttagag
    47281 attttagaag caattgaaca aacaggtact tttacaggtg ctgcaaaaaa attacatatc
    47341 acacaatctg ctgtatctca tgctatagct gaacttgaac aacaagctga aacatcatta
    47401 tttgacagat taccacgagg ggtatgtctt actcgctgtg gcatttctct tttagaagaa
    47461 gcacgcagta tcttaactgc ttgtcgcaat ttagacaaaa gaatcagtcg tttagaggaa
    47521 tgtacaccta taaacattgt ttctagcatt acaattgcct cttttttact tccaccaatt
    47581 ttaagtcgac taaaaaattt gttcccatct cttaaagtga atgtacgtgt cgaaagtgct
    47641 aattcagcca tggatatttt aaaaagtgga gaagctgata tatgtttttg ggaaggtata
    47701 gaaccacagg gggcattccg tacaatttta ttaggttcct ataagttatg tgttgcatgt
    47761 gctccaactt ttcctttatc tgaacaaacc ttatctctac gacagttatg cacgtttccg
    47821 ttattgttac gtgagcaagg aagtgctatt cgcgatactt tagacagtgt attatctctt
    47881 tcaaaccaaa aggcttatcc tatttgggaa agtgttaatt cttttgcact tattaaagct
    47941 gcagaagcag ggttaggtat aaccattttg ccagagaatt tattattaga ttcactctta
    48001 cataaaaaat tgcgattgat tgaattagat gggatagata tggaaaataa aatgttggca
    48061 atattacata aggataaaaa tattacacaa cctttgaaaa taatattaga taatataagc
    48121 aatgtacttt aatttaaaga tttaattttt taacattcta ttttttagca taaaaagtgt
    48181 gtactattcc ttaatgttgt gattttaatt ataatcactt tttaaaataa tacacacttt
    48241 attttaagta caatctaaac ttaaataaga taaaagtttc tattgtatat gttttaaatt
    48301 aatactatat tcttgtttac ctttttcaat tttaaattta tcatttttat taggataaat
    48361 agttacaaca ttatctttat actctgattc atcacatttt atattaattg gcataacttc
    48421 agcttcacca tatacatagc taacaggctt attattattc attacaacaa tctgcaccat
    48481 gtcatcaccc tttggagatg atatatttct ccatttatat cctattgttt tatatatatc
    48541 atctgcagat gttccagatt taaaaacata tattttatcc cattcaggag tctttacttc
    48601 tgataatcgt atttctttct tatctttact tactttatta attgaagaaa taaattctcc
    48661 aaatttgtca ctttcttgat ttttttcaaa tttaatcaat ttactagatt tgtcaactat
    48721 attccattta tcattggttt tttctaattt aaaattaatc ttagaaatac attctccatg
    48781 ttttcctggc tcagtgacta taacattttc tcctgattta tttttataat catgttgctc
    48841 aatttgaaca tgattatgac ctgcaactat agcatcaatt ccatcaactt gttgagctaa
    48901 atcttgtatt ctatttcctg ggtttctagg cttcttaggt ttctcacctg tatgtgccac
    48961 agcaactata atgtcaggct tctctttttc cattatacta acccactttt tagcatcttc
    49021 tactaaatca ttcatataca attcaccatt gtagccctct tggtctttta gttctaaaga
    49081 cttagagtca acaagttttc catctttcca ctgtttcctc tcaccaactt cttttatagt
    49141 aagccctaaa attcctagtt ttacgtttcc ttcaggtgta ttaatatttt ttattatgta
    49201 tggttttgta taactgtctc catttttttt gtaagtattt gctgataaaa tatttatttt
    49261 ttgcttctca aaatcagata tcatattatc cagataaaat ttattgttgg atataaattc
    49321 atgattccca agaactgcaa catcataacc tacctctttc atatcttttg aaataggcgt
    49381 ctcaatgtaa cgctcagtgt tactatcaat atccttattc ctagcactaa aataactatc
    49441 catgctagaa ccagatactt ccccac
//
LOCUS       DAFNNB010000009        49207 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0009, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000009 DAFNNB010000000
VERSION     DAFNNB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 49207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49207
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0009"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            559..1368
                     /locus_tag="L3M60_000456"
     CDS             559..1368
                     /locus_tag="L3M60_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6225159.1"
                     /translation="MKDYYKIGEISKIYGIGRDSLMYYEEIGILRPVRDINGYRMYNI
                     SDIWKLNLIKEFRSLNFPMKKIKEYLDDRSIESTKNILNEELNLIDKKVAELISHKEN
                     IIKRLSSIESAVKNTKIDEIEVIYIKKRKALELNADIKRDEDFDFLIQKLQKEYEDRF
                     NILGNNNIGSAFSTEAINKGIFNEFKSVFCFLESNEKVYNIVFDEGYYVTLNYTGSNS
                     NNKVYMEKVFEFIEENNYKVIADPIEIYKIDIHETGIVEEFITEIQVPIIK"
     gene            complement(1477..1686)
                     /locus_tag="L3M60_000457"
     CDS             complement(1477..1686)
                     /locus_tag="L3M60_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225160.1"
                     /translation="MGNNDKEKSKNSKNIFHDIPVLVNNFFWSMIGFIVIKNFGNGNF
                     FTYLLGIGAGLLWGIFIQKFFTNNK"
     gene            complement(2172..2846)
                     /locus_tag="L3M60_000458"
     CDS             complement(2172..2846)
                     /locus_tag="L3M60_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBN6225161.1"
                     /translation="MNHYILIQFEYIARIITAGFCGALIGYERKNRLKEAGIRTHFIV
                     ALASALIIIVSKYGFNDIVGTPGIGLDPARVAAQVVSGIGFLGAGLIFIRNQSVSGLT
                     TAAGIWATAGTGLAIGSGLYLVGIVSSIIIVIVQITLHRDRKWMKLPTAEQVSLKISN
                     KADSIEYIEGKFKKSNIEIINMNIKRLDGDWLEVVIYTKLPKGYKKTNLLKLFSDNPL
                     IETLEI"
     gene            3483..4268
                     /locus_tag="L3M60_000459"
     CDS             3483..4268
                     /locus_tag="L3M60_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6225162.1"
                     /translation="MIVKIDNTLEKEFWQYVSHEESLNLFIIGYVENYGFSSQYQDIW
                     SQVKDGNITSIILKNKSTLIIYSFKNNFDIEEMKNHIKNLDVESISGKKCVIDRLISE
                     YKNFYEKLDNKFCVLKEMKEIDFSNMKGYKIENAQEKDIDEIGKLLNRSDYKVSKNYI
                     EERKEHLKEGNVRAYFIRKNDTMISTVSTGMETSFLAMVVSISTDKRYRGKGLASYMV
                     YNLSKELLLEGKVPCLFYNNDIAGKIYHNIGYKEVNEWTILFK"
     gene            complement(4364..5188)
                     /gene="pdaA"
                     /locus_tag="L3M60_000460"
     CDS             complement(4364..5188)
                     /gene="pdaA"
                     /locus_tag="L3M60_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lactam-biosynthetic de-N-acetylase"
                     /protein_id="HBN6225163.1"
                     /translation="MKKDSLKKYIMIGAFALILFGIASINFKSLDKTKTQISSPTLDT
                     HEYDWYFNPREDGKQPSPIKEADFFKKYDAYYVGNPKEKVIYLSFDAGYESGNTPKLL
                     DTLKKHNAKAQFFVVESYIKSNPDLIKRMEKEGHLVCNHSKSHPSMAGITDFEKFKEE
                     ITSVEKAYKDVTGKEMPKYFRPPMGKFSEQSLKYTQDLGYKSIFWSFAYVDWYEKKQP
                     THEFAKNKIYSRTHPGAIVLLHPNSSTNTEILDEVLTHWEREGYKLKTLDYLSNKK"
     gene            5411..8530
                     /locus_tag="L3M60_000461"
     CDS             5411..8530
                     /locus_tag="L3M60_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HBN6225164.1"
                     /translation="MDNIISVLNDVVFLDIEVSGLDCLNSEILEVGAVKVKDWKIYTY
                     ESLIKNKFEVPIEVFSVCKNLDKNDLEIANEIELVEDRLVNFVEDSFIICHDLSLKKK
                     FFEYYMPKLKNKFIDLIELAVILEPYYKDYSLEYLKNTLTNFNSKVENRALSDAIDII
                     NIVNCLLVRFNSYEKTTLEPLSFKINSYLKKFNLPTWEWSKFLEEANYDLSNNINLKK
                     EYNIFDSKEEKKKERETLKILNEEENHYEELLKYKTIWENKEGFTYEYRPGQYELTKT
                     IRELFRNSEDEEKIACIEAPTGIGKSVGYLLPAILEARINKKRLLISTDTKELQIQLI
                     NKDIPNVLDSLGLNGKVSYGYIKGKNNYICIDRLEAYIDDYESQNPTKGEILSLIFLE
                     RLVEGGKYGDIEEINYSVFDNFKEISIHLRNVSCDPNMCRPKKCKKDCLYKNRIEELK
                     EEHITVVNHSLLAKWPYKDEKPLENIIVDEAHNLTEKGYDFFSSIINSKSLRYLLQEI
                     YPYEFIQNSSFIYKKYSRNMRKIKAFDKFYNVLKIEREDKQKIARSINLIIEEIDSIL
                     NFGSCNEYDSVSNYNLRWELNLQIDEILGKLKKDGVDTEISYRAYSEKIKLGCEKIIK
                     NLVLIIITIYRNIDDDSIDKEADIYKFGKAKTRDLEDIKIIFEIFLEYDEKDDYARIV
                     EIDKNYNDFEFRVVPLKIADLFEENILSQLEKGIFLSATLSLSESMSYFKNTLGIDRV
                     KNVEKIIEPIFDYKNRVSVVGFSDICEYRNSEFPNEMSKIISNISQITEGHTLALFNS
                     KDRQEKTYEILKKYLHSFNLEIYADKKGIRHLNDLNRKCVVLGSKGCFEGVDIPGDGL
                     VCVTLDKLPNLNPKDPLYFTIMKKYNIDYYTINYPQMTIKVKQAMGRILRSKYDYGCF
                     VIFDVGTNISVLKRLEKDLHDCKISKVNSNEFYTYIRQHLNKSRSLILKSVIFDTIKA
                     LNVDSKMNNNDVDKDIIKKDINENIRQRAVKGEVYHIDILKKDIKVKYFNKNYLINLD
                     IFMREENKN"
     gene            8547..9146
                     /locus_tag="L3M60_000462"
     CDS             8547..9146
                     /locus_tag="L3M60_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HBN6225165.1"
                     /translation="MAKQKFYAVKKGKNIGIYNTWDECKKQVNGFSGAEYKSFSTFQE
                     AKEYIYGAEKFSFKEDKEFIEAYVDGSYEHSIKMYGSGVVILKNNEVIKTYSEKGKEE
                     TLVGMRNVAGEIEASKIAMQYCIDNNVQNLILYFDYEGIEKWCTGVWKTNKEGTIAYK
                     NFYDSIKNKLNVKFTKVKAHSGNKYNEEADKLAKKAIGV"
     gene            complement(9359..11488)
                     /locus_tag="L3M60_000463"
     CDS             complement(9359..11488)
                     /locus_tag="L3M60_000463"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HBN6225166.1"
                     /translation="MPNLPSLGSKAPDFKANTTNGSIRLSDYKGNWIVLFSHPGDFTP
                     VCTTEFLCFAKYYDEFKKRNTELIGLSVDSNSSHLAWMYNISLLTGVEIPFPIIEDRD
                     MRIAKLYGMISKPMSDTSTVRSVFIIDNNQILRTILYYPLTTGRNIPEILRIVDALQT
                     SDRDNIVTPANWFPGMPVILPYPKNYKELKNRVNSCNKKYSCMDWYLCFVPDNYNAEE
                     VNKKIDNTCSWKKEHTKNIENECNCEHEHHDYMNKALDCKQEHMTDIKDDCNQEKKHT
                     KNTNKTHSSKQNKFKDKSCDEMKFNYDKDESCDKINSSYDKDDSSYEYFYKHNYKNYD
                     YTSEKNTKKIAMKTLKDSKKLVRPQITDPYNPIVENANCPDINPIVAEYVLGNPTNVD
                     AQLLDAVIFAFAEIDQSGNLFIPYPRFLNQLLALKAEKPSLKVIVAIGGWGAEGFSDA
                     ALTPTSRYNFARQVNQMINEYALDGIDIDWEYPGSSASGIVSRPQDRENFTLLLTAIR
                     DVIGDEKWLSVAGTGDRGYINSSAEIDKIAPIIDYFNLMSYDFTAGETGPNARKHQSN
                     LFDSDLSLPGYSVDAMVRNLENAGMPSEKILLGIPFYGRLGATITRTYDELRRDYINK
                     NGYEYRFDNTAQVPFLVKDGEFAMAYDDSLSIFLKTQYVLRNCLGGVFSWTSTYDQAN
                     ILARTMSIGINDPEVLKEELEGIYGQF"
     gene            11754..12002
                     /locus_tag="L3M60_000464"
     CDS             11754..12002
                     /locus_tag="L3M60_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225167.1"
                     /translation="MMICFAMTFALVLINSVMGVHIGLDYYFLMGSVLTVCLTFGIVL
                     CNARKIFDSKTIHKTRMKNVSTNKKQKSHSRDIKRKIS"
     gene            12168..13559
                     /locus_tag="L3M60_000465"
     CDS             12168..13559
                     /locus_tag="L3M60_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HBN6225168.1"
                     /translation="MNFRDLFEKAVDFIDEKRKSIVAISLSTLGVIVLMIVFFLSSNE
                     FSVGKEANELLKIIEKRQYSIAVDYYTSIEKKFSDSKMERFNKSVSKKINKLLLNSGD
                     KYLDGDISQESFIGLINTVKELKKISIDVDDLLAQADRVREMYKEENTTYDIAINYIN
                     TVSLLNGMGSNLDVYKQNIETIKESRDVYDSAVKDQKVYKYYEAIEKYNKVLKEDEKY
                     YSMAQNGKEQCIEEMYDYYISRAEEANTSGDYERALQYIEYLKEDYSDDEKVQSLEKK
                     YKKNLSLYTLTSDDIINVIAKKSGKDKANLSINSLPQMIKNNKYYYAEVYEYDKLINE
                     VLVSAKTKDLYSYKDGKKDYKVDYGDGYFRILEDGSYQFGITKDKAKFVLTNALDEKE
                     NKYKKIEILDIEKADRYSKSKKSLEELFGKYKNIYYYAVVNKGLFRGKEVYAINIYNE
                     KIYAVSENGLNEY"
     gene            13674..15128
                     /locus_tag="L3M60_000466"
     CDS             13674..15128
                     /locus_tag="L3M60_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBN6225169.1"
                     /translation="MSNMNKKLVIAIGAVALVAIIFIGIMCMQFKGEKIAKNTYVNGV
                     NIGKLTKSQAKQELAKKYKLENVEFNYNDKSWKVKSKDLNLSTDLDKTVENAYNLNRK
                     SGFFGNLSKTISANFGKKSNLVVVVNYDKNKLKAEMGKIAKEIDVDVKDATLDISGEK
                     VKVIPDSDGLKMDVSKSMETFDNQTKKGNYKNKLAVKATPAKVKKEQLANIDTNLGKY
                     STTFKTSQINRSINIKLATDSISNILLMPGETFSFNEHTGKRSKENGYKSAPVIMEGE
                     MEEDYGGGVCQVSSTLYNSVLYAGLEIANVKNHTIPSSYVPKGRDATVADSGIDFLFK
                     NNLKHPVYIKNYVSGNQIVCNIYGSAEDKQNITISTKLDGVSQTTMKRVNDPTMPKGK
                     EKVDKSGRNAYSVSTYRTFNDANGKKIKTEKIANSYYPKKEGIILVGTMEPKPEEKPS
                     TDENKNNQNTNNQQKPETPPTDNKPNETPPQPQD"
     gene            15193..15786
                     /locus_tag="L3M60_000467"
     CDS             15193..15786
                     /locus_tag="L3M60_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HBN6225170.1"
                     /translation="MNLNNITNYVQSICENISNVLDVDVTLVTKDLTRIAGTGIFRGK
                     IGETISEKSVYSLVLKDGKSYVINKEIEENCNKCANRENCKELADICTPVKMGEHILG
                     ILGIAAFNEEQKEKILSKDKELTAFVRSMSDLISFKLDELYKQEVKTIDDLEREAIEN
                     AIKKYGSNTEGMKKVAEALNIGIATLYRKVKKFNIKS"
     gene            15968..17314
                     /locus_tag="L3M60_000468"
     CDS             15968..17314
                     /locus_tag="L3M60_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBN6225171.1"
                     /translation="MPRKILDISLIEDNKSDLTFFNIEEEDGDETLKALKQSYVTTNY
                     KEETLLNLQGALNSKSDTTSKELKLESYKRYNNALDLAYKNYITSAINMVNKALEINP
                     KDIDILNLRGLLKLLKCDFAKAFESFYTALCYENNHISRKYVNLISSEDFKTFLGRYN
                     HSIRFINEELNYESIHILENIIEEEPELIEPYVILSLLYDKLGNVKKREGYLDKLKEL
                     DKDNPVFEKNEEEKEDISKNEETKKVKRKKNILPYIIVGCVLIGMGLYLIQSKKRIEN
                     LNNQISSKEEKLSETDKKLGETSKKLEKTNKELNEVKNKEPERKITVADEEDLYYQAL
                     DLKKKKEYEKAIENFKSIVSSGKTKKYISESIYQLAITNKLLGNKDEAIKYYKKYINT
                     YTKNDQYYDDSYYELGMLYYDNGDLKNAQQTFYSLRSEVPDSMYNNSKIKEILSEK"
     gene            17496..17867
                     /locus_tag="L3M60_000469"
     CDS             17496..17867
                     /locus_tag="L3M60_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBN6225172.1"
                     /translation="MKVFNQLVIILSIWVIGEYISSFIQGIILIPGSIVGMLLLFLLL
                     QFKVLDLSSIENVSGFFLDNMAIFFIPAGVSLIKSLDLIRENVLVLLLVICLSTLIVM
                     YTTGIIVEKMIKKKEEGQKNV"
     gene            17860..18549
                     /locus_tag="L3M60_000470"
     CDS             17860..18549
                     /locus_tag="L3M60_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HBN6225173.1"
                     /translation="MYNVLHTPVFGIIVTIVFFNLGRYIQRKTSNPICNPLLIAIIGI
                     ILFLSISKIPYENYKIGADSLNFFLGPVTVVLAVPLYKQFELFKKHMFEIIAGIGCGI
                     VISFVSVLIIGKIANSDVSIINSLIPKSITTPMGISLANSLNGIESITVVAIIFTGIF
                     GGMIASTVFKLGNINHPVAKGIALGTSAHALGTTKAFEFGEIEGAMSGVSIGVSGTIT
                     VILIPIIMNFI"
     gene            19069..19935
                     /locus_tag="L3M60_000471"
     CDS             19069..19935
                     /locus_tag="L3M60_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBN6225174.1"
                     /translation="MSEVKNTLQKHPVLRYSTMLFGMLMTSIGINGFLRPAHLLSGGA
                     TGIATSINYLTNINVGLLTFLINIPIFILGFIYLEKEFCISSLVNMIVFSLLLGATQE
                     ISKIIPIHDILLQSVYGGILSGLGVGIVFRTRSSQGGTDIIAAILKIKKNIEMKDTAL
                     AINGLIVLTGSFLFGLDLALYTLIGLFLNAYSMSFIKDAMNYQKSVMVMSDEVDLIAE
                     DIMKSLVRGVTFLDAEGAYTHQKKKIIYTIVSSNEIPKIKDIALKYDKKAFISVNDVT
                     EVKGRGFKAKDL"
     gene            20177..20986
                     /locus_tag="L3M60_000472"
     CDS             20177..20986
                     /locus_tag="L3M60_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225175.1"
                     /translation="MNLSKEYVNLYDNYLKYINEVSKEIRSIKSMKKRVLKRGDFAPE
                     NEVLELEGKTIRDIDDVDTKKPKNKIYKFSNGDVYIGKFLEGKMEGKGSYTFFVDEGT
                     AMEYIGEFKEDKKNGKGNFVFSNGNEYIGYFEEDMMNGIGNMLYNSKDEYIGTWKNGK
                     KDGKGIYKWSDGCIYAGEFKGGKMEGYGICYNSKGEILYEGEWKNNLIHGKGTYIWEK
                     GKKYIGEFMHGKKHGQGTFYLNNELVYEGTWKFDKPSIFDRTLDEIFSLRL"
     gene            21127..22191
                     /locus_tag="L3M60_000473"
     CDS             21127..22191
                     /locus_tag="L3M60_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HBN6225176.1"
                     /translation="MKKLIALVLVILIVLFYSPELLKEGNDLKEKFFQSEIWNEFLDK
                     ININDNSKKDKKEKNQNVQSNNTEGDNLGESDIKNFEKISLEDNLSYVLSSIGKPGRI
                     DTSEYGFDWYVYNQYGKEFAMVGVENDEVVALYSNSINSSENQGIKINQDRQTVRTKI
                     TPLKYKRKGNTRYIINSENQYDIISKEGKYITIFYDIHEKNRVCSYLIIDKSTEDEFK
                     KLYPDDREELKKCFELEVIDLVNSVRNQRGLNSLRYSEQATLSSRKHSEDMRDNNFFD
                     HVNKKNETPFDRMKREGIVYTSAGENIAAGQINAIYAHEAWMNSEGHRKNILGNYNNI
                     GVGVIFGGSYKTYYTQNFYK"
     gene            22219..22938
                     /locus_tag="L3M60_000474"
     CDS             22219..22938
                     /locus_tag="L3M60_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6225177.1"
                     /translation="MTSWKKKTVYKCLIAVVLFCGIILISNFSKVSALMTDTSGNVLI
                     KHGSREKKLIAITFDDGPHPKETSQVLDVLKKYNVKATFFIAGKHAKWYKEPLVRASK
                     EGHEIGNHTFNHPDISNLSSSQIEEEIVKCEDILKEVTGKKPTLFRPPFGSYREKELI
                     EIAKKHDYKVVLWTGVDVKDWKNPGANSIADKIINKVQNGDIILLHDYATNDTVEALD
                     MFIPKMIEKGFKFVTVSELIK"
     gene            22961..23545
                     /locus_tag="L3M60_000475"
     CDS             22961..23545
                     /locus_tag="L3M60_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HBN6225178.1"
                     /translation="MILMIDNYDSFVYNLVQYIEELGETVVVKRNNEIKIRDIEELNP
                     EVIVLSPGPCSPKEAGICIDIVEYFKGKKPILGICLGHQTIGHVFGGDIIKAQQPVHG
                     KVYSISHTNKGVFRGLKNPLNVTRYHSLIIDSNTVPKELEVTATTDKGEIMGIRHKKY
                     LIEGVQFHPEAILSEYGHEMLKNFIIEARERVHM"
     gene            23547..24896
                     /gene="pabB"
                     /locus_tag="L3M60_000476"
     CDS             23547..24896
                     /gene="pabB"
                     /locus_tag="L3M60_000476"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HBN6225179.1"
                     /translation="MIREINTTLNSFEIFTIFRNEHDSFILDSAMDKEKLGRYSFISS
                     QPFKVLKYKDTDENPLEVLKEELHKYRVINDTNLPFVGGAVGYLSYDLGNYIETLPRT
                     AIDDIKMPDMYFGFYNHVIVIDHLVQKTYIATPNIDIKLEEKIMDDIEQRILKEEKKG
                     IDSICYEEKEVTPIRLKSNFTKEEFKNAVQSVREYIRQGDIYQANLTQRFSGETELTS
                     FELYRDLRRFSPAPFGAFLNFEDTHILSNSPERFIRCVNNRIETRPIKGTRPRGKNKE
                     EDLKLQQELRNSEKDRAELLMIVDLERNDIGRISKTGSVKVPELFVIEPYANVNHLVS
                     TVVGELKDGKDATDVIKATFPGGSITGAPKIRAMEIIDELEPTQRNVYTGSIGYIGFN
                     GDMDFNIAIRTIIKNDKKVYFQVGGGMTWDSDPDEEYQETLDKAKSIMKALRGYYEE"
     gene            24886..25629
                     /locus_tag="L3M60_000477"
     CDS             24886..25629
                     /locus_tag="L3M60_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV family protein"
                     /protein_id="HBN6225180.1"
                     /translation="MKNNVGFDSSLSKFGIGLFETIKVKNGLPIDLNIHVERMLSSIN
                     CLDLDINYKKDFLINEIVTYIKKENVIDKALRITVFDEGYNISIRDIPYNQEMYEKGF
                     RLTISPIVRGNSLIHRHKTTNYFENIYTKNIANKTGYNDGIFLNSEGVILECSMSNIF
                     FIKGERVYTPSDRLPILNGIIKKRIIEICDELHIELIENEINISEISSFDFVFVTNSL
                     MGAIKVTEIDKIKFNKENVVFDKIEELLE"
     gene            25663..26364
                     /locus_tag="L3M60_000478"
     CDS             25663..26364
                     /locus_tag="L3M60_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225181.1"
                     /translation="MIGCIYEVYNEEISKIIENQNTLAVYLVGSSKDVDFTLYDVEIN
                     DIDVFVFVKEGEKQERILFKKKGIEFDINYFSKDGIKKLLSNREYFFVKEMKDAKVLF
                     DRENISHTIKDISRDIYLEGPSKMSLEEKSFIKQDIGAKISNLKNKEKFDVFEYHFLT
                     NLYLKDIIIGYFNINDKWVPKDKKLLKVLKKENNELFELVSKVSENYDYQRLLDVYNY
                     IFKEIETKEVIKLIF"
     gene            26390..26962
                     /gene="folE"
                     /locus_tag="L3M60_000479"
     CDS             26390..26962
                     /gene="folE"
                     /locus_tag="L3M60_000479"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBN6225182.1"
                     /translation="MNKVDKEKIQHAVREILEAIGEDPDREGLIETPNRVARMYEEIF
                     SGLNEEPRDHLKVLFADEKHEELVLVKDIPFYSCCEHHLVPFFGKAHIAYLPKGGRLT
                     GLSKLARVIDTLAKRPQLQERITKNAADIIMEELQPYGVLVVVEAEHMCMTMRGVKKP
                     GSKTVTSAVRGIFEKDIASRAEAMSLITMK"
     gene            27028..27819
                     /gene="folP"
                     /locus_tag="L3M60_000480"
     CDS             27028..27819
                     /gene="folP"
                     /locus_tag="L3M60_000480"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBN6225183.1"
                     /translation="MFDYGKRTYIMGILNVTPDSFSDGGDFNNLDIAIQHAKDMVDQG
                     ADIIDLGGESTRPGHSYVDSDEELRRVIPVIKKLKQELDIPISIDTYKADVAEEALKL
                     GVTMVNDVWGLRKDKNMASVIGKYDAEVCIMHNQDGTNYDKDIMESIKDFFKISIEMA
                     ISCGVKKEKIVLDPGIGFGKDFEQNIEVLRRLNELKDLGYPILLGTSRKSVIGKVLPV
                     EPKKRLEGTIATTVLGIRDGVDIVRVHDVYENLMAARMTDAIYRK"
     gene            27830..28195
                     /gene="folB"
                     /locus_tag="L3M60_000481"
     CDS             27830..28195
                     /gene="folB"
                     /locus_tag="L3M60_000481"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HBN6225184.1"
                     /translation="MDKILLSNLGFYGYHGVLKEENFLGQKFFVDMELYIDSREAGLS
                     DDINKSVSYAEVYDVVKDITENKQFNLLEALAENIAEEVLNKFILINGVMVRVRKPEA
                     PVNGIYDYFGVEIRRTRDE"
     gene            28188..28694
                     /gene="folK"
                     /locus_tag="L3M60_000482"
     CDS             28188..28694
                     /gene="folK"
                     /locus_tag="L3M60_000482"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBN6225185.1"
                     /translation="MNKAYLGIGTNMGDRFDNLSRACELLKNSDSIYKVKESSLYETK
                     PWGYTEQADFLNMCVEIETEFEPYELLEYCQEIERKLHRERIVHWGPRTIDVDILFFN
                     DVVSTDERLIIPHPRIQDRAFVLIPLMDLNEELIINEKTIKEHLNLLSAEERDEVKEL
                     VGYERKPI"
     gene            28675..29460
                     /gene="aroF"
                     /locus_tag="L3M60_000483"
     CDS             28675..29460
                     /gene="aroF"
                     /locus_tag="L3M60_000483"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBN6225186.1"
                     /translation="MKENLFNNNSKIEINTNNKKITVKKDKLIIAGPCAIESYEQLLE
                     TAKFVKSQGANILRGGAYKPRTSPNSFQGLKKEGLEILKAVKDEVGMAVITELMDVRD
                     MDELYSISDIIQIGSRNMQNFTLLSEVGKQNKPVMLKRGIASTITEWIGASEYIAIEG
                     NSNIIMCERGIRTYNDYTRNTLDLAAVPIIQKETGLPVVVDPSHATGVRYLVKPMSLA
                     SFACGADGIMVEVHPDPENALSDGAQSLCFNEFEDLMKSINNY"
     gene            29715..31505
                     /locus_tag="L3M60_000484"
     CDS             29715..31505
                     /locus_tag="L3M60_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBN6225187.1"
                     /translation="MNDIKDIIEEIKARCDIASIISEYMSIKQSGANYKGLCPFHGEK
                     TPSFYINTSKQIYKCFGCGEGGDIINFVMKMENLDFMDAVKILANKCGIEINTNMNEE
                     TRIKIEKSKKFQDIHTEAARFYLSNLLGSKNLGYEYLRIRGLDDKIIKKFGLGFSLDS
                     WNSLMNTLISKGYKKQDLLECGLIAKNRDGTNCYDKFRNRVMFPIFDYRGNIIGFGGR
                     VLDDSLPKYLNSPDTLIFNKKQNLYGLNFARKNLESKTIVLVEGYMDLISLYQYGIKN
                     VVATLGTALTEQQGLLIKRYADTAIISYDSDEAGIKATLRAIDILTKLGINVKVLDLK
                     DAKDPDEFVRKYGLSDYKKAMDVSTHYIKYKIDHLKKEFNIQKDEERVKFAKEASKII
                     KQLTSPVEIDFYTKYLSNQIDINVESIKREVYGKNYNKPYNNKNQKKIEEKVIEKVEV
                     RQDGKQLVEETLIKIMLEDKKIREIALLKVEESDFLLKESKEILNYMIKNQELDKITI
                     DKLKSLNISEEYLKELNSISLNSINLENTKEIEGIITNIRKNSLEEQINSLLREQQEL
                     ENNNDMKEVDGRVMEIALKIVEINKILKSL"
     gene            31523..32689
                     /gene="rpoD"
                     /locus_tag="L3M60_000485"
     CDS             31523..32689
                     /gene="rpoD"
                     /locus_tag="L3M60_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBN6225188.1"
                     /translation="MENKSNKKELKKVTAKTLIEKGKKQGSLTLAEIMEAFSETELDK
                     DQVENLYETLGNLGIEITETKNYKADIDFSVADDDLSIGHLDEDAEAISHEDSSAIEI
                     ETVDLSLPKGISIDDPVRMYLKEIGKIPLLKPHEEVEFARRMHEGDEIAKQRLVEANL
                     RLVVSIAKRYVGRGMLFLDLIQEGNLGLIKAVEKFDYTKGYKFSTYATWWIRQAITRA
                     IADQARTIRIPVHMVETINKLIRVSRQLLQELGRDPKPEEIAKEMEMTEDKVREIMKI
                     AQDPVSLETPIGEEEDSHLGDFIPDDDAPAPAEAAAYSLLKEQIEDVLGSLNDREQKV
                     LKLRFGLEDGRARTLEEVGKEFDVTRERIRQIEAKALRKLRHPSRSKKLRDYLD"
     gene            32851..33540
                     /locus_tag="L3M60_000486"
     CDS             32851..33540
                     /locus_tag="L3M60_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBN6225189.1"
                     /translation="MKLTDRLLKIASLVSDGKKIADIGTDHGYIPVYLLKEGRVPFAV
                     LADVNKGPLDNAHKEVIQNNLLDKVDLRLGSGIEILEIGEVEEVIIAGMGGILISELL
                     EAKKEVAHNVEKLILQPMQAQEELRYYLLNNGYEILEEVLVREDFRIYEIIVAKYTGK
                     NTIIEDEIYYEVGIKLLENKDSLFKDFIEKKIKTYSSIVSKLEGKNGEAIDKKRNESE
                     VTIKKLENLIK"
     gene            33554..34651
                     /locus_tag="L3M60_000487"
     CDS             33554..34651
                     /locus_tag="L3M60_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBN6225190.1"
                     /translation="MLLKSLTRKIEKKYPLNLAEDWDNVGLIVGDFDMDVKKVLVSLE
                     ANEDVIDEAISKNIDLIVTHHPFIFGKINKINSADLKGRLIQKLIKNNVSLYSMHTNF
                     DIAFDGLNDYFMEIMEFGNSKVLDVTKSENLYKLAVYVPHNYSDELRKVLSNSGAGHI
                     GNYSDCTFSIEGEGQFKPLEGSNPFLGSVNDIETVNEVKIETVVPQKLLGGVISSMLD
                     AHPYEEVAYDLYKLENKGEIFGLGRISKLDKSMTLESLSKKIKEKLNMKHIRVVGNLS
                     TNITKVAVVTGAGSEFVKKAKRQGAEVLITGDVKYHEAQDALDIGMCIVDCGHFDTED
                     IFKNVMKRFLDEISEIEVIKSSVYLNPFSTI"
     gene            34713..35285
                     /locus_tag="L3M60_000488"
     CDS             34713..35285
                     /locus_tag="L3M60_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBN6225191.1"
                     /translation="MKAAVIFHSVCGSTYLLAREYKEALKEMNIEVDIFRVSDEVAKT
                     LPQYYLINSKEYKDEFESINVIKSGRELLDYDAIFMGSPTYYGNVSGPMKMFMDSFSD
                     IWVGAPLSGKIFGCFATAGSQHGGGELALQAMNIFAQHMGMTLLSVPCSIRGGHPAYG
                     ILHIAGDNSNIRPSDDVKIGIRDYLERLSI"
     gene            complement(35345..35812)
                     /locus_tag="L3M60_000489"
     CDS             complement(35345..35812)
                     /locus_tag="L3M60_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBN6225192.1"
                     /translation="MFKEMRLKKREMTKEDTIEVLKNGEFGTFSTISENGYPYGVAVN
                     YVYFDDSIYFHSARNGHKLDNILKNNKVSFLVVANENVIPDKFSTTYSSAIVFGKACT
                     VENEEKKNALVEIIKKYSKGFFEEGMKYIEKDVNLTTVVKIEIDHISGKASRL"
     gene            complement(36198..36674)
                     /locus_tag="L3M60_000490"
     CDS             complement(36198..36674)
                     /locus_tag="L3M60_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBN6225193.1"
                     /translation="MLSKAEIKYFNECASEILSSEKVQLMRTFPHHGNVSCLEHSLSV
                     AYYSYLLCKKLHLNVDIQSVIRGALLHDFFLYDWHYKGDRKGLHGFTHPREALKNATL
                     FFQINEKETDIILKHMWPLTVKPPRYKEAFIVCLLDKFCCLVETLKIHSLLSPYHV"
     gene            complement(36791..37882)
                     /locus_tag="L3M60_000491"
     CDS             complement(36791..37882)
                     /locus_tag="L3M60_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HBN6225194.1"
                     /translation="MSKIECLFTIFPILILMYATFYFMPYFVGKKHIYGVSIDQEYKN
                     YNHFIKLDKKFKNLLSLGFIIDLILVFILVFTFNKLELSYFISIIVFLLYESILYIHT
                     HKKAKNLKSELYSKLGHIDVDSKLIIDMDFINKKNKIIKKFKIIYLIPILFTFGMSIF
                     IVFNYNQLPDSIATHWNINGSPDVFIDKSFLNVFKLIGTNFCLMVLLYITSIGSIKSR
                     IKIDTNRIKESRIENIEYLNKIGYLFLILMTIMTIQFFTTILSIKTKLSTAMNITTLL
                     VIIYLIATYINSPNLKSNSSYSPEENEKYWIAGIMYNNPNDPSLMVNKRFGIGWTINF
                     GNPLGKILYIAIALLLIFLLFSLIKSLLL"
     gene            complement(37875..38264)
                     /locus_tag="L3M60_000492"
     CDS             complement(37875..38264)
                     /locus_tag="L3M60_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6225195.1"
                     /translation="MVLNLDFNSDKSIYVQIKEEITKAIASGELKINESLPSVRSMAE
                     NIGVNLHTVNKSYNELKEEGFLNIDRRKGAIVAQLPMKIDNTTRQKNKLDLELLVAKS
                     YLSGISKEEFLSLSSSLFDNYEVKYYE"
     gene            38456..38902
                     /locus_tag="L3M60_000493"
     CDS             38456..38902
                     /locus_tag="L3M60_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225196.1"
                     /translation="MLTYLMKRQMHKKDDGMSKPMMLTGAVLTGVGAYAVYKMIKNRK
                     SNSQMVNTNYYGNSDYDDYKYDEFDYDCDCRDTKQDYEYEELKKKVKEFNSRRINCEN
                     CPHVSQEEMMQYVNSVKDVKDDVDLHEDAKKDNIYKEDEYKKYEEE"
     gene            39169..39873
                     /locus_tag="L3M60_000494"
     CDS             39169..39873
                     /locus_tag="L3M60_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225197.1"
                     /translation="MVKLLIVEDDSTISFGIKYALEQEGFSIDISKDLSSGKEMISSN
                     EYSLILLDVTLPDGTGYELCQYIRGFSQIPIIFLTACDEEVNIVMGLDIGGDDYITKP
                     FRIRELISRIKAVLRRKANTVEESKKILKFGDLSIYTLEARVYKNDKEVFLTSVEYKL
                     LLILIQNKNTVLSRTQILEKLWDVTYDFVNDNTLTVYIKRLREKIGDDLCEPIYIETV
                     RGLGYKWIGSENSVSI"
     gene            39851..41104
                     /locus_tag="L3M60_000495"
     CDS             39851..41104
                     /locus_tag="L3M60_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225198.1"
                     /translation="MKIVFLYNPEVKKFLSKYVTLIFVIIIISIGFSVINVSLTKDMI
                     VRNNQAIIGTLSSKYPNLESEIVDIITQGKSMENTDYGKKILSKYNYDKSIRINSEPI
                     ISKLVLDTIKINIILVCIIFILIFVLVVRYFKSIYNDLSDMTKYVYYSSEGKSFDMKN
                     KNQEGQIGLLKTELLKMTTILNEKVELLKTEKIFLNNTISDISHQLKTPMTSLIMLND
                     LLYNDIPYEVKIDFLNKIKNQLNRMDWLIKSMLKLSKVEAKVINFKKDKVKFSELIHR
                     AMQSMKIPMEIKNQKLTIEGSDNISYIGDIDWSVEALVNIIKNCVEHTPEFGNITITY
                     KENPLFSELIIKDDGEGIHKKDIPHVFKRFYRGRSSSKEDSVGIGLAMSKSIIESQNG
                     DIYVNSEKGKGTEFHIIFHKMYDSD"
     gene            41184..41861
                     /locus_tag="L3M60_000496"
     CDS             41184..41861
                     /locus_tag="L3M60_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225199.1"
                     /translation="MEVLRVENLTKSYGKNETKVDAIKNVSLSVEKGTFIAITGPSGS
                     GKSTLLHLLGGVDRPTSGKVYINDVDIYNLKTKDLAIFRRRNIGLIYQFYNLIPVLNV
                     KENILLPAELDNRDIDREYFDDLMKTLGLNDREKHLPNQLSGGQQQRTSIGRALINRP
                     SIILADEPTGNLDSKNSKEILELLKLSVKKYNQTLIMITHDKNMALQADRIISIEDGI
                     IKSDEVM"
     gene            41862..44423
                     /locus_tag="L3M60_000497"
     CDS             41862..44423
                     /locus_tag="L3M60_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225200.1"
                     /translation="MNLYTSLTIRYLKQNKRRTIVTIIGIILSTALICGIGNIFESFM
                     DYQVRETIKNDGSFHVTFYDVNRKNVEYVTKSAEVEKYAFTKQLGYSKLENSENGIVS
                     IKQYDKNAINGYKISIKEGRFPAKEGEIVLSENIINLIDDNLKIGDKITLKVGDMFDS
                     SKKKIDSMVFHEGDYIASEKDRTFEVVGIIKKPGFERYNGIATAITYFNPMDNYNDTI
                     NVSVEVRNPKEVYEVSNKISKNIYSNKDKEAVSDMIKYNEHLLRLQGASKYADINSSI
                     KSIIIAVTILVIICTIATVYNSFSISITERKKQFGILNSIGATSSQIKRLVFIEGIII
                     SLIGIPIGLISGTVAIDLLFKIINKYFTESVVTQMSLRIVYNPIIIIVSIIIVLFTIF
                     ISILLPAISASNISPLNVIKNTGEYKVRKIKSSRLIKMIFKTEGVLAYKNIRRNRKKF
                     IVTLFSLMISVIIFISFSGFTLLLLRSEEIRNSQRKYDLYLTAKGTARSIDDTVSELE
                     GIDGIKNFELATGQYVSIRVSENKINKSKEDLIGKYYQKYKVGDSYEYDFSNNDLIFP
                     GDFAIRNKINNLIQGSFNKERAIQENGVILVRKSSFESKGKKGVVELTNYKVGDTVTC
                     EYLDENASSKKIKVKILAITDEERLGMGYQNMGLQFITYDEVAKNLNLKLSKSLIFID
                     SGGSIKTKKEIEALANKNNFNFYDESAITESEKQNLKVIKIFVYGFIAVISLVSVTNI
                     LNTVSTSINLRKGELAVIQSIGVTPKGFRKMIYLESFIYGILSLLFGIPISIGMTLIM
                     NKLISGVIEFSPIIPWTAIVICIVSVFIITFIAGYIPISKLNKENIIDNIRRESI"
     gene            44643..45917
                     /locus_tag="L3M60_000498"
     CDS             44643..45917
                     /locus_tag="L3M60_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6225201.1"
                     /translation="MITISHITAKNMYKSLEERINKFPQGAPPSDTLYKILNVLYTEQ
                     EAKLVAQLPIKPFRVKTAAKIWSVSESEAYRVLDKLASKALILDIEDNKGKKYIMPPP
                     MAGFFEFAMMRTRHDIDQKLLAELYYQYMNVEEDFIKDLFYSTETKLGRVYVQEEVLT
                     NDNEVSILDYERATHIIDESTHIGISMCYCRHRMQHVGKACDAPMDICMTFDNVANSL
                     INNKFARRVDKVECKELLHQAYEHNLVQCGENVRKGVTFICNCCGCCCEAMVAAKRFG
                     NLHPVQTTSFIPNINHESCVKCGKCIAACPIDAISKVKEDGKEYIKIDEDRCLGCGVC
                     VRNCHKNSIMLLKRDEKIITPANSVHRAVLMAIEKGQLQNLIFDNNALASHRAMGAIL
                     SAILKLEPAKKILASKQLKSVYLDKLLSMNDK"
     gene            complement(46079..49120)
                     /locus_tag="L3M60_000499"
     CDS             complement(46079..49120)
                     /locus_tag="L3M60_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp20"
                     /protein_id="HBN6225202.1"
                     /translation="MFKRKLSKLLSSTLVLSMLFTAAPNITFADNTKDNSEKYQSSDI
                     ELHDYSKNSESYTKTKALAKEKIQTLLSKYGVVNAQYALIDNGKIEISGNGGVYSKQD
                     NKNLTKDNMYSIASISKMFTTTAVMKLVDDGKVNLDTPVVNYIPEFKMADNRYKEITP
                     RMLLNHSSGLMGSSFKNTLLLGDNDSYGHDNFLKELQKQRLKAKPGAFSVYCNDGFTL
                     AEILVERVSGMSFTNFLDKYINNPLNLQNTKTTENSFDSSKLAKAYVPYWEDAVPQDN
                     LNAIGAGGLYSSAENLCTFAQTFMKNSNGILSPASVKAMENKEYLNGLWPEGEDSILG
                     YGLGWDCVNTYPFNQYNLKALTKGGDSLLFHSNLIVLPDENMAVAVLSSGGNSQFDEI
                     IGQEILLSALKEKGKIKEIKPDKTFSKPQQVKMPSHLKENSGLYASSNMVKVDVNDNG
                     TLTVSSPYIENGPEDKYIYIGQDRFVSEKGNSCLKFVKEKNNITYLNMSSYDDVPGLG
                     QTASLYYVAQKVDDNNISNSVKEVWKKRNGKGYYLVDEKYTSQSYMFGSVKATPGLSD
                     ETPGYIVNTKIIDENNSNAFIEIPGVIGRDLSDIKLYKENGTEYLSFATQTYVSEDSI
                     TNLPAEKSFTCELASNGYAKWYKIGDDIANKKIEVNLPQNSSFAVYDDKGVPVNYSLV
                     TKNNRVRLPKGGVIVFLGSPNARFEVTYQDEVNASALTGTDRYETSIKISQAGWENAE
                     NAVLINDSAIADALAATPFAYKKNAPILLTGSSQINEKTLAELKRLKVKNVYVVGGEA
                     SINENSLDTIKSNNISVSRISGSDRYQTSMNIAKELNNISNISKISVVNGEKGLADAV
                     SIGAVSAQNDMPIILTNENSNITEINNLFKNKKIDKSYVIGGEYTVSKNIESKLQNPQ
                     RISGSTRNETNAKVIKEFYKDSKIDNLYVAKNGMNKQDDLIDGLSVGVLAGKTKSPVI
                     LVGNSLDYNQKELFKTMRFKSITQIGGNGNENSFKQIKEIA"
BASE COUNT        19739 a         5158 c         8150 g        16160 t
ORIGIN      
        1 ctttcctttt tgaatgcaat tatatcatat tttttcataa atagatagat tgagagatgc
       61 atagttagta atgtagagtt ttttataatt aattttaagt ttaatgattt ttctaatata
      121 taattatttt attcataatt aaccttttat acataattta atatcatatt aataattttg
      181 cataattaat tttagttatt aaaattaagt ttatttttat agtttttgtt aaagattata
      241 ataaatattc atttactaaa tttataaatt attaatctat ggaaatcagt tttaacatga
      301 aaaatattat ataaaaatag atataataac tgtgaacata aaaatatata aaactaaaat
      361 atctttataa aaaagttgaa gttataaata ctatacctaa cattacaata taaaaatatt
      421 ttaataatta attgtaggat gtttattttc ctatccttgt tttagagatt tttttagtat
      481 attctaatac tatagtatta tagagtaata atgttaattt aaaataaaaa tagggttatg
      541 gaggagttta ttatataaat gaaagattac tataaaatag gagagatatc taaaatatat
      601 ggaataggca gagattcatt aatgtattat gaggaaatag ggattctaag accagttaga
      661 gatataaatg ggtatagaat gtataatata tcagacatat ggaaactaaa tttaataaaa
      721 gaatttagaa gtttgaactt tccaatgaaa aaaataaaag aatatttaga tgatagaagt
      781 atagagtcta cgaaaaacat cttaaatgag gagcttaatt taattgataa aaaagtagct
      841 gaacttataa gtcataagga aaatattata aaaagattaa gttcgattga aagtgcagtt
      901 aaaaatacaa aaatagatga gatagaggtc atatatataa aaaaaagaaa agctttagaa
      961 ttaaatgctg atataaagag agatgaagat tttgactttt taattcaaaa gcttcagaaa
     1021 gaatatgaag atagatttaa tatacttgga aacaacaata ttggttctgc tttttctact
     1081 gaagcaatta ataaaggtat atttaatgaa tttaaatctg tgttttgttt tctggaaagt
     1141 aatgagaaag tatacaatat agtttttgat gaagggtatt atgtgacttt aaattatact
     1201 ggaagtaatt ctaacaataa ggtatatatg gagaaggtat ttgaatttat agaagaaaat
     1261 aattataaag taattgctga ccctatagaa atatacaaga tagatataca tgaaactgga
     1321 attgtagagg agtttataac tgaaattcaa gttcctataa ttaaatagct agatttcaac
     1381 aaattagatg ataaatagta taaaaaaatg agtttaccac tatattataa aagtagtggt
     1441 acatttttta tttgggattt tttgtttaag atgtgtttac ttattattag taaaaaattt
     1501 ttgtataaat attccccaca ataaacctgc tccaatacct aataaataag taaagaagtt
     1561 tccattccca aaatttttta taacaataaa tcctatcatt gaccaaaaaa aattgtttac
     1621 caatactggg atgtcatgaa atatgttttt actatttttt gatttttctt tgtcattatt
     1681 acccataatg tcatctcctt accataagca ttgaacactg tgcattacct agaaatcatt
     1741 gaaatattta aactaatttt ttagcataag attatttata aattagatta aaataaaaag
     1801 gatatacgtt tttacaacta gataaaataa ctcataatat attttataaa tatcagtaca
     1861 tatagcttta tatcattaaa gttctctagt tttgatgatt tagtttaatt ttatagttta
     1921 atagtacaat agaattattt attgttcaac atatgtattt taaagttgaa aaaatttata
     1981 gatatgaaga ctattgtaca actaaaaatg cactagatgt ctagaactat ggcaactata
     2041 ctgtagtttt ttaataaaat atatatgtga attgataaaa ataagggacg aactttatat
     2101 agaatcatcc cttgatatct gcatttttat tttaattaaa tatattatta tctttaaagt
     2161 tctattttac attatatttc taatgtttct ataagaggat tatcgctaaa taattttaat
     2221 aaatttgttt ttttgtatcc tttaggtaac ttagtatata taactacctc taaccaatct
     2281 ccatcaagac gtttaatatt catgtttata atttcaatat tagatttttt aaatttacct
     2341 tcaatatatt ctatagaatc agctttatta gaaatcttaa gagaaacttg ttctgctgta
     2401 gggagtttca tccatttcct atctctatga agtgttatct gtacaataac aataatgata
     2461 cttgaaacaa taccaactaa gtataatcca gagccaatgg caagacctgt accggctgtt
     2521 gcccatatac cagcagctgt agtaagacca cttactgatt gatttctaat aaatattaat
     2581 ccagcaccta aaaaacctat cccactgaca acttgtgctg caacgcgggc tgggtctaga
     2641 ccaatacctg gagtacccac tatatcatta aatccatact tggaaacaat gattataagt
     2701 gctgaagcta atgccactat aaaatgagtg cgtattccag cttcttttag tctattcttt
     2761 ctctcatatc caattaaagc tccacagaaa ccagcagtaa tgattcttgc tatgtattca
     2821 aattgaatca atatataatg gttcatttgt ctagcacccc tttctatata atattatata
     2881 tactataata ctacattttt tatgtgaata tagtaaagtt taaaatttat ataatagtta
     2941 tatatgaata taaaataaat aaaagtacat tatgtatata aaaagtatct aattttaaat
     3001 tattaaagag taaactaaac ttactttttt aacatattga atgtttttta tttgtaatag
     3061 ctagataaat acgacaaata tatatgaatt gtatataaat tttatgtttt attaagttct
     3121 aaatttcttc tattgtagtt catatttagt acataaataa ttagtaagat ataagtaaat
     3181 aaatatataa catgactata gaaatttaac tttaaaataa cttatgataa tttagaggag
     3241 gaatatatat gaatatatca agaaaagcta tgaaaataat agaattagca caaaaaattg
     3301 caaataagag aggtatatca gtagaagaag cttggaatga agcagtaaca gagtataaaa
     3361 ataaatacga acatattgca taaatattta aaaatttaga atatttggta gaatataatg
     3421 attttaaatg atactataaa ttataaggta tatcattatt aacatagaag gaggatatct
     3481 gcttgattgt aaaaatagat aatactttag aaaaagaatt ttggcaatat gtatcacatg
     3541 aagaaagttt aaatttattc ataattggat acgttgagaa ttacggattt agctctcaat
     3601 atcaagatat atggtctcag gtaaaagatg gaaatataac ttctataata ttaaaaaata
     3661 agtcgacact tataatttat agttttaaaa ataattttga tatagaagaa atgaagaatc
     3721 atataaaaaa tttagatgta gaaagtataa gtggaaaaaa gtgtgtaata gatagactaa
     3781 taagtgaata caaaaatttt tatgaaaaac tagataataa attttgtgta ttaaaagaaa
     3841 tgaaagaaat agatttttcc aatatgaagg ggtataaaat agaaaatgct caggaaaaag
     3901 atattgatga aataggaaaa cttttaaata ggtcagatta taaagtaagt aaaaattata
     3961 tagaagaaag aaaagagcat ttaaaagagg ggaatgtaag agcatatttt attagaaaga
     4021 atgatactat gattagtact gtgtcaactg gtatggaaac tagttttttg gcaatggtgg
     4081 tctctataag tacagataaa agatataggg gaaaaggact tgcaagttac atggtttaca
     4141 atttgagtaa agaattattg ctggaaggaa aagttccatg tcttttttat aacaatgata
     4201 tagcaggtaa aatatatcat aatataggat ataaagaggt aaatgaatgg acaattcttt
     4261 ttaaataact ataaaaaata aaaaactcta tcgagaatta aagtatttat aaatagctat
     4321 attttactat aattcatgat ggagtttttt ttaatttatg atattatttt ttattactta
     4381 aataatcaag tgttttaagt ttataacctt ctctttccca atgtgtaaga acctcatcta
     4441 atatctcagt atttgttgat gaatttggat gcagtaaaac tattgcacca ggatgagttc
     4501 ttgaataaat tttgtttttg gcaaattcat gagtaggttg tttcttttca taccaatcta
     4561 cataagcaaa actccaaaat attgatttat atcctaaatc ttgtgtatat tttaaagatt
     4621 gttcgctaaa tttacccatt ggaggtctaa aatacttagg catttctttt ccagtaacat
     4681 ctttgtatgc tttttctacg cttgtaattt cttctttaaa tttttcaaaa tctgtaatac
     4741 ctgccattga tggatggctt tttgaatgat tacaaactaa atgtccttct ttttccatac
     4801 gctttattaa atctggattg ctttttatat aactttcaac tacgaaaaat tgtgctttag
     4861 cattgtgttt ttttagtgta tctaataatt ttggagtgtt tccactttca tatccagcat
     4921 caaatgatag atatataact ttttctttgg gatttcctac ataataagca tcatattttt
     4981 taaaaaaatc agcttctttt ataggtgatg gctgtttacc atcctctcta gggttaaaat
     5041 accaatcata ttcatgtgtg tcgagtgttg gactagaaat ttgagttttt gttttatcta
     5101 atgatttaaa atttatacta gcaattccaa aaagtattag ggcaaatgct cctatcataa
     5161 tgtatttttt taggctatct tttttcacaa tttcacctct tatttaaaat ttaaagtata
     5221 tttcttataa tatagtttga atatttatag ttaaaaatat tcatatatac aaataataaa
     5281 tgaaagaagt ttactgtaag atatataaag ttactattaa ttatcttaaa taaagtgtat
     5341 ataaatgata aaatataaag agaaatgatt gtaaataagt taggcgggtt taacttgaag
     5401 ggatataaaa atggataata taataagtgt attgaatgat gttgtttttt tagatataga
     5461 agttagtgga ttagattgtt taaattcaga aattttagaa gttggagctg tcaaggttaa
     5521 agactggaaa atatatacct atgaatcatt aataaaaaac aaatttgagg tacctataga
     5581 agtattctca gtatgtaaaa atttagataa aaatgattta gaaatagcta atgaaataga
     5641 actggtggaa gatagacttg taaattttgt agaagatagt ttcattatat gtcatgattt
     5701 aagtctgaaa aaaaagtttt ttgagtatta tatgccaaag ttaaaaaata agtttattga
     5761 tttaattgaa ttagcagtta ttttagaacc atattataaa gattatagtt tagaatactt
     5821 aaaaaatact ttaacaaatt ttaattctaa ggtggaaaat agagctttgt cagatgctat
     5881 tgatataata aatattgtaa attgccttct agtaagattt aatagctatg aaaaaactac
     5941 tttggaacca ttgagtttta aaataaactc atatcttaaa aaatttaatc tcccaacatg
     6001 ggaatggagc aaatttttag aagaggcaaa ttatgattta tcaaataata taaacctcaa
     6061 aaaagaatat aatatatttg actcgaaaga agaaaaaaag aaggaaagag aaaccttaaa
     6121 aattttaaat gaagaagaaa atcattatga agaattatta aaatacaaaa ctatatggga
     6181 gaataaagaa ggatttacat atgaatatag gcctggtcaa tatgagctta ctaaaacaat
     6241 aagagagttg tttagaaata gcgaagatga agaaaaaatt gcatgtatag aagcgccaac
     6301 tggtataggt aaaagtgtag gatatttact gcctgcaata ttagaagcta gaattaataa
     6361 aaaaagatta ttaatttcta ctgatacaaa ggaattgcag atacaattaa taaataaaga
     6421 tattccaaac gtgttagatt ctttagggct aaatggaaaa gtaagttatg gatatataaa
     6481 gggaaaaaat aactatattt gtatagatag actggaagca tatatagatg attatgagtc
     6541 tcaaaatcct actaaaggag aaattttatc tttaatattt ttggagagat tagttgaagg
     6601 tggtaagtat ggagatatag aagaaataaa ttattcggtg tttgacaact tcaaggaaat
     6661 aagtattcac ttaagaaatg taagttgtga cccaaatatg tgtagaccta aaaaatgcaa
     6721 aaaagattgt ctatataaaa acagaataga agaattgaaa gaagaacata taacagtagt
     6781 aaatcattca ctattagcta aatggccgta taaggatgaa aaacctttag aaaatataat
     6841 agtagacgaa gcacataatt tgactgaaaa aggatatgat ttcttttcaa gcattataaa
     6901 ctcaaagtcc ttaaggtatt tattacaaga aatttatcct tacgagttta ttcaaaatag
     6961 tagctttata tataaaaaat atagcagaaa tatgaggaaa ataaaggctt ttgataaatt
     7021 ttataatgta ttaaaaattg aaagggaaga taaacaaaaa attgctagaa gtataaatct
     7081 tattattgaa gagatagatt caatattaaa ttttggtagt tgtaatgaat atgatagtgt
     7141 aagcaattat aatttaagat gggaactgaa tttgcaaata gatgaaatat tgggaaaact
     7201 aaaaaaagat ggtgtagata ctgagataag ttatagagct tatagtgaaa agataaaatt
     7261 gggttgcgaa aaaataataa aaaatttagt tttaataata ataacaattt ataggaatat
     7321 tgatgatgat agtatagata aagaagcaga tatatataaa tttggaaagg caaaaacaag
     7381 agatttagag gatataaaga ttatatttga aatcttttta gagtatgatg aaaaagatga
     7441 ttatgcaagg atagttgaaa ttgataagaa ttataatgac tttgaatttc gagtagtgcc
     7501 attaaaaata gcagatttat ttgaagaaaa tattttatct caattagaga aaggaatatt
     7561 tttatctgca actttgagtt tatctgaaag tatgtcttat tttaaaaata cattaggaat
     7621 agacagagtt aagaatgtgg aaaagattat agaaccaatt tttgattata aaaatagagt
     7681 ctctgtagtt gggttttctg atatttgtga gtacagaaat tctgaatttc caaatgaaat
     7741 gagtaaaatt atatctaata tatcacagat tacagaaggt catacattag ctctttttaa
     7801 tagtaaagat agacaagaaa agacttatga aatattgaaa aaatacttac atagttttaa
     7861 tttagaaatt tatgctgata aaaaaggaat taggcacctt aacgatttaa atagaaagtg
     7921 tgtagtactt ggctcaaaag gatgttttga aggagtagat attccagggg atggtttggt
     7981 ttgtgtaact ttagacaagc ttccgaatct aaatccaaaa gaccctttgt attttactat
     8041 aatgaagaag tataatatag actactatac tataaattat cctcaaatga ccataaaggt
     8101 aaagcaagct atggggagaa ttttaagaag taaatacgac tatggatgtt ttgtcatttt
     8161 tgatgtagga actaatataa gtgtgttaaa gagattagag aaagatttac acgattgtaa
     8221 aatttcaaag gttaatagca atgagtttta tacttatata agacaacatt taaataaatc
     8281 tagaagctta atactaaaat ctgttatatt tgatactata aaagcgttga atgttgattc
     8341 taaaatgaat aacaatgatg ttgataaaga tattataaaa aaagatatta atgaaaatat
     8401 aagacaaaga gctgtaaaag gagaagtata tcatattgat attttgaaaa aagatattaa
     8461 agtaaaatat tttaataaaa attatcttat aaatttagat atatttatga gagaagaaaa
     8521 taaaaattaa gagtggagtg atatatttgg caaaacaaaa gttttatgct gtgaaaaaag
     8581 gtaaaaatat aggtatatat aatacatggg atgagtgcaa aaagcaagta aatggattct
     8641 caggagctga atataagagt ttttctactt ttcaagaagc taaagaatat atttatgggg
     8701 cagaaaaatt tagttttaaa gaagataagg aatttataga agcatatgta gatggaagtt
     8761 atgaacattc aataaagatg tatggttcag gtgttgtgat tctaaaaaat aatgaagtta
     8821 taaaaactta tagtgaaaaa ggaaaagagg aaactctagt aggtatgaga aatgtagctg
     8881 gagagataga agcttctaaa attgcaatgc agtactgtat tgacaataat gttcaaaact
     8941 tgattttata ttttgactat gaaggtattg aaaagtggtg tactggagtt tggaaaacaa
     9001 ataaagaagg aactatagca tataaaaatt tttatgatag cattaagaat aagcttaatg
     9061 taaaatttac aaaggttaaa gctcattctg gaaataagta taatgaagaa gctgataaat
     9121 tagctaaaaa agctattggt gtataataaa aaaatattta tacataatga catgctacaa
     9181 gagtatatct aaacccctaa tttataatta aataaaattt gatttaaaaa gcagattata
     9241 ttatgagaaa ataagtatct aatttataaa caattaacat cagtgttaat tagacacagc
     9301 ttatttaata taataaaatc tgctttttta tataactata aaatcttact acattctatt
     9361 agaattgccc ataaatacct tcaagttctt cttttaatac ctcagggtca tttataccaa
     9421 tagacatggt tctagccaat atatttgctt ggtcataagt tgatgtccat gagaatacac
     9481 cacctaaaca atttctaaga acgtattgag tttttaagaa tatggataaa gaatcatcat
     9541 aggccattgc aaactctcca tccttaacta aaaatgggac ttgagcagta ttgtcaaatc
     9601 tatactcata tccattttta tttatataat ctcttctaag ttcatcataa gttcttgtta
     9661 tagtagcacc taatcttcca taaaatggta tgccaagaag aattttttca gaaggcattc
     9721 cagcattttc aagatttctt accattgcat caacactata tcctggtaag gataaatcag
     9781 aatcaaaaag gtttgattga tgttttctag catttggacc tgtttcacct gctgtaaaat
     9841 cataactcat aagattaaaa taatctatta ttggagctat tttatctatt tcagcacttg
     9901 aatttatata tcctctatct cctgttccag ctacactaag ccatttttca tctcctataa
     9961 catctcttat agctgttaat aaaagtgtaa agttttctct atcttgaggt cttgatacta
    10021 ttccagatgc actacttcca ggatattccc agtctatatc tattccatct aaagcatatt
    10081 catttatcat ctgattaacc tgtcttgcaa aattatatct agatgtaggt gttaatgctg
    10141 catcagagaa tccttcagct ccccaacctc caatagctac aattactttt aagctaggtt
    10201 tttcagcttt aagagcaagt agttggttta aaaacctagg ataaggaata aataaatttc
    10261 cagattggtc tatttcagca aaggcaaata taaccgcatc caacagttga gcatctacat
    10321 tagttggatt tccaagaaca tattctgcta caattggatt tatatctgga cagtttgcat
    10381 tttcaactat tgggttgtat gggtctgtta tttgtggtct aactagtttt tttgaatctt
    10441 ttaaagtttt catagctatt tttttagtat ttttttcact agtataatcg tagtttttat
    10501 aattgtgttt atagaaatat tcataactac tatcatcttt gtcatagcta gaatttattt
    10561 tatcgcaaga ttcatctttg tcatagttaa acttcatttc atcacaagat ttatctttaa
    10621 acttattttg tttggaactg tgagttttgt tagtattttt agtatgtttt ttctcctggt
    10681 tgcaatcatc tttaatatca gtcatgtgtt cttgtttaca gtcaagagct ttgttcatgt
    10741 aatcatgatg ttcatgttca caattacatt cattttcaat gtttttagta tgttcttttt
    10801 tccagctaca agtattatca attttcttgt tcacttcttc agcattataa ttatctggta
    10861 caaaacataa gtaccagtcc atacatgaat atttcttatt acaactgtta actctatttt
    10921 tcaattcctt atagttttta ggataaggta aaatcactgg cattccagga aaccagtttg
    10981 caggagtaac tatattatct ctatcactag tctgaagtgc atctactatt ctaagtattt
    11041 ctggaatatt tcttcctgta gttagtggat aataaagaat tgttcttaga atttgattat
    11101 tatctataat aaatacagag cgaacagttg atgtatcact cattggtttt gatatcatgc
    11161 catataactt ggcaattctc atatctctat cttctataat aggaaatgga atttctacac
    11221 ctgtaagtaa agaaatatta tacatccaag ctaaatgtga gctgttacta tcaacgctta
    11281 gacctattag ttctgtattt ctttttttaa attcgtcata atatttagca aaacataaaa
    11341 attctgtggt acaaactggt gtaaaatcac caggatgtga aaataaaaca atccaattac
    11401 ccttataatc agagagtcta atagatccgt ttgttgtatt ggctttaaaa tcaggagcct
    11461 ttgaccctaa acttggcaaa tttggcatgt aaatcacccc tcaataaatt atcatttatt
    11521 ttattctatt aattaataca tattagtgtt actactttat taaaagttaa gtaatcctta
    11581 tgtcaaaaaa agatatttat taaaaagatt taatattttc ctaatataaa tgacaatagt
    11641 aattgctatt tttttactaa tttggtatag taatggataa ggagtagtgt caattttagt
    11701 gattatacga ggtgagtgga ttgaaaaaaa ttctaagaat tttagttatc tccatgatga
    11761 tatgttttgc aatgactttt gcattagtat taataaattc agttatggga gtccatatag
    11821 gattggatta ttactttttg atgggaagtg tgcttacagt ttgtttaaca tttggaattg
    11881 ttttatgcaa tgctagaaaa atatttgata gcaaaactat acataaaaca agaatgaaaa
    11941 atgtcagtac taataaaaaa caaaagagtc attctagaga tataaaaaga aagatatcat
    12001 aatttatgta aaggaaatat ttatgtataa aataatgatt gataggtaaa ataaaaacac
    12061 gatttaactt gtgtttttat tttttttcaa gaattatgat ataataacca ataagaaatt
    12121 tagtttacca taaaatataa agattattaa atggagaggg aactataatg aattttagag
    12181 atttatttga gaaggctgta gattttattg atgaaaaaag gaaatctata gttgcaattt
    12241 cattatcaac attaggtgta attgttttga tgatagtatt tttcttaagt agtaacgagt
    12301 ttagtgtagg aaaagaagct aatgagctgt taaagattat agaaaaaaga cagtattcta
    12361 tagctgttga ttactacaca agtatagaaa aaaagttttc tgattcaaaa atggaaagat
    12421 ttaataagtc tgtttctaaa aaaataaata aattactatt aaatagtggt gataaatatt
    12481 tagatggaga tataagtcaa gaaagcttta ttggactaat aaatacagtg aaagaattaa
    12541 aaaaaataag tatagatgtg gacgacttat tagctcaagc tgatagagtt agagaaatgt
    12601 ataaagaaga aaataccact tatgatattg ccattaacta cataaacact gtttcgctat
    12661 taaatggtat gggtagtaac ctagatgtat acaaacagaa tatagaaaca attaaagagt
    12721 ctagagatgt ttatgactca gcagtaaaag accaaaaagt atataagtat tatgaagcta
    12781 ttgaaaagta taataaagta ttaaaagaag atgagaagta ttacagtatg gctcaaaatg
    12841 ggaaagaaca atgtattgaa gagatgtatg attactatat aagcagagct gaagaggcaa
    12901 atacttctgg agattatgaa agagcattac aatatattga atatttaaaa gaagattatt
    12961 cagatgatga aaaggtgcag agtttagaaa aaaaatataa aaagaattta tcattgtata
    13021 ctttaacatc tgatgacatt ataaatgtaa tagctaaaaa aagcggtaaa gacaaggcaa
    13081 atttatctat aaactcactt ccacagatga taaaaaacaa taaatactat tatgctgaag
    13141 tatatgagta cgataaatta ataaatgaag ttttggtaag tgcaaaaact aaagatttat
    13201 attcgtataa agatgggaaa aaagattaca aagtagatta tggtgatgga tactttagaa
    13261 tattagaaga tggaagttat caatttggta ttacaaaaga taaagctaaa tttgtgttaa
    13321 ctaatgctct tgatgaaaaa gaaaataagt ataagaagat tgagatatta gatatagaga
    13381 aagctgatag atattcaaaa agtaaaaaga gcttagaaga attgtttgga aaatataaaa
    13441 atatttatta ttatgctgta gtaaataaag gcttatttag aggaaaagaa gtttatgcta
    13501 ttaatattta caatgaaaaa atatatgctg tatcagaaaa tggactaaac gaatattaat
    13561 taataatatt aaattaatat ataattaaaa agtttttggg gttttaaggc gcattatgta
    13621 gtaatataat aatagggata tgtaaataat tttattgtaa ggagaagtat gggatgagta
    13681 atatgaacaa aaagctagtt atagctattg gagctgtggc attagtagca ataattttta
    13741 taggcataat gtgcatgcaa tttaaagggg agaaaatagc aaaaaacaca tatgttaatg
    13801 gagtaaatat tggaaaatta actaaatcgc aagcaaaaca ggaattagct aaaaaataca
    13861 aattagagaa tgttgagttt aattacaatg ataaaagctg gaaagtaaaa agcaaggatt
    13921 taaatttatc tactgacctg gataaaacag tagaaaacgc atataattta aatagaaaga
    13981 gtggtttctt tggtaatcta tcaaaaacta tttcagcaaa ttttggcaag aagagcaact
    14041 tagtagttgt ggtaaattat gataaaaata aattaaaagc agaaatggga aaaatagcta
    14101 aagaaataga tgtagatgta aaagatgcta cattagatat aagtggtgaa aaagttaagg
    14161 ttataccaga ttctgatggt cttaaaatgg atgtttccaa gagtatggag acttttgata
    14221 atcaaactaa aaaaggtaac tataaaaata agttagcagt aaaagctact ccagcaaaag
    14281 taaaaaaaga gcaacttgct aacatagata caaacttagg taagtattca acaacattta
    14341 agacttccca aataaataga agtataaaca taaaattagc aacggatagc ataagtaata
    14401 tattacttat gccaggagaa actttctcat ttaacgagca tacagggaaa agaagtaagg
    14461 aaaatgggta taagagtgca cctgtaataa tggaaggtga gatggaagaa gattatggtg
    14521 gaggagtttg tcaagtttca tctacattat acaattctgt gttatatgca ggtctagaaa
    14581 ttgctaatgt aaaaaatcat actataccat caagctatgt tccaaaagga agagatgcaa
    14641 ctgttgcaga tagtggtata gatttcttgt ttaaaaataa tctaaagcat ccggtttaca
    14701 taaaaaatta cgtttcagga aatcaaattg tttgtaacat atatggaagt gcagaagata
    14761 aacaaaatat aacaatatca acaaaattag atggtgtttc acagactaca atgaaaagag
    14821 ttaatgaccc tacaatgcct aaaggtaaag aaaaagtaga taaaagtgga agaaatgcat
    14881 attctgtatc aacatataga acatttaacg atgcaaatgg taagaaaatt aaaactgaaa
    14941 aaatagctaa ttcatattat cctaaaaagg aaggtattat attagttggt actatggaac
    15001 caaaaccaga ggaaaaacca agtacagacg aaaataagaa taatcaaaat acaaacaatc
    15061 aacaaaaacc agaaacacca ccaactgata ataagcctaa tgagacacca ccacaaccgc
    15121 aagattaaag ttctcaagaa taggatttat gtagagtaat aaaaaatata aatctaaata
    15181 ggagggatta ctttgaattt aaataatata acgaattacg ttcaaagtat ttgcgagaat
    15241 atttctaatg tcttggatgt agatgttact ttggttacaa aagatttaac taggatagca
    15301 ggtacaggaa tattcagggg taaaatagga gagacaatat cagagaagtc agtgtactct
    15361 cttgttttaa aagatggaaa atcctatgtt ataaataagg aaatagaaga gaactgtaat
    15421 aaatgtgcaa atagagaaaa ttgtaaagag ttagctgata tatgtactcc cgttaaaatg
    15481 ggagagcata tacttggaat cttgggtata gctgcgttta atgaagaaca aaaagaaaag
    15541 atactttcaa aagataagga gttaactgct tttgtaagga gtatgtctga tttaatatct
    15601 tttaaactag atgaacttta taaacaagaa gttaaaacta tagatgacct tgagagggaa
    15661 gctatagaaa atgctataaa aaaatatgga agcaacacag aaggaatgaa aaaagtagct
    15721 gaggcattga atataggtat agctacactc tatagaaaag taaaaaaatt caacattaaa
    15781 agttaatagt attgaaaaag aggtaacctt tttatgaaaa gaagaggatt acctttttta
    15841 ttatgaaaat agtggaaaaa tatgtatagt tttgacttga catattaaat atgttatctt
    15901 aaatatatat tttagagtgg aggtgagtat tattttagtt aaagatataa aacaagatat
    15961 agaaagcatg cctagaaaaa ttttagatat atctttgata gaagataata aaagtgattt
    16021 aacttttttt aatatagaag aagaagatgg tgatgaaaca ttaaaagcat taaaacagag
    16081 ttatgtaact acgaattata aagaagaaac attattaaac ttgcaaggag ctttaaattc
    16141 aaagtcagac acaacatcta aggagttaaa attagaatca tataaaagat ataataatgc
    16201 attggattta gcatataaaa attatataac tagtgccatt aatatggtaa ataaagctct
    16261 agagataaat cctaaagaca ttgatatatt aaatcttaga ggtcttttaa agttattaaa
    16321 atgtgatttt gccaaagcat ttgagagttt ttatactgct ttatgttatg aaaataatca
    16381 tatatctaga aagtatgtaa atctaatttc atctgaagat tttaaaactt ttttaggaag
    16441 atacaatcat tctataagat ttataaatga agaattaaat tatgagtcaa tacatattct
    16501 agagaacata attgaagaag aaccagaact aatagaacca tatgtaattt tatctttgct
    16561 atatgataaa ctaggtaatg taaaaaagag agagggatat ttagacaaac ttaaagaatt
    16621 agataaagat aatcctgttt ttgaaaaaaa tgaagaagaa aaagaagaca taagtaaaaa
    16681 tgaagaaaca aaaaaagtaa aaagaaagaa aaatatttta ccatatatta tagttggttg
    16741 tgttttaata ggcatgggtt tatatttaat tcaaagcaaa aaaagaatag aaaatttaaa
    16801 taatcaaata agtagcaaag aagaaaagtt aagtgaaact gataaaaagt taggggaaac
    16861 gagtaaaaaa ttagaaaaaa cgaataagga attaaatgag gttaaaaata aagaaccaga
    16921 aagaaagata actgtagctg atgaggaaga tttatattat caagctctag atttaaagaa
    16981 aaagaaagaa tatgaaaaag ctatagaaaa tttcaaaagt atagtttctt ctggaaaaac
    17041 taaaaaatat atttctgaat caatatatca attagcaata acaaataagt tattgggaaa
    17101 taaggatgaa gctataaaat attacaaaaa atatataaat acttatacta aaaatgacca
    17161 gtattatgat gattcatatt acgagttggg tatgctttat tatgataatg gcgatttaaa
    17221 aaatgcacaa cagacttttt atagtttgag aagtgaagtt ccagacagta tgtataacaa
    17281 ttcaaaaata aaagaaattt taagtgaaaa ataggtttat atagttaagt tttttgaaac
    17341 aaccaagtat gtattttatt aaattaaaat aaaaataaat ttaaaaagag attgtatcaa
    17401 attataactt tctattttaa agaaagatat ttgcttgttg ataaaactac aaatatatga
    17461 tttgaagcaa tctctttagt ttttggaggt ggaaaatgaa agttttcaat caattagtaa
    17521 taattttatc tatctgggta ataggagagt atataagttc ctttatacaa ggaattatac
    17581 ttattcctgg gagtatagtt ggtatgctgc tattatttct actattacaa tttaaagttt
    17641 tagatttgag tagtatagaa aatgtaagtg gatttttcct cgataatatg gctatatttt
    17701 ttattccagc aggtgtatca cttataaagt cattagattt aataagggaa aatgtattag
    17761 tacttttatt agtcatatgt ttaagtacac taatagtaat gtatactact ggaattatag
    17821 tggaaaagat gataaaaaag aaggaagaag gacaaaaaaa tgtataatgt attgcacacg
    17881 ccagtatttg gtataatagt aacaatagta ttttttaatt taggaagata tattcaaaga
    17941 aaaacaagta atcctatatg taatcctcta ttaattgcaa tcataggaat aatattattt
    18001 ttaagtatat ctaaaattcc ttatgagaat tataagatag gggcagatag tctaaatttc
    18061 tttttaggtc ctgtcacagt agtgcttgca gttccgcttt ataaacaatt tgaactattt
    18121 aaaaagcata tgtttgagat cattgcggga ataggttgtg gtatagtgat ttcttttgta
    18181 tctgtattga taattggcaa aatagctaat tcagatgtaa gtataataaa ttcactgatt
    18241 ccaaaatcta taacaacacc aatgggaata tctttagcaa attctttaaa tggtatagaa
    18301 tcaataacag ttgtagccat aatttttaca ggtatttttg gcggtatgat tgcttctaca
    18361 gtattcaaac ttgggaatat aaatcatcca gttgctaaag gaattgctct tggaacgtct
    18421 gcacatgctt taggaaccac aaaagctttt gaatttggag aaatagaggg agctatgagt
    18481 ggtgtttcta ttggagtttc tggtacaatt acagtaatac taattccaat tataatgaat
    18541 tttatataaa aaataaaatt aagataagta tcttgaatac ttaaaacaaa ataaaataaa
    18601 aaaaatttaa aataaaaact gaaagaaaaa aatgcaaata atgaaataaa aaaatcatac
    18661 actgaaatta taaaaacaaa aatttcattt aggaattaat tgtaaagata taattattaa
    18721 attcaaattt gctaaaataa aataaattac tgctaaaatg gatatttaga aaaaaatact
    18781 ataaatttac tatattgcaa gttaaataat aagtagtatc atactatgct agaaaaagtt
    18841 tcatttaaag cataaatgag atgatttgaa cgtttttaaa gattggaata taaaagatta
    18901 cactagacat atatgaaaaa gataatataa tagatgatgt aatagatgac agttcaagaa
    18961 attatataaa aaatttattt gaaatattat ctattacatc aggaataata tatgtaagag
    19021 taagctttta aaattctaag gatttagaaa gattgaaggg agaaagaaat gagtgaagtt
    19081 aagaataccc tacaaaaaca tccagtttta agatattcaa caatgctatt tgggatgttg
    19141 atgacatcta taggaataaa tgggttctta agaccagcac atctacttag tggaggtgct
    19201 acaggtatag caacatctat aaactatttg accaatataa atgtaggttt attaacattt
    19261 ttaatcaata ttccaatatt tatcttaggg tttatatacc tagaaaaaga gttttgtatt
    19321 tcaagtttgg taaatatgat tgtattttca ttattattag gtgcaactca ggaaataagt
    19381 aagattatac ctatacatga tatattgctt caaagtgtat atggaggtat attatctggt
    19441 ttaggggttg gaattgtatt tagaactagg tcttctcaag gtggtacaga tataatagct
    19501 gcaatactaa aaattaagaa gaatatagaa atgaaagaca ctgccttagc aataaatggc
    19561 ttgatagtac ttacaggaag ttttttattt gggttagatt tagcattata cactcttata
    19621 ggtctatttc taaatgcgta ttcaatgagt tttattaaag atgcaatgaa ttaccaaaaa
    19681 tctgttatgg taatgtcaga tgaagtcgat ttaatagcag aagatataat gaagagttta
    19741 gttagaggtg taactttttt agatgcagaa ggtgcatata ctcatcaaaa aaagaagatt
    19801 atatatacaa tagtttcatc aaatgagata ccaaagataa aagacatagc tttgaagtat
    19861 gataaaaagg cttttatatc tgtaaatgat gttacagaag taaaaggaag agggtttaaa
    19921 gcaaaagact tatagaaaat tttaaaagct gggagtttgt aatgtaaatt tgtacattat
    19981 atctctcagc ttatttaaat ttagtatatg aaaataaagg ttttattatt gatttgtgta
    20041 tcactttaaa acatatcatt ttaaaattaa aattgaaaaa atcactcaat atgtagaaaa
    20101 catgaccact attaggatat aataaattta atagatttag taaaatatct aggaataacg
    20161 ttggaggaat ggcattatga atttatctaa agaatatgtt aatttgtatg ataattattt
    20221 gaaatatata aatgaagtaa gcaaagagat tagaagtata aagagtatga aaaaacgtgt
    20281 tttaaaaagg ggagattttg ctccagaaaa tgaagtatta gaattggaag gaaaaacaat
    20341 acgtgatata gatgatgtag atacaaaaaa accgaaaaat aaaatatata aattttcaaa
    20401 tggggatgta tatataggga aattcttaga gggaaagatg gaggggaaag gctcatatac
    20461 tttttttgta gatgaaggaa cggctatgga gtacatagga gaatttaaag aagacaaaaa
    20521 aaacggaaaa ggaaactttg tattttcaaa tggaaatgaa tatataggct attttgagga
    20581 agatatgatg aatggtattg gaaatatgtt atataatagc aaagatgaat atattggaac
    20641 ttggaaaaat ggaaaaaaag atggaaaagg tatttataag tggagtgatg gatgtatata
    20701 tgcaggtgaa tttaaaggtg ggaagatgga aggatatggc atctgttata atagtaaagg
    20761 tgaaatcctt tatgaaggag aatggaagaa taatttgatt catggtaagg gaacttatat
    20821 atgggaaaaa ggtaaaaaat atataggtga atttatgcat ggtaaaaaac atgggcaagg
    20881 aactttttat ctaaataatg aattggttta tgaagggact tggaaatttg ataagccatc
    20941 tatatttgat agaacattag atgaaatatt ttccttgagg ctgtagcttt atctaattag
    21001 ataaagcttt tttctaatac ttactgtaaa taggtatgaa tgatattgat tagtatacac
    21061 tatcaatatt agcctaataa atgtaatatt aaaaaatttt gattgatttt aaaaaggaga
    21121 gatatattga agaaattaat tgctttagtg ttagtaatac ttattgtttt attttatagc
    21181 cctgaattgt taaaagaagg aaatgattta aaagaaaagt tttttcaaag tgaaatatgg
    21241 aatgaatttt tagataaaat aaatataaat gataattcta agaaagataa aaaagaaaaa
    21301 aatcaaaatg tacaatcaaa taatactgaa ggggataatt taggagagtc agacataaag
    21361 aattttgaaa aaataagttt agaagacaat ttaagctatg ttttatcaag tattggtaaa
    21421 cctggtagaa ttgatactag tgaatatggt tttgattggt atgtatataa tcaatatgga
    21481 aaagaatttg caatggttgg agttgaaaat gatgaagtag tagcacttta tagcaattct
    21541 attaattcat ctgaaaatca aggtataaaa ataaatcaag atagacagac agtgagaact
    21601 aagattacac cgttaaagta taagagaaaa ggaaatacca gatatataat aaactcagaa
    21661 aatcaatatg atataataag taaagaaggt aagtacatta caattttcta cgatattcat
    21721 gaaaaaaata gagtttgttc atatttaata attgataaaa gtaccgaaga tgagtttaaa
    21781 aaattatatc cagatgatag agaagagctt aaaaaatgtt ttgaacttga agttatagac
    21841 cttgtaaata gtgttagaaa tcaaagagga ttaaattcac tgagatacag tgaacaggca
    21901 actttgagtt caagaaaaca tagtgaagat atgagagata ataatttttt tgaccatgta
    21961 aacaagaaga atgaaactcc atttgacaga atgaaaagag aagggatagt atatacttca
    22021 gctggagaaa acatagcagc aggacagatt aatgcaatat atgcacatga agcttggatg
    22081 aactctgaag gacatagaaa aaatattttg gggaattata ataatattgg agttggagta
    22141 atatttggag gaagctataa aacatattac actcaaaatt tttataaata actaaaatat
    22201 ttgaaaggaa gtttaaaaat gacatcatgg aaaaaaaaga cagtatataa gtgtttaata
    22261 gcagtagtct tgttttgtgg aataatatta atatctaatt ttagcaaagt ttctgctttg
    22321 atgacggata ctagtggaaa tgttttgatt aaacatggtt caagagaaaa aaagttaata
    22381 gcaattacct ttgatgatgg tcctcatcct aaagaaacta gtcaagtttt agatgtgtta
    22441 aagaaataca atgtaaaagc tacctttttt atagctggaa aacatgcaaa atggtataaa
    22501 gaaccattag taagagcaag taaagaaggt cacgagatag gaaatcatac tttcaatcat
    22561 cctgatataa gtaatttaag ttctagtcag atagaagagg agattgttaa gtgtgaagac
    22621 attttgaaag aagtaactgg aaaaaaacct acattattta gaccaccatt tggaagttat
    22681 agagaaaaag aattaataga aattgcaaag aagcatgact ataaagttgt tttatggaca
    22741 ggcgtagatg tgaaagattg gaaaaatcca ggagctaata gcattgcaga taaaataatt
    22801 aataaagtac aaaatggaga tataatactt ttacatgatt atgcaactaa tgatacagtt
    22861 gaagctttag atatgtttat cccaaaaatg attgaaaaag gcttcaaatt tgtaacagtt
    22921 tctgagttaa taaaataatt tttaggggga gatttacagt atgattctta tgatagacaa
    22981 ttatgattct tttgtataca acctagtaca atatatagaa gaattagggg aaacagttgt
    23041 agtaaaaaga aataatgaaa ttaaaataag ggacattgaa gagctaaatc cagaggtaat
    23101 tgtgttatct ccaggaccat gctctccaaa agaagctggt atttgtatag atattgttga
    23161 gtattttaag gggaaaaaac ctatactagg aatttgttta ggtcaccaaa ctattggaca
    23221 tgtttttgga ggagatatta taaaagctca acagcctgtg catggaaaag tatatagtat
    23281 tagtcatact aataaaggag tttttagggg gcttaaaaac cctttaaatg taactaggta
    23341 tcattcacta ataattgatt ccaatacagt tccaaaagaa ctagaagtta cagccactac
    23401 tgataaaggg gaaataatgg gaattagaca taagaaatat ttaatagaag gagtacagtt
    23461 tcatccagaa gctattttgt cagagtatgg tcatgaaatg cttaaaaact ttataataga
    23521 agctagagaa agggtacata tgtaatatga taagagagat aaatactaca ctaaattctt
    23581 ttgagatatt tactattttt agaaatgaac atgacagttt tatattagac agtgctatgg
    23641 ataaagaaaa attgggaaga tattctttta taagtagcca accattcaag gtattaaaat
    23701 ataaggatac tgatgaaaat cctttagaag ttttaaaaga agaactacat aaatatagag
    23761 ttataaatga cactaatctt ccatttgtag gaggtgctgt gggatattta tcctatgatt
    23821 taggtaatta catagaaact ttacctagaa ctgctataga tgatattaag atgcctgata
    23881 tgtattttgg attttataat catgtaatag ttatagacca tcttgtgcaa aaaacttata
    23941 tagctactcc aaacatagat ataaaattag aagaaaagat aatggatgat atagaacaaa
    24001 gaatattaaa agaagaaaaa aaaggcatag atagtatatg ttatgaagaa aaagaagtaa
    24061 cacctataag acttaaatct aactttacaa aggaagaatt taaaaatgca gttcaaagtg
    24121 ttagagagta cataagacaa ggagatatat atcaagctaa tttaacacaa agattcagtg
    24181 gagaaacaga acttacaagc tttgaattgt atagggatct aagaagattt agcccagctc
    24241 catttggagc attcttaaat tttgaagaca cacatatatt atcaaattct ccagaaagat
    24301 ttataagatg tgttaataat agaatagaaa ctagaccaat aaaaggtact cgtccaagag
    24361 gtaaaaataa agaagaagat ttaaaacttc aacaagaact tagaaatagt gaaaaagaca
    24421 gagctgaact gcttatgata gtagatttag aaagaaatga tattggaaga atatctaaaa
    24481 ctggaagtgt taaagttcca gaactatttg taattgaacc atatgcaaat gtaaatcact
    24541 tagtttccac agttgtaggt gagttaaaag atggcaaaga tgctactgat gtaataaaag
    24601 caacttttcc aggggggtct ataacaggag cacctaagat tagagcaatg gaaattatag
    24661 atgaattaga gccaacacaa agaaatgtat atactggttc aataggttat ataggtttta
    24721 atggtgatat ggattttaat atagcaatta gaacaataat caaaaatgac aaaaaagtct
    24781 attttcaagt tggaggaggt atgacttggg attctgaccc agatgaagaa tatcaagaaa
    24841 cacttgataa agcaaaatct ataatgaagg ctttgagggg atattatgaa gaataatgta
    24901 ggttttgata gtagtttaag taaatttgga attggtttgt ttgaaactat taaagttaaa
    24961 aatggattac caatagattt aaatatccat gtagaaagaa tgctaagttc aataaattgt
    25021 ttggatttag atataaatta taaaaaagat ttcttgataa atgaaattgt tacatatata
    25081 aaaaaagaaa atgttattga taaagcactt agaattaccg tttttgatga aggttataat
    25141 atatctatta gggatattcc atataatcaa gagatgtatg aaaaggggtt tagattaact
    25201 atatcaccga tagttagggg gaatagttta attcatagac ataagactac taactatttt
    25261 gaaaatatat ataccaaaaa tatcgcaaat aagactggtt ataatgatgg tatattttta
    25321 aactcagaag gtgtaatatt agaatgttct atgagcaata tcttctttat aaaaggagaa
    25381 agagtgtaca ctcctagtga tagattgcca atattaaatg gaataataaa aaaaaggatt
    25441 atagagattt gtgacgaatt acatatagag ttgatagaaa atgaaattaa tatatcagaa
    25501 attagtagtt ttgattttgt ttttgtcaca aatagtctta tgggcgctat aaaggttaca
    25561 gaaattgaca aaataaaatt taataaagaa aatgttgttt ttgataaaat agaggagtta
    25621 ttagaataaa ttaaaatatt aacaatttgt tggagacaaa agatgatagg ctgtatttat
    25681 gaagtataca atgaagagat aagtaaaata atagaaaacc aaaatacttt ggctgtatat
    25741 ttggtgggtt ctagtaaaga tgtagatttt actctttatg atgtagaaat aaacgatatt
    25801 gatgtattcg tttttgtaaa agaaggggaa aaacaagaga gaatactatt taaaaaaaag
    25861 ggaatagaat ttgatataaa ttacttttca aaagatggta taaaaaaatt attatcaaat
    25921 agggaatact tttttgtgaa agaaatgaaa gatgctaaag tactgtttga tagggaaaat
    25981 atatcacata ctataaaaga tataagtaga gatatttact tagaaggacc tagtaaaatg
    26041 tcattagagg aaaagtcttt tataaagcaa gatattggag caaaaatatc gaatttaaaa
    26101 aataaagaaa aatttgatgt ttttgaatat cattttttga cgaatttata tttaaaagat
    26161 ataataattg ggtactttaa tataaatgat aaatgggtac caaaagataa aaaattgcta
    26221 aaagttttaa aaaaagaaaa taatgagctt tttgaactag tgtcaaaagt aagtgaaaat
    26281 tatgactatc aaagactttt agatgtttat aattatattt ttaaagaaat tgagacaaaa
    26341 gaggttataa aactaatctt ctagtcacaa atcaatgggg ggagtttaaa tgaataaagt
    26401 tgataaagaa aagatacaac atgcagtaag agaaatatta gaggcaattg gggaagaccc
    26461 agatagagaa ggtctaatcg agactccaaa tagagttgct aggatgtatg aagagatatt
    26521 ctctggtctt aatgaagaac caagagacca tttaaaagtt ctttttgcag atgaaaaaca
    26581 tgaagaatta gtgttggtaa aagatattcc attttattcg tgctgtgagc atcatctagt
    26641 tcctttcttt ggaaaggctc atattgctta cttaccaaaa ggaggaagac ttacaggtct
    26701 ttctaaactt gctagagtaa tagatacatt ggcaaaaaga ccgcaattac aagaacgaat
    26761 cactaaaaat gctgcagata taataatgga agaattacaa ccttatggtg tcttagtagt
    26821 agtagaagct gaacatatgt gcatgacaat gagaggtgta aaaaaaccag gttcaaaaac
    26881 tgttacatca gcagtgagag gtatatttga aaaggacatt gcgtctagag ctgaagctat
    26941 gagtttgatt actatgaagt agcaatttat tatatataga atcatttatt gagatatgaa
    27001 atttaattta agggagtgtc attaattgtg tttgattatg gtaaaagaac ttatataatg
    27061 ggaatattaa atgtaactcc agatagtttt tctgatggag gagattttaa taacttagac
    27121 atagcaatac aacatgcaaa agacatggtt gaccaaggtg cagatataat agacttagga
    27181 ggagagtcta cacgtccagg tcatagctat gtggattcag atgaagagtt aagaagagta
    27241 atccctgtaa taaaaaaact taaacaggaa ttggatatac caatatcaat agacacttat
    27301 aaggcagatg ttgcagaaga agccttaaaa ctaggtgtta ctatggtaaa tgatgtttgg
    27361 ggtcttagaa aagataaaaa tatggcttct gtaataggaa aatatgatgc agaagtgtgt
    27421 ataatgcata atcaagatgg gacaaactat gacaaagata taatggagtc aataaaagat
    27481 ttttttaaga taagtataga aatggcaata agttgtggtg taaaaaaaga aaaaatagta
    27541 cttgacccag gtataggatt tggcaaggat tttgaacaaa atattgaagt attaagaaga
    27601 ttaaatgaat taaaagattt agggtatcct attttgcttg ggacatctag aaaatcagta
    27661 ataggaaaag tacttccagt tgagcctaaa aaaaggttag aaggtacaat agctactact
    27721 gttcttggta taagagatgg tgtagatata gttagagtac atgacgttta tgaaaattta
    27781 atggcagcta gaatgactga tgcaatatat agaaagtagg tataatataa tggataaaat
    27841 attacttagt aatttaggat tctatggata tcatggtgtt cttaaagaag aaaattttct
    27901 aggtcagaaa ttctttgttg acatggaact ttatattgat tcaagagaag caggtcttag
    27961 tgacgatata aataaatctg taagctatgc tgaggtttac gatgttgtta aagatattac
    28021 tgaaaataaa caatttaatc ttttggaggc tttggcagaa aatattgctg aagaagtctt
    28081 gaataaattt atacttataa atggagttat ggtcagagtt agaaagcctg aagctcctgt
    28141 aaatggtatt tatgactatt ttggtgttga gataaggaga acaagagatg aataaggctt
    28201 atttgggaat aggtactaac atgggtgata gatttgataa tttatcaaga gcatgtgaac
    28261 ttttgaaaaa cagcgattct atatataagg taaaagaatc aagtttatac gaaacaaaac
    28321 catggggtta tacagaacaa gcagattttc taaatatgtg tgtagaaata gaaacagagt
    28381 ttgagcctta tgagttgtta gaatattgtc aagaaataga gagaaaacta cacagagaaa
    28441 gaatagttca ttggggacca agaactatag atgtagatat actatttttt aatgatgtag
    28501 tttccactga tgaaagacta ataatacctc acccaaggat tcaagataga gctttcgttt
    28561 tgataccact tatggattta aatgaagaac ttataataaa tgaaaaaaca ataaaagaac
    28621 atttaaacct cctatctgct gaagaaagag atgaagtaaa ggagcttgta ggttatgaaa
    28681 gaaaacctat ttaataataa ttcaaagata gagataaaca caaataacaa aaagataact
    28741 gtaaaaaagg ataaacttat aatagctgga ccatgtgcaa tagagagtta tgagcaactt
    28801 ttggagacgg ctaaatttgt aaaaagtcag ggagcaaata ttttaagagg tggagcgtac
    28861 aagccaagaa catcaccaaa ttcatttcaa ggattaaaaa aagaaggtct tgaaatatta
    28921 aaagcggtta aagatgaagt tggtatggct gttattacag aacttatgga tgtaagagat
    28981 atggacgaat tgtatagtat aagtgatata attcaaattg ggtcaagaaa tatgcaaaac
    29041 tttactcttt taagtgaagt gggaaaacaa aataaacctg ttatgttaaa aagaggaata
    29101 gcatccacaa ttactgaatg gataggtgct tctgaatata tagcaataga aggaaatagt
    29161 aatattataa tgtgtgagag aggtataaga acatataatg attatactag aaatacattg
    29221 gatttagctg cagtacccat tattcaaaag gaaacaggtc ttcctgttgt ggtagaccca
    29281 agtcatgcta ctggagttag gtatttggta aaacctatgt cactggcatc ttttgcatgt
    29341 ggagcagatg gaataatggt agaagtgcat cctgacccag aaaatgcttt gtcagatggt
    29401 gctcaatctc tttgttttaa tgaatttgaa gatttaatga aatcaattaa taattattaa
    29461 aagaggcatg agcctctttt ttattcgtaa tcgttattaa ggtaaattta taagttataa
    29521 tttgatggtt atattattta ttataaataa agatttaata ttgatagaat taatataaaa
    29581 aataaaaatt ttttgattaa taagaaggat atcaataaaa aaagtcgaat tgtgtatatt
    29641 gagtgattga caaatttaga atatagtgta gaatagaaat gttaacacaa aaaattacac
    29701 cagaaggtga taatatgaat gatataaaag acattattga agaaataaaa gctagatgcg
    29761 atatagcaag tataatatct gaatatatga gtataaaaca atctggagct aattacaaag
    29821 gattatgccc atttcatggc gaaaagacac catcttttta tataaataca tcaaaacaga
    29881 tttataagtg tttcggttgt ggtgaaggtg gagatataat aaattttgtc atgaaaatgg
    29941 aaaatttaga ttttatggat gctgtaaaaa ttttagcaaa taaatgcggt atagaaatta
    30001 acactaacat gaatgaagaa actagaatta aaatagaaaa atctaagaaa tttcaagata
    30061 ttcacacaga agcagctaga ttttatcttt caaacttatt gggaagcaaa aatcttgggt
    30121 atgaatattt aagaataaga ggactagatg ataaaattat taagaagttt ggtttaggat
    30181 tttctttaga ttcatggaat tcacttatga atactctaat cagtaaagga tataaaaaac
    30241 aagacttatt ggagtgcggt ctaattgcaa aaaatagaga tggaactaat tgctatgata
    30301 aatttaggaa tagagttatg tttcctatat tcgattatag aggaaacata atagggtttg
    30361 ggggaagagt attagacgat tctttgccaa aatacttaaa ttctccagac acattaattt
    30421 ttaataaaaa acagaactta tacggattaa attttgctag aaaaaattta gaaagtaaaa
    30481 ctatagtctt agttgaagga tatatggatt taatttcgct ttatcagtat ggtataaaaa
    30541 atgtagtagc aacccttgga acagccttga ctgaacaaca aggcctttta ataaagagat
    30601 atgcggacac tgctattata tcttatgatt ctgatgaagc aggaataaaa gcgactttaa
    30661 gagctataga catacttact aaattaggta tcaatgtaaa agtgttggac ttaaaagatg
    30721 ctaaagaccc agatgaattt gtaagaaaat acggacttag tgattataaa aaagctatgg
    30781 atgtttctac tcactacata aaatataaaa tagatcatct taaaaaagaa tttaatattc
    30841 aaaaagatga agaacgagtg aagttcgcaa aagaagcttc taaaattata aaacaattga
    30901 caagtcctgt tgaaattgat ttttatacaa agtatttaag caatcaaata gatattaatg
    30961 tcgagtctat aaagagagaa gtctatggta aaaattacaa taagccatat aataacaaaa
    31021 atcaaaagaa aatagaagaa aaagtaattg aaaaagtcga agtcagacaa gacggaaaac
    31081 aattagttga agaaactcta ataaaaataa tgttagaaga caaaaaaatt agagaaatag
    31141 cgttacttaa agtagaagaa agtgattttt tattgaaaga aagtaaagaa attttaaatt
    31201 acatgattaa aaatcaagaa ttggacaaaa taactattga caaattgaaa agtttaaaca
    31261 tatccgaaga atacttaaaa gaattaaatt caatttcttt gaatagtata aacttagaga
    31321 atacaaaaga aatagaagga ataataacaa atatcagaaa aaactcatta gaagagcaaa
    31381 ttaatagctt gttgagagaa caacaagaat tagaaaataa taatgatatg aaagaggtag
    31441 atggtagagt tatggaaatt gctttaaaaa tagttgagat aaataaaatt ttaaagagct
    31501 tgtagagaaa ggaggtttga gtgtggaaaa taaatcgaat aaaaaagagt taaaaaaggt
    31561 tactgctaag acattaatag aaaaaggaaa aaagcaaggt tctctgacac ttgcagagat
    31621 aatggaagct ttttcggaga ctgaacttga taaggatcaa gtagaaaatc tttatgagac
    31681 tttgggaaat ttgggaatag aaataacaga aacaaaaaat tataaagctg atatagattt
    31741 ttcggttgct gatgacgatt taagtatagg ccacttagat gaagatgcag aagcaatttc
    31801 acatgaggac tcttctgcaa tagaaataga aactgtggat ttatctttac caaaagggat
    31861 aagtatagat gaccctgtaa gaatgtactt aaaagaaata ggaaaaattc ctctacttaa
    31921 accacatgaa gaagtagaat ttgctagaag aatgcacgaa ggtgatgaga tagcaaaaca
    31981 aaggttagtt gaagcaaact taaggctagt tgtaagtata gcaaaaagat atgtaggaag
    32041 aggtatgctt ttcttagatt taatacaaga gggaaattta ggtcttataa aagcagtcga
    32101 aaaatttgac tacacaaaag gatataagtt tagtacttac gcaacatggt ggataagaca
    32161 agctataaca cgtgctattg cagaccaagc tagaactata agaataccag ttcatatggt
    32221 agagactata aataagctaa taagagtatc aagacaacta cttcaagaac ttggaagaga
    32281 tccaaagcca gaagaaattg caaaagaaat ggaaatgaca gaagacaaag taagagaaat
    32341 aatgaaaata gctcaagacc ctgtgtcttt agaaacacca ataggagaag aagaagatag
    32401 tcatttaggt gattttattc cagatgatga tgctccagcc ccagcagagg cagcagcata
    32461 ttctttatta aaagaacaaa tagaagatgt acttggttca ttaaatgata gagagcaaaa
    32521 agtattaaag cttagatttg gacttgagga tggtagagct agaactcttg aggaagttgg
    32581 aaaagagttt gatgtaacta gagagagaat aagacaaata gaagcaaaag cattaagaaa
    32641 actaagacat ccaagtagat caaaaaaact tagagattat ttagactaaa aagatacatc
    32701 attaatgttt ttgtgataaa tgccatgaga taagtgatat ctcatggcta ttttgtagta
    32761 aagatttaat tttggttttg tagttatttc agtcttatct ataacttagt taaattatat
    32821 ggataaagtg aattgaaagg agaattatat ttgaaattaa cagatagatt attgaaaata
    32881 gcatcattgg tgtcagatgg aaaaaaaata gctgatatag gaacagacca tggatacata
    32941 ccagtatatt tgttaaaaga gggaagagta ccatttgcag tgttggcaga cgttaataaa
    33001 ggacctcttg acaatgccca taaagaagtc atacagaaca atcttttaga taaagttgat
    33061 ttacgattgg gttctggcat agagattctt gaaataggag aagtagaaga agtaataatt
    33121 gctggtatgg gtggaatttt gataagtgag cttctcgaag ctaaaaaaga agtagcacat
    33181 aatgtagaaa aattaatatt acagccaatg caagcgcaag aagaacttag atattacctt
    33241 ttaaacaatg gttatgaaat tttagaagaa gttttagtga gagaagattt tagaatatat
    33301 gaaataattg ttgccaaata tacaggtaaa aatactataa tagaagatga gatatattat
    33361 gaggttggaa taaagctttt agaaaacaag gattctcttt ttaaggattt tatagaaaaa
    33421 aagataaaga catatagttc tatagttagc aaattagaag ggaaaaatgg ggaagctata
    33481 gataagaaga gaaatgaaag tgaagttacg ataaaaaaac ttgaaaattt aataaaataa
    33541 ataggaggaa tatatgctat taaagagtct aactagaaaa attgaaaaaa aataccctct
    33601 aaacttagct gaggattggg ataatgtagg tcttatagtt ggagattttg atatggatgt
    33661 aaaaaaggta ttggtatctt tagaagctaa tgaagatgtt attgatgaag ctatatctaa
    33721 aaatatagat ttaattgtga cacatcatcc ttttatattt gggaaaatta ataaaattaa
    33781 tagtgcagat ttaaaaggta ggcttatcca aaaacttatt aaaaataatg tatcacttta
    33841 ttctatgcat actaattttg atatagcatt tgatggttta aatgactatt ttatggaaat
    33901 tatggaattt ggtaattcaa aggttttaga cgtaactaaa agcgaaaact tatataaatt
    33961 agcagtatac gtgccacata actattcaga tgaacttaga aaggttttaa gtaattcagg
    34021 agcaggtcat ataggaaatt atagtgactg tacgttttca atagaaggtg aaggacaatt
    34081 taagccatta gaaggctcta atccgtttct tgggagtgta aatgatatag aaactgtaaa
    34141 tgaagttaaa attgaaactg tagtaccaca gaagctctta ggtggggtaa taagttctat
    34201 gttagatgca catccttatg aagaagttgc atatgatttg tacaagttag aaaataaagg
    34261 tgagatattt ggactaggta gaataagtaa acttgataaa agtatgacat tagaaagtct
    34321 ttcaaagaaa ataaaagaga aattaaatat gaaacacatc agagtagttg gaaatcttag
    34381 tacaaacata acgaaagtag ctgttgtaac aggagctggt tcagagtttg taaaaaaagc
    34441 taagagacaa ggtgcagaag tactaattac aggtgatgta aagtatcatg aggcacaaga
    34501 tgcacttgat attggtatgt gtatagtgga ttgtggtcat tttgatacag aagatatatt
    34561 taaaaatgtt atgaagagat ttttagatga aattagtgaa attgaagtaa taaaaagtag
    34621 tgtatatttg aatccattta gtactattta gtagtacttg attaaaaaat agtatataaa
    34681 aaataaattt gagtttaatt agggggataa ggatgaaagc agcagtaatt tttcatagtg
    34741 tttgtgggag tacatactta ttagctagag aatataaaga agcgcttaaa gaaatgaata
    34801 ttgaggtaga tatctttaga gtaagtgacg aagttgcaaa gacacttcct caatattatt
    34861 taataaattc taaagaatac aaagatgaat ttgaaagtat aaatgtaatt aaatctggaa
    34921 gagagctatt agattatgat gctatattta tgggttcacc aacatattac ggaaatgtat
    34981 ctggaccaat gaagatgttt atggatagtt tttctgatat ttgggtaggt gctcctttga
    35041 gtggtaaaat atttggttgt tttgctacag caggttcaca acatggagga ggagaactag
    35101 ccttgcaagc tatgaatata tttgctcaac atatggggat gactctttta tcagttccat
    35161 gttcaataag aggaggtcat ccagcgtatg ggatacttca tatagcagga gataactcta
    35221 atataagacc aagtgatgat gttaaaatag gtataagaga ttacctagaa agacttagta
    35281 tataagaata ctatataaaa atacctaaaa gtggctgtat atacttttag gtatttttac
    35341 ttaattataa acgtgaagct ttaccagaaa tatggtcaat ttctatttta acaacagttg
    35401 ttaagttcac atctttctct atgtacttca tgccttcttc aaagaatcct ttagaatatt
    35461 tttttattat ttctacaaga gcatttttct tttcttcatt ttctacagta caagctttcc
    35521 caaaaactat agcactagaa taagtagtac taaatttgtc tggaataaca ttttcattag
    35581 caaccactaa aaaagaaact ttgttgtttt tcaatatatt atctaactta tgaccatttc
    35641 ttgcagagtg aaagtagata gaatcatcaa agtatacata atttacagca actccataag
    35701 gataaccatt ttcagatata gtggaaaaag taccaaattc accatttttt aatacttcga
    35761 tagtatcttc ttttgtcatt tctcttttct tcaatctcat ttctttaaac ataatatcat
    35821 cctttctaat tttgatgctt aaattaaatt ttagagcata tataaatatt atgctatgtt
    35881 ttttataaat tttaataaga aaatatagtt taagataaaa ggttgagaaa gagaatctat
    35941 ttgggggttc atttatgtaa ttattgttag agtattacca acaatattaa taactttaat
    36001 taatgacttg ctgttattta aggtagcaag cttgtctata agaagggaaa gttatcgagg
    36061 atagaagtta tagtttataa tgatattata aagaaaatgt tgttgtagaa aataataaat
    36121 agtagagagg tattaagttg aatataatac ttaatacctc tttaaaatta aattataaaa
    36181 gtgttatgga aactgtacta tacatggtaa ggagataata aagaatgtat ctttaaagtc
    36241 tcaaccaaac agcaaaactt atccaacaaa catacaataa atgcttcttt gtatctagga
    36301 ggcttgacag taagtggcca catgtgcttt aagattatgt ccgtttcttt ttcatttatt
    36361 tgaaaaaata aagttgcatt tttcaatgct tctctagggt gagtaaatcc atgtaatcct
    36421 tttctatcac ctttgtaatg ccaatcataa agaaaaaagt cgtgtaataa agcccctcta
    36481 ataacacttt gaatatcaac atttagatgc aattttttac ataatagata actataatat
    36541 gctactgata aactatgttc aagacatgaa acattgccat gatgaggaaa tgttctcata
    36601 agttgtactt tttctgaaga aagtatttca gaagcacatt cattaaaata tttgatttca
    36661 gcttttgaca acattataca aatctccatt tctagtaata taaatactat atttcaacga
    36721 tatatgaaat atttcttatc ctacaaaata taatacccaa taatacaagt aagatacctt
    36781 ttatggtatt ttaaagtaag agagatttta taaggctaaa caataaaaaa attaaaagta
    36841 aagctatagc aatatatagt atttttccaa gaggattgcc aaaatttata gtccatccaa
    36901 taccaaacct tttattaacc atcaatgatg ggtcatttgg attattatac atgattcccg
    36961 ctatccaata tttttcattc tcttctggag aataagaaga gttcgatttt aaatttggag
    37021 aattaatgta tgttgctatt aaataaatta taactaaaag cgttgtaata ttcatagcag
    37081 ttgatagttt tgttttaatt gataaaatag tagtaaaaaa ctgtattgtc attatagtca
    37141 tcaatattaa aaacaaataa ccaatcttat ttaaatattc aatgttttca attctacttt
    37201 ctttaattct atttgtatct atcttaatcc ttgactttat ggagccaata gaagttatat
    37261 ataataaaac cattaaacaa aagttagtac caattaattt aaatacatta agaaatgatt
    37321 tatctataaa aacatcagga gacccattta tgttccaatg agtggcaatg gagtctggca
    37381 attgattata attaaatact ataaaaatac tcattccaaa agtaaatagt attggtatta
    37441 aatagattat tttaaatttc tttattattt tatttttttt atttataaaa tccatatcta
    37501 ttattaattt tgaatctaca tctatatgac ctaatttaga ataaagttct gatttaagat
    37561 tcttagcttt tttatgtgta tgaatatata gtatactttc atataataaa aaaactatga
    37621 tactaataaa ataggaaagt tccaatttgt taaaagtaaa aaccagaata aaaactaata
    37681 taaggtctat aataaaacct aaagacaaca ggtttttaaa ttttttatct agctttataa
    37741 agtgattata atttttatac tcttggtcta tagaaacccc ataaatgtgt tttttaccta
    37801 caaaataggg cataaaataa aatgtagcat acatcaaaat tagaatcgga aatattgtaa
    37861 ataggcattc aatcttactc ataatatttc acctcataat tgtcaaataa acttgaactt
    37921 aacgataaaa attcctcttt actaatacct gataaataag attttgccac taatagttcc
    37981 aagtcaagct tatttttttg acgagttgtg ttgtctattt tcattggtaa ttgagccaca
    38041 atagcacctt ttcttctatc aatatttaag aatccctctt cttttaattc gttgtaggat
    38101 ttgttaactg tatgtaaatt tacaccaata ttttcagcca tacttcttac agatggaagt
    38161 gattcattta ttttcaattc tcctgaagct atagctttag ttatttcttc tttaatttgt
    38221 acataaatag atttatcgct attaaaatct aaatttaaaa ccataaaaaa cctccattat
    38281 gctttataaa tgaaaacgat tgttatatac atagtataac ataaaaaata ataacaaaca
    38341 caaaatttaa atttatattt tgtaaaaatt gtaatttaaa aaataacata atttttatta
    38401 aaataataca gatactattt atagatagaa ataaaaatta gaaaggagaa aaaatatgtt
    38461 aacatattta atgaaaagac aaatgcataa aaaagatgat ggaatgagta aaccaatgat
    38521 gcttacagga gctgtactta caggtgtagg agcatatgct gtatacaaaa tgataaaaaa
    38581 tcgtaaatct aattctcaaa tggtcaatac aaattattat ggaaatagcg attatgatga
    38641 ttataaatat gacgaatttg attatgactg tgactgtaga gatacaaaac aggattatga
    38701 atatgaagaa ttaaaaaaaa aagtaaaaga atttaattca agaagaataa attgtgaaaa
    38761 ttgtccacat gtttctcaag aagaaatgat gcaatatgta aatagtgtta aagatgtaaa
    38821 ggatgatgtt gatttacatg aagatgctaa aaaagataat atttacaaag aagatgaata
    38881 caaaaaatat gaagaagaat aagtaatatt ctgtataatc aaacaaatag taatattaaa
    38941 ctatttaata tctagtttct aatatgtaag ttgtaactta gcttgaaaga atcggtatca
    39001 ttctttagag agttaagtaa caacttacat tttgttttta taaattcgaa tggctctttg
    39061 tgactatttt gtaattttag atatatttta gtatcaaggg taattgtgta taataaaact
    39121 tgtatatgca gatagtttaa aattaaaatg aaaagaaagg aaatagttat ggtaaagttg
    39181 ttaatagttg aagatgatag tactatctct tttggaataa agtatgcttt agaacaagaa
    39241 ggatttagta tagatatatc aaaagattta tccagtggaa aagaaatgat ttcttctaat
    39301 gaatatagct tgatattgtt ggatgttaca ttaccagatg gaactggtta cgagctttgt
    39361 caatatataa gaggattttc tcagattcct ataatattcc tgactgcttg tgatgaagaa
    39421 gtaaatattg taatgggtct tgatataggc ggagacgatt atataacaaa gccctttaga
    39481 attagagaat taatatctag aataaaagct gttttaagaa gaaaagccaa tactgtagaa
    39541 gaaagtaaga aaatacttaa gtttggagat ttaagtatat atacattgga agctagagta
    39601 tataaaaacg ataaagaagt atttctgaca tcagttgaat ataaacttct tttaattctt
    39661 atacagaata aaaatactgt gttaagtaga actcaaatat tggaaaaact ttgggatgta
    39721 acttatgatt ttgtaaatga caatactcta acagtttata taaaaaggct tagagaaaaa
    39781 ataggtgatg atttatgtga accaatctac atagaaactg taaggggtct tggttataaa
    39841 tggataggaa gtgaaaatag tgtttctata taatccggaa gtgaagaagt ttttaagtaa
    39901 gtatgttact ttgatatttg ttattattat catatctatt ggatttagtg taatcaatgt
    39961 atctttaact aaagatatga ttgtaagaaa taatcaggct ataataggaa ctctatcaag
    40021 taagtatcca aacttagaaa gtgagatagt agatataata actcaaggaa agtctatgga
    40081 aaatacagat tatggtaaga aaatattaag taagtataat tatgataaat ctattaggat
    40141 aaactctgag cctattattt ctaaattagt tttagatact atcaaaataa atataatatt
    40201 agtatgtata atatttattt tgatatttgt gttggttgtt agatatttta agtcaattta
    40261 caatgattta tcagatatga ctaaatatgt ttactatagt tcagagggca agtcatttga
    40321 tatgaaaaat aagaatcaag aagggcagat aggtctttta aaaactgaac tattaaagat
    40381 gacaacaatt cttaatgaaa aggttgaact gttaaaaaca gaaaagatat ttttaaacaa
    40441 tacaatttcc gatatatcac atcaattaaa aactccaatg acttctttaa taatgttgaa
    40501 tgatttatta tataatgata taccatatga agtaaaaatc gattttttga ataagataaa
    40561 aaatcaatta aatagaatgg attggttgat taaaagcatg cttaaattat caaaggtaga
    40621 agcaaaagtg ataaatttta aaaaggataa ggtgaaattt agtgaactca tacatagagc
    40681 aatgcaatct atgaaaatac ctatggagat taaaaatcaa aaactgacta tagagggaag
    40741 cgataatata tcttatattg gtgatattga ttggtctgtg gaagcattag tgaatataat
    40801 caaaaactgt gtagaacata ctcctgagtt tggaaatata actataacct ataaagaaaa
    40861 cccattattt tctgaactaa taataaaaga tgatggtgaa ggtatacata aaaaagatat
    40921 accacatgta tttaaacgat tctatagagg aagaagtagt tcaaaagaag atagtgtagg
    40981 aataggtctt gcaatgtcaa aatctataat agaaagccaa aatggcgata tatatgtaaa
    41041 cagcgaaaaa ggtaagggta cagaatttca tataatattt cataaaatgt atgatagtga
    41101 ctaatctgta atatttaatt cactttatag taatagccaa agcctaatat atgaagtata
    41161 gaatataaat attggaggta gttatggaag ttttaagagt agaaaatcta actaaaagtt
    41221 atggtaaaaa tgaaacaaaa gttgatgcta taaaaaatgt aagtttatca gttgaaaaag
    41281 gaacatttat agcaataaca ggtccaagtg gaagtggtaa aagtacctta ttacatttac
    41341 tgggaggagt tgatagacca actagtggta aggtttatat aaatgatgtt gatatatata
    41401 atttaaaaac taaggacttg gcaatattta gaagaagaaa tataggtctt atatatcaat
    41461 tttacaactt aattccagtc ttaaatgtta aggaaaatat attgcttcca gcagagcttg
    41521 acaatagaga tattgataga gaatattttg atgatttaat gaaaacatta ggtctaaatg
    41581 atagggaaaa gcaccttcca aaccaattat caggaggtca gcaacagaga acctctatag
    41641 ggcgtgcatt gattaataga ccttctataa tattagcaga tgagccaaca ggtaatttag
    41701 atagtaaaaa ttcaaaagaa atactagaat tgttaaaatt atcagtaaag aaatacaatc
    41761 agacactaat aatgataact catgacaaaa atatggcact tcaagcagat agaattataa
    41821 gcatagaaga tggaatcata aaaagtgatg aggtgatgta gatgaatttg tatacatcac
    41881 taactattag atacctaaaa caaaataaaa gaagaaccat tgtaactata atcgggataa
    41941 tactttcgac agctttaatt tgtggtatag gaaacatatt tgaaagtttt atggattatc
    42001 aagtgagaga aactataaag aatgatggaa gtttccatgt tacattttat gatgtaaata
    42061 gaaagaatgt ggagtatgta acgaaatcag ctgaagtaga aaaatatgca tttaccaaac
    42121 agcttggata ttctaaatta gaaaatagtg aaaatggtat agtaagtata aagcaatatg
    42181 ataaaaatgc aataaacgga tataaaattt ctataaaaga aggtagattt ccagctaagg
    42241 aaggagaaat tgttctaagt gaaaatatta ttaatcttat agatgataac ttaaaaatag
    42301 gggataagat aactttaaaa gtaggagata tgtttgattc aagtaagaag aagattgata
    42361 gtatggtatt tcatgagggg gattatatag caagtgaaaa ggatagaact tttgaagttg
    42421 ttggtataat taaaaaacct ggatttgaac gttataatgg aattgcaact gctataactt
    42481 attttaatcc tatggataat tacaatgata caattaatgt atccgtagaa gtaagaaatc
    42541 ctaaagaggt gtatgaagta agtaataaaa taagcaaaaa tatatattca aataaagata
    42601 aagaagcagt ttctgatatg ataaaataca atgaacattt attgaggtta caaggtgcta
    42661 gtaagtatgc agatataaat agctctataa aatctatcat tatagcagta actattttag
    42721 taatcatatg tacaattgct acagtgtata attcctttag tatttctata actgaacgaa
    42781 aaaaacagtt tgggatatta aattccatag gtgctacatc tagtcaaatt aaaagattag
    42841 tttttataga aggtattata attagtttaa ttggtatacc aataggacta atatctggaa
    42901 cagtagccat agacttatta tttaaaatta taaataagta ttttactgaa tcggttgtta
    42961 ctcagatgag tttacggata gtgtataatc caataataat tattgttagt ataataatag
    43021 ttttgtttac aatctttata tcaattttat tacctgcaat atcagcttct aatatttcgc
    43081 cacttaatgt gattaaaaat actggagagt ataaagtgag aaaaatcaaa agctcaagac
    43141 ttattaaaat gatttttaaa actgaaggag tacttgcata taaaaatatt aggagaaata
    43201 gaaaaaaatt tatagtaact cttttttctt taatgataag tgtaataata tttatatctt
    43261 ttagtggatt tacattactt ttactaagaa gtgaagaaat tagaaattct caaagaaaat
    43321 atgatttata tttaactgca aagggaacag ctaggtctat agatgatact gtaagcgaac
    43381 tagaaggtat tgatgggata aaaaactttg aattggcaac aggacaatat gtttctataa
    43441 gagtaagtga aaataaaata aataaatcca aggaagattt aattgggaaa tattatcaaa
    43501 aatataaggt tggagatagt tatgaatatg atttcagtaa taatgattta atttttccag
    43561 gagattttgc aataagaaat aaaataaata acttaataca gggttctttt aataaggaga
    43621 gagcaattca agagaatggt gttatattag ttagaaaaag ctcatttgaa tcaaagggta
    43681 agaaaggtgt agtagaactt accaactaca aagtaggaga tacagtaact tgtgaatatc
    43741 ttgatgaaaa tgcctcaagt aaaaaaataa aggttaagat tcttgctata actgatgaag
    43801 aacgtttagg tatgggttat caaaatatgg gattacaatt tataacttat gatgaagttg
    43861 ctaaaaattt aaacctaaag ttaagtaaaa gtcttatatt tattgattca ggaggaagca
    43921 ttaagactaa gaaggaaata gaagctttgg ctaataaaaa taattttaac ttctatgatg
    43981 aaagtgcaat tactgaaagc gaaaaacaaa atctaaaagt tattaaaata tttgtatatg
    44041 gctttatagc agtaatatct ttagtaagtg ttacaaatat tctaaataca gtaagcacaa
    44101 gtataaatct aagaaaagga gaacttgcag taatacaatc tataggggtt actcctaaag
    44161 gttttagaaa aatgatatat ttagaaagtt ttatatatgg tatactgtca ttgttgtttg
    44221 ggataccgat tagtattggt atgactctaa ttatgaataa acttatttca ggtgtaattg
    44281 agttttctcc gattatacca tggacagcaa tagttatatg tatagtaagt gtttttataa
    44341 taacttttat agcaggttat ataccaataa gtaaattgaa taaagaaaat ataatagata
    44401 atataagaag agaaagtata tagatataaa gataattttt aactatataa aatattatta
    44461 aaatcattta aatgatataa aatattatta tatcacttaa atgatttttt atttatataa
    44521 gaaatctaat aataaattac tagaggtatt taactattta gaaagaattg gtattatata
    44581 aagtaagagg atatataata agaaagaata tttgatttta ttggaaaaca aagaaaaaga
    44641 aggtgattac tatttctcat ataactgcga agaatatgta taaaagttta gaagaaagaa
    44701 taaacaagtt tccacaaggt gctccaccct cagatacatt atacaagata ctaaatgtat
    44761 tatatacaga acaagaggcg aagttagtgg ctcagcttcc aataaagcct tttagagtaa
    44821 aaacagcagc taaaatttgg agtgtaagtg aaagtgaagc atatagagta ttagataaac
    44881 ttgctagcaa ggctctgata ctagatatag aagataataa agggaagaaa tatataatgc
    44941 ctcctccaat ggctggtttt tttgagtttg ccatgatgag aacgagacac gatatagacc
    45001 aaaaactttt agcagaactt tactatcaat atatgaatgt agaagaagat tttatcaaag
    45061 atttatttta ttcaacagaa actaagcttg gaagagtata tgttcaagag gaagttttaa
    45121 ctaatgataa tgaggttagt attttagatt atgaaagagc tactcacata attgacgaat
    45181 caactcatat aggtataagt atgtgttact gtagacatag aatgcaacat gttggaaaag
    45241 cttgtgatgc tcctatggat atatgtatga cgtttgacaa tgtagcaaat tctttgataa
    45301 acaataaatt tgcaagacgt gtggataaag tagaatgtaa agaactactt catcaagcat
    45361 atgagcataa cttagttcaa tgtggagaaa atgttagaaa aggggttacg tttatatgta
    45421 attgctgtgg atgttgctgt gaagcaatgg tagcagctaa aagatttgga aatcttcatc
    45481 cagtacagac aacaagtttt ataccaaata taaaccacga aagttgtgta aaatgtggta
    45541 aatgtatagc tgcatgtcca atagatgcaa taagcaaggt taaggaagat ggtaaagaat
    45601 atataaaaat tgatgaggat agatgtctag gttgtggagt ttgtgttaga aattgccata
    45661 aaaatagcat tatgttatta aagagagatg aaaaaataat aactccagca aattcagtac
    45721 atagagcagt tctcatggca attgaaaagg gtcagttaca aaatttaatt tttgataata
    45781 atgctttagc tagtcataga gctatgggag ctatactatc agctatatta aagcttgaac
    45841 cagctaaaaa gattttagct agtaaacagt taaagtcagt atatttagat aaattgttaa
    45901 gtatgaatga taaatagaaa ctaacttaaa aaagacaagg tttttaggct tatctacatc
    45961 ttaataaact tattatttaa tatctaaatt taatttttac acatataaaa cttataattg
    46021 aaataaaaaa agactatatt caattaatat tacttgaata tagccttttt tagttatgtt
    46081 aagctatttc ttttatttgt ttaaatgagt tttcatttcc atttccacca atttgtgtta
    46141 tagatttaaa tctcatagtt ttaaatagtt ctttttggtt ataatctagt gagtttccta
    46201 ctagtattac aggagattta gtttttccag ccaaaactcc aacagataat ccatctatta
    46261 aatcatcttg tttattcatt ccatttttag caacatataa gttatctatc ttagaatcct
    46321 tgtaaaattc ttttattact tttgcattag tttcatttct agtgctacct gatattctct
    46381 gtggattttg aagtttgctt tctatatttt tagaaactgt atattctcca ccgattacat
    46441 aagatttatc aatttttttg tttttaaata agttatttat ttcagttata ttactatttt
    46501 cattagtaag tattataggc atgtcatttt gggctgatac agctccaatg cttactgcat
    46561 cagctaatcc tttttcacca ttaactactg aaatttttga tatattacta atattattca
    46621 attctttagc aatgttcata gaagtttgat atctatcact tcctgaaatt ctagagacag
    46681 agatattatt tgattttata gtatctagag aattttcatt tatagaagcc tctccaccaa
    46741 ctacatatac atttttaact tttaatctct ttaattcagc caaagttttt tcatttattt
    46801 gagagcttcc agttaataat ataggagcat ttttcttgta tgcaaatggt gttgcagcca
    46861 aagcatctgc aattgctgag tcgtttatta aaactgcatt ttcagcattt tcccaacctg
    46921 cttgacttat cttaatagaa gtttcatatc tatcagttcc tgtaagtgca cttgcattta
    46981 cttcgtcttg atatgtaact tcaaatctag catttggact tcctaagaaa acaattacgc
    47041 ctccttttgg caatctaact ctattgttct ttgtaactaa agaatagttt acaggaactc
    47101 ctttatcatc ataaacggca aaagatgaat tttgaggaag attaacttct atttttttat
    47161 ttgctatatc atcaccaatc ttataccatt ttgcataacc attagatgct agttcgcaag
    47221 taaaagattt ttctgctggc aaattagtta tagaatcttc actaacatat gtttgtgtgg
    47281 caaaagataa atactctgta ccattttctt tataaagttt aatatcggat aaatcacgac
    47341 ctattacacc aggtatttct ataaaagcat ttgaattatt ctcatctatt atcttagtat
    47401 taactatata acctggagtt tcatcagaaa ggccaggagt tgccttcaca gaaccaaaca
    47461 tatagctttg agaagtatat ttttcatcta ctagatagta acctttacca tttctttttt
    47521 tccatacttc ttttacacta tttgatatat tgttatcatc taccttttga gctacatagt
    47581 ataaacttgc tgtttgacct aatccaggga catcatcata agagctcata tttagatatg
    47641 taatattatt tttttctttt acaaatttta aacaactatt acctttttca gatacaaatc
    47701 tatcttgacc tatgtatatg tatttatctt caggtccatt ttctatataa ggagaagata
    47761 ccgttaaagt accattatca ttaacatcaa cttttaccat atttgaagaa gcataaagcc
    47821 cactattttc ttttaaatga cttggcattt taacttgttg tggcttacta aatgttttat
    47881 caggttttat ttctttaatt tttccctttt cttttaaggc agacaataag atttcttgtc
    47941 caattatttc atcaaattgg ctatttccac cagaagaaag aactgcaact gccatatttt
    48001 catctggaag aactataaga ttactgtgga atagtaaaga atctccacct tttgtaagag
    48061 cttttaaatt gtactgatta aatggataag tattgacaca atcccatcct aaaccatatc
    48121 caagtataga atcttctcct tcaggccata gaccatttaa atattctttg ttttccatag
    48181 cttttactga agcaggagaa agaataccat ttgagttttt cataaatgtt tgtgcaaatg
    48241 tacatagatt ttctgcactt gaatataaac cacctgcccc tatagcatta aggttatctt
    48301 gtggaactgc atcctcccaa taaggtacat atgcttttgc tagttttgaa ctatcaaaac
    48361 tattttctgt agtttttgta ttttgtaagt taagtggatt atttatgtat ttgtcaagaa
    48421 aattagtaaa tgacatacca gatactcttt ctactaaaat ttcagctaaa gtaaatccat
    48481 cattacaata aacagaaaaa gctcctggtt ttgcttttaa tctttgtttt tgtaattctt
    48541 ttaagaagtt atcatgacca taagaatcat tgtctcctaa taagagcgta tttttaaagc
    48601 tgcttcccat caatcctgat gaatgattta ataacattct tggtgtgatt tccttgtatc
    48661 tattatcagc cattttaaat tcaggtatat aatttacaac aggagtatct aaatttactt
    48721 ttccatcatc aaccaacttc atgacagctg ttgtagtaaa cattttactg atagatgcga
    48781 tactatacat attatcctta gttaaatttt tgttatcttg tttgctgtaa actccaccat
    48841 taccagaaat ctcaatttta ccattatcaa tcaaggcata ttgagcattt acaacaccat
    48901 actttgatag caaagtttgg attttctctt tagcaagagc tttagttttc gtgtaagatt
    48961 ccgagttttt actataatca tgtaattcta tatcagaact ttgatatttt tcggaattat
    49021 cttttgtatt atcagcaaaa gtaatatttg gagcagcagt aaataacata ctcaatacta
    49081 atgtactaga taataattta gagagctttc gcttaaacat attacattac ctctttccct
    49141 ttttaataat ttataattag cataacattc ttaattattt taatcaacag attattatca
    49201 ataaaaa
//
LOCUS       DAFNNB010000010        47640 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0010, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000010 DAFNNB010000000
VERSION     DAFNNB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0010"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     regulatory      106..191
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            352..873
                     /locus_tag="L3M60_000500"
     CDS             352..873
                     /locus_tag="L3M60_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBN6225203.1"
                     /translation="MKLKKNKKGFTLVELLVVIAIIGILAVVAVPALFSNINKAKVAS
                     VESDYSSIKSAALSYYSDTNKIPVTPDGQTGLNVLETYMESLPDKADIGGEYKLIKVG
                     NKLVLQIGKDGEGVTLTEAQSAKLLSDIGKDKIYTGVTGDNFGEQLKDTTKIDNKALY
                     IVLIDNTVMDSTK"
     gene            1024..2700
                     /locus_tag="L3M60_000501"
     CDS             1024..2700
                     /locus_tag="L3M60_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HBN6225204.1"
                     /translation="MAKKVRIGDKLVEKGYITEDQLKWALSEQKNSGKRLGEFLVQEG
                     LIDSNLLISVLKELLDIESIFLEGTEIDTLATKMVPENICKRYTVFPFKIDGNKICLA
                     MSDPQDREAVQDVRRMSGKDVEIFISSTEDINKAIGHAYAHSEINKAMTEYNKNRTGG
                     VRETVILEEDVNAAPIVRLVNNILENAVRMEASDIHIEQSENYMRVRFRIDGMLREYM
                     RMNSAPYKAVISRIKIMSDINISEKRIPQDGRIYLKVDNKPIDFRVSTMPTNRDEKIA
                     MRVLDKSNFMVSKEVLGIDEHGSKIYDELINTPYGLILVVGPTGSGKTTTLYSMLNQL
                     NTENRNLLTIEDPIEYELPGVNQSQINEKAGLTFASGLRAFMRQDPDIIMVGEIRDTE
                     TAEIAIRASLTGHLVLSTLHANTAVGAISRLLDMDVESFLITSSVLGVISQRLTRKIC
                     EHCKVSYEADIGEKKALGIDVNESVTIYRGKGCERCNNTGYKGRLGIFEMLEITPEIK
                     ELIDSSANQREILKMARKQGMVSLKEDIVKKVLNGKTTVEEMIRIILMTD"
     gene            2712..3920
                     /locus_tag="L3M60_000502"
     CDS             2712..3920
                     /locus_tag="L3M60_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBN6225205.1"
                     /translation="MKSFKYTVIKEDGKKQTDVIEANDFNEARNLLRKRNLRVIEIKE
                     SKNKNIRLSSRKRKKDLGADQISHFCRQFAIIVSSGINSISGLETLARRSTNITLREE
                     INRIVAEIKVGSTIADSMLSSKSKFPKLLGAMVATGEATGKLDEVLKSMASFYASEHR
                     VKQKLRNAATYPLIVLISSFIMIFIFTAFMVPKMINSITTVGATLPLITRIVMGFGMF
                     MKKFWIIVLLVIILFIYQFKKYLKTPIGRAHKDRVINKIPVLGKGINCMVATRFSRAL
                     YLFVSTGYPLVQGLDYIIDSVNNTMAEKILVSAKDGITRGEGLAENLERYTYFDSVLV
                     QMIAIGEQTGELENISKQMAEFYEYESEIYLNRMASMIEPVLIIAVGIIVAILVVSIF
                     MPMLSIYDAM"
     gene            3935..5647
                     /locus_tag="L3M60_000503"
     CDS             3935..5647
                     /locus_tag="L3M60_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HBN6225206.1"
                     /translation="MNKVYVSYYGDSISIVEGRYKHNKDKFYIKNLNVFSIEDIAQDF
                     SKEKYALLRYALENVKLKSRHVVFCLNTRDVILKPQKLPNMDKKDLDGFMKLEMDEMM
                     ALESDEYVFSYEVSSETPDNSDGDESSLNLIIGAIQKEEVNAIVEIIHEFDLILDRID
                     TLSTAYLRMLKNLDYNDIMVTNISDNSTIINIYKKDTLFISDNIPIKITAKDMDMQLL
                     NVVEESKGLMNYYSSRNFGKVTDTILILGKRYSNKLIYETFSSAFSSYVSQGLTEIMD
                     ISDMITGDIDEDDINSIVELLGSMIEPKGKRDFENINFLPMDILRKQLKAKQMKNILK
                     IAPVVILILAIPYLCLIFSNNLANKELENVNAEIRNIESAYYQIGNIEKMIQSKSEEI
                     KVYDMLIGKKVKWENLLDDIDKNTPSKVELTSLSTTYQNLGANNKADNSSSNKTTTDK
                     SSNNETKSNTKSDSNGDKTNNDLNEDKTTNVSEALTGNSETNSNNTDKDDKTPLYDKI
                     PNVINIEGNAKDSSYVGQFLYKLKGLYYFKDVKLHSIKSNAEDSTYTFSMTLYLKEGV
                     LTNE"
     gene            5640..6548
                     /gene="pilO"
                     /locus_tag="L3M60_000504"
     CDS             5640..6548
                     /gene="pilO"
                     /locus_tag="L3M60_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HBN6225207.1"
                     /translation="MNKDIFKKDIKDIFKMEVSLKGVLIKVIILLSILSYGYFGLYPQ
                     YQKYIVTKNSVKQATEKLNIYKEKTALMPKYEKQLEEVESELKEKSKNLLYDMQDGLF
                     LIGLDKKMKAYNIDLTSYDVGNVREYEHFYGVSTTISVRGDYRNVKKLMAYLEEQKNV
                     TQILDYTMTSYIEPEKEEEETTNNKDLIKNVNINKDTKIYWVEGENKWHLYKDCPLIK
                     EGEILKNDKLLDRSTSYLCNTCVLRAATEKEAKKEVVAPKAEGVVDATFKFIMYTKEN
                     TTMILDTDNPSSWRPGKYNPFKSSAK"
     gene            6575..7141
                     /locus_tag="L3M60_000505"
     CDS             6575..7141
                     /locus_tag="L3M60_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBN6225208.1"
                     /translation="MSLYKNNEKGLTLLEVIIAVFILTIVLSISYKVFNGITSAVKKQ
                     QIITDAQVNINLINKYLNRDLENCKELTKTGSGSNYEYNIETPDNIVKYEVSIETKKN
                     TEVYSVTRIEKNTIDTENEVREEIIYNQPLVQNNKEMKETPFKIEKQTDKSIYTVSIF
                     YNESVQEGHKNSNLNNKTYTFDVMSRIG"
     gene            7177..7704
                     /locus_tag="L3M60_000506"
     CDS             7177..7704
                     /locus_tag="L3M60_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HBN6225209.1"
                     /translation="MSKKNSKRGFSLIEVLVAMAIMGIILFAFFNIINTNNKANIKND
                     TDINSLNYVQSEIENLREKIKSGEFDFDSLDKMEDGTVVYEKLIDKSKKIVYDKVLSE
                     GNVSLYDTPYEKITTIKDEDGNLIDKENITNKIKTIVEDKSGQIYKIAVTGKSMNDYS
                     SKKEVKIVTEIFKDK"
     gene            7725..9263
                     /locus_tag="L3M60_000507"
     CDS             7725..9263
                     /locus_tag="L3M60_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225210.1"
                     /translation="MRKWNKFKSERGAALVLVLVVVALLSIVGLMFSNQIANRIKSTK
                     TTNEGIQAKYLAETCVENSIDKAYEKLYDELEKMDNEFKSENQQKSISRSKLRNISDE
                     DFNNQNEENLEVERLGYMNNINFYLNKASSDLEKASIELKKLYDLDMLDYRDIEYVDE
                     NIISHRNSILDICKNYTSGDISKINEYILDEDINSTTLVEAKLVNNDILLKMFLEENK
                     IENEHLNSAFSYTYKALDKISLAMQNMIEYRHTFHIDEQKVEVSNGIPNSQQYYELIQ
                     NPIINSKEYSWNSKWDTLENLLETLPNQTQGFNSLRVHLRNNVRKFEELSDNISSGKK
                     NTAKNFLKYKELLYEISDQCNQLKSMSYEKIPVKYDNMALITTFDYIQNELLAEIKCR
                     LKELKPQEVDKTEDITIKIPFYKADYDMTKEGWPKLKENGSGAELSLMVTGDKDGIKE
                     VEVTDSKKNIIGLGVEENSNSKYKVEAIINFNLNIDTNIVGNYDIKDKILINHDISSY
                     KKVN"
     gene            9363..10445
                     /locus_tag="L3M60_000508"
     CDS             9363..10445
                     /locus_tag="L3M60_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBN6225211.1"
                     /translation="MYIYDLLEQGIRLNASDIHITVGTNPVARVKGGFVKLSEQILTP
                     EVTMQMAKDIAGESMFKVIKEHGEADFSASLKTGERFRVNAYRQKGNYAIAIRTITAE
                     IPTFEKLGLPENIKSFTEKHKGLVLVTGPTGSGKSTTLASMINIINEKQQKHIITLED
                     PIEYVHHHKQSLVNQREVGTDTESFHSALRAILRQDPDVILIGEMRDPETVSIALTAA
                     ETGHLVFSTLHTVGAAKTIDRIVDMFQPSQQQQIKTQLSTVCEGVVSQQLLPTADGKG
                     RIAAIELMFATPAIKNLIREGKTYQIPNMIQTGVKSGMKTMDQDLMELYKNGKITKDM
                     ALSRCTDQEFMTRMIGGVNYNGYYNR"
     gene            10426..11208
                     /locus_tag="L3M60_000509"
     CDS             10426..11208
                     /locus_tag="L3M60_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBN6225212.1"
                     /translation="MDIIIVNLYVFIVGIVFGSFFNVCIFRIPEGISISRPPSHCMSC
                     NTRLKPIDLIPVLSYLISGRKCRYCYEKISSRYAIVELITGILFLIVFMLYGISTSTV
                     YYLVLVSLLIIITFIDIDHFIIPDEILIFGAIFAIIFNLLFKEIPVKDSIIGFFICGG
                     SVWLIVLLIEFVIKKECMGGGDIKLFAMLGLYMGVKNSLLTALLSVYVGAVYGICVII
                     ISKTKGKEYNSIIPYGPFISVGALITILCGKQLLEFYFSMIT"
     gene            11310..11960
                     /locus_tag="L3M60_000510"
     CDS             11310..11960
                     /locus_tag="L3M60_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBN6225213.1"
                     /translation="MEYIIIISLFFAHLINKSLCVFCDREKKNLYNTFFIYIITVVLS
                     VLIYLKFGISVECVQYMVLIPFLIIISIVDYYTIYVYDITIVSGIIVQGIIFLVFCNI
                     VQFDYINHIFGLVIGFIIPYIIAKLTKGLGYGDVGLYSLCCFALGNNYGFYVIVLSFI
                     LAFLFFIILYITKIFKDIDIKKQIPFTPFISLATILIMFTEYNIFNFYYSLLYKFI"
     gene            11974..12534
                     /locus_tag="L3M60_000511"
     CDS             11974..12534
                     /locus_tag="L3M60_000511"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBN6225214.1"
                     /translation="MYVVVGLGNPGKKYEKTRHNVGFDVIDILAKEYNISVTKIKHKA
                     LIGEGRVGTEKVLLVKPQTYMNLSGETLIDIYKYYKVDLSNIVVVYDDIDLEVGKIRI
                     RKKGSGGTHNGMKSITKCLGSNDFPRVRVGVSKPEVGQDLADFVLSRFRKEESDNINE
                     ALEKAAYAIDSIIRENIDMSMNKYNG"
     gene            12551..15937
                     /gene="mfd"
                     /locus_tag="L3M60_000512"
     CDS             12551..15937
                     /gene="mfd"
                     /locus_tag="L3M60_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBN6225215.1"
                     /translation="MNDVFLYPLQNSKEYKDIINCIKNTKGSLLVNGLLPVQKPHISY
                     SIFNDLSRQMIFITSSDLEAKKVYEDLSFYIEDKVEYLGFQDIYFYHLDAKDRNEEAK
                     KLKVLLKLANKEKIILVTSIEAVLRKYIPKQVLLDSVSHYKVGDNLDLERLTEKLVSL
                     GYERVSKIEGFGQFSIRGGIIDVFSLEYTNPIRMELFDDEIDSIRTFDVYSQKSIDKL
                     QQFSITPSREFIYPEKTTDALVKLKKETTKNTDEGTFQNIDYISSKTYFEGVENYIDY
                     IYPEENKSIFTYLADDAIVFINDITRLKERCENYINEFRENYKLNLERGLAIKNQGKL
                     LYHYTDLEYLVKDKSVVLNSLLPKSINNFSIKSIINFESREVPTFNGKVDLLVEELNR
                     LKYNGYKIILATNTLERANKLGKDLLDKGLETTISKDRDIEIKSSQVIIVPAHINSGF
                     QYKSIKFVVITDNEMIGVYKRASKTTNKKVKKGKKIESFLDLSVGDYVVHENSGVGRY
                     TGIEQITVNAIKKDYMKIVYQGGDNLYVPIDQMDKVQKYIGAEVEKVKLNKLGTNEWT
                     KAKAKVKKEIEDMTKDLIELYAKREKIQGYKFSKDTPWQAEFESLFPYQETEDQLKAI
                     EETKKDMESSKVMDRLVCGDVGYGKTEVAIRSIFKACMDQKQVAVLVPTTILAQQHYN
                     TFKERFENYPLRVEVLSRFKTPKQQKQIIEDAKKGLVDILIGTHRIISKDINLPNLGL
                     VVIDEEQRFGVKHKEALKKIKSTVDVLTLSATPIPRTLHMSLSGIRDMSVIEEPPQER
                     HPVITYVTESKESVIQDEIERELSRGGQVFFVYNRVEHIEEMASMIQKLVPDARVAVA
                     HGRMTSKSLENIILGFLNKDYDVLVCTTIIETGMDISNANTMIIYDADKMGLAQLYQL
                     RGRVGRSSRQGYAYLLYEKDKTLSEIAEKRLKAIREFTEFGSGFKIAMRDLEIRGAGN
                     ILGSQQHGHMAVIGYDLYVKMLNDAIKKVKGEPIVEEIDVEIDLSVNAYIPDNYIKDE
                     LIKIEMYKKIASIENKEDMLDIQEELEDRFSDIPKPVQTLLTIAYIKSLCKILKIEKI
                     RQLKDEILLVPITKYRTKQKIGYNIVTELEELLEKMCKVK"
     gene            15968..16963
                     /locus_tag="L3M60_000513"
     CDS             15968..16963
                     /locus_tag="L3M60_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN6225216.1"
                     /translation="MKKVITLVIAMILVVSVTACNSSKGETVATVEGTKISSDEFKKT
                     IALYKDSMEQTYGKDIWDKEVEKGVKYKDKFKDLILDQLITTEVIYSQAKKDNLLPKK
                     EDVEKSFKELKDAMGKDEKYKEQLKKLGIDDEFLRDQQEKDLAMQNYQSNFTKKTKIS
                     DEEMKKYYDTHKDEFKKDEVEASHILLKTVDDNNKPLSAKEKAEAKKKAEEALKEVKS
                     GEDFAKVAKKYSQDASASDGGKLGFFSRGQMVAEFEDAAFSMKKGEVSDLVETQYGYH
                     IIKVTDRINEQTSFEDAKETIKDQLLKNKYQEQIEKLTKEAKVEKDEKVINKITI"
     gene            17086..17625
                     /gene="spoVT"
                     /locus_tag="L3M60_000514"
     CDS             17086..17625
                     /gene="spoVT"
                     /locus_tag="L3M60_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HBN6225217.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRKTLRIREGDPLEIFTAKDGEV
                     ILKKYSPIGELNEFSQEYAETLAEVLGYGVLITDLDSIIAVSKLPKKDYKEKSISDEL
                     EAIISERTVKYSKDKNILPLFKEDQKEYTSQVIMPIISASGDCIGSIVVVSKDKDTIA
                     ESEEKSLKIAANFLGKQVQ"
     gene            17745..19358
                     /locus_tag="L3M60_000515"
     CDS             17745..19358
                     /locus_tag="L3M60_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBN6225218.1"
                     /translation="MNNNKNKDSFLKGALILGLAGVIVKIMGGFFRIPLGNMIGSEGM
                     GYYQAAYPVYTLFLTLATAGFPTAVAKLVSEKVAIGNFKGANKIFKVSHTVLFITGII
                     SFCILFFGADYIVTNIMKNPGALYSMKAIAPALLFVPAMSAYRGYFQGRQDMTKIAVS
                     QVAEQFFRVVLGLTLAYLLMKSLGQTFGAAGAISGATIGSIASMLYLMFAYMLGRKER
                     RAEIDASQRFKDERVSYIFKKLLTVAIPITIGASVMPLVNMIDNVIVIRRLMEAGFTY
                     KVANSMFGQLTGMAMATINLPAVITTAMSMSLVPAISKAYALGNKSKARKDTKSAVKV
                     TLLIVLPCAFGMASLAIPIMGLLFPHEPSSVGTILFTLTPCVLFLGLIQTLTGIIQGM
                     GKPIVPVIALCVGMLCKIVISYTLTGIPDINVLGSAFGTVTAYFVAAMINLLYVKKHM
                     NVNFSKKEFIIKPFITVMTMFIMVKLSYGALVGFLGNSISTIIAICIGGIVYVVVILG
                     IGGIKKEEILTMPKGDKLYKLLKKVKLIR"
     gene            19465..20913
                     /gene="mazG"
                     /locus_tag="L3M60_000516"
     CDS             19465..20913
                     /gene="mazG"
                     /locus_tag="L3M60_000516"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBN6225219.1"
                     /translation="MGKISIVGLGPGDYSLISQGVLESLKNSPKVFFRTEKHPIIDKL
                     KETIQYTSLDYFYEKEENFDNVYLKISEFIIEESAKGDLVYAVPGHPRVAEKTVSIIE
                     RLANEKHIAVEIVPSMSFVDAMFNYLAIDPSEGFKLVDAFEIDNSYIDLDTSMIITQV
                     YDKFIASNVKLKLMEYYDYNQEVCIVNGAGVKDLENKKFIKLCELDRNENFFDYLTSL
                     YIPKSSKKMYNTVHDLEIIVNTLRSPSGCEWDKKQTHQSLKNSVIEEAYELCNAIDNN
                     DIDEMVEELGDVLLQVIFHCQIGNEEGYFDLKEVVNGICKKLIHRHPHIFSGAKLDMS
                     KFEKTWEELKKEEKGESTITDGLRRIPKHLPALMKASKVQHKAALVGFDWDNVEDVFK
                     KIEEEYQELLDEHRVGNIKYIKEELGDLLFSIVNLARFLDIDSGEALNCTTEKFINRF
                     EFIEKSAMNLNKKLEDMTLEEMDEFWNQAKKQ"
     gene            21002..21280
                     /locus_tag="L3M60_000517"
     CDS             21002..21280
                     /locus_tag="L3M60_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003153447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HBN6225220.1"
                     /translation="MNKAELVSKMAEKSGLTKKEAEAALNAFMSSVQDALVNNEKVQL
                     VGFGTFETRERAARQGRNPRDPEQVIDIPASKAPVFKAGKGLKDIING"
     gene            21352..21594
                     /locus_tag="L3M60_000518"
     CDS             21352..21594
                     /locus_tag="L3M60_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HBN6225221.1"
                     /translation="MRLDKFLKVSRIIKRRTVAKDACDKGIVTINGKVAKSSSEVNIG
                     DTLEIQFGEKKMKFKINEVKEHVLKNDAKEMYEILE"
     gene            21656..21910
                     /locus_tag="L3M60_000519"
     CDS             21656..21910
                     /locus_tag="L3M60_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HBN6225222.1"
                     /translation="MEQNITLKDRSKLVISGVEHIYSFNDKRVELKTSVGEMVIEGEN
                     LDMSKLSIDENIISIDGTINSMVYAKPKKPQESFLKKVFR"
     gene            21911..22444
                     /locus_tag="L3M60_000520"
     CDS             21911..22444
                     /locus_tag="L3M60_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225223.1"
                     /translation="MIPFTQDVSIFYATIYGGILIGVLFDFYRGLRGNFKFINYFAII
                     FDVLFWFLATVIIFVTINLTEFFDLRYYHFVALFIGFILYYNTISKIVLSIINKIIRF
                     VRNSFKKVTHYIVSFLNNLYYVIIYSLHLLFDIIFYIPNIFIATRKSIKRKSNKKSKN
                     KKKSKPKKKKNKTKKRV"
     gene            22450..22734
                     /locus_tag="L3M60_000521"
     CDS             22450..22734
                     /locus_tag="L3M60_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HBN6225224.1"
                     /translation="MNLRKKFSGQIIVISLFLGISIFSMMTGFVFEYTKAKEYKKEIA
                     SLNKQLKKTEIQINALKKDEKSYEGDLEDIARKRLNMVKPNETVYVDINR"
     gene            22847..23767
                     /locus_tag="L3M60_000522"
     CDS             22847..23767
                     /locus_tag="L3M60_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HBN6225225.1"
                     /translation="MKVGAIDIGTNSMRLLIADYRDGNFINRKKFVNTTRIGYGVDSK
                     GYIKEDAIKRNIKALKEFSNKCNDEKCEKIYCMGTSALRDSKNGDYFVKLAKEETGID
                     VDIVTGIEESNLGFKGVLEGIQDDDTIIVIDIGGGSTEFIVGDSEGIKFNKSENVGAL
                     RMTERFLKHDIIEDEELEAMSSFIYDEIDKTLNHIKYRGLKTLIGIGGTITSLSAMNQ
                     ELEVYSMEKIHNSKIHKKEIEEILQKLIKMTLSDKKNLKGLQEKRADIITAGVKILNI
                     IMEKLEIEEITVSEYDNLEGLICQKSKKMY"
     gene            23857..26229
                     /gene="spoIIE"
                     /locus_tag="L3M60_000523"
     CDS             23857..26229
                     /gene="spoIIE"
                     /locus_tag="L3M60_000523"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein E"
                     /protein_id="HBN6225226.1"
                     /translation="MQRSVAVVKNKTVKPNVIKLISGYIDFTTAAFAVMGFLLSRSII
                     VDSVAPLGIAFFICASKIDKYKIPVFISTLLGILLSSNDFGNVAKYAVCLTIFMAMNK
                     KLKLSDSIAKVAFIGAIILLPISIGQAFFSNRYVYGLLIAGVEAIIMFISIYVFSYGI
                     GLMANVNNRMSVRTEEAISISLLLTFSIMGIGDISLFGISIRTILSTVLILLTSIVGG
                     ATMGASSGVIIGVASILNNMTSAIYMGIYSFSGLISGAFNKINKYFCILGYILSWTII
                     YLYTSGITSNMMQLRDILLGCLIVLVLPERLFNKIEKLIKSNVASNEIVYDYIMRSKN
                     LTNSRLNSIYKTYDDLADTFDKIREKDKVLDQRDIANVIDMIHNDECKSCSMRRMCWE
                     SRFNHTYTMIYNILEKIEEKGELSLNDIPKNFRKECMKPESIVKISNHYYKMFVLDYD
                     WSVKFSESRKLIANQIRSISKSIKSLSQDLEGDIMLDIEKEKNIYEQLERYDITVDKV
                     SYLTKSNSEFEISIEKKTCHDGCMCEDKIVNIISDLVGENMSVRKIGCHCLGGKCKAT
                     FVKSQKYKAVTEVSAMSRDGHILCGDNYTYMEINDGKYMMAISDGMGKGKKAYEESSA
                     TIDILEKMIDAKIKDEIVIDTINNMLLLKSSEEMFSTLDLGILDLKRGCLETIKMGAC
                     STYIKREDGEVDLISSSSLPVGILSDVKIDRKNVKVKEGDYVIMVSDGIVDAGRNNNL
                     GDNWLIYFLKNIETTNPKEISNLILDRALELQALQIEDDMTVLVTKICTK"
     gene            26580..28472
                     /gene="pepF"
                     /locus_tag="L3M60_000524"
     CDS             26580..28472
                     /gene="pepF"
                     /locus_tag="L3M60_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HBN6225227.1"
                     /translation="MNRKLCKAIIPLFISIFCMLIISIKADQGYQKNGDRAITVMNNN
                     DTEDDAEKKQEIDYEKTCWNLQSLFKSDEQWKKELKSFDKDTKELKNYIGKVTKSPTH
                     LSFALNIKEKLDIRLNKLSAYVKLKQDTNKNSYKYLDMSDSMSKSYGNYMGICSDLEL
                     EILKLSNKDYKKIISNKNINRKYGAYLRDIRRNKVHYLDDKSEDILSKVSSISSLPSQ
                     VYELFRNMDRKTNLTPAQYASELESSDRENRKKAYQDELITYNDNINTIAGLITGQVK
                     KNIFYSQERGYESSLEMYLESDNVDEKVYNNLIRTVNGNMDSLHKYIDLRKKVLKLDK
                     VHSYDMFVPMVKAVDNNINYEKAQSIVYSALSPLGKEYNDVVYKAFNEKWIDVYSHEN
                     KVSGGYCLSVYENHPYILLNYNNSLGSVSTLSHELGHAVYEYLSSKNQNYFNSNPSIF
                     THEVASTTNEALLYEMLIKEAKGNDEKAYYITQYLDLIKDTLYTQTMYAEFEKTIHEL
                     VEKGKSVNALVLNDVWGQLLKKYYGQSYELDQLSKVGWARIPHFYNSFYVYKYATGCS
                     AGVSFAQDILKNGSDNYMSFLKKGSSDYPINLLKSSGINLTNTKPIENTIKKFDSLVA
                     ELEKLL"
     gene            28586..29551
                     /gene="galU"
                     /locus_tag="L3M60_000525"
     CDS             28586..29551
                     /gene="galU"
                     /locus_tag="L3M60_000525"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBN6225228.1"
                     /translation="MQVKVKKAVIPAAGLGTRFLPATKAQPKEMLPIVDKPTLQYIIE
                     EAVASGIEEILIITGRNKKSIEDHFDKSVELELDLEKKGKKELLEIVQNISNMINIHY
                     IRQKEPKGLGDAIYCARHFIGDEPFAVMLGDDIVDNDVPCLKQLTDAYEEYRTTILGV
                     QKVNQEDTNKYGIIEAKNIEGRVYKVKDMVEKPESGKAPSNIAILGRYIITPEIFDIL
                     KDLPPGKGGEVQLTDALKILSKKEAMYAYNFEGKRYDVGDKLGFLEATVDFALKKEDL
                     KEDFIKYLKHVCSEFDKNNNVLYNNTDEKIDLIEVNEIRELEIQK"
     gene            30051..31655
                     /gene="rho"
                     /locus_tag="L3M60_000526"
     CDS             30051..31655
                     /gene="rho"
                     /locus_tag="L3M60_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBN6225229.1"
                     /translation="MRRIFLILDDKVLEGLGSKTLVELREIAKELKIKSITTYKKNEL
                     IEIINSKSKNEEKINEETKEKNEDIKEDIKNRKENIERESIEKEVEYKSPTKSYNKKE
                     IDTQNISQNQENRNQRNDYNKTTSVNDCNKPSNISNRMVRNNNKNYYMPKQVDESKIV
                     DEFNTSKEDEVVGVLEILPDGFGFLRGSNYLSTEGDVYVSPSQIRRFNMKTGDKIKGI
                     TRHPKSGEKFRALLYVQKINDENPDTAIQRNAFETLTPIFPEERLTLETNRNEIATRI
                     IDLISPIGKGQRGLIVAPPKAGKTVLLKSVANSIAKNHPNVELIVLLIDERPEEVTDM
                     KESIEGDVIYSTFDQVSSHHVKVAEMVLNRAQRLVEHGKDVVILLDSITRLARAYNLT
                     ISPTGRTLSGGIDPGALHGPKKFFGAARNIRQGGSLTILGTALVETGSRMDDVIFEEF
                     KGTGNMELHLDRKLAEKRIFPAIDIYKSGTRRDDLLLDDEEKTALWRLRREMSNNSVM
                     EITDKVIELIKRTKDNKEFVKSIKNL"
     gene            31808..32008
                     /gene="rpmE"
                     /locus_tag="L3M60_000527"
     CDS             31808..32008
                     /gene="rpmE"
                     /locus_tag="L3M60_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018591942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBN6225230.1"
                     /translation="MQKEIQPKYNPVEVRCACGNTFVAGSTKDEIKVEICSECHPFYT
                     GKQKNIEKGGRIDKFKKRFKMD"
     gene            32153..33046
                     /locus_tag="L3M60_000528"
     CDS             32153..33046
                     /locus_tag="L3M60_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HBN6225231.1"
                     /translation="MAKQAVGGQAVIEGVMMQSKDKRAVAVRKSDGEIALKEDRIKSW
                     VRDKNIDKIPFVRGSFVMIDTMIQGIKSLNFSSEFFMEEAEEDKFDLFIKKIFKDKAN
                     DIIIIFSLVIAMLLSAGLFIFIPTLVGGAFSKVMPNDFMLNLIEGIIRIAILFAYIVL
                     ISRSKDIERVFQYHGAEHKSIYCYENDLELTVENARKFKRLHPRCGTNFLFIVMAVSI
                     ILFAFFGWPNPILRIFMRIICVPIVAGLSYEVIRVLGKYDNGFTKIIAYPGMMLQYFT
                     TKEPDDEQLEVALEALKAVVD"
     gene            33064..33912
                     /gene="prmC"
                     /locus_tag="L3M60_000529"
     CDS             33064..33912
                     /gene="prmC"
                     /locus_tag="L3M60_000529"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBN6225232.1"
                     /translation="MTIKDIIIKYSDILKDISDTPRLDTELLLQKTLGVDRLYIHLNL
                     NKELTEEQKTEFIGFAEERLNGRPIAYIVENREFMGLDFFVKEGVLIPRPDTETLVEE
                     IIEICREKKDVSILDIGTGSGAITISLAKYIENSKIMSFDISEIALEIAKKNAITNEV
                     DEKIKYINSDLFTAISNSNIKFDIIVSNPPYIKKQDIETLHTQVKDYEPYNALEGGED
                     GLDFYRRITEQGKKYLNKFGILAYEVGHNQAEDVINIMKSNGYKKIYTKKDIQGIDRV
                     VIGYNI"
     gene            33956..35020
                     /gene="prfA"
                     /locus_tag="L3M60_000530"
     CDS             33956..35020
                     /gene="prfA"
                     /locus_tag="L3M60_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBN6225233.1"
                     /translation="MLKKLEVLEDTYKDLSEKIGDPDVINDQKVWQKYIKEHADLEPI
                     VMKYREYKSVLDSIKESKEILQEESDEELRELAKMELAEMEEKVAPLEEEIKILLLPK
                     DPNDDKNVIVEIRGGAGGDEAALFAGDLFRMYSRYAERRRWKIELLSASDTGVGGYKE
                     VSFMIKGKGAYSRLKYESGVHRVQRIPSTESGGRIHTSTSTVAVLPEVEDVEVEINPN
                     DLRIDVFRSSGNGGQSVNTTDSAVRVTHIPTGEVVSCQDGKSQLKNKEQALKILKARL
                     YDKALAEQHKDIAAERKSQVGTGDRSERIRTYNFPQGRISDHRINLTLYKLDAFLDGD
                     IDEMIDALITVDQTEKMTAI"
     gene            35149..35829
                     /locus_tag="L3M60_000531"
     CDS             35149..35829
                     /locus_tag="L3M60_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HBN6225234.1"
                     /translation="MILKVTIIGLLAGVIGTGLGGVISAIFKREVDKYLSFFMGISGG
                     IMLAVVVFDLMKESMDKMGIINTVIFTFVGALITMYIKTKLDMSGNMASGYLIFISIL
                     LHNLPEGLAIGSSFMSTESLGITLAIVIGLHNIPEGLAMALGLVCNKMKLSKVILFTV
                     IAGLPMGLGSFLGVYFGGVFTSLIGVFLATAGGTMMYVVLEEIFPHSKSVYCIIGFLL
                     GTMIVNYI"
     gene            35858..36898
                     /locus_tag="L3M60_000532"
     CDS             35858..36898
                     /locus_tag="L3M60_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBN6225235.1"
                     /translation="MKTIISNIDINNIDYEEIKIQAKLLREGKTVIFPTETVYGLGAN
                     ALDENAVKKIYEAKGRPSDNPLIVHIYEKEEVYDLAKDISDKAKLVIEKLWPGPITII
                     LNKKDIIPYKTSGGLDTVAIRMPSNIIARAIIKEAGIPIAAPSANISGRPSPTKAKHV
                     YEEMNGRVDGIVLGGDSNFGLESTVLDLTEETPMILRPGSITKEVLEELLGEVKLDPS
                     LSKKEDNQKAKAPGMKYKHYSPNADVYIISGKRENVAIKINDMIKSNREKGLKTGVMC
                     TSKNREFYDGEVIDLGNSLEEVASNLFDALIEMDKKSVDVIYSEEFPKTGVGQAIMNR
                     LLKSAGYKVIKA"
     gene            36915..37364
                     /locus_tag="L3M60_000533"
     CDS             36915..37364
                     /locus_tag="L3M60_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HBN6225236.1"
                     /translation="MNILIVCTGNTCRSPMAEAILKKAIKESGRSVEEYSISSAGIST
                     ANGMSASENSIKALKEIGIDLSNHRSKVITKKLIDESDIILTMTKSHKEILLQAVPKC
                     KEKVYTFKGFANKNEEDISDPFGGNLDSYRSTMREIVYSVNEIVKKI"
     gene            37381..37833
                     /gene="rpiB"
                     /locus_tag="L3M60_000534"
     CDS             37381..37833
                     /gene="rpiB"
                     /locus_tag="L3M60_000534"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBN6225237.1"
                     /translation="MKIGLGCDHGGYNLKKEIISYLEGKGIECVDYGTNNATDSVDYP
                     VYGEVVANSVINKDVDYGILCCGTGIGISLAANKVPGIRCAVVSDVFSAKMSKAHNNA
                     NMLSLGERVLGKGLALEIVEAWINTDFEGDRHARRVNMIKSIEEKHNK"
     gene            37869..38498
                     /gene="upp"
                     /locus_tag="L3M60_000535"
     CDS             37869..38498
                     /gene="upp"
                     /locus_tag="L3M60_000535"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBN6225238.1"
                     /translation="MSKVIETNHPLIQHKLTLMRDKNTGSKDFRELLTEIAMLMGYEI
                     TKDIPLKDVEIETPIQKTSSKVVAGKKLAIIPILRAGLGMVDGLVSLMPAAKVGHVGL
                     YRDPETLKPVEYYCKLPQDIGERDIIVVDPMLATGGSAVAAIDLLKSKGAKSIKLANL
                     VAAPEGIAEVQKYHDDVDIYVASVDERLNEHGYIIPGLGDAGDRLFGTK"
     gene            38798..39613
                     /locus_tag="L3M60_000536"
     CDS             38798..39613
                     /locus_tag="L3M60_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBN6225239.1"
                     /translation="MKNCIVVRGAGDLATAVIHKLHNSGFKVVALEIEEPLAIRRQIS
                     FCEAVYEKKVTVEDVECIFCNTEKEIEEALSQRKVALIIDPKGEKLSKLNPIVLVDGI
                     LAKKNLGTTKDMAPLTIALGPGFCAGEDVDVVIETMRGHNLGRTITNGRAEKNTGVPG
                     AIKGFSKERVMHSQHDGIMENVCKIGDIVEKGDLLCYINNYEKKHEVRATISGVLRGL
                     LKDGARVNKNLKILDIDPRKEEVKNAFTISDKARCIAGGVLEAILNNGIYPQN"
     gene            39839..40276
                     /locus_tag="L3M60_000537"
     CDS             39839..40276
                     /locus_tag="L3M60_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HBN6225240.1"
                     /translation="MRPSWDEYFMEIAEVVKKRSTCIRRQVGAVIVRDKQILTTGYNG
                     SPRNLEHCENIGCKRQELNIPSGERHELCRALHAEQNAIIQAAHNGISVDGATLYVTT
                     RPCVLCAKMCINAGILKIVYEGDYPDDMSTELLKEAGIELIKF"
     gene            40461..40688
                     /locus_tag="L3M60_000538"
     CDS             40461..40688
                     /locus_tag="L3M60_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HBN6225241.1"
                     /translation="MSKRNTHTEIAKLFSLISQIGLMIVISILGCTFLGKFLDSKLNT
                     TPVLTIIFLLLGVGGAFSGVYKTLIVYTRRK"
     gene            40693..41079
                     /locus_tag="L3M60_000539"
     CDS             40693..41079
                     /locus_tag="L3M60_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HBN6225242.1"
                     /translation="MDIKLLNQVRSVTKGIVIFDVVLIVLMLITSTLSKPVLLGLIFG
                     SIIAMLNFRLLAISLEKSVTFPAGKAQAYASAQYAIRLFIMAVVLFASATVPHLNIIG
                     TALGLLSTKFVILSKNFINKLKRKEA"
     gene            41082..41786
                     /gene="atpB"
                     /locus_tag="L3M60_000540"
     CDS             41082..41786
                     /gene="atpB"
                     /locus_tag="L3M60_000540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBN6225243.1"
                     /translation="MNQAHYYLIGNFKISETILVSWGIMAFLAIASYFLTRNLKKVPT
                     SKVQIFLEFAVGGLAKMVTDVMGKNTVKRMPYMIPYIGSLFLFFACSNLSPLLGLRSP
                     TTDLDTTAAWAMITFFMIYFAGVKFKGLSYFKELAQPLAIMLPINIIGELARPISLSF
                     RPFGNILGGTIIMGLFYQLLGFISSLIPGLSIPIGQILIPVPLHLYFDLFAGLLQAYI
                     FIMLTMVFISSAGELD"
     gene            41855..42115
                     /gene="atpE"
                     /locus_tag="L3M60_000541"
     CDS             41855..42115
                     /gene="atpE"
                     /locus_tag="L3M60_000541"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HBN6225244.1"
                     /translation="METAIVAAASAIGAGIAVATGIGAGIGQGIAAAKAAEAVGNQPE
                     AKGDITSTLLLGVAIAESSAIYGLVISIILLFVNPFFKYLGM"
     gene            42238..42735
                     /gene="atpF"
                     /locus_tag="L3M60_000542"
     CDS             42238..42735
                     /gene="atpF"
                     /locus_tag="L3M60_000542"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBN6225245.1"
                     /translation="MEKALVGITWEFVFQIVNTFIIFLLLRKLLFKPVLNIIESREND
                     IKNDLAEGEKAKNEGLALKKEYESKINFAKDEGQEIIKQATIRAEQKSDDIVNTAKKD
                     ALDIKEKANKDIEQEKQKVINEIKNDISNIALLAASKVIEKDLDKSKHEELIENFIKE
                     VGEAK"
     gene            42732..43277
                     /locus_tag="L3M60_000543"
     CDS             42732..43277
                     /locus_tag="L3M60_000543"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBN6225246.1"
                     /translation="MINVIANRYAEALFQLGEEENSTDVLFKELEKVVDMMTKVSKDF
                     YKVLKSPLVSKSEKKNLVEIIFSKEVSSNIKNFLKVLVDKDRISYLEDIELAYKELLN
                     KKNNVIAGVAISAIPMSETDIKELEVKLSNKYNKNVTIENVVDKTILGGVLVRIGNEQ
                     IDGTVKTRLDKMKEKLSEVIS"
     gene            43294..44796
                     /locus_tag="L3M60_000544"
     CDS             43294..44796
                     /locus_tag="L3M60_000544"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBN6225247.1"
                     /translation="MNLKPEEISSIIKQQIKNYENKVELTDTGSVLTVGDGIASVYGL
                     EKAMSGELLEFPGEIYGMALNLEEEVVGAVILGDDSEIKEGDIVKRTGRIVEVPVGEA
                     LIGRVVNSLGQPIDGKGPIAYTKTRPVESEAPGIIDRRSVYEPLQTGIKSIDSMIPIG
                     RGQRELIIGDRQTGKTSIVIDTILNQKGKDVICIYVAIGQKRSTIAQLVSSLEKGGAL
                     DYTIVVSATASESAPLQYIAPYAGAAMGEEFMYNGKHVLIVYDDLSKQAVAYREMSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDELGGGSMTALPIIETQAGDVSAYIPTNV
                     ISITDGQIYLQPELFYSGVRPAVDPGISVSRVGGSAQIKAMKKVAGTLKLAYSQYREL
                     AAFSQFGSDLDEDTKKRLAQGERIVEILKQGEHQPIKVENQVMIIYAVINNHLEDIPI
                     DNIARFESELYAFVDNNYPEISRKILGGEDFTHDLTDAINEFKEKFVVEV"
     gene            44824..45702
                     /gene="atpG"
                     /locus_tag="L3M60_000545"
     CDS             44824..45702
                     /gene="atpG"
                     /locus_tag="L3M60_000545"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HBN6225248.1"
                     /translation="MAGAGIKAIKTRMGTVESTKQITKAMELVASSKLRKAKEKAEDS
                     REYFNTLYNTVKDIAQTTRGIRNEFLKEREVKNKCYVVVAGDRGLAGGYNSNVLKESV
                     SHMKGKKETVITVGKKAHEFFSKRNYNIVKDINSVEACGYEDATEISQTIIDLYKKGE
                     VDEVYIAYTKFKSALSQEVEILKLLPLVFEKDTDEQGMEVKEEKQNRSKVQYIPSAEA
                     VLSYIVPQFISGTVYGGILESFASEQGARRTAMESATDNANEMLSNLELSYNRARQSA
                     VTQEITEIVGGVEALK"
     gene            45715..47109
                     /gene="atpD"
                     /locus_tag="L3M60_000546"
     CDS             45715..47109
                     /gene="atpD"
                     /locus_tag="L3M60_000546"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBN6225249.1"
                     /translation="MANVGKVVQIVGAVLDVKFDSEQSLPNLLNALVIKLGDKEIVAE
                     VAQHIGDDTVRCIAMSATDGLVRGMEVVDTGGPISVPVGDETLGRIFNVLGKPVDGKP
                     APKSAPKLPIHRPAPAYDELETTAEILETGIKVVDLLAPYLKGGKIGLFGGAGVGKTV
                     LIQELINNIAKQHGGISVFSGVGERTREGNDLYGEMSESGVINKTALVFGQMNEPPGA
                     RMRVALTGLTMAEHFRDEQGQDVLLFVDNIFRFTQAGSEVSALLGRMPSAVGYQPTLA
                     TEMGALQERITSTKKGSITSVQAVYVPADDLTDPAPATTFSHLDAKTVLSRQISSLGI
                     YPAVDPLESTSRILDPSIVGKEHYEVARGVQSILQRYKELQDIIAILGMDELSDEDKL
                     IVARARKIQRFLSQSFTVAEQFTGNPGQYVPVKETVRGFKEILEGKHDDLPESAFLFV
                     GTIEDAVKKAKGSM"
     gene            47112..47372
                     /gene="atpC"
                     /locus_tag="L3M60_000547"
     CDS             47112..47372
                     /gene="atpC"
                     /locus_tag="L3M60_000547"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HBN6225250.1"
                     /translation="MASEFSLKVVTPNNTFYDGDVEMIVVRTTKGDKGVLKGHIPFVS
                     GIPVGTLKIKKEGSFKEAKIAEGFVNVTQDKTVILTESAEWI"
BASE COUNT        19311 a         5112 c         8826 g        14391 t
ORIGIN      
        1 aagttggtat atataaatag tataaactgt gttgctaaaa tatggtagat attatataat
       61 ataagtggat atgtatatac attatgagtt atataatttt gaatttaata aaatagaaac
      121 gttgatttat gttctgtaat gtgggcacct tggagcatat tgagttagtg gtgcaaccgg
      181 ctatgaaatt gtatttattt atagatacta ttattttcat agcttttttt atttaaaaaa
      241 tggttatata ttagaaataa atttggttag tacgtttaaa taattttact tggattttta
      301 accagatata ttataagcaa gtattaatta aaaagttgag ggggaataaa aatgaagtta
      361 aaaaagaata aaaaaggttt cactttagtg gaattattgg tagtaattgc aattataggt
      421 atattagcag tagtggcagt tccagcttta tttagtaata taaacaaggc taaggtagca
      481 agtgttgagt ctgattatag ttcaattaag agtgcagcat tatcttatta ttcagatact
      541 aataaaatac cagttacacc agatggtcaa actggtttaa atgttttaga gacttatatg
      601 gaatctcttc ctgataaagc tgatataggt ggagaatata aattgattaa agttggtaat
      661 aaattagtat tacagatagg taaagatggt gaaggagtta ccttaacaga agcgcaatca
      721 gcaaaattat tgagtgatat aggtaaagat aaaatatata caggtgttac aggagataat
      781 tttggagagc aattaaaaga tactacaaaa atagataata aagctctata tatagtactt
      841 atagataata ctgtgatgga ttcaacaaaa tagttaaaac aatattttta taaaaatatt
      901 gtaaatttaa tgaaaaatat aatataaaag acaggaagaa aatcttcctg tttttttata
      961 ctattttctt taatgttcaa ttatgaatga tgatataatt gagatagtaa aggagtgaaa
     1021 cctgtggcta aaaaagttag aataggagac aaactggtcg aaaaaggata tataacagaa
     1081 gaccagctta aatgggcatt atcagaacaa aaaaacagtg gaaaaagact aggggaattt
     1141 ctagtacaag aagggcttat agatagtaac ttattaataa gtgtattaaa agaactctta
     1201 gatatagaaa gcatcttttt agaaggaaca gaaatagata cattagctac aaagatggtt
     1261 ccagaaaata tatgcaaaag atacactgta tttccattta aaattgatgg aaataaaata
     1321 tgtctagcca tgtcagaccc acaagacaga gaagcagttc aagatgtaag acgtatgtct
     1381 ggtaaagacg tagagatatt tatatcaagc acagaagata taaacaaagc aataggtcat
     1441 gcttatgcac attcagaaat aaataaggca atgactgagt acaacaaaaa tagaactggt
     1501 ggagtaagag aaacagtaat acttgaagaa gatgtaaatg ctgctccaat agtacgatta
     1561 gtaaataaca tactagaaaa tgctgttaga atggaagcaa gtgatataca tatagaacaa
     1621 agtgaaaatt acatgcgtgt taggtttagg atagatggaa tgttaagaga atatatgagg
     1681 atgaactcag caccatataa ggcagtaatc agccgtatca agataatgtc agatataaat
     1741 atatctgaaa agagaatacc acaggatggt agaatctatt taaaagtaga taataaaccc
     1801 atagacttta gggtttctac aatgccaact aacagagatg aaaaaatagc catgagggtt
     1861 cttgataaga gtaattttat ggttagtaaa gaagtactag gaatagatga gcatggctct
     1921 aagatatatg atgaattaat aaacactcct tatggtctta tattggttgt tggccctact
     1981 ggaagtggaa aaactactac attgtactca atgctaaatc aactaaatac agaaaataga
     2041 aacctattaa caatagaaga ccctatagag tatgaattac ctggtgtaaa tcaatctcaa
     2101 ataaatgaaa aggcagggct taccttcgct agtggtctta gagcattcat gcgtcaagac
     2161 ccagatataa taatggttgg agagataaga gatactgaaa cagcagaaat agcaattaga
     2221 gcttctctta cagggcattt agtcttaagt acattacatg caaatacagc agttggagca
     2281 atttcacgtc ttttagatat ggatgtggaa agttttctga taacttcatc tgttttaggt
     2341 gtaatatctc aaagacttac acgaaaaata tgtgagcatt gtaaggtttc atatgaagca
     2401 gatattggag aaaaaaaggc tcttggtatt gatgtaaatg aatcagtaac tatttataga
     2461 ggaaaaggtt gtgaaaggtg taataatact ggatataaag gacgtctagg tatatttgaa
     2521 atgcttgaaa ttactccaga aataaaagag ttaatagatt catctgcaaa tcaaagagaa
     2581 attttaaaga tggctagaaa acaaggtatg gttagcttaa aagaggatat agttaaaaag
     2641 gttttaaatg gaaagacaac agtagaagaa atgattagaa taattttgat gactgattaa
     2701 aggatgatat tatgaaaagt tttaaataca cagtaataaa agaggacgga aaaaagcaaa
     2761 ctgatgtgat tgaagccaac gattttaatg aagctagaaa tctccttaga aagagaaatc
     2821 ttagagttat tgaaatcaaa gaaagtaaaa ataaaaatat aagattatct agtagaaaga
     2881 gaaaaaaaga cttaggagct gaccagataa gccacttttg tagacagttt gctattatag
     2941 tatcttctgg tataaatagt atttctggat tagaaacatt agctagaagg tcaactaaca
     3001 ttacattaag agaagaaatt aatcgtatag tggcagaaat aaaagtaggt tctactattg
     3061 cagattcaat gctttcatca aaatcaaagt ttccaaagtt acttggtgct atggtagcta
     3121 caggtgaagc tacaggtaaa ttagatgaag tattaaaaag tatggcttca ttttatgcaa
     3181 gtgaacacag ggttaaacaa aagcttagaa atgctgcaac atatccatta atagttttga
     3241 tttcttcatt tataatgatt tttatattta cagcatttat ggtgcctaag atgattaatt
     3301 caattacaac agtaggagca actcttccac ttataacaag aattgttatg gggtttggta
     3361 tgtttatgaa gaagttttgg ataatagtac ttttagttat tatattattt atctatcagt
     3421 ttaaaaagta tctaaaaacg ccaattggaa gagctcataa agatagagtt ataaacaaga
     3481 tacctgtttt aggaaaagga ataaactgta tggtagctac acggttttca agagcattgt
     3541 atctatttgt atcaacagga tatccattgg ttcaagggct tgattacatt atagatagtg
     3601 taaataacac catggcagaa aagattttag tatcagctaa agacggaata acaagaggag
     3661 aaggtttagc agaaaattta gagagatata catattttga ctctgtattg gtacagatga
     3721 tagctatagg ggagcagaca ggggaattag agaatataag taaacagatg gccgaattct
     3781 atgagtatga atccgagatt tatttaaata ggatggcatc aatgatagag cctgttttaa
     3841 taattgcagt tggtattata gttgcgatac tggttgtatc aatatttatg ccaatgctta
     3901 gtatatatga tgctatgtag gagggaaatg acaaatgaac aaggtttatg tatcatatta
     3961 tggcgattct atttccatag ttgagggcag atataaacac aataaggata agttttatat
     4021 aaaaaatctt aatgtgtttt caatagagga tattgcacaa gatttttcta aggaaaagta
     4081 tgctctattg agatacgcat tagaaaatgt taagttaaaa tcaagacatg tagttttttg
     4141 tttaaataca agagatgtaa ttttaaaacc ccaaaaactt cctaatatgg ataaaaagga
     4201 tttagacgga tttatgaagc tagagatgga cgaaatgatg gctttggagt cagatgaata
     4261 tgtattttcg tatgaagtat ctagtgaaac acctgataat tcagatggcg atgaatcaag
     4321 cttaaatctt atcattggag ctatacaaaa ggaagaggta aatgcaattg tagagataat
     4381 acatgaattt gatttaatat tagatagaat agatacatta tcaacagctt acttaagaat
     4441 gcttaaaaat ttagattaca atgatataat ggtaactaat ataagtgaca atagcacaat
     4501 tataaatata tacaagaaag atactttatt tattagtgat aatataccta taaagataac
     4561 tgctaaagat atggacatgc aattattaaa tgtagtagaa gaatcaaaag gtcttatgaa
     4621 ttactattcg tcaagaaact ttggtaaggt tacagatact atattaatat taggaaaaag
     4681 gtattccaat aaattaattt atgaaacttt tagctctgca ttttcaagct atgtttctca
     4741 aggtcttact gagataatgg atatatctga tatgataact ggagatatag atgaagatga
     4801 tataaattca attgtagagc ttttaggaag tatgatagaa cctaaaggga aaagagattt
     4861 tgaaaatata aactttttac caatggatat acttagaaaa cagctaaaag ctaaacagat
     4921 gaaaaacata ctgaaaattg ctccagtagt tattttgatt cttgctatac cttatttatg
     4981 tttaattttt tcaaataatt tagcaaacaa ggaacttgaa aatgttaatg ctgaaataag
     5041 aaatattgaa tctgcatatt atcagattgg aaatatagaa aaaatgatac agagtaaaag
     5101 cgaagaaata aaggtatatg atatgttaat tggaaaaaag gtaaagtggg aaaatttgtt
     5161 ggatgatata gataaaaata ctccaagtaa agttgagcta acaagtttat caacgactta
     5221 tcaaaatctt ggagctaata ataaagctga taactcaagc agtaataaga ctacaacaga
     5281 taaatcaagt aacaatgaaa caaaatcaaa tacgaaaagt gatagtaatg gagataaaac
     5341 aaataatgat ttaaatgaag ataagactac aaatgtgtca gaagcattaa ctggaaatag
     5401 tgagactaat agtaataaca cagataaaga tgataaaact ccattgtatg ataagatacc
     5461 aaatgttata aacatagaag gaaatgctaa agattcctct tatgtaggtc aatttttata
     5521 taaattaaag ggattgtatt atttcaagga tgttaaatta catagcataa aaagtaatgc
     5581 agaagattct acctatacat ttagtatgac attatacctg aaagaaggtg tattaactaa
     5641 tgaataaaga tatatttaaa aaagatataa aagatatatt taaaatggaa gttagtctaa
     5701 aaggggttct tataaaggtt ataattttat tatctatttt atcatatggt tactttggac
     5761 tttaccctca atatcagaaa tatattgtta caaaaaattc tgtaaaacaa gctactgaaa
     5821 aattaaatat atataaagag aaaactgcac ttatgccaaa atatgaaaaa caacttgaag
     5881 aagttgaaag tgagttaaaa gaaaaaagca aaaatctttt atatgatatg caagatggtc
     5941 tttttttaat aggtttagat aaaaaaatga aggcttataa tattgactta actagttatg
     6001 acgttggaaa tgttagagaa tacgaacatt tttatggtgt atcaacaaca ataagtgtta
     6061 gaggtgatta tagaaatgtc aaaaaattaa tggcttattt agaagaacaa aaaaatgtta
     6121 ctcaaatact agattataca atgacttcat atatagaacc agaaaaagaa gaggaagaaa
     6181 caacaaacaa taaggattta attaaaaatg tgaacataaa taaagataca aaaatatact
     6241 gggtggaagg cgaaaataag tggcatttat ataaggattg tccattgata aaagagggag
     6301 agattctaaa aaatgacaaa cttttagata ggtctactag ttatctatgt aatacttgtg
     6361 tacttagagc tgcaacagaa aaagaagcaa aaaaagaagt cgtagctcca aaggcagaag
     6421 gtgtagtaga tgctacattt aaatttataa tgtacactaa agagaatact acaatgatac
     6481 ttgatacaga taatccatct tcatggagac cgggaaaata taatccattt aagtctagtg
     6541 cgaaataaag ctaagtaagc tttaaagata ggtgatgagt ttgtacaaaa ataatgaaaa
     6601 aggtcttacc ttattagaag taataatagc tgtttttata ctgacaatag ttttaagcat
     6661 ttcttataag gtgtttaatg gtataacatc agcagtaaaa aagcaacaga ttattacaga
     6721 tgctcaagta aatattaacc taattaataa gtatctaaat agagatttgg aaaactgtaa
     6781 agagctaact aaaactggtt caggtagtaa ttatgaatat aatatagaga cgccagataa
     6841 tattgtaaaa tatgaagttt ccatagaaac taaaaaaaat acagaggtat attctgtaac
     6901 aagaatcgaa aaaaatacaa ttgatacaga aaatgaagtt agagaagaga taatctataa
     6961 tcaaccacta gttcaaaaca ataaagaaat gaaagaaaca ccatttaaaa tagaaaagca
     7021 aactgataaa tctatttata ctgtaagtat attctataat gaatcagttc aagaaggtca
     7081 taaaaatagc aatttaaata acaagactta tacatttgat gttatgtcga gaataggata
     7141 ataagaattt attaaaaata agtaggttga taaaatatga gtaaaaagaa tagtaaaaga
     7201 ggattttcct taattgaagt gttagtagct atggctatta tgggaattat actatttgcg
     7261 ttttttaata ttatcaatac taataataaa gcaaatatta aaaatgacac agatataaat
     7321 tccttaaact atgtccaaag tgaaatagaa aatctaagag agaagataaa aagtggagaa
     7381 tttgattttg atagtttaga taaaatggaa gatgggactg tcgtatatga aaaattaata
     7441 gataagtcaa aaaaaattgt gtatgataaa gttcttagtg agggtaacgt gagcttatat
     7501 gatactccat atgaaaagat tacaacaata aaagatgaag atggtaattt aatcgataaa
     7561 gaaaatatta caaataagat aaagactatt gtagaagata aaagtggtca gatatataaa
     7621 atagctgtaa ctgggaaaag catgaatgac tattcaagta aaaaagaagt taagattgta
     7681 actgaaatat ttaaagataa ataattttac ttgggggtga gattttgagg aagtggaata
     7741 aatttaaaag tgaaagaggg gcagctttag ttttagttct ggttgtagta gcattacttt
     7801 ctattgttgg attgatgttt tcaaatcaga tagccaatag aataaaatct acaaagacta
     7861 ctaatgaggg tatacaagca aagtatttag cagagacatg tgtggaaaat agtattgata
     7921 aagcatatga aaaattgtat gatgagcttg agaaaatgga taatgagttt aaaagtgaaa
     7981 atcaacaaaa atccatatct aggtcaaaat taagaaatat atcagatgaa gattttaata
     8041 atcaaaatga agaaaatctt gaagtagaaa gactaggata tatgaataat ataaatttct
     8101 atttaaacaa agcttcctca gatttggaaa aagctagtat agaattgaaa aaattatatg
     8161 atttagatat gttggattac agagacattg aatatgtaga tgaaaatatt atttcacata
     8221 gaaattctat tttagatatt tgtaagaatt atacttctgg agatattagt aaaataaatg
     8281 aatatattct ggatgaagat ataaattcaa ctacgttagt tgaagcaaag ttagtaaata
     8341 atgatattct tttaaaaatg tttttagaag aaaataaaat tgaaaatgaa catttaaatt
     8401 cagcgttttc atacacttat aaggcattag ataaaatttc tttggcgatg caaaatatga
     8461 ttgagtatag gcatacattt catatagatg agcaaaaagt agaagtatct aatgggattc
     8521 caaattctca acaatattat gaattaatac aaaatcctat aataaatagt aaagagtata
     8581 gctggaatag taaatgggat acattagaaa atctattgga aactttacca aatcaaactc
     8641 aaggttttaa ttctttaaga gttcatttga gaaataatgt aagaaaattt gaggagttaa
     8701 gcgataatat atctagtggt aaaaaaaata cagcaaaaaa tttcttaaag tataaagagt
     8761 tattgtatga aatatcagac caatgtaatc aactaaaaag tatgtcttat gagaaaatac
     8821 ctgttaaata tgataacatg gccttgataa caacttttga ctatatacaa aatgagcttt
     8881 tagcagagat aaaatgtaga ttaaaagaat taaaacctca agaagttgat aaaacagaag
     8941 atattactat aaaaatacct ttttataagg ctgactacga catgacaaaa gaaggatggc
     9001 ctaaattaaa agaaaatggt tcaggggcgg agttaagctt aatggtaact ggtgataaag
     9061 atggaataaa agaagtggaa gttacagata gtaaaaaaaa tattattggt ttaggtgttg
     9121 aagaaaattc aaattcaaag tataaagttg aagctattat aaattttaat ctaaatatag
     9181 atacaaatat agtcggaaat tatgatataa aagataaaat tttgattaat catgacataa
     9241 gttcatacaa aaaagtaaac tagatataat aagtaactta gtgtgaataa ataataaaat
     9301 aattttatta tttactacta aaaaaataag taaactaaaa aaagatatgg agattaaata
     9361 ttatgtatat ttatgattta ttagagcaag gtataagact aaacgcatca gatatacaca
     9421 taacagttgg aacgaatcct gtagctagag taaaaggtgg atttgtaaaa ctaagtgaac
     9481 agatattgac accagaagta acaatgcaaa tggcaaaaga catagcagga gaatctatgt
     9541 ttaaagtaat aaaagaacat ggagaagctg atttttcagc ttctctaaaa acaggggaaa
     9601 gatttagggt aaatgcatat aggcaaaaag gaaattacgc aatagcaata aggactataa
     9661 cagcagaaat tccaacattt gaaaaattag gtcttccaga aaatataaaa tcttttactg
     9721 aaaagcataa aggattagtt ttagttacag gtccaacagg aagtggtaaa agtacaacac
     9781 ttgcaagtat gataaacata ataaatgaaa aacaacaaaa acatataatt acacttgaag
     9841 acccgataga atatgtacat catcataaac aaagtttagt aaatcaaaga gaagtaggaa
     9901 ctgatacaga aagttttcat tcagccctta gagctatttt gcgtcaggac ccagatgtaa
     9961 tactaatagg agagatgaga gaccctgaaa ctgtttcaat agcattaaca gcagcagaaa
    10021 caggtcactt agtattttca acactacata cagtaggggc agctaagaca attgatagaa
    10081 tagtagacat gtttcaacca tcccaacagc aacaaataaa aacacagtta tctacagtct
    10141 gtgaaggtgt ggtatcacag cagttacttc ctacagcaga tggaaaaggc agaatagcag
    10201 caattgaatt gatgtttgct actccagcaa taaaaaatct gataagggaa ggcaaaacat
    10261 atcaaattcc aaacatgata caaactggag ttaaatcagg tatgaaaact atggaccaag
    10321 atttaatgga actttataaa aatggaaaga ttacaaaaga tatggcactt agtaggtgta
    10381 ctgaccaaga gtttatgaca agaatgatag gtggagtaaa ctataatgga tattataatc
    10441 gttaatctat atgtatttat agtgggtata gtgtttggaa gcttctttaa cgtctgcatt
    10501 tttaggattc cagaaggtat atctatttct agacctccct cccattgtat gagttgtaat
    10561 actagactaa agccaataga cttaatacca gtgctaagct atttaataag tggcagaaag
    10621 tgtagatatt gttatgagaa aatttctagt agatatgcta tagtagaatt aattacagga
    10681 attttatttt taatagtatt tatgttatat ggaatatcta catcaactgt ttactatttg
    10741 gtacttgtat ctttactgat tataattaca tttatagata ttgaccactt tataatacca
    10801 gatgaaatac tgatttttgg agctattttt gctataattt ttaacctttt atttaaagaa
    10861 attccagtta aggattcaat tataggtttt tttatttgtg gaggaagtgt ctggcttata
    10921 gttcttttaa ttgaatttgt aataaaaaaa gagtgtatgg gtggaggaga tattaaacta
    10981 tttgcaatgc taggtcttta catgggtgtt aaaaatagtc ttttgacagc tttactaagc
    11041 gtctatgtgg gagcagtcta tggaatctgt gtaataatta taagtaagac aaaaggaaag
    11101 gaatataatt ctattatacc atatggtcct tttatatctg ttggagcatt aataacaatt
    11161 ttatgtggaa aacaattact cgaattttat ttttctatga ttacatagat aatagtcaag
    11221 ctaaatcaag ttttaattta aaaaaatgaa ttaaagtatt caataaggac aatattatta
    11281 ttaaccctat tactaaagtg ctacaaacta tggagtatat aataataatt tccttatttt
    11341 ttgcacattt gataaataaa tctctatgtg ttttttgtga tagagaaaag aaaaacttat
    11401 ataatacgtt ttttatttac ataataactg tagttttatc tgtattaata tatctaaaat
    11461 ttggaattag tgtagaatgt gtacaatata tggttttaat tccatttcta ataattatat
    11521 ccattgtaga ttactacaca atatatgtat atgatataac aatagttagt ggtataatag
    11581 ttcaaggtat aatttttttg gtcttttgca atatagttca atttgattat ataaatcata
    11641 tatttggact agtgataggc tttattatac cttatattat tgctaaatta acaaaaggat
    11701 taggctatgg agatgtaggt ctctatagct tatgttgctt tgcattggga aataattatg
    11761 ggttttatgt gatagtttta tcatttattt tagcgttttt gttttttatt attttataca
    11821 taactaaaat atttaaagat atagatataa aaaagcaaat tccttttaca ccttttattt
    11881 ccttagcaac aattttaata atgtttacag agtataatat atttaatttt tattatagtt
    11941 tgttatacaa atttatataa caggaggtaa gaaatgtacg tagtagtagg gttaggtaat
    12001 ccaggaaaga agtatgagaa gactagacat aacgttggat ttgatgtgat agatattctt
    12061 gctaaagaat acaatattag tgttactaaa ataaaacata aagctttaat tggtgaaggg
    12121 agagtaggta ctgaaaaagt attattagtt aaaccacaaa cttatatgaa tctaagtgga
    12181 gaaacattaa tagatatata taaatattac aaggttgatt taagtaatat agtagtagta
    12241 tatgatgata tagatttaga agttggaaag atacgtataa gaaaaaaagg aagtggggga
    12301 actcataatg gcatgaaatc tataactaag tgcctaggtt caaatgactt tccaagagta
    12361 agagtaggtg tatctaagcc agaagttggt caagacttag cagattttgt actttctaga
    12421 tttagaaaag aagaatctga taacataaat gaagcattag aaaaagcagc atatgctatt
    12481 gactcaataa taagagaaaa tatagatatg tctatgaaca aatacaatgg atagtgagag
    12541 ggagatttaa atgaatgatg tttttttata tcctttgcaa aattcaaaag agtataaaga
    12601 tataataaat tgtataaaaa atacaaaagg ttcattattg gttaatggac ttttaccagt
    12661 acaaaaacct catatatctt attctatatt taatgattta tcacgacaaa tgatttttat
    12721 aactagtagt gatttagaag caaagaaagt atatgaagat ttaagttttt atattgaaga
    12781 taaggttgaa tatttaggtt ttcaagatat ttatttttat cacttggatg caaaggacag
    12841 aaatgaagaa gcaaagaaat taaaggtatt gttaaaacta gcaaataaag aaaaaataat
    12901 tttagtaact tctattgagg cagttttaag aaaatatata ccaaaacaag ttttgctcga
    12961 tagtgtatct cattataagg tgggagataa cttagactta gaaaggttaa ctgaaaaatt
    13021 agtttcattg ggatatgaaa gagtttcaaa aatagagggg tttggtcaat ttagtataag
    13081 aggtggaatt attgatgtat tttcacttga atatacaaat ccaattcgta tggaattatt
    13141 tgatgatgag atagattcca tacgaacttt tgacgtatat tcacaaaaat caattgataa
    13201 attgcaacaa ttctctataa ctccatctag ggaatttatt tatccagaaa aaactacaga
    13261 tgcattagtt aaactaaaaa aagaaacaac taaaaatact gatgaaggta catttcaaaa
    13321 tatagactat atatcgagta agacttattt tgaaggtgta gaaaactata tagattatat
    13381 ttatccagaa gaaaacaaaa gtatatttac ctatctagca gatgatgcaa tagtatttat
    13441 taacgatata acaagactta aagaaagatg cgaaaactat ataaatgagt ttagagaaaa
    13501 ttataaacta aatcttgaaa gaggtttggc tattaaaaac caaggtaaat tgttatatca
    13561 ctatacagat ttagagtact tagtaaaaga caagtctgtg gtattaaact ctctattgcc
    13621 aaagtctata aataattttt ctataaaatc tataataaat tttgaaagta gagaagttcc
    13681 tacctttaat ggaaaggtag atttgcttgt agaagaatta aatcggctga aatataatgg
    13741 atacaaaata attttagcaa caaatacctt agaaagagca aataaactag gaaaagattt
    13801 attggacaaa ggcttagaaa caaccatttc taaagataga gatattgaga ttaaatcttc
    13861 acaagttata attgtacctg cacacataaa cagtggtttt cagtataaat ctataaagtt
    13921 tgttgttata acagataatg aaatgatagg tgtttataaa agagcatcta aaactaccaa
    13981 taaaaaagtt aaaaaaggaa aaaaaataga atcattttta gatttaagtg ttggagacta
    14041 tgtagtgcat gaaaatagtg gtgtaggtag atacacagga attgaacaaa ttacagtcaa
    14101 tgctattaaa aaagattata tgaaaatagt atatcaaggt ggagataatc tatatgtacc
    14161 tatagaccag atggacaaag tacaaaaata tattggtgca gaagtagaaa aagttaagtt
    14221 aaataagctt ggaacaaatg agtggacaaa agcaaaagca aaagttaaaa aagaaattga
    14281 agatatgaca aaagatttga ttgagcttta tgcgaaacgt gaaaaaatac aaggttataa
    14341 gttttcaaaa gacacacctt ggcaggcaga atttgaatct ttgtttccat atcaagaaac
    14401 agaggaccaa ttaaaggcga tagaagaaac taaaaaagat atggaatcaa gtaaggtaat
    14461 ggatagacta gtatgtggag acgttggtta tggaaaaaca gaagtagcta ttagaagtat
    14521 ttttaaagct tgtatggacc aaaaacaagt tgctgtattg gttccaacaa caatattggc
    14581 tcaacaacat tacaatacat tcaaagagag gtttgaaaat tatcctttga gagtagaagt
    14641 tttaagtaga tttaaaacac caaaacaaca aaaacagatt attgaagatg ctaaaaaagg
    14701 attagtagat attttgattg gaacacatag aataatatcc aaagatataa atttaccaaa
    14761 cttaggtctt gtagtcattg atgaagagca aagatttgga gtaaaacata aagaagcttt
    14821 aaagaaaatt aaatctacag tagatgtact tacactttca gcaactccta taccaagaac
    14881 attacatatg tctttaagtg gaataagaga catgagtgtt atagaagaac ctccacaaga
    14941 aagacatcct gtaataacat atgttacaga gagtaaggaa agcgtaattc aagatgagat
    15001 agagagagag ttaagtagag gtggacaggt attttttgta tacaatagag ttgaacatat
    15061 tgaagagatg gcaagtatga ttcaaaaact cgtaccagat gctagagtgg ctgttgcaca
    15121 tggaagaatg acttctaaat cattagaaaa cataatccta ggatttttaa ataaagatta
    15181 tgatgtacta gtctgtacta caataattga aactggtatg gatatatcca atgcaaatac
    15241 aatgattata tatgatgcag ataaaatggg cttggcgcaa ctttaccaat tgagaggtag
    15301 ggttggaaga agttcaagac aaggatatgc atatctttta tatgaaaaag ataaaactct
    15361 aagtgagata gcagagaaga gattaaaagc tatcagagaa tttacagagt ttggttctgg
    15421 ttttaaaata gcaatgagag accttgaaat aagaggcgct ggaaatattt taggttctca
    15481 acaacatggt catatggcag ttataggtta tgatttgtat gttaaaatgc ttaatgatgc
    15541 aatcaaaaaa gttaaaggag aacctattgt agaggaaatt gatgttgaaa tagatttaag
    15601 tgtaaatgca tacattccag ataattatat caaagatgag ttaattaaaa ttgaaatgta
    15661 taagaaaata gcttctattg aaaataaaga agacatgttg gatatacaag aagaacttga
    15721 ggatagattc tctgatatac ctaaaccagt ccagacactt cttacaatag cttatataaa
    15781 atcgttatgt aagatattga aaatagaaaa gataagacaa ttaaaagatg aaatattatt
    15841 agttccaata acaaagtata gaactaaaca gaaaataggt tataatatag ttacagaatt
    15901 agaagaatta ttagaaaaaa tgtgcaaggt aaaataactt gtaaatttaa gaagggatgt
    15961 tttaaatttg aaaaaggtaa taacattggt aatagctatg attttggtag tatctgtgac
    16021 tgcttgcaat tctagtaaag gagaaactgt ggcaacagtt gaaggtacaa agatatcatc
    16081 agatgaattt aaaaaaacta tagctcttta taaggattct atggagcaaa cttatggaaa
    16141 agatatatgg gataaggaag ttgaaaaggg tgtaaaatac aaggacaagt ttaaagattt
    16201 aattcttgac caacttataa ctacagaagt aatatattct caagctaaaa aagataactt
    16261 acttcctaaa aaggaggatg ttgaaaaaag ctttaaagaa ttaaaagatg ctatggggaa
    16321 agatgaaaaa tataaagaac agcttaagaa attagggata gatgatgagt ttttaagaga
    16381 ccaacaagag aaagatttag caatgcaaaa ctatcaaagt aactttacta aaaagactaa
    16441 aatatcagat gaagaaatga aaaaatacta tgatacacat aaagatgaat ttaaaaaaga
    16501 tgaggtagaa gcttctcata ttttattgaa aacagtagat gacaataaca agccactttc
    16561 agctaaggaa aaagctgaag ctaagaaaaa agctgaggag gctcttaaag aagttaaatc
    16621 aggtgaggac tttgcaaaag tagcaaaaaa atattcacaa gatgcttcag caagtgatgg
    16681 tggtaaatta ggattctttt caagggggca aatggttgct gaatttgaag acgctgcatt
    16741 ttctatgaaa aagggtgaag tatctgattt agttgaaact caatatggat accacataat
    16801 taaagtaaca gacagaataa atgagcaaac atcatttgaa gatgctaaag aaactataaa
    16861 agaccaacta ttaaaaaata aatatcaaga gcaaatagaa aaattaacta aagaagctaa
    16921 agtagaaaaa gatgaaaaag taataaataa aatcacaatc taagacctaa aaaagagtaa
    16981 tatgcttaaa aaagctatat ctggataaga tatagctttt tttgtatatt tttcttaaca
    17041 atggtaaaaa taaacttaaa gtaatttatg gagggattaa aaaatatgaa agcaacaggt
    17101 atagttagaa gaatagatga tcttggaagg gtagttatcc caaaagagat aagaaagaca
    17161 ttaagaatta gagaaggaga ccctttagag atattcacag caaaagatgg agaagtcata
    17221 cttaaaaaat actctccaat aggtgaatta aatgaatttt cgcaagaata tgctgaaaca
    17281 ttagctgaag tactaggata tggagtgttg ataacagact tagattctat aatagctgtg
    17341 tctaagcttc ctaaaaaaga ttataaagaa aaaagtataa gtgatgagtt agaagcaata
    17401 ataagtgaaa gaacagtaaa gtattcaaaa gataaaaaca ttttaccatt attcaaagaa
    17461 gaccaaaaag aatatactag tcaagttatc atgcctataa ttagtgcatc tggtgactgt
    17521 ataggttcaa ttgtagttgt ttcaaaggac aaagatacaa ttgcagaaag tgaagaaaaa
    17581 tctttaaaaa ttgctgctaa ctttttagga aaacaagttc aataatttta tcataaagac
    17641 cttttacatg aaggtctttt gaagtttttg tgtaaaatat tgaacatagc attaaaaata
    17701 tgttatatta aagtttgtta tataaacagt gtggaggtta tgttatgaac aataacaaaa
    17761 ataaagactc ctttctaaaa ggagccctta tacttgggct agcaggtgta atcgtaaaaa
    17821 taatgggtgg attttttagg attccccttg gaaacatgat tggttcagaa gggatgggat
    17881 actatcaggc tgcttatcct gtgtatactc tatttttaac attagctact gctggatttc
    17941 caacagctgt ggctaagttg gtttcggaaa aagttgccat tggaaatttt aaaggtgcaa
    18001 ataagatatt taaggtatca catacagttt tatttattac tggtataata tcattttgta
    18061 tattattttt tggagccgac tacatagtaa caaacataat gaaaaatcca ggtgcgttat
    18121 actctatgaa agctattgct ccagcattac tatttgttcc agcaatgtct gcatatagag
    18181 gttattttca agggagacaa gatatgacta aaattgctgt gtctcaagtt gcagagcaat
    18241 tttttagagt tgtattaggt cttactttgg cttacttgct tatgaagagt ctaggtcaga
    18301 cttttggtgc agcgggagct atatcaggag ctacaatagg ttcaatagca tctatgcttt
    18361 acttaatgtt tgcttatatg ctagggagaa aagagcgtag agcagagata gatgcaagcc
    18421 aaagatttaa agatgagaga gtgtcttata tttttaaaaa gcttttaact gttgccattc
    18481 ctataacaat aggagcatca gtaatgccac ttgtaaatat gatagataat gttattgtaa
    18541 taagaagact tatggaagca ggctttacat acaaagtggc aaattcaatg tttggtcaat
    18601 taacaggtat ggcaatggcc acaataaatt taccagcagt cataacaact gcaatgagta
    18661 tgagcttagt tccagctatt tctaaagcat atgcattagg aaataagtca aaagctagaa
    18721 aagatactaa gagtgcagtt aaggtaactt tacttatagt attgccttgt gcgtttggta
    18781 tggcttcttt agcaatacca ataatggggc ttttatttcc acatgaacca tcttctgtag
    18841 gaactatttt gtttactctt actccatgtg tattattttt aggattaata cagacattga
    18901 ctggtataat tcaaggaatg ggaaaaccaa tagtacctgt aattgcttta tgtgttggta
    18961 tgctatgtaa aattgtaata agttatactt taacaggtat cccagatatt aatgtactgg
    19021 gttcagcctt tggtacagtt acagcgtact ttgtggcagc catgattaac ttactatatg
    19081 ttaaaaaaca catgaatgta aacttttcta aaaaagaatt tataataaag ccatttataa
    19141 cagttatgac aatgtttata atggttaagt tgagttatgg tgctttagtt ggattcttag
    19201 gaaacagcat atccacaata attgcaatat gtatcggtgg aattgtatat gtagtagtaa
    19261 tacttggaat aggtggaatt aaaaaagaag agattctaac tatgccaaaa ggtgataaat
    19321 tatataaatt gttaaaaaaa gttaaactta taagataata taggaatata tatgcaaata
    19381 agggaaatac tatacaaata gtaaatctcc ttatttgtat agtaatataa aaataatgtg
    19441 aaatttgcta ataggagtga tgttatgggt aagataagta ttgtaggtct tggaccagga
    19501 gactattctc ttataagcca aggtgtgtta gaatctttaa aaaatagccc taaagttttt
    19561 tttagaacag agaaacatcc aataattgat aaacttaaag aaactataca atacacttct
    19621 ttagactact tttatgaaaa agaggaaaac tttgacaatg tttatttgaa aatatctgaa
    19681 tttatcatag aagaatctgc aaagggtgat ttagtatatg ctgttcctgg tcatccaaga
    19741 gtcgcagaaa agactgtaag tataatagag agattggcca atgaaaagca catagcagta
    19801 gaaatagtgc cttctatgag ctttgtagac gctatgttta actatttggc tatagaccca
    19861 tctgagggat ttaaactggt agatgctttt gaaatagata attcatatat agacttggat
    19921 acaagtatga taataactca ggtatatgat aaatttatag catctaatgt aaaattaaaa
    19981 cttatggaat attatgacta taatcaagaa gtatgtatag taaatggagc aggtgtcaaa
    20041 gatttagaaa ataaaaagtt tataaagctt tgtgaattag acagaaatga aaactttttt
    20101 gactatttaa ctagcttata tatacccaaa agttctaaaa agatgtataa tacagtacat
    20161 gacttagaaa taatagttaa tacacttaga agcccatctg gatgtgaatg ggataaaaaa
    20221 caaacacatc aatcattaaa aaattctgtt atagaggaag catatgaact ttgtaatgca
    20281 attgataata atgatataga tgaaatggta gaagagttag gagatgttct tttacaggtt
    20341 atattccatt gtcagatagg aaatgaggag ggctactttg atttaaaaga agtagtaaat
    20401 ggaatatgta aaaaattaat tcatagacat cctcatatat ttagtggtgc aaaactagat
    20461 atgtctaagt ttgaaaaaac ttgggaagaa ttaaagaaag aagagaaagg cgaaagtact
    20521 ataacagatg gccttagaag gataccaaaa catttaccag ctcttatgaa ggctagtaaa
    20581 gttcaacata aagcagcact tgtcggcttt gattgggaca atgtagaaga tgtatttaaa
    20641 aaaattgaag aagaatatca ggaattactt gacgaacaca gggtcgggaa tataaaatac
    20701 ataaaggaag agctaggcga tttattattt tcgatagtaa atctagctag atttttagac
    20761 atagactcag gagaagcctt gaactgtact acagaaaaat tcataaatag atttgagttt
    20821 atagaaaaaa gtgcaatgaa cttgaataaa aaattagaag acatgacact tgaagagatg
    20881 gatgagtttt ggaatcaggc taaaaaacaa taaaattgtg gaaggaaatt tgtcaaaaat
    20941 ctagaacttt tatcacaaag atagaagtct atattataaa catttttagg aggtaaatat
    21001 cgtgaataaa gctgaattag tatcaaagat ggcagaaaaa agtggattaa caaagaagga
    21061 agcagaagct gcgttaaacg catttatgag ttctgttcaa gatgcactag taaataatga
    21121 aaaagttcaa ttagttggat ttggaacatt tgagacaaga gaaagagctg ctagacaagg
    21181 aagaaatcca agagatccag agcaagttat agatatacca gcttctaaag caccagtttt
    21241 caaagctgga aaaggattaa aggatataat aaatggataa ttacaaatct tgtgaaaaag
    21301 tgtggataaa tttgccacac tttttctata gagccaagga aggtgataat tatgagatta
    21361 gataaatttt taaaagtatc taggattata aaaagaagaa ctgttgctaa agatgcatgt
    21421 gataaaggga ttgtcactat aaatggaaaa gttgctaaat cttcttcaga agtgaatatt
    21481 ggagacacat tagaaataca atttggagag aagaagatga agtttaagat aaatgaagta
    21541 aaagagcatg tattaaaaaa tgatgcaaaa gaaatgtacg aaattttaga ataaaattaa
    21601 tagtcctcta gtatattaat agtagtatta ttgaaatatt tactgggggg atattatgga
    21661 acagaacata actttgaaag atagatcaaa gctggttatt tcgggagttg agcacatata
    21721 ctcttttaat gacaaaagag tagagctcaa aacttctgta ggcgagatgg taattgaagg
    21781 cgaaaaccta gatatgagca agttaagtat agacgaaaat ataattagca tagatggaac
    21841 tataaattct atggtatatg caaagcctaa aaagccacaa gagagtttct tgaaaaaggt
    21901 atttagatag atgatacctt ttactcaaga tgtaagcatt ttttatgcta ctatttatgg
    21961 tggaatactt ataggggtgt tgtttgattt ttatagaggg ctaagaggaa attttaaatt
    22021 catcaattat tttgcaataa tatttgatgt attgttttgg tttctagcta cagttataat
    22081 attcgtaaca ataaacctta cagaattttt tgatttaaga tattatcatt ttgtggcttt
    22141 gtttattggt tttattttat actataacac cataagtaaa attgttttat caattatcaa
    22201 taaaataatt cgttttgtga gaaattcttt taaaaaagtt acacattaca tagtcagttt
    22261 tttaaacaat ttgtattacg ttataatcta ttcactacac ctactatttg atattatatt
    22321 ttatatacca aacatattta tagcaacaag aaaaagtata aaaagaaaat caaataaaaa
    22381 atcaaagaac aagaaaaaat caaaacctaa gaagaaaaaa aataaaacta aaaaaagggt
    22441 gtaggaaaaa tgaacttaag aaaaaaattt tcaggacaga ttattgttat cagtttattt
    22501 ttaggaatat caatttttag tatgatgaca ggatttgtat ttgagtatac aaaagctaag
    22561 gaatataaaa aagaaatagc aagtctaaat aaacaattaa aaaagactga aatacaaata
    22621 aacgccttaa agaaagatga aaaatcatat gaaggagact tagaggatat agcaagaaaa
    22681 agattaaata tggtaaaacc aaatgaaaca gtttatgtag atataaatcg ttgattaaaa
    22741 cttgctaata agtttacaga tgtattgatt ttaaaattta agatttaata aaaaagtcat
    22801 tcagttaagg atggcttttt gtttttattg aggaggagat tttataatga aagtaggagc
    22861 tatagatatt gggacaaact caatgagatt attgatagca gattatagag atggaaattt
    22921 cataaacaga aaaaaatttg ttaatacaac tagaattggt tatggtgtgg atagtaaagg
    22981 atatataaag gaagatgcta ttaaaagaaa tataaaagca ctaaaagaat tttctaataa
    23041 gtgtaatgat gaaaagtgtg aaaaaattta ttgtatgggg acatctgctc ttagagacag
    23101 taaaaatgga gattattttg ttaaactagc taaagaagaa actggaatag atgttgatat
    23161 agttacagga atagaagaat caaatttggg atttaagggc gttttagaag gaatacaaga
    23221 tgatgatact attattgtta tagatattgg aggaggttca acagaattta ttgtaggtga
    23281 ctcagaagga attaaattta ataaaagtga aaatgtaggt gctcttagga tgacagagag
    23341 atttttgaag catgatatta ttgaagatga agagcttgaa gctatgagtt catttatata
    23401 tgatgagatt gacaaaacgt taaatcatat aaaatacaga ggattaaaaa ctcttatagg
    23461 tataggtggt acgattacat cattatcagc aatgaatcaa gaattagagg tttactctat
    23521 ggaaaaaata cataatagta aaatacacaa aaaagaaata gaagaaattc tacaaaaatt
    23581 aataaagatg acattaagtg ataaaaagaa cttaaaaggg ctacaagaaa aacgtgcgga
    23641 tattataact gctggtgtaa aaatattgaa tataataatg gaaaaattag aaatagaaga
    23701 aataaccgta agtgaatacg ataatttgga aggcttaata tgtcaaaagt caaaaaaaat
    23761 gtattaatat tttttgattt ttaaaaccga ttatgacaaa aaaaattact cctctttgtt
    23821 acaattctta atataaacaa aagaggaggt ttttttatgc aaagaagtgt agcagttgtg
    23881 aagaataaga ctgtaaaacc aaatgtcata aaattaataa gcggatatat tgattttact
    23941 acggcggcat tcgctgtgat gggattttta cttagtaggt ctattatagt agactctgta
    24001 gctcccttag gaattgcctt ttttatatgt gcatcaaaga ttgataagta caaaatacct
    24061 gtattcatat caacattatt aggaatattg ttatcatcta atgattttgg aaatgtagca
    24121 aaatatgctg tgtgccttac aatatttatg gctatgaata aaaaattaaa attatcagat
    24181 tcaattgcta aggttgcatt tataggtgca ataatactac ttccaatctc cataggacaa
    24241 gctttctttt caaataggta tgtgtatggt cttcttattg caggagtaga agcaataata
    24301 atgtttatat ctatatatgt gttttcttat ggaataggtt taatggccaa tgtcaacaat
    24361 aggatgtctg tacggacaga agaagctata tcaataagtt tactgctaac ctttagcata
    24421 atgggaatag gagatatatc cctgtttgga atatcaataa ggaccatttt atcgactgta
    24481 ttgatattac tgacctcgat agtaggtgga gctaccatgg gagcatctag tggtgtaatt
    24541 attggtgtag catccatttt aaataatatg acatcagcta tttatatggg aatatattct
    24601 ttttcagggt taataagtgg agcatttaat aagataaata aatatttctg tatactagga
    24661 tatattctaa gttggacaat aatataccta tatacttctg gtattacttc taatatgatg
    24721 caattaagag atatactatt aggttgtttg atagtattgg tattgccaga gagattgttt
    24781 aataagattg aaaaattaat aaaaagtaat gtagcctcaa atgaaatcgt atacgattat
    24841 attatgagaa gcaagaattt gactaatagt cgattgaaca gtatttataa aacttatgac
    24901 gaccttgcag atacgtttga taagataaga gaaaaagaca aagtattaga ccagagagat
    24961 atagctaacg taatagatat gattcataat gatgaatgta aaagttgtag tatgagaaga
    25021 atgtgctggg aatcaagatt taatcataca tacactatga tatataacat tcttgaaaaa
    25081 attgaggaga aaggggagtt aagtctaaac gatataccta aaaattttag aaaggaatgt
    25141 atgaaaccag agtcaatagt gaaaatatca aatcactatt acaaaatgtt tgtattagat
    25201 tatgattgga gtgtgaagtt ctcagaaagt agaaagttaa tagcaaatca gataagaagt
    25261 atttcaaaat caattaaatc gttatctcaa gatttagaag gtgacataat gctagatata
    25321 gaaaaggaaa aaaatatata tgaacagcta gaaagatatg atataacagt tgataaagta
    25381 agttacttaa caaaaagtaa cagtgagttt gaaatatcaa tagaaaagaa aacttgccat
    25441 gatggatgta tgtgtgaaga taaaatcgta aatataattt cagacttagt aggagaaaat
    25501 atgtctgtta gaaaaatagg gtgtcactgt ttaggaggta aatgtaaggc aacattcgtt
    25561 aaatctcaaa aatataaggc tgttacagaa gtttcagcaa tgtcaaggga tggacatatt
    25621 ctttgtggag acaattacac atacatggag ataaatgatg gtaaatatat gatggctata
    25681 agtgatggta tgggaaaagg taaaaaagca tatgaagaat cttctgccac tattgatata
    25741 ctggaaaaaa tgatagatgc aaaaattaaa gacgaaatag ttattgatac tataaataat
    25801 atgttgctat taaagtcttc agaagagatg ttttcaactt tggatttggg aattttagat
    25861 ttaaaacgag gttgtttaga aactataaag atgggagcgt gttctactta tataaaaaga
    25921 gaggatggag aagttgactt aatatcatca tcatcattac ctgtaggtat attatcagat
    25981 gtaaaaattg atagaaaaaa tgtaaaagtt aaggaaggag actatgtaat aatggtttct
    26041 gatggaattg tagatgcagg aagaaataac aatttaggag ataattggtt aatatacttc
    26101 ttaaagaata tagagactac aaatccaaaa gaaatttcaa atttaatatt ggatagagca
    26161 ttagagttac aagcgttaca aatcgaagat gacatgactg ttttagttac gaaaatatgt
    26221 acaaaataag gtttgcaaaa ttatgtgtat aagatgtgga taacttgtgt ataatatgtt
    26281 aatagacttt tgaaaaaagt atagttatta acaaaaataa catataaatc aacaaaattt
    26341 gttgataagt atggcagaaa tgttaataaa atgtaaaaaa agaaaaaaca aaagctatta
    26401 tcaaaagaga ttcatgttga aataactaaa tttctatgta aagaaaacct gtgaatatgt
    26461 tgattattat gtggataact attaattcga tatttattta tatagtatga ataattgaat
    26521 aatatatcta aaaaaagcca tcctattatt aagtaaactt attgaaagga tggcttttta
    26581 tgaatagaaa attgtgcaaa gcaataatac ctttatttat ttctattttt tgcatgttaa
    26641 taataagtat aaaagctgac caaggatatc aaaagaatgg tgacagagca attactgtca
    26701 tgaataacaa tgatactgaa gatgatgctg aaaaaaaaca agaaatagac tatgaaaaaa
    26761 catgttggaa cttgcaatct ttgtttaaaa gtgatgaaca gtggaaaaaa gaacttaaga
    26821 gttttgataa agatactaaa gagttgaaaa attacatagg caaagtaaca aaatcgccaa
    26881 cacatttgtc ttttgcctta aacattaagg aaaaacttga tataagacta aataaacttt
    26941 ctgcctatgt aaaattaaag caagatacta ataaaaattc atataagtac ttagatatga
    27001 gtgatagcat gagtaaatcc tatgggaatt atatggggat ttgctcagat ttagaattag
    27061 aaatactaaa attatcaaac aaagattaca agaaaattat ttcaaataaa aatataaata
    27121 gaaaatatgg tgcatattta agagatataa gaagaaataa agtacattat ttggatgata
    27181 aatcagaaga tattctaagc aaagtatctt caataagtag tcttccaagc caagtatatg
    27241 aattatttag aaatatggat agaaaaacta atttgacacc agctcaatat gcaagtgaat
    27301 tagagtcatc agatagagaa aatagaaaaa aagcttatca agatgaactt ataacttaca
    27361 atgataatat aaatacaatt gcaggtttaa ttactggtca agttaaaaag aatatttttt
    27421 attctcagga aagaggttat gagtcttctt tagaaatgta cttagaatct gataatgtag
    27481 atgaaaaagt atacaataat ttgataagga ctgtaaatgg taatatggac agtctacaca
    27541 aatatataga tttaagaaaa aaagtattaa aattagataa ggttcattca tatgatatgt
    27601 ttgtgcctat ggttaaggct gttgataata atattaatta tgaaaaggca caaagtatag
    27661 tatattcagc gttgtcacct ttaggaaaag aatacaatga tgtagtatat aaagctttca
    27721 atgaaaaatg gatagatgtt tattcacatg aaaataaagt aagtggtgga tactgcttat
    27781 ctgtttatga aaatcatcca tatatacttt taaattataa taattcatta ggctcagttt
    27841 ctactttatc acatgagctt ggtcatgctg tatacgaata cttaagctct aagaatcaga
    27901 attattttaa ttcaaatcct tctatattta ctcacgaagt tgcatctaca acgaatgaag
    27961 cattacttta cgagatgctt ataaaagagg ctaaaggcaa tgatgaaaaa gcttattata
    28021 taacacagta cttagacttg ataaaagata ctctttatac acaaaccatg tatgctgaat
    28081 ttgaaaaaac tatacatgag ttagtagaaa aaggaaaaag tgtaaacgca ttagtattaa
    28141 atgatgtttg gggacaactt cttaaaaaat attatggaca aagctatgaa ttagaccaat
    28201 tatctaaggt cggatgggct agaatcccac atttttataa tagtttctat gtctataaat
    28261 atgctacagg ttgtagtgca ggagtaagtt ttgcccaaga catactaaaa aatggttccg
    28321 ataactatat gagctttctc aaaaagggaa gttcagatta tccaataaac ttgttaaaat
    28381 caagtgggat aaatttaact aatacaaagc caatagaaaa tacaatcaaa aaatttgaca
    28441 gtttagtagc agaacttgaa aagctattat aaacattatt ataatccaca tggtaaatat
    28501 atcgaattaa tgataaaata tactaaaagt ttaaagtcct atcaagatag gactttttat
    28561 ttattaatta gtgggagatg attctatgca agttaaagtt aaaaaggctg taataccagc
    28621 agcaggttta ggaactagat ttttgccagc tacaaaggca caaccaaaag agatgttgcc
    28681 aatagttgat aagccaactc tacaatacat aatagaagaa gctgtggcct cagggataga
    28741 ggaaatacta ataataacag gtagaaataa aaagtctatt gaagaccact ttgataaatc
    28801 tgtagaattg gaacttgatt tagagaaaaa aggtaaaaaa gaattgttag agatagtaca
    28861 aaacatatct aacatgatta acatacatta tataagacaa aaagagccaa aaggattagg
    28921 agatgcaatt tattgtgcga gacattttat aggtgatgag ccatttgcag taatgttagg
    28981 tgatgatatt gtagataatg atgtaccttg cctaaaacaa ctaacagatg catatgagga
    29041 gtatagaact acgatattgg gtgttcaaaa ggtaaatcaa gaagatacta ataaatatgg
    29101 cataatagaa gcaaaaaata tagaaggtag agtttataag gtaaaagaca tggtggaaaa
    29161 gccagagtca ggaaaagctc cttcaaatat tgctatttta ggtagatata taataactcc
    29221 tgaaatcttt gatattctta aggatttacc accaggtaaa ggtggagagg tacaacttac
    29281 agatgcttta aaaatattat ctaagaaaga agctatgtat gcatacaatt ttgaaggcaa
    29341 gagatatgat gttggtgaca agctaggatt tttagaagcc actgttgact ttgctctaaa
    29401 gaaagaagac cttaaggaag attttataaa atatttgaaa catgtatgta gtgaatttga
    29461 taaaaataac aatgtgttat acaataatac agatgaaaaa atagatttaa ttgaagtcaa
    29521 tgaaatcaga gaacttgaaa ttcaaaaata attaatgaaa ggtgatgtat ggtggcaaaa
    29581 tcaagtcaga aaagaattaa tagaataata gctgttggga ttgtgggaat ccttgtatta
    29641 actagtataa cagctgtttt gagtatgatt gcaactatgt aataaggtaa ataacagaag
    29701 ttgatataca taaatataaa aaccatatat aacttgatgt agttaaaaaa ttacaatatt
    29761 ttagttatat atggttttta taatttataa atgtaaaaaa aattttttat aatagttgca
    29821 tattttaaaa gaaaatatta ttataagata taaatatagt gataaattta taaaaacgtg
    29881 ctaagattgg acttgaaata cagtaatata gtaggtaata atgttttaaa ttttatttat
    29941 tgaccataat atataaataa tgatattatt agacttaact gtttaaataa aacttttctt
    30001 tttcatgcct tcaattttca aaataagtat gtatataaat gaatatatag atgaggagga
    30061 tttttttgat tttagatgat aaagtattag aaggattagg tagtaagacc ttagttgagc
    30121 tacgagagat agctaaagaa ttaaaaataa aatcaattac tacatataag aaaaatgagt
    30181 taattgagat tataaattcc aaaagtaaaa atgaggaaaa aataaacgaa gagactaaag
    30241 aaaaaaacga agatattaaa gaagacataa aaaatagaaa agaaaatata gagcgtgaaa
    30301 gtatagaaaa agaagttgaa tataagagcc ctaccaaatc atacaacaag aaagaaatag
    30361 atacccaaaa tatttcccaa aaccaagaaa atagaaatca aagaaatgat tataataaga
    30421 caacaagtgt aaatgattgt aataagccat caaatatatc taatagaatg gtcagaaata
    30481 ataataaaaa ctattatatg cctaaacaag ttgatgaatc taagatagta gatgaattta
    30541 acacatcaaa agaggatgaa gtagtaggtg tacttgaaat tctgccagat ggatttggtt
    30601 ttttaagagg ttcaaattat ttgtctactg aaggagatgt ttatgtttca ccatctcaga
    30661 taagaagatt taatatgaaa acaggagata agattaaggg aataactaga catccaaaaa
    30721 gtggagagaa atttagagcg cttttatatg ttcaaaaaat aaatgatgaa aaccctgaca
    30781 cagctataca aagaaatgcg tttgaaacat taacaccaat atttccagaa gaacgactta
    30841 cactggaaac taatagaaat gaaatagcta caagaattat agatttaatt tcacctatag
    30901 gaaaaggaca aagagggttg atagtagcac ctccaaaagc cgggaaaaca gttcttttaa
    30961 aaagcgttgc aaatagtata gctaaaaatc atccaaatgt tgaacttata gtactcctta
    31021 tagatgaaag accagaggaa gttactgaca tgaaagagtc tattgaagga gatgttatat
    31081 attctacttt tgaccaagta tcaagtcatc atgttaaggt tgctgagatg gtattaaata
    31141 gagctcaaag attagttgaa catggcaaag atgtagtaat tttactagat agtataacta
    31201 gattggcaag agcatataac cttacaatat ctcctacagg tagaacttta tctggaggta
    31261 ttgaccctgg tgcactacat ggacctaaaa agttttttgg tgcagcaagg aatattagac
    31321 agggtggttc tttgacaata ttaggaacag cgttagttga aactggttct aggatggatg
    31381 atgtaatatt tgaggaattt aaaggtacag gaaatatgga attacattta gatagaaaat
    31441 tagcagaaaa aagaatattc cctgcaatag atatttacaa gtctggtaca agaagagatg
    31501 acttattgct tgatgatgaa gaaaagactg ctctttggag attaagaaga gaaatgagta
    31561 ataattcagt aatggaaatt acagataaag tcattgaact tataaagaga actaaagaca
    31621 ataaagagtt tgtaaaatca ataaaaaatt tgtaaaatca ataatacaaa tgtaaatgta
    31681 gattatataa agtaatagaa aaatcacttg aatggcataa taagttgtgc tataataatg
    31741 tagttgataa tgtaaaaaga aagttaatat aattaatttt tttaaataaa gagaagaggt
    31801 gactataatg caaaaggaaa tacaaccaaa atacaatcca gttgaagtgc gttgtgcttg
    31861 tgggaacaca tttgttgctg gttcaactaa ggacgaaata aaagttgaaa tatgttcaga
    31921 gtgccatcca ttctatacag gaaaacaaaa gaacatagaa aaaggtggaa gaatcgacaa
    31981 attcaagaaa agattcaaaa tggactaatt tttataaaag tggagttgga ggaagccaac
    32041 tcctatttta ttacaaaaaa acattcatat tctactttaa attgtctttt tgtattataa
    32101 taataaatat gtaaaattga gtattctaaa tacaataaaa tgaggtgagt ggatggcaaa
    32161 acaagctgta ggtgggcaag ctgttattga aggtgtaatg atgcagtcaa aagacaaaag
    32221 agcggtagct gttagaaaaa gtgacggaga aatagcacta aaagaagata gaataaaaag
    32281 ttgggtaaga gataagaata tagataagat accttttgtt agaggttctt ttgttatgat
    32341 agatacaatg attcaaggca taaaaagctt aaacttttct tcagagtttt ttatggaaga
    32401 agctgaagag gataaatttg atttatttat aaagaaaatt tttaaagata aggcaaatga
    32461 tataatcata atattttcat tggttatagc aatgttactt tctgctggat tattcatttt
    32521 tatacctaca ttggtaggag gcgctttttc taaagtaatg cctaatgatt ttatgcttaa
    32581 tttaatagaa ggaatcataa ggatagctat attatttgca tatatagtat tgatttcacg
    32641 aagtaaggat atagaaagag tctttcaata tcatggagca gaacataaat ctatatattg
    32701 ctatgaaaat gatttagaac ttacagtaga gaatgctaga aaatttaaaa gattacatcc
    32761 tagatgtggt acaaatttct tatttatagt tatggctgta tcaataatat tgtttgcatt
    32821 ttttggttgg ccaaatccaa tccttagaat atttatgaga attatatgtg tgcctatagt
    32881 agcaggttta tcttatgaag taattagagt tcttgggaaa tatgataatg gatttactaa
    32941 aattattgca tatccaggaa tgatgttgca atattttaca acaaaagagc ctgatgatga
    33001 gcaattagag gttgctttgg aagcattaaa ggcagtagta gattaaagaa aaggatattt
    33061 gttatgacaa taaaagatat aattattaaa tattcagata tattaaaaga tataagtgat
    33121 actccaagat tggatacaga gttattgtta caaaaaacat tgggtgttga taggttatat
    33181 attcacttaa atctaaataa agagctaact gaagagcaaa aaacagaatt tataggtttt
    33241 gcagaagaaa gattaaatgg aagaccaata gcatatatag ttgaaaatag agagtttatg
    33301 ggattagatt tttttgttaa ggaaggcgta ttgattccaa gaccagatac agaaacttta
    33361 gtagaagaga taattgaaat atgtagagaa aaaaaagatg taagtatact agatataggc
    33421 actggttctg gagctattac aataagttta gcgaagtaca ttgaaaattc taaaatcatg
    33481 tcttttgata tatcagagat agccttagaa atagctaaaa aaaatgctat tacaaatgaa
    33541 gttgatgaaa aaataaaata tattaattct gatttattta cagcaataag taatagtaac
    33601 ataaaattcg atatcatagt ttcaaatcca ccatatatta aaaaacaaga tatagaaact
    33661 ctgcacacac aggttaaaga ttatgagcca tataatgctc ttgaaggtgg agaagatgga
    33721 ttagactttt atagaagaat aacagaacaa ggtaagaaat atttaaataa gtttggaata
    33781 ttagcatatg aagttggtca taatcaagct gaagatgtaa taaatattat gaaaagtaat
    33841 ggatataaga aaatatatac aaagaaggat attcaaggca ttgatagagt tgttataggt
    33901 tacaatatat gatataatca acaattgata tgttacattt aatcgaggtg aaagtatgtt
    33961 aaaaaaatta gaggtattgg aagatacata taaagactta agtgaaaaaa taggtgatcc
    34021 ggatgtaata aatgatcaaa aagtttggca aaaatatata aaagaacatg ctgacttaga
    34081 acctattgtt atgaaataca gagagtataa aagtgtttta gatagtataa aagaatcaaa
    34141 agaaatttta caagaagagt cagatgagga attaagagaa ttagctaaaa tggagttagc
    34201 tgagatggaa gaaaaagtag cacctctgga ggaagaaata aaaatactat tattgcctaa
    34261 agaccctaat gatgataaga acgttatagt tgaaattaga ggtggagctg gaggagatga
    34321 agcagctcta tttgcgggtg atttatttag aatgtattca agatatgctg aaagaagaag
    34381 atggaaaata gaattattga gtgcaagtga tactggagtt ggtggatata aagaagtatc
    34441 ttttatgata aaaggtaaag gtgcatattc aagattaaag tatgaatctg gagttcatag
    34501 agttcaaaga ataccatcaa ctgaatctgg tggaagaata cacacatcaa cttctactgt
    34561 tgcagtatta ccagaagttg aagatgtaga ggttgaaata aatccaaatg atttaagaat
    34621 agacgttttc cgttcttcag gtaatggagg gcaaagtgtc aatactactg actctgctgt
    34681 tagggtaaca catataccta caggtgaagt agtatcttgt caggatggaa aatcacaatt
    34741 aaaaaacaaa gagcaagctt taaaaatatt aaaagctaga ctttatgata aagcattggc
    34801 agaacaacac aaagatatag cagcagaaag aaaaagccaa gtcggaacag gagacaggtc
    34861 agaaagaata agaacatata acttccctca aggtagaata agtgaccata gaataaattt
    34921 aactttatat aagctagatg cattcttaga tggagatata gatgaaatga tagatgcttt
    34981 aattactgtt gaccaaacag agaaaatgac tgcaatataa gtaatgtttt aaagaagctg
    35041 tttctttcta gaaattattt tctattttga aacagctatt ttgcatttta ggtcatgtta
    35101 tcgtattcta ctgtatataa ttgttatata caagtatagg agggttatat gatactaaaa
    35161 gtaacaataa ttggactttt ggcaggtgtt atagggacag gattaggagg agtaatatca
    35221 gctattttta aaagagaggt tgataaatat ctaagttttt ttatgggaat atctggtgga
    35281 ataatgttgg ctgtagtggt gtttgatttg atgaaagaat ctatggacaa aatggggata
    35341 atcaatacag ttatatttac gtttgttgga gctctcataa ctatgtacat aaaaacaaaa
    35401 ttagatatgt ctggaaatat ggcatccgga tatcttatat tcataagtat attactacat
    35461 aatttaccag agggactagc tattggttct tcttttatgt caactgaaag tttaggtatt
    35521 acacttgcta tagttatagg tttacataat ataccagaag ggttagctat ggcattaggt
    35581 ttagtctgta ataaaatgaa actaagtaaa gttattttat ttactgtaat agctggatta
    35641 cctatgggtc taggaagttt tttaggtgtt tattttggag gagtatttac ttctcttata
    35701 ggagtatttc ttgctacagc aggaggtact atgatgtatg ttgtattgga agaaattttt
    35761 ccgcattcaa aaagtgtgta ttgtataatt ggtttcttgt taggtacaat gattgtaaac
    35821 tatatataaa attaaaatga aagcaggcat acaaaagatg aaaactataa taagtaatat
    35881 tgatataaat aatattgatt atgaggaaat aaaaatacaa gcaaaacttc ttagagaagg
    35941 taaaacagtt atttttccaa ctgagacagt ttatgggcta ggtgcaaatg cattagatga
    36001 aaatgcagta aagaagattt atgaagcgaa aggaagacct agtgacaatc cattaatagt
    36061 ccatatatat gaaaaagaag aagtatatga tttagccaaa gatataagtg ataaagctaa
    36121 acttgttata gaaaagcttt ggccaggacc tataacaatt atattaaata aaaaagatat
    36181 aattccatac aaaactagtg gagggcttga tacagttgca ataaggatgc cttctaatat
    36241 tattgccagg gcaataataa aagaagctgg tatacctatt gcagctcctt ctgcaaatat
    36301 ctcaggtcga ccatctccaa caaaagcaaa acatgtatat gaagagatga atggaagagt
    36361 agatggaata gttttaggag gagacagtaa ttttggattg gagtcaactg tattagattt
    36421 aacagaagaa actcctatga tactgagacc aggaagtata actaaagaag ttttagagga
    36481 acttcttgga gaagtaaagc ttgacccatc attaagtaaa aaagaagata atcaaaaagc
    36541 taaagctcct ggaatgaaat ataaacatta ttctccaaat gcagatgtat atatcatatc
    36601 tggaaaaaga gaaaatgtag caataaagat taatgatatg ataaaatcta atagagaaaa
    36661 aggtctaaag acaggtgtaa tgtgtactag taaaaaccga gagttttatg atggagaagt
    36721 aatcgattta ggaaactctc ttgaagaagt tgcaagtaat ctgtttgatg ctcttataga
    36781 aatggacaaa aagagtgttg atgtcatata ttcagaagag ttccctaaga ctggagttgg
    36841 acaagcaatt atgaatagac tacttaagtc agcaggatac aaagtgataa aagcttaaaa
    36901 gggagttgta aaatatgaat atacttattg tgtgtacagg taatacttgt agaagcccta
    36961 tggctgaggc catattaaag aaagcaatta aagaatcggg aagaagtgtt gaagagtatt
    37021 ctatatcatc agcaggtata tctacagcaa atggaatgag tgcttctgaa aattctataa
    37081 aagcactaaa ggaaatcggt atagacttat caaatcatag aagtaaagta attaccaaaa
    37141 aattaattga tgaatcagat ataatactaa caatgactaa atctcataaa gaaattttat
    37201 tacaagcagt tccaaaatgc aaagaaaaag tgtatacttt taaagggttt gcaaataaaa
    37261 atgaggaaga tatatctgac ccatttggtg gaaatttgga tagttataga agcactatga
    37321 gagaaatagt gtattctgtc aacgaaatag taaaaaaaat atgaaacgga ggataaataa
    37381 atgaagatag gattaggctg tgaccatgga ggatataatt taaaaaaaga aataataagc
    37441 tatttagaag gtaaaggtat agaatgtgta gattatggaa ctaataatgc gacagattct
    37501 gttgattatc ctgtttatgg agaagtagtt gcaaattcgg ttataaataa agacgtagat
    37561 tatggaatat tatgttgtgg tacaggaata ggaatatctt tagctgctaa taaggttcct
    37621 gggataagat gtgcagttgt atcagatgta ttttctgcaa aaatgtcaaa agcacataat
    37681 aatgcaaata tgttatcatt aggagaaaga gttttaggaa aaggtctagc acttgagata
    37741 gttgaagcat ggattaatac agattttgaa ggtgatagac atgctagaag agtaaatatg
    37801 attaaatcaa tagaagaaaa acataacaaa taataaaaat tacagtttta tcaggaggaa
    37861 ttatcaaaat gagcaaagtg atagagacaa accatccatt aatacaacat aaattaactt
    37921 taatgagaga taaaaatact ggttctaagg attttagaga gcttttaact gaaattgcaa
    37981 tgttaatggg gtatgaaata acaaaagata taccattaaa agatgtagaa atagaaactc
    38041 ctattcaaaa aacatcatct aaagtagttg caggtaaaaa attagcaata attccaattt
    38101 taagagcagg tcttggtatg gtagatgggt tagtaagttt aatgccagca gcaaaagttg
    38161 gacatgtagg actatataga gacccagaaa cattaaaacc agttgaatat tactgtaagc
    38221 ttcctcaaga cataggagaa agagatataa tagtagttga ccctatgctt gcaacaggag
    38281 gttctgctgt agcagctata gatttactta agtctaaagg agctaaaagt ataaaattag
    38341 ctaacttagt agctgcacca gaaggtatag cagaagtaca aaaatatcat gatgatgttg
    38401 atatatatgt tgcatcagtt gacgaaagat taaatgaaca tggatatata attcctggtc
    38461 ttggtgatgc tggagataga ttgtttggaa ctaaataaaa ataaataact ataaatattt
    38521 attagggact ttcttatata gtgtatataa ttttatgtaa gaaggttcct ttttatatat
    38581 tttctcaaaa taggaattat tgaattgata aatttctcga attgagaata aaaagttttt
    38641 accaattaaa aaagattgct tattttacag ggttttatag aatagtagtc tattttaatg
    38701 tggcatgtta gttgcttacg attaagtgta tagatattaa gcgtattagt taaattaagt
    38761 gttgaatatt aataaatatt attgggagtg gtaatacatg aagaattgta tagtagtaag
    38821 aggagctgga gatttagcaa cagcagttat tcataaatta cacaacagtg gttttaaagt
    38881 agttgcgtta gaaatagaag aaccattagc tataagaagg caaatttctt tttgcgaggc
    38941 tgtttatgaa aaaaaagtaa ctgttgaaga tgtggaatgc atattttgca atactgaaaa
    39001 agaaatagaa gaagctttat ctcaacgtaa agtagccttg attatagacc caaaaggaga
    39061 aaaattatct aagttaaatc caatcgtttt ggttgatgga atacttgcta agaaaaattt
    39121 aggtacgact aaagatatgg caccacttac aatagcttta ggtcctgggt tttgtgcagg
    39181 cgaagatgta gatgttgtta tagagacaat gagaggtcat aatctaggaa gaacaataac
    39241 taatggtaga gcagaaaaaa atacaggagt accaggagca ataaaaggtt tttctaaaga
    39301 gagagtaatg cattcacagc atgatggtat aatggaaaat gtatgtaaga taggtgatat
    39361 agttgaaaag ggagacctac tttgctatat taataattat gaaaaaaaac atgaagttag
    39421 agcaacaata tctggagttc ttagaggttt attaaaagat ggagcaaggg tcaacaaaaa
    39481 tttaaaaata ttagacatag accctagaaa agaagaagtt aagaatgctt ttacaatatc
    39541 agataaagct agatgcatag ctggaggggt tttagaagcc atactaaaca atgggattta
    39601 tccccaaaat taatttatac tagataaagc tattaatatt ttattaagat gatataataa
    39661 atttaataga ttaaagagag catatgacgg gtatttaaaa tttaaatggt aaaaatgctc
    39721 tttttttatt ggacattcaa ttaacatgtg tatataatat aaaaataagt atttatttag
    39781 gttgaaatct aataaataca taataccagt acataagtaa agaggagaaa agtaaattat
    39841 gagaccatca tgggacgaat acttcatgga aattgcagaa gtagtgaaaa agcgttcaac
    39901 ttgtattaga agacaagtag gagctgttat tgtaagagat aaacaaatat taacaactgg
    39961 atataatggt tctccaagaa atcttgaaca ctgtgaaaat attggatgta agaggcaaga
    40021 gttaaatatt ccctcaggag aaagacatga attatgcaga gcattacatg ctgaacaaaa
    40081 tgcgataata caagctgctc ataatggcat tagtgtagat ggggcaactt tgtatgttac
    40141 tacaagacct tgtgttttgt gtgcaaaaat gtgcataaat gcaggtatat taaaaattgt
    40201 ctatgaaggt gattatccag atgatatgtc tacagaatta ttaaaagaag caggaataga
    40261 actcataaaa ttttagtaga caaaaattat atgacaataa gtgccaaatt gtgacactgg
    40321 aatacaatgg tattaaatga ttaaaagaaa gtatttgaaa aatcatcaga ttagtagtaa
    40381 gatatatttt ataattaaat aaatttatga gatagattaa ttacatgtta tttattttgt
    40441 aataatgagg gatactattt atgagtaaaa gaaatacaca tacagaaatt gcaaagttgt
    40501 ttagtttaat aagtcaaata ggattgatga ttgtaatatc aatattagga tgtactttct
    40561 taggaaaatt tttggatagt aaattgaata ctactccagt tctgacaata atatttttat
    40621 tgttgggtgt tggaggtgct tttagtggtg tttataaaac tctaatagta tatacacgaa
    40681 ggaagtgata tattggatat aaaattactt aaccaggtaa ggtcagtaac aaaaggtata
    40741 gtaatatttg atgtagtttt aattgtgtta atgttgataa catcaacatt aagtaaacca
    40801 gtattattag gacttatatt tggctctata atagctatgt taaattttag attattagcg
    40861 atatcactgg aaaagtcagt gacatttcct gctgggaagg cacaagctta tgcatcagca
    40921 caatatgcta ttagattatt tataatggca gttgttctat ttgcatcagc tacggtacca
    40981 catttaaata taatcggaac agcattggga ttattgagca caaagtttgt catattatca
    41041 aaaaacttta taaataaatt aaagaggaag gaggcataag tatgaatcaa gcgcattatt
    41101 atctcatagg aaactttaag attagtgaaa caatactagt aagttgggga ataatggcct
    41161 ttttagcgat agcatcttat tttttgacta gaaacttaaa aaaagttcca actagtaaag
    41221 ttcaaatatt cttagaattt gcagtaggtg gtcttgctaa aatggttaca gatgttatgg
    41281 ggaaaaatac agtaaaaaga atgccatata tgataccata cataggaagc ttgtttttat
    41341 ttttcgcatg ttcaaattta agcccattac ttggattaag gtcaccaaca accgacttag
    41401 acacaactgc tgcatgggca atgattacat tttttatgat ttattttgca ggagttaaat
    41461 ttaaggggtt atcatacttt aaagaattgg ctcaaccact tgccataatg ttaccaatca
    41521 atattatagg tgagttagct agaccaatat cattaagttt ccgtccattt gggaatattt
    41581 taggtggaac aataataatg ggtctgtttt atcaactttt aggttttata tctagtttga
    41641 taccaggatt atcaatacca attggacaga tattaatacc agtcccatta cacttgtact
    41701 ttgacttatt tgcaggattg ttacaagcat atatattcat aatgttaaca atggtattta
    41761 tatcaagtgc aggagagtta gattaagaaa tcaacttata ttaagataaa aataaaataa
    41821 tttaaaaaat taaagtttta aggaggaaat aattatggaa acagcaatag tagcggcagc
    41881 atcagcaata ggagcaggga tagcagttgc aacaggtata ggtgcaggta taggtcaagg
    41941 tatagcagca gctaaagctg ctgaagcagt aggaaaccaa ccagaagcta aaggtgatat
    42001 aacatcaact ttattactag gagttgctat agcagagtct tcagcaatat atggtttggt
    42061 tatatcaata atacttctat tcgtaaatcc attctttaaa tacttaggaa tgtagattta
    42121 gtccttaaat attttgagat aggtttatag gtaaatcatc tcaaacttat aatcatgaga
    42181 tgaactttat catctcgtga ttataagtag ttatgattta tggaaaggag gataattatg
    42241 gagaaagcac tagtaggtat aacgtgggaa tttgtattcc aaattgttaa tacttttata
    42301 atatttttac ttctgagaaa gcttttattc aagccagtat taaatataat tgaatcaaga
    42361 gaaaatgata ttaaaaatga tttggcagaa ggcgaaaaag caaaaaatga aggtttagct
    42421 ctgaaaaaag aatatgagtc aaaaataaat tttgccaaag atgaaggtca agaaatcata
    42481 aaacaagcaa ctataagagc agagcagaaa tcagatgata tagtaaatac tgcaaagaaa
    42541 gatgctcttg atataaaaga aaaagcaaac aaagatatag aacaagaaaa acaaaaggtt
    42601 ataaatgaaa tcaaaaatga tatatctaac atagcactac ttgcagcatc taaagttatt
    42661 gaaaaagatt tagataaatc taaacatgaa gaattgatag aaaactttat aaaagaggta
    42721 ggggaagcta aatgataaat gtaatagcaa atagatatgc cgaagcccta tttcaattag
    42781 gagaagaaga aaattcaact gatgttttat tcaaagaatt agaaaaagta gtagatatga
    42841 tgactaaagt tagtaaagat ttctacaaag ttttaaaatc accattagtt tcaaaatcag
    42901 aaaagaaaaa cttggtagaa attattttta gtaaagaagt aagttctaac ataaaaaact
    42961 ttttaaaagt tttagtagat aaagacagaa tatcatattt agaagatatc gaattagctt
    43021 ataaagaatt attaaataag aaaaataatg taattgctgg tgtagcaata agtgctattc
    43081 caatgagtga aacagatatt aaagaacttg aagtcaaact ttcaaataaa tacaacaaaa
    43141 atgttactat agaaaatgtt gttgacaaga ctatactagg gggagttcta gttagaattg
    43201 gaaatgaaca aatagatggc actgtgaaaa ctcgtcttga taagatgaaa gaaaagctat
    43261 ctgaagtaat atcttaagaa tggcggtgaa cccatgaact taaaacctga agaaataagt
    43321 tctataatta aacagcaaat aaaaaattat gagaataaag ttgagttgac agatacaggt
    43381 agtgttttaa cagttgggga tggtatagcg agtgtatatg gattagaaaa agctatgtct
    43441 ggtgagttgc tagagttccc aggtgaaata tatggtatgg cacttaactt ggaagaagaa
    43501 gtagttggag ctgttatcct tggagatgac agtgaaataa aagaaggcga tatagttaag
    43561 agaactggaa gaatagttga agttccagtt ggagaagctt taataggtag agttgtaaac
    43621 tctttaggtc agccaataga tggaaaagga cctatagcgt acacaaaaac tagaccagtt
    43681 gaatctgagg cacctggtat aatagataga aggtctgttt atgaaccatt acaaacagga
    43741 atcaaatcaa tagattcaat gataccaatt ggtagaggtc aaagagaatt aataataggt
    43801 gacagacaaa ctggtaaaac atctatagtt attgacacta tactaaatca aaaaggaaaa
    43861 gatgttatct gtatatatgt agcaattggg caaaaacgtt ctactatagc tcaattagtt
    43921 agttctttag aaaaaggtgg ggctctagat tatacaatag tagtttcagc aacagcatct
    43981 gaatcagcac cacttcaata tatagcacca tatgctggag cagctatggg tgaagaattt
    44041 atgtacaatg gaaaacatgt acttatagta tatgatgatt taagtaaaca agccgttgca
    44101 tatagagaaa tgtcattgtt acttagaaga ccaccaggac gtgaggcata tcctggagat
    44161 gtattctatt tacattcaag attacttgaa agagcagcta agttatctga tgaattgggt
    44221 ggaggttcaa tgactgcctt gcctataata gagactcaag ctggtgacgt ttctgcatat
    44281 ataccaacaa atgttatatc tataacagat ggtcaaatat acttacaacc agagttattc
    44341 tattcaggtg taagaccagc agttgaccct ggtatatcag tatcaagggt tggtggttct
    44401 gcgcaaatta aagcaatgaa aaaagttgca ggaacattaa aacttgcata ttcacaatat
    44461 agagaacttg cagcattctc acaatttggt tctgacttag atgaagatac taaaaagaga
    44521 cttgctcaag gtgaaagaat cgttgaaata ttaaaacaag gtgagcatca accaataaaa
    44581 gttgaaaatc aagttatgat aatatatgct gttataaata atcatctaga ggatattccg
    44641 atagataata tagctagatt tgaatcagaa ttgtatgcat ttgtagataa taattatcca
    44701 gaaatatcaa gaaaaatatt aggtggagaa gacttcactc atgatttaac agatgcaata
    44761 aatgaattta aagaaaagtt tgttgttgaa gtataatcct ttttaaaaaa ggaggtaaat
    44821 taattggcag gagctggaat caaagcaatc aaaacacgta tgggaactgt tgaaagtaca
    44881 aagcaaataa ctaaagcgat ggaactagtt gcatcttcta agcttagaaa agcaaaagaa
    44941 aaagctgaag attcaagaga atattttaat actttataca atacagtaaa agatattgcc
    45001 caaacaacac gtggaattag aaatgaattt ttaaaagaaa gagaagtaaa aaataagtgc
    45061 tatgtagtag tagctggaga cagaggactt gcaggtgggt acaattccaa tgtattaaag
    45121 gagtctgtct ctcatatgaa aggcaaaaag gaaacagtta ttactgttgg aaagaaagcg
    45181 catgaatttt tttctaaaag aaactacaat atagtgaaag atataaatag tgttgaagct
    45241 tgtggatatg aagatgcgac agaaatttca caaactatta ttgacctgta taaaaaaggt
    45301 gaagtagatg aagtttatat tgcttataca aaatttaaat ctgctttatc acaagaagtt
    45361 gaaattttaa aattgcttcc attagtcttt gaaaaagata cagacgaaca aggaatggaa
    45421 gtaaaagaag agaaacaaaa tagatcaaaa gtacaatata taccatcagc agaagcagtt
    45481 ttaagttata ttgtacctca gtttatatca ggtacagtat atggtggaat tttggaatct
    45541 tttgcatcag agcaaggtgc tagaagaact gcgatggaat cagccacaga caatgcgaat
    45601 gaaatgctat ctaatttaga gttaagttat aacagagcaa gacagtcagc tgtaactcaa
    45661 gaaataacag agatagttgg tggagttgaa gctctaaaat agggaggtta ggaaatggca
    45721 aatgtaggta aagtagtaca gattgtagga gctgtactag atgttaagtt tgatagtgaa
    45781 caaagcctac ctaacttatt aaatgcgtta gtaataaagt taggagataa agaaatagtt
    45841 gcagaagttg ctcaacatat aggtgatgat actgtaagat gtatagctat gagtgcaaca
    45901 gatggacttg taagaggtat ggaagttgta gatacaggag gaccaataag tgttcctgtt
    45961 ggagatgaga ctttaggtag aatattcaat gttcttggaa aaccagttga tgggaaacca
    46021 gctccaaaat ctgcacctaa attacctata catagaccag caccggcgta tgatgaacta
    46081 gaaactacag cagagatact agaaactggt attaaggtag ttgacttact agctccatat
    46141 ctaaaaggtg gaaaaatagg tctatttgga ggagcaggag taggaaagac agttttaata
    46201 caagagctta taaacaatat tgctaagcaa catggtggta tttctgtatt ttcaggagta
    46261 ggagaaagaa caagagaagg taacgacctt tatggcgaaa tgagtgagtc tggagttata
    46321 aataaaacag ctctagtatt tggccaaatg aatgaaccac ctggagcgag aatgagagtt
    46381 gctttaactg gacttacaat ggcagaacat tttagagatg agcaaggaca agacgtttta
    46441 cttttcgttg ataatatatt ccgtttcaca caagctggtt cagaagtttc agcacttcta
    46501 ggacgtatgc catcagctgt tggttatcaa ccaacattag ctactgaaat gggtgcgctt
    46561 caagagagaa taacatcaac taagaaaggt tcaataacat ctgttcaagc agtatatgta
    46621 ccagcagatg acttaactga cccagcacca gctacaacat tctctcactt agatgcaaaa
    46681 acagttttat caagacaaat atcatcactg ggtatatatc cagcagttga ccctcttgaa
    46741 tctacatcaa ggattcttga ccctagtata gtaggaaaag aacattatga agtagctaga
    46801 ggtgtacaat caatacttca aagatataaa gaacttcaag atataattgc tatacttggt
    46861 atggatgaac tatctgatga agataagtta atagttgcac gtgctagaaa gatacaaaga
    46921 ttcttatcgc aatcatttac agttgctgag caatttacag gaaatccagg tcaatatgtt
    46981 cctgttaaag agactgtaag aggatttaaa gaaattcttg aagggaaaca tgatgactta
    47041 ccagagtcag cattcttatt tgtagggaca atagaagacg ctgttaaaaa agcgaaggga
    47101 agtatgtagt catggccagt gaattttcat tgaaagttgt aactccaaat aatacttttt
    47161 atgatggaga cgttgaaatg atagtagtta gaactacaaa aggagacaaa ggggtattga
    47221 aaggtcatat accatttgtt tcaggtatac ctgtaggaac tttaaagata aaaaaagagg
    47281 gaagttttaa agaggcgaaa atagcagaag gatttgttaa tgtaactcaa gataaaactg
    47341 tcatattgac agaatctgct gagtggattt agtaatttaa tttaaaatat aaaaagtcat
    47401 tttgaccaaa aaacttttgg gtaaaatggc ttttttatat tttactgtta atagaataac
    47461 gataaaattg taatctttta gtgataataa attcacaaaa tgtttaaatt tactgcaaaa
    47521 ttatgttgta attaatataa taaagaagat ttagatagct tttatgtaag tgaaaaagtc
    47581 ttcatataag aaatacatat aaaattttta taaaatttaa aaaatttgct tagttgtgta
//
LOCUS       DAFNNB010000011        47369 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0011, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000011 DAFNNB010000000
VERSION     DAFNNB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 47369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47369
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0011"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            135..515
                     /locus_tag="L3M60_000548"
     CDS             135..515
                     /locus_tag="L3M60_000548"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBN6225251.1"
                     /translation="MNIFDIGVDIIEIDRIRKAIEKNNRFLEKIFTDREIEYFNSKNF
                     KAESIAGNFAAKEAISKSIGTGIRVFNFKDIEVLRNEMGKPIVKTYNNLAKMCIDYNV
                     LEIKVSISHSKDYAIANAITIIKD"
     gene            530..991
                     /locus_tag="L3M60_000549"
     CDS             530..991
                     /locus_tag="L3M60_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBN6225252.1"
                     /translation="MMDKTAKEIMTTDVIVAKQDDSIADVANMLIAEKIGGLPVVDSE
                     NRVVGIISETDILKKEKYIEAPLYINLLQGLIFLDDLKKVEKDIKQVAAYKVGELMSK
                     DIIKVHEDDKFDDVANVMIKKSINRVPVVDDDNKLKGIICRYDIIKALYNE"
     gene            1012..1602
                     /locus_tag="L3M60_000550"
     CDS             1012..1602
                     /locus_tag="L3M60_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225253.1"
                     /translation="MRKKWTIVCIMFLALVIIVIGCQKRQSTKEEVYKDFQKQISDMN
                     YYSCKAEVEVVGNKSPHNYVLIHTYKKTDNYKLEVISPKHLKGKSIEYQGDKILVKNP
                     KISDIVELPNTGKNNQYLFIGDFIKNYLQNEEMKVKLSKGHLVLETFIPGDNKYFNKQ
                     VLYVNADTKNPEKMEVLDKEGVPRFTVKYKDFEYRN"
     gene            1617..2774
                     /gene="alr"
                     /locus_tag="L3M60_000551"
     CDS             1617..2774
                     /gene="alr"
                     /locus_tag="L3M60_000551"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBN6225254.1"
                     /translation="MQKITVPTWAEINLDNLRFNLNNIKNLLEEDIKVCGVIKADAYG
                     HGAVEVAKLLEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKIT
                     MTVYSLETAQKINEIAKSLGEKACVHVKIDSGMTRIGFQPNEESVQEIIELNKLEYID
                     LEGMFTHFATADEVSKEYTYKQANNYKFMSDKLDEAGVKIAIKHVSNSAAIMDCPDLR
                     LNMVRAGIILYGHYPSDDVFKDRLELRPAMKLKSKIGHIKQVEPGVGISYGLKYTTTG
                     KETIVTIPIGYADGFTRIQKNPKVLIKGEVFDVVGRICMDQIMVRIDKDIDIKVGDEV
                     ILFGEGEVTAERIAKDLGTINYEVLCMISRRVDRVYMENNELVQINSYLLK"
     gene            2807..3094
                     /locus_tag="L3M60_000552"
     CDS             2807..3094
                     /locus_tag="L3M60_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HBN6225255.1"
                     /translation="MYDKSKEKIEVTLPDSLISEVDSIVQMENSNREDFAKAAFQFYI
                     TQKKKIHIKESMKTGYREMGQINLSLAELGMTVEEVSSDDKNEGKIAQGEY"
     gene            3063..3446
                     /locus_tag="L3M60_000553"
     CDS             3063..3446
                     /locus_tag="L3M60_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBN6225256.1"
                     /translation="MKGRLPKVSTNCEIKRGDLYYADLSPVVGSEQGGVRPVLIIQND
                     VGNKYSPTVIVSAITSQINKAKLPTHVEISSNEYGLNKDSVILLEQIRTIDKKRLREK
                     IGCLDENMMIKVDDGLQISLGLFTF"
     gene            3617..4444
                     /locus_tag="L3M60_000554"
     CDS             3617..4444
                     /locus_tag="L3M60_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBN6225257.1"
                     /translation="MRDHKGLNEIARIIRRDIVSMIHRAKSGHPGGSLSVVEILTALY
                     FDEMNVDSSNPKMEDRDRFVLSKGHAAPALYATLAEKGYFDKEELNGLRKIGRMLQGH
                     PDMKGTPGVEISTGSLGQGFSVACGMAMASKLDNAPWNVYTLLGDGEVQEGIVWEAAM
                     SAAHYKLDNLIAFLDNNGLQIDGDIESVMSLGSIVDKFKAFGWNVIEIDGHDFDQIFA
                     ALDIAKSTVKKPTMIVAKTIKGKGISFMENQAGWHGTAPSDEELEKALLELGGADNE"
     gene            4437..5378
                     /locus_tag="L3M60_000555"
     CDS             4437..5378
                     /locus_tag="L3M60_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBN6225258.1"
                     /translation="MSKMATREAYGKALVKLGQINDNVVVLDADLSKSTKTHDFYKAF
                     PDRFFNMGIAEQNLIGAACGLSTAGKIPFASTFAMFATGRAFEIIRNSVCYPKLNVKI
                     CATHAGLTVGEDGASHESVEDIAIMRAIPNMTVLVPADGVETEKIIFEIAKYNGPVYV
                     RLGRSSVPVLFDEDYKFEIGKGTVLREGKDVSIIACGIMVNEALLAQEKLQEEGISAR
                     VINMSSIKPIDKDLILESAKETNVIVTVEEHSIIGGLGSAVSEVVGESCPTIVKKVGI
                     KDTFGESGTPNELLKKYELTCDDIVKTVKEAIIAKRM"
     gene            5800..6894
                     /locus_tag="L3M60_000556"
     CDS             5800..6894
                     /locus_tag="L3M60_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225259.1"
                     /translation="MSEKVNVKTVISFAGAYVATVIGSGFATGQEIMQFFSFYGFAGI
                     IGGIISMVLFSWMGASVMSKGKELQLKEPIKIYRVYCGKYLGIFFEWFGPLFLFGVFV
                     VMISGAGATLTEYYGLNPFVGRVGMAVVALLTVSLGLDRLSKILGGIGPIIIIFTLLV
                     GGISLAKNIGNIGEAAQVLSTVKIAKPVPNAYLSGVIYTTYNTIVVMAFLTGLGASAA
                     NKKEAICGGILGGVALMAAAIMMHLAILSDIGNLYSKAIPSLFLADKISPIIGVLFSI
                     ILILGIYTTAVPLLWSVTNRFVDDNHPKFKLITLIAAILGLIGGFLPFDKLVGILYPY
                     TGYMGVIILVCVLYRQITKTSGYQEGISDK"
     gene            7120..7536
                     /locus_tag="L3M60_000557"
     CDS             7120..7536
                     /locus_tag="L3M60_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225260.1"
                     /translation="MDVKFNIKGIIYGVIALVFVIGGFIFIPSMFVEKSKPIDYTVLQ
                     RNSIPEKILDVMDKYTNEERALAAKIDNKIYIIVTRGNNKYGIEMDKIESVVEEGKDV
                     LRVKIKYKDKEDSFPYIVVETNMSELPDRIELNATK"
     gene            complement(7718..8287)
                     /locus_tag="L3M60_000558"
     CDS             complement(7718..8287)
                     /locus_tag="L3M60_000558"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBN6225261.1"
                     /translation="MKKEFRKKVIESRKNKNANFVLSNSNLITEKLLQMEDIRNATNI
                     MLYLDFNNEVQTDNLIKKLLSLKKIVSSPITIKENRTLIPTQITDLKDGLRIGAYGIR
                     EPNEKSPKIDIKALDVVIVPAVAYDIHCYRLGYGGGFYDRFLENLRKDAITIGIAFDF
                     QIFNSIPKETHDAQLDYIVTETRIITPNN"
     gene            8441..9598
                     /locus_tag="L3M60_000559"
     CDS             8441..9598
                     /locus_tag="L3M60_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HBN6225262.1"
                     /translation="MYLVISKKKAIFLGVILIIITAMVTSAFQLTLGNKVVISKELYE
                     DYKKYDKLLGLESIIKQDFYKKVSDTDLVNGASKGLFLGTNDKYSGYYTKDEMENLIN
                     DSEGSYVGVGMYIGASKDGGLVVVPMKDSPAEKAGVKSGDKLVKVNGKSVSYKNSDEA
                     VRMMKGKKGKTVELTILREDKQLNFKVKTDQIVEKSIESKVIDNDLGYIEITQFISST
                     YTDFDKALKELKAKNIKGLVIDLRNNPGGMLDICKEVADELIGEGTIVYTKDNKGNTE
                     YLKSDKEKLGLPIVVLTNGESASAAEILTGAIVDNKEGISVGTTTFGKGLVQSVVRLK
                     DGTGYKLTTAQYFTPNGDYINEKGIKPTIEEKDENKQLDVATKWLREQIDK"
     gene            complement(9780..10934)
                     /gene="nagA"
                     /locus_tag="L3M60_000560"
     CDS             complement(9780..10934)
                     /gene="nagA"
                     /locus_tag="L3M60_000560"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBN6225263.1"
                     /translation="MKSFIYADKFFMKHGTKEKGYLSIINGKFGEFQQERPSTTDNVI
                     DFSGKYIAPGLVDTHIHGLLGADVMDNTFEAINTISKGLLKYGVTSFLPTTLTDSVDT
                     LSDSVENISNSFNKVEGAKIQGIYLEGPFFTEKYKGAQNEKYFQDPDINILKLWQKKS
                     KNLIRKIAIAPERKGSIELTKYATKNNISVSLGHSSATFEQVKQVVNSGAKVFVHTYN
                     GMSPLNHREPGMVGAAMVLKDTYAELICDGHHVSPIAIKIMMDAKSSNNIALITDCMR
                     AGAMEDGKYTLGKFNVNVKNSVARLSSGSLAGSVLTMDRAVRNMVDWNISTLEDAIKM
                     ATYVPALSCNIDNVCGSIYQGMDADFIVTDKGFNIHETYINGICKYKVNK"
     gene            complement(11017..11742)
                     /locus_tag="L3M60_000561"
     CDS             complement(11017..11742)
                     /locus_tag="L3M60_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6225264.1"
                     /translation="MIKRNDKRPIYDQLVEILRTKIENEMEPNDRMLSERKICDEYGV
                     SRTTVRLAMAELEHMGYIYKRHGKGTFVAALSQNSQNLMESYSFTDYMRTQGKKPSTK
                     VLSFEVVESTKYFSEKLGITPGEKIIKIMRLRLANDLPMMLERTYLPMKEFIGLTKEK
                     IEQKPLYEIFREDYGEVIKVADEEFSAGIISDKEATLLEVPGDSPCLKLLRTTYNDDN
                     IVIEFTLSVARSDKFVYRVRHVR"
     gene            12012..12941
                     /gene="pfkB"
                     /locus_tag="L3M60_000562"
     CDS             12012..12941
                     /gene="pfkB"
                     /locus_tag="L3M60_000562"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBN6225265.1"
                     /translation="MITVITFNPSIDRMYRVNNINIGEVQRVVSKNATAGGKGLNVAK
                     VCKILKENPLAIGFLGGFNGEFIKNELKKLEIQNKFTKIEQETRNCLNIIEDNKVSTE
                     FLEKGPIVEKSDLERFENDIEEVLKNTKILVASGSYCENMPIDYYEKIGNICRANNIK
                     FILDTSGEALKIALKSKPYLIKPNIDEIRHLLGINIESRDEIILSGKKLIEMGAENVC
                     ISLGKDGMVYLNKDSVYDVKVPVVKCINTVGSGDSTVAGFSVGILRGYKIENLLKFAN
                     ACGISNALNMETGFVDLREIDKYQDLVEVKKLS"
     gene            complement(13070..13987)
                     /locus_tag="L3M60_000563"
     CDS             complement(13070..13987)
                     /locus_tag="L3M60_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBN6225266.1"
                     /translation="MKNIKMGMIGLGSIAQKAYLPILTKSERFEFVGAFTPNKVKREK
                     ICSDYRIMPFDSIESLAKKCDCIFLHSSTETHYEIIKILLNLGVHVYVDKPLASTVNQ
                     GEELIELSTKKNLNLMVGFNRRFCPMYKEIKNNATEIVSINICKHGLNSLRNVRFDST
                     LIDDYIHVIDTALWLANEDVEISGEDLFLTDNKNLIFVSHKLKGKHFSINTSMHRDSG
                     TKLEQVEILSKGRIQRIKNLNVLEIEEGGNLILKQSGAWVNILKQKGFEDISNHFIDC
                     IENNIKPAISGEECVKAQRLLEKIINSVK"
     gene            14459..15628
                     /locus_tag="L3M60_000564"
     CDS             14459..15628
                     /locus_tag="L3M60_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBN6225267.1"
                     /translation="MKMFKLEEQKLKDLGAIITTNEIKQQPELWLETYEIYKSNKEEL
                     RRFIDKISNNHGQFRVIFTGAGTSAYVGNSILPYLKNNNDIRKYIFEAIPTTDIVSNP
                     YDYLKKDVPTLLISFARSGNSPESLAALNLGNKIVDNFYHLAITCNPQGELAKMTKND
                     ENNYLLLMPSKSNDEGFAMTGSFSCMMLSAMLIFDSLEDDVEKSYINAIIEMGRNVID
                     RKDEIHELINKDFDRVVYLGSGGLGGLTQEAQLKLLELTAGKIATVYDSPMGFRHGPK
                     SFIDENTLVFEFVSNCLYTRKYDLDVLEEIKRDKIAKFTCAVSVENENNFSGTKFEFK
                     EKYNKLPDVYLAMPYILFAQTIALFVSVKVGNKPDTPSATGTVNRVVKGVTIYEY"
     gene            15677..16528
                     /gene="gatY"
                     /locus_tag="L3M60_000565"
     CDS             15677..16528
                     /gene="gatY"
                     /locus_tag="L3M60_000565"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBN6225268.1"
                     /translation="MALVTTTSMLKKAQDGGYAVGAFNVENMEMVIAVIEAAEELNSP
                     VILQTTPSTVKYAGLDYYLANAKVAAERVKIPVAMHLDHGSSFDLAMKALRSGYTSIM
                     IDGSHESFENNISISKSVVKACSPSLIPVEAELGKVGGKEDDLDGGNSNDYTNPKEAK
                     EFVDRTGISSLAIAIGTAHGLYKGEPKIDLDRLSEIKNVVSIPLVLHGGSGIHDDIIK
                     EAIKRGIAKVNYATELRIAYSKGVKKVLNEDSEVIDPKKYGLAGLQSVKDFVKEKIKV
                     CGSVNKV"
     gene            16857..18758
                     /locus_tag="L3M60_000566"
     CDS             16857..18758
                     /locus_tag="L3M60_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBN6225269.1"
                     /translation="MRERIILEALKNSSGYLNIDYFADKLGVSTRTIRNEIKKIETIK
                     ECNGFKLEYKSKLGYILNIKDQDKFEHYLRNLPSDLVENPEQRLESIIVELLVNEGYK
                     TIEQLSKKFLVSSSQIKNDLKKIDEKIKDTELKLERKAHYGIKIEGSIKEIQSILVDS
                     YFKGNRNITKYRNKFIDNIKLDNIRSTIKNVLNEHYLEANLTELEEILAQIIILYIRV
                     STRVVRSPSDLELYTEDLIIDELLDKIFGDKKYSLTYDEKYYLKQLIKLRTKDKKATI
                     KNIDKNKLQDIMCEFFRDIDKKYNSNFLEDKEFLNLFYLHIACLIERIKKNHKITNPF
                     SVKISQQYPTVFNLSIQFSKIIENEYHIKISQDEIGFIATHIAVPFEKREEANFNKKY
                     KIAIICSSGGGSAFLINLRLKEIFPNAEIKNFSLLEEKAVIEFAPDLIFSITNLLFEI
                     NAPVILINEILDELDYLDIKESFRFVDHIGNVSSPKQYILGLFDKNHFRCVKEKTKYK
                     DILDSMSQCIVDEGACSPTYPKDVWERESYLSTIYTNGVAIPHPIEMTGNKNIISVAL
                     IQTDINYENRVPKIIFMISLIKGNLELHKQISKYLSKIMTDKDMVDMLNKSQSYEEFM
                     YKLKIYLGG"
     gene            18761..19804
                     /gene="ypdE"
                     /locus_tag="L3M60_000567"
     CDS             18761..19804
                     /gene="ypdE"
                     /locus_tag="L3M60_000567"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBN6225270.1"
                     /translation="MNIEFFKRLSEADGIASNEEEVRHVLLEELRAYSDKVICDGLGS
                     IIFSKIKDESAPNVMICAHMDEVGFMVRSIDKLGMIHLMTIGGVKPLAQFVQKVRITT
                     KEGKKIHAIINATYNNGKAENIYADIGAYTEEDVYNLGINVGDMVTYTTNFEEFTLPD
                     RLVGKAFDDRIGCFVMGEVLKELKKENLNCNIHFAATSSEEVGIRGAKTSTQLINPDI
                     VFVIDVACAKNEFVRDYTNQKQIDKGVMLMHRDRTLVPSRKMIDYVMKIASENNIPLQ
                     HDMFESGGTDGGEAHLVNEGKPCVVTCVPVRYGHCAFSIVSNKDLENIIRLYTQLILN
                     FDEEKYRHIRNFI"
     gene            19819..20124
                     /locus_tag="L3M60_000568"
     CDS             19819..20124
                     /locus_tag="L3M60_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBN6225271.1"
                     /translation="MSTEVQIMELISTAGESKSKAFEALKKVKSKDFKGAKLLIEEAR
                     KIDIEAHRIQTELITQDLNEDVESPAMSLLMVHAQDHYMTSQLAKDLIEALIYCLDK"
     gene            20221..20556
                     /locus_tag="L3M60_000569"
     CDS             20221..20556
                     /locus_tag="L3M60_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225272.1"
                     /translation="MKILLCCAGGFSTNMLMQNMKKVVKESAKLNEKDFEFTAIPADS
                     IESEVDDWDIVLIGPQIAHKIDFLKGILEPKNKPFAVIDKDVYGKMDGATVMKLALIT
                     YKKHQLAQG"
     gene            20588..21910
                     /locus_tag="L3M60_000570"
     CDS             20588..21910
                     /locus_tag="L3M60_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6225273.1"
                     /translation="MFSKFEAFMNRVFMPLAHKVDNQRHLGSIKAGMVAMTPFTILGS
                     IFAILPALPNMLGEDNPISKFILNNSAILDLPVSLSIGLIGLYACMCIAFNLGNYYKL
                     YIPGCVALSTFSFLFLVASFTPDGALEIGNLGAKGLFTGMIAGLGSVELYNFCNKKNI
                     TIKMPENVPDFVSRSFELIPTTIIVCGTFIVARFISLKIFGELPPQILTQFLSPLVGS
                     MDNPWSVLALNFAICTIFFFGIHSSVFSPITRPIMITFIAENIAAMQAGETIPHFFTA
                     GASSAFFGFTGCGISIGCVIACMLSKNASYKKIGRVSLFPALFGINEPILFGAPIILN
                     PIMFIPHVFGGAIIGTLPMFFMHWGWLAKPIFDPPYVGVFLEGFLTNGDWHSILANAL
                     QLVLTVLLYWPFFKIMERQQNNKKQKEETKNIFSKEEEDLLDDLGLDF"
     gene            21920..22990
                     /locus_tag="L3M60_000571"
     CDS             21920..22990
                     /locus_tag="L3M60_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBN6225274.1"
                     /translation="MNNTIERLVSLIDKRGVDALLIKAKNTKRYIGALSGSGVYVLVT
                     KDKNYQVLDGRYIDEADKKTTGFIKRIVSQGSYIPTIIELLKELNIKKLAIENQAISI
                     QEYLSLKNAGFKINLITNELWKLRAIKSKEEIELIKKACEITDEVFDEVISEIKKDMT
                     ELEVSALIQYHALKKGASGMSFETIVVSGERGAMPHGRPTNKKLKVNEAITIDFGVVY
                     QGYQSDMTRTISIGKPPKIIKEIYDVVLEAQLSAIESIKEGTRASDVDKVAREIIDKH
                     GFGEYFNHGLGHGIGLGDGEVPTLNPNSEDILVEGMVMSCEPGIYIPNVGGVRIEDDI
                     VIIDGKGVPLNKTSKEFIILGE"
     gene            22995..24182
                     /locus_tag="L3M60_000572"
     CDS             22995..24182
                     /locus_tag="L3M60_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBN6225275.1"
                     /translation="MKYNFNKVLNRKEGNCRKWSNHVIKEKFGLNEDAIPMDLADIDF
                     ECAPAIKEAILKRASIGDYSYTFIGDDFYDAIINWNKRRFDVYIEKDWIKLTFGTVST
                     LHYIVQAYTKEGEGVLINTPAYDPFAEAVLNNNRKLCCSSLKLDNNRYYLDLEDMENQ
                     MKYENIRLLIFCSPQNPSGRIWTKEELYQVSELCLKYNVILVSDEIHRDIIFKDYRFV
                     SLWNAHPEIYKNSIICVSPNKGFNLGGLKTSYVLIRDLKIRETLLERLKSNSITSPNV
                     FAIPAIVAAYNESEEWLDAMTSYVEENFEIVYDFFETHIPKANVMKSDSSFLAWIDVR
                     GVFRNEEESKEFFRHANLTMVVGSYFVKDGDGFIRINVGCPRETLNEALNRIKKTYIS
                     MYC"
     gene            complement(24379..25251)
                     /locus_tag="L3M60_000573"
     CDS             complement(24379..25251)
                     /locus_tag="L3M60_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HBN6225276.1"
                     /translation="MNILQNENLIIESSSFGAELTKIFSKKYDKNILWNGNKKYWGRQ
                     SPILFPIVGKLFDNETIIEENLYSMTQHGFARDMDFEIVDKGDTFVSYKLTDNESTLK
                     KYPYSFELIINYTLNDNSIEVEWIVKNTDSKDIFFSIGGHPAFNLPSCEQNDFSKHYL
                     EFKSRNDVEKINLSGSFTDDIISIGKLKNLQLNPELFKNDALIYTNIDEVSICNNNGS
                     KYVTISMKDFPLVGIWTPYYSETNSTAPFLCIEPWYGLADNINSNKIYKDKKFINKLS
                     KGKVFTASYSININ"
     gene            complement(25645..25836)
                     /locus_tag="L3M60_000574"
     CDS             complement(25645..25836)
                     /locus_tag="L3M60_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225277.1"
                     /translation="MDDFSKLKSLYEDGYRCIYHDCVDNNYTIYLKNFYTEGSETIEL
                     SSESDFSQFKDYIDGLRMS"
     gene            26056..27102
                     /gene="cobT"
                     /locus_tag="L3M60_000575"
     CDS             26056..27102
                     /gene="cobT"
                     /locus_tag="L3M60_000575"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBN6225278.1"
                     /translation="MSLLESISKNIYSLDSSSIEKTKQRLDRLIHPTGSLGKIEDICM
                     QLAGIFGNENFDTSKKVIIAFAGDHGVYEEGVAPDPQNITKLQFPNFSKGLCGVGVIS
                     KFVGADVVAVDVGINCDEKLDGVLDYKIRKGTSNMAKGPAMSKQEAIRCLEIGIEIAE
                     QCIEKDYKVIGIGEMGIANTTPSTAIISVISGCDPLEVTGIGAGLKKERLKHKAEVIR
                     KAIEINNPNPTDGVDILSKVGGFEIGSMAGVILGCSANRIPVVIDGFISYAAALIAYK
                     INPKTREYMIASHLSAESGTKRALDILKLDPLLNMDMRLGEGSGAALAFNIIEASNYT
                     YKNMATFDEIDMGR"
     gene            27134..27694
                     /gene="cobU"
                     /locus_tag="L3M60_000576"
     CDS             27134..27694
                     /gene="cobU"
                     /locus_tag="L3M60_000576"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBN6225279.1"
                     /translation="MSKIILVTGGARSGKSNFAEKLCLDRNNNTAYIATSIPFDDEMK
                     DRVKKHQENRPRNWNTYEIYEDIYSIIKEIYKEHETVILDCVTLLVNNLMFKHNLNIE
                     ESSQEEINKLEQYIKEQVVKLIEEIEKTNLYFVFVTNELGMGIVPGNKLSRIYADIAG
                     RINQYIASKSNEVYFVVSGIPMKIKE"
     gene            27704..28477
                     /gene="cobS"
                     /locus_tag="L3M60_000577"
     CDS             27704..28477
                     /gene="cobS"
                     /locus_tag="L3M60_000577"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBN6225280.1"
                     /translation="MKRFILILQFLTRIPIKLNVGFDDEFYKSIVYFPLVGFVIGILS
                     YLVGWISMLLFEPFIASIIITLAGVLITGGLHIDGLGDTFDAIYSYRNKEKMLEIMKD
                     SRLGTNSLLAIMFVLLLKIGFIYDIISNNSLWVIIFMPMIARLGVMLLTYKTVTPREK
                     GMGNLFIGKLTTSMLITAIIYTLLIVALITKFIFLLPNIVLIKVLGSTIVVFVFIILF
                     KKHIYKKIDGVTGDILGCGIELSEVVYLIYIYLLNFMFF"
     gene            28497..29111
                     /locus_tag="L3M60_000578"
     CDS             28497..29111
                     /locus_tag="L3M60_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBN6225281.1"
                     /translation="MIRLILIRHALTNDNKKGKLSGHINSCISEEGKLQINKITRYLS
                     NENIDKIYTTPSTRTKDTIEKISKLKLLEIEEKETLREISFGDFEGITFEEIKIKYPN
                     EFEKMIKEGNNYRYPNGESLIDSYKRVAKEIDNIILENDSNLGSKTILICSHAGTIRN
                     IITHLISGSYKYHWNFKIDNASITVLDVDSGFTVIDKMNFTDFI"
     regulatory      29178..29357
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            29487..30986
                     /locus_tag="L3M60_000579"
     CDS             29487..30986
                     /locus_tag="L3M60_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HBN6225282.1"
                     /translation="MLQGTASNVGKSVLVAGLCRIFKQDGYTVAPFKSQNMALNSFIT
                     KEGLEMGRAQVFQAEAAKIEPIADMNPVLLKPSGNNKSQVIVRGKVVGEMPSSKYHDY
                     KLELVDVLKETFNSLNSRYDIVVMEGAGSNAEINLKDRDISNMGMAEIADAPVIIVGD
                     IDRGGVFASLAGTMLLLNEDEKRRVKGVIINKFRGKKELLQDGVKMLEDIIKVPVIGV
                     VPYTDIKIEDEDSVTTRFKKKMNKSDIHIEIIRTPHMSNFTDFNIFETQEDVSIRYVD
                     YGESLGNPDILIIPGTKSTIDDLTYIRKSGLESQIKNLHSQGKLIFGICGGYQMLGKK
                     LKDPYHVESEIEEVDGIGLLDTETTFEKEKTTTQVEATIVECISEYMNNLKGKKIKGY
                     EIHMGITKRGNDVRSLDLITKKLDKEVNYTEGSVNKEGNVIGTYLHGIFDEISFTRAI
                     LNNIRKKKGLGELESKVNSFNEFKDKEYDKLADFLREHLDIEKIYEIMK"
     gene            31025..32401
                     /locus_tag="L3M60_000580"
     CDS             31025..32401
                     /locus_tag="L3M60_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HBN6225283.1"
                     /translation="MKKILIAGTSSGVGKTTISLGIMQALVKRNMKVQPYKVGPDYID
                     PSYHTFITGRHSRNLDSYMLDDEKIKYIVNKSSKDADISVIEGVMGLYDGFGIDLDNC
                     TSSHTSKVLKSPVILVINGKSMAASSAAMVLGYRELDKDVNIKGVIVNNVKTNSHYQI
                     IKSSIEKYCNVEVLGYFPPNNKFSLESRHLGLVPSVEMKSLREKFYTLADEIEHYIDI
                     DRIIEISESDEFESDFDFQTLIENNKIKEHVNNKSIAIAYDKAFNFYYRENIELLEEL
                     GLEINYFSPLEDESVPNADYIYIGGGFPEIFGKELEANESMRVSILEAHNNNIPIYAE
                     CGGLMYLGEKLLNQEEQEFNMVGIFSGISKMTSSLKRFGYCIGVAKENTLLSKKGESI
                     KGHEFHHSIFESDEKCVYSMRKERDGNIVEEWDGGYSKGNTLATYLHTHFYNNLNCIE
                     NFLNKGNE"
     gene            32402..33373
                     /locus_tag="L3M60_000581"
     CDS             32402..33373
                     /locus_tag="L3M60_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBN6225284.1"
                     /translation="MNILSIYIGYVTDLIIGDPYSFPHPVRFIGKLINFTQGIIRKVS
                     KNDRQLKLGGFILWFITVGITYLTTYAIVKLFSFNILLSIVVNSFIIYTTLATKCLKD
                     EALKIYNVLKTEDIEKSRIQLSYIVGRDTTNLSEPEIIRATVETVAENTVDGIIAPMF
                     YAFIGGAPLAMAYKAINTLDSTVGYKNKKYKDIGFASAKIDDIANYIPARISVILMTI
                     GSFFLSYDHRNCFKISIRDRKNHKSPNCAFSEGAVSGALGIQLGGTNVYFGEKVYKPT
                     IGDKLREIDKEDIIKTNKIMYASSFVSMLVFTLIYVLCRMILINLII"
     gene            33405..34469
                     /locus_tag="L3M60_000582"
     CDS             33405..34469
                     /locus_tag="L3M60_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6225285.1"
                     /translation="MKDLGHGANVDEMARLYGKDPKEIIDFSSNINPNVLPNLERYIL
                     KGLEECRNYPDINYTNLRKNISKYIDINPDFIIPGNGATEVIYLLMKSIKKRLAIINP
                     TFSEYRRSAKLNNLDIIDLELESNFKLNIDIIKENIERFDSLFICNPNNPSGNVQDLK
                     ELVNLLGKHNKVLIIDETFMEFVEDESKHSLVKYIESNKNIFIIKAVTKFFGMPGLRL
                     GYGLTSNAEIMNKIYEYKEPWTINSFADILSNFIFEDREYIKDSKEYYIKERSYMLQE
                     LRNIRNIKVYDTDANFILIRTYKKTAKELKKDLFKQGNILVRDASNFIGLDDSFIRVA
                     IKSHKDNKILIENMKNLLGD"
     gene            34471..35358
                     /locus_tag="L3M60_000583"
     CDS             34471..35358
                     /locus_tag="L3M60_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBN6225286.1"
                     /translation="MKSYGICPASCGEFVQGIIDDEEYLCSYAIDMYSKVYIEEKLVD
                     INLGRYKSRLAIEKVFEKFNLPKKDTKNISLNINSKIPVGKGMASSTADIGATIKATL
                     SLIDKDLSSEEISKLAAEIEPTDSIFIDKNSIFNPLNGTVIKYLGNLTNAKVIILEPN
                     KVLDTMKIRLRQDYSRLKTENKEVIKKSFALLEEGLKKNNLSLVGEACTLSSLANENI
                     DKKEYLNEIIKISKKYGAYGVNIAHSGTVVGILIDKFMNDKNMIDALCESNINSVYSK
                     IYTLNIINGGIKGEIEWNT"
     gene            35346..35978
                     /locus_tag="L3M60_000584"
     CDS             35346..35978
                     /locus_tag="L3M60_000584"
                     /EC_number="5.4.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003724727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-8 methylmutase"
                     /protein_id="HBN6225287.1"
                     /translation="MEYIKNPMKIEEKSFELIQEIIDEIRPDYKFKNNIEEKIIKRAI
                     HTTADFDYLDILKISEEAVASIIDALRNNASIYTDTNMALSGINKRKLESLGCKYKCL
                     VADDETVKLAKERGITRSMAAVEIAAKEEGRKIFVLGNAPTALYKVMEMKSEGRLDLD
                     AVIGVPVGFVGAEESKDEVEKTDIPYIISKGRKGGSNLAAAIVNAILYTM"
     gene            35987..37213
                     /locus_tag="L3M60_000585"
     CDS             35987..37213
                     /locus_tag="L3M60_000585"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HBN6225288.1"
                     /translation="MEEYVYIDGKKYRRGYTTGSCATGASKAAVYMLITKNRINTINI
                     DTPKGIPLSLKVDNINISDTFVECSIKKDGGDDIDATHTMDIYARAEIVSKNDKNKEY
                     LTLKDIDGLSNNSESQSELYKFIRVYGGIGIGVVTKKGLSVDVGKPAINPTPLKMINH
                     EIRNLIGDNFESILGNNKVLKITIFAPQGEAVAKKTFNPRLGIVGGISIIGTTGIVEP
                     MSDEGWKKSLSIELQMKKEQGLDKIILVPGNHGEQFIREKLNLDIKYVVRTSNFIGYM
                     IKEAQRIGYKKILMAGHIGKFIKISAGIFNTHSKVADARSEILVANLALMGATYEFLN
                     KINQCLTTEEAVELINNSEYRQVYNILSNKCRERVKQYLNEDSDDIDVEVIIFSMDKS
                     LLGKSDNTDDLVEVFI"
     gene            37210..37818
                     /locus_tag="L3M60_000586"
     CDS             37210..37818
                     /locus_tag="L3M60_000586"
                     /EC_number="2.1.1.289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-7 (C(5))-methyltransferase"
                     /protein_id="HBN6225289.1"
                     /translation="MINIIGLGPGNLDYITKKGENLISTSDVLIGGKRNLESIKNFEG
                     EKIVLDSNLREIIEYINNNKEKQISIIASGDPLIYGIGRYISKNIDNKMLNMVSGISS
                     LQYIFSKIYVDMNDVYITSSHGKVPDFDYILSHKKVCMVTDSKIGPKQISREVIDRNL
                     NKIIVVGENLSYDNEKITIAKPEEIIRMDNFDMNVVVILDEE"
     gene            37808..38383
                     /locus_tag="L3M60_000587"
     CDS             37808..38383
                     /locus_tag="L3M60_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating cobalt-precorrin-6B
                     (C(15))-methyltransferase"
                     /protein_id="HBN6225290.1"
                     /translation="MRNSEFVTGKVPITKEEVRAISISKLNLVNAKNFIDIGAGTGSV
                     SVEAAYNYPNLKVISIEKNDDAIELIEKNIRKFNLKNIEVIKGYAPINLGLNTKVESI
                     FLGGTGNNLEEIIEWSKKNLVVGGRLVANFIIIDTFNQTLKLLKKHGFKEIDVCVLNV
                     SKLEKLGKGEYFKPLNPIYIISCEKGENDDE"
     gene            38376..39128
                     /locus_tag="L3M60_000588"
     CDS             38376..39128
                     /locus_tag="L3M60_000588"
                     /EC_number="2.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-4 methyltransferase"
                     /protein_id="HBN6225291.1"
                     /translation="MNKVHFVGAGPGDKELITLKGYKLLSDADVVIYAGSLVNPELLE
                     YCKEDCQIHNSAHMDLQEIIDVMREGIENNKSVVRLQTGDFSIYGSIREQVEDLNKLN
                     IDYDCTPGVSSFLGAASSLGVEYTVPEISQSVIITRMEGRTPVPEKESIQSYAKHQTS
                     MVIFLSVQEIEKVVSRLLEGGYPKDTPIAVIYKATWADEKIVKGTLSDIAVKVKENNI
                     NKTALIMVGRFLGEEYNNSKLYDKDFKHEYRG"
     gene            39112..40233
                     /gene="cbiG"
                     /locus_tag="L3M60_000589"
     CDS             39112..40233
                     /gene="cbiG"
                     /locus_tag="L3M60_000589"
                     /EC_number="3.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HBN6225292.1"
                     /translation="MNIEGNIAIICITENGKKLAVKINSLVNNCVIYQIKNKKSNLNV
                     EETCVHTVQKKLSDFVGEIFNKFDYIVFIMATGIVVRSIAPYIISKFSDPAVLVMDEK
                     GENVISLLSGHIGGANSLTQYISSLIDANPVITTATDVNQKASLDMIAKKLNAYIDTF
                     RESVKEVNSMLVNNKCVGLYIDEGYQVDTRGFKLLESKDELRDSFSNIYIEKLVVVSN
                     KINCIEDIENLNLDNKFELYYSTEKIIKVIPKDIVIGIGCRRNTDSELMYNSLKEFLN
                     KQNIDIRAINTVGSIELKKDEKAIIELSKTLKVPFKTFSIDEISKVDNLFDKSDFVKK
                     NVGVYSVAEPVAYLLSKGNLLVEKHKYKGITFSVGRIKI"
     gene            40230..40955
                     /gene="cobJ"
                     /locus_tag="L3M60_000590"
     CDS             40230..40955
                     /gene="cobJ"
                     /locus_tag="L3M60_000590"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011701973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HBN6225293.1"
                     /translation="MIYVVGIGPGSKDTMTLEAIKAIEDSEAIVGYKTYIKLIEEFIQ
                     DKEIIQNGMRQEVDRCKQAVAEAKKGKKVAVVSSGDAGIYGMAGLILELISKEDEDIK
                     VKVIPGVTASIGAAAILGAPIMHDFCHISLSDLMTPWEVIEKRLRLAAEADFVICLYN
                     PRSKGRSEHLSKAFKIMGEFKDGDTPVGVVKDVGREKEEKFVCTFENMDFEKVDMTTM
                     VIIGNKSTYIDGELMITPRGYTV"
     gene            40952..41704
                     /gene="cobK"
                     /locus_tag="L3M60_000591"
     CDS             40952..41704
                     /gene="cobK"
                     /locus_tag="L3M60_000591"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HBN6225294.1"
                     /translation="MILILGGTSDSLSICDKINELKNQPYILSVTTDYGRELAQQHAN
                     NVILGKLGKEDMLKFIKDNNIVKIIDATHPYAVEVSKNAITCAKLSNIDYIRFERKSL
                     IDEIDYDNKFIVNTIEEACEIANKIGTNIFIGTGSKNLNRFIEEISDKNLVVRVLPTS
                     EVILSCEKLGLSADNIIAMKGPFSQYINEETYKHYNIDLVITKESGIAGGFLEKVNAC
                     KTFNIPVIIIKRESINYENVFNDINKIKDILV"
     gene            41723..43201
                     /locus_tag="L3M60_000592"
     CDS             41723..43201
                     /locus_tag="L3M60_000592"
                     /EC_number="2.1.1.151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-factor II C(20)-methyltransferase"
                     /protein_id="HBN6225295.1"
                     /translation="MKKALLVVSFGTSYHETREKTIDICEDKIKNSLKSYDFFRAYTS
                     NMIINKIKKRDGIEIDNPIQALDKIYEQGYDEVIIQTLHIICGEEFNKLKEQVDGYSS
                     KFKKIVLGRPLLTHIEDYQEAVEAIKHQIPHMESDEAVVFMGHGTLHESHSAYPALEY
                     MLRDNGVNAYVGTVEGYPEIEHVIRRLKEGNIKTVNLMPFMLVAGDHAINDMAGDEED
                     SWKTILEEDGFNVRVHLKGLGENSYIQDKFVRHALKCAENAKFYGIGAGPGDGSLLTI
                     KAVNTLKKLDILYTPESKKGGDSLALSIVSEYLPESVEIKSRHFPMSFDGNEKSLAWD
                     NVAQEIISDVNDGKNVGFVTLGDPMIYSTYVYIMKRILGEVEVETIPGISSFSNIASN
                     QNFPLVMDKEALVVIPCTMEDDKIEYALQNYNSIVLMKVYKNFKEIIKKLEKYDLIEH
                     AILVSNSSQESENVFTDLKEAHLEDKISYFSTILVNKDNKIK"
     gene            43231..44136
                     /gene="hemC"
                     /locus_tag="L3M60_000593"
     CDS             43231..44136
                     /gene="hemC"
                     /locus_tag="L3M60_000593"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBN6225296.1"
                     /translation="MNIVVGTRGSNLALIQTEWVINELKKKYPEISFEIKIIKTKGDL
                     IQNVSLDKIGDKGLFVKEIEQQLLYGKIDIAVHSMKDMPSYLADGLKFAHTPKREDPR
                     DVLILREGYKNLDDLPRGAVIGTGSKRRKFQLLKQRPDLNIVQVRGNVETRIRKIKDE
                     NMHGIVLAASGIIRANLQDKISSYLPVDVVIPAPAQGALAIEIRSNDSKIEGIVNSLK
                     DENTEIQILAERGFLDGVNGSCHIPMAAYCEIIQDKIHLTGLYGDSEGKKVVIKSIDG
                     DISSPRELGLKLAKLVLKEYENYEG"
     gene            44126..45625
                     /gene="cobA"
                     /locus_tag="L3M60_000594"
     CDS             44126..45625
                     /gene="cobA"
                     /locus_tag="L3M60_000594"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBN6225297.1"
                     /translation="MKGKVYLVGAGPGDYKLMTLKGLECIRKSDVIVYDRLANSNYLR
                     EAKPDCEFIYVGKASSNHILPQEDINRIIAEKAKEGKIVTRLKGGDPYVFGRGGEEAE
                     TLVDEGIEFEVVPGITSAIGGLCYAGIPITHRDHASSFHVITGHLKGDDSGELNWNAL
                     ANNKGTLVFLMGISNLNKISENLMKEGKDKNTPVALISWATRYNQRVVTSTLENVYET
                     AIKEEVKPPTLIVVGSVVELREKLNFFESKPLFGKSIAVTRSRNQNSVLVEKIMDLGG
                     NPIEIPTIKVEKIQNNIKLENEIKNINKYNYLILTSKNAVEIFFEKIYEMNFDLRILS
                     NLKVCAIGSATSNELKKRGIIADIVPKKFVAESLYEELMPILNNSDNILIPRAENARD
                     YLVDKLKAICEVTEVHIYRTVIDEDKKDEILDILNSNNIDYITFTSSSTVNNLVEIIG
                     KENIKILEKIKTLSIGPITTDTINSLGIKLYKESATSTIDSLVDLIIKN"
     gene            45642..46613
                     /gene="hemB"
                     /locus_tag="L3M60_000595"
     CDS             45642..46613
                     /gene="hemB"
                     /locus_tag="L3M60_000595"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBN6225298.1"
                     /translation="MLRRPRRLRASKAIRNLVRETKVNVEDLIYPLFVVEGENIKEEI
                     KSLPDVYHLSIDMLEEEIKEISELGIEHIMLFGVPDEKDECASQAFNDNGIVQKAIRK
                     VKEINADMNVITDVCMCQYTSHGHCGILTDTGYVDNDKSLEYLAKISVSHAKAGANMV
                     APSDMMDGRIQAIREALDENGLENVGIMAYSVKYASNYYGPFREAAHSAPSFGDRKTY
                     QMDPANSNEAIIETELDILEGADILMVKPALSYLDIIRRVKDNFDLPLAAYNVSGEYS
                     MLKFAVKEGLLNEDVILESITSIKRAGADIIITYFAKDIAKLLKNKF"
     gene            46756..47334
                     /locus_tag="L3M60_000596"
     CDS             46756..47334
                     /locus_tag="L3M60_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HBN6225299.1"
                     /translation="MLYPINLKLDDLDVVIIGGGEVAYRKCKNFLNFNKRVTIVSKQL
                     LNKFYDLKEYINIIEDDYKEVYIEKASIVIAATNNKVLNMEIGLYCRAEHKLVNVVDN
                     VEISNFTVPSYIKRGDLLISVSTGGKSPSLSSKIKKEIEEIYTEDYEEYLNVLGDIRK
                     KVIKKYEDKSKRKDVLNMLITLDLEELKKFDI"
BASE COUNT        18744 a         4997 c         8551 g        15077 t
ORIGIN      
        1 tttaaaagtt acagttttat tataatgatt attaaattaa gataagtaac ataaactaaa
       61 taataatatg tattatcgat agtaaattca ttattaatta ttgaaagaag tattgtaaat
      121 tttctggagg aacaatgaat atatttgata ttggcgtaga cataattgaa attgacagaa
      181 taagaaaagc tatagaaaag aataataggt ttttggagaa gatatttaca gatagagaaa
      241 tagagtattt taattctaag aattttaaag cagaaagcat agcaggtaat ttcgcagcta
      301 aggaagctat aagtaaatct ataggtacag gaattagagt gtttaacttt aaagatatag
      361 aagttctcag aaatgaaatg ggaaaaccaa tagtaaaaac ttataataat ctagcaaaaa
      421 tgtgcataga ttataatgtt ttagaaataa aagtttcaat atcacattca aaagattatg
      481 ctatagcaaa tgcgataacc ataattaagg actaaggagt gattacatta tgatggataa
      541 aacagcaaaa gaaataatga caacagatgt aatagtagca aaacaagatg atagtatagc
      601 agatgtagct aatatgctaa ttgcagaaaa aataggaggt ctgccagttg tagattcaga
      661 gaatagagtt gttggaataa tttctgaaac agatattctt aaaaaagaaa aatatataga
      721 agcacctctc tatataaatc tattacaagg attgattttc ttagatgact taaaaaaagt
      781 agaaaaagat ataaaacaag tagctgctta taaggttgga gaacttatgt ctaaagatat
      841 aataaaggtt catgaagatg ataagtttga tgatgtagct aatgtaatga ttaaaaaatc
      901 aataaacaga gttcctgtag tagatgatga caataaactt aaaggtataa tttgtagata
      961 tgatataata aaagctttat acaatgaata aactgaagta ggaggtgtga tgtgagaaaa
     1021 aagtggacca tagtatgtat tatgttcttg gcactggtta taattgtaat aggatgccaa
     1081 aaacgacagt ccacaaaaga agaagtatac aaagattttc aaaagcagat ttctgatatg
     1141 aattattaca gttgtaaagc tgaagtagaa gtagttggaa ataaaagccc acataattat
     1201 gttttaattc atacttataa aaaaactgat aactataaat tagaggtaat ttccccaaag
     1261 catttaaaag ggaaaagtat agaatatcaa ggtgataaaa tattagttaa aaatccaaag
     1321 attagtgata tagtcgagtt gcctaataca ggaaaaaata atcaatatct ttttatagga
     1381 gactttataa aaaactatct tcaaaacgag gagatgaaag taaagttatc caaaggacat
     1441 ttggttttag agacttttat tcctggggat aataaatatt ttaacaagca agtattatat
     1501 gtaaatgctg acacaaaaaa tcctgaaaaa atggaagtgt tagataaaga gggagtgcca
     1561 agatttacag taaaatacaa agattttgaa tacagaaact aagggggact aaagacatgc
     1621 aaaaaataac agtgcctaca tgggcagaga taaatctaga taacttaaga tttaacttaa
     1681 ataatattaa aaatttatta gaagaagata ttaaggtttg tggagtaata aaagctgatg
     1741 catatggaca tggtgcagta gaagttgcaa aattgctaga aaaagaaaaa gtagattacc
     1801 tagcagtagc aagaactgct gaaggaattg aacttagaca aaatggcata acacttccta
     1861 ttttgaactt gggatatact ccagacgaag ctttcgaaga ttctataaaa aataaaataa
     1921 ctatgacagt ttattcttta gaaacagcac aaaagataaa tgaaattgca aaatctttag
     1981 gagaaaaagc ctgtgttcat gttaagatag actcagggat gactagaata ggtttccaac
     2041 ctaatgagga gtcagtacag gaaataatag aattaaataa attagaatat atagatttag
     2101 aaggtatgtt tactcatttt gctacagctg atgaagtaag taaagagtac acttataaac
     2161 aagctaataa ttataaattt atgtctgata aattagatga ggctggtgta aaaatagcta
     2221 taaaacatgt atcaaacagt gcagctatta tggattgccc tgatttaaga ttaaatatgg
     2281 taagagcagg aataatattg tatggtcatt atccatctga tgatgtattt aaagatagat
     2341 tagaattaag accagctatg aaattaaaat caaaaatcgg acatataaaa caagttgaac
     2401 caggtgtagg aataagttat ggactaaaat acacaactac tggtaaagaa acaatagtta
     2461 caattccaat aggatacgca gatggattta ctagaatcca aaaaaatcca aaggttctta
     2521 ttaagggaga agtgtttgat gtagttggta gaatatgtat ggatcaaata atggttagaa
     2581 ttgacaaaga tatagacata aaagttggag atgaggttat actatttgga gaaggcgaag
     2641 ttacagctga gcgtatagct aaagacttag gaactataaa ctatgaagtg ttatgtatga
     2701 tatcaagaag agttgaccgt gtttatatgg aaaataatga gcttgtacaa ataaacagtt
     2761 atttgctaaa ataaaaccat gatattataa tctgggaggc gattttgtgt acgataagag
     2821 taaagaaaaa atagaagtta ctttaccaga ctctctgatt tctgaagttg atagtatagt
     2881 tcagatggag aacagtaaca gagaagattt tgcaaaagct gcttttcagt tctatataac
     2941 tcagaaaaag aaaattcata ttaaagaatc tatgaaaacg ggttatagag aaatgggtca
     3001 aattaatctg tcactagctg aattaggtat gacagttgaa gaagtatctt ctgatgataa
     3061 aaatgaaggg aagattgccc aaggtgagta ctaattgtga aataaagcgt ggtgatttat
     3121 actacgctga tttaagtcca gttgttggtt cagagcaggg aggcgttaga cctgttctga
     3181 taatacaaaa tgatgtaggt aataaatata gtcctacagt tatagtatca gctataacat
     3241 ctcagattaa taaagccaaa ttacctactc atgtagagat aagttccaat gaatatgggc
     3301 ttaataaaga ctcagtaatt cttttagagc aaattagaac tattgataaa aagagactga
     3361 gggaaaaaat tggttgtcta gatgaaaata tgatgataaa agtagatgat ggacttcaaa
     3421 taagtttggg attgtttact ttttaaagag ggatttgtct tttattagac aatccctttt
     3481 ttcttttaaa tatctcttag tgtttaattt taatgtacta tgttaataaa attaaaaata
     3541 acgcaaaaaa atagcataaa agtgatataa taatatggaa ttgatataaa attacgaact
     3601 caagggaggt aattgcatga gagaccataa aggattaaat gagattgcgc gtatcataag
     3661 aagagatata gtttcaatga tacatagagc caaatctgga catccaggag ggtcattatc
     3721 agtagttgaa atacttactg cgttatactt tgatgaaatg aatgtggatt cttcaaatcc
     3781 taaaatggag gatagagata gatttgtatt atcaaaagga catgctgcac cagcattata
     3841 tgctacattg gctgaaaaag gctattttga taaggaagaa ctgaatggat taaggaagat
     3901 aggaaggatg ttacaaggtc atccagacat gaaaggcacg ccaggtgtgg aaatatctac
     3961 tggttcttta ggtcaaggct tttcagttgc atgtggtatg gctatggcat caaagttgga
     4021 taatgctcca tggaatgtgt acactttgct tggtgatggt gaggttcaag aaggaattgt
     4081 ttgggaagct gctatgagtg ctgcacatta taaacttgat aatttaattg cttttttaga
     4141 taacaatgga cttcaaatag atggagatat agaaagtgta atgagtttag gttcaatagt
     4201 tgataaattt aaagcttttg gttggaatgt aattgagata gatggacatg attttgacca
     4261 aatatttgct gctcttgaca tagctaaatc aacagttaaa aaacctacaa tgatagttgc
     4321 aaaaactata aaaggcaaag gaatatcttt tatggaaaat caagcaggat ggcatgggac
     4381 agctccaagt gatgaggagc tagaaaaagc cttgcttgaa ttaggaggtg ctgacaatga
     4441 gtaaaatggc gacgagagaa gcatatggta aagcattagt aaaattaggt caaataaatg
     4501 acaatgttgt agtgttagat gcagatttat caaaatctac aaaaacacat gatttttaca
     4561 aggcatttcc agacagattt tttaatatgg gtatagctga acaaaattta ataggtgctg
     4621 cttgtggatt atcaactgct ggtaaaatac cttttgcaag tacttttgct atgtttgcta
     4681 caggaagagc ctttgagatt ataagaaatt cagtttgtta tccaaagcta aatgtaaaaa
     4741 tatgtgctac tcatgcaggt cttactgttg gagaggatgg agcatctcat gaaagtgtag
     4801 aagatattgc tataatgaga gctataccaa atatgacagt tcttgttcca gcggatggag
     4861 ttgaaacaga aaaaattatt tttgaaatag ctaagtataa tggtcctgtt tatgttaggt
     4921 taggaagaag ttcagtacca gttttatttg atgaagatta taaatttgaa ataggaaaag
     4981 gtacagtctt aagagaagga aaggatgtat caattatagc ttgtggaatt atggtaaatg
     5041 aagctctatt agcacaagaa aagttgcaag aagaaggtat aagtgctaga gttataaaca
     5101 tgtcctcgat aaaacctata gacaaagatt taatattaga atcagcaaaa gaaacgaatg
     5161 ttattgtaac agtagaagaa catagcataa ttggaggatt aggttctgct gtaagtgagg
     5221 tagtgggaga gtcttgtcca accattgtaa agaaagttgg aattaaagat acatttggag
     5281 aatctggtac tccaaatgaa ttgttaaaaa aatatgaatt aacttgtgat gacatagtaa
     5341 agactgttaa agaagctatt attgcaaaaa gaatgtaaga gatataaatt attcatgttt
     5401 gttaataata taatatattt aaaagagagt tataaagtgc tatattaatt tatatctctc
     5461 ttttttttat atgatatcaa tgtatcaaaa atgtaataaa atattcaaat ttttacaaaa
     5521 atagagaaaa tattcaaata tatattgact attcagaaaa atatgactaa tatataattt
     5581 atagagaaat taattttata tataatgact acttagataa aagaatctaa aaaattatga
     5641 aaggaggact agggtataaa attagattgt aagttttaag ttattatact aaaaagctat
     5701 aaaacagtta tcatgtattt tatgttaaag gaaattgtag gtagtttatc gaattgttta
     5761 agcagaagaa aaaatttcac atggggaggt aactgagata tgtcagaaaa agtaaatgtt
     5821 aaaacagtca tcagtttcgc aggtgcgtat gtagcaacag taatcggttc tggatttgca
     5881 acaggacagg aaataatgca attcttctct ttttatggtt ttgcaggtat tattggtggg
     5941 ataatttcaa tggtattatt ctcatggatg ggtgcttcag ttatgtcaaa aggtaaagag
     6001 cttcaactta aagaacctat caaaatttat cgtgtttatt gtggtaagta tttaggaata
     6061 ttctttgaat ggtttggacc tttattctta tttggtgttt tcgtagtaat gatttcagga
     6121 gcaggagcaa ctttaactga atactatgga ttaaatccat ttgtaggtag agtaggtatg
     6181 gcagtagtag cattattaac tgtttcatta ggtcttgata gattatcaaa gatactaggt
     6241 ggaatagggc ctataataat aatctttaca cttttagttg gtggtataag tttagctaaa
     6301 aacataggta atataggaga agctgcacaa gttcttagca ctgtaaagat agctaaacca
     6361 gttccaaatg cttatctatc tggtgtaata tatactactt ataacacaat tgttgttatg
     6421 gcattcttaa ctggattagg agcaagtgct gctaacaaaa aagaagctat ttgtggtggt
     6481 atattaggtg gagtagcact tatggctgca gctataatga tgcacttagc tattctttct
     6541 gatataggaa acttatattc aaaagcaata ccatcattat tcttagcaga taagatttca
     6601 ccaataattg gagttttatt ctcaataatt cttatattag gtatatatac aacagctgta
     6661 cctttattat ggtcagtaac taatagattt gtagatgata atcatcctaa gtttaaatta
     6721 ataactttaa tagcagctat attaggtctt ataggtggat tcctaccatt tgataagctg
     6781 gttggtattt tgtatccata tactggatat atgggcgtca taatactggt ttgtgttctt
     6841 tatagacaga ttacaaaaac atctggatat caagaaggaa tcagtgacaa ataaatattt
     6901 tttaatattg taaacccaag agctatatga aaacaataaa aatgttttca tatagctctt
     6961 tttatttttt agctcgttta ttatgaaata ggaaaaacat gttttttagt tacattacat
     7021 atatttataa ttatggataa atatatacta atcaataatt tagagtctaa gataagttta
     7081 taaatcatat atttagaata tatgggagga gtgataagat tggatgtaaa gtttaatatt
     7141 aaaggtataa tttatggagt tatagctctt gtattcgtta tagggggatt tatattcata
     7201 ccaagtatgt ttgtagagaa atctaaaccc atagattata cagtattaca aagaaattca
     7261 ataccagaaa agatactaga tgtgatggat aagtatacaa atgaagaaag agcattagcg
     7321 gctaaaatcg ataataaaat atacattata gtgactagag gaaataataa atatggtata
     7381 gaaatggaca aaatagaaag cgtggttgag gaaggaaagg atgttttgag agtcaagata
     7441 aagtataaag acaaggaaga ttcttttcca tacatagttg ttgaaacaaa tatgagtgaa
     7501 cttccagata gaatagaatt aaatgcgacg aaataagaag gtattttata attaaataac
     7561 tgcatattgt acaacaaagt catggttttt attaatgtaa gtaaattgtg tatggttctt
     7621 attgttgtaa aagccatgac ttaaaatgct tataaagtaa gatattaaca gtagaattta
     7681 ttactatatt aaataaggtc tggaagtact taatgttcta attatttgga gtgataattc
     7741 tagtttcagt aactatgtag tctaattgtg catcatgagt ttcttttgga atagaattga
     7801 atatctggaa gtcaaatgct atgcctatag ttatagcatc ttttcttaag ttttctaaaa
     7861 atctatcata aaatcctcca ccatatccta atctatagca atgtatatca tatgctacag
     7921 ctggtacaat tacaacatcc aaggctttaa tatcaatttt aggtgatttt tcgttaggtt
     7981 ctcttattcc atatgctccg attctcaatc catcttttaa atctgttatt tgagtaggga
     8041 ttaatgtacg attttctttt attgtgatag gacttgatac tattttcttt agagacaaaa
     8101 gttttttgat taaattatct gtttgaacct cattgttaaa atctagataa agcattatat
     8161 tagttgcatt tcttatgtct tccatttgaa gtaatttttc agttattaaa tttgaattac
     8221 taagtacgaa atttgcattt ttattttttc ttgattcaat aacttttttt ctaaattctt
     8281 ttttcataaa ataattcctc ccttaatgtg ttataatgtg tacagcaatt gtatttaata
     8341 taaattttag tataatttat tgtaataaag caatatattt gggtaaatgt aataaaaata
     8401 acagctagtc atataataat aatagtttat gtaaaaggag atgtatcttg tgatttcaaa
     8461 gaagaaagct atttttttag gggtgatttt aataattata acagctatgg ttacatcagc
     8521 atttcagttg acattgggga ataaagttgt aatatcgaaa gaactctatg aagactataa
     8581 aaaatatgat aagttgttag gactagaatc tattataaaa caagattttt ataaaaaagt
     8641 atcagatact gatttggtaa atggagcatc aaaaggtcta tttctaggaa caaatgataa
     8701 gtattctggt tattatacta aagatgaaat ggaaaatcta attaatgata gtgaaggttc
     8761 ttatgttggt gtaggtatgt atataggggc atcaaaagat ggtggcttag tagtagttcc
     8821 tatgaaagat tctccagcag aaaaagcagg tgtaaaatca ggagataaat tagtaaaagt
     8881 caatggaaaa tctgtctcat ataaaaattc agatgaagct gttcgtatga tgaagggtaa
     8941 aaaggggaaa acagttgagc tgactatatt aagagaagat aaacaattga actttaaagt
     9001 aaaaacagac caaatagttg agaagagtat agagagtaaa gtaattgata atgatttagg
     9061 atatatagaa attacccaat ttatatcaag tacatatact gactttgata aagctttaaa
     9121 agaattaaaa gcaaaaaata tcaagggatt agtaatagac cttagaaaca atcctggtgg
     9181 aatgctagat atatgtaaag aagttgctga tgagttaatt ggtgaaggaa ctatagtata
     9241 tacaaaagat aataagggaa atactgagta tttgaaatct gataaagaaa aattgggact
     9301 tccaatagta gtattgacta atggagaaag tgcctctgct gctgaaatat taacaggtgc
     9361 aatcgttgac aataaagaag gtatttctgt aggtactaca acttttggta aagggttggt
     9421 acaatcagtt gtaagactga aggatggaac aggttacaaa ctgactactg cccaatattt
     9481 tacacctaat ggagattata tcaatgaaaa aggtataaag cctacaattg aagaaaaaga
     9541 tgaaaataag caattagatg tagcaactaa gtggcttaga gaacagattg ataaatagga
     9601 tttaattctt ataaacttat attataaaat aaagagtctt ataaaaggag tgtctcaaaa
     9661 tgaaattttt agttcatgag gcactttttt gtaagatgtc gaatgtattt tcatcacttt
     9721 aacaatgaaa aagaggctta tctctatttt tggacagcct ccataacttt atatttaacc
     9781 tatttattaa ctttgtactt acatatacca tttatataag tttcgtgtat attaaagccc
     9841 ttatctgtta ctataaagtc tgcatccata ccttgatata tggagccaca gacattatca
     9901 atattacaac ttaaagcagg tacatatgta gccattttta ttgcatcttc taaagtcgat
     9961 atattccaat caaccatatt tctcacagcc ctgtccatag ttaatacact accagctaaa
    10021 ctcccactac ttaatcttgc aacactattt tttacattta cattaaactt accaagagtg
    10081 tattttccat cctccatagc accagccctc atacaatctg taattaaagc tatgttatta
    10141 ctagacttag catccatcat tattttaata gctatggggc ttacatgatg accatcacaa
    10201 attaattcag cataagtatc ttttagtacc atagcagctc caaccattcc aggttctcta
    10261 tgatttaaag ggctcatccc attatatgta tgtacaaaca ctttagcacc tgaattaaca
    10321 acttgtttaa cttgttcaaa tgtagctgaa ctatgaccaa gagaaactga gatattattt
    10381 ttagttgcat attttgttaa ttctattgaa cctttacgtt ctggagcaat tgctattttt
    10441 cttattaaat ttttcgattt tttttgccat aattttaata tattgatgtc agggtcttga
    10501 aaatattttt cattttgagc acccttatat ttttctgtaa aaaatggacc ttctaaataa
    10561 atcccttgta tttttgcccc ctcaacttta ttgaaactat tgcttatatt ttctacagaa
    10621 tcacttagtg tatctacgga atcagttaaa gtcgtaggta ggaaagatgt tactccatat
    10681 tttaataagc cttttgaaat cgtgtttatt gcttcaaatg tattgtccat gacatctgca
    10741 cctaaaagac cgtgaatgtg agtatcaact aagccaggag caatatattt acctgaaaaa
    10801 tctataacat tatcagtagt actaggtctt tcttgctgaa attcaccgaa tttgccatta
    10861 attatactaa ggtaaccttt ttctttggtt ccatgtttca taaaaaattt atctgcataa
    10921 ataaaacttt tcaaaactgc ctccttataa ttatgttatt attgtgttat tgacgtttat
    10981 aatatcatta agttttattg aaatttattt aatttactat cttacatgcc ttactctata
    11041 tacaaattta tcgcttctag caacactcaa agtaaattca atgactatat tatcatcatt
    11101 gtatgttgta cgaagtaatt ttagacaagg tgaatctcct ggtacttcta atagcgttgc
    11161 ttccttgtca gatattattc cagctgaaaa ttcttcatca gcaactttaa taacctcacc
    11221 ataatcttct ctaaatattt catataaagg tttttgttcg attttttctt ttgtcaatcc
    11281 tataaattct ttcataggta gatatgttct ttcaagcatc ataggaaggt cattagcaag
    11341 tcttaaacgc attattttta ttattttttc accaggagtt atacctaatt tttcagaaaa
    11401 atactttgta ctttctacta cctcaaatga taatactttt gtacttggct tttttccttg
    11461 tgttctcata taatctgtaa agctataact ttccattaag ttttgagaat tttgactgag
    11521 tgcagcaaca aaagttcctt ttccatgtct tttatatata tatcccatat gttctaactc
    11581 agccatagct agtctaactg tagttctact tactccatat tcatcacaaa tttttctttc
    11641 cgaaagcatt ctatcgtttg gttccatctc attttcaatc ttagttctta atatttcaac
    11701 aagctggtca tatattggtc ttttatcatt tctttttatc ataaaaatat agtcctttct
    11761 aagtggttat taccacaatt tgattataga atatagaatt atttatgtca atgaataaag
    11821 tttaatcaaa actcaataag taagtctatt aatttattat aaaattatgg tacattgaca
    11881 aaacaagtaa aataatttat attcaaattg gtaataacca ctttgggtat attgtagacg
    11941 aggtattttt tattttacat attaatttat tgtttagaaa agtaaaaaat taataatgag
    12001 gagaatctag tatgattaca gtaataacat ttaacccatc tatagataga atgtataggg
    12061 taaataatat aaacataggt gaggtgcaaa gggtagtaag taagaatgca actgctggag
    12121 gtaaaggatt aaacgtagct aaggtatgta aaattttaaa agaaaatcct ttagcgatag
    12181 gatttctagg tggatttaat ggagaattta taaaaaatga acttaaaaaa ttagagatac
    12241 aaaataagtt tacaaaaata gagcaggaaa ctagaaattg cttaaatata attgaggaca
    12301 ataaagttag tacagaattt ttagaaaaag gaccgatagt agaaaaaagc gatttagaga
    12361 gatttgaaaa tgatatagag gaagtattaa aaaatactaa aatactagtg gcatctggta
    12421 gctattgtga gaatatgccg atagactatt atgaaaagat aggcaatata tgtagagcaa
    12481 ataatataaa atttatatta gatacatcag gagaagcatt aaaaatagct ttaaaatcaa
    12541 agccgtattt aataaagcca aatatagatg aaataagaca tttattagga ataaatatag
    12601 aatcaagaga tgaaattatc ttatcaggaa aaaaacttat tgaaatgggt gcagagaatg
    12661 tatgtatatc cttaggaaaa gatggtatgg tgtatttaaa taaagatagt gtatatgatg
    12721 taaaagtacc agttgtaaag tgtataaata ctgtaggtag tggagatagc acagtggctg
    12781 gatttagtgt aggtattcta aggggttata aaatagaaaa tttacttaaa tttgcaaatg
    12841 cttgtggtat aagtaatgct ttaaatatgg aaactggatt tgtagatttg agagaaattg
    12901 ataaatatca agatttagtt gaagtaaaaa agttaagtta agtatattta atgtgaatat
    12961 actaagttta atgttgttgt aatagattta aaatttaata taaaagatat agtcttatat
    13021 aaaaataggt tagagaaatt catctttaac ctatttttat aataaatatc tattttactg
    13081 aatttataat tttttctagt aatctttgag ccttgacaca ctcttctcca ctaatagctg
    13141 gttttatatt attttctata caatcaataa aatgatttga tatatcttca aaaccctttt
    13201 gttttaatat atttacccat gcaccacttt gttttaaaat taggtttcca ccttcttcta
    13261 tttctagtac attaagattc ttaattcttt gaatcctgcc ttttgaaaga atttcaactt
    13321 gttctaactt tgttccagaa tctctatgca tagaggtgtt tatagagaaa tgtttacctt
    13381 taagtttatg acttacaaat ataagattct tattgtctgt taaaaataaa tcttctccac
    13441 taatttcaac atcttcattt gctaaccata aagcagtgtc aataacatgt atataatcat
    13501 ctataagggt actgtcgaat ctaacatttc ttaaggaatt taatccatgt ttacatatat
    13561 tgatagatac gatttctgta gcattatttt taatttcttt gtacatagga cagaatctac
    13621 ggttgaatcc aaccattaaa tttaagtttt ttttcgtact taattcgata agttcttctc
    13681 cttgatttac tgttgaagca agaggtttgt ctacatatac atgaacacct aaatttaata
    13741 atatttttat aatttcgtag tgtgtttctg tagagctatg tagaaaaatg caatcacatt
    13801 ttttagctaa agactcaata gaatcgaatg gcatgattct ataatcacta cagatttttt
    13861 ctcttttaac tttatttgga gtaaaagctc ctacaaactc aaatctctca gatttagtta
    13921 atataggtaa atatgccttt tgagctatgg agccaagacc tatcattccc atttttatat
    13981 ttttcataat actttctcct aaggttttta tatttacaga attgattata attcagacac
    14041 gtaaatttgt aaactttgta tcaaaaaata aacttaaggc ttatataagt aaattagcaa
    14101 aataagaaat attaataaaa taattttgta ggatatctat aaaattgatt tatataaata
    14161 ttttatctat tgacaaaaaa ttcaaattaa tttatatttg gattagtggt aattaccact
    14221 ttggtctgaa caagttttta tcgtaaaata aaaatactaa agtatctaga gtatgatttt
    14281 attaaatttt taaagacaaa ctataaaaat aatcaaaata tatagagtat attttatagt
    14341 gagtttaagt agtaataaaa aatctaggca agaaattaaa agaagggtat atttttttat
    14401 accttaaagt ggttataacc tcattaccaa aaataaatgt tgtaagtatg aaaggagaat
    14461 gaaaatgttt aagttagaag aacaaaaact aaaagattta ggagcaataa taacaactaa
    14521 tgaaataaaa caacaacctg aattatggtt agaaacttat gaaatctata aatctaacaa
    14581 agaagaacta cgtagattta tagataaaat aagtaataat catggacagt ttagagttat
    14641 atttacaggt gctggaactt cagcttatgt aggaaactca atactacctt atcttaagaa
    14701 taacaatgac ataagaaaat acatatttga agcaatacct actacagata tagtttcaaa
    14761 cccatatgat tacttaaaaa aagatgtacc aacgttatta atttcatttg cgagaagtgg
    14821 aaatagtcct gaaagtttgg ctgctttaaa tttaggaaat aagattgtag ataatttcta
    14881 ccatttagct ataacttgta atccacaagg agaactggct aagatgacta aaaacgacga
    14941 aaataattac ttgttattaa tgccatcaaa atcaaatgat gaaggctttg ctatgacagg
    15001 aagtttttcg tgtatgatgt tatctgctat gttaatattc gatagcttag aagatgatgt
    15061 agaaaagtca tatataaatg caataattga aatgggaaga aatgttatag atagaaaaga
    15121 tgaaatacat gagttaataa ataaagattt tgatagagtt gtgtatttag gttcaggagg
    15181 attaggtggt ttgacacaag aagcacaact aaaactttta gaacttacag cagggaaaat
    15241 agctacggtg tatgattctc ctatggggtt tagacatgga ccaaaatcat ttattgatga
    15301 aaatacactt gtatttgaat ttgtatctaa ctgtttatat acaagaaaat atgatttaga
    15361 tgttttagag gagataaaaa gggataaaat tgctaaattc acttgtgcag tgtcagtaga
    15421 aaatgaaaat aacttctcag gaacaaagtt tgagtttaaa gaaaagtata ataaattacc
    15481 agatgtgtat cttgcaatgc catacatatt gtttgcacaa actatagcat tgtttgtatc
    15541 ggtaaaggtt ggaaataagc cagatacgcc atcagctaca ggaacggtaa atcgagtagt
    15601 aaaaggtgta acaatatatg aatattagaa gagaagatta aaatattata actaaataat
    15661 ttgggagggt attaatgtgg cattagtaac aacaacatca atgcttaaaa aggcacaaga
    15721 tgggggatat gcagtaggag catttaatgt agaaaatatg gaaatggtaa ttgctgttat
    15781 agaagctgct gaggagttaa attctccagt aatattacaa actactccat ctactgtaaa
    15841 atatgcaggt ctcgattatt atctagcaaa tgcaaaggtt gctgcggaaa gagtaaagat
    15901 acctgtagca atgcatctgg accatgggtc aagttttgac cttgcaatga aagctttaag
    15961 aagtggatat acatcaataa tgatagatgg ttctcatgaa agctttgaaa ataatatatc
    16021 tatatctaaa tcagtagtaa aagcatgtag cccttctctt ataccagtgg aagcagagct
    16081 tggaaaagtt ggaggaaaag aagatgattt agatggaggg aatagtaatg attatacaaa
    16141 tccaaaagag gcaaaagagt ttgtcgatag aacaggaata tcttcattgg ctattgctat
    16201 aggtactgca catggattgt ataagggaga acctaaaata gatttagata ggttatcaga
    16261 aattaaaaat gtagtttcaa tacctttagt attgcatgga ggttcaggaa tacatgatga
    16321 tattataaaa gaagctataa aaagaggtat tgcaaaagtt aactatgcaa ctgaattaag
    16381 aattgcatat agtaaagggg ttaaaaaagt tttaaatgaa gattctgagg ttatagaccc
    16441 taaaaagtat ggactagcag ggcttcaatc agtaaaggat tttgtaaaag aaaagattaa
    16501 agtatgtggg tctgtaaata aagtataaaa aataaaatat cctaaattta ttttagataa
    16561 gtttaggata tttgttatat gaaaatttta tattaattat gggtaaatgg tgtatttttt
    16621 tcatttaatt gatgaaaaat atcagctttt attggtaaag tattacatat ataattaaaa
    16681 tataataaaa attgaactat atattttatt attatgaaat atggatatta aattttagtg
    16741 ctaaaatgta cgaggatata atattctata aatgaaaaca aaaggttgac tttataagaa
    16801 aaaatttttt atgctacttt gaaaaataca ataataacac tgagagaggt ggggatatga
    16861 gagaacgcat tattcttgaa gcattaaaaa atagtagtgg ttatctaaac atagattatt
    16921 ttgcagataa gttgggggta tctactcgaa ctatcagaaa tgaaataaaa aagatagaaa
    16981 ctataaaaga atgtaatggt ttcaaattag aatataagtc taaactagga tatattctaa
    17041 atattaaaga ccaagataaa ttcgagcatt acctaagaaa cttaccctca gatttagttg
    17101 aaaatccaga acaacggtta gaatctataa tagtagaact tttagtaaat gaaggttata
    17161 aaactataga acagttaagt aaaaaatttt tagtaagttc atctcaaata aaaaatgatt
    17221 taaaaaagat agatgaaaaa ataaaggata cagaattaaa attggagcga aaggctcatt
    17281 atggaataaa aatagaaggg tctataaaag aaattcaaag tatactggtg gatagttact
    17341 ttaaagggaa tagaaatata acaaaataca gaaataaatt tatagataat attaaactag
    17401 ataatatcag aagtacaata aaaaatgtac ttaatgaaca ttatttagaa gcaaatttaa
    17461 ccgaacttga ggaaatatta gcacaaatta ttatattgta tataagagtt agcactagag
    17521 tagtaagaag tcctagtgat ttagaattat atacagaaga tttaattata gacgaattat
    17581 tagataaaat ttttggagat aagaagtata gccttactta tgatgaaaag tattatttaa
    17641 agcaacttat aaaattaagg actaaggata agaaagccac cataaagaac attgataaga
    17701 ataaacttca agatattatg tgcgaatttt ttagagatat cgataaaaag tataactcta
    17761 attttttaga agataaagag ttccttaact tattttattt acatattgca tgtttaatag
    17821 aacgtataaa gaagaatcac aagataacaa atcctttttc agttaaaata agtcaacagt
    17881 atccaactgt ttttaattta tctattcaat tttcaaaaat catagaaaat gagtatcaca
    17941 taaaaataag tcaagatgaa ataggtttta tagctactca cattgctgtt ccttttgaaa
    18001 agagagaaga ggctaatttt aataaaaaat ataaaatagc aataatatgc tcttctggtg
    18061 gaggcagtgc atttttgata aatttgagac ttaaagaaat ctttccaaat gctgaaataa
    18121 aaaacttttc attactagaa gaaaaggctg taatagaatt tgcgccagat ttgatttttt
    18181 caataacaaa tcttttattt gaaataaatg ctccagttat acttataaat gaaattttag
    18241 acgaactgga ttatttggat attaaagaaa gttttagatt tgtagaccat ataggaaatg
    18301 tatcaagccc aaaacaatat atattaggtc tgtttgataa gaatcacttt aggtgtgtaa
    18361 aagagaaaac caaatataaa gatatccttg atagtatgtc gcaatgtata gttgatgagg
    18421 gagcttgttc tccgacttat cccaaagatg tgtgggaaag agaaagctat ttgagcacta
    18481 tttacacaaa tggtgtagcg atacctcatc caattgaaat gacaggtaat aaaaatatta
    18541 tatcagtagc cttgatacaa actgatatta attatgaaaa tagggtacca aagattattt
    18601 tcatgatttc attaataaag ggaaatctag agcttcataa gcaaatatct aaatatttaa
    18661 gtaagattat gacagataag gatatggtag atatgctaaa taagtcacaa tcttatgagg
    18721 aatttatgta taagctaaaa atctatctgg gaggttagac atgaatatag agttttttaa
    18781 gaggttatca gaagcagatg gtatagcatc taatgaagaa gaggttagac atgttttatt
    18841 ggaagaacta agagcttata gtgataaagt tatatgtgat ggtctaggga gtataatttt
    18901 ttctaagata aaagatgaaa gtgcaccaaa cgtaatgatt tgtgctcata tggatgaagt
    18961 tggatttatg gtgagaagta tagacaaact tggtatgatt cacctaatga caataggagg
    19021 agtaaaacct cttgcacagt ttgtacaaaa agttagaatt acaacaaaag aaggaaagaa
    19081 aatccacgct atcataaatg ctacatataa taatggtaag gctgaaaata tatatgcaga
    19141 tattggagca tatacagaag aagatgtata caacctaggg attaatgttg gggatatggt
    19201 aacatataca acaaactttg aggaatttac tttgccagac cgattagtgg gaaaagcttt
    19261 tgatgatagg ataggatgtt ttgtgatggg agaagtttta aaggaactta agaaagaaaa
    19321 tctaaattgt aatattcatt ttgcagctac aagcagtgaa gaagttggaa taagaggggc
    19381 aaagacgtca acgcagttaa taaatccaga tatagtattt gtaatagatg tagcatgtgc
    19441 taagaatgag tttgtgaggg actatacaaa tcagaaacaa attgataaag gtgtaatgct
    19501 aatgcataga gataggactt tagtccctag tagaaaaatg atagattatg taatgaaaat
    19561 tgctagtgaa aataatatac cacttcaaca tgatatgttt gaatcaggtg ggactgatgg
    19621 aggagaagca catctcgtaa atgaagggaa accttgtgta gtaacttgtg taccagttag
    19681 atatggtcat tgtgcatttt caatagtaag taataaagat ttggaaaaca ttataagatt
    19741 gtatacacag ttaattttaa attttgatga agaaaaatat agacatataa gaaattttat
    19801 ctaaaggaga ataagaaaat gtcaacagaa gtacaaataa tggaacttat atcaactgca
    19861 ggagagagta aatcgaaagc ttttgaggct ttaaaaaaag ttaagtcaaa agattttaaa
    19921 ggagcaaaat tgttaataga ggaggctcgt aaaatagata ttgaagctca caggatacaa
    19981 acagaattaa taactcaaga tttaaatgaa gacgttgaaa gcccagcaat gagtctttta
    20041 atggtacatg ctcaagacca ctatatgact tctcagctag caaaagactt gatagaggct
    20101 ttgatatatt gtcttgataa gtaattgtaa tgtagaaata gattatttat gtaaaaattc
    20161 ttttaaaagt ttagatgctt ttaagagata ataaaaatac aaactttagg aggaattatt
    20221 atgaaaatat tattatgttg tgcaggtgga ttttctacta atatgttaat gcaaaatatg
    20281 aaaaaggtgg taaaagagag tgctaaactc aatgaaaaag attttgagtt tacagctata
    20341 ccagcagatt caatagaaag tgaagttgat gactgggata tagtacttat agggcctcaa
    20401 atagctcaca aaatagattt cttaaaagga atattagagc caaaaaataa accttttgca
    20461 gtaatagata aagatgtata tggaaaaatg gatggagcta cagttatgaa gttggcatta
    20521 ataacttata agaaacatca attagctcaa ggataataaa aataatgagg gaaaagggag
    20581 attagatatg ttttcaaaat ttgaagcatt tatgaataga gtatttatgc ctttagctca
    20641 taaggttgac aatcaacgtc accttggttc tataaaggct ggtatggttg ctatgacacc
    20701 attcacaata ttagggagta tatttgcaat acttccagca cttccaaata tgcttggaga
    20761 agataatcca ataagcaaat tcatactgaa caattcagca atattggact taccagtaag
    20821 tctttccata ggcttaatag gattatatgc ttgtatgtgt attgctttta atctggggaa
    20881 ctactataaa ttatatatac caggttgtgt tgcactatca actttttctt ttttattcct
    20941 agtagcatca tttacaccag atggagcttt agaaatagga aatttaggag ctaaaggact
    21001 atttacaggt atgatagcag ggcttggttc tgtggagtta tataattttt gtaataagaa
    21061 gaatatcaca ataaaaatgc cagaaaatgt acctgatttt gtatctaggt cttttgaatt
    21121 aatacctact actataatag tttgtggaac ttttatagtt gctcgtttta tttcattaaa
    21181 aatatttgga gaattacctc cacagatact tactcaattt ttatctccat tagtaggaag
    21241 tatggataac ccatggtcag ttcttgcact taactttgct atatgtacta tattcttttt
    21301 tggaatacat tcatcagtat ttagccctat aactagacct ataatgatta catttatagc
    21361 tgaaaatata gcagctatgc aagctggaga aacaatacct catttcttta cagcaggagc
    21421 ttcaagtgca ttctttggat ttacaggatg tggaattagt ataggttgtg taattgcatg
    21481 tatgttatca aaaaatgctt cctataagaa gattggacgt gtatcattat tccctgcatt
    21541 atttgggata aatgaaccta tattatttgg agcaccaata atattaaatc caataatgtt
    21601 tataccacat gtatttggtg gagctataat aggtacacta ccaatgtttt ttatgcactg
    21661 gggatggtta gcaaagccaa tatttgaccc tccatatgta ggagtattct tagaaggatt
    21721 tttaactaat ggagattggc attctatatt agcaaatgca ttgcaattag tattaacagt
    21781 tttattatat tggccattct ttaaaataat ggaacgtcaa caaaataata aaaaacaaaa
    21841 agaagaaact aagaatatat tctctaaaga agaagaagac ttattggatg atttaggatt
    21901 agatttctaa tattttgtta tgaataatac aatagagaga ctagtatcct taatcgataa
    21961 aagaggtgtt gacgctcttt tgattaaagc aaaaaatact aaaagatata taggggcatt
    22021 atcaggtagt ggtgtatatg tgcttgttac taaagataaa aattatcaag ttctagatgg
    22081 cagatatatt gatgaagcag ataaaaaaac gactggtttt ataaaaagga ttgtatctca
    22141 aggttcttat ataccaacta taattgagtt attaaaagaa ttaaatataa aaaagttagc
    22201 gatagaaaac caagctataa gtatacaaga gtatctttca ctaaaaaatg caggttttaa
    22261 aatcaattta ataacaaatg aattatggaa gctaagggct ataaaatcta aagaagaaat
    22321 tgaacttatt aaaaaggctt gtgagattac agatgaagta tttgatgagg ttatttctga
    22381 aattaaaaaa gatatgactg agctagaagt atcagctcta attcagtatc atgcattgaa
    22441 aaaaggtgca agtggaatgt cttttgagac aatagtggta tcaggagaaa gaggtgcaat
    22501 gccacatgga agacctacaa acaaaaagtt gaaagtgaat gaagctataa caatagattt
    22561 tggtgttgtt tatcaagggt atcaatcaga tatgactcgt acaatatcta taggtaaacc
    22621 tcctaaaatt attaaagaga tatatgatgt agtattggaa gcacaattat ctgcaataga
    22681 gtcaataaag gaaggaacta gggcaagtga tgttgacaaa gtggctagag aaattataga
    22741 taaacatgga tttggcgaat attttaatca tggactgggc catggtatag gtcttggaga
    22801 tggtgaagtt cccactctaa atccaaatag tgaagatata ttagtggagg gaatggtaat
    22861 gtcttgtgag ccaggtattt atattccaaa tgtaggggga gttagaatag aagatgacat
    22921 tgttataata gatggaaaag gtgttccact caataagaca tccaaagagt ttataatatt
    22981 aggagaataa aattatgaaa tataatttta ataaagtatt aaatcgtaaa gaaggaaatt
    23041 gtaggaaatg gagcaaccac gtaattaaag aaaaattcgg cttaaatgaa gatgcaattc
    23101 ctatggattt agctgatatt gattttgagt gtgctcctgc tataaaagaa gctatcttaa
    23161 aaagagcatc aataggagat tatagttata cttttatagg agatgacttt tatgatgcta
    23221 ttataaattg gaataaaaga agatttgatg tatatataga aaaggattgg ataaaattaa
    23281 cttttggtac tgtttcaacc cttcattata tagtccaagc atatactaaa gaaggagaag
    23341 gtgtattgat aaatactcct gcttatgacc cttttgcaga ggctgtatta aataataata
    23401 gaaagttgtg ttgtagctca ttaaaactag ataacaatag atattattta gatttagagg
    23461 atatggaaaa tcaaatgaaa tacgagaata taagattgct tatattctgt agtccacaaa
    23521 atccatcagg tcgtatatgg acaaaagaag aactatatca agtgtcagag ttatgtttaa
    23581 agtataatgt aattttggta agtgatgaaa ttcatagaga tataatattt aaagattata
    23641 gatttgtatc tttgtggaat gctcatccag aaatttataa aaatagtata atctgtgtat
    23701 ctcctaataa agggttcaac ttaggtggat taaaaacatc atatgtatta ataagagatt
    23761 taaaaattag ggagacttta ttagaaaggc taaaaagtaa ttcaataact tctccaaatg
    23821 tatttgcaat accagcaatc gttgcagcat acaatgaaag cgaagaatgg ctagatgcta
    23881 tgactagtta tgtagaggag aattttgaaa tagtttatga tttctttgaa actcatatac
    23941 caaaagccaa tgtgatgaag tcagactctt cttttttagc ctggatagat gtaagaggag
    24001 tgtttagaaa tgaagaagaa tctaaagaat tttttagaca tgcaaactta actatggtag
    24061 ttggaagtta ttttgtgaaa gatggagatg gatttattcg tattaatgtg ggatgtccta
    24121 gagaaacatt aaatgaggct ctaaatagaa tcaaaaaaac gtatataagt atgtattgtt
    24181 aaattataaa aaaggtatat atggtttata taaaaacaca ttatttagtt atcaaatatt
    24241 actggaattt tgcattccag taatattttt tgattaagga atattattga agaatattaa
    24301 ataatattat ttcatatcct tatattaact tatataaaaa tattttatta ttttgggaac
    24361 gggttagcta acctttatct agtttatatt tatactgtaa gaagcagtaa aaacttttcc
    24421 tttactcagt ttattaataa acttcttatc tttataaatt ttattagaat ttatattatc
    24481 agcaagacca taccaaggtt ctatgcataa aaaaggagca gtagaattgg tttcactata
    24541 ataaggagtc caaataccaa ctagagggaa atctttcata gatatagtta catatttact
    24601 accattatta ttacatatag atacttcatc tatgttggtg tatatcaaag catcattctt
    24661 gaataattct ggatttaact gtaagttttt taattttcca atagatatta tatcatccgt
    24721 aaaagaacca cttaaattga ttttttctac atcatttcta cttttaaatt caaggtagtg
    24781 tttagaaaaa tcattttgtt cgcaagaagg aaggttgaat gcaggatgtc cacctataga
    24841 aaagaatata tctttagagt cagtattttt cacaatccat tctacctcaa tgctgttatc
    24901 atttaatgta tagtttatga ttagctcaaa agaatatgga tatttcttta aagtagattc
    24961 attatctgtt aatttataag atacgaatgt atctccttta tctacaatct caaagtccat
    25021 atctctagca aatccatgct gagtcatact atataaattt tcttctatta ttgtttcatt
    25081 atcaaaaagt ttacctacta taggaaacag tataggtgat tgtctacccc aatatttttt
    25141 gttaccattc caaagaatat ttttgtcgta ttttttagaa aatattttag ttagttcagc
    25201 accaaaactg cttgattcga taattaaatt ttcattttgt aatatattca tttaaaatct
    25261 cctttattat attttatgag tatattatac tataaagtat aaagattatt aaattttatt
    25321 tgatattata tttgaaaggt agattttagt atattttgaa aattttgttt tgaaaatttg
    25381 ggattattta agaaattttg tattataaat agaaggaaat agataaattg tatagaataa
    25441 aatatattaa gataagttaa taatgttaaa tgggaagtga gtttatggaa aaaccaaata
    25501 caaaagttgt taactttcaa gagtataaag acagaaaaaa caaaagagaa gtaaatagag
    25561 acgaactttt aaagcttata aaaaaaattg taacttcaaa atagggagtt gttaaactcc
    25621 ctattttaat gggtttcata ataattaaga cattctaagt ccatctatat aatctttgaa
    25681 ttgtgagaaa tctgattcac tacttaattc tatagtttct gaaccttccg tataaaaatt
    25741 cttaagatag atagtataat tattatcaac acaatcatga taaatacatc tatatccatc
    25801 ttcatacaag ctttttaact tagaaaaatc atccacaata tcatctcctt aaagttcttt
    25861 taaatatatt tttacaaaaa gcatctaatt gtatgcaaat tgaaaatgta aaataaagga
    25921 aatgaatata aaatgtagaa taatttttat atgtatgtaa aaataaaaag aaaaagttaa
    25981 aatttttaga aaataagagt gttttttatc taaaaagaga gtttaaaaaa taaaaaaatg
    26041 ttgggaggat aaaaaatgag tttattagaa agtatatcaa aaaacatata ttccctagat
    26101 agttcttcta ttgaaaaaac aaagcagaga ttggataggc ttatacatcc aactggaagc
    26161 ttaggaaaaa tagaagatat ttgcatgcaa ttagcaggaa tatttggtaa tgaaaatttt
    26221 gatacgagca aaaaggttat aattgctttt gctggagacc atggtgtata tgaagaagga
    26281 gtagcacctg accctcaaaa tataacaaaa ctacaatttc caaatttctc taaaggccta
    26341 tgtggagttg gagtaataag taaatttgta ggggcagatg ttgtagctgt agatgtagga
    26401 ataaactgtg atgaaaaact ggatggagta ttagattaca agataagaaa agggacatca
    26461 aatatggcaa aaggtccagc tatgagtaaa caagaagcta ttagatgttt agagataggt
    26521 attgaaatag cagaacagtg tatagaaaaa gattataagg ttattggtat tggggagatg
    26581 ggtatagcaa atactactcc gagcacagca ataatatctg taatttcagg ttgtgacccg
    26641 ttagaggtta ctggtatagg tgcaggtctt aagaaagaaa gactaaagca taaggctgaa
    26701 gtaattagaa aggctattga aattaacaat ccaaatccaa ctgatggagt agatatttta
    26761 tctaaagtag gaggatttga aataggctca atggcaggtg ttatactagg ttgtagtgct
    26821 aataggattc cagtagtaat tgatgggttt atttcttatg cagcagcatt aattgcatat
    26881 aagataaatc caaaaactag agaatatatg atagcatctc atttatcagc agaatcagga
    26941 actaaaagag cattagacat attaaaatta gaccctctat taaatatgga tatgagatta
    27001 ggtgagggaa gtggagctgc tttagcattt aatataatag aagcttcaaa ttatacttac
    27061 aaaaatatgg caacttttga tgaaattgac atgggaagat aaatataaaa atttaatatg
    27121 taggagtgtt ttgatgagta aaattattct agtaacagga ggcgctaggt cagggaagag
    27181 taactttgca gaaaaactat gtttggacag aaataataat acagcttata tagcaacctc
    27241 tattccattt gatgatgaaa tgaaagatag agtaaaaaaa catcaagaga atagacctag
    27301 aaactggaat acctatgaaa tatatgaaga tatatattcc ataattaaag agatatataa
    27361 agaacatgaa actgtcatac tggattgtgt aacattatta gtaaataatc taatgtttaa
    27421 acacaattta aatatagaag agtctagtca agaagaaata aacaaattag aacaatacat
    27481 aaaagaacaa gttgtcaaac ttattgaaga aatagaaaaa acaaatttat actttgtatt
    27541 tgtaacgaat gaattaggta tgggaattgt tcctgggaat aaacttagta gaatttatgc
    27601 tgatattgca ggtcgcataa atcaatatat agcgtcaaaa agtaatgagg tttattttgt
    27661 agttagtggc atacctatga agataaagga gtagtatgtg attatgaaaa gatttatttt
    27721 aatactacag tttttgacaa ggatacctat aaaattaaat gtaggatttg atgatgaatt
    27781 ttataagtca atagtatatt ttccacttgt aggttttgtc ataggtatat tatcctattt
    27841 agtaggatgg atttctatgc ttttatttga accatttata gcttctataa taataactct
    27901 agctggcgtc ttaattactg gaggacttca tatagatgga ttaggagata catttgatgc
    27961 aatatatagt tataggaata aagaaaaaat gcttgaaata atgaaagatt caagattagg
    28021 tacaaattca cttctagcaa ttatgtttgt acttttatta aagattggat tcatctacga
    28081 tataataagt aataattcat tgtgggttat aatttttatg ccaatgattg caagattagg
    28141 agtaatgtta ttgacatata aaactgtgac accaagggaa aaaggaatgg gaaatttatt
    28201 tataggaaaa ttaactacaa gtatgttaat aactgctata atatacacat tactgatagt
    28261 tgctcttatt actaaattta tatttttatt acctaatata gtgttgatta aagtattagg
    28321 ttcaactata gtagtttttg tatttataat attattcaaa aaacatattt ataaaaaaat
    28381 tgacggagta acaggagata tattaggttg tggaatagaa ctttcagagg ttgtatattt
    28441 aatttacata tatttgttaa attttatgtt tttttaaagt aaaaagtggt gataatatga
    28501 taagattaat attaataaga catgcactaa caaatgataa taaaaaagga aaattatcag
    28561 gtcatataaa tagttgtatt agtgaagaag gtaaactaca aataaataaa attactagat
    28621 atctgagtaa cgaaaacata gataagattt atacaacacc atctacacgt actaaagata
    28681 caatagaaaa aatatcaaaa ttgaaattat tagaaataga agaaaaagaa actttaagag
    28741 aaattagttt tggagatttt gaaggaataa cttttgaaga aattaaaatc aaatatccca
    28801 atgagtttga aaaaatgata aaagaaggta acaactatag gtatccaaat ggagaaagtt
    28861 tgatagactc ttataaaaga gtggcaaaag aaattgataa tataatttta gaaaatgata
    28921 gtaatttagg ttcaaagaca atacttatat gttctcatgc aggaacaata agaaatataa
    28981 taacacattt gatttctggt agctataagt atcattggaa ctttaaaatt gataatgcgt
    29041 ctattacagt tttagatgtt gatagtggat ttactgtaat agataaaatg aattttacag
    29101 acttcatata atttaatcaa aatcaaatat tacaatctgt gtttgtaata tatctgtaaa
    29161 attagcaatg aaaatagaat attagattaa aattaggttc ttaattagat gttaaaaggg
    29221 aaaaaggtta gatgcctttg cagcccccgc tactgtgaaa ccaacgaaac tataaatacc
    29281 actgtcagtt tgatgggaag gttatagaag taggatgaag ttaagccagg atacctgcct
    29341 aatttaattt aaaacaagag gtcttcgggg tgaaagattt ttaattaata tatataaaag
    29401 tcctatcttt agaagcatta ggactttttt taatttggtt tttagtaaat tttaacaagg
    29461 agggatggaa agtggggaaa aaaataatgc tacaaggaac agcttcaaat gtgggtaaaa
    29521 gtgtcttagt agcaggtttg tgtagaatat ttaaacaaga tggttatact gttgcgcctt
    29581 ttaagtcaca aaatatggct ttaaattctt ttataactaa agaaggatta gaaatgggaa
    29641 gagcgcaagt atttcaagca gaggctgcga aaatagagcc aatagctgat atgaatccag
    29701 ttttattaaa accttctgga aataataaaa gtcaagttat tgtaagaggt aaagttgtag
    29761 gagaaatgcc ttcttctaag tatcatgatt ataaactgga gttagtagat gtattgaagg
    29821 aaacctttaa tagcttaaat tcaaggtatg atattgtggt tatggaaggt gcaggaagta
    29881 atgcagagat aaacctaaaa gacagagata tctctaatat gggtatggca gaaatagctg
    29941 atgcacctgt aataatagtc ggagatattg atagaggagg tgtgtttgcc tcattagcag
    30001 gaacaatgct tttattaaat gaagatgaga aaagaagagt aaaaggtgta ataatcaata
    30061 aattcagagg taaaaaagag cttttacaag atggtgtaaa gatgctagaa gacatcataa
    30121 aagtacctgt cataggagta gtaccttata cagatataaa aatagaagat gaagatagtg
    30181 taactactag atttaagaaa aaaatgaata aaagcgatat acacatagaa ataattagaa
    30241 ctccacatat gtcaaatttt acagatttta atatatttga gacacaagaa gatgtaagta
    30301 ttagatatgt agactatgga gaatctttag gaaatccaga tatattaata atacctggaa
    30361 ctaaaagtac tatagacgat ttaacatata taagaaaaag tggtcttgaa tcgcaaatca
    30421 aaaatttaca tagtcaaggt aaattaatat ttgggatatg tggtggatac caaatgttag
    30481 gtaaaaaatt aaaagaccct tatcatgttg aaagtgaaat tgaagaagtt gatggaatag
    30541 gtcttttaga tacagaaaca acgtttgaga aagaaaagac tacaacacaa gtagaagcca
    30601 ctattgttga atgtatcagt gaatatatga ataaccttaa aggaaagaaa ataaaaggtt
    30661 atgaaattca tatgggcatt acaaagcgag gtaatgatgt aaggagttta gatttaataa
    30721 ctaaaaaact tgataaagaa gtgaattata ctgaaggaag tgtaaataaa gaggggaatg
    30781 taattgggac ttatcttcat ggaatatttg atgaaataag ttttacaaga gcaattttaa
    30841 acaatataag aaagaaaaaa ggtttaggag aattagaaag caaagtaaac tcttttaatg
    30901 aatttaaaga taaagagtat gataagttgg ctgatttttt aagagaacat ttagatatag
    30961 agaaaatata tgaaattatg aaataatgat aacctttatt tataatttag gtaggaggtg
    31021 tattatgaaa aaaattttaa ttgcgggtac tagtagtgga gtaggtaaaa caacaatatc
    31081 attaggaata atgcaagctt tggttaagag aaatatgaaa gtacagccat ataaagtagg
    31141 gccagactat atagaccctt catatcacac ttttataact ggaagacatt ccagaaattt
    31201 agattcttat atgttagatg atgaaaaaat aaagtatata gtaaataagt cctcaaaaga
    31261 tgcagatatt tcagtaatag aaggtgttat gggcttatat gatggttttg gaatagattt
    31321 agataattgt acaagttcac atacttcaaa ggtattaaag tcacctgtta tattagttat
    31381 aaatggtaaa tctatggcag catctagtgc tgctatggta cttgggtata gggaactaga
    31441 taaagatgta aatataaaag gtgttatagt aaataatgta aagactaata gccattatca
    31501 aataattaag agttctatag aaaaatattg taatgttgaa gtattggggt attttcctcc
    31561 aaataataaa ttctctttag aatctcgaca tctaggatta gttcctagtg tagaaatgaa
    31621 atcactaagg gaaaaatttt atactttagc agatgaaatt gagcactata tagatattga
    31681 tagaataatt gaaatatcag aaagtgatga atttgagagt gattttgatt ttcaaacttt
    31741 gatagaaaat aataaaataa aagaacatgt taataataaa tcaatagcta tagcatatga
    31801 taaagcattc aacttttatt atagagaaaa tatagagctt ttagaagaac ttggtttaga
    31861 aataaactat tttagccctc ttgaagatga atcagtgcca aatgcagatt atatttatat
    31921 tggaggaggt tttccagaaa tatttggaaa agaattagaa gcaaacgagt caatgagagt
    31981 ttctatatta gaagcacata acaataatat acctatttat gcagaatgtg gaggactgat
    32041 gtacctaggt gagaaactat tgaatcaaga agaacaagaa tttaatatgg ttggtatatt
    32101 cagtggaatt agcaagatga catcttcttt aaagagattt gggtattgca ttggtgtagc
    32161 caaggaaaat actcttttat ctaaaaaagg ggaatctatt aaaggtcatg agtttcatca
    32221 ttcaatattt gaaagtgatg aaaaatgtgt gtattcaatg agaaaagaac gagatggaaa
    32281 tatagtagaa gagtgggatg gtggttatag taaaggaaac actttggcta catatcttca
    32341 tactcatttt tataataact taaattgtat agaaaatttt ttgaacaaag gaaatgagta
    32401 aatgaatatt ttatctattt atatagggta tgtgacagac ttaattatag gagacccata
    32461 ttcttttcct catccagtga gatttattgg taaacttata aattttactc aaggtataat
    32521 aagaaaggtc tctaaaaatg ataggcagtt aaaattgggt ggatttatat tatggtttat
    32581 aactgttgga ataacatatt taacaactta tgctatagtg aagctattta gttttaatat
    32641 attattgagt attgtagtga atagttttat aatttataca acactagcca caaaatgttt
    32701 aaaagatgaa gcattaaaaa tctataatgt attaaaaact gaagatatag agaaatctag
    32761 gattcagcta tcttatatag taggtaggga taccactaat ttaagtgaac ctgagataat
    32821 aagagcaact gtagaaactg ttgcggaaaa tactgtagat ggtataattg cacctatgtt
    32881 ttatgcgttt attggaggag caccacttgc tatggcctat aaagctataa atacattgga
    32941 ttcaacagtt ggatataaaa ataaaaagta taaagatatt ggttttgcat ctgctaagat
    33001 tgatgatata gccaattata taccagctag aatatctgtt attcttatga caattggaag
    33061 tttcttttta agttatgatc atagaaactg ttttaaaatt tctattagag atagaaagaa
    33121 tcataaaagt ccaaattgtg ctttttcaga gggtgcagta tctggtgctc tagggataca
    33181 actgggaggt accaacgttt attttgggga aaaggtatat aaaccaacaa taggggataa
    33241 gcttagggag atagataaag aagatataat taaaactaat aaaattatgt atgcatcttc
    33301 atttgtatct atgttggtat ttacactaat atatgtgtta tgtaggatga ttttaatcaa
    33361 cttaattata taaattattg tttgttaact taagggggac atagatgaag gatttagggc
    33421 atggagccaa tgtagatgaa atggcaagat tatatgggaa agacccaaaa gaaataattg
    33481 attttagctc aaatataaat ccaaatgtat taccaaattt agaaagatac attttaaaag
    33541 gattagaaga gtgtaggaat tatccggata taaattatac aaacttgaga aaaaatatat
    33601 caaaatatat agatataaat ccagacttta taatacctgg aaatggtgca acagaagtga
    33661 tttatttgct tatgaagagt attaaaaaaa gattggctat aataaatccg actttttcag
    33721 agtatagaag aagtgctaag ttaaataatt tagatattat agatttagag ttagaaagta
    33781 actttaaact taatatagat ataataaaag aaaatataga aagatttgat agtttattta
    33841 tatgtaatcc taataatcct agtggtaatg tccaagattt aaaggaactt gttaacttgc
    33901 taggtaaaca taataaggta ctaataattg atgaaacttt catggaattt gttgaagatg
    33961 aaagtaaaca tagtttggtt aaatatatag aatcaaataa aaatatattc ataattaaag
    34021 ctgtgactaa attttttggt atgcctggtt taagattagg atatggtctt acaagtaatg
    34081 ctgaaattat gaacaaaatt tatgaataca aagagccatg gactattaat tcttttgcag
    34141 atatactatc caattttatt tttgaagata gagagtatat taaagatagt aaagaatatt
    34201 acataaaaga aagaagctat atgttacaag agttgagaaa cataagaaat ataaaggttt
    34261 atgatacaga tgccaatttt attttgataa gaacatataa aaaaacagct aaagaattga
    34321 aaaaagattt gttcaagcaa ggaaacatat tagttagaga tgcgtctaat tttataggat
    34381 tagatgatag ttttataaga gtggctataa aatctcataa agataacaaa attcttatag
    34441 aaaatatgaa aaatttattg ggtgattaaa atgaaatctt atggaatatg cccagcatct
    34501 tgtggagaat ttgtacaagg tataattgat gatgaagaat atttatgttc atatgcaata
    34561 gatatgtatt ctaaagtgta tattgaagaa aaattagtag atataaattt agggaggtat
    34621 aagtcaaggt tagcaattga aaaggtattt gaaaaattta atttacctaa aaaagatact
    34681 aaaaatattt ctttaaatat aaatagtaaa atacctgttg gaaaaggaat ggcaagttca
    34741 actgctgata taggagctac cataaaggca acattatcat tgatagataa agatttaagt
    34801 agtgaagaga tttctaaatt ggccgcagaa atagaaccaa cagattctat atttatagat
    34861 aaaaacagta tatttaatcc tctaaatggg actgttataa aatacctagg aaatctgacc
    34921 aatgctaaag ttataatact tgaaccaaac aaagtactag atactatgaa aattaggttg
    34981 aggcaagatt atagtagatt aaaaacagaa aataaagaag tcataaaaaa atcatttgct
    35041 cttttagaag aaggattgaa aaaaaataat ttatctctag ttggagaggc ttgtacttta
    35101 agtagcttag caaatgagaa tatagacaaa aaagagtatt taaatgaaat aataaaaata
    35161 tcaaaaaaat atggagcata tggagtaaat attgctcata gtggaactgt agtaggtatt
    35221 ttaattgata agttcatgaa tgataaaaat atgatagatg ccttgtgtga atctaacata
    35281 aattctgtct atagtaaaat ttatacacta aacataatta atggaggaat taagggggaa
    35341 atagaatgga atacataaag aatcctatga aaatagaaga aaaaagtttt gaattgatac
    35401 aagaaataat agacgaaatt agaccagatt ataaatttaa gaataatatc gaagaaaaaa
    35461 taataaagag agctatacat actactgcag attttgatta cttagatata ttaaaaatat
    35521 cagaagaagc agttgcgagt ataatagatg cattaagaaa taatgcaagt atttatactg
    35581 atactaatat ggctcttagt ggtataaata aaagaaagtt agaatcatta ggatgtaagt
    35641 ataaatgttt agtggcagat gatgaaactg taaaattagc taaagaaaga ggaattactc
    35701 gttctatggc agcggtagaa attgcagcta aagaagaagg aagaaaaata ttcgttttag
    35761 gcaatgcacc aacagcttta tataaagtta tggagatgaa atcagaaggt agattagatt
    35821 tagatgcagt tataggagtt cctgtaggtt ttgtaggagc agaagaatca aaagatgaag
    35881 ttgaaaaaac agatatacca tatataattt ctaaaggtag aaaaggtggc agtaatttag
    35941 cagcagcaat agtaaatgct atattatata ctatgtaggt acatatatgg aagaatatgt
    36001 atatatagat ggtaaaaaat atagaagagg atatactaca ggttcatgtg caacaggtgc
    36061 atctaaggct gctgtatata tgttgattac aaaaaataga atcaatacta taaatataga
    36121 cactcctaaa ggaataccat tatcactaaa agttgataat ataaatattt ctgatacatt
    36181 tgtggaatgt tcaataaaaa aagatggtgg agatgatata gatgcaactc ataccatgga
    36241 tatttatgca agagctgaaa ttgtatctaa aaatgataag aacaaagagt atcttactct
    36301 aaaggatata gatggtttaa gtaacaatag tgaatcccaa agtgaactct ataagtttat
    36361 tcgggtttat ggtggaattg gtattggtgt ggttacgaaa aaagggctta gtgtagatgt
    36421 tggtaagcct gctataaatc cgactcctct taaaatgata aatcatgaaa taagaaatct
    36481 tataggagat aattttgaat ctatacttgg aaataataaa gttttaaaaa taacaatctt
    36541 tgctccacaa ggagaagctg tagcaaaaaa aacatttaat ccaagattag gtatagttgg
    36601 agggatatct ataataggta caactgggat tgttgaaccc atgagtgatg agggctggaa
    36661 gaagtctcta tctatagaac ttcaaatgaa aaaagaacaa ggtttagata aaataatatt
    36721 ggttccagga aatcatggag aacagtttat aagagaaaaa ttaaatttag atattaagta
    36781 tgtagttagg acaagtaatt tcataggtta tatgattaaa gaagctcaaa gaataggata
    36841 taaaaaaatc ttaatggcag gtcatatagg taaatttata aaaatatctg ctggtatttt
    36901 taatactcat agtaaggtgg cagatgctag aagtgagata ttagtggcaa acttagcttt
    36961 aatgggagct acatacgaat ttttaaataa aataaatcaa tgcctaacta ctgaagaagc
    37021 tgtagaatta ataaataata gtgagtatag acaagtgtat aatatattga gtaataaatg
    37081 cagggagaga gtaaagcaat acttgaatga agattcagat gatattgatg tagaagtaat
    37141 catattttca atggataagt ctttattagg taaatcggat aatacagatg atttagtgga
    37201 ggtctttata tgataaacat aataggtcta ggacctggta acttagacta tataacaaaa
    37261 aaaggtgaaa atttaatttc cacttctgat gtattaattg gtggtaagag aaatttagag
    37321 tctataaaaa actttgaagg agaaaaaata gtcttagatt ctaatttaag agaaattata
    37381 gagtacataa ataacaataa agaaaagcaa atatcaataa tagcatctgg tgacccatta
    37441 atatatggga taggtagata tatatctaaa aatattgata ataagatgct aaatatggtt
    37501 tctgggatta gttcattaca gtacatattt tcaaaaatat atgttgatat gaatgatgta
    37561 tatattacaa gtagccatgg taaagtacca gattttgatt acatattatc acataaaaaa
    37621 gtatgtatgg ttacagactc taagatagga ccaaaacaaa taagtagaga agtaatagat
    37681 agaaatctaa ataaaattat tgttgtagga gaaaatttgt catatgacaa tgaaaaaata
    37741 acaatagcaa aaccagaaga gataataaga atggacaatt ttgatatgaa tgttgtggtg
    37801 attttagatg aggaatagtg aatttgtaac aggtaaagtt ccaattacta aggaagaagt
    37861 tagagcaata tcaataagca agttaaactt agtaaatgct aaaaacttca tagacatagg
    37921 agctggaaca ggaagtgtaa gtgttgaagc tgcatataat tatcctaatt taaaggttat
    37981 atctatagag aaaaatgatg atgctataga gcttatagag aaaaatataa ggaagtttaa
    38041 tttaaaaaat atagaagtga taaaaggata tgctccaatt aacctaggat taaatactaa
    38101 agttgagtct atttttcttg ggggaacagg aaataatctg gaagaaataa tagaatggtc
    38161 taaaaaaaat ttggttgtag gaggaagact tgtagctaat tttataataa tagatacatt
    38221 taatcaaact ttaaaactgc ttaaaaagca tggatttaaa gaaatagatg tatgtgtttt
    38281 gaatgtttct aaactagaga aactaggaaa aggtgaatac tttaaaccac taaatcctat
    38341 atatataata tcttgtgaaa aaggggaaaa tgatgatgaa taaagtacat tttgtaggag
    38401 caggacctgg agataaagag ttgataacat taaaaggata taaattatta agtgatgcag
    38461 atgtagtgat atatgcaggt tcacttgtta atcctgaact tttggaatac tgtaaagaag
    38521 attgccaaat acataatagt gcacatatgg atttacaaga gataatagat gttatgagag
    38581 aaggtataga aaataataag tctgttgtaa gactgcaaac aggtgatttt tctatatatg
    38641 gttcaattag agagcaagtg gaagatttga ataaattgaa tatagattat gactgtactc
    38701 caggagtaag ttctttttta ggtgcagcat catctttagg agttgaatat acagttccag
    38761 aaatttctca aagtgtaatt ataacaagaa tggaaggtag aactccagtt ccagaaaaag
    38821 aatcaataca atcatatgct aagcatcaaa catctatggt aatattttta tctgttcaag
    38881 aaattgagaa agttgtgtct agattgctgg aaggtggata tccaaaagat actcctatag
    38941 cagttatata caaagcaact tgggcagatg aaaaaatagt taaaggaact ttaagtgata
    39001 tagctgtaaa agttaaagaa aacaacataa ataagaccgc tttaataatg gttggaagat
    39061 ttttaggaga agagtataat aattcaaagt tatatgataa ggatttcaaa catgaatata
    39121 gagggtaata ttgctataat ctgtatcaca gaaaatggga aaaaattagc tgtaaagata
    39181 aattctctag taaataactg tgttatatat caaataaaaa ataaaaaaag caatttaaat
    39241 gttgaagaaa cgtgtgtaca tacagttcaa aagaaattaa gtgattttgt aggagaaata
    39301 tttaataaat ttgattacat agtatttata atggctacag gtatagttgt acgaagtata
    39361 gcaccataca taataagtaa gtttagcgac cctgcagttt tggtaatgga tgaaaaaggc
    39421 gaaaacgtaa taagtttatt gagtgggcat attggagggg caaacagttt gacacagtac
    39481 ataagtagtc ttattgatgc taatccagta ataacaacag ctactgatgt aaatcaaaaa
    39541 gcttctttag atatgatagc aaaaaaacta aatgcatata ttgatacttt tagagaaagt
    39601 gtaaaagaag taaattctat gctagtgaac aataaatgtg ttggattata tattgatgaa
    39661 ggatatcaag ttgatactag aggatttaaa cttctagaaa gtaaggatga gttaagagat
    39721 agtttttcaa atatatatat agaaaaatta gttgtagtaa gcaataaaat aaattgtatt
    39781 gaagatattg agaatttaaa cttagataat aaatttgaac tttactatag tactgaaaaa
    39841 ataataaaag taattccaaa agatatagta ataggaattg gatgtagaag aaacacagat
    39901 agtgagttga tgtataattc acttaaagag tttcttaata aacaaaatat agatattaga
    39961 gcaataaata cagttggaag tatagaactg aaaaaagacg aaaaagcaat aattgaatta
    40021 tcaaaaacac taaaagtgcc atttaagaca ttttcaatag atgaaatatc taaggttgat
    40081 aatctttttg ataaatctga ttttgttaaa aaaaatgtgg gcgtgtattc agtagcagag
    40141 cctgtagcat atcttttgag caaaggtaat ttgttagttg aaaaacataa atataaagga
    40201 ataacttttt cagtggggag aataaaaata tgatatacgt tgtaggaatt ggaccaggaa
    40261 gtaaagatac aatgacttta gaagcaataa aagcaataga agattctgag gctattgttg
    40321 gatataagac ttatataaaa ttaatagaag aatttataca agataaagaa ataatccaaa
    40381 atggtatgag gcaagaagtt gatagatgta aacaagcagt agcggaagca aaaaaaggaa
    40441 aaaaagtagc agttgtaagc agtggtgatg ctggaatata tgggatggca ggtcttattt
    40501 tagaattaat atctaaagaa gatgaagata taaaagtaaa ggtaattcca ggtgttactg
    40561 caagtattgg tgcagcagct atactcggag ctcctattat gcatgatttt tgtcacataa
    40621 gcttgagtga tttaatgact ccgtgggaag taattgaaaa acgattgaga ttagctgctg
    40681 aagctgattt tgtaatttgt ttgtataatc caagaagtaa gggaagaagt gaacatctaa
    40741 gcaaagcatt taaaataatg ggtgagttta aagatggaga tacacctgtt ggagtggtaa
    40801 aagatgtagg tagagaaaaa gaagaaaagt ttgtatgtac ttttgaaaat atggactttg
    40861 aaaaagtcga tatgactaca atggttataa taggaaataa atctacttat attgatggtg
    40921 aattgatgat aactccaaga ggttatacag tatgatttta attttaggag gaacttcaga
    40981 tagtttatct atatgtgata agataaatga actgaaaaac caaccatata ttctttcagt
    41041 gactacagat tatggaaggg aacttgcaca acaacatgca aataatgtaa ttttaggtaa
    41101 gcttggaaaa gaagatatgt taaaatttat aaaggataat aatatagtaa aaataataga
    41161 tgccactcat ccttatgcag ttgaagtatc caaaaatgct attacttgtg caaaattatc
    41221 gaatatagat tatataagat ttgagagaaa atctttaatt gatgaaatag attatgataa
    41281 taaatttata gtgaatacaa tcgaagaagc ctgtgaaata gcaaataaaa tagggacaaa
    41341 tatattcata ggaacaggaa gtaaaaattt gaacaggttt atagaggaaa taagtgataa
    41401 aaatttagtt gtgagagttc ttccaactag tgaagtcata ttaagttgtg aaaaattagg
    41461 tttaagtgca gacaatataa tagctatgaa aggtccgttt agccagtata taaatgaaga
    41521 aacatataaa cattacaata tagatttagt tataacaaaa gaaagtggaa tagctggtgg
    41581 attcttagag aaggtaaatg catgtaaaac tttcaatata ccagtaataa ttataaaaag
    41641 agaatcaatt aactacgaaa atgtatttaa tgacataaat aaaattaaag acatacttgt
    41701 gtaattttta ggaggagaat atatgaagaa agccttatta gtagtgagtt ttggtacaag
    41761 ctatcatgaa acgagagaaa agacaataga tatatgtgaa gataaaataa aaaattcatt
    41821 aaaaagttat gattttttta gagcatatac atcaaatatg ataataaata aaataaaaaa
    41881 aagagatgga atagaaatag ataatccaat acaagcttta gataaaatat atgagcaagg
    41941 atatgatgaa gtaataatcc aaacacttca tattatatgt ggcgaagaat ttaacaaatt
    42001 aaaagagcaa gtagatggct attcttccaa attcaaaaag attgtattag gaagaccttt
    42061 acttacacat attgaagatt atcaggaagc tgtagaggca attaagcatc aaattcctca
    42121 tatggaaagt gatgaagcag tagtatttat gggacacgga acacttcatg aatcacattc
    42181 agcgtatcct gcattagaat acatgcttag agacaatgga gtaaatgctt atgttggtac
    42241 agttgaaggg tatccagaaa tagaacatgt aataagaaga ctgaaagaag gaaatataaa
    42301 aactgtaaat ttgatgccat ttatgttagt tgcaggagac catgctataa atgatatggc
    42361 aggagatgaa gaagattctt ggaaaacaat attagaagaa gatggattta atgtaagagt
    42421 tcatttaaaa gggctaggag aaaattccta tatacaagat aaatttgttc gtcatgcttt
    42481 aaaatgtgca gagaatgcta agttttatgg tataggagcg ggtccgggag atggttcttt
    42541 acttacaata aaagcagtta atactttaaa gaaattggat attctataca ctccagaatc
    42601 aaaaaagggt ggagatagtc tagctttatc aatagttagt gaatacttac ctgaatcagt
    42661 ggaaataaaa tctaggcatt ttccaatgag ttttgatggg aatgaaaaaa gtttagcttg
    42721 ggataatgtg gcacaagaaa ttatatcaga tgttaatgat ggtaaaaatg taggatttgt
    42781 tacattagga gaccctatga tttatagcac gtatgtatat ataatgaaaa gaatattagg
    42841 agaggtagaa gtcgaaacta taccaggaat ttcatcattt tcgaatatag catctaatca
    42901 aaattttcca ttagttatgg acaaagaagc attagtggta attccttgta ctatggaaga
    42961 tgataaaata gagtatgctt tgcaaaatta taactcaata gtattgatga aggtatataa
    43021 aaatttcaaa gaaataataa aaaaattaga aaagtatgat ttaatagaac atgctatttt
    43081 agtaagtaat tcttcccaag aaagcgaaaa tgtatttaca gatttaaaag aagcgcacct
    43141 tgaagataag atatcttatt tttctactat actagtaaat aaagataata aaataaaata
    43201 ggtgaaagca atatttaaag gagatttaat atgaatatag tggttggaac taggggtagt
    43261 aatctagcac ttatacaaac agaatgggta ataaatgaat tgaaaaagaa gtatccagaa
    43321 attagttttg agattaaaat cataaagaca aaaggagatt taattcaaaa tgtatcactt
    43381 gataaaatag gtgataaagg attgtttgtt aaagaaatag agcaacaatt attgtatgga
    43441 aaaatagaca tagcagtaca tagtatgaaa gatatgcctt cttatttagc agatggatta
    43501 aaatttgctc atacgcctaa gagagaagac ccaagagatg ttctaatact tagggaagga
    43561 tataaaaatt tagatgattt gcctcgtgga gcagtaatag gaacaggaag caaaagaaga
    43621 aagtttcaat tattgaaaca aagacctgat ttaaatatag tacaagttag aggtaatgtg
    43681 gaaactagga taagaaaaat aaaagatgaa aatatgcatg gtattgtgtt ggcagcatca
    43741 ggaataatta gagctaattt acaagataaa ataagttctt atttacctgt tgatgttgtt
    43801 attccagcac ctgcccaagg agccttagct atagaaatta gaagtaacga tagtaagata
    43861 gagggaatag taaattcact gaaagatgag aatacagaaa ttcaaatatt ggctgaaaga
    43921 ggttttttag atggtgtaaa tggaagttgt catattccaa tggcagctta ttgtgaaata
    43981 atccaagata aaatacatct tactggatta tatggagatt cagaaggaaa aaaagtagta
    44041 atcaagtcta tagatggaga tatttcttct cctagagagt taggtttaaa gttagctaaa
    44101 cttgtgttga aggagtatga gaattatgaa gggtaaggtt tacttagtag gggcaggacc
    44161 aggtgattat aaactcatga ctttaaaggg tttagagtgt ataagaaaat cggatgtaat
    44221 agtatatgat agattagcaa atagtaatta tttaagagaa gctaagcctg attgtgaatt
    44281 tatatatgta ggaaaggcat cgagtaatca tattttgcca caagaagata taaatagaat
    44341 tatagcagaa aaagctaaag aaggaaaaat tgtaactcgt cttaaagggg gagaccctta
    44401 tgtatttggt agagggggag aagaagcaga gactttagta gatgaaggta tagaatttga
    44461 agtagttcct ggtataactt ctgcaatagg aggtttatgt tatgcgggta ttcctataac
    44521 acatagagac catgcttcgt catttcatgt aataacaggt catctgaagg gtgatgatag
    44581 tggagaatta aactggaatg ctcttgcaaa taataaaggt actcttgtgt ttttgatggg
    44641 aatctcaaac ctaaataaaa taagtgaaaa cttaatgaaa gaaggtaaag ataagaatac
    44701 acctgttgct cttataagtt gggcaactag atataatcaa agagttgtta catctacact
    44761 tgaaaatgtt tatgaaactg ctattaaaga agaggtaaaa ccgccaactc ttatagtagt
    44821 tggaagtgtt gtagaactta gagaaaagtt gaatttcttt gaaagtaaac ctttatttgg
    44881 aaaaagtatt gctgtaacta ggtctagaaa tcagaatagt gtattagtag aaaaaataat
    44941 ggatttaggt ggaaatccaa tagaaatacc tactattaag gtagaaaaaa tacaaaacaa
    45001 tattaaatta gaaaatgaaa taaaaaatat aaataagtat aattatctaa tactaactag
    45061 taaaaatgcc gtagaaattt tctttgaaaa gatatatgaa atgaattttg atttaagaat
    45121 actatctaat ttaaaggtat gtgctatagg ttctgctaca tcaaacgagt tgaaaaagag
    45181 aggaattata gcagatatag tacctaaaaa atttgtggca gaatcactgt atgaagagtt
    45241 aatgcctatt ttaaataata gtgataatat acttatacct agagcagaaa atgctagaga
    45301 ttatctggtt gacaaactta aggccatttg tgaagtaaca gaagtacata tatatagaac
    45361 agtaatagat gaggataaaa aagatgagat attagatatt ttaaattcta ataatataga
    45421 ttatataaca tttactagtt cctcaacagt aaataatctc gttgaaatta taggaaaaga
    45481 aaatattaaa atattagaaa aaataaaaac attatctata gggcctataa ctacagatac
    45541 aataaatagt ttgggaataa aattgtacaa agaatctgct acatctacta tagattcgtt
    45601 agtagattta attataaaaa attaattttg gaggcttgaa tatgttaaga agaccaagaa
    45661 gactaagagc aagtaaagca atcagaaatt tggtaagaga aactaaagtg aatgtagaag
    45721 atttaatata tccactgttt gtagtagagg gggaaaatat aaaagaagaa attaaatctt
    45781 taccagatgt ctatcattta tcaatagaca tgttagaaga agaaataaaa gaaataagtg
    45841 agttgggaat tgaacatata atgttatttg gtgttccaga tgaaaaagat gagtgtgctt
    45901 cacaagcttt taatgataat ggaatagttc aaaaggcaat tagaaaggta aaagaaataa
    45961 atgcagatat gaatgtaata acagatgtct gtatgtgcca atatacaagt catggacatt
    46021 gtgggatttt aactgatact ggttatgttg ataatgataa atcattagag tatctagcta
    46081 aaatatctgt aagccatgct aaagcaggag ccaatatggt tgctccatct gatatgatgg
    46141 atggaagaat acaagcaata agagaagctt tagatgaaaa tggattagaa aatgttggaa
    46201 ttatggctta tagtgttaag tatgcttcaa attattatgg accttttaga gaagcagctc
    46261 actcagcacc atcttttgga gatagaaaaa catatcaaat ggatccagca aatagtaatg
    46321 aagctataat agagactgaa cttgatatat tagaaggtgc agatatactt atggttaaac
    46381 ctgcattatc atatttagat attataagaa gggttaaaga taattttgat ttacctctag
    46441 cagcttataa tgtaagtgga gaatactcta tgcttaagtt tgctgtgaag gaaggtttat
    46501 taaatgaaga tgtaattctt gaatcaatta catctataaa aagagcagga gcagatataa
    46561 taataactta ttttgctaag gatattgcca agttattaaa aaataaattt tagctagaaa
    46621 aagaatttta aaaagtatag gaattgtaat tagatattat ataatatttt tatttaattt
    46681 gttgaataag aatagatact aatttataat ttctatgctt tatttgtata atcaaataaa
    46741 gtttggaggt ataaaatgct atatcctatt aatttaaaat tagatgactt agatgtagtt
    46801 ataattggtg gaggagaagt tgcatataga aaatgtaaaa actttttaaa tttcaataaa
    46861 agagttacta tagtttctaa acaactgtta aataagtttt atgatttgaa agaatacata
    46921 aatataatag aagatgatta taaagaagtg tatatagaaa aagcctctat agtaattgct
    46981 gcaacaaaca ataaagtatt gaatatggaa ataggattat attgtagagc ggaacataaa
    47041 ttagtaaatg ttgttgataa tgtagaaata tcaaatttta ctgttccatc atatataaaa
    47101 agaggcgatt tacttataag tgtatctact ggaggcaaga gtccttcact gtcgtcaaag
    47161 ataaaaaaag agattgaaga gatatatact gaagattatg aggagtattt aaatgtatta
    47221 ggagatataa gaaaaaaggt tataaaaaaa tacgaagata aaagtaaaag aaaagatgtg
    47281 cttaacatgt tgataacact tgacttagaa gaacttaaga agtttgatat ataaaaaata
    47341 aaataaaaaa tgttaaaaaa gtgttgacg
//
LOCUS       DAFNNB010000012        43959 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0012, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000012 DAFNNB010000000
VERSION     DAFNNB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43959
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0012"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(40..813)
                     /locus_tag="L3M60_000597"
     CDS             complement(40..813)
                     /locus_tag="L3M60_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HBN6225300.1"
                     /translation="MKNVANATHYLNMDTKELFNLYNKNKNVDIRNILIERHLYLARL
                     LAKKYINKGVDFEDIYQVASLALIYAIDRYDVEKGFEFSSFATPTIVGEIKKYFRDKV
                     WTLRVPRRIQELSKKISDAKIKLEQENKKHPKVKDIADYIGVSEEDVLEAMEASYGYQ
                     PMSLDSSSNDDSEDKDITLIDKIGKEEGNFGSIEYEDFINKFIETLNELEVKIFKDRF
                     FFDKTQSSIAKELGISQMTVSRLEKKVVEKLKKEYEKNL"
     gene            complement(813..1199)
                     /locus_tag="L3M60_000598"
     CDS             complement(813..1199)
                     /locus_tag="L3M60_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6225301.1"
                     /translation="MEITTNPDYVSIIRLTASGIANKMGFPIDDIEDIKVAVSEACTN
                     AIKHSKDDMFNIVFNILDNAINIEIQDNGKGYDVSSISTPDLENPKESGLGLFIIKTL
                     MDDVDIESEHNQGTKIKMTKYLGVDI"
     gene            complement(1224..1541)
                     /locus_tag="L3M60_000599"
     CDS             complement(1224..1541)
                     /locus_tag="L3M60_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HBN6225302.1"
                     /translation="MSMNIDSNLDSQNKFWNVCLDGELDVSTADKLKEHLHGLIEKNM
                     LDVKINLKDLDYIDSTGLGAMIGVLKKLKINEKEIYIVNPKSNVRKIFTITGLDKIFK
                     MEG"
     gene            complement(1553..1813)
                     /locus_tag="L3M60_000600"
     CDS             complement(1553..1813)
                     /locus_tag="L3M60_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HBN6225303.1"
                     /translation="MNAWGWQIGAVLVGSSALIVAIYLAKTLNSINKVVEKAYKIVDY
                     NERHIQDIVENASSISKGIDDIVYVINKILSIGNIFKIIKRK"
     gene            complement(1813..2109)
                     /locus_tag="L3M60_000601"
     CDS             complement(1813..2109)
                     /locus_tag="L3M60_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBN6225304.1"
                     /translation="MCNKRSCKKGGFLLLGAILGFIFGMFFAPKKGSELRKETKEKFN
                     DVKENPKEVLHETFNDVKERIINLVDDDNNEEDIKISEEDIVISKSFDDEGDVN"
     gene            complement(2178..4604)
                     /gene="gyrA"
                     /locus_tag="L3M60_000602"
     CDS             complement(2178..4604)
                     /gene="gyrA"
                     /locus_tag="L3M60_000602"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HBN6225305.1"
                     /translation="MEENNKILPIEIAEEMKKSYIDYSMSVIAGRALPDVRDGLKPVH
                     RRILYSMSELNLTPDKPYRKSARIVGDVLGKYHPHGDTAVYYAMVRMAQDFSTRALLV
                     DGHGNFGSVDGDSPAAMRYTEAKMSKLSLELLRDIEKETVDFKPNFDESLKEPSVLPA
                     RYPNLLVNGSNGIAVGMATSIPPHNLAEVIDATVYLIDNPECSVDDLIKFVQGPDFPT
                     AAIIMGKESIAEAYRTGRGKVKVRSRAFIEELPKGKQQIIVTEIPYQVNKAKLVERIA
                     ELVKEKRIEGISDLRDESNRNGMRIVIELKRDANANIVLNNLYKHSQMEDTFSIIMLA
                     LVDGQPRVLNLKQILYHYIKHQEDVVTRRTKFELNKAEARAHILEGLKIALDNIDAVI
                     SLIRASKTGQEAKLGLIEKFNLTEIQAQAILDMRLQRLTGLERDKIEAEYEDLIKKIN
                     RLKEILADERLLLNVIKDEITIIKENYSDERRTEIRHAEGEIDMRDLISDEEIAITLT
                     HFGYIKRLPSDTYKSQKRGGRGISALTTREEDFVRHLVTTTTHSRLLFFTNKGRVFKL
                     NAYEIPEGKRQAKGTAIVNLLQLSADEKIATLIPIDGNDENEYLLLATKKGIVKKTKR
                     EEFKNINKSGLIAIGLRDDDELIGVELTDGKQEVLLVTKEGMSIRFDENDIRYMGRTA
                     MGVKGITLSKEDFVVSMNLCSKGTDVLVVSKNGFGKRTNIEEYRSQIRAGKGIKTYNI
                     SEKTGTIVGADMVNEDDEIMIINSDGVLIRIRVNEISLFGRVTSGVKLMKTNDEVNVV
                     SIAKINIEEE"
     gene            complement(4622..6523)
                     /gene="gyrB"
                     /locus_tag="L3M60_000603"
     CDS             complement(4622..6523)
                     /gene="gyrB"
                     /locus_tag="L3M60_000603"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003226808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBN6225306.1"
                     /translation="MKQEYGASQIQVLEGLEAVRKRPGMYIGSTSPRGLHHLVYEVVD
                     NSIDEALQGYCSDIYVSINEDGSVLVKDNGRGIPVEIHPKTGKSTLETVLTNLHAGGK
                     FGGGGYKVSGGLHGVGVSVVNALSKWMVAEVYLNGKIYKQTYEKGLPTSKLEVVGESH
                     DKGTMIQFMPDETIFDEIEFKYETLEYRLRELSFLNKGIKIVFEDKREGQEKRKEFHY
                     TGGLVEYIKYLNKSRTGIHDDIVYIDKKVDDCFVELAMQYTDGYTENIYSFANNINTH
                     EGGSHLSGFKAALTKTVNDYAKRNKFLKENDVNLLGEDIREGLTAVVSVKLPEPQFEG
                     QTKTKLGNSFMRGIVDSVTVDELGSFLEENPVTARIIVDKALRAQRAREAAKKARELT
                     RRKSVLESTSLPGKLADCAEKDPAKSEIFLVEGNSAGGSAKQGRDRNSQAILPLRGKI
                     LNVEKSRLDRILSSDEIKNMITAYGCGIGEDFDIDKARYHKIIIMTDADVDGAHIRTL
                     LLTFFFRYMRPLIDEGYVYAAQPPLYKVTKQKKEHYVYSDKELNILLDEIGRNGVELQ
                     RYKGLGEMNAEQLWETTMNPETRTLLQVTVEDAAIADEVFSMLMGDKVAPRKEFIEEN
                     ARFVRNLDI"
     gene            complement(6524..7639)
                     /gene="recF"
                     /locus_tag="L3M60_000604"
     CDS             complement(6524..7639)
                     /gene="recF"
                     /locus_tag="L3M60_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBN6225307.1"
                     /translation="MKLKSLQLVNFRNYKKLHLEFNGKVNLLVGKNGQGKTNIVESIY
                     MLSFGKSFRTNKDKEMVRFNSENLYIGGSFSKYNKYSLIELIIGKDKKGIRVNKVPLQ
                     KIQELLGNLNVVIFSPEDLRLVKEGPKERRTFIDKEISQIIPKYYKYLTNYNKTLSQR
                     SRVLKSIHVDEALLDVYDDTLARYGSYIYILRRDFIKKIASISENMHMNLTNGVERLS
                     IRYKNQINITDEDTIDTVYNKFLAKLSSNRPNDIESKTTRYGIHKDDLNIFINDLDAR
                     LFGSQGQQRTASISLKLSEIELIKNEVEEYPVLILDDVFSELDEARQKLLVNNLSNVQ
                     MFITSAEISHKKIFDEKNVTIFNIENGDVISIENGGN"
     gene            complement(7656..7862)
                     /gene="yaaA"
                     /locus_tag="L3M60_000605"
     CDS             complement(7656..7862)
                     /gene="yaaA"
                     /locus_tag="L3M60_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HBN6225308.1"
                     /translation="MTEITIESEYIKLDQFLKLAEIASTGGHAKFLIQEGLVTVNDEI
                     ELRRGKKIKSGDIVEIEGTKIKVL"
     gene            complement(7999..9105)
                     /locus_tag="L3M60_000606"
     CDS             complement(7999..9105)
                     /locus_tag="L3M60_000606"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBN6225309.1"
                     /translation="MKIICNQKILANRIGIAQKAINGKTTIELLKGILISTEEGQLKL
                     TGYDAEIGIETYVQAEIIEKGDVVVDARLFGDIIRKLPDSFVEIETDSENNIYINCVN
                     SRFKIKGYAAKEFPKLPELNEEDLYSIPQEILKNMIKQTVFAISQDQTKPVLMGELLE
                     IVDRNLNLVAIDGYRLAVKSCSVDSLTENIKVIIPGKTLIDVNSLLSGEDNVKVGFNE
                     KNAIFIINDTKIITRLLEGDFIDYKKLLPREHNSRVKLNTKELLNSIERASLLSQSEK
                     NNLIKLSIRDKVMAITSNTEKGNVYEEVEIDLDGDYLDIAFNSRYFIEGLKNIDNEEI
                     FIEFTTNVNPCIIKPTDDVNYIYLLLPVRISSNI"
     gene            complement(9347..10669)
                     /gene="dnaA"
                     /locus_tag="L3M60_000607"
     CDS             complement(9347..10669)
                     /gene="dnaA"
                     /locus_tag="L3M60_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBN6225310.1"
                     /translation="MMDIVSLWDKTLQLIKGDLTSVSFNTFFKNIVPLKIHLNDLILL
                     APSDFNKDILENRYLHLIEDAISQLSLKKYNIKFVLSEKEVADLNSDSTDLNYRVLYP
                     NLNPKYTFDTFVIGNSNRFAHAACVAVAESPAKAYNPLFLYGGVGLGKTHLMHAIGHH
                     IVSQKKDSKVVYVSSEKFTNELINSIKDDKNEEFRNKYRNVDVLLIDDIQFIAGKERT
                     QEEFFHTFNTLHEANKQIIISSDRPPKDIPTLEDRLRSRFEMGLITDIQAPDFETRIA
                     ILRKKAQLERIDVPNEVMSYIAKNIKSNIRELEGALTRVVAYSSLSNRVISFDLATEA
                     LKDIITTSKNEEINVLRIKEKVSSVFNLKMEDFNSKKRTRSIAYPRQIAMYLTRELTD
                     LSLPKIGEEFGGRDHTTVIHAHDKVSKDIEESEEIKTKIDKIISDLKG"
     gene            11325..11462
                     /gene="rpmH"
                     /locus_tag="L3M60_000608"
     CDS             11325..11462
                     /gene="rpmH"
                     /locus_tag="L3M60_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBN6225311.1"
                     /translation="MSKRTYQPKKRQRSKEHGFRKRMKTSNGRNVLKRRRAKGRNRLT
                     H"
     gene            11540..11884
                     /gene="rnpA"
                     /locus_tag="L3M60_000609"
     CDS             11540..11884
                     /gene="rnpA"
                     /locus_tag="L3M60_000609"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBN6225312.1"
                     /translation="MDFNRTKGLKKDSDFRKVYKHGKSFANKYLVIYILKNKSDYSRV
                     GISVSKKVGKAITRNRVRRLIKEVYRLNIDEKIKPGYDIVFIARVSSKDATFKDIDKS
                     IKNLVKRTDISI"
     gene            11916..12167
                     /gene="yidD"
                     /locus_tag="L3M60_000610"
     CDS             11916..12167
                     /gene="yidD"
                     /locus_tag="L3M60_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBN6225313.1"
                     /translation="MNKIIYILKEISRYLSNLCIYLVRFYQKYISPLKGPTCRFYPTC
                     SQYSIEAFKKYGFIKGMYLTIRRILKCHPFHPGGYDPLK"
     gene            12199..12906
                     /locus_tag="L3M60_000611"
     CDS             12199..12906
                     /locus_tag="L3M60_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBN6225314.1"
                     /translation="MNIIGNFLGLILKVIFEFVNHYGISIILFTILVKIILLPLTIKQ
                     TKSTKAMQDIQPRIKEIQEKYKNKPEKQNEEIVKLYGEAKINPLSGCLPLLIQFPILI
                     GLFSVLREPVAHGVFANKAAFLAADNGFLWIKSLTSPDYILAVFSGASAYVMQKVMTP
                     KDQLQGSMKVMTYVMAGMSFYWGFIFPAGLTLYWTVSNLFSIAQYYLIMNPLKAKLAA
                     NSKEEKVNEKKPKINKK"
     gene            12875..13504
                     /locus_tag="L3M60_000612"
     CDS             12875..13504
                     /locus_tag="L3M60_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HBN6225315.1"
                     /translation="MRKSLKLIKSKSKEEAISKAIAELNLKAEDIEVEILENPSKGFL
                     GFIGAKDGTYEIFVIEREVDVAKNFIEVMLKNANVDAKVNVSQKDNLIMVDIEGKEAA
                     SLIGRRGETLDSIQFLTGLALNKINKDSHTRVLVDIENYRSKREESLIRYANKVAREV
                     AKTRKIKKLDYMNPYERRIIHSALQNDKYVITYSEGTDPYRRLVIECKR"
     gene            13616..14995
                     /gene="mnmE"
                     /locus_tag="L3M60_000613"
     CDS             13616..14995
                     /gene="mnmE"
                     /locus_tag="L3M60_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBN6225316.1"
                     /translation="MFIDDTIAAIATAPGEGGIGILRISGERALKVAEEIFKSMSGKS
                     IEEYNKRTLIYGNIVDKENIIDEVLLAYMKGPNSYTGEDVIEINCHGGFISVKKILEL
                     ILSKDVRLAEAGEFTKRAFLNGRIDLSQAEAVIDVIKAKTDIAHEVAQNQLEGSLSKK
                     IRELRDKVTEILAHVEVAIDYPEEDIEHITYQTLKDKTDELKKEIKKLYDTAESGKIL
                     REGLKTVIVGKPNVGKSSLLNSILGENRAIVTDIPGTTRDVIEEFVNIKGIPLKIVDT
                     AGIRDTDDIVEKIGVERSKESFTSADLIVMVLDASRKLSEEDIEILEKLKDKQTIVLL
                     NKNDLKQEIEEEKILKYVENNSIIKISALQQEGIEELQDKIESMVYKGSIKNNSSLVV
                     TNSRHKDALSKAYKSATDALIALEQSMPFDFVEVDLKNIWDYLGYINGDTVTEDLLDN
                     IFHNFCIGK"
     gene            15093..16985
                     /gene="mnmG"
                     /locus_tag="L3M60_000614"
     CDS             15093..16985
                     /gene="mnmG"
                     /locus_tag="L3M60_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBN6225317.1"
                     /translation="MKFEAGKYDVIVVGAGHAGCEAALATARMGYKTLIITMSLDSIA
                     LMPCNPSIGGTGKGQLVKEIDALGGQMGLNIDKTYIQSRMLNTAKGPAVHSLRAQADK
                     FKYHEEMKKTLEDEPNLDIAMDEVVEILHEGNVVIGVGTKLGCSFKSKAVILATGVYL
                     NSKIYMGEVAFYEGPNALGYAKYLTDSLVELGLRMRRFKTGTPARVHRDSIDFSVMSL
                     QEGDEKVTPFSFMNENIEKEQEPCYLTRTTEETQKVILDNLKRSAMYSGVIESTGPRY
                     CPSIEDKVVRFSDKTSHQLFIEPEGLNTKEMYIQGISTSLPFEVQLDMYKTIKGLENC
                     KIMRPAYAIEYDCVDPTQLKISLEIKGVENLFSAGQFNGTSGYEEAAAQGLMAGINAV
                     RKIEGKEPFVLDRSEAYIGVLLDDLVTKGTNEPYRMMTSRAEYRLYLRQDNADMRLTQ
                     KGYDIGLVKKDRYERFLNKKAAVEKEFERLKNERVTPKEVNSLLEEKGATPIKVGISL
                     YEFLKRPEVTYELLEELGKGAGEDVSREVKEQCVIITKYEGYIEKQLKQIDQFKKLEN
                     KKLDEKINYSSIEGLRLEARQKLDDIKPISIGQASRISGVSPADISVLLIYLEQVRRT
                     RGGKGE"
     gene            16978..17697
                     /gene="rsmG"
                     /locus_tag="L3M60_000615"
     CDS             16978..17697
                     /gene="rsmG"
                     /locus_tag="L3M60_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBN6225318.1"
                     /translation="MNNMELLKNGIEGFNIDTNDSMLEKFKIYREILIDWNKKMNLTG
                     IEDEKEVYIKHFLDSISAVKNGYIKNGMSIIDVGTGAGFPGIPLKICLDRLELTLLDS
                     LNKRINFLEEVSRVLELDNIKFIHGRAEDFGKNENYREKYDVATARAVAGLPILMEFC
                     VPFVKVGGYFICLKGPNANLELEESQKAIDVLGLEYIEKIDVELPEIDLNHNILVFKK
                     VMETPVKYPRKAGKPAKNPIK"
     gene            17860..18645
                     /gene="noc"
                     /locus_tag="L3M60_000616"
     CDS             17860..18645
                     /gene="noc"
                     /locus_tag="L3M60_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HBN6225319.1"
                     /translation="MEDKRVMEIPIEDVVPNPYQPRKIFSQVSLEELSNSIKVYGIIQ
                     PITVRAKDGKYELIAGERRLRAAKLAELKTIPAIINNMNDESSAVLALLENLQREDLN
                     FIEEAIGYENLIKEHAFTQQQLAEKLGKNQSTVANKLRILKLPNDIKMKLIENNLTER
                     HARAFLKLPSEDLMQSVLDKVIKNELTVKKTEKLIQDVLEETKIQEEPDKKQNIKGAM
                     SIRIYINTIKQAFDAISNTGIDAKYNEIDKGDYMEVVVKIPKK"
     gene            18780..19553
                     /locus_tag="L3M60_000617"
     CDS             18780..19553
                     /locus_tag="L3M60_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBN6225320.1"
                     /translation="MGKVIAVFNQKGGVGKTTTNVNLSASLGTLGKKILVLDLDPQGN
                     TTSGYGINKNEVENTIYEIMLDGLHIKEAIISTEFENVDVVPSATELSGAEIELTSKT
                     NREYILKNSIKAVIDEYDYIFLDCPPSLGMLTINCLTAVDSVLIPIQCEYYALEGVSQ
                     LMETIKLVKSRLNADIEIQGVVLSMFDGRANLSIQVVEEVKKYFKGSVYTTLIPRNVR
                     LAEAPSHGKPVIYYDKRCRGSVAYLELAEEFIDLEEEEW"
     gene            19555..20418
                     /locus_tag="L3M60_000618"
     CDS             19555..20418
                     /locus_tag="L3M60_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBN6225321.1"
                     /translation="MENKSKRSNRLGRGLSALIPEIKGETPEKEIVNIDIDKIYPNEV
                     QPRKQFDEEKIKVLADSIKNYGVLQPIVVKMDENNKYMIIAGERRFRASKLANKNQIP
                     AIIKDIDMKDIMEIALIENLQREDLNSIEEALAYKSLIEHYNVTQEEISEAVGKSRPH
                     ITNTLRLLNLGQDVIEMIDSGRITAGHGKALLRIADKDLQLQIAKKIEEEELSVREVE
                     NIAKKISENKQEEPKKSKPKDVFILDVEDKLRNIFGTKVNISKGKKKGKIEIEYYNDD
                     DLNSIVSMLLE"
     gene            20451..21593
                     /locus_tag="L3M60_000619"
     CDS             20451..21593
                     /locus_tag="L3M60_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBN6225322.1"
                     /translation="MIYLDNAATTYPKPERVYNAVLDCMKNYCANPGRAGHKLAMRAA
                     REIYDTRENIAKLFNVSNPMNIVFTSNATDSLNLAIKGVLQEGDHVITTSMEHNSVIR
                     PIKSLEKKGIENTVVKCDYEGFLNYEDLEKAIKSNTKLIVTTHASNVCGTLIDIKKVG
                     EIAKKHNILFLVDASQTAGVYNIDVNECNIDMLAMPGHKCLFGPQGTGILYVREGLNL
                     NILKEGGTGSKSEEIVQPELFPDKYESGTHNTPGIAGLNQGILFIFERGINNIRQHEE
                     ELCQYMIDKLEEVPDIKIYGPKDSKKRAAVIALNIGNMDSGEVTFLLDSDYNIATRSG
                     IHCSPLAHTTLGTLKQGAVRFSIGYFNTKDEIDKAVEALKKISKNK"
     gene            complement(21729..22304)
                     /locus_tag="L3M60_000620"
     CDS             complement(21729..22304)
                     /locus_tag="L3M60_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HBN6225323.1"
                     /translation="MKNKKIMLVLSILSISIFAVGCTNAQNGNDTSKKETKSNTNVEQ
                     PKEENSTEKEVPNNKTKPTPKEETKTQSATIYSFDVDKTDLIENKVDLSKIDENTLFE
                     ELQKLKVVPESAKLNSFTTKDIDGVKTGILDVSSDFTKSNLGSDAEALMLDSVARTYI
                     KNMNVEQIKITVDGSNYESGHIVLEEGDYLK"
     gene            22564..23856
                     /locus_tag="L3M60_000621"
     CDS             22564..23856
                     /locus_tag="L3M60_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBN6225324.1"
                     /translation="MEILSLGEKIKKLRKEKNMTLKELAGDRITAAQISHIERDKSHT
                     SYELLEYLGDKLDVSVEYLLETKEMQSKKITDNLILQSEIYIKENELNKAEDQIKEVL
                     DICKRYDLMENYGRCNFLMANINLKKSNYNDAIENFEKALYFFIKNSDSENILKCYLN
                     IGKIYMQEDFYKGAISHFNFAEEILSENKFEDVNIHKELYSKMAYCYIKLDNPNMSLK
                     YIEKINEIDNRNDRKEDVDILVLKANNMLEIGKYEESRDYFKKALKILESEDNKTGLA
                     NVYLTICDVYRKIGETDRVLEYSQKVYDIKKNDEDEYMMSGLFKIIEAYIDKEDYDLA
                     RKYCKIALASSIKNKNKFNEYKALRFYANMYKNQNEITLAIEYLIKCIKIVSDLGNNR
                     ILANLYIDLGQLYSNISKEKELEYYQKGVFMYKNLEIM"
     gene            23961..24563
                     /gene="yedF"
                     /locus_tag="L3M60_000622"
     CDS             23961..24563
                     /gene="yedF"
                     /locus_tag="L3M60_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBN6225325.1"
                     /translation="MSIKIDARGLACPKPVINTKRELDKLEEGVVVTIVDNETAKENV
                     LKLAKSLSCEANIVDEKENFISIEIRKGKNILDKQNIVDNKESELDNTCIFISSDKMG
                     LGNDELGKVLIKGFIYTLTESKPYPKYVLLVNGGVKLSAENEETIENLKILENMGVEV
                     LSCGTCLDYYNLKDKLQVGTVTNMYTIVETLKNASNTISI"
     gene            24636..24899
                     /locus_tag="L3M60_000623"
     CDS             24636..24899
                     /locus_tag="L3M60_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HBN6225326.1"
                     /translation="MNQMYIVSFNSTHHAIRSEKLFGENSLKVMALPTPREITASCGI
                     SIKFSFEDMEKIKTILIENNVDIKGIYCISKLENGSKEVEKLD"
     gene            24914..25099
                     /locus_tag="L3M60_000624"
     CDS             24914..25099
                     /locus_tag="L3M60_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HBN6225327.1"
                     /translation="MPMDLKIGDIVELKKQHACGCKEFEIIRTGMDIKIKCTKCSRLI
                     MLDRETLEKRVKKLIKK"
     gene            25202..26398
                     /locus_tag="L3M60_000625"
     CDS             25202..26398
                     /locus_tag="L3M60_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN6225328.1"
                     /translation="MAVKYAKRMQGLQGSEIRELLKLTQQPQIISFAGGMPAPELFPV
                     EEMKKVSVAVLEENGRSAMQYTTTEGYEPLREKIAARMNDKNKTNVNKDDILVTSGSQ
                     QGLDFAGKVFIDEGDVILCESPSYIGAINAFKSYQPKFIDVPTDADGMIMEELEKILE
                     TTDRIKMIYVIPDFQNPTGRTWPLERRKKFMEIVNKFEIPVIEDNPYGDLRFEGETLP
                     SLKSMDTKGLVIFLGTFSKIFCPGYRLGWTCASPEILSKFNFAKQGADLQASTISQME
                     VSKFMDMYDLDAHVDKIKAVYVKRRDVMLKTMEEEFPEGLVFTHPEGGLFTWVELPSN
                     LNAKELMPKCLDKNVAYVAGGGFFPNGGRENTFRLNYSNMPEEKIIEGIKNIAAVLKE
                     AMGVEA"
     gene            26612..26890
                     /locus_tag="L3M60_000626"
     CDS             26612..26890
                     /locus_tag="L3M60_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBN6225329.1"
                     /translation="MRNYELVYVVKPNSDEEVREAILNKVKEVVATDGEIVKVDTWGT
                     KKLAYPIAKFTEGFYVLVNFKSAVDVPKEIDRNLKINENVIRHMIVVA"
     gene            26927..27361
                     /gene="ssb"
                     /locus_tag="L3M60_000627"
     CDS             26927..27361
                     /gene="ssb"
                     /locus_tag="L3M60_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN6225330.1"
                     /translation="MNQVVLVGRLTRDPELRYIPGTGTPVASFTIAIDRDYVKKDGSK
                     ETDFIPVEVMGKSAEFCANYITKGRLVALQGSIRVDNYQTQSGEKRTFTKVSTRSVQA
                     LDSKNKSENSYRESAPAFEPSFEPQGLDPQGFQAIDDDDIPF"
     gene            27380..27607
                     /locus_tag="L3M60_000628"
     CDS             27380..27607
                     /locus_tag="L3M60_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBN6225331.1"
                     /translation="MMNKKRRKKKRVCQFCADKNAKIDYKSTQRLQKYITERGKILPR
                     RISGTCAKHQRELTVAIKRARNIALLPYTLD"
     gene            27750..28070
                     /locus_tag="L3M60_000629"
     CDS             27750..28070
                     /locus_tag="L3M60_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBN6225332.1"
                     /translation="MKIDKGSEITRNIKIIEWMKTEILMSVSDLFNLLFKGVKPLDEA
                     IQDTLANIIMITYLLAKRLGISFRDVDYKVKEKIKIGIDEDHSVERWYGDLSNLKKHM
                     DNRE"
     gene            28078..29034
                     /locus_tag="L3M60_000630"
     CDS             28078..29034
                     /locus_tag="L3M60_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HBN6225333.1"
                     /translation="MNNKIRLSKAMSIIILAIIIALVIAYIPMLGIFSMLAAVPYVII
                     GAITDRKYSLISILVTFCVLILFSDISYALNICIMYSLPGVVIGKMLKRSFEQEDSNK
                     FEPIYGGTIIFVLSMLVYFFVLKTFLNVNLLDEVSKMISEIVNIQKNTFSATELKVFD
                     KMKPDEIVSYFTNMLPMMLFLQGLLSAFVTYYLSVFFIKRITKMNIRFPKFADFYLPG
                     NAILTTFLLYLLVLFIQIIGSRLYTELIMTNLQLVFNLMFVIQGIAVCIYFLKKWIRQ
                     GPNKIMFFSGIILCLFGFMGISFVGMVDSIIDFRKVRSYKST"
     gene            29045..31039
                     /locus_tag="L3M60_000631"
     CDS             29045..31039
                     /locus_tag="L3M60_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HBN6225334.1"
                     /translation="MSNKQTFKLNMPEINLYIIVIGISSIILLYYNLYVGCLFFCIFV
                     YMVFHNWRTTNIRHHEWTEYIQNLSLDIDETTKKAIINLPIPLCILEFDGNISWYNGK
                     FYDMIGQKDLLDKNIEDIVKNLNLRKVLNENKEMYTEINYKEKEYTIIYNVIKNDQEK
                     NPKYLMILYWIDKTEYLKVKQNYDDEKNAMMLIQVDGYDEVLKSAAEDKRALINVEVE
                     KILSALELNSNGALRRTSKDKFFLVMHKKELKKLEAEKFSILDTIRHIDYGNNLPVTI
                     SIGIGIDGDTLNENLKLATGALDLALGRGGDQAVVKTKDKFVFYGGKSKAVEKKTKVK
                     SRLIGHALREVIQQSDQVYIMGHKYPDMDAMGAAVGVYDICKSCNKTANIVLQSVNES
                     IEIFINKINENNYYKKLFIGKEEAIDNCTKNTLVVVVDTHRPNYTECEELLKLSEKVV
                     VIDHHRRGVEFINDAVLLFHEIYVSSTCEMVTELVQYMDEDVTINKLTAEGLLAGISL
                     DTKNFAFKTGVRTFEAASYLRKVGADTIEVKKFFNSDVKDFIIKAEIIQSTKIINNRI
                     CLAYSSTEIDSINVIIAQTADELLNIKEVEASFVLGEKDDTIFISARSLGQINVHVLM
                     EKLGGGGHIDIAGAQLKNVSLKEAYKMVNKIIEEYLEEEE"
     gene            31040..31489
                     /locus_tag="L3M60_000632"
     CDS             31040..31489
                     /locus_tag="L3M60_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBN6225335.1"
                     /translation="MKVILLKDVKGTGKKGEMKEVSDGYARNFLFPKKMAVQADSVAI
                     KELNEKNKSKEIKAQKEYEEAVLLGKQMEEINIEIYSKAGEGGRLFGSITSKEIAEQL
                     KKQKDIDVDKRKILLDEPIRALGSTFVEIKIHQKVTTKIRVDVKEKQ"
     gene            31508..32836
                     /gene="dnaB"
                     /locus_tag="L3M60_000633"
     CDS             31508..32836
                     /gene="dnaB"
                     /locus_tag="L3M60_000633"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBN6225336.1"
                     /translation="MEDMTRIPPHSVESEQSILGSILLDKDAIITVTETIKPDDFYKE
                     AHKIIYECMITLSNKGEPIDLITLTEELRKQGHLNDIGGISYITSLSTIVPTTSNVKY
                     YADIVKEKSVLRKLIKASNEIINLGYSGATKIEDVLEQAEKSIFDISQEKTSDDFKSI
                     NLVLMDAYDMIEKLYTNKSDVTGITTGFKDLNKKINGLQRTDLILIAARPAMGKTAFS
                     LNLVQNAALKGDASVAVFSLEMSKEQLVQRMLSSQSSVELKKIKTGTLNDNDWPRIID
                     AMAVLSDAKIHIDDTPGIKISELRSKCRKLKIEKGLDLVLIDYLQLMEGEGNNESRQQ
                     EISKISRSLKILAKELNCPVVALSQLSRAPEQRADHRPMLSDLRESGAIEQDADIVMF
                     LYRDEYYHADSESKNIGEVIIAKNRHGETGSVELVWLGEVQRFGDKLRDL"
     gene            33041..33349
                     /locus_tag="L3M60_000634"
     CDS             33041..33349
                     /locus_tag="L3M60_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225337.1"
                     /translation="MEKISLKYIYPNIIKVLDEINLFRVIDNNLRESIVVYANNVDNQ
                     YHINMTNTNFGNIINICKLEKLLDVDKFMEKVIKSEKEIIEKEEFSKIEEYMLNIGEY
                     "
     gene            33431..34720
                     /locus_tag="L3M60_000635"
     CDS             33431..34720
                     /locus_tag="L3M60_000635"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBN6225338.1"
                     /translation="MKTVAIVGSQWGDEGKGKVIDYLATQADVVVRGQGGNNAGHTLV
                     VEGKKYALHLIPSGVLNPNTVNIIGNGIVFDPKGFLEELEMFKMDNISTENIKISNRA
                     HVIFPYHKELDVLSEEARGDLKIGTTKKGIGPCYMDKTERSGIRICDLMDKDKFAIKL
                     KAQIDAKNEIVKNIYGKEELFDFETIYNEYLGYAEQIRKYVADTSVIVYDAIRAGKKV
                     LFEGAQGTLLDLDLGTYPFVTSSHPTSGGFAIGAGIGPNMIKDVVGIVKAYTTRVGEG
                     PFVTEQINETGDKIREQGHEFGVTTGRPRRCGWFDAVIVKYAARVNGLTSISFMLLDV
                     LTGFDKIKVCTSYKMGDKIITDFPASLDDLAKCEPVYEELDGWNEDITQIDNFDDLPE
                     NAKKYVAKIEELVGVSVDMVSVGPNRAQTIIRRNIFA"
     gene            35051..35126
                     /locus_tag="L3M60_000636"
     tRNA            35051..35126
                     /locus_tag="L3M60_000636"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35084..35086,aa:Lys,seq:ttt)
     gene            35133..35207
                     /locus_tag="L3M60_000637"
     tRNA            35133..35207
                     /locus_tag="L3M60_000637"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35166..35168,aa:Glu,seq:ttc)
     gene            35212..35287
                     /locus_tag="L3M60_000638"
     tRNA            35212..35287
                     /locus_tag="L3M60_000638"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35245..35247,aa:Val,seq:tac)
     gene            35321..35395
                     /locus_tag="L3M60_000639"
     tRNA            35321..35395
                     /locus_tag="L3M60_000639"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35353..35355,aa:Thr,seq:tgt)
     gene            complement(35777..36769)
                     /locus_tag="L3M60_000640"
     CDS             complement(35777..36769)
                     /locus_tag="L3M60_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6225339.1"
                     /translation="MNEDKIRKILAKYAKRRDDNELLLEHRKNEVYNRIPEIKSLDDE
                     ISKIGLRLAKIVLLNPKSKDEIVKKTKENIESLKLKKEKLLTENNIPLDYLEIKFQCV
                     SCKDKGFLPNGEKCSCLKQEIVNEAYKMSNLDRILSQENFSNFNLNIFSPKKSSDGEI
                     SPRENMLNNLSICENFVHDFKKDNSENLLFYGSTGLGKTYMCNCIAKELLDKGNVVIY
                     QTSFRILDILEDYKFRRDSNNQISEDNYKNLFDCDLLIIDDLGTELNNSFTSGEIFNI
                     VNTRLVAGKKIIISTNLTPSQIGNTYTQRTLSRILDKFRILEFTGDDLRWERFK"
     gene            complement(36778..37836)
                     /locus_tag="L3M60_000641"
     CDS             complement(36778..37836)
                     /locus_tag="L3M60_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HBN6225340.1"
                     /translation="MFFKESNEIDLGEITIPNIFIDIFMPMADGLYVKVYLLGYRQAC
                     DITSNPKFDNSSIAKNLNIPLSDVLSAWKFWEEKKIIKIHDNGEYDNFNYSIEFLDLK
                     NFYIENILSNNSSVKSNTDKVVSTSENPSIRKMFNSINKIVGRYLDPSEKMSIMDIMN
                     KYNMSPDMILCAYEYVKDKTGTSKPVKYIEGIIRNWYDSNLYTPKDVEESFLVRSERY
                     ILYKTIFNELGFSRQPSKSEKELMDTWFDKFNMDIDLIIKACSKSKNISNPSISYING
                     IIKNWNEKNIKNLNDLKQKEEERIVKENINKKQINTTQNNNTYKKTKFHNFNETFTQY
                     TSDELDEIIKKSQKEKFK"
     gene            37961..39424
                     /locus_tag="L3M60_000642"
     CDS             37961..39424
                     /locus_tag="L3M60_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HBN6225341.1"
                     /translation="MVIKFNQKVRRTLSVAILFMVLIIGCFIEKDDSLETYKEKTNSK
                     INQYNMDVIFDDETKRLMCNQNIEYINNTKSNIDKIYLHIYPNAFSKKDFAPFEKSEM
                     TRAYPNGFNEGYIDIKNILNNNSKMEYKIKGDKNDILEIELGKELKPNERMSLDIKYN
                     VKIPNSVGRFGYGENTINVTNWFPIVCVNDERGWNLKSYETIGDPFYSETSDFHVKLL
                     IPNKYKIAHTGKLVNDKHDNKKMLYEIEAKNVRDFAFILSSKFDVNKVDSKGVAINTY
                     NLNKKLSKKATEVAKDSINIFSELFGKYPYKEFSVVASDFFIGGMEYPMLVMIDQSLY
                     NEKNEFLLEYVIAHETAHQWWYSAVGNDEISEPWLDEALTEYSTIVYFEQKYGKDMAN
                     KLIKTMEIQTKSYLSENIFRPANEYKNSTEYSLNVYTKGAIAFNEIRKEVGDKVFFET
                     LNEYYNKYKHKNANGRAFVELWNSKGVDINKIISECK"
     gene            39509..40306
                     /locus_tag="L3M60_000643"
     CDS             39509..40306
                     /locus_tag="L3M60_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6225342.1"
                     /translation="MLKYCSVGSGSSGNCHYIGYKNTNILVDAGLSGKRITTGLKDIG
                     IDADKLKGIFITHEHVDHIKGAGILSRKFDIPVFANIKTWCSMKDKLGDIKDKNMKVF
                     ENDKTYSLGDIIVRPFSIEHDASDPVGYNFYTENDEKMSIATDIGCITQNIKKHLYKS
                     KLVVLESNYDPNMLMMGSYTYALKKRVMSNAGHLSNEDAANFCVELINEGTESILLAH
                     LSKENNFPELAYETSKGVLASNDIVVGQDVKLDVLSRNDVSNVYEMK"
     gene            40319..42421
                     /locus_tag="L3M60_000644"
     CDS             40319..42421
                     /locus_tag="L3M60_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC/EAL
                     domain-containing protein"
                     /protein_id="HBN6225343.1"
                     /translation="MLKRNFENVEDNKILYSIKQGMILAIPAIMTGSTALVILNLPIK
                     VYQEYLSSLFNGEVTNILNFINDSTLGIISLIILLTISYSYGKIYGSKYTVLVPIVAM
                     CSFLVFSHGNELDSYIEIFKTKWLFTSIIVSMTSSVLFVRLTESFVTSVKFHTEGADA
                     DFNMVVSAIVPFIIVVFLFSIFRVIMISLIGSSNFQDIFYNLFSNVFNRMGTNLMSAL
                     LFIFLMHFMWFFGIHGGNVLDTVAKNLFENSMAININLVNNNQLPTEIFTKTFFDTMV
                     LLGGCGFLLCLVIAIFLSEKRINVRKLAKIASIPAVFNINEMLVFGIPIVFNYIMFVP
                     FVITPIILTITSYVAMSTGIVPCTVSAVEWTSPIFLSGYISTGSIRGSLLQLFNLCVG
                     VMIYIPFIKISQNRYTYMFKNNIENLTKLVKRYELTGEQPNFLNHNGKIGSISKMLAS
                     DLKFAMKRGEIEIFYQPQVNYNGDVVGAEGLLRWKHSIGGFIYPPLVIILAKEEGFLD
                     ELGEYIINKACVDLKKSEKLLRNSVKFSVNISPDQLDEPKLPEQIKNIISSNDINPNM
                     LGIEITEQVALSGSQIIIERINAIHNLGIKLIMDDFGMGHSSLIYLQNNNFDIVKLDG
                     SLVKEILTNKRSSEIIMTIVNLSKNLDFDIVVEYVENMDQRDKLYRLGCEIYQGYYYS
                     KAIPFEEFIEYANQEREI"
     gene            42489..42968
                     /gene="rlmH"
                     /locus_tag="L3M60_000645"
     CDS             42489..42968
                     /gene="rlmH"
                     /locus_tag="L3M60_000645"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBN6225344.1"
                     /translation="MNISIVVVGKIKEKYLKLGIDEFKKRLSKYCKLEIIELDDEKAP
                     ENLSSKEMEIIKDKEGKKILGKVKHNSYVIALAIDGKSLSSEELAKTMSDLAVRGNSS
                     LCFIIGGSLGLSDEVLGRADYRLSFSRMTFPHQMMRLILLEQIYRAYRINNGEPYHK"
     gene            43117..43428
                     /locus_tag="L3M60_000646"
     CDS             43117..43428
                     /locus_tag="L3M60_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225345.1"
                     /translation="MIKIMLSCSAGMSTSLLVTKMEIAAKENGIESQIWAIPESTIQN
                     EIEKCDVLLLGPQVRYVLPKAQEIAKPYNIPVEVINMMHYGTVNGEAVLNRAIELNNT
                     K"
     gene            43476..43793
                     /locus_tag="L3M60_000647"
     CDS             43476..43793
                     /locus_tag="L3M60_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBN6225346.1"
                     /translation="MDEKIIEISFNIIGYAGKGKGLAFEAIKEAKNGNIEKAKDLLKE
                     SKEVINKAHKYQTELIQNEASGNKTEISVILIHAQDHLMNGMNFQQLAEEIVDLHLKL
                     QDK"
BASE COUNT        16632 a         5560 c         6960 g        14807 t
ORIGIN      
        1 catctttaaa acagtaggtc aatacttttt tatatttttt tataaatttt tttcatattc
       61 ttttttcagc ttctctacta cctttttctc tagcctagaa acagtcattt gagatatccc
      121 taattcctta gctatactag attgtgtttt atcaaagaaa aatctatctt taaaaatttt
      181 aacttctaat tcatttaaag tttcaataaa cttattaata aaatcttcat actctatact
      241 tccaaagtta ccttcttctt taccaatctt atcaataaga gttatatctt tatcctctga
      301 atcatcatta cttgatgaat ccaaagacat tggttgatat ccataagaag cctccatagc
      361 ctctagaaca tcttcctcac taacgcctat ataatcagct atatctttta cctttggatg
      421 ctttttattt tcttgttcta atttaatctt cgcatcactt atctttttgc ttaattcctg
      481 tatccttctt gggactctaa gtgtccacac cttgtctcta aaatactttt ttatttctcc
      541 tactatagtt ggtgtcgcaa agctggagaa ctcaaaccct ttctcaacat catatctatc
      601 aatagcataa attaatgcca atgaagcaac ttgatatata tcttcaaaat caacaccttt
      661 gtttatatac ttcttagcta acaatcttgc taaatacaga tgtctttcaa taagaatatt
      721 cctaatatct acatttttat ttttattata taaattaaat agctcttttg tatccatatt
      781 taggtaatgt gtagcattag ctacattttt cattaaatat caactcctaa atatttagtc
      841 atttttattt ttgtcccctg attatgttct gattcaatat ctacatcatc cattaaagtt
      901 ttaattataa ataatccaag accactctct tttggattct ctaaatctgg tgtacttatt
      961 gagctaacat catatccttt tccattatct tgaatttcta tattaattgc attatctaaa
     1021 atattaaata ctatattaaa catatcatct ttactatgtt ttattgcatt tgtacatgct
     1081 tcagatactg ctacttttat atcttctata tcatctattg gaaaacccat cttattggca
     1141 attccagagg ctgttaatct tattatacta acataatctg gatttgtagt aatttccatc
     1201 tttatagtct cacaagccaa agtttatccc tccattttaa atattttatc tagacctgtt
     1261 atagtaaaaa tctttcttac attactctta ggatttacta tgtatatctc tttttcattt
     1321 atttttaatt tttttaatac acctatcata gctcctaaac ctgttgagtc tatataatct
     1381 agatctttaa gattaatctt tacatctaac atattctttt ctattaaacc atgtaagtgt
     1441 tcctttagtt tatctgctgt agaaacatca agttctccat caaggcaaac attccaaaat
     1501 ttattttgtg aatctaaatt tgaatcaata ttcatagaca tgtaaaaacc tcctatttcc
     1561 ttttaataat tttaaatata tttcctatac tcaatatttt attaattaca taaactatat
     1621 catctattcc ctttgatatt gatgatgcat tttcaacaat atcttgtata tgtctttcat
     1681 tataatctac aatcttatat gccttttcaa caactttatt tatgctattt agagttttag
     1741 ctaaataaat tgctactatg agagcagagc ttcctactaa cactgctcca atctgccacc
     1801 cccatgcatt cattagttaa catctccctc atcatcaaag cttttactta ttactatatc
     1861 ttcttctgaa atctttatat cttcttcatt attatcatcg tctactaaat ttattattct
     1921 ctctttaaca tcattaaatg tttcatgtag aacttctttt ggattttctt taacatcatt
     1981 aaacttctct tttgtttctt ttcttagttc agaacctttt ttgggtgcaa aaaacatacc
     2041 aaatataaac cctaaaatag ctcctaataa taaaaatcca ccttttttac aacttctttt
     2101 attgcacata acaattactc ctttcaattt taaattttta tctaactaca ttatacaaaa
     2161 agaataggat atttatccta ttcttcttca atatttattt tggcaatcga aactacatta
     2221 acttcatcat tcgtcttcat taatttaaca ccacttgtaa ctcttccaaa cagtgatatt
     2281 tcattgactc ttattctaat aagaactcca tcagaattta taatcattat ctcatcatct
     2341 tcattaacca tatctgcacc tacaattgta ccagtctttt cagagatatt ataagtttta
     2401 attcctttac cagctcttat ttgacttcta tactcttcta tatttgtcct ctttccaaaa
     2461 ccattcttac ttacaactaa cacatctgta cctttactgc aaaggttcat agacacaaca
     2521 aagtcttctt tacttaaagt tatacctttt acacccatgg ctgttctacc catatatctt
     2581 atatcatttt catcaaatct tatagacata ccttctttag ttactaaaag tacttcttgc
     2641 tttccgtctg taagttctac tccaataagc tcatcatcat ctcttaagcc tatagcaata
     2701 agacccgatt tatttatatt tttgaactct tccctcttag tcttcttaac aatacctttt
     2761 ttcgttgcaa gtaataaata ttcattttca tcattaccat caataggtat tagagtagca
     2821 attttctcat ctgctgacaa ttgaagtaaa ttcactatag cagttccttt agcctgtctc
     2881 ttaccttcag gtatttcata tgcatttaat ttaaatactc tacctttatt tgtaaagaat
     2941 aatagtctac tatgagtagt tgtagttact aagtgtctta caaaatcttc ttctctagtt
     3001 gtaagtgctg aaatacctct tccacctctt ttttgactct tataagtatc agatggaagc
     3061 ctttttatat atccaaagtg agtaagagtt attgctattt cttcatcact tataagatct
     3121 ctcatatcta tttcgccttc agcatgtctt atttctgttc ttctctcatc agagtaattt
     3181 tcttttatta ttgtaatttc atcctttatt acattcaaaa gtaatctttc atcagctaaa
     3241 atttctttta atctatttat cttcttgatt aagtcttcat attcagcttc tatcttatct
     3301 ctttctaaac ctgtaagtct ttgaagtctc atatctaata tagcttgtgc ttggatttca
     3361 gttaagttaa atttttctat taaacctaac ttagcttctt gcccagtctt tgaagctctt
     3421 atcaagctta taacagcatc tatattatct aaagcaatct ttaatccttc taaaatgtgt
     3481 gctcttgctt cagctttatt tagttcaaac ttagttcttc ttgtaacaac atcttcttga
     3541 tgtttaatat aatgatataa tatttgtttc agatttaaaa ctcttggctg accatctaca
     3601 agtgcaagca ttattatact aaaagtatct tccatttgag aatgtttata taaattattt
     3661 aatactatat tagcattagc atccctcttt aattctataa caattctcat accatttcta
     3721 ttactttcgt ctcttaggtc tgatatacct tctattctct tttctttaac taactcagct
     3781 attctctcaa ctagtttagc tttatttact tgataaggta tttctgtaac tattatttgc
     3841 tgttttcctt ttggtagttc ttctataaaa gctctagacc taactttaac ttttcctctt
     3901 ccagttctgt atgcttctgc tatactttct ttccccatta taattgcagc tgtagggaaa
     3961 tctggtcctt gaacaaattt tattaaatca tctacactac actctggatt atctatcaaa
     4021 tatacagttg catcaattac ttctgctaag ttatgtggag gtattgaagt tgccatacca
     4081 acagctatac catttgagcc atttactaat aaattaggat atctcgctgg caacactgaa
     4141 ggctctttta atgactcatc aaagtttggt ttaaagtcta cagtttcctt ttcaatatct
     4201 ctcagtagtt ctaatgataa tttactcatt ttagcttctg tataacgcat agcagctggt
     4261 gaatctccat caacagaacc aaagttacca tgaccatcta ctaaaagtgc tctagttgaa
     4321 aaatcttgtg ccattcttac catagcataa taaacagcag tatctccatg aggatggtat
     4381 ttacctaaaa cgtccccaac aatacgagct gacttcctgt atggtttatc tggagttaaa
     4441 tttaactcac tcattgaata tagtattctt ctatgaactg gctttaaacc atctctaaca
     4501 tcaggaagag cacgtccagc tatgacactc attgaataat caatataaga ttttttcatt
     4561 tcttccgcta tttcaatagg gagtattttg ttattttctt ccatgtatac taatcccctc
     4621 tctatatatc taagtttcta acaaatcttg cattttcttc tataaattct tttcttggag
     4681 caaccttatc acccataagc attgaaaata cttcatctgc aattgcagca tcctccacag
     4741 taacttgtaa taaagttctt gtttcaggat tcatagttgt ttcccataat tgctctgcgt
     4801 tcatctctcc aagacctttg tatctttgaa gttcaacacc atttcttcca atttcatcta
     4861 ataagatatt taactcttta tctgaataaa catagtgctc cttcttttgc tttgttactt
     4921 tatataaagg tggttgtgct gcataaacat acccttcatc tataagaggt ctcatatatc
     4981 taaagaagaa tgttaataat aaagttctta tatgggctcc atctacatcg gcatcggtca
     5041 taattataat tttatgatac ctagccttat ctatatcaaa atcttctcca ataccacaac
     5101 cataagctgt tatcatattt tttatttcat ctgaagataa tatcctatct agtcttgact
     5161 tctcaacatt aagtatttta cctcttaatg gaagtatagc ttgactattt ctgtctctac
     5221 cttgcttagc tgaacctccc gccgaatttc cttcgactaa aaatatttca cttttagctg
     5281 gatctttttc tgcacaatct gcaagttttc caggtaaaga tgtactttct agtacacttt
     5341 ttctccttgt taattccctt gctttttttg cagcttctct agctctttgt gctcttaagg
     5401 ctttatcaac tattattctt gctgttactg ggttttcttc taggaaagaa cctaattcat
     5461 caactgttac actatcaact atgcctctca taaagctatt tcctagcttt gttttagttt
     5521 gaccttcaaa ttgaggttct ggtaatttaa ctgatactac tgctgtaaga ccttctctta
     5581 tgtcttcacc aagtagatta acatcatttt ccttcaaaaa cttatttctc ttagcataat
     5641 catttacagt tttagtaaga gctgctttaa atccacttaa atgagaacca ccttcgtgtg
     5701 tatttatatt gtttgcaaaa gaatatatgt tctctgtata accatccgta tattgcatag
     5761 ctagttcgac aaaacaatca tcaacctttt tatctatata aactatgtca tcatgtatac
     5821 cagttctaga tttatttaaa tacttaatat attctaccaa accacctgta tagtgaaatt
     5881 cttttctttt ttcttgtcct tctcttttat cttcaaatac tatttttatt cctttattca
     5941 agaaagaaag ctctctaagt ctatattcca atgtttcata tttaaactct atttcatcaa
     6001 atattgtctc atctggcata aattgaatca ttgttccttt atcgtgagat tctccaacta
     6061 cttcaagctt agatgttggt aatcctttct catatgtttg cttatatatc tttccattta
     6121 agtaaacctc tgctaccatc cattttgaca atgcattaac tacagaaaca ccaactccat
     6181 gaagacctcc agatacttta tatcctccgc ctccaaactt tcctcctgca tgaagattag
     6241 ttaatactgt ttctaaagtt gatttgccag ttttaggatg tatttcaaca ggtatacctc
     6301 ttccgttatc ttttactaaa acactgccat cttcattgat agatacatat atgtctgaac
     6361 aatatccttg taaagcctca tctatactat tatctacaac ttcataaact aagtgatgta
     6421 aacctctagg gcttgtacta ccaatataca tacctggtct ttttctaacg gcttctaatc
     6481 cctctaatac ttgtatctga cttgcaccgt attcttgttt catttaattt cctccattct
     6541 ctatgctaat aacatcgcca ttttcaatat taaatattgt aacatttttt tcatcaaata
     6601 tttttttatg tgaaatctca gcacttgtta taaacatttg aacattgctt aaattattta
     6661 ctaaaagttt ttgtcttgct tcatctaact cactaaaaac atcatctaaa attaacactg
     6721 gatactcttc aacctcattt tttattaatt ctatctctga taactttaat gatattgaag
     6781 cagttctttg ttgaccttgt gaaccaaaaa gtcttgcatc taaatcattt atgaatatat
     6841 ttaagtcatc tttgtgtata ccatatctgg tagtcttaga ttctatatca ttaggcctat
     6901 tcgatgacaa cttggctaaa aatttattat atactgtatc tatagtatct tcgtcagtta
     6961 tatttatttg atttttgtat cgaattgata atctttcaac tccattagtc aaattcatat
     7021 gcatattctc agatatacta gctatctttt ttataaagtc tctacgcaaa atataaatat
     7081 aacttccata ccttgctaat gtatcatcat atacatctaa taaagcttca tcaacatgta
     7141 tacttttaag caccctactc ctttgagaaa gtgttttatt ataattggta agatatttat
     7201 aatatttagg tataatctga ctaatctctt tgtctataaa tgtccttctt tctttaggac
     7261 cctctttaac caacctcaaa tcctcaggag aaaatataac aacatttaag ttgccaagca
     7321 gttcttgtat cttttgcaaa ggaactttat ttactcttat ccccttttta tcttttccaa
     7381 taattagctc tattagacta tatttattat attttgaaaa acttcctcca atatataagt
     7441 tttcactatt aaatctaacc atttctttat ctttgttagt cctaaatgat tttccaaaag
     7501 aaagcatata aatagattct actatatttg tctttccttg accattttta ccaacaagta
     7561 gatttacttt cccattaaat tccagatgca actttttata atttctaaaa ttaacaagtt
     7621 gcaaactttt aagtttcaca aaaagttctc cttttttaca atacttttat tttagttcct
     7681 tctatttcaa ctatatctcc tgattttatc ttttttcctc ttcttaattc tatttcatca
     7741 tttactgtca ctaaaccttc ttgaattaaa aattttgcat gacctcctgt tgatgcaatc
     7801 tctgctaatt taagaaattg atctaacttt atatattctg attctatagt tatttcagtc
     7861 ataatagttc ctccttgtca ctattttaaa caaattccta ttgatacact aaaagtatat
     7921 tataccataa atatgcttat aaaaggagtt gtctaagatt agaaaaattt tctacaaaag
     7981 acaactcctt catgtctgct atatatttga tgatattctt actggaagca gtaagtaaat
     8041 ataattaaca tcatctgttg gtttaattat acaaggattt acatttgtag taaattctat
     8101 aaagatttcc tcattatcta tatttttcaa accttcaata aaatatctag aattgaaagc
     8161 tatgtctaga taatctccat ctaaatctat ttcaacctct tcatacacat ttcctttttc
     8221 tgtattagaa gtaattgcca ttaccttatc tcttatagat aactttataa gattattttt
     8281 ttctgactga gacaataaag acgctctctc tatactattt aaaagctcct tagtgtttaa
     8341 tttaactctg ctgttatgtt ctcttggcaa taacttttta taatcaataa aatctccttc
     8401 aagtaatctt gtgataattt tagtatcatt aattataaaa atagcatttt tttcgttaaa
     8461 cccaacttta acattatctt ctccagaaag caaactattt acatctataa gtgtctttcc
     8521 aggaattatg acttttatat tttcagttaa actatcaact gaacaacttt ttacagctaa
     8581 tctatatcca tcaattgcta ctaaatttaa gtttctatct acaatttcta aaagttctcc
     8641 cattaaaaca ggctttgttt ggtcctgtga gatagcaaat acggtttgtt taatcatatt
     8701 ttttaagatt tcttgaggta tactatataa atcttcttca tttaattctg gtaatttagg
     8761 aaattctttt gctgcatacc ctttaatttt aaatcttgaa tttacacaat ttatgtaaat
     8821 attattttct gaatcagttt ctatctcaac gaaagaatct ggtaattttc taattatatc
     8881 tccaaatagc cttgcatcta caacaacatc tcctttttct atgatttctg cttgtacata
     8941 agtttctatt cctatttcag catcatatcc agtaagtttt aattgacctt cttctgtaga
     9001 tataagtata ccttttaata attctattgt tgtttttcca ttaatagctt tttgagcaat
     9061 tccaattcta tttgctagga ttttttgatt acaaattatt ttcaatgtgg ataacctcct
     9121 ctggcttttt atatataaaa ataagttaaa atcatagtag taatagtagt aggtgttgtt
     9181 gataatgtta ataagtatgt ataacattat aaatacaaag atattaacat gtgtataaga
     9241 tgttaatatc tttgttattt tagacttgat aatttgttaa tactttttgt tcaaaaaaag
     9301 tattaacagg ttattcaaga gatataaaca tacaattgtg tataaattat cccttcaaat
     9361 ctgatataat tttgtctatt ttagtcttaa tttcttcact ttcttctata tccttagaca
     9421 ctttgtcatg agcatgaatt actgttgtat ggtctctacc tccaaactct tcacctattt
     9481 taggaaggga taaatcagta agttcgcgcg ttaaatacat agctatctgt cttggataag
     9541 ctattgaacg tgttcttttt ttagaattaa aatcttccat ctttaaattg aatacagaag
     9601 atactttttc ttttattctg agaacattta tttcttcatt tttagaagtt gttataatat
     9661 cttttaaagc ttctgtagca agatcaaaag atattaccct attactaaga gaagaataag
     9721 caactactct agttaatgca ccttctagtt cccttatgtt tgacttaata ttttttgcta
     9781 tatatgacat aacctcattt ggcacatcta tacgttctag ttgagccttt tttctaagta
     9841 tagcaattct tgtctcaaag tctggtgctt gaatatcagt tataagtccc atttcaaatc
     9901 tcgatcttag tctatcttct agtgtaggta tatcttttgg cggtctatca ctagatataa
     9961 ttatttgttt gtttgcttca tgaagagtat tgaatgtatg gaaaaactct tcttgagttc
    10021 tttcttttcc tgctataaat tgaatatcgt cgattaataa tacatctacg tttctatatt
    10081 tattcctaaa ttcttcgttt ttgtcgtctt ttatagagtt tataagttca ttagtaaatt
    10141 tttctgaaga aacgtaaact acttttgaat cttttttttg actaacaata tgatgaccta
    10201 tagcatgcat taagtgagtc ttacctaatc ccactcctcc atacaaaaaa agtggattgt
    10261 aagctttagc tggagattct gcaactgcaa cacatgcagc atgtgcaaat ctattgctat
    10321 taccaataac gaacgtatca aatgtgtatt ttggatttag attagggtaa agaactctat
    10381 aatttaaatc tgtactatca gaatttaaat cagcaacttc tttttcgctc aatacaaatt
    10441 taatattata tttttttaat gacagctgac taatagcatc ttcaattaaa tgtaaatatc
    10501 tattctctaa gatatcttta ttaaaatcac taggagccag taatattaaa tcattaagat
    10561 gtatttttaa tggtacgatg tttttaaaaa aggtattaaa acttaccgaa gttaagtcac
    10621 cttttattaa ttgtagggtt ttgtcccata aagaaactat atccataata taaacctccg
    10681 aacagaataa ttaatatttt ccacaaagtt attaacagaa aagcaaaaat atatataaat
    10741 tttacagaat aactaaattt atggataatg gtgttgataa attaataata gtataaatta
    10801 ttaaaaaaat atgaaaagat ataaacaata tgtagataaa gttataaata acataaacat
    10861 acaataaatg gtgattataa ctagagtatt gtcagtaatc ttaaaagtta tacacaaaag
    10921 tatccacaag ctgtgaataa tttctaataa gttgataaca tttatgcaca ttatcaacaa
    10981 gtatatttat aatatcaaaa tatatgtcaa acttcaatga tttgtaacta atttatccac
    11041 aatattaact aattgttgat aaaaatataa acatgttaaa attactttat ttgtgtatat
    11101 aattgaaaaa aataatttag aataaaatta aatagatttt tactagtatt ttcaataaaa
    11161 aaaatacata atatatatat ggtagatttt gtgatataag cgtcccaaaa actcgttgac
    11221 aacagtagta aaagtattta taattatatg tgttatgttt actttgaggg taaagttatt
    11281 atttttataa acggaaaata aaatctgaaa ggcggtgcaa aataatgagt aaaagaactt
    11341 atcaaccaaa aaagagacaa agaagtaaag aacatggttt cagaaaaaga atgaagacgt
    11401 ctaacggaag aaacgtgtta aagagaagaa gagctaaagg aagaaataga ttaactcatt
    11461 aattacaaaa gggccgctgc aaaggtggcc tttttgtgta aataattaca attcatagct
    11521 gtgtttatgg aggttaacta tggactttaa taggactaaa gggttgaaaa aagactctga
    11581 ttttagaaaa gtatataaac acggcaaatc ttttgcaaac aaatatttag taatatatat
    11641 acttaaaaat aaatcagatt atagtagagt aggtatttct gtttcaaaaa aagtaggaaa
    11701 agctattact aggaatagag taagaagatt aataaaagaa gtctacagat taaatattga
    11761 tgaaaaaatt aagcctggat atgatatagt atttatagca agagtatcta gtaaggatgc
    11821 tacttttaaa gacatagaca agtctataaa aaatttagtt aaaagaacgg atatttctat
    11881 ttaattgtat gctataaata tggaggttaa tgactttgaa taaaataatt tatatattaa
    11941 aggaaataag taggtattta tcgaatttat gtatttattt agtgagattt tatcaaaagt
    12001 atatatctcc cttaaaggga cctacctgta gattttatcc aacttgctca caatattcaa
    12061 tagaggcgtt taagaaatat ggatttataa agggtatgta tttaacgata agaagaattt
    12121 taaaatgtca tccttttcat ccaggaggtt acgaccctct aaaataaaag agagtaacta
    12181 ttaggaggta taaaaaaatt gaatataata ggaaatttct taggattgat tcttaaggta
    12241 atatttgaat ttgttaatca ttatggtatt tctataatac tttttacgat acttgtaaag
    12301 attattttat taccattgac aattaagcag actaagtcaa caaaggcaat gcaagatata
    12361 caacctcgta taaaagagat acaagaaaaa tataaaaata agccagaaaa acaaaatgaa
    12421 gaaattgtta agttatatgg agaagctaaa attaatccac tatctggttg tttaccttta
    12481 ttaatacagt ttcctatatt aataggatta tttagcgttt tgagagagcc agttgctcat
    12541 ggcgtttttg caaacaaagc tgcattttta gcagctgaca atgggttttt atggataaaa
    12601 agcttaacat caccagatta tatattggca gtattttcag gagcaagtgc atatgtcatg
    12661 caaaaagtaa tgacaccaaa agatcagtta caaggctcta tgaaagtaat gacatatgta
    12721 atggcaggta tgtctttcta ttggggattt atattcccag caggacttac attgtactgg
    12781 acagtaagta acttattctc aatagctcaa tattacttaa taatgaatcc tttaaaagct
    12841 aaactggctg ctaactctaa ggaggaaaaa gttaatgaga aaaagcctaa aattaataaa
    12901 aagtaagagt aaagaggaag ctataagtaa agctatagca gagttaaatt taaaagcaga
    12961 ggatatagag gtagaaatac ttgaaaatcc aagcaaagga tttttaggtt ttataggtgc
    13021 taaagatggt acttatgaaa tttttgttat cgagagggaa gtagatgtag ctaaaaattt
    13081 tatagaggta atgttgaaaa atgcaaatgt tgatgccaaa gtcaatgtat ctcaaaaaga
    13141 taatttaatc atggttgata ttgaaggcaa agaagctgca agtttaattg gtagaagagg
    13201 ggaaacttta gattctattc aatttttgac tggattagct cttaataaaa taaataaaga
    13261 ttctcataca agagtacttg tagatataga aaattataga tcaaaacgag aagaatcttt
    13321 aataagatat gctaataaag ttgctagaga agtagcgaaa acaagaaaaa ttaaaaaatt
    13381 agattatatg aatccatatg agagaagaat aatacattca gctcttcaaa atgacaaata
    13441 tgtaattaca tatagtgaag gaaccgatcc atatagaaga ttagtaattg agtgtaaaag
    13501 ataaagtcaa cctcctaaac attagggggt tttatttttt aatattatta aatcattaaa
    13561 gattggttaa atataaatct atatgtaaaa aattatgaat taagaggtga taaatttgtt
    13621 tatagatgat acaattgcag caatagctac agcacctgga gaaggtggta taggtatact
    13681 tagaataagt ggagaaagag ctttgaaggt tgcagaagaa atctttaaat ctatgtcggg
    13741 aaagagtatt gaagaataca ataagagaac attaatatat ggaaatatag tggataaaga
    13801 aaatataatt gatgaagttt tattggcata tatgaaagga ccaaactcat atacaggaga
    13861 agatgttata gaaattaatt gtcatggtgg atttatttcg gtaaagaaga tattagaact
    13921 aatactatca aaagatgtta gattagcaga agctggtgag tttactaaaa gagcattctt
    13981 aaatggaaga atagatttat ctcaagcaga ggctgtaatt gatgttataa aagccaaaac
    14041 tgatatagct catgaagttg ctcaaaatca attagagggc tctttatcaa agaagataag
    14101 agaattgaga gataaggtca ctgagatatt agctcatgta gaagttgcta tagattatcc
    14161 tgaagaagat attgagcata taacgtatca aactcttaaa gataaaactg atgaacttaa
    14221 aaaagaaatt aaaaaattat atgatacagc tgaaagtgga aaaatactta gagaaggttt
    14281 aaagacagta attgttggga aaccaaatgt aggtaaatct tcacttctta actccatttt
    14341 aggagagaat agagcaatag ttactgatat accaggaact actagagatg ttatagaaga
    14401 gtttgtaaac ataaaaggta tacctttaaa aattgttgac acagcaggta taagagatac
    14461 tgatgatatc gttgaaaaaa taggtgttga aaggtctaaa gaatcattta ctagcgcaga
    14521 tttgatagta atggttttag atgcatctag aaagttatca gaagaagata tagagatatt
    14581 agaaaaactt aaggataaac aaacaatagt gttgttaaat aagaatgatt tgaaacaaga
    14641 aattgaggaa gaaaaaatac ttaaatatgt agaaaataat agtataataa aaatatcagc
    14701 tttacaacaa gaaggaatag aagaattaca agataaaata gagtctatgg tatataaagg
    14761 tagtatcaaa aataattcta gtttagtagt aacaaattca agacataaag atgcacttag
    14821 taaagcatat aaatctgcaa cagatgcttt aattgctttg gaacaaagca tgccatttga
    14881 ctttgtagaa gtcgatttaa aaaatatatg ggactatctt ggatatatta atggtgatac
    14941 agtaacagaa gatttactag ataatatatt ccataatttc tgtattggta agtaatgtac
    15001 aaaagtaaat ttgtttttat tatagatatt ttttaaaaat ttgtagtgtt tctcatgaaa
    15061 cattaatttt aaacagaaag gaattataaa tgataaagtt tgaagcagga aaatatgatg
    15121 ttatagtagt cggagcagga catgctggat gtgaagctgc tttggctact gcaagaatgg
    15181 gatataaaac tttgataata acaatgtctt tggattctat agcattaatg ccttgtaatc
    15241 catcgattgg ggggactggt aaaggtcaat tagttaaaga aatagatgcc ttaggtggtc
    15301 agatgggact aaatatagac aagacatata tacaaagtag gatgttaaat actgctaaag
    15361 gtccagcagt gcactctttg agagcacaag cagataaatt taaatatcat gaagaaatga
    15421 aaaagacatt agaagatgaa ccaaaccttg atatagctat ggatgaagtt gtagaaatac
    15481 ttcacgaagg aaatgttgta ataggggttg gaactaaact aggatgttca tttaaatcta
    15541 aagctgtaat attggccact ggagtttatc ttaactcaaa gatatatatg ggtgaagttg
    15601 cattttatga aggtcctaat gcactgggtt atgcaaaata tcttacagat agcttagttg
    15661 agcttggtct tagaatgaga agatttaaaa caggaactcc agcaagagtt catagagata
    15721 gtatagactt ctctgtaatg tctttacaag agggagatga aaaagtaact ccattctcat
    15781 ttatgaatga aaatatagaa aaagaacaag aaccttgtta cttaacaaga actacagaag
    15841 aaactcaaaa agttatacta gataatttaa aaagatctgc tatgtatagt ggagtaatag
    15901 aaagtactgg tccaagatat tgtccttcta tagaagataa agtagtgcga tttagtgata
    15961 aaacatcaca tcaactattt atagaaccag aagggttaaa cactaaagaa atgtatatac
    16021 aaggaatttc aactagctta ccttttgaag ttcaattaga tatgtacaaa actataaaag
    16081 gacttgaaaa ttgtaagata atgagacctg catatgctat tgaatatgat tgtgttgacc
    16141 caactcaact aaaaataagt ttggaaataa agggcgttga gaacttattt agtgcaggac
    16201 agttcaatgg gacttctgga tatgaggaag cagcagctca aggacttatg gcgggtataa
    16261 atgcagttag aaaaatagaa ggaaaagagc catttgtatt agaccgttca gaagcttata
    16321 taggtgtctt attagatgac cttgttacta aaggaactaa cgaaccttat agaatgatga
    16381 catcaagagc agaatatcga ctatatttaa gacaagataa tgcagatatg agattaactc
    16441 aaaaaggtta tgatatagga ttagttaaaa aagatagata tgaaagattt ctaaataaaa
    16501 aggctgctgt agagaaagaa tttgaaagat taaaaaatga aagagttaca ccgaaagaag
    16561 taaatagttt gctagaagaa aagggagcaa caccaataaa ggttggaata tctctatatg
    16621 aatttttaaa gagaccagag gttacttacg aattgcttga agaattagga aaaggcgctg
    16681 gagaagatgt ttcaagagaa gttaaagaac agtgtgtaat aataactaaa tatgaaggtt
    16741 atatagagaa acaattgaaa caaatagacc aatttaaaaa acttgagaat aaaaagctgg
    16801 atgagaaaat aaattattca tctatagaag gtcttagatt agaggctaga cagaaattgg
    16861 atgatataaa acctatttca ataggtcaag catctcgtat atctggtgtt tccccagctg
    16921 atatatcagt tttacttata tacctagaac aagtcagaag aactagaggt ggcaaaggtg
    16981 aataatatgg aacttctgaa aaatgggatt gaaggtttta atatagatac gaatgatagt
    17041 atgttagaaa aatttaaaat ctatagagag atattaattg actggaataa aaagatgaat
    17101 cttactggaa tagaggatga aaaagaagta tatataaaac attttttaga ctctatttca
    17161 gctgtaaaga atggatatat aaaaaatggt atgtctataa tagatgttgg tacaggtgct
    17221 gggtttcctg gaataccatt gaaaatatgt ttagatagat tagaacttac tttgttagat
    17281 tcgttaaata aaagaataaa ctttttagag gaagttagta gagtattaga gttagataat
    17341 attaaattta tacatggaag agcagaagat tttggtaaaa atgaaaacta tagagaaaaa
    17401 tatgatgttg ctacagcaag agctgttgct ggattaccaa ttcttatgga attttgtgta
    17461 ccctttgtaa aagttggagg atattttatt tgcttaaaag gtccaaatgc taatttagag
    17521 ttagaagaat ctcaaaaggc tattgatgtt ttaggcttag agtatattga aaaaatagat
    17581 gtagaactac cagaaataga tttaaatcat aatatattag tttttaaaaa agtaatggaa
    17641 acaccagtta agtaccctag aaaggctggt aaaccagcta aaaatcctat taagtaatga
    17701 aaaaattatt agcggtaagc ttaattattt ctcaggaaat aattaagctt atcgtaaaaa
    17761 aacaaaaata aaggaattat taaagtagat gcagaaataa atttgtatgt acatatagag
    17821 aacaaaaata atttatatct aaaataagag aggtatgtta tggaagataa aagagttatg
    17881 gagataccta tagaagatgt agttccaaat ccatatcaac caagaaaaat attttcacaa
    17941 gtttcattgg aggaattgag taattctata aaggtatatg gtataattca acctataaca
    18001 gttagagcaa aagatggcaa atatgaacta attgcaggag aaagaagatt aagagcagct
    18061 aaattagcag aacttaaaac cataccagca attataaata atatgaatga tgaatcttct
    18121 gctgttctag cgttattaga aaatttgcaa cgagaagact taaatttcat tgaagaagca
    18181 attggttatg aaaacttaat aaaagaacat gcttttactc aacagcaatt agcagaaaaa
    18241 ttgggcaaaa atcaatctac tgtagcaaat aagcttagaa tattaaagct tccaaacgat
    18301 ataaaaatga agctaataga gaataattta acagaaagac atgctagagc atttcttaaa
    18361 ctaccaagtg aagatttaat gcaaagtgtg ttagacaaag tcataaaaaa tgaattgact
    18421 gttaaaaaaa cagaaaaatt gatacaagat gtattagaag aaactaaaat tcaagaagag
    18481 cctgataaaa aacagaatat aaagggtgct atgagtataa gaatttacat aaatactata
    18541 aagcaggcct ttgatgctat atcaaataca ggaattgatg ctaagtataa tgaaatagat
    18601 aagggagatt atatggaggt tgttgtaaaa atccctaaaa aatagagatt actgcttacc
    18661 agtaatcttt ttttgtaata taagccttgt aatataaatt gatttataga cattatttat
    18721 acttaaaaaa cacctattca acatttaaaa ttaatttaat tcttaaaggg gggaacatca
    18781 tgggcaaagt tatagctgta ttcaatcaaa agggtggagt tgggaaaact acaacaaatg
    18841 taaatttaag tgctagttta ggaactttag gaaagaaaat attagtatta gatttagacc
    18901 cacaaggaaa tacaactagt ggatatggta ttaataaaaa tgaagtagaa aatacaatct
    18961 atgaaattat gttagatggg ttacatatta aagaagcaat aataagtact gaatttgaga
    19021 atgtagatgt agttccatca gctactgaac tatcaggtgc agagatagag cttacatcta
    19081 aaactaatag agagtatatt ttaaaaaatt ccataaaagc agtaatagat gaatatgatt
    19141 atatattttt agattgtcca ccctcactag ggatgcttac aataaattgt ttaactgctg
    19201 tagatagtgt tttaattcca atacaatgtg aatattatgc tttagaaggt gttagtcaat
    19261 taatggagac tataaaatta gtaaaatcaa gactaaatgc tgatatagaa attcaaggtg
    19321 ttgttttaag tatgtttgat ggaagagcta acttatcaat acaagtagtc gaagaagtca
    19381 agaaatactt taaagggagt gtatatacaa ctctaattcc aagaaatgta agacttgcag
    19441 aagctccaag tcatggaaaa cctgtaattt actatgataa aagatgtaga gggtctgtag
    19501 cttatttgga actagcagaa gaatttatag atttagaaga ggaggaatgg tagtatggag
    19561 aataagtcta aaagaagtaa tagattgggt agaggtctta gtgcattgat accagaaatt
    19621 aaaggagaaa ctccagaaaa agaaatagtc aacatagata tagacaaaat atatccaaac
    19681 gaagttcaac ctagaaagca atttgatgaa gaaaagataa aagtattggc tgactctata
    19741 aaaaattatg gtgtattaca gccaatagta gttaaaatgg atgaaaataa taaatacatg
    19801 ataatagctg gagaaagaag atttagagca tcaaaacttg caaataaaaa tcaaatacct
    19861 gctataatta aagatataga tatgaaagat ataatggaga tagctttaat tgaaaactta
    19921 cagagggaag atttaaattc aatagaagag gctctagcat ataagagttt aatagaacat
    19981 tacaatgtaa ctcaggaaga gatatcagaa gctgttggta agagtagacc tcatataact
    20041 aacacactaa gacttttaaa tcttggacaa gatgttatag aaatgataga cagtggtaga
    20101 attacagcag gtcatggtaa ggctttattg agaattgcag ataaagattt gcaacttcaa
    20161 atagcaaaaa aaatagaaga agaagaactt tctgtaagag aagttgaaaa tatagctaaa
    20221 aaaatatctg aaaataaaca agaagaaccg aaaaaatcta aacctaagga tgtatttatc
    20281 ttagatgttg aagataaact tagaaatata tttggaacaa aggtgaatat ctctaaagga
    20341 aagaaaaaag gtaaaataga gatagaatac tataatgatg atgatttaaa tagtattgta
    20401 tcaatgcttc ttgaatagaa caaaaatata aaacagagag gaagattgtt atgatttatt
    20461 tagataatgc tgcaactaca tatccaaaac cagaaagagt gtataatgct gttttagatt
    20521 gtatgaaaaa ctattgtgca aacccaggta gagcaggtca taaacttgct atgagagctg
    20581 ctagggagat atatgacaca agagagaata tagctaagtt gttcaatgta agtaatccta
    20641 tgaatatagt atttacatct aatgcaacag attctttaaa tttagcaata aaaggcgtat
    20701 tacaagaagg agaccatgta ataacaacat ctatggaaca taattcagta ataagaccta
    20761 taaaatcatt ggaaaaaaaa ggtattgaaa atactgtagt aaaatgcgac tatgaggggt
    20821 ttctaaatta tgaggattta gaaaaagcga taaagagtaa tactaaattg attgtaacaa
    20881 cacatgcatc taacgtatgt ggaactttaa tagatataaa aaaagttgga gaaatagcta
    20941 aaaaacataa tatactattt ttagtggatg catcacaaac agcaggtgtt tataatatag
    21001 atgtaaatga atgtaatata gatatgttgg caatgccagg tcataaatgc ttatttgggc
    21061 ctcaaggaac aggtatatta tatgtaagag aagggttaaa cttaaatata ttaaaagaag
    21121 gtggaacagg tagtaaatca gaagaaatag ttcaaccaga gttattccca gataaatatg
    21181 aatcaggaac tcataatact ccaggcatag caggtcttaa ccaaggtata ttatttatat
    21241 ttgaaagagg aatcaacaat ataagacaac atgaagaaga attatgtcaa tatatgatag
    21301 acaaattaga ggaagtccca gatataaaga tatatggacc aaaagatagt aaaaaaagag
    21361 cagcagtaat agctctaaat ataggaaata tggattctgg agaggttact ttcttattag
    21421 acagtgatta taatatagca accagatcag ggatacattg ctctccacta gcacatacca
    21481 ctttagggac tttaaaacaa ggagcagtaa ggtttagtat aggatatttc aacacaaaag
    21541 atgagataga taaagcagtg gaagctttga aaaaaatttc taaaaataaa tagcatgaaa
    21601 tttaagaata tataaaaata aagaaaggag atgtttcaaa ataaaaattt gaatcatctc
    21661 cttttttgta caaaaaagaa aaggggttcc taagaatccc taatctattt gataattgtt
    21721 tcaagaaatt attttaagta atctccttct tcaagaacta tatgaccact ttcataatta
    21781 gaaccatcaa cagtgatttt tatttgttca acattcatat tttttatgta agtacgagca
    21841 actgaatcaa gcattaaagc ttctgcatca ctacctaaat ttgactttgt aaaatcggag
    21901 ctaacatcta atataccagt ttttacacca tcaatgtctt ttgtagtaaa tgaatttaat
    21961 tttgcagatt ctggaacaac tttaagtttt tgaagttctt caaataaagt gttttcatca
    22021 attttactta aatcaacctt gttttcaatt aaatctgtct tatctacatc aaaagagtaa
    22081 atagtagctg attgagtttt agtttcttct tttggagtag gtttagtttt gttattagga
    22141 acctcttttt cagtactatt ttcttctttt ggttgttcaa catttgtatt actcttagtt
    22201 tcttttttag atgtatcatt tccattttga gcattagtac aaccaactgc gaatatagat
    22261 atagataata tacttaacac taacattatt ttcttatttt tcataaaaat ttctcccttc
    22321 ttaaaaacta ttcttatata taattatagt cgttatgagc aaatatatga atacaaaata
    22381 gtaacaaatt taaaaaatga tttatttaaa ttaattaaat tcaaaataaa ctaaatttta
    22441 gtcattttta tgggaaatca tttaatgtac actgaaaata ttgtaaaata aaagtagtgt
    22501 ttttaaaata aatataagtt ataattatat tataaaaaaa tgtcttcaaa aggtgggaat
    22561 agtatggaaa ttttgagctt aggagaaaag attaaaaaat taagaaaaga aaaaaacatg
    22621 acattaaaag aattagctgg agataggata acagcagcac aaataagcca tatagagagg
    22681 gataaatctc atactagtta tgaactgttg gaatatctag gcgataagct agatgtaagt
    22741 gtagaatatt tattagagac caaggaaatg caatcaaaaa agataactga taatttaata
    22801 ctacagagcg aaatttatat aaaagaaaat gagttaaaca aggctgagga ccaaataaaa
    22861 gaagttttag atatatgtaa gagatacgat ttaatggaga actatggcag atgcaatttt
    22921 ttaatggcta atattaatct gaaaaaatct aattataatg atgctataga aaactttgaa
    22981 aaggcattgt acttctttat aaaaaatagt gatagtgaaa atatacttaa gtgttattta
    23041 aacataggga aaatatatat gcaggaagac ttttataaag gtgctataag ccactttaat
    23101 tttgcagagg aaattttgtc agagaataaa tttgaggatg ttaacataca caaggaacta
    23161 tatagtaaga tggcatattg ttacataaaa ctagataatc caaatatgtc attgaaatac
    23221 atagaaaaaa taaatgaaat agataataga aatgatagaa aagaagatgt cgatattttg
    23281 gtattaaaag caaataatat gcttgaaatt gggaaatatg aggaatcaag agattacttt
    23341 aaaaaagcgc taaaaatact tgaaagtgaa gataataaaa ctggattagc aaatgtgtat
    23401 ttaacaattt gtgacgttta cagaaaaata ggtgaaacag atagagtact tgaatattca
    23461 caaaaggtat atgacataaa gaaaaatgat gaagatgaat atatgatgtc aggattattt
    23521 aagataatag aagcatatat agataaagaa gattatgatt tagctagaaa atattgtaag
    23581 atagccttag cttcatcaat aaaaaataaa aataaattca atgaatataa agcattgaga
    23641 ttctatgcta atatgtataa aaatcaaaat gaaataactc ttgcaattga atatttgatt
    23701 aaatgcataa aaatagtatc agatttagga aacaatagaa tattggccaa tttatatata
    23761 gacttaggtc aattatattc aaatatatca aaagaaaaag agttagaata ttatcaaaaa
    23821 ggtgtattta tgtacaaaaa cttagaaata atgtagagta aataaaccta taagggtata
    23881 gaaacaatct ataacaagca agataatatc tatgataaaa tataaattgt atgaatattt
    23941 ttaaggagat gaatcagaca atgagtataa aaatagatgc aagagggctt gcttgtccta
    24001 aaccagttat aaatacaaaa agagaattgg ataaactaga agaaggtgtt gtagttacaa
    24061 tagttgacaa tgaaacagct aaggagaatg tattaaaact tgccaaatct ttaagttgtg
    24121 aagcaaatat tgttgatgaa aaagagaatt ttatatctat agaaattaga aaaggtaaga
    24181 atatactaga taaacaaaat attgtagata ataaagaaag tgaattagac aatacatgta
    24241 tatttatatc ttcagataag atgggacttg ggaatgacga attaggaaaa gtgttaataa
    24301 aaggatttat atatacatta acagaatcta aaccttatcc taagtatgta cttttagtta
    24361 atggaggagt aaaattaagt gctgaaaatg aagaaactat agaaaatcta aaaatacttg
    24421 agaatatggg tgtagaagtt ttatcttgtg gtacttgctt agattattat aatttaaaag
    24481 ataaattaca agtaggtaca gttacaaata tgtatactat agttgagaca ttaaagaatg
    24541 catcaaatac tatttctata taatgtattt ttgtttagta atataaaact aataataaag
    24601 attaactcaa atagatagtc agaaaggatt aagtaatgaa tcaaatgtat atagtgtcat
    24661 ttaattcgac acatcatgcg ataagatcag aaaagttatt tggtgaaaat agtctaaaag
    24721 taatggctct tccaacacca agagaaataa cagcaagttg tggaatatca attaaatttt
    24781 cttttgaaga tatggaaaag ataaaaacta ttttaattga aaataatgta gatataaaag
    24841 gaatttattg tataagtaaa ttagaaaatg gttcaaaaga agtagagaag ttagattagg
    24901 aggctaatta tttatgccaa tggatttaaa aataggtgat attgttgaat taaaaaagca
    24961 acatgcatgt gggtgtaaag agtttgaaat cataagaaca ggaatggata taaagataaa
    25021 atgtactaaa tgttcaaggc taataatgct tgatagagag accttagaaa agagagtaaa
    25081 aaagctaata aaaaagtgat aaaaatttaa cacttaaaaa atgtcaaata ccgtatatta
    25141 atatccaaaa ttttggtata ataagaattg aagaaaacat tttcatgggg gtgctttaat
    25201 tatggcagtt aaatatgcaa aaagaatgca aggtttacaa ggatcagaaa tacgtgagct
    25261 tttaaaactt actcaacaac cacaaataat atcttttgct ggtggaatgc cggctccaga
    25321 gttatttcca gttgaggaaa tgaaaaaagt atcagttgca gtgcttgaag aaaatggaag
    25381 atcagctatg caatatacta caacagaagg atatgaacca ttaagagaaa aaatagcagc
    25441 tagaatgaat gataaaaaca aaactaatgt aaacaaagat gatatattag ttacaagtgg
    25501 ttctcaacaa ggtctagatt ttgcaggaaa agtatttatt gatgagggtg atgtaatttt
    25561 atgtgaaagc ccatcttaca taggagctat aaacgcattt aaatcttacc aacctaaatt
    25621 catagatgtt ccaacagatg ctgatggaat gataatggaa gaattagaaa aaatactaga
    25681 aacaactgat agaataaaaa tgatttacgt aatacctgac ttccaaaatc caacaggaag
    25741 aacttggcca cttgaaagac gtaaaaaatt catggaaata gtaaacaaat ttgaaatacc
    25801 agtaatagaa gataatccat acggagattt aagatttgaa ggagagactt taccatcttt
    25861 aaaatcaatg gatacaaaag gattagtaat attcttagga actttctcta aaatattctg
    25921 tccaggatac agattaggat ggacttgtgc ttctccagaa attttatcta aatttaactt
    25981 tgcaaaacaa ggtgcagact tacaagcatc aactatatct caaatggaag taagcaaatt
    26041 tatggatatg tatgacttag atgcacatgt tgacaaaata aaagcagttt atgttaaacg
    26101 tagagatgta atgcttaaga ctatggaaga agaattccca gaaggattag tatttactca
    26161 tccagaaggt ggattgttta catgggtaga gcttccaagt aatttaaatg ctaaagaatt
    26221 aatgccaaaa tgcttagata agaatgttgc ttatgttgct ggaggaggat tcttccctaa
    26281 tggtggtaga gaaaatactt tcagacttaa ctattcaaat atgccagaag aaaaaataat
    26341 agaaggtata aaaaatatag cagctgtatt aaaagaagct atgggtgtag aagcttaatt
    26401 aaaaataata acttatatat aatacaaggg atggttattg ccatcccttg tttgtttgaa
    26461 aaatatataa tctaccttac actaaacttt gtccaaaatt taaattgaca gaaataagaa
    26521 taaatgataa aatgtgtagg tatgagattt tagtctcatt tctctgctct aagagtgtag
    26581 agccgttaag tccaaaggga ggtgaattat agtgagaaat tatgaattag tttatgtggt
    26641 aaagccaaac tctgatgaag aagtaagaga ggctatacta aacaaagtta aggaagttgt
    26701 tgcaactgac ggagaaatag ttaaagttga tacttgggga actaaaaaat tagcttatcc
    26761 aatagctaag tttacagaag gtttctatgt attagttaac ttcaaatcag cagtagacgt
    26821 tcctaaagag atagacagaa acttaaagat aaatgaaaac gtaataagac acatgatagt
    26881 tgttgcttaa taactggttt tattataaaa aataggtggt gtttttatga atcaagttgt
    26941 attagtggga agattgacta gagaccctga attaagatac atcccaggta caggaacacc
    27001 agtggcatcc tttacaatag ccattgatag agactatgta aaaaaagatg gatctaaaga
    27061 aactgatttt atacctgttg aagtaatggg taaatctgcc gaattttgtg ctaactacat
    27121 aactaaaggt agattagtag cacttcaagg atctataaga gtagacaatt atcagacaca
    27181 atcaggtgaa aagagaactt ttacaaaagt aagtactaga tcagtacaag ctttggatag
    27241 taagaataaa tcggaaaatt catatagaga gagtgctcca gcttttgagc caagctttga
    27301 acctcaaggt ttagacccac aaggtttcca agctatagat gatgacgata taccatttta
    27361 aaaaggaggg aaaaaagtca tgatgaataa aaaaagacgt aagaaaaaaa gagtttgtca
    27421 attttgtgct gacaaaaacg ctaaaataga ttacaaaagt actcaaagat tacaaaagta
    27481 tataactgaa agaggaaaga tattaccaag aagaatatct ggaacttgtg ctaagcatca
    27541 aagagaatta acagttgcta taaaaagagc gagaaatata gcacttttac catatacatt
    27601 agactaaaaa aatgagcctt gtgctcattt tttattatat ggatttatat atctaaatga
    27661 ggcgagctat aaagctcgtt tttttgttta tatacttaaa tatattatta tttatgatat
    27721 aatattatat attaaggagg gttgtaaacg tgaaaataga taaaggttct gagataacaa
    27781 gaaacataaa gataatagaa tggatgaaaa cagaaatatt aatgagtgta agtgatttgt
    27841 ttaatttatt gtttaaaggg gtaaaaccat tagatgaagc aattcaagat actttagcaa
    27901 atataataat gataacatat ctcttagcca agagattagg aatcagtttt agagatgtgg
    27961 attataaagt caaggaaaaa ataaaaattg gtatagatga ggaccatagt gttgaaagat
    28021 ggtatggaga tttatctaat ttaaaaaaac atatggataa tagggagtga tattaaattg
    28081 aataataaaa ttagactatc taaggcaatg tctataataa ttttagctat aataatcgct
    28141 ttagtaatag cctatattcc aatgctagga atatttagta tgttagcagc tgttccatat
    28201 gtaattatag gtgctattac tgatagaaag tattctctta tatctatttt ggttacattt
    28261 tgtgtactga ttctcttttc agacatttct tatgcattaa atatatgtat aatgtattct
    28321 ttaccaggtg tagtaatagg aaagatgcta aagagaagtt ttgaacaaga ggacagtaat
    28381 aaattcgaac cgatatatgg tggaaccata atatttgtac tttcaatgct tgtatatttt
    28441 tttgtattaa agacattttt aaatgtgaat ctattagatg aagtttctaa gatgatatca
    28501 gaaattgtaa atattcaaaa aaatactttt tctgcaaccg agcttaaagt gtttgataaa
    28561 atgaagccag atgagattgt aagttatttt acaaatatgc taccaatgat gttattttta
    28621 caagggttgc tatcagcgtt tgtcacatat tatttgagtg tattttttat aaaaaggatt
    28681 acaaagatga atataagatt tcctaaattt gctgattttt atttaccggg aaatgctata
    28741 ctcacaactt tcttacttta tttattggtc ttatttattc aaattattgg ttcaagacta
    28801 tacacagaat tgataatgac taatttacag ctagttttta atttaatgtt tgtaattcaa
    28861 ggtatagcag tttgcattta ttttttaaag aaatggataa gacagggacc aaataaaatt
    28921 atgttttttt cagggataat tttatgttta tttggattta tgggaatatc ttttgttggt
    28981 atggtagata gtataattga ctttagaaag gtaagaagct ataaatccac ttaggaggat
    29041 agaaatgagt aataaacaaa cctttaaatt aaatatgcca gaaataaatt tatatataat
    29101 tgttattggt atttctagta taattttgct ttattacaac ttgtatgtag ggtgtttatt
    29161 tttctgtatt tttgtatata tggttttcca taattggaga acaactaata tcagacatca
    29221 tgaatggaca gagtatattc aaaatttatc tttagatata gatgaaacaa ctaaaaaagc
    29281 cataataaat cttccaatac cattatgtat tttagaattt gatggaaata tatcctggta
    29341 taatggaaag ttttatgata tgattggtca aaaagattta ctagataaaa atatagaaga
    29401 tatagttaaa aatcttaatt taagaaaagt attaaatgaa aataaagaga tgtacacaga
    29461 aataaactat aaagaaaaag aatatacaat aatttataat gttataaaaa atgatcaaga
    29521 aaaaaatcct aaatatttga tgatattata ctggatagat aagactgaat atttaaaagt
    29581 aaaacaaaat tatgatgatg aaaaaaatgc aatgatgcta atacaagtag atggatatga
    29641 tgaagtatta aaaagtgcag cagaagataa gagagcttta attaacgttg aagtagagaa
    29701 aatactttct gctttagaat taaattcaaa tggtgcattg agaagaacct caaaagacaa
    29761 attcttcttg gtaatgcata aaaaagagtt aaaaaaattg gaagctgaga agttttctat
    29821 tttagataca ataagacata ttgattatgg aaataatctt cctgttacta taagtatagg
    29881 aattggtata gatggagata cattaaatga gaacctaaaa cttgctacag gtgctctcga
    29941 tttagcactt ggtagaggtg gtgaccaggc tgttgtaaaa acaaaagata aatttgtgtt
    30001 ttatggagga aaatctaaag ctgtagaaaa gaagaccaag gtaaaatcta gattaatagg
    30061 tcatgcttta agagaggtta tacaacaaag tgaccaagtt tatataatgg gacataaata
    30121 tcctgatatg gatgctatgg gagcagcagt tggtgtttat gatatatgta aatcatgtaa
    30181 caaaactgca aacatagttt tacaatctgt aaatgaatct atagaaatat ttataaataa
    30241 aataaatgaa aataattact ataaaaaatt gtttattggg aaagaggagg ctattgacaa
    30301 ctgtactaaa aatacattag ttgtagtagt tgatactcat agacctaatt atactgaatg
    30361 tgaagagcta ttaaagctat cagaaaaggt agtagttata gaccatcata gaagaggtgt
    30421 ggagtttata aatgatgcag tgcttttatt ccatgagata tatgtatcct caacgtgtga
    30481 aatggttaca gagttagttc agtatatgga tgaagatgta acaataaata aacttacagc
    30541 tgaaggactt ttagcaggta taagtctgga tactaaaaac tttgcattca aaacaggtgt
    30601 taggactttt gaagctgctt cttaccttag aaaagttgga gcagatacta tagaggtcaa
    30661 gaagttcttc aactctgatg taaaagactt cataataaaa gctgaaataa ttcaaagtac
    30721 taaaataatt aataatagaa tatgtttagc atattcaagt actgagatag atagtataaa
    30781 tgttataata gctcaaactg ctgatgagtt actaaacatc aaagaagtag aagcttcctt
    30841 tgtattaggt gaaaaagatg atactatatt tataagtgcg aggtctttag gacaaataaa
    30901 tgtacatgtg cttatggaaa aattaggagg cgggggacat atagacatag ctggagcaca
    30961 gttaaaaaat gtttctctaa aagaagctta taagatggta aataaaataa tagaggagta
    31021 tttggaggag gaagaataga tgaaagttat attattaaaa gatgtaaagg gaactggtaa
    31081 gaagggagaa atgaaagaag taagtgatgg atatgcaaga aatttcttat ttcctaaaaa
    31141 gatggctgtg caagcagata gtgtagcaat aaaagaatta aatgaaaaga ataaatctaa
    31201 agaaataaaa gcacaaaaag agtatgaaga agcagtttta ctaggtaagc aaatggaaga
    31261 aattaatata gaaatatatt caaaagcagg agaaggtgga agattatttg gttcaataac
    31321 ttctaaagaa attgctgaac aattaaagaa acaaaaagat atagatgtag ataaaagaaa
    31381 aatattatta gatgaaccaa taagagcttt aggttcaact tttgtagaaa taaaaataca
    31441 tcaaaaggta actactaaaa taagagtgga tgtaaaagaa aaacagtaat aattagaggt
    31501 gattaagatg gaagatatga cgagaattcc tcctcatagt gtagaatcag aacaatcaat
    31561 attgggttct atacttttag ataaagatgc tataataacg gttacagaaa ctataaagcc
    31621 tgatgatttc tataaagaag ctcacaaaat aatatatgaa tgtatgataa ctttaagtaa
    31681 taaaggtgag cctatagact tgataacatt aacagaagaa ctaagaaaac aagggcatct
    31741 taatgatatt ggaggaatca gttatattac aagtctttct actatagtac cgacgacttc
    31801 aaatgtaaaa tactatgctg acatagtaaa agaaaagtct gtacttagaa agcttataaa
    31861 agcttctaat gaaataataa atctaggata tagtggagca actaagattg aagatgtttt
    31921 agaacaagct gaaaagagta tatttgatat atcacaggag aaaactagtg acgattttaa
    31981 atctataaac ttagttttga tggatgccta tgatatgata gaaaaattat atactaacaa
    32041 gagcgatgtc actggtataa ctactggttt taaagattta aacaaaaaaa taaatggtct
    32101 acaaaggact gatttaatat taattgcagc tagaccagca atgggtaaaa ctgcattttc
    32161 tttaaacttg gttcaaaatg cagccttaaa aggtgatgct tctgtagctg tatttagttt
    32221 agagatgtct aaggaacaat tggtacaacg tatgttatct tcacagtcta gtgtagaatt
    32281 aaaaaaaata aagactggaa cacttaatga taatgattgg cctagaatta tagatgctat
    32341 ggcagtgtta tcagatgcca aaatacacat agatgatact cctggtataa aaatatcaga
    32401 actaaggtca aagtgtagaa aattaaaaat agaaaaagga ctagacttag tgcttattga
    32461 ttatcttcaa cttatggaag gtgaaggtaa taatgaaagt agacaacaag agatatctaa
    32521 gatatcaaga tcactaaaaa tattagccaa agaattaaac tgtcctgtag tagcactatc
    32581 ccagttatca cgtgcaccag aacaaagagc agaccataga cctatgttat ctgatttaag
    32641 agagtctgga gcaatagaac aggatgcaga tatagttatg ttcttatata gagacgaata
    32701 ttatcatgct gattctgaga gtaagaatat aggagaagta ataatagcaa aaaatagaca
    32761 tggtgaaact ggttctgtgg aacttgtttg gcttggtgaa gttcaaaggt ttggggataa
    32821 gttgagagac ctataatata acgaatatcc acagaacgtc ctttctttct acaggaaaaa
    32881 tgtggataca tgtcactaaa tatgtctaaa cattgaaatt tagctaaaaa attatccaca
    32941 gtatccacag atagcctgtt gataagtgtg aataagtaga taaagcaagc ttttggcttg
    33001 ctttatctgt gtataaatat taattattag gaggaataat atggaaaaaa taagcttaaa
    33061 atatatatat cccaatataa ttaaagtact tgatgaaata aacttattta gagtaattga
    33121 taataattta agagaatcta ttgttgttta tgctaataat gtggataatc agtatcatat
    33181 aaacatgact aatacaaact ttggaaacat aattaacata tgtaaattag aaaaactttt
    33241 agatgtggat aagtttatgg aaaaagttat aaaatcagaa aaagaaataa tagagaaaga
    33301 agagttttca aaaatagaag aatatatgtt aaatattgga gaatactaaa aaagttgaaa
    33361 aaaatacgaa tgtttgatag aataagtaag tagagtgttc gagagtatat aaaatataga
    33421 ggtgaattac atgaaaacag ttgcaattgt tggttctcaa tggggagatg aaggtaaagg
    33481 taaagttata gattatcttg ctactcaagc agatgtagta gtaagaggac aaggtggaaa
    33541 taatgcagga catacattag ttgtggaagg taaaaaatac gcattacatc taattccatc
    33601 tggagtttta aatccaaata cagtgaatat aatcggaaat ggaatagtgt ttgaccctaa
    33661 aggattttta gaagaattgg aaatgtttaa aatggataat ataagtacag aaaatataaa
    33721 aataagtaat agagcacatg tcatattccc atatcataaa gaactagatg tattgtcaga
    33781 agaagccaga ggcgatttaa agataggtac aacaaaaaaa ggtattggac cttgttatat
    33841 ggataaaaca gagagatctg gtattagaat atgtgactta atggataaag ataaattcgc
    33901 tataaaatta aaagctcaaa tagatgctaa aaatgaaata gttaaaaata tatatggaaa
    33961 agaagaatta tttgattttg aaactatata taatgaatat ttaggatacg cggaacaaat
    34021 aagaaaatat gtggcagata catcagttat tgtatatgat gcaataagag caggtaaaaa
    34081 agtattgttt gaaggagcgc aagggacttt attagattta gatttaggaa cttatccatt
    34141 tgttacatct tctcatccaa catctggtgg atttgcaatt ggtgcaggta taggaccaaa
    34201 tatgataaaa gatgttgttg gtatagtaaa agcatatact acaagagttg gagaaggacc
    34261 atttgttaca gagcaaataa atgaaactgg agataaaata agagaacaag gtcatgaatt
    34321 tggggtaact actggtagac caagaagatg tggatggttt gatgcagtta tagttaagta
    34381 tgcagctaga gttaatggat tgactagtat ttcatttatg ctacttgatg tattaactgg
    34441 atttgataag ataaaagtct gtacatctta caaaatggga gataagataa taacagattt
    34501 cccagcttca ttagatgatt tagcaaaatg tgaacctgta tatgaagaat tagatggatg
    34561 gaatgaagat ataactcaaa tagataactt tgatgacctt ccagaaaatg caaagaaata
    34621 tgtagccaaa atagaagaac tagttggagt aagtgtagat atggtttctg ttggtccaaa
    34681 tagagctcaa accataataa gaagaaatat atttgcttaa tattttatca attagataat
    34741 ttaaattgta aatagttgaa gaagagcatc ttaagataat gtttataatt attagagatg
    34801 ctcttttatg ttcgacttaa atttattttt tattaagtta aaattactga cacacattct
    34861 aactgaaatt aaagttgaat ttgtgtagag atgtataggt ttttaaaaag aattttgttg
    34921 aaaataaata gctaaagatg attttaatta tatgctaaaa aaaataaaaa aaactattga
    34981 ctaaaaattg tatagaagct ataatataac ttgtgagtga aagaaacgac gaaaaaagtc
    35041 gaaaaaatgt ggtctattag ctcagtcggt agagcacctg acttttaatc agggtgtccc
    35101 gcgttcgagt cgcggataga tcaccaaaat atggctcgtt ggtcaagagg ttaagacacc
    35161 gccctttcac ggcggtaaca ggggttcaat tcccctacga gtcaccagta tgggactata
    35221 gctcagctgg gagagcacct gccttacaag cagggggtca caggttcgag ccctgttagt
    35281 cccaccatat ttttaattag agataaaaac tagattatat gctggtgtgg ctcaacggta
    35341 gagcagctga cttgtaatca gcaggttgta ggttcgattc ctatcaccag ctccacaaag
    35401 tacataaatt taaattaata taattttcta tgttacagga tacttttaag tatctttttt
    35461 taatttcatt ttttattgta ttaaaaatga aatttacata taacaaaata ttgaattact
    35521 tgtaatgtga tagttttgaa tctaaaattt agaaacattc tagaatagta ttatgtaatt
    35581 agcaaattta atttatagta ccaataaata tagattgcta agaatataaa tgatttgtta
    35641 ggttaagata cattagatat gtaaatagaa aaataaaaag tatggtttaa tttatgtggt
    35701 ataagtttta aacagagaag caagtttgct cttacctctc tatttaaaaa taaaaaaatg
    35761 ttttttaagg gctattttat ttgaatcttt cccatctcaa atcatctcca gtaaattcta
    35821 aaatcctaaa cttatccaag attctagaga gagttctttg agtataagta tttcctatct
    35881 gagatggtgt taagttggtt gaaataatga tttttttacc agcaacaagt cttgtattta
    35941 caatattaaa gatttctcca ctagtaaaag aattattaag ttcagtacca aggtcatcta
    36001 ttattaataa atcgcaatca aacaagtttt tataattatc ttcacttatc tgattatttg
    36061 agtctctcct aaatttataa tcttcaagaa tatctaagat tctaaaagaa gtttgataga
    36121 ttacaacatt gcctttatct aacaattcct ttgcaataca attgcacata tatgtttttc
    36181 ctagtccagt agaaccataa aacagtaaat tttcactatt atctttctta aaatcatgaa
    36241 caaaattttc gcagatactt aaattgttta gcatattttc tctaggagat atttccccat
    36301 cagaactttt cttaggagaa aatatattca agttaaaatt cgaaaaattt tcctgactta
    36361 aaatcctatc taaattagac attttataag cttcatttac aatttcttgt ttaaggcaag
    36421 aacatttttc accattaggt aaaaatcctt tatctttaca agaaacacat tgaaacttta
    36481 tttccaaata atcaagaggt atgttatttt cagttaaaag tttttctttt tttaatttta
    36541 aagactctat attctcttta gtcttcttta caatttcatc tttagattta ggatttaaaa
    36601 gtactatttt agctaatctg agacctattt tagaaatttc atcatctaaa gattttattt
    36661 cagggattct attatatacc tcattttttc tatgttctaa taagagttca ttatcatctc
    36721 ttctcttagc atatttagca agtatttttc ttattttatc ctcattcatt taatcaccta
    36781 cttaaacttt tctttttgac tttttttaat aatctcatcg agctcatcag atgtatattg
    36841 agtgaatgtt tcgttgaaat tatgaaactt agttttcttg taagtattgt tgttttgagt
    36901 agtattaatc tgcttcttat taatattctc cttaactata cgttcttcct ctttttgctt
    36961 taaatcattt aaatttttta tatttttttc attccaattt ttaattatac catttatata
    37021 agatatactt gggtttgaaa tgttcttaga cttggaacat gcttttatta tcaaatctat
    37081 atccatatta aatttatcaa accaagtgtc cataagttct ttttctgact ttgatggttg
    37141 tcttgaaaat cccaattcat tgaaaatcgt tttatataaa atatatcttt cacttcttac
    37201 caaaaagctt tcttcaacat cttttggtgt atataaattt gaatcatacc aatttctaat
    37261 aataccttca atatatttaa ctggctttga tgtaccagtt ttatctttga cgtattcata
    37321 tgcgcaaagt atcatatcag ggctcatatt gtatttattc attatatcca taatactcat
    37381 cttttcactt gggtcgagat atcgcccaac tattttattt atagagttaa acatttttct
    37441 aatgcttgga ttttcagaag tagatacaac cttatctgta ttagatttta cagatgaatt
    37501 attgcttaat atattctcaa tatagaaatt ttttaaatct aaaaattcta ttgaataatt
    37561 aaaattatca tattctccat tgtcatgtat ttttataatc ttcttttcct cccagaattt
    37621 ccaagcagat aacacatcag acaatggtat atttaggttt ttggctattg aactattatc
    37681 gaacttagga ttagaagtaa tgtcgcatgc ctgtctatat cctaaaagat aaacttttac
    37741 gtataagccg tccgccattg gcataaatat atctataaag atattgggaa tagtgatttc
    37801 acctaaatct atttcattgc tttctttaaa aaacatttta tcacctcaga ttaaattata
    37861 tcatttttta tattatattt gtctgtaaac aaataaaagg tcacaagatt agcatgtaaa
    37921 tattttctaa aagtattcat tttgaaaagg aggagatatt ttggttataa aattcaacca
    37981 aaaagtaaga agaacattat cagtagcgat tctatttatg gtattaataa taggttgctt
    38041 tatagaaaag gatgacagtt tagaaacata caaagaaaaa actaattcaa aaataaatca
    38101 atataatatg gatgttatat ttgatgatga gactaagagg ttaatgtgca atcagaatat
    38161 tgagtacata aataatacaa aatcaaacat agataaaata tatttacata tatatccaaa
    38221 tgctttttcc aaaaaagatt ttgctccttt tgaaaaaagt gaaatgacaa gggcctatcc
    38281 taatggattt aacgaaggat atatagatat aaagaatata ttaaataaca atagtaaaat
    38341 ggagtataaa attaaagggg acaaaaatga catattagaa atagaattgg ggaaagagtt
    38401 aaaaccaaat gaaagaatgt cattagacat aaaatataac gtaaaaatac caaactcggt
    38461 tggaagattt ggatatggag aaaatactat aaatgttact aattggtttc ctatagtttg
    38521 tgtaaatgat gaaaggggat ggaatttaaa gagttatgaa actattggtg acccatttta
    38581 tagtgaaact agcgattttc atgtgaaact attgattcca aataaatata aaattgctca
    38641 tacaggaaaa cttgttaatg ataaacatga taataagaaa atgctatatg aaatagaagc
    38701 taaaaacgtt agagattttg catttatatt aagtagtaaa tttgatgtga ataaagtaga
    38761 ttcaaagggc gtagctatca atacatataa tttaaataaa aagctttcta aaaaagcaac
    38821 tgaagttgca aaagattcaa taaatatatt tagtgaatta tttgggaagt atccttacaa
    38881 agaattttct gttgtagcaa gtgacttttt tataggggga atggaatatc ctatgcttgt
    38941 tatgatagac caaagtttat ataatgaaaa aaatgaattt ttactagaat atgttattgc
    39001 tcatgagaca gcacatcaat ggtggtattc tgccgttgga aatgatgaaa taagtgaacc
    39061 ttggttagat gaagcactta ctgaatattc cacaatagtt tattttgaac aaaagtatgg
    39121 taaagatatg gcaaataagc ttataaaaac tatggagata cagacaaaaa gttatttgag
    39181 cgaaaatata tttaggccag cgaatgaata taaaaattct actgaataca gtttaaatgt
    39241 ttatacaaaa ggcgcaatag cgtttaatga aataagaaaa gaagttggag ataaagtatt
    39301 ctttgagact ttaaatgaat actataataa atataaacat aaaaatgcta atggaagagc
    39361 ttttgtggaa ctatggaata gtaaaggggt agatataaat aagattataa gtgaatgtaa
    39421 gtaaatttct tatagagtat taaattttat ttaatactct atttttttgg tataatataa
    39481 tttataaggc agaaaaagag gtgaaggaat gctaaagtat tgttcagtag ggagtggtag
    39541 tagtgggaat tgccactata taggatataa aaatactaat atactagtag atgcaggatt
    39601 aagtggcaag agaataacta caggtctcaa agacattggt atagatgcag ataaattaaa
    39661 aggaatattt ataacacatg agcatgttga tcatataaaa ggagcaggta tactatcaag
    39721 aaagtttgat ataccagtat ttgctaatat aaagacatgg tgttctatga aggacaaatt
    39781 aggtgatatc aaagataaaa atatgaaagt atttgaaaat gataaaacct attcattagg
    39841 tgatatcata gtgagacctt tttcaataga acatgatgcg tctgacccag ttggatataa
    39901 tttttataca gagaatgatg aaaaaatgtc tatagctact gatataggat gcataactca
    39961 aaatataaaa aaacatctat ataaatctaa gttagtagta ttggaatcaa attatgaccc
    40021 taacatgctt atgatgggtt catatacata tgctttgaag aaaagagtaa tgtcaaatgc
    40081 tggtcattta tcaaatgaag atgcagctaa tttctgtgtt gaacttatta atgaaggcac
    40141 agaatctata ttattagcac acttaagtaa agaaaataat tttcctgagt tagcatatga
    40201 gacctcaaaa ggagtattag catcaaatga tatagtagtt ggacaagatg taaaactaga
    40261 tgtactatca cgaaatgatg tttctaatgt ttatgagatg aagtagaggt gaataacttt
    40321 gctaaagaga aatttcgaaa acgttgaaga taataaaatt ttatactcta taaagcaggg
    40381 aatgatttta gcaatacctg ctattatgac tggttcaact gctcttgtaa ttttgaatct
    40441 tccaataaag gtttatcaag aatatttatc aagcttgttt aatggagagg ttacaaatat
    40501 tcttaacttt ataaatgatt ctactttagg aattatttca ttaataatat tattaacaat
    40561 aagctatagt tatgggaaaa tatatgggag caagtataca gtacttgttc ctatagttgc
    40621 aatgtgctct tttttagtat tttcacatgg taatgagctt gattcctata tagaaatatt
    40681 taagacaaag tggttattta catctataat tgtttctatg acatcttctg tattatttgt
    40741 aagacttaca gaatcttttg ttacaagtgt aaaatttcat acagaagggg ctgatgcaga
    40801 ctttaatatg gtagtttcag ctatagttcc tttcataata gtagtatttt tattctctat
    40861 ttttagagta ataatgataa gtctaattgg cagttcaaac tttcaagata tattttataa
    40921 tttattttca aatgtgttta atagaatggg aacaaatctt atgagtgctc tgttgtttat
    40981 ttttttaatg cactttatgt ggttttttgg gattcatgga ggtaatgtat tagatactgt
    41041 tgcaaaaaat ttatttgaaa atagcatggc aattaacata aatttagtta ataataatca
    41101 gttaccaaca gaaattttta ctaaaacttt ttttgataca atggttttat tggggggatg
    41161 tggcttttta ctatgtctag taattgcaat ttttcttagt gagaagcgta taaatgtaag
    41221 aaagttagct aaaattgctt ctataccagc tgtttttaat ataaatgaga tgctagtttt
    41281 tggaatacca attgttttta attatattat gtttgtacct tttgtaatta ctcctataat
    41341 tttaaccatt acaagttatg tggctatgtc tacaggcatt gtaccctgta cagtttcagc
    41401 tgtagaatgg acatcaccga tatttttaag tggatatata tctacaggtt caataagggg
    41461 tagtttgttg caattattta atctatgtgt tggtgttatg atttatattc catttataaa
    41521 gatatcgcaa aatagatata catatatgtt taaaaataat atagaaaatt taacaaagct
    41581 agtaaaaagg tatgaattaa caggggaaca gcctaatttt ctaaaccata atggtaaaat
    41641 aggctcaatt tcaaaaatgt tggcatcaga cttgaagttt gctatgaaaa gaggagaaat
    41701 agagattttt tatcaaccac aagtcaatta taatggagat gttgtagggg cagagggatt
    41761 acttagatgg aaacattcga taggagggtt catttatcca ccactagtta taattttggc
    41821 taaagaagaa ggttttctag atgaattagg agagtatatc ataaataaag catgtgttga
    41881 tttaaagaaa agtgaaaagt tattgagaaa ttctgttaag ttttccgtaa atatatctcc
    41941 agaccaactt gatgaaccta agttaccaga acaaataaaa aatatcatta gtagtaatga
    42001 tataaatcct aatatgctag gtatagaaat tacagagcag gttgcattat caggttcaca
    42061 aataattatt gaacgtatca atgcgataca taacttggga ataaagctaa ttatggatga
    42121 ttttgggatg ggacatagtt ctcttatata tcttcaaaat aataattttg atatagttaa
    42181 attggatggt tcattagtta aagagatact tacaaataaa agaagtagtg aaattataat
    42241 gactattgta aatctttcaa aaaatttaga ttttgatata gttgttgagt atgttgaaaa
    42301 tatggaccaa agagataagt tatataggct aggatgtgaa atatatcaag gatattatta
    42361 tagtaaagct ataccttttg aagagtttat agagtatgca aatcaagaaa gagagatata
    42421 attttataag agcttgtaaa ttctagtaag gtatagattt gtaataaaat taagtttagg
    42481 agaaatatat gaatataagt atagtagtag taggtaaaat taaagaaaaa tatttaaaat
    42541 taggtattga tgagtttaaa aaaagacttt ctaagtattg taagttagaa attatagagt
    42601 tggatgatga aaaagctcca gagaatttga gttctaagga aatggaaatt ataaaagata
    42661 aagaaggaaa gaaaatttta gggaaagtaa agcataatag ctatgttata gccttggcta
    42721 ttgatggaaa gagcttgtcc tctgaagaac ttgctaagac catgagtgat ttagcagtta
    42781 gaggtaatag tagtctatgt tttataatag gaggttctct agggctttct gatgaggtct
    42841 taggaagagc tgattatagg ttatcttttt ctaggatgac ttttcctcat cagatgatga
    42901 gattgatatt gctagaacag atttataggg cttataggat aaataatgga gaaccgtatc
    42961 ataaataaat gatacggttt ttgttagttt gccacttaat aatattaaaa taacaaaaaa
    43021 gtattgaaag tatattgtat ttaaggtaga attaaataaa atattataac attataatgt
    43081 ttagagggtt gttaataaaa atttggagga aaatatatga taaaaataat gttgtcctgt
    43141 tcagcaggaa tgtctacaag tttattagtt actaaaatgg aaattgcagc taaagaaaac
    43201 ggtatagagt cgcaaatttg ggcaatacca gaatcaacta tacaaaatga aatagaaaaa
    43261 tgtgacgtat tattactagg accacaagtt agatacgtat tacctaaggc tcaggaaatt
    43321 gctaagccat ataatatacc agtagaagtt ataaatatga tgcactatgg aacagtcaat
    43381 ggagaagctg ttttaaatag agctatagaa cttaacaaca ctaagtaata taaaatataa
    43441 tgtattttta atatatcaat tgggagaatc aaagaatgga tgaaaagatt atagaaatat
    43501 cttttaatat aataggttat gcaggtaaag gtaaaggatt agcctttgag gcaataaaag
    43561 aagccaagaa tggaaatata gaaaaagcaa aagatttatt aaaagagtct aaggaagtta
    43621 taaataaggc acataaatat caaacagaat taatacaaaa cgaagctagt ggaaacaaga
    43681 ctgaaataag tgttatactt atacatgcac aggaccactt aatgaatggt atgaattttc
    43741 aacagttggc tgaggaaatt gttgatttac atttaaagct acaagataaa taaaaattaa
    43801 agaaaataaa ttaagagagt tcttaaaata tacattatgt gtagcacact aattgttaga
    43861 ttattgttta atatttggtg agcaatacat taagtttatt ttgaggactc tttttgtatt
    43921 tagtttaaag ttaatagata tttattatta ataatataa
//
LOCUS       DAFNNB010000013        43382 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0013, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000013 DAFNNB010000000
VERSION     DAFNNB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 43382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43382
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0013"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            37..1164
                     /locus_tag="L3M60_000648"
     CDS             37..1164
                     /locus_tag="L3M60_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6225347.1"
                     /translation="MNNDKKVIKVHNKSDKPDNIVIKENELIEKCLLLENKLPVFMKD
                     YFIYLKGSVAVSTRLAYLEDINFFCSYLIETKELTNAECIKDITEDDFNTIKSRDINL
                     FLGDYCSRYYKNTEKNTLIFENNNRALARKKSSISTLFKFLYRNSQIDNNITDGFNPI
                     KLPKPQPDAIKRLEIDEVAKMLESVETGEGLTEKEKVYWRKTKLRDKAILALFVTYGL
                     RLNELRELNISSFNFSRGEFKIYRKRGKEVLMPINHTCEHVIKDYLQNERTRDELLND
                     EEKDALFLSLQNKRITAKAIRTLVKKYTSIPLDTTRENGYSPHKLRATAATSLIQTGF
                     SIYDVQNLLDHDNVTTTQLYAAHKKNVKRDIVKNFEWIEDD"
     gene            1268..1903
                     /gene="lexA"
                     /locus_tag="L3M60_000649"
     CDS             1268..1903
                     /gene="lexA"
                     /locus_tag="L3M60_000649"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HBN6225348.1"
                     /translation="MYLDLTEKQVLILEFIKSQIILKGYPPAVREICTAVGLRSTSTV
                     HSHLNKLEKLGYIRKDPTKPRAIEVLERSKVNDVSGANQEIIELPLVGQITAGEPILA
                     QQNIEEYIPFPASLVKGSNNFVLKVKGESMINAGILDEDYVVVDKKNTAFNSQIVVAL
                     INGESATVKRFFKEGNLIRLQPENDFMEPIMLNDSEVEIVGIVTGVFRVIK"
     gene            complement(2022..3179)
                     /locus_tag="L3M60_000650"
     CDS             complement(2022..3179)
                     /locus_tag="L3M60_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6225349.1"
                     /translation="MIERNIKNKKNSKIKALILLFIVSISIYLTGCATSDNGNNQSNN
                     NDSLSSSISSDKNVKIHFINTGNSDAILIQDGKTFTLIDGGDNDDEKLMVDYLNNQGV
                     KDIKYLIATHSHADHLGGLDSVVKNFNIENVFVSNGSAETKSYRDFINALASKDLSPS
                     VPLENNKFYLEDSYFEVLNTNGGDTTNEQSLVLVYTNGNDKVLFTGDAEEGTEKEILS
                     KLDKVDLLKVAHHGSRSSSSQEFLDKVSPEYAVLLVGKGNSYGHPHQETMNKLKKMGV
                     KVHRSDECSDIIFESTGDGVFTSCKDGSYNKGVREDGNSGKSNNNTSKNDSFKNKQNT
                     SNSAEIVYFTPKGKSYHSTKNCSGLSRSKKILSGTIAESKKNDPCDICYVK"
     gene            complement(3508..4140)
                     /locus_tag="L3M60_000651"
     CDS             complement(3508..4140)
                     /locus_tag="L3M60_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBN6225350.1"
                     /translation="MNNKYRRTVNREFEKSSFWPTFTDLLSTVLMVVILILFSSESIS
                     GSVEQDLAKNVNTSVEETFKKSGIPVKVDKSNGQVTFGERTMFDIDSDVLKPEAKEML
                     KMFVPKYMETIYKDYGDYISKIVIEGHTDDVGSYIYNLDLSQRRAYSVAKFIVGDEIG
                     DYKYKDKVTKHIIAIGRSKAELIKNGDNSVNRDASRRVELKYEININQNK"
     gene            complement(4133..5491)
                     /locus_tag="L3M60_000652"
     CDS             complement(4133..5491)
                     /locus_tag="L3M60_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225351.1"
                     /translation="MSNIITNLIESIFTNPITILFLLAITVYSVFKIIENNRVYSFVS
                     SKLDEINRDYKTSEFYKKIRDDYYTYSKENPYADVNITSFIEEVVSDLKHNNLPLLEK
                     IRSIKNSSSISILLGVLGTFVGLSTMLLCVDTKDIINSLPSTISSMQTAFTTSIFGVV
                     FSLIIGYFTKIKDCEHVLIQIMLKSENLLTSEITHFKSERIDLKVEEVKNTIKQISKS
                     IEAIEKFDKISKDLNDFNDEFISGIEALKSLLEGSQSSIKTFDQSVRKLDKQFNILNI
                     KFVKLFDKYDNQDNINKEILFDIKESSKNIYNATESQFKIRDYIKNINAGFALYERSA
                     QDLLTKLMTHESKISQNQKILLDEKFTLDDSIKNLSNIIENFSNDLQVKLDMMFENSL
                     DIQDKLDVMFNNSFMNDEVPLDSEELFNDDVNNVFNPFSEEIYEIEDKEIKVIGEDEL
                     NE"
     gene            5661..6260
                     /locus_tag="L3M60_000653"
     CDS             5661..6260
                     /locus_tag="L3M60_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6225352.1"
                     /translation="MNDNIVEVKNINMNFYTKEGELEVLKDVNFNLKEGEILTLLGPS
                     GSGKSTILNILTNLLKPTSGDIKITGNIGYMFQKDNLLEWRNIMDNITIGLEIQGKKD
                     KKSLDRVESLLKTYGLWDFRSMYPKELSGGMRQRVALIRTLSVNPDILLLDEPFSALD
                     YQTRLLVCDDVYSIIKNENKSTILVTHDIGDAISIKVQL"
     gene            complement(6407..8008)
                     /locus_tag="L3M60_000654"
     CDS             complement(6407..8008)
                     /locus_tag="L3M60_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HBN6225353.1"
                     /translation="MSNITNSIKDFRMEINQDFIDKAEKLKPILIEKLIKPKQIVNIK
                     MGKNDWEVIDTKSIDEISNISLGKGESICIDFGNHQVGYVNFKIKPIGSPPDAPTHLR
                     IKLGETLCEIGEDSSTYKGSISSSWIQEETMHIDILPDKINMKRRYAFRYLEIKVLDT
                     SPKYKVIVEDVSCNFVSSVDMSEINYLSNIDDDLIKIDKIALKTMQDCMQTVFEDGPK
                     RDRRLWIGDLRLQAITNYETFKNNDLVKRCLYLFAGLTQNRGQVGACLFIEPKLLVDD
                     TALFDYSLFFISCLHDYYEATKDMEILNELWKVAYIQAKLALERVGINGVVYDSDDWW
                     CFLDWKEGLNKQAGAQGVLIYALKQAHKLAIILGDNEKKEELEYEIDRVVQGAKNHLW
                     DEKQGFFVSGSEKQVSWISQIWCVLADVFDKETNTKILDNLLDNPPDMDIVTPYAYHH
                     LVECLILNNRKEKALECIRGYWGEMVKDGADCFWELYNPNDKYVSPYGSRIINSYCHA
                     WSCTPSYFIRKYFGRDSKEVTSKYK"
     gene            complement(8039..9406)
                     /locus_tag="L3M60_000655"
     CDS             complement(8039..9406)
                     /locus_tag="L3M60_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225354.1"
                     /translation="MTTLKVVDEKNVPGIWRQRIGYGSSDLACNLIWQMISLYLLYFY
                     TDIMGLSAVAISFMFVVTRVIDGITDLLVGYCIDKTNTRWGKSRPYFLFGAVPFALFA
                     VLAFSVPDISLSGKLIYAYVTYIGLSLTYTIVNIPMASILPSLTDDTNERTALSTSRK
                     FFGFLGSTIVSYSALTLVGKFGGTDEALGFKVVMMLFAFIGCAIFLFTFSNVRERVEI
                     KSENVTLFQTIKSLKENKPWKLFALNILFMWTGFFIQSSALIYYFSYNVGSKSLATTV
                     ATIMSIIPILANFLVPILAKRLGKRNLYITSAAVQFIGLGIILIANLNISLIIAGSIV
                     SAFGYGIKESIYFSMQADPVDYGIWKTGINTTGTLNSVNGFLGKCAQAISGGLGGALL
                     AWGGYIAKAEVQPASALVAIKIMYVYIPMALLICSMITMKFYDLDKQYPKIKADLDAR
                     KISEE"
     gene            9579..10628
                     /locus_tag="L3M60_000656"
     CDS             9579..10628
                     /locus_tag="L3M60_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6225355.1"
                     /translation="MSLNKLFQNLYIYNESELFYKNYYFAKQNPESLKLFIDNLDEDY
                     IVNNNLIIPYIYNEKIPQLMKDETYFECNSKDNVILIKHNRYTPQFVHEHIFFEMIYV
                     LSGKCYQVINHHNVHMKEGDICIVSPGVKHSIGVFDDSIIINVLIRRSTFEDAFFDFL
                     RNNNLLSSFFLNSIYKDKQNDYILFHTNNDKSIEISILEMLSEYNEQDRYYNNILNHL
                     IMVFFAKLLRDYEDSIEVPNVTKKGTLNHTNILTYIQDNYVDITLEELANKFHFSIPY
                     CSKIIKEIAGCNFTQLLQRIRLERAESLLMNTNIPIADISNNVGYPNVEHFIRLFNKK
                     YNMSPSKYRKNTTKV"
     gene            complement(10748..11221)
                     /locus_tag="L3M60_000657"
     CDS             complement(10748..11221)
                     /locus_tag="L3M60_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF861 domain-containing protein"
                     /protein_id="HBN6225356.1"
                     /translation="MDISNIDKNLIETLVRQIIEEKISGTKDTVDFVRNKDISGITSI
                     KLPTVKVSESDRLDTGNPSDVVYTKDLFTLEESPRLGCGMMEMKETTFDWTLNYDEID
                     YVIDGTLDIIIDGRKVSASSGELIFIPKGSKIQFSVPDYARFIYVTYPADWASQN"
     gene            complement(11223..12314)
                     /gene="eutH"
                     /locus_tag="L3M60_000658"
     CDS             complement(11223..12314)
                     /gene="eutH"
                     /locus_tag="L3M60_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBN6225357.1"
                     /translation="MSINEIIIYVMVIFMVLGAIDKCIGNKFGLGEQFEEGIMAMGSL
                     AVAMVGVICLAPVLADVLRPIVVPVFNILGADPAMFAGSLLANDMGGAPLALELAKDP
                     NAGLFGGLIVGAMMGPTIVFIIPVALGIIEKEDQKFLATGILAGIITIPIGAFVGGLV
                     AGFEIMMVLRNLVPIIIFAVVIALGLLKFENAMIKGFTYFGKGVVIVITLGFAAAIVE
                     ALTGIVVIPGMKPISEGIEIVGDIAIVLAGAFPLVFVITKVFNKPLMALGKVLGMNDV
                     SAAGLVASLANCIPMFGMMKDMDNRGKIINVAFSVSAAFVFGDHLGFTAGFNSNMITP
                     MIVAKLVGGITAVLLAMVIANKTLKKENV"
     gene            complement(12326..12877)
                     /locus_tag="L3M60_000659"
     CDS             complement(12326..12877)
                     /locus_tag="L3M60_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBN6225358.1"
                     /translation="MNKSIGAIEFKSIAKGIEVSNEMIKKSSVDVLYLKSICPGKFLI
                     VVGGETSYVNECVDYGIKLGEGYIVDNFVINAISQEILDGFKNKYQKLDNIVSIGVVE
                     SGKVCAGIKMLDRTLKSGELVLVKLQLSFAIGGKLVYIVAGDLSSLEYALKESEDVVR
                     EKEVIYKTVIPSVDSQIIKSLIK"
     gene            complement(12870..13142)
                     /locus_tag="L3M60_000660"
     CDS             complement(12870..13142)
                     /locus_tag="L3M60_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HBN6225359.1"
                     /translation="MDIGKVVGNVWATRKDEKLCGQKLLVVKILKTKGTYKEGFMVAA
                     DNAGAGNGDLVLISRGSSARESIEKAHVPVDATIVGVVDSFEVFDE"
     gene            complement(13155..13880)
                     /locus_tag="L3M60_000661"
     CDS             complement(13155..13880)
                     /locus_tag="L3M60_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02536 family ethanolamine utilization
                     protein"
                     /protein_id="HBN6225360.1"
                     /translation="MNYDNLVDLIMEEIYKKVNSNEIKISNKPKAVIVFEQDTNKFNL
                     LKDEFEIVEFDKSIKECEIVIVSRLCMRGLSNIALGNSTSDEERFILKMIMSGKKVYV
                     LDEGIEYKKYKDTAPKALYKKFISFEDEICKFGVEIIKDLNAITKNKLNIKRELESTI
                     GSKKEDTKALDKDVLNLTSVESVKIDNEFSLDLRNRKLISEADLRKPTINGVKNILVN
                     KKSIITPLAVDFMRIHHLKLKKV"
     gene            complement(13922..14551)
                     /locus_tag="L3M60_000662"
     CDS             complement(13922..14551)
                     /locus_tag="L3M60_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBN6225361.1"
                     /translation="MENLLNEIVEEVITRVKKEAFIEVEASGRHVHLSREDVDKLFGK
                     GYTLTKLKDLSQPGQYACKERVTITGPKGSIKNVVVLGPCRNETQVEISLTDGLTLGL
                     KAPIKQSGDLEGTLSIKISTQHGEVELDKGLMVAKRHIHMTPKDAENFNVCDKEIVEA
                     KVMGQRPLTFDDVVIRVSDSFKTYMHIDYDEANACGYSKGTVAKIIKKV"
     gene            complement(14564..15325)
                     /locus_tag="L3M60_000663"
     CDS             complement(14564..15325)
                     /locus_tag="L3M60_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin adenosyltransferase"
                     /protein_id="HBN6225362.1"
                     /translation="MGVLTENEIKRKIGKNKNNITEFYIEKGQIITPSAKSYLAERSI
                     ELKYVDKLSDVNNEPKEVVKEVVKEKTYKYTTVFGAKLDEKPEHMTQLNGNLLVFKDH
                     KRIILRGKIDSLESEILKAQILCQKLDMPKLVKELQEVLDFVRNIMRCEVLEEKLEDF
                     KLLGLTPQELREQSHYPKKYFGIGHEFPSYEMGEVVVAINSIRSNTREVELAAYEAFK
                     GEYGNVHREDLIKALNRLSSVFWIMIYKVRTNKYK"
     gene            complement(15452..15739)
                     /gene="eutM"
                     /locus_tag="L3M60_000664"
     CDS             complement(15452..15739)
                     /gene="eutM"
                     /locus_tag="L3M60_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBN6225363.1"
                     /translation="MASANALGMIETKGLVGAIEAADAMVKAANVQLVGKEQVGGGLV
                     TVMVRGDVGAVKAATDAGAAAAERVGELISVHVIPRPHFEVDAILPKVSAE"
     gene            complement(15825..17294)
                     /locus_tag="L3M60_000665"
     CDS             complement(15825..17294)
                     /locus_tag="L3M60_000665"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase (acetylating)"
                     /protein_id="HBN6225364.1"
                     /translation="MTLLDKDLVSIQEVRTLVRRSKVAQDKLSKMSQEQIDKIVKAIA
                     DAAFNNATKLAKMANEETGFGKWEDKVIKNTFASMGVYETLKNTKTIGIISEDKEANT
                     IDIATPVGVVAGLIPSTNPTSTVIYKTIISLKAGNTIVFSPHPNAKNCILETVNIISD
                     AAIKAGAPEDSILCITNPSIVGTNELMKHKDTNLILATGGEAMVRAAYSSGTPAIGVG
                     PGNGPAFIDKSANVKLAIKRIIDSKTFDNGVICASEQSIVIEKSMEETVLKELKNQGA
                     YVLNEEESKKLAKFILRANGTMNPQIVGKSVEVIANLADLTNVPKNTKVLVARENKVG
                     HNVAYSREKLTPILAFYVEENVEAIMNRCREILLNEGAGHTFSMHANDEELVKRFALN
                     MPVSRILVNTPSALGGIGASTNLVPALTLGCGAIGGSSTSNNIGPLDLINVKRVAYGI
                     KEIEELGRDKSQVEKSMFDSIDKEELINLLVQKLVKELG"
     gene            complement(17297..18001)
                     /locus_tag="L3M60_000666"
     CDS             complement(17297..18001)
                     /locus_tag="L3M60_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBN6225365.1"
                     /translation="MDAIGLIEVIGYVAAIEASDACLKSANVNIVRIDKVGAGIVTLT
                     ICGDVGAVKSALEAGEIAASRVGTLRTSHIIPRIHDEVTDALFKTKEPKVCEVSEDKE
                     KNFEVTLETTDNVSKNLSEKEDVILDVTENSGNIEKLYSDIDKINKEIETVDDILTEA
                     NKGKIENTNIDKKVLEQVKIENFDFDRDDLSKNSVKKLKAMAKKLDSSITYKDLNVLK
                     KEELINLVKKLSRRDR"
     gene            complement(18012..18665)
                     /gene="eutL"
                     /locus_tag="L3M60_000667"
     CDS             complement(18012..18665)
                     /gene="eutL"
                     /locus_tag="L3M60_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBN6225366.1"
                     /translation="MKNDVIRPNILGIKIISNISPEMAQKLELKSHHKSLGLITADCD
                     DVTYTALDEATKASEVDVVYAKSMYAGAANASTKLAGEVLGIIAGPSPAEVRSGLNAV
                     VDFLEYGATFISANDDDSIAYYAHCVSRTGTYLSEVAGIREGEALAYLVAPPLEAMYA
                     LDAAMKAADVKMCELFAPPTETNFGGALLTGSQSACKAACDAFAEAVKSVADNPTGF"
     gene            complement(18685..19566)
                     /gene="eutC"
                     /locus_tag="L3M60_000668"
     CDS             complement(18685..19566)
                     /gene="eutC"
                     /locus_tag="L3M60_000668"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014952556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HBN6225367.1"
                     /translation="MNEKDLKALVEQLVGQMVGELDTNVVSETVKKATEVVVDNNACI
                     DDITEVDIRKQLLVKNPKDAEAYLDMKAKTPARLGIGRTGTRYKTETVLRFRADHAAA
                     QDAVFSYVDEEFIKENNMFAVETLCKDKDEYLTRPDLGRKFSPETINNIKSKFGTNQK
                     VLILVGDGLSSAAIEANLKDCVPAIKQGLKMYGIDSSEILFVKHCRVGAMDHLGEELG
                     CEVICMLVGERPGLVTAESMSAYIAYKPYIGMAEAKRTVISNIHKGGTTAVEAGAHIA
                     ELIKTMLDKKASGIDLK"
     gene            complement(19579..20943)
                     /locus_tag="L3M60_000669"
     CDS             complement(19579..20943)
                     /locus_tag="L3M60_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HBN6225368.1"
                     /translation="MILKTKLFGRTYQFKGLYDVMAKANEEKSGDKLAGLAAESAEER
                     IAAKVVLSHIKLEDIRNNPAVAYEEDEVTRIIQDAVNEKVYEEIKNWTVSELREWILD
                     SNTTSLDIRRISRGLTSEMIAAVAKLMSNMDLVYAAKKIRVTAHCNTTIGEQGTLSAR
                     LQPNHPTDDPDGILASLLEGLTFGIGDAVLGLNPVDDSVESVTKVLKRFEEVKQKYQI
                     PTQTCVLAHVTTQMEAIRNGAPADLIFQSIAGSEKGNEAFGFNGDTIEEARQLALKYG
                     TATGPNVMYFETGQGSELSSEAHHGIDQVTMEARCYGFAKKYEPFLVNTVVGFIGPEY
                     LYDAREVVRAGLEDHFMGKLHGLPMGVDVCYTNHMKADQNDMENLAILLTTAGCTYFM
                     GIPHGDDVMLNYQTTGYHETASLREMFGLTAIKEFDAWMEKMGFSKDGKLTELAGDAS
                     VLLG"
     gene            complement(20961..22394)
                     /gene="eutA"
                     /locus_tag="L3M60_000670"
     CDS             complement(20961..22394)
                     /gene="eutA"
                     /locus_tag="L3M60_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004458698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBN6225369.1"
                     /translation="MREELLSVGIDIGTSTTQLIFSKLIVENTESNFSVPRISIVDKE
                     IIYKSKVYFTPLISNNEINGQQIKDIVENEYKKANISKVDIHTGAVIITGETARKENA
                     NDVLHTLSGFAGDFVVATAGPDLESIISGKGAGAHIYSKEHSTTIVNIDIGGGTSNIA
                     SFKRGEVIDTGCLDIGGRLIKIDKISKKITYISPKIDKIIKDNNLNLQVGTIATIENT
                     KPIVDIMVDLLMESIGLKKQTPLFDFIITNKSISVDENIKNVSFSGGVADYIYYDGEI
                     KDYFEYGDLGILLGQAIKNCKYLKKVNVVKSMETIRATVVGAGSHTTEVSGSTITYTS
                     EKFPLKNLPVLKLSIDEETGTSKQISEAIKNKLNWFKLENDLQKVAIAMIGKKNPTFL
                     DIQEYAKGFVDGMQQIIENDLPFIVIVENDMAKVLGQAIYALLNYKKEIVCIDSVKVE
                     NGDYIDIGKPIVEGKVLPVVIKTLVFN"
     gene            complement(22578..23987)
                     /locus_tag="L3M60_000671"
     CDS             complement(22578..23987)
                     /locus_tag="L3M60_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal domain-containing
                     protein"
                     /protein_id="HBN6225370.1"
                     /translation="MTNTIKKISTAQTYLNNNEIEKIIEVSKSLDLMASFYEADVFID
                     ILSKIDDEAIVVAHGRPKEKSMYCENVIGKKALIENEPGVIKCLKTGERVRDIKALTQ
                     EYKLVKQNIQPITLDDKVLAVLIVEKDISSEVKNEFNASEDEENKNLHQFIKLIGNND
                     FLVDNLNSAILIFDKNGNLKIKNRIATEIYKNLGFGYDIQDMHYNDLDIETNTFEDIL
                     MDENYDETNEVSIDNMFFKIKTIYIKDDEFKVCKIVQDISDLKRKEEELVLKTVAIRE
                     AHHRVKNNLHTVISIIKKQSRLSQNDEVKQCLDNIINRVFAILSTHYLLSKEVDNNIS
                     IREGINLLVSNIQGSYLFSKDIDIRITGEDFKISGEKATAILLVINEILQNCYDHAFL
                     DREHGTIQILINKDNNYRNIAIIDDGVGFDSDNINKNSLGTYIIDSYITQMLKGNIER
                     KSNKNGTEILITIPTQINI"
     gene            complement(23980..24555)
                     /locus_tag="L3M60_000672"
     CDS             complement(23980..24555)
                     /locus_tag="L3M60_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBN6225371.1"
                     /translation="MGKKILLVDDEPLLRMDTKDILESHGYEIIGEASDGFEAVEMCK
                     KHKPDLVIMDIEMPILDGIKAGKIICKENLAGGVLLLTSFEDKEYVEKAKSIGAYGYL
                     VKTASEKSLIPTIEMCLNKVNEFEKMKKDLEKVNSKLNERKLVERAKGILIREFKISE
                     DEAYTRIRKLSMDKRTTMMEIAKMIIIGYDD"
     gene            complement(24658..25107)
                     /locus_tag="L3M60_000673"
     CDS             complement(24658..25107)
                     /locus_tag="L3M60_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBN6225372.1"
                     /translation="MKNIIFMGKTGSGKTTLCQKLDQLELNYKKTQSVELYNNAIDTP
                     GEYMENRNLYNALITTSADAKIIAIVYDATQEENYIAPGFASMFCKDVIGIITKINKA
                     TENEVNIAYERLSLAGASQIFKIDTVDNIGLEKLFKYLEKSKNIDEV"
     gene            complement(25115..25465)
                     /locus_tag="L3M60_000674"
     CDS             complement(25115..25465)
                     /locus_tag="L3M60_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBN6225373.1"
                     /translation="MTEESKQRVIQEYVPGKQVTLAHIIANPNEDIYKKLGLVLDKKD
                     AIGILTITPSEASIIAADVATKASNVSLGFIDRFSGSVVISGDVSSVESALNDVLEVL
                     GNMLNFSSTKITRT"
     gene            complement(25706..26836)
                     /locus_tag="L3M60_000675"
     CDS             complement(25706..26836)
                     /locus_tag="L3M60_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBN6225374.1"
                     /translation="MNNFEIKTRIKFGEGSLKALQELKNKNVLIITDPFMVKSKTIDK
                     ITSNLVNSTYQVFEEIVPDPPIEVVVSGIEAFKSINPDVIIALGGGSAIDAAKSIMDF
                     SKKILKINEVEFIAIPTTSGTGSEVTSFAVITDSQKKCKYPLVSDDLLPDMAILDPEL
                     VKTVPNFITADTGMDVLTHAIEAYVSTNSNDFSDALAEKAIKLVFKYLIKAYKDGNDI
                     EAREKMHNASCLAGMAFNQASLGINHSIAHVLGGKFHIPHGRTNSILLPHVIEYNSSI
                     VGYTNTTYSDTAKKYAQIAKLVGLGNANIRISVRNLVNEIKKLQKEMNMPTKLTQCKL
                     SLEDITNEEREIAKLAIKDTCTLTNPKVPTEDDIINILNNIK"
     gene            complement(27010..27186)
                     /locus_tag="L3M60_000676"
     CDS             complement(27010..27186)
                     /locus_tag="L3M60_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225375.1"
                     /translation="MEINVIEIFPKDKAKLNEIEMNKASWFVNIISKKYPKEALNEAF
                     STLEKELNISKANT"
     gene            27514..29112
                     /locus_tag="L3M60_000677"
     CDS             27514..29112
                     /locus_tag="L3M60_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN6225376.1"
                     /translation="MKAAIYSRKSRFTGKGDSIENQIQLCIDYAKSIGIKDFLIYEDE
                     GFSGSNIDRPQFKKMMQDAKDKKFNYLVCYRLDRISRNVSDFSTLIEKLNELNISFIS
                     IKEQFDTSTPMGRAMMFISSVFAQLERETIAERIKDNMYELARSGRWLGGNTPHGFNS
                     EKITFLDENLKERSMYKLQVNDEQMEVVKLIYNKYLELRSLSKLYKYIYNNGIRGPRG
                     GKFDPSSLSRILRNPAYVKANDEILEYLKNNGMDIVGTPDKKCGVLTYAKNTTNSIAA
                     IAKHHGVIEPEIWLEVQNQLDKNRDKTPRIATGKTALLSGFIKCGKCGSNMRITYKGR
                     KTDKDLKYYYICGTKKTLGADNCNCKNLNGPLVESLIIDKIKNCKEESIISTFNNNNI
                     KTGNIFNVLKSETNLLKNQILDKEKLIKNLVIELAKNTDSIASDYIVSQIENLNSEIN
                     ILKDKLNTLESSNANISSDSINLDIIVSNLKRFNDEVDNASLDDKRLLLSTIINSIVW
                     DSNLGNLNIIYMGAEMDNSVSLSN"
     gene            29307..30107
                     /locus_tag="L3M60_000678"
     CDS             29307..30107
                     /locus_tag="L3M60_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225377.1"
                     /translation="MRLNKRSEGYINYVKGVKREKRNVLFYQLLILIGFIVIWELLAD
                     LNVINTFLFSKPSDIYNLFIQYASSGQLFKHIGISVYETVLGLVIGTVLGILVAIALW
                     WSEKLSKILDPFLVVLNALPKTALAPIIIVWVGAGIEGIVVTAVTISVVVTILSAYNY
                     FMNIDEEKIKMLKSFGASKSQILFKLILPANTGNLINLTKINIGMAWVGVIVGEFLVS
                     RYGIGYLIVYGSQVFKLDLVMMGVFVLAICAWAMYAVVNIIEKIYNSR"
     gene            complement(30365..31188)
                     /locus_tag="L3M60_000679"
                     /pseudo
     CDS             complement(30365..31188)
                     /locus_tag="L3M60_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897377.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
     gene            complement(31257..31763)
                     /locus_tag="L3M60_000680"
     CDS             complement(31257..31763)
                     /locus_tag="L3M60_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6225378.1"
                     /translation="MELIEWNYAYITDLVEFANDKEIAKNLRDNFPNPYTEQNARDFI
                     SFCLATPDVKQLSYAIFYQERAIGSISLTFEEDTSINKNAVLGYWLARNYWGKGITTN
                     AVKEICKIAFEKYNLNKIHANVYSYNRRSSKVLEKCGFKIEEYCHNSIIKNRNMIDCH
                     VYSLIRTQ"
     gene            complement(31777..32703)
                     /locus_tag="L3M60_000681"
     CDS             complement(31777..32703)
                     /locus_tag="L3M60_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225379.1"
                     /translation="MSDILSRISNKIGDKNIVDKLSALSKSDLNSLLLEVFDRQANTL
                     TATDILKSYQLNRFTIPSSIDPEEIHTLESKLLRKAFNMDIKTIMLSPSAPLGSCSVF
                     GSVDQYNVVSALRGTETLADPSNMLAIIIADKLKRKEENNTIPIHMATTARAIRAQNF
                     VGKGLYSHFGLYCMVSSGIDTGSYTCEKMLLEKHLNYYKDILSEIENVRFSIILRKRN
                     GYKDNDGFFDRMVETIKLIFPNIELSIHNDDVDNNYYKGINFKLSVKQGNDTVEMVDG
                     GFVDWTYQMLGNKKERCLISGIGLERFLVAVS"
     gene            complement(32828..33610)
                     /locus_tag="L3M60_000682"
     CDS             complement(32828..33610)
                     /locus_tag="L3M60_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6-adenylyltransferase"
                     /protein_id="HBN6225380.1"
                     /translation="MTKTQQEMIQNVLEFSKRDSRIIGLAISGSYITKSLDEYSDLDF
                     LIVADDKSYEEVIEERLLIVEQFGTLVSAFTGEHVGEPRLIISLYDSPVLHVDFKFTT
                     LDGLLDRVEDSAILYEENNCITEIYSKKEAYFPTPDLQWIEDRFWIWVHYACTKIGRR
                     ELFETIDFLSFLRQSVLAPLIQLLLGKLPRGVRKIELDAPQYIEALEKTVATHEYRSC
                     VNALQATINLYLELREELSQSPIVRKVEAERVALQYFQDLIK"
     gene            complement(33766..34386)
                     /locus_tag="L3M60_000683"
     CDS             complement(33766..34386)
                     /locus_tag="L3M60_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBN6225381.1"
                     /translation="MHLIDFMNEDIKKKLVKKTYKKKETILFAEQENEYVILMIDGIA
                     EAFILNAKGNISTIHLYRSGSFFGEMEQFNDGKKPVEIVAFTDCNVYKLHRDDFLNWL
                     KGDFEATKFLIREISSKLVVNAELIEELSSLSVKERLLKCISLHYYRSELNLLTKKQL
                     ASEVNAPIRSINRAINECVQEHLIAYENKRFSVINQREVLKNLPTT"
     gene            complement(35144..35680)
                     /locus_tag="L3M60_000684"
     CDS             complement(35144..35680)
                     /locus_tag="L3M60_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBN6225382.1"
                     /translation="MQGGVDIRQVKSFVFPVISLIFLDQISKVLIGLFLMGFEIDITG
                     KFLRFNPVQNTNLSYGGNFIGILSNLWVLVLFNILVILVIISGYAFYKSKNEQTSYSV
                     KVIMSCGLAGTICSLIDKLFWGGSLDFLQIPSFFIFDLKDCYLTVAEIIFVIIGILHS
                     REISMKEYIYFCYRQFKR"
     gene            complement(36071..36580)
                     /locus_tag="L3M60_000685"
     CDS             complement(36071..36580)
                     /locus_tag="L3M60_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225383.1"
                     /translation="MLSENIKTIRKSKGLSQQELAIKLNVVRQTISKWEQGLSVPDSD
                     MLISISEVLETPVSTLLGETIVELEVDNLKVISEKLEIINLQLVQRKNMRRKMFHWLF
                     ILLCSVIVIVFMVLGGLNSPYLNWNYTNPETAVFGVAFHAFECLFVRLTPIVLIGAII
                     GSFLTRKKI"
     gene            complement(36857..37006)
                     /locus_tag="L3M60_000686"
     CDS             complement(36857..37006)
                     /locus_tag="L3M60_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225384.1"
                     /translation="MNVNDNLKSINRELCVKTYDNFKILHDKEINRLKELKLNGYKLI
                     SSMKI"
     gene            complement(37027..37113)
                     /locus_tag="L3M60_000687"
                     /pseudo
     CDS             complement(37027..37113)
                     /locus_tag="L3M60_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606463.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            37993..38430
                     /locus_tag="L3M60_000688"
     CDS             37993..38430
                     /locus_tag="L3M60_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HBN6225385.1"
                     /translation="MDKKLENRCSWQEYFMRLCETVAERGTCDRAYVGAIIVNSENRI
                     VSTGYNGSISGDKHCSEVGHEMRDGHCIRTIHAEQNALYYCAKEGISVKDCSIYVTHF
                     PCLNCTKAIIQSGIKHIYYRTGYRIDEYAIKLLQSSNVLYTKL"
     gene            38569..39357
                     /locus_tag="L3M60_000689"
     CDS             38569..39357
                     /locus_tag="L3M60_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6225386.1"
                     /translation="MKVVIIPGHTLTGKGTGAVGYINESKETRILNDLIVKWLKIGGA
                     TVYTGRVDESSNHLADQCAIANKQETDLAVQIHFNSNVTTSTPGGTETIYKTNNEKTY
                     AERVNTRLATVFKNRGAKSDVRGLYWLNHTIAPAILIEVCFVDSKADTDYYVNNKDKV
                     AKLIAEGILNKSISNSQGGGVNKVYENVIVYTGDADKVAAQILHWQLKDSLIIEASSY
                     KQGLGKKVYVVGGEANKLVKGDVVINGADRYETVKLALKQIDKL"
     gene            39405..39863
                     /locus_tag="L3M60_000690"
     CDS             39405..39863
                     /locus_tag="L3M60_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225387.1"
                     /translation="MIRTKVVELIATVCRENKPHKWVDENYTPYDKSGKVELMSIEDL
                     NELISSNGKADLLYSCRLQKILKEIYINQSRASYMSGCGLFWSSYWDILEEKFEEWLY
                     NSYIFFDEDDEYLEGMEDFELECKDVLMDVIETTSIDIYVQMIKRNITNY"
     gene            complement(39963..40313)
                     /locus_tag="L3M60_000691"
     CDS             complement(39963..40313)
                     /locus_tag="L3M60_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225388.1"
                     /translation="MNNTITINISIIYLLLAILACIALVYLIIVLVKLHKILNTVGTM
                     LNENTQNINTTLNNLPSVVENFGEVGENMKDVTEVVTEVTADFLVTKDSVKSNMDVIV
                     DILTIIKNIFLDKK"
     gene            complement(40315..40698)
                     /locus_tag="L3M60_000692"
     CDS             complement(40315..40698)
                     /locus_tag="L3M60_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBN6225389.1"
                     /translation="MNLSKKIEDKRKAKQRAEKVKKAKIATAGVVLGAVTGAVSGVLL
                     APKSGKETREDIKDASQQIAEKINMKTVDVKGKVSEKLENKKGNFIESKKKIKKYIDN
                     KKSVENSSEEDKIIDIVEPVSVTEE"
     gene            complement(41204..41587)
                     /locus_tag="L3M60_000693"
     CDS             complement(41204..41587)
                     /locus_tag="L3M60_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225390.1"
                     /translation="MSTDKVVGIIDEETAELAGIEYTGKIYASSGVIKHIKKKHRCQL
                     SKDIFNDIIDTIKMVLKSPEYIGSHPKKPGKSVEFIKKIDDYILVATELDTKKEYLYV
                     SSMYAIKEAKLESRINSGRVKQYDG"
     gene            41936..42427
                     /locus_tag="L3M60_000694"
     CDS             41936..42427
                     /locus_tag="L3M60_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBN6225391.1"
                     /translation="MNNQQQEVYKKLDELNIPYEAVNHPPVYTIEEMEELKTLHMEFV
                     VKNLFLRDAKGKEHFLVVLGKDKKADLKDIRQQIDSKPLSFASEERLQKYLKLTKGAV
                     TPFGILNDKEAVVKVVFDKDLLKMDKIGIHPNDNTATVFLKFEDMKKLIEENGNEIYY
                     VEV"
     gene            complement(42552..43289)
                     /locus_tag="L3M60_000695"
     CDS             complement(42552..43289)
                     /locus_tag="L3M60_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN6225392.1"
                     /translation="MKQNKYDDDKFFNKYSKMERSINGLAGAGEWHTFRKMLPNFKGK
                     RVLDLGCGFGWHCQHAVENGATSAVGIDISEKMLKEARNKTTFDNIKYICMPIEDINF
                     PKDSFDVVISSLAFHYIQSFEDICKNISNCLSNKGDFVFSVEHPIFTAHGSQEWHCDK
                     DGNNLHWPVDRYFEEGLRKSNFLGEEVIKYHKTLTTYLNTLIKTGFEVLEIIEPQPEE
                     KLLDAIPGMKDELRRPMMLLVSAKKDK"
BASE COUNT        14241 a         7032 c         5401 g        16708 t
ORIGIN      
        1 taaaagattt atattttagt tttaggaggt ttaattttga ataatgataa gaaagttata
       61 aaagtacata ataaatcaga taaacctgat aatattgtta tcaaggaaaa tgaactcata
      121 gaaaaatgtc tacttttgga aaataaactt cctgtattta tgaaggatta ttttatatat
      181 ctaaaaggct ctgttgcagt ttctacacga cttgcttatt tagaagatat taattttttt
      241 tgttcatatt tgattgaaac caaagaatta acaaatgcag aatgtataaa agatattaca
      301 gaagatgact ttaatacaat aaaatcaaga gatataaatt tattcttggg tgattattgt
      361 agtagatatt ataaaaatac agaaaaaaac acattgattt ttgaaaataa caatagagct
      421 ttggcaagga aaaaatcatc catctctact ctatttaaat tcttatacag gaactctcag
      481 atagataata atataacgga tggattcaat ccaataaagc ttcctaaacc tcagccagat
      541 gccattaagc gtcttgagat agacgaagtt gctaagatgc ttgaatcagt agaaacaggt
      601 gaagggctta cagaaaaaga aaaggtatat tggagaaaaa ctaagttacg tgataaagct
      661 atactagcat tatttgttac atatggactt agattaaatg aacttagaga actcaatatc
      721 tcctctttta atttttcaag aggtgaattt aaaatatata gaaaacgcgg aaaagaagtt
      781 ttaatgccta taaaccatac ttgtgaacat gtaataaaag attatttgca aaatgaaaga
      841 acaagagatg aactattaaa tgatgaagaa aaagatgctt tatttttatc tcttcaaaat
      901 aagagaatta cagcaaaggc tataagaaca ctagtaaaaa aatatacatc tattccactt
      961 gatactacga gagaaaatgg atatagtcct cataaattaa gggcaacagc tgcgacttct
     1021 ctaatccaaa caggattttc tatatatgat gttcaaaact tacttgacca tgataatgtc
     1081 acaactactc aactttatgc agctcacaag aaaaatgtaa aacgtgatat agtcaagaat
     1141 tttgaatgga ttgaggatga ttaaaaatca ggaacaaatg tttgcagata taaatggatt
     1201 atgttataat taaaaatata aagtgtatgg aaataaaaac aaaatatgtt tggaaatgag
     1261 tgatgttatg tatttagatc taactgaaaa gcaagtattg atattggaat ttataaagtc
     1321 tcaaataata ttaaaaggtt atccacctgc tgtaagagaa atatgtactg ctgtagggtt
     1381 aagatctact tcaactgtgc actctcatct aaataaactt gaaaaactgg gatacataag
     1441 aaaagaccct actaaaccaa gagctattga agttttagaa cgtagtaaag tgaatgatgt
     1501 ttctggagct aatcaagaaa taatagaact tcctttagta ggtcaaataa cagctggaga
     1561 acccatttta gctcaacaaa acatagagga atacattcca ttccctgcca gtttagtaaa
     1621 gggcagtaac aattttgtat taaaagtaaa aggcgaaagt atgattaatg caggaatttt
     1681 agatgaagat tacgttgtag tagacaaaaa aaatacagcc tttaattctc agatagtagt
     1741 tgcacttata aatggtgaat ctgctacagt taagagattt tttaaagaag gaaatttaat
     1801 aagacttcag ccggagaatg attttatgga accaattatg ctcaatgact cagaagttga
     1861 aattgtaggc attgtaactg gtgtatttag agttattaaa tagctattaa aaaatacata
     1921 aaaaaataag ctattcgtat agataaaatg tgttatctat tgattatagc ttattttttt
     1981 atgtagctca gaagctttat atataaatgg ttgatttttt attattttac ataacatata
     2041 tcacatgggt catttttctt actttctgca attgtgcctg ataatatttt tttacttctt
     2101 gaaagcccac tacaattttt tgtagagtga taactcttcc cttttggagt aaaatacact
     2161 atttccgctg aattagatgt attttgttta tttttaaaag aatcattttt acttgtatta
     2221 ttgtttgatt ttccagagtt tccatcttct ctaacgcctt tattgtaact tccatcttta
     2281 caacttgtaa atactccatc acctgttgat tcaaatatta tatctgaaca ctcatcactt
     2341 ctatgtactt ttactcccat tttttttaat ttattcattg tctcctgatg tggatgacca
     2401 tatgagttac ccttgcctac taaaagaacc gcatattcag ggcttacttt atccaaaaac
     2461 tcttgagatg aactactacg agaaccatga tgagctactt ttaaaaggtc tactttatca
     2521 agtttagata gtatttcttt ttcagtacct tcttcagcat cacctgtaaa taaaacttta
     2581 tcatttccat tagtatatac aaggactaga gattgttcat tagtagtatc tcctccattt
     2641 gtatttagaa cctcaaaata agaatcttct aaatagaatt tattattttc aagtggtaca
     2701 cttggagata aatccttact tgctaaagcg tttataaagt ctctataact ctttgtttct
     2761 gctgaaccat tcgatacaaa aacattttct atattgaaat ttttgacaac agaatcaagt
     2821 ccacctaaat ggtctgcatg agaatgggta gctataagat atttaatatc ttttacacct
     2881 tgattattta gataatctac cattaatttc tcatcatcat tatctccccc atctatcaat
     2941 gtaaaagttt taccatcttg aatcaatatt gcatcactat ttccggtatt aataaaatga
     3001 atttttacat tcttatcact tgatatacta ctagacaaag agtcattatt atttgattga
     3061 ttatttccat tgtctgatgt agcacaacca gttaaataaa ttgatataga tactataaat
     3121 aaaagtatca aagctttaat tttagaattt tttttatttt ttatatttct ttctatcaca
     3181 aactacgcct ccatattcat aatactctag taattataac atgatatgcg tttttatgat
     3241 atttttttac ataatatttt aaataataga ttatggtgca ataacataga aaattgacac
     3301 tgtataattt ttatattata taaaatgaaa tattttatat cagtagtatc cagttatgca
     3361 ttttaaacga ccatttacgt ttaatatatt gcaaactgat tctaaaaatt ataatatgtt
     3421 aagtatcaac aaaaataaat aaactttttc cttaaagaag gcttaaagct cacaccaact
     3481 gccttctttt tttttataaa aatttaatta tttattttga ttaatattta tttcatattt
     3541 tagttctact cttctagatg catctctatt cacgctatta tcaccatttt taatgagttc
     3601 agcttttgat cttcctattg caataatatg cttagtcact ttatctttgt atttatagtc
     3661 acctatttca tctcccacta taaatttagc aacactatat gctcttcttt gagataaatc
     3721 taagttgtat atataacttc ccacatcatc tgtatgacct tctataacta ttttggatat
     3781 atagtctcca taatctttat aaatagtctc catgtacttt ggaacgaaca tttttaacat
     3841 ctctttagct tctggtttta aaacatcact atctatatca aacatggttc tttcaccaaa
     3901 agttacttga ccattagatt tatctacttt cacaggtatt ccactttttt taaaagtttc
     3961 ctcaacagaa gtattgacat tcttagctaa atcttgttca acagaacctg aaatactttc
     4021 tgagctaaat aagataagaa tcacaaccat caatacagta gataataaat ctgtaaatgt
     4081 tggccaaaag ctactctttt caaattctct gttaacagtt cttctatatt tattattcat
     4141 ttaactcatc ctcacctatg acctttattt ctttatcctc tatctcataa atttcttcac
     4201 taaaaggatt aaatacatta ttaacatcat cattaaataa ttcttcacta tcaagaggaa
     4261 cttcatcatt cataaaagag ttattaaaca taacatctaa cttatcttga atatctaaag
     4321 aattttcaaa catcatatcc agtttaactt gtaggtcatt agaaaagttt tcaataatat
     4381 ttgaaagatt ttttattgag tcatctaagg taaacttttc atctaaaagg attttttggt
     4441 tttgagatat tttactttca tgagtcatta atttagttaa taaatcttga gcacttcttt
     4501 catatagggc aaatcctgca tttatatttt ttatataatc tctaattttg aactgacttt
     4561 cagtggcatt gtatatattt tttgaagatt cttttatatc aaataaaatc tctttattta
     4621 tgttatcttg attatcatat ttatcaaaaa gctttacaaa ctttatattt aaaatattaa
     4681 attgcttatc taattttctt acactctggt caaatgtctt aatagaactc tgagaacctt
     4741 ctaaaaggct ttttaaagct tctataccac taataaattc atcattaaag tcatttaagt
     4801 ctttagatat tttatcgaat ttttcaatag cttctataga cttgcttatt tgttttattg
     4861 tattttttac ctcttctact tttaaatcta ttctttctga cttaaaatga gttatttcag
     4921 atgttagtaa attttcagac tttaacatta tttgtatcaa aacatgttca caatctttta
     4981 tcttagtaaa gtaacctata attagagaaa atacaactcc aaatatacta gttgtaaaag
     5041 cagtttgcat actactaata gttgaaggaa gtgaatttat aatgtcttta gtatcaacac
     5101 ataaaagcat tgtagaaaga ccaacaaacg tacctaaaac tccaagtaat atacttattg
     5161 aagatgaatt ttttatactt ctaatctttt ctaacaatgg taaattatta tgctttaaat
     5221 cagatacaac ttcctctata aatgaagtta tatttacgtc tgcataagga ttttctttac
     5281 tatatgtata gtagtcatcc cttatctttt tataaaactc acttgtctta tagtctctat
     5341 tgatttcgtc taattttgaa cttacaaatg aatatactct gttattttct attattttaa
     5401 aaacagaata cacagttatt gcaagtaaaa acaatatagt tattggatta gtaaatattg
     5461 actctattaa attcgttatt atgttactca aatttatccc ctaccttata atttatttat
     5521 ctataattta tacgtacaag ttactaagaa aatacgtaaa taaacaaaat atttatatta
     5581 tatttatgaa aaatggtaca aataggtcat attgttaata acataatata tacgagctaa
     5641 aagttatagg gggtggcata atgaatgaca acatagtaga agttaaaaat ataaatatga
     5701 acttctacac taaggaaggc gagcttgaag tattaaaaga tgtcaatttt aacttgaaag
     5761 aaggagaaat attaacttta cttggtcctt ctggaagtgg taaatctaca atattaaata
     5821 ttcttactaa tcttttaaaa ccaactagtg gtgatataaa gattactgga aatattggat
     5881 atatgtttca aaaagataat ttgcttgaat ggagaaatat aatggataat ataactattg
     5941 gattggaaat tcagggcaaa aaagataaaa aatctttaga tagagttgaa agccttttaa
     6001 agacctatgg attatgggat tttagaagta tgtatccaaa agaattatct ggaggtatga
     6061 gacaaagagt tgcgcttatt agaacacttt ctgtaaatcc agatattcta cttttagatg
     6121 aacctttttc agctcttgat tatcagacta gactactagt ttgtgatgat gtttatagta
     6181 ttattaagaa tgagaataag tctactattt tggttacaca tgatattggt gatgctatat
     6241 ctataaaagt gcaactttag attatcaaca ataatcacct ttaaaatttt ctaaactcat
     6301 cataaagttt tcaaagacta tgctttgtaa taaattaata aactcattat ataatattac
     6361 aaatttatat aatgagtttg atttctcttt aagatacaaa aaaatatcat ttatacttgc
     6421 ttgttacttc tttactatct cttccaaaat attttcttat aaaataagaa ggtgtacaac
     6481 tccaggcatg acaatagctg tttattatcc ttgaaccata aggagataca tatttatcat
     6541 ttggattata tagctcccag aaacaatcag ctccatcctt aaccatttca ccccaataac
     6601 ctcttataca ttcaagagct ttttcttttc tattatttaa tattaaacat tcaactaaat
     6661 gatgatatgc atatggagta actatatcca tatcaggtgg attatctaat aagttatcta
     6721 atatctttgt atttgtttct ttatcaaata catcagcaag aacacaccat atttgagata
     6781 tccaagaaac ttgcttttca ctaccactta caaagaatcc ttgtttttca tcccatagat
     6841 gatttttagc accttgaacc actctatcta tttcatactc aagctcttct tttttttcat
     6901 tatcgcctaa tattatagct aatttatgag cttgttttaa agcatatatt aatactccct
     6961 gtgcacctgc ttgtttattt aacccttctt tccaatctaa gaaacaccac caatcatcac
     7021 tatcataaac aacaccatta attccaactc tttctaaagc taacttagct tgaatgtatg
     7081 caactttcca taattcattt aaaatttcca tatccttagt tgcttcatag tagtcatgta
     7141 aacatgatat gaagaataag gaatagtcaa ataaagcagt atcatccact aataatttag
     7201 gttctataaa taaacatgct cctacttggc ctctattttg agttaatcct gcgaaaagat
     7261 ataaacatct ttttactaaa tcattatttt taaaagtttc ataattcgtt atagcttgta
     7321 gtcttaaatc tcctatccaa aggcgtctat ctcgttttgg cccatcttca aaaacagtct
     7381 gcatgcaatc ttgcatagtt ttcaaagcta ttttatcgat tttaattaag tcatcatcaa
     7441 tatttgatag ataatttatc tctgacatat ctactgacga tacaaaatta caagatacat
     7501 cttctactat tactttatat ttaggagatg tatctaatac ctttatttcc aaatatctaa
     7561 atgcatatct tcttttcata ttaattttat caggtaaaat atctatatgc atagtctctt
     7621 cttgaatcca actactactt atgcttcctt tgtaagttga actatcctct cctatttcac
     7681 ataatgtttc tcccaattta attcttaaat gagtaggtgc atctggtggg ctacctattg
     7741 gttttatttt aaaatttaca tatcccactt ggtgatttcc aaagtcaata caaatgctct
     7801 ctcctttacc taaacttata ttagatattt catctattga ttttgtatct ataacctccc
     7861 aatcattttt acccatttta atattaacaa tttgcttagg tttaattagt ttttctataa
     7921 ggattggctt tagtttttct gctttatcta tgaaatcttg attaatttcc attctgaagt
     7981 cttttatact atttgtaata ttagacatgt ttattctcct tgattgtgtc attattattt
     8041 attcctcact tatttttcta gcatctaaat ctgctttgat tttaggatat tgcttatcta
     8101 agtcataaaa tttcatagtt atcattgagc atattaaaag tgccattgga atataaacat
     8161 acattatttt aatagctact aatgcacttg ctggttgaac ttccgcttta gctatgtatc
     8221 caccccaagc taggagtgct ccacctaaac cgcctgatat agcttgtgca cattttccta
     8281 aaaatccatt tactgagttt aatgttccag ttgtatttat cccagttttc caaatcccat
     8341 aatcaacagg gtcagcctgc attgagaagt atatactttc ttttattcca tatccaaagg
     8401 cagaaactat agaacctgct ataattaaac ttatatttaa attagctatt agtattattc
     8461 ctagacctat aaattgtaca gctgctgaag ttatgtatag attacgtttt cctaatcttt
     8521 tagctagaat tgggactaag aagtttgcaa gtattggtat tattgacata attgtagcca
     8581 ctgttgttgc caacgactta cttccaacat tgtatgaaaa gtaatatatt aatgcactag
     8641 attgtataaa gaatccagtc cacatgaata atatatttaa ggcaaatagt ttccaaggtt
     8701 tattttcttt taatgatttt atagtttgaa ataatgttac attttcagat tttatttcaa
     8761 ctcgttctct tacatttgag aaagtaaata agaatattgc acatcctata aatgcaaata
     8821 gcatcataac tactttaaac cctagagctt catcagtgcc tccaaacttt cccactaaag
     8881 ttaaagcact ataacttact attgttgaac ctaaaaatcc aaagaatttt cttgatgttg
     8941 ataatgctgt tctctcatta gtatcatctg ttaaacttgg taatattgaa gccattggaa
     9001 tatttactat agtataagtt aaagataatc caatatatgt aacataagca taaattaact
     9061 ttccacttaa tgatatatct ggaacgctaa atgctagtac tgcgaataat gcaaatggta
     9121 ctgcaccaaa taggaaatac ggtcttgatt taccccaacg agtatttgtt ttatctatac
     9181 aataaccaac caataaatct gtaattccat caattaccct tgttacaaca aacataaaac
     9241 ttattgctac agcacttaat cccataatat ctgtgtaaaa atataaaaga tataacgata
     9301 tcatttgcca aattaggtta caagctaggt cagatgaccc atagcctatt ctttgacgcc
     9361 aaattcctgg tacattcttt tcatcaacaa cttttaaagt agtcatttat aatcccccct
     9421 tattttgttg tttttattat ttctaatgac ttaacatcat tatatccttt agaaaatgct
     9481 tgaactattg tcgtttttaa ccaatttaat tgatttattt tcatatttat gatattttta
     9541 ttttatattt aaacaatctt agagatgaag gagattttat gagtttaaat aaattatttc
     9601 aaaatcttta tatttataat gaatctgagt tattttataa aaattattat tttgcaaaac
     9661 aaaatccaga atccctaaaa ctatttattg ataacttaga tgaggattac atagttaata
     9721 ataatctaat tattccatat atttataatg aaaagattcc tcaacttatg aaagatgaaa
     9781 cttattttga gtgtaacagc aaggataacg ttatacttat aaaacataat cgctacacac
     9841 ctcaatttgt gcatgagcat atattctttg aaatgattta tgtcttatca ggtaagtgct
     9901 atcaggtaat taatcatcat aatgtacata tgaaagaagg agatatttgt atagtttcac
     9961 ctggagtaaa acattctata ggagtatttg atgatagtat tattatcaat gtattaatac
    10021 gtagaagtac ttttgaggat gctttctttg attttttaag aaacaacaac ttgctttcat
    10081 ccttcttttt aaatagtatt tataaagaca aacagaatga ttacatactt tttcatacaa
    10141 ataatgataa atcaatagaa atctctattt tagagatgct ttcagaatat aatgaacaag
    10201 atagatatta taataatata cttaatcatt taataatggt gttttttgca aaattactta
    10261 gagactatga agatagtata gaagttccta atgtgacgaa aaaaggtact ttaaatcaca
    10321 caaatattct tacttatatc caagataatt atgttgatat aaccttagaa gaacttgcta
    10381 ataaatttca cttttctata ccttattgtt ctaaaattat taaagaaatt gcaggttgta
    10441 attttactca attattacaa agaattagac ttgaaagagc tgaaagtttg ttaatgaata
    10501 caaatattcc tattgctgat attagcaaca atgttggtta tccaaatgta gagcatttta
    10561 ttagattatt taacaaaaaa tataatatgt ctcccagtaa atatagaaaa aatactacta
    10621 aagtataaca ttgttcataa ttaaaggcta tgaattaaaa ttcatagcct ttttaaatat
    10681 gtgtgtttaa taagagtttt tattttaaac tatttatttg attattatta acttaatttg
    10741 gttcttgtta attttgagat gcccaatctg ctggatatgt tacatatata aatctagcat
    10801 aatcaggtac agaaaattgt atcttacttc cttttggtat aaatattaac tcacctgaag
    10861 aagcagaaac ctttcttcca tctattatta tatctaaagt tccatctatt acataatcta
    10921 tttcatcata gtttagagtc caatcaaaag ttgtttcttt catttccatc ataccacaac
    10981 ctaatcttgg gctttcctcc aatgtaaata aatcctttgt gtaaactaca tcacttggat
    11041 ttcctgtatc taatctatca gattcactaa cttttacagt tggtaacttt atagaagtaa
    11101 ttccactaat atctttattt ctaacaaaat ctactgtgtc cttagttcca cttatttttt
    11161 cttctattat ttgtcttact aatgtttcaa ttaaattttt atctatattt gatatatcca
    11221 tcttatacat tctccttttt tagtgtctta tttgcgataa ccatagccaa taatactgct
    11281 gttataccgc caactaattt agcaactatc attggtgtta tcatattaga attaaatcct
    11341 gctgtaaatc ctaagtggtc tccaaatacg aatgccgctg atacagagaa tgctacattt
    11401 attatctttc ctctattgtc catatctttc atcataccaa acattggtat acagttagct
    11461 aaacttgcaa caagccctgc tgctgacaca tcattcattc caagaacttt tcctaaagcc
    11521 attaatggtt tattaaatac ctttgttata acaaacacta gtgggaatgc tcctgccaaa
    11581 actattgcta tatctccaac tatttctata ccttcactta ttggcttcat tccaggtatt
    11641 acaactatcc ctgttaaagc ttcaactata gcagcagcaa atcctaaagt tataactatt
    11701 actacaccct ttccaaagta tgtgaaccct tttatcatag cattttcaaa tttcaataat
    11761 ccaagtgcta taactactgc aaatattatt attggtacta agtttctaag aaccatcatt
    11821 atctcaaatc ctgcaactaa tccaccaacg aatgctccaa taggtattgt tattatacct
    11881 gcaagtattc ctgttgctaa gaatttttgg tcttcttttt caattattcc aagtgcaact
    11941 ggtattataa atactattgt aggtcccatc atagcaccta ctattaatcc accaaataac
    12001 ccagcattag ggtcttttgc aagttcaagt gcaagtggag caccacccat atcattagct
    12061 aataaacttc cagcaaacat agctgggtca gctccaagta tattaaatac tggcactacg
    12121 attggtctta atacatcagc aagaactggt gctaaacata taacaccaac cattgcaact
    12181 gcaagtgaac ccattgccat tataccttct tcaaattgct ctccaagacc aaatttgtta
    12241 cctatacatt tatctatagc tccaagcacc atgaatatta ccattacata gattataatt
    12301 tcgtttatac tcataattcc ccccattatt ttattagact ttttattatt tgactgtcta
    12361 ctgatggtat tacagtctta tatattactt ctttttctct tactacatct tcactttctt
    12421 ttaatgcata ttctaaacta cttaaatctc ctgctactat atatactaat tttccaccta
    12481 tagcaaaaga taattgaagt tttactaata ctaattcgcc tgattttaaa gtcctatcaa
    12541 gcatttttat tccagcacat accttaccac tttcaacaac acctatagag actatattat
    12601 ctaacttttg atacttattt ttaaatccat ctaatatttc ttgtgaaatt gcatttatta
    12661 caaagttatc tacaatatat ccttctccaa gttttattcc ataatctaca cattcattta
    12721 cataagaagt ttctccacct actactatta gaaatttacc tggacaaata ctttttaagt
    12781 acaatacatc aactgaagat ttctttatca tttcattgga tacttctata cctttagcaa
    12841 tacttttaaa ttctatagct cctatacttt tattcatcaa acacctcaaa cgaatcgact
    12901 actccaacta tagttgcatc tactggaaca tgagcttttt caatcgactc tctagctgag
    12961 gaacctctgg atataagaac caagtctcca tttcctgctc ctgcattatc ggctgcaacc
    13021 atgaagcctt ccttgtatgt gcctttagtt tttaaaattt ttacaactaa aagtttttgg
    13081 ccacataatt tttcatcttt tcttgtggcc caaacattac ctacaacttt tcctatatcc
    13141 ataaaattac ctccttatac tttctttagt ttgagatggt gtattctcat aaaatctaca
    13201 gctaatggtg ttattatact ttttttatta actagtatgt ttttaactcc atttatagtt
    13261 ggttttctta agtcagcttc cgatataagt tttctatttc ttaaatctaa actaaattca
    13321 ttatctattt ttacactttc tacactggtt aaatttaaaa catctttatc taaagctttt
    13381 gtatcttctt ttttagaacc tatagtactt tctagttctc tttttatgtt taacttattt
    13441 ttagttatag catttaagtc ttttataatt tctacaccaa atttacatat ttcatcttca
    13501 aatgatataa atttcttata taatgctttt ggtgcagtat ccttatattt tttgtattca
    13561 attccttcat ctaaaacata cacttttttg ccactcataa tcatttttag tataaatctt
    13621 tcttcatcac ttgttgaatt tccaagtgct atattcgata atcccctcat acaaagtctt
    13681 gaaactatca ctatttcaca ttcctttata cttttatcaa attccactat ttcaaattca
    13741 tcttttaata agttaaattt attggtgtct tgttcaaata caatcacagc tttaggttta
    13801 ttagatattt ttatttcatt gctatttact tttttataga tttcttccat tattaaatct
    13861 acaagattat cataattcat attaaatcct ccccagaggt aatttatata gtaggttaaa
    13921 tttatacttt ctttattatt ttagcaacag tcccttttga ataaccacat gcattggctt
    13981 catcataatc tatatgcata taagttttaa aactgtcact cactcttatt actacatcat
    14041 caaatgttaa aggtctttga cccatcactt tggcttcaac aatttcttta tcacatacat
    14101 tgaaattttc agcgtcttta ggagtcatgt gtatatgtct cttagcaacc attaatccct
    14161 tatcaagttc aacttcacca tgttgtgttg atattttaat acttaaagtc ccttctaagt
    14221 ctccactctg ttttatagga gcttttagtc caagagttaa accatctgtt aaagatattt
    14281 ctacctgagt ttcatttcta catggtccaa gtacaactac attttttata cttcctttag
    14341 gaccagttat tgtgactctt tccttacaag catattgacc gggttgagat aggtctttta
    14401 gttttgtaag agtatatcct ttaccaaata atttatctac atcttctcta cttaagtgaa
    14461 cgtgtcttcc tgatgcttca acttctataa atgcttcttt tttaactcta gttattactt
    14521 cttccactat ttcatttaat agattctcca ttgtgtcctc caactactta tacttattag
    14581 ttctaacttt atatatcatt atccaaaata ctgacgataa tctattaagt gcctttatta
    14641 aatcttctct atgaacattt ccatactcac ccttaaatgc ttcataggct gcaagttcaa
    14701 cctctcgtgt attacttctt atactattta tagctactac tacttctccc atttcataac
    14761 ttgggaactc atgtcctata ccaaagtatt ttttaggata gtgagattgt tctctaagtt
    14821 cttgaggagt taatcctaaa agtttaaagt cctctagttt ttcttctaaa acttcacatc
    14881 tcattatatt tcttacaaag tctaaaactt cttgcaattc cttaacgagc tttggcatat
    14941 ctagtttctg acaaagtatc tgtgccttta gtatttctga ttcaagagaa tctatttttc
    15001 ctctcaatat tattctttta tggtctttaa aaactaaaag atttccattt aattgagtca
    15061 tgtgttctgg tttttcatcc aatttagcac caaatacagt agtatattta tatgtttttt
    15121 ctttaactac ttcctttaca acttctttag gctcattatt tacatctgaa agtttatcaa
    15181 catatttaag ttcaatactt ctctcagcta aataagattt tgcagaagga gttattattt
    15241 gtcctttttc tatataaaat tcagttatat tatttttgtt ttttccaatt tttctcttta
    15301 tttcgttttc tgttaaaaca cccattttat cacatctcct attagatgta taaaaaagac
    15361 tacctcaaaa tacattgagt ttattagtaa gactcatttt tgagatagcc tctttttcaa
    15421 tcaaaattta aattttaaaa ttatttttat attactcagc tgatactttt ggtaatatag
    15481 catctacttc aaagtgtggt cttggtataa catgaactga tattagttct ccaactctct
    15541 cagcagctgc agctcctgca tctgtagcag ctttaacagc cccaacatct cctcttacca
    15601 ttacagtaac taatcctcca cctacttgct cttttcctac taattgtaca tttgctgctt
    15661 taaccattgc atctgctgct tctattgctc ctactaatcc tttagtttct atcattccta
    15721 atgcgtttgc acttgccata atcttatcct ccaaaaattt aattttatag tttatatttt
    15781 tagaaattta tagctatagc tataagtttc attgtttatt ttagttatcc tagttctttg
    15841 actagttttt gaacaagaag atttattaat tcttctttat caattgagtc aaacattgat
    15901 ttttcaactt gagatttatc tcttccaagt tcttctattt ctttgattcc ataagctact
    15961 cttttaacat ttattaaatc aagtggtcct atattgtttg aagttgaact tccacctatt
    16021 gcaccacaac caagtgttag tgctggtaca agatttgttg atgcacctat tcctccaagt
    16081 gctgaaggag tattcacaag tattcttgat actggcatgt taagagcaaa tctttttacc
    16141 aattcttcat catttgcatg catggagaat gtgtgaccgg caccttcatt taataatatt
    16201 tctctacatc tgttcataat agcttctaca ttttcttcaa catagaacgc tagtattggt
    16261 gtaagttttt ctcttgaata ggctacattg tggccaacct tattttctct tgcaactaga
    16321 acttttgtat ttttaggcac atttgttaag tctgctaaat ttgctattac ctcaacactc
    16381 ttaccaacta tttgtggatt catagtaccg ttagctctca atataaattt tgctaatttt
    16441 ttactttctt cttcatttaa aacatatgca ccttgatttt taagttcttt taaaacagtc
    16501 tcttccatag atttttctat tactatagat tgttctgatg cacatataac accattatca
    16561 aaagttttag aatctattat tcttttgatt gctaacttaa catttgctga tttatctata
    16621 aatgcaggtc catttccagg tccaactcct atagctggtg ttcctgaaga gtaagcagct
    16681 ctaaccattg cttctcctcc agttgctagt attaagtttg tatctttatg tttcatcagt
    16741 tcattagttc caactatact tggattagtt atacataata ttgaatcttc tggagctcca
    16801 gcttttatag ctgcatcact tattatatta actgtttcaa gtatgcaatt ttttgcattt
    16861 ggatgtggag agaaaactat agtatttcct gcttttagag atattatagt tttgtatatt
    16921 actgttgatg ttgggtttgt tgaaggtata agacctgcca cgactccaac tggtgtagct
    16981 atatctattg tatttgcttc tttatcttca ctaattattc caatagtttt tgtgttcttt
    17041 aaagtttcat aaactcccat agatgcaaaa gtatttttaa ttactttatc ttcccatttt
    17101 ccaaatccag tttcttcatt tgccatttta gctaatttag tagcattatt aaatgctgca
    17161 tctgcaatag cttttactat tttatctatt tgctcttgac tcattttaga aagtttatct
    17221 tgagccactt tagatcttct tacaagagtt ctgacttctt gtatagatac taaatcttta
    17281 tctaagagtg tcataactat ctatcccttc tgctaagctt tttaactaag tttattaatt
    17341 cttctttttt aagaacattt aaatctttat aagttattga agaatctaat ttcttagcca
    17401 tagctttaag ttttttcacg ctgttctttg ataggtcatc tctatcaaaa tcgaaatttt
    17461 ctattttaac ctgttccaat acttttttat caatgttagt attttctatt tttcccttat
    17521 tagcttctgt taaaatatca tcaactgttt caatttcttt gttgatttta tctatatcac
    17581 tatataattt ttcaatattt ccactattct cagtgacatc taaaattaca tcttcttttt
    17641 ctgataaatt tttcgataca ttatcagttg tttccaatgt aacctcaaag tttttctctt
    17701 tatcttctga aacttcacag actttaggtt ctttagtttt gaataaagca tctgttactt
    17761 catcatggat tcttggtata atatgtgatg ttcttaatgt accaactctg gaagctgcta
    17821 tttcacctgc ttctaaagca gattttacag caccaacatc cccacatatt gtcaaagtaa
    17881 caatacctgc cccaacctta tcaattctta caatattaac atttgcagat tttaaacaag
    17941 catcagaagc ttctattgca gcaacatatc ctattacttc tattaagcct atagcatcca
    18001 tctaactatc cttaaaatcc tgttggattg tctgcaactg attttactgc ttctgcaaat
    18061 gcatcacatg ctgctttaca agccgattga gaacctgtaa gtaatgctcc accaaagttt
    18121 gtttcagttg gaggtgcaaa taattcacac attttaacat ctgctgcttt cattgctgca
    18181 tctagtgcat acattgcttc aagtggtggt gctactaagt aagctaatgc ttcaccttct
    18241 cttattcctg ctacctctga aaggtatgta cctgttcttg atacgcagtg agcataatat
    18301 gctattgaat catcatcatt tgcacttata aaagttgctc catattctag aaaatcaact
    18361 actgcattta gaccacttct tacttctgct gggcttggtc ctgctattat tcctaatact
    18421 tcaccagcaa gttttgttga agcatttgca gcacctgcat acattgattt tgcatataca
    18481 acatcaactt ctgaagcttt agttgcttca tcaagagctg tgtacgtaac atcatcacaa
    18541 tctgcagtta ttaaacctaa acttttgtga tgtgatttta gttctagttt ttgtgccatt
    18601 tctggactta tattcgaaat tattttaatt cctagtatat taggacgaat tacatcgttt
    18661 ttcatattct acactccctc gactttattt aaggtctatt cctgaagctt ttttatctaa
    18721 catagttttt ataagttcag ctatatgagc acctgcttca actgcagttg ttccaccttt
    18781 gtgaatgttt gatataactg ttctttttgc ctctgccatt cctatatatg gcttataagc
    18841 tatatatgct gacattgatt cagcagtaac tagtcctggt ctttctccaa ccaacatgca
    18901 tataacttca cagcctaatt cttcacctaa gtggtccata gcaccaaccc tacaatgttt
    18961 aacaaataaa atttcacttg aatctatacc atacattttt agaccttgtt ttatagctgg
    19021 cacacagtct tttaaatttg cctctatcgc agctgaacta agtccatctc caactaatat
    19081 taaaaccttt tgatttgtac caaatttaga ttttatatta tttatagttt ctggagaaaa
    19141 ttttcttccc aaatctggtc ttgttaagta ttcatcttta tctttacata aagtttctac
    19201 tgcaaacata ttattttctt ttataaattc ttcatctaca taagagaata cagcatcttg
    19261 tgcagcagca tggtctgctc taaatcttaa aactgtttct gttttatatc tagtgccggt
    19321 tcttcctata cctaatcttg caggtgtttt tgctttcata tctaaataag cttctgcatc
    19381 cttaggattt tttactagta attgctttct tatatcaact tctgttatat catctataca
    19441 agcattgttg tctactacaa cttcagttgc ttttttaaca gtttcagata caacattagt
    19501 atctaattct ccaaccattt gtccaactaa ttgttctact aatgctttta aatccttctc
    19561 attcatatta aatacctcct atcctaataa tacagacgcg tctcccgcca actctgttaa
    19621 cttaccatct ttagagaatc ccatcttttc catccaagca tcaaattctt ttattgctgt
    19681 tagcccaaac atttctctaa gtgatgctgt ttcatggtat cctgttgttt gatagttaag
    19741 cataacatca tctccatgag gtattcccat aaaatacgta catcctgctg ttgttaatag
    19801 tatagctaag ttttccatat cattttggtc agccttcata tggttagtgt aacaaacatc
    19861 aacacccatt ggtaagccat gtagtttacc cataaagtgg tcttcaagtc ctgctcttac
    19921 aacttctcta gcatcatata agtactcagg tcctataaac ccaacaactg tgttcactaa
    19981 gaatggttcg tattttttag caaatccata acatctagct tccatagtaa cttggtctat
    20041 tccatgatgt gcttctgaag ataattctga accttgtcca gtttcaaaat acataacatt
    20101 aggtcctgta gctgtaccat acttcaaagc taactgtcta gcttcttcta tagtatcgcc
    20161 attaaatcca aatgcttcat ttcctttttc agaacctgct atagattgga atatcaaatc
    20221 tgcaggagca ccatttctta ttgcttccat ttgtgtagta acgtgtgcca atacacatgt
    20281 ttgagttggt atttgatatt tttgttttac ttcttcaaat ctttttaata ctttagtaac
    20341 actttcaaca gagtcatcaa ctggatttaa tccaagtact gcatctccta taccaaaagt
    20401 taaaccttca agaagtgatg caagtattcc atctgggtca tctgttggat gattaggttg
    20461 aagtcttgct gataatgttc cttgttctcc tatagttgta ttacagtggg cagtaaccct
    20521 tatctttttg gctgcataaa ctaagtccat atttgacatt aattttgcaa ctgctgctat
    20581 catttcagaa gttaatcctc ttgatattct tcttatatca agacttgtag tatttgaatc
    20641 taatatccat tctctaagtt cggatactgt ccaatttttt atttcttcat aaactttttc
    20701 atttacagca tcttgtatta ttcttgttac ttcatcttct tcatatgcta ctgcaggatt
    20761 atttcttata tcctccaatt ttatatgtga taaaacaact tttgctgcta ttctttcttc
    20821 agctgattct gctgcaagcc ctgctaattt atctcctgat ttttcttcat tagctttagc
    20881 cattacatca tataagcctt taaactgata agtacgtcca aataattttg tctttaaaat
    20941 catttactta cctccttcta ttaattaaaa actaatgttt ttattaccac aggtaaaact
    21001 ttaccttcaa ctataggttt tccaatgtcg atatagtctc cattttcaac cttgacactg
    21061 tctatacaaa caatctcttt tttataattt aataatgcat atatagcttg acctaatact
    21121 tttgccatat cattttcaac aattactata aaaggtaaat cattttctat aatttgttgc
    21181 attccatcta caaatccttt ggcatactct tgtatatcta aaaaggttgg atttttcttt
    21241 cctatcatgg ctatagcaac tttttgtaaa tcattttcta gtttaaacca attaagttta
    21301 ttttttatag cttcactgat ttgttttgaa gtacctgttt cttcatcaat tgataatttt
    21361 aaaactggca agtttttaag tggaaacttt tcgctagtgt aagttattgt acttccactt
    21421 acttcagtag tatgagaacc agctccaacc actgtagctc ttattgtttc catagattta
    21481 actacattta cttttttaag gtatttacaa ttttttattg cttgtccaag taaaattccc
    21541 aagtcaccat attcaaaata atcttttatt tccccatcat agtaaatata atcagctact
    21601 ccaccagaga atgaaacatt tttaatattt tcatctacac ttattgattt attggttatt
    21661 ataaaatcaa ataaaggtgt ctgttttttt aatcctatgc tttccattaa taggtcaacc
    21721 attatatcta ctattggttt tgtattttct atggtagcta ttgtacctac ttgaagattt
    21781 aaattattat cttttatgat tttatctatt ttaggtgata tataagttat ttttttactt
    21841 attttatcta tctttataag ccttccacct atatccaaac acccagtatc aattacttca
    21901 ccccttttaa atgaagctat attactagtt cccccaccta tgtctatatt tacaattgta
    21961 gttgaatgtt cttttgaata aatatgagct ccagcccctt ttcctgatat tatactttct
    22021 aaatcaggtc ctgctgttgc aacaacaaag tccccagcaa atccactcaa agtatgaagc
    22081 acatcatttg cattttcttt tctagctgtt tcaccagtta ttattactgc tcctgtatgt
    22141 atatccactt tagatatatt tgctttttta tattcatttt caactatgtc ttttatttgt
    22201 tgaccattta tttcattatt tgaaataaga ggagtgaaat aaactttact tttataaata
    22261 atttctttgt ctactatact tattctaggt actgaaaaat ttgattcagt attttctact
    22321 attaatttag aaaaaattaa ctgagtagta cttgttccaa tgtcaatacc aacacttaat
    22381 aactcttctc tcattttctc acctcctaaa atttaagtat gtcttaaaca agcaaagagg
    22441 cttaaagtta taataaatat ctattaaatt gaatatatac ttataatttt tataaataca
    22501 ttcaattaac ttaatctatt aataacctta agcctctttg ctcaattaaa actactactt
    22561 tgtagatgag ttattattta aatattaatt tgtgtaggta tagtaattaa tatttctgta
    22621 ccattcttat tactttttct ctctatattt ccttttaaca tttgtgttat ataactatct
    22681 attatatatg tccctaaact atttttattt atattatcgc tatcaaatcc aactccatca
    22741 tctataatag caatatttct atagttatta tctttattta ttaatatttg tatagtacca
    22801 tgttctctat ctaaaaaggc atggtcataa caattttgta gtatttcatt tataactaaa
    22861 agtattgctg ttgctttttc tccactaatt ttaaaatctt caccagttat acgtatatct
    22921 atatctttac taaataaata gctaccttga atatttgaca ctaataaatt gataccttct
    22981 cgtatagata tattattatc aacttcttta gatagtaaat aatgagtcga aagtattgca
    23041 aataccctat ttattatatt atccaagcac tgcttaactt catcattttg agataatcta
    23101 ctttgttttt ttattataga tattactgta tgaagattat tttttactct atgatgagct
    23161 tctcttatag ctactgtttt taaaactaat tcttcttctt ttcgctttaa atcacttata
    23221 tcttgtacta ttttgcaaac cttaaattca tcatctttga tgtatatggt ttttatttta
    23281 aaaaacatat tatctattga aacttcattt gtctcatcat aattttcatc cattaatata
    23341 tcttcaaatg tattggtttc tatatctaag tcattataat gcatatcttg tatatcatat
    23401 ccaaacccaa gatttttata tatctcagtt gcaatacgat ttttaatttt taaatttcca
    23461 tttttatcaa aaattagtat agctgaattt aaattatcaa ctaaaaagtc attatttcct
    23521 ataagtttta taaactgatg taaattttta ttttcttcgt cttcactagc gttaaactca
    23581 ttcttgactt cactacttat atctttttca actattaata ctgctaaaac tttgtcatct
    23641 agcgttattg gctgaatatt ttgcttgacc agtttatatt cttgtgtcaa agcttttata
    23701 tctcttactc tttctcctgt ttttaagcac tttataactc ctggttcatt ttcaattaaa
    23761 gcctttttgc ctataacatt ttcacagtac attgactttt ctttaggtct accatgtgcc
    23821 acaacaattg cttcatcatc aattttactt agtatatcta taaaaacatc agcctcataa
    23881 aaagaagcca ttaaatctaa ggattttgat acttctataa ttttttcaat ttcattatta
    23941 tttaagtatg tttgtgcagt tgatattttt tttatcgtat tagtcatcgt atcctattat
    24001 aatcattttg gcaatttcca tcattgtggt tcttttatcc atacttaatt ttcttattct
    24061 agtgtatgct tcatcctcag aaatcttaaa ctctcttata agtatacctt ttgctctttc
    24121 tactaatttt ctttcattca atttagaatt tactttttct aaatcttttt tcattttttc
    24181 aaattcattt accttattta aacacatttc tatagttgga ataagtgatt tttcacttgc
    24241 tgtctttacc aagtatccat aagcacctat acttttagcc ttttctacgt attctttatc
    24301 ttcaaatgaa gttaataaaa gtactccacc agctagattt tctttacata taatttttcc
    24361 tgcttttata ccatcaagta ttggcatctc tatatccatt ataactaaat caggtttgtg
    24421 ttttttacac atttcaacag cttcaaatcc atcacttgct tcgcctatta tttcatagcc
    24481 atgactttct aatatatctt ttgtatccat ccttaacagt ggttcatcat caaccaatag
    24541 tatttttttc cccatgttcc tacctcgctt aaagattaaa tcttcttaaa atagtttata
    24601 cattttttta ttttatcata ctttaataat atttttgaat aaaaaaatat tttcccctta
    24661 aacttcatct atatttttag atttttctag atatttaaat aatttttcaa gtccaatatt
    24721 atcaactgtg tctattttaa agatttgact tgctcctgct agtgatagtc tttcataagc
    24781 tatattgact tcattttcag tagctttatt tattttagtt ataattccta ttacgtcttt
    24841 gcaaaacata cttgcaaaac ctggtgctat ataattttct tcttgtgtgg catcatacac
    24901 tattgctata attttagcat ctgctgatgt tgttattaag gcattgtaca ggtttctatt
    24961 ttccatatac tcaccaggag tgtctattgc attattataa agttccactg attgagtttt
    25021 tttatagttt aattctaact ggtctaattt ttgacataaa gtagttttac cactccctgt
    25081 ctttcccata aatattatat tcttcattgt attattatgt ccttgttatt tttgtagatg
    25141 agaagtttaa catatttcct aagacttcca aaacatcatt taacgctgat tctacagaac
    25201 ttacatcacc tgatattact actgaaccac taaatctatc tataaaacct aaagatacat
    25261 ttgaagcctt tgtagcaaca tctgctgcta ttattgacgc ttcacttggc gttattgtaa
    25321 gtattcctat tgcatctttt ttatcaagta ctaatcctaa cttcttatat atatcttcat
    25381 tagggttagc tatgatatgt gctaatgtaa cctgttttcc aggaacatat tcttgaataa
    25441 ctctttgctt agattcttca gtcaaattta ttccctactt tctttaaata tagtttacta
    25501 aaaagataat tgcttaaaat aaaaggagct ccataagaat aatattaata ttcctaagaa
    25561 gctccattgc tttctaataa agtatcacgc ctttgtgata ctttcattat agttttatgt
    25621 tttatatttg tcaatatata atttatattt ttaaaaacat tttccttatt tgtataagat
    25681 tatgtttaca tgtaataaaa tgtagttatt taatattatt taatatgttt attatatcat
    25741 cttcagttgg taccttagga ttagttaatg tacaagtatc ttttatagca agctttgcta
    25801 tttctctttc ttcattagtt atatcttcta aacttaattt gcactgtgtc aatttagttg
    25861 gcatattcat ttctttttgt agtttcttta tttcatttac caaatttcta acagatattc
    25921 ttatattagc attcccaagt cctactaatt ttgctatttg tgcatatttt ttagctgtat
    25981 cagagtaagt tgtgttagta tatcctacta ttgatgaatt atattctatt acatgaggaa
    26041 gtaatataga gtttgttctt ccatgtggta tatggaattt tccacctaat acatgtgcaa
    26101 tagaatgatt tattccaagt gatgcttggt taaatgccat acctgctaaa caagatgcat
    26161 tgtgcatttt ttctcgagct tctatatcat ttccatcttt atatgctttt attaagtatt
    26221 taaatactaa ttttatggct ttttcagcta gtgcatctga aaaatcatta gaatttgtag
    26281 atacataagc ctctatagca tgtgtcaata catccattcc tgtatctgct gttataaagt
    26341 tcggtacagt ttttacaagc tctgggtcta gtattgccat gtctggaagt aaatcatcac
    26401 ttactaatgg atacttacat tttttttgac tatctgttat tacagcaaat gatgttactt
    26461 ctgaacctgt tccacttgtt gttggtattg ctataaactc tacttcgtta atttttaata
    26521 tttttttaga aaagtccatt atagattttg ctgcatctat tgcagagcca ccacctaatg
    26581 ctattattac atctggattt atagatttaa atgcttctat ccctgatact actacttcta
    26641 ttggtgggtc tggtactatt tcttcaaaaa cttgatatgt tgagtttacc aaattagatg
    26701 ttattttatc tattgttttt gatttcacca taaatgggtc tgttattatc aatacatttt
    26761 tgttttttag ttcttgtaaa gctttcagtg aaccttctcc aaattttatt ctagttttaa
    26821 tctcaaagtt attcaaattt ctgtcctcct taagttaaat acgcaaaaaa gctccataga
    26881 ctttatctat ggagctccat tgctctttta acacttcatt gtgctatatt taaaaatata
    26941 ctatttgatt aatataatgt caagtcatag tgtacaaatt caataatata taaaatcaat
    27001 aatcaaagct tatgtattag ctttacttat atttaattct ttttctaaag tactaaatgc
    27061 ttcatttaaa gcttctttag gatatttttt acttattata tttacaaacc aactagcttt
    27121 attcatttct atttcgttaa gtttagcttt atctttaggg aaaatttcta taacattgat
    27181 ttccatatac aatcccccta taaatttttg ttatagctat tagttccaaa aaattatctt
    27241 ttaaaatatt taaattttaa ttttttcata ctaacttatg attttatatt aataatctat
    27301 gaaattagag atttgtcctg atacaattaa ataccaaaat ttattattga tttagaactt
    27361 gaatagtaaa gaaataaaat aaatagtcat atattataaa aagtagaatt atataaattt
    27421 acctaattaa taaattcatt ctaataagtt tacttaaaag attgtatata aataattcta
    27481 aatatatgtc ctaacttata ggaggtttta attttgaaag ctgcaattta ttcaagaaaa
    27541 tcaagattta ctggaaaagg cgactctata gaaaatcaaa tacaattatg tattgattat
    27601 gcaaagtcta ttggtataaa agattttttg atatatgaag atgaggggtt tagtggtagt
    27661 aatatagata gaccacaatt taagaaaatg atgcaggatg ccaaggataa gaaatttaat
    27721 tacctagttt gctatagatt agatagaata tcgagaaatg tatctgactt ctctacctta
    27781 atagaaaaat taaatgaact aaacatatca tttatatcta tcaaagaaca atttgatacc
    27841 tcaactccaa tgggaagagc tatgatgttt atatcaagtg tatttgctca actagaaaga
    27901 gaaacaatag cagaacgtat aaaagataat atgtatgaat tagctcgtag tggtagatgg
    27961 cttggaggca atacacctca tggatttaac agcgaaaaaa tcacattctt agatgaaaac
    28021 ctaaaagaaa gaagtatgta taagcttcaa gtaaatgatg agcaaatgga ggttgtaaag
    28081 ttaatataca ataaatattt agaacttagg tccttatcta agttatataa atatatctat
    28141 aataatggaa taagaggtcc tcgtggtggt aaatttgacc ctagttcttt atctagaata
    28201 cttagaaatc cagcatatgt aaaagctaat gatgaaattt tagaatacct taaaaataat
    28261 ggtatggata ttgtaggtac tccagataaa aaatgtggag tccttactta tgctaaaaat
    28321 acaactaact caatagcagc aattgcaaaa caccatggag tcatagaacc tgaaatatgg
    28381 cttgaagtac aaaatcaact agataaaaat cgtgataaaa ccccaaggat agcaactgga
    28441 aaaacagctc ttttatctgg ttttataaaa tgtggtaagt gtggttctaa tatgagaatt
    28501 acctataaag gtagaaaaac tgataaagat ttaaagtatt attatatttg tggcactaag
    28561 aaaacgcttg gcgctgataa ttgtaactgc aaaaacctta atggtccatt agttgaaagt
    28621 ttaattatag ataaaatcaa gaactgtaaa gaggaatcta tcataagtac attcaataat
    28681 aataacatta agactggtaa tatttttaat gttttaaaat ccgagactaa tttacttaaa
    28741 aatcaaattt tagataagga aaaacttatc aaaaatttag ttatagagct tgcaaaaaat
    28801 actgatagca ttgcttctga ttatatagtt tctcaaattg agaacttaaa tagtgagatt
    28861 aatattttaa aagataagct taatactttg gagtctagta atgccaatat tagtagtgat
    28921 agtattaatc ttgatattat tgttagtaat ttgaagagat ttaatgatga agttgataat
    28981 gccagtttag atgataagag attgttatta tctactatta ttaatagtat agtttgggat
    29041 agcaatttag gaaatcttaa tattatttat atgggtgctg aaatggataa ttctgtttct
    29101 ttaagcaatt agttgcactt ttttagctat agtagaagca ataagcatat cagataaggt
    29161 tgcaatacta tctaaaagac cagctaccat aaaaaatatt tacgatattg aacttaacat
    29221 ggatggaaca aaaacgcctc ttaaaacacg taaagttcct gattttcaaa agtatttcga
    29281 catattatgg aaggagctag atacacatga ggcttaataa aagatctgaa ggatatataa
    29341 actatgttaa aggtgtaaaa agagaaaaaa gaaatgtatt attctaccag ctattaattt
    29401 taattggttt tattgtaatc tgggaacttt tagctgactt aaatgttata aatacatttc
    29461 tatttagcaa accaagtgat atttacaatt tatttattca atatgcatct agtggtcaat
    29521 tatttaaaca tattggaata tctgtatatg aaactgttct tggtcttgtt ataggtactg
    29581 tattaggtat tttggtagca attgctcttt ggtggtcaga aaaattatca aaaatattag
    29641 accctttttt ggtagtttta aatgctcttc ctaagactgc tttagctcct ataattatag
    29701 tttgggtagg tgctggaata gaaggaatcg tcgtaacagc tgtaactatt tcggttgttg
    29761 ttactatact ttcagcttat aattatttta tgaatataga cgaagaaaaa atcaagatgt
    29821 taaagagttt tggagctagt aaatctcaaa tattatttaa actcatatta cctgcaaaca
    29881 caggaaattt aataaattta actaaaatta atattggtat ggcatgggta ggtgtaatag
    29941 ttggagaatt tttagtttca agatatggaa taggctattt gatagtatat ggttctcaag
    30001 tgtttaaatt agatttagta atgatgggag tatttgtatt ggctatctgt gcctgggcaa
    30061 tgtacgctgt agttaatatt atagaaaaaa tatacaattc cagataagta aaaattttat
    30121 ttagaatatt tgatgtctca gtgattataa taaaaaatga tttctttaaa gtaatcattt
    30181 ttttaatata aaaacttata ttaaatttga ttatcttttc ataagttggt aaatgtcgat
    30241 atatcctgta tctacaagga gtatcggcat tttctttttg gaagatactt tgcataagtt
    30301 ggcatttaac cgtataagaa tgccatcaaa agtagtaaat aagtagtaaa ctctagtcat
    30361 accactaggc agataaccgt tccatttcag ctcttgcaga actaaatgaa ccatgtgcat
    30421 aatagccaag cgtcatagta atgtttttat gccccattat atattgtaat atattaggac
    30481 tcattccttt gttcgccata ttggtacaga acgtatgcct aaaggtatgt ggcgtaatct
    30541 ttggtagctt gtcctcatga gtcttattat acttcttgac taacgctctt aacatgcttt
    30601 gataattggt tgcttacttt ggtaacccat cacgtttaag gaataagaag ttttgataaa
    30661 catcaacaat gattggtttt gctttagcgt tcttacgccg ttccacgata cgttcaaatg
    30721 attgataagc tctttctcag tcattggaat ttctcgctta ccctgtgtgg tctttggaac
    30781 ttctacataa tagccaatct tggtatcact caacaattga tggtctacca taatggcttt
    30841 atttttcata tccaattcta aagtcaaacc gcataattca gaaatgagta gtcctatttc
    30901 taagaggata acaacatcat cataatattt acaataagta ttatcgctct ccataaagga
    30961 taataagtcc atttcctgtt ctgttgttag agcgattttt ggttctgttg tatcttccaa
    31021 aacatcactt aactgaaaat caaatggatt ttttctaata tagtcgtctt gaattgccat
    31081 gaaaaacgac gctttcagtg aacgcttata attgtcaatg gatttataag cacacccatc
    31141 ttcactcatt cggatagccc agcttttagc gtctgataat ttaaccatat caatgctttt
    31201 cgcacctagt ggcttattgt tcagaatatt cataagataa tggcgtcctt taaatgctat
    31261 tgcgttcgaa ttaaactata tacatggcaa tcaatcatgt ttctattctt tattatggaa
    31321 ttgtgacagt attcttcaat cttaaaacca catttttcca atactttact tgaccttcta
    31381 ttgtaggaat atacattagc atgaattttg ttaaggttgt atttttcaaa ggcaattttg
    31441 catatttctt ttactgcatt tgttgttatc cctttacccc aatagttcct agcaagccag
    31501 taacctaata cagcattttt attaatagag gtatcttctt caaatgttag gctgatactt
    31561 ccaatagccc tttcttgata aaaaatagca tagctaagtt gtttaacatc tggagttgcc
    31621 aaacaaaaac taataaaatc tctagcgttt tgctccgtat aagggttggg gaagttgtca
    31681 cgaagattct ttgcaatttc tttatcattc gcaaactcaa ctaaatcggt aatataagca
    31741 taattccact ctataagttc catttttctt ctccttttat gaaacagcta caagaaatct
    31801 ttctaagcca ataccactta tcaaacagcg ttctttttta tttccaagca tttgataagt
    31861 ccagtcaaca aatccaccat caaccatttc aacggtatca tttccttgtt tgaccgataa
    31921 tttgaaattg atacctttat aataattatt atcaacatca tcattatgaa ttgataactc
    31981 tatattagga aaaataagtt tgatagtttc aaccattctg tcgaaaaatc cgtcattatc
    32041 tttgtatccg tttcttttgc gtagaataat agagaaacga acattttcta tttcacttaa
    32101 tatatcttta taatagttta agtgcttttc taacaacatt ttttcacaag tgtaagaccc
    32161 tgtatctata ccagatgaaa ccatacaata taagccaaaa tgtgaatata aacctttacc
    32221 tacaaaattt tgtgctctta ttgccctagc agtagtagcc atgtggatag gtatggtatt
    32281 attttcttct tttcttttta atttatcagc aataattatt gccaacatat ttgaggggtc
    32341 tgcaagtgtt tctgtccctc gtagagcact tactacgtta tattgatcta cggagccaaa
    32401 aacgctacaa ctacctaatg gagcagatgg agaaagcata atcgtcttga tatccatatt
    32461 gaatgctttt ctcaataatt ttgattccaa agtatgaatt tcttctggat ctatactgct
    32521 agggatagta aatctattta gttggtatga ctttagaata tcagttgcag ttaaagtatt
    32581 tgcttgtcta tcaaacactt ctaatagaag tgagttcaaa tctgattttg atagagctga
    32641 taacttatca actatattct tatccccaat tttatttgaa attctactta aaatatcact
    32701 catagttatc ctccttactg ttactttcaa aataattata gaatggttac aacaactaat
    32761 ctaggtcgtt tgaccaaaaa tgaaaaaaca ctgaatattt accattcaat gtttttttac
    32821 tttgggttta tttgattaaa tcttgaaaat actgtaatgc aaccctttca gcttcaacct
    32881 ttcggacaat aggactttgt gataattcct ctcttaactc aagatacagg ttgatagtag
    32941 cctgcaatgc attaacacag cttctatatt catgggtagc aaccgtcttt tctaatgctt
    33001 caatatattg gggtgcatcc aattcgattt ttctaactcc acgaggtaat ttacctaaga
    33061 gaagctgtat caatggggca agtactgatt gtcttaaaaa agataaaaaa tcaattgttt
    33121 caaatagttc tctacgtcca atcttagtgc aagcgtaatg aacccaaatc caaaatctat
    33181 cttcaatcca ctgtaagtct ggagttggga agtatgcttc ttttttagaa taaatttctg
    33241 ttatgcagtt gttttcttca tagagaatag ctgaatcttc aaccctatct agtaaaccat
    33301 ctaaggttgt aaacttaaaa tcaacatgaa gaacagggga atcatagagg gaaattatca
    33361 atcgaggttc tccaacgtgt tcgccagtaa aagcagaaac taatgttcca aactgttcca
    33421 caataagtaa tctctcttcg ataacttctt cataggattt atcatctgct acaatcagaa
    33481 aatccaaatc agaatattca tctaacgatt ttgtgatata agaaccgcta attgcaagtc
    33541 ctattatcct actatctctt ttggaaaatt ctaatacgtt ttgtatcatt tcctgttgtg
    33601 ttttagtcat aatataacac ctctcacttt cttttattag ctatcaatcc aacataatta
    33661 tgcataggag tattatataa tgaagaaata aaaaaaaata ggtcacttga cccgaaataa
    33721 aaaagtattg aataattatt atccaatgct tttttgaaat tatacttatg ttgtaggaag
    33781 attctttaat acctctcgct gattaattac actaaatctt ttgttttcat aagcaattag
    33841 atgttcttgt acacattcat ttatagcacg attaatgctt ctaattggag cgtttacttc
    33901 gctagcaagc tgttttttgg taagcagatt taattcactt ctataatagt gtaaggatat
    33961 acacttcaga agtcgttctt taacacttaa agatgataat tcttcaatta attcagcatt
    34021 tacaactaat ttactagata tttctcgaat cagaaatttt gtagcttcaa aatctccctt
    34081 tagccagttt aaaaaatcat ctctatgaag tttatataca ttacaatctg tgaaagcgac
    34141 aatttcaacg ggttttttcc catcattaaa ttgttccatt tctccaaaaa aactaccact
    34201 tctataaaga tgaatagttg agatgtttcc tttcgcattt aagatgaatg cttctgcaat
    34261 accgtcaatc ataagaatta catattcatt ttcttgctca gcaaaaagaa tagtctcttt
    34321 ctttttatac gtttttttaa caagcttctt tttaatatct tcattcataa aatcaattaa
    34381 atgcatattc atccctcctt accaaatatt tgattaaatt atatcatctt tcaattagtt
    34441 aggtaagata gtaaaaatat atttacatag aaactattcc attattattt tccagttgtc
    34501 atttttctgc aaagtaagct caaactggga aagctgtgta actttggttt ccttatcata
    34561 gaaaaggaac accttttttc gtgttccatg ttctattaaa tatttagttt tatttgatac
    34621 aatactgagg tgtttatacc gtatttttgc atttctagga cttatcaacg tatcaaggct
    34681 cattcggtag tagtaaatta gtagtaaaat ggctttgtta gtcctgtgta tatgcggata
    34741 ggtactgtgt tctaggggat aatcagattt ttagtatgtt ttttaaatta aaataaagcg
    34801 taaatttttg attatcttct tataaactta ccatgttgcc agtatgaccc tattttatgg
    34861 gttttactgg ctttttattg cctccaatta tacataaata cagttatttt tttattacct
    34921 atgcttaaat ttctataaaa gaaaaaacag ccaacttttt aatcaaaatt aactgtgttt
    34981 tatattcaat atttaatttt catttttgta ctccgttttg tgctccgttt gttagatagc
    35041 agagacctaa tgagtgctca cagtttagcc caaaatctga agtgagccaa ctatttgctg
    35101 tgattaaata gtttaccttg aaacttaaat ttatcttcta aaattatctt ttaaactgac
    35161 gatagcaaaa atatatgtat tctttcattg atatttctct actatgtaaa attcctatga
    35221 taacaaatat tatttctgca acagtaagat agcagtcttt taagtcgaaa ataaaaaagc
    35281 ttggtatctg caaaaaatct aaacttcctc cccaaaataa tttatctatc aagctacata
    35341 ttgtaccagc aagtccacaa gacataatta cttttactga atagctcgtt tgttcatttt
    35401 ttgatttgta aaaagcatat ccagatataa taactaggat aactaaaatg ttaaacaata
    35461 ccaataccca taaattagat agaataccaa taaaatttcc tccataagag agatttgtat
    35521 tttgaacagg attgaatctt aaaaattttc cagttatatc aatttcaaag cccattaaga
    35581 ataatcctat aagaactttg ctaatttggt ctaagaatat taaggatata actggaaaaa
    35641 caaaagactt tacttgtctg atatcaacac ctccttgcaa aagtcattca ctttttcctt
    35701 actcagtatc gttaaacccc aaaaaggaat ttcaatttat taatttcaca tcaataatta
    35761 taacatgggt aaattatcaa gtcatttctt ttctgatttc gtaatatatt tctactcgta
    35821 atcgttgtac aaattgttgc aaatcatgct atcagactta gcatttcttt gtttgcttct
    35881 gcctctgacg catgggcgta taagtccagc agtcttgctt tcacttaata cggttttggg
    35941 cttctgtcgt cctcaatgac ttcgcttagt tggaaatcaa aaagattctt ccttacatag
    36001 tcgtcctcga ttgtcatgta gaatgacgct tttaaggaac acttgctgta ttataaaaag
    36061 cagagttgct tcatattttc ttacgtgtca aaaagcttcc aataatcgcc cctataagga
    36121 caatcggtgt taatctgaca aatagacact caaacgcatg aaaagctact ccaaaaacag
    36181 cggtttcagg gttggtataa ttccaattca agtaaggact atttagtcct cctaagacca
    36241 taaaaactat tactatgact gaacataata aaataaataa ccaatgaaac atttttcttc
    36301 tcatattttt tctttgtaca agttgcaagt ttataatctc taatttttca gaaatcactt
    36361 ttaagttatc aacttccaac tcaacaatag tttctccaag taaagtgctt accggcgttt
    36421 caagtacctc tgatatagaa atcaacatat cagaatcagg aactgacaat ccttgctccc
    36481 atttagatat tgtttgtcgc actacattca attttatagc aagttcttgt tgcgaaagtc
    36541 cttttgactt tctgattgtt ttaatgtttt cgcttaacat tagggctaac cacctttctt
    36601 tcagttgtta ccgatattat aggcaaaact gcttgttgct tcaagcaacg cattttaaca
    36661 ttcaactgaa actcccacgc ctaaaggtgt gtggtttttt tagatactac atgaagttct
    36721 acaatactct taattataat aaatcataac ctacaacata ttctcactaa tcatattacc
    36781 taagactttt attaacaaaa tatctgtcga caccattaac attaatatta ggctcgtttc
    36841 tcaacctata tagtttttat atcttcatac tagatattaa tttataacca tttaatttta
    36901 attcttttaa cctatttatt tctttatcat gtaatatttt aaagttatca tatgttttaa
    36961 cacacaattc tctgttgatt gactttaaat tatcattaac attcattatt aaaaatgcat
    37021 aatagcaatc tctttgaata acatatcttc taagccttct atattaaata tttttcttct
    37081 atatctggga cagaatacaa agtgataatt aattaaactg acagttgttt gagttttcta
    37141 tatttattct ccatatactt atattaacat tatatgtgta tgtatcaata ctttttatac
    37201 actatttatg atatccatac cacatctaaa ggagtgggct ttccgaacta ttctgtaaat
    37261 ttcttatttt tcctttaacc gctttgctta tatatacagt gtttatacgg atattctcga
    37321 tttttaagcg ctttttgtaa taaaaataga tatgtccagt aattaaaact gtagaaatat
    37381 ttataaaggc ataagacaaa tttacctaca atttttagtg tttagattct aaaaaataaa
    37441 aataattata attgagttca taattttaaa acttattgaa tataattata tgtcggttaa
    37501 agacgtatga aaataaaagg aatttataag ttatgtgttg tatagatact gtatatgcag
    37561 catgtaataa atcttagcac tatttatatt ctatacaaaa agatatttga agggagatta
    37621 aaaatgggaa tattagaagg cgctgcaatg ataagagaga ttgcaattaa aatagctaaa
    37681 gaaaaaggta ttacagaaca aaaggcatgg cctgaagcgg taaaagaatt taaagaaaaa
    37741 tatgaacttg tattataaac tttgactgta aaactgtctc tattttattg agacagtttt
    37801 ttatattata acattcatat tatgaagaat aatatatata attttagatt ccttgtaact
    37861 ttaacttaaa gtaaaataat ttagaataaa aacagtatta ataaatttta tttgtcatat
    37921 aaatttgttt ttaatattat tttttaaggt ataaatccta tatattagat ataaattttg
    37981 gaggtatatg atttggataa aaaacttgaa aatagatgct catggcaaga atattttatg
    38041 agactgtgtg aaacagtagc tgaaagagga acttgtgata gagcttatgt aggagctata
    38101 attgtcaatt cagaaaacag aatagtgtct actggatata atggtagtat ttctggggat
    38161 aagcattgta gtgaagttgg tcatgaaatg agagatggac attgtattag aactattcat
    38221 gctgaacaaa atgctcttta ttattgtgca aaagaaggta tttcggttaa agattgttct
    38281 atttatgtaa ctcactttcc ttgtttaaac tgtactaagg ctataattca atctggaatt
    38341 aaacatatat attatagaac tggatataga atagatgagt atgctataaa attattgcaa
    38401 tcgagtaatg tcttatatac aaaattataa ctttaacact atttaaataa tattctatat
    38461 ttttacaaaa taaattataa taaaagtgta agatataaaa agaaacaaaa ttctaagtta
    38521 tgcatatatt aaatagaagt taaatttaat tgtaaaggag atgctaattt gaaagtagta
    38581 ataataccag ggcacacttt aactggaaaa ggtacaggag ctgttgggta tataaatgaa
    38641 agtaaagaaa caagaatact gaatgactta attgttaaat ggcttaaaat aggtggagct
    38701 actgtttata caggaagagt tgatgaatca agtaatcatt tagcagatca gtgtgctata
    38761 gcaaataaac aggaaactga tttggctgtt cagattcatt ttaattcaaa tgtcacaact
    38821 tcaactccag gaggaacaga aacaatatat aagactaata atgaaaaaac atatgcagaa
    38881 agagtgaaca ctaggctggc tacagtattt aaaaataggg gcgcaaagtc tgatgttaga
    38941 ggcctttatt ggcttaatca tactattgca ccagcaatat taatagaagt atgttttgta
    39001 gatagtaaag cagatacaga ttattatgtt aataataaag ataaagttgc taaattgata
    39061 gcagaaggta tactaaacaa atctatatca aattctcaag ggggaggtgt aaataaagtg
    39121 tatgaaaatg taatcgttta tacaggtgac gctgataaag tggctgctca gattttacat
    39181 tggcaattga aggattcttt aataattgag gcgtctagct ataagcaagg tttaggtaaa
    39241 aaagtttatg ttgtaggcgg agaagctaat aaacttgtaa aaggtgatgt agtaataaat
    39301 ggtgctgata gatatgaaac tgttaaatta gcattaaaac aaatagataa attataaaat
    39361 tatgattatt ttataaaaaa taaataagtc aggataaaga aaaaatgata agaacaaaag
    39421 ttgtagagtt aatagcaaca gtatgcagag aaaacaaacc acataaatgg gttgatgaaa
    39481 attatactcc ttatgataaa agtggtaaag ttgaattaat gtcaatagaa gatttaaatg
    39541 agttaatatc aagcaatgga aaagcagatt tactttatag ttgtagatta caaaaaattc
    39601 taaaagagat ttatataaat caaagtagag catcttatat gtcaggttgt ggattatttt
    39661 ggagttcgta ttgggatata cttgaagaaa aatttgaaga atggttatat aattcttata
    39721 tcttttttga tgaagatgat gaatatttag aaggaatgga agattttgag cttgaatgta
    39781 aagatgttct tatggatgta attgaaacaa caagcataga tatatatgtt caaatgataa
    39841 agaggaatat taccaattat taaaaacaca tagtgtcaaa ttaagctaat aaaacgacct
    39901 tataaatact attttaaggt cgtttaaatt atatctctta gacttctttt atattaaact
    39961 tattattttt tatctagaaa aatattttta attatagtca atatatcaac tataacatcc
    40021 atatttgatt taacactgtc tttagtaaca agaaaatctg ctgtaacttc agtaactacc
    40081 tctgttacat ctttcatatt ttctcctact tcgccaaaat tttctacaac actcggaaga
    40141 ttatttaaag ttgtatttat attttgagta ttttcattta acatagttcc aacagtattt
    40201 aaaatcttat gtagtttaac aagtacaata attaaataaa ctaaagcaat acaagcgagt
    40261 atagctaata ataaatatat tatacttata ttaatcgtta ttgtattgtt catattattc
    40321 ctcagtaaca ctaactggtt caacaatatc tattatttta tcttcctcac tagaattttc
    40381 aacacttttt ttgttatcta tatatttttt tatttttttc tttgattcta taaagtttcc
    40441 ttttttattc tctagcttct cagatacttt gccttttaca tctacagttt tcatatttat
    40501 cttttcagca atttgttgtg aagcatcttt tatatcttct cttgtctctt ttccagattt
    40561 aggagccaaa agtactccac tcactgcacc agtaacagct cctaatacta caccagctgt
    40621 agcaatttta gctttcttaa ctttttcagc tctctgttta gcttttcttt tatcttctat
    40681 tttctttgat agattcatgt ttaatcgccc cttattataa tatttctaaa cattataata
    40741 cattttttac cataatgata tagtttctaa ctaaattctt aaaaatcaat aaaaattctt
    40801 tttaattact tactatccat tacaatttaa atttttataa ggattttcta tatattttta
    40861 ttttattagt atataatatg tatcacatat agattataaa tatcttattt aattagtttc
    40921 atgtatataa ctaaaaattt tttagtataa atgaccttca atctttctat atagattaaa
    40981 ttagtatctc aaaaaacaat aagagaggaa ttaatcctct ctttttaatt taattagatt
    41041 tggcagggcg gcgtatcctg catctctatt aactactcta tagtagcgta ttgggtgcct
    41101 tttccaacct caaatctaac taataaatat ctacatgtat attaatatta taacctatat
    41161 gataatattt ttcaatattt tcggggaaat taataatatt atgttaacca tcatattgtt
    41221 ttactcttcc actgtttatt ctactttcta atttcgcttc ttttatagca tacattgaag
    41281 agacatataa atattctttt tttgtatcta attctgtagc cactaaaata taatcatcaa
    41341 ttttttttat aaactccaca cttttcccag gttttttagg atgacttcct atatattcag
    41401 gtgactttaa taccattttt atagtatcta ttatatcatt aaaaatatcc ttactcagtt
    41461 gacatctatg ttttttcttt atatgtttta taacaccaga agaagcataa attttaccag
    41521 tatattcaat tcctgcaagt tctgccgttt cttcatctat gattccaaca actttgtctg
    41581 tagacatata aagcacattc ctttctaaga taatttactg ttaatactgt aaaatttatt
    41641 taaattaaat atccatattt aaagtatagt ttattccaaa aagaaaagat agtataatat
    41701 agttttttca tatatgactt tatataatcc ctttttatat aatttataaa tggcaaataa
    41761 ggttctattt tcaaggtatt tttagaaact gtttaatttt tttaagtttt attgagtact
    41821 cttgcttctt tataaaaata taatctaata cctatattat ataaaacgtt ttcttttaaa
    41881 gttttctgtg ataatattat ttatatataa aataaatttc taataggagg aaatcatgaa
    41941 caatcaacaa caagaagtat ataaaaaatt agatgaatta aacatacctt atgaggctgt
    42001 aaatcatcca ccagtttata caattgaaga aatggaagaa ttaaaaacat tacatatgga
    42061 atttgttgta aaaaatttat ttcttcgtga tgctaaagga aaagaacatt ttttagttgt
    42121 attaggtaaa gacaaaaaag cagatttaaa agatattcgt caacaaattg atagtaaacc
    42181 tcttagtttt gcatcagaag aaagacttca aaaatacttg aaacttacta agggagctgt
    42241 aactcctttc ggtatattaa atgataaaga agctgttgta aaagttgtat ttgataagga
    42301 tttattaaaa atggataaaa taggtataca tccaaatgat aatactgcta ctgtattttt
    42361 aaaatttgaa gatatgaaaa agcttataga agaaaatgga aacgaaatat actacgtaga
    42421 agtataattg caacttaaaa aatggcaaaa gcaatgaatg cttttgcctt cttactactg
    42481 ctgtaatcaa tgctattaat ataatcattt ccattagtat aattattttc tacaataatg
    42541 attatttatg tttacttatc tttttttgca gatacaagta gcatcatagg tctacgtagc
    42601 tcatctttca taccaggaat tgcatctagc agtttttctt ctggttgagg ctcaataatt
    42661 tctaatactt caaatcctgt tttaattaaa gtatttaaat aagttgttaa tgttttatga
    42721 tattttataa cctcttctcc tagaaaattt gacttacgta atccttcttc aaaatatcta
    42781 tctactggcc agtgtagatt attaccatcc ttatcacagt gccactcctg acttccatgt
    42841 gcagtaaata taggatgttc aacagaaaat acaaaatcac ctttatttga aagacaatta
    42901 cttatatttt tacatatatc ttcaaatgat tgaatataat gaaatgctaa agaactaata
    42961 accacatcaa aactatcttt tggaaaatta atatcttcaa taggcataca tatgtatttt
    43021 atattatcaa atgttgtttt gtttctagct tcttttagca ttttttctga aatatcaatt
    43081 ccaacagcag aagttgcccc attctcaact gcatgttgac aatgccatcc aaatccacat
    43141 cctaaatcta aaactctttt tcccttgaaa ttaggtaaca tttttctaaa agtatgccat
    43201 tctccagcac ctgctagtcc atttatagaa cgttccattt tactgtattt attaaaaaat
    43261 ttatcatcat catatttatt ttgtttcata aagctccctc cattttaaat atatttattt
    43321 atcaatactc tatcaaatat aaacttttat cacttttaca tgaaataata aaagtttata
    43381 ta
//
LOCUS       DAFNNB010000014        42960 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0014, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000014 DAFNNB010000000
VERSION     DAFNNB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 42960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42960
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0014"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(332..1213)
                     /locus_tag="L3M60_000696"
     CDS             complement(332..1213)
                     /locus_tag="L3M60_000696"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBN6225393.1"
                     /translation="MLFKGSAVALVTPFTEDNNVNFEKLGELIEYHIENDTDALVVCG
                     TTGEATTMSESEIFAVIKYTVEKVNKRIPVIAGTGSNNTMLSVHMSQEAEKLGVDGLL
                     IITPYYNKTNEKGLKLHFETIANSVKLPIILYNVPGRTKVNIKPSVVAELAKIDNIVA
                     VKEASGDLAQVAEIAKLVPEDFAIYSGNDDTILPLLSLGGSGVISVLANICPKETHDL
                     VAKFFEGDIEGSKKLQLDMDALIAALFIEVNPVPVKTAMNILGFNVGDLRLPLAEMEE
                     TNLNVLKHKLTNFGFKF"
     gene            1536..2729
                     /locus_tag="L3M60_000697"
     CDS             1536..2729
                     /locus_tag="L3M60_000697"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBN6225394.1"
                     /translation="MDTLKELFKIGSGPSSSHTMGPQRAAERFKNENPDAESFRAILY
                     GSLAATGKGHLTDYIIEKTIAPKKVEIVWEEDIIKDFHPNGMKFEALDKDKNVTAEWT
                     VYSVGGGTIAEEGQRNSKSNSIYHLDTMDEIVKWCKENNKTLVDFVLECEPKDIKDYI
                     KTIKDAMRKSIDDGLSTDEIIPGKLLLKRRASTFYNAYKKDKSFSTLVYAYALAASEQ
                     NASGNIIVTAPTCGSAGVIPGIFFAMQDFYNYDDEKIIEALLVAGIIGNIIKTNASIS
                     GAEVGCQGEVGAACSMAAAAVAYLKGGTIDHIEYAAEIALEHHLGMTCDPVYGYVQIP
                     CIERNAMAAQRAYDAANYALLTDGSHSVSLDQVVETMKETGIDMMDKYKETAKGGLAK
                     HFFSC"
     gene            2934..4052
                     /locus_tag="L3M60_000698"
     CDS             2934..4052
                     /locus_tag="L3M60_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HBN6225395.1"
                     /translation="MREASYLQEQLYKHRKSLNPIAEIGRKEYKTSKYIRDYLDKLGI
                     EYEIYLETGIVGVIEGKRPKKDIAFRADIDGLHSEEGVKHLCGHDGHATILLGLIEYL
                     NDNKEDLNDNIVFIFQPAEEGPGGAKALIDEGVLRKYNVDEIYGLHIYPELPEGYVGT
                     REGYFMAQIGDIDIEIVSKSGHGAMPQNGIDGIVIASNFVNSLQTIVSRNISPIDNAV
                     LTIGRIEGGARRNIIAENIKMEGTMRCFSPEVYEKMKLRVRELARGFELAYNCKVNIN
                     IIDDYIAVKNDKDLYQEFVEAVGSDTVVELEPLMISEDFSYYQKEVPGLFFMLGSRNE
                     EKGFVNGLHNINFNFDEKICVNALNVYSALLKYKEAID"
     gene            4183..4428
                     /locus_tag="L3M60_000699"
     CDS             4183..4428
                     /locus_tag="L3M60_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HBN6225396.1"
                     /translation="MKEKVQIEKKITNGKNTKILTNDDIMKYEIASELGLMDKVGERG
                     WAGLTAKEAGRIGGMLTSRKKRLKKIVEEQNKKDESI"
     gene            4415..5161
                     /locus_tag="L3M60_000700"
     CDS             4415..5161
                     /locus_tag="L3M60_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBN6225397.1"
                     /translation="MSQYSDFAFVYDELMNEVDYDGWVKYIEDIIDSEGVKVKNILEL
                     ACGTGNLTIPLTKKDYDIAGIDISDEMLSVAKEKAEKEGVELVLLQQDISELDFEISD
                     LDCVLCACDGFNYITYDDDLENVFSKTYELLKQDGIFIFDISSFYKLANILGNNMYGE
                     NREDIAYMWQNYFDEEENIIEMELTFFIKDECGKFERFEEIHQQRAYTEDEIVDMLKE
                     SGFKDIKSYGDFTFDKPKEESQRIFFVCKK"
     gene            5445..6320
                     /gene="hslO"
                     /locus_tag="L3M60_000701"
     CDS             5445..6320
                     /gene="hslO"
                     /locus_tag="L3M60_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBN6225398.1"
                     /translation="MRDYVLRATSGNGQVRAFVATTRNTVEEARRLHETTKVATAALG
                     RTLTATSIMGLMMKNDSDKLTVIIKGGGPIGTIIATSDSKGMVKGYVGNPQVEVEDYP
                     NGKLNVAAAVGTEGVVKVIKDLGLREPYNGTYPLVSGEIAEDFTYYFAVSEQTPSVVA
                     LGVLTKEDEVEFAGGFIVQLMPDAEEETIAKLEENVAKLPSITNMLKEGKSPEDILNI
                     VLDGLEPKILDTCEVGFMCECSKERVKTALVAIGKKSLAQIIEEDKKAEVGCQFCNKK
                     YMYTEEELLEILKEM"
     gene            6710..7510
                     /locus_tag="L3M60_000702"
     CDS             6710..7510
                     /locus_tag="L3M60_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBN6225399.1"
                     /translation="MESKTKHIGISILILLMIFLIAKNFNGKLEIPIPESNSRKFKSA
                     AVFYPQHQDDEVLWGGSAIVDAIKQCGVDNVYVVLVSDGSGVNVFKANTKFRNLTRKQ
                     KEELRNNEFKSALRKLGVKPQNVIILADIDKKEGTHYELMKKTILDFEHKFKGNVTHI
                     AHHYKYDDHIMHRKNGEVLKKLKREHKIKDDLYFIKPKYVKYIPRNERAIYKAMEFDD
                     YNKVKSACYEYKVVNSKRKMYGIGYISAHSYFEHLLNDPELTSVLSED"
     gene            8037..10277
                     /locus_tag="L3M60_000703"
     CDS             8037..10277
                     /locus_tag="L3M60_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBN6225400.1"
                     /translation="MNNEFIILKGCKENNLNNISLKIPKRKITIFTGVSGSGKSSIVF
                     ETIAKESQRQLNERFSTFVRSFLPKYGEVKADCIENLSTPIIIDQSRLGGNSRSTLGT
                     ITDVNSFLRALYSRFGSEYIGNANMFSFNDIDGMCPHCHGLGKKLVPNMNQILDMNKS
                     LNEGAILLSGFGVGSWHWNIFSESGFFDINKRICDYSEVELEKFLHGKAEKIKIENAG
                     QTNMTYEGLMVKFNRLYLSREGEISEATKKKLSKLLIEDRCPICDGRRLNERVYKSLI
                     NGYNIADLTSMQIDELVEVIKSIDEPEAQPLIKGIIEKLNSIIDIGLGYLTLDRETSS
                     LSGGESQRIKMVKYLNSNLVDLMYIFDEPSVGLHPKDVYKLNNLLKKLRDKGNTIIVV
                     EHDPDVIKIADHIVDVGPKAGKYGGEVVYEGSYENLLTSGTLTGNALSKFLPIKESVR
                     EHRGYLEVKNCNKNNLKNVSIKIPKGVLTLVTGVAGAGKSTLIKDEFLKQNPNAVLID
                     QSPVSANSRSSLATYSGIMNNIRSIFSKTNGVNASLFSSNSEGACDNCKGSGIVEMNL
                     AFMESIKSTCNICEGKKFKKEVLEYRFQGKNIIEVLEMSVLDAIEFFNLKQIKTKLQC
                     IEKMGIGYLTLGQTLDTLSGGECQRLKLASELNKESSVYILDEPTTGLHMADIENFIG
                     IIEEIVDNGNTVIIIEHNIDVIKRADWIIEMGPEGGTKGGRVIFEGIPKQLCNNKISL
                     TAKYIV"
     gene            10690..12225
                     /locus_tag="L3M60_000704"
     CDS             10690..12225
                     /locus_tag="L3M60_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBN6225401.1"
                     /translation="MINDILTLLSERSDFFIKLSIEHLMISIISIIIAIVLGGIIGII
                     VSEFHNSSKPILGTINFLYTIPSISMLGFLIPFSGIGNTTAVIALTIYALLPMVRNTY
                     TGITNVDKNILEAARGMGSTDFQILYKIKLPLALPVIMSGIRNMVIMTIALAGIASFI
                     GAGGLGVAIYRGITTNNTVMTITGSILIALLALIFDFILGLVERFFQRKRKKKNRKKV
                     FAILGSLILIVTIFTSYLFNPSKTQTIHIATKPMTEQYILGEMLDILIEKNTGLKVEI
                     TQGVGGGTSNIQPAMENGEFDIYPEYTGTGWNMVLKKNELYTENMFSKLQSEYKNKLK
                     MQWTGMYGFSNSYALAVRKEVADKYNLKNISDLKDVSSKLTFGAEYDFYEREDGYTPL
                     CNTYGLNFKDTVDLDIGLKYQAINEGKIDVMPIFTTDGQYSVSDVIVLKDDKNFYPSY
                     QCGNVVRLEVLEENPELSKVFKMVGDILTDKEMAKMNYDVENNNLEPREVAREFLNKK
                     GLL"
     gene            12241..13002
                     /locus_tag="L3M60_000705"
     CDS             12241..13002
                     /locus_tag="L3M60_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225402.1"
                     /translation="MTTIIQFKNIKKQYNDKTIIDNLNLDIEKGEFLTVIGSSGSGKT
                     TLLKMINGLILPDGGNILINQTDIKNEDLIKLRRRIGYCVQGSVLFPHMTVEENISYV
                     PNLLSKKNKLEVKRAVDKWMEIVGLPNDMKVRYPSELSGGQQQRVGIARALASSPEIL
                     LMDEPFGAVDEITRKQLQKEIKEIHKKTGVTIIFITHDIYEALILGTKTLVLNHGVVQ
                     QYDTPENILNAPANQFVDQLLNIRKSIIDDENLKK"
     gene            13122..13373
                     /locus_tag="L3M60_000706"
     CDS             13122..13373
                     /locus_tag="L3M60_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HBN6225403.1"
                     /translation="MNVENELLKNLDRLHTTELGVVRIKKNLSLDTNDVVNWCKTKIE
                     SPNAIINRKGKNWYISIYDCIITVNAHSYTIITAHKEKK"
     gene            complement(13686..14336)
                     /locus_tag="L3M60_000707"
     CDS             complement(13686..14336)
                     /locus_tag="L3M60_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225404.1"
                     /translation="MNIVMINGSPKVNKNNSQYFLSEVKQLIQNDNKVFTFKVDNTSK
                     YPDALNAILNCDRLIIAFPLYVDAIPSHLLHFLEECEKFLKTQNKKPIPVYAIVNCGF
                     NEGEHNHLAIQMIEHWCKKINFTWIQGIGIGAGEMFGSLESVPLGKGQKKDLGIALKE
                     LSKNILSNNINIGNSKKNNIFISPNFPRFLYKLIGNHQWNTQAKSNGLTKSDILKK"
     gene            complement(14333..14836)
                     /locus_tag="L3M60_000708"
     CDS             complement(14333..14836)
                     /locus_tag="L3M60_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6225405.1"
                     /translation="MKLLIHDLTDEELEKLLPKLDDNIKILPNTKEIHKCIGCFGCWI
                     KTPTECVVKDYYSNMGKLINESDGIIIISRCCYGGFSPFVKNVLDKSINYILPYFVIK
                     DNMMRHKSRYDKHINLEAIFYGNCITESEKETATKLVNGNAINFNIKEHSVCFYENIQ
                     DIRGESI"
     gene            complement(14885..15400)
                     /locus_tag="L3M60_000709"
     CDS             complement(14885..15400)
                     /locus_tag="L3M60_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6225406.1"
                     /translation="MLEYIILGFLMEKELSGYDLKQIMSESTSYFFDASFGSIYPALK
                     RLETKGYIHYHEVIDGSKLKKLYSITNTGKEVFLEWLKKPINFSKTKQNHLVNIFFYK
                     YLPKEIIETNLKMLITEVELLLNKLSKQKIDVEENHCVDQYTYMYATMIYGVDYYNFV
                     INWCKDLLKNL"
     gene            15680..16207
                     /locus_tag="L3M60_000710"
     CDS             15680..16207
                     /locus_tag="L3M60_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6225407.1"
                     /translation="MHEQIDYCERKKIRTIDIKGENMRELLKWVSIINRYNTNYIDRK
                     LADIGINNSQYFYVLHICNNPGITQDTIFEKILVNPSNITRALANLEKEGFITREPGV
                     KDKRTWHLYPTEKSLECYDEILKITNGYVEELLSSFEKEEQELFMSMLKKVAFLAIDM
                     NEEAKLEAKENGRKI"
     gene            16191..17594
                     /locus_tag="L3M60_000711"
     CDS             16191..17594
                     /locus_tag="L3M60_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6225408.1"
                     /translation="MEEKYKEPLEYEKVSKLLTKYAIPSIIAMLVSALYNIVDQIFIG
                     QGVGVLGNAATNVTFPLTTICTAIALLLGIGGASKCSLELGAKNSEKATKAAGNSICL
                     MAVFGISLFIIVSIFLTPLLKFFGSTSEILPYAHTYTKITSIGLPFLIMSTGMSKLIL
                     ADGRPKASMFCMLIGAVINTILNPLFIFVFNMGIAGSALATVIGQLISFGISVHYIKN
                     FQHIKLHKKSFKLDSIIYKHIFALGSSSCFNQLAMTVTQIVMNNTLAYYGARSVYGSE
                     IPLACVGIIIKVNMIFISIIIGISQGMQPIIGYNYGAEKYNRVKEAYKFAVGSATIIS
                     VIAFLCFQFFPRQITSIFGNGNETYFIFAEHYFRTFLFLTFINGVQIVSSTFFTSIGK
                     SVLGLFTSLTRQILFLIPLIVILPMFMGIDGVMFAGPIADAAAAMVCIYFAVHEMKSL
                     TIKQNNLMSNHNKIAEV"
     gene            complement(17954..18265)
                     /locus_tag="L3M60_000712"
     CDS             complement(17954..18265)
                     /locus_tag="L3M60_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225409.1"
                     /translation="MKKIIKSFTFWFFLIALFEISMHQIGQDSKSIILIGFNPILSII
                     SRIDSFFIFMDSGMQIPCRTITGSISIYWYIASILSFLIYGIILDLIRIVISKIGNKT
                     K"
     gene            complement(18321..18914)
                     /locus_tag="L3M60_000713"
     CDS             complement(18321..18914)
                     /locus_tag="L3M60_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6225410.1"
                     /translation="MISDSISKRRSIRKYKNQSISHETIEKIIEAGINAPSSKNRQPW
                     RFVVITEKEKESMLKAMSKGIQNEIDDNGLLPGSRQHISGANYTVEIMKQAPVTIFIL
                     NILGKSLLENLSPEERFYEMANIQSIGASIQNMSLKAVELGLGSLWICDVYFAYRELC
                     EWLNTDSQLVAAISLGYPDEEPSRRPRLQLNDVTEWR"
     gene            19439..19669
                     /locus_tag="L3M60_000714"
     CDS             19439..19669
                     /locus_tag="L3M60_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225411.1"
                     /translation="MRLDMDSMLYVLIYMTLLVSVIGSIFYFCKDLLNHKIKYVLIKN
                     IFVNRTTYSEERLPKQYIVFQKYDIWRYCSLF"
     gene            19726..20376
                     /locus_tag="L3M60_000715"
     CDS             19726..20376
                     /locus_tag="L3M60_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBN6225412.1"
                     /translation="MDFISSILQGLLSILFNFTGDFGISIVLITLIVKLVLMPLSLKQ
                     RFSVKKQQELAEKMEHIKEKYKNDTKELEKQIQVNSVESMKSMLGCLIVLVQMPVVYA
                     LYHVCLDMTKEFTTIIIPWIANLGTFDNLFILPCIYTLIMLASNLVNYIPYLKINSQV
                     KFNKQMAMVTTITSLILTVRTPVAIGLYFITSAIYSLIEDICFRIYFNRKNRIVTK"
     gene            20578..21249
                     /locus_tag="L3M60_000716"
     CDS             20578..21249
                     /locus_tag="L3M60_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225413.1"
                     /translation="MAKILVVEDEKRMQSIIVEYMQKGGYTCVTADDGVEALTILKSN
                     NIDLMILDVMMPYLDGFSVCRVSREMTNIPIIILTAKGEEEDKLKGYEYGADDYITKP
                     FSPKVLLAKVNALLRRYTTGIPKNSLILGKIFIMVASRQVYVEDKLIDLTYKEFELLR
                     LFMENPNQVFSREKLLNCIWGYDFEGNTRTVDTHIKTLRKKLGSEGHHIVTLIRSGYK
                     FEVKE"
     gene            21246..22376
                     /locus_tag="L3M60_000717"
     CDS             21246..22376
                     /locus_tag="L3M60_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225414.1"
                     /translation="MSSLRKIIEKLKNLDISNWIKQNFGLHSVRKKVFLLSKLSGALI
                     ILFYLVVEELSINSKFEFWIWFIILVIFIMSIDFLLGKFISEPITSINKSAKSMSQLD
                     FSNPCTVNTNDEFGELSRSLNTMSTNLQQALSDLESANIQLEKDVNKERMLLEQRKEL
                     VDTISHEMKTPLGIIRAYTEGLMDEADEEKKKNYMNVIIEETDRMNNMIVSLLDLSAL
                     EAGVSKLNPERFDFIEFVETVAGRLLIDIPDANFYFTYDLPEYEVFVVVDKMRMEQVV
                     ENLIINAKKHVVYNGNLDLSITCENGLILFEIYNDGRIIEQDEISKIWSKFYRSVQSQ
                     RTGGSGLGLAIVSQILTMQGLEYGVKNRDRGVEFYFMIPIDE"
     gene            22824..23669
                     /locus_tag="L3M60_000718"
     CDS             22824..23669
                     /locus_tag="L3M60_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225415.1"
                     /translation="MIKVEELKFSYGKDKQILHGLNFEVKEGEIFGFLGPNGSGKSTT
                     QKILNGVLKGYNGHVSIFGNEVKAYTESLYQKIGVLFEFPYLYTNLSAIDNLEYFSSF
                     YPKNQRRDIYELLDLLEFKKEFINKPVSSYSKGMKQRISMARALVSNPKLLFLDEPTS
                     GLDPSGAVLFRKIIEEERKKGTTIFLTTHNMLDADLMCNNVAFIADGKIMAIDKPKNL
                     KMKNSNNKVEVEFVYNGSRDIKIVDIGELESGITFKYDEILSVHSKEATLEEVFIKYT
                     GRMLV"
     gene            23669..24697
                     /locus_tag="L3M60_000719"
     CDS             23669..24697
                     /locus_tag="L3M60_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225416.1"
                     /translation="MYSKFFTLVKKDFWMMIYGKFFVLTLGSLILYSCYINFGYVKFM
                     DEDSYHNNVYLYNPENISMSTSPLINQVSSIGELKAKLDEDTSAVCIDMSSSKPKVIL
                     HESIKGVDNYRADYALSLLSSEKDHNSKISDNNAIIQINDKDTSEIKQRKEMTCEILF
                     FEIVAIGFLGIASLLFKEKQMGVMRITGILPVKRSLFILSKIFAFLICDLCFTVLLVV
                     FNVGFFEGIKILSQVLVQASILSIIMSLIGFGCSMLLRDFKQFSLIYLVVAVFITTPI
                     FLSANTSFEIDWIKYHPFYHVYIQLKNAFFMNFSNELIYFVVCSLTIIVLFLLVKNIF
                     NKELVREG"
     gene            24700..25713
                     /locus_tag="L3M60_000720"
     CDS             24700..25713
                     /locus_tag="L3M60_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225417.1"
                     /translation="MIGLRYQLKSIRKDKMCIISFFLPVVMAILINFMGTIDMSSLGE
                     IHFGIVKDTLPVRTEQWLKKYGVLSVYDTKMHLIREIKDIKTDTIGVVRDGKGIKTVL
                     YGNEINAIKNVGKTLPEMFRLKGDLKDVEVTILPKDSILKEFQNVFIAMILITAMFMG
                     CTFNAMNIISEKEDGISFINEILPQSRSQYIIQKVFLGLLCGCLSSMITVFICLDISI
                     LDMIMMLLLVVFSAFISSLIGLFIGKLSEGLMVGIVYIKIVMILFIGVPLLIYLLGVN
                     TSGIGNLCYIMPSVATFNGIMEIINGNVIIIKDIVILAVHCVVWFMLYIILDKKRKLS
                     FSR"
     gene            complement(26246..27496)
                     /gene="cme"
                     /locus_tag="L3M60_000721"
     CDS             complement(26246..27496)
                     /gene="cme"
                     /locus_tag="L3M60_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Cme"
                     /protein_id="HBN6225418.1"
                     /translation="MENKNINSTWKKRVILFLASQYISLFGSSLVQMAMIWYVTLQTS
                     SGVWVTVLTICSFIPQMIISFFAGVWADYYNRKVLIIVSDSTIAISTLVLILFMMSSN
                     GTTSELIAISVISVIRSLGSGVQTPAVNAMIPQLVPEEHLMRFNGINGSLQSIVQFIV
                     PIVAAAILGFGNKINQVLLIDVFTAIIAIVLLIFIKIPKHDIPKQTEKVSFFADMVSG
                     IKYCFSDKFIRKVLFTYGAFMLLCVPSGFLIGLLIERTFGKGYIYLSIIEMIGFLGMV
                     LGGLIIGVFGGFKNRNKTFFLAILSYAIFSIILGLVTQAWQFFIFMFFTNFSIPIIQA
                     SAMTMLQEHVAPQMLGRVLSLPIIIYTGFIPLGMMIFGPMADVMSINNLIIVAGIILL
                     FFALAIPYSKQFYKQGISKSNESE"
     gene            complement(27582..28328)
                     /locus_tag="L3M60_000722"
     CDS             complement(27582..28328)
                     /locus_tag="L3M60_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6225419.1"
                     /translation="MDTYTTSEVADLIGVHPNTVRLYEELSLIPKPKRNTNGYRIFND
                     FHIEQFRFARTALKIEVVQRGLRKDALEIVRTSANRDFDKAICLVNQYLKQLEIEKSN
                     AEEALTIVTQILNGHTPETDKLFLTRKETAEHLNVTIDTLRNWEMNGLLKVKRKQNGY
                     RVYTDEDIKRLKIISALRCANYSLSAILRMLNALSSNPNTNIKTVLDTPKDDDEIISV
                     CDKLITSLKEAEHNAKTMKSHLQIMKKQFS"
     gene            complement(28443..29465)
                     /locus_tag="L3M60_000723"
     CDS             complement(28443..29465)
                     /locus_tag="L3M60_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HBN6225420.1"
                     /translation="MNKNNCLDLIFEKVDPSSVGMSSEKLSQLEPLIISQYSNINGII
                     IARKGKVVFEKYYNGYNADDTFHVASVTKSIISALIGIAIDKGYIKDVDQKVVDFFPE
                     YTCSPSEICKKAVTIRHLLTMTAPYPFKNMTESLERLCKQKDWIKYTLNQLGQKGEIG
                     TFKYSSAGTHLLSAIITKTTGKSAREFANEYLFKPIGMKIIPDYDMKDFGFEDFFGKN
                     LKGWAKDRQGYSTGGWGLTLSARDMLRFGFLYLNDGIWNNKRIISKSWIEESVKMNSN
                     EYGYLWWLCKKDGVLWYSAMGAGGNAICCIPQYDLVIAIPSKIVFKPRDRWEFIEKYI
                     LTSIIE"
     gene            complement(29465..31885)
                     /locus_tag="L3M60_000724"
     CDS             complement(29465..31885)
                     /locus_tag="L3M60_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBN6225421.1"
                     /translation="MKEIKINGAKIHNLKNIDVSIPKDKLVVATGVSGSGKSSLMFDI
                     VFEEGRKQYLQSLGILAGIDSEDKFDNIQGLAPTIAVQQNIIRQSNPRSTVGTRTNIL
                     NMLTLLYSVEGQIMCTMCDTPVSDNLICNNCGYEEERLKPSYFSYNSSDGMCMKCSGH
                     GAYFEINIEKLVLDKHDTLKQVLDRAKITPGYLRVFSKKFNDYLDIQFLQLPEEVKNE
                     VLYGHYENGKKSSSLSKVFHNRYKRGEDLNGVYTMTACSDCNGFKIGEEARRVLLNGK
                     HIGELGKMTILEIDEFLKILLDQGNLSTFGTNLVNNILSKTRHLIESRLGHLSLYREI
                     STLSGGEMQRLFLNSHLDSEMDSLIYVLDEPTAGLHESEKIDILKSIKKLKDLGNTVI
                     VVEHDKNTIEMAEHIIDIGPKAGIEGGQLVYQGNLEGLLQSNKSLTGQYLSGKYSMPN
                     RTSTKNITHIDEIPCITVQNANTNNLKNVTVSLPLGAMVGISGKSGSGKSSLISDTLL
                     PLLRSQFNNHTINNQTNSSESEIGDEDDYAVVETIADRLIGTKYISGYSEISQSPIGK
                     NMNSTPASYIGIWDKIRTLFAGQPESLERGFTAGHFSFNSKGACPKCSGSGYEKVWLG
                     NNLSIDHICSDCHGKRFNDESLSIKYKNKTIHDILNMSISEAVNFFKDIPNIVSHLNV
                     LEQIGMGYIKLGQPTPTLSGGESQRVKLAKEIGKKRNGNILYVLDEPTTGLSLYDTAK
                     LIQLLDELVANGNSVIVVEHDIDVLNVCDWIVELGPEGGDKGGYIIAEGSPKTLKENP
                     KSITGRYL"
     gene            complement(31971..32891)
                     /locus_tag="L3M60_000725"
     CDS             complement(31971..32891)
                     /locus_tag="L3M60_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6225422.1"
                     /translation="MTNAKSKKEYLNRICKVQDYIEEHLHEPLFLDELSNIAGFSKFH
                     FHRIFKAIEKETLAQYVNRLKLENATAFLIHRTDMTITDIAHYFGFTDSAVFSRAFKN
                     YYKISPIKYRNNYSKNCKEPYKISQYNRSISKPKCKNTREVKGEIEILELDNMNTIYT
                     RYTGSYGNLTSTFPKLLERLFTYASEQNLLGLENTQILAIYHDNPQFTQEHQLRTSIC
                     MTIPDNIVVKENDNLGNMIIPSGKYLVGHFDISKSEYSDAWDFMYNEWLSNVNYKLRA
                     SFPFEVYLNNPHNHPQNRHLVDIYLPIEPF"
     gene            33497..34411
                     /locus_tag="L3M60_000726"
     CDS             33497..34411
                     /locus_tag="L3M60_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBN6225423.1"
                     /translation="MSNSFYQLNSAVLYFNEKRDVNRIRFNHYVMRKENIQRLKGTLP
                     RGQFMMTVRKAASDLDVSTSTISRLVNEFIDLGILRLISRGVKGNCCSVYSYVCSENS
                     NTEILTGNSGIFKNIEKYLDVTINNNQKTMKYKHRDELFKNSNTNTYSRINEEHIDKG
                     YDSIIHTGYETENETKKKELLKNNLNKKNLNKNTFENSDEDLNYESPESIHEIIIRKL
                     NSESGKNFKADLSITKALIDKRLKEGYSLEDFYKVIEAKVRRWRYTDMEIYIEPTILF
                     GYEFESYLSEESSLSACNYGDMCYEDMY"
     gene            complement(34613..35233)
                     /locus_tag="L3M60_000727"
     CDS             complement(34613..35233)
                     /locus_tag="L3M60_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225424.1"
                     /translation="MFLINILLVDDHMLFSKSLSIALEEYEEIEQFYSTQDIHSLSEM
                     IQDKKIDIVLMDINLRGLSECDGLKVAYNLLETNSTIKIIILTGYDLPVYKHEAKKIG
                     IYGFLNKNISPDSLIDSLLKVYQGGTCFPSENTEVIIEDLTSMEKRILQLICSGKKRK
                     IIADELYISERTLSNHLQHIYDKLDVSSSVEAITKAIQMGYISPVY"
     gene            complement(35205..36788)
                     /locus_tag="L3M60_000728"
     CDS             complement(35205..36788)
                     /locus_tag="L3M60_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6225425.1"
                     /translation="MTNFNLKILFLGNYILSLLFMGISTSTKTYMNIQSIIPLIISTL
                     LLIDITYITSCNVRENNIISLFCGLLVLDSWYLLLSFVATPIGTIIFKILSTVIAYVS
                     IKFIFLFLFQGSGYKFRKITDILLLITCIGAIVGAFISNRTYACMYGIQFVVSIISFF
                     YIIFYHWKRVAFVIKSEKKPISISVFITIIGFLTYYFFTLRISNHIGNFGIYLTVLVF
                     FMSIHGIALKENDSFPISTVFNIKQRSIFLFISILVISLVVLFASGNFSIILIAINVL
                     FMLIFLCNIILEFNLRDEKNKVVKNSKYTFALEKLQQEEQLKTEFSNFLHDDILQNLL
                     SIKNMMSKSYRPEVQDIIYETLNNLNTCIRKQMQDYHPVILKNLTVKENYQNLIESVS
                     SSFPQKNICVSFKCSDTLFLVEPYDILIYRLIKELLTNVYKHSNGSHAWIALALEKNI
                     IKLNINDNGVKNTSFKTPLDITKITFDTIKHKGIISIKEQVENLGGTISISDNIPHGV
                     CVQITIPMKGDVSYKYFVS"
     gene            complement(37128..39155)
                     /locus_tag="L3M60_000729"
     CDS             complement(37128..39155)
                     /locus_tag="L3M60_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6225426.1"
                     /translation="MNIFYMAIKNLKKNFSFYSLYLLSVSFVITVFFAFTSFSVNKVM
                     LEKISTDGRVESMCNVISIFLMAFVIFYMAYSNRFFLRRRTKELGIYALLGYRKFTIL
                     SLLTFENILTCSGAFIIGLVLGALVHKSIVFGITALLNLSIDNSQIPFFNINAINKTI
                     IFIALVVLVLAISNGKFLYKTSLIKLIRYEKSTEKKLKFRKIPAFMGFIMILSGYCLS
                     LDILRGAKSVWISIGFYQIGLITMIIIVIGTVLFINSFLPYIMQKSKKNKRAFYTGTK
                     IITIPNFIYRIRSNSKTLIMLTLLSAATLTVSSVMALSLYYPIASVSRIAPSEIEFRV
                     EKNKQIEDVKQIVSQYMSNTDDIIFTQTNIYKVKSSSKDLPFEYEIGASKNNLSNSSS
                     SKEVISRESGFECISFSDYYTLLKAQNRTKLIKQLTPLRDNECILMKYQPNNDGYSEN
                     GTIYLLLINGKETPVTVRETSLNNAISFANSVGTLIVNDSIYEQIAQSKFPMTSILSI
                     NGKSIKNNENLYSNISDYLDGSPYLQGYSHRINDLLSINSSTFLLIGFLVVLFFIASG
                     SILYFNNISAISDSTSDYKILQKMGYTNIQIKKIIKKQVLPFFNIPFLLGIVDCIFAT
                     LVYKTALMQNLLGNSLSQYIPVIIAIFLTSVVYLIYYIFTIKSCCKIVLKK"
     gene            complement(39168..39914)
                     /locus_tag="L3M60_000730"
     CDS             complement(39168..39914)
                     /locus_tag="L3M60_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005340256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225427.1"
                     /translation="MNTILKVNSITKTYKGQQPSLDKVSFNVSEGEFIGIMGASGSGK
                     TTLLNVLSTIDKPTSGDIFINNINLKSLNDTNAADFRKDNLGFIFQEYFLLDSLTTFE
                     NISVPLTLQKLSPDKIEKMVKSLAERFGIASQLNKYPNELSGGQRQRTSAVRAIVKQP
                     SILFADEPTGALDSNSATELLHSLQEANKELKTTILMVTHDAYAASFANRILIFKDGH
                     IIKELNKQQNNRHTFYELIIQEIAKLDTKR"
     gene            complement(40205..41866)
                     /locus_tag="L3M60_000731"
     CDS             complement(40205..41866)
                     /locus_tag="L3M60_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp21"
                     /protein_id="HBN6225428.1"
                     /translation="MNKKNLAILISGIMFFSSSSSIFADNTTKQERLIGKTRYETAVE
                     VSKLGWVQSKTAIIVNGNSIQSALCANPFAKLKNAPILLVNNNSIESSTKAELKRLGV
                     DNVYIVDSGNSISSKVENEIKALNIKINKIVGNNIYEMSTNVLKEIDKIKKVENVAVV
                     NGLKGLSDAVSIASPAAIHNMPIILVSDENSNDSSVNFLNSRSIKKSYIVGGNKILSD
                     NAVKKYPNRERISGSDRNNTNAKIIDKFYKDKKINNVFVAKDGISQENALVDSLSIGV
                     LAAKENSPIMISNGSLVNSQKEFIENKDIDEITQVGGGYNQKAFEDILKLKNVNIRNK
                     TKKISLIQAKEILKQTLPGSDILVCYYDEDDNEYDCKVVKDNVLYEIEIDAYSGKIIE
                     IEKDDDLNDNNLGTKNISLDKAKAIALDAVPNGNLIYCKYDAEDNEYEAKVIKNKKTY
                     ELEISAFDGRIIDIDMDNDSDDDIDDNDNNHNNNSNTNDNSSIISAEKAKSIILDKVP
                     GGKIVKFKYDEDDKEYKGEIIKGKTEYEITINAITGKILEFEIDD"
     gene            42465..42902
                     /locus_tag="L3M60_000732"
                     /pseudo
     CDS             42465..42902
                     /locus_tag="L3M60_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
BASE COUNT        15765 a         5272 c         6792 g        15131 t
ORIGIN      
        1 aatattatat taacaatttt aattatatac aaaaaacgta caaaggtata ctttgtacgt
       61 ctaatttatc actcaaagta aaccctgtag ctcatcacct acaaaaataa gtgacagtat
      121 tacacatgtt atgtgcaatc ccagaaaata ttattgcaag aaatattttc ttcggctgta
      181 aatccttttt ccaatcatta acacctgcca gctactattg gatactctta gttacagcta
      241 cctctaccct aggttaatag gatattaaaa tttgattatt ctaataataa tacatattta
      301 agaaaaaatc aacttattta tataaatatt gctaaaattt aaatccaaaa tttgttaatt
      361 tgtgttttaa cacattaaga ttagtttctt ccatttcagc tagaggaagt cttaaatcac
      421 caacattaaa acctaaaatg ttcatggcag ttttaactgg aactggattt acttctataa
      481 ataaggcagc aattaaagca tccatatcaa gttgtaattt ttttgagcct tcaatatcac
      541 cttcaaagaa tttagctact aaatcatgtg tttctttagg acatatgtta gcaagaacag
      601 agataacacc acttccgcct aatgataata gtggtagtat agtatcatca ttaccggagt
      661 atatagcaaa gtcttcaggt actagtttag ctatttctgc aacttgagct aagtctccac
      721 ttgcttcctt cacagcaact atattatcta ttttagcaag ttcagccaca acagatggct
      781 ttatattcac tttagttctt cctggtacgt tgtataaaat aattggcaac ttaacactat
      841 ttgctattgt ttcaaagtga agtttaagac ctttttcatt agtcttatta tagtaaggag
      901 ttattattaa aagaccatcc acccctaatt tttcagcttc ttgactcata tgaacagaca
      961 acatagtatt gtttgaacca gtacctgcta taacaggtat tctcttatta actttttcaa
     1021 ctgtatattt tattacagca aaaatttcag attcactcat agtagtcgcc tcacctgttg
     1081 ttccacaaac aactagtgca tctgtgtcat tctcaatgtg gtattcgatt aattcaccta
     1141 atttttcaaa gttgacatta ttatcctctg taaatggggt aactaaagca acagcagaac
     1201 ctttaaataa catataatca aatctccttt tttatctaaa tttaattttt taagtacaat
     1261 aataattata gtacaaatta attaatttta ataggtatag ttaatatctt aactgtttaa
     1321 ataagatatt tatattatat taatattaga aaaattttaa ataaatgttt tattctcaaa
     1381 atatgcatat taatttaata aaatatacca aaatgggaat aaaaaataaa ttttatgaga
     1441 atttaataac taataattaa tatatttagg ttataataaa tatgtgcgta aaatctaata
     1501 ataagcaata atacagtaaa tttaggagga ataatatgga tactttaaag gaacttttta
     1561 aaataggaag tggaccttca agttctcata ctatggggcc acaaagagca gcagagagat
     1621 ttaaaaatga aaacccagat gcagaaagtt ttagagcaat attgtatggt agtttggcag
     1681 ctacagggaa gggtcactta actgactata taattgaaaa aactatagca cctaaaaaag
     1741 ttgaaattgt ttgggaagaa gatattataa aagattttca cccaaatggg atgaagtttg
     1801 aagctttaga taaagataaa aatgtaacag ctgaatggac tgtatattct gtaggaggag
     1861 ggactatagc agaagaaggt caaagaaaca gtaaaagcaa tagtatatat cacttagata
     1921 ctatggatga aatagtaaag tggtgtaaag aaaataataa gactttggta gattttgtct
     1981 tggaatgtga accaaaagac ataaaagatt atataaaaac tataaaagat gctatgagaa
     2041 aatctattga tgatggacta agtacagatg aaataatacc aggcaaacta ttattaaaaa
     2101 gaagagcaag tacattttat aatgcatata aaaaagataa gagtttctca acgcttgtat
     2161 atgcatatgc attagcagct tcagagcaaa acgcttctgg taatataatc gttacagcac
     2221 caacttgtgg ttctgctgga gtaataccag gaatattctt tgcaatgcag gatttctata
     2281 attatgatga cgaaaaaata attgaagcac tacttgtagc tggaataata ggtaatataa
     2341 taaaaactaa cgcttcaata tctggagcag aggtagggtg tcaaggtgaa gttggagcag
     2401 cttgttctat ggcagcggcg gcagtagcat acttaaaggg tggaactata gaccatattg
     2461 aatatgcggc agaaatagca cttgaacatc atttaggaat gacttgtgac cctgtatatg
     2521 ggtatgtaca aattccatgt atagaaagaa atgctatggc agctcaaaga gcttatgatg
     2581 cagctaatta tgcattatta actgatggct cacattctgt ctccttagac caagttgttg
     2641 aaacaatgaa agaaactggt atagatatga tggataaata caaagaaaca gctaagggtg
     2701 gacttgctaa acatttcttc tcttgctaaa aataaaattt tccaataata attcccctgt
     2761 tttgatttat gaaacagggg aattattatt aatggtgaat tatttaataa tgcaaaatat
     2821 ttttatattc atttagtttt ttatttttaa ttagtataac atgttatata atattttaat
     2881 tataaagaaa tatagaaata ttttttaata aattagaaat tggaggagat tacatgagag
     2941 aggcatcata tttacaagaa caactttata aacataggaa gtcattaaat cctattgcag
     3001 agattgggag aaaagagtat aagacttcaa agtatataag agattacctt gataaattgg
     3061 gaattgagta tgaaatttat ttagaaacag gtatagtagg tgtaatagaa ggtaaaagac
     3121 ctaaaaaaga tattgctttt agagctgaca tagatggtct tcatagtgaa gaaggggtaa
     3181 aacatttatg tggtcatgat ggacatgcta ctatattact tggattaatt gagtatttaa
     3241 atgataataa agaagactta aatgataata tagtatttat attccaacca gcagaagaag
     3301 gtcctggggg ggcaaaagct ctaatagatg aaggcgtgct tagaaaatac aatgttgatg
     3361 aaatatatgg gcttcatata tatccagagt taccagaagg ttatgtaggt acaagagaag
     3421 gctattttat ggctcaaata ggagatattg atatagaaat agtgtctaaa agtggacatg
     3481 gagctatgcc acaaaatggt attgatggta tagtgatagc ttcaaatttt gtaaactcac
     3541 tccaaactat agtgtctagg aatataagtc caatagacaa tgcagtgtta actattggta
     3601 gaattgaagg gggagctaga agaaatataa tagctgaaaa tataaagatg gaaggcacta
     3661 tgagatgctt tagcccagaa gtttatgaga aaatgaagct tagagttaga gaacttgcta
     3721 gagggtttga attggcttat aactgtaagg ttaatataaa tattatagat gattatattg
     3781 ctgttaaaaa tgacaaggat ttgtatcaag agtttgtaga ggctgttgga agtgatactg
     3841 ttgtagagtt agagccactt atgatatcag aagatttttc ttattatcaa aaagaagtac
     3901 caggattatt ttttatgttg ggttcaagaa acgaagaaaa aggatttgta aatggtcttc
     3961 ataatataaa ttttaatttt gatgaaaaga tatgtgtaaa tgcattgaat gtttattcag
     4021 cgttattaaa atacaaagag gcgatagatt agaaagataa attataaaga ttaaaaagta
     4081 gaaataccta aatttaaaaa aagtgtagtg ataaataccc cttacatatt tgtataaatt
     4141 tattttaatt gacaaaaata caaataagaa ggggtgattt ttttgaaaga aaaagtacaa
     4201 atagaaaaga agataactaa tggcaagaat actaaaatat tgacaaatga tgatataatg
     4261 aaatatgaga tagcctcaga actaggtctt atggacaaag ttggtgaacg tggatgggca
     4321 ggtcttacag ctaaggaagc aggaagaatt ggaggtatgc ttacatcacg caaaaagagg
     4381 ctgaaaaaga ttgtggagga gcagaataaa aaggatgagt caatatagtg attttgcgtt
     4441 tgtatatgat gaacttatga atgaagttga ttatgatgga tgggttaaat atatagaaga
     4501 tataatagat agtgaaggtg taaaagtaaa aaatatattg gaattggcgt gtggtacggg
     4561 taatcttaca attccattga ctaagaagga ttacgatata gctggtatcg atatatctga
     4621 tgagatgctt agtgtagcaa aagaaaaggc tgaaaaagaa ggagtagaat tagttttact
     4681 tcaacaggat atatcagagc ttgattttga aatatctgat ttggactgtg tactttgtgc
     4741 ttgtgatgga ttcaattata taacttatga tgatgatttg gaaaatgtat tttcaaagac
     4801 atatgaatta ttaaagcaag atgggatatt tatatttgat ataagttcat tttataaatt
     4861 agccaacata ttgggaaata atatgtatgg agaaaataga gaagatatag cgtatatgtg
     4921 gcaaaattac tttgatgaag aagagaatat aattgaaatg gaacttacat tttttattaa
     4981 agatgagtgt ggaaagtttg aaagatttga agagatacac caacaaagag catatactga
     5041 agatgagata gttgatatgc ttaaagagtc tggatttaaa gatataaaat cttatggaga
     5101 ttttacattt gataaaccca aggaagaaag tcaaagaatc ttttttgtat gtaaaaaata
     5161 agatattttt ggatataaaa gaaacttatt aaaaataagc attatataaa aatataaaaa
     5221 atgcagtttt atagattaaa aataaagtga tgatatttta tgatttatga tttttaatag
     5281 ttataaagtt ctatagatta aaattagaat aaaagaacta ttacctagta aaattaaaat
     5341 aaaagagcta ttacctacga ttaagataga taagtattta tagagtagaa ttaagatagt
     5401 aatatagtaa aatgagataa ttaacgaaat atggaggaat attgatgaga gattatgtac
     5461 taagagcgac atcaggaaat gggcaagtta gagcatttgt cgcaactact agaaatacag
     5521 tagaggaagc tagaagatta catgaaacta caaaagtagc aacagcagca cttggaagaa
     5581 cattaacagc cacatctata atgggattaa tgatgaaaaa tgatagtgat aaattgactg
     5641 taataataaa gggtggaggt ccaattggaa ctattatcgc cacatcagat tctaagggaa
     5701 tggtaaaagg atatgtagga aatccgcaag tagaggttga agactatcca aatggaaagt
     5761 taaatgtagc agcagcagta ggtacagaag gtgttgtaaa agttataaaa gacttaggtc
     5821 ttagagaacc atataatggg acttatccat tggtaagtgg agaaattgca gaagacttta
     5881 cttattattt tgctgtatca gagcagactc catcagtagt agctttaggt gttcttacaa
     5941 aagaagatga agtagagttt gctggaggat ttatagtgca attaatgcca gatgcagaag
     6001 aagagactat tgctaagtta gaggaaaatg tagctaaatt accttctata actaacatgt
     6061 taaaagaagg aaaatcacca gaggatatat taaatattgt tttagatggt ttggagccca
     6121 aaatacttga tacctgtgaa gttgggttta tgtgtgaatg ttcaaaagaa agagtaaaaa
     6181 ctgctttggt tgcaatcggt aaaaaatcat tggctcaaat aatagaagaa gataaaaaag
     6241 cagaagttgg ttgccagttc tgtaataaga agtatatgta tactgaagaa gagttgcttg
     6301 aaattttaaa ggaaatgtag actataaaaa taggtgcatt gatatagtag tatcatagtg
     6361 acatagtaat gtcttactta tataggaatt aaaatataat ttattaataa tagatatatt
     6421 tctattaaaa aataagttta ataaattttt aagcttaata aattttaaga attgagaagc
     6481 tttttagctg taatatttgc aatgttgcag ttagaaagct tttttatttt attatagttc
     6541 gtgtcaaaga ttgtatgaaa caataaaata ttgtaaaata atttgtatat ttaaacctag
     6601 ttgccacaaa ggtttataag atatttgaat tgattatgcg aaattctatg aagtagtaac
     6661 cctgtgactt taatcatggg agattcagaa gaaggagaaa attttaaata tggaaagcaa
     6721 aactaagcac ataggaataa gtatattaat attattgatg atatttttaa ttgcaaagaa
     6781 ttttaatgga aaacttgaaa tacctattcc tgagagtaat agtagaaaat ttaaatcagc
     6841 agcagtattt tatcctcaac atcaggatga tgaggtttta tggggaggaa gtgctattgt
     6901 agatgctata aaacagtgtg gtgttgataa tgtatatgtg gtattagtat cagatggttc
     6961 tggagttaac gttttcaaag ctaacacaaa atttagaaac ttaactagga agcaaaagga
     7021 ggaacttaga aataacgagt ttaaatcagc tttgagaaag cttggagtga aacctcaaaa
     7081 tgtgattata cttgcggata tagataaaaa ggagggtaca cattatgaat taatgaaaaa
     7141 gactatattg gattttgagc ataaatttaa aggtaatgtg acccatatag cccatcatta
     7201 taagtatgat gaccatataa tgcatagaaa gaatggggaa gttttaaaga aacttaaacg
     7261 tgaacacaag ataaaagatg acttatattt tattaagcca aaatacgtaa agtatatacc
     7321 aagaaatgaa agagctatat ataaggcaat ggagtttgat gactataaca aggttaagag
     7381 tgcttgttat gaatataagg ttgtaaattc aaagcgtaaa atgtatggta taggatatat
     7441 ttcggcacat agttattttg aacatctttt aaatgaccct gaacttacgt ctgtattaag
     7501 tgaagattaa tttcttataa gtagtaagtt atgtttgata agttgattag agttttctat
     7561 agtttcgaag gtattgaaaa tttagtttta tttaaaaatt taaagataga ggtttttaat
     7621 gtctggtatt ttaaagttaa gttgaataat tgtatagaat tatttgtgaa agaagaaaca
     7681 gtgtaaaaca tagtatctta tttatggttt taaaatcata ggtaagatac tttttttatg
     7741 taactaatac cattttgtga ttgttaactt aagagtttta tataatttaa cactcatata
     7801 taatgatgat atttatgtag aataaatgca attacgctta aataaaataa ctgtattttt
     7861 tatagaaatc aaaccctaca ctatactacc agataaaatc tacatgctat aatttgacct
     7921 gtgtttataa aaaaaggagt aatctaataa aatagtattt tatttatgta taactaacaa
     7981 ctattattac gatttactct aaagaattta aagaaaaata atgcaggagg tagaatatga
     8041 acaatgaatt tataatttta aaaggatgca aagagaataa tttaaacaat atttccttaa
     8101 aaataccaaa aagaaaaata actatattta caggggtgtc aggttctgga aaatcatcta
     8161 ttgtatttga gacaattgca aaagaatcac aacgccaact aaatgaaagg tttagtacat
     8221 ttgtaagaag ttttttacca aaatatggag aggttaaagc tgattgtata gaaaatctgt
     8281 caacaccaat tatcatagac caaagtagac ttggtggaaa ttcaaggtca actctaggta
     8341 ctattacaga tgtaaattct tttttaagag ctttatattc aaggtttggt agtgagtata
     8401 taggtaatgc taatatgttt tcttttaatg atattgatgg tatgtgtcca cactgccatg
     8461 ggcttggaaa aaagctagtt cctaatatga atcaaattct tgatatgaat aaatcattaa
     8521 atgaaggtgc tatattgctt tcagggtttg gagtgggttc ttggcattgg aatatatttt
     8581 ctgaatcagg tttttttgat attaataaga ggatatgtga ttattcagaa gtagaattgg
     8641 aaaagttttt gcatggtaaa gcagaaaaga ttaagataga aaatgctggt caaactaaca
     8701 tgacatatga gggattaatg gttaaattta atcgcctgta cctaagtaga gagggagaaa
     8761 tatctgaagc tactaagaaa aaattgagta aacttcttat agaagatagg tgtcctatat
     8821 gtgatggaag aagacttaat gaaagagtat ataagtcttt aattaatggt tataatatag
     8881 cagatttaac tagtatgcaa attgatgaat tggttgaagt tattaaaagt atagatgagc
     8941 cagaagcaca acctttaatt aaaggaataa tagaaaagtt aaacagtatc atagatatag
     9001 gtcttggata tttaacactg gatagagaga catcttctct atcaggagga gaatctcagc
     9061 gtataaagat ggttaagtat ttaaatagta atcttgttga tttaatgtat atttttgatg
     9121 aaccaagtgt aggacttcat ccaaaagatg tgtataaatt aaataatctt ttaaaaaaat
     9181 tgcgtgataa agggaataca attatagttg ttgaacatga cccagatgtt attaagattg
     9241 cagaccatat tgtagatgta ggaccaaagg caggtaagta tggtggagaa gttgtgtatg
     9301 aaggcagtta tgaaaatcta cttacaagtg gtacgttgac agggaatgct ttaagtaaat
     9361 ttcttcctat taaagaaagt gtaagagagc atagaggata tttagaagtt aaaaattgta
     9421 ataaaaacaa tttaaaaaat gtatctatta agatacctaa aggcgtattg actctggtta
     9481 ctggagtagc aggagcaggt aaaagtacgt tgattaagga tgaattttta aagcaaaatc
     9541 ctaatgctgt acttatagac caaagccctg tttcagcaaa ttcaaggtcg agtttggcaa
     9601 catatagtgg aattatgaac aacataagaa gcatattttc aaaaacaaat ggcgtaaatg
     9661 cttctttatt cagttcaaat tcagaaggag cttgtgataa ctgtaagggt tctggaatag
     9721 tagaaatgaa tcttgctttt atggagagca taaagagtac ttgtaatatt tgtgaaggta
     9781 aaaaatttaa aaaagaagtt ttagaatata gatttcaagg gaagaatata attgaagttc
     9841 ttgaaatgtc tgttttagat gcaattgagt tttttaattt aaaacagatt aaaacgaaat
     9901 tacaatgtat tgaaaaaatg ggtattggat accttacttt aggacaaact cttgatactt
     9961 tgtctggagg agagtgtcaa agattaaagc ttgctagtga attaaataag gaaagttcag
    10021 tgtatatatt agatgaacca acaacaggtc ttcatatggc tgatattgag aattttatag
    10081 gtataattga agaaattgta gataatggaa atacggtcat aataattgaa cacaatatag
    10141 atgtaattaa aagagctgat tggattattg agatgggacc tgaaggagga acaaaaggtg
    10201 ggagagtaat atttgaaggt attccaaaac aattatgtaa caataaaata tcattaacag
    10261 caaaatatat agtataataa ataaaatata ataaatttat aatgatattt taataaatat
    10321 aatgggtgta taaaatgata attaaactta acagtaataa ataatgtgag aaatattcaa
    10381 atttaattaa ataaagtgtc agaaatttgt ttaaatttta gcatttcaac agttctgacc
    10441 ttttttctta tttttatgga gaatatcgag aatggagtct ctaatagtta ctttttttgt
    10501 ctacttgcaa gtttgattaa aaaatgtcta tgcacacttg tgactttagt catggaaggc
    10561 tcagaatata atcttaatat ataatttgtg aaataatttg attacgattt gctgatgtat
    10621 aaacaataaa gattaactac attaaaattt aaatcttgtt aagtaaaaca tataaaagga
    10681 gagagatgaa tgataaatga tattctaaca ttattatctg aaagaagtga tttttttata
    10741 aagctatcca tagaacattt gatgatttct attatttcta taataattgc gattgttttg
    10801 ggtggtataa ttggtattat tgtaagtgaa tttcataatt cttctaaacc tatattggga
    10861 actatcaatt ttctatacac cattccatct atttctatgc taggatttct gataccattt
    10921 tctggaatag ggaatactac tgctgttatt gcacttacca tttatgcatt attacccatg
    10981 gttagaaata catatactgg aattaccaat gtggataaaa atattcttga agcagctaga
    11041 ggaatgggaa gtacagattt tcagatattg tataaaataa aacttccatt agctctacct
    11101 gttattatgt ctggtattag aaatatggta attatgacaa ttgcattagc tggtatcgct
    11161 tcatttatag gtgctggagg cttaggtgta gctatatata gaggaatcac gacaaataac
    11221 acggtaatga caattactgg aagtatactt attgctctat tagctttaat atttgatttt
    11281 attttagggc tggtagaaag attttttcaa cgaaaaagaa aaaagaaaaa tagaaaaaaa
    11341 gtttttgcca ttctaggaag ccttattctt atagtaacta tctttacatc ttatttattt
    11401 aatccttcaa agactcaaac tattcacatt gccacaaaac ctatgactga acaatatatt
    11461 cttggagaga tgttggatat tttaattgaa aaaaacacag gtctaaaagt ggaaattaca
    11521 caaggtgtag gaggaggaac atctaatatt cagccagcta tggaaaatgg agaatttgat
    11581 atttatccag agtatacagg tacaggttgg aatatggtac ttaagaaaaa tgaattatat
    11641 actgaaaata tgttcagtaa actacagtca gaatacaaaa ataaacttaa aatgcaatgg
    11701 actggtatgt atggtttttc taatagctat gcattagcag ttagaaaaga agttgcagat
    11761 aaatataatt taaaaaatat atccgactta aaagatgtat cttctaaatt gacatttgga
    11821 gctgaatatg acttttatga gagagaggac ggatatactc cattatgtaa tacatatgga
    11881 ctgaacttta aagatacagt tgatttagat attggattaa aataccaagc tatcaatgaa
    11941 ggtaagattg atgtaatgcc tatatttaca actgatggtc agtatagtgt ttcagatgtt
    12001 atagttctaa aagatgataa aaatttctat ccatcttatc aatgtggaaa tgttgttcgt
    12061 ttggaggtgt tagaggaaaa tccagagcta tcaaaggtat ttaagatggt tggagatatt
    12121 ttgactgata aagaaatggc taaaatgaat tatgacgtag aaaacaacaa tttagaacct
    12181 agagaagttg ctcgtgagtt tctgaataag aaaggtcttt tataaattgg aggaaatgta
    12241 atgacaacta ttatacaatt taaaaatatt aaaaaacaat ataatgataa aactataata
    12301 gataatctta atttagatat agaaaaagga gagtttttga ctgtcattgg ttcgtctggt
    12361 tctggtaaga caacactctt aaaaatgatt aatggtttga ttttgccaga tggaggaaat
    12421 attttgataa accaaacaga tatcaaaaac gaggatttaa ttaaattaag aagaagaata
    12481 gggtactgtg tacagggaag tgttcttttt cctcatatga ctgttgaaga aaatatttca
    12541 tatgtaccaa atcttctaag taaaaaaaat aaattagaag taaaaagagc tgtagacaaa
    12601 tggatggaaa tagtgggatt gccaaatgat atgaaagtac gttacccatc agaattgtca
    12661 ggtggtcaac aacaacgtgt agggattgca agagccttag cttcttctcc agaaatttta
    12721 ttaatggatg aaccttttgg tgcagttgat gagattacac gaaagcaatt acaaaaagag
    12781 ataaaagaaa ttcataaaaa aacaggagtt acgattatct ttattacaca tgatatttat
    12841 gaggctttga ttttgggaac aaagactttg gtattaaatc atggagttgt tcaacagtat
    12901 gatactcctg agaatatttt aaatgctcct gctaatcaat ttgtagacca gttattgaat
    12961 ataagaaaat ctataataga tgatgagaat ttaaaaaaat aaatttaaat ttaaaatcaa
    13021 attgttgtga taaaagctat tgacctagag ttaacttcaa gttgtatatt ggtactaaat
    13081 aatagattta attttgcaaa ggataaggtg ttttaataaa aatgaatgta gaaaacgaat
    13141 tacttaaaaa cttagataga ttgcatacaa cagagttagg tgttgtaaga attaagaaaa
    13201 atctttcttt ggatacaaat gatgtagtga actggtgtaa aactaaaata gaatctccaa
    13261 atgccattat taatagaaag ggaaaaaact ggtatataag tatatatgat tgtattataa
    13321 cggtaaatgc tcacagttat acaatcatta cagctcataa agaaaagaaa tgaaatgaat
    13381 tgcaaaatac aaaggtcaag agcattttcc attgtttgta gtacctatga agaagtatat
    13441 aaaataatat tacttttgag taataattta aattttggag attgaaaaat tgaatatact
    13501 gatttacatc agtaccttgt tggagcgact tggattttac aaaaagaata tgataaatat
    13561 tagactagat aatcaatgtt gtagtgaatt attccatgtc tagtgaatat aaataacata
    13621 aaaaaccgtc aagatgaata gtttattcgt gcttgacggt ttttagtatt tgttgaagta
    13681 aaattttatt tctttagtat atcactttta gtcaaaccat ttgatttagc ttgagtattc
    13741 cactgatgat tacctataag tttatatagg aatctaggaa aattaggtga aataaaaata
    13801 ttatttttct tggaattacc tatatttata ttattactaa gtatattttt tgaaagttct
    13861 tttaatgcaa ttcctaaatc ttttttttga cctttaccca aaggtacaga ttctagtgaa
    13921 ccaaacatct caccagctcc aattccaata ccttgaatcc atgtaaagtt gattttttta
    13981 caccaatgct ctatcatttg aattgccaaa tgattatgtt caccttcatt gaatccacag
    14041 ttaactattg catacactgg aataggtttt ttattttgtg tttttaaaaa tttttcacat
    14101 tcctctaaaa aatgaagtaa atgagatgga atagcatcta cataaagagg aaatgcgatt
    14161 attaatctat cacaattaag tatggcatta agagcatcag gatacttaga tgtgttatct
    14221 actttaaatg taaaaacttt attgtcattt tgaataagtt gttttacttc ggataaaaaa
    14281 tactgtgaat tatttttatt tacttttgga ctaccattta tcattactat attcatatgg
    14341 actcacctct tatatcttgt atattttcat agaaacaaac agagtgttct tttatattga
    14401 aatttatagc atttccattt actaatttcg tagcagtttc tttttcagat tctgtaatac
    14461 aatttccata gaatatagct tctaaattta tatgtttgtc gtatctactt ttatgacgca
    14521 tcatattatc ttttatcaca aagtatggaa gaatataatt aatactttta tcaagtacgt
    14581 ttttgacaaa agggctaaac cctccgtaac agcatctgct tattataata attccatcag
    14641 attcatttat aagtttaccc atgttgctgt aataatcttt tacaacacac tcagtaggag
    14701 ttttaatcca gcaaccaaaa caacctatac atttgtggat ttcttttgtg tttggaagta
    14761 tttttatatt gtcatcaagt ttgggtaata gcttttctaa ttcttcatca gttaaatcgt
    14821 gtattaaaag cttcataaaa tccatctcct tttattatgt atgtttactt ctaaaaatca
    14881 tatcttataa atttttcagt aaatctttac accaattaat tacaaaatta taatagtcaa
    14941 ctccataaat cattgtggca tacatatatg tatattggtc aacacaatga ttttcttcta
    15001 catcaatttt ttgtttactt aatttattta gaagtaattc tacttcagtt attagcattt
    15061 ttaagtttgt ttcaattatt tctttgggta gatacttgta aaagaatata ttaacaaggt
    15121 ggttttgctt tgttttagaa aaatttatag gtttctttaa ccactcaaga aatacttctt
    15181 taccagtatt agttatggag tatagttttt ttaatttgct accatctata acttcgtgat
    15241 agtgtatata tccttttgtt tctaatcttt taagtgcagg atagatactg ccaaagctag
    15301 cgtcaaaaaa gtacgaggta ctctctgaca ttatttgttt taagtcgtat ccacttaatt
    15361 ctttttccat taaaaatcct aaaataatgt attctaacat gttgcctcct agtatcaatt
    15421 agatatatat caattcgata tatttattat tgcagataat aaatattacg tcaagatgct
    15481 ttttaaaata agctaaaaag aattagaaat tattaataaa gatgtaaaaa aattaatata
    15541 aaaggtaatt aaatatcaat gaagatttaa aaatagttaa atcaaagtaa gagtctaata
    15601 tataaaattt atagtaactt tatggtttaa atataaacct gtgtgctatt gacaaagtat
    15661 aaaattagca gtaaaatgat tgcatgagca aatagattat tgcgaacgca aaaagataag
    15721 gacaatagat attaaaggag agaatatgag agagctatta aaatgggttt ccattataaa
    15781 tagatacaat acaaattata ttgacaggaa attggcggat attggaatca acaatagcca
    15841 atatttttat gtattacaca tatgtaataa tccaggtatt acacaggata ctatatttga
    15901 gaaaattctt gtgaatccta gtaatataac aagagctttg gcaaatttag aaaaagaagg
    15961 ttttataaca agagaaccag gtgtaaagga taaaagaaca tggcatttat atcctacaga
    16021 aaaatcttta gaatgttatg atgaaatact aaaaataaca aatgggtatg ttgaggagtt
    16081 gttgagttca tttgagaaag aagagcaaga gttgtttatg tcaatgttaa aaaaagttgc
    16141 atttttggct attgatatga atgaagaagc aaaattggag gcgaaggaaa atggaagaaa
    16201 aatataagga gccattggaa tatgaaaaag tttcaaagtt attaaccaag tacgcaattc
    16261 ctagtattat agcaatgtta gtaagtgctt tatataacat tgttgaccaa atatttattg
    16321 ggcaaggtgt tggtgtattg ggtaatgctg ctacaaatgt cacttttccg ctaacaacaa
    16381 tatgtactgc aattgcatta ttgttaggaa ttggaggagc gtctaagtgt tcattggaat
    16441 taggtgctaa aaattcagaa aaagctacaa aagcagcagg aaattctata tgcttgatgg
    16501 ctgtttttgg aataagttta tttataatag tatcaatatt tttaacacca ctattaaaat
    16561 tctttggctc tacatcagaa atactacctt atgcacacac atatacaaaa attacatcaa
    16621 ttggattacc atttcttata atgtccactg gaatgagtaa gttgatactt gcagatggaa
    16681 gaccaaaagc ttctatgttt tgtatgttaa taggagcagt tattaataca attttgaatc
    16741 cattattcat ttttgttttt aatatgggaa ttgctgggtc tgctttagca actgttattg
    16801 ggcaacttat atcttttgga attagtgttc attatataaa gaattttcaa catattaaat
    16861 tacataagaa aagttttaaa ttagatagta taatttataa acatatattt gcactaggtt
    16921 caagttcatg ttttaaccaa ttagcaatga ctgtaacaca gatagttatg aataatacat
    16981 tggcttatta tggggctagg tcagtttatg gaagtgagat tccacttgcc tgtgttggta
    17041 tcattattaa agtaaatatg atttttatat ctataattat tggtatatca caaggtatgc
    17101 aaccaattat agggtataat tatggggctg aaaagtataa tcgtgtaaaa gaagcgtata
    17161 agtttgcagt gggaagtgca actattatat ctgttattgc attcttatgt ttccaatttt
    17221 tcccaagaca aattacgtct atatttggta acggaaacga gacttatttt atatttgcag
    17281 aacattattt tagaactttc ttgttcttaa catttataaa tggtgttcaa atagtatcat
    17341 caactttctt tacatcaatt ggtaaatcag tgttgggact ttttacttct ttgactcgtc
    17401 aaattttatt tttaattcca ttgatagtga ttttaccaat gtttatgggg attgatggag
    17461 taatgtttgc aggaccaata gcagatgcag cggcggcaat ggtatgtata tactttgcag
    17521 ttcatgaaat gaaatcattg actataaagc aaaataattt gatgagtaac cataataaga
    17581 ttgcagaggt ataagacaat atttaagaag tatagaaaat taatttaaga agtataataa
    17641 gctaatttaa agaagtataa tgagctaact taagaaatat agaaaattaa tttaaagaaa
    17701 tataataaat taatttagga aatatagaaa attaatctaa agaagtataa taaactaatt
    17761 taagaaacat agaaaattaa tttaaagaaa tacaataaat taacttttaa gtaacattaa
    17821 attaatgtaa gtaaaataac gttcatgtga agatttaaag gtgcattttc tggagctaag
    17881 tatatgttgt agagtgtata tctctctagt ctgtgaagtt taatagctca agaaaatgca
    17941 ctatttatga tgcttatttt gttttatttc caattttaga aataactatt ctaattaaat
    18001 ctaaaattat accataaata agaaaagaaa gtatagatgc tatgtaccaa taaatcgaaa
    18061 ttgaccctgt aattgttcta catgggattt gcatacctga atccataaaa ataaaaaaac
    18121 tatcaattct ggaaataata gacaaaatag gattgaaacc tattagaata atactttttg
    18181 agtcttgacc aatttgatgc atagaaatct caaacaatgc aattaaaaaa aaccaaaatg
    18241 taaaagattt aataattttt ttcatcaaaa tacctcacct aatataaatt ctgtttgcac
    18301 tatttgctta ctataactat ttatctccat tcagttacat catttaattg taatctaggt
    18361 cttcttgatg gttcttcatc aggatacccc aaagatattg ctgccactaa ttgactatct
    18421 gtattcaacc attcacataa ttcacgatat gcaaagtata catcacaaat ccaaagacta
    18481 cctagaccta attccacggc tttaagcgac atattttgaa ttgaagctcc aattgattga
    18541 atgtttgcca tttcatagaa tcgttcttca ggggatagat tttctaaaag tgacttacct
    18601 agtatattta aaataaaaat tgttactgga gcttgtttca tgatttcaac cgtgtaattt
    18661 gcaccagaga tatgttgacg acttcctgga agaagcccat tatcatcaat ttcgttttga
    18721 atacctttgc tcatggcttt aagcatagat tctttttcct tttcagtgat gacaacaaaa
    18781 cgccatggtt gcctgttttt agatgaaggt gcatttattc cagcttcaat tattttctct
    18841 atagtttcat gtgaaatgct ttgattttta tattttctga tacttcgtct ttttgatata
    18901 gaatcactta tcattttaag taacctccgt ttattttttg gaaaaatata tttgttttct
    18961 atttttaatt attttagcat taatcagaaa aaaagtcatt gattttagta tacaattatt
    19021 aatgattgta ttaagaattg catcaaaaag tatgtgatta tactaaggga ttatattaga
    19081 cattatattt aaaattgtat taaaggttgt atttaatatt atattaagat ttatattata
    19141 ttgggtctta tattaaatta aggattataa attgaaaata agctataaaa actcaatatt
    19201 gttttgttca gagataaaaa aagaatgcct taggtgattt ttattaaaaa ttaagagtga
    19261 taagattata tactttataa atgtaaatat gacgaataaa aatgataaat ataataatct
    19321 atatatttac attaggagtt ttctatacta taattattta aaactaaata taaatgttgg
    19381 aattatttag gacttgaaat tgtttaaaaa taaatataag tgatgaagag gtgatgtgat
    19441 gagattagat atggatagta tgttatatgt actaatttat atgacattat tggtatctgt
    19501 cattggaagt atattttatt tttgtaaaga cctcttaaat cataaaatta agtatgtttt
    19561 aataaaaaac atatttgtaa atagaactac atattctgaa gaaaggttgc ctaaacagta
    19621 tatagttttt cagaaatatg atatttggag gtattgtagt ttgttttagt ttgaaatgaa
    19681 tctattataa attctaaagt ttaaaaataa aagggagaga ataaaatgga tttcatttca
    19741 agtatattgc aaggattatt aagtatatta tttaatttta caggtgattt tgggatttct
    19801 atagtactta taactttaat agttaaatta gtacttatgc cactatcatt aaaacaaaga
    19861 ttttctgtaa aaaaacagca agaattagct gaaaaaatgg aacatataaa agaaaagtat
    19921 aagaatgata ctaaagaact ggaaaagcaa atacaagtaa attctgtaga aagtatgaag
    19981 agtatgctgg gatgtcttat agttttagta caaatgccag tagtatatgc tttatatcat
    20041 gtatgtttag atatgacaaa ggaatttaca acaattataa ttccatggat tgctaattta
    20101 ggtacatttg acaatttgtt tatattacct tgtatatata cgttaattat gttagcttca
    20161 aatttagtga attatatacc gtacttaaaa ataaattctc aggtaaagtt taacaaacaa
    20221 atggctatgg tgactactat aacaagtctt atactgactg ttagaactcc agtagccata
    20281 ggtctgtact ttataacaag tgcaatttat tcattaattg aagatatttg ttttagaata
    20341 tattttaatc gaaaaaatag aattgtgaca aaataatata attattatta gaaatatttt
    20401 agcctgctaa cataggaaaa gttagtaggc tatttttatc tatgatatca attccattaa
    20461 gaatagtcta aaaaatacta tcaaaatact atccaaatac tataaaaaaa tagttgaaaa
    20521 ataaataaat taatataaac taaaataaag cctacaaaaa agaggtgata aatagaaatg
    20581 gctaaaatac tagttgtaga agatgaaaaa aggatgcaaa gtattattgt agaatatatg
    20641 caaaagggag gatatacttg tgtaactgct gatgatggag tcgaggcact tacaatatta
    20701 aagagcaaca atatagattt aatgatattg gatgttatga tgccgtattt agatggattt
    20761 tcagtgtgta gagtttcacg tgaaatgact aatataccca ttattatatt gacagctaaa
    20821 ggtgaagagg aggacaaatt aaagggttat gaatatggtg cagatgatta tataacaaaa
    20881 ccatttagcc caaaagtact tttagcaaaa gttaatgcct tgttacgtag atatactact
    20941 ggcattccaa aaaattcatt gattttagga aagattttta ttatggtagc atcaaggcaa
    21001 gtatatgtag aagataaatt gattgattta acatataaag aatttgaatt attgcgttta
    21061 tttatggaga atccaaatca agttttttcc agagagaaac ttttaaattg tatatgggga
    21121 tatgattttg aaggaaacac acgaactgta gatacacata taaagacctt acgcaaaaag
    21181 cttggtagtg aagggcatca tatagttaca ttaattcgtt caggatataa gtttgaggtt
    21241 aaggaatgag tagtttaaga aagattatag aaaaattgaa aaatttagat atttcaaatt
    21301 ggattaaaca aaattttgga ctacattcag ttagaaaaaa agtgttttta ctttctaaat
    21361 tatcaggggc gttaatcata ctattttatc tggttgttga agaactttcg ataaactcta
    21421 aatttgaatt ttggatttgg tttataatac tggttatatt tattatgagt attgattttt
    21481 tattgggaaa atttatttca gaaccaatca catccataaa taaatctgca aaaagcatgt
    21541 ctcaacttga tttttctaat ccatgtactg taaatactaa tgatgaattt ggagaacttt
    21601 ctaggagctt gaatacaatg tctacaaatc tacaacaggc attatcagac ctagaaagtg
    21661 caaatattca acttgaaaag gatgtaaata aggaaagaat gctattggaa caaagaaagg
    21721 agttggtaga tacaatttca catgaaatga agactccact tggcattata cgtgcgtata
    21781 cagaggggct tatggatgaa gctgatgaag aaaaaaagaa aaattatatg aatgttataa
    21841 tagaagaaac tgatagaatg aacaatatga ttgtatcatt attagactta tctgcattgg
    21901 aagcaggagt ttctaaactt aacccagagc gttttgattt tatagagttt gttgaaactg
    21961 tggcaggtcg tcttttaatt gatataccag atgccaactt ttattttaca tatgatttgc
    22021 cagagtatga agtttttgta gtggtagata aaatgagaat ggaacaagta gtagagaatc
    22081 taattattaa tgcaaagaag catgttgtat ataatggtaa tttagattta tcgattacat
    22141 gtgaaaatgg cttgatttta tttgaaattt ataatgatgg taggattata gagcaagatg
    22201 agatttcaaa gatttggtct aagttttatc gtagtgttca atctcagcgt acaggaggtt
    22261 caggtctagg gcttgcaatt gtttcacaaa tacttacaat gcaagggtta gaatatggag
    22321 tgaagaatcg tgatagagga gtagagtttt attttatgat tccaattgat gaataattta
    22381 gtattaattt ataaattata agtatttgat gtaaaatatt tttctttgta gacaatagat
    22441 atctaaagag aataaaataa aaaaatttaa tgaaaaaagc ttaatgaaaa agtctaataa
    22501 aaaaatttaa taaaaaaatt taataaaaag cttaatgaga agtcttgttt tcctgatgaa
    22561 aacaagattt tttattaagc tttttattat ttcaccttga tttcacaaga gagacacttt
    22621 gatttcacca aatagaaata aaataagtta aataaattaa aaaatagaaa cgtaattaaa
    22681 taagcttaca agggggagta cttatgaaat ggtatacatg gttgactatt atattgatag
    22741 tattggtttc tattccgctt aaaattaaat ttataaaatg gtgggacaga cgacaaaaaa
    22801 aatgataaag gaggttatga tagatgataa aagtagaaga gttgaaattt tcttatggaa
    22861 aagataaaca gatattacat ggacttaatt ttgaagtaaa agaaggagaa atatttggat
    22921 ttcttggacc taatggttca ggaaaatcca caacacaaaa aatcttaaat ggtgttttaa
    22981 aaggttacaa tggtcacgta tccatttttg gtaatgaagt taaagcatac acagaatctt
    23041 tatatcaaaa gattggtgtt ctatttgaat ttccttattt atataccaat ttaagtgcaa
    23101 ttgataattt agaatatttt tcgtcttttt atcctaaaaa tcaaaggaga gacatatatg
    23161 aactcttaga tttgttagag tttaagaaag agtttataaa taagcctgta tcatcctatt
    23221 caaaaggtat gaagcaacgt attagcatgg caagagcatt ggtaagtaat ccaaagttgt
    23281 tgtttttaga tgagcctact agtggacttg acccatctgg agctgtacta tttcgtaaga
    23341 ttattgaaga agaacgaaaa aaaggaacaa ctatcttttt gactactcat aatatgctag
    23401 atgcagattt aatgtgtaac aatgtggctt ttattgcaga tggcaagatt atggcaatag
    23461 ataaacccaa aaatttaaaa atgaaaaata gcaataataa agttgaagtg gaatttgtat
    23521 ataatggaag tcgagatatt aaaattgtag atataggaga attagaatct ggaataactt
    23581 ttaaatatga cgaaatctta agtgtacatt caaaagaagc tacactagaa gaagttttta
    23641 ttaaatatac aggaaggatg ttagtgtaat gtattctaaa ttttttacct tagtaaaaaa
    23701 ggatttttgg atgatgattt atggtaaatt ttttgtacta acacttggct ctcttatact
    23761 ttattcatgt tatattaatt ttggttatgt aaagtttatg gatgaagata gttatcacaa
    23821 taatgtatat ctatataatc cagagaatat aagcatgagt acatctccat taattaatca
    23881 agtttcttct ataggtgaat taaaagcaaa acttgatgaa gatacaagtg cagtttgtat
    23941 tgatatgagc tccagtaagc cgaaagtaat tttacatgag agtattaaag gcgttgacaa
    24001 ttatagagct gactatgcat tgtcattatt atcatcagaa aaagaccata atagcaaaat
    24061 atcggacaac aatgcaataa ttcaaattaa tgataaggat acatctgaga taaaacaaag
    24121 aaaagaaatg acttgtgaga ttttattctt tgaaattgta gctattggat ttttagggat
    24181 tgcctcttta ttatttaaag aaaagcagat gggggtaatg cgtattactg gtattttacc
    24241 tgtaaaaaga agccttttta tattatcaaa gatatttgct tttttgatat gtgacctatg
    24301 ttttacagtg cttttagtag tatttaatgt tggatttttt gaaggaataa aaatactttc
    24361 acaagtatta gttcaagcta gcattttatc aattattatg tctttgattg gatttggatg
    24421 ttcaatgtta ttgagagatt ttaagcaatt ttctcttata tatcttgtgg ttgctgtatt
    24481 tataactaca cctatatttc tatctgcaaa tacatctttt gaaatagatt ggattaaata
    24541 tcatcctttc taccatgttt atatccagct taaaaatgcg tttttcatga atttttcaaa
    24601 tgaattaatc tattttgtag tgtgttcctt gacaataata gttctatttt tacttgtaaa
    24661 aaatattttt aataaagagt tggttaggga gggataatct tgataggatt acgttatcag
    24721 ttgaagagta taagaaaaga taaaatgtgt ataatttcat tctttttacc agttgtcatg
    24781 gcgattttaa taaattttat gggaacaatt gatatgtctt cattaggaga gatacatttt
    24841 ggtatagtaa aggatacact tcctgtacga acagagcaat ggttgaagaa gtatggtgtg
    24901 ttaagtgtat atgacacaaa aatgcatcta atacgtgaaa ttaaggatat taaaacggat
    24961 acaattggag ttgttagaga tggtaaaggg ataaaaactg tgctttatgg aaatgagatt
    25021 aatgctataa aaaatgttgg aaagacattg ccagagatgt ttagactaaa gggagattta
    25081 aaagatgttg aggttacaat attgcctaaa gatagtattt taaaagagtt tcaaaatgtt
    25141 tttattgcta tgattttaat tactgctatg tttatgggat gcacctttaa tgcaatgaat
    25201 attatatctg aaaaagaaga tggaatatca tttataaatg aaattttacc acagagtaga
    25261 agtcagtata ttatacaaaa agtatttttg ggtcttttat gtgggtgtct atcttcaatg
    25321 ataactgtat ttatatgttt ggatatttca atattagata tgataatgat gttactactc
    25381 gttgtatttt cagcatttat ttcatcactt ataggtttat ttattggaaa gttgtcagaa
    25441 ggtcttatgg taggaattgt ttatattaaa atagtaatga ttttatttat aggtgttcct
    25501 ttgttaatat atcttctagg ggttaataca agcggtatag ggaatttatg ctatataatg
    25561 ccgtcagttg ctacatttaa tggtattatg gagattatta atggtaatgt cattattata
    25621 aaagatattg taattttggc tgtacattgt gtagtatggt ttatgctgta tattatttta
    25681 gataaaaaaa gaaagttatc ttttagtaga taactcaata taattataat tagaacaatt
    25741 taataggtga tttaatatac tttgtatagt gtaatttatg tagtacaatt tgtatacttt
    25801 aatttatgta gtgtaatagt tagttttatt ttataattat tcaatataat ttagtaagaa
    25861 gttcagtaca attagagcta cttagtggaa aatttaatag gtacttaaac tatataaata
    25921 ctttattttc ctccatttgt actataaatt taattgatga tataaatgga ggtattatta
    25981 tatgatttga taaatttcat cattataact tacaagtaaa tgtattatta tatgagatat
    26041 tattatataa agttaataaa gatagtatca aaatattatt tgttataaag agaggggctc
    26101 aaaataaaga taaacctatt tttcaatgtt gaaatgataa aaacatattt gattttatat
    26161 aaaaaatttt cttcatgaat taaaaagtcc attttgagac ccctcatttt taaccagata
    26221 aatcgagtta aactatttaa atatattact cggattcatt tgacttagat attccttgct
    26281 tgtaaaattg ttttgagtag ggaatagcca aagcaaaaaa taaaagtata atccctgcta
    26341 cgattattaa attattaata gacattacat ctgccattgg gccgaaaatc atcataccaa
    26401 gaggaataaa accagtatat ataataatag gtaagctaag aactcgacct agcatttgtg
    26461 gtgctacatg ctcttgcaac atagtcatag ctgatgcttg aatgattgga attgaaaaat
    26521 tcgtaaaaaa cataaatata aaaaattgcc acgcttgtgt tacaagacct agaataattg
    26581 aaaaaatagc ataagataat attgctagaa aaaaggtctt atttctattt ttaaaaccac
    26641 caaaaactcc tataatcaat ccacctaaaa ccatacctaa aaatccaatc atttcaataa
    26701 tagacaggta tatataaccc ttgccaaatg ttcgttcaat aagaagtcct ataagaaatc
    26761 ctgatggaac acaaagaagc atgaatgctc cataagtgaa aagcactttt ctaatgaact
    26821 tatctgaaaa acagtacttt atccctgata ccatatctgc aaaaaatgag actttttcag
    26881 tttgttttgg tatatcatgt ttaggaattt tgataaatat caataataca atagcaataa
    26941 ttgcagtaaa tacatcaatt aaaagaactt gatttatttt attaccaaat ccaaggattg
    27001 cagcagccac aataggaact ataaattgta ctatagactg taggctacca tttataccat
    27061 taaaacgcat taaatgttct tctggtacaa gttgaggaat cattgcgttt acagcaggtg
    27121 tttgtacacc acttcctagt gaacgtataa cagatattac tgatatggct attaattcac
    27181 ttgttgttcc atttgatgac atcataaata gaattagaac taatgtagat atagcaattg
    27241 ttgaatctga tacaattatg agtacctttc gattgtagta gtctgcccat acaccagcaa
    27301 aaaaagaaat tatcatttga ggtataaagg agcatatagt taaaactgtt acccatacac
    27361 ctgatgatgt ctgaagtgtg acataccata tcattgccat ctgtacaagt gatgagccaa
    27421 ataaggaaat atattgactt gctagaaaaa gaatgactct tttcttccat gtagaattaa
    27481 tatttttgtt ttccatccta ttcctccttt gatttgtgtg tttaattttt ctcgctctta
    27541 aggataacac acaaatcaaa gtctggagtt agagtggagg gctaggaaaa ttgttttttc
    27601 ataatttgaa gatgagattt cattgttttt gcattatgtt cagcttcttt caatgaagtt
    27661 atcagtttgt cacacactga gataatttcg tcatcatctt ttggagtgtc aaggacagtt
    27721 ttaatattgg tattaggatt agaagataaa gcattgagca ttcgtaaaat agctgaaaga
    27781 gaatagtttg cacaacgaag tgcacttata attttaagtc gttttatatc ctcatcagta
    27841 tatacacgat aaccattttg ttttcttttt acttttaaca aaccattcat ttcccaattt
    27901 cttaaagtat ctattgtaac atttaagtgt tctgcagttt cttttctagt taaaaagagt
    27961 ttatctgttt ctggagtatg tccatttaaa atctgtgtaa caatagtaag tgcttcctcg
    28021 gcattagatt tttctatttc taattgtttc aaatattgat ttacaagaca aatagccttg
    28081 tcaaaatctc tatttgctga tgttcttaca atctcaaggg catcttttct taatcctctt
    28141 tgtaccactt caattttcaa ggctgtacga gcaaatctaa attgttctat atgaaaatca
    28201 ttaaagatac gataaccatt ggtattacgc ttaggtttag gtattaagct tagctcttca
    28261 tataaacgta ctgtattagg atgtacacca attaaatctg ctacctctga tgtcgtgtaa
    28321 gtgtccatta gtattcaact ccttttagat atatagtatt agtcataata acatcaaaca
    28381 aaagtctggt gttatagtgg agggtttggt tatctaagag taattagtct atttaagatt
    28441 aattactcta ttatagaagt aagtatatac ttttcaataa actcccatct atcacgaggt
    28501 ttaaacacta ttttagatgg tatggctatt actaagtcat attgtggaat acaacaaata
    28561 gcatttcctc cagctcccat tgctgaatac cacaaaactc catctttttt acatagccac
    28621 cacagataac cgtattcatt actattcatt ttaactgatt cttcaatcca cgatttagat
    28681 ataatacgtt tattattcca aataccatca tttaaataca gaaaaccaaa tcgcaacata
    28741 tctctagcac tcaatgtaag tccccagcca cctgtagaat agccctgtct atcttttgcc
    28801 caacccttaa gattttttcc aaagaaatct tcaaaaccaa aatctttcat atcataatct
    28861 ggtataattt tcataccaat aggtttaaat aagtattcat tagcaaattc acgagcagat
    28921 tttcccgtag tttttgttat aatagctgaa agtaggtgtg ttccagctga agaatatttg
    28981 aaagtaccaa tttcaccttt ttgaccaagc tgattcaagg tatattttat ccaatctttt
    29041 tgtttgcaaa gtctctctaa agactcagtc atatttttaa aagggtatgg tgcagtcatt
    29101 gtaagtaggt gacgtatagt tactgctttt ttacatattt cagaaggact acaagtatat
    29161 tcagggaaaa aatcaacaac cttttggtca acatctttta tatatccttt atctattgca
    29221 atgcctatta aagctgatat aatacttttt gtaactgatg caacatgaaa agtatcgtca
    29281 gcattatatc cattgtagta tttttcaaag acaacttttc cttttcttgc aataattatt
    29341 ccatttatat tgctatattg tgagattatt aaaggttcaa gttgtgaaag cttttctgaa
    29401 ctcataccaa cacttgaagg gtctactttt tcaaaaatta agtctaagca attgttttta
    29461 ttcattaaag ataccttcct gttatagatt ttggattttc ttttagagtt tttggagaac
    29521 cttctgcaat tatataacct cctttgtcgc caccttctgg accaagttca acaatccagt
    29581 cacaaacatt taatacatct atatcatgtt caacaacaat tacagaattt ccatttgcta
    29641 ctagttcatc caacaattga atcagcttag cagtatcata tagacttaga ccagtagttg
    29701 gttcatctaa tacataaaga atattaccat ttcgtttttt accaatttct tttgcaagct
    29761 tgactctttg ggattcccca ccacttaaag ttggtgtagg ttgacctaat tttatatatc
    29821 ccatacctat ttgttctaac acatttaagt gtgaaacaat atttggaata tctttgaaaa
    29881 aatttacagc ctcagagata ctcatattta aaatatcatg aatagtttta tttttatatt
    29941 taatagataa ggattcatca ttaaatcttt ttccatgaca gtcactgcat atatggtcta
    30001 tacttagatt atttcctaac cagacttttt catatccgct accactacat ttaggacaag
    30061 cccctttgga gttaaatgag aaatgaccag ctgtaaatcc tcgctctaac gattctggct
    30121 gacctgcaaa tagtgtacgt atcttatccc atataccaat ataagaagca ggagttgagt
    30181 tcatattttt tccaatagga gattgagata tttctgaata tccagaaata tacttagtac
    30241 caatcaatct atctgcaata gtttcgacaa ctgcataatc atcttcatca cctatttcac
    30301 tctctgaaga atttgtttga ttattgatag tatggttatt aaattgactt ctaagaagag
    30361 gaagtaaggt gtctgatata agtgaactct ttccactacc tgatttgcca gatattccaa
    30421 ccatagcacc caaaggtaat gatactgtta catttttaag gttatttgta tttgcatttt
    30481 gaacagttat acagggtatt tcatcaatat gtgtaatatt tttagtgctt gttctattag
    30541 gcattgaata ttttccagat agatattgtc cagtaagtga tttattggat tgaagtagac
    30601 cttccaaatt tccttgatat actaattgcc caccttcaat accagctttt ggcccaatat
    30661 caataatatg ttctgccatc tcaattgtgt ttttatcatg ctcaactact ataactgtat
    30721 tgcctaaatc tttaagtttc tttatggatt ttaagatgtc tattttttct gactcatgca
    30781 atccagcagt tggttcatca agcacataaa ttaatgaatc catctctgaa tcaaggtgtg
    30841 aatttaaaaa tagtctttgc atttccccac cactcaaagt tgatatttct ctgtataagg
    30901 atagatgacc taatcgtgat tctattagat gtcgagtttt ggacaaaata ttatttacaa
    30961 ggtttgtacc aaatgtgctt aaattacctt ggtctaataa gattttaagg aactcatcaa
    31021 tttctagaat cgtcattttt ccaagttctc caatatgttt tccgtttaaa agtactctac
    31081 gtgcttcttc accaatttta aaaccattgc aatctgaaca ggcagtcata gtataaactc
    31141 catttaaatc ttcgcctctt ttgtaacgat tgtgaaatac ctttgataaa gaggaacttt
    31201 ttttgccatt ttcataatga ccatagagca cttcattttt tacttcttca ggtaattgta
    31261 agaattgtat gtctaaataa tcgttgaatt ttttagagaa tactctcaaa tatcctggtg
    31321 taatctttgc tctatctaag acttgtttta gggtatcatg cttatcaagg actaattttt
    31381 caatattaat ctcaaaatat gcaccatgcc cagaacattt catacacatt ccatctgaag
    31441 agttgtatga gaaataactt ggctttaatc gttcttcttc atacccacaa ttattgcaaa
    31501 ttaaattatc gcttacagga gtatcacaca tagtacacat tatttgacct tctactgaat
    31561 acaatagtgt aagcatgttg agaatattgg ttcttgtgcc tactgttgaa cgaggattac
    31621 tttggcgaat aatattttgt tgaacagcta tagtgggagc taaaccttga atattatcga
    31681 acttatcctc actatcaata cctgcaagta tacctaaaga ttgaaggtat tgtttacgtc
    31741 cttcctcaaa tacaatgtca aacataaggc tagatttacc tgagccactg acacctgttg
    31801 caacaaccag cttatctttg ggaatagaaa catctatatt tttaagattg tgtatctttg
    31861 ctccattaat ttttatttct ttcattggtt cacctcattt tatcaatttt atcaaaccat
    31921 aaaataggga tatattaatc cttaatacaa gaatagatta ggtgaggtta ttaaaaaggt
    31981 tctataggta aatatatatc gaccaagtgt ctattttgag ggtgattgtg tgggttgtta
    32041 agatatactt caaaagggaa ggaagcacgt agcttataat taacatttga taaccattca
    32101 ttatacataa aatcccacgc atcactatat tcagattttg aaatatcaaa atgtcctaca
    32161 agatattttc cagaaggtat tatcatattg ccaagattgt cattctcttt gacaacaatg
    32221 ttatctggaa tggtcatgca aatactagtc cttaattgat gttcctgtgt aaattgtgga
    32281 ttatcatgat aaattgctaa tatttgtgtg ttttcaagtc caagtagatt ctgctcactt
    32341 gcatatgtga ataacctttc taaaagtttt gggaaagtac tagttagatt accatatgaa
    32401 ccagtatatc tagtgtaaat agtattcata ttatcaagtt ctagaatttc aatttcacct
    32461 ttaacctctc tagtattctt acatttaggt tttgatatgc ttctattgta ctgagaaatt
    32521 ttatatggtt ctttgcaatt cttgctataa ttatttctat attttatagg acttatttta
    32581 tagtaatttt taaatgctct cgagaaaact gctgaatcag taaagccgaa gtagtgtgca
    32641 atatcagtaa tggtcatgtc agttcgatga ataagaaaag cagttgcatt ctctagtttt
    32701 agcctattta catactgtgc caaagtttct ttttctattg ctttgaagat tctatgaaaa
    32761 tgaaactttg aaaaacctgc tatattagaa agttcgtcaa ggaaaagggg ttcatgtaaa
    32821 tgttcttcta tatagtcttg cactttgcaa atacgattta gatattcttt tttactttta
    32881 gcgtttgtca taaatagccc tccttattgt gtaaaatgtg tattaattta tagtaatata
    32941 gtaataaaat tttatgtttt tgtaatttat gataaataag atagcgttca attttttata
    33001 attaataaaa gctatttata attaataaaa aatgtaagat gcattatatc tgtctttgca
    33061 tataagtata atcttcgtaa taattagtat accatagtga tttattttta attgtgcatt
    33121 tatataaata cacaacttat gatatggaag agttattatg caaataagct gtaattaaat
    33181 aaattacaat ataaactagt taaaacataa gccagaaaaa tactatcaaa tttgttactt
    33241 agcacaagag catagaggag ataaaataag attgaaaaat tacatactaa atttaataaa
    33301 atttttatat taaaaataat attaagttta ttgtttaaat aaaaaagtta aatctacact
    33361 attaacttat tatacttaaa actttaaaat gaaaatatca aagaatactt tttaataaaa
    33421 ataataattt aataagttat ttggaaatct aaaaacttta caacaaatta tctatttaaa
    33481 ttggaggagt gggaatatga gtaatagttt ttatcaatta aattcagcag tattatattt
    33541 taatgaaaaa agagatgtaa atagaataag gtttaatcac tatgtaatga gaaaagaaaa
    33601 tatacaaaga ttaaaaggaa ctctgccaag aggacaattt atgatgacag taaggaaagc
    33661 tgcatctgac cttgatgtat ctacatctac aataagtaga cttgtaaatg aatttataga
    33721 tttaggtata ttgagattaa taagtagagg agtaaaggga aattgttgtt ctgtatatag
    33781 ttatgtttgt tctgaaaata gtaatacgga gattttaaca gggaatagtg gtattttcaa
    33841 aaacatagag aaataccttg atgtaaccat taacaataat caaaaaacaa tgaaatataa
    33901 acatagagat gagttgttca agaatagtaa taccaacaca tacagccgta taaatgaaga
    33961 acatatagat aaaggttatg attcgataat tcatactggg tatgaaacag aaaacgagac
    34021 aaagaaaaaa gaattattaa aaaataattt aaataaaaag aatttaaata aaaatacctt
    34081 tgaaaattcg gatgaagatt tgaattatga aagtccagaa agtatacatg aaattattat
    34141 aagaaaatta aatagtgaat cgggaaaaaa ttttaaagct gatttaagta taactaaggc
    34201 acttatagat aaaagattaa aagaagggta tagtttagaa gatttttaca aggttataga
    34261 ggcaaaggta agaagatgga gatatacaga tatggaaata tatatagaac caacaattct
    34321 gtttgggtat gaatttgagt cgtatttaag tgaggagagt tctttatctg cttgtaatta
    34381 tggtgatatg tgttatgagg atatgtattg agatatttat taagatatta gttgcttttt
    34441 agttgtttaa aatatgctta tatataagta taactagtgt attaaaaatg gaattgtctc
    34501 aaaataggga gaagcctctt tttaattgtt taaatgatga aaatatattt gatttaatac
    34561 aaaggagagt gcctcatgag ctagaaagct cattttgaga cacccacttt ttttaatata
    34621 ctggagaaat ataacccatt tgaatggctt ttgtaatagc ctcaactgat gatgaaacat
    34681 ctagcttgtc atatatgtgt tgtaaatgat ttgataatgt tctttcactt atatacaatt
    34741 catcagcaat tatttttctt tttttaccac tacagataag ctgtaaaatt cttttctcca
    34801 tactagttaa atcttcaata attacttcag tattttcaga tggaaaacaa gttccccctt
    34861 gatagacttt taataatgaa tcaattaaac tatcagggct aatattctta tttaggaatc
    34921 catagatacc tattttttta gcctcatgtt tataaactgg gaggtcgtat ccagtcaaga
    34981 ttattatttt aatagttgaa ttagtttcta ataaattata agctactttt aagccatcac
    35041 attcacttaa gcctctaagg tttatatcca ttaagacaat gtctattttt ttatcttgaa
    35101 tcatttctga tagactatgg atgtcctgtg tggaataaaa ctgttcgatt tcttcgtatt
    35161 cttctagtgc aatggacaag cttttagaaa aaagcatatg gtcatcaact aacaaaatat
    35221 ttataagaaa catctccttt catagggatt gtaatctgta cacaaactcc atgaggtata
    35281 ttatcagaaa tacttatcgt tcctcctaga ttttctactt gttctttaat tgagataatc
    35341 cctttgtgtt ttattgtatc aaaagtaatt ttagttatat ctaaaggcgt tttaaaagat
    35401 gtatttttca caccattatc attaatattt aatttaatta tatttttttc tagtgctaaa
    35461 gctatccatg catggctacc atttgaatgt ttatatacgt ttgtcaataa ttctttaatt
    35521 agtcgatata tcaatatatc atatggttca actaagaata aagtatcaga acacttaaat
    35581 gaaacacaaa tgtttttttg tggaaaggat gaagaaacac tttcaattaa attctgataa
    35641 ttttctttta ctgttaaatt ttttaaaata acagggtgat agtcttgcat ttgttttctt
    35701 atacatgtat ttagattgtt aagtgtttca tatataatat cttgaacttc tgggcgatat
    35761 gacttactca tcatgttttt tattgaaagc aggttttgta atatatcatc atgtaaaaaa
    35821 ttagaaaact ctgtttttag ttgttcttct tgttgtaatt tttccaatgc aaaagtatac
    35881 ttgctatttt taactacttt atttttttca tctctaagat taaactccaa aataatgtta
    35941 cagaggaaaa taagcataaa taaaacattt atagctatta atattataga aaaattccca
    36001 ctggcaaata gtacaaccaa gctaattact aatatactta taaaaagaaa tatagacctt
    36061 tgctttatgt taaaaactgt tgaaatagga aaactatcat tttctttaag tgcaatacca
    36121 tgaatactca taaaaaatac taatacagtt agatagattc caaaattacc aatatgattt
    36181 gatattctta gtgtaaaaaa ataataagta aggaatccta taattgttat aaataccgaa
    36241 atcgatatag gttttttttc actttttatg acaaaagcga cccttttcca atgataaaaa
    36301 ataatataga aaaagcttat tatactaaca acaaactgta ttccgtacat acaagcatac
    36361 gttctgttag aaataaatgc tcctacaatt gcaccaatgc aggtgataag caataaaatg
    36421 tctgttatct tacggaactt gtatccacta ccttgaaaca aaaataaaaa aataaattta
    36481 atagagacat acgctataac tgtgcttaat attttaaaaa taatagtgcc tattggtgtg
    36541 gctacaaatg ataagagtaa ataccaactg tctagtacta gtaatccaca aaacaatgaa
    36601 ataatgttat tttctcggac attacaagag gttatatatg tgatgtctat gagtaataat
    36661 gttgaaatta ttaagggaat aatgctttgg atattcatat aagttttagt gcttgtagat
    36721 attcccatga agagaagtga tagaatgtag tttcctaaaa atagtatttt taaattaaaa
    36781 tttgtcaatc ttatacacct ccttatgagt taattatatt aattttatga ctaaatcaaa
    36841 cttaagaatt aataactttg aattatatta attttatgac tgtatcaaat ttaagaatta
    36901 gtaactttga attatatcaa ttttataaat atatcaaacc taagaatcta tagttttaaa
    36961 ttatatcaat tttatgattg aatcaaactt aggaatctat agttttaaat tatatcaatt
    37021 ttataaatat atcaaactta agaatttata attttaaatt atatcaaata cttataaatg
    37081 aagtatacca aatcgtcaca cctttaagct tgacgatttg gtaaatatta tttttttagt
    37141 acaattttac aacaggattt aatagtaaat atataatata tcaaatacac tacagaagtt
    37201 aagaaaattg caataatcac aggtatgtat tgtgaaagtg aatttccaag taaattctgc
    37261 atcaaagcag ttttataaac caaggtagca aatatacaat ccactatacc aagtaaaaac
    37321 ggaatattaa aaaatggcaa tacttgtttt ttaataattt ttttaatttg tatattagta
    37381 taacccattt tctgtaaaat tttataatca gatgttgaat ctgaaattgc tgatatattg
    37441 ttaaagtata aaatacttcc agaagcaata aagaataata ctacaagaaa tccgattaat
    37501 agaaatgtag aactattaat tgataataaa tcatttatcc tgtgagaata tccttgtaaa
    37561 taagggcttc catctaaata atctgaaata ttactatata aattttcatt atttttaatt
    37621 gatttaccat taatacttaa aatgcttgtc attgggaatt tactttgtgc tatttgctca
    37681 tatatactat cattaacgat aagtgttcca acgctattag caaaagaaat agcattgttt
    37741 aatgatgttt cccttacagt tacaggtgtt tctttaccat taataagtaa tagataaatc
    37801 gttccatttt cactatatcc atcattgttt ggttgatatt tcattaaaat acattcatta
    37861 tcccttaaag gggtaagttg ttttattagc tttgttcgat tttgtgcctt taacaaagta
    37921 taataatcag aaaaagatat acattcaaat cctgattctc ttgaaataac ttctttagaa
    37981 gaactactat tagataaatt attcttagat gcaccaattt catattcaaa tggaaggtct
    38041 tttgatgaag attttacctt ataaatattt gtctgtgtaa aaataatatc gtctgtatta
    38101 gacatgtatt gactgacaat ttgttttaca tcttctattt gtttattttt ttctactcta
    38161 aattctatct ctgaaggtgc aatacgtgaa acggatgcaa tagggtagta taaagacagt
    38221 gccatgacac ttgaaactgt caaagtagca gcagagagta atgtaagcat aattagagtt
    38281 ttagagtttg aacggatacg atagataaag tttggaatag ttattatctt tgtacctgta
    38341 tagaaagctc gtttattttt tttactcttt tgcataatat atggaagaaa ggaattgata
    38401 aagagaactg taccaattac aatgataatc atagttatca agcctatttg ataaaatcca
    38461 atacttatcc atactgactt agctccacga agaatatcta gtgataaaca atacccactt
    38521 aaaatcataa taaaccccat aaaggctgga atttttctga acttcaattt tttttctgtg
    38581 cttttttcat atcgtatcaa ttttataagt gaagtcttat aaagaaattt accatttgat
    38641 atagctaaga ctaaaacaac taatgctata aagataattg ttttgtttat tgcatttata
    38701 ttaaaaaatg gtatctgtga attatcaatt gataaattaa gtagtgctgt aattccaaag
    38761 acaatactct tatgtaccaa tgctcctaat accaatccaa taataaaagc tccactacaa
    38821 gttaatatat tttcaaaggt aagtagcgat aagattgtaa acttacgata tccaagtaaa
    38881 gcatatatgc ctaattcctt tgtacgacgc cttaagaaga atcgatttga gtatgccata
    38941 tagaagatga caaatgccat caaaaatatc gaaataacat tacacataga ttcaactcta
    39001 ccatcagttg aaattttttc tagcattacc ttattaacag aaaatgaagt aaatgcgaaa
    39061 aatacagtaa taacaaaaga gactgaaagt agatatagag aatagaatga aaagtttttt
    39121 ttaagattct ttattgccat ataaaaaatg ttcattaaat tatcctccta tcttttagta
    39181 tccaattttg caatttcttg aataatcaac tcataaaatg tatgtctatt attctgttgt
    39241 ttatttaatt ctttaatgat atgaccatct ttaaaaatta aaattctatt tgcaaaactt
    39301 gctgcatagg catcatgtgt taccattaaa attgtcgttt tcaactcttt atttgcttct
    39361 tgtagtgagt ggagtagttc agtagctgaa ttagaatcca aagctcctgt tggttcatct
    39421 gcaaagagaa tactaggctg ttttactatt gctctaactg ctgaagttct ctgtctttgc
    39481 ccaccagata attcatttgg gtatttgttt aattgagaag ctatgccaaa tcgttcagct
    39541 agactcttta ccattttttc tattttatct ggacttagtt tttgaagtgt aagaggtact
    39601 gaaatatttt cgaaagttgt aagactatct agtaaaaaat actcttgaaa gataaagcct
    39661 aaattatcct tacgaaaatc agcagcatta gtgtcattaa gcgatttcaa atttatatta
    39721 tttatgaaaa tgtctccact tgttggttta tcaattgtag ataatacatt aagaagagtt
    39781 gttttcccag aaccagaagc tcccattata cctataaact caccttctga tacattaaag
    39841 gataccttat ctaaactcgg ctgttgtcct ttataagttt tagttatgct atttactttt
    39901 aatattgtat tcattgtata tgcccctttc atcttgatat agtcaaatta tatattaacc
    39961 ttattattca atcatgagta aatcacgcaa taattctaca caaattactt atatcataat
    40021 atattgttta aaatctaact ttattaggtt tgggatgatg gacaagattt taatttaaaa
    40081 gagtgcatag atatataatg gcataatgtg gatttaaatt aaaaaaataa agccataaat
    40141 attgaaaaat tcaatattta tggctttatt gctttgtaat aattaataga atatatattt
    40201 tttattaatc atctatttca aattctaata ttttaccagt aattgcatta attgttatct
    40261 catattcagt ttttcctttt attatttcac ctttatattc tttgtcatct tcatcatatt
    40321 taaattttac tattttccca ccaggcactt tatctagtat tattgattta gctttttcag
    40381 cactaataat agaactgttg tcattagtat tagagttatt attgtgatta ttatcattat
    40441 catcaatgtc atcatcagaa tcattgtcca tatcaatatc tataattctt ccatcaaatg
    40501 cacttatctc taattcatat gttttcttat tttttattac tttagcttca tattcattat
    40561 cttccgcatc atatttacaa tagattaaat taccattagg aacagcatct agtgctatag
    40621 ccttagcttt atccaatgat atattttttg tacctaaatt attatcattt aagtcatcat
    40681 ctttttcaat ttctattatt ttaccagagt aggcatcaat ttctatctca tatagaacat
    40741 tatcctttac aactttgcag tcatattcat tatcatcttc atcatagtaa caaactaaaa
    40801 tatcacttcc tggaagagtt tgttttaata tttctttagc ttgtattaaa gatatttttt
    40861 tagttttatt tcttatgttc acattcttta gttttaaaat atcttcaaac gctttttgat
    40921 tatatcctcc tcctacttgt gttatttcat ctatatcttt attttcaata aattcctttt
    40981 gactatttac aaggctacca ttagatatca ttataggaga attttcttta gcagctagta
    41041 ctcctattga taatgaatct actagtgcat tttcttggct aataccatct ttagctacaa
    41101 agacattatt tattttttta tctttataaa atttatctat aatttttgca ttagtattgt
    41161 ttctatctga accactaatt ctttctctgt ttggatattt ctttactgca ttatcagata
    41221 aaatcttatt tcctccaact atgtaagatt tttttataga cctactgttt aagaaattta
    41281 cgctactatc attgctgttt tcatctgata ctaatattat aggcatatta tgtattgctg
    41341 ctggagaagc tatactaact gcatcagata atccctttag accatttaca actgcaacat
    41401 tttctacctt ttttatctta tctatttctt taagtacatt tgtagacatt tcatagatat
    41461 tgtttcctac tattttattt atttttatgt taagcgcctt tatttcattt tctactttag
    41521 aagaaatact atttccacta tctacaatgt acacattgtc tacaccaagc cttttaagtt
    41581 cagctttagt tgaactttct atgctattgt tgtttactaa aagtataggt gcattcttta
    41641 gcttagcaaa tggatttgca caaagagcac tttgaataga gttaccatta actataatgg
    41701 ctgtttttga ttgaacccat cctaacttac ttacttctac agctgtctca tatcttgttt
    41761 tacctattaa tctttcttgc tttgtagtat tgtctgcaaa aatcgatgaa gagctagaaa
    41821 aaaacataat acccgatatt aatatagcta aatttttctt attcatattt ttacctcaca
    41881 atctttaaaa attttttatt tagcatactt aataaaatat aattttaaaa tcaaattgat
    41941 actgaatttg attttagaaa ataaacaaat aaaatttctt tgatacaaaa ttttcctttt
    42001 atatttaaca ttgtatcaca aatataaaga ggaaaaaact ttttaaaaag agtatacact
    42061 attcattttt atcatcttat aaataaatct ttaccactgt tttatatgtt ttaaaataga
    42121 cacaaaactt atttctaaat ttactacaaa tattttaggt gaaatttctt tttaagcata
    42181 tctaaaaaat atttgtagta atgctaaggg attggatttc ttaaagtata tatttctttg
    42241 gatgtttgag tttgtgaaat ttgagaattg gagggtgaaa ggaacactca atggattaat
    42301 attaaaaact acatttgtac aataattgcc ttaaaactaa aaaaatctat tgacttgaag
    42361 ttaactccaa gtagtaaagt ataattatac aggaattata tacttgtaag aatacttaaa
    42421 gtagagagat aaaacaaata agttttaaag ggggaagtaa aacaatggag acaaaaaaat
    42481 taggatttgg attaatgcgt ttaccaataa aagatgaaaa tgatgttaca actattgata
    42541 tggaacattt gaataaaatg gtagacactt ttttagaaag gggatttaca tattttgata
    42601 cagcttatgt gtatcacatg ggaaaaagtg agatagcact tagagaatct ctagtaaaga
    42661 gacacaaaag agagagtttt actgtagcta caaaacttcc attaatggct ttaaagaaaa
    42721 aagaagagca agaaaccatt tttaatgagc aattagaaaa atgtggtgta gactattttg
    42781 attactatct tttacacaat ataggtgtgt cacattatga agtagccaaa aaatttgatt
    42841 aaaaaatagc tacgcacact cgtgacttta gtcatgagtt agtagctaaa ttagtcagca
    42901 tatagggaaa cctatatgta gagataggat agaacctatc caatactact tgaattgcta
//
LOCUS       DAFNNB010000015        41294 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0015, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000015 DAFNNB010000000
VERSION     DAFNNB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0015"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(403..1470)
                     /gene="rsgA"
                     /locus_tag="L3M60_000733"
     CDS             complement(403..1470)
                     /gene="rsgA"
                     /locus_tag="L3M60_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBN6225429.1"
                     /translation="MNGMKEIDLQDYGLNSNFITQSMKYHDLYIARIISQEKNLYQAV
                     CTGGIVFAEVSGKLRFEATIPSDFPTVGDFVILDRCNDKNGNAIIHHVLHRKSLFVRK
                     AAGKANEEQVVAANIDIVFLCMSLNKDFNTHRLERYLSLTWESGAVPVVVLTKSDLCD
                     NVHRKLQVVNSIALGVDVVVTSAKESDGCQQISKYIKPGYTIALLGSSGVGKSSLINC
                     LLGGEFLNTKEIRIADDKGRHATTRRELFLISGGGIVIDTPGMRELGMWDVSEGVDKS
                     FADIEAYFGKCHFRNCSHTSEPGCAIWEAIAHQELSPKRWESYRRLKTEAVFADNKSE
                     YLAKKEKKFKNISKINKTIRR"
     gene            complement(1467..1868)
                     /locus_tag="L3M60_000734"
     CDS             complement(1467..1868)
                     /locus_tag="L3M60_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HBN6225430.1"
                     /translation="MNYKNRKRAYYQENSCLDNFICKNCGCIVVPEGAGTQHRNHCPH
                     CLHSLHVDIIPGDRAADCDANMEPIGVWVRKNGEWAIIHRCTRCGHLSSNRIAADDNP
                     VKLMSIALKPLAQPPFPLERITEKMEKEETE"
     regulatory      2662..2758
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2811..2987
                     /locus_tag="L3M60_000735"
     CDS             2811..2987
                     /locus_tag="L3M60_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225431.1"
                     /translation="MFRDKMDRCTHMLTAYIGSSYDYCDFIDTQLDDFILEYGENVVE
                     SCLHQVMVLVSKYN"
     gene            3397..3534
                     /locus_tag="L3M60_000736"
     CDS             3397..3534
                     /locus_tag="L3M60_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225432.1"
                     /translation="MSNQREDFDTTNDMFQESIPITTKIILDDIPSHDELNHVFSKGC
                     E"
     gene            3538..4035
                     /locus_tag="L3M60_000737"
     CDS             3538..4035
                     /locus_tag="L3M60_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225433.1"
                     /translation="MKKRKIVLITLLLIGVLLLGGILYNLFLVKTANISMLKESWNLD
                     IPIPNKEIEVFDTQDSINGDGQSYFIQEFSEKNFKKVFNLKGGIVVSKDNINEIEKYI
                     DKFKRDSVNINKSNKNKIEEDFKKYKLEVKKDDKYIYKRNYENYVVLIIKKDEQKLYS
                     LIWNQ"
     gene            complement(4512..5870)
                     /gene="rlmD"
                     /locus_tag="L3M60_000738"
     CDS             complement(4512..5870)
                     /gene="rlmD"
                     /locus_tag="L3M60_000738"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBN6225434.1"
                     /translation="MLSKNEVYIVDIVDIGQGGVGVGKYNGITVFVEGGLIQDKVKVR
                     INKVKKNYAVGDIVEIIEKSPFRVKRVCSDNLRDCGGCQIQDLDYNKQLEMKTNEVKQ
                     VLSRIGKLEDVEVHDTIGMDNPYRYRNKAQFPIQKNNGVPIIGFYKKKTHDVISTEKC
                     VIQHEINDKIVKIIKTYIRAYKVSIYDEKTHTGLIRHLVTKIGFTTKEVMVVLVANGN
                     KLPYLKELASVLKENIPGFKTLVVNVNQKDTNAILGKENIVIYGDGKINDYIGDLVFE
                     ISPLSFFQVNPVQTKVLYDKALEYADLNETDTVFDIYCGIGTISLFLAQKAKKVYGIE
                     IIDDAIKDAKINAKLNNMHNAEFYVGKAEEIVPKMYREGKTANVVVVDPPRKGCDEKV
                     LETIVSMSPERIVYVSCNPSTLARDLDYLNVHGFKCVEAQPVDMFPHSTHVETVAKII
                     KQ"
     gene            complement(6045..7805)
                     /gene="pyk"
                     /locus_tag="L3M60_000739"
     CDS             complement(6045..7805)
                     /gene="pyk"
                     /locus_tag="L3M60_000739"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBN6225435.1"
                     /translation="MLNNVKKTKIVCTLGPASQSEEVLTQLMQNGLNVCRFNFSHGSH
                     EEHKERIDMAKKVREKLNKPVAILLDTKGPEIRTGNFEDPEVFLEEGQKFTITMKDIM
                     GTKEMCTVSYKGLAEDVKSGDSILIDDGLVGLRVKEINGEDIICVVENSGIVKNHKGV
                     NVPGVKINLPAITPKDISDIEFGISQGIDYIAASFVRKASDVLAIREVLENNNATDIQ
                     IISKIENQEGVENLDEILKVSDGIMVARGDLGVEIPTEEMPIVQKMMIKKCNELAKPV
                     VTATQMLDSMIRNPRPTRAEVTDVANAIYDGTDAIMLSGETAAGKYPVEAVKMMATIA
                     KRTEETLDYDRLLKENGTNNVTVTDAISHATCTTAVDLNASAIITSTSSGYTARMVSK
                     FRPKSPIIATTNNEKTMNKLALTWGVYPVKSSVAGNTDEVIEKAIEAARQADYIDNGE
                     LVVITAGVPVGVSGTTNLIKVHVISEEIVQGIGVGTQTVEGKVRVIKNGDTCVDFNEG
                     DILVSTATDAEMNNYIEKAGAVVTENGGMTSHTAIVGINLDIPVIVSATDITNLVKDG
                     EVVTVDASRGVVYRGSTRVL"
     gene            complement(7857..8816)
                     /gene="pfkA"
                     /locus_tag="L3M60_000740"
     CDS             complement(7857..8816)
                     /gene="pfkA"
                     /locus_tag="L3M60_000740"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBN6225436.1"
                     /translation="MKTIGLLTSGGDAPGMNAAIRAVVRSAIYYGCKVYGINRGYKGL
                     LEEDLTEMNLSSVGDIIHRGGTILKSSRCEEFKTEEGRLKAVKILKKYKIDCLVVIGG
                     DGSFVGAQKLSDLGFPAIGIPGTIDNDLAYTDYTIGFDTAMNTIIDAIGKIRDTSSSH
                     ERVNIVEVMGRHCGDLALYAGLAGGAETIIVPEVEITVDEVALRLKTTQKRGKRHSII
                     VLAEGVGSASDLEKELKKESGADLRVTVLGHVQRGGSPTVSDRILASRLGVRAVELLL
                     DGKSARVVGIKENKIIDLEISEALAQKKVFDKEAYEMAKILSI"
     gene            complement(9025..12594)
                     /locus_tag="L3M60_000741"
     CDS             complement(9025..12594)
                     /locus_tag="L3M60_000741"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBN6225437.1"
                     /translation="MNKDFAHLHVHTEYSLLDGFSRVKKLIKRAKELNMSSIAITDHG
                     CMFGVIDFYKTAIKEGIKPIIGCEVYTAARGLRDKDPNYDKYQGHLVLLAKNMEGYKN
                     LIKIVSTSYVEGFYYKPRVDMEELKKHNKGIIALSACLAGDVARALMNRNYEKAKQFA
                     FDYRDIFGEENFFLEIQDHNLPEQKEVNSGLVKLSKETGIPLVATNDVHYVNKEDSKI
                     HDVLMCIQMGKTLNDPNRMRFGSDEFYLKSREEMEELFPYALEAIDNTVKIADMCNVE
                     FDFNTIHLPKYDVPEGYTPETYLRELCFNGLKERYDNPSDEILERLNYELDVIEKMGY
                     VEYFLIVWDFINFSKENNIIVGPGRGSAAGSIVAYTLKITDIDPIKYSLLFERFLNPE
                     RISMPDIDIDFCYERREEVIDYVKRKYGDDHVAQIITFGTMGAKAAIRDVGRVLDIGY
                     NKVDKIAKEIPFALGMTIDKALDTNPNLKELYDQDPETKEIINISKQIEGMLRHASTH
                     AAGVVISKNPVDEYVPLYKHQDAITTQFTMTTLEELGLLKMDFLGLRTLTVIRDTLDL
                     IEKNRVIKNYTEKIDFSKMDYDDPKVYEMLSSGNTLGVFQLESAGMRSFMKQLKPDNF
                     EDIVAGISLFRPGPMDSIPAYIKNKSNPKDVTYLHEKLKPIMEVTYGCLVYQEQVMQV
                     VRDLGGYSYGRSDLVRRAMSKKKMDVMEEERQYFIHGKFDEDGNIEIAGCIRNGVEEE
                     IANKIFDDMIDFARYAFNKSHAAAYGVLAYETAYLKTYYPVEFMAALITSVMGNTDKV
                     VEYIRECKALEIDVLPPDINKSFSKFSVEGDNIRFGLAAVKNVGVNIINNIIAERESN
                     GLFVDLVDLVKRLDQKDTNKRVIESLIKCGAFDNISENRASLMAGYESLLESVSMDRK
                     KNVQGQISLFDAFGDSEEDSDFQQIYSLPKRQEYEERERLNLEKEVLGMYVSGHPLSQ
                     FEKELQEKTSMDNGKLNSLKEEEESYLQMNETDVIMGGMIVTKTIKTTKRNEIMAFLE
                     LEDLYGAIEIVVFPQLLQKYNVILNEDNIIYVKGTLSIKEGENAKLIAREIKDINDDT
                     DFVDRNYNSGRTRNVENIKPESGKKLYLKIDSMSDTDTTNNIVEIAKQYPGNDSIYLY
                     PMDENINGKRKAYKMTGALTCICDEFINNLEELLPKDNIKVKA"
     gene            complement(12739..13692)
                     /gene="whiA"
                     /locus_tag="L3M60_000742"
     CDS             complement(12739..13692)
                     /gene="whiA"
                     /locus_tag="L3M60_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HBN6225438.1"
                     /translation="MSFSTETKNELARVVSGNECCNIAELSALVKSGGSIQIVGYKKL
                     NLKITTELNSIARKVFKLLKKNFNINTTISVNKNQMLKRNNSYVLMVTSEMGSEMLLK
                     KLGILENKEGFFTINKISDNLIKHEGCKRAFIRGAFLGGGSISDPEKNYHMEFVTNNE
                     EFADSLKELINSLGFNSKIVARKNNHVVYIKESEQISDLLSIIGGHHALLSLQNTKIV
                     KEMRNNVNRIVNCETANLSKTVNAAVRQVENIKLIQETIGISSLPENLQEIAKIRIEY
                     EDMTLKELGEMLEPPIGKSGVNHRLRKIEEIATDLKKKSSQ"
     gene            complement(13709..14134)
                     /locus_tag="L3M60_000743"
     CDS             complement(13709..14134)
                     /locus_tag="L3M60_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBN6225439.1"
                     /translation="MREEVSAGGVVLFGNTILLLRKFNGDWVLPKGKVEEGENNQEAA
                     LREVSEETGVKADILKYLGEIHYTFKENWDENRAVHKTVFWYLMQAKNMDTIPQKEEG
                     FIDAKFIHLDRVVDLARYDDEKEIIKVALQEIKKRLKKN"
     gene            complement(14203..15309)
                     /locus_tag="L3M60_000744"
     CDS             complement(14203..15309)
                     /locus_tag="L3M60_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HBN6225440.1"
                     /translation="MAKLLIIVGILGWIALIISLFIYKKAKNEKVKLFQRSIVENREP
                     NVVVIGGGTGQSVFLRGLKHTTQNITAIVTVADDGGGSGVLREDLGMLPPGDIRNCLL
                     ALANIEPTMNEVMQYRFTEGLLKGQSFGNLFLAAMNGLYGNFEKAVYKLSEIFAITGR
                     VLPVTLEDVNLVAKLENGNIINGESSIPEESKIQKSSIDKIFLNPKDVKPLKDVIASI
                     YDADIIIMGPGSLYTSIIPNLLVEGIVDAIKSSVAPKVYIANIMTQPGETEGYNVLEH
                     VNAIVKHTDKNLIDYVIANNEILPEEMLDLYKQDGAEQVLLDKKQKDKLKEMGIKTVE
                     KNLIEIKNNYIRHDAKYISNIVIELALNHNYNKS"
     gene            complement(15321..16178)
                     /gene="rapZ"
                     /locus_tag="L3M60_000745"
     CDS             complement(15321..16178)
                     /gene="rapZ"
                     /locus_tag="L3M60_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBN6225441.1"
                     /translation="MKFVIVTGLSGSGKSETMRALEDMGFYCVDNLPPALITKFAELC
                     YQPNSSIDKVALGIDIRGRKFFEALHESLNYLEKENYEYEMVYLDCNDDVLLKRYKMT
                     RRNHPLAKDMQIPEGIKMERKIMEPLKELSTCIIDTTNMKPKDLKEEIKKIYSSGEDN
                     PNLTISVVSFGFKHGILADADLVFDVRFLPNPYYVEELRAKTGDDKEVRDYVMNSKIS
                     EEFYVKLLDMIHFLVPQYIEEGKQHLVIGVGCTGGRHRSVTITNLIAEDLSNKGYRVV
                     KKHRDSMLR"
     gene            complement(16209..16922)
                     /locus_tag="L3M60_000746"
     CDS             complement(16209..16922)
                     /locus_tag="L3M60_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBN6225442.1"
                     /translation="MKILADYHTHTLYSHGKGTIEDNVKVAISKGIKTIGISDHSYKH
                     IAYGVKKKDILKMREEVDYLNDKYNDINILLGMECNILDDKGNIDMDDKFIRYFDYIM
                     AGYHFGSTPTSLRSLLNHCNNYIFKTEKAKEYNTKAVANAISNNDIFIITHPGDKGDV
                     YIEEIAKTAKKTDTRLEINSSHSFLNLNQLNEIKGLENKFIIGSDAHCPERVGDFELA
                     IKTISDSNLDISLVENIII"
     gene            complement(16926..17840)
                     /gene="murB"
                     /locus_tag="L3M60_000747"
     CDS             complement(16926..17840)
                     /gene="murB"
                     /locus_tag="L3M60_000747"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBN6225443.1"
                     /translation="MNNQDIYINLLNILSKEDIKVDEPMKKHISFRVGGPADILVRPR
                     TEEQLKNVLKLVKKESIPYLIIGNGSNILIKDGGIRGVVIELADNFNSYEINDTRMTA
                     QSGALLSVLGKALQKQELKGFEFASGIPGTLGGALAMNAGAYGGEMKDIVKSVRVMDM
                     EGNIFELSNKQMEFGYRKSIISKNGYIALSAELELQEGNYDEIKSLMDDLATRRITKQ
                     PLNFASAGSTFKRPTGYFAGKLIEETGLRGLTLRGAQVSEKHCGFVVNQGEASAKDIL
                     DLIYVIKSAVYAKFGVMLEEEVKILGED"
     gene            complement(18113..18883)
                     /locus_tag="L3M60_000748"
     CDS             complement(18113..18883)
                     /locus_tag="L3M60_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBN6225444.1"
                     /translation="MSKGFLTPGETAQATISAGIKKANISTQNAILLGLFGGAFIGFG
                     ALGSMIVSQTFGKIDPGVASFLSAMVFPVGLMLVVVAGAELFTGNTLMAIALMDKKIT
                     LGKMLRNWGLVYFANLVGSILLVALVFYSSTLTGDAATKAIAVAEAKVNSTIASMFLK
                     AILCNILVVLAVWMSTASQDIMSKLASCWTVIMLFVLCGFQHSVANMFFIPMGMALGA
                     NITMVQFITNLVFVTLGNIVGGSMVVGGIYYLCYVKNS"
     gene            19202..20683
                     /gene="cls"
                     /locus_tag="L3M60_000749"
     CDS             19202..20683
                     /gene="cls"
                     /locus_tag="L3M60_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBN6225445.1"
                     /translation="MFDISFFEMSIYEIAVTTIYIINFMVILNLIFREKRNINTTLTW
                     LLILVLIPALGFILYIAFGRNISKNNMFRLKEKDDKIIKSNILDTQVKLQSTSEIDSD
                     IHQHKDMIYALANSNNAHYTNNNDVWIYAESSQFFNSLLEELKKAKKYINIQFYIFKD
                     DKIGTEIIDILIDKAKEGVEVRLLFDAVGGRTLKNSTLSRLKESGVKIGSFFPSFLKI
                     VNFNLNYRNHRKIVVIDGKVGFVGGYNVGDEYLGRNPKFGLWRDTHTKLTGDCVIDLN
                     MRFILDWRYTTKEDLDLEKYFIEEDTNSNHPSENIGIQIVSSGPDISELDEIKYGYLK
                     MIQKARKYIYIQSPYLILDSTFIDTLKIACLSGVDVRVMIPSKPDHPFVYWASYSYAG
                     ELLKFGAKIYTYGQNAFLHAKTIVIDDSICSIGTANMDIRSFELNFEVNAFMYSSKKA
                     LEQRIIFENDILNSKEITLDIYNSRSTYIKIKESISRLLSAVL"
     gene            21001..21498
                     /locus_tag="L3M60_000750"
     CDS             21001..21498
                     /locus_tag="L3M60_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HBN6225446.1"
                     /translation="MCDFISHNKQLFDELDIFIDSLATKEGALIQVLHEAQGIFGYLP
                     KEVQLHVARKLGVAPAKVYGVVTFYSYFTTEPVGKYKISVCLGTVCFVKGADKILSAF
                     EKQLGIKVGETTSDFKFSLEGLRCLGACGLAPVVTVNGKVYGKVKPDQVSEILDTYRE
                     LELNC"
     gene            21515..23401
                     /locus_tag="L3M60_000751"
     CDS             21515..23401
                     /locus_tag="L3M60_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBN6225447.1"
                     /translation="MRKVNSFDELKVLADELKPNLSLRKNYSKENVLRRELLVCCDTG
                     CTSSNSLEIVSELENEIKKSGIQDKVSVRLTGCFGFCAQGPIVKVYPDNVFYVKVEPS
                     DAEKIVQSHLIRNTVVEELLYEEESLSKKVEEQEEMSFYKKQLRIALKNCGLIDPDSI
                     EEYIANDGYLALGKCLTSLTPQEVIAEVKTSGLRGRGGAGFPTGSKWEAASKNPAGPT
                     DKKFVVCNADEGDPGAFMDRSVLEGDPHSVLEAMAICGYAIGSDTGYIYIRAEYPKSI
                     ERLKVAIAQAEKYGLLGENILGTGFNFKLELKYGAGAFVCGEGTALMHSIEGRRGEPR
                     MKTYSSSKSGLWKSPTCLNNVETFANIPAIILKGGDWFANLGTEDSSGTKVFALGGKV
                     ENVGLVEVPMGTTLRDIVFEIGGGIPEGKKFKAVQTGGPSGGCIPTEHLDTPIDFGSL
                     SSIGSMMGSGGMLVLDESDCMVDIAKFFLEFTVEESCGKCTPCRIGTTRLLEILTKIT
                     EGNGTLQDLDDLENLAETIQTASLCGLGKAAPNPVLSTLQYFKDEYIAHVVDKKCPAG
                     KCQNLLSYFITDDCKGCTKCSRVCPAGCITGSVKEQHTIDTSKCLKCGACIDNCTFNA
                     IIKK"
     gene            23424..25205
                     /locus_tag="L3M60_000752"
     CDS             23424..25205
                     /locus_tag="L3M60_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase small subunit"
                     /protein_id="HBN6225448.1"
                     /translation="MSLVNLTINGKHVSAPSGTSILDAAKLINIKIHNLCHLHMNEID
                     KLDTCASCRVCMVETERGLVPACGTVIKEGMKVQTNSAKALNARRTIVELLLSDHPQD
                     CFVCEKNGDCELQTIAADLGVRKIRYQGSKSFAGKDTSTKSLVKDHSKCILCRRCETV
                     CNDIQTVGALSGVNRGFNTLVSTFFNSDMVETECTFCGQCISVCPTGALTEVDNVPKL
                     WDVLNKKEKTIVVQVAPAVRVAIGEEFGLEPGSISTGKMVAALKALGFEHVFDTNFGA
                     DFTIMEEATEFIERIQKGENLPILTSCCPAWVNFLEHNYPDKLNLASSCKSPQGMFGS
                     IAKNYYAPKILGINPEELYVVSVMPCVAKKYEASREELSESGILDVDLSITTRELAKM
                     IKEAAIDLPNLQDQDFDNPLGKSTGAASIFGASGGVLEAALRTSYEKITNKTLDNVNF
                     TNVRGLKGIREASIDVDGTTVNVCIVNTLKNARKIMDKVRSGECKYHIIEVMACPGGC
                     VGGAGQPYHHGNTEIVDRRANALYEIDRNKAIRKSHENPDLQAIYKDFFGEPNSDVAH
                     KYLHTHYFDKSCIYGECPQECACEEAK"
     gene            complement(25345..27141)
                     /locus_tag="L3M60_000753"
     CDS             complement(25345..27141)
                     /locus_tag="L3M60_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate oxidoreductase"
                     /protein_id="HBN6225449.1"
                     /translation="MGIEYFSKLLESSTEKELLLSKKSNNISTARLISFLIVIAGFAI
                     GFYNKNIVGIFVGVVSIIIFIALLVIHSKVKEEETYFKSKSEVLNKYIKRFGDGWKEF
                     KIDGKEYLKDENSQAKDLDLFGRASLYQYICVANTSYGKNFLAKYLWNENPDENVIIE
                     RQMAIKELLSKDDFSIHIQTLSNIIGKEQKSNSDSSIESFIEYGENKNVYIPKWMHIF
                     TWGLPTAIILSFIFCVLGFLPVLPVFLLFIIQLGFSGFGYPKLMQTLSPLFSFSRSIQ
                     VYEKMFEVLEKETFESVYLKELQDKLSKGSGVSRGVKQLNSIGNAVNLRYNQIIYIIA
                     CGVLMWNYHCAEALERWKGVYGNQIRDWFESIGEFEALISLTVISHVKENTCFPVIKY
                     EDTPRLKVEEVYHPLIAEKSVIANSIQLNSQTCIITGSNMSGKTTFLRSIGVNLVLAY
                     AGAPVCAKSFDATCMAIFTSMRIQDDVSQGISTFYAEILRIKSMIQYSLKELPMLVLV
                     DEIFKGTNSADRIIGASEAVKKLSRPWVISMVTTHDFELCDLSGNGDVEVVNYHFSEY
                     YVDDKIHFDYKIKYGRCKTTNAKQLMRMAGIL"
     gene            complement(27355..28284)
                     /locus_tag="L3M60_000754"
     CDS             complement(27355..28284)
                     /locus_tag="L3M60_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HBN6225450.1"
                     /translation="METSNKVSIREIIKELKLKVVYMPDDVDYYVYSQDINRPGLQFA
                     GYFDYFAYERIQIVGKTEYNFFGLMDSHIREEVLDRFFSYEIPALIISRDLEIKPDVI
                     EKAKKYKRILLSTNRNTTRLINRLSNYLDNKLAPHTTIHGVLVDIYGIGVLIKGESSI
                     GKSETALELIQRGNRLVADDAVEIRKLDESMLVGQSPELIRHFLEIRGIGIIDVRSLY
                     GVGAIKNSKMIDLVVHLEAWDETKYYDRLGLDKEYEEILGVNVEKLVVPVKPGRNTAM
                     ILEVAAMNFRQRGMGYDAAQEFTKKLSKLIDTK"
     gene            complement(28284..30101)
                     /gene="uvrC"
                     /locus_tag="L3M60_000755"
     CDS             complement(28284..30101)
                     /gene="uvrC"
                     /locus_tag="L3M60_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBN6225451.1"
                     /translation="MFDIQEHLKKLPSEPGVYLMKDKYDHIIYVGKAISLKNRVRQYF
                     QSSKNHTSKVKSMVKNIYKFEYIITDSELEALILECNLIKRYRPKYNVVLRDDKTYPY
                     IKVTTNEDYPRILKVRRVLKDKAKYFGPYTNITAVNDTLELISSTYPIRSCKIDIDKA
                     IKNKTRPCLNLHINKCLGPCTGNVSKEEYGKMIEEIIMCLSGKEEKLMELLKEKMNES
                     SMNFRFEEAAVYRDKIKSLEEMIQKQKIDATVSDLNQDVVAMARAHNEACVQVFFIRN
                     GKIVGREHFILEGVMDSPRASILSSFVKQFYNEQEYIPKELIIEDEIEDSCILEEWLS
                     SKKGQKVTIRVPQKGEKKSLVEMVRKNAVEYLEKFSDMNKRKYEKSIGALEELKQILN
                     LEKLPIRIEAYDISNIQGVDSIGSMVVYTNAKKDKKEYRRYKIKTVIGPNDYDSMAEI
                     VDRRLKHGNLPDLILLDGGKGQVSAVKKVLELNDVDIPLWGMYKDDKHRTKGLICKEK
                     EIELDKTTNLYRFIASIQEEVHNYAITYHRSLRNKALTKSILDDIQGIGEKRKKSLLN
                     HFKDVDAIKKATMEELLEVDGMNKSIADNVYNFFRKEAN"
     gene            complement(30364..33189)
                     /gene="uvrA"
                     /locus_tag="L3M60_000756"
     CDS             complement(30364..33189)
                     /gene="uvrA"
                     /locus_tag="L3M60_000756"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBN6225452.1"
                     /translation="MEDKIIIRGAKEHNLKNIDLELPRDKFIVFTGLSGSGKSSLAFD
                     TIYAEGQRRYVESLSAYARQFLGQMEKPNVEYIEGLSPAISIDQKTTSKNPRSTVGTV
                     TEIYDYLRLLFARVGDVYCPTCGEKISQMTIQEIVDKMLDFPDRTKLQILSPIVRGQK
                     GTHKKVFDNIKKEGFVRVRVNGENYEVSDDIILNKNQKHNIEVVVDRIVIKDGIESRL
                     ADSIETAVKLSDGLVIAQIIDGEEILFSTKFACPEHGIGIEELSPRMFSFNAPFGACD
                     VCNGLGESKEVDPELVVPNKDLSIKQGAVAAWGSTGVNDDTYYSKMIKSLAEHFNVSL
                     TTPFKDLPDDFVEELLYGKDNIIVEFTYESKFGGTRNYKSYFEGVIVNLERRYRETNS
                     DYMRDKIEEYMAERPCPKCKGMRLKKEVLSVLVDGKNIMEVTNLSVNELIDFMENINL
                     TEKQRFIAHEIIKEIKGRAMFLRDVGLDYLNLSRKAGTLSGGEAQRIRLATQIGSALV
                     GVLYVLDEPSIGLHQRDNDRLIATLRHLTDLGNTLIVVEHDEDTMREADYVIDIGPGA
                     GIHGGEIVAQGTLDEIIENENSITGQYLSGKKQILLPETTREGNGSFIEIVKASENNL
                     KNIDVKFPLGKFTCITGVSGSGKSTLINDILYKGVASKVNRLKQRAGKHKEIKGIESI
                     DKVINIDQSPIGRTPRSNPATYTGVFDFIRDIFAATNEAKARGYKKGRFSFNVKGGRC
                     EACKGDGIIKIEMHFLPDVYVPCEICKGERYNRETLQVKYKDKTISDILDMNVEEAVE
                     FFENIPNIKRKLETLMDVGLSYIKLGQPSTQLSGGEAQRIKLAAELSKRPTGKTLYIL
                     DEPTTGLHMADVDKLIQVLQRLADTGNTIIVIEHNLDVIKTCDYVIDLGPEGGDKGGT
                     IVATGTPREVSKVDGSYTGQFLKKYFS"
     gene            complement(33208..35178)
                     /gene="uvrB"
                     /locus_tag="L3M60_000757"
     CDS             complement(33208..35178)
                     /gene="uvrB"
                     /locus_tag="L3M60_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HBN6225453.1"
                     /translation="MDFKIKSDFKPTGDQPEAIKSIVDSINRNEKFSTLLGVTGSGKT
                     FTMANIIQQVKKPTLIMAHNKTLAAQLYSEFKEFFPDNAVEYFVSYYDYYQPEAYVAH
                     SDTYIEKDASINDEIDKLRHSATASILERRDTIIISSVSCIYGLGDPKDYKELMLSIR
                     PGMQRDRDDVIKRLIEIQYERNDINFTRGTFRVRGDILEIFPASNDEKAIRIEFFGDE
                     VDRITEIDYVTGKIVGTRNHVVIFPASHYVTTPERIEKAIIEIEDELQEQIKFFKEND
                     RLLEAQRIEQRTKYDIEMLKEIGFCQGIENYSRHITGRSEGERPYTLMDFFPDDYLII
                     VDEAHVTIPQVRGMYAGDRSRKTSLIENGFRLPSALDNRPLNFQEFEGNINQMLFVSA
                     TPGPYEIQHSETIAEQIIRPTGLLDPIVEVRPINNQIDDLVGEITKTIEKNERVLITT
                     LTKKMSEDLTNYLKEIGIKVKYLHSDIVTLERTEIIRDLRLGKFDVLVGINLLREGLD
                     IPEVSLIAILDADKEGFLRSETALIQTIGRAARNENGRVIMYADRITDSMQNAIDETK
                     RRRDIQNLYNEEHNITPKTIQKNIRDSIEATKVAEEEVVYGISDTDDKDEIRANIDKL
                     KSEMMEAAQNLQFERAAELRDKVKQLEEKLEK"
     gene            complement(35364..36626)
                     /locus_tag="L3M60_000758"
     CDS             complement(35364..36626)
                     /locus_tag="L3M60_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HBN6225454.1"
                     /translation="MTKVIVLSVDSLFEKDLEFVKKLPNFKSILEDCSIVKNINCVYP
                     TLTYPCHTSMVTGVYPKKHGIYHNEKFDPNKENKDWYWYSKDIKVKTIIDVAKENNLT
                     TSSVLWPVMGANPNIDYNIAEIWAPSREDDPRETFERSSSKVIMDGIYNKHCHYIDWK
                     LEPNMDLFGVNCSVDIIKEYKPDLMLIHLATLDHTRHNYGLFNEEVDKALKMNDKWLG
                     DIIQATKDAGTYEDTNFIILGDHGHLRVDKVINPNVLLKSNGFIKVENGEVKNFDAYV
                     DSSGISAQIIVNNLDRLTELRELLEEFKEELFIEKIFTKEEASNLGLEGDFEMVIEGI
                     EGISFGNDLEGSIIKDSDIKDYKFAVATHGHLPTKGNRPPFIAFGPNIKSGVVIEEGD
                     LRAHAATILKIFDLKLDGVEKEAFDFIV"
     gene            complement(36689..37993)
                     /locus_tag="L3M60_000759"
     CDS             complement(36689..37993)
                     /locus_tag="L3M60_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6225455.1"
                     /translation="MKLKRKLATGLAITLLLGSLVGCSNPNDSNASKGEKTDSNITKI
                     DFWAPLGGTNGETAQKMVDQFNSEHKDVQVNMLKQKDYYENATKLQAALTSKDQPDVT
                     LLEITQTGTFASAGALVDMSKYFDKTYQKRFFSGLLTNSYYEDKFVGMPFNRSTPILY
                     INKDMATKAGLDPSGPKSWEELKDYASKMTNKSEDTYGFETPIDIWFYEAMVMQSGGE
                     ITNGKKVAFNNEAGQAPVKFWQDMMKAGIMKMPPGEDYNAWDVAKQDFVNGKVGMIFT
                     STADLAGLMQQTEGKFEISTAFLPKNQKYATPTGGANLVMLEGGTDKEKEASAEFIEW
                     MTQTDKIVQFSSSTGYLPTTEDATNSEKLQTLYKEKPQYKVATDQLQYAVALPMLNGY
                     KEATDKLMDEIKKGLTDLNANPKDVVSKGTSAMQAVIDKTNK"
     gene            complement(38024..38884)
                     /locus_tag="L3M60_000760"
     CDS             complement(38024..38884)
                     /locus_tag="L3M60_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBN6225456.1"
                     /translation="MDIIQPIKKDKVDIKKIASKSIEILILLLLSAIFIFPFIWMLST
                     SFKNPQEMMQLPPTIIPNDIVLENYSTAWHSGPFFRYLLNSVFVTGSVLIIQFLVMVP
                     AAYAFSKIKFKGEKVLFGLLLVGLMIPPQVTFLPVYMMMSKFGFINTYVPLIIPFMTS
                     SFGIFLLRQNFMQVSDEIIEAAKLDNASDLKIMFRIMVPMAKPAVITFILFNFIYHWN
                     NYFWPLVMTNTDAIRTLPIGVALLKSSEGITAWNVIMAGNIILILPIILVYIFANKKV
                     KEAFMYSGIK"
     gene            complement(38928..39797)
                     /locus_tag="L3M60_000761"
     CDS             complement(38928..39797)
                     /locus_tag="L3M60_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBN6225457.1"
                     /translation="MKKQFNKAYLYITPAIIGTLLFIVYPIIKTISLSFKSGSLLSST
                     FENVGLNNYTELLKNPEFIQTITNTAIYTFFMVTISISLAIVLAVWLNKNSIIHNLTQ
                     SIIFTPHVISLVSVAVLWLWIMEPQYGFLNWILSTLHLPTSKWLSSESSALMSLILIG
                     IWKTLGYNILIVLSGLKSIPNYIYEASKLDNANKITNFFKVTLPLLSPTIFFLMITTT
                     TASFQVFDEISIMTQGGPVGSTNMLSYYIYESGFIYYDIGQASAASMFLLAIVMIATY
                     ANFRLLSKKVHYQ"
     gene            complement(39781..40851)
                     /locus_tag="L3M60_000762"
     CDS             complement(39781..40851)
                     /locus_tag="L3M60_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225458.1"
                     /translation="MADIRLENVTKSYNKKNTVIENLNLTIEDGSFTVLVGPSGCGKS
                     TTLRMIAGLEDITSGKLKIEENDVTKTDASKRDIAMVFQNYALYPHMTVRENIEFGLK
                     NKKVEKEKREKLITEVIDVVGLREYLNVKPGNLSGGQRQRVALARAMVKNPKVFLMDE
                     PLSNLDAKLRNQMRTELIQLHQKLKSTFVYVTHDQVEAMSMADKIVIMNKGKIMQIGS
                     PKKIYQNPKNLFVAQFIGNPCMNILDIKNDIKFGFRPEKATLESINIDGKNDDVFSLF
                     GKVITKEVLGNETIYCLSVLNSEIRVKTENDIFDIGRTLRISVLEKDLYYFDKNGERI
                     EDLKICKNMYSKIKGDIYEKAI"
BASE COUNT        13064 a         7259 c         5056 g        15915 t
ORIGIN      
        1 tattataaat taaaacaata atttttataa atatcttatt tatattttat aatttatata
       61 tttttacttt agaaattatc aatattacag ttttgctttt aagaaacatc tctatcatac
      121 ataagcatat actctattat gtattagtat aaatatgcct cttcttatct cacataagaa
      181 ctctttataa aagaacctat cataacaaac atgtatacat tatcatatgt atatatcatt
      241 atataaaaag tcaaacttta ttaaaaaaat taataaattg ccttaaataa taccataaat
      301 caaatttgcc atcaaaatca cattaaagta ataaattgat ggcaaatcta atatattaat
      361 aatatataga gtcatagaac gaacagaaca aaaaaactac actcatcttc taatcgtctt
      421 atttattttt gagatatttt taaatttctt ttcttttttt gctaagtatt cgcttttatt
      481 gtctgcaaat actgcctcag ttttaagcct acgataggat tcccatcttt taggcgaaag
      541 ttcttgatga gcaatagctt cccaaatagc acagcctggt tctgaagtat gagaacaatt
      601 acgaaaatga cattttccga aataagcttc tatatctgca aaacttttat ctacaccttc
      661 cgaaacatcc cacattccca actctctcat acctggtgtg tcaataacaa taccaccacc
      721 tgaaattaaa aataactctc gtctagttgt ggcatgtctt cctttatcat cagctatgcg
      781 aatttccttt gtatttaaaa attctccacc cagcagacaa ttaattaatg aagattttcc
      841 aactcctgaa gaaccaagca gagcaatagt ataacctggt ttgatatact tagaaatttg
      901 ttgacaacca tctgactctt tagctgatgt aacaactaca tctacaccca aggctataga
      961 attaactact tgtaatttac gatgtacatt gtcacataag tccgatttgg taagtactac
     1021 taccgggaca gctccacttt cccatgttaa agacaaatat ctctctaatc gatgagtgtt
     1081 aaaatcttta ttcagtgaca tacacaaaaa aacaatgtca atgttagctg caactacctg
     1141 ttcctcattt gcctttccag cagccttacg cacaaacaag ctttttctat gaagcacatg
     1201 atgaataata gcattaccat ttttatcatt acaacggtca agtataacaa aatcaccaac
     1261 tgttggaaaa tcagatggta tagtagcttc aaaacgtagt tttcctgaaa cctctgcaaa
     1321 aactatacca cctgtacata cagcttgata aaggttcttt tcctgtgata taatgcgagc
     1381 aatgtacaaa tcatgatatt tcatactctg agtaataaaa ttactattta gaccataatc
     1441 ttgtaaatca atctctttca taccattcat tctgtttcct ccttctccat cttttcagtg
     1501 attctctcta gtgggaaagg tggttgtgcc agtggtttta atgcaattga cattaatttc
     1561 actggattat catcagctgc aatacggtta gaactaagat gtccacatcg tgtacaacga
     1621 tgaataattg cccattcgcc attttttctc acccacactc caataggttc catattagca
     1681 tcacaatctg cagcacggtc accaggaatg atatccacat gaagactatg taagcaatgt
     1741 ggacaatgat tcctatgttg tgtaccagct ccttcaggta ctacaatgca accacagttt
     1801 ttgcaaataa agttatccaa acaggaattt tcctgataat aagctcgttt tctattttta
     1861 taattcattt ttatcgcctc catatcttgt gttttataca aaaaaatcca caggtattta
     1921 tatctgcctt tttgtgtggt atatatattt gcctacacaa aaacagaaaa ctcctgtgga
     1981 ctagaaaatt taatatcttc tatttcaaca taactatatt caaaataata ctatgtaaaa
     2041 ttaataaaag tgtagcagat taaagcccac tacaccacaa tttaacaaag taatatgtaa
     2101 tattattatt tgttgtcgaa tgttagttat ctataaaggc agagtaagac acaacaatta
     2161 aatgtatcaa aaatacactt aaaaattctg attaaacaga acccatatgc gcctccaact
     2221 atttatttgt tggttaccat tatatacaac tctgacacca ggcaagacac tcctttacta
     2281 ataattatta ttgaaatttt atcataaaat atgagtatgt tcaagtataa tttaacatat
     2341 tcatatattt atgtttatct tattttttta ttctatagaa tctagtaatc ttttgaaatt
     2401 caatattcat tcaatattaa tcataaaaat taaccgctat tatttctttt atcatcttat
     2461 atctctccct caatttatct aatttaagtt aataacaaag gtcttttaaa caaaacgtat
     2521 aatcaagctc ttccatatat tcttacatga atagtatatt tttttatata aaataacgtt
     2581 catatttata aatttattta attttcaaac aagtatattg aataataatc aattctattg
     2641 tattattgta attgtagaat aaaactaaat cggcaaaact agagaaattt agtgacgcaa
     2701 agctataggg actaacgctt ataaaataaa atatgagtta tgtcagccag ttgccaaaaa
     2761 gatatattct ttttgttttt atgaaagttt tttaggggga taataatata atgtttagag
     2821 ataaaatgga tagatgtaca catatgttga ctgcttatat tggtagttca tatgattatt
     2881 gtgattttat agatacacaa ctagatgatt ttatactaga gtacggagaa aatgtagtag
     2941 aatcttgtct gcatcaagtg atggtattag taagtaagta caattaaata gagttggttt
     3001 tctagagaac cctttttagt tttatctact ttaaatagtc ttatgttaga aaactaatta
     3061 aaatagattt ctagtattca tagttttatt ttttatttaa aatacatgta ataaagatgt
     3121 aaatttgtat caaaaaagaa atatgataac ttaaattttc ttatctatat gaaaataaat
     3181 atctcttaaa atgaaattct gtaaacataa atgattttta agtttgaagg attaaattaa
     3241 caaaattata aatttgcaaa taagctatat taaaaaatat aataattttt tatataactt
     3301 attcttttat aacttttaat tttaaaagag gagaatgtat ataagaatat tcaatgtact
     3361 agaaaaaatc aaaagaaata ttagaagaaa tggatattga gtaatcaaag agaagatttt
     3421 gatactacaa atgatatgtt tcaggaaagc attcctatca caacaaaaat tattcttgac
     3481 gatatacctt cacatgatga attaaatcat gttttttcaa aaggttgtga atgaaaaatg
     3541 aaaaagagaa aaattgtatt aatcacatta ctattaatag gtgttttgtt attaggaggt
     3601 atcctatata atcttttttt agtaaaaaca gctaatattt caatgctaaa ggaatcttgg
     3661 aatttggata taccaatacc taacaaagaa attgaagtat ttgatactca agatagtatt
     3721 aatggagatg gacaaagtta ttttattcaa gaattttctg aaaaaaattt caaaaaagtt
     3781 tttaatttaa aaggtggtat agttgtaagc aaagataata ttaatgaaat agaaaaatat
     3841 attgataaat ttaaaagaga ctctgttaac attaataaaa gtaataaaaa taaaattgaa
     3901 gaagatttta aaaaatataa gttagaagtt aaaaaagatg ataagtatat atataagaga
     3961 aattatgaaa actatgtagt tttaattata aaaaaagatg aacaaaaatt atacagttta
     4021 atatggaatc agtaggtaca tatgtattat taagaattat aaataataaa aataaatttt
     4081 agtcaatata caaaagcctt ttaaattgtc tggaaatcac ctcttcattt gttataaaaa
     4141 catacaacaa atgaagaggt gattttatgg tttttaaaaa aataattata agtggatatg
     4201 tgctttcaca tgtttatcaa catttgtaat gcctatattt actaatgaat tataatataa
     4261 aaatattaaa taatattcaa ctatagatta tgtaaattat tctatgaaaa aaatgagttg
     4321 tataaaattt ggaaactgta gattgacgat ttcttttaaa attacaatta taataagttc
     4381 tatagagtta aaaataaaac tttagagatt tttataacat tgtagactaa atttgccacc
     4441 aaaattactt tacagtggca aatcggtggc aaatacaata tattattatc atataaccta
     4501 tataaacatt actattgctt gattatctta gctacagttt ccacatgagt agaatgaggg
     4561 aacatatcta caggttgtgc ctcaacacat ttaaatccgt gaacatttag ataatctaaa
     4621 tctcttgcta aagtagaagg attacaagaa acatagacaa ttctttccgg actcattgaa
     4681 acaatagtct ctaatacctt ttcgtcacat ccctttctag gtgggtctac aactactaca
     4741 ttagcagtct taccctctct atacatctta ggtacaattt cctctgcctt cccaacataa
     4801 aattcagcat tatgcatatt gtttaattta gcatttatct tagcatcttt tatggcatca
     4861 tctataattt ctattccata aaccttttta gccttttgtg ccaaaaataa agatattgtt
     4921 cctatcccac agtaaatatc aaaaacagta tcagtctcat ttaaatctgc atattcaaga
     4981 gccttatcat acaatacttt agtttggaca ggatttactt ggaaaaatga taatggcgaa
     5041 atctcaaaca ctaagtcacc tatataatca tttatttttc catctccata tataactata
     5101 ttttcttttc ctagaatggc attcgtatct ttttgattta catttacaac caaagtctta
     5161 aatccaggta tattttcctt aagaactgaa gctaattctt taagataagg aagtttgttt
     5221 ccattcgcta ctaatacaac cattacttct ttagttgtaa aacctatttt ggttactaaa
     5281 tgcctaatca atcctgtatg agtcttttca tcatatatac ttaccttata agctcttata
     5341 tatgttttta ttattttaac aattttatca tttatctcat gttgtattac acatttttct
     5401 gtcgatatta catcatgagt ctttttttta taaaaaccaa taattggaac accattattc
     5461 ttttgtattg gaaattgtgc tttatttcta tatctatatg gattatccat tcctatagtg
     5521 tcatgaactt ctacatcttc taacttacct attctcgaaa gtacttgttt aacttcattt
     5581 gttttcattt caagttgttt attataatct aaatcttgta tctgacaccc tccacaatcc
     5641 ctgagattgt cactacacac tcttttcacc ctgaatggag acttttctat tatctcaaca
     5701 atatctccta cagcataatt tttctttact ttattaatcc taactttaac tttgtcctgt
     5761 attaatccgc cctctacaaa aactgtaata ccattgtact ttcctactcc tactccgcct
     5821 tgaccaatgt ctactatatc tacaatatac acctcgtttt tcgaaagcat aaatcaccct
     5881 ccaatcaatc tattatattt aaatctttac aaactttttt ctttaaattt atattaaaat
     5941 attgctatta attaaactat attttttact aagctacaat attttatttt accaaaaaaa
     6001 aaggagctta taaagcccct tggtaaataa atttattatt tttcttataa aactctagtt
     6061 gaacctctat ataccacacc tcttgatgcg tccacagtta caacttctcc atcttttact
     6121 agatttgtta tgtcagttgc agacacaata actggtatat ctaaatttat acctacaata
     6181 gctgtatgtg atgtcattcc accattctct gttactacag ctcctgcttt ttctatataa
     6241 ttgttcattt cagcatcagt agcagtagaa acaagtatgt ctccttcatt aaagtctaca
     6301 caagtatctc cattctttat cacacgaact ttaccttcta ctgtttgagt acctacacct
     6361 ataccttgca caatttcttc gcttattaca tgaaccttta ttaagttagt agttccactt
     6421 actcctactg gtactccagc agttataact actaattctc cattatctat ataatcagct
     6481 tgtctagctg cctcaattgc cttttctata acttcatctg tatttcctgc aacagaagac
     6541 ttaactggat atactcccca tgtaagagct aatttattca tagttttttc attatttgta
     6601 gtagctatta ttggagattt tggtctaaat ttagatacca ttctagcagt atatcctgaa
     6661 gaagtagatg ttattatagc tgatgcattt aaatctactg ctgttgtaca agttgcatgg
     6721 cttattgcat ctgttacagt aacattgtta gtaccatttt cctttaataa tctatcataa
     6781 tctaaagtct cttctgttct ttttgctatt gtagccatca tttttactgc ttctacagga
     6841 tattttccag cagcagtttc tccagataac attatcgcat ctgttccatc atatatagca
     6901 ttagcaacgt ctgtaacttc tgctcttgtt ggtcttggat ttcttatcat agaatcaagc
     6961 atttgagtgg cagtgacaac tggctttgct aattcattac atttttttat catcattttt
     7021 tgaactattg gcatctcttc agttggtatc tcaacaccta agtcacctct agctaccatt
     7081 ataccatcag aaacttttaa tatttcatct agattctcta caccttcttg gttttctatt
     7141 ttagatatta tttgtatatc agtcgcatta ttgttctcta atacttctct tattgctagt
     7201 acatcagatg cctttcttac aaaagaagca gctatataat ctattccttg acttattcca
     7261 aattctatat cgcttatatc tttaggagtt attgctggta aatttatttt aacaccaggt
     7321 acatttactc ctttatgatt tttaactatt cctgaatttt caacaacaca aattatatct
     7381 tctccattta tttcttttac tcttaagcca actaaaccat catctattag tatagaatct
     7441 cctgatttaa catcttcagc aagaccttta tagcttactg tacacatttc tttagtcccc
     7501 ataatatctt tcatagttat agtgaacttt tgaccttctt ctaaaaatac ctctgggtct
     7561 tcaaagttac ctgttctaat ttctggacct tttgtatcta ataatatagc aactggctta
     7621 tttaattttt ctctaacttt ctttgccata tctattcttt ctttatgttc ttcatgtgaa
     7681 ccatgagaaa agttaaatct acacacattt agaccatttt gcataagttg agttaaaact
     7741 tcctcacttt gtgatgctgg acctagtgta caaacaattt ttgtcttctt tacattattt
     7801 aacatatttg ctcctcctga gatattattc cacagattta tttttaatac taaaacttat
     7861 atagataata tttttgccat ctcataagct tctttatcaa atactttctt ttgagctaaa
     7921 gcttcactta tttctaagtc aattattttg ttttctttta ttcctacgac tctagctgat
     7981 tttccatcta ataataattc tactgctctt actccaagtc tacttgctag tattctatca
     8041 gatacagttg ggcttcctcc tctttggaca tgacccaaca cagttactct taaatctgct
     8101 cctgactctt ttttaagttc cttttctaaa tcgctggcac ttccaactcc ttctgctaaa
     8161 acgattatac tatgtctctt acctcttttt tgagtagtct ttaatcttaa agcaacttca
     8221 tctactgtaa tttctacttc tggtactatt atagtttccg ctcctcctgc aagacctgca
     8281 tataatgcta aatctccaca gtgtcttccc ataacttcta ctatattaac tctttcatga
     8341 gacgatgatg tatctcttat cttacctata gcatctataa tagtattcat tgcagtatca
     8401 aatcctatag tatagtctgt atatgctaaa tcgttatcta tagttcctgg tatacctata
     8461 gctgggaatc ctaagtcact aagtttttga gctcctacga atgaaccatc tccaccaatt
     8521 acaactaagc aatcgatttt gtacttttta agaattttta ctgcttttaa tctaccttct
     8581 tctgttttaa attcttcaca tctagaagat tttaatattg ttcctcccct gtgtataata
     8641 tctccaacag aagataggtt catttcagtt aaatcttctt ctagtaatcc cttataccct
     8701 ctattgattc catatacttt gcatccataa tatattgctg atcttacaac cgctctaatc
     8761 gcagcattca ttcctggagc atctcctcca cttgttagta atcctatagt tttcattttt
     8821 tacctccttg gattttatgt caatttttta ttataatcat tgttattgta ttctaatgga
     8881 ctttttttgt ccctatatca ctttttaatc ccatcaagac taaaaaaatt aaatgtcctt
     8941 aaatgtccat tttgtgatat tatatcacaa aacggacaat atttttcata ttattataat
     9001 atatttagat ttttatataa aaaatcaagc ttttactttt atgttatctt taggaagtaa
     9061 ctcttctaag ttatttataa attcatcaca tatacaagtt aatgcccctg tcattttata
     9121 tgctttacgt ttgccattta tattttcatc catagggtac aaatatatac tgtcattacc
     9181 cggatattgt tttgctattt ctacaatatt attagttgta tcggtgtcag acatactatc
     9241 tattttcaaa tacaattttt taccactttc tggcttaata ttttccacat ttctagttct
     9301 tcctgaattg taatttctat ctacaaagtc agtatcatca tttatatctt ttatctctct
     9361 agctataagt tttgcatttt cgccttcttt tatacttaat gttcctttta catatattat
     9421 attatcttca ttcaaaatga cattgtactt ttgtaataat tgaggaaaaa ctactatttc
     9481 tatagcacca tacaaatctt ctaattcaag aaatgccata atttcatttc gttttgttgt
     9541 tttaatagtt ttagttacaa tcattccacc cattataaca tctgtttcat tcatttgcag
     9601 gtatgactct tcttcctctt ttaatgaatt taattttcca ttatccatag atgttttttc
     9661 ttgtaattct ttttcaaatt gtgacaaagg atgtccactt acatacattc ccaagacttc
     9721 tttttcaagg ttaagtctct ctctctcttc gtactcttgc cttttaggca aactatatat
     9781 ttgttgaaaa tcactatctt cctcactatc tccaaaagca tcaaataaag aaatttgacc
     9841 ttgaacattt ttctttctat ccatagatac actttctaat aaactttcat aaccagccat
     9901 caaactagct ctattttcac ttatattatc aaaagctcca cattttatta gactttctat
     9961 tactctctta tttgtatctt tttggtctaa tcttttaact aaatctacta aatctacaaa
    10021 taatccatta gattctcttt cagcaattat attgtttatt atatttacgc caacattttt
    10081 tacagctgct agaccaaatc ttatattgtc accttcaact gaaaactttg aaaaactttt
    10141 gtttatatct ggaggtaata catctatttc tagtgcttta cattctctta tatactcaac
    10201 taccttatca gtatttccca ttacactggt tatcaatgct gccataaatt caactggata
    10261 atatgtcttt agataagctg tctcataggc taaaacccca tatgcagctg catgtgattt
    10321 attaaatgca tatcgagcaa agtctatcat gtcatcaaat attttattgg ctatttcctc
    10381 ttctacacca tttctgatac accctgcaat ttctatatta ccatcttcat caaatttccc
    10441 atgaataaaa tattgtcttt cttcttccat cacatccatc tttttcttac tcatggctct
    10501 tctaactaag tcacttcgtc cgtaactata accacctaaa tctctaacaa cctgcataac
    10561 ctgctcttga tacaccaaac aaccatatgt aacttccatt ataggcttta atttttcatg
    10621 taggtatgtt acatcttttg gattgctttt attttttata tatgcaggta tagaatccat
    10681 tggacctggt ctaaataggg atattcccgc tacaatatct tcaaaattgt ctggttttag
    10741 ctgtttcata aaacttctca tcccagcact ctccaattga aacaccccta atgtattccc
    10801 agatgaaagc atttcatata ctttcgggtc gtcatagtcc atctttgaaa aatctatttt
    10861 ttctgtgtag ttttttataa ctctattttt ttctattaaa tcaagagtat ctcttataac
    10921 tgtaagagtt cgtagcccaa gaaaatccat ctttaaaagc ccaagttctt caagtgttgt
    10981 catagtaaac tgtgttgtga ttgcatcttg atgcttgtat aaaggtacat actcatctac
    11041 aggatttttt gatataacaa ctcctgctgc atgagtagaa gcatgtctta acataccctc
    11101 tatctgtttt gatatattta ttatttcttt tgtttctggg tcttggtcat acaattcttt
    11161 aagattagga tttgtatcta aagccttatc tattgtcatt cctaaagcaa atggtatttc
    11221 ctttgctatc ttgtcaactt tattgtaacc tatatccaat actctcccaa catctcttat
    11281 tgcagctttt gcacccattg ttccaaaagt tattatttga gctacatggt catcaccata
    11341 ttttctcttt acataatcta taacttcttc tcttctctca taacaaaaat ctatatctat
    11401 gtccggcatt gatatacgtt ctggatttaa aaaacgctca aataatagag aatattttat
    11461 tgggtctatg tcagttatct taagtgtata tgctactatt gaaccagccg cactacctct
    11521 acctggccct actatgatat tattttcttt agaaaaattt ataaaatccc aaacaattaa
    11581 aaaatactct acataaccca ttttttcaat tacatctaat tcataattta atctctctag
    11641 tatctcatca ctaggattat catacctctc ttttaatcca ttaaaacaca actctcttaa
    11701 ataggtttct ggtgtatacc cttctggaac atcatactta ggaagatgta tagtgttaaa
    11761 atcaaactcc acattacaca tatcagctat tttcacagta ttgtcaatag cctctaatgc
    11821 atatggaaat aattcttcca tttcttctct cgattttaaa taaaactcat cactaccaaa
    11881 tctcattcta tttgggtcat ttaatgtttt tcccatttga atacacataa gaacatcatg
    11941 tattttcgaa tcttctttat ttacatagtg aacatcatta gtagcaacta gtggtatacc
    12001 agtctctttt gatagtttta ctaatccaga gtttacttct ttttgctcag gtaaattgtg
    12061 atcttgaatc tccaaaaaga aattttcttc tccaaatata tctctgtagt caaaagcaaa
    12121 ttgttttgct ttttcataat ttctattcat taaagcacgt gctacatctc ctgcaaggca
    12181 tgcagaaagt gctataatcc ctttgttatg ttttttaagt tcttccatgt caactctagg
    12241 cttatagtaa aaaccttcta cgtatgaagt tgaaactatt tttattaaat ttttatatcc
    12301 ttccatattc ttagctaaca gaactaaatg tccttgatat ttatcatagt ttgggtcttt
    12361 atctctaagt ccacgtgcag ctgtatagac ttcgcaccct ataatcggct ttattccttc
    12421 ttttatagcc gttttgtaga aatctataac accaaacata caaccatggt cagttatagc
    12481 tattgagctc atgtttagtt ccttagccct ttttataagt tttttcaccc tcgaaaaccc
    12541 atcaagtaaa ctatactcag tatgtacatg aagatgagca aaatctttgt tcattaaatc
    12601 acctcctgct ttataatttt accacaaata tatgtcctaa aacaaaaaca gagactttta
    12661 tctcaaaata ggaattaact tcaacatata ccattatatg ttataaatta atgcttattc
    12721 tgagattgtc ccctcaattt attgtgatga tttttttttc aaatctgtcg caatttcttc
    12781 tatttttcta agcctatgat tgactccaga ttttcctatt ggtggttcta acatctctcc
    12841 aagttctttg agagtcatat cctcatactc gattctaatc ttagctattt cttgtaaatt
    12901 ttctggcaaa ctgctaattc ctatagtttc ttgtatgagc ttaatatttt ctacttgtct
    12961 tactgctgca tttactgttt tagaaaggtt agctgtctca caatttacta tcctgtttac
    13021 attatttctc atttctttta ctattttagt attttgaaga cttaaaagag catggtgtcc
    13081 tcctataata ctcaacaaat ctgatatttg ctcactctct ttgatataaa caacgtgatt
    13141 attttttcta gccacaattt tactattaaa tcctaaagag tttataagtt cttttagaga
    13201 atctgcaaac tcctcattat tagttacaaa ttccatatga tagttctttt ctgggtcgct
    13261 tatagaacct ccaccaagaa aagctcctct tatgaaagct ctcttacatc cttcatgctt
    13321 aattaagtta tctgaaattt tgtttatagt aaaaaatcct tctttatttt caagtatacc
    13381 taactttttt aacaacattt ctgaacccat ttcacttgtt accattaata cataactgtt
    13441 atttctcttt agcatttgat tcttatttac cgagattgta gtattgatat taaaattctt
    13501 ctttaataat ttaaatactt tacgggctat tgaatttaac tctgttgtta tctttagatt
    13561 taacttctta tatccaacaa tttgtatact tccccctgat ttaactagag ctgacaattc
    13621 agctatatta caacattcat tcccagatac aactctcgct agttcatttt tggtttcagt
    13681 tgaaaaagac atatctatca cctattaact aatttttctt taaccttttt tttatttctt
    13741 gtaaggcaac ctttattatc tccttttcat catcatacct agctaggtct actactctat
    13801 ctagatggat aaatttagca tcaataaagc cttcctcttt ttgtggaata gtatccatat
    13861 tctttgcttg catcaaatac caaaaaactg ttttatgaac tgctctattt tcatcccagt
    13921 tttctttgaa tgtatagtgt atttcaccta aatatttcaa tatatctgct tttactccag
    13981 tttcttcact cacttcacgt aatgctgcct cttggttatt ttccccttct tctacttttc
    14041 ccttgggtaa cacccaatct ccattgaact tccttagtaa aagaattgta ttgccaaaca
    14101 ggactacacc accagcacta acctcttctc tcatcgatgt tcactcctta aaactaacat
    14161 aatgataagg atttctacac aattttttaa aatccttttt tactaacttt tgttgtaatt
    14221 atgatttagg gctaactcaa taactatgtt agatatatac tttgcatcat gtcttatata
    14281 attatttttt atttcaatta aattcttctc tacagttttt atacccatct cttttaattt
    14341 atctttttgc tttttatcta acaaaacttg ttctgctcca tcttgtttgt ataagtcaag
    14401 catttcttct ggcagtatct cattatttgc gattacataa tctattaaat tcttatcagt
    14461 atgctttact atagcattaa catgctctaa tacattgtac ccttctgtct caccgggttg
    14521 agtcattata tttgctatat atacttttgg agctacagac gatttaatag cgtctacaat
    14581 cccctctaca agtaaatttg gtattatact cgtatataag cttccagggc ccataattat
    14641 aatgtcagca tcgtaaatag atgcaataac atcctttaat ggtttcacat cttttggatt
    14701 taaaaaaatt ttatcaatag agcttttttg aatttttgac tcttcaggta tactagattc
    14761 tccatttatt atattgccat tttctaattt ggcaactaaa tttacatctt ccaaggttac
    14821 tggcaaaact cttcctgtta tagcaaaaat ctcacttaat ttatatacag ctttttcaaa
    14881 gtttccatac aacccattca tagcagccaa aaacaaattt ccaaaactct gaccttttaa
    14941 aagcccttct gtaaacctat attgcataac ttcattcatt gttggctcta tatttgcaag
    15001 tgctaataga cagtttctta tgtctcctgg tgggagcatt cctaagtctt ctctcaatac
    15061 acctgaacct ccaccatcat cagcaacagt tacaattgca gttatatttt gcgttgtgtg
    15121 tttaaggcct cttaaaaata ctgattgccc agtacctcca cctattacaa ccacatttgg
    15181 ttctctattt tcaacaatag acctttgaaa taattttact ttttcatttt ttgctttttt
    15241 atatataaat aacgatataa ttaatgcaat ccaacctaaa attcctacaa taataagtaa
    15301 tttagccatg tttgcctcca ttatcgcaac atagagtctc tatgtttctt tacaactcta
    15361 tatcccttat tagataaatc ctccgctata agatttgtta tagtaactga cctatgtctt
    15421 ccacctgtac atccaacacc aattactaga tgttgttttc cttcctctat atattgaggt
    15481 actaaaaaat gaatcatatc taaaagtttc acataaaatt cttcactgat tttagaattc
    15541 ataacataat ctctaacttc tttatcatca ccagttttag ctcttaactc ttcaacataa
    15601 tagggatttg gaagaaatct tacatcaaat actaaatctg catcagcaag tataccatgt
    15661 ttaaatccaa aagatactac agaaatagtt aggtttggat tatcttcacc agaagaatat
    15721 attttcttta tttcttcctt aaggtctttg ggcttcatgt tagttgtatc aattatacat
    15781 gtagataatt cctttaaagg ttccattatc tttctttcca tttttatacc ttctggaatc
    15841 tgcatatctt ttgccaatgg atgatttctt cttgtcattt tatatctctt taacaataca
    15901 tcatcattac aatccaaata taccatttca tattcataat tttctttttc taaataattt
    15961 aagctttcat gaagcgcttc aaaaaacttt ctccctctta tatctatccc tagagcaacc
    16021 ttatcaatac ttgaatttgg ttgataacat aattctgcaa atttagttat caaagcagga
    16081 ggtagattat ctacacaata aaatcccata tcctctaaag ctctcattgt ctcacttttt
    16141 ccagatcctg aaagacctgt aactataaca aatttcattg ctaactcctc ccttttatct
    16201 tatgtttatt atattataat gttttctact aacgatatat ccagattaga gtcagatata
    16261 gtctttatag ctagttcaaa atcgccaact ctttctggac aatgtgcatc tgagcctatt
    16321 ataaatttat tttctagccc ttttatctca tttaattgat ttaagttcaa aaaagaatgg
    16381 ctactgttta tttcaagtct agtgtcagtc ttttttgctg tctttgctat ttcctctata
    16441 tacacatcgc ctttatctcc tggatgagta attataaaaa tatcattatt acttatcgca
    16501 tttgctacag cttttgtatt atattctttt gctttttcag ttttaaaaat atagttatta
    16561 caatgattta ataaacttct taatgatgtt ggagtagaac caaaatgata tccagccata
    16621 atatagtcaa aatatctaat gaacttatca tccatatcta tattgccttt atcgtctaat
    16681 atgttacact ccatacctaa aagtatattt atatcattgt acttatcatt taaataatct
    16741 acttcttctc tcattttaag aatatctttt ttctttacac catatgcaat atgtttataa
    16801 ctatggtcag atattcctat tgtttttatt cctttagaaa tggccacctt tacattatcc
    16861 tcaatagtcc ctttgccatg actatataat gtatgtgtat gataatcagc cagtattttc
    16921 atttgttaat cctctccaag tattttgact tcttcttcca acataacccc aaacttggca
    16981 tatacagcac tttttataac atatattaaa tccaaaatat ctttagcact tgcttctcct
    17041 tgatttacta caaatccaca atgcttctca gacacttgtg ctcctcttaa agtaagacct
    17101 ctcaatccag tttcttctat taatttacct gcaaaatatc ctgttggtct cttaaatgta
    17161 cttcctgcac tagcaaaatt taatggctgt ttagttattc ttctcgtcgc taaatcatcc
    17221 attaaacttt ttatttcatc ataattgcct tcttgtaatt ctaattctgc tgataaagct
    17281 atatatccat ttttcgatat tatacttttt ctatacccaa attccatctg tttatttgac
    17341 aattcaaata tattgccttc catatccatc actcttactg actttactat gtccttcatt
    17401 tcaccaccat atgctccagc attcatggct aatgctcctc ccaatgtccc aggtattcct
    17461 gatgcaaact caaacccttt taattcttgc ttttggagag ctttacctaa aactgataat
    17521 aaagctcctg actgagcagt catcctagta tcatttatct cataagagtt aaaattatca
    17581 gctaattcta ttaccactcc tcttattcca ccatctttaa ttaaaatgtt tgaaccattt
    17641 ccaattatta aatacggaat agattctttt tttaccaact taagtacatt ttttaattgt
    17701 tcttctgttc taggtctaac taatatatct gctggtcctc ctaccctaaa tgaaatatgt
    17761 tttttcatag gttcatcgac ttttatatcc tctttactta atatatttaa taaatttata
    17821 tatatatctt gattattcat tgtatttcct cccaaattat actgaacttt agctgtcatt
    17881 attatttaaa ttatattagt atatagtatt aactatattc aataatatat gattttgctt
    17941 atcatatgga ttttatattt taagcttctt aattacaaac tatattcaat aaatatataa
    18001 taaagtaaaa aaaaaaaaat aacaacatat aaaagaatat aaataattta aaaaaagcat
    18061 caatatcact gatatcaatg ctttaaaagg caatttaatt catattattt atctaactat
    18121 ttttgacata gcaaagataa taaatccctc caactaccat ggaacctcca acgatatttc
    18181 caagcgttac aaataccaaa ttagttatga attgaaccat agttatattt gctcctagtg
    18241 ccattcccat tggtataaaa aacatgtttg caacactatg ttggaatcca cataatacaa
    18301 atagcattat tactgtccaa catgaagcca attttgacat tatatcttga gatgctgtag
    18361 acatccatac agctaaaacg accagaatat tacaaaggat tgcttttaaa aacattgatg
    18421 ctatagttga attaacctta gcttctgcca cagctatagc ttttgtagct gcatctcctg
    18481 tcaatgtaga agaataaaac acaagtgcga ctaacaaaat tgaacctact aaatttgcaa
    18541 aataaaccag tccccagttt cttaacattt ttcctaatgt tatttttttg tccataagtg
    18601 caatagccat taaagtgttt ccagtaaaca actctgctcc tgctactact acaagcataa
    18661 gacctactgg aaagaccata gcacttaaaa agctagctac ccctgggtct atcttgccaa
    18721 aagtttgact aactatcata ctccccaatg ccccaaaacc tataaatgct cctccaaata
    18781 aacctaataa aattgcattt tgtgtagaaa tgtttgcttt tttaatgcct gcacttatag
    18841 ttgcttgtgc agtttcacca ggtgtcaaga atcctttact cataaatcct tcccccttaa
    18901 aaattatact ttcccttgtc tttttatggc aataattata taataacaat atgatatata
    18961 ataaatttta ttaacataaa aaatatacta tatgcttata tatacttgtc ttatataatt
    19021 atactattta ttatataaca actttgttaa tttcgaaata atttatattg aatttgaaaa
    19081 atttaccttt tgattattta ttttactttt ttattattga ggtattatat ataatagata
    19141 tatgtttgta ttgttatata taattactta attttaataa atatttaaag gaggaaattt
    19201 tatgtttgat atatcatttt tcgaaatgtc aatatatgaa atagcagtta ctacaatata
    19261 tataattaat ttcatggtta tactaaacct tatctttaga gaaaaaagaa acataaatac
    19321 aactttaaca tggcttctta tattagtatt gattcctgct cttggattca tactttatat
    19381 agcttttggt agaaatattt caaaaaacaa catgtttaga ttgaaagaaa aggatgataa
    19441 gataatcaaa agcaatatac tagatactca ggttaaactc caatctacat ctgaaattga
    19501 ttcagatata catcaacata aggatatgat ttatgcattg gctaattcaa ataatgctca
    19561 ttataccaat aataacgatg tgtggattta tgctgaatct agtcaatttt ttaacagtct
    19621 tcttgaagaa cttaaaaaag ctaaaaaata tataaacata caattttaca ttttcaaaga
    19681 tgacaaaata ggaactgaaa taatagacat tcttatagac aaagctaaag aaggtgttga
    19741 agtaagactt ttatttgacg cagttggcgg tagaacacta aaaaatagta cactttccag
    19801 acttaaagaa agtggcgtta aaattggaag cttcttccca tcattcttga aaattgtaaa
    19861 ttttaactta aactatagaa atcaccgaaa aattgttgtc attgatggta aggtgggatt
    19921 tgtaggtgga tataatgttg gtgatgaata tttaggtaga aatccaaaat ttggtctatg
    19981 gagagataca catactaagc ttactggtga ttgtgtaatt gacctaaata tgagattcat
    20041 cctcgattgg agatacacaa ctaaagaaga tttagattta gaaaagtact ttatagaaga
    20101 agatacaaat tctaatcatc cttctgaaaa tattgggatt cagatagtat caagtggtcc
    20161 agatatatca gaacttgatg agattaaata tggttacctt aaaatgattc agaaagctag
    20221 aaagtatata tacatacaga gtccttattt aatacttgac tcaactttta tagatacttt
    20281 aaaaatagct tgtttatctg gtgttgatgt gagagttatg attccttcta aacctgacca
    20341 tccatttgta tactgggcat catattcata tgctggggag cttttaaagt ttggagctaa
    20401 aatctacact tatggtcaaa acgcattttt acatgctaag acaatagtta tagatgactc
    20461 tatatgctct ataggaacag ctaatatgga tatcagaagt tttgagctta atttcgaagt
    20521 gaatgcattt atgtactctt caaaaaaagc attggaacaa agaattatat ttgaaaatga
    20581 tattttaaat tcaaaggaaa taacattaga tatatataat tctaggtcta cttatataaa
    20641 aatcaaggaa tcaatttcaa gactattatc agcagtttta taaatctttt attttataaa
    20701 aattttacat tttggtttat tttttaaagt tttttatata tattattttt tttttattga
    20761 atttagctca ttttttttat tttttttaac tattctgaat tattttatat tataatagac
    20821 tagtatagtt tttattttta ataggtaact tttttgcatt gtttttataa ataaagaaat
    20881 tgttgtatat tattctttct ttattgtttt ggttttgttc acccacataa tgttggggtc
    20941 aactcatggt taattatttt ttgaaaaata ataactttaa aaagaaaagg gggatttgtt
    21001 atgtgcgatt ttatttcaca caacaagcaa ttatttgacg aactagatat atttattgac
    21061 tctcttgcaa caaaggaagg tgcgttaata caagttctac atgaagccca aggtatattt
    21121 gggtatcttc caaaagaagt tcaacttcat gttgctagaa aacttggagt agctcctgca
    21181 aaggtatatg gggttgttac attctattct tattttacta cagaacctgt aggtaagtat
    21241 aaaatcagtg tctgtctagg gactgtatgt tttgtaaaag gtgctgacaa aatcttaagt
    21301 gcttttgaaa aacagcttgg cataaaagtt ggagaaacta ctagtgattt taaattttct
    21361 ttagaaggtt taagatgttt gggggcatgt ggtttagctc cagttgttac tgtaaatggc
    21421 aaagtttatg gtaaagtgaa acctgaccaa gtaagcgaaa tattagatac ttatagagaa
    21481 ttagaattga attgttaaga ggggtgaata aataatgcgt aaagtaaatt catttgatga
    21541 attaaaagtt ttagctgatg agctgaaacc taatcttagc ttaagaaaaa attattctaa
    21601 ggaaaatgta ctaagaagag aattacttgt ttgttgtgat actggatgta catcttcaaa
    21661 cagtctagaa atagttagtg aattagaaaa tgaaataaaa aaatcaggaa tccaagataa
    21721 agttagtgtt cgtttgacag gttgttttgg tttctgtgct caaggtccta tcgtaaaagt
    21781 ttatcctgat aacgttttct acgtaaaagt tgagccatct gatgctgaaa agatagttca
    21841 aagccactta attagaaata cagttgtcga ggaattatta tatgaggaag aatctttaag
    21901 taaaaaagta gaagaacaag aagaaatgtc tttctacaaa aaacagttac gtatagcttt
    21961 aaaaaattgt ggtcttatag acccagatag tatagaagaa tatattgcca atgatggtta
    22021 tttagctcta ggcaaatgtt taacttctct tactcctcag gaagttattg cagaggtaaa
    22081 aacttctggc cttcgtggta gaggtggtgc tggtttccct acaggctcta aatgggaagc
    22141 tgcttcaaaa aatccagctg gtccaacaga taaaaagttt gttgtatgta atgctgatga
    22201 aggtgaccca ggtgcattta tggatagatc tgtacttgaa ggtgaccctc atagtgtact
    22261 agaagctatg gcaatatgtg gatatgctat aggttctgat acaggataca tatatataag
    22321 agcagaatat ccaaaatcta tagaaagatt aaaagtagct atagctcaag ctgaaaaata
    22381 cggtttactt ggtgaaaata tcttaggtac tggatttaac tttaaattag aattaaaata
    22441 tggtgcaggt gctttcgttt gtggtgaagg tacagctcta atgcattcaa ttgaaggtag
    22501 acgtggtgaa cctagaatga agacttatag ttcttctaag agtggtctat ggaaatctcc
    22561 tacttgctta aataacgttg agacttttgc taatatacct gcaataatac ttaaaggtgg
    22621 agattggttt gccaacttag gtactgaaga ttctagtggt actaaagttt ttgctcttgg
    22681 tggaaaagta gaaaatgtag ggcttgttga agttccaatg ggtactactt taagagatat
    22741 tgtatttgaa attggtggtg gaatccctga aggtaagaaa tttaaggctg ttcaaacagg
    22801 tggcccatct ggtggatgta taccaactga acatttagat actcctatag actttggttc
    22861 attaagttct atcggttcaa tgatgggctc tggtggtatg cttgttcttg atgaatctga
    22921 ctgtatggta gatatagcca aattcttcct tgagtttact gttgaggaat cttgtggtaa
    22981 atgtacacct tgtcgtatag gtactacaag attgttagaa atattaacta aaattacaga
    23041 aggtaatggt actctacaag atttagatga cctagaaaac cttgctgaaa ctatacaaac
    23101 tgcttcatta tgtggtcttg gtaaagctgc tccaaaccct gttttaagta ctcttcaata
    23161 tttcaaagat gagtatatag ctcatgttgt agataagaaa tgtcctgctg gtaaatgtca
    23221 gaacttactt tcttacttta taacagatga ctgtaagggt tgtacaaaat gttctagagt
    23281 gtgtcctgct ggttgtataa caggttctgt taaagaacaa catacaatag atacttctaa
    23341 gtgtctaaaa tgtggtgctt gtatagataa ttgtacattt aacgctataa tcaagaaata
    23401 acttatttgg aggaggaata acaatgagtt tagttaattt aactattaat ggcaaacacg
    23461 tttcagcacc tagtggaact tctatcttag atgctgcaaa attaataaat ataaaaatac
    23521 acaacctttg tcatttgcat atgaatgaaa tagataagct tgatacttgt gcttcttgtc
    23581 gtgtttgtat ggttgagaca gagagaggat tagttccagc ttgtggaaca gttataaagg
    23641 aaggtatgaa agttcagact aatagtgcaa aagcattaaa tgctcgtaga actattgttg
    23701 aattattact atcagaccat ccacaagatt gctttgtatg tgaaaaaaat ggagattgtg
    23761 aattacagac tatagctgct gatttaggag taagaaaaat aagatatcaa ggttctaaat
    23821 cttttgcagg taaagatact tctactaaat ctttagttaa agaccattct aagtgtatat
    23881 tatgtagaag atgtgagact gtatgtaatg acatacaaac agtaggagca ttatctggag
    23941 ttaatcgtgg atttaatact ctagttagta cattctttaa ctctgatatg gttgaaacag
    24001 agtgtacatt ctgtggtcaa tgtatatctg tctgtccaac tggagcatta acagaagtgg
    24061 ataatgtacc taaactttgg gatgttttaa ataaaaaaga aaagactata gtagtgcaag
    24121 ttgctcctgc tgtaagggtt gctattggtg aagagtttgg attagaacca gggtcaatct
    24181 ctacaggaaa aatggtagct gctttaaaag cacttggatt tgaacatgta tttgatacaa
    24241 actttggtgc agactttact ataatggaag aagctactga gtttatagaa agaatccaaa
    24301 aaggtgaaaa tcttcctata ttaacaagct gttgtcctgc atgggtaaac ttcttagaac
    24361 acaattatcc agacaagtta aacttagcat ctagctgtaa atctcctcaa ggtatgtttg
    24421 gttctatagc taaaaactac tatgcaccta aaatattagg aatcaatcct gaggaacttt
    24481 atgttgtatc tgtaatgcct tgtgtagcta aaaaatatga agcttcaaga gaagaattaa
    24541 gtgaatctgg aatactagat gttgaccttt ctataactac tagagagtta gctaagatga
    24601 taaaagaagc tgctatagat ttacctaatc ttcaagacca agattttgat aatcctctag
    24661 gaaaatctac aggtgctgca tctatatttg gagcaagtgg tggagtttta gaagctgccc
    24721 tacgtacttc atatgagaag ataactaata agactttaga taatgtaaac ttcactaatg
    24781 ttagaggtct taaaggaatt agagaagcaa gtatagatgt tgatggaact acagttaatg
    24841 tatgtattgt aaatacactt aaaaatgcta gaaagattat ggataaggtt agaagtggag
    24901 aatgtaaata ccatatcata gaagtcatgg cgtgtcctgg tggatgtgtt ggaggtgctg
    24961 gtcaacctta tcaccatggt aatactgaaa tagttgatag acgtgcaaat gcgttatatg
    25021 aaatagatag aaataaggct attcgtaaat ctcatgaaaa tcctgattta caagctattt
    25081 ataaagactt ctttggagaa ccaaatagtg atgtagctca taaatacctt catactcatt
    25141 actttgataa gagttgtatt tatggagagt gtccacaaga atgtgcttgt gaagaagcta
    25201 agtagtctta acaaagtata aaatttattc ttaaaaaagg attgtcttaa aatactttta
    25261 tgtaatttaa gacaatcctt attaatttca taaattaaat tatgtttcta aatagatgta
    25321 tttattaatt agcctattta ttaattaaag aatccctgcc attctcataa gttgttttgc
    25381 atttgtagtt ttacatcttc catattttat cttatagtca aaatgaattt tatcgtctac
    25441 atagtactct gagaaatgat agtttacaac ttcaacatct ccattccctg aaaggtcaca
    25501 tagctcaaag tcatgtgttg tcaccatact tataacccat ggtcttgata atttctttac
    25561 tgcctcactt gctccaataa tcctatcagc agagttagtt cccttaaaaa tttcatctac
    25621 tagaaccaac attggcagtt cttttaaact atattgaatc attgatttta ttctaagaat
    25681 ctcagcatag aaagttgata ttccttgact tacatcatct tgtattctca tagaggtaaa
    25741 tatagccata caggttgcat caaagctctt agcacatact ggagcacctg catatgccag
    25801 cactaagttt acacctatac ttcttaaaaa tgttgttttt ccagacatat ttgaacctgt
    25861 gataatacaa gtctgagaat ttaattgtat tgaatttgct attacacttt tttctgctat
    25921 taatgggtga tatacttctt caactttcaa tcttggagta tcttcatatt ttattactgg
    25981 aaagcatgta ttttccttca catgagaaat aactgttaaa cttattagag cttcaaattc
    26041 cccaatagat tcaaaccaat ctctaatttg atttccatat acacctttcc atcgctccaa
    26101 agcttctgca caatgatagt tccacatcaa aacaccacaa gcaattatat atattatctg
    26161 attatacctt aggtttacgg catttcctat tgaatttaat tgtttaacac ctcttgaaac
    26221 tccacttccc ttagagagtt tgtcttgtaa ttctttcaaa tatacactct caaacgtctc
    26281 tttttctaaa acctcaaaca ttttttcata tacttgtata cttctactaa atgaaaataa
    26341 tggagaaaga gtttgcataa gttttggata tccaaagcca ctaaacccta actggattat
    26401 aaaaagtaaa aaaacaggta aaacaggtaa aaatcctaac acacaaaata taaaagataa
    26461 aattatcgca gttggtaaac cccaagtaaa aatatgcatc cattttggta tatacacatt
    26521 cttattttct ccatactcta taaacgattc tatactacta tctgaattgc ttttttgctc
    26581 tttaccgatt atattactga gtgtttgaat atgaatagaa aaatcatctt ttgataaaag
    26641 ttcttttata gccatctgtc tttctatgat tacattctca tctggatttt cattccacaa
    26701 atatttggct aaaaaatttt tcccataaga tgtattagct acacagatat attgatacaa
    26761 tgatgctctt ccaaataagt ctaaatcttt tgcttgcgag ttttcatctt ttaagtattc
    26821 tttaccatct attttaaatt ccttccaacc atctccaaat ctcttaatat acttatttaa
    26881 tacttcgctc ttacttttaa aatatgtttc ttcttctttt actttactat gaataactaa
    26941 gagtgctata aatattatta ttgatacaac tccaacaaaa atacctacta tatttttatt
    27001 gtaaaatcca atagcaaacc ctgctatcac tatcaaaaaa gatattaatc ttgctgtact
    27061 tatgttattt gactttttac ttaagagcaa ttctttttca gtactacttt ctaaaagttt
    27121 actaaaatat tctattccca taaaaaattc caactttctt gttatataat gaaattcaaa
    27181 tataaaatta atctagtatt attataatct aacgctgtac tttaaattat ccttatataa
    27241 tatatcttat taataaacat tatatatata caacttgact ttaatttata aggtgtagga
    27301 cataatccta atcaatattt aattaaatga tgtttagttc attattattc agtactactt
    27361 agtatcaatt agttttgata attttttagt aaattcttga gctgcgtcgt atcccattcc
    27421 tctttgtcta aaattcattg ctgctacttc taatatcata gcagtatttc ttccaggttt
    27481 aacaggtaca accaatttct ctacattaac accaagtatt tcttcatatt ctttatccag
    27541 cccaagtcta tcatagtact tagtttcatc ccaagcttct aaatgaacta ctaaatctat
    27601 cattttagaa tttttgattg cccctactcc atataagctt ctaacatcta ttatccctat
    27661 tcctcttatc tcaagaaaat gccttatcaa ttcaggtgac tgacccacaa gcatactttc
    27721 atctaatttt cttatttcta cagcatcatc tgcaactaat ctattacccc tttgaatcaa
    27781 ttctaatgct gtttctgact ttcctatact actttcacct tttatcagta cacctatacc
    27841 ataaatatct actagcactc cgtgaattgt tgtgtgtggt gctaacttat tatccaaata
    27901 atttgacagt ctatttataa gtctagtagt atttctattt gtgctcaaaa gtattctttt
    27961 atatttttta gccttttcaa ttacatctgg cttaatttcc aagtccctag aaattattaa
    28021 cgctggtatt tcatatgaga aaaatctatc tagtacttct tctcgaatat gactatccat
    28081 tagtccaaaa aaattatatt ctgtctttcc aactatttgt attctttcat atgcaaaata
    28141 atcaaaatat cctgcaaact gtaatcctgg tctatttata tcttgagagt agacataata
    28201 atctacatca tcaggcatgt ataccacttt taacttcaac tcttttatta tctctcttat
    28261 tgatacttta ttgctagttt ccattaattt gcttcctttc taaaaaaatt ataaacatta
    28321 tctgcaatgc ttttattcat tccatctact tctaaaagtt cttccatagt agcttttttt
    28381 atagcatcta catctttaaa gtgatttaat aatgatttct ttcttttttc acctattcct
    28441 tgtatatcat ctaagataga ttttgtcaaa gctttatttc ttagacttct atgatatgtt
    28501 attgcataat tatgaacttc ttcttgtata ctagcaataa atctatataa attagttgtt
    28561 ttgtctagct ctatttcttt ttctttgcat atcaatcctt tagttctatg cttatcatct
    28621 ttgtacattc cccaaagagg tatatctaca tcattaagtt ctagtacttt ttttactgca
    28681 ctaacttgtc cttttccacc atccaataat atcaagtcag gtaaatttcc atgttttaat
    28741 cttctatcta caatctcagc catagaatca taatcattag gtccaataac tgtctttatt
    28801 ttatatctcc tatattcctt cttatctttt ttagcattcg tgtacactac catagaacca
    28861 attgaatcta caccttgtat atttgatata tcataagctt ctattcttat tggcaatttt
    28921 tctaaattta atatttgctt taattcttcc aatgctccaa tacttttttc atattttctt
    28981 ttattcatat cagaaaattt ttcaagatac tctacagcat ttttcctaac catttcaact
    29041 aagcttttct tctctccctt ttgaggaact cttattgtta ctttttgccc ctttttagat
    29101 gacagccatt cttctaatat acaactatct tctatctcat cttctataat taactctttg
    29161 ggtatatact cttgttcatt ataaaattgt tttacaaatg aacttaatat agaagccctt
    29221 ggactatcca ttacaccttc taatataaaa tgttccctgc ctacaatttt accatttctt
    29281 atgaaaaata cctggacaca agcttcatta tgagctcttg ccattgcaac cacatcttga
    29341 tttaagtcac ttacagtagc atctattttt tgtttctgta tcatctcttc aaggctcttt
    29401 atcttatctc tatatacagc agcctcttca aatctaaagt tcatagaact ctcattcatt
    29461 ttttccttta aaagctccat caatttttct tctttgccag atagacacat tataatctct
    29521 tctatcattt ttccatactc ttctttagaa acatttcctg tacaaggtcc taaacattta
    29581 tttatgtgta gatttaaaca aggtcttgtc ttattcttta ttgctttatc tatatcaatt
    29641 ttacagcttc ttattggata agtactactt ataagctcca aagtatcatt cacagcagtt
    29701 atattggtat agggtccaaa atactttgcc ttatctttaa gaactcttct aacttttaaa
    29761 attcttggat agtcttcatt tgtagttact tttatatatg gataagtttt atcatctcta
    29821 agcactacat tatactttgg tctgtatctt tttattaaat tacattcaag tattaatgcc
    29881 tctaactctg aatccgttat aatatactca aacttatata tattcttaac catagatttt
    29941 actttagatg tatggttttt tgaagattga aaatactgcc ttaccctatt ttttagagat
    30001 atggcttttc ccacatatat tatatggtcg tatttgtctt tcattaaata tactccgggc
    30061 tcagaaggta atttttttaa atgctcttgt atatcaaaca tctcgccctc caaaacatta
    30121 aattttatac taaaattata ccctaaaata tgaaatttaa aaagttatac aatttccagt
    30181 actttagaca agttaaagtc catattccct tctacaattg atttaaaaaa caaatttaac
    30241 aatgaagagt aatatggact ttactttgta ttactattta caacttttat tttactaatt
    30301 tacttataaa agagcagttt agcgtatata tctagctcta aaatttttca caatttatat
    30361 gacttaagaa aaatattttt ttaagaattg acctgtataa gaaccatcaa ctttagatac
    30421 ttctcttggt gttccagttg caactatagt tccaccttta tctccacctt ctggtcctaa
    30481 atctattaca tagtcacaag tctttataac atctagatta tgctctatga ctataatcgt
    30541 atttcctgta tctgctagtc tttgcaatac ttggattagc ttgtctacat ctgccatatg
    30601 aagtccagta gtgggttcat ctaatatata caaagtcttt cctgttggtc ttttactaag
    30661 ttcagcagct aattttatac gttgtgcttc tcctcctgat aattgagttg aaggctgtcc
    30721 taacttaata tatgaaagtc ctacatccat taaagtttct aattttcttt ttatatttgg
    30781 tatattttcg aaaaactcaa cagcttcttc cacattcata tccaatatat cagatatagt
    30841 tttatctttg tattttactt gcaatgtttc tctattgtat ctttcacctt tacaaatttc
    30901 acaaggaaca tatacatctg gtaagaaatg catttctatt tttattattc catcaccctt
    30961 acaagcttca catctaccac cttttacatt aaaactaaat ctaccttttt tataacctct
    31021 tgctttggct tcattagtag ctgcaaaaat atctcttata aaatcaaata cacctgtata
    31081 agtagcagga tttgaacgag gtgttcttcc aataggactt tggtctatat ttataacttt
    31141 atctatactt tcaattcctt ttatctcttt atgcttacca gctctttgct tgagtctatt
    31201 cactttactt gcaacacctt tgtaaagtat atcgtttatt agtgtacttt ttccagaacc
    31261 tgaaacacct gttatgcatg taaacttacc taatgggaac tttacatcaa tatttttcag
    31321 attattttct gatgctttaa ctatttctat aaaacttcca tttccttctc tagtagtctc
    31381 aggaagcaat atttgttttt taccacttaa atactgacct gttattgagt tctcattttc
    31441 aattatttca tccaaagtac cctgtgcaac tatttcacca ccatgtatcc ctgcacctgg
    31501 accaatgtct ataacataat cagcttctct cattgtgtct tcatcatgct ctacaacgat
    31561 taaggtattt cctaagtctg ttagatgtct aagagtggct ataagcctat cattatctct
    31621 ttgatgaagt cctatacttg gctcatcaag tacatataag actccaacta atgcagaacc
    31681 tatttgagtc gctaatctta tacgttgtgc ttctcctcct gataaagttc cagcttttct
    31741 tgataaattt aaataatcga gacctacatc tcttaagaac atggctcttc cttttatctc
    31801 ttttataatc tcatgagcta taaatctctg tttttctgta agatttatgt tttccataaa
    31861 gtcgattaat tcatttacag ataagtttgt aacttccatt atattttttc catcaactaa
    31921 aacagataaa acttcctttt taagtctcat acctttacac tttggacatg gtctttcagc
    31981 catatattct tcaatcttat ctctcatgta atctgaatta gtttctctat atcttctctc
    32041 taagtttact attacacctt caaagtatga cttataattt ctagttcctc caaattttga
    32101 ctcataagta aactctacaa ttatattatc tttaccataa agtaattctt ccacaaaatc
    32161 atctggtaaa tctttaaatg gtgttgtcaa agatacatta aaatgctctg ctagactttt
    32221 tatcatttta ctgtaataag tgtcatcatt aacacctgtt gaaccccatg ctgccactgc
    32281 accttgtttt attgataaat ctttatttgg tactactaat tctgggtcaa cttctttact
    32341 ttccccaaga ccattacaaa catcacaggc accaaaagga gcattaaagg aaaacattct
    32401 tggagataat tcttctatac caatcccatg ttctgggcat gcaaatttag ttgaaaaaag
    32461 aatttcttct ccatctatta tttgggctat tacaagtcca tcagatagct taactgcagt
    32521 ctcaattgaa tctgctagtc ttgattctat tccatcttta attactattc tatctactac
    32581 aacttctata ttgtgttttt gatttttatt taggattata tcatcactaa cttcatagtt
    32641 ttcaccatta actctaactc ttacaaatcc ttctttttta atattatcaa aaactttttt
    32701 atgtgtacct ttttgtcctc taacaattgg tgaaagtatt tgtaatttcg ttctatctgg
    32761 aaaatctaac attttatcaa ctatttcttg aatagtcatc tgacttattt tttcaccaca
    32821 tgtaggacaa tacacatctc ctactctcgc aaataatagt cttaaatagt catatatttc
    32881 tgttactgtt ccaacagtag aacgtgggtt ttttgaagta gtcttctggt caattgagat
    32941 agctggcgat aaaccctcaa tatactctac atttggtttt tccatttgac ctaaaaattg
    33001 tctagcataa gcagatagac tttctacata ccttctttgc ccttctgcat atattgtatc
    33061 aaatgccaaa gaagacttcc cagaccctga caaacctgta aaaacaataa atttatctct
    33121 tggtaattct aagtctatat tttttaaatt atgctctttt gctcctctta ttataatttt
    33181 atcttccata atgttgtttt tacctttcta tttttccaat ttttcttcta attgtttcac
    33241 tttatctcta agttctgctg ctctttcaaa ttgtaagttt tgagcagctt ccatcatctc
    33301 tgattttaat ttatctatat tagctcttat ttcatcttta tcatctgtat cagatattcc
    33361 atatacaact tcttcctctg ccaccttagt agcttctata ctatctctta tatttttctg
    33421 tatagtttta ggagtgatat tgtgctcttc attatataaa ttttgtatat ctcttcttct
    33481 ctttgtttca tcaatagcat tttgcattga gtcagttatt ctatcagcat acataattac
    33541 tcttccattt tcatttctag ctgcacgtcc aatagtctgt attaaggctg tttcggaacg
    33601 taagaaacct tctttgtctg catctaaaat tgctataagt gaaacttctg gtatatccaa
    33661 tccttctcta agtaaattta ttcctacaag aacatcaaat ttccctaatc ttaaatctct
    33721 tattatttct gttctttcta atgtaactat atctgagtgt aaatatttaa cttttatccc
    33781 tatttcttta agataattag tcaagtcctc actcattttt ttagttaaag tggttataag
    33841 cactctttca tttttttcta tagttttagt tatttctcct accaaatcat ctatttgatt
    33901 atttataggt cttacttcta caattgggtc taaaagtcca gttggtctta ttatttgttc
    33961 tgctattgtc tctgagtgtt gaatttcata aggtcctggt gtagcactta caaacaacat
    34021 ctgatttata tttccttcaa attcttgaaa atttaatggt ctattgtcaa gtgcagaagg
    34081 taatctaaaa ccattctcta taagggatgt ttttctagac ctatctcctg catacatacc
    34141 tcttacttgt ggtatagtaa catgtgcttc atctacaatt atcaaatagt catctggaaa
    34201 gaagtccatt aaagtatatg gtctttctcc ttctgacctt cctgtaatat gtctagaata
    34261 gttctctatc ccctgacaaa atcctatttc ctttaacatt tctatatcat acttagttct
    34321 ttgttcgatt ctttgtgcct ctaatagcct atcattttct ttgaaaaatt taatttgttc
    34381 ttgaagctcg tcttctattt ctataattgc cttttcaatt ctttctggtg ttgttacata
    34441 gtgagatgct ggaaatataa caacatgatt tctagtacca acaatcttcc ctgttacata
    34501 atcaatctct gttattctat ctacctcatc accaaaaaat tctattctta ttgctttttc
    34561 atcattacta gctgggaata tttctagtat atctcctcta actctaaaag tacctcgtgt
    34621 aaaatttatg tcatttcttt cgtattgtat ctctattaac ctttttatta catcatctct
    34681 atctctttgc atacctggtc ttatagataa cataagttct ttatagtctt ttgggtcacc
    34741 aagtccatat atacaagata cagatgatat tattatagta tctcttctct ctaaaataga
    34801 tgctgtagca gaatgtcgaa gtttgtctat ttcatcattt atgcttgcat ctttctctat
    34861 ataagtgtca ctgtgagcta catatgcttc tggttgatag taatcatagt aacttacaaa
    34921 atattcaact gcattgtctg ggaaaaattc tttaaactca ctgtaaagtt gtgctgctaa
    34981 agtcttatta tgagccatta ttagggtagg tttcttaact tgttgaatga tatttgccat
    35041 tgtgaatgtt tttcctgaac ccgttactcc taataatgtt gagaatttct catttctatt
    35101 tatagaatcc actattgatt taatagcttc tggttggtca cctgtaggtt tgaagtctga
    35161 ttttatttta aaatccatag tatcactcct ttttcgaaca ttagttcctg acatgtatta
    35221 ttatatcata cctttataaa attgtttaag ttattttaaa tattttaccc atttaaattt
    35281 tacctaaatc taaaacataa aaaaagactt ctatctttgc aatagaagtc tataattcat
    35341 ggaaaataaa tactttattc aacttataca ataaaatcaa atgcttcttt ttcaacacca
    35401 tctagcttta aatcaaatat cttcaatata gttgctgcat gtgctctcag gtctccttcc
    35461 tctataacca caccactttt aatatttggt ccaaaagcta taaatggagg tctattacct
    35521 ttcgtaggta aatgaccatg tgtcgcaaca gcaaacttat aatcttttat atcagaatct
    35581 tttataatac ttccttctaa atcatttcca aatgaaatac cttcaatacc ttcaattacc
    35641 atttcaaaat caccttcaag accaaggtta gatgcttcct cttttgtaaa aattttctct
    35701 atgaataatt cttctttaaa ttcttcaaga agttctctta attctgttaa cctatctaag
    35761 ttatttacta ttatttgagc tgatattcca gaagaatcta cataggcatc aaaattctta
    35821 acttctccat tttcaacttt tataaatcca tttgatttaa gcaagacatt tggatttata
    35881 actttatcaa ctctaagatg accatggtcg ccaagtatta taaaatttgt atcttcgtat
    35941 gtacctgcat cctttgtagc ttgaataata tctcctagcc atttatcatt catttttaat
    36001 gctttatcta cttcttcatt aaataagcca taattgtgtc gtgtgtggtc caatgtagct
    36061 aaatgtatta acattaaatc tggcttatat tcctttatta tatctactga acagtttact
    36121 ccaaacaaat ccatattggg ttctagtttc caatcaatat aatgacaatg tttattgtaa
    36181 ataccatcca ttataacttt actggaactt cgttcaaacg tttctcttgg gtcatcttct
    36241 ctactaggag cccatatttc tgcaatattg taatctatat ttggatttgc tcccattaca
    36301 ggccataata cactagaggt agttagatta ttttcttttg ctacatctat aatagttttt
    36361 acttttatgt cctttgaata ccaataccaa tctttatttt ctttatttgg gtcaaatttt
    36421 tcattatgat atataccatg ttttttagga tatactcctg taaccatact agtatgacat
    36481 ggatatgtca gagtaggata aacacaattt atatttttca ctatggaaca atcttctagt
    36541 atagatttaa agtttggtag cttttttaca aattccaaat ctttttcaaa taaagaatcc
    36601 actgataata ctataacctt tgtcatttac attacctctt catttatata ttattgaata
    36661 cttctaatca acttacaagt ctatataatt atttatttgt tttatctatt actgcttgca
    36721 ttgcagaagt tcctttgctt actacatctt ttggatttgc atttaaatca gtaagtccct
    36781 ttttaatctc gtccattagc ttatcagttg cttccttata tccatttaac attggaagtg
    36841 caacagcata ctgtaattgg tcagttgcaa ccttatattg aggtttttct ttatataatg
    36901 tttgtaactt ttctgagttt gttgcatctt cagttgttgg taagtatcct gttgatgaac
    36961 taaactgaac gattttatct gtttgtgtca tccactctat aaattctgca cttgcttctt
    37021 tttctttatc tgttccacct tcaagcataa ctagattagc accacctgta ggagttgcat
    37081 atttttgatt ttttggtaag aatgctgttg atatttcaaa ctttccttct gtttgttgca
    37141 taagtcctgc taaatctgct gttgatgtaa atatcatacc tactttacca tttacaaaat
    37201 cttgttttgc tacatcccaa gcattataat cttctccagg aggcattttc attattccag
    37261 ctttcatcat atcttgccaa aacttaactg gagcttgtcc tgcttcatta ttaaatgcta
    37321 cttttttacc attagttatt tctcctccac tttgcattac catagcttca tagaaccaaa
    37381 tatctattgg tgtttcaaaa ccataagtgt cttctgattt atttgtcatc ttgctagcat
    37441 aatccttaag ctcttcccat gattttggac ctgaagggtc aagacctgct ttagttgcca
    37501 tatctttatt tatgtataat attggagtgc ttctattaaa tggcatacca acaaacttat
    37561 cctcataata tgaatttgtt aaaagaccag aaaaaaatct tttttgatat gttttatcaa
    37621 aatatttact catatctact aaagcacctg ctgatgcgaa tgtacctgtt tgagttattt
    37681 ctaataatgt tacatctggt tggtctttag atgttaaggc tgcttgtagt tttgttgcat
    37741 tttcatagta atctttctgc tttaacatat ttacttgtac atccttatgt tcactattaa
    37801 actggtcaac cattttttga gcagtttctc cattagttcc acctaaagga gcccagaaat
    37861 ctatctttgt aatattgcta tcagtctttt ctcctttaga tgcatttgaa tcattaggat
    37921 ttgaacaccc aactaaagaa cctaataata atgttattgc taatcctgtt gctaattttc
    37981 ttttaagctt cattttcatc ctccaaattt agtctttaat tttttatttt atccctgaat
    38041 acataaaagc ttcttttact tttttattcg caaatatata aacaagaatt attggtaaaa
    38101 ttagaattat atttcctgcc attattacat tccatgcagt aattccttct gaagatttaa
    38161 ggagtgcaac tcctattggt aaagttctta ttgcatctgt atttgtcatt actaagggcc
    38221 agaaataatt attccaatgg tatataaaat taaataatat aaatgttatt actgctggct
    38281 ttgccatagg aaccattatt ctaaacatta tttttaagtc agaagcatta tctagtttag
    38341 ctgcttcaat aatttcatct gaaacctgca taaaattctg tctaagaaga aatatcccaa
    38401 atgaagatgt cataaatgga attataagcg gaacatacgt atttataaat ccaaacttac
    38461 tcatcatcat atagactggt agaaaagtta cttgtggtgg aatcataagt cctacaagca
    38521 aaagtccaaa tagcactttt tcacctttaa atttaatctt agaaaatgca tacgctgctg
    38581 gaaccataac taaaaactgt ataattaaaa cacttcctgt tacaaataca ctgtttagca
    38641 aatatctaaa aaatggacct gaatgccatg ctgtactata attttctaac actatatcat
    38701 tgggtataat tgtaggtggt aattgcatca tttcttgcgg atttttaaag gatgtcgata
    38761 acatccatat aaaaggaaat ataaatattg cagataaaag taatagaatt aatatttcta
    38821 ttgattttga tgctattttt tttatatcaa ctttgtcttt tttgataggt tgtataatat
    38881 ccattttctc ctccttcttg ataagcctta taatatctat ttcttcttta ttgatagtga
    38941 actttttttg ataatagtct aaagttagca tatgtagcaa tcataactat agctaataaa
    39001 aacatactcg ctgcactcgc ttgccctatg tcataatata taaatccaga ctcatatata
    39061 taataagata gcatattggt tgaaccaact ggaccaccct gtgtcattat acttatctca
    39121 tcaaatactt gaaaagatgc agttgttgtt gttatcataa gaaaaaatat tgttggtgat
    39181 aatagtggca atgtcacttt aaagaaatta gttatcttgt ttgcattatc tagcttagat
    39241 gcttcatata tataatttgg tatggattta agacctgata atactattaa aatattgtat
    39301 ccaagagtct tccatattcc tattagaatt agtgacatta aagcagagct ttcacttgaa
    39361 agccactttg atgttggtaa atgtaatgta gatagtatcc agtttaaaaa gccatattgt
    39421 ggctccataa tccaaagcca caatacagct acagatacta gtgaaattac atgtggtgta
    39481 aagattatac tttgcgttaa attgtgtatt atagaatttt tatttagcca aacagcaagt
    39541 actatggcta atgatatgga tatagtcacc ataaaaaaag tataaattgc agtattcgtt
    39601 atagtttgta taaactctgg gttctttaaa agttctgtat aattgttcaa gcccacattc
    39661 tcaaaggttg aactaagaag actaccagat ttgaagctta gagatatcgt cttaattatt
    39721 ggatagacta taaacaataa agttcctata atagcaggtg ttatatataa atatgcttta
    39781 ttaaattgct ttttcataaa tatctccttt aattttagaa tacatatttt tacatatttt
    39841 taaatcttct attctttctc catttttatc aaaataatat aaatcttttt ctaatacact
    39901 tattcttaaa gttcttccaa tatcaaatat atcattttcc gttttgactc ttatctcact
    39961 attaagaaca gataaacaat atatagtttc atttcccaac acttctttag taatgacttt
    40021 tccaaataag ctaaatacat catcattttt tccatctata tttatacttt ctagtgttgc
    40081 tttttctggt ctaaatccaa acttaatatc attttttata tctagtatgt tcatacaagg
    40141 attccctata aattgagcta caaataaatt tttaggattt tgatatatct tctttggaga
    40201 acctatttgc attatctttc ctttgttcat tataactatc ttatctgcca tcgacattgc
    40261 ctcaacctgg tcatgagtta cataaacaaa tgtagatttt agtttttgat gtaactgtat
    40321 taattctgtt ctcatttgat ttcttaattt agcatctaaa tttgacaatg gttcatccat
    40381 taaaaatacc tttggatttt taaccatagc tctagcaagt gccactcttt gtctttgacc
    40441 tccagataag ttccctggtt tcacatttaa atattctctt aatcctacta catcaataac
    40501 ttctgttata agtttttctc ttttttcttt ttctactttt ttatttttaa gaccaaattc
    40561 tatattttct ctaacagtca tatgtggata taatgcataa ttttgaaaga ccatagctat
    40621 atccctttta ctagcatccg tttttgtaac gtcattttct tcaattttca acttacctga
    40681 agttatatct tcaagccctg caatcattct taatgtagta gatttgccac atccgcttgg
    40741 gcctaccaat actgtaaaag aaccatcttc tatagtaaga tttaaatttt ctataactgt
    40801 atttttttta ttataagatt ttgttacatt ttctagtctt atatctgcca tttttatcct
    40861 cccaaaatat ttaatttaat tctactattt acttatagat taataatact cttaatttgt
    40921 aaaggatata ttaagactaa gtgaatatat ggtaaactta ttaaaataaa ggttaaatta
    40981 taaacatttt ttgttaaata tactgaagtt aagatgatat acagcttata ttagctataa
    41041 ttttctatat tttcatattc atggtttgct atcacaatgt aatttaaatt tatgaagagt
    41101 catataaaac tatttattct tttatatgat ttggtttgaa aataaaaaag acctctatgg
    41161 gggatagagg tctttaagtg ggggagattg agtaatttat ttttgctata ttaagtttta
    41221 caattatatt atgtcccaaa tttacaaggt ctaaacataa aaaataaaac ttttttaatt
    41281 tttataaatt tacc
//
LOCUS       DAFNNB010000016        41002 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0016, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000016 DAFNNB010000000
VERSION     DAFNNB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 41002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41002
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0016"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            6..82
                     /locus_tag="L3M60_000763"
     tRNA            6..82
                     /locus_tag="L3M60_000763"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40..42,aa:Arg,seq:acg)
     gene            95..170
                     /locus_tag="L3M60_000764"
     tRNA            95..170
                     /locus_tag="L3M60_000764"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:128..130,aa:Val,seq:tac)
     gene            246..659
                     /locus_tag="L3M60_000765"
     CDS             246..659
                     /locus_tag="L3M60_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225459.1"
                     /translation="MYIVIGNEIVDSEELKVIIDKNSKFKVEKDLSKSTKREDVIAYQ
                     LSIDLNYLDSLISEQCNLASLSDEEKFDEYMTLSDELALNLEELMPKYTIINARAYKL
                     DEVDGVVKIILAVAYADLGHLKLSDVLMRISRQVD"
     gene            972..4403
                     /locus_tag="L3M60_000766"
     CDS             972..4403
                     /locus_tag="L3M60_000766"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HBN6225460.1"
                     /translation="MLKKFNKILVANRGEIAIRIFRACSELGIKSVGIYSKEDKYGLF
                     RTKADESYLIGEGKGPIDAYLDMDGIIDLAKRKKVDAIHPGYGFLAENAEFARKCEEN
                     GITFIGPSSKVMNMMGDKINSKKIAKEVNVQTIPGVEKAIRSTEEAKEVANKIGYPVM
                     IKASNGGGGRGMRIVHHEEDLELEYETACSESRKAFGEDIIFIEKYIADPKHIEVQIL
                     GDNYGNIVHLYERDCSVQRRHQKIIEYAPAFSLDDKVRKEICEDAVKLSKHVGYSNAG
                     TLEFLVDANGGHYFIEMNTRVQVEHTVTEMVTGIDIVQSQILIAQGYSLDSEEINIKS
                     QDDVEIRGYSIQCRITTEDPKNKFMPDTGKIQVYRTGSGFGIRLDGGNGFTGANISPH
                     YDSLLVKTISWDRTFQGAINKTIRSIKELRVRGVKTNVGFLVNVLNNPIFSNGKCSTK
                     FIDENPDLFEITESKDRGTKLLQFIGDVIVNDNACKEKPLFDALHDPRMDKDGSKAEG
                     SKILFDKLGKSAYIEKIKNDKKLLLTDTTMRDAHQSLLATRIRTYDLLKAAKPTEKYQ
                     KDLFSLEMWGGATYDVAYRFLKESPWRRLQKLREEIPSIMFQMLLRASNGVGYKNYPD
                     NVIEEFTKESARQGIDVFRIFDSLNWVENMKPSINTALETGKIVEATMCYTGDILDKT
                     KTKYNLEYYIKMAQELESLGADIIAIKDMSGLLKPYSAYTLVKELKKNIKAPIHLHTH
                     DTSGNGVATCLMASEAGVDIIDAALESMAGLTSQPSLNAIVEALKNTERDTGIDLFGY
                     DELGKYYKDLRKVYSKFESDLTNSCAEIYNFEIPGGQYTNLKPQADSLGLVNRFDEVK
                     EKYKEANEVVGDIIKVTPSSKVVGDLAIFMTKNKLDKDNIIEEGKNLSFPDSVVDYCK
                     GMIGQPEGGIPKDLQEVVLKGEEAITVRPGSLLPEEDFDEIAKYLNEKYDINANIRNV
                     ISYALYPKVYEDYIKHLQHYNDISKLESDVFFYGLNKNEECEVEIEEGKVLTIRLVEI
                     GEVKENGFRTIGFELNGMVREVEIKDKNFSGKINNVEKADMNDPLQIGASIPGKVIKI
                     MVKEEDEVKANQPLIVIEAMKMETIIVAKTDGVIKSIKVKEDDMVEDKQLLMIMK"
     gene            4645..6573
                     /locus_tag="L3M60_000767"
     CDS             4645..6573
                     /locus_tag="L3M60_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBN6225461.1"
                     /translation="MKVYDSKMLRNVAVLGHSGCGKTNLIETIAYTANTNKIPKLTDK
                     VNMTYSMGLIPIEYNDYKFNLLDTPGYFDFSGDVVSSLRASDAAIIVIDATAPIQVGT
                     EKSLELTESIPKIMFINKIDNEKARYKDAIAMLREKYNNKIVPMISPIYKDKNFVKLH
                     NVFENIDDLEGEFKEQAMSVKEALMELIAETDDQILDKYFNGEELTTEEIQKGIIIGI
                     QRGDIIPVICGSTINNIGTKEILDTISNYLEPIFTEESKPFRGLVFKTMVDPFVGKMS
                     YIKITEGVLSKDKDVFNINKNVKEKIANIYTLKNSELVEVEKAKAGDIVVVTKINSLK
                     TGDTISIDKDAEALENIDFPKPQIYYAVTPKNKGDEEKVASVLNRLVEEDPTLHSYRN
                     TETKQALLGGQGELHIKTIKNKMKDKFGVDVELNDLKVPYRETIKGKSDVQGKHKKQS
                     GGHGQYGDVKIRFERCESDFEFAEEIFGGSVPKQYIPAVEKGIKDSMQKGILAGYPVT
                     NIKATLYDGSYHDVDSSEMAFKMAASVAFKKGMEEAHPILLEPIMKLKITVPEEYMGD
                     VMGDINKRRGKIFGMEPDDKGKQIIFAEAPQAETFKYAIDLRAMTQGRGYFEMELERY
                     GEVPSQFAEKIIGLATAK"
     gene            7030..7494
                     /locus_tag="L3M60_000768"
     CDS             7030..7494
                     /locus_tag="L3M60_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HBN6225462.1"
                     /translation="MATMTDIIEKFIKDLMEEDNSIQIQRNELANLFSCAPSQINYVL
                     TTRFTIDRGYYIESKKGGGGYVQIEKIRKSKDGHIRELLNEKIGNQISYKKAKELLEG
                     LKESDLINERELKLILYAIDDKSLCMPIYELKEKVRSNILKNIIIGLFSIEE"
     gene            7501..7995
                     /locus_tag="L3M60_000769"
     CDS             7501..7995
                     /locus_tag="L3M60_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HBN6225463.1"
                     /translation="MLCQKCNKNKASVYYNKIVNGEKTEMYLCSECAKENTEMNFNLD
                     MPFSMMDIFSNLGFQPKKELEEKLVCPKCNTTYSEFKNNGRFGCSECYNAFSSQVNPM
                     LQNIHGHIEHTGKAPKKSFYKISVENEIKELKEDLDRAIKNEEYELAAQFRDKIKYLK
                     GSID"
     gene            8011..9036
                     /locus_tag="L3M60_000770"
     CDS             8011..9036
                     /locus_tag="L3M60_000770"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HBN6225464.1"
                     /translation="MKENIVMKSRVRLARNLNNYSFPNKLDKECAMEIIEKVKNAFIN
                     SNLEQKEEFDFYKIEDLDQSKKMLMVEEHIISPDLAESDKSAVIVKKDKTVSIMINEE
                     DHIRIQTMCDDLNLEYAYSVANEIDDLLESSLEYAFSTKLGYLTSCPTNTGTGMRASV
                     MMHLPALSQLGYMDELYKISSQIGIAIRGIYGERTEALGNIYQISNQLTLGRTESNII
                     ENVSGLTKDAISKEIKAREILQKKLGIKLEDKIFRSIGTLENSRIMSSAEAMSHLSNI
                     KMGIEMNYIDKLDLRAIEQLMIGIQPAHQSIMYKSDDVENRDINRATYIRETLEKLRG
                     GMNCELQ"
     gene            9023..11470
                     /locus_tag="L3M60_000771"
     CDS             9023..11470
                     /locus_tag="L3M60_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HBN6225465.1"
                     /translation="MNFNRFTQRAKKAIDLAFESAKGLGHNIVGSEHILLGLLREEEG
                     IAAKVLSKVGFTEAYLEGKIVDMEGKGEEISEDIVLSPRSKQILELSGMFANKLKTNY
                     IGTEHILLAIIQEGEGIANKILNYAGVNDRTLAQLTIDMMGISDKNQYKAENSYTGNQ
                     NQAESKVLDKYGRNLTLYAKQNKIDPVIGREKEIQRVIQILSRRTKNNPVLIGDPGVG
                     KTAIAEGLATNIALGNVPETLKNKTLYSLEMGSLLAGAKYRGEFEERIKEVVDEVVKN
                     GNIILFIDEMHTIIGAGSTGEGSIDASNILKPALARGEIQVIGATTIDEYRKHVEKDS
                     ALERRFQPVMVDEPTKEDSIKILEGLRDKYEAHHKVKITDDAIKAAVELSTRYISDRY
                     LPDKAIDLIDEAASKVRLKENTPPSEIKKLELEIENIDKEKEEAVRCQDFEKAAKIRD
                     EQGLLKKQLEDVRERWSKSSKHSDLVDGEVIAEVVGLWTGIPVNKILEEEADRLLRLE
                     EILHNRVIGQEQAVKSISKAIRRSRAGLKDPNRPIGSFLFLGPTGVGKTELSKALAEV
                     QFGDENQIIRIDMSEYMEKHAVSRMIGSPPGYVGHDEGGQLTEKVRRNPYSVILFDEI
                     EKAHPDVFNILLQILDDGRLTDSKGRTVDFKNTIVIMTSNVGASTIGRQKTLGFSIAK
                     GDEEEKSQYEKMKENIMGELKQRFRPEFLNRIDDIIVFHSLNENHISKIVLLMAAKLQ
                     ERLKEMDIKLEMSDEAVKLISKSGFDLEYGARPLKRALQKELEDELSEAILKGNVKKG
                     SNVVAKVKDEKIVFETK"
     gene            11650..13023
                     /gene="radA"
                     /locus_tag="L3M60_000772"
     CDS             11650..13023
                     /gene="radA"
                     /locus_tag="L3M60_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBN6225466.1"
                     /translation="MAKIKTKYVCQSCGYETAKWLGKCPECTKWNTFVEEIDQKSTKK
                     EVFIIDKSSSKPVTINSIESKEEERFTTDINELDRVLGGGIVKGSLVLVGGDPGIGKS
                     TLLIQVSSNVANLGKTVLYITGEESESQIKMRAKRLGINSENLYIFAENNLSIIESYL
                     EVVNPELIILDSIQTVFSPEISSAPGTVSQIKEGTSKFMKISKKMGISTFIVGHVTKE
                     GSLAGPKLLEHMVDTVLYFEGERYNTYRLVRAVKNRFGSTNELGVFEMRDLGLVELDN
                     PSKILISEKPKDVAGSVIISTVEGTRPMLLELQALVSPTSFGIPKRTSTGVDYNRVGM
                     LLAVLEKRVGLQIQNQDVYINIVGGIKINEPSIDLGIAISVASSFRNIPIDEDIAVTG
                     EVGLTGEVRAVSFIEKRIAECKKLGFKKIVVPRSNYDVIKDTKGIEIWPVDNLRQAIN
                     IVLGRNQ"
     gene            13026..14096
                     /gene="disA"
                     /locus_tag="L3M60_000773"
     CDS             13026..14096
                     /gene="disA"
                     /locus_tag="L3M60_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning protein DisA"
                     /protein_id="HBN6225467.1"
                     /translation="MENFLDNKNMLYALKMISPGTPLRLGLNNVLRAKTGGLIVIATN
                     EDVMKIVDGGFAINAEYSPSYLYELAKMDGAIVLSGDVKKILFANAQLIPDYFIETSE
                     TGTRHRTAERVAKQTGAIVIGISQRRNVITVYRGNEKYVVEDISKIFTKANQAIQTLE
                     KYKTVLDQAITNLNALEFNDLVTIYDVALVMQKMEMVMRVTSIIEKYVIELGDEGTLV
                     SMQLEELMGTTRIDQKLIFKDYNKENTEIKELMKKVKNLNSEELIELVNMAKLLGYSG
                     FSESMDMPIKTRGYRILSKIHRLPTAIIENLVNYFENFQQILDASIEELDEVEGIGEI
                     RATYIKNGLIKMKQLVLLDRHI"
     gene            14185..15279
                     /locus_tag="L3M60_000774"
     CDS             14185..15279
                     /locus_tag="L3M60_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HBN6225468.1"
                     /translation="MIRKVTRILFILLGFTIGITTYLTLMKDFEILTFGKETYGYIAA
                     VVAGIIIAILGYFIEPWVVNKVKEIAKIVDKELSKYPQTDILLGSMGLIVGFVIAYLL
                     SGLVNRIPIVGGILSLLLYLFLGYLGMKVALKSKNDLFNIGKLGRLANPIKDKDKDKE
                     NKKDIKAIPPKVLDTSVIIDGRIADICKTGFIEGKLIIPAFVLEELRHIADSSDDLKR
                     VRGRRGLDILNIIQKELNIEVEISERDFDDIAEVDSKLLKLAQVLNGKVVTNDYNLNK
                     VAQFQGVEVLNINELANAIKPVAIPGEDMVVQVVKEGKEAQQGVAYLDDGTMIVVDGG
                     RKYMNETIKVLVTSVLQTPAGRMIFAKPKN"
     gene            15408..17585
                     /locus_tag="L3M60_000775"
     CDS             15408..17585
                     /locus_tag="L3M60_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225469.1"
                     /translation="MRKKIISFLATFIIILTSASQYIFADDISTRGKVIFIDMNRTSM
                     SNMLRIKSLREELDNRGYIGLMNIRGDKGSDDRRSYASMGAGGRANVANEEDINFESS
                     SKDRNIVFESATGKSAKGINNLTINKSVNENLNFGEYGSVLGSLGQSLSDNGLKASVL
                     GNSDIIENGQLIKNRNLCLTAMDEYGRIPNGNVDTINKKDLSMPYGISTDYDKLIVET
                     KEAYKNNDVVFVELGDTYRLDLYKPNLNEKTYENMKNKIEKNIDVYLKNIFSMVGEND
                     TIYIASAFPSDLDYKNKRRLSPIVKLNGVGKGLLSSSTTRREGIVTNLDVGVEILDNF
                     NIKNQNMVGRKYELINRDDNKEFLMDEYQKIVSISSIRSTILNGFVSIVFLSWIVAMI
                     AILFRKHISKKYKETTFFILKELLKIGIVMPLSFMITPIMNFKTPLAISLGIIIITLT
                     IYLISKVLIKNDLKNMLFFTGLTIVIMVIDAGFGSYLMKSNVMSYDCIIGARYYGVGN
                     EYQGVAIGSAIFTFAILLTYKNIPKWSVIVFSLLILITSASPIMGANVGSAISECVAF
                     LLFILLIYNVKIDFKKIVLLGIAVLFVLGAFVAIDMILGSNSHLGMFVKEIYFNGPGE
                     IIQTFSRKIEMNLKLAQTSAWVNILLTGIGVILALMINQIRYFKQLMDEYPIVFKGFI
                     ASIAGCLVTLLVNDSGIVSSATAFIYIIVPMITLSVNLTALKE"
     gene            complement(17764..18615)
                     /locus_tag="L3M60_000776"
     CDS             complement(17764..18615)
                     /locus_tag="L3M60_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6225470.1"
                     /translation="MNRNSFSHEIIEPIDNLDVNFKLFDDSGSYVANHWHNSLEIIYI
                     TSGDLQINMEGYTYNLKANECMFINSGIIHSTRCTYHNTSILLQVPMSFLNKYIPDFK
                     NCYFDFKVNANDNNYKRNSSKVKLILENMREIKLSSPPAANLQFTSLLFELLFELYTN
                     FKISVGNKNMKKTVLDLSKFEPVLEYTNINYKSPISINKIAKVAHLQPEYFCRKFKQY
                     MGQTYLEYLNDVRISHIYKDLINTNDTLCSILETHGFTNTKLFYRIFKEKFKCTPKHI
                     RKNLPKI"
     gene            18832..19998
                     /locus_tag="L3M60_000777"
     CDS             18832..19998
                     /locus_tag="L3M60_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HBN6225471.1"
                     /translation="MYIKGVNLGGWLVLEKWMTPSLFEGTEAEDEYYLPRQLSRDVYE
                     SKIKTHRSEYITERDFAIIKSMGFNSVRIPVPYFIFGDCEPFIGCVKELDKAFYWADK
                     YGLSILIDLHTVPGSQNGFDNGGISGVCSWSQNPEYVEFTLNVLERLAKRYGMRRELY
                     GIQILNEPITERMWNIMNVPNRFKAVDKDMARRSKPNSLEFLRDFYIKAYKVMRPYMS
                     EENVIVFHDAFELKSWKDFMKEEEFKNVVLDTHQYLMLAEADGCEQSIDSYLKYIRDN
                     YAKDILQMQKYFPVICGEWSLFNSYACGIDTAGGQSPLNGIESNIDKLSKDERRELYR
                     KIAKAQIDAWRNGSGHYYWNYKLLLDTVNEEGWIGWDSWDLGKCVAQEWYPIEY"
     gene            20020..21666
                     /locus_tag="L3M60_000778"
     CDS             20020..21666
                     /locus_tag="L3M60_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HBN6225472.1"
                     /translation="MIKNPILPGFNPDPCICRKGDDYYLVVSSFEWFPGIPVYHSKDL
                     KNWELYTHILTDETKIDLKKLPSSKGIWAPCLTYCEEEDLFYIVFGIMNSMNARYFDV
                     DNYLITSKDIKGEWSEPVYLHSSGFDASIFHDDNGKKWISSLDWETREGYEKPGVICL
                     VEYCIEKKEIVGYPKRIWSGGTDRGCIEAPHITKRGDYYYIMCAEGGTGYGHGVTMGR
                     SKNIWGPYEKDLMNPIVTSIPGDFYERHDPDHLKPKYYNPESKLQKSGHGSYIETPSG
                     EVYLVHLTSRPFVPELRCTLGRETAIQKMKWTEDNWLRMEDGSNLAKEYVSESKLEEH
                     LVSSIPSFDDFDSNKLGLQYYAPRISPLSFADVNSRTGYVRIRGQESRTSLNRVSILA
                     RKLTSVYARITTKMEFYPEVHQHSAGLIMYYDNMNYINLRKYYSETLGQSALSIIHLE
                     NGEKTEFLNTRIPIKDIPIYLRLYIKGRKSYFEWSYDGEKYQRIGETFDTTKFSDEYC
                     KYGEFTGTFIGLTCADRVKHKHYADFDFFEYIADESKDVD"
     gene            21764..23137
                     /locus_tag="L3M60_000779"
     CDS             21764..23137
                     /locus_tag="L3M60_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225473.1"
                     /translation="MNKEVNVKATTIKYEKISFFERVSYGCGDLGCNIIYSAMSAFLL
                     FYYTNYADVSAAAVGSIMLISRILDGFSDLTMGIIVDRTKSKYGKARPWILRMAIPFA
                     ISAILLFSVPSSLGVTSKLIYIFITYNLVSTVIYTAINVPYATLNSLITQDQYERGVL
                     SIFRMILATCGTLIINGLTLPLVEHFGNNLSAWTKTFFIFGIASIIVFFITFTGTKER
                     VKAVKQNKNEVIPFKIGIKSLFRNKYWIQITLCLVCIFIVFAINGGSSVYYAKFILGD
                     EKLFAPINMVSNVSQIIAMFMVAPFIKKFGKRNVLIVGSIILISSNVMFIIAGQNYIG
                     VISASAIKGIGSAGIAATMFAIVSDTIEYGEWKTGYRTEGLINSASSFGFKVGNGLGS
                     AILGGVLSIGGYVGTSATQSNSAIVSIKACFIYLPIFITILQIIIMFFYKLDKEYSTI
                     LNELNTK"
     gene            complement(23382..24560)
                     /locus_tag="L3M60_000780"
     CDS             complement(23382..24560)
                     /locus_tag="L3M60_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225474.1"
                     /translation="MTINHTRLLQTFVELGINSYRQRGTDILKKLYFRFIAYFTTGLF
                     IFLVYFITLFILLNQLIYPEDSHKFLYVIDSSIIYFPSTLIFTFCFITGGILFCLFLV
                     RPLYLIISSISNLSRGNYTISEKAFTKNGKLKWYNFMYREVIINIKDLGNKLQETEIE
                     REKLEVAKTDWLASVSHDLKTPLSYITGYSSLMLNKNHSFSEEEMLTFLNNIYTKGIY
                     IEKLIDELSLTFFIDSHNKIHLKHTQVEIVSFLRNLIADITNNPKSEKHIFEFHTDLQ
                     HLNLVIDETLMYRAIYNLLINCVEHNPSGTEIDVVLTQKSDEIYININDNGIGINPET
                     LNDIFAKYYSSKNQNTQNKGLGLFIVKQIIDAHEGNISVSSIMGKGTSFQIMLKQHKP
                     "
     gene            complement(24484..25218)
                     /locus_tag="L3M60_000781"
     CDS             complement(24484..25218)
                     /locus_tag="L3M60_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225475.1"
                     /translation="MNDKFKNSERILIVDDEEGLLTLLKITLNKEQYNNITCVSTGAK
                     ALECIENNQYDLIILDVMLPDYSGFDLCTNFRKKTIAPIIFLTARASDYDKLSGLAIG
                     GDDYITKPFNTMELVARIKAIFRRQQLERSVLCNHSENILVYNYGYIVLDMAHALLTV
                     SGKNVECTAKELELLEFFCQNPNRVFTTSHLYDKIWGLQGYGDEKTVTIHISKLRKKI
                     LDDNKPYKVITNLRGIGYKFIPPKGN"
     gene            25386..26837
                     /locus_tag="L3M60_000782"
     CDS             25386..26837
                     /locus_tag="L3M60_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HBN6225476.1"
                     /translation="MIKSLKSRGISILLCGALIVGVMTGCTSKATSPKSSTPKSTPKT
                     VEKVTRANCEKQLKEVKAKYKKNPNDEAIRLEYAQILFKLGNITEAQEILNPLLNSKK
                     PSPDAIFLSARIEYLNGNYTQAEKLYDNLIEQYPDKFKSKAEDELALTYYQTNQYQKA
                     DKLSSVKTNNALLEMMKAFKDKHPNKIDWNGKKETVIPFVTGDPLPIIPMEINGKRIN
                     TIIDNGAPMFVVDEKIAEEIGINLASKEENTKFAGGNSIGINFGQANSLTIGDVNMEN
                     IPVMLGPFDGFEKLFKDYGNVHGIMGTTVLKEFVPTMDYPSGQLILRPRNEVGRDNLN
                     KMLTNDKILEEVPFTLASTHFMFGKGSINQKVGLNFFVDSGLGDEKGAGIVLTEEIMN
                     SLKIPMPELKKAEEGKGGMAGSDYKEGYFNLSSYGLGDLQLKNVLGYYFTGDTLDYDE
                     GIGFVRDGLISHHYLKNYKWSIDFDSMKMTFSK"
     gene            27041..27994
                     /locus_tag="L3M60_000783"
     CDS             27041..27994
                     /locus_tag="L3M60_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HBN6225477.1"
                     /translation="MKKIGDLRLMMKCCSLYYEDNLNQQEIANQLGISRPTISRILKE
                     AFEQGIVKIQIVDVLKNDYQKIERSLERKYKLKEVIVVDDKQDSLTQKQELARAVSEY
                     LTRVVKENDIIGVSIGTTLKEIPRYVEKSNCKNVTFIPLLGGIGDNEIDIHANQIAVS
                     LARAFGGDFKLLHAPAVMSDLSTKEKLCKDEKIKEVLDLIDKTTIAIVGIGNPMSLNS
                     TIMASGYMNEADIEDLKKYNSIGAICLQAFDKEGKTSILEFNQRVLGVKLEDLKKIKR
                     TIGVASGDEKIEAIKASLKAKFINSLAINHSLALKLLEDCD"
     gene            28032..28994
                     /locus_tag="L3M60_000784"
     CDS             28032..28994
                     /locus_tag="L3M60_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HBN6225478.1"
                     /translation="MSISKETLLEMYKRMNQARKFEEKVSWFFARGMVHGTTHLSVGQ
                     EASSVAAVMALEKGDLVSLTHRGHSQFIGMGIDLNKMMAELMGKETGFCKGKGGSMHI
                     ADIESGNLGANGVVGGGLTIAPGAALTQQYKKTGKIVLCSFGDGASNEGTFHEGINLS
                     SIWKLPIIFYCENNLYGMSTSIKRHMNIESIATRAASYGIEGISIDGYNPIEVYETVQ
                     KAAEKCRRGEGPVLIESRTYRWLGHSKSDANVYRTKEEIESWKAKDPIEFLRNYLIEN
                     NLSNEDELDKIQEFAKQSIEDAVEFAQNSPNPKIESLLEDVYAD"
     gene            29019..30005
                     /locus_tag="L3M60_000785"
     CDS             29019..30005
                     /locus_tag="L3M60_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HBN6225479.1"
                     /translation="MSTRELTYAQAIKEAMSEEMRRDENVIFMGEDIGIYGGAFGVSV
                     GMIDEFGPERVRDTPISEAAIAGAAAGAAATGLRPIMEVMFMDFVTISMDAIVNQAAK
                     MRYMFGGKAQVPMVVRCPGGSGTGSAEQHSQSLEAWFCHVPGVKVVAPSTPADAKGLL
                     KAAIRDNNPVIFVENKLLYRKKGVVPEDDYVIEIGKADIKKEGTDVTVITYGRMLQSV
                     EEAAENLSKENINVEIIDLRTLYPLDKETIVKSVCKTGRVLICHEAAKTGGLGGEISA
                     LITESESFDYLDAPVKRICGKDVPIPYNPELEKAVVPRVDEIEEAIKSLIVR"
     gene            30018..31064
                     /locus_tag="L3M60_000786"
     CDS             30018..31064
                     /locus_tag="L3M60_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HBN6225480.1"
                     /translation="MVANKVKATPAARNQARKDNIKLDRLIGSGENGRIHLVDVLNYL
                     KDNKANTTPLARRIAEDLNIDLETIVGTGYNGKIRKCDVEKLTEKETIVSTNTSKFSE
                     KKETKIENENSSMFNTVEGVFEKPNPMRATVARRMSESYFSAPVFTFNIEVDATELKA
                     LRVKLIDTVKESTGVKLTMTDLIVMAVSRILPNHQALNSAWTDEGIFRYKDINIAIAV
                     GLDEGLYVPVVKSVNKKSLKEIAKESKELAEKVKTGKLMPADQEGNTFTISNVGMYGI
                     TTFTPIINMPSSAILGVGATQDKFVPVNGEAKIKPIMNLSLTSDHRVIDGTVAAKFLK
                     DLKELLENPLSMLV"
     gene            31231..32961
                     /gene="lpdA"
                     /locus_tag="L3M60_000787"
     CDS             31231..32961
                     /gene="lpdA"
                     /locus_tag="L3M60_000787"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBN6225481.1"
                     /translation="MSVEVIMPKAGVAMEEGTIVSWLKQEGEEVKIGEPILEITTDKV
                     NMEIESEGEGTLAAIIHKEEGEVLPVFTVIGVIAEKGENQEEVKAKYLSGNVSKEDIV
                     EENQNIEAKEEKINKKECNHDYDVVVIGGGPGGYLSALKAALLGGRVALVEENILGGT
                     CLNRGCIPTKTYIKTAEILEEIDQLSKRGVKVTVDKEQDIKKAIKYKNRVVKKLTAGV
                     GGLLKSRDVEVFNLKASVKEEHKVILSDGKVLDTENIIIATGSKVRILPIKGIESNLI
                     ITSTEALDLETVPEELVIIGGGVIGCEFAEIFNSRGSKVTIVEMEDRVIPKMDKDLSE
                     SLKYSLGKKGINVLTKKKVSEFKEEGNNILVCIEDEEPIKADLCLYAIGREANLSGIE
                     DLDIKIDKGSIVVNSKMETSIPSIYAVGDVTGGVMLAHAAFKMGEVAASNALGMNKEV
                     DLGALPSCVYTIPEVASVGITEEDARKKYNVKVGKFNFAGNGRALASGQEQGYVKVVA
                     DAKYGEILGIHMFGCGVAELINHAASFKALEIPADEASELIFGHPCTSEALMEALADV
                     NGECLHLPKK"
     gene            33367..36315
                     /locus_tag="L3M60_000788"
     CDS             33367..36315
                     /locus_tag="L3M60_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6225482.1"
                     /translation="MRQRIKNLIENEDKKNPLTDEAISSMLNIRREDVTFLRNELKIE
                     DSRQRRKVVLIKAIKDILKEEKTINKNDITRRLNSIGFTVSRFTVIQYLKELQDSDNL
                     KLYKESDDKKEEINNNRENTDIAFDKLIGSSGSLSTIIKLAKAAILYPPHGLHTIIMG
                     PTGVGKSELAECMYKFALESSRFPKNAPFIVFNAADYSENPQLLLSQLFGHVKGAFTG
                     ADESKEGLVSKADGGILFIDEIHRLPHEGQEILFQLIDKGMVRKLGETKLTNKIDVMI
                     ISATSEPIDSHLLNTFKRRIPVTIEIPELVARPLNERFDIINNFFLIEAQRMGANIHI
                     KSDVLKALMLYDCIGNVGQLRSDIQVACARGLLNQLTNKQKEVNISISDVPGYVKQGL
                     IKIRNCRGKIENYVDGDLIIDSNLQGEIEKKNEDIYTFPDEIYKLTERRHIDLLEQGL
                     DFDVINRIVGGELESCIQKYIKQVKKVSISNNLPDISPIVGKYIVELTYEVIKIASQY
                     LNSLEPNLHLCLAVHLKAAFERLKEGKVISNAHLKEIIGNYPLEYSVAEKVAQYLKER
                     YNINLPKEEIGIIAIYFKMSSRRNISEQKKIGVLLMAHGRVASSVCEVTNKLLGINHA
                     RYLDMPFEKKPEEMIEEAITIIKDIDEGRGVIILVDMGSLSYFGDIISGKTGIDIRTI
                     SRFDTLLSIEVIRRAVLPDLTIDEIVDEVKGIEPQVKEKVSKYNNTKVNFRKPIILTL
                     CITGLGGACKIKKILEENIKSITEKVDIEPIGMIEYPSLDLEIKQISNDKNVIAIVGT
                     IDPQIANIPFFSLVDINDKVKLNQINNLIDTNQISETNKKYELSDLIHKEDIFVDLQV
                     KSKEEVLKYMSKKLREKGYVTKNFYKKVVERDNLFPTELSDEIAIPHTDGIDVIRPAI
                     SIAILKNPIVWSKNKVKIILLLAVDQKCFNPLSTLLSFIETDEFKQIKNIKDRDFIRE
                     MILDGVNENN"
     gene            36293..36757
                     /locus_tag="L3M60_000789"
     CDS             36293..36757
                     /locus_tag="L3M60_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225483.1"
                     /translation="MESMKIISSNLIFKNIEVSNNEDALKFLGQRLFDEQYVKASYIQ
                     AVIAREKKYATGLPTEIYGVAIPHTDIVHVNEPGIAIGILNKPVKFIMMGTDDTEIDV
                     KVVFMLAVKEPQEQLQLLEKLMTIFQDKNILNNIVDLSEESVSDLLNSKLKN"
     gene            36836..37138
                     /locus_tag="L3M60_000790"
     CDS             36836..37138
                     /locus_tag="L3M60_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225484.1"
                     /translation="MGVTNVTKKILVACGTGVCTSTIAINKLKKALQDIGKLDMVNIT
                     QCKVVEVASKAPDYDLIICTTQVSSSIKTPVINGLPFLTGVGMDKLIKDVLEKLEL"
     gene            37200..38564
                     /locus_tag="L3M60_000791"
     CDS             37200..38564
                     /locus_tag="L3M60_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421211.1"
                     /note="with GatAB forms a phosphoenolpyruvate-dependent
                     sugar phosphotransferase transporter for galactitol;
                     subunit IIC forms the translocation channel and contains
                     the substrate binding site; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBN6225485.1"
                     /translation="MEGLYMEILNFIVGLGAQVMMPIIICIFGLILGTKLGKSLRAGL
                     TVGVGFIGLNTIIALLTDNLGPATQQMVKNLGLSLSIIDVGWPAASAIAFASTVGALI
                     IPIGLVVNIIMLITNTTQTVDVDIWDYWHFAFTGALVAGATQSVMWGVFAAVANMIIV
                     LVMADLTAPGIEEYLGMPGISLPHGFTQAFVPIAIVVNKLLDLIPGINKIEINADTLQ
                     KKFGLFGEPLIMGSVIGVIIGVAAKYDIKGILQLGVTMGAVLILIPKMAALLMEGLLP
                     VSESAQEFIEKRFKNRGKIYIGLDSAVGIGHPVTLSVALVLVPLTILIAAILPGNKVL
                     PFADLAVIPFALVLIVPITKGNVFRTLIIGIIIITSGLLIATNLAPLFTQMALNASFK
                     MPEGATMISSICDGANPLSWVFVKVMNYKVIGGVVFGVIALGMAIYNRNRIINENKKL
                     FIEE"
     gene            38599..39072
                     /locus_tag="L3M60_000792"
     CDS             38599..39072
                     /locus_tag="L3M60_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225486.1"
                     /translation="MKKYKYDSKRYLLSVTIPGFFMILILIYALFNNYINFNLNIYSF
                     LIIVCTYGIFNTFISSSNPKKIIIDNKCIHFSSFMTTHKYEIKDIEKIRIREFYNKKI
                     YLKINEGNLFKGRYWIRTNMFNNSIELYSYLIELEECLYPDSLKFRNKRFENKNI"
     gene            39235..39885
                     /locus_tag="L3M60_000793"
     CDS             39235..39885
                     /locus_tag="L3M60_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBN6225487.1"
                     /translation="MLLDKLRKEVLQASLDLLNYNLVTLTGGNVSGRDEQTGYIAITP
                     SGMDYRNLIPSDIVIVDVDGNVIDGKWKASVDLPDHLYIYKHREDINSIIHTHSTYSS
                     CFAILNEPIECASTTLANEVGGSVPVAKFSPPTSNKMGKCVIEAIGDKRACLLANHGV
                     IAVGPSVGHALTAAVMLEDSAKVYYLAKSIGTPVLLPDEEIQRARDVFFNVYGQDK"
     gene            40061..40993
                     /locus_tag="L3M60_000794"
     CDS             40061..40993
                     /locus_tag="L3M60_000794"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBN6225488.1"
                     /translation="MYYLLNKSTNPYFNLALEEYLFLNDKYNDDIIIIWRNEESIFIG
                     KNQNPYQEVHHDVIEKGEIPILRRISGGGTVYHDLGNINMSFIQKDRQLHEIDFLEHT
                     KFMQKMLLTLGLGVSITERKDLFLNGKKISGSAQSIKRKNSLYHGTLLYDSDLNKLTK
                     YLNSDKSTESNATKSVSSKVTNIKFLLEKNISYFLDYCVEYLKSNIKNIKELELDEED
                     IKNVYNLVEDKYKKIEWTYGKTPKFQVVLPFNNKSKINIHVNRWKVSKFFISYDDNVI
                     NMDKLLDLNFFKEEIKESILENYPHYIDLINLIF"
BASE COUNT        16592 a         4417 c         7501 g        12492 t
ORIGIN      
        1 aaaatgcgct catagctcaa ctggatagag tgtctgacta cgaatcagaa ggttaggggt
       61 tcgaatccct ttgggcgcac catataaata gcgagggatt atagctcagc tgggagagca
      121 cctgccttac aagcaggggg tcacaggttc gagccctgtt aatcccacca gtgcaaacct
      181 ctagtaattt tactaggggt tttatttttg attgaaaaag tttttatatt taggaggtaa
      241 gaaacttgta tatagtaata ggaaatgaaa tagttgatag tgaagaactt aaagtaatta
      301 tagataaaaa tagtaaattt aaagtagaaa aggatttaag taaaagcaca aaaagagaag
      361 atgtaatagc atatcagtta agtatagatt taaattattt agattctctt ataagtgagc
      421 aatgtaattt agctagttta tctgatgaag agaaatttga tgaatatatg actttgtctg
      481 atgaacttgc actgaattta gaagaactta tgccaaaata tacaataata aatgctagag
      541 catataaatt agatgaggta gatggagttg taaaaataat cttggctgta gcatatgcag
      601 atttaggaca cttaaaactt tctgatgtat taatgaggat atctagacaa gtagattagg
      661 atacgaatga atatgaacta tattaaaaaa atgtataaaa tcttacgtaa agatgaaaat
      721 aattatgtaa acacagaaaa aaacagtata aaatcaagtc aattgtagaa attttcacat
      781 aaattcatct gaaattctat aaaaaatgcc ttaattttaa tgaggtaaat cgagtttaca
      841 cagtctaaaa attacttata tttataaatt tatcttatta attaaggaac tttcttaaac
      901 atcttcaaaa ataaagtatt ttagtctata atctttaata ttacacaaac aataaaggag
      961 aagaatagag tatgcttaaa aagtttaata aaatactagt ggctaataga ggcgaaatag
     1021 ctataagaat atttagagcc tgttcagaat tggggataaa aagtgttggt atatatagta
     1081 aggaagataa atatggctta tttagaacaa aagctgatga atcatatttg ataggagaag
     1141 gtaaagggcc aatagatgca tatctagata tggatggaat aatagaccta gcaaagagaa
     1201 aaaaagttga tgctatacat ccaggatatg gttttctagc ggagaatgca gaatttgctc
     1261 gtaaatgtga agagaatgga attactttta taggaccatc atctaaagta atgaatatga
     1321 tgggggataa gattaactct aaaaaaatcg caaaagaagt taatgttcaa actataccag
     1381 gagtagaaaa agcaataaga agtactgaag aagcaaagga agttgcaaac aaaataggct
     1441 atcctgttat gataaaagca tctaatggtg gtggcggtag aggtatgaga attgtacatc
     1501 acgaagaaga cttagagctt gaatatgaaa ctgcttgcag tgaatcaaga aaagcttttg
     1561 gagaagatat tatatttata gaaaaatata tagctgatcc aaaacatata gaagttcaaa
     1621 tattaggaga taactatgga aatatagttc acttatatga aagagattgt tctgttcaaa
     1681 gaagacatca aaaaataata gaatatgcac cagctttttc gctagatgat aaagtaagaa
     1741 aagagatatg tgaagatgca gttaagctat caaaacatgt gggttattct aatgcaggaa
     1801 cattagaatt tttagtagat gcaaatggcg gacattattt tatagagatg aacacaagag
     1861 tacaagttga acatactgta acagagatgg ttacaggaat agatatagtt caaagtcaaa
     1921 tactaatagc ccaaggttac agtttagata gtgaagaaat caatataaaa tcacaagatg
     1981 atgtggaaat aagaggttac tctatccaat gtagaataac tacagaagac cctaagaata
     2041 aattcatgcc tgatacaggt aaaattcaag tttatagaac tggttcaggg tttggtataa
     2101 gactagatgg aggaaatgga tttacagggg caaacataag tcctcactat gatagtttat
     2161 tggttaagac aatatcttgg gatagaacat tccaaggagc aataaataaa actataagat
     2221 ctataaaaga acttagagtt agaggtgtta aaactaatgt aggattccta gttaatgttt
     2281 taaataatcc tatattctca aatggtaaat gtagtactaa atttatagat gaaaacccag
     2341 atttatttga aataacagaa agtaaagaca gaggaacaaa attacttcag tttataggag
     2401 atgtaattgt aaatgataat gcttgcaagg aaaagccatt atttgatgca ttacatgacc
     2461 caagaatgga taaggatggt tcaaaagcag aaggaagtaa aatacttttt gataagttag
     2521 gtaaatctgc atatatagaa aagataaaaa atgataagaa attattatta acagatacaa
     2581 caatgagaga cgcacatcaa tcattattag caacaagaat aagaacatat gacttattaa
     2641 aagctgctaa gccaacagaa aaatatcaaa aagatttatt ctcacttgaa atgtggggag
     2701 gagctactta tgatgttgca tatagatttt taaaagaatc accttggcgt agattacaaa
     2761 aactaagaga agaaatacca agtataatgt tccagatgtt gcttagagca tcaaatggag
     2821 ttggatataa aaactaccca gataatgtaa tagaagaatt tacaaaagaa tctgcgagac
     2881 aaggtataga tgtatttaga atatttgact cattaaactg ggttgaaaat atgaaaccat
     2941 ctataaatac agctttagaa actggaaaaa ttgttgaagc aactatgtgt tatacaggtg
     3001 atatactaga taagactaaa actaagtata atctagaata ttatattaaa atggcacaag
     3061 aattagaaag cttaggagca gatataatag caataaaaga tatgtcagga cttcttaaac
     3121 catattcagc atacactcta gttaaagagt taaagaaaaa tataaaagca ccaatacatt
     3181 tacacactca tgatacaagt ggaaatggtg tagcaacttg tttaatggca tcagaagctg
     3241 gtgtagatat aattgacgct gcattagaat ctatggcagg gcttacaagt caaccatcac
     3301 ttaatgctat agtagaggct cttaaaaata cagaaagaga tacaggtata gatttatttg
     3361 gatatgatga gttaggtaaa tattataagg atttaagaaa agtttatagc aaatttgaaa
     3421 gtgatttaac gaactcttgc gcagaaatat ataattttga aataccagga ggtcaatata
     3481 caaacttaaa acctcaagct gatagtttag gattagttaa tagatttgat gaagttaaag
     3541 aaaaatataa agaagctaat gaagttgtag gagatataat aaaagtaact ccatcttcaa
     3601 aggttgtagg agatttagct atctttatga ctaaaaataa attagataaa gacaatataa
     3661 tagaagaagg taaaaattta tcattcccag attctgttgt ggattattgt aaaggtatga
     3721 ttggtcaacc agaaggtgga ataccaaaag atttacaaga agtagttcta aaaggtgaag
     3781 aagctatcac agtaagacca ggttcattat taccagaaga agattttgat gaaatagcta
     3841 aatatctaaa tgaaaaatat gatataaatg ctaacataag aaatgttata agttacgctt
     3901 tatatccaaa agtatatgaa gattatatca aacatttaca acattataat gatatatcta
     3961 aacttgaaag tgatgtattc ttctatggat taaataaaaa tgaagaatgt gaagtggaaa
     4021 tagaagaagg taaagtatta acaataagat tagttgaaat aggtgaagtt aaggaaaatg
     4081 gatttagaac tataggattt gagctaaatg gaatggttag agaagtagaa ataaaagata
     4141 agaacttctc tggaaaaatt aataatgttg aaaaagctga tatgaatgac cctcttcaaa
     4201 ttggagcaag cataccagga aaagttataa aaataatggt taaagaagaa gatgaagtaa
     4261 aagcaaatca accattaatc gttatagagg ctatgaaaat ggaaactata atagttgcca
     4321 agactgatgg agtgattaag tcaatcaaag taaaagaaga tgatatggtt gaagacaaac
     4381 aattattaat gataatgaag taattaaatt aaaataaact aaaaagcact ctgaaatgtt
     4441 taataaaaaa tgttttagag tgtttttatt tattttaatt aaatatttgt taaaaaaaag
     4501 aggacaatca tgtccatatg tagaaataaa gttagtaagg aaaatgttat ctaaaaattc
     4561 taaatatagt aaatattttc cgtaatattt cttaggaaat aacagcaaag agaaaataca
     4621 taaatttaaa aggtgggtgc ttatatgaag gtttatgata gtaaaatgtt gagaaatgta
     4681 gcggtattag gacatagcgg atgtggaaaa acaaatctaa tcgaaactat agcttacaca
     4741 gcaaacacta ataagatacc taaattaaca gacaaggtaa atatgactta tagcatggga
     4801 ttaataccta tagagtataa tgattataag tttaacttat tggatacccc aggatatttt
     4861 gactttagcg gagacgttgt gtcatctctt agagcaagtg atgcagcaat aatagtaata
     4921 gatgcaacag cgcctattca agttggtaca gagaaatcat tagaactaac tgagagtatt
     4981 cctaagataa tgtttataaa taaaatagat aatgaaaaag caagatataa agacgcaata
     5041 gcaatgttaa gagaaaagta caacaataag atagttccta tgataagtcc tatatacaaa
     5101 gataaaaact ttgtaaagtt acataatgtt tttgaaaaca tagatgattt agaaggtgaa
     5161 tttaaggaac aagctatgag tgttaaagaa gcattaatgg aacttatagc agagacagat
     5221 gaccaaatat tagataaata ttttaatgga gaagaattaa ctacagaaga aatacaaaaa
     5281 ggtattatta tagggataca aagaggagat attatacctg taatatgtgg ttcaactata
     5341 aataatatag gaacaaaaga aatattagat actatatcaa attacctaga acctattttt
     5401 acggaagaaa gtaaaccatt tagaggatta gtatttaaga ctatggttga cccatttgtt
     5461 ggtaaaatgt catatataaa aattactgaa ggtgtacttt ctaaagataa agatgtcttt
     5521 aatataaata aaaatgtaaa ggaaaaaata gctaatatat atacattgaa aaatagtgaa
     5581 ttagtggaag tagaaaaagc taaagctgga gatatagtag tcgtaactaa aattaactct
     5641 ctaaaaacag gagataccat atctatagat aaagatgcag aagctttgga aaatatagat
     5701 ttccctaagc cacagatata ctatgcagta actcctaaga ataaaggtga tgaagaaaaa
     5761 gtagcatctg tattaaatag actagtagaa gaagacccta cattgcattc ttatagaaac
     5821 acagagacaa aacaagcact tttaggaggg caaggagaac ttcatataaa aacaattaaa
     5881 aataaaatga aagataaatt tggtgttgat gttgaattaa atgaccttaa agttccttat
     5941 agagaaacta taaaaggaaa atcagatgta caaggaaaac ataagaaaca atcaggtggt
     6001 catggtcaat atggagatgt aaaaataaga tttgaaagat gtgaatctga ttttgaattt
     6061 gcagaagaga tatttggagg ttctgtacca aaacaatata tacctgcagt tgaaaaaggt
     6121 ataaaagact ctatgcaaaa agggatatta gcaggttatc ctgttacaaa cataaaagcc
     6181 acgctttatg atggttcata ccatgatgta gactcttctg aaatggcctt taaaatggca
     6241 gcaagtgtag cttttaaaaa aggtatggaa gaagcacatc caatactact agagcctata
     6301 atgaaactta agataactgt tccagaagaa tatatgggag atgttatggg tgatataaat
     6361 aagagaagag gaaaaatatt tggaatggaa ccagatgaca aaggtaaaca aataatattt
     6421 gcagaagctc cacaggcaga gacatttaaa tatgcaatag atttaagagc aatgacacaa
     6481 ggcagaggat actttgaaat ggagctagag agatatggag aagttccatc acagtttgcg
     6541 gaaaaaataa ttgggttagc tactgcaaag taatgaacaa aaataaatag aataataagt
     6601 aattaataga tactattaaa aatatgttta ggattttaac taggtataag tttatgttaa
     6661 gtaaacttat acctagtttt tatatgtttt taatagataa aaaaatataa aatactttct
     6721 tgaatatttt aataaaaaga aagtatttta ttattattat ttttacaaat gtttagcaat
     6781 aaagatgaga aggtatattt aaaatataga gtatttaaaa tgttgaaatc taatcatgaa
     6841 gcaaaaaaat agagtttggt tatcataaat atagtaaata aaaactcgta attaatgaaa
     6901 gaataaattc atatctaaat ttctataaaa aatttaaatt tatacaattt agggctttaa
     6961 aaatttaaat aataggtgta aaataatagt caaagaaagt caaaaataaa aaggtgtgtg
     7021 gtgatattaa tggcaactat gacagatata attgaaaaat ttattaaaga tttgatggaa
     7081 gaagataata gcatacaaat tcaaagaaac gaactagcaa acttgtttag ttgtgcacca
     7141 tcacaaataa attatgtctt aacaacaaga tttacaatag atagaggata ttacattgaa
     7201 agtaaaaaag gtggcggagg atatgttcaa atagaaaaaa tacgaaaaag taaagatgga
     7261 catattagag aactgttaaa tgaaaaaata ggaaatcaaa tatcctataa aaaagcaaaa
     7321 gaattattgg aaggtttaaa agagtcagac ttgattaacg aaagagaatt aaaattaata
     7381 ttatatgcaa tagatgataa atcattatgt atgccaatat atgaattaaa agagaaggta
     7441 aggtctaata ttttaaagaa tataataata ggattattta gtatagagga gtgataattt
     7501 atgctatgtc aaaaatgcaa taagaataaa gcgtctgtat attataataa gatagtcaat
     7561 ggagaaaaaa cagagatgta tctatgtagt gagtgtgcaa aagagaatac agaaatgaat
     7621 tttaatttag atatgccttt ttcaatgatg gatatatttt ctaatttagg ttttcaacct
     7681 aaaaaagaac ttgaggaaaa gttagtttgc cctaaatgta atacaacata tagtgaattt
     7741 aagaataatg gtagatttgg atgtagtgaa tgttacaatg catttagtag tcaagtaaat
     7801 ccaatgcttc aaaatataca tgggcatata gaacatacag gaaaagcacc taaaaaatct
     7861 ttttataaaa taagtgtaga aaatgagatt aaagaattaa aagaagattt agatagagct
     7921 ataaaaaatg aagaatatga attagcggct caatttagag ataaaattaa atacttaaaa
     7981 ggttctattg actaagattg gagtgatatt atgaaagaga acatagtcat gaaatcaaga
     8041 gttaggctag ctagaaattt aaataactat tcctttccaa ataaattaga taaagaatgt
     8101 gccatggaaa taatagaaaa agtaaaaaat gcatttataa attctaactt agaacaaaaa
     8161 gaagaatttg atttctataa aattgaagat ttagaccaat ctaaaaaaat gcttatggtt
     8221 gaagaacata taataagccc ggatttagca gaaagtgata aaagtgcagt tatagtaaaa
     8281 aaagataaaa cggtaagcat aatgataaat gaagaagacc atataaggat acaaactatg
     8341 tgtgatgatt taaacttaga atatgcatat agtgtagcaa atgaaataga tgatttgtta
     8401 gaatcaagtt tagaatatgc atttagcaca aagctaggtt atctaacatc ttgtccaact
     8461 aatacaggaa caggaatgag agcctctgta atgatgcatt taccagcact aagtcagcta
     8521 ggttatatgg atgaacttta taaaatttca tcacaaatag gtatagcaat tagaggtata
     8581 tatggagaac gaacagaagc attaggaaat atatatcaaa tatctaatca acttacactt
     8641 ggaagaacag agtctaatat tatagaaaat gtaagtggac ttacaaaaga tgcaatttca
     8701 aaagaaataa aagcaagaga aatattgcaa aaaaaattag gtataaaatt agaggataaa
     8761 atatttagat cgatagggac acttgaaaat tcaagaataa tgagtagtgc agaggcaatg
     8821 agccacctat caaatataaa aatgggaata gaaatgaact atatagataa actagattta
     8881 agagctatag aacaacttat gataggtata caaccagcac atcagtcaat aatgtataag
     8941 agtgatgatg ttgagaatag agatataaat agagcaacat atattagaga aactttggaa
     9001 aaattaagag gagggatgaa ttgtgaactt caatagattt acacaaagag ctaaaaaagc
     9061 tattgattta gcgtttgaat ctgctaaagg tctagggcat aatattgtcg gaagtgaaca
     9121 tatactttta ggtcttttaa gagaagaaga aggtatagct gctaaagttc taagtaaagt
     9181 tggatttaca gaagcttatt tagaaggcaa aatagttgat atggaaggta agggagaaga
     9241 aatctcagaa gatatagtat taagtccaag aagtaaacaa atacttgagt tatcaggcat
     9301 gtttgctaat aaattaaaaa caaattatat tgggactgag catatattat tagcaattat
     9361 tcaagaaggt gaaggaatag ctaataagat tttaaattat gcaggagtaa atgataggac
     9421 tttagcccaa ttaacaattg atatgatggg tattagtgat aaaaatcaat acaaagcaga
     9481 aaatagttac acgggcaacc agaatcaagc agaatctaaa gtacttgata agtatggaag
     9541 aaatcttaca ctatatgcaa agcagaataa aatagaccct gtaataggta gagaaaaaga
     9601 aatccaaaga gtaatacaaa tattaagcag aagaactaaa aataatccag tgttaatagg
     9661 agacccagga gtaggtaaaa cagctatagc tgaaggtctt gctacaaata tagccttagg
     9721 aaatgttcca gaaacactaa aaaacaaaac tttatactct ttagagatgg ggtcattatt
     9781 ggctggggct aagtatagag gagaatttga agaaagaatt aaagaagttg tagatgaagt
     9841 tgttaaaaat ggaaacataa ttttatttat agatgagatg catacaataa taggagctgg
     9901 gtctactgga gaaggctcta tagatgcttc aaatatatta aaaccagctt tagcaagagg
     9961 agaaatacaa gttataggtg caacaacaat tgatgaatat agaaagcatg ttgaaaaaga
    10021 ttctgctctg gaaagaagat tccaaccagt tatggtagat gagccaacta aagaggactc
    10081 aataaaaata ttagaaggat taagagataa atatgaagct catcataaag ttaaaataac
    10141 tgatgatgct ataaaggcgg ctgtagaatt atcaactaga tatatatcag acagatatct
    10201 gccagataaa gctatagact tgattgatga agctgcatca aaagtaagat taaaagaaaa
    10261 tacacctcca tcagagataa aaaaattaga actagaaata gaaaatatag ataaagaaaa
    10321 agaagaagca gtaagatgtc aagactttga gaaagcagcc aaaataagag atgaacaagg
    10381 tctacttaaa aaacaattag aagatgttag agagagatgg agcaagtcat ctaagcattc
    10441 agatttagtt gatggagaag ttattgctga agtagtaggc ttatggacag gaatacctgt
    10501 taataaaatt cttgaagaag aggctgatag acttttaaga ctcgaggaaa tactacacaa
    10561 tagagttata ggccaagaac aagcagtaaa atctatctca aaggcaatca gaaggtcaag
    10621 agcaggtctt aaagacccta atagaccaat tggttcattt ttatttttag gtccaacagg
    10681 agtaggtaaa acagaattat ctaaggcatt ggcagaagta caatttggag atgaaaatca
    10741 aataattaga atagatatgt ctgaatatat ggaaaagcat gctgtttcaa gaatgatagg
    10801 ttcacctcca ggctatgtag gtcatgatga aggtggtcaa ttaactgaaa aagtaagaag
    10861 aaatccatat tcagttatct tatttgacga gatagaaaaa gctcatccag atgtatttaa
    10921 tattctatta caaatcctag atgatggtag actaactgat tcaaaaggaa gaactgtaga
    10981 ctttaagaat accattgtaa taatgacatc aaatgttggt gcatctacaa ttggtagaca
    11041 aaaaacttta gggtttagta tagctaaagg agacgaagaa gaaaaatctc agtatgaaaa
    11101 aatgaaagaa aatataatgg gtgaattaaa acaaagattc agaccagagt ttttaaacag
    11161 aatagatgat ataattgttt tccattcatt aaatgaaaat catatatcaa aaatagtact
    11221 tttaatggca gcaaaactac aagagagatt aaaagaaatg gatataaaat tagaaatgag
    11281 tgatgaggct gttaaattaa tttctaaatc tggatttgac ttagaatatg gagcaagacc
    11341 tcttaaaaga gctttacaaa aagagttaga agatgagttg tctgaagcaa tcttaaaagg
    11401 caatgtgaaa aagggaagta atgtagtagc aaaagtcaaa gatgaaaaaa tagtttttga
    11461 aactaaataa tttaatagag ttgcatttta aaatattgca aatataagct ttataatcta
    11521 aacaaaattt taagggcaat gaatctataa taattcattg tccttaatta ttttatttaa
    11581 tctttacata ttgtctttca gatatgctaa aattatagta tagaataata gattaaggat
    11641 gataaaagta tggcaaaaat aaaaacaaaa tatgtatgtc aatcttgtgg atatgaaaca
    11701 gctaaatggc ttggtaaatg tccagaatgt acaaaatgga atacattcgt agaagaaata
    11761 gaccaaaaaa gtaccaaaaa agaggtcttt ataatagata aatcttcgtc taaaccagta
    11821 actataaact caatagaaag taaagaagaa gagaggttta caacagatat aaatgaatta
    11881 gatagagtgc ttgggggagg tatagttaaa ggttctttag tacttgttgg tggagaccct
    11941 ggtataggta aatcaacact tttaatccaa gtatctagca atgttgctaa tttaggaaaa
    12001 actgttttat atataactgg agaagaatca gaatctcaaa taaaaatgag agctaaaaga
    12061 ttaggtatta actcagaaaa tttatatata tttgctgaaa ataatttaag tataattgaa
    12121 tcttatttag aagttgtaaa tccagaatta ataattctag actcaattca aactgtcttc
    12181 agtccagaaa tatcctcggc accaggaact gtcagccaaa ttaaagaagg tacttcaaaa
    12241 ttcatgaaaa tttctaaaaa aatgggaatt tcaacattca tagttgggca tgttacaaaa
    12301 gaaggttcac tagcaggacc aaaattatta gagcatatgg ttgatactgt tctatatttt
    12361 gaaggagaaa gatataatac ttatagatta gtaagagctg ttaaaaatcg atttggctca
    12421 actaatgaac ttggagtatt tgaaatgagg gatttaggat tagtggaact tgataatccg
    12481 tctaaaattt taatctctga aaaaccaaaa gatgtagcag gttcagtaat aatatctact
    12541 gtagaaggta cgagaccaat gttacttgaa ttacaagcat tagtttctcc tacaagtttt
    12601 ggtataccaa aaagaacatc tacaggagtc gattacaata gagtaggtat gttattagca
    12661 gtattagaaa aacgtgttgg tttgcaaata caaaatcaag atgtctatat aaacatagtt
    12721 ggaggaataa agataaatga accttcaatt gacttaggga tagctatttc tgtagcatct
    12781 agttttagga atataccaat agatgaagat atagctgtca caggagaagt gggtcttaca
    12841 ggagaagtta gagcagtaag ctttatagaa aagagaatag ctgagtgtaa aaaacttgga
    12901 tttaaaaaga tagtagttcc tagaagtaat tatgatgtta taaaagatac caaaggcata
    12961 gaaatatggc cagtagataa cttaagacaa gctataaata tagtgcttgg gaggaatcaa
    13021 taaatatgga gaattttcta gataataaaa atatgctata tgcattaaaa atgatatctc
    13081 ctggaactcc acttagatta ggtctaaaca atgtactaag agctaagact ggtggattga
    13141 ttgtaattgc aacgaacgaa gatgtaatga aaatagtaga tggaggattt gctataaatg
    13201 cagaatattc accatcatat ctatatgaat tagctaaaat ggatggagct atagttttaa
    13261 gtggtgatgt aaagaaaata ttatttgcta atgcacaact tatacctgat tattttatag
    13321 aaacatcaga gacaggaaca aggcatagaa cagcagaaag agtagcaaag caaactggtg
    13381 ctatagtcat agggatttca caaagaagaa atgttataac agtttataga ggaaatgaga
    13441 agtatgtggt cgaagatata tctaagatat ttactaaggc aaatcaggct atacaaactt
    13501 tagaaaaata taagacagta ttagaccaag ctataacaaa tttaaatgcc ttagagttta
    13561 atgatttggt aactatttat gatgtcgcat tagtcatgca aaagatggaa atggtaatga
    13621 gagttacaag tataattgaa aaatatgtga tagaattggg tgatgaagga actttagtaa
    13681 gtatgcaatt agaagaatta atgggtacga ccagaataga ccagaaacta atattcaaag
    13741 attataataa agaaaataca gaaataaaag aacttatgaa aaaggtcaaa aatttaaatt
    13801 cagaagaact aatagaattg gtcaatatgg caaaactatt agggtatagt ggtttttcag
    13861 aaagtatgga tatgcctata aaaacaagag gctataggat tcttagtaaa atacatagac
    13921 taccaacagc aataatagaa aacttagtaa attattttga aaactttcaa caaattttag
    13981 atgcatctat tgaagaatta gatgaagttg aaggaatagg tgaaataaga gcaacatata
    14041 taaaaaatgg acttataaaa atgaaacaat tagtattatt agatagacac atatgatgaa
    14101 ttttaaatga attttactac ttttttcttg aatataatat acactttggt ctataataaa
    14161 taagtataac tagggggtga agcattgata aggaaagtaa caagaatatt gtttatttta
    14221 cttggattta caataggtat aacaacctat ttaacattaa tgaaagattt tgaaatatta
    14281 acatttggaa aagaaacata tggttatatc gcagctgtag tagcaggtat aataatagcc
    14341 attttaggat attttataga accatgggtt gtaaataaag taaaagaaat tgctaaaata
    14401 gtagataaag aattgtcaaa atatcctcag actgatatat tgttagggtc tatgggtctt
    14461 atagtaggat ttgttattgc atatttatta agtggattag taaatagaat accaatagta
    14521 ggtggaatac tatccttact attatattta ttcctaggat atcttggaat gaaagttgcg
    14581 cttaaaagta aaaatgattt atttaatatt ggaaaactag gaagattagc aaatcctatt
    14641 aaggataaag ataaggataa agaaaataaa aaagatataa aagccattcc tccaaaagta
    14701 ttagatacaa gtgtaataat tgatggaaga atagctgata tttgtaagac tgggtttata
    14761 gaaggaaaac ttattattcc tgcttttgtt ttggaggaac taagacacat tgcagattct
    14821 tcagatgatt taaaaagagt aagaggtaga agaggtctgg atatattaaa tataattcag
    14881 aaagaattaa atatagaagt tgaaataagt gagagagact ttgatgatat agcggaagtt
    14941 gatagtaaat tattaaagct tgctcaggtt ttaaatggaa aagttgttac aaatgattat
    15001 aacttaaata aagttgctca attccaaggt gttgaagtat taaatataaa tgagttagct
    15061 aatgctataa aaccagttgc tataccaggt gaggatatgg ttgttcaagt ggttaaagaa
    15121 ggaaaagagg ctcaacaagg tgttgcatat ctagatgatg gaactatgat agttgttgat
    15181 ggaggaagaa aatatatgaa tgagactata aaagtacttg ttacttctgt tcttcaaact
    15241 cctgcaggta gaatgatatt tgcaaaacct aaaaattaat atgttcaaag aaacctataa
    15301 tttgggctgt ttattttaaa tagatggtcc aaatttattt atttataacg ataatgttta
    15361 atgtattctt aaagatgggg atttaaatca gaaagcaggg agaatttatg agaaaaaaga
    15421 ttatatcttt cttagcaaca tttatcataa tattaacatc agcaagtcag tatatttttg
    15481 cagatgatat atcaacacgt ggaaaagtaa tatttataga tatgaataga actagtatga
    15541 gtaatatgtt aagaataaaa tcattaagag aagaactaga caatagaggg tatatcggtt
    15601 tgatgaatat aagaggcgat aaaggaagtg atgatagacg gtcatatgct agtatgggtg
    15661 ctggtggtag agctaatgtg gctaatgaag aagatattaa ttttgaaagc tcgagtaagg
    15721 atagaaatat agtttttgaa tcagctacag gaaagagcgc aaaaggtata aataatttaa
    15781 ctataaataa atctgtaaat gaaaatttaa actttggaga gtatggttct gtattagggt
    15841 cattaggaca aagtttatct gataatggac taaaagcatc tgtattggga aattcagata
    15901 taattgaaaa tggccaactt ataaaaaata gaaacttatg tctaactgca atggatgaat
    15961 atggaagaat acctaatgga aatgtagata ctataaataa aaaagactta tctatgcctt
    16021 atggaatatc aactgattat gataaattga ttgtagagac caaagaagct tacaaaaata
    16081 atgatgtggt ttttgtggag cttggagata catatagatt agatttatat aaaccaaatt
    16141 taaatgaaaa aacatatgaa aatatgaaaa ataaaataga aaaaaatata gatgtatatt
    16201 taaaaaatat attttctatg gttggagaaa atgacactat ctacatagca agtgcatttc
    16261 caagtgattt agattataaa aataaaagaa gactttctcc aatagtaaaa ttaaatggag
    16321 tagggaaagg tctattatca tcttcaacta ctagaagaga aggtattgta acaaatttag
    16381 atgttggggt tgaaatatta gacaacttta atataaaaaa tcaaaatatg gttggaagaa
    16441 aatatgaact tattaacaga gatgataata aagaattttt gatggatgaa tatcaaaaga
    16501 tagtatctat ctcaagcata aggtctacaa ttctgaatgg ttttgtaagc atagttttct
    16561 tgtcatggat agttgctatg attgcaattt tatttagaaa gcatatatca aaaaaatata
    16621 aagaaacaac attttttata ttaaaggaac ttttaaaaat aggaatagta atgccactgt
    16681 catttatgat aacaccaata atgaatttca aaacaccatt agcaatatct ttaggaataa
    16741 ttataataac acttactata tatcttataa gtaaagtttt aataaagaat gatttaaaaa
    16801 acatgctgtt ttttactgga ttaactatag taattatggt tattgatgct ggatttggct
    16861 catatttaat gaaaagcaat gttatgagtt atgactgtat aattggagct agatattatg
    16921 gagtaggaaa tgagtatcaa ggtgtggcaa ttggtagtgc aatatttaca tttgcaattt
    16981 tacttacata taaaaacata ccaaagtggt ctgtaatagt tttttctttg cttatcttaa
    17041 ttacaagtgc atctccaatt atgggagcaa atgttggaag tgctatttct gaatgtgttg
    17101 catttttact atttatacta ttaatatata atgtgaagat agacttcaaa aaaattgtat
    17161 tattaggtat agcagtatta tttgtattag gagcttttgt agcaatagat atgattttgg
    17221 gaagtaattc acatttaggt atgtttgtaa aagaaattta ctttaatgga ccaggagaga
    17281 taatacaaac atttagtaga aagatagaga tgaatttaaa gctagctcaa accagtgcat
    17341 gggtaaatat actgttaact ggaatagggg taattttagc tcttatgatt aatcagatta
    17401 gatactttaa gcaacttatg gatgagtatc caattgtatt taaagggttt atagcatcaa
    17461 tagcgggatg tttagtaact cttttagtaa atgattctgg gattgtgtca tcagctacag
    17521 cctttattta tataattgta cctatgataa cattaagtgt aaatctcaca gcattaaaag
    17581 aataagcact atatgagcac tatgtaaata tgaacacatt aatttaactt ccttctttat
    17641 tttataaaga aggaagttaa taaaaaataa attataataa aaaagagttt atctcaaaat
    17701 tgatttttct aatcaaattt tggggtaaac tttttttatt ataaaggcaa ataacatata
    17761 atcttatatt ttaggtagat tttttcttat atgtttaggg gtacatttaa atttttcttt
    17821 aaaaatccta taaaataatt ttgtattagt aaatccatga gtttctaata tactgcataa
    17881 ggtatcattt gtattaatta agtctttata aatatgagaa atacgtacat catttaaata
    17941 ctctaagtaa gtttgcccca tatattgttt aaattttcta caaaaatatt ctggttgtaa
    18001 gtgtgccacc ttagcaattt tgttaataga aataggagat ttgtaattaa tatttgtata
    18061 ttctaaaact ggttcaaatt ttgacaaatc aagtactgtt tttttcatat ttttattacc
    18121 tacagatatt ttaaagtttg tatataattc aaataaaagt tcaaataata agcttgtaaa
    18181 ttgcagatta gctgctggag gagaactaag tttaatttct ctcatgtttt ctaatataag
    18241 tttaacttta cttgaatttc ttttatagtt attgtcatta gcattaacct taaaatcaaa
    18301 gtagcaattt ttaaaatcag gaatgtattt gtttagaaaa gacattggta cttgcaataa
    18361 tatagaggta ttatgataag tacatctagt tgagtgaata attccagaat taatgaacat
    18421 acactcgtta gcttttaagt tataagtgta accttccatg ttaatttgta aatcaccaga
    18481 ggttatataa attatttcta atgagttatg ccaatgattt gctacataac taccactatc
    18541 atcaaatagt ttaaaattaa catctaaatt atcaataggt tcaattattt catgtgaaaa
    18601 actatttctg ttcataataa tctcctcctt ataactttaa attataacta gtaaatttat
    18661 aatattcaat attaaaaaga taatatagac taataaatta gacaataaac atacttaaga
    18721 aaacagataa aatcaaatat aataactgta tatttaaata atttaaaaaa attattttag
    18781 tataagtgta aaatatacct atttaaataa gataagaggg gaggacttta tgtgtatata
    18841 aaaggtgtaa acttaggagg ttggttagtt ttagaaaaat ggatgacccc aagtttattt
    18901 gaaggtacag aagccgaaga tgaatactac ttaccaagac aactttcaag agatgtatat
    18961 gaatctaaaa ttaagactca tcgtagtgaa tacatcacag agagagattt tgcaataatt
    19021 aaatctatgg gatttaattc agttcgtata ccagtaccat attttatttt tggagattgt
    19081 gaacccttta taggttgtgt taaagaactt gataaagcat tttattgggc agataagtat
    19141 ggacttagta tattgataga tttacataca gttccaggaa gccaaaatgg atttgataat
    19201 ggaggaatat caggggtatg tagctggtca caaaacccag aatatgtaga gtttacatta
    19261 aatgtactag aaagacttgc aaaacgttat gggatgagac gtgaattgta tggaatccaa
    19321 attttaaatg aacccataac agaaagaatg tggaatataa tgaatgttcc aaatcgattt
    19381 aaggctgtag ataaggatat ggcaagaaga agtaaaccta attcattaga atttttacgt
    19441 gatttttata taaaagcata taaagtaatg cgtccttata tgagtgagga aaacgttata
    19501 gtatttcatg atgcatttga attaaaatca tggaaagact ttatgaaaga ggaagaattt
    19561 aaaaatgttg ttctagatac acaccaatat ttgatgttag ctgaagcaga tggatgtgaa
    19621 caaagtattg atagttatct caaatatatt agagataatt atgctaaaga tattttacaa
    19681 atgcaaaagt attttcctgt tatatgtgga gaatggagct tatttaattc atatgcttgt
    19741 ggtatagata cagctggagg tcaatcgcct ctaaatggga ttgaatcaaa tatagataaa
    19801 ttatcaaaag atgaaagaag agaattatat aggaagatag caaaagcaca gattgatgca
    19861 tggagaaatg gaagtggtca ctattattgg aactataagt tattactaga tacagttaat
    19921 gaagaaggct ggataggttg ggatagttgg gacctaggca aatgtgtagc tcaagaatgg
    19981 tatcctatag aatattaaac taaaattgga ggaaaagata tgattaaaaa ccctatactt
    20041 ccaggcttta atccagatcc ttgtatttgt agaaagggag atgattatta tcttgtagtt
    20101 tcatcttttg aatggtttcc aggtatacct gtttatcact ctaaagattt aaaaaattgg
    20161 gagctataca ctcatatttt aacagatgaa acaaaaatag atttaaagaa gcttccttca
    20221 tctaaaggaa tttgggcacc ttgtttgact tattgtgaag aagaagattt attttacata
    20281 gtttttggaa ttatgaactc tatgaatgca agatattttg atgttgataa ctatttaatt
    20341 acatcaaaag atataaaggg agagtggagt gaaccagtat atctacattc atcaggtttt
    20401 gatgcgtcta tttttcatga tgataatggt aaaaagtgga tttcgtcatt agactgggag
    20461 actagagaag gatatgaaaa acctggtgtt atatgtttag tagaatactg tatagaaaaa
    20521 aaagaaatag taggatatcc aaagagaata tggtcaggag gaactgatag aggttgtatt
    20581 gaggcaccac atataaccaa gcgtggagat tattattata taatgtgtgc agaaggtgga
    20641 actggatatg gtcatggtgt aactatggga agatctaaaa atatatgggg accttatgaa
    20701 aaagatttaa tgaatccaat tgtaacttca attccaggag atttttatga aaggcatgac
    20761 cctgaccatc taaagcctaa atactataat ccagaatcaa agcttcagaa atctggtcat
    20821 ggaagctata tagaaacacc atcaggtgaa gtgtatttag ttcatctcac atctagacca
    20881 tttgtaccag agttaaggtg tactttaggt agagaaacag ctattcaaaa aatgaaatgg
    20941 acagaggata attggcttcg aatggaagat ggaagtaatt tggcaaaaga atatgtatca
    21001 gaaagtaagc tagaagagca tttagtttca tctataccaa gttttgatga ctttgattca
    21061 aataaattag gtttacaata ctatgcccct aggatttcgc cattatcatt tgcagatgtt
    21121 aattctagaa caggatatgt aaggattaga gggcaagaat caagaacatc attaaataga
    21181 gtgagcatat tagcaagaaa gttgacttca gtatatgcaa gaataactac taaaatggag
    21241 ttttatccag aggtacatca gcatagcgca ggtttgataa tgtattatga taatatgaat
    21301 tatataaatt taagaaagta ctatagtgaa acattgggtc aaagtgcact atcaataata
    21361 catcttgaaa atggagagaa aacagagttt ttaaatacta ggataccaat aaaagatata
    21421 ccaatatatc ttcgtctgta tataaaaggt cgtaagtcgt atttcgaatg gagttatgat
    21481 ggagaaaagt accaaagaat aggagagaca tttgatacaa ctaagttttc agatgagtac
    21541 tgtaaatatg gagaatttac aggtacattt ataggattaa cttgtgcaga tcgtgtaaag
    21601 cataaacact atgctgattt tgatttcttt gaatatatag cagacgaatc aaaagatgta
    21661 gattaataaa attattacta aaatttttag agcataaggt aaaacttatg ctctgaaata
    21721 agaaacatta gaaggtaaat cttacaagaa ggagtatggt attatgaata aagaggttaa
    21781 tgttaaagca actactatta aatatgaaaa gatttcattt tttgaaagag tttcttatgg
    21841 gtgtggggat ttaggatgca atatcatata ttcagcaatg tcagcatttt tattgtttta
    21901 ttacactaat tatgcagatg ttagtgcagc tgctgtaggt agtattatgc ttatatctag
    21961 gatattggat gggtttagtg acttgacaat gggaattata gtggatagaa ctaaatctaa
    22021 atatggtaag gctaggcctt ggattttaag aatggcgatt ccttttgcca tatcagcaat
    22081 attattattt tctgttccat caagtcttgg tgtcacttct aaattaattt atatttttat
    22141 tacatataac ttagtttcta ctgttattta tacagcaatc aatgtgccct atgcaactct
    22201 aaattcactt ataactcaag accagtatga aagaggagta ttaagtatat ttagaatgat
    22261 attagccact tgtggtactt taataataaa tggacttaca ttacctctag ttgagcattt
    22321 tggaaataat ttatcagcat ggactaaaac gttttttata tttggaatag catctataat
    22381 agtatttttt ataacattta ctggaactaa ggaaagagta aaagcagtta agcaaaataa
    22441 gaatgaagta ataccattta aaatcggtat taaatcttta tttagaaata agtactggat
    22501 acaaataaca ttatgtttag tttgtatatt tattgtattt gcaataaatg gtggttcatc
    22561 tgtatattat gcaaagttta ttttaggaga tgaaaaatta tttgctccta taaacatggt
    22621 ttcaaacgta tctcaaatta tagctatgtt tatggttgct ccttttataa aaaaatttgg
    22681 gaaaagaaat gtattaatag taggttcaat aatattaata tcatcaaatg ttatgtttat
    22741 aatagctgga caaaactata ttggagtaat atctgcaagt gctataaagg gaatagggag
    22801 tgctggaata gcagcaacga tgtttgctat tgtttcagat actattgaat atggagaatg
    22861 gaagactgga tatagaactg aaggtcttat aaatagtgca tctagttttg ggtttaaagt
    22921 cggaaatgga ctaggttcag caatattagg tggagtatta tcaattggtg gttatgtagg
    22981 gacatcagct actcaaagta attcagctat agtatcaata aaagcatgtt ttatatacct
    23041 acctatattt ataaccatat tacaaattat aataatgttt ttttataagc ttgacaaaga
    23101 atatagtact atacttaatg aattaaatac taaataataa aatattttct attcagtctt
    23161 agatttaatt aaggattatt atagtttatt atttatgaaa aactattata tattcatgat
    23221 atattaaagc gtttttagaa aaataaaaaa gaaaaactaa tactaaaaat ataaatatat
    23281 ccagtaaaaa acatcttaaa atctatttat ttggattcta aaagaataaa ggagatacct
    23341 taaaataaaa atttgagtta cctccttttt taggcaataa tttaaggttt atgttgtttc
    23401 aacatgattt gaaatgatgt accctttccc ataatgcttg aaactgaaat atttccttca
    23461 tgtgcatcaa taatttgctt aacaataaat aatcctaacc ccttattttg agtattttgg
    23521 tttttgctac tataatactt agcaaaaata tcatttaatg tttctggatt tatcccaata
    23581 ccattatcat taatattaat gtaaatttca tcagattttt gtgttaagac aacatctatt
    23641 tctgttccag aaggattgtg ttcaacacaa ttaattaaaa ggttataaat agctctatac
    23701 attagtgtct catcaataac caaatttaaa tgttgtaaat ccgtatgaaa ttcaaatata
    23761 tgtttttctg atttcgggtt attggttata tcagcaataa ggtttcgaag aaaagaaact
    23821 atttcaactt gtgtgtgttt aagatgtatt ttgttatgtg aatctataaa aaacgtcaga
    23881 cttaactcat ctattaattt ttcaatatat attccctttg tatatatatt atttaaaaag
    23941 gttaacatct cttcttcact aaaactgtgg tttttgttaa gcattagaga tgaatatcca
    24001 gtgatatatg acaagggggt ttttaaatca tgcgacacac tagcaagcca atcagttttt
    24061 gcaacttcaa gtttttctcg ttcaatctca gtttcttgaa gcttgttgcc taagtctttt
    24121 atatttataa taacttctct gtacataaag ttataccatt ttagctttcc atttttagta
    24181 aaagcttttt cagatattgt atagtttcct ctagataagt tgcttattga actaattatc
    24241 aggtagagtg gtcgaacaag aaataaacaa aatagtattc ctcctgtaat aaagcaaaaa
    24301 gtaaatataa gtgtgcttgg gaaatagata atagacgaat caattacata taaaaattta
    24361 tgtgaatctt cagggtatat taattgatta agcaatatga ataatgttat gaagtatact
    24421 aggaatataa agagacctgt tgtaaagtaa gcaatgaaac gaaaatataa ttttttcaat
    24481 atatcagttc ccctttggcg gtatgaattt atacccaatt ccacgaaggt ttgtaataac
    24541 cttgtatggt ttattgtcat ccagtatctt ttttctaagt ttggatatat gtattgtaac
    24601 tgtcttttca tcgccatatc cttgtaatcc ccaaatttta tcatacaggt gtgatgtagt
    24661 aaagacccta tttggatttt gacaaaaaaa ttctagtagt tctaattctt tggctgtaca
    24721 ttctacattt ttaccactta cagtaagtag tgcatgagcc atatcaagaa caatatatcc
    24781 ataattataa actaaaatat tttctgagtg attacataat acagaacgtt cgagttgttg
    24841 acgtctaaaa atagctttga ttctagcaac aagttccatt gtattaaatg gttttgtaat
    24901 ataatcgtca ccaccaatag caagtcctga taatttatca taatcactag ctcttgcagt
    24961 tagaaaaatg ataggtgcta tagttttttt gcgaaaattt gtacataagt caaatccact
    25021 ataatctggt agcattacat caagaatgat aaggtcatat tgattatttt ctatacattc
    25081 aagggctttg gcccctgttg atacacaggt aatattattg tattgctctt tatttaaggt
    25141 tattttcaat agtgttaaaa gtccttcttc atcgtcaaca ataagtattc tttcagagtt
    25201 tttaaatttg tcattcataa taattcatct cccagtatac aaaaatagta tcattataat
    25261 accatacatg caaagtgtaa gcaataaatt attcttttga ttttacttta actttacttt
    25321 tattttactt ttgtactgta tcttatagat aacaagaaag aaaaaaataa aaataggagg
    25381 taaaaatgat aaaatcactg aaatcaagag gaataagtat tctattatgt ggagctttga
    25441 ttgttggtgt aatgacagga tgtacatcta aagctacatc cccaaaaagc tcaacaccta
    25501 aatcaactcc taaaacagta gaaaaagtta cacgtgcaaa ttgtgaaaaa caactaaagg
    25561 aagtaaaagc aaagtataag aaaaatccaa atgatgaagc tatcagatta gaatatgctc
    25621 aaatactctt taagttggga aatattacag aagcacaaga aattctgaat ccgttactaa
    25681 attcaaagaa gccttcacca gatgctattt ttctttctgc acgaatagaa tatctgaatg
    25741 gtaattatac tcaggctgaa aaactatatg ataatcttat agagcaatat cctgataaat
    25801 tcaaatcgaa ggctgaagac gagttggcac ttacgtatta tcaaacaaat caatatcaaa
    25861 aagctgacaa gttatcctcc gtgaaaacaa ataatgctct attggaaatg atgaaagctt
    25921 ttaaggataa acatccaaat aagattgact ggaatggaaa aaaagaaact gtaattccat
    25981 ttgtaacagg tgacccacta cctattatac ctatggaaat aaatggtaaa agaataaata
    26041 caattattga taatggtgct ccaatgtttg ttgttgatga gaaaattgca gaagaaattg
    26101 gtattaatct agcttcaaaa gaggaaaata ctaaatttgc tggtggtaat agtattggaa
    26161 ttaattttgg tcaagccaat tcacttacca taggagatgt aaacatggag aatattcctg
    26221 taatgttagg accatttgat ggatttgaaa agctgtttaa agactatgga aatgttcatg
    26281 gaataatggg aacaactgta ttaaaggagt ttgtacctac aatggactat cctagtgggc
    26341 agctaatatt aagacctaga aatgaagtag gcagagataa tcttaataag atgcttacaa
    26401 atgataaaat attagaagaa gttccattta cacttgcttc tactcatttt atgtttggaa
    26461 aaggttcaat caatcaaaag gttgggttaa atttctttgt tgattcaggt cttggtgacg
    26521 aaaaaggagc aggaattgta ttaactgagg aaataatgaa ttccttgaaa attcctatgc
    26581 ctgagctaaa gaaagcagag gaaggaaaag gtggaatggc tggaagtgat tataaagaag
    26641 gatatttcaa tctttcatct tatggtctag gagatttgca acttaaaaac gtccttggat
    26701 attattttac aggtgatacg ttggattatg atgaggggat tggatttgtt agagatggat
    26761 tgataagtca tcactattta aagaactata aatggtctat tgactttgat tctatgaaga
    26821 tgacatttag taagtaaaga tagtatataa tatgaattaa gtgggaaagt ttcaactgtt
    26881 caaggaaacg gtgatacaag aaaagtatca ccgttttttt tattatacaa aaaacagttg
    26941 actttttata tgaacccctc ctataatgtt actgaaatta agtaaaatga ttgaacaaat
    27001 gtaaagataa ttgaaaaagc ttgattttgg agggaagcaa atgaaaaaaa ttggagactt
    27061 aagattaatg atgaagtgtt gttcacttta ttatgaagat aatttgaatc agcaggaaat
    27121 agcaaatcaa ttgggaatat caagaccaac aatatcaaga atattaaaag aagcttttga
    27181 acaagggata gtaaaaatac agatagttga tgttttgaaa aatgattacc aaaaaataga
    27241 gagaagtttg gagagaaaat ataaattaaa agaagtaata gtagtagatg ataaacagga
    27301 ttcactaaca caaaaacaag aattagcaag agctgtatct gaatatttaa ccagggtagt
    27361 aaaggaaaat gatataatag gtgtttcaat aggaacaaca ctaaaagaaa taccaagata
    27421 tgtggaaaaa agtaactgta aaaatgttac atttatacct ctattaggag ggattggaga
    27481 caatgaaata gatattcatg caaatcaaat agcagtaagt ttagcaaggg cctttggagg
    27541 tgattttaaa ttattacatg ctccagctgt tatgtctgac ttatctacaa aagagaaact
    27601 ttgcaaagac gaaaaaatta aagaagtact agatttaata gataaaacaa ctatagcaat
    27661 tgtgggtata ggaaatccta tgagtttaaa ctcaaccata atggcttctg gatatatgaa
    27721 tgaagcagat atagaagatt taaaaaaata caactcaata ggtgcaattt gtttgcaagc
    27781 ttttgataaa gaagggaaaa cttctatact tgaatttaat caaagagtat taggagtaaa
    27841 actggaagat ttaaaaaaga taaaaagaac aataggtgtc gcttcaggag atgaaaaaat
    27901 agaggcaata aaggcatcat taaaagcgaa atttataaat agtttagcaa ttaatcacag
    27961 tttagcactt aaattattag aagattgtga ctaatagaaa gatagataaa agttaacagg
    28021 agggaatata tatgtcaata tcaaaagaaa cattgcttga aatgtataag agaatgaatc
    28081 aggctagaaa atttgaagag aaagttagtt ggttttttgc aagaggtatg gtacatggaa
    28141 caactcactt atctgtaggt caagaagcat caagtgtggc agctgtaatg gctttagaaa
    28201 aaggagattt agtatcatta actcatagag gtcatagtca atttatagga atggggatag
    28261 acctaaataa aatgatggct gaactaatgg gtaaagaaac tggcttttgt aagggaaaag
    28321 gaggctcaat gcatatagca gatattgagt caggtaactt aggagcaaat ggagttgtag
    28381 gtggaggtct tacaatagca ccaggggctg cacttacaca acagtataaa aaaacaggca
    28441 aaatagtttt atgtagtttt ggtgatgggg catcaaatga aggtactttt catgaaggaa
    28501 taaatttatc atcaatatgg aaattaccaa taatatttta ctgtgaaaat aatttatatg
    28561 gtatgtcaac aagtataaaa cgtcatatga atatagaaag tattgcaact agagcagctt
    28621 cttatggaat agaggggata tcaatagatg gatataaccc aatagaggta tatgaaacag
    28681 tacaaaaagc tgctgaaaag tgtagaagag gagaagggcc tgtattaatt gaaagtagga
    28741 cttatagatg gctaggtcat tcaaaatcag atgcaaatgt gtatagaaca aaagaagaaa
    28801 tagagtcatg gaaagcaaag gaccctattg aatttttaag aaattatctt atagaaaata
    28861 atttatctaa tgaagatgaa ttagataaga ttcaagaatt tgcaaaacaa tcaatagaag
    28921 atgcagtaga gtttgctcaa aatagcccaa atccaaaaat agagtcttta ttagaggatg
    28981 tatatgcaga ctaagaatag taaaggagga aaataaaaat gagtacaagg gaattaacat
    29041 atgcacaagc aataaaggaa gcaatgtcag aagaaatgag aagagatgaa aatgtaatat
    29101 ttatgggaga agatatagga atatatggag gagcatttgg cgtatcagtt ggtatgatag
    29161 atgaatttgg accagaaaga gtgagggata ctcctatatc agaagcagct atagcaggag
    29221 cggcagcagg agcagcagcg actggtttaa gacctattat ggaagtaatg tttatggact
    29281 ttgtaacaat ttctatggac gcaattgtaa atcaggcagc aaaaatgaga tatatgtttg
    29341 gaggaaaagc tcaagttcca atggttgtta gatgtccagg tggttctgga actggttcag
    29401 cagagcaaca ttcacaaagt ttagaagctt ggttttgcca tgtaccagga gtaaaagtag
    29461 ttgcaccttc taccccagca gatgctaagg gattgttgaa agcagcgata agagataata
    29521 atccagtcat atttgttgag aataaattgt tatacagaaa aaaaggtgtt gtgccagaag
    29581 atgattatgt aattgaaatt ggaaaggcag atataaaaaa agagggtact gatgttacag
    29641 taatcacata tggaagaatg ttacaaagtg ttgaagaagc agcagagaat ctatcaaaag
    29701 aaaatataaa cgttgaaatt atagatttaa gaactttata cccattagat aaagagacta
    29761 tagtgaaaag tgtatgtaaa actggaagag ttttaatttg tcatgaagca gctaagactg
    29821 gtgggttagg tggagaaata tcagccttga taacagaaag tgaatcattt gattatttag
    29881 atgctccagt aaaaagaatt tgtggaaaag atgtgccaat accatataat ccagagttag
    29941 aaaaagcagt tgtcccaaga gttgatgaaa tagaagaagc aataaaatct ttaattgtta
    30001 gataaggagg gatagatatg gtggcaaata aagtaaaagc cacaccagca gctagaaatc
    30061 aggcaagaaa agacaatata aaattagata ggctgattgg aagtggagag aatggaagga
    30121 ttcatttagt ggatgtgtta aattatttaa aagataacaa agcaaatact actcctttag
    30181 ctagaagaat agcagaagat ttaaatatag atttagaaac tattgtaggt actggatata
    30241 atggaaagat aagaaagtgt gatgtggaaa aattaactga aaaagaaact attgtatcaa
    30301 ctaatacgtc taaatttagt gagaaaaaag agacaaagat tgaaaatgaa aattcaagta
    30361 tgtttaatac agtagaagga gtatttgaaa aaccaaatcc aatgagagcc actgtagcaa
    30421 gacgaatgtc agaaagttat tttagtgctc ctgtatttac attcaatata gaagtagacg
    30481 ctacagaatt gaaggcactt agagtaaagt taatagacac agtaaaagaa tcaactggag
    30541 taaaattaac tatgacagat ttaatagtaa tggcagtatc tagaatactt ccaaatcatc
    30601 aagcattaaa ctcagcatgg acagatgagg gtatatttag atataaagat ataaatatag
    30661 ccattgcagt tggtctagat gaaggtttat atgttccagt agtaaaaagt gtaaacaaaa
    30721 agtctttaaa agaaatagca aaagaaagca aagaacttgc cgaaaaagta aagacaggga
    30781 aacttatgcc agcagaccaa gaaggtaata catttacaat aagtaatgtc ggaatgtatg
    30841 gtataactac atttacacct ataataaata tgccttcaag tgcaatttta ggggtaggtg
    30901 ctacacaaga taagtttgtt ccagtaaatg gagaggctaa aataaaacct ataatgaact
    30961 tatcattgac atcagaccac agagtaatag atggaacagt agccgcaaaa ttcttaaaag
    31021 atttaaaaga attgttagaa aatcccttat caatgcttgt ataggttttg acatttcgtt
    31081 actgatataa atagtttgaa tagattatat tattataatt atctcagtaa atttaaaact
    31141 tttatatatt gagaattatt tttaaaggta actgtatatg aatatgattt gcttaattaa
    31201 aatatatgaa aataaatagg aggagagcct atgtcagtag aagtaataat gccaaaagca
    31261 ggtgtagcca tggaagaagg tactatagta tcttggctaa agcaagaagg cgaagaagta
    31321 aagattggag aacctatatt agaaataaca acagataaag tcaatatgga gatagagtca
    31381 gaaggagaag gtacacttgc tgcaatcata cataaagagg aaggtgaagt attaccagta
    31441 tttacagtaa taggtgtaat agcagaaaaa ggagaaaatc aagaggaagt aaaagctaaa
    31501 tatttatctg gaaatgtttc aaaagaagat atagttgaag agaatcaaaa catagaagct
    31561 aaagaagaaa aaataaacaa aaaagagtgt aatcatgatt atgatgtagt ggtaataggt
    31621 ggaggacctg ggggatattt atcagcatta aaagcagctc ttttaggtgg aagagttgct
    31681 ttagttgaag aaaatatact aggaggtact tgtttaaata gaggttgtat ccctacaaag
    31741 acttatataa aaacggcaga aatattagaa gaaattgacc agttatcaaa gagaggcgta
    31801 aaagtaactg tagataaaga acaggacatt aagaaggcta taaaatataa aaatagagta
    31861 gttaaaaaac ttacagctgg tgttggtgga ctattaaaaa gtagagatgt agaggtgttt
    31921 aatcttaaag ctagtgttaa ggaagagcat aaagtaattt tatcagatgg aaaagtttta
    31981 gatacagaaa atataataat tgctacaggt tctaaggtaa gaatattacc tataaaagga
    32041 atagaatcaa atttaataat aactagtaca gaagctttag atttagaaac agtaccagaa
    32101 gaattagtaa taattggtgg tggagtgatt ggttgtgaat ttgcagagat attcaattca
    32161 agaggctcta aagtaactat agtagaaatg gaagatagag taattccaaa aatggataaa
    32221 gatttaagtg aatctttaaa atattctctt ggtaaaaaag gtataaacgt gctaactaaa
    32281 aagaaagttt ctgaatttaa agaagaagga aataacatat tagtgtgtat tgaagatgaa
    32341 gaaccaataa aagcagattt atgtctatat gctataggaa gagaagctaa tctttctggt
    32401 atagaggact tagatattaa aatagataaa ggttcaatag ttgtaaatag caaaatggaa
    32461 acaagtatac caagcatata tgcagtaggt gatgtgactg gaggtgtaat gttagctcat
    32521 gcagctttta aaatgggaga ggtagcagct tcaaatgctc taggtatgaa taaagaagtt
    32581 gatttaggtg cattacctag ttgtgtttat acaattccag aagtagcatc agttggtatt
    32641 acagaagagg atgctagaaa aaaatataat gtaaaagttg gtaagtttaa ttttgctgga
    32701 aatggaagag ccttagcttc tggtcaagaa caaggatatg tgaaagtagt agcagatgct
    32761 aaatatggag aaattttagg tatacatatg tttggatgtg gtgtagcaga acttataaat
    32821 catgcagcat cattcaaagc attagagata cctgctgatg aagctagtga gttaattttt
    32881 ggtcatccat gcacttcaga agctttgatg gaggctttag cagatgtaaa tggagaatgt
    32941 ttacacttac ctaaaaaata aattgttaat gtaagtctat tattaatgtt aatttatatc
    33001 ataaagtttt aaaagaatta cttagccata ccctttgttg tacaaataaa ttatttgcac
    33061 aacaaagggt attttttatg gaaaaatata tagtatgaaa cctttgtagt ttttgttata
    33121 caaaaaagat agtaatataa aataacagta ttattttaga atgatttcaa ttgtaattag
    33181 aaaaaatgat ttttttgttt ttaagaaaag tattatttgt aaaatgtaaa aaaaattttc
    33241 ttaatatttc aataaaagaa tagcttgatt taaatgttat tactttataa aaaaataata
    33301 agcacaaaaa aaacaatata tggcatataa gttgctatat aaataataag gagggaacaa
    33361 ttataaatga ggcaaagaat aaaaaactta attgaaaatg aagataaaaa aaatccttta
    33421 acagatgaag cgatttcaag tatgttaaat attcgaagag aagatgttac ctttttaaga
    33481 aatgagttaa aaatagaaga ttcaaggcaa agaagaaaag ttgttttaat taaagctata
    33541 aaagacattt taaaagaaga aaaaactata aataaaaatg atataacaag aagattaaac
    33601 agcattggtt ttacagtatc aagatttaca gttatacagt acttaaaaga gcttcaagat
    33661 tctgataact taaaacttta taaagaaagt gatgataaaa aagaagaaat aaacaataat
    33721 agagaaaaca cagatatagc atttgataaa ctcataggta gtagtggtag cttaagtaca
    33781 attataaaac tagctaaagc agcaattcta tatccacctc atggtcttca tactatcatt
    33841 atgggaccaa ctggtgttgg aaagagtgag ttagcagagt gtatgtataa atttgcacta
    33901 gaatcatcca gattccctaa gaatgcacca tttatagttt ttaatgcagc agattattca
    33961 gagaatccac agcttttgtt atcacagtta tttggacatg ttaaaggtgc ttttacagga
    34021 gctgacgaga gtaaagaagg attagtatca aaggcagatg gaggaatact gtttatagat
    34081 gaaatacata gattacctca tgaaggtcaa gaaatactat ttcaattgat tgataagggg
    34141 atggtcagaa aattaggaga aacaaaacta acaaataaaa tagatgtaat gattatttca
    34201 gcaacttctg agccaataga ttctcattta cttaatacat ttaaaagacg tatacctgta
    34261 acaattgaaa taccagaatt agttgcaaga ccattaaatg agaggtttga tattataaat
    34321 aatttcttct taatagaagc tcaaagaatg ggagcaaaca tacatataaa gtcagatgta
    34381 ttaaaagctc ttatgcttta cgactgtata ggtaatgttg gtcaactgag aagtgatatt
    34441 caagtagctt gtgctagggg actactaaat caacttacaa ataaacaaaa agaagtaaac
    34501 ataagcatat cagatgtacc tggatatgtt aaacaaggtc ttataaaaat cagaaattgc
    34561 agaggtaaaa tagaaaatta tgtagatgga gacttaatta tagactcaaa tcttcaaggt
    34621 gaaatagaaa agaaaaatga agacatatat acttttccag atgaaatata taaacttact
    34681 gaaagaagac atattgattt attggaacaa ggtttagatt ttgatgttat aaatagaatc
    34741 gttggaggag aattagaaag ttgcattcaa aaatacataa agcaagttaa aaaagtttct
    34801 attagtaata atcttccaga tataagtccg attgttggaa aatatatagt agagctaact
    34861 tatgaagtaa tcaaaatagc atcacaatat cttaattcat tagaaccaaa tctacattta
    34921 tgtttagcag tacacttaaa agccgctttt gagagattaa aagaagggaa agtaatatca
    34981 aatgctcatt tgaaagaaat aataggaaat tatccactag aatattctgt agcagaaaag
    35041 gtggctcaat atctaaagga aagatataac ataaatttgc caaaagaaga aataggtata
    35101 atagcaatat attttaaaat gagtagcaga agaaatatat ctgaacaaaa aaagataggt
    35161 gtacttctta tggctcatgg aagggtagca tcttctgtat gtgaggttac aaataaacta
    35221 ttaggaatta atcatgcaag gtatttagat atgccatttg agaagaaacc agaagaaatg
    35281 attgaagaag ctattacaat aataaaagat attgatgaag gtagaggtgt aattatacta
    35341 gtagatatgg gttcactttc atattttgga gatattattt ctggaaagac tgggattgat
    35401 ataagaacaa tatctagatt tgatacattg ctttctatag aagttatacg aagagcagtt
    35461 ttaccagatt taacaataga cgagatagtt gatgaagtta aaggtataga gccacaagta
    35521 aaagaaaaag ttagtaaata taacaataca aaagtaaatt ttagaaaacc tataatttta
    35581 acattatgta ttactggatt agggggagct tgtaagataa aaaaaatcct agaggaaaat
    35641 attaaaagta taactgaaaa ggttgatata gagcctatag gtatgattga atatccaagt
    35701 cttgatttag agataaaaca aataagtaat gataaaaatg taattgccat agttggaaca
    35761 attgaccctc agatagcaaa tatccctttt tttagcctag tagatataaa tgataaagta
    35821 aagttaaatc aaataaacaa tctaattgat acaaatcaaa ttagtgagac aaataaaaaa
    35881 tatgaattat cagatttaat acataaagaa gatatattcg tagatttaca agttaaatcc
    35941 aaagaagaag ttctaaaata tatgagtaaa aaacttagag agaaaggtta cgtgacaaaa
    36001 aatttctata aaaaagtagt ggaaagagat aacttatttc caactgaatt atcagatgaa
    36061 atagcaatac ctcacacaga tggaatagat gtaattagac cagctataag catagctatt
    36121 ttaaagaatc ctattgtgtg gagtaaaaat aaagtaaaaa taatactttt attagctgta
    36181 gaccaaaagt gttttaatcc attaagcact ctactttcat ttatagaaac agatgaattt
    36241 aagcagataa aaaacataaa agatagagat tttattaggg agatgatatt agatggagtc
    36301 aatgaaaata attagtagta atttaatttt taaaaatatt gaagtgtcta acaatgaaga
    36361 tgcattaaag tttttgggac aacgtttatt tgatgaacag tatgtaaagg caagttacat
    36421 acaagctgtt atagcaagag agaaaaagta tgctacagga ttaccaacag aaatatatgg
    36481 agtggcaata ccacatacag atatagtaca tgtaaatgag ccaggaatag caattgggat
    36541 actgaataaa cctgtaaaat ttataatgat gggtacagat gatacagaga ttgatgtaaa
    36601 agttgtattt atgttagccg tgaaagagcc acaagaacaa ttgcaactat tagaaaaact
    36661 tatgactata tttcaagata agaatatact aaataatatt gttgacttaa gcgaagaaag
    36721 tgtgagtgat ttattaaact ctaaattgaa aaattgaata tataaagtat attaaaaaat
    36781 aaaaatgtta ttgaagattt ctaactatag gtatctttaa aataaaataa tttcaatggg
    36841 ggtgactaat gtgactaaaa agatattagt tgcatgtgga acaggagtat gtacttcaac
    36901 aattgctata aacaaattaa agaaagcgct tcaagatatt ggtaaattgg atatggttaa
    36961 tattacacaa tgtaaagtgg tagaagttgc atcaaaagca cctgattatg atttaataat
    37021 ttgtactaca caagtatcta gtagtataaa aacaccagtg ataaatggat tacctttttt
    37081 aactggtgtc ggaatggata aattaatcaa ggatgtatta gaaaagctag aattataatc
    37141 aaaaaaatta aggaacatgg tctctaaaat tataagtttt agagacttaa aattaaataa
    37201 tggagggtct ttatatggag atattaaatt tcattgttgg tctaggcgca caagtaatga
    37261 tgccgataat aatatgtatt tttggtttga tattaggtac aaaactagga aaatctttaa
    37321 gagctggttt aactgtagga gttgggttta taggtcttaa tactattata gctttgctaa
    37381 cagataattt aggtccagct acacaacaaa tggttaagaa tttaggacta agtttatcca
    37441 taatagatgt aggatggcca gcagcatcgg ccatagcttt tgcatcaaca gtaggggctt
    37501 taattattcc aataggtctt gtggtaaata taattatgtt aattactaat accactcaaa
    37561 ctgttgatgt agatatatgg gactactggc attttgcttt tacaggagct ttagtggcag
    37621 gggctactca aagtgtaatg tggggagtat ttgcagcagt agcaaatatg ataattgtat
    37681 tggtaatggc agatttaact gctccaggga tagaggaata tttaggtatg ccaggtatat
    37741 ctcttcctca tggatttaca caagcatttg ttccaattgc tatagtagta aataaattat
    37801 tagacctaat accaggcata aataagattg agataaatgc tgatacattg cagaaaaagt
    37861 ttggattatt tggagaacct ttaataatgg ggtctgtaat aggggtaata attggtgttg
    37921 cagcgaaata tgatataaaa ggaatattac aattaggtgt aactatggga gcagtattaa
    37981 tattaattcc aaagatggca gcgttattaa tggaaggatt attaccagta tcagaatctg
    38041 cacaggaatt tattgaaaag agatttaaaa atagaggtaa gatttacata ggcttagatt
    38101 cagcagtagg aataggtcat cctgttacac tatcagttgc attagtgttg gttcctttga
    38161 ctatacttat agcagctata ttgccaggaa ataaagtttt gccatttgca gatttagcag
    38221 taattccatt tgcacttgta ttaattgtgc caattacaaa gggaaatgtg tttagaacat
    38281 taataatagg aataattata attacttctg ggttattaat cgcaactaat ttagctccat
    38341 tatttactca aatggcttta aatgctagtt ttaaaatgcc tgaaggagca actatgattt
    38401 caagtatttg tgatggtgcg aatccattaa gttgggtatt tgtaaaagtt atgaactata
    38461 aagttattgg aggagtagtt tttggagtca tagctttagg aatggctatt tacaatagga
    38521 atagaataat aaatgaaaat aaaaagctat ttatagaaga ataaggttaa aacaatttta
    38581 ttaaggagtg tataagttat gaaaaaatat aaatatgatt ctaaaaggta cttacttagc
    38641 gttacaatac caggattttt tatgatttta atattgattt atgcactgtt taataactat
    38701 ataaatttta atttgaatat atatagtttt ttaataattg tttgtactta tggtatattc
    38761 aatactttca tatcatcaag taatcctaaa aaaattatta ttgataacaa atgtatacat
    38821 ttttcttcat ttatgactac acataagtat gaaattaaag atatagaaaa gataagaata
    38881 agagaatttt ataataaaaa aatttatcta aaaattaatg aaggaaacct ttttaaaggt
    38941 aggtattgga taagaacaaa tatgtttaat aatagtatag aattatatag ctatttaatt
    39001 gagttggaag agtgcttata tccagattca ttaaagttta gaaataaaag atttgagaat
    39061 aagaacatat agaaataaaa aaattaaatt gaagcctagt ttaatatgtt ttgatagaat
    39121 atgagtttga gcttcatgct gaatttttag aattttcaat taaaataata atataatgtc
    39181 taagcaatta gagacgtata aggcatgtat ataatgaatg ggaggattta attaatgtta
    39241 ttagacaaat taagaaaaga agtattacaa gcatctttag accttttaaa ttataattta
    39301 gtgacattaa caggaggaaa tgtaagtggt agagatgaac aaactggcta tatagcaatc
    39361 accccaagtg gaatggatta tagaaatctt ataccaagtg atattgttat agtagatgta
    39421 gatggaaatg ttatagatgg aaaatggaaa gcatcagtag atttaccaga ccacttgtat
    39481 atatataaac atagagaaga cataaatagt ataattcata cacattctac atactcaagt
    39541 tgttttgcta ttttaaatga acctatagaa tgtgcaagta caactcttgc aaatgaagta
    39601 ggaggttcag taccagttgc aaaattttca ccaccaacgt ctaataagat gggaaaatgt
    39661 gttattgaag caattggtga caaaagagca tgtcttttag caaatcatgg tgtaattgca
    39721 gttggtccat ctgtaggaca cgctcttaca gctgctgtaa tgttagaaga ttcagcaaaa
    39781 gtatattact tagcaaaatc aattggcaca ccagtgctac tgcctgatga agaaattcaa
    39841 agagctagag atgtattttt taatgtctat ggtcaggata aataaacgag ataaaatttt
    39901 aaaagtaata ttaaaatata gatattcaat gatattaagg agatttataa tgatataata
    39961 agtttaatag ttaagaagta aaatatgaaa tgtaatcgta tataaattca cttaagacta
    40021 acaaataata agataaaata aaaaaggagt ctgtatttct atgtattact tattaaacaa
    40081 aagcacaaat ccttacttta atttagcatt agaagaatat ctgtttttaa atgataaata
    40141 taatgatgat attataatca tatggaggaa tgaagaatcc atttttatag gtaaaaatca
    40201 aaatccatat caggaagtac atcatgatgt tattgaaaag ggcgaaattc ctattttaag
    40261 aagaatatct ggaggaggca ctgtttatca tgatttaggt aatattaata tgtcattcat
    40321 acaaaaagat agacaacttc atgagataga ttttttggag catactaagt ttatgcaaaa
    40381 gatgctctta accttgggat taggtgtatc tataactgaa agaaaggatt tatttttaaa
    40441 tgggaaaaaa atttcaggct ctgcacagtc tataaaaaga aaaaattctc tttatcatgg
    40501 aactcttctt tacgattcag atttaaataa attgactaag tatttaaact cagataaatc
    40561 aactgaatct aatgcaacta agtctgtttc aagtaaagtt acaaatatta agtttttgct
    40621 cgaaaaaaat ataagttatt ttttagatta ttgtgttgaa tatttaaaat ctaatataaa
    40681 aaatataaaa gaacttgaat tagatgaaga agatattaaa aatgtataca atttagtaga
    40741 agataagtat aaaaagatag agtggacata tggtaaaacg cctaaatttc aagtggtgct
    40801 accatttaat aataaaagta aaattaatat acatgttaat agatggaaag tatcaaaatt
    40861 ttttatttct tatgatgata atgtaattaa tatggacaag ttgttggatt taaatttctt
    40921 taaagaagaa ataaaagaga gtattttaga aaattatcca cattacatag atttaattaa
    40981 tttaatattt taatataaaa cc
//
LOCUS       DAFNNB010000017        40834 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0017, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000017 DAFNNB010000000
VERSION     DAFNNB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 40834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40834
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0017"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            65..1468
                     /locus_tag="L3M60_000795"
     CDS             65..1468
                     /locus_tag="L3M60_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225489.1"
                     /translation="MKGSDLVKFELMDFLMNPFVLMFAAVITGILFGKIKFGKFNFGV
                     SGALFTGLFIGWLAYSLGNLIIAKGETAAGYKAATVMMGNGIISPDFFDFFLIIFVAA
                     VGLLAAKDMKAVLKKYGARFVILGVLITFIGGSMTYAMTLLSSDKGSSAYEVSGVYTG
                     ALTSSPGLAAALETAGKHAEDVSKEFEKASTKDKEEILKVVDPEGKLDVSTTTSLTQE
                     QIDKYIAYAEAGVGIGHAVAYPFGVLIVILGVNFLPKLFRMDLKEERRKYEKEMKEAR
                     DSVSGKTIPEVPFNIITFFLTCLAGYLVGGIHVFMGPLGYFTLGATGGSLIVSLVLGY
                     IGKIGVINFRMEEKVLNILKQIGLVFFLAIVGLRYGGKVVDSIMTSGMHLALVAIAVG
                     VTAMMIGFLVGKYVFKLNWILLSGAVCGGMTSTPGLGAAVDALDSDDPAAGYGATYPF
                     ALLTKVILVIVLHKLPM"
     gene            1495..3039
                     /locus_tag="L3M60_000796"
     CDS             1495..3039
                     /locus_tag="L3M60_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBN6225490.1"
                     /translation="MNNEGTFEELSGRLRNKKLCDLIMNADEAAKFIKDGMVVGVSGF
                     TPSGYPKAVPLAVSERAKSGEKIKLTVYSGASLGPEVDGAWSEAGIIERRLPYQTNSI
                     LRNNINKGVVDYIDMHLSHSTQFLNYGTIPKVDVAIVEALAITEDGNIIPTSGIGNSP
                     SFIKSADKVIVEINLAKPMEMEGMADIYITENPPNRKPIEINHPKDRIGTTYIPCGLD
                     KIAGIVITNMQDKTRPLGVVDEASKKISNNIIAFLREEVKAGRLSKNLLPLQSGVGSV
                     ANAVLYGLCESEFENLTCYTEVVQDSMLDLIRMGKVTMASTTSVSPSPEGLIKFEKDI
                     DFFKDKIILRPQEISNNPEIARRIGVIAMNTAIEVDIYGNVNSTHIMGSKMMNGIGGS
                     GDFARNGAITIFSTESIAKNGDISSIVPMVSHVDHTEHDVMVIVTEQGYADLRGLAPR
                     ERAIKIIENCAHPDYKEQLRDYLNRACKSGAKQTPHILDEALSWHSKFMSTGTMKKAE
                     TFKSAL"
     gene            3070..4461
                     /locus_tag="L3M60_000797"
     CDS             3070..4461
                     /locus_tag="L3M60_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HBN6225491.1"
                     /translation="MGKAVENFEDLSKEYINGYIERARKAQREFECYTQEQVDKIVKI
                     VGKVVYYNAEYLAKLAVEETGMGVYEDKVAKNKSKAKVIYNNLKDKKSVGIIDIDRET
                     GITKIAKPVGVVAAITPCTNPIVTPMSNAMFALKGRNAIIITPHHKAIGCSTKTVEMI
                     NEELEKIGAPENLIQILDQQSRENTRNLISSADVVIATGGMGMVKAAYSSGKPALGVG
                     AGNVQCIIDRDVDIKEAVPKIIAGRIFDNGIICSGEQSVIVAEEMFDKIMDEFKNNKG
                     FIVRDKAQKEAFRNAMFVNKSMNKDAVGQSVHTIAKIAGVEIPEDTKIIVIEADGPGE
                     EDIIAKEKMCPVISAYKYKSFEEGVAIAKANLNVEGKGHSVSIHSNTVKNIEYAGENI
                     EVSRFVINQCCATSAGGSFFNGLAPTNTLGCGSWGNNSISENLDYKHLINISRIAYYM
                     PENEVPTDEELWG"
     gene            4654..6150
                     /gene="abfD"
                     /locus_tag="L3M60_000798"
     CDS             4654..6150
                     /gene="abfD"
                     /locus_tag="L3M60_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyryl-CoA
                     dehydratase/vinylacetyl-CoA-Delta-isomerase"
                     /protein_id="HBN6225492.1"
                     /translation="MALMTGAQYIESLRKLNTKVYMFGEEVKNWVDHPMIRPSINCVA
                     ATYDLAHDPEYADLMTVTSNITGEKINRFGHLHQSTDDLIKKVKMQRLCGQKTASCFQ
                     RCVGMDAFNAVYSTTFECDKAHGTNYHDNFVKYLTYIQENDLVVDGAMTDPKGDRSLS
                     PSAQPDPDMFLHIVERREDGIIVRGAKAHQTGSINSHEHLIMPTISMTEADKDYAVSF
                     AVPSDAEGVFMIYGRQSCDTRKLEEGADVDLGNKEFGGQEALVVFDNVFVPNDRIFLC
                     GEWDFSGMLVERFAGYHRQSYGGCKVGVGDVIIGAAALAADYNGANKASHIKDKLIEM
                     THLNESLYCCGIACSSEGHKTEAGNYQIDLLLANVCKQNVTRFPYEIVRLAEDIAGGL
                     MVTMPSEKDFKSDLKVGTSGMTIGEVCNKYFKASSVASTEERMRILRFLENICLGSSA
                     VGYRTESMHGAGSPQAQRIMISRQGNINQKKELAKTIAGIKKEEALNL"
     gene            6285..6560
                     /locus_tag="L3M60_000799"
     CDS             6285..6560
                     /locus_tag="L3M60_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225493.1"
                     /translation="MTCGVRFCGGCNPRYERGKALAIIKEHFNGRVDFSIAQENLEYD
                     LLLIIGGCATLCASYQQYKVKNECVKMWDFSHIERVINVIESAICET"
     gene            6584..7891
                     /locus_tag="L3M60_000800"
     CDS             6584..7891
                     /locus_tag="L3M60_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase family protein"
                     /protein_id="HBN6225494.1"
                     /translation="MSWRELYQSKLCSATEAVKQIKNGDTVVFAHCVGEPPALVEAMI
                     ENAEQYKDVEIKHMVSLGSGGYTAKGMEAHFRVNPMFVSGNVRKAIENGDGDFTPTFF
                     HEVPKLLREKRLKCDVVLAQVTPPDEHGYCSLGTSVDYTYEAIKTARTVIVQVNDQFP
                     RTYGEVVHVSDFDYIVEKSQPLYELEPAKIGEVEEAIGKNCASLIEDGSTLQLGIGGI
                     PDAVMLFLTDKKDLGIHSEMISDGTLALYEKGVINGKYKNFDKEKMTVTFLMGTKKLY
                     DFVNNNPAVEVKPVDYVNHPAIIMKQHKMVSINSAIQVDLMGQVVAEAMGLRQFSGVG
                     GQVDFIRGVSMGEDGKAIIAMPSITTKKDGTVISKIVSIVDEGAPITTSRNDVDYIVT
                     EYGIAELKGKSLRERARNLINIAHPSVRESLAIEFEKRFKEKY"
     gene            7911..9029
                     /locus_tag="L3M60_000801"
     CDS             7911..9029
                     /locus_tag="L3M60_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybutyrate dehydrogenase"
                     /protein_id="HBN6225495.1"
                     /translation="MEALRVVPKIFYFDTFKEFNEEFKIGKNDLVITNEFIYEPYMKP
                     LGIDTNLIFQEKFGTGEPSDEMIDSMTKEMKKYNFDRIIAFGGGTIVDICKILALDVP
                     EKSIDLFEGEVPPKKIKELVVVPTTCGTGSEVTNVAIAELKSKHTKKGLAVEETYADY
                     AVLIPETVKGLPYKFFVTSSVDALIHAIESYLSPKASPFTEMYSLQAIKMIMDGYKKI
                     VDKGEEERFNHLRDFVLASNYAGIAFGNAGCAAVHALSYSIGGAFHVAHGEANYQFFT
                     EVFKMYSRKKPNGKIIKCTKILADALECDPNCDVYGELEKFLNKLIAKKALREYGMVE
                     AQIDEFTDSTIANQQRLLANNYVELNREEIREIFANLY"
     gene            9314..10234
                     /locus_tag="L3M60_000802"
     CDS             9314..10234
                     /locus_tag="L3M60_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HBN6225496.1"
                     /translation="MYKSENFSKKSVLINSFLSKFLKIIGWGLVSLFTLSALVLSTIS
                     IIIGWAVILPIQVCAVFLIIGGVCIFKGKRLGDQISRYKKYCTIINNRNIIPVELIAE
                     NTSKSLNFIIKDVQKMIDKNYFINTYIDKRNNQIVLTNEDFVPTRYEDVAYEVKEEEK
                     TDEDNEVDTIINKGMNYLTHIKEANKNIKSESMCDKIVQVEDVTSKIFDVVKHDPSKL
                     TQIQKFMDYYLPTTLKLLNSYHTLEEQGIDRENITTTMESIENTMNTIVVAFENQLDY
                     LFEDEAIDISTDITVLENMLVQEGLTSGKF"
     gene            10349..11506
                     /locus_tag="L3M60_000803"
     CDS             10349..11506
                     /locus_tag="L3M60_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBN6225497.1"
                     /translation="MNNLDDIPVMPTLTLDPFGEESNTSDIDNSDLLMKKDEKDPEEE
                     KLSESERKMVKEFAEKIDITNTNMILQYGAGAQKKVASFSETALKSVKTRDLGEVGDM
                     LTNLVTDLKAFSADEKEQSGFLGIFKKANNKISNLKTKYDSAEVNVDKVSKELQKHQV
                     KILKDIAMLDKMYELNLAYSKELTMYIIAGKQKLKDMKEYEMPKLREKARLSGSTEDA
                     QSVNDMVSLCDRFEKKIHDLELTRMVSLQMAPQIRLVQNSNNLMAEKIQSTIVNTIPL
                     WKNQIVLALGISHLNQAMKAQREVSNMTNELLLKNAETLKMGTIETAKESERGIVDID
                     TIKKTNQSLISTIDEVVKIQHDGRIKRQEAEVELSKIENELKSKLLEFTVK"
     gene            complement(11594..13093)
                     /locus_tag="L3M60_000804"
     CDS             complement(11594..13093)
                     /locus_tag="L3M60_000804"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBN6225498.1"
                     /translation="MAFYFDTPSHTFSEYLLVPGFSSTDCIPANVSLKTPVTKFKKGE
                     EADIYMNIPLTSAIMQSVSDDKMAIALAKEGGISFIYGSQTIENEAAMVARVKSHKAG
                     FVVSDSNIKPDNTLKDILDLKEKTGHSTVAVTEDGTSTGKLLGIVASRDYRISRMDLD
                     TKVSEFMTPMSSIVYANKDVTLKEANNIIWDHKLNSLPVLDDNGNLMYMVFRKDYSSH
                     KENPLELLDSSKRYVVGAGINTRDFAERVPALVEAGADVLCIDSSEGFSEWQKITLDF
                     IREKYGDTVKVGAGNVVDREGFLYLAEAGADFVKVGIGGGSICITREQKGIGRGQATS
                     IIEVAQARDEYFEKTGIYIPICSDGGIVYDHHITLALAMGADFIMLGRYFSRFDESPT
                     NKVNINGSYMKEYWGEGSARARNWQRYDLGGDKKLSFEEGVDSYVPYAGSLKDNVTLS
                     LSKVRSTMCNCGALSIPELQKNAKLTLVSSTSIVEGGAHDVMLKDNRNL"
     gene            13688..15112
                     /locus_tag="L3M60_000805"
     CDS             13688..15112
                     /locus_tag="L3M60_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBN6225499.1"
                     /translation="MSSSNQSTIKTSIQRVGKFLSGMVMPNIGAFIAWGLITALFIPT
                     GWMPNEKLSTIGDPMIKYLLPLLIAYTGGKAIAGQRGGVIGAAAAMGVIVGADIPMFI
                     GAMIMGPFAGWIIKKFDKFVDGKIPTGFEMLVNNFSIGIIGMLLAILGFYAIGPAIVA
                     GTALIESGVQFIVSKSLLPLVSVFIEPGKVLFLNNAINHGILGPIGIAEAKEAGKSIM
                     FLLESNPGPGLGVLLAYWMFSRGSVKQSAPGAVIIHFFGGIHEIYFPYILMNPVLILA
                     TIAGGAAGILTFSILGAGLVAAPSPGSIFALMALAPKGGLLPVLAGVAVATVVSFLVA
                     APFVKRASANQSEEDNTSLEEAKAKVSDMKSVSKNSKKDIEEKQLEVNEIKKIVFACD
                     AGMGSSAMGASRFKNRIKNLDLNIEITNSSVDNLPDDTQIVVTHNTLVERVAKNNSSV
                     EIVSINNFLNDPNLDVLFKRLESK"
     gene            15146..17275
                     /locus_tag="L3M60_000806"
     CDS             15146..17275
                     /locus_tag="L3M60_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HBN6225500.1"
                     /translation="MKKKKITSRQKKIILMIVENSKKNIPITISEIAGMLELSSRTVL
                     RDMSGIEKWFDENDFNFVKKPGVGLILEESIENQNFIIELLEEEKIEKEYSKEERNLI
                     ILSKLLVSNEPVKSYYFAKILKVSEGVLNNDFALASKWLERFDIELVRKPGLGVYLKG
                     QEKSFREAYVNLIYDSFNEKEILDMVRNISENIQTDKTIEILSENRLLNLMDKCIIRK
                     VEGTLTKKLSDLDVNLADSAYIGLVVHISLALQRIKNGENITMDKEFLKELSITEEFK
                     LAKEIVKGMEIDFSMDIPLDEVGYITMHIRGAKQRSSSNHNALNLDDIEVMDITNKMI
                     DLAEDEFKISLKNDERLFKDLANHLGPSINRLNMGLEIRNPLLDEIKSKYSYVYNGVE
                     KISRIIKDKLSINSIPESEIGYIAMHFASAIEKNLMMYANINIVVACPTGIGTSRFLS
                     TKIENKFPNLNILETISAINIDEEYLKEKDVDLIVSTVELNTSLNYICVGPFMSLDDE
                     QLIKEKIKSIAQNKLINLNVKNGTKNKNKIYAQITESMNIGKDILQFLEEIRFEKFES
                     KDLSELIEDSSKIFAKSSEDIISIKESLKERLKISIPYIEESKILLLHCISERIDIMK
                     LSIIKLENRIILDSNEEIDNVVFMLLPKNSPSYQRQIMSEISGSLIDNIIFTNKINKF
                     SIEEMILEVKDIVFNFYTNRLKAFIDK"
     gene            17305..17727
                     /locus_tag="L3M60_000807"
     CDS             17305..17727
                     /locus_tag="L3M60_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225501.1"
                     /translation="MNKVLNENNIFLGLDSVSKEEAITLAGRKLVENGYVKEEYIPAM
                     LEREKVMTTYMGMGVAIPHGVNEAKKEILSSGIVILQFPNGIDFDGEKAYLLIGIAGV
                     GDEHLEILSNIAVVLDDDLTERLKNSNDKQAFMEAFAN"
     gene            17746..18897
                     /locus_tag="L3M60_000808"
     CDS             17746..18897
                     /locus_tag="L3M60_000808"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBN6225502.1"
                     /translation="MKKAIQFGAGNIGRGFIGGLLVKSGYHVVFADVNEEILNSINKD
                     KKYTIHIRDVECIDEVIDNISAVSSIKEEIIDEIVEAEIITTAVGPLVLTKIASTIAK
                     GIKNRKEKGLTSNLNIIACENAIYASSSLKEEVLKYLNKEEVEYLEMYIGFPNCSVDR
                     IVPPGKNENPLDVTVENFYEWNVEKQGFKGEIPTIVGMNLADNLMAYIERKLFTLNTG
                     HAITAYIGYLKGYKTIEESIKDKFIYDIVKSAMVESGEGLIKKYNFDSEAHYKYIDKI
                     LNRFKNPYLNDDVLRVGREPLRKLSDKDRLIKPLMTAKSYDLSVDNLILGIGAALHYN
                     NSEDAQSVELQELIKSNGVKKAVAKIANINNDEELLNNIEKSYIFMENL"
     regulatory      19003..19104
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            19287..19859
                     /locus_tag="L3M60_000809"
     CDS             19287..19859
                     /locus_tag="L3M60_000809"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBN6225503.1"
                     /translation="MKALQEKILREGSVSGNDILKVDSFLNHQIDVAFLNEIGREFKE
                     RFEGEKVDKIFTIEASGIAIASIVSQYFDNAPVVFAKKSESKNLDKDVYETNVYSFTK
                     AREYSVKVSKKYINKGENILIVDDFLANGRAALGLKDLIEQAEANLVGVGIVIEKGFQ
                     AGGALLKANNVRLESLAVVESIDNGTVKFR"
     gene            19923..20573
                     /gene="fsa"
                     /locus_tag="L3M60_000810"
     CDS             19923..20573
                     /gene="fsa"
                     /locus_tag="L3M60_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBN6225504.1"
                     /translation="MRFFIDTANIEEIKEANDLGVICGVTTNPSLIAKEGRDFIEVVK
                     EISEIVDGPISAEVISLEHKGMIEEAEKLSKIHKNIVIKIPMTAEGLKAVKVLSSKGI
                     KTNVTLIFSAGQALLAARAGATYVSPFVGRLDDISQNGLDLIEEIVDIFSVNSIEAQI
                     IVASVRNPIHVLQAARMGADIATVPLKVINQMIKHPLTDKGIDSFMKDWEGASLKL"
     gene            20725..23649
                     /locus_tag="L3M60_000811"
     CDS             20725..23649
                     /locus_tag="L3M60_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6225505.1"
                     /translation="MDKILREIQREDKKNPYTDQELAEMLNVARSEVISVRKKNNILD
                     SRERRKKILIKDISKIVSENPQMSERKITEILIENGYNISRSAVSKLLKEENLNQSKS
                     GIKDKDLASSGKYVKVSKVVEEDGFEELIGIKGSLKEKVNLAKSAIMYPPNGLHTIIY
                     GETGVGKSELATCMYKYAIKNNIKEENSPFIVFNCADYAENPNLLIAQLFGVVKGAYT
                     GADANREGLVEQANNGILFLDEIHRLPAAGQEILFSLIDRGEFRRLGESSSNRKANVL
                     IISATTENPDSNLLQTFRRRIPMVINLPNMSERPKSERYEIIIKFFEREAKRVNKNFI
                     ITKEVMCALMNYKCTGNIGQLKSDIQVTCARAFSKSIFSSDKVIIDLDSLRDYIKSGY
                     YDCNCENEKYSEFSVEDIYIDISELVSGKNRILSDNEISEIYNYAEKELKVLESKCFS
                     EEELKNAFIEKLDKKFNAIKNNKNLSDRRSQIDWGGQLKESTLEIMDKVIVFIKEQFK
                     HVNQGLYLALAIHIEHAIGRIKDGKTIINPSLDKIKASMPMEYELSRYIMGIVEDLTQ
                     VTFPEDELGYLAYYINKFCYNEESIKDKVKVVIVTHGKVGIEMSKVVNHILGIECTIG
                     IEIALTDSPSEGIEHVLEELQKIEARKGILVLIDMGSLVILGDEVEKRLGIRCKTVNR
                     VDTLLAMEAGKLATIEGKSLDGIIADLKKNKNYAMINTNKFSYRKNEYGKKNVIITLC
                     LSGVGTALNLKDHIEKQIKEYDASIEVKPIAFLNNNLEDELNDIEAEYNIIAICGTID
                     IDYKNVPFISYNEVLGKNGINKVLEHLNSKQPALNIENQLNNLIHEDLILYNFEGISK
                     EYIIDTLVSKLEEGGYVDSKYILSVYKREAMGSVVMASKVAVPHGLPENVIKPAIAIA
                     RLNKPIVWDNKFMVDLVVLLALKENNKKEIRSLFSKINDQHTLEVLLNTEDKNEIKEL
                     LS"
     gene            24238..24696
                     /locus_tag="L3M60_000812"
     CDS             24238..24696
                     /locus_tag="L3M60_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225506.1"
                     /translation="MEFINFVSDKVIELKMDCKTKENFFKEIHNKVFDLGFVKEEFGE
                     KILERENVFPTGLNLGDYGVAIPHTDAEYIKEQFIAVCTFNEPVVFSSMEDQDEKVPV
                     NLAFVLGLNQPHSQLSVLTELMGIMQNKELVDKLMSSTDKEEVLKTLKSL"
     gene            24749..25024
                     /locus_tag="L3M60_000813"
     CDS             24749..25024
                     /locus_tag="L3M60_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225507.1"
                     /translation="MKKILVACGAGIATSTVVCDKVEKLVKENNIQAEVIQCKIAECA
                     TKQEGADLIVSTTILPTTYNIPAIKATAYITGVNTAALDKKILDALK"
     gene            25083..26357
                     /locus_tag="L3M60_000814"
     CDS             25083..26357
                     /locus_tag="L3M60_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012951111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBN6225508.1"
                     /translation="MDKLLAVVQYILNMGPTVILPITIVVLGMIFRIKFTKALKSGLT
                     IGIGFVGINLVVNLLTSTLGPAAQGMVERFGLNLTVIDVGWPTTASATWASPVAPILI
                     PVCLLVNLLLLYFNKTKILNVDIWNYWHFIVAGAVGYIVTGSYVWAIFCAILMELLVL
                     FIAEKTAKNVQEFYDLEGIALPTGSTASFAPLGYLVGKLVEKIPGINKIQADPDSIQK
                     RFGIFGEPMMMGIILGIILGALAGYDVAGIFNVGISMGAVMLLMPRMVRILMEGLIPI
                     SESIRDFLQAKYGDRELYIGLDAAVATGHPAVISTALILVPITLLLAVILPGNKVLPF
                     GDLATIPFYMAFIVCFRKGNIVQSVITGTIVIAISLYMATNFADVHTQLLQQANMMPE
                     GTTMVTSIDTGGNLLKWGILKLSQLVSSFGIF"
     gene            26488..27540
                     /locus_tag="L3M60_000815"
     CDS             26488..27540
                     /locus_tag="L3M60_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBN6225509.1"
                     /translation="MKAAVLHGTNDMRFEDIEIKPCESDEVKIKVMAAGICGSDPPRV
                     LKHWKYPVPAIPGHEFSGVIAEVGKDVKNVKVGDRVVAIPFIPCNECEYCKRGLFSLC
                     DDHGMLGAKSFGAFAEYANIKATNVLPIGDMDFEDAAMIEPLAVAMHGVLNIGVQVGD
                     TVAVMGSGTMGQLVIQGLKIAGAGTIIAVDISDNKLRESKELGADIIINAKDVNPVEK
                     IKELTDGKGVDIALECAGSKITQEQCLLITKKKSKIGFLGIAYSDITLSEEAFENIFR
                     KELELKGFWNSYSAPFPGQEWTKGINLVNEGKIKLKEMVSHRFSLEDTYKAFEMIRDR
                     KEEFNKILILPQGVEK"
     gene            27540..28571
                     /locus_tag="L3M60_000816"
     CDS             27540..28571
                     /locus_tag="L3M60_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBN6225510.1"
                     /translation="MKAVVKTKPGYDNVEVLDVEEPKATGDKVKIKVEYSGICGSDIH
                     SFKGEYANIKAPVTLGHEFSGIVVEVGEDVKNIKVGDRVTSETTFATCEKCIYCETKD
                     YNLCQTRKGIGTQVNGSFAEYVLSREESIHVLPENVSLLSAALTEPLACCVHAALEKT
                     TIESSDTVLIIGPGPIGLLLAQVVKSQGATVIMSGVTKDKERLEIAKNLGVDRTVNVM
                     EESLADVVNEMTDGNGVNKGFDCSGFMPAVNEALRLLRKKGTFVQVGIFAKKLNEMDQ
                     EAIIQRELQYIGSRSQKPSSWITALDLMASGKVNTEALVTRTVGLDEFKEGITALMNG
                     EEIKVAVKS"
     gene            28662..29474
                     /locus_tag="L3M60_000817"
     CDS             28662..29474
                     /locus_tag="L3M60_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBN6225511.1"
                     /translation="MSTLRDHSNNIRKNIIKMVAEAKSGHPGGSLSIADMLTVLYFDK
                     MNVCAENPKMADRDRFVLSKGHAAPALYATLAEKGFFPEEELITLRKFGSKLQGHPDM
                     KKVAGIDMSTGSLGQGLSAANGMALAGKLDNKDYTVYTILGDGEIQEGQIWEAAMTAS
                     HYKLDNLIAFVDLNGLQIDGSNEEVMNVNPVDDKFKSFGWNVMVINGHSFEEIGNAVD
                     EAKKVTGKPTVIIAKTVKGKGVSFMENNAAWHGTAPNAEQAEQALKDLEGGL"
     gene            29477..30397
                     /locus_tag="L3M60_000818"
     CDS             29477..30397
                     /locus_tag="L3M60_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HBN6225512.1"
                     /translation="MGIATREAYGQVLKELAENKDIVVLDADLGKATKSISFKEVAPD
                     RFFDMGIAEGDMIGTAAGLATCGKIPFASTFAIFAAGRGYEQIRNSVAYPNLNVKIAA
                     THAGVTVGEDGGSHQAIEDISLMRGIPNMVVLNPADALEARQAILASIDYNGPVYIRL
                     GRAATPDVNSENYKFEIGKGTVLREGSDITVIATGIMVAKALEAAEELAKEGVNVEVV
                     NISTIKPLDEALIKETAKKTGKVVTAEEHSIIGGLGSAVCEALAETKDVVVRRIGVKD
                     VFGQSGTPADLLKHYGLTTEDIVKNIKELL"
     gene            30572..31018
                     /gene="rpiB"
                     /locus_tag="L3M60_000819"
     CDS             30572..31018
                     /gene="rpiB"
                     /locus_tag="L3M60_000819"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HBN6225513.1"
                     /translation="MKISIGCDHGGYELKEYIKENLINKGIEIEDVGTNSKESVDFPI
                     YAKKVSQNVQSKKSDLGILCCGTGIGMSIAANKFKGIRAAVVSDCFSAKATREHNNTN
                     VLCLGERVIGKGLAMRIVEEWLNSSYNGERHARRLQMIDDIEKENL"
     gene            31048..31713
                     /locus_tag="L3M60_000820"
     CDS             31048..31713
                     /locus_tag="L3M60_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBN6225514.1"
                     /translation="MSIICASIMCADQMKLKEELEALEEAGVKLLHCDVMDGIFVKNL
                     AMGPELLKSISENTTIPLDIHLATETPDKYIDMMSYIKPKYISFHVESSTNVRADIQK
                     LRNYGIGPVLAISPQTSVDKIEEYIGLVEGILVMTVNPGFAGQKFNLSVLDKLDKLTE
                     ILKDYDNPPFIEVDGNINKDTIKLMNGKKVDIYVVGTSALFNDKPPISYKDKIKELKE
                     SIK"
     gene            31823..33325
                     /locus_tag="L3M60_000821"
     CDS             31823..33325
                     /locus_tag="L3M60_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HBN6225515.1"
                     /translation="MGDLYKLQNGTDIRGIAYENDSKEVNLTVEEVRKIAKAFHIWLK
                     EKTKKDKVTVAIGTDSRITGSQFRSAVIETLTNDDCNVIDCEIATTPAMFMTTIMDGY
                     NSDGSIMITASHLPYYYNGLKFFTESGGLEKADIKEMLDIADKNDSVDYEKANKKGKV
                     ISKNLIEDYSNLLIDKIRKGVNSSKNYEKPFSGLKILVDAGNGAGGFFAEKVLHILGA
                     DTTGSQFLNPDGMFPNHIPNPENKEAMESICKAVLDNKSDLGIIFDTDVDRAAIVGKN
                     GKPINKNALIAVISSIVLEEHPKTAIVTDSITSEGLAKFINELQGRHHRYKRGYKNVI
                     NEAIRLNSEGEECHLAIETSGHAALKENYFLDDGAYLIAKILIKVAKLSLEGKTIEEL
                     IENLEEAREEKEVRIGINKEDFRPYAEDILKDLDSHVRSIDGWSVAPNNFEGIRVNCD
                     ESNGDGWFLLRISLHEPLLALNIESNKIGGAEQIYSKLKLFLEKYDLRGL"
     gene            complement(33637..35229)
                     /locus_tag="L3M60_000822"
     CDS             complement(33637..35229)
                     /locus_tag="L3M60_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6225516.1"
                     /translation="MVTKLKNLIINDSKAKFILLLLLFLLISILFEAVYDLNNNFFYN
                     INYYYKEFTIPYFYFFGTPLHLSKVELEFVCIGVILSSFLLIVLCFILYKKNKQTKVK
                     FLENVISLFSKVKIELTLILILILLILRFHAFTSNKMFYNTNLIIYNFIIISIVYIIY
                     ENIKYNNYNLKDMSITYVFFSNLLSVYNRKTINKKLAIVFLISIVMQAIIQNMVLIFF
                     MYIYYAEAIAIVILFTLVNTVFHLYIYNFLSAKFNYINNISKNVKNIENGDLKYKLEV
                     IGNDEISDLSKSINNITDGLENAVDSSIKNERMKTELITNVSHDLKTPLTSILSYVDM
                     LKNNDFDKETVNDYINILDKKSQRLKLLIDDIFEASKLSSGDVEFNITKTDIRELLIQ
                     SIVEFEDKIQNSNLDFIIETPEHAVFTMIDGKKTWRVFENLICNILKYSMPNTRVYID
                     MFIKEENIILTFKNISNDKLNLKPEELVERFRRGDVSRKTDGSGLGLSIAQNIIELEN
                     ADMEIIIDADLFKVMLTFRRVN"
     gene            complement(35198..35914)
                     /locus_tag="L3M60_000823"
     CDS             complement(35198..35914)
                     /locus_tag="L3M60_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225517.1"
                     /translation="MNQNILVVDDDREIVDAIEFYLKPDNFNVLKAYDGLEALDILIE
                     NDIKLIIMDVMMPNLDGLKTTLKIREKRNIPIILLSAKSEDMDKVIGLNMGADDYITK
                     PFNPLELTARVKSQLRRYINLGDFSNTNNSNDTNYILKSGGLELNTDTKIVSLDGEEI
                     KMTPLEYKILTLLMSRKGKIFSSREIYENVWNEDAYSCERTIAVHIRRIREKIEINPK
                     EPKYLKVVWGIGYKIEKLNY"
     gene            complement(36457..37257)
                     /locus_tag="L3M60_000824"
     CDS             complement(36457..37257)
                     /locus_tag="L3M60_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBN6225518.1"
                     /translation="MTFKNKKFTISKYRILSIILALCLILTAFSSYMNYSHKKFKDYV
                     VFYVQHQDDEVLWAGSAIVNAIKERGKNHVFVVLVSTGCGISVFDKYKKYENLTDIQK
                     SEYRNREFLASLNSLGVRRENIILLPETNTNGNTDFNYMEKIAINFEENFKNVTHIAH
                     TYKLDDHLQHLKNGSVIQNLYNAGKIKDAKYFVKPKFADKITFNNKIVYKAHSTEDYE
                     KVKNACQQYKIVDELEDREGIGYKSDHKSFDKLLRNKDVPAILHTPNL"
     gene            complement(37569..38300)
                     /locus_tag="L3M60_000825"
     CDS             complement(37569..38300)
                     /locus_tag="L3M60_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225519.1"
                     /translation="MNIFDILRDYLLVQVDKYNLNMDMISIVSKSLSSKEAIGNTKRK
                     DFPIIIGKEIMLEADFKGAKGQAFTSTPSTFEGSLKDILSLDLYDNPHDRSLFIASLN
                     AVMKYLGKTDRTIHCKNNEPEICAKNFPKFIKREFDNPKVAIIGYQPAIIDNIKDFFE
                     TRVLDLNPEFVDTIQYNVKIEDGIRDYEDVISWADLVICTVSTLCNNSIINFLSLNKP
                     VYYYGTTIAGASNILGLKRLCFCSK"
     gene            complement(38287..38982)
                     /locus_tag="L3M60_000826"
     CDS             complement(38287..38982)
                     /locus_tag="L3M60_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6225520.1"
                     /translation="MLKIRNLSLDLPNFKLQENSLNINNHEYFVLLGSSGSGKTLFLE
                     TLAGRYPSAKGSVSFKKNLISSLPPENRNIGFVYQNFELFPNMNVEDNISFPLKLRKI
                     PIDEQILKVNHLSKLMQIEDIKNRYPKNLSGGEKQRVAFARALIAEPDILLLDEPMSS
                     LDYITKKHISKILKDVYIKYKPTVIHVTHDISEAMFFAHKIGIMNNGKINHIFDLNEE
                     IKHKGESFFYEYI"
     gene            complement(38984..39775)
                     /locus_tag="L3M60_000827"
     CDS             complement(38984..39775)
                     /locus_tag="L3M60_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225521.1"
                     /translation="MKNKFFTILLTGFFAVVFLFIVSPLLMLIIKGISYVPICLKSVE
                     VQYAITLSIKTSLISTIVCLLLAIPVAYFLHITKLPFKKLIIQIINLPMSLPHLVSGI
                     ALLLLFGRMGIGDSIYKILKLDFVFTKQGIILAQVFVNLPLTIKILHTSLNESNEKMI
                     FVARTLGCNSWEAFKFIILPNLKTGIISATVMTWSRALGEFGAVAMIAGSTRMKTEII
                     PTSIYLNMSTGDIDIAIGIAVILIFISLTCLMLFEIFFNREVDKN"
     gene            complement(39785..40597)
                     /gene="modA"
                     /locus_tag="L3M60_000828"
     CDS             complement(39785..40597)
                     /gene="modA"
                     /locus_tag="L3M60_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6225522.1"
                     /translation="MFKKFSVLILSMIISLSITACNSKENKANNESNAKVTTANDSKE
                     KETLFVYSGAGLKKPMDEIAQKFEKENNIKIEYSYAGSAQLIAQIETSHKGDAFIVGS
                     EPIYSKAFEKKLVGEHKTVAHHTPAIVVPKGNPANIQKLEDLNRDGVKLIFGDKESNA
                     IGKTTQKILEKNNLKSINDNVVSTAATVNEMIVQLTSSKADATIATKDSVFGNEDVEV
                     IEIAQDKNIDQLISAGIVNYSDKKDLANKFINYIASDEAKQIFKKYGFEPVK"
BASE COUNT        15958 a         4700 c         7154 g        13022 t
ORIGIN      
        1 tagagtctta gggagaaagg agaaatatat tttttaataa tatttgttaa tttaataaca
       61 aaaaatgaaa gggagtgatt tggtgaagtt tgaattgatg gattttttaa tgaatccttt
      121 tgtccttatg tttgctgcag taattacagg aattttattt ggaaaaatta aatttgggaa
      181 gtttaatttt ggggtatcag gagcattatt tacaggatta tttattggat ggcttgcata
      241 tagcttggga aatttaataa tagcaaaagg agaaacagca gcaggatata aggcagcaac
      301 tgtaatgatg gggaatggaa ttatttctcc tgacttcttt gatttctttt taataatatt
      361 tgttgcagca gttggtctat tggcagcaaa agatatgaaa gctgtattaa agaagtatgg
      421 agcaagattt gtaattttag gcgttcttat aactttcata ggtggctcta tgacttatgc
      481 aatgacttta ttatcaagtg ataaaggaag tagcgcttat gaagtttctg gtgtttacac
      541 aggagcatta acaagttcac ctggattagc cgctgcttta gaaacagctg gtaaacatgc
      601 agaagatgta tcaaaagaat ttgaaaaagc ttcaacaaaa gataaagaag aaattttaaa
      661 agtagtcgac ccagaaggaa aattagatgt gagcacaact acatcactga ctcaagagca
      721 aatagataag tacattgcat atgctgaagc tggtgttggt ataggtcatg cagtcgcata
      781 tccttttgga gttttaatag taatcttagg tgtaaatttc cttccaaaat tatttaggat
      841 ggaccttaaa gaagaaagaa gaaaatatga gaaggaaatg aaagaagcaa gagatagtgt
      901 ttctggtaaa acgataccag aagtcccatt taatataata acatttttct tgacatgttt
      961 agctggatac ttagttggag gaatacatgt atttatggga ccactagggt actttacact
     1021 aggagcaaca ggaggttcac taattgtatc tctagttctt ggatatatag gtaagatagg
     1081 ggtaataaat tttagaatgg aagaaaaggt tttaaatatt ttaaaacaaa tagggctagt
     1141 atttttctta gcaatagttg gattaagata tggtggaaaa gtagttgatt caattatgac
     1201 atctggtatg cacctagctt tagtagcaat agcagtgggt gttacagcta tgatgatagg
     1261 attcttagtt ggtaaatatg tatttaaatt aaattggatt ttactatctg gagctgtctg
     1321 tggtggtatg acatcaacac caggccttgg agctgctgtt gatgctcttg atagtgatga
     1381 cccagcagca ggttatggtg ctacgtatcc atttgcatta ttaactaaag ttatattggt
     1441 tatagtttta cacaaacttc caatgtaaat aagattttat atggggggat atttatgaat
     1501 aatgaaggaa catttgaaga actaagtgga agattaagaa ataaaaaatt gtgtgattta
     1561 ataatgaatg cagatgaagc tgctaaattt ataaaggatg ggatggttgt tggagttagt
     1621 ggatttacac cttcaggtta tccaaaagca gtaccattag cagtttcaga aagagctaaa
     1681 agtggagaaa agataaaatt aactgtatat tcaggagcat ctttaggtcc agaagttgat
     1741 ggagcatggt cagaagcagg tataattgaa agaagacttc cttatcaaac taattcaata
     1801 cttagaaata atattaataa aggtgttgtt gattatatag atatgcacct aagtcattct
     1861 actcaatttt taaattatgg aactataccc aaagtagatg tagctatagt agaagcactt
     1921 gcaataactg aagatgggaa tattatacca actagtggaa taggtaattc tccatctttt
     1981 ataaaaagtg cagataaagt aatagtagag ataaatcttg caaaaccaat ggaaatggaa
     2041 ggtatggcag atatatatat tacggaaaat ccaccaaata gaaaaccaat agaaataaat
     2101 catcctaaag atagaattgg aacaacatat ataccttgtg gtttggataa aattgcagga
     2161 atagtaatta ctaatatgca agataaaacc cgaccattgg gtgtagtaga tgaagcttcg
     2221 aaaaaaatat caaataatat tattgctttt ttaagagaag aagtaaaggc aggtagactg
     2281 tcaaagaatt tacttcctct tcaatctggt gtaggaagtg tagcaaatgc agtattatat
     2341 ggattatgtg aatctgaatt tgaaaattta acttgttaca cagaggttgt acaggattct
     2401 atgttggatt taataagaat gggcaaagtc acaatggcat ctactacatc tgtaagtcca
     2461 tctccggaag gattaataaa atttgaaaaa gatattgatt tttttaagga taagattata
     2521 ttaagacctc aagaaattag taataatcca gagatagcta gaagaattgg tgtaattgca
     2581 atgaacactg caattgaagt tgatatttat ggaaatgtaa attccactca tataatgggg
     2641 tcaaagatga tgaatggaat tggtggttct ggtgattttg ctagaaatgg agcaataact
     2701 atatttagta ctgagtcaat tgcaaaaaat ggtgatatat catcaatagt acctatggta
     2761 tctcatgtag accacactga acatgatgta atggtaattg taacagaaca aggatatgct
     2821 gatttaagag gtcttgcacc tagagaaaga gctataaaaa taatagaaaa ttgtgcccat
     2881 ccagattata aagaacaact tagagattat ttaaatagag cttgtaaaag tggagcaaaa
     2941 caaacacctc acatattgga tgaagcttta tcatggcata gtaaatttat gagtacaggt
     3001 acaatgaaaa aggctgaaac ttttaaaagt gcattataaa atatttaata aattggaggt
     3061 agattttaaa tgggaaaagc agtggaaaat tttgaagatt taagtaagga atacattaat
     3121 ggttatattg agagagccag aaaagctcaa agagagtttg aatgttatac tcaagaacaa
     3181 gtagataaaa tagtaaaaat agttgggaaa gtggtgtatt ataatgctga atatttagct
     3241 aaacttgctg tagaagagac tggaatggga gtatatgaag ataaggtagc taaaaataaa
     3301 agcaaagcaa aagttatata taacaattta aaagataaaa aatcagttgg tataattgat
     3361 atagatagag aaactggaat aacaaagata gctaaaccgg ttggagtagt agcggcaata
     3421 actccttgta caaatccaat cgttacacct atgagcaatg caatgtttgc gcttaaggga
     3481 agaaatgcaa ttattatcac acctcatcat aaagcaattg gatgtagtac aaaaactgtt
     3541 gagatgatta acgaagaatt agaaaaaata ggagcaccag aaaatttaat acaaatactg
     3601 gaccaacaat caagagaaaa cacaagaaat ttaatttcat cagcagatgt agtaattgcc
     3661 actggcggaa tgggaatggt aaaagctgca tactcaagtg gaaaaccagc acttggtgtg
     3721 ggagcaggaa atgttcaatg tattatagat agagatgtag atattaaaga agcagtacct
     3781 aaaatcatag caggaagaat ttttgataat gggatcattt gttcaggcga gcaatcagta
     3841 atagtagcag aagaaatgtt tgataagata atggatgaat ttaaaaataa caaaggtttt
     3901 atagttagag ataaagcaca aaaagaagct ttcagaaatg ctatgtttgt aaataaatct
     3961 atgaataaag atgcagtagg tcaatcagtt catacaatcg caaaaattgc aggtgttgaa
     4021 ataccagagg atacaaaaat aatagtaata gaagctgatg gaccaggaga agaggatatt
     4081 attgcaaaag aaaaaatgtg tccagttata tctgcatata aatataaaag ttttgaagaa
     4141 ggtgtagcta ttgcaaaagc aaatttaaat gtagaaggaa aagggcatag tgtatctata
     4201 cattcaaata cagttaagaa tatagaatat gcaggagaaa atatagaagt atcaagattt
     4261 gttattaatc aatgttgtgc tactagtgct ggaggaagtt tctttaatgg tcttgcacct
     4321 actaatactc taggttgtgg ttcttgggga aataacagta tctctgaaaa tcttgattat
     4381 aagcatctaa ttaatatttc tagaattgct tattatatgc cagaaaatga agttcctaca
     4441 gatgaagaac tttggggata aaatttagac aaaaaatatt cattctaaat taaaataaga
     4501 atatatttta atttagaatg aatttaagac ataaattaaa tttaaattag tctattagac
     4561 caaatttaaa atattaagag acatatagaa gaaggaagta ttataattta aaataaataa
     4621 aaaattctat aaaagagaag gagtggtttt attatggcat taatgacagg agctcaatat
     4681 attgaaagtt tgagaaaatt aaatactaag gtttatatgt ttggtgaaga ggttaagaat
     4741 tgggttgacc atcctatgat tagaccatct ataaattgtg tagcagcaac ttatgactta
     4801 gctcatgacc ctgaatatgc agatttaatg acagttactt caaatatcac aggtgaaaaa
     4861 ataaatagat ttggtcatct tcatcaaagt actgatgatt tgataaaaaa agtaaaaatg
     4921 caaagactat gtggtcaaaa aacagcttct tgtttccaaa gatgtgttgg tatggatgca
     4981 tttaatgcag tatattcaac aacatttgaa tgtgataaag cccatggaac taattatcat
     5041 gataattttg taaaatactt aacttatata caagaaaacg atttagtagt tgatggagct
     5101 atgactgacc ctaaaggaga tagaagttta tcacctagtg ctcaacctga cccagatatg
     5161 tttcttcata tagtagaaag aagagaagat gggataattg taagaggtgc aaaggcacat
     5221 caaactggtt caataaattc acatgagcac cttataatgc ctactataag catgactgaa
     5281 gcagataaag actacgctgt atcatttgca gttccttcag atgctgaagg agtatttatg
     5341 atttatggaa gacaatcttg tgacactaga aaattagaag aaggtgcgga tgttgattta
     5401 ggaaataagg agtttggagg tcaggaagct ttagtagtgt ttgataatgt atttgttcca
     5461 aatgatagaa ttttcttatg tggagaatgg gatttctctg gtatgttagt agagagattt
     5521 gcaggatatc atagacaaag ttatggtgga tgtaaagtag gagtaggtga tgtaataata
     5581 ggagcagcag ccttagcagc tgattataat ggagcaaaca aagcttcaca tataaaagat
     5641 aaactaatag aaatgactca ccttaatgaa agcttatatt gctgtggtat agcatgttca
     5701 tcagaaggtc acaagacaga agctggaaac tatcaaatag atttacttct tgcaaatgta
     5761 tgtaaacaaa atgtaactag atttccttat gaaatagtaa gacttgcaga agatatagct
     5821 ggagggctta tggttactat gccttcagaa aaagacttta aatcagacct taaagttgga
     5881 acaagtggaa tgactattgg cgaagtatgt aacaagtatt ttaaagcttc ttcagtagct
     5941 tcaacagaag aaagaatgag aatattaaga tttttagaaa atatatgtct tggttcatct
     6001 gctgttggtt atagaactga gtcaatgcac ggggcaggtt caccacaagc tcaaagaata
     6061 atgatttcaa gacaaggaaa cataaatcaa aagaaagaat tagctaagac aattgcagga
     6121 ataaaaaaag aagaagcttt aaatttataa aaagttgata tataaaggga aattatatat
     6181 taatgtttat taattaatga aaaaatatgg agtataagag ataattataa aattatggtg
     6241 gtttttagct aagtaaagaa attttttata aggagaattt acacatgacc tgtggagtta
     6301 gattttgtgg cggctgtaac cctagatacg aaagagggaa ggctcttgca ataatcaaag
     6361 agcacttcaa tggtagagta gattttagta tagcacaaga gaacttggag tatgatttgc
     6421 tacttataat tggaggatgc gcaactctat gcgcatccta tcaacaatat aaagtgaaaa
     6481 atgaatgtgt taagatgtgg gattttagcc atatagaaag agttataaat gtgattgaat
     6541 cagcaatttg tgaaacttaa aaaagctaaa tggggggatt tcattgagtt ggcgagaatt
     6601 gtatcaaagt aagttatgta gtgctacaga agctgtaaaa cagattaaaa acggagatac
     6661 tgtagtattt gcacattgtg taggtgaacc tcctgcacta gtagaggcta tgattgaaaa
     6721 tgctgaacaa tataaagatg ttgagattaa acatatggta agcttgggta gtggtggata
     6781 tactgctaag ggaatggaag ctcattttag agtaaatcca atgtttgtaa gtggaaatgt
     6841 aagaaaggca attgaaaacg gagatggtga ttttacgcct acattcttcc atgaagtacc
     6901 aaagttgctt agagaaaaaa gattaaaatg tgatgttgtt ttagcacagg taactccacc
     6961 tgatgaacac ggatattgta gcttagggac atcagttgat tatacttatg aagctattaa
     7021 aactgctaga acagtaattg ttcaagtgaa tgaccagttt cctagaactt atggtgaggt
     7081 agtccatgta tcagattttg actatatagt ggaaaaatca caacctttat atgaattaga
     7141 acctgcaaag attggagaag tagaagaagc aattggtaaa aactgtgctt cattaattga
     7201 agatggttct actttgcaac tagggattgg tgggatacca gatgcagtta tgttattttt
     7261 aacagataaa aaggatttag gaatccattc agaaatgatt tcagatggga ctttggctct
     7321 ttatgaaaaa ggtgttataa atggtaaata taaaaatttt gataaagaga aaatgacagt
     7381 tacgttctta atgggtacta aaaaattata tgactttgta aataataacc ctgcggtaga
     7441 ggtaaaacct gtagactatg taaatcatcc agcaattata atgaaacaac ataagatggt
     7501 ttccataaat tctgctatac aagttgattt gatgggacag gtagttgcag aggctatggg
     7561 acttagacaa tttagtggtg ttggaggtca agttgacttt atacgtggag tgtctatggg
     7621 agaagatgga aaagcaatta tagctatgcc ttcaataact acaaaaaaag atggtacagt
     7681 aatttcaaaa attgtatcta ttgtggatga aggtgcacct ataacaacat caagaaatga
     7741 tgttgactat attgtaacag aatatggtat agcagaatta aaaggaaaat ctcttagaga
     7801 aagagctaga aatttaataa atatagctca tccaagtgta agagaaagtc tagcaataga
     7861 gtttgagaag agatttaagg aaaaatatta ataatagaga ggtgttaata atggaagcac
     7921 ttagagtagt tccaaagata ttttattttg atacatttaa agaatttaat gaagaattta
     7981 aaataggaaa aaatgattta gtaattacta atgagtttat atatgagcca tatatgaaac
     8041 ctcttggaat agatacaaat ttaatttttc aagaaaagtt tggaacaggt gaaccatctg
     8101 atgaaatgat agattccatg acaaaggaaa tgaagaaata caattttgat agaattattg
     8161 cgtttggagg aggaacaata gttgatatat gtaagatatt agcgcttgat gttccagaaa
     8221 agtctattga tttatttgaa ggagaagtac cacctaaaaa aataaaagag ttagtagtag
     8281 ttcctacaac atgtggtaca ggtagtgaag taacaaatgt agctattgca gaattaaaat
     8341 ctaaacatac taaaaagggg cttgcagtag aagaaacata tgcagactat gcagtgctaa
     8401 ttccagagac agtaaaaggt ttaccttata aattctttgt tacaagctct gtggatgctc
     8461 ttatacatgc tattgaatct tatctttctc caaaagcaag tccatttaca gaaatgtatt
     8521 ctctgcaagc aataaaaatg attatggatg ggtataaaaa gatagttgat aaaggagaag
     8581 aagagagatt taatcatctt agagactttg tccttgcatc aaattatgca ggaattgctt
     8641 ttggaaatgc tggttgtgca gcagttcatg cactatctta ttcaataggt ggagctttcc
     8701 atgttgcaca tggtgaagca aattatcagt tctttactga agtatttaag atgtattcta
     8761 gaaaaaaacc taatggaaaa attataaaat gtacaaaaat tttagcagat gcccttgaat
     8821 gtgaccctaa ttgtgatgtt tatggagaat tagaaaagtt tttaaacaag ttaatagcta
     8881 aaaaagcttt gagagaatat ggaatggtgg aagctcagat agatgaattt acagatagta
     8941 caatagcaaa tcaacaacga ttacttgcta ataattatgt agaacttaat agagaggaaa
     9001 taagagagat atttgcaaat ctttattaat aaaatttttt atttatatgt atagtcaaca
     9061 taagcaatgt taattatatt tatgtacaaa taaaaacgag ggaacttaaa tttaattaaa
     9121 gttaagttct cttattttta tatggagtta aatgtaagta tatttttgat ttaaaatgta
     9181 cttacattta agtctattac tttatttgga acaatttgta gaatttcaaa ttccttgaca
     9241 gtaataagta agtaattgta taatcaaact gcaatatatt aataatatta gtgaaaatga
     9301 aaggatataa aatatgtata aatcagaaaa tttttcaaaa aaatctgtat taataaattc
     9361 atttttaagt aaatttttaa aaataattgg atggggttta gtaagtttat ttacattatc
     9421 agcattagta ctttcaacta tttccattat aatcggatgg gctgtaatac ttcctatcca
     9481 agtatgtgca gtatttttaa ttataggagg agtttgtatt tttaaaggta agaggttagg
     9541 agaccaaatt tctcgttata aaaaatattg tacaattata aacaatagaa atattattcc
     9601 agtagaactt attgcagaaa atacatctaa atcattaaat tttataataa aagatgtaca
     9661 aaagatgata gacaaaaatt actttataaa tacttacatt gataaaagaa ataatcagat
     9721 tgtactgaca aatgaagatt ttgttccaac aagatatgaa gatgtagcat atgaagttaa
     9781 agaagaagaa aagactgatg aagacaatga agttgataca attataaata aaggaatgaa
     9841 ttatctaaca catataaaag aagctaataa aaatattaaa tcagaatcaa tgtgcgataa
     9901 aattgtacaa gtagaagatg ttacaagtaa aatatttgat gttgtaaaac atgacccatc
     9961 aaaacttact caaatacaaa aatttatgga ttattatctt ccaactactt tgaagctatt
    10021 aaattcatat catacattgg aagaacaagg cattgataga gaaaatataa caacaactat
    10081 ggaaagtatt gaaaatacaa tgaatacgat tgtagtagct tttgaaaatc aattggacta
    10141 cctgtttgaa gatgaagcaa ttgatatttc tacagatatt actgttttag agaatatgtt
    10201 agttcaagaa ggattaacga gtggtaaatt ttaacttcaa tagaaaaaat attctgttat
    10261 ttttaaacaa ttaattagtc ataaagtata aaatatgtgt tagataagtt aagcagtact
    10321 ttatgaagga agaggagaga aagatataat gaataattta gatgatatac cagttatgcc
    10381 aactttgacg cttgacccat ttggagaaga atcaaacact agtgatatag ataattctga
    10441 cttattaatg aaaaaggatg aaaaagaccc agaagaagaa aagctgtctg aatctgaacg
    10501 taagatggta aaggaatttg cggaaaaaat agatattaca aatacaaata tgattttaca
    10561 atatggagct ggtgctcaaa aaaaggtggc gagcttttct gaaacagctt taaagagtgt
    10621 caaaacaaga gatttaggag aagttggaga tatgcttaca aatctagtta ctgatttaaa
    10681 agcattttca gcagatgaaa aagaacaaag tggttttttg ggaattttta aaaaggcaaa
    10741 taataaaatt tcaaatctaa aaactaagta tgatagtgca gaggtaaatg tagataaagt
    10801 aagcaaagaa cttcaaaagc atcaggttaa aatattgaaa gatatagcaa tgcttgataa
    10861 gatgtatgag ttgaacttag cttattcaaa agaacttact atgtatatta tagccggaaa
    10921 acaaaaacta aaagatatga aagaatatga gatgccaaag ttgagagaaa aagctcgttt
    10981 atctggttca acagaagatg ctcaatctgt aaatgatatg gtttcacttt gtgacagatt
    11041 tgaaaagaag attcatgact tagaattaac tagaatggta tcgcttcaaa tggcgccgca
    11101 gataagattg gttcaaaata gtaataatct gatggcagaa aagattcaat caactatagt
    11161 aaatactatt ccattatgga aaaatcaaat agtacttgct cttggaattt ctcatttaaa
    11221 tcaagctatg aaagcacagc gtgaagttag caatatgaca aatgagcttt tattaaagaa
    11281 tgcagaaact ttgaagatgg gaacaataga aacagcaaaa gaatcagaaa gaggaattgt
    11341 agatatagat acaattaaga aaactaatca atctttaata tctactattg atgaggtcgt
    11401 taagatacaa catgatggta gaataaagag acaagaagca gaagttgaat tatcaaaaat
    11461 agaaaatgaa cttaaaagta agcttcttga atttacagtt aaataaaaat tatactaaga
    11521 taaaaaagag actatcccaa aatagagata agtctctttt tcattgttta aatgattaaa
    11581 gtatatactt atattataaa tttctattat cttttaacat aacatcatga gccccacctt
    11641 caactatact agtagaagat acaagcgtaa gtttagcatt tttttgaagt tcaggaatac
    11701 taagtgctcc acagttacac atagtagaac gaactttact taaagaaaga gttacgttgt
    11761 cttttaaaga accagcataa ggcacataag aatcaacacc ttcttcaaaa gaaagtttct
    11821 tatctccacc aaggtcatat ctttgccagt ttctagcacg agcagaacct tctccccaat
    11881 attctttcat gtaactacca tttatattaa ctttgttagt tggactctca tcaaagcgag
    11941 agaagtatct accaagcatt ataaaatctg cacccatagc taaagctaga gttatatgat
    12001 ggtcataaac gataccacca tcagaacaaa ttggaatata aatacctgtt ttttcaaagt
    12061 attcatctct agcttgagca acttcgatta tagaagtagc ttgtccacgt cctatacctt
    12121 tttgttcacg agttatacat atagaaccac caccaatacc aactttaacg aagtcagcac
    12181 cagcttcagc taggtaaagg aatccttcac ggtcaactac atttccagct cccactttaa
    12241 ctgtatcacc atatttttca cgaatgaaat caagagttat tttttgccac tcagaaaaac
    12301 cttctgaaga atctatacat aaaacatcag caccagcttc aactaaagca ggaacacgtt
    12361 cagcaaagtc tctagtatta ataccagctc caactacata tcttttagaa gaatctaaaa
    12421 gttcaagtgg attttcttta tgagaagaat aatctttacg gaataccatg tacataagat
    12481 taccattatc atcaagtaca ggtagagagt taagtttgtg gtcccatata atgttgtttg
    12541 cttcttttaa agtaacatct ttgttagcgt aaactataga tgacatagga gtcataaatt
    12601 cactaacttt agtatctaaa tccatacgac ttatacgata atcgcgactt gcaacaatac
    12661 ctaataattt accagttgaa gttccatctt cagtaacagc tactgtagaa tgtccagttt
    12721 tttcttttaa gtcaagtatg tctttcaatg tgttatcagg tttaatattt gaatcactta
    12781 caacaaaacc agctttgtgg gatttaacac gtgccaccat agcagcttca ttttcaatag
    12841 tttgagaacc gtatataaat gaaataccac cttctttagc aagtgcaata gccattttgt
    12901 catcagaaac agattgcata atagcagaag tcaaaggtat attcatataa atatctgctt
    12961 cctcgccctt tttgaattta gtaacaggtg tttttaaact tacattagca ggtatacaat
    13021 cagtagaaga gaaacctggt acaagaaggt attcactgaa agtatgtgat ggtgtatcaa
    13081 aataaaacgc cattttttaa tcactcctta tatttttata ctttttataa ttgatagtat
    13141 taattataac ttattcttta attttattaa agtattttgt caaatattga gatttttgaa
    13201 aaatttaaac tattttaatt gaatttgttt aatatatatt gccttatagg gtaaagttgt
    13261 atattaaata atgtttagta tttaatagta gctaatttga aattaatatt tagttattca
    13321 tacattaaag ttgtatattt tatgttgata atgtgaacct agagtgtata aatgtcaaaa
    13381 gtatagataa attaaatcta tattagtata tccaaaagta cccttaataa atttgtttaa
    13441 ttgaattatt atcgtttttt ctattaaatt taaatatttt ataaaaatag tattatttat
    13501 aaaactagac accctaaaat actacataaa taaaattaaa caaaaactaa aatggcaaat
    13561 gtcattatct aaagtgacat agaaacggtt tcttaaagtt gttactataa aatataaact
    13621 ttataataaa agtataacaa aagggaatca tatcgatata gaatataatt caaaaggagg
    13681 caaaattatg agtagttcta atcaatcgac aataaagaca tctatacagc gagtagggaa
    13741 atttttaagt ggcatggtaa tgccaaatat aggagctttt atagcatggg ggcttataac
    13801 agcattattt ataccaacag gatggatgcc taatgaaaaa ttgagtacta taggtgaccc
    13861 aatgataaaa taccttttac cactattgat agcttatact ggtggtaaag caatagcagg
    13921 acaaagaggt ggtgtaatag gtgcagcagc agctatggga gtaattgttg gggcagatat
    13981 accaatgttt atcggagcaa tgataatggg accatttgca ggatggataa ttaagaagtt
    14041 tgataaattt gtagatggaa aaataccaac aggatttgaa atgctagtaa acaacttttc
    14101 aataggtata ataggtatgc ttcttgcaat actaggattt tatgcaattg gaccagcaat
    14161 agtagcaggt acagctctta tagaatctgg agttcaattt atagttagca aaagtttatt
    14221 accgttagtt tcagtattta tagaacctgg aaaagtactg ttcttaaaca atgcaataaa
    14281 tcatggtata ttaggaccta taggtatagc tgaggcaaaa gaagcaggta aatctataat
    14341 gttcttatta gaaagtaacc ctggaccagg tttaggagtt ctattagcat attggatgtt
    14401 tagtagagga agtgtaaaac aatcagcacc aggagctgta ataatacatt tctttggtgg
    14461 tatccatgaa atttatttcc catatatttt aatgaatcca gttttaatat tggctacaat
    14521 agcaggtgga gcagcgggaa tactaacttt ctcaatatta ggagctggac ttgtagcagc
    14581 accatcacca ggaagtatat ttgcacttat ggcactagct ccaaagggag gattacttcc
    14641 agtattagca ggagttgcag tggcaacagt tgtatcattc ttagtggcag caccatttgt
    14701 taagagagct tcagcaaatc aaagtgaaga agacaatact tctttagaag aagctaaagc
    14761 aaaagtgagt gatatgaaat ctgtaagtaa gaactctaag aaagatattg aagaaaaaca
    14821 gctggaagta aatgaaatta agaagattgt atttgcatgt gatgcaggaa tggggtctag
    14881 tgcaatggga gcttctagat ttaaaaatag aataaaaaat ttagatttaa atatagaaat
    14941 tacaaattca tctgttgata atcttccaga tgatacacaa atagtagtaa ctcataatac
    15001 attagttgaa agagttgcta agaacaattc aagtgttgag atagtttcaa taaacaattt
    15061 tttaaatgac ccaaatttag atgtactatt taaaagacta gaatctaaat aaaataaaaa
    15121 gtaccactaa ttaggagtgg ataatttgaa aaaaaagaag attacatcta ggcaaaaaaa
    15181 gataatacta atgatagtag aaaattcaaa gaaaaatatt ccaataacta tttctgaaat
    15241 tgcagggatg ttggagttaa gttcaagaac tgtacttaga gatatgtctg gaattgaaaa
    15301 atggtttgat gaaaatgatt ttaactttgt aaaaaaacca ggagtaggat tgattttaga
    15361 ggaaagtata gaaaatcaaa attttataat tgaattatta gaagaagaaa aaatagaaaa
    15421 agaatacagc aaggaagaaa gaaatttaat catattatca aagctactag tttctaacga
    15481 gccagtaaag tcttattatt ttgcaaaaat attaaaggta tctgaagggg tattgaataa
    15541 tgattttgct ttagcaagta aatggctgga aagatttgat attgagcttg ttagaaaacc
    15601 agggctgggt gtttacttaa aaggtcaaga aaaaagcttt agagaagcat atgtaaatct
    15661 tatatatgat tcatttaatg aaaaagaaat actagatatg gtcagaaata taagtgaaaa
    15721 tatacaaaca gataaaacaa tagaaatttt aagtgaaaat agacttttaa atttaatgga
    15781 taagtgcata ataagaaaag ttgaaggtac attaacaaag aaactttcag atttagatgt
    15841 aaatttagct gatagtgcat atataggtct tgtagttcat atttcattgg ctttacagag
    15901 gataaaaaat ggtgaaaata taactatgga taaagaattt ctaaaagaat tatctataac
    15961 agaagagttt aaattagcta aagaaatcgt aaaaggtatg gaaatagatt ttagcatgga
    16021 tataccacta gatgaggttg gatatataac tatgcatata agaggtgcaa agcaaaggtc
    16081 atcttcaaat cacaatgctt taaacttaga tgatatagaa gtaatggata ttacaaataa
    16141 gatgatagat ttagctgaag atgaatttaa aataagttta aaaaatgacg agaggttatt
    16201 taaagaccta gcaaatcact taggaccttc tataaataga ttgaatatgg gattagaaat
    16261 aagaaatcct cttttagatg aaataaaaag caagtattca tatgtctata atggagttga
    16321 aaagatttct agaattataa aagataaact aagtataaat tcaatacctg aatcagaaat
    16381 aggatatatt gcaatgcact tcgcatcagc catagaaaaa aatcttatga tgtatgctaa
    16441 tataaatatt gttgtggcat gtcccactgg aataggtact tcaagattcc tatcaactaa
    16501 gattgagaat aaatttccta atttaaatat acttgaaacc atctcagcta taaacataga
    16561 tgaagagtat ttaaaggaaa aagatgttga tttaattgtg tctacagttg agttaaatac
    16621 aagtttaaat tatatatgtg taggtccatt tatgagttta gatgacgaac agttaatcaa
    16681 ggaaaaaata aaatctatag ctcaaaataa attaatcaat ttaaatgtaa aaaatggtac
    16741 taaaaataag aataaaatat atgcacagat aacagaaagt atgaatatag gaaaagatat
    16801 tcttcaattt ttagaggaaa ttaggtttga aaaatttgaa agtaaagatt taagtgagct
    16861 tattgaagac tcatcaaaaa tatttgcaaa atctagtgaa gatataatat ctataaaaga
    16921 atctttaaaa gaaagattaa aaatctcaat tccatatata gaagaatcaa aaattctatt
    16981 attacattgt attagtgaaa gaatagatat tatgaagtta tcaataataa agctagagaa
    17041 tagaatcata ctagattcaa atgaagaaat cgacaatgta gtttttatgt tacttcctaa
    17101 aaattcacca agttatcaaa gacaaataat gagtgaaata agtggttcat taatagataa
    17161 tataattttt acgaataaga taaataaatt ctctatagaa gagatgatat tagaggtaaa
    17221 agatatagtt tttaattttt atacgaacag attaaaagca tttatagata aataaaatta
    17281 atattgaaaa ggaaggttga tactatgaat aaagtattga atgagaataa tatattttta
    17341 gggttagata gtgtttcaaa ggaagaagct ataactttag caggaagaaa gttagttgaa
    17401 aatggatatg taaaagaaga atatatacca gcaatgctag aaagagaaaa agtaatgact
    17461 acatatatgg gaatgggagt tgctattcca catggtgtaa acgaagcaaa aaaagaaata
    17521 ttatcttctg gaatagttat attacaattc cctaatggta tagattttga tggtgaaaaa
    17581 gcatatttat taataggaat agctggtgtt ggagatgaac atttagaaat actatctaat
    17641 atagcagtag ttttagatga tgacttaaca gaaagactaa aaaattcaaa tgacaaacaa
    17701 gcttttatgg aagcttttgc caactaatct taggaggact ttaaaatgaa aaaggcaatt
    17761 cagtttggag caggaaatat tggaagaggt tttattggag gcttattagt taagtcgggt
    17821 tatcatgtag tatttgctga tgtaaatgag gagatattaa actcaataaa caaagataaa
    17881 aaatatacta tacacataag agatgtagaa tgtatagatg aagtaattga taatataagt
    17941 gcggtttcat ctataaaaga agaaataata gatgagattg tagaggctga aataataact
    18001 acagcagttg gacctttagt tttgactaaa atagcttcta caattgcaaa aggcataaag
    18061 aatagaaaag aaaagggatt aacaagtaat ctaaatataa tagcttgtga aaatgcaata
    18121 tacgcaagct cttctttaaa agaagaggta cttaagtatt taaataagga agaagtggaa
    18181 tatttagaga tgtacattgg ttttccaaat tgttcagttg atagaatagt tcctcctggt
    18241 aaaaatgaaa atcctcttga tgtaacagtt gaaaattttt atgaatggaa tgttgaaaag
    18301 caaggattta aaggagaaat accaactata gttggtatga atttagctga taatcttatg
    18361 gcttatatag aacgtaagtt gtttacacta aatacaggtc atgctataac agcgtatata
    18421 ggatatttaa aaggatataa gactatagaa gaaagtataa aagataaatt tatctatgac
    18481 atagtaaaaa gtgcaatggt agaaagcgga gaaggattaa ttaaaaaata caattttgac
    18541 tcagaagcac actataaata tattgataaa attttaaata gatttaaaaa tccatatctt
    18601 aatgatgatg tattaagagt tggaagggag ccattaagaa aattaagtga taaagataga
    18661 ttgattaaac ctttaatgac agcaaaatca tatgacctat cagttgacaa tttgattctt
    18721 ggaataggtg cagcgcttca ttataataat agtgaagatg ctcaaagtgt agagttacaa
    18781 gagttgataa agtctaatgg ggtaaaaaaa gctgttgcta aaatagcgaa tataaataat
    18841 gatgaagaac ttttaaataa tatcgaaaaa agttatatat ttatggaaaa tttataaatt
    18901 tgcttgaaaa aagttagttc tgataaagta agcataacat attattctac acagtttgaa
    18961 ttatttgttg aataatagag aattataata tataattaaa aagaataaat taatatattt
    19021 acctgtatat gtttgataat atggttcaaa tgtttctact aagctaccat taatagcttg
    19081 actacaggag tgaagaagat atagtttata cttattttaa attatatatt aagttaatta
    19141 ctttgaattt taagctcctg tctatgatat agacaggagt ttttttatta tcttaatata
    19201 aaatacttac aagaatagat agcaaagtta gagcaaatat aattttttta ttaatataac
    19261 tttagaaagg attgggtgga agtttaatga aggctttaca agaaaagata ttgagagaag
    19321 gcagtgtttc aggaaatgat atactaaaag ttgatagctt tttaaatcac caaattgatg
    19381 tagctttttt aaatgaaata ggaagagagt ttaaagagag atttgaagga gaaaaagttg
    19441 ataagatatt tacaatagag gcttcaggaa tagctatagc atcaattgtt tcacagtatt
    19501 ttgataatgc acctgtagta tttgcaaaaa aatcggaatc taaaaatttg gataaagatg
    19561 tatatgaaac taatgtatat tcatttacta aggcaagaga atattctgtt aaagtttcta
    19621 aaaagtacat aaacaaaggt gaaaatatac tgatagtgga tgatttcttg gcaaatggaa
    19681 gagctgctct tggacttaaa gatttaattg agcaagcaga ggctaactta gttggagtag
    19741 gaattgtcat tgaaaaagga ttccaagctg gaggagcact tttaaaggct aataatgtaa
    19801 gattagagtc tttggcagta gttgaatcta tagataatgg aactgtaaaa tttaggtaat
    19861 ttatgatatt atatacttgt gtttagcaca tattttaact atgtaattat ggaggatttt
    19921 ttatgagatt ttttatagac acagccaata ttgaggaaat aaaagaagca aatgatttag
    19981 gtgtaatatg tggtgtaact accaatccat ctctaatagc taaggaaggt agagatttta
    20041 tagaagtagt taaagaaata agtgaaatag tagatggacc aataagtgca gaagtaataa
    20101 gtttagaaca taaaggtatg atagaggaag cagaaaaatt atctaagata cataaaaata
    20161 tagttataaa gatacctatg acagcagaag gtctaaaagc agtgaaagta ctatcaagta
    20221 aaggtataaa gactaatgta acacttatat tctctgcggg acaagctctt ttagctgcta
    20281 gagctggagc tacatatgta agtccttttg ttggtagatt ggatgatata tcacaaaatg
    20341 gtcttgattt aattgaagaa atcgttgaca tatttagtgt aaatagcatt gaggctcaaa
    20401 taatagtcgc tagtgttaga aatccaatac atgttttaca agctgctaga atgggagcag
    20461 atatagcaac agttcctctt aaagttataa accaaatgat taagcatcct ttgacagaca
    20521 aaggaataga tagctttatg aaggattggg aaggtgcttc tttaaaattg taattataaa
    20581 ttacgtttta taagtatatt aaagaggtta ttatggaaat tctaagtaat aaggctaggc
    20641 tattttgcct agtcttattt tggtgtataa tatattgaaa catgttattg aaatagatta
    20701 attttttaag tgaggaggcg aaaaatggat aaaatattaa gagagataca aagagaagat
    20761 aaaaaaaatc catatactga ccaagaactt gctgaaatgt taaatgtagc taggtctgaa
    20821 gtaatatcag ttaggaaaaa aaataatatt ttagattcaa gagaaagaag aaaaaaaata
    20881 ttaataaaag atatatcaaa aattgtaagt gaaaatcctc aaatgtctga aagaaaaata
    20941 actgaaatat taatagaaaa tggatataat atatcgagaa gtgcagtgtc aaaattatta
    21001 aaagaggaaa atttaaatca gtcaaagagt gggattaaag ataaagacct agcatcatca
    21061 ggaaagtatg taaaggtgag taaagttgta gaagaagatg gatttgaaga attgataggt
    21121 ataaagggaa gcttgaaaga aaaggtaaat ctagctaagt cagcgataat gtatcctccc
    21181 aatggccttc atactataat atatggagaa acaggagtag gtaaaagtga attagctaca
    21241 tgcatgtata aatatgctat aaaaaacaac atcaaagaag agaacagtcc atttatagtg
    21301 tttaactgtg cagattatgc agaaaatcct aacctattga tagctcaact ctttggagta
    21361 gtaaaaggtg catatacagg agctgatgca aatagagagg gtcttgtaga acaagctaat
    21421 aatggaatat tgtttttaga tgaaattcat agattgcctg ctgctgggca agaaatctta
    21481 ttttcattaa tagatagagg agaatttaga agattaggag aaagtagttc taatagaaag
    21541 gcaaatgttc tcataataag cgctacaaca gaaaatccag attctaactt acttcaaaca
    21601 tttagaagac gtataccaat ggtaataaat ttacccaata tgagtgaaag accaaagtct
    21661 gaaagatatg aaataataat aaagtttttt gaaagagaag caaagcgagt aaacaagaat
    21721 tttataataa ctaaagaagt tatgtgtgca cttatgaatt ataagtgtac tggaaatata
    21781 ggtcagttaa aaagtgatat acaagtaact tgtgctagag cttttagtaa atcgatattt
    21841 tctagcgata aggtaattat agatttagat agtttaaggg attatataaa aagtggatat
    21901 tatgattgta attgtgaaaa tgaaaagtat tctgagtttt cagttgaaga tatatatata
    21961 gatattagtg aattggtgag tggaaaaaat agaattttat ccgataatga gataagtgag
    22021 atatataatt atgcagaaaa agagcttaag gttttggaaa gtaagtgctt ttcagaagaa
    22081 gaattaaaaa atgcatttat agaaaagtta gacaagaagt ttaatgctat taaaaacaat
    22141 aaaaatttat ctgatagaag aagtcaaata gactggggag gacaattaaa agagtctaca
    22201 ttggagataa tggataaggt aattgtattt atcaaggagc agtttaagca tgtaaatcaa
    22261 ggcttgtatt tggctctagc aattcacatt gagcatgcta taggaaggat aaaagatgga
    22321 aaaactataa taaatccgag tttagataaa ataaaagcat caatgccaat ggaatatgag
    22381 ctatcgagat atataatggg tatagttgaa gatttgacac aagtaacatt tccagaagat
    22441 gagcttggat atttggctta ttatataaat aaattttgtt ataatgagga aagtataaaa
    22501 gataaagtta aagtagtaat agttacgcat ggtaaagttg gaattgaaat gtctaaagtt
    22561 gtaaaccata ttttaggtat agaatgtaca ataggtatag aaatagcact tacagattca
    22621 ccatcagagg ggatagaaca tgtattagaa gagcttcaaa agatagaggc tcgaaaaggt
    22681 attctagttc taattgatat gggttctttg gtaattcttg gtgatgaagt tgaaaaaaga
    22741 cttggaatta gatgtaaaac tgtaaataga gtagatactt tactggcaat ggaagcagga
    22801 aaacttgcaa ctattgaagg aaaaagttta gatggtataa tagcagattt aaagaaaaat
    22861 aagaattatg ctatgataaa tacaaataaa tttagttaca gaaaaaacga gtatggaaag
    22921 aaaaatgtaa tcattacttt atgcttgagt ggtgtaggta cagctttaaa tttaaaagat
    22981 catatagaaa aacaaattaa ggaatatgat gcttcaattg aagtaaagcc aattgctttt
    23041 ttaaataata atttggaaga cgagttaaat gatattgaag ctgaatataa tattattgct
    23101 atctgtggaa ctattgatat agattataaa aacgtgcctt ttatttctta taatgaggtg
    23161 cttggtaaaa atggtattaa taaagtatta gagcatttaa atagtaaaca gccagcttta
    23221 aatattgaaa atcagttgaa taatttaata catgaagatt tgattttata caattttgaa
    23281 ggaatttcaa aggagtatat aattgatact cttgtttcaa agcttgaaga aggtggatat
    23341 gttgattcaa agtatatatt aagtgtatat aaaagagaag caatgggaag tgtcgtaatg
    23401 gcctctaagg tagctgtgcc acatggactt ccagaaaacg ttataaagcc tgcaatagca
    23461 attgcaaggt tgaataaacc tatagtatgg gacaataaat ttatggtgga tttggtggta
    23521 ctattggcac ttaaagaaaa taataagaaa gaaatacgaa gcttattttc aaaaattaat
    23581 gaccagcata ctttagaagt tttattaaat actgaagata aaaatgaaat aaaagaactt
    23641 ttaagctaat tattagtaca gagaagaaag acaaaaaatt tgctttagta taaaatttaa
    23701 aactacttaa ttgctaagta gttaattact cgagataagg ctagttctat gaaatttatt
    23761 tatatgattt tatagaatta gcttttttct tggtaaataa aatatatgaa tttatttatg
    23821 caaaaagtaa attttattgg acaattctat gaaaattttt ttctatattt cctataaaat
    23881 atagaatata taaaaaatat gtaaaaataa attaaattca taataaataa ttttataaaa
    23941 tagttgttaa acataaaaaa ccatttataa aaaacaaaaa aatcatttct ctaacaaaaa
    24001 aaaccacaaa aaatataaaa aagtatggct agttaagtga ttaacaagta aaaattaaaa
    24061 taaaaatcaa aaaaaattta tcaatgaaag tcagctaaaa tcctagaaga gagcattatt
    24121 ttaaggaaaa cattttcttt tgtttgacaa aaaaagccac tttttggcac acaacatgct
    24181 ttatataaag tcaagaaaaa ctaaggtaca agacaaaggc aattaggagg tgtaaatatg
    24241 gagtttataa attttgtaag tgataaagtt atagaactta aaatggattg taaaacgaaa
    24301 gaaaatttct ttaaagaaat acataataaa gtttttgatt taggctttgt aaaagaagaa
    24361 tttggagaaa aaatattaga aagagaaaat gtatttccaa caggattaaa tttaggtgat
    24421 tatggagtag ccataccaca tacagatgca gaatatatta aagagcaatt tatagcagtc
    24481 tgtactttta atgaaccagt agtatttagt tctatggaag accaagatga aaaagtacct
    24541 gtaaatttgg ctttcgtatt aggacttaat caaccacaca gtcaattatc tgtattaaca
    24601 gaattaatgg gaattatgca aaataaagag ttagttgata agttgatgag ttctactgat
    24661 aaagaagaag ttttaaagac actaaagtct ttataaaaat aaaaaagtac taaaaataaa
    24721 aaataaaaat tattaggagg aaataaaaat gaaaaaaata ttagtagcat gtggagcagg
    24781 aatagcaaca tcaacagtag tttgtgacaa ggtagagaaa ttagttaagg aaaataatat
    24841 acaagcagaa gttatacaat gcaaaatagc tgaatgtgca acaaagcaag aaggagcaga
    24901 tttaatagta tcaacaacta tattaccaac aacttataat ataccagcaa taaaagcaac
    24961 agcatatata actggagtaa atacagcagc attagataaa aaaatattag atgctttaaa
    25021 ataatcctat catagataca ttgaaataat attttataaa acagaaaaag gaggatttat
    25081 aaatggataa attattagca gtagtacaat acattttaaa tatgggaccg acagtaatac
    25141 taccaataac aattgtagta ctaggaatga tatttagaat taagtttaca aaagctttaa
    25201 agtcaggtct tacaatcggt ataggatttg tgggtataaa cttagttgta aacctattaa
    25261 catcaacatt agggccagcg gctcaaggaa tggttgaaag attcggactt aatttaacag
    25321 taatcgatgt tggttggcca acaacagcat cagcaacatg ggcttcacca gttgcaccaa
    25381 tattaatacc agtttgttta ttagtaaact tactattatt atacttcaat aaaacaaaga
    25441 tattaaacgt tgatatatgg aattattggc actttatagt agcaggtgcg gttggatata
    25501 tagtaacagg aagctatgtt tgggctatat tctgtgcaat attaatggaa ttattagtac
    25561 tatttattgc agaaaagaca gcaaaaaatg ttcaggagtt ttatgattta gaaggtatag
    25621 cattaccaac tggttcaaca gcttcatttg ctccacttgg atatttagta ggtaaacttg
    25681 ttgaaaaaat accaggaata aataaaatac aagcagaccc agactctata caaaaaagat
    25741 ttggtatatt tggagaacca atgatgatgg ggattatact aggtataata cttggagcac
    25801 ttgctggata tgatgtagca ggaatattta atgtaggtat atcaatgggt gcagtaatgt
    25861 tacttatgcc tagaatggtt agaatattaa tggaaggttt aattccaatt tcagaatcaa
    25921 taagagattt cttacaagca aaatatggag acagagaact ttatatagga ctagatgcag
    25981 cagtagcaac tggacatcca gcagttatat caacagctct tatactagtt cctataactt
    26041 tacttttagc agttatacta ccaggaaaca aagtcttacc atttggagac ttagcaacaa
    26101 taccatttta tatggcattt atagtgtgtt ttagaaaagg taatatagtt caatcagtta
    26161 taacaggaac aatagtaata gctatatcac tttatatggc tacaaacttt gcagatgttc
    26221 atacacaatt attacaacaa gcaaatatga tgccagaagg aacaacaatg gttacaagta
    26281 tagatacagg tggaaacttg ctaaaatggg gaatattgaa attatctcaa ttagtttctt
    26341 catttggaat attctaatat gtagttaata tagattactc ttttttagta aagtatgcga
    26401 aaatttaata tttagcaact actaaaaaag agtaatattt tttataaatt tttaaagaat
    26461 acaaaataca agaggaggag atttatcatg aaagcagcag tattacatgg cacaaatgat
    26521 atgagatttg aagatataga aataaaacca tgtgaaagtg atgaagtaaa aataaaagtt
    26581 atggcagcag gaatatgtgg gtcagatcct ccaagagtat taaaacattg gaaatatcca
    26641 gttccagcta taccaggaca tgagttttct ggagtaattg ctgaagttgg taaggatgta
    26701 aaaaatgtaa aggttggaga tagagtagtt gcaattcctt ttataccttg caatgaatgt
    26761 gaatattgta aaagaggatt attttcatta tgtgatgacc atggaatgtt aggagctaaa
    26821 agttttggag cttttgcaga gtatgcaaat ataaaggcta caaatgtact tccaatagga
    26881 gacatggact ttgaagatgc tgccatgata gagccactag cagttgctat gcatggagta
    26941 cttaatatag gtgttcaagt tggagacact gtagcagtaa tgggtagtgg aacaatggga
    27001 cagttagtaa tacaaggact taaaatagca ggagcaggaa caattatagc agtggatata
    27061 tctgataata aattaagaga atcaaaggaa ttaggtgctg atataataat taatgctaaa
    27121 gatgttaacc cagtagaaaa aataaaagaa cttactgatg gaaaaggtgt tgatatagct
    27181 cttgaatgtg caggttcaaa aataactcaa gaacaatgtt tacttataac taaaaagaaa
    27241 agtaagatag gatttttagg aatagcatat tcagatataa ctttatcaga agaagcattt
    27301 gaaaatatat ttagaaaaga actagagtta aaaggttttt ggaactctta ttctgcacca
    27361 ttcccaggac aagaatggac taagggaatc aatctagtta atgaaggaaa gataaaatta
    27421 aaagaaatgg tatctcatag attctcactt gaagatactt acaaagcatt tgaaatgata
    27481 agagatagaa aagaagaatt taataaaata ttaattttac cacaaggggt tgaaaaataa
    27541 tgaaggcagt agttaaaact aaaccaggtt atgataatgt agaagtttta gacgtagaag
    27601 aaccaaaagc tacaggagac aaggttaaaa taaaagtaga atatagtggt atatgtggtt
    27661 cagatataca ttcatttaaa ggtgaatatg caaatataaa agccccagta acattaggcc
    27721 atgaattttc tggaattgtt gtagaagtag gagaagatgt taaaaatata aaagtaggag
    27781 atagagttac atcagaaact acttttgcga cttgtgaaaa atgtatatac tgtgaaacta
    27841 aagattataa cttatgccaa actagaaaag gtattggtac tcaagtaaat ggaagttttg
    27901 ctgagtatgt actatcaaga gaagaaagta tacatgtact tcctgaaaat gtatctttat
    27961 tatcagcagc ccttacagag ccactagctt gttgtgttca tgcagctctt gaaaagacta
    28021 ctatagaaag ctctgataca gttttaataa taggaccagg accaattggt ttattattag
    28081 ctcaagttgt taaatctcaa ggagctacag ttataatgtc tggtgttaca aaagacaagg
    28141 aaagattaga aatagctaaa aatcttggag tagatagaac tgtaaatgta atggaagaaa
    28201 gtttggcaga tgtagtgaat gaaatgactg atggaaatgg agtaaacaaa ggatttgatt
    28261 gttctggatt tatgccagca gttaacgaag ctttaagatt gcttagaaaa aaaggtactt
    28321 tcgtacaagt tggaatcttt gctaaaaaat taaatgaaat ggaccaagaa gcaataatcc
    28381 aaagagagtt acaatatata ggaagtagat ctcaaaagcc aagttcttgg attacagcat
    28441 tagatttaat ggcaagtgga aaagtaaata cagaagcttt agttactaga actgttggtt
    28501 tagacgaatt taaagaaggt attacagctt taatgaacgg ggaagaaata aaggtagcag
    28561 taaaatctta aaaaaacatt tgtattattt taaaaaaaat tgtaaaatct actgtaggat
    28621 aaaaaatgta agatcttaaa aaataacagg aggaagaaag tatgagcact cttagagatc
    28681 attctaataa tattagaaaa aacataataa aaatggttgc agaagctaaa agcggacatc
    28741 caggtggttc attatcaata gctgatatgt tgacagtatt atattttgat aagatgaacg
    28801 tatgtgcaga aaatccaaaa atggctgata gagatagatt tgttctatca aaaggtcatg
    28861 cagcaccagc actatatgct acactagcag aaaaaggatt tttcccagaa gaagaattaa
    28921 taacactaag aaaatttggt tctaagttac aaggtcaccc agacatgaaa aaagtagcag
    28981 gaatcgatat gtctactggt tctcttggac aagggctatc tgcagcaaat ggtatggcac
    29041 ttgcaggaaa attagacaac aaagactata cagtatatac tatattaggt gatggagaaa
    29101 tccaagaagg tcaaatatgg gaagctgcaa tgacagcatc acattacaaa ttagataatt
    29161 taattgcatt tgttgactta aatggattac aaatagatgg ttctaatgaa gaagtaatga
    29221 atgtaaatcc agtagatgac aaattcaaat cttttggatg gaatgtaatg gtaataaatg
    29281 gacattcttt tgaagaaata ggaaatgcag ttgatgaagc taaaaaagtt acaggaaaac
    29341 caactgtaat aatagctaaa acagtaaaag gtaaaggtgt ttcatttatg gaaaataatg
    29401 ctgcatggca tggaacagct ccaaatgcag aacaagcaga gcaagcattg aaagatttag
    29461 aaggaggtct ataggaatgg gaatagcaac tagagaagct tacggacaag tgttaaaaga
    29521 acttgcagaa aataaagata tagtagtatt agatgcagat ttaggaaaag ccacaaaaag
    29581 tatatcattt aaagaagttg ccccagatag attttttgat atggggatag cagaaggtga
    29641 tatgattggt acagcagcag gtcttgctac ttgtggaaaa ataccttttg caagtacgtt
    29701 tgcaatattt gcagcaggaa gaggctatga gcaaataaga aactctgtag catatccaaa
    29761 tttaaatgtt aaaatagcag ctactcatgc aggtgtaaca gtaggtgaag atggtggtag
    29821 ccatcaagct atagaagata tatctctaat gagaggtata ccaaatatgg ttgtattaaa
    29881 tccagcagat gctttagaag caagacaagc tattcttgca tctattgatt ataatggacc
    29941 tgtttatata agattaggta gagctgcaac tcctgatgta aacagtgaaa attataaatt
    30001 tgaaatagga aaaggaactg ttttaagaga aggttctgac ataacagtga tagctacagg
    30061 aataatggta gcaaaagctt tagaagcagc agaagaactt gctaaggaag gtgtaaatgt
    30121 agaagttgtt aatatatcta caataaaacc attagatgaa gcattaataa aagaaactgc
    30181 taagaaaact ggaaaagtag ttacagcaga agaacacagt ataataggtg gtttaggttc
    30241 agctgtatgt gaagctttag cagaaactaa agatgtagta gttagaagaa ttggtgttaa
    30301 agatgtattt ggtcaatcag gtacaccagc tgatttatta aaacactatg gcttaactac
    30361 agaagatata gtaaaaaata taaaagaatt gttatagata taaatatatc taaataacaa
    30421 attgatttga cataaatcaa ttaaagctgt cttaaaaata gaaagaaatt tctacaatgg
    30481 gacagcttta tttttacaat actttatttt tatgataatt gtaaaatagt ataaaaataa
    30541 tcaaaaaaat tatagaaaga ggtgctattt aatgaaaata tctattggat gtgaccatgg
    30601 tggttatgaa ttaaaggaat atataaagga aaacttaata aataaaggta tagaaattga
    30661 agatgtgggt acaaattcaa aagaaagcgt agatttccca atatatgcaa aaaaggtaag
    30721 tcaaaatgtt caaagtaaaa aatctgattt aggtatactt tgttgtggaa caggaatagg
    30781 tatgtctatt gcagcaaata agtttaaagg aatcagagca gctgttgttt ctgactgttt
    30841 ttcagctaag gccactagag aacacaataa tacaaatgta ctttgtttag gggaaagagt
    30901 tattggaaaa ggattagcaa tgagaattgt tgaagaatgg ttaaattcaa gttataatgg
    30961 agaaagacat gcaagaagat tacaaatgat tgatgatata gaaaaagaaa atttataaat
    31021 atataattta taataagggg gaacgttatg tctattattt gtgcttcaat aatgtgtgca
    31081 gaccaaatga agttaaaaga ggaattagaa gctcttgaag aagcaggagt aaaattactt
    31141 cattgtgatg ttatggatgg aatatttgtt aaaaacttgg ctatggggcc agagttatta
    31201 aaatcaataa gtgaaaatac aacaatacct ttagacatac acttagccac agaaactcca
    31261 gataagtata ttgatatgat gtcttatata aagccaaaat atatttcttt tcatgtagaa
    31321 tcaagcacaa atgtaagagc tgatatacaa aaattaagaa attatggtat agggccagta
    31381 ttagcaataa gccctcaaac aagtgtggat aaaatagaag aatatatagg tttagttgag
    31441 ggtatcttag taatgacagt aaatccagga tttgcaggtc aaaagtttaa tctttctgta
    31501 ttggataagt tggataaact tacagagata ctaaaagatt atgataatcc tccatttata
    31561 gaggttgatg gaaatataaa taaggatact attaaactta tgaatggtaa aaaagtagat
    31621 atatatgttg ttggtacttc agctttattt aatgacaagc ctccaatttc atataaagat
    31681 aaaataaaag aactaaaaga aagtataaag taactatgta ataaaattat aatatgtttc
    31741 tatttaatgg taaaatttta ggtagaaaca agaaattttg taatatttaa taaaaatttt
    31801 ggttggataa ggagagtttt ttatgggaga cctatataaa ctgcaaaatg gtactgatat
    31861 tagaggaata gcatatgaaa atgattctaa agaagttaac ctaactgtag aagaagtaag
    31921 aaaaatagct aaagcgtttc atatttggct gaaagaaaag actaagaaag ataaagtaac
    31981 tgttgctata ggaactgatt ctaggataac aggaagccaa tttagaagtg ctgtcattga
    32041 aactttaact aatgatgatt gtaatgtaat tgattgtgag attgcaacaa ctccagctat
    32101 gtttatgaca actattatgg atggatataa ttctgatggg tcgattatga ttacagcaag
    32161 ccacttacct tattactata atggtttaaa gttttttaca gaaagtggag gcttagaaaa
    32221 agctgatata aaagaaatgt tggatatagc tgataaaaat gatagtgtag actatgagaa
    32281 agcaaataaa aaaggtaaag ttatatcaaa aaacttaata gaagattatt ctaatttatt
    32341 aatagataaa attagaaaag gtgtaaattc aagtaaaaat tatgaaaagc ctttttctgg
    32401 acttaagata ttggttgatg caggaaatgg agctggtgga ttctttgctg aaaaagtttt
    32461 acacatactg ggagcagata caacaggtag tcagtttttg aacccagatg gtatgtttcc
    32521 aaatcatata cctaatcctg aaaataaaga agcaatggaa agcatatgta aagctgtgtt
    32581 ggacaataag agcgatttag gaataatatt tgatacagac gtagacagag ctgctattgt
    32641 aggtaaaaat ggaaaaccaa taaataaaaa tgctcttata gctgttatat catcaatagt
    32701 attagaagaa catccaaaaa cagctatagt aacagattcg attacttctg agggacttgc
    32761 taagtttata aatgaattac aaggaagaca tcatagatat aaaagaggat ataaaaatgt
    32821 aataaatgaa gctataagat taaatagtga aggtgaagag tgccatttag ctatagaaac
    32881 atctggacat gctgctttaa aagaaaatta tttcttagat gatggggcat atttaatagc
    32941 taaaatttta ataaaagtag caaaactatc cttagaagga aagactatag aagaattaat
    33001 agaaaacctt gaagaagctc gagaagaaaa agaagttaga ataggcatta ataaagaaga
    33061 ttttagacca tatgcagaag atatattaaa agatttagat tctcatgtta ggagtattga
    33121 tggatggtct gtagcaccaa ataattttga aggtataaga gttaattgtg atgaatcaaa
    33181 tggagatgga tggtttttac ttagaatatc actacatgaa ccattattag ctttaaatat
    33241 tgaatcaaat aaaattggtg gagcagaaca aatttattct aagttaaaac ttttcttaga
    33301 aaaatatgat ttaagaggtc tttagggtaa atagaaagaa tatatttata tcgaagaata
    33361 tatgtaaatt tgtacctaag attacaaata ttacaaaatt gtaatcttag gtaacagaga
    33421 tgtaactgat tatggaaaca ctatgtgata ttataaatac agagagatga aatacatgct
    33481 gaattataga gaaatataat ttaaaatgta ttaatatcac accaaaaagg tataatcaaa
    33541 aaatatgtaa atataaaccc caacatttag aaaaagacct cgaaggaggt ttttttcttg
    33601 tttaaataac ttaaaatata tttttatatt aatgtatcaa ttaactcttc taaaagtaag
    33661 cattacctta aataaatcag catctataat tatttccatg tctgcatttt ctaattcaat
    33721 tatattttga gcaatggata aaccaagacc agagccatct gtttttctag atacatcacc
    33781 ccttctaaat ctttctacta actcttctgg ctttaggttt agtttatcat ttgaaatatt
    33841 tttaaatgtt aatattatat tttcttcctt tataaacata tctatataga ctctagtatt
    33901 aggcatagag tattttagta tattgcaaat tagattttca aaaactctcc aagttttttt
    33961 tccatctatc atggtaaaaa cagcatgctc aggtgtttct attataaaat ctaaattgct
    34021 attttgaatt ttgtcttcaa actctacaat actttgaatt aaaagttctc ttatatctgt
    34081 ttttgttata ttaaattcta catctccact acttagctta gatgcttcaa atatgtcatc
    34141 tattaataat tttaatcttt gtgatttttt atctaaaata tttatatagt catttacagt
    34201 ctctttatca aagtcattat tttttaacat atcaacatag cttaatatag atgtaagtgg
    34261 agtttttaag tcatgagata cgtttgtgat aagttctgtc ttcatccttt cattttttat
    34321 actagaatct acagcatttt caagaccatc tgttatatta tttatacttt tagataagtc
    34381 agatatttca tcatttccta ttacttctag cttatacttt aaatctccat tctcaatatt
    34441 ttttacattc tttgatatat tatttatgta attaaactta gcagataaaa agttgtatat
    34501 atataaatga aaaactgtgt ttacaagtgt aaataaaatt actatagcta ttgcctcagc
    34561 gtaatatata tacataaaga atatcaaaac catattttgg ataatagctt gcataactat
    34621 agatattaaa aatactatag ctaatttttt atttatagtt tttctattat acacactaag
    34681 taagtttgaa aagaatacat atgttataga catatctttt agattatagt tattatattt
    34741 aatattttca tatattatgt agactataga tataattata aaattgtaga ttattagatt
    34801 agtgttgtaa aacattttat tacttgtaaa ggcatgaaac cttaaaatta atagtatcaa
    34861 aattaatata agtgtaagtt ctatttttac cttagaaaaa aggcttatga cattctctaa
    34921 gaatttgact ttagtctgtt tatttttttt atacaatata aaacatagta caatcaataa
    34981 aaaacttgat aatataacac ctatacagac aaactctaat tctacttttg acaaatgtaa
    35041 aggagtgcca aaaaaataaa aataaggtat agtaaactct ttataataat aattaatatt
    35101 ataaaaaaag ttattattca aatcataaac tgcttcaaat aatatagaaa ttaatagaaa
    35161 cagtagtaat aataaaataa attttgcttt agaatcatta ataattaagt ttttcaattt
    35221 tgtaaccaat gccccacacc acctttaaat attttggttc ttttggatta atttcaattt
    35281 tttctctaat tcttctaata tgaacagcaa tagttctctc acaactataa gcatcctcat
    35341 tccagacatt ttcataaatc tcacgagaag agaaaatttt accttttcgt gacataagaa
    35401 gagtaagtat tttatattca agtggtgtca ttttaatttc ttccccatct aaagaaacta
    35461 tttttgtatc tgtatttagc tctagtccac cactttttag aatataatta gtatcatttg
    35521 agttatttgt attactaaaa tcacctaaat ttatgtatct tctaagttga gattttactc
    35581 tagcagtaag ttctaatgga ttaaatggtt tagttatata gtcatcagca cccatattaa
    35641 gtcctataac tttgtccata tcttctgatt ttgcagataa gagaatgatt ggtatatttc
    35701 ttttttctct tattttaagt gtagttttaa gcccatctaa attgggcatc attacatcca
    35761 ttattattaa tttaatatca ttttcgatta gtatatccaa tgcttcaagt ccatcatatg
    35821 cctttaaaac attaaagtta tcaggtttta aatagaattc tatagcatca actatctctc
    35881 tatcatcatc tacaaccaat atattttgat tcaatttatt aacctccttg aaattttatt
    35941 tttaaccaat ctttatttta taccaaaaag aagtattaga aaacagtaag catatttgtt
    36001 atgaaataaa aactaatact tttaatatat tgtcaatgca tatagtaatt tttaaagcat
    36061 agatgtatat attaatatga agttaaaagt ttatgaaaat atctaggata atttatcaca
    36121 ctccttatgt cattttatgt ttattaattt tttaggtcta aacatatact aacaaatata
    36181 aataacaaat gagtttcata attattaaga atttataaca aatatttatt ttgatgtatt
    36241 ttaatataaa taggtagttt aatgtagata tgcataaaac ctttaagaag aaaacttata
    36301 ggtttcaata ataatatttt tatatatttt aagacatgat gaatctagat atggggaata
    36361 gttggatgag aaaaagtagt atagcacaaa aaagtgctat atcacaatct caaataattt
    36421 actttttata atttaagcta aaagaataaa tatttcttat aaattaggtg tatgaagtat
    36481 ggcaggaaca tctttattcc ttaataattt atcaaaagat ttatgatcag atttataacc
    36541 aataccttct ctatcttcta actcatcaac aattttgtat tgttgacagg cattttttac
    36601 cttttcatag tcttcagtag aatgagcttt ataaactatt ttattattaa aagttatttt
    36661 atcagcaaat tttggtttta caaaatattt ggcatctttt attttaccag cattatataa
    36721 attttgtata acagaaccat tttttaaatg ttgcaaatgg tcgtctaact tataagtatg
    36781 tgctatatga gttacatttt taaaattttc ttcaaagtta atggcaattt tttccatgta
    36841 attgaagtca gtattaccat ttgtatttgt ttctggtaat aatattatat tctccctcct
    36901 tacaccaagg ctattaagag aagctaaaaa ttctctgttt ctatattctg atttttgtat
    36961 atctgttaga ttttcatact ttttatattt atcaaataca gatatgccac atccagtaga
    37021 taccaaaacc acaaatacat gattttttcc tctttctttt atagcattaa caatagcact
    37081 cccagcccac aacacttcat catcttgatg ttgaacatag aagacaacat aatctttaaa
    37141 ttttttatga ctataattca tatagctgct aaaagcagtt aaaatcaagc ataaagctag
    37201 aatgatagat aaaattctat atttacttat agtaaatttt ttattcttaa atgtcattta
    37261 atcatctcct gaaaagttgt cttagttata ttatcaaaca aagtgctaaa aattatttct
    37321 taaatactga aaaatataga gtagattaat ggaataaaat ttttttatta atttaaaatt
    37381 aagaaaatat ttatgtataa aaccaatcaa aatattaatt agtgatagga attgagattt
    37441 aaagggattg tacatttatt tgagattcat ttatatagca ttactttagt attgagaata
    37501 aaagtaataa ctatttaaat ttaaagagta cacattgata tttgtgtact ctttaatgat
    37561 gtgattgctt atttactaca aaaacatagt ctttttaaac ctaaaatatt tgatgcacca
    37621 gctatcgtag tgccataata gtaaactggt ttattaagtg atagaaaatt tataattgag
    37681 ttattacata atgtacttac tgtgcaaatc actaaatcag cccaagatat tacatcttca
    37741 taatctcgta taccatcttc aatcttaaca ttatactgaa tagtatctac aaattcagga
    37801 tttaaatcta atactcttgt ttcgaaaaag tccttaatat tatcaattat ggctggttga
    37861 tatcctataa ttgctacttt tgggttatca aattcccttt ttataaactt agggaagttt
    37921 tttgcacata tctctggttc attattttta caatgaatcg ttctatccgt cttacctaag
    37981 tatttcataa cagcatttaa tgatgctata aaaagacttc tgtcatgagg attatcatat
    38041 aaatctaatg ataatatatc ttttaaagaa ccttcaaacg tagatggagt agaagtaaat
    38101 gcttgtcctt tagctccttt aaagtcagct tcaagcatta tttctttacc aattataatt
    38161 ggaaaatctt tacgcttagt atttccaatt gcttcttttg aagatagcga tttggataca
    38221 atacttatca tatccatatt taaattatat ttatctactt gtactaatag ataatctctc
    38281 aaaatatcaa atatattcat agaaaaaaga ttctccttta tgttttattt cttcatttaa
    38341 atcaaaaata tgatttattt ttccattgtt cattattcct atcttatgag caaaaaacat
    38401 tgcttcagaa atatcatgag taacatgaat caccgttggt ttgtatttta tgtagacatc
    38461 cttcaaaatt ttgcttatat gtttttttgt aatataatct aatgaactca taggttcatc
    38521 taaaagtaat atatcaggtt cagctattaa agcacgtgca aaagcaactc gttgcttttc
    38581 ccctccactt aaatttttag gatatctatt ttttatatcc tctatctgca ttaatttaga
    38641 caagtggtta actttaagaa tctgttcatc tataggtatt tttcttaatt taagtggaaa
    38701 gcttatatta tcttcaacat tcatatttgg aaataattca aaattttgat acacaaatcc
    38761 tatatttcta ttttctggag gtaagcttga tattaaattt tttttaaatg atacacttcc
    38821 tttagcagat ggatatcttc ctgctaatgt ttctagaaat agagttttac ctgagccact
    38881 agatcccaat aaaacaaagt attcatggtt attaatattt aaactatttt cttgtaattt
    38941 aaaattaggt aaatcaagag ataaatttct tatttttaac atattaattt ttatcaacct
    39001 ctctattaaa aaatatctca aaaagcatca agcatgtaag tgagataaat atcaaaatta
    39061 cagctattcc aattgcaata tctatatcac cagtagacat atttaaatat atagatgtag
    39121 gaataatttc agttttcata cgtgtagaac ctgctatcat agctactgca ccaaattcac
    39181 caagtgctct tgaccaagtc attacagttg cagatatgat tccagttttt agatttggaa
    39241 gaattataaa tttaaatgct tcccaactat tacatccaag agtacgtgca acaaatatca
    39301 tcttttcgtt actttcattt agtgaagtgt gaagtatttt aatagtaagt ggaagattta
    39361 caaatacttg tgccaaaatt ataccttgct ttgtaaatac aaaatcaagc ttaagaatct
    39421 tgtaaatgga atctccaatc cccattcttc caaacaaaag caagagagca attcctgata
    39481 ctaggtgggg aagagacata gggagattta ttatctgtat aatcagtttt ttaaatggta
    39541 atttagtaat atgtaaaaag tatgctactg gaatagctaa caaaaggcat actatagtag
    39601 atattaaaga tgttttaata ctaagtgtta ttgcatactg tacctccact gattttaagc
    39661 atataggaac ataacttatc cctttaatga ttaacataag aagtggtgat acaataaata
    39721 aaaagactac agcaaaaaat ccagtaagta gtatagtaaa aaatttattt ttcaagttta
    39781 atcactactt aacaggttca aaaccatatt ttttaaatat ctgtttagct tcatctgatg
    39841 ctatgtagtt tataaattta ttagctaaat cttttttatc actatagttt acgattcctg
    39901 ctgatatgag ttggtctatg tttttatctt gagcaatttc tataacttct acatcctcat
    39961 tgccaaagac tgaatctttt gtggctattg tagcatctgc tttacttgat gttaactgta
    40021 caatcatttc atttacagtt gcagctgtag agacaacatt atcatttata gattttaaat
    40081 tatttttttc aagtattttt tgcgtagtct tacctatggc attagactct ttgtctccaa
    40141 aaataagttt aactccatct ctatttaagt cttccagttt ttgaatatta gctggattcc
    40201 ctttaggtac aactattgca ggagtatgat gtgcaactgt tttatgttca cctacaagtt
    40261 ttttttcaaa ggcctttgag taaattggtt cagaaccaac aataaatgca tctcccttgt
    40321 gacttgtttc aatttgagct attagctgtg cagagcctgc atagctatat tcaattttga
    40381 tattattttc tttctcaaat ttttgagcta tttcatccat tggtttcttt aaacctgcac
    40441 ctgaatatac aaaaagagtt tctttttctt ttgagtcatt agctgttgtc actttagcat
    40501 tagattcatt attcgctttg ttttctttag aattacatgc agtaatactt aatgaaataa
    40561 tcatagataa aattagtact gaaaattttt taaacataat atattccccc ttggtaaatt
    40621 agtgtaaatt ctttttttag tatatcatac taagaaaacg atgtctaagc ttttgttatg
    40681 ttttataatg ttttgttatg ttttgtcatg tgtggttaag agaaaatcta tctataatgt
    40741 cattttcata gagagttatt tcatttttaa cttttattaa tactttaaag tattttaaat
    40801 ttaaaaacta tttttaaatt aatcgattgt agtc
//
LOCUS       DAFNNB010000018        38651 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0018, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000018 DAFNNB010000000
VERSION     DAFNNB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 38651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38651
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0018"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(131..325)
                     /locus_tag="L3M60_000829"
     CDS             complement(131..325)
                     /locus_tag="L3M60_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225523.1"
                     /translation="MFQVINFLCLFCVGFLVLDVMIYFCKDAMDIFENTTNVNKVKSI
                     NVWADRVRANSYSYENKIAK"
     gene            complement(634..2163)
                     /gene="lysS"
                     /locus_tag="L3M60_000830"
     CDS             complement(634..2163)
                     /gene="lysS"
                     /locus_tag="L3M60_000830"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBN6225524.1"
                     /translation="MKNNQQSNEEAQIQEDLSEVLQVRRDKLKKLQESGRDPFKESRY
                     DRTHYSMEIKDNFESLEGKTTKIAGRIMSKRIQGKAGFIDIQDQEGRIQSYVRLDAIG
                     EEEYSVFSTYDIGDIVGIEGEIFKTKKGEISVKAKSVVLLCKSLQVLPEKYHGLKDQE
                     LRYRQRYVDLIVNPEVKNAFLIRTKALKALRAYLDDRGFLEVETPILNTIAGGANARP
                     FITNHNTLHIPMYLRIANELYLKRLIVGGFDKVYEMGRMFRNEGMDLKHNPEYTAIEL
                     YQAYADYTDMMEITENVIAHMAEVATGSMIVNYQGTEINFTPPWKRMSMEDCVKEYSG
                     VDFSTINTDEEALEVAREKGIEIKPGMRRGEVINAFFEEFGEDKLIQPTFITHHPVEV
                     SPLSKRNAEDPRRTDRFEVFANKWELANAFSELNDPIDQKGRFIDQLRKRELGDDEAF
                     EMDEDFLKALEVGLPPTGGLGIGIDRVIMLLTNSPSIRDVLLFPTMKPIDNNPNKEEE
                     N"
     gene            complement(2178..2657)
                     /gene="greA"
                     /locus_tag="L3M60_000831"
     CDS             complement(2178..2657)
                     /gene="greA"
                     /locus_tag="L3M60_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBN6225525.1"
                     /translation="MEENKEFLLTQEGYDKLEEELENLKVVKRKEVAERIKVAISFGD
                     LSENAEYDEAKKEQAQVEERILKLENMVRKAVIIDESKIDLNVVTIGSIVKVKDLEFD
                     EEVEYTIVGSTEADPYDGKISNESPVGKALLGRAAKEVVEVQVPDGVAKFEILEIRR"
     gene            complement(2766..3734)
                     /gene="dusB"
                     /locus_tag="L3M60_000832"
     CDS             complement(2766..3734)
                     /gene="dusB"
                     /locus_tag="L3M60_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBN6225526.1"
                     /translation="MKIGNLELKNKVFLSPMAGVTDLPFRLICKEQGCGLLYTEMING
                     KALCYDDENTKKMLKIEDEEHPVAVQIFGSEPEFMGRAAEIMNDYSNEILDINMGCPA
                     PKVVKNGDGSALMKNPKLAEEVLRAVVKNSKKPVTLKIRKGWDDNSVNAVEIAKIAED
                     CGISALAIHGRTREQFYTGKADWDIIAEIKKNLSIPVIGNGDVFTIEDSINMLDKTGC
                     DAIMIGRGAQGNPWIFKRINHYMNTGEILPEPTLNEKISTAIKHLKLAVEEHGEYVAV
                     REMRKHIAWYLKGLRNSAKLRDEINKIEDYQEVVSKLEYYMEDSLT"
     gene            complement(3871..4641)
                     /locus_tag="L3M60_000833"
     CDS             complement(3871..4641)
                     /locus_tag="L3M60_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HBN6225527.1"
                     /translation="MLLVFDVGNTNMVLGIYKGDKLVNYWRIKTDREKTSDEYGILIS
                     NLFDYDNVNISDIDDVIISSVVPNVMHSLENFCIKYCKKQPLIVGPGIKTGLNIKYDN
                     PKQVGADRIVNAVAGIEKYGAPSILVDFGTATTFCAISEKGEYLGGTIAPGIKISSEA
                     LFQSASKLPRVELAKPGMTICKSTVSAMQSGIIYGYVGLVDKIINMMKKELNCDDVKV
                     IATGGLAKLIASETKSIDYVDGFLTLEGLRIIYEKNQE"
     gene            complement(4660..5250)
                     /locus_tag="L3M60_000834"
     CDS             complement(4660..5250)
                     /locus_tag="L3M60_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBN6225528.1"
                     /translation="MMNTSASVRKVNVRKMTIIGVLSAISIMLSMTPLGFIPVGPTKA
                     TIMHIPVIIGAIMEGPIVGGAIGFIFGISSLLNAIINPTVTSFVFINPLVSILPRVMI
                     GVLAYYVYQLIIKATNKVYISGLITGAIGSLLNTAGVLGMIYVLYADKYLHAMGKSGS
                     AGKVIMALAAANGVPEAIVGALVVAAVATVLKKSKK"
     gene            complement(5444..6403)
                     /locus_tag="L3M60_000835"
     CDS             complement(5444..6403)
                     /locus_tag="L3M60_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HBN6225529.1"
                     /translation="MYNNILDIEGLKVGQVEDRNGLTGCTVVICEEGATCGVDVRGSA
                     PGTRETDLLDPVNMIQKVHAVVLAGGSAYGLESTCGVSRYLEEKGIGFDVGVGKVPIV
                     VGAVLFDLGVGNFKCRPDLKMGYRACEVANIDILNQGNYGAGCGATVGKIRGQEYAMK
                     GGIGSYSIKLDNGLVVSALIAVNALGDVYENGKVIAGALNDKKTETIDTYKLMKQGES
                     KGGFSVDNTTIGIIATNAKLTKAGCKKISQMGHDGYAKAIFPIHTPHDGDTIFTISTG
                     EIEADITLVGSLAVEVVEKSIINAVKKADSVENIMAYKDIVKK"
     gene            complement(6396..7373)
                     /locus_tag="L3M60_000836"
     CDS             complement(6396..7373)
                     /locus_tag="L3M60_000836"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HBN6225530.1"
                     /translation="MRDKIVEIMLNNKSEFISGEELSKQLGVSRAAIWKHMKALKEEG
                     YNIESVNKKGYRLAENPTDLLSPQNICYKLDTEFIGKNIEHLETIDSTNEHAKRIAVN
                     SLDGTVILSEHQDMGKGRLGRRWDSKPHEGIWMSIILKPDMEPFKAPFITLIAGASVT
                     KALNNMGANTLIKWPNDVILNGKKICGILTELSAEIERTNYVVLGIGINVKIMDFEQE
                     IAEKATSLYKEGYMLSRVDIVRNVLTEFEKLYLDYTVNNSKEKTLKICRDYSAIIDKN
                     IYVLKGDNKELVKCLDINEEGNLLVQRSDGNIKEIISGEVSIRGEKGYV"
     gene            complement(7687..9657)
                     /gene="ftsH"
                     /locus_tag="L3M60_000837"
     CDS             complement(7687..9657)
                     /gene="ftsH"
                     /locus_tag="L3M60_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBN6225531.1"
                     /translation="MNKLLKGAGFYLLVFIIIVGIVQFSGKPTEKIKDLKFSEVYREL
                     TDENISRLYFVNQTSVEGTIKDTNTKFKSYVPTEVMGNKLADEVLDQAKAGKLTFGGE
                     AKPSTPWFVEMLPTLLLIFFMVIIWFVFMNQSQGGGGKVMSFGKSKAKVHKDDEKTRV
                     TFKDVAGLDEEKEDLQEVVDFLKNPKKYIELGARIPKGMLMVGPPGTGKTYLSRAVAG
                     EAGVPFFSISGSDFVEMFVGVGASRVRDLFEQAKKSAPAIIFIDEIDAVGRKRGAGLG
                     GGHDEREQTLNQLLVEMDGFGVNQGIIIMAATNRPDILDPALLRPGRFDRQVVVGTPD
                     VKGREAIFKVHSRNKPLSDDVKMDVLARRTPGFTPADIENLMNEAAILTARKREKKIK
                     METIEEAITKVIAGVAKKSKVISEKERRLTAYHEGGHAVCAHVLEEVSPVHQVTIVPR
                     GRAGGFTMQLPVEDKFYATKNEMKENIVVLLGGRVAEELVLKDVSTGASNDLERVTAT
                     ARSMVTKYGMSSKLGPMSFDSDDEVFLGNSFSSKRNYSEEVAFEIDQETKRIVDGAYD
                     KTRSILQENMDRLEYVAQALLIYETLDAEQFVKAFNKELPLNEIENAVTEENSSKEVE
                     EQLTIKLEKDEEERNNVIDINKNLEDKSDKDK"
     gene            complement(9691..11079)
                     /gene="tilS"
                     /locus_tag="L3M60_000838"
     CDS             complement(9691..11079)
                     /gene="tilS"
                     /locus_tag="L3M60_000838"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBN6225532.1"
                     /translation="MIFDKVLSTINKHNLIQKGDKIVLGLSGGPDSVCLLHVLNRLKK
                     DFNIEIYAAHLNHQIRGIEAQKDALYVSKLCEDMGIIFFVKSINVPKYCENEGLSLEE
                     GARKLRYEMFYEIKDKIKANKIAIGHNLNDQAETVMMRIMRGTGLKGLKGIDYIRDNC
                     IIRPILDVERSEIEEYCEAYNLNPRIDKTNLENIYTRNKIRLDLLPYMKDNFNSNVIE
                     SIVRMSNSLKSDNDYIEKEAEAKFREVSNIKEKGFVEINLDDFVCLHDAIKVRVLRNS
                     IKHILGDTNFVDQRHIEDIMSLEDNSKVNKMLTLPRNIFVYRKKDSIILTNEEIVSEE
                     IEFYYNIPSNGFIKIKELKQIIETQVMSIDRYKSMKLDNSSKGFDFNKVKGGIVIRSR
                     RQGDKIKLAMGSKKVKDLFIDLKIPREERCKIPIITDSEGIICVGDYKISENYKIDEN
                     TKEVLKINFNKL"
     gene            complement(11262..11690)
                     /locus_tag="L3M60_000839"
     CDS             complement(11262..11690)
                     /locus_tag="L3M60_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HBN6225533.1"
                     /translation="MEEKSLSVKISINTRQYDEKGNMDTIEMTAFGKIFYKNDGIYVV
                     YKEKEENIEITNTIKILKNEVSIKKFGAINSTMLFKCGESSTTKYVTPQGTLLIDIDT
                     RKLDINIQEGEHIKLKIDYNINVQDLFLGRNKIDIYIDTK"
     gene            complement(11707..12513)
                     /locus_tag="L3M60_000840"
     CDS             complement(11707..12513)
                     /locus_tag="L3M60_000840"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBN6225534.1"
                     /translation="MSNKPIGVFDSGLGGLTVLKEIMKILPNEDIIYFGDTARIPYGS
                     RSKETIIKYTFQAINFLKTKGVKAIVIACNTATARSLKEAQEKYDIPIIGVIEAGART
                     AVSSTKNKIVGIIGTEGTISSKAYNLEISKIDESIEIVNKACPLFVPIVEEGWANTEV
                     AKLTAKIYLQELKEKNIDSLVLGCTHYPILKRTIGEEVGEHIKLVNPAKETAKDLKKV
                     LEVKNIINNTEIHGTYQYYVSDIPEKFTDIAREFLKKKIDRIQKVEIQKY"
     gene            complement(12715..13200)
                     /locus_tag="L3M60_000841"
     CDS             complement(12715..13200)
                     /locus_tag="L3M60_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HBN6225535.1"
                     /translation="MATLLALTFIFMPIKEVFAYEPTAKIEKINKQEKNIAKKEVINL
                     SDNDLMLLSKLVAGEARGESYEGQVAVAAVVINRVLDPRFPDTIEEVIYQKNAFSVVL
                     DGSIKMAPTDSARKAAQEALYGTDPTNKAVYFWNPEIATCKWINRLNPYMRIGNHVFA
                     K"
     gene            complement(13251..13910)
                     /gene="spoIIR"
                     /locus_tag="L3M60_000842"
     CDS             complement(13251..13910)
                     /gene="spoIIR"
                     /locus_tag="L3M60_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HBN6225536.1"
                     /translation="MRKIKIRLGLLILSLISVISIMTIVINGEVKKVDNISKDYKDKL
                     IRFHVIANSNTDEDQELKLKVRDEVIKYLQPKLQNSKSIKESEAIIKKEYSSLEEISK
                     NIILENGYNYSVKVGIQYSNFPTKQYSNIVLPAGEYKALKIIIGKGEGKNWWCVMFPP
                     LCFVDESNGVIDKSTDDKLKEVLTDKEYKLIKQDTPKKTSRVKIKFKVIEVVKDLEKK
                     F"
     gene            complement(14056..14772)
                     /locus_tag="L3M60_000843"
     CDS             complement(14056..14772)
                     /locus_tag="L3M60_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6225537.1"
                     /translation="MLLIAVKKGLKFVENLTKLNLDNYKPLRDVVFENLREAILEGKL
                     KPGQRLMEVQLAEQLGVSRTPVREAIRKLELEGLVVMLPRKGAYVANMSLKDIMDVLE
                     VRASLEGLAAYLAAERISEEDIKKLKDISEEFKKSTLESDVDALLKLDVEFHECIFKA
                     TNNKKLIQLINSLWEQVYRFRVTYMSAYDTSHNIVEEHKYLLDAIIKGDSELSKKYAT
                     EHIEKAEQFMIDKAMISKDL"
     gene            complement(14720..15610)
                     /locus_tag="L3M60_000844"
     CDS             complement(14720..15610)
                     /locus_tag="L3M60_000844"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBN6225538.1"
                     /translation="MDFIRLKSRAKINLSIDVLGKRQDGYHFVEMIMQTIDLYDIVKI
                     KELEEDEIKVKSTSLDIPLDEDNIVYKAAKILKNKFYIKKGVEIFIEKNIPVAAGMAG
                     GSSNAAAVLVGLNQLWKLRLSEDELKEIGLNLGADVPFCISGRPALAQGIGEKLTSIK
                     GLPCDTNILICKPDLFVSTKEVYQGLDLNNIKKRPNNKYLIECLKSEDIKAVSESMVN
                     ILENVTISKHKEISDIKQIMMKNNALGSMMSGSGPTVFGLFKNKEDALIGKKELLKKY
                     KQVYVVNSSQKGVEICGEFN"
     gene            complement(15728..17278)
                     /locus_tag="L3M60_000845"
     CDS             complement(15728..17278)
                     /locus_tag="L3M60_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBN6225539.1"
                     /translation="MELIKDVIKVDNRIDFGKFQTFIEAEAVVPDKKLDVYEIVKTEG
                     YISLKKIEMADGKILCRGSFNYNVIYIADDKNTISNVDGKVDINEVIEKDNVVQDMEY
                     MLFSEVEHMDCTIMNERKIKVGALMNIRGSLFEKQRLDIVKDVAQVEGIQKHRKEICF
                     QDIVGIEKAESSIRDTITINTEEIQSIISLNPCVKVKESRVTDNKVIIGGVLEINPLA
                     CTYEGELVELDRVGIEFTQFVEVPGVSDGMTEEVLLSMSDFNHIFKQNSESNTGLLEI
                     DCMACCKVKVTDEVTREVLQDAYSPQKIIKFDHKPIQLNKTLRRSEETFMVREGIRND
                     NDDIQIKDIVSVCPTMSIENSYIEGNKSIIQGIIKVEILFVPVEGLKLVYKISEEIPF
                     EHDIEMDNLTDTASVFNTACIDKVEVDLNRDQIDLIIKIKRFTEALDKKAENFIIKGE
                     DQGVYDLSKAPSIIVYICKEGDTFWNIAKKYNTTENEIAELNDIKLDEPIKPGKCLIL
                     EKKVVLVD"
     gene            complement(17371..17637)
                     /locus_tag="L3M60_000846"
     CDS             complement(17371..17637)
                     /locus_tag="L3M60_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HBN6225540.1"
                     /translation="MATVQTLDKIRVSLERHIGKKILLKANKGRKQIITKEGILEKVY
                     PSVFVIKLDDESNGYPRVSYSYSDLLTSNVKLQVFRDQDKLQIS"
     gene            complement(17785..18645)
                     /gene="yabG"
                     /locus_tag="L3M60_000847"
     CDS             complement(17785..18645)
                     /gene="yabG"
                     /locus_tag="L3M60_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HBN6225541.1"
                     /translation="MKVGDIVARKSHNKDIVFKIVSFGVDENNEKIAILKGIAFRVIA
                     DAYIDDLELVKAPDIKDILIDKNVENLLYKSVRKAKERQKRMTRAVPKLQLNPNTYGM
                     PGKVLQIDGDKEYLKICLDVYAQLGIPAVGVAVSEQNQYKEVRALLEKYNPDILVITG
                     HDAMTIKRGDIEDMSNYRNSSNFVKTVKEARKWQPNLDNLVIFAGACQSNYEKIISAG
                     ANYASSPGRIMIHALDPVFIVEKIACSRIDVVVPIDEVIEQTITGVKGIGGSETRGKF
                     RWAMPKTILH"
     gene            complement(18729..19667)
                     /locus_tag="L3M60_000848"
     CDS             complement(18729..19667)
                     /locus_tag="L3M60_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HBN6225542.1"
                     /translation="MKFIDLHCDTIAKLMENVETSELKSNKYSVDIDRLKKGDSLAQT
                     FALFVDTEEVKHPFDYCMSMATKFHEEMKKNSDEIALATNYEEIMKNQSEGKLTALLS
                     IEEGAVLEGKLENLNKFYDLGVRMMTISWNHVNELSFPHNKLEYREKGLTDFGREVVH
                     KMNELGMLVDVSHISDGGFYEIAKISSKPIIATHSNSRAMMNHSRNLTDDMIKVLANK
                     GGVTGINFFHLFLSDKSESKLEDMVRHIKHIVNVGGIDVVSLGSDFDGIDSKVEIEDI
                     SQMGKLYEPLKKEGFSEDEIEKIYYKNALRVIKEVL"
     gene            complement(19777..19938)
                     /locus_tag="L3M60_000849"
     CDS             complement(19777..19938)
                     /locus_tag="L3M60_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225543.1"
                     /translation="MFILRFADDDDNLSVKDFKSLTDLKKYISENNIDKTWHQIEEVK
                     KVIPNLKEE"
     gene            complement(19948..20445)
                     /locus_tag="L3M60_000850"
     CDS             complement(19948..20445)
                     /locus_tag="L3M60_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C domain-containing protein"
                     /protein_id="HBN6225544.1"
                     /translation="MSLYTEWRNLSDNHENQEAEVKFWEEYLKVEAGIYNEILNNKMD
                     VVEGKVSELAEKFGTTNEFFMGFIDGISESLKEDIVLEDVEADKEVSIKIDWEKLYNN
                     MVTVEAHWLYNLQGWEDILPADQRKEIIKAYKKSKTIVKEAKIGRNDSCPCGSGKKYK
                     KCCGK"
     gene            complement(20560..21270)
                     /locus_tag="L3M60_000851"
     CDS             complement(20560..21270)
                     /locus_tag="L3M60_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0489 family protein"
                     /protein_id="HBN6225545.1"
                     /translation="MENYEYSGFYIEKPVGNNVFSYDKRENKSIYVPKLINGTLNNVR
                     LGNEVVFNEVDENKEIKAKGLENMVEYVLGNKKIYVFDNHNHAFYFWIKSLLKGEFTK
                     GCKLVHVDQHKDTREPENYNVDINNIKDVFRYTNEVLNVGSFIKPALKYNIFSELIII
                     DSLYGFGLDVESEFVLDIDLDIFSSDMDYIPFELKFYKIKNLIKKAKVITIATSPYFI
                     DQEYAIKVLKELFNYDII"
     gene            complement(21443..21973)
                     /locus_tag="L3M60_000852"
     CDS             complement(21443..21973)
                     /locus_tag="L3M60_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225546.1"
                     /translation="MDIRALPTKITKEEAIDIAKSGKNSLFSKLILKNKYAHEVRLHY
                     IEFKIVTLKIHKTKSIFKSRRYSNGVNELKILVNGSTGSGSIVQDFPTYEIIKNVDCN
                     IVQYSDKDERRVKAKANKIAMRLTHRFMGGIPEIEIVKIESIFRPYWVVFFGKVELNR
                     RVSYLPIEADGFVIGM"
     gene            complement(21990..22220)
                     /locus_tag="L3M60_000853"
     CDS             complement(21990..22220)
                     /locus_tag="L3M60_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225547.1"
                     /translation="MSKLVSKVKKDLEIKWLGIDGFLVLLYFLITGFIFYGILTRNDI
                     IVGRIIPYGVMAVVIIIFFDYIYRVSKMTRKK"
     gene            complement(22235..23629)
                     /locus_tag="L3M60_000854"
     CDS             complement(22235..23629)
                     /locus_tag="L3M60_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBN6225548.1"
                     /translation="MLTFWENGIIVIFAFILLGIGYYFSRNVKDMESYYLGNRSLPWS
                     LIVGTLVASWYGGVGTVGSVEYAAIYGISAWAVWSIAAHIGRMPLALWVGPRIHIRTN
                     ITVPDLLESFYGKHVAIIGAILMFIYCAQLGEITAMGTIGNVAWRIDKELIGLIMVGI
                     VVVLTVLGGLMGVAITDMVLFFCMVFGLTMVMPGQFEQIGGFQGLTEALKDAPQLMHP
                     TKGMSAMKALMLIVYSFGAYSDPTFYQRFSAADSPKVGRRALLTCFSLWICFDMVLTM
                     TGLIVRATYPEMAPAAGYITLVLDTLPQGIRVIFIIGILGSIISTLDSYYLAAGATLA
                     NDIYGRITKKELSQKQLVKFTRIGVCLAAVMGLSVAFRFENVYDACIFVGSIWMGSAF
                     VPIVGGLLGNGRRTSMGGILGMIVGGVTFGYFKIFPVQSFELEPLVIAIPFSFIAWMI
                     GNRIGENLNTQNNL"
     gene            complement(23755..25008)
                     /locus_tag="L3M60_000855"
     CDS             complement(23755..25008)
                     /locus_tag="L3M60_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225549.1"
                     /translation="MENKSNVKRFLIIFILAFGTTAMYSLPYMKSSFYDPMQQALSLS
                     HTQIGNLLSLYGLVGMVSYFIGGWFADRFSVRKLITFSLIASGALGFYFSTFPSYSMI
                     LLIFVLWGFTTILTFFSASVKVVRMQGSESEQGRIFGFYEGLSGVSGTLISFIGLYFF
                     GKFAEITVGFKYVVWLYSAASIICGLLLFFLVEEKKDSGASDEGLSIKSLLKVVTMPK
                     AWLIGLIIFSTYLVFSSLTYLSPYLSEVYVMPMTLVSALSIIRTYVIKMGASPVAGVI
                     TDKVGSSIRVMFVGFILMTVSTAAYLVIPKSTGFIWIAVINMIILSVILFGFRGIYFA
                     SVSESNISLETTGAVVGFASFIGFSPDAFYYTIAGNWLDKYGQTGYTYIFILSVVCAV
                     IGIFATYALNKINKRENKSLNNKIA"
     gene            complement(25059..26213)
                     /locus_tag="L3M60_000856"
     CDS             complement(25059..26213)
                     /locus_tag="L3M60_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6225550.1"
                     /translation="MSNNINQDLETKIIHWGHSADPTTGALATPICQTATFAAKTVEH
                     FEELCMTWGYVYTRECNPTLTELEAKLAMLENAESAISSTSGMGAITSTILALVKSGD
                     HIVSSDGIFSHTKLFMSELLSKFGVEVTFVDAVNPQNVKDAMKPNTKIVYIESPLNPS
                     LDLVDIKTIAEIAHENKSLAIVDSTFGTPIVQRPIDLGADLVIHSLTKFINGHGDTLG
                     GAVAGSKELIDLVRWPSLCCFTGASLPPMSAWMILRGMKTLDMRMKKHCENGLAVAEF
                     LEEEEKVELVKYPALKSHPQYELCKTQMNGLGGGVVSFKLKDGINGLTRDQASRRLMN
                     SLELATIATSLGEEHTLVQMNGENLIRIAVGLESSNDIINDFKQAIEKLK"
     gene            complement(26774..27346)
                     /locus_tag="L3M60_000857"
     CDS             complement(26774..27346)
                     /locus_tag="L3M60_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN6225551.1"
                     /translation="MIIIYGYCRVSTKNQTDNNSLYQQQQEILNKYNDAKIIKETYTG
                     TTTNRPKFEKIISSLKKGDLLVATKLDRLARSTSEGIQLIEGLFAKEVSVHILNIGLL
                     ENTSMGKFFLTTLLAVAELERNMIIERTQLGKEFARKKKDFKDGRPKKYSNQQIQYAL
                     GLLNSKPYREVVKMTGISKSTLIRAKKSIQ"
     gene            27549..27776
                     /locus_tag="L3M60_000858"
     CDS             27549..27776
                     /locus_tag="L3M60_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN6225552.1"
                     /translation="MNSLLKEAIDELKNIHCGEVFLLKELFKGYEWNRLEMNIRLKLG
                     KLFLYEVVTNPNLNVEVINKNSAYQQMYKKK"
     gene            complement(27996..28739)
                     /locus_tag="L3M60_000859"
     CDS             complement(27996..28739)
                     /locus_tag="L3M60_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBN6225553.1"
                     /translation="MENYQSTNTCTYNILYVQFNEDFERSKYFSYDVKYPVIYNIRNQ
                     STYVNPVILNNINNAVRMTVNNFKNGLQEEEQEYNNAASQNSLPKQSYWVSTSYAVTF
                     NKNYVLSMILSLMGFANNFGPKYNTLNNYNIDLTTGKELLLNDIFRDGVDYINIITDY
                     IKSQINENREMYYSNVELVIPEDQAFYLTDSGIVIYFGVDEIAPESFGIPKFKLKFSD
                     FGNFINSRFYCISPDIYVQSRGRVKYFMG"
     gene            28906..29778
                     /locus_tag="L3M60_000860"
     CDS             28906..29778
                     /locus_tag="L3M60_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN6225554.1"
                     /translation="MLLLADKWKDYELIDMGNGEKLERWGNYVLRRPDPQVVWPMESE
                     WALWKNPHGHYHRSNKGGGQWEHKKRYPEQWTINYKNLKFHIKPTGFKHTGLFPEQAA
                     NWDWMIEKIKKANRPIKVLNLFAYTGGATVACASAGAEVCHVDAAKGMVNWAKENIEL
                     SGLKNQTVRFIVDDVVKFVEREIRRGKKYDAIIMDPPSYGRGPKGEVWQIEEKLYSFV
                     DLCMGVLSDNPLFFLINSYTTGFSPIVLENVLSTTIGKKTNTGYIYGGELGIPASRDG
                     KILPCGIFGRWEKK"
     gene            29778..30443
                     /locus_tag="L3M60_000861"
     CDS             29778..30443
                     /locus_tag="L3M60_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBN6225555.1"
                     /translation="MIKVIYEDNHLLVVEKPVNILSQGDNTNDKDMVNLLKDYIKKKY
                     NKPGNVYIGLIHRLDRPVGGVMVFAKTSKAASRLSEQVRSKTFKKTYLAVVHGKIKSN
                     SDILKDFLYKNKKTNMVTVVKKNHKDAKEAELNYNVLNFKENLSLVQVNLKTGRSHQI
                     RVQFASRKHPLYGDQRYGKEINKIGQQISLWSNKIEIYHPTTKEKMEFTCHPPDKYPW
                     NIF"
     gene            30464..31321
                     /locus_tag="L3M60_000862"
     CDS             30464..31321
                     /locus_tag="L3M60_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6225556.1"
                     /translation="MSKKFSIGEMSKLHKISIQTLRYYDQIGLFKPVHVDQESNYRYY
                     SIDQFSQLDIIKYLKYLGMPLKEIKSKLDENNEEILKLLTNKINIVDEKIKELELIKK
                     VLSAKKETMEKGMKVDEVGKIVRKHIPRRSILSIEYEEGLSSEETMELSRRQIANILE
                     DNISVFYGGVSGLVSIDKFLKEDKVIYDNSFVTVERDLFDIKSQKYITEISAGDFICM
                     TYKGPYKENYTAFKKLVDYVEYKRIPIENYIYEIPIIDPLSTNNEEELLTEIQIQVKK
                     REIDLRCLV"
     gene            complement(31458..34004)
                     /locus_tag="L3M60_000863"
     CDS             complement(31458..34004)
                     /locus_tag="L3M60_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225557.1"
                     /translation="MNIFNKVALQGLKKNRTRTFVTIIGVVLSAAMITAVTTFGVSLM
                     NYMINGAIIKYGDWHVEFLDVDSSFVQEQRVNDEVENTVTFENIGYATLEGSRNPDKP
                     YLFIAGFSKEAFDTLPITLISGRLPENSREILVSGKVATDGGVKFAVGDTISLDVGKR
                     MSENENLGQSDPYTAGSEAFIPQNKRTYTVVGICRKPVFEESSAPGYTLITKEDEQVK
                     ADSLSLFVTLKNPRHVRSYTSSAREHDYIFNDNVLRFMGISDNNLFNMFLYSVGGVVL
                     AIIMLGSIFLIYNSFNMSLNERTHQFGILSSVGATAKQLRNSVLFEGICIGVIGIPIG
                     VVIGIVSIGLVISVIAKNFANILYVNVPLTLTLSIPAIVVAAVVSMVTILISAYIPAR
                     KAANTPVMECIRQTNEVKIESKSVKTSKLTQQIYGLEGVLAMKNFKRNKKRYRSIVLS
                     LVLSIVLFISTSALVKDLKQMSNQRSEVTDQDIIFSTQGMKDSEMLKLYDKLKTASGV
                     YEGLYQKLMTYSCVAKVSELTDDYLNYNGLHSMSEMLELPVDMQFLDDRTYLNIIKSL
                     GLPTEEYTGQNAKMIAVSMLTKDNHIIDIFTNSSMDVSLTPEVNGKLDTPERQNVNIS
                     FVKVVPPDSPPGIRNSDMKKASFRVLAPYSAKDKFEIPGVSTGIESLSFQSKNPTQSV
                     SEMEQIIESEGVTNEYNLYNMYGMMDENRNYIFIANVFAYTFIIMISLISIANVFNTI
                     STNIKLRRRELAMLRSVGMSEHDFQKMMNFECAFYGMRALFFGLPIAVISSWLIHKVM
                     VTDDIDFVIPWVSIGISIFSVLFIVFITMLYTINKIKKENIIDALRDDMA"
     gene            complement(34004..34690)
                     /locus_tag="L3M60_000864"
     CDS             complement(34004..34690)
                     /locus_tag="L3M60_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225558.1"
                     /translation="MAFLKIENLCKVYGKNENQVTALDHVSLTIEKGEFTAIIGSSGS
                     GKSTLLHSIAGVDVPTSGKIYLEGQDVYGQSNEKLAIFRRRQVGLIYQFHNLIPTLNV
                     VENITLPILMDKRKVNQERLNDLLELLGLKERKTHLPNQLSGGQQQRVAIGRALMNAP
                     AVMLADEPTGSLDSKNGQEIIQLLKESHSKYHQTLIIVTHDENIALQADRIICISDGK
                     VVRDERKVNR"
     gene            complement(34773..35762)
                     /locus_tag="L3M60_000865"
     CDS             complement(34773..35762)
                     /locus_tag="L3M60_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225559.1"
                     /translation="MFRNREFRRFAILFLLITILTVALGFAISTMTGIISIVSATTFG
                     TAFFVFTKNRYKSIAQISEQINLVLHNANHLYITESDEGELSILQSEITKMTLRIREQ
                     NYALKKEKEHLADSLADIAHQLRTPLTSVTLILSLLENTSDEDERKELIRETEELLIQ
                     MDWLITSLLKLSRLDAGIVVFQKEEIDVDNLISSALHQLLIPMELHNITLHINIPKGV
                     KILGDLNWLSEAIQNIFKNCMESVGDNGKIDIVCEDNFLFTQLTIHDNGEGFKKEDLP
                     CLFNRFYRGKNSSTAGYGIGLALCKTIIMRQGGTITAENHPQGGAIFVIRFPK"
     gene            complement(35749..36435)
                     /locus_tag="L3M60_000866"
     CDS             complement(35749..36435)
                     /locus_tag="L3M60_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225560.1"
                     /translation="MKRIFLVEDDKAIAKNLILLLNSEGFIVTHVSTRSEALVEIAKN
                     KFDLALIDISLPDGNGFTICTEIKENQDIPVIFLTASGDESSVVTGLNMGADDYITKP
                     FRPRELIARIRTALRKSGRFGSTFEICGLYVDTATGVVKKNGNEVFLSALEYRLLLVF
                     ISNPKSIITRVRLLDELWDAAGEFVNDNTLTVYIKRLREKIEDDPSSPQIILTVRGTG
                     YRLGGSYVSK"
     gene            36853..37182
                     /locus_tag="L3M60_000867"
     CDS             36853..37182
                     /locus_tag="L3M60_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6225561.1"
                     /translation="MDLNKEVLKGHIDTLILSILNKGDSYGYEIAKIVINETTFELKE
                     STLYVSLKRLESKGLIKSYWGSDQGLGSRRKYYNITDEGKENLELKIQEWNFIQSVMN
                     KFLKRGE"
     gene            37187..37636
                     /locus_tag="L3M60_000868"
     CDS             37187..37636
                     /locus_tag="L3M60_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225562.1"
                     /translation="MKIIEEYLEALYKNDTSKEIQDLKEELKEHLIDSTNEFIENGYD
                     INEAQNKAIERFDGSNGVSVELRSIFKQKIEPKKVLLRKLSNSRIVFLNLFGWLLAAT
                     AYTINRGFGHIAPPWIIILLIISIVILIILTIIIKGLKKEIENDYNI"
     gene            37703..38080
                     /locus_tag="L3M60_000869"
     CDS             37703..38080
                     /locus_tag="L3M60_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBN6225563.1"
                     /translation="MVKKILLEKFLDTSGVSYINEKLNLFKDTKWISFGTYIMTVDSF
                     CYREDSKHYNPIMSTFDYESEIFIVNALYKENIYSIKSKKLYRNVSKGDKIIVTIKYS
                     YDKYENLIHEEIHDYHLYPLSLD"
     gene            complement(38265..>38651)
                     /locus_tag="L3M60_000870"
     CDS             complement(38265..>38651)
                     /locus_tag="L3M60_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBN6225564.1"
                     /translation="VVLATVNNHDKDEFIEIAKDMKELGYTFVATEGTAKSLRENGIE
                     ADIVNRIEEPRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKMRRTAIEFSTEIMTSL
                     DTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT        12508 a         7076 c         4346 g        14721 t
ORIGIN      
        1 atttcgtcaa tacttttttt gttttttatt ttaaaaaggt tataactttt tgtttttctt
       61 aataatttta attaaaacca atagatataa cccttgatat tacaaacaat acatgcatat
      121 ataattttac ctattttgca attttatttt cataagaata tgaattagct ctgactctat
      181 cagcccaaac atttatactc ttaactttat taacattagt tgtgttctca aaaatatcca
      241 ttgcatcctt acaaaaatat atcataacat caagaactaa aaatccaaca cagaataaac
      301 ataaaaaatt aatcacttga aacatcagac attcccccaa taatttttta tatatagtat
      361 tctcagaaat tattcttttt accttctcaa aaattattct aaatattttc ataataaatt
      421 actttcaatc atattttcta aatataaagt ctcaatcaac aaaaactaat atcaatttct
      481 aacgtttgtt ggtaatttat aaattaaata tctaaaagaa tctttgaaac atataaaaag
      541 cattctacac tgacatttta aataaagttt agctatttat tgctaattta atagctaaac
      601 tttatttaaa acttagtaga atgctaatat ttcttagttc tcttcttctt tatttggatt
      661 gttatcaata ggtttcatag tagggaataa taatacatct cttatagatg gagagttagt
      721 taataacatg attactctat ctatacctat tcctagtcct cctgtaggag gtagacctac
      781 ctctaatgct tttaagaaat cttcatccat ttcaaaagct tcatcatcac ctaattctct
      841 ttttctcaat tggtctataa atcttccttt ttggtcaata gggtcattaa gttcagaaaa
      901 tgcattagct aactcccact tattagcaaa tacctcaaat ctatcagttc tccttgggtc
      961 ttcagcattt ctcttagata atggagatac ttcaactgga tgatgagtta taaatgttgg
     1021 ttggattaat ttatcttctc caaattcttc aaagaaagca tttattactt ctcctcttct
     1081 cattccaggt tttatctcta tacctttttc tctagcaact tctaatgcct cttcatctgt
     1141 atttatagta gaaaaatcta caccagaata ttctttaaca caatcttcca tactcattct
     1201 tttccaaggt ggtgtaaagt ttatttctgt tccttgatag ttaactatca tacttcctgt
     1261 agcaacttca gccatatgcg caataacatt ttctgttatt tccatcatat cagtatagtc
     1321 tgcatatgct tgatataatt ctattgcagt atattcagga ttatgtttta aatccatacc
     1381 ttcatttcta aacattcttc ccatttcata aactttatca aatccaccaa caataagtct
     1441 ttttaaatac aattcatttg ctattcttaa atacattggt atatgtaaag tattatgatt
     1501 agttataaat ggtctagcgt ttgctccacc tgctatagta tttaatattg gagtctcaac
     1561 ttctaaaaat cctctatcat ctaagtatgc tcttaatgct tttaatgctt ttgttctaat
     1621 taaaaaagca ttttttactt ctggatttac tattaagtca acatatcttt gtctatatct
     1681 tagttcttgg tcttttaatc catgatattt ttctggtaga acttgtaaag atttacatag
     1741 taatactacg gatttagcct ttacagagat ttcacctttt ttagttttaa agatttcccc
     1801 ttcaattcca actatatctc caatatcata tgttgaaaat acactatatt cttcctctcc
     1861 tatagcatct agtcttacat aagattgaat tcttccctct tggtcttgaa tatctataaa
     1921 gccagctttc ccttgtattc tcttgctcat tatacgtcct gctatttttg tagttttgcc
     1981 ttcaagagat tcaaaattat cttttatctc catagaataa tgagttctat catatctact
     2041 ttctttaaaa ggatctctac ctgactcttg taattttttt agtttatctc ttcttacttg
     2101 aagtacttca ctaaggtctt cttgaatttg cgcttcttca ttgctttgtt gattattttt
     2161 catttttttc ctccaaatta tcttcttatt tctaatattt caaacttagc aactccatca
     2221 ggaacttgca cttccacaac ttctttcgca gctctaccta aaagtgcttt tcctacagga
     2281 gattcgtttg atattttacc atcatatggg tctgcttctg tcgaaccaac tatagtatat
     2341 tcaacctctt catcaaattc taaatcctta actttaacta ttgatcctat tgtaacaaca
     2401 ttaaggtcta ttttgctttc atctatgata acagcttttc taaccatatt ttctaatttt
     2461 aaaattcttt cttcaacttg tgcttgttcc tttttagctt catcatactc agcattttca
     2521 gataaatcac caaaagatat agcaactttt attctctctg caacttcttt tctctttaca
     2581 acctttaagt tttctaattc ttcttctaat ttatcatatc cctcttgtgt caataaaaac
     2641 tctttatttt cttccattat aaatacaact ccttcacaaa ttccttagga attttcctaa
     2701 ttatatttta aaaattttat aatctttttt taatttatgc gctatattat aagtcactat
     2761 tatttttatg tcaaggagtc ttccatatag tactccaatt tcgacacaac ttcttgatag
     2821 tcttctattt tgttaatttc atcccttaat tttgcagagt ttctaagtcc ttttaaatac
     2881 caagcgatgt gttttctcat ttcccttaca gctacatact ctccatgttc ttctactgca
     2941 agttttaaat gcttaatggc agtacttatt ttttcgttaa gagttggctc tggcaaaatc
     3001 tcgcctgtat tcatataatg gtttattctt ttaaatatcc aagggttacc ttgagcaccc
     3061 ctacctatca ttatagcatc acaaccagtt ttgtctaaca tatttattga atcttctatt
     3121 gtaaatacat caccatttcc tataacaggt atactcaaat ttttcttaat ctctgcaatt
     3181 atatcccaat ctgctttacc tgtataaaac tgttctctag ttcttccatg tattgctaga
     3241 gcacttattc cacaatcttc tgctatcttt gctatttcaa cagcatttac actattgtca
     3301 tcccagcctt ttcgtatctt taaagtcact ggcttttttg agttcttcac tactgccctc
     3361 aatacctctt ctgcaagctt aggatttttc ataagtgctg aaccatctcc atttttaact
     3421 acttttggag ctggacatcc catatttata tcgagaatct catttgaata atcattcatt
     3481 atctctgctg ctcttcccat aaactctggc tctgaaccga atatctgaac tgctacagga
     3541 tgttcttcat cttctatctt aagcattttt ttagtatttt catcatcata gcaaagtgcc
     3601 ttaccattta tcatttctgt atataaaagc ccacatccct gttctttaca aattaatcta
     3661 aatggaaggt ctgttacacc tgccataggt gatagaaaaa ctttattttt caattctaaa
     3721 tttcctattt tcattgtttt tctcctattt tttcctttat tttgtaattt aactttaaat
     3781 tcttatttcc tcattataat ataccacaca tatgtagaaa tctatcataa aaagggaatt
     3841 ggatatataa cccaattccc tgtatattaa ttattcttgg tttttttcat atattattct
     3901 caatccttct agtgttaaaa aaccatctac ataatctata cttttcgtct ctgaagcaat
     3961 cagtttagct aatccacctg tagctataac cttaacatca tcacaattca attctttctt
     4021 catcatattt attattttat caactaaacc aacatatcca taaattattc cagattgcat
     4081 agctgataca gtactcttac aaatagtcat acctggctta gctaattcta ctctaggtag
     4141 tttagacgca ctttgaaata aagcctcact agatattttt attcctggtg ctattgttcc
     4201 acctaaatat tcaccttttt cagagatagc acaaaatgta gttgctgttc caaaatcaac
     4261 aagtatactt ggtgctccat acttttctat acctgctaca gcattaacta ttctgtctgc
     4321 cccaacttgt tttggattat catattttat atttagacct gtttttatgc ctggacctac
     4381 tattaatggc tgttttttac agtactttat acaaaagttt tcaagagaat gcataacatt
     4441 tggaactaca gatgatatta taacatcatc aatatcactt atattcacat tatcataatc
     4501 aaatagatta cttatcaaga ttccatattc atcagaagtt ttttctctat ctgttttaat
     4561 tctccagtaa ttaactaatt tgtcaccttt atatatacct aaaaccatat tagtatttcc
     4621 aacatcaaat actagaagca tatgtcatta accttctttc tattttttgc ttttcttaag
     4681 tacagtggca acagcagcta ctactaaagc ccctactatt gcttctggca ctccatttgc
     4741 tgcggcaaga gccattatta cttttcctgc tgaaccactt ttacccattg catgaagata
     4801 cttgtcagca tataagacat atatcattcc taaaactcca gctgtattta acagagaacc
     4861 tatagcccct gtaattaaac ctgatatgta taccttgttt gtagccttaa ttattaactg
     4921 atatacataa taagccaata cacctatcat aactcttggt aatattgaaa ctaatggatt
     4981 tataaaaaca aaagatgtca ctgttgggtt tattatagca tttaagagac ttgatatccc
     5041 aaatataaat cctattgctc ccccaactat tggtccttcc ataatagctc caataataac
     5101 tggtatgtgc attatagttg cttttgtagg tccaactgga ataaatccca atggagtcat
     5161 agatagcata atagaaatag ctgatagtac tcctattata gtcatctttc ttacatttac
     5221 ttttcttact gatgcacttg tgttcatcat aaaaaataca cctccgttcc agctcattac
     5281 gatgccggac tacttaattt tttaaatttt tttcgttttt ttcttataat tatgagtcaa
     5341 gctaccattg taggatgccc gctctaacaa gaataatttt atcaagatga gttgccattg
     5401 tcaacaattt gacaagaaaa atatgctaat ttcatcatat ttctcatttt tttactatat
     5461 ctttatatgc cattatattt tctacactat ccgctttttt tactgcattt attatgcttt
     5521 tttccacaac ctctacagcc aatgaaccta ctaatgttat atctgcttct atttctcctg
     5581 ttgatattgt gaatattgta tccccatcat gaggtgtatg gataggaaaa atagctttag
     5641 catatccatc atgtcccatt tgagatatct ttttacatcc agctttagtt aacttagcat
     5701 ttgttgcaat tatcccaata gtagtattat ctacactaaa tccaccttta ctttcacctt
     5761 gtttcattaa tttatatgta tcaattgttt ctgtcttttt atcatttaaa gcacccgcta
     5821 taaccttacc attttcataa acatctccaa gggcatttac agcaattaat gcagatacga
     5881 ctagaccatt atctaatttt atagaataac tacctatgcc acctttcata gcatattctt
     5941 gacctcttat ctttccaaca gtcgctccac aaccagctcc ataatttcct tgatttaaaa
     6001 tgtcaatatt tgcaacttca catgctctat aacccatctt taaatctggt ctacatttaa
     6061 aattacctac tcctaaatca aatagaactg ctcctacaac tattggtact ttacctacac
     6121 ctacatcaaa gccaattcct ttttcttcta agtatctact tacaccacaa gtagattcaa
     6181 gaccatatgc agaacctcca gccaaaacta ctgcatgaac tttttgaatc atatttacag
     6241 ggtctaataa atcggtttct ctagtccctg gagcagaacc tcttacatca acaccacaag
     6301 tagctccttc ttcacatatt actactgtac acccagtaag accatttcta tcctcaactt
     6361 gcccaacctt taaaccttct atatccaaaa tattattata catatccctt ttctcctctt
     6421 atagaaactt cacctgaaat aatttctttt atatttccat cacttctttg aactaataaa
     6481 tttccctctt catttatatc taaacatttc actagttctt tattatctcc ttttaataca
     6541 tatatatttt tgtctataat tgcagaataa tctctacata tttttaaggt tttttctttg
     6601 gaattattta cagtataatc taaatataat ttttcaaatt cagtcaatac attcctaaca
     6661 atatctactc tggataacat gtatccttct ttatacaatg atgtagcttt ttcagctatt
     6721 tcttgttcaa aatccattat tttaacattt attccaattc caagaacaac gtagtttgtt
     6781 ctttcaattt cagcagataa ctctgttaat ataccacata ttttttttcc atttaaaata
     6841 acatcatttg gccattttat taatgtattt gctcccatgt tatttaaagc cttcgttaca
     6901 cttgctcctg ctattaaagt tataaatgga gctttgaatg gttccatatc tggttttaat
     6961 ataatactca tccagattcc ttcatgaggt tttgaatccc atcttcttcc caatcgtccc
     7021 ttccccatat cttgatgttc gcttaaaatt acagttccat ctaaactatt tacagctatc
     7081 cttttagcat gttcatttgt tgaatctata gtttcaagat gttcaatatt ttttccaata
     7141 aactctgtat ctaatttata gcaaatattc tgtgggctta ataaatcagt tggattttcc
     7201 gctaatcgat accctttttt atttacagat tctatattat atccctcttc ctttaaggct
     7261 ttcatatgtt tccatatagc tgcacgtgac acccctaatt gttttgataa ttcttctcct
     7321 gaaataaatt cagatttatt atttaacata atctctacta ttttatctcg catatttcac
     7381 ctctaatatg acatgatttc atgacttatt ttcttctaat aaataattat acattattaa
     7441 tatactttta atactattaa ttttaaattt ccttttcatt tattataata gttgttttat
     7501 attttatatt gatgatttat gtgattttat attttattta aataatttta aaataataaa
     7561 aaactgaaag tatcaatcaa attaatacat gcaaatatac tttgcttatt aatttaatat
     7621 tatactttca gttttagtat ttaatccata tcagataata atcttaaaat gaccaaagtc
     7681 ttaaaactat ttatctttat cagatttatc ttctaaattt ttatttatat caataacatt
     7741 gtttctttct tcttcatctt tttctaattt tatagttaat tgctcttcaa cttctttaga
     7801 agaattttcc tctgtaactg cattttcaat ttcattcaga ggtaactctt tattaaatgc
     7861 ttttacaaat tgttctgcat ctagagtttc atatataagt aaagcttgtg ccacatattc
     7921 taatctatcc atattttctt gtaatatact tcttgtctta tcatatgcac catcaactat
     7981 acgttttgtt tcctggtcta tctcaaaagc aacttcttca gaataatttc tcttacttga
     8041 aaaactatta cctaggaata cttcatcatc actgtcaaat gacattggac caagtttaga
     8101 actcattcca tactttgtta ccatacttct tgcagtagct gtaactcttt ctaagtcatt
     8161 tgaagcacct gtagatacat ccttaagtac tagctcttct gctactctac caccaagtaa
     8221 aactacaata ttttctttca tttcattttt tgtagcatag aacttatctt caacaggaag
     8281 ttgcattgta aaccctccag ctcttcctct tggaactatt gtaacttgat gaacaggact
     8341 tacttcttca agtacatggg cacaaactgc atgccctcct tcatggtatg ctgtaagtct
     8401 tctttctttc tcacttatta ctttagattt tttagccaca cctgctataa cctttgttat
     8461 agcttcttct atagtttcca tcttaatttt cttttctctt tttctagcgg ttaatattgc
     8521 agcttcattc attaaattct ctatatcagc aggagtaaat cctggtgttc ttctagccaa
     8581 gacatccatt tttacatcat cacttaatgg cttatttctt gagtgaactt taaatatagc
     8641 ttctctacct tttacatctg gtgtaccaac aacaacttgt ctgtcaaatc ttcctggtct
     8701 aagtaacgct gggtcaagta tatctggtct atttgtagct gccataatta ttataccttg
     8761 atttactcca aatccatcca tttcaactag gagttgatta agagtttgct ctctttcatc
     8821 atgacctcca ccaagacctg cacctctttt tctaccaact gcatcaattt catctatgaa
     8881 tatgatagct ggagcacttt tcttagcctg ttcaaataaa tctcttactc ttgaagcacc
     8941 aaccccaaca aacatctcaa cgaaatcaga accacttata ctgaaaaaag gaactcctgc
     9001 ttctcctgca actgctcttg atagatatgt tttacctgtt ccaggaggcc ctaccataag
     9061 cattcctttt ggtattcttg cacccagttc aatatatttc ttaggattct ttaagaaatc
     9121 tactacctct tgtaaatctt ctttttcctc atctagtcca gctacatctt taaatgtaac
     9181 tctagttttt tcatcatctt tatgaacctt agcttttgat ttaccaaagc tcataacctt
     9241 tccgcctcca ccttgagact gattcataaa cacgaaccaa ataatcacca taaagaatat
     9301 taacagtaat gtaggcaaca tttcgacaaa ccaaggtgta gatggcttag cttcaccacc
     9361 aaaagttagt ttcccagcct tagcttggtc taacacctca tctgcaagtt tatttcccat
     9421 cacttctgtt ggaacatacg acttaaactt tgtatttgta tcttttatag taccttctac
     9481 agaagtttga tttacaaagt ataatcttga tatattctca tctgttaatt ctctatatac
     9541 ttcagagaat tttaaatctt ttattttttc tgtaggctta cctgagaatt gaactatacc
     9601 tactataatt ataaaaacaa gtagataaaa acctgcccct tttagcaact tattcaatat
     9661 agaagccctc ctttcataca ttgctttgcc ctataatttg ttaaaattaa tttttaatac
     9721 ttcttttgtg ttttcatcaa ttttataatt ttcactgatt ttataatctc caacacatat
     9781 tattccttca ctatctgtga ttataggaat tttacatctt tcttctcttg ggatttttaa
     9841 atctatgaat aaatctttca cttttttact tcccatagca agctttatct tatcaccttg
     9901 ccttctactc cttattacta tacccccttt taccttatta aaatcaaacc ctttagatga
     9961 attgtctaat ttcatactct tatacctatc tatactcatt acttgggtct caataatttg
    10021 ttttaattct tttattttaa taaacccatt actaggtata ttgtaataaa attcaatttc
    10081 ctcactaaca atttcctcat tcgttaatat tatactgtct ttttttctat aaacaaatat
    10141 atttcttgga agagttaaca ttttatttac ttttgaatta tcttctaaag acattatatc
    10201 ctctatatgt ctttggtcaa caaaattagt atctcctagt atatgtttta tagaatttct
    10261 aagaacccta actttgatag catcatgtaa gcaaacaaaa tcgtctaaat ttatctctac
    10321 aaagcctttt tcttttatat ttgaaacttc tctaaattta gcttctgcct ctttctcaat
    10381 ataatcgtta tcacttttta aactattgct catcctcact atagattcta ttacatttga
    10441 attaaaatta tccttcatat atggtaaaag gtctaacctt atcttatttc tagtgtatat
    10501 attttctaaa tttgttttat ctatcctcgg atttaaatta taagcttcac aatactcctc
    10561 tatctcactt ctttccacat ctaatattgg tctaattatg caattatctc taatatagtc
    10621 aattcccttt aaacctttta aacctgttcc tctcatgata cgcatcataa cagtttcggc
    10681 ttggtcattg agattatgac ctattgctat tttattagct ttaatcttat ctttgatttc
    10741 ataaaacatt tcatatctta gcttccttgc tccttcttct aatgataatc cctcattttc
    10801 acaatatttt ggtacattaa tagactttac aaagaatata attcccatat cctcacaaag
    10861 tttagataca tataatgcat ctttttgagc ttctatccct cttatttgat ggtttaaatg
    10921 tgctgcatat atttctatat taaaatcttt ttttaatcta tttagtacat gcaataaaca
    10981 aactgaatca ggccctccag aaagacctaa aactatttta tcgccttttt gtattaaatt
    11041 atgtttattt atagtactta gtaccttatc aaatatcata tttatccctc ttacacttag
    11101 ttttattcac ttttaagtat actttctata aaaaaacgct aaacccttgt atcaatttta
    11161 agaaattaaa gtttaatata aatataataa taccaaataa attaaataaa aggtagtttt
    11221 ttcactacct ttataaaatt tctggaaaat agctatagtt attatttagt atctatgtat
    11281 atatctattt tattcctacc taaaaataaa tcttgtacat ttatattata gtctattttc
    11341 aattttatgt gttccccttc ttgtatattt atatccaatt ttcttgtatc aatgtcaatt
    11401 aataaagtac cttgtggggt tacatacttt gtggtactac tctctccaca tttaaacagc
    11461 attgttgagt taatagcacc aaattttttg atactaactt catttttaag tatctttata
    11521 gtattagtta tttctatatt ttcttccttt tccttgtaaa caacatatat tccatcattt
    11581 ttataaaaaa ttttaccaaa ggcagtcatc tctatggtgt ccatatttcc tttttcatca
    11641 tattgtctag tatttatact tatttttaca ctcaacgatt tttcctccat agattcttca
    11701 tataaactag tatttttgta tttctacttt ttgaatccta tcaatttttt tctttaagaa
    11761 ctctcttgct atatctgtaa atttttcagg tatatctgat acataatatt gataagttcc
    11821 atgaatttct gtattgttta taatattttt cacctctaaa acctttttta aatcctttgc
    11881 agtctctttg gctggattaa caagttttat atgctctcca acttcttcgc ctattgttct
    11941 ctttaaaata ggatagtgag tacatcctaa cactaaagaa tctatatttt tttctttcaa
    12001 ttcttgtaaa tatatctttg ctgtcaattt agctacttct gtatttgccc atccttcttc
    12061 gacaataggt acaaataaag ggcatgcttt attaacaatt tctatactct catctatttt
    12121 agatatttcc aaattgtatg cttttgaact tatagttcct tctgttccta ttattcctac
    12181 aattttgttt tttgtagaac ttacagctgt tctagctcct gcttctataa caccaattat
    12241 cggtatatca tacttttctt gagcttcttt taaacttctt gcagtggcag tattacatgc
    12301 tataactata gcttttacac cttttgtttt taaaaagttt atagcttgaa aggtatattt
    12361 aattatagtt tccttagatc ttgaaccata tggtattcta gcagtatctc caaaatatat
    12421 tatatcctca tttggtaata tcttcattat ctcttttaaa actgttaatc cacctaatcc
    12481 cgaatcaaaa actcctattg gtttattgct catctttata caacacctac tttacatttt
    12541 tgtgtttaac aacaacttat ttatcactaa aatatttatt tatattcata attaatatat
    12601 tattacctaa atgattatta tagtttattt tacacaaaaa ataaaggagc tatcttaaat
    12661 tagaaaatat tttctagaaa aagacaactc ctttcactac ttaaaacttt aaatttattt
    12721 tgcaaacaca tgatttccta ttctcatata aggattcaat ctatttatcc atttgcaagt
    12781 ggctatttct ggattccaga aatatactgc tttattagtt gggtcagttc cataaagagc
    12841 ttcttgagct gctttacgtg ctgagtctgt tggtgccatc tttattgaac catcaagcac
    12901 aactgagaaa gcattttttt gatatataac ctcttctatt gtgtctggaa atctagggtc
    12961 caatactcta tttattacta cagctgctac agcaacctgt ccctcatagc tttctcctct
    13021 agcttctcct gcaaccaact tagataataa catcaagtca ttatcagata aatttattac
    13081 ttctttcttt gctatatttt tttcttgttt gtttattttc tcaatctttg ctgttggttc
    13141 atatgcaaat acctctttaa taggcataaa tataaaagtc aaagctaata gtgtagccat
    13201 cagtttactc cacctgtcca tgattaaaac cactccttac attcttattt ttaaaatttt
    13261 ttctctaaat ccttcactac ttcaattact ttaaatttaa tttttactct acttgttttt
    13321 tttggtgtat cttgttttat taacttatat tctttatcag ttagaacttc cttcaactta
    13381 tcatctgtag atttatctat tactccatta gattcatcaa caaagcatag tggagggaac
    13441 ataacacacc accaattttt tccttcacct tttccaatga taatctttaa tgccttatat
    13501 tcaccagctg gaagaactat attagaatat tgtttagtag gaaaattgct atattgtata
    13561 cctaccttta cactgtaatt atatccattt tcaagaataa tatttttact tatttcttcc
    13621 agactactat attccttttt tataatagct tcactttctt ttatactttt tgaattttga
    13681 agctttggtt gtaaatattt tataacttca tctctaacct ttagtttaag ttcttggtct
    13741 tcatctgtat tactatttgc tataacatga aatcttatta atttatcttt gtaatccttt
    13801 gatatattgt ctactttttt tacctcacca tttattacta ttgtcattat tgatatgact
    13861 gatataagac ttaatattaa tagtcccaat ctaatcttaa tttttctcat tgaaaattcc
    13921 ccctttaaga taaatttata tcatcaatta tgtccaatta aatttaattt attcataata
    13981 ttttttaata ctaataaaaa aagctatctt aaaatgatta caatcatttt aaaatagctt
    14041 tttaaacctt atattttata agtctttact aatcatagcc ttatctatca taaactgctc
    14101 tgctttttct atatgctctg tagcatactt cttggatagc tcactatcac ctttaattat
    14161 agcatcaagt aaatatttat gctcttcaac aatattatgt gatgtatcat aagcagacat
    14221 ataggtaact ctaaacctat atacttgttc ccataatgag tttattagtt ggattaattt
    14281 tttattatta gttgctttaa atatacattc atgaaattct acatcaagtt ttaataacgc
    14341 atctacatcg gattccaaag tgcttttttt aaattcttct gatatatctt taagcttttt
    14401 aatatcttct tcactaattc tttcagcggc aagatatgct gctaagcctt ctaggcttgc
    14461 tcttacttct aatacatcca taatatcctt taatgacata tttgccacat acgcaccttt
    14521 tctaggcaac ataacgacta aaccttctaa ttcaagcttt cttattgcct ctctcactgg
    14581 agtcctactt actcccaatt gttctgctag ttgcacttcc attagccttt gaccaggctt
    14641 taattttcct tctaagattg cttctcttaa attctcaaag actacatctc ttaatggctt
    14701 gtaattatcc aagtttagtt tagttaaatt ctccacaaat ttcaacccct ttttgactgc
    14761 tattaacaac atacacctgt ttatattttt ttaataattc tttttttcca attagagcat
    14821 cttctttatt tttaaatagt ccaaaaactg taggaccact tccactcatc atagaaccta
    14881 aagcattatt tttcatcatt atttgtttta tatcacttat ttctttgtgt ttacttatag
    14941 taacattttc aagaatattt accatgcttt ctgatacagc ttttatatct tcagatttta
    15001 aacactctat caaatattta ttatttggtc tttttttaat attattcaaa tctaatcctt
    15061 gataaacttc tttagtagaa acgaataagt ctggtttgca tatcaatata tttgtatcac
    15121 atggtaaacc tttgatgctt gttagctttt ctccaatacc ttgagctagg gcaggtcttc
    15181 ctgatataca aaaaggaaca tctgcgccta aattaagtcc tatttctttt aattcatctt
    15241 cagataatct aagtttccat agttgattta aacctactaa tactgcggct gcattagaac
    15301 ttcctccagc cattcctgca gctacaggaa tatttttttc tatgaatatc tctacaccct
    15361 tttttatata aaatttattt ttaagtattt tggcagcttt ataaacaata ttatcttcat
    15421 ctaaaggaat atctaaacta gtgcttttta cctttatttc atcttcttct aattctttta
    15481 tctttactat atcgtataaa tctatcgttt gcattatcat ttcaacaaaa tgataaccgt
    15541 cttgcctttt tcctaagaca tcaatggata aatttatttt tgctctactc tttagtctta
    15601 taaaatccat tctatcctcc ttgtttaaag aataatgcat cactaacaat aatattatac
    15661 tacaaaaaat aaaataatag ggtattataa taccctatta tacttaatta ctttaaattt
    15721 tttaatatta atctactaat acgacttttt tttctaaaat aagacactta ccaggcttaa
    15781 ttggttcatc aagttttata tcattaagct ctgcaatttc gttttctgtt gtattgtatt
    15841 tttttgctat attccaaaaa gtatctcctt ctttgcatat atatacgatt atgctaggag
    15901 ctttagataa atcatacaca ccttggtctt cacccttgat aataaaattt tctgctttct
    15961 tatctaatgc ttcagtaaat ctcttaattt taataatcaa gtctatctgg tccctattca
    16021 aatcaacttc aactttatca atacatgctg tattaaacac agatgctgta tcagttagat
    16081 tatccatttc tatatcatgt tcaaaaggaa tttcctcact aattttataa accagtttta
    16141 aaccttcaac tggtacaaat aaaatttcaa cttttatgat tccttgtata atgcttttat
    16201 ttccttcaat ataactattt tctatagaca tggttggaca aacgcttaca atatctttta
    16261 tttgtatatc atcattatca tttcttatgc cttctctaac cataaatgtt tcttcactac
    16321 gtcttaaagt cttatttaat tgaataggtt tatggtcaaa ttttattatt ttttgaggcg
    16381 aatatgcatc ttgtaatact tctcttgtaa cttcatctgt aacttttacc ttacaacaag
    16441 ccatacaatc tatttctaaa agacctgtgt tactttcact attttgctta aaaatatgat
    16501 taaaatctga catagacaac aatacttctt cagtcatacc atcactaacc ccaggtactt
    16561 ctacaaattg ggtaaactca atacctactc tatctaactc tacaagttca ccctcgtatg
    16621 tacaagctaa tggatttatt tctaatactc caccaataat aactttattg tcagttactc
    16681 tactttcttt tactttaaca catggattta aacttattat agattgaatt tcctctgtgt
    16741 ttattgttat agtatctctt atagaacttt ctgccttttc tattccaact atatcttgaa
    16801 aacaaatttc ctttctgtgc ttttgtatgc cttctacctg tgccacatct tttacaatgt
    16861 ctagcctttg cttttcaaac aaacttcccc ttatattcat aagagctcct actttaattt
    16921 ttctctcatt cattatagta caatccatat gctctacttc tgaaaataac atgtattcca
    16981 tatcttgaac aacattgtct ttttctatta cttcgtttat atctactttt ccatctacat
    17041 tagaaatagt atttttatca tctgcaatat agattacatt ataattaaaa cttcctctgc
    17101 aaagtatctt cccatctgcc atttcaatct ttttaagtga tatgtatcct tctgttttta
    17161 caatttcata tacatctaat tttttgtctg gtacaactgc ctccgcttcg ataaatgttt
    17221 gaaattttcc aaagtctatt ctattgtcaa ccttaattac atctttaatt aattccatag
    17281 tctacctcct taagtattat aaaaatatca cactatattt atatttaagg agcaaaaaaa
    17341 aaattacacc tatttttaat aggtataatt ttaacttatc tgtaatttat cttgatctct
    17401 aaaaacttgc aactttacat tagatgttaa taaatctgag taactataag ataccctagg
    17461 atatccatta ctttcatcat ctaattttat aacaaacacg cttggataaa ctttttctag
    17521 tataccctct tttgtaataa tctgctttct tcctttatta gctttcaaca atattttctt
    17581 tcctatatgt ctctctaagc ttactcttat cttatctaga gtttgaacag tagccacaat
    17641 atcaccttct ttaacgttta tatgtttaca taatatcaca tataaacatt tttgtcaaat
    17701 gtttaaacta attattttat atattttttg caaaaatgtc aatactattt ttaaagatat
    17761 acagtataat tatgctaatt tttgctaatg taatattgtt tttggcattg cccatctaaa
    17821 ttttcctctt gtttcagaac caccaatccc cttaactcct gtaatagtct gctctataac
    17881 ctcatcaata ggcactacta catcaattct tgaacaagca attttctcca ctataaaaac
    17941 agggtctaat gcatgtatca ttattcttcc aggcgaactt gcatagtttg ctcctgcact
    18001 tattattttt tcataatttg attggcatgc tcctgcaaat atgacaagat tatccaaatt
    18061 tggttgccat ttgcgtgctt cttttactgt tttaacaaaa tttgaagagt ttctatagtt
    18121 actcatatct tctatatctc ctctttttat tgtcattgca tcatgacctg ttattactag
    18181 tatatccgga ttatattttt ctagtagtgc tcttacttct ttatactgat tttgttctga
    18241 aacagctaca cctactgcag gtattccaag ttgagcatat acatccaagc aaatctttaa
    18301 atattcttta tctccatcta tctgtaatac ctttccaggc attccatatg tgttaggatt
    18361 taattgaagt tttggaactg ctcttgtcat tcttttttgt ctttccttag cctttcttac
    18421 tgacttatat aataaatttt ctacattttt atctattaat atatctttta tatcaggtgc
    18481 ttttactaat tctaggtcat caatataagc atctgctatt actctaaatg caattccctt
    18541 tagtatagct attttctcat tattttcatc tacaccaaaa gagactattt taaatactat
    18601 gtctttgtta tgtgactttc tagctacaat gtctcctacc ttcatagtta aacctccaat
    18661 aaatttttat cttagtttat actatgttcg atataaaact ttgtttccta atgtcaaaaa
    18721 aacatttctt ataatacttc ttttattact cttaaagcat ttttatagta tatcttttct
    18781 atctcatctt cactaaaccc ttcttttttt agaggttcat acaatttacc catctgagat
    18841 atatcttcaa tctcaacttt tgaatctatt ccatcaaaat ctgaacctag agatacaaca
    18901 tctattcctc caacatttac aatatgcttt atgtgtctaa ccatatcttc aagtttactt
    18961 tcgcttttat cacttaaaaa taagtggaag aagtttatac ctgtaactcc acctttattt
    19021 gctaatacct taatcatatc atcagttaaa tttctagaat gattcatcat tgctcttgaa
    19081 ttagaatgtg tagcaattat tggtttagaa gaaatcttcg caatttcata aaatccacca
    19141 tctgaaatat gagaaacatc aactaacatt cccaattcat tcatcttatg tactacttct
    19201 ctaccaaaat cagtaagccc tttttctcta tattctaatt tattatgagg aaaactcaat
    19261 tcattcacat gattccaact tattgtcatc attctaactc ctaagtcgta aaatttattt
    19321 aagttttcta atttaccttc aagcactgca ccttcctcaa tagataaaag agctgtaagt
    19381 ttaccttctg attggttttt cataatttct tcatagttag tagctaaagc tatttcatca
    19441 ctattttttt tcatttcctc atgaaattta gttgccatac tcatgcaata atcaaaagga
    19501 tgttttactt cttcagtatc aacaaaaaga gcaaatgttt gagctaatga atcccccttt
    19561 tttaatctgt ctatatcaac agaatattta ttagacttaa gctcacttgt ttctacattt
    19621 tccattaatt ttgctatagt atcacaatgt aaatcaataa atttcatatt tataccccct
    19681 gtatatttta tatttatatc taaaaataga aatagttgtc cttatagaca actattgaca
    19741 actatttttt aatattcttt ttgatattac ttaatcctat tcttctttta agttaggtat
    19801 tactttttta acttcttcaa tttgatgcca tgttttatct atattatttt cactaatata
    19861 tttttttaaa tcagttaaag atttaaaatc tttaactgat aaattgtcat catcatctgc
    19921 aaatcttaaa ataaacatga gactacctta ctttccacaa catttcttat attttttccc
    19981 acttccacat gggcatgaat catttcttcc tatttttgct tctttaacta ttgttttaga
    20041 cttcttataa gcctttatta tttcttttct ttggtctgct ggtagtatat cttcccaacc
    20101 ttgtaaatta tatagccaat gggcttcaac agttaccata ttgttatata atttttccca
    20161 atctattttt atagaaactt ctttatcagc ttctacatct tctaaaacta tatcttcttt
    20221 taaactttca cttattccat ctatgaatcc catgaaaaat tcattagtag ttccaaattt
    20281 ttcggctaat tcgcttactt taccttcaac aacatccatt ttattattta atatttcatt
    20341 gtatattcca gcttcaactt tcaaatattc ttcccagaat tttacttctg cttcttgatt
    20401 ttcatgatta tcacttaaat ttctccattc agtatataaa ctcatacttt ttcctcctaa
    20461 gtatacctat atttttatag tttttatagt ataaaatctt ttagttagat tggaagtttc
    20521 ttctttttga ttataccata ttctatttaa agttaactct tatattatat cataattaaa
    20581 caattctttt aatactttta tagcatactc ttggtctata aaataagggc ttgtcgcaat
    20641 tgttattact tttgcttttt taattaaatt ttttatcttg taaaatttga gttcaaatgg
    20701 tatatagtcc atatcacttg aaaatatatc caaatctata tcaagaacaa actctgattc
    20761 tacatctaaa ccaaatccat ataggctatc tattattatt aattctgaaa atatgttata
    20821 ttttaaggca ggttttataa aacttcccac atttaaaact tcatttgtat acctaaatac
    20881 atcttttatg ttatttatat ctacattata gttttcaggt tctctagtat ccttatgttg
    20941 gtctacatgc actaacttac atccttttgt aaactcaccc tttaacaaag attttatcca
    21001 aaaataaaat gcatggttat gattatcaaa tacatatatc tttttatttc ccaggacata
    21061 ttcgaccata ttctccaatc cttttgcttt tatttcctta ttttcatcta cttcattaaa
    21121 gactacctca ttgcccaatc ttacattatt taatgtacca tttataagtt ttggaacata
    21181 tatactctta ttttctcttt tatcatacga aaatacgtta tttcccaccg gtttttctat
    21241 ataaaatcca ctatattcat aattttccat aaaactttac ttcctccatt gttataatat
    21301 ataatctata attttaaaaa ttaaaatgca cttattgtaa atcatattat ctactataga
    21361 aaacatctta tataactaaa tataatatct tctctcaata tgtattatac aataagtgca
    21421 aaatatatct atataaaata tactacattc ctataacaaa accatcagct tcaattggaa
    21481 ggtaactaac tcttctattc aactccactt ttccaaaaaa tactacccaa tatggtctaa
    21541 aaatactttc aattttgact atttcaatct ctgggattcc acccataaac ctatgagtta
    21601 gtctcatagc tattttattt gcctttgctt ttactcttcg ttcatcctta tcactatatt
    21661 gcacaatatt acaatccaca ttttttataa tttcatatgt aggaaaatct tgtactatac
    21721 taccagaacc tgtagagcca ttaactaaaa ttttcaattc attcacacca ttagaatatc
    21781 tcctactctt gaaaatggat ttagttttgt gaattttaag tgtgacaatt ttaaactcta
    21841 tataatgaag tctaacttca tgagcatatt tattctttaa tattagctta ctaaataaag
    21901 aattttttcc acttttagct atatctatag cttcttcttt tgtaattttt gtaggtaatg
    21961 cccttatatc cataaaatca ttctctttct tatttttttc ttgtcatttt tgaaactcta
    22021 tatatataat caaaaaatat tataattaca actgccataa ctccatatgg aattattctt
    22081 ccaactataa tatcatttct agtcaatata ccataaaata taaatcctgt tattaaaaag
    22141 tacaataata ctagaaaacc atctattcca agccatttta tttctaaatc ttttttcacc
    22201 ttagatacta atttagacat agcagtttct ccttttatag attattttgt gtatttaaat
    22261 tttcccctat tctattgcca atcatccatg caataaatga aaaaggtata gctatcacaa
    22321 gaggttctaa ctcaaaactc tgaactggga atattttaaa ataaccaaaa gtcactccac
    22381 ctacaatcat tcctaaaata ccacccatag atgtccttct tccatttcca agaagtccac
    22441 ctactatagg tacaaatgct gaacccatcc aaatacttcc tacaaatata catgcatcat
    22501 atacattttc aaatctaaat gctacactaa gacccataac tgctgctaag catacaccta
    22561 ttctagtaaa ctttactaat tgtttctgag ataactcttt ttttgtaatt cttccatata
    22621 tatcatttgc taatgtagct ccagctgcta gataatagct atctaatgtt gatattatag
    22681 aacctaaaat tcctattata aatattactc ttattccttg tggtaatgta tctagaacta
    22741 aagttatgta tccagcggct ggagccattt ctggataagt agctcttact ataagtcctg
    22801 tcatagttaa caccatgtca aaacatatcc atagtgaaaa acaagttaat aaagctcttc
    22861 ttccaacctt aggagaatct gctgctgaaa atctttgata aaaagttggg tctgaataag
    22921 ctccaaaaga atatacaatt aacatcaatg ctttcatagc tgacatacct tttgtagggt
    22981 gcataagctg tggtgcatct ttgagtgctt ctgtgagacc ttgaaatcct cctatttgtt
    23041 caaattggcc tggcataacc atagttaaac caaataccat acagaaaaac aataccatat
    23101 ctgtaatggc aactcccata agtccaccaa gtactgtgag tacaacaact attcctacca
    23161 ttattaagcc tattaattct ttatctattc tccatgctac gttgcctata gttcccatag
    23221 cagttatttc tccaagttgt gcacaatata taaacatcaa aattgcccca attatagcaa
    23281 catgcttgcc ataaaaactt tccagtaaat caggaactgt tatattggtt cttatatgaa
    23341 tccttggacc aacccacaat gctaaaggca ttctcccaat atgtgcagct attgaccaaa
    23401 ctgcccatgc agatattcca tatatagctg catactctac actacctact gtaccaactc
    23461 ctccatacca tgatgcaacc aaagttccta ctatcaagga ccatggtaaa cttcgatttc
    23521 ctaggtaata actttccata tcttttacat ttctagaaaa ataataacct attcccaata
    23581 atataaatgc aaatataact ataatcccat tctcccaaaa tgttaacata tctaatctcc
    23641 acattcttat attttaattt ttaactttaa tatctaaata tgaacaactc tacccatttc
    23701 cataaaaata ggtagagtta tataataatt ataagtattt aaacaatctt gttattaagc
    23761 aattttattg ttcaagctct tattttctct tttatttatc ttatttaaag cataagtagc
    23821 aaatatacct ataactgcac atactacaga taaaataaat atatatgtat atcctgtttg
    23881 tccatattta tctaaccaat tacctgcaat agtgtaataa aatgcatctg gagaaaaccc
    23941 tataaatgaa gcaaatccaa ctactgctcc tgtagtttct aaagatatat tagactctga
    24001 aacagatgca aagtatatac ctctaaatcc aaataaaatt acacttagta ttatcatgtt
    24061 tattacagct atccatataa atcctgtact cttaggaata accaagtaag cagctgtact
    24121 tactgtcatt agtataaatc ctacaaacat tactcttata gatgacccaa ctttatctgt
    24181 tattacacca gcaactggag atgctcccat ttttataaca taagttctaa ttattgatag
    24241 agcagaaact agagtcattg gcattacata aacttctgat aggtatggac ttaaataagt
    24301 taaacttgag aatactaaat aagttgaaaa tattataaga ccaattagcc aagcctttgg
    24361 cattgtaact acctttagca atgacttaat gcttaatcct tcatctgatg ctccactatc
    24421 ttttttctct tctactaaga aaaatagaag taaaccacat attatagatg ctgcagaata
    24481 taaccatact acatatttga atcctactgt tatttcagca aatttaccaa agaaataaag
    24541 acctataaaa gaaattaatg tacctgatac acctgataat ccttcataaa aaccaaatat
    24601 tcttccttgc tctgactcac ttccttgcat tctaacaacc ttaacagatg ctgagaaaaa
    24661 agttaatatg gttgtaaatc cccataatac aaatattaaa agtatcatac tatatgatgg
    24721 gaatgtagaa aaataaaacc caagcgctcc tgaagcaatt aaagaaaaag ttatcagctt
    24781 tctaactgag aatctatctg caaaccatcc tcctataaaa taagatacca ttcctactaa
    24841 tccatataaa cttaataagt tccctatttg tgtatgactc aacgataaag cttgttgcat
    24901 tgggtcataa aatgatgatt tcatatatgg taaactatac atagctgttg ttccaaatgc
    24961 caatatgaat attatcaaaa atcttttaac attacttttg ttttccattg atactctctc
    25021 cttaaaattg tattgtttat aatctcattc atgtaatttt attttaattt ttctattgct
    25081 tgtttaaagt catttattat atcatttgaa gattccaacc ctacagctat tcttattaga
    25141 ttttctccat tcatttgaac caatgtatgc tcttccccta agcttgttgc tattgtcgca
    25201 agctctaaag agttcattaa tcttctacta gcttggtctc ttgttaatcc attgatacca
    25261 tccttaagct taaatgaaac tacacctcca cctaaaccat tcatttgagt tttacaaagc
    25321 tcgtattgag gatgagattt taatgctgga tattttacta attccacctt ctcttcttcc
    25381 tctaaaaact ctgctactgc taatccattt tcacaatgct ttttcatcct catatctaac
    25441 gttttcatac ctcttaaaat catccatgca ctcattggtg gtaaagacgc tcctgtaaaa
    25501 caacataaac ttggccatct aactaaatct attaattctt ttgaacctgc tactgctccc
    25561 cctaaagtat ccccatgtcc atttataaat ttagtaagac tatgtattac taaatctgct
    25621 cctaaatcta ttggtctttg cacaattggt gtaccaaaag tactatcaac tatggctaag
    25681 cttttatttt catgagcaat ttctgctatt gtttttatat ctactaaatc taatgatgga
    25741 tttaaaggac tttctatata aactatctta gtatttggtt tcattgcatc ttttacattt
    25801 tgtggattta ctgcatctac aaatgtaact tcaactccaa atttagataa caactcagac
    25861 ataaataatt tagtgtgtga gaatattcca tctgaactaa ctatatggtc accacttttt
    25921 actaatgcta atatagtaga tgttattgcc cccatacctg acgtactaga tatagcactt
    25981 tctgcatttt caagcatagc caacttagct tctaactccg taagagtagg attacactct
    26041 cttgtataca catatcccca tgtcatacac aactcttcaa aatgctctac tgtctttgca
    26101 gcaaatgttg ctgtttgaca tattggtgta gccaatgcac ctgttgttgg gtctgcacta
    26161 tgtccccaat gtattatttt tgtctctaaa tcttgattaa tattgttact cattttgatt
    26221 tcctcccatt ttgacttatt tatacaacgt tttccctacc aaatttcatc aatttagttt
    26281 agtcttaata cattttttca agtatacttt tatatttgtt ataatttcta aaaatatgac
    26341 acttattttg tctttttaca gttctaatta taaactctat agtaactata gagtcaatat
    26401 gtttttgata ttttttttac aatgtttaaa atagttggta caaaaatagg tatatcttag
    26461 aataattaaa gtcatcctaa gatataccta ttttcatacc ataatttaaa caattatata
    26521 aagcattaat ttttttacca taaataagtt tttcaagagt tatcatcttt taataaatat
    26581 attttagtta aatattttaa tattatttaa tctctccaac aaaatacact ctaaaaacaa
    26641 aattatataa ttaaaaaaat atcctataaa aagctaaaaa gtttttctat ttactacaac
    26701 taaaaataag atttgttaga ggtataaatg tatttagttt atttacacct ctagtatttt
    26761 acaaattata cctctactga attgattttt tagcacgaat aagtgtgctc tttgagatac
    26821 cagtcatctt cacaacttct ctatatggtt tagaatttaa taatcctaat gcatattgta
    26881 tctgttgatt gctgtatttc tttggcctac catctttaaa gtcctttttc ttcctagcaa
    26941 attcttttcc taattgagta cgttctatta tcatatttct ctcaagttct gcaactgcaa
    27001 gtaacgtagt caaaaagaac ttacccattg aagtattttc taagagacct atgtttaaaa
    27061 tatgcacact tacttcttta gcaaataaac cttctattag ttgtatccct tctgatgtac
    27121 ttctagctaa tctatccaac ttagttgcaa caagtaagtc tccctttttt agagatgata
    27181 taattttttc aaatttaggc ctatttgtag tagtgcctgt ataagtttcc tttattattt
    27241 tagcatcatt atatttattc aaaatttctt gctgttgctg atacaaactg ttgttgtctg
    27301 tttgattttt tgtacttaca cggcaatatc catatataat aatcatcact tcctaacata
    27361 aaaattttgt acagaagtta cccagaagct tgtttttgct tatctttatc cattttacta
    27421 aaacggtttt ttagtgtcaa tactgttaac ttttgacacc ataataattc ctttataatt
    27481 tgtttgacta tgtcaaaaat taccaatata atgaaattaa tgagtttata caaattggag
    27541 gaaataatat gaatagtctg ttaaaagaag cgatagatga attaaaaaac atccattgcg
    27601 gagaagtttt tttattaaaa gaattgttca aaggctatga atggaacagg ttggagatga
    27661 acataaggtt gaaattagga aaactttttt tgtatgaagt ggtaactaat cccaatttaa
    27721 atgtagaggt catcaataaa aactctgctt atcagcaaat gtataaaaaa aaatagtgac
    27781 ttaaatttat ataatagtat aatcttctta ttgattcaaa agttaaaact tttaatacaa
    27841 aacaatcatt ctattttagt atttatcaat atgtctaaat ttatataatt aacacataaa
    27901 aatagtactt gtttaaatca cattctttaa gatagataaa tcttaaccct actccagcat
    27961 aaatactaaa atagggctta gaattatcta tatttttatc ccataaaata cttaactctt
    28021 cctcttgatt gaacatatat atctggggaa atacaataaa aacgtgaatt tataaaattt
    28081 ccaaagtcag aaaacttcaa cttaaactta ggtataccaa aactttcagg tgctatttca
    28141 tcaaccccaa aataaattac aattccacta tctgtaagat aaaaagcttg gtcttcaggt
    28201 ataactaatt caacattaga ataatacatt tctctatttt catttatttg agatttaatg
    28261 tagtcagtaa ttatattaat ataatcaact ccatctctaa aaatatcgtt aagtaaaagt
    28321 tcttttccag ttgtcaaatc tatattgtaa ttattcaatg tattgtattt aggcccaaag
    28381 ttattggcaa atcccataag gcttaatatc atactcagta cataattttt attaaaagta
    28441 actgcataac ttgtagatac ccagtaactt tgttttggaa gtgaattttg agaagctgcg
    28501 ttattatatt cttgttcttc ttcctgcaag ccatttttaa aattatttac agtcattctt
    28561 acagcattgt ttatgttatt taaaataact gggtttacat acgtagattg atttctaata
    28621 ttgtatataa caggatactt tacatcatat gaaaaatact tacttctttc aaaatcttca
    28681 ttaaattgta catataatat attatatgta caggtatttg tagattgata attttccatt
    28741 aaaatcctcc ccttttataa aaaaatcaga tacaatataa tatgcttaaa acagtaataa
    28801 tgttaatagt aaatgtaaag aatagaaagt atctaaaaca gcaaaaaatg actaattttg
    28861 tggtataata cataggattg agagttattt tataggagga aaaatatgtt attattagca
    28921 gataaatgga aagattatga acttatagat atgggaaatg gagaaaaatt agaacgttgg
    28981 ggcaattatg tacttagaag accagaccca caggttgtat ggccaatgga atctgaatgg
    29041 gctttatgga aaaaccctca tgggcactat cacagaagca ataaaggtgg aggtcaatgg
    29101 gaacacaaaa aaagatatcc agaacaatgg actataaatt ataaaaactt aaagtttcac
    29161 attaaaccaa ctggatttaa acatactgga ttattcccag agcaggcagc taactgggat
    29221 tggatgatag aaaaaataaa gaaagctaac agacctataa aagtgttgaa tctttttgct
    29281 tatacaggtg gagctactgt agcatgtgca tctgctggtg cagaagtatg tcatgttgat
    29341 gcggcaaagg gtatggttaa ttgggcaaaa gaaaacatag aattatcagg tcttaaaaat
    29401 caaacagtaa gatttatagt ggatgatgtt gtaaagtttg ttgaaagaga aataagaaga
    29461 ggtaaaaaat atgatgcaat aataatggac cctccttctt atggaagagg tcctaaggga
    29521 gaagtgtggc aaatagaaga aaagttatat agttttgtag atttatgtat gggagtttta
    29581 tcagataacc ctttattttt cttgattaat tcttatacaa caggattttc tccaatagtt
    29641 cttgaaaatg tattgtctac aacaataggt aaaaagacaa atacaggata tatatatggt
    29701 ggggaattag gaataccagc ttccagagat ggtaagatac ttccttgtgg tatatttgga
    29761 agatgggaga aaaaataatg ataaaggtca tatatgaaga taaccattta ttagttgtag
    29821 aaaaaccagt taacatatta tctcaaggag ataatactaa tgataaagat atggtaaatc
    29881 ttttaaaaga ctatataaaa aaaaagtata acaaacctgg aaatgtgtat attggtttaa
    29941 tacataggtt agatagacca gtgggtggag ttatggtttt tgcgaaaaca tcaaaggcag
    30001 catctagatt gtctgagcag gtaagaagta agactttcaa aaaaacttat ctagctgttg
    30061 ttcatggcaa gataaaatct aactcagata tactaaagga ttttttatat aaaaataaaa
    30121 aaactaatat ggtaactgta gtgaaaaaaa accataagga tgccaaagaa gctgaactta
    30181 actataatgt cttaaacttt aaagaaaatt taagcttagt tcaagttaat ttaaagactg
    30241 gtcgctctca tcagataaga gttcaatttg caagcagaaa acatccattg tatggagacc
    30301 aaagatacgg caaggaaata aataagattg gacagcaaat atctctttgg tccaataaga
    30361 tagaaatcta tcatcctact acaaaagaaa aaatggaatt tacatgtcat ccccctgaca
    30421 aatatccatg gaatatattt taagtctcaa atgaggtgat tatatgagca aaaaattttc
    30481 aataggagaa atgtccaagc ttcataaaat aagcatacag acactaagat actatgacca
    30541 aataggttta tttaaacctg ttcatgttga ccaagaaagc aattatagat attactcaat
    30601 tgaccaattt tcacaattag atattataaa atatttaaaa tatttaggta tgcctttaaa
    30661 agaaattaaa tcaaagcttg atgaaaataa tgaagaaatt ttaaagctac taacaaataa
    30721 aattaatata gtggatgaaa aaattaaaga attagaactt ataaaaaaag tactaagcgc
    30781 aaagaaagag accatggaaa aaggtatgaa agtagatgaa gttggtaaaa tagtgagaaa
    30841 acatattcca cgaagaagta tcttatctat agagtatgaa gaaggattaa gtagtgaaga
    30901 aactatggaa ctttcaagaa gacaaatagc aaatatatta gaagataata tatctgtatt
    30961 ctatggtggc gtaagtggac ttgtatctat agataaattt ttaaaggaag ataaagttat
    31021 ttatgacaat agttttgtta ctgttgaaag agatttattt gatataaaat cacaaaaata
    31081 tataacggaa atttcagctg gtgattttat ttgtatgaca tataaaggac cttacaaaga
    31141 aaattatacc gcttttaaga agctagttga ttatgttgaa tataaaagaa tacctattga
    31201 aaattatata tatgaaatac ctataataga ccctctgtct acaaacaatg aagaagaact
    31261 tctaacagaa attcaaatac aagttaaaaa gagagaaatt gacttaagat gtctagtgta
    31321 gtaacctagt ttacttacaa atcttaatat aattgtctat tatcaacata cgattttttt
    31381 agataatgat tcctaacagt gagattttca aaaaataggc gggagcatgg ctaatgtcaa
    31441 ttcccaccta tttttaatta agccatgtca tcacgaagtg catcaataat attttctttt
    31501 tttatcttat taatagtata caacattgta ataaatacga taaatagaac actaaatata
    31561 cttattccaa tactaaccca tggaatcaca aaatcgatat catctgtaac cattacttta
    31621 tgaattagcc atgaagaaat tactgctata ggaagaccaa agaacaacgc cctcatacca
    31681 taaaaagcac actcaaaatt catcatcttt tggaaatcat gctcagacat tcctacagag
    31741 cgaagcatgg caagttctct acgacgtaac ttaatattag ttgaaattgt attaaacaca
    31801 tttgcaatcg aaatcagaga aatcataatt atgaaagtat acgcaaaaac attggcaata
    31861 aaaatgtaat tacggttctc atccatcatt ccatacatat tgtacagatt gtactcattc
    31921 gtaactccct cactctcaat aatttgctcc atttctgaaa ctgactgagt tggatttttt
    31981 gattgaaagc ttaaactttc aattcctgta gaaacaccag gaatctcaaa cttatcttta
    32041 gctgaataag gagccaatac tctgaagctt gctttcttca tatctgagtt tctgattcct
    32101 ggaggagaat caggtggcac cactttgaca aatgaaatat ttacattttg tctctcaggc
    32161 gtgtccaact tgccattaac ttcaggagtg aggctaacat ccatagaaga attggtaaaa
    32221 atatcaataa tatgattatc tttcgttaac atagaaacgg caatcatttt tgcattttgc
    32281 ccagtatatt cttctgttgg caatccaaga cttttgataa tattcagata agttctatca
    32341 tcaagaaact gcatatctac tggcaattca agcatttcac tcatagaatg taaaccatta
    32401 tagttcaaat aatcgtcagt aagctcactt actttggcaa cacatgaata ggtcatcagt
    32461 ttctgataca aaccttcgta aacaccactt gcagttttta gcttatcata aagcttcaac
    32521 atttcgctgt ctttcatgcc ttgtgtagaa aaaataatgt cttggtcagt aacctctgac
    32581 ctctgatttg acatctgttt taggtctttt actaaagcac ttgtagagat gaatagaact
    32641 atgcttaaaa caagcgacaa tacaatactt cgataacgtt tcttgtttct tttaaagttt
    32701 ttcatagcaa gaactccctc aagaccataa atttgctgtg ttagctttga tgtcttcaca
    32761 gatttagatt caatttttac ctcatttgtc tgacgaatac actccatcac tggtgtgttg
    32821 gcagcctttc ttgctggtat gtaagcagaa attaagattg taaccatgct taccactgct
    32881 gcaacgacga ttgcaggtat agataatgtc agtgttaatg gaacattgac gtagagaata
    32941 tttgcaaagt ttttagctat tacagaaatc acaagtccaa tactgactat accgattaca
    33001 acaccaattg gtataccaat cacaccaata caaattccct caaataacac tgaatttcgt
    33061 aactgttttg ctgtagctcc aacagatgaa agtattccaa attgatgtgt tcgctcgttc
    33121 aatgacatat taaatgaatt atatattaga aaaattgagc ctagcataat tatggcaagc
    33181 acaacgccac caaccgagta taaaaacata ttgaacaaat tattatcaga aatacccatg
    33241 aaacgcaaca cattatcatt gaaaatataa tcatgctctc tagcactact tgtataagag
    33301 cgaacatgac gtggattttt caatgtgaca aaaaggctaa gactgtcagc ctttacttgt
    33361 tcatcttctt tggttatcaa ggtatatcct ggtgcagaag attcttcaaa tacaggcttt
    33421 ctacagatac ccacaactgt ataagttctc ttattttgtg gaataaaagc ttcactccca
    33481 gcagtgtatg ggtcactctg accaagattt tcattttcac tcattcgttt ccctacatcc
    33541 aaagaaatag tatcacctac tgcaaatttg acaccaccat ctgtcgcaac ttttcctgaa
    33601 acaagaatct ctctactatt ttcaggtaat cttccagaaa ttaaagttat aggcaaagta
    33661 tcaaaagctt ctttgctgaa tccagcaata aaaagatatg gtttatctgg atttctgcta
    33721 ccctcaagtg ttgcatatcc aatattttca aatgttacag tattttcaac ttcatcattg
    33781 accctctgtt cctgtacaaa agaagaatca acatccaaaa actcgacatg ccaatcacca
    33841 tattttataa tggcaccatt tatcatatag ttcatcaaag aaactccaaa ggtagtgact
    33901 gctgtaatca tagcagctga caaaacaact cctataattg ttacaaaagt tcgagtgcga
    33961 ttctttttca acccttgcag agcaactttg ttaaaaatat tcattaacgg ttcaccttcc
    34021 tctcatcacg cactactttt ccatctgaaa tacagataat tctatccgcc tgtaaagcaa
    34081 tgttttcatc atgtgtgaca atgatgagag tttggtgata tttactatgg ctttctttaa
    34141 gtaactggat aatttcttgt ccatttttgc tgtctaaact acctgtaggc tcatcagcaa
    34201 gcataactgc tggtgcattc atcaatgcac gaccaatagc aacacgttgc tgttgacccc
    34261 cagaaagctg atttggtaaa tgtgtttttc gttcttttaa accaagtaac tctagcaaat
    34321 catttaacct ttcttggtta accttacgct tatccataag tataggtaag gtgatatttt
    34381 ccactacatt tagtgttggt ataagattgt gaaactgata aatcaatcca acttgtcgcc
    34441 tacgaaaaat agcaagtttt tcattgctct gaccatatac atcttgaccc tccagatata
    34501 tctttccact tgttggtaca tctactcctg caatagaatg tagtagtgta gatttaccag
    34561 aaccagaaga accaataatt gcagtaaact ctcctttttc aatagtaagt gagacatggt
    34621 caagtgcagt aacctgattt tcattcttac cataaacctt acataagttt tcaatcttta
    34681 aaaatgccat tatataagtc tcctttcata tttcataaat atatcataga actttcaact
    34741 tacatttaag tgacatttaa atgagagatt cgtcactttg gaaaacgaat aacaaatatt
    34801 gcaccacctt gtgggtgatt ttctgcagta attgttcctc cttgcctcat aataatcgtc
    34861 ttacaaagtg ccaatcctat tccataacca gctgtgcttg aatttttccc acgataaaat
    34921 ctgttaaaca gacatggtaa atcttctttt ttaaagcctt ctccattgtc atgaatagta
    34981 agttgagtaa acaaaaagtt gtcctcacaa acaatatcaa ttttcccatt atcccccaca
    35041 ctctccatgc agtttttaaa tatattttga attgcttctg aaagccaatt taaatcgccc
    35101 aaaatcttta ctccttttgg tatattaata tgtagagtga tattgtgtag ttccatagga
    35161 atcaaaagtt gatgaagtgc agaactaatc aaattatcca catcaatttc ttctttttga
    35221 aaaactacaa tacctgcatc taaacgtgat aattttaaaa gtgaagtaat cagccagtcc
    35281 atttgtataa gcaattcttc tgtttctcgt atcaactctt ttctctcatc ttcatcagaa
    35341 gtattctcta acagtgacaa aataagagtt actgatgtaa gaggagtgcg aagctggtgg
    35401 gctatatcgg ctaatgaatc agcaagatgt tctttttctt ttttcagtgc ataattttgt
    35461 tctctaatac gtaacgtcat tttagttatt tcactttgca aaatagaaag ctcaccctca
    35521 tctgattcag taatatacag atggttagca ttatgaagca caagattaat ttgttctgaa
    35581 atctgtgcaa tacttttgta tcgattttta gtaaacacaa aaaatgctgt tccaaaggta
    35641 gtggcagaaa caatagaaat tattccagtc atagtactga ttgcaaatcc cagggctaca
    35701 gtgagaatag taattaataa aaataaaata gcaaatcttc gaaactctct atttcgaaac
    35761 ataacttcct cccaatctat accctgtccc acgaacagtc aaaattattt gcggacttga
    35821 agggtcatct tctatcttct ctcgcaagcg tttgatgtag acagtcaatg tattgtcatt
    35881 aacaaactca cctgctgcat cccacaattc atcaagtaat ctcactcttg taataatact
    35941 tttaggattg ctaataaaca ccaataacaa acggtattct aaagctgaaa gaaaaacctc
    36001 attgccattc tttttcacaa caccggttgc cgtatctaca taaagcccac aaatttcaaa
    36061 agttgaccca aaacgcccac tttttcgtag tgctgttcta attcgcgcaa tcagttcacg
    36121 tggacgaaaa ggtttagtga tatagtcatc tgcacccata ttcagcccag taacaacact
    36181 tgattcatca ccagaagctg taagaaagat aacaggaata tcttgatttt ctttgatttc
    36241 tgtacaaatc gtaaacccat ttccatcagg taaagaaata tcaataagtg ccaaatcaaa
    36301 tttattctta gcaatctcta caagtgcttc acttcttgtg gatacatggg ttactataaa
    36361 tccttctgaa ttaagcaaaa gaataagatt tttggcaatt gctttatcat cctcaactaa
    36421 aaatatccgt ttcatttact ttcatcctcc tttattttat tagttttata tattccagca
    36481 gagtaataat acactactat tttacttatt attacacaat tttcattagt ttcttttgtt
    36541 cttatacaat ctatgcataa tacctccatt gatttattat atttcttata acagaaatac
    36601 acaagatagg ataaaaaaca taatgtaagt gcaactattt tttaattcac taattcacta
    36661 acttataaac acacattcca atgcacgcca cctccctgat gatgtactta tcaatctaca
    36721 tgtttactat ggtactccca tgttagtata cactttataa tcaatacttg aaaaactaag
    36781 ctcaatgttt tattatatac ttatatatac cttatattat aaggtatatg ataaaataaa
    36841 gtaggtgaat ctatggactt gaataaagaa gtacttaaag gtcatattga tactttaata
    36901 ttatcaatac taaataaagg ggattcatat ggttatgaaa tagcaaaaat agttataaat
    36961 gaaacaacct ttgaattaaa ggaaagtact ctctatgtat ctcttaaaag actagaatct
    37021 aagggtctaa taaaatcata ttggggaagt gaccaaggac ttggaagtag aagaaagtat
    37081 tacaatataa cagatgaagg aaaagaaaat ttagaattaa agattcaaga gtggaacttt
    37141 atacaaagcg ttatgaacaa atttctaaag agaggggaat agatttatga aaataataga
    37201 agagtattta gaagcactat ataaaaatga tactagcaaa gaaattcaag atttaaaaga
    37261 agaattaaaa gagcatctaa tagattccac taatgaattt attgagaatg gatatgacat
    37321 aaatgaagca caaaataagg caatagaaag atttgatggc agtaatggag tatcagtaga
    37381 attacgttca atctttaaac aaaaaataga acctaaaaaa gtactattac gaaaattatc
    37441 aaattcaaga attgtatttt taaatttatt tggatggctt cttgcagcta ctgcatatac
    37501 aataaataga ggttttggtc atatagctcc accatggata attatacttt taataatatc
    37561 aatagttata ttaataatct taactattat aattaaggga ttaaaaaagg aaatagaaaa
    37621 tgattacaat atatagttat acagttatat tgtaatatat tatttattct tataaacaca
    37681 tattttgaaa ggagcttttt atatggtcaa aaaaattcta ttagaaaaat ttcttgatac
    37741 ctcaggagtt tcatatatca atgaaaaatt gaatctattt aaggatacta aatggatttc
    37801 atttggcacc tatataatga ccgttgatag tttttgttat agagaggatt caaagcacta
    37861 taatccaata atgtctacct ttgattatga atcagagatt tttatagtta atgccttata
    37921 caaggaaaat atctattcta ttaaaagtaa aaaactctat aggaatgtat ctaaaggaga
    37981 taagataatt gttacaatta aatactctta tgataaatat gaaaatctta tacatgaaga
    38041 aattcatgac tatcatctct atcctctttc tcttgattaa aataaaaggc tatagcctct
    38101 atagctaacg cctttaattt aaactacttt gaatactata taattttcat aaaagactgt
    38161 aaataaattt gtgcaaaata tataaaattt atctacagtc ttttgttata caaaatttat
    38221 aatctcttat agaacaaatt tgccatttct caactttatc acttttactc agctatatta
    38281 taaactttta atttatcttt atttaaatgt ttcttcttaa cttctactaa ggcttttagt
    38341 gtatccaaag atgtcattat ttcagtagaa aattcaatag ctgttcttct cattttaaat
    38401 ccatctcttg tagaatcatt tcctttagtt ggtgtattta ttactatatc aacttgttta
    38461 tttcttatag catctaatat atttggtcta ggctcttcaa ttctatttac tatatcagcc
    38521 tctattccat tttctcttaa gctcttagct gttccttctg tagcaacaaa agtatatcct
    38581 aattctttca tatcttttgc tatttctata aactcatctt tgtcatgatt gtttacagta
    38641 gcaagtacta c
//
LOCUS       DAFNNB010000019        36527 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0019, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000019 DAFNNB010000000
VERSION     DAFNNB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 36527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36527
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0019"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(49..900)
                     /locus_tag="L3M60_000871"
     CDS             complement(49..900)
                     /locus_tag="L3M60_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HBN6225565.1"
                     /translation="MYFLGVDGGGTKTTFTLVDEELNIVGTITKGTCHYNQIGFDNLT
                     KLLITGLEEVCKDAKINVEEITYAFVGLAGYGKIKEVLYALEVATKNAYSHINYTLGN
                     DVEIALAGSLNGEKGINIIAGTGSIAQALDKDGNLHRCGGWGYVLGDEGSAYYIGMAT
                     LKMFTMQSDGRCSKTKLYDLIKRHLNIENDYDIIKYVNDEIQGDRIEIAKFATICLKA
                     VIEGDNTASKIFDDAAYELSRLIIGLEHYFEQGTKIKVSYSGGVFKSGDLILESLKKY
                     LNKARFD"
     gene            complement(1010..1924)
                     /gene="murQ"
                     /locus_tag="L3M60_000872"
     CDS             complement(1010..1924)
                     /gene="murQ"
                     /locus_tag="L3M60_000872"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBN6225566.1"
                     /translation="MLIKTLENLVTEGRNKNTLQIDKEDTLGIIELINNEDKTVAYAV
                     EEQKESIAKAVNIIVDRMKQGGRLFYIGAGTSGRIGILDATECPPTYGVDFELVQAII
                     AGGNQAIFKAVEGAEDDKELGKQDIIDRGVTSKDVICGIAASGRTPYVIGAMEYAKEL
                     GCAVLSITMNPNSEMSKKADLPINIIVGAEVIMGSTRMKSGTAQKMVCNMLTTASMVK
                     MGKVYSNLMVDVKTSNEKLVERAKRIIMIATNVEYDVAEKFLEEADNSVKLAIFMIKS
                     GLDKDSAKNILDRQEGYISEALKSIERL"
     gene            complement(2023..3120)
                     /locus_tag="L3M60_000873"
     CDS             complement(2023..3120)
                     /locus_tag="L3M60_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBN6225567.1"
                     /translation="MGRLGISIYPEKTTSKEIYDYIDKVAENGFTRIFSCLLSVKDTK
                     ENIIKKFKSINEYAKFKGFEVILDVNPRVFGELEISYKDLTFFKKMKADGIRLDMGFT
                     GSEEALMTFNPQNLKIEVNMSNNTNYIDTIMDYQPNKENLIGCHNFYPHRYSGLNLEH
                     FIKSTERFNKYGLRTAVFVTSQNESTFGPWPITDGLPTIEIHRNLPIDIQVKHIIAMN
                     NINDIIISNCYPTYEELKVLGSMRKDMVTFDVAIEENIREVEKKILFEEIHFNRGDIS
                     DNLIRSTQSRVKYKGYKFELFNTPKIIKRGDIVIESSEYGHYAGELQIALTDMKNSGK
                     SNVVGYIRKEELFILDYIKPWQKFNFKPSTK"
     gene            complement(3353..4699)
                     /locus_tag="L3M60_000874"
     CDS             complement(3353..4699)
                     /locus_tag="L3M60_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6225568.1"
                     /translation="MLDKFTEFLDIKLSTSMARLAEQRHLRAVRDGIVASLPLIIVGS
                     FFLILAFPPLPDSWGVTQYIKENAVTILLPYRMTFFIISVYSTFGIGYSLAKSYKLDG
                     LTGGLLATATFLLTIVPTAIEGLTPDLLKVVEKSPELLAWYNSIPQGWQMPMANLGGG
                     SMFVAIVTSIIGVEIYRFTDKSKFKITMPEAVPASVTRSFEALTPAALIIILMAIVTY
                     FLGFDWHGFIAKLVTPLVSATDTLPSVIILIFLITFFWSFGIHGVSIIGSLARPLWIQ
                     LLDANTASYAAGQAVPGIAAEPFFQWFIWIGGSGATIGLALLLAFRAKSAYGKSLGKA
                     TVIPAIFNINEPIIFGAPIVLNPILIIPFVVAPIVMATVSWFAMSANLVSRVVVTAPW
                     TLPGPVGAYLATGGDWRASVLNLLCIAISIIIYYPFVKMYDKRLLHEEKVEEQEVY"
     gene            complement(4789..5097)
                     /locus_tag="L3M60_000875"
     CDS             complement(4789..5097)
                     /locus_tag="L3M60_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225569.1"
                     /translation="MRVLFVCSSGMSSAIAVNALQKEGTKNGIDIDVLAVGTQEFEDE
                     VKNGWDIAMVAPQVRHRFDYLKGFADEVGVPCALIKAQAYSPLGGPKLLKQVQELLSE
                     "
     gene            complement(5364..5753)
                     /locus_tag="L3M60_000876"
     CDS             complement(5364..5753)
                     /locus_tag="L3M60_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225570.1"
                     /translation="MSNNKKKKNNNYKNAKITKSNKNTKREMEKNQKTDYNIPTISFK
                     SGLILIVSSLVSTVIFPVLLSIFGIDNRLGVIIGNSLITSFAVAYTRYFIETKRGFSL
                     GFFRLYILFALSFAIIGYFWIYKGIHI"
     gene            complement(5755..6915)
                     /locus_tag="L3M60_000877"
     CDS             complement(5755..6915)
                     /locus_tag="L3M60_000877"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBN6225571.1"
                     /translation="MITMYSVGLMSGTSLDGVDAVLAEISGSGRNTKIKQIEFITLEI
                     PKDIKDEIRKCCIEEESSVDLICSLNFKLGYLFSKAVKSVCYKANFNIENLDFIASHG
                     QTIFHIPRSYNNFVSSTLQIGEPAVIAHETNTKVISNFRVMDIAAGGEGAPLVPYSEF
                     LLYSDNNKNVALQNIGGIGNITIIPKSCNIEDVFAFDTGPGNMIIDGVCQKLFNKRYD
                     KNGYFASKGKINEEMLKDLMSHKYLSQSPPKSTGREIFGQVYLDNMLNKYKHVDKYDL
                     IATVTMFTAKAIYYNYKNFILPKVNVDTLLIGGGGAHNLTLIRYIKELLLGVEVLTQD
                     EYGYSSDAKEALAFVILGNETLNNSFSNVISATGAKSKVILGNITPKPFGGK"
     gene            complement(7335..8354)
                     /locus_tag="L3M60_000878"
     CDS             complement(7335..8354)
                     /locus_tag="L3M60_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225572.1"
                     /translation="MLVKYLKGRSKYIALPLVVVSIINIYLFAIDIFKNRYSELIYLD
                     FLVLFIIMVFFVIDYINFRNSYINLYNCIEDSGEIDSYLVDGQSFEENLIKDIIENLK
                     MKNNSDIESYKQSLKELDEYIAKWVHEIKIPISSLSIITDRLSNIEDSLDIKNQIAKI
                     NFLVNSILYSSRSNTMFEDLFVNKFNLDKMVRMSIKNNSFLLIKNNVEVSLNELENDV
                     YTDSKCMSYILEQIINNAIKYSKEVGKIEFNSKKLENGVVLSIKDFGIGINEEDISRV
                     FDKGFTGKNGRNQLYKSTGMGLYFVKKMIDSLGHEIEVCSENGSYTIFNIYFYDISDY
                     LNLDS"
     gene            complement(8348..9028)
                     /locus_tag="L3M60_000879"
     CDS             complement(8348..9028)
                     /locus_tag="L3M60_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225573.1"
                     /translation="MYRILLVEDDIDLSKEIALALEKWGFKVELIDDFEIVLDEFVDK
                     KPDVVLLDVNLPLYNGFYWCEKIRAISNVPLIFLSSRDSDMDLIMGINNGADDYITKP
                     FSIDILVTKINGIIRRVYNYSDSNSILYCEDLMFDVGKGIIKHKYKDKSIELTKNEIK
                     ILTLLLKNKNRVVSRESLMMTLWDNDEFVTDNALTVNMNRLRSKVKELGFDDFIKTKK
                     GIGYIIQC"
     gene            complement(9180..11087)
                     /locus_tag="L3M60_000880"
     CDS             complement(9180..11087)
                     /locus_tag="L3M60_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6225574.1"
                     /translation="MSILGIAYNNFKSNIRTYLAFFISMVFSVVVLTNFELLRYGDAI
                     NVLQEENKKFTLSVLVAVIMILSVFLFFFIWYATNIFFKNRSKEIGILSFMGLDLYTI
                     GKIYFVENMLIGISSCITGIIIGIITSRFFQVVIIKLSGFNIKVSNGLSIEAILYATL
                     IFISIFIIMAVKGFITICRSSVINLINISKKQEKIPKVGIGIYILAIVSIIVIGYGYY
                     LSTNIRTGDITSVLPIIGIIIVGTYGLFKSVMPVIFDIIIKNKKILFNGNNIIAINNI
                     NYRLNKNYKTYAIIAIIITTTISTLGASVAMNRIHKNAQEQRNVYTISIVSMDKDEVN
                     KKQIENIIKDTNKIKYSVNPELTILKKTDIKEAYDTTDTIMKYSDFCNTLKVNGNDKE
                     LKEYGERLVSGNNVIELKSPQTIASIATKEAKIKLDGQLYNISRGEVKVPVLGTGLNQ
                     SIIVVNDDTYFKLRDKGQQVYFYGAKVFQEENSKVMFNNIEKNLSLKNGYIQNGYKAQ
                     GESQWMKFAYVVLVFLFLVFAIVAGSIIYMKIYSDAYEDKEKYKILLKIGTTEKDINR
                     AILKEVAIFYTLPMVSATISSYFALRLAGDLLMTDLFGIYILSLVICLIIFIIYGAVS
                     VNKFKSVVYKN"
     gene            complement(11088..11840)
                     /locus_tag="L3M60_000881"
     CDS             complement(11088..11840)
                     /locus_tag="L3M60_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225575.1"
                     /translation="MKEILKIKNISKDYGIKGFKTNVLKNISLTVNEGDFIAIMGPSG
                     AGKTTLLNLMSTLDKQTSGEIILDGINISKVKNNELSKLRREKIGFIFQDYNLLDNMK
                     LMDNIALPLALGKKKSKEIEAKVFSIAKKFGLENHLDKYPYQLSGGQKQRGAAARSLI
                     TNPAVIFADEPTGALDSKSAYELLESLEKINRENNATIIMITHDPLTASYSNEVYMIN
                     DGNIKCKLNKGNSRKEFYGKIMDMLASMGGEM"
     gene            complement(12158..13312)
                     /locus_tag="L3M60_000882"
     CDS             complement(12158..13312)
                     /locus_tag="L3M60_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225576.1"
                     /translation="MINKAIKFRFIIFYFLSYIIFAFASTKFTPFLSKLGYSAFERGI
                     ILSSYAITNILFQLLFGVLADKYQTMKKIVLISLSVYGIASVIMFSTESATFIIYLLL
                     VALSGGLLNTCCSLYDTWIIGCGDKINNYLSFIKTFGSIGWAIGSMVASYLILRFSYF
                     GLSVAIGLIVVLLIINILLLPDIEKINKKINVTSKDIIELLKDKKYILLVCILFLLYS
                     MVVANNCTVIDKMIALNATNSQISLKWSLQSLLEIPTYLAGSYLLKKYNSLNLLKLSA
                     IMITVQFLLFALTNDSNIIIILCVFQVFSTPLILITSKRLIFEVSPKKLRSSSQLIAL
                     SIFMGGSSLIIPTVAGFLSVNIGYNYTLMVLSLFGCLAYGLIVLLNYLKE"
     gene            complement(13315..13782)
                     /locus_tag="L3M60_000883"
     CDS             complement(13315..13782)
                     /locus_tag="L3M60_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6225577.1"
                     /translation="MFFKRKKEVITINSYIDGRIINLEEIPDKVFSTKVMGDGFAMIA
                     KDNALKSPAKGVISFIAQTNHAIGIKLQNNVQILIHIGLDSATTKKENFKLKVKQGQE
                     VNMGENLVEIDNKLLENTLYIPLVIIENPNTVKYHLSNLHQEGNSGIKVINFD"
     gene            complement(13796..14284)
                     /locus_tag="L3M60_000884"
     CDS             complement(13796..14284)
                     /locus_tag="L3M60_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225578.1"
                     /translation="MITNKIKNVTAVLKDENIFLSVIMGCFLILPVLYIVYLELTSLF
                     TNISFSELLNNDVNASLNLVSNFSAVYSAYVIYNLKKNKDIKENMIAFYLVLLGQIFQ
                     IYTLTIVIMFTYVYKFVGIKQIISSIKELQWGKSYKALFSSVAVCLISLFIMIIRIKL
                     IM"
     gene            complement(14298..15629)
                     /locus_tag="L3M60_000885"
     CDS             complement(14298..15629)
                     /locus_tag="L3M60_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBN6225579.1"
                     /translation="MERKRQRILIAGGGSTYTAGIVTMLIESVDKFPIESIKLYDNND
                     ERQRKVAEACAIIVREKNPEIKFSYTTNPEEAFTDIDFVMAQIRVGLYALREQDEKIP
                     LKYGVVGQETCGAGGVAYGLRTIGPVIEMIDYMEKYSPNAWMLNYSNPAAIVAEACRV
                     LRPNSRIINICDMPVCLEEIFARVLGLNSRKDFDVRYYGLNHFGWWTSIKDKEGNDLM
                     PKLQEYCAKKGYEEFTPQGQHKESSWLETMRAAKDLLEIEPTTLPNTYLKYYLMADET
                     VEHANPNYTRANEIMDRREKDTFEECERIIKNGTARDTWFDASEHSQFIVELACALAF
                     NTQERFLLIVPNNGAIENFADDAMVEIPCLVGKDMVEPMSIGKIPTFQKGLMEQQVAS
                     EKLAVEAWIEKSYQKLWQSFTMSKTVPSAKVAKEILDEMIVANKDFWPTFK"
     gene            complement(15643..17208)
                     /locus_tag="L3M60_000886"
     CDS             complement(15643..17208)
                     /locus_tag="L3M60_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003233606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBN6225580.1"
                     /translation="MMKKIQRFGGAMFTPTLLFAFAGIMVGFSIVFQNQSIMGSLATP
                     ENIWYQFWGVISSGAWMVFNQLPLLFAISLPIALAKKQQARACMEALATYLTFNYFVG
                     SMLSFWGKSFGVDFAAEISAGSGLVSIAGIKTLDTGMVGALLISGIVIYVHNRFYDKE
                     LPDFIGLFRGSSLVVAICFFVMIPVALLTCFIWPHVQNVIRHLQTFFINSGNIGVWCY
                     AFLQKILIPTGLHHFVYAPICYDSVVVPGGTSVYWATHIQDFQTSAKTLKEMYPIGFS
                     LSGLSKVFGSLGVFAAFYVTAKPEKKKKVLGLMIPATLTAVLTGITEPLEFTFLFIAP
                     LLFLVHAFLDACLQTISFALGVVGDFGGGIINWVVLNWLPLGMYHWKVYIIQVVVGII
                     FSFIWFFVFTFLIKKFDMKTPGREEDTEETKLYTKNEYLEAKDEKGNKLSKASQQAAE
                     YIKLVGGAENVVDVTNCATRLRLTLKDDSIISKEEDFKAVGAHGLVHNGKAIQIIIGL
                     SVPSVREEFENLL"
     gene            complement(17377..18162)
                     /locus_tag="L3M60_000887"
     CDS             complement(17377..18162)
                     /locus_tag="L3M60_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBN6225581.1"
                     /translation="MRLEEMIFLNYNKLNETDLLIWKYITANKKHCSKMSINELAMKC
                     NVSRATITRFVKKLDLKGFSEFKVLLSWESEKVHVIENQSFDIACNSIIKYVEDQKNK
                     DYDALCKLLYESRTIYAYGSGDIQNVVAKQIKRMFLSCQEIIYDFEGRTFDDSFYNIV
                     SKNDVMIIISLSGENENTLNIARRLKLIGTKIISITEFKNNTLTDLSDESIYISATNI
                     SFLQMHPSYKLTMLYFILIELLFIKYSIYKRHRMITEGIDCKL"
     gene            complement(18327..19526)
                     /locus_tag="L3M60_000888"
     CDS             complement(18327..19526)
                     /locus_tag="L3M60_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HBN6225582.1"
                     /translation="MKKKVLFLVIILAFIILLGNSTYSQKNQLSEEEKVDDFNYVYNV
                     VKTGYPYLGVNKRLNNIDWLENKDEYIKRIRSTENDEEFIEELSSILSDLNNKHTEVI
                     DNKKRYELFKKSYSNNDWYDFLNDKEVVDRYDSMNPKIKMPDDIFVKKELILKDVIKG
                     KIGYMYLPSMSSKNGSINNDLKIIGDYISTLKKHKALIIDIRGNLGGSDRYWQGIVSK
                     LIKNDVKINGYRIYRNNNEIVKKYTTARNIKLNPVESLPASVKENAPKEIIKDFNGFE
                     DTSYTIKSTDDLKFKGNIYLLVDRKIYSSSESFAMFCKETKFATLVGETTGGDGGCID
                     PVLFNLKNSGLIVRMASCMYLNKSGVCDEEFKTTPEFKVKDCKRTADFKEDNCIRKAL
                     EFENINY"
     gene            complement(20048..21385)
                     /gene="xylA"
                     /locus_tag="L3M60_000889"
     CDS             complement(20048..21385)
                     /gene="xylA"
                     /locus_tag="L3M60_000889"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBN6225583.1"
                     /translation="MNEIFKGIGQIKFEGVKSNNELAFRYYNPEQVVGNKTMKEHLRF
                     AMSYWHTLCGEGNDPFGVGTVERPWNNITDPIEIAKIKVDAGFEFMSKMGIEYFCFHD
                     RDIAPEGRDLEETNKILDEIVEYIKANMEKTGIKLLWGTANMFGNPRFVHGASTTCNA
                     DVYAYAAAQVKKAMEITKYLGGENFVFWGGREGYETLLNTNTELEMDNFARFLQMAVD
                     YAKEIGFTGQFLIEPKPKEPTKHQYDFDTATVLGFLRKYNLDKYFKVNIEANHATLAG
                     HTFQHELNIARINNVLGSIDANQGDLLLGWDTDQFPTNIYDATLAMYEVLKQGGIAPG
                     GFNFDSKVRRASFEVEDLFLAYIAGMDTFAKGLLIAHKLLEDEVFENFTKERYASFSE
                     GIGKDIVEGKVGFKELESYALQMPVIKNKSGRQEMLEAILNRYIYEVDTISNK"
     gene            complement(21489..23036)
                     /gene="xylB"
                     /locus_tag="L3M60_000890"
     CDS             complement(21489..23036)
                     /gene="xylB"
                     /locus_tag="L3M60_000890"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBN6225584.1"
                     /translation="MKLEYVLGVDIGTSGTKTVLFDKLGNTIKSCTYEYPLIQEKSGW
                     AEQDANDWWKAVVESIREVVQSSNISSECIKGIGLSGQMHGLVMLDSEGKTLRKSIIW
                     CDQRTVKECEEITDLVGEERLIEITANPALTGFTASKILWVRNNEPDIYKNTNKILLP
                     KDYIRYKLTGEYATEVSDASGMQLLDIRKRDWSDEVLEKLNIDKNLLGKVYESQEVTG
                     YVTKDVASLTGLKEGTIVVGGAGDQAAGAIGNGIVKDGVVSSTIGTSGVVFAYTKEPK
                     IDKEGRVHTFCHAIPNTWHVMGVTQGAGLSLKWFKDNFCQSEVEVSNSLDEDVYDIIN
                     NQVSQVPTGCNGLLYLPYMMGERTPHLDPYARGVFFGLSPIHSKKEIARSIMEGVSYS
                     LKDCMDIIENLNIDVNEVRASGGGGKSKVWRQMQADMFNQDVYTINSSEGPALGVAIL
                     ALVGAGIYENIQKACDTIIKTSTKLEPISENVDIYKTYHRLYKKIYKSLKDDFRLLDE
                     AVNCKNK"
     gene            23244..24404
                     /locus_tag="L3M60_000891"
     CDS             23244..24404
                     /locus_tag="L3M60_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBN6225585.1"
                     /translation="MVTDKYTIREMNERLVLEQIIKNGPISRASIASTIGLNKATISA
                     ITKKLIDESLVHEIGIGNSTHSGGRKPILLVFNKCAGISLSMDIGYDYIFSSMSYLDG
                     TIINSKKLTDIQVSKDNVIQLIDEIINSYNISKIDTPYKVIGLTLAIHGITCENKVLF
                     TPYYNLNEIDLYSILSKKYDFPIHIENEANLTALAENTFSTVHDSLLSLSIHSGFGSG
                     IIINNKLYSGRNGMSGEIGHTIIMPNGKLCPCGNRGCLEQYCSEKKVFEQLSSLENIP
                     KIDSDIVKKLYYEDNQNTKKVIHEFCSYLTIAINNAITTYAPEIIYLNSQIISDIPEI
                     LQITKDMLVSSFNKGINIEISSLGSEASLYGGSAVNIKSFLNIQNLTLINEI"
     gene            complement(24560..24964)
                     /locus_tag="L3M60_000892"
     CDS             complement(24560..24964)
                     /locus_tag="L3M60_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225586.1"
                     /translation="MNSIILMSHGNMAEEVLNSAKMIIGDSIDYPTVNMKQDDGIEGT
                     IEKLKKALEVYKESSEIIIMADLLGGTPCNAALLVANNDSRIKIISGLNLGMVIESAF
                     LDTNELVVKLEKIGKDNISMVEQNTSLLSDDE"
     gene            complement(24985..25467)
                     /locus_tag="L3M60_000893"
     CDS             complement(24985..25467)
                     /locus_tag="L3M60_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225587.1"
                     /translation="MEMKGIKNIRIDDRLIHGQVATMWSNKLGVTRLMVVNDAVANNS
                     VQKQVLRMATPAGIASSIITEETAIKNITAGKYEGQNVLLIVKSPVDLIPFVEAGFKM
                     HNINVGNMSSRKDTTVLKPNISVTEEEKEAFKRLLDEGIEITTIMTPDDKKTYLVDIL
                     "
     gene            complement(25487..26296)
                     /locus_tag="L3M60_000894"
     CDS             complement(25487..26296)
                     /locus_tag="L3M60_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBN6225588.1"
                     /translation="MSDKVSLNKSNEKVKKSVLWRWFFTSSVSSNYEKMQALAYCYAV
                     LPFLKVVYKDNPEKLQSAVLNHLQFFNTNPWVAPYILGINVAMEELSSEETEEAVASI
                     KTGLMGPVAGLGDSLFVVIPWTIFGAIAANMAIDGSPFGILLWIIVSVALKLLSIPLF
                     NAGYTSGTKLISSIEKSLKILTESTSILGLMVVGALIPSVVKANVALNFQQGDFTMKG
                     QEILDQIMPGLVPAILVVIVYWALKKNIKPIYLILGVMVVSIILYALGILK"
     gene            complement(26289..27068)
                     /locus_tag="L3M60_000895"
     CDS             complement(26289..27068)
                     /locus_tag="L3M60_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6225589.1"
                     /translation="MEPQLWQLLLIVLYGFFINYEKNSTMFGTYQPVTAGFITGLILG
                     DINTGLYIGGTLQLLSLGISNFGGASIPDYQTASIVATFITITTKQEASVGISIGIPV
                     ALLMVQLDVLRNTIGIWLVHKAEDGAKKGNYKNITYMQMLGVLLTAATTGIPVALSVI
                     FGPSLINTILKYTPEWLTGGLTVAGGLLPAVGIGLLLRYLPAKEYFSYLVIGFVLAVY
                     MKVPLLGVALIGGAIALIIYKKNLENQAQQYTVVGGMDEDE"
     gene            complement(27068..29146)
                     /locus_tag="L3M60_000896"
     CDS             complement(27068..29146)
                     /locus_tag="L3M60_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBN6225590.1"
                     /translation="MIKISEDYIEKRFDNELLRIEAWGKNSLRIRSFVDQNFVDENYA
                     LNEKPKLNKDDITINKNEDGSAIIKNGKIKAVLDHRDRITFYNDKNEILLKEYIRLRA
                     VKHDDGGEDVGTIEITKDFNSTLKLKSREYRPSYSGEFEVTTRFESEPSEKIFGMGQY
                     QHKFLDLKNTVLELAQRNSQISVPFYISSLGYGLLWNNPGIGKVSFAKNMTEWKMFST
                     KFIDYWITCGESPKELNKNYSQVTGTVPMMPENLLGLWQSKLRYRTSEEVLDVVKEYS
                     KRGIKLSSIAIDYFHWPKQGEYKFDLDYWKNPKELVKKLKEEYSVEPIVSVWPTVQSD
                     AENYNDYLENGYLVNVNRGVRMTMQIQGNTVFVDMTNENAREYVWDRIDKNYKQLGID
                     YYWLDVAEPGYSVYDFDNYRYKKGNVLSCGNIYPIDYLKMIYDGLHNDIESVVTLVRG
                     AWAGAQKYGALVWSGDIDSSFEAFNNQVNTGLNMGLAGIPWWTTDIGGFHGGNPKDPE
                     FRELMVRWFQYATFSPILRMHGDRLPHSKPLSNKGGGSMVTGAPNEIWSYGEEVEVIL
                     TKFIKIRESLKTYLKKLMKEAHEDGTPVMRTLFYEFPEDDKTWEVDNTYMLGDEILVA
                     PIMNYKDRSRKVYLPKGHTWENIFSGVSYEGGKTYEIECPLEEIPIFLKQDSSYNFKE
                     LKNILSEVKS"
     gene            complement(29754..29933)
                     /locus_tag="L3M60_000897"
     CDS             complement(29754..29933)
                     /locus_tag="L3M60_000897"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225591.1"
                     /translation="MVLSMTGYVANISQTSQALCGIRSINGIIPIVLTVAMLVQMYSL
                     AERKCREVINELRSK"
     gene            complement(30092..30613)
                     /locus_tag="L3M60_000898"
     CDS             complement(30092..30613)
                     /locus_tag="L3M60_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBN6225592.1"
                     /translation="MKRNREIVVVCHCILNCNSKVEGLSEFKGAQNIAIDLINKGYGI
                     IQLPCPEMIMYGINRWGHVKEQFDNLFFREQCRQMLIPYVRQFGEYLKNNYDIKSIIA
                     IDGSPSCGYNKTCSSKRWFGELSGCSELMDKINDIQMVEGKGIFIEELEKLLKEYNLE
                     IPIVGFDEIREGK"
     gene            30779..31345
                     /locus_tag="L3M60_000899"
     CDS             30779..31345
                     /locus_tag="L3M60_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBN6225593.1"
                     /translation="MDTKKVTFSSMCIVINIVLGMVISMINIPLLFLDTVGTVLGAVV
                     LGPFWGALIGGCTNLVLGVISGPTNIPFALVNIVLGLIVGYISNKKGFGYKEAIITGV
                     ILSIVCPLIGTPISVLLFGGLSGSGADLLVGFLVQSGQKIFTAAFFPRILSNIVDKPL
                     SCIMVVYFISKMPKGFIKQYSKNTYSHS"
     gene            complement(31616..31789)
                     /locus_tag="L3M60_000900"
     CDS             complement(31616..31789)
                     /locus_tag="L3M60_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HBN6225594.1"
                     /translation="MSNEKYIIKNILLKTLRKLRNNNSYGYDISEFKDMYELFIENED
                     VFVSIRKLAKYDF"
     gene            complement(31866..32735)
                     /locus_tag="L3M60_000901"
     CDS             complement(31866..32735)
                     /locus_tag="L3M60_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBN6225595.1"
                     /translation="MALVTTKQILLDAQEGHYAVGAFNVENMEMVMAVIEAAEELKSP
                     VILQTTPSTIKYAGLDYYLANVKVASEKASVPVAMHLDHGSSFGLAMQALRAGYTSIM
                     IDGSHESYEDNISISKSVVDACMPSDIPVEAELGKVGGKEDELDGGDGGAYTDPLEAK
                     EFVERTGVSSLAVAIGTAHGLYKGEPKLDLDRLSEIREVVSVPLVLHGGSGIPDEVIK
                     ESIKRGICKVNYATELRIAYSNGVKDVLNSDPEVIDPKKYGKKGLESVKDFVKSRMEV
                     CGCVGKAIETVKC"
     gene            complement(32755..34671)
                     /locus_tag="L3M60_000902"
     CDS             complement(32755..34671)
                     /locus_tag="L3M60_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HBN6225596.1"
                     /translation="MGKKMILGVTGCPTGIAHTFMAEEALKNSARELGCDIKVETNGA
                     IGVENKLTAKDIEMADAIIVACDKNVDMDRFNGKPVIEVPVKEGIHKADELIQRCIDG
                     KVAIRKGNSSSSKIAEEGNLSFGQKLYKDLMNGVSHMLPLVVAGGVLTAISFLWGIYS
                     FDPNSEQYNQIAATLKSVGGYSMNLMVPVLAAFIAQSISGRAGMLAGLVGGMISFDTG
                     SGFLGGIISGFLAGYLVKLFVHLLRKLPRQLEGLKSIFIIPIVSVGLVGVAMLLLGGP
                     CSALNNAMMNFLSGLQNSSPIILGIVIGCMSAFDMGGPVNKAAYVTGTMLLGQGNYLF
                     MAGVSAACITPPLVIAIASTLFKNRFTEEDRAAGLINYILGSTHITEGAIPFAAKNPL
                     KVLPVLMIASSISSILTYIMKIEVPAPHGGFLVLGLVNKPLLWVGCIFVGSLVGAVLY
                     MMVTPKVVNEKNTIENTNATKDERKNKSAESENVSNNISLYSEETVILDVKGKNKTDV
                     IDEMVGVLDKSGVLLDKNKFKEEILKREEISSTGFGMGIAIPHAKTDAVKIPRVAVGV
                     SKEGFDFESEDGSLVHIIFMIAATDNGDNLHLKTLSQLSAKLMNEEFLNELLNSKTSR
                     EIVSKLNNEEIKSV"
     gene            complement(34674..35594)
                     /locus_tag="L3M60_000903"
     CDS             complement(34674..35594)
                     /locus_tag="L3M60_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HBN6225597.1"
                     /translation="MIYTLTTNPAIDMNISTNGIKRKLVNRTSNAVYSPNGKGLNVTF
                     VLGHYGIESKILGFFGGFSGKYIVEESEKRGFNVLPTWVEDTTRINIFLNDGSDEFKF
                     VNSGSYVNEKQKLDMLEKIQSLEDISYLSISGSLPPGIDDNYYEDIFKICKNKSIKTI
                     LDISSPKLKELLQYNPYLIKPNDEEIKDIFGIIVRDEEDIKDVLKLLHLKGAQNILLT
                     LGEKGSYFYNGESIYYASAQPVTVVSSACAGDSALAAFLSIWLENPEHIEEALKRSAA
                     TGASVAESNGIGNLENVEDYIKNIRVRKVV"
     gene            complement(35696..36448)
                     /locus_tag="L3M60_000904"
     CDS             complement(35696..36448)
                     /locus_tag="L3M60_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBN6225598.1"
                     /translation="MIIELNKSNSENLTKTELEIIKFINENESILPELSIVEIAHETY
                     SSPATVSRAIRKCGLNGFNELRYRLTVKDENDDIQNMGDIINKSFIEAQRVIEQISLT
                     TLINIIKTINQSSKIYILARGLTEYVAKEFSLKLQLLDFNVVFINDPNIMKIKSKSIK
                     KDELIIIFSLNGNTIELVESAQNANLVGAKVITCCCSESAKLIKYSDLYIVGYKHEHI
                     SISEYEVSSRLPLYIISRIIIDYMVRYNNSEN"
BASE COUNT        11911 a         6576 c         3853 g        14187 t
ORIGIN      
        1 tttagctact aactcatgac taaagtcaca agtatgcgta gctatttttt aatcaaatct
       61 cgctttattc aaatattttt taagtgattc taatatcaaa tctcctgact taaacactcc
      121 acctgaataa gataccttta tctttgtacc ttgttcaaaa taatgctcta atcctattat
      181 aagcctactt aattcataag ctgcatcatc aaatatttta ctagcagtat tatcaccttc
      241 aataactgct tttaaacata tcgtagcaaa ctttgctatt tctattctat ctccttgtat
      301 ttcatcattt acatatttta ttatgtcata atcattctct atattaagat gtctttttat
      361 taaatcatat aattttgttt ttgaacacct tccatcagac tgcattgtga acatttttag
      421 ggtagccatt cctatataat atgctgaccc ttcatctcct aatacatagc cccatcctcc
      481 acatctgtga agattcccat ctttatctaa agcctgtgct attgaaccag tacctgcaat
      541 tatatttata cctttttccc catttaaaga acctgctaat gctatttcta catcattacc
      601 taaagtataa tttatatggc tatatgcatt ttttgttgct acttctaatg catacaatac
      661 ttcttttatt tttccatatc cagcaagtcc aacaaatgca tacgttattt cttctacatt
      721 tatcttagca tctttacaaa cttcttccaa cccagttatt aataatttcg ttagattatc
      781 aaatcctatt tgattataat gacaagttcc tttggttatg gttcctacta tatttaattc
      841 ttcatctact aatgtaaatg ttgttttcgt tcctccacca tctaccccta aaaaatacat
      901 actatcctcc tacaatattt ttaataattc tttcaatact taatatctaa aaataacctt
      961 ccaatgcagt tcttttaata ttgaaaggtt atcagtatta ttatttattt tacaatcttt
     1021 ctattgattt taaagcttca cttatataac cttcttgtct atctaggata ttctttgcac
     1081 tatccttatc taaaccactt tttatcataa atatagccaa tttaacgcta ttatctgctt
     1141 cttctaagaa tttttctgca acgtcatatt ctacatttgt agctatcatt attattcttt
     1201 ttgctctttc aaccaacttt tcatttgatg tcttaacatc taccatcaag tttgagtata
     1261 cttttcccat cttaaccata gaagctgttg tcaacatatt acataccatt ttttgagctg
     1321 taccactttt cattctagtt gaacccatta ttacttctgc ccctactata atgtttattg
     1381 gtaaatccgc ttttttactc atttcactat tcggattcat agttattgat agtactgcac
     1441 accctaattc cttagcatac tccattgcac ctatcacata aggagtcctt cctgatgcag
     1501 ctataccaca tataacatcc ttagatgtaa ctcctctatc tattatatct tgttttccaa
     1561 gttctttatc atcttcagca ccttctactg ctttaaatat tgcttgattt ccaccagcta
     1621 ttatagcttg aacaagctca aaatccactc cgtaagttgg aggacattct gtggcatcta
     1681 gtatccctat tctcccactg gtacctgctc ctatatagaa taatctacct ccctgcttca
     1741 ttctatctac tattatatta acagcttttg ctatactctc cttttgttct tcaacagcat
     1801 aggctacagt tttatcttcg ttatttatta attctattat gcctaatgta tcttctttat
     1861 ctatttgcaa tgtattctta tttctacctt ctgtaactaa gttttctaaa gtttttatta
     1921 acatatcatt tcctcatttt cttaatatta aatttttaaa cacattttaa ttccaacctt
     1981 catgattata tctacataat aaattaatat tttcataata aattattttg ttgaaggctt
     2041 aaaattaaac ttttgccatg gtttaatgta atctaatata aataattctt cttttcttat
     2101 atatcctact acattcgatt taccactgtt tttcatatca gtcaaagcta tttgtaactc
     2161 ccccgcataa tgaccatatt cactactttc aattactata tctcctcttt ttattatttt
     2221 aggagtatta aatagttcga atttgtatcc tttatattta acacgagatt gagtagacct
     2281 tatcagatta tcagatatat cccctctatt aaaatgtatt tcttcaaaga gtatcttctt
     2341 ctcaacttct cgtatatttt cctctattgc tacatcaaat gtaaccatat cttttctcat
     2401 agaacctaat accttaagct cctcatatgt tggataacaa tttgatatta ttatatcatt
     2461 tatattattc atagctatta tatgctttac ttgaatatct ataggtagat ttctgtgaat
     2521 ctcaattgtt ggaagtccat cagttatagg ccatggtcca aatgtacttt cattttgact
     2581 tgttacaaaa actgctgttc ttaatccata cttattaaat ctttctgtag attttataaa
     2641 gtgttctaaa tttaatccac tatatctgtg tggataaaag ttatgacatc ctatcaaatt
     2701 ctctttgttt ggttggtagt ccattattgt atctatataa ttagtattgt tactcatatt
     2761 gacttctatt ttaaggttct gaggattaaa agtcattaat gcctcttcag accctgtaaa
     2821 tcccatatca agccttattc catctgcctt catcttttta aaaaaagtca aatctttgta
     2881 gcttatttct aattcaccaa atactctggg atttacatca agtattacct caaaaccttt
     2941 aaacttagcg tattcgttta tacttttaaa ttttttaatt atattttctt tagtatcttt
     3001 aactgataat aaacatgaaa atatacgtgt aaatccattt tcagcaacct tatctatata
     3061 atcatatatt tccttagatg ttgttttttc tggataaatt gatattccta atcttcccat
     3121 ctgtgtcatc tccttatatc ctatatgtgt ttaaatttat ttagaattac acgaaacttt
     3181 tttaaaaatt tggtgaaaaa aagtttcata tatatttata aaaaaagaaa agtttacctt
     3241 ttcttttttt ataaacaatc aacaaaatat ctttgttact tatttcataa ctgttatact
     3301 ttgaaattta tactaatcta atatagtttt taattttcaa tatagtttct aattaataaa
     3361 cttcttgctc ttcaactttt tcttcatgaa gcaacctctt atcgtacatc ttgacaaatg
     3421 gatagtaaat tattattgat atagctatac ataataagtt gagaactgaa gctctccaat
     3481 ctccaccagt ggctaaatat gcccctactg gtcctggtaa tgtccaagga gcagtaacta
     3541 ctactctaga tactaaatta gcactcatag caaaccacga aactgttgcc attactattg
     3601 gtgctacaac aaatggtatt attaatattg ggtttaatac tattggtgct ccaaatatta
     3661 ttggttcatt tatattgaat attgctggta ttacagttgc ttttcctaaa gactttccat
     3721 aagctgattt tgctctaaat gcaagaagta aagctaagcc tattgtagct ccagaacctc
     3781 caatccatat aaaccattga aaaaatggtt cagcagcaat acctggaact gcttgacctg
     3841 cagcatatga agctgtatta gcatctaata attgtatcca taaaggtctt gccaatgaac
     3901 ctattattga tacaccatgt attccaaatg accaaaaaaa cgttattaaa aatattagta
     3961 taattactga tggtaatgtg tcagtggctg aaactaatgg agttactagt ttagctatga
     4021 aaccatgcca atcaaatcct aggaaataag taacaatagc cattaaaatt attattaatg
     4081 cagctggtgt cagagcttca aaagaccttg tgactgatgc tggtactgct tctggcatgg
     4141 ttatcttgaa cttagatttg tcagtaaatc tataaatctc aacacctatt atagacgtta
     4201 caatagcaac aaacattgaa cctccaccaa gattagccat tggcatttgc catccttgag
     4261 gaattgaatt ataccaagct aaaagttctg gactcttttc aactaccttt aataaatcag
     4321 gggtaagacc ctctattgca gtaggaacta ttgtcaaaag gaatgtagct gtagctaata
     4381 aacctcctgt caatccatct aacttatatg actttgccaa actataacct atgccaaatg
     4441 tagaatatac tgatataata aaaaatgtca tcctatatgg tagtaatatt gtaactgcat
     4501 tttcctttat atactgagtt accccccatg aatctggtaa tggaggaaat gctaatatca
     4561 agaaaaatga accaactatt ataagtggta gtgaagctac gattccatct cttacagctc
     4621 ttaagtgacg ttgttctgcc aatctagcca tagatgttga taatttaatg tctaaaaatt
     4681 ctgtaaattt atctagcatc ttttctctcc ttaattaata tttattttta ttatcattaa
     4741 aatttttgta aaattaactt tcttctactc tagcaacttc taaaactgct attcactcaa
     4801 cagctcttga acttgtttta ataactttgg ccctcctaat ggactataag cttgtgcttt
     4861 tattaatgca catggtacac caacttcatc agcgaaacct tttaaatagt caaatctatg
     4921 tctgacttgt ggagctacca tagcaatatc ccatccattt ttaacttcat cctcaaactc
     4981 ttgagtgcca acagctaaaa catctatatc tattccattt tttgttcctt ctttttgtaa
     5041 tgcattaact gctatagcac ttgacatacc tgatgaacaa acaaataaca ctctcatatt
     5101 tattccccct tattttttta tttcttttca atcattatag catgtatttt taattacagg
     5161 aatacatttt cacgcttttt ttcattattt acaaaattac ttgtaatttt atttcaaata
     5221 tttatatctt attttaatct atttatatac ctaatcattc tttttactaa aattacacac
     5281 aacttcaaat acttatatct tcttttacaa aaaaaagcat ggagactatc tcattattac
     5341 cataaaatag tctccatact tttttaaata tgtatacctt tatatatcca gaagtaacct
     5401 attattgcaa atgacaaagc aaataaaata tacagtctaa aaaatcctaa agaaaaacct
     5461 ctcttagttt caataaagta ccttgtatat gcaactgcaa aagatgtaat taatgaattt
     5521 cctataataa ctcctaatct attatctatt ccaaatatag ataataaaac aggaaaaatt
     5581 acagtactta ctagagatga cactattagt attaatccac ttttaaaact tatagttgga
     5641 atattataat ctgttttttg atttttctcc atttctctct tagtattttt attacttttt
     5701 gtaatttttg catttttata attattattt ttcttttttt tattattaga catgtcattt
     5761 acctccaaaa ggtttaggtg ttatattccc taaaataact ttacttttag cccctgttgc
     5821 agatattaca ttagaaaaag aattatttaa tgtttcattg cccaagatta caaatgccaa
     5881 agcctccttt gcatcagaag aatacccata ttcatcctga gttaatactt caacccctaa
     5941 taataactct tttatatatc ttattaaagt tagattatga gctcctccac ctccaattaa
     6001 taaagtatct acattaacct ttggaagtat gaagttttta taattataat atatagcttt
     6061 tgctgtaaac atagttactg ttgctatcaa atcatattta tctacatgtt tatatttgtt
     6121 aagcatgtta tctaaataga cctgtccaaa tatctctctt ccagtgcttt ttggtggtga
     6181 ttgactaaga tatttatgac tcatcaaatc ttttaacatt tcttcattaa ttttaccttt
     6241 tgatgcaaag tatccatttt tatcatacct tttattaaat aatttttgac atactccatc
     6301 tattatcata tttcctggac ctgtatcaaa tgcaaagaca tcctctatgt tacatgattt
     6361 aggtattata gtaatatttc ctattccacc aatattttga agtgctacat ttttattgtt
     6421 atcactatat aataaaaatt cactatatgg gactaatgga gcaccttctc cacctgctgc
     6481 tatatccatc actctaaaat tagatattac ttttgtattt gtttcatgtg ctataactgc
     6541 tggttcacct atttgaagcg ttgaagatac aaaattatta taacttcttg gtatatgaaa
     6601 gattgtttga ccatgagatg cgataaaatc caaattttct atattaaagt ttgctttata
     6661 acatactgat tttacagctt tactaaataa atatccaagc ttaaaattta agctacaaat
     6721 taaatcaaca ctcgattcct cttctataca gcactttctt atttcgtctt ttatatcttt
     6781 aggaatttct agagttataa attctatttg cttaattttg gtatttctac cactcccact
     6841 aatttcagct agtactgcat caactccgtc taaggaagtt cctgacatta aacctacact
     6901 atacatagtt atcatccttt taagtttata gttattcctc cccaatcaaa gtattaagtt
     6961 taataatttt agaattaata ttattccatc ttgaattatg ataatttact cttacgaaat
     7021 taattgagtt aatcttatat acggttttta ccttagctaa atcttacctc aatttatttt
     7081 cagtgtcaat gtaatctcat gtaatttttt ttcacataca aaacacattc tatctccaaa
     7141 aaacatatta aaatagttgc tacaaataaa aaacatacca ggtatctatc tggtatgttt
     7201 ttcatatata ataattatta ttttataaga tggatagagc atcatttaag tcactaatta
     7261 aatcttcact attttcaaga actaaactta ttcttattct ctatggcgat atattttata
     7321 gcctttataa taacctaact atctaggttt aaataatcag atatatcata aaaataaata
     7381 ttaaaaattg tatatgaacc attttccgaa catacctcaa tctcatgccc taaactatct
     7441 atcattttct ttacaaaata cagccccatt ccagtagact tatacaattg atttcttcca
     7501 tttttccccg taaatccctt gtcaaaaact ctagatatat cttcttcatt tatacctata
     7561 ccaaaatctt ttatagataa taccactcca ttttctaatt tttttgaatt aaattcaatc
     7621 ttaccaactt cctttgaata ctttatagca ttattaataa tttgttctaa aatatatgac
     7681 atgcatttag aatctgtata aacatcattt tctaattcat tcaaactaac ttctacatta
     7741 ttttttataa gcaaaaatga attatttttt atagacattc tcaccatttt atctaaatta
     7801 aatttattta caaataaatc ttcaaacata gtgttacttc tactactata caagatagag
     7861 tttactaaga aatttatctt tgcaatctga ttcttaatat ccaaactatc ttctatgtta
     7921 cttaacctat ctgtaattat acttaaagaa gatattggaa tttttatttc atgaacccat
     7981 ttagctatgt attcatctaa ttcttttaaa gactgtttat aactctctat gtcactatta
     8041 tttttcattt ttaaattttc tataatatct tttattagat tttcttcaaa agattgtcca
     8101 tcaactaaat aactgtctat ttcaccacta tcttctatac aattatataa atttatataa
     8161 ctatttctaa aatttatata atctataaca aaaaatacca ttattataaa cagcactaaa
     8221 aaatctaagt atattaactc tgaatatcta tttttaaaaa tatctatagc aaataaatag
     8281 atatttatta tactaactac aactaaaggt agagcaatat acttagacct accttttaaa
     8341 tattttacta gcattgtatt atatatccta ttcccttctt agtttttata aaatcatcaa
     8401 aacctaattc tttaacttta cttctcaatc tattcatatt tacagtaagt gcattgtctg
     8461 tcacaaactc atcattatcc catagagtca tcataagact ttcccttgat acaaccctat
     8521 tcttattttt taataataat gttaaaattt ttatttcgtt tttagtcaac tctatacttt
     8581 tatccttata cttatgtttt ataatccctt tccccacatc aaacatcaaa tcttcacaat
     8641 acaaaatact attagaatca ctataattat agactcttct tattatacca tttattttgg
     8701 taaccaatat gtctatagaa aatggtttgg taatataatc atctgctcca ttatttatac
     8761 ccattattaa atccatatca gaatcccttg atgataaaaa tattaatggt acatttgaaa
     8821 tagctcttat tttttcgcac caataaaatc cattatataa tggtaaattt acatctaaaa
     8881 gtactacatc tggtttttta tccacaaatt catctaatac tatctcaaaa tcatcaatta
     8941 attctacttt aaatccccat ttctcaagtg ctaacgcaat ctcttttgat aaatcaatat
     9001 cgtcctctac taataaaatt ctatacattt gtatattctc cattatccta atatttcact
     9061 atgccacgaa ttattaatat cataacatat tttatataaa tttattaccc aaatcattta
     9121 gttaatcaac aaggagactc ttattacaag aatctccttt ctctttatta atatataaac
     9181 taatttttat acactacact cttaaactta ttaacagata ctgctccata tattatgaat
     9241 attatcaaac atatcactaa acttagtata tatataccaa ataagtcggt catcaataaa
     9301 tctcctgcta atctcaatgc aaaataacta gatatagttg cacttaccat tggcaaagta
     9361 taaaatatag ctacttcttt aagtatagct ctatttatat ctttttctgt agtacctatt
     9421 tttaataaaa tcttgtattt ttctttatct tcatatgcat cactatatat tttcatatat
     9481 attatacttc cagctacaat tgcaaagact aagaataaaa acacaagcac tacatatgca
     9541 aacttcatcc attgactctc gccttgagct ttatatccat tctgaatata tccatttttt
     9601 aaacttaagt tcttttcaat attgttgaac attactttgc tattttcttc ttgaaatacc
     9661 tttgcaccat aaaaatatac ttgttgaccc ttatctctta atttgaaata tgtatcgtca
     9721 tttactacta tgatactttg attaagtcct gtccctagta ctggaacttt tacttctccc
     9781 cttgaaatat tatataactg accatcaagt tttatcttgg cttcttttgt agctattgat
     9841 gctatcgttt gagggctttt taactctatt acattatttc cactaactaa tctttcacca
     9901 tactctttta attctttatc attgccattc actttaagag tattacaaaa atcactatat
     9961 ttcattatag tatctgttgt atcataagct tcttttatat ctgttttctt taaaattgtt
    10021 agttctgggt ttactgaata ctttatctta ttagtatctt ttattatatt ctctatttgt
    10081 tttttattca cttcatcttt atccattgaa actatgctaa tagtgtacac atttctttgt
    10141 tcttgggcat tcttatgtat acgattcatc gcaactgaag caccaagagt tgatattgtt
    10201 gtagttataa ttatagctat tatagcataa gttttatagt ttttatttaa tcggtagttt
    10261 atattgttta ttgcaattat attatttcca ttaaataata tttttttatt tttgattata
    10321 atatcaaata taacaggcat aactgatttg aataagccat aagtacctac tattattata
    10381 cctataatag gtagtacact agttatatct ccagttctta tattagtaga aagatagtag
    10441 ccatatccta ttactataat tgatactata gctaatatat atatacctat tcctaccttt
    10501 ggtatttttt cttgtttttt agatatattg attagattta ttacactact tctacatatt
    10561 gttataaaac cttttacagc cattattata aatatagaaa taaatattaa agtagcatat
    10621 agtatagctt ctatgctaag tccattagat acctttatat taaatccaga taactttatt
    10681 atcactactt gaaaaaatct agatgttatt atccctataa ttattcctgt tatacaagaa
    10741 cttattccta taagcatatt ttctacaaaa tatatttttc ctatagtata taaatctaat
    10801 cccataaagg acaatattcc aatttcttta gacctattct taaaaaatat atttgttgca
    10861 taccatataa agaaaaataa aaatactgat agtatcatta ttacagctac taacactgat
    10921 agtgtaaatt ttttattttc ctcttgtaac acatttatag catcaccata tctcaataat
    10981 tcaaaattag ttagcacaac tactgaaaaa accatagata taaaaaatgc tagataagtt
    11041 cttatgttac tcttaaaatt attataagca attccaagta tactcactta catctcacct
    11101 cccatagagg ctagcatatc cataatcttt ccataaaatt cttttctact atttcctttg
    11161 tttaatttac actttatatt tccatcattt atcatatata cttcatttga atagcttgcc
    11221 gttagagggt catgtgtaat cattataata gtagcgttgt tttctctgtt tattttttct
    11281 aatgattcta aaagttcata tgctgattta gagtctaagg cacctgttgg ttcatcagca
    11341 aatatgactg ctggatttgt aattaatgac ctagctgctg cgcctctttg tttttgtcct
    11401 cctgataact gatatggata tttatctaaa tgattttcta atccaaattt tttggctata
    11461 gaaaatacct tagcttctat ctccttactt ttcttttttc ctaatgctag aggaagtgct
    11521 atattgtcca ttagtttcat attgtctaat agattatagt cttgaaatat aaaccctatt
    11581 ttttctcttc ttagttttga tagttcatta ttttttactt ttgatatatt aattccatct
    11641 agtattattt ctcctgaagt ttgtttgtca agtgtagaca ttaaatttag taatgttgtc
    11701 tttcctgcac cagatggacc cattatcgct ataaaatcac cttcattgac tgtaagagat
    11761 atatttttta acacatttgt tttaaatcct tttattccat aatcttttga gatatttttt
    11821 atttttaata tttctttcat aataatagcc tccagttgct aattttttaa tatatcttta
    11881 ttttaaaggt tttatataaa taaatcctta agtaaatatt acattttaac tgttagcatt
    11941 acacttttgt aacatatgga agactattat agttatagtg gaaatatctt aaaataaaag
    12001 catgattcac ttttttgtat aaacaaagaa aagaagcccc taagaagcct ctaatctata
    12061 caataattct tctctataaa aaaccaaaaa tacaaccttg attgtggtaa atacaggtac
    12121 tataaacaat tgatattacc actatatatt aaaacaatta ttccttaaga taattcagta
    12181 agactatcaa gccataagct aagcatccaa aaagacttaa taccattaat gtataattat
    12241 aaccaatatt aacgctcaaa aaaccagcaa cagttggaat aatcagagat gaccccccca
    12301 taaatatact taaagctatt aattggctac tacttctaag ttttttagga gaaacttcaa
    12361 atattaatct ttttgaagta attaaaatta aaggagtaga aaacacttga aaaacacaca
    12421 gtataataat gatatttgaa tcatttgtta aagcaaataa tagaaactga actgttatca
    12481 taatagcact taattttaat aagtttaaac tgttatattt ttttaataaa taagaaccag
    12541 ctaaatatgt tggtatctct aataatgact gtaaagacca ctttaaagat atttgagaat
    12601 ttgtagcatt taaagctatc attttatcta ttacagtaca gttatttgct accaccattg
    12661 aatatagtaa aaataaaata catactagta agatatattt tttatctttt aataattcaa
    12721 taatatcttt actggttaca tttatttttt tgttaatctt ttcaatatca ggtagtaaaa
    12781 gtatatttat aataagtaat actacaatta aaccaatagc tacagataat ccaaaatacg
    12841 agaatctaag aatcaagtaa gatgccacca tacttccaat agcccatcca attgacccaa
    12901 aagtcttaat aaatgacaaa taattattaa ttttatctcc acaacctatt atccatgtat
    12961 catataaact acagcaagta tttaataatc ctcctgacaa agcaacaagt aataagtata
    13021 ttataaaagt tgcagattca gtagaaaaca taataactga tgcaattcca tatactgata
    13081 gactaattaa aacaattttt ttcattgttt gatacttatc agctaatact ccaaataata
    13141 attgaaataa tatattggtt attgcatagc ttgataatat aattcctctt tcaaatgctg
    13201 agtaacctaa ttttgataaa aaaggtgtga attttgttga tgcaaaggca aaaataatat
    13261 atgagagaaa ataaaatatg ataaatctaa atttaatcgc tttattaatc ataatcaatc
    13321 aaaattgata actttaatac cactatttcc ttcctgatgt aagtttgaaa gatgatattt
    13381 taccgtatta ggattctcta taataactaa aggaatatat aaagtatttt caagtaattt
    13441 attatcaatt tctactagat tttcccccat attaacttct tgaccttgtt taacttttaa
    13501 tttaaagttt tctttttttg ttgttgcact atccagtcca atatgaataa gaatttgaac
    13561 attattttgt aatttgattc ctatagcatg gttagtttga gcaataaaac ttattactcc
    13621 tttagcaggt gatttaagag cattgtcctt tgctatcata gcaaatccat cccccattac
    13681 cttagttgaa aatactttat ctggaatttc ttcaagatta attatccttc catcaatata
    13741 ggaattaatt gtaattactt ctttttttct tttaaagaac actataaaca ctcctctaca
    13801 taattagctt tattctgatt atcattataa ataacgatat taagcatact gcaactgagg
    13861 aaaaaagagc cttatagctc tttccccatt gtagttcttt aatactgctt atgatttgtt
    13921 taattccaac aaatttataa acatatgtaa acatgataac aatagttagt gtgtaaattt
    13981 ggaatatctg ccctaataag actaaataga aagcaatcat gttttctttt atatctttat
    14041 tcttttttaa attataaata acatatgcac tatatactgc actaaaatta gagactaaat
    14101 ttaaacttgc attaacatca ttatttaaaa gttctgaaaa tgaaatattt gtaaataatg
    14161 atgtcaattc taaatacact atatataaaa caggcaatat tagaaaacaa cccattataa
    14221 ctgaaagaaa tatattttca tcttttaata ctgctgtgac attttttatt ttatttgtta
    14281 tcatgttttt attctcctta tttaaaagtt ggccagaaat ccttattagc cacaatcatt
    14341 tcatctaaga tttcttttgc tacttttgca cttggaacag ttttactcat agtaaacgac
    14401 tgccataatt tttgataact tttttctatc cacgcttcta cagctaattt ttcagaagca
    14461 acttgttgtt ccattagacc tttttggaaa gttggaattt tacctataga cataggctca
    14521 accatatctt ttccaacaag acaaggaatt tcaaccattg catcatctgc aaaattttca
    14581 atagctccat tattaggaac aatcagtaaa aatctttcct gagtattgaa tgctaatgca
    14641 caagctaatt caacaataaa ttgagaatgt tcacttgcat caaaccatgt atctcttgct
    14701 gtaccatttt ttataattct ttcacattct tcgaatgtat ccttttctct tctatccata
    14761 atttcatttg ctcttgtata atttggattg gcatgttcaa cagtttcatc agccattaaa
    14821 taatatttta aataagtatt aggtaatgta gttggttcta tttctaacaa atcctttgcc
    14881 gctctcatag tttctagcca actagattct ttatgttgac cttgaggtgt aaattcctca
    14941 tatccttttt tagcacaata ttcctgtaat ttaggcatta aatcatttcc ttctttgtcc
    15001 ttaatacttg tccaccatcc aaaatgattc aatccataat atctaacatc aaaatcttta
    15061 cgagaattta aacctaaaac tctagcaaat atttcctcta gacatactgg catatcacaa
    15121 atattaataa ttcttgaatt tggtcttaat acacgacatg cttctgcaac tatagctgct
    15181 ggatttgagt aattaagcat ccaagcattt ggagaatatt tttccatata gtcaatcatt
    15241 tctatcactg gacctattgt tcttaatcca taggcaactc cacctgctcc acaagtttct
    15301 tgaccaacaa ccccatattt tagtggaatt ttttcatctt gttcacgtaa agcatataaa
    15361 ccaacacgaa tttgtgccat tacaaaatca atatcagtaa atgcttcttc tggatttgtt
    15421 gtataactaa atttaatttc tggatttttc tctctaacaa ttattgcaca tgcttcagca
    15481 acttttcttt gtctctcatc attattatca tataatttaa ttgattcaat aggaaattta
    15541 tcaacacttt caattaacat tgttacaatt ccagcagtat aagtactacc tccaccagcg
    15601 attagtatcc tctgtctttt tctttccatc tttccttctc ctttacaata aattttcaaa
    15661 ttcttctctt acacttggta ctgaaagtcc aataataatc tgaatagctt ttccattatg
    15721 cactaaacca tgtgcaccta ctgccttaaa atcttcttct ttagaaatga ttgagtcatc
    15781 tttcaaggtt aatctcagcc ttgtagcaca gtttgtaaca tctacaacat tttctgctcc
    15841 accaactaat tttatatatt cagctgcttg ttgagatgct ttacttaatt tatttccttt
    15901 ttcatcttta gcttctaaat actcattttt ggtatatagt tttgtttctt cagtatcttc
    15961 ctctctacca ggagttttca tatcaaactt cttaattaag aaagtaaata caaagaacca
    16021 gataaacgaa aagataatcc ctacaacaac ttgaataata tataccttcc agtgatacat
    16081 tccaagagga agccaattta atacaaccca gttaataatt cctccaccaa aatcaccaac
    16141 aactcctaag gcaaacgaaa tcgtttgcaa gcatgcatct aaaaatgcat gtactaggaa
    16201 taaaagtggt gctataaata aaaatgtaaa ttctaacggc tcagtaattc cagttaatac
    16261 tgcagtaaga gtagcaggaa tcattaaacc taacactttc ttcttttttt ctggctttgc
    16321 agttacatag aaagcagcaa atactcctaa tgaaccaaac accttcgata gcccagataa
    16381 tgaaaatcca attggataca tttcctttag agtcttagct gaagtttgaa agtcctgaat
    16441 atgagtagcc caatatactg atgtaccacc tggaacaaca acactatcat aacaaattgg
    16501 tgcatataca aaatgatgta atccagttgg tattaaaatc ttttgtaaga acgcataaca
    16561 ccaaacgcct atatttccac tatttataaa aaacgtttgc aaatgtctaa taacattttg
    16621 aacatgaggc caaataaagc aagttaataa tgcaactggt atcataacaa agaaacaaat
    16681 tgcaactaca agtgatgaac ctctaaataa tccaataaaa tctggtagtt ctttatcata
    16741 aaatctatta tgaacataaa taacaatacc tgatattaac aaagcaccaa ccataccagt
    16801 atctagtgtt ttaataccag caatgcttac aagtccactt ccagcactta tttcagctgc
    16861 gaaatcaact ccaaagcttt ttccccaaaa acttaacata gaacctacaa aataattaaa
    16921 agtcaagtat gttgctaaag cttccataca agctcttgct tgttgctttt ttgcaagtgc
    16981 tatgggtaaa gaaatagcaa ataataatgg taattgattg aataccatcc aagctccact
    17041 agatataaca ccccaaaatt ggtaccaaat attttctggt gtagcaagtg aacccataat
    17101 tgactgattt tgaaatacaa ttgagaatcc aaccattatc ccagcaaaag caaataatag
    17161 tgttggtgta aacattgccc ctccaaaacg ttgtattttc ttcatcatat aaagtactcc
    17221 cctcctcata tttttatttt atctttggcc ttagatatct attattttaa catgttaata
    17281 aatatataca atctttggac tacattcgga gcttattcac agtcaaataa gtttttgcac
    17341 atttttatgt tcaaattttc aaaaaaaaaa gaatgactac aatttgcaat caatcccttc
    17401 tgtaatcatt ctatgccttt tataaatact atatttaata aataacaact caatcaatat
    17461 aaaatataac attgtcaatt tataactagg gtgcatttgt aaaaatgaga tattagtcgc
    17521 tgaaatataa attgattcat cacttaaatc agtcaatgta ttatttttaa actctgtaat
    17581 tgaaattatt ttagttccta ttaattttaa tcttctagca atattaagtg tattctcatt
    17641 ctcaccactt agggatataa taatcataac atcattttta ctgacaatat tgtaaaaaga
    17701 atcatcaaat gttcttccct caaaatcata tattatttct tgacatgata agaacattct
    17761 ctttatctgt tttgcaacca cattttgaat atcacctgaa ccataagcgt atattgttct
    17821 tgattcataa agaagtttac acaatgcatc ataatcttta tttttttgat cttcaacata
    17881 cttaattatt gaattgcatg caatatcaaa tgactgattt tctataacat gaaccttttc
    17941 tgattcccag cttaataata ctttaaattc actgaaacct tttaaatcta attttttaac
    18001 aaatcttgta atagttgcac gagacacatt acatttcata gcaagttcat ttattgacat
    18061 cttactacaa tgttttttat ttgcagttat atatttccat attaataaat ctgtctcatt
    18121 cagtttatta taatttaaaa aaatcatttc ttctaatctc ataaactatt atccccttta
    18181 attaaaaatt gtcataacta ttataccaat attcaacatt aaattacagc atattcttat
    18241 atttactatt tagcctataa aattgactat aaaaaataca gctataattg atgttaaaaa
    18301 aagtagtttt aattctattc ttttatctaa taattaatat tttcaaattc tagtgctttt
    18361 cttatgcaat tatcctcttt aaaatcagca gtccttttac aatcttttac tttaaattct
    18421 ggagtagttt taaattcctc atcacatact ccactcttat ttaaatacat acaacttgcc
    18481 attctaacta ttagaccact attttttaaa ttaaacaata cagggtctat acaccctcca
    18541 tctccaccag tagtttcacc aactaaagtt gcaaatttag tttctttaca aaacattgca
    18601 aatgactctg atgatgaata tatttttcta tcaacaagta agtatatatt acccttaaat
    18661 tttaaatcat ctgtagattt tatagtatag gaagtatctt caaaaccatt aaaatctttt
    18721 ataatttctt taggtgcatt ttcctttaca cttgctggta aactttctac aggatttaat
    18781 tttatatttc ttgcagttgt atattttttt actatttcat tattatttct ataaattcta
    18841 taaccattta ttttgacatc attttttatg agtttagata cgattccttg ccaataccta
    18901 tcacttccac ctaaattacc tcttatatca ataattaaag ctttatgttt ttttaaagtg
    18961 ctaatataat ctccaattat ttttaaatca ttatttatag acccattctt tgatgacatt
    19021 gatggtaagt acatatatcc aatcttacct ttaataacat cctttaaaat caattctttt
    19081 tttacaaaaa tatcatctgg catttttatt ttaggattca tagagtcata tctatctaca
    19141 acttctttat cattaagaaa atcataccaa tcattattag agtatgattt cttaaaaagt
    19201 tcatatcttt tcttgttatc tattacttct gtgtgcttat tatttaaatc agataatata
    19261 gatgacagtt cttctataaa ttcttcgtca ttttcagtac ttcttattct ttttatatat
    19321 tcatccttat tttctaacca atcaatatta ttaagcctct tatttacacc tagatatgga
    19381 tatcctgttt ttacaacatt atacacataa ttaaagtcat ctaccttttc ttcttcagac
    19441 aactgattct tttgagaata agtagaatta cctaataaga ttataaatgc taaaataatt
    19501 actaaaaata atactttctt tttcatgtaa ttctcatcca ctttcagatt ttatatttta
    19561 atacattgta tcattatttt attatcacct aaacaactaa aagaaattct tgaagatata
    19621 ttccactatt attctcttta aaatattttt atttatattt ttaaaattat ttcatgaaaa
    19681 ttataaaata acaatcatat tcaaatatct gttttacttc catatcctga aagtaaattc
    19741 ttatcagtga tatactaaaa aagatgtgct aaaaaattta tccattataa gggcttaaat
    19801 ttagatactt tactgatttt tatttattag ttgagaaaga tattgataat taaaaatatt
    19861 gataactata gaaatgatta ttttttatat aaatttaagg atataaacta attatactta
    19921 agtctatatc cttaatttat catgattacg aaaattttta caaacactta tatttgtctg
    19981 ttttagaata aaaacaagga catatcctgt tattttattc aaacatccaa gtttaattac
    20041 tacacaatta cttgttagaa attgtatcta cttcatatat atatctgtta agtatagctt
    20101 ctagcatttc ttgtcttcca gacttattct ttataactgg catttgcagt gcataagatt
    20161 ctaactcttt aaatccaacc ttaccttcta ctatatcttt acctataccc tcactaaaac
    20221 ttgcatatct ttctttagta aagttttcaa atacctcatc ttctaataat ttatgagcta
    20281 ttaacaatcc ctttgcaaat gtatccattc ctgcaatata agctaggaat aagtcttcta
    20341 cctcaaaaga tgctcttctt actttagagt caaaattaaa tcctcctggt gctattccac
    20401 cttgctttaa gacttcatac atagcaagtg ttgcatcata tatatttgtt ggaaattggt
    20461 ctgtatccca acctaataat aggtctcctt gatttgcatc tatactacct aaaacattgt
    20521 ttattctcgc tatatttaat tcatgttgga aagtatgccc tgcaagtgtt gcatgatttg
    20581 cttctatatt cactttgaag tatttatcca gattatactt tcttaaaaat cctaaaacag
    20641 tagcagtatc aaaatcatat tgatgtttag taggctcttt tggcttaggc tctattaaga
    20701 attgtccagt aaatcctatt tccttagcat aatctacagc catttgtaag aatcttgcaa
    20761 agttatccat ttcaagttct gtattagtgt taagtaaagt ttcataacct tctcttccac
    20821 cccagaatac aaaattttct cctcctaagt acttagttat ttccatagct tttttaacct
    20881 gagctgctgc atatgcatat acatctgcat tacatgttgt agatgcacca tgaacaaatc
    20941 ttggatttcc aaacatattg gcagttcccc ataataactt tatacctgtc ttttccatat
    21001 tagcttttat atactcaact atttcatcta gtatcttatt tgtctcttct aaatctcttc
    21061 cttctggagc tatatctctg tcatgaaaac agaaatactc aattcccatc ttagacataa
    21121 attcaaatcc tgcatcaact ttaatctttg ctatctcaat tgggtctgtt atattattcc
    21181 aaggtctctc tactgtacct actccaaatg ggtcattacc ctctccacat aaagtatgcc
    21241 aatagctcat tgcaaatctt aagtgttctt tcatagtctt attccctact acttgttcag
    21301 gattgtagta tctaaatgct aactcattgt tagattttac tccttcaaat tttatctgtc
    21361 ctattccttt aaatatttca ttcatttcat gtcctcctaa tattttacac atataatata
    21421 tgtagtatgt tttcttaatt taaaactatt ttctatcttt attttctttt tattttaagc
    21481 tatcttgtct atttattttt gcaattaact gcttcatcta acaacctaaa gtcgtctttc
    21541 aaagatttgt atattttttt atacaatctg tgatatgtct tgtatatatc tacattttca
    21601 cttattggtt ctaatttagt tgaagttttt attatagtat cacaagcttt ttgtatattt
    21661 tcatatattc cagctcctac taatgcaaga attgcaactc caagtgctgg tccttcactt
    21721 gaatttatag tatatacatc ttgattaaac atatctgctt gcatctgtct ccatacttta
    21781 ctttttccac ctccacctga ggctcttact tcattaacgt ctatattaag attttctatt
    21841 atatccatac agtctttaag actatacgaa acaccttcca ttattgacct tgcgatttct
    21901 tttttagaat ggattggaga caatccaaag aacacgcctc ttgcataagg gtcaagatga
    21961 ggagttcttt ctcccatcat atatggtaag taaagcaatc cattacatcc tgttggtact
    22021 tgacttactt gattatttat aatatcatac acatcttcgt ctaaagaatt tgaaacttcc
    22081 acttcacttt gacagaaatt atctttaaac cattttaatg acaatccagc tccttgagta
    22141 actcccataa catgccatgt atttggtatt gcatgacaga aagtatgaac tctaccttct
    22201 ttatcaattt ttggctcttt tgtataagca aatactacac ctgatgtacc tatagtggac
    22261 gaaactactc catctttaac tattccattt cctatagctc ctgctgcttg gtctccagct
    22321 cctcctacca ctattgttcc ttctttaagc cctgtcaaac ttgctacatc tttagtcaca
    22381 taacctgtaa cctcttgtga ttcataaact ttacctaaaa gatttttatc tatatttaac
    22441 ttttctagta cttcatcgct ccaatctctt tttcttatat cgagcaactg cataccactt
    22501 gcatctgata cttctgtagc atattcacct gtcaatttgt atcttatata atcttttgga
    22561 agaagtattt tattagtatt tttatatata tctggttcat tgtttctaac ccataatatc
    22621 tttgatgctg taaatcctgt aagtgctgga ttagcagtta tttcaatcag tctttcttca
    22681 cctactaaat cagttatctc ctcacactct tttactgttc gttggtcaca ccatattata
    22741 gattttctca atgttttgcc ttcactgtcc agcataacta gaccatgcat ctgtcctgat
    22801 aatccaattc cttttataca ttcactactt atatttgaac tttgaactac ttctcttata
    22861 gattcgacta cagctttcca ccaatcattt gcatcctgtt ccgcccaacc tgatttttct
    22921 tgtataagag gatattcata cgtacaactt tttatagtat ttcctagttt atcaaataaa
    22981 actgttttag tacctgatgt accaatatcc acacctaata catactccaa cttcatccct
    23041 ccttgaatgc cacttcataa ttatcgtttt cctatcctaa tttttttata ttttaggtat
    23101 attttacttg ctttattttt gttagtatat taaactaact tatgaactaa taataaaata
    23161 tttaattatc tttgtcaatg caaaataacc ttttattttt gtgtaaaaag tattacaata
    23221 ttattttaag gaggaatatc aaattggtta ctgataaata cactattcga gaaatgaacg
    23281 aaagattagt acttgaacaa attataaaaa atggtcctat ttctagggca agtatagctt
    23341 ctactatagg attgaataaa gcaactatat ctgctattac caaaaaactt attgatgaat
    23401 ctctagttca tgaaatagga attggaaata gtactcatag tggcggaaga aaacccatat
    23461 tattagtatt taataaatgt gcagggattt ctttaagtat ggatattgga tacgactata
    23521 tattttcttc tatgtcttac ttagatggaa ctattataaa ttctaagaaa ttaacagata
    23581 tacaagtaag taaagacaat gttatacaat tgatagatga aattataaat agttataaca
    23641 tatccaaaat agacactcct tataaggtaa tcggtctcac tttggctata catgggatta
    23701 cttgtgaaaa taaagtatta tttactccat attacaatct taatgagatt gacttatata
    23761 gcatccttag caaaaaatat gactttccta ttcatataga aaatgaagca aacttaacag
    23821 ctttagccga gaatactttt tcaactgtac atgactcact actcagtctc agtattcaca
    23881 gtggatttgg ttcaggaatt ataataaata ataaattata tagtggcaga aatggtatga
    23941 gtggagaaat tggtcatact atcatcatgc ctaatggtaa actatgtcct tgtggaaacc
    24001 gtggctgttt agaacagtat tgttcagaaa aaaaggtatt tgaacagctt tcttctttag
    24061 aaaatattcc taagatagat tctgatatag taaagaaact atattatgag gataaccaga
    24121 ataccaaaaa agttatccat gaattttgta gctacttaac aatagctata aataatgcta
    24181 ttacaaccta tgctcctgag ataatatatt taaatagcca aataattagt gatatccctg
    24241 agatattaca gattacaaaa gatatgttag ttagctcttt taacaaagga ataaatatag
    24301 aaatttcatc attagggtct gaggcatctc tatatggtgg ctctgctgta aatatcaaga
    24361 gctttttgaa tatacaaaat ttaactttaa ttaatgaaat ataagggtta tgactatata
    24421 cagccataac cctttaaatt atacattcta aaattaaatt tattacatag attacgcaag
    24481 attagctata tcttttaaaa attgtatatc aattaagcat taacataagt attttgtttt
    24541 aaatattaat ataatcttcc tactcatcat cagataataa acttgtattt tgttcaacca
    24601 tagaaatatt atctttacct attttctcta attttactac caattcattt gtatctaaaa
    24661 aagctgattc aataaccata cctaaattta gccctgaaat aattttaatt cgactatcat
    24721 tattggctac taataaagca gcattgcatg gagttccacc tagtaaatct gccattataa
    24781 ttatttctga actttccttg tacacttcta aagctttttt taatttttct attgttcctt
    24841 ctattccatc atcttgtttc atatttacag taggataatc tatagaatcc cctattatca
    24901 tttttgcaga atttaatact tcttcagcca tattaccatg actcatcaaa ataatactat
    24961 tcactttaat acacctcttt aaatctataa gatgtctaca agataagttt tcttatcatc
    25021 aggcgtcatt atagttgtaa tctcaattcc ttcatctaaa agtcttttaa atgcttcttt
    25081 ctcttcttca gtaacactga tattaggttt tagcactgtt gtatcttttc tacttgacat
    25141 attacctaca tttatattat gcattttaaa tcctgcctct acaaatggaa taaggtccac
    25201 aggagactta acaattagta atacattttg cccttcatac tttcctgcag taatattttt
    25261 tatagcagtc tcttctgtaa ttatagatga cgctatacct gcaggtgtag ccattcttaa
    25321 aacttgtttt tgaacagaat tgttagctac tgcatcattt actaccatta atcttgtaac
    25381 acctaattta ttactccaca tagttgcaac ttgaccatgg attaaacggt catctatacg
    25441 aatattttta atacctttca tttccatatc taataccatc ctttccttat tttaaaatac
    25501 ctaatgcata taaaattatt gaaacaacca ttactcctaa aattaagtat ataggtttta
    25561 tattcttttt aagagcccaa taaacaatca caactaaaat tgcaggaact aacccaggca
    25621 taatttggtc taatatttct tgacctttca ttgtaaaatc accttgttga aagttcaaag
    25681 caacattagc ttttactact gaaggtatta aggcacctac aaccattaat cctaaaatag
    25741 atgtgctctc agttaaaatc tttaaacttt tttcaattga agatattagc ttagtacctg
    25801 atgtataacc agcattaaat agcggtatac ttaataattt caatgctaca ctcactataa
    25861 tccacagtaa gattccaaat ggacttccat ctatcgccat attagctgct atagctccaa
    25921 aaattgtcca cggaataaca acaaataaac tatctcctaa acctgctact ggtcccatca
    25981 agcctgtttt aattgaagca acagcttctt cagtctcttc acttgataat tcctccattg
    26041 ctacattaat acccaaaata taaggtgcca cccaaggatt agtattaaaa aactgtaaat
    26101 gatttaatac agcgctttgt aatttttctg gattatcttt gtatactact tttaaaaaag
    26161 gtagtactgc ataacagtat gccaaggcct gcatcttctc ataatttgaa gaaacagaac
    26221 tagtaaaaaa ccagcgccat aagacacttt ttttcacttt ttcatttgat ttatttaatg
    26281 ataccttatc actcatcttc gtccatacct cctactacag tatattgttg tgcttgattt
    26341 tccaaattct ttttgtaaat tataagtgca attgctccac caattaaagc tacacctaat
    26401 aatggtactt tcatatatac tgctaaaaca aaaccaatta ctaaataact aaaatactcc
    26461 ttagcaggaa gatatctaag taataatcct atccctactg ctggaagtag tcctccagct
    26521 actgttaaac cacctgttaa ccattcaggc gtatatttta aaattgtatt aattaaagaa
    26581 ggtccaaata ttacagatag agcaactgga atacctgttg tagctgctgt taataacact
    26641 cctaacatct gcatatatgt tatattttta taatttcctt ttttagctcc atcttctgct
    26701 ttatgcacta accatatacc gatagtattt ctaagaacat ctaattgtac catcaataaa
    26761 gcaactggaa tacctataga aattcctaca gatgcttctt gcttagttgt aatagttata
    26821 aatgtggcta ctatactggc tgtctgataa tcaggtattg atgctccacc aaaattacta
    26881 atacctaatg ataatagttg taatgtacct ccaatatata accctgtatt tatatctcct
    26941 aagataagac cagtaataaa acctgctgtt actggttgat atgttccaaa cattgttgaa
    27001 tttttttcat agtttataaa aaatccatat agtacgatta ataataattg ccataattgt
    27061 ggctccatta tgatttcacc tcactcaaaa tattttttaa ttctttaaag ttataactag
    27121 aatcttgctt taagaaaata ggaatctctt ccagtgggca ttcaatttca taagttttcc
    27181 caccttcata actaactcca ctaaatatat tttcccatgt atgtcctttt ggtaaatata
    27241 cttttctact tctatcttta taattcatta ttggagctac caaaatttca tctccaagca
    27301 tatatgtatt atccacctcc caagttttat catcttcagg aaattcataa aatagagttc
    27361 tcataactgg tgttccatct tcatgtgctt ctttcatcag tttttttaaa taagttttta
    27421 aactctctct aatttttata aatttcgtaa gaattacttc aacttcttca ccataagacc
    27481 atatttcatt aggagctcca gttaccattg agcctccacc tttatttgac aatggtttac
    27541 tatgaggcaa tctatctcca tgcattctca aaattggtga aaaagtagca tattggaacc
    27601 atcttaccat aagttctcta aactctgggt ctttaggatt tcctccatgg aaacctccaa
    27661 tatcagtagt ccaccatggt atacctgcca gtcccatatt taaccctgta tttacttgat
    27721 tattgaaagc ctcaaaacta gagtcaatgt caccagacca tactaaagct ccatactttt
    27781 gagctcctgc ccaagctcct ctcacaagag ttacaacaga ctctatgtca ttatgtaagc
    27841 catcataaat catttttaaa taatcaatag gataaatatt accacaactt aaaacattac
    27901 cttttttata tctatagtta tcaaaatcat acacagaata gccaggttca gctacatcta
    27961 accaataata atcaattcca agttgtttat aatttttatc tattctatcc caaacatatt
    28021 ctcttgcatt ttcatttgtc atatctacaa agactgtatt tccttgaatt tgcattgtca
    28081 ttctaacacc acgattaaca ttgactaaat aaccattttc taaataatca ttgtagtttt
    28141 ctgcatcact ttgaactgta ggccatacag atactattgg ctctactgaa tactcttctt
    28201 tcaatttttt aactaactct ttaggatttt tccaataatc taaatcaaat ttatattcac
    28261 cttgcttagg ccaatgaaaa tagtcaattg caattgatga aagcttaatt cctcttttag
    28321 aatattcctt cacaacatct aaaacttctt ctgaagttct atatcttagt ttactttgcc
    28381 atagtcctaa aaggttttca ggcatcatag gaacagttcc tgtaacttga gaatagtttt
    28441 tattcaactc ttttgggctt tctccacaag taatccaata atctataaat tttgtagaaa
    28501 acatcttcca ttctgtcata tttttagcaa aagatacttt cccaataccc ggattgttcc
    28561 ataaaagtcc ataacctaga ctagaaatat aaaaaggaac tgatatttga gaatttcttt
    28621 gggctaactc caaaacagta ttctttaaat ctaaaaattt atgttgatat tgtcccattc
    28681 caaaaatctt ttcagaaggc tcagattcaa atctagtagt tacttcaaat tctccactat
    28741 agcttggtcg atattctctt gattttaact ttagagtaga gttaaaatct ttggtaattt
    28801 caattgtccc tacatcttct ccaccatcat catgttttac agctcttaat cgaatatatt
    28861 ctttcaataa tatttcattt ttatcattgt aaaaagtaat tctatctcta tggtctaaga
    28921 cagcttttat ctttccattt ttaatgattg cacttccatc ttcattttta ttaattgtaa
    28981 tatcatcttt atttaactta ggtttttcgt taagagcata gttttcatca acaaagtttt
    29041 ggtctacaaa cgacctgata cgtaaagagt tttttcccca tgcttctatt cgcaatagtt
    29101 cattgtcaaa tcttttttca atatagtctt cagatatttt tatcacggct acctcctaaa
    29161 tcaatggtat aatattctat ctttgtgtag cttaagttaa ataatgtcat ttagtttcgt
    29221 tatttataaa ttagatttta tataattcat ttgtacatta aactaactta ccaataatat
    29281 actttatttg actttgtttg tcaatcgctt tccggatttt agtaaaattt tctaatatat
    29341 tgatggaaag tcatccttaa tattttttat ttatactttt taatatctgt gatgatacct
    29401 atatacatct agatttttaa aacacataca ggaataatgt ttacctctta tgtaaacaat
    29461 actggactta aaaatatgta tagcgacctt ataaatacag atgataatat attagatatt
    29521 attcaaaaca aaggatttta taattataaa taatttatga aattatttaa gaaaatatat
    29581 aattgtacac caagtcaaaa acggcaataa ataaataaaa ctaaaaaata cgttgtctca
    29641 aaatatagat aaatttattt ttcatttctg aaatgattaa aatatatttg atttaatata
    29701 aaaaagagtg tctcatgaac taaaaagttc acttttagac actcctttat atattattta
    29761 cttcttaatt catttattac ctctctacat tttctttcag ctagtgaata catctgtact
    29821 aacatagcta ctgtaagtac tattggtata ataccattta tagaacgaat accacacaaa
    29881 gcttgtgaag tttgagaaat atttgcaaca tatcctgtca tagatagtac caaataccaa
    29941 tatgaatatt aatgataata ttgatttttt taaatttttc tttaggagta ctaattcctc
    30001 cacttaaaaa tgcttcatgt actgtccatc agtcatattt ttctaccaaa tatgatgttg
    30061 tttcataatg agacaataaa tatttataca tttacttacc ttctctaatc tcatcaaatc
    30121 caactatagg tatctctaaa ttatactctt taagtagctt ttctagctct tctataaaaa
    30181 tacctttccc ttccaccatt tgtatatcat ttattttatc catcaattca ctacaaccac
    30241 ttaactcacc aaaccaacgt ttagaactac atgtcttatt atatccacaa cttgggctac
    30301 catctattgc aataatactt tttatatcat aattattttt taaatattca ccaaattgtc
    30361 ttacataagg tattaacatt tgcctacatt gttctctaaa aaacaaattg tcaaactgtt
    30421 cttttacatg tccccaccta tttattccat acataatcat ttctggacat ggtagttgaa
    30481 ttattccata ccctttattt attaaatcta ttgctatatt ttgtgctcct ttaaattctg
    30541 acaacccctc tactttagaa ttacaattta atatacagtg acatactact acaatctctc
    30601 tatttctctt cataataatc tccttagtta ttcttaattt taatgatact acatattttg
    30661 ataattatat tatttatttg ttctatattt ccaatattct atatagaaat atacaattag
    30721 taatctttta taatctatac tatgataaat gtatatattt ttttaggagg tattataatt
    30781 ggatactaaa aaagtcacat tttcttcaat gtgtatagtt attaatattg tcttaggtat
    30841 ggttatatct atgataaata taccattatt atttttagat acagttggta ctgtacttgg
    30901 ggctgtcgta ttaggtcctt tttggggtgc tcttattggt gggtgtacaa acttagtttt
    30961 aggtgttata tcaggaccta caaatatacc ttttgctctt gtaaatattg ttcttggtct
    31021 tattgttgga tatatttcta ataaaaaagg ttttggttat aaagaagcta ttattacagg
    31081 cgtaatttta tctattgttt gtcctttaat tggtactcct atttcagttt tattgtttgg
    31141 tggtttaagt ggaagtggtg ctgatttatt ggttggtttt ttagttcagt ctggtcagaa
    31201 gatatttact gctgcgttct ttccaaggat tcttagtaat atagtggaca agcctttgtc
    31261 ttgtattatg gtggtatatt ttataagtaa aatgcctaaa ggttttataa aacaatatag
    31321 taaaaatacg tatagtcatt cttagtaaac tgtatatatt atatgatttg ctaaaggtta
    31381 taatgagttt atacacattt atttgtataa tatattttgc actttattat tcattaattt
    31441 tagtattggt gatttaatta tgtgttatct ttattaattt aattaaatac aacctcttaa
    31501 tataaattta ggttctaaaa ctgtattaag aggttatatt tttattttat aatatattta
    31561 aagttttatg agaacttata gaatatacat tttttatttt agttaaaatc tctctctaaa
    31621 aatcatattt tgcaagtttt cttatactaa caaatacatc ctcattttct ataaacaact
    31681 catacatatc tttaaactct gatatatcat atccataaga gttattattt cttaattttc
    31741 taagcgtttt taataatata ttctttatta tatatttctc atttgacatc ttaaatatac
    31801 aaaataaagt aagatatcta ctatagatat cttactttat tttcatttat aattatattt
    31861 tattattaac actttactgt ctctattgct tttcctacac atccacaaac ttccattcta
    31921 ctcttaacaa aatctttaac tgactctaaa ccttttttac catatttctt agggtcaatt
    31981 acctctgggt cagaattaag cacatcctta accccattac tataagcaat ccttaattct
    32041 gttgcataat taaccttgca aatccctctt tttatagatt ccttaataac ctcatctggt
    32101 attccagacc caccatgtaa tactaatgga actgatacaa cctctctaat ttcagataat
    32161 ctatctaaat caagctttgg ctctccctta tatagcccat gtgccgtacc tattgctact
    32221 gccaaagatg atacccctgt tctctctaca aactcttttg cttctaatgg gtctgtatat
    32281 gctccaccat caccaccatc taattcgtct tccttacctc caactttacc taattcagct
    32341 tcaacaggta tatctgaagg catacatgca tctacaacag atttagatat tgaaatatta
    32401 tcttcgtaac tttcatgaga accatcaatc ataatagaag tatatccagc tctaagtgcc
    32461 tgcatagcta gtccaaagct tgaaccatgg tctaagtgca ttgctactgg aacacttgcc
    32521 ttctctgatg ctacctttac attcgctaaa taataatcta gtcctgcata ctttattgtt
    32581 gaaggtgttg tttgcaatat tactggtgat ttaagctctt cagcagcctc tattactgcc
    32641 ataaccatct ccatattttc aacattaaat gcacctactg cataatgccc ttcttgtgca
    32701 tctaataata tttgtttagt tgtaactaat gccataatct ctcctcctat atgtttatac
    32761 tgatttgatt tcttcgttat ttaattttga tactatttcc ctacttgtct tgctatttaa
    32821 taactcattt aaaaattctt cattcattaa ttttgcagat aattgagata atgtttttaa
    32881 gtgtaaatta tcaccattgt ctgtagctgc tatcataaat ataatatgta ccaagcttcc
    32941 atcttctgat tcaaagtcaa acccttcttt tgatacaccc actgctacac gtggtatttt
    33001 tactgcatct gtttttgcat gtggtatcgc tatccccata ccaaaacctg tagatgatat
    33061 ttcttctctc ttaaggattt cttctttaaa cttattttta tctaaaagaa caccagattt
    33121 atcaagtact ccaaccattt catcaattac atctgtttta ttttttccct taacatctaa
    33181 aattactgtt tcttcgctat ataaagagat attattagat acgttttccg attctgcact
    33241 tttattttta cgttcatctt tagttgcatt tgtattttct atagtatttt tctcatttac
    33301 tactttaggt gtaaccatca tataaagtac agcacctact aatgaaccta caaatataca
    33361 ccctacccat aaaagtggct tattgactaa tcctagaacc aaaaaccctc catgtggagc
    33421 tggtacttct attttcatta tataagttaa aattgatgat attgatgatg caatcataag
    33481 tactggcaac acttttaatg gattttttgc tgcaaaaggt attgccccct ctgttatatg
    33541 agtactacct agtatataat tgattaatcc tgcggctcta tcttcttctg taaatctgtt
    33601 tttaaataaa gtggatgcta ttgctattac caatggtggt gttatacaag ctgcagatac
    33661 acctgccatg aataaataat ttccctgtcc tagaagcata gttccagtta catatgctgc
    33721 tttatttact ggacctccca tatcaaatgc tgacatacat cctattacta ttcccaagat
    33781 tatagggctt gaattttgca accctgatag aaagttcatc atagcattgt taagtgctga
    33841 gcatggtcca ccaagtaaca acatggctac acccacaaga cctacactta caataggaat
    33901 tataaaaata gatttaagac cttctaattg tcttggtaat tttcttaaca aatgcacaaa
    33961 taatttaact aagtaacctg ctaaaaatcc tgatatgatt ccacctaaaa aacccgaacc
    34021 tgtatcgaat gatatcattc ctccaacaag tcctgccaac ataccagctc ttcccgatat
    34081 agattgagct ataaatgctg ccaacacagg aaccatcaaa ttcatagaat atccaccaac
    34141 agattttaat gtagctgcta tttgattata ttgttctgaa tttgggtcaa atgaataaat
    34201 cccccataaa aatgatatag ccgtcaatac tccacctgct actactaaag gtaacatgtg
    34261 agatacacca ttcattaagt ctttatataa cttttgacca aacgataaat ttccttcttc
    34321 tgcaatttta ctgcttgatg agtttccttt tctaatagct acttttccat caatacacct
    34381 ttgtataagt tcatcagctt tatgtatacc ttctttgaca ggtacttcta ttactggctt
    34441 cccattgaat ctgtccatat caacattttt atcacaagca actattattg catctgccat
    34501 ttcgatatct tttgctgtta acttattttc tacacctatg gctccattcg tttcaacttt
    34561 tatatcacac cctaattccc tagctgaatt ttttaatgct tcttctgcca taaaagtatg
    34621 agctattcca gttggacaac ctgttacccc tagaatcatt ttctttccca tttttatacc
    34681 acctttctga ctcttatatt ttttatataa tcttccacat tctccaaatt acctatacca
    34741 ttactttcag ccacacttgc tcctgttgct gctgaccttt ttaatgcttc ttcaatatgt
    34801 tctggatttt ccaaccatat acttaaaaaa gcagctaatg cagaatcacc agcacatgca
    34861 gagctgacta cggtaactgg ttgggcacta gcataataaa ttgattctcc attataaaaa
    34921 taagaacctt tttctcctaa agttagtaat atattttgag cgccttttaa atgcagtaat
    34981 ttaagtacat ccttaatatc ttcttcatct cttacaataa ttccaaatat atctttgatt
    35041 tcttcatcat ttggttttat caaatatgga ttatactgta gtaactcctt taattttggt
    35101 gaacttatat cgagtattgt ctttatactt ttatttttac aaatcttaaa tatatcctca
    35161 taataattat catcaatacc aggaggcaga cttccactta ttgataaata acttatatct
    35221 tctaatgact gtatcttctc tagcatatct aatttttgtt tttcgtttac atacgaccca
    35281 gagtttacaa atttaaattc atcacttcca tcatttaaaa atatatttat acgagttgta
    35341 tcttctaccc aagtaggcaa tacattaaat cctctctttt cactttcttc aactatatat
    35401 tttcctgaaa aacctccaaa aaatcctaat atcttagatt ctataccata atgacctagt
    35461 acaaatgtta catttaatcc ctttccatta ggagaatata cagcatttga tgtacgattt
    35521 actaattttc tctttatccc attagtagat atattcatat ctattgcagg attcgtagtt
    35581 aatgtatata tcattgtctt cccccttgct tgtgctgtgt atgttttcat tataataaaa
    35641 aagtctatga aaatcttttc acaaatagaa aatcgatttt catagacttt tcaatttaat
    35701 tttcagaatt attatatctg accatataat ctatgattat cctagatata atatacaagg
    35761 gcaatctaga ggatacttca tattcagaaa ttgatatatg ttcatgtttg tatcctacaa
    35821 tatataaatc tgaatacttt ataagttttg cactttcact acaacaacaa gttataactt
    35881 tagctccaac taaattggca ttctgtgctg attctacaag ctctatggta tttccattca
    35941 atgaaaatat aattataagt tcgtcttttt ttattgattt agattttatt ttcattatgt
    36001 tggggtcatt tataaaaaca acattaaaat caagtaattg cagtttaaga ctaaactctt
    36061 ttgctacata ttcagtaagc cctcttgcta aaatatatat cttactagat tgatttatag
    36121 tttttattat attaattagt gtagttaaag atatttgttc tataactctc tgtgcttcta
    36181 taaaggattt atttattata tctcccatat tttgtatatc atcattttca tcttttactg
    36241 ttaatctata cctaagttca ttgaatccat ttaaaccaca ttttctaatt gctcttgaaa
    36301 cagttgctgg agatgaatat gtttcatgag caatctctac tatagataat tctggtaata
    36361 ttgattcatt ttcatttata aatttaatta tttcaagttc cgtcttcgtt agattttcag
    36421 aattactttt gtttagctca attatcatat gttatctccc cttatataag ttttatttgt
    36481 ctaaatttac cgagtaatat aatatattac ctcttaacta tttattt
//
LOCUS       DAFNNB010000020        35420 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0020, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000020 DAFNNB010000000
VERSION     DAFNNB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35420
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0020"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            56..1057
                     /locus_tag="L3M60_000905"
     CDS             56..1057
                     /locus_tag="L3M60_000905"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBN6225599.1"
                     /translation="MKKVNVAIVGATGMVGRTFLKVLEERNFPIENLFLFSSSKSAGS
                     KVMFCGKEYIVEELKETSFTDRGIQIALFSAGGSISEKYAPIAASNGILVVDNSSQWR
                     MNPDVPLVVPEVNPEAIKNHKGIIANPNCSTIQAMVPLKALDDKYKIKRVVYSTYQAV
                     SGSGVKGVEDLEKGMNGNPTGFYPHQISYNCLPHIDSFTENGYTKEEMKMVNETMKIL
                     DNYDLKITATTVRVPVKNGHSESINVEFNNPFELDDLVKTLEEAPGVVVVDNPAKNEY
                     PTAVEFDDRDEVFVGRIRRDFSVDNGVNLWVVADNIRKGAATNAIQIAEMALSYDLV"
     gene            1174..2061
                     /locus_tag="L3M60_000906"
     CDS             1174..2061
                     /locus_tag="L3M60_000906"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBN6225600.1"
                     /translation="MIFKGSAVALVTPFTKDNKVDFDKLGELVEYQIANGTDAIVSCG
                     TTGEANTMTDEEQLATIKYVVEKVNKRVPVIAGSGSNDTMHSVNLSQEAEKLGVDALL
                     IITPYYNKANKAGLKRHFETIANSVKLPIILYNVPGRTCVNISPSLIVELAKIDNIVA
                     VKEASGDLGQVAEIASLVPDDFAIYSGNDDTILPLLSLGGQGVISVLANVCPQETHDL
                     VAKFFEGDIEGSRKLQLGMDALIAALFIEVNPIPVKTAMNLLGFNVGDLRLPLAEMDP
                     ANLEVLKKELVNFGLKVNA"
     gene            2129..2878
                     /locus_tag="L3M60_000907"
     CDS             2129..2878
                     /locus_tag="L3M60_000907"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBN6225601.1"
                     /translation="MLKVIATGYDGKMGKILADTIREDSELELVCVAARGLDSYEDDL
                     KIYEDMSTIVEEADVVIDFSHHSNLDNILSYVLKTKTPLVIATTGYNDEEMNKIHEAA
                     KVIPVFQSYNMSLGVNVLVKLVKDAAKLLEGFDIEIIEKHHNRKVDAPSGTAVMIANA
                     IKEVLPNVENNYGRYGRSAKRQPNEVGIHAIRGGNIVGEHDALFAGDNEVLTISHQAQ
                     SRGIFASGSIAAAKYLVKQKPGFYNMDSMLG"
     gene            3060..3776
                     /gene="dapD"
                     /locus_tag="L3M60_000908"
     CDS             3060..3776
                     /gene="dapD"
                     /locus_tag="L3M60_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HBN6225602.1"
                     /translation="MVNLNDAYEIARYIKEVKKKTPVKVYVNTNSLHFQSTDKFKVFG
                     CNGSYIFVGDYEDIMEVLDKHGNDIIDTHVEYDRRNSAIPLKNTLSEHARIEPGAIIR
                     DMVTIEKNAVVMMGAVINIGAVIGEGSMVDMNAVIGARGTLGKNVHLGAGAVVAGVLE
                     PPSATPVIVEDDVLIGANAVILEGVRIGKGAVVAAGAVVTTDVEAGAVVAGSPAKVIK
                     MKDEKTADKTKLMEDLRNLD"
     gene            complement(4059..5048)
                     /locus_tag="L3M60_000909"
     CDS             complement(4059..5048)
                     /locus_tag="L3M60_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HBN6225603.1"
                     /translation="MLKEEKTYQEKEAKVFNGVAALIMIVLFLVVSIGIMVFGIVKLD
                     SGFTGIGSLMLIFGLIFIVVDFILFFGLKMINPKEAIVLVLFGNYYGTIKKEGYYWVN
                     PFCSAINPAASRINVSKSSSDEKVDISTNSRGKKVSLKTMTLNNEKQKVNDLLGNPII
                     IGVVVIWKVIDATKAVFNVDNYNTFLSIQCDSTIRNVSRLYPYDVSEDGDEKSLRGSS
                     QEIADRLKDELQSRVDIAGIEVCEVRITHLSYAPEIAAAMLQRQQAEAIIAARKKIVE
                     GAVSMVEMALTNLSENNVVTLDEERKAAMVSNLLVVLCGNKDAQPVVNSSSIY"
     gene            5491..6246
                     /locus_tag="L3M60_000910"
     CDS             5491..6246
                     /locus_tag="L3M60_000910"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBN6225604.1"
                     /translation="MLKVIISGYNGTMGRVLAECVKKDNELKLVCGVARSADEKSHHS
                     DTKLYKTMTDVTEKADVIIDFSHHETLEPILSYALKNNTPVVLATTGYDESDLSKIEE
                     ASKNIPILLSSNTSLGVNILIKLVKDASKMLEGFDIEIIEKHHNRKEDAPSGTATMIA
                     NAIKEVLPESTNTHGRYGRDCKRKANEIGIHSIRGGNIISDHDVIFAGDNEVLTLSHH
                     SQSDEVFANGSIVAAKFLVGKQSGLYKMSDILG"
     gene            complement(6520..8205)
                     /gene="bclA2"
                     /locus_tag="L3M60_000911"
     CDS             complement(6520..8205)
                     /gene="bclA2"
                     /locus_tag="L3M60_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA2"
                     /protein_id="HBN6225605.1"
                     /translation="MSDISGPNLYQDVGPTGPTGPTGATGPTGPTGPRGATGATGANG
                     ITGPTGNTGATGANGITGPTGNMGATGPNGATGSTGPTGATGATGANGITGPTGNTGA
                     TGANGITGPTGNKGATGANGITGSTGPTGNTGATGANGITGPTGNTGATGATGPTGLT
                     GATGATGANGITGPTGNTGATGANGVTGSTGPTGSTGATGPTGAVGATGANGVTGATG
                     PIGATGPTGADGEVGPTGAVGATGPDGVIGPTGPTGATGATGANGLVGPTGPTGATGA
                     NGLVGPTGPTGATGVAGAIGPTGTVGATGPTGADGAVGPTGATGATGANGATGPTGAV
                     GATGATGVAGPIGPTGPTGANGVAGATGATGATGANGATGPTGAVGATGANGVAGPIG
                     PTGPTGANGVTGATGNTGATGANGATGPTGATGATGVLAANNAQFTVSSSSLGNNTLV
                     TFNSSFINGTNITFPTSSTINLAVGGIYNVSFGIRATLSLAGFMSITTNFNGVAQNNF
                     IAKAVNTLTSSDVSVSLSFLVDTRAAAVTLSFTFGSGTTGTSPAGYVSVYRIE"
     gene            complement(8562..9101)
                     /gene="hpt"
                     /locus_tag="L3M60_000912"
     CDS             complement(8562..9101)
                     /gene="hpt"
                     /locus_tag="L3M60_000912"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBN6225606.1"
                     /translation="MDIETKKWEVLYSEEKIKDKLKELGAIIEKDYKDKNLMVVSLLK
                     GSFIFCADLVRNINLPLRVNFMTTSSYGNNEESTGKVKVVSDVTTDIAGYDVLVVDDI
                     TDSALTMDFVLKHLKAKNPASLKCCVLLDKPSRRKVDLVPDYCGFEIEDKFVVGYGFD
                     FGDYYRNVPYIFNVTDEDR"
     gene            complement(9430..10737)
                     /locus_tag="L3M60_000913"
     CDS             complement(9430..10737)
                     /locus_tag="L3M60_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBN6225607.1"
                     /translation="MRDIRKELVEMLKAEVKPAVGCTEPVALALACAKAKELLGEEIV
                     ENRMLVSPSIYKNGMCVGIPGTERLGLKIAAALGIVGGHSENGLSVLETLTKEEVKIA
                     EDYMDNTPLSITPADTREKVFIEVVLKGKNHIAKVRIRTKHDNFTFLEKDGEVLLDNE
                     PKVSASNDSAEKVESLMDTVTIQELIKNVEGIDFKDIEFLLDGVKMNEEMAEYGLKQK
                     TGIGVGYGIKKSIEEGLLGNDVINYAMMLTAGASDARMAGVKMPVMSSNGSGNHGLTA
                     ILPIVAYNKKFPQSDERLAKALAISHLVTGYIKNYTGRLSAVCGCGVAASTGATAGIS
                     WLMNGTEKQIEGAIENMIADLSGMICDGAKAGCALKLSSAASAAIQSAIIAKQDCFVP
                     PLNGIVGSSVEQSIQNLGRVSDKGMSITDEIILNVMNDMNKVD"
     gene            complement(11161..12936)
                     /locus_tag="L3M60_000914"
     CDS             complement(11161..12936)
                     /locus_tag="L3M60_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6225608.1"
                     /translation="MMIMIGLEYIKSDIQNIAEAIVSVLNIDVTIVNKDLFRIAGTGV
                     YIDKIGEKVDKYTAFKKSLTEQITILIDDPKSSDICRECYKSGACVEFAEVCCPIICD
                     GVSHGVIGLIAFTKEQAQIIENNKSGLINFLGKMADLISNKLKAQIKTYELELEKKKL
                     ETLLNNMDKAVVSIDVDGKIDKYNSKFKELFNIKDNITGKNIFVELDFIKKPSINNFK
                     KDKSCSFFYKRYGYDSKGIYNINKIVLKNELKGYVIDFIDKRDAIKNYNKMNKDYRIK
                     LENIIGESVAIRNSKKEALIASKSTSTVLITGESGTGKELFARAIHNHSNRVDNPFIA
                     VNCAAIPDNLLESELFGYEEGAFTGAKKGGKLGMFEVAHKGSIFLDEIGDMSLHLQSK
                     LLRVLQEKELNKIGSKSNVSIDVRIIAATNKDLEKMVQNGTFREDLYYRLNVIPIKLP
                     SLKERKEDIPLIIKHMVKEYSKKLDKNVIDIDSRVVDAMMEYKWPGNIRELQNIIEYS
                     VNMSSSSIITMNVIPQKIKNTSVEELEVRSDRIIPLDELEKIEIIKALNKYKDYKKDK
                     ELTAKALGISRATLYRKIEKYNIIS"
     gene            complement(12933..13070)
                     /locus_tag="L3M60_000915"
     CDS             complement(12933..13070)
                     /locus_tag="L3M60_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225609.1"
                     /translation="MEINYELIIIVMLFVILMSIQFTLNKIYLILKEIRQILMLKKNK
                     E"
     gene            complement(13148..13717)
                     /locus_tag="L3M60_000916"
     CDS             complement(13148..13717)
                     /locus_tag="L3M60_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBN6225610.1"
                     /translation="MKSILCQINKQPVKAKYTSDELECFLDTIEYNVGDKILLVGHIG
                     NLGKRLRGLGTSVTILENSNYEDVCYSLVYNENCNVVKGKLEFLPFDDNQFNKIIIVD
                     QFNVITDCEKASSEIQRVLKGNGEVILEDLNLKNLKVKLNYLKHKFCGSVAKIHYPHE
                     VFNIFSRLNFDGMIKESNKLKYIYVGKRR"
     gene            complement(13901..14536)
                     /locus_tag="L3M60_000917"
     CDS             complement(13901..14536)
                     /locus_tag="L3M60_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN6225611.1"
                     /translation="MIAVKDENNVVNLRGELDNKLEFSHEIFGEKFYNMKIKINRLSD
                     SFDILPMTVSERLLQDIDLDKQNLVNVVGQLRSYNKTLNNKNRLVLTVFVREIKSIDE
                     ENKDPNSIFLDGYVCKEPVYRKTPLGREITDLLVAINRPYNKSDYIPSIVWGRNAKFA
                     KNLKVGDRIQLWGRVQSREYEKKIDENNVVKKMAYEVSISKIKKLDENNNE"
     gene            complement(15202..16083)
                     /locus_tag="L3M60_000918"
     CDS             complement(15202..16083)
                     /locus_tag="L3M60_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBN6225612.1"
                     /translation="MVAILKALYVVFAAIFAVVFGKDVAKANKEGKLEEQSTPKVAVT
                     GFITDFFDTLGIGSFAPTIAMSKALKLNIPDKKMPGTLNVAHTIPVVTEAFIFTSIIP
                     VDGVTLVSMVVAAAVGSYIGAGIIAKMDERKIQLVMGVTLALTAILMLLGHPWVNVLP
                     GGGNELGLTGVKLVIGVIGNFILGALMTAGIGLYAPGMAMVYFLGMSAKVAFPIVMGS
                     CALLMPVASMKFIKEDAYTKKASVIIAICGLIGVFVAAYIVKTLPMDILKALVIVVIA
                     YTSATMIMAANKNKKTA"
     gene            complement(16203..17210)
                     /locus_tag="L3M60_000919"
     CDS             complement(16203..17210)
                     /locus_tag="L3M60_000919"
                     /EC_number="5.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase"
                     /protein_id="HBN6225613.1"
                     /translation="MKFSRSIQAIDSHTAGEATRIVVGGIPNIKGNSMPEKKEYLEEN
                     LDYLRTAIMLEPRGHNDMFGSVMTQPCCPDADFGIIFMDGGGYLNMCGHGTIGAMTAA
                     IETGVVPAVEPVTHVVMEAPAGIIRGDVTVVDGKAKEVSFLNVPAFLYKEGVEVDLPG
                     VGTVKFDISFGGSFFAIIHASQLGLKIEPQNAGKLTELAMKLRDIINEKIEIQHPTLA
                     HIKTVDLVEIYDEPTHPEATYKNVVIFGQGQVDRSPCGTGTSAKLATLHAKGELKVGE
                     KFVYESILGTLFKGEIVEETKVADFNAVVPKITGSAYITGFNHFVIDEEDPLKHGFIL
                     K"
     gene            complement(17230..17712)
                     /locus_tag="L3M60_000920"
     CDS             complement(17230..17712)
                     /locus_tag="L3M60_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBN6225614.1"
                     /translation="MSFVGISTSMKNTSLHIFRDPLLDLIDNDSDIDLLGVVVVGTSE
                     HNEWKTFLATRLGRWIESLRPDGVIITLDMAGNQHIDFTNAIAEFVKSDIPTVGLTIM
                     GVDGLVITNPYLDKATIIDYKKTTGYIETEVVGDNHMDEVDVKKALAFLKLKMRKDAK
                     "
     gene            complement(17729..18196)
                     /locus_tag="L3M60_000921"
     CDS             complement(17729..18196)
                     /locus_tag="L3M60_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase"
                     /protein_id="HBN6225615.1"
                     /translation="MGIGPSTKETSLHHFRDPLLDIVESDKDVDLLGVIVVGTPDGNE
                     NKTFVGQRTAAWLEAMRVDGAIVSSDGWGNSHVDYANTFEEIGKRDIPVVGVTFNGTQ
                     AKFVVSNQYMDTIVDMNKSKEGIETEVVGENNTNEIDAKKALAFLKLKMRKHG"
     gene            complement(18211..18969)
                     /gene="prdD"
                     /locus_tag="L3M60_000922"
     CDS             complement(18211..18969)
                     /gene="prdD"
                     /locus_tag="L3M60_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase cluster protein PrdD"
                     /protein_id="HBN6225616.1"
                     /translation="MEEKIIRRLVIKPFHMNEVNFGSKTSIKKDVLTIDLSSIDEIKE
                     REELITDIKVDIIKPGDYDREINTIMDIIPISTKVLGRLGEGITHTLTGVYVMLTGAD
                     EDGNQMHEFGSSEGILKEQMVFGRYGTPSVEDYIIHVDVTLKGGLPFERTLPLAAFRA
                     CDDFIQEIRASLKMEDGRNATQVREYFDKIRPNAKKVVIVKQIAGQGAMYDNQLFSKE
                     PSGFEGGTSIIDMGNVPMIISPNEYRDGALRAMT"
     gene            complement(19049..19774)
                     /gene="prdB"
                     /locus_tag="L3M60_000923"
     CDS             complement(19049..19774)
                     /gene="prdB"
                     /locus_tag="L3M60_000923"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(19322..19324),aa:Sec)
                     /transl_table=11
                     /product="D-proline reductase (dithiol) protein PrdB"
                     /protein_id="HBN6225617.1"
                     /translation="MSLTTVQGLQSEIFVPITPPPVWTPVTKELKDMTIALATAAGVH
                     LKSDKRFNLAGDTTFRAIPNTATVDEMMVSHGGYDNGDVNKDINCMFPIDRLHELAAE
                     GFIKGVAPMHYAFMGGGGNQHVFTEETGPAIAAKLKEEGVDGVVMTAGUGTCHRTAVI
                     VQRAIEEAGIPTIIIAALPPVVRQNGTPRAVAPLVPMGANAGEPHNIEMQTHILRDTL
                     EQLVAIPSAGKIVPLPYEYKAHV"
     gene            complement(19812..20096)
                     /locus_tag="L3M60_000924"
     CDS             complement(19812..20096)
                     /locus_tag="L3M60_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225618.1"
                     /translation="MDRLKYISSETFYEGIIVDIKGGGVTIDLKGRLGQFKIPNRMLI
                     TDYELKIGQEVGFMLSYPEVLSPEPNQEYVENIRREKEKQAEMQSKQEEK"
     gene            complement(20177..22057)
                     /gene="prdA"
                     /locus_tag="L3M60_000925"
     CDS             complement(20177..22057)
                     /gene="prdA"
                     /locus_tag="L3M60_000925"
                     /EC_number="1.21.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-proline reductase (dithiol) proprotein PrdA"
                     /protein_id="HBN6225619.1"
                     /translation="MSITLETAQAHANDPAVCCCRFEAGTIIAPENLEDPAIFADLED
                     SGLLTIPENGLTIGQVLGAKLKETLDALSPMTTDNVEGYKAGEAKEEVVEETVEEAAP
                     VSEAAVVPVSTGVAGETVKIHIGEGKNISLEIPLSVAGQAGVAAPVANVAAPVASAAA
                     EVAPKVEEKKLLRSLTKKHFKIDKVEFADETKIEGTTLYIRNAEEICKEANETQELVV
                     DMKLEIITPDKYETYSEAVLDIQPIATKEEGELGSGITRVIDGAVMVLTGTDEDGVQI
                     GEFGSSEGELNTTIMWGRPGAADKGEIFIKGQVTIKAGTNMERPGPLAAHRAFDYVTQ
                     EIREALKKVDNSLVVDEEVIEQYRREGKKKVVVIKEIMGQGAMHDNLILPVEPVGTLG
                     AQPNVDLGNMPVVLSPLEVLDGGIHALTCIGPASKEMSRHYWREPLVIRAMEDEEIDL
                     VGVVFVGSPQVNAEKFYVSKRLGMLVEAMEVDGAVVTTEGFGNNHIDFASHIEQIGMR
                     GIPVVGVSFSAVQGALVVGNKYMTHMVDNNKSKQGIENEILSNNTLAPEDAVRIMAML
                     KNAIEGVEVKAPERKWNPNVKLNNIEAIEKVTGEKIVLEENEQSLPMSKKRREIYEKD
                     EN"
     gene            complement(22486..24246)
                     /locus_tag="L3M60_000926"
     CDS             complement(22486..24246)
                     /locus_tag="L3M60_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBN6225620.1"
                     /translation="MFSIPEVKKVEEVMDTKFTTIDEDTRIEDAIKEMIKSNTKTLMV
                     IDSSGQLKGIISMTDIHNLYEMHKKYEGQPVKLIMKKDVIYVNEGLTLDECRDIMILK
                     NIGILPVLRDNKIIGVLKQEHIRDYLYMHLEDYGLTLKYIIGQIKEGICAINNEGVVI
                     LWNKFMEERYDIKSEDIVGRPMNEFLENTISERVLNSKVGMSDLYFTDKKENMYALVH
                     ANPIFYKEEFIGVVCTEVDVTEAKILALELEKVNDTLKYLKNEVKNLSKGSFDKILGK
                     SYKLEKSKAIAKQVARTNSSIFIWGESGTGKEVFARAIHDYSERKGQFIPVNCSAIPN
                     ELFESEFFGYESGAFTGASKKGRIGIFELAKDGTVFLDEIADLPLSMQAKLLRVLQEK
                     EIRRVGGDTTIKINPRIISATNKDLSKMVKAEKFREDLYYRLNVVEIKIPPLRERKED
                     IGLLVHSFLEEICKQNNKPVLTISKEVIDIFQNYRWKGNIRELKNTIENIVVLSQNSK
                     IDVDDVPSYMMDSTNNSTEEEEYPLDLTKATQKIEIKNITKALKMSNGNKAKAAKILN
                     IPRTTLYYKIDQYKINVSKI"
     gene            complement(24653..26767)
                     /locus_tag="L3M60_000927"
     CDS             complement(24653..26767)
                     /locus_tag="L3M60_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HBN6225621.1"
                     /translation="MNTIYVFSIRRILKTKIKKSSIISVVIAFSMVFTAFTPIVSYAD
                     EVIGNDTIINGEKPLEGQTPEGEKPLEGQIPEGEKPLEGQIPEGEKPLEGQTPEGEKP
                     LEGQTPEGEKPLEGQTPKGEKPLEGQTPEGEKPLEGQTPEGEKSLEEEIVIEEMSLKQ
                     DIDKILDMTLSQINKIVYNFWEDEENVKSDEQSEINQVFTSKDSFISLWYDKKAKVKN
                     TCLLKEIDGSDRPYHDLRFDDITKTLTFDYVIKGLVGASSKDNKYVIEGEDGEQTAFC
                     YNNHLRPPSSNGKSPYLPAEEFSGQENQNEDAVKSILYAGSEFDGFGYKQQFNLGGEE
                     NDIMTYSATQSAIWIILGQVDEQEKLKQYQGSINFCDFLIENAKNEEEKAEYQKKKEV
                     AINTKAYLEALLKAGREELKPNDTGKPSLSNGLTKINFEKNEDGTYETEAVALVGYSG
                     VVKLRLPDGVTAYDGDGNIIGTGEVEISTQQKFKLKSVGKPDAKANISAISYDYIFPK
                     AIQYYKAVIDLGQKDHSSSLPSSKQNLLSYTVEKKNGQEVNFNISLPTDDVVNPPVPP
                     TDDTVINPPVPPTDNTNGHKPKPSPPIDDTVINPPVPPTDDTVINPPVPPTDDVVLNP
                     PVPPTDDAVLNPPVPPTDDAVLNPPAPPVSDTVEKMPELSKYDTIVKSPKTGDETQLM
                     SYVFISIIAICGLAYQFKIKRN"
     regulatory      complement(27298..27383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(27786..29099)
                     /gene="prdC"
                     /locus_tag="L3M60_000928"
     CDS             complement(27786..29099)
                     /gene="prdC"
                     /locus_tag="L3M60_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline reductase-associated electron transfer
                     protein PrdC"
                     /protein_id="HBN6225622.1"
                     /translation="MNKLYSILLKQHVGGPDKPVVSVGDVVKKGTLIAEPTGLGANIY
                     ASVSGKISEINDQAIVIEADEVQDDTFEPLKGEGILDLIKEAGVVGMGGAGFPTHVKL
                     NIDLNGGTILANAAECEPLLAHNIKEIEERPEIVYQGIKYAMEVTNAGKGMLAIKSKH
                     PKAIEAFKKVIKPGDNIEVAELVDMYPMGEERAIVRDVLGKLLEPTQLPSEANAVVIN
                     VETLTRIVEAVEQKRPVISKNITVVGQLNSGKESIVFEDVPIGTTVGELIERAGGIKG
                     EYGEIILGGPFTGKATTLDAPITKTSGGIIVTMPFVNEKRKMGLLVCACGPNEERMRD
                     IATKMGVTDIVSVQKCKQAQEIKGALKCENPGHCPGQAQKCIEFKKAGAEVILIGNCT
                     DCSNTVMGSAPKLKLGVYHITDHVMRTVNHPLIRRIKGDVRLKIK"
     gene            complement(29489..30253)
                     /locus_tag="L3M60_000929"
     CDS             complement(29489..30253)
                     /locus_tag="L3M60_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6225623.1"
                     /translation="MKREVKRTKALIYSVLFFSLLLVLIFVFVYGIKYICSKSDNSKM
                     PIYKVDTNEKKIALSFDVAWGTDNMDDILKILDKHNVKATFFLVGSWVDDNEEIVKAI
                     DEKGHEIGNHSNTHANLKEISKEDITKEIETTSEKIYNITGKKTNLFRPPFGDVNNKA
                     MDICSDLGYKVIKWDVDSIDWKELGPNHVIEKVIKESRPGSIVLFHANINDVDNYLDT
                     IISRLKKDGYSLVKISDLLYKDNYVVDSNGVQKTKN"
     gene            30544..30747
                     /locus_tag="L3M60_000930"
     CDS             30544..30747
                     /locus_tag="L3M60_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBN6225624.1"
                     /translation="MSNSNRTVVPEAKAALNQMKLEIANEIGLSNYENIDKGNLTARE
                     NGYVGGYMTKKLVEMAERQMAGK"
     gene            30938..31474
                     /locus_tag="L3M60_000931"
     CDS             30938..31474
                     /locus_tag="L3M60_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4364 family protein"
                     /protein_id="HBN6225625.1"
                     /translation="MFENSSEELAYHKLLILYILEKIKMDLTNSQITQVVLETEMMNY
                     FSLQQLLSQLMESKFLTTYKDSDREYYALTQRGVESLEYFFNRIPTSVTEKIDKYIDS
                     NKENLLADTQVKSSFVKQSDNEFIVNLRVIENQSNLIDLNLNVSSEKQAKLICNNWKN
                     NASYMYAEVIDLLIRDIH"
     gene            complement(31478..32344)
                     /locus_tag="L3M60_000932"
     CDS             complement(31478..32344)
                     /locus_tag="L3M60_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBN6225626.1"
                     /translation="MKIYISNYLSKSISIVDYSTLELEKEIVLEDNIYPHHFCIEKEK
                     NLIYIPSSSNGILYVLDLSNDKIIDTVSIGGSLSQVVLSDNELFVANEDSNSIYVLDK
                     NTLNPIGIIGVDNMPHGFDFDRESKKLYVPCINSIVCIDTINKNIQKKINMDFRAWHI
                     ELDKQKKEMYISTLDGKLIIIDEVSMDIKKVLYEFLLPVEIRFNYSGKKVYVADLGYN
                     NVRILDYTTSRYIGNIEINGIPQGLEISKDEKLLFVSDTQENSVKVYETANNKLIKEI
                     KVGKEPTTIICL"
     gene            32492..32737
                     /locus_tag="L3M60_000933"
     CDS             32492..32737
                     /locus_tag="L3M60_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03905 family TSCPD domain-containing
                     protein"
                     /protein_id="HBN6225627.1"
                     /translation="MKVTFNPSGVCCREMIFEVDENNVIVDAEFIGGCNGNLSGLRSL
                     IIGQNALEVADKLNGIDCGGKGTSCPDQLSKAIRQSI"
     gene            complement(32857..34155)
                     /locus_tag="L3M60_000934"
     CDS             complement(32857..34155)
                     /locus_tag="L3M60_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBN6225628.1"
                     /translation="MKYEEALEYISQTNKFGIRLGLENIGKLLELLGNPQETLNIIHV
                     AGTNGKGSVCSFVSNILRECGYKVGLYTSPYLETFTERIRVNGQNIPQDDVARIIELI
                     KEKIEIMVREGYAYPTEFEVVTAMAFYYYSEQKVDFVALEVGLGGRYDATNIITKSLV
                     SVIVSISLDHTGILGDTIEKIAYEKAGIIKENGVVLVYDQTDEAKDVIKSVCKEKKAK
                     YIEVNFDDVNIKKSDINSQIYDCTVMKENYRDLEIKLIGEHQINNSILAISVIKYLKD
                     INKLANINEESIRKGLIKTKWPGRIEKIKENPIFIIDGAHNEDGAKSLAKALDKNFKD
                     RKLTLLIGMLEDKDIDSVLEVLLPHFNKVITTTPINPRAINSDILREKVLKYVDDVTS
                     KHEIEDAVNYTLETSSEDDIIISAGSLYMIGTVRTLVKKL"
     gene            complement(34288..35319)
                     /locus_tag="L3M60_000935"
     CDS             complement(34288..35319)
                     /locus_tag="L3M60_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBN6225629.1"
                     /translation="MENICDMNNKVKVAVLDTGIDKEHDYLKDNLVGGIAFECMHDYI
                     FISDKFDDEDGHGTACASIIKKEYEDVELFVIKILRKDGITNIKVLEEALRYLLDTNI
                     RLINLSLSVIGVESVKGLFEVCYELFRKGKIIVCSLANDFDLSYPAMFNNVIGVRGLT
                     LDVDDSFWYNKKYDVQCVMDSNSYISCDINNSYRLPPKCNSYLSAKLTGKIAKILSEK
                     PGITISDLNDKLESLAIRNHWNSCDFDECSRIVNFKLDLYDKENDLLIKVADVVRDCL
                     NIEDNNEKLFKCSLFNKEIGLVYDNCFNLLKKLEYRFDVKFNYMDISKYDLVSIYTLT
                     ELVERYKNL"
BASE COUNT        10962 a         6298 c         4400 g        13760 t
ORIGIN      
        1 ttactaaaat caaataaaac aatgttttta taatttaaaa ttggggtgaa tttatatgaa
       61 aaaagtaaat gtagcaatag ttggtgctac tggaatggta ggtagaacat ttttaaaagt
      121 attagaagaa agaaattttc caatagaaaa tctattttta ttctcttctt ctaaatcagc
      181 tggttctaaa gtaatgtttt gtggtaaaga atatatagta gaagaattaa aggaaacttc
      241 atttactgat agaggcatac aaatagcatt attctctgct ggtggaagta ttagtgaaaa
      301 gtatgctcca atagctgctt cgaatggaat cttagtggta gataactcta gccaatggag
      361 aatgaatcca gatgtacctt tagtagttcc tgaagttaat ccagaagcca taaaaaatca
      421 taagggtata atagctaatc ctaactgctc tactatacaa gctatggtcc ctcttaaagc
      481 tcttgatgat aagtacaaaa ttaagagagt tgtttactct acttaccaag ctgtatctgg
      541 ttctggtgtt aaaggggttg aagatttaga gaaaggaatg aacggtaatc ctactggatt
      601 ctaccctcat caaatctctt acaactgtct tcctcatata gattcattca ctgaaaatgg
      661 atacactaaa gaagagatga aaatggtaaa tgaaactatg aaaatacttg ataactatga
      721 tttaaaaata actgcaacta ctgtaagagt tccagtaaaa aatggtcata gtgaatctat
      781 aaatgttgag tttaacaatc catttgaatt agatgattta gtaaaaacac ttgaagaagc
      841 tccaggtgtt gtagtagttg ataatcctgc taaaaacgaa tatccaactg ctgtagaatt
      901 tgatgataga gatgaagtat ttgttggaag aataagaaga gattttagtg ttgataatgg
      961 tgtaaacctt tgggttgttg ctgacaatat aagaaaaggt gctgcaacta acgcaattca
     1021 aatagctgaa atggctttat catacgattt agtataatat acaaacttgt ataaatatat
     1081 aattttgtaa aatattaata gtaactcaaa aacaagcatt agaagtaact gtaatataaa
     1141 acacaaaaat aaaaaagagg ggattgattt tatatgatat ttaaaggttc tgctgttgct
     1201 ctagttacac catttacaaa ggataacaaa gttgattttg acaaattagg tgaattagtt
     1261 gagtaccaaa tagctaatgg tactgatgct atagtatctt gtggtactac tggtgaagct
     1321 aatactatga ctgatgaaga acaacttgct actattaagt atgttgttga aaaagttaat
     1381 aaaagagttc ctgttatagc tggaagtggt tctaatgata ctatgcactc agttaactta
     1441 agtcaagaag ctgaaaaatt aggagtagat gctctattaa taataactcc ttattacaat
     1501 aaagctaata aagctggact taagcgtcat tttgaaacaa tagctaacag tgttaaactt
     1561 ccaattatac tttacaatgt ccctggaaga acttgtgtaa atataagtcc tagccttata
     1621 gttgaacttg ctaaaattga taacatagtt gctgtaaaag aagctagtgg agacctagga
     1681 caggttgcag aaatagctag tttagttcca gatgactttg ctatctactc tggtaatgat
     1741 gatactatac ttcctttatt atctttagga ggtcaaggag ttatatcagt acttgctaat
     1801 gtttgccctc aagaaactca cgatttggta gctaaattct ttgaaggcga tatagaaggt
     1861 tctagaaaat tacaacttgg tatggatgct ctaattgcag ctttatttat agaagtaaat
     1921 cctataccag ttaagactgc aatgaattta ttgggattca atgttggaga tttaagatta
     1981 cctctagcag aaatggaccc agcaaattta gaagtgttaa agaaagaatt agttaacttt
     2041 ggactaaaag ttaatgcata gtaaagatta atgcatggta aaatgataag tttggtcaaa
     2101 agttagccct taatcatagg aggaaattat gttaaaagtt atagcaactg gttatgatgg
     2161 aaaaatgggt aagattttag ccgatactat aagagaagat agtgaattag aattggtttg
     2221 tgttgcagct agaggtctgg atagttatga agacgacctt aaaatctatg aagatatgag
     2281 cactatagtt gaagaagcag atgtagttat agatttttct catcactcta atttagataa
     2341 tatcttatct tacgttttaa agactaagac acctttagta attgctacta ctggatataa
     2401 tgatgaagaa atgaacaaaa tacatgaagc agctaaagtt attcctgtat tccaatcata
     2461 taatatgtct cttggtgtaa atgtattagt aaaattggtt aaagatgcag ctaaattact
     2521 agaaggattt gatatagaaa taatagaaaa acatcacaac agaaaagtag atgccccaag
     2581 tggcacagct gttatgattg ctaacgctat aaaagaagtt ttgccaaacg ttgaaaataa
     2641 ttatggtaga tatggccgta gtgcgaaaag acaaccaaat gaagttggta ttcatgctat
     2701 aagaggtgga aatatcgttg gcgaacatga tgctttattt gctggagaca atgaggttct
     2761 tacaataagc catcaagctc aatctcgtgg tatcttcgca agtggttcta tagctgctgc
     2821 caaatattta gttaaacaaa aaccaggatt ttataatatg gacagcatgt tgggataata
     2881 atataagtat aataatgcac aatataaatc aaacatatat aatagataaa tttacttaat
     2941 actatgtcta tataataaaa actaattaaa atataaataa taagaagtaa atatatacta
     3001 aaattgacaa acaagtatat aataaaagaa atataatgaa atacaaggag aattaacaaa
     3061 tggtaaattt aaacgatgct tatgaaatag caaggtatat aaaagaagtt aaaaagaaaa
     3121 cacctgttaa ggtatatgta aatactaact ctttacattt ccaatcaact gataaattta
     3181 aggtatttgg atgcaatggt tcttatatat ttgtaggtga ctatgaagat ataatggaag
     3241 tattagataa acatgggaat gacataatag atactcatgt agaatatgat agaagaaatt
     3301 ctgctattcc tctaaaaaat actttaagtg aacatgcaag aattgaacca ggtgcaataa
     3361 taagagatat ggttactata gaaaaaaatg ctgttgtaat gatgggagct gttataaata
     3421 taggagctgt tattggtgaa ggttctatgg tagatatgaa tgctgttatt ggagctagag
     3481 gaactcttgg taaaaatgtt caccttggtg ctggtgctgt tgttgctggt gttttagagc
     3541 caccttctgc tactcctgtt atagttgaag atgatgtttt aataggagct aatgcagtta
     3601 ttcttgaagg tgttagaatc ggtaaaggtg ctgttgttgc agctggtgct gttgtaacta
     3661 ctgatgtaga agctggtgct gttgttgctg gttctcctgc taaagttata aaaatgaaag
     3721 acgaaaaaac ggctgacaag actaaactta tggaagacct tagaaattta gattaattaa
     3781 atgacaatat attaattgaa aagattaatt aaataataat atactaattt aaaaattaat
     3841 taaaaggtgt ctctatatga atttaattca ttagagacac ccttttttat aatattttta
     3901 tcttttattt atttttataa ttctcttatt cattctcata tcatttttat ttctttctga
     3961 taagtatttt tatttccttc aatttttcaa tatatcaatg aaacaaacta gcgaacttta
     4021 tataaaccaa taaagtaaac tactaaactt tatataattt aataaataga actgctgttt
     4081 actactggtt gtgcatcttt attgccacaa agaacaacta acagattact taccatagct
     4141 gctttacgtt cttcatccaa agttacaaca ttattttcac ttaaattagt caatgccatc
     4201 tctaccatac ttacagcccc ttcaacaatc tttttacgtg ctgctattat agcctctgct
     4261 tgttgacgtt gtaacattgc tgctgcaatc tctggagcat aagaaagatg agtaattctc
     4321 acttcgcata cttcaattcc tgcaatatca acccttgatt gtaattcatc ttttaatcta
     4381 tctgctattt cctgactact accacgaaga gatttttcat ctccatcttc agacacatca
     4441 tatggataaa gtcttgaaac attacgaata gtagaatcgc actgaataga taaaaatgtg
     4501 ttataattat ccacattaaa tactgccttt gttgcatcta taaccttcca aattacaaca
     4561 actcctatta taattggatt tcccaataaa tcattaactt tctgtttttc attattaaga
     4621 gtcattgtct ttaaagatac tttcttacct cttgaattag ttgatatatc tactttttcg
     4681 tcagatgaag attttgatac attaattctt gatgctgctg ggtttattgc tgaacaaaat
     4741 ggattcaccc aatagtaccc ttcttttttt atagttccat aataattacc aaacaaaact
     4801 aaaactatag cctctttagg attaatcatt ttcagcccaa agaatagaat aaaatctact
     4861 actataaata taagcccaaa aattaacata agtgaaccta ttccagtaaa tccactatca
     4921 agttttacta tcccaaaaac cataatacct attgaaacta ctaaaaataa cacaatcatt
     4981 atcaaagctg ctactccatt aaatactttt gcttctttct cttgataagt tttttcttct
     5041 tttaacatat ctatcactcc catctcagta tattaagtta agatgtttct aatttaataa
     5101 taacactttt aacacttaaa ttgttgaatt tgaaattaat actataatgt atttatatat
     5161 ttttcataca tctttataca tacatgtgct tagttatttt aatgtattaa tattgatttt
     5221 taaataaatt atacgatata tgaatgtggt atctcaaaat gaactttttt gattcatgag
     5281 gttctctttt ttgtatgaaa ccaaatatat ttttatcatt tcaacagtga aaaaaagctt
     5341 atctatactt tgacaaagct acttcaattt tttaatgtaa gataaattgt aaacctatta
     5401 tcactaatat tattttatat agataatatt agtgataaaa taaacttata gaaatacaac
     5461 aaatttttat taattttata ggaggaattt atgttgaaag ttataattag cggctataat
     5521 ggaacaatgg gaagagtgtt agcagaatgt gtaaaaaaag ataatgaatt aaagttggtt
     5581 tgtggtgtag ctaggtctgc tgatgaaaaa agtcatcata gtgatacaaa gctttacaaa
     5641 acaatgactg atgtaactga aaaagctgat gtcataatag acttttcaca tcatgagact
     5701 ttagagccaa tattgtcata cgctctaaaa aataatacgc ctgtagtatt agctactact
     5761 ggatatgatg aaagtgattt aagtaaaata gaggaagcat caaaaaatat tcctatatta
     5821 ttatcatcaa atacatctct tggagttaat atattaataa aattagttaa ggacgcttca
     5881 aagatgttag aaggttttga tattgaaatc atagaaaagc atcacaatag aaaagaagac
     5941 gctcctagtg ggactgctac tatgatagct aatgcaatta aagaagtctt gcctgaaagc
     6001 acaaatacac atgggagata tggtagggat tgtaaaagaa aagcaaatga aattggaata
     6061 cactccataa gaggaggcaa tataatcagt gaccatgatg ttatatttgc aggtgataat
     6121 gaagttctta ctttaagcca tcattctcaa tctgatgaag tatttgcaaa cggttctata
     6181 gttgcagcta aatttttagt tggtaagcaa tctggacttt ataaaatgtc agatatatta
     6241 ggataaattt tgtacataat aaatctcaat ctatttggat attcatattc atcaatttga
     6301 atatctaatt gattgagatt tctactctaa aagtttataa attttatacc tactttttaa
     6361 tatagtcatt ctgctaaaga ttatattatt ttagtatata gaactatatt gttcattatt
     6421 ttacagtatt ttcaccatat agacttatta aaaattatat tatttattag tatataaaat
     6481 catattgttt attattttat agtcctttca ttatataaac tattctattc tataaactga
     6541 tacatatcca gctggagaag tgcctgtcgt gcctgaacca aatgtaaaac ttaaagtaac
     6601 agctgctgct ctagtatcaa ctaaaaaact taaacttaca cttacatctg atgaagtaag
     6661 cgtatttact gcttttgcaa taaaattatt ttgagctact ccattgaaat tagtagtaat
     6721 tgacataaat cctgcaagtg aaagtgtggc acgtatacca aaagatacat tatatattcc
     6781 tccaactgca agatttatag tactacttgt tggaaaagtt atattagttc catttataaa
     6841 tgatgaatta aatgtcacta atgtattatt ccctaaactt gaagaagata ctgtaaattg
     6901 tgcattattt gctgctaaca ctccagttgc tccagtagct cctgttggac cagttgctcc
     6961 gtttgctcct gttgctcctg tatttcctgt tgctccagtc actccatttg ctccagttgg
     7021 acctgttgga cctattggac ctgctactcc atttgctccg gttgctccta ctgctccggt
     7081 tgggcctgtt gctccatttg ctccggttgc tcctgtcgct ccggttgctc ctgctactcc
     7141 atttgctccc gttggacctg ttggacctat tggacctgct actcccgttg ctccggttgc
     7201 tcctactgct ccggttggac ctgttgctcc atttgcccct gtcgctcctg tcgctccggt
     7261 tggacctact gctccatctg ctccagttgg gcctgttgct cctactgttc cggttggacc
     7321 tattgcccca gctactccgg ttgctcccgt tgggcctgtt ggacctacca aaccatttgc
     7381 tccggttgct cccgttgggc ctgttgggcc taccaaacca tttgctccag ttgctccggt
     7441 tgctcccgtt gggcctgttg gacctattac tccatctgga cctgttgctc ctactgctcc
     7501 tgttggacct acctctccat ctgctccagt tggacctgtt gctcctattg gacctgttgc
     7561 ccctgtcact ccatttgctc cggttgctcc tactgctccg gttgggcctg ttgctcctgt
     7621 acttcctgtt ggacctgtag aacctgttac tccatttgct cccgttgctc ctgtatttcc
     7681 tgttggacct gttattccat ttgctccagt tgcccctgtt gctccggtta gtccagttgg
     7741 acctgttgct ccggttgctc ccgtatttcc tgttggacct gttattccat ttgccccggt
     7801 tgctcctgta tttcctgttg gacctgtaga acctgttatt ccatttgctc ccgttgctcc
     7861 tttgtttcct gttggacctg ttattccatt tgctcccgtt gctcccgtat ttcctgttgg
     7921 acctgttatt ccatttgctc ccgttgctcc cgttgctccg gttggacctg tagaacctgt
     7981 tgctccattt ggtccagtcg ctcccatatt tcctgttgga cctgttattc cattcgctcc
     8041 tgttgctcct gtgtttcctg ttggacctgt tattccattt gctcccgttg ctccggttgc
     8101 acctctaggt ccagtcggac ccgttggacc agtagcacct gttggacctg ttggacctgt
     8161 tggacctaca tcttgatata aatttggacc tgaaatatca ctcatataat tatcctccct
     8221 ttttaaagtt agagtaatac tctataaata ttgagtatta ctctatataa ttatatgaat
     8281 cgacaatttc ttatgtgata atgtatctat ttacttatat caacatcaat tcttttattc
     8341 ttagatgtca tcacattttt ataaaatatt ttacaattat aaatacaatt aaaaaattca
     8401 tttcccacca atcatttgaa atttttttat tatgttataa aaaagatata gttgaactgc
     8461 acaagattaa tttaatgcta ctttaattgc tttttaaata aaaaaacctc aacacgatta
     8521 ataaaaatca tattaaggca tccacaaaat catataattg cttatctatc ttcatcagtg
     8581 acattgaata tataaggaac atttctataa taatcgccaa aatcaaaccc atatccaacc
     8641 acaaacttat cttctatttc aaatccacaa taatctggca ctaaatcgac ttttcttcta
     8701 cttggtttat ccaaaagaac acagcatttt aaactagcag gatttttagc ttttagatgt
     8761 tttaacacaa aatccatagt aagtgcagaa tcagtaatat catctacaac taaaacatca
     8821 tatcctgcta tatcagtagt aacatctgaa actactttaa cctttccagt actttcttca
     8881 ttatttccat aactagatgt agtcataaaa ttgactctaa gtggtaaatt tatatttcta
     8941 actaagtctg cacaaaatat aaaactacct tttaatagag aaactaccat aagattttta
     9001 tctttataat ctttttctat aattgctcct aattctttta atttatcttt aattttttct
     9061 tctgaataca acacttccca tttttttgtt tctatatcca taaaaagacc tccaattgta
     9121 ataatccttc ttaattttat aagagattgc tcactttttc aatactctta ttcaatatta
     9181 gccaatatta ttgagttttt atgttcattc aacaattatt aacaaggtat cttatatttt
     9241 aagtagttca tatatccata ctaaattaca tactatatat tttagacaaa aaaagaaaaa
     9301 acttgcacta acaagttttt tctattatac ataaatttat acataatcat attttattag
     9361 agggtactaa ttaaatttat ttatgctgtt tttgttattc ttcataatac aaaaccatat
     9421 tataaagtac tagtcaactt tattcatatc attcattaca tttaagatta tttcatctgt
     9481 tatagacata cctttgtcac ttactctacc taagttttgt atactttgct caacacttga
     9541 accaactata ccatttagtg gtggtacaaa acaatcttgc ttggctatta tagcactttg
     9601 tatagctgca gatgcagcag aagataattt tagtgcacaa ccagctttcg caccatcaca
     9661 tatcattcca cttaagtcag ctatcatatt ttctatagca ccttctattt gcttttcagt
     9721 accattcata agccaagaga taccagcagt tgctccagta gatgcagcaa caccacatcc
     9781 acatacagca gataatcttc ctgtataatt cttaatataa ccagttacta aatgagatat
     9841 agctaaagct tttgctagtc tttcatcaga ttgtgggaat tttttattgt aagcaacgat
     9901 tggaagaatt gctgttaatc cgtggtttcc acttccattt gaactcataa caggcatttt
     9961 tacccctgcc attcttgcat ctgaagctcc agcagttaac atcattgcat aatttataac
    10021 atcattgcct aaaagacctt cttctataga ctttttaatt ccatatccga caccaatacc
    10081 agttttttgt tttaatccat attcagccat ttcttcattc atttttactc catctagtaa
    10141 aaattctata tctttaaaat caatgccttc tacatttttt attaattctt gtatagttac
    10201 tgtatccatt aaactttcaa ctttttctgc tgaatcattt gaagctgata cttttggttc
    10261 attgtctaat aatacttctc catctttttc taagaacgta aaattatcat gtttagttct
    10321 tattctaact ttagcaatat gattttttcc ttttaataca acttctataa acactttctc
    10381 tcttgtatca gcaggtgtta ttgatagagg agtattatcc atataatcct cagctatttt
    10441 aacttcctct tttgttagag tctctaacac acttaatccg ttttcagaat gtccacctac
    10501 tatacctaga gctgctgcga tttttaaccc taatctttca gtacctggta tacctacgca
    10561 cataccattt ttatagatac tagggctaac taacattcgg ttttcaacta tttcttctcc
    10621 taataattcc tttgcctttg cacatgctag agctaaagct actggttcag tacatcctac
    10681 tgctggctta acttctgcct ttaacatttc cactaattct tttcttatat ctctcattat
    10741 atataaacct cccaattaag tttagattta gtactcaaat aattaattaa ataatttctt
    10801 atctatatgt atagcaaaat acatgccata ttttttatat ctgtttatat ttttaatttt
    10861 atattctata gccattggta tgactacttc tatttatgca ttttttttat ttgcatagga
    10921 tatttatatt ctttaaaatg gagaaacata tgacaatttc gcaaaaagac aaaaaaaagt
    10981 ctcaaaatga gattctatat cattttgaga cgactcaaac atattatttt ttaacttata
    11041 aaaactattt tttaattcat aaaaaagatt atgcaaaaca cttacaatag aaatttaaat
    11101 ccttctttaa agttatacat ttagaaaaat ttaaatttta tacagttttt tattatatta
    11161 ctatgaaatt atattatatt tttcaatttt tctatataat gtagctcttg atattccaag
    11221 tgctttagct gttaattctt tatctttttt atagtctttg tatttattta aagcttttat
    11281 tatctcaatt ttctctagtt catcaagtgg aattatccta tcgcttctaa cttccaactc
    11341 ttctacagag gtattcttaa ttttttgagg aattacattc attgtgatta ttgaagagga
    11401 ggacatattc acactatatt caataatatt ctgcaactct cttatattcc ctggccactt
    11461 atattccatc atagcatcca caactctgct atctatgtca ataacatttt tatctaattt
    11521 ttttgaatac tctttaacca tatgtttgat tataagtggg atatcttctt ttctctcttt
    11581 taaacttggt aattttatag gtataacatt taatctataa tataagtctt ctctaaaagt
    11641 tccattttga accatttttt ctaaatcttt atttgttgca gcgataattc ttacatctat
    11701 tgaaacattt gatttacttc caattttatt gagttccttt tcttgtaata ctctcaataa
    11761 ctttgactgt aaatgcaaac tcatatctcc tatttcatct aaaaatatac ttcctttatg
    11821 agctacttca aacattccaa gctttccacc ttttttagct ccagtaaaag ctccttcttc
    11881 atatccaaat aattcactct ctaataaatt gtctggtata gcagcacaat ttacagctat
    11941 aaaaggatta tctaccctat tgctatggtt gtgtatggct cttgcaaata gctccttacc
    12001 tgttccactt tcaccagtaa taagcactgt tgatgttgat ttagaagcta ttaaagcctc
    12061 ttttttagaa tttcttatag ctacactttc cccaattata ttttccaatt ttatcctata
    12121 atctttattc atcttattgt agttttttat agcatctctt ttatctataa aatcaataac
    12181 atatcccttt aactcatttt ttaagactat tttattgatg ttatatattc cctttgaatc
    12241 atatccgtat cttttataaa aaaaactaca ggatttatcc ttcttaaagt tatttataga
    12301 aggtttcttt ataaaatcta attcgacaaa tatgttttta ccagttatat tatcttttat
    12361 attgaaaagc tctttaaatt tcgaattata tttatcaatc tttccgtcta catcaataga
    12421 cacaacagct ttgtccatat tgtttaacag tgtctctaat tttttctttt ctaattcaag
    12481 ctcatacgtt ttaatttgag cttttaattt attagatatt aagtctgcca ttttgcctaa
    12541 aaagtttata agaccacttt tattattttc tataatctgt gcttgttcct ttgtaaaggc
    12601 tataaggcct ataacaccat gagagactcc atcacatata atgggacaac acacctctgc
    12661 aaactctaca caagccccac ttttatagca ttccctacat atatcactac tctttgggtc
    12721 atcaattagt attgtaatct gctctgtaag tgatttttta aaagccgtat atttatctac
    12781 tttctcacct attttgtcta tatatactcc tgtacctgca atcctaaata agtccttatt
    12841 tactatagtt acatctatat tgagtactga tactatagct tcagctatat tttgaatatc
    12901 agattttata tattctaaac ctatcataat catcattcct tatttttctt taacataagt
    12961 atttgtctta tttctttaag aatcaagtat atcttattta aagtaaattg gattgacatt
    13021 aggattacaa agagcataac aattattatt aactcataat ttatttccat ctaaacatct
    13081 ccttataaca aaagactagg cttaacctag tcttttatat tatatccata tttatatttt
    13141 aactcattta tcttcttttt cccacataaa tatattttaa tttatttgac tctttaatca
    13201 taccatcaaa gtttaatctt gaaaatatat tgaacacttc atgtggatag tgaatctttg
    13261 caacagaacc acaaaattta tgtttaagat aatttaactt aacttttaaa ttctttaagt
    13321 ttaaatcctc taatataacc tctccattgc cctttagtac tctttgtatt tcagatgaag
    13381 ctttttcaca atctgtaata acattaaatt ggtcaactat tattatcttg ttgaactgat
    13441 tatcatcaaa cggtagaaac tctaacttac ctttaactac attgcaattt tcattataca
    13501 ctagagaata acatacatct tcatagttac tattctccag tatagttaca gaagttccaa
    13561 gacctctcaa tctcttgcct aggtttccta tatgaccaac aagcaatatt ttatcaccta
    13621 cattatactc aattgtatcc aaaaaacatt ctaattcatc agatgtatat ttagctttaa
    13681 ctggctgttt atttatctga cataatatgc ttttcacaat gcttcacctc ttcatttcct
    13741 caattataaa ttatgtacag attacattat aataaatggc gagcaactat tcaagagata
    13801 tttcattagt atttttttaa acaatcttta tatattttaa tatgatatta taccaaactt
    13861 attaggattt tattcataaa agtcagtcta ctttttatgt ttattcattg ttattttcat
    13921 ccaatttttt tattttcgat atagatactt catatgccat ttttttaacc acattatttt
    13981 catctatttt tttctcatat tctctacttt gaaccctacc ccaaagctgt atcctatcac
    14041 caacctttag attctttgca aacttagcat ttcgtcccca aactattgat ggtatataat
    14101 cagatttatt ataaggtctg tttatagcaa ctaataaatc ggttatttcc cttcctaaag
    14161 gtgtcttcct atatacaggt tccttacata catatccatc taaaaatata ctattagggt
    14221 ctttattttc ctcgtctatg ctttttattt ctctgacaaa cactgtaagc actaatctat
    14281 ttttattatt taatgtttta ttatatgatc ttaattgacc tacgacattg actaaatttt
    14341 gtttatcaag gtcaatatct tggagaagtc tttctgatac agtcattgga agtatatcaa
    14401 aagaatcgct taatctgttt atttttattt tcatattata aaatttttct ccaaatatct
    14461 catggctaaa ttctagctta ttgtctaact cccctcttaa gtttactacg ttattctcat
    14521 ctttaacagc tatcatattt tatccctcca caattgttaa ttcttatatt tctggtatca
    14581 gcttatataa taaatatatt tataagggat gtattttatt acaaatcttt gattgctgtt
    14641 taaaagtttt tatttggtta taaatttcat ttaatatttc acttttataa ttataaaatt
    14701 actatctttt tgtaccatat atactaatta aactatctat ttcagttttg tattaattat
    14761 aaaattactg ttatacaatt aataagtcat atataactta aatttatttg ctctatataa
    14821 gcaatttcaa ggaattaaac ttatttaaat aattcagata agtattaagt cattataaac
    14881 ttatttttct taactataag ttatattaga cttattacgg cttatatttt tattaatctg
    14941 tattaatata tgaacattta ttaaacttat tgattataaa atttaaatat ttagctttaa
    15001 attatgcttt attatttaaa aatacaacgt tttttacatt aaatctacta aattactata
    15061 tatattacat aattggcatg ttaattgctt tatatattag taaataaaaa aactgctcta
    15121 aaatattaat tcaatatttt aaagcagttt ataatcttta ttattagctg taatatttga
    15181 taaacactaa gtagtcttta actaagcagt ctttttgttt ttatttgcag ccattatcat
    15241 tgtagcagaa gtataagcta taactactat tactaatgct tttaatatat ccattggtag
    15301 agtttttact atatatgcag ctacgaaaac tcctattaaa ccacatatag ctattataac
    15361 tgatgctttt ttagtatatg cgtcttcttt tatgaatttc atacttgcaa ctggcattaa
    15421 taatgcacaa gaacccataa ctattgggaa tgcaactttt gcagacattc ctaagaaata
    15481 aaccattgcc atacctggag catatagacc tataccagct gtcattaaag ctcctagtat
    15541 aaagttacct attacaccta ttactaattt aacaccagta agacctaatt catttcctcc
    15601 acctggtaat acgttaaccc aaggatgacc taataacatt aatattgctg ttaatgctaa
    15661 agttacaccc ataacaagtt gtatttttct ctcgtccatc ttagctatta ttcctgcacc
    15721 tatgtatgaa ccaactgctg ctgccacaac catagatact aaagtaacac catcaactgg
    15781 tatgattgat gtaaatatga atgcttctgt aacaactgga atagtatgtg ctacgtttaa
    15841 tgtaccaggc atttttttat ctggtatatt tagctttaaa gcttttgaca ttgctattgt
    15901 tggagcaaat gaccctattc ctaatgtatc aaagaagtct gttataaatc ctgttacagc
    15961 aacttttgga gtactttgtt cttctagttt cccctctttg tttgctttag ctacgtcttt
    16021 accaaacaca actgcaaaga ttgctgcgaa tactacgtat aatgccttta agattgctac
    16081 cataatccat cctcctattt ttttattttt tcatatattc tgaatcttat agcctaaaag
    16141 ctatagaata gtagccgata agtggctact attctatttt aatttcctaa tttatttatt
    16201 acttatttaa gaataaatcc atgtttaagt ggatcttctt catctattac aaaatgatta
    16261 aatccagtta tataagcaga accagttatt ttaggtacta cagcattgaa atctgcaact
    16321 ttagtttctt caactatttc acctttgaat aaagttccta atatactttc atatacgaat
    16381 ttttctccta cttttaattc acctttagca tgtaaagtag ctaatttagc acttgttcca
    16441 gttccacatg gagatctgtc aacttgacct tgaccaaata taactacgtt tttgtaagta
    16501 gcttctggat gagttggctc atcatatatt tcaactaagt ctacagtttt tatatgagct
    16561 aaagttggat gttgtatttc tattttctca tttattatat ctctaagttt catagctagt
    16621 tcagttaatt taccagcatt ttgaggttcg atttttaaac ctaattgact tgcatgtatt
    16681 atagcaaaga agcttccacc aaatgatatg tcaaatttaa cagttccaac acctggtaag
    16741 tcaacttcaa caccttcttt atataagaaa gctggtacgt ttaagaatga tacttcttta
    16801 gcttttccat ctacaactgt aacgtctcct cttattattc ctgctggagc ttccataact
    16861 acatgagtta caggctctac tgctggaact acacctgttt ctatagctgc tgtcatagct
    16921 cctattgtac catggccaca catgttaagg tatccgccac cgtccatgaa gattattcca
    16981 aagtcagcat ctgggcaaca aggttgagtc attacagaac caaacatatc attatgtcct
    17041 cttggctcta acattatagc agttcttaaa taatctagat tttcttctaa atattctttt
    17101 ttctcaggca tagaatttcc ttttatgtta ggtattccac ctacaactat tctagttgct
    17161 tcacctgctg tatgagagtc tatagcttgt atacttctgc taaatttcat atcaaattcc
    17221 cccttaggtt tattttgcgt ctttacgcat ttttaatttt aggaaagcca atgctttttt
    17281 aacatcaact tcgtccatat gattgtctcc aactacttct gtttctatgt agcctgttgt
    17341 ctttttataa tcaattatgg tagctttatc taaatatgga ttggtaatta ctaatccatc
    17401 tactcccata atagtaagac ctactgtagg tatatctgat tttacaaact ctgctatagc
    17461 atttgtaaaa tcaatatgct ggtttcctgc catatctaaa gttataatca ctccatcagg
    17521 tcgcaatgat tcaatccatc ttccaagtct agttgctaaa aatgttttcc attcattgtg
    17581 ctctgatgtc ccaactacaa ccactcctaa caagtctatg tcagaatcat tatctatcaa
    17641 atctagtaat gggtctctaa atatatgaag tgatgtgttt ttcattgatg tagaaattcc
    17701 cacaaaactc attccttact taaatttttt acccatgttt tctcatttta agttttaaaa
    17761 atgccaaagc ttttttagca tctatttcat ttgtgttgtt ttctccaaca acttctgtct
    17821 caataccttc ttttgatttg ttcatatcaa ctatggtatc catatattga ttgcttacaa
    17881 caaacttagc ttgggtacca ttaaatgtaa ctccaactac aggtatatct ctttttccta
    17941 tttcttcaaa tgtgttagcg tagtcaacat gtgagtttcc ccaaccatct gatgaaacta
    18001 tagctccatc cactctcatg gcttccagcc aagcggccgt tctttgtccc acaaaggttt
    18061 tattttcatt tccatctgga gttcctacta ctattactcc aagtaaatct acatccttat
    18121 cactttcaac tatgtctaaa agtgggtctc taaagtgatg aagagatgtt tcttttgttg
    18181 aaggtcctat tcccataata tccacctctc ttaagtcata gctctaaggg caccatctct
    18241 gtattcatta ggagatataa tcataggtac atttcccata tctataattg atgttcctcc
    18301 ttcaaatcca ctaggttcct ttgagaataa ctgattgtca tacatagcac cttgacctgc
    18361 tatttgtttt actatgacaa ccttttttgc atttggtcga attttgtcaa aatattcacg
    18421 aacttgagta gcatttcttc cgtcttccat tttcaaagac gctcttattt cttgtataaa
    18481 atcatcacat gctctgaatg ctgcaagtgg aagtgttctt tcaaaaggta aaccaccctt
    18541 taaagttaca tctacatgga ttatataatc ttcaactgat ggagttccat atctcccaaa
    18601 taccatttgt tcttttaaaa ttccttctga agaaccaaat tcatgcattt gatttccatc
    18661 ttcatctgca ccagttaaca ttacatatac accagttaaa gtatgtgtaa ttccctctcc
    18721 cagtctgcct aaaacttttg ttgatattgg tataatgtcc ataatagtat taatttctct
    18781 gtcataatct ccaggtttta taatgtctac ttttatgtct gttattaact cttctctttc
    18841 ttttatttca tctatacttg ataaatctat tgtaagtaca tctttcttta tagaagtttt
    18901 tgaaccgaaa ttaacttcat tcatatgaaa cggttttata acaagtcttc ttattatttt
    18961 ttcttccata atatccacct ttgttcttaa aacccctctc ctaactgtgt aggagaaggg
    19021 ttctatattt tcaaatataa attacatttt aaacgtgagc tttatattcg tatggtaatg
    19081 gaactatctt accagcagat ggtattgcaa ctaattgctc taatgtatct cttaatatat
    19141 gtgtttgcat ttctatgtta tgtggttcac cagcattagc acccattgga actaatggag
    19201 caactgctct aggagttccg ttttgtctaa ctactggagg aagagctgcg attattattg
    19261 taggtattcc agcttcctct attgctctct gcacgatcac ggcagttcta tggcaagtac
    19321 ctcagccagc tgtcataact acaccgtcta ctccctcttc cttaagttta gcagcgatag
    19381 caggaccagt ttcttcagtg aagacatgtt ggtttcctcc accacccatg aatgcatagt
    19441 gcataggagc tactcctttg ataaatcctt ctgcagctaa ttcatgtaat ctatctatag
    19501 ggaacataca gtttatatct ttatttacat ctccattgtc atatccacca tgtgatacca
    19561 tcatttcatc aactgtagct gtattaggta tagctctaaa tgtagtatct cctgctagat
    19621 taaatctctt gtcagatttt aaatgaacac cagctgctgt agctaaagct atagtcatat
    19681 cttttaattc tttagttaca ggagtccaaa ctggaggagg tgttattgga acgaatattt
    19741 cagattgaag tccttgtact gttgtaaggc tcatatattt cctctccctt ctattttcaa
    19801 acgattattt attatttttc ttcttgtttt gattgcattt ctgcttgttt ttctttttct
    19861 ctcctaatat tttctacata ctcttgatta ggctctggac ttaatacctc aggatagctt
    19921 agcatgaatc caacttcttg acctatctta agttcgtagt cagttataag cattctatta
    19981 ggtattttaa attgtccaag tctacctttt aggtctatag tcactccacc acctttgatg
    20041 tcaactataa taccttcata aaaagtctct gatgagatat attttaacct atccattata
    20101 attctacctc tctatactgt tttaaacttg gatctaaatt tatgttattg tctaaatctt
    20161 agttaatatc taaattttag ttttcgtctt tttcgtatat ttctcttctc ttcttactca
    20221 ttggtaaaga ttgctcattt tcttctaata ctattttttc tcctgtaact ttttctatag
    20281 cttctatgtt atttaattta acatttggat tccattttct ttcaggagct ttaacttcta
    20341 caccttctat agcattttta agcatagcca ttattctaac agcatcttct ggagctaaag
    20401 tattgttaga taatatttca ttctctatac cttgcttaga cttattgttg tctaccatgt
    20461 gagtcatgta tttattacca acaactagag caccttgaac agctgagaaa cttacaccaa
    20521 ctactggtat acctctcata cctatttgct ctatgtgaga tgcgaaatct atatggttgt
    20581 ttccaaaacc ttcagtagtt actacagctc catcaacttc catagcttca actaacatac
    20641 ctaatctctt agatacatag aatttctcag catttacttg tggagaacca acaaatacaa
    20701 cacctactaa atctatttct tcatcttcca tagctcttat tactaatggc tctctccagt
    20761 aatgtcttga catttctttt gatgcaggtc ctatacaagt taatgcatgg ataccaccat
    20821 ctaatacttc aagtggagat aatacaactg gcatatttcc taagtcaacg tttggttgag
    20881 ctcctaatgt accaactggc tcaactggta atattaggtt atcatgcatt gcaccttgtc
    20941 ccattatttc ttttataaca acaactttct ttttgccttc tcttctgtat tgctctatta
    21001 cttcttcatc aactactaaa gagttgtcaa ctttctttaa tgcttctctt atttcttgag
    21061 ttacatagtc aaatgcacgg tgagcagcta aaggtcctgg tctttccatg ttagttcctg
    21121 cttttattgt tacttgacct ttgatgaata tttcaccttt gtcagcagca cctggtctac
    21181 cccacattat agtagtattt aactcacctt ctgaagaacc aaattcacct atttgaactc
    21241 catcttcatc tgtaccagtt aataccatta cagctccatc tataactcta gttatacctg
    21301 aacctaattc gccttcttct ttagtagcga ttggttgtat atctaataca gcttcactgt
    21361 aagtttcata tttatcagga gttattattt ctaacttcat atctacaact aactcttgag
    21421 tttcattagc ttctttacat atttcttctg catttctgat gtataaagta gttccttcta
    21481 ttttagtttc atcagcaaat tcaactttat ctattttaaa gtgtttttta gttaagcttc
    21541 ttaaaagttt cttttcttca acttttggag ctacttctgc tgctgcactt gccactggag
    21601 cagcaacgtt tgctactgga gcagcaactc ctgcttgacc agctactgat aaaggtatct
    21661 ctaagcttat gttcttacct tcacctatgt gtattttaac tgtttcacct gcaactcctg
    21721 tgcttactgg aactacagct gcttctgata ctggagctgc ttcttctact gtttcttcta
    21781 ctacttcttc tttagcctct cctgctttgt atccttctac gttatctgta gtcattggag
    21841 aaagagcatc taaagtttct tttaacttag ctcctagtac ttgacctata gttaatccat
    21901 tttctggtat tgttaataat ccagaatcct ctaagtctgc aaatattgct ggatcttcta
    21961 agttttctgg cgctataatt gttcccgctt caaatctaca acaacaaact gctgggtcat
    22021 ttgcatgggc ttgagctgtt tctaaagtta ttgacatagt ataaaatacc tccttacatt
    22081 attttcttat ttattatttt aaatctcaat tcctcaacag gtaacccctt aactgagaaa
    22141 ttaagtaaat taaaacctac ataatttgtt atgattaata ttattttttt ctcacttaaa
    22201 gtcactttat attataagca attcctatgc caacttatta acaaaaagat tttttctata
    22261 atagattctt catctatcgc tatttttagc caaatattat ataaatattt attttaaata
    22321 tgttattttt tgtatttgta aacattttct caaataaaaa aagtgtcaat taatcaacac
    22381 tttttcattt tttttaacac atctatatat acgacactca tacaagcatt ggtctcacta
    22441 ggttttaaat taaagtttat cattttgtca aaatttagtc atgtgtcata ttttagacac
    22501 atttattttg tattggtcta tcttataata caatgttgtt ctgggtatat ttaatatctt
    22561 agctgcttta gctttatttc cattagacat tttaagtgcc ttggttatat tctttatttc
    22621 aattttttga gtggccttcg ttaaatctaa gggatactct tcttcttcag tactattatt
    22681 tgtagaatcc atcatataac ttggtacatc atccacatct attttagaat tttgtgacaa
    22741 tacaacaata ttttcaatcg tattttttaa ttctctgata ttgcctttcc atctataatt
    22801 ctgaaatata tctattacct ctttgctaat tgtaagtaca ggcttattat tttgtttgca
    22861 tatctcctcc aaaaaactat gaaccaataa tcctatgtct tcttttctct ctctaagtgg
    22921 aggaatcttt atttcaacaa catttaatct atagtataaa tcttctctaa atttttctgc
    22981 ctttaccatt ttactcaaat ctttattggt agctgatatt attctaggat ttatttttat
    23041 cgtagtatct ccaccaactc ttcttatttc tttttcctgt aacactctaa gcaactttgc
    23101 ctgcatacta agaggtaaat cggcaatttc atctaaaaat acagttccat cctttgcaag
    23161 ttcaaatatg cctattcgac cttttttact tgctcctgta aatgcacctg attcataccc
    23221 aaaaaactca ctttcaaata actcatttgg tatagcacta cagttaacag gtataaattg
    23281 accttttctc tcactataat catgaatagc tcttgcaaat acttctttac ctgtaccact
    23341 ttcaccccaa ataaatatac tactatttgt tctagccact tgttttgcaa tcgctttcga
    23401 tttttccagt ttataacttt tacctaaaat cttatcaaaa ctacctttag ataaattttt
    23461 gacttcgttt tttagatatt tcaatgtatc atttactttt tcaagttcaa gggctaatat
    23521 cttagcttct gtaacatcaa cttctgtaca tacaacaccg ataaattctt ctttataaaa
    23581 tataggattt gcatggacta atgcatacat attttctttt ttatccgtaa aatacaggtc
    23641 actcattcca actttactat ttagcactct ttctgaaata gtattttcca aaaactcatt
    23701 catcggtcga ccaactatgt cttcagactt tatatcatac ctttcttcca taaacttatt
    23761 ccacaagata acaacacctt cattatttat agcacaaatt ccctctttaa tttgacctat
    23821 aatatacttc aaagtcaatc cataatcttc taaatgcata tataaatagt ctctaatatg
    23881 ctcttgtttt agaactccaa taattttatt atctctaaga acaggaagaa tccctatatt
    23941 ttttaatatc attatatctc tacattcatc taaggttaaa ccttcattga catatattac
    24001 atccttcttc attataagtt ttactggttg gccttcatac tttttatgca tctcataaag
    24061 attgtgtata tccgtcatgg atataatgcc ttttaattga ccagatgaat ctattaccat
    24121 tagagttttt gtattacttt ttatcatttc ttttattgca tcttcaattc ttgtgtcttc
    24181 gtctatagtt gtaaattttg tatccattac ctcttctact ttttttactt ctggtatcga
    24241 aaacataaag tccccccttt ataatagtac atcatcaaaa cttatttata tatatttatt
    24301 ctagcatatt ataataatta tttattaaat aattattatt aaaatttatt aagtaactag
    24361 catttaaatt tatataatca atttaaaatt tataattaaa tttatacaaa tgaaatcaaa
    24421 tgaattatac ccttagtata ttattttttc aatactattt tcaccttcaa aacatttttc
    24481 tattcatcaa acaactaatg aatacctata tctaatcata agtttaagaa tataattaaa
    24541 atagatactt ttaaacaagc tatatttaca tattatattt acataaaaaa gtcccaaaat
    24601 gacttataaa tcaatttgag actttttatt ttaaacttaa taataatcta gattaatttc
    24661 tttttatttt aaattgataa gctaatccac aaatggcaat gatactaata aatacatatg
    24721 acattagctg agtttcatca cctgtttttg gtgattttac tattgtgtca tattttgata
    24781 gttctggcat tttttccact gtatcactta ctggtggtgc tggtggattt aacactgcat
    24841 catctgttgg tggcactggt ggatttagca ctgcatcatc tgttggtggt actggtggat
    24901 ttagcactac atcatccgtt ggtggcactg gtggatttat tactgtatca tcagttggtg
    24961 gtactggtgg atttattact gtatcatcta ttggtggtga aggtttaggc ttatgtccat
    25021 ttgtattatc tgttggtggc accggtggat ttattactgt atcatcagtt ggtggcactg
    25081 gtggatttac aacatcatct gttggtagac ttatgttaaa gttaacttct tggccattct
    25141 ttttctctac tgtataactt aataaatttt gcttactaga tggcaatgaa gatgagtggt
    25201 ctttttgtcc taaatcgatt actgccttat aatattgaat agcttttgga aaaatataat
    25261 cataagatat agcagaaata ttagctttgg catctggttt tccaacactc ttaagtttaa
    25321 atttctgttg tgttgaaatt tctacttcac ctgtacctat tatattacca tctccatcat
    25381 aagcagttac tccatctggt agtcttaact ttactacacc actataacct actaaggcta
    25441 cagcttctgt ttcataagta ccatcttcat ttttttcaaa gttaatctta gttaaaccat
    25501 tacttaaact aggttttcct gtatcattcg gctttaactc ttcacgacct gcttttaaca
    25561 atgcttctaa ataagctttt gtatttatcg caacttcttt cttcttttga tactcagctt
    25621 tttcttcttc attttttgca ttttctataa gaaaatcaca aaagtttata gagccttgat
    25681 actgcttaag tttttcttgt tcatctactt gtccaagaat aatccaaatt gcactttgag
    25741 tggctgagta agtcattatg tcattttctt ctccgcctaa attaaattgt tgcttatatc
    25801 caaatccatc aaattcagaa cctgcatata gtatagattt aactgcatct tcattctggt
    25861 tttcttgacc actaaattct tctgctggta aatatggact ctttccatta ctacttggtg
    25921 gtcttaaatg gttattgtag caaaatgcag tttgctctcc atcttcacct tctattacgt
    25981 atttgttatc ttttgagctt gctcctacaa gaccttttat tacatagtca aaagttaaag
    26041 tctttgtaat atcatcaaat cttaaatcat gataaggtct gtctgaacca tctatttctt
    26101 ttagtaaaca agtatttttt acttttgctt ttttatcata ccataagctg ataaatgagt
    26161 cttttgaagt aaagacttgg ttaatttctg attgttcatc tgactttaca ttttcttcat
    26221 cttcccaaaa gttatatact attttattta tttgagataa agtcatatca agaattttat
    26281 caatatcttg ctttagactc atttcttcta taactatttc ttcttctaat gatttttctc
    26341 cctctggagt ctgaccctct aatggttttt ctccttctgg agtctgacct tctaatggtt
    26401 tttctccttt tggagtctga ccctctaatg gtttttctcc ttctggagtc tgaccttcta
    26461 atggtttttc tccttctgga gtctgacctt ctaacggttt ttctccttct ggaatctgac
    26521 cttctaatgg tttttctcct tctggaatct gaccttctaa tggtttttct ccttctggag
    26581 tctgaccttc taatggtttc tctccattaa ttattgtatc attgcctatt acttcatcag
    26641 cataagatac tattggtgta aatgcagtaa ataccatact aaaagcaatc accacactga
    26701 ttatacttga ttttttaatt tttgttttca aaattctcct gatactaaaa acatatattg
    26761 tattcattta ataattaaat attatatgaa tcttattcaa caatatatct ttagtgtgtt
    26821 caattagggg cgttactcct aaccagttct cctatgaact tcttaatatt tctattaagc
    26881 acagactata tcattaccta accataaatt aggtactccc catttcccca cacttgtagg
    26941 tacgagattt ctctctagtc gttgaagttt cttcttttcg aagcttacct gctgattatc
    27001 cattttcaag gcacttagga tttaacctta tgctatctaa ctaatttttt ctactttcgt
    27061 aaccttaagt gcttattaaa tacacactct atcacactta tccttatttc atgattatgt
    27121 tgtggtttag ttagctttag gaacttccag caattaaagg agttttgggc aggtatctgc
    27181 cactctacac tcgttatgaa tgtagggagc atttcaaaaa tatatttata tctatcttct
    27241 actaattact tatttatgta ttttatatat ttttgacaat taaaagttac tcctatttat
    27301 atttatagca ggttgcacta ctaactccat atatctttta agtgcccaag tttaagatat
    27361 acttcacagt ttctataaat aggtttttta atttttcttt tttaactttt taagtgcact
    27421 tttttaacaa cttaataaaa tataaactag tatccaagat acttatctaa taaaagacaa
    27481 aacttagttt ttacactaaa aaagatacta tagagtatct tcaaatgtca caaaaacctt
    27541 ttactctatt ggatagttga cttaatataa tatacatatc aatacaaatc acatatttga
    27601 gtatcattat ttttgtccaa tattaattgt aatagctatt gttatcatta tacaatatct
    27661 agtatatttt gtctattttt tccatttatt ttaaaatata tcttaccctc aaagtacatt
    27721 aaaaaacatc cttagaagta acttttaagt tttacttcta aggatgttta gcttctattt
    27781 atctattatt ttatttttaa tcttacatca ccttttattc ttcttattaa tgggtgatta
    27841 actgttctca ttacatggtc agtaatatga tatacaccta attttaattt tggtgcagaa
    27901 cccattactg tgttactaca gtcagtacag tttcctatta atataacttc tgctccagct
    27961 ttcttaaatt ctatacattt ttgagcttgt cctggacagt gtcctggatt ttcgcatttt
    28021 aatgcacctt taatttcttg agcttgttta catttttgaa ctgatactat atcagtaact
    28081 cccattttag ttgctatatc tctcattctc tcttcattag gtccacatgc acagactaaa
    28141 agacccattt ttctcttttc attaacaaat ggcatagtaa ctattatacc accactagtt
    28201 ttagttattg gagcatctaa agtagtagct ttaccagtga aaggtcctcc taagattatt
    28261 tcaccatatt caccttttat tccaccagct ctttcaatta attctccaac agttgttcca
    28321 attggcacat cttcaaatac tatagactct tttccactat ttaattgacc aactacagtt
    28381 atatttttag atataactgg tcttttttgc tcaacagcct ctactattct agttaatgtt
    28441 tctacgttta ttactactgc attagcctct gaaggtaatt gagttggctc aagtaatttt
    28501 cctaaaacgt ctcttactat tgctctctct tctcccattg ggtacatatc aactagttct
    28561 gcaacttcta tgttgtctcc tggctttatt acttttttga aagcttctat tgcttttgga
    28621 tgtttgcttt ttattgctag catacctttt ccagcattag taacttccat agcatatttt
    28681 ataccttgat aaactatttc tggtctttct tctatttctt ttatattatg agctaataaa
    28741 ggttcacatt cagctgcatt tgctaatatt gttccaccat ttaaatctat gtttaatttt
    28801 acatgagtag ggaatcctgc tccacccatt cctactactc cagcttcttt aattaagtca
    28861 agtatacctt ctccttttaa tggttcaaaa gtatcatctt gtacttcatc agcttcaatt
    28921 actatcgctt ggtcatttat ttcacttatt tttccactaa cactggcata aatatttgcc
    28981 cctaatccag ttggttctgc gattagagtt ccttttttta ctacatctcc aacacttact
    29041 actggcttat ctggaccacc aacgtgttgt tttaataaaa tagaatatag tttgttcaac
    29101 tagtacgccc ccttaagtat attaattttt ttataagctg ttcaaataat gttaatgcta
    29161 tttttctatt ataaggtctt aacatatcgt ttatttctat agaacatatc gcttattaac
    29221 aaagcaattt ctatgccaac tttcaaataa acttataccc tttggaaata ctatcttaca
    29281 gcatttatta attcttctaa atttttaagc ccgtcaaaaa aaagtcaatt attagacact
    29341 tttttcaatc aatttaatct ataattattg atattgcata catgtataaa aagtgtcaaa
    29401 aaatagacac cgtcttttta tcgacacttt tctaacatat actatataat ttaccacaac
    29461 tttgcttaaa atatacattt attacttatt aattcttagt tttttgcact ccattggaat
    29521 caactacata attatcttta tacaacaaat cggatatttt gactagactg tatccatctt
    29581 tttttagtcg acttattata gtatccaaat agttatctac atcatttata ttcgcatgaa
    29641 ataaaactat tgaacccggt cgagattctt ttattacttt ttcaattacg tgatttggtc
    29701 caagctcctt ccaatctatc gaatctacat cccacttaat tactttataa cctaaatcac
    29761 tacaaatgtc catagctttg ttgtttacat ccccaaaagg aggtctaaat aaattagtct
    29821 tttttcctgt tatattatat attttttctg atgtagtttc tatctctttt gttatgtctt
    29881 cttttgatat ttccttcaaa ttggcatgag tatttgaatg atttccaatt tcatgtccct
    29941 tttcatctat agcttttaca atttcttcat tatcatctac ccaactacca actaaaaaaa
    30001 aggtagcttt tacattatgt ttatctaaaa tttttaaaat atcatccata ttatcagttc
    30061 cccatgctac atcaaaactc aaagctattt ttttctcatt tgtatcaact ttatatatag
    30121 gcatttttga attgtcagat ttagaacata tgtattttat tccataaaca aaaacaaata
    30181 ttaacactaa taataatgaa aaaaataaaa cactatatat caaagcttta gtccttttaa
    30241 cttctctttt catactattc ccccttttaa ataatttctt ctatatttaa aatttatttt
    30301 ttttcagctc aaaaaagtac tgtaatattt aatttataag acaataacta aaaaaataaa
    30361 acctatattg acctaattaa actctctaaa atactcgttt gtttaatttt tttatatttt
    30421 ttacattttt acctttattt ttctgtatat attattttta ttttggagaa attagtatta
    30481 acatcacata aataaaagtg ttgttagaca tcataaacta attatttgag gaggaaattt
    30541 actatgtcaa attcaaacag aacagtagtt ccagaagcaa aagctgcttt aaaccaaatg
    30601 aaattagaaa tagctaatga aataggttta tctaactatg aaaacataga caaaggtaac
    30661 ttaacagcta gagaaaatgg atatgttggt ggatatatga ctaagaaatt agttgaaatg
    30721 gctgaaagac aaatggctgg aaaataaata gtaaattttg acacctgggt atcaacaggg
    30781 ctgctaatta gtagccctgt ttctctatat ttatttataa aattgttttt atatgaatta
    30841 ttaaagatta ataatacaat acaaattgtt cttttaattt tgtacaaatt agtttaatat
    30901 attatatata gaaatagttt gacggaggat gatatttatg tttgaaaact cgtctgaaga
    30961 attagcctat cacaaacttt taattttata tatattagaa aaaattaaaa tggatttaac
    31021 taattctcaa ataactcaag tagtattaga gactgaaatg atgaattatt tttcattaca
    31081 acagctttta tctcagctta tggaatctaa gtttttgact acatataaag actctgacag
    31141 agaatattat gctcttactc aaagaggcgt agaaagtctg gaatacttct ttaatcgaat
    31201 acctactagt gttactgaaa aaatagacaa gtatatagat tctaacaaag aaaatttact
    31261 agcagatact caagtaaagt ctagttttgt aaaacaaagt gataatgaat ttattgttaa
    31321 ccttagagtc atagaaaatc agtctaactt aatagattta aatctaaatg tttcatcaga
    31381 gaaacaagca aaacttattt gcaataactg gaaaaataat gcatcttata tgtatgccga
    31441 agttatcgac ttacttataa gagacataca ttaaagccta caaacaaatt atagttgtag
    31501 gctcttttcc taccttgatt tcttttatta atttgttatt tgcagtttca taaactttta
    31561 ctgaattttc ttgtgtatct gatacaaata gtaacttttc atctttagat atttctaagc
    31621 cctgaggaat cccatttatt tcaatatttc caatgtatct actcgttgta taatctaaaa
    31681 ttcttacatt attatatccc aaatcagcaa cataaacttt tttgccacta taattaaatc
    31741 ttatttctac tggtaataaa aattcatata ataccttttt tatatccatg cttacttcat
    31801 ctattattat aagctttcca tcaagtgttg atatatacat ttctttcttt tgtttatcaa
    31861 gttctatatg ccaagctcta aagtccatat ttattttctt ttgtatattt ttattaattg
    31921 tatctataca tactatcgag tttatacacg gtacatataa ctttttactt tctctatcaa
    31981 agtcaaatcc atgtggcata ttatcaactc caataattcc tattggattt aatgtgtttt
    32041 tatctagtac atatatacta ttagaatctt catttgctac aaataattca ttatcgctaa
    32101 gtacaacttg actaagactt ccacctatag aaacagtatc aattatttta tcattgctta
    32161 aatctaagac atagagtatc ccattacttg aactaggtat gtatattaaa tttttttctt
    32221 tttctatgca aaaatggtgt ggatatatat tatcttctaa tactatttct ttttctagtt
    32281 caagtgtaga ataatcaact atgctaatgc tctttgataa atagttagaa atatagattt
    32341 tcatataacc cctcctctct acaatacata tactacaata atatattatt tcattagctt
    32401 gtattatgct ttatagttgc attattactc atatatttgg tattattatt aatgttaaaa
    32461 aaagaaataa aatttaatgg aggtcttttt aatgaaagta acatttaatc caagtggtgt
    32521 ctgttgtaga gaaatgattt ttgaagttga tgaaaataat gtgattgttg atgctgaatt
    32581 tataggggga tgtaatggta atttatcagg acttagaagc ttaataattg gtcaaaatgc
    32641 tcttgaagtt gctgataaat taaacggtat tgattgtggt ggaaaaggta cttcttgccc
    32701 agaccaatta tctaaagcta tacgtcaatc tatataattg cttctataaa tatcaataaa
    32761 attattaaaa acttacttta tataaaaaga gttgtccatt ttttagacaa ctctctttta
    32821 tacttttatt tatatttgta tatttatttt ataaaattat aatttcttta ctaaagttct
    32881 cactgtacca atcatatata atgaacctgc acttattata atatcatctt cacttgacgt
    32941 ttctaatgta taatttactg catcctctat ctcatgcttt gatgttacat catctacata
    33001 ttttaatact ttttccctta atatatctga atttatggcc cttggattga ttggtgttgt
    33061 tgttattacc ttattaaaat gtggtaacaa tacctctaaa acactgtcaa tatctttatc
    33121 ttctaacata cctattaata aggttaattt tctatcttta aaattcttat caagtgcttt
    33181 tgccaaagat tttgctccat cttcattatg tgcaccatct attataaaaa ttggattttc
    33241 ttttatcttt tctattcttc ctggccattt agtctttata agtccttttc ttatagattc
    33301 ttcatttata tttgctaatt tatttatgtc ttttagatat tttattacac taattgccaa
    33361 tatagaattg tttatttgat gttcccctat taattttatt tcaaggtctc tataattttc
    33421 tttcattaca gtacaatcgt atatttgaga atttatatct gactttttta tgtttacatc
    33481 atcaaaatta acttctatat atttagcttt tttctcctta caaactgact ttataacatc
    33541 ctttgcttca tcagtctggt catatactag tactactcca ttttccttta ttattccagc
    33601 cttttcatac gctatttttt ctattgtatc tccaagtatc cctgtatggt ccaaacttat
    33661 agatacgatt acacttacaa gtgattttgt tattatattt gtggcatcat atctgccacc
    33721 taaaccaact tctaatgcca caaagtctac cttttgctct gaatagtaat aaaatgccat
    33781 tgcagtaacc acttcaaact ctgttggata tgcatagcct tctcttacca taatctctat
    33841 tttttctttt attaattcaa ttattctagc aacatcatcc tgtggtatat tctgtccatt
    33901 tacccttata cgctcagtaa aagtttctaa atatggagat gtatataaac cgactttata
    33961 tccacactct cttagtatat tagaaacaaa agaacacaca gaaccttttc cattagttcc
    34021 agctacatgt attatattca aagtttcttg tggatttcct aataattcta ataactttcc
    34081 tatattttct agacctaatc ttataccgaa tttattagtt tgtgatatat actctaatgc
    34141 ttcttcatat ttcattatct aattccccct gtattggcat tttctcttat gctatctaat
    34201 tatagtatat tttttgcttt taatctactg acaaaggata tatataattt aataccttct
    34261 acattaaatt tagctacaaa tctttattta taagttttta tatctttcca ctaattcagt
    34321 cagtgtatat attgaaacta agtcatattt tgagatatcc atataattaa actttacatc
    34381 aaatctatac tctagctttt tcaataaatt aaaacaatta tcataaacca aacctatctc
    34441 cttattaaaa aggctacact taaatagttt ttcattatta tcttcaatat ttaaacaatc
    34501 tctaacaacg tcagcaactt ttataagaag atcattttct ttgtcataca aatccaattt
    34561 aaagtttaca attctactac actcatcaaa atcacaacta ttccaatgat ttctaattgc
    34621 taatgattca agcttatcat ttaaatcaga tattgttatg cctggttttt ctgataatat
    34681 cttagctatt ttaccagtaa gctttgctga caaataacta ttacattttg gtggcaatct
    34741 ataggaatta tttatgtcac aacttatata tgaattgcta tccataacac attgaacatc
    34801 atatttttta ttgtaccaaa atgaatcatc tacatctaaa gtaagtcccc ttacacctat
    34861 aacattgtta aacatagctg gataacttaa gtcaaaatca ttagcgagtg aacatactat
    34921 tatctttcct tttctaaaca attcatagca aacttcaaac aaacccttaa cactttctac
    34981 tcctatgact gataaactta aattaattaa tcttatgtta gtgtctaaaa ggtatcttaa
    35041 tgcttcttca agaactttta tatttgtaat accatctttt ctcaaaattt ttattacaaa
    35101 taactctaca tcttcatact ctttctttat tattgatgca caagcagttc catgaccatc
    35161 ttcatcatca aatttatctg atataaagat ataatcatgc atacattcaa aagcaattcc
    35221 tccaactaaa ttatctttta agtaatcatg ttctttgtct attcctgtat ctaatactgc
    35281 aaccttaact ttattattca tatcacatat attctccatt acaaaagttt ttaatatatc
    35341 tatgttaata gttcatctta aactttgatt taagttgcac aatttatctt ttaaatgcta
    35401 tatttcttgc taaaaacata
//
LOCUS       DAFNNB010000021        35100 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0021, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000021 DAFNNB010000000
VERSION     DAFNNB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 35100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0021"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..775
                     /locus_tag="L3M60_000936"
     CDS             <1..775
                     /locus_tag="L3M60_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6225630.1"
                     /translation="RIKLEKPKTSKSFKESIEKLHKYEPDLIIAVGARFAEPIKEAAT
                     KYSKQQFAIVDYTYKVQPANVISVSFQDNATGYLAGLIAGKMTDTGKVGFIGGVEGSS
                     RDKYESGFRKGLKTSNKDAELLVKYSNIFENPESARTIAKEMRDSDRDVVFSATEDDS
                     KRAIQVARENGGKVIAINKDQYDMAPNNVISSIIKDVEQPVYELIKNVVKDGFKGNKV
                     MEFKIKDGELGLTSKSSRNIPPDVLEYIKKEYNKVKDTY"
     gene            930..1691
                     /locus_tag="L3M60_000937"
     CDS             930..1691
                     /locus_tag="L3M60_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225631.1"
                     /translation="MEFTREPFILDDDLCLALVDKRISYLMENELKLRNIEIIKTVEC
                     KELYESIKYHPDICICNLGKGDIIVAPNVYNQYKELLSRYKFNIIKGEANLLNRYPYD
                     IPYNIAIVGDYAIHNFKYTDKAILDYIENNKLKKINIEQGYSKCSICIVDRTSIITSD
                     KGIWKSMKNTEIECLLIEKGHINLFEMNYGFIGGCTGLISKDKLAFCGDVKKHPDYER
                     IKSFVESKNKEIVTLSCENLLDIGSIVPLMTRKER"
     gene            1694..2512
                     /locus_tag="L3M60_000938"
     CDS             1694..2512
                     /locus_tag="L3M60_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sporulation protein YtxC"
                     /protein_id="HBN6225632.1"
                     /translation="MVEKDNISSMKFLRNNNVNDRLIYTNDIVEVVIDSAKQEVLNKK
                     ILNGTKAEQRALNIDEISTEITSMIISIMKEKLLKEYILKCYGRTYPEDKNNIYIYSL
                     NIFAKKEIFMRETVFTRVRNYIEENDFINVEGFIRFRMREFMKYISAIGDIAVEEYLI
                     KKDQDEFIRVLKYFIDTQEEKIDLLKVHIMEDNTFVLYDKNGNKIDSIDDEEIINMVI
                     RENLNYEDFLISTLLTLCPKKIEILDLLNNNCSKEIIDTVEAIFENKVSIIMEN"
     gene            2691..3293
                     /locus_tag="L3M60_000939"
     CDS             2691..3293
                     /locus_tag="L3M60_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HBN6225633.1"
                     /translation="MDNLIRILILAVIGGFIGYVTNVVAIRLIFRPIEPIKIPILNIE
                     IVGLIPKRRVEIAANVGEIIQEEFLSMDEILANIITDEDKEEVVRYIKARVKIIIHEK
                     VSFIPGGIKNMIQDYLGEIIESEVKKSIDELSKNIINKANERIDIQKMVEDKINELDL
                     YELEEIIIRIAKKELKHIEFLGLVLGFLIGIVQGLVTMFI"
     misc_binding    3783..4022
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4086..6005
                     /gene="thrS"
                     /locus_tag="L3M60_000940"
     CDS             4086..6005
                     /gene="thrS"
                     /locus_tag="L3M60_000940"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBN6225634.1"
                     /translation="MIKVALKDGSIKEFENAISVMDVAKSISEGLARNVVAASVNGEV
                     VGLDHIIDTDCDLNLFKFEDKEGKEVFRHTSAHILAQAIKRLYPEAKLAIGPSIENGF
                     YYDIDLDHRLVPEDLEKIEAEMKKIAKEDLKIERFELPRNEALELMKEQGEDYKVELI
                     SDLPESEIISFYKQGDFTDLCRGPHLPSTKKVKAVKLQSVAGAYWRGDENNKMLQRIY
                     GTSFEKNKDLEEYLHLLEEAKKRDHRKLGKELGLFMIPEEGPGFPMFLPKGMELKNEL
                     LKFWREIHRKAGYIEIESPIILNRKLWETSGHWYHYKENMYTVKIDDEDYAIKPMNCP
                     GGLIYYNSQLHSYRDFPMRVAELGRVHRHELSGALQGLMRVRAFTQDDSHIFMLPEQI
                     KDEIKGVANLIDGIYKTFGFEYNLELSTRPENSMGSNEEWEAAENGLREALEELGLPY
                     TINEGDGAFYGPKIDFHLKDCLGRTWQCGTIQLDMQLPRQFDNTYIGQDGEKHRPVMI
                     HRVAFGSIERFIGILIEHYAGKFPVWLSPTQVKILPISDKFMDYANEVKKELFDKGIR
                     VELDDRAEKIGFKIREAQLEKVPYMLIVGEKEVADNNVSVRSRDKGEIGSIKLDEFIA
                     SISKEIESRESIIQD"
     gene            6730..7821
                     /locus_tag="L3M60_000941"
     CDS             6730..7821
                     /locus_tag="L3M60_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBN6225635.1"
                     /translation="MIEKVGEKSKIIKTSKEYKRTIIEEKDREWTQKLNDILSRNNFS
                     NDELDKIKELIPKEILTSENVGEVVTEDGYARPEYDEVFKSLFTLNNEYGLLVNFIND
                     ILKDAPYANRNIKPFTQIKRIIRLETDPTINYIGEKRPRLDILAEDEENNHINIEMQR
                     AFENDYLERAEYYLSRVHGRKLEEGKEYKEIGKTIGIHILNHVKYNHIEDYVNCLRLT
                     MDGHPEIYSSKTALYFIELPKIHKSDYIVNRIMLWGKLIINPSSLDVRVIAKNDSIIK
                     KALDRLKELGSNEEYLLNLKRGAYIMNKSRNFKEEIERETAIRVAKEKDYKIIIMLKK
                     NGFKLDDILNKFNAFDLSEDEIKEVYENN"
     gene            complement(8169..8366)
                     /locus_tag="L3M60_000942"
     CDS             complement(8169..8366)
                     /locus_tag="L3M60_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225636.1"
                     /translation="MTIASDVIRCDTKTSILKHLLKKDSYSYEINKSILEKTKGLYEL
                     KEAILYTAFRRLEQSGAITSY"
     gene            8726..10066
                     /locus_tag="L3M60_000943"
     CDS             8726..10066
                     /locus_tag="L3M60_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBN6225637.1"
                     /translation="MIKSSLFWNIFIAISFMIEWFLCKKMLDYTSEKKISNKKINFCI
                     VGIIVFANIIYFFDAPLNIRVIISMIVTIAFYKISYDVRFIKSVLISLIYWMLLIAID
                     ALSISLTVWLNSINDMSNMGTGDIYRIESIILGKILLISALFLYRALKIKFNIEINKR
                     TLFYIVIPIVANIASFFVIFKYIFNLKFGNLIHNFQFVVISVVLLVSSITLILIAKKL
                     QQYNTLVVENNMIKNTLKCENEYYEDIEKKYLKTRLLSHDMKNHILCINAMIKKGIDV
                     TSYLNNLQVEIQSNDLLFNTGNFILDAILSEKKEVCDRNDIELKIGINFSCCEAVELV
                     DICHIFSNILDNAIEACNKIDSGNRVICVKGDIMQNFYLIKVENTKTNKIITKNGDIL
                     TDKKGSLHGLGIKSVKNSVNKYGGNVVIEYDDKKFVVKIAIPLRNVKKVVNNII"
     gene            complement(10408..10977)
                     /locus_tag="L3M60_000944"
     CDS             complement(10408..10977)
                     /locus_tag="L3M60_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBN6225638.1"
                     /translation="MSYMPTLEQAWEILRKYNKEEFHIRHAQIVSGVMRYYAKEYDPE
                     RVEFWEIVGLLHDLDFEMYPDEHCVKQRELMTELDLDKSIIDSTISHGYGLTGSDVKP
                     EQFMEKVLFAVDELTGLIGAAAIMRPSKSVSDLELKSVKKKFKDKSFAAGCSRDVIRS
                     GAEMLGWELDKLIQTTIEAMRSLIPEMAI"
     gene            complement(11138..12388)
                     /locus_tag="L3M60_000945"
     CDS             complement(11138..12388)
                     /locus_tag="L3M60_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225639.1"
                     /translation="MVKSLNTFNKNFNLMILGQIISLFGASILKFALSLYILDITGKA
                     EIFATILAVSSIPIIIFSPIGGAIADIFNRRNLMVIFDFSSSITVLVLALFLFNNNGS
                     ILLVGIIMTILSVISTMYQPTVQSSTPLLVDNEHLMNANGIVAGVASLTNIAGPVLGG
                     VLYGVIGINAIIVISCVSFFLSAIMEIFIQIPFIKQEQTSNIIKTIFIDINDGIVHIC
                     KENRFILKILFLAIAINLFLSSMLIVGIPYIVKITMGASNFLYGLAEGAISFSSLVAA
                     VSIGIFSKYLKITNLYISFTICALMFIPMAFSVYPFILNTGFPVPFLIFIVSSMLIFF
                     TTTLISIYAITIIQLKTPNELLGKVMSILFAGTTIAMPLGQVMYGKLFDLLSDRVYII
                     ILGVGFITLIIAFISKITLRNEKL"
     gene            complement(12397..12630)
                     /locus_tag="L3M60_000946"
     CDS             complement(12397..12630)
                     /locus_tag="L3M60_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225640.1"
                     /translation="MIELIQRLNDVIYKINDKLNIHTKLVDPSSELMPLYSMLSIIVS
                     IFLIILGIKVRKKVLIVIAIIAIIVNVLIILDR"
     gene            complement(12681..13259)
                     /locus_tag="L3M60_000947"
     CDS             complement(12681..13259)
                     /locus_tag="L3M60_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6225641.1"
                     /translation="MAKSFTEIEKDNIRKRLISECEINWSKYGYKKTNIDELCSKSGI
                     AKGTFYTFFDSKEKLFFEVLNGIQNRLISSVDEILGNMPQKEGFAKALKMLYRLYDKN
                     PFLYSPNNTDYITLLNKIPKEMLSQLEYNNVQSFYSLIERHNLKLKIEKEKAFGVCNA
                     LLSSLLLKEDIGYDYIEVFDFMVDNLVEHILE"
     gene            13560..15020
                     /locus_tag="L3M60_000948"
     CDS             13560..15020
                     /locus_tag="L3M60_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HBN6225642.1"
                     /translation="MFNELRTKENVYKNLINGKWVESNSRKPIEIYSPIDNSLVGKVQ
                     SMTKHEVDEAIKNTKESIKVWADMPVYKRANIFHKAADLLLENIDEIANILVLEIAKD
                     MKSARAEVERTADFLRYTADVGKNMEGEAISGDNFPGGTRNKMSYVSRVPLGTVLAIS
                     PFNYPVNLSMSKIAPALIGGNAVVLKPATQGAISALHVVEIMRKAGIPDGVLNTITGR
                     GSEIGDYVVTHKGINFINFTGSTEVGQHISKISGMVPLLLELGGKDAAIVLEDADLDF
                     AAKNIVSGAYSYSGQRCTAVKRILVQESVADKLVEKIKPLVENLTIGNPKDEVVITPL
                     IDNKATDFVQGLVDDALHKGAKLIVGNIRKNNLFYPTLLDNVNTDMKIAWEEPFGPVL
                     PIIRVKDISQAIEIANQSEYGLQSSVFTSDIDRAFYIADKLEVGTVQINNKTERGPDH
                     FPFLGVKASGMGTQGVKYSIEAMTRPKAVVVNVRGL"
     gene            15389..15994
                     /locus_tag="L3M60_000949"
     CDS             15389..15994
                     /locus_tag="L3M60_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002591427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6225643.1"
                     /translation="MPKGIVLTPEQQAERREKIVAVALSLMAENGFQKTSMREIAVLA
                     NMGKSSLYDFFKTKDEIVVYAVEKEIEKTIQQVHRIIDDELSPQQCLRKIMLNHLRFP
                     KQYRTVLMWLNTESDYLEEEYRKRLKDARYAYQDIIKSVIDNGVTTGIFRKTDTALVT
                     RLLINSMLSISYTSRLSDSSEKMLDETMNIFLHGIMNDGGE"
     gene            15997..18564
                     /locus_tag="L3M60_000950"
     CDS             15997..18564
                     /locus_tag="L3M60_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002602373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HBN6225644.1"
                     /translation="MIYYVLPLEHKQATIEIVGGKGMSLSKLLTAGIPVPDGFHVTTA
                     SYQIFVETNHIQSRINKLLDGIDSNNTSQLEDVSKKIGVLFHNGEMPQEVSDAIKTAY
                     DGLGNISVAVRSSATAEDLPDASFAGQQETYLNIQGEDEVIDAVKRCWASLWTARAIA
                     YRVKNNIKHEIVALAVVVQKLAFSDSSGIMFTLNPINGRRSEMIINAAWGLGEAVVSS
                     LVTPDTIVVDKDSERIISYEVANKEIMTVRTSEGTEETMVPERLRKKYTLTRNQVMQL
                     IQLGKKIEKYYQMPMDVEWALEKDKLYIVQARPITVLPPEWVLPEQDVVYTKGSLAEH
                     LPNPVTPLFATLGLEIVNRASELLWIDMFGKSAKKLLPKNGAYTVINGYVYLSAKSKP
                     FLIAIKSLSPRSLRRTLMNSVARWETARKGYEDAIKQWEEKPLHTMNAHQIMEGIQTI
                     FYAACIYFTRIQLTLPAASISETLFTKLFQGAVRRAGITDTSVFLLGFDTIALQSEKE
                     LWGLSEWVKQNNSINLYLQNSPAIKIAEDFMSSVVPVGVSQEVWIEWKNRINKYFKEF
                     GRTAYEFDFAYSTPQETLTPTFESVKTFVEGKGESPFLRQTTFEKRRKHAEEEILQHI
                     GGLRKKLFFKLLHWAQETSPMRENAIYLMGMGHPLIRCMFQEISERFIRGGAISHLDD
                     IYWLTKSELEALIEQLDKNKSLSDMNGLIPVRKAELKKYMGYVSPSKLPEKNKKTISQ
                     ALQKQKDGKIVLTGIGTSTGVVTAPACVLNSPADFESFQPGSVLVAVTTTPAWTPLFS
                     SASAIVTDIGGPLSHSSIVAREYGIPAVMATHIATRTIKSGQMITVDGLAGTVTFDE"
     gene            complement(18824..20908)
                     /locus_tag="L3M60_000951"
     CDS             complement(18824..20908)
                     /locus_tag="L3M60_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6225645.1"
                     /translation="MSKYIDFANIEYSLLMNALDVSVSKHLLDEHFTVVWANDRYYEM
                     FGYTKEEYETLFHNQCDLFYKDNPEDWNELVEYVEKTFSTGAKKYDYVCRMKHRNGKK
                     LWIKLFGFLTGEVVDGYPISYSVMIDITEQMMIKIEQTVTYNNFPGLIAKFKVTDFGF
                     QLLDANKKYFDMFKDNVNFLLDDINLESYLSAVYKYQSDFKAGKYVCFSVSPQNINGD
                     IVHMQVVAECIDWEDGKPIYLLIYSDVTTLVKQNEIIEKNNKELEKLAFIDSVTGGMN
                     HTKFNIIAKEVISQSPSGTYTLIWLNMQKFKLINDLAGNIAGDKLLKYIYNCISKHLH
                     EGELVTRIFADKFILLLKDKPEDITSRLNSMVTHINRFNDNADYKYILYFTAGAYVIY
                     DTSLDITNIQDSANVASKSIKNVENHGLCICNFYSKKDKEKLILEQSIENRMYEALEK
                     REFQVYLQPKLELSTQTICGAEALVRWIHPVEGIIPPNEFIPLFETNGFIIQLDLYVF
                     EEVCKTLKKWIDDGLNPINISVNMSRYHFSSNNFIKTYDKIAKKYDIPSHLIEIEITE
                     TIVFEDVKVFSEIINEIHKNGFKCSIDDFGSGYSSINMLKDIYVDTLKLDRAFFQSEH
                     LDSQRERDIIVSIIELAKKLKMTTVAEGIETSFQKDFLQEINCDMLQGYVFSRPLPIK
                     DFEKLMFQNSSN"
     gene            complement(20956..21837)
                     /locus_tag="L3M60_000952"
     CDS             complement(20956..21837)
                     /locus_tag="L3M60_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6225646.1"
                     /translation="MPAYLFDSNYITNIYRVYSISSSFIVTGLYFCYKNHKNNQINFD
                     TVLEDKKNLNFLLSLMFVMYLYLCLSLSIYYTVGNVLILKLYGIKSAIIVLVGNYIAE
                     KYSFRITQIKSYDIKNQLEYLESIKTEHEINKLNDIVYTDLLTGFYNRPFVNQKLSEW
                     MSNHYKFTLCFTDLNRLKLVNDNFGHQFGDKYISTTAKIIKKLINVENSLLFRYGGDE
                     FLFLLQDTSCIEAEKIMIDINYELSKLSNTDEYPYALSISYGLVEWDGISDIETLIAE
                     ADSLMYENKLKNKSTLN"
     gene            complement(22187..23368)
                     /locus_tag="L3M60_000953"
     CDS             complement(22187..23368)
                     /locus_tag="L3M60_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6225647.1"
                     /translation="MKYHIFLIITIILIVELCNIYIYKIASFGNKSIAIHKNYKYIFL
                     IKPLTFLTIIFTKLPLPLFYILFYIVSHKLLLISIKNSDNKHFIPNIAVINFCILHLI
                     TISIMSIVENTSPDIIFVNMSSRIISILIASILDFILWYSMDKIKEHIYVITQNQIEF
                     SLFLKFIYFTLGYILFDSIQIAFSPPSALTSYFILGSNILLSLQMIIFINHIYSIEKN
                     IYLEKEHNRLKMIKQEQLLKVNLLKKKANIDELTSAYNRKFAFEKIQHYIDNSLLFSL
                     IYIDLDKLKTINDKYGHLAGDNYIINFSSIIISNLKADDIFARIGGDEFIVIMPKSNE
                     NTTKEFIEKIRKSMYNKISFSYGIVEVPSENKMTVEEIINIADIRMYTNKQNNHKRNS
                     N"
     gene            24495..24770
                     /locus_tag="L3M60_000954"
     CDS             24495..24770
                     /locus_tag="L3M60_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225648.1"
                     /translation="MSLIVGGWSKFRAVEEEDLKVFNEAVGMLKGVDYEPLIVATQIV
                     AGTNFKFICNATSVTNPPHDYLAEIIIFNPLPCDKQKATITSINIVK"
     gene            24790..25077
                     /locus_tag="L3M60_000955"
     CDS             24790..25077
                     /locus_tag="L3M60_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225649.1"
                     /translation="MRLNITKQNTIEVEGSKKEDVYFIIVDNVFFERKGNVFYSITNN
                     NLNNYDIKILPTDNIFLELKITSTKDLEPVFIVAEDRQYNHCSVMFKPVMV"
     gene            25497..28013
                     /locus_tag="L3M60_000956"
     CDS             25497..28013
                     /locus_tag="L3M60_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6225650.1"
                     /translation="MPEDNKTFLYELKELLSNGEKQIAKAIGQDLILILGGTGCGKST
                     AINYLAGCEMHKVEDEDTGVSYITCEDPVADIGSGAVSKTLYPEVIDMKKGFEGMTAK
                     FCDTAGFGDNRGAIHDICAAVSLGEVFKDSASICAVIVLIPEADILDSRLTKVLDLFQ
                     QLNMCLHKENFKNSITFFISKSFLGRTEKQIYNVIKRKYEQLCEASDSNMSDSNSNIK
                     EKAWLFEEMLKDDGKNIHICNPLASDDREGLLRQVLELEEVKDKISAFQYPISADALL
                     VLDTLIKEIQDNIVSSLEDYVKAYTEDFISAVGSITMIEEIAKYDDVVEVFKNWLEST
                     TSPTIGRFINELARQSSLFLQDSCMKLQKNIKENLDIIDALSEYTEHGTSEILLSSVH
                     MEFIIEKTSKKIMEQNFIIIRKNLEKVLVSYEVQKQVFTLPNRENLMEVANKKDYKYS
                     VNDFNSILTNVTVTQKEELEDSITRLNAYGEEGNEIIREFISKYILNPFIVKTININD
                     KQGIEIRALIPNLVISSVLEYFKNRKVDDNVSTFMFFAKNTIYMDDNLGMDFASEKNI
                     IIACNNLDVGSNVKINVSGKSGELVDNSMYGKNGEDGRDSGNIYLYIKEEIRNYRLTL
                     IANGGNGSKGIDGKNGETGASGSNGADAGYTSSFHGKMFKGYFAQGTSGGKGEKGGAG
                     GNAGRGGNAGKKGNIYFISDSEEQKNRIINRCDITVRDGEVGPNGRPGSGGYGGNGGY
                     HGYNTLSFSPSGIDKTVYYTGYFTSWDYKLDSIIKYHIEFSGGSWNEGKTGPGREYAE
                     YGDRGADGGFNAPNTKISTAVSKIEDNFIKEKYQCYCSEI"
     gene            28028..33238
                     /locus_tag="L3M60_000957"
     CDS             28028..33238
                     /locus_tag="L3M60_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HBN6225651.1"
                     /translation="MANKLYSGIVNLLEDGRDELRKYNLEKKSILLFLGASGVGKSTC
                     INYLKGCVMEEKTDAETGQVYITAKDSAVEIGNGVYSKTLCPEVVDIANRDFSLCDCP
                     GFFDNRGAEYMIAGAMLVRETISTSSKVKGMVVILDYQSLLDSRYTLLIETGKNLKDM
                     LGDYAKYKENFFFLINKIPKALISNVTEEKIRNILNKTKEDLDGQEIDYDGCKELIYL
                     LDVMLSDSVSIELCNPLDKNPEKLLDKMKNLPETFEKTNLGLMLTASCKIEIQKAVDV
                     TYKELEKLLVPYIESVKKGMEDSISTLSSINSLKDKKIQLDRMYTDLCGRANSMKELM
                     EFVQELSYGDGMQKVNILEETRLLLELNSYCSETHIVGLSELLKSLLKNTLEDTLKDI
                     KYWIAEEEYKEEEAKKLEDAEYRIEKIQSCTYIKSYEKENVLSIYIPIPKVNLSDLIK
                     IISGYKLDILSSIYMYGDCELKIDCSMEKDVFSGKNIVLLTEKVFIPEGSYSINVSGK
                     NAEKLAGEDYIYNGMNGRNGNNAGTSGDIILSFKEIEKIGELKLYVDGGRGGNGQDGK
                     IGETGLTGKAGENAKIGSKKESKEFAKDKDTKYYLVKAEKGKQGGTGGIGGNGGNAGL
                     GSKAGQVYLLGKATLLKGAISKKDGNHGLAGKGGSGGAGGAGGLGGIRGIYEETIRYI
                     GISAPIDDSDRNYYYFDRYVFNHASDEKEVADDGVKLNALYEKHKDWTISKREDSGSV
                     GAEGKDGLSGDTLKEKELLKQEHSLSIDTFFYEFFERFGFSGSKGYRNLRLLCAVGIL
                     EADILELSHIYEIFHAVEKIEMQLNREILQKYYGFEKKNKAVLSLDDMNIKKEFYKEA
                     KEIYEYLINKLMQSDRIESFIGEKQNFSEWSMILFALEQKTAFYVQMIEESEIGGNRV
                     TDIRSLTDYLDKILKELEEGKKDELKEGYFNTYKESILTQIESGNQQIAVLKNELENK
                     MLSVNAKMNLLIQEIIKIKEDEKEDIEKLKEKQAKLEEQMKTQLILSGIQSALSLLTA
                     FSGVGQACLGAFDSAKSLKENLSAKEEEYQWTNRMVDFSDFKLDSDGMRFVLGEQLSE
                     DRKNALDKMTYNLAGVDEGKRDKFDQIKEIIVKSIDDDNYSDKDDDIQRAKDGLSESE
                     YEEIYTKIQKKEIGLLEANYNNKQQKDKLAALEKSKQTIERQQKTSKIKNALSIGSSA
                     CSLINTAISGVNNCLKVKSDFESKIEVIKDAIKGKEDNIKKLDELNTQMVEFKDKTLK
                     ESVIPFVEKFDSTVKNQDIFENEISKLKMKEVLTELITKFGSLKMENSEEISIIFKKV
                     QDMMETQIIITDKIDKKKDNLAMGELIYQMSGSGSQMNEYQKKLIHQIELNQVRYLCQ
                     VEAAAFQLWTFPFGSNIVNILNRCHVGITEDENEVVNQAKEQNRQIKEFLDQDAYQWQ
                     KTDNHILKVALAGSLDGKSRVGFENTYSIYDCNEEEVKKLLTGDEISFEVPLNQNFDA
                     IKYVTMYANIPNLRRNILDDSLKVKVHIHLYDSGYFSLYDDKKDTYYQYVFPQNYIDI
                     THGLDVKYNDSDSINSIWSSVEDKGIEKFKKAGLNSGRSPYSMAKISLKAHFNKKLSY
                     DLLTNEQKKKLESYFDKSSKYAWSESIDILKSLGYIDLKGILSKSWYEIKKDIDSDGE
                     IMKHFSILQVNLLERIIYEQTSILKENIPNLTIELVGCGIYVESEFNYLKQNDLKCYE
                     ERLVENTIID"
     gene            complement(33490..34001)
                     /locus_tag="L3M60_000958"
                     /pseudo
     CDS             complement(33490..34001)
                     /locus_tag="L3M60_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888383.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            34136..34324
                     /locus_tag="L3M60_000959"
     CDS             34136..34324
                     /locus_tag="L3M60_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225652.1"
                     /translation="MNKNDNLVIICMFIGMILGVAIGYVMGIYKGSVGTTMCYGLVFG
                     MLIGICIGAVIKNSNKKE"
     gene            complement(34494..34934)
                     /locus_tag="L3M60_000960"
     CDS             complement(34494..34934)
                     /locus_tag="L3M60_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBN6225653.1"
                     /translation="MYERMLDKSNKPCLSQIIEYIGKNGYARLCKLETYLETQYNLSR
                     ELRFPFGNNYGWGYKYSHKSFHLFYAFFEKGAFTVMIQIGDKQVPAVEKMLISLTPKA
                     KEHWVTRYPCGNNGGWIRCRILEDADLLDIIQYIRAKKAPPKQN"
BASE COUNT        13413 a         3993 c         5931 g        11763 t
ORIGIN      
        1 taggataaaa cttgaaaagc caaagacttc taaaagtttt aaagagagta tagaaaaact
       61 acataagtat gagccagatt tgataatagc agttggagct agatttgcag agccaattaa
      121 agaagctgct actaaatatt ccaaacagca atttgcaata gttgactata cttataaagt
      181 tcagccagct aatgtaattt ctgtatcatt tcaagataat gctactggat atttagcagg
      241 acttattgca ggtaaaatga ctgatactgg taaagtaggt tttataggag gggttgaggg
      301 ttctagtaga gataagtatg aatctggatt tagaaagggg cttaaaactt caaataaaga
      361 tgctgaatta ctagttaagt attcaaatat atttgaaaat cctgaatcag ctagaactat
      421 tgcaaaagaa atgagagatt ctgatagaga tgttgtattt tcagctacag aagatgatag
      481 caaaagagct attcaggtgg caagagaaaa tggtggtaaa gttatagcta ttaataaaga
      541 ccaatacgat atggctccaa ataatgtaat aagttcaatt ataaaagatg tagagcaacc
      601 tgtctatgaa ctgataaaga atgttgtaaa agatggtttt aagggcaata aggtaatgga
      661 atttaaaata aaagatggag aacttggttt gacttcaaag agtagtagaa atataccacc
      721 agatgttttg gaatatataa aaaaagagta taacaaggtg aaagatacat attaattatt
      781 gatgtagaag attataaaaa tatactttat taagtttaga ttaactatat aaaattataa
      841 caataagtgt gtgggcagaa aaattctgct acacacttta tttatattca tataaaaaaa
      901 ttatcaaaaa aaagaaaaag gagtaaaaaa tggaatttac aagagaacca tttatactag
      961 atgacgattt gtgtctagct ttggtggaca aaagaattag ttatctaatg gaaaatgaat
     1021 taaaattaag aaatatagag ataataaaaa cagtagaatg taaagaatta tatgaaagca
     1081 taaaatatca cccagatata tgtatttgta atttaggaaa aggagatata attgttgcgc
     1141 cgaatgtata taatcaatac aaagagttat taagtagata taaatttaat ataattaagg
     1201 gagaagctaa tttattaaat agataccctt atgatatacc ttacaatatt gcgatagttg
     1261 gtgattatgc tatacacaat tttaagtata ctgacaaagc aatattagat tatattgaaa
     1321 ataataaact taaaaagata aatatagagc agggttattc taaatgctca atctgtatag
     1381 tagatagaac atctataata acatcagata aaggtatttg gaaaagtatg aagaatacag
     1441 aaatagagtg tttattgata gaaaaaggtc acataaattt atttgagatg aattatggat
     1501 ttataggtgg atgtacaggg ttgatttcta aggataaact agctttttgt ggagatgtta
     1561 aaaaacatcc tgactatgaa cgtataaaaa gttttgtaga aagtaaaaac aaggagatag
     1621 ttacacttag ctgtgaaaat ttactagata taggttctat agttcctctc atgacaagaa
     1681 aggagcgata actttggttg agaaggataa tatttcttcc atgaaatttt tgaggaataa
     1741 taatgtaaac gatagattaa tatatacgaa tgatattgta gaagtagtga tagattctgc
     1801 aaagcaagaa gtattaaata aaaaaatact taatggtact aaagctgaac aaagagcttt
     1861 aaatattgat gagatatcaa cggaaataac tagtatgata ataagtataa tgaaagagaa
     1921 acttttgaaa gaatacattc taaaatgcta tgggcgtaca tatcctgaag ataaaaataa
     1981 tatatacata tattcactga acatatttgc taaaaaagag atttttatga gagaaacagt
     2041 atttacgagg gtgagaaatt atatagaaga aaatgatttt ataaatgtag aaggttttat
     2101 tagatttaga atgagagaat ttatgaaata tatttctgct ataggtgata ttgcagttga
     2161 agagtattta ataaagaaag accaagatga atttataaga gttttaaaat actttataga
     2221 tactcaagaa gaaaagattg atttgcttaa agttcatata atggaagaca atacatttgt
     2281 tttatacgat aaaaatggaa ataagataga tagcatagat gatgaagaaa ttataaatat
     2341 ggtaataaga gaaaatttaa attatgagga ctttctaata agtaccttgt taactctatg
     2401 tcctaaaaaa atagaaatat tggatttatt aaataataat tgctcaaagg aaattataga
     2461 tactgtagaa gcaatttttg aaaataaagt tagtataatt atggaaaact agaatctagg
     2521 tgtataagag ttagccatat aaaactaaaa aaattctttt taaatcgaca caaaagactt
     2581 ataattgtat ataaagcata gtatagctag agctattaaa tataaagaat atctatttga
     2641 ttaatgcaat atagcttgca aatatacaat tgataaggag taattttata atggataatt
     2701 taattaggat actgatatta gctgttattg gtggatttat aggttatgtt acgaatgtag
     2761 ttgctataag actgatattt agaccaatag agcctattaa aatacctatt ttaaatatag
     2821 aaatagtagg tcttatacca aagagaaggg tagaaatagc agcaaatgta ggagaaataa
     2881 ttcaagaaga atttctttct atggatgaaa tacttgcaaa tatcattact gacgaagaca
     2941 aggaagaagt tgtaagatac ataaaagcaa gggtaaaaat tataatacat gagaaagttt
     3001 catttatccc aggtggtata aaaaacatga tacaagatta tctaggagag ataattgagt
     3061 ctgaagttaa gaaaagtata gatgaattga gtaaaaatat aataaataaa gcaaatgaac
     3121 gtatagatat acaaaaaatg gtcgaagata agattaatga actagattta tacgaattgg
     3181 aagagataat aataagaata gctaagaaag aattaaaaca tatagagttc ttggggcttg
     3241 tcttaggttt tttaataggt atagttcaag ggttagttac tatgtttatt taagtatatg
     3301 atacttattg actacattta ggtattaatt gtatttactt atttgattgt attacttatt
     3361 aactgtgctt aagtattaat tatatttatt tgactgtagt acctattaat tgtatttact
     3421 tatttaattg cgttgcttat taactgtatt taagtattta ctgtattact tattaactat
     3481 attaaatatt gattgtattt aagtatacaa aacctaaaag agtacaataa ttagaagtta
     3541 gttttaataa aaattagttt tagtatttat ctagtcatat ctatcatgca tgtggatatg
     3601 ttttaaaaat tttaaattta tgaaaatatt ataatatcac tataaatcaa gagattgatt
     3661 taatataaaa atataagaat attggtttac agatgattga gatgaaaaat aatgggtaat
     3721 taatatatta cattaatgtt gattgatgta taactatctg ttataatgaa aatatattaa
     3781 aaatcatagt gttgaagagg aagagtagag tagaaaccat tctcagagaa tttgggtcta
     3841 tggctgagag cccatgtaat aggtctattt gaaaactacc ttggagctga gctttattag
     3901 cagtatttgt atttatacta tatgtgaaag tgctcgggtg acgaccttat agtcatcaag
     3961 agggttgtat atacttgtat acgccaactt gggtggaacc gcgaattaat atcgtcccaa
     4021 atatatttgg ggcgattttt tattacaaaa aatctaaaaa ttatttcaat aaggaggaaa
     4081 aaacaatgat taaagttgct ttaaaagatg gctcaataaa agaatttgaa aatgctattt
     4141 ctgttatgga tgtagctaaa tccattagtg aaggattagc tagaaatgtg gtagcagcgt
     4201 ctgtgaacgg agaggttgta ggtctggacc acattataga tactgattgt gacttgaatt
     4261 tatttaagtt tgaagataaa gaaggtaagg aagtttttag acatacatca gcacatatat
     4321 tagctcaagc tataaaaaga ctttacccag aagcaaaact tgctatagga ccaagtatag
     4381 aaaatggatt ctactatgat atagatttag accatagatt agttccagaa gacttagaaa
     4441 aaatagaagc tgaaatgaaa aaaatagcta aagaagattt aaaaatagag agatttgagc
     4501 ttcctagaaa tgaagcttta gagcttatga aagaacaagg agaagattat aaggtagaac
     4561 taatttctga tttacctgaa agtgaaataa tatctttcta caagcaaggt gattttactg
     4621 atttatgtag aggaccacat ttaccatcta ctaaaaaagt taaagctgtt aaattacaaa
     4681 gtgtagcagg tgcatattgg cgtggtgacg aaaataataa gatgcttcaa agaatatatg
     4741 gaacttcttt tgagaaaaac aaagacttag aagaatattt acatttacta gaagaagcta
     4801 aaaagagaga ccacagaaaa ttaggtaaag aattaggatt atttatgata cctgaagaag
     4861 gtccaggatt ccctatgttt ttaccaaaag gtatggaact taaaaatgaa ttattaaaat
     4921 tctggagaga aatacacaga aaagctggat atattgagat agaatctcca atcatattaa
     4981 atagaaaatt atgggagact tcaggacact ggtatcatta caaagaaaat atgtatacag
     5041 ttaaaataga tgatgaggat tatgctataa aacctatgaa ctgtcctggt ggattaattt
     5101 actataactc tcaactacat tcatatagag atttcccaat gagagttgca gagcttggta
     5161 gagttcatag acatgagtta tctggtgcat tacaaggatt aatgagagtt agagcattta
     5221 ctcaagacga ttcacatata ttcatgttac cagagcaaat taaggacgaa ataaaaggag
     5281 tagctaacct aatagatggt atctacaaaa cttttggatt tgaatataat ctagaattat
     5341 ctacaagacc agagaactct atgggaagca acgaagagtg ggaagcagca gaaaatggat
     5401 taagagaagc tttagaagaa ttaggtttac catatactat taatgagggt gatggagcat
     5461 tctatggtcc taagatagac ttccatctaa aagattgttt aggaagaact tggcaatgtg
     5521 gtactatcca attagatatg caacttccaa gacaatttga taatacttat attggacaag
     5581 atggtgaaaa gcatagacca gttatgattc atagggttgc ttttggtagt atagaaagat
     5641 ttataggtat attaatagag cattatgctg gtaaattccc agtttggtta tctccaactc
     5701 aagttaagat acttcctata tctgataaat ttatggatta tgcaaatgaa gttaagaaag
     5761 aattatttga taagggtata agagttgagt tagatgatag agctgaaaaa attggattta
     5821 agataagaga agctcagctt gaaaaagttc catacatgtt aatagtaggt gaaaaagaag
     5881 tggctgataa taatgtttct gttagatcaa gagataaagg tgaaataggt agtattaaat
     5941 tagatgaatt tatagctagt atttcaaagg aaattgaatc aagagaaagt attattcaag
     6001 actagtatac aagatattag tagctaagat attattatat aagataaatt taatcaatat
     6061 gttgatatat agtgattaaa tttatgaaaa gatgagtata attagaggta cagttaatca
     6121 tatttttatg attaatgtac ctctagtttt tttgtatggt agatttatta ataaatatat
     6181 catagaagtg ttttgaatct ggaaatttta aataaaaact tattttttga gtgataatta
     6241 agtgcttgtt gataaggcta tggctgtaaa aatcataacc ttattttcat atttgttcca
     6301 ttttcatgaa aattattatc catttaagaa aatttcttaa tccttttgaa atatttcatt
     6361 tttagtctag acttaaattt ctttcttcaa aataaatgct taaatgaatt aaaatttagg
     6421 tttattttct aatcttatga gtctattttt cataaataat taccttataa tagaagaagt
     6481 tatgttgggg ttatgtgcta aaaataacag cattgtcaat gattaattaa tattttatgt
     6541 tatttttaga tttatcttct atacatatat taatttattt aactttagtt atagagagaa
     6601 tgtcgagtaa atacacagac attagagtct aagaaggtgt ttatggaaca agagttttta
     6661 aaagagatag ctaattagtt taatgtcaaa aaatgataaa tacaaaagag gaaaaagaga
     6721 ggtgtttaaa tgatagaaaa agtaggagaa aaatctaaga ttattaaaac atcaaaagaa
     6781 tataagcgaa ctataattga agaaaaagat agagaatgga ctcaaaaatt aaacgacata
     6841 cttagtagaa ataatttttc taatgatgaa ttagacaaaa tcaaggagtt aattccaaaa
     6901 gagattttga ctagtgaaaa tgtcggagaa gttgtgactg aagatggata tgctagacct
     6961 gaatatgatg aggtatttaa atcattgttc actttaaata atgaatacgg tttacttgta
     7021 aattttataa atgatattct aaaagatgct ccatatgcta acaggaatat caaaccgttt
     7081 actcaaataa agagaataat tagacttgaa actgacccaa caattaatta cattggagag
     7141 aaaaggccta gacttgatat tttggctgaa gatgaagaaa ataatcatat aaatattgag
     7201 atgcaaagag cttttgaaaa tgattatttg gaaagagctg aatattatct tagtcgtgta
     7261 catggtagaa agttagaaga aggtaaagaa tataaagaaa ttggaaaaac tattggaata
     7321 catatactaa atcatgtaaa atataatcac atagaagatt atgtaaattg cctgagatta
     7381 acaatggatg ggcatccaga gatttattct agtaaaactg cattatattt tatagagctt
     7441 ccaaaaattc ataaatctga ctatatagta aatagaatta tgctatgggg aaaacttatt
     7501 atcaatcctt ctagtttaga tgtaagggtt atagctaaga atgattctat tattaaaaaa
     7561 gcacttgata gattaaaaga attaggtagc aatgaagaat atttattaaa tctgaaaaga
     7621 ggtgcttata ttatgaataa aagtagaaat tttaaagaag agatagaaag agagacagca
     7681 attagagttg caaaagaaaa agattataaa ataattataa tgttgaaaaa aaatggtttt
     7741 aaattagatg atatattaaa caaatttaat gcttttgact taagcgaaga tgagataaaa
     7801 gaagtttatg aaaataatta atgtataaat aaaaagtgaa cttatacaag ttcattaaat
     7861 ctgagtctgg aaatatatat gtgtattttt taaataaaaa tttattttaa agaccataat
     7921 ttagaggtac agttaatcac actttcatga tactgtacct ctagtttttt tattgtaaat
     7981 ttattaataa atctacaata aaagtggttt tgttgtaata atcattatat ataatgatta
     8041 ttttttatag tgattaaatt ttcacttgta gtgcttaaat ataaatttgt aacactattt
     8101 ttataaatgt ataaatcact atgacgattc aactcaaagt cactgtacaa aacaccagaa
     8161 accttatatc aataagatgt tattgcacca gattgttcaa gtctacgaaa tgctgtatac
     8221 aaaatagctt ctttcaactc atacaatcct ttggtttttt caagtataga tttattaatc
     8281 tcataactat agctgtcttt tttaagtaaa tgttttaaga tacttgtttt tgtatcacat
     8341 cttataacat cagatgcgat tgtcatgaat atcacatccg attaaatata taatagtttt
     8401 atggactttg cttatcaagg tatatcatga aaaagacatt ttattgtaat atttataaga
     8461 ttttatatgg aaataatgta aaaactttat aaataatata ttttatacct agttatagat
     8521 aaatttatat agttattatt aaaaaaaaca ataaaaagtt taattcaatc tagcaaattt
     8581 agtgatataa ttaaaaaaga aaagaaatat ggatttattt ggaataattt tgacaatcta
     8641 tttactacac gtgtagagtc gtatataatt aaattataca taaaatataa aaaaataatt
     8701 tagtaatacg ttaggagaaa gtattatgat aaagtcaagc ttattttgga atattttcat
     8761 tgcgatatcc tttatgatag aatggtttct ttgtaagaaa atgctagatt atacaagtga
     8821 gaaaaagatt tctaacaaaa aaataaactt ttgtatagta ggtattattg tttttgctaa
     8881 cataatctat ttttttgatg cacctctaaa tataagagta attatatcta tgatagttac
     8941 tattgcattt tataaaattt catatgatgt taggtttata aagtctgtat taatatcctt
     9001 aatttattgg atgttactaa tagctataga tgctttgagt atatctttaa cagtttggct
     9061 taattctata aatgacatga gtaatatggg gacaggagat atatacagaa ttgaatctat
     9121 aatattagga aagatacttt tgatttcggc tttgttttta tatagagcct taaaaataaa
     9181 atttaatata gaaataaata aaaggacatt attttatata gttattccta tagtcgctaa
     9241 tatagcatct ttttttgtca tttttaaata catatttaat ctaaagtttg gtaacttaat
     9301 acataatttc caattcgttg tgatttctgt tgtattactt gtatcaagca tcactttgat
     9361 tttaattgct aagaaattac agcaatataa tactttagtt gtagaaaata atatgattaa
     9421 aaatactttg aaatgtgaaa atgagtatta tgaagatata gaaaagaaat atttaaaaac
     9481 tagattacta agtcatgata tgaaaaatca tatattgtgc ataaatgcta tgattaaaaa
     9541 aggtattgat gttacttctt atttaaataa tttacaggta gaaatccaga gtaatgactt
     9601 attatttaat actggtaatt ttatattaga tgctatatta agtgaaaaga aagaagtttg
     9661 tgataggaat gatatagaat taaaaatagg aataaatttt agttgttgtg aagctgttga
     9721 attagtagat atatgtcata tattttcaaa tattttagac aatgccattg aggcatgtaa
     9781 taaaattgat agtggaaata gagtgatttg tgttaaaggt gatattatgc aaaattttta
     9841 tctaataaaa gttgaaaata ctaagacgaa taagattatt actaaaaatg gagatatttt
     9901 gacagacaaa aaaggttctc ttcatggact tggaataaaa agtgtaaaaa actctgtaaa
     9961 taagtatggt gggaatgttg taattgaata tgatgataaa aaatttgtag ttaaaattgc
    10021 tattccactt agaaatgtga agaaagtggt taataatata atttaatata tgggagttca
    10081 atatatataa atttgatacg tagaagttta atgtaaaaat tttgtgtata ggaatttaac
    10141 atacatattt attttaatat atattgaagg tatataatta aatcatataa tgacatttgc
    10201 agtttgaaat tatttattga gtatgagaaa ttttcaaggt cagaaagttt atgactatta
    10261 tatattatat tgtttgtcgt atttattata gattatataa tgagtttaaa atattaaact
    10321 tttaatttta caaaatataa aaagaagttg catcaaaaag ataaatgctc tttaaagatg
    10381 taacttctat ggtatatgta tgaggagcta tattgccatt tctggaatca agcttctcat
    10441 agcttctata gttgtttgga taagcttatc aagttcccat ccaagcattt ctgcaccact
    10501 tcttatgaca tctcgagagc agccagcagc aaatgattta tctttaaatt tcttcttaac
    10561 agattttaat tctaaatcag aaacactctt tgaaggacgc attattgcag ctgcaccaat
    10621 taagccagta agttcgtcta cagcaaacaa tactttctcc ataaattgtt ctggtttgac
    10681 atctgaacca gttaatccat atccatgact tattgtagaa tcgattattg atttatctaa
    10741 atcaagctct gtcattaatt ctctttgttt aacacaatgt tcatcaggat acatttcaaa
    10801 atctaaatca tgtaaaagtc caacgatttc ccaaaattct actctttcag ggtcgtactc
    10861 ctttgcatag tatctcataa ctccagatac aatttgagca tgtcttatat gaaattcttc
    10921 cttattgtat tttcttaata tttcccatgc ttgttctaaa gttggcatgt aactcatttt
    10981 tatcactcct ctagctgaat attttgaatt tttataaagt ttattttcca ttgattatat
    11041 caatcaattt ccaaaacgtc aacatactga tacacaattt tgcagttaaa ttcagaaatt
    11101 agtgaaaatt aaatctttag gtctaaattc tttgtatcta aagtttttcg tttcttaatg
    11161 ttatctttga tataaatgct ataattaagg taataaatcc aactcctagt ataataatgt
    11221 acactctatc tgataataaa tcaaaaagtt taccatacat aacctgacca agaggcattg
    11281 caattgttgt tcctgcaaaa agaattgaca ttacttttcc taagagttca tttggagttt
    11341 ttaattgtat gattgttata gcatatattg agataagagt cgttgtaaaa aatattagca
    11401 tagatgatac gataaaaatt aaaaacggaa ctggaaatcc cgtatttaat ataaatggat
    11461 atactgaaaa tgccattggt atgaacataa gtgcacatat agtaaaggaa atgtacaaat
    11521 tagtgatttt tagatatttt gagaaaatac caatactaac agcagctact agagaactaa
    11581 aagaaattgc tccctctgct agaccatata agaagttact agctcccata gtgattttta
    11641 ctatatatgg tataccaacg attaacatag agcttaaaaa taagtttatt gctatagcta
    11701 aaaaaagtat ttttagtata aatctatttt ctttacatat atgaacaata ccatcattta
    11761 tatcaataaa tatagttttt ataatgttgc tggtttgctc ttgtttgata aaaggaatct
    11821 gtataaatat ctccataatt gcagatagaa agaaacttac acaacttata actataattg
    11881 cattaattcc aattacaccg tacaagacac ctcccaatac aggtcctgca atatttgtaa
    11941 gggatgcaac tccagctacg ataccatttg cattcattaa atgctcatta tcaactaaaa
    12001 gaggtgtact tgactgaaca gttggttgat acattgtgct tatgactgat aatatggtca
    12061 taataattcc tacaagtaat attgaaccat tattgttgaa taaaaagagt gctaagacta
    12121 acactgtaat actacttgaa aaatcaaaaa taaccattaa gtttcttctg ttgaatatat
    12181 cagcaatagc tccaccaata ggggagaata ttataattgg gattgaagat actgctagta
    12241 tagtagcaaa tatttctgct ttaccagtta tatcgagtat gtataaagac aaagcaaatt
    12301 taagtattga tgcaccaaat aatgagatta tttgacctaa tatcatcaag ttgaaatttt
    12361 tattaaaagt attcaaagat tttaccatat atatacctac ctatctaaga ttattaaaac
    12421 atttacaata attgctataa tagcaattac tataaggact ttttttctta ctttaatacc
    12481 taatattatt agaaatatag aaacgattat agataacata gagtaaagtg gcatgagctc
    12541 actgctaggg tctacaagtt ttgtgtggat atttagttta tcatttattt tgtaaataac
    12601 atcatttagt ctttgtatta attcaatcat attatccttt ctagttataa agaaatgttt
    12661 agaaaattta ttttaaatta ttactcaagt atatgttcta caagattgtc taccataaaa
    12721 tcaaatactt caatgtagtc atatccaata tcttctttta gcaataggct tgacagtagg
    12781 gcattgcaaa caccaaaggc tttttctttc tctattttta acttgagatt atgtctttca
    12841 attaagctat aaaaactttg tacattgttg tattcaagtt gtgaaagcat ctctttaggt
    12901 atcttattca ataaagtgat gtaatcagta ttatttggtg aatataagaa tgggttttta
    12961 tcatataatc gatacaacat ctttaaggct tttgcaaaac cttctttttg aggcatgtta
    13021 cctaatattt catctactga tgatataagt ctgttttgga ttccatttag tacttcaaaa
    13081 aataactttt cttttgaatc aaagaatgtg tagaatgtac cctttgcaat tcctgatttt
    13141 gaacaaagtt catcaatatt agtttttttg tatccatatt tagaccagtt gatttcacat
    13201 tctgaaatta gtctttttct tatattatct ttttctattt ctgtaaagct tttagccata
    13261 tttatcaatc ctctctgtat atacgtatta tctatgactt aaaatgtttt ttaagtcata
    13321 gatatatagt attgtttatt tataaatttg tcaagtgatt tatattatga gacttgtatt
    13381 aaaatttgat ttgaataagt ttcaagcgag tatgttatca aaatagaaaa gcgtagaaaa
    13441 aacgttaact ctaaaaaatt tttctaggta aacgtttata ataaaaagaa atgtttacat
    13501 aacgaataaa tggctataat ttaaatacac aaagttatat cagagagagg aataaaaata
    13561 tgtttaatga acttagaaca aaagaaaatg tatataaaaa tttaataaat ggaaaatggg
    13621 tagaatctaa ttcaagaaaa ccaatagaaa tttactcacc aatagataat tctcttgttg
    13681 ggaaagtaca atctatgaca aaacatgaag tagatgaggc tataaaaaat acaaaagaga
    13741 gtataaaagt atgggctgat atgcctgtat ataagagagc caatatattt cataaggctg
    13801 ctgatttatt gttagaaaat atagatgaaa tagctaatat actggtactt gaaatagcaa
    13861 aagacatgaa atctgcaagg gcagaagttg agagaacagc agacttttta agatatacag
    13921 ctgatgtagg aaaaaatatg gagggtgaag ctattagtgg agataatttc cctggtggaa
    13981 caagaaataa gatgtcttat gtttcaagag ttccattagg tactgtttta gctatatctc
    14041 cttttaatta tcctgtaaat ttatcaatgt caaagattgc tccagcactt attggtggaa
    14101 atgcagtagt attaaaacct gctacacaag gggctattag cgcacttcat gtggtagaaa
    14161 ttatgagaaa agctggtata ccagatggag tacttaatac tattacaggt aggggtagtg
    14221 agatagggga ttatgttgta acgcataaag gaattaattt tattaacttt actggtagta
    14281 ctgaagttgg tcagcatata tctaaaatat ctggaatggt gccattatta ttggaacttg
    14341 gtggaaaaga tgcggctata gtattagaag atgcagactt agattttgca gctaagaata
    14401 tagtgtctgg agcgtattca tattcaggac aaagatgtac agcagtaaag agaattttag
    14461 tacaggagag tgtagctgat aaattagtgg aaaaaataaa gccattggta gagaatttga
    14521 caattggaaa cccaaaagat gaagtagtta tcacaccact tatagataat aaagcaactg
    14581 actttgttca aggtctagtt gatgacgcat tacataaagg tgcaaagttg atagtaggta
    14641 atattagaaa aaataattta ttctacccaa cattgttaga taatgtaaat actgatatga
    14701 agattgcttg ggaagaacca tttggtccag tgcttccaat tataagagtt aaagatataa
    14761 gtcaagcaat tgaaattgct aaccaatcag agtatgggct tcaatcatca gtgtttacaa
    14821 gtgatattga tagggcattt tatatagctg ataagcttga ggttggtact gttcaaatca
    14881 ataataaaac tgaaagaggt ccagaccatt tcccattttt aggtgtaaag gcatctggta
    14941 tgggaactca gggagttaag tattcaatag aagctatgac tagaccaaag gcagtagtgg
    15001 taaatgttag aggattgtaa taaatagaga taaagaataa tagaaataaa aaataaagga
    15061 gttgtcttaa atttgatttt ttgtaattga attttaagtt caactccttt ttgaattttt
    15121 atatgctatt tcttagtaat tattttttaa taaatatctt aaactttgag attgatgaaa
    15181 tttgaacatg ataaatatct taaatttata gtcttgtttt ctaatgatga agcctatttc
    15241 tacttaaata gagcaatttc taaaatgaac atttttatct cgagtaccat atacaatgaa
    15301 aaaaattaac cttgacaagt gaccgatggt cgctgtatga taaataaaaa agaaacgacc
    15361 agtggtcgtt aaaaggaggt atcactctat gccaaaagga attgtgctta cacccgaaca
    15421 acaagcagaa cgccgagaaa aaatagttgc tgttgctttg agtctcatgg ctgaaaatgg
    15481 attccaaaaa acatcaatga gagaaattgc agttttagcc aacatgggaa agtcaagtct
    15541 atacgatttt ttcaaaacga aagacgagat tgttgtatac gcagttgaga aagaaataga
    15601 aaaaactatc caacaagttc ataggattat tgatgatgaa ctttcgccac aacagtgcct
    15661 tagaaaaatc atgcttaatc atcttagatt tccaaagcaa tatagaactg tactgatgtg
    15721 gttaaataca gaatctgatt atttagaaga agagtatcga aagagactta aagatgctcg
    15781 ttatgcatat caagatatta taaaatcggt aattgataat ggtgtgacca caggtatctt
    15841 tcgcaaaacg gataccgctc ttgtgacacg tttgttaatt aactccatgt tatcaatcag
    15901 ctacacgtca cgactatcgg atagttcgga aaaaatgttg gatgaaacaa tgaatatatt
    15961 tctgcacgga attatgaatg atggaggtga atgattatga tttattatgt tttaccacta
    16021 gaacataaac aagcaaccat tgaaattgtt ggtggaaaag gaatgtcttt atcaaaacta
    16081 ttgacagcag gcatccctgt gccagacggt tttcatgtta cgactgcttc ataccagatt
    16141 tttgttgaga cgaaccatat tcagtctcga attaataaac tgctagatgg tattgattcc
    16201 aataatacca gccaacttga agatgtatct aagaaaatag gggtgctttt ccataatgga
    16261 gaaatgccac aggaagtgtc ggacgcaatt aaaacagctt atgacggatt agggaacata
    16321 tcggtggctg tccgttcatc tgcgactgct gaagatttac ctgacgcttc ttttgcaggt
    16381 cagcaggaaa cttatcttaa catacaaggt gaggatgaag tcattgacgc tgtaaagaga
    16441 tgctgggctt ccttatggac agctcgtgct attgcttatc gtgtgaagaa taatataaag
    16501 cacgagattg tcgcacttgc tgttgtagta caaaagcttg cattttccga ttcatctggt
    16561 attatgttta cgttaaaccc aatcaatggt agacgtagcg aaatgattat taacgctgca
    16621 tgggggcttg gtgaagcggt ggtttctagc ttagttacac ccgatacaat cgtagtggat
    16681 aaggactccg aacgaattat atcgtatgaa gtggcaaata aagagattat gacggttcgt
    16741 acttcagaag gaacagaaga aaccatggtt cctgaaaggt tgagaaaaaa atatactctt
    16801 actcgtaatc aggtaatgca actgatacag cttggaaaga aaattgagaa gtattatcaa
    16861 atgcctatgg atgtagagtg ggcactggaa aaagataaat tgtacattgt tcaggctcgt
    16921 ccaattactg ttctgccacc ggaatgggta ttgccagaac aggatgttgt atacacaaag
    16981 ggaagtttgg cagaacactt accaaatcct gttacacctt tgttcgccac gcttggtctt
    17041 gaaatcgtaa accgtgcatc ggagctttta tggatagata tgtttggtaa aagtgcgaaa
    17101 aaactactgc caaaaaacgg ggcatacacg gttattaatg gatatgttta tctttctgct
    17161 aaatcaaagc catttttgat tgctatcaaa tccctttccc cacgctcttt acgtaggaca
    17221 cttatgaaca gcgttgcacg ctgggaaacg gcacgaaaag gatatgagga tgctattaaa
    17281 caatgggaag aaaagcctct gcatacgatg aacgcacatc aaattatgga gggtattcag
    17341 acaatatttt atgccgcatg tatttatttc acaaggattc agcttacgtt gcctgctgct
    17401 tctatcagtg aaacattatt tacaaaatta tttcaaggag cagttcgccg tgctggtata
    17461 acggatactt ccgttttcct gcttgggttt gatacgattg ctctgcaatc ggaaaaggag
    17521 ttatggggtc tttcagaatg ggtaaagcaa aataacagca tcaaccttta tctgcaaaac
    17581 agtcctgcaa taaagatagc agaagatttc atgtcttcag ttgtgcctgt tggagtttca
    17641 caggaagtat ggatagaatg gaaaaatcga attaataagt actttaaaga gtttggtcgt
    17701 accgcttatg aatttgattt tgcgtattcc acaccacagg agacacttac accaactttt
    17761 gaatccgtaa aaacatttgt ggaaggaaaa ggggaaagtc cttttttacg ccagaccacg
    17821 tttgaaaagc gtagaaaaca tgcagaagaa gagattttac aacacatagg tggattgcgt
    17881 aaaaaactat tttttaagtt gctccattgg gcgcaggaaa cctctcctat gcgtgaaaat
    17941 gctatttatt tgatgggaat gggacatcct ctgattcgct gtatgtttca ggaaatctca
    18001 gaacgattca tacgaggagg agctatttca catttagatg atatttattg gctcacaaaa
    18061 tcggaattgg aggcgcttat agagcagttg gataaaaata agtctctatc tgatatgaac
    18121 gggttaatac ctgttcgaaa agctgaactt aaaaaataca tgggttatgt gtcaccatcc
    18181 aagttgccag agaaaaacaa aaaaactata tcacaagcac tacagaagca gaaagatggt
    18241 aaaattgtgt tgacgggtat tggtaccagt actggtgttg tgacggctcc agcttgtgta
    18301 ctgaatagtc cagcagattt tgaaagcttt caacctggaa gtgttttggt tgcagttacc
    18361 acaactccag catggacacc attattttca tctgcaagtg cgattgtaac ggacattggt
    18421 ggtcctctta gccattcaag cattgtcgca cgtgaatatg gcattccagc cgttatggct
    18481 acacacattg ccacacgaac tatcaagagt ggacaaatga taacggtgga cgggttggca
    18541 ggtacggtga cgtttgatga gtaaaaagcc tattttcgct ttgaagcttt caacctttgt
    18601 gattgcgtta gcggaagtag taatacttaa agttttgaga tttaatcata ctactaatac
    18661 ccaccaaaca gcggtaagaa aaaccatagc tcggtgggta ttttcttatg caaaaaaatg
    18721 agagtgtatc aaaataaaaa tttgattaga aaaaatcaat tttgagacaa ttcctttatt
    18781 tgttaataca ataatatcac ttataataag caattttata ttgttaattg gaggagtttt
    18841 gaaacataag tttttcaaaa tcttttattg gtaaaggtct agagaacacg tatccttgta
    18901 acatatcaca gtttatttct tgcaagaaat ctttttgaaa actggtctct attccctcag
    18961 ctacagtggt catttttaac tttttagcca gttcgattat agatacaatt atatctcttt
    19021 cacgttgaga atctaaatgt tctgattgaa aaaatgctct atctaatttt aatgtatcta
    19081 cataaatatc cttaagcata ttgattgaag aatagccact tccaaaatca tcaatagagc
    19141 atttaaagcc atttttatga atttcattta ttatttcact aaatactttg acatcttcaa
    19201 atacaattgt ttctgtaatc tcaatttcta taaggtgaga aggaatatca tattttttag
    19261 ctattttatc atatgtcttg atgaaattat tggatgaaaa atgataacga gacatattta
    19321 ctgaaatatt tataggattt aatccatcat caatccattt ttttaaagtc ttgcaaactt
    19381 cctcaaaaac atataaatcc aactgtataa taaatccatt tgtctcaaat aatggaataa
    19441 attcgtttgg aggaattatt ccttcaacag gatgaatcca tcttactaaa gcttctgcac
    19501 cacatatggt ttgggtagat aactccaact tgggttgcaa ataaacttga aattctcttt
    19561 tttcaagtgc ttcatacatt ctattttcta tgctttgttc tagaataagt ttttccttat
    19621 cttttttact atagaaatta caaatacata aaccatgatt ttctacgttt ttaattgatt
    19681 tagaagctac atttgcacta tcttggatat ttgttatatc taaagaagta tcataaatta
    19741 catatgcacc agcagtaaaa taaagtatat atttataatc tgcgttatcg ttgaacctat
    19801 taatatgtgt aaccattgag ttcagtctag aagttatatc ttctggttta tcttttaaaa
    19861 gaagaataaa tttatcagca aagattctgg taaccaattc accctcatgt aagtgtttac
    19921 ttatacagtt gtagatatat tttaaaagct tatctccagc tatatttcct gctaaatcat
    19981 ttattaattt aaatttttgc atgttcaacc atattagagt atatgtaccc gatggtgatt
    20041 gactaataac ttcttttgct attatattaa actttgtatg attcatacca ccagtaacgg
    20101 agtcaataaa agctaatttc tctaattctt tattattttt ttcaataatc tcattttgtt
    20161 ttactaatgt tgttacatct gaatatatta aaagatatat aggtttacca tcttcccaat
    20221 ctatacattc agctacaact tgcatatgaa caatatcccc attgatattt tgtggagaaa
    20281 cagaaaaaca aacgtatttc ccagctttaa aatcactttg atatttgtac acagcactaa
    20341 gataactttc taaatttata tcatctaata gaaaatttac attatctttg aacatgtcaa
    20401 agtatttttt attagcatca agaagttgga atccaaagtc agtaacctta aacttggcaa
    20461 tgagacctgg aaaattgttg taagttacag tttgttctat tttaatcatc atttgttctg
    20521 taatatcaat cataacagag tatgaaatag gatatccatc aacgacttct cctgttaaaa
    20581 atccaaataa ctttatccat aatttttttc cattacggtg tttcatacga cagacatagt
    20641 catatttttt tgctccggtg gagaatgtct tttctacata ctctacaagt tcattccaat
    20701 cctctggatt atccttgtaa aagaggtcgc attgattgtg aaatagagtt tcgtactctt
    20761 cttttgtata tccgaacatt tcataataac ggtcattagc ccaaacaaca gtaaagtgtt
    20821 catctaatag gtgtttacta acacttacat ccagtgcatt cattaataaa ctgtattcaa
    20881 tatttgcaaa atctatatat tttgacattc ttagtactcc cttataacat atattttgta
    20941 gaatatctga actagctagt ttaaagtaga tttattttta agcttatttt catacatcaa
    21001 agaatctgcc tctgctatta aagtttcaat atcggatatt ccatcccact caactaatcc
    21061 atagcttatg gaaagagcat aaggatactc gtcggtatta cttaattttg ataattcata
    21121 atttatatca atcattattt tttctgcttc tatacaactt gtatcctgta aaaggaataa
    21181 aaattcatct ccaccatatc taaacagtaa ggaattttct acatttataa gtttttttat
    21241 tatttttgca gtagtggata tgtatttatc gccaaattgg tgtccaaagt tatcatttac
    21301 aagttttaat ctgtttaaat cggtaaaaca taaagtaaat ttgtagtgat ttgacatcca
    21361 ttctgatagt ttttgattta caaaaggtct attgtagaag cctgtcagta aatctgtata
    21421 tacaatatca ttcaatttat taatttcatg ttctgttttt atgctttcta aatattctag
    21481 ttggttttta atatcataac tttttatttg agtaattcta aaactatatt tttctgcaat
    21541 atagttacct accaaaacta ttatggcaga cttaattcca tataatttta aaatcaatac
    21601 atttccaaca gtataatata ttgatagact taaacataga tacaagtaca ttacaaacat
    21661 aaggcttaat aaaaaattta gatttttttt gtcctctaaa actgtgtcaa aattaatctg
    21721 attattttta tgatttttat aacagaaata tagacctgta actataaagc tagatgatat
    21781 agaatatact cggtaaatat ttgtaatgta attactgtca aataaatatg ctggcatatc
    21841 taatgttatt gacattaaag acctaaaaaa tatgtttatt gccaaaccaa taatagaaca
    21901 gtttagtgaa taaaaaagat tttttattat agaggttttg taagttattt taatttctat
    21961 gaataaaatc aacgtaaaaa tcgtccaatt tataattaga tgtaacctta aataagtaca
    22021 aataataaaa attaaataat taaacagaga gtatataata tatactttat atttattttt
    22081 agagttacta atcattatat cgagatattt aaaaaagtta taatgataaa ggtaataaga
    22141 taaaaaacat attaaaatta taagtaatct catatacata tccattttag ttactgttcc
    22201 ttttatgatt attttgttta ttagtgtaca tcctaatatc agcaatatta ataatttctt
    22261 ctacagtcat tttgttttcg ctaggaactt ccacaatacc atagctaaaa gatatcttat
    22321 tatacatact tttacgtatt ttctctataa attcttttgt agtgttttca ttagatttag
    22381 gcataataac tataaactca tctcctccta ttctggcgaa tatatcatct gcttttaaat
    22441 ttgaaataat tatacttgaa aagttaataa tatagttatc accagctaaa tgtccatatt
    22501 tatcatttat tgtctttaat ttgtctaaat caatatatat caatgaaaat aataaactat
    22561 tatcaatgta atgttgtatt ttttcaaaag caaattttct attatatgct gatgttaatt
    22621 catcaatgtt tgcttttttc tttaataaat tgacttttaa taattgttct tgctttatca
    22681 tttttagtct attatgttct ttttctaaat aaatattttt ttcaatggaa tatatatgat
    22741 tgatgaatat aatcatttgt aatgataata atatgttact tcctaatata aaataagaag
    22801 ttaaagcgct tggtggagaa aatgcaattt gtatactatc gaataatata tatcctaaag
    22861 tgaagtagat aaactttaaa aatagactaa attctatttg attttgagtt attacataaa
    22921 tatgctcttt tattttatcc atagagtacc ataagataaa atctaaaata gaagctatta
    22981 aaatactaat tatcctagat gacatattta caaatattat atctggactt gtattttcga
    23041 caattgacat tatacttata gtaataagat gtaaaataca aaaatttatt actgctatat
    23101 taggaataaa gtgtttgtta tcagaatttt ttatgctaat taaaagcaat ttatgactta
    23161 ctatatagaa caatatataa aatagtggta atggcaattt tgtgaatatt attgttaaga
    23221 aagttagtgg ttttattaaa aaaatatatt tataattctt atgtatagct atacttttat
    23281 ttccaaaaga tgctatcttg tagatgtaaa tattacatag ttcaactatt aatatgatag
    23341 ttatgattaa aaatatgtgg tatttcatag tacatcctct tttaaaatga aattctattt
    23401 acttaaaata gattagattt tatatactct attttacagt ttttatttaa atacatgtaa
    23461 agaacttatg gatatttttt tattgtgtat gattgtgtta gataaagttt gaaaaatatc
    23521 tctatatctt acatttatac atagtaagta tgataattat acagtaagta ttatatgatt
    23581 tattatgtaa aataagtaga gttattctat caataaatat acataatatg ctaaatatat
    23641 atatttttta ctgaaaattt gaaatactta aaattgttcc agttaataca ctacaataat
    23701 atgttaactt attgtagtat gactatagta tgctatttta ctgcattaca gtattagaaa
    23761 ccttagacta cggatataaa tggtttatat taattataga ttatgaagaa aaaataagca
    23821 actattttgc tttattgaat aaaagtaagt ataacttaca aaaaataata aatttatttt
    23881 actaaatagg aaaaatataa agaaaaaaac taaaatttga ttatttttga acatataatt
    23941 aaacaaattt atattggtaa atttacacac agaagataga ctcgattcta taaaatttgc
    24001 tcttaaatga cagggaatat gccacaatta ctattgagga gatagtactt ttactatgga
    24061 ataagataga ttatatagct gtagataaaa taaaaattgt ttaagacgtt tacaaaataa
    24121 ttgtgaggag tgtaaggttt atctagactt tgttttgtaa aatagcagat tatacaagaa
    24181 tttatatgtg aataaattta ggagtactat aaaaaataat aaagttttta tatgatttta
    24241 aataaattaa gttaagtaat ggatattttc taagtataat aattgaaaat atccattgtt
    24301 gtatttatta tattttaaaa ataaaatatg ttttgtattg tagaaaaatc ttgttatcta
    24361 taaaattaag ttattttata agtattttta tccagttata cgtatttatt gaccagttat
    24421 acaaaaatat tgaatattaa gaaaattagt actactatat aaataaatat atatatataa
    24481 aggaggtttt ttatatgagt cttattgttg gtggatggtc aaaatttagg gctgtcgaag
    24541 aagaagattt aaaagtattt aatgaagctg taggaatgtt aaaaggggtt gattatgaac
    24601 cacttattgt tgctactcaa attgtagcag gaacaaattt taaatttatt tgtaatgcta
    24661 cttcagttac aaatcctccg catgattact tagcagaaat aattattttt aaccctcttc
    24721 cttgtgataa acaaaaagca acaattacaa gtattaatat agttaagtaa tattaatagg
    24781 agctggaata tgagattaaa tataactaaa caaaatacga tagaggttga aggtagtaaa
    24841 aaagaagatg tttattttat aatagttgat aatgtttttt ttgaacgcaa aggtaatgta
    24901 ttctatagta tcacaaataa taatttaaat aattatgata taaaaatatt accaactgat
    24961 aatatttttc tagaattaaa aataacatct actaaagatt tagaacctgt ttttattgta
    25021 gctgaagata gacaatacaa tcattgcagt gtaatgttta aaccagtaat ggtgtagtgt
    25081 ttaatcaaat aaaattaaaa tattcaggct agtaatggtg tgatatttaa tcaaataaaa
    25141 ttaagatatt caacccaata gcaatgtaat gtttaattta attgtagaaa tataatttta
    25201 ataagaaagt ctatatttat aaatagagca aaatagtgaa taaataatat aaaaacaact
    25261 gtagtacata aagtacaata aaataagttc attaactaaa ataactgtgt tgataaattt
    25321 tatattatac aacacagtta ttttaatgaa tagaaataaa taattataaa atgaaaatat
    25381 aaacagatta aaaacaaaaa ataaaaacaa atttaaatac agtctaaaaa ctttacaatt
    25441 tacctaagtt attaaacttt atttaaataa gaatatataa gcaaggaggc aaatgaatgc
    25501 cagaagataa taaaacattt ttatatgaat taaaagagtt attaagtaac ggagaaaaac
    25561 aaatagctaa agcaataggt caagatttaa ttttaattct tggtggaaca ggctgtggaa
    25621 aaagtactgc aatcaattat cttgcaggat gtgaaatgca taaagtagaa gatgaagata
    25681 ctggagtatc ttacattacc tgcgaagacc ctgtagcaga tattggttca ggagctgttt
    25741 ctaaaacttt atatccagag gttatagata tgaaaaaagg ctttgaagga atgactgcaa
    25801 aattttgcga tactgcagga tttggtgata acagaggagc aatccatgat atttgtgcag
    25861 cagtttcatt aggtgaagta tttaaagatt ctgcatctat atgtgcagta attgtcttga
    25921 taccagaagc agatatatta gattctagac ttacaaaggt actagacttg ttccaacagt
    25981 taaatatgtg tcttcataaa gaaaatttta agaatagtat aacttttttc atttcaaaga
    26041 gtttcttagg aagaacagaa aaacaaatat ataatgttat aaaaagaaaa tatgaacagt
    26101 tatgtgaagc ttcagatagt aatatgtcag attcaaatag taatataaaa gaaaaagctt
    26161 ggttgtttga agaaatgcta aaagatgatg gcaaaaatat acatatttgt aatccgcttg
    26221 catcagatga tagggaaggc ttattaagac aagtactaga attagaggaa gttaaagata
    26281 agatatcagc attccaatat ccaattagtg cagatgcact attagtttta gatacgttga
    26341 taaaagaaat tcaagacaat atcgtatcta gcttagaaga ttatgttaag gcttatacag
    26401 aagattttat cagtgctgtt ggtagtataa caatgattga agaaatagca aaatatgatg
    26461 atgtagtaga agtatttaaa aactggttgg aaagtacaac aagtccaact ataggtagat
    26521 ttatcaacga attagccaga caaagcagtt tattcttaca agatagttgc atgaaattac
    26581 aaaagaatat aaaagaaaat ttagatataa ttgatgcttt aagtgaatat acagaacatg
    26641 gaactagtga aatcttatta tcttctgtac atatggaatt tattatagaa aagacaagta
    26701 agaagataat ggaacaaaac ttcattataa taagaaagaa tttagaaaaa gttttagttt
    26761 cttatgaagt gcaaaaacag gtatttacat taccaaatag agaaaatcta atggaagttg
    26821 caaataaaaa ggattataaa tatagtgtaa atgattttaa ttcaattttg acaaatgtta
    26881 cagttacaca aaaagaagag ttagaagaca gtataacaag attaaatgca tatggagaag
    26941 aaggtaatga aataattaga gaatttatta gtaaatacat tttaaatcca tttatagtaa
    27001 aaacaattaa tattaatgat aagcaaggta ttgaaataag agcgttaatt ccaaacttag
    27061 taattagtag tgtattggaa tatttcaaaa atagaaaagt ggatgacaat gtttctacat
    27121 ttatgttctt tgctaaaaat actatttata tggatgataa ccttggaatg gactttgcat
    27181 cagagaaaaa tattattatt gcttgtaata atctcgacgt aggtagtaat gtaaaaatta
    27241 atgtaagtgg aaagagtgga gaacttgtag ataattctat gtatgggaaa aatggtgaag
    27301 atggtagaga ttcaggtaat atttacttat atattaaaga agaaattaga aattatcgtt
    27361 taacactgat tgcaaatgga ggaaatggtt ctaaaggaat agatgggaaa aatggtgaaa
    27421 ctggagcttc aggaagtaat ggagctgatg ctggatatac aagtagtttc cacggaaaga
    27481 tgtttaaagg gtattttgca caaggtacaa gtggtggaaa aggtgaaaaa ggtggcgctg
    27541 gtggaaatgc tggaagaggc ggaaatgctg gtaagaaagg taatatttac ttcatttcag
    27601 attcagaaga acagaaaaat agaataatta atcgttgtga tataacagtg agagatggag
    27661 aagtaggtcc aaatggtaga ccaggttcag gtggatatgg aggaaatggt ggttatcacg
    27721 gatataatac actttcattt tctccaagtg gtatagataa gactgtatac tatacaggat
    27781 actttacaag ttgggattat aagttagata gtataattaa atatcatata gaatttagtg
    27841 gtggaagttg gaatgaaggt aaaacaggac caggaagaga atacgctgaa tatggagaca
    27901 gaggagcaga tggaggattt aatgctccaa acactaagat atcaacagct gtttcaaaaa
    27961 tagaagataa ttttattaaa gaaaaatatc aatgctattg ttctgaaata tagaaaggga
    28021 tagatatatg gcaaataaac tttatagtgg aattgtaaac cttttggaag atggaaggga
    28081 cgagcttaga aaatataatt tagaaaagaa gtctatttta ttatttttag gtgcttcagg
    28141 tgtcggaaag agtacttgta taaattattt aaaaggttgt gtaatggaag aaaaaacaga
    28201 tgcagaaact gggcaagtat atataactgc aaaagattcg gctgtcgaaa ttggaaatgg
    28261 tgtatattct aagactttat gcccagaagt tgtagatatt gcaaataggg atttttcatt
    28321 atgtgattgc ccaggttttt ttgataaccg tggagcagag tatatgattg caggtgctat
    28381 gcttgtaagg gaaaccatct ctacatcttc aaaggtgaaa gggatggtag ttattttaga
    28441 ctatcaatcc ctattagact ctagatatac attattaata gaaacgggca aaaatttgaa
    28501 agatatgctt ggtgattatg caaagtataa agaaaatttc ttctttctta tcaataaaat
    28561 acctaaagca ctaataagca atgtaacaga agaaaaaata agaaatatat tgaataaaac
    28621 aaaagaagat ttagatggac aagagataga ttatgatgga tgtaaggaat tgatttactt
    28681 attggatgta atgttatcag atagtgtatc tatagaattg tgtaacccac tagataaaaa
    28741 tccagaaaag ttattggata aaatgaaaaa tttacctgaa acttttgaaa aaacaaatct
    28801 gggattgatg cttactgctt cttgtaaaat agagatacaa aaagctgttg atgtaacata
    28861 caaagagtta gaaaaacttt tagtccctta tatagaaagt gtcaaaaaag gtatggaaga
    28921 ttctatttcc actctatctt ctataaatag tctaaaggat aaaaaaattc aattagacag
    28981 gatgtatacg gatttatgtg gtagagctaa tagtatgaaa gagcttatgg agtttgtaca
    29041 agaactttca tatggtgatg gtatgcagaa agtgaatata ttagaagaaa cacgtcttct
    29101 tttagagtta aatagctatt gcagtgagac ccatattgtt ggtctaagtg agttgttaaa
    29161 atcattacta aaaaatactt tagaagatac gttaaaagat attaaatatt ggatagcaga
    29221 ggaagaatat aaggaagaag aagccaaaaa gcttgaagat gcagaatata ggatagagaa
    29281 gattcaaagt tgtacatata taaaatcata tgagaaagaa aatgtacttt ctatatatat
    29341 tcctatacct aaagtgaatt tatcagattt gattaaaata atatctggtt ataaacttga
    29401 tattttatca tctatatata tgtatggaga ttgcgaatta aaaattgatt gtagtatgga
    29461 gaaagacgtg ttttcaggta aaaatattgt attgcttaca gagaaagtat ttataccaga
    29521 aggctcttat tcaatcaatg tatctggtaa gaatgcggag aagttggctg gagaagatta
    29581 tatatacaat ggtatgaatg gaagaaatgg taataatgca ggtacttcag gtgatattat
    29641 attatctttt aaagaaatag aaaaaatagg agaacttaaa ctttatgtag atggtggacg
    29701 tggtggaaat ggacaagatg gaaaaatagg agaaacaggg ctaactggaa aagctgggga
    29761 aaacgctaaa ataggttcta aaaaagagtc aaaagaattt gctaaggata aagatacaaa
    29821 atactatttg gtaaaagcag aaaaaggaaa acaaggtgga actggaggta taggaggtaa
    29881 tggtggaaat gcaggtcttg gaagcaaggc tggacaagtt tatctccttg gaaaagctac
    29941 tttattaaaa ggtgctataa gtaagaaaga tgggaatcac ggtttagcag gaaaaggagg
    30001 ctctggtggt gctggtggtg caggaggtct tggtggaatt agaggtatct atgaagaaac
    30061 aataagatat ataggcatct ctgctccaat agatgatagt gatagaaact actattattt
    30121 tgatagatat gtgtttaatc atgcttcaga tgagaaggaa gtagcagatg atggagtgaa
    30181 attaaatgct ctttatgaaa aacataagga ctggacaatt agcaaacgtg aagattcagg
    30241 ttcagtcgga gcagagggaa aagacggtct ctctggagat acattaaaag aaaaggaact
    30301 gttaaagcaa gaacattctt tgtcaataga tacattcttt tatgaatttt tcgagagatt
    30361 tggtttttct ggaagtaaag gttatagaaa tcttagactg ttgtgtgcag taggaatttt
    30421 agaagcggat attttagaac tttcacatat ttatgaaatt tttcatgcag ttgagaaaat
    30481 agagatgcag ttaaatagag aaatccttca gaaatactat ggttttgaga agaaaaataa
    30541 agcagtactt tctctggatg atatgaatat aaagaaagaa ttttataaag aggcaaagga
    30601 aatatatgaa taccttataa ataaattgat gcagtctgat agaatagaat ctttcatagg
    30661 agaaaagcag aattttagtg aatggagtat gattttattt gcacttgaac aaaaaacagc
    30721 attctatgtg cagatgatag aagaaagtga aattggagga aatcgtgtta cagatattcg
    30781 ttctttgaca gattatctgg ataagatctt aaaggagttg gaagaaggaa agaaagatga
    30841 gttaaaagaa gggtatttca atacatataa ggaaagtatc ctaactcaaa ttgaatctgg
    30901 aaatcaacaa atagctgttt taaaaaatga acttgagaat aaaatgcttt ctgttaatgc
    30961 aaaaatgaac ttgttgattc aagaaattat aaagataaaa gaagatgaaa aagaagatat
    31021 agagaaacta aaagagaaac aggcaaaact tgaggaacaa atgaaaacac aattaattct
    31081 ttctggaata caaagcgcat tgagtttatt gactgcattt agtggcgttg gtcaagcttg
    31141 tttaggagca tttgacagtg caaaatcttt gaaggaaaat ctttcagcta aggaagagga
    31201 ataccaatgg actaacagga tggttgattt ttcagatttt aaattggatt ctgatggtat
    31261 gagatttgta cttggagaac agttaagtga agatagaaaa aatgctttag ataaaatgac
    31321 ttataacttg gcaggtgttg atgaaggaaa aagagataaa tttgaccaaa ttaaggagat
    31381 tatagttaag tctatagatg atgataacta ttcagacaaa gatgacgata ttcaaagagc
    31441 gaaagatggg ttaagtgaaa gtgagtatga ggaaatttac acaaaaatcc agaaaaaaga
    31501 aataggtttg ttggaagcaa attacaataa taaacaacaa aaagacaagt tagctgcact
    31561 agagaaaagt aaacaaacta ttgaaagaca acaaaagaca tctaaaatta aaaatgcatt
    31621 gagtattgga agttctgctt gttctcttat aaatactgca attagtggtg ttaataattg
    31681 tcttaaagta aaatctgact ttgaaagcaa aatagaggta attaaagatg caattaaggg
    31741 taaggaagat aatattaaga aactagatga attgaatact caaatggtgg agtttaaaga
    31801 taaaactttg aaggaatctg ttataccatt tgtagaaaaa tttgatagta cagttaaaaa
    31861 tcaagatatt tttgaaaatg agataagtaa attaaagatg aaggaagttt taactgaatt
    31921 aattacaaag tttggaagtt taaaaatgga aaattcagag gagatttcta taatatttaa
    31981 aaaagtacag gatatgatgg aaactcaaat tattattact gataagattg ataagaagaa
    32041 agataatctt gcaatgggag aattgattta tcaaatgtca ggtagtggta gtcaaatgaa
    32101 tgaatatcag aaaaaactta tccatcaaat tgaattaaat caggttagat atttgtgtca
    32161 ggtggaggca gcagcttttc aactatggac atttccattt ggaagcaata tagtaaacat
    32221 attaaataga tgtcatgtag ggattacaga agatgaaaac gaggttgtaa atcaggcgaa
    32281 agagcaaaat agacagatta aagagtttct tgaccaagat gcttatcaat ggcaaaaaac
    32341 ggataaccat attttgaagg tagcattagc aggaagttta gatgggaagt cacgtgtggg
    32401 gtttgaaaat acatattcta tatatgactg taacgaagaa gaagtgaaaa agcttttgac
    32461 aggagatgaa attagttttg aggttccact aaatcaaaac tttgatgcta taaaatatgt
    32521 aactatgtat gcaaatatcc caaatcttag acgaaatatc ttagatgata gtttaaaagt
    32581 aaaggtgcat attcatttat atgatagtgg atacttctct ttatatgatg ataaaaaaga
    32641 tacctattat caatatgtat ttccacaaaa ctatatagac attacacatg gtcttgatgt
    32701 taaatacaat gattcagatt caataaactc aatatggtct tcggtggaag ataaaggaat
    32761 tgaaaagttc aaaaaagcag gattaaactc tggtagaagt ccatattcta tggctaagat
    32821 ttctttgaag gcacatttta ataagaagct ttcttatgat ttacttacaa atgagcagaa
    32881 gaagaaacta gaatcatact ttgataaatc tagtaagtat gcatggagcg agagtataga
    32941 tatattgaag agtctagggt atattgattt gaagggaatt ttgtcaaaga gttggtatga
    33001 aatcaagaaa gatatagata gtgatggaga gattatgaag catttttcta tattacaagt
    33061 taatttattg gaaagaatta tttatgaaca gactagtata ttaaaggaaa atatacctaa
    33121 tttgacaatt gaattggtag gctgtggtat ttatgtagag tctgaattta attatctaaa
    33181 gcaaaatgat ttaaaatgct atgaagaaag attggttgaa aatacgatta ttgattgaaa
    33241 atataactaa tagataagtt ttaaaagaat ttacaaaagg agatttttca aaatgaaaat
    33301 ttgaattatc tccttttttg taaaaaatag aaagaagttg agtttaaaat ttgtagaata
    33361 tgttttatat aattttgatt agaaaaaaac tacttttaag acatcttctt tttaaaggat
    33421 gaattataaa ataaaatagt atttgcacat gttacacctt caaggaattg tataggctaa
    33481 tatgctttat aaaaagccgt atgaaactat tgtgtttgct agatgttcaa gtttactctc
    33541 gtcaggagat tctcattttt cggatttctc aatcatagtt actatttgga gaaatctagt
    33601 agcatttgct tttccttgag acacataaca atattgtatt attctattac agtttcagaa
    33661 tatatgtaag agccttcttt tttgttgact tcaagaaatt cactgtgtca aattgtatat
    33721 agattttttt ctaatacatt cttctaataa ttagtcaata ccaataagaa acatgactga
    33781 accagccata tttatatcct ttctaaaaat agaaatacct atagcagagg catctaaaat
    33841 agaaatttct aattttttat ttagtagaga agagagacct tttttaaaat attttataga
    33901 gttaagtgcg attataaaaa ttttaagtac tttgggaaac atattttgga tatacttttg
    33961 attagtagtt tatttactaa tttgtctctg taataattgt tattaataaa tttataaaat
    34021 tatatgacat ttataccagt atatattaga attaaagaaa gttaatttat agttctttgt
    34081 tttaagttaa gaaaccgagc taagcaattt aaaatatatg gaatggagaa gaagtatgaa
    34141 taaaaacgac aatttggtta ttatatgtat gtttataggc atgatacttg gagtggctat
    34201 aggttatgtt atgggtattt ataaaggaag tgtaggtacc acaatgtgtt atggactagt
    34261 ttttggaatg ctaattggca tatgtatagg ggctgttatc aaaaattcta ataaaaaaga
    34321 ataaataaaa ctttatttac taggatattt agttacaatt tatttatgat aaaatttttt
    34381 acgtatatgc aaaaaatcaa ataaggcata ataacagata tttttattat tatttttcta
    34441 catagaaaga attgaattta tatacactac atttaaatta taaaaaataa aagttaattt
    34501 tgtttaggtg gagctttttt tgctcttata tattgaataa tgtcaagtaa gtctgcatct
    34561 tctaaaatac gacacctaat ccaacctcca ttattaccac aaggataacg agtgacccag
    34621 tgttctttag cttttggtgt caaagatatt aacatttttt ctactgctgg aacttgcttg
    34681 tcacctattt gaatcataac agtgaatgca cctttttcaa aaaaagcata aaataaatga
    34741 aaagatttat ggctatattt gtaaccccaa ccataattat ttccaaaagg aaatcgaagt
    34801 tctcttgata aattatattg agtctccaag taagtttcca acttacacaa gcgtgcatat
    34861 ccatttttac ctatatattc tattatttga gacaagcaag gtttattgct tttatcaagc
    34921 atacgctcat acattagaat ttacctccgt ttttactttt aagtatagca aagtaattat
    34981 gacaacagta tgtcaagttt ttatgtctga ttatactaat atttaaaaat ataaagtaat
    35041 tagttttagc taatataaaa atatgatatt tatatatgtt ggaaaaaaat gtaaaataat
//
LOCUS       DAFNNB010000022        34198 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0022, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000022 DAFNNB010000000
VERSION     DAFNNB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 34198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34198
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0022"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(72..476)
                     /locus_tag="L3M60_000961"
     CDS             complement(72..476)
                     /locus_tag="L3M60_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HBN6225654.1"
                     /translation="MKKYKVIFIVLIIGIISVTGFVVYKNYVKPWKDAEIYIDKYMVK
                     QDISKDNVKSITKEKAKKASYEGILYKVYYKDDPRYEYQYFYSDNYYALYVNKVMLQI
                     YDMENNNKLLKSNEELKSVKYPPIYLKYKPDK"
     gene            complement(668..1855)
                     /locus_tag="L3M60_000962"
     CDS             complement(668..1855)
                     /locus_tag="L3M60_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HBN6225655.1"
                     /translation="MSKIVFFSIPAYGHTNPTIEVVRELVHRGNEVLYYSFNEFRDKI
                     EGAGAKFICCDKYLPELLPGDEKKIGKDFPRLIEMIVDTTISLDEKVCRELKDFNPDC
                     IVSDSLSFWGKLFAQKLNIPYVCSTTTFAFNKHTAKLMKQSFIEIIRIIFGVRRINKK
                     IKLLQRKGYEVKNFISIVSNDNDTDTIVYTSKEFQPMVETFSSKYSFVGPSVSKLIIE
                     PKERKRKLIYISLGTINNKNVSFYKNCINAFKDSDVDVIMSVGRSTDIESLGSIPNNF
                     KVKNSVEQIAILQKTDVFVTHSGMNSVNESLYYGVPMVLFPQHSEQRMVANRVVHLGA
                     GIMLKEDKSESIKKAVFQVINDNEYKENATKLSKSFYNAGGSKKAADVILQIICNSRQ
                     VDI"
     gene            complement(1887..2210)
                     /locus_tag="L3M60_000963"
                     /pseudo
     CDS             complement(1887..2210)
                     /locus_tag="L3M60_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
     gene            complement(2248..3870)
                     /locus_tag="L3M60_000964"
     CDS             complement(2248..3870)
                     /locus_tag="L3M60_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN6225656.1"
                     /translation="MKQPYNTTIYNTALYMRLSRDDELQGESGSIQTQRMMLRQYAAE
                     HSLNVIDEYIDDGWSGTNFDRPDFQRMIDDIEDGKINCVVTKDLSRLGRNYILTGQYT
                     EIYFPSKGVRYIAVNDNVDTINGENELAPFLNILNEMHARQTSKKVKAAMRTRFANGA
                     HYGAYAPLGYVKDPDKKGHLLIDPETRWIIEKIFDLAVHGRGAASITRILVEEKVPTP
                     GWLNFQRYGTFANIYAGAPEEKAYAWTIAQVKSILKEETYIGHSVHNKQTNISFKNKK
                     KVRKPKEEWYRVENTHEAIISEDVFRQVQEQICNRRRRQKNGTTQIFSGLVKCADCGW
                     SLAYGMNSQNKNPYAHYHCSKYGQGLHQCSMHYIRYDVLYAYVLSRLQYWSVLAQQDG
                     DKLLKRLLNASDKERNTARKRQTAELKKAEKRKAEVDTLFAKMYEDWSAGRITEYNFN
                     MLSEKYQGEQRELDAKIERLHEAMEVAAQTAVDAEKWIGLMKQYVNPTELTAELLNTL
                     IEKILVHEAVKSEDGSREQEVEIFYRFIGKIE"
     gene            complement(3971..4165)
                     /locus_tag="L3M60_000965"
     CDS             complement(3971..4165)
                     /locus_tag="L3M60_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225657.1"
                     /translation="MAKSESDIFTPRTGQVIQAENGTQYFVCGNNRIKISEHFAAGGK
                     PLGDLIVDVVRHTAEKAAST"
     gene            complement(4238..5182)
                     /locus_tag="L3M60_000966"
     CDS             complement(4238..5182)
                     /locus_tag="L3M60_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBN6225658.1"
                     /translation="MAKHAILRFEKHKGNPARPLEAHHERQKEQYASNPDIDTSRSKY
                     NFHIVKPEGRYYHFIQSRIEQAGCRTRKDSTRFVDTLITASPEFFKGKSPKEIQAFFQ
                     RASDFLIGRVGRENIVSAVVHMDEKTPHLHLTFVPLTKDNRLCAKEIIGNRANLTKWQ
                     DDFHAYMVVKYPDLERGESASKTGRKHIPTRLFKQAVSLSKQARAIEAALDGINPLNA
                     GKKKEEALSMLKKWFPQMENFSGQLKKYKVTINDLLAENEKLEARAKASEKGKMKDTM
                     ERAKLKSELDNLQRLVDRIPPDILAELKRQQRHTVKER"
     gene            complement(5365..5760)
                     /locus_tag="L3M60_000967"
     CDS             complement(5365..5760)
                     /locus_tag="L3M60_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBN6225659.1"
                     /translation="MTNTIYIHQPEKAFSFTRLPNFLFEAPTFKPLSNEAKVLYAFIL
                     RRTELSRKNGWADDCGRIFLYYPICEVVTLLHCGRQKAVNTLRELQYAGLVEIQKQGC
                     GKPNRIFPKSYEAVPNTDFKKSGSGTPED"
     gene            complement(5872..6285)
                     /locus_tag="L3M60_000968"
     CDS             complement(5872..6285)
                     /locus_tag="L3M60_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBN6225660.1"
                     /translation="MRDNPYKDLPPLERRPDGSLYRMTPAQRKQAASLIRRECCCCED
                     GNCIVLDDGDTCTCPQTISFSVCCKWFRWAVLPLDGTLEAEIVRDKDLKRCAVCGGVF
                     VPKSNRAKYCPGCAARVHRRQKTESERKRRSAVDS"
     gene            6625..7422
                     /locus_tag="L3M60_000969"
     CDS             6625..7422
                     /locus_tag="L3M60_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225661.1"
                     /translation="MALPGTPGQRISDLCNGNHITQKELAEKIGVSASQLSRIVSGET
                     RTVSSDILIGVAKEFKVSTDYILGLSTVSVRKSYDISELGLSEGAVRGLVTGAVDVQI
                     LNRLLEHRNFPKLIDLIRIYFQDTAAKGITARNQLIEIATASLSDLMKEHPEHRAEAK
                     QDLQLLNAQKMGEHEAEIEKIKNVFLAILRDIKKDIDNGEQPGEAVTAAMFQAMRDAL
                     AEQKQNPLSIDDVAAMVAGQIGQLTPMDEETADLFKQLAKKMMKGIE"
     gene            7447..8217
                     /locus_tag="L3M60_000970"
     CDS             7447..8217
                     /locus_tag="L3M60_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HBN6225662.1"
                     /translation="MPLVKHDYPVLEYDTASKAVFQPGNGKKCFPAKAVFAFLGDEVE
                     NYAHTHDGIQIDEFESATRRYPIYECLYNQEKICLCPAPVGSAAAVQVLEYLIAGGVT
                     KIISVGSCGVLEDIPENRFLIPVSALRDEGTSYHYLPPSREVEISKAGINAIESALSQ
                     KNIPYWEVKTWTTDGFYRETVEMVQYRKEEGCQVVEMECSALAACAKFRKVTWAMLLF
                     SADTLADPHKYQEREWGKTSISIALELALDAVLSVVEE"
     gene            8242..9018
                     /locus_tag="L3M60_000971"
     CDS             8242..9018
                     /locus_tag="L3M60_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(9) family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HBN6225663.1"
                     /translation="MNINEFPQQVNQVISIAETILQGQILGIYLYGSATMNGLRPDSD
                     IDILIITKQELSNSIRADLTKQLLKISGSVGCIEKRPLEVTIINQSDIVPLQFPPKCQ
                     YMYGEWLRGEMEAGEYPQACNDPDIMILLWQARKNSITLKGAESKELIPAIPFHEIKK
                     AIRFSLPGLISSFKGDERNVLLTLSRMWFTLVTEEITTKDVAAKWVILKLPERFPPLL
                     TTAKEAYLGNLSDEWETVEKEAMALVEYMKKQIEELLRTE"
     gene            9218..9763
                     /locus_tag="L3M60_000972"
     CDS             9218..9763
                     /locus_tag="L3M60_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6225664.1"
                     /translation="MNQGRIIVITGSPGTGKTTTASIVAKESNMDKSVHMHTDDFFHY
                     LSKGAIPPHLPESNEQNLVVIEAFLEAAKRYARGGYDVIVDGIVGPWFLEPWRALVRE
                     DYEVHYIVLRASKEETMKRAVERSKLDRKTNVELVETMWEQFCNLGIYESNVIETTTY
                     SIQEAVFAVKEKISSGAALLS"
     gene            complement(10502..10621)
                     /locus_tag="L3M60_000973"
                     /pseudo
     CDS             complement(10502..10621)
                     /locus_tag="L3M60_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(11059..13008)
                     /locus_tag="L3M60_000974"
     CDS             complement(11059..13008)
                     /locus_tag="L3M60_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN6225665.1"
                     /translation="MKRKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMVHGGHGWLINQFLSPYFNKRNDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGVEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVIPVLKPKKVL
                     IAGGGPGGLQTAITAAKRGHKVILCEKTNELGGILKGEQALPFKYEMYELGNTFGKIA
                     EDLGVEVRLNTRVTKEYVENENVDALIIAVGSEPLVPSIEGLDGENVVIVNDYYLEKE
                     KVTDEVVVLGGGLAGCEAAIHLAQEGKTVHLVEMRAELAPDANIRNRPILLQEIEKQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDALLDCAPYVA
                     QIGDCVRASTITNAVYQGHHVALDI"
     gene            complement(12980..14278)
                     /locus_tag="L3M60_000975"
     CDS             complement(12980..14278)
                     /locus_tag="L3M60_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBN6225666.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSIAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLVFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWIPPEKKGKVRVIINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDAAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLAGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYVGPAVMKNVYATDGNYQRAFIIAIVI
                     ALIGFILTFLYKYINKIQNLKIENLMKGDIKDEKKISKSM"
     gene            14529..15086
                     /locus_tag="L3M60_000976"
     CDS             14529..15086
                     /locus_tag="L3M60_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6225667.1"
                     /translation="MRTLKYAILGLLNRNPMTGYDIVKEFNFQLAEFWNAKHSQIYPE
                     LKKLVNEKLIVYDIKISGDVLEKKLYTITEKGKDEFLKWLEKDEPMERTPKDIFRLRM
                     YFSNNLDIDTRLILLEHQLLQHQERIAFLYSQMERYSEVPPLNSNDFGDYIVLDGAII
                     REQGAIKWLTNCINHHKNLDNTTLK"
     gene            15510..16259
                     /locus_tag="L3M60_000977"
     CDS             15510..16259
                     /locus_tag="L3M60_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440038.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBN6225668.1"
                     /translation="MILYFSGTGNSRYVARKIAQELNDELISLNQLIKEEKTDELISA
                     DKPFIFVCPTYAWRLPIVVTDFIKKTKFLGSNRVYFVMTCGGDTAKSINYIQKLCKYK
                     EWQLKGMAEIKMPENYIALFSTTDKETSKQMIKEADKQIYRIISDIRNENEFETITPS
                     GLGGAIKSGIINTVFYKTIISAKGFHYTDKCIGCGKCVELCPLNNINLKNKKPVWKNN
                     CTHCMACICGCPTEAIEYKNKTQNRERYYLE"
     gene            complement(16419..16784)
                     /locus_tag="L3M60_000978"
     CDS             complement(16419..16784)
                     /locus_tag="L3M60_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN6225669.1"
                     /translation="MEKECQQRMQEITSKFKECRKAISAMGDETRQLILITLLESDFN
                     GIRVGEITEKTHLSRPAVSHHLKILKEAEIVNVRREGTKNYYYLDSKKSQWKSLTELI
                     NLIFVGIQHISENDIRKGD"
     gene            complement(16800..17576)
                     /locus_tag="L3M60_000979"
     CDS             complement(16800..17576)
                     /locus_tag="L3M60_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6225670.1"
                     /translation="MKYQHIIKVDKELCIGCGLCKNDCPVNNIIIENKKSVIKKQDCL
                     MCGHCAAICPQKAITLTGFDEPPIELTNKPNLDSDELLMAIKSRRSIRKFKDKEVSLE
                     IVKQIIEAGRYTPSAKNSQDVSYIVLDNKKSIYENEAVKFFRKIKPIANIAIKYSKEV
                     EIDDKFFFKNAPIAIMIITKDKISGSLAASNMSLMAESYGLGVLFSGYFSDVANNSPK
                     LKKLLGLKRSNHVLTTLVIGYPDVKYRRTAQKEAANVRYL"
     gene            complement(17995..19422)
                     /gene="ascB"
                     /locus_tag="L3M60_000980"
     CDS             complement(17995..19422)
                     /gene="ascB"
                     /locus_tag="L3M60_000980"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBN6225671.1"
                     /translation="MSFKNDFLWGGATAANQCEGGYNEDERGLANVDVCPTGKDRTAV
                     ITGKLKMFDFDDKHYYPAKTGIDMYHNYKEDIKLFAEMGFKVYRMSIAWSRIFPRGDE
                     ETPNEKGLQFYENIFKECKKYKIEPLVTITHFDCPMHLVKKYGAWRNRKMIGFYEKLC
                     NVIFRRYKGLVKYWLTFNEINMILHAPFMGAGICFEEGEDVEKIKYQAAHHELVASAI
                     ATKIAHEIDSNNLVGCMFAAGSVYPYSCNPNDVWEATKLDRENYFFVDVQSKGKYPNY
                     ALKYMEQKGIAPEMESGDIELLNKYTVDFISFSYYNSRCVRTDENAGDMAEGNIFTSA
                     KNPYLSYSQWGWPIDPLGLRITLNHVYDRYEKPLFIVENGLGAKDIADENGYVEDDYR
                     IDYLREHIKAMHDAVYIDGVDLLGYTTWAPIDLVSAGTGEMEKRYGFIYVDRDNSGNG
                     SLRRTKKKSFEWYKKVIASNGEDLY"
     gene            complement(19483..21411)
                     /locus_tag="L3M60_000981"
     CDS             complement(19483..21411)
                     /locus_tag="L3M60_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6225672.1"
                     /translation="MGKYKQLAQEIVKNVGGKENISGLVHCITRLRFTLKDESIANDN
                     VLKNMEGIVTVMKSGGQYQVVIGNHVEAVYKDVVEIIGLDNSSANSETKKSGSILDKG
                     IDIISGIFQPVLGIMAACGMLKGFNALFVAMGLYSDVSGGYMVINAAGDALFTFLPLF
                     LGYTSAKKFGLKPMLGLALGAAVCYPAIQGSSISAGADVMYTLFKGTMFASPVYIDFF
                     GLPIVSIDYTGTVVPIIFIVYFASRCEKLFNKFIPDLVKFFFVPMLTLLITVPVALIV
                     LGPIATFGSTVISQVVISIRDFSPLLAGAIVGFTWQILVIFGMHWGFIPVYINNVMTL
                     GYDNVMMPFFACTFASSAVVLAIFFKTKDKKLKEMAIPNFISGIFGVTEPAIYGILLP
                     LKKPFIISCIASGIGGAFYGFFNLRKFITGGMGIFELPAMIEPDGGMGNLIVALSGIA
                     ISMVVAFVLTMILYKDEKVEESKKVRNKGKEEIKEVKSTKLEREIVASPIKGEVLKLS
                     EIEDAAFASGVLGQGVAIIPSDGKVVAPVDGVVTTLFPSLHAIGILSDAGVEVLIHIG
                     LNTIQLEGRCFEACIKQGDRITKGQTILNFDIDAIKELGYSVVTPIVITNSSQFLDVV
                     ETGRKNIELEDNLITVLF"
     gene            complement(21556..22398)
                     /locus_tag="L3M60_000982"
     CDS             complement(21556..22398)
                     /locus_tag="L3M60_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6225673.1"
                     /translation="MIINKILNNNVVITLDDNDEEIIVMGKGIGYQKSKGNLIDKTKV
                     NKVFRISNKEVSNKLQELLNNIPIEHMKLSSEIIEYAQIKLNKKLNESIYISLSDHTY
                     SAIQRMKEGINVKNAILWEIKRFYKEEFEIGMKALDIIENKTGVKLPEDEAGFIAFHI
                     VNAQLSEGHTFASDITKLIQEVLSIVRYHFRIEFHEESVFYYRFIMHLKFFAQRLLLN
                     NAHEGGTDKELLSIIKSKYNKEFECVVKIKDFIKKQYNYILTDDELIYLTIHLAKVVK
                     DSNI"
     gene            complement(22570..22716)
                     /locus_tag="L3M60_000983"
                     /pseudo
     CDS             complement(22570..22716)
                     /locus_tag="L3M60_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893832.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(22816..23265)
                     /locus_tag="L3M60_000984"
     CDS             complement(22816..23265)
                     /locus_tag="L3M60_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225674.1"
                     /translation="MEIREILSEESIILDLKSTTKEGIIQELVDKLYDTGSIDLKSKF
                     IKALKRREKIGSTSIGYQMAMPHGKTKYVKKASVVIGKSKKGIDWPSIDGLPVKVIIM
                     FAMPQSGDNEDMTLISKIAYILGGKDISERLLNANTKKEILDIFYEV"
     gene            complement(23255..25162)
                     /locus_tag="L3M60_000985"
     CDS             complement(23255..25162)
                     /locus_tag="L3M60_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6225675.1"
                     /translation="MLVSRQIEFLKLILNENKYRPIKYFKDKLNVSDKTLQKDLKIIE
                     KYLETFNIKIDIKRGYGILIESLAKKNIDLINSLNIQSKENKSLSVEQRRMEILKYLL
                     LNSNNITSINQLADKYYVSKTSIVNDFKYIEAWIKEYNLKLNKTLEGTKIIGKEVDIR
                     KSIAKMMDDLLDENYEQHDISKLTRLDSATFSALINLFDIDSIIFVETIITELEYNLG
                     YTISQPYYINLITHILICLKRIEEGNQIESQEEREVSVDNLDELVYQNVTALIGKIEN
                     RYGVKITKDEIMYIYIFLVSSGFSSESNNSYNDESYRLSESEKFSSIMIENMSQFLNI
                     NLKNDDLLQKSLASHVRPMLNRLRYDIQIKNPLLGEIQERFSDVLGLCMMTINIMTEY
                     DNLKNISIDEVSYLATYFQAAIERNMSSKRVIVVCHTGYGTSQLLAARLKREFPEWTI
                     VDIVPMHQLSNRNLDDVDFVISTVNLDLKDKLHIVVSVLLMESDISNIKNALLSKPKK
                     SMNLDTNLLGMYLEDNIYFNFDVMQMESLIGVDLGSKYKSISLGKDLNIYIHNQSKLN
                     KGIMNVNNINRTIDIYLETSNQSFLKNILMEISNLYRQKSYINYIIDCKSKEDIKILF
                     KNNLLEKTNGN"
     gene            complement(25429..27324)
                     /locus_tag="L3M60_000986"
     CDS             complement(25429..27324)
                     /locus_tag="L3M60_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225676.1"
                     /translation="MIGQILRSDCIVLDKQLQTKKDVIDYMANHLYHGNYINDKEKFI
                     VDINKREKESSTNMGNLIAIPHAKSNVVNQPVMMYLRTDKEITDNDGQKLRSFFMIAV
                     PESGSDEHLKIISKLATYLMDENLLKHLLSAETPYEILSIFNNKAVESGDSSDNQNKG
                     LIVGITGCPTGIAHTFMAKKALEEAGQELGYEVKIETHGAIGVENKLTKEDIKRAEYV
                     IIACDKEVQMSRFIGKKVLQKKVADAKNVSLAKNLIINAENGMAKEIKPGGRTLEDSK
                     EILQGSEKKGVYKHLMSGVSEMIPFVVIGGIGIAIAFMFGIFASDPTHETYNPIAGFF
                     GQLGGDSAFKLFIPILAGFIAKSIAGRQGFAPAMIAGFMAVIGGSGFIGGMVAGFSTG
                     YIALGVMKITENLPDVLKGVNAVLICPLLIAFTSGAIMFFVVNTPASWLNEALQSWLQ
                     SMSGTNKVLLGAILAGMMASDMGGPINKTASAFGLAMFSSNIFEPSAALMVGGMVPPL
                     AISLAMLIFKNKFTKEERKSSLPTAIMGASFITEGAIPFAAADPLRMIPANIIGSCIG
                     GAICMSLDITLMAPHGGIFVIPFACSSPVAYIACILIGTVITALIIGVTKPTIRSENS
                     EVEVLGA"
     gene            complement(27360..28238)
                     /locus_tag="L3M60_000987"
     CDS             complement(27360..28238)
                     /locus_tag="L3M60_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HBN6225677.1"
                     /translation="MLINMKEMLKVAQENQFAVPAFNIGSGQILKAVVQSANEKNAPV
                     ILAIHPNELSFLGDSFVASCIEEANKSKVPMVIHLDHGENKEQILRAIRCGFTSVMID
                     GSHLPYEENVAISREVVEIAKGLNVSVEGELGTIGTTGTSSEGGTDEIIYTDSKLAKD
                     FIEKTGVDTLAIAIGTAHGIYPKGFKPELKLDLLKEIREVVDIPLVLHGGSSNPDEEI
                     AQAVKLGVCKINISSDVKSAYYKKCREVLEKNSSLYEPDTIYPPCIDAAREVIEFKMN
                     LFNTIDKLKCFYKNKN"
     gene            complement(28790..30484)
                     /locus_tag="L3M60_000988"
     CDS             complement(28790..30484)
                     /locus_tag="L3M60_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBN6225678.1"
                     /translation="MIKKIKRSKFTPKNVISIILYSILFWILIGYIIIPIGNTFTQAF
                     QGKGGYSFEVFKEYFSNTNNLKVVSNTIILGFGSVLVCGIMGISLALYMTFVCDKHKK
                     IIHILLLSPMMIPGVIMVIACIQLYGESGIVTKSIEILLSFIKKPYDFSGLKGVLFVV
                     AYTQYVYFYLNVYVALKYVDYSTVEAAKGMGASKIRVFFDVIWPVITPAIITSTIITF
                     ASGISSFSAPNLIGGGFKVLSTQIVRSKANNHMDIASVQVIILLLMGISFMILLQYYG
                     KKYGVVASLKSQGYKVHNRKKDLFTFVCKVIIEIQIVMIMLPIVTIVYLSFVSTHSIM
                     TTIFPRDFTLENYQMIFQKSRVLQPILNSLKMSLMAVVIGLIITIPVSYLVIKKDTTY
                     NRLAKFIIMLPWSMPASAIAVNLINTFNQKSIFAFNESLIGGFYIVPIAYTIYALPLL
                     FSSNEVAMKSINLGLEESSRSLGAGRIKTFYNVIVPNMMPGIISGAILVFIRTIGEYT
                     MSALLYGVYNRPISISIVTNMQEFKIGVSMAYGVLVIGICYIALGIIFKLDRKRFT"
     gene            complement(30486..31478)
                     /locus_tag="L3M60_000989"
     CDS             complement(30486..31478)
                     /locus_tag="L3M60_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225679.1"
                     /translation="MKNIELKEINHYYGKEKVLYDINISIKEGEFFTLLGPSGCGKTT
                     ILRIIGGFIKPSSGSIYVGDKNITNLEPENRNMGTVFQNYALFPNMTVEENVSYGLKI
                     KKLSKKIIKEKCDNYLELAGMKDFRKKKIDELSGGQQQRVAVARSLATEPTMLLLDEP
                     MSNLDIALRIKMREEIREIQQKIGITTLFITHDQQEALAISDRIAVMDKGKVLQIGVP
                     MEVYKKPLNDFVANFVGTSNCIEKEDYVNFNIEKETKPYIYKRPEEMVLLQNTNQSGF
                     IKVKVESKNFLGAILEYTVSNNGKKYQVTELNRLNNSNKLNVGDMAYLGVLQGN"
     gene            complement(31497..32354)
                     /locus_tag="L3M60_000990"
     CDS             complement(31497..32354)
                     /locus_tag="L3M60_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225680.1"
                     /translation="MGLKISDYKLLCNKGNGDFNEDIVGLSPYGAWVLDGATGLNNKN
                     LVSTESDAKWYVSWWNSYLHENMEREDSLKVIIKEGIEEITKEYYKLTYDKEVEAIDI
                     PSCSCSILKFYKDKIEYLLLGDCTLLVNHNESVKILKDETLCRLDKKIFDFMQEAKDV
                     TNLTLKERKAEVMPIIIENRLKKNTVGGYWALEFSKEAVDNCISGFIDIEYKMKIMMA
                     SDGFSCGYDRYKIFDKNEMMKIAEIKGIDYIYRKVRELEKDDEMAIEFPRFKIYDDSS
                     CVYLEIYKY"
     gene            complement(32632..33675)
                     /locus_tag="L3M60_000991"
     CDS             complement(32632..33675)
                     /locus_tag="L3M60_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBN6225681.1"
                     /translation="MKRKIAVVLLTVAMLLSLVACSNDNTSKSDKLEDTLVVYTTHGE
                     DMLGAISEEFTKETGVNVEFINLKGELADRVRSEKDNPQADIMFGGDTSTYMQLKEEG
                     LYEKTKPSWNNELDKSYKDSDGYWYGTIKTPVMIFYNTKLLSADKVPKDWSDLTKEEY
                     KDLIVTRDSLSSSMRSTISNLINYYSNTKSEEEAWKYLEDLNKNIKNYYNSGSVMYSA
                     IGKGEAAISVAVLSDIIENKEKNNMPLEVVDAESGSVVITDCVAALKNAPHPNAAAKF
                     VDFVGSAKVQAMVANDFNRIPTLDSALKDSPKWMSKEYKAMDVDWSVIGKNQSNWIEK
                     WETDIIDNNKTIK"
BASE COUNT        10509 a         6563 c         4798 g        12328 t
ORIGIN      
        1 taattttatt taaacactct agtgatattt ttaaataaat tttaataaaa atccttctat
       61 tttgtcataa atcatttatc tggcttatat ttaagatata ttggtggata ctttacactt
      121 tttaattcct cattactttt taaaagttta ttattatttt ccatatcata aatttgtaac
      181 attactttat ttacatatag agcataataa ttatcactat aaaaatattg atactcatat
      241 cgagggtcat ctttgtaata cactttgtat agtattcctt cataacttgc cttcttggct
      301 ttttcttttg taatactttt tacattatct tttgaaatat cttgctttac catatactta
      361 tcaatatata tttcagcatc tttccatggt ttaacatagt ttttatatac aacaaaccct
      421 gttacactta taatacctat aattaatact ataaaaataa ctttatattt tttcatatta
      481 atcctccttc atttattgtt taaaacttaa tataatgcat taaaaaaagg atgttatctt
      541 ttttattaaa atataacatc cctcatattt agcatcaata tatatgcccc aactagtagg
      601 ttaaaaggcc ccactgtcag tactttgatt aaattgcatg tatgtatata tatttttaaa
      661 aaatctatta tatatcaact tgtctactat tacatataat ctgtaatatt acatctgctg
      721 ctttttttga gcctcctgca ttataaaagc tttttgatag tttagttgca ttttctttat
      781 attcattgtc atttataact tgaaatactg cttttttaat actttctgac ttatcctctt
      841 ttaacataat tcctgctccc aaatgaacaa ctctattagc taccattctc tgctccgaat
      901 gttgaggaaa taataccatg ggtactccat aatataaact ttcattaaca ctattcatcc
      961 cagaatgagt aacaaacaca tcagtttttt gaagtatagc aatctgttca acactatttt
     1021 taaccttaaa attatttggt atacttccca aactttcaat atcagtactt ctaccaacag
     1081 acataatcac atcaacatca gaatctttaa atgcatttat acagttttta taaaagctaa
     1141 catttttatt attaattgta ccaagcgaaa tatatattag ctttcttttt ctctctttag
     1201 gttcaataat caactttgat actgatggac ctacaaatga atacttacta gagaaggttt
     1261 ctaccattgg ttgaaattct tttgatgtat atacaatagt atccgtatca ttatcattag
     1321 aaactataga aataaaattt tttacttcat acccttttct ttgtaaaagc tttatttttt
     1381 tattaatacg tcttacacca aaaattatac gtataatttc tataaagctt tgtttcatca
     1441 actttgctgt atgtttatta aatgcaaaag ttgttgtaga gcatacatat ggaatattaa
     1501 gtttttgtgc aaataacttt ccccaaaaac ttaatgaatc tgatacaatg caatctggat
     1561 taaaatcttt aagttctcta caaacttttt catctaaaga aatagtagta tctactatca
     1621 tctcaattaa tctgggaaaa tccttaccaa tttttttctc atctccagga agtagttctg
     1681 gaaggtattt atcacaacaa ataaattttg ctccagcacc ttcaatttta tctctaaatt
     1741 cattaaaaga ataatacaat acttcatttc ctctatgcac taactcacga actacttcaa
     1801 tagtaggatt agtatgacca tatgcaggaa tgctaaaaaa tactatttta ctcatatttc
     1861 atacctcctt ttataatttt ttaaaatcat tcttctttag gtactactcc cacatgacct
     1921 ataaaattta tcaaatcacg ttgatgaaca attcctaatc ctctttcctc atctccaagt
     1981 accaataatt tatctcctgc atttatatta aatacttctc ttgcttcttt tggaatgaca
     2041 atttgaccac gttcaccaac tactactgct ccaaagaaaa actttccttt aggaggtact
     2101 ataatacctg ttttttcttc cgaatgatta actaagtcat ctagtttgac attgtacaat
     2161 tttgcaagtt taatgcaact acctatatca ggaatagatt ctcccgtttc ccttagttaa
     2221 agatattgtt gggtatctca agatgtgtca ttcgattttg ccgataaagc ggtagaagat
     2281 ttccacttcc tgttcccggc ttccgtcctc acttttgacc gcttcatgga caaggatttt
     2341 ttcaatcagc gtattcagaa gttcagccgt caattctgtg ggattgacat actgtttcat
     2401 cagacctatc cacttttcag cgtcaaccgc tgtctgggcg gcgacctcca tcgcttcgtg
     2461 aagccgttca atttttgcgt ccaattctcg ctgttcgccc tgatactttt cggacagcat
     2521 attgaaattg tattctgtaa tgcgtccggc agaccagtcc tcatacattt ttgcaaacag
     2581 ggtgtctact tctgctttgc gcttttccgc ctttttcagt tcggctgtct gccgcttcct
     2641 tgcagtattg cgttccttgt cgctggcgtt aagtagccgt ttcagaagtt tgtccccatc
     2701 ctgctgtgcc agcacagacc agtattgcag acgggaaagg acataggcgt aaagcacatc
     2761 atagcggata tagtgcatgg aacactggtg caatccttgc ccgtacttgc tacaatggta
     2821 gtgggcatag ggatttttgt tctggctgtt cataccatag gccagcgacc agccgcagtc
     2881 cgcacatttt accagcccgg aaaatatctg tgttgtgccg ttcttctgcc gtctgcgcct
     2941 gttgcaaatc tgctcctgta cttgacggaa cacatcttcg gaaataatcg cttcgtgggt
     3001 gttctccaca cgataccatt cctcttttgg cttgcgtact ttcttcttgt ttttgaatga
     3061 aatattggtc tgcttattgt ggacgctgtg tccgatatag gtttcctctt tcagaatact
     3121 tttcacctgc gctatcgtcc acgcataggc tttttcctcc ggcgctccgg catagatatt
     3181 tgcgaaagtg ccgtatctct ggaaattcag ccagccggga gtaggtactt tttcttcgac
     3241 caaaatccgt gtaatgctgg cggctccccg gccatgaacg gcaaggtcaa aaatcttttc
     3301 gataatccac cttgtttccg ggtcaatcag aagatggcct ttcttatctg ggtctttgac
     3361 atagccaagc ggagcatagg ctccatagtg tgcgccattt gcgaaccgtg tccgcatggc
     3421 cgcctttacc tttttgctgg tctggcgggc gtgcatttca ttcagaatgt tgaggaatgg
     3481 ggcaagctca ttctctccgt tgatggtgtc cacattgtca ttgacagcga tatagcggac
     3541 tcctttgctg ggaaagtaga tttccgtgta ttggccggtc agaatgtagt ttctgcctaa
     3601 gcgggataaa tccttcgtaa caacgcagtt gattttcccg tcctcaatgt catcaatcat
     3661 tctctgaaaa tccggcctgt caaagttcgt tccagaccat ccatcgtcga tatattcatc
     3721 tatgacattc aggctatgct cggcggcata ttgccggagc atcatgcgtt gtgtctgaat
     3781 actcccgctt tctccttgca gttcatcgtc ccggctcaat ctcatataaa gcgccgtgtt
     3841 gtaaatcgta gtattgtaag gttgtttcac cgttaaaaat cctccttcta aagaaacaac
     3901 ccacgcttac aatacttttg ctctatggca attatatcat aagcgtgggc gtgttatcaa
     3961 tgatgggcta tcaggttgaa gcggcttttt ctgcggtatg ccgcaccaca tctacaatca
     4021 gatcaccgag gggcttcccg cctgcggcga agtgttcgga gatttttata cggttgttcc
     4081 cacatacaaa atactgcgtg ccgttctctg cttgtatgac ctgccctgtc cggggtgtaa
     4141 aaatatcgct ttcgcttttt gccatccagt gtcctccata ttcagttttc aaggtacaat
     4201 gccgcacaaa ggcggcgaaa atttctgctt atatctatca cctttccttt accgtgtgcc
     4261 gctgctgacg tttcagttcc gccagtatat ccggcgggat acggtcaacc agccgctgta
     4321 aattgtccag ttcgcttttc agctttgccc gttccatcgt atctttcatc tttccttttt
     4381 cgctggcctt tgcccttgct tccaacttct cattctccgc caacaggtca ttgattgtga
     4441 ccttgtactt tttcaactgc ccggagaagt tctccatctg cgggaaccac ttttttagca
     4501 tggagagggc ttcctctttt ttctttccgg cgttcagcgg gttaatgccg tcaagggcgg
     4561 cttcaatggc tctggcctgt ttggagaggg aaaccgcctg tttgaaaagc cgggtgggga
     4621 tatgcttccg gcctgtcttg ctggcactct ccccacgctc caagtcggga tatttcacca
     4681 ccatataggc gtgaaaatcg tcctgccact tcgtcaggtt tgcccggttg ccaataatct
     4741 ctttagcgca caggcggttg tcctttgtca gcggaacaaa ggtcaaatgc aggtggggcg
     4801 ttttctcgtc catgtgtacc accgccgaca cgatattttc ccgtcctacc cggccaatga
     4861 ggaagtccga cgccctttgg aagaacgcct gtatctcctt tggggatttc cccttaaaaa
     4921 actccgggct ggcggttatc agcgtatcga caaaccgtgt gctgtccttg cgggttcggc
     4981 acccggcctg ctcaatccgg ctctgaatga agtggtaata gcgtccctcc ggcttgacga
     5041 tatggaagtt gtatttgctc cggcttgtgt caatgtcggg attgctggca tactgttctt
     5101 tctgtctttc gtgatgggct tccagcggcc ttgccgggtt gcccttgtgc ttctcaaacc
     5161 gtaaaattgc gtgttttgcc attctgctcc tttccccatt cctttcctat ccggggtttt
     5221 ccacagggaa aatcccgcag aaaatagctc ggataggatt ggaatggata gaatataaat
     5281 caatcttttt atctttgtat ttctatatac cgtccggttt tcgtacgtct caggagtgat
     5341 ttccgtactt catgggtacg gttttcagtc ctccggcgta ccagaaccgg atttcttgaa
     5401 gtcggtgttt ggaaccgctt cataggattt tgggaaaatg cggttgggtt ttccacagcc
     5461 ctgcttctgg atctccacca gtccggcgta ttgcagttcc cgcagggtat tcaccgcttt
     5521 ctgccgccca caatggagca gtgtgaccac ttcacagata gggtaataca ggaaaatccg
     5581 tccgcagtca tccgcccacc cattcttgcg ggataactct gtccggcgca ggataaaggc
     5641 gtacagaacc tttgcctcgt tagacagggg cttgaatgtg ggggcttcaa agaggaaatt
     5701 cgggagccgg gtgaagctga acgccttttc cggctgatgg atatagatgg tatttgtcat
     5761 agttcgtttt gtggacggtt agaagcccgt tttccggggc gggcgatact ttataccacc
     5821 tgccccggtt tggggcttgc aaagcctgat aaatcaaggc ttttttcgct cctaactgtc
     5881 cacagcagac ctccttttcc gttcactttc tgttttctgc cgcctgtgaa ctctggcggc
     5941 acagccgggg cagtattttg cccggttgga tttggggacg aacacgccac cgcagaccgc
     6001 acagcgtttc aagtccttat cccggacaat ttccgcttcc agcgtcccgt ccagcggcaa
     6061 gaccgcccag cggaaccact tacagcagac cgagaaagaa atcgtctgcg ggcaggtgca
     6121 ggtgtcccca tcatcaagga caatgcagtt gccgtcctca cagcaacaac actcccggcg
     6181 tatcaggctg gccgcctgtt ttctctgcgc cggtgtcatg cggtaaaggg aaccgtccgg
     6241 cctgcgttcc agtggcggca agtctttata ggggttatct ctcatgcgtt ccctccattt
     6301 tgctttccgt tgccgttctc cccgaaaagg tttcctgccc cattcctgcg gcgtgttccg
     6361 gcgttggcac gctccccgca gcttcgggac attttgtctc gaagtccggt tccttgcggt
     6421 gcttccccag ccggggtatt ccttttcgga cggtcattct gttttcaagg tgctgtccat
     6481 cgatgaacta ccctaagtct acacgaaaaa aatgcaatcc ccggaatttt gcgagaattg
     6541 cattttgatg aagtttacac tttggaaatt gcattttttg caatcattct gtataatgga
     6601 agtatgcgga ggaggtgcgt atctatggct ttacccggaa cacccggaca gcggatttcc
     6661 gatttatgca acgggaacca catcacacaa aaggaacttg cggagaagat aggggtttcc
     6721 gcttcccagt tgagccgcat tgtcagcggc gaaacgagaa cggtcagcag tgatattctc
     6781 ataggcgtgg caaaggaatt taaggtatcg acagactaca tactgggctt atccaccgtg
     6841 agcgtccgta aaagctacga tatttctgaa ttaggcttgt ccgagggagc cgtgaggggg
     6901 ctcgtgacgg gtgctgttga tgtgcaaatc ctcaaccgcc tgttggaaca caggaatttt
     6961 cctaagctga tagatttgat acggatttat tttcaggaca cagcggcaaa aggcataaca
     7021 gcaagaaacc agcttatcga gatagcaacg gcttccctgt ccgacctgat gaaagaacac
     7081 ccggaacacc gggcggaagc aaagcaggat ttacagcttt tgaacgccca gaagatgggg
     7141 gaacatgagg cggagattga gaaaatcaaa aatgtgttcc ttgctatcct gcgggatatt
     7201 aagaaagaca ttgacaacgg ggaacagccg ggagaagctg tgaccgccgc gatgttccaa
     7261 gccatgcggg acgcattggc ggaacagaag caaaacccgc tttccattga cgatgtagcg
     7321 gcgatggttg ccggacagat cggacagctt acgccgatgg atgaagaaac tgccgacctg
     7381 ttcaagcagt tggcaaaaaa gatgatgaaa ggaatagaat aatagcctgt tattcgagat
     7441 aataatatgc cattagtaaa gcatgactac cctgttttag agtacgatac cgcatcaaaa
     7501 gctgtttttc agccgggaaa tgggaaaaaa tgttttcctg caaaagcagt gtttgctttt
     7561 ttaggagacg aggttgaaaa ttatgcacat acccatgatg gaatacagat agatgaattt
     7621 gaaagtgcaa caagacgata tcctatttat gaatgtcttt ataatcagga gaaaatatgt
     7681 ctatgtccgg ctcctgtggg aagtgctgct gccgtccaag ttttagaata tttaattgca
     7741 gggggtgtaa caaagattat ttccgtcggc tcctgcggcg tattggaaga catcccggaa
     7801 aatagatttt tgatacctgt ttctgcattg agagatgagg gaacatctta ccattatctt
     7861 cctccctccc gagaagtaga gatttcaaag gctggtataa atgcgattga atctgcttta
     7921 agccaaaaga atataccata ttgggaagtt aaaacatgga caacagacgg tttttatcga
     7981 gaaacagtag aaatggttca gtatcggaaa gaagagggat gtcaagtagt agaaatggaa
     8041 tgttccgcat tggcggcgtg tgcaaaattt agaaaagtta catgggctat gctgcttttt
     8101 tcagccgata ctcttgcaga ccctcataaa taccaagaaa gagaatgggg aaaaacaagt
     8161 atatccatag ccttagaatt agccttagac gctgttttat cagttgttga ggagtaaaaa
     8221 taaatacata taggagttac aatgaatata aatgaatttc cacaacaagt aaatcaagtt
     8281 atttcaatag cagaaaccat attacaaggt caaatattgg ggatatattt atatggttca
     8341 gcaacaatga atgggctgcg tccagatagt gatatagata tactgataat tactaaacaa
     8401 gaattgagta attcaatcag agcagatcta acaaagcaat tattgaaaat ttctggctcc
     8461 gtaggctgta ttgaaaaaag gcctttagag gtaactatta tcaatcaatc tgatattgtt
     8521 ccgttgcaat ttccgccaaa atgtcagtat atgtatggtg aatggctaag gggagagatg
     8581 gaagcaggag aatatccgca agcctgcaat gacccagata taatgatttt attatggcaa
     8641 gcaagaaaaa atagcataac tttgaagggg gcggaaagca aagagcttat tcccgctatc
     8701 ccatttcacg aaattaaaaa agcaattcgg ttttctttac ctggtttgat ttccagcttt
     8761 aagggtgatg aaagaaatgt gttattaacc ttatcacgaa tgtggtttac tttagtaaca
     8821 gaagaaatca cgacaaaaga tgttgccgca aaatgggtaa ttttaaaatt gccggagaga
     8881 ttcccccccc tgctaacaac ggcaaaggaa gcttatttgg gaaatttgtc tgatgaatgg
     8941 gagacagtag aaaaggaagc gatggcactt gtagaatata tgaaaaaaca aattgaggaa
     9001 ttacttagaa cagagtagca aagttagcgg ggccaatcaa cggtcaagat gaacggcgca
     9061 taaatgcgcc gccgttgaca gtcccgcccg tctttgctga tgggcaatca aggcgggaaa
     9121 gccctaaaat ggcttcccgc ccatttcaat cttgagagaa gaaacgctcc aaaaccagaa
     9181 agagagcggt aaatctgaat tgcgagggag aatgtagatg aatcaaggaa gaattattgt
     9241 gattacaggt tcgccgggaa caggaaagac aacaactgcg tcgattgtcg caaaagaatc
     9301 gaacatggat aaatctgtgc atatgcacac agacgacttt tttcattatt taagcaaagg
     9361 agcaataccg ccgcatttac cagagtccaa cgaacaaaat ttagttgtca ttgaagcctt
     9421 tttagaagct gcaaagcgct atgcccgcgg cggatatgat gtaattgtag atggaattgt
     9481 cgggccgtgg tttttagaac catggagagc ccttgttcga gaagattatg aagtacacta
     9541 tattgtttta agagccagta aagaagaaac tatgaaacga gctgtggaac gctcaaaatt
     9601 agacagaaaa acaaatgttg aattagtaga aacaatgtgg gagcaatttt gtaatttggg
     9661 gatatatgaa tcgaatgtta tagaaacgac aacctattct atccaagagg ctgtttttgc
     9721 agtaaaagaa aaaatttcaa gtggagccgc attgctatct taaacgattt ctaaaaatcg
     9781 aaatttcatt tatcagagaa atagcaaagg cagccaagcc agtcaacggt caaaatgaac
     9841 ggcgcatttc atgcgccgcc gttgacagtc ccgcccgtct ttgcagatgg gcaatcaagg
     9901 agggaaagcc ctaaaatggc ttcccgccca tttcattatg agaaaagaaa cgctccaaaa
     9961 tcagaacgag agcggccaag cactttgagg gattggccgc cttgtccacc cattcagcgg
    10021 gacggcgggt ggcggtcaag gccgggtgta aacccgttca tttcagcctt gacggttgcc
    10081 cgctgtcctg ttacttttcc gggagtgtag ccacaacttc gggacacttt gtccacaagt
    10141 cggagcgtag gacgagggac gggggctttc atatacgccc agtctgctgt tgaaaccaga
    10201 cctgtgctta cggctctacg ccacgcaagc acagccctgt tttcctgccg cttcaaatgc
    10261 ccttgccctc cccggcggca acggcatttt cacggcaacg ccgacagagc gtataacaca
    10321 ctacactttg cttcgcaaag tcgtgtgcca aatgggggcg ttgccccctt tggaaacccc
    10381 cacaagaaaa ggcggtacgc tacccctcca cggggcgcat accgcctttt cttgttatcc
    10441 agccctccgg ctgtatcggt tgagcaaaag tcagcgtggg aatggtgtgg tatctttatc
    10501 taagtgatac cccactttcc cattttgcta ttgattgtct agaaacatta agtttttcag
    10561 ctaattcttc ttgtgtatac tggtggattt ttcttagact ttttaaattc tcattaatca
    10621 tatttttcat acctcctatg tttataatat tggatttatt atttaaattc tattaactaa
    10681 tcttaacatt catatatata tttatatact ttttattaga taaatttatt gtttaggtaa
    10741 catacttcct taagttataa taaataaaag caagcacaaa tctatcaact tttgctaaag
    10801 tatttaagtt agagtcattt acattgttaa atacttctac accttaaaaa gttttactca
    10861 tcaatcaaat gaaaacttgc aataagaatt aaaattataa taacaaaggt gctgtctcaa
    10921 aataaagata atcttttttc attattgaaa tgataaaaat atatttgatt tcacgcaaaa
    10981 aagtgtctca tgaactaaaa agttcatttt gagacactcc ttttttataa taaaacaata
    11041 ctatctactt ctttttaact atatatctag cgcaacatga tgcccttggt atactgcatt
    11101 tgttattgta gatgcacgaa cacaatctcc aatttgagca acatatggag cacaatcaag
    11161 taaagcgtct accacttctc ttcttggttt ttgtccaagt gcacatatta ctgaagttcc
    11221 tggaatattt attttattcc cagatttatt catacatact acaccttctg ttgttacact
    11281 taaaccttta tgctctgtat atacatatat accttgtttt tctatttctt gtagtaatat
    11341 tggacgattc cttatatttg catccggggc aagttctgct ctcatctcta ctaagtgaac
    11401 agtcttccct tcttgagcca agtgaattgc agcctcacat cctgctaatc ctccacctaa
    11461 aactactact tcatcagtta ctttttcttt ttctaagtaa taatcattaa ctattactac
    11521 attttctcca tcaagacctt ctattgatgg aacaagaggt tcagacccta ctgctataat
    11581 taatgcatca acattttcat tttcaacata ttcttttgta actcttgtat ttaaacgtac
    11641 ttcaacacct aaatcttctg ctatttttcc aaaagtattt cctaattcgt acatttcata
    11701 tttaaatgga agtgcttgtt ctccttttaa aattccacct aattcattag tcttttcaca
    11761 aagtattact ttatgcccac gctttgcagc tgtaatagct gtttgtaacc ctcctggtcc
    11821 accacctgct attaatactt ttttaggttt taatactgga ataacttcag ttccatctaa
    11881 ttcacgtcca ataagtggat taacagtaca acgtcttgta gaagtggtag cacgttctgc
    11941 catacaagtg aaacatctta gacattttac tattttttca tcttgattac tcataacttt
    12001 tcttggtaat tcgtggtcag ctagtaatgc acgagccata taaataacat ctgcttttcc
    12061 actagcaatt atctcttcca tcatttctgg gtcatttaat gctcctatag tagcaactgg
    12121 aatgtttaca tgttttttaa tttcttctgc aagatataca ttacatccat gctctaagaa
    12181 catagaagga tgtgttactg caaatcctct ttgatatgta cctgctgata catgaagtaa
    12241 atctactctt gattccaata atttagcaat ttctactcca taatctaaat cgtatcctcc
    12301 ctcaaataat tcagaaccac tcattctaaa ctctattggg aatcctggac ctactgcttt
    12361 acgaacacta tctaaaactt ctttagcaaa acgaactcta ttttctaaag aaccaccata
    12421 tttatcattt cttttgttaa agtaaggtga taagaattga ttgattaacc atccatgtcc
    12481 accatgaacc ataatcatct caaaccctgc tcttttagcc aatgctgcaa catttccata
    12541 cgattctact atttcagcaa ttatttcttc tgtaagctct tttatctcta atccatcagg
    12601 acgaacacta ggacttactc cccactgtga taatcctcgc ttcttatctt tgtcaactaa
    12661 ataagttcct gaatattgtc ctgaatgaga taactctaca cttggtattg ctccatgacg
    12721 acgaattgca tctgctgtgt aagtaaaact tgctaaagag tcaactattt ttaaatctag
    12781 atgatatgca tgtgaaccat ctgtttctgg atgaaccata cattcgctaa ttgtaactgc
    12841 acctgctcca ccttttgctc ttagttcgta aaaggctgtt gattttgagc caattgtaca
    12901 atctgctgtt atgtctgttc ctcccatagg tgctgaaaac attctatttt tgaatgttac
    12961 atttccaatt ttaattggct tacatagatt tggatatttt cttttcatct tttatatctc
    13021 ccttcattaa attttctatt tttaaatttt gtattttatt tatatattta tataaaaatg
    13081 ttaatataaa acctattaaa gctataacta tagctataat gaaagccctt tgataattgc
    13141 catctgtagc atatacattt ttcataactg caggtccaac atatcctgca aaagcaaatc
    13201 caataaacat gattccataa tttacactat tatttttagg tccaaattta tctactgtaa
    13261 atcctgggaa aattcccata aatgaaccaa agcatatacc tataattatt attccaatat
    13321 agaacgttgc tacatctcct tctcttgaga ataataaaca taataaacca aaaattgaaa
    13381 taactaatgc acctgctaaa gtatttattc taccaatttt atctgagata taccctgcaa
    13441 caatacggcc taatgtatta aatagggcta aaattgaaac tactattgct gcatctgaac
    13501 ttgtcatacc aatcatattt gttgctagag aagatgctaa tgacacacac attaatccat
    13561 agaaagctcc acaagttaat attataatca ttagataaaa tataggtgtt gaaagcattt
    13621 ctttccaatt cttattaata ataactctta cttttccttt tttttctggt ggtatccatc
    13681 cttctggtgt gaaatctgct ggacattttt taataaagaa agaagaaaca catacaatta
    13741 taagaaatac taaacctatt gatttaaatg cagaggttac tccatattta ttaattatcc
    13801 aattagcaac tggaggcatt attactgaac taaacccata agctgctgtt gttataccac
    13861 caactaatcc acttttatct ggaaaaaatt taattgaatt acttattgtg catccataaa
    13921 ccatgccaac cccaagacct gttattatcc catatgataa aatcaacata ggtaatcctt
    13981 ttgaaaaacc tgccaatatc atacctatac caaaaactaa tcctcctaca aatacaacct
    14041 ttttaggacc aaatttatca tttatcattc cccctgtaat catagtaata ggtccaactg
    14101 agtttgtaac tgtaaataca attgctaaac tagcagctgt aaaagtcgtt ccagcaatac
    14161 tatttaaatg ttctgccata ggtgatgcaa atacactcca tgcatatatt gagccaatac
    14221 ataaattaat gaaacaactt gcaattaata taatccatct tttttgtgtt aaattcatta
    14281 ttctcctcct ctataaattt attaaatgga atttcttagt taagattatc acaatttttt
    14341 ctatatgtaa atataaacat gtaaatattt acatgtttat atttacataa tttctttttt
    14401 atatatagct ttatttattt ttattactca taatttacaa gttcttatta aaacatagtt
    14461 atattaatta attcaaatta aatatatcgt gttataataa gtatgtaatt aaagaaagag
    14521 gtgtatttat gcgaacttta aagtatgcaa ttttaggatt attaaataga aatcctatga
    14581 ctggttatga tatcgtaaaa gagttcaatt ttcaattagc tgagttttgg aatgcaaagc
    14641 atagtcaaat atatcctgaa ctaaaaaagc tggtaaatga aaagcttatt gtctatgata
    14701 ttaaaattag tggtgatgta ttagaaaaaa agttatacac aatcacagaa aaaggtaaag
    14761 atgagttctt gaaatggctt gaaaaggatg agccaatgga aagaactcct aaggatattt
    14821 ttcgtcttag gatgtacttt tctaataatt tagatataga tactaggctt attttactag
    14881 agcatcagtt gcttcaacac caagaaagaa ttgcgttctt atacagtcaa atggaacgtt
    14941 atagtgaagt tcctcctctt aatagtaatg attttggtga ttatatagtt cttgatggag
    15001 ctattatcag agaacaaggt gctataaaat ggctaacaaa ttgtattaac catcataaaa
    15061 atttggataa tactactttg aaataataac ttgttgattt taatagtgaa tttaattata
    15121 gatattaaat ttataaaaaa gactgtacac aactttgtct ttgtatacag tcttctatgg
    15181 ttgattattc aaaatagcta attataaaag ttaagtattt tttattaagc tattatttta
    15241 tcttcttatt acctttcaaa atctccttta tatttacaat ctttcacagt cgaattgcta
    15301 tctcttctca taatacaaat ccagttgatg tattgttact aagtttaatg gtaatcaaat
    15361 tctttaccaa taagtataaa aattactaat gtgttataat tttcttaact actaaatttt
    15421 acatgcattt gaatagaata tgtcaataaa ttactataat aaataaaaaa ttaagagtta
    15481 aaataaaaaa tagttaggga gatattatta tgattttata ttttagcggt actggtaata
    15541 gccgatatgt agctcgtaaa attgcacaag aattaaatga tgaacttata tcactaaatc
    15601 aacttataaa agaagaaaaa acagatgaat taatatcagc agataaacca ttcatattcg
    15661 tatgtcctac ttatgcatgg agattgccaa tagtggttac ggattttata aaaaaaacaa
    15721 aatttctagg aagcaacaga gtttactttg taatgacttg tggaggagat acagcaaaat
    15781 ctataaacta tattcaaaaa ctatgtaaat ataaggaatg gcaattaaaa ggtatggcag
    15841 aaattaaaat gccagagaat tatatagctt tattttctac aacagataaa gaaacatcaa
    15901 aacaaatgat taaagaagca gataaacaaa tatataggat tatttctgat atacgcaatg
    15961 aaaatgaatt tgaaactatt actcctagtg gcttaggagg tgcaattaaa agtggtataa
    16021 taaatacagt tttttataag acaattatta gtgccaaagg atttcattat actgacaaat
    16081 gcattggctg tggaaaatgt gtagagctat gtcctttaaa taatattaat ttaaaaaaca
    16141 aaaagccagt ttggaaaaat aactgcaccc attgtatggc atgtatttgt ggttgcccca
    16201 ctgaagctat tgagtataaa aataaaactc aaaatagaga aagatactat ttagaataat
    16261 atattcttaa tatttaaacc attataaaat gaactatacc cattgtttta tacagtttta
    16321 catattttaa ctgtctcatg ggtatagttc aaaagatagt gtctcaaagt gaacttttta
    16381 gttcctaaat cactcttttt tattaaatca agtatacttt aatcaccttt tcttatatca
    16441 ttttcactaa tatgttgtat tccaacaaat ataagattta ttagctctgt tagtgacttc
    16501 cattgtgatt tttttgaatc aagataataa tagtttttcg ttccttctct acgcacatta
    16561 acaatctctg cttcttttag tatcttaaga tgatgagata cggcaggacg agaaagatgt
    16621 gttttttctg tgatttctcc cactcgtata ccattaaaat cactctctaa gagagtaatt
    16681 aaaatcaact gtctagtctc atcacccata gcagagatag ctttgcgaca ttctttaaat
    16741 ttagacgtta tttcttgcat tctttgttga cattcttttt ccataaactc caccctctct
    16801 tataagtatc taacatttgc tgcttctttc tgtgcagtac gtctgtattt aacatctggg
    16861 tatccaatta ctagtgttgt aagaacatga ttactgcgtt ttagacctaa aagctttttt
    16921 aactttggcg aattatttgc tacatctgaa aaatatccac taaataaaac tcctagacca
    16981 taagactctg ccattagtga catatttgat gctgcaagtg aaccactaat cttatctttt
    17041 gtaattatca ttattgcaat aggagcattc ttaaagaaaa atttatcatc aatctcaact
    17101 tcctttgaat atttaatagc tatatttgca ataggtttta tttttctaaa aaatttcact
    17161 gcttcgtttt cataaattga ctttttattg tctaaaacaa tatatgatac atcttgtgaa
    17221 tttttagcac taggggtata cctacctgcc tcaataattt gctttacaat ttctaaagaa
    17281 acttctttgt ctttaaattt acggatactt cttctagatt taattgccat taataattca
    17341 tctgaatcta gatttggctt atttgtaagt tctattggag gttcatcaaa tcctgtcagt
    17401 gtaatagctt tttggggaca gattgctgca caatgcccac acattagaca atcttgtttt
    17461 ttaattactg actttttatt ttctataatt atattgttta ctggacaatc atttttacaa
    17521 agcccacatc caatacaaag ctctttgtca acttttatta tatgttggta tttcatattc
    17581 attcatctac ctttcgttca ttggtttaaa ctattataac acatagatat tttttatatt
    17641 cattggtaaa gaatttgatt accattaaat ttttaatctc tctattacac aaatttttca
    17701 tagaatttta aaatatcata gtttatacat aataaaaaag acaagcttaa aacctaagct
    17761 cgcctttctc ataattttaa tacaaagtaa atttcactta cttcccatac ttcctaacaa
    17821 ttttattctt taaaatttta tttgtgaatc atactatcca taaagtacaa cctaaaataa
    17881 tcctgtgtta attttctata gttaaaaaaa gttaaaaatt atacttaatt attaattttt
    17941 atactcttct tatttaattt aatacaagtt tcttatttaa tataaatttc ttgtctaata
    18001 taaatcttca ccatttgaag caattacttt cttataccat tcaaagcttt tctttttcgt
    18061 tcttcttaaa cttccattac cactattatc tctgtctaca tatataaatc cataacgctt
    18121 ttccatttca cctgtaccag cacttactaa atcaataggt gcccatgtag tataacctaa
    18181 taaatcgaca ccatctatgt aaactgcatc atgcatagct tttatatgtt ctcttagata
    18241 atcaattctg taatcatctt ctacataacc attttcgtca gctatatctt tagcacctaa
    18301 tccattttct acaataaata gtggcttttc atatctgtca tatacatgat ttaatgtaat
    18361 tcttaaacct agtgggtcaa ttggccatcc ccattgacta tatgaaagat atggattttt
    18421 agcagatgta aatatgtttc cttcagccat atcacctgca ttttcatcag ttctaacaca
    18481 acggctattg taatatgaaa atgaaataaa atctactgta tatttattta ataattcaat
    18541 atcaccagat tccatttctg gtgcaatacc tttttgctcc atatacttta atgcatagtt
    18601 agggtattta cctttagatt gtacatccac aaagaagtaa ttttctctat ctaactttgt
    18661 agcttcccat acatcattag gattgcaact ataaggatat acacttccag ctgcaaacat
    18721 acatccaact aagttatttg aatcaatttc atgtgctatt ttagttgcaa ttgcactagc
    18781 tacaagttca tgatgagcag cttgatattt tatcttttca acatcttcac cttcttcaaa
    18841 gcaaatacca gcacccataa atggagcatg taaaatcata tttatttcat taaaagttag
    18901 ccagtacttc actaatcctt tatatcttct aaagataaca ttgcataatt tctcatagaa
    18961 acctatcatt ttacgattac gccatgcacc atacttttta actaaatgca ttggacaatc
    19021 aaagtgtgta atagtgacta atggctctat tttatatttt ttacattcct taaatatatt
    19081 ttcataaaat tgaagtccct tttcatttgg tgtttcttca tcacctcttg gaaatattct
    19141 actccatgca atgctcattc tatatacttt aaatcccatt tcagcaaata actttatatc
    19201 ttctttataa ttatggtaca tatcaatacc agttttagct ggataataat gcttatcatc
    19261 aaaatcgaac atttttagtt ttcctgtaat aactgctgtt ctatcttttc ctgttgggca
    19321 tacatctaca tttgccaatc ctctttcgtc ttcattatat ccaccttcac attgattggc
    19381 tgcagtagct cctccccata aaaaatcatt cttaaaactc atagtcaaag cctcctaaaa
    19441 ttataaccaa ttcactagct tatactaatg ccttaatttt tactaaaata agaccgtaat
    19501 tagattatcc tctaattcaa tattttttct tcctgtttct actacatcta aaaattgtga
    19561 tgaatttgta ataacaattg gagttacaac actatatcct aactctttta ttgcatcaat
    19621 gtcaaaattt aatatagttt gaccttttgt aattctatca ccttgtttta tacatgcctc
    19681 aaaacatctt ccctctaact gaatagtatt taatccaata tgaataagca cttcaacacc
    19741 tgcatctgaa agaatcccta tagcatgtag actaggaaat aaggttgtaa ctactccatc
    19801 tactggagca actacttttc catcactagg tattattgct accccttgac ctagtacacc
    19861 agatgcaaat gctgcatcct ctatctcact taattttaat acttcacctt ttatagggct
    19921 tgctactatt tctctttcta atttagtaga cttgacttct tttatttctt ctttcccctt
    19981 atttcttact tttttagact cttctacttt ctcatcttta tataaaatca tagttaatac
    20041 aaaagctacg accattgata tagctatacc agaaagagct actattaagt tacccattcc
    20101 tccatctggt tcaatcattg ctggaagttc aaatattccc atacctccag ttataaactt
    20161 tctaagatta aagaatccat aaaaagctcc tccaatacca ctagcaatac aacttattat
    20221 aaatggtttc tttaaaggta ataatattcc ataaattgct ggctctgtaa ctccaaatat
    20281 accagatata aagtttggta ttgccatctc ttttagtttt ttatctttag tcttaaagaa
    20341 tattgccaac acaactgctg aacttgcaaa tgtacaagca aagaatggca tcataacatt
    20401 atcatatcct aaagtcataa cattgtttat atatacagga ataaatcccc agtgcatacc
    20461 aaaaataaca agaatttgcc acgtaaatcc tactatagca cctgctaata gtggactaaa
    20521 atctctgatt gaaattacaa cttgtgaaat aactgtagaa ccaaatgttg ctataggacc
    20581 cagaacgata agtgcaacag gcactgttat taataatgta agcattggca caaagaaaaa
    20641 cttaactaaa tctggaataa acttattaaa taatttttca cacctagatg caaaatacac
    20701 tataaatata attggtacaa ctgttccagt ataatcaatt gatacaattg gtagaccaaa
    20761 gaaatcaata tatactggtg atgcaaacat tgttccttta aataaagtgt acatcacatc
    20821 tgctcctgct gatatactgc taccttgtat agcaggataa catactgcag ctcctagtgc
    20881 taatcccaac attggtttta atccaaactt cttagctgaa gtatatccta aaaacaaagg
    20941 aaggaatgta aataatgcat ctcctgctgc atttattacc atataaccac cagatacatc
    21001 actatataac cccattgcta caaataatgc attaaatcct ttcaacatac cacaagctgc
    21061 cattattcct aatactggct gaaaaattcc tgatattata tctataccct tatcgagtat
    21121 actacctgat tttttagttt ctgaatttgc acttgagtta tctaaaccaa ttatttcaac
    21181 tacatcttta tagacagcct caacatgatt tcctataact acttgatatt gtcctccact
    21241 tttcattaca gtaacaatac cttccatatt ttttaataca ttgtcatttg ctatactttc
    21301 atcttttaaa gtaaaacgta atcttgtaat gcagtgtact aatccactaa tattttcttt
    21361 tccaccaaca ttttttacta tctcttgtgc taattgtttg tattttccca tttttctcct
    21421 ccatatttcg tttaaattaa tttttgaaat ttgaaggttt ggccaactca atgttaccat
    21481 ccaaccatag gtataatttt gttaatatca ctcctttcaa taacatttac tatatactac
    21541 tgaaaattaa tttacttata tatttgaatc tttaactact tttgctaaat gtattgttaa
    21601 atatataagc tcatcatctg ttaatatgta gttatattgt tttttaataa aatccttaat
    21661 cttaactaca cattcaaact ctttattata tttagattta ataatactta ataattcttt
    21721 atctgtccca ccttcatgtg cattatttaa aagtaatctc tgtgcaaaaa atttcaaatg
    21781 cattataaat ctataataaa acactgattc ttcatgaaat tctatcctaa aatgatacct
    21841 aactatacta agtacttctt gtattaactt tgtaatatca gatgcaaaag tatgcccctc
    21901 acttagctga gcattcacta tatgaaaagc tataaatcca gcctcatctt caggtaattt
    21961 tacgcctgtt ttattttcaa taatatctag tgccttcatt ccaatttcaa actcttcttt
    22021 ataaaatctt ttaatttccc aaaggattgc attttttaca tttattcctt ctttcattct
    22081 ttgaattgca gaatatgtat ggtcacttaa ggatatgtaa atgctttcat ttagcttctt
    22141 gtttaacttt atttgtgcat actctataat ttcacttgat aatttcatgt gctctatagg
    22201 aatattattg agtaattctt gtagtttgtt tgatacttct ttatttgata ttctaaatac
    22261 tttgtttact ttagttttat ctattagatt gccttttgat ttttgataac caattccttt
    22321 acccatgacg ataatttctt catcattatc atctaatgtg attacaacat tattgtttaa
    22381 tatcttatta attatcatga ttatcctcct tttaagcata aaaaaaactt atccataata
    22441 atgggcatac tccttcctag gatactgcta ttattaatga ataagcttag cttgtactta
    22501 aacaattact acctaattcc aattactatg ataaaacatc atacatattt tgtcaacttt
    22561 ttatttccat ataaatagat ttactacacc ttctgttata agtaatattt ctatttcttt
    22621 atgccagtgt ggtgttgtta atacttctcc ttcatttgat agactacgaa aaggaaattc
    22681 atgtattaat ttaggcattt ctaaatatat tgccatatat atcctctcct ttctagtgtc
    22741 taaaaattaa tatatactat ttaaaataat acatacttta ttttaaaaac ctcaaaatta
    22801 taatttatac aatacctaca cttcataaaa tatatctaat atttcttttt ttgtatttgc
    22861 atttaaaagt ctttcactta tatctttacc tcctaaaata tacgctattt tagatattaa
    22921 tgtcatatct tcattgtctc cagattgtgg catagcaaac ataattatta cctttacagg
    22981 taagccatct atacttggcc aatctatacc ttttttagat ttacctatta ccacagatgc
    23041 cttctttaca tattttgttt ttccatgtgg catggccatt tgataaccta tactagtaga
    23101 gcctattttt tctctacgtt ttagtgcctt tataaactta ctttttaaat ctatacttcc
    23161 tgtatcgtat aatttatcta ccaactcttg tatgatacct tcttttgtag ttgatttcaa
    23221 atctaaaata atactttctt cgcttaatat ttctctaatt tccatttgtt ttctccagta
    23281 gattattttt aaataatatt ttaatatcct ctttgctctt gcaatctatt atatagttta
    23341 tataactctt ttgcctatat aaatttgata tttccattaa aatattcttt aaaaaacttt
    23401 gattactagt ttctaaataa atatcaattg ttctattaat attattgaca ttcattatcc
    23461 ctttatttag tttactttga ttatgtatat atatatttaa atcctttcct aatgaaatag
    23521 atttatattt actccctaaa tccacgccta ttaaactttc catctgcatt acatcaaaat
    23581 taaaatatat attatcttct aaatacattc ctaataagtt tgtatctaag ttcatacttt
    23641 tcttaggttt agaaagtaag gcatttttta tattactaat atcactttcc attaatagta
    23701 ctgatacaac tatatgtaat ttatctttca agtctaggtt tacagtagat attacaaagt
    23761 ctacatcatc taaatttcga ttgctcaact gatgcatagg aactatatca actatagtcc
    23821 attcaggaaa ttctcttttt aatctggctg caagtagctg agatgttcca taacctgtat
    23881 gacaaactac tattactctt ttactactca tatttctctc tattgctgct tgaaaatatg
    23941 tggcaagata cgaaacttca tctatactaa tgttcttcag attatcatac tctgtcataa
    24001 tatttatagt catcatacat aatcccaaca catctgaaaa tcgctcttgt atctctccta
    24061 ataatggatt ttttatttgg atatcatatc ttaatctgtt taacataggt cttacatgag
    24121 atgctagact tttttgtagt aagtcatcat ttttgagatt tatatttaaa aattgcgaca
    24181 tattctctat cattatagat gaaaactttt cactttcact taatctatat gattcatcat
    24241 tgtaactatt attactttcg ctactaaatc cagatgacac taaaaaaata tatatgtaca
    24301 taatttcatc tttcgttatt tttactccgt atctattttc tattttgcct ataagtgcag
    24361 tgacattttg atatactaat tcatccaaat tatctacaga aacttctctt tcttcctgag
    24421 actctatttg attaccttct tcgattctct ttaaacatat aagtatatgg gtaattaaat
    24481 ttatatagta gggctgactt atagtgtatc ctaaattgta ttctaactct gtaattatag
    24541 tttctacaaa tataatagaa tcaatatcaa ataaatttat caacgcacta aatgtagcag
    24601 aatctaatct tgttaactta gatatatcat gctgttcata attttcatct aataaatcat
    24661 ccatcatttt ggcaatcgat ttccttatat ccacttcttt acctataatc ttagttccct
    24721 ctaaagtttt attaagtttc aaattgtatt cttttatcca tgcctctata tatttaaaat
    24781 catttactat agaagtttta ctcacataat atttatctgc taactgattt attgaagtta
    24841 tattatttga atttagtaat aaatacttta aaatctccat tcttctctgt tctactgata
    24901 aagatttatt ttccttcgat tgtatattta aactattaat taagtctata ttcttctttg
    24961 ccaaagattc tattaatatt ccatatcctc tttttatatc aatttttata ttaaaagttt
    25021 ctaagtattt ctcaataatc ttcaaatctt tttgtaaagt tttatcggat acatttaatt
    25081 tatctttaaa gtattttata ggtctatatt tattttcgtt taatatcaat ttaagaaatt
    25141 caatttgcct cgacacaagc atacttatcc ctccctacct atgtttatat atttctatgt
    25201 tcatattaag tactatttta acatatttac taactatgta aaattactaa gatacttctg
    25261 tatcataaaa gaatttatga tacaccttat taaattgata taatagaaat ataatttcaa
    25321 aataaaaaat aaggatattt agaagtagtt aactaattct aaatatcctt atttttattc
    25381 tatgtacttg tggttattct attacttatt tttactatca tttataattt aagccccaag
    25441 tacttcaact tcactatttt cactccttat agtaggcttt gttaccccta taatcaatgc
    25501 tgttattact gttccaatca atatacaagc tatatatgca actggtgaac tacatgcaaa
    25561 tggtattaca aatattcccc catgtggtgc cattaaagtt atatctaaac tcatacatat
    25621 tgccccacct atacaagaac caattatatt tgctggaatc attcttaatg ggtctgcagc
    25681 tgcaaacggt attgctcctt ctgttatgaa agatgctccc attatagcag ttggtagaga
    25741 tgactttctt tcttctttag taaacttatt tttgaatatc aacatagcta atgatatcgc
    25801 taatggaggt accattcctc ctaccattaa agctgctgat ggttcaaata tattacttga
    25861 aaacatagcc aatccaaaag ctgaagccgt tttgttaata ggccctccca tatctgatgc
    25921 catcattcct gctaatattg cacctaataa tactttatta gttccagaca tgctttgtaa
    25981 ccaagattgt aaagcttcat ttaaccatga tgctggtgta ttaactacaa agaacattat
    26041 agctccactt gtaaatgcaa tcaacagtgg acatatcaat acagcattta ctccttttaa
    26101 tacatctggt aaattttctg ttatcttcat tactcccaag gctatatatc ctgttgaaaa
    26161 acctgccacc ataccaccta taaaccctga acctccaata actgccataa aaccagctat
    26221 catagcagga gcaaaacctt gtcttcctgc tattgattta gctataaaac cagcaagtat
    26281 aggtataaat aacttaaatg ctgaatctcc tccaagttgt ccaaagaatc cagctatagg
    26341 gttgtatgtt tcatgagttg ggtctgatgc aaatatccca aacataaatg ctatggctat
    26401 accaattcca cctattacta caaatggaat catttcggat acaccactca ttaagtgctt
    26461 ataaacacct tttttctctg acccttggag tatttctttt gaatcttcta aagttcttcc
    26521 acctggttta atttctttag ccattccatt ttcagcattt ataattaaat ttttagctaa
    26581 tgaaacattc ttagcatctg ctactttctt ttgtaacact ttctttccta taaatctact
    26641 catttgaact tctttatcac aagctattat tacgtattca gctcttttta tatcttcttt
    26701 agtcaactta ttttctacac ctatcgctcc atgagtttct atcttaactt catatccaag
    26761 ttcttgcccc gcttcctcta atgctttttt tgccataaaa gtgtgagcta tccctgttgg
    26821 gcatcctgtt attccaacta taagaccttt attttgatta tcacttgaat ctcctgattc
    26881 tacagcttta ttattaaata tacttaatat ttcatatgga gtttctgcac ttagtaggtg
    26941 cttaagaaga ttttcatcca ttagatatgt agctagttta gatataatct taagatgttc
    27001 atctgagcct gactctggaa ctgctatcat aaaaaatgac ctaagttttt gaccatcatt
    27061 atccgtaatt tccttatcag ttcttaaata catcattaca ggttgattta ctacatttga
    27121 cttagcatga ggtatagcta ttaaattacc catattagtt gatgattctt tttcacgttt
    27181 attaatatca actataaact tttctttatc atttatataa ttcccatggt acaaatgatt
    27241 tgccatataa tcaatcacat ctttttttgt ttgtaattgt ttatctagta caatacaatc
    27301 gcttcgtagt atttgaccta tcataaattc tcccctcctt tttatttgtt accttataat
    27361 taattcttgt ttttataaaa gcactttaat ttatctatgg tattgaatag attcatttta
    27421 aactctataa cttctcttgc agcatctata catggtggat atatagtgtc tggctcatat
    27481 aagcttgaat ttttttctaa tacttctctg cacttcttat aatatgccga cttaacatca
    27541 cttgaaatat ttattttaca aactcctagt ttaactgctt gtgctatttc ttcatctgga
    27601 tttgatgaac caccatggag aactaaaggt atatctacta cttctcttat ttcttttaat
    27661 aaatctagtt taagctctgg tttaaaacct tttggatata taccatgtgc tgtaccaatt
    27721 gcaatagcta gtgtgtctac tcctgttttt tcaataaaat ctttagctaa ttttgaatct
    27781 gtatatatta tttcatcagt accaccttca cttgatgttc ctgttgttcc tattgttcca
    27841 agctctcctt ctacagatac atttaatcct tttgctattt ctactacttc cctagatata
    27901 gctacattct cttcatatgg taaatgtgag ccatcaatca ttactgatgt aaatccacat
    27961 cttattgctc ttaatatttg ttctttattt tccccatgat ctaagtgtat taccataggt
    28021 actttgctct tatttgcttc ttctatacaa ctagctacaa agctatcccc taggaagctt
    28081 agctcatttg ggtgtattgc aaggattact ggtgcatttt tttcgttagc actctgtact
    28141 actgctttaa gtatttgccc acttcctata ttaaatgctg gcacagcaaa ttgattttct
    28201 tgtgccactt ttagcatttc cttcatattt attaacatat tatacctccc gatattttaa
    28261 attttaggtt gaatttgatt cttgttaatt ttgttgttgt ttatatttaa ataatatcag
    28321 ggtaatgacg gttcgaaaat aacaatcgtt ttccttagtg aaaaggaact tttattatac
    28381 actcttagtt ccttttcact aaataatttt tagaagtctt tatcatagtt aattatacct
    28441 ctcatgtaca tcttttcgtc ttaagcgttt taatttaata ggctttaacc ttactatatt
    28501 tttataataa acaattaaga tactttaact ataaatttaa agatactcat gcaatagtta
    28561 atgttatatt agagtcaaaa aaattaaaaa gatattagct atttagccaa taccttttcg
    28621 tggatttcaa tagtatatac gatttatatg aatcatactc acgcttatct aatgttttac
    28681 ttccataaac tttacacaaa acaccaaaat gacaccctcc aaattcatct ctagctaata
    28741 aagtgatttg gttttctaag caccaattta taaattaacc tttattatct tatgtaaatc
    28801 tttttctatc taatttaaaa attattccta atgctatata gcatatacca attactaata
    28861 caccataagc catagatact cctatcttaa attcttgcat attagttaca atagaaattg
    28921 aaataggtct attataaaca ccataaagca aggctgacat tgtatattcc ccaattgtac
    28981 gaatgaatac taatatagct cctgaaatta ttcctggcat catatttggt actattacat
    29041 tataaaaggt ttttatcctt ccagcaccta aactccttga tgattcttct agtcctaaat
    29101 ttattgactt catagcaacc tcattggatg aaaataataa tggtagggca taaattgtat
    29161 atgctatagg tacaatataa aaacctccta ttaaggattc attaaaagca aatatacttt
    29221 tttgattaaa tgtattaatt aggttaactg caatggcgct tgcaggcata ctccaaggta
    29281 gcattataat aaattttgct agtcgattat atgtagtatc cttcttaatt actaaatacg
    29341 atactggtat agtgattatc aatcctatta ctactgccat taaagacatt ttcaagctgt
    29401 ttaatatagg ctgtaacaca cgtgattttt ggaaaatcat ctgatagttt tccagtgtaa
    29461 agtctctagg aaatatagtt gtcataattg aatgtgtgct tacaaaggat aaatacacaa
    29521 tagtaactat aggaagcatg atcataacaa tttgtatttc aataataacc ttacatacaa
    29581 atgtaaataa gtcttttttt ctattatgaa ctttataacc ttgagacttt aaggatgcta
    29641 ctactccata tttttttcca tagtactgta ataaaatcat aaatgaaatc cccattagta
    29701 acaaaataat tacttgaact gaagctatat ccatgtggtt attagcctta gacctaacaa
    29761 tctgagtgct taaaacttta aaaccaccac ctataagatt tggtgctgag aaagaactaa
    29821 tacctgatgc aaaagttata atagtagagg ttataatggc aggtgtaata acaggccata
    29881 ttacatcaaa aaatactctt atctttgatg ctcccattcc ttttgcagct tcaactgttg
    29941 aataatccac atattttaag gcaacatata catttaaata aaaatacaca tactgagtat
    30001 aggcaaccac aaataaaact ccctttaatc cactaaaatc ataaggtttt ttgataaagg
    30061 atagtaaaat ttctatagat tttgtgacta tcccactttc tccataaagc tgaatacatg
    30121 caattaccat aattactcca ggaatcatca ttggacttag taaaagaata tgtatgattt
    30181 tcttgtgttt atcacaaaca aatgtcatat ataaggcaag tgatattccc ataattccac
    30241 atacgagtac agaaccaaat cccaaaatta tagtattgct tactaccttt aaattattag
    30301 tatttgaaaa atattcttta aaaacttcaa aagaatatcc acctttacct tgaaatgctt
    30361 gtgtaaaggt attgcctatt ggtatgataa tatatccaat caaaatccaa aataatatac
    30421 tgtataaaat aatcgaaata acatttttag gtgtaaattt actacgtttt atttttttaa
    30481 tcatattagt taccttgtag cacacctaaa tatgccatat cccctacgtt taatttattg
    30541 ctattattca gtctattaag ttcagttact tgatattttt ttccattatt tgatactgta
    30601 tattctaaaa ttgctcctaa gaaattctta gattctactt ttacttttat aaaaccactt
    30661 tggttggtat tttgcaataa taccatttct tcaggtcttt tataaatata aggctttgtc
    30721 tctttttcaa tattaaaatt aacataatct tctttttcaa tacagtttga agtacctaca
    30781 aagtttgcta caaagtcatt caatggtttt ttgtaaactt ccattggaac accaatttga
    30841 agaaccttcc ccttatccat tacagctatt ctatcagaaa tagctaaggc ttcctgttga
    30901 tcatgagtaa taaataaagt agtaatacct atcttttgtt gtatctctct tatttcttcc
    30961 ctcatcttga ttcttaaagc tatatctaga ttagacattg gctcatctaa taaaagcata
    31021 gtaggttcag tcgctaatga ccttgctact gccacccttt gttgctgtcc accagataac
    31081 tcatcaatct tttttttcct aaaatctttc attcctgcca gctctaaata attatcacat
    31141 ttttccttta taattttttt agacagtttt tttattttca atccataaga tacattttct
    31201 tcaactgtca tatttggaaa cagtgcatag ttttgaaaaa cagttcccat atttctattt
    31261 tctggttcta aattggtaat atttttatca ccaacataaa tacttccaga agaaggtttt
    31321 ataaatccac caattattct taaaatagtc gtttttccac atccagaagg acctagtaaa
    31381 gtaaaaaact ctccttcctt aatagatata ttaatatcat ataatacttt ttcttttcca
    31441 taataatgat taatttcttt taattctata ttcttcatat cgtctccaca aattatctaa
    31501 tatttataaa tctccaaata tacacatgac gaatcatcat agattttaaa tctaggaaac
    31561 tctattgcca tttcatcatc cttttctagt tccctaacct ttctatatat ataatctata
    31621 cctttaatct ctgctatttt catcatttca tttttatcaa aaatcttata tctatcatat
    31681 ccacatgaaa aaccatcact agccatcata attttcattt tatattctat atcaataaat
    31741 ccacttatac aattatcaac agcctcttta gaaaattcca aagcccaata tccacctaca
    31801 gtatttttct taagtctatt ctctattata atcggcatta cctcggcttt tctctccttt
    31861 aaagtaaggt ttgtaacatc tttggcttct tgcataaaat caaatatttt tttatcaagt
    31921 ctacataaag tctcatcttt taaaattttc acagattcat tatggttaac taataaagta
    31981 caatctccta aaagtaaata ctctatctta tctttataaa actttaatat agaacatgaa
    32041 caagaaggaa tatctattgc ttctacttct ttatcatatg ttaatttgta gtactctttt
    32101 gttatctcct caatgccttc tttaataatc acttttaagc tatcttctct ttccatattc
    32161 tcatgaaggt aactattcca ccaacttaca taccatttag catcactttc tgtagagact
    32221 aaattcttat tatttaaacc tgtagctcca tcaagtaccc atgccccata aggtgataaa
    32281 cctacgatat cttcgttaaa atcgccatta cctttattac ataataattt ataatcacta
    32341 atttttagtc ccatctttat ctccttgccc acataaattg tacttttact ataattaaaa
    32401 gttttacata ataaaaaatt tactttattt aagaaactat gtctacaaat cactttatat
    32461 agtcaattac ttcgttagtt gctcaaaaac tattttattt aataaatatg tattagtatc
    32521 atttgttgta tgaattataa aaaattatga gatgctatag ttcccttata agtaaaatag
    32581 cgaggaaaat cctcactatt tttgtatata atattaaatt atttaattaa gctattttat
    32641 agttttatta ttatctatta tatctgtttc ccacttttca atccagttag actgattttt
    32701 tccaattaca gaccaatcta catccatagc tttatattct tttgacatcc actttggtga
    32761 atctttaagt gcagaatcta atgttggaat acgattaaaa tcatttgcaa ccatagcttg
    32821 aaccttagca cttccaacaa aatctacaaa tttagctgct gcatttggat gtggtgcatt
    32881 ttttagggct gctacacagt ctgtgattac tacagaacca ctctctgcat ctacaacttc
    32941 taatggcata ttattttttt ctttattctc tattatatca cttaatactg caacagatat
    33001 tgcagcttca cctttaccta ttgctgaata catgacactt ccactattgt agtaattctt
    33061 tatattttta tttaaatcct caagatactt ccaagcttcc tcttcactct tagtatttga
    33121 gtagtaattg attaagttac taatagtaga acgcatagat gatgataatg agtctctagt
    33181 aactattaaa tctttgtatt cttcttttgt taaatcgctc caatcttttg gcactttatc
    33241 agctgataat agttttgtgt tgtaaaaaat cattactggt gtttttatag ttccatacca
    33301 atatccatct gagtctttgt aagacttgtc taattcatta ttccagcttg gtttagtctt
    33361 ttcatataat ccttcttctt ttaattgcat gtatgtagat gtatctccac caaacattat
    33421 atctgcttgt ggattatctt tttcacttct aacacggtct gctaattcac cttttaagtt
    33481 tataaattca acattaactc ctgtttcctt tgtaaattct tcagaaattg ctcctagcat
    33541 atcctctcca tgtgtagtat atactactaa tgtatcctct aatttatcac tttttgatgt
    33601 gttatcattg ctacaagcta caagtgataa taacattgct acagttaata aaacaactgc
    33661 tatttttctc ttcatattga ttcctccccc atattctcca tgttcatttt aaatgttatt
    33721 attcttgtag tcaatgattc aacaacaatc tatataattg tgtttttcta tagtcactta
    33781 ttttattatt gtttttttct ataattatac ctatttgtat gcatataatt tttatattca
    33841 ccttaatcca ttttttagta tgagtatttt tctatttaaa acacacttta aatattaata
    33901 ttactatcat atcattattt aaattgtacc catcttttaa cagataacta gataaaaata
    33961 aatattgctt tatacttata tcctttgatg tatcaatatt tgacaaatca gctttcccat
    34021 tgctaataat ccctttgtat attaattcat ctcattaatt taatatgtta tctttatttc
    34081 tctataattc caagtcttta catacattat aagctacatt tttaatctat ataatattct
    34141 tatctcatta cttttatttt ctctaaataa atgtatagct ccttatatat aatctaaa
//
LOCUS       DAFNNB010000023        33811 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0023, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000023 DAFNNB010000000
VERSION     DAFNNB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33811
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0023"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            29..238
                     /locus_tag="L3M60_000992"
                     /pseudo
     CDS             29..238
                     /locus_tag="L3M60_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            405..1796
                     /locus_tag="L3M60_000993"
     CDS             405..1796
                     /locus_tag="L3M60_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225682.1"
                     /translation="MEKLARREQPHIKFGMFKIRIPFIHYRFEKPEAIQGIISSMTSL
                     GTIGLSTQILGLPYEVAWSMAIINSILYCLHVFMGDPVVPGWITASITLTTAYLLKFS
                     MGIERIQALTALQIDLGIIFILMGITGVAGKLVSKIPNSIKGGILMGVGISTIISEFS
                     PKGRFDSYPISITVGILVACFVMFSERLDTLKVKNKFLFHLGEYGVVSAILVSLIVGI
                     FSKEIEIPRFSFDNLVYIVDFKNLINTVSPFGIGFPSLILFIQGIPMAFMIYIIAFGD
                     FITGENLVLSESENRKDEYIDFNSNRSNVISGIRNIFMAIISPYIPMCGPLAATLTGS
                     VAQRYKVGKEAMQSIFSGMGTLVWVSAIFICFYPIAQIATPLIPLALSVTLLVQGYLC
                     TKLSMELCDTGVEKGLIGLMGGVIAAKGGTWGLVVGFILYFVLIDSKKRKEKDINSIE
                     DCVYEILDKKKSI"
     gene            1974..2561
                     /locus_tag="L3M60_000994"
     CDS             1974..2561
                     /locus_tag="L3M60_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6225683.1"
                     /translation="MPPKVKITKEMILNTVLDITREVGFEAVNARSIASKLQCSTRPI
                     FTCYENMNELKNEFLAFAYEYYNKYVVNYSNSKSVSPYLLLPLSYIEFAQEETHLFKL
                     LFINDLDLMMEEANDFYKEIDNEKKARLFSESIGIELECAKVIFLDLFLYTHGMAVLT
                     ATKKLTLDRYSAEKMLKNILTAFIRQEKPDWNLSI"
     gene            2594..3385
                     /locus_tag="L3M60_000995"
     CDS             2594..3385
                     /locus_tag="L3M60_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN6225684.1"
                     /translation="METKQYLIDFYNTYDEDSRLALKHGMVEFLTTMHYINKYIKSGD
                     CVLEIGAATGRYSHTLARQGYDVDAVELVEHNIEVFRKNTQSNENISIIQGNAMDLSI
                     FPDNKYDITLLLGPLYHLYNKEDKQQALHEAIRVTKPGGVVFVAYVISDGCLIDEGFH
                     RGNINVSEYIEKGLIDPQTFAAKSEPKDLFELVRKENIDDLMSVFNVTRLHYVASDGL
                     ALYMREAVDSMNDDAFALYLKYHLATCEREDLVGVTSHAIDIFRK"
     gene            complement(3566..5983)
                     /locus_tag="L3M60_000996"
     CDS             complement(3566..5983)
                     /locus_tag="L3M60_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225685.1"
                     /translation="MFRNNNRTFIRKIALNDLKINKLKTYLSGIIIMISTCLLLTVTL
                     VSYNASVDLVNASPYHAIYKSVDEKTKNILYQDKNFSSVGAYKLIGSNKKTDCIMSIV
                     YADDTAIKLMNFQPLKGNLPTNKNEIAISEKYLQEFGLDKDIGDSIKLNYYDDITNKE
                     VQCDFIIIGFLENYYQDNAKQYYTVVSNDYFKNIASTPLQNKSNTFDESIPDTVDVLV
                     KLSEENTEKDSASIKTQLKKIALSLGIKKYNIYLNDNYIESNLIDGEQIITVLVVGIV
                     VLFSSVFVIYSIFYISVVNSVQMYAKLKSLGMTSFQLKKIISLQGNILSIIFIPLGVI
                     ASCIIAYIIQPLAWQMRADLFIILILSLVMFLTVRISLFKPTRIISKISAIEAMQYTE
                     TKFRKKVKNFSYINIKNLALKNIESNRKKNLIALISLSISGVLFISIANLANSIDFKK
                     QLSQQFVYNEDYIISIKSDNLYKHITEIQQNNPLTDSLKNEIKSIHGVKKLIESKSIK
                     SNIIEPCVKDRDEKQFITLIKGITPELSKSLEEYIVSGKIDYTHLDSDSLIINKYRTK
                     SYRLNLKVGDTVTFNIFSGNDIIKKKMKIMGIIDNSNTGGMFFASDKTLESLTPYNTN
                     LDFSILIDKKHSKEIEEKLLSIIQKNSNLMLYSYEDDYRMITRAFQYIIIASYIFVGL
                     ISCFGILNMTNTLINSVLIRKKEFALLQAVGMTRKQLQNMLYREGLNISIKAICISSI
                     LGYFGSNLLCTFIKDVIRLDFINFKFSIFTILIFSFVLIGIQVLVTELLVRNIEKKSL
                     TERLRSQ"
     gene            complement(5996..6661)
                     /locus_tag="L3M60_000997"
     CDS             complement(5996..6661)
                     /locus_tag="L3M60_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225686.1"
                     /translation="MSILQVINLKKYYGQNSNITKALDSVTLYIEKGEFISIVGTSGS
                     GKSTLLNMMGGLDNPTSGKVIIKGKELSTMTDEQLTIFRRRNIGFIFQNYNLVPLLNV
                     YENIILPIGLDGETIDKDFINEIINMLSLEEKLYDMPSNLSGGQQQRVAIARALVTKP
                     AIILADEPTGNLDSRTSSDVLSLLKVTSKKFNQTIVMITHNNEIAQLTDRIIRIEDGK
                     IVN"
     gene            complement(6743..7774)
                     /locus_tag="L3M60_000998"
     CDS             complement(6743..7774)
                     /locus_tag="L3M60_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225687.1"
                     /translation="MNFKKFSCSSIYLIIAIGIIFTAILFNISIYIITDDLKLVTLLL
                     IYTIVLCGFIALIIFLLHKKIVIFCSKICHTLDSMMNSEDNIEIELEEETILSRINHR
                     LIRMYEVIQENRNSIAIEKADLQELVSDISHQIKTPIANLKMINTTLLNQTFKTEIQN
                     DFLMDMENQLDKLDFLMQSMVKTSRLETGIITLSKKKNSIYETIATALSGILFDAEKK
                     NIEVTVDCDPNLYIIHDKKWTSEAIFNILDNAVKYTNSNGKIRVTTECWVMYTKIDII
                     DNGKGISESHQAEIFKRFYREEDVHDIEGIGIGLYLSRKIISLQGGYIKVTSEIGKGS
                     TFSIFLPNK"
     gene            complement(7764..8450)
                     /locus_tag="L3M60_000999"
     CDS             complement(7764..8450)
                     /locus_tag="L3M60_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225688.1"
                     /translation="MEKILVVEDDSILNKTLSYNLIEDGYIITSKFTAKSAMKSIFEC
                     EFDLIILDINLPDKSGFELCNEIKENYNIPIIFLTANDMECDMIKGYELGALDYITKP
                     FNINVFKQKVKAFLNHFTTKTKQDYYRDGYLEINFSELSANINGNQIIFTPLEYRTLK
                     LLTENPKNILTRKVLLEKLWDIDANFVDEHTLTSVISRIRSKIEKDNLQYIKTVYGMG
                     YMWLGERNEF"
     gene            complement(8608..9672)
                     /locus_tag="L3M60_001000"
     CDS             complement(8608..9672)
                     /locus_tag="L3M60_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S ferredoxin"
                     /protein_id="HBN6225689.1"
                     /translation="MKRVDERNTMFARANYKKDSTAYNDYYKKNPDKKEIDDSIRNRP
                     NLCSEGTMTYNELNSPMASSAFDFLSDIKSLCEGKVSDTKVDVDAKSMTKKIKGLAKQ
                     YGASVVGITKLKDYHIYTNRGRHEENYGEEVNLTHKYAIVFGCEMDKEMINRAPMICE
                     VIETSKCYVDASIVGMILSYYIRNLGYDARNHMDANYLVMPVFIARDAGLGDIGRNAI
                     LTNKDYGSRLRLGVVTTDIPLLEDEYVDFGLEDFCKVCKKCSFNCPSHSLSNDIKIGD
                     DGKYNWVIEHETCYIKWRYLGTDCGMCISACPFSQNLETIKNTTSFKGNNELIQKALD
                     EYTSKFGKRIFIPGNPSWLK"
     gene            9934..10605
                     /locus_tag="L3M60_001001"
     CDS             9934..10605
                     /locus_tag="L3M60_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225690.1"
                     /translation="MNLLIIEDDINLNEGLFYAFENDGFNVFKAYTKQEGLNIFNSKN
                     IDFIILDCNLPDGDGFDVCQLIREKSDIPIIMLTARDSEIDEVKGLEIGLDDYITKPF
                     SLSVLKARVKVAIRKKSNNKVIYSNGIKLDQKLLKVYKNKVCLELSSVEYKLLSYLIE
                     NKGQILLKEQILHHIWDSEENYVDENIVSVNIRRLRVKVEDDPSNPKYIKTAYGMGYL
                     WNEVE"
     gene            10607..11515
                     /locus_tag="L3M60_001002"
     CDS             10607..11515
                     /locus_tag="L3M60_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225691.1"
                     /translation="MFILAFIICTVVIFIVSTKINQKRYDELSLMLNQILEGKEVTYP
                     DTKDTRASKISHQVKKIKDMIEIEVEQSKLEKEAIKGLISNMSHQLKTPLSNITIYCE
                     LLENINISTLQKKEFLQKMKNETFKIDWLLQSLFKMTKLEDGVIEFEVEELLIKDTLI
                     QSISTIFNKAEAKNIRVNLEPFSDIKLVHNKKWIIEAIVNVLENAIKYSPSDSTITIS
                     VIKMELYTKITIKDEGIGIDSRELNDIFKRFYRSKNVANQNGTGIGLYLTRLILEKEN
                     GNIIVESKLGSGCCFSIFLQNCKSLN"
     gene            11590..12276
                     /locus_tag="L3M60_001003"
     CDS             11590..12276
                     /locus_tag="L3M60_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225692.1"
                     /translation="MSILVTNNLVKHYGKDETKVKALNGVSIEIERATFTAIVGTSGS
                     GKSTLLNIIGGLDSPTSGDVIIKGKNISKIGKKDLTVFRRRNIGFIFQNYSLMPVLNT
                     YDNIALPVTLDKGNHIDHEYIEMLMKTLGIWDKRLKFPSELSGGQQQRVAIARALANK
                     PALILADEPTGNLDSKTTMEVVCLLKESSAKFHQTILMVTHNENIAQVCDSIIHIEDG
                     VVVNTGGEVL"
     gene            12273..14828
                     /locus_tag="L3M60_001004"
     CDS             12273..14828
                     /locus_tag="L3M60_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6225693.1"
                     /translation="MMSLVKFSIQYIKHYKKQSISIVLSIVLSVALLTGIGSLVHSAD
                     KSRIEKIREDSGDYHYFYKVDKEQLNKIKENKKSKKYTVDRLGVTKPKNQIDEPFIIE
                     FLNADSSYLDMTGRKLLKGTLPSKDGEIVLDTYSLNNLNVNKEIGTEINLDGKIYKLC
                     GILSSLSNPETAMEGFVNDYLKPKEKDSYIVYVKYDENKNIKKQNYKFMKKFNISKEK
                     AYINWPLSVEFGVKPPKNIEKYNIFEFLKNLELNTNAIILLVGVFGAAAVYSIFHVSI
                     LQRISQYGVLEVLGANNKQLLFLLFLELFLLFIVGFPIGCFLGIGVASTIYEQFPHIF
                     LSSDIVPGAFFISEKSIYLGFLFILLLLILISVRVVHQLNKYSSIESMKNFKITNTKK
                     RILESVKHSNMTKVLSHKYMTQKKGLFVGILFSLSLGGIIFLCSSYSIQLTKNNNELT
                     MKADDGLNSDYQITMQTTDFDVGIPKDKISRLNNIEGISKVYPVRYLFGAMQIKEEQL
                     FWKNFFKPLEKDYRIKTFFDGICTKQNEGGYLLKTNIYGYNNGMLEGLNSYIIDGEIN
                     THDMVKNNKVIVRLPMDGTGMYDAVDINPGDTIKVKVPKIMKPTGEIVKFKEENKNDY
                     TTKEFVVAATVKRVMANNIYFIGDYGMDIVMTNGQMKNNFNIENYNSVSIKKTEDSNS
                     VELSEDIKSVVSDVKRCIVTDYTIAIEKNNTYLNQKLLFIYGIVFVLLAISLFHIINT
                     VSYLIFSRRHEFGILRAMGITDNKFSLMMIREGFLYGFYASIIMVIGSVIGQFMIYFM
                     VKRVYLYINPILKINAPLYIGMIILNITISIVAVIIPVRQILKSDIISEINQK"
     gene            complement(15033..16232)
                     /locus_tag="L3M60_001005"
     CDS             complement(15033..16232)
                     /locus_tag="L3M60_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HBN6225694.1"
                     /translation="MDRLVGTVSRGVRAPIIRQGDDLVKIVVDSVLNASKSENFEVRD
                     KDVIAVTEAVVARAQGNYAHVDNIAKDVKDKFGDDTVGVIFPILSRNRFAICLKGIAK
                     GCRKVVLMLSYPSDEVGNHLISIDELDDKGINPWSDVLTEEKYRELFGYNKHTFTGVD
                     YVDYYKNLIKDCGAEVEIVFANNPKTILNYTTSILTCDIHTRQRTKRILRQNGAKKVY
                     SLDDIMTASVDGSGYNDQYGLLGSNKATEETVKLFPINCDEVVNKIQGNIKEITGKDV
                     EVMVYGDGAFKDPVGKIWELADPVVSPAYTKGLEGTPNEVKLKYLADNDFADLSGDEL
                     KEAISKYIVEKDNKSDDLTGNMVSQGTTPRRLTDLIGSLADLTSGSGDKGTPIIYIQG
                     YFDNYTK"
     gene            16351..17238
                     /locus_tag="L3M60_001006"
     CDS             16351..17238
                     /locus_tag="L3M60_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6225695.1"
                     /translation="MNIKLELYKVFNAVVNNKSFSLAAKELFMSQPAVSQSIKQLEEQ
                     LDTLLFYRNNKGVKLTPEGKILSEHVTTALKLISSGEDRINKFKKLEYGSLKIGVGDT
                     AARFFLLKYLEIFHKKYPHIHVSTINRTSRELISLLKDGNIDIAIINMPIEDDTLNIV
                     ECIEIHDIFVCANDYIEYKGRKISLEELNTLPLIMLENKANSRLYVNEYFLSKGIKLN
                     PDIELGSHELLLEFAYINLGVSCVIEEFSIDYLENGKLFKLDIKEPIPKRNIGYCHLK
                     DISLSLATKEFLSMISNNI"
     gene            17428..17985
                     /locus_tag="L3M60_001007"
     CDS             17428..17985
                     /locus_tag="L3M60_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6225696.1"
                     /translation="MSDSEYPQMKTLETRNCILRPASLDDVEDFFDCYKNKIVVKHLP
                     FSEHKSLEDTRKFIKSFFLNPYKKGKIGHFAIVYKKDNKVIGNMGFNNINPKALEAEI
                     GICINPSYWGHDFATEITKRVIQYGFRELNLNKIIAITYEENQNSTKSLDLLGFKCTG
                     KYIKKIHTGNTVKNVPCYQYELKKQ"
     gene            complement(18226..19086)
                     /locus_tag="L3M60_001008"
     CDS             complement(18226..19086)
                     /locus_tag="L3M60_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBN6225697.1"
                     /translation="MKKQKSPILLEMLGLFLGCISMSIGINMFLKPHTIAPGGLSGLS
                     LVLNKVTGLPVSAIMLIIGVPLVILAFRIMGTKNSLKTLFGTVVFSAIVQLTDPLSKL
                     RFTNDLLLSSIAGGILVGIGLGIMFKSDASTGGTDLIALILSKKFPGIKATKFMSCLD
                     GMVVISSGIVNRSLETALYSGIALCVLIKIADMIMEGFDHSKAFFIISDEPESLRQAI
                     TEELDRGLTILDGKGGYTRENKEVLLVVVSKKQELYLKRLVKRTDPTAFVIVTDVHEV
                     LGEGFKNLEN"
     gene            19585..20982
                     /locus_tag="L3M60_001009"
     CDS             19585..20982
                     /locus_tag="L3M60_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225698.1"
                     /translation="MHKPSYKTVFHSYIIFFMIFICLILTVIGIFLSLITIQKPNGEF
                     IRSDWPKKFTENFNEQIVFIDNKPQVKQSGLEKLRENQLWLQILDSSGNRVYGFLEPK
                     EHKDYYSNSELLTLANQNNSSNGTVYFNAIKNNKNDYIYIIYFPLNISKVNMFFDGSK
                     FVGGKSIVFSIIGIIFLIILLSGIAYGYWVTKCISNITSAVSDISKRSYLPVNIKGVF
                     TDIYDSLNILNEEIHRSDEIQNKTDIMREEWISNITHDLKTPLSPIKGYAELLTDSSI
                     NYSPEKVYKYASIMLKNIEYTNTLINDLKLTYQLKNGIIPLNKNNYDLVRLLKELSID
                     ILNYPEYESNIVEFKSNIDNINFDFDYTLLKRAFSNLIVNAFTYGDKNTKVCVHITLN
                     ERIEIIISDNGKGMSREEASNLFNRYYRGSKTKIEGTGLGLAITKQIIEVHGGNISVE
                     SILGLGSSFYIIFPI"
     gene            21202..23679
                     /locus_tag="L3M60_001010"
     CDS             21202..23679
                     /locus_tag="L3M60_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6225699.1"
                     /translation="MKIILKYIFTNIKERKIRTAVMLLSIVLSTVLLFVSFSIGLSYE
                     SAQRKMAKGMSGTATISVQSKNPDILTNLEDIPDLNAIKSKVGVLESSAIYNKGGYYE
                     EFSLIAADLSQLNKINKPRLENGDSITDFSGDKIILPNRFTSKYKIKKGDSITLQIYG
                     KSYTFQVSDIASYDTVFLRHTRGVNALLPKETLSKIINKGSGYTRVLIESEEEMTENL
                     VNKLSEELSTEKYTVSNTINETKIISDARQKIMPFFLISFFALTLSIFIIYSSYKVIT
                     LERLPFIGTFRSIGANEKTVTHILMLESILYGSIGGLIAIPIGVVVLNLMLHGLGNSL
                     EQGISIPTVISPIGVIISVIVAIIVSLFSAYIPVKKASHLPIKNIVLGTVEEKNVSNR
                     SILFIGSIMFILSVLLPRISPENTLYLAGGFSLLGLIVATIVLIPLITDIMSIVFEFV
                     YKNILGNEGKLAARNMKNNKNIIQNITLLFISISAVIAISVVGNFVKTYITDVFRDAE
                     LQGFADGKMNEEFIEDVRHMDGIKNILPLYVMNNEISGNGVTLSRLEGTDNIELYNSM
                     FAINYTNFEIKKQVTEAFKDKRSVILNEDTLKKVGLSIGDTITLSKDKYDFSYKIVGS
                     FKSRANDVEAVIPSHYAVNDFDKTNYGFLVYTAVNPDAVMIQIRDLFGDTYNWSRTVE
                     EFYNDSLNTISSFLSPMNKMTYFIFLLATVGIINNLLINYIQKRRSIAMYKSIGLSNK
                     QNIKVTLIEGFTSGLLGAVIGIVISILEIQTIFIVAGPKISMKPDLDFKTFIIVGLLG
                     IIVTLIGSIVPIIKGKKMKLIEEIKFE"
     gene            23694..24380
                     /locus_tag="L3M60_001011"
     CDS             23694..24380
                     /locus_tag="L3M60_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225700.1"
                     /translation="MKITHNSLAVEAKNIIKEYKIGNTTTRVLKEVSLQVMKGEFVSI
                     MGQSGSGKSTLLYILGGLDTPTSGKVYMNGADISHFNDEKMSIIRRRNIGFVFQFYNL
                     IPNLNVEENIMLPLLLDGKNLKDYKNQLDEILYIVGLTDRRKHTPRELSGGQQQRVAI
                     ARALIGKPEILFADEPTGNLDSKTGIEIIDLLDKINRDNGQTIIMVTHSPEAAKSSSR
                     TITVSDGLIV"
     gene            24699..25019
                     /locus_tag="L3M60_001012"
     CDS             24699..25019
                     /locus_tag="L3M60_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein HesB"
                     /protein_id="HBN6225701.1"
                     /translation="MKITLPETAIDTLKSILKDNQDKPNNIRVYFAGVGCGGPSFGLA
                     LDEKKEDDLTYEVGELQFVMSSDEYSQYGDIIIEDTGFGFRVIPENMKDQGGGGCSGC
                     SGCH"
     gene            25157..25801
                     /gene="trmB"
                     /locus_tag="L3M60_001013"
     CDS             25157..25801
                     /gene="trmB"
                     /locus_tag="L3M60_001013"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBN6225702.1"
                     /translation="MRRRRKKGADEKLLSYTKYVLRDDIDKLKGKWNLKFRNDNPIHV
                     EFGTGKGKFITTLAKQNPDINYIAMELKEEVLLKAVEKADASNLNNILFLWGDVSNIL
                     DYFEAKELSRIYINFCDPWPKNRWSKRRLTHSGFLEMYNRVLEDDGEIHFKTDNEKLF
                     EFSLNEIAANNWLLKNISLDLGNSEYENNVTTEYEDKFMSQGMRIFRCEAKKRN"
     gene            25930..26676
                     /locus_tag="L3M60_001014"
     CDS             25930..26676
                     /locus_tag="L3M60_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225703.1"
                     /translation="MWEGTMNLANLIPFIISAAALSPINSNDKVLSENQLKDIFPDAN
                     LRAVVKRYINPDEMTISNIKALDGEFYATGESISNLKGISYLENVDNFIFWNNNIKEI
                     PKEALSLKDMDSINLANNYLIDDSVVNSLSHNGVDVNCDLNFIDTKDNQYKLDSKYHN
                     VNIAKGESIDLRKIITKKIDSYYKYWEVTDNLPKDLDFIVSVSDKSVLSCEDMIIKGN
                     KTGQAVVKVILNDKNDLNTSQEVLITVNVK"
     gene            complement(26805..27083)
                     /locus_tag="L3M60_001015"
     CDS             complement(26805..27083)
                     /locus_tag="L3M60_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225704.1"
                     /translation="MVDTLEFGLKILFFILSIIWMGKIMILRTDKQIVINPLLIGISA
                     VLVMLHTSQSNIEFFGLDVQYIRIVLYIIYSLIILIGIWATNRRNGIF"
     gene            27358..27804
                     /locus_tag="L3M60_001016"
     CDS             27358..27804
                     /locus_tag="L3M60_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225705.1"
                     /translation="MESKFFNWYTQSLGGTFGIISCICAYLNGYMFVYTNSASKYFES
                     IGFLGIISSYTLLPLCMLTLIFGVIYSYTPETSDKSIFNIPFFTLNKRLILTTVVFGF
                     IGARVYFIIPAILITFNLYSNVLIAKLKYILCKNKGNDTNLKESEV"
     gene            28074..28526
                     /gene="accB"
                     /locus_tag="L3M60_001017"
     CDS             28074..28526
                     /gene="accB"
                     /locus_tag="L3M60_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBN6225706.1"
                     /translation="MNINEIKELLKAIDSTNLEYVKLESSDLRLEVSKKAQATSSPVL
                     SVQQESVVDLSLEKPVVNDTPVTSNENLSVVVAPLMGTFYDSPSPDADNFVKVGDVVE
                     EGDTLCILEAMKLMNEITSEIKGEIIEVLVSNEELVEYNQPLFKIKPL"
     gene            28542..29903
                     /gene="accC"
                     /locus_tag="L3M60_001018"
     CDS             28542..29903
                     /gene="accC"
                     /locus_tag="L3M60_001018"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBN6225707.1"
                     /translation="MIKKILVANRGDIAVRIIRTCKELGIKTVAIYSEIDKDCFHRYI
                     ADESICIGPNNISKSYNNIENIIYLALKLKCDAIHPGFGFLSENPEFAKQCEENNIIF
                     IGPTREQMILMGDKSRARETMMELNIPVVPGSESVLKTKEEALEVAREIGYPVMIKAS
                     SGGGGKGMRIVRKEEELFSNFDMASSEALAAFSNSDLYMEKFIENPRHIEVQVFGDKH
                     SNAIHLGDRDCSMQRRNQKVIEESLSPYLSDEERQKLHKIAVDIVKGVGYIGAGTIEF
                     IVDKDKNFYFIEMNTRIQVEHPVTEMVTNLDLIKLQISIANGDKIPFKQEDITFRGHA
                     IECRINAEDSSKNFAPSPGKIESLNLPGGFGVRFDTFVYAGYTIPPLYDSMIGKLICW
                     AETREECISRIYRALDEIIVEGINTNVEFQKALVTSEEFREDTHHTKFIEDVFMKKEF
                     ATL"
     gene            29918..30772
                     /locus_tag="L3M60_001019"
     CDS             29918..30772
                     /locus_tag="L3M60_001019"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HBN6225708.1"
                     /translation="MIKKFLSSKESKYVTISLDDNFKKNSVDDKFWTYCKGCDSHVFR
                     KDIEENSFVCPKCSRHYGLRARKRINLLIDKGTFMEFNSDVEFQNPLNFPKYKEKVDS
                     YKEKTKESEAVVTGYGRINGIKTVICVMNPDFMMGSMGSIVGEKITYSIEYAAENNLP
                     IIICSASGGARMQEGMVSLMQMAKTSQALSKLEEKSLPYISVLTDPTTGGVTASFAML
                     GDIIISEPNTLIGFAGPRVIEQTINQKLPEGFQTSEFLLEKGFIDMIVDRRKMKEVLY
                     QILAMHKK"
     gene            30793..31737
                     /locus_tag="L3M60_001020"
     CDS             30793..31737
                     /locus_tag="L3M60_001020"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HBN6225709.1"
                     /translation="MIENNSEKIKVLENDIKQLIIISKANNIDLSDKINSLNEKLEKL
                     KEDAFSHLSPYEKVMLSRDIKRPTTLEYIEHICSNFLELHGDRLFKDDPSIVGGIGQI
                     GKFNVTIVGHQKGRDTKENIKRNFGMPHPEGYRKALRLMKQAEKFNRPIITFIDTSGA
                     FCGLEAEERGQGEAIARNLLEMSKLSVPVITFVIGEGGSGGALGIGVGNDVCMLEHSV
                     YSVISPEGLSSILFKDSSKAKEACDVMKLTSNDLYDLKIIDKIIKEPLGGAQKDVEAV
                     SKEIKAYILERLNHYKDMDKEEIIAQRYTKFRNIGKCL"
     gene            31926..32186
                     /locus_tag="L3M60_001021"
     CDS             31926..32186
                     /locus_tag="L3M60_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HBN6225710.1"
                     /translation="MEVLKVSSKSNPNSVAGALAGVLRERGIAEIQAIGAGALNQAIK
                     SIAIARGFVAPSGMDLVCIPAFTDIEIEGDKKTAIKLIIEPR"
     gene            32339..32710
                     /locus_tag="L3M60_001022"
     CDS             32339..32710
                     /locus_tag="L3M60_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBN6225711.1"
                     /translation="MKLKLLVEEYAVCRLNNDTKIPTWIDTERFYSITRTDDELSIVC
                     LNNNIPSDVKSEKEWRILKILGPLDFSLVGILSKLSSLLADNKISIFAISTYDTDYIL
                     VKEKDIENACKILNCNGYEVE"
     gene            32739..33584
                     /locus_tag="L3M60_001023"
     CDS             32739..33584
                     /locus_tag="L3M60_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225712.1"
                     /translation="MIYIIEILKISIINIFCFIGIFIVFGLIFSITESLNNKFIFSSF
                     GKIGIIVTGAIGTFVHELSHLIMCLIFMHKINSVKFFRPIESKNDGILGYVSHSYKKD
                     NLYQSIGNFFIGIAPIIGGTVMIILIFKILLPDSYMKVTQNIDLNLYVSMINNLDISG
                     FIYIILGDISNFIRIMLLTPSVYSLKYFIFMFLMYSISTHMSLSSADLKNSLNGLSFI
                     VIVIFAISLITYLFGFDNLNISSLIVKYNIFVSLFMAIGLIFSIVTLIISYILSLVSP
                     FKNFS"
BASE COUNT        12765 a         3991 c         5135 g        11920 t
ORIGIN      
        1 ggttaaaaaa aattagaaaa aacaaaataa cgtagaatta ttaacataca ataatatggg
       61 acatccgtta ccaatttcat atattatgcc tctaaagaag actttatgta tgagtatgag
      121 tggggggatt ttttcttcag gtggaactgt agaaggaaat tcaaaaggac agtttgaatc
      181 atggaataga actattgaat tttataaaaa acctttatca agtgatttaa ataactaaaa
      241 atgaagaggt atttctatat atgcacaaaa tctatcaatt ttataaattt aatttattgt
      301 ttaaataatt tgcatgttat atactaattt taacgacatt gataatttat aaacaattat
      361 aaagttgtaa acttataaga gttattgaaa ggtggagtga agttatggaa aaactcgcta
      421 gaagggaaca acctcatata aaatttggaa tgttcaagat aagaattcca tttatacatt
      481 acaggtttga aaaaccagaa gctattcaag gtataatatc aagtatgaca agtttaggca
      541 caattggtct tagtacacaa attttaggat taccatatga agtagcatgg agtatggcta
      601 taattaattc aatattatac tgcttgcatg tttttatggg agacccagtt gtacctggct
      661 ggattacagc ttctataact cttacaactg catatttgtt aaagttcagt atgggtattg
      721 aaagaataca agcattaaca gctttacaaa tagatttagg aatcatattt attttaatgg
      781 gtataactgg tgtagctgga aaattagtta gtaagatacc aaattctatt aaaggtggaa
      841 tactaatggg tgttggtata tctactataa tatcagagtt tagtcctaaa gggagatttg
      901 actcttatcc catatctata acagtaggaa ttttggttgc ttgttttgta atgttttctg
      961 aaagacttga tactttaaaa gttaagaata aatttttatt tcatttagga gaatatggtg
     1021 tagtatcagc tattttagtg agtttaatag tgggtatatt tagtaaagaa atagaaattc
     1081 ctagatttag ttttgataat ttagtttata tagtagattt taaaaatttg ataaatacag
     1141 tatctccttt tggaataggt tttccaagct taattttatt tatacaaggg ataccaatgg
     1201 ccttcatgat atacataatt gcatttggtg attttataac aggtgaaaat ttagtacttt
     1261 ctgaaagtga aaatagaaaa gatgaatata tagatttcaa ttcaaatagg tctaatgtta
     1321 taagtgggat aagaaatatt tttatggcaa tcatttctcc atatatacca atgtgcggtc
     1381 cattagctgc gacacttaca ggaagtgttg ctcaaaggta caaggttgga aaggaagcaa
     1441 tgcagtctat ttttagtggt atgggtacat tagtatgggt ttcagcaatt tttatctgtt
     1501 tttatccaat tgcgcagatt gctacacctc ttataccatt agcgttgtca gtaacacttt
     1561 tagttcaagg atatttatgt acaaagctat caatggagct atgtgataca ggtgtagaga
     1621 agggattgat tggacttatg ggaggtgtaa ttgctgctaa aggcggaaca tggggacttg
     1681 ttgtaggttt tatattgtat ttcgttttaa ttgattctaa aaaaagaaaa gaaaaagata
     1741 ttaattcaat agaagattgt gtatatgaaa tattagataa aaagaagagt atttaaagga
     1801 aaatgtgagt tgctgttatc tattaataga ttaattgcaa ctcatgtttt atttatcagt
     1861 tttaggaatt gcaaaaaaat gcaaagattt ttcttgacat aattctttga tatgttacta
     1921 tagttatgta acgtatgtat tttattaaaa atttaatgga gaggtgaaca tatatgccac
     1981 ctaaagtaaa gataacaaaa gaaatgattt tgaatacagt cttggatatt acaagagaag
     2041 tgggatttga agctgtaaat gcgaggagta ttgcaagtaa attacagtgt tccactcgac
     2101 caatttttac ttgttatgaa aacatgaatg aattaaaaaa tgaatttctt gcttttgcat
     2161 atgaatacta taataagtat gttgttaatt atagtaattc taaaagtgta agtccttatt
     2221 tactgcttcc gctttcatac attgaatttg ctcaagaaga aactcattta tttaagttgt
     2281 tatttataaa tgatttagat ttgatgatgg aagaggcaaa tgatttttat aaagaaattg
     2341 acaatgaaaa aaaggcaaga cttttttcgg aaagcattgg aatagagttg gaatgtgcaa
     2401 aagtaatatt tttagattta tttctttata ctcatggcat ggctgtctta acagcaacaa
     2461 aaaaattaac attagataga tatagtgctg aaaaaatgtt gaaaaatata ttaacagctt
     2521 ttataagaca agaaaaacca gattggaatt tgtctattta atgtttatta actaatatag
     2581 aggaggtgaa attatggaaa caaaacaata cttaattgac ttttataata cttatgatga
     2641 agatagtcga cttgcattaa aacatggaat ggtggaattt ctcactacta tgcattacat
     2701 taataagtac ataaaatcag gtgattgtgt gcttgaaatt ggcgctgcaa ctggtcgcta
     2761 ttctcacaca ctggcacgtc agggatatga tgttgacgca gtggaattgg tcgaacataa
     2821 tatagaggtt ttccgtaaaa atactcaatc aaatgaaaat ataagcatta ttcagggtaa
     2881 tgctatggat ttatctattt tcccagataa taaatatgat attacgttac tgttaggacc
     2941 actatatcat ctttataaca aagaagataa gcaacaagct ttacatgagg caattcgtgt
     3001 aacaaaacct ggtggtgtgg tttttgttgc atatgttata tctgatggtt gtctcattga
     3061 cgaggggttc catagaggaa atattaatgt atctgaatac attgaaaaag gtttgattga
     3121 cccacagaca tttgctgcta agtctgagcc aaaggatttg tttgagcttg tacgtaaaga
     3181 aaacattgat gatttgatgt ctgtatttaa tgtaactaga ctgcattatg ttgcatcaga
     3241 tggtttggct ttatatatgc gtgaagcagt agactctatg aatgatgatg catttgcgtt
     3301 atatttaaaa tatcaccttg ctacttgtga acgtgaagat ttagtagggg ttacaagtca
     3361 tgcaattgac atatttagaa aataagtaat atgcttgttt atgtctctgg tgatgttgct
     3421 tggagatata aggaagtata tctgtataaa ataaaaacaa gtagagggac agaaaattat
     3481 gtaaagtcaa aatttcctat ccctttagct tttaaaaaat tatgttaaaa cttgtataaa
     3541 aaatggatat tttttatata cattcttact gagaacgtaa gcgttctgta agtgattttt
     3601 tttctatatt tcttacaagt aattcagtca ctaaaacttg tataccaatt aatacgaatg
     3661 aaaaaattaa aatagtaaat atagaaaatt taaaattaat aaagtctaat ctaattacat
     3721 cttttataaa agtgcaaagt agatttgaac caaaataacc tagtatactt gaaatacata
     3781 tagctttgat acttatgttt agaccttctc tataaagcat attttgcagt tgctttcttg
     3841 tcattccaac ggcttgaagt aaggcaaact ctttttttct aattaaaacg ctgtttatta
     3901 gggtattggt catatttagt ataccaaagc aagaaattaa accaacaaaa atgtatgaag
     3961 ctataattat atattgaaat gctctagtaa tcattctata atcatcttca tatgaataaa
     4021 gcattaaatt tgaatttttc tgaattatac ttaacaactt ttcttcaatt tctttagaat
     4081 gctttttatc tattaaaata gaaaaatcta aatttgtatt atatggtgtt agagattcta
     4141 aagttttgtc tgaagcaaaa aacatacctc ctgtattgga attgtcaata attcccatta
     4201 ttttcatttt ctttttaata atatcatttc cactaaaaat attaaatgta actgtatctc
     4261 caacttttaa atttaatcta taggattttg ttctatattt atttataatt aaactatctg
     4321 aatctaaatg tgtatagtct atttttccac ttacaatata ttcttcaagt gattttgaaa
     4381 gttcaggcgt aataccttta attagagtaa taaattgttt ttcatctcta tctttgacgc
     4441 aaggttctat aatattgctt ttaattgatt tggattcaat taattttttt actccatgaa
     4501 tagatttaat ttcattctta agagaatctg ttagtggatt attttgttgg atttctgtaa
     4561 tatgcttata taagttatca ctcttaatgc taattatata atcttcgtta tagacaaatt
     4621 gttgagatag ttgttttttg aaatctattg aattagcaag attagcgatt gaaataaata
     4681 aaactccact aatgcttagt gatattaatg caatcaaatt tttctttcta tttgactcga
     4741 tattctttaa tgctagattt tttatattta tataactaaa attttttact ttttttctaa
     4801 atttagtttc tgtatattgc atggcttcaa tagcagatat tttagaaata attctagttg
     4861 gtttaaataa agaaattcta acagttaaaa acatcaccag tgataaaatt aaaattataa
     4921 ataaatcagc cctcatttgc catgccaatg gttgaattat gtaagcaatt atacaagaag
     4981 ctataacccc taatggaata aaaataattg acaaaatatt tccttgtaga gaaataattt
     5041 tttttaactg aaaagaagtc atacctaacg atttcaactt agcatacatc tgaactgaat
     5101 taacaactga aatataaaaa atactatata taacaaaaac actagaaaag agaacaacta
     5161 ttccaactac taaaactgta ataatctgct caccatcaat taaatttgat tcaatatagt
     5221 tatcatttaa gtatatatta tatttcttaa tacctaaaga taaagcaatt ttctttaact
     5281 gtgtcttaat tgaagctgaa tctttctctg tattttcttc tgatagttta actaatacat
     5341 ctacagtatc aggtattgac tcgtcaaaag tattactttt attttgtaaa ggtgtagaag
     5401 ctatattctt aaaataatca tttgaaacga ctgtatagta ctgcttggca ttatcttgat
     5461 aataattttc tagaaatcca ataataataa aatcacattg aacttcttta ttcgtaatat
     5521 catcataata atttaattta atgctatctc caatatcttt atctaaacca aattcctgta
     5581 aatatttttc tgaaatagca atttcatttt tatttgtagg caagttgcct ttcaatggct
     5641 gaaaattcat aagtttaatt gctgtatcat ctgcataaac aatagacata atacaatctg
     5701 tctttttatt acttccaatt aatttgtaag ctcctacact actaaagttt ttatcttgat
     5761 atagtatatt ttttgttttt tcatctacag atttatatat agcatgatat ggagatgcat
     5821 taactaagtc tacggaagca ttatatgaaa ctaaggtcac tgttaataat aaacatgtag
     5881 aaatcattat aatgatacct gaaagatatg ttttaagctt attaattttt aaatcattta
     5941 aagctatttt tcttataaat gtgcgattat tatttctaaa catgtactac acctcctagt
     6001 taacaatttt tccatcttca atacgaataa ttctatcagt tagttgtgca atttcattat
     6061 tgtgtgtaat cattacaatt gtttggttga attttttact tgtaacttta agtaaactca
     6121 gtacatctga actagttcta ctatcaaggt tacctgttgg ttcatcagct agtataatag
     6181 ctggttttgt tacaagtgct ctagctatag caacacgttg ctgttgacca ccggaaagat
     6241 tacttggcat atcatataat ttttcttcaa gagaaagcat atttataatt tcatttataa
     6301 aatccttatc tatggtctct ccatccaatc ctattggtaa gataatattt tcgtatacat
     6361 taagtaaagg aacaagatta taattttgaa atataaaacc tatatttctg cgtctaaaga
     6421 ttgtaagctg ttcatcagtc atagtcgata actccttacc ctttataata acctttccag
     6481 atgtaggatt atctaatcct cccatcatat ttaacaatgt ggatttgcca ctcccagatg
     6541 tacctacaat agaaataaat tcgccttttt caatatatag agttacacta tcaagagctt
     6601 ttgtaatgtt tgaattttgt ccataatatt tctttaaatt aattacttgt aaaatactca
     6661 taataatacc tccttaacat cttgtaaata cagtataaaa ccaaaatgtt gcagaatcat
     6721 tacaaatgaa atatttaaaa atttatttat taggtaagaa gatagaaaaa gtcgaccctt
     6781 ttccaatctc tgatgtaact ttaatatagc caccttgaag agatataatt tttcttgaaa
     6841 gatagagtcc tattccaata ccttctatgt catgtacatc ttcttcacga taaaatcttt
     6901 taaagatttc tgcttgatga ctttctgata ttccttttcc attatcaata atatctattt
     6961 ttgtatacat aacccaacat tctgtagtta cacgtatctt accattagaa tttgtgtatt
     7021 ttacagcatt atctaatata ttaaaaatag cttcagaagt ccatttcttg tcatgaatta
     7081 tatataaatt tgggtcacaa tcaactgtaa cttcaatatt ctttttttca gcatcaaaca
     7141 aaattccact aagtgctgta gcaattgttt cataaattga atttttcttt ttagatagtg
     7201 tgattatacc tgtctctagt ctggaggttt ttaccataga ttgcattaaa aaatctaatt
     7261 tatctaattg gttttccata tccattaaaa aatcattttg aatttctgtt ttaaatgttt
     7321 gatttagtag agtagtatta atcattttta aatttgcaat aggagtttta atttgatgag
     7381 atatatcaga aactagttct tgtaaatcag ctttctctat agcaatacta ttgcgatttt
     7441 cttgaataac ttcatacatt ctaattaatc tgtgattgat acgtgataaa atagtttctt
     7501 cttctaattc aatttcaata ttgtcttcac tattcatcat actatctaaa gtgtgacata
     7561 ttttagaaca aaatataact atttttttat gaagtaaaaa tattattaat gctataaatc
     7621 cacaaagtac tattgtatat attaataata gagtaactag ttttaaatca tcagttatta
     7681 tataaattga gatattaaat aaaatagctg taaaaattat accaatagca attattaaat
     7741 aaatagaact acaagaaaat tttttaaaat tcatttcttt cacctaacca catatatccc
     7801 atgccatata ccgttttaat atactgaaga ttgtcttttt caattttgct tctaatccta
     7861 cttataacag atgttaaagt atgttcatct acaaagttag catctatatc ccagagtttt
     7921 tctaaaagta ctttccttgt taaaatattt ttaggatttt cagtaagcag ttttaaagta
     7981 cgatattcta atggtgtaaa tataatttga tttccattta tattggcaga taactcagaa
     8041 aagttaattt caagatatcc atccctatag taatcttgtt ttgtttttgt tgtaaaatga
     8101 tttaaaaaag ctttaacttt ctgtttaaat acattgatat taaatggttt tgttatataa
     8161 tccaaagctc caagttcata gcctttaatc atatcacatt ccatatcatt agcagtcaaa
     8221 aaaattattg gtatattgta attttccttt atttcattac ataattcaaa cccactttta
     8281 tctggcaaat taatatccaa aattattaaa tcaaattcac attcaaaaat agacttcatt
     8341 gcagatttag ctgtaaattt tgaagtaatt atatatccat cttcaattag gttatatgat
     8401 agagttttat ttaatataga atcatcttct actactaata ttttttccat acagttactc
     8461 cttttagttt aatattttat aatgtcaatt atagactaaa aacattgctg aattgttata
     8521 aacgagaaaa agtatttaaa agtttatata tttaataaag aacccgtcct atgataaatt
     8581 ctaagacaga ttctttatta aataaaatta tttcaaccaa gatggatttc caggtataaa
     8641 tattctttta ccaaattttg aagtatactc atctaaagct ttttgaataa gctcattatt
     8701 acccttaaat gaagtagtat tctttatagt ttcgaggttt tggctaaatg ggcaagcaga
     8761 tatacacata ccacaatctg tacctaaata tctccattta atatagcatg tctcatgttc
     8821 aatcacccaa ttatattttc catcatcacc aattttaata tcattagaaa gtgaatgaga
     8881 agggcagtta aaagagcatt ttttacagac cttacaaaaa tcttctagtc caaaatcaac
     8941 atattcatct tccaaaagag gaatatctgt agtaacaaca cctaatctaa gtctagagcc
     9001 ataatcttta tttgtaagta tagcatttct tcctatatct cctaaaccag catcacgagc
     9061 tataaaaaca ggcattacta aataattagc atccatatga tttctagcat catatcctaa
     9121 atttcttata tagtaactta aaatcatgcc gactatagat gcatccacat agcactttga
     9181 tgtttctata acttcacata tcattggagc tctatttatc atttctttat ccatctcaca
     9241 tccaaataca attgcatact tatgagttag attaacttct tcaccgtaat tttcttcatg
     9301 tctaccccta tttgtataaa tatgatagtc ttttaattta gttataccaa cgacagaagc
     9361 tccatactgc tttgcaagac cctttatttt ttttgtcatt gactttgcat ctacatctac
     9421 tttagtatca gaaacttttc cttcacataa agatttgata tcagataaaa aatcgaatgc
     9481 actacttgcc attggagagt ttaactcatt gtaagtcatt gttccttcac tacataaatt
     9541 tggtctattt cttatactat catcaatttc ttttttgtca ggatttttct tataatagtc
     9601 attataagca gtactatctt ttttgtaatt tgctcttgca aacatagtat ttctctcatc
     9661 aacccttttc aattctattc cccctagtaa aaatatattt aaaaaatata actttattgt
     9721 tgtataattt aattatataa gaacaacgtt atattttcaa tttaagtgta attaaattgg
     9781 ggaatatata attatatgct tgattataat gtttttattt tgaaaacaaa tttagataca
     9841 atctttttag tgatataaat aaaatcaaaa tatttttagt atgacaatgc aagaaaataa
     9901 ttatttatct aaaaaatcta aaggagagta aaattgaact tattaattat tgaagatgat
     9961 ataaacttaa atgaaggtct tttttatgca tttgagaatg atggatttaa tgtatttaag
    10021 gcttatacaa aacaagaagg attaaatata tttaacagta aaaatataga ttttataatc
    10081 ttagactgca atcttccaga tggagatgga tttgatgtat gtcaactaat aagggaaaag
    10141 tcagatatac caattataat gcttactgca agagactcag aaattgatga agtaaaagga
    10201 ttagaaatag ggcttgatga ttatattaca aaaccatttt ctttatctgt attaaaggca
    10261 agagtcaaag tagcaataag aaaaaaatcg aataataaag taatatattc caatggaata
    10321 aaattggacc aaaagctact aaaagtatat aaaaataaag tatgtttgga acttagctct
    10381 gtagaatata agcttctaag ttatctaatt gaaaataaag gtcaaatatt attaaaggaa
    10441 caaattttac accatatttg ggacagcgaa gaaaattatg ttgatgagaa tattgtgtct
    10501 gtaaatataa gaagacttag agtaaaagta gaggatgacc catctaatcc aaaatatata
    10561 aaaacagctt atggaatggg ttatttatgg aatgaggtag aataagatgt ttatattagc
    10621 ctttataata tgtacagttg ttatatttat agtatctact aaaataaatc aaaaaagata
    10681 tgatgaatta agtttaatgt taaatcaaat attggaaggt aaagaagtta cttatccaga
    10741 tacgaaggat acaagagcgt caaaaatttc tcaccaagta aaaaaaatta aggatatgat
    10801 agaaatagag gtagaacagt caaagttaga aaaagaagcg attaagggtt taatatcaaa
    10861 tatgtcacat caattaaaaa ctccactttc aaatataacc atttattgtg aacttctgga
    10921 gaatataaat atttccacat tacagaagaa agagttttta caaaagatga aaaatgaaac
    10981 ttttaagatt gactggttgt tacaatccct atttaaaatg accaaattag aggatggagt
    11041 tatagaattt gaagtagaag aactactaat taaagatact cttattcaga gtatcagtac
    11101 tatctttaat aaagcagaag ctaaaaatat aagggtaaat cttgaaccat tttcagatat
    11161 aaagttagtt cataataaaa agtggatcat agaagcaatt gtaaatgttt tagaaaatgc
    11221 aataaaatac tcaccatcag atagcacgat tactatttct gtaataaaaa tggaactgta
    11281 tacgaagatt actattaaag atgaaggaat aggtattgat agtagagaac taaatgatat
    11341 ttttaaaaga ttttacagaa gtaaaaatgt ggcaaaccaa aatggcacag ggattggtct
    11401 atatcttaca agattaattt tagaaaaaga aaatggaaac attatagtag aatctaaact
    11461 aggaagtgga tgttgtttta gcatcttctt acagaattgt aagagtttaa actaactttg
    11521 taggatgagt gtaattatcg tcacttacaa tattttatag aacatatatc tattgaaagg
    11581 agaaattaaa tgagtatatt agtgacgaat aatttagtaa aacactatgg aaaagatgag
    11641 actaaagtaa aagccttaaa cggagtaagt atagaaattg aacgagctac atttactgct
    11701 atagttggaa cttctggttc tggaaaaagc acattgttaa atattatagg tggattagat
    11761 agtccaacct caggtgatgt aataattaaa ggaaagaaca tatctaaaat aggaaaaaaa
    11821 gatttgacag tatttagaag aagaaatata ggatttatat ttcaaaacta cagtttaatg
    11881 ccagtattaa acacttatga taatatagct cttcctgtta cattagataa aggaaaccat
    11941 atagaccatg aatatattga gatgctgatg aagacattgg gaatttggga taagcgttta
    12001 aaatttccaa gcgaactatc tggtggtcaa caacagaggg tagcaattgc tagagcttta
    12061 gcaaataaac ctgcacttat attagcagat gaaccaacag gaaatctcga tagtaaaact
    12121 acaatggaag tggtatgttt attaaaagaa agtagtgcaa aattccatca aacgatactt
    12181 atggtaacac acaatgaaaa tatagcacaa gtatgcgact caattatcca tattgaagat
    12241 ggagttgtgg tgaatactgg tggtgaagta ctatgatgtc tctagtaaaa ttttcaattc
    12301 aatatataaa gcattataaa aaacaatcta tatctatagt tttaagcata gtactatcag
    12361 tagcattatt gacgggaatt ggttctttag ttcatagtgc agacaaaagt agaattgaaa
    12421 agataaggga agatagtgga gattatcatt atttctataa agtagataag gaacaactta
    12481 ataaaattaa agaaaataaa aaatcaaaga aatacactgt agatagactt ggtgttacaa
    12541 aacctaaaaa tcagatagat gaacctttta ttatagaatt tttaaatgct gattcttcat
    12601 atttagacat gacaggtaga aaattattaa aaggaactct tcctagtaaa gatggagaga
    12661 tagtacttga cacatattct ctaaacaatt taaatgtaaa caaagaaatt ggaacagaaa
    12721 tcaatttaga tgggaaaatc tataagttat gtggtatttt atcaagtctt tcaaacccag
    12781 aaaccgctat ggagggattc gtaaacgatt atttaaagcc taaagaaaag gattcttata
    12841 tagtttatgt aaaatatgat gaaaataaaa atattaaaaa gcaaaattat aaatttatga
    12901 aaaaattcaa catatcaaaa gaaaaagctt atataaactg gcctctttca gttgaatttg
    12961 gagtaaaacc accaaaaaat atagaaaaat ataatatatt tgagttctta aaaaatttag
    13021 aattaaatac aaatgcaatt atattacttg ttggagtgtt tggtgcagca gcagtatata
    13081 gtattttcca tgtttcaata cttcagagaa tatcacaata tggggtttta gaagtattag
    13141 gtgctaataa taaacaactt ttatttctac tatttttaga attattttta ctttttattg
    13201 ttggatttcc tataggttgc ttcctaggta ttggagtagc gtctacaata tatgaacaat
    13261 ttccacatat atttttgagt agtgatattg ttccaggagc attttttata agtgaaaaat
    13321 ctatttattt aggattttta tttatcttat tattgttaat tttaatttct gtacgtgttg
    13381 tacatcagtt gaataaatat tctagtatag aatctatgaa aaactttaag attactaata
    13441 cgaaaaagag aatattagaa agtgtaaaac attctaacat gacaaaagta ttatctcata
    13501 aatacatgac tcaaaaaaag ggattatttg ttggaatttt gttttcatta tcacttggtg
    13561 gaattatatt tctatgttct agttactcta tacagcttac taaaaacaat aatgaactta
    13621 caatgaaagc tgatgatggt ttaaattctg actaccaaat aactatgcaa acaacagatt
    13681 ttgatgtagg tataccaaaa gataaaattt caagattaaa caatattgag ggaatatcaa
    13741 aagtctatcc agtaagatat ttatttggag ctatgcaaat taaagaagaa caactatttt
    13801 ggaaaaattt ctttaaacct cttgaaaaag attacaggat aaagactttt tttgatggta
    13861 tttgtacaaa gcaaaatgaa ggcggctatc ttttaaaaac aaatatttat ggttataaca
    13921 atggcatgtt agaagggtta aattcttata ttatagatgg ggagattaat acccatgata
    13981 tggttaaaaa taataaggtt atagttagac tacctatgga tggaaccggg atgtatgatg
    14041 cagttgatat aaatccagga gatacaataa aagttaaagt gccaaagata atgaaaccaa
    14101 caggtgaaat tgttaaattt aaagaagaaa ataagaatga ttatacaaca aaagaatttg
    14161 tagttgctgc tactgtaaaa cgtgtgatgg caaataacat atatttcatt ggtgattatg
    14221 gtatggatat agttatgacg aatggacaaa tgaaaaataa ctttaatatc gaaaattata
    14281 attcagtaag tataaaaaag acagaagata gtaatagtgt ggaattgtct gaggacataa
    14341 aaagtgttgt aagtgatgta aaaagatgta tcgtaacaga ttatactatt gctattgaaa
    14401 aaaataatac ctacttaaat caaaaactct tatttatata tggtattgta tttgtacttc
    14461 ttgcaataag tttattccat ataatcaata cagtaagcta cttaattttt tcaagaagac
    14521 atgagtttgg aattttaaga gctatgggta ttacagataa taagttttca cttatgatga
    14581 taagagaagg gtttttatat ggattctatg caagtattat aatggtaatt ggaagtgtta
    14641 ttggacaatt tatgatttat tttatggtaa aacgtgtata tttatatata aatccaattc
    14701 ttaaaataaa tgcacctcta tacataggta tgattatttt aaatatcact attagtatag
    14761 ttgctgtaat tattccagta agacaaattt tgaagagtga tattatttca gaaataaatc
    14821 agaaataaat aagagttaga gttatataaa tttttaagat tatataaatt tctgacaatc
    14881 ttgtttaaat tatttaaata tatcttatct gtgaacatta aataaacctc ttaatagata
    14941 taactcttga aaagtcatat ctattaagag gttttaataa atttttatgt taatatatat
    15001 aatccaaaaa atttaaatct tattaaaaca tactattttg tatagttatc gaaatatcct
    15061 tgtatgtaaa taataggagt acccttatct ccacttccag atgttaaatc tgctaaagaa
    15121 cctataaggt ctgttaatct tcttggagta gtaccttgag aaaccatgtt tcctgttaaa
    15181 tcatcagatt tattatcttt ttcaactatg tattttgaaa ttgcttcttt caattcatca
    15241 ccagataagt cagcaaaatc attatctgct aagtatttta attttacttc gtttggagtt
    15301 ccttctaatc cttttgtata tgcaggagat actactgggt cagctaattc ccatatttta
    15361 cctacagggt ctttaaaagc tccatctcca taaaccatta cttctacatc tttccctgtg
    15421 atttctttta tattaccttg gattttgttt acaacttcat cacaatttat agggaataat
    15481 tttactgttt cttctgtagc tttgtttgaa cctaaaagtc catactggtc attataacca
    15541 ctaccatcta cacttgctgt cattatatca tcaagtgaat atactttttt agctccattt
    15601 tgtcttaaaa ttctctttgt tctttgtctt gtatgtatat cacaagttaa aatacttgtt
    15661 gtatagttaa gaatagtttt tggattgttt gcaaatacta tttctacctc tgcaccacaa
    15721 tccttgatta aattcttata ataatcaaca taatctacac cagtaaatgt atgtttatta
    15781 tatccaaata gttctctgta tttttcttct gttaaaacat ctgaccaagg atttattccc
    15841 ttatcatcaa gttcatcaat acttattaag tggtttccaa cttcgtcaga aggatagctt
    15901 aacattaata caacttttct acatcctttt gcaattcctt ttaaacatat agcaaaacga
    15961 tttctactta aaattgggaa tataacccca acagtatcat ctccaaattt atcttttaca
    16021 tcttttgcaa tattgtcaac atgagcatag tttccctgtg ctctagctac aacagcttct
    16081 gtaacggcaa ttacatcttt gtctctaact tcaaagtttt cactttttga tgcatttaaa
    16141 acagaatcaa caactatttt aactaagtca tctccttgtc ttataatagg tgctctaacc
    16201 cctcttgaaa cagttccaac taatctatcc attatctaaa atcctcctaa agaattaata
    16261 ttgtcatatt agtaccatgt atattataat tcaaatataa ttataagtaa aataaataat
    16321 actaatattt gacataagga gtaattatat atgaatataa aacttgaact ttataaggtt
    16381 tttaatgcag ttgtaaataa taagagtttt tctcttgctg ctaaggaatt gtttatgtca
    16441 cagccagctg taagtcagtc aataaagcaa ctagaagaac aattagatac tttattattt
    16501 tatagaaaca ataagggagt aaaattaact ccagagggga aaattctaag tgaacatgtc
    16561 actactgcat taaagttaat atcatcagga gaagatagaa taaataaatt taaaaaatta
    16621 gaatatggtt ctctaaaaat aggagtagga gatacagctg cacgtttttt cttgttaaaa
    16681 tatttagaaa tttttcataa gaagtatcca catatacatg tatctacaat aaatagaacg
    16741 agtagagagc ttatatcttt actcaaagat gggaatatag atatagcaat tataaacatg
    16801 ccaatagaag atgatacact aaatattgtt gagtgtattg aaatacatga tatatttgtg
    16861 tgtgcaaatg attacataga atacaaaggg agaaaaatat cattagaaga attaaataca
    16921 ttgcctttaa taatgctaga aaataaagca aattcaagac tttatgtaaa tgaatatttt
    16981 ttatcaaaag gtataaaact aaacccggat atagaattgg gttctcatga attgctttta
    17041 gaatttgcgt atataaatct aggagtatct tgtgttatag aagaatttag catagattat
    17101 ttggaaaatg ggaaattgtt taaattagat ataaaagagc caatacctaa gagaaatata
    17161 ggttactgcc atttaaaaga tatatcttta tccttagcaa ccaaagaatt tttaagcatg
    17221 atatctaata acatataatt tagaatacat atttcacaag taatttaata taaaaataga
    17281 gataagttcg taaatttgaa agattcatat agtaattagt ttttattaaa aataaaaata
    17341 aggttaattt gagaacattt atataattaa tatcgtattt taaatatgaa cagtatattt
    17401 agattaatgt ataaaggggg attagaaatg tctgattctg aatatcctca aatgaaaacc
    17461 ttagaaacta gaaattgtat actaagacct gcatcattag atgatgtcga agattttttt
    17521 gactgttata aaaataaaat agtagtaaaa catttaccat ttagtgagca taaatcttta
    17581 gaagatacaa gaaagtttat aaaatcattt tttctaaatc cctataaaaa agggaaaatt
    17641 ggacattttg ctatagtata taaaaaagac aataaagtta ttgggaatat ggggtttaat
    17701 aatataaatc ctaaagcatt agaagcagaa ataggtatat gtataaatcc aagttattgg
    17761 gggcatgatt ttgctactga gataacaaaa agagtaatac aatatggatt tagagaacta
    17821 aacttaaata aaataattgc aataacctat gaagaaaacc aaaattctac aaaatcatta
    17881 gatttattag gatttaaatg tacaggaaag tatatcaaaa aaattcatac tggaaatact
    17941 gtaaaaaatg ttccttgtta tcaatatgaa ttgaaaaaac aatagatata atcatttaat
    18001 atttatgcta gttatttaat aatgattgtt ttctaaataa aatttcaata aagtggttgc
    18061 ctcaaaatat agataagcct gttttttatt gtaaaaatac tgaaaatgca tttgatttta
    18121 tacaaaagag agtgtctcat gaaccaaaaa gtttatgaga cactctcttt gaataaatcg
    18181 tattttaatt aaaatagtat atatacttaa aatattaatt ttttattagt tctctaaatt
    18241 tttaaatcct tctccaagaa cttcatgcac atctgtaaca ataacaaatg cagtagggtc
    18301 agttctttta actaatctct ttagataaag ttcttgtttt ttagaaacta cgactaatag
    18361 aacttcttta ttttctctag tgtatccacc tttaccatca agaatagtaa gacctctatc
    18421 aagttcttca gttatagctt gtcttaaact ttcaggctca tcagaaataa taaaaaatgc
    18481 ttttgaatgg tcaaatcctt ccattatcat atctgctatt tttattaata cacaaagtgc
    18541 tataccagag taaagtgcag tttctaaact acgatttact ataccagaag atataacaac
    18601 cattccatct aggcatgaca tgaattttgt agcttttatt cctgggaact tcttactaag
    18661 tatgagtgca attaagtctg ttcctccagt agaagcatct gatttaaaca ttattcctaa
    18721 acctatacca actaggattc ctcctgcaat agaagacaat aataagtcat tagtaaatct
    18781 tagctttgat aatggatctg ttaattggac tatagctgaa aatacaactg ttccaaataa
    18841 agtttttaat gaattttttg tacccataat tctaaaagca agtatcacta atgggacacc
    18901 tataattaac atgatagctg atactggaag tcctgtaacc ttgtttagta ctaatgatag
    18961 accactaagt cctccaggag caattgtatg tggttttaaa aacatattta taccaatact
    19021 cattgatata caacccaaaa atagtccaag catttcaagt aaaattggcg acttttgttt
    19081 tttcatataa acacctccat gaacttgtca caatttaata ttataacata tttgaccagt
    19141 ttatattata taaaatttct tgatataagc aatttcaagt atttcattat tcttaatatt
    19201 tttgaatttt tatatagtgt ttttatatct ttactgatgt tgttacactc ctatgaagtt
    19261 ttttatatat tataattttt taattctttt ataaagtatc ccctataaag atggttctat
    19321 gaaaaatttc tctatactgt ttaacgtata tagaaatttt gtgtataatt ataaggtatt
    19381 taatggaaaa ttttatctta cttttatatg ttatcaaaag gatttattaa atacaacgga
    19441 cagcttgata acatataatt atataaatat aatttatgta gatagttgta tttttaaata
    19501 aatttttaca tgtcaataat tattgtctat tttatataag caacaatcaa actaaaataa
    19561 aagtttaaga aaagagttga ttaaatgcat aaacctagtt ataaaacggt atttcattca
    19621 tacattattt tttttatgat ttttatttgc ttaatcctga cagtaatagg tattttctta
    19681 tcacttatta ctattcagaa accaaatggg gaatttattc gtagtgactg gccgaaaaaa
    19741 tttacagaaa actttaatga acaaatcgta tttatagata ataagccaca agtaaaacag
    19801 tctggattag aaaagcttcg agaaaatcaa ttgtggttgc aaattttaga ttctagtgga
    19861 aatagagttt atgggttttt agaaccaaaa gagcataaag attattattc taattctgaa
    19921 ttattaacat tagctaacca aaataattca agtaatggaa ctgtatactt taatgcaatt
    19981 aaaaataata aaaatgacta tatttatatt atatattttc cattaaatat atctaaagtg
    20041 aatatgtttt ttgatggaag taaatttgta ggtggtaagt caattgtatt ttcaatcata
    20101 gggattatat ttttaataat tttgctttct ggaattgctt atggttactg ggtaacaaag
    20161 tgcatatcaa atattacatc tgctgttagt gatatttcaa aacgaagcta tcttccagtt
    20221 aatattaaag gtgtttttac tgatatatat gatagtttaa atattttaaa tgaagaaatt
    20281 catagaagtg atgagataca aaataaaact gatattatga gagaagagtg gatttcaaat
    20341 ataactcatg atttaaaaac tccactttca ccaataaaag ggtatgcaga gcttcttaca
    20401 gatagtagta ttaattattc acctgaaaaa gtatataaat atgcttcaat catgttaaag
    20461 aacatagaat atacaaatac acttataaac gatttaaaat taacctatca attaaaaaat
    20521 ggaataatcc ctttaaataa aaataactat gatttagtaa gactcttgaa agaattatcc
    20581 attgatattt taaattatcc agaatatgaa tcaaatatag ttgaatttaa gagtaatata
    20641 gataatatta actttgattt tgattataca ctattaaaac gagcttttag taatcttatt
    20701 gtcaatgctt ttacatatgg agataaaaat actaaagttt gtgtacatat aacattaaat
    20761 gaaagaattg aaatcattat atctgataat ggaaagggga tgtcaagaga agaggcatca
    20821 aatttgttta atcggtatta tagaggaagc aaaacaaaaa tagaaggaac tggtttagga
    20881 ctagcaataa caaagcaaat tattgaagtt catggaggga atatatctgt tgaaagtata
    20941 cttggattgg gtagttcatt ttatatcatc tttcctattt aattaaggtt aattaaggtc
    21001 aaataaaaaa tatattaagg ttgatagatt attatattta tatagtagtt tatcaatctt
    21061 ttttatttaa ggaggtgatt tgattaaaaa tattataaat agaatgataa aagtatggag
    21121 gagtctatta gagattcaat ttggattaaa gtataaaaat attatactga aatatcttag
    21181 ataaaggaag aaggtgtaaa tttgaaaatt atattaaagt atattttcac aaatattaaa
    21241 gaacgaaaaa ttagaacagc agttatgtta ttgtccatag ttttatcaac agttttactt
    21301 tttgtttcat tctcgattgg attatcttat gaaagtgcac aaagaaaaat ggcaaaaggt
    21361 atgtctggta cagcgactat atctgtccaa agtaaaaatc cagatatatt aaccaattta
    21421 gaagacatac ctgatttaaa tgctataaaa tcaaaggttg gtgtattgga aagctctgct
    21481 atttataata aggggggata ctatgaagaa tttagtctta tagcagcaga cttaagtcaa
    21541 ctaaataaaa tcaacaagcc acgattagaa aatggcgata gtattactga tttttctggt
    21601 gataaaatta ttcttccaaa ccgttttaca tcaaaatata aaataaaaaa aggtgattct
    21661 attactttac agatttatgg aaagtcttat acttttcaag ttagtgatat tgcatcttat
    21721 gatacagtat ttttgaggca tacaagaggt gtgaatgcac tgctacctaa agagactcta
    21781 tcaaaaatta taaataaagg cagtggatat acaagagttc ttatagagtc agaagaagaa
    21841 atgacagaaa atctagtaaa taaattatcc gaagaacttt ctactgaaaa atatacagta
    21901 tcaaatacta taaatgaaac taagataatt tcagatgcta gacaaaagat aatgcctttc
    21961 tttttaataa gtttttttgc attaaccctt agtatattta tcatatacag cagttataag
    22021 gttattacat tagaacgtct tccttttatt ggaacattta gaagtattgg tgccaatgaa
    22081 aaaactgtaa ctcatatatt gatgcttgaa agtatattat acggaagtat aggaggatta
    22141 attgctattc ctattggtgt agttgtttta aatttaatgc ttcatgggtt aggaaattct
    22201 ctcgaacaag gaatatcaat tccaacagta atatcaccca ttggagtgat tatatctgtt
    22261 atagtagcaa taatcgtttc tttatttagt gcatatatac ctgttaaaaa agctagccat
    22321 ttgccaataa aaaatattgt actaggaaca gtagaagaaa agaatgtttc aaatcgttct
    22381 atacttttta taggaagtat aatgtttatt ttatctgttt tacttccaag aatatcccct
    22441 gaaaatacat tgtatctagc tggcggattt tcattactag gattaattgt agctactatt
    22501 gtgttgattc ctcttattac tgatatcatg tctatagttt ttgagtttgt ttataaaaat
    22561 attttaggga atgaaggtaa attagctgct agaaatatga aaaataacaa aaatataata
    22621 cagaatatca ctttactatt tataagtatt tctgcagtta ttgcaataag cgttgttggt
    22681 aactttgtca aaacttatat aacagatgtg tttcgtgacg cagaattaca aggttttgca
    22741 gatggaaaga tgaatgaaga gtttattgaa gatgttagac atatggatgg aattaaaaac
    22801 atattacctc tttatgttat gaataatgag atttctggta atggtgtaac attatcaaga
    22861 ctagagggta cagataatat agaactatat aactctatgt tcgcaatcaa ttatacaaat
    22921 tttgagataa aaaaacaagt aacagaagct tttaaggata aacgttctgt tattttgaat
    22981 gaagatactc taaaaaaagt agggttatct ataggagata caataactct atctaaagat
    23041 aagtatgatt tttcttataa aatagtaggt agttttaaat ctcgtgcaaa tgatgtagaa
    23101 gctgttatac catcacatta tgcagttaat gattttgata aaactaatta tggtttttta
    23161 gtatatacag cagtcaatcc agatgctgtt atgattcaga taagggattt atttggagat
    23221 acatataatt ggagtcgtac agttgaagaa ttttataatg attctttaaa tactataagt
    23281 agttttttgt ctcctatgaa taagatgacc tatttcattt ttcttcttgc tacagtaggt
    23341 attattaata atctactgat taattatatt caaaaaagac gttctatagc tatgtataaa
    23401 tctataggac taagtaataa acaaaatata aaagtgacac tgattgaagg gtttacttca
    23461 ggtttacttg gagctgttat tggtattgta atatctatac ttgagattca aactatcttt
    23521 atagttgcag gaccaaaaat ttcaatgaaa ccagatttag attttaaaac atttattatt
    23581 gttgggttat tgggaattat agtaacttta ataggttcga tagtgcctat tataaaaggg
    23641 aagaaaatga aactgataga agaaattaag tttgaataat tatggaggtt aatatgaaaa
    23701 ttacacataa tagtttagct gtagaagcaa aaaatattat caaagaatat aaaattggaa
    23761 atacaacaac tcgagtgtta aaagaggtgt ctttacaagt aatgaaaggt gaatttgttt
    23821 ctatcatggg tcaatctggt tctggtaaaa gtactctttt atatatttta ggtggtttag
    23881 atactccaac gagtggaaaa gtgtatatga atggagctga tatatcacat tttaatgatg
    23941 agaaaatgag cataatacgt cgtagaaata taggattcgt atttcaattt tataacttaa
    24001 taccaaattt aaatgtggag gaaaatataa tgcttccttt gctattagat ggtaaaaatt
    24061 taaaagatta taaaaatcaa ttagatgaaa tcttatatat agttggttta actgatagac
    24121 gtaaacatac accacgagag ttatctggtg gtcagcaaca acgtgttgct atagctcgag
    24181 ctttaattgg aaaaccagag attttatttg ctgatgagcc tactggaaat ttagatagta
    24241 aaacagggat agaaataata gatttactgg ataaaataaa cagggacaat ggacaaacta
    24301 taattatggt aacacactca ccagaagcag ccaaaagtag tagtcgtact attacagttt
    24361 cagatggttt aattgtataa gggctaatta ttgtatttga gataatcatt taaagtatat
    24421 aaattttgca tatatgaatt ggacattatc aaatgtgtta taatattaaa acaacaaata
    24481 aaattattat attttttaga ataataattt tatttgttta ctaagtttat gatagggtat
    24541 ttatttaagg taataaaata ttatataaaa tattttaaag ccttaggtaa tattaggtag
    24601 aagttttagt aaaatatgca aaaactaatt gattatgtac cttgtatggg tatataatag
    24661 atattgtttt aataaaatat ttgaaaggga atgatttgat gaaaattact ttaccagaaa
    24721 cagctatcga tactttaaaa agtatcttaa aagacaatca agataaacct aataatataa
    24781 gagtatactt tgcaggtgtt ggttgtggag gtccttcttt tggattagct ttagatgaga
    24841 aaaaagaaga tgacttaact tatgaagttg gagaattaca atttgtaatg agctctgatg
    24901 aatatagtca gtatggagat ataataatag aagatacagg atttggattt agagttattc
    24961 ctgaaaatat gaaagatcaa ggtggaggag gatgctctgg ttgttcaggg tgccactaag
    25021 attaagtttt tagtaagttt agaactctct ttatagaaat aaatttctat tttaagagag
    25081 ttctttttat tttttggtga gataatgtat aataaaaatg tattttacta attttttata
    25141 tgtaaggaga attattgtga gaagaagacg aaaaaaaggt gcagatgaaa agcttttaag
    25201 ttatacaaaa tatgttttaa gagacgatat agacaaatta aaaggcaagt ggaatttaaa
    25261 gtttagaaat gataatccaa tacatgttga atttgggact ggaaaaggta agtttataac
    25321 aactttagct aagcagaacc ctgacataaa ttatattgcc atggaactta aggaagaagt
    25381 tcttttaaag gctgttgaaa aagcagatgc ttctaatttg aataatatac tatttttatg
    25441 gggagatgta agcaatattt tggattactt tgaagctaaa gaattatcga gaatttatat
    25501 aaatttctgt gacccatggc ctaaaaatag atggagtaaa agaagactca ctcattctgg
    25561 atttttagaa atgtacaata gggttttgga agatgatggc gaaattcact ttaaaacaga
    25621 taatgaaaaa ttattcgagt ttagtttaaa tgaaattgct gcaaataatt ggctattaaa
    25681 gaatatttct cttgatttag gaaatagtga gtatgaaaat aatgttacaa cagagtatga
    25741 agacaaattt atgtcacaag gtatgcgaat atttagatgt gaagctaaaa aacgtaacta
    25801 gtaatatgtt tataagttta atagtagaaa tttattttaa tttatattaa taaagtgtac
    25861 gaaagtaggc atatataaca aggaaacaga accctctcat agaaattaaa ttattataat
    25921 aaaaaaatta tgtgggaggg tactatgaat ttagctaatt taataccatt tattatttct
    25981 gcagcagctt taagtccaat aaattcaaat gataaggttt tgagcgaaaa tcaacttaaa
    26041 gatatttttc ctgatgcaaa tttaagagca gttgtaaaaa gatatataaa cccagatgag
    26101 atgactataa gcaatataaa ggctttagat ggagaatttt atgcaactgg tgagagcatt
    26161 tcaaatttaa aaggaatatc atatcttgaa aatgtagaca attttatttt ttggaacaat
    26221 aatataaaag agattcctaa agaagcttta agtttaaaag atatggattc aataaatctt
    26281 gccaataatt atttgattga tgacagtgta gtaaattctc tttctcataa tggtgtggat
    26341 gtaaattgcg atttaaactt tatcgataca aaagataatc agtataaact ggactctaaa
    26401 tatcataatg ttaatatagc aaaaggtgaa agtattgatt tgagaaaaat aattacgaag
    26461 aaaattgata gctattataa atactgggaa gttactgata accttccaaa agatttagat
    26521 tttattgtat ctgtaagtga taagagtgta ctttcatgtg aggatatgat aatcaaaggt
    26581 aataaaacag gtcaagcagt agtaaaagtt atactaaacg acaaaaatga tttaaataca
    26641 tctcaagaag ttctaattac tgtcaatgta aaataatctg tcaacttatt tagtcattct
    26701 attttcaatt taagtgtata atcaaaataa aggttgctag taacagaaca ctaatattag
    26761 cgctttgtta ctagcaacct ttaaatttta tagaaataga ttatttaaaa aattccattt
    26821 cttctgttag tagcccatat tcctattaaa attatcagtg aatatattat atacaaaact
    26881 attcttatat actggacatc taatccaaaa aattcaatgt tactctgtga ggtatgcaac
    26941 atcacaagga ctgcagatat accaataagc aaaggattta ttactatttg cttatcagtt
    27001 cttaaaatca ttattttacc catccaaatt attgataata taaaaaataa aattttaaga
    27061 ccaaattcta atgtatctac caaagcgaaa acatcccttc atttataata aatttttcag
    27121 ctcaacaaaa tataaaaccc taagaacaac ttttaaatct ataataatat agcttgatta
    27181 agcactataa aataagtacc ataaaatttt atctacatta atatatacta cactatactt
    27241 caaatttctt aaacataaat taatgaaatt aacaaaaatt tattcataag cacataaaga
    27301 tatatttcat tatttaaatg catatcatta tatatacagt tttaggaggt gaggtatatg
    27361 gaatctaaat tttttaattg gtacactcaa tccttagggg gaacttttgg cattatatca
    27421 tgtatatgcg cctacttaaa tggatacatg tttgtttata caaatagtgc tagcaaatac
    27481 tttgaaagta taggatttct gggaataatt tcaagttata cactacttcc actttgtatg
    27541 ttgactttga tttttggagt tatatattct tatactccag aaacatcaga caaaagtatt
    27601 tttaatatac ctttttttac tttaaataaa agattaatac ttactacagt agtttttggt
    27661 tttataggtg ctagagttta ctttataatt cctgcgattt taataacttt taacctatat
    27721 agtaatgttt taatagctaa attaaagtat atcctatgta agaataaagg aaatgatact
    27781 aatttgaaag aaagtgaagt ctaaattgca taatattggt atttgtctta aaaattcccg
    27841 aggtattaat ttgtattctt ttgtattttg caaaaaatat tgaattttat tcattttata
    27901 cttataattt atattaataa tggaaaatta aaacaaatat aagttactgg ttgttaattt
    27961 aaaattaaaa gatatattga tgtgttgatt gtctagtcag cactttttct tttataaatt
    28021 tgttttttgt gatataatat tagcaccagg taataataca agggggtaac aaaatgaaca
    28081 tcaatgaaat taaggaatta ttaaaggcca tagactcaac taacttagaa tatgtaaaac
    28141 ttgaaagtag cgatttaaga ttagaagtat ctaaaaaagc tcaagctaca agttcacctg
    28201 ttttaagtgt acaacaagaa tcagtagtgg atctttcttt agaaaagcca gttgtaaacg
    28261 atacacctgt cacatctaat gagaatctat cagtagtggt agctcctttg atgggaacat
    28321 tctatgattc tccaagtcca gatgcagata attttgttaa ggttggcgat gtagttgaag
    28381 aaggcgacac tttatgtata ttagaagcaa tgaagcttat gaatgagata acaagtgaaa
    28441 ttaaaggtga aataattgaa gtattagtta gtaatgaaga actagtagaa tataatcaac
    28501 ctttatttaa gataaaacca ttgtagtagg aggtatttaa gttgataaaa aaaatactag
    28561 ttgcaaatag aggcgatata gcagttagaa tcatcagaac atgtaaagaa ctaggtataa
    28621 aaactgttgc tatctattct gaaatagaca aagattgttt tcatagatat atagctgatg
    28681 aatctatttg tataggacca aataatataa gtaagagtta taataatata gaaaatataa
    28741 tataccttgc tcttaaatta aagtgtgatg ctattcatcc aggctttgga tttttatcag
    28801 agaatcccga atttgcaaaa caatgtgaag agaacaatat tatttttata ggtccaacaa
    28861 gagaacaaat gattttgatg ggagataaat ctagagcaag agaaactatg atggaattaa
    28921 atatccctgt tgttcctggt tcagaatctg ttttgaaaac taaggaagaa gctttagaag
    28981 ttgcaagaga aattggatat cctgttatga taaaagcctc aagtggaggc ggtggaaaag
    29041 gtatgagaat cgttagaaaa gaagaagaac ttttttcaaa ttttgatatg gcaagttcag
    29101 aagctttagc agcattttct aatagtgatt tatatatgga aaaatttata gaaaatccta
    29161 gacatataga agtacaagta tttggagata aacattcaaa tgcaatacat ttaggtgata
    29221 gagattgttc tatgcaaagg cgaaatcaaa aagtaataga agaatcctta agcccatacc
    29281 tttcagatga agaaagacaa aaacttcata aaattgcagt agatatagta aaaggtgtag
    29341 gttatatagg tgcaggtact attgagttta tagttgacaa agataaaaac ttttatttta
    29401 tagagatgaa tacaagaata caagttgaac atccagttac agagatggta acaaatcttg
    29461 accttataaa acttcaaata tctatagcta atggggataa aattcctttt aaacaagaag
    29521 atattacttt tagaggtcat gctattgaat gtagaataaa tgcagaagat tcatcaaaga
    29581 attttgctcc atcaccaggt aagatagaat cattaaattt acctggaggt tttggtgtta
    29641 gatttgatac ttttgtatat gcaggatata ctataccacc tttatatgat tctatgatag
    29701 gtaagttaat atgctgggct gaaactagag aagaatgtat aagtagaata tatagagctc
    29761 tagacgaaat tattgtagaa ggtattaata cgaatgtaga atttcaaaag gcacttgtaa
    29821 caagtgagga gtttagagaa gatactcatc ataccaaatt tatagaagat gtctttatga
    29881 agaaagaatt tgctacatta taaggagtaa atagattatg ataaaaaaat ttttatcatc
    29941 aaaagaatct aagtatgtta cgatttcttt agatgacaac tttaaaaaga atagtgtaga
    30001 tgataaattt tggacctatt gcaaaggttg tgatagtcat gtatttagaa aggacataga
    30061 agaaaattca tttgtatgtc caaaatgttc aagacattat ggcttaagag caagaaaaag
    30121 aataaattta ttgattgata aaggaacttt tatggagttt aattctgatg tagaatttca
    30181 aaatccacta aattttccta aatacaaaga aaaagtagac tcttataagg aaaaaacaaa
    30241 agagtcagaa gctgttgtca caggttatgg aagaataaat ggaattaaaa cagttatttg
    30301 tgtaatgaat cctgatttta tgatgggaag tatgggttct attgttggag aaaaaataac
    30361 ttattcaata gagtatgctg ctgaaaataa tttacctatc attatttgtt ctgcttcggg
    30421 aggagctaga atgcaagaag gtatggtatc tcttatgcag atggcaaaga cttctcaagc
    30481 gctttctaaa cttgaagaaa aatcattacc atatatatca gttctaactg accctactac
    30541 tggaggagta actgctagtt ttgctatgct tggagatatt ataatttctg agccaaatac
    30601 tttgataggt tttgcaggtc caagagtaat agaacaaaca ataaatcaaa aacttcctga
    30661 aggatttcaa acttctgagt ttttacttga aaaaggtttt attgacatga tagtagatag
    30721 aagaaaaatg aaggaagtat tatatcaaat tcttgctatg cacaagaaat agggcataaa
    30781 ggaggaactg ttttgattga aaataatagt gaaaaaataa aagtcttaga aaatgatatt
    30841 aaacaattaa taattatttc taaggcaaat aatatagatt taagtgataa aataaattca
    30901 cttaatgaga aattggagaa gcttaaagaa gatgcttttt cacatttatc accatatgaa
    30961 aaggtaatgc taagtagaga tataaaaaga cctacgacct tggaatacat agaacatatt
    31021 tgttctaatt ttttagagct tcatggtgat aggcttttta aagatgaccc ttcgatagtt
    31081 ggaggaattg gtcagatagg aaaatttaat gttactatag ttggtcacca aaaaggaaga
    31141 gatactaaag aaaatataaa aagaaatttt ggaatgcctc atccagaggg atatagaaaa
    31201 gcattaagac ttatgaaaca agctgaaaaa tttaatagac ctataataac gtttatcgat
    31261 acatcaggag ctttttgtgg tctagaagcg gaagaaagag gacaaggaga agctattgca
    31321 agaaacttac ttgaaatgag taaactttct gttccagtaa tcacttttgt aattggtgaa
    31381 ggtggtagtg gaggagcttt gggtattgga gtaggaaatg acgtttgtat gttagaacat
    31441 tcagtttatt ctgtgatttc tccagaagga ctttctagta tcctatttaa agattcgtct
    31501 aaagctaaag aggcttgtga tgttatgaaa cttacaagta atgatttata tgatttaaaa
    31561 ataatagata agattataaa agaacctctt ggaggagcac aaaaagatgt agaggctgtt
    31621 tcaaaagaaa taaaagctta tattttagaa agattaaatc attacaaaga catggataaa
    31681 gaggaaatta tagctcaaag atatactaaa tttagaaata ttggaaaatg cttatagaat
    31741 ataaagaaat ttgtagtttt ttcattataa aatggttaaa aaaaataaag gttttcttaa
    31801 aatgattata gaagtatatt tttataaaag tatactttaa atgatgattg taattagtaa
    31861 atggcaatta tgatttagta ctttaggtaa tggatacttt aacaaattaa caaggagatg
    31921 ttgaaatgga agtactaaaa gtttcatcaa aatctaatcc taattctgta gctggagcct
    31981 tggctggagt actgagagaa cgaggcattg cagaaataca agctatagga gctggagctc
    32041 taaatcaagc gataaaatca attgcaatag caagaggttt tgttgcacct agtggaatgg
    32101 atttagtttg cattcccgca tttactgata tagaaatcga aggtgataag aaaacagcca
    32161 ttaaacttat aattgaacct agataatata tctctttatt agaatttaat taagttttaa
    32221 aggttggcta tagaaatagg acactatagt gaaccttttt tactttatct gttttatttt
    32281 atgtaacttt taatgtataa tcttaagtaa acaaataaga ttggaggaaa tatacattat
    32341 gaaattaaaa ttattggtag aagagtatgc agtatgtaga ttaaataatg atactaaaat
    32401 accaacatgg atagatacag agaggtttta ctctataact agaacagatg atgaattaag
    32461 tattgtatgc ttaaataata atattccttc tgatgttaag tcagaaaagg agtggagaat
    32521 acttaagata ttaggtcctt tagacttttc acttgtagga attttatcta agttaagtag
    32581 tttgttagca gataataaaa taagcatatt tgcaatttct acatatgata cagactatat
    32641 attagtaaaa gaaaaagata tagaaaatgc ttgtaaaatt ttaaactgta atggatatga
    32701 agtagagtag ttaaatactt ttacatgagg tttaacttat gatttatata atcgaaattt
    32761 taaaaataag tataataaat attttttgtt tcataggaat atttattgta tttggattga
    32821 tttttagcat tacagaaagt ctaaataata aatttatctt ttcatctttt ggaaaaattg
    32881 gaattattgt aactggagct ataggaacgt ttgttcatga attgagtcat ttgataatgt
    32941 gtttgatttt tatgcataaa ataaattcag ttaagttttt cagaccaata gaaagtaaaa
    33001 atgatggtat actaggatat gtaagtcata gttataaaaa agataatctt tatcaaagta
    33061 taggaaattt ctttatagga attgcaccta ttataggtgg aacagtaatg attattttaa
    33121 tttttaagat attattgcca gatagttata tgaaagtgac tcaaaatata gatttaaatt
    33181 tatatgtaag tatgattaat aatttagaca taagtggttt tatatacatt attttaggcg
    33241 atatttctaa ttttataaga ataatgttgt taacacctag tgtatattca ttaaagtatt
    33301 ttatatttat gtttttaatg tattctatat ctacacatat gtcacttagt agtgctgatt
    33361 tgaaaaatag tttaaatggt ttgagtttta tagtcatagt tatttttgca ataagtctaa
    33421 ttacatactt atttggattt gataacttaa atatatccag tttaatagtt aaatacaata
    33481 tatttgtaag tttatttatg gcaatagggc ttattttttc aatagtaact cttataatat
    33541 cctatatttt aagcttggtt agtccattta aaaactttag ttaaataaat acatatttta
    33601 ctaatatttg gttttgttaa aacatataaa gatattatat aaaaaaagat tttagtagag
    33661 cataaaaaat ttctcaatat attttaaaat atttctataa atatattgag aaataaaaaa
    33721 agagtactca aactcaacta gtctttaaat gtggatttag ggaatagtta gtaagaattt
    33781 cgattaaagc actgctaatc tcttaaaaat g
//
LOCUS       DAFNNB010000024        33702 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0024, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000024 DAFNNB010000000
VERSION     DAFNNB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33702
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0024"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(53..1981)
                     /locus_tag="L3M60_001024"
     CDS             complement(53..1981)
                     /locus_tag="L3M60_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6225713.1"
                     /translation="MHKRLLTLFKLLNESDDKITCKTLSNHLKVSERTIRNDITSINE
                     TLEKNGAIIKIKKGEGYYIDILNLALYQHYLALISDDIMDSSEIPDSPIERNQYILKY
                     ILYNNTYIKLEDLANSLYVSKFTILNDIKRIKPILSKYNLILVSKPYYGVKVEGKEID
                     IRRCISNNMINRNFENYIIGITDREIELFNNVDLIELQKIVLSEINKFNINFLDFNLK
                     NFIIHLSITISRILGGYCLDNVLDVVLTDFQSNTAVENIFNYIESKYTIIISKADRVY
                     LYNHFITKSSLLDNVSNRADTKIIEYVEEILEVINNQYTFDLRNDSVLFDDLVLHFKS
                     ILNSKSYNLNKVNPLINTIKSNYPLAFEISLNAIEKVFKNSIYSLTEDEIGYVSLHIG
                     AGIERFFQNNIKCKNVVLVCGSGYGSSRLLEVQLNKVFHDKINILQCLSFNQFLASEL
                     SDVDIIISTIPLNHDSIPIVLVDLKLLKKDIENISKSITNNSRIYSNLLDNFFDKNLF
                     IVNPKIKEKDELIKLMCNKLTQSEIVIPSFTESVFYRESLSSTNIDDFLAIPHPMELS
                     SIKTKICISILNEPIYWSEDSTVKLIMMLAINKDDYIKINSIYDILLKIIHDNDIRDS
                     ISNCNDFDSFLSIIKSII"
     gene            2216..2668
                     /locus_tag="L3M60_001025"
     CDS             2216..2668
                     /locus_tag="L3M60_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225714.1"
                     /translation="MVLQEKNIFIDKDLKSKEEVLNFIADSAYILGITTNKDALLKDL
                     LKRESEFPTGIRDGFAIPHTKSANVKEASILYIKTTNELEWKTLDDSKVRYVFSLLAP
                     HENEGNIHLKTLSKLATCLMEDDFISEVKNASDASHLVKYITNKMKEE"
     gene            2671..2979
                     /locus_tag="L3M60_001026"
     CDS             2671..2979
                     /locus_tag="L3M60_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBN6225715.1"
                     /translation="MKIVAITSCIAGLAHTPMAAKALEQAASKMGHKIKVEQQGAMGR
                     VNTITKEEAQDADFILIASDQTISGMERFDGKKVIKVKIGVALKKPEAVLTKCIETIS
                     "
     gene            3094..4200
                     /locus_tag="L3M60_001027"
     CDS             3094..4200
                     /locus_tag="L3M60_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBN6225716.1"
                     /translation="MSKKNNSLLSELKGHLMTGISYMLPLVIGSSLVVAIPKLTALAM
                     GITSLDAYKNVSGFYHILYLMEQVGWTGIGLLNTVLAGFIAFSIGEKPSMGAGFIGGL
                     IASNTNAGFLGAVTAGFFAGYICKFLKKKIKISGSAAGMMPLIIMPLITVGLTGFLMS
                     VVLAGPLGNINTSLNAWVAEMCQNGTNSVVLALILGSMIGFDLGGPVNKAAWMAGNAL
                     LLEGIYLPAILVNCAIVIPPLGYGLATFIRKSRFSKTLSETGKGNIVMGIIGITEGAI
                     PFTLKNPLKLVPVNMIGCALGVGLSALLGVHAIMPPVGGLYGFISVGSGWAYLVGAIF
                     GALVIAILSTILVDFNEDETAENTEFDEIELDIM"
     gene            4274..6952
                     /gene="mngB"
                     /locus_tag="L3M60_001028"
     CDS             4274..6952
                     /gene="mngB"
                     /locus_tag="L3M60_001028"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HBN6225717.1"
                     /translation="MKKTKVHVVPHSHWDREWYFTTSRSKIYLMKDFQDILDILEEKE
                     DFKYFTMDAQASLLDDYLKWRPQDEYRIRDLVQRKKLAIGPWYTQTDQLVISGESIVR
                     NLYYGIDRCNEFGEPMRVGYVPDSFGQSAQMPQIYRGFGIDSSLFWRGVSDDMVDTTE
                     FIWKGSDGSKVLAVQIPFGYYYGGNIPEDDSDLKEYLKDTIGKLKVKSSTNNVYFPNG
                     FDQAPIRKNLPDILKKANKIDTENEYEISDIENYVDSVRNERNEFTELKGEFVNAKHM
                     RIHKSIFSSRSDLKIMNNKLENYITNVLEPLLTLSYSLGNEYPHLILKDIWKLMFENA
                     AHDSIGSCNSDTTNEDVYTRYKQARDLSINLLDFHMRLISTQIKNNNEEITLTVFNGL
                     PEARNEVVEFDTYIPGQDFVIKNKNGEILNYIIKEKEDITDYILTQTIKLNPSKKIYI
                     PKTVYRAKIALSVKNIPPMGYTQLIVELDTKNKCEIKTSSCKKIENKYYKITANKNGS
                     VEILDKESNKTYKNQAVIEDNGDDGDSYNYSPPRKDIYVSSLDSVSSVEVLKSNVQEE
                     MTLKYSLTIPASLEERTIGKVSVKMPVTMKITLEPKEQIIKFNINIKNDQALSHRVRV
                     LFNTEIASKFSIADQQFGIIQRPTALEKDLALWKRDNYSWQEKPITIEPMQSFVTLED
                     GQKGVALITGCVREYQIVGANLDTIALTLFRSFGYMGRENLLYRPGRASGEKIIATPD
                     AQLLKELNFNFGLYIYNNKFDKSNIANTAKRFLTPIQIYEYADFLNGRLIFVFNDEKQ
                     IYENEYSFMNVNNSNFTVSAIKKEEKGDGIVVRIFNGMKNEDSKGSLNINKNINAAYN
                     AMLDEIYDNTNKLKVNTKTVEVNSLSHCKVQTIVIK"
     gene            complement(7231..9564)
                     /locus_tag="L3M60_001029"
     CDS             complement(7231..9564)
                     /locus_tag="L3M60_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6225718.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDNMYKGSYVLYHNMKEEQEGKMIQPSKLFISKETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNQNFENWESFIKESGKNLKYYLVDKESNFEFFNDNKELGALD
                     TTKNYASKEDSDENKSKNTEMSDEERQKQIKDIKENYRFYIVMNFDKDGKVNVLDSYG
                     VNQKVISQMLLSKAREDLMNTTLGGNPISYKLSPIKNVTLVYAVPKVISKGDGGEYSY
                     GNIYNNDSISSYINNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIILAILQGIVDLYPTSIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIKEYFIKNTVTGMFFVWLIKSGKVVINQIRITDLK
                     EKPNKTIAIILGINLLIIVIMCSFWFFGIILALIYSVVLFKILSDYSKKTIYEYNQLL
                     DVTKKISDGNLEVNMERDLGFFNPIKDELGNIQAGFKKAVDEEVKSQKMKTELISNVS
                     HDLKTPLTSIITYIDLLKDESITDENRKLYIDTLDRKSQRLQHLIEDLFEVSKVNSGD
                     VHLNIMNVDIVSLMKQTLLELDDKLADSSLIVKNNFSNEKIILPLDSQRTFRVFENLI
                     INISKYAMPNSRVYIDILETDNKVSIMLKNMSATEIDFSVDDIMERFVRGDKSRNTEG
                     SGLGLAIAKSFIELQGGKMNISVDGDLFKVTITFNKN"
     gene            complement(9533..10255)
                     /locus_tag="L3M60_001030"
     CDS             complement(9533..10255)
                     /locus_tag="L3M60_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225719.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIIDIMMPKMDGITLTMKLRENHNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVENKEQKVDDDDGVFAIGGLELNENTKEVSVDG
                     KHIKATPIEFKILSLLMRNAGRVFSADEIYERVWNDNAVNTDTVMVHVRNIREKIEVD
                     PKNPKYLKVVWGVGYKIEKIQR"
     gene            complement(10650..11306)
                     /locus_tag="L3M60_001031"
     CDS             complement(10650..11306)
                     /locus_tag="L3M60_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6225720.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVIIKRRDLMEKIADVITKKQQEILVEMEKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            complement(11361..11990)
                     /locus_tag="L3M60_001032"
     CDS             complement(11361..11990)
                     /locus_tag="L3M60_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBN6225721.1"
                     /translation="MVDSIIFDLDGTLWDSTEEVCKVWQDVLSKHKEIDLSVTKDLFR
                     SLMGLSFEEIANRLFPNLSEEERNNLLNECSLKECEYLSENGAKLFDDIENTLKLLSK
                     KYKLFIVSNCQAGYIESFLKAHKLEQYFVDFECPGNTGLHKGENNKLIIERNKLKNPI
                     YIGDTQGDANSAKFAGIPFIYAKYGFGNVDVYDYFIDSFKDLLEHDILK"
     gene            complement(12121..12762)
                     /locus_tag="L3M60_001033"
     CDS             complement(12121..12762)
                     /locus_tag="L3M60_001033"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HBN6225722.1"
                     /translation="MKICIVLNGEIEDYEVTRGIIIKECYDCIICADGGANHTYKMEI
                     MPDYILGDLDSVEEEKINFYKNKGVKFEKFPSKKDETDTELCLFLAKTLKANHIDFFG
                     ALGGRIDHTLANIKLLYYLKEDGIYSRILSDKEEMYIVENEEISLYGNPGDTISVIAI
                     KGDAKGVTLTGLEYPLDNYYMRYSVPIGISNVMLSNSCKIKVEQGCVLVVRNL"
     gene            complement(12771..13442)
                     /locus_tag="L3M60_001034"
     CDS             complement(12771..13442)
                     /locus_tag="L3M60_001034"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBN6225723.1"
                     /translation="MIKLAPSILSADFAKLLEDVRKVESAGCEYLHIDVMDGHFVPNI
                     TLGPLVVKSLKKENINMVFDAHLMIENPDQYIEEFVKAGCDIITVHQEACVHLHRTIQ
                     NIKSHGIKAGVVLNPATPIDTIKHVLPDLDMVLLMSVNPGFGGQSFIPCVLDKIKELK
                     AIIDSQGLNIDIEVDGGISPKNVDKVVQAGANVIVAGSAIFGSDDIQETVNLFRKNAS
                     LEELV"
     gene            complement(13447..14331)
                     /gene="rsgA"
                     /locus_tag="L3M60_001035"
     CDS             complement(13447..14331)
                     /gene="rsgA"
                     /locus_tag="L3M60_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HBN6225724.1"
                     /translation="MLEGKIIKGISGFYYVDTHNGIYECKARGIFRKHKITPLVGDRV
                     KISIVDEDEKKGILEEIDSRDTELIRPPIANVDKALIVFAIKNPKPNLSLLDRFIVLA
                     EKENLETVIILTKADLDDNDTLETVKKIYELSGYKVIPVSNITKLNIEKVKEELKENV
                     VVFAGPSGVGKSSLLNEIDENFKLQTGVVSDKIKRGKHTTRHAELLKLEFGGMVADTP
                     GFSSLALEDIEEVELKDYFIEFDKFNDCKFGSKCIHENEPNCAIKEAVANGEISKERY
                     ESYIQLLNEIRQNNSRRY"
     gene            complement(14429..15166)
                     /locus_tag="L3M60_001036"
     CDS             complement(14429..15166)
                     /locus_tag="L3M60_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN6225725.1"
                     /translation="MKKKTVLITGGARGIGKAMSKAFAKEGYNVLVNFNKSENEAKEL
                     YTRLNEENFSIKLFKADVSNREDVENMVDYCIKEFGGLDVLVNNAGISQDKLFTDITD
                     VDWDNMMNINLKGSFYCSQVALKYMISEKKGNIINISSIWGISGASCEVHYSITKAGI
                     IGMTKALAKEVGPSNIRVNSIAPGVINTDMLSGYNEEDIDILVEETPLMRLGTPEDIA
                     NCAIFLASDKSNFITGQVISPNGGFVI"
     gene            complement(15274..17277)
                     /gene="pknB"
                     /locus_tag="L3M60_001037"
     CDS             complement(15274..17277)
                     /gene="pknB"
                     /locus_tag="L3M60_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HBN6225726.1"
                     /translation="MGDTILGNRYEIIRKIGDGGMAFVYEAKDRLLNRTVALKVLRPE
                     FVDDDEFLTKFKREAEAVASLSHPNIVNVYDVGEDGKVHYIVMEFVDGKNLKEIIQDE
                     GILDEYTALDITKQIAMALSAAHKKGIIHRDIKPHNILISNEGRVVKVADFGIAKAVS
                     NSTMTNIGSIIGSVHYFSPEQAKGKFVTNNADLYSLGIVLYEMLIGKVPFRGDSPISI
                     ALQHINDDIDFTSEEKVRIPQSVRTTIKKLTEKSSADRYQTAEELIEDIEYIEKNIDL
                     DFIKEYDDFATKKIDEKEINKVVNPILAKPAPEKVVKPVEVADLDDDEDYYDDFYEED
                     EEEEEIMRAKKNQRTKSAPSKRTKKKKKKQESPKSRRRLKVIATVLILILCAQVFLAY
                     KFLFSGGFGNKSLTVPNLVNMTLEEAQSAVEKEGLYLSVKSEEYNSEVDENCIISQTP
                     EGGSTNIKKGDTINVVVSKGSSQASVPNVVGLTLSNAKQLIEENNLKVGTVKYEYSSI
                     YKEGTVLNQSPGAGSSRVQEGDEISLYVSKGSEKSNTQTPTVPNKTPTTPNNDTPTEP
                     GSNSGSSGGSSGGSNSGNSGGNSGNNGNNSGGSNSGNSGGNSGGSNSGDNGGNSGGSN
                     SGDNGGSSGGSNSGDNGDSSGGSNSGDNGGTSSGTGANIGKTE"
     gene            complement(17294..18046)
                     /locus_tag="L3M60_001038"
     CDS             complement(17294..18046)
                     /locus_tag="L3M60_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HBN6225727.1"
                     /translation="MVYSCASHIGKIRKNNEDYCEGEVIDTEHGPIGIFAIADGMGGH
                     KKGEVASKLAVENIIDFLKENLLQHDNVKIDYIDDILKQAYNNVNSIVHKKSMEDIEF
                     EGMGTTLVTAIVYNNVLYVANVGDSRCYLLTDEKFDKITIDHSVVEELMMAHVITEEE
                     ARRHPQRNRITRAIGTDDMVVVDIFKKEIKKSDIILLATDGLTGFIDDEDIRDLILDY
                     EYERTSNISEELISMANDVSGKDNVSVIVIKV"
     gene            complement(18066..19097)
                     /gene="rlmN"
                     /locus_tag="L3M60_001039"
     CDS             complement(18066..19097)
                     /gene="rlmN"
                     /locus_tag="L3M60_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HBN6225728.1"
                     /translation="MEKKKIVLKNFTEDELKEFMKTIDEKPFRGSQIFSWIYKGAKTF
                     EDMNNIPKSLRNKLEEISCIGHIDIELKLESKVDNTKKYLFLLDDGNIIETVMMDYDS
                     RVTVCVSNQVGCRMGCNFCASTMDGLIRNLEPWEILDQVIKIQEDTGKRVSNLVLMGS
                     GEPLDNFENTKQFLKIINEKNGLNIGYRHITLSTCGIVPKMYELADLEIAINLALSLH
                     SPYDEERRKIMPVANAYSIKEILDACRYYIKKTNRRVTFEYSLIKGVNDSEKEAKALA
                     KLLKGMLCHVNLIPINKVEEREYEKPDKAFIYKFRDSLEKNNIPATVRMSMGSDISGA
                     CGQLRRKYK"
     gene            complement(19105..20430)
                     /gene="rsmB"
                     /locus_tag="L3M60_001040"
     CDS             complement(19105..20430)
                     /gene="rsmB"
                     /locus_tag="L3M60_001040"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBN6225729.1"
                     /translation="MDAREVGFKVLCDIEKNNNYSNIAINKHFKNLEISDIDRGLATE
                     LIYGVIENKYYLDYIINKLSKIKVKKMSTYVKIFLRMGTYQILFLDSISDYAAVNETV
                     KLSKKYDKKSSGFINAILRNEIRAKETIMDITEEDSVKYLSIKYSYNSWIIKNWIDKF
                     GQEFAEDLLEANSEKPSIYIRTNMLKISREELIEKLSEMGIVCLKVPMVEEAIKVEKM
                     KNIEKNELFKAGLFTIQDISSMIVGKVINPKEDSFVLDVCSAPGGKSTHLATLMNNTG
                     QVVARDIFEHKLKLIKSTVNRLGLKNVCVERFDASEIDENSISKFDYVLADVPCSGLG
                     IIRRKPEIKYKKEEELKDITSIQKKILENASRYVKVGGTLVYSTCTIQDDENINIVAS
                     FVEENHNFEFDSIDTVNVDLDNQDKGYLKIYPNIHGIDGFFIAKLKRIR"
     gene            complement(20492..21193)
                     /locus_tag="L3M60_001041"
     CDS             complement(20492..21193)
                     /locus_tag="L3M60_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HBN6225730.1"
                     /translation="MYPVYGGFWGFDPTMVVLIPAILLTIYAQFKVSSTTNKYLRVNT
                     RRGYTGEQTARRVLDSNGLYDVKIEMVRGHLSDHYDPRRKAVRLSEDVYYGTSITSVA
                     VAAHECGHAIQHAKGYAPLQIRSSLVPVVNFASSISWFLIFLGFIMAGPFLKIGILLF
                     SASVLFQIITLPVEFNASSRAIVQLGNLGIIDESESRQSRRVLSAAALTYVAAALVSI
                     LQLLRLLLIAQRRND"
     gene            complement(21262..22014)
                     /locus_tag="L3M60_001042"
     CDS             complement(21262..22014)
                     /locus_tag="L3M60_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HBN6225731.1"
                     /translation="MTDIKKYLTSVGILILILGLLIGVVNFFIISLTQIFSLIINIII
                     ISLILIILCSTAVTYRVIKGKNINSNIMKINFNIVSGLFPIISFIASSFGMSKSEIRR
                     IYIKLNNEYIYSNKYNFNPEDIIILIPHCIQENSCKLKVTNDIDNCEECGRCNVGELI
                     KLKEKTNVKIFVATGGTLARKIIIDNKPKAVVAVACERDLTSGIQDIKKIPVLGVFNK
                     RPNGPCVNTNVDMIDIEKAIEFLTGKNILACN"
     gene            complement(22024..22953)
                     /locus_tag="L3M60_001043"
     CDS             complement(22024..22953)
                     /locus_tag="L3M60_001043"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBN6225732.1"
                     /translation="MKIVFMGTPDIAVPCLQKIIDEKYEILGVVTQPDKPKGRGKKLG
                     MSPVKELAIENNIPVYQPVKARDKEFIDTIKSLNPDVIVVVAFGQILPKGILEIPKFG
                     CINVHVSLLPKYRGAAPINWVIINGEEKTGVTTMYMDEGLDTGDMILKTEVNLDENIT
                     AGELHDKMMNIGAETLKETLRLIEEGNAPREVQNHEEFSYAPIMNKSLGNIDFSKSAR
                     EIHNLVRGVNPWPSAYTTYNDVIMKVWKTKVLDEKSTKDAGTIIDVSKDGIKVSTIDN
                     VLLIEEIQMPNKKRMLVGEYIKGNTIETGLVLG"
     gene            complement(22968..23408)
                     /gene="def"
                     /locus_tag="L3M60_001044"
     CDS             complement(22968..23408)
                     /gene="def"
                     /locus_tag="L3M60_001044"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBN6225733.1"
                     /translation="MALRQIVQIGEPVLRKKSKKVEKIDEKIIQLLDDMAETMYDADG
                     VGLAAPQVGILKRVVVIDIGEELIELINPEIIETSGEQIDEEGCLSVVGEAGNVRRPN
                     YVKVRALNRNGETIELEGEELLARAFCHELDHLDGILFVDKIEK"
     gene            complement(23426..25951)
                     /gene="priA"
                     /locus_tag="L3M60_001045"
     CDS             complement(23426..25951)
                     /gene="priA"
                     /locus_tag="L3M60_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBN6225734.1"
                     /translation="MNSILNLSRGFIMKKYAKVIVRSNTIYTDNLFTYQIPVFLSEVI
                     RIGHRILVPFGKGNKPTEAFVFQFTDSLDEKIKIKEIIDILDENPIFRKEDLELVYWM
                     KNRYLCTYIECINLIYPKGYKLNNYKVVLLGESLSGLNDIELKEKISTLSDKNREIVE
                     KVIESKGKIKVDKLKYIPNLNSSLYTMNKNGIIKLCWEYKNHKNEKKVCYISLSLESD
                     KIDDYIEQNKINVGSKQKEILSFLKNNENVEINDLLDLLNASKQSINSLSKKKLITLE
                     FKDYYREPKSIYKSVLKSIKLNNEQQKAVDEIKSNMFLDDKKPYLIHGVTGSGKTEVY
                     MEIIEFALNQGLDSIFLVPEIALTPQTIDRLKSRFGDLVGVFHSKLSEGEKHDVYKAV
                     KAGKVRVLIGARSALFAPFNSLGLIIIDECHESSYKSEKNPKFNAIEVARFMALKNNI
                     TLILGSATPSIEEYYRAKYGEYKLINIKSRANDKPLPNIEVVDMKEELDKGNRSIFSL
                     KLQKEIRCAIEENNQVILFLNRRGYANFVSCRKCGYVFQCENCDISLTYHKKSNTGRC
                     HYCGYEKEIPKECPECKSTYVKPFGVGTQKIEEELKYIFPDIKTLRMDKDTTSKKGAL
                     DDILNKFKDKKADVLIGTQMLSKGLDFENVTLVGILSADMILNFPDFKSFETTFQLIT
                     QVSGRAGRADKEGKVVLQTYDTEHYAIKHAIEYDYEGFYEDEIKIRKAFGYSPFNNML
                     SVVVSGEDERLVVKNIKNMHASLIYLLEKRGINDLGFILGPNPCSISKINQNYRWQIL
                     FKDENIEINLLKGIIKYICITKRDLIFDKNISVSIDINPNSVL"
     gene            complement(26190..27389)
                     /gene="coaBC"
                     /locus_tag="L3M60_001046"
     CDS             complement(26190..27389)
                     /gene="coaBC"
                     /locus_tag="L3M60_001046"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBN6225735.1"
                     /translation="MLKDKTVVIGISGGIAAYKACDVVSKLKKLNANIHVIMTKSATE
                     FVNPLTFQSLSQNYVVEDMFEEPKTWEVEHISLAKKADLFLIVPASANVIGKIANGIA
                     DDMLTTTVMATKAQVLIAPAMNTNMYENPIVQKNIQTLKSFGYKFIEPESGRLACGDI
                     GAGKLANPDIIVEEVLANLTKEQDLKGQSMIVTAGPTVESIDPVRYLTNRSSGKMGYS
                     IAREAISRGADVTLISGPTNLSIPQNLKKFVQIESAQDLYEAVVSNLSENKIVIQSAA
                     VADYKPKTYSDKKIKKKNDDLSIELSRNYDIAYEIGKIKEDKILVGFAAETNDLIEHA
                     KGKIKKKNLDFIVANDLTKEGAGFRADTNIVKIIDVEGNITEYPKMKKEEVANVILDK
                     VKALLEK"
     gene            complement(27394..27660)
                     /locus_tag="L3M60_001047"
     CDS             complement(27394..27660)
                     /locus_tag="L3M60_001047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBN6225736.1"
                     /translation="MLKPSINEVLEKIDNRYYLVGTVSKRARKLIDGEEPYVSNKAKE
                     KPVCVATKEVASGKITYRLLTEEEIEIEEARHHAEQHQQISEEE"
     gene            complement(27663..28280)
                     /gene="gmk"
                     /locus_tag="L3M60_001048"
     CDS             complement(27663..28280)
                     /gene="gmk"
                     /locus_tag="L3M60_001048"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBN6225737.1"
                     /translation="MFVKKGLLLVVSGPSGAGKGTICKELLKENDTIKLSVSATTRKP
                     RTGEVDGVNYFFISKEKFEEMIEKGEFLEYAQIYDNFYGTPKSAIMECLEKGQDVLLE
                     IEMQGAKQIKEVCPEGVFIFVLPPSLEELKNRIVGRGTETEAEIEKRFSCAYEEIKMI
                     KDYDYFIFNEDVKTSAKEIEGIISSEKNKVSRYKNIIIEKFKEEL"
     gene            complement(28310..29191)
                     /locus_tag="L3M60_001049"
     CDS             complement(28310..29191)
                     /locus_tag="L3M60_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBN6225738.1"
                     /translation="MAISMTGFGRGEYKDDNYYFLVECKTINHKYSDINIRLPRKISF
                     LEDKVRNLVKNYVKRGRVDLYIKFDLLGKEDVNLNFDEGLASQYIDILKEIKNKFDII
                     DDISVMNVAKFPDIVKIEEKEEDEDLLWSMLNQAVEDALIKLREMRSEEGKKLAEDIA
                     MRCDLLKNHIEEIEKYSSSVVEDYREKLNLRISELLDDPSIIDENRLAQEVAIYADKS
                     SITEEIVRFKSHIGQLKNTIFKDDSIGRKIDFLIQEMNRETNTIGSKSSDINITNLVV
                     EVKSELEKIREQIQNIE"
     gene            complement(29195..30025)
                     /locus_tag="L3M60_001050"
     CDS             complement(29195..30025)
                     /locus_tag="L3M60_001050"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBN6225739.1"
                     /translation="MKFWKLHGIGNDFIAIDGRFDQINPSDYTALAKKVCHRRFSVGA
                     DGLLVVKDSKVADVEMVYYNSDGSRAAMCGNGLRCFCKFVYDNCIVKKESFTVDTLDG
                     IKDIKINLTNREINSIRVNMGKGSFIAKDVPVLTNKERFVQESIKVLDSEFKVTSMLM
                     GVPHTVVIVDELDVEDVCKYGKEIENNKIFPEKTNVNFVKVEDKYNIHVYTWERGCGY
                     TLGCGTGMTASAIVCNLLNMVESSVNVTSQGGTVKIDVGDFSYMTGPAVKICEGILEV
                     "
     gene            complement(30312..31346)
                     /gene="ltaE"
                     /locus_tag="L3M60_001051"
     CDS             complement(30312..31346)
                     /gene="ltaE"
                     /locus_tag="L3M60_001051"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBN6225740.1"
                     /translation="MRFIDLRSDTVTMPTDEMREAMAKAPVGDSIFRDDPTVNKLEEL
                     AAAKVGKEDAIFLPSGTFGNQLALFTHCLRGQEIIIGKGYHIVTHEVGAPAVIAGVQL
                     RTIDEDENGALNPELVEKAIRRDDIHEPQTGLICVENAYSGGTVVGLDNLREIKKIAE
                     KHNLPVHLDGARLFNAALTLGVEAKEIAKCCDSVMFCVSKGLAAPMGSILAGSKDFIA
                     KARRKQKVMGGGMRQVGIVAAAGIIAIEKMTLRLNEDHENAKYLACELNKIDGIEVNN
                     VNPDISMVFFKMSEDIIKEDVLIKEFFERNIKINKMENGEYRFVTHVDITKEDLDYVL
                     KTLKELIGVC"
     gene            31642..33417
                     /locus_tag="L3M60_001052"
     CDS             31642..33417
                     /locus_tag="L3M60_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HBN6225741.1"
                     /translation="MALDGLVIHSIVDELSSKLTGGKIDKIHQPEDDEVIFNIRNNKE
                     NFRLVLSASASNPRVYLTSNYQKENPLKAPMFCMLLRKYIQGGNIVEISQIGFERIIK
                     ISVESLDELKEKTIKNIMIEIMGRHSNIIITHGEENKIIDSIKRVPFSISRVRQVLPG
                     HDYSLPPEQNKLNPLDAISKDLFIKNLEESEESIFKSIYSKFLGISPIIAKEICYRAG
                     INQNTIIKDISDEQFDSLHKVFCNLFNDINSNKYSPCIVIDKKVDRVVDFSCINLTLF
                     SDLSYINKDSMSRILEDFYRTKDIKDRINQRSSDLKKSISVKLDRLYNKLKKQEEELS
                     ESENADIYKIKGELITSYIYMVEKGMESIEVANFYDENCNNVTIELNKNLTPSENAQK
                     YFKKYNKMKHAKVEISHQISLNKEEIDYLENIILSIENCENLAELQDIKEELAKVGYI
                     KTQKKNSKKDTIPSTKPHEFMSSDGFKILVGKNNKQNDYLTLRLADNDDLWMHTKNIP
                     GSHVIIKCAGKEVPDNTVFEGAMLAAFFSKSKLSSQVPVDYTKRKNVKKPSGSKPGMV
                     IYETNSTIYVTPEEETVAKLKVKSE"
BASE COUNT        11161 a         5785 c         3729 g        13027 t
ORIGIN      
        1 caacaaaaaa ttgttctatt tattttttat aaaacttatg taatataaaa ttttatatta
       61 tagacttgat tatggaaaga aaactatcaa aatcattaca attagatata ctatctctaa
      121 tatcattgtc atgaattatc tttaaaagga tatcataaat ggagtttatt tttatataat
      181 catctttatt tatagccaac atcatgatta atttaacagt agaatcttca ctccaatata
      241 ttggttcatt caatatggaa atacaaatct tagtttttat agagcttaat tccataggat
      301 gaggtatagc taaaaaatca tctatattgg tagaagataa actttctctg tagaatacag
      361 attcagtaaa tgatggaatt acaatttcac tttgagttaa tttgttgcac atcaacttta
      421 taagctcatc cttctctttg attttaggat taacaataaa taaattttta tcaaagaaat
      481 tgtccaaaag attactataa atacgagaat tattagtgat tgacttagat atattctcta
      541 tatctttttt taatagtttt aaatctacta atacaatagg aatactatca tgatttaaag
      601 gaattgtcga tattattata tctacatctg acaattcact agctaaaaat tgattaaatg
      661 atagacattg aagtatattt attttatcat gaaaaacttt gtttaattga acctctaata
      721 acctacttga accataccct gaaccacata ctaatactac gtttttacac ttaatattgt
      781 tttgaaaaaa tctctctatt ccagcaccta tatgtaggga tacatatcca atttcatctt
      841 ctgttaaaga gtaaatacta tttttaaaaa ctttttctat agcatttaaa cttatttcaa
      901 atgctaaagg ataattactt tttattgtat ttataagggg attcacctta tttaaattat
      961 aagatttaga attaagaatt gatttaaagt gcagtactaa atcatcaaat aatacactat
     1021 catttctaag gtcaaaagta tattgattgt tgattacttc taaaatttcc tcaacatact
     1081 ctataatttt ggtatctgcc ctattggaaa cgttatccaa aagagaactt ttagttataa
     1141 aatgattata taaataaact ctgtctgcct ttgaaataat tatagtatat ttactctcaa
     1201 tataattaaa tatattttca acagccgtat tcgattgaaa atctgttaaa accacatcta
     1261 aaacattatc taagcagtaa ccacctagta ttctgcttat agtaatagat aaatgaataa
     1321 taaaattttt tagattaaaa tctaagaaat tgatattaaa tttattaatt tcacttagca
     1381 ctatcttctg aagctctatt aaatctacat tattaaataa ttctatttct ctatctgtaa
     1441 ttccaattat ataattttca aaattcctat ttatcatatt attagaaata catcttcgaa
     1501 tatctatttc cttcccttca accttaactc cataatatgg cttagaaact aatattaaat
     1561 tatactttga caaaataggc tttattcttt ttatatcatt tagtattgta aatttactca
     1621 catacaagct attagctaaa tcttctagtt ttatatatgt attgttataa agaatatatt
     1681 ttaaaatata ttgatttctt tctataggag aatctggtat ttcacttgaa tccataatat
     1741 catcagatat taatgctaga taatgttggt ataatgctaa attaagaata tctatataat
     1801 atccttctcc tttttttatt ttaataatag caccattttt ctctaaagtt tcatttatag
     1861 aagtaatatc atttcttata gttctctcac tcacttttaa atgattactt aaagttttac
     1921 aagtaatttt atcatcactt tcatttaata gtttaaataa cgtgagcaat ctcttgtgca
     1981 taaatatccc cccttataga atttcttcta aattatacta tttacactca tataatacaa
     2041 ttactctaat aaaattgtat tatcataatt tatttccact tcgacatgga aattagttat
     2101 tatcataata taaatttcca tgtcgaagtg gaaattagtt atatttaatt taattaggat
     2161 aacgattaca atataaatat aagttaagac attacgaatg gagtggaaaa aatatatggt
     2221 actacaagaa aagaatatat ttatagataa agacttaaaa agcaaagagg aagtattaaa
     2281 ttttattgca gacagtgctt atatactagg tattacaact aataaagatg ctcttttaaa
     2341 agaccttcta aaaagagaaa gcgaatttcc aacaggaata agagatggat ttgcgatacc
     2401 tcatacaaaa agtgcaaatg taaaagaagc ttctatactc tatattaaaa caactaacga
     2461 gttagagtgg aaaactttag atgatagtaa ggttagatat gtatttagtt tgttagctcc
     2521 acatgaaaat gaaggaaata tacatttgaa aactctatct aaattagcca catgcttaat
     2581 ggaagatgat tttataagtg aagttaaaaa tgctagtgat gcaagtcatt tagtaaaata
     2641 tataactaat aaaatgaagg aggaataatt atgaaaatag tagcaataac atcatgtata
     2701 gcaggattgg cacacacacc tatggcagca aaagctttgg aacaagctgc atcaaaaatg
     2761 gggcataaaa tcaaagtaga acaacaaggt gctatgggac gagtaaatac tataacaaaa
     2821 gaagaagcac aagatgcaga ctttatactt atagcatctg accaaactat atctggaatg
     2881 gaaagatttg atggtaaaaa agtaataaaa gttaaaatag gtgtagcatt aaaaaaacca
     2941 gaagctgtct taacaaagtg tatagaaact atttcttaat aaaaagaatc tcactctaat
     3001 ctagaatgag aaaaatcaaa aattaaaaaa ttatctaatg taattataac atattatcaa
     3061 aaaatataac aaacttatat aggaggatta aaaatgagta aaaaaaataa ctctctttta
     3121 agtgaactta aaggacactt aatgacaggt atttcatata tgttgccact tgtaataggc
     3181 tcatctctag ttgtagctat accaaaactt acagctttag caatgggtat aacaagttta
     3241 gatgcttaca aaaatgtatc cggtttttat catattcttt acttaatgga acaagttggt
     3301 tggactggca taggtctatt aaatactgta ttagcaggat ttatagcatt ttcaataggt
     3361 gaaaaaccat ctatgggtgc aggttttatt ggtggactca tagcaagtaa tactaatgca
     3421 ggattcttag gagcagtaac agcaggtttt tttgctggat atatttgcaa atttttaaag
     3481 aaaaaaatta aaatatctgg gtcggcagca ggaatgatgc cacttataat aatgccactt
     3541 ataacagttg gattaacggg gtttttaatg agtgttgtat tagcagggcc tttaggaaac
     3601 ataaatactt cattaaatgc ttgggtagct gaaatgtgtc aaaatggaac aaattcggtg
     3661 gtattggctt taattttagg ttcaatgata ggatttgatt taggaggacc ggtaaataaa
     3721 gcagcttgga tggcaggaaa tgcattattg ttagagggca tctatctacc agcaatattg
     3781 gtaaactgcg caatagttat ccctccacta ggatatggtt tagctacatt cattagaaaa
     3841 tcaagattct ctaagacatt atctgaaaca ggaaaaggca acatagttat gggtataatt
     3901 ggaataacag aaggtgcaat tcctttcact ttaaaaaatc cattaaaatt agttcctgtc
     3961 aatatgatag gatgtgcttt gggtgtagga ttatcagctt tattaggtgt acatgctata
     4021 atgcctccag ttggtggatt atatggattt atatcagtag gttctggatg ggcttactta
     4081 gtaggtgcga tatttggagc tttagtaata gcaatattat caacaatttt agtagacttt
     4141 aatgaagatg aaactgcgga aaatacagaa tttgatgaaa tagagttaga tataatgtaa
     4201 attaaagatt taattttagc tagttgagaa ttgagcgtac tctcaattct caattaaatt
     4261 tagggaggat tttatgaaga agacaaaagt acatgtagtt ccacatagtc attgggatag
     4321 agaatggtat tttacaacat caaggtcaaa aatatatcta atgaaagatt ttcaagatat
     4381 attagacata ttagaagaaa aagaagactt taaatacttt actatggatg cacaagcttc
     4441 tctcttagat gattatttaa aatggagacc tcaggatgaa tatagaataa gagatttagt
     4501 tcaaagaaaa aagttagcta taggaccttg gtatactcaa acagaccaat tggttatatc
     4561 aggagaatct atagttagaa acctttacta tggaatagat agatgcaatg aatttggcga
     4621 acctatgagg gttggatatg taccagattc ttttggacaa tcagctcaaa tgccacaaat
     4681 atatagaggt tttggaatag atagttcatt attttggcgt ggagtttctg atgatatggt
     4741 agatacaaca gaatttatat ggaaaggttc tgatggctca aaggtattag cagttcaaat
     4801 accatttggt tattactatg gaggtaatat accagaagat gattcagatt taaaagaata
     4861 tttaaaagat acaattggaa aacttaaggt taaatcctct actaataacg tatatttccc
     4921 aaatggattt gaccaagcac caataagaaa aaaccttcct gacatattaa aaaaagctaa
     4981 taaaatagat actgaaaatg aatacgaaat aagtgatata gaaaactatg tagattcagt
     5041 aagaaatgaa agaaatgaat ttacagagct taaaggtgaa tttgtaaatg ctaaacatat
     5101 gagaatacac aaatcaatat tctcctcaag gtcagacctt aagataatga ataataaatt
     5161 agaaaattat attacaaatg tacttgaacc attattaaca ttaagttatt cacttggaaa
     5221 tgaataccct cacctaattt taaaagatat atggaaactt atgttcgaaa atgcagctca
     5281 tgacagcata ggttcttgta atagtgacac aacaaatgaa gatgtataca caagatacaa
     5341 acaagccaga gatttgagta taaatttgtt agattttcat atgagactta taagtactca
     5401 aatcaaaaat aataatgaag aaataacatt aacagtattc aatggattac cagaagctag
     5461 aaatgaagtt gtagaatttg atacttatat tccaggtcaa gattttgtga taaagaacaa
     5521 aaatggagaa attcttaatt atataataaa agaaaaagaa gatattacag attatatatt
     5581 aactcaaaca ataaaattaa acccaagtaa aaaaatatat ataccaaaaa ctgtatatag
     5641 ggcaaaaatt gcactttcag taaaaaatat accacccatg ggatatactc agctaattgt
     5701 tgaattagat actaaaaata aatgtgaaat aaaaacaagt agttgtaaaa aaatagaaaa
     5761 caaatactat aaaataacag ccaataaaaa tggttcagta gaaatattag ataaagagtc
     5821 taataagact tataaaaacc aagctgtaat tgaagataat ggagatgatg gagattcata
     5881 caactattcg cctccaagaa aagatattta tgtttcatct ttagattctg tatcttcagt
     5941 agaagtgcta aaatcaaatg ttcaagaaga aatgacactt aaatattcat tgaccatacc
     6001 agcttcacta gaagaaagga ctataggtaa agtttctgtg aaaatgccag taacaatgaa
     6061 aatcacctta gaacctaaag agcaaataat taagtttaat attaatataa aaaatgacca
     6121 agcattatct catagagtaa gagttttatt taatacggaa atagcatcta agttttctat
     6181 agcagatcaa caatttggaa taatacaaag accaacagca ttagaaaaag accttgcatt
     6241 atggaaaaga gacaattact catggcaaga aaagcctata actattgaac caatgcaaag
     6301 ttttgttact ttagaagatg gacaaaaagg agtagcatta ataactggtt gtgtgagaga
     6361 atatcaaata gttggtgcta atttagatac aatagcatta acactattta ggtcatttgg
     6421 atatatggga agagaaaatt tactttatag accaggaaga gcttctggag aaaaaataat
     6481 agcaacacca gatgcacaat tactcaaaga gttaaacttt aactttggtc tttacatcta
     6541 caacaataaa tttgataaat caaatatagc taatactgct aagagattct taacacctat
     6601 acaaatatat gaatatgctg attttttaaa tggtagactt atatttgtat ttaatgatga
     6661 aaaacaaata tatgaaaatg aatatagttt tatgaatgta aataattcaa atttcacagt
     6721 aagtgcaata aagaaagaag aaaaaggaga cggaattgta gttagaatct tcaatggtat
     6781 gaaaaatgag gattctaaag gaagtcttaa catcaataaa aatatcaatg ctgcttataa
     6841 tgcaatgctt gacgaaatct atgataatac taacaagctt aaggtaaata ctaaaactgt
     6901 agaagtaaat tctttgtcac attgtaaagt acaaacaata gtaattaaat aatataaaat
     6961 agcttataca agatttcaaa agaaattact aaccttatgt taatggtaaa tccaaattta
     7021 cactatttaa aatatctata acactaagta acattttata aaataaaaag gtgaatctta
     7081 tatattagga ttcacctttt ttaaataaat caacattcta ttttaaaata agatttacaa
     7141 gttgttttga aataatgttt aaaaactact ttaaaacaat acctataaaa tattatttta
     7201 tggattttat aattattaaa gaagtctatt ctaattttta ttaaaagtaa ttgtaacttt
     7261 aaataaatca ccatccacac ttatattcat cttaccaccc tgtaattcta taaaactttt
     7321 tgctattgct aaaccaagac cagaaccttc tgtgttgcgt gacttatctc ctcttacaaa
     7381 tctttccatt atatcatcta cactaaaatc aatctcagtt gcagacatgt ttttaagcat
     7441 aatacttact ttattgtctg tttctaaaat atcaatatac actcttgaat ttggcattgc
     7501 atatttgctt atatttatta taagattttc aaatactcta aatgtacgct gactatctaa
     7561 tggtaatatt attttttcat tagaaaagtt attctttact attagagatg aatctgcaag
     7621 tttgtcatct aattctaaaa gtgtttgttt cattagagac acaatatcca cattcattat
     7681 gttcaaatga acatctccac tatttacctt acttacttcg aataagtctt ctattagatg
     7741 ttgcaatctt tgtgactttc tatctaatgt atcaatatac aacttacgat tttcatctgt
     7801 tatactttca tcttttaata aatctatata agtgattata gatgtcagag gtgtttttag
     7861 gtcatgtgat acgtttgata taagctccgt tttcatcttt tgactcttaa cctcttcatc
     7921 aacagctttt ttaaatccag cttgaatatt tcctaattca tcttttatag gattaaaaaa
     7981 tcctaaatct ctttccatat ttacttctaa gtttccatct gaaattttct ttgtaacatc
     8041 taaaagttga ttatattcat atattgtctt tttagagtaa tctgacaata tcttaaataa
     8101 tactactgaa tatatcaatg caagtattat tccaaagaac caaaaactac acattataac
     8161 tataattaat aagtttattc ccaaaattat agctatagtt ttattaggct tttcttttaa
     8221 atctgttatt ctaatttgat taataactac ttttcctgat tttatcaacc atacaaaaaa
     8281 cattcctgtg acagtattct ttataaaata ctcttttatt ccaacattta aaatatgttt
     8341 taaaagtatt ataaatacaa atattattga gaatattaca aaccaataag ttagattcat
     8401 taaacttacc aatatactag ccattttagc acttatgtca ttgtttatca gatattctac
     8461 ataatttcca ctcaaagtat tacttattat actagtagga tataaatcta caattccttg
     8521 taaaatagca agaataataa ataatatctc taatggtatt cttgataatg tatcaaatcc
     8581 tagtaattct ttagattttc tatatgggaa tgctaaagct acaatcacaa ctatagctaa
     8641 tataattttt aaaatcattc cagaaatctt atagtaagaa ttattttcta cattatttat
     8701 atatgaactt atactatcat tattataaat atttccataa ctatactctc caccatcccc
     8761 tttacttatt accttaggta ctgcatatac taatgtcacg ttttttattg gagatagctt
     8821 atatgaaatt ggatttccac ctaaagttgt attcattaaa tcttctcttg ctttagacaa
     8881 taacatttga ctaataactt tttgatttac tccataagaa tccaaaacat ttacttttcc
     8941 atctttatca aaattcatta caatgtagaa tctatagttt tcctttatat ctttaatctg
     9001 cttttgtctt tcctcatcac tcatttctgt attttttgat ttattttcat cagaatcttc
     9061 ttttgaagca taattctttg ttgtatcaag tgctcctaat tctttattat cgttaaaaaa
     9121 ttcaaagtta ctttctttat caactaaata atattttaaa ttttttcctg attctttaat
     9181 aaaagactcc caattttcaa agttttgatt aaaatctgca ttaatactat catctgtata
     9241 ataagtctct gagccatcat aagtatcttt ttctttactt acatttttta tatagttcat
     9301 agtttcttta cttataaaaa gttttgaggg ttgtatcatc ttaccttctt gttcttcttt
     9361 catgttatga tataaaacat aacttccttt atacatatta tctacaaaac tttcctgttc
     9421 aaatggattc tttgctccac ctttagcccc tgcttgaata gtagaatatt gactaatcat
     9481 acctaatgat gctgataaca taagtataaa aactataata gtaactatat atttatcttt
     9541 gaattttttc aattttgtat ccaacacccc acaccacctt taaatattta ggattttttg
     9601 ggtctacttc tattttctcc cttatattcc taacatgtac cataactgta tctgtattta
     9661 cagcattatc attccaaact ctttcatata tttcatcagc agaaaaaact cttcctgcat
     9721 ttctcataag taagcttagt attttaaatt ctatgggagt tgcttttata tgcttcccat
     9781 caacactaac ttcttttgta ttttcattta attctaatcc acctattgca aacacgccat
     9841 catcatcatc tactttttgc tctttatttt caaccatatt taaatactta gtgtatcttc
     9901 taagttgtga gtttactcta gccaatagct ctaaaggctt aaaaggtttt gttacataat
     9961 cgtctgcccc tatattaagc cccattattt tatcaacttc ttctgatttt gcagatagca
    10021 ttattactgg gaaattatga ttttctctaa gcttcattgt aagtgttatt ccatccattt
    10081 taggcatcat tatatcaatt attgctaaat gtatttcttg attttctata accttaagtc
    10141 cttctaatcc attgtatcct ttaaaaacat tatatccttg atttaatagg tatatctcta
    10201 tagcatctgc tatttccttt tcatcttcta ccaccaaaat attatatgaa ttcattttaa
    10261 tctcctttat accaattaat ttataaattt tctttacata tatgattata caatatgata
    10321 gaagtttttc cattttttta tatatttata tctttctaat agttattttt atttaatgta
    10381 caccttagag acctccctct agtataaatt tagttttttc attttatata cttaatatta
    10441 aagttaaaaa cttaagatat tacatatact tttctaaaga ttttctaaag atttggagtt
    10501 attcaaaatt aatatgcaca aaaaaggtag tttttattgc attaattaaa tcaatgattt
    10561 gaaacataaa cttaatatca ataaaaccta cctttttatt tttaactatt tatttttaac
    10621 tcattttgaa tcttaaaaat tattgtcttt tattttataa atgtacatat ttcttctact
    10681 ggttttttag atgggctctt caaattatcc tctggcactc caagagccaa cattgcacct
    10741 aaaaaatact ctcctttttc aaatccagtt tctgcttcta aaacagcttc tatttcatct
    10801 gctgcgtagt tttgacttgt caaccaacaa gaaccatacc caagttcaat tgctgataat
    10861 gtaaagtttt caatcgcagc acctaaactc tgcatacctg gatttcttat gaataattta
    10921 tctatagttt ccttaggtgc atctataagc tctaattcat aatatcctga tggattatat
    10981 acctttgtaa aaactaatac taaaactgga gcttttaagt aaaatagtgt aaagttttta
    11041 acaaattttc taaatctatt agccttatct actgatactt tttccatttc tacaagtatt
    11101 tcttgttgct ttttagttat cacatctgct atcttttcca ttaagtcacg acgttttata
    11161 atgacaaaat gccagttttg aatattcttt cctgatggag ctgcacctgc tgcttttatc
    11221 atttttaaaa tatcttcatc tgacacatct tgatttttaa actttcttac actttgtctt
    11281 ttaaaaatag tatcctgtaa ttccattttt aaatcccccc ataacttttt tattatttta
    11341 atataatttt taaactcatc ctattttaat atatcatgtt ctaacaaatc tttaaatgag
    11401 tctataaaat aatcatatac atctacattc ccaaatccat atttagcata tataaaaggg
    11461 atacctgcaa acttagcact atttgcatca ccttgagtat ctccaatata gattgggttt
    11521 tttaatttat ttctttcaat tataagttta ttattttccc ctttatgcaa accagtatta
    11581 cctggacatt caaaatctac aaaatactgt tctagtttat gtgcttttaa aaaagattct
    11641 atatatccag cttgacaatt acttactata aataatttat attttttaga tagtaattta
    11701 agagtatttt ctatatcatc aaataattta gctccatttt cagataaata ttcacattct
    11761 ttcaaagaac attcatttaa taaattattt ctttcttctt cacttaaatt tggaaataaa
    11821 cgatttgcta tttcctcaaa agaaagcccc ataagacttc taaataaatc ttttgtgaca
    11881 cttaaatcta tttctttatg tttacttaaa acatcctgcc aaactttgca tacttcttca
    11941 gttgaatccc acaacgttcc atctaaatca aaaataatac tatctaccac aaaaaatccc
    12001 ccttatgtca aagtttatta tctatttata attatatact aaaaagcaga tacaatcata
    12061 aatatatttt acttgattgt atctgctcta caatataaaa tcaattgtca aaaccaaatt
    12121 ttataaattt ctaactacaa gtacacatcc ttgctctact ttaatcttac aagaattact
    12181 caacataaca tttgatattc caataggtac agagtatctc atataataat tatcgagagg
    12241 atattctaat ccggtaagtg ttacaccctt agcatcacct tttatagcta ttacagaaat
    12301 agtgtctcct ggatttccat acagtgaaat ttcttcattt tcaactatat acatctcttc
    12361 tttatcagaa agtatcctag aatatatacc atcttctttt aaataatata atagctttat
    12421 atttgcaaga gtgtggtcaa tcctacctcc taaagcacca aaaaaatcaa tatgatttgc
    12481 tttaagtgtc ttagctaaaa aaagacaaag ctctgtatca gtctcatctt ttttagatgg
    12541 aaatttttca aatttaacac ctttattttt atagaagttt atcttttctt cttctactga
    12601 atctaagtct cctaatatat aatctggcat tatttccatt ttataagtgt gatttgctcc
    12661 tccatctgca catattatgc agtcataaca ttctttaatt ataatacctc ttgtaacttc
    12721 gtaatcttct atttcaccat ttaaaacaat acagattttc attcttaaat ttaaactaat
    12781 tcttcaagag aagcattctt tctaaataaa ttaactgttt cttgaatgtc atcacttcca
    12841 aatattgcag aacctgctac tattacatta gctcctgctt gtacaacttt atccacattt
    12901 ttagggctta taccaccatc aacttctata tcaatattta atccttgact atctataata
    12961 gcttttaact ctttaatctt atctaaaaca caaggtataa atgattgtcc tccaaatcca
    13021 ggattaacag acattaataa taccatatct aaatcaggta gtacatgttt aatagtatct
    13081 attggtgtag ctggattaag cactacacct gcttttattc catgtgattt tatattttgt
    13141 atagttctat gtagatgaac acaagcctct tgatgaacag ttattatatc acatcctgct
    13201 tttacaaact cttcaatata ctggtctgga ttttctatca taagatgagc atcaaatacc
    13261 atatttatat tttctttctt caaacttttg actacaagtg gtcctaatgt aatgtttggt
    13321 acaaagtgtc catccattac atcgatatga agatattcac atcctgcact ctctaccttt
    13381 cttacatcct ctaataattt tgcaaaatct gctgataata ttgatggtgc taatttaatc
    13441 atgtttctaa tacctcctac tattattttg tcttatttca tttagtagct gtatataact
    13501 ctcatatctt tctttagata tttctccatt tgcaacagct tcttttatag cacaattagg
    13561 ttcattttca tgaatacatt ttgaaccaaa cttacaatca ttaaatttat caaattctat
    13621 aaaataatct ttaagttcaa cttcctctat atcttcaagt gcaagagaac taaagcctgg
    13681 tgtatctgca accattccac caaattccaa ttttaaaagt tcagcatgac gagtagtatg
    13741 ttttcccctt tttattttat cacttaccac tcctgtttga agtttaaagt tttcatctat
    13801 ttcatttagt aaacttgatt ttccaactcc agatggtcct gcaaacacta ctacattttc
    13861 ttttaactct tcttttactt tctctatatt taatttagta atattgctta caggaataac
    13921 tttgtatcca ctaagttcat atattttttt aacagtttct agtgtatcat tatcatctaa
    13981 atctgctttt gtaaggatta ttacagtttc taaattttct ttttctgcca aaactataaa
    14041 tctatctaaa agagataagt ttggtttagg attttttatt gcgaatacta ttagtgcctt
    14101 atctacattt gctataggag gtcttataag ttctgtatct ctactgtcta tttcttctaa
    14161 gatacctttt ttttcatcct catcaacaat gcttattttg actctatcac caaccagagg
    14221 tgttatttta tgttttctaa atattcctct agccttacat tcataaatac cattatgcgt
    14281 atctacataa taaaatccac taatcccctt tataattttt ccttctagca tcaaatgcct
    14341 ccctttataa aatttatata aacacatact gtttatgagt agtatatcgt gttattcttt
    14401 cctattttta tataatacca catttttttt aaataacaaa tcctccgttt ggactaatta
    14461 cttgaccagt tataaaattt gatttgtctg atgccaagaa aatagcacaa tttgctatat
    14521 cttctggagt tcctagcctc attagaggag tttcttccac aagaatatct atatcttcct
    14581 cattatatcc agataacata tcagtattaa tgacacctgg agctatgcta tttactctaa
    14641 tatttgatgg tcctacttct ttagctaatg cttttgtcat tcctattata cctgcttttg
    14701 ttatagaata atgcacttca caagaagcac ctgatatacc ccagattgaa gatatattaa
    14761 ttatatttcc ttttttttct gatatcatgt attttaaagc aacttgtgaa caataaaaac
    14821 ttccttttaa attaatattc atcatattat cccagtctac atcagttata tctgtaaaca
    14881 acttgtcttg acttatacca gcattgttta caagtacatc caatcctcca aattctttta
    14941 tacaataatc taccatattc tctacatctt ctctatttga aacatcagcc ttaaaaagtt
    15001 ttatagaaaa attttcttca tttaatctag tatacaactc tttagcttca ttttctgact
    15061 tattaaaatt tactaataca ttataacctt cttttgcaaa agctttagac atagctttgc
    15121 caattcctct agctcctcct gtaattaaaa ctgttttctt tttcataaat gcttttctcc
    15181 ttatattata accaattcaa tattttatac aaaacaaact gccctttaaa gaaaatattt
    15241 tctataaaag gacagctctt attttattaa tatttattct gttttaccaa tatttgcacc
    15301 agtgcctgat gaagttcctc cattatctcc tgagtttgag cctcctgaac tatctccatt
    15361 gtctcctgag tttgaaccgc ctgagctccc tccattgtct cctgaatttg agcctcctga
    15421 gttccctcca ttgtctcctg aatttgagcc tcctgagttc cctccactat ttcctgagtt
    15481 tgaacctcct gagttgtttc cattatttcc tgagttccct ccactatttc ctgaatttga
    15541 acctcctgag cttcctccac tacttcctga gtttgaacca ggttctgttg gtgtatcatt
    15601 atttggtgtt gttggtgttt tatttggtac tgttggagtc tgtgtatttg atttttctga
    15661 acctttactt acatataaac ttatctcgtc accttcttgc actcttgatg aaccagctcc
    15721 tggactctga tttaaaactg ttccttcttt atatattgaa ctatattcat acttaacagt
    15781 tcccactttt aagttatttt cttcaataag ttgttttgca ttacttagag taagtcccac
    15841 tacatttgga accgatgcct gacttgaacc tttacttaca acaacattta tagtatcacc
    15901 ctttttgata tttgtacttc caccttcagg tgtctgtgat attatacaat tttcatcaac
    15961 ttcactatta tactcttcgc tttttacact taggtataac ccttcttttt caacagcact
    16021 ttgagcttct tctaatgtca tatttacaag attaggaaca gtcaaagatt tattgccaaa
    16081 ccctccagaa aataaaaact tatatgctaa gaatacttga gcacacaata tcaatatcaa
    16141 aacagttgca attactttta atcttcttct agattttggg ctttcctgtt ttttcttctt
    16201 tttcttagtt ctcttacttg gagcactttt agttctctga ttctttttag ctctcattat
    16261 ctcttcctct tcttcatctt cttcataaaa atcatcatag taatcttcat catcatccaa
    16321 atcagctact tcaacaggct taaccacttt ttcaggggct ggttttgcaa gtattggatt
    16381 tacaacttta ttaatttcct tttcatcaat tttcttagtt gcaaaatcat catattcttt
    16441 tataaaatct aaatcaatat ttttttctat atactctata tcttctatca attcttcagc
    16501 agtttgatat ctatctgcac tagatttttc tgtaagcttt tttattgtag ttcttacact
    16561 ttgaggaatc ctaacttttt cttctgatgt aaagtctata tcatcattaa tgtgttgaag
    16621 tgctatagaa attggactgt ctcccctaaa tggaactttt cctatcaaca tttcatataa
    16681 aactatacct agagaataca aatcagcatt attagttaca aatttacctt tagcttgttc
    16741 tggcgaaaaa taatgcactg aacctattat actaccaata ttagtcattg ttgaatttga
    16801 aacagctttg gctataccaa agtcagccac ttttactact cttccttcat tcgatatgag
    16861 gatattatga ggttttatat ctctatgtat aatccccttt ttgtgcgcgg cactaagtgc
    16921 catagctatt tgttttgtta tatctaatgc agtatattca tctaatatac cttcatcttg
    16981 tataatctct tttagatttt ttccatctac aaactccatt acaatgtagt gaacctttcc
    17041 atcttcacca acatcataaa catttactat atttggatgg gaaagacttg ctactgcctc
    17101 tgcctctctt ttaaactttg ttaaaaattc atcatcatct acaaattcag gtctaagtac
    17161 tttaagtgca acagtcctgt ttaataatct gtctttagcc tcatatacaa aggccattcc
    17221 tccatcacca atcttcctga tgatttcata tcgatttcct aaaattgtat ctcccacaat
    17281 atcaccgtcc taattatact tttatcacta taaccgaaac attatcttta ccagaaacat
    17341 cattggccat acttatcaat tcttcactta tattactagt tctctcatat tcataatcta
    17401 atattaaatc tcttatatct tcatcatcaa taaacccagt taatccatct gtcgcaagaa
    17461 gtattatatc actttttttg atttctttct taaaaatatc tacaacaacc atatcatctg
    17521 tcccaattgc tctagttatt ctattcctct gtggatgtct tcttgcctct tcttctgtaa
    17581 tgacatgagc catcataagt tcttcaacca cggaatggtc tatagttatt ttatcaaatt
    17641 tttcatctgt taacaaataa catctgctat ctccaacatt tgccacatat aagacattat
    17701 tatacactat agctgtaact aatgtagttc ccattccttc aaattctata tcttccatag
    17761 attttttgtg cacaattgag tttacattat tataagcctg ttttaatata tcatctatat
    17821 agtctatttt tacattatca tgttgtaata agttttcttt tagaaaatca attatatttt
    17881 ctactgctaa tttactggcg acttcacctt ttttatgtcc acccattcca tcagctatgg
    17941 caaatattcc tattggacca tgttctgtat ctataacttc tccctcacaa tagtcttcat
    18001 tgttttttct tatttttcct atatgggagg cacaactata aaccatacga cttcctcctt
    18061 acaaattact tgtattttct tctcaattgc ccacaagctc cactaatatc agaacccata
    18121 gacatcctaa ctgtggcagg tatattgttt ttctctaaac tatccctaaa tttataaata
    18181 aatgctttat ctggcttttc gtattctctt tcttcaactt tatttattgg aattaaattt
    18241 acatgacata gcataccttt taggagctta gctaatgcct ttgcttcttt ttctgagtca
    18301 tttacccctt ttataagaga atattcaaat gtaacccttc tatttgtctt ttttatataa
    18361 tatctacatg catctaatat ttctttaata gaataagcat ttgctacagg cataattttt
    18421 cttcgttctt catcatatgg tgaatgaagt gataaagcta aatttatagc tatttctaaa
    18481 tctgccaact catacatttt aggtactatc ccacaagttg aaagagttat atgtctatac
    18541 cctatattaa gaccattttt ttcgttaatt atttttaaaa attgttttgt attttcaaag
    18601 ttatcaagtg gttcaccact tcccatcaat acaagatttg ataccctctt accagtatct
    18661 tcttgaattt taataacttg gtctaaaatc tcccatggct ctaggtttct aattaaccca
    18721 tccattgtag atgcacaaaa attacatccc atcctacaac ctacttgatt agagacacag
    18781 acagtaactc tactatcata atccatcatc acagtttcta ttatatttcc atcatctaat
    18841 aaaaacaaat atttttttgt attatctacc ttagattcta actttaattc tatatctata
    18901 tgacctatac atgaaatttc ttctaattta tttctcaaac tttttggtat attattcata
    18961 tcctcaaaag tttttgcacc cttatatatc caagagaata tttggcttcc tctaaagggc
    19021 ttttcatcta tagttttcat aaattcttta agttcatctt cagtaaaatt ttttaatact
    19081 atctttttct tttccattct ctcactacct tattctcttt aattttgcta tgaaaaaacc
    19141 atctatacca tgaatattag gatatatctt taaatatcct ttatcttgat tatctaaatc
    19201 tacatttact gtatcaatcg aatcaaattc aaagttgtga ttttcttcaa caaaagaagc
    19261 aactatattt atattctcat catcttgaat tgtacatgta ctatatacta aagttccacc
    19321 aactttgaca tatctggatg cattttctaa aattttcttt tgtatagaag tgatgtcttt
    19381 taactcttct tctttcttat atttaatctc tggtttacgt cttattatac caagaccaga
    19441 gcaaggaaca tctgctaaaa cataatcaaa tttacttata ctattttcat caatctcaga
    19501 agcatcaaat ctttctacac atacattttt aagtcccaat ctgtttacag tagattttat
    19561 aagttttagt ttatgctcaa aaatatctct agctactact tgaccagtgt tgttcattaa
    19621 agtggctaag tgagtagatt ttcctccagg agcactacat acgtccaaaa caaatgagtc
    19681 ttcttttgga ttaattactt tccctactat catagaactt atatcttgaa ttgtaaataa
    19741 accagcctta aataattcat ttttttctat atttttcatt ttttcgactt taatagcttc
    19801 ttctaccata ggaactttta aacatacaat acccatttca cttaattttt caataagttc
    19861 ttctcgactt attttaagca tattcgttct tatatagata ctaggctttt cactattcgc
    19921 ttccaataaa tcttcagcaa attcttgtcc aaatttatct atccagtttt ttataatcca
    19981 agagttatag gaatacttta tagataggta ttttacacta tcttcctcag ttatgtccat
    20041 tatagtctct ttagctctta tttcatttct aagaatagca tttatgaaac cagaagattt
    20101 tttatcatat ttcttagata atttcacagt ttcattaact gctgcataat cagatatact
    20161 atctaaaaac aaaatctgat atgttcccat tcttaaaaag attttaacat aagttgacat
    20221 tttttttacc tttatttttg aaagtttatt gattatataa tctaaataat atttattttc
    20281 tattactcca taaattaatt ctgttgcaag ccctctatca atgtcactta tttctaaatt
    20341 tttaaaatgc ttatttattg caatatttga ataattgtta tttttttcta tatcacataa
    20401 taccttaaat ccaacttctc ttgcatccat ataaatttct cccttttatt aaacataaaa
    20461 aagggttgtt tctaaacaac ccactttact tttagtcatt tcttctttga gctattagta
    20521 acaatctaag taattgaagt attgaaacca atgctgctgc aacatatgtt aaagctgctg
    20581 ctgaaagcac tcttctactt tgtcttgact cactctcatc tataatacct aaatttccaa
    20641 gttgaactat tgctctactt gaagcattaa attctacagg caatgttatt atttggaata
    20701 acactgaagc cgaaaacaat agtataccaa tttttaaaaa tggaccagcc ataataaatc
    20761 ctagaaatat taaaaaccaa gatatacttg aagcaaaatt cactactggt actaaactac
    20821 ttcttatttg aagaggtgca taacctttag catgttgtat ggcatgacca cactcatgtg
    20881 cagctactgc aacagaagtt atagatgttc cataatatac atcttctgac aaccttacag
    20941 cctttcttct tgggtcataa tggtcactta gatgtcctct aaccatttct atctttacat
    21001 catacaaacc atttgaatca agtactcttc ttgctgtttg ctctccagta tatcctcgac
    21061 gtgtatttac tcttaaatac ttgtttgttg ttgaactcac tttaaattgt gcatatatag
    21121 taagtaatat agctggaatt aatacaacca ttgttgggtc aaatccccaa aaaccgccat
    21181 atacagggta cataaataac actctcctaa aatatatttt tattatacat cctcttaata
    21241 ttcacttaac ttaaaaacag tttaattaca agctaatata ttttttcctg ttaaaaattc
    21301 tatagccttt tcaatgtcta tcatatctac attagtattt acacaaggtc cattaggtct
    21361 tttgttaaat acacctaaaa caggaatttt tttaatatct tgtattccag atgtcaaatc
    21421 tctttcacat gcaacggcta caacagcttt aggtttattg tctattataa tttttctagc
    21481 tagtgttcct cctgtagcta caaatatttt tacattagtt ttttccttta gcttaattaa
    21541 ttctcctacg ttgcatcttc cacattcttc acaattatct atatcattag taacttttaa
    21601 tttacaacta ttctcttgaa tacaatgagg tattaaaatt attatatcct ctggattaaa
    21661 attatattta ttactgtata tatattcatt atttaacttt atatatattc ttctaatctc
    21721 actttttgac ataccaaatg atgatgcaat aaaagatata ataggaaata atccactaac
    21781 aatattaaaa tttattttca ttatatttga gttaatattc tttcctttaa ttactctata
    21841 agtaactgct gttgaacata gtattataag tattagtgat attattataa tatttattat
    21901 taaggaaaat atttgagtta aacttattat aaaaaaatta acaactccta tcaaaagccc
    21961 taatattaaa atcaaaatcc caacacttgt taaatacttc tttatatctg tcatactttt
    22021 cacctaaccc aatactaaac cagtttctat agtattccct tttatatatt caccaaccaa
    22081 cattcttttt ttattcggca tctgaatttc ttcaattaaa agtacattat ctatagtact
    22141 tacttttatt ccatctttgc ttacatcaat tattgtacct gcatcttttg tacttttctc
    22201 atctaacact ttagttttcc aaactttcat tatgacatca ttataggtag tataagcact
    22261 tggccatgga tttacccctc taactaaatt gtgtatttcc ctagcacttt ttgaaaagtc
    22321 tatgttacct aatgatttat tcattattgg ggcatatgaa aattcttcat gattttgtac
    22381 ttctcttgga gcatttcctt cttcaattaa tcttaatgtt tcttttaaag tctctgctcc
    22441 tatattcatc attttatcat gaagttctcc tgctgttata ttttcatcaa ggtttacttc
    22501 tgtctttagt atcatatctc cagtatctag accttcatcc atatacatag ttgtaacacc
    22561 agtcttttct tcaccattta ttattaccca atttataggt gccgcacctc tatattttgg
    22621 aagtaaagaa acatgaacat ttatacatcc aaacttagga atctctaata ttccttttgg
    22681 aagtatctgt ccaaaagcta caactactat tacatctgga tttaaagatt taattgtatc
    22741 tataaattct ttatctctag cctttactgg ttgatataca ggtatattat tttcaattgc
    22801 cagttctttt actggactca tacccagttt tttacctctt ccttttggtt tatctggttg
    22861 agttacaaca cccaatattt catacttctc atctattatt ttttgtaaac atggaactgc
    22921 tatatcagga gttcccataa atactatttt cattaaatca actcctacta cttttctatt
    22981 ttgtcaacaa ataaaattcc atctaagtgg tctagttcat gacaaaatgc tctagctaaa
    23041 agttcttctc cctctaactc tattgtttca ccatttctat ttaaagctct aacctttaca
    23101 taatttggtc ttctaacatt tcccgcttct ccaacaacac ttaagcatcc ttcctcatct
    23161 atttgctccc cagatgtttc tattatttca ggatttataa gttctataag ctcttctcca
    23221 atatctatta ctactactct ttttaagatt ccaacttgtg gtgcagctaa tccaacgcca
    23281 tctgcatcat acatagtttc tgccatatca tctaataatt gtattatctt ttcatcaatt
    23341 ttttcaacct tttttgattt ttttcttaat actggttctc ctatttgaac tatttgtctt
    23401 aaagccatta taattcctcc taaatttata atacactgtt tggattaata tctatagata
    23461 cactgatatt cttatcaaat atcaaatctc gttttgttat acatatatat tttattatac
    23521 cctttaagag attaatttca atattttcgt ctttaaagag tatttgccat ctataatttt
    23581 gatttatttt tgatattgaa catgggtttg gacctaatat aaatcctaaa tcatttatac
    23641 cccttttttc taataaataa attaaagatg catgcatatt ttttatattt tttactacca
    23701 atcgctcatc ttcaccacta acaactacac ttaacatgtt attaaaagga gaatatccaa
    23761 atgcttttct tatttttatc tcatcttcat aaaaaccttc ataatcatat tcaatagcat
    23821 gcttaatagc atagtgttca gtatcataag tttgaagtac tacttttcct tctttatcag
    23881 ctcttccagc tcttccagaa acttgagtta tcaattgaaa tgtagtttca aaacttttaa
    23941 aatctggaaa atttaatatc atgtctgcag acaaaatccc tactaaagtt acattctcaa
    24001 aatccaaccc tttgcttagc atctgtgttc caattagtac atctgctttt ttatctttaa
    24061 atttatttag tatatcatct aaagcacctt tttttgaagt tgtatcttta tccattctca
    24121 aagtttttat atctggaaaa atatatttta attcctcctc gattttttga gttccaactc
    24181 caaatggctt aacataagta cttttacatt caggacactc ttttggaatt tctttctcat
    24241 atccacaata gtggcatctt ccagtattgc ttttcttatg ataagttaaa gatatatcac
    24301 aattttcaca ttgaaacaca tatccacact ttctacaaga tacaaaattt gcataacctc
    24361 ttctatttaa aaacagtatt acttgattat tttcttcaat cgcacaacgt atttcttttt
    24421 gtaatttcaa actaaatatg cttctatttc ctttatctaa ctcctctttc atgtctacta
    24481 cttcaatatt tggaagaggt ttgtcatttg ctctactctt tatatttata agtttgtatt
    24541 ctccgtattt tgccctataa tactcttcaa ttgatggtgt tgctgaacct aaaataagag
    24601 ttatattatt ttttaatgcc ataaatctag caacttctat agcattaaat tttggattct
    24661 tctcagactt atatgatgac tcatgacatt catcaattat tattaagcct aaagaattaa
    24721 aaggtgcaaa taaagccgac ctagctccaa ttaacactct tactttacct gccttgacag
    24781 ccttataaac atcatgtttt tctccttcag atagtttgct gtgaaacaca ccaactaaat
    24841 ctccaaacct acttttaagc ctatctattg tttgaggagt taatgctatc tcaggtacta
    24901 agaatatact gtctaagcct tgatttagag caaattctat tatttccatg taaacttcag
    24961 tttttccact tccagttact ccatgaatta agtatggttt tttatcatct aaaaacatat
    25021 tcgactttat ttcatcaact gctttttgtt gctcattgtt taattttata ctttttaaca
    25081 cggatttgta aatacttttt ggctctctat aataatcctt aaattctaag gttattagtt
    25141 ttttcttgct tagagaattt atactttgtt ttgaagcatt taataaatcc aataaatcat
    25201 ttatctctac attttcatta tttttaagaa aacttaatat ctctttttgt ttacttccta
    25261 catttatttt attttgctct atataatcat caatcttatc actttccaaa ctcaaagata
    25321 tataacatac tttcttttca tttttgtgat ttttatattc ccaacataat tttataattc
    25381 catttttatt cattgtgtac aaagaactat ttaagtttgg tatatatttt aacttatcaa
    25441 ccttaatttt acctttactc tcaatcactt tttctactat ttctctattt ttatcactta
    25501 aagttgatat tttttccttc aattcaatat catttaatcc acttaaactt tcacctaata
    25561 ggactacttt ataattattt agtttgtatc cctttggata tatcaaattt atacattcta
    25621 tataagtgca caaatatcta tttttcatcc aataaaccaa ctctaaatct tcttttctaa
    25681 atattggatt ttcatctaat atatcaataa tttcttttat ttttattttt tcatctaagc
    25741 tatctgtaaa ttgaaataca aaggcttctg ttggtttatt accttttcca aatggtacta
    25801 atattctgtg tccgattctt atgacctcag ataaaaatac aggaatttgg tatgtaaata
    25861 aattatctgt gtatatagta ttacttctaa ctattacctt tgcatatttt ttcattataa
    25921 aacctctact taaatttaat atactgttca tattttaata tactttacaa gacattaaaa
    25981 tttcataatt tataaacaat tccaagttat taatattaac tttattttaa taattaacta
    26041 ttaattatat tttatacttt atgacaaaat aaatgctgaa aactacaatt taactgacat
    26101 ttgcgagttt ttactcgcta aaattaaatt gcagctttca gccactttag ctgtgataaa
    26161 atacacaaat atcatattta aaaaaggtct tatttctcaa gcaacgcttt aactttatct
    26221 aaaataacat tggctacttc ttcttttttc attttaggat actcagtaat atttccttct
    26281 acatctatta tttttacaat attagtatca gctctaaatc cagcaccttc tttagttaaa
    26341 tcatttgcca caataaaatc taggtttttc ttttttattt ttcctttggc atgttctatc
    26401 aggtcattag tttcagctgc aaatcccact agtatcttat cttcttttat ttttcctatt
    26461 tcataagcaa tatcataatt tctacttaac tctattgata aatcatcatt cttcttttta
    26521 atttttttat cagaataagt ttttggctta taatctgcaa ctgctgcact ttgaatcact
    26581 attttatttt cacttaaatt tgatactact gcttcgtaca aatcttgtgc tgactctatt
    26641 tgaacaaatt ttttaagatt ttgtgggata gaaagatttg taggacctga tattagtgtt
    26701 acatcagcac ctctagatat agcttctctt gctatagaat atcccatttt accagaagac
    26761 ctattagtta aatatcttac agggtctata ctctctacag ttggaccagc tgtaactatc
    26821 atactttgac cttttaaatc ttgttcttta gtaagattag ctaaaacttc ctccactatt
    26881 atatctggat tggccagttt acctgctcca atatctccac atgcaagtct tccgctttct
    26941 ggttcaataa atttatatcc aaagctcttt aaagtttgta tatttttttg aacaattgga
    27001 ttttcataca tattagtatt catagctggt gctataagaa cctgtgcttt tgttgccata
    27061 acagtagttg tcaacatatc gtcagctata ccatttgcaa tcttacctat aacatttgca
    27121 cttgctggca ctattaaaaa taaatctgct ttttttgcta atgatatatg ttcaacttcc
    27181 caagtttttg gttcttcaaa catatcttct actacataat tttgactcag tgattgaaat
    27241 gtaagtgggt taacaaattc tgttgctgat tttgtcatta taacatggat atttgcattt
    27301 agttttttta acttacttac aacatcacaa gccttatatg cagctattcc gccgcttatg
    27361 ccaattacaa cagttttatc ttttagcatg accttactcc tcttctgaaa tttgttggtg
    27421 ttgctcagca tggtgtctag cttcttctat ctctatttcc tcttctgtca ataatctata
    27481 tgttattttt ccacttgcaa cttctttagt tgctacacat acaggcttct ccttagcttt
    27541 atttgaaaca tatggctctt ctccatcaat taattttctt gctctctttg aaacagtccc
    27601 tactaaatag tatctgttgt ctattttttc tagtacctca ttgatagatg gttttaacat
    27661 aattataatt cctccttgaa tttctctatt ataatatttt tatatctgct aactttattc
    27721 ttttcagatg atattatacc ttctatttct ttagcagatg tttttacatc ttcattaaat
    27781 ataaaataat cataatcctt aatcatttta atttcttcat atgcacagct aaatctcttc
    27841 tctatttcgg cttctgtttc tgtacccctt ccaacaattc tattttttag ttcttcaagt
    27901 gatggaggta gtacaaatat aaatactcct tctggacaaa cttcttttat ttgttttgct
    27961 ccttgcattt caatctccag taatacatct tgtccttttt ctaaacattc cattattgct
    28021 gattttggag ttccataaaa attatcataa atttgagcat actccaaaaa ctcacctttt
    28081 tctatcatct cttcaaactt ttccttactt atgaagaaat aatttactcc atcaacttct
    28141 ccagttctag gttttcttgt agtagctgat acagaaagtt ttattgtatc attttcttta
    28201 agtagttctt tgcaaatagt accttttcct gcacctgatg gtcctgatac aactagcaat
    28261 aatccttttt tcacaaacat ttaagcattc cccttctaat attgtattct tactctatat
    28321 tttgtatttg ttcccttatt ttttctaatt cgctttttac ttctactact aaattagtta
    28381 tatttatatc agacgacttc gaacctatag tgttagtctc tctattcatt tcttgtatta
    28441 agaaatctat ttttctacct atagagtcgt ctttaaatat agtattttta agttgcccta
    28501 tatgactttt aaatctaact atttcttctg ttatactgct tttatctgca tatattgcaa
    28561 cttcttgcgc taatctattc tcatctatta tacttgggtc atccagtaat tcacttattc
    28621 tcaaattcaa tttttctcta taatcttcca caacactact tgaatatttt tcaatttctt
    28681 ctatatgatt ttttaataaa tcacatctca tggcaatatc ttcagctaac tttttacctt
    28741 cttcacttct catttctctt aatttaatta atgcatcttc aactgcttga ttcaacattg
    28801 accataataa atcttcatct tcttcctttt cttctatttt cacaatatca ggaaactttg
    28861 caacattcat aacactaata tcatctataa tgtcaaattt gttttttatt tcttttagta
    28921 tgtctatata ctgagatgct aatccctcat caaaatttaa gtttacatct tctttgccta
    28981 ataagtcaaa ctttatgtat aaatctactc ttcctctttt tacataattt ttcactaaat
    29041 ttctaacttt atcctctaaa aatgaaattt ttcttggaag acgtatgttt atatctgaat
    29101 acttatgatt tatcgtttta cactctacta aaaagtaata attatcatcc ttgtattctc
    29161 ctcttccaaa accagtcata cttatagcca ttaattaaac ctccaaaata ccttcacata
    29221 tttttactgc tggacctgtc atataagaga aatctcctac atctatttta actgttccac
    29281 cttgagacgt tacatttaca ctactttcta ccatattaag taaattgcac actattgctg
    29341 atgcagtcat accagtacca caacctagag tatacccaca acctctttcc caagtataaa
    29401 catgtatatt atacttatct tctaccttta caaaatttac attcgttttt tcaggaaata
    29461 ttttattatt ttcaatctct tttccatatt tacaaacatc ctctacatct aactcatcta
    29521 ctataactac tgtatgagga acccccatca acatagaagt aactttaaac tcactatcta
    29581 aaacctttat actttcttgc acaaaacgtt ctttatttgt aagtacagga acatccttag
    29641 ctataaaact tccttttccc atattgactc ttatagagtt tatttctcta ttggtaaggt
    29701 ttatttttat atcttttata ccatctaatg tatctactgt aaaactttct tttttgacta
    29761 tacaattatc ataaacaaat ttacaaaaac atctaagacc atttccacac atagctgctc
    29821 ttgaaccatc agaattataa tataccattt ctacatctgc aacttttgaa tctttcacaa
    29881 ctaacaatcc atccgctcct acagaaaatc ttctatggca tacttttttt gctaaagcag
    29941 tataatcaga tgggtttatc tggtcgaatc ttccatctat tgctataaaa tcatttccta
    30001 ttccatgtag tttccaaaat ttcataagta tcgctccttc gtaaattaac acttaaacta
    30061 ttataacata aaaaaattcc cataaactta taatttatgg gaaaaatttt gtaatgacaa
    30121 ctataatttg aggatgtgag ggagagttgc cattacaatt agggtaatta tattatgttt
    30181 ttaagctagt tctttctatg tttatataat aatatttcta tatgaatttt atgtatgttt
    30241 tatttaaact gtctatgaat tttttaatta aacatctgat aaccagctat aattcttttg
    30301 gtagatatct attaacatac acctattaat tctttaagtg tttttaaaac atagtctaaa
    30361 tcttccttag taatatctac atgagttaca aatctatatt caccattttc cattttatta
    30421 atttttatat ttctttcaaa aaattctttt attaaaacat cctcttttat aatatcttca
    30481 ctcatcttaa aaaataccat acttatatct ggattaacat tgttcacttc aatgccatca
    30541 attttattta gttcacaagc taaatatttt gcattttcat ggtcttcatt caatcttaaa
    30601 gtcatttttt caatggcaat tataccagct gctgcaacta tacctacttg tctcattcct
    30661 ccacccataa ctttttgctt tcttctagct tttgctataa agtctttact tccagcaagt
    30721 atagaaccca ttggtgcagc taaacctttt gacacacaga acataacact atcacaacac
    30781 tttgctattt ctttagcttc tacacccaaa gttaatgctg cattaaataa tcttgctcca
    30841 tctagatgta ctggcaagtt atgtttctca gctatttttt taatttctct taaattatca
    30901 agacccacaa cagttccacc tgaatatgca ttttctacac atataagccc tgtctgtggt
    30961 tcatgtatgt catctcttct gattgccttt tcaacaagtt caggattaag agctccattt
    31021 tcgtcttcat ctatagttct aagctgaact ccagctatta cagcaggtgc cccaacctca
    31081 tgagttacaa tgtgatatcc tttaccaata attatttctt gtcctcttag acaatgtgtg
    31141 aatagagcta attgattacc aaaagttcca cttggaagaa aaatagcatc ctcttttcca
    31201 actttagctg ctgctaattc ttcaagttta tttacagtag ggtcatctct aaagattgaa
    31261 tctccaacag gagcctttgc catagcttct ctcatttcat cagtaggcat agtaacagta
    31321 tcacttctta aatcgataaa tctcattttt tcaaccttct ttcatattta tatactatag
    31381 aaccttaaaa ttgatttttt ctgtaaagtt catatacttt catagcttat actttacaca
    31441 ataattatac tatatttact ttatatttaa tctactttaa attattaatt ttaaaaataa
    31501 agtttccttt ctaataagta taaataatta tacaatttaa atattaatct ctattgttta
    31561 aattccctct agtcacaaag cgtatattat gtgtataatt aatttactga ctcattttgg
    31621 tcaaagaaag gggtgctata catggcatta gatggattag ttatacattc aatagtcgat
    31681 gaattatcat caaaacttac aggtggaaaa atagataaga tacatcagcc tgaagatgat
    31741 gaggtaattt ttaatataag aaataataaa gaaaatttta gattggtttt gagtgcaagt
    31801 gcctcaaatc ctagagttta tcttacaagc aattatcaaa aagaaaaccc tctaaaagca
    31861 cctatgtttt gtatgttact tagaaaatac atacaaggtg gaaatatagt agaaatttct
    31921 caaataggtt ttgaaagaat aataaaaata agtgttgaat ctttagatga actaaaggaa
    31981 aagacaatta aaaatattat gattgaaatt atgggaagac atagcaacat aataataact
    32041 catggtgaag aaaataaaat tatagattct ataaaaagag tcccttttag tataagtaga
    32101 gttcgtcaag ttttacctgg tcatgattat tcattacctc cagagcaaaa caaactaaac
    32161 cctcttgatg ctatatctaa ggatttgttc attaaaaacc tagaggaatc agaagaatct
    32221 atctttaagt ctatatattc taaatttcta ggtataagcc ctattatagc taaagaaatt
    32281 tgctacagag caggtataaa tcaaaacacc atcataaaag atatatctga tgagcaattt
    32341 gattctttac acaaagtatt ttgtaattta tttaatgata taaattctaa taaatactca
    32401 ccttgcattg ttatagataa aaaggtggat agggtagttg atttcagctg tataaacctt
    32461 acattattta gtgatttatc atatataaat aaggatagta tgtctcgtat acttgaagat
    32521 ttctatagaa ctaaagatat taaagataga attaatcaac gttcatctga tttaaaaaag
    32581 agcatatctg ttaaacttga tagactttat aataaactta aaaaacaaga agaagaatta
    32641 tcagaatctg aaaatgcaga tatttataag ataaaaggag aattgattac ttcatacatc
    32701 tatatggtag aaaaaggtat ggaaagtatt gaagttgcta acttctatga tgaaaattgt
    32761 aataatgtaa caatagaatt aaataaaaac ttgacaccat ctgaaaatgc tcaaaaatac
    32821 tttaaaaagt ataataaaat gaaacatgct aaagtcgaga tatctcatca aatttcctta
    32881 aataaggaag aaatagatta cttagaaaat ataattttaa gtattgaaaa ttgtgaaaat
    32941 ttagctgaat tacaagatat aaaagaggaa ctcgctaaag ttggatatat caagactcaa
    33001 aagaaaaaca gtaaaaagga tactattcca tctacaaagc ctcatgaatt tatgtcttct
    33061 gatggattta aaatattggt tggtaaaaac aacaagcaaa atgactatct tacactaaga
    33121 cttgctgata acgacgactt atggatgcat acaaagaata ttcctggttc tcatgtaata
    33181 ataaagtgtg ctggtaaaga agtccctgat aatactgtat ttgaaggagc catgctagcc
    33241 gcattcttta gtaagtcaaa actttcttct caggtgcctg tagattatac taaaagaaaa
    33301 aatgtaaaaa aacctagtgg ttctaaacca ggtatggtta tttatgagac taacagtact
    33361 atttatgtta ctcctgaaga agaaacagta gctaaactta aggttaaatc tgaataaatt
    33421 ctgcactaaa tataaaataa gtgttagaca agcacacatt atcatttaaa atgaaaatag
    33481 tggtcttatt atgaagtaca ataataaggc cactttctaa tcaacacact tttatattct
    33541 aataactata cttatattcc taataagtat ttttagtctt cttatataga tataattaaa
    33601 aagttaatac taaaagctat tttttaattt attgatttgt tttagtagtt cattgtattc
    33661 tgattcataa agttcttttt ttagaccatc cttttctata ga
//
LOCUS       DAFNNB010000025        33386 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0025, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000025 DAFNNB010000000
VERSION     DAFNNB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 33386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33386
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0025"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..214
                     /locus_tag="L3M60_001053"
     CDS             <1..214
                     /locus_tag="L3M60_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBN6225742.1"
                     /translation="DAEKMYLNVNPDFTKDYVLGNIRIHIKSRIKDLFNIIIMINKIM
                     NKNKGNKEGDSNESNRFNTESYGNNS"
     gene            168..596
                     /gene="ytfJ"
                     /locus_tag="L3M60_001054"
     CDS             168..596
                     /gene="ytfJ"
                     /locus_tag="L3M60_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HBN6225743.1"
                     /translation="MRATGSIQSLMETTLETIKGSIDANTIIGDPIKTDTTVVVPISK
                     VTIGFGIGGGEYSKGYDDKDREIELKNEKSDTNFAGGSAGAISVQPVAFVVVESGETR
                     IMSLDSNINLVDNILSITPRVLEKIQNISQSNKNQDKNNM"
     gene            834..1274
                     /locus_tag="L3M60_001055"
     CDS             834..1274
                     /locus_tag="L3M60_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6225744.1"
                     /translation="MSDKELSILLLESMNNFHNLIKIINNERYKKDKVLTERQFFALV
                     KIRKHDKIELKNLSRDLHVSTSSLCILLNKLVEQEYVYREEDSRDRRNTFYGITKNGE
                     KILDNEILKFVSIISDKMDCLDIDNKDKLFTSLEESKNIIEQLF"
     gene            1290..2741
                     /locus_tag="L3M60_001056"
     CDS             1290..2741
                     /locus_tag="L3M60_001056"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBN6225745.1"
                     /translation="MNIKCSKEILKSLENAITVVALFEGEDVSTTFDKINMISRNFNS
                     KENSIDTVTLVENNNLVNVCLVGFGKRELLNREKIRIIGGNLCKKLKNILKNEVYKSS
                     DVNVLNLNLNSDEIGSFAEGILLGNYKFDRYKTKSKNFERKEVDTITIFTEKDVECQI
                     KKAKILANSTIIARNLVNEPSNVIYPKTLAIETVKLGAEFGFNVDVYEEEKIKALGMD
                     AFFAVSRGSINKPRFIVMRYFGDKESTDILGLVGKGLTYDTGGYSLKSNASMLDMKTD
                     MAGAASVIGAMCAISQSKLKKNVIAVVAACENALSGGSYKPGDIINSMANKTIEVLNT
                     DAEGRLTLADAIYYIINNEKVTKVVDVATLTGAALTLLGNVATPIVTNNDDFYCELEK
                     AATLSGERVWKMPIYDEFKDMIKGEEADLKNTGGKNAGCITAGAFIGEFVGNTPWIHM
                     DIAGTSTSSKSIGYKVKGATGEPVRTLYYLAEI"
     gene            complement(2833..3501)
                     /locus_tag="L3M60_001057"
     CDS             complement(2833..3501)
                     /locus_tag="L3M60_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwaF family protein"
                     /protein_id="HBN6225746.1"
                     /translation="MFKIFLFSSEQFVSLFIFGLFLYYCPKLTKNILPYSYTVEKIIC
                     TLLVIIMALEQLLLISSGNYSTLNSLPIGINYICIYLCIAILIFKQYHLFNIFFSWSL
                     VCSVGELIFSTNLGYEFPSLIYFIFIFSKCLIIYADIYMVDVRKFKVNRYALRDNLAI
                     CFIYFSFIFLLNTLTNSQYYYGFLSHSTTAIFTFIFVTSIMYIPALLFNRDTFILEKK
                     KKSK"
     gene            3770..3910
                     /locus_tag="L3M60_001058"
     CDS             3770..3910
                     /locus_tag="L3M60_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225747.1"
                     /translation="MGIIESASKLAEMVHLLAVEKGITDIEAWDEAVKEYSKIYEERR
                     NE"
     gene            complement(4085..4405)
                     /locus_tag="L3M60_001059"
     CDS             complement(4085..4405)
                     /locus_tag="L3M60_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HBN6225748.1"
                     /translation="MKLKTISLPELNNLDPTLESTFIKMGEEQGELAECIGKFRNLSG
                     ENNDLDEVDIIKKTAKELMDVAQTCVTMMFKLEEQYGINLDEIRKEHIKKLEKRGYIK
                     NIDK"
     gene            complement(4525..5262)
                     /locus_tag="L3M60_001060"
     CDS             complement(4525..5262)
                     /locus_tag="L3M60_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBN6225749.1"
                     /translation="MDANSLKDLIANHNNIVFFGGAGVSTESNIPDFRSSTGLFSQKL
                     NKQFTAEQLVSHTFFVRYPEEFFEFYKDKLIYPNAKPNNAHIALAKLEEMGKLKAVIT
                     QNIDGLHQMAGSKNVLELHGSVHRNYCTKCGKFFDLESMLNLGGNIPYCDNCGSIVKP
                     DVVLYEEALDSDIITKTISAISNADLLIIGGTSLAVYPAASFIDYYKGNYIALINKAN
                     TVYDKSASLVINKPIGEVLYEAVLRQI"
     gene            5476..7296
                     /gene="acd"
                     /locus_tag="L3M60_001061"
     CDS             5476..7296
                     /gene="acd"
                     /locus_tag="L3M60_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HBN6225750.1"
                     /translation="MKKKAALATLAMLPLSVVNAHADGDIGIVTINYLNVRNEPTAES
                     SIAFVAKKDDKVLIKDSSNGWYKIKAESGQEGWASSKYIAKLNGDSLRTSTNKEKQVT
                     SNSLNMRNGAGTSYRVITVLKKGQKVEVISESNGWSKIKYDGRLGYVSSSYLGDVSNS
                     TNKSKTKQVNTTSLNVRSGPNTSYGLLGKLSKGSKVEVISESNGWSKIKYNGKDAYVS
                     SMYLSDVSQSNSDDSSQSNDKKNTDKFVNTASLNVRSGPGSTYSKLGKVYKGSKVTVL
                     SESSGWAKINFNNKEAFVVGNYLSTSADASNNNSNSNSDSSSSNNGNNSSSGQVNGMS
                     GISGAKIDYKSLNYTLASHISKQVEKAASGGNVIAPSNRKSTPSPEFSTFSAQRTSSF
                     VNASSSDIEYYLNPKNFINTTKGMMQFLKINSYRGGISESSLNSYLNGLSSSVFKNQG
                     ATFINAAKKYNIDVVYLVSHAMWETAYGKSTLAQGQTLTSYKGQPLSKPVKVYNFFGI
                     GAIDKSANVSGAEAAYSNGWTSVEATIDGSAKWISQNYINSSKYNQNTIYKMKWNYDY
                     TWHQYATDVNWANGISGIMENLIGLYGGGSSLVFEVPQYK"
     gene            7509..11807
                     /locus_tag="L3M60_001062"
     CDS             7509..11807
                     /locus_tag="L3M60_001062"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HBN6225751.1"
                     /translation="MESIKEYLDKLEINNSGLGKQLKEVYINRVVYFKEDKIVYFYLT
                     SKDIVSHELLDKFKEELMYKLDYFKDMRMKIRFTGLERKSNKDVIKKYWNNILYILKY
                     LCPSIAGWYKQVEFLCLEEELKIKLPKGIFYERLMKKNVTYVLKTVLSEELGLDLNIT
                     IEKAVDEKINKERLIRINDREMEEKIRALEIGKVNSCEESEEEGYVIKSEVDEELIYG
                     DNANAMIENIVELNASSGTVAVVGDIFDVDTKELKNGKILMIASITDYTSSISCKLFL
                     TDTNKDGVLENVKKGAYLKIKGDIVYDTYQREISMMISGIRKETRKEREDKSEEKRVE
                     LHAHTQMSSMDAICSVKKLVERAAKWGHPAIAITDHGVVQGFPDAMNAGKSNNIKILY
                     GVEGYLVEEDSLIIEDANDKELSQTFVVFDIETTGFSNTNDKITEIGAVKIENFKIVD
                     RFSELINPEKDISYKIQELTGITNELIKDKPTIEEVLPKFMEFVGDSVLVAHNAEFDT
                     GFISQKCREQGLLYNNKKVDTLMLARVMLPNLKRHRLNVVAKELGIPLLNHHRAVDDA
                     EATALIFNKFLQMLTEKGAKTLSDVNNILGKIDYTKLSTNHITLIAKNSVGIKNLYKI
                     ISDAHVNHFFRAPRILRSVLNEYKEGLIIGSACEAGVVFQAVKKNVSDEEMKKIIDLY
                     DYIEVMPIDNNRFMIDKGEVKDEEELRELNRKLIDTAKKFNKIPVATGDVHFLDKHEA
                     ILRKVLKYSQGFKVDEEETYLHFRTTDEMLEEFSYLGEELAYEVVVENSNLIADMVED
                     IKPIPDDTYPPIIEGSDVELREMCYKKAKRIYGDPIPEIVQKRLDRELNSIISNGYAV
                     MYIIAQKLVAKSLSDGYLVGSRGSVGSSFAATMSDITEVNPLPAHYICENEDCKYSFF
                     YEIGEWGSGVDLPDKDCPKCGRKLAKNGHDIPFEVFLGFEGDKEPDIDLNFSGNYQPT
                     IHKYTEELFGEGYVYRAGTIGTVAEKTAFGYARKYVEENNISVPNAEVLRLSNGCTGV
                     KRTSGQHPGGVMVIPDYKDVYDFTPIQYPANDTSCGVITTHFDYHSISGRILKLDILG
                     HDGPTIIRMLEDMTGINITEIPLDDKETMSLFTSTEALGVTPEEINCPIGCLAVPEFG
                     TKFVRQMLLDTKPTTFAELVRISGLSHGTDVWVNNAQDLVREDIVGLKDVISTRDDIM
                     NYLIFKGLPPKMSFTIMESVRKGKGLKPEHIAEMKKNNVPEWYIGSCKKIKYMFPKAH
                     AVAYVMTSFRIAYCKVHYPEAFYATYFTTKVEDFDADLIVKGLDAIKSKINEIESLGN
                     DATTKEKGMLTVLEVALEMYARGIKLLPVDIYKSDATEFIIVGEKTLLPPMAAIQGLG
                     GNAAINIQTERKNGEFISKEDLRKRTKISKTVIETLTNHGSLENMSERNQLSLF"
     gene            12011..12472
                     /locus_tag="L3M60_001063"
     CDS             12011..12472
                     /locus_tag="L3M60_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBN6225752.1"
                     /translation="MKKNLEATIEEIVTKITDEHGFEMVDVEYVKEAGEYYLRVYIDK
                     EEGISLNECELVSRELSPILDEKDPIKENYFLEVSSPGLDRALKKDRDFVRYQGRDVD
                     LKLYKPLNGCKQFEGELVGLTEDNNIKIIVNGKEIEFNRKDVAIVRLAIKF"
     gene            12502..13656
                     /gene="nusA"
                     /locus_tag="L3M60_001064"
     CDS             12502..13656
                     /gene="nusA"
                     /locus_tag="L3M60_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBN6225753.1"
                     /translation="MNHEFMEALDELVVDRGIDKEILIDTIEQALLTAYKKNFGSAQN
                     VRVEFDRDKGDIKVFSQRVVVDESDLYDTFLEIELADAREINPNYELGDIIEHEVTPK
                     NFGRIAAQTAKQVVVQRIREAEREIVYNEFIEKENEIVTGEVARANKNVVYVNLGRIE
                     GIMTQSEQIPFENYKAGQKIKFYILEVKKTNKGPQIVVSRSHPGLVKRLFEEEVPEIY
                     EGIVQIKSVAREAGSRTKMAVRSIDEKIDPIGACVGPKGLRVKNIVDELGDEKIDIIK
                     YSEDPAEFISAALSPSKVVRVDIDEDEKSALVIVPDYQLSLAIGKEGQNARLAAKLTN
                     WKIDIKSESQAEQEMLNLENSKKIEAEDVEAEFIEQEELDTSDDNFSDEE"
     gene            13663..13944
                     /locus_tag="L3M60_001065"
     CDS             13663..13944
                     /locus_tag="L3M60_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HBN6225754.1"
                     /translation="MILKKVKKVPQRKCIACQDRDSKKELIRIVKNKEGKIFLDPTGR
                     ANGRGAYVCKSSECLKKAIKSKALNKAFKIDVPDEVYDNLLMELEEYEK"
     gene            13934..14245
                     /locus_tag="L3M60_001066"
     CDS             13934..14245
                     /locus_tag="L3M60_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HBN6225755.1"
                     /translation="MKNNKEKVLSFLGLATRAGKIVSGDDSTLLDLKKGKVNLILIAE
                     DASNNTKKLFKDKSTFRNIPYLFFSTKEEIGFAIGKSPRAVVGIKDENFSKKIIELIE
                     I"
     gene            14267..16207
                     /gene="infB"
                     /locus_tag="L3M60_001067"
     CDS             14267..16207
                     /gene="infB"
                     /locus_tag="L3M60_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBN6225756.1"
                     /translation="MSKTRVYQIAEELNISNEELINKLAELDINVTDKDSVLEGDELE
                     LALEMLGEDLSQENGNIIEIDGKLTVQVLATKLDKSPSEIIMKLMKMGTMATINQEIS
                     FEIAALAAKDYGFELTVAESDDTEALEIEALMEIEEDKEEDLKPRPPVVTVMGHVDHG
                     KTSLLDAIRKTDVISGEAGGITQHIGASEVKINGHKIVFLDTPGHEAFTSMRARGAQV
                     TDIAILVVAADDGIMPQTVEAINHAKAAGVPLIVAINKIDKPGANPDKVKQELADQGL
                     LVEDWGGEVIAVPVSAKKKEGIDTLLEMVLLVAEMEELRANPNKRAVGTVIEAELDKG
                     RGPVATVLVQGGTLTVGDPIVAGVACGKVRAMINAKGKRVKTAGPSTAVEILGLSEVP
                     QGGDQFVEVPTDKIARSVAARRQQIVRDEMLKSTQRLSLDALFSQMSEGSIKDLNIVI
                     KADVQGSVQAVKQSLEKLSNEEVQVKVIHGGVGAVTESDILLAAASNAIIIGFNVRPV
                     PGAESLGEKENVDIRTYTIIYKAIEDIQAAMTGMLDPEYVDEETGKAEIREIYKISGV
                     GTVAGCYVTNGKIFRNCKVRLVRDSIIIHEGELAALKRFKDDVKEVNSGYECGMSFVN
                     YNDIKEGDIVEAYITKEVERKL"
     gene            16250..16630
                     /gene="rbfA"
                     /locus_tag="L3M60_001068"
     CDS             16250..16630
                     /gene="rbfA"
                     /locus_tag="L3M60_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBN6225757.1"
                     /translation="MASYNRTRRISEEIRKVVSTMLINGVKDPRITSMVSVTDVEVTN
                     DLRYAYVYVSILGGDEESTLTGLKSAGGYIRREVGKNIKLRYIPEIVFKIDDSIEKGM
                     YMDSLIKRVNEKNAQQNEDENYDE"
     gene            16611..17594
                     /locus_tag="L3M60_001069"
     CDS             16611..17594
                     /locus_tag="L3M60_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HBN6225758.1"
                     /translation="MKIMMNNMDSIINYIYEGDYFIVTSHISPDGDNIGSTLSMYYTL
                     KNLGKNVYYVLDDEAPLNLGFLVEGVKIYKSNEFNMKNYSIIALDCGDKMRICVSDEI
                     KNNASKLICIDHHASNDSYGDLNYVDIDASSTCELVYNLLVRFQQIKDINIINEDIAT
                     CLYTGLVTDTGNFSYSNAHASSFEMAKNLLVLGAQKNTIIQNIYQSNSYDYYKLLGEA
                     LKALEIFNSKVSSIVLTQDMMNRNNISFNDVDGITTYTRDIKGIEVGILFKEKKQNEI
                     KVSFRSKNYIDVSEIAKLFGGGGHVRAAGCTIRDSIDNAKKMVLEAVLKSI"
     gene            17607..18512
                     /gene="truB"
                     /locus_tag="L3M60_001070"
     CDS             17607..18512
                     /gene="truB"
                     /locus_tag="L3M60_001070"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBN6225759.1"
                     /translation="MNKIISILKPTGMTSHDVVSRVRKILNIKKVGHTGTLDPDASGV
                     LPICIGKATKVCEVILNKDKSYICELTLGISTDTYDASGEILKKVDDFKFSNEEIKRA
                     FDTQRGEINQLPPIYSALKVNGKRMCDLVRSGRQSEITLKTRRVNIKNIKILSIKGNK
                     VMFYVECSKGTYVRSICHDIGEYLGCGAHMSFLNRTSSGKFDLDNSITLEELELFYEN
                     KTLDKYLYDIDYVLDSFNYVVLNSNAIKYYSNGGSIDDKRFLKNNFNKDDEFVRVYST
                     DNFLGLGKLSKHNNIISVKSDKMFI"
     gene            18642..19571
                     /locus_tag="L3M60_001071"
     CDS             18642..19571
                     /locus_tag="L3M60_001071"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBN6225760.1"
                     /translation="MEIIKSIKEIANIKESVITIGNFDGVHKGHQVLIGKTVEHAKKE
                     NIQSIVFTFANHPVNYFRPNSTKNIISNDDKIKLLEDLGIDIVVIIPFDEYMTKIPAK
                     DFVDEILVKKLKAKKIVIGHDFTFARAKEGNVNLLKSLEHEFGFEVEVIKPIKINDVR
                     VSSTYIRSLVSQGDMASVKEYLGRNYKLEGCVIHSKHLGRTIGFPTANIDLKNNMLVP
                     KRGIYASIVHIGDETYFGATNVGYNPTVNGKTLSIETNILEFDRDIYGENIIVEFLER
                     IRDERKFNSIDDLKNQLYNDTNYVYENYVCKNK"
     gene            19675..19932
                     /gene="rpsO"
                     /locus_tag="L3M60_001072"
     CDS             19675..19932
                     /gene="rpsO"
                     /locus_tag="L3M60_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002843739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBN6225761.1"
                     /translation="MINKSEIIKEYGRAEGDTGSPEVQIALLTARINELNGHLKTHKK
                     DHHSRRGLLKMVGRRRNLLAYLKEKDLEGYRALIAKLGLRK"
     gene            20172..21017
                     /locus_tag="L3M60_001073"
     CDS             20172..21017
                     /locus_tag="L3M60_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBN6225762.1"
                     /translation="MKIKLVCDSLCDIPDEISEKDYIEIVPLTVIFNDREYIEGVDIK
                     KEEFYKKVKEIKQIPKTSQATYMEFKEVFDKFIAEGYHIICMTGAANASGTFQSAMIA
                     KNDVDENEKIHIFDTRNLSLGSGQYVIKACELLEEGLGFEKIIDELENTRSSVRLLFA
                     PYTLDFLKQSGRVPVATALIGNMLNIKPIFFFDNGEAKLVNKVRGIKNIASKLVDIIL
                     EMNEGSLEGKIITIGCGDNLHDCEILKDEVNKRIKARSVLFTRGGVSICSHTGPDILA
                     ISCSN"
     gene            21142..23253
                     /gene="pnp"
                     /locus_tag="L3M60_001074"
     CDS             21142..23253
                     /gene="pnp"
                     /locus_tag="L3M60_001074"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBN6225763.1"
                     /translation="MFEHKIFKMDFAGRELSVEIGKICEMASGSCIVRYSDSMVMVNT
                     TKSAKPRDGIDFFPLSVDYEEKLYSVGKIPGGFLKREGKPSEKAILTSRLIDRPIRPL
                     FPKGFRNDVQVVATVLSVDQDCTPDIVAMIGSSIALSISDIPFNGPTGSVCIGLVDGA
                     FVVNPNAEQREKSSMHLVVSGTKEAIMMVEAGADEVPDEVMLDAILFAHQEIKKIVEF
                     IEGIVAEVGKEKMPVELYHAGEEITQLVREFATDKMKKAVQTFEKLERMENMDRVKEE
                     TLAHFEETLEDFEDFVGDIEEVLQDIIKEEVRKLIVHENVRPDNRKLEEIRPIWCETG
                     MIPRAHGSAIFTRGQTQVLNVATLGALGDVQKLDGLDEEENKRYMHHYNFPAYSVGEA
                     RPSRGPGRREIGHGALAERALLPVIPSQEEFPYAIRLVSEVLSSNGSTSQASVCGSTL
                     SLLDAGVPIKDMVAGIAMGLIKHDGKVAVLSDIQGMEDHLGDMDFKVAGTEYGITAIQ
                     MDIKIDGIDKEILQRALKQAKEGRIHILGEMRKTISQPKPELSPYAPKIVKMQINPDK
                     IKDVIGPGGKIITKIIDETGVKIDIEQTGEVFISGIEIDMIKKAQELINNIVVEPEVG
                     KTYKGKVSRIMNFGAFVEILPGKEGLLHISHIAHERVAKVEDVLNIGDEVEVKVTEID
                     EKGRVNLSRKVLLPKPEHKNK"
     gene            23376..24119
                     /locus_tag="L3M60_001075"
     CDS             23376..24119
                     /locus_tag="L3M60_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6225764.1"
                     /translation="MIMMVLNKSKLKKIIILILVVLLVLSGGVMVLKLFNNKPVFNLF
                     NGVDLPYERGTKDESGYVALTCNIDLGWETEYVESILETLKKENVKITFNVTGKWAEK
                     NKDELLKIKKQGHEIGNHGYKHLDYSTLSYEDNYEQIETSKKIIEEIIGEKTKFFQAP
                     AGSFGPETVKAAKALGYTSIKWDADTIDWKYKDQPEVIIDRMKKKDIKDSSIILMHPT
                     NATTKCIDDIIAIVREKGLKPGKLSDVFK"
     gene            24277..25524
                     /locus_tag="L3M60_001076"
     CDS             24277..25524
                     /locus_tag="L3M60_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBN6225765.1"
                     /translation="MYKTKILENGLTIIGEEIPYLKSITLGIWINAGSRIEEAQVSGT
                     SHFIEHMMFKGTKNRTSKEIASSIDNLGGQLNAFTSKECTCYYVKLIDEHIDIGIDVL
                     SDMILNSKFDKNDIDKERLIILEELKMYEDSPDDLSYDLLVENIYANDGLGMNIIGTK
                     ESLYNITRESMLEYLNKYYIPNNAVISIAGNFNFDDMVEKIKSKFGHWEKKNLSIDIS
                     EAKFNPCFISKNKDTEQVNLAMCLKGIPFENDEEVYSMAVVNNIFGGSISSRLFQKIR
                     EEKGLVYSIYSSQTLYRKCGELGIFASMSTENLQDVYNLVKKEIENIRENYLTEEEIS
                     ESKEQLKGNYILDLESTSSRMMSTGKSMLLSKKVKTTDEILECINNVDINSVKKVVDK
                     VFNVENIGTCIVGRDVEKILHLN"
     gene            25559..25804
                     /locus_tag="L3M60_001077"
     CDS             25559..25804
                     /locus_tag="L3M60_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBN6225766.1"
                     /translation="MNLSEIAGKEIVNLVTGERLGVIGECDLILDETTGGILALLIPK
                     EKGFFARRKDKSFLEVPWRNVKKIGNDMIIIEYEGYI"
     gene            25814..27037
                     /gene="dapG"
                     /locus_tag="L3M60_001078"
     CDS             25814..27037
                     /gene="dapG"
                     /locus_tag="L3M60_001078"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBN6225767.1"
                     /translation="MSILVQKFGGTSVESYEKMNEVCKIVKAYKKNDEELQLVLVVSA
                     MGRKGAPYATDTLINLCSAVNDEPSKRELDLIMSCGEIISGTILANLLNAQGIDSVFF
                     TGQQAGIITSDEYSNAKIKYINPKKIKRALDDGKVVVIAGFQGVTDDGEITTLGRGGS
                     DTSAVAIGKALECETVEIYTDVDGIMTADPRVEPNAKVLSFIDYEEVFQMADKGAKVI
                     HPRAVQLAKSGNITLAIKNTMNPTFEGTKIGSLCRHLEDNIEYEQERDFKVAVANKDS
                     VAQVKIKSAEEVFTEVLNEIEKKFITIDMINFFISEKAFVVEDADIKSLKEILDKFEL
                     DYEVNRDCAKVTLICSRIADEMSGIMSKVVRGLSKAGVSLLQTSDSNMTISCLVSEED
                     MHTAVHAIHQQFYLK"
     gene            27151..27885
                     /locus_tag="L3M60_001079"
     CDS             27151..27885
                     /locus_tag="L3M60_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HBN6225768.1"
                     /translation="MKDYREDNERFDKDNCSEESSSEERECIKQFGTNEMPPQPPKDI
                     QCITIIGEIEGHFIGNPQKKATKYEHIIPMLYSIEESNDVKGVLVVLNTVGGDIEAGL
                     AIAELLNSTSKKVVTLVLGGSHSIGVPLATAGDYSFIAPTATMIIHPVRTTGLVIGIN
                     ETFEYFKKMQDRIIQFIIRTSNIKKDVLEKLMHEKDELVSDVGSVLIGKEAVDYGLID
                     EVGGLKEALKKLRELIKESEEEKNND"
     gene            28008..30416
                     /locus_tag="L3M60_001080"
     CDS             28008..30416
                     /locus_tag="L3M60_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBN6225769.1"
                     /translation="MATKKKKKKKVNTLSFNAEYHNLITIFIGVFLLYSLNSNSMGWI
                     PVLMQNLFKGLFGGLSIAIPFIVIITGLLGFFDGNEYIYRLRKTKLYYIIILFIFVFY
                     GLLNAGTLPVDSPLKGNMFDDVMKLGVSGQGSGLIATTIAYYMSKIFGIVGGWLISIF
                     ALILSVMFIFNISIKDMISNAKSKSKASKDSNLTFKDKIANMKKSAIDMMTDEVDDTT
                     INKKPGFFKGLMSKGRNTDEEDEYFEEENSDGVDDKTIKIVGFNKAEDEYLEILEGTQ
                     SMPELDVLKELQKATNENPVIDTKPEKKVDIAKPNLNIEKTQPMSIVAEPVNEDYSNY
                     KKPSIELLNKVNKKSDENGKKKVLKNASLLEKTLSDFGVEAKINQVTVGPTITRYEIQ
                     PSPGVKVSKIVNLTDDIALSLAAKSIRIEAPIPGKSAIGIEVPNEEAQMVGVREVLES
                     EEFNNFNSPLAMGLGKDVAGKIIIGDIGKMPHLLIAGSTGSGKSVCVNTLISSILYKA
                     NPDEVKLLLIDPKVVELANYNGIPHLLIPVVTDPKKAANALNWAVTEMNRRYKLFADA
                     QVKDVTSYNEKAEEKLPKIVIIIDELADLMMASANDVEDYICRLAQMARAAGMHLIVA
                     TQRPSVDVITGVIKANIPSRIAFAVSSQTDSRTILDMGGAEKLLGKGDMLFYPLGAAK
                     PVRLQGAFISESESEKVIDFVKSQVKDGIKYEEDIIETISKVNTSKGSDEDEFLSEAI
                     EFVVESGQASASMLQRRFKIGFNRAARLIDSMEERGIIGASEGSKPRKVLISKQDLQN
                     LEGE"
     gene            30419..31477
                     /gene="mnmH"
                     /locus_tag="L3M60_001081"
     CDS             30419..31477
                     /gene="mnmH"
                     /locus_tag="L3M60_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBN6225770.1"
                     /translation="MSVIKIEKALKLDKVIFVDVRTEGEYEEDHILNAINMPLFKNNE
                     HNEVGTIYKMQGKHEAIQKGFDYVSYKLKDIYLQAAELALNYDNIVIYCARGGMRSGS
                     IVNLLSSLGVNVYQLEGGYKAYRNFVLEYLRHVMDTKNFVSVHGLTGTGKTDLLHLLD
                     EKGIDVLDLEGIAKNSGSTFGFITFDKKPPSQKNFETKLFESIFFSKENYIFVESESK
                     RVGSVTIPNEIFEAMTREGHHILLECDIENRVDRLCRDYIYDKDEGNLLVLKECINKF
                     RKRLGHKVVDDYIDLLESGEYKELVKRYLLEYYDPLYMHSVEKYKYNKVINFNDTEEA
                     LEDVIDLYESILEGENEC"
     gene            31471..32805
                     /gene="rimO"
                     /locus_tag="L3M60_001082"
     CDS             31471..32805
                     /gene="rimO"
                     /locus_tag="L3M60_001082"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBN6225771.1"
                     /translation="MLKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIV
                     NTCGFIESAKQESIDTIINFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVG
                     TGSYQNIDKILRELSEMHQIVSLNDIEFVFNEDLPRYLSTPSYMAYLKIGEGCSNNCT
                     YCIIPKLRGKYRSRKFEDIIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELL
                     EELAKIDGFKWIRVMYSYPESITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRK
                     TTKEDILNKINLIRSNIPDAILRTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAY
                     SREEDTPADRLPNHIDEDVKIQRRDTLMMIQQKISEELNDKKIGKTYEVLIEEQIEDN
                     VYTGRTQGDAEEIDSIVYVKSVDNLEIGEFVSVKINDAMEYDLMGDVLYELA"
     gene            32792..33334
                     /gene="pgsA"
                     /locus_tag="L3M60_001083"
     CDS             32792..33334
                     /gene="pgsA"
                     /locus_tag="L3M60_001083"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBN6225772.1"
                     /translation="MNLPNKLTLFRIFLIPVFVLIMLLNIPNKFLIACIIFIIASITD
                     ALDGKIARKYNLVTDFGKFMDPLADKLLVISALTCMIEDHLVSSWMVIIIVARELTVS
                     ILRAIAAADGKVIAAGNSGKLKTITQMVSIVLLLLGAQFANGLILNIGEILILIATLL
                     TLYSGWEYLYKNKELFMSSK"
BASE COUNT        13530 a         3490 c         6286 g        10080 t
ORIGIN      
        1 tgatgcagaa aaaatgtatt taaatgtaaa tccagatttt acaaaagatt atgttttggg
       61 aaatataagg atacatataa aatcaagaat aaaagattta tttaatatta taataatgat
      121 taataaaatt atgaataaga ataaaggaaa taaggaaggt gatagtaatg agagcaacag
      181 gttcaataca gagtcttatg gaaacaactc ttgaaacaat taaaggttct atagatgcaa
      241 atactattat aggagaccct ataaaaacag atactacagt ggttgtacct atatcaaagg
      301 ttacaattgg ttttggtata ggaggaggag aatattcaaa aggatatgat gataaagata
      361 gggaaattga attaaaaaat gaaaaatcag atacgaattt tgctggaggt agtgcagggg
      421 ctatatctgt acaaccagta gcgtttgtag tagttgaaag tggagaaact agaataatga
      481 gtttagatag caatatcaat ctagtggata atatattatc tataactcct agagtattgg
      541 agaaaattca aaatatatca caaagtaata agaatcaaga taaaaacaat atgtaagaat
      601 aaaatatgta tatggtttta aataaagtag tggggttagt ataaatccca ctattttgct
      661 gtgtacttta aacatttata tctaataaga ttttagttta aaacaattta aatagataat
      721 aaagttgata tatagtataa ttataaaaag aaaagaagta ttatattata gatatataaa
      781 cacatttgag atataagagg gagttataaa tcattagttt tgaggtgaat gttatgagcg
      841 ataaagaatt atctattttg ttgttagaaa gtatgaataa ttttcataat ttaataaaga
      901 ttattaataa cgaaagatat aaaaaagata aggttcttac agaaagacag ttctttgcct
      961 tagtaaaaat aagaaaacat gataaaatag agcttaaaaa cctaagtaga gatttacatg
     1021 tatctacttc tagtttgtgt atactactta ataaattggt tgagcaagag tatgtatata
     1081 gagaagaaga tagtagagat agacgaaata ctttttatgg aataactaaa aatggagaaa
     1141 agatattgga taatgaaata ctaaagtttg tgtctataat aagtgataaa atggattgct
     1201 tagatataga taataaagat aaattattta cttctttgga agaatctaaa aatataatag
     1261 aacaactatt ttaaaaagga gaatttatta tgaatataaa gtgttctaaa gaaatattaa
     1321 aaagtttaga aaatgctata acagtagtag ctctatttga gggagaagat gtttctacga
     1381 catttgataa aataaatatg ataagcagaa attttaattc taaagaaaat agtatagata
     1441 ctgtaacatt ggttgaaaat aacaatttgg taaatgtttg tcttgttggt tttggtaaga
     1501 gagagttatt gaatagagaa aaaattagaa tcataggtgg gaatttgtgt aaaaaattaa
     1561 aaaatatatt gaaaaatgaa gtatataaga gtagtgatgt taatgtactg aatcttaatt
     1621 taaatagtga tgaaataggt tcttttgcag aagggatatt acttggaaat tataagtttg
     1681 ataggtataa gacaaaaagt aagaattttg aaagaaaaga agttgacact ataactatat
     1741 ttactgaaaa agacgttgag tgtcaaataa aaaaggctaa aatattagct aattcaacta
     1801 taatagctag aaatctagtt aatgaaccat caaacgtaat atacccaaaa acattagcaa
     1861 tagaaacagt caaacttggt gcagagtttg gatttaatgt tgatgtttat gaagaagaaa
     1921 aaataaaggc attgggaatg gatgcttttt tcgctgtttc aagaggttca attaataaac
     1981 caagattcat agtaatgaga tactttggag acaaagaaag tacggatata ttaggtctag
     2041 taggtaaagg attgacttat gatactggtg gatattcttt aaaatcaaat gcttctatgc
     2101 tagatatgaa aaccgatatg gctggtgccg cgagtgttat aggtgctatg tgtgctatat
     2161 ctcaaagtaa attaaagaaa aatgttatag cagtagtggc tgcatgtgag aatgctttat
     2221 caggaggttc atacaaacct ggagatataa ttaattctat ggcaaataaa actattgaag
     2281 ttttaaatac agatgctgaa ggtagactta cattagctga tgcaatatac tacattataa
     2341 acaatgaaaa agtgacaaag gtggttgatg tagccacatt aactggtgct gccttaacat
     2401 tgttaggcaa tgttgcaact cctatagtaa caaataatga tgatttttat tgtgaattag
     2461 aaaaagccgc cactttatca ggagaaagag tatggaagat gccaatttat gatgaattta
     2521 aagatatgat aaaaggagaa gaagcagacc ttaaaaacac tggtggtaaa aatgctggat
     2581 gtataactgc aggagctttt attggtgaat ttgttgggaa tactccttgg atacatatgg
     2641 atatagcagg gacaagtaca tcatcaaaat caattggata caaagtaaag ggggctacag
     2701 gagaacctgt gagaacattg tactatttgg cagaaatcta aaacaacaat tatagaatat
     2761 aatttataaa gccacgtgga tatttttgta atttaaggtt atctatgtgg ctttattttt
     2821 tatttaagta aattatttac tctttttctt tttttcaagt ataaaagtat ctctattaaa
     2881 taaaagagca ggtatgtaca taatactagt aacaaaaata aatgtaaaaa tagcagttgt
     2941 actatggctt aaaaaaccat aatagtattg tgaattggta agtgtgttta gtaaaaatat
     3001 gaatgaaaaa taaataaaac atatagctaa gttatctcta agtgcatatc tgttaacttt
     3061 aaattttcta acatcaacca tatatatatc tgcataaatt attaagcact tagagaagat
     3121 aaatataaaa tatattaagc taggaaattc ataacccaag tttgtagaaa aaataagttc
     3181 tccaactgaa caaactaaac tccaagaaaa aaatatatta aaaagatgat actgcttaaa
     3241 aatcagtata gctatacaca gatatatgca aatataattg attccaattg gtaaagaatt
     3301 tagggtacta taattacctg aagaaataag taataattgc tctagagcca taattattac
     3361 aagcaatgta caaattatct tctcgactgt atagctataa ggtaaaatat ttttggttaa
     3421 ttttggacaa tagtataaaa ataatccaaa tataaagaga cttacaaatt gttcactaga
     3481 aaaaagaaaa attttaaaca tataacattc tctcctataa taaatttaat atgtatctat
     3541 ataaatatat tcattatatc tcaatttaat atgtaaaaaa tataataata gttagatttt
     3601 ttatatcaat tttaatatgt aaaatatcct attgttttgt agatatatcc taattatgtc
     3661 aaaaaataaa aaagttttta tttattagag tttttggaaa taataataac agatattata
     3721 attaattaac agtttttgta agtatttgac aatagagagg gtgtaacaaa tgggaataat
     3781 agagtcagca tcaaaattgg ctgagatggt tcatttattg gctgttgaaa aagggataac
     3841 tgatatagaa gcatgggatg aagctgtgaa agaatacagc aaaatttacg aagaaagaag
     3901 aaatgaatag aatgataaaa agtgacaact tgatgataaa aaataactct atttaaataa
     3961 attcaatttt tatagaaaca ttaaaaataa tataaataaa aagatggtat ttcaaatgta
     4021 agtaaaaaat cattttggga taccatcctt attatttatt ttaataatat ataagtaata
     4081 aattttactt atcaatattt tttatgtaac ctcttttttc tagctttttt atatgctctt
     4141 tgcgtatttc atcaagatta ataccatact gttcttctag cttaaacatc atagtaacac
     4201 atgtctgagc tacatccatt agttccttag cagttttctt tataatatca acttcatcta
     4261 agtcattatt ttctccactt agatttctaa atttacctat acattcagct aattcacctt
     4321 gttcttcacc cattttgata aatgtagatt ctagggttgg gtctaaatta ttaagctctg
     4381 gtaaagatat agtttttagt ttcataaaag ctcctttcaa gttattcata tttatactaa
     4441 aacatcataa caaaaataat actaaaaaaa aactttaaat tcaacataaa aacttacata
     4501 tataataaaa atcgtgaaat aaatctatat ttgtcttaat acagcttcat ataaaacttc
     4561 tccaataggt ttatttatta caagagaagc tgacttatca tagactgtat ttgctttatt
     4621 aataagagca atataattac ctttgtaata atctataaaa cttgctgctg gatatacagc
     4681 caatgatgta cctcctataa ttagtaaatc agcattactt atagcagaaa ttgttttagt
     4741 aatgatatca ctatcaagag cttcctcata aagtactaca tctggtttta ctatagaacc
     4801 acagttatca cagtaaggaa tattaccacc aagatttaac atagattcta agtcaaaaaa
     4861 cttaccacat ttagtgcaat aatttctatg tacagagcca tgaagctcta ggacattttt
     4921 acttccagcc atttgatgaa gtccatcaat attttgagtt ataacagctt taagtttacc
     4981 catttcttcg agttttgcta aagctatgtg agcattgttg ggttttgcat tagggtatat
     5041 taatttgtcc ttataaaatt caaaaaattc ttctgggtat ctaacaaaaa aagtatgtga
     5101 aactaattgt tcagcagtaa attgtttgtt gagtttttgg ctaaataatc cagtagagct
     5161 tctaaagtct ggaatattag attcagttga aacacctgct ccaccaaaaa atacaatatt
     5221 attgtgatta gctattaagt cttttaagct atttgcatcc atagttacac ctccaaatta
     5281 ttatatataa attgtactac atattaaggt atttatgttt tattatttca aataaataca
     5341 tagtttaata agttaaataa agggaatata aataaaatgt atacataata taaataggca
     5401 atatttacta aaataacaag ttgtcgtata attatattaa aattgtttta gtgtataaga
     5461 agggcgggga agtacatgaa gaaaaaagca gctttagcaa cattagctat gttaccctta
     5521 agtgtggtaa atgcacatgc tgatggagat ataggcatag tgactataaa ttatttaaac
     5581 gtaagaaatg aaccaactgc tgaaagtagt atagcctttg ttgctaaaaa agatgataaa
     5641 gttttgatta aagactcttc taatggatgg tataaaataa aagctgaatc tggacaagaa
     5701 ggttgggctt catcaaaata tatagcaaaa ttaaatggtg actctttaag aacatctaca
     5761 aataaagaaa aacaagttac ttcaaatagt ttaaatatga gaaatggagc aggtacaagt
     5821 tatagagtaa taactgtact aaaaaaaggt caaaaagtag aagtaatatc agagagtaat
     5881 gggtggtcta aaatcaaata tgatggaaga ttagggtatg tatctagttc ttatttagga
     5941 gatgtttcaa attcaactaa taaatcaaaa acaaaacaag tgaatacaac ttcactaaat
     6001 gtgagaagtg gaccaaatac aagttatggc ttgttaggta agttgtcaaa aggaagtaaa
     6061 gtagaagtaa tatcagaaag taatggatgg tccaaaataa agtataatgg aaaagatgca
     6121 tatgtatcta gtatgtactt atcagatgta agtcaaagta attcagacga ttctagtcaa
     6181 agcaatgata aaaaaaatac tgataagttt gtaaatacag cttctttaaa cgtaagaagt
     6241 ggaccaggtt ctacatatag caagttggga aaggtttata aaggaagtaa ggtaactgtg
     6301 ctatcagaaa gtagtggatg ggctaagatt aattttaata ataaagaagc ctttgtagta
     6361 ggtaattatt tatctacttc agcagatgca tcaaataata actcaaatag taactctgat
     6421 agcagttcta gtaataatgg taataattca tcatcaggtc aagtaaatgg tatgtcaggt
     6481 ataagtggag ctaaaattga ttataaatct ttgaattata ctttggcatc tcatataagt
     6541 aaacaagttg agaaagcagc atcaggagga aatgtgatag caccaagtaa taggaaaagc
     6601 accccaagtc ctgaatttag tactttttca gcccaaagaa caagctcatt tgtaaatgca
     6661 agttcaagtg atatagaata ttatttaaat cctaaaaatt ttataaatac tactaaaggt
     6721 atgatgcagt ttttaaagat taacagttat agaggtggta tttcagaatc aagtcttaac
     6781 tcgtatctta atggtttatc ttctagtgta tttaaaaacc agggagcaac atttataaat
     6841 gctgcaaaga agtataatat agatgttgta tatctagtat ctcatgctat gtgggaaact
     6901 gcttatggta agtctacact tgctcaaggt cagacattaa catcttataa gggtcaacct
     6961 cttagtaaac cagttaaagt atacaatttt tttgggatag gagcgataga taaaagtgct
     7021 aatgtttcag gtgcagaggc agcgtattcc aatggttgga ctagtgtaga agctacaata
     7081 gatggatctg caaagtggat atcacaaaat tacataaaca gttctaagta caatcagaat
     7141 actatctaca agatgaagtg gaattatgat tatacatggc atcaatatgc aactgatgta
     7201 aactgggcta atgggatttc tggaattatg gagaatttaa ttggtcttta tggaggagga
     7261 agtagtttgg tttttgaagt tcctcaatat aagtaatata tgaataaaaa atatagacta
     7321 tgggtagttt tcattttatt taaattatct atagtctttt tgtatataat ttacatgctg
     7381 taaatttaat gttaacaaaa tttttatgct ataatattaa ttctaagcaa atataagtga
     7441 gtaatagata gaaatagata aaaagtggta ataatatata tagataattt tgagaaagga
     7501 ggatatagat ggaaagtatt aaggaatatt tggataagtt agaaattaat aatagtggtc
     7561 ttggaaagca acttaaagaa gtttacataa atagagtagt ttattttaag gaagataaga
     7621 tagtttattt ttatctgact tctaaggata ttgtttctca tgagctttta gataaattta
     7681 aagaagaatt gatgtataaa ctcgattatt ttaaagatat gaggatgaag ataagattta
     7741 ctggtcttga aagaaaatcc aataaagatg ttataaaaaa atattggaat aatatattgt
     7801 atatattaaa gtatctttgt ccatctattg caggatggta taaacaggta gaatttcttt
     7861 gtttagagga agaattaaaa ataaaacttc caaaaggcat attttatgag cgattaatga
     7921 aaaaaaatgt aacctatgta cttaaaaccg tgcttagtga agaactggga ttagatttaa
     7981 atataacaat agaaaaagcg gttgatgaaa agataaataa agagagactc ataagaatca
     8041 atgatagaga aatggaagaa aaaattagag ctttagaaat tggaaaagtt aatagctgtg
     8101 aagaaagtga agaggaaggt tacgtcatta agtctgaagt agatgaagaa ttaatctatg
     8161 gagataatgc aaatgctatg attgaaaata tagttgaatt aaatgcatct tctggaactg
     8221 ttgcagttgt tggagatata tttgatgttg atactaaaga gcttaaaaat ggaaaaatac
     8281 ttatgatagc atcaattaca gattatacta gttcaataag ttgtaaactt tttttaacag
     8341 atacaaataa agatggagta cttgaaaatg ttaaaaaagg agcatactta aaaatcaagg
     8401 gtgacatagt ttatgataca tatcaaagag aaattagtat gatgattagt ggtataagaa
     8461 aagaaactag aaaagagaga gaagataaat cagaggaaaa aagggtagaa ctacatgcac
     8521 atactcaaat gtcttctatg gatgctattt gttctgttaa aaagcttgta gaacgtgcgg
     8581 ctaaatgggg acatcctgct attgctataa cagaccatgg agttgtacaa ggatttccag
     8641 atgccatgaa tgcaggtaag tcaaataata taaaaatact ttatggagta gaagggtact
     8701 tggttgaaga ggattctctt attatagagg atgcaaatga taaagaactt tctcagacat
     8761 ttgttgtatt tgacatagag acaactggtt tttctaatac aaatgataaa ataactgaaa
     8821 ttggtgctgt aaaaatagaa aactttaaaa ttgtagatag atttagtgaa ttaataaacc
     8881 ctgaaaaaga tatttcttat aagatacagg aattgacggg aataactaat gagctaatta
     8941 aagataagcc gaccattgaa gaagttcttc ctaagtttat ggaatttgtt ggagatagtg
     9001 tattagtagc acataatgcg gaatttgata ctggatttat atcgcaaaaa tgtagggaac
     9061 aaggtttgtt atataataac aaaaaagtag atacacttat gctagcaaga gtaatgttac
     9121 caaacttaaa aagacataga ctaaatgtcg ttgcaaaaga acttggtatt cctcttttaa
     9181 atcaccatag agcagtggat gatgctgagg ctacagcact tatatttaat aaatttttgc
     9241 agatgcttac ggaaaaaggt gcaaaaacat taagtgatgt aaacaatata cttggaaaga
     9301 ttgattatac aaaacttagc acaaatcata taaccttgat agcaaaaaat tctgtaggta
     9361 taaaaaattt atataaaata atttcagatg cacatgtaaa tcattttttt agagcaccaa
     9421 gaatccttag aagtgtgtta aatgaatata aagaaggatt aataattggt tcggcctgtg
     9481 aagctggagt ggtttttcaa gctgtaaaga aaaatgtatc tgatgaggaa atgaaaaaaa
     9541 taatagattt atacgattat attgaagtaa tgcctataga taataatagg tttatgattg
     9601 ataaaggaga agtaaaggac gaagaagagt taagggaatt gaataggaaa ttaattgata
     9661 cagctaaaaa atttaataaa attccagtag ctactgggga tgttcatttt ttagataaac
     9721 atgaagctat cttaagaaaa gttcttaaat attctcaagg ttttaaagta gatgaagaag
     9781 aaacttattt acattttaga acaacagatg aaatgttgga agagtttagt tatcttggag
     9841 aagaattggc atatgaagtt gtagttgaaa atagtaatct gattgccgat atggttgaag
     9901 atataaaacc tataccagat gatacttatc ctccaattat agagggttca gatgtggagt
     9961 taagagaaat gtgttacaaa aaagcaaaac gaatctatgg agacccaata ccagaaatag
    10021 ttcaaaaaag actggataga gaattaaatt caataataag caatgggtat gcagttatgt
    10081 atattatagc tcaaaagcta gttgcaaaat ctctttcaga tgggtattta gtaggttcaa
    10141 gaggttcagt tggttcatct tttgcagcta ctatgagtga tataacagaa gttaatcctc
    10201 ttccagcaca ttatatttgt gaaaatgaag actgtaaata ttcattcttt tatgaaatag
    10261 gtgaatgggg ttcaggtgtt gatttaccag acaaagactg tccaaagtgt ggaagaaaac
    10321 ttgccaaaaa cggtcatgat attccatttg aggttttctt aggttttgaa ggtgataagg
    10381 aaccagatat agacttaaat ttctcaggaa attatcagcc aacgattcac aaatacacag
    10441 aagaactatt tggagagggc tatgtatata gggctggaac aataggtact gtagcagaaa
    10501 aaacagcttt tggatatgca agaaaatatg tggaggaaaa taatataagt gtaccaaatg
    10561 cagaagtact taggctttca aatggatgta ctggagtaaa aagaacctca ggacagcatc
    10621 caggtggagt tatggttata ccagattata aagatgtgta tgattttaca cctatacagt
    10681 atccagcaaa cgatacttct tgtggtgtta taactactca ctttgattat cattcaataa
    10741 gtggtagaat acttaaactt gatatacttg gacacgatgg tcctaccatt ataaggatgc
    10801 ttgaggatat gactggaata aatataacag aaattccgtt ggatgataaa gagactatgt
    10861 ctctatttac atctacagaa gcattgggtg ttacaccaga agaaataaac tgtcctatag
    10921 gttgccttgc tgtaccagag tttggaacta agtttgtacg acaaatgtta cttgacacca
    10981 aacctactac atttgcagaa cttgtacgta tatctggtct ttctcatggg acagatgttt
    11041 gggtaaataa tgcacaagac ttagttagag aagatatagt tggcttaaaa gatgttatat
    11101 ctacacgaga tgacataatg aactatttaa tatttaaagg attacctccc aaaatgagtt
    11161 ttacaataat ggagagcgta agaaaaggta aaggtctaaa accagaacac attgcggaga
    11221 tgaaaaaaaa taatgttcca gaatggtata taggctcatg taaaaagatt aagtatatgt
    11281 tcccaaaagc acatgctgtt gcttatgtta tgacatcatt tagaattgca tattgtaaag
    11341 ttcactatcc agaggcattt tatgcgactt attttactac aaaagttgag gattttgatg
    11401 cagatttaat tgttaaagga ttagatgcta taaaatcaaa aataaatgaa attgagtcgc
    11461 tgggaaatga tgcaaccaca aaagaaaaag gtatgttgac tgttttagaa gttgctcttg
    11521 aaatgtatgc tagaggaata aaattattac cagttgatat atataaatca gatgcgactg
    11581 agtttataat tgtaggagaa aaaacattac ttccaccaat ggcagcaata caaggtctgg
    11641 gaggaaatgc ggctataaac atccaaactg aaagaaaaaa tggtgaattt atatcaaaag
    11701 aagatttaag aaagagaaca aaaatatcaa aaacggtaat agaaactctt actaatcatg
    11761 gttcacttga gaatatgagt gagagaaatc aattgtcatt gttttaagtt agcccaaaaa
    11821 gttgcaaata aaagaatttt atgatatcat agtatagaaa atagtatact ttgcagaaac
    11881 taagagtgag accgctgttc tcactctttt attttaaata tattattaaa aagctggttt
    11941 aattttatta aatatatttt gtttaagatt atataaactg gtcatttaca attgaatagg
    12001 aggaatttta atgaaaaaaa atttagaagc tacaatcgag gagattgtaa ctaaaataac
    12061 agatgagcat ggatttgaaa tggttgatgt tgaatatgta aaagaagcag gagaatacta
    12121 cttaagagta tacatagata aagaagaagg aatatcttta aatgaatgtg aattagttag
    12181 tagagaatta agtccaatac tagatgaaaa agaccctata aaagagaatt actttttaga
    12241 ggtatcttct ccagggcttg atagagcatt aaaaaaagac agagattttg ttagatatca
    12301 aggtagagat gttgatttaa aattatataa accattaaat ggatgtaaac aatttgaagg
    12361 tgaacttgta ggattaacag aagataataa tataaaaatc attgtaaatg gtaaggaaat
    12421 tgaatttaat agaaaagatg ttgctattgt cagattagca ataaagtttt agtggtatat
    12481 taaataggga ggatataaaa aatgaatcat gaatttatgg aagcattaga tgaattagta
    12541 gttgacagag gtatagataa agaaatactt atagatacta tagaacaagc tcttttaact
    12601 gcatacaaga aaaactttgg ttcagcacaa aatgttagag ttgaatttga tagagacaag
    12661 ggagatataa aagtattttc tcaaagagta gttgttgatg aatcagattt atatgatact
    12721 ttcttagaaa tagagttagc agatgctaga gagataaatc ctaattatga gttaggtgat
    12781 attatagaac atgaagttac acctaaaaac tttggaagaa ttgcagctca aacagcaaag
    12841 caagtagttg ttcaaagaat aagagaagct gagagagaaa tagtgtataa tgagtttata
    12901 gaaaaagaaa atgaaatagt tacaggagag gttgctagag caaataaaaa tgttgtttat
    12961 gtaaaccttg ggagaataga aggtataatg actcaaagtg agcaaatacc atttgaaaat
    13021 tataaagcag gtcaaaaaat aaaattctat atacttgaag ttaaaaaaac taacaaagga
    13081 cctcaaatag ttgtatcaag aagtcatcca ggtttagtga agagattgtt tgaagaagaa
    13141 gttcctgaaa tatatgaagg aatagttcaa ataaaatctg tagcaaggga agctgggtca
    13201 agaactaaaa tggcagtaag atctatagat gaaaaaatag acccaattgg agcttgtgtt
    13261 ggaccaaaag gacttagagt aaaaaatata gtggatgaac ttggcgatga gaaaatagat
    13321 ataataaaat atagtgaaga cccagcagag tttatatcag cagcacttag cccatcaaaa
    13381 gttgttagag tagatataga tgaagatgaa aagtctgcct tagtaatcgt tccagattat
    13441 cagttatcgc ttgcaatagg taaagaaggt caaaatgcaa gactagcagc aaaattgact
    13501 aactggaaaa tagatattaa gagcgaatct caagcagaac aagaaatgtt aaatcttgaa
    13561 aattccaaaa aaatagaagc tgaggatgtt gaagctgaat ttatcgaaca agaagagtta
    13621 gatacttcag atgataattt tagtgatgaa gagtaaggag ggatgatttt gaaaaaagta
    13681 aaaaaagtgc ctcaaagaaa atgcatagca tgtcaagata gagactctaa aaaagagctt
    13741 ataagaatag ttaaaaataa agaaggaaaa atatttttag acccaacagg aagagccaat
    13801 ggaagaggag catatgtatg taaaagctct gaatgtctaa aaaaagctat aaagagtaaa
    13861 gcattaaaca aagcttttaa gatagatgtt cctgatgaag tctatgataa tctacttatg
    13921 gagttagaag aatatgaaaa ataataaaga aaaagtactt tcgttcttag ggctcgcaac
    13981 tcgtgcaggg aaaatagtgt ctggtgatga ttcaactttg ctcgacctta aaaaaggaaa
    14041 agtaaatttg attttaatag cagaagatgc atcaaataat actaaaaaac tttttaaaga
    14101 taaatcaact tttagaaata ttccatatct atttttttca acaaaagagg aaataggatt
    14161 tgcaatagga aaatctccaa gagcagttgt aggaataaaa gatgaaaatt tttctaaaaa
    14221 gataattgaa ctaatagaaa tttaaaataa aatagggggt gatcttgtgt caaaaacaag
    14281 agtataccaa atagcagaag aattaaatat atcaaatgaa gagcttataa ataagttagc
    14341 tgaattagat ataaatgtaa ctgacaaaga ttcagtatta gaaggtgatg agttagaatt
    14401 agcacttgaa atgttaggtg aagatttatc tcaagaaaat ggaaatatta tagaaataga
    14461 tggaaagttg acagtacaag tattagcaac aaaattagat aaatctccat cagaaataat
    14521 tatgaagctt atgaaaatgg gaactatggc aacaataaat caagagataa gttttgagat
    14581 tgctgctttg gctgccaaag actatggatt tgaattaaca gtagctgaaa gtgatgatac
    14641 agaagcttta gaaatagaag ctttaatgga aattgaagaa gataaagaag aggatttaaa
    14701 acctagacca ccagttgtta cagttatggg acatgttgac catggtaaga catctttact
    14761 agatgcaata agaaaaactg atgtaatctc aggtgaagct ggtggaataa ctcagcatat
    14821 aggtgcttca gaggttaaaa tcaatggaca caaaatagta tttttagata cgccaggaca
    14881 tgaggctttc acatcaatga gagcaagagg agcacaagtt acagatatag caatattagt
    14941 agttgcagcg gatgatggta taatgcctca aacagttgaa gctataaacc atgctaaggc
    15001 agcaggtgta cctttaatag ttgctatcaa taaaatagat aaaccaggtg caaacccaga
    15061 taaagttaaa caagaattag cagaccaagg attattagtt gaagactggg gtggagaagt
    15121 aatagcagtt ccagtatcag ctaagaaaaa agaaggaata gatacacttt tagaaatggt
    15181 attattggtt gctgaaatgg aagagttaag agctaatcca aataaaagag cagtaggaac
    15241 tgttatagaa gctgagcttg ataaaggaag aggaccagtt gcaacagtac ttgttcaagg
    15301 tggtacttta acagtaggtg acccaatcgt tgcaggtgtt gcatgtggta aagttagagc
    15361 aatgataaat gcaaaaggta agagagtaaa aacagcagga ccatctacag cagttgagat
    15421 attaggtcta tcagaagttc cacaaggagg agaccaattt gtcgaagttc caactgataa
    15481 gattgctaga agtgtagcag caagaagaca acaaatagtt agagatgaaa tgttaaaatc
    15541 aactcaaaga ttatcactag atgctttatt tagtcaaatg agtgaaggtt ctataaaaga
    15601 ccttaatata gttataaaag cagatgtaca aggttcagta caggcagtta aacaatcttt
    15661 agaaaaatta tcaaatgaag aagttcaagt taaagtaata catggtggtg ttggagctgt
    15721 aacagaatca gatatattac ttgcagcagc ttctaacgca ataataatag gattcaatgt
    15781 aagaccagtt ccaggtgcag aatctctagg agaaaaagaa aatgttgata taagaacata
    15841 tactataata tacaaggcaa tagaagatat acaagctgct atgactggta tgttagaccc
    15901 tgaatatgtt gatgaagaaa caggaaaagc tgaaataaga gaaatatata aaatatctgg
    15961 tgttggaaca gtagctggat gttatgtgac taatggtaag atatttagaa attgtaaagt
    16021 aagattagtc agagatagca taataataca tgaaggtgag cttgcagcac ttaagagatt
    16081 taaagatgat gtaaaagaag ttaattctgg atacgaatgt ggtatgagtt ttgtaaatta
    16141 taacgacata aaagaaggcg atatagtaga agcttatata actaaagaag tagaaagaaa
    16201 attataaatt cgtatcaaag aaaatcagtt agtaaagaag gtcgatggta tggcatcata
    16261 taatagaaca agaaggatat cagaagaaat aagaaaagta gtaagtacta tgcttataaa
    16321 tggagtaaaa gaccctagaa taacatctat ggtgagtgta actgatgtag aagttacaaa
    16381 tgacctaaga tatgcttatg tatatgtaag tattctaggt ggagatgagg aatccacact
    16441 aacaggtctt aaaagtgctg gaggatatat aagaagagaa gtaggaaaaa atataaaact
    16501 tagatatatt cctgagatag tatttaaaat tgatgattca atagaaaaag gaatgtatat
    16561 ggatagcctt ataaaaagag ttaatgaaaa aaatgctcaa caaaatgagg atgaaaatta
    16621 tgatgaataa tatggatagt attataaact atatttatga gggtgactat tttatagtta
    16681 cctctcatat cagtccagat ggagataata ttggctctac actttcaatg tattatacat
    16741 tgaagaattt aggaaaaaat gtatattatg tacttgatga tgaagcacca ttaaatctag
    16801 gatttttggt tgaaggtgta aaaatctata aatctaatga atttaatatg aagaactatt
    16861 ctataattgc acttgattgt ggagataaga tgagaatatg tgtgagtgat gaaatcaaaa
    16921 ataatgcatc aaaattaatt tgtattgacc atcatgctag taatgattca tatggagatt
    16981 taaattatgt agatattgat gcatcttcta catgtgaact agtttataat ttattggtta
    17041 gattccagca aataaaagat attaatatta tcaatgaaga tatagcaaca tgtttatata
    17101 caggtcttgt aactgatact ggaaactttt catattcaaa tgcacatgca agtagttttg
    17161 aaatggcaaa aaatttactt gttttaggtg cacaaaaaaa tactataata caaaatatat
    17221 atcaaagtaa ttcatatgat tattataaat tattaggaga agctctaaaa gctttggaga
    17281 tatttaattc aaaggtatct agcatagttt taactcaaga catgatgaat agaaataata
    17341 ttagctttaa tgatgttgat ggaataacaa catatacaag agatataaaa ggtattgaag
    17401 ttggaatttt atttaaagag aaaaaacaaa atgaaattaa agtaagtttt aggtcaaaaa
    17461 attatattga tgtaagtgaa atagccaagt tatttggcgg tggtggtcat gtaagggctg
    17521 ctggatgtac tattagagat agtattgata atgcaaaaaa aatggtacta gaggcagtgt
    17581 taaaatcaat ttaaggtgac agtaaaatga ataagattat aagtatattg aaacctacag
    17641 gtatgacatc tcatgatgtt gtaagtagag tgagaaagat tttaaatata aaaaaagtcg
    17701 gtcatacagg tactcttgac ccagatgctt caggtgtttt accaatctgt atagggaaag
    17761 ctacaaaggt atgtgaagtt attttaaaca aagataaatc gtatatatgt gagcttacct
    17821 taggtataag tacagatact tatgatgcct caggtgaaat tctaaaaaaa gttgatgatt
    17881 ttaaatttag taatgaagag ataaaaagag cttttgatac acaaagagga gagataaatc
    17941 aacttcctcc aatttattcg gctttaaagg taaatggaaa gagaatgtgt gatttagtaa
    18001 gaagtggtag acaatctgaa atcactttaa aaacaagacg agtaaatata aaaaacataa
    18061 aaatactaag tataaaaggt aataaagtaa tgttttatgt tgagtgttct aaagggactt
    18121 atgtgagaag tatttgccat gatataggag aatatctagg ttgtggagct catatgtcgt
    18181 ttttgaatag aacatcttct ggtaaatttg atttagacaa ttcaataaca ttagaagaat
    18241 tagaattatt ttatgaaaat aaaactttag acaaatactt atatgatata gattatgtac
    18301 ttgattcatt taattatgtt gtattaaact ctaatgctat aaaatattat agcaatggtg
    18361 gttcaataga tgacaaaagg tttttaaaaa ataatttcaa taaagatgat gaatttgtaa
    18421 gagtatacag tacagataat ttcttaggat taggaaagtt gtcaaaacac aataatataa
    18481 taagtgttaa aagtgataag atgttcatat aaataatgta tttaaaaaac aacaaaagag
    18541 atagtactat aataacttct atagtctatc tcttttactt atgctataat agtttgtgaa
    18601 taagttaaga gtaaattata ttttagcggg gaacatttat tatggagatt ataaaatcta
    18661 taaaagaaat agcaaacata aaagaaagtg tcattaccat aggaaatttt gatggtgtac
    18721 acaaaggaca tcaagttctt ataggaaaaa cagttgaaca tgcaaaaaaa gaaaatatac
    18781 agagtattgt atttacattt gcaaatcatc ctgtaaatta ctttagacca aactcaacaa
    18841 aaaatattat aagcaatgat gataaaataa aattattaga agatttggga atagatatag
    18901 ttgtaatcat accatttgat gagtatatga ctaaaatacc tgcaaaggat tttgtagatg
    18961 agatactagt aaaaaaatta aaagctaaaa agatagtaat tggacatgac tttacatttg
    19021 caagggcaaa agaaggcaat gttaatctat taaaaagctt agaacatgaa tttggttttg
    19081 aggtagaagt aataaaacca atcaaaatca atgatgtaag agtgagtagt acttatatta
    19141 gaagtttagt atcccagggt gatatggcta gtgtcaagga atatttggga agaaattaca
    19201 agttagaagg ttgcgttata cattctaaac atcttggaag aactatagga ttcccaacag
    19261 caaatattga ccttaagaat aatatgctcg ttccaaagag aggtatttat gcttccattg
    19321 tacatattgg agatgagact tattttggtg ctacaaatgt aggctataac ccaacagtta
    19381 atggaaaaac tttgtctatt gaaacaaata ttttagagtt tgatagagat atttatggag
    19441 aaaatataat tgttgaattt ttggaaagaa taagagatga aagaaagttt aattcaatag
    19501 atgacttaaa aaatcaattg tataatgata caaattatgt gtatgaaaac tatgtttgca
    19561 aaaacaagta atgtatgata aaatgattaa ggaaaataac cattactcgg catttgaata
    19621 ctccaatcaa tgcttagtaa taggcgtttc taaaaaaata cggaggtaat ctaaatgata
    19681 aacaaatcag aaataataaa agaatatgga agagctgaag gagatactgg ttctccagaa
    19741 gtacaaatag ctttattaac agctagaata aacgagttaa atggacattt aaaaactcat
    19801 aagaaagacc accattcaag aagaggtcta ttaaaaatgg ttggtagaag aagaaactta
    19861 ttagcttact taaaggaaaa agatcttgaa ggatatagag cattaatagc taaattagga
    19921 ttaagaaagt aatttcattc tttataaaaa agcaggtata gtgtatacct gcttttttat
    19981 agttaattca ttataaagta aaatagttaa taataaaagt agtttatatg ttataaaaaa
    20041 ttataaaatg aataatattg ggtaagaata ttaaaatagt atacaaatac ataatcaatt
    20101 taaaaggaaa gataggacaa atatagaaat tttataacag gataattagt tagaataagg
    20161 aggatattat tttgaaaata aagttagtat gtgatagttt atgcgatatt ccagatgaaa
    20221 tatcagaaaa agattatata gagatagttc cacttacagt tatttttaat gatagggaat
    20281 atatagaagg agtagatata aagaaagaag agttttataa aaaagtaaaa gaaattaaac
    20341 aaatacctaa aacttcacaa gctacatata tggagtttaa agaagtattt gataagttca
    20401 tagcagaagg ttatcatata atctgtatga caggggctgc aaacgcatca ggaacttttc
    20461 aaagtgctat gatagctaaa aatgatgtag atgaaaatga aaaaatacat atatttgata
    20521 ctagaaattt atctctaggt agtggacaat atgttataaa ggcatgtgaa cttcttgaag
    20581 aaggactggg atttgaaaaa attatagatg aattagaaaa tactcgaagt agtgtaagat
    20641 tactttttgc accatataca cttgattttt taaagcagag tggaagagtt ccagttgcaa
    20701 ctgctttgat aggaaatatg ttaaatataa agcctatatt tttctttgat aatggggaag
    20761 ctaagttggt aaataaggtt agaggtataa aaaatatagc aagtaaattg gtcgatataa
    20821 ttttagaaat gaatgaaggg tctttagaag gaaaaatcat cacaataggt tgtggagata
    20881 atttacatga ttgtgaaata ttaaaagatg aagtaaataa aagaataaaa gcaagaagcg
    20941 tattatttac tcgaggagga gtttctattt gctctcatac agggccagat atattggcta
    21001 taagttgttc taattagtag taatataagg tttttgcata tatatgtgaa gataatttta
    21061 tattgttaat ttatagtatt tttgataata tagtaaatag agtgatatac gtgtttttta
    21121 ataagaggag gacaagcatt aatgtttgaa cataaaatat ttaaaatgga ttttgcagga
    21181 agagaacttt ctgtagaaat aggaaagatt tgtgaaatgg ctagtggtag ttgtatagtt
    21241 cgatacagtg acagtatggt aatggtaaat acaactaaat cagccaaacc tagagacgga
    21301 atagactttt ttcctttaag tgtggattat gaagaaaaat tatactctgt aggtaagata
    21361 cctggaggtt ttttaaagag agaaggtaag ccttcagaaa aagctatact tacatcaaga
    21421 ttgatagata gaccaataag acctttattc ccaaaaggat ttagaaatga tgttcaggta
    21481 gttgcaacag ttctatcagt agaccaagat tgcacaccag atatagtagc aatgataggt
    21541 tcttcaatag ccctttctat atcagacata ccatttaatg gaccaacagg ttcagtatgt
    21601 attggccttg tagatggtgc atttgttgta aatccaaacg cagagcaaag agaaaaaagt
    21661 tcaatgcatc tagttgtttc aggtacaaag gaagcaataa tgatggttga agcaggagct
    21721 gacgaagttc cagatgaggt aatgcttgat gctattttat ttgctcacca agaaataaaa
    21781 aaaatagttg agtttataga aggtatagtt gcagaagttg gaaaagaaaa aatgcctgtt
    21841 gaattatacc atgcaggaga agaaataact caattggttc gtgaatttgc aactgataag
    21901 atgaaaaaag cagttcagac ttttgaaaag ttagagagaa tggaaaatat ggatagagtt
    21961 aaagaagaaa ctttagctca ctttgaagag actttagaag attttgaaga ttttgttgga
    22021 gatatagaag aagtattaca agatataata aaagaagaag taagaaaact tatagtacat
    22081 gaaaatgtaa gaccagataa tagaaaactt gaagaaataa gacctatatg gtgtgaaact
    22141 ggcatgatac ctagagctca tggttcagcc atatttacta gaggacaaac tcaagtactt
    22201 aatgttgcta ctttaggtgc tttaggagat gttcaaaaat tagatgggtt agatgaagaa
    22261 gaaaacaaga gatatatgca tcattataat ttccctgcat atagtgttgg agaagctaga
    22321 ccatcaagag gaccaggaag aagagaaata ggacatggtg ctctagcaga aagagcttta
    22381 ttaccagtaa taccatctca agaagaattt ccttatgcta ttagattagt ttctgaagta
    22441 ttaagttcaa atggttctac atctcaagca agtgtatgtg gctcaacatt atctttactt
    22501 gatgcaggtg tacctataaa agatatggtt gcaggtatag caatgggact tataaagcat
    22561 gatggtaagg ttgcagtgct ttcagatata caaggtatgg aagaccattt aggtgatatg
    22621 gactttaaag tagctggaac agagtatggt ataacagcaa tacaaatgga tataaaaatt
    22681 gatggtatag ataaagaaat tctacaacgt gctttaaaac aagctaaaga aggtagaatc
    22741 catatactag gagagatgag aaaaactata tctcagccaa aaccagaact ttcaccatat
    22801 gcacctaaga ttgttaaaat gcaaataaat cctgacaaga taaaagatgt aataggacct
    22861 ggtggaaaga taataactaa gataatagac gaaactggag ttaagataga tattgaacaa
    22921 actggggaag tatttatatc aggtattgag attgatatga ttaagaaagc tcaagaactt
    22981 ataaataata tagtagtaga accagaagta ggaaaaactt ataaaggtaa agtaagtaga
    23041 ataatgaact ttggagcatt tgttgagata cttccaggaa aagaaggatt acttcatata
    23101 tctcatatag ctcatgaaag agttgctaaa gttgaagatg tattgaatat aggggatgaa
    23161 gttgaagtta aagttacaga gattgatgaa aaaggaagag ttaatttatc aagaaaagtt
    23221 cttttaccaa aaccagagca taaaaataaa taatttatta aataataaaa agcaaagtct
    23281 taggattttg ctttttatta tttggtgcat aaacagagct tatttttaat agtattaaat
    23341 aagaaacaat ttgtacataa ttagggggag ttttaatgat aatgatggta cttaataaga
    23401 gtaagctcaa aaaaatcata atactaattt tagtagtgtt attagtatta tcaggagggg
    23461 taatggtatt aaagttattt aacaacaagc cagtatttaa tttatttaat ggtgtagatt
    23521 taccatatga aagaggtaca aaggatgaaa gtggatacgt tgcattaacc tgtaatatag
    23581 accttggatg ggaaacagag tacgtagaat ccattttaga aacacttaaa aaagaaaatg
    23641 taaagattac atttaatgta accggaaaat gggcagaaaa gaataaggac gaattattaa
    23701 agataaaaaa acaaggacat gaaataggta atcatggata caaacattta gattactcta
    23761 cattatccta tgaagacaat tatgagcaaa tagagacctc taaaaagata attgaagaaa
    23821 ttataggtga aaaaactaaa ttttttcaag caccagcagg ttcttttggt ccagaaacag
    23881 taaaagctgc taaagcatta gggtatactt ctatcaaatg ggatgccgat actatagact
    23941 ggaaatacaa agaccaacca gaggtaatca ttgatagaat gaagaaaaaa gatattaaag
    24001 atagtagtat aatcttaatg cacccaacca atgcaacgac aaaatgtata gatgatatta
    24061 tagctattgt aagagaaaaa ggactaaaac caggaaagct tagtgatgta tttaagtaga
    24121 caaagtataa atatttggtt aagattgtat aagttgtagt tgaattaatt aaaagactga
    24181 cttataatgt aatacatata aaaaataaat ataattaaaa catagtgttt tcaataatcc
    24241 aatatgaaac aaagaataaa ttgttaggag aatgtaatgt ataagacaaa aatactggaa
    24301 aatgggctta caataattgg tgaagaaata ccatatctaa agtcaataac attaggtatt
    24361 tggataaatg caggctctag gatagaagaa gcacaggtga gtggaacttc tcattttata
    24421 gagcatatga tgtttaaagg aaccaaaaac agaacatcaa aagagattgc tagctcaata
    24481 gataatttgg gtggtcaact aaatgcattt acaagtaaag aatgtacatg ttattatgta
    24541 aaactaatag atgagcatat agatatagga attgatgtat taagtgatat gatactaaat
    24601 tctaaatttg ataaaaatga tatcgataag gaaagactta taatattgga agaacttaag
    24661 atgtatgaag attctccaga tgacttatct tatgatttat tagtggaaaa tatatatgct
    24721 aatgatggat taggaatgaa tataattggg acaaaagaat ctctatacaa tataactaga
    24781 gagtctatgt tagaatactt aaataagtat tatattccaa ataacgctgt tatttctata
    24841 gctggaaact ttaactttga tgatatggta gagaaaatta aatcaaaatt tggacactgg
    24901 gaaaagaaaa atttgagtat agacataagt gaggctaagt ttaatccttg ctttatatca
    24961 aaaaataaag atactgaaca ggtgaactta gctatgtgcc taaaaggtat tccttttgaa
    25021 aatgatgaag aagtctactc aatggcagtg gtaaataata tttttggtgg aagtataagc
    25081 tcaaggcttt ttcagaaaat aagggaagaa aaaggtttag tatattcaat atactcatca
    25141 cagacattat atagaaaatg tggtgaatta ggtatatttg caagtatgag tacagaaaat
    25201 ttacaagatg tgtataattt agtaaaaaaa gaaattgaaa atataagaga aaactatctt
    25261 accgaagaag aaatatctga aagtaaagaa cagttaaaag gcaattatat attggatttg
    25321 gaaagtacta gcagtagaat gatgtctact ggtaaatcta tgttacttag taaaaaagta
    25381 aaaactacag atgaaatact tgaatgtata aataatgttg atattaattc agtaaagaaa
    25441 gttgttgata aggttttcaa tgtcgagaat attggtacat gtatagttgg aagagatgtg
    25501 gaaaaaattc tacacttgaa ttaatattgt aaaaagtaaa tctctggagg tataaaatat
    25561 gaatttatct gaaatagcag gtaaggaaat agttaacttg gtcacaggag aaagattggg
    25621 ggtcattgga gaatgtgacc ttatactaga tgaaactaca ggcggtatat tagcgcttct
    25681 aattccaaaa gaaaaaggat tttttgccag aagaaaagat aagtcatttt tagaagtacc
    25741 atggagaaat gtaaaaaaaa taggtaatga catgataatt atagaatatg agggatatat
    25801 ttaggaggaa aaaatgagta ttttagtgca aaagtttggt gggacatcag tagaatcata
    25861 tgaaaaaatg aatgaagtat gtaagatagt aaaagcatac aaaaaaaatg atgaagaatt
    25921 acaattagta ctagtagttt cagcaatggg aagaaaagga gctccatacg ctacagatac
    25981 acttataaat ttatgtagtg cagtaaatga tgagccaagc aaaagagaac ttgatttaat
    26041 aatgtcttgt ggggaaataa tatcaggaac aattcttgct aatttattga atgcacaggg
    26101 tatagattct gttttcttta ctggtcagca agcaggtata attacaagtg atgaatattc
    26161 taatgcaaag ataaagtata taaatcctaa aaagataaaa agagctttag atgatgggaa
    26221 ggttgtagtt atagctggat tccagggggt aacagatgat ggagaaataa ctacccttgg
    26281 aagaggagga agcgatacat cagcagttgc tattggtaaa gctttagaat gtgaaacagt
    26341 tgaaatttac actgatgtgg atggtataat gactgcagac cctagggtgg aaccaaacgc
    26401 taaagtattg agttttatag attatgagga agtattccaa atggctgaca aaggtgcaaa
    26461 ggtaatacat ccaagagcag tgcaattagc taaaagtgga aatataactt tggctataaa
    26521 aaatacaatg aacccaactt ttgaaggtac aaaaataggt tctttatgta gacacttaga
    26581 agataatata gaatatgaac aagaaagaga ttttaaagtt gctgttgcaa ataaagatag
    26641 tgtagcacaa gttaagatta agtcagcaga agaagtgttt acagaagtat taaatgagat
    26701 agaaaagaaa tttataacta tagatatgat aaatttcttc atatcagaaa aagcatttgt
    26761 tgtagaagat gctgatataa aatcattgaa ggaaatacta gataaatttg aattggatta
    26821 tgaagtaaat agagactgtg ctaaggtaac attgatatgt tcaagaattg ctgatgaaat
    26881 gtctggtatc atgtcaaagg tagttagagg attatcaaaa gcaggggtat ctcttttaca
    26941 aacatcagac tcaaatatga ctatatcttg tttagttagt gaagaagata tgcatacagc
    27001 agtacatgct atacaccaac aattttattt aaaataaaca atataataca attatttttt
    27061 aatcaaattt gataatttca tatatatctt aatatatttg atttatttgg taaaactaaa
    27121 ttaaatatga ttttgatagg agtttttttt atgaaagatt atagagaaga taatgaaaga
    27181 tttgataaag ataattgtag tgaagaatct agtagtgaag agagagaatg tataaagcaa
    27241 tttggtacca atgaaatgcc acctcaacct cctaaggata ttcaatgtat aactataatt
    27301 ggagaaattg agggacattt tataggaaat cctcaaaaaa aagctacaaa gtatgaacat
    27361 ataattccaa tgctgtactc aattgaggaa agtaatgatg ttaaaggagt attagttgtt
    27421 ttaaacactg ttggaggaga tattgaagca ggtttagcta ttgcagaact tttaaacagt
    27481 acatctaaaa aagtagttac tcttgtttta ggaggaagtc atagtatagg agttcctctt
    27541 gctacagctg gagattactc atttatagca ccaacagcaa ccatgattat acatccagtg
    27601 agaactactg gtcttgtaat tggcataaat gagacttttg aatattttaa aaagatgcaa
    27661 gatagaataa ttcaatttat aataagaaca tcaaatatca aaaaagatgt acttgaaaaa
    27721 cttatgcatg aaaaagatga acttgtaagt gatgttggta gtgtattaat aggaaaagaa
    27781 gctgtggatt atggtcttat agatgaagtt ggaggactaa aagaagcact taaaaaatta
    27841 agagagctta taaaagaatc tgaggaagaa aaaaataatg attaaaattt gtttaaataa
    27901 taaaatttat gatataatga tgtaataaag taggagtgtt agtgttaagg cattagcact
    27961 ttttgctgtg aaggggtaaa acttgctaaa aataaggagt gatattaatg gccacgaaaa
    28021 agaagaaaaa aaagaaggta aataccttaa gttttaatgc agaatatcat aatttaataa
    28081 caatatttat aggtgtattt ttattatata gtttaaattc taattctatg ggatggatac
    28141 ctgtgttaat gcaaaatctt tttaagggac tatttggagg gctttcaata gcaataccat
    28201 ttatagtaat tataacagga cttttaggat tttttgatgg aaatgaatat atatataggc
    28261 taagaaagac taaattatac tacataatta ttttatttat atttgtattt tatggattat
    28321 taaatgcagg tactcttcct gtagatagcc cactaaaagg taacatgttt gatgatgtta
    28381 tgaaattggg tgtaagtgga caaggtagtg gcttgatagc aacaactatt gcgtattata
    28441 tgagtaaaat atttggtata gtaggtggat ggttaataag tatctttgca cttatacttt
    28501 ctgtaatgtt tatatttaat atttctataa aagatatgat atcaaatgca aaatcaaaat
    28561 ctaaagcatc aaaagacagc aatttaacgt ttaaagataa aattgcaaat atgaaaaaat
    28621 ctgcaataga tatgatgact gatgaagtag atgatactac tataaataaa aaaccaggtt
    28681 tctttaaagg gttaatgtca aaaggtcgta atactgatga agaagatgaa tattttgaag
    28741 aagaaaatag tgatggtgta gatgataaga ctataaaaat agttggattt aataaggcag
    28801 aagatgagta tttagagata ttagaaggaa ctcaaagtat gccagagtta gatgttttaa
    28861 aagaattaca aaaagctaca aatgaaaatc cagttataga cactaaacca gaaaaaaaag
    28921 ttgatatagc taagcctaac ttaaatatag aaaaaactca acctatgagt attgtagcag
    28981 agccagttaa tgaggattat tcaaactata agaagcctag cattgagctc ttaaataaag
    29041 taaataagaa gtctgatgaa aatggtaaaa agaaagtgtt aaaaaatgct tctcttttag
    29101 aaaagacact atcagatttt ggagtggaag caaaaattaa tcaagttaca gttggaccta
    29161 ctataacaag gtatgaaata caaccaagtc caggagtcaa ggtaagtaaa atcgtaaatt
    29221 taactgatga tatagcttta agtttagcag ctaaaagtat aagaattgaa gcaccaattc
    29281 ctggtaaaag tgcaattgga atagaagttc caaatgaaga agctcaaatg gttggtgtga
    29341 gagaagttct agaaagtgaa gaatttaata attttaactc tcctcttgca atgggacttg
    29401 gaaaagatgt agcaggaaaa attataattg gagatatagg taagatgcca cacttactaa
    29461 tagctggttc tacaggttca ggaaagagtg tatgtgtaaa tacattaata agtagtattt
    29521 tatataaggc aaatcctgat gaagtaaaat tactattaat agacccaaaa gtagttgaac
    29581 ttgcaaatta taatgggata cctcatctgt tgataccagt agttactgac cctaaaaaag
    29641 ctgcaaatgc tttaaactgg gcagttacag agatgaatag aaggtacaag ctatttgcag
    29701 atgctcaagt aaaagatgta acaagttata atgagaaagc agaagaaaaa cttcctaaaa
    29761 tagtaataat aatagatgag ttagcagatt taatgatggc aagtgcaaat gatgtagaag
    29821 attatatttg tagactagct caaatggcaa gagctgctgg aatgcacttg attgtagcaa
    29881 ctcaaagacc atctgttgat gttataacag gggtaataaa ggctaatata ccatctagaa
    29941 tagcatttgc agtatcatct caaactgatt caagaacaat ccttgatatg ggtggtgcag
    30001 agaagcttct aggtaaaggt gatatgttat tctacccact tggtgcagca aagccagtta
    30061 gacttcaagg tgcattcata tcagaaagtg agtcggaaaa agtaattgat tttgtgaaaa
    30121 gtcaggttaa agatggaata aaatatgaag aagatataat tgaaactata tctaaggtta
    30181 atacttcaaa gggtagcgat gaggatgagt ttttgagtga agcgatagaa tttgttgtgg
    30241 aaagtggtca agcatctgcc tctatgttac agagaagatt taaaatagga tttaatagag
    30301 cagctagact tatagactct atggaagaaa gaggaataat tggagcaagt gagggaagta
    30361 aaccgagaaa ggtattaata agcaaacagg atttgcagaa tttagaaggt gaatagaatt
    30421 gtcagttata aaaatagaaa aagcacttaa gctagacaaa gtaatatttg tagatgtaag
    30481 aactgaaggg gaatatgaag aagaccatat attgaatgca attaatatgc ctctttttaa
    30541 aaataatgaa cataatgaag ttggaacaat ttataaaatg cagggaaaac atgaagcaat
    30601 acaaaagggg tttgactatg tatcttataa attaaaggat atatatttac aagcagcaga
    30661 acttgcttta aattatgata atattgtcat atattgtgca agaggtggaa tgagaagtgg
    30721 aagtatagta aatttattat catcattagg tgtaaatgta tatcaacttg aaggtggata
    30781 taaggcttat agaaattttg ttttagagta tttaaggcat gtaatggata caaaaaactt
    30841 tgtatctgtt catggactta ctggtacagg aaagactgat ttactacatt tattagatga
    30901 aaaaggaata gatgtactag atttagaagg catagctaaa aacagtggtt ctacctttgg
    30961 ttttataacg tttgataaaa aaccaccatc tcaaaagaac tttgaaacaa aattatttga
    31021 aagtatattt ttctcaaaag agaattatat ctttgtcgag agtgaaagta aacgagttgg
    31081 aagtgttaca attccaaatg aaattttcga ggcaatgaca cgagaaggac atcatatatt
    31141 attagaatgt gatattgaaa atagagttga tagactttgt agagattata tttatgataa
    31201 agatgaaggt aatctattgg ttttaaaaga atgtataaac aaatttagaa aaagattggg
    31261 tcataaagta gtagatgatt atattgattt attagaatca ggtgaatata aagaactcgt
    31321 aaaaagatat cttttagaat actacgaccc actttatatg cattcagtag agaaatataa
    31381 gtataataag gttataaact ttaatgatac agaagaagct ttagaagatg ttatagactt
    31441 atatgagagt atattggaag gagaaaatga atgttaaaaa tagcattaga atcattagga
    31501 tgctcaaaaa atttagtaga tgcagaaata atgatgggaa ttttaaataa caaaggctat
    31561 aaattaattg gagattttga agaagctgat gtaatcatag taaatacatg tggatttata
    31621 gagtcagcta aacaagaatc tatagatacg ataataaact ttgccgaatt aaaaaaaact
    31681 ggcaatttaa agttgttgat agtaacaggg tgtttagccc aaagatattc tgaagaatta
    31741 aaaactgaaa ttcctgaaat agatgctata gtaggtacag gaagctatca aaatatagat
    31801 aaaatactta gagaattaag tgaaatgcat caaattgtaa gccttaatga tatagagttt
    31861 gtatttaatg aagatttacc aagatatcta tctacaccta gctatatggc atatttaaaa
    31921 attggagaag gatgctccaa taattgtact tattgtataa taccaaaact aagaggtaaa
    31981 tatagaagta gaaaatttga agatataatt aaagaagcta agaaattagc tgaatctgga
    32041 gtaaaagaac ttgtagttat agcacaagat acgacaaagt atggttttga tttatatgga
    32101 aaagaaagat tatcagaact tttagaagaa cttgcaaaga tagatggatt taagtggata
    32161 agagttatgt actcatatcc agaatctata acagaagaat taatacaagt tataaagaaa
    32221 tatgataata tatgtagtta ctttgatatg ccaatacaac atgcaagtaa caatatttta
    32281 aaattaatga atagaaaaac tactaaagaa gatatattaa ataaaatcaa tcttataaga
    32341 agtaatatac cagatgctat acttagaact actataatag taggatttcc tggcgaaaca
    32401 gaagatgact ttaaacaact tgtggatttt gttgaagagg ttaaatttga tagacttggt
    32461 gcatttgcat attcaagaga agaagataca ccagcagata gattgccaaa ccatatagat
    32521 gaagacgtaa aaatacaaag aagagatact ttaatgatga ttcaacaaaa aatatctgaa
    32581 gaacttaatg acaaaaagat aggaaaaact tatgaggtgt taatagaaga acagatagaa
    32641 gataatgtat atacaggaag aacacaaggt gatgcggaag aaattgacag tatagtgtat
    32701 gtgaaaagtg tagacaatct tgaaatagga gaatttgtaa gtgttaaaat aaatgatgca
    32761 atggaatacg atttaatggg agatgtttta tatgaacttg cctaacaagt taactttgtt
    32821 tagaatattt ttaataccag tatttgtctt aattatgttg ctcaatatac caaataaatt
    32881 tttaatagct tgtattattt ttataatagc ttcaattact gatgctttag atggaaaaat
    32941 agctagaaaa tacaatctag tcactgactt tggaaaattt atggatccat tagcagataa
    33001 gctattagtt atttcagcac ttacatgtat gatagaagac catcttgtaa gcagttggat
    33061 ggtaattata atagtggcta gagagcttac agttagcata ttaagagcta tagctgctgc
    33121 tgatggaaaa gtaattgcag ctggaaacag tggaaaactt aaaacgataa ctcaaatggt
    33181 atctatagtg cttttattgt taggtgctca gtttgcaaat ggattgatat taaatattgg
    33241 tgaaattctc attcttatag ctactctact tacattatat tctggatggg aatatttata
    33301 taaaaataaa gagctcttta tgtcttctaa gtaaaaaatt atgctttaat aaataaaaat
    33361 taacaactta aaattttaaa aatgta
//
LOCUS       DAFNNB010000026        32962 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0026, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000026 DAFNNB010000000
VERSION     DAFNNB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 32962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0026"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(151..1293)
                     /locus_tag="L3M60_001084"
     CDS             complement(151..1293)
                     /locus_tag="L3M60_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBN6225773.1"
                     /translation="MRYVEYGKTGKMVSVVGYGGLRFDLEKSNQENADLVKYAYEKGI
                     NYFDTAPGYCDDRSEDIFGLAFRQMIKEGKTDFYVSTKEKPKACDTSEKAIEAVKKSI
                     ERLGVSKINFYHVWNIRKMEHYELAMRPGGQYEGLLKCKEEGLIDHIVFSSHQPGDEV
                     IDILSENKFEGVTMGINILNFPYRLKGAKYAVDNGYGVVAMNPLGGGTIPKYNKELSF
                     LSKDDESTIESALRFNIGISQISISLIGFNKKQDIDDACKIADENRIYSDEDIHAIET
                     RLNKNMNEICTGCGYCKVCPKGINTPAYMLFYNEKQMFKKSDEEMTKLVYGLGHWNYT
                     MNSKAKAKECISCGKCEVECTQHLPIIDRLKEIKKWEEDGANTVKV"
     gene            complement(1434..2300)
                     /locus_tag="L3M60_001085"
     CDS             complement(1434..2300)
                     /locus_tag="L3M60_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBN6225774.1"
                     /translation="MDNYKKVKQVLWIILFANFAVALLKIIIGNQIKSYSMTADGFHS
                     LSDGASNIVGLIGIFFASKPKDKNHPYGHKKFEIITSLFISGMLFVIAIKIILSAVSR
                     IANPVVPAITIESLIALIITLFINIFVCMYEYRVGTKLNSYVLISDSLHTRSDIFVSL
                     GVLVTLFGVKLGFPVIIESIVPIIISVFIIYSAYGIFRPSIGILVDRVAVDEDYIKEI
                     VFEFNEVRDVHNIRSRGSKSSIYIDMHVMVDPFISVEKSHDLTHKIEKQIQEEINENA
                     QVIVHIEPFYSF"
     gene            complement(2578..4125)
                     /locus_tag="L3M60_001086"
     CDS             complement(2578..4125)
                     /locus_tag="L3M60_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBN6225775.1"
                     /translation="MANFFNFILLQKDKIIELLIQHMSLTVTSILIAIIVGVPLGIII
                     SRISSLRKLVLGFVNLVQAVPSMALLGLLVPILGIGSKPAIFMVVVYSLLPIVKNTYI
                     GITSIDPVVLESAKGIGLTRNQTLFKIQFPLALPIIMGGVRISAVTAVGLMTLAAFIG
                     AGGLGYLVFSGVQTVNNNMILAGAIPSCIIALLVDYLFSKIEVAVTPKGLNPKAPKKN
                     YIALKVISVVVVVSMLFVVFSSSFSSKKDTITIGSKDYTEQLILGNVYAELVEKNTNL
                     KVNKNLNLGGSSVAFNAIKSGELDMYVDYTGTLLVNVMRHTPIKDADEAYNVVKDTME
                     KENQLTLLDPLGFNNTYTLAMMPETAEKYGINTISDLTKYGKEFTFSPTLEFENREDG
                     LVGLSRDYGLKFKDVKAMNGSLRYTALDNNESQVIDAFLTDGLIKKFNLKILEDDKNF
                     FVPYYAAPLVREDTLKKYPELEKVLNMLSGKINEETMRELNYQVDELGKSPEDVAHSF
                     LVKEGLV"
     gene            complement(4118..5254)
                     /locus_tag="L3M60_001087"
     CDS             complement(4118..5254)
                     /locus_tag="L3M60_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225776.1"
                     /translation="MIEIRNVTKKIGNNVILDDISLVVETGTLVVLIGSSGCGKTTTL
                     KLINKLIKPTSGEIYINGKPISQENEIELRRKIGYVIQNTGLFPHLTIKENIELIPRL
                     KKEKSVEEIEKRTLQLLEMVGLDSGEFLNKYPSELSGGQQQRIGVARAIATDAEIILM
                     DEPFSALDPITRTSLQEQLFSLQDELKKTIIFVTHDMDEALKIADKICIMKDGRIAQY
                     DTPENILRKPANDFVKDFIGEDRVWDNPEYIKARDIMIKNPIAVNSTRTVTQGIEIMR
                     TSKVDSLLIIDRAKTLKGIVTVKDMKDVDDKSILLADIMSSEPLHVNEGDNLVEILNV
                     MNRNSVGYIPVISDENKLVGLITRSSLLSVLSEQFLEMEVSVLG"
     gene            complement(5517..6614)
                     /gene="dinB"
                     /locus_tag="L3M60_001088"
     CDS             complement(5517..6614)
                     /gene="dinB"
                     /locus_tag="L3M60_001088"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBN6225777.1"
                     /translation="MELHRKIIHIDMDAFYASIEQRDNKKLKGRPVIVGGNPQSRGVV
                     ATCSYEARKFGIHSAMPSAVAYNRCPYAVFVRPRFDVYKSVSEQIRDIFYRYTDLVEP
                     LSLDEAYLDVTKNKKNIDSSIEIAKQIKKDIFREVGLTSSAGVSYNKFLAKIASDLKK
                     PNGLTVITEENAQDFLDKLPVNKFFGVGKVTSNTLKNLGIKTGYDLRCLNLFELENIF
                     KKRGYELYKFARGIDDRPVEPNRVRKSVGAETTLSHNLNIDEEETRNILDELCEEVCH
                     RLKSSEKFGKTLTLKIKYEDFTKITRSLSLEHYIDEYNDIRRGIDNLLRNVEMNGKQI
                     RLLGVTISNLSDKKETYKDITLFEYMDSIQM"
     gene            complement(7010..8515)
                     /locus_tag="L3M60_001089"
     CDS             complement(7010..8515)
                     /locus_tag="L3M60_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HBN6225778.1"
                     /translation="MKVGFDHKKYLEEQSKYILERVNNFDKLYLEFGGKLMGDLHAKR
                     VLPGFDENAKIKVLQHMKEKVEVVICVYAGDIERNKIRGDFGITYDMEVLRLIDDLRG
                     YELDVNSVVITRFEGQPATTVFINKLERRGIKVYKHYPTKGYPSDVETIVSDEGYGAN
                     EYIETTKPIVVVTAPGPNSGKLGTCLSQLYHEHKRGNVVGYSKFETFPVWNVPVKHPL
                     NIAYEAATVDLKDVNMIDSFHLETYGEMSVNYNRDLELFPVLKKIIEKITGKESIFQS
                     PTDMGVNRVGFGIIDDEVIRKASIEEIIRRYFKTACEYKKGQVDKGAYDRMKMIMEEL
                     NLKPEDRNVVLPARNYSNKLKESADKNDTCPVVALELEDGTILTGKGSDLMNGTAAAV
                     LNAIKHLANISDDMHLISPVILEPIINLKTKTLASRNVSLSCQEVLTALSICAVTNPT
                     AQFAMEKLALLKGCQAHSTTILNRDDEQTFRKLGIDVTCDPEYPSQNLYYS"
     gene            complement(8776..9126)
                     /locus_tag="L3M60_001090"
     CDS             complement(8776..9126)
                     /locus_tag="L3M60_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225779.1"
                     /translation="MAIVDKIFNEIKLMVARLLLGKKYSEYEEKDLVYNTEEEVILIT
                     LKRLVFQGKVNEAEEILFDKAKTVNSENMQYIAIEFYTMLMEKTDEELEAMNFSKQEV
                     YQGIEDIRKVLNLE"
     gene            complement(9348..9539)
                     /locus_tag="L3M60_001091"
     CDS             complement(9348..9539)
                     /locus_tag="L3M60_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225780.1"
                     /translation="MLDRFFGEGGFGGCAWWIIILFFLFLAFQDCWAEMDLCAWIPFL
                     ILLLILCECGDVFDGGCGC"
     gene            complement(9657..10571)
                     /locus_tag="L3M60_001092"
     CDS             complement(9657..10571)
                     /locus_tag="L3M60_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6225781.1"
                     /translation="MKKIILKDILFLVTTLSLIVILLIVVNITANGKIKSTIKSYLKS
                     DKNSVSQNDIQSSTKNTEFKTTILNTGKSDCILVEIGDKVIMIDSGEDKNGKQIVDRL
                     KEKGINTLDYLILTHLDKDHIGGVDSVLSSVKVKNIIQANYKKDSKQYDEYIDSLKKA
                     DIEPVLLKENMNIVINSAEINIQSALKSKYESSNDYSIITSISYGAHKFLFAGDAEEK
                     RLSEFINGNTLKYDFVKIPHHGRYDKLTEAFLESISPKYAVITCSEKKEPDEEILKIL
                     EKLNIKTFLTSNGEVVINSDGKTLNVNQ"
     gene            complement(10705..12201)
                     /locus_tag="L3M60_001093"
     CDS             complement(10705..12201)
                     /locus_tag="L3M60_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6225782.1"
                     /translation="MFEFETQLKRLKHEVLTAVAKLSIEENLTKENIQKIPYEIIKGE
                     KARYRCCVYKEREVVLERAQLALSLTPNSKYGDINPDSVGLDTDEQIIYIVEAACDRC
                     PINEFTVTELCRGCLAHRCKEACKFGAISYINGRAYINHDLCKACGMCKSSCQYDAIS
                     EVVRPCKSVCPTGALDFNRNTMKAMIHEDNCINCGACMSACPFGAISDKSLIAPVAKK
                     LAKKENMYAIVAPAITGQIDSNITYGQLKNAIKSLGFIDMIEASCGADAVTVHESNEL
                     IERIKSGDNYMTNSCCPGFFKYIEKIFPDEKDRISSTVSPMVATGRLVKKIDSDAKVV
                     FIGPCTAKKSEATEKCLKDSVDYVLTFEELMALFEAFNIDLSKCSNEDINDGSIFGRG
                     FGASGGLAKSIQNYIAEKGLSFDFEPIKVSGPRQIKKTMTMAKLGRLEGNFIEGMMCE
                     EGCINGAGKFLSGVKAKNTFDRKNGQSTKKSVLSNDKIKEFEGVNLER"
     gene            complement(12481..13971)
                     /locus_tag="L3M60_001094"
     CDS             complement(12481..13971)
                     /locus_tag="L3M60_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6225783.1"
                     /translation="MFQFETQIHKLRHDVLTAIARLGKEDNLTREAMDNIQYEIIKGE
                     KPTYRCCVYKERAIISERVNVSMGLQPGQDKIDLDTMDIEENEQIIYILEAACDSCPI
                     NEFTVTDICRGCLAHRCKEACKFGAITHVGGMAYINHELCKACGMCKKACQYDAISEV
                     VRPCKSVCPTNALGFDRENMKAMIHEEKCLNCGACMSACPFGAISDKSLIAPVARKLV
                     QKEKMYAVVAPAITGQVEANVTYGQIKNAIKSLGFVDVLEAACGADAVTVHESLEFAE
                     RMENGEDFMTNSCCPGFVGYIEKTYPNHVGKISSTVSPMIASGRFIKSHEPDAKVVFI
                     GPCTAKKSEAAKKELKGAVDYVLTFEEIMALFEAFDIDLAKCSNEDIDDASIFGRGFG
                     AAGGLTKAIENYLAEKGIGVNFEPVKISGSREIKKTMTMANLGKLEGNFIEGMMCEGG
                     CINGSGKFLSGAKVKATFDRKNSQSTKKSVLANTKIKDFSNINLEK"
     gene            14438..14638
                     /locus_tag="L3M60_001095"
     CDS             14438..14638
                     /locus_tag="L3M60_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBN6225784.1"
                     /translation="MKNGIVKWFNNEKGFGFISVEGEDDVFVHFSAIQNDGYKTLEEG
                     EKVSFDITQGNRGPQAENVNRI"
     gene            complement(14818..15696)
                     /gene="speB"
                     /locus_tag="L3M60_001096"
     CDS             complement(14818..15696)
                     /gene="speB"
                     /locus_tag="L3M60_001096"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBN6225785.1"
                     /translation="MKNNFYHMNTFMSMDKNYEESNLIVFGVGFDGTTSNRPGARFAS
                     SSMRKEFYGLETYSPFLDLDLEDYNICDYGDLEISVGSTEQVLKEIYQETYKIVRDSK
                     VPFMIGGEHLVTLPAFKAVYEKYNDIYVIHFDAHTDLREEYNNSKNSHATVIKRIWDI
                     VGDNKIFQFGIRSGTKEEFKFATEEKHTYMEIGGIDTFEKIVNMLNGKNIYITIDLDV
                     LDASVFPGTGTPEPGGVNYREFQEIFKIIKNSNINIVGCDIVELSPDYDTTGVSTVIA
                     CKILRELCLIISEKIK"
     gene            complement(15686..16537)
                     /gene="speE"
                     /locus_tag="L3M60_001097"
     CDS             complement(15686..16537)
                     /gene="speE"
                     /locus_tag="L3M60_001097"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBN6225786.1"
                     /translation="MELWYTEEWTENVRFSIKVNKHLFEGKSQFQRIDVFDSDEFGKF
                     LTIDGLMMVTYKDEFIYHEMITHVPMATNLNIKKVLVIGGGDGGTVRELSRYPQIEKI
                     DMVEIDKMVVDVSKKYMDICSCKLDDERVNLYFEDGVNFVKCAHDKSYDLIIVDSTDP
                     IGPGEGLFSTDFYKDCYRILTDDGILVNQSESPYFDFNAKEMKRANKKLKQIFPISEV
                     YQAHIPTYPSGHWLFGFASKKLNPVKNQDRNGWEKLSLKTKYYNSDIHLGSFMLPQYV
                     KEMLDEE"
     gene            complement(16567..16980)
                     /gene="speD"
                     /locus_tag="L3M60_001098"
     CDS             complement(16567..16980)
                     /gene="speD"
                     /locus_tag="L3M60_001098"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBN6225787.1"
                     /translation="MKFETLGRHILVEYYNCDENVLKDPRLIENFMNDSALNAKATIV
                     DSVFHHFNPWGVSGAVIIAESHLTIHTWPEYGYAAADFFTCGDIDPWKSFELLEQLLK
                     SERSESTEIPRGLTTKIKKYSKKDLGKITHKPEAV"
     gene            complement(17067..18542)
                     /locus_tag="L3M60_001099"
     CDS             complement(17067..18542)
                     /locus_tag="L3M60_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HBN6225788.1"
                     /translation="MDSEFANAVELSYECAPLLESLKEYSEKDIACFDVPGHVKNRGV
                     AILNKYLGESLMKMDINSSPTMDNVSAPNGIIKNAQDLLAQAYMADEAFFITNGTTQA
                     IHAMILSVIKPGEKVLLPRNIHKSVINALILCGGIPIFIQPEFDEKLGISLNITFEKV
                     RKEIEKDCNIKALFFLNPTYYGVCADLESIIELCHKNNVLVLVDEAHGAHFPFHLDLP
                     PSAISLGADMVAVSIHKTGGALTQSSALLLNRDNVSFEKVLQSINMLQSTSASYLLMA
                     SIDGARVNLVENGEKQLSKALNLSRYAKSKLNKIDGIKVLSTEILKQKGVKFIDETKL
                     CINVKELNLTGFEVYDLLYKNFSIQVELGDSYNILALVSIGTNKSDIDRLVKALSIIA
                     KIYRKESTLNEFNMVQINPIIKLNPREAFYAPKESVEINSCIDRICGESIMAYPPGIP
                     IIAPGELITEEIMEYIIFLKNSNAYLTDVQDKNLDRILVIK"
     gene            complement(19195..19848)
                     /locus_tag="L3M60_001100"
     CDS             complement(19195..19848)
                     /locus_tag="L3M60_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6225789.1"
                     /translation="MEERLMKVDPLTTQVYDYISKKIQNGEYEANQRITESEICKSIG
                     VSRTPAREALTRLAGENLLEKIPNKGFVVKEFQEKEKLDTYSVIGVLDALAGSSALQN
                     LTESDLVKMEELIEMMAVSIKYKNYSSYLKLSNEFHDIYITKSNNQVLVNLLNSLRYN
                     FMSKSYTSDNEDELYKMLTYSNNQHIEVVKFMRENDLENIERVLREHWKTIPIAEMK"
     gene            complement(20079..21944)
                     /locus_tag="L3M60_001101"
     CDS             complement(20079..21944)
                     /locus_tag="L3M60_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HBN6225790.1"
                     /translation="MQSVIEYNSWDKNFKAPFGALKFDEELNICVKVNEGYNVKSIFL
                     EINREEEMRTITLNEELDNDKLGKCFCGKIEKFDGTGVYFYYFKVDVEMDGQIKTLFY
                     GKNRDNGYSCEYNYSDINKYQITVYKDFKVPTWYKEGILYHIFVDRFNNGNRSGKVDN
                     PKKNSFIYGNWEDIPMYIKDSQGDVIRWDFHGGNLRGIINKLGYLKKLGVSILYLSPI
                     FEASSNHKYDTGDYKKIDPMFGDEDTFKELINKAKEKGISIVLDGVFSHTGADSKYFN
                     MYGNYNSLGAYQSKESPYYSWYMFEEFPQKYKSWWDVKTLPNINELEHSYMDYIIYDK
                     DSVINKWINMGIKGWRLDVADELPTKFIRELKKELKKADDDSILIGEVWEDASNKISY
                     GQRRSYLLGEELDSVMGYPFRNNMFSFLKGEINSYELCNRYMQIKENYPKESFKSNLN
                     LIGTHDVTRAKTELNDDVDLVKLAVAIQMTFEGVPYIYYGDEAGLCGGVDPDNRRTYP
                     WKNEDEDMLNFYKDVIKIRNKNKLLSSGNTEFIYTNNDSVFSFIRVNENNDRILILVN
                     RSENVESISLCMDSSFIEEIPIKYSLKNVNSTIQIENNELKVDLESKSFRIFRVN"
     gene            complement(21949..24369)
                     /locus_tag="L3M60_001102"
     CDS             complement(21949..24369)
                     /locus_tag="L3M60_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HBN6225791.1"
                     /translation="MVTISKKKFKQKFEVKMYSLYAQSIKEATDEQLLNVLCSLLKDE
                     IAKKWVATKLDKKKEVYYFSLEFLIGRQLKSNLLNLDIEEEVREGLSELGINLDDLIE
                     AEVDPALGNGGLGRLAACFLDSMASLNISGQGYGIRYKYGLFEQKFINGYQVEVPDNW
                     LTEGRYAWETVRPNEATMVKFGGEVELIKEGSHLKVIHKNYLPVMAMPYDIPIIGYQN
                     QCINTLRLFKSEIPKRDFGELTSNALNYSGSYEEALKHKYYTEEISQVLYPDDSNYAG
                     KLLRLKQEYFFVSAGIQDIIRKYKKNKLNINNLFDKVAIHINDTHPTLCIPELMRILL
                     DEENLSWDEAWEITKKTVSYTNHTIMSEAMEKWPVSMMKELLPRIYMIIEEINRRYVE
                     ELNNKGYDQDRVKRMSIIDYDNINMANLCIVTSHSVNGVAKLHTHILETEVLKDFYQD
                     EPNKFNNKTNGIAHRRWLISSNPQLSNLITDLIGDSWKRDTLQLKNIEKFKNDSSVLQ
                     RLDDIKYNNKANLAKFIKDKYDLNIDPSSIFDVQVKRLHAYKRQLLNIFNVLHMYHEL
                     LDNPNLNLDPRTFIFGAKAAPGYYLAKCIIKFINSVASTINNDVRVKDKLKVVFLENY
                     GVSLAEIIIPAANVSEQISTTTKEASGTSNMKFMMNGAITLATLDGANVEICEQVGKE
                     NMFLFGLSAEQVLNYNKYGGYSSLDLYHSNMDIKRVVDDLINGFIPNLGEEGRSIYNS
                     LTTYNDEYFVLRDFENYGQAQADINRLYRDKEKWNKMSLVNIANSGFFSSDRTISEYA
                     KDIWFKRV"
     gene            complement(24383..25825)
                     /gene="glgA"
                     /locus_tag="L3M60_001103"
     CDS             complement(24383..25825)
                     /gene="glgA"
                     /locus_tag="L3M60_001103"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011053260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBN6225792.1"
                     /translation="MKVFYVTAECWPFAKTGGLGDVSYALPKELKKEGVDVRVIMPKY
                     STIPSYLKDQLKEIAVFSVRVGWRNQYCGLLEMELDGVKFYFIDNEFYFRREDERKSI
                     YGYGDDAERYTFFTNAVLESISRIDFYPDVIHINDWHTGMLPLILKERYATLEGYKNI
                     KTMYTIHNLQYQGVFDKHVLYDILDLPQTYFDNGDIEYYGSINFMKAGINFADKIITV
                     SPTYANEIQTSFYGEQLDGLLRKESGKLKGILNGIDYDLNDPAKDKDIFVHYDVDSID
                     KKVENKLRLQDILGLKKDASIPLIGIVSRLVSQKGFDLIAYMMPELMREDLQIVVLGT
                     GEHQYQSMFNYYDSNFSDKVSARITFNASLAQQIYAASDMFLMPSLFEPCGIGQMLAM
                     RYGSLPIVRETGGLRDTVTPYNKFTGEGNGFSFKNYNAHEMFFCLKNAIKVFKDKEKW
                     IKLVENAMKTDNSWKKSAKEYIETYRDICD"
     gene            complement(25854..26984)
                     /gene="glgD"
                     /locus_tag="L3M60_001104"
     CDS             complement(25854..26984)
                     /gene="glgD"
                     /locus_tag="L3M60_001104"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HBN6225793.1"
                     /translation="MRNECLGIINLNKKGDPAINKLNYGRPIASTPIAGRYRIIDFAL
                     SNMINSGITKVGIFAKEKYRSLTDHIGSGKDWDLSRKKGGLSIFSPENTKYRNTYSHR
                     EGDIYTILANLDYIEKSEEEYILIAPSYMLCNLNYSQALEYHKKSHNDITIIYKNVNN
                     ANKDFAGNLTLNLDSNNRVINVGNNLGKFPRANICMETYIMKREDFVECIYNIVNKGN
                     YCYLEEFIIEEAENMKIGAYEYTGYLKCVNSVESYFEMSKDLLNLEVADELLYSERKI
                     FTKEKNESPTIYTDSAKVENSFIASGCLIEGTVKDSIIFRKVNVEKGTVIENSIVMQN
                     CVIKSNAKLYNAILDKNTSVSNGKELRGDEKYPLVVEKNTNI"
     gene            complement(26986..28146)
                     /locus_tag="L3M60_001105"
     CDS             complement(26986..28146)
                     /locus_tag="L3M60_001105"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBN6225794.1"
                     /translation="MKKEMLAMILAGGQGSRLGVFTKRIAKPAVSFGGKYRIIDFVLS
                     NCSNSGIDTVGVLTQYRPLILNSHIGMGSHWDLDRINGGVYVLQPFMNEKEGNWYNGT
                     AHAIYQNMDFVDTYNPEYVLILSGDHIYKMDYSKMLKFHKEKGSKATIAVIEVPWDEA
                     SRFGIMNTNEDSSIYEFEEKPSEPKSNLASMGVYIFDWKMLRNYFKEAEKNPEINYDD
                     FGKNLIPKMLEDNVGMYAYPFKGYWRDVGTIQSLWDANMDIIKSPETLDLADPKWKIY
                     TNTMAMPPQYIGKNANVHRSMIADGCRILGEVGNSVLSHGVVVGKGSKVIDSVIMPNV
                     VIGENVTIEKAMIGECATINDNVQIKNVNNEINVVSEYENIEPRCVLIEGGL"
     gene            complement(28915..29958)
                     /locus_tag="L3M60_001106"
     CDS             complement(28915..29958)
                     /locus_tag="L3M60_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBN6225795.1"
                     /translation="MGTKIVLSIVLIVVVVAISLTCIRVIKQSKVGIIMRLGKFQKVA
                     ETGVHLLIPFLDKMAYVIDLREIVIDFPPQPVITKDNVTMQIDTVVYYKVTDPVRYVF
                     EIANPIAAIENLTATTLRNIIGELDLDETLTSRDIINVKMRTILDEATDKWGIKVNRV
                     ELKNIMPPQDIQVAMEKQMRAERERREAILQAEGNKSAAILQAEGEKQSAILTAEAKK
                     EAMVRVAEGEKESAILVAEGEAEAIRQTAIAKAQGEAEMIKRTQIATAEGLKLVFSAM
                     KEADIDNNILALKSMEALEKMAEGKSTKLVLPSEAVNFLGTFKGIKEVMSDDNKEIFD
                     VKEALGDNESLKK"
     gene            complement(29979..30425)
                     /locus_tag="L3M60_001107"
     CDS             complement(29979..30425)
                     /locus_tag="L3M60_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBN6225796.1"
                     /translation="MKLIWLIVAVLFGIAEMLTPSLTLIWFSVGAVVLIFLSSFIESI
                     FLQILIFAVISIAMLVIATKKIVKKDKGYKSNTNLQAMMSKKGIVTEEISPNNTGLVV
                     VEHETWTAISIDGEKIEKGSTVEVLKIEGVKLVVKKVNATTSVTNQ"
     gene            complement(30595..32175)
                     /locus_tag="L3M60_001108"
     CDS             complement(30595..32175)
                     /locus_tag="L3M60_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBN6225797.1"
                     /translation="MLIGTSKLSRNVDTACVEEVGIPMIVLMENAVISAMKNMDIDMY
                     NSYTVVCGVGNNGGDGLGIARHLNTCGKEVNVFLVGNIEKLSECSKINYNVLLNMGIN
                     IININKNDLSEKNYGDENEVDKIIKVEKQNIIKNKNLTIFKDRVSKSDVVVDAIFGTG
                     LKRDITGIFKEVIDIVNDNSKNTYSIDIPSGIDSDNGNVLGTCIRANKTISFEFYKRG
                     FLNYETSALIGDVVVEKIGVPDFITRKYHDEEFITDKTFVKNNIRRREKHGFKSDFGK
                     VSIVAGSKGFYGASFIATESAVKSGSGLVTLISSEDVQEKVSIRLTEAMTLNFEEERL
                     SKLLSSSDAIGFGPGMGNNSQTFDKLLKIVENSNCPIVLDADGLNVMKDRCYKFLEWK
                     NRFVITPHLGEMARLTGDSIGYIREHRVDVAKEFAQKYNVVVLLKGYQTVITDGKKTY
                     INPTGNSCMATGGMGDCLLGMITSFIGQGMDILEATVSGAYIHGYIGDELSKTMYTVN
                     ATDLISNISLTMKELLDI"
BASE COUNT        10595 a         5877 c         3317 g        13173 t
ORIGIN      
        1 aaatataaaa ttttgcttat gtaattaaac tataacaaat taaagcataa tttaaggaga
       61 caaaattata atctgtctcc ttaaacacta aaactatgtt ttaaagaatt actaaatact
      121 atacaaattt caaatataat ataaaattaa ttacacttta acagtattag ctccatcttc
      181 ttcccatttt ttaatctctt ttagtctatc tattataggt aaatgctgag tacattcaac
      241 ctcacatttt ccacaagata tacattcttt agcttttgcc ttactattca ttgtataatt
      301 ccaatgtcct aagccataaa ctaactttgt catttcttca tcgctctttt taaacatctg
      361 tttttcattg taaaaaagca tataagctgg ggtattaata ccttttggac atactttaca
      421 atacccacag cctgtacata tttcgttcat attcttattt agtctagtct caatagcatg
      481 tatatcctca tctgaataaa ttctattttc gtctgcaatt ttacatgcat catctatatc
      541 ttgtttttta ttaaacccta tcaaagatat acttatttgt gaaattccta tgttgaatct
      601 taaagctgat tctatagtac tttcatcatc ttttgataga aaagaaagct ctttattata
      661 ctttggaata gttccaccac ctaatggatt cattgccaca acaccatatc cattatcaac
      721 agcatacttt gcacctttta atctatatgg aaagttcaat atattaattc ccatagttac
      781 accttcaaac ttattttcac ttagaatatc tataacctca tctcctggtt gatgagatga
      841 aaatactata tggtctatca atccttcttc tttacacttc aataatcctt catattgacc
      901 tccaggtctc attgctagtt cataatgttc catcttccta atattccaaa catgataaaa
      961 atttatttta gatacgccta atcgttctat tgattttttt acagcttcta tagctttttc
     1021 tgatgtatca catgcttttg gtttttcttt agttgataca taaaaatcgg tcttaccttc
     1081 ctttatcatt tgtctaaagg ctagtccaaa tatatcttca cttctgtcat cacaataccc
     1141 tggtgctgta tcaaagtaat taataccttt ttcatacgca tatttgacta aatcagcatt
     1201 ttcttgatta cttttttcta aatcaaacct taatcctcca taaccaacta ctgaaaccat
     1261 tttccccgtc tttccatatt caacgtatct cattgtaaat tcctccttag atatattaag
     1321 acttaatttc tttatagaaa atattatacc ataaataatt tttataaatt atttatggta
     1381 taatattttt tttataaatt atattttttt ataacttgca ttttatagct aaattaaaaa
     1441 ctataaaaag gttcaatatg gactattact tgagcattct cattaatttc ttcctgtatt
     1501 tgtttctcaa ttttatgtgt taaatcatga gatttctcta cacttataaa tgggtctacc
     1561 attacatgca tgtcaatata aatggaactt ttgctacctc tacttctgat attgtgaaca
     1621 tcccttacct cattaaattc aaatacaatt tcttttatat aatcctcatc tacagccact
     1681 ctatctacca aaattccaat agatggccta aatattccat aagctgaata aattatgaat
     1741 acagaaataa taataggaac tatagattct attattactg gaaatcctaa cttgacacca
     1801 aataaagtaa ccaatacgcc tagagataca aatatatcac ttcttgtatg caatgaatcc
     1861 gaaatcaaaa cataactatt tagtttagtc ccaactctat attcatacat acatacaaaa
     1921 atatttataa aaagtgtaat aattaaagca attaaacttt ctatagttat agcaggtaca
     1981 actggattag ctattcttga cactgcacta agaattatct taattgctat aacaaacaac
     2041 ataccactta taaataaact ggttataatt tcaaattttt tgtgtccata aggatgattt
     2101 ttatcttttg gtttggatgc aaagaaaata cctataagcc ctactatatt agatgctcca
     2161 tccgacaaag aatgaaatcc gtctgctgtc atactgtaac ttttaatttg attaccaata
     2221 atgattttta ataatgctac tgcaaaatta gcaaataaaa ttatccataa tacttgcttc
     2281 actttcttat agttatccat aacaaattcc ccctcccttc gattcaataa aaaagctatc
     2341 tatcaatatc aatcaaccag ataataaaat cgtatataag cacatttact atatttatat
     2401 tcaaaattgg ttgtacttgt tcattcaatt tgaaacttaa ttataaataa aaataaactt
     2461 cttttttatt attaaaataa taaaatatat tttacttcat acaaaaaaag agtgcctcat
     2521 gaactaaaaa gttcattttg agacactcca ttagagtttt gataaacaat taatttttta
     2581 tactaaacct tctttaacta agaatgaatg agcaacatct tctggtgatt taccaagttc
     2641 atccacttga tagttaagtt ctctcatagt ttcttcattt atttttcctg aaagcatatt
     2701 taaaactttt tctaattcag gatatttctt caaagtatct tctctcacta atggagctgc
     2761 ataatatggt acaaagaagt ttttatcgtc ctctaatatc ttaagattaa actttttaat
     2821 aagaccatca gttaaaaatg catctataac ctgagattca ttattatcta aagctgtata
     2881 tcttaaactt ccattcattg cttttacatc tttgaatttt aaaccataat ctctagaaag
     2941 tcctacaaga ccatcctctc tattttcaaa ctccaaagtt ggtgagaatg taaattcttt
     3001 accatattta gtcaaatctg aaatagtatt tattccatac ttttcagcag tttcaggcat
     3061 catggctaat gtgtaagtat tattaaatcc aagagggtca agtaaagtta actgattttc
     3121 tttttccata gtatctttta caacattata tgcttcatca gcatctttaa ttggagtatg
     3181 tctcattaca tttaccaaaa gtgtacctgt ataatcaaca tacatgtcta actcaccaga
     3241 tttaattgca ttaaatgcaa cagatgaacc acctagattt aaattcttat taactttaag
     3301 attcgtattt ttttctacta attcagcata aacattacca agtataagtt gttctgtata
     3361 atctttagaa ccaatagtta ttgtatcttt tttacttgaa aaactagaac taaaaacaac
     3421 gaacaacatt gaaacaacaa ctactacaga tattacttta agtgctatat aatttttctt
     3481 aggtgctttt ggatttaatc cttttggagt aactgcaacc tcaattttgc taaataaata
     3541 atcaactaac agtgcaatta tacatgatgg aattgcccct gctaaaatca tattattatt
     3601 tacagtttga actcctgaaa atactaaata tcctaaacca ccagcaccta taaatgctgc
     3661 aagagtcatc aaaccaacag ctgtaactgc agatattcta actccaccca ttattatagg
     3721 aagtgctaaa gggaattgta ttttaaataa tgtctgattt cttgtaagac caataccctt
     3781 agctgattcc aatactactg ggtcaatact tgttatacct atatacgtat tttttacaat
     3841 tggaagtaat gaataaacta ctaccataaa tatagctggt ttacttccta taccaagtat
     3901 aggtacaaga agacccaaaa gtgccataga tggaactgct tggactaagt ttacaaatcc
     3961 aagtacaagt tttctaagag atgagattct tgaaataatt attccaagag gaacaccaac
     4021 tattatagct attaaaatag atgtaacagt tagactcatg tgttgtatta aaagctctat
     4081 aatcttatct ttttgtaata gtataaagtt aaaaaaatta gccaagtaca ctcacctcca
     4141 tttctaggaa ttgttcactt aaaacagaaa gtaaactact tctagtaata agccctacca
     4201 gcttattttc atcagaaata actggaatat atccaaccga attacgattc attacattta
     4261 gtatttctac taaattatca ccttcattta catgcaaagg ctcacttgac ataatatcag
     4321 caagtaatat tgacttatca tcaacatctt tcatatcttt tacagtaaca atacctttta
     4381 atgtttttgc tctatctata attagtaagc tgtcaacctt actcgttctc ataatttcta
     4441 tcccttgagt tacagttcta gtggaattaa cagcaatagg attttttatc ataatatctc
     4501 ttgcttttat atattctgga ttatcccaaa ctctatcttc tccgataaaa tcttttacaa
     4561 agtcgtttgc aggttttcta agtatatttt cgggagtatc atattgtgct attcttccat
     4621 ccttcataat acaaatttta tcagctatct ttaaagcctc atccatatca tgagttacaa
     4681 atattatagt ttttttcaat tcatcttgaa gagaaaataa ttgttcttgt aaagatgttc
     4741 tcgttattgg gtctaaagca ctaaaaggct catccatcaa tattatttca gcatcagtgg
     4801 caatagctct tgcaacaccg attctttgtt gctgtccacc actaagctct gaaggatact
     4861 tatttaaaaa ttcaccacta tcaagtccta ccatctcaag tagttgcaat gttctttttt
     4921 caatttcttc aacggatttt tcctttttta ggcgtggaat tagctctata ttttctttta
     4981 ttgtaagatg agggaatagg cctgtgttct gtataacata tcctattttt cttctaagtt
     5041 ctatttcatt ttcctgagaa attggtttgc cattaatata tatctcacca gatgtaggtt
     5101 ttattaattt gtttataagc ttcaaggtag tagtcttccc acaaccactt gaaccaatca
     5161 aaactaccaa tgtacctgtt tctacaacta gagagatatc atctaatata acattatttc
     5221 ctattttttt tgttacattt cttatctcta tcataattta tcatcccctt tttcgaacaa
     5281 gcaaccttct tcaatattat aagttgtttt tataattata tcatactatt ttatttatgt
     5341 aaacaaagta cgacttttag agcaatattt gtctgaatat tgacacaaat ataaagtatt
     5401 tatatatagt gtgtaatata taatatcgaa aagcaaaaaa tataaaaata ttcataaaat
     5461 aatactagct tcaattatat aatattaaaa ctagtattat tttatttaaa caaaactcac
     5521 atttggatac tatccatgta ttcaaataag gtaatatctt tataagtttc ttttttatca
     5581 cttaaatttg atattgtcac tcccaatagc ctaatttgtt ttccattcat ctcaacattt
     5641 ctcaataaat tatctatacc tcttcttata tcattgtatt cgtcaatata atgttctaaa
     5701 ctaaggcttc tagttatctt tgtaaagtcc tcatatttaa tttttaatgt taatgttttt
     5761 ccaaacttct cagaactttt taatctatgg cacacctctt cacaaagttc atcaagaatg
     5821 ttgcgtgttt cttcttcatc aatattcaaa ttatgactta atgtagtctc tgccccaaca
     5881 gattttctaa ccctatttgg ttctactggt ctatcgtcta ttcctctagc aaatttatat
     5941 aattcataac ctcttttttt aaatatattt tcaagctcaa ataaatttaa acaccttaaa
     6001 tcatagccag tttttatacc taaattttta agagtatttg aagtaacttt tccaacacca
     6061 aaaaatttat tcacaggtaa tttgtctaaa aagtcctggg cattttcttc ggttattaca
     6121 gttaatccat ttggcttttt taagtctgat gcaatcttag caagaaactt attgtatgat
     6181 acaccagctg aagaagtgag accaacttct ctaaatatat ctttttttat ttgctttgct
     6241 atctcaattg atgaatcaat atttttttta ttttttgtta catctaagta agcttcatct
     6301 aaagataatg gttcaactaa atctgtgtat ctataaaata tatctcttat ttgctctgac
     6361 acactcttat aaacatcaaa tctaggtctt acaaaaactg catatggaca cctattgtaa
     6421 gctacagctg atggcatagc tgaatggata ccaaactttc tagcttcata agaacatgtt
     6481 gcaaccacac ctctgctttg tggatttcca ccgacaataa caggtcttcc ttttaatttt
     6541 ttattatctc tttgttctat agatgcataa aaagcatcca tatctatatg aattattttt
     6601 ctatgaagtt ccatcatatc acctttaaag tataaataga ttaattttat ttatattact
     6661 ttttaattat accaaactta tgtttctttt tggtattttt tcattatttt atttatatat
     6721 tcatattttc aataaataaa accacctata atccattaac ttcaatataa tttataaaaa
     6781 atccttaaaa acacttgcaa aaactatata aaagctttac aataggtatt ccaaggaact
     6841 aaatttaaaa ttccttatgg ataacagtat atatatttaa tttaaatggg atgttttaaa
     6901 aacaaaagac tgtcttatac aagacagcct actgatttta ttataattgt aatggactgc
     6961 tcaaactatt actttaaaac agcccatatt tttttatata attttaactt taactatagt
     7021 aaagattttg tgaaggatac tctgggtcac aagttacatc tataccaagc tttctaaatg
     7081 tttgttcatc atccctattt aatatagtag tagaatgagc ttgacaccct tttaataagg
     7141 ctaacttttc catagcaaat tgtgcagttg gattagttac agcacaaata ctaagtgcag
     7201 ttaacacctc ttgacaacta agtgaaacat ttctactagc taaagttttt gtttttaagt
     7261 ttatgatagg ctctaatatt acaggtgata ttaagtgcat atcatcagat atattagcta
     7321 aatgttttat tgcatttaat actgctgctg ctgttccatt cattaaatca gaacccttac
     7381 cagttaatat tgtaccatct tctaattcta atgcaactac aggacaagtg tcattcttgt
     7441 cagcactttc tttcaattta ttactgtaat ttctagcagg taatacaaca tttctatctt
     7501 ctggctttaa gttaagttct tccataatca ttttcattct gtcataagca cctttatcaa
     7561 cttgtccttt tttatactca caagcagttt taaagtatct tcttattatt tcttctatag
     7621 aagcttttct tattacttca tcatctatta ttccaaagcc aactctatta acacccatat
     7681 cagttggaga ttggaaaatt gattcttttc cagttatctt ttctattatt tttttaagta
     7741 ctggaaataa ttctaaatct ctattgtagt ttactgacat ttcaccatac gtttcaagat
     7801 ggaatgaatc tatcatattc acatctttta aatcaactgt agctgcttca taagctatat
     7861 tcaaaggatg ttttacaggc acattccaaa ctgggaatgt ctcaaactta gaatatccaa
     7921 ctacattacc tcttttatgt tcatgataca actgacttag acaagtacca agttttccac
     7981 tattaggtcc tggtgctgta actactacta ttggttttgt agtttctata tattcatttg
     8041 ctccatatcc ctcatcacta actatagtct caacatctga cggatatccc tttgttggat
     8101 agtgcttata aactttaatt cctctacgtt ctaatttatt tataaataca gttgtagctg
     8161 gttgtccttc aaatctagta attactacac tatttacatc tagttcataa cctcttaaat
     8221 catctattaa tcttaaaact tccatatcat aagtgatacc aaagtctcct cttattttgt
     8281 ttctttctat atctcctgca tagacacaaa ttacaacttc aactttttct ttcatatgtt
     8341 gtaatacttt tatctttgca ttttcatcaa atcctggaag cactcttttt gcatgaaggt
     8401 ctcccatcaa ttttcctcca aactctaaat aaagcttgtc aaaattattt actctttcta
     8461 agatatattt agactgctct tctaaatatt ttttgtggtc aaatcctact ttcatcttaa
     8521 cacctcacta aattaaattt cttgttgata acagtatatt tatatatatt tttttagtaa
     8581 aacatcttat aaaaaaatgt taatactttt tcactcaaag taatattata caattaagat
     8641 taattttttt caagatagtt ttatggtaaa taagatatac ttatataact aataaaataa
     8701 gaatattata tttttatata taagtataat attctcacct tagtattaat ttatttcaat
     8761 attttcatat atcttttact ctaaattcaa cactttccta atatcttcta tcccttgata
     8821 aacttcttgt ttagaaaaat tcatagcttc taattcttca tcagttttct ccataagcat
     8881 agtataaaat tctattgcta tatattgcat attttcacta tttaccgtct tagctttatc
     8941 aaataaaatt tcttctgctt cgtttacttt tccctgaaaa actaatcttt ttaaagttat
     9001 aagtataact tcttcttctg tattatacac taaatctttt tcttcatatt cactgtattt
     9061 tttccccaat aaaagtcttg caaccataag ttttatctca ttgaaaatct tatccactat
     9121 agccatgttt acatctccta taaataaaat ttattttctt tttaattata tcagacttta
     9181 tagtaaaaat tattatatgt aaatgaaata atacaaaaat aaattttaac aataaaaaaa
     9241 gaaggtagcg ggggatttcc gactaccttc aaacactaga ttgtgattgg tttggtattt
     9301 tgtttatacc caccaaaagc tttgttaaat catctaaatt gattttttta gcatccacat
     9361 cctccatcaa aaacgtcacc acattcacaa agtattaaca ataatattaa gaaaggtatc
     9421 catgcacata agtccatttc agcccaacaa tcttggaatg ctaagaataa gaagaataat
     9481 attattatcc accaagcaca tccaccaaat ccgccttcac caaaaaatct atccaacata
     9541 atatgttacc tcctagccat tttgtatgtg gagtttcagc tcctaaaata tactatgttt
     9601 aaggggtaat atttgctaat gattttttaa aattttttta gatattttaa aaaaaattat
     9661 tgattcacat tcaacgtctt accatcactg ttgataacta cctctccatt agaagtcaaa
     9721 aatgttttta tattcaattt ttccagtatt tttaaaatct cttcatctgg ttcttttttc
     9781 tccgaacagg taataacagc atatttaggt gaaatagatt ctaaaaatgc ttcagttaat
     9841 ttgtcatatc ttccatggtg gggtatttta acaaaatcat attttaaagt atttccattt
     9901 ataaactctg acaacctttt ttcctctgca tctcctgcaa ataaaaactt atgtgctcca
     9961 tagcttatac tagtaattat tgaataatca tttgaacttt cgtacttgct ttttaaagct
    10021 gattgtatgt ttatttctgc actattaatt acaatattca tattctcttt tagtagtact
    10081 ggttctatat cagccttttt aagcgagtca atatattcat catattgctt actatctttc
    10141 ttataatttg cctgtataat attctttact ttaacactag ataacacact atctactcca
    10201 cctatatggt ctttatctaa atgagttaaa atcaaatagt caagtgtatt tattcctttt
    10261 tcttttaatc tatcaactat ttgttttcca tttttatctt ctccactatc aatcattatg
    10321 actttgtcac ctatctcaac taatatacaa tcagactttc cagtgttcag tatagtagtc
    10381 ttaaattcag tattttttgt gctactctga atatcatttt gactcacact atttttgtca
    10441 ctttttaaat aagattttat tgttgatttt atcttaccat tagcagttat atttactaca
    10501 atcaataata ttactattag agataatgta gtaactaaaa acaggatgtc ttttaatatt
    10561 atttttttca tccaaaagct cctataaatt ttatttatta catatttata gtatagcaat
    10621 cttaatgttt taaaatacac atataaaagg ggcaaatttt aggcatattt gtggcatgat
    10681 aaaaaaatga ctgtatcaaa atgattatct ttctaaattt acaccttcaa attctttaat
    10741 cttatcatta gataaaacac tctttttagt agattgtcca ttttttctat caaaagtatt
    10801 tttagctttt acacctgata aaaacttacc tgctccatta atacaacctt cttcacacat
    10861 cattccttct ataaaattac cttctaatct tcccaattta gccattgtca ttgtcttttt
    10921 aatttgtctt ggaccagata cttttatagg ttcaaaatca aaactaagcc ccttttcagc
    10981 aatatagttc tgtattgatt tagcaagtcc accagatgct ccaaatcccc ttccaaaaat
    11041 cgaaccatca tttatatctt catttgaaca ttttgataaa tctatattaa atgcttcaaa
    11101 taatgccatt agttcttcaa aagtaagtac ataatctact gaatctttta aacatttctc
    11161 tgttgcctca ctctttttag ctgtacatgg tcctataaat acaaccttgg catcagaatc
    11221 tatttttttt actaatcttc ctgttgctac cataggagac actgtagatg atattctatc
    11281 tttttcatct gggaatatct tctctatata cttaaaaaat cctggacaac atgaatttgt
    11341 catgtaatta tctcctgatt ttattctttc aataagctca ttactttcat gaactgtaac
    11401 tgcatctgct ccacaagaag cttctatcat atctataaat cccaatgatt ttatagcatt
    11461 ttttaactga ccatatgtaa tattactatc tatttgacct gttatagctg gagctactat
    11521 tgcatacata ttttcctttt ttgcaagttt ttttgcaaca ggtgctatta aactcttatc
    11581 agatattgct ccaaatggac atgctgacat acatgctcca caatttatac aattatcttc
    11641 atgtatcatg gctttcatag tgtttctatt aaaatccaaa gctcctgttg ggcaaacact
    11701 cttacaaggt cttacaactt ctgatatagc atcatattgg catgaacttt tgcacattcc
    11761 acaagcctta cataaatcat gatttatata tgctctccca tttatatagg aaattgcccc
    11821 aaatttacat gcctccttac atctatgagc caaacatcct ctacaaagtt ctgtaactgt
    11881 aaattcattt attggacatc tatcacatgc tgcttctact atatatataa tttgctcgtc
    11941 tgtgtccaaa ccaactgaat ctggatttat atctccatat ttactatttg gagttaagct
    12001 taatgcaagt tgcgcccttt caagaactac ctctctctct ttataaacac agcatctata
    12061 tctcgccttt tctcctttaa ttatctcata tggaattttt tgtatatttt cttttgttaa
    12121 attttcttca atagatagtt ttgctactgc tgtcaaaact tcatgtttca atctttttag
    12181 ttgcgtttca aactcaaaca taagtaatcc tccctataaa tcacatcttt ttgctatatt
    12241 tctactgtta tgtatattgt atattttatt tatttccata aattattata acataggcat
    12301 atactttgtt aagttatata caaaatatta tatatgtaat ttttatttta agcgtatata
    12361 atttttattt tagttgttca ataattaaat atataaataa aaatagagtt gtcctaaagt
    12421 taaacttcaa cttagaacaa ctctattaac aatatgtatt tttgttgctt tgtattatct
    12481 ctatttttct aaatttatat tactaaaatc ttttatcttt gtatttgcta agacactctt
    12541 cttagttgat tgagaatttt ttctatcaaa agtggcttta acctttgcac ctgataagaa
    12601 tttaccagaa ccatttatac aaccaccttc acacatcatt ccttctataa aattaccttc
    12661 tagctttcct aaattagcca tagtcatagt tttcttgatt tctcttgaac cagaaatctt
    12721 tacaggttca aaattaacac caataccttt ttcagcaaga taattttcta tagcctttgt
    12781 aagaccacca gcagctccaa aacctcttcc aaaaattgat gcatcatcta tatcttcatt
    12841 tgaacactta gccaagtcta tatcaaatgc ctcaaataat gccattattt cttcaaaagt
    12901 aagtacataa tcaacagctc cttttaattc tttcttagct gcctcactct ttttggctgt
    12961 acatggtcct atgaatacaa ctttagcatc tggctcatga ctttttataa atctaccact
    13021 agctatcata ggagatactg tagaagatat ttttcctaca tgatttgggt atgttttttc
    13081 tatatagcca acaaatcctg gacaacaaga atttgtcata aaatcttctc cattttccat
    13141 tctttcagca aattctaagc tttcgtgaac tgtaactgca tctgctccac aagctgcttc
    13201 taatacatca acaaatccta gtgattttat agcatttttt atttgaccat atgtaacatt
    13261 tgcttcaact tgacctgtta tagctggagc tactactgca tacattttct ctttttgaac
    13321 taattttcta gctacaggag ctatcaaact cttatcagat attgctccaa atggacatgc
    13381 tgacatacat gctccacagt ttaaacattt ctcttcatgt atcatagcct tcatattttc
    13441 tctatcaaac cctaaggcgt tagttgggca aacactctta catggtctaa caacctctga
    13501 gatggcatca tattgacaag cttttttaca cattccacaa gccttgcata attcatgatt
    13561 tatataggcc attcctccga catgtgttat agctccaaat ttacaagctt ctttacatct
    13621 atgtgctaga catcctctac aaatatctgt tactgtaaat tcatttattg gacaactgtc
    13681 acatgcagct tccaaaatat atataatttg ttcattttct tctatatcca ttgtatctaa
    13741 atctatctta tcttgacctg gttgtaatcc catacttaca tttactctct cagatattat
    13801 agctctttct ttataaacac agcatctgta tgtaggtttc tcacctttta ttatttcata
    13861 ctgaatatta tccattgctt ctctggtaag gttatcttct tttccgagtc tagctattgc
    13921 agttaaaaca tcatgtctta atttgtgtat ttgtgtttca aattgaaaca ttttaatacc
    13981 acccccgtaa atgattttat acgataccat tataacacaa tatttccttt tattaatata
    14041 tacataaaat attatatgtt taaattttat tttatcgaat atatatagta ttttttcatc
    14101 ttattatata atattattga tatttctgaa atttcactat ttttattttt acttttcaat
    14161 cttatatata atattttttg ttatatattt taattttact ttttacttac ataccttttt
    14221 attacatttt ttgatattaa atttattatt tgtatttaca tttccgtcaa attttgtaat
    14281 acttctcact atataattta acaattttta ttaaattcta aaagtaatta atttttttaa
    14341 aaattttatt tgcttttatt taaattttat gttatattaa tagaacagac ttattttttt
    14401 agtttgtatt ttttaataat gcggaggttt tcttataatg aaaaacggaa tagtaaaatg
    14461 gtttaacaat gaaaaaggat ttgggttcat atctgtagag ggagaagatg atgttttcgt
    14521 acatttctca gctatacaaa atgatggata caaaacttta gaagaaggcg aaaaggttag
    14581 ctttgatata actcaaggta atagaggacc tcaagctgaa aacgtaaata gaatataatt
    14641 atttaattta aatatatata ttttagaaaa ggagctatcg agggctcttt tttgttgttt
    14701 attatattta tttttaatta caaaaataag aagttacttt agaaaaacgt taaacttaaa
    14761 ctaatttttt taaaataact tcttatatta taatacatat tattcatata aaatactcta
    14821 tttaattttc tcagatatta tcaagcataa ctctcttaag attttacagg ctataactgt
    14881 cgatacacct gtagtatcat aatcaggact taactctaca atatcacaac caactatatt
    14941 tatattagaa ttttttataa tcttaaaaat ctcttgaaac tctctatagt ttacaccacc
    15001 aggttctggt gtacctgttc ctggaaatac agatgcatcc aatacatcta aatctatagt
    15061 aatataaata ttctttccat ttagcatatt aactatcttt tcaaatgtat caattccgcc
    15121 tatttccatg tatgtgtgtt tttcttctgt agcaaattta aattcttctt ttgtcccaga
    15181 ccttatacca aattgaaata ttttattatc acctacaata tcccatattc ttttaattac
    15241 tgttgcatga gaatttttgc tattattata ttcttctctc aaatccgtat gggcatcaaa
    15301 atgaattaca tatatatcat tgtacttttc atatactgct ttaaaggctg gtaatgtgac
    15361 taaatgctct cctccaatca taaatggcac ttttgaatct ctaacaatct tatatgtttc
    15421 ttgatagatt tcctttaaga cttgttctgt acttccaaca ctaatttcta agtctccata
    15481 atcacatata ttataatcct ctaagtccaa atctaaaaaa ggactatacg tctcaagacc
    15541 gtaaaattct tttctcatag agctacttgc aaatctagct ccaggtctat tagaagtagt
    15601 tccatcaaat ccaacaccaa atactataag attagattct tcataatttt tgtccatact
    15661 cataaaggta ttcatatgat agaaattatt cttcatctag catctccttt acatattgag
    15721 gtaacataaa cgaacctaaa tgtatatcac tattataata tttagttttt aaacttaatt
    15781 tctcccaccc atttctatct tgatttttta ctggattaag tttttttgaa gcaaatccaa
    15841 acaaccagtg accagatggg tatgttggta tatgtgcttg atagacttct gatattggaa
    15901 atatttgttt taacttttta ttagctcttt tcatctcttt agcattaaag tcaaaatacg
    15961 gactttcact ttgatttact aaaattccat catcagtcag tattctataa caatccttat
    16021 aaaaatcagt tgagaaaagt ccttcgcctg gtcctattgg gtcagttgaa tctactatta
    16081 ttaagtcata tgatttatca tgtgcacatt taacaaaatt aacgccatct tcaaaatata
    16141 aattcactct ttcatcatct aatttacatg agcatatatc catatacttc ttagatacat
    16201 ctacaaccat tttatctatt tcaaccatat caatcttttc tatttgagga taacggctta
    16261 attctcttac tgtacctcca tcacctccac ctatgactaa tactttcttt atgtttaaat
    16321 ttgtagccat aggaacatga gttatcatct catgatatat aaattcatct ttatatgtta
    16381 ccatcattaa cccatctatt gtcagaaatt ttccaaattc atcactatca aatacgtcta
    16441 tcctctgaaa ttgagatttt ccttcaaata gatgtttatt tacttttata gaaaatctta
    16501 cattttctgt ccattcttct gtataccaaa gttccatata ataatcctca ctttctcttg
    16561 tatgttttat actgcttctg gtttatgagt aatcttacct aaatcttttt ttgaatattt
    16621 tttgattttt gtagttaaac ctcttggaat ttctgtagac tcgcttcttt cagattttaa
    16681 aagttgctct aataattcaa aacttttcca tgggtctata tcaccacaag taaaaaaatc
    16741 tgctgctgca taaccatatt caggccatgt atgtattgtc aaatgagatt cagcaattat
    16801 aacagctcct gaaacccccc aaggattaaa atggtggaat acagaatcaa caattgtcgc
    16861 ctttgcattt agtgctgaat cattcataaa attttctata agacgtgggt ctttaagtac
    16921 gttttcatca caattatagt attctactag tatatgtctt cctagtgttt caaatttcat
    16981 ttcaacccca actcctttat acttttaata agttctatct gataaaatct atttaacaaa
    17041 cacttgccct tgcaaaataa aataatttat tttattacta gtattctatc taagttttta
    17101 tcttgtacat ctgtaagata tgcattgcta ttttttaaga aaataatata ctccataatt
    17161 tcttctgtta tcaattctcc tggagcaata atcggtatac ctggtggata tgccattatt
    17221 gattctccac aaattctatc tatacatgaa ttaatttcta cactttcctt tggtgcataa
    17281 aaagcttctc ttggattaag tttaataatt ggatttattt gaaccatatt aaattcattt
    17341 aatgttgatt cttttctata aatctttgca attatagaaa gtgcttttac taatctatct
    17401 atatctgatt tatttgttcc aatagacaca agtgccagaa tattatatga atctcctaat
    17461 tctacttgaa ttgaaaaatt tttatataat aaatcgtata cttcaaatcc agtcaaattt
    17521 aattctttta catttataca caattttgtt tcatctataa atttaacacc tttttgtttt
    17581 aatatttctg tagataaaac ttttattcca tcaattttgt tcaatttaga ttttgcatac
    17641 ctagataggt ttaatgcttt tgacagttgc ttttcaccat tctcaactag attaactcta
    17701 gcaccatcta tacttgccat aagcaaataa gatgctgatg ttgattgtag catattgatt
    17761 gattgaagaa ccttttcaaa gcttacatta tctctattca ataagagtgc tgaagattgt
    17821 gtcaatgctc ctccagtttt atgtatactt acagctacca tgtcagctcc caaacttatt
    17881 gcagaaggag gtaaatctaa atgaaatgga aaatgtgcac catgagcttc atcaactaac
    17941 actaaaacat tatttttatg gcatagttct ataatactct caaggtctgc acaaactcca
    18001 tagtaagttg gattaaggaa aaataatgct tttatattac aatctttttc tatctctttt
    18061 ctaacttttt caaaagtaat atttaaactt atacctaatt tttcatcaaa ttctggttgt
    18121 ataaatattg ggattcctcc acaaagtatt aatgcattta tcactgattt atgtatattt
    18181 cttggaagta atactttttc acctggtttt ataacactca aaatcattgc atgtattgct
    18241 tgagttgtgc cattcgttat aaagaatgcc tcatcagcca tatatgcttg tgctagcaaa
    18301 tcctgtgcat tttttataat cccatttggg gcagatacat tatccatagt tggtgatgaa
    18361 tttatatcca ttttcatgag actttcacct agatatttat ttaatatagc tacacctcta
    18421 ttttttacat gtccaggtac atcaaaacaa gctatgtctt tctctgagta ttcctttaac
    18481 gactctaata atggagcaca ctcatagctt aattcaactg catttgcaaa ctcgctatcc
    18541 atgattttta aattcatctc ctctctatat tcaaacattt ttaatattta ttatttatta
    18601 tttctatttt ttatttttac ttttattaaa ctcatgatat ttattatatt aacattccag
    18661 catttttaca agtgtttttt tgaagttaat caaaaacaca tatatcagtt atatcaatat
    18721 ttataaactt ttttattttt tgttgtttat attaattttt ttaaaactta gtatttataa
    18781 acttttttat tttttattgt tttagcattt ttattttttt gagttttagt aagcttttga
    18841 gtttactatt tttaatttat aagtatttta ttactaaact tttatattat tctcatttta
    18901 tatataaaaa gtcttggttt agttgaacta aataattttc ctatttttgt attttaattt
    18961 tatattaaaa tttattaaat tttttaattg ataaactaaa ttctatattt ataatattaa
    19021 aattctataa gctccttgat ttacatatgt tgattttaat atactacata ttgtagtgtc
    19081 ttagagcttt tatttgtatt ttaaattaaa tttattcttg tttttccttt aataattaat
    19141 agtaaaacaa aaaaaatata aaaaaaataa agagaaactt tataaaagct tctcttattt
    19201 catctctgct ataggtatcg ttttccaatg ttctctcaaa actctttcaa tattttctaa
    19261 gtcattctct ctcataaatt ttactacttc aatatgttga ttattgctgt atgtcaacat
    19321 tttatataac tcatcttcat tatcactagt atatgacttt gacataaaat tgtatcttaa
    19381 agagttcaat agatttacta acacctgatt atttgattta gttatgtata tatcgtgaaa
    19441 ttcattgctt aattttaaat agctactgta atttttatac tttatagaga ccgccatcat
    19501 ttctattaat tcttccattt tcactaggtc tgattcagtt aaattttgta atgctgaaga
    19561 accggctaac gcatccaata ctccaattac agaataggtg tctaatttct ccttttcttg
    19621 aaattctttt actacaaatc ccttattagg tattttttct agtaaatttt cacctgctaa
    19681 cctagttaat gcttctctcg ctggtgttct actaactccg atagatttac aaatctcaga
    19741 ttcagtaatt ctctgattag cttcatactc tccattttgg attttctttg atatatagtc
    19801 atatacttga gttgtaagtg gatctacctt cataagacgt tcctccatat taagattata
    19861 aaagttaaat ctccctacaa ctttggtaga gagttcagtt ttatcttact aatttatata
    19921 atatcacaat tatgatattt tttatacttc tttgtaatat tttgaaatac taaaattctt
    19981 atgataattt atacaaaaaa atgtagaaaa agttttgata aaacaaaact ctttccacat
    20041 tccccttatt ttctatttat ttttctaaac aaatatattt aatttactct aaaaattcta
    20101 aatgatttag actctaaatc cactttcaac tcattatttt caatttgtat agtactattt
    20161 acatttttaa gactatactt tataggtatt tcctcaataa aactactatc catacataat
    20221 gatatacttt ccacattttc acttctattt actaaaatta atattctatc attattttca
    20281 ttaactctaa taaaggaaaa tacagaatca ttattagtat atataaattc tgtatttcca
    20341 gaacttaaaa gtttattctt atttctaatt tttataacat ctttataaaa attaagcata
    20401 tcttcatctt catttttcca tggatatgtt cttctattat ctgggtcaac acctccacaa
    20461 agacctgctt catctccata gtatatatat ggtactcctt caaatgtcat ctgtatagct
    20521 acagcaagct tcactaaatc tacatcatca tttaattctg ttttagctct tgtaacatca
    20581 tgtgttccaa ttaaatttaa attagactta aatgattcct ttggataatt ttctttaatc
    20641 tgcatatatc tattacaaag ttcgtatgaa ttaatttctc cttttaagaa agaaaacatg
    20701 ttatttctaa aaggataacc cataacagaa tctaattctt cacctaataa ataacttctt
    20761 ctctgaccat agctaatctt atttgatgca tcttcccata cttcacctat aagaattgag
    20821 tcatcatccg ccttttttaa ttcctttttt agttctctta taaacttagt tggtagttcg
    20881 tctgcaacat ctagtctcca gcccttaata cccatattta tccatttatt tataacgcta
    20941 tccttatcat atattatata atccatgtag ctatgttcta attcatttat atttggcaat
    21001 gtcttaacat cccaccaact tttatacttt tggggaaatt cttcaaacat ataccatgaa
    21061 taatatggag attcttttga ttgatatgct cctaaactat tatagtttcc atacatatta
    21121 aaatatttac tatctgctcc agtatgacta aaaactccat ctaatactat tgatatacct
    21181 ttttcttttg ctttattaat aagttcttta aatgtatctt catccccaaa cattgggtca
    21241 atttttttat aatcacctgt gtcatactta tgattactag aagcttcaaa tatagggctt
    21301 agatatagta tacttacacc taattttttt aaatatccaa gtttattgat tatccctctt
    21361 aaatttccac catggaaatc ccaccttata acatcccctt gactatcctt tatgtacata
    21421 ggtatatctt cccaatttcc atatatgaaa ctattttttt taggattatc aaccttacca
    21481 ctacgattcc cattattaaa tctatctaca aatatatgat aaagtattcc ttctttgtac
    21541 cacgttggta ctttaaaatc tttgtaaaca gtgatttgat atttattaat atcactataa
    21601 ttatattcac aagagtatcc gttatctctg ttttttccat aaaataaagt ctttatctga
    21661 ccatccattt caacatcaac tttaaagtaa taaaaataaa cacctgtccc atcaaacttt
    21721 tcaatcttac cacaaaagca ctttccaagt ttatcattat ctaattcttc atttaaagtt
    21781 attgttctca tttcttcttc tctatttatt tctaggaaaa tagacttgac attgtatccc
    21841 tcatttactt taacacaaat gttcaattcc tcatcaaatt ttaaagctcc aaacggagcc
    21901 ttaaaatttt tatcccaaga attatattct attacacttt gcatttaatc acaccctttt
    21961 aaaccaaatg tcttttgcat actcagaaat tgttctatca gaagaaaaaa atccagagtt
    22021 agctatgtta actaaagaca ttttattcca tttttcttta tctctatata acctgtttat
    22081 atctgcttga gcttgaccat aattttcaaa atctctcaat acaaaatact catcattgta
    22141 agtagtgagt gagttataaa tacttcttcc ttcttcgcca agattaggaa taaatccatt
    22201 tattaaatca tctactaccc tttttatatc catattagaa tgatataggt caagtgaaga
    22261 atatccacca tatttattat aatttagtac ttgttctgca ctaagtccaa ataaaaacat
    22321 attttcttta ccaacctgtt cacatatctc tacatttgca ccatcaagtg tagccaaagt
    22381 tatagcacca ttcatcataa acttcatatt acttgttcca gatgcttcct ttgttgtagt
    22441 tgaaatttgc tcactaacat ttgctgctgg tattattatt tctgctaatg atactccata
    22501 attctctaaa aatactactt ttaacttgtc ctttactcta acatcattat ttatagtact
    22561 tgcaacagaa tttataaatt taattataca tttagccaaa taatatcctg gtgctgcttt
    22621 tgcaccaaat ataaaagttc ttgggtcaag atttaaatta ggattatcaa gcaattcatg
    22681 atacatatga agtacattaa atatattcag taactgtctt ttatatgcat gcaatctctt
    22741 aacttgaaca tcaaatattg aacttgggtc tatatttaaa tcgtatttat cttttataaa
    22801 ctttgctaga tttgctttat tattgtattt tatatcatca agtctttgta agacagaaga
    22861 atcattttta aatttttcaa tattttttaa ttgtaaggta tccctcttcc atgaatcacc
    22921 aattaaatct gtaattaaat tgctaagttg aggatttgaa cttataagcc atcgtctatg
    22981 tgcaattcca ttagtcttat tattaaattt atttggttca tcttggtaga aatcttttaa
    23041 tacctctgtt tcaagaatat gagtatgaag cttagcaact ccatttacac tatgacttgt
    23101 cacaatacat aaatttgcca tattgatatt gtcatagtct attatactca ttctttttac
    23161 tctatcttgg tcatatcctt tattatttaa ttcctctaca tatcttctat ttatttcttc
    23221 tattatcata taaattcttg gtaatagttc tttcatcata cttacaggcc atttttccat
    23281 tgcttctgac attatagtat gattagtata agacactgtt tttttagtta tttcccatgc
    23341 ttcatcccaa gataaatttt cttcatcaag caaaattctc ataagttctg gtatgcataa
    23401 ggtaggatgt gtatcattta tatgaattgc taccttatcg aataaattat tgatatttaa
    23461 tttgttcttt ttatattttc ttattatatc ttgtatccct gcacttacaa aaaagtattc
    23521 ttgtttcaac ctcaacaact tacctgcata atttgaatca tcaggatata acacctgtga
    23581 tatttcctca gtatagtact tatgttttaa agcctcttca tagctaccag agtaatttag
    23641 agcatttgag gtaagctcac caaaatctct ctttggtatt tcactcttaa acaatcttaa
    23701 agtatttata cattggtttt ggtatccgat tattggtata tcataaggca ttgccattac
    23761 aggaaggtag tttttatgaa ttacttttaa atgactacct tccttgatta attctacctc
    23821 accaccaaac ttcaccattg ttgcttcatt tggtcttaca gtttcccatg catatcgtcc
    23881 ttctgtaagc cagttatcag gcacttctac ctgataacca tttataaatt tttgttcaaa
    23941 cagaccgtat ttatatctta ttccatatcc ttgaccactt atgttcaaag acgccataga
    24001 atctaaaaag caagctgcta atctaccaag cccaccattt cctaaggctg ggtctacttc
    24061 agcttctatt aaatcatcca agttgattcc taactctgac aaaccttctc taacttcttc
    24121 ttcaatatct aaatttaata aattagattt taattgtctc cctattaaaa attctaaact
    24181 aaaataataa acctcttttt tcttgtctaa ctttgtagca acccattttt ttgctatttc
    24241 atctttcaat agactacata atacatttaa caattgttca tcagtagctt ccttgataga
    24301 ttgtgcatat aagctataca tttttacttc aaatttttgc ttgaatttct ttttgcttat
    24361 tgttaccaaa aaaaccactc ctttaatcac atatgtctct atatgtttct atatactcct
    24421 tagctgattt tttccaacta ttatctgttt tcattgcatt ttcaactaat ttaatccatt
    24481 tttctttgtc cttaaacact ttgattgcat ttttcaaaca gaaaaacatc tcatgagcat
    24541 tgtaattttt aaaactaaat ccattgcctt ctccagtaaa tttattataa ggtgttactg
    24601 tatccctaag ccctccagtc tccctaacta ttggtaaaga accatatctc atagctaaca
    24661 tttgacctat accacaaggc tcaaataatg atggcattaa gaacatatca cttgcagcat
    24721 atatctgttg agccaatgat gcattgaaag taattctagc tgatacttta tctgaaaaat
    24781 tagaatcgta atagttgaac attgattgat attgatgttc tccagttcct aaaacgacaa
    24841 tctgtaaatc ttctctcata agttctggca tcatgtatgc tattaaatca aatccttttt
    24901 gagaaactaa tctcgaaact atacctatca acggaataga tgcatctttt ttaagtccca
    24961 atatatcttg taatctcaat ttattttcta cttttttgtc aattgagtct acatcataat
    25021 gaacaaatat atctttatct tttgcagggt catttaagtc ataatctata ccatttaata
    25081 taccttttaa ttttcctgac tcttttctaa gtaatccatc taattgttca ccataaaaag
    25141 atgtttgtat ttcatttgca taagtaggac taacagttat aattttatct gcaaaattta
    25201 taccagcttt cataaaattt attgagccat aatactctat atctccatta tcaaagtatg
    25261 tttgtggtaa gtccaatata tcatataaaa catgcttatc aaatacacct tgatattgga
    25321 gattatgtat agtatacata gtttttatat ttttataccc ctcaagggtt gcatatcttt
    25381 cttttagaat taatggtaac atccctgtat gccaatcatt tatatgtatt acatctggat
    25441 aaaaatcaat tctacttata gattctaaaa ctgcatttgt aaagaaagta tatctttctg
    25501 catcatctcc atatccatag atgctctttc tttcatcttc tcttctaaaa taaaactcat
    25561 tatctataaa gtaaaatttt acaccatcaa gttccatttc caaaagacca cagtattgat
    25621 ttctccatcc aacacgtaca ctaaacacag ctatttcttt tagttgatct tttaaataac
    25681 ttggtatagt tgaatatttg ggcattatta cccttacatc tacaccttct ttttttaatt
    25741 ctttaggcaa tgcataagaa acatctccta atcctccagt ttttgcaaag ggccaacact
    25801 ctgctgttac ataaaaaact ttcattagtc attccccctt ctaaaaaatt tatttatata
    25861 tttgtattct tttccactac aagaggatat ttttcatctc ctcttaattc tttcccattt
    25921 gatacactcg tatttttatc taatattgca ttatataatt ttgcatttga ctttattaca
    25981 caattttgca ttactattga gttctctata actgttcctt tttcaacatt tacctttcta
    26041 aatatgatac tatcttttac agtcccttca attaaacaac cagatgctat aaagctgttt
    26101 tctactttag cactatctgt ataaattgta ggtgattcat tcttttcttt tgtaaatatc
    26161 tttctttcag aatataataa ttcatcagca acttctaaat ttaataaatc tttgctcatt
    26221 tcaaaatatg attcaacaga atttacacat tttagatatc ctgtatattc ataagctcct
    26281 attttcatgt tttctgcttc ttcaataata aattcttcta aataacaata attgccttta
    26341 tttacaatgt tatatataca ttcaacaaaa tcttcacgtt tcataatata cgtttccata
    26401 catatatttg ctctagggaa tttacccaaa ttatttccta catttatcac tctattgtta
    26461 ctatctaaat ttagagttaa gttaccagca aaatctttat ttgcattatt tacattttta
    26521 tatattatag ttatatcatt atgagatttt ttgtggtatt ccaatgcttg agaataattt
    26581 aaattacata gcatatagct tggagctatt aaaatatatt cttcttcact cttttctata
    26641 taatctagat tagctaagat agtatatata tcaccttctc tatgtgagta agtatttcta
    26701 tacttagtgt tttctggact aaaaattgat aggccacctt tctttctact taaatcccaa
    26761 tctttaccac tacctatatg gtctgtcaat gatctgtatt tttcctttgc gaatattcca
    26821 accttagtga ttcctgaatt tatcatatta gataaagcga agtctattat tctatatctt
    26881 ccagctattg gtgtagatgc tattggtctt ccatagttaa gtttgtttat tgctgggtct
    26941 ccttttttgt ttaaatttat tatccctaaa cattcatttc tcatcttaaa gacctccttc
    27001 tattaatacg catctaggtt ctatattttc atactcagaa actacattta tttcattatt
    27061 tacattcttt atctgaacat tatcatttat agttgcacac tcacctatca tagctttttc
    27121 tatagttaca ttttcaccta ttacaacatt tggcattatt actgagtcaa ttactttact
    27181 tcctttacca acaacaactc catgtgatag tacagaatta cctacttcac ctaaaattct
    27241 acaaccatca gctatcattg atctatgaac atttgcattt ttacctatat attgtggagg
    27301 cattgccata gtatttgtat atatcttcca tttagggtct gctaaatcaa gtgtttctgg
    27361 agattttatt atatccatat tagcatccca taagctttgt atagttccta catctctcca
    27421 gtatccttta aatggatagg catacattcc aacattatct tctaacattt taggtattaa
    27481 attttttcca aaatcatcat aatttatttc cggatttttt tctgcttcct taaaataatt
    27541 tctcaacatt ttccaatcaa atatgtatac tcccatagat gccaaattac tcttaggttc
    27601 acttggtttt tcttcaaatt catagatact tgaatcttca tttgtattca ttatcccaaa
    27661 tcttgaagct tcatcccaag gaacttcaat tactgctatt gtagctttag atcctttttc
    27721 cttatgaaat tttaacattt tactataatc catcttatat atgtggtcac cagataatat
    27781 caatacatat tctggattat aggtatctac aaagtccata ttttgatata tcgcatgtgc
    27841 tgtaccatta taccaatttc cttctttttc attcataaat ggttgtaaaa catagacccc
    27901 accatttatt ctgtctaaat cccaatgact tcccatacct atatgtgaat ttaatatcaa
    27961 cggtctatat tgagttaaaa cacctacagt atcaatacca gagtttgaac aattacttag
    28021 cacaaaatct ataattctat acttacctcc aaatgataca gctggtttag caattctttt
    28081 tgtaaagacg cctaatcttg aaccttgccc tcctgccaaa atcatagcta acatctcttt
    28141 tttcatttaa tgacactccc ctcatctagt aattagtatt tataattgca tttttacttt
    28201 tataaacttt atcagtcaat aaatgttgtg tataaatgtt gcttaaataa taccttaata
    28261 aaaataaaat aatatcaact atacaattga tacaatgtat accccataat gcacttgccg
    28321 acgtatacat tattttccat aatcactata gtattaatta atattcaatt atcagttaaa
    28381 aaataataca aattttattt tataaatata aaaacatttt ttataaatct cttgtgccat
    28441 acaaacatac ttgtttttta atttgttaaa ttcataatag caaaatacaa tctaacactt
    28501 gttaaataat tatccaaata aaatcaagta attattaatc aattttattt ttaattgata
    28561 cttctttgtc tatcatttaa acaagaatat ataaactttt agattattta ttagttttct
    28621 tattttaata tatatttttt tataaatata tattaataat acaaatagac taactaaaac
    28681 catgcttatt tgctatatgt tttatataat attatataac actttttaca ttagtaaaga
    28741 ataaattgta taaaaattct gttaattcat ctttataatt tcatctttat aataaaaaca
    28801 aagaagaaga cttaaaaatt tataagtcct cttcttttca acgtgcattt ttttactata
    28861 tttaattaat tcgaccttta atttccatat ccttcctatg ataacaaaac tcttttattt
    28921 ttttaatgat tcattatcac ctaaagcttc ttttacgtca aatatttctt tgttatcatc
    28981 actcataact tcttttattc ctttgaatgt tcctaagaaa ttaactgctt ctgaaggtaa
    29041 aaccagtttt gttgacttac cttcagccat tttttcaaga gcttccatag attttaatgc
    29101 tagaatatta ttatctatat cagcctcttt cattgctgaa aagactagtt ttaaaccttc
    29161 tgctgttgct atctgagttc tttttatcat ttcagcttca ccttgtgcct tagcaatagc
    29221 tgtctgtctt atagcttcag cctcaccttc tgccactaat atagcagact ctttttcacc
    29281 ttctgctaca cgtaccatag cttctttttt agcttctgct gttaatatag cagattgttt
    29341 ttcaccttct gcttgaagta ttgcagctga cttattacct tctgcttgaa gtattgcctc
    29401 tcttctttct ctttctgctc tcatttgttt ttccatcgca acttgaatat cctgaggagg
    29461 cattatattt tttaactcta ctctatttac ttttataccc catttatctg ttgcttcatc
    29521 aaggatagtt ctcattttta catttattat atctcttgat gttaatgttt catctaaatc
    29581 aagttcacca attatatttc ttagtgttgt agctgttaaa ttttcaatag cagctattgg
    29641 attagctatc tcaaacacat atctaactgg gtctgtaact ttataatata caactgtatc
    29701 aatctgcata gttacattat ctttagttat aactggttga ggtggaaaat ctattacaat
    29761 ttctctaagg tcaattacat atgccatttt atctaaaaat ggtattaata aatgtacacc
    29821 tgtttcagcc actttttgaa acttaccaag tctcattatt atacctactt tagattgttt
    29881 tataactctt atacaggtta aacttattgc tacgactact actattaata caatacttaa
    29941 aacaatttta gtacccatac tataaatcct cctttattct attgatttgt cacactagtt
    30001 gtagcgttta cttttttaac aactagtttt accccttcaa tttttaatac ttctactgta
    30061 gaaccttttt ctatcttttc accatctata gaaatagccg tccatgtttc atgttcaact
    30121 acaactagac ctgtattatt tggagaaatc tcttctgtaa caataccttt tttagacatc
    30181 attgcttgta aattagtgtt agatttgtag cctttatctt tttttactat ttttttagta
    30241 gctatgacca acattgctat agaaattact gcaaatatta aaatttgtaa gaaaatactt
    30301 tctataaaac tacttaaaaa tataagtact acagctccta cactaaacca tatcaatgtt
    30361 aagcttggtg tcaacatttc tgctattcca aataagactg ctactatcaa ccatattaac
    30421 ttcatactat ctaccctctt ttcatatgaa ttataaaacc ctatataaat tatatgttta
    30481 tacaatgttt ttgaatataa atttaaatta caaaacaata aaataaatta attaatagta
    30541 ttatatcaac atactttgtt aaagttgtta caaagtagta tcaaatatca ttaattatat
    30601 atccaacaac tctttcattg ttaatgaaat gttacttatt aaatccgtag catttactgt
    30661 atacattgtt ttactcaatt catctcctat atagccatgt atatatgctc cactaactgt
    30721 agcttccaat atatccatac cttgacctat aaatgaagta atcattccaa gcaaacaatc
    30781 ccccattcca ccagttgcca tacagctatt tcctgtagga tttatatatg tttttttacc
    30841 atctgttatg acagtttgat aaccttttaa aagcacaacc acattatatt tttgagcaaa
    30901 ttcttttgca acatctactc tatgttctct tatataccca attgaatctc ctgtcaacct
    30961 ggccatttct cccaaatgag gtgtaattac aaatctattt ttccattcta aaaacttata
    31021 acatctgtcc ttcataacgt tcaatccatc agcatctaat actatcgggc aattagaatt
    31081 ttcgactatc ttaagtaatt tatcaaatgt ttgactattg tttcccattc ctggaccaaa
    31141 accaattgca tcactagaac taagtaattt acttaatctt tcctcttcaa aatttaaagt
    31201 cattgcttct gtaagtctaa tagatacctt ttcttgcaca tcttcactac taattaaagt
    31261 tacaagtcca cttccactct taacagcaga ttcagttgct atgaaacttg caccataaaa
    31321 tccttttgaa cctgcaacta tagatacttt tccaaaatca cttttaaaac catgcttctc
    31381 tcttcttcta atattatttt ttacaaaagt cttatcagtt atgaactctt catcatggta
    31441 ttttcttgta ataaaatctg gaacacctat cttttctaca actacatcac ctataagtgc
    31501 gctagtctca tagtttagaa aacctctttt ataaaactca aagctaatcg ttttgtttgc
    31561 tctaatacaa gttcctaata catttccatt atcgctatct atacctgaag gaatatctat
    31621 agaatatgta tttttgctat tatcgtttac tatgtcaata acttctttaa aaataccagt
    31681 tatatcacgt tttagtccag tcccaaatat agcatctact acaacatcac ttttagatac
    31741 tctatcttta aaaattgtta aatttttatt ttttataata ttttgtttct caactttaat
    31801 aattttatct acttcatttt catctccata atttttttca cttaaatcat ttttattaat
    31861 atttataata ttaattccca tatttaaaag tacattgtaa tttattttag aacattcact
    31921 tagtttttca atattaccta ctaaaaatac gtttacttct tttccacatg tatttagatg
    31981 tcttgctatt ccaagaccat ctcctccatt atttccaaca ccacatacaa ctgtataact
    32041 attatacatg tctatatcca tatttttcat tgcagatata actgcatttt ccatcaatac
    32101 aatcattgga atacctactt cttctacaca tgcagtatca acatttctac ttaacttaga
    32161 tgtacctatt agcataaatt atcactccta taaagttaaa gacttcttaa atattcataa
    32221 aaaaatatct attgaaatct ttctaagcac ataaatatca taaataaaat cactaaaact
    32281 aatatttttg aaataattta aaataacaaa aaattttgaa ttaaagtaat tatttaattt
    32341 attataccat ataaattaag ctattatgtt gatttaaaac ctatttacac taattatcta
    32401 aatatttctt tattttcata atcttttatg acttatttat atttttcatt tttataaaaa
    32461 aacaataatt aagaatataa ctatattcta cacataaaaa tttttttgtt taaacttaat
    32521 tttctgataa tttgataatt tttcaaaatt tatagttgac ttttattttt tactgtatta
    32581 aactaattac tatacaaaac ggaaaacaaa attttcaaaa ttattaaatt aaaaggagag
    32641 gtaaatatga agactttatt aaacaaacaa gtacgatttt ttagtagcca atttagttag
    32701 ggtttgtacc taagttaaga cgaattgtca taacatagat acaagcccct taggattatg
    32761 atttttaagg attgtatcta tgttggctat agtaattgtt gttaaagttt ttattttatc
    32821 atcataatat agagccacat ggattatact gtgtggctta ttcgtatttt tgccatacag
    32881 ttgtatatac tgtgtggttt ttttatatca aaattgagtt ttcaaattaa attcaaaaat
    32941 ttatttaaaa tattttcaaa gg
//
LOCUS       DAFNNB010000027        31685 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0027, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000027 DAFNNB010000000
VERSION     DAFNNB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31685
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0027"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            88..843
                     /gene="nadE"
                     /locus_tag="L3M60_001109"
     CDS             88..843
                     /gene="nadE"
                     /locus_tag="L3M60_001109"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HBN6225798.1"
                     /translation="MSNIKIQIDKTVEWLINKVNEANAKGLIVGVSGGIDSAVVANLI
                     KKAFPENSMGVIMSIKSNPQDREDALKVIEGCDIEYLDLDLIEPQSAILDMVVGNLKD
                     KHLYREEYLKMTDANLRARVRMSTIYTIANNLGYLVVGTDNAAEIHTGYFTKFGDGGV
                     DILPIANLTKGEVYEWAKELGVHEDLINKAPSAGLWEGQTDEDEMGTTYNMIDAVLEG
                     RLDEVPKRDQEIIERLHRLSEHKRKTPSQPPKF"
     gene            926..1645
                     /locus_tag="L3M60_001110"
     CDS             926..1645
                     /locus_tag="L3M60_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN6225799.1"
                     /translation="MGRIGNIINRKGKQDAQRAKIFTKHARAIAVAAKDGGADPEYNA
                     ALKTAIEKAKADNMPNDNIDRAIAKGAGAGAGEDYETIVYEGYGPGGVAVIVETLTDN
                     KNRTAGNVRYYFDKNGGNLGTSGCVSFMFDKKGQILVGLGDGVSEEELMDVALEAGAE
                     DFITEEDGYEIITTPEDFSSVRDELKAKGYEFISADVKMIPQTTTVLTEESHLKMMNK
                     LVDMLEEDDDVQDIYHNWEVE"
     gene            2389..3183
                     /locus_tag="L3M60_001111"
     CDS             2389..3183
                     /locus_tag="L3M60_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HBN6225800.1"
                     /translation="MESIMENLNNLKVEVKDKVCVITINRPKALNALNNDTLRELSQV
                     IDVISENEAILGVIITGEGKVFVAGADIRQMQNYKSEEGRKYAGYAQGIFDKIEALEK
                     TVIAAVNGYALGGGCELAMSCDIRIASEKAIFGQPEVNLGVIPCFGGTQRLSRLVGTG
                     IAKELIFTGRQVNAEEAKSIGLINKVVPSDLLLEESIKMMNQIVEKAPIAIRYAKVVI
                     NKGIDMDLKSALELEKDIAGLTFATRDKQEGMNAFIEKRKPVFENK"
     gene            3277..4617
                     /locus_tag="L3M60_001112"
     CDS             3277..4617
                     /locus_tag="L3M60_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBN6225801.1"
                     /translation="MNTGVIISLVGIIIALSLLVVMVMKGINIFIIAIVCSSLVAITG
                     SLNVYDALKVDYMTGFVGFLQANFFIFLTGTLMGKIMEITGGAKSIAKMIVRWIGKDK
                     ALLSIPIACGILAYGGVSVFVVSFAVFPIALEIFKEADLPRRFIPAALTFGCSTFAMV
                     APGAPQIQNIVPASTLGTDIMAGAVNGFISCAVMFVVGSTILYKMVSKEKANGGHFIA
                     HESDVFDESEATNSKKENGPNGVIALIPLIISILIINVKINGQAIVPIEVGVFIGALL
                     VYLLLNKYQDNAKIVGHVGDACKTTVVAICNTCAVVAFGSVVKSAVGFDFIVNAMTNI
                     PGPPIAGAAVGTTVIAGICGSASGGLGIAAPLLGPVFLAKGVSAAALTRTMAISSAAL
                     DSLPHNGYIVTVTNGLCKETHKDAYMPIFWVTVLTPFIGTIVAVLLFTMFPGLP"
     gene            4754..5986
                     /locus_tag="L3M60_001113"
     CDS             4754..5986
                     /locus_tag="L3M60_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HBN6225802.1"
                     /translation="MLNKPLEGVKVIDLTYFVAGPGTSKILADWGADVIKVEPSFGDP
                     GRKTGATMTMPIDDYNNPFYSTYNSNKRGLSINLKSKAGIEIMDKLLSEANVFVSSYR
                     TGALKRLGLDYESLSKKHPHLIWGQINGFGDFGPAKDNAGFDTVAFWARSGAMLDIAE
                     KDTSPINPPIAFGDATTSCSLSGGICAALYQQAKTGKGQKVMASLFGQAIWNASSLLA
                     STQFSDEYPKTRKNAISPVINSYRCKDGKWIFLSILEHERYFEALCRLFGREDLIKEE
                     KFASSVESKKNATELINILDGEFAKFTQNEMVEKLTAADIAHEKIQHVNDVVTDEQAI
                     ANNYIYEHTSKNGNKTMLASTPVKFGNIEVNMTCDAPLIGEHSDEILRELGYSDSDIE
                     ALIESGIVTMNKEACEIC"
     gene            6026..7168
                     /locus_tag="L3M60_001114"
     CDS             6026..7168
                     /locus_tag="L3M60_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HBN6225803.1"
                     /translation="MDFRLTEAQLMLQRVAKEFAENEIAPIAAETDKTGIFPRELFSK
                     MAKIGFNGIGTPVEYSGSGGADIEKVIVVTEIAKKCAASAAILSIHTIYAQAILKFGT
                     EEQKKKYLPMMAEGGCVGAFALTEPNAGSDAARAATTAIIDEETDEYVLNGTKCFISG
                     GGQAESLIIFALTDPSKGIKGMSAIIVDKGTPGFSIGKIEEKMGIHGSETAELIFDNC
                     RVPKSNLLGKEGKGFNIAMTCLDGARIGVGAQAVGIAEGALEESIKYSKERVQFGKPI
                     SALQGIQWYIADMATMVESAKLLVYYAADLKARGEKHTKEAAMAKYNASHTAREVTNL
                     ALQIHGGYGYMKDYPLERMYRDAKITEIYEGTSEIHKVVISRAVLG"
     gene            7179..7973
                     /locus_tag="L3M60_001115"
     CDS             7179..7973
                     /locus_tag="L3M60_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBN6225804.1"
                     /translation="MNIVVCLKQVPDTNEVKINKETGTLIRDGVPSIINPDDRNALEE
                     ALKMKDELGAVIKVISMGPPQAKSALKEALAMGADEAYLISDRAFGGSDTWATSTIIA
                     AAIEKVGKYDVIFCGRQAIDGDTAQVGPEVAEFLGIPQVTYAKEVKVQDDKLLVTRYT
                     ETGDYLIEAKMPVLLTAIKELNNPRYPSVKGILEAYNNGDAKINVLTLADLDVDTTQI
                     GLKGSPTNVYKSFVPVKDKHNEIIEGINKKEKAEKLIEILFDLKLV"
     gene            7984..9006
                     /locus_tag="L3M60_001116"
     CDS             7984..9006
                     /locus_tag="L3M60_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBN6225805.1"
                     /translation="MMRAKVNQGINLNDYNGVWVIGEQREGKINPVTIELIGEGRKLA
                     DQLGKELAVVIAGYEVEKEVKELLHYSVDKVYYINDPLLKDFTTDGYAISIANLIERK
                     KPEVVLVGATSIGRDIAPRIAGKVGTGLTADCTKLEIDSTDNKLLQTRPAFGGNLMAT
                     IVCPKNRPQMSTVRPGVMAKAVRNESETGILEVVTPELTEKMIRTRLVEILPQEKKSV
                     NLTDARIIVSGGRGLKRAEGFELIKELADKLGAEIGASRAAVDSGWIEHSHQVGQTGT
                     TVRPELYIACGISGAIQHLAGMSDSKYIVAINKDAKAPIFSMCDYGIVGDLYEIIPEM
                     IESLNR"
     gene            9105..10481
                     /locus_tag="L3M60_001117"
     CDS             9105..10481
                     /locus_tag="L3M60_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBN6225806.1"
                     /translation="MKEQWYKDIFARVLSMTDDGFIVVNTSGVIIDINDKYCDFLGKE
                     RKDIIGHSIQSIIPNTKMLDVMRDKYCEEGAIHHYSGGNTKEKSVIVSRSYVENDNGE
                     VVAGVAQVKFRLQSFDVAKKLMSEYMELQYYKEQFKDNCGFDKLIGENKDFIELKKTG
                     VKASKTNFPVLLTGETGTGKEVFARAIHNNSSRSDKPMVSINCAAIPEELLESELFGY
                     DEGAFTGAKKGGKKGKFLVANNGTIFLDEIGDMPLTMQAKLLRVLQESEIEPVGGLKT
                     IKIDVRVISATRKNLSKMVEEGLFREDLYYRLNVINIHMMELKDRQDDILLLANYILN
                     KLNVEYKELKVLSDKVKNCFINYTWPGNIRELQNVIKSAYAVSDDMVIMMCDLPSKMD
                     NISRVAQCNVDSNCSIHEMVENYEKSLIIDVLRKYNWKCSKAAEVMGIHKSLLYKKIK
                     KYEIELNN"
     gene            complement(10812..11156)
                     /locus_tag="L3M60_001118"
     CDS             complement(10812..11156)
                     /locus_tag="L3M60_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225807.1"
                     /translation="MDQIKRLHELQQKSYLTKEEFEELKNNTYIEKFELRDECDNSYI
                     YTFYTNDKCNDIKFIKSQFIVTLIKIGFLCECGGVFRQKKIVDEYDYGYNAIYKCDSC
                     GKELDKEVENYD"
     gene            11644..12654
                     /locus_tag="L3M60_001119"
     CDS             11644..12654
                     /locus_tag="L3M60_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBN6225808.1"
                     /translation="MHEDKNDIFESIDTEDFDNKTEEECLELVRKNGLNLIYIKNQTE
                     EICLEAVRQNCNAIKCVQNQTEKICIEAVKQDWRMLEFVEEPTEEICMLAINQDGTAL
                     KYIENQTEELCLRAVEKNGTALEYVKEQTEEICIEAVRNSEFGLARVKNQTEKICMEA
                     VKHCSYNLKWVENQTEEICMEAVRQNGLDLKFVKNQTETICLRAVRQNGLALEFVKEQ
                     TVGICLKAVRQYGMALKFVKEQTEEICTEAIKQDKRALSFVKGDKEKYKALCDNNEPF
                     AKRYVRNVIEKENRALIKKGEENAKIKIAGKLYDRGMSFEDISDIVEIEISKLKVSLG
                     IF"
     gene            12790..13278
                     /locus_tag="L3M60_001120"
     CDS             12790..13278
                     /locus_tag="L3M60_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017825269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6225809.1"
                     /translation="MKITILYQTRSGNTERVAKLIEDGAKKVDGVDVKLMRLDNIDLD
                     YLNESKAVIFGTPTYLANTTWEVKKWFDEDSKKVNLAGKLGAVFATCDYICGGPDVAI
                     LTVVGHLMVKGMLVYSGGGSLGKPFIHLGHVHSIEGPELQDEKAIIFGERIATKAKEL
                     FN"
     gene            13341..15269
                     /locus_tag="L3M60_001121"
     CDS             13341..15269
                     /locus_tag="L3M60_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6225810.1"
                     /translation="MVWHIEYEVFSAIIVIFLMVYFFRSKFIPTLQNKIYCALLIFSF
                     LFIISNILGSFCLNNIEKIPIFITFLLNQIYSLLLPIPAALVSFYVMAIIYQDIRYMK
                     KRLLFLSIPLLICIILSITNPFTHILFGLSKETGYIRGCGYVATFSSFFFYAIYSALL
                     SFKYRKAMHESKIWAIRGFLIVSIVAIILQAIFLQYLLTGTACACSLLLTYLSMQNRG
                     LIIDDLTGVLNRQSFIQELDLNINTEQSGFIITIALDDFKFMNETFGTKNGDIALKEV
                     GKYLVHIHDVNHVYRIGGDIFSIVVNSQLGIKPEDLIKKIEERFKKTWLVEEISFNLA
                     TSIAVVYYPENAETTEDVIVAIDFSIHEAKRSESRRVVYADASISEKIKRKHVIKNCL
                     EEALKNDGFEVYYQPIFSNSRGRFTSAEALLRLEHKELGFIPPDEFITIAEQTGLINL
                     IGLVVFEKVCRFIASDEFEGLKLDNIAVNLSVVQCMQKNLADDLLYLMDKYNISPTKF
                     KLEITETVAMGSFNIIKETMERLIDLGVKFALDDFGIGYSGVSNMLSLPFSVIKLDKS
                     LIWSMNEDSRHKLTVETIIALINKLNMKAVAEGVETIKCAEDLIAMECEYLQGYYFSK
                     PIPEDAFKNLLKENAFTG"
     gene            15468..15878
                     /locus_tag="L3M60_001122"
     CDS             15468..15878
                     /locus_tag="L3M60_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HBN6225811.1"
                     /translation="MNKKKILVPIDGTERSMHSLEFIKGIFKKDEVEVEIMNVKELVF
                     IDGISLAEEIKNSENLGRRILDKAAEIMGEYDVKVHFTFGYPGDEIIRKAKEDNVDFI
                     VMTKSTKKGLTRMIGSVTASVVKQAKCIVMIVPE"
     gene            15900..16202
                     /locus_tag="L3M60_001123"
     CDS             15900..16202
                     /locus_tag="L3M60_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HBN6225812.1"
                     /translation="MNKKIVAVCESERSLNSLKMAANDLGFDLVYEIQENDNIKNELS
                     IYDIEDASIVLFVTSDSIESIEKIDRFIDREFYEVDPKYIIEDAKSIVSEILIDLN"
     gene            complement(16374..17069)
                     /locus_tag="L3M60_001124"
     CDS             complement(16374..17069)
                     /locus_tag="L3M60_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBN6225813.1"
                     /translation="MKSLNATEKIIDYIKLNILNGTFKINSKLPSERKIADLFNISRI
                     PVRNAINILCKEGILRAVPYSSPIVEGFKKIDLFDDGEIYKNHNIQEFYVESLRARQL
                     IESEATRLAILNATSKEIQKIKYTYLKSIEELDKVSQGLIEECYDADLQFHKEIILAS
                     HNPIFIRYYELIPKTISSNQYFGFKYRNSLKDMISHHNNIIIAFDSKDSNLGYTSMYN
                     HLEDVIQLFQHDD"
     gene            complement(17095..18282)
                     /locus_tag="L3M60_001125"
     CDS             complement(17095..18282)
                     /locus_tag="L3M60_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6225814.1"
                     /translation="MKKTSNQKLLLLSIVTFFWFSQYVYIPFQSPYLSACNVSTNMIG
                     IIIGAYGISQCLLRLPVGVFADSINKHKIFILSGIFFAGLASLIRVFFPEGNGFLLAN
                     ILSGISSSTWISYMVHYTNFFSKEHQQKATSTIILVNNLGMLIGFVMSTLFYEKLGMT
                     ALCIMSVLSSVIGTILALNIKNHNSKKMPKKISIKKYLSICKDKRLIFFSFMALIQQG
                     IQMSTTMSFTTQILKNLGSSSTLIGLSSIFYMVCAVISSGFASTKFCTKYEPKFWIPL
                     VFILVSAYCFLVPTIKNINIIFVLQVLPGISTGILLSYLTSESMKNVPKHQRSTAMGF
                     FQAIYAIGMSVFPMIVGKISEYTSIRYGYFVLAIIAIVASILSYTYYKKLDLTLNYDI
                     IQS"
     gene            18618..20495
                     /locus_tag="L3M60_001126"
     CDS             18618..20495
                     /locus_tag="L3M60_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6225815.1"
                     /translation="MDYKNIAQEILLNVGGKENVNEVTHCMTRLRFKVKSASKVNKDK
                     LSKIEGVITVVESMGQIQVVIGNKVKKVYDEVIKIVPQSNEVGKKNESEEKQGIINSI
                     LSAVAGIFTPTIPAIAGVGMIKGILSVLAMYYMNKNGVDIKETQSYIILNAMADSIFY
                     FMPIILGYTAAKVFNANKIISMVLGATLCYPTFTALMAGEEAVRFLGLAVTKATYTSS
                     VIPIIIAIWALSYVEKVLEKYIPEVIKIIMVPTLSLVIMLPATLFLFGPIGIYIGNII
                     NFSYKYIYELSPALCGAFVGGLWCVLVIFGAHRALLPIGISDVAQTGRQNLLAFAGAA
                     NFSQAGAALGVFFKTKNQGLKTISMSATITALFGITEPAIYGANLRLKKPMVCAVICG
                     AVGGAIMGMGGAYGNAFANQGVLTIPVYAEAGALGFLSYLGGCAIAFFGSAISTYLVG
                     FEDLEESSNKENSFIKVETKDGAIDITSPVEGECIELSEVKDDVFASKAMGEGIAILP
                     TKGVITAPTDCEVASLFPTLHAIGLKLDNGAEMLIHVGINTVELNGKYFTKHVNQGDL
                     VKKGDKLISFDIDKIKKAGYDVTTPVIVNNTFDFGQVVSCKSSYVSTNDNIISLVLAG
                     N"
     gene            20736..22142
                     /locus_tag="L3M60_001127"
     CDS             20736..22142
                     /locus_tag="L3M60_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBN6225816.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYNNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGIDFYNKII
                     DKLIECDIEPFVTLYHFDLPFTLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMRYLEKRNIVPHM
                     EDGDKEVLKKAKMDFIAVNYYRTNCAGAFPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACRLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            22170..23018
                     /locus_tag="L3M60_001128"
     CDS             22170..23018
                     /locus_tag="L3M60_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6225817.1"
                     /translation="MYITKVLNNSLLLARDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLELPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSKEKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            23678..24370
                     /locus_tag="L3M60_001129"
     CDS             23678..24370
                     /locus_tag="L3M60_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225818.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEHIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKNDNYSKVHGLRAGADDYLTKP
                     FEIAEFTARVLSLIRRYTRFNPKNEESQQLSFKGLLIDLDQHTVTTNGETMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            24367..25302
                     /locus_tag="L3M60_001130"
     CDS             24367..25302
                     /locus_tag="L3M60_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6225819.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYIEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLDFEIDIPENRLMVNLDSDGYSRVVNNLVQN
                     VLAHSKARRIKITMSEDSKMVLLRVEDNGVGIAKENLQHIFERLYKCDKGRSKKGSGL
                     GLSIVSQMVERMGGKISVESEIGKYTVFIVSLPLI"
     gene            25452..26375
                     /locus_tag="L3M60_001131"
     CDS             25452..26375
                     /locus_tag="L3M60_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225820.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGSGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRNLCTERGKTILISSHILSEIALLADDIGIIDRGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLEIQLNLKNFKVADNHNIMLYDTDVSVAEINRAF
                     IQNNIDVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            26359..27129
                     /locus_tag="L3M60_001132"
     CDS             26359..27129
                     /locus_tag="L3M60_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225821.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIVFGIMFFVTALPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICIIYKKQEN"
     gene            27139..27921
                     /locus_tag="L3M60_001133"
     CDS             27139..27921
                     /locus_tag="L3M60_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225822.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQIVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFVITLIGGYLINREYTDQTLKNILTIPISLRKLLVGKL
                     IAVGGITVLLSIFSFLCTFILGLLFCNINISVILVAKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNSFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLAGYNNDDGSVYQPLI
                     GVMTLVVILILTAILLTFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            28299..28835
                     /locus_tag="L3M60_001134"
     CDS             28299..28835
                     /locus_tag="L3M60_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBN6225823.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            28867..29256
                     /locus_tag="L3M60_001135"
     CDS             28867..29256
                     /locus_tag="L3M60_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBN6225824.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLSQLTEHGFIKKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLKELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            29293..29679
                     /locus_tag="L3M60_001136"
     CDS             29293..29679
                     /locus_tag="L3M60_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBN6225825.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNSVTVKVSSAIHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            29806..31248
                     /locus_tag="L3M60_001137"
     CDS             29806..31248
                     /locus_tag="L3M60_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBN6225826.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDENIAEDIVEDEPKLNTEKDGDLNYVSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAKSKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGKDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            31545..31664
                     /locus_tag="L3M60_001138"
                     /pseudo
     CDS             31545..31664
                     /locus_tag="L3M60_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT        11785 a         3714 c         5972 g        10214 t
ORIGIN      
        1 tttgcataaa aaaaacatat taaaacaaca tattaatata tggtataata tattaaaaca
       61 aagtagcaaa tttgagagga gataacaatg tcaaatataa aaatacaaat agacaaaact
      121 gtagaatggc ttataaataa ggttaatgag gcaaatgcaa aaggattaat tgttggtgta
      181 tctggaggaa ttgattcagc agtagttgct aatttaatta aaaaggcatt tccagaaaac
      241 tctatgggag ttataatgag cataaaaagt aatcctcaag atagagaaga tgcacttaag
      301 gttatagagg gatgtgatat agaatatctt gatttagacc ttatagaacc acaaagtgct
      361 atattagaca tggtagtagg aaatttaaaa gataaacatt tatatagaga agaatattta
      421 aagatgacag atgcaaactt aagagcaaga gtaagaatga gtactattta tacaattgct
      481 aacaatctag gatacttagt tgttggtaca gacaatgctg cagaaataca cacaggatat
      541 tttactaaat ttggtgatgg tggagttgat attttaccta ttgcaaattt aactaaagga
      601 gaagtgtatg aatgggcaaa agagcttggt gttcatgaag atttaataaa caaagctcct
      661 tcagcaggtc tttgggaagg tcaaacagat gaagatgaaa tgggaactac ttataatatg
      721 atagatgctg tgttagaagg cagattggat gaagttccta aaagagacca agagataatt
      781 gaaagacttc atagattaag tgaacataag agaaaaacgc cttcacaacc tcctaagttc
      841 taatttacta tttataagat ttgtgataaa atttacaagt gatgaaaatt agaataatta
      901 attgtacgta ttaaggagtg gaaatatggg acgtataggt aatataatta atagaaaagg
      961 taaacaagat gctcaaagag caaagatatt tactaaacat gctagagcaa tagcagttgc
     1021 agctaaagat ggtggagcag acccagagta caatgcagcg ttaaaaacag ctatagaaaa
     1081 agctaaggca gacaatatgc caaatgataa tatagataga gctattgcta aaggtgctgg
     1141 agcaggtgct ggagaagact atgaaactat agtttatgaa ggatatggac ctggtggagt
     1201 tgctgttata gtagaaactt taactgacaa taaaaacaga actgctggta atgtaagata
     1261 ctattttgac aaaaacggag gaaacttagg aactagtgga tgtgtgtctt ttatgtttga
     1321 taaaaaagga caaatattag ttggtttagg agatggagtt tcagaagaag aattgatgga
     1381 tgttgcatta gaagctggag ctgaagattt tataacagaa gaagatggtt atgaaataat
     1441 aactacacca gaagattttt ctagtgttcg tgatgagtta aaagctaaag gatatgaatt
     1501 tatatctgct gatgtgaaga tgatacctca aactacgact gtattgactg aagaatctca
     1561 tttaaaaatg atgaataaat tagttgatat gcttgaagaa gatgatgatg ttcaagatat
     1621 atatcataac tgggaagtag aatagatagt ataagtatat tgaagtttat aagtattgca
     1681 ttaattttaa tatgtgataa attttataac atattaaatt actaaaataa acatatacac
     1741 ctctaaccta tatttgcata ataggtaaga ggtgttttta tatttatagg ttaagaaatt
     1801 taaaagaaaa cggagttatt tgatttagaa taatatcaaa tgagattaac ttaacattgc
     1861 aaatcaaatc taaaattgtc agaaacgttt aagatgggaa aagttactta gacattaagt
     1921 ctttgtggtt tttcctattt ttattgataa atttgtacta ttagaataga tatatattga
     1981 actaaagtat taatgattta agtcaataga aaataaatta tctactatgt tgaataaaga
     2041 ttccttatat tagagtgaag taaacttata gatatactgc ttaaaatatt ttaatagaaa
     2101 tatcaaaaat gagactgcat tctcaaaaat gagactgtgt tttttaggta ttttctcgaa
     2161 aatgagacaa aaatattgtt ttatgagttt tattgaagaa aaattctttt ttgaattaca
     2221 atttaatcaa ttttaaagtt aaaaattatt aaaaatttag tgaaatttat gtatatttcg
     2281 tttattttat taaaaataaa gagcttcact ggtgattggc atagtacttg ctattttata
     2341 ttaacaagaa atgtagaaaa aataacaatg tataatgggg ggaatatgat ggaatctata
     2401 atggaaaatt tgaacaatct taaggtggag gttaaggaca aagtctgtgt tattactata
     2461 aatagaccca aagctttaaa tgctcttaat aatgacaccc ttagggaact atcacaagtt
     2521 attgatgtaa tatcagaaaa tgaggcaata ttaggagtca ttattactgg agaaggtaaa
     2581 gtttttgtag ctggggctga tatacgtcag atgcaaaact ataaaagtga ggaaggcaga
     2641 aaatatgcag gttatgctca gggaattttt gataaaattg aagctcttga gaagacagta
     2701 atagcagcag ttaatggata tgcattaggt ggtggatgtg aacttgctat gagttgtgat
     2761 attagaattg catcagaaaa ggctatattt ggacaacctg aagttaattt aggagttatt
     2821 ccatgttttg gtggaactca aagattatca agacttgttg gaactggaat agccaaagag
     2881 ttaattttta cggggagaca agtaaatgca gaagaagcta aatctattgg gcttataaat
     2941 aaagtggttc caagtgattt acttttagaa gagtctataa agatgatgaa tcaaatagtt
     3001 gaaaaagcgc caatagcaat aagatatgca aaagttgtta ttaacaaggg gatagatatg
     3061 gatttaaaga gtgctcttga actagagaaa gatattgcag gtttaacttt tgctacaaga
     3121 gataagcaag aaggaatgaa tgcatttata gaaaagagaa agccagtttt tgaaaataag
     3181 tagctattac aactaaattg gaacaatgta gaatatattc aaaatctaaa tttcgtgaaa
     3241 aaatacatat agtttaattt taaaaggagt taatttatga atacgggagt aattataagt
     3301 ttagtaggga ttatcatagc tctatctctt ctagttgtaa tggttatgaa gggaatcaat
     3361 atttttataa tagcaattgt atgttcatca ttagttgcta taacaggaag tctaaatgtg
     3421 tatgatgcgc ttaaggtgga ttatatgaca gggtttgttg gatttcttca ggcgaatttc
     3481 tttattttcc taacaggtac attaatggga aaaataatgg agattactgg tggagcaaag
     3541 tctattgcta agatgattgt aaggtggatt gggaaggata aagcattact ttctattcca
     3601 atagcttgtg gaatcttagc ctatggtgga gtaagtgttt tcgttgtaag ttttgcagtg
     3661 tttccgatag ctttagaaat ttttaaagaa gctgatttgc cacgaagatt tataccagca
     3721 gcgttgactt ttggatgttc aacttttgct atggttgcac caggcgcacc acaaattcaa
     3781 aatattgtcc cagcctcgac attggggaca gatattatgg ctggagcagt aaatggtttt
     3841 atatcatgtg cagtaatgtt tgtagttgga agcactattt tgtacaaaat ggtttcaaaa
     3901 gaaaaggcca atggtggtca ttttatagca catgaatcag atgtatttga tgaatcagaa
     3961 gcaactaatt ctaagaaaga aaatggtcct aatggtgtaa ttgcgttaat tccattaata
     4021 atatcaattt taataattaa tgtaaaaatt aatggacaag caatagtacc aattgaggta
     4081 ggagtattta ttggagcttt attagtgtac ttattgttaa ataaatatca agataatgct
     4141 aaaatagttg gacatgttgg tgatgcatgt aaaactacag tagtagcaat atgtaatact
     4201 tgcgctgtag ttgcatttgg ttcagtagta aaatcagcag ttggttttga cttcatagtt
     4261 aatgccatga ctaatattcc tggtcctcct attgcaggcg ctgcagttgg aactacagtt
     4321 attgcaggta tctgtgggtc agcttcaggt ggactaggaa ttgcagctcc acttcttgga
     4381 ccagtatttt tagctaaggg tgtttctgct gcagcattaa ctaggactat ggcaatttca
     4441 tcagcagcat tagattcact tccacacaat ggatatatcg ttactgtaac aaatggtctg
     4501 tgtaaagaaa cacataagga tgcctatatg ccaatattct gggtaactgt tttaactccg
     4561 tttattggaa ccatagtagc agtcttactg tttacaatgt tcccaggatt accataagaa
     4621 agaagataaa atgcataaat tatattttga tattgtaaaa ttaattttca aagtatatac
     4681 aataaatttc atgttttaat actgtaagtt tgtatttaaa ataacaaaat gaccatataa
     4741 ggaggaattg aaagtgttaa ataaaccatt agaaggagtt aaggttattg acttaacgta
     4801 ttttgtagca ggtccaggaa cttctaaaat attagctgac tggggagctg atgttattaa
     4861 ggtagagcca agctttggag acccaggaag aaagacagga gcaacaatga caatgccaat
     4921 agatgattat aataatcctt tttacagtac atataattca aataaaagag gtctatctat
     4981 aaatcttaag agcaaagctg gaattgaaat tatggataaa cttttaagtg aagcaaacgt
     5041 atttgtttca agttatagaa ccggagcatt aaaaagacta ggtcttgact atgaatcatt
     5101 aagtaaaaag catccacacc ttatctgggg tcagataaat ggttttggcg actttggacc
     5161 tgctaaagat aatgcaggat ttgatacagt tgccttttgg gcaagaagtg gagctatgct
     5221 tgatatagca gaaaaagata caagcccaat aaatccacct atagcttttg gagatgcaac
     5281 aacgtcttgt agtttatctg gaggaatatg tgcagctttg taccagcaag ctaagactgg
     5341 aaaaggacaa aaagtaatgg catctttatt tggacaagca atatggaatg catcatcttt
     5401 attagcatct acacagttta gcgatgagta tccaaaaact agaaaaaatg ctatttctcc
     5461 agtaataaat tcttatcgtt gtaaggatgg aaagtggata ttcttaagta ttttagagca
     5521 tgaaagatat tttgaggcac tttgtagatt atttggtaga gaagacttaa taaaagaaga
     5581 gaaatttgct tcttcagtag agagtaagaa aaatgctaca gaacttatta atattttgga
     5641 tggcgaattt gcaaagttta ctcaaaatga gatggttgaa aagcttactg ctgctgatat
     5701 agcccatgaa aaaattcaac atgttaatga tgtagtaact gatgaacagg ctattgctaa
     5761 taattacata tatgagcata caagtaaaaa tggaaataag acaatgcttg catctactcc
     5821 agttaaattt ggaaatattg aagttaacat gacttgtgat gctccactta taggagaaca
     5881 ttcagatgag atattgagag aacttggata cagtgattct gatatagaag ctctaattga
     5941 atctggaatt gtaacaatga ataaagaagc atgtgaaata tgttaataaa catgcggagt
     6001 atgttaataa ataggaggga attttatgga ttttagatta acagaagcac aattaatgct
     6061 tcaaagagta gcaaaagaat ttgcagagaa tgaaatagca ccaatagctg ctgaaacaga
     6121 taaaacaggt atttttccaa gggagctttt tagcaaaatg gctaaaatag gatttaatgg
     6181 tattggaaca cctgtagagt atagtggttc tggtggagca gatatagaaa aggtaattgt
     6241 tgttactgag attgccaaaa aatgtgcagc atctgcagca atattatcaa tacacacaat
     6301 atatgctcaa gctattttga aatttggtac tgaggaacaa aagaaaaaat accttccaat
     6361 gatggctgaa ggtgggtgtg taggtgcttt tgctttaact gaacctaatg ctggttcaga
     6421 tgctgcaaga gctgcaacaa ctgcaattat tgatgaagaa actgatgagt atgttttaaa
     6481 tggaactaag tgttttattt ctggtggagg tcaagcagaa tcattaataa tatttgcact
     6541 gacagaccca tcaaagggaa taaagggaat gtctgctatc attgttgaca aagggactcc
     6601 aggtttttca ataggaaaaa ttgaagaaaa aatgggtatt catggttcag aaactgcaga
     6661 gttaatattt gataactgta gagttccaaa gtctaactta ttaggaaaag aaggcaaagg
     6721 atttaacata gctatgacct gtttagatgg agcaagaatt ggagttggag ctcaagctgt
     6781 gggaattgct gaaggagcct tagaagaaag tatcaaatat tctaaagaaa gagttcagtt
     6841 tggcaagccg atttcagcct tgcaaggtat tcaatggtat atagcagata tggcgacaat
     6901 ggtggaatca gcaaaattgt tagtatatta tgctgcagac ttgaaagcta gaggagaaaa
     6961 acacactaaa gaagctgcca tggcaaaata caatgcatca catactgcaa gagaggttac
     7021 aaatctagct cttcaaatcc atgggggata tggatatatg aaggattatc cattagagag
     7081 aatgtacaga gacgccaaaa taacagaaat ttacgaaggt acttcagaaa tacacaaagt
     7141 tgttatttca agagcagtac taggatagga ggaagagaat gaatatagtt gtttgcttaa
     7201 agcaagttcc agatactaat gaagttaaaa taaataaaga gacaggtact ctaataagag
     7261 atggtgtacc aagtataatc aatccagatg atagaaatgc cttagaagaa gctttaaaaa
     7321 tgaaagatga attaggagca gtgattaagg ttataagcat gggacctcct caagctaaaa
     7381 gtgctttaaa agaagctttg gcaatgggtg ctgatgaagc atatttaatt tcagatagag
     7441 cttttggtgg ctcagatact tgggctacat ctacaattat tgctgctgct atagagaagg
     7501 ttgggaagta tgatgtaata ttctgcggaa gacaagctat agatggagat acagctcagg
     7561 ttggaccaga ggttgcagaa tttttaggaa taccacaagt tacttatgca aaagaagtaa
     7621 aagttcaaga tgacaaatta ctagtaactc gttatacaga gacaggagat tatttaatcg
     7681 aagcaaagat gccagtttta ctaactgcta taaaagagtt aaataatcca agatatccaa
     7741 gtgtcaaagg aatcttagaa gcttataata atggagatgc aaagattaat gtgttaacac
     7801 ttgcagattt agatgttgac acaactcaaa ttggattaaa gggttcacct acaaatgttt
     7861 ataagtcttt tgtaccagtt aaagacaagc ataatgaaat aatagaaggt ataaataaaa
     7921 aagaaaaagc tgagaaacta atagaaatac tattcgattt gaaactagta taggagtgtt
     7981 aagatgatga gagcaaaagt taatcaaggt ataaatttaa atgattataa tggtgtttgg
     8041 gtaataggag agcaaagaga aggcaaaatt aatcctgtta ctatagaact tattggtgaa
     8101 ggaagaaagc ttgctgacca attaggaaaa gagcttgcag ttgtgatagc tggatatgaa
     8161 gtagagaaag aggtgaagga attacttcac tatagtgttg ataaagtata ttatatcaat
     8221 gaccctcttc ttaaagattt cactactgat ggatatgcaa tttcaattgc aaacttaatc
     8281 gaaagaaaaa agccagaggt tgtattagtt ggagctactt ctataggaag agatatagcg
     8341 cctagaattg caggtaaagt tggaacaggt ctaactgctg actgtacaaa acttgaaata
     8401 gattcaacag ataataagtt attacaaaca agaccagcat ttggtggaaa tttaatggcc
     8461 actattgttt gtcctaaaaa tagaccacag atgtcaacag ttcgcccagg agttatggca
     8521 aaagctgtaa gaaatgaatc agagactgga attttagaag ttgttactcc agaacttact
     8581 gaaaaaatga ttagaactag attagtagag atacttccac aagaaaagaa atctgtaaat
     8641 ctaactgatg ctagaataat agtatcagga ggaagaggat taaaaagagc agaaggattt
     8701 gagcttataa aagaattggc tgataaattg ggggcagaga ttggtgcttc aagagcagca
     8761 gtagattcag ggtggataga acattctcat caagtgggac aaacaggaac tactgttaga
     8821 ccagaactat acattgcatg tggaatatct ggagcaatac aacatttagc aggtatgagt
     8881 gattctaaat atatagttgc tataaataaa gatgcaaaag cccctatttt tagtatgtgt
     8941 gactatggaa tagtaggaga tttatatgaa attatacctg aaatgataga atcattaaat
     9001 aggtaacttc tagaaaacat tgaatatgta gaatgataag tcttataata ttgtattgta
     9061 agacttatta tttttgctga aggttatagt ggggagatga aacaatgaaa gaacagtggt
     9121 ataaggatat atttgcaaga gtattatcta tgactgatga tggttttatt gttgtaaata
     9181 caagtggagt aataatagat attaatgata aatattgtga ctttttagga aaagaaagaa
     9241 aagatataat aggacacagt atacaaagta ttataccaaa tacaaaaatg ttagacgtta
     9301 tgagagataa gtactgtgaa gaaggagcta tacatcatta ttcaggtgga aatacaaaag
     9361 aaaaaagtgt gatagtaagt aggtcttatg tggaaaatga caatggagaa gtagttgcag
     9421 gagtagctca agttaaattt agattgcaat cctttgatgt ggctaaaaag ttaatgtcag
     9481 aatatatgga gcttcaatat tataaagaac agtttaagga taattgtggt tttgacaagc
     9541 tcataggtga aaataaagat tttatagaac ttaagaaaac aggtgtgaaa gcttctaaaa
     9601 caaactttcc agtactttta acaggtgaaa caggcacagg gaaagaagta tttgctagag
     9661 caattcacaa taatagcagt aggtcagaca aacctatggt tagtataaac tgtgctgcaa
     9721 taccagaaga attattagaa tcagagttat ttggttatga tgaaggagct tttacaggag
     9781 caaaaaaagg tggtaaaaaa ggcaagtttt tagttgcgaa taatggtaca atctttttag
     9841 atgaaatagg tgacatgcct ttgactatgc aagcaaaact tttaagagtt cttcaggaaa
     9901 gtgagataga accagtagga ggtcttaaaa ccataaagat agatgtaaga gtaatctctg
     9961 ctacaagaaa gaatctaagt aaaatggttg aagaaggatt atttagagaa gacctatact
    10021 atagattaaa tgtaataaac attcatatga tggaattgaa ggacaggcaa gatgatattt
    10081 tattgttagc aaattatata ttaaacaaat taaatgttga atacaaggaa ttgaaggttc
    10141 taagtgataa ggttaaaaat tgttttatta attatacttg gccaggaaat ataagagaac
    10201 tacaaaatgt aataaaaagt gcctatgctg taagtgatga tatggtcatt atgatgtgtg
    10261 accttccatc taaaatggat aatatcagta gagtagctca atgtaatgtg gatagtaact
    10321 gttcaattca tgaaatggtt gagaattatg aaaagagttt aattattgat gtgttaagga
    10381 agtacaattg gaaatgttct aaggcagcag aggttatggg aatacacaag agtcttttat
    10441 ataagaaaat aaaaaagtat gaaattgaat taaataatta agatgacttg aattaaagca
    10501 atgtgcaaaa gaatcaacat tatacaaggt gaactgtata aactgtttta ggtagttaaa
    10561 atgtgcaaaa gtagttgtga agtactagtg tcaatcatta gtactttttt attgtattta
    10621 taattactat ctatatttca ttatcttttg atgtctaaga ttattatgtt taatttagga
    10681 cagaataaaa ccctcttagt tttggacagt tttcatattt taactgtcta ctaagaggga
    10741 tagtttaggt caaatttaga gtgaatttta tctagcattt ataaaaatat tttgaataac
    10801 ttttttgtta tttaatcata attttctact tctttatcaa gttcctttcc acaagaatca
    10861 cacttatata tagcattata tccgtaatca tattcatcaa ctattttctt ctgtctaaat
    10921 acaccaccac actcacatag gaaaccaatt tttataagtg taacgataaa ttgtgacttt
    10981 ataaatttta tatcattaca tttgtcgtta gtgtaaaatg tgtaaatata agagttatcg
    11041 cactcatctc ttaattcaaa tttttcaata tatgtgttat tttttaattc ctcaaattct
    11101 tctttagtca aatatgattt ttgttgcaat tcatgtaaac gtttgatttg gtccattatt
    11161 tatatcctcc caataatttt agtattttaa gcttagataa ggaatctaag ttttattaaa
    11221 tttatcctaa ggaattattt aaatcatatt tagttttatt aattgcaaca tatatatatt
    11281 atacaaaaat aaaactaatt gtaaaaataa tatcctgtga aaacctaaac tactaacccc
    11341 ttactaaccc gaaaactaaa aaaagaaaat atatccaaat ataagtgata tataaataaa
    11401 gatgttttat ataaatttaa ctttaaatat tctgacaata atatatataa atatattata
    11461 gttaaaatat accacatatt ttgggtattg tatactagaa ttagattaat taatttattt
    11521 tttaacatac aaatgattct gtgaaaaaaa acaagactca aaaattttta tattctttga
    11581 tagaataaaa ttgagagtaa atttattgaa ttttataaaa tttataaggg gtgaatgtaa
    11641 gatatgcatg aagataaaaa tgacatattt gaatcaatag atactgagga ttttgataat
    11701 aaaacagaag aagaatgttt agagctagta agaaaaaatg gattgaattt aatatacata
    11761 aaaaatcaaa cagaagagat atgcctagag gctgttagac aaaattgcaa tgcaataaaa
    11821 tgtgttcaga atcagacaga aaaaatatgt atagaggcgg ttaaacaaga ttggagaatg
    11881 ttagaatttg tggaagaacc aacagaagaa atttgtatgt tggctattaa ccaagatgga
    11941 acagccttaa aatacataga aaatcagaca gaagagttgt gcctaagagc tgttgaaaaa
    12001 aatggtactg ctttggagta tgtaaaagaa caaacagaag aaatatgtat agaagcagtt
    12061 agaaatagtg aatttggatt ggcaagagtg aaaaatcaaa cagaaaaaat atgtatggaa
    12121 gcagttaaac attgtagcta taatctaaaa tgggtagaaa atcaaacaga agaaatatgt
    12181 atggaagcag ttaggcaaaa tgggcttgat ttgaaatttg taaaaaatca aactgaaaca
    12241 atctgtttaa gagcagtaag acaaaatgga ttggcattag aatttgtaaa agaacagact
    12301 gttggtatat gtttaaaggc agtaagacaa tatggaatgg cattgaaatt tgtaaaagaa
    12361 caaacggaag aaatttgtac agaagctata aaacaagata aaagggcact tagttttgta
    12421 aaaggagaca aggaaaaata caaggctcta tgtgataata atgaaccatt tgcaaagcga
    12481 tatgttagaa atgtaataga aaaagaaaat agagcattga ttaaaaaagg tgaagaaaat
    12541 gcaaaaatca aaattgcagg aaaactttat gatagaggta tgtcttttga agatatatca
    12601 gatatagttg agattgaaat aagcaagtta aaagtgagct taggtatttt ttaagctaat
    12661 agatgtaggt gttttttatg ggaaatagtt aataaaaaaa taatcgacga gttctttgta
    12721 gtacagcaaa attagtgcta taatttgata tataataaat tatttttatt agataagggg
    12781 gaattaaagt tgaagattac aatattatac caaacaagaa gtggaaacac agaaagggta
    12841 gctaagctga tagaagatgg agctaaaaag gtagatggag tagatgttaa acttatgaga
    12901 cttgataata ttgatttaga ttatttaaat gaaagtaaag cagtaatatt tggaacacct
    12961 acttacttag caaatacaac ttgggaagta aaaaaatggt ttgatgaaga ttcaaaaaaa
    13021 gtcaatttag ctggaaaatt aggagctgtt tttgctacat gtgattatat atgtggaggc
    13081 ccagatgttg ctatacttac tgtagtagga catttgatgg ttaaaggaat gcttgtgtat
    13141 tctggaggtg gttctttagg aaaaccattt atacatttag gacatgtaca ttcaattgaa
    13201 ggtccagaac tacaagatga gaaggctata atatttggtg aaagaattgc tactaaggca
    13261 aaagagctat ttaattaatt tctatatgca taatgatgat taaaatattt aaaatcaaac
    13321 gtcaatagtg agggaagttt atggtatggc atatagaata tgaggttttt tctgcaatta
    13381 tagttatttt tttaatggta tactttttta gaagcaaatt tatacctact ttacaaaaca
    13441 aaatatattg tgcactttta atattctcat ttttatttat aatttcaaat attttaggtt
    13501 cattttgtct aaataatata gagaaaattc caatttttat tactttttta ttgaatcaga
    13561 tatactcatt gttattgcca ataccagcag cacttgtatc attttatgtt atggctatta
    13621 tttatcaaga tataaggtat atgaaaaaaa gattattatt tttatctata ccacttttaa
    13681 tctgtataat cttatctatt acaaatcctt ttacgcatat tctatttgga ttgtcaaaag
    13741 aaacaggtta tatacgaggt tgtggatatg ttgctacttt ttcatccttt ttcttttatg
    13801 caatatattc agcactttta tcttttaaat atagaaaagc aatgcatgaa tcaaaaatat
    13861 gggctattag aggttttctt atagtttcaa ttgtggcaat tatcttacag gcaatttttt
    13921 tacagtatct tcttacagga actgcatgtg catgtagttt acttttgaca tatctttcta
    13981 tgcagaatcg aggtcttatt attgatgacc ttactggtgt actcaataga caatctttta
    14041 ttcaagagct agatttgaat atcaatactg aacaatcggg atttattatt acaattgcac
    14101 ttgatgactt taagttcatg aatgaaactt ttggaacaaa aaatggagat attgcattaa
    14161 aagaagttgg aaaatatttg gttcatatac atgatgtaaa ccatgtatat agaatagggg
    14221 gagatatatt ttctattgtt gtcaatagtc aattaggtat aaaaccagag gacctcatca
    14281 aaaagataga agaaagattt aaaaaaactt ggctagtaga agagataagt tttaatttag
    14341 caactagcat tgctgttgtt tattacccag aaaatgcaga aacgacagaa gatgtaattg
    14401 ttgctataga tttctctata catgaagcaa aacgttctga gtcaaggcga gttgtttatg
    14461 cagatgcgtc tataagtgaa aagataaaga gaaaacatgt gattaaaaat tgtttagaag
    14521 aagcattaaa aaatgatgga tttgaagtgt attatcaacc tatattttct aatagcagag
    14581 gtaggtttac ttcagcagag gcattactta ggttggaaca taaagagtta ggttttatac
    14641 cacctgatga atttattaca attgcagaac aaacagggtt gattaattta attggattag
    14701 tagtctttga gaaggtatgt agatttatag ccagtgatga gtttgaaggt ttaaaattgg
    14761 ataatatagc tgtgaatctc tctgtagtac aatgtatgca aaaaaactta gctgatgact
    14821 tgctttatct tatggataaa tataatatat caccaactaa atttaaactt gaaattactg
    14881 agacagtagc tatgggatct tttaatataa ttaaagaaac aatggaaaga ttaattgatt
    14941 taggtgttaa gtttgcactt gatgattttg gaattggtta ttctggagtt tctaatatgc
    15001 tttctttacc gttttcagtg attaagttag ataagagctt gatttggtct atgaatgaag
    15061 acagtagaca taagcttaca gttgaaacga ttatagctct tataaataaa ttgaatatga
    15121 aggctgtagc agagggagtt gaaaccataa aatgtgctga agatttaata gctatggagt
    15181 gtgaatactt gcaagggtat tatttttcta aaccgattcc agaagacgct tttaaaaatt
    15241 tgttgaaaga aaatgctttt acaggttaat accctttatt tttttaatta caattttgtt
    15301 cattgacact gccagttata tattataaaa ttaacacaat aataaatatt aaacttatta
    15361 tacatattat taatattaat tcatgaagta aagatttatt atatattttt ataacttaga
    15421 atatatattt tatttaatta tcctaaagtg agaaaggaag atgtgacatg aataaaaaga
    15481 aaatattagt gccaattgat gggacagaaa gaagtatgca ttctttggaa tttataaaag
    15541 gaatattcaa aaaagatgaa gttgaagtag aaataatgaa tgtaaaagaa ctagtattta
    15601 ttgatggcat atccctggct gaagagataa aaaattctga aaacttagga agaagaatct
    15661 tggataaagc agcagagata atgggagagt atgatgttaa ggtacatttt acttttggat
    15721 atccaggaga cgagataatt agaaaagcaa aagaagataa tgtagatttt atagttatga
    15781 caaaatctac taaaaagggt cttacaagaa tgataggttc agtaacagca agtgtagtta
    15841 aacaagcaaa atgtatagtg atgattgtac cggagtagaa ttagaaaggt atttgaaata
    15901 tgaataaaaa gatagtagca gtttgtgaaa gtgaaagaag tttaaattct cttaagatgg
    15961 ctgcaaatga tttagggttt gacttagttt atgaaattca agaaaatgat aatataaaaa
    16021 atgaactttc tatttatgat atagaagacg catctatagt gttatttgtt acaagtgatt
    16081 ctatagaaag tatagagaag attgatagat ttatagatag agaattttat gaagttgatc
    16141 caaagtatat aatagaggat gcaaagtcta tagtaagtga aattttaata gacttaaatt
    16201 aattaggggt gtctcaaaat gaacttttta gttcattttg agacactttt tttctatgaa
    16261 atcaaatata tttttatcat ttcaacaata aaaatgtgat tatctctatt ttgacacaac
    16321 cctttgagat aattcatttt tgtatgacag ttgtgcttag gaaaaataga agtttaatca
    16381 tcatgctgaa atagctgaat gacatcttct aaatgattgt acattgatgt ataacctaag
    16441 ttagagtctt tactatcaaa ggctattatt atgttattat gatggctaat catatctttt
    16501 agagaatttc tatatttaaa accaaagtat tgattagaag aaattgtttt aggtattaac
    16561 tcataatacc ttataaaaat tgggttatga gaggctagaa ttatttcttt gtgaaattgt
    16621 aagtcagcat cataacattc ttctatcaaa ccttgggaga ccttatccaa ttcttctatt
    16681 gattttaggt aagtatattt tattttttga atttctttag atgtagcatt taaaattgct
    16741 agtctagtag cttcactttc gataagttgc cttgccctta aagattctac ataaaattct
    16801 tggatgttat gatttttata gatttcacca tcatcaaata ggtcaatttt cttaaatcct
    16861 tcaactatag gacttgagta aggcacagcc ctaagtattc cttccttaca gagtatattt
    16921 atggcatttc ttacaggtat tctacttatg ttaaataggt cagcaatctt tctctcactt
    16981 ggtaatttac tattaatttt aaaagttcca tttagaatgt ttaatttaat ataatcaata
    17041 attttttcag tggcatttaa gcttttcata aaaactccta aatatataat ttttttatga
    17101 ttgtattata tcatagttaa gagttaaatc taatttttta taatatgtat aagataagat
    17161 agaagccact atagctataa tagctaagac aaagtatcca tatcgtattg aagtatattc
    17221 cgatatttta ccaactatca ttggaaatac agacattcct atagcataaa ttgcttgaaa
    17281 aaagcccatt gcagtagacc tctgatgttt tggtacattt ttcatagact cgctagtaag
    17341 gtaagaaaga agtattcctg tagatatacc tggtagcact tgtagtacaa atattatatt
    17401 gatatttttt attgttggaa ctaaaaagca gtaggctgaa actaaaataa aaactaaagg
    17461 aatccaaaat tttggttcat acttagtaca aaatttagta gaagcaaagc cagaagaaat
    17521 aacagcacac accatgtaaa atatagaaga tagacctata agggtagaac tagaacctaa
    17581 gtttttaaga atttgagtgg taaatgacat tgttgtagac atttgaatac cttgttgaat
    17641 taatgccata aaggaaaaaa atataagtct tttatcctta catatagata agtatttttt
    17701 aattgatatt tttttaggca ttttttttga attatgattt tttatattta aagctagaat
    17761 cgttccaata actgaagaca atacactcat aatacataag gctgtcattc ctaatttttc
    17821 ataaaataaa gtactcataa caaatccaat caacattcct agattgttta ccagtattat
    17881 cgtacttgta gctttttgtt gatgttcttt agaaaaaaaa ttggtatagt gaaccatgta
    17941 agaaatccaa gttgatgatg atattccaga taaaatgtta gctagaagaa aaccattgcc
    18001 ttcaggaaaa aatactctta taagagatgc caaaccagca aagaaaattc cagatagtat
    18061 aaatatttta tgtttattta ttgaatcagc aaatactcca acaggaagtc ttagtaaaca
    18121 ttgtgagatt ccatatgcac ctattattat accaatcata tttgtagata cattgcatgc
    18181 acttaaatat ggactttgga aaggtatata tacatattgc gaaaaccaga aaaacgtaac
    18241 aatagaaagt aaaagtaatt tttgattaga tgtttttttc atattatcct ccatatggta
    18301 tatttataat gcaaataata taccatttag attttttaat caagtacttt ctctattaaa
    18361 aaaaatttaa atatataatt attatttaca aaaaaattat aatttatagt tgaattaaga
    18421 aaacgcttat gatactataa agttaacaaa tttgaatata taacagtaga cgtgttactg
    18481 gtaatgcagg caagatcgaa tcaattttta tagattatct atagagtact atgggtataa
    18541 tctatttatt tggttcgatt tttttgttta aatggccata aaacaaaaag ccgataattg
    18601 aaaatggagg attttttatg gattataaaa atatagctca agaaattcta ctaaatgtag
    18661 gaggaaaaga gaatgtaaat gaagtgactc attgtatgac aagacttaga tttaaagtca
    18721 aaagtgcttc taaagtgaat aaggataaat tatctaaaat tgaaggagta ataactgttg
    18781 ttgagagtat gggtcaaata caagttgtaa ttggcaataa agtaaaaaaa gtatatgacg
    18841 aggttataaa aattgtacct caatcaaatg aagtaggtaa aaagaatgaa agtgaagaaa
    18901 agcaaggtat aattaattct atattatcag cagtagctgg tatatttaca cctacaatac
    18961 ctgctatagc aggtgttggt atgattaaag gtatcttatc agttcttgct atgtattata
    19021 tgaataaaaa tggagtagat ataaaagaaa ctcaaagcta tattatttta aatgctatgg
    19081 cagattctat attctatttt atgcctatta tactaggata tactgcggct aaagtattta
    19141 atgcaaataa aataatatct atggtattag gagctacact ttgttatcca acttttactg
    19201 ctttgatggc aggtgaagaa gctgttagat ttttaggctt ggcagtaaca aaagctacgt
    19261 atacatcatc tgttattcca ataattattg ccatatgggc tctttcatat gttgaaaaag
    19321 tattagaaaa gtatatacct gaggtaatca aaataataat ggttccaact ttatcattgg
    19381 ttataatgtt acctgcaaca ttatttttat ttggaccaat cggaatttat ataggaaata
    19441 tcataaactt ttcttataag tatatttatg aattaagccc agcattgtgt ggagcatttg
    19501 taggtggatt atggtgtgtg ttggttatat ttggtgcaca tagagcgcta ttaccaatag
    19561 gtataagtga tgttgctcaa acaggaagac aaaatttatt ggcatttgca ggagctgcaa
    19621 acttttcaca agcaggagct gctctaggag tattctttaa aactaaaaat caaggactaa
    19681 aaacaatttc tatgtctgca actataacag ctttatttgg tataactgaa cctgcaatat
    19741 acggtgccaa tcttagatta aaaaaaccta tggtatgtgc tgtaatatgt ggtgcagttg
    19801 gaggtgccat aatgggaatg ggtggagctt atggtaatgc atttgcaaac caaggtgttt
    19861 taactatacc cgtatatgct gaagcaggtg cattaggttt cttaagttat ctaggtggct
    19921 gtgctatagc attttttggt tcagctattt caacttatct agttggattt gaagatttgg
    19981 aagagtcttc taataaggaa aattcattta taaaagtaga aacgaaagat ggtgctattg
    20041 atataacttc acctgtggag ggagaatgta tagaattatc agaggtaaag gatgacgtat
    20101 ttgcatctaa agctatggga gaaggtatag ccatattacc tacaaaagga gtaataacag
    20161 ctcctactga ttgtgaagta gcttctttat ttccaacact acatgcaata ggattaaaac
    20221 tagataatgg agcagaaatg ttaatacatg ttggaattaa tacagtagag ttaaatggta
    20281 aatactttac aaaacatgtc aatcaaggag atttagtgaa gaaaggtgat aaacttatat
    20341 cttttgatat agataaaata aaaaaggctg gttatgatgt aactacgcca gttatagtaa
    20401 acaatacatt tgactttgga caagtagttt cttgtaaatc tagttatgtt tcaaccaatg
    20461 ataatataat ttcattagta ttagctggta attaagttaa tttgactaga gataagtaaa
    20521 ttaacaatta aatacatcta aaagtaagtt tattgtaatg aattattaaa aaaataatta
    20581 atatagagta aatttatttt aatgaagtaa caaagaaaat agttaatata aagtgaactt
    20641 attgtaatga attattaaag aaaataatta atatagatta aacttatttt aatgaagtac
    20701 aaagaaaata atcaatataa aaaggagaaa tatttatgaa tacaggattt ccaaaagatt
    20761 ttttatgggg agcatcatct tctgcatttc aagtggaagg agcttgggat aaagataata
    20821 aaggaaaaac cgtagctgac tataattctt ttaaaaagtc tcatttacag gcagatacta
    20881 aagtggcatc agacttttac aataattatg aagaagatat tgaattaatg aaagaattag
    20941 ggatgaaaac atatcgtttt tcaatttcat gggcaagaat aataccagat ggtgaaggag
    21001 agataaacca gaaaggtata gatttttata ataagataat agacaaacta atagagtgtg
    21061 atattgaacc attcgtaact ttataccatt ttgaccttcc atttacatta gtagagaaat
    21121 ataatggctg ggaaagtaga gaaactgtct atgcttttga aagatttgct aaaatatgct
    21181 ttaagcattt tggagataga gtaaagtatt ggcaacctca taatgaacag aatttaattg
    21241 ttagagtgga agagagaatt aatatatatg atgaaacaga ttcatggaaa atagataaaa
    21301 tgcgtgctca aatggactat aatctttgtt tagcacatgc attagcagtc aatgcatgcc
    21361 atgagatgat aaaagaaagt aaaattggag cagcagtatc atcatctgta acatatcctc
    21421 ttacttccaa gccagaagat gtatatgcag ctagaatgaa tgataatttt aaagtgtact
    21481 atatgcttga tatgcatcac tatggagaat atccagggta ttatatgagg tatcttgaaa
    21541 aaagaaatat agtccctcat atggaagatg gggataaaga agtacttaaa aaggctaaaa
    21601 tggattttat agctgtaaat tattatagaa caaattgtgc aggagccttc ccagaggatt
    21661 cacaacatcc atttgggtta agagaaggaa ctgttgattt tagtatgtat ggattattta
    21721 aaatgtcaat gaatcctaac ctagaagctt ctgagtatgg agcagcaata gacccatcag
    21781 gtcttagagt tgccttaaat gaatattggc aaagatatca cttacctgtt ataataacag
    21841 aaaatggtct tggagctaaa gacatattag aagatggaaa aattcatgat gactatcgta
    21901 tagattattt aaggagtcat ataaatgcat gtagactagc tattgaagat ggtgtagaga
    21961 tgattggata ttgtccatgg tcatttacag accttttaag ctcttctcaa ggttttaata
    22021 agcgttatgg acttgtttat ataaatagaa ctgaccatga agttttagat ttgaaaagaa
    22081 tcaagaaaga tagtttttac tggtacaaag aagttataga gaacaatgga atagtaaaat
    22141 aaacataaat atatattgag ggttttagta tgtacattac aaaggtatta aataattcgt
    22201 tgctacttgc aagggatgat aatgatgaag aaataatatt gatgggaaaa gggattggcc
    22261 ataattataa ggctgggtct gagttaaata aaaaagatat agaaaagatt tttgttcttc
    22321 atgatgaaaa tataaaaaag agtattatac aactggcaac agaaatagat gcagaatatt
    22381 ttggtatagc tcaaatgata attgcatatg gaatagaaaa atataactta aaacttatga
    22441 accatatata tttggcctta actgaccaca ttgcctttgc agtgagacgc tttaaagaag
    22501 gtataatgat tgaaaatcac tatttgtttg aaatcaagga gtttaatcca aaagagtatg
    22561 atataggaaa atatgcaatt actgttttta aggaggttct tggtttagaa ctaccagaag
    22621 aagagattgg taatatagca ggccatttta taaatgcaca acaagataat ccttatagtg
    22681 atagaaataa gagaagtgct aaaattgtaa atgctatttt gcaaatagtt cattatcact
    22741 tttctattgt atataataaa gagtcatttt actataggag atttgtaatg catctaaagg
    22801 ccttttcaca aagatttctg gctaacgaac cgtcaaaaga aaaaatagat tttatatatg
    22861 agcaagttca aaaaaattgt aaggaagagt atgaatgtgt gaaaaaggta ggagcttata
    22921 tgtataagga atactccaga gaattaccaa gacaagagga gttatatttg atgattcata
    22981 ttcataagat tttaggagaa ttatgcgaaa atagttagaa tatcttaata tgctagtaat
    23041 attataaaaa attaaaaatt aattacactt taaatatgaa taattgcatt acttaagggg
    23101 gttgtcttaa aattgatttt tttaattaaa ttttaagttc aactcctttt taaaccttta
    23161 tatcattaca actttaatgt ttatacgcct gtacttcaga tgtgtatcta ttttttacat
    23221 ttctcaacat gaaaatttat cattttttgt agtgtgttaa ttgaaataaa cctttgagtt
    23281 aaccctaaat ttattctgga cctacaggaa tagttccaaa gatactttaa aatattttct
    23341 ggaatgattg cttatttcat atatatttta ttgtgatgtt ttgatttaat aaattcttca
    23401 actccgtata tagtaaatgt attatcatgt ttgttttatt taagcacact tataatatgc
    23461 ttgatttttt caaaatgatg aaatatacta atgggtgttg gatgtataag aatcacatta
    23521 tacttgaaaa agtaagtgag aaatgaggta taattcctca tatgataaac tactattgta
    23581 ttgtggaaca tattctcagt gatgtcaaga tattacaata tgactaaatt cctaatgata
    23641 aaaaagtcta cgaatgagtg tagaaagggg aactaagatg aataaagttt taattataga
    23701 tgatgataaa gatctttgta ttttgttaaa aaagagtatc tcaattgaac atattgaagc
    23761 aaactattgc cattctggga gagatggatt ggttgaactt tcaaaacaag attatcagtt
    23821 agtagttcta gacgtgatga tgccaggtat ggatggtttt gaaactatgg aacaaatacg
    23881 aaaatatagc agtattccca tcttaatgct gacatcgaaa aatgataatt acagcaaagt
    23941 tcatggattg cgagctggtg cggatgacta tctaaccaaa ccatttgaaa ttgcagaatt
    24001 tactgctcgt gtactgtctt taatccgacg ttacacccgt tttaatccaa agaatgagga
    24061 gtcacagcaa ctttctttca aaggtctgtt gattgacctt gaccagcata cagtgactac
    24121 caatggcgaa actatgttgc ttccacccaa ggaatttggt gtgcttcttt actgtgcaag
    24181 gcatcagggt aagattttga caaaacagca gatttatgaa gctgtgtggg gtgagccata
    24241 tgtatatgat gacagcaata tcatggctat tatcagtcgc ctaagaaaaa aaattgaact
    24301 agacccatca tctcccatct atttgcaaac gataaaagga atcgggtatc aattcaacac
    24361 ggaggtatga aaatggatga acaaacgata attctttttt tgtttttgct gtcgattggt
    24421 ctacttttcc tttccatatt tgctttttcc aagcttggtc atattcacaa acgtctaaaa
    24481 gatatagaag aaatacttgc tgatgtacaa attggaaacg aaaatcgaaa aatactaatt
    24541 aagccatgtg atgttatggc tccattggtt tatcagctta atgagattgt ctacgattat
    24601 gaaaataagt tactttctct gaaaaagtcg gataaagcta gcaaacaact tatgacaagt
    24661 ttatctcatg atgttcgtac accattaacg actctgattg gttatctgga tgctgtacac
    24721 agtggtatag ttatcggaca agaacgggag gaatacattg aaatcgctcg gcgtagagcc
    24781 tacgacctta aggattatat cgatgttctt tttgactggt ttcgattgaa ctctgatgaa
    24841 tttacgttgt caatagaatc tgtggaaatt gcagagttgt cacgtaatat tcttaaagat
    24901 tggattccta tcttccatga aaagaaattg gattttgaaa ttgatattcc agaaaatcga
    24961 ctaatggtaa accttgattc agatggatat tctcgtgtag tcaataatct agttcaaaac
    25021 gttcttgccc atagcaaggc tcgccgaatc aaaattacca tgagtgagga ttctaagatg
    25081 gtattacttc gtgttgagga taatggtgtt gggattgcca aagaaaattt acagcatatt
    25141 tttgagcgac tgtacaagtg tgataaagga cgttcaaaaa aaggaagtgg tcttggttta
    25201 tccattgtca gtcagatggt ggagcgaatg ggcggtaaga tttctgtaga aagtgaaatt
    25261 ggaaagtata ccgtatttat agtgagctta ccattaatat aaaaattgtt tttcccccat
    25321 gtgatatata tacacagggg ggatttttta taagctataa agttgcaagg ttaatacaag
    25381 gttgttgcaa ggttaatgta agattgaggt ggtatgataa atttaataaa caaaggagga
    25441 ttttactatt tatgaataat ttaattattg aaacaaaaaa tctcactaaa caatatggtg
    25501 ctcaaaagag cgtttctaat ttgaatattc atgttcagaa aggtcgtatt tatggattgc
    25561 tgggaagaaa tggttcgggt aagacgacaa ctatgaaaat gttattgggt ctaacaaaac
    25621 caactactgg agaaattaaa atatttggtc aggatattca taataacgag aaaaagcttc
    25681 ttcctagaat tggatgtttg attgaatcac caggttttta tcctaatctg acaggaacag
    25741 aaaatctcaa aatctttgcg aggcttagag gaataccaaa aaaaaatgca gtagaatcag
    25801 cactggatgt agtcggtttg ccaaacaatg acaaaaagtt attttctcaa tactctcttg
    25861 gtatgaaaca gaggttggca attgctcttg ccattatgca tgacccacaa ttgttgattt
    25921 tggacgagcc tatcaatgga cttgatccta ttggtattgc agaagtaaga aagtttattc
    25981 gtaatctctg cacagaacga ggtaaaacta ttttgatttc cagtcacatt ctttctgaaa
    26041 ttgctttgtt ggcagatgac attggcatta ttgaccgtgg cattttgttg gaggaagaaa
    26101 gccttgcaca actggaagaa aaaaacagta aatacataca ttttgctgta tctgatgcag
    26161 cacaagcagc aagacttctg gaaatccagc ttaacctgaa aaatttcaaa gtggcagaca
    26221 atcacaatat tatgctttat gatacagatg tctctgttgc agaaataaat cgtgctttca
    26281 ttcaaaacaa tattgatgta tcagaatctc atttgtgcga agatactctt gaagattact
    26341 tcaagaaagt aacagggggt gaaggaattg cttgatataa tttcatgcga attttcaaaa
    26401 ctcaagcggt taaaatttat actgatttct attttaggtg cttgtttatt tccaattccc
    26461 gcaacaattt tgattgcaaa ggacaacttg ccttttgagc aattgttcaa attagttgtc
    26521 aattttggat attttttgct attaccaatt gtattgagta ttgttgctag ccaactcttt
    26581 ttcatagaaa gagataacga tgtattaaaa aatctagcaa cagtgccagt gccaaaagga
    26641 aaactggctc tggcaaaatt agctgtgttg ctgtttatat cattgttcta ctccgttgca
    26701 gggttaggag ctactatcat aggaggtttc attgttggaa tagtagaggg ggttgctgta
    26761 aaattaggaa tgagtattgt ttttggaatt atgttttttg ttactgcact tcctgttgtg
    26821 gtactcattg tatatttcaa tcgcagctat attttttcta ttatactttc ttttgtatat
    26881 gctattttca attttagtat atccttaaat attatcaact ttgaaccaaa taacccactg
    26941 ttaagcgtat tacctgctcc tgttataatg cgttggtgga tggcattttg gggagatccc
    27001 actggcgaat acacggcact tagacagcca tatttactct caactcctgc ttgtgtaggt
    27061 attttatgtt tgataacaat tattgcagtc ctgttgattt gtataatcta caagaagcag
    27121 gagaattagg aggtatctat gcttgatata attactacag aatttcaaaa aataaaaaga
    27181 tataatattc tatggattgg tgttgttgct gttctgtttt cagcactatt ggcggttttt
    27241 cagattgtaa gttcacacgg cagtgaccct ctaacgtatg agagctttgc aaatggagtt
    27301 atatggaata atttcagtct tagttttcca tttgtgatta cgctcattgg aggatatctt
    27361 attaaccgtg aatatacaga ccaaacctta aaaaacatcc taactattcc tatttcatta
    27421 cgcaaattgc tagtgggaaa attaattgcg gttggaggaa ttactgtatt gttaagtatt
    27481 ttcagcttct tatgcacatt tattttaggg ttactatttt gcaatatcaa tatatctgtt
    27541 atactcgttg caaaatcctt agttcagatt ttgtctgtta atctgtattg ttatgttgct
    27601 gttttgccga ttattgctta ttttggtcgt aaacaaaatt cctttttgac aggcgtaggg
    27661 attgcatttg tatatggctt ctgtggtgta tttgttgctg gaagaaatct aacagacttt
    27721 tatccaatca cagcaggtct tggtctagct ggatacaaca atgatgatgg tagtgtttat
    27781 cagccactta taggtgttat gactttggtt gtaattctaa tcctgacagc tattctactt
    27841 acatttacac ctaattacga taaagttatg accatatcta agaaaaaagg gataaagcat
    27901 aaaaagtatt ctcataaata acataaatat tgtttctatt atttaagttg tcaggcagtg
    27961 aaaagaaaaa atctgtcgta cagtggctat atgctcatgt atttaagaag ctacagtttt
    28021 gatttttcat aggtgtggct tctttttgta tttattaaat caacaactat cataatattt
    28081 gcaataggta tagtaacaag agataatact gtcatatgtg ttttatatat tacattaagt
    28141 atattctaaa atatatgttt taaataatat atttgaaaat aagaatactt gaataaaaaa
    28201 aataatatta tatatagttg acaaaaagtg gaaattaata tactatataa atatagaaat
    28261 agtaatcatt actaatatta aaaaaggagg aattaattat gaacttaaaa ggaactaaaa
    28321 cagaaaagaa tttaatggca ggatttgcag gagagtcaga agcaagaaat aaatatacat
    28381 actacgcttc aaaagctaga aaagaaggtt ataatcaaat agctgctata tttgaagaaa
    28441 ctgctaacaa tgaaaaggaa catgctaaat tatggtttaa acttttacat gatggtatgc
    28501 cttcaacaga ggaaaattta aaggatgcag cagcaggcga aaactatgag tggactgaca
    28561 tgtatgcgaa atttgctaaa gaagctagag aagaaggatt tgacaagata gcttatttat
    28621 ttgaggctgt tggaaaaata gagaaagagc atgaagaaag atacttaaag cttttagaaa
    28681 acttaaatga aggaaaaata tttaaaagag atgaagaagt tgtttggcaa tgtcaaaact
    28741 gtggtcatgt gtatgtaggt actgaagcac cagaaaaatg tccagtatgt gaccatccaa
    28801 aagcatattt caatataaaa gctgaaaatt attaaaaatt atcattatta aaaaggaatg
    28861 ttaactatga aattttctaa acaacgagaa ctgattttga atgaaatatt aaataatccg
    28921 gttcatccta ctgcggatta cctatatgaa aacttaaaaa aagataatcc aaatctaagc
    28981 ttaggaactg tgtatagaaa tttatctcaa ctaacggaac atggctttat aaaaaaggtt
    29041 agtattccag ggtatccaga tagatttgat ggtagaatag ataatcacta tcatatcata
    29101 tgtgaagtat gtggagaggt atacgattta gaatctgaag ttcttaataa tttaaaagaa
    29161 ttaatatctg aggagacaga cataaaaata acatcttata acataagttt taaagggatt
    29221 tgtaataatt gtaaaagatg tagccaagta ggttagcttt taaattctaa aaataattaa
    29281 tgaggtgagt ttatgtgtag cgaacaaaaa ttttttatat gtgagacttg tggaaatctt
    29341 gtaggaatga tacaaagtgg tggtgtacct attttctgtt gtggaaaacc tatgaaggag
    29401 cttgttccta atacaacaga tgcagcagtt gaaaaacatg tacctgtgat tgaagtggat
    29461 ggaaatagtg ttactgttaa agtaagtagt gcaattcatc caatgactaa agaacatcat
    29521 atagcatggg tgtatcttat gactgaacaa ggtggtcaac gcaaatgtct agcagtagat
    29581 ggagaacctg tagttaaatt tgctctgaat gatgatgata aagtgatttc tgcatatgct
    29641 tattgtaatt tacatggttt atggaaggct gaactgtaat atttaaataa attgagtcaa
    29701 aaaggaggct ataaatgtat tttcatacaa atagcctcct ataaatttat ataaattcga
    29761 gttttaattt tatttaatgt caatataata taaggagaaa ataatatgtc aatttacaaa
    29821 tgtagcgttt gcggatatat ttatgatgaa tctaagaatg ataaaacatg ggatgaatta
    29881 agtgaagact gggaatgccc tgtatgtaca aaaggtcgta gttattttgg aaagataagt
    29941 actgtttact atgaagaaga tgaaaatata gcagaagaca tagttgaaga tgaacctaaa
    30001 ttaaatactg aaaaagatgg agatttaaat tacgtaagta catatttaag aagagatgat
    30061 gaagttgaga aacatatgga tataattcac gaaatggctg ttacaggaaa atctataata
    30121 gaaccgatga gaactaaact accagtaatt tcttgggatg atattttaat tatgggtgct
    30181 caattaaatc cattaccttt aaacgaacat gatgaagtaa atacaacaac aataataggg
    30241 aaaaaagcaa agaagcctat gattatagaa aatccagttt atatttccca catgtcattt
    30301 ggagctttat ctaaagaact aaaaatagct ttagcaaagg gtgccgctaa aagtaagaca
    30361 gctatgtgta gtggagaagg tggtatatta ccagaagaaa aagaagcttc atataagtat
    30421 atttttgaat atgtaccaaa taaatatagt gtaactgaag aaaatcttaa aaattctgat
    30481 gctatagaaa taaaaattgg tcaaggaaca aaacctggta tgggaggtca tcttcctggt
    30541 gaaaaagtaa ctgaagaaat agcaaaggta agaaataagc ctgtaggtaa agatgtaata
    30601 agtccatctt gttttgagga aatacaatcc aaagaagatt taaagaaact tgtagatgaa
    30661 cttagagaag tttcagaagg tcgtccaata ggtgttaaaa tttcagctgg tcatattgaa
    30721 aaagatatgg aatttatagc ttatgctaaa cctgattttg tgactattga tggtcgtggt
    30781 ggagctactg gagcaagccc aaagttgctt aaagatgcaa cttcaatccc aacaatattt
    30841 gcactttatc gtgctagaaa atacatagat acacatggat tagatataga tttggttata
    30901 acaggtgggc ttcgtatatc tactgatttt gccaaggcaa ttgctatggg tgctgatgca
    30961 gttgctattg caagttctgc tcttatggca gcagcatgtc aacaatatcg tatatgtggt
    31021 tctggtaagt gccctgtagg agtagctact caggatgagg aattgagaaa aagattacat
    31081 atagaaaata gtgctaatag agtggcaaac tttttaaatg taagtttaga agaacttaag
    31141 acttttgcta gaatttctgg tcataaagat atacatgatt taagtgttga tgatttatat
    31201 actgttaact ctgaaatatc aaattacaca aacatacaac atgtataagt tgaagtttga
    31261 gactattaga ttgtagatag ttataaaagt aaatgataaa tatattttat aaaaaggagt
    31321 gtcttaaaat gaacttttta gtttataagt cactcttttt tgtatgaagc caaatatatt
    31381 tatcatttta acaatgaaaa agaatcttat atctttactt tgagatagat tccttctatt
    31441 tatgaaaatt aaagatatta aattgtaaaa cattctgtga tgtcgtacta ttaaactaaa
    31501 gatgctgtac tttaaagtaa ataaaaattt aaggagtgat tattatggaa aaattgaaga
    31561 ttcaagttct agttgacaat aatacatata ttgataggta ctttgttgga gaaccagcag
    31621 taagctatta cattgagatt gatggaaata ggattttgtt tgattaaaaa atagctacga
    31681 acact
//
LOCUS       DAFNNB010000028        31404 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0028, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000028 DAFNNB010000000
VERSION     DAFNNB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 31404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0028"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            204..575
                     /locus_tag="L3M60_001139"
     CDS             204..575
                     /locus_tag="L3M60_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN6225827.1"
                     /translation="MKFNFCHNNFNVTNLEKSLDFYKKALGLKEVKRKEAEDGSFILV
                     YLGDGITSHTLELTWLRDWDRPYNLGDNEFHLALEVEEFDEAKKLHKDLDCICFENES
                     MGIYFIADPDNYWIEILPKNH"
     gene            707..1981
                     /locus_tag="L3M60_001140"
     CDS             707..1981
                     /locus_tag="L3M60_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBN6225828.1"
                     /translation="MKKAIAALGIGAVAVSVSSINASALEKGTVTASALNIRSGPSSD
                     CDKVAKLYKGKTVEILEKSNGWYKVRVSSSVVGWGSAKYISTSGSSEGTSNPNNSTSS
                     GTTISGNGKVNVSSRLNVRSGAGTNYSLVGKANNGEVVKLLEQSNGWYKIKLSNGVTG
                     WASSQYISKTSEDVGANNSSNSNSTNNSDKKPSSEESIEGKNGKVTSTVSLNVRSGPG
                     TSYSIIGKLNGGDVVELKAKNNGWYKVKLSNGTTGWVSGSYISETNEGTKENSNSSSN
                     QNSQSNNNSKPSFTGNSDKSTAKGSTIVDFAYTLIGIPYQWGASGPDKFDCSGFTQYV
                     FKHSVGVSIPRVSREQANFGSAISMGNYAPGDLVYFDTDGDGTTNHVGIYVGNSKFIH
                     CSGTQTNPNKVKVDNLTSSYWSKVLLGARRFV"
     gene            2092..3036
                     /locus_tag="L3M60_001141"
     CDS             2092..3036
                     /locus_tag="L3M60_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225829.1"
                     /translation="MNRRKTIYRGFNNNRHKIDLRKYVITIACLCLIGYYSYTKIKDS
                     KILEYVSAKIPFLNNSSDITYKDISDELNSIKNGKKSKSQTNSDDKQETNPEKAVNNT
                     KEPEEVKLATIEGWDMYTIQVAAIDNNDDLKKIQTSLVNNDIPFSVMEKDGVKKIQTY
                     SSFDENDVRKQISSVRKVFPDAFLSHLDAPMLSLEYTSNYAYIDSISKELNKLITNFK
                     EESSFWSNAENNVDMEKYNTILTNRKAISQNISKEAEKIDYSEMRLFKDNLIEYVKNV
                     NEKIDTASKAANEEKYSVSKSLLLSSMQEYSMFINSIK"
     gene            3252..4589
                     /gene="rsxC"
                     /locus_tag="L3M60_001142"
     CDS             3252..4589
                     /gene="rsxC"
                     /locus_tag="L3M60_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBN6225830.1"
                     /translation="MKLLTFKGGIHPPYRKEYSNTKALEKAQAPKIVYIPLQQHIGAP
                     AKTIVEVGDEVKFGQKIGEQQGFVSCNVHSSVSGKVIAIEQHEVPGGSAQCVVIENDF
                     KEELHESVQPKGQLEDLSKEDIVGIIKEAGIVGMGGATFPNHVKVSPPPDSKAEVVIL
                     NGAECEPYLTADHRLMVENPEDVVFGLRALMKVLDVKKGFIGIETNKPDAIEAIQNVA
                     KDYSEIEVVGLQVKYPQGAEKQLIYACTGKEVPSGGLPIAAGAVVDNVATAAQIAKSI
                     KTGMPLVERITTVTGSCIKEPKNLITKVGTLVSEIIEQCGGFKEDKKIGKVIMGGPMM
                     GIAQYTTEIATNKGSSGILCLDEEESRTPDVQNCLRCGRCTDVCPSFLQPLFISAYSL
                     KDDYDTAEYHRAMDCIECGSCSFICPARRPLLQSIRSAKREIGAKRRKQAAQK"
     gene            4612..5589
                     /locus_tag="L3M60_001143"
     CDS             4612..5589
                     /locus_tag="L3M60_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HBN6225831.1"
                     /translation="MENKLIVSSSPHVRSNEDTSYIMKQVIIALLPAAVAGVYFFRLN
                     ALSAMFFCILGTVGTEFLYQKFAKQKSTIGDFSAVVTGLLLAFNVPASLPWWMCLVGG
                     IFAILVVKMVFGGIGCNFVNPALAARAFLLASFPVAMTAWTQPGVNWIGKNLDAVTTA
                     TPLSFLKNGAAGLADLSSNGISLADMMIGNIGGCIGETSAILLLLGGVYLMYKGIINY
                     VIPVFYIATVFILTFLLGGFNITFAIYQLFAGGLMLGGFFMLTDYTTSPMTKKGQIIY
                     AVLAGLITTVIRMYGGYPEGVSYSILLVNCLAPLIDKFVRNRVFGEVAK"
     gene            5589..6158
                     /locus_tag="L3M60_001144"
     CDS             5589..6158
                     /locus_tag="L3M60_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HBN6225832.1"
                     /translation="MNSMVRLGGTLLAISAIAALALGATNQVTAPVIEQRNIQANNEL
                     RKAVLPEAKEFKEMDKSTYKDLGDGLIAEVYEGLDGSEVVGYTLKAKPSGYGGEIEVM
                     VGISSEGQVTGVDIGNMSETAGLGAKAKDDAFKGQYKGKTAEPLEVAKGSTTADNQIL
                     AISGATITSTAVTTGVNAAIDVFNSALNK"
     gene            6171..6764
                     /locus_tag="L3M60_001145"
     CDS             6171..6764
                     /locus_tag="L3M60_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HBN6225833.1"
                     /translation="MNLAKVFKNGLIDENPTFVQVIGMCPTLAVTTSAINGIGMGLST
                     AAVLICANLVISLIRKITPDKIRIPIFIVVIATFVTIVGMLLKAYVPALDKALGIYIP
                     LIVVNCLILARAESFAFKTGAMPSIVDGVGQGLGFTVALTIIGAVRELLGNGSLFGMT
                     LFGASFQPVLIFILPPGAFLTLGFLFAGFNKLRSKKA"
     gene            6779..7354
                     /gene="rsxA"
                     /locus_tag="L3M60_001146"
     CDS             6779..7354
                     /gene="rsxA"
                     /locus_tag="L3M60_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBN6225834.1"
                     /translation="MNLILLFLSIVLVNNVITSQFLGICPFLGVSKKVDTAVGMGVAV
                     TFVLTLASIITYFIQILLVKTGTGFLQTIAFILVIASIVQFVEMVIQKMSPSLYQALG
                     VYLPLITTNCAVLGIALVNVQKSYNLVETIINGFGAGAGFTLAIVIFAGIRERLELAD
                     IPEAFKGFPITLISAGLMSIAFLGFTGLIKL"
     gene            7370..8341
                     /locus_tag="L3M60_001147"
     CDS             7370..8341
                     /locus_tag="L3M60_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HBN6225835.1"
                     /translation="MVILTAVLVLGIMGLIFGIVLDFASKKFAVEVDERVEAILGVLP
                     GANCGGCGFPGCGGLANAIVEGNAPVNGCPVGGADVGAKVGEIMGISAEAGEKQVAKV
                     ICKGTCSSAKDKYEYEGISDCRAANVLNSGAKMCKFGCLGLGTCKDACKFDAISIVDG
                     VAVIDEEKCVNCGKCKEVCPKGIIITKPESQEVVVECNSKEFGKAVKEKCTAGCIGCG
                     MCVKACKFDAIIFEDKIAKIDPNKCVGCMQCVAKCPTKVISGDITKKKKVTIDQELCV
                     GCTVCKKQCKFDAIEGELKEKHKVDADKCVGCHLCMEKCPKKAIKIL"
     gene            8561..9145
                     /gene="maf"
                     /locus_tag="L3M60_001148"
     CDS             8561..9145
                     /gene="maf"
                     /locus_tag="L3M60_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HBN6225836.1"
                     /translation="MNIILASASPRRKEILENTNVRFDIIKNEIDEIILEGETPKHLV
                     MRLAFEKSMSVASEHNEDVVIGADTVVVLDNAILGKPKDESCARDMLKRLSGREHQVI
                     TGISLINLCEDKKVIDYVISNVKFKTLSEQDIEDYLKTNESFDKAGAYGIQGYGALLV
                     EEIRGDYFNIVGLPISRLSDLLKKYFSINLFYGV"
     gene            9190..9849
                     /gene="radC"
                     /locus_tag="L3M60_001149"
     CDS             9190..9849
                     /gene="radC"
                     /locus_tag="L3M60_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBN6225837.1"
                     /translation="MAPEERPREKMLAKGVKSLSNAELLAILLRTGDKNKNAIELANY
                     IINRDIQGIRHLEDMTIEELCNIDGIGLSKATQIKAALELGSRVASFKPIKYKIRNPW
                     DIQRYYMDSLRYLKKEVFKAVLLNTKNEIISDVDVSVGTLSSSLVHPREVFKEAIRRS
                     ASKIIVMHNHPSGSVEPSREDKNITSRLIKCGEIIGIEIIDHIIIGDGLYFSFKENMI
                     I"
     gene            9970..10980
                     /locus_tag="L3M60_001150"
     CDS             9970..10980
                     /locus_tag="L3M60_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBN6225838.1"
                     /translation="MTKDMGIDLGTANTLVYIKGQGIVVREPSVVAIRDDSKEVLAVG
                     EEAKKMIGRTPGNIVAIRPMKDGVIADFDITQSMISYFIQKAADKKGVVSPRIAICVP
                     FGVTEVEKRAIEEAARQAGAKDAFLIEEPMAAAIGAGLKVEEPEGNMVVDIGGGTSEI
                     AVISLGGIVTAKSIRIGGDEFDESIVAYVKKEYNLMIGERTAENVKINIGSTFKDDEE
                     VNMQIRGRDLISGLPKTIEICSTEVREALKEPVSSIVDAIKSTLERTPPELASDIMEN
                     GIMLTGGGALLRGLDKLITQETGMSVQIAETPLDCVALGTGKSVEDQEIFEKVLMMNT
                     KN"
     gene            10990..11883
                     /gene="mreC"
                     /locus_tag="L3M60_001151"
     CDS             10990..11883
                     /gene="mreC"
                     /locus_tag="L3M60_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBN6225839.1"
                     /translation="MALRFDKNKNEKKRINVKVIATGVVAITLIGIVGISIGKFSSGS
                     PVNLGVASDAITSVGKGINDGFSFIKNGFKDIANFKDNSKKVKKLEEENEKLKKDMIS
                     LNAKLDKTESLEELKKTLNFVQEEYKATSISTSVVSKNDGNWYESFVIGAGKNSGVKK
                     ESIVMNGSGLVGIVYEVSNNYSKAISLLDSKASVSFKLAKDANAKGTITQNTTLDNKD
                     SYNSKGYLQGYMFDSSYNVIQGDIITTSGLGFFPDGIPIGEVEKVVDDKDKSLKYVVV
                     KPYVDFKNINDVVVIEPRNIG"
     gene            11898..12386
                     /gene="mreD"
                     /locus_tag="L3M60_001152"
     CDS             11898..12386
                     /gene="mreD"
                     /locus_tag="L3M60_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBN6225840.1"
                     /translation="MKKVLLCLLGIVLMTIENSITNYIDIFGISFNLVLIYVTIISLY
                     LDELEGGIIAAIIGLIKDITVGGIFGANALILFIIAYAIGYMRDQLYKGSYIITFVLI
                     FIGSLIDSIVNIGTSSIIYQSYNISTLFIKGLLVAPIVNSLIGLVIYRLSKRAVLKLK
                     ED"
     gene            12398..15376
                     /locus_tag="L3M60_001153"
     CDS             12398..15376
                     /locus_tag="L3M60_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HBN6225841.1"
                     /translation="MEENKQVDRLKIIKIVMSVIFLAILVKIIYMTTFKYEYYNELAE
                     NKTYKKLAIEAPRGEIKDRYGRLLAGNKNLFTVQVSGNDINKKDANKHSRANEISLKL
                     INLLERNGEEYVDEFPIYVENGKYYYTYDRDIREYKSENGIPNDYNAKESFYYLVDKL
                     ISAGILSQEDKRLDATRLQAKLNENGYYPPILVSKWMFTAERDKRDWLASYKIKETKL
                     SAKEAFEKVRNSDALEIDKNLSDEDARKIMVVRDLIKSKGYSQYNPVTIAKDVGETTI
                     AQIEESAMDLVGVSIAVEPVRYYPNGSLASHMLGYVGKMPSTQIESYLQKGYETGDMV
                     GLAGVEKSNESRLRGTDGYKMVKVDALGRISKEIESKKPKSGDTVYLTLDKDLQEVSD
                     NALKQIIEVASKGGTFKSKFGDKPISAYAGKAQSAALIAIDVKNGEVLASSSYPNYDP
                     NKFAKGISTEDYKALQPKNPNDLLAGSPLLNLVTQGEFQPGSSFKMVTSMAALENGLN
                     PNFTINDPGVIMLGKKSFGDYVWNHGRGNHGMTNLYKAIQESCNIYMATIGTGKTWPD
                     GKSIGIDMNANKILEYAKLFGLDQNTGLQDEVEERAGKVPSTEDKLKSTQALLKSNLE
                     REMANDFVDITREKNPKEYEKRINEIVSWAAEKKTPGRVETMNRLKKMNVKEDRIEDV
                     ADLAVFSYFNFAKWSTADTFNLAIGQGENAYTPAQISRYVAAIANGGNLVELSVVDRA
                     VSSDYSSVKINDQKKVEKIPFKNPDNLKELTKGMKLVARQGTAKSAFADFPIDVAAKT
                     GTAEKSGKIPTDNEYEYLKSHMSSYNVNLNDAIKLADKMKAEKEKELSLAKEKEIKKK
                     LENKDLKDEERKKLEEELEDGVKVRLEDTDKVNSSYLRKAIKELNPKITDDQIDRFKQ
                     DYGSFTWTVAFAPADDPEIAVVCVIPQGDSSVFSLLPTREVIGTYMGLEPTNSKNDNK
                     TNDVNNSSDENINFESQINR"
     gene            15474..16157
                     /locus_tag="L3M60_001154"
     CDS             15474..16157
                     /locus_tag="L3M60_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBN6225842.1"
                     /translation="MSLREICSQELVEFKGNKRGIIVNIKREAPFEEIQEKIINKLEA
                     YVGFFNGAKISKINSDCLTDMEILELKEGITSRFDVEFVEDQKVEENSNFPTKYVNTL
                     RSGENIEFEGDVVILNDMKPGSKVLSKSNTVVMGDINAGAKVVAGGNVFVMGKIEGFV
                     HAGAEGNEFAYVVAGNLNPKILQIADNIAEAPDDEENYESESEISPEIAFVSNGRIVI
                     ESYLSKLDK"
     gene            16181..16978
                     /gene="minD"
                     /locus_tag="L3M60_001155"
     CDS             16181..16978
                     /gene="minD"
                     /locus_tag="L3M60_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBN6225843.1"
                     /translation="MSEVIVITSGKGGVGKTTTAANLGTALSLENKKTVVVDADIGLR
                     NLDVVMGLENRIVYDIVDVVEGTCRLKQALIKDKRFDNLYLLPAAQTRDKNAVSVEQM
                     IDLCEKLKESFEYIIIDCPAGIEQGFKNAVAGADRAIVVTNPEISAVRDADRIIGLLE
                     ANEIKEIRLVINRIRNDMVKRGDMMDKQDIIEILAIDLLGLVPDDESIIISTNKGEPA
                     ILDSKSLAGQAYKNIAKRILNEEVPLLDLEVEDGFFGRLKKMFSMAK"
     gene            16996..17280
                     /gene="minE"
                     /locus_tag="L3M60_001156"
     CDS             16996..17280
                     /gene="minE"
                     /locus_tag="L3M60_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBN6225844.1"
                     /translation="MLDLFRVFSNEAKTSKSVAKERLKLVLVHDRVDCSPQLLEMIKT
                     DILKVIANYAEIEDDGLEIKMSKCRGEHDDKPVSALVANIPLKNIKDRCM"
     gene            17364..18494
                     /gene="rodA"
                     /locus_tag="L3M60_001157"
     CDS             17364..18494
                     /gene="rodA"
                     /locus_tag="L3M60_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HBN6225845.1"
                     /translation="MKKININYKSTIKLIKQLDWKLIVTVLAIFIFGLVILSSATHAN
                     STGSYNQLIKQGLAFVLGIGMIIVILFFDYNFLGRYYKALYIISLVLLAIVLLPGIGS
                     VKGGARSWINLGPLDLQTSEIVKLTFVLSYAKILESKKDKLNTLKEVMPVIVYSLPFI
                     GLLIAQPDLGTGIVFCCMIFAMLFTAGLSSKLIKRGIIILLVSMPLMYLMMADHQKVR
                     IEAFLNPEDVTLKGNYQVMQSLIAIGSGGVTGKGLYNGSQNQENFLPVQDSDFIFAVV
                     GEELGVIGMAVLIILFMIFLLRLLAIARDAKDFYGTLIVVGVMGMFGYQIIQNIGMTV
                     ALIPVTGVTLPFVSYGGSSLLTSLANLGLVLNVCMRRKKINF"
     gene            18535..18948
                     /locus_tag="L3M60_001158"
     CDS             18535..18948
                     /locus_tag="L3M60_001158"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBN6225846.1"
                     /translation="MNIALVAHDQMKNTMVGFCIGYESILKKYGLYATGTTGKRIMDE
                     TELNINRLASGPLGGDQQIGSLIVTQEIDLVIFLRDPLTSQAHETDIQALIRLCDVYH
                     VPIATNLASAEIFIKALDRGELSWREVRKSKSQRV"
     gene            19433..19762
                     /locus_tag="L3M60_001159"
     CDS             19433..19762
                     /locus_tag="L3M60_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225847.1"
                     /translation="MQLNKEVLKGYIDILIVSILEKKDCYGYEIAKQVRERSEFELKE
                     GTMYLALKRMESKNLIKSYYSNEQSSGGRRKYYNLTNEGKDFLEIKKQEWRFIKKVMN
                     QFLGEVD"
     gene            19766..20557
                     /locus_tag="L3M60_001160"
     CDS             19766..20557
                     /locus_tag="L3M60_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225848.1"
                     /translation="MKLIDEYLDKLYKKCDNKSTIELKQEMRCHLIESANEFKLEGLD
                     EEEACKKAIERFDDGDEMQYELCNIIKELSLSLERHKSIVMWFKKVLGYISIIAFLIS
                     GFMWYYNNNLQHNMYNLGKELDGEIKQLAERHDMTKIDEYKLELEKILDKDKYSKVKA
                     LRLYVIDMKDGNTNLSSSGLNANMVYDREADYNNISNFIQHLGYNGKDFLDKNGNIVN
                     PDIFLEYFFYFESEMLIPVAFALGLLCIIAYFILRFKISLIKNNN"
     gene            20758..21322
                     /locus_tag="L3M60_001161"
                     /pseudo
     CDS             20758..21322
                     /locus_tag="L3M60_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422542.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21534..22727)
                     /locus_tag="L3M60_001162"
     CDS             complement(21534..22727)
                     /locus_tag="L3M60_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBN6225849.1"
                     /translation="MSKVFEVKKDIYFTGVVDEGLKVFDIIMETEFGTTYNSYLIKDE
                     KTVLFDTVKANFKDEFLSNLSEVTDIAKIDYVVIHHTEPDHAGSLKYLLDINPNIEVY
                     CTKAAKLYLDGQINRPFNCHVIKDGEILNIGKRNLRFITAPFLHWVDTMFTYIEEDKT
                     LLTCDAFGCHFASVDADVVNSEDYLKSAKHYYDCIVKPFAKHVLSAVDKVVGLNIEFD
                     TILTSHGPMLTKDPMAAVKRYVEWSTEAVNTTNQNQVSIFYLSAYSNTLEMAKKIKEG
                     LDKEGAKAELYDLEDMTLTEMHDTLVVSKVILLGSPTINKTMVKPMWDLFSVIDPMAN
                     QGKIAGVFGSFGWSGEGITMAETLLKSMSFKMPVESLKKKFFPSEETLKECMAFGAEF
                     AKLVK"
     gene            23046..24890
                     /locus_tag="L3M60_001163"
     CDS             23046..24890
                     /locus_tag="L3M60_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HBN6225850.1"
                     /translation="MNKVDLKKILKKVEKPARYLGNEINSIHKDTSNSELIRYAHCFP
                     DLYEVGMSHLGSHILYDVINKDEDVFCERVYSPAVDMENIMREKSIPLFALESREPIT
                     NFDFVTFTLQYELSYTNILNILDLANIPILKEERTLEDPFIMVGGPCAYNSEPLADFV
                     DVVILGEGEEVNLEVVNEYKEWKKNKTTREDFLYKISSIEGVYIPSFYDVKYNEDGTV
                     QSVTPNREGITKNPTKRIIKDVETVDYPEKLIVPYIDTVHDRIVLELFRGCTRGCRFC
                     QAGMIYRPIREKSVKRLKEILDKLVKNTGYDEISLSSLSTSDYSKLSELTDYLVDEYA
                     SNNIGISLPSLRLDNFSMEIADKIQQVRKSGLTFAPEAGTQRLRDVINKGVTEEDLEN
                     ATERAFEMGWNSVKLYFMIGLPTETYEDLDGIAKLAYKVIDIYRKVNGGKLKRSFSVT
                     VSTSTFVPKPFTPFQWHGQDTTEEVINKQRHLVNKLRNNNIKYNYHDSKTSLMEAVVA
                     RGDRRIGKVIYDAFRLGAKFDGWAEHFNLDIWKEAMEKNNLSIDFYAHRNRNYEEVFP
                     WDHIDVGISKKFLIREDENAKKEKITSDCRHNCNGCGINIHDIGRGLC"
     gene            24892..25593
                     /locus_tag="L3M60_001164"
     CDS             24892..25593
                     /locus_tag="L3M60_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2344 domain-containing protein"
                     /protein_id="HBN6225851.1"
                     /translation="MSKIIRVKFKKEGDMIYISHLDLQRLLQRAFRRAEINLSHSQGF
                     NPHPKMSYGNALALGTESQGEYVDIEIEEDDLSVEEFLNKVSIQLPEGIDFIKAKEID
                     RQTPSLSSVIDYGEYLFNIDLKRPMTKEFVKRKVIDFMNNKEIIITKKNKKGKMVEVD
                     IRPMIRTFDVLNLEDEHITLTATIATGSKINLNTNILIPKILEIFELDIDPLDVDILR
                     RDLYVLEDGELVTPM"
     gene            25611..27032
                     /locus_tag="L3M60_001165"
     CDS             25611..27032
                     /locus_tag="L3M60_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HBN6225852.1"
                     /translation="MKKIVIESLIGSQKTAVLEDERLTELFVEDNLNKKTVSNVYRGI
                     VKKVIPGIEACFVDIGFKKLAYLQLKKGSAIKSGQDILVQINKEEIGTKGAKLNTEIS
                     ISGRYIVYIPSNDRTTISNKITDEKERFRLKKITKAVNKENLGLIIRTEAQGCNHDEI
                     KKDIEELKLKYENILKEYKLGIGPKLLYKSLDFATKYVKDNVNDDIESIITNSYDKYS
                     ELKSILRGIDKTYVDKLCLEENRDVFDLYRIESKIEKLLNKKVWLKSGGYLIIEKTEA
                     LTVIDVNTGKFIGTGKLDETVYRTNLEAAKEIVRQLRIRDIAGIIIIDFIDMHKKKHQ
                     NEVLNILEEEFNKDKRKAEVLGMTKLGLVEVARRREKESIDKYYLMSCPCCDGEQTIK
                     SVHYILDNIEKEIMRISEHTVYKNIIVEFNDFIFEQIKGYYMDIIDKIGEKYNIKISL
                     NANSTLKHNKTNVIFDKIVDNKM"
     gene            27133..27444
                     /gene="rplU"
                     /locus_tag="L3M60_001166"
     CDS             27133..27444
                     /gene="rplU"
                     /locus_tag="L3M60_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBN6225853.1"
                     /translation="MYAIVKTGGKQYKVSEGDVLFVEKLEANAGDVVTLNEVLACSKD
                     GELKLGSPVVEGASVQAKVVEQGKAKKVIVFKYKAKKDYRRKQGHRQSYTKIVVEKIN
                     A"
     gene            27459..27776
                     /locus_tag="L3M60_001167"
     CDS             27459..27776
                     /locus_tag="L3M60_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HBN6225854.1"
                     /translation="MIKVKYYYNDDFLLQGFCLKGHADFAEIGYDIVCAAVTSNAIAV
                     INSLDKLQKIEFEKVVGEEGHIECMVKDTHIKDAQLLLNHFQLAVEEIKREYPKNIKI
                     LKK"
     gene            27790..28080
                     /gene="rpmA"
                     /locus_tag="L3M60_001168"
     CDS             27790..28080
                     /gene="rpmA"
                     /locus_tag="L3M60_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBN6225855.1"
                     /translation="MLNMNLQLLASKKGVGSSKNGRDSISKRLGVKRFDGQLVTAGSI
                     IVRQRGTKIHPGTNVGKGSDDTLFALVDGTVKFERKDKKRKKVSIYPVAIAE"
     gene            28191..29468
                     /gene="obgE"
                     /locus_tag="L3M60_001169"
     CDS             28191..29468
                     /gene="obgE"
                     /locus_tag="L3M60_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HBN6225856.1"
                     /translation="MFIDKARIFVKAGNGGNGSVAFRREKYVPAGGPDGGDGGRGASI
                     IFEVDLGLRTLMDFKYQKKYQAQNGGDGSKGKRAGKNGENLVLKVPAGTVIRDEATGL
                     VLADLKKEGDTAIVAKGGIGGKGNQHFANAVRQAPAFAKSGTDGEERWITLELKMIAD
                     VGLLGFPNVGKSTFLSVVTKAKPKIANYHFTTLTPNLGVVQTKFGDSFVLADIPGIIE
                     GASEGIGLGHEFLRHVERTKVLIHIVDISGLEGRDPIEDFDKINDELKLYNEKLSKRP
                     QVVVANKFDILEDESKFEKFKSELESRGYTVFKMSAATRQGVDDIIAYVSKMLKEVED
                     VELVSEEEMYRPELDIGTEEELSIDIEDGVYVVTGKALRRIMYSVNFDDMESLQYFQK
                     AMESQGVFDRLREMGIEDGDVVKIYELEFEFYN"
     gene            29520..29888
                     /locus_tag="L3M60_001170"
     CDS             29520..29888
                     /locus_tag="L3M60_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HBN6225857.1"
                     /translation="MLKGKQRAYLRSLANTLKPTTQIGKEGVTESFLEQLDGMLRTRE
                     LVKVTILENAGLDAKETANAVCEALRAEFVQAIGFKFTIYKRNIEEPKILFPGHEQAK
                     AKTKNNNVTKKGKPTKRAVR"
     regulatory      29980..30086
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            30189..31394
                     /locus_tag="L3M60_001171"
     CDS             30189..31394
                     /locus_tag="L3M60_001171"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBN6225858.1"
                     /translation="MKIAILGYGTVGSGLIDLIENNASKRDIEVVGILVKNKEKHKYK
                     KYFDKITTDIEDIFDKDIDILVEVIGGLNPAFDYVTRALNKKIHVVTANKDLLAEKGS
                     DLIELANLNDVSIKFEASVAGGIPVLKPLIESLEGNNIKSINAILNGTCNFILSKMYD
                     ENLPYDVALKQAQELGFAEVNPDADVLGYDSARKLSILSTLSYGKIVYWKDLLLEGIT
                     SIDEKDIEYAKKLNCKIKLVARSKYESGEVSGFVRPALVDDNNMLSKIDNEFNVVILV
                     GDSVGELSFVGKGAGRGATGSAVYADIIDIIDNRNSNIKSFSKGKLDLSGLIEDECSA
                     VVRFGGYNNKDGILGCLDKYVDDYDIIDDEELAIFVSAKSEHEIDRFLEDVKRNNYSE
                     SVKKLLKID"
BASE COUNT        12177 a         3479 c         6067 g         9681 t
ORIGIN      
        1 aatttgcatg attaagtata acttaggtat aaatctttta tgcatttgaa agagcttatt
       61 ctttttttga catattcctt taaataattt accagtatct ggtaattgtt tatcatcgtt
      121 caaattttta gcttaatatg atttaaataa atttataata atgatataat ataaaaaaat
      181 aaaaattgga gagtgatttc aaaatgaagt ttaatttttg ccataacaat ttcaatgtta
      241 ctaacttaga aaaaagttta gatttttata aaaaagcttt agggcttaaa gaggtaaagc
      301 gtaaggaagc agaagatgga agttttatat tagtttattt aggagatggc attactagtc
      361 atacattaga attaacttgg cttagagatt gggacagacc atataattta ggagataatg
      421 aatttcatct ggctttagaa gttgaggagt ttgatgaggc aaaaaaactt cataaagatt
      481 tagattgcat atgttttgaa aatgaaagta tgggaattta ttttatagcg gacccagata
      541 attattggat tgaaatactt cctaaaaatc attaatttta aggatatgtt tataatttct
      601 ttacaattaa taagagaata agttatcatt aaattatagg tactaaaaat ggataataat
      661 gttatggatt tatgactata aaaatttgtt ggggggagtg atagtagtga agaaagctat
      721 agcggcttta ggaattgggg cagtagcagt atctgttagc tccataaatg caagtgctct
      781 tgagaaggga acagtaacag caagtgcctt gaacataaga agtggaccaa gctctgattg
      841 cgataaggta gcaaaattgt acaagggaaa aactgtagag attttagaaa aatctaatgg
      901 atggtataaa gtaagggtat caagctctgt tgttggatgg ggaagtgcaa aatacatatc
      961 aacaagtggt tcatctgaag gtacatcaaa tccaaataat tcaacttcaa gtggaacaac
     1021 tatcagtgga aatggtaaag taaatgtgag ctctagactg aatgtaagaa gtggtgcagg
     1081 tactaactat tcattagttg gtaaagcaaa taatggagaa gtagtaaagt tactagaaca
     1141 aagcaatgga tggtataaaa taaaactatc aaatggggtt actggatggg ctagtagcca
     1201 atatatctct aaaacgtcag aagatgttgg agcgaataat tccagtaatt caaatagtac
     1261 taataactct gataaaaaac catctagcga agaatcaatt gaaggtaaaa atggtaaggt
     1321 aacatctact gtgagtttaa atgtaagaag tggtcctggg acaagctatt ctataatagg
     1381 aaaattaaat ggtggagatg ttgttgaatt aaaagctaaa aataatggtt ggtataaggt
     1441 aaaactatcg aatggaacaa ctggatgggt gagtggcagt tatatttctg agactaatga
     1501 aggtacaaaa gaaaattcaa attcatcatc taatcaaaat tctcaatcta ataataactc
     1561 aaagccatca tttactggaa attcagataa atctacagca aaaggttcaa caatcgtaga
     1621 ttttgcatat actcttattg gaataccata tcaatgggga gcttcaggac ctgataaatt
     1681 tgactgttct ggatttacac agtatgtttt taaacattct gtaggcgttt ctattcctag
     1741 agtttctaga gaacaggcta attttggaag tgcaatttct atggggaatt atgcaccagg
     1801 agatttagtt tattttgata ctgatggtga tggaacaact aatcatgtag gtatatatgt
     1861 aggcaatagt aaatttattc attgtagtgg tactcaaact aatcctaata aggttaaagt
     1921 ggataattta actagttcat attggtctaa agtattactt ggtgcaagaa gatttgttta
     1981 gtaagtacaa tttcttatta aaaaattaga ataaataatt atttatattc ataaattgtg
     2041 actcttaaaa atatacttta ttaataatat aattttggga gggacaaact aatgaataga
     2101 agaaaaacga tatacagagg atttaataat aatagacata agattgattt acgtaaatat
     2161 gttataacga ttgcttgctt gtgtctaatt ggctattatt cttatacaaa aataaaagat
     2221 agcaagatat tggagtatgt atcggctaaa ataccatttc taaataattc atcagatata
     2281 acttataaag atatatctga tgaattaaat tcaataaaaa acggaaaaaa atctaaatca
     2341 caaaccaatt cagatgataa acaagaaact aatccagaaa aagcggtaaa taacactaaa
     2401 gagccagaag aagttaaatt agctactata gaaggctggg atatgtacac tattcaagtt
     2461 gctgcaatag ataataatga tgatttgaaa aaaatacaga catcattagt taacaatgat
     2521 ataccttttt cagttatgga gaaggatgga gtaaaaaaaa tacagacata ttcctctttt
     2581 gatgaaaatg atgttagaaa gcagataagc agtgtaagaa aggtgtttcc agatgcattt
     2641 ttatcacact tagatgcacc tatgttatct ttagagtata caagtaacta tgcttatata
     2701 gatagtatat ctaaagaatt aaataaatta ataacaaact ttaaagaaga atcaagtttt
     2761 tggtcgaatg ctgaaaataa tgtagatatg gaaaaatata atactatttt gacaaataga
     2821 aaggcaatat cacaaaatat atcaaaagaa gctgaaaaaa ttgattattc tgaaatgcgt
     2881 ttatttaaag ataatttaat agagtatgta aagaatgtga atgaaaaaat tgatacagct
     2941 tcaaaagcag caaatgaaga aaaatatagt gtaagtaaaa gtctactttt atcatctatg
     3001 caggagtact ctatgtttat aaattctata aaataaaata cagaaaattt ggactttaga
     3061 aagctgaatg aaaaggattg tttaattaag caatcctttt cctcgatttg acttaatata
     3121 gagacattga tttataaaat aaattataaa aatacggata tttttttaac aattgttaag
     3181 attttttaat atatatagta tacttataga aggcagaaaa aatgtttttt tatagataag
     3241 ggggtaaaac aatgaaactc ttaactttta agggagggat acatcctcca tatagaaaag
     3301 agtactctaa cacaaaggca cttgaaaaag ctcaagctcc aaagatagtt tacattcctc
     3361 ttcagcaaca tataggggca ccagctaaaa ctattgttga agttggtgat gaagtaaagt
     3421 ttgggcaaaa aataggtgaa caacaaggtt ttgtttcatg taacgttcat tcttcagtat
     3481 caggtaaagt tattgctatt gaacaacatg aagtaccagg tggttcagct caatgtgtag
     3541 ttatcgaaaa tgactttaag gaagaattac atgagagtgt tcaaccaaaa ggtcagttag
     3601 aggatttgag caaagaagac atagtaggta tcataaaaga ggcaggaata gtaggtatgg
     3661 gtggagctac atttcctaac catgtaaaag tatctcctcc accagatagc aaggcagaag
     3721 tagttatatt aaatggtgca gagtgtgaac catatttaac tgcagaccac agattaatgg
     3781 ttgaaaatcc agaagatgtt gtatttggtt taagagcatt aatgaaggta ttagatgtta
     3841 aaaaaggatt tataggaatt gaaacaaata aaccagatgc tatagaagca attcaaaatg
     3901 tagctaaaga ttatagtgaa atcgaagttg ttggtcttca agtgaaatat ccacaaggag
     3961 cagagaaaca acttatttac gcatgtacag gcaaagaagt tccatctggt ggattaccaa
     4021 tagcagcagg tgcagttgtt gacaacgttg ccacagcagc acaaatagct aagtctatta
     4081 aaacaggtat gcctttggta gaaagaatca ctacagtaac aggtagttgc atcaaagagc
     4141 caaagaacct aataacaaaa gttggaacat tagtttcaga aataatagaa caatgtggtg
     4201 gatttaaaga agataagaag attggtaagg ttataatggg tggacctatg atgggaatag
     4261 ctcaatatac tactgaaata gcgactaaca aaggttcttc aggaatttta tgtttggatg
     4321 aagaagaatc acgtactcca gatgttcaaa actgtttaag atgtggaaga tgtacagatg
     4381 tatgtccatc attcttacaa ccacttttca taagtgcata ttctttaaaa gatgactatg
     4441 acacagctga atatcataga gcaatggatt gtatagaatg tgggtcttgt tcatttattt
     4501 gtccagcaag aagaccatta cttcaatcta taagatcagc aaagagagaa ataggagcaa
     4561 agagaagaaa gcaagctgct cagaaataaa gaaagttaag ggggaaaaac gatggaaaat
     4621 aagttgatag tatcatcttc tcctcatgtg agaagtaatg aagatacttc atatataatg
     4681 aaacaagtta ttatagcact ccttccagca gcagtagcag gagtatactt ctttagactt
     4741 aatgctttga gtgctatgtt tttttgtata cttggtacag taggtactga atttttatat
     4801 caaaaatttg cgaagcaaaa aagtactata ggagattttt cagctgttgt aacaggatta
     4861 ttattagcat ttaacgtacc agcatcactt ccttggtgga tgtgtctagt aggaggaata
     4921 tttgcaatat tagtagttaa aatggtattt ggtggaattg gatgtaactt tgtcaatcca
     4981 gcacttgctg caagagcatt tttattagca tcattcccag tagcaatgac tgcttggact
     5041 caaccaggtg tcaactggat aggtaaaaat ttagatgcag ttactacagc aactccatta
     5101 agctttttga aaaatggagc agcaggacta gcggaccttt ctagcaatgg aattagtctt
     5161 gctgatatga tgatagggaa tattggtgga tgtataggtg agacatcagc aatattatta
     5221 ttattaggtg gagtatacct aatgtataaa ggcataataa attatgttat accagtattt
     5281 tacatagcaa ctgtatttat attaacattc cttttaggtg gatttaatat aactttcgca
     5341 atatatcaac tgtttgctgg aggtcttatg ttaggtggat tcttcatgct tacggattat
     5401 acaacttcgc ctatgactaa aaaaggtcaa ataatatatg ctgtattagc tggtcttata
     5461 acaactgtta taagaatgta tggtggatat ccagaaggtg tatcttactc aatactatta
     5521 gtgaactgtc ttgctccact tatagataag tttgttagaa atagagtatt tggggaggtg
     5581 gctaaataat gaacagtatg gtgagattag gtggtacttt acttgctata agtgcaatag
     5641 ctgcattagc tcttggagct acaaatcagg ttacagctcc agtaatagaa caaagaaata
     5701 tacaagcaaa taatgaactt agaaaagcag tacttccaga agcaaaagaa tttaaagaaa
     5761 tggataaaag tacatataaa gaccttggag atggtcttat agctgaagtg tatgaaggtt
     5821 tagatggttc tgaagtggtt gggtacactt taaaagctaa accaagtgga tatggtggag
     5881 aaattgaagt tatggtaggt atctcatctg aaggtcaagt tacaggtgtt gacattggta
     5941 acatgtcaga aacagcaggt cttggagcga aagctaagga tgatgcattt aaaggtcaat
     6001 acaaaggaaa aactgctgaa cctcttgaag tagcaaaagg aagtacaaca gcagataatc
     6061 aaatattagc aatatcaggt gcaactatta catctacagc agttactaca ggagtaaatg
     6121 ctgctataga tgttttcaat agtgctctta ataaataagg agggttaatt atgaatttag
     6181 ctaaagtatt taaaaatggg ctaatagatg agaacccaac atttgttcaa gttataggga
     6241 tgtgtccaac actagccgtt acaacttcag caataaatgg aattggtatg ggtctttcaa
     6301 cagcagcagt tttaatatgt gcaaatttag ttatatcttt aataagaaaa attacaccag
     6361 ataagataag aatacctata tttatagtag ttatagctac atttgttaca atagtaggta
     6421 tgcttttaaa agcatatgtt cctgctcttg ataaggctct tggtatatat ataccattaa
     6481 tagttgttaa ctgtttaata ttagctcgtg cagaaagttt tgctttcaaa acaggggcta
     6541 tgccttcaat agttgatggt gtaggtcaag ggcttggatt tactgttgca cttacaataa
     6601 taggtgcagt aagagaatta cttggaaatg gtagtttatt tggaatgaca ttatttggag
     6661 catctttcca accagtatta atatttatat taccaccagg tgcattctta acattaggat
     6721 tcttatttgc aggatttaat aaattaagaa gtaaaaaagc atagtggagg tgtaataaat
     6781 gaatttaata cttttatttt taagtatcgt tcttgttaat aacgttataa catctcaatt
     6841 cttaggtata tgtcctttct taggtgtttc taagaaggtt gatacagcag tagggatggg
     6901 agttgcagtt acattcgttt taactctagc ttcaattata acttatttta tacagatttt
     6961 attagtaaaa actggaactg gattcttaca aacgatagct tttatattgg ttatagcttc
     7021 tatagttcag ttcgttgaaa tggtaattca aaagatgagt ccatctttat atcaagcatt
     7081 aggagtttat ttacctctta taactacaaa ctgtgcagtt cttggtatag ctctagttaa
     7141 tgttcaaaaa agttataatt tagtagaaac aataataaat ggatttggag ccggagctgg
     7201 atttacttta gcaatagtaa tattcgcagg tataagagaa agactagaat tagctgatat
     7261 accagaagca tttaaaggtt tccctataac gcttatatca gcaggtttaa tgtcaatagc
     7321 tttcttagga tttacaggac ttataaagct ataaaaaaga ggggtgaaaa tggtgatact
     7381 tacagctgta ttagttttag gaattatggg cttaatcttc gggatagtac tagattttgc
     7441 atctaaaaag tttgctgtag aagtagacga gagagtggaa gctatactag gagtacttcc
     7501 aggagccaac tgtggaggtt gtggatttcc aggttgtgga ggtcttgcaa atgcgatagt
     7561 tgaaggcaat gcaccagtta atggttgccc tgtaggtgga gcagatgttg gtgcaaaagt
     7621 tggagaaata atgggaataa gcgctgaagc aggtgagaaa caagtagcaa aagttatttg
     7681 taaaggaact tgttcaagtg caaaagataa atatgagtat gaaggaatat cagattgtag
     7741 agcagcaaat gttttaaact caggtgccaa gatgtgtaaa tttggatgct taggtcttgg
     7801 aacatgtaaa gatgcttgta aatttgatgc tatttctata gttgatggag tagcagtaat
     7861 agatgaagaa aagtgtgtaa attgtggaaa atgtaaagaa gtttgcccta agggtataat
     7921 aatcacaaaa ccagagagtc aagaagtagt tgtagagtgt aatagcaaag agtttggtaa
     7981 agctgttaag gaaaaatgta ctgctggatg tattggctgt ggaatgtgtg taaaagcttg
     8041 taaatttgat gcaataatat ttgaagacaa aatagctaag atagacccta ataaatgtgt
     8101 aggttgtatg caatgtgtag caaaatgccc tacaaaagta atatcaggtg acataactaa
     8161 gaaaaagaaa gttactattg accaagaact ttgtgttgga tgtacagtgt gcaaaaaaca
     8221 atgtaagttt gatgcaatag agggagaatt aaaagaaaaa cataaggtag atgcagataa
     8281 atgtgtagga tgccatttat gtatggaaaa atgtccaaag aaagctatta aaattttgta
     8341 atatggacaa aataaagata tggaataatt tccatatctc taatgagtac ataaaacaga
     8401 ggtatggaat aatttccata cctctatttt atgtatattt attctaatgg taataaatat
     8461 aacaatagtg taacaaaaaa ggttcgacat ttctgttgaa ttttttacaa tatagtgata
     8521 gactaaatag gtacaaatta cgtacaaatg aggtgaataa atgaatataa tcttggcatc
     8581 agcctctcca agaaggaaag aaattctaga gaatacaaat gtaagatttg atataattaa
     8641 aaatgaaatt gatgagatta tattggaagg tgaaacacca aaacatttag ttatgaggtt
     8701 agcttttgaa aaaagtatgt ctgtagcatc tgaacataat gaagatgttg ttattggagc
     8761 tgatacagtt gttgttttag acaatgctat tttaggaaaa ccaaaagatg agtcttgtgc
     8821 aagagatatg ttaaaaagac tatctggaag agagcatcaa gtcataacag gtattagctt
     8881 aataaacttg tgcgaagata aaaaggtaat tgactatgta atcagcaatg tgaaatttaa
     8941 aacgttatca gagcaagata ttgaggatta tctgaaaact aatgagtcat ttgacaaagc
     9001 aggagcttat ggtatacaag gatatggagc tttgttagtt gaagaaatta gaggagatta
     9061 ttttaatata gttggtcttc caatttcaag gttaagcgac ctattaaaaa aatattttag
     9121 tataaatctt ttttatggag tgtgatttgt ctgaaaaagt catttaattc aaccattaag
     9181 gtaaaagaaa tggcaccaga ggaaagaccc agagaaaaga tgcttgccaa aggggtaaaa
     9241 agcttatcaa atgctgagtt gttggcgata cttttaagaa ctggcgacaa gaataaaaat
     9301 gctattgaat tagccaacta tataataaat agagatattc agggtattag acatttagaa
     9361 gatatgacaa tagaggagct gtgtaatata gatggaattg ggttatcaaa agcaacccaa
     9421 attaaagcag ctttagaact aggctctaga gtagctagtt ttaaacctat aaagtataaa
     9481 ataaggaacc cttgggatat acagagatat tatatggata gcctaaggta tttaaaaaaa
     9541 gaggttttta aagcggttct tttaaacaca aaaaatgaga taatatctga tgtagatgta
     9601 tctgtaggga ctttaagctc gtcattagtc catcctagag aggtttttaa agaagctata
     9661 agaagaagtg caagtaagat aatagtaatg cataatcacc cttcaggaag tgtggaacca
     9721 tcaagagaag ataaaaatat cacatcaaga cttattaaat gtggagaaat aattggaata
     9781 gaaataatag accatataat tattggagat ggattatact ttagctttaa agaaaatatg
     9841 ataatttaac tttatatgtg ataaaataag aagagaatgt tttaaacatg attcttaata
     9901 gtaggaagga gtaaaatcat ggctaaggaa aaaaagaaag aaaaaaaagg tttttttagt
     9961 tttaataaga tgacaaagga tatgggaata gatttaggga ctgcaaatac attagtttac
    10021 ataaaaggac aaggtattgt tgttagagaa ccatcagttg tcgcaataag agatgacagt
    10081 aaagaggtat tagcagtagg tgaagaagcc aaaaaaatga taggtagaac accaggaaat
    10141 atagtagcta taagacctat gaaagatggg gtaatagctg attttgacat aactcaatca
    10201 atgattagtt attttataca aaaagcggct gataaaaaag gtgttgtaag tccaagaata
    10261 gctatatgtg ttccttttgg agttacagaa gtagaaaaga gagctataga agaggctgca
    10321 agacaagcag gagctaaaga tgcattcctt atagaagagc caatggcagc ggctataggg
    10381 gctggattaa aagttgaaga accagaaggt aatatggtag ttgatattgg tggaggtact
    10441 tcagaaatag ctgtaatatc tttaggagga atcgttactg ctaaatctat aagaataggt
    10501 ggagacgaat ttgatgagtc tatagtggct tatgtaaaaa aagaatataa ccttatgata
    10561 ggtgaaagaa ctgctgaaaa tgtaaaaatt aacataggct ctacatttaa agatgatgaa
    10621 gaagtaaata tgcaaataag aggtagagat ttaatctcag gattacctaa aacaatagaa
    10681 atatgctcta cagaagttag agaagcatta aaagaacctg taagttcaat agtagatgca
    10741 ataaaatcta cactagaaag aactccacca gaattagcat cagatataat ggaaaacgga
    10801 ataatgctaa caggaggagg agctttgctt agaggtcttg ataagcttat tacacaagaa
    10861 actggtatgt ctgtacaaat agcagaaaca cctcttgatt gtgttgcact tggaacagga
    10921 aaatctgttg aagaccaaga aatctttgaa aaagtattaa tgatgaatac taagaattag
    10981 aagttggtga tggccttgag atttgacaag aacaaaaatg agaaaaaaag gattaatgtt
    11041 aaagtgatag caacgggcgt agttgctatc actttaattg gaattgtggg tatatcaatt
    11101 ggtaaatttt ctagtggtag cccagtaaat ttaggagtag cttcagatgc tattacaagt
    11161 gtaggtaaag gaataaatga tggattttct tttatcaaaa atggttttaa agacatagca
    11221 aattttaaag ataattctaa aaaggtaaaa aaattagaag aagaaaatga aaaacttaaa
    11281 aaagatatga tatctttaaa tgccaaacta gataaaacag aatcgctaga agagttaaaa
    11341 aagacactta actttgtaca agaagagtat aaggcgacaa gtatttcaac aagtgttgtt
    11401 agtaaaaatg atggaaactg gtatgagagt tttgtcatag gtgcaggaaa aaattcaggt
    11461 gtaaaaaaag aaagtatagt tatgaatggt agtgggttag ttggcatagt ttatgaagtt
    11521 tccaacaatt atagtaaggc aatttcacta ttggattcta aggcatctgt aagctttaaa
    11581 ctagcaaagg atgccaatgc taaaggtaca attactcaaa atactacttt agataataaa
    11641 gatagttata atagtaaagg atatttgcaa ggatatatgt ttgactcatc atataatgta
    11701 attcaaggag atataataac aacatcaggt cttggattct tcccagatgg tataccaatt
    11761 ggcgaagttg aaaaagtggt tgatgataaa gataagtcat taaaatatgt agttgtaaaa
    11821 ccttatgttg attttaagaa tataaatgac gttgtagtca tagaaccaag aaatatagga
    11881 taaaggggat aaaaacaatg aaaaaagttt tactttgtct gttggggatt gtgcttatga
    11941 ctatagaaaa tagtataaca aattatatag atatttttgg cataagcttt aatttagttc
    12001 ttatatatgt aaccataatc tctctttatt tggatgaatt agaaggtggg attatagcag
    12061 caataatagg gcttattaaa gacataaccg ttggtggaat ttttggtgca aatgcattaa
    12121 ttttgtttat tattgcatat gcaattgggt acatgagaga tcagctatat aaaggtagtt
    12181 atattattac tttcgtttta atttttatag gcagtttaat tgattcaatt gtcaatatag
    12241 gtacatctag tataatttat caaagctaca atatatcaac actttttata aaaggtttac
    12301 tagtagcacc aatagtgaat agtttaattg gtttagtaat atatcgctta tctaaaagag
    12361 cagttttaaa acttaaagaa gattagttgg tgatttcatg gaagaaaata aacaagttga
    12421 tagactaaaa ataattaaga tagtcatgag cgtgatattt ttagctattt tagtaaaaat
    12481 aatctatatg actacattta aatatgaata ttacaatgag ttagctgaga ataaaacata
    12541 taaaaagttg gcaatagaag ctccaagagg agaaataaaa gatagatatg gaagactgtt
    12601 ggcaggaaat aaaaacttat ttactgttca agtttcaggt aacgatatta ataaaaaaga
    12661 tgcaaataag cattcaagag caaatgaaat atcattaaaa cttataaatt tgctggagag
    12721 aaatggtgaa gaatatgtag atgagtttcc tatatatgta gaaaatggaa agtactatta
    12781 tacatatgac agagatatta gggaatataa gtcagaaaat ggtattccaa atgattataa
    12841 tgctaaggaa agtttttatt acttggtaga taaacttata tcagctggaa tattatctca
    12901 agaagataaa agattggatg cgacaagatt acaggcaaaa ctaaatgaaa atggatatta
    12961 tccacctatt ttagttagta aatggatgtt cacagcagaa agagataaga gagattggtt
    13021 ggctagttat aagataaaag aaactaaact aagtgccaaa gaagcgtttg aaaaagtaag
    13081 aaatagtgat gcattagaaa ttgataaaaa cttaagtgat gaagatgcaa gaaaaataat
    13141 ggttgtaaga gatttgataa aatcaaaagg atactctcaa tataatccag taactattgc
    13201 gaaggatgta ggagaaacta caattgctca aattgaagag agtgctatgg accttgttgg
    13261 tgtttcaata gcagtagaac cagtaagata ttatcctaat gggtcattag cttctcatat
    13321 gttaggatat gtaggaaaaa tgccatccac tcaaatagaa tcataccttc aaaaggggta
    13381 tgaaactgga gatatggtag gtcttgcagg agtagagaag agtaatgaaa gtagattaag
    13441 aggaactgat ggttataaaa tggttaaagt tgatgcactg ggtagaatca gtaaagaaat
    13501 tgagtctaaa aaacctaaat caggagatac tgtatatctt acactagata aagatttaca
    13561 agaagtttca gataatgcat taaaacagat aatcgaagtt gcttctaagg gtggtacctt
    13621 taaaagtaaa tttggggaca aaccaattag tgcatatgca ggaaaagcac aatcagcagc
    13681 tcttatagct attgatgtta aaaatggaga agtattagct tcatcaagtt atcctaatta
    13741 tgacccaaat aaatttgcaa aaggaatttc tacagaagat tataaagcac ttcaaccaaa
    13801 aaatccaaat gacttactag caggaagtcc acttttaaac ttggttacac aaggggaatt
    13861 tcagccgggt tcttcattta agatggtaac ttcaatggca gcgttagaga atggtttgaa
    13921 tccaaacttt actataaatg accctggggt tataatgtta ggtaaaaaat cttttggtga
    13981 ctatgtatgg aaccatggta gaggaaacca tggtatgaca aacttatata aagcaataca
    14041 agagtcttgt aatatatata tggcaacaat tggtacaggt aaaacatggc cagatggaaa
    14101 aagcattggt atagatatga atgcaaataa aatacttgag tatgcaaaat tatttggact
    14161 tgatcaaaat acaggtcttc aagatgaagt agaagaaaga gcaggtaaag ttcctagcac
    14221 agaagataaa ttgaaatcaa cacaagctct tctaaagtcg aatttagaaa gagaaatggc
    14281 aaatgatttt gttgatataa ctagagaaaa aaatcctaaa gaatatgaga aaagaattaa
    14341 tgagattgta agttgggcag cagaaaagaa aactcctggt agagttgaaa caatgaatag
    14401 gctaaagaaa atgaatgtta aagaagatag aatagaagat gtagcagatt tagcagtatt
    14461 cagttatttt aactttgcaa aatggagtac tgcagataca ttcaacttag ctataggtca
    14521 aggtgaaaat gcttatacac cagctcaaat atcaagatat gtagctgcca tagcaaatgg
    14581 aggtaatcta gtagaattat ctgtagttga tagagctgtt tcaagtgact attcatcagt
    14641 taaaattaat gaccagaaga aagtagaaaa aatacctttt aaaaatccag ataacttaaa
    14701 agaattaaca aaaggtatga aattggttgc aagacaaggt acagctaaga gtgcctttgc
    14761 tgatttccca atagatgtag cagcaaaaac tggtacagct gagaagagtg ggaaaatacc
    14821 tacagataat gagtatgaat atttaaaaag tcatatgtca tcttataatg ttaacctaaa
    14881 tgatgcaatt aaactagctg ataagatgaa agcagaaaaa gaaaaagagc tttctttagc
    14941 taaggaaaag gaaataaaaa agaaattaga aaataaagat ttgaaagacg aagaaagaaa
    15001 aaaattagag gaagaattag aagatggagt aaaagttaga ttagaggaca ccgataaggt
    15061 aaattcttca tatctaagaa aagctataaa agaattaaat cccaaaataa cagatgacca
    15121 aatagataga tttaagcaag attatggttc ttttacatgg actgttgctt ttgctccagc
    15181 agatgaccca gaaatagctg ttgtatgtgt aattcctcaa ggtgattcaa gtgtattctc
    15241 acttttacca actagagaag ttattggaac ttatatggga ttagagccaa ctaatagtaa
    15301 aaatgacaac aagacgaatg atgttaacaa ttcttctgac gaaaatataa atttcgagtc
    15361 acaaattaat agatagctca atccatgtat aaactttagc tttatataga aaaaataaaa
    15421 gggtttaaag cgtatttgaa gaatataaat gatatatcac tctggaggtt gttatgtctt
    15481 taagagaaat ttgtagtcaa gaactagtag agttcaaggg taataaaaga gggataatag
    15541 taaatataaa aagagaagct ccatttgagg aaatacagga aaaaataata aacaaattgg
    15601 aagcatatgt tggctttttt aatggggcta aaattagtaa aattaacagt gattgtttaa
    15661 ctgacatgga aattctagaa ctgaaagaag gaattacttc tagatttgat gtggaatttg
    15721 ttgaagatca aaaagttgaa gaaaatagta attttccaac aaaatatgtc aataccctaa
    15781 gatctggtga aaatattgaa tttgaaggtg atgtagttat attgaatgat atgaaacctg
    15841 gttcaaaggt cctttcaaaa tcaaatacag ttgttatggg agatataaat gctggtgcta
    15901 aggttgtagc aggaggtaat gtttttgtaa tgggcaaaat agaaggtttt gtacatgcag
    15961 gtgcagaagg taatgaattt gcatatgttg ttgctggaaa tcttaatcct aaaatattac
    16021 aaatagcaga taatattgca gaagctccag atgatgaaga aaattatgaa agtgaatcag
    16081 aaattagccc agaaatagcc tttgtaagta atggaagaat tgtaatcgaa agctatttgt
    16141 caaagttaga caaatagaaa gaaaaatagg gggtagtatt atgagcgaag ttatagttat
    16201 aacatctggt aaaggtgggg ttggaaaaac tactaccgca gcaaacttag gaactgcgct
    16261 aagcctagaa aataaaaaaa cagtagtagt ggatgcagat atcggactta gaaacttaga
    16321 tgtagtaatg ggtcttgaaa atcgaatagt ttatgacata gtagatgtag ttgaaggaac
    16381 ttgtaggctt aagcaagctc taataaaaga caaaagattt gataatctat atttattacc
    16441 agcagcacaa actagagata aaaatgctgt ctcagttgaa cagatgattg acctgtgtga
    16501 gaaactaaaa gaatctttcg aatatataat aatagactgt ccagcaggta ttgagcaagg
    16561 ttttaaaaat gcagtagctg gagcagatag agctattgta gttactaatc cagaaatatc
    16621 agcagtaaga gatgctgata gaataatagg tctattagaa gcaaatgaaa taaaagagat
    16681 aagattggtt ataaatagaa ttagaaatga tatggttaag cgtggagaca tgatggataa
    16741 acaagatata atagaaatat tagcaataga tttgttaggt cttgttcctg atgatgaaag
    16801 tataattata tcaacaaata aaggagagcc agctatactt gattctaagt cacttgctgg
    16861 tcaagcatac aaaaatatcg caaaaaggat actaaatgaa gaagttcctc tacttgatct
    16921 tgaagttgaa gatggattct ttggtagact taaaaaaatg tttagcatgg ctaagtagca
    16981 aaggaggata catctgtgtt agatttattt agagtttttt ctaacgaagc taaaactagt
    17041 aagtctgttg ctaaagagag attaaagcta gttttagttc atgatagagt agattgttca
    17101 ccacaacttt tagagatgat aaaaacagac attttaaaag taatagctaa ttatgcagaa
    17161 atagaagatg atggccttga aataaaaatg tctaaatgca gaggtgagca tgatgacaaa
    17221 cctgtatctg cattagttgc taatatacca ctaaagaata taaaagatag atgtatgtaa
    17281 ttagtgctta aaagggggat atccttattg gtatatgccc cttttattaa ttatatgttt
    17341 aaagatagaa agacggtgat tctttgaaaa aaatcaatat taattataag tcgacaatta
    17401 agttgataaa acagttagac tggaaactta tagttacagt attagctatc tttatttttg
    17461 gacttgtcat acttagtagt gcaacacatg ctaattcaac aggttcatat aatcaattga
    17521 ttaaacaagg gttagccttt gtacttggga taggaatgat tatagtcata ttattttttg
    17581 attataattt tctaggcagg tattacaaag ctctttatat aataagtctt gttttgttag
    17641 ccatcgtttt attacctgga attgggtcag taaaaggtgg agctaggtcg tggattaatt
    17701 taggaccact tgacttgcag acatctgaaa tagtcaaatt aacatttgta ctaagttatg
    17761 caaaaatact tgaatccaaa aaggataaat taaatacatt aaaggaagtt atgcctgtta
    17821 tagtttattc tcttccattt attggattat tgattgcaca gccagacttg ggaacaggaa
    17881 ttgtattttg ttgtatgata tttgcaatgc tgtttacagc aggtcttagt tcaaagttga
    17941 taaaaagagg aataatcata ctattggtgt caatgccact gatgtattta atgatggcag
    18001 accatcaaaa ggtaagaata gaagcttttc taaacccaga agatgtaaca ctaaaaggaa
    18061 actatcaagt tatgcaatct ctgattgcaa ttggttctgg tggtgtaact ggtaaaggac
    18121 tttacaatgg aagtcaaaat caagaaaact ttttacctgt acaagatagt gactttatat
    18181 ttgctgttgt aggtgaagaa cttggtgtaa taggaatggc agtgttgata atcttattta
    18241 tgatattcct attaaggctt ttagcaattg caagagatgc aaaagacttt tatggaaccc
    18301 tcatcgtggt tggtgttatg ggaatgtttg gttaccagat aatccaaaat ataggtatga
    18361 cggttgcgtt aataccagta actggagtaa cattaccttt tgtaagttat ggaggtagtt
    18421 cgttattgac atctttagcg aacttagggt tagtactaaa tgtgtgtatg agaagaaaga
    18481 aaataaattt ctaaatttaa atacatctaa acataaattg aggagattag atatatgaat
    18541 atagcattgg tagcacatga ccaaatgaaa aatactatgg tcggattttg tataggttat
    18601 gaatcaattt taaaaaagta tggactatat gcaactggaa ctacaggaaa aagaatcatg
    18661 gatgaaactg aattaaatat aaacagatta gcatctggtc ctttaggtgg agaccaacaa
    18721 ataggttctt taatagtaac acaagaaata gatttagtta tttttttaag agacccattg
    18781 acatctcagg ctcatgaaac agatatacaa gctttaataa gactttgtga tgtttatcat
    18841 gttccaatag ctacaaattt agcttcagca gaaatattta taaaagctct tgatagggga
    18901 gagctatcat ggagagaagt tagaaaaagc aaaagtcagc gtgtttaaaa aatattaata
    18961 ttacagttta aagaaacaga tatataagtg tttaatgcca tagtctttct tttgcaacaa
    19021 acctagtacc tttagaaatc ttatgaaaga tttaaatgat gaggagctaa atttgagatt
    19081 gaataattaa gaaatagatt atagatagag tagtagaatt taaactatta ctctatttta
    19141 ataatatgaa atataattaa gtctattatt atatatattt aaacttatat gtaattctat
    19201 ttccaccaag taaatattgt tacgacaaca ttaataaaat aaaaatttat ttttaaaatt
    19261 tgataaatag ctgtatatta gtatttatag attaaatatt aatatacagc tatttattat
    19321 ttttaagtct attatagaat ataaaccttg tattatatag tactatgtat tattatagta
    19381 aatataatta ctatacatta tagagtagta ggtgaaaagg cggtggtaca taatgcagtt
    19441 aaataaagaa gtgttaaaag gttatatcga tatattaatt gtttctatat tagaaaagaa
    19501 agattgttat ggatatgaaa ttgcaaagca agtaagagaa agaagtgagt ttgaattaaa
    19561 agaaggaaca atgtatcttg cacttaaaag aatggaatct aaaaatttaa ttaaatctta
    19621 ttatagcaat gaacaaagtt caggtggaag aagaaaatat tataatttaa ctaatgaagg
    19681 taaagatttt ctagaaataa agaaacaaga atggagattt attaaaaagg ttatgaatca
    19741 atttttggga gaggtggatt aattcatgaa actaatagat gaatatttag ataaactgta
    19801 taaaaaatgt gacaataaaa gtactataga attaaaacag gaaatgagat gccatcttat
    19861 agaatctgca aatgaattta aattagaagg attagatgaa gaagaagcat gtaaaaaagc
    19921 aattgaaaga tttgatgatg gtgatgaaat gcagtatgaa ttatgtaata taataaaaga
    19981 actatcttta tcattggaga ggcataaatc cattgtaatg tggtttaaaa aagtattagg
    20041 ttacataagt ataattgcat ttttaatttc tggatttatg tggtattaca ataataattt
    20101 acaacataat atgtataatt taggaaaaga acttgatgga gaaataaagc aattagcaga
    20161 aaggcatgat atgacaaaga ttgatgaata taagttagaa cttgaaaaaa tacttgataa
    20221 agataagtat agtaaagtca aagctcttag attatatgta atagatatga aagatggaaa
    20281 cactaattta agttcttctg gattgaatgc taatatggtg tatgataggg aagcagatta
    20341 taataacata agtaatttta tacagcactt aggatataat gggaaagatt ttttagacaa
    20401 gaatgggaat atagtaaatc cagatatttt cttagaatat ttcttttact ttgaatcgga
    20461 gatgttaatt ccagtagcat ttgcacttgg attattatgt ataatagcat attttatatt
    20521 aagatttaaa atttctttaa taaaaaataa taattaaata attcaccaag taattttaat
    20581 ttgtagagta aataagatgt ttcaaaataa aatttgaatc atcttatttt ttattgtttg
    20641 ttatactctt ttattatata tttttccata gtggattgta tctatacata atcacatatt
    20701 ttgatataat tatacttttc aaaaaatgta ctgaatatac aaattataat ctgtataatg
    20761 ttgttagggg agaatgtatg aagtgtatac aactttaatt ttaaatgtat tattgacaat
    20821 actgcaaaga caccgaaaag ctatgtagct gaaaccaaca gtatatatgc tccaaagttg
    20881 ttataagctc aacttaattc agtatcacaa gcaaaagcag actttatgaa taagaaagtt
    20941 acaaagaaca tgaccacaga tgctaagatt agagttgctt acaatcacat taaaaacaac
    21001 acaacatatg ttaactggaa cgcagcagag ggagtttata agcaaaaatt caaatttaga
    21061 cccatcaaag catacaattt ctgtaatagg gtttataaat ataccatatg gtatggttgc
    21121 attagctcca gatggtgaac ctgttagtta tccttttgaa tatgataacc ccttttcttt
    21181 agattttact gctaagaacc tttctacaac agaattatta aaaaagtgta aagaaaataa
    21241 tgtaattatt aaagtagaag ttggtgaaac aacatctaat tctggaatag ggcaagtagg
    21301 atatgctaga tacgtgaatt aatacaatta tactagtata caaatagaac agattataaa
    21361 caatgcaccc tcttggtgag ggtgcattta tcattatatt agtcattttg gaaaataaaa
    21421 aaggagttga acttaaaatt agattagaaa aattaatttt gagacaactc cattttaatt
    21481 tatttttaaa tataaaatca tttattttaa ataatagtat gtgtgattaa tggctattta
    21541 actaattttg caaattctgc accaaatgcc atacactctt ttaatgtctc ttcactaggg
    21601 aagaattttt tcttaagaga ttctacaggc attttgaatg acatagattt aagtaaagtc
    21661 tcagccatag ttataccttc accactccaa ccaaatgaac caaatactcc tgctatctta
    21721 ccttggttag ccattgggtc tataacagag aataaatccc acataggttt aaccatagtt
    21781 ttgtttatag ttggagaacc taataatatt acttttgata ctactagtgt atcgtgcatt
    21841 tctgttaatg tcatatcttc taaatcatat aattcagcct ttgcaccttc tttatcaagc
    21901 ccttctttta tctttttagc catctctaaa gtattgctat aagctgataa gtagaatata
    21961 gaaacttgat tttgattagt tgtattaacc gcttctgtag accactcaac atatctttta
    22021 acagcagcca ttgggtcttt agtaagcatt ggaccatgag aagtcaatat agtatcaaat
    22081 tctatgttta gtcctaccac cttatctaca gcacttaaaa catgtttagc aaatggctta
    22141 actatacaat catagtagtg tttagctgat tttaggtaat cttcgctatt tactacatct
    22201 gcatcaacac ttgcaaagtg acaaccaaat gcatcacaag ttaaaagagt cttgtcctcc
    22261 tcaatgtaag taaacattgt atcaacccaa tgtaagaatg gagcagttat aaatctaaga
    22321 tttcttttac caatatttaa aatttcacca tcttttatta catgacagtt aaaaggtcta
    22381 tttatttgac catctaagta tagtttagca gctttagtac aataaacttc tatattagga
    22441 tttatatcta ataagtattt taaactacct gcatggtctg gttctgtatg atgaattaca
    22501 acatagtcaa ttttagctat atcagtaact tctgataaat tacttaaaaa ctcatcttta
    22561 aaatttgcct ttaccgtgtc aaataaaacc gttttttcgt ccttgattaa ataagagttg
    22621 taagttgtcc caaactcagt ttccattatt atgtcgaata cttttaaacc ttcatctact
    22681 acgccagtaa agtagatatc tttttttact tcaaaaactt tactcatttt taaccctcct
    22741 aaaatttatc attatataag taacataccc cattttaagg tgaataaaac aagagatgtc
    22801 atgataagta atgtaaaaca atagctttat gtttataata tatcatactt tgtaaaaaat
    22861 ttgtatttca ataaaaattt aatagaaaat attcttaaat atttttaact attctatttt
    22921 gtgaataata tgtattttaa attcaatatt ttagttatat ttttgataga aaatatttta
    22981 tcgtgtataa tattacttat aaataagaat aaaaataaag agcttgtttg gaggagtttt
    23041 aataaatgaa taaagtagac ttgaaaaaaa tcttaaaaaa agtagaaaag cctgctagat
    23101 atcttggaaa tgaaataaat tcaattcata aagatactag taatagtgaa ttgataagat
    23161 atgcacattg ctttccagat ttatatgaag ttggtatgag tcacttaggg agtcatatat
    23221 tatatgatgt aataaataag gatgaagatg tattttgtga gagagtgtat tctcctgcag
    23281 ttgatatgga aaatataatg agagaaaaaa gtataccatt atttgcatta gagtcaagag
    23341 agcctataac aaactttgat tttgttactt ttacacttca atatgaactt tcatatacta
    23401 atatattaaa tatattagat ttagccaata taccaatatt aaaagaagaa agaactttgg
    23461 aagacccatt tataatggtt ggtggtcctt gtgcttataa ttctgaaccc ttagcagatt
    23521 ttgtagatgt agtaatatta ggtgaaggtg aagaagttaa tttagaagta gtaaatgagt
    23581 ataaagaatg gaagaaaaat aaaacgacta gagaagattt tttatataaa atatctagta
    23641 tagaaggagt atacatacca agtttttatg atgtcaagta caatgaagat ggtacagttc
    23701 aaagtgtaac tcctaacagg gaagggatta ctaaaaatcc tactaaaagg ataataaaag
    23761 atgttgagac tgtagattat ccagaaaaac taatagtacc ttatatagat actgttcatg
    23821 atagaatagt actagaatta tttagaggat gcactagagg atgtagattc tgtcaagcag
    23881 gtatgatata cagaccaata agagaaaaga gtgtcaaaag acttaaagaa atattggata
    23941 aattagttaa gaatactgga tatgatgaaa tatcattatc atcacttagt acgagtgatt
    24001 atagtaagtt atcagagctt actgattatt tggtggatga atatgcatct aataatattg
    24061 gtatttcact tccatccctt agattagata acttctcaat ggaaattgct gacaaaatac
    24121 agcaagtaag aaagtctggg cttacttttg ctccagaagc aggaactcaa agacttagag
    24181 atgttataaa taaaggtgtt acggaagaag atttggaaaa tgcaacagaa agagcttttg
    24241 agatgggatg gaatagtgtt aagttgtatt ttatgattgg tttaccaact gagacttatg
    24301 aagatttaga tggtatagct aaattagctt ataaagttat tgatatttat agaaaagtaa
    24361 atggaggaaa gctaaagaga agttttagtg ttacagtgag tacttctaca tttgtaccaa
    24421 agccatttac accttttcaa tggcatggtc aagacactac agaagaagtg attaataagc
    24481 aaagacattt agtaaataaa ttaagaaaca acaatataaa atacaattac catgattcaa
    24541 agactagttt gatggaagca gtagtagcta gaggagatag aagaatagga aaagtgatat
    24601 atgatgcgtt tagactaggc gctaaatttg atggatgggc agagcacttt aatttagata
    24661 tatggaaaga agctatggaa aagaataatc tttctataga tttttatgca catagaaata
    24721 gaaattatga agaggtattt ccttgggacc atatagatgt aggtattagt aaaaaattct
    24781 taattagaga agatgaaaat gctaaaaaag aaaaaatcac ttctgattgt agacataact
    24841 gcaatggctg tggaataaat atacatgata taggaagggg gctttgctaa tatgagtaag
    24901 ataataagag taaaatttaa aaaggaaggc gatatgatat atatatcaca tcttgacctt
    24961 cagagattat tacaaagagc atttagaaga gctgaaataa acttatcaca ttctcaagga
    25021 tttaatcctc accctaagat gagttatggt aatgctttgg ctttaggaac agaaagtcag
    25081 ggtgagtatg tagatattga gatagaagaa gatgatttat cagtagagga atttttaaat
    25141 aaagtatcaa tccaacttcc agaggggata gattttatca aagcaaaaga aattgataga
    25201 caaacacctt ctctttcttc tgttatagat tatggagaat atttgtttaa tatagatttg
    25261 aaaagaccta tgaccaaaga gtttgtaaaa agaaaagtaa ttgactttat gaataataaa
    25321 gaaataatca taacaaaaaa gaataaaaaa ggtaagatgg ttgaagtaga tataagaccc
    25381 atgataagaa cctttgatgt tttaaattta gaagatgaac atattactct tactgctact
    25441 atagctacag gctcaaaaat taatttaaat actaatatac ttataccaaa gatattagag
    25501 atatttgaac ttgatattga cccattagat gttgatatat tgagaagaga tttatatgta
    25561 ttagaagatg gggaattagt gactcctatg taagctcgag aggtgaagtt gtgaaaaaga
    25621 tagttattga gtccctaata gggtctcaga aaacagcagt acttgaagat gaaagattaa
    25681 cagaactttt tgttgaagac aatttaaata agaagactgt atccaatgta tatcgtggaa
    25741 tcgtaaaaaa agttatacca ggaatagaag cttgttttgt agatattgga tttaagaaac
    25801 ttgcttattt acaattaaaa aagggaagtg ctataaagtc tggtcaggat attttggttc
    25861 agataaataa agaagaaata ggaactaaag gtgcaaaatt aaacactgag attagtattt
    25921 ctggaaggta tattgtctat ataccttcca atgatagaac tacaatatca aataaaatta
    25981 ctgatgaaaa agagcgattt agattaaaaa aaattactaa ggcagtaaat aaggaaaatc
    26041 taggattaat aataagaact gaggctcaag gttgtaatca tgatgaaata aagaaagata
    26101 tagaagagtt aaaattaaag tatgaaaaca ttttaaaaga atataaactt ggtataggtc
    26161 cgaaattatt atataagtct ttagattttg caactaaata cgtaaaagat aatgtgaatg
    26221 atgatattga atctataatt accaatagtt acgataagta ctctgaactt aagtctattt
    26281 taagaggtat agataagact tatgttgata aactgtgtct tgaggaaaat agagatgtat
    26341 ttgatttata cagaatagaa tctaaaatag aaaaattatt aaataaaaaa gtatggttaa
    26401 agagtggagg ttatttaata attgaaaaaa cagaagctct tacagttata gatgtaaata
    26461 caggtaagtt tattggaact ggtaaacttg acgaaaccgt ttatagaacg aatcttgagg
    26521 ctgcaaaaga aattgtaagg caacttagaa ttagagatat agctggaata ataataattg
    26581 attttatcga catgcataaa aagaagcatc aaaatgaagt tttaaacatc ctagaagaag
    26641 aatttaataa agataaaaga aaagctgaag tattaggtat gacaaagctt ggtttagtag
    26701 aagttgctag gcgtagagaa aaagaatcca ttgataagta ttatttaatg tcttgtccat
    26761 gttgtgatgg tgaacaaaca ataaaatctg tacattatat actagataat attgaaaaag
    26821 aaattatgag aataagtgag cacactgttt acaaaaatat aatagtagag tttaatgatt
    26881 ttatttttga acaaataaaa ggatattata tggatataat tgataaaatt ggtgaaaaat
    26941 ataatataaa gatttcttta aacgcaaata gtacattaaa gcacaataaa accaatgtta
    27001 ttttcgataa aatagttgac aataaaatgt aaatatttta taattaataa ttgtaatgcc
    27061 gcacagtaaa ggtttaaatc ttcaaatgaa gtaccttgta ctggcgagtt tgaggccgag
    27121 gaggtgtatt taatgtacgc tatagtaaag actggtggaa agcaatataa agtttctgaa
    27181 ggtgatgtat tattcgttga aaaattagaa gctaacgcag gtgatgttgt tactttaaac
    27241 gaagtattag cttgctctaa agatggagaa ttaaaattag gttctccagt agtagaagga
    27301 gcatctgttc aagctaaagt tgttgaacaa ggtaaagcta agaaagttat agttttcaag
    27361 tataaagcaa aaaaagatta cagaagaaaa caaggacatc gtcaatcata cactaagata
    27421 gttgtagaaa aaataaacgc ttagtaattg gttaagttat gataaaggtt aaatattatt
    27481 ataatgatga tttcttgtta caaggttttt gtttaaaggg acatgctgat tttgctgaaa
    27541 taggctatga tatagtatgt gctgcagtta catctaatgc tattgcagtt ataaattctt
    27601 tagacaaatt acaaaagatt gaatttgaaa aagtagtagg agaagagggt catatagagt
    27661 gtatggttaa agatacgcat ataaaagacg cacaattatt actaaatcat tttcaattag
    27721 cggttgaaga aattaaacgg gaatacccaa aaaatataaa aattttaaaa aagtaggggg
    27781 tgactccata tgttaaacat gaatttacaa ttattagcat ctaaaaaagg ggtaggttct
    27841 tctaaaaatg gtagagattc tatatctaaa agattaggtg ttaagagatt cgatggtcaa
    27901 ttagtaactg ctggaagtat aatagttaga caaagaggaa ctaaaataca tccaggaact
    27961 aatgttggaa aaggtagcga tgatacttta tttgcattag ttgatggtac tgttaaattc
    28021 gaaagaaaag acaagaagag aaaaaaagtt agtatatatc cagtagcaat agctgagtaa
    28081 tatacataaa aggagtgttg aggcactcct ttttcttata atactgatga atttataatg
    28141 gtatttttgg taatgctaat taaaagtttt taattaagta ggtgaagaat ttgtttatag
    28201 ataaggcaag aatatttgtt aaggctggaa atggtggaaa tggttcggta gcatttagaa
    28261 gagaaaaata cgttccagct ggtggtccag atggtggaga tggtggtcgt ggagcaagca
    28321 tcatatttga agttgattta ggtcttagaa ctctaatgga ctttaagtac cagaaaaaat
    28381 atcaagctca aaatggtggc gacggaagca aaggtaagag agctggtaaa aatggagaaa
    28441 atttagtatt aaaagtacca gcaggtacag taataagaga tgaggctaca ggtcttgtac
    28501 tagcagattt aaaaaaagaa ggagatacag ctatagttgc caaaggtggt ataggtggta
    28561 aaggtaatca acactttgca aatgcagtaa ggcaagctcc agcttttgca aaatctggta
    28621 cagatggaga agaaagatgg ataactcttg aacttaaaat gatagcagat gtcggtcttt
    28681 taggatttcc aaatgtaggc aaatcaacat ttctatcagt tgtaactaaa gctaaaccaa
    28741 aaattgcaaa ctatcatttt acaacactta caccaaatct aggtgttgtt caaactaagt
    28801 ttggagatag ttttgtactt gcagatatac ctggaattat agagggtgct tctgaaggca
    28861 taggattagg tcatgagttt ttaagacatg ttgagagaac aaaggtattg atacatattg
    28921 tagatatatc tgggttagaa ggaagagacc ctattgaaga ttttgacaag ataaatgatg
    28981 agcttaaatt gtacaatgaa aagctttcaa agagaccaca agttgtagtt gcaaataaat
    29041 ttgatatatt agaagatgaa agtaagtttg agaagtttaa gagtgaatta gaaagcagag
    29101 gatatacagt atttaaaatg tcagcagcaa ctcgtcaagg cgtagatgat ataatagcat
    29161 atgtttctaa aatgctaaaa gaagtagaag atgtggagct ggtttcagaa gaagaaatgt
    29221 atagaccaga acttgatatt ggaactgaag aagagctaag tatagatatt gaagatggtg
    29281 tgtatgtagt tacaggtaaa gcacttagaa gaataatgta ttctgtaaat tttgatgata
    29341 tggaatcact tcaatatttc caaaaagcta tggagagtca aggtgttttt gacaggctta
    29401 gagaaatggg aatagaagat ggagatgtag ttaaaattta tgaattagaa tttgagtttt
    29461 acaattaatt taaatataga ttacagataa tttaagtata gaattataga ggtgagaata
    29521 tgttaaaagg gaaacaaagg gcgtatttaa gatctttggc aaatacttta aagcctacta
    29581 cacaaattgg taaagaaggt gttacagaga gcttcttaga gcaattagat ggtatgttaa
    29641 gaactaggga attggtaaaa gttactatat tagaaaatgc aggtcttgat gctaaagaaa
    29701 ctgctaatgc agtatgtgaa gctttaagag ctgaatttgt gcaggctatt ggttttaaat
    29761 ttactattta caagagaaac attgaagagc ctaaaatact ttttccagga catgaacagg
    29821 cgaaagcaaa gactaaaaat aataatgtta caaaaaaggg taaaccaact aaaagagctg
    29881 ttagataata gtttatatcc ttttaaagaa ataagttaaa aaaatagtga caattgggag
    29941 aaattgtatt atactattaa acataaattt aaattagcaa tcttatcaag agtggcagag
    30001 ggactggccc tgtgaagccc ggcaaccagt atatttaatt atacattggt gctaaatcct
    30061 gcagcattta tgctgaaaga tgagtataaa cccgtcatat ggcttgagtt tatactcaag
    30121 cttttttaat tggtatattt atatatataa ataatttaat ataatcaaaa tgatgggggt
    30181 atgttacagt gaagatagca atattagggt atggaacagt aggaagtgga ttaattgatt
    30241 taatagaaaa taatgcttca aaaagagata tagaagttgt tgggatttta gttaaaaata
    30301 aagaaaaaca caagtataag aagtattttg ataagataac tactgatata gaggatatat
    30361 ttgacaagga tattgatata ctagttgaag taattggagg attaaatcca gcttttgatt
    30421 atgtaacaag agcattaaat aaaaaaatac atgtagtaac agctaataaa gatttactgg
    30481 ctgaaaaagg tagcgacctt atagaactag ctaacttaaa tgatgttagc atcaagtttg
    30541 aagcatctgt agcaggtggt attccagttt taaaaccatt gatagaatca ctggaaggca
    30601 ataatataaa gagtataaat gctattttaa atgggacttg taattttata ttatcaaaga
    30661 tgtatgatga aaatttacca tatgatgtgg ctttaaaaca agcacaagag ttgggatttg
    30721 cagaagttaa tccagatgca gatgttttag gatatgatag tgctagaaag ttatctatat
    30781 tgtctacttt atcttatgga aaaatagtat attggaaaga tttattatta gaaggaataa
    30841 cttcaataga tgaaaaagat atagagtatg caaaaaaatt aaattgtaag ataaaattgg
    30901 ttgctagaag taagtatgaa tcaggagaag taagtggatt tgttagacca gcattagtag
    30961 atgataataa tatgctttca aagatagata atgagttcaa cgtagttatt ttagtaggtg
    31021 attcagtagg tgaactttct tttgtaggta agggagctgg aagaggtgca acaggaagtg
    31081 ctgtttatgc agatattata gatataatag ataatagaaa ttcaaatata aagtcttttt
    31141 caaaaggaaa attagattta agtggactta tagaagatga gtgtagtgca gtagttagat
    31201 ttggtggtta taacaataaa gatggaattt taggatgttt agataaatat gtagatgatt
    31261 atgatattat agatgatgaa gaattagcga tttttgtaag tgcaaagtca gaacatgaga
    31321 ttgatagatt tttggaagat gtaaaaagaa ataattatag tgaatcggtt aaaaaattat
    31381 tgaagataga ttaatatttt tatg
//
LOCUS       DAFNNB010000029        30331 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0029, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000029 DAFNNB010000000
VERSION     DAFNNB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30331
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0029"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            73..1185
                     /locus_tag="L3M60_001172"
     CDS             73..1185
                     /locus_tag="L3M60_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5009 domain-containing protein"
                     /protein_id="HBN6225859.1"
                     /translation="MNSRVKSIDIIRGLSIALMIVCNNPGTWMRMYPQLRHAVWHGVT
                     LADFAFPFFVISLGVTIPISINSKLKNNKSTLSIILSIFKRSILLILFGFFLNYLGNP
                     DLNTVRILGVLQRMGLVYFVTSLVYLLLKKLNVRSSVTIITFLCISAFIIVGYYIVAK
                     PYGFELEGSLAQLVDLHFFKGHLYKPDFEPDGFLTSIVAISSGMLGCMMGCVLLKENI
                     GEYKKFFKILVMSIILLIGAFIFNQYFPFNKRLWSSSFVLLMAGSYGILLSIFYFICD
                     IKNKSKIFTPIIALGSSPIFTYMCLEILSHVFWNLPKLTNKVDYPTTLVEWTTYELIT
                     PWAGTTWDSLVFSLLYVLFWVIVMSIMYKKKIFIKI"
     gene            1440..3464
                     /locus_tag="L3M60_001173"
     CDS             1440..3464
                     /locus_tag="L3M60_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6225860.1"
                     /translation="MKKTTKLLATGMVSVAMVAPNVALAAENTTANTESNSDININLQ
                     RKSVVLGSKSNASVKFKEKLNADSITLNFMCYDMPLEATLNYNEKTDSYEGVINYNKD
                     PEYLNVWELQSIKINGKDEQKVLNKEDLESMGLNLKDYDVTQEFIISDANSTKAVNEY
                     MRKTSAPVKKLAGPTRFETAVEISKQGWKDGSSKVVIVNGELAADGITATPLASTYDA
                     PILLANKDDIPESTKAELKRLNPSDVIIIGDDGSVSQKAVSQIKSAVNVNITRIGGVD
                     RHETSLLIAKEIDKYHDVNKIYIANGYAGEYDALNISSKAGEDQQPIILANKDSVPQN
                     TYDWLSSQGLEEAYYIGGSQSLSSKIIDKISKIAKNGTSKNRISGADRHETNANVIKT
                     FYPDKELSAMLVAKSDIIVDSITAGPLAAKLKAPILITPKTYVSAYHSTNLSEKTAET
                     VYQIGDGMKDSVISSIASSLSKHNAPTEPDNSGSAAGKTVVIDPGHGGSDSGATSGLN
                     GGAQEKKYTLNTALATTEYLRSKGINVVMTRDTDKTMALGERTALSNTIKPDLFTSIH
                     YNASNGAGNGVEIFYKVKDKNGGTTKTAASNILKRILEKFNMQNRGIKTRTLANGNDY
                     LYVLRNNNYPAILVECAFIDNKSDMDKLNTTEKVKTMGTQIGIGIEDTVK"
     gene            3684..4370
                     /locus_tag="L3M60_001174"
     CDS             3684..4370
                     /locus_tag="L3M60_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HBN6225861.1"
                     /translation="MSGYTNDECEIPKIKSYPGADKEIASEIDYSIVKGTVLFDLYQR
                     IMDLVLSIIGLVIGLPLIAIFGILIKIEDKGPITYKQERLGKCGRRFYIYKLRSMRTD
                     AEKFGAQWAEKDDPRITKVGKFIRKTRIDEIPQLFNILKGDMGLIGPRPERPNFTVQF
                     NEEIPGFINRLAIKPGLTGWAQVNGGYEITPEEKLKEDIYYIKNRSILLDFKILFKTV
                     KVVLTGDGAR"
     gene            4465..5520
                     /locus_tag="L3M60_001175"
     CDS             4465..5520
                     /locus_tag="L3M60_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp7"
                     /protein_id="HBN6225862.1"
                     /translation="MIKKISTILSLVLLISISSTMWVFADAKPKRELIEGSIPEISTE
                     LNKRAFKDSKEVILVNEDSIVDSISATPLAYAKNAPIVVTKSKNLGRVTRNYLKELGP
                     EKVTIVGGLKAVSKDAERNIEKMGMKVERIRGKDRYDTSLKIAREMYRTVGFDEAFLL
                     SSTTGLENAISVYSYAAKSGMPIIWAKDEGFEEQIDFLKGKNLKKIYALGDSKEFIAE
                     IDSNLKNIEGIKQINKSSTNVDLIKKFYDEKDIKKIYTAKLDFGSRSDVNEYISLGVV
                     SAKENMPILICNDNLSRAQDKFLKDSNINDVVEVGYTVGDYSLFKSIFNLTFLSCIVL
                     ILLLLLITFRALRYESK"
     gene            5636..7192
                     /gene="murJ"
                     /locus_tag="L3M60_001176"
     CDS             5636..7192
                     /gene="murJ"
                     /locus_tag="L3M60_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBN6225863.1"
                     /translation="MSKTAKAALWIMAATMFSKVLGFLRELVLANFYGTGMYADVFVL
                     TLNIPGLIIAVIGSAVATTYIPMYFETKKRLGDEGALKFTNNVLNICYIMAIVIAIIG
                     LLFTEQFVTVFAAGFRNDPAKFQAAILFTKIMISGVLFLSGSKIFSSFLQVNDSFVIP
                     GLIGIPYNIIIIAAIALSAGKNVWILPAGALLAMASQLLFQLPFAFKKSYKYKPYINL
                     KDESIKELVNLVLPMLVGVAVGQLNIFVDRLLATTLGDGKLSALNYANRLNEFVMALF
                     VTSIITVIYPKLAKMSGKDNKEGFISTIVKSSNCIILVVLPISIGAIILAEPLVRILF
                     QRGKFDALSTDLTSIALRLYSLGLLACGVRDVLYRAFYSLSDTKTPMINGSIALIINI
                     VLNLILIRPLGHAGIAISTSTSNIITVILLFISLKKKNGYFGGDKIIKTGLKSLVASG
                     VMAVVTLLIYNNLYAFIGSGTIKEIISVGAGVLGGASVYTILIVLFKVEEMDLAFEFL
                     RKGKQKLLRR"
     gene            7238..8944
                     /locus_tag="L3M60_001177"
     CDS             7238..8944
                     /locus_tag="L3M60_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBN6225864.1"
                     /translation="MDYKNNYEMWLNSPYFDEQTKNELLSIKDDEKEIQDRFYKDLEF
                     GTGGLRGIIGAGTNRINIYTVRRATLGVLNYIIKTQGEEGKQKGIVIAHDSRYMSREF
                     CIEVAKTLSAYGVKAYIFEELKPTPELSFAVRYLKCAMGIVITASHNPKEYNGYKVYD
                     SDGGQICIDVANDIIAEVNKIDDYSVIKSIDFEEALSKNLITILDNEVDDEFIKAVKK
                     QVLRQNIIDEYGKKLKIIYTPIHGTGNKPVRRVLSECGFENVMVVKEQELPDSNFSTV
                     KYPNPEEKSVFNIAIEMAKSNGTDLIIGTDPDCDRVGIVVKDSSGEYIVLNGNQVGSL
                     LVRYIVESLVKENKLPKNNPTIIKTIVTSELGAKIAKAYNVDCLNTLTGFKFIGEKIK
                     AFEKSNDRSFIMGYEESYGYLIGTHARDKDGVVSSLMICEMAAYYSSKGMNLYEALLD
                     TYNKFGYYKEDLKSVTLKGIDGIKKIKEMMLYFRSVKIDNVADVKVDKILDYKEGVDD
                     LPKSDVLKFLLEDGSWIAIRPSGTEPKIKFYFGANSDNQEDVEFKLNNLISYILNVVD
                     SI"
     gene            8966..10039
                     /locus_tag="L3M60_001178"
     CDS             8966..10039
                     /locus_tag="L3M60_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HBN6225865.1"
                     /translation="MKVYNVIMAGGGGTRFWPLSRQEVPKQLINLSGEDALINETINR
                     IDSLAKKDDLFIVTNEKQLEALKDIVKDKCLDRNILPEPCARNTAAAIGFAAFNIMKK
                     YGDGVMCVYPADHYIKEEKEFKSILEKAIYIAENNDKLVTIGITPTFPSTGYGYINFN
                     RENTIEDVAYEVVEFVEKPNYEIAKEYVNSKKYVWNSGMFVWKVSKILEDFKRYLPKV
                     YEKLEEISKYLGTKEEVEKIKEIYPTIQSISIDYGIMERSNDVIVVPGDFGWNDVGSW
                     DSLGAIYPTDDEGNIKRGENITIDTKNSIIYSDDKLISTIGISDLIVVSTNDAVMVCR
                     KDKAQDVKKIVEQLKEEDRQEYM"
     gene            10051..10773
                     /locus_tag="L3M60_001179"
     CDS             10051..10773
                     /locus_tag="L3M60_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBN6225866.1"
                     /translation="MIPKKIHYVWFGGPKGNIENICINSWKEKLPEYEIVEWNEKNFD
                     IEKEIKGNKFLEECYKRKLWAFISDYTRIRVLYEQGGVYMDTDMQILKDITPLLEDNR
                     LICGYEDDGEYMNAAILGVEKGHPFFKDLLEYYEKEILTSSLFTIPKIMTHLMEKNYK
                     KIDQNNYEEGIRVYDKEYFYPFGFKEDFTPECITENTYGIHWWGKSWAKKRNYFLESK
                     HLTGVNKIWKCCKIFASNTLRS"
     gene            10789..11946
                     /locus_tag="L3M60_001180"
     CDS             10789..11946
                     /locus_tag="L3M60_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide polymerase"
                     /protein_id="HBN6225867.1"
                     /translation="MKTLNDDRNIMIRKYLYIFVLFIILLRKYMISFIDPNMDIGMIK
                     SALFYSSIGILMLLFLFDKRKSITEMVLVGVCVLLYLLNREGAILLIVLLAVSAKQID
                     DKFIVKNYLIISACFLMVAILLFNLFPSLIFNQEVHYRYIEKIDMLVTRMDFGLGNPN
                     SVFYHMVTIYAAYIFLRYKDYNKWDRIILFGSAFFVYQTTYSRTGFFTILAGLIFVEI
                     IRWIDIKKIKGLPMLLKTLPIILTLFSVIIGTVFDKSTLLNRLLASRPKFWNVYLAEK
                     GNFLKPFGNSYSPAIKATNPLDSSYVYIISVLGVVACILFMYLMYKGIESFIEKDKKA
                     YLVALFIFLLYSFAENLLLEASFSFAVVLLIKEVILNDKREIDLWKMKSRR"
     gene            11949..12788
                     /locus_tag="L3M60_001181"
     CDS             11949..12788
                     /locus_tag="L3M60_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6225868.1"
                     /translation="MLISLIMPTLNRYDDIYLLMDSLENQTYKNFELIVVDQNDNSKV
                     KEIVDKYIDKLDIKYIKSSKKGLSYNRNVGIDNAVGQIIGFPDDDCVYENDTLEKVIN
                     FFNKNKDYKIYSCKTMDANKIDAFKKMYDGTCDITSSNVLDTITSITFFIDFEGKDYT
                     RFDEKLGVGGEFGAGEEIDYVLNLLSLGFKGKYFGDDIIYHPAKKHSKSKEKYQKDYN
                     YGRGFGALCKKEIVYRKNYKFVKVMVSKLVRNIGGLILSSNRDYHSATIKGRINGFRQ
                     YKL"
     gene            12803..13978
                     /locus_tag="L3M60_001182"
     CDS             12803..13978
                     /locus_tag="L3M60_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HBN6225869.1"
                     /translation="MDKVKFLINMLLAFFIYPFNKHRFKGRNIWLIGGHSGDIYNDNS
                     KFFYEYMLKEHNDVETYWVVNKDSKVFDKIPGKKLIRGSVENYLYYYNSKAIVFSHAP
                     SADIAPYNFAVPVLNYFHKKTIKVFLNHGTISFKKRKPMNKKFKNIIDNLYKSYNIVT
                     ASSEFERNVMVNDWGMLDDSVYIIGNARYDNLPTNEVAQTRDILYTPTWRDWIKFSSG
                     KFTDTDYFKNIMNFLNDDKLNKILDEKDINVKIYMHHLMHEFIDDIKENITGKRIVFL
                     DKGVTLANEIRKSAANITDYSSVAIDFLYMNRPILFYQFDLDEYMEKVDSYIDLKSEM
                     FGSLAYNNDEAVNKLIDIIENNFEVMDNQKNERNKFFRYNDNKNCKRIYDCVLSKIK"
     gene            14007..14756
                     /locus_tag="L3M60_001183"
     CDS             14007..14756
                     /locus_tag="L3M60_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6225870.1"
                     /translation="MKKNLVSIITPMYNSEKFIEATIKSVLNQTYQEWEMLIIDDCST
                     DNSPNIVKSYMQQDSRIKCIKTETNKGVSNARNLALSKATGQFIAFLDSDDQWNSSKL
                     EKQVNFMLENDYVISFTSYELMDENDKKLNKVIKVPPNVDYRRLLKGNILGCLTVVID
                     KSKLDFEIRMSGVRHEDYVLWLSILKKGHIAHGINEVLALYRKSSNSLSGNKIKAAMW
                     TWNIYRNIEKIPLYKAIYYFINYGINGIKKS"
     gene            14778..15686
                     /locus_tag="L3M60_001184"
     CDS             14778..15686
                     /locus_tag="L3M60_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6225871.1"
                     /translation="MYKYKFTVFTPTYNRAHLLENLYNDLKAQTYDFNDFEWLIVDDG
                     SSDSTKELVEKFISENKLNIRYIYKENGGKHTAINLGVKNADGELFFIVDSDDGLIKD
                     SLEIANDEWDSLENKEGFSGVVGLCVYPSGDLIGTEMPEDKKICHFADLYFKYGVKGD
                     KSIVFVTDELIKFPFPERKEVRFLPESVVWNEMSKYYKVICVNKPMIIRDYLDDGLTK
                     NILSKNALKGRALEFLYLINQNTYPLSRYPYMWIKNYINLARYSLLSDSHYFGELRKV
                     SDKLLYLALFPLGYYKYIGQRKLVSK"
     gene            15734..16486
                     /locus_tag="L3M60_001185"
     CDS             15734..16486
                     /locus_tag="L3M60_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6225872.1"
                     /translation="MNEPLVSIITPVYNSEEFLSETIKSIQNQTYKNWQLLLVDDCSK
                     DNSSEIIKSFRKEDARIKYIKLEKNSGAAVSRNVGIKNAEGRFIAFVDSDDLWDSRKL
                     EIQIEYMLKENIGFSFTSYRYMRQDGSKTNKVARAPKKIDYEGLLRNTIIGCSTVVID
                     KEIVGEFSMPLVRRGQDTATWLQLLKKEKYAYGIQEDLVNYRLVGNSISSNKIKALKR
                     TWNTYRNVENLSLPKSLYVFCFYVFNAIKKRV"
     gene            16464..17807
                     /locus_tag="L3M60_001186"
     CDS             16464..17807
                     /locus_tag="L3M60_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HBN6225873.1"
                     /translation="MPLKKEYKFYILGGKKMKIAIAGTGYVGLVTGVCLAEVGNENVV
                     CVDVDEQKVKSMKLGIAPIYEEGLEELMVKNYNRGRIDYTTDYKKAYEDVDVILIAVG
                     TPERSDGSANLDYIKAVAKQIAETVTKDTLVVIKSTVPIGTNEEIEDYINRNLKNDVK
                     VELASNPEFLSQGTAVKDTLHASRIVIGVESEWARDILQQIYEPFNQPIIVTNRNSAE
                     MIKYASNNFLALKISFMNDIANLCEIAGANIEDVALGMSYDDRIGSKFLKAGIGYGGS
                     CFPKDTKALHWLSEHNGYELKTVKAAIEVNESQKLKLVRKASKLVESFQDMKVAVLGL
                     TFKPDTDDIREAPSIPNIKYLLERGARVTVYDPIGVNNTKKIFGDSILYADSVDDAIE
                     EAEICFIMTEWKDVLNYDIHKYKEKMKNPLVLDGRNCYKVKDMEELGIEYYSIGR"
     gene            17813..18904
                     /locus_tag="L3M60_001187"
     CDS             17813..18904
                     /locus_tag="L3M60_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBN6225874.1"
                     /translation="MKTVCYLADGSNPHTLKWCSFFKNKGYDIHVISLNGGHMEGIKI
                     YNFGSNVEELKNENISKKTGYLGSVKQIKKLVNEIKPDILHAQYASSYGFIGSLLGYH
                     PYIISVWGTDIYDFPNNGFIQKQIIKHNFRKADYIFSNSRDMAKEANKYTAKHVDVTF
                     FGVDMDRFKPMEVEKEDAFVIGIIKSLEKKYGIEYLIQAFKMLKDEYKDKKIILKIGG
                     SGSQMDNLINLTKELGIEDDVQFLGRISPENVSKTFNSFDVTVFPSLREGFGVAAIES
                     EACEVPVIVTNVGGHPESVWENETGLIVEPKQPEEIKNAIIKLMENDELRLSMGKKGR
                     QFVRENYEVNLNFNDIEKIYDSIFDKYKK"
     gene            18987..20900
                     /locus_tag="L3M60_001188"
     CDS             18987..20900
                     /locus_tag="L3M60_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HBN6225875.1"
                     /translation="MRESIDLEKESFFNRLFKKYKFRHLTLLILDICSVLVAFRMSLS
                     LTGNLNVFTTNDIIISACIYIVLHIVSFRLFKCYNTLWRYAGEEEIISIFMATLAYLI
                     PVYIINKLLGFDYPIIFYVLNTIFIIMFTSGARIAYRAIRIVMNKTYSRGKISNILII
                     GAGDAGEMVIQELKRNPELKKVAVAIIDDDKNKIGRIIHNVKIVGTTSNIKEVVEKYN
                     VDEIIFSIANIEKRRKKEIIDICKNTNCKIKTIPGIYEIIDGKVDIKQIREVDIEDLL
                     GREPIKTNLREISNYIEGKVILITGGGGSIGSELCRQIAGFNPKELIIVDNYENNAYS
                     IQQELLRKYKNKLDLKTVIASIREEKRMDEIFNKYKPEVVFHAAAHKHVPLMESSPGE
                     AIKNNIFGTLNIAGLSSKYRAKKFVLISTDKAVNPTNIMGATKRAAEMIIQTMNAESQ
                     TEFVAVRFGNVLGSNGSVIPLFKKQIEDGGPVTVTHPDIIRYFMTIPEAVGLVIQAGA
                     MAKGGEIFVLDMGEPVRILDLAKNLIKFSGFEPDVDIKIEFSGLRPGEKLYEELLMSE
                     EGLLDTEHKKIFIGRPIDVDREKITKYLKLLREITNNEEVEKIDGIMRELVPTYIKPE
                     DANIKEIATTREK"
     gene            21088..21795
                     /locus_tag="L3M60_001189"
     CDS             21088..21795
                     /locus_tag="L3M60_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBN6225876.1"
                     /translation="MMNIKNKKHILKKFIAMVLIAGVVTVEAGAITASAAELTNSPMS
                     ATVDQCDFLNVRSGASANDAVVGKINTGDKVEVLELHSNGWIKIKTVDNVTGWVNGDY
                     LTIQGGNVDAKVQNVLNLAFKQQGKPYRWGATGPNSFDCSGFTSYVYKNGAGVNLPRV
                     SRSQATVGKKVSRAELKPGDLVFFGSGGSINHVGLYVGNSKFIHSPQTGDVVKVTSMA
                     PGTNYARRLITATRVLQ"
     gene            22096..24207
                     /locus_tag="L3M60_001190"
     CDS             22096..24207
                     /locus_tag="L3M60_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding glycosyl-hydrolase Cwp19"
                     /protein_id="HBN6225877.1"
                     /translation="MKKISILVLSLIMTLTMCSVSSFADSSNDKEMRAAWISTVYNLD
                     WPKTKNNEAKQKKEYTDLLDKLKSVGINTAVVQVRPKSDALYKSNINPWSEYLTGTQG
                     KDPGYDPLPFLIEEAHKRGMEFHAWFNPYRITMADESIDKLPANNPAKKNPSWVVKHG
                     NKYYYDPGLPEVRKYIVDSIAEVVQNYDIDGVHFDDYFYPGVSFNDTATYQKYGNGQN
                     KDNWRRENVNTLLRDVKASIKSIKPNVVFGVSPAGIWRNKSSDPTGSDTSGNESYVGT
                     YADTRAWIKQGLIDYVVPQLYWPIGLKAADYSKLVSWWANEVKGTNVDLYIGQGIYKQ
                     GQSSYGGQNIAKEIVQQVTLNRKYSEIKGSMYFSAKDIANSSSIQKDLKSLYSSSEEP
                     VTPPSNVKVEKLRGDERYDTAVAISKKGWPTNSDTVVLVNGYSIVDGITSTPLATSND
                     APILLVNKDNIPTSTKNELKRLNPSKVILIGGNSSIGDKVESEIKDTLSNVSINRVGG
                     SDRYSTSLMIAKELVKNNPVEKLYITSGTGEADSLSIASKAGEEKQPIVLVSKDNVSD
                     EVYNWISDLKVKDAYFIGGNLSISDSVINKLDKVITNDVSKNRIAGENREETNGKVIQ
                     KFYPNAEYSSMFVSKSNQLVDALTSGPLAAKLKSPVVMLGNSVTSAQKTALEPKKTNL
                     VYTAGDGINQNTLNAFLNLVN"
     gene            complement(24405..25370)
                     /locus_tag="L3M60_001191"
     CDS             complement(24405..25370)
                     /locus_tag="L3M60_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBN6225878.1"
                     /translation="MSKLKKFVILLAFLVVIFPISVYGYFYYKLSAIHDSSISSDLLD
                     NNDHKNEDGIINILLMGTDARPNEDSSRSDAMMILTIDNKHNDIKLTSLARDSYVDIP
                     GHGKQKLTHAYAYGQADLLIQTIEENFNIDIQNYACVNFESFMYIIDAIGGVEVTIEK
                     GEIRELNKFIPETYKWNKSDDKGSIQYIRNAGKQTLNGYQALSFARIRHNDTAFARDG
                     RQRQIIQAIIKKTETLPVTKYPGLLDAVLPYVKTNMKPNAILSLGAQVLKMGDLNIKQ
                     FEFPIDDEIHSTGGIYGKAGWVLRFDPDTLDILHDFIFNDIEFKQ"
     gene            complement(25655..26611)
                     /locus_tag="L3M60_001192"
     CDS             complement(25655..26611)
                     /locus_tag="L3M60_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HBN6225879.1"
                     /translation="MSGLKKFVIFLALLVIAFPVSIYGYVFTKLKTIQDDSINTEALS
                     SNDYKNEENITNILLLGTDARPGEEVSRSDAMMILTIDNKNKSLKLTSLNRDTYVNIP
                     GRGEQKLTHAYVYGQANLLIETIEENFQLDIQNYAVVDFFSFMDIVDALGGVTVTVDK
                     SEIRELNKFIPETYKWNKNDNKGSLKYIRNSGEQKLNGYQTLAFSRIRKNDSTDERDR
                     RQRSVIQSLINGVKDLPVTKYPSLVNTILPYVKTNMNPNEIISLGKELLSIGNLDLKS
                     MEFPLSTENGRKIGNAGYVIPFEEYELDAMHDFIFKDIMVED"
     gene            26980..27768
                     /locus_tag="L3M60_001193"
     CDS             26980..27768
                     /locus_tag="L3M60_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBN6225880.1"
                     /translation="MIKLIATDLDGTLLDEKSEINPEFYKVFKKLRERGIMFSAASGR
                     QYQNLIKKFEAIKDDMMFISENGTLVVYKGKEILSNPLDKELVNEIIETTRSIEGKKI
                     VMSGKKYAYIESKDEAFIDEVSTYYAKFKVVDDLTKVEGDILKIAIFDFKGAEHNNSI
                     YFEKFSDRAQVCISGVEWLDLTAKGANKGSAIKKVQKMLDIKYEETMVFGDQLNDVEM
                     MKSAYHSYAMENANEYLKEIARFRAKRNTENGVVDKIKEVMKIG"
     gene            complement(27899..28369)
                     /locus_tag="L3M60_001194"
     CDS             complement(27899..28369)
                     /locus_tag="L3M60_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HBN6225881.1"
                     /translation="MKKILILFFSIILSLGMVSCSSSGDYKNVSTSKIKKALKSSDLL
                     IKESKIFDAKDFNYFNDVEEYIIQGFVINSSEKLSLEDIIVIKTDEVDKIYKTLENYK
                     KDMIQAPFGEGHGEKINSEIANNTIIEKAGRYVYLISAENAPQIENKILSIIKK"
     gene            28508..29152
                     /gene="uppS"
                     /locus_tag="L3M60_001195"
     CDS             28508..29152
                     /gene="uppS"
                     /locus_tag="L3M60_001195"
                     /EC_number="2.5.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-trans,poly-cis-decaprenylcistransferase"
                     /protein_id="HBN6225882.1"
                     /translation="MRIPKHVGIIPDGNRRWASQNGMTKDKGYESGIDPGLESYRLCR
                     DLGCEEVTFYGFTTDNTRRPSVQTKAFTKACIDAANILIDEGAALLVVGNTKSPMFPE
                     ELLPYTNRIKAKEGQIKANLLVNYGWYWDLNNISGVENVNKNNIQDYIQSFDVSRLDL
                     IIRWGGRRRLSGFLPVQSIYADFYVIDNYWPDFKSDDIKDAFSWYDTQDVTLGG"
     gene            29195..30100
                     /locus_tag="L3M60_001196"
     CDS             29195..30100
                     /locus_tag="L3M60_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6225883.1"
                     /translation="MKKKLLDGKITLIICKSVKIYTKKGEEMSKGNNNNNSRNKSKKT
                     SHLNRKKRKLNKKKLAVLICFTVLFLFIAFKVTQGVVALVKSMDKGNKTSQQQNVNSE
                     QFDLGNEEENKNKKYTVFIDPGHGGNDKGTESKTSHRYEKDLNLQIAKKLANKLSKQK
                     DIQVVVSRTDDTYISLKDRAILANNSSADVLVSIHLNAEKNGNTATGIETWYRNKATD
                     GSKELAQTVQSTIVSYVKVRDRGIVENNFEVLRESNMPAILIECGFLTTPSDEQKIIN
                     EKYQEQLAEGIVQGVLSYLDSKGNK"
BASE COUNT        11994 a         3301 c         5400 g         9636 t
ORIGIN      
        1 aatagcaata gcaatagcaa tagcaatagc aatagcaata aaatttcaaa taatagtata
       61 gattctaaat ttgtgaatag tagagttaaa tcaatagata taataagagg gctatcaata
      121 gccctcatga tagtttgtaa taatcctggg acttggatga gaatgtatcc tcaacttaga
      181 catgctgtat ggcatggtgt aacacttgca gattttgcat ttcctttttt tgttatatcc
      241 ttaggtgtta caattccaat atcaataaat agtaaactaa aaaataataa atctacatta
      301 agtataatac ttagtatatt caagagaagt atcttattga tattatttgg atttttctta
      361 aattacctag gaaatcctga tttaaatact gtaagaatac ttggagtact tcaacgtatg
      421 ggactagtct attttgtgac tagcttagta tacttactac taaaaaaatt aaatgttaga
      481 agttctgtta caataattac tttcttatgt atatctgcat ttataatagt aggatattac
      541 atagtagcaa aaccttatgg gtttgagtta gaaggttctc ttgcacaatt agtggattta
      601 catttcttta aaggtcattt gtacaagcca gattttgagc cagatggttt tttaactagt
      661 atagtagcaa tttcttcagg aatgttggga tgcatgatgg gatgtgtgct tttaaaagaa
      721 aacataggag agtataaaaa attctttaaa atattagtta tgagtattat tttactaata
      781 ggtgctttta tatttaatca gtattttcca tttaataaaa gattatggtc tagctcattt
      841 gtacttttaa tggctggaag ttatggtata ttactgtcaa tattttattt tatatgtgac
      901 atcaaaaata aaagtaaaat atttacccca ataatagcac ttggaagtag ccctattttt
      961 acatatatgt gtctcgagat attaagccat gttttttgga accttccaaa acttacaaac
     1021 aaagtcgatt atccaactac cttggtagag tggactacat atgaacttat aactccttgg
     1081 gcaggtacaa cttgggattc tcttgtattt tctttattgt atgtcctatt ctgggttata
     1141 gtaatgtcta ttatgtataa gaaaaaaata tttatcaaaa tttagtgcaa tatttttaga
     1201 cgctaaagtc tttgttttgt ttaaatatac ggaaactatc aaatataatc aaaatttccc
     1261 ctcgataaaa tttaaaaacc atgccataaa agattacaaa aagtttacaa tttgtaattt
     1321 tttgtgctat aatgaaaaaa gatttacata atattgaatt tatgtaatat aaaacaagct
     1381 taagatgtat taaaaaagat ttaaagattt aaaggtccaa aaaatttagg ggggatatga
     1441 tgaaaaaaac aacaaaatta ttggcaacag gaatggtgtc tgtagcaatg gtggcaccaa
     1501 acgtagcttt agcggcggaa aatactactg caaatacaga atctaactca gatattaata
     1561 taaatttaca aagaaaaagt gtagtgttag gaagtaaatc taatgcaagt gtaaaattta
     1621 aagaaaaatt aaatgctgat tcaataacat taaattttat gtgttatgat atgccacttg
     1681 aagctacttt aaattacaat gaaaagacag attcatatga aggtgtaata aactataaca
     1741 aagacccaga atacttaaat gtatgggaac ttcaaagcat aaaaataaat ggcaaagatg
     1801 aacagaaagt tttaaacaaa gaagacttag agtctatggg attaaatctt aaggattatg
     1861 atgttacaca agagtttata ataagtgatg caaattcaac aaaagcagta aatgaatata
     1921 tgagaaaaac ttctgcacca gtaaaaaaac ttgctgggcc tactagattt gaaactgctg
     1981 ttgagataag taaacaaggt tggaaagatg ggtctagtaa ggttgtaata gttaatggag
     2041 aattagcagc agatggaatc acagctactc cacttgcatc tacatatgat gcaccaatat
     2101 tacttgcaaa taaagatgat ataccagaaa gtacaaaggc agaattaaaa cgtctaaacc
     2161 caagtgatgt aataatcata ggtgatgatg gttcagtttc acaaaaggca gtatctcaaa
     2221 ttaaatctgc tgtaaatgta aatataactc gtattggtgg agttgataga catgaaacat
     2281 ctttacttat agcaaaagaa atagataaat atcatgatgt taataaaata tacatagcaa
     2341 atggatatgc tggtgaatat gatgctctta acatttcatc aaaagctggt gaagaccaac
     2401 agcctataat attagctaat aaagattctg taccacaaaa tacatatgat tggttatctt
     2461 cacaaggttt agaagaagct tattacatag gtggtagtca aagtttatca tcaaaaatta
     2521 tagacaaaat atctaaaata gcaaaaaacg ggacttcaaa aaatagaatt tctggagcag
     2581 atagacatga aacaaatgca aatgtaatta agactttcta tccagataaa gagttgagtg
     2641 caatgttagt tgcaaaatct gacataatag ttgattcaat aacagcagga cctttagcag
     2701 ctaaattaaa agctccaata cttataactc caaaaactta tgtttctgca taccatagta
     2761 caaatttatc agaaaaaaca gcagaaacag tatatcagat tggtgatgga atgaaagata
     2821 gtgttataag ttctatagca tctagtttgt caaaacataa tgcaccaaca gaaccagata
     2881 actcaggttc agcagcagga aaaactgtag tgatagaccc aggtcatggg ggttctgatt
     2941 caggggcaac aagtggacta aatggtggag cacaagaaaa gaaatatact cttaatacag
     3001 cattagcaac tactgaatat ttacgttcaa aaggtataaa tgttgttatg actagagata
     3061 cagacaaaac aatggcttta ggagaaagaa cagcattatc aaatacaata aagccagatt
     3121 tatttacaag tatacattac aatgcatcta atggagctgg taatggcgta gaaatatttt
     3181 acaaggtgaa agataaaaat ggtggaacaa ctaagactgc agcatcaaat atattaaaaa
     3241 gaatactaga aaaatttaac atgcaaaata gaggaatcaa gacaagaaca cttgcaaatg
     3301 gaaatgatta cctatatgtg ttaagaaata ataattatcc agcaatactt gttgaatgtg
     3361 catttattga caataagagc gatatggata agttaaatac tacagaaaaa gtaaaaacaa
     3421 tgggaactca aataggcata ggaatagaag atacagtaaa ataaaataaa aaatattaaa
     3481 gcaaaacata tcatctcctg ttataatgta aaatataaca agagatgata tatttttgta
     3541 aagaatcaaa ttgtttacaa tgatgtttct tgtattgata atctgctcat tattgagtat
     3601 aatatagaaa taagtcaaaa ataagataga aataagttga aattaagaat taaaaaaatt
     3661 tttattccaa tatggaggta actatgtctg gatatactaa tgatgagtgt gaaataccta
     3721 aaattaaaag ctatccaggg gcagataaag aaattgcaag cgagattgac tatagcatag
     3781 taaaaggaac agttttattt gacttgtacc aaagaattat ggatttagtt ttatctataa
     3841 taggacttgt gataggttta cctttaatag caatatttgg aattcttatt aaaatagagg
     3901 acaaaggacc aataacttat aagcaagaaa gactaggaaa atgcgggaga agattctata
     3961 tatataaatt aagatctatg agaacagacg cagaaaaatt tggcgcacaa tgggctgaaa
     4021 aagatgatcc tagaataact aaggttggaa agtttattag gaaaactaga attgatgaga
     4081 ttccacagct gtttaatata ttaaaggggg acatgggctt aataggtcct agaccagaga
     4141 gaccgaactt tactgttcaa tttaatgagg aaatacctgg atttatcaac aggctcgcta
     4201 taaaacctgg gcttacgggt tgggcacaag taaatggtgg atacgaaatc actcctgaag
     4261 aaaaacttaa agaagatatt tactatataa aaaataggag tatattactt gactttaaga
     4321 tactctttaa aactgttaaa gtagtattga caggagacgg agctagataa ttttaaaata
     4381 ttagattact aatcaatgaa aaagctcttt aaaaagagct tttttattgt caaaaaatac
     4441 aaaaaagaaa aggaatgaga acttatgatt aaaaaaattt ctactatatt atcgttagtt
     4501 ttgttaatct ctatttctag tactatgtgg gtatttgcag atgcaaagcc caaaagagag
     4561 ttgatagaag ggagtatacc agagatttcg acagaattaa ataaaagagc atttaaggat
     4621 tctaaagaag taattcttgt aaatgaagat tcaattgtag atagtataag tgcaacacct
     4681 ttagcatatg caaagaatgc tccaatagtt gtaactaaga gtaaaaatct tggtagagta
     4741 acaagaaatt atctaaaaga actaggtcca gagaaggtta cgatagtagg tggtttaaag
     4801 gcagtatcta aagatgctga acgtaatata gaaaaaatgg gaatgaaagt agaaagaata
     4861 agaggaaaag acagatatga tacatcttta aagatagcta gagaaatgta cagaacagta
     4921 ggatttgatg aagcattttt acttagctca acaacaggat tagagaatgc catatctgta
     4981 tattcttatg ccgctaaaag tggaatgcca ataatatggg ctaaagatga ggggtttgaa
     5041 gaacaaattg atttcttaaa aggtaaaaat cttaaaaaaa tatatgcatt aggtgattca
     5101 aaagaattta ttgcagagat tgattctaac ttaaaaaata tagagggcat aaagcaaata
     5161 aataagtcta gtacaaatgt tgatttaata aagaagtttt atgatgaaaa agatataaag
     5221 aaaatctata ctgctaagtt agactttggt agtagatctg atgtaaacga atatatttct
     5281 ttgggagtag tatcagcaaa agaaaatatg ccaatactga tttgtaatga taatttaagt
     5341 cgtgcacaag ataaattctt gaaagatagc aatataaatg atgtagttga agttgggtac
     5401 acagtaggtg attatagtct atttaagagc atatttaatc taacattctt atcttgtata
     5461 gtattaatac tattattact attgattaca tttagggcac ttagatatga gtcgaaatag
     5521 tttttgattt attaaggaag cttttcatag gttaaattta cttaattaga ataatgttta
     5581 taagcagttt taaaagttct acttttagag tttaaattag ataaggagaa agaagatgtc
     5641 aaaaacagct aaagcagcat tgtggattat ggcagccact atgttttcaa aagtattagg
     5701 tttccttaga gagcttgtac ttgcaaattt ctatggaaca ggtatgtatg cagatgtatt
     5761 tgtactaaca ttaaatatac ctggtttaat catagcagtc ataggttctg cagttgctac
     5821 aacttatata cctatgtact ttgagacaaa gaaaagactt ggagatgaag gtgccttaaa
     5881 atttactaat aatgttttaa atatatgcta tataatggct atagtaatag ctattattgg
     5941 gcttttattt acagagcaat ttgttacagt atttgcagca ggatttagaa acgaccctgc
     6001 aaagttccaa gcagcaatat tatttactaa aataatgatt tcaggagttt tattcctgag
     6061 tggaagtaaa atatttagtt cgtttttaca ggtgaatgac agttttgtaa ttcctgggct
     6121 tataggaatc ccatataata ttataataat agcagcaata gcactaagtg caggtaaaaa
     6181 tgtatggata ctaccagcag gagcactatt ggctatggcg agtcaactat tatttcaact
     6241 tccatttgca tttaagaagt cctataagta taagccatat ataaatttaa aggatgagtc
     6301 cataaaagaa ttagtcaatc tagtattgcc tatgttggta ggagtagctg ttggacagtt
     6361 aaatatattt gttgatagat tattggcaac tactttgggt gatggaaagc tgtctgctct
     6421 taactatgca aataggctaa atgagtttgt tatggcatta tttgttacat ctataataac
     6481 agttatatat cctaagttag caaaaatgtc tggtaaagat aataaagaag gatttataag
     6541 tactattgta aaatcgtcaa attgtattat attggtggtg cttcctatat ctataggagc
     6601 tattatatta gcagagcctc ttgttaggat attattccaa aggggtaaat ttgatgcgtt
     6661 atcaacagac cttacatcta tagcgcttag actatattca ctaggtttat tggcctgtgg
     6721 agtaagagat gttttatata gggcatttta ctctctttca gatacaaaaa cgccaatgat
     6781 aaatggaagt atagctttaa taataaatat agttcttaat ttaatattga taagaccatt
     6841 aggtcatgca ggtattgcca tatcaactag tacatccaat ataattacag ttatattatt
     6901 atttatatct cttaaaaaga aaaatggata ttttggagga gataaaatta taaagactgg
     6961 tctaaaaagt ttagtagcat ctggtgtgat ggcagtagtc actttattga tatataataa
     7021 tctatatgca tttataggtt caggaacaat taaagaaatt atatcagttg gtgcaggggt
     7081 actaggtgga gcttctgtat atacgatatt gatagtttta tttaaggtag aggaaatgga
     7141 tttagctttt gaatttttaa gaaaaggaaa acaaaaacta cttagaagat agattcttac
     7201 tttgaaattt taaattagag tcagaaagga tgaaaatatg gattataaga ataattatga
     7261 gatgtggtta aattctcctt attttgatga gcaaacgaaa aatgagctat taagcataaa
     7321 agatgatgaa aaagaaatac aagatagatt ctataaggat ttagagtttg gaacaggtgg
     7381 tttaagaggt ataataggag cgggaactaa tagaatcaat atttatactg ttagacgtgc
     7441 aacacttgga gttttaaatt atatcattaa gactcaagga gaggaaggta aacaaaaggg
     7501 aatagttata gcacatgata gtagatacat gtctagggag ttttgtatag aagtagcaaa
     7561 gactttaagt gcttatggtg taaaggctta tatttttgaa gaattaaagc ctacaccaga
     7621 actttctttt gctgttagat atttaaagtg tgctatggga atagtaataa cagcaagtca
     7681 taatcctaaa gaatacaatg gatataaagt atatgactca gatggtggtc aaatatgtat
     7741 agatgtggca aatgatataa tagcagaagt taataagatt gatgattaca gtgttattaa
     7801 aagtatagat tttgaagaag ctttatctaa aaatttaata actatattag ataatgaggt
     7861 agatgatgaa tttataaaag ctgttaagaa acaagtttta agacaaaata taatagatga
     7921 atatggaaaa aaattgaaaa taatatatac acctattcat ggtacaggaa ataagccagt
     7981 aagaagagtt ttaagtgaat gtggctttga aaatgtaatg gtagttaagg agcaagaatt
     8041 gccggattct aatttttcaa cagtaaagta tcctaatcct gaagaaaaat cagtgtttaa
     8101 tatagctatt gaaatggcaa agagtaatgg tactgatttg ataataggga cagaccctga
     8161 ttgtgataga gtaggtatcg tcgtaaaaga ttcaagtggt gaatatatag tcttaaatgg
     8221 aaatcaagtt ggttcactct tagttagata tatagtagaa agtttagtca aagaaaataa
     8281 acttcctaaa aacaatccta caataataaa aactatagtt acatcagaac taggtgctaa
     8341 aatagcaaag gcttataatg tagattgttt aaacacttta acaggattta aatttatagg
     8401 agaaaaaata aaagcgtttg aaaaaagcaa tgatagaagc tttataatgg gatatgagga
     8461 aagctatggt tacttaattg gaactcatgc tagagataaa gatggtgttg tatcttcact
     8521 tatgatatgt gaaatggcag catactattc ttcaaaaggt atgaacttgt atgaagcttt
     8581 gctagataca tataataaat ttggatacta caaagaggac ttaaagtcag tgactttaaa
     8641 aggtatagat ggaataaaaa aaataaaaga gatgatgctt tactttagaa gtgttaaaat
     8701 agataatgtg gctgatgtaa aagtagataa aattttagat tataaagaag gtgttgatga
     8761 tttacctaaa tcagatgtac ttaaattttt acttgaagat ggctcttgga tagctataag
     8821 accttcaggt acagaaccta aaattaaatt ttactttgga gcaaattcag acaatcaaga
     8881 agatgtagaa tttaaattga acaatttaat atcttatata ttaaatgtag tggattctat
     8941 ataagaggct aaggggagat aaataatgaa agtatataat gtcataatgg ctggaggagg
     9001 aggaactcgt ttctggccac ttagtagaca agaagtacct aaacagctta taaatttaag
     9061 tggagaagat gctttaataa acgaaactat aaacagaata gattctctag caaaaaaaga
     9121 tgatttattt atagtaacca atgagaaaca attggaagct ttaaaggata tagtaaaaga
     9181 taagtgtttg gataggaata tattaccaga gccatgtgcc agaaatacag cagcagctat
     9241 aggctttgca gcatttaata taatgaaaaa atatggtgat ggtgtcatgt gtgtatatcc
     9301 agctgaccat tatataaaag aggaaaaaga atttaaatct attttagaaa aagctatcta
     9361 tatagcagaa aataatgata aactagtaac tattgggata actcctacat ttccatcaac
     9421 aggatatgga tacataaatt ttaatagaga aaacacgata gaggatgttg catatgaggt
     9481 agtagagttt gtagaaaagc caaactatga aattgctaaa gaatatgtaa actctaaaaa
     9541 gtatgtatgg aatagtggca tgttcgtatg gaaggtatct aaaatattag aagattttaa
     9601 aagatacttg ccaaaagtat atgagaaact agaagagata agtaaatact taggaacaaa
     9661 agaagaagtg gaaaaaataa aagaaatata cccaactata caatcaatat ctatagatta
     9721 tggtattatg gaaagaagta atgatgttat agtagttcct ggtgattttg ggtggaatga
     9781 tgttggctct tgggattctc taggtgcaat atatccaact gatgatgaag gtaacatcaa
     9841 aagaggtgaa aatattacta tagatactaa aaattcaatt atatattcag atgataaatt
     9901 aatatcgaca ataggtatta gtgatttgat agtagtatct acgaatgatg cagttatggt
     9961 ttgtagaaaa gataaagctc aagatgtcaa aaaaatagta gagcagttaa aagaagaaga
    10021 tagacaagaa tatatgtagg aggtgttcat ttgattccta aaaaaataca ttatgtatgg
    10081 tttggtggtc caaaaggaaa tatagaaaat atatgtataa attcttggaa agaaaagctt
    10141 ccagaatatg agatagtaga gtggaatgaa aaaaactttg atatagagaa agaaataaaa
    10201 ggaaataagt ttttggaaga atgttataaa agaaagcttt gggcatttat atcagattat
    10261 acaagaataa gagtactcta tgaacaaggt ggagtttata tggatacaga tatgcaaata
    10321 ttaaaggaca taactcctct tttagaagat aataggctta tatgtggata tgaagatgat
    10381 ggagaatata tgaatgcagc tatactaggc gtagaaaaag gacatccttt ttttaaagat
    10441 ttactagagt actatgaaaa agagattctg acttcttcat tatttacaat accaaagatt
    10501 atgactcatc ttatggaaaa gaattataaa aagattgacc aaaataatta tgaagaagga
    10561 atacgtgttt acgataaaga atatttttat ccttttggat ttaaagaaga ctttacaccc
    10621 gagtgtatta cagaaaacac ttatggtata cattggtggg gaaaaagttg ggctaaaaag
    10681 agaaattatt tcttagaaag caaacactta acaggtgtaa ataagatatg gaagtgttgt
    10741 aaaatatttg caagtaatac tttacgaagc taaaagggag aaagtaaaat gaagacttta
    10801 aacgatgata gaaatattat gattagaaaa tatctttata tatttgtttt gtttatcata
    10861 ttacttagaa agtatatgat atcatttatt gacccaaata tggatatagg gatgattaaa
    10921 tcagcgttat tttattcttc aataggaata ctaatgctac tttttttatt tgataagaga
    10981 aaaagtataa cagaaatggt actagttgga gtttgtgtct tactttattt attaaatagg
    11041 gaaggagcta tattattaat cgttttatta gctgtatcag ctaagcaaat agatgataaa
    11101 tttatagtta aaaattattt aataatatcg gcatgttttt taatggtagc tatattatta
    11161 tttaatttat ttccttcatt aatatttaat caggaagtcc actatagata tatagaaaag
    11221 atagatatgc ttgttacaag aatggatttt ggacttggta atccaaatag tgtattttat
    11281 catatggtta ctatatatgc agcatatata ttcttaagat ataaagacta taataaatgg
    11341 gatagaataa ttttatttgg ttctgcattt tttgtttatc aaaccacata tagtagaact
    11401 ggatttttta ctatattagc aggattaata tttgtagaaa taataagatg gatagacata
    11461 aaaaaaataa aaggactacc aatgcttcta aagacattac caataatact tactttattt
    11521 agtgtaatta ttggtacagt atttgataag agtactttat taaatagatt attagcaagt
    11581 aggcctaagt tctggaatgt atacttggct gaaaagggga actttctaaa accttttgga
    11641 aattcatata gtcctgctat aaaagcaact aatccgttag atagttctta tgtatatata
    11701 attagtgtac taggagtcgt tgcttgtata ttatttatgt atttgatgta taaaggaata
    11761 gaaagcttta tagaaaaaga taaaaaagca tatttagtag ctttatttat atttttacta
    11821 tactcttttg cagaaaattt attattagaa gcttcattta gttttgcagt agtattacta
    11881 ataaaagaag tgatattaaa tgataaaagg gaaatagatt tgtggaagat gaaaagtagg
    11941 aggtagtaat gttaatatca ttaataatgc caacattaaa tagatatgat gatatatact
    12001 tacttatgga cagcttagaa aatcaaactt acaaaaactt tgaacttata gtagtagacc
    12061 aaaatgataa tagcaaagtt aaagaaatag ttgataaata cattgataag ttagatataa
    12121 agtatataaa aagctctaaa aaaggattga gttataaccg aaatgtaggc atagacaatg
    12181 ctgtagggca aattataggt ttccctgatg atgattgtgt ctacgaaaat gatacattag
    12241 aaaaagttat aaatttcttt aataaaaata aagattataa aatatacagt tgcaaaacta
    12301 tggatgcaaa taaaattgat gcattcaaga aaatgtatga tggaacttgt gatatcacaa
    12361 gttccaacgt tttggatact ataacatcca taactttttt tattgatttt gaaggtaaag
    12421 actatactag atttgatgaa aaattgggtg ttggtggaga gtttggtgct ggtgaagaga
    12481 tagattatgt tttaaactta ttgagtttag gttttaaagg aaaatacttt ggagatgata
    12541 taatttacca tcctgcaaaa aaacattcta aatctaaaga aaagtatcaa aaagattata
    12601 attatggaag aggttttgga gcactttgca aaaaggaaat agtttataga aaaaattata
    12661 agtttgtaaa ggttatggta tcaaaacttg taagaaatat aggaggtctt atattgagtt
    12721 ccaatagaga ttatcatagt gccactataa aaggaagaat taatggattt agacagtaca
    12781 agttataatg aggatggtgt ttatggacaa ggttaaattt ttaataaata tgcttttggc
    12841 tttttttata tatccattta ataagcatag gtttaaaggt agaaatatct ggcttatagg
    12901 tggacattca ggggatatat acaatgataa ttcaaagttt ttttatgagt acatgttgaa
    12961 ggaacataat gatgtagaaa cttattgggt agtaaataaa gacagtaaag tatttgataa
    13021 aattccaggc aaaaaattaa taagaggaag tgtagaaaac tatctgtatt attacaattc
    13081 aaaagctata gtattttcac atgcaccatc agcagatata gcaccataca attttgctgt
    13141 acctgttctt aattattttc ataaaaaaac aataaaagtg tttttaaatc atgggactat
    13201 aagttttaaa aagagaaaac ctatgaacaa aaagttcaaa aatattatag ataacttata
    13261 taaaagctat aatatagtta cagcaagttc tgaatttgaa agaaatgtta tggtaaatga
    13321 ttggggaatg ttagatgact cagtttatat aattggaaat gccagatatg acaatttacc
    13381 aacaaatgaa gttgcacaaa ctcgagatat attatatact cctacatgga gagattggat
    13441 aaaatttagt tcaggaaaat ttacagatac agattatttt aaaaatataa tgaatttttt
    13501 aaatgatgat aaattaaata aaatattaga tgagaaagat ataaatgtaa aaatttatat
    13561 gcatcatctt atgcatgagt ttatagatga tataaaagaa aatataacag gaaaacgaat
    13621 tgtgttttta gataaagggg taactctagc aaatgaaatc agaaaatcag ctgcaaatat
    13681 aactgattat tcaagtgtag ccatagactt tttgtatatg aatagaccta tcttgtttta
    13741 ccaatttgat ttggatgaat atatggaaaa agttgattca tatatagatt taaaaagtga
    13801 aatgtttggg tctttggctt acaataatga tgaagccgtg aataaactta ttgatattat
    13861 tgaaaataat tttgaggtta tggataacca aaaaaatgaa agaaataagt ttttcagata
    13921 taatgataat aagaactgca agaggatata tgattgtgta ttaagtaaaa ttaaataaat
    13981 attttataaa tgatgaggaa ggtaatatga agaagaattt agtatctata attactccca
    14041 tgtataattc tgaaaaattt attgaagcaa ccataaaatc agtattaaac caaacttatc
    14101 aagaatggga aatgttaatt attgatgatt gctcaacaga taatagtcct aatatagtca
    14161 aatcttatat gcaacaggat agtagaataa aatgtataaa gactgagact aataagggtg
    14221 tctctaatgc tagaaactta gcactaagta aggcaacagg acaatttata gcttttttag
    14281 acagtgatga ccaatggaat agtagtaaat tagaaaaaca agtaaacttt atgttagaaa
    14341 atgactatgt aatttcattt acttcatatg aactgatgga tgaaaatgat aaaaaattaa
    14401 acaaagtaat aaaagttcca cctaatgtag attatagaag attattaaaa ggaaatatct
    14461 taggatgtct taccgttgtg attgataagt ctaaattgga ttttgaaata agaatgagtg
    14521 gtgtaagaca tgaagattat gttttatggt tatcgatatt gaaaaaaggg catattgctc
    14581 atggaataaa tgaagttcta gcgttatata gaaaatctag caattcccta agtggtaata
    14641 aaataaaagc tgctatgtgg acttggaata tatatagaaa tatagaaaaa atacctttat
    14701 acaaagctat atactacttt attaattatg gaataaacgg aatcaaaaaa agttagacag
    14761 agaggaagat ttaattaatg tataaatata aatttactgt atttactcca acgtataata
    14821 gagctcattt acttgaaaat ttgtacaatg atttaaaagc tcaaacttat gatttcaatg
    14881 attttgaatg gttgattgtt gatgatggaa gtagcgacag tactaaagag ttagttgaaa
    14941 aatttatatc agaaaataaa ttaaatataa gatatatata caaagaaaat ggaggaaaac
    15001 atactgcaat aaatctaggt gtaaaaaatg cagatggaga gctatttttc attgtagata
    15061 gtgatgatgg tttaattaaa gattctcttg aaattgctaa tgatgagtgg gattctttag
    15121 agaataaaga aggatttagt ggagttgttg gactttgtgt atatccaagt ggagatttga
    15181 tagggacaga aatgcctgaa gataagaaaa tatgtcactt tgcagaccta tattttaagt
    15241 atggcgttaa gggagataaa agcatagtat ttgtaactga tgagcttata aaatttcctt
    15301 ttccagaaag aaaagaagtg agatttttac cagaaagtgt agtatggaat gaaatgtcta
    15361 aatactataa agttatatgt gtaaataaac ctatgataat aagagattat ttggatgatg
    15421 gtttgactaa gaatatatta agtaaaaatg cattaaaagg tagagcatta gagtttttat
    15481 atttaataaa tcaaaataca tatccattaa gtagatatcc atatatgtgg ataaaaaatt
    15541 acataaattt agccagatac tcattgttgt ctgatagtca ttattttggt gaattaagaa
    15601 aagtatctga taaattgctg tatttagcat tattcccatt aggatattac aaatatattg
    15661 gacaaagaaa attagtatct aagtaaactt gttggtatag tactgctagt tgctatataa
    15721 ggagagtaaa aatatgaatg agcctttagt atcgataatt actcctgttt ataattcaga
    15781 agagttttta tcagagacga taaagtcaat ccaaaatcaa acttataaaa attggcagct
    15841 tttattagtg gatgattgtt ctaaggacaa ttcaagtgaa attataaaaa gttttcgaaa
    15901 agaagatgct agaataaaat atataaagct tgaaaaaaat tctggagctg cagtatctag
    15961 aaatgttggt attaagaatg cggaaggtcg atttattgca tttgtagata gtgatgattt
    16021 atgggatagt agaaaattag agattcagat agaatatatg ttaaaagaaa atataggttt
    16081 ttcttttaca tcttatagat atatgagaca agatgggtca aaaacgaata aggttgcaag
    16141 agctcctaag aaaattgact atgaaggatt acttagaaat actataatag gatgttctac
    16201 agtggtcatt gataaggaga ttgtaggaga gttcagcatg cctttggtaa gaaggggaca
    16261 agatactgca acctggttac agttacttaa aaaggaaaaa tatgcatatg ggattcagga
    16321 agatttagtg aattacagat tagttggaaa ttctatatca agtaacaaga taaaagcatt
    16381 aaaacgaact tggaatactt atagaaatgt tgaaaattta agtttaccaa agagtctata
    16441 tgtattttgt ttctatgttt ttaatgccat taaaaaaaga gtataagttt tatatacttg
    16501 gaggtaaaaa aatgaaaata gctatagctg ggacgggata tgtaggtttg gttacaggtg
    16561 tttgtctagc agaagtaggc aatgaaaatg tagtctgtgt tgatgtagat gaacaaaaag
    16621 ttaagtcaat gaaacttgga atagcaccta tatatgaaga aggcctagaa gaacttatgg
    16681 ttaaaaatta caatagagga agaatagatt acactacaga ctataaaaaa gcttatgaag
    16741 atgtagatgt aatactaata gctgtaggta caccagaaag aagtgatggt tcagctaact
    16801 tagattatat aaaggcagtt gcaaagcaaa tagctgaaac tgtgacaaaa gatactctag
    16861 tagtaataaa atctacagtg ccaattggta caaatgaaga gatagaagat tatataaata
    16921 gaaacttaaa gaatgatgta aaagtggagc ttgcatctaa cccagagttt ttatcacaag
    16981 gtacagcagt aaaagatact ttgcatgctt caagaatcgt tataggtgta gaaagtgaat
    17041 gggctagaga tatattacaa caaatttatg agccttttaa tcaacctata atagttacaa
    17101 atagaaatag tgctgaaatg ataaagtatg catcaaataa cttcttggct cttaaaatat
    17161 catttatgaa tgatattgca aatctttgtg aaatagcagg agctaatata gaagatgtag
    17221 ctttagggat gagttatgat gatagaatag gcagtaaatt tttaaaggct ggaataggat
    17281 atggaggctc ttgctttcca aaagacacaa aagctcttca ctggttatct gaacataatg
    17341 gttatgaatt aaaaacagta aaagcagcaa tagaagttaa tgaaagtcaa aaacttaaat
    17401 tggttagaaa agcttctaaa ctagtagaga gttttcaaga tatgaaggtt gccgttttgg
    17461 gtttgacttt taagccagat actgatgata taagagaagc gccatctata ccaaatataa
    17521 aatatttgct cgaaagaggg gcaagagtta ctgtatacga cccaattggt gtaaataata
    17581 ctaagaaaat atttggtgat tctatacttt atgcggattc tgtagatgat gcgatagaag
    17641 aggcagagat atgttttata atgacagaat ggaaagatgt attaaattat gatatacata
    17701 aatataagga aaagatgaaa aatcctttag ttcttgatgg aagaaattgt tataaagtta
    17761 aagatatgga agagttagga attgagtatt attcgatagg aaggtaaata ttatgaagac
    17821 tgtttgttat ttagctgatg ggtcaaatcc tcatacttta aaatggtgta gtttttttaa
    17881 aaataaagga tatgacattc atgtaatctc tttaaatgga ggccacatgg aaggaataaa
    17941 aatttataac tttggttcta atgtagaaga actaaaaaat gaaaatattt ctaaaaaaac
    18001 aggatactta ggttcagtaa agcagataaa gaagctagta aatgaaataa agccagatat
    18061 attgcatgca cagtatgcaa gtagttatgg atttatagga agtctacttg ggtatcatcc
    18121 atatattatt tctgtttggg gtactgatat ttatgatttc cctaataatg gttttataca
    18181 aaaacaaatt ataaaacata actttagaaa agcagactat atcttttcaa atagtagaga
    18241 tatggctaaa gaggctaata aatatacagc taaacatgta gatgtaacct tttttggagt
    18301 agatatggat aggtttaagc ctatggaagt agaaaaagaa gatgcttttg taataggaat
    18361 tataaagagt ttagaaaaaa aatacggaat agaatattta atacaagcat ttaagatgct
    18421 taaagatgaa tataaagata agaaaataat tcttaaaata ggtggttctg gaagtcagat
    18481 ggacaaccta ataaatttaa ctaaagaatt gggtatagaa gatgatgttc agtttttggg
    18541 aagaatatct ccagaaaatg taagcaagac ttttaattct tttgatgtaa ctgtgtttcc
    18601 ttctttaaga gagggatttg gagtggcagc tatagaatca gaagcgtgtg aagtacctgt
    18661 tatagttaca aatgtaggag gtcatccaga atctgtatgg gaaaatgaaa cgggcttaat
    18721 tgtagaacca aaacaaccag aagaaataaa aaatgctata ataaaactaa tggaaaatga
    18781 tgaacttaga cttagtatgg gtaagaaggg tcgtcaattt gttagagaaa attatgaagt
    18841 taatttaaac tttaatgata tagaaaaaat ttatgattct atatttgata aatataaaaa
    18901 ataaaatgaa aaatacagca aaagatagat gttgttggct atgcttaggt gcgtagcttt
    18961 tgctgtttaa aggagggctt ctagttatgc gagaaagtat agatttagag aaagaaagct
    19021 tttttaatag gctttttaaa aaatataaat ttagacattt aacactatta attctagata
    19081 tttgtagcgt actagtagcg tttcgaatgt ctttaagctt gacaggtaat ttaaatgtat
    19141 ttaccactaa tgatataata atttcagctt gtatatatat agtacttcat atagtatcat
    19201 ttagattgtt taagtgttac aacacactat ggagatatgc aggagaagaa gaaatcatat
    19261 ctatttttat ggcaacttta gcttatttaa ttccagttta tataataaat aaattattag
    19321 ggtttgatta tcctataata ttttatgtac taaatacaat atttattata atgtttacaa
    19381 gtggcgcaag aatagcctat agagctataa gaatagtcat gaataagaca tattctagag
    19441 gcaagatttc aaatatcctt ataattggtg caggagatgc aggagaaatg gttatacagg
    19501 aactaaaaag aaatccagaa cttaagaaag tagcagtggc tataattgat gatgataaaa
    19561 ataaaattgg aagaataatc cataatgtaa aaattgtagg gacaacttca aatataaaag
    19621 aggtagttga aaagtacaat gtagatgaga taatcttttc aatagcaaat atagagaaac
    19681 gtagaaaaaa agagattatt gatatatgta aaaacactaa ctgtaaaata aagacaatac
    19741 caggaattta cgaaattata gatggaaaag ttgatataaa acagattaga gaagtagata
    19801 tagaagactt gttgggcaga gagcccataa aaacaaattt aagagagata agtaattata
    19861 ttgagggaaa agtaatccta ataactggag gaggaggctc aattggttca gagctttgca
    19921 gacagattgc aggatttaat cctaaagagc ttattatagt tgataattat gaaaacaatg
    19981 cttactcaat acaacaagaa ttacttagaa aatataaaaa taaattagac ttaaagaccg
    20041 taatagcatc aataagagaa gaaaaaagaa tggatgaaat atttaataaa tataagccag
    20101 aagtagtatt tcatgcagca gcacataaac atgttcccct tatggagtcg agccctggcg
    20161 aagctataaa gaataatata tttggaactt taaatatagc tggattatct tcaaaatata
    20221 gagctaagaa atttgtcctt atatctacgg ataaggctgt aaaccctact aatataatgg
    20281 gagctactaa aagagctgct gaaatgatta tacagactat gaatgctgaa agccagactg
    20341 aatttgttgc tgttagattt ggaaatgtac ttggtagtaa tggtagtgta attccacttt
    20401 ttaagaagca aattgaagat ggtggaccag tgacagttac tcatccagat atcattagat
    20461 attttatgac aataccagaa gcagtaggtt tagttataca agctggagct atggctaaag
    20521 gtggagaaat atttgtgctt gatatgggag agcctgtaag aatattggat ttagcaaaaa
    20581 atttaattaa attcagtgga tttgagccag atgttgatat aaaaattgaa ttttctggtc
    20641 ttagaccagg agaaaagttg tatgaagaac tacttatgtc agaagaaggt ctattggata
    20701 cagagcataa aaagattttt attggtagac caatagatgt tgatagagaa aaaataacaa
    20761 aatacttaaa acttttaaga gaaattacta acaatgaaga ggttgaaaaa atagatggta
    20821 ttatgaggga attagtacca acttatatta aaccagaaga tgctaatatc aaagaaattg
    20881 caacaactag agaaaagtag aatatatttc taatttgtaa ccagggggag caaaaagaaa
    20941 caaatatgtg ctaaaaagaa acaaaagatt acaaaaagat tacaaaaaag gttgcaattt
    21001 attttgatgt aagatagaat gttaattagg aattggaaac gaaggaacaa aatgttaaat
    21061 tttcgaaagg attaggtatt tgagattatg atgaatatta aaaataaaaa acatatattg
    21121 aaaaaattta tagctatggt gttaatagca ggagttgtaa cagtagaagc aggagcaata
    21181 acagctagtg cagcagagct aactaattca cctatgagtg caacagtaga ccagtgtgat
    21241 tttttaaatg taagaagtgg agctagtgca aatgatgcag tagtaggaaa gataaacaca
    21301 ggagacaaag ttgaagtctt agaattacat tcaaatggat ggattaaaat aaaaactgtc
    21361 gacaatgtta caggatgggt aaatggagat tatttaacta ttcaaggtgg aaatgtagat
    21421 gcaaaggtac aaaatgtttt aaacttagca tttaagcaac aaggtaagcc atatagatgg
    21481 ggagcaactg gaccaaattc ttttgattgt tctgggttca cttcttatgt ctataaaaat
    21541 ggagcaggag ttaatttacc aagagtatca agatcacaag caacagtagg aaagaaagta
    21601 tcaagagcag aattaaaacc aggagattta gttttctttg gttcaggcgg ttcaataaat
    21661 catgttggat tgtatgtagg aaattctaaa ttcatacatt caccacaaac aggagatgta
    21721 gtaaaagtta ctagcatggc tccaggaact aattatgcaa gaagacttat aactgctaca
    21781 cgtgttttac aataataata gatattgatt tacataggat tgaatattaa cattaagtat
    21841 ataattacat ttaatgtttt acatagagat ttaagagttt agtaatatta aaatagtgat
    21901 gaacgttata aaaacagcaa agaagataaa tttctttgct gtttttttat aataaattat
    21961 ttcatattat aatcaaatat aaaatataaa ttttaactat attattcaat atttgacaga
    22021 taatatgaat tttagatata cttgataaga agtatatttt agaaaaaata aatttaaaaa
    22081 attaaggggg atgaaatgaa aaaaataagt atattagtat tatcattgat aatgacactt
    22141 actatgtgca gtgtaagttc atttgctgat tcgtcaaatg ataaggaaat gagagctgct
    22201 tggatatcta cagtttataa tttagactgg cctaaaacta agaacaatga agccaagcaa
    22261 aaaaaggaat acacagattt attggacaaa ttaaaaagtg taggaataaa tacagcagtg
    22321 gttcaagtaa gaccaaaatc agatgcttta tataaatcaa atataaaccc atggtctgaa
    22381 tatcttacag gtacacaagg taaagaccct ggatatgacc cattaccatt tttaatagaa
    22441 gaagcacata aaagaggaat ggaatttcat gcgtggttta atccatatag aataactatg
    22501 gcagatgaga gcatagataa attgccagca aacaatccag caaagaaaaa tccaagttgg
    22561 gtagttaaac atggaaataa gtattactat gacccaggtt taccagaagt tagaaagtac
    22621 atagtggata gtatagcaga agtagttcaa aattacgata tagatggagt tcattttgat
    22681 gattatttct atccaggagt aagtttcaat gatactgcta cataccaaaa atatggaaat
    22741 ggacaaaata aagataattg gagaagagaa aatgtaaata ctctacttag agatgtaaaa
    22801 gcgtctataa agagtataaa gcctaatgtt gtatttggtg taagcccagc aggtatatgg
    22861 agaaataaga gtagtgaccc aacaggttca gatacatcag gcaatgaaag ttatgtaggt
    22921 acatatgcag atacaagagc atggataaaa caaggattga ttgactatgt agttccacaa
    22981 ctttactggc caattggtct taaagcagca gattattcta agttagtgtc atggtgggct
    23041 aatgaagtaa aaggaacaaa cgtagattta tacataggac aaggtatata taagcaaggt
    23101 caatcaagtt atggtggaca aaatatagca aaagaaatag tgcaacaagt aacattaaat
    23161 agaaagtata gtgaaataaa aggtagtatg tatttctcag ctaaagatat agcaaatagt
    23221 agctctatac aaaaggattt aaagagctta tattctagta gcgaagaacc agttactcca
    23281 ccatctaatg ttaaagtgga gaaattaaga ggcgacgaaa ggtatgatac agcagtagca
    23341 ataagtaaaa aaggctggcc tacaaattca gatacagtag tattagtaaa tggatactct
    23401 atagttgatg gtataacatc aacaccactt gctacatcaa atgatgctcc tatactattg
    23461 gtaaataagg ataatatacc tacatctact aagaatgagt taaaaagact taatccaagt
    23521 aaagttattt taattggagg aaatagctct attggagata aagttgagag tgagataaaa
    23581 gatacactgt caaatgtatc aataaataga gttggtggtt cagatagata tagtacatct
    23641 ttaatgatag caaaagaatt agtaaaaaat aatccagtag aaaaactata tataacaagt
    23701 ggtacaggtg aagcagactc tctttctata gcttcaaaag caggagaaga aaagcaacca
    23761 atagtactag tatcaaaaga taatgtaagt gatgaagttt acaattggat atcagactta
    23821 aaagtaaaag atgcttattt tataggtggt aatctaagta tatctgattc agtaataaat
    23881 aagttggata aagtcattac aaatgatgtt tctaaaaata gaatagctgg agaaaataga
    23941 gaagagacaa atggaaaagt aatacaaaaa ttctatccaa atgctgaata ctcatcaatg
    24001 tttgtaagta aaagtaatca attggtagat gcattaacat caggtccttt agcagctaaa
    24061 ttaaaatcac cagtagttat gcttggaaat agcgtaacat ctgctcagaa aactgcttta
    24121 gagcctaaga aaactaatct agtatataca gctggagatg gaataaatca aaatacatta
    24181 aatgctttct taaacttggt taattaataa aaaagttaat ttaggataaa atttctttta
    24241 tagatgtaga ttaaaatgaa tattattaag aaaaattaga aaataaaaaa gaactatatg
    24301 cattttgtaa tcaagtgtac acagtagctt gaaaataatt atgcgatata gttctttttt
    24361 actaattaat cttatacttt gtaataaact ttatattaaa ttttttattg tttaaactct
    24421 atgtcattaa atataaaatc atgtaatatg tctaaagtat ctgggtcaaa tctaagaacc
    24481 catccagctt ttccatatat accacctgta ctatgaattt catcatcaat aggaaattca
    24541 aattgcttaa tatttaagtc ccccattttt aaaacttgag ctcctaaaga aagtattgca
    24601 ttaggcttca tgttagtttt aacatatgga agaactgcat ctaataatcc tggatattta
    24661 gtaacaggta aagtttctgt tttcttgatt atagcttgta taatctgtct ctgtcttcca
    24721 tctctagcaa aggctgtatc attgtgtctg atacgtgcaa aagatagtgc ctgatatcca
    24781 tttagagtct gctttccagc gtttctaatg tattgtatgc tacctttatc atcactttta
    24841 ttccatttat aagtctcagg aataaattta tttagttctc gtatttctcc tttttcaatg
    24901 gtaacttcaa cgccaccaat agcatcaatt atatacataa atgattcgaa attaacacaa
    24961 gcgtaatttt gaatatcaat attaaaattc tcttcaattg tttgtattaa taaatcagct
    25021 tgtccataag cataagcatg agtaagtttt tgtttaccat gaccagggat gtctacatag
    25081 ctatcacgag ctaatgatgt aagtttgata tcattgtgct tgttatcaat agtaagtatc
    25141 atcatagcat ctgaccttga agaatcttca tttggtctag catctgttcc cataagtagt
    25201 atattaatta tcccatcttc atttttatgg tcattgttat ctaataaatc actacttatg
    25261 ctcgagtcat gaatagcaga taatttataa taaaaatatc catagacgct tatagggaat
    25321 attactacca agaaagccag tagtataaca aatttcttta attttgacaa aagtacccct
    25381 tctttcttct ttttcctgta tattttttaa tagcatacac ttaattatat taaagtattg
    25441 aaatatattc aatacttgtt tcagttacaa aaaaaagaca atgatataat ttataagaaa
    25501 atttatcgac ttatatatac aattaaaagt atatataata tattttttaa aagtaattta
    25561 tattttaaaa taaaaacaca ccccgtaaat gcaaaataat ttgcatttaa gaggtatgta
    25621 tttatcatca aattattagt ttgttttgaa atttctaatc ttcaaccata atgtctttaa
    25681 atatgaaatc atgcattgca tctagttcat attcctcaaa aggtattaca tagcctgcat
    25741 tacctatttt tctaccattt tcagtagaaa gaggaaattc catagatttc aaatctaaat
    25801 ttcctatact taaaagttct tttcctaaag atattatttc attaggattc atattagttt
    25861 ttacatacgg aagtattgta ttaactagac tagggtattt agtgactggt aaatccttaa
    25921 caccatttat taaactctgt atgacacttc tttgtcttct atctctttca tctgtactat
    25981 cgttttttct tatacgagaa aatgcaagtg tttgatatcc atttaatttt tgctcaccag
    26041 aatttcttat atatttaaga gaacctttat tgtcattttt attccattta taagtttcag
    26101 gtataaattt atttaattct cttatttcac tcttatcaac agtaacagta actccaccta
    26161 gagcatcaac tatatccata aaagagaaaa agtcaactac agcgtagttt tgtatatcaa
    26221 gttggaagtt ttcttctata gtttctatta ataaattagc ttgtccatat acataggcat
    26281 gtgttaattt ttgttctcca cgtcctggta tattaacata agtatctcta ttcaatgaag
    26341 ttagtttaag acttttattt ttattatcaa tagtaagtat catcatggca tcagaacgag
    26401 aaacttcttc tcctggtctt gcatctgtac ctaacaataa tatgtttgtg atattttctt
    26461 cgtttttata gtcattacta ctaagcgcct ctgtatttat actatcatct tgtatagttt
    26521 ttaatttagt aaaaacatat ccatatatac taacaggaaa tgctataact aaaagggcta
    26581 gaaatataac gaactttttg agtcctgaca ataatatccc ctactttcta aattttttaa
    26641 ttacacatag ttaattatat taaaaattta ctacataagc aataaaaaac acaataaatt
    26701 agcataagtt tgtcataata taattatttt catagtattt tatagaagaa agtgtgtagt
    26761 tattattaat tttattggaa tataattgta tgaagaatta aatttttata aactggtgtg
    26821 tatattgaaa aaaatatttt cctgaatgta gttatagcat cttattgaaa ggtgtaaatt
    26881 gtaatttaaa atttttagtg tataataaga attgaaaatt taaactgata aaaattaata
    26941 aaaaataatt atagattaat aatttggggg aggaaatgta tgataaaatt aatagcaaca
    27001 gatttagatg gaactttgtt agatgaaaag agcgaaataa atccagagtt ttataaagta
    27061 tttaaaaaat taagagagag aggaataatg ttttctgctg ctagtggcag gcagtatcaa
    27121 aatcttataa aaaaatttga agctataaaa gatgatatga tgtttatctc agaaaatgga
    27181 acattggtag tatataaagg aaaagagata ttatcaaatc ctttggataa agaattagta
    27241 aatgaaatta tagagacaac aagaagcata gaaggtaaga aaatcgtaat gagtggtaaa
    27301 aaatacgctt atatagaaag taaagatgaa gcctttatag atgaagttag tacttactat
    27361 gcaaagttta aagttgtaga tgacttgact aaggtagaag gtgatatatt aaagatagcc
    27421 atatttgact ttaaaggagc agagcataat aatagcatct actttgaaaa gttttctgat
    27481 agagctcaag tatgtatttc tggagtagaa tggcttgatt taactgcaaa aggtgcaaat
    27541 aaaggaagtg ctataaaaaa agtacaaaaa atgttagata taaaatatga agaaactatg
    27601 gtatttggcg accaattaaa tgatgttgaa atgatgaaaa gtgcttatca tagttatgcc
    27661 atggaaaatg caaatgagta tttgaaagaa attgctagat tcagagcaaa aagaaatact
    27721 gagaatggcg ttgtagataa gataaaagag gtcatgaaaa taggatagtc atatgtatgg
    27781 atgaaaagat tattttgaga aactaaaaaa atatcaatgt aattttgata aataaattta
    27841 tttatgtgaa aattacattg atatttaata aacatcaata agaatcaata agagatattt
    27901 atttttttat tatactcagt atcttgtttt ctatttgagg tgcattttca gcagaaatta
    27961 aataaacata tctacctgcc ttctcaatta tagtattatt tgctatttca gaattgattt
    28021 tttctccatg tccttctcca aaaggtgctt gtatcatatc ttttttataa ttttctagag
    28081 ttttatagat tttatctacc tcgtcagttt ttatgacaat aatatcttct agagataatt
    28141 tttcagaaga attaattaca aaaccttgaa tgatgtattc ctccacatca ttaaaatagt
    28201 taaagtcctt tgcatcaaag atttttgatt cttttataag taaatcagaa gattttaatg
    28261 cttttttaat ttttgatgta gaaacgtttt tataatcacc acttgaagaa caactaacca
    28321 ttcctaatga taagattatc gaaaaaaaca atattaaaat ttttttcatg ataaacctcc
    28381 aagtttttta aactctgtct taaattagtt tactacataa ttatataaaa ataaatgtat
    28441 ttaatgttac agttttgatt aataatgtaa tattttgata ataataccta ttaagaggtg
    28501 attagaaatg agaataccaa aacatgtagg aattatacca gatggaaaca gaagatgggc
    28561 aagtcaaaat ggtatgacaa aagataaagg atatgaaagt ggaatagacc ctggactaga
    28621 gtcttataga ttatgtagag atttgggctg tgaagaggta actttttatg gatttacaac
    28681 tgataataca agacgtccgt cagtgcagac aaaagctttt acaaaagcat gtattgatgc
    28741 tgcaaatata ttaatagatg aaggtgcagc tttactagtt gttggaaata caaaatctcc
    28801 aatgtttcca gaagaattat taccatatac taatagaatt aaagctaaag aaggtcaaat
    28861 aaaagcaaat cttctggtaa attacgggtg gtattgggac ttaaataata tatcaggagt
    28921 agaaaatgta aataaaaata atatacagga ttacatacag tcatttgatg tatccagact
    28981 agacttgata atcagatggg ggggaagacg gagattaagt ggtttccttc ctgtgcaatc
    29041 aatttatgca gacttttatg ttatagataa ttattggcca gattttaaga gtgacgatat
    29101 aaaggatgca tttagttggt atgatacaca agatgtcact ttaggtggat aattaaaaat
    29161 attctgattt aaggttgtag agtgttttaa attattgaaa aaaaaacttc ttgatggtaa
    29221 aataacacta ataatatgta aaagtgtaaa aatttatacc aagaagggtg aagaaatgtc
    29281 aaaaggcaat aataacaata attcaagaaa caaatctaaa aagaccagtc atctgaatag
    29341 aaaaaagcga aaattaaata agaaaaagtt agcagtgttg atttgcttta cagttttatt
    29401 tttatttatt gcatttaagg ttactcaagg tgtagtagct ttggtaaaaa gtatggataa
    29461 aggtaataaa actagccaac aacaaaatgt taactcagaa caatttgacc ttggtaatga
    29521 agaggaaaat aaaaataaaa aatatactgt atttatagat ccaggtcatg gtggcaatga
    29581 taaaggaact gaaagcaaga ctagtcatag atatgaaaaa gacctaaatc tacagattgc
    29641 aaaaaaatta gcaaataaat tatctaaaca aaaggatatt caagtggtag tatctagaac
    29701 tgatgataca tatatatcat taaaggatag agctatctta gcaaataata gttctgctga
    29761 tgtgcttgtt tctattcatt taaatgcaga gaaaaatggg aatacagcaa caggtataga
    29821 aacttggtat aggaataaag ctacagacgg ttcaaaagaa cttgcacaga cagtacaaag
    29881 tactatagta tcctatgtta aagttagaga taggggaata gttgaaaata actttgaagt
    29941 acttagagaa tcaaacatgc ctgctatatt gatagagtgt ggatttttaa caacaccttc
    30001 agatgaacaa aaaataataa atgaaaaata tcaagaacaa cttgcagaag gaattgttca
    30061 aggtgtgctt tcatacttag attctaaagg gaataagtaa gttaacttta ttttaatttg
    30121 aggataaatt taaaaattta atgtgtagat tgtattaact ttatttcgta atgttggaaa
    30181 cgttgtttga atttagaaac tatgaaatta gaaattattt tatctaatct aatttcatag
    30241 ttttttttgt aaaaaattaa taattataaa aatttaaaaa aataaattta gcatagtgta
    30301 atgtggttat ttatatgcga ttttaacttg c
//
LOCUS       DAFNNB010000030        30309 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0030, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000030 DAFNNB010000000
VERSION     DAFNNB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 30309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30309
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0030"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            47..253
                     /locus_tag="L3M60_001197"
     CDS             47..253
                     /locus_tag="L3M60_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225884.1"
                     /translation="MSKYSSLWEYVKKNNSQSFKLTFEEIKDIAGVEIDHSFLKYKKE
                     LNEYGYQVGKISLKEKTVIFDKIG"
     gene            complement(559..1302)
                     /locus_tag="L3M60_001198"
     CDS             complement(559..1302)
                     /locus_tag="L3M60_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBN6225885.1"
                     /translation="MNFLSDLHTHSIVSGHGYSTLLENINYCKENGIKILGTSEHGPK
                     MPGSPHRWYFHNIKNIPRIINNIIILRGCEANILDTKGNIDLEPFVIPRLDYLILSFH
                     EAVFSPNTLENNTKALINAINKHDNIEILGHLGNPNYPIDYELIIKLAIEKNILIEIN
                     NCSIKGVSRNGSSDNCKYIATLCKKYGAKIILTSDAHICFDIGNYEYSENILKEINFP
                     DELIMNHPKKLINHFHQKGKLLDVDYRNI"
     gene            1468..4035
                     /locus_tag="L3M60_001199"
     CDS             1468..4035
                     /locus_tag="L3M60_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6225886.1"
                     /translation="MTQRKLSRKEKVLICLKRLTEEHLNFNNGIRFGFDAEYIATEVG
                     INRSNASKELNQLIKESIIIKIKGKPVQYLYKESIESILGKKLNKGIFSNINSINEYS
                     EDDSIKDEYILEGIKKENNIFNLVGCDGSLKSQIEQAKAAVIYPPKGLSTLIIGPTGV
                     GKSIFAEYMYKYSLNIKEDSDNAPFIIFNCADYSDNQQLLLAQLFGYVKGAFTGADKD
                     KYGIVHEANNGILFLDEVHRLSAEGQEMLFLLMDKGIYRRLGEANKVHECRVMIIAAT
                     TEDPETAMLETFLRRIPVTIKIPSLEERGFQERMKLICYFFKEESIRIGVKLKVSKEV
                     IKAFILYKCKGNIGQLKSDIQLICARAFLDYMTYKRECVYVRLSLLPNNIRESLYGNN
                     RKEEFVQNFSFIGKDDIIFLPEEKSFESENLLIENKKYDLDFYGMIKETWNKLQQEGI
                     KESQIRSVIDEDIQKYSYNLMNTFIYNSSNQTAYNNIVNDSIATTVKYVLQKHDKWSK
                     REGLDKLIKAIALHIQNLIERIKIGNIVKHPNKDEIMKERAYEYNIAKEILSNMSLMY
                     GVEFPEDEAIFLATFLYLSYVGLNEESIAILVIMHGESTASSMVNVANTLLDCNHAVA
                     INMGLEDRVQDILVQAVEIANEIDKGKGILILTDMGSILTFAKVIHDATGKEVKAIDM
                     VSTPIVIEATRKALTPEMTLEKLYFNIIESIKKHYGDMEITYAEKDNGGRYFDRLLID
                     NISKTLTFLNGEKAYFILKEVINRISEHYSLVIQDELLVKFIFHCSCMIERVIIKESL
                     VYKSYEERISRSKELYLVIKESFKLVEETFDIIISDMEYANILDIFESQYDTDKFKS"
     gene            4265..4669
                     /locus_tag="L3M60_001200"
     CDS             4265..4669
                     /locus_tag="L3M60_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225887.1"
                     /translation="MIGIIVMTHGSFSEEIIKSCELIAGPAERTAAIKLNRNDNIEDL
                     NKNFVEKLNELDEGDGVLVLADLLGGSPSNVASLNLKKGGKFHALTGVNLPMLLEALI
                     NREGKSLEELAEACIEAGQTGINNINKVLSSM"
     gene            4693..5190
                     /locus_tag="L3M60_001201"
     CDS             4693..5190
                     /locus_tag="L3M60_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225888.1"
                     /translation="MSLVKYCRCDDRLIHGQVIYKWVKHLGVKKIVVVDDETTNDVIA
                     KGLIKMAAPKNIDLSILTVSESRRYFYNNQADDNVFVLIKNLDTANRMIEEGINIKKL
                     IVGRIPTGIGKKKISQNVYINKKEFLLIDEFIKKNINVSIQMVPDEEEIELNHNINRY
                     KGEFI"
     gene            5193..5594
                     /locus_tag="L3M60_001202"
     CDS             5193..5594
                     /locus_tag="L3M60_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBN6225889.1"
                     /translation="MNKYKVIILCHGTWGKLLVEETNKNFGLSNQYEVLSLSSEKDVS
                     VFMKGVEEAVDKEDVKIIISDLYGGSTSSVAISVAIRRGIDAINGLSLQTILIVDEEL
                     SKEDKLHTLPERIVKKNNNLCVDLIKEFKKI"
     gene            5612..6100
                     /locus_tag="L3M60_001203"
     CDS             5612..6100
                     /locus_tag="L3M60_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225890.1"
                     /translation="MAKITLVRVDHRLIHGQVITKWVKIAQAQKIIIVDDFLGQDEFM
                     ADIYKMAAPSGVEVVILTAEDAGQAFQNNTLGDKNIFILFKNVDMANKAYKAGLKYEK
                     IQLGGIPNEAGKKMVFTAVSLGSEDIKQLNELNENGVEIVLQVIPEESSMTYENALKK
                     FK"
     gene            6127..6882
                     /locus_tag="L3M60_001204"
     CDS             6127..6882
                     /locus_tag="L3M60_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6225891.1"
                     /translation="MESVLMLAIVTGLWYWFAAGLAGYTLFSTLKSPLFIGFSLGLLW
                     GDVTTGMIVGASIEMVYLGMVAAGGNIPSDKCLAALIAIPVALQTGVNAEVAVSIAVP
                     LGVIGVLVNNLRRTGNAVLVHKADKYAEEGNTKGIWRCATLYSLIFGFVLRFPIVFVC
                     NFFGADLVQSLLNVIPQWLMNGLTVMGGILPALGFATTIFTIGKNKFLPMFIIGFFMV
                     QYFEISITAAAIFGVCIALLITFMKEDKRVGEV"
     gene            6882..7745
                     /locus_tag="L3M60_001205"
     CDS             6882..7745
                     /locus_tag="L3M60_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBN6225892.1"
                     /translation="MQEIKNKDVKNGQGTTEVNKTRVLTKKDVTKTYLRWWWTAELSN
                     SFERMQALAVCASFTPALEKLYKKKEDLVDALKRHLQFFNTQAIWGGLIHGTVLAMEE
                     EKATEGKIPGEVISGVKNGLMGPLAGIGDTLDFGTFQTIFLALGASFGAEGSVIGAFF
                     PIIFSILLFCEGYYLFHLGYSLGRDSIKKILSGGIVNKIIDGASILGMFMMGALSATT
                     VKLSTPLSFDIGGKAIVVQDTLNMIAPGLLPLSVVFFVYWGMKYKKWTITKLLVILVV
                     LALVGSFIGIF"
     gene            7792..8271
                     /locus_tag="L3M60_001206"
     CDS             7792..8271
                     /locus_tag="L3M60_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HBN6225893.1"
                     /translation="MSEKMYTEKEYLEGIRGATGERAVWFYLLMKEAEKLGVNPDDIC
                     KEAIYGFGKMRGQKYSVADTPGKMAEMLYNSKGQKVFEMELVENTDENGVLKFHHCPL
                     DAAWKEYGLTKEERKEICRLACYGDYGRVDCAQSVRLEFAQKRAHDDEVCELVFTRK"
     gene            8336..9643
                     /locus_tag="L3M60_001207"
     CDS             8336..9643
                     /locus_tag="L3M60_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBN6225894.1"
                     /translation="MDISRRVDNSLNRTIEFLKELIKIDSQQGEPILQCPFGIGPKKS
                     LEKTLDYCASLGFSVKNIDNYIGYAEIGEGKELIGIPMHLDVVPPGDGWSVDPFSGVV
                     IDNIIYGRGAVDNKGAVSMLIHVLKNIEDMYPTINKRIRLIFGTNEETGMECIKYYLD
                     KGEEIPSMGFTPDAMYPVVNGEKGRVHIRIEKEIKIDKSKPYIILRGGTKENVVPSHC
                     TAKIVNGIILELTTKGVAVHASNPEKGENAISKMVIKILKDNMNFQHREDIELVSKYL
                     CSDYYGNALGINRYDGVFKNTTLNLGILKVNEEKIVCELDIRYGKNIILNNIIDRFKK
                     FFCNGWKIEVIAHKDLHYVDESNLVLKKLLEAYEEVTDENGYTIAMGGGTYASWFKDM
                     VAFGPKFLAYKTGGHGVDERVPINHIRKNMEIYTLALIKLLEL"
     gene            9960..10082
                     /locus_tag="L3M60_001208"
     CDS             9960..10082
                     /locus_tag="L3M60_001208"
                     /inference="COORDINATES: protein motif:HMM:NF021468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HBN6225895.1"
                     /translation="MLSNKIIKKCCKIGYMIREKSIDIYSDSGWEFMVWDENGE"
     gene            10586..11680
                     /locus_tag="L3M60_001209"
     CDS             10586..11680
                     /locus_tag="L3M60_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225896.1"
                     /translation="MNELNIAKTLILKRKEKGITQDELANYIGVSKASVSKWETGQSY
                     LDITFLPQLATYFNITVDELICYEPQMMKEDINKLYNKLCKDFTAKPFDEVMIEIREI
                     IKRYYSCFPLIFRMGLLIVNHYDIVDEKKRELLIDEALEIFIRIQETCNDIDICRQAK
                     NMEATCYILLNQPIQVIDLLQNSNFPMINESILLAQGQMMNGQIDEARETFQLGAYQN
                     LISLVQNLVGILQNADKLQMKEIERRILAISNIFELDTLSPATMLSAYLTQTQINLIH
                     EDNEGAIKSLRKYVDLATSDIYPITIHGDDFFDKLDRWISEIGIGITRDDTIVKAGIV
                     AAIKNNPMFSVLSENKEYKFLIEKLSLLEE"
     gene            11685..11885
                     /locus_tag="L3M60_001210"
     CDS             11685..11885
                     /locus_tag="L3M60_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc_N domain-containing protein"
                     /protein_id="HBN6225897.1"
                     /translation="MENLMEYLPLLIPVIILDLILIITALVHVLRHPNYKIGNKAIWI
                     IVVLFISIIGPILYFTIGRGEE"
     gene            11885..12802
                     /locus_tag="L3M60_001211"
     CDS             11885..12802
                     /locus_tag="L3M60_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225898.1"
                     /translation="MNIVSINGLSKGFGNRKIIDNLNFTVPEGSVFGFVGKNGAGKTT
                     TMKMVLGLLEPDSGTIDVCGEKVTYGGTSSNRHVGYLPDVPEFYNYMRPLEYLSLCGE
                     ITGLSKQEIKIRSEELLSLVGLGNEKRRIGGFSRGMKQRLGIAQALLSRPKLLICDEP
                     TSALDPVGRKEILDIMLKIKDSTTVIFSTHILSDVERICDHVAILNKGSIALSGTLSE
                     IKSMHGKDRLLLEFASNDEIQKFKSSGDIKSLLKYSEETNMEIVLHGKDIKAIQKTVI
                     STLAEMNLCPVKMELIELSLENLFLEVVK"
     gene            12799..13557
                     /locus_tag="L3M60_001212"
     CDS             12799..13557
                     /locus_tag="L3M60_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225899.1"
                     /translation="MREYIAFTKKEFKENLRNYKLFSLIILFLVFGIMSPLSAKFMPD
                     LIAHFAPTLKVTTAPTALDSWTQFSGNISGLGASLTLIVFCNILSNEYSKGTLVIMLT
                     KGLSRSSVILSKFSIAVIIMTIGFWLSFLCTYGYTMYFWPTENLNHIIFSAFNLWLIN
                     IMYITILILGCVLFRPAFASVLLVLVATLILSLISIPKQIAPYTPNFILSKNIDLVSG
                     KVAAPEFIIPMIVTIVISIICLLLAVILFKKKAV"
     gene            14033..15106
                     /locus_tag="L3M60_001213"
     CDS             14033..15106
                     /locus_tag="L3M60_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBN6225900.1"
                     /translation="MEKSKVYFCDLYSNSQNKNVPNNVRRLFDKAGFKDLIEKNDQVA
                     IKLHFGEKGNTTYMSPVAVRQVVDKVKECEGKPFLTDTNTLYTGSRTNSVDHLTTAIE
                     NGFAYAVVNAPVIIADGLYSRNYENVKIDKKHFESVKIGGEIYNSSAMIVMSHFKGHE
                     AAGFGGALKNLAMGCASAAGKQMQHSDVTPVVKEKKCIGCGKCVNSCPTKAISIVDKK
                     AVIDSDVCYGCGECPTVCPTRAVTIQWESDSDVFVEKMAEYAYGAVSNKKDKIGYITF
                     VMNVTPLCDCVPWSGRPIAHDIGILASTDPVAIEQACYDLICKEMGHDVFKHEHPHVN
                     GTRIIDYACEMGMGSKEYELIKL"
     gene            15176..15439
                     /locus_tag="L3M60_001214"
     CDS             15176..15439
                     /locus_tag="L3M60_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225901.1"
                     /translation="MKIYYEKENSKKANVNNIPSVFAFNFLRNFLTSKEYEETRKEYF
                     INNLTKSQVNQAMKDLKWLFREYEGLEVITMESLDGVKTRIVL"
     gene            15645..16622
                     /gene="bioB"
                     /locus_tag="L3M60_001215"
     CDS             15645..16622
                     /gene="bioB"
                     /locus_tag="L3M60_001215"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBN6225902.1"
                     /translation="MEKYIIKLKNKVLGGKEISYEEALNLISLDTNNKNDFDILLKSA
                     NEIREFFMGRKADLCTIINAKSGKCSEDCKFCAQSSHYKTGVEEYSLLDYNEILNRAK
                     EMESKGVHRFSLVTSGKGMSGKEFNDILKIYERLRENTNLKLCASLGIIDYEQAKMLK
                     SVGVTTYHHNVETARDNFHNICTTHTYKDRIKTIKEAKEAGLDVCVGGIIGMNESEEQ
                     RLKMAFEIRELNVKSFPINILNPIKNTPMEDYDVLEPMEILKTTAVFRFIIPNVYIRY
                     AGGRLSLKGYDEVGFNGGVNSAIVGDYLTTVGNGIENDKKMIIEQGFEI"
     gene            16850..17863
                     /locus_tag="L3M60_001216"
     CDS             16850..17863
                     /locus_tag="L3M60_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN6225903.1"
                     /translation="MSITIKEIGELAGVSKTTVSKVINNKDENISQATREKILKIMKE
                     KNYVPNKLAQSLVTKKTNTIGLLIPDIRNPFFTDVSRGVEDKANKEGYNIILCNTDED
                     AKKEYEGIRTLSERMIDGIIFAASSNTNWKEANYKDIKIPTVLIDKKISMNKETLKGI
                     VKINNFEGAYIATKYLLDTGNKKIIYLSGPLQNEIAIDRLEGYKKALIECNLSYNTDY
                     VFEGKYKIEWGQEFIKNLEKIDFDAIFCANDLIAIGVIRGLKERGLSIPNDVSVVGFD
                     DIQTSSLISPSLTTVRQPSYDIGYKASEILINCLKGDKKESFDELIFKPELVIRDSTK
                     ESE"
     gene            17905..18798
                     /gene="rbsK"
                     /locus_tag="L3M60_001217"
     CDS             17905..18798
                     /gene="rbsK"
                     /locus_tag="L3M60_001217"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBN6225904.1"
                     /translation="MGNIVVIGSVNMDMVCSVDKRPEKGETVLGNSFFTSPGGKGANQ
                     AISASKLGANVKMISCVGEDSLGEELIRNFRTNKVDYSLVSRNKHKSSGVAVITLCEN
                     DNSIVVVPGTNELVDIELIKKNEEEIKNADIVLLQLEIPLKTINYVVNFCFENRINVL
                     LNPAPAIKLDEDIIEKVTYLTPNEHEYKIVFDTNEEIEEVLKKYPNKLVITEGKNGAR
                     FYDGNEVKHVSCISVDVQDTTGAGDTFNGALAVAITEGKNLYTAVEYAVVVSGLSVTK
                     LGAQSGMPYKEEVEKYLNNNK"
     gene            18846..19808
                     /locus_tag="L3M60_001218"
     CDS             18846..19808
                     /locus_tag="L3M60_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBN6225905.1"
                     /translation="MKVRKILSLGIAGILAIGMLTGCSMEGPSKSDNKGASDKKDLTI
                     GVSTITLQHQFFIDIDEGIKEKAKELGVKVIVNDPDQDVAKQTSAIEDFIQQNVDGMI
                     VLGTDNSAIVPAVEGAFEKMPVVTVDAVLNTENITSYVGTVSYDAGKKLGEYTKKYID
                     EKLGGKSEIAIVTDLKSQIQMQRIDGFKDALKGSANVKILNSQPGYDREESLNTVENL
                     IQSNPDVDIIYATAENSVLGAKAALESAKNKDVKIVGFDLTEEASSGITDGTILAMIQ
                     QQPKEMGRLAVEAVVKAIKGEKVEKNIPVPALLYDKENIKDFKN"
     gene            19861..21372
                     /locus_tag="L3M60_001219"
     CDS             19861..21372
                     /locus_tag="L3M60_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBN6225906.1"
                     /translation="MSNIILKLSNIAKEFPGVKALDNVNFELFHGEVHALLGENGAGK
                     STMIKILTGAHSKTSGKFIFQDKEIENISPDVSKKIGINAIYQELTVFDELTVAQNIF
                     MGKEINGKVLTNDKKMNEEAKKIFDNMGIDVNPNSLVKELSIAQKQMVEIARVLSSET
                     KVLIMDEPTSSISKKETEILFRLIKDLKDSGVSIIYISHRMEELFEICDRITIMRDGK
                     TVSTLNTKDVSSEEELVNLMIDRKLDQFFPKRKVEIKEEIMRVENLTKNNVFNDVSFN
                     IRKGEILGIGGLVGSKRSEIVEAIFGLRTYDSGKIYLNNEEVKFKTPSDAIENGLGLI
                     TEDRKGTGLFLQMSVKENTTMAGLKKISKFKSIIDRKKEKEILEKYIEALRIKTPHMN
                     QVIQSLSGGNQQKAIIARWLLLQPDILIMDEPTRGIDVNAKAEIYNLMGNLVESGVSI
                     IMISSEIPELISMSDRIMVMREGHISGFLEGEEMVENNVLKLAFGGKINEFNN"
     gene            21356..22354
                     /locus_tag="L3M60_001220"
     CDS             21356..22354
                     /locus_tag="L3M60_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBN6225907.1"
                     /translation="MSSITKHKEEKSSPLLTKLTSAFRDQGSAAVGLVIIFIIMSIAS
                     SNFLTLDNLINVGRQISINAILAVGMTFVIITGGIDLSVGAVIALVGTFWATTVVNYN
                     APIWLGMILALAIGTILGVIKGAIISTQKLPPFIVTLAMLTIISGASFVFTGGRPISV
                     NTDAFKMLGRGYVGPIPIPVIIMIIVVIAGHFLLKRTNFGRHVHAVGGNEEAARLCGV
                     KVNKVIVKVYALAGLLTALAGIILSSRLASGSPTVGDGAELDAIAAVVLGGTNMMGGS
                     GSIVGTCIGVGIIGILGNGLNLLSVSSYNQMIIKGLVMLFAIWINNIKLKKLAKSK"
     gene            22538..23800
                     /locus_tag="L3M60_001221"
     CDS             22538..23800
                     /locus_tag="L3M60_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBN6225908.1"
                     /translation="MKKILLDTLNSKKQEYIDYLKELVSIKTEDVGHGILGGFEKEGQ
                     EYIEKLANYIGFSVDRQEMSEELIKKAKNIYKEGNLGHNYQDRYNLICKYSDDLPGKT
                     IVFNGHVDTMPPGDISKWKYNPYRATEDNGKLYGLGIADMKSGLIASILAVKLIKDSG
                     LNVPGNVKIMSVVDEEGGGNGTINAVMNGIDGDCCIICEPSEHNLIVAHMGFVFFEVE
                     VKGVSLHCGSKWEGINAIEKAMLLLQDIKELEHNWLMIYKHPLLPSPTINLGVINGGT
                     AGSTVPDKCVFNLCVHFLPNIMSYEQVVNDVTNVIMTRANGDLWLKDNKPNINIYQSG
                     LGFEMDKDSDFVVNVHKILEETLGRSLEIKGSTAGNDARLMKNLAEIPTLILGPGSIE
                     QCHSIDEYVEIKEYLDSILMYASLILNL"
     gene            23820..24563
                     /locus_tag="L3M60_001222"
     CDS             23820..24563
                     /locus_tag="L3M60_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HBN6225909.1"
                     /translation="MKKVLIVGESWVKNITHIKGFDTFVTTHYEEAVKWLKEAIESGG
                     YETVHMPAHVAADSFPYKLEELNEYDCIILSDIGSNTFLLSNSTFIDYNSNPDRLELI
                     KEYVNNGGALIMVGGYMSFTGIDAKARFGETAIKDVLPITMIDKDDRVEKPAGIIPEV
                     VDSEHPVLKGIPTEWPKFLGYNKTVARDNCPVLATIGGDPFVAVGEFGKGKSAIFSSD
                     CAPHWGPKEFTDWKYYNKLWVNMLDWLTC"
     gene            24736..26121
                     /locus_tag="L3M60_001223"
     CDS             24736..26121
                     /locus_tag="L3M60_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6225910.1"
                     /translation="MSDLNKKFLIEYIDEIQEELRDISDKIWKNPELQYKEYYASNLQ
                     KEYLKKHDFSIDELEGMDTAFVASYGEGEPVIAVLGEYDALDGLSQKVSITKEPILDS
                     GAGHGCGHNLLGTGSIGAVIAVKELIKLKKLKGTIKYYGCPAEEDLSGKVLMIKKGFF
                     DGIDCAFSWHPFDINTPIRIPTLANYSVKFRYNGISAHAAQAPYNGRSALDAVELMNI
                     GCNYLREHIFDSCRVHYVTTNGGKMPNIVPDFAEVWYYIRGIKMEHVRDVFDRIVDIA
                     KGASLMTGTSMEYDIISGVYDYIPNTILTDILSQNMKLIGVQNYNEDDYHFAEKLAET
                     VSIDKRISVSSVLSGNKDITKMNLHGEVTDDTFTHNNCISLSLDIGDVSYIIPTAQCS
                     CSVWPIGISAHTWQSCASAGSDMGFKAMLLASKSIACSINDVMLDEVIINKAKKELKN
                     TIGSFEYIPII"
     gene            26333..27172
                     /locus_tag="L3M60_001224"
     CDS             26333..27172
                     /locus_tag="L3M60_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225911.1"
                     /translation="MFGYVKINKMDLTFREYDYYKAYYCGLCKYLKRNHGEISRFSLN
                     YDITFLIVLLTAVYNPESISTEEVCIVNPFKKKKVITNDITEYAASMNILLTYYKLED
                     NLMDDRRIKDKLAYYIYKNKLKLAYEKYPEKAEYIKQQLNELNKLEKDKNINIDEVSS
                     IFGNIMGEVFAYKKDENERNLRMIGFNIGKYIYLLDAYEDLDEDFKKGRYNPFIEYID
                     KNDELKEKVKKIIVTSLGFLARGIDNLNLNKNVGIIENIIYSGVYLRYMNILESRGGK
                     NVQ"
     gene            27162..27605
                     /locus_tag="L3M60_001225"
     CDS             27162..27605
                     /locus_tag="L3M60_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225912.1"
                     /translation="MYSNTYNNEHFMRARAFIENNEFKKAYDFLKTLTDKCAEWYYLT
                     GFSAMNIGYYEEGEDFLKRAKFMEPENSEYSDALRSYTQYRNDYSNRADNYNRRRRND
                     LDGCCCCCCDDCCCCLGDDCCENCAKLWCLDSCCECFGGDLITCC"
     gene            27616..28107
                     /locus_tag="L3M60_001226"
     CDS             27616..28107
                     /locus_tag="L3M60_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225913.1"
                     /translation="MSIKVAYGGVLLALNVILLTLTNIIPVNTLFIMGLASLLVSIVI
                     MEWGFKSGIAFYIGSIALGFIVMASKSQWIVYSLTFGIYGIVKYLIEKDRSIYIEYFM
                     KLVFANIMILILYFLLRTIVYIPINIFIIGSFEIAFIVYDYVFSSFIGYYNNRLRKML
                     FKK"
     gene            complement(28309..29037)
                     /locus_tag="L3M60_001227"
     CDS             complement(28309..29037)
                     /locus_tag="L3M60_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HBN6225914.1"
                     /translation="MKNIAAFFDIDGTLYRDSLMVEHFKKLIKYDIIDQKNWYKHARD
                     TFMDWDKRQGNYDDYLLEICDLYVDSLKGLDKTCINFTSDQVIKLKADRVYKYTRSRI
                     EWHLNQGHIVIFISGSPGFLVEKMAKKYNVTDFLGSDYVFENNIFTGTVIPMWDSISK
                     NNAINDFVVKYDLDLSNSYAYGDTNGDINMLKRVGNPIAINPTKELLSQISLDEEISK
                     NVEIIVERKDIIYSLSTDVNIVDI"
     gene            29162..30250
                     /locus_tag="L3M60_001228"
     CDS             29162..30250
                     /locus_tag="L3M60_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225915.1"
                     /translation="MNFNFAKATNSRLMGSLGLMINWIDDENNHFCQYFLLDAEGLGL
                     ADYVSLKNPTQEEAYMEEERLMGGFGSDRVELTKDESLFLVSYFGNKNLYYNKLLPGD
                     KCEYIDIIKNYKTDLTIEKLYDKICKRVDEEVEFINYMTMRFIAWDRESLKYFSGSDE
                     IANMHITNINGTLLKNIVSDKGQGRYISEALYEDNDGYYICKIAFCISKCYETGFRIN
                     SLLVTDKEPMYDFEVFDEISKSEFVSVYSVNSPEEFAGVFYRENPFLLKSDMNEGIFF
                     TRFNFNNDHVKENVYVINNDMKAIYYLMGNKFFIGTYDENDCNYINEISQSNYSDYIK
                     FEENFFFEQNALYDFAESGSLDFDDFLE"
BASE COUNT        11910 a         3071 c         5585 g         9743 t
ORIGIN      
        1 atataaatta atagataaga tataatttta aaaggagtga tataagatga gcaaatatag
       61 ttctttatgg gaatatgtta agaaaaacaa tagtcaatcg tttaaattaa cttttgaaga
      121 aataaaagat attgctggag tagagataga ccattcgttt ttgaaatata aaaaggaatt
      181 aaatgaatat ggatatcaag taggaaaaat ctctttaaaa gagaagactg ttattttcga
      241 taaaattggt taagacataa ataattacat ataataaaat aatttgtatt gacaactatt
      301 aaaaataaag taaatcttat ataagattta ctttattttt agaggctgct ttttttaact
      361 ttatactata aggtatataa aactaagcct attaaaaatg ttataatttc tattgctatc
      421 atagaccata tcaatccatc aagttcataa aagtaattaa tctttattaa atagcttctt
      481 tattataatt tctttatttc atataatata aatagtatta aaatttttat gagagttaga
      541 gtaagcatgt attactactt aaatatttct ataatcaaca tctagtaatt tacctttttg
      601 gtgaaaatga ttaataagtt ttttgggatg attcattatt aattcatctg gaaaattaat
      661 ttcctttaag atattctcag aatactcata gttacctatg tcaaaacaaa tgtgagcatc
      721 tgaagttagt atgattttag caccatattt tttgcaaagc gtagcaatat acttacagtt
      781 atccgagcta ccatttcttg acactccttt aatagaacaa ttatttattt ctattaagat
      841 attcttttct atagctaatt taatgattaa ttcatagtcg attgggtagt ttgggtttcc
      901 aaggtgtcct aatatttcaa tattatcatg cttatttatt gcatttatta aggctttagt
      961 attattctct aatgtatttg gtgaaaaaac ggcttcgtga aatgacaaga taagataatc
     1021 taatcgagga attacaaaag gctctaggtc tatgtttcct ttagtgtcta gtatatttgc
     1081 ttcacatcct cttaaaatta taatgttatt tatgattctt gggatatttt taatgttgtg
     1141 aaagtaccat ctgtggggag aaccaggcat cttaggacca tgttcagatg tacctagtat
     1201 cttaattcca ttttctttac aataattaat attttctaac aaagtgctat agccatgacc
     1261 actgactata gagtgggtgt gtaaatctga taaaaaattc atataaattc ctcctctata
     1321 aaagatgagg gtaacgtaat ttatattata aaacgaatag aaaagaaaat caatagttac
     1381 ataattagac aaaaatatta aaatattgta gaattaagct taataataaa tgtttaacag
     1441 tattactgct tataggggag ggtaactatg acacaaagaa agctatcaag gaaagaaaaa
     1501 gttttaattt gcttgaaaag gttaacagaa gaacacttga actttaataa tggcattaga
     1561 tttggatttg atgcagaata tattgctact gaagtaggta taaatagaag taatgctagt
     1621 aaggaattaa atcaattaat aaaagaaagt attattataa agataaaggg aaagcctgtt
     1681 caatacttat acaaagaaag tattgagagc attttaggaa agaaactaaa taaagggata
     1741 tttagtaata ttaactctat aaatgaatat agtgaagatg attctatcaa agatgagtat
     1801 atcttagaag gtataaaaaa agagaacaat atttttaatt tagtaggatg tgatggaagt
     1861 ttaaagtccc aaatagaaca ggctaaagca gcagtaatat atccacctaa ggggttgagt
     1921 acattaataa taggacctac aggagttggt aaatctatat ttgcagaata tatgtataag
     1981 tatagtttga atataaaaga agatagtgat aatgcaccat ttatcatatt taactgtgct
     2041 gattattctg ataatcagca attattgtta gcacagcttt ttggatatgt aaaaggagca
     2101 tttactggag ctgataaaga caagtatggt attgtgcatg aagctaataa tgggatattg
     2161 tttttagatg aagttcatag gttatctgca gagggacaag aaatgctatt tctgcttatg
     2221 gataaaggta tatatagaag gttaggagag gctaataagg tacatgagtg tagggttatg
     2281 ataattgcag ctactacaga agacccagaa acagcaatgc tggaaacgtt tttaagaaga
     2341 atacctgtaa ctataaagat tccgtcttta gaggaaagag gttttcaaga gagaatgaaa
     2401 cttatatgtt atttttttaa agaagagagt attaggattg gtgttaagtt aaaggtttca
     2461 aaagaagtta ttaaggcatt tattttatat aagtgtaagg gaaacatagg acaattaaaa
     2521 agcgatatac aattaatttg tgcaagagct tttttagatt atatgaccta taaaagggaa
     2581 tgtgtatatg taaggctctc tcttctacca aacaatatta gagaatcatt atatggaaat
     2641 aatagaaaag aggagtttgt acaaaatttc agttttattg ggaaagatga tattattttt
     2701 ttaccagaag aaaagagttt tgaaagtgaa aatttattaa tagaaaataa aaaatatgat
     2761 ttagatttct atggaatgat aaaagaaact tggaataaac ttcaacaaga aggtattaaa
     2821 gagtcacaaa ttagaagtgt tatagatgaa gatatacaga agtattccta caatttaatg
     2881 aatactttta tctataatag tagtaatcaa acagcttata acaacattgt taatgattca
     2941 atcgcaacta ctgtgaagta tgttttacaa aagcatgata aatggagcaa aagagaagga
     3001 ttagataaat taataaaagc tatagcctta catattcaga atttaattga aagaataaag
     3061 attgggaaca tagtaaagca tcctaataaa gatgaaatta tgaaggaaag agcatatgaa
     3121 tataacatag caaaagaaat attatctaac atgtcattaa tgtatggagt tgagtttcca
     3181 gaagatgaag caattttttt agcaacgttt ctttatttat cttatgtggg attaaatgag
     3241 gaaagtatag caatattagt cataatgcat ggtgaatcta cagcctctag catggtgaat
     3301 gtagctaata ctttactgga ttgtaaccat gcagtggcaa tcaatatggg attagaagat
     3361 agagttcagg atattttagt acaagctgta gaaatagcca atgaaattga caaaggaaag
     3421 ggaattttaa tcttaaccga tatggggtct attttaacat ttgctaaagt tattcatgat
     3481 gctacaggaa aagaagtaaa agcaatagat atggtaagta cacctattgt aatagaagca
     3541 actagaaagg ctctaactcc agaaatgaca ttagagaaat tatattttaa tattatagaa
     3601 tctataaaga aacattatgg agatatggaa attacatatg ctgaaaaaga taatggtggt
     3661 agatattttg atagattact aattgataat attagtaaaa ctttgacttt tttaaacggg
     3721 gaaaaggctt attttatatt aaaagaagta attaatagaa ttagtgaaca ttattcttta
     3781 gtaattcaag atgagctttt agtaaagttt atatttcatt gttcctgtat gattgaaaga
     3841 gtaataataa aagagtcttt agtttataaa tcatatgaag aaagaattag tagaagtaaa
     3901 gaattatatt tagttataaa agaaagtttt aagctagtag aagagacatt cgatataatt
     3961 atttcagata tggaatatgc aaatatatta gatatatttg aatcacaata tgatacagat
     4021 aaattcaaaa gttgatacaa aatttaaaaa tagccaaaag gctatctttt ttttactttt
     4081 tttgatacag atttgataca agttggaaaa atcttttgat acatattgat atagtaagtt
     4141 ttttaaaaaa tttaagcaaa aaggtttgat taaatcctgt aaaatagcca tttgttattg
     4201 atacacataa gttggcacgg caattgcttg tataaaaaat gacaagaaaa gtgaggtgaa
     4261 attggtgata ggaataatag ttatgactca tgggagtttt agtgaagaaa ttataaaaag
     4321 ctgtgaactt attgcaggac cagcagaaag aacagcagca attaaactaa ataggaatga
     4381 taatattgaa gacttaaata aaaattttgt agaaaagtta aatgaactag atgaaggtga
     4441 cggagtttta gtattagctg acctattagg aggttcgcca agtaatgttg caagtttaaa
     4501 ccttaaaaaa ggtggaaagt ttcatgcact tactggagta aatcttccaa tgctacttga
     4561 agctttaatt aatagagagg gaaaaagttt agaggagtta gcagaagcat gtattgaagc
     4621 aggacaaact ggtataaaca atattaataa agttcttagc tcaatgtaat agaaaaagga
     4681 gtttgattaa aaatgtcttt agtaaaatat tgcagatgtg atgatagatt aatacatggt
     4741 caagtgatat ataaatgggt taagcatcta ggtgtaaaaa agatagtagt agtagatgat
     4801 gaaaccacca atgatgttat tgctaaaggt ttgataaaaa tggcagcacc aaagaatatt
     4861 gatttatcaa tattaactgt aagtgaatca aggagatatt tttataataa tcaagctgat
     4921 gataacgttt ttgtattaat taaaaattta gatactgcaa atagaatgat agaagaaggg
     4981 attaatatca aaaaacttat tgttggaaga atacctacag gaataggaaa aaagaaaata
     5041 tctcaaaatg tatacattaa taaaaaagag tttcttttaa tagatgaatt tattaaaaaa
     5101 aatattaatg tatcaattca aatggtacct gacgaagaag aaatagaatt aaaccacaat
     5161 attaatcgct ataaaggaga gtttatttag caatgaataa atataaggta atcatactct
     5221 gtcatggaac ttggggaaag ttattagtag aagaaacaaa taaaaacttt ggattgtcaa
     5281 atcaatatga agttttatca ttaagttctg aaaaagatgt ttctgtattc atgaaagggg
     5341 ttgaagaagc agtagataaa gaagatgtga aaattataat atcagattta tatggagggt
     5401 ctacatcaag tgttgcaata tctgttgcta taagaagagg aatagatgca ataaatggac
     5461 ttagcttaca gacaatttta atcgtagatg aggaattaag taaagaagat aaattacata
     5521 cattaccaga aagaatagtc aaaaaaaata ataatctttg tgtggattta attaaagagt
     5581 ttaaaaaaat ataaattatg aggtgaatat aatggcaaaa ataacattag taagagtaga
     5641 ccacagatta attcatggac aagttataac aaaatgggta aaaatcgctc aagctcaaaa
     5701 aataataata gttgatgact tcttaggtca agatgaattt atggctgaca tatataaaat
     5761 ggcggcacca tcaggagtgg aagtagttat tttaacagca gaagatgcag gacaagcatt
     5821 ccaaaacaac actttaggag ataaaaatat atttattcta tttaaaaatg ttgatatggc
     5881 aaataaggct tataaagctg gacttaaata tgaaaagatt caattaggag gtattccaaa
     5941 tgaagcagga aagaagatgg tatttacagc tgtttcatta ggaagtgaag atataaaaca
     6001 acttaatgaa cttaatgaaa atggagttga gatagtatta caagttatac cagaggaatc
     6061 atctatgaca tatgaaaatg cattaaagaa atttaaataa aaaatataaa ttaggaggaa
     6121 ttataaatgg aatctgtttt gatgttggca attgtaacag gattatggta ttggtttgca
     6181 gcaggtcttg cagggtatac ccttttctca acactaaagt ctcctttatt tataggtttt
     6241 agccttggat tactttgggg tgatgtaaca actggtatga ttgttggtgc aagtatagaa
     6301 atggtttatc taggaatggt cgcagcagga ggaaatattc catcagataa atgtctagct
     6361 gctcttattg caataccagt agcacttcaa actggtgtta atgcagaagt tgcagtatct
     6421 attgcggtac ctttaggcgt tataggagta ttagttaata acttaagaag aactggtaat
     6481 gctgtattag ttcataaggc agataagtat gctgaagaag gtaatacaaa aggtatctgg
     6541 agatgtgcaa cactttattc tttgatattt gggtttgttt taagatttcc aatagttttt
     6601 gtatgtaact tttttggagc agatttagtt caatcattat taaatgttat accacaatgg
     6661 ttaatgaatg gattaacagt aatgggaggt atattacctg cattaggttt tgcaacaaca
     6721 atatttacta taggtaaaaa taaattctta ccaatgttta ttataggatt ttttatggtt
     6781 caatattttg aaatatcaat tactgcagct gcaatatttg gtgtttgtat agctctttta
     6841 attaccttta tgaaagaaga caaaagggta ggtgaagtat aatgcaagaa ataaaaaata
     6901 aagacgttaa aaatggacaa ggtacaactg aagttaataa gacaagagta cttacaaaga
     6961 aagatgtaac aaaaacatac ctaagatggt ggtggacagc agaattatct aactcttttg
     7021 aaagaatgca agcattagca gtttgtgcaa gtttcactcc agctttagaa aagttatata
     7081 agaaaaaaga agatttagta gatgcgttaa agagacactt acaatttttt aatactcaag
     7141 ctatatgggg agggttaatt cacggtacag ttttagctat ggaagaagag aaagctactg
     7201 aaggaaaaat cccaggagag gtcatttcag gtgttaagaa tggtcttatg ggacctttgg
     7261 ctggaattgg agatacttta gattttggta catttcaaac aatattctta gcgttaggtg
     7321 cttcatttgg agcagaagga agtgttatag gtgcattttt cccaataatc tttagtatat
     7381 tactattttg cgaaggttat tacttattcc atttaggata ttctttaggt agagattcaa
     7441 ttaagaaaat tctatcaggt ggtattgtca ataagataat tgatggcgct tcaattttag
     7501 gaatgtttat gatgggagca ttatctgcaa ctacagttaa gctttcaact ccgctatcat
     7561 ttgatattgg tggtaaagca attgttgttc aagatacatt aaatatgatt gctccaggtt
     7621 tattaccact tagtgttgta ttcttcgtat attggggaat gaaatacaag aagtggacaa
     7681 taactaagtt attagtaatt ttagttgtat tggcattagt aggatcattc attggaatat
     7741 tctaatttac aatgattaaa atatagaatt taattttagg aggagaaaga aatgtcagaa
     7801 aaaatgtata cagaaaagga atatttagaa ggaattagag gagcaacagg agaaagagca
     7861 gtatggttct atcttcttat gaaagaagct gaaaaattag gagttaatcc agatgatata
     7921 tgtaaagaag caatatatgg atttggtaaa atgagaggtc aaaaatatag cgtagcagac
     7981 actccaggaa aaatggcaga aatgttatat aacagtaaag gacaaaaagt atttgaaatg
     8041 gaattagtag aaaatacaga tgagaatgga gttttaaaat tccatcactg tccattagat
     8101 gcagcctgga aagaatatgg attaactaaa gaagaaagaa aagaaatttg tagattagct
     8161 tgttacggtg attatggaag agttgactgt gcacaaagtg ttagattaga gtttgctcaa
     8221 aaacgtgctc atgatgatga agtttgtgaa ttagtattca caagaaaata atttttaatg
     8281 ctaaaagcct gttctttaag gacaggcttt aaaaagatag aaagtaggtt actttatgga
     8341 tataagcaga agagtagata atagtttaaa tagaaccata gaatttctaa aagaattaat
     8401 aaaaattgac tcacaacaag gtgaacctat tttacaatgt ccttttggta taggacctaa
     8461 aaaatcttta gagaaaactt tagattactg tgctagttta ggttttagtg ttaagaatat
     8521 tgataattat attggatatg cagaaattgg agaaggaaag gagttaatag ggattccaat
     8581 gcatttagat gtagttccac caggagacgg gtggagcgta gacccatttt cgggagtagt
     8641 aattgacaat attatttatg gaagaggtgc cgtagataat aagggggctg tatctatgct
     8701 tatacatgta ttgaaaaaca tagaggatat gtaccctaca attaataaaa gaataagatt
     8761 aatttttggt acaaatgagg aaacaggcat ggaatgtatc aaatattact tagataaagg
     8821 tgaagaaatc ccaagtatgg gatttactcc agatgctatg tatccagtag ttaatggaga
     8881 aaaaggaaga gttcatataa ggatagaaaa agagattaaa attgataaaa gtaaacctta
     8941 cataatatta aggggaggaa ctaaagaaaa tgttgttcca tctcattgta ctgcaaaaat
     9001 tgttaatgga attattttag agctaaccac taaaggagta gctgtacatg caagtaatcc
     9061 tgaaaaggga gaaaatgcta tatctaaaat ggtaataaag atattgaaag ataatatgaa
     9121 ttttcagcat agagaagata tagaattagt ttcaaaatac ctttgtagtg actattatgg
     9181 aaatgcatta gggattaatc gatatgatgg ggtattcaaa aacactacct taaatttagg
     9241 aatattaaaa gtaaatgagg aaaaaattgt atgtgagttg gatataaggt atggtaaaaa
     9301 tataatttta aacaatataa tagatagatt caaaaagttt ttttgtaatg gttggaaaat
     9361 agaggttatt gcccataaag acttacatta tgtggatgag agtaatttag tacttaaaaa
     9421 attattagaa gcatatgaag aagtaacaga tgaaaatgga tatacaattg ctatgggtgg
     9481 tggcacatat gcaagttggt ttaaagatat ggtggcgttt ggacctaagt ttttagcgta
     9541 taaaactggt ggtcatggag tagacgaaag agtaccaata aatcatatac gaaagaatat
     9601 ggagatatat accttggctt taataaaact attagaattg taaaaagaga agagatttta
     9661 tgtgaaaatg tacggatatc ttaaaaagag agataaaacg agtttaactt tatttaaatg
     9721 ttcaacacca tcaaatgggg ctgtctcaaa atatagataa gtctcttcct tattgttgaa
     9781 atgatgaatt ttacttcata caaaaaagag tgtcttatga agtaaaatgt tcattttgaa
     9841 acacactcat ttgatgatgt tgaagagaaa ataagtatga aattattaaa aaagatgatt
     9901 ttataaaagt taaggttgaa aagttaattg aatggaatta gctaaatgga gaaggctata
     9961 tactatctaa taaaataata aaaaagtgtt gtaaaattgg ttatatgatt agagaaaagt
    10021 ctattgatat ttattcagac agtggatggg aatttatggt ttgggatgaa aatggagaat
    10081 aataaaaagt atagtttata caatttaata agatatatgt acaagcagtt aatataaatg
    10141 attgactgct tttcttttat agatttttat ataatagaca attactaata catgatagtt
    10201 gcctattata attataagaa taaatacgaa taataatttt ataaaagaat taaatggaaa
    10261 attgaagagt tatattcagg attttaaaaa gaatatgaaa tgagaaatga aaataaggtg
    10321 ctttatatta aaagatattt tatacaaaat atttaaaaac gtgataaata aaactgtatt
    10381 tagatagatt aattactagt tttatataga atatggattt tgtttacaaa attagaatat
    10441 tttacaagtt atttttaaaa aatatttaaa tatatatttt agatatttgt ttctctgtgg
    10501 ttgatttttt cgtcaaaagt caacttcaaa tcgcttgtta gctttatgtg tttaaggata
    10561 taattataca taaggaggga ttgacatgaa tgaattaaac atcgcaaaaa cgctgatttt
    10621 aaagcgtaaa gaaaaaggaa ttacacaaga tgaacttgca aattatatag gcgtttctaa
    10681 agcttctgta tctaaatggg aaactggaca aagttatctt gacataacct ttttaccgca
    10741 actcgcgaca tatttcaaca ttacagttga tgagttaata tgctatgaac ctcaaatgat
    10801 gaaggaagat attaataaac tatacaataa actgtgcaaa gactttacgg ctaaaccatt
    10861 tgatgaagtc atgattgaaa taagagaaat cataaaaaga tattattcat gttttccttt
    10921 gatttttcgg atgggtcttt taatagtcaa tcactatgac attgtagatg aaaaaaagcg
    10981 tgaactatta attgatgaag cattggaaat atttattcgt atacaggaaa cttgtaatga
    11041 tatagatatt tgccgtcaag caaaaaatat ggaggcaact tgttatatat tgttaaatca
    11101 acccatacaa gtgattgatt tattgcaaaa tagcaatttt cccatgataa atgaatctat
    11161 acttcttgca caggggcaaa tgatgaatgg tcaaattgat gaagcaagag aaacatttca
    11221 acttggggca tatcagaatc ttatatcact agttcaaaat ctagtgggta ttttgcaaaa
    11281 tgcagataaa ttacaaatga aagaaataga aagacgtatt ctagcaattt caaatatatt
    11341 tgaacttgat acattatctc ctgctactat gctttctgca tatttaacac agacacaaat
    11401 taatttaata catgaggata atgaaggagc tattaaatca ttacgaaaat atgtagattt
    11461 agcaacaagt gatatatacc caattacaat acatggtgat gacttttttg ataaacttga
    11521 cagatggatt tctgaaattg gaataggtat tacacgtgat gatacaatcg taaaagcggg
    11581 tattgttgct gctattaaaa ataatccaat gttttctgta ttaagtgaaa acaaagaata
    11641 caaatttctt atagaaaaat tatctttatt ggaggaataa gattatggaa aatttgatgg
    11701 aatacttacc acttttgatt cctgttatta ttttagattt aattcttata attactgcac
    11761 ttgttcatgt attgagacat ccaaattata aaattggaaa taaagcaata tggattattg
    11821 tagtgctgtt tattagcata ataggaccaa ttttgtattt tacaatagga agaggtgaag
    11881 aatgatgaat attgtctcaa taaatgggtt atcaaaaggt tttggtaatc gaaagataat
    11941 agataatcta aattttactg taccagaagg gtcagtcttt gggtttgtag gtaagaatgg
    12001 tgcaggtaag actacaacaa tgaaaatggt acttgggtta cttgaaccag atagtggaac
    12061 tatagatgtt tgtggtgaga aagtgaccta tggaggaaca agttctaatc gtcatgtagg
    12121 ttatctgcct gatgttccag agttttacaa ctatatgaga ccacttgaat atctttctct
    12181 ttgtggcgaa attacgggac tttcaaaaca agaaataaag ataaggagtg aagagttgtt
    12241 atcacttgtg ggacttggca atgaaaagag acgaataggt ggattttctc gtggaatgaa
    12301 gcaacgactt ggtatagctc aagctttgct ttcacgtccc aaattactta tttgtgatga
    12361 accaacaagt gcacttgacc ctgttggtcg aaaagaaata ctagatatta tgcttaaaat
    12421 caaagactca acaactgtaa tattttcaac acatatacta tcagatgttg aaagaatctg
    12481 tgaccatgtt gctatattaa acaaaggaag cattgctctt agtggtacac tttcagaaat
    12541 aaaaagtatg cacggaaagg atagactttt actggagttt gcaagtaatg atgaaattca
    12601 aaagtttaag tctagtggtg acattaaatc cttactaaaa tattcagaag aaactaatat
    12661 ggaaattgtt ttgcatggta aagatataaa agcaatacaa aaaacagtta tatctacact
    12721 cgcagaaatg aatctttgtc cagtaaagat ggaactaata gaattgtcac ttgaaaattt
    12781 atttttggag gtagtaaagt gagagaatac atagcattta caaaaaaaga atttaaggag
    12841 aacttaagaa attataaatt atttagtttg attattttat ttttagtatt tggaataatg
    12901 agtcctcttt ctgcaaaatt tatgccagac ttgattgctc attttgctcc aactttaaaa
    12961 gttactactg caccaactgc tttagattct tggacacagt tttctggcaa tatttctgga
    13021 cttggagcga gtttaactct aattgttttt tgtaatattt tatcaaacga atactctaaa
    13081 ggaacactcg ttattatgtt gactaaaggg ttatcacgtt catctgtgat attgtctaaa
    13141 ttttctattg cagtaatcat tatgacaatt ggtttttggt tgagtttttt atgtacttat
    13201 ggatatacaa tgtatttttg gccaactgaa aatcttaatc atattatatt ttcagcattt
    13261 aacttatggt taattaatat tatgtatatt accatattaa ttcttgggtg tgtattattt
    13321 agaccagcat ttgcaagtgt gttacttgta ttagtagcaa ctcttatttt gagtttgatt
    13381 tctattccta aacagattgc accttatacg cctaatttca tattatcaaa aaatatagat
    13441 ttggtttcag gtaaagtggc agctccagaa tttattattc caatgattgt aacaattgtt
    13501 atttcgataa tatgtttgtt attagctgtc attttattta agaaaaaagc agtttaagga
    13561 tatttttatt attaaatata aatagtactg tattatggaa aaatgtgttt agtgttgtag
    13621 aagaatatat ttaagtaaaa agaggttgtc tcaaaataga gatataagcc tcttttttat
    13681 tgttaaaatt ataaaaacat atttgatttc atacaaaaca agtatctcat aagctaaaaa
    13741 attcattttg agacatttct ttttttattt tagtcattct cattgtactt tataataatt
    13801 aagttattac cttagggaat tgtttaaaaa taaaaaagag aaggattata atctaatgat
    13861 tataagttat ttaagctatg tttagtatat agagtctaaa gtagtaaagg attaaaataa
    13921 tattgtttat atcaaataaa gataagaatc tttaattgtg gtaaaatata ctaaaaaatc
    13981 actttaaaag tgataaaatt attttaagta aatattttaa aggggtgtag atatggaaaa
    14041 gtcaaaagta tatttttgtg atttatattc aaattcgcaa aataaaaatg taccaaataa
    14101 tgtaagaaga ttatttgata aagcaggatt caaggattta atagaaaaga atgaccaagt
    14161 tgcaattaaa cttcactttg gagaaaaagg aaatactaca tatatgagtc ctgtggcagt
    14221 tagacaagtg gttgataagg ttaaagagtg tgaaggaaag ccatttttaa cagatacaaa
    14281 tacactatat acagggagta gaacaaattc tgtagaccat cttacaactg ctatagaaaa
    14341 tggatttgct tatgcagttg taaatgcacc agtaataatt gcagatggat tatatagtag
    14401 aaattatgag aatgtaaaga tagataaaaa acattttgaa agtgtaaaaa taggtggaga
    14461 aatttataat tcctctgcaa tgatagtaat gagtcatttt aaaggtcatg aagcagcagg
    14521 atttggagga gcattaaaaa acctagctat gggatgtgca agtgcagcag gaaaacaaat
    14581 gcaacattct gatgttaccc cagtagtaaa ggaaaagaag tgtataggtt gtggaaagtg
    14641 tgttaattct tgtcctacaa aagctataag tatagtggat aaaaaagcag ttatagattc
    14701 tgatgtatgt tatggatgtg gagaatgtcc aactgtctgt ccaactagag cagtaactat
    14761 acaatgggaa agtgactcag atgtgtttgt cgaaaagatg gcagaatatg cttatggagc
    14821 agtatcaaat aaaaaagaca agataggata tataactttt gttatgaatg taactccact
    14881 ttgtgattgt gtaccttggt caggtagacc aattgcccat gatataggaa tattagcatc
    14941 aacagatcct gtagctattg aacaagcatg ttacgatttg atttgcaagg aaatgggtca
    15001 tgatgttttc aaacatgaac atcctcatgt aaatggaact agaataatag attatgcatg
    15061 tgaaatgggt atgggaagta aagaatatga attaataaaa ttataagaat agtagtactt
    15121 aaatgcatat aattattgta agagaaatct aaaagtatca aaggagtatg catttatgaa
    15181 aatttactat gagaaagaaa acagcaagaa ggccaatgta aacaatattc caagtgtatt
    15241 tgcgtttaat tttctaagaa actttttgac tagcaaagag tatgaggaaa ctagaaaaga
    15301 atattttata aataatctaa caaaaagtca ggttaatcaa gctatgaagg atttaaaatg
    15361 gctatttaga gaatatgaag gcttagaggt cattacaatg gaaagcttgg atggagttaa
    15421 gacaaggata gttttataaa ctagcaagaa ttatatttta ataaaaaata ctgtttcaat
    15481 aataatatgt ataatccatg tactaatatt attttgaagc agtatttttt tgcaaatagt
    15541 aaacttatac ataaaataaa gttgaccaat aaatattaag tatgtaatac tatatataat
    15601 aggtaaacta aatataggta ttaagttgac aaataggagg gtatatggaa aaatacataa
    15661 taaaattaaa aaataaagta ttgggaggaa aagaaataag ttatgaagaa gctcttaatt
    15721 taataagtct tgatacaaat aataagaacg attttgatat tctattaaaa tctgcaaatg
    15781 aaataagaga attttttatg ggaagaaaag cagatttatg tacgataatt aatgctaaat
    15841 ctggcaaatg tagtgaagac tgtaaatttt gtgcccagtc atctcattat aaaactggtg
    15901 tagaagaata ttcattgtta gattacaatg aaatcttaaa tagagctaaa gagatggaga
    15961 gtaaaggagt acatagattt tcattggtta caagtggcaa aggtatgtca ggaaaggagt
    16021 ttaatgatat tttaaaaatc tatgaaagat tgagggaaaa tactaattta aaattatgtg
    16081 catctttagg aattatagat tatgagcaag ctaaaatgtt gaaatcagta ggtgtaacta
    16141 cgtatcatca caatgttgaa acggctagag ataattttca taatatatgt acaacacaca
    16201 cttataaaga taggataaaa acgataaaag aggctaaaga agctgggcta gatgtatgtg
    16261 taggtggtat aattggaatg aatgagagtg aagaacagag actaaaaatg gcttttgaaa
    16321 tacgagaatt aaatgttaaa tcatttccaa taaatatttt aaatcctatt aagaatacac
    16381 caatggaaga ttatgatgta ttagaaccaa tggaaatatt aaaaacaaca gcagtattta
    16441 ggtttataat tcctaatgta tatataagat atgctggtgg aaggctatca ttaaagggat
    16501 atgatgaagt tggatttaat ggaggagtaa attctgccat tgttggtgat tatttgacaa
    16561 ctgttggaaa tggcattgaa aatgataaaa aaatgattat agagcaagga tttgaaattt
    16621 aataaatatt acttaaatta attaatatac ttttagataa ttataaaagt atattaattt
    16681 tttttgtgga ttaatttaaa tattaaattt gaattataaa ttgcaatatt ttgaaaaaag
    16741 ggaaaaatat caaaatttat ataaaatagt tgcaaaggaa aaaaagatgt gatagtatct
    16801 aggtaaccgg ttaagtaaaa cggtttccta aattgtgggg gtgaacttct tgagtataac
    16861 aataaaagaa attggcgaat tggctggtgt ttctaaaaca actgtgtcaa aagtcattaa
    16921 taataaagat gaaaacataa gccaggcaac aagagaaaaa attttgaaaa taatgaaaga
    16981 aaaaaattat gtgccaaata aattagcaca aagtttagta actaaaaaaa ctaatactat
    17041 agggcttctt ataccagata tacgaaatcc attctttaca gatgtatcaa gaggtgtaga
    17101 agataaagcc aataaagaag gctacaatat aattttatgc aatacagatg aagatgctaa
    17161 aaaagaatat gaaggaataa gaacgctatc agagaggatg atagatggaa ttatatttgc
    17221 agcctcttca aatacaaact ggaaagaagc aaattataaa gatataaaaa taccaactgt
    17281 gttgattgat aagaagataa gcatgaacaa agaaacctta aaaggaattg ttaaaataaa
    17341 taactttgaa ggggcctata tagctacaaa atatttatta gatactggaa ataagaaaat
    17401 aatataccta agtggtccac ttcaaaatga aatagctata gatagattag aaggatataa
    17461 aaaggctcta atcgagtgta atttaagcta caatactgat tatgtatttg aaggaaaata
    17521 taagattgaa tggggacaag aatttataaa aaatttagaa aagatagatt ttgatgcaat
    17581 attttgtgca aatgatttga ttgcaatagg tgtcataaga ggtcttaaag aaagaggatt
    17641 aagtatacca aatgatgtga gtgttgtggg atttgatgat attcaaacat caagtctaat
    17701 aagtccttct cttaccacag taagacaacc atcttatgat ataggatata aagccagtga
    17761 aatattgata aattgtttaa agggagataa aaaagaatcg tttgatgaat tgattttcaa
    17821 accagaacta gtcataagag attcgacaaa agaaagtgaa taacagcatg aaagacataa
    17881 atagatatgt aaaggagtaa tgtaatggga aacatagttg taataggaag tgtaaacatg
    17941 gacatggtat gttctgtaga taaaagacca gaaaaaggag aaacagtatt aggtaatagt
    18001 ttttttacat cacctggggg aaaaggtgct aatcaagcta tctcagcttc taaactagga
    18061 gcaaatgtaa aaatgatatc atgtgtaggg gaagatagcc taggagagga gttaataaga
    18121 aattttagaa ccaataaagt tgattatagc ttagtatcca gaaataagca taaaagctct
    18181 ggtgttgctg ttataacatt atgtgaaaat gataatagta ttgttgttgt accagggacg
    18241 aatgagttag tagatataga attaattaag aagaatgaag aagagataaa gaatgcagat
    18301 atagtattgt tacaattaga gattccatta aaaacaataa attatgtagt aaatttctgt
    18361 tttgaaaata gaattaatgt tttattaaat ccagcaccag caataaaatt agatgaagat
    18421 ataatagaga aagtaactta tttaacaccg aatgaacatg aatataagat agtttttgac
    18481 acaaatgaag agatagaaga agtattaaaa aaatatccaa ataaacttgt aataacagaa
    18541 ggaaaaaatg gagctagatt ttatgatgga aatgaagtta aacatgtatc ttgtataagt
    18601 gttgatgtcc aagataccac aggagcagga gatacattta atggagcttt ggcagtagcc
    18661 ataacagaag gtaaaaatct atatacagca gtagaatatg cagtagtagt atcaggtctt
    18721 tctgtaacga aattaggtgc acaatctggt atgccttata aagaagaggt tgaaaaatat
    18781 ttaaataaca ataaataaaa atctaatttg ttcaaattta aaactattaa aagtggaggt
    18841 taattatgaa agtgagaaaa attttatctt taggaattgc tggaatacta gcaataggaa
    18901 tgttaacagg gtgttcaatg gaaggaccaa gtaaatcaga caataaaggt gcttcagaca
    18961 agaaagattt aactattgga gtatctacaa taactcttca acatcaattt tttatagata
    19021 ttgatgaggg aataaaagaa aaagctaaag aattaggtgt aaaagttata gtaaatgacc
    19081 cagaccaaga tgtagctaag caaacatcag caatagaaga ttttatacaa caaaatgtag
    19141 atggaatgat agtacttgga acagataact cggctatagt accggcagta gaaggagcat
    19201 ttgaaaaaat gcctgtagtt acagttgatg ctgtattaaa tactgaaaat attacaagtt
    19261 atgtaggaac agttagttat gatgctggta aaaaattagg ggaatacact aaaaaataca
    19321 tagatgaaaa attaggtgga aagtcagaaa tagcaatagt aacagattta aaatctcaaa
    19381 tacaaatgca gagaatagat ggatttaaag atgcattaaa aggttcagca aatgttaaga
    19441 tattaaattc acaaccagga tatgacagag aagaatctct taatacagta gaaaacttaa
    19501 ttcaatctaa tccagatgta gatataatat atgcaacagc tgaaaatagt gtactaggag
    19561 ctaaagctgc acttgaatct gcaaaaaata aggatgttaa gattgtagga tttgacttaa
    19621 ctgaagaagc atcaagtgga ataacagatg gaactattct tgctatgatt cagcaacagc
    19681 ctaaagagat gggacgttta gctgtagaag cagtagtaaa agctataaaa ggggaaaagg
    19741 tagaaaaaaa tataccagtc ccagctttac tttatgataa ggaaaatata aaagacttta
    19801 agaattagca aaaaaaatga gattatttga aagaaatcct atttaaaaag gagtgtcatt
    19861 atgagtaata taattctgaa gttaagcaat attgctaaag aatttcctgg tgtcaaagca
    19921 ttagataatg ttaattttga gttatttcat ggcgaagttc atgcactact gggtgaaaat
    19981 ggtgctggta aatcaacaat gataaagatt ttaactggtg cacattcgaa aacttctgga
    20041 aaattcattt ttcaagataa agaaatagag aatatatccc cagatgtctc taaaaagata
    20101 ggtataaacg ccatatatca agaattaaca gtctttgatg aattaacagt agcacaaaat
    20161 atttttatgg gaaaggaaat aaatggcaaa gtattaacca atgacaaaaa aatgaatgaa
    20221 gaggctaaaa agattttcga taatatggga atagatgtaa atccaaatag tctagtcaaa
    20281 gagcttagta tagctcaaaa acaaatggta gaaatagcaa gagttttatc atcagaaact
    20341 aaagtattaa taatggatga acctacttct tcaataagca aaaaagagac tgaaatactt
    20401 tttagactta taaaggacct aaaagatagt ggagttagta taatatatat ctctcatagg
    20461 atggaagaac tttttgagat ttgtgacaga ataactataa tgcgagatgg gaaaacggtt
    20521 tcgactttga ataccaaaga tgtatcatct gaggaagaat tagttaattt gatgatagat
    20581 agaaagttag accaattttt tccgaagaga aaagtagaaa taaaagaaga aattatgaga
    20641 gtggaaaact taactaagaa caatgttttt aatgatgtta gttttaatat aagaaaaggc
    20701 gagatattgg gaataggtgg actagttgga agtaagagaa gtgagatagt agaagctata
    20761 tttggactta gaacatatga ttctgggaaa atatatttaa acaatgaaga agtaaagttt
    20821 aaaactccat cagatgcaat agaaaatgga ctaggattga taacagaaga tagaaaaggt
    20881 actggattgt ttttacaaat gagtgtcaaa gaaaacacaa ctatggctgg gttaaaaaag
    20941 atatctaagt ttaaatcaat tatagataga aaaaaggaaa aagagatttt agaaaaatac
    21001 atagaagctt taaggataaa aacaccacat atgaatcagg ttatacaaag tttaagtggt
    21061 ggaaaccaac aaaaagctat aatagcaagg tggcttttat tacaaccaga tatattaatt
    21121 atggatgaac caaccagagg tatagatgta aatgctaagg cagagatata caatctaatg
    21181 ggaaatttag tagagagtgg agtaagtata ataatgatat cttctgaaat accagaatta
    21241 atatcaatga gtgacagaat aatggttatg agagaaggtc atataagtgg atttttagaa
    21301 ggtgaagaaa tggttgaaaa taatgtatta aaattggctt tcggaggtaa aattaatgag
    21361 ttcaataact aagcataaag aagaaaaatc ttctccatta ttgacaaaat taacaagtgc
    21421 atttagagac caaggaagtg cagctgtagg acttgtaata atttttataa taatgagtat
    21481 agcttcatct aactttttaa ctttagataa tttaattaat gttggaagac aaatatcaat
    21541 aaatgcaata cttgcagttg gtatgacatt tgtaataatc acaggaggaa tagatttaag
    21601 tgtaggagca gtaattgctt tagtaggtac attttgggct acaacagtag tgaattataa
    21661 tgcacctata tggttaggta tgatacttgc attagcaata gggacaatac taggagtcat
    21721 aaagggtgca attatttcaa cacaaaaact accaccattc attgtaactt tagccatgtt
    21781 aacgataata agtggggcaa gttttgtatt tacaggagga agacctatat cagtaaatac
    21841 agatgcattt aagatgctag gtagaggata tgttggacca ataccaatac cagttataat
    21901 tatgattata gttgtaatag caggacattt tctgttaaaa agaactaatt ttggacgtca
    21961 tgtacatgca gtaggtggaa atgaagaggc tgctagatta tgtggagtga aagtaaataa
    22021 agttatagta aaagtatacg cactagctgg actactaaca gcattagctg ggataatact
    22081 atcatcacgt ttggcatcag gttcaccaac tgttggagat ggagcagaat tggatgctat
    22141 agcagccgtt gtgcttggtg gtactaatat gatgggtggt tctggttcta tagtaggtac
    22201 atgtataggt gttgggataa taggaatatt aggaaatgga ttaaacttat taagtgtatc
    22261 ttcatataat caaatgatta taaaaggttt ggtaatgtta tttgctatat ggataaataa
    22321 tataaaatta aaaaaattag ctaaatctaa atagcaatta aattttagtt tataaactat
    22381 aaaaattatt aagtttttgt atatattttt atactttaat ttgcatatat ctttatacat
    22441 taaatgggaa tatgctaagt gaaaaagttt actatggctt aaatagatga gattagactt
    22501 gaaattttaa atgtaaaaat aaatcttagg aggaaatatg aaaaaaattc tcttagatac
    22561 attaaatagt aaaaaacaag aatatataga ttatttaaaa gaacttgtaa gtataaagac
    22621 tgaagatgtt ggtcatggaa tactaggtgg atttgaaaaa gaaggacagg aatatattga
    22681 aaaacttgca aattacatag gttttagtgt agatagacaa gaaatgagcg aagaattaat
    22741 taaaaaagca aaaaatatat ataaagaagg aaatctaggt cacaattatc aagatagata
    22801 taatttgatt tgtaagtata gtgatgattt acctggaaag acaattgtat ttaatggtca
    22861 tgtagacact atgccaccag gtgatataag taagtggaaa tataatccat atcgtgctac
    22921 agaagataat ggaaaacttt atggtcttgg aattgctgat atgaaatctg gacttatagc
    22981 atcaatatta gctgtaaagc ttataaaaga tagtggtcta aatgtgcctg gtaatgtaaa
    23041 aataatgtca gtagtagatg aagaaggtgg aggaaatggt accataaatg cagttatgaa
    23101 tggtattgat ggagattgtt gcataatttg tgaaccatca gaacataact taatagttgc
    23161 acatatggga tttgtattct ttgaagttga agttaaaggt gtgtctcttc actgtggcag
    23221 taagtgggag ggtataaatg caattgagaa ggctatgttg ttgcttcaag atataaaaga
    23281 gttggagcat aactggctta tgatatataa gcatccacta ttaccttcac ctacaataaa
    23341 tttaggtgta ataaatggtg gaacagcagg ctctactgtg ccagataagt gtgtatttaa
    23401 cttatgtgta catttcttgc caaatataat gagttatgaa caagttgtaa atgatgtaac
    23461 aaatgtcata atgacaagag ctaatggaga tttgtggctt aaagataata aaccaaatat
    23521 aaacatatat caatcagggt taggctttga gatggacaag gattctgatt ttgtagtgaa
    23581 tgtacataaa atactagaag aaactctagg gagaagctta gaaataaaag gttcgacagc
    23641 tggcaatgat gcgagactaa tgaaaaattt agctgaaata ccaacactta tattaggacc
    23701 aggttcaata gaacaatgtc actcaattga tgaatatgtt gaaattaagg agtatttaga
    23761 tagtatactt atgtatgcaa gtctaatatt aaacttataa aagtaggagg aatatataaa
    23821 tgaaaaaagt attaatagta ggggaatctt gggtaaaaaa tataactcat ataaaaggat
    23881 ttgatacatt tgttacaact cattatgaag aagctgtaaa gtggttgaaa gaagcaatag
    23941 aatctggtgg atatgagaca gttcatatgc ctgcacatgt agcagcagat agttttcctt
    24001 ataaattaga agagttaaat gagtatgact gtataatatt atctgatata ggttctaata
    24061 cgttcttatt atctaatagt acttttatag attacaatag caatccagat agacttgaac
    24121 taatcaaaga gtatgtaaat aatggtgggg cattaatcat ggtaggtgga tatatgtcat
    24181 ttacaggaat agatgcaaaa gctagatttg gagaaactgc tatcaaagat gtattaccaa
    24241 taacaatgat agataaagat gatagagttg aaaaaccagc aggaataatt ccagaagttg
    24301 ttgactctga acatccagta ttaaaaggta taccaacaga atggccgaag tttttaggat
    24361 ataacaaaac agttgctaga gataattgtc ctgtgttagc aactattgga ggagacccat
    24421 ttgtagctgt tggtgagttt gggaaaggga aatctgctat ctttagttct gattgtgcac
    24481 ctcattgggg tccaaaagaa tttactgatt ggaaatacta taataaatta tgggttaata
    24541 tgcttgactg gttaacatgt taaaaatttt ataaaacttg aaaacctctt acttgataaa
    24601 atgaaattta caagttaaga ggttttattg cataaaatgg ctgtaaagta gaaaatattt
    24661 ttaaatcgtt aaaattagtt attttggcta atgaatggat aagttattag aaataaggag
    24721 gtttatacaa ttaatatgag tgatttaaat aaaaaatttt taatagaata tatagatgag
    24781 atacaggaag agttgagaga tatatcagat aaaatatgga aaaatccaga acttcagtat
    24841 aaagaatatt atgcttcaaa cttacaaaaa gagtatctaa aaaaacatga ttttagtata
    24901 gatgaattag agggtatgga tacagcattt gtggctagtt atggagaagg tgaaccagtt
    24961 atagcagttt tgggagaata tgatgcgtta gatggtttgt ctcaaaaagt atccattact
    25021 aaagaaccta ttttagatag tggtgcgggt catggatgtg gacataattt acttggtaca
    25081 ggttcaatag gggctgtaat tgcagtaaag gaattaatca aactaaaaaa attgaaagga
    25141 actataaaat actatggatg tccagcagaa gaggatttgt caggaaaagt actgatgata
    25201 aaaaaaggtt tttttgatgg aattgattgt gcttttagtt ggcatccatt tgatataaat
    25261 actccaataa gaatcccaac acttgctaac tattctgtga aatttagata caatgggatt
    25321 agtgcccatg cagcacaagc tccttataat ggaagaagtg cacttgatgc agtggaactt
    25381 atgaacatag gttgtaatta tttgagagag catatatttg atagctgtag agtacattat
    25441 gtaactacta atggtgggaa aatgccaaat atagttccag attttgcaga agtatggtat
    25501 tacataagag gaattaaaat ggagcatgta agagatgtat ttgacagaat tgttgatata
    25561 gcaaaaggag catcattaat gactggtact agtatggaat atgacattat aagtggagta
    25621 tatgattata ttcccaatac aatattaacc gatatcctat cacaaaatat gaaattgata
    25681 ggagtgcaaa actataatga agatgattac cattttgcag aaaaattagc tgaaacggtg
    25741 tctatagata aaagaattag tgtttctagt gtgttaagtg gaaataagga tattactaag
    25801 atgaatttac atggtgaagt tacagatgat acttttactc ataataactg tatatcatta
    25861 tcattggata ttggagatgt tagttacata attcctacag cacaatgttc ttgttcggtt
    25921 tggcctattg gtatttctgc tcatacatgg cagtcttgtg ccagtgcagg ttctgatatg
    25981 ggatttaaag ctatgttgtt agcatctaaa tctatagcat gttcaataaa tgatgtgatg
    26041 cttgatgagg tgattataaa taaagcaaaa aaagaattga agaatactat aggtagtttt
    26101 gaatatatac caattattta aaacttaaat tgataatata agcatgaaca acttatataa
    26161 gtatataatt taatattatt tttaacttaa ccttctaatt attttaaatt taagatacaa
    26221 tgattagaag gaatttttta agttttttag aatactgtat cttatataat cttgataaaa
    26281 agtgttatac tatatataat ctaaataaaa aatagtacaa aaaggagtat ttatgtttgg
    26341 atatgttaaa ataaacaaaa tggatttaac atttagagaa tatgactatt acaaggctta
    26401 ctattgtgga ctatgtaaat acttaaaaag aaatcatgga gaaatctcta ggttttcttt
    26461 gaattatgat ataacatttt taattgtttt attaacagca gtatataacc cagagtctat
    26521 aagcacagaa gaagtatgta tagtaaatcc atttaagaag aaaaaggtaa ttacaaatga
    26581 tattacagaa tatgcagcaa gtatgaatat tcttttaacg tattataaat tagaagataa
    26641 tttaatggat gatagacgca taaaagataa actggcatat tatatatata aaaataaatt
    26701 aaaattagca tatgaaaagt atcctgaaaa agcagagtat ataaaacaac aattaaatga
    26761 attaaataaa ttagaaaaag acaaaaatat aaatatagat gaagtatcta gtatatttgg
    26821 aaatataatg ggtgaagtat ttgcctataa aaaagatgaa aatgagagaa acttaagaat
    26881 gataggtttt aatataggta agtatatata tctattagat gcctatgaag atttagatga
    26941 agattttaag aaaggaagat ataatccatt tatagagtat attgataaaa atgatgagtt
    27001 aaaagaaaaa gtaaaaaaaa taatagtgac atcattaggg tttttagcta ggggaattga
    27061 taatctaaat ctaaataaaa atgttggaat aatagaaaat ataatttatt ctggtgttta
    27121 tttaagatat atgaatatat tagaaagcag aggtggaaag aatgtacagt aatacttata
    27181 ataatgaaca ttttatgaga gcaagagcct tcatagaaaa taatgaattt aaaaaagcat
    27241 atgatttttt aaaaactttg actgacaaat gtgcagagtg gtattattta actggttttt
    27301 ctgcaatgaa tataggatat tatgaagaag gtgaagactt tttaaaaaga gcaaaattta
    27361 tggaacctga aaatagtgaa tatagtgatg cacttagaag ttatacacaa tatagaaatg
    27421 attatagcaa tagggcagac aattataata gaagaagacg taatgattta gatggatgtt
    27481 gttgctgttg ctgtgatgat tgctgttgtt gcctaggtga cgattgttgt gaaaattgtg
    27541 ctaaactttg gtgtttagat tcatgttgtg aatgttttgg tggagattta ataacttgtt
    27601 gttaggatga taaatatgag tataaaagtt gcttatggag gagttttatt agcattaaat
    27661 gtaatattgt tgactcttac aaatattatt cctgtaaata cattgtttat tatggggtta
    27721 gcatcacttt tagtatcaat tgtaataatg gaatggggct ttaagagtgg aattgctttt
    27781 tatataggtt ctatagcttt aggatttata gtgatggcaa gtaagtctca atggattgta
    27841 tattcactta cctttggaat atatggtatt gtaaaatatc ttatagaaaa agataggtct
    27901 atatatattg agtattttat gaaattagta tttgcaaata taatgatact tatattgtat
    27961 tttttattaa gaacaatagt ttatattcca ataaacatat ttatcatagg ttcatttgag
    28021 atagccttta tagtatatga ctatgttttc agttctttta taggttatta caataatagg
    28081 ttaagaaaga tgctatttaa aaaataatag ttgattttta tatttaattt taatggaatt
    28141 gtagtttgga taagtgtaaa tgatatatct gaatatacat taaaacataa agctaaattt
    28201 aatatattct tagtgtaact aataaaagct ttgattttta gtcaaagctt ttatttatta
    28261 aatataaata ttacttataa tataagtctt taaaatagat agtataaatt aaatatccac
    28321 tatatttaca tctgtagata aagaataaat aatatctttt ctttctacta ttatttcaac
    28381 atttttagaa atttcctcat caagtgatat ttgagacaaa agctcctttg ttggatttat
    28441 tgctattgga tttccaactc tctttaacat atttatatct ccattggtat ctccataggc
    28501 atacgagttt gataaatcta agtcgtactt tacaacaaaa tcatttatag cattattctt
    28561 acttatagaa tcccacattg gaataactgt gcctgtaaat atattatttt caaagacata
    28621 atcacttcct agaaaatcgg ttacattata tttttttgcc attttttcaa caagaaatcc
    28681 agggctacca gatataaata taactatatg tccttgattt agatgccact caatccttga
    28741 acgcgtgtat ttataaactc tatctgcttt taatttaatt acttggtcac ttgtaaagtt
    28801 gatacaggtt ttatctaatc cttttaatga atcaacataa aggtcacata tttctaatag
    28861 ataatcatca taattaccct gtcttttatc ccaatccata aaagtatctc tagcatgttt
    28921 ataccaattt ttttggtcaa ttatatcgta tttaattaat ttcttaaaat gttcaaccat
    28981 aagagagtct ctataaagag taccatctat atcaaaaaaa gctgctatat ttttcataag
    29041 accacctcaa ttaatcattt ccttatatta tattataaat tgtatctaag ttgttaaaat
    29101 atatgtataa taatttatat attaaaaaac aagtatttta aagtttaaag gatgtgacta
    29161 tatgaatttt aattttgcaa aggctacaaa ttctagactt atgggcagtt taggtcttat
    29221 gataaattgg atagatgatg aaaacaatca cttttgtcaa tattttttat tagatgcaga
    29281 gggattagga ttagcagatt atgtgagttt aaaaaatccg acgcaagaag aagcttatat
    29341 ggaagaagaa aggttgatgg gaggatttgg ctcagataga gttgaattaa caaaggatga
    29401 atctttattt ttagtatctt attttggtaa taagaatctt tattataata agctactgcc
    29461 aggagataaa tgtgaatata ttgatattat aaaaaactat aaaacagatt taaccataga
    29521 aaaattatat gataaaatat gtaaaagagt tgatgaggaa gttgaattta taaactatat
    29581 gactatgaga tttatagcat gggatagaga aagtttaaag tacttttctg gaagtgacga
    29641 gattgcaaat atgcatatta ccaatataaa tggaacatta cttaaaaata tagtaagtga
    29701 taaaggtcaa ggcagatata taagtgaagc tttatatgaa gacaatgatg gatattatat
    29761 atgtaaaatt gctttttgta taagtaaatg ttatgaaact ggttttagaa ttaattctct
    29821 attagtaaca gataaagaac ctatgtatga ttttgaggta tttgatgaaa tttctaaaag
    29881 cgaatttgta agtgtataca gtgtaaattc gcctgaagag tttgcaggag tattttatag
    29941 agaaaatcca tttcttttaa aaagtgatat gaatgaaggt atatttttta ctagatttaa
    30001 ttttaataat gaccatgtaa aagaaaatgt atatgtaata aataatgata tgaaagctat
    30061 atattactta atgggaaata agttttttat tggtacttat gacgaaaatg attgtaatta
    30121 tatcaatgaa ataagtcagt ctaattatag tgactatatt aaatttgaag agaatttttt
    30181 ctttgagcaa aatgcattat atgattttgc agaatcaggt tcattagatt ttgatgactt
    30241 tttggaataa taaatttagg cattatttaa gcgagattaa ctgaatttaa tctcgcttaa
    30301 atttatttc
//
LOCUS       DAFNNB010000031        28640 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0031, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000031 DAFNNB010000000
VERSION     DAFNNB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28640
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0031"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            146..2002
                     /locus_tag="L3M60_001229"
     CDS             146..2002
                     /locus_tag="L3M60_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HBN6225916.1"
                     /translation="MDTHNKYVNFIKNIPVPFLYCRIVKRQEDIEYQVEYISKGMGKV
                     LQLEEGICDKNILDVLPVFKSKKYFKELFSNEVDCIKRYIPTLKNWINIKKQIVGDSY
                     IILYFGKIVFDYRQIIDSFDKKEKVAYIKDEEGIYIDCSENLIPILNNNIKTTKDIFG
                     KNDIEVWGESTGKLFKDDYREGVSSKKRFLQNLFEYEETFFMVEKYFLYDEDELLGTI
                     GIIDNIIYSGYSNKNYNSKDLMKMIEHSIPENMFYKDVYGNYIGFNSGFLNLACMNKE
                     ELLGKNSYKISEEEALIDKIFESDKGVVESKKVVTFELNISMNDENKCIEITKRPFFD
                     SYGSVIGIIGTAKDISRRKRLEEEMDKTRMEFFANLSHELRTPINLISSSLQVIEKKE
                     ADLIESNDTLKRNLGIIKQNGNRILRLVNNVIDFTKIQSGYLDFKPEESDIIAFIEEI
                     CMSVADFASQNNIQLTFDTEIEEFSMLFDSEKLERIILNLLSNGIKYNKEDGKINIFL
                     YVKDNVFNMKISDSGIGIPKEKIDKIFNRFEQIDNELSYRVKGSGIGLSLVKSLVELH
                     EGSISLKSQLGVGSEFIVSLPVRSKNNIEKYNHKREISNELSKKLEIEFSDL"
     misc_binding    2085..2325
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    2391..2626
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            2711..3928
                     /locus_tag="L3M60_001230"
     CDS             2711..3928
                     /locus_tag="L3M60_001230"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HBN6225917.1"
                     /translation="MENKVKIGVLGYGVVGSGLIDIIDNNKEKRNIEIVGILVNNLEK
                     HKGKKYSNMITNNIDDIFNKDIDILVEVMGGLEPALSYIKKALNNKIHVVTANKDLLA
                     ECGDELAKLASENKVSIKFEASVAGGIPVLKPIIESLEGNNIDSINAILNGTTNFILS
                     KMYDENLSYDMALRQAQELGFAEANPESDVLGYDAARKLSILSTLAYDNRVYWKDVYL
                     EGITDIDEKDIEYAKKLNCKIKLIGQSKYENDKVSAFVRPVLVEKDNILARIDNEFNA
                     VIVNGDSVGEVSFVGKGAGSLATGSAVYSDVIDIIDNRVSSIDSFTKDKIQVNKIVRE
                     KCGALLRFKKCNKDEILNIVENCLVKFDILNDDDELAIMVYADSEYEINNSLCLIKDK
                     GYCEKMNKMLKIS"
     gene            4044..4538
                     /locus_tag="L3M60_001231"
     CDS             4044..4538
                     /locus_tag="L3M60_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225918.1"
                     /translation="MENKKYANGGYSEDWYERGESTAKWFQNDREEYERAAYDEDRER
                     RGSNCGGSDSEGNRPRNCERFRREAEIREREAREAFCESSERKKEALAYECEARKLWE
                     QAEKYWDEYSKYNYQGIEYLAEAARLFDEGMECEARRNGNNGGNNNNCCHKCHKCNCN
                     CCRK"
     gene            4777..6042
                     /gene="hisD"
                     /locus_tag="L3M60_001232"
     CDS             4777..6042
                     /gene="hisD"
                     /locus_tag="L3M60_001232"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBN6225919.1"
                     /translation="MKRIKTSSMKEKVDEKILREQVSSIIEDIRNNKDIALKKYNEKF
                     DRNTRDEFRITKEEIKEAYKHVDDEFINNLKIAAKNIKEFAKAQKSSFENSFEKEIYP
                     GVILGQTNIPIESCLAYVPGGQYPLFSTALMLIIPAKVAGVKRIVACSPTMKNTEKIN
                     PKTLVAMDIAGADEIYATGGVQAIAAFTYGTEKIKPVDIIVGPGNKFVTEAKRQCYGQ
                     VGIDFVAGPSEVLIIADETSKPVYIAADLLAQCEHDLNARGILLTNSLEIAEEVEKNI
                     ETMLKDLPTKDIAYSSWKNNGEIILVDDMEEAIKISNFYAPEHLEIAVNKCDDIFDRL
                     TNYGSLFIGNLSAEVFGDYVSGTNHTLPTLKASRYTGGVWVGTFIKTCTKQIFNEEAI
                     ESLAPVAEKLAKEEGLYAHAKAAEVRFKK"
     gene            complement(6247..6999)
                     /locus_tag="L3M60_001233"
     CDS             complement(6247..6999)
                     /locus_tag="L3M60_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6225920.1"
                     /translation="MSYFQYQGNACFYEEYGQGKPIVFLHGNTASSNMFKALMPLYVE
                     NFRCILIDFLGNGQSDRVSQFSPDMWHDEALQTIALIEHLNCGKVGLIGTSGGAWAAV
                     NTGLERPDLVEAIIADSFDGRTLNDNFIDNLITGREKSKQDIQARKFYEWCQGKDWEN
                     VVNLDTKALLQCAKEKRPLFHKELCKLEMPILFIGSKEDEMCRHNLEEEYKQMATFIR
                     KASIHIFSHGGHPAILTNADEFAQLAKVFFKY"
     gene            complement(7117..7386)
                     /locus_tag="L3M60_001234"
                     /pseudo
     CDS             complement(7117..7386)
                     /locus_tag="L3M60_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436543.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7861..8635
                     /locus_tag="L3M60_001235"
                     /pseudo
     CDS             7861..8635
                     /locus_tag="L3M60_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429999.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            8630..8835
                     /locus_tag="L3M60_001236"
                     /pseudo
     CDS             8630..8835
                     /locus_tag="L3M60_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861264.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
     gene            9241..9873
                     /locus_tag="L3M60_001237"
     CDS             9241..9873
                     /locus_tag="L3M60_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBN6225921.1"
                     /translation="MARRLFLERIGLIFLGSAILAFGVYNFYYLNNITEGGVLGILLL
                     LKNLFNIQPAIANVVIDGLLLLVGYKFFGKKFLIYSIVASITFSVLYDLFEAIGPLVP
                     QSQNMLLSTILAGVTVGTGVGIVVKAGCASGGDDALALVISKTTSLNIGQVYLATDVI
                     VLLLSLFYLSAFDIFYSLIAVTISGKVIDFIYYHGKSLDMDISNDIVPEC"
     gene            10284..10376
                     /gene="kdpF"
                     /locus_tag="L3M60_001238"
     CDS             10284..10376
                     /gene="kdpF"
                     /locus_tag="L3M60_001238"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBN6225922.1"
                     /translation="MEGLPMWFLAVVIIMLIIYLVYALINPEKF"
     gene            10417..12099
                     /gene="kdpA"
                     /locus_tag="L3M60_001239"
     CDS             10417..12099
                     /gene="kdpA"
                     /locus_tag="L3M60_001239"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015391009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBN6225923.1"
                     /translation="MFMVQIVIVLAIFMILVIPMGKYLYHIATNQKTFGDRLFDKVDN
                     FIYKVCSIDKKKEMNWKQYALALLFTNAVMVFIGYIILRTQSMHIFNPSGIKSMEQGL
                     SFNTIISFMTNTNLQHYSGESGLSYFSQMTVIIYMMFTSAATGYSAAMAFVRGLVGKK
                     KTLGNFYVDLIRITTRVLLPGALIIGIILVTQGVPQTFAGTETVTTIEGKLQDIARGP
                     VAALESIKHLGTNGGGFFGSNSSHPFENPTIISNIVEILSMMILPGACVVAFGYMIKN
                     KKQGWVVFGAMSIIFLIGLVVCFKAESAGNPILSQLGLNQSMGSMEGKEVRFGIAQSS
                     LFTAVTTSFTTGTVNNMHDTLTPLGGLVPLLNMMLNVVFGGKGVGLMNMLMYAIIAVF
                     LCGLMVGRTPEFLTKKIEGKEMKLIALLIILHPLLILMFSGLSVAVPAGLEGISNPGF
                     HGLSQVLYEFSSSAANNGSGFEGLGDNTMFWNMTTGIVMFFGRYVSIIVLLAISSLLA
                     SKKAVNESIGTLRTDNFTFTIVLVLVVLIVGALTFFPALALGPISEHLVLWH"
     gene            12124..14181
                     /gene="kdpB"
                     /locus_tag="L3M60_001240"
     CDS             12124..14181
                     /gene="kdpB"
                     /locus_tag="L3M60_001240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBN6225924.1"
                     /translation="MDTKKTKFITKDIFKTSIIEAFKKLNPKYMMKNPVMFVVEIGFI
                     VTLILTIVPSLFGDEGNNLRVYNGIVTIILFITVLFANFAEAVAEGRGKAQADTLKKT
                     RKDTVARVIDTLGNEKMISASELKLGDIVLVNAGEVIPNDGEVIEGIASVDESAITGE
                     SAPVMRESGGDFASVTGGTTVVSDWLKIRITTKPGESFLDKMIALVEGASRQKTPNEI
                     ALNTLLVGLTLIFMVVLISLYPMANYAGVKIPISTMIALLVCLIPTTIGGLLSAIGIA
                     GMDRVTRFNVIAMSGKAVEACGDVDTMILDKTGTITYGNRLASEFITVGNADSKDLIN
                     YSVMCSLKDDTPEGKSIVDLGMKLGSTGKTKEAEEAEFVEFSAQTRMSGINLADGRAI
                     RKGAYDSIIKRVKESGGIVPEDLEENVNRVAKLGGTPLVVCVDNGIYGIIYLKDTVKS
                     GLVERFARLREIGIKTIMCTGDNPLTAATIAREAGVDGFIAECKPEDKIDAIKKEQSE
                     GKIVAMTGDGTNDAPALAQADVGLAMNSGTTSAKEAANMVDLDSDPTKILEVVEIGKQ
                     LLITRGALTTFSIANDVAKYFAIIPAIFTLAIPQMEILNVMKLSTSFSAILSALIFNA
                     IIIPCLIPLAMKGVKYRPMKSQAMLMRNMLIFGLGGVIVPFLGIKLIDLLITPLVMVL
                     NLG"
     gene            14200..14826
                     /locus_tag="L3M60_001241"
     CDS             14200..14826
                     /locus_tag="L3M60_001241"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBN6225925.1"
                     /translation="MKTLKPALLVSIVLLVVCGLVYPLVLTGVSQVAFKDKANGSMIE
                     VNGVKVGSELIGQSFTDARFFKGRVSSVNYNTYTKEDLVPDKDGNTSYGGVSSGSFNY
                     GATNPELRDRVQKDIEQFLKDNPTVKKEDIPTDLLTASGSGLDPNISPDSAKIQIPAV
                     AKASGISESKLQKIVDDNTSKKVFGVLGEDRVNVLKVNVEVARILGLI"
     gene            15063..15971
                     /gene="cysK"
                     /locus_tag="L3M60_001242"
     CDS             15063..15971
                     /gene="cysK"
                     /locus_tag="L3M60_001242"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBN6225926.1"
                     /translation="MLYNNALELIGNTPVVRLNNLGCTNGCSNIYVKLEKVNPAGSIK
                     DRAVYEMLEGLEKRGELKKGDVLVEATSGNTGIALSMIGKLKGYEVVIVMPETMSIER
                     RDLMKAYGANLVLTDGKAGMAGSIEKANELLKENPNYKSLKQFENKDNPNAHYKTTAV
                     EIMNDVKDLDIFVCGVGTGGTLIGTARYLKEQNPNIKVVALEPKKSPAISENKTGPHK
                     IQGIGANFVPENYDSSVVDEIILVDDEDAFDTVKLLASKEGILVGISSGANIFGAIEM
                     AKRYPDKKIVTVAPDGVDKYMSMGIF"
     gene            15998..16588
                     /gene="cysE"
                     /locus_tag="L3M60_001243"
     CDS             15998..16588
                     /gene="cysE"
                     /locus_tag="L3M60_001243"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBN6225927.1"
                     /translation="MFKKINKDIEYIMKNDPAARSKIEVFLLYPSVHAMIMHRMAHAL
                     YKKKKLFTARLISQISRFMTGIEIHPGAKMGEGILIDHGMGVVIGETAEVGNRVTIYQ
                     GATLGATGKDTGKRHPTVGDDVLIGAGTKILGPLNIGSNSKIGANSVVVKDVPNGATV
                     VGIPAKIVKIRNLEPVKKNKKEVDYEYDELDNVYYI"
     gene            16749..16937
                     /locus_tag="L3M60_001244"
     CDS             16749..16937
                     /locus_tag="L3M60_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBN6225928.1"
                     /translation="MKANVNQDTCIGCGLCPSICPEVFDMNDDGKSHVIVDEVPSDAE
                     ESAAEARESCPVDAIDVH"
     gene            complement(16991..17860)
                     /locus_tag="L3M60_001245"
     CDS             complement(16991..17860)
                     /locus_tag="L3M60_001245"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HBN6225929.1"
                     /translation="MKLNRNDSCWCGSTKKYKKCHMDMDNKIDSFAKLGHIVPTHDII
                     KNESQIEEIKKSAVINTKILDYISDKIEVGISTEDINKLVHEFTLQNGAIPAPLGYNG
                     FPKSVCTSINNEVCHGIPNKDIILKDGDIINVDVSTIFNGYYSDASRMFMVGNVNENA
                     KKLVQVTKECVQKGLEVAKPWGFLGDIGDAVNSYAKKNGYSVVREIGGHGVGLSFHED
                     PFVSYVSKKGTEMLLVPGMIFTIEPMVNEGTHEIFVDEENNWTIYTKDGKLSAQWECM
                     VLVTETGVEILTE"
     gene            complement(18057..19307)
                     /locus_tag="L3M60_001246"
     CDS             complement(18057..19307)
                     /locus_tag="L3M60_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225930.1"
                     /translation="MIMENNLNTAKRNNTELSQNDSLKVNLQIGVDFTLLSKSLKAFY
                     KKEKDFYCIALAPCNVSSEEANRKISIYDMIKEINILIESITDKNELLSEELFTNNLS
                     EFYDESLDEIYIDLKQAYLYIKKNTNGLNIEDSIEYVFDVDISNDIKAKENPLINFNS
                     LSLVVWSGETKHILQQMNILNLEDIHSYSMENNTNITIYENDNLQYKFDEESEDIKDS
                     NIDIIEQMLILPDDLNYDKDEVENMKSRLAKINIKYLQALKEKDIKIKLINSNLTDEP
                     EFSDLKYQLPPCWVRSGKTWKDVPGIYRNNSIVAKIGYSNPSYTNVHSSKNLELHETA
                     HAIDKNVLNKKSNSEEFMKVFAQERYKLYDPKQIAHAYISKFIEEFFAESFVHYYLDE
                     DSKNTLKENCPLTYDFLEKLELYY"
     gene            19594..19896
                     /locus_tag="L3M60_001247"
     CDS             19594..19896
                     /locus_tag="L3M60_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBN6225931.1"
                     /translation="MYFTYIIRCKDDSLYTGYTSNIVRRMNEHKLGVNSKYTRAKGFK
                     KLEVYFVTNTKSNAMKLEYYIKKLTRNKKLSIIKNPNILINSINNKTDYIIGKEIE"
     regulatory      19998..20090
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20190..20984
                     /gene="thiD"
                     /locus_tag="L3M60_001248"
     CDS             20190..20984
                     /gene="thiD"
                     /locus_tag="L3M60_001248"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBN6225932.1"
                     /translation="MSNYKIPTLTIAGSDSSGGAGIQADLKTFSAIGTYGMSVITAIT
                     AQNTQGVFAVEDLNKNIIKKQIEAVFEDIPPRAVKIGMVSSPEIILEIVKNLKKYNPK
                     YLVVDPVMISKSGYYLLKPEAKENLIKYLIPLAYIITPNIPEAEEITGIKIHNVDDMK
                     RVGKEILQLGPKFVLMKGGHLDGEAVDILIGKNIFKVYKSERIDKKNTHGTGCTLSSA
                     ITSYLALGYEITEAVNFSKIYITEAIKRSFDIGHGVGPVHHFYKFE"
     gene            21008..21793
                     /gene="thiM"
                     /locus_tag="L3M60_001249"
     CDS             21008..21793
                     /gene="thiM"
                     /locus_tag="L3M60_001249"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBN6225933.1"
                     /translation="MYNLIKDVKKLNPLVIHYTNNVTINDCANATLAVGASPLMSFSY
                     EEVEEMVSVANSVVINIGTMNSNMLDLFLLAGKAANKYNKPVVLDPVGVFASKARAEL
                     TSRLLNEVKFSVVKGNVSEIKFIGGFNVRGKGVDSFDEEDSTDIIRKVAKKLECIVVA
                     TGKIDIITNGKSTYKINNGTDKLKGITGTGCMTASLIASFMAVTENILEAATMGVLTM
                     SLSGELANLNNPPIGTFKENLMNAIYQMDIDTLSKNSNIEFLN"
     gene            21816..22448
                     /gene="thiE"
                     /locus_tag="L3M60_001250"
     CDS             21816..22448
                     /gene="thiE"
                     /locus_tag="L3M60_001250"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBN6225934.1"
                     /translation="MIDKESLKKCLRLYLITDSEMLEGRDFYKCIEDAISSGITTVQL
                     REKNTCGREFLRKAMKLREITKRYGVKFIINDRVDIALICDADGVHVGQSDIDVREVR
                     KLIGNDKILGVSARTLEEAICAKNDGADYLGIGSIFSTSTKLDAKSASFETVKEIKEK
                     VDMPFVLIGGINLDNIDKLKCLDSDGYAIISAILKAEDISKEVEKWTLKI"
     gene            22587..22889
                     /locus_tag="L3M60_001251"
     CDS             22587..22889
                     /locus_tag="L3M60_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225935.1"
                     /translation="MIRILLVCVGGMSSTLLVNKMEKDAKKRNIDCKIWAVGEGDIKS
                     ELDNFDILLLGPQLRFMFDDVKSIVGDRAPVSIIDMVNYGTCNGHAVLNSVLEILK"
     gene            complement(22955..23704)
                     /locus_tag="L3M60_001252"
     CDS             complement(22955..23704)
                     /locus_tag="L3M60_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225936.1"
                     /translation="MLNILWRSMKWRFKNPISFIVTILQPFLWLVLYSSIANQTMNNI
                     NINNYTAFILPGIIILVVFSSCSSGGIINFIMKNSGSFYRVLIAPISRYSIVLGQLLE
                     AILVSFIEVAILCIVSIFFSVTIESGIGGILLMTVLVFMTAFFLSSLAYSISLLLPNE
                     IVYETIMTAIVLPIFFLSSALFPIENLSGGLKVVVMLNPFTHVINALRSLIFGETVLI
                     EDILPVILLFLILCCASFSLAMWRLKKEMVS"
     gene            complement(23698..24660)
                     /locus_tag="L3M60_001253"
     CDS             complement(23698..24660)
                     /locus_tag="L3M60_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225937.1"
                     /translation="MLAVEIDNLVKEYKNGVKALNGLSFNVNSGEIFSLLGPNGAGKS
                     SLINILTTFYKPTSGNVTMFGKDLVDNPSWIRTQIACVAQQISIDEHLSLMENMIFQS
                     KMYKVKPQIAKQRIDSLIDKFDLSSYLKYPTSSYSGGVKRRLDIAMNMVSSPKILFLD
                     EPTVGMDVDSRKSMWDMLLKIRYEYGTTIFLTTHYLEEAEQLSDNICIMKNGKDLAQG
                     TPSSLRSYIRQNIIRITFHNTEDIKKYNDAIKGTGLVKFMNVRENSIFISVNDSRTAF
                     TLINKWLLEHDIEFDAIEIVEPSLEDVFLALTSSKKSLKEGWEC"
     gene            complement(24664..25116)
                     /locus_tag="L3M60_001254"
     CDS             complement(24664..25116)
                     /locus_tag="L3M60_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBN6225938.1"
                     /translation="MSYKIKNIESVTVATMRYNGPMTEATKYFPNVFKAIKGKSNGAP
                     FFCYYDVDQKTGVAEMELCVPTAETPNRMGITTKEFPKTKALFFTHIGSYSNLPKTYE
                     MIFKYIHENNIKIQTPWREVFIKGPGMLVKGKPDNYITEIIFPLKEEE"
     gene            25477..25833
                     /locus_tag="L3M60_001255"
     CDS             25477..25833
                     /locus_tag="L3M60_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6225939.1"
                     /translation="MNFELDNTTPIYLQIVKYIKRQIVIGELKPGDTIPSRREMALNL
                     KVNLNTVQRAYKEMGDMNIINTFKNHQSSVTIDENILKNLKLELINESLSVFLKDMKA
                     INVTKEEVLKIIEDKY"
     gene            25905..26621
                     /locus_tag="L3M60_001256"
     CDS             25905..26621
                     /locus_tag="L3M60_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6225940.1"
                     /translation="MIEIKNVSKTYKRMQGLKIRKIEALKNVSFNIEKGKITALLGIN
                     GVGKSTMLKAIAGLINTDSGEIRIDGEKINEKIYNKLAFVPDVQSHFSNTTIKETFEF
                     MEIFYSKWNKKKSKEMMNIFKLDEDEIIDNLSKGNIARVKLILGFCQDPEYILLDEPF
                     TGIDLFKREEFIGVIAQYMEENQAIIITTHEIVEIESLVDEVIILDEGQIIASFNAEE
                     LREREGKSILDKMREVYKNE"
     gene            26614..27438
                     /locus_tag="L3M60_001257"
     CDS             26614..27438
                     /locus_tag="L3M60_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6225941.1"
                     /translation="MNKLLYLINVDLRRSNKFYIAYISFFSLITLGLNLFEINKFKNS
                     KFIINLAIGDFGGIFYGIGILSNLGFIQSILFFGMLGLFIYYIFMWKREFFSNNQSIY
                     TLMMLPQNKFKICISKSIALLTMIYGFLISQIATLFICKYIFNFIFRNVPIINMNFAK
                     DLYYLNLKGIPIDFISFMAIYVFLLLIAISIVNCCILFIFSYINKFYILLITLLIIVG
                     LYFSTIYISIITLFRKYYMAIGFQFNNLVAGLLSFLIIMFILNGISYLLIKKKISL"
     gene            27449..>28640
                     /locus_tag="L3M60_001258"
     CDS             27449..>28640
                     /locus_tag="L3M60_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225942.1"
                     /translation="MKLKSKISILIIFLTLAIYFSIGLLNNEDNLKIKTIKGDPNELD
                     YININFGYAFNGYVNSYYTINGSNKTKKTELIKLQKDAKNTNNLIDVSSGSDLIAHVG
                     FEMNDDSKDINFVIDEKKEDLEKGVFPSEGDDKAVEYEKLDMNDFTRTRYKVDKSSDN
                     YYANILSSKRYKKYLYVSVLYATSDSSKNNKEYSSTIDIIKINPENKKIQTIKSINLS
                     NYFSKKYQNIDNISVSYASKYEDKMYILVNVLNANTGDKSNSKFHLLEYNLEENKESI
                     MELKTDSKMALAKVKLEDNKLYMVMSNDYDNSSITELNLLTYNILDKSLDEDKYILDN
                     KEKRGIRSIEIDSNKIIVYTSRLNSTLSWDNKFMLYIINKNSKEIVYEGELQFNSSNS
                     MLEFE"
BASE COUNT        11085 a         3200 c         4854 g         9501 t
ORIGIN      
        1 ataattataa acaaataata aattacataa aattaaattt attcacaagt atatcttata
       61 aaatatgtta taattaatag aaaattgtca tataattact taagaagtat tattataaat
      121 aacattgaga ggtagtggtt agaatatgga tacccataat aaatatgtaa attttataaa
      181 aaatatacct gtaccttttt tatattgtag aattgtaaag agacaagaag atatagaata
      241 tcaagtagag tatataagta agggtatggg caaagtatta caattagaag aaggtatttg
      301 tgataaaaat atattagatg tattgcctgt atttaaatct aaaaaatatt tcaaagagtt
      361 gttctcaaat gaagtggatt gtataaaaag gtacattcca acacttaaaa actggataaa
      421 tataaaaaaa cagatagtag gggattcata cataattttg tattttggta aaattgtatt
      481 tgattataga cagataatcg attcttttga taaaaaggaa aaagtagctt atattaaaga
      541 tgaagaaggc atatatatag attgcagtga aaatttaata ccaatattaa ataataacat
      601 taaaacaaca aaagatatct ttggtaaaaa tgatatagaa gtatggggag agagtacagg
      661 aaaattattt aaagatgatt atagagaagg cgtatctagc aaaaaaagat ttttacaaaa
      721 tctttttgaa tatgaagaaa catttttcat ggttgaaaaa tattttttgt atgacgaaga
      781 tgaactttta ggtactatag ggataataga caatataata tatagtggtt acagtaataa
      841 aaattataac tcaaaagact taatgaagat gatagagcac tcaattccag agaatatgtt
      901 ttataaagat gtgtatggaa attatattgg atttaattca ggttttttaa atttagcttg
      961 tatgaataaa gaagaattat taggaaagaa tagttataaa atatcagaag aagaagcttt
     1021 aatagataag atatttgaaa gtgataaggg tgtagttgaa agtaaaaaag tagttacatt
     1081 tgagttaaat ataagtatga atgatgaaaa caagtgtatt gagattacaa aaaggccatt
     1141 ttttgatagc tatggaagtg ttattggaat aattggaaca gcaaaggata taagtagaag
     1201 aaaaagatta gaagaggaaa tggataaaac taggatggaa ttttttgcaa atttatccca
     1261 tgaacttaga acacctataa atttaatatc atcttcatta caagtgatag aaaaaaaaga
     1321 agcagactta atagaatcaa atgatacttt aaaaagaaat ctaggcataa taaaacaaaa
     1381 tggaaataga atacttaggt tagtaaataa tgttatagac tttacaaaaa ttcagtcagg
     1441 atatttagat tttaaaccag aagaatctga tataattgca tttatagaag agatatgtat
     1501 gtctgtagcc gactttgcaa gtcaaaataa tatacaactt acatttgata cagaaataga
     1561 agaattttca atgctatttg attcagaaaa attggaaaga ataatattaa atttactttc
     1621 taacggaatt aagtataata aggaagatgg aaaaataaac atatttttat atgtgaagga
     1681 taatgtattt aatatgaaaa tatctgatag tggtataggt ataccaaaag aaaaaataga
     1741 taaaatattt aataggtttg aacagattga caatgaactt tcctacagag ttaaaggaag
     1801 tggtattgga ctttcattag tgaaatcttt ggttgagttg catgagggaa gtatatcttt
     1861 aaaaagtcaa cttggtgttg gaagtgaatt tatagtttca ttacctgtta gaagtaaaaa
     1921 taatattgaa aaatacaatc ataaaagaga aatcagtaat gaactaagta aaaaattaga
     1981 aatagaattt tcggatttat aagtttaagt ataaaaaata aaattttaaa aaagtattga
     2041 caattgattt agaagggttt aaaataaatt acaacataat aaataaaaaa gcgatgaata
     2101 ggaagagtaa atctaattcg attgccagag agaagagtcc attggctgga agacttttta
     2161 aattagaggg attgaaaact acctaggagc tactttctga agttatctac atcagtagat
     2221 tttagtaaga atagtcgtta actgcgttaa agtgtaaagt gagttttcat gatatatgaa
     2281 aactaaatta ggtggaaccg cgggaataat actctcgtcc ttatgttata aggatgagag
     2341 ttttttgtta tttaaaaata taaacagtaa tcataataat tatagaaaat ataaaagcga
     2401 tgaataggaa gagtaaattt aattcgacta tcagagagga aagttcgcag gctgaaagac
     2461 tttctaagtt tgagaaattg aaaactacct aggagctgtg gtctgaagtt actttaaaaa
     2521 gttttagtaa gactaaccgt taactgcgtt aaagtgtaaa gtggatttta tcaattaatg
     2581 ataaaatcaa attgggtgga accgcgagta tagactcgtc cctttatata gggatgagtc
     2641 tatttttagt gcaataaaag agtgaaaata aaaaaaaatc ggaaaattat aaatttagga
     2701 ggaaaataaa atggagaata aagtgaaaat tggagtacta ggatatggag ttgtaggaag
     2761 tggacttata gatataatag ataacaataa agaaaaaaga aacatagaaa tagttgggat
     2821 tctagttaat aatttggaaa aacataaggg taaaaaatat tctaatatga taactaataa
     2881 tatagatgat atatttaata aagatataga tatacttgtt gaagttatgg gtggtttaga
     2941 accagcatta tcttatataa aaaaagcatt aaacaataaa attcatgttg ttacagcaaa
     3001 taaagatttg ttggctgaat gtggagatga gcttgcaaaa ttagcaagtg aaaataaagt
     3061 aagcattaaa tttgaagctt cagttgcagg aggtatacca gtattaaaac caataataga
     3121 atcactagaa ggaaataata tagatagtat aaatgcaatt ttaaatggaa ctactaattt
     3181 catattatct aaaatgtatg atgagaatct atcttatgat atggcgttaa gacaagctca
     3241 agaattaggt tttgctgaag caaatccaga gtcagatgta ttagggtatg atgctgctag
     3301 aaaactatca atattatcaa ctctagctta tgacaataga gtatattgga aagatgtgta
     3361 tttagaagga attacagata tagatgaaaa agatattgaa tacgcaaaaa aattaaattg
     3421 taaaataaaa ttgattggtc aaagtaagta tgaaaatgat aaagtaagtg cttttgttag
     3481 acctgtttta gtagaaaaag ataatatact tgctagaata gacaatgagt tcaatgctgt
     3541 catagtgaat ggagattcag taggagaagt ttcttttgta ggtaaaggag ctggaagttt
     3601 agcaacagga agtgcagttt actcagatgt tatagatata atagataata gagtttcaag
     3661 tatagattct tttactaaag ataaaataca agttaataaa atagttagag aaaagtgtgg
     3721 agcacttctt agatttaaaa aatgtaataa agatgaaatt ttaaatatag ttgagaattg
     3781 tttagttaaa tttgatattt taaatgatga tgatgaactt gctattatgg tttatgctga
     3841 ttctgaatat gaaataaata acagtttatg tctaataaaa gataaaggat attgcgaaaa
     3901 aatgaataaa atgttgaaaa taagttaaaa aagaaaaaat ataagtttat atgtaacata
     3961 ttttgacgat tttaatacta tatagtatga gatgaaacaa aacatctcat aaaaaaatta
     4021 aaattaaagg ggagatatgt tatatggaaa ataaaaaata tgcaaatggt ggttattcag
     4081 aagattggta tgaaagagga gaatctacag ctaagtggtt ccaaaatgat agagaagaat
     4141 atgaaagagc agcatacgat gaagatagag aaagaagagg ttcaaactgt ggaggttcag
     4201 attcagaagg aaatagacct agaaactgtg aaagatttag aagagaagct gagataagag
     4261 aaagagaagc aagagaagca ttttgtgaat cttcagagag aaagaaagaa gcattagcat
     4321 atgaatgtga agctagaaaa ttatgggaac aagcagaaaa atactgggat gaatattcaa
     4381 aatacaacta tcaaggaatc gaatatttag cagaagctgc tagattattt gatgaaggta
     4441 tggaatgtga agctagaaga aatggaaata atggaggaaa caataataat tgttgccaca
     4501 aatgccataa atgtaattgt aactgctgta gaaaataaaa aaattcattg acaaatcaat
     4561 agttaaaata tatcatatta tgtataataa agagaaaatt cagaaaaaaa taatgattat
     4621 aagaaatata agtatattta ttagacaaat agtgctcagt tagtgaggtg agtacaatat
     4681 gattaaactc ccatcctcta gctcttgata gagggtggga gtttttatta ttgatatatt
     4741 aaatgaggat aatattgtaa aattaggagg taaaaaatga aacgtattaa aacatcaagt
     4801 atgaaagaaa aggtagatga aaagatatta agagagcaag taagttctat aatagaagat
     4861 ataagaaata ataaagatat tgcacttaaa aaatacaatg agaagtttga tagaaataca
     4921 agagatgaat ttagaattac taaagaagaa ataaaggaag cttataagca tgtagatgac
     4981 gaatttataa ataacttaaa aattgctgcc aaaaatataa aagaatttgc taaagctcaa
     5041 aaatcaagtt ttgaaaattc ttttgaaaaa gaaatttatc caggtgtaat tctaggtcaa
     5101 acaaatattc caatagagtc atgtcttgca tatgttccag gaggtcagta tccattgttt
     5161 tcaacagctc ttatgcttat aatacctgca aaagtagcag gagtaaaaag aatagtggca
     5221 tgttcaccaa ctatgaaaaa tactgaaaag ataaatccta aaaccttggt agctatggat
     5281 atagcaggtg ctgatgagat atatgcaact ggaggagttc aagctatagc agcatttaca
     5341 tatggaacag aaaagattaa accggttgac ataattgtag gcccaggaaa taagtttgta
     5401 actgaagcta aaagacaatg ttatggacaa gtaggaattg actttgtcgc tggaccaagt
     5461 gaagttctta taatagctga tgaaacttca aagccagttt atattgcagc agatttatta
     5521 gcacaatgtg agcatgattt aaatgcaaga ggtatattat tgacaaatag cttagaaata
     5581 gcagaagaag ttgaaaaaaa tatagaaact atgctaaaag atttgccaac aaaagatata
     5641 gcatatagtt catggaaaaa taatggagag ataattctgg ttgatgatat ggaagaagcc
     5701 attaaaataa gtaattttta tgcaccagaa catttagaaa ttgcagtaaa taagtgtgat
     5761 gatatttttg acagattaac taactatggt tctttattta ttggaaatct ttctgctgag
     5821 gtatttggtg actatgtttc aggtaccaat catacattac caacgcttaa agcatcaaga
     5881 tatactggtg gtgtttgggt tggaactttt atcaaaactt gcacaaagca gatatttaat
     5941 gaagaagcta tagaatcatt agcacctgta gctgaaaagt tggcaaaaga agagggatta
     6001 tatgcacatg caaaggcagc tgaggttaga tttaaaaagt agtaaaaaat tctattttcg
     6061 tcagtcgaga tttagtatta gactgagata aggaaaacgc cgagaacatg aataaatact
     6121 atattttgtg ggtaatcggc gtttctttta tatctgtata tgataggata atactacctg
     6181 tgtgtttaaa tgataacttg atatttcttt tctaaataat gtttatgaaa tagttaatat
     6241 ttttagttaa tacttgaaaa aaacttttgc taactgtgca aattcatctg catttgtcaa
     6301 tatagctggg tgtcctccat gactaaaaat gtgaatactt gcttttcgaa taaaagttgc
     6361 catttgtttg tactcttctt caagattgtg acgacacatt tcgtcttcct tacttcctat
     6421 aaacaaaatt ggcatttcta atttacacaa ttctttatga aacaaaggac gtttctcctt
     6481 tgcacattgc aatagagctt ttgtatcaag attaacaaca ttttcccaat cttttccctg
     6541 acaccattca tagaattttc gtgcttgaat atcttgcttt gatttttccc ttccagtgat
     6601 tagattatct atgaagttat cattaagagt acgcccatca aagctatctg ctataattgc
     6661 ttcaactaaa tcgggacgtt ctaatccagt attaacagct gcccatgctc ctccacttgt
     6721 tccaattaag ccaactttac cacaattcaa atgttcaatc agtgctatcg tctgtaaggc
     6781 ttcatcatgc cacatgtcag gagaaaattg agaaactcta tccgactgtc cattacctaa
     6841 aaaatcaatt aagatacatc taaagttttc tacatataat ggcataagtg ctttaaacat
     6901 attagaagaa gcagtattac catgcaggaa aactatcggt ttaccttgtc catattcttc
     6961 ataaaaacaa gcattacctt gatactgaaa atatgacata tattgtcctc ctttttattt
     7021 gttaagttaa ttataacaat ataggtatga atgaaaaacc tcatgttctt tcttagaata
     7081 aaaatgtgcc acgcgtaaat cgtgacacat ctctaattat aaattttgct gtttaagtat
     7141 tctttgtatg cttttctctg tcaaaaagta tttctctgat aactgcttga tgctcattca
     7201 tttttggaag tttatacaga tttgagaatt acgagaagct aagacttgtt tggtgtttgt
     7261 gttttccccc caatgctttt tgtgctcttg ctttttggga atataaatca ctttaccatc
     7321 tatatattct tgcacttgct caatcagttc tttaggcagt aagtcaatcg aattttgata
     7381 gctcattttg cacctcctaa ataattgctc tttaggatta gcaaaggcta ctgctactca
     7441 taattaaaaa taaaattttg ttattatgta caatagcctt tgctatgcac aagttccaat
     7501 tcccataaac tcaatactcc tttctgattt aaaaaattca tctgaacatt gatttcgttt
     7561 ttaaaataga gttcaatgac aaatttacat ttctattata tatattcagg tagctaattg
     7621 caactgtctt tgtatgaggg aattttatta tagtcaatac tataatatgt atttatatca
     7681 tcaaattaat atgaacagtt gaaaggaaca tgaataataa aacttagcag gaagaataat
     7741 tataaagcag acaatagaga aatttgattc taaggttttt ttgaaaattc attaacaaat
     7801 ttaacttgta aatattatac taaacttaat aaataaagtt tataaagtaa ggagttgtct
     7861 atgaaaaata tagttggaaa atcacaaacg ataaaaaaag taaatgaaga cttaataaaa
     7921 aatattataa aaaatgaagg tcctataaca aaactagaaa tttcaagcat tactgaattg
     7981 agtttagcta cagttaataa gattgtagag cagttagctt taaaaaatga ggttaaagta
     8041 agtggactaa gtgaatctac tggaggaaga agagctcaat tatttgaaat aaatgcaaac
     8101 ttagaacaca taatagcttt atactattat agaaattatt gtataggggt agttgccaat
     8161 ttattaggtg aaataattta tcaagaaaaa tttaatataa gaatggatac gtattaaaac
     8221 gctaaccaaa atactttttt tgttatagat actttaataa aaaaaagaaa aaggaaataa
     8281 tataagagcc attggaattg gcgtcccagg agttgtaaaa aaaggtatag ttaaaaatat
     8341 accaactata aaaagttggg aaggtataaa tttagaaaat ttaataaaga gtaaatacaa
     8401 tactaaatta tttatagaaa atgatacaaa tattactacc attgggattt atcaaaaaca
     8461 gtataaagaa aaatataaaa atatggctct tatgtatcta gaaaatggta ttggttcagg
     8521 tatagttata aatgacgaat tatatttagg ttctacaaat tttgctggag aattaaatta
     8581 tctaaatata gaatttggta agtataactc taataactta aatcttgaag agtatatgta
     8641 tctggtatat ctggttgtga ttctcctaat gactataaga ttataatgga tgaaatatta
     8701 aaaattggga ttaacaaaga aaaaatatat ttagaaaatg atgcagtttt agctttttat
     8761 gcacaagctg attcacatgg tttagtaatt gtggctggga caggttcaat tatattagga
     8821 attaaagaag atggaggata atatttcaga gttcaagggt gaatggtaaa ttgtcataga
     8881 taataaataa aattttcaga gttactttat atagtttcta aagtaactct tttttaaatt
     8941 gaatttagtg ataaattatt gaatagtatt tctaaacaaa taaaaaatta atataaacaa
     9001 agacttaatt atactaaatt ataagtcaaa ctaatataaa cttatataaa aaaggaaaac
     9061 gtctaaagta ttggaattac tgtgatttag gtatggtatt aaaaaaacat aaaaaaacat
     9121 aaaaaaattg ttgaaaaaac tataaaaatt ttgtataatt aaagatttga aaagtgcggt
     9181 atacaaagcc aattcttttg tatataatat atataaattc agaaaaagga agggatatgt
     9241 atggccaggc ggttattttt agaaaggatt ggacttatat ttttaggttc agcaatatta
     9301 gcatttggag tttataattt ttattattta aacaacataa cagaaggtgg agtgttaggt
     9361 attttactac tgttaaaaaa tttattcaat atacaaccag caatagcaaa tgttgtaata
     9421 gatggtttac tacttttagt aggatataaa tttttcggta aaaaattcct tatatattca
     9481 atagtagctt ctataacttt ttcagtgtta tatgacttat ttgaagctat aggacctcta
     9541 gtaccacaat cacaaaatat gcttttatca actattttgg caggtgtaac tgttggaact
     9601 ggtgtaggta tagtagtaaa agcaggttgt gcatcaggag gagacgatgc tttagcatta
     9661 gtaatttcta agactacatc tttgaacata gggcaagttt atttagcaac agatgttatt
     9721 gtattgttgt tatcattatt ttatctatca gcttttgata tattttactc tttgattgct
     9781 gttacaatta gtgggaaggt aattgatttt atttattatc atggtaaaag tttggatatg
     9841 gatataagta atgatatagt tccagaatgt taatataaat taaataattt tatattttat
     9901 taggggtggc aagtttgcca ccttttttgt gtctaaatat ttacataaag tataacctca
     9961 tctaggtttt tataaatttc aaatataaaa ttatgttgaa atgactagga atacgattac
    10021 tagaggtgct ttttaaaaac ccaattttat atttatgaaa tactaatcat taatggatgt
    10081 ttcttaatcc tatctttata cctaaaatga taagataaat gaagtagcaa tacaaaacta
    10141 aaatttgtaa aagagattaa agtaaatgga ggagctaaga tgttggatat agttatgata
    10201 gcattgttat tcataggatt tgtttccatg aagttacttg ctgattggtg tggaaaacaa
    10261 actgaaaaaa aataatgtaa tgtatggagg gattacctat gtggtttctg gcagttgtaa
    10321 ttataatgtt aataatatat ttggtatatg cgcttattaa tcctgaaaaa ttttaaacag
    10381 tataagacta ataagtaagt tgaattgggg ggaacgattt ttatggtgca gatagtaata
    10441 gtgttagcga tttttatgat tttagtaata cctatgggca agtatttata tcatatagct
    10501 actaatcaaa aaacatttgg agatagacta tttgataaag tggacaattt tatatataaa
    10561 gtatgtagta tagataagaa aaaagaaatg aactggaaac agtatgcctt agctttatta
    10621 tttacaaatg cagttatggt atttatagga tatatcatac ttagaactca gagtatgcat
    10681 atttttaatc caagtggaat taaaagtatg gaacaaggac tatcatttaa tacaataata
    10741 agttttatga caaatacaaa tcttcaacat tattcaggtg aatcaggact ttcatacttt
    10801 agtcagatga cagtgataat atatatgatg tttacatcag cagcaacagg atattcagct
    10861 gctatggcat ttgtaagagg gttggttggt aaaaagaaaa cattgggaaa tttttatgta
    10921 gatttaatta ggataacaac aagagtactt ttaccaggag ctttaattat tgggattata
    10981 ttagttacac aaggagtacc acaaactttt gctgggacag aaacggtgac tactatagaa
    11041 ggaaaacttc aagatatagc tagaggtcct gtagcggctc ttgaatcaat aaaacattta
    11101 ggaacaaatg gtggtggatt ctttggttca aattcatcac acccatttga aaatccaaca
    11161 ataatatcaa atatagtaga aatattatct atgatgatat taccaggagc ttgtgttgtt
    11221 gcatttggtt atatgataaa gaataaaaaa caaggttggg ttgtgtttgg tgcaatgtca
    11281 atcatatttt taataggttt agtagtttgt tttaaagcag aaagtgcagg aaatcctatt
    11341 ttatcacaat tagggcttaa tcaaagtatg ggaagtatgg aaggtaagga agttagattt
    11401 ggtatagcac aatcatcatt attcacagca gtgactactt catttacaac aggtacagtt
    11461 aataatatgc atgatacact aactccttta ggaggattag taccactttt aaatatgatg
    11521 ttaaatgtag tctttggtgg taaaggcgta ggtcttatga atatgttgat gtacgctata
    11581 atagcagtat tcctatgtgg attaatggta ggaagaacac cagagttttt aactaagaaa
    11641 atcgaaggaa aagaaatgaa gcttattgct ttacttatta tattacatcc acttttaata
    11701 ctaatgttct ctgggctttc ggtagcagta ccagcagggc ttgaaggaat ttcaaatcct
    11761 ggattccatg gattatcaca agtattgtat gaattttcat cttcagcagc taataatggt
    11821 tcaggatttg aaggtctagg agataacact atgttttgga atatgacaac aggtatagtt
    11881 atgttctttg gaagatatgt gtctattata gtattactag caatatcaag tttgttagca
    11941 tctaaaaaag ctgtaaatga aagtatagga acacttagaa cggataattt tacatttaca
    12001 atagttttag tacttgttgt tttaattgtt ggtgctttaa catttttccc agccttagcg
    12061 cttggaccta tttcggagca tctagtgtta tggcactaaa aaattgggag ggagtaaagt
    12121 tttatggata ctaaaaaaac aaagtttatt acaaaagata ttttcaaaac ttccattata
    12181 gaagctttta aaaaattaaa tcctaaatat atgatgaaaa atccagttat gtttgttgta
    12241 gaaattggat ttatagtaac attgatactc acaatagtac ctagtttgtt tggtgatgaa
    12301 ggcaataatt taagagttta taatgggatt gtaacaataa tcttatttat aacagttctt
    12361 tttgcaaatt ttgctgaggc tgtagctgaa ggaagaggta aagctcaagc agatacactt
    12421 aagaaaacta gaaaagatac agtagcaagg gttatagata cgttaggaaa tgaaaaaatg
    12481 ataagtgcaa gtgagctaaa acttggtgat atcgttttag taaatgcagg agaagttatc
    12541 ccaaatgatg gagaagttat agagggtata gcttctgtgg atgagtcagc aataactgga
    12601 gaatctgcac ctgtcatgag agaatctggt ggagattttg catctgttac aggtggtact
    12661 acagtggtca gtgactggct aaaaataaga ataacaacaa aaccaggaga atcattctta
    12721 gacaaaatga tagctcttgt agaaggtgct tcaagacaaa aaacgccaaa tgagattgct
    12781 ttaaatactt tgttagtagg gttaacactt atatttatgg ttgttttaat atctctttat
    12841 ccaatggcaa attatgcagg tgtaaagatt ccaatttcta ctatgatagc tcttttggta
    12901 tgtttaatac caactactat aggtggattg ttgtcagcta ttggtattgc aggtatggat
    12961 agggtaacta gatttaatgt aattgcaatg tcaggaaaag cagtagaagc ttgtggagac
    13021 gttgatacta tgattttgga taagacaggt acgattactt atgggaatag acttgcatca
    13081 gaatttataa cagttggaaa cgcagattca aaagacttaa taaattattc tgttatgtgt
    13141 tcattaaaag atgatacgcc agaaggaaaa tctatagttg atttaggcat gaaattaggc
    13201 tcaacaggta agacaaaaga ggcagaggaa gctgagtttg tagaattttc tgcacagact
    13261 agaatgagtg gtattaacct agctgatggt agagctataa gaaaaggtgc ttatgatagc
    13321 ataataaaaa gagtaaaaga atctggtggt atagtaccag aagatttaga agaaaatgta
    13381 aatcgagtag ctaaactagg aggaacacct ttagtagtgt gtgtagataa tgggatatat
    13441 ggaattatct acttaaagga tacagtaaaa tcagggcttg tagaaagatt tgctagactt
    13501 cgtgagatag gaataaaaac aataatgtgt acaggagaca atcctttaac agcagctaca
    13561 attgcaagag aagctggagt agatggattt attgcagagt gtaagccaga agataaaata
    13621 gatgctataa agaaagaaca atctgaaggt aaaatagttg caatgacagg tgatggaaca
    13681 aatgatgctc ctgcacttgc tcaggcagat gtaggtcttg caatgaatag tggtacaaca
    13741 tctgcaaaag aagcagcaaa catggtagat ttagattcag acccaactaa gatattggaa
    13801 gttgttgaaa taggaaaaca gcttctaata actcgtggag ctttgacgac atttagtata
    13861 gcaaatgacg ttgcaaaata ttttgcgatt ataccagcta tattcactct tgcaatacct
    13921 caaatggaga tattaaatgt aatgaaactt tctacatcat ttagtgcgat attatcagct
    13981 ttaatattca atgctataat tatcccatgt ttaatacctc ttgcaatgaa gggagtaaaa
    14041 tatagaccta tgaaatcaca agctatgtta atgagaaata tgcttatatt tggtcttggt
    14101 ggagttatag taccattttt aggaataaaa ttaattgatt tattgattac acctttagta
    14161 atggttctta atttaggtta ataaatggag gatataaaaa tgaaaacatt aaagccagca
    14221 ttacttgtaa gtattgtatt gttagttgtt tgtggacttg tgtatccact tgttttgaca
    14281 ggtgtaagtc aagttgcatt caaagataaa gctaatggaa gtatgataga agtaaatggt
    14341 gtaaaggtag gttcagaact tattggtcaa agctttacag atgcaagatt ctttaaagga
    14401 agagtttcat cagtaaacta taatacttat acaaaagaag atttagtacc agataaagat
    14461 ggaaatacat cttatggagg tgtatcatca gggtctttta attatggagc tacaaatcca
    14521 gaacttcgtg atagagtgca aaaagatata gaacagtttc taaaggataa tccaactgtc
    14581 aaaaaagaag atataccaac agatttgctt acagcatcag gttcagggct tgaccctaat
    14641 ataagtccgg actctgcaaa aattcagata cctgctgtag caaaagcgtc aggaatatca
    14701 gaaagtaaat tacaaaaaat tgtagatgat aatacttcca aaaaagtttt tggagtttta
    14761 ggggaagata gagttaatgt actaaaggtt aatgtagagg tcgcaagaat attaggtctt
    14821 atatagcatt ttgtagaatt ttagtaacaa aatatcatgc aagatttaat ttaaaggtta
    14881 gattttaaag agtgaaaatc gataatgatt ttcacttttt tatttgaata ttactaatat
    14941 ttggaataaa gcgattgtaa gtataaaatc caaaaaaagg ttgcattatc aaaatgataa
    15001 tactataata tatttatcgt tatcaatttg ataatagatt ttgacatata ggaggaaata
    15061 gtatgttata taataatgca ttagagttga taggaaacac acctgtagta aggctgaata
    15121 atttaggttg tactaacggg tgttctaaca tatatgtaaa attagaaaaa gtaaatccag
    15181 caggaagtat aaaagataga gccgtttatg aaatgctaga aggtttagag aaaagaggag
    15241 aattaaaaaa gggagatgta ctagtagaag caactagtgg aaatactggt attgcattat
    15301 ctatgatagg aaaattaaag ggatatgaag tcgtaattgt aatgcctgaa acaatgagta
    15361 ttgagagaag agatttaatg aaggcttatg gagcaaattt ggtacttaca gatggtaaag
    15421 ctggaatggc aggttccata gaaaaagcaa atgaactttt aaaagaaaat ccaaactata
    15481 aaagcttaaa acaatttgaa aataaagata atccaaatgc acattataaa acaacagcag
    15541 ttgaaattat gaatgatgtt aaagatttag atatatttgt atgtggagtt ggaacaggtg
    15601 gtactttaat aggaactgca agatatttaa aagaacaaaa tccaaacata aaagtagttg
    15661 cacttgaacc aaaaaaatct ccagctatat cagaaaacaa gactggacct cataaaatac
    15721 aaggtatagg tgctaacttt gttcctgaaa attatgattc tagtgtagtt gatgaaataa
    15781 tattagtaga tgatgaagat gcatttgata cagttaaatt acttgcatca aaagagggaa
    15841 ttttagtagg gatatcttct ggagcaaata tatttggtgc tatagagatg gcaaaaagat
    15901 atccagataa aaagatagtt acagtagctc cagatggagt tgataagtat atgtcgatgg
    15961 gaatatttta ataattttat ttgagggagt gaatactttg tttaagaaaa taaataaaga
    16021 tattgaatat ataatgaaaa atgatccagc agctaggtct aagatagaag tttttttact
    16081 ttatccatca gttcatgcaa tgataatgca taggatggct catgctcttt ataagaagaa
    16141 gaagcttttt acagcaagat taatatccca gatatccaga tttatgactg gtatagaaat
    16201 acatcctggt gctaaaatgg gagaaggtat attgatagac catggaatgg gtgttgtaat
    16261 tggagagaca gctgaagtag gtaatagggt gacaatatat caaggagcta ctttaggagc
    16321 cacaggaaaa gatactggaa aaagacatcc aactgttggt gatgatgtat taattggtgc
    16381 tggaacaaaa atactaggtc ctttaaatat aggttcaaat tcaaaaattg gtgcaaattc
    16441 ggttgtcgtt aaggatgtac ctaatggtgc aacagttgtt ggtataccag caaaaatagt
    16501 aaaaataaga aatctagaac ctgtaaaaaa gaataaaaaa gaagtagact atgagtatga
    16561 tgaattagat aatgtatatt atatataggt tttgtataac cataaaataa actttataaa
    16621 aattattttt aaaaggtatt tttttcaaat ttgagggcat aataaaaaag aactgaatgt
    16681 tacagttttg gttcatcaat ttttgataaa caagatttta aatttgtagc gtctaggagg
    16741 tttttattat gaaagcaaat gttaatcaag atacttgtat aggttgtgga ttatgtccat
    16801 caatatgtcc agaagtattt gatatgaatg atgatgggaa atcacatgtt atagttgatg
    16861 aagtaccaag tgatgctgaa gaatcagcag cagaagctag agaaagctgt ccagttgatg
    16921 caatagatgt gcattaataa aataaatgcc tataaaaatt gaattttcaa tttttatagg
    16981 catttgtctt ttattcagtc agtatttcaa cacctgtttc tgtaactaaa accatacatt
    17041 cccattgagc agataattta ccatcttttg tataaatagt ccaattattt tcttcatcaa
    17101 cgaaaatttc gtgagttcct tcatttacca taggttctat tgtgaatatc atcccaggaa
    17161 ctaataacat ctcagtccct tttttagata cataacttac aaaagggtct tcgtgaaagc
    17221 ttaatccaac tccatgacca ccaatttctc taacgacaga gtatccattt ttctttgcat
    17281 aagagttgac agcatcacct atatctccta agaatcccca tggtttggca acctcaagac
    17341 ctttttggac gcattccttt gtaacttgaa ctagtttttt tgcattttcg ttaacattac
    17401 ctaccataaa cattcttgaa gcgtctgaat agtatccatt aaaaatagta gaaacatcta
    17461 catttataat atctccatct tttaagataa tgtccttatt tggaatacca tgacaaactt
    17521 cattatttat tgatgtacat acgcttttag gaaatccatt atagcctagt ggagcaggaa
    17581 tagctccatt ttgtagtgta aattcatgaa ctaacttatt tatatcttct gtagatattc
    17641 caacttcgat tttatctgaa atataatcca aaatttttgt gtttattaca gcactttttt
    17701 ttatttcctc aatttgactt tcatttttta taatgtcatg agttggtaca atatgaccta
    17761 atttagcaaa tgaatctatt ttattatcca tatccatatg acatttttta tattttttag
    17821 tacttccaca ccaacagcta tcatttctat ttaacttcat aacttttcat ccttctttta
    17881 ttatttattt aaattatggg ttacataaac tattttaaaa cataaccatt ataaatatta
    17941 taacataaat actaaaaaat cctttataga tagtgattta ttcaaaatat aaattatcat
    18001 gtgattattg ttgatgaaaa gagtacagtt aaatgtatta ttggatagta gtacacttaa
    18061 tagtatagtt caagtttttc aagaaaatca tatgtcagag gacaattttc ctttaaagta
    18121 ttttttgaat cttcatcaag ataatagtgt acaaatgatt ctgcaaaaaa ttcttctata
    18181 aacttgctta tatatgcatg tgcaatttgt ttagggtcat aaagtttgta tctttcctga
    18241 gcaaatactt tcataaactc ttcagaattt gattttttat ttaaaacgtt tttatctata
    18301 gcatgtgcag tttcatgtaa ttctaaattt ttagatgaat gtacatttgt atagctagga
    18361 ttactataac ctattttagc aactatagaa ttatttctat atataccagg tacatctttc
    18421 caagtcttgc ctgatcttac ccagcatgga ggtaattgat attttaaatc actaaattct
    18481 ggttcatcag ttagatttga atttattaat tttattttta tatccttttc tttaagtgct
    18541 tgtaaatatt taatatttat ttttgcaagt ctgcttttca tattttcaac ttcatcttta
    18601 tcataattca agtcatcagg aagtataagc atttgttcaa ttatatctat gttactgtct
    18661 tttatatctt cagactcttc atcaaattta tattgaagat tatcattttc atagatagtt
    18721 atatttgtat tattttccat agaataagag tgaatatcct ctaagtttaa aatattcatt
    18781 tgttgtaaaa tatgttttgt ttcgccactc catactacta aagaaagtga attaaaatta
    18841 attagtggat tttcttttgc ttttatatca ttgcttatat cgacatcaaa tacatattca
    18901 attgagtcct ctatgttaag gccatttgta ttctttttaa tatacaaata cgcttgtttt
    18961 aagtctatat aaatctcatc taaagattcg tcataaaatt ctgataaatt attagtaaag
    19021 agttcttcac ttaaaagttc atttttatca gttatacttt cgattaaaat attaatttct
    19081 tttatcatat cgtaaatact tatttttcta tttgcttctt cactagatac attgcatggc
    19141 gcaagagcaa tgcaataaaa atctttttct tttttataaa aggctttaag tgattttgat
    19201 aaaagtgtaa agtctacacc tatctgtaaa tttaccttaa gtgaatcatt ttgtgataat
    19261 tcagtattat ttcttttagc tgtatttaaa ttattttcca taatcattgc ccccttttta
    19321 ttagggtatc ataatttttt tatatgtgtc aatttatttt tttgaaaatt ataatttttt
    19381 tgaaaatttt attttttata atttgttaat tttttattat agaaataaag atgttattat
    19441 tatgtcaaaa tttagcatta gattcatgta gagtatattt tatatacata tattataatt
    19501 aactttttga agattgcata ttcaaaatac acaccatttt ttttaattct ataatataat
    19561 atttatatat ttaatgttag gatgtgattt aagatgtatt ttacatatat tataagatgt
    19621 aaagatgatt ctctatacac tggatatacc agtaatatag ttagaagaat gaatgaacat
    19681 aagttaggag ttaattcaaa atacacaaga gcaaaagggt ttaaaaaact agaagtttat
    19741 tttgtgacta atacaaaaag taatgctatg aagttagaat actatataaa aaaattaact
    19801 agaaataaaa aattgtctat aataaaaaat ccaaatatac ttataaattc aattaataat
    19861 aaaactgatt atattattgg gaaagaaata gaataattaa cataaacatt catagtaaca
    19921 ttataatgag tacatatagt gtaaaaactt gacaataaat actattgtaa tataatgaag
    19981 ttatgaatat agtaaattaa aatagctagg ggagccaaga gctgagaggt ataccgaccc
    20041 ttacacctga tctggataat accagcgtag gaaagcttag taaaaattca agtatgtatt
    20101 atttgtgata tatgctattt ctaagctata cctattgtag gtatagcttt tttaattaag
    20161 ttatttaaat taaactttag gaggcaataa tgagtaatta taaaatacca acacttacaa
    20221 tagccggttc tgactcatct ggaggagctg ggattcaggc agatttgaaa acctttagtg
    20281 caattggtac ttatggcatg agtgtaataa cagctataac agcacaaaat actcaaggag
    20341 tttttgctgt agaggactta aataagaata taataaaaaa acaaatagag gcagtctttg
    20401 aagatatacc tccaagagct gtaaaaatag gaatggtttc aagtcctgag attatacttg
    20461 aaatagttaa gaatctaaag aaatacaatc caaaatattt ggtggtagac ccagtaatga
    20521 tatctaaaag tggatattat ctattaaagc cagaggcaaa agaaaactta attaagtatt
    20581 tgataccact tgcatacata attacaccaa atataccaga agcagaagaa atcacaggaa
    20641 taaaaataca taatgtagat gatatgaaga gagtaggaaa agaaatactg caacttgggc
    20701 ctaaatttgt acttatgaaa ggaggtcatt tagatggaga agcggtagat attttaattg
    20761 gtaaaaatat atttaaagtt tataaaagtg aaagaattga taaaaaaaat acacatggaa
    20821 caggatgcac attatcttct gcaataacat cttatctagc attaggatat gaaataacag
    20881 aagcagtaaa ttttagcaaa atctatataa cagaagcaat aaaaagaagc tttgatatag
    20941 gtcatggagt aggaccagta catcattttt ataaatttga ataataaaaa aatattggag
    21001 gtagaatatg tataatttaa ttaaagacgt aaaaaaatta aatccattgg taattcatta
    21061 tactaataat gtaactataa atgattgtgc taatgcaact ttagctgtag gagcaagtcc
    21121 tcttatgagt ttttcatatg aagaagtaga agaaatggta agtgtagcaa actctgtagt
    21181 aataaatata ggaactatga actcaaatat gttggatttg ttcctcttag ctggaaaagc
    21241 agcaaataag tacaataaac ccgttgtttt ggacccagtt ggagtatttg caagcaaagc
    21301 gagagcagaa cttacaagta gacttttaaa tgaagtcaaa tttagtgttg ttaaagggaa
    21361 tgtgtcagaa ataaagttta taggtggttt taatgtaagg ggtaaaggcg tagattcatt
    21421 tgatgaagaa gattctactg atataataag aaaagttgct aaaaagcttg aatgtatagt
    21481 tgtagcaact gggaaaatag atataataac taatggtaaa agtacataca aaataaacaa
    21541 tggaactgat aaactgaaag gaattacagg cactggatgt atgactgcta gcttaattgc
    21601 aagttttatg gctgttactg agaatatatt agaagcagca acaatgggtg ttcttacaat
    21661 gtctttaagt ggcgaattgg ctaatttaaa taatccgcca ataggtacat ttaaagaaaa
    21721 tcttatgaat gctatttatc aaatggacat agatacttta agtaaaaatt ctaatattga
    21781 atttttaaat taataaagag taagggagac ttaaaatgat agataaagaa tctttaaaaa
    21841 aatgcttaag attgtattta ataactgact ctgaaatgct tgaaggtaga gacttttata
    21901 aatgtattga agatgcaata tcttctggaa taacaactgt acagcttaga gaaaaaaata
    21961 cttgtggaag agaattttta agaaaagcta tgaaacttag agaaataact aaaagatatg
    22021 gagttaagtt tataataaat gatagagtgg atatagcact tatttgtgat gcagatgggg
    22081 tacatgtagg acagagtgat attgatgtga gagaggttag aaaacttata ggaaacgata
    22141 aaattctagg agtatcagca agaactttag aagaagctat ttgtgcaaaa aatgatggtg
    22201 cagactattt aggcataggg tctatattct caacatctac taaattagat gcaaaatcag
    22261 cctcatttga aactgttaaa gaaattaaag aaaaagtaga catgccattt gtactaattg
    22321 gaggtataaa tttagacaat atagataaac ttaaatgttt agatagtgac gggtatgcta
    22381 taatatcggc tatactaaaa gctgaagata tatctaaaga agtagaaaag tggacattaa
    22441 aaatataaaa ttggctctag tagtgaaaaa aatggaaaaa tttgatataa tctaagagta
    22501 aaatttaaga aattataaat gataaacttt tctacatata ctttttaatc aagatatatg
    22561 tagatttggt taaaaaggag ttataaatga ttaggatatt gttagtatgt gttggaggca
    22621 tgtcttccac tctattggta aataaaatgg agaaggacgc aaaaaagaga aatatagatt
    22681 gtaaaatatg ggcagttgga gaaggagata taaaatcaga gcttgataac ttcgatattt
    22741 tacttcttgg accacaactt agatttatgt ttgatgatgt aaagtctata gttggagata
    22801 gagctccagt gtctataata gatatggtaa actatggtac ttgtaatggt catgctgtgt
    22861 taaattcggt tttagaaata ttaaaataaa taataaaagt ggattctata taatagagtc
    22921 cacttttatt atttgttatt aatggattta aaacttagct taccatttct ttttttaatc
    22981 tccacatagc aagtgaaaaa cttgcacagc acaatattaa aaatagtagg ataacaggta
    23041 gtatatcttc aataagaaca gtctcaccaa atattaagct acgtaaagcg ttaataacat
    23101 gggtaaatgg attgagcatc actaccactt ttaatcctcc agataaattt tctataggga
    23161 aaagagctga acttaaaaag aaaataggaa gtacaattgc agtcataatt gtttcataaa
    23221 ctatctcatt tggcaacaat aaacttatag aataagctag acttgataaa aagaaggcgg
    23281 tcataaatac aagcactgtc ataagtagga ttccaccaat gccagattct atcgttactg
    23341 aaaagaatat acttactata cacaaaatag ctacttctat gaaggaaact aagatagctt
    23401 ctaataattg accaaggacg attgaatacc tactgatagg tgcaattaag actctataga
    23461 aacttccact attcttcatt ataaagttga tgataccacc actactacaa gaagaaaaca
    23521 caaccagtat aattatacct ggtagtataa aagcagtata attattgata tttatattat
    23581 tcatagtttg gtttgcaata gaactataaa gcacaagcca aagaaaaggt tgtaatatag
    23641 taactataaa tgagattgga tttttaaaac gccacttcat actacgccat aagatattta
    23701 acattcccat ccctccttta ggcttttttt agaacttgta agtgctaaaa atacatcttc
    23761 gagacttggt tccacaattt ctattgcatc aaattctata tcatgttcta aaagccattt
    23821 atttattaaa gtaaaagcag ttctactatc gtttacactt ataaaaattg aattttctcg
    23881 tacattcata aactttacta aaccagtacc cttaattgca tcattatatt tttttatatc
    23941 ttcagtatta tggaaagtta tacgtattat attttgtcta atgtaactac gtaaactgga
    24001 tggtgttcct tgtgctaaat cttttccatt cttcataata caaatgttat cacttaattg
    24061 ttcagcttct tcaagataat gagtagttag aaatatagtt gtgccatact catatctaat
    24121 ttttaataac atatcccaca tagactttct tgaatctaca tccataccga cagttggttc
    24181 atctagaaat agaattttag gagatgatac catattcata gctatgtcca atctacgttt
    24241 tacaccacct gaatatgatg aagtaggata ttttaagtaa ctagataagt caaacttatc
    24301 aattaaagag tctattcttt gttttgcaat ctgtggtttt actttataca tcttgctttg
    24361 aaatatcata ttttccataa gagataagtg ttcatcaata gagatttgtt gtgctacaca
    24421 agcaatttga gtacgtatcc aagaaggatt atctactaaa tcttttccaa acattgttac
    24481 atttccagat gtaggcttgt aaaaagtagt caaaatattg ataagagaag atttaccagc
    24541 accattaggg ccgagcaaag aaaaaatttc tccagaattt acattaaagc ttagaccatt
    24601 taaagctttc acaccatttt tatattcttt tactaaattg tctatttcaa ctgctaacat
    24661 atattattcc tcctctttaa gtggaaatat tatctctgta atatagttat ctggttttcc
    24721 ttttaccaac atacctggtc ctttaataaa tacttctctc caaggagttt gtatttttat
    24781 attattttca tgaatatatt taaatatcat ttcatacgtt tttggcaaat tactataaga
    24841 acctatatga gtaaaaaaca aagcttttgt tttaggaaat tcttttgtag ttatacccat
    24901 gcgatttgga gtctcagcag ttggaacaca taattccatt tctgcaacac cagttttttg
    24961 gtcaacatca tagtagcaaa agaatggtgc accgttagac tttcctttta tagctttaaa
    25021 tacatttgga aaatattttg ttgcctcagt cataggtcca ttataacgca tggtagctac
    25081 tgtgactgat tcaatatttt tgattttata actcatttgt ttacctccta atgtgtattc
    25141 atatttttat tagattttta agcatcaata catataagat tttgattagt agaaagcata
    25201 taatcttatc tcgtttgctt gtgttcaagt ctaacataac ataaaatata tatcctctca
    25261 tttgatgctc cattttgtaa agttattatt taagctttat tttagttttt tagtaaattt
    25321 aaattaaaat aaaataatgt ataaaaaaag tgtttacaaa ctaagtgtat taatcgtata
    25381 atacacttaa taggatacta gataaggtct gaaataaaga catatttttt taaagtaagt
    25441 gtactatcgt attaatacac tagaatagga ggaaccatga actttgaatt agataataca
    25501 actcccattt atttgcaaat agtaaagtat ataaagagac aaatagtaat aggagagttg
    25561 aaaccagggg atacaatacc atcaagaaga gaaatggcac ttaatctaaa agtaaattta
    25621 aatacggttc aaagagcata taaggagatg ggggatatga atataataaa tacttttaaa
    25681 aatcatcaaa gcagtgtaac aattgatgaa aatatattaa aaaatttaaa attagagctt
    25741 ataaatgaat cattatctgt ttttttaaaa gatatgaaag caataaatgt taccaaagaa
    25801 gaagttttaa agataataga agataagtat tagtttttta acatatagaa aacttagttt
    25861 agaaagttta tttataagca tattggatgt ggaggatagt aaatatgata gaaataaaaa
    25921 atgtcagcaa aacatataaa aggatgcaag ggcttaaaat aagaaagata gaggctttaa
    25981 aaaatgtaag ttttaatata gaaaagggaa aaataactgc attacttggt ataaatggcg
    26041 ttggtaaatc aactatgcta aaagcaatag cagggcttat aaacactgat agtggagaga
    26101 ttcgtataga tggagaaaaa ataaatgaaa agatatacaa taaattggct tttgttccag
    26161 atgtacaatc tcacttcagt aatacaacaa taaaagaaac atttgaattt atggaaattt
    26221 tttattcaaa gtggaataaa aaaaaatcaa aagaaatgat gaacatattt aaattggatg
    26281 aagatgaaat aattgataac ttgtcaaaag gaaacattgc aagagtaaaa ttaatacttg
    26341 gattttgcca agacccagag tacatacttc ttgacgaacc atttacagga atcgatttgt
    26401 ttaagaggga agaatttata ggagtaatag cgcaatatat ggaggaaaat caagctatta
    26461 tcataactac tcatgagata gtagaaatag aaagtttagt tgatgaggtt ataattcttg
    26521 atgaagggca gattatagct tcatttaatg cagaagaatt aagagaaaga gaaggtaaat
    26581 ctatattaga taagatgagg gaggtatata agaatgaata aacttctata tctaataaat
    26641 gtagatttga gaagaagcaa taagttttat atagcttata tatcattttt ttcattaatt
    26701 acacttggtt taaatttatt cgaaattaat aaatttaaaa attctaaatt tattataaac
    26761 ttagcaatag gagattttgg aggtatattt tatggaatag gtatacttag taatcttggt
    26821 tttatccaaa gtatattgtt ctttgggatg ttgggacttt ttatatacta tatttttatg
    26881 tggaaaagag agtttttttc gaataatcaa agtatttata cactaatgat gttacctcaa
    26941 aataagttta agatttgtat atctaaatca atagctttgt taacaatgat atatggtttt
    27001 ttgataagtc agatagcaac attatttata tgtaaatata tatttaattt tatttttaga
    27061 aatgtgccaa taatcaatat gaactttgcc aaagatttat attatttaaa tttgaagggt
    27121 ataccaattg attttatctc ttttatggct atttatgtat ttttactact tatagctatt
    27181 tctattgtaa attgttgtat attatttatc ttttcctata taaataaatt ttatatactt
    27241 ttaatcactt tgttgattat agtaggctta tatttttcaa caatatatat aagtataatc
    27301 actttattta gaaaatatta tatggctata ggatttcaat ttaataattt agtagcagga
    27361 ttattaagtt ttttaattat aatgtttatt ctaaatggaa tatcttattt gttaattaaa
    27421 aagaaaatat ctctttagga ggggtggtat gaaattaaaa tctaaaatat ctatacttat
    27481 tatatttttg actttagcaa tatattttag tataggttta ttaaataatg aagataactt
    27541 aaagataaaa actataaaag gagatcctaa tgaattagac tatataaaca taaattttgg
    27601 atatgctttt aatggatatg taaattcata ttatacaata aatggaagta acaaaaccaa
    27661 aaagactgaa ttaataaaac tacaaaaaga tgctaagaac acaaataatt taattgatgt
    27721 atctagtggt tcagatttaa ttgctcatgt tggatttgaa atgaatgatg actcaaaaga
    27781 tataaacttt gtaatagatg aaaaaaaaga agatttagaa aaaggtgtat ttccatcaga
    27841 aggagatgat aaagcagttg aatatgaaaa actagatatg aatgatttta caaggacaag
    27901 atataaagta gataaaagtt ctgataatta ctatgcaaat atactatcat caaaaagata
    27961 taaaaaatac ttatatgtat ctgtacttta tgctacaagt gattcatcaa aaaacaacaa
    28021 agaatattca agtactatag atataataaa aataaatcca gaaaataaaa aaatccagac
    28081 tataaaaagt ataaatttaa gtaattattt ttctaaaaaa taccaaaata tagataatat
    28141 aagtgtaagt tatgctagca aatatgagga taagatgtat attttagtaa atgtattaaa
    28201 tgcaaataca ggtgataaaa gtaattctaa atttcactta cttgagtata atcttgaaga
    28261 aaataaggaa agcataatgg aattaaaaac tgatagcaaa atggctttag caaaagtaaa
    28321 gttagaagat aataaattat atatggtaat gtcaaatgac tatgataata gttcaattac
    28381 agagttaaat ttacttacct ataatatatt agataaaagc ttagatgaag ataagtatat
    28441 tttagataat aaagaaaaaa gaggaattag aagtattgaa attgattcaa ataaaatcat
    28501 tgtatataca tcaaggttaa attctacatt atcttgggat aataaattta tgttatatat
    28561 aataaataaa aattctaagg aaattgtata tgaaggagaa ttacaattta acagttcaaa
    28621 tagtatgtta gaatttgaaa
//
LOCUS       DAFNNB010000032        28454 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0032, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000032 DAFNNB010000000
VERSION     DAFNNB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 28454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28454
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0032"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            294..515
                     /locus_tag="L3M60_001259"
     CDS             294..515
                     /locus_tag="L3M60_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBN6225943.1"
                     /translation="MGYLKEDRQDYCCEFARPYVNRQIYDDTYNPKTALKRGTLFPEL
                     DLIESNNYNDWLYANPKNRVRAKKGGNMY"
     gene            515..778
                     /locus_tag="L3M60_001260"
     CDS             515..778
                     /locus_tag="L3M60_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBN6225944.1"
                     /translation="MSLNRRELLERISEYQFACIELNLYLDNNPRDKKALDSYNRYCD
                     KFTQAVCDYESRYGALTNFGYESSEHPWSWISEPWPWDKSFYK"
     gene            842..1414
                     /locus_tag="L3M60_001261"
     CDS             842..1414
                     /locus_tag="L3M60_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBN6225945.1"
                     /translation="MWIYQKTLEHPVNIRQADPRMAKYIMTQLGGPNGELAAATRYLQ
                     QRYTMPTGKSRALLTDIGTEEMAHVEIISSVLYQLIGSCTPEELKAAGLGSNYANFGH
                     GLQPVDSNGVNFTTSYINVFGDSVTDLHEDMAAEQKALATYYQLINLTDDPDLKDILR
                     FLGEREVVHYQRFGEALMDVYEFTECKHQF"
     gene            2206..2859
                     /locus_tag="L3M60_001262"
     CDS             2206..2859
                     /locus_tag="L3M60_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HBN6225946.1"
                     /translation="MNFIYSNAEIEYLKHKDKILSDIIDKIGHIEREIDTDLFSSVIH
                     HIIGQQISTKAQATIWKRMKEHLKEINASSILSAGTTGLQSFGMTYRKAEYLTDFATK
                     IQNGEFDLQSITYMSDKEAIDKLTSLKGIGVWTAEMILLFCLQRPNIFSFDDLAIQRG
                     LRMVYHHRKITRKMFEKYQKRFSPYCSVASLYLWAVAGGVIDGMRDYAPKGKVRKNG"
     gene            3021..3554
                     /locus_tag="L3M60_001263"
     CDS             3021..3554
                     /locus_tag="L3M60_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBN6225947.1"
                     /translation="MQYINYYHSPIGNILLASDNIGLTGLWFENQKYYAYNLDQEHQE
                     KELPIFEQTKKWLDIYFSGEEPNFTPSLHMVGTPFQTAVWKILQQIPYGKTITYGEIA
                     YKVAQQKGISKMSAQAIGGAVGHNGISIIVPCHRVVGTNRSLTGYAGGIDRKIKLLSL
                     EKVSMQHYFIPKKGSAL"
     gene            complement(4108..4893)
                     /locus_tag="L3M60_001264"
     CDS             complement(4108..4893)
                     /locus_tag="L3M60_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HBN6225948.1"
                     /translation="MDEKILELLQEMQGNISNMQGSISGIEGTISDMQGNIGDMQGSI
                     SGIEGTISGMQGTISDMQGNIGDMQGSISGIEGTISDMQGNIGDMQGSISGIEGTISS
                     VQGTIIDMQGTISDMQGNIGDMQGSISGIEGTISGMQGNIGDMQGSISGIEVTISGMQ
                     GNIGDMQGEIKGVNSRLDNMEPNIKDLKTGQVETSSKLNTIQSDVTEIKSDVKINRNT
                     LDTIIKHTSSLVEDTSDIKNDLNNIEIVTSKNWNDIAKLKSVK"
     gene            5357..5770
                     /locus_tag="L3M60_001265"
     CDS             5357..5770
                     /locus_tag="L3M60_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003507530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBN6225949.1"
                     /translation="MKMPKENVDMIMFAPCGMNCIVCYKHCYHKKPCAGCLNSNMGKP
                     EHCRKCKIKDCVCQKGLFYCFECFDFPCKLIKNLEKSYNKRYQVSLLENSKFVQKYGL
                     EKFMEKQRERYTCSKCGGIISIQDRECSECREKMK"
     gene            5844..6380
                     /locus_tag="L3M60_001266"
     CDS             5844..6380
                     /locus_tag="L3M60_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBN6225950.1"
                     /translation="MIGLKCGMVKLLKHQIIWDKSAKDVIILLKSILDKTAIDIQHIG
                     SSSIPSVSAKPIIDIVVGVASLEEAKLYIKKLEQRKIIFRGQDIPEQLLFVMGDLKKN
                     IRTHHIHVVEWNSFAWNNYINFRDYLNTFTEKAKLYDSNKQKLALQFEDNRKIYTQRK
                     QELISTLLEEARLWRMNT"
     gene            6541..8115
                     /locus_tag="L3M60_001267"
     CDS             6541..8115
                     /locus_tag="L3M60_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HBN6225951.1"
                     /translation="MKKDTSHIIKRAEHFQVILIGEGLMVGAVAGLIVLLYRILLGQA
                     GTWLSQILKFVEGSTIKIIAWFAVLAVLAWIVSKLVDWEPMISGSGIPQLEGEMTGKI
                     KQVWWRVLPTKFIGGFLSLMGGLALGREGPSIQLGAMAGKGISRALDRGKTEEKFLLT
                     CGASAGLSAAFHAPLAGVMFSLEEVHKNFSVSVLISVMTASLTADYISANFLGMESVF
                     QFDIGNVLPQDYYGLIVILGVILGVMGAFYNWFTLYVQYLYKKIPKIGTFGKLLIPFI
                     MAGILGLVMPDVLGSGHNLVEELTSHEYIFGMVVLIFVIRFIFSAVSFGSGAPGGIFF
                     PLLVLGAFIGGIFGMVGVKVFGMNPDYVNNFVLLAMAGYFTAIVRAPLTGIILIFEMT
                     GSVSQMLSLSVISIVAYIVATLMKSKPIYESLLERLLEKNGEPVHKERGEKILDQFSV
                     MFGSPVANKAIQDIQWPENCLLVAIQRGGEEIIPRGKTILRPSDVIVTMTDERDSGAV
                     YDKMENLCKEHTSHIF"
     gene            complement(8804..9409)
                     /locus_tag="L3M60_001268"
     CDS             complement(8804..9409)
                     /locus_tag="L3M60_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea carboxylase-associated family protein"
                     /protein_id="HBN6225952.1"
                     /translation="MINWNLWRTLNMEYIINPCSGIKIKVTKGQKIEIVDIEGGQVVD
                     FFAEMQDDSAEFISPGVTIDCNESLRLQVGNLMYSNKYKPMFKILYDDVREHDLLHPC
                     CKLEMYDFFYNNGENHPNCFDNINQALGTSHQIIHPINLFMYTKLNTDGSIEVCEPLS
                     KANDKIVLEALENMTLGIAACSVSESSCNSRKCTSIKIVVD"
     gene            9780..10349
                     /locus_tag="L3M60_001269"
     CDS             9780..10349
                     /locus_tag="L3M60_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HBN6225953.1"
                     /translation="MKKKLEDMTLEELWNLFPIFLTTHKECWKNWFKEEQYRLTEILP
                     MQKIVRISHIGSTSVSTIYAKPIVDILIEIIPDYNMKDIKDLIESSGYICMSESSSRL
                     SLNKGYTVDGFEERVFHLHIRYVGDNDELFFRDYLIDHPSVAKEYEKLKLDLWKKYEH
                     NRDAYTDSKLDFVREFTEKAKMLYSNRYN"
     gene            complement(10686..11960)
                     /locus_tag="L3M60_001270"
     CDS             complement(10686..11960)
                     /locus_tag="L3M60_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBN6225954.1"
                     /translation="MTFLLYKLTSYFIDFRNHWIFKIILIYGYSIISQMIIWISDPVN
                     ILFTLFGYFVLLIICSKSRLIPIVSVIMILYPIIVGINFLFINNPLYRELIISKEYIL
                     ILSIVQIVSALIKLLFWFLIHIFLKNRLVNIKQYLSNKIWIFIDMVCISSFLSILIAI
                     ILPPTNLIPMGSNKNIILGNLSYGTYLIILSAILANIGVILLLQPLIENVKMKIEKQA
                     NHLKEKYYNLLENQQIQIRKMRHDMNNHFQMIESYLEIEDIAGAKKYFNQLKIGIGTL
                     SGKQFCKNQALNSILNLRHTSLEENGVDVHFNIDIDNHLGIEPIDLCTIFSNSLDNAI
                     EASLKIPDLSNRKVVLKARCEKEYFSYLLTNNKVNPINRKQELFISDKKDSNSHGYGI
                     ENIREIVNKYNGKLDISYTENEFSLFLYIRLF"
     gene            complement(12254..12430)
                     /locus_tag="L3M60_001271"
     CDS             complement(12254..12430)
                     /locus_tag="L3M60_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225955.1"
                     /translation="MFFTFIGVISAILLLCTIIYGLWIKLNNRNNDSNSHFTFSILSI
                     AFSLLIVVAFLVRN"
     gene            complement(12534..13142)
                     /locus_tag="L3M60_001272"
     CDS             complement(12534..13142)
                     /locus_tag="L3M60_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6225956.1"
                     /translation="MEKFLRGKIMKADSTKEQLINATIQLLSSHRDVSSITAREIVAK
                     ANVNLAMINYYFKSKEELINTSIKKFLKRHMDEYKATHDTKNLAPKQQLREILIAFFD
                     FIGEHSQFIKISVPYILIQEEIVYPFEILPLIKAHYENHNNKKTEAEYRTIAYQITSF
                     IQLIFLRSNAFLKYSGIDILNVEQRNGLIDFQLDLFLVDITK"
     gene            complement(13238..14062)
                     /locus_tag="L3M60_001273"
     CDS             complement(13238..14062)
                     /locus_tag="L3M60_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6225957.1"
                     /translation="MFKIGEVSKLTQISIRMLRYYDELGILKPAEIDKYTGHRLYSVE
                     QISTLQRIVLLRDSKFSIAEIANIIHDWNDEFVIKELNRKKNEIQKEIKQEQQRINKI
                     DKFIEAINCDKDEIHYNVVFKKIPSYKIISIREIIPDYQSEGILWEKLSKFIKEEHIE
                     VSRQPNNNIAFYHDEEVKDNGVDIEVGMVVKKIGKNKSGFIYRETEEIDMMACTMVYG
                     PYENIAGAYESFCYWLDKNSDYQISGINRQIGHRGEHNEINCENYLTEIQIPLIQV"
     gene            complement(14228..15121)
                     /locus_tag="L3M60_001274"
     CDS             complement(14228..15121)
                     /locus_tag="L3M60_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6225958.1"
                     /translation="MQLYNTPFNTNKNLIYFFTLTLVLNWIFSLTPALLGITNTILGE
                     IIFYLAYTSPAIVGLFFISTIYPQSAKQDFIYRYLSFKEMGIKWPLLTILFFALTFIT
                     SLTIGNYFNVKTSDIDWRQIVILRPSQIAYMLLVSFILSILSQEAGWRGYAIDKLLVR
                     FGFIGSSIILGLVCGIWYLGSYFTPNQIPYNLAQYSLFDAFLFIPNIVLLNFIINFVY
                     INTSRSILSAGLVHMMYYFFSVQLLLHYPIKLCVIAQYVQIIFGLIFLVYMISSNKFK
                     QKVNSEIEQIKSDDFEFELDL"
     gene            complement(15375..15812)
                     /locus_tag="L3M60_001275"
     CDS             complement(15375..15812)
                     /locus_tag="L3M60_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HBN6225959.1"
                     /translation="MKIHVEQGINCDDIEITIKCSTLDNNIEKIISLLSTPLLDIKGK
                     KSGEIYKLNVCDILYFETVGNKTFAYYEDDVFEIDLKIYKLSKLLANTSFSRISKSMI
                     LNMDYIKSIKVISHNRMQATLRNNEKVIINRCYIKNIKEKLNI"
     gene            complement(16069..16218)
                     /locus_tag="L3M60_001276"
     CDS             complement(16069..16218)
                     /locus_tag="L3M60_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvrJ family protein"
                     /protein_id="HBN6225960.1"
                     /translation="MDDSLQLLITNVGFPIALSMYLLVRIEGKLNTLSNSIDTLSKNI
                     LLFKK"
     gene            complement(16223..16462)
                     /locus_tag="L3M60_001277"
     CDS             complement(16223..16462)
                     /locus_tag="L3M60_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HBN6225961.1"
                     /translation="MEIKKKLVMSFKNVKDKQVSFSIENPKDDLTEENIKSVMDLIVS
                     KNIFSVGGFDLASVVEAKVVETNTTPYDLVIRIGG"
     gene            complement(16787..17008)
                     /locus_tag="L3M60_001278"
     CDS             complement(16787..17008)
                     /locus_tag="L3M60_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HBN6225962.1"
                     /translation="MEVVSTKNLSSIKLKLDAGFDDKGKAVIKSKSFANVKAEALNED
                     VYAVAEAIASLQENPIVDILKLDSTSLSK"
     gene            17587..17844
                     /locus_tag="L3M60_001279"
     CDS             17587..17844
                     /locus_tag="L3M60_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6225963.1"
                     /translation="MKIKVEDIPVQFHAMVEIIGIDKFIEVTKLYGGTNTYIPTYNGL
                     FRYARNREIVKQFNGVNYNELAIRYNMCVSNIKRILNENSV"
     gene            18292..19530
                     /locus_tag="L3M60_001280"
     CDS             18292..19530
                     /locus_tag="L3M60_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBN6225964.1"
                     /translation="MNNGFYKLNSAVLNFDIKSDVNKIRFNHYIMRKENIQRLQGTLP
                     RGQFYMTVRRAATDLQLSISTISRLVREFLDLGIIRLVSKGVKGNRSVYSYVSIEFNG
                     SLFDDSKHMELDCVCNDEFVDNSDIELDCNSKDNSFNGDINVETEYCSNEYLFNNDVE
                     HNYDNFYNKDTIKNKSIFNISTIKKYINESFDTNKSENSNLQQESINFNFNDTCNAID
                     KRTNNSIKSNQNKKAMKLSDINVLSDKDITNKKNYFNHTHGIQKGTKSVTKKKESLNK
                     FNKKNIQQSIINMLNKKSGKNFKIDSPISIKLINERLKEGYVLEDFYKVIEVKVAKWK
                     NTIMEMYIRPETLFSYKFESYVNENISMKSQSNIHPCKEGNSNDSSNYIHSNYTTYDD
                     SVRERKKYENVRYWNPICEI"
     gene            19999..21300
                     /locus_tag="L3M60_001281"
                     /pseudo
     CDS             19999..21300
                     /locus_tag="L3M60_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437698.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            21376..21651
                     /locus_tag="L3M60_001282"
     CDS             21376..21651
                     /locus_tag="L3M60_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6225965.1"
                     /translation="MLELTKIIELLEEHKIEVSIPKELHKYSLLEYLELCQQSGGTYF
                     YIKTLEGALKEARNELIIKLFNGKNYMELARMFEMSVINVRHIVRKK"
     gene            22709..22939
                     /locus_tag="L3M60_001283"
     CDS             22709..22939
                     /locus_tag="L3M60_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6225966.1"
                     /translation="MSNKKKKYANILANECVACGSCIKACPRSAISVPCGISAKINRD
                     LCVGCGICEKICPASVIEIITILKEEEGKCHE"
     gene            22932..23513
                     /locus_tag="L3M60_001284"
     CDS             22932..23513
                     /locus_tag="L3M60_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6225967.1"
                     /translation="MNNKKHWYDYLWIFSSVYLILGFVNIIFAWIGLICFFVPLAISI
                     VKGNKAYCNKYCGRGQLFNLLGNKLKLSRNRDIPKFIRSKWFRYGFLTFFMVMFANML
                     FSTYLVFEGSRNFKEVVTLLWTIKMPWQWAYHGTLVSPWVVQFAFGFYSVMLTSTVLG
                     LITMMLFKPRSWCVYCPMGTMTQMICKAKSKSV"
     gene            complement(24087..24764)
                     /locus_tag="L3M60_001285"
     CDS             complement(24087..24764)
                     /locus_tag="L3M60_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBN6225968.1"
                     /translation="MISQNEIDILTHSLPFWDKLTDLQKELLISSANTSHYKKGNPVH
                     CGDSDCIGILIIKSGTIRTYILSDEGREVTLFRLDDGDVCILSASCILKTITFDVYVD
                     AETDCDIIQISSSVFAKLSTENIHAELFSYKLATERFSDVMWAMQQILFMSFDKRLAS
                     FLIDEIAKNGSSTINMTHEQIAKYMGSAREVVSRMLKYFAREGIVSLSRGGIKVLDKD
                     KLRSLTL"
     gene            25295..25816
                     /locus_tag="L3M60_001286"
     CDS             25295..25816
                     /locus_tag="L3M60_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6225969.1"
                     /translation="MATIDLIVLGIVKQQPKSAYDIQKEVEYRNISRWVKISTPSIYK
                     KVIQLEQKGYIESKTVKNGKMPEKAVYSLTELGETHFLDLMFDTSSKPINILLDFNAV
                     IVNLNSLTPEKQKLCLNNIEENVQELKKTIEENISIKTEVPETAKAVLEQQLVLAQSL
                     ETWINSLKDNMGN"
     gene            26199..26996
                     /locus_tag="L3M60_001287"
     CDS             26199..26996
                     /locus_tag="L3M60_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6225970.1"
                     /translation="MEEKYILSDRGKVYYWIKESNSVFAKNLIFLPGLTANHHLFDFQ
                     IQYFSKNHNVLVWDAPAHGKSRPYLDFSYSNLVEELKVILDKQNMKRVVLIGQSAGGF
                     VAQSFIVKYPNMVEGIMMIGTCPYGIDYYSKLDIFWLKQTEWMLKLFPDRLLRNIISK
                     MCGATSEGRRSMLKMLEGYEKKELCHLMYLGFAGLIPEIQDLNIHCPVCLVVGDKDKT
                     GKVRKYNEQWHNKTGYPLHIIKNASHNVNVDKPNELNQIMDIFIKQL"
     gene            27376..28266
                     /locus_tag="L3M60_001288"
     CDS             27376..28266
                     /locus_tag="L3M60_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6225971.1"
                     /translation="MIESIPAKQILQKVKFDNTRWFGIDYNMNLYRGCSHGCIYCDSR
                     STIYNIENFDKVRYKENVIEILSKELRSKRKKGVVGIGAMSDTYNPFEKQLCITKKAL
                     DLISENHFGVSIDTKSSLVVRDIPILQKIKKNNSAIVKLTITTANDELSKKIEPYVNP
                     SSVRFEAVKELNDSGIFCGILLTPMLPFLTDNKDEIRAIVEKAHKANAKFIYCMYGVT
                     MRSGQREFFYEHLRDISPKLVLKYQKTYGLNYVCTVQNKDGCEKLLREECARYGILTD
                     MKEIIKAYKRSESYTQIKFF"
BASE COUNT        10621 a         3278 c         4607 g         9948 t
ORIGIN      
        1 attgtatttt tatagtgttt ttaataaaaa ataatcacgt tttatctaaa aatgaataat
       61 atatagtaaa tagtaattaa gttataaaaa attgtataaa ctctatatta ctcatttaaa
      121 atagatattt atgaaagatt aattagaaaa ttttagaggt aaaataagaa caaaaaaata
      181 cttatttaat cttattaaag acctattaaa actactgatt ataatataga acaagtatac
      241 aatttttaag aataatgcta ttttaaaatt ctaaaaatta aggaggataa ttaatgggat
      301 atttaaaaga ggatagacaa gattattgtt gtgagtttgc tagaccatat gtcaatcgtc
      361 aaatttatga tgacacatac aatccaaaaa cagcattaaa aagaggtact ttatttcctg
      421 aattagattt aatagagtca aataactata atgattggct atatgcaaat cctaaaaata
      481 gagttagagc taaaaaaggg ggtaacatgt actaatgtca ttaaatagaa gagaactttt
      541 agagagaatt tcagaatatc aatttgcttg tatagaatta aatttatatt tagataacaa
      601 cccaagggat aagaaagctc ttgattcata taacagatac tgcgataaat ttacacaagc
      661 tgtgtgtgat tatgaatcta gatatggagc attaactaat tttggatatg aatcaagtga
      721 acatccttgg tcatggatat ctgaaccttg gccatgggat aaatcatttt ataaataaaa
      781 ttattaagat ttaaaattag aatttattat taaataatat attaaggagg agtaagataa
      841 tatgtggatt tatcaaaaaa cactggaaca tccagttaac ataagacaag cagaccctag
      901 aatggcaaaa tatatcatga ctcagttggg agggcctaat ggtgagctgg cagctgcaac
      961 tagatatctt caacaaagat atactatgcc aactggaaaa tctcgcgcac ttttaactga
     1021 tataggtaca gaggaaatgg ctcatgttga gataatttct tcagtgttat atcaattaat
     1081 aggcagttgt actccagaag aacttaaggc tgctggactt ggcagtaatt atgctaattt
     1141 tggacatggt cttcagccag tagattctaa tggagtaaat tttactacaa gttatattaa
     1201 tgtctttggc gattcggtaa ctgatttaca tgaggatatg gctgctgaac aaaaagcatt
     1261 ggctacgtac tatcaattaa taaatttaac tgatgacccc gatttaaaag atatattgag
     1321 atttttgggt gaaagagaag tcgttcatta tcaaagattt ggtgaagcac taatggatgt
     1381 ttacgagttt acagagtgta agcatcaatt ctaatgaact attaaaagtt gacagagagt
     1441 aagccttagt tttaatgaac tattaaaagt gaaccagaga gtaagcatta attctaatga
     1501 actattaaaa ataagaggta aaggttttag ttgatttcct ttatctctta ttttttactg
     1561 attaaacaga ttactttgct atttaaacat attattttat caaagaaaag ctcaatcaaa
     1621 aattcaaagc atcaccatag ttcaaggtat taccgttgtt ggcaagtttt ctacaatatg
     1681 ttttctttaa aacatttact aaaataactt atttgatgaa aaacaatttt tatagcagta
     1741 ttacttatta gctcatttgt acattttaga aattgagttt cttttaataa gcgaaactga
     1801 gtaaaatact tatggggtgt aatatttaga cttagtttaa agcatatgga gtactatgat
     1861 ttacttatat taacactgtt tgataagcat ttcaaagtta agttttcaca atagtgtttc
     1921 taaatataac gtaaaatttt ttgcatacag atattaactg tattttgtat tttattagtg
     1981 gcaaaacaat attttttatc ataataagcc aaatggaaca aatagaaact gatgtttcat
     2041 acatataaag ttcatttttt ctttttcaca gcctattctt acttcctaca tttgagagta
     2101 ttataatatt atttgatgtt atatcgatat ttttttgacc tatttagtga taaaattata
     2161 atatgagctt atctaaaagt taatatagta ggagggctat gatatatgaa ttttatatat
     2221 agtaatgctg aaatagagta tttaaaacat aaggataaaa tcttgagtga tataatagat
     2281 aaaatcggtc atatagagcg tgaaatagat acagacttat tttcgtcagt aatacatcac
     2341 attattggac aacaaatatc aacaaaggca caggctacta tatggaaaag aatgaaagaa
     2401 catttaaaag agataaatgc aagctccatc ttatctgctg gtactacagg attgcagtct
     2461 tttggtatga catatcgtaa agccgaatat ttaacagatt ttgctactaa aatccaaaat
     2521 ggtgaatttg atttacaaag tatcacttac atgtctgata aagaagcaat tgataaattg
     2581 acctcattaa aaggtattgg ggtttggaca gcagaaatga ttttattgtt ctgcttacag
     2641 cgtccaaata ttttcagttt tgatgattta gcaattcaaa gaggattacg aatggtttat
     2701 catcacagaa aaattactag aaagatgttt gaaaagtacc aaaaaagatt tagcccttat
     2761 tgtagtgttg caagtttgta tctttgggca gttgcaggtg gggtgataga tggaatgaga
     2821 gattacgctc ccaaaggtaa agtgagaaaa aatggttgaa tatatgcagg taaagataaa
     2881 actagtatga tatgaggata gatattataa cgaaagtgca atcaggagga aattatacta
     2941 gtatgttttt acataatggc aggtagaaaa gaagtgtcta atgactcgaa tgaaattttc
     3001 agaatataag gaggcattgt atgcaatata ttaactatta ccatagtcca attggaaata
     3061 ttcttttagc atcagataat attggattaa ctggtctttg gtttgagaat caaaagtatt
     3121 atgcttataa tcttgaccaa gaacatcagg aaaaagaatt gcctattttc gagcagacaa
     3181 agaaatggct agatatttat ttttcaggag aagaacccaa ttttacacca tcactccata
     3241 tggtaggtac tccttttcag actgcagtgt ggaagatttt acaacaaata ccatatggta
     3301 aaacaatcac ttatggagaa atcgcatata aagttgctca acaaaaagga atatctaaaa
     3361 tgtcagcaca ggcaattggt ggtgcagtag ggcataatgg aatctctatt attgttccat
     3421 gtcatcgtgt tgtaggaaca aacaggagtt tgacaggata tgctggtgga atcgatagaa
     3481 aaataaaatt gttatcttta gaaaaagtgt ctatgcaaca ttattttatc ccaaagaaag
     3541 ggagtgcatt ataaagtcat caaatttaga aattcttaat cattcctttg atatacaagt
     3601 atctgatttt gaaagcaata acttgcaatt taaaaaaaga ttttttagac tatatgttat
     3661 acaatattat ccatagtaaa cattatacaa ttttagagat aggttttgga gtttttattt
     3721 tatcattgta caaatgttca aaatatgttt ttggaaatga taagagtgtt aaatccaaag
     3781 gaaaaaatga aaacaagatt ttatcctttt ataagtgata agggtatgtg ttttaatcaa
     3841 agatggactt ttactttaat gctaaaaatt atatagccat attataatat ataagggaga
     3901 ggcacacctt tttagtgttc aatctccctt gttttttatt attatatcaa gactcattca
     3961 gtcgtgttaa ttttatgata aaatgagtat tttttaataa attatttata agtaaagtgt
     4021 gttatcggat aatcagtatt tttgtataaa atttggtata aatttaaaga agtatgtgaa
     4081 attttcaggt gtttttacta tagtatatta ttttactgat tttaatttag caatatcatt
     4141 ccaattctta gatgtaacta tctctatatt gtttaaatca tttttaatat ctgatgtatc
     4201 ttctactaat gaagaggtat gtttaattat tgtatctaat gtatttctgt taatttttac
     4261 atctgactta atttctgtaa catcactctg aattgtatta agtttactac tagtttcaac
     4321 ttgtccagtt tttaagtctt taatattggg ttccatattg tctaatctac tattgactcc
     4381 tttaatttct ccttgcatat caccaatatt accttgcatg ccactaatag tgacttctat
     4441 accactgata ctgccttgca tatcgccaat attaccttgc atgccgctaa tagtgccttc
     4501 tataccactg atactgcctt gcatatcgcc aatattacct tgcatatcgc tgatagtacc
     4561 ttgcatatcg atgatagtac cttgcacgct gctaatagtg ccctctatac cactgatact
     4621 gccttgcata tcgccaatat taccttgcat atcgctaata gtgccctcta taccactgat
     4681 actgccttgc atatcgccaa tattaccttg catatcgctg atagtacctt gcatgccgct
     4741 aatagtgcct tctataccac tgatactgcc ttgcatatcg ccaatattac cttgcatatc
     4801 gctgatagtg ccttctatac cactgatact gccttgcata ttactaatat taccttgcat
     4861 ttcctgcaat aactctaata tcttttcatc catcactatc acactcctat tttattatta
     4921 taacataatt taggagttgt attccacaaa cgctatatca tatttaattt ttaatcatat
     4981 cattatctaa tactcaaaac atggttaaat atgtttatat tgaaaacttt tttattaatg
     5041 aaccttatat tgtctaagtg tctaatatac tatgataagt gcttttgagg tatgtgtggt
     5101 tacatctgta tttaaagttg ctggtactat catatacttt taaataccac ttaagctatt
     5161 tatattgatt acttgtgttt gaatattttg attagtagat ttacttttat ctttttttat
     5221 tcttagtttt aaactttcat atttccttga aaatttattg acaattaaag tgcatgaatt
     5281 ttttagttac tttttatgtt acaatatgaa tatataacaa ttcgacaaat tggatttttg
     5341 aaaggagtaa aatgttatga aaatgccaaa agagaatgtt gatatgatta tgtttgctcc
     5401 ttgtggaatg aattgtattg tttgttataa gcattgttat cacaaaaagc catgtgctgg
     5461 ttgtttgaac agcaatatgg gaaagccaga gcactgccgt aagtgtaaaa taaaggattg
     5521 tgtttgtcaa aaaggattgt tttattgttt tgaatgtttt gattttcctt gtaaactcat
     5581 aaaaaatctt gaaaaaagtt ataacaagag atatcaggta agccttttgg aaaatagtaa
     5641 atttgttcag aagtatggtt tagagaagtt tatggaaaag cagagggaga gatacacttg
     5701 ttctaaatgt ggaggaatta tatctattca agatagggaa tgtagcgagt gtcgagaaaa
     5761 aatgaaatag ctaaatacct gtttatcaaa atagaaaaaa ttgtagtatg tatataaagg
     5821 ttgattatgt tgaggtggtt atagtgatag gtttaaaatg tggtatggtt aaattattga
     5881 aacatcagat aatatgggat aagagtgcaa aagatgtaat tatattgttg aaaagtatat
     5941 tggataagac tgctattgat attcagcata ttggaagtag ctctattccc agtgttagtg
     6001 caaagcctat tattgatatt gttgtaggtg ttgcttcttt ggaagaagct aaattataca
     6061 tcaaaaagtt agagcaacgt aaaattattt ttcgaggtca ggatatacca gaacaattac
     6121 tttttgttat gggagattta aaaaaaaata ttcgtactca ccatattcat gttgttgaat
     6181 ggaatagttt tgcatggaat aattatatca attttcgaga ttatctaaac acttttacag
     6241 aaaaagcaaa actctatgat agtaataaac aaaaattagc attacaattt gaggataatc
     6301 gaaaaatcta tacacagaga aaacaagaac taataagtac tttgctggaa gaagcacgac
     6361 tgtggagaat gaatacttaa aaagaataaa ttccaattta ctgagaaatt taacgataat
     6421 tttcttatac ttagtaaata aaaatctatc tttggtgatt aaaaaatagc tgtaaacact
     6481 cgtgactttg gtcatgggaa atttagattg tttagaatta ttggatgaag gaggaaacgt
     6541 atgaaaaagg atacatctca tattataaaa cgggcagagc actttcaggt aattttgatt
     6601 ggagaaggac ttatggttgg tgctgtggct ggtttgattg tgcttttata caggatactt
     6661 ttagggcagg ctggtacttg gcttagccaa attttaaaat ttgtagaagg ttctacaata
     6721 aaaataatag cttggtttgc agtgcttgca gtacttgcat ggattgtatc aaagttggtg
     6781 gactgggaac caatgatttc tggaagtggt atacctcagc ttgagggtga aatgacaggg
     6841 aaaataaaac aagtatggtg gcgagtgttg ccaacaaaat ttattggggg attcttgtca
     6901 cttatgggtg gtcttgcttt gggaagggaa ggtccttcca ttcagcttgg tgctatggca
     6961 ggtaaaggta tttctagagc acttgaccga ggaaagaccg aggaaaaatt cctgttaacc
     7021 tgtggagcaa gtgctggatt atcagcagcc tttcatgcac cactggcagg tgttatgttt
     7081 tcacttgaag aagtccataa aaacttctca gtatctgttt taatttctgt aatgacagca
     7141 tctttgacag cagactatat ttcagccaat ttcctaggaa tggagtctgt attccaattt
     7201 gatattggaa atgtattgcc acaggattat tatggattaa tagtgatttt aggtgttatt
     7261 cttggagtta tgggagcatt ttataattgg tttactctat atgtgcagta tttatataag
     7321 aagattccta aaattggtac gtttggaaaa ctactgattc catttataat ggctggtatt
     7381 cttgggcttg ttatgcctga tgtgctgggt agtggacaca atttagttga agaacttaca
     7441 agtcatgaat atatttttgg aatggttgtt ttgatttttg tcatacgttt tatattttca
     7501 gctgtaagtt ttgggtcagg tgcaccaggg ggaatctttt ttccactact tgttttaggt
     7561 gcatttatag gtggaatatt tggtatggta ggtgttaaag tttttggaat gaatccagat
     7621 tatgtaaata actttgtgct tttggctatg gcaggatact ttacagctat tgtgcgtgct
     7681 cctctgactg gtatcatatt aatttttgaa atgacaggtt ctgttagcca aatgctttca
     7741 ctatcagtaa tttcaattgt ggcatatatt gtggctacac ttatgaagtc taaacctatt
     7801 tatgaaagcc ttcttgagag gttacttgaa aaaaatggag aacctgtaca taaagagagg
     7861 ggcgaaaaga tactagacca gttttctgtt atgtttggtt ctcctgttgc gaacaaggca
     7921 attcaggata tacagtggcc tgaaaattgt cttttggtag caattcaaag aggaggagaa
     7981 gaaattattc ctagaggtaa aacaattctt agaccaagtg atgtaattgt gactatgaca
     8041 gatgagagag atagtggtgc tgtgtatgat aagatggaaa atttatgtaa agaacataca
     8101 tcacatattt tttagggata atgatgaatg tttaataatg atagaatatt taatgaagat
     8161 ttgataatta aaaattggta atttgaaaga gaattaatga gttaatataa taaaatgaaa
     8221 atgtgatatt ttagatgtaa aagagtttga agaaaagagc tggatgtatt tgttaatgag
     8281 attatgtgga ttaatggtat atttatattg tgctaaaagt tagtctattg tatgagtaat
     8341 gggaacaaag ggcttgaaaa aaataggtag aagatttttt agatattctt attgggatat
     8401 tttaattaat atattttgat tgcgatattt tgggaagaat agaatttatt tataaatgaa
     8461 aataattaaa aggggttaag gttagatgaa tatcgataag gaagtattat tagataatta
     8521 acttaacatc taacagatag agtttttatg aaaacagtgt gtattgaaat aaaaaggtac
     8581 acgctgtttt ttcgttctgt tacgcaatag aatgctattt tttagagtac gagtataaaa
     8641 cccatattcc cttgtaattg tgtctgaaaa acatcataaa atcaagggat ttttacacat
     8701 aaatgtgtaa cataccataa ttaattgagg acgcaagtgc ttattctttt gcgcccttga
     8761 tggtattttc tgatttttat atttatttga taaatgaaat ttgtcaatca accacaatct
     8821 taatagaagt acattttctg ctattgcaag aactttctga aacactgcac gcagcaatcc
     8881 ctaaagtcat gttctccaat gcttcaagaa caattttatc attggctttt gataaaggtt
     8941 cacatacttc aatacttcca tctgtattga gttttgtata cataaataaa ttgatggggt
     9001 gaatgatttg atgggaagtt cccagagctt gattaatatt atcaaaacag tttggatgat
     9061 tttctccgtt gttataaaag aaatcgtaca tctcaagttt gcaacatgga tgcaataagt
     9121 catgttccct tacatcatca tataaaattt taaacatagg tttatactta ttagaataca
     9181 ttaaatttcc aacttgtaat cttaatgact cattacaatc aattgtaacc cctggagata
     9241 taaattcagc tgaatcatct tgcatttctg caaaaaaatc aacgacctgt cctccttcaa
     9301 tatccacaat ttcaattttt tgccctttag ttactttaat ttttatacca ctacaaggat
     9361 tgattatata ttccatattt aatgtcctcc ataaattcca atttatcatt ctgttctatg
     9421 ttctcatttt aacacatact ctgtctcttt tctatttgtt tcttgacgtt tataacatag
     9481 tctatcattt tttaattttc ataaaggtgt tgtagtcttt tttcaagatt acagcatttt
     9541 tttgcttaaa tttggcattt tcgtaatatt actgtattta gaagtaagaa aaaatattct
     9601 atatttaaat agatataaaa gtgtaagaaa ggtaaaaatc tgttataata cattaaaaac
     9661 taaatttaga aaaatttata taattagaaa aatgatttga taaaaaataa agttaaatac
     9721 taactaagta aatttgaaga agaaaaaata tgaaatacaa atttaaaagg aggtaactaa
     9781 tgaaaaagaa gttggaagat atgactttag aggaattatg gaacttgttt cctatatttt
     9841 tgactactca caaagaatgt tggaaaaatt ggtttaaaga agaacaatat agactaactg
     9901 aaatattgcc aatgcaaaaa attgttagaa taagtcatat tggaagtact tctgtgagta
     9961 cgatttatgc caagcctatt gttgacatac tgattgagat aattcctgat tataatatga
    10021 aagatattaa agatttgatt gagtccagtg gatatatttg tatgtctgag agcagtagta
    10081 ggctttcact taataaaggg tatacagtag atggatttga agaacgagtt tttcatttgc
    10141 atataaggta tgtaggtgat aatgatgagt tgttttttag agattactta attgaccacc
    10201 caagtgttgc aaaggagtat gaaaaactta agttggatct ttggaagaag tatgagcata
    10261 atcgtgatgc ttatacagat agtaaactag attttgttag ggagtttact gaaaaggcaa
    10321 agatgttgta ttctaataga tataattaga aatagatata attagagatt tatcttatat
    10381 atcgcattgt attttttaag aaagtaatta atagaaaaag ggattgtttc aaaatggaga
    10441 gataaatccc tttttcattg ttggaatggt aaaaatatat ttgatttcat ataaaaaaga
    10501 gtgcctcatc aactaaaaag tttattttga gacacataca tttttactaa ggtaaataaa
    10561 aatatcacat ttgattaaat tttaagttaa tgaatatatg tgcaaatatt taattataag
    10621 ttagtacaat tttaatcagt ataaaacaat ctaatttata ttatataaga taatctaatt
    10681 ttagactaaa ataatctaat ataaagaaat aaagaaaatt cattctcagt ataagagata
    10741 tccaattttc cattgtactt attaacaatt tctctaatat tctcaatacc atatccgtga
    10801 gaattactat cttttttatc cgaaataaat agttcctgtt ttctattgat aggatttacc
    10861 ttgttgtttg tcaataaata gctaaaatac tccttctcac aacgtgcctt taataccact
    10921 tttctatttg acaaatcagg aattttcaaa gatgcttcta tagcgttatc taaagaattt
    10981 gaaaaaatag tacataaatc tataggttct atacccaagt gattgtctat atctatatta
    11041 aagtgcacat caacaccatt ttcttccaaa cttgtatgac gaagatttaa aattgagtta
    11101 agagcttggt ttttgcaaaa ttgtttgcca ctaagagtac ctatacctat ttttaactga
    11161 ttaaaatact ttttagctcc agcaatatct tcaatttcta aatacgattc aatcatttga
    11221 aagtgattat tcatatcatg tcgcattttg cgtatttgaa tttgttgatt ttctagtaag
    11281 ttatagtatt tttcttttag gtgatttgct tgtttttcta tcttcatctt tacattttca
    11341 atcaatggct gtaaaagaag tataacacct atatttgcca gaattgctga caagatgatt
    11401 agataagtgc cataagataa gttgcctaag ataatattct tattactacc catagggatt
    11461 aaattagtag gtggcagaat aatagcaatt aatattgata aaaatgaaga tatacatacc
    11521 atatcaataa aaatccatat tttatttgat aagtattgct ttatattaac caatctattc
    11581 tttaggaaaa tatgtattaa aaaccaaaat agtaatttaa taagagcaga aactatctgg
    11641 actattgaaa gtattaaaat atactctttt gagattataa gttccctata caaagggttg
    11701 tttataaaaa gaaaattaat cccaactata atagggtaaa gtatcatgat aactgatact
    11761 atgggaatta atctgctttt gctacatatt ataagtagta caaaatatcc aaatagagta
    11821 aataaaatat ttacagggtc agaaatccat attatcattt gactaataat agagtaacca
    11881 taaatcaata tgattttaaa aatccaatga tttctaaaat ctataaaata ggaagtcaac
    11941 ttataaagta aaaaggtcat agagtaagga gtgttgtatt gtaaaaaata gtgtaaataa
    12001 tgtagagtaa atggaagaaa aggagttata gagcagtaag gttattaaga ctctgcaata
    12061 caacaaaata ctataataaa ataaataagc ctctgtaata taataaaaat tagtaataaa
    12121 taagattcta caatataata aaaatactat aataaaataa taagcctctg taatataata
    12181 aaaatactgt aataaataag actttgcaat ataataaaaa tctataataa taaaacccaa
    12241 cataataaag acattaattt cttactaaaa aagccactac aatcaacagt gaaaaagcga
    12301 ttgaaagtat tgaaaaagta aagtgagaat tagagtcatt attacgatta ttaagtttta
    12361 tccataaacc atatataatt gtacacaata ataaaattgc tgatattact cctataaatg
    12421 taaaaaacat caaaattcct cccccttatc taattcaagt atttttagtc tatgagaata
    12481 ttttcaagca ttaccataaa ataaaaaaat gtacataaaa aatcaatata gtattatttt
    12541 gtaatatcta ccaagaataa atctaattga aaatctatta aaccatttct ctgctcaaca
    12601 ttaagaatat caataccact atacttaaga aaagcattag aacgaagaaa aatcaactga
    12661 ataaatgaag ttatctgata cgcaatagtt ctgtattcag cttcagtttt tttgttatta
    12721 tgattttcat agtgagcctt aattaaaggt agtatctcaa atggataaac aatttcttct
    12781 tgtattaata tatagggcac agaaatcttt ataaattgac tatgctcacc tataaaatca
    12841 aaaaatgcaa ttagtatttc tcttaattgc tgtttaggtg ctaagttttt agtatcatgt
    12901 gtagccttat attcatccat gtgacgcttt aaaaattttt taatagaagt attaattaac
    12961 tcctctttag atttaaaata ataattaatc atagctaaat tgacatttgc tttagccaca
    13021 atttctctag ctgtaatact tgaaacatct ctatgtgaag atagtaactg gattgtagca
    13081 ttaattaatt gttcctttgt tgaatctgct ttcataattt ttccccttaa aaatttctcc
    13141 ataaaaaaat taaacatgtt taacacacgt ttagatacta tcatgaaaat tttgggatgt
    13201 caatgggtta ttagtactta tatacttaag tactaattta gacttgtata agagggattt
    13261 gaatttctgt aagataattt tcacaattta tttcattatg ttcaccccta tgaccgattt
    13321 gtctatttat acccgatatt tgataatcac tatttttatc taaccaataa cagaacgatt
    13381 catatgcacc agctatattt tcatatggac catacaccat ggtacatgcc atcatatcaa
    13441 tttcttctgt ctcacgatat ataaatccag atttgttttt tccaattttc tttacaacca
    13501 ttccaacttc aatatctaca ccattatctt tgacttcttc atcatggtaa aaagctatat
    13561 tattgttggg ttgacgtgat acttctatat gttcctcttt aataaattta gataactttt
    13621 cccagagtat accttcagat tggtagtctg ggattatctc tctaattgat atgattttat
    13681 aacttggaat tttcttaaat acaacattgt aatgaatttc atctttatca cagtttatag
    13741 cttctataaa tttatcaatt ttatttattc tctgttgttc ctgctttatt tccttttgta
    13801 tttcattctt ttttctattc aattccttaa ttacaaattc atcattccaa tcatgtataa
    13861 tatttgctat ttcagcaata ctaaattttg aatctctcaa taatacaatt ctttgtagtg
    13921 ttgaaatctg ttcaactgag tataatctat gacctgtata tttatctatt tctgccggtt
    13981 ttaaaatacc aagttcatca taataacgca acatacgaat agaaatttgt gttagttttg
    14041 aaacctctcc tattttaaac atgaaaatat gacctccttt tcacttataa tcagtatata
    14101 ttttgttttt atatacttca agagtttgtg gaggtacaat tattttccag atgtcgcaat
    14161 aaattatatt taagtagtaa tcgtatcaaa ttatagatta tcttaaattt tataattcat
    14221 attaaaatta caagtctaac tcaaattcaa aatcatccga ttttatctgt tcaatttcag
    14281 aatttacctt ttgtttaaat ttattagaag aaatcatata tacaagaaat attagaccaa
    14341 atattatttg tacatattga gctattacac acaatttaat agggtagtgt agaagtaatt
    14401 gaacgctgaa aaaatagtac atcatatgaa ccaacccagc cgataaaatg cttctactcg
    14461 tatttatata tacaaaatta attataaagt ttaaaagtac aatgtttggt ataaataaaa
    14521 aagcatcaaa tagggagtat tgtgccaaat tatatggtat ttgattaggt gtaaaatacg
    14581 accctagata ccagattcca caaacaagcc ctaaaataat tgaagagcct ataaatccaa
    14641 atctaactaa taatttgtca atggcgtatc ctctccatcc agcttcttga ctaagtatac
    14701 tcaatataaa acttactaat agcatataag ctatttgaga aggtctaagt attactattt
    14761 gtctccaatc tatatcagaa gtttttacat taaagtagtt tccgattgtt agagaagtaa
    14821 taaaggttaa agcaaaaaat aatattgtta ataatggcca ttttattccc atttctttaa
    14881 agcttaggta tctataaatg aagtcttgtt ttgcactttg tggatagatt gtagaaataa
    14941 aaaatagacc tacaatagct ggtgatgtat acgcaagata aaaaatgatt tctccaagta
    15001 tcgtatttgt aattccgagt aatgcaggtg taagactgaa tatccagttc aatactagag
    15061 taagtgtaaa gaaatagatt aaatttttgt ttgtattaaa tggggtgtta tatagttgca
    15121 ttttttcact atcctttcgt tttataatgg aatttgaatt tttattgatt gtttatttat
    15181 ttctatatgt attgagttat cgtttaatgt tagttattga ttataataat atcctattct
    15241 tattttgatt aattgttttt acactaaata taatcaatta atatttaata ttgaacatat
    15301 tatattattt ttaattatta aataggtcaa taaatttgca attagtgata ttaataaagt
    15361 actttacatt tttattaaat atttagcttt tctttaatat tttttatata acaacgattt
    15421 ataataactt tttcgttgtt tcttaaagta gcttgcatac gattatgtga tattactttt
    15481 atacttttta tgtagtccat atttaaaatc atggatttgg atattcgtga gaaggatgta
    15541 ttagctaata attttgataa tttatatatt tttaaatcaa tttcaaatac atcatcttca
    15601 taataagcaa atgttttatt tccaactgtc tcaaagtata aaatatcaca gacattgagc
    15661 ttataaatct ctcctgattt tttacccttt atatcaagta atggtgtact caataatgaa
    15721 attatttttt caatgttatt atctagagta gaacatttta tggttatttc aatatcatcg
    15781 caattaatgc cttgttcgac atgtattttc attaatcttc acctcatttt attactgtca
    15841 ttagaatagc attatatctt attgagtcca actagatgcg ttctaaattt caaattaagt
    15901 atagtgaaat tcaaaaatga aacggtgaaa ttcaaatttg aggtggtaaa attcaaaaaa
    15961 atactatgga tatttgggta aattatccaa atatcctagt atttttcata gaatatatct
    16021 aactatttta caaatagact tattttatga cttacatata ttgtcaaatt acttcttaaa
    16081 tagcaaaata tttttggata gtgtatcaat actgtttgat aaagtattta atttaccctc
    16141 aattctaacc aacaaataca tggacagagc tataggaaaa ccaacattgg ttattaacag
    16201 ctgtaaacta tcatccataa aattagcccc ctattcttat aactaaatca taaggagtgg
    16261 tatttgtttc cacaactttg gcttcaacaa cagaagctag gtcaaatcct ccaactgaaa
    16321 aaatattttt agatactatc aaatccatga cagattttat attttcttca gttaaatcat
    16381 cctttggatt ttctatagaa aaagaaactt gtttatcctt tacattttta aaactcatga
    16441 ctaatttttt cttaatttcc atataattac ctccgatata ataatttata ttttaattgt
    16501 ttgattgtta atattaattg tttatactag ttattgctga tatagaaaat gtttaattta
    16561 tttcaattaa ttagattgaa gtagggtagc atttaaaata gctaacatgt ttaagttgaa
    16621 atacgtttaa tatattaatt tttaaagtat caatatatta aagttataac tcaaattgaa
    16681 attataacct gccaaatcta cagcgtatta agtgcgttac ataactaaat cttttatata
    16741 aagagttaat tttttaattt gcaaatcaga ttatgtttaa aagacactat ttagatagtg
    16801 atgtgctatc taattttaaa atatctacaa tagggttctc ttgaagactt gctattgctt
    16861 ctgcaacagc ataaacatcc tcatttaatg cctctgcttt tacgtttgca aatgatttac
    16921 ttttaataac tgccttaccc ttatcgtcaa aaccagcatc caattttagt tttatactag
    16981 ataaattttt tgttgaaaca acttccataa aattacctcc taaaaattta atttcaaaga
    17041 tttgtcgact ttctttgtta gctataatac tatcatctaa gataatatcg gagttaaaca
    17101 gtaatttcta aagatttttt gtgtaaattt ttataggaaa aattttcctt atgaacaata
    17161 ataagtttac aaattattca tgaagatatg tttttagcgt tttgaaatag tatttgtata
    17221 tattcctaat ttagttggga tagttttgta tagaatttta tatgtataga gttaagtgtt
    17281 agaggttaaa gtttgtataa aattaaatgt taaagtctat gttaaaaatt ttattaaatt
    17341 taatctgtaa ataaaaaagt taaaagtcca atattaacat tttactttta aaccattaaa
    17401 atctttatta aagctaacaa agttataagt tataagaaat tttattaaag ttaatacaga
    17461 aattaatttt aaagctaaga aattttttgt taggtccaat aaaactaata aatatagttt
    17521 agtcaataat gtattaaaca aaaactttgt taagtctata aaaatatatt aaaggaggtg
    17581 taatttatga agataaaagt ggaagatata ccagttcaat ttcatgcaat ggtagaaata
    17641 atcggaattg ataagttcat agaggttacc aagttgtatg gaggaacaaa tacttatata
    17701 cctacgtata atggactttt tagatatgct agaaataggg aaatagtaaa acaatttaat
    17761 ggagtcaact acaatgaatt ggctattaga tataatatgt gtgtaagcaa tattaagcga
    17821 atactcaatg aaaatagtgt ttaagtataa tgaaaatagt tttattaaat gtaagtgtaa
    17881 tgataaagaa gatgttttat aaagtttttt tagacaaatt aactaaattt actacttatt
    17941 aatttaaata cattgtatat tctgtaaaaa aatttgttag atactgaaga tacattgaga
    18001 atacaaagaa aatagtcgaa aatatttgtt ggctaaaaaa tagataaaat gatacaattg
    18061 cttgtataaa agggaaaaat aaataaataa actatataaa taaagaaata tcaattaata
    18121 tgacataata tatttataat attaggaata taaatagata ttttaaggaa ttagttgtga
    18181 aattagaaaa tattaaaaaa aatatatatt tatatttaaa aaaatttgtt atttatataa
    18241 aaatacttat ctagttacta gcaattatta gtagaaatgg gggaaataga gatgaataat
    18301 ggattttaca aattaaattc agcagtattg aactttgata ttaagagtga tgttaacaag
    18361 ataagattta atcattatat aatgagaaaa gaaaacatac aaagacttca aggaactcta
    18421 ccaagaggac aattttatat gacagtgaga agagctgcta cagatttaca actttctatt
    18481 tctactataa gtaggcttgt aagagagttt ttggatttgg ggataataag attagtgtct
    18541 aagggtgtaa aaggtaatcg ttctgtatat agttatgtaa gtatagagtt taatggaagt
    18601 ttatttgatg atagcaaaca tatggaactt gattgtgtat gtaatgatga atttgttgat
    18661 aatagtgaca tagagcttga ttgcaatagt aaagataatt cttttaatgg agatataaat
    18721 gtagaaactg agtattgtag taatgagtat ttgtttaata atgatgtaga gcataattat
    18781 gacaatttct ataacaaaga tactataaag aataaatcca tttttaatat tagcactatc
    18841 aaaaaatata tcaatgaatc ttttgataca aataaatcag aaaattctaa cttacaacaa
    18901 gaatcaataa attttaactt taatgataca tgtaatgcaa tagataaaag aacgaataat
    18961 agtataaaaa gtaatcaaaa taaaaaggct atgaaattga gtgacatcaa tgttttaagt
    19021 gataaagata ttacaaataa aaagaattac tttaatcata ctcatggcat acaaaaaggc
    19081 actaaatctg taacaaagaa aaaagaatcc ttaaataaat ttaataaaaa aaatatacag
    19141 caaagcatta taaatatgct aaataaaaaa tcaggtaaga attttaaaat cgattctcca
    19201 ataagcataa aacttatcaa tgaaaggcta aaggagggct atgtcttaga agatttctac
    19261 aaagttattg aggtaaaagt ggctaagtgg aaaaacacca taatggaaat gtatataaga
    19321 ccagaaacct tgtttagcta taagtttgag tcctatgtaa atgaaaacat atctatgaaa
    19381 agtcaaagta atattcaccc gtgtaaagaa ggcaatagta atgatagcag taattatata
    19441 cattctaatt atacaacgta tgatgacagt gtgcgcgaga gaaaaaaata tgaaaatgta
    19501 agatactgga atcctatatg tgaaatttaa ttagtctaag aatgtattta tgataaataa
    19561 gatgttaata gcgaatagtg agctactgca aatgatatat caataactag tagtgaatta
    19621 tattgaatga tatatcaata attggtagta agttatatca aataagatat tactatcgag
    19681 tagtgaatta tattgaacaa tatatcaata attggtagta agttatatca aataagatat
    19741 tactattgag tagtgaatta tattgaacaa tatatcaata attggtagta aagtatatca
    19801 aataagatat tgctattatg tagtaaatta tataaaacaa tgtatcagaa taaagagtat
    19861 attaataatt tgtagtatat catgacgaat aatatagtca taacaaatag tgtgtcataa
    19921 caaataatgt atttataatt gataatatat ttaaaaatca aaatataaaa acttatgagg
    19981 aaagtgagat taatttatat gggtaaatca aaagatgttg aaatagagaa gtctatttta
    20041 ggcacaattc ttttagacaa taaattaagc tataagctag atgaattaaa tgaaaacatg
    20101 tttatgaatg atatatgtct tgagatattt aaaattatga aagaacttaa aaaagaaaac
    20161 atagtgattg atgtggccac tgtgaaatca aagatagata aaaaatctct agcaataaaa
    20221 acgagtgatg tgactaatat aattacttgg ggtcagaatt ttgggcttga tggtcatatt
    20281 aaaattttaa aagaaaattt agctagaaga agcataaatc aaacctgcca aaacctttta
    20341 cacagcctaa atcttggaga aaatatcgat acttgtattt acaaatttga aagtaatata
    20401 aaggagattt tggacaagga tacttatgaa aatgatgatg taaatagtat agcagggaag
    20461 gtgcttgatt ttttagaaaa caaaaaagat attggattta aatttggtat aaaattactt
    20521 gataccacta taggaggtct ttttaaagga gagttaacta caatagcagc taaatcaggg
    20581 gttggtaaga ctgctttggc actttaaata atgttaaatt catttaagca aggaaaaaag
    20641 actttattta taagtagaga aatgaccagt gagcaagtat ttatgagaaa tatatgcaga
    20701 gtaactggtg taagtacaag gaatatgaaa tctaaagaaa tagatgaaaa tgactggaaa
    20761 cttattgtaa atgcgatagg tgatttaagc gaaaataact taatttatat caatgataag
    20821 gtagatacaa ttagtgcaat tagaaagagg ataaggcagg taaaaccaga tttattgata
    20881 gtggattatg tgcaactcct tacatctcag aaaagcatgg ataaaagaga aagggaggta
    20941 gctacttttt cgagggaact caaaaatatg actttagatt ttaatatacc tgtaattcaa
    21001 ttaagtcagt taaatgatga aatgaaagat tcaagacctt atggagatag acctatgagg
    21061 gatagtaaag ccatctacca cgatagcaac aatgtggttt atatacatca attaaagggg
    21121 agtgattatg aagaagcagt gagagatatt ggagaaagtg aggaggcagt tagagcatca
    21181 gaatacaggg gaattaagat ggttgattta atagttgcga agtgtagaga tggacagact
    21241 aggcataagc atttttgtta ctttggggat aagttacatt ttcaagagct aaattattag
    21301 gtcaataaag tagtaataac aagcttatta aaagtcggga aatagcaaat agattataaa
    21361 atatgaggtg aaaatatgtt ggaattaaca aaaattatag aattacttga agaacacaag
    21421 atagaagtaa gtattccaaa ggagttacat aagtatagtt tattggaata ccttgaattg
    21481 tgtcaacaaa gtgggggaac atatttttat attaagacac tggaaggggc tttaaaagag
    21541 gctagaaatg agcttattat taaattgttt aatgggaaga attatatgga attagcgagg
    21601 atgtttgaaa tgagtgttat taatgtcagg catattgtta gaaaaaaata gatatttttg
    21661 gagaatacaa gtgtgatata cttctgaaat agaagttatt ataattgaaa taaaattttg
    21721 gttaatagat taaagttgtg tataagttta agattataga ggagtataag tgtgattaac
    21781 agcatgattt tgtgaataaa attgaggata tatatgagct aaatgtgtga ttaataaggt
    21841 aaggttgtgt ataagattat gagtatgtaa aaggatagtc tataattaat agttcagagt
    21901 tgtgtgtttg gttaagatta tataagaatt taatagatta aactatacta ataaggtaaa
    21961 atcgtgtgtt tagttaaagc ttattataaa atcttgatac atgattaatg atgtaatatg
    22021 tgaataaagt taagattata ataggtttaa attgtgacta atagaataaa attgtgaata
    22081 aagttaagat tgtaataggt ttaaattgtg actaatagag taagagtgta aacagagaaa
    22141 aacttcaata tgagcttaac ttgtgattaa tagagtaggg tgtaacaaaa gaaaaatttc
    22201 aatatgagct taacttgtga ttaatagagt agggtgtaac aaaagaaaaa tttcaatatg
    22261 ggcttaactt gtgattaata gagtaaggtg taactaaaga aaaacttcaa tatgagctta
    22321 atctgtgatt aatagagtaa ggtgtaaata gaaataaact tcaatatgag cttaacttgt
    22381 gaataataga ataagattgt gaataaagtt atagttttag atgggtatta acctgtgact
    22441 aatagaatat gattaggtgt aaaattatag agttgtataa gaaaatatta tagatatatt
    22501 aaatattaga taataaatat ttatatagat acacaaaaga aaaggcgata tgaagttaaa
    22561 cttaatgact tcatatcgtt ttttatttga aattcctctg tatttagagt gtgattttta
    22621 atattgaggt tttatgtgat taagtcacag ataaaaagta agtatcctga tataattctt
    22681 atagattaat aatattgggg aggttattat gtctaataaa aaaaagaagt atgcaaatat
    22741 tttagcaaat gagtgcgttg cttgtggtag ctgtataaaa gcatgtccaa gaagtgctat
    22801 ctcagttcca tgtggcatta gtgcaaaaat taatagagat ttatgtgttg gatgtgggat
    22861 ttgtgaaaag atatgcccag catctgtaat tgagattata acaatattaa aagaggaaga
    22921 aggtaagtgt catgaataat aaaaaacatt ggtatgacta tttatggatt ttttcttcag
    22981 tatatttgat attgggattt gtaaatatta ttttcgcttg gattggacta atttgtttct
    23041 ttgttccact agctatttct attgttaagg gaaataaagc ttattgtaat aagtattgtg
    23101 gaagagggca actgtttaat ttgcttggaa ataaattgaa gctgtcaaga aatagagata
    23161 ttcctaaatt tattagaagt aagtggttta gatatgggtt tttgacattt tttatggtaa
    23221 tgtttgccaa tatgcttttt tcaacttatt tagtatttga aggaagtaga aattttaaag
    23281 aagtagtgac tttgttgtgg actataaaaa tgccttggca atgggcgtat catggaactt
    23341 tagtttctcc atgggtggta cagtttgcat ttggttttta tagtgttatg ctcacatcaa
    23401 ctgttcttgg tttgattact atgatgttgt ttaagcctcg ttcatggtgt gtttattgcc
    23461 ctatgggtac tatgactcag atgatatgta aggcaaagag taagtctgta tagaaagaat
    23521 ttatgtgata tttaagagtt tgttcaagta gagatgagta gtaggtttga atatatgaca
    23581 aatatatgag tataggggca tagatttagt ttgtgtaagg taaactgatt tatgcttctt
    23641 ttttatgtct tagattattg gctaagtgga aataattaat ttagatatta agaattattc
    23701 agttatttga agaaaatata ataattaaca ttatatgaat ggatattcat tacatataag
    23761 ccagttaata gcaagattta tttaatatga agtaggtcaa gtatttaaac taaataagat
    23821 catgacaatt aatataagta tagatataaa aagattaaaa gttataactt ataaaaggta
    23881 ttctatataa atagtatggg aaaaatagtc aaattaactt attagaaaca aaaaatatac
    23941 ataaaaaata aatgtaaata tcaaataagg tactatatca aaatagatat aagtatcttt
    24001 tttattattg aaattatgaa aatatatttg attttacaca aaaaagagtg cctcatgaac
    24061 taaaaagttc attttgagac acacttttac agtgtcaaag aacgaagttt atccttatcc
    24121 aatactttta taccaccacg agataaagac acaatacctt ctctagcaaa atatttcaac
    24181 attctcgata caacttctct ggcactaccc atatacttag caatttgttc atgagtcata
    24241 tttatagtgc tactaccatt tttagctatc tcatcaatta aaaaagatgc caaacgctta
    24301 tcaaaactca taaaaagaat ttgttgcata gcccacataa catcagaaaa acgttcagta
    24361 gctaatttat atgaaaataa ctctgcatga atattttctg tagataattt tgcaaaaaca
    24421 gaggaactaa tctgtataat atcgcagtcc gtttcagcat ctacatagac atcaaaagtt
    24481 attgttttta gaatacagga agctgataaa atacatacat ctccatcatc aagtcggaac
    24541 agtgtaacct ctcgaccttc atctgaaagt atgtatgtgc gtattgttcc agactttata
    24601 atcaaaatac caatacagtc actatcacca caatgtacgg gattcccttt tttgtaatgc
    24661 gaggtgtttg ctgaagaaat taataattct ttttgtaagt cagtaagttt atcccaaaaa
    24721 ggcaagctat gtgtaagtat atcaatttca ttttgtgaaa tcataccata tctcctttct
    24781 taaattgtta taatcccatg atacaagtga atagataaat tgacaagtag tttgttaaga
    24841 ggattattta gtcagatagt aatagtatga tttatgtaga aaaaatatga taatatatat
    24901 tcttgctatt aatgaaaaag tgatatattt ttgatgatag tagatgtgta caactattgc
    24961 atacagttta tttttaaaag attaagttcc ttatgtaacc ttatagacat aaaacctgca
    25021 attatcgtag ctatcaaatc agcgattggt tgtgcatata agataccatt tatcccataa
    25081 aataatggta ggtaatccca tagataacaa tgcttttggg attggggcag accctaataa
    25141 ttcaattctt ttgttacttt ctttcatatt caacacttcc cttctattga cattaagtct
    25201 aaattataat actataaatt atagtgaata tggaaaatag tactataatt tatagtattt
    25261 gtcaatagta agaaaactaa aggagatgat ttttatggct acaatagact taattgtatt
    25321 gggaatagta aagcaacaac ccaagagtgc ttatgatatt caaaaggaag tggaatacag
    25381 gaatatatca cgttgggtta aaataagtac accatcaatt tataaaaaag taattcaatt
    25441 agaacaaaag ggatatattg aaagtaaaac tgtgaagaat ggaaaaatgc cagaaaaagc
    25501 agtttattca ttaactgaat taggtgagac acatttctta gacttaatgt ttgatacatc
    25561 tagtaaacca ataaatattc ttttagattt taatgctgtt atagtaaatt taaatagcct
    25621 tacaccagaa aaacagaaat tatgtctcaa taatattgaa gagaatgttc aagagttaaa
    25681 gaaaactatt gaggaaaata taagtataaa gacagaagta ccagagacag caaaagcagt
    25741 gttggagcaa cagttggtac tagcacaatc attggaaaca tggataaatt ctttaaaaga
    25801 taatatgggg aattagaatt agagaagcta acttaataat cattaaacaa aaaccagcat
    25861 atttaataat gagatattag aaaaaatgaa acaaatgata taaactgaaa agtatattta
    25921 aaataaagct cacttaattg agggtgaatg ttaaactttc taagtgtaag gaggcattat
    25981 tcaggaatct actcgactac tgttattgtt aatggtgtag aatcaggtaa ccttaattct
    26041 ggattaggca tatgatttga ttttgaatta ggtgtataat tttgttaata agtagaaaaa
    26101 ttggaatctg tagtcgtggt tgtaaatttc actttatcat tgattaacat agctgttgat
    26161 agaatatttc aaaaattaga atttggagag tgagcactat ggaagaaaaa tacatcttat
    26221 ctgaccgagg gaaagtatat tattggataa aagaaagtaa tagtgtgttt gcaaagaact
    26281 taattttttt gccaggacta acagcaaatc atcatctttt tgatttccaa atacagtact
    26341 tttcaaaaaa tcataatgta ttggtatggg acgctcctgc tcatggaaaa tcaagacctt
    26401 acttagattt ctcatattct aacttggtag aagaattgaa agtaatttta gataaacaaa
    26461 atatgaaaag ggttgtactt atagggcaat cggcaggggg atttgtggca cagtccttta
    26521 ttgtaaaata tccaaatatg gtagaaggga taatgatgat tggaacttgt ccatatggaa
    26581 ttgactatta tagtaagtta gatattttct ggttaaaaca gactgaatgg atgttaaagt
    26641 tgttcccaga tagattgttg cgaaacataa tatctaagat gtgtggagct acgtctgaag
    26701 gcagaaggag tatgctgaaa atgttagaag gatacgaaaa aaaagaactg tgtcatctca
    26761 tgtatctggg atttgctggg cttataccag aaattcaaga cttaaatata cattgtccag
    26821 tttgtttagt agttggagat aaagacaaga ctggtaaagt aagaaaatat aatgagcagt
    26881 ggcacaataa gactggatat ccattgcaca ttataaaaaa tgcttcacat aatgtaaatg
    26941 tcgataaacc taatgaacta aatcagatta tggatatatt tattaagcaa ttataatata
    27001 taaaaattac atacccatta ggtaaattat gtagtatatg tgtttataga tggttataga
    27061 aataaatatg taataaacta cttaacactt aaaaagtaga cttgtctagt gcataataca
    27121 aagcatgata tatattttta agcgaaatac attaaaagaa gaactagtgg agataaaaca
    27181 gatattagat attataaatg attaacttaa aagtactata agtgtagaga aagatgactc
    27241 atacgttatt aagggattgt aaatgattag tttaaaagta ctatcgtata ggattctgca
    27301 tatttagacc ctagtaaaga tagttaaagt agtttaaaaa ctaaaaaatt atataaagga
    27361 attaggtata agaaaatgat tgagagtata ccagcaaaac aaattttaca aaaagtgaaa
    27421 tttgacaata caagatggtt tggaattgac tataatatga acctctatcg tggttgttct
    27481 catggatgta tatattgtga tagccgtagc acaatataca acatagagaa ttttgacaaa
    27541 gttagatata aagaaaatgt gatagaaatt ttaagtaaag aattgcgttc aaaaagaaaa
    27601 aagggggtag taggcattgg agctatgtct gacacatata atccttttga aaagcaactt
    27661 tgtattacaa agaaagcact agatttaatt tcggaaaatc attttggagt gtctattgat
    27721 actaaaagta gtttggtggt tagagatata ccgattttgc agaagattaa aaaaaataac
    27781 tctgctattg taaagttgac tataaccact gcaaatgatg aattatcaaa gaaaatagaa
    27841 ccttatgtaa atccaagttc tgtacgattt gaggcagtga aagaacttaa tgattctgga
    27901 attttttgtg gcatattatt gactcctatg cttccatttt taactgataa taaagatgaa
    27961 atacgagcaa ttgtagaaaa agcacacaag gctaatgcaa aatttattta ttgcatgtat
    28021 ggtgtaacaa tgcgtagtgg tcagagagag tttttttatg agcatttaag agatatatca
    28081 ccaaaattgg tacttaaata tcagaaaaca tatggtttaa actatgtatg tactgtacaa
    28141 aataaagatg gttgtgagaa gttgttaaga gaagaatgtg cacgttatgg tattttgacg
    28201 gatatgaaag agattataaa agcgtacaag aggtcggagt catatactca aataaaattt
    28261 ttttaagata agctttggat aagatataat agtgatgatt gatgtgttga tactctaggt
    28321 aattactaga gctgacatta taattgaata ttagtattga tattctaggt aattgttagt
    28381 gatgatatta tagatgaata ttaatattga tactctaggt aattgctaga gctaatatta
    28441 tagatgaata ttag
//
LOCUS       DAFNNB010000033        26720 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0033, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000033 DAFNNB010000000
VERSION     DAFNNB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0033"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..267)
                     /locus_tag="L3M60_001289"
     CDS             complement(<1..267)
                     /locus_tag="L3M60_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225972.1"
                     /translation="MDFGNINLILIGIIVIIGTTIIYLIKPKTAFCSKKYFNKLESIY
                     GNIDKKKTVKLELLYRYVTGLEYIAIGLFTRRLDITIITIILVST"
     gene            complement(645..845)
                     /locus_tag="L3M60_001290"
     CDS             complement(645..845)
                     /locus_tag="L3M60_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225973.1"
                     /translation="MCFKTYILKRVLNNKHLEITRSFDLDNLICTATEILKDNEIDLI
                     ITDEKNNVVWRNEKSTPQQENA"
     gene            complement(864..1277)
                     /locus_tag="L3M60_001291"
     CDS             complement(864..1277)
                     /locus_tag="L3M60_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6225974.1"
                     /translation="MKVKSKRSFIVGIIVCMLCCASLIIYCILKEKRFLISSFLLIAI
                     AIFNFCNAFSRKGIVEELHDSTDERDLYLTMKTSHILVKIMNYTLFTFTFLFIIAYSA
                     WKNQSLIVIAITLCVIEIFLFVAYLLINIFLEKKE"
     gene            complement(1261..1494)
                     /locus_tag="L3M60_001292"
     CDS             complement(1261..1494)
                     /locus_tag="L3M60_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6225975.1"
                     /translation="MKTKVREFRTNMGLTQQQLADLVHVSNRTIISIEKEQYSPSLML
                     AYRMAQVFGTTIEELCCLKENKEMEDKKHESKE"
     gene            1606..1773
                     /locus_tag="L3M60_001293"
                     /pseudo
     CDS             1606..1773
                     /locus_tag="L3M60_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015984867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
     gene            2359..3051
                     /locus_tag="L3M60_001294"
     CDS             2359..3051
                     /locus_tag="L3M60_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6225976.1"
                     /translation="MYNILVVDDDKEIVDSIEIYLKSEGFKIYKAYDGMKALEIITEN
                     DIHLILMDIMMPKLDGIKATVKIREERNIPIILISAKSEDTDKIMGLNIGADDYITKP
                     FNLLELIARVKSNLRRYVTLGNYEDTCKEILKSGALELNTLTKEVKIDGENIKMTPIE
                     YKIIELLLENKGRVFSIDEIYEKVWNEESFNSENTVAVHIRRIREKIEINPKEPRFLK
                     VVWGVGYKIEKF"
     gene            3247..5319
                     /locus_tag="L3M60_001295"
     CDS             3247..5319
                     /locus_tag="L3M60_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6225977.1"
                     /translation="MEKYRALKLVSLIIISISFPLTILLATIGLAEPKYNIQKTSTKS
                     GIFNISNDFYDSNIFDEGTVASLLSNFKISVDSQLTSEEKSKFQTKSKSIDLSSNDSE
                     YDKIMINKQASEEYLGYLKNIKVYVINTYTGVFYTNTSYDNLNDFKQETKNYCNIEII
                     NNVGKSSYIKTINGEKFELNNLSMELNGVDEAFEAYVSFPEEPTMEDGIVYTNFQIFK
                     QATEKVRLFFVASLSLAIICLLFIILYSRIKCEVLKRHNVFLLIYSKIPMEFNIILVS
                     ISIAILTIYALAVSYHIDVIVLVSLSLLIVSNNIFFINVQLSYLENKMYIFKTSIIVR
                     VILWLIRTVREVSNAIKKVSLARKVIVIVIISLALIMVISARSISMGLLFSICIFLGF
                     TIYITKRLSYLSYVIEGTERIKKGELDYKIKITGNDNFTSLAENINNIGEGLDRAIEE
                     QVKSERMKSDLITNVSHDLKTPLTSIINYVDLIKKEESIQPEYINDYINVLESKSKRL
                     KLLIEDLFEASRVSSGNIELQISKIDLVQLLRQSIGELEEKLSKNNLYLKLNVPNDKV
                     YIWADGRRMYRVFENLLSNIAKYSLEETRVYIDVYDYGENVKVTMKNISSYELNFDPS
                     EIMERFKRADESRNTEGSGLGLAIARDLVDLQGGKFYIEIDGDLFKANLEFQKYEEEV
                     TNMEKDGI"
     gene            complement(5512..5835)
                     /locus_tag="L3M60_001296"
     CDS             complement(5512..5835)
                     /locus_tag="L3M60_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HBN6225978.1"
                     /translation="MNSNYIFFIIIGMYIVTYLPRVLPMLIFSKKEMPDFLKKIMKFI
                     PVAILTSLTAKDVFFNGDSLDLSIANPKIISFMIVLLVAYKFKSIGISIITGVVSIYL
                     FGIIM"
     gene            complement(5828..6529)
                     /locus_tag="L3M60_001297"
     CDS             complement(5828..6529)
                     /locus_tag="L3M60_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HBN6225979.1"
                     /translation="MERTQQLRESFIASIPIFLGYIPIGIVGGILLQKSGLSPFQIAL
                     MVTLVFGGSSQFIAASMISTGASVTSIVLTTFIVNLRHFLMSSNLNMYIKNKSPKFIL
                     PFCHTITDETFAVNYEKFTAGNWSDRNAIYLNFFCLISSVLANFIGAFLGESISIDSS
                     ISGFILTSMFIALIVSQIKNKIYIVVFISSIIISVILYALFKSNLVIIVASILASLIG
                     FFIEESYCKKEDSYE"
     gene            complement(6807..8192)
                     /locus_tag="L3M60_001298"
     CDS             complement(6807..8192)
                     /locus_tag="L3M60_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN6225980.1"
                     /translation="MPINSFENYPMNWKPKRPSKGQILYKALAEQLEQDINNGFLLPG
                     TKLPPQRELADFLDVNVSTISRAFKICEKKGLISGVTGSGTFVSYDTRSNLFLMSSNN
                     KITFIEMGTMNPDFTLEEMNTLFKHIVKEIDFKTIFQYGQRDGAKWQKEAIAKLIYKA
                     GLETTADSLLPASGGQNAIVAILAGLFQHGDRIGVDPLTYPGIKTAAKMLGVQLIPIK
                     QEHNEISEEGLLYACKNENIKGLYIIPDYQNPTTHIMSQNGRKMIANIASKYNLIVIE
                     DAIHSLLNETHLNPVASYLPNQTIYITSLSKIIAPSLRLAYISTPKQYRESLSDALYN
                     INLSQSYFLTEIAYRMITSGEADKLINARRKSARRRNKIINQYLSGYNLLGNEECIFR
                     WLILPEGIMAEKFEIQALKEGVQVYASERFAVGKEKPISAIRIAVCATESIEELKAGL
                     SILKRLLEEKK"
     gene            complement(8296..9534)
                     /locus_tag="L3M60_001299"
     CDS             complement(8296..9534)
                     /locus_tag="L3M60_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBN6225981.1"
                     /translation="MELFIQVIKFNIYISPLILFFILFTKKISKYTFKFNYFICLLII
                     VRMLFISKINLNILNINNSTINIYKHNINNYIPYSQDIILDIFMYIWILGIILISFHY
                     LRTNLKFYNYVENFKKEILEIDITSILDEQVKELNINKKLKIYKVKGVYSPAIIGLTC
                     CEIIIPEKKYNNKELRFIFKHELIHYKRKDNLFKLLISIICIIHWFNPILYLLKKYFN
                     ELCELSCDEIVIDKCSISEIKEYAYIILDTIKYQKTLKYSSFVSQFLNKKNITLKKRL
                     NYMFNQDKKKINLGFKLLLTFIIIGSIFTINSNFKQNEDIEDTTGKINYNKNGETYGT
                     DIIDSNGNSIEPELIQAIGRGKKVGYVKKRDLYDCDNQPNTPEEAVLYTRKRNFNIFK
                     ITIPLYDKEGVKIIGRFEIN"
     gene            complement(9547..9924)
                     /locus_tag="L3M60_001300"
     CDS             complement(9547..9924)
                     /locus_tag="L3M60_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6225982.1"
                     /translation="MIILKKIPKSELLVMNFIWDKGIDKIPAREVANYMENSHGWTKG
                     TTGKVLSRLSDKGFLEFEKDGRNTIYTIKVKHEEYLKFETKDFLELIHKNSIHSFISS
                     LGCENEMSDDDIKELEQWLKKMK"
     gene            complement(10418..10840)
                     /locus_tag="L3M60_001301"
     CDS             complement(10418..10840)
                     /locus_tag="L3M60_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HBN6225983.1"
                     /translation="MNTFKIEVLLKYSAKDIFNIFTKSARLNFPNFNEKKAVGTFVQQ
                     KNNKRFKVEITNFEKNRVYEIKTSNKRESIITRYELIHIDLENTKLIFTENMSERNVF
                     GKINSVLVRILFGKRQPEEFNKFIKNLEMELENNSVNL"
     gene            11317..14220
                     /locus_tag="L3M60_001302"
     CDS             11317..14220
                     /locus_tag="L3M60_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6225984.1"
                     /translation="MELRDKLVNIINTENKKDPLTDVQIAKFLSTTRENITNLRKELN
                     ISNSRQRRYPYLKSAISTILQKNKNISISEITRELMTEGFNISRRVVEELLPKESLEM
                     EVVEETEEESNDPFETLIGNKGSLRNAVEQAKSAILYPPKGLPTLIVGESGVGKSLFS
                     RHMYEFAKQKKIIKESSNFVVFNCADYSDNPQLLLSLLFGYKKGAFTGAEYDAPGLVE
                     EADEGVLFLDEIHRLPPKGQEILFSILDRGKFRRLGETNAERKVSIMLIGATTENVET
                     NLLLTFRRRIPMLITLPPLHDRLLKEKIDLIYNIFQQECNRINAKIFVDKNVIEILAL
                     KKFSGNIGQLQNMIQVLCARAFMKFINSKDEDSESIVVVDINEVLKLKDSFKDAAFQE
                     VEYTEIRKYLKNAIFIPFNLEEALVKGNLLSDKYILPEDIYKTIEKKYYDLKSLDIED
                     IDIENILWTFILNRFADLKFNLDSSEDIFSMSDLNSFVNDSLVKLVKELMNDIVEKNI
                     NLEVNKNMFKYLAIHLEEAIKRIKLNQKIINVNLEKIKIDFSKEYEISKSFAIKIEES
                     IGIKIPDDEIGFITLYIKSALKNEAKKDKVGLIIISHGRIATETVNVVKELLGVKFPV
                     AIDMPLDEKPINIYNKAVELSKIIDQGKGILFLVDIGSLTNIGQIVNKRTNINTKTID
                     RVDLLMALEAARKVSMGEEELDEIFFSLLKDRMGYNYELEKHIDKSNAIVTLCLTGEG
                     TAKYISKTLEEKYDNTKCYQMSALDENLFCKIENIKETSNILAIVGTINPKVPGINFI
                     PYNKEVFKNLDIYLSKSKQQDDRPIKSYERMLDEDLVIFEPDIYFKKDLLEYVCSILI
                     NKEYVEKDYLDSVLHREEMLPTYSKGFIGVPHGDSSTVNSTRFVFVKLKNPIDWGVGN
                     VNFIFMPVFQANDKEIVKNVLEILKDEEFMNNANKCFNKDAFKKIIFDKFKHL"
     gene            14253..14699
                     /locus_tag="L3M60_001303"
     CDS             14253..14699
                     /locus_tag="L3M60_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6225985.1"
                     /translation="MNVDLISLGLESNDYESVIEELGSIMCKKEYVKDTYINAVLERE
                     KTLPTGLDIGEMCVAIPHTDSKHVNESNVAVGILKNPVKFNSMIDPKDRLNVELVFLL
                     AVKNPDSQVKLLKDLMSVFQNVKLLKNIKNASTKEEVAKLLDFIEI"
     gene            14820..15122
                     /locus_tag="L3M60_001304"
     CDS             14820..15122
                     /locus_tag="L3M60_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6225986.1"
                     /translation="MKQFNVLSVCGSGTVTSSMVAGKLKEVLGNKGISITTTEARPTE
                     ALNLAQSGRFDFITFTSPLQTGDYGIPTINAFACLTGIGEEAFFEEVLTVIEGIDK"
     gene            15301..16707
                     /locus_tag="L3M60_001305"
     CDS             15301..16707
                     /locus_tag="L3M60_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBN6225987.1"
                     /translation="MLEFMKSIIDTFGSAIIVPIIIFIIAKIFKVTTKKSFLSAVYAG
                     VALQGFTLILNSFTPIITPVINRMVESTGVNLPVFDVGWQATSLVAFSTSAGMIYLGL
                     GILLQTILFLIKWTDVFQPSDLWNNYSYMVWGAMVIGVTGNFPLGIACMVLLNLYSLL
                     ISELVAKRWSSYYRYPNCTIIAMHNVEASVFAVFADPIYNKLGLNKIKLNPKELEKKL
                     GFLGEPITLGLFLGMFIGILGNMTRITTMEAWGEIMKVGISTSAVMAIFPKVASMFAQ
                     AFAPITEAARKIMQKAGNREWYIAVNDAVGYGEPATLISGLILIPIMLVIAMVLPGNK
                     VLPVVDLLAIPYMVQGLVAIHNGNIPKVLVSGIIWFGLGLYVCTSTAPLFTDMATNIG
                     VAIPAGAMLITSFNILGKPLMGLVFFAFLSANPIYIGIAVGIYFALWALFRKNKASIL
                     DYLEKQALKNVEEEPVAV"
     gene            17052..18110
                     /locus_tag="L3M60_001306"
     CDS             17052..18110
                     /locus_tag="L3M60_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBN6225988.1"
                     /translation="MGNKMRASVLYNVGDVRYEMVDIPEITDTQVLVNVKYVGICGSD
                     LPRSMVSGLSGNTKYPLILGHEFSGEVVKIGEKVKHINVGDRVAVAPLVPCGECDYCN
                     EGNFGLCDDYNIIGTRVNGAFAEYVRVPEKHVLKLPDTLDYETAAGIEPATIAYHGIS
                     KSNIKAGDSVVVLGCGPIGQFSIQWARVFGASKVIAVDIFDEKLELSKFLGANYILNS
                     KEVNVIKEIKTITNGGADVVIETAGSRFTQEQSLFVAKKRGNIVFVGISHTELPLSAD
                     ATERILRGELTLKGSWNSYTSPYPGRAWTATLDFMGKGDIIFKPMISHKIGLNEVGDF
                     LSKMSKREINFNKILVEI"
     gene            18207..18893
                     /gene="araD"
                     /locus_tag="L3M60_001307"
     CDS             18207..18893
                     /gene="araD"
                     /locus_tag="L3M60_001307"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBN6225989.1"
                     /translation="MLENLKEEVLKANLELPKKKLITYTWGNVSGIDREKGLVVIKPS
                     GVEYSNMTVEDMVVVDLEGNVVDGKLRPSSDTPTHLVLYREFEELGGIVHTHSSWATI
                     WAQIGKPIPSCGTTHADYFYGSIPCTRKMTREEICNEYEKETGNVIIEEFMGRNPIHC
                     PGIIVNDHGPFTWGKDANEAVHNAVVLEEVAKMAYYTELMSPDNLMDKVLMNKHFSRK
                     HGKNAYYGQK"
     gene            18911..19558
                     /locus_tag="L3M60_001308"
     CDS             18911..19558
                     /locus_tag="L3M60_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HBN6225990.1"
                     /translation="MLESLKGKVVEIAIKAEHYGLCKEKTGNFSIRDKNTGYILITPS
                     GIGREELKVEHICVLDLDGNVIEFEKGIKPSSEVLMHIEIYKTRENVNAISHTHSLYA
                     TAFSVAKKPIIPIVYESVNYGGYVDIAPYERPGTLKLAKIVSKLLLDTDACLLERHGL
                     TTVGNDLAEALLKSRYVEDVAEIYYKSLVLNQFKELEVVDVDEFKSWKYPEEIKF"
     gene            complement(19731..21098)
                     /locus_tag="L3M60_001309"
     CDS             complement(19731..21098)
                     /locus_tag="L3M60_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HBN6225991.1"
                     /translation="MNLIDILNKVDAFIWGPPLLVLLVGTGILLTVKLGVVQITKLPR
                     ALKLIFSAENKGSGDVSSFAALCTALAATVGTGNIVGVATAIKAGGPGALFWMWIAAF
                     FGMATKYSEGVLAIKYRTKDKNGQVSGGPMYYIVNGMGEKWRPLAIFFAISGILVALL
                     GIGTFTQVNSITDAINNSFGIDPRITGVVLAVFVALVVFGGLKSISNVATKIVPFMAV
                     IYVVICGIILVSFWNKIPETFMLIIKSAFTPTAATGGFLGATMSLAIRNGIARGVFSN
                     ESGLGSAPIAAAAAKTEWPAEQGLISMTGTFIDTIIICTLTGFSLVISGVWCSDLNGA
                     VMTQAAFNGAIPTFGPILLTVSLTLFAFTTILGWSYYGERCFEFLFGVKGMNGYRTVF
                     VAMVLLGAFLKLEVVWIIADIVNGLMAIPNLIALLALSPIIVSETKKYFEHINSPENQ
                     IKKNA"
     regulatory      complement(21288..21376)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            21803..23923
                     /locus_tag="L3M60_001310"
     CDS             21803..23923
                     /locus_tag="L3M60_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBN6225992.1"
                     /translation="MGKVLVLAEKPSVGRDIAKVLGSKNEKNGYIEGPKYVVTWALGH
                     LVTLADPESYGERYKSWSLEDLPILPKHLKTVVIKKSGKQFNTVKSQMNRNDIDEIVI
                     ATDAGREGELVARWIIEKSQVKKPIKRLWISSSTDKAIKEGFAKLKSGKEYENLYYSA
                     IARAEADWIIGINATRALTTKYNAQLSCGRVQTPTLAMLLKREEEIRNFKPKEYYGLE
                     LIATKGNSDIKFIWNDKNNNSSTFSKEKIESTLKKVKGVDIKISEVNKSYKKKYSPAL
                     YDLTELQRDANKMFGFSAKETLSIMQKLYEHHKVLTYPRTDSRYLTSDIVGTLKDRIK
                     AVNISDYSRICNKLLKSNITANKSFVDNSKVTDHHAIIPTEQKVFMSDFSDKERKIYN
                     LVVKRFLAVLSPPFEYEQTTISATVEGEIFKAKGNKVINLGWKESYKDIEDEDEYESI
                     PNVLEKDVLKVKGLKVTSGKTNPPPYLNEGTLLTAMEKNGLGTVATRADIIEKLFNSF
                     LMEKKNKDIYITSKGRQLLDLVPIDLKTPELTASWEKKLLDISKGKLSKNTFINDMKD
                     YSKDIVLEVKNSDNKFKHDNLTKNRCPECGKFMLEVNGKKGKMLICEDRECNTRKTIS
                     QVTNSRCPNCHKKLELRGEGEGRIFTCSCGYREKLSSFNKRKQEEKQKASKKDVNQYL
                     KNQKKNQETFNNPFAEALAKLKNK"
     gene            24024..25277
                     /locus_tag="L3M60_001311"
     CDS             24024..25277
                     /locus_tag="L3M60_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliS"
                     /protein_id="HBN6225993.1"
                     /translation="MDLSSIFKHYCKECENTWNNSSVELFDNIETYSKDSQKKMEKEL
                     DKLINTISVHLERYPSDIVLRKMWVKKGEIFLQKTLEKENIFKLEKMDVEDRKKFLDI
                     TKQFIRDARKFDSDLPISDIMQAMRNVWISNALQVLFGKEVYYSKANFAYSMLYPYTD
                     NYLDNTSIDKNDKILFNNWLEKRLLGEHIKSNDYHESKVSQMIDYIESVYPREKFIEV
                     YESLLLIFKSQVKSLKQHGEENHLCKEDLLSISIEKGGSSVLVDGYLISGLMTKEEID
                     FCIGYGFLLQISDDLQDVKEDLKYNHKTIITEMAKEGNLDKVVNKLINFTIELIDSFK
                     INNKNKSVITMIKNDCLMLILFSVVYNAEFFSVGYIKEVERFIPYTIDYSLEIEEKMK
                     EKFKSIDVLNNENEYKEMIDIICAE"
     gene            complement(25432..26493)
                     /gene="ytvI"
                     /locus_tag="L3M60_001312"
     CDS             complement(25432..26493)
                     /gene="ytvI"
                     /locus_tag="L3M60_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HBN6225994.1"
                     /translation="MDIQKQKNFIIKFTYVVLITSIVYIVLKFLLPLLMPFVISFIIA
                     SILRPIIKLITNNTNLNRTLISIVILLAFYGLCIFLLISFGVKIFASVSDVFFRLPEI
                     YKSSIQPTLNTLFSKIDASTPNVNLALMLGWDNISQSMMSLVASISTNALNAIASIAS
                     KTPAFMLKLIITLIASFFFTFDYQKIVNFILKQFPEKSQFMIINIKNSSINALLKLLK
                     AYAILLSVTFIELLIGLTILKVENAFTISVIIALVDILPVLGTGSILTPWMIISLING
                     NINLAIGLLILYIIITVVRQILEPKVVGHQIGLYPLITLMCMFVGAQLFGIAGLFGFP
                     IAATIIKNLHDNGIITAFK"
BASE COUNT        10417 a         3093 c         4113 g         9097 t
ORIGIN      
        1 agtagatact aatattattg ttattattgt aatatctaat cttcttgtaa ataagcctat
       61 tgctatatac tcaagacctg ttacatagcg ataaagtaat tctagtttaa cagttttttt
      121 cttatctata tttccatata ttgattctaa tttattgaaa tattttttac tacaaaaagc
      181 agttttaggt ttaattaaat atattattgt agttcctatt ataactatta ttccaataag
      241 tataagattg atatttccaa aatccatagt agaaacctcc tgttagattt gattttctta
      301 atcaaagtat atcagtaaca caagtataaa gagtatctaa aatattacaa atattaacag
      361 gacgcataaa actatatagt ataagttgca ataataaaac taacgaaaat tatcatacta
      421 aaacataaga cacgttataa tcatgttaga gaaattcttg aatatacact aaaagtactc
      481 tttctatagg agtgcttctt tttttaaatt tattaatgta taaactatca agactgttac
      541 tcaaaaccct ttaaaattga tttaaatagc tctttttaac aacataattt atatgatata
      601 ataaaaaata gaagtgtatt cgcagtacac ttctatagtt gtaattaagc attctcttgc
      661 tggggagtgc ttttttcatt tctccaaaca acattatttt tttcatctgt aattattaaa
      721 tcaatctcat tatctttcaa aatctctgtt gcagtacaaa tgagattatc taagtcgaaa
      781 cttcgagtta tctctagatg tttgttgttt agaactcttt taagtatata agttttaaaa
      841 cacatttgta tcacctcttg tatttactct tttttctcta aaaatatatt gataagtaaa
      901 tatgcaacaa ataaaaaaat ctcaatgaca catagtgtta tagctataac tataagtgat
      961 tgatttttcc atgcactata agctatgatg aaaagaaatg taaaagtaaa taatgtataa
     1021 ttcataattt taactaagat atgacttgtt ttcattgtta agtacaagtc tctttcatct
     1081 gtactatcgt gtaattcttc aacaatacct tttcttgaaa aagcattaca aaaattaaag
     1141 attgctattg caattaaaag aaatgatgaa attaggaatc ttttctcttt taaaatgcaa
     1201 taaataatta aactagcaca acataacata caaactataa tacccactat aaaacttcgt
     1261 ttactcttta ctttcatgtt ttttatcctc catttcttta ttttccttta aacaacatag
     1321 ttcttcaata gttgtaccaa acacttgtgc catacgatag gcaagcataa gagaagggct
     1381 atattgctct ttttcaatag aaataattgt tctgtttgat acatggacta aatcagcgag
     1441 ttgttgctgt gttagaccca tatttgttcg aaattctcgt acttttgttt tcattccata
     1501 acctctttct gtatgaagtt aacttcatgt gaagttagct tcataatatc ctaaaatgag
     1561 taaaatgtca atcttattag ataagtatta taaaaaccta aaagaaaaaa atatctaagg
     1621 tttgaaacga aagaattttt gaagaacata cataaaaatt caatttcaag tttaatgtca
     1681 gcgttacatg gtgaatacga cgaagtggat gaagataaat taattgaaat cgaagaacat
     1741 tttaagagtt tgaaagaaga aaatgacagc tagatacata ataaataaaa tgaaggtatg
     1801 aaattataac tttttctacc ttcattttgt ttattattct gaacatgtta agaaaatgaa
     1861 atagatttgt tttatattta tataaatttt aatagaatat gcatagtata tagtagatag
     1921 tatgtataaa aatagaattt aaataagcag ctattattgc agcgaaataa atgtttctaa
     1981 gtatatttgg gtggaagaaa attagtgaaa tgtaattctt ccaccactct tccacccaaa
     2041 aaataaaaac tgttcaataa gaattaatat acattaattc agctgaatcc aatcaaaata
     2101 cttaaataaa agtaatctga aaacaacata attcaaatca atttattcaa ataaacctca
     2161 atttctctta aatcttaaga aatatttaag aattaggata atattttctt aagatttata
     2221 ttttattata gacttataag agaaagttac aaaaatgtaa gtcaaagtaa tataacaatt
     2281 tagtcattgt tgagaaagga agtgaaagat attgtataat atagataagt attattatac
     2341 aatagaggaa gtgaaaacat gtacaatata ttagtagtag acgatgataa agaaattgtt
     2401 gattcaatag aaatatatct taaatcagaa ggatttaaga tatataaagc atatgatgga
     2461 atgaaagctt tggaaataat aacagagaat gacattcatc taatactgat ggatataatg
     2521 atgcctaaat tagatggaat aaaagcaact gtaaaaatac gagaagaaag aaacattcca
     2581 ataattttaa tttcagcaaa aagtgaagat acagataaaa taatgggatt aaatattgga
     2641 gctgatgatt atataacaaa gccatttaat ctattagagc ttatagctag agttaaatca
     2701 aacttaagac gatatgtaac tttaggtaat tatgaagata catgtaaaga aattttaaaa
     2761 agtggagctt tggagttaaa tacactaaca aaagaagtta aaattgatgg tgaaaacata
     2821 aaaatgactc caattgagta taaaataatt gaacttcttt tagaaaataa aggacgagtt
     2881 ttctcaatag atgagatata cgaaaaagta tggaatgagg aaagttttaa ctcagaaaat
     2941 acagtagcag tacatatcag acgaataaga gaaaagattg aaataaatcc aaaagaacca
     3001 agatttttaa aggtggtgtg gggagttggt tataagattg aaaaatttta gtggaaaaat
     3061 tataaaagaa aagagtttaa tatttaattg cattttgatt ttagtcattt ttacatttac
     3121 acaaatgtta ggaattttaa tatgttttaa ttttttctct gagagtttaa tttactctat
     3181 ggggattatg cttttcttaa actttatttt atataaaata ctgacaatgt agatgaggtg
     3241 aacaacttgg aaaaatatag agcattaaag ttagtttcat taatcataat atctatctca
     3301 tttccattga ctatattgtt ggcaacaata ggacttgctg agcctaaata caatatacaa
     3361 aaaacttcta caaagagtgg gatttttaat atatctaatg atttttatga tagtaatata
     3421 tttgatgaag gaacagtggc aagcttatta tccaatttta agatatctgt ggatagtcag
     3481 ttaactagtg aagaaaaaag taaattccaa acaaaaagta aaagtataga tttaagtagt
     3541 aatgattctg agtatgataa aataatgata aataaacaag cttctgaaga atatttaggt
     3601 tatctaaaaa atataaaagt gtatgttatt aatacatata caggagtatt ttatacaaat
     3661 acatcatatg ataatttaaa tgattttaaa caagaaacta agaactattg caatatagag
     3721 ataataaata atgtaggaaa gagctcgtat attaaaacaa taaatggaga aaaatttgaa
     3781 ctgaataatc tgagtatgga actaaatgga gtagatgaag cttttgaggc atatgtatct
     3841 tttccagaag aaccaactat ggaagatgga atagtataca ctaattttca aatatttaaa
     3901 caagctacag aaaaagtaag attatttttt gtagctagtt taagtttagc aattatatgt
     3961 ttattattta taattttata tagtagaata aaatgcgaag ttttaaaaag acataacgta
     4021 tttttactaa tatacagtaa gatacctatg gaatttaata taatactagt atctatttca
     4081 attgcaattt taactatata tgctcttgct gtatcttatc atattgatgt aattgtgtta
     4141 gtttctttat ctttactgat agtatcaaat aatatatttt ttataaatgt tcaactgagt
     4201 tatttagaaa ataaaatgta tatatttaag acaagtatta tagtaagagt gattttatgg
     4261 ttaataagaa ctgtaaggga ggtcagtaac gctataaaga aagtatcttt agcaagaaaa
     4321 gttattgtta tagtgataat atctttagct ttaatcatgg taattagtgc tcgaagtata
     4381 agtatgggat tactgttttc tatatgtatt tttttaggat ttacaatata tataactaaa
     4441 agactttcat atcttagtta cgtaatagaa ggtacagaaa gaataaaaaa gggagaatta
     4501 gattataaga taaaaattac tgggaatgat aattttacgt ctttagctga aaatataaat
     4561 aatattggag aaggtcttga tagagctata gaagagcaag taaaaagtga aagaatgaag
     4621 tctgacctta taacaaatgt atctcatgat ttgaaaactc cacttacttc tataataaat
     4681 tatgtagatt taattaaaaa agaagaatca atacaaccag agtatataaa tgattatata
     4741 aatgtgcttg agtcaaagtc aaaaagacta aaattattga tagaagattt atttgaagcg
     4801 agtagagtaa gtagtggcaa tatagaatta caaattagca agattgattt agtgcaacta
     4861 ttaagacaaa gtataggtga acttgaagaa aaattatcta aaaataattt atatttaaaa
     4921 ttaaatgttc caaacgataa ggtgtatata tgggcagatg gaagaagaat gtacagagta
     4981 tttgaaaatc ttttatctaa tatagcaaaa tattctttag aagaaactag agtctatatt
     5041 gatgtatatg actatggtga aaatgtaaag gtaactatga aaaatatatc ttcttatgaa
     5101 ttaaattttg accctagtga gattatggaa agatttaaaa gggctgatga atcaagaaat
     5161 actgaaggta gtggattagg acttgctatt gcaagagact tagtagattt acaaggtgga
     5221 aaattctata tagaaataga cggagattta tttaaagcaa atttagagtt tcaaaaatat
     5281 gaagaagaag taactaatat ggaaaaagat ggtatttaag tatacatact tttgattgta
     5341 ggaagctctt attataatat attagaatat ttatagtaga ggatgaagag taaaaacaga
     5401 gtagatttaa gccactctgt ttttttattt aaaatcaata atataacaat ttagattaaa
     5461 attgtacata tgtttataaa ataacttata aaataaaaca ttatctaaat gttacattat
     5521 aattccaaat aaatatatag ataccactcc tgtaataata gaaataccaa tagatttaaa
     5581 cttatatgcg acaagaagta caatcataaa agatataatt ttagggttag ctatagataa
     5641 atctaggcta tctccattaa aaaatacatc tttagcagtt aatgaagtaa gtattgctac
     5701 aggtataaat ttcatgattt ttttcaaaaa atcaggcatc tctttttttg agaaaattaa
     5761 cataggaagc actcttggta gatatgtaac aatgtacatt ccaattataa taaaaaatat
     5821 ataattacta ttcataagag tcctcctttt tacagtaaga ttcttctata aagaaaccaa
     5881 ttaaagatgc taaaattgat gcaactatta ttactaaatt gcttttgaat aatgcatata
     5941 agataaccga aataatgatt gatgatataa ataccacaat ataaatctta ttttttattt
     6001 gagaaactat taaagctata aacatagaag tcaatataaa gccagaaata gaactgtcta
     6061 tagatatact ttctcctaaa aatgctccaa taaaattagc taaaactgat gaaattaagc
     6121 agaagaaatt gagataaata gcatttctat cagaccaatt tccagcagta aatttttcat
     6181 aattaactgc aaaagtttcg tctgttatag tatgacaaaa tggcaatata aattttggac
     6241 ttttattttt tatgtacatg ttcaaattag aactcattag aaaatgtctt aagttaacta
     6301 taaatgtagt caaaacaata gatgttacac ttgcaccagt agaaatcatt gaagctgcaa
     6361 tgaattgaga gcttcctcca aatactaaag ttaccattag tgctatttga aaaggtgaaa
     6421 gaccagattt ttgtaatagt attcctccta ctatacctat aggtatataa cctaagaata
     6481 taggaattga agcaataaag ctttctctta actgctgagt tctttccaca ataaatcact
     6541 ccttttgtat taaatgtatt aaagtatttg taaaacataa aaatagatta attttataat
     6601 tgcaattata attaaaaata aatttgaatt aatatataaa ttactgtata acaaatagac
     6661 agtcttaaac tattattaaa ttttatctat ttgttactat tatgtaacaa tacatattaa
     6721 tctctataaa ttaattacta tctgttgtaa ttttatttta acaacaaaaa aaaatacaca
     6781 caattaaaat attgtgtgca atacaattat tttttttctt ctaatagtct ttttaatatg
     6841 ctcagaccag ctttaagctc ttctatactt tctgtagcac atacagctat tcttattgca
     6901 gaaattggtt tttcttttcc aacagcaaaa cgttcagaag cataaacctg tacaccttct
     6961 ttaagtgctt gtatttcaaa tttttctgcc attattccct caggaagaat caaccatcta
     7021 aatatacatt cttcatttcc caaaagatta tacccagata aatattgatt aataatcttg
     7081 tttcttcttc ttgcagattt acgacgtgca ttaattaatt tatcagcttc acctgatgta
     7141 atcatacgat aagcaatttc tgttaaaaaa taagattgag ataaattaat gttgtataat
     7201 gcgtctgata atgattctcg atactgttta ggtgtagaga tatatgctag ccgtaaacta
     7261 ggtgcaataa tttttgataa actcgtaata taaattgttt ggttaggaag ataggaagct
     7321 actggattta aatgtgtttc attaagtaaa ctatgaatag catcctcaat aacaattaaa
     7381 ttgtatttag atgcaatatt tgcaatcatt tttcgtccat tttgtgacat tatatgtgta
     7441 gttggatttt gataatctgg tattatgtat agacctttaa tattttcatt tttacaagca
     7501 tacaatagtc cttcttcact aatttcatta tgttcttgct taattggaat tagttgtact
     7561 ccaagcattt tagctgctgt ctttattcct ggataagtta atgggtctac acctattcta
     7621 tctccatgtt gaaataaacc tgccaaaata gcaacaattg cattttgtcc accacttgca
     7681 ggaagtaagc tatctgctgt agtttcaagc ccagctttat aaattaattt ggcaatagct
     7741 tctttttgcc atttagcgcc atctctttgt ccatattgga atatagtttt aaaatcgatt
     7801 tctttaacta tgtgtttaaa taaagtattc atttcttcta gtgtaaaatc tgggttcatt
     7861 gtacccattt caataaatgt aattttatta ttactggaca ttaagaatag gtttgaacgt
     7921 gtatcataag atacaaaagt tccacttcct gtaactccac ttatgagacc ttttttttca
     7981 caaattttaa aagctctaga gatagtactc acatttacat ctaggaaatc tgccaattca
     8041 cgttggggtg gcaatttagt acctggcaat aaaaacccat tgttgatatc ttgttctagt
     8101 tgttctgcaa gggccttata taatatctga ccttttgaag gtctctttgg cttccagttc
     8161 ataggataat tttcaaaaga attaattggc ataagttttc acctcacgtt attagtttaa
     8221 tttttttctt tatctaaaac aataaattct aaggatacaa ataaaaactg ataggtaata
     8281 attctatcag ttcttttaat tgatttcaaa tcttcctata atttttacac cttctttgtc
     8341 gtaaagagga attgttattt taaatatatt aaaatttctt tttcttgtgt ataaaacagc
     8401 ttcttctggt gtatttggtt gattatcaca atcgtacaaa tctctttttt ttacatagcc
     8461 tactttttta cctctaccta ttgcttgtat aagttctggt tcaattgaat ttccatttga
     8521 gtctattata tcagtaccat aggtttctcc atttttatta taattaattt taccagtagt
     8581 atcttctata tcttcgtttt gtttaaaatt agagttaata gtaaatattg accctattat
     8641 tataaaggtt aacaaaagct taaaacctaa attaattttc tttttatctt ggttaaacat
     8701 ataattcaac ctctttttta aagtaatatt ttttttattt agaaattgag atacaaatga
     8761 tgaatacttc aaagtttttt gatactttat tgtatctaat attatatatg catactcttt
     8821 aatttcacta atgctacatt tgtcaatcac aatttcatca caagataatt cacatagttc
     8881 attaaagtat ttttttaata agtataaaat tggattgaac caatgtatta tacatattat
     8941 actaattaga agtttaaata agttatcttt tcttttataa tgaattaatt catgtttaaa
     9001 gataaatctt aactctttat tattgtactt tttttctgga attattattt cacagcaggt
     9061 taaacctata atggcagggg aataaacacc tttaacctta tatattttga gttttttatt
     9121 tatatttagt tcttttactt gttcatctaa gatactagta atatctattt ctaaaatttc
     9181 ttttttaaaa ttttctacgt aattgtaaaa tttaaggttt gttcttaaat agtgaaaaga
     9241 gattaatata attcctaata tccagatgta cataaatata tctaaaataa tatcctgact
     9301 atatggtata taattattga tattatgttt atagatatta atagtgctat tattgatatt
     9361 taatatgttt aaatttattt ttgatataaa tagcattctg actattatta aaagacatat
     9421 aaaataatta aacttaaaag tatatttgct tatcttttta gtaaataaaa tgaaaaaaag
     9481 aattagtgga cttatgtaaa tattaaattt aataacctgt ataaataatt ccataaaata
     9541 tgtctcctat ttcatttttt ttagccattg ttcaagttct tttatgtcat catctgacat
     9601 ttcattttca caacctaatg atgatataaa gctgtgaata gaatttttat gtataagctc
     9661 taaaaaatcc ttcgtttcaa attttaaata ttcctcatgt ttaactttaa tagtatatat
     9721 agtatttcta ccatcttttt caaattctaa aaaaccttta tcacttaatc tagaaagtac
     9781 tttaccagta gtgccttttg tccatccatg agaattttcc atgtaattag caacttctct
     9841 agctggtatt ttatctatgc ctttatccca tatgaaattc ataactaaca actcagattt
     9901 gggtattttt ttcaatataa tcactccctt taacttgatt atattagaca aatgtctaac
     9961 agtcaatata atatatcatc atttataaag aatatctata ttattttata cttaagaact
    10021 aaatattata tatttataga taaaaaagat attaagagtt ttatggaata gaataatcta
    10081 cttcctacta gatataagaa tataacatat attttttagt gaataaatta atatctgttc
    10141 taagttagaa taggataatc ttattagata aagattatcc tatttattgg aagtttaaga
    10201 tgtattggag attattaatt tttttctttt tttaactaaa agcaataata cataaaatta
    10261 ttattactaa tgaaactcct aatgtggtat atagtcatgg tgttatatga ctatcaatag
    10321 ttttttaagt aattattgta acaaatagca gtatcccaaa aatacttaaa aatatgtttc
    10381 tagacagttt ttaaatataa tataatgtaa cttatatcta taaattaaca ctattatttt
    10441 ccaactccat ttctaggttt tttataaatt tattaaattc ttctggttgc ctttttccaa
    10501 acaatattct tacaagaact gaattgattt ttccaaatac atttctctcg ctcatatttt
    10561 cagtaaaaat aagttttgta ttttctaaat caatatgtat caattcatac ctagtaataa
    10621 tactttctct tttattgctt gtttttatct catatactct atttttttcg aaattagtta
    10681 tttcaacctt aaatctttta ttattttttt gttgtacaaa tgtgcccact gcttttttct
    10741 cattaaaatt aggaaaattt agtcttgcag attttgtaaa tatattaaat atgtcttttg
    10801 cagaatattt taataaaact tcaattttaa atgtattcat atcgtttttc ctcctgtata
    10861 taaatttaat tttataagtt aatttttaag ttaaaatagt ataatatgca atataaaatc
    10921 atgttaaaac tatttatata gctaaaatct tatatagata agtatgtata ctattatata
    10981 aaaataaaat tttaaaacag aaagagttaa aaacatagtt tattgtaatt tttattgttg
    11041 agtagatacg tactattata tatgattgag taagtttttc aataatatat ttataaagta
    11101 ttttgcattg caatttaaac aaagaattaa actatttaag attatgaatg actaatacat
    11161 ttagtaaatt actaaatata caaaatgtaa ttgcttatca aaatgtataa atacttatgg
    11221 catctagtta cttacagggt ttgagtagaa aaaaatatca aagtaagtat ggcatgatag
    11281 ttgcttatat atttaacaag aaaggagtta ttaagcatgg agttgagaga taaacttgta
    11341 aacatcataa atacagaaaa taaaaaagat ccattaacag atgttcaaat agcaaagttt
    11401 ttaagtacaa ctagagagaa tataactaat ttgagaaaag aacttaatat aagtaattca
    11461 aggcaaagaa gatatcctta tctaaaatca gcaatttcga caatattaca aaagaataag
    11521 aatatttcta tatcagaaat tacaagagaa ttgatgacag aaggttttaa tatatctaga
    11581 agagttgtag aggaactttt acctaaagaa agtttagaaa tggaggtagt agaggaaact
    11641 gaagaagaat cgaatgaccc atttgaaact ctgataggta ataaagggag tcttagaaat
    11701 gctgtagaac aggctaaatc tgctatattg tacccaccta aagggttacc gacgttgatt
    11761 gttggagaaa gtggagttgg caagagttta ttttctagac atatgtatga gtttgcaaag
    11821 caaaaaaaga taataaaaga aagttccaat tttgttgtat ttaattgtgc tgactatagt
    11881 gataatccac aattattatt gtctttatta tttggatata aaaaaggtgc atttacagga
    11941 gctgaatatg atgcaccagg tcttgtagaa gaagctgatg agggagtttt atttttggat
    12001 gaaatacata gattgcctcc aaaaggtcaa gaaatactat tttccatctt agatagggga
    12061 aagtttagga gattaggtga aactaatgct gaaagaaaag taagtattat gttgattggg
    12121 gcaactactg agaatgtaga gactaactta ttattaacat tcagaagaag aataccaatg
    12181 ctaataactt tgccaccatt acatgataga ttactaaaag agaaaataga ccttatatat
    12241 aatatctttc aacaagagtg taatagaata aacgcaaaaa tatttgtaga taaaaatgtg
    12301 attgaaatac ttgcactaaa aaagtttagt ggaaatatag gacaattaca aaatatgatt
    12361 caggttcttt gtgcaagagc ttttatgaag tttataaatt ctaaagatga agatagtgaa
    12421 tcgatagtgg tagtagatat aaatgaagtt ttaaagctta aagattcttt taaagatgct
    12481 gcctttcaag aggttgaata tacagagata agaaaatact tgaaaaatgc tatatttata
    12541 ccatttaact tagaagaagc attagtaaaa ggaaatcttt taagtgataa atatatatta
    12601 ccagaggaca tatacaaaac aattgaaaag aaatattatg acctaaaatc cctagatatt
    12661 gaagatatag atatagagaa tattttatgg acttttattt taaatagatt tgctgactta
    12721 aagtttaatc tcgatagtag tgaagatatt ttttcaatga gtgatttaaa tagttttgta
    12781 aatgatagtt tagtcaaact ggtaaaagaa cttatgaatg atatagttga aaaaaatatt
    12841 aatttagaag taaataaaaa tatgttcaaa tatctagcaa tacacttaga agaagctata
    12901 aaaagaatta agctaaatca aaagataata aacgtaaatt tagaaaaaat aaagattgat
    12961 ttttcaaaag aatatgaaat tagtaaaagt tttgcaataa aaattgagga atcaataggt
    13021 attaagattc cagatgatga aattggattt ataacgctct acataaagtc tgcactaaaa
    13081 aatgaagcga aaaaagacaa agtggggtta ataataattt ctcatggacg tatagctaca
    13141 gagactgtaa atgtagttaa agaactttta ggtgtaaagt ttcctgtggc tatagatatg
    13201 cctttagatg aaaaaccaat aaatatatac aataaggcag tagaattgtc aaaaataata
    13261 gaccaaggaa aaggaatatt gtttttggta gatattggct cacttacaaa catagggcaa
    13321 atagtaaata agagaaccaa tataaatact aaaactattg atagagtaga cctcttaatg
    13381 gcactagaag cagctagaaa agtttctatg ggagaggaag aacttgatga aatatttttt
    13441 tctttactaa aagataggat gggatataac tatgaattag aaaagcacat agataaatca
    13501 aatgcaattg taacattatg tcttactgga gaaggaactg ctaaatatat aagtaaaaca
    13561 ttggaagaaa agtatgataa cactaaatgc tatcaaatga gtgcattgga tgaaaatcta
    13621 ttttgtaaaa ttgaaaatat aaaggagaca agcaatatat tggctattgt tgggacaata
    13681 aatcctaagg taccaggtat aaattttata ccctacaata aagaagtttt caaaaatttg
    13741 gatatatatc tatcaaaatc aaaacaacaa gatgacagac caattaaatc gtatgaaaga
    13801 atgcttgatg aggatttagt gatttttgaa ccagatatat attttaaaaa ggatttatta
    13861 gaatatgtat gttcaattct tataaataaa gaatatgtag aaaaagatta tttagattct
    13921 gttttacata gagaggagat gcttccaaca tattctaaag gatttatagg tgtgcctcat
    13981 ggagattctt ctactgtaaa tagtaccagg tttgtttttg tcaaactaaa gaaccctatt
    14041 gattggggtg taggcaatgt aaatttcata tttatgccag tttttcaggc taatgataaa
    14101 gagattgtta agaatgttct tgagatacta aaagatgaag aatttatgaa caatgccaat
    14161 aagtgcttta ataaagatgc ttttaaaaag attatatttg ataaatttaa acatctgtag
    14221 ccatagcaaa aaaattgaga ggagtcagag taatgaatgt agatttaata tcattaggtt
    14281 tagaatctaa tgattatgaa agtgttattg aagaattagg aagtataatg tgtaaaaaag
    14341 aatatgtaaa agacacatac ataaatgctg tcttggaaag agaaaagaca ttaccaacag
    14401 gtctcgatat tggagaaatg tgtgttgcta ttccacatac agattcaaag catgtaaatg
    14461 aatctaatgt agctgtaggg atattgaaaa atcctgtaaa atttaattca atgatagacc
    14521 caaaagatag gcttaatgta gaacttgtat ttttattggc agtaaagaac ccagattcac
    14581 aagtaaaact gttaaaagat ttaatgtcag tttttcaaaa tgtaaagtta ttaaaaaata
    14641 taaaaaacgc atcaacaaag gaagaagtag ctaaattatt ggactttata gaaatataat
    14701 gtatgagaat tttataaata aaaaattaaa atttaaacaa tcaaagtaag tgttattttt
    14761 taataggttt aatatatata aaataaaaat ttagagtgaa aaattaggag gaaattatta
    14821 tgaaacaatt taatgtatta tcagtttgtg gttcagggac agttacatca tctatggtag
    14881 caggtaaatt aaaagaagta ttagggaata agggaatttc tattactaca acagaagcta
    14941 gaccaacaga agcactaaat ttagcgcaat caggaagatt tgattttata acatttacta
    15001 gtccacttca aacaggcgat tatggaatac caactataaa tgcatttgct tgcctaactg
    15061 gtatagggga agaggcattc tttgaagaag ttttgactgt tattgaaggg atagataagt
    15121 aagttttgga gatgtgaatt tatagtattt aaatcaatga tttagatact ataataagct
    15181 aatattaact aaatatttgt aaaaaaacat tttatatgtt gtcataaatt aaaaaattat
    15241 aaaaatagtc atttagatat taaactaagc ttataaatat ttataaatag gaggaaaaag
    15301 atgttagaat ttatgaaatc tataatcgat acatttggtt ctgcaattat agttccaatt
    15361 ataatattca taatagcaaa gatatttaaa gtaactacta agaaatcgtt tctttctgct
    15421 gtgtacgcag gagttgcttt acaaggtttt acattgattt taaattcatt tacaccaata
    15481 ataacaccag ttatcaacag gatggttgag agtacaggag taaatttacc agtttttgat
    15541 gttggctggc aagcaacatc attggttgca ttttcaacaa gtgcaggtat gatatatttg
    15601 ggtcttggga tattattaca aacaatttta tttttgataa aatggacaga cgtgtttcaa
    15661 ccatcggatt tatggaataa ctattcttac atggtatggg gagcaatggt tataggagta
    15721 acaggaaatt ttcctttagg tatagcatgt atggttttat taaacttata tagtttgtta
    15781 atatcagagt tagttgcaaa gagatggtct agttactata gatatcctaa ttgtacgata
    15841 atagctatgc ataatgttga ggcttctgtg tttgcagtat ttgcagaccc tatatataat
    15901 aaattgggat taaataaaat aaaattaaat ccaaaagaat tagagaagaa attaggattc
    15961 ttaggtgaac caattacttt aggtcttttt ttaggtatgt ttataggtat attaggtaat
    16021 atgacaagaa taactactat ggaagcttgg ggagaaataa tgaaagtagg tatatctaca
    16081 agtgcagtta tggcaatatt cccaaaagta gcaagcatgt ttgctcaagc atttgcacct
    16141 ataacagaag cagctagaaa gattatgcaa aaagctggaa accgtgagtg gtacatagca
    16201 gtaaatgatg ctgttgggta tggtgaacct gcaactttaa tatcaggtct tatattaatt
    16261 ccaataatgc ttgtaatagc tatggtactt cctggcaata aagtattacc agttgtggat
    16321 ttattagcaa ttccatatat ggttcaaggt ttagttgcca tacataatgg taatatacca
    16381 aaagtactag tttcaggtat aatttggttt ggattaggat tatatgtttg tacatctaca
    16441 gcaccattat ttacagatat ggctacaaat ataggtgtag ctatacctgc aggagcaatg
    16501 ttaataacaa gttttaatat actaggaaaa cctttgatgg gattagtttt ctttgcattt
    16561 ctaagtgcaa atccaattta tataggtatt gcagttggaa tttactttgc tttatgggca
    16621 ttatttagaa agaacaaagc aagtattctt gattatctag aaaaacaagc acttaaaaat
    16681 gtagaagaag aacctgtagc agtttaagtt tataaaaatg aaataggaat aattaaagga
    16741 gtttataatt gattttatat aagtatcaga tagtacctga gaaatattta aatgaagtat
    16801 agacttcttt ttatttaggt aatatttgta attgtaaagc attgtatttt tatagttgag
    16861 ttttctgtat aacctgtcat ttaaaacata ctcttttata tttatacagt aatgtaattt
    16921 gattttataa aatgtaattc ctattggcaa aagactttgt atataaggta tttacctgaa
    16981 taaaccattt atcttttgta tggcatagat attgctatat agaaatatat agcatgaaag
    17041 gagatattta tatgggaaat aaaatgagag catcagtatt gtataatgta ggtgatgtta
    17101 gatatgaaat ggtagatata ccagaaataa cagatacaca agtgcttgta aatgtaaaat
    17161 atgttggaat atgtggttca gatttaccta ggtctatggt aagtggtttg agtggaaata
    17221 caaaatatcc attgatttta ggacatgaat tttctggtga agttgtcaaa attggagaaa
    17281 aagtaaagca tattaatgta ggagatagag tagccgttgc accattagta ccttgtggag
    17341 aatgtgatta ttgtaatgaa ggtaattttg gcttgtgtga tgattacaat ataattggaa
    17401 caagagtaaa tggagcattt gcagagtatg taagagttcc agaaaaacat gtcttgaaac
    17461 taccagatac actggattat gaaactgctg ctggaattga accagcaact atagcttatc
    17521 atgggatttc aaagtctaat attaaagctg gagatagtgt ggttgtatta ggatgtggtc
    17581 caataggtca gttttctata caatgggcaa gggtatttgg tgcatctaag gtcatagcag
    17641 ttgatatttt tgatgaaaag ttagaacttt ctaaatttct aggagcaaac tatatactga
    17701 actcaaaaga agtaaatgta attaaagaaa taaaaacaat aacaaatggt ggtgctgatg
    17761 ttgtaataga gactgctggt agccgattta cacaagaaca atctctattt gtagcaaaaa
    17821 agagaggaaa tatagtattt gtgggtatat cacacacaga attaccactt agtgcagacg
    17881 ctactgagcg tatattacgt ggtgaactta ctttgaaagg ctcttggaat tcatatacaa
    17941 gcccttatcc tggaagagca tggactgcaa cattggattt tatgggaaaa ggagatatta
    18001 tttttaaacc tatgatttca cataaaatag gtcttaatga ggttggtgac tttttaagta
    18061 aaatgagcaa gagagagatt aatttcaata aaatcctagt agaaatataa aaatttaata
    18121 aaaattctaa aacaattaaa tttcataatt atgacattaa acaaaataga aataattttt
    18181 taaaataaaa tataggagga agaaaagtgt tagaaaattt aaaagaagaa gtattaaaag
    18241 caaatttaga actacctaaa aaaaagctaa ttacttatac ttggggaaat gtaagtggca
    18301 tagatagaga aaaaggattg gtagtgataa aaccaagtgg tgtagagtat agtaatatga
    18361 ctgtagaaga tatggttgta gttgatttag agggaaatgt agttgatggc aaactaagac
    18421 cttcttcaga tactccaact catctagttt tatatagaga gtttgaagaa ttgggaggaa
    18481 tagtacatac gcattcaagt tgggcaacaa tttgggctca aataggaaaa cctataccat
    18541 cttgtggtac tactcatgca gattattttt atggaagtat accatgtacc agaaaaatga
    18601 ctagagaaga aatttgcaat gaatatgaaa aggaaactgg aaatgtaatt attgaagaat
    18661 ttatgggaag aaatccaatt cattgtccag gaataattgt aaatgaccac ggacctttta
    18721 catggggaaa agatgcaaat gaggctgtac acaatgcagt agttttagaa gaagtcgcaa
    18781 agatggctta ttatacagag ctaatgagtc ctgataatct tatggataag gttcttatga
    18841 ataagcattt ttcaagaaaa catggaaaaa atgcatatta tggtcaaaaa tgattgtctg
    18901 gaggtaagtt atgctagagt ctttaaaagg taaagttgtg gaaatagcaa ttaaagcaga
    18961 gcattatggt ttatgtaaag aaaagactgg taattttagt attagagata agaatactgg
    19021 atatatactt ataactccat caggtatagg aagagaagaa ttaaaagttg aacatatttg
    19081 tgtattggat ttggatggaa atgtaataga atttgagaaa ggtattaagc caagcagtga
    19141 ggtattgatg catattgaaa tatataaaac aagggaaaat gtaaatgcta tatcgcatac
    19201 tcattcttta tatgcaacag cattttcagt ggcaaagaaa ccaattatac caattgtata
    19261 tgaatcagtt aattatggcg gttatgtaga tattgctcca tacgaaagac caggaacttt
    19321 aaaattagct aagatcgttt caaaattact gttagataca gatgcatgct tattagaaag
    19381 acatgggttg acaactgtag gtaatgattt agccgaagca ttattaaaat caagatatgt
    19441 ggaagatgta gcagaaatat actataagtc tttagtatta aatcaattta aagaacttga
    19501 agtagtagat gttgatgagt ttaaatcttg gaaataccca gaagaaataa agttttaatc
    19561 taagtaattt ttattgaagt ggttaattaa aatatatgta ttatttatat caattactat
    19621 attgtattat gttaattatc gtcttttgta aatatattta ttacattttt tcaaaaaaat
    19681 atagaatccg aaagccatat aaaattgact tgggattcta tattttttat ttatgcattt
    19741 ttctttatct gattttctgg tgaatttata tgttcaaaat attttttagt ttcagatact
    19801 attataggtg ataatgcaag taaagctatc aagttaggta ttgccataag tccatttacg
    19861 atatcagcaa ttatccaaac aacttctaac tttaagaatg cacctaataa caccatagcc
    19921 acaaatacag ttctataccc attcatacct tttacaccaa acagaaattc aaaacatctt
    19981 tctccataat aactccatcc taaaattgta gtaaatgcaa ataatgttaa actaactgta
    20041 agtaatattg gtccaaatgt aggaatagcc ccgttaaaag ctgcttgtgt cataacagct
    20101 ccatttaggt ctgaacacca tacccctgaa ataacaagag agaatccagt aagagtacag
    20161 attataatag tatctataaa agtacctgtc atagatataa gaccttgttc cgctggccat
    20221 tcagttttag cagcagctgc agctattgga gcactaccta gaccagactc atttgaaaat
    20281 acaccacgag cgataccatt tctaattgct aaggacatag ttgctcctag aaaaccacca
    20341 gtagcagcag taggtgtaaa tgcactttta attattaaca taaaagtttc agggatttta
    20401 ttccaaaaag atactaaaat gattccacag attacaacgt atataactgc cataaaaggt
    20461 actattttag tagctacgtt agatatactt ttaagacctc caaatacaac caaagctaca
    20521 aatactgcta atacaacccc tgtaattcta gggtctatac caaaactatt atttattgcg
    20581 tctgttattg aatttacttg agtaaaagta cctattccta aaagtgcaac tagtatacca
    20641 cttatagcga aaaatattgc aagaggtctc catttttctc ccataccatt tactatgtaa
    20701 tacattggtc caccagacac ttgaccattt ttatctttag ttctgtattt tattgcaaga
    20761 acaccttctg aatacttagt agccattcca aagaatgctg ctatccacat ccaaaaaagt
    20821 gcaccaggtc caccagcttt gatagctgta gcaacaccaa caatattacc agtaccaact
    20881 gtagcagcta aagcagtaca taaggctgca aaacttgaaa catcaccact acctttgttt
    20941 tcagcagaga atattaactt aagagctctt ggtagtttag ttatttgaac aactccaagt
    21001 ttaacagtaa gtagtatacc agtaccaact aatagtacta gtaggggtgg tccccaaata
    21061 aaagcatcaa ctttgtttaa aatatcaatt agattcataa aaacactccc tcattttttt
    21121 tgctttacat aatcaataga atttttaatg aagtaattga caggaaataa gtataattta
    21181 ttaataattt ctagtgtaat aaaaaaagct aaatctaaaa aagattcagc tctggatgta
    21241 cactaattag caaccaaaat aaaagtatta gtaaatactg tttttggtag ataatccctg
    21301 tccttttgcc tgagagattg agcataaaag ccttgcccct tcggcgccca taatgggtct
    21361 ctccagagtt ccgtaaattt tacagtccta ctttaaaagt acctgagagt gttactcctt
    21421 cggtgtgcca tatagcaact cttctataaa atcttaaccg gatattgatt tgtcatagct
    21481 tattgtatta ataatattaa tataagtttt tatgttttgt caactgaaaa attaaaaaac
    21541 aaagaaaaaa gataaaaatg taagcctatt tacgaattat aaacttagaa agatgagtaa
    21601 tatacgtgaa aataaatttt gtaatagaca tacaaattag gtgtaaaata agttttaaat
    21661 aaaaaaacaa taaaatctta aataattaat tagatatgat taaattatgg taatattata
    21721 gttacgatat taagaaaagt tctatttact tagattttaa gcgtaatatg agattatgta
    21781 tagagaaagg atgtattttt agatgggaaa agtattagta ctagcagaaa aaccttctgt
    21841 tggaagagat atagcaaaag ttttagggtc aaaaaatgaa aaaaatggtt atatagaggg
    21901 accaaagtat gtggtaactt gggcactagg tcatttggtt acacttgctg accccgaaag
    21961 ctatggagaa agatacaaat cgtggagttt agaagacttg ccaatacttc ctaagcacct
    22021 taagactgtt gtgataaaaa agagtggaaa gcagtttaat acagtcaaat cgcaaatgaa
    22081 tagaaatgat atagatgaaa tagttatagc aacagatgca ggtcgtgaag gagaacttgt
    22141 agcaagatgg ataatagaaa aatcacaggt aaaaaaacct ataaagcgtc tttggatttc
    22201 ttcaagtaca gataaagcaa taaaagaagg ctttgcaaaa ttaaaaagtg gaaaagaata
    22261 tgaaaatttg tattattcgg caatagcgcg agcagaagct gattggatta ttggaataaa
    22321 tgcaaccaga gctttgacaa caaagtataa tgcacaactt tcttgtggaa gagtacaaac
    22381 tcctactctt gcaatgttac ttaaaagaga agaggaaata agaaatttca aacccaagga
    22441 gtactatgga ttggaattaa ttgctacaaa aggcaattca gatataaaat ttatttggaa
    22501 tgataaaaac aataactctt caacttttag taaagaaaag atagaatcta cattaaaaaa
    22561 agtaaaaggt gtagacataa aaatatctga agtaaacaaa tcatataaga aaaaatattc
    22621 acctgcactc tatgatttaa cagaattaca aagagatgca aataaaatgt ttggattttc
    22681 agcaaaagaa actttatcta taatgcaaaa gctatatgag catcataaag tgcttactta
    22741 tcctagaact gattctagat atttaacatc agatattgtt ggaacattaa aagatagaat
    22801 aaaagctgta aatattagtg attattcaag gatttgtaat aaattattaa aaagcaatat
    22861 tacagctaat aaatcatttg ttgacaattc aaaggtaaca gatcaccatg caataatacc
    22921 aacagaacag aaggtattta tgagtgattt tagtgacaaa gagcgaaaaa tatataatct
    22981 tgtagtaaaa agatttttag cagtactatc tcctcctttt gaatatgagc agacaactat
    23041 aagtgcaact gtagaaggag agatatttaa ggctaaagga aacaaagtaa taaatttagg
    23101 ttggaaagaa agttataaag atattgaaga cgaagatgag tatgaaagta taccaaatgt
    23161 tttagaaaaa gatgtactta aggttaaggg attaaaggta acgtcaggaa aaacaaatcc
    23221 accaccatat ttaaatgagg gaacattact gactgctatg gaaaaaaacg gtctagggac
    23281 agttgctaca agagcagata taatagaaaa attattcaat tcatttttaa tggaaaagaa
    23341 aaataaagat atatatataa catcaaaagg gagacaatta ttagatttag ttccaataga
    23401 cttgaagaca ccagaactta cagcttcatg ggaaaagaaa ttattggata tttcaaaggg
    23461 aaaacttagt aaaaatacat tcataaatga tatgaaagat tattcaaaag atattgtatt
    23521 agaagtaaaa aatagtgata ataaatttaa acatgataac ttgactaaaa atagatgtcc
    23581 agagtgtggt aaatttatgc tagaggtaaa tggtaaaaaa ggtaagatgt tgatttgtga
    23641 agacagagag tgtaatacta gaaaaactat atcacaagta accaattcaa gatgccctaa
    23701 ttgtcataaa aaattagaac ttcgtggaga aggagaaggg agaatcttca cttgtagttg
    23761 cggatacaga gaaaagctat cttcttttaa caagagaaag caagaagaaa agcagaaggc
    23821 atcaaaaaaa gatgttaatc aatatttaaa aaatcaaaag aaaaatcaag aaacatttaa
    23881 taatccattt gcagaagcat tagctaagct aaaaaacaaa taattttaaa gttattatac
    23941 acaattataa ctttaactgt atattagtta tatgtactga tataatatag tgtatagttt
    24001 aggcatgaaa gtagggctgt taaatggatt taagtagtat atttaaacac tattgtaaag
    24061 agtgtgaaaa tacatggaat aattcaagtg ttgaactatt tgataatata gaaacatata
    24121 gtaaagattc acaaaaaaag atggaaaaag aattagataa attgataaat acaatatcag
    24181 ttcatttaga gaggtatcca agtgatattg tattgagaaa aatgtgggta aaaaaaggcg
    24241 agattttctt acaaaagaca ttagaaaaag aaaatatttt taagttagaa aaaatggatg
    24301 tagaggatag aaaaaaattt ttagatataa caaaacaatt tattagagat gctagaaaat
    24361 ttgatagtga tttacctata agtgatatta tgcaagctat gaggaatgta tggatttcaa
    24421 atgcattaca agtattattt ggtaaagaag tttattattc aaaagctaac tttgcatata
    24481 gtatgttata tccatatact gataattatt tagacaatac aagtatagat aaaaatgata
    24541 agattttatt taataactgg ttagaaaaaa ggctcttggg agaacacatt aaatctaatg
    24601 actatcatga gagcaaagta tctcaaatga tagattatat tgaaagtgta taccctagag
    24661 aaaagtttat agaagtatat gaatcattat tattaatatt taaaagtcaa gtaaagagtt
    24721 taaaacaaca tggcgaagaa aatcatttgt gtaaggaaga tttattatcc atttctattg
    24781 aaaaaggtgg ttcatccgtt ttagtagatg gatatttaat aagtggattg atgacaaagg
    24841 aagaaataga tttttgtatc ggctatggat ttttattaca aatatctgat gatttacaag
    24901 atgtaaaaga agatttaaaa tacaaccata aaactattat tacagagatg gcaaaagagg
    24961 gtaatttaga taaagttgta aataaactaa taaattttac tattgagtta atagatagtt
    25021 ttaaaattaa caataaaaat aaatctgtaa taactatgat aaagaatgat tgcttaatgt
    25081 taattttatt ttctgtagtt tataatgctg aatttttttc tgtaggatat ataaaagaag
    25141 tagagagatt tattccatat acaatagact attcattaga gattgaagaa aaaatgaaag
    25201 aaaaatttaa aagtatagat gttttaaata atgaaaacga gtataaagaa atgattgata
    25261 ttatttgtgc agagtagaaa aaaagggagt gtcttagaat agacttttta gtttataagg
    25321 cactcttttt gtataaaatc aaatgtattt tcatcattct aacaattaaa aagaggctta
    25381 tctctatttg agacagccct ttttaattta aataaaaatg tcaaggttaa actatttaaa
    25441 tgcagtaata atcccattat catgtaagtt ttttatgatt gtagctgcga tagggaatcc
    25501 aaataatcca gctattccaa atagttgagc tccaacaaac atacacatta atgttattaa
    25561 aggatatagg ccaatttggt gtcccactac ctttggttct aatatttgtc taacaacagt
    25621 tataattatg tatagtatta aaagtcctat tgctaaatta atatttccat taatgagtga
    25681 tataatcatc caaggtgtca gaatacttcc tgtacccaag actggcaaaa tatcaacaag
    25741 tgctatgatg acagatatag taaatgcatt ttcaactttt aggatagtaa gtccaataag
    25801 tagttctatg aatgtcactg atagtagtat tgcgtaagct tttagtagtt ttaaaagagc
    25861 attaatactg ctatttttta tatttattat cataaattga cttttttctg gaaattgttt
    25921 taagataaag tttactattt tttgatagtc aaaagtaaag aaaaaagaag caattaaagt
    25981 aataataagt tttaacataa atgcaggtgt tttacttgca atactagcaa tagcattaag
    26041 tgcatttgtt gaaatagaag ctactaaaga catcatagat tgacttatat tatcccaacc
    26101 aagcattaat gctaggttaa cattaggtgt actagcatct atcttcgaaa ataaagtatt
    26161 tagtgttggt tgtatacttg atttatatat ttcgggaagt ctaaaaaata catcacttac
    26221 acttgcaaat atcttcacac caaagctaat taataagaat atacacaacc cataaaaagc
    26281 taacagtatt actatagaaa taagtgtacg atttaaattt gtattatttg taataagttt
    26341 tataataggt cttaggatgg atgcaatgat aaaacttatt acaaaaggca ttagtaatgg
    26401 caataagaat tttaatacaa tatatacaat acttgttatt aaaactacat atgtaaattt
    26461 aattataaaa ttcttttgct tttgaatgtc cataatataa aacctccagt gtatttaata
    26521 ttattctaaa tatggtcttt tttacataaa atatactact gtatattgat tataaactat
    26581 aaatcttata taaagtaaat aaattatgta aaatttaggt taattttgta taaagatata
    26641 cttttatatt ttacaattgc taaccaaata ctagaaaata ttttaaataa agaacttaaa
    26701 attgtgataa aattaattta
//
LOCUS       DAFNNB010000034        26532 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0034, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000034 DAFNNB010000000
VERSION     DAFNNB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0034"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            109..300
                     /locus_tag="L3M60_001313"
     CDS             109..300
                     /locus_tag="L3M60_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HBN6225995.1"
                     /translation="MKKVALILAIIGALNWGAIGILGTDLIGSIFGGTYEMISRIIYF
                     IVGLAGLYLIPSLMSDDRE"
     gene            455..1864
                     /locus_tag="L3M60_001314"
     CDS             455..1864
                     /locus_tag="L3M60_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6225996.1"
                     /translation="MFIINELNKIVNEEVISFHMPGHKRGEVYKKLGYKDILENLYKM
                     DTTEILGTDNLHFPEGIIKNSQEKASKVFKSDYTYYLVNGSSCGIQSAIMSVCSPKSK
                     IIANRDCHQSVINGCILGDIDIEYVPCEISEDTNVLKGISISNVIDIIDKNLDAKAVL
                     LTYPTYYGMTYDLEYICNYAHSKNMVVIVDEAHGAHLGLSERLPKTALEQGADIVVQS
                     THKTLPSFTQSSMIHIQGNRVNIDKILNMLRMTESSSPSYLLLSSLELAVDIYENKGK
                     GLMEELLNNIRTFKNNIDSNIKMYDTNDKTKIFISSKNMGLTGYELENILRNKYNIQV
                     ELSNYYGVLLICTIGNTTKDFSSLETALNNVVKEEFKFTKLENKKYPVVIPKKILTPR
                     EAFYKTKKSVKIYDSIGKICGECIVPYPPGISVISPGEVISKEIIDYLMFCHSKGMIV
                     SGLKDVYLNFIQVIDLEYI"
     gene            1985..2674
                     /locus_tag="L3M60_001315"
     CDS             1985..2674
                     /locus_tag="L3M60_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBN6225997.1"
                     /translation="MKGKLIIIESGSDASGKATQTKILYERLKKEGYMIKKVEYPNYE
                     SPSSALVKMYLKGDFGKNASDVDPYIASTFFTADRYASFKTDWEEFYNEGGIVIADRY
                     TTSNMVHQASKMDVCDREKYLNWLADYEFNLFKIPQPDCVVFLDVPIEFSKKLMENRK
                     NKITGEEKKDIHESDIEYLQKSYNNALYIADKYDWKKINCVLDNKLRSIESIHNEIYK
                     IVLDSIKSMER"
     gene            2677..3612
                     /locus_tag="L3M60_001316"
     CDS             2677..3612
                     /locus_tag="L3M60_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBN6225998.1"
                     /translation="MYFENIIGQDFAKKYLTNSIKKNKLNNAYMFEGMDGIGKKKFAD
                     ELSKLLLDYENLENSPDYVLIKPDGNSIKIAQIRNLQSDIVIRPHRDYKIYIINNAEK
                     MTLEAQNALLKTLEEPPNYAIIILVTNNKESLLETIKSRCDIIKFSPIPIEDLKSYLI
                     DTGIEEERAQLLATFSRGSIENALNLSQSSEFSVMREDVQQYIQIMLDKNIIEILNIP
                     NNMEKYRGKIITLLDMMINYFRDIILLKENVNKNMLINVDKLVFIQNMSGKISYSQLS
                     KIIDIIEDVKNKIKSNCNFNISIQVMSLNIYEVIK"
     gene            3609..4502
                     /locus_tag="L3M60_001317"
     CDS             3609..4502
                     /locus_tag="L3M60_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HBN6225999.1"
                     /translation="MIKIVGIRFKSAGKIYYFDPVDFNIEQDMDVVVETARGLEYGKV
                     VVGPKDMDESELVSPLKPIIRIATEEDKKVFLENKEKAKETFELCQQKIKEHELTMFL
                     IDCEYTFDRNKLIFYFTAEGRIDFRELVKDLAAIFKTRIELRQIGVRDEAKSIGGLGP
                     CGRKLCCSSWLGDFQPVSIKMAKDQSLSLNPTKISGICGRLFCCLKYEHDVYSEAIEK
                     MPVVGALVQSDDGKGKVVEVNPLLEQIKVEFQDKTVKIYTREDIKILREPKKCDGCGG
                     KCKDNDGLDEATLRELKKLED"
     gene            4580..5326
                     /locus_tag="L3M60_001318"
     CDS             4580..5326
                     /locus_tag="L3M60_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HBN6226000.1"
                     /translation="MQLKDSERIDDLQLKGLKIIQDTNGFCFGIDAVLLANFTKIKKD
                     AKVVDLGTGTGIIPILIAGKSEAKKIIGVEIQEDVYEMATRSVKLNGLEERVEIINED
                     IKYIDKVLDVNGYHVVTSNPPYMHIDGIKNPNDKKAISRHEVRCNLEDVIRAASRLVM
                     SRGKFLMIHRPTRLVDIITLGRKYRLEPKVIQFVHPRPQKAPNLVLVQFVKDGRPELK
                     ILDPLYVYGEDGNYTKELKAIYNNEDIGEL"
     gene            5327..6160
                     /gene="rsmI"
                     /locus_tag="L3M60_001319"
     CDS             5327..6160
                     /gene="rsmI"
                     /locus_tag="L3M60_001319"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBN6226001.1"
                     /translation="MSGKLYICPTPIGNLEDITYRTLRILKEVDLIAAEDTRHSIKLL
                     NHFEISKPLTSYHEHNKDSKGEYLINKLIDGENIALISDAGMPGISDPGEEIIKQAIQ
                     NNIEIEVLPGATAFVTALVGSGMDTHKFVFEGFLDRDKKVRKAQLEELKEESRTIIFY
                     ESPHRLKDTLKDMFKILGNRNISINRELTKKYQEIIRENIETTIKIFEEKEVRGEFVL
                     IVEGFHGEKSKKENYDELTDREYVLKLIESGMNKKDAIKIVCKDRKLKKDVVYKQVLD
                     L"
     gene            6183..6887
                     /locus_tag="L3M60_001320"
     CDS             6183..6887
                     /locus_tag="L3M60_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HBN6226002.1"
                     /translation="MEIGKIIIESLKREDTHKTFIELDDKHELEKIIPKVSCMKSVGE
                     CKYHVVNCFEHSINALKELEIVLNNKDFFPIHLRKHVSNYLNTYIEEGINNLHVLKLG
                     TFLHDIGKPDSMTLDETGRVHFTNHEKIGAQIIDNMGINLKLSTETYRLISKYVRYHM
                     ILLSLYKKNDLSRKELINVFNLVDEDTIGVIMLGYADIVSTVKLLGKSEEVSVLKTYM
                     EYILTNYLYKSNYTHV"
     gene            complement(7075..7503)
                     /locus_tag="L3M60_001321"
     CDS             complement(7075..7503)
                     /locus_tag="L3M60_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HBN6226003.1"
                     /translation="MDVTDYRIIEILQDDGRISMKDLGKIVGLTSPAVSERVKRLEES
                     GVIEGYKAIVNPDSLGRVIKAFIHISLPSNGYTEFIESAAKDPRIVECHHITGDDCLL
                     LKVIVKDMYELENVIDTIKKIGSTKTSVILSTPIQAKSIL"
     gene            complement(7540..8970)
                     /locus_tag="L3M60_001322"
     CDS             complement(7540..8970)
                     /locus_tag="L3M60_001322"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBN6226004.1"
                     /translation="MRNLTLLTDLYQLTMLNGYFEKNIHEDIVVFDMFFRKNACDGGY
                     TIVCGIDQVVEYIDNLHFSDEDLKYLKNLNLFSDKFLKFLKEFKFTGDIYAVEEGTIM
                     FPNEPLITVKAPLYQAQLIETALLTIVNFQSLIATKASRVCFAAQGDPVFEFGLRRAQ
                     GPDAGIYGARAAVVGGCAGTANVLAGKMFDIPIIGTQAHSWVQKFDNELEAFQAYADV
                     YPDKCLLLVDTYDVLNSGVPNAIKVFKNISEKGYKPMGIRLDSGDLAYLSKEAKKQLD
                     NAGFSDISITASNDLDEYTITSLKAEGATINSWGVGTKLITSFDSPSLGGVYKLAASC
                     EKGVLEPKIKISENPEKINNPGYKKVIRIYNEDNKAEADLIMLHDEVIDESKPLEIFH
                     PTYTWKTKVFTNYKVKELLKPLYIKGICKYNKKAVLEIKNHVQYELSTIWEQYKRLSK
                     PHIYKVDLSRNLWYLKTQMIDSKKVL"
     gene            9102..9686
                     /locus_tag="L3M60_001323"
     CDS             9102..9686
                     /locus_tag="L3M60_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBN6226005.1"
                     /translation="MIIIGKIWFYMVSIGIIGSLISKNMGELNKVILSETSKGIEFAI
                     SLAGIMALWMGIMNIAKESGLIEKIGQKLNPIMRRLFPSIPKGHKAMSYIVMNIALNM
                     LGAGNGATAFGLKAMSELQTLNNKKDTATNDMIMFMVINISSIQIIPFTIIKLRMDMG
                     AQNPSEIIFTTLFATIISTVIAIITCKFFQRRYR"
     gene            9702..10223
                     /locus_tag="L3M60_001324"
     CDS             9702..10223
                     /locus_tag="L3M60_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore maturation protein"
                     /protein_id="HBN6226006.1"
                     /translation="MEIFSNLLIPIIILYIVVYGKYKKIDVYDSFVKGAIDGLKAAWD
                     ILPYIIGIFLAIGIFKTGKGLDMLEWLFTPIANMMSIPKELIGLISVKPLSGSGALGM
                     YSELANRVGIGSLVEKMGATIVGSSETIFYTMAIYYGSLKIKNTRHTLSCAMISHVAG
                     VIAAVFICYVIFV"
     misc_binding    10289..10536
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10620..12557
                     /gene="metG"
                     /locus_tag="L3M60_001325"
     CDS             10620..12557
                     /gene="metG"
                     /locus_tag="L3M60_001325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBN6226007.1"
                     /translation="MSKPSFYVTTPIYYPSGGLHIGHTYSTVAADTIARFKRFCGYDV
                     KFLTGTDEHGEKIQKKAIEQGMSEIEYLDGMIKDIKALWNTMDISYDDFIRTTEKRHT
                     DIIQKIFTKLYEQGDIYKGEYEGRYCTPCESFWTESQLLEGNKCPDCGRETYLVKEES
                     YFFRLSKYEDRLKELFKDDSFCFPAARKNEMVANFLDKGLEDLSVTRTTFDWGIKVPF
                     DEKHVIYVWVDALCNYITALGYMTDNDEEFKKYWPANVQIVGKEIVRFHTIIWPALLM
                     ALGLEVPKQVFGHGWILFADDKMSKSKGNVVYPEPIIERYGIDTLKYFLLREFAFGQD
                     GSYTHRNFVTRINYDLANDLGNLISRTVAMVEKYNNGIIPTAKVSTDFDADLKEQAVS
                     TRENFEAEMDKMQFHEALESVWKLVRRTNKYIDETMPWALAKDEAKKDELDTVLYNLC
                     ESIRIIATLINPIMNETANKIYEHIGIKGQDDITTWESTKTFGLIGENVKVFKGEPLF
                     PRLDVEKEIEELTKMFSGKPPVEEKPLEHKEEITIDDLDKIELRVGKILSCEKHPKAN
                     KLLVSQVKIGPETRQIVSGIAEYYKPEDLVGKEVTVVCNLKPVKLRGVESQGMILAAG
                     DDGDPYVLPFTQGAKDGCEVR"
     gene            12587..13357
                     /locus_tag="L3M60_001326"
     CDS             12587..13357
                     /locus_tag="L3M60_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HBN6226008.1"
                     /translation="MLFDSHAHLNDESFDEDRDELIGSLKDKGVDLVVNPGADIDTSI
                     TAIELAKKYDFIYSAVGVHPHEVSKLDDTAIEILRKLATENEKVVAIGEIGLDYYYDY
                     SPREEQKEWFKKQIELANELKLPIIIHDRDAHGDTFEIIKNTKSPEIGCVLHCYSGNV
                     ELAREYVKMGCYISIPGTVTFKNNKKTREVVREIPLERLFIETDSPYMSPEPHRGKRN
                     NPSQVSFVADKIAQEKGISYEEVCRVTKENAKKFFNIK"
     gene            13695..13966
                     /locus_tag="L3M60_001327"
                     /pseudo
     CDS             13695..13966
                     /locus_tag="L3M60_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892105.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
     gene            14128..14964
                     /locus_tag="L3M60_001328"
     CDS             14128..14964
                     /locus_tag="L3M60_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HBN6226009.1"
                     /translation="MLTELHCHTNISDNNFTTEEIIKKAAEKNINILAITNHDTLSGL
                     DEAIVLGEHYGITIIPGIEISAYDYINRKRVHILGYNIDLNSQEIKNLCNPMVCDRHK
                     ASVKMVNKIIDLGYKISLEDVKKYSSKTGIFKQHIMKALIDKGYTDNIYSSLYKELFH
                     RGNGKVYVSLRYVDYRDAIKAIKNSGGICVLAHPGQMDNFSAIEEMVKVGLDGIEVYH
                     PSHNKELEKESLSYAKKYNLVVTGGSDYHGFYSQHNYELGSKSLNKEDLFKFRLALGG
                     VL"
     gene            14965..15960
                     /locus_tag="L3M60_001329"
     CDS             14965..15960
                     /locus_tag="L3M60_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6226010.1"
                     /translation="MSFLQVKNVCKSYGQVKVLKDISIDIEKGEFICLLGPSGCGKST
                     LLRIIAGLEDKHGGKIIINDRDMTNSPPESRNFGIVFQSYALFPNMNVYKNIAFGLEN
                     KNISKSNIDKKVKKVLEVVELSGYEKKYPSQLSGGQQQRVALARAIALEPDFLLLDEP
                     LSALDAKVRLKLREQIRSLHRKLGITTIMVTHDQEEALCLADKMVVMNRGEIIQVGTP
                     KEVYKNPETPFVADFIGTINFIDNGINKIAIRPEDIKVEFNRDSKDKDIKEGEILDIE
                     FRGFNYRITVEYCSKQMKLDVVSKVAEQMKLCIGSKINFKIPKEGIVQYKSEGCA"
     gene            15978..17336
                     /locus_tag="L3M60_001330"
     CDS             15978..17336
                     /locus_tag="L3M60_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBN6226011.1"
                     /translation="MITSKNKRRISDSTIQKYFLIFSVIVLIVFILFPLASLLKNAFM
                     DTSGNFIGILNFSKYIENPSLIASFKNSIFVSIVSSLISLCLAFIYSYAITRTNIKFK
                     NFFKTMGMFPLFAPTMLYGISLIYLFGNKGIFTTMGLEVPLYGPLGIIISQILFTFPQ
                     AFLILSVALSMADYRLYEAADSLGASEFKKFCKITIPGIKYSLLSSFFVSFILAFTDF
                     GAAKVVGGNYNVLATDIYKQVVGQFNIPMGATVSMVMLIPVLIAFTLDKMASKKQGMT
                     ITAKSMPYKIKENKSRDRAFLIVCSLITLLIFILIFVSVIGSVIKLWPYNLSFTMEHY
                     TFKGILGSGIKTYLSSLKISTLTAVFGTIFVFFSAYMIEKVDKFPKLRQIAYFLSMIP
                     MALPGLVLGISYITFFNSASNPLNFLYGSTAILVIVNIVHFYSVCFITSNSSLKMLDI
                     EV"
     gene            17327..17671
                     /locus_tag="L3M60_001331"
                     /pseudo
     CDS             17327..17671
                     /locus_tag="L3M60_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
     gene            17863..18393
                     /gene="rnmV"
                     /locus_tag="L3M60_001332"
     CDS             17863..18393
                     /gene="rnmV"
                     /locus_tag="L3M60_001332"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HBN6226012.1"
                     /translation="MIKEIIVVEGRDDVTAVKRAIDAELITTGGFGFPKGVMERIKAA
                     NERRGVIIFTDPDFAGEKIRKKIASEVPGCKHAFLPREEAKKDGDIGIENATPQSIIA
                     ALNKVRTENTEKRDEFKQIDLIRNGLIGNEEASLRRDALGKILGIGYGNAKQFLNRLN
                     NYGVEREAFIDALKQI"
     gene            18564..19433
                     /gene="rsmA"
                     /locus_tag="L3M60_001333"
     CDS             18564..19433
                     /gene="rsmA"
                     /locus_tag="L3M60_001333"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBN6226013.1"
                     /translation="MDRLSSHNATKEVVQKYNFKFSKSLGQNFLIDSNIIDKILSGAR
                     ITGGDNIIEVGPGIGTLTREMGKIAEKVVAIEIDRNLIPILKDTLSDLENTEVVNQDI
                     LKVDIQELVKDKLNGGPVKLVANLPYYITTPIVMKFLEEDIPVTDIVVMVQKEVADRM
                     NAIPGTKDYGALSIAVQYYCDTEIVAKAPRHMFIPQPNVDSTVIGLHVRDKRKYDVHN
                     EEIFFKTVKASFGQRRKTLLNSLGGLGFLNKDEIREILKEANIDEKRRGETLSIEEFS
                     VLSNIINTKVSSK"
     gene            19499..20761
                     /locus_tag="L3M60_001334"
     CDS             19499..20761
                     /locus_tag="L3M60_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226014.1"
                     /translation="MGSKRKFKRDYIILEAKDINFRYKERVLPKAFAKVEVNDEKAII
                     ALYVENLKYVKDGYKAVAIKDDHGLIDLGNIILNEQGRGEFVLDLEDYDDEIKGIALL
                     YERNIPLIGFKGNKLENYEDILFQEVEEEEYEELEEEDEEVEDNEESNRDEDIEEEEY
                     IIEEYDEDEYEDYWEDDEDIEEGELKARTEVEDSSNEEIEEFNNYKEIENYNRVKLQE
                     EYIDIECEEDEEEDEEEVFEVNREDDRFVSSKPTKSKKVQKEQNNIEKIKRVNGVSQG
                     ESGNKQTSTLLMPRQIKKGLKLFKEVKPFVTDYIDKTRWWKIEINPTTLCGYTMPYLG
                     YLNVLNYTMYSDAVLHSYKYRHYLFGVQYDEYNKRKHYIYAIPGNRNEQPDNGSTGFL
                     RYQACDNRTNSLGYWLCFIDSRQRNIVR"
     gene            20912..22027
                     /locus_tag="L3M60_001335"
     CDS             20912..22027
                     /locus_tag="L3M60_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBN6226015.1"
                     /translation="MINEQRILNEFLELVQIDSLSLKEGNVAKVLVKKLEEIGCSVVV
                     DNAGEKANGETGNIIATLKGNKEGKKILFSSHMDTVTPGIGVKPIVDEVNGIIKSDGT
                     TILGSDDKAGIAAILEGLRYINENNIEHTDIQVVFSICEECGLVGAKNLDYGKIDSEY
                     AFILDSGGSPGEIIVKAPAQDVINVKILGKTAHAGLEPEAGISAIMVAARAIENMNLL
                     RIDEETTANIGIINGGTATNIVTGEVNIVAEARSLKENKLDVQTKHMVETFEKAAKDF
                     GAQIEIDVNRAYTPIDVSEDSEIIKLAKKAFSNLGIEGHTESTGGGSDTNILSKNGIE
                     AITLGIGMKNAHTLSEHIAIKDLYDSAKMVVEIIKEA"
     gene            22213..23109
                     /locus_tag="L3M60_001336"
     CDS             22213..23109
                     /locus_tag="L3M60_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBN6226016.1"
                     /translation="METRYEEANKITIQSILWNVVLTIIKVIAGVIGNSSAMIADGLH
                     SASDIISSIGVLIGNYVSSRPGDREHNYGHEKAETLVSFVLSILLIFVSITIGIEAIK
                     SLFNLDALSVPSILPLVVSVISILIKEYQYRITIKVAKKINSPALKADAWHHRSDALS
                     SVAAFIGIGGSILGFKPLDPIASVVVAIFVAKVGISILISSVNELMDVSVDEEEIKEL
                     KFIVADTEGVKNLGDIKTRKHGAMAYVDLTICVDEDLTVKQGHDIATKLEKHIIKHME
                     FVKGITVHVEPCTNCQGNKCNN"
     gene            23179..23976
                     /locus_tag="L3M60_001337"
     CDS             23179..23976
                     /locus_tag="L3M60_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226017.1"
                     /translation="MSKKRKLKKKAAILIGVVVFFIIYFISFRIGINMEKDKSEVPKK
                     VEKEEEDNRSLRTQIKEKKKVYISDKNIENIKIEEDMWDEIKLFFSDFKKVRNTTSSY
                     EAVYEGYSDDGVKFSTDLNFFRVYTVNKEEYYKVPVSSKDAFKKLLDESIYTSFDLLS
                     HYKDWKEVTITYGKEIKKVHRWKFDDLAHKMISKRVVGKVQPEKSKERSDYNFTINIK
                     GDNFDVKVDTMGKDYVKVTSKKNVSYYEVHTSLFDYLKNEIFEIGKK"
     gene            complement(24031..25383)
                     /locus_tag="L3M60_001338"
     CDS             complement(24031..25383)
                     /locus_tag="L3M60_001338"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBN6226018.1"
                     /translation="MNIHFIGIGGISMSALAEICINKGYQVSGSDSNESYLLDKLRDQ
                     GATIYIGQKKDNISDDVNMVVYTAAVHPDNEELVAAKEKNKLVMNRATFLGQIMREYK
                     NSIAVSGTHGKTSTTSMLSTIFEYADLDPTILVGGNLSMIGGNVKIGNSNHFITEACE
                     YVDSFLNFNPKISIVLNVEEDHLDYFSGIDEIKASFNKFGKLLPPEGYFIINGDDENI
                     DDILYDVKATIIKYGRDSDNDAVIKDIHFDNSGHGIFRIEYQGRDLGEFELSVYGLHN
                     IYNASSAIMAALVSGIDLETIRKNIKIYKGVGRRFETKGYYKNALVVDDYAHHPTELK
                     ATLAAAKKLKKSTLWCIFQPHTYTRTKSLLGEFSEAFYAADKVIITDIYAAREKDPGD
                     IHSKDLVEKLYQNNVDAIYIKEFEDIVKYLRENVKDNDLVITAGAGPIYKVADLLVEK
                     "
     gene            25602..26480
                     /gene="purR"
                     /locus_tag="L3M60_001339"
     CDS             25602..26480
                     /gene="purR"
                     /locus_tag="L3M60_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HBN6226019.1"
                     /translation="MKFKRTERIGAIVKILSDNPNKIYTLSYFTNQFNAAKSTISEDL
                     LVVKNVFEKLHLGKVITISGAAGGVKYIPKTSIAENAEFLMELCERICDKSRILSGGF
                     LYLIDIIYDPTIAAKIGKIFASNIEYVDADYVVTMETKGIPMALMTAKAMNLPLVIIR
                     KDIKVSEGPTLSMTYVSGNSSKVESMSLPRKALKPDSKVIIIDDFMRGGGTIKGMVDL
                     MNEFGAEVIGTGVFISTTNPSEKMVKDYISLIQLDVNGDKIVVEPNLKTFKDEYRNED
                     LDEEEDSEFEFEIDED"
BASE COUNT        10560 a         2916 c         4721 g         8335 t
ORIGIN      
        1 ttttttgtca aaaaaaacaa aaaatacgta tttctttttt catttcggga aaactaaaca
       61 caggaattat ggactttata aaaatttaat tctcgaggag tgatataagt gaaaaaagtt
      121 gctttaattt tagctataat aggtgcgctt aactggggtg caattggtat tctaggcaca
      181 gacttaatag gtagtatctt tggtggaaca tatgaaatga taagtagaat aatatacttc
      241 attgtaggtt tagctggatt atacttaata ccaagtttaa tgtctgatga tagagaataa
      301 tttatgcttt tatgttaaat acctcattct aaatatttag aatgaggtat ttaattttat
      361 aaaaatatta atttgtttct tttttaatgg taaaatagat aagaattgaa gttttaaaaa
      421 gaagtattat ctaaaaaagg agtactatta atatatgttt attattaatg aattaaataa
      481 gattgtaaat gaagaagtaa tatcttttca tatgcctggt cataaaagag gagaggttta
      541 taaaaagttg ggatataaag atatactaga gaatttgtat aaaatggata caacagagat
      601 attaggaaca gataatttac attttccaga agggattata aaaaattcac aagaaaaagc
      661 atcaaaggta tttaaaagtg attatactta ctatctagta aatggtagta gctgtggaat
      721 acaatctgca ataatgtcag tatgcagtcc aaaaagtaaa ataatagcaa atagagattg
      781 tcaccagtct gttataaatg gatgtatttt gggagatata gatatagagt atgtaccttg
      841 tgagataagt gaagatacaa atgttttaaa aggtatatct ataagtaatg tgattgatat
      901 aatagataaa aacttggatg caaaagcggt actgcttact tatcctacat attatgggat
      961 gacatatgat ttagaataca tatgtaatta tgcacatagc aaaaatatgg tagtgatagt
     1021 agatgaagca catggtgcac atttagggct tagtgaaaga cttcctaaaa cggctcttga
     1081 gcaaggtgca gatatagttg tacagagtac acataaaact cttccatcat ttacacaatc
     1141 gtcaatgata catatacagg gaaaccgagt taatatagat aaaatattaa acatgttaag
     1201 aatgacagaa tcttcaagcc cttcatactt attattatct tctctggaat tagcagtaga
     1261 tatatatgaa aataaaggaa aaggattaat ggaagaactt ttaaataata ttagaacttt
     1321 caaaaataat attgattcaa atattaagat gtatgacaca aatgataaaa caaaaatatt
     1381 tatatcatct aaaaatatgg gtttaacagg ttatgaatta gaaaatattt taagaaacaa
     1441 atataacata caagtggaat tatctaatta ttatggagtt cttttaatat gcacaatagg
     1501 aaatactact aaagatttct ctagtttaga aacagcatta aacaatgttg taaaggaaga
     1561 atttaaattt acaaagctag aaaataaaaa ataccctgtt gttattccga agaaaatatt
     1621 aacacctaga gaagcatttt ataaaactaa aaaaagtgtt aaaatatatg acagcatagg
     1681 taaaatttgt ggtgaatgta tagtgccata cccacctgga ataagtgtaa tatctcctgg
     1741 tgaagtaata agtaaagaaa taatagacta cctaatgttt tgccattcta aaggtatgat
     1801 tgtgagtggt ttgaaagatg tatatttaaa ttttatacaa gtaatagatt tagaatacat
     1861 ttaaaactta tgatataaaa gtagatagtt taaaatttcg aagagaaata aatagactgt
     1921 ttatatagag ctatataata agagtattaa aaaagtaaaa agtatttgta tggaggatat
     1981 gaaaatgaaa ggtaagctaa taattataga gagtggttca gatgcaagtg gaaaggctac
     2041 tcagacaaaa atattatatg aaagattaaa aaaagaaggc tatatgatta aaaaggtaga
     2101 atatccaaat tatgaatctc catcatctgc attggtgaaa atgtatttaa aaggagactt
     2161 tggtaaaaat gcatcagatg tagacccata tatagcatca acttttttta cagctgatag
     2221 gtatgcatca tttaaaaccg attgggagga gttttacaat gaaggtggaa tagttatcgc
     2281 agatagatat acaacctcta atatggtaca tcaggcctct aaaatggatg tttgtgatag
     2341 agaaaagtat ttaaattggt tggctgatta tgaatttaat ttatttaaga taccacaacc
     2401 tgattgtgtt gtatttttag acgtgcctat tgaatttagt aaaaagctta tggagaatag
     2461 aaaaaataaa attacagggg aagaaaaaaa ggatattcat gaaagtgaca tagagtattt
     2521 acagaaatct tacaacaatg ctttatatat agcagataaa tatgattgga aaaaaataaa
     2581 ttgtgtttta gataacaaat taagaagtat tgaaagtata cataatgaaa tttataaaat
     2641 agttcttgat tcaataaaat ctatggagag ataaatatgt attttgaaaa tattataggg
     2701 caagattttg caaagaaata tttaacaaat tctataaaga aaaataaact taataatgcg
     2761 tatatgtttg agggaatgga tggaatagga aaaaagaaat ttgctgatga gttatcaaaa
     2821 ctattacttg attatgaaaa tctcgaaaat agtccagact atgtactcat aaagccagat
     2881 ggaaatagca taaaaatagc tcaaatcaga aatttacaat cagatatagt aataagacct
     2941 catagagact ataaaatata tataattaat aatgctgaaa aaatgactct ggaagctcaa
     3001 aatgcacttt taaagacgct ggaggaacct ccaaactatg ctataataat cttagtcaca
     3061 aacaacaagg aatctttatt agaaactata aaatctagat gtgatataat aaagttctca
     3121 cctataccaa ttgaggactt aaaaagttat ttgatagata caggtataga agaagaaaga
     3181 gctcaattat tggctacttt ttcgagagga agtattgaga atgctctaaa tctatctcag
     3241 tcttctgagt tttcagttat gagagaagat gtacaacagt atattcagat aatgttagat
     3301 aaaaacataa tagagattct aaatatacca aataatatgg aaaaatatag aggtaagatt
     3361 atcactttat tagatatgat gataaactat tttcgagata ttattctatt aaaagaaaat
     3421 gtaaacaaga acatgttaat aaatgtagat aagctggtct tcattcaaaa tatgagtgga
     3481 aaaattagtt attctcaatt gtctaagatt attgatataa tagaagatgt aaagaataaa
     3541 ataaaaagca attgcaattt caatataagc atacaagtta tgtctttaaa tatatacgag
     3601 gtgattaaat gataaagata gtaggtataa ggtttaaaag tgctgggaaa atatattatt
     3661 ttgaccctgt tgattttaat atagagcaag atatggatgt agttgtagaa actgcaagag
     3721 gcttggagta tgggaaagta gtcgttggtc caaaagatat ggatgaaagc gaattagttt
     3781 ctcctttgaa gccaattata agaatagcta cagaagaaga taaaaaagta tttctagaaa
     3841 ataaggaaaa ggcaaaagaa acttttgagt tatgtcaaca aaaaataaaa gaacatgagt
     3901 taacaatgtt tttaatagat tgtgagtata catttgatag aaataagttg attttttact
     3961 ttacagcaga aggaagaata gattttagag aattggtaaa agatttagct gctatattta
     4021 aaactagaat agaacttaga caaataggtg tcagagatga agctaaatct ataggaggtc
     4081 taggtccttg tggtagaaaa ctttgttgtt catcttggtt aggtgatttt caaccagttt
     4141 caataaaaat ggcaaaagac caaagtttat ctctaaatcc aacaaaaata tctggaatat
     4201 gtgggagatt attctgttgc ttaaagtatg agcatgatgt atatagtgag gcaatagaaa
     4261 aaatgcctgt tgtaggtgca ttggtgcaat cagatgatgg gaaaggaaaa gttgtagaag
     4321 taaatccttt attagaacaa attaaagtag aatttcaaga taagacagtg aagatatata
     4381 caagagaaga tataaaaata cttagagagc ctaaaaaatg tgatggctgt ggtggtaaat
     4441 gtaaggataa tgatggatta gatgaggcca cactaagaga gctaaagaaa ttagaagatt
     4501 aaattcaagg tagggctttt cctacctttt ttaaagcaat taatgcttaa agttattaat
     4561 atttgaggag aatttatata tgcaattaaa agattcagaa agaatagatg atttacaatt
     4621 aaaaggcctt aaaattatac aagatactaa tggtttttgt tttggtatag atgcagtttt
     4681 attagccaat tttactaaaa taaaaaaaga cgctaaagta gtagatttgg gtactggaac
     4741 tggcataata cctattttga tagctggaaa aagtgaagct aaaaaaatta ttggtgtgga
     4801 aatacaagaa gatgtatatg aaatggcaac tcgttctgta aaattaaatg gattagaaga
     4861 aagagttgag attataaatg aagatataaa atacatagac aaagtcttag acgtaaatgg
     4921 ttaccatgta gtcacatcaa atcctccata tatgcatatt gatggaataa aaaatccaaa
     4981 tgataaaaaa gcaatatcaa gacatgaagt aaggtgtaat ttggaagatg taattagagc
     5041 tgcatctaga ttggttatgt ctagaggaaa atttctcatg atacatagac caacaagatt
     5101 ggttgatata ataactttag ggagaaaata tagattagag ccaaaggtta ttcaatttgt
     5161 gcatccaaga cctcaaaaag cacctaattt agtcttggtt caatttgtaa aagatggaag
     5221 accagagctt aaaatattgg acccattgta tgtttatgga gaagatggga attatacaaa
     5281 agaattaaaa gcaatatata ataatgaaga tataggagag ttgtagatga gtggaaaatt
     5341 gtacatatgt ccaacaccaa taggaaactt agaagatata acatatagaa ctttaagaat
     5401 attaaaagag gtagacttga ttgcggctga agatacaaga catagtataa aacttttaaa
     5461 tcattttgaa atctcaaagc cattaactag ttatcatgaa cataataagg attcaaaggg
     5521 agaatatttg ataaataaac taattgatgg tgaaaatata gctctgataa gtgatgcagg
     5581 tatgccaggt atatctgacc caggagaaga gataataaaa caagctatac aaaataacat
     5641 agagatagaa gtattaccag gtgcaacagc atttgtaaca gctttagttg gttctggtat
     5701 ggacacacat aaatttgttt ttgagggctt tttagataga gataaaaaag ttaggaaagc
     5761 acaattggaa gaattaaagg aagaaagtag aactataatt ttttatgaat caccgcatag
     5821 attgaaggat acactaaaag atatgtttaa aattcttggt aataggaata tttctataaa
     5881 tagagagctt acaaaaaaat atcaagagat aataagagaa aatatagaaa ctacaataaa
     5941 gatatttgaa gaaaaagaag taagaggaga gtttgtatta atagtagagg gttttcatgg
     6001 tgaaaagtca aagaaagaaa attatgatga gcttactgat agggaatatg ttttaaaact
     6061 aattgaaagt ggtatgaaca aaaaagatgc aataaaaata gtttgtaaag atagaaagtt
     6121 aaagaaagat gtggtttata aacaagttct agatttataa aacctgaatc tgaggtgatt
     6181 ttatggaaat aggaaaaatt ataatagaga gtttaaagag agaagataca cataaaacat
     6241 ttatagaatt agatgataag catgaattag aaaaaataat tcctaaagta agctgtatga
     6301 aatctgtagg tgaatgtaaa tatcatgtag taaattgttt tgaacactct ataaatgcat
     6361 taaaagaatt agaaatagta ttaaataata aggatttttt tccaatacat ctaagaaaac
     6421 atgtttcaaa ctatttaaac acatatattg aggaaggaat aaataattta catgtattaa
     6481 agttaggaac atttttacat gatataggta aacctgatag catgacttta gatgaaacag
     6541 gaagagttca tttcacaaat catgaaaaaa taggtgctca aataattgac aatatgggga
     6601 taaatctgaa gttatcaaca gaaacatata gacttataag taaatatgta aggtatcata
     6661 tgatactttt gtctttgtat aagaaaaatg atttaagtag gaaagaatta attaatgtat
     6721 ttaacttagt agatgaggac actataggag taataatgct tggatatgca gatatagtat
     6781 ctacagtaaa attattaggt aagagtgaag aagtcagtgt attaaagaca tatatggaat
     6841 atattttaac aaattacttg tataaaagta attatacaca tgtataaatt tagtcaatat
     6901 aattgagttt taaatggtta attgaagttt gaaatatata gatttcaaaa acataaaaga
     6961 agttgtcttt gttgtaaaat tatgaaagac aacttttttt atatcaaaaa ttgaaatttt
     7021 attaaggata cttaatggtc tattaattaa taaaccatta gtatttaatt tatattacaa
     7081 tattgatttt gcttgtattg gcgttgatag tataacagag gtcttagtag aaccaatttt
     7141 ctttatagta tcaatcacat tttctagctc gtacatatct tttacaatta cttttaaaag
     7201 taagcaatca tcacctgtta tatggtgaca ttctacaatt ctagggtcct ttgcagctga
     7261 ctcaataaat tctgtatatc cattacttgg aagagatata tgaataaatg cctttataac
     7321 tctacctaat gaatctgggt tgacaatagc tttatatcct tctataacac cagactcttc
     7381 taatcttttg actctttctg aaactgcagg agaagttaaa ccaactattt ttcctaagtc
     7441 cttcatagaa attctcccat catcctgtaa aatttctatg attctgtaat ctgtaacatc
     7501 cataaataaa cagtccccct taaataaaaa taattaatgc tataaaactt ttttagaatc
     7561 tatcatttgt gtttttaaat accaaagatt tctagataaa tcaaccttat aaatgtgtgg
     7621 ttttgaaagt cttttgtatt gttcccagat agtacttaat tcatattgta catgattttt
     7681 tatttcaaga acagcttttt tattatattt gcatatgcct tttatataca atggtttaag
     7741 aagctcttta actttgtaat ttgtaaatac ctttgttttc catgtataag taggatggaa
     7801 gatttctaaa ggtttagact catctataac ttcatcatgt aacataatca aatctgcctc
     7861 tgccttattg tcttcgttat atattcttat aactttcttg tacccaggat tatttatttt
     7921 ttctggattc tcagaaatct ttattttagg ttcaagaact cctttttcac aagatgcagc
     7981 taatttataa actcctccaa gagaaggtga atcgaaagat gtaatgagct ttgtacctac
     8041 tccccatgag tttatagtag ctccctctgc tttaagtgat gttatggtat attcgtctag
     8101 gtcattagat gcagtgatgc taatatctga aaagccagca ttatctaact gttttttagc
     8161 ttctttagat aaataagcta aatctccaga gtcaagtctt atacccatag gtttgtatcc
     8221 tttttcagaa atatttttga atacttttat tgcattagga actccactat ttaacacatc
     8281 ataggtatct acaagtagta agcatttgtc tggataaaca tcagcatatg cttggaatgc
     8341 ttcaagttca ttatcaaatt tttgtaccca actatgagct tgtgtaccaa ttataggtat
     8401 atcaaacatt ttccctgcta gtacatttgc agttccagca caaccaccaa ctacagcagc
     8461 cctagctcca tatatacctg catctggacc ttgtgcacgg cgtaatccaa attcaaatac
     8521 agggtctcct tgagctgcaa agcaaactct agatgcttta gtggcaataa gggactgaaa
     8581 attaactatt gtcaacaatg ctgtttcaat gagttgagct tgatacaaag gtgccttaac
     8641 agtaattaat ggctcattag ggaacatgat agttccttct tcaacagcat atatatctcc
     8701 tgtgaattta aattctttta aaaattttaa gaacttgtca gaaaataaat ttaagttttt
     8761 taagtacttt aaatcttcat ctgagaaatg aagattatct atgtattcaa caacctgatc
     8821 aataccacaa actatagtat accctccatc acaagcgttt tttctaaaaa acatatcaaa
     8881 tacaacaatg tcttcatgaa tattcttttc aaaatagcca tttaacatag taagctgata
     8941 taaatcagta agaagtgtta gatttctcat tatttctctc cttaattatt attaaatatt
     9001 aaaaattgta aaaactccca cacttatact aaaacataat atagtacaac tttcaagtat
     9061 aaagtattac ctaactatca taaaattatt taaagaggag gatgattata ataggaaaaa
     9121 tatggtttta tatggtatcg atagggataa taggaagttt aatttctaaa aatatgggtg
     9181 aattaaataa agttatatta agtgaaacat caaaaggtat agagtttgca ataagcctag
     9241 ctggtatcat ggcactctgg atggggataa tgaatatagc aaaagagtca ggcttaattg
     9301 aaaagatagg tcaaaaatta aatcctataa tgagaagatt atttccttct atcccaaaag
     9361 gtcataaggc aatgtcttat atagttatga atattgcctt aaatatgttg ggtgctggaa
     9421 atggtgcaac agcatttggt ttaaaagcaa tgagtgagct tcaaactttg aataacaaga
     9481 aagatacagc tactaatgat atgattatgt tcatggtaat caatatatcc tctatacaaa
     9541 taattccatt tactataatt aaattaagga tggatatggg agctcaaaac ccaagtgaaa
     9601 taatttttac gactttattt gcaaccataa tatctacagt aatagcgata ataacatgta
     9661 aattttttca aagaagatat agataaaggg gatgtagata catggagata ttttcaaact
     9721 tacttatacc aataataatt ttgtacatag tagtttatgg taaatataaa aaaatagatg
     9781 tgtatgatag ttttgtaaaa ggagcgattg atggattaaa agcagcatgg gatatcctac
     9841 catatataat aggtatattt ctagcaatag gaatattcaa aactggtaaa ggtcttgata
     9901 tgttagaatg gctatttact ccaattgcaa atatgatgag tataccaaag gaattaatag
     9961 gtctaataag tgtaaaacct ttatctggaa gtggtgcttt agggatgtat agtgaacttg
    10021 caaatagagt tggaatagga agtttggtag aaaaaatggg tgccacgatt gtaggttctt
    10081 ctgagactat tttttacaca atggcaatat actatggaag tctgaagata aaaaatacta
    10141 gacatacatt atcatgtgct atgattagcc atgttgcagg tgttatagca gctgttttta
    10201 tttgctatgt aatatttgta taatttgttg acaatatggg aaaaatttta taatataatg
    10261 attatataga aacaaatagt aatataaatt acaagctatg aaaagaagag taaataaagg
    10321 ttttttctat cagagagcta gattaggtga aagctagtgt caaaagcttt attgaagatg
    10381 gtcttggagc tttttatctg agcaagtgtg ctagggtaaa acggttggac acgttatagt
    10441 ctactaaggt atctataaaa tgatttctac atttcatgtg aaacataaac attatagatg
    10501 aattagggtg gtaccgtgaa taatctcgcc cctatgttaa ctcataggag gcgatttttt
    10561 attatttaaa tgaaagaatc atatgattac gaacgataaa aaattaggag gtaaataata
    10621 tgagtaaacc gagtttttat gtaacaacac caatatacta tccaagtgga ggattacaca
    10681 taggacatac ttattcaaca gtagctgcag atacaatagc tagattcaag cgtttctgtg
    10741 gatatgatgt aaaattctta acgggaacag atgagcatgg tgaaaaaatt caaaagaaag
    10801 ccatagagca aggaatgtca gagatagagt atcttgatgg aatgataaaa gatataaagg
    10861 ctttatggaa tactatggat atatcttatg atgattttat aagaactaca gaaaaaagac
    10921 atacagatat aatacaaaag atatttacta aactatatga gcaaggtgat atatataaag
    10981 gtgaatatga aggtagatac tgtactcctt gtgagagttt ctggacagaa tctcaattac
    11041 tagaaggaaa taaatgccct gactgtggaa gagaaacata tttagtaaaa gaagaatctt
    11101 atttctttag attatctaaa tatgaagata gattaaaaga gttatttaaa gatgatagct
    11161 tctgttttcc tgctgctaga aaaaatgaaa tggtagcaaa ctttttagac aaaggattag
    11221 aagatttaag cgtaactaga acgacttttg actggggtat aaaagttcct tttgatgaaa
    11281 aacatgtaat ttatgtgtgg gtagatgcct tatgtaacta tataacagca ttaggttata
    11341 tgactgataa tgatgaagaa tttaaaaaat attggcctgc caatgttcag atagtaggta
    11401 aagaaatagt gagattccat acaataatat ggccagcact tttaatggct ttaggtctag
    11461 aagttccaaa acaagtattt gggcatggat ggatactgtt tgctgatgat aaaatgagta
    11521 aatcaaaagg taatgtggtt tatccagaac ctataataga aagatatgga atagacacgc
    11581 ttaagtactt tttattaaga gagtttgcat ttgggcaaga tggaagttat actcatagaa
    11641 attttgtaac aagaataaat tatgatttag caaatgattt aggaaactta ataagtagaa
    11701 ctgtagctat ggttgaaaag tacaataatg gtataatacc aacagcaaaa gtttcaactg
    11761 attttgatgc tgatttaaaa gagcaagctg tatcaactag agaaaacttt gaagctgaaa
    11821 tggataagat gcaattccat gaagcattag agagtgtatg gaaactagtt agaagaacta
    11881 acaaatatat agatgaaact atgccttggg cactagctaa agatgaagct aagaaagatg
    11941 agttagacac agttttatat aatttatgtg aatctataag aataatcgca acactaataa
    12001 atcctataat gaatgagact gctaataaaa tatatgaaca tataggaata aagggtcaag
    12061 atgatataac tacatgggaa agtacaaaaa catttggtct tattggagaa aatgtaaaag
    12121 tatttaaagg tgaaccttta ttcccaagat tagatgtaga aaaagaaata gaagagttaa
    12181 ctaaaatgtt ctctggaaaa cctccagtag aagaaaaacc tttagagcat aaagaagaaa
    12241 ttactattga tgatttagac aaaatagagc ttagagtagg aaaaatatta agctgtgaaa
    12301 aacatccaaa agcaaataaa ttattagtat cgcaagttaa gattggacca gaaacaagac
    12361 aaatagtatc aggaatagct gaatactata agccagaaga tttagtagga aaagaggtta
    12421 cagtagtatg taacttaaaa ccagttaaat taagaggtgt agaatctcaa ggtatgatat
    12481 tagcagcagg tgatgatgga gacccatatg ttttaccatt tacacaaggt gctaaagatg
    12541 gatgcgaggt tcgttagtag gcaattaaag agtaaggaga ttaaagatgt tatttgactc
    12601 acatgcacat ttaaatgatg aaagttttga tgaagataga gatgaactta ttggttcatt
    12661 aaaagataaa ggtgtagatt tggttgtaaa tccaggagca gatatagata cgtcgataac
    12721 agcaattgaa cttgcgaaaa aatacgactt tatatattca gcagttggag tacatccaca
    12781 tgaggtatca aaactagatg atacagcaat agaaattcta agaaaacttg caactgagaa
    12841 tgaaaaagtt gtagctattg gagagattgg attagattat tattatgatt attctccaag
    12901 agaagaacaa aaagagtggt ttaagaagca aattgaatta gctaatgagc taaagcttcc
    12961 aataataata catgatagag atgcacatgg agatactttt gagataataa agaatactaa
    13021 gagtcctgaa ataggatgtg tacttcattg ttatagtgga aatgtagagt tagctagaga
    13081 atatgttaag atgggttgtt atatatcaat tccaggcacg gttacattta agaataataa
    13141 aaaaactaga gaagtagtaa gagaaatacc tctagaaaga ttattcatag agactgattc
    13201 accatatatg tcaccggagc cacatagagg aaagagaaat aatccttctc aagtatcttt
    13261 tgttgctgat aagatagctc aggaaaaagg tatatcttat gaagaagtat gtagagttac
    13321 aaaagaaaat gcaaagaaat tttttaatat aaaatagaca aattaagtcc tataatggta
    13381 tataaaataa gtcattataa gtatagaatg agggtgcctc aaaatgaatt ttttagttca
    13441 tgaggtactc ttttttatat gaaatcaaat atattttcgt catttcaaca atgaaaaata
    13501 agtttatcct attttgatac agtctatttc tatttattta attcatattt taaaatatat
    13561 ttttttaaca ttgttaaaaa agaatttaca agatattcac tagaattaac tcaactataa
    13621 tattcattta acttaccttg tttattatat tattaaagag aagtaaacga aggacagatt
    13681 aaggaggcta aattttgtat acagttgata gtactaagat attgataaat atgaacttag
    13741 agaaactaga aaagatatac tcaatgttaa agtatacatc aaatgtttct ataattaaag
    13801 aacctaagtt ggctacagta atggaagagt taatgaaaat gtaaaaaata caacatttaa
    13861 tttaggggat atattagtta ctgaatgtag tgtagaggta gatgaaagtt tgggttacgg
    13921 aatagttgca gaaaaaacta ataatttaaa taaagcagta agtttattta cctcaagcac
    13981 tattaaagtt ggtatatatg atgaaagaag tagtgtaaaa gaaaataaaa agcagattta
    14041 gtttcagtag atattaacag tgaatatcca aagcttgtaa gtattattgt aaatggaaaa
    14101 gcagtttata aaagggaggt ataaagattg ttaacagagt tacactgtca tacaaatata
    14161 tcagataata attttactac tgaagaaata ataaaaaaag cagcagaaaa gaatataaat
    14221 atcttagcaa taacaaatca tgacacatta tctggattgg atgaagccat tgtgcttgga
    14281 gaacattatg gaataacaat aataccaggt atagaaattt cagcttatga ttatataaat
    14341 agaaaaagag ttcatattct tggatataac atagatttaa acagtcaaga aataaaaaat
    14401 ttgtgcaatc caatggtatg tgacagacac aaagcatctg ttaagatggt aaataaaatt
    14461 atagatttag ggtacaaaat atctctggag gatgtaaaga aatattcatc aaagactgga
    14521 atatttaaac aacatataat gaaggctctt atagataaag gatatacaga taatatatat
    14581 tcaagtctat ataaagaact gtttcataga ggaaacggta aggtatatgt atctttaaga
    14641 tatgtagatt atagagatgc aataaaagct ataaaaaatt ctggtggaat ttgtgtgtta
    14701 gctcatccgg gtcaaatgga taattttagc gctattgaag agatggtaaa agtaggatta
    14761 gatggaatag aagtatatca tccatctcat aacaaagaac tagaaaaaga atcgctttct
    14821 tatgctaaga agtacaatct agtggtcaca ggaggctcag attaccacgg attttattct
    14881 caacataatt atgaacttgg aagtaagagc ttaaataaag aagatttatt taagtttagg
    14941 ttagctttag gaggggtatt ataaatgtct tttttacaag taaaaaatgt atgtaaaagt
    15001 tatggacaag ttaaggtgtt aaaagatata tctattgata ttgaaaaagg agagtttatt
    15061 tgtttactag gaccaagtgg ttgtggcaaa agcacactgc ttagaataat tgcggggctt
    15121 gaggataaac atggtgggaa aataataata aatgatagag atatgactaa ttcaccacct
    15181 gaaagtagaa attttggtat agtttttcaa tcatatgcat tatttccaaa tatgaatgtt
    15241 tacaaaaata tagcttttgg actagaaaat aaaaacataa gtaaatctaa cattgataaa
    15301 aaagttaaaa aagttctaga agttgtagaa ttaagtggat atgagaaaaa atatccatca
    15361 caattatcag gtggacaaca acaaagggtt gcattagctc gtgcgattgc tttagagcca
    15421 gactttttac ttcttgatga acctttatct gcattagatg caaaagtaag acttaaactt
    15481 agggagcaga ttagaagttt gcatagaaaa ctaggcatta caactataat ggtaacgcat
    15541 gaccaagaag aagctctttg tttagctgat aagatggttg ttatgaatag aggagaaata
    15601 atacaagttg gaactccaaa ggaagtatat aaaaatccag aaacaccttt tgtagctgat
    15661 tttataggaa caataaattt tatagataat ggtattaata agatagctat tagaccagaa
    15721 gacataaagg tagagttcaa tagagattct aaagataaag atataaaaga gggagagatt
    15781 ttggatatag aatttagagg atttaactat agaattacag ttgagtattg ctctaagcaa
    15841 atgaagttag atgtagtttc aaaagttgca gaacaaatga aactgtgtat aggttctaaa
    15901 ataaacttta agatacctaa agaaggaatt gtacaatata aatcagaggg atgtgcttaa
    15961 tagctaggga tgaataaatg ataacatcaa aaaataaaag aagaataagt gatagcacaa
    16021 tacaaaaata tttcttgatt ttttcagtaa ttgtactaat agtatttata ttatttccgt
    16081 tagcatcatt attaaaaaat gcatttatgg atacaagtgg aaattttata ggaattttaa
    16141 actttagtaa atatattgag aatccaagtc ttatcgcatc atttaaaaat tcaatttttg
    16201 tatctatagt ctcatcttta atatcattat gtttagcatt tatatattca tacgcaataa
    16261 caagaacaaa tataaagttt aaaaactttt ttaaaactat gggaatgttt ccactttttg
    16321 caccaactat gttatatggt atttcactta tatacctttt tggaaataag ggaatcttta
    16381 caaccatggg tctggaagta ccactatatg gtccattagg aataattatt tctcagattc
    16441 tatttacatt tccacaagct tttttaatat tatctgtagc actttcgatg gctgattaca
    16501 gactttatga agcagcagat agcttgggtg ctagtgaatt taagaaattt tgcaaaataa
    16561 caattcctgg tataaagtac tcattactta gttcattctt tgtatccttt atacttgcat
    16621 ttacagattt tggagcagct aaggtagttg gaggaaacta caatgtactg gctactgaca
    16681 tatataaaca agtagttgga caatttaaca taccaatggg agctactgtt tctatggtaa
    16741 tgttaattcc tgtactaata gcatttacat tagataagat ggcaagtaag aaacaaggta
    16801 tgaccataac tgcgaagtct atgccatata agattaaaga aaacaagtct agagatagag
    16861 cgtttttaat agtatgtagt cttataacgt tgctaatttt tattttgata tttgtttcag
    16921 ttataggttc agttataaaa ttatggccat ataatttaag ctttacaatg gaacactata
    16981 catttaaagg tattttggga agtggaataa aaacatattt aagcagtttg aaaatatcaa
    17041 ctttaacagc agtatttgga actatatttg tattttttag tgcatatatg atagaaaagg
    17101 tggataagtt tcctaagtta agacaaatag cttacttttt atcaatgata ccaatggcct
    17161 tgcctggctt agttttaggt atatcatata taactttttt taactcagca agtaatccac
    17221 taaatttttt atatggaagt acagcaatac tagttatagt aaatatagtt catttttatt
    17281 cagtttgttt tataacttca aactcatctt taaaaatgtt ggatatagag gtataaagtt
    17341 aaaagaggaa ggctatccag tagaagtagt atttccaaaa gaaggttctg gatgagatat
    17401 agaagcaaat gctcttgtga aaaaagataa tataaaagaa gcctcaaaag tatttttaga
    17461 ttgggcaata agtaaagatg caatgaatga atattctaag aactatgcag ttactactat
    17521 ttctacggga aatccaatac cagaaggatt tcctaaaaag ccattagagc aaatgataga
    17581 taatgattta aaatcagcag cgaaaaatag agaagatata ctaaataaat ggatatcaaa
    17641 atatgatggt aagacagaaa aagagagtta agcctgacaa taaaatttaa tacaaaaagc
    17701 gcctagaata taaattttat atctaggctc ttttgatgtg tttgtttaaa atgcatattt
    17761 ctatataaaa ataaatagtt tagtaccttg attaaattaa ataaaaacct taaaataaag
    17821 agatgtaaag aaaaattaaa aacgaataaa aggtgaattt atatgataaa agaaataata
    17881 gtagtagagg gtagagatga cgttacagca gttaagagag caatagatgc agaacttata
    17941 actacaggtg gatttggatt tccaaaaggt gtaatggagc gtataaaagc tgctaatgaa
    18001 agacgtggtg ttataatatt tacagaccca gattttgcag gtgaaaaaat aagaaaaaaa
    18061 atagcatcag aagttccagg ttgtaaacat gcatttctgc caagagagga agctaaaaag
    18121 gatggagata taggtattga aaatgctact cctcaaagta taatagcagc tttgaataag
    18181 gttagaactg aaaatacaga aaaaagagat gaatttaagc aaattgactt aattagaaat
    18241 ggtcttatag gtaatgaaga agcatcactt agaagagatg cacttggaaa aatacttgga
    18301 atcggatatg gaaatgcaaa acaattttta aatagattga ataattatgg agttgaaaga
    18361 gaagcattta tagatgcatt aaaacaaata taaatgtaat gtttaatata tgaaaaatat
    18421 gtatataaaa taaattgtgg aattacggtt aatttaagat aaattattta taatatatac
    18481 cttattttaa atcaatgtaa atacatataa ataagtataa ttaataaaat taaatatcaa
    18541 aataagttaa aaaggagtaa ataatggata gactatcttc acataatgca acaaaggaag
    18601 tagtacaaaa atataatttt aagttttcaa agtctttagg tcaaaatttc ttgatagata
    18661 gtaatataat tgataaaatc ttatctggag ctagaatcac aggaggagat aatatcatag
    18721 aagtaggacc tggtatagga actcttactc gtgagatggg taaaatagca gaaaaagtag
    18781 tggctataga aatcgataga aatctaatcc ctatattgaa agataccctg tcagatttag
    18841 agaatacaga ggttgtaaat caagatatac taaaagtaga catacaggaa ttagtaaaag
    18901 ataaattaaa tggtggacca gttaaacttg tagcaaatct tccatattat ataactactc
    18961 caatagttat gaagttttta gaggaagata tacctgtaac agacatagta gtcatggttc
    19021 aaaaagaagt tgcagataga atgaatgcta ttccaggtac taaagattat ggtgcattat
    19081 ctatagctgt tcaatactat tgtgatacag agattgtggc aaaagcccca agacatatgt
    19141 ttataccaca accaaatgta gattccacgg ttataggctt acatgtaaga gataaaagaa
    19201 aatatgatgt tcataatgaa gaaatatttt ttaaaacagt aaaagcatct tttggacaaa
    19261 gaagaaaaac tcttcttaat tcgttgggag gtcttggctt tcttaataaa gatgaaataa
    19321 gagaaatatt aaaagaagct aatatagacg aaaaaagaag gggtgaaacg cttagtatag
    19381 aagagttttc agttctttca aatataataa atacaaaagt atcttctaaa tagaaggtac
    19441 ttttttttat tttgttaaca tatattgttt agagaaactg tgttaagggg ggattgcagt
    19501 gggttctaaa agaaagttta aaagagacta tataatacta gaagctaaag acataaactt
    19561 tagatacaaa gaacgagtac tgccaaaagc ttttgcaaaa gttgaagtaa acgatgaaaa
    19621 agcaattatt gcattatatg tagaaaatct aaaatatgtt aaagatgggt ataaagcagt
    19681 agcaataaaa gatgaccatg gattaataga cttaggaaat ataattttaa atgaacaagg
    19741 aagaggtgaa tttgttttag atttagaaga ttatgatgat gagataaaag gtattgcatt
    19801 attgtatgaa agaaatatac ctttaattgg atttaaggga aataagcttg aaaattatga
    19861 ggatatatta ttccaagagg tagaggaaga agaatatgaa gaattggaag aagaagacga
    19921 agaggtagag gataatgagg aaagtaatag agacgaagat attgaagaag aagaatatat
    19981 tatagaagaa tatgatgaag atgagtatga agactattgg gaagatgatg aagatattga
    20041 agaaggagaa ttaaaagcaa gaacggaagt agaagattct agtaatgaag aaatagaaga
    20101 gttcaataat tacaaggaaa tagaaaatta taatagggtt aaattacagg aagaatatat
    20161 agacatagaa tgtgaagagg atgaagaaga ggatgaagaa gaagtttttg aagtaaatag
    20221 agaagatgac aggtttgttt cttcaaaacc tactaaatca aagaaagttc aaaaagagca
    20281 gaataatatt gaaaaaataa aaagagtcaa tggagtaagt caaggagaga gtggaaataa
    20341 acaaaccagt acattactta tgccaagaca aataaaaaaa ggtttgaaat tatttaaaga
    20401 agtaaaacct tttgtaacag attatataga taagacaaga tggtggaaaa tagagataaa
    20461 tcctacaaca ttatgtggat atacaatgcc ttatttagga tatttaaacg tgttgaatta
    20521 tactatgtat agtgatgcag ttttacattc atataaatat agacactatt tatttggagt
    20581 tcagtatgat gaatataata agagaaaaca ttatatttat gctatcccag gaaatagaaa
    20641 tgaacagcca gacaatggaa gcacaggatt tttaagatat caggcttgtg ataatagaac
    20701 aaatagttta gggtattggc tatgttttat agattctaga caaagaaata tagttaggta
    20761 aaaataataa aaataaaaaa atttagactc aatttttatt ttactgaaaa aattaatgaa
    20821 atgcagtaca aaactttaaa aactattgat aatatttaaa tattacacta caatatatct
    20881 tgaaatacct attatattat ggaggaaaat tatgataaac gagcaaagaa ttttaaatga
    20941 gtttcttgaa ttagtacaga tagacagttt atctttaaaa gagggaaatg ttgcgaaagt
    21001 attagttaaa aaattagaag aaataggttg ttcagtagta gtagacaatg ctggtgaaaa
    21061 agcaaatgga gaaactggta atataatagc tacactaaag ggaaataaag aaggaaaaaa
    21121 aatattattt agttcacata tggatacagt aactccaggt ataggggtaa aacccatagt
    21181 ggatgaagta aacggaataa taaaaagtga tggtactaca atattaggtt ctgatgataa
    21241 agcaggtata gcagctatat tagaaggatt aagatatata aatgaaaata acatagaaca
    21301 tactgatata caagttgtat tctctatatg tgaggaatgt ggattagttg gagctaaaaa
    21361 tttagactat ggaaaaatag actctgaata tgcttttata ttagatagtg gtggttctcc
    21421 aggggaaata atagtaaaag ctcctgcaca agatgtaata aatgttaaga tattaggaaa
    21481 gactgctcat gcaggtcttg aacctgaagc tggtataagt gctataatgg tagctgccag
    21541 agcaatagaa aatatgaatt tacttagaat agatgaagaa acaactgcta acataggaat
    21601 tataaacggt ggaacagcta caaatatagt aacaggagaa gttaatatag ttgcagaagc
    21661 tagaagttta aaagaaaata aattagatgt tcaaactaaa cacatggtag agacttttga
    21721 aaaagctgca aaagattttg gagctcaaat tgagatagat gtcaatagag catatacacc
    21781 aatagatgtt agtgaagatt ctgaaataat aaaacttgca aagaaggctt tttcaaactt
    21841 aggtatagaa ggtcacactg aatctacagg aggaggtagt gatacaaata tattaagtaa
    21901 gaatggtatc gaagctataa ctttaggaat cggtatgaaa aatgctcata cactaagtga
    21961 acatatagct ataaaagatt tatatgattc tgctaaaatg gtagtggaga taataaagga
    22021 agcataaatt taaataatag tgtataatat atatatcgcc tagcttaagc taggtgattt
    22081 ttcattttat agaagattga taatctctat tgaaagcaaa gagtatacat ttgcatataa
    22141 gagagtgttt aatttatatt cagatgtagg ttcttaataa tatttagatt gtttcataag
    22201 ggaggaataa atatggaaac aaggtatgaa gaagcaaata aaataacaat acaatcaata
    22261 ctttggaatg tagtattaac aataattaag gttatagctg gtgttattgg taattctagt
    22321 gctatgattg cagatggatt acattcagca tcagatatta taagttctat aggagtatta
    22381 ataggcaact atgtatcttc tagacctgga gatagagagc ataattatgg tcatgagaaa
    22441 gcagagacat tagtgtcatt tgtgttatca atactgctta ttttcgtttc tataacaata
    22501 ggtatagagg caattaaatc tttatttaac ttagatgcat tgagtgttcc atcaatattg
    22561 ccattagtag tatcagttat ttcaatcctg ataaaagagt atcaatatag aataacaata
    22621 aaagttgcta aaaagataaa ttctccagcc ttaaaagctg atgcttggca tcataggtca
    22681 gatgctttat cttcagtagc tgcttttata ggtataggtg gttctatttt agggtttaag
    22741 ccattagacc caattgcatc agttgtagtg gctatatttg tggctaaagt aggaattagt
    22801 atacttataa gttctgttaa tgaattgatg gatgtttcag ttgatgaaga ggagataaaa
    22861 gaattaaaat ttattgtagc agatacagaa ggtgttaaaa atcttggaga tataaagact
    22921 agaaaacatg gagctatggc atatgtagat ttaactatat gtgtggatga agatttgact
    22981 gttaagcaag gtcatgatat tgctactaag cttgaaaaac atataataaa acatatggaa
    23041 tttgtaaaag gcataacagt tcacgttgaa ccatgtacta attgccaagg aaataagtgt
    23101 aataattaat taaaacttaa aaaaaagtgt aaaaagttat ataattgttt tatgattata
    23161 aactctaggg gtggttaaat gagcaaaaag agaaaactaa agaaaaaagc tgcaatattg
    23221 ataggagtgg ttgttttctt tataatatac tttatatcct ttagaatagg aataaatatg
    23281 gaaaaggata aaagtgaagt gccaaagaag gtagaaaaag aagaagaaga taataggtct
    23341 ttaaggactc aaataaaaga aaagaagaag gtttatatat cagataaaaa tatagaaaac
    23401 ataaaaattg aggaagatat gtgggatgaa ataaagttat ttttctcaga ttttaagaaa
    23461 gtaagaaata ctacttcttc ttatgaagca gtctatgaag gctattctga tgatggagtt
    23521 aaattttcta ctgaccttaa cttttttaga gtatatactg taaacaaaga agaatattat
    23581 aaagtgccag ttagttctaa ggatgctttt aagaaacttt tagatgagag tatatatact
    23641 tcgtttgacc ttctaagtca ttataaagat tggaaagagg ttactataac ttatggtaaa
    23701 gaaattaaaa aagttcatag atggaagttt gatgatttag cacataagat gatttcaaaa
    23761 agggttgtag gaaaagttca acctgaaaaa agtaaggaaa gaagtgacta taattttact
    23821 ataaatataa aaggtgataa ttttgatgta aaagttgata caatgggaaa agattatgta
    23881 aaagttactt ctaagaaaaa tgtatcttac tatgaggttc atacaagttt atttgattac
    23941 cttaaaaatg aaatatttga aattggaaag aaataatact ttaagaaggc tatcaaatta
    24001 gatatttgat agcctaaatt gttttattta ttatttttca accaacaagt ctgcaacctt
    24061 ataaattgga ccagcaccag cagttattac taaatcattg tccttaacat tttcacgtaa
    24121 gtattttact atatcttcaa attcttttat atatatggca tctacattgt tttgatataa
    24181 tttctcaacc aagtccttag aatgtatatc tccggggtct ttttctctag cagcatatat
    24241 atctgttatg ataactttat cagcagcata gaatgcttct gaaaattcac ctaaaagaga
    24301 ttttgttcta gtataagtat gtggctggaa gatacaccat aaagtagatt tttttaattt
    24361 tttggcagca gctaaagtag cttttaattc agttggatga tgtgcataat catctactac
    24421 taaagcattc ttataatacc ctttagtttc aaatcttctt ccaacgcctt tataaatttt
    24481 tatatttttt cttatagtct ctaaatcaat accagaaaca agagctgcca ttatagcaga
    24541 agaagcattg tatatgttat gaagaccata tacagaaagt tcaaattctc ctaaatctct
    24601 tccttgatac tctattctaa atattccatg accactattg tcaaagtgaa tatcttttat
    24661 tacagcatca ttgtcagaat ctctaccgta ttttataata gtagctttaa catcatacaa
    24721 tatgtcatca atattttcat catcaccatt tattatgaaa tagccttctg gaggtaataa
    24781 ttttccaaat ttgttaaaag aagctttgat ttcatctatc ccagagaaat aatcaagatg
    24841 gtcctcttca acatttaaaa ctatagatat tttggggtta aaattaagga aactatctac
    24901 atattcgcat gcttcagtta taaagtggtt ggaattacct atcttaacat taccacctat
    24961 catacttaag ttgccaccaa ctagtatagt tgggtctaag tctgcatatt caaagattgt
    25021 agataacata gatgttgtag aagtttttcc atgtgttcca gaaactgcta tagaattttt
    25081 atattccctc atgatttggc ctaaaaaagt cgctctgttc attactaatt tgtttttttc
    25141 tttagcagcc acaagttctt cattgtctgg gtggactgct gctgtataaa caaccatatt
    25201 tacatcatct gatatattat cttttttctg accaatataa atagttgccc cttggtctct
    25261 aagtttgtct agtaggtatg attcgtttga atccgaacct gaaacttgat aacctttatt
    25321 gatacatatt tcagctaatg cactcatgct gataccgcca attcctataa agtgtatatt
    25381 cataataaaa ccacctttct ttttaattaa gtcctacaat atgaactatt tctgtggtat
    25441 aataatataa tgttcaatat tttataatta atatgcatat aatattatat gtattttatt
    25501 tttgaatata ataaaacata agtaatatta taccacaata agaattgtta tgcattagtt
    25561 agtttaacgt tatataaagg tacttgtagg aggaaataat aatgaaattt aaaagaacag
    25621 agagaattgg agcaatagtt aaaatactgt ctgataatcc aaataaaata tatacattaa
    25681 gttattttac aaatcaattt aatgcagcaa aatcaacaat aagtgaagat ttattagttg
    25741 tgaaaaatgt atttgagaag ttacacttag gtaaggtcat aactatctca ggagcagcag
    25801 gaggagtaaa atatatacct aaaacttcta tagcagaaaa tgcagagttt ttaatggagt
    25861 tatgtgagag aatatgtgac aagtctagaa tattatctgg tggattttta tacttgattg
    25921 acataattta tgaccctaca atagctgcta agataggaaa gatatttgca tcaaatattg
    25981 agtatgtaga tgcagattat gttgtgacaa tggaaacaaa aggtatacct atggcactta
    26041 tgactgcaaa agctatgaat ttacctctag ttataataag aaaagatata aaggtatcag
    26101 agggtcctac acttagcatg acatatgtga gtggaaacag ctctaaagtt gaaagtatga
    26161 gtttgccaag aaaagctctt aaaccagata gtaaagttat aataatagat gatttcatga
    26221 gaggtggagg tactataaaa ggaatggtag accttatgaa tgaatttggt gcagaagtta
    26281 taggtactgg agtattcata tctacaacga atccttcaga aaaaatggta aaagattata
    26341 tttcattaat tcaattggat gttaatggag ataaaatagt agtagaacca aacttaaaga
    26401 cttttaaaga tgaatataga aatgaagatt tagatgaaga agaagattca gaatttgaat
    26461 ttgagataga tgaagattaa taaaaaaaat atttagaaaa atatgtaaaa ataaaaagag
    26521 gatatcccta gt
//
LOCUS       DAFNNB010000035        26407 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0035, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000035 DAFNNB010000000
VERSION     DAFNNB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0035"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            208..304
                     /locus_tag="L3M60_001340"
     tRNA            208..304
                     /locus_tag="L3M60_001340"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:242..244,aa:Sec,seq:tca)
     gene            455..1162
                     /locus_tag="L3M60_001341"
     CDS             455..1162
                     /locus_tag="L3M60_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HBN6226020.1"
                     /translation="MENNFTTRTSFLVGDDGIEKLNNSNIIVFGVGGVGSFTVEALAR
                     AGVGNITIVDFDDVDITNINRQIPALHSTVGRYKVDVMEERILDINPNINIKKIRSLY
                     NQDTSDEILTERYDYVVDAIDMVSSKIHLIETCEKKGLKIISSMGMGNKLDPTKIVVT
                     DIHKTSTCPLAKVMRKELRDRGIKKLKVVYSTEQPIELKKKVMNGRKVTPGSVSFVPS
                     VGGLIIASVIINELLGQ"
     gene            complement(1304..2140)
                     /locus_tag="L3M60_001342"
     CDS             complement(1304..2140)
                     /locus_tag="L3M60_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6226021.1"
                     /translation="MRNKIFLSILIIISLLIGCDKKSLLSIHMIDVGQGDSILVQTPT
                     NKNILIDGGDEYSKNIINSYLRQKRIKTIDIIIATHPDSDHIGSLDNVIKKFNVNSIY
                     MPEQSTNSESYQNLINSCTDKNLAIQYLYKDDVLNIDNNINIYVLSPSYIQEESNLNS
                     IVFKLTFNNNSFLFMGDAEEENEKEILRSFELNNINFIKIGHHGSNSSSSLEFIKKIN
                     PDIAAISCGYKNQYGHPHKEVIDNLKQSHVSIYRTDRIGDIVFYSDGEIIFTKYNYEV
                     DT"
     gene            2327..3232
                     /locus_tag="L3M60_001343"
     CDS             2327..3232
                     /locus_tag="L3M60_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2156 domain-containing protein"
                     /protein_id="HBN6226022.1"
                     /translation="MFFKDIELNSKKELDPYFDLVDYEACEYCFSTLYMWQHVYKTGY
                     YIGEDFAVLVGEYEGDSFSILPLAKKDKLPEVVDFVLEYFSKNNKKIYLRGITTEVVE
                     FLKEKYPDRFEYIEERDLFDYIYDAESLRTLAGKKNQKKRNHINYFLKEYAGRYESKL
                     LDKENFDECLVLMKEWESNKEENNEFDESMDDELIGIKKIFNHYDILKDKVKVFGVYV
                     DGKLEAFSIGELLNPNMALIHIEKANPDIRGLYPFINQQFLVSEFKDVEFVNREEDLG
                     IEGLRKAKLSYHPCRFVEKYSVREA"
     gene            3241..4425
                     /locus_tag="L3M60_001344"
     CDS             3241..4425
                     /locus_tag="L3M60_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6226023.1"
                     /translation="MEIRYAKEEDIESIKEIWSYCFNDTESFMKYYFNDKYKSENTVV
                     ALDEGKIISSLQLNQYKLLLNNKVYNTSYVVGVSTLPEGRGTGYMSKVIKFTLNELYK
                     KGQLVSILMPIDYRLYRRFGYEHCYDQIEYTINTDDLKNFKSSGKMIKSNLSQIDDLI
                     RIDRAFLNEVNGNVLKDEHYYKNLFKEIQSEDGFLYIHEGNEKDGYIVYFINEDKMFV
                     RELFYKNIDALKSMLKFIYNHNTQCKIVTISTPIIDKIRFILDNPKDSEIKIKPFMMG
                     RIINVKKFIEDVDIENDINSSFNLLIEDKFINENNGLFKISIQNKKVSVEQLYKKDEE
                     KPQEDFDVKLDINTLTQLAFSYIDVNEAIFLNDIKDVSEETLEILNCIFSKKNNYINE
                     YI"
     gene            4540..5841
                     /locus_tag="L3M60_001345"
     CDS             4540..5841
                     /locus_tag="L3M60_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HBN6226024.1"
                     /translation="MGVIIIQIRRPLLIILVFVIAISFIITNNTKNNEEINDKIITIQ
                     GIVKGKIEKKRYNQYKVGVFLINDYTKNKNLKIGQKVNITGKFKSLDKMKYDDFDYGR
                     YIKSTGYKGIVYINNYKIIDKNKFYSLIGEIKFYISKTYRYLYKKNSDFINSILLAEV
                     ENLSEEQKEIFTRTGTSHVISISGLHTGILCIIISFLLRGINKLYKLLILSIFITLYC
                     IMVGASPSIIRSIAFVMIFYLSIFIDRKKDGISALSLIGIILIMNNPYVIYNVSFQLS
                     FLATLSILIFYNKINSIIKLSMVSLTISSNILTLPIIYYTFKGIPLLSIIGNLIIVPF
                     VGVIIYLSIASLIVFKVSVVIAKIISFFNSTLIESIFFLLEKISNLSFAYINIEKPKF
                     YIVIIYYIGVFFYIFYIEGKEIKEQENELQGYYKECKREKF"
     gene            5795..6838
                     /gene="holA"
                     /locus_tag="L3M60_001346"
     CDS             5795..6838
                     /gene="holA"
                     /locus_tag="L3M60_001346"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBN6226025.1"
                     /translation="MNYKDIIRNVKEKNFEKMYLFYGREYYLIENAIKAFKDSLNEGM
                     LDFNLDIIDGKEIVLNQLISSIETLPFMDDRKIVIIKDFELLKGKKKNFTDSDEKYLI
                     EHLDNIPDTTTIVFVVYGDVDKRKSLVKKIGSNGIVFDCDKLSDMDLFKWIKKSFALN
                     DVIIDNSQIMYFIEQEGYRDKSSEKTLSDLENEINKISSFVGKGNNVTNDVIDKLSQK
                     KVENDIFKLIDYIGEQNASNAMKILNDMIQEGESVLGIFSMIARQFKIIMQVRQLQLD
                     GYSTKLIADKLKMHQFVVGKALKQTKNFSDDIIVEILNYILESDYKIKTGLIRDTLAV
                     EMLVSRYCKREAI"
     gene            complement(7028..7294)
                     /gene="rpsT"
                     /locus_tag="L3M60_001347"
     CDS             complement(7028..7294)
                     /gene="rpsT"
                     /locus_tag="L3M60_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBN6226026.1"
                     /translation="MANIKSAKKRISVIEKKTALNRVRKSQIKTAIRRFEDAVAAGNR
                     EDAVAKFQYAQKRIYQVASKGTIHKNAAARKVAKLAQKLNGMNA"
     gene            7616..8245
                     /locus_tag="L3M60_001348"
     CDS             7616..8245
                     /locus_tag="L3M60_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6226027.1"
                     /translation="MNLIKNYKNTEDTADKLISYIDENCSGHLFALCGVSLGGQIVME
                     LLSRKPNITKKAIIDGSICYPRPLMARFCITGIRFFGGLLFSKKACRFQIAMMPKLLP
                     ENMQYPDEIKAYYMTDMPHVRKETFYNMYRTYMMNYILKENVKKTTAQVMYWYGEKEM
                     KCVKKSAKMFQSYVPSCEIYEAKGYNHGYLSLYLPNEWLEIAEPFFQKE"
     gene            8587..9540
                     /locus_tag="L3M60_001349"
     CDS             8587..9540
                     /locus_tag="L3M60_001349"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HBN6226028.1"
                     /translation="MINVRTDLALEASEMCEKSQEGSSIPGVKIETKELENCIVTKVE
                     VIDEQGSEIMNKDIGKYITLESNLMKFDDDESREEMISYLKDELVDIFGQDENKKTLV
                     IGLGNRNITSDALGPKSVSKTLVTRHLFKNYNKDYDDDFTEVSALSPGVMGVTGIETS
                     EIVKSLVEKVKPDRVVAIDALASRKMERVNSTIQISTAGISPGGGVGNTRKSLTKETL
                     GVDVIAIGVPTVVDAATLTIDVLDMAIDNLIAQSEETESFYEMLKKLKEEEKYHLIKD
                     SLDPYDKNLIVTPKDIDDTIENLSIIISEGLNRSLHPGRLV"
     gene            9558..10577
                     /locus_tag="L3M60_001350"
     CDS             9558..10577
                     /locus_tag="L3M60_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HBN6226029.1"
                     /translation="MFKKCIKVVTLTFILACILPGKSLALNQDDFLKFLVNSSYPEAK
                     VEGNDTEDKKNNKNKETSKENKEESKEEKTKSKDASKVDNKKESEKEYIKLYVGKENV
                     PDIESKNSDTTETNATSSSDYKDDLRVTKENPRILIYHTHGCETYSNSPDGNYHSRDK
                     KNSVMEVGSALTSALDSKGWGVVHTTKYHDYPSYNNSYASSLKTIQSILPKYNSVDIA
                     IDLHRDARDLTNPATKEKDHLKYTTMINGERVSKFFFVVGGKNTNRKQLRALAEDITA
                     FAEKKYPGLVSPIVEKDYARFNQFAVKNHMLVEIGNNATSVEESKATTKYLAEILDEY
                     FKQKN"
     gene            10608..10859
                     /locus_tag="L3M60_001351"
     CDS             10608..10859
                     /locus_tag="L3M60_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226030.1"
                     /translation="MSRLEKNIESKSKLRKYKYIMRIMFLITMIICTSVCVFIVDSNA
                     KSMLGMKQYDFPNIKFDITKFDFDIPNFDIHEIIRKFNK"
     gene            11141..12946
                     /gene="lepA"
                     /locus_tag="L3M60_001352"
     CDS             11141..12946
                     /gene="lepA"
                     /locus_tag="L3M60_001352"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBN6226031.1"
                     /translation="MDNKQSRTRNFSIIAHIDHGKSTLADRLIQQTGLVSERDMKSQL
                     LDNMDLERERGITIKLQNIRLMYKAKDGNEYYLNLIDTPGHVDFNYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYLAIDQDLEILPIINKIDLPSARPEEVKNEIEDLIGLDSS
                     EAPLISAKTGLNIEDVLEDIVKNVPPPKGDNEAPLKALIFDSYYDAYKGVVAYVRVFE
                     GTVKKGMTIKMMNTNKKFEVTEVGVMAPGQTELSELSAGDVGYIAASIKDIRSCRVGD
                     TITDSNNPTEEPLPGYKKATPMVYCGIYPGEGEKYENVRDALEKLQVNDAALEYEAET
                     SAALGFGFRCGFLGLLHMEIMQERLEREFNLDIITTAPSVIYRVTKMDGEVVMIQNPA
                     NLPEPSEIKMIEEPIVKGDIIVPKDYVGVVMELCQERRGNMLNMEYIDERRVMLHYDL
                     PLNEVVYDFFDALKSRTRGYGSLDYEVKGYVASTLVKLDILINKEQVDALSFIVHETR
                     AFPRGKAMCEKLKGEIPRHQFAIPIQAAVGNKVIARETISALRKDVLAKCYGGDISRK
                     KKLLEKQKEGKKRMRQIGSVEVPQKAFMSVLKLDE"
     gene            13095..14432
                     /locus_tag="L3M60_001353"
     CDS             13095..14432
                     /locus_tag="L3M60_001353"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HBN6226032.1"
                     /translation="MINIEIPKKVDYIIKELEKNGYEAYIVGGCVRDCLLERVPNDWD
                     ITTSARPEVVVELFEKTIPTGIQHGTVTVMVEHEPFEVTTYRIDGNYSDGRHPDSIEF
                     TNNIVKDLSRRDFTINSIAYNSETGLVDPFNGYEDIQNKYIRCVGNPVDRFEEDALRM
                     LRAVRFSAQLNFNIDEGTEQSIHKKSDLIKNVSIERIQTEFNKILVSDSSKLNLLKST
                     GLLKFIIPEICELEDVTQHNPYHIYDVQKHTLIATEVIEDELYLKLTMLFHDLGKKVT
                     KTTDKNGVDHFYTHSRESVKIAKKILKRLKYDNYTINKVLILIQYHDYRIEPKRKIIK
                     KLLNKLEDVELFEDLIKVNWADTLAKNPKYAKQKILNLIECEKEFKHIINQKECFNLK
                     DLAINGKDLISIGIKPGKDIGHILNNMLEIVINNPELNEKEILKEKALNIYTF"
     gene            14665..15978
                     /locus_tag="L3M60_001354"
     CDS             14665..15978
                     /locus_tag="L3M60_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN6226033.1"
                     /translation="MHTQLQKTIGLSAALSTVVGMVIGSGVFFKPQAIYTTTNGAPGL
                     GIIAWLLGGFITITAGLTATEISAAIPKTGGMMIYIEEIYGEKLGFLTGWMQTVLFFP
                     GTSAALGVIFAQQASELLGMSPNNMANVLPIAIGVILFLALLNIIGSSLGGKVQTVAT
                     IGKMIPLILIIIFGFIKGQSSEVLNPFVGDGVNASNALGQVLIATLFAYDGWINVGAI
                     SGEMKSPEKDLPRAIVGGLSLVMAVYIIINIAYLWVVPASELATVTSPATLVATRLFG
                     NIGGKVITMGILISVFGTLNGYLLTGSRIPYTLAEMGTLPASKALLKVNSGGSPVNSI
                     LLITALACVYALSGQFNLLTDLTIFSIWVFYVLTFIGVMRLRRQKPDLYRPYRVPLYP
                     IIPIIAILGGLFVIINQILTSTVISLGGIFITLLGLPVYYYMKKR"
     gene            16150..17325
                     /locus_tag="L3M60_001355"
     CDS             16150..17325
                     /locus_tag="L3M60_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBN6226034.1"
                     /translation="MLGLYVHIPFCVKKCKYCDFNSYKMDIDLKKRYIEDLKIEMELY
                     SNKLYKDNKYKNKECCSLNKNDKITSIFVGGGTPSILTSDEIREVFMSIKEMFYIDDN
                     AEITIECNPGTLTLEKLKTMKDIGINRLSIGLQAIQEKHLNFIGRIHTYEEFEKNYKD
                     ALSVGFKNINIDLMYSLPNQTLCDWKETLEKVVHLNPTHISAYSLILEEGTELYNMYE
                     SNKFELIDENIDIEMYEYTINYLMSKGYNQYEISNYSKEGFNCKHNILYWECKPYIGI
                     GAGASGYINENRYNNVESLEDYHLSLVKREKPIQENEILSEKDMIEEKIFMGLRMNKG
                     IKFEDFKKKFGIDFKDKYSKQIEMLSARKLINQSFEGIQLTQKGREISNSVFIEFME"
     gene            17428..18447
                     /gene="hrcA"
                     /locus_tag="L3M60_001356"
     CDS             17428..18447
                     /gene="hrcA"
                     /locus_tag="L3M60_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HBN6226035.1"
                     /translation="MELNERKLNILKAIVKDYIETAEAIGSRTISKRHDLGVSAATIR
                     NEMADLEELGYLIQPHTSAGRVPSEKGYKLYVNSLMSKSELDDNDKILIEQCMNHNIN
                     HIKELIHETSKLLSQLTNYTTVAVTKSLVNQSVIKHIQLVAMNDNNILLIVVTDKGDL
                     KKANLTTNVYLDQSKLNLISDNLTRKLLGKSITDLDDNLIAFIKYEISEYSGLIDELL
                     NALNSNMKEEDFSLSLNGATNIFSYPEFNDVLKAKSFLNMLEKKETIADIIKSKGIQK
                     DNLNIIIGSDNDCELAQDCSIVTATYNVDRDLVGRISFIGPTRMDYARIYSIINYMSL
                     LINRK"
     gene            18477..19097
                     /gene="grpE"
                     /locus_tag="L3M60_001357"
     CDS             18477..19097
                     /gene="grpE"
                     /locus_tag="L3M60_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBN6226036.1"
                     /translation="MDKKNEQQEVREENDTSINQESETQVELEEEVVNEECETSNEKT
                     DEKEVDDENVTDINSKLAEKKLQNELDELNDKYQRLQAEYANYRRRTQQEKETIGVFA
                     NEKIITELIPVIDSMERALDACEDKEDTMYKGISLVHKQLIDTLVKFGVEEIEAESKE
                     FDPNLHLAVMQESVDGIEANQIVMVLQKGYKLGTKVVRPSMVKVSC"
     gene            19189..21036
                     /gene="dnaK"
                     /locus_tag="L3M60_001358"
     CDS             19189..21036
                     /gene="dnaK"
                     /locus_tag="L3M60_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBN6226037.1"
                     /translation="MGKIIGIDLGTTNSCVAVLEGGEAQIIANSEGMRTTPSVVAFTK
                     DGERIVGEPAKRQAVTNADKTITSIKTHMGTDYKVNIDGKSYTPQEISAIILQKLKSD
                     AESYLGQTVTEAVITVPAYFTDAQRQATKDAGRIAGLDVKRIINEPTAAALAYGMDKL
                     DQEKKILVFDLGGGTFDVSILEIGDGTFEVLATAGNNRLGGDDFDQIVIDYLAEEFKK
                     AEGVDLRNDKMALQRLKEAAEKAKKELSSTMSSNINLPFITATAEGPKHLNIDLSRAK
                     FEELTRGLVEKTMEPTKTALQDAGLSTGDIDDVLLVGGSTRIPAVQEAVKKFIGKEPH
                     KGINPDECVAAGASIQAGVLAGDVKDLLLLDVTPLSLGIETMGNVMTKIIERNTTIPT
                     KKSQIFSTAADNQTAVDIHVLQGERSMAYDNTTLGRFQLTDIPPAQRGIPQIEVTFDI
                     DANGIVNVSAKDLGTGKEQKITITSNTNLSEAEIEQKIKEAEMNAEADKQKKEKIEAF
                     NQAESTIYQTEKTLNELGDKISSSEKEEIEKAIADLKTVKDNQDATAEELKKATDEVM
                     TKFQKVSQEMYQKAAQEQQAAQGAEQAQDNGPKDDNVVDADFKEVDEDK"
     gene            21247..22401
                     /gene="dnaJ"
                     /locus_tag="L3M60_001359"
     CDS             21247..22401
                     /gene="dnaJ"
                     /locus_tag="L3M60_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBN6226038.1"
                     /translation="MSTKRDYYEVLGISKGAEAQEIKKAYRKLAMKYHPDRNPGDKEA
                     EEKFKEINEAYEVLSDDTKRKTYDQFGHDGLNGQGGFGGQGGFGGQGFGGFEDMFGDI
                     FGDMFGGSFGGGRTRRRGPQRGADIRQSVTIYFEEAAFGKKMSIKVNRSEECEECNGT
                     GAKPGTSKKTCSTCNGTGQVRTVQRTPFGNIASSRPCSTCNGTGEVIESPCSKCHGTG
                     NTRKVKTIEVDIPAGIDDGQMIKLSGQGEVGEKGAPRGDLYIVVNVKSHPLFTRDGND
                     IYFEMPITFVQATLGDEIEVPTLDGKVKYSVPEGTQTGTVFRLKEKGIPRIRGNSRGD
                     QYVKVVVEIPKKLNDKQKELLREFAKECGSNVHEKKKTFGQKIEDMFKKK"
     gene            23376..24311
                     /locus_tag="L3M60_001360"
     CDS             23376..24311
                     /locus_tag="L3M60_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBN6226039.1"
                     /translation="MYYIGVDIGGTGIQAGVVDNYGKIIFRSECKTVIEKGFEGILND
                     IKIMIYKLLEDNKLTMSDIKSIGFGVPGFINKEGLVTCVNLKWNKKAFNKELKRRFPD
                     VEIHGENDATVAALGEAKFGSMKGANVGVLYTLGTGVGGGIVINQKVFSGAHGLGSEI
                     GHQIIGENYFNCNCGNNGCVETFCSATAIIKYSQKLIEEGEKSRILDLAEGNLENVNA
                     KMVFDAYRENDLVAIKVINRFKEYLAKTFANTINSLDPEIISIGGGISKSSDIILDGI
                     EDLVRKFVLYKTEDIATITCATLGSDAGIIGAAFL"
     gene            24344..25717
                     /locus_tag="L3M60_001361"
     CDS             24344..25717
                     /locus_tag="L3M60_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6226040.1"
                     /translation="MSKTNARRTLFLIAIGSGTMLNPLNSSMISLALHSIQNDFHISF
                     TTVSWIVSAFYLASAIAQPVSGKIGDLIGRKVLFLSGLMLVILSASIVPLVQSFFILI
                     FIRVIQAIGTSTLYPSGVALIQNNIKERQSSALAVLTIFASTTAGLGPTLGGLLLDLG
                     GWHAIFLVNIPVVLVSICLGYFLFPKEVKEKKSIKETLKNVLSRLDIIGILLFSIAMI
                     FILLFLLSMKESFNLEQLVFGIILMYLFIWHELRTKLPFIDIKLFVSNPKLSKVYLQF
                     IILNFFNYILFFGLPSYFQDALHYSAKSTGLFMLFMSGIGIFISPLTGRWIDKSGTRF
                     PLVTSSIFMFISAVLLSVVFIHIPVIGKGIILSLAGISYGVGNVALQSSMFEESPRDS
                     IGTASGLFQTSRYLGSILATVLLGMVFEVNITSEQFQILGYVMVVLGTISFLLNFILK
                     KELRKVE"
     gene            complement(25905..>26407)
                     /locus_tag="L3M60_001362"
     CDS             complement(25905..>26407)
                     /locus_tag="L3M60_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6226041.1"
                     /translation="FIVNSKYNLDINVTTQNIIETETYQSTYIFYLGKLDSKADFDFR
                     LQLCSDLLPHIIKDNAENKFLNLFDLIRYKTLDLINEDNILNKLIDFNKIKSIDEELL
                     YTGLKFINNDLNISKTSTSMFLHRNTLVYRLEKINEILGFDLKNFENAMIFYLSVKSY
                     FLYKKI"
BASE COUNT        10478 a         2776 c         4554 g         8599 t
ORIGIN      
        1 ttgattatat agagaagaaa ttctttatag attcttaaaa tataattttc agttttttat
       61 tttatatata ataaaaaagg taaattttaa ccatgtatag ataaaaccta tttaaattat
      121 aatttactat cagataaatc tataaaaatt gttgcaaaga gtcaaaatat atgatattat
      181 agatttcgtt gagaaggatt tcattatggg agtggatggg tgccggtgtg ccctctagtc
      241 ttcaaaacta gtatgagggg ttaagagcct cttaggtggg ttcgattccc acgcattccc
      301 gccaatatgt aaaaggtatc ttttaattaa aagatacctt tttaaattta ttttaagata
      361 gtttattttt aataatatat aatattttgt attatataat gtaaattttc taatactatt
      421 atatatttag ataaaaatct gggagtgtgg aaagatggaa aataatttta cgacgagaac
      481 tagttttcta gttggagatg atggaataga aaaacttaat aattcaaaca taatagtatt
      541 tggagttgga ggagttggta gttttactgt tgaagcttta gcaagagctg gagtaggtaa
      601 tattaccatt gtggattttg acgatgtaga tataacaaat ataaatagac aaataccagc
      661 actacattca actgtaggaa ggtataaagt tgatgttatg gaagaaagaa tattagatat
      721 aaatcctaat atcaatataa aaaaaataag aagtctttac aatcaagata ctagtgatga
      781 aatactgact gaaagatatg attatgttgt ggatgcgata gacatggtaa gttccaaaat
      841 acatctaatt gaaacttgtg agaaaaaagg attaaaaata ataagttcta tgggtatggg
      901 aaataaatta gacccaacca aaatagtcgt tacagatata cacaaaacaa gcacttgtcc
      961 tctagctaaa gttatgagga aagaacttag ggatagaggt atcaaaaagt taaaagtagt
     1021 atattctact gaacaaccaa tagaacttaa gaagaaagta atgaatggta gaaaggtaac
     1081 acctggtagt gtatcattcg taccatctgt aggaggttta ataatcgcat ctgtaataat
     1141 aaatgagcta ttaggacaat aagttttaaa tattttaatt tataaatttt atatataaac
     1201 ttgaaggcta gaagaattaa tataaaatag ttttatatta attcttttag cctaattatt
     1261 ttacattacc ttattttatg tagttgtagt tttatttttt tgattatgta tctacttcat
     1321 aattatattt tgtaaatata atttctccat cactataaaa aacaatatct cctattctgt
     1381 ctgttctata aattgataca tggctttgtt ttagattatc aataacctct ttatgtggat
     1441 gtccatattg attcttatat ccacatgata ttgcagctat gtctggatta atttttttta
     1501 taaattctaa agatgatgaa gaattacttc catggtgacc tatttttata aaattaatat
     1561 tgtttaattc aaaagaacgt aaaatttctt tttcattttc ttcttctgca tcacccataa
     1621 ataaaaaaga gttattgtta aaagttaatt tgaaaactat agaatttaaa ttagattctt
     1681 cttgtatata tgatggactt aatacatata tatttatatt gttatctatg tttaaaacat
     1741 catctttgta gagatactga atagctaagt ttttatccgt acaagaattt attagatttt
     1801 gataagattc actattagtt gattgttcag gcatatatat ggagtttaca ttaaattttt
     1861 ttataacatt atcaagactt cctatatggt ctgaatctgg atgtgttgct attataatat
     1921 caatagtttt tattcttttt tgtctaagat aactattaat tatattttta ctatattcat
     1981 cacctccatc aattaatata ttcttatttg tgggcgtttg aactaaaata ctatcacctt
     2041 gtccaacatc aatcatatgt atagatagta gagatttttt gtcacagccg attaaaagtg
     2101 atattataat aagtattgat aaaaaaattt tatttctcaa taaaaaatca ctccattcat
     2161 tacatgttta tataaattta tgatgggtaa tataagatat atatggctta ttatatttta
     2221 tattttaaac aatataaatg aaattaatac tataataaaa ttggagtttt ttatatatac
     2281 aaaataaaat ttttaaaatt aagcaataaa aaggcaggag gattttatgt ttttcaaaga
     2341 tattgaacta aattcaaaaa aagagttaga cccatatttt gatttagtag attatgaagc
     2401 ttgtgagtac tgttttagta ctttatatat gtggcaacat gtttataaga caggatatta
     2461 tataggtgaa gattttgccg ttttagttgg ggaatatgag ggagatagct tctcaatatt
     2521 accacttgct aagaaagata aacttccaga agttgtagat tttgtccttg aatatttttc
     2581 taaaaataat aaaaaaatat acttaagagg tattactact gaagttgtag aatttttaaa
     2641 agaaaaatat ccagatagat ttgagtatat agaagaaaga gatttatttg actatattta
     2701 tgatgcagaa agtttaagaa ctctagctgg taaaaagaat caaaagaaga gaaaccatat
     2761 aaactatttc ttaaaagagt atgcaggtag atatgaatct aaattattag ataaagaaaa
     2821 ctttgatgag tgtctagtat taatgaagga atgggaaagt aataaggaag aaaataatga
     2881 atttgatgaa agtatggatg atgaattaat aggtatcaaa aagatattta accattatga
     2941 catattaaaa gataaggtaa aagtatttgg tgtttatgtg gatggaaaat tagaagcttt
     3001 tagtatagga gaacttttaa atccaaatat ggcattgatt catatagaaa aagctaatcc
     3061 agatataaga ggtctgtatc catttataaa tcaacagttt ttggtaagtg aatttaagga
     3121 tgttgagttt gtaaatagag aagaagattt aggtattgaa ggattaagaa aagctaaact
     3181 ttcttatcat ccttgtagat ttgttgaaaa gtacagtgtt agggaggctt agtagactat
     3241 atggagatta ggtatgctaa agaagaagat atagaaagta taaaagagat ttggagttat
     3301 tgctttaatg atactgagag ttttatgaaa tattatttta atgataaata taaatctgaa
     3361 aatactgtag tagctttaga tgaaggaaaa attatttcct ctcttcagct aaatcagtat
     3421 aagttactat taaataacaa ggtatacaat acatcatatg tagtaggcgt ttctacactt
     3481 ccagaaggaa gaggaactgg atatatgagt aaagtaatta aatttacttt aaacgaattg
     3541 tataaaaaag gacaattagt ttctatattg atgcctattg actataggct ttatagaaga
     3601 tttggatatg agcattgtta tgaccagatt gaatatacta ttaacacaga tgatttaaaa
     3661 aattttaagt caagtggaaa aatgataaag tctaatttat cacaaattga tgatttgata
     3721 cgaatagata gagctttttt aaatgaggtt aatggtaatg tgttaaaaga tgaacattat
     3781 tataaaaact tatttaaaga gattcaaagt gaagatggtt ttttgtacat tcatgaagga
     3841 aatgaaaaag atggatatat agtttatttt attaatgaag ataaaatgtt tgtcagagag
     3901 ttattttata aaaatataga tgcccttaaa tcaatgctta aatttatata caatcacaat
     3961 actcaatgta agatagttac tatatctact cctataatag ataagataag atttatatta
     4021 gacaacccta aggatagtga gataaaaata aaacctttta tgatgggaag aattataaat
     4081 gtaaagaagt ttattgaaga tgtagatatt gagaatgata ttaatagctc atttaattta
     4141 ttaatagaag acaagtttat aaatgaaaat aatggtttat ttaaaatttc tatacaaaat
     4201 aagaaggtat ctgtagagca attatataaa aaagatgaag agaagcctca agaagatttt
     4261 gatgttaaat tagatataaa taccttaaca cagttagcat tttcttatat agatgtaaat
     4321 gaagctattt tcttaaatga tataaaagat gtttcagaag aaacactaga aatacttaat
     4381 tgtatttttt ctaaaaaaaa taactatata aatgaatata tataaataga gtacgttaaa
     4441 agattataaa aatcttttga cgtatttttt tgtttatata ataatttacc ttgtttaaaa
     4501 attttctatt tgaaaataat ctaaataata tgcttttata tgggagtgat tataattcaa
     4561 ataagaagac ctttattaat tatattagtg tttgtgatag ctatatcatt tatcattaca
     4621 aataatacaa aaaacaacga agaaattaat gataagataa taactataca aggaattgtc
     4681 aagggaaaaa ttgaaaagaa aagatacaat cagtataagg taggagtttt tttaataaat
     4741 gattatacaa aaaataaaaa tttgaagatt ggtcagaaag ttaatattac aggaaaattt
     4801 aaaagtttag ataaaatgaa gtatgacgat tttgattatg gaaggtatat aaaaagcact
     4861 ggatataaag gtatagtata tataaataac tataaaatta ttgataaaaa taaattttat
     4921 tctttaatag gagaaataaa gttttatatt agcaagactt ataggtactt atataagaaa
     4981 aactcggatt ttataaattc tatactatta gcagaagttg aaaatttgag tgaagaacaa
     5041 aaagaaattt ttaccagaac aggaactagc catgtaatat ctatatcagg tcttcataca
     5101 ggaatattat gtataattat atcctttcta ttaagaggta taaataaatt gtataaatta
     5161 ttaatactgt cgatttttat aactttatat tgtataatgg taggtgcgtc tccatccata
     5221 attaggtcta tagcatttgt tatgattttt tatttatcaa tatttattga tagaaagaaa
     5281 gatggaatat cggcattgtc tctaattggg ataattttaa ttatgaataa cccctatgtt
     5341 atatataatg taagttttca attgtctttt ttagctacac tttctatatt aatattctat
     5401 aataaaataa atagtattat aaaattaagt atggtatcat tgacaatatc atcaaatata
     5461 ttaactttac ctataattta ttatacattt aaaggtatac cattactttc aattatagga
     5521 aatttaatta tagtaccatt tgtgggagta ataatttatt taagtattgc aagtttaatc
     5581 gtatttaaag taagtgtagt aatagctaaa attatatcct tttttaatag tacattgata
     5641 gaaagcatat tttttttact agaaaaaatc agcaatttaa gttttgcata cattaatata
     5701 gaaaaaccta aattttatat tgttatcatt tattacatag gagtgttttt ttacatattt
     5761 tacatagaag gaaaagagat aaaggagcaa gagaatgaat tacaaggata ttataaggaa
     5821 tgtaaaagag aaaaattttg aaaaaatgta tcttttttat ggaagagagt attatttaat
     5881 tgagaatgca ataaaagcat ttaaagatag tctaaacgag ggaatgcttg attttaattt
     5941 agatataata gatggaaagg aaatagtctt aaatcagctt ataagctcta tagaaacact
     6001 tccatttatg gatgatagaa aaatagtaat aataaaagac tttgaactat taaaaggaaa
     6061 gaaaaagaat tttacagata gtgatgagaa atacttaata gagcatttag ataatattcc
     6121 agacactaca actatagttt ttgttgtata tggtgatgtt gataaaagaa agtctctagt
     6181 taagaaaata ggaagcaatg gaatcgtttt tgattgtgac aagctttctg atatggattt
     6241 atttaaatgg ataaaaaaat cttttgcatt aaatgatgtt ataatagata actctcaaat
     6301 aatgtacttt atcgaacaag aggggtatag agataagagc agtgaaaaga cattatctga
     6361 tttagaaaat gaaataaata aaataagttc atttgttggt aaagggaata atgtaaccaa
     6421 tgatgtaatt gataaattat ctcaaaaaaa agttgagaat gatatattca aattaatcga
     6481 ttacattgga gagcaaaatg cttctaatgc aatgaaaata ttaaatgata tgatacaaga
     6541 aggcgaatct gtattaggta tattttctat gatagcaaga caatttaaaa taattatgca
     6601 agtaagacag ttacaacttg atggatattc aacaaaactt atagctgata aattaaaaat
     6661 gcatcaattt gttgtaggaa aagcattaaa gcaaactaaa aatttttctg atgatataat
     6721 tgtggaaata ttaaactata tactagaaag cgattataaa ataaagacag gccttataag
     6781 agatacctta gcagttgaaa tgttggttag tagatactgt aaaagggaag caatataaaa
     6841 aatctatata ttaataaaaa ataaatagca agatatgtta tagtttagag tagatgtaag
     6901 ctaaattaag tttaagtata tacaatcaac tataatatgt ctaactattt attttttatt
     6961 gtataaattt acaaaataaa aaacccctac ataggggtta ttatatataa gaaatataaa
     7021 ctggtaatta agcgttcata ccgtttaatt tttgagctaa tttagctact tttctagctg
     7081 cagcattctt atgtatagtt cctttagaag ctacttggta tattcttttt tgagcgtatt
     7141 ggaacttagc aacagcatcc tctctgtttc cagctgcaac agcatcttcg aatcttctta
     7201 ttgcagtttt tatttgagat tttctaactc tatttaaagc agttttcttt tcgataacac
     7261 ttattctttt cttagctgat tttatatttg ccaccgtgtt cacctccctg tattttttta
     7321 tcatattcat cgtcaacata ttagatttta acagaaataa atctaaaaat caaggattat
     7381 tatttatata ttttaataat gttttttatt ttgatttttt agactgtaga ttgtgtataa
     7441 ataatttgca atatcttgtt tttaggttgg tatattttgt gttttaaata ttttgacata
     7501 tataaattaa tattttattt ttgtaacaaa tattttgaga taatacaaat tattaacata
     7561 cattttgata gtatttccaa taattataag ataatacaag aaattactca tttttgtgaa
     7621 tttaataaaa aattataaaa atacagagga tacagcagat aagttgatat catatataga
     7681 tgaaaattgc agtggtcatc ttttcgcttt gtgtggggta tcacttggtg gtcaaattgt
     7741 catggaactt ttatctcgaa aacctaatat tactaaaaaa gcaattattg atggtagtat
     7801 ttgctaccca agacctctta tggcaaggtt ttgtattact ggtattcgat tttttggagg
     7861 acttttattt agtaagaagg cttgtcgctt tcaaattgct atgatgccaa aattgttacc
     7921 tgaaaatatg caatatccag atgaaataaa agcatattat atgacagata tgccacatgt
     7981 aagaaaagaa acattctata atatgtatcg tacttacatg atgaactaca tacttaaaga
     8041 aaatgttaaa aaaaccacag cacaagtaat gtactggtat ggagaaaaag aaatgaagtg
     8101 cgttaaaaaa tctgcaaaaa tgtttcagtc ttatgtaccc tcttgtgaga tttatgaagc
     8161 taagggctat aatcatgggt atttatcgct ttaccttcca aatgaatggt tggaaattgc
     8221 ggaaccattt tttcaaaagg aataaaggtg ttaggtgatt attgtataat tattgctgtt
     8281 tttttatata gtaaataaat taggtatgat ataaaaaaat tattttggtc aatatattag
     8341 tgaaagctta ataggaaaat tataaataga ttaaaggtag ggcctatata aatagtttct
     8401 acctttttct gtttgtaaat aaatgataga taagaaagtt tactgacaaa tcactgacca
     8461 aacccaattt tcttacattg aaagccttaa ttttacttgt tttaaataaa tttacaattt
     8521 cttgttataa aatactaaat tttgttaaca attataatta gcatttaatt tatactggag
     8581 gtttatatga ttaatgtaag aacagattta gctttagaag ctagtgaaat gtgtgaaaag
     8641 tctcaagaag gaagtagcat tcctggtgta aaaatagaaa ctaaagaatt agaaaattgt
     8701 attgtaacta aggttgaagt aatagacgaa caaggttcag aaataatgaa taaagatata
     8761 ggaaaatata taactctgga aagtaatctt atgaagtttg atgatgatga atcaagagaa
     8821 gaaatgataa gttatctaaa agatgaactg gtagacatat ttggacaaga cgaaaataaa
     8881 aaaacgttag taatcggtct tggaaataga aatattactt ctgatgcact aggacccaaa
     8941 agtgtatcta aaacacttgt aactagacat ctttttaaaa attataataa agattatgat
     9001 gatgatttta ctgaggtatc agctttaagc ccaggagtta tgggagttac tggaatagag
     9061 actagtgaaa tagtaaagtc attggttgaa aaagtaaaac cagatagagt agtagcaata
     9121 gatgcattgg cttcgagaaa aatggaaaga gtaaactcta ctatacagat atcaacagca
     9181 ggaatatcgc ctggtggagg tgttggcaat acaagaaaat ctctaactaa agaaacttta
     9241 ggtgtggatg ttattgcaat aggagtgcca acagtggttg atgcagcaac tcttacaata
     9301 gatgttttgg acatggcaat agacaattta atagctcagt cagaagaaac agaaagtttt
     9361 tacgaaatgt tgaaaaaact taaagaagaa gaaaagtatc atctaataaa agattcatta
     9421 gacccatatg acaagaactt gatagtaaca ccgaaggata tagatgatac aatcgaaaat
     9481 ctatctataa taataagtga aggtctaaat aggtctttac atccaggtag attggtataa
     9541 aataaggagg agcaattatg tttaaaaaat gcattaaagt agtaaccttg acttttatat
     9601 tagcatgcat tttaccagga aaatcattag ctttgaatca ggatgatttt ttaaagtttt
     9661 tagtaaattc atcttatcca gaagctaaag tcgaaggaaa tgatacagaa gataaaaaga
     9721 ataataaaaa taaagaaaca agcaaagaaa ataaggaaga aagtaaagaa gaaaaaacta
     9781 aatctaaaga tgcaagtaaa gttgataata aaaaagagag tgaaaaagaa tatataaaac
     9841 tttatgttgg taaagaaaat gttccagata ttgagtcaaa aaattcagac acaactgaaa
     9901 ctaacgcaac atctagttca gattataagg atgacctaag agtaactaaa gaaaatccaa
     9961 gaattttgat atatcatact catggatgtg aaacttattc aaattcacca gatggaaact
    10021 accattcaag agataagaaa aattcagtaa tggaagtagg aagtgcatta actagtgctt
    10081 tagatagcaa gggatggggt gtagttcata caactaagta ccatgactat ccatcttata
    10141 ataactctta tgcaagtagc ctaaaaacaa tacaaagtat attacctaaa tataattcag
    10201 tggacatagc gatagactta catagagatg ctagggattt aactaatcca gccactaagg
    10261 aaaaagacca tttaaaatat acaactatga taaatggaga aagggtatca aaattcttct
    10321 ttgttgtagg aggtaaaaat acaaatagaa aacaacttag agctttagca gaagacataa
    10381 ctgcttttgc tgagaagaag tatcctggtt tggtatctcc aatagtagaa aaagactatg
    10441 ctagattcaa tcagtttgca gttaagaatc atatgttagt agaaataggg aataacgcaa
    10501 caagcgtaga agaatctaag gctactacaa agtatctagc tgaaattttg gatgaatatt
    10561 ttaaacaaaa aaattagagt tttaaggtat aattaggagc atgttaaatg agccgattag
    10621 aaaaaaatat agaaagtaaa agtaaattaa gaaaatacaa gtatattatg agaattatgt
    10681 ttttgattac tatgattata tgcacatcag tttgtgtatt tatagtagat agtaatgcaa
    10741 aaagtatgct aggaatgaaa caatatgatt ttcccaatat taaatttgat ataacaaaat
    10801 ttgattttga tataccgaac tttgatatac atgaaatcat aagaaaattt aataagtagt
    10861 aataaatgaa taaggtttat tgatgaaaat aagccttatt ttagttatat atctttaatg
    10921 tataaaataa aaattcacta actgtatttt ataggattta ctttaagttt atgatattat
    10981 ataatattta gtgttagact tgataatcca catataatgt ggataatttt gtttagtgcc
    11041 aattagttta tattcaaaaa ttttaaattt ggtatataaa gagtttttag ataatgtaaa
    11101 ataataacta taagaataat caagaggagg gaactttaag gtggacaata aacaaagtag
    11161 aactagaaat tttagtataa ttgcacatat agaccatgga aagtctacct tagctgatag
    11221 attaatacaa caaactgggc ttgttagtga aagagacatg aaaagtcaat tacttgacaa
    11281 tatggacctt gaaagagaaa gaggaatcac tataaagctt caaaacataa gattaatgta
    11341 taaagcgaag gatggaaacg aatattattt aaatcttata gatacacctg gacatgtaga
    11401 ctttaactat gaggtatcaa gaagtttggc tgcatgtgag ggagctttat tagtagttga
    11461 tgcagctcaa ggtgttgagg cacaaacttt agctaatgtt tatcttgcta tagaccaaga
    11521 tttagaaata ttaccaataa taaataaaat agatttacca agtgcaagac cagaggaagt
    11581 aaaaaatgaa atagaagatt taatagggct tgactcaagt gaagcacctc ttatctcagc
    11641 taagactggt ttaaacatag aggatgtttt agaagacatt gtaaaaaatg ttcctccacc
    11701 taaaggagat aatgaagctc cattaaaggc attaatattc gattcttact atgatgctta
    11761 caaaggtgtt gtagcctatg ttagagtatt tgaaggaact gttaaaaagg gtatgacaat
    11821 aaaaatgatg aataccaata aaaagtttga ggtaactgaa gttggtgtta tggctccagg
    11881 tcaaactgaa cttagtgaat tatcagcagg tgatgttggt tatattgcag ctagtataaa
    11941 agatataaga agttgtcgtg ttggtgatac aataacagat tcaaataatc ctacagaaga
    12001 acctttacca ggatacaaaa aagctactcc aatggtatat tgtggtatat atccaggaga
    12061 aggagaaaaa tatgaaaatg ttagagatgc tcttgaaaag cttcaagtaa atgatgctgc
    12121 acttgaatat gaagctgaga cttcagccgc actaggattt ggttttagat gtggtttctt
    12181 aggtctttta catatggaga taatgcaaga aagacttgag agagaattta atcttgatat
    12241 aattactaca gcaccatctg ttatatatag agttacaaaa atggatggag aagtcgtaat
    12301 gatacaaaat cctgcaaact tgccagaacc atctgagata aagatgatag aagagcctat
    12361 agtaaaagga gatataatag ttcctaaaga ctatgtaggt gttgttatgg aactttgtca
    12421 ggaaagacgt ggaaatatgt tgaacatgga gtatatagat gagagaagag taatgcttca
    12481 ctatgacctt cctctcaatg aagttgttta cgatttcttt gatgccttaa aatcaagaac
    12541 tagaggttat ggttctcttg attatgaggt taaaggttat gtagcatcta cacttgtaaa
    12601 actcgatata ttaattaata aagaacaagt ggatgcactt agttttattg tccatgaaac
    12661 acgtgctttc cctagaggaa aggcaatgtg tgagaaatta aaaggggaaa ttccaagaca
    12721 tcagtttgct ataccaatac aagctgctgt tggtaataag gttattgcaa gagaaacaat
    12781 aagtgctcta agaaaagatg tccttgctaa atgttatggt ggggatatat ctcgtaagaa
    12841 gaaacttctt gaaaaacaaa aagaaggtaa gaagcgtatg agacaaattg gttctgttga
    12901 agttcctcaa aaagcattta tgtctgtgct taaattagat gaataaatcc ttataaattt
    12961 agataaagat atttaaaagc tagagtgatt ttagaattat ttctaaatta gtctagcttt
    13021 tttatggtat attttaagta aactcataaa gaatataagt aagaatatta aagaagaata
    13081 aaaaggtgat atttatgatt aatatagaaa ttcctaaaaa agtagattat ataattaaag
    13141 aattagaaaa gaatggttat gaagcttata tagttggagg atgtgttaga gattgtttat
    13201 tagaaagagt tccaaacgat tgggatataa caactagtgc tagaccagaa gtggtagtag
    13261 aattatttga aaaaacaatt ccaactggta tacaacatgg aactgtaaca gttatggttg
    13321 aacatgaacc atttgaagtg acaacttata gaattgatgg aaattatagt gatggaagac
    13381 atccagattc aatagaattt acaaataaca tagttaagga cttatcaaga agagatttta
    13441 ccatcaattc aattgcatac aattcagaaa caggattagt cgacccattt aatggatatg
    13501 aagatataca aaataaatat atacgttgtg ttggaaatcc agtagatagg tttgaagaag
    13561 atgcgcttag aatgcttaga gcagttagat tttcagcaca acttaatttt aatatagatg
    13621 aaggtactga gcaaagtatt cataaaaaat cagatttgat aaaaaatgtt tctattgaaa
    13681 gaatccagac agagtttaat aaaatcttag tatctgattc atctaaatta aacctattaa
    13741 aatcaacagg tttgttgaaa tttataattc ctgaaatatg tgaattggaa gatgtcactc
    13801 aacacaatcc atatcatatc tatgatgtac aaaaacatac tttaatagct acagaagtaa
    13861 ttgaagatga actgtattta aaacttacaa tgttgtttca tgatttaggt aaaaaagtaa
    13921 ctaaaacaac tgataaaaat ggtgttgacc atttttatac tcatagtaga gaatctgtaa
    13981 agattgcaaa gaagattttg aaaagactta agtatgataa ttatactatt aataaagttt
    14041 tgatattgat acaatatcat gattatagaa tagaacctaa aaggaagata attaaaaagt
    14101 tattaaataa acttgaagat gttgaattat ttgaagattt aataaaagtt aattgggctg
    14161 acacacttgc caaaaatcca aaatatgcaa aacaaaagat attaaatctt attgagtgtg
    14221 aaaaagaatt taaacatata ataaatcaaa aggagtgctt taatcttaaa gatttagcta
    14281 ttaatggaaa agatttgata tctataggta taaaaccagg taaagacata ggacatattt
    14341 taaataatat gcttgaaata gttataaata atccagagct aaatgaaaaa gaaatactaa
    14401 aagaaaaggc attaaatata tatacatttt agattcaaat ataaaaaatt taatagattt
    14461 aagaaatata gaagaaaata tgtatttaga taaaataaat aatttttaga gaatatttta
    14521 gagattacga ttgtatgagt tttaaatgca tagggattta ctctaggatt atagactaat
    14581 ataaaaaaaa actttgggaa tactaaaacc ataaatttat aaaattaata ttttatatac
    14641 tgtaaaacag tgaggaggaa cagtatgcat actcaactac aaaagactat tggtctttca
    14701 gcagctcttt ctactgttgt aggtatggtt ataggttcgg gagttttttt taagccacaa
    14761 gcaatataca ctacaactaa tggagctcca gggctaggta ttatagcatg gcttttaggt
    14821 ggttttataa caataactgc tgggcttaca gctacagaaa tttcagctgc tataccgaaa
    14881 actggtggta tgatgattta tatcgaagaa atatatggag aaaagttagg ttttttaact
    14941 ggatggatgc aaactgtact atttttccca ggtacatcag cagcactagg agtaatattt
    15001 gctcaacaag catctgaact tttaggaatg agcccaaata atatggcaaa tgtacttcct
    15061 atagcgattg gagttatact atttttagca ttattaaata taataggttc atctttagga
    15121 ggaaaagttc agacagtagc taccattgga aagatgatac ctttaatttt aataataatc
    15181 tttggattta ttaaaggtca aagtagtgaa gtgttaaatc catttgtagg agatggtgta
    15241 aatgcatcaa atgcacttgg acaagtatta atagctactt tgtttgcata tgatggttgg
    15301 ataaatgttg gagctatatc aggagaaatg aagtctccag agaaagactt accaagagca
    15361 atagttggtg gtttatcatt agttatggca gtttatatta taataaatat agcttatctt
    15421 tgggtagtac cagcaagtga attagcaaca gttacttcac cagctacatt agtagcaaca
    15481 agattgtttg gaaatatagg tggaaaagta ataactatgg gtatattaat atcagtattt
    15541 ggcacattga atggatattt attaacaggt tcaaggatac cttatacatt agctgaaatg
    15601 ggaactttac cagcatctaa agctctttta aaagtaaatt ctggaggttc tcctgtaaat
    15661 tcaatactac ttataactgc gttagcatgt gtatatgcat tatctggtca atttaatctt
    15721 ttgacagatt taactatatt ttcaatatgg gtattttatg tattaacttt tataggtgtc
    15781 atgaggctta gaagacaaaa gccagattta tacagaccat atagagtccc tttatatcca
    15841 ataataccga ttatagctat attaggcggg ctgtttgtaa taataaatca aatattaact
    15901 tcaacagtta tatctctagg cggaatattt ataacactat taggtttacc agtttattat
    15961 tatatgaaaa aaagataact acttaattta agttagatga atttgggtaa aagtaaaaat
    16021 taacttttat ccaaattttt tatactatta cacaatataa gtattatata ttacttgatg
    16081 tgctattttg tttgatttat aatttagtaa ttgatgtttt aagataattt aagaaatgag
    16141 gaataaaaaa tgctaggact ttatgtacat attccatttt gtgtgaaaaa atgtaagtat
    16201 tgtgacttta attcttataa aatggacata gatttaaaaa aaagatatat agaagattta
    16261 aaaatagaaa tggaattata tagtaataaa ttatataaag ataataaata taaaaataaa
    16321 gaatgttgta gtctaaataa aaatgacaaa ataacgagta tatttgtagg gggaggtaca
    16381 cctagtatac taacatctga tgaaataaga gaagtattta tgagtataaa agaaatgttt
    16441 tacatagatg ataatgcaga aataactata gagtgtaatc ctggtacatt aacattagaa
    16501 aaattaaaaa ctatgaaaga tattggtata aatagactta gtatagggct acaagctatt
    16561 caagaaaagc atttgaattt tataggaaga atacatactt atgaagaatt tgagaaaaac
    16621 tataaagatg cattaagtgt aggatttaaa aatattaata tagatttaat gtacagtctt
    16681 ccaaatcaaa ctctatgtga ttggaaggaa actcttgaaa aagttgtaca cttaaatcca
    16741 acacatatat cagcttattc tcttatacta gaagaaggca ctgaactata caatatgtat
    16801 gaaagtaata aatttgaact tatagatgaa aatatagata tagaaatgta tgaatataca
    16861 ataaactacc taatgtcaaa aggatataat caatatgaaa tatcaaatta ctcaaaagaa
    16921 ggctttaatt gtaagcataa tattttatat tgggaatgta aaccgtatat agggattgga
    16981 gcaggagctt ctggctatat taatgagaac agatacaata atgtagaatc tttagaagac
    17041 tatcatttaa gtttggtaaa aagggaaaaa ccaatacaag aaaatgaaat tctttctgag
    17101 aaagatatga tagaagaaaa gatatttatg gggcttagaa tgaataaagg tataaaattt
    17161 gaagatttca aaaaaaaatt tggaatagat tttaaagata agtatagtaa gcaaatagaa
    17221 atgctatcag ctagaaagct tataaatcag agctttgaag gtatacaatt aactcaaaaa
    17281 ggaagagaaa tctctaatag tgtatttata gaatttatgg aataattatt aaggtgttga
    17341 caaaataaaa ttataatggt atataatata tataatcttt ttagcactcg aacttagtga
    17401 gtgctaacag ctaggaggta taataatatg gaattaaatg aaagaaaatt aaatatcctc
    17461 aaagctatag ttaaagatta tatagaaaca gctgaagcta taggttctag aacaatatct
    17521 aaaagacatg atttaggtgt tagtgctgct accataagaa atgaaatggc tgaccttgaa
    17581 gaattagggt atttaattca gcctcatact tctgcgggca gagttccatc tgaaaaaggc
    17641 tataagttat atgtaaactc tcttatgagt aaaagcgagt tggatgataa tgataagatt
    17701 cttattgaac aatgtatgaa tcataatata aatcatataa aagaattgat tcatgaaact
    17761 tctaagctat tatctcaact aacaaactat acaactgtag cagttactaa gagtttagta
    17821 aatcaaagtg ttataaagca catacagttg gttgctatga atgataataa tattttactt
    17881 atagttgtaa cagataaagg ggacttgaaa aaagctaacc ttacaactaa tgtatattta
    17941 gatcaatcta agttaaattt gatttctgat aatcttacaa ggaagctctt aggtaaaagt
    18001 ataacagatt tggatgataa tctaattgct tttattaaat acgaaataag tgagtattct
    18061 gggcttatag atgagctttt aaatgctttg aactcaaata tgaaagaaga agatttttct
    18121 ctatcattaa atggagctac taacatattt agttatccag aatttaatga tgtattaaaa
    18181 gcaaagtcat ttttaaatat gttggagaaa aaggaaacaa tagctgacat aattaaatca
    18241 aagggaatac aaaaagacaa tctaaatata ataattggta gtgataatga ttgtgaactg
    18301 gcacaggatt gtagtatagt tacagcaact tataatgtag atagagactt ggttggacga
    18361 ataagcttca taggtcctac cagaatggat tatgctagaa tttactctat aataaattat
    18421 atgagtttat taattaatag aaagtaaaga aattttaaat tgcgaggtgt aaagtcttgg
    18481 ataaaaaaaa tgagcaacag gaagtaagag aagaaaatga tacttctatt aaccaagaat
    18541 cggaaactca agtagagttg gaagaggaag ttgttaatga agaatgtgaa acatcaaatg
    18601 aaaaaactga tgaaaaagaa gttgatgatg aaaatgtaac agatataaat tctaagttag
    18661 cagaaaaaaa attgcagaat gagttggatg aattaaatga taaatatcaa agacttcaag
    18721 ccgaatatgc aaattacaga agaagaactc aacaagagaa agagacaata ggtgtatttg
    18781 caaatgaaaa aataataact gagttgatac cagttataga tagtatggaa agagccttag
    18841 atgcatgtga agataaggaa gatactatgt ataagggtat aagccttgta cataaacaat
    18901 taatagacac tttagtaaaa tttggagtag aagaaataga agcagaatct aaggaatttg
    18961 accctaattt acatctagca gttatgcaag aaagtgtaga tggaattgaa gcaaaccaaa
    19021 tagtaatggt acttcaaaaa ggatataagc taggaactaa agttgtaaga ccttcaatgg
    19081 ttaaagtttc ttgctaagta taaattaaag gttacattat ttaacctaat ataaaatatt
    19141 aaaataagta aaacttaatt atataattac tcgaggagga ttttagtcat gggaaaaata
    19201 ataggaatag atttaggaac aacaaattca tgtgttgcag tgttagaagg tggagaagca
    19261 caaataatag caaatagtga aggtatgaga actactccat cagtagtagc atttacaaaa
    19321 gatggtgaaa gaatagttgg agaacctgca aaaagacaag cagttacaaa tgcagataaa
    19381 acaataactt ctataaaaac tcatatggga actgattata aagtaaatat agatggaaaa
    19441 tcatatactc cacaagaaat atcagcaata atattacaaa aattaaaatc agatgcagaa
    19501 agttacttag gacaaacagt aacagaagca gttataacag ttccagctta ctttacagat
    19561 gctcaaagac aagcaactaa agatgctggt agaatagcag ggcttgatgt taagagaata
    19621 ataaatgagc caacagcagc agcacttgct tatggtatgg ataaattaga ccaagaaaag
    19681 aaaatattag tatttgactt aggtggagga acttttgacg tatctatact tgaaatagga
    19741 gatggaactt ttgaagtttt agctacagct ggtaacaata gattaggtgg agatgacttt
    19801 gaccaaatag taatagatta tttagcagag gaatttaaaa aagctgaagg tgtagattta
    19861 agaaatgata aaatggctct tcaaagatta aaagaagcag cagaaaaggc taagaaagaa
    19921 ttatcatcaa caatgagttc aaacataaac ttacctttca taactgctac agcagaagga
    19981 ccaaaacact taaatataga cttatcaaga gctaaatttg aagaattaac tagaggttta
    20041 gttgaaaaaa ctatggaacc aactaagaca gctttacaag atgcaggact ttctacaggt
    20101 gatatagatg atgtattatt agtaggtggt tctacaagaa taccagcagt acaggaagct
    20161 gttaagaagt ttataggaaa agagcctcac aaaggtataa acccagatga atgtgttgca
    20221 gcaggtgctt ctatacaagc tggagtttta gcaggggatg ttaaagactt attattacta
    20281 gatgttactc cattatctct tgggatagaa actatgggta atgtaatgac taagataata
    20341 gaaagaaata ctacaatacc aactaagaag tctcaaatat tctcaactgc tgctgataat
    20401 caaacagctg ttgatataca cgttcttcaa ggtgaaagaa gtatggctta tgacaataca
    20461 actttaggaa gattccaatt aacagatata ccaccagcac aaagaggaat acctcaaata
    20521 gaagttactt ttgatataga tgcaaatggt atagttaatg tttcagctaa agatttaggt
    20581 acaggaaaag agcaaaaaat aactataaca tcaaacacta atttatcaga agcagaaata
    20641 gagcaaaaaa taaaagaagc agaaatgaat gcagaagctg acaaacaaaa gaaagaaaaa
    20701 atagaagcat tcaatcaagc tgaatctact atatatcaaa ctgaaaaaac tttaaatgaa
    20761 cttggagata aaataagttc aagtgaaaaa gaggagatag aaaaagctat agcagactta
    20821 aaaactgtaa aagataatca agatgctaca gcagaagaac ttaagaaagc tacagatgaa
    20881 gtaatgacta agttccaaaa agtatctcaa gagatgtacc aaaaagcagc tcaagagcaa
    20941 caagcagcac aaggtgcaga acaagctcaa gacaatggac ctaaagatga caatgtagta
    21001 gatgctgact ttaaagaagt ggatgaagat aaataagcta tataatatgt aacactaaat
    21061 gatagttaga gcatatatga tttgtgaaaa tcatatagat gttaaagata tatggacaat
    21121 acacatcata aagaatataa tatatcatag attatataac ttaaagtaga atataagaat
    21181 taatgatagc ttgtagttta ttattctaca agctatcgtt ttgtaaaatg aaggtggtgt
    21241 cagtttttga gtacaaaaag ggattattat gaagtcttag gtatcagcaa aggtgcagaa
    21301 gctcaagaga taaaaaaagc ttacagaaaa ttagcaatga agtaccatcc agatagaaat
    21361 cctggagata aagaagcaga ggaaaaattt aaagagataa atgaagctta tgaagtctta
    21421 tctgatgata caaagagaaa aacttatgac cagtttggac atgatgggct aaatggtcaa
    21481 ggtggttttg gtggtcaagg aggatttggc ggtcaaggtt ttggtggttt tgaagatatg
    21541 ttcggagata tatttggtga catgtttgga ggaagcttcg gaggaggaag aacaagaaga
    21601 agaggacctc aacgtggtgc agatataaga caaagtgtta ctatatattt tgaagaagct
    21661 gcatttggta aaaagatgtc tataaaagta aatagaagtg aagagtgtga agaatgtaat
    21721 ggaactggag caaaaccagg aacatcaaag aaaacttgtt caacttgtaa tggaacaggt
    21781 caagttagaa ctgttcaaag aactcctttt ggtaatatag caagttcaag accatgtagt
    21841 acttgtaatg ggactggaga agtgattgaa tctccatgta gtaaatgcca tggaactgga
    21901 aatacaagaa aagttaagac tatagaagta gatatacctg ctggtataga tgatggtcaa
    21961 atgataaaac tttctggtca aggtgaagta ggagaaaaag gtgctccaag aggagactta
    22021 tatattgtag ttaatgttaa gtcacatcca ctatttacta gagatggaaa tgatatttat
    22081 tttgaaatgc caattacatt tgttcaagca actttaggtg atgagataga agtacctact
    22141 ttggatggaa aagttaaata tagtgtacca gaggggacac aaactggaac tgtatttaga
    22201 cttaaagaaa aaggaatccc tagaattaga ggtaattcaa gaggagacca atatgttaag
    22261 gttgtagttg aaattcctaa aaaattaaac gataagcaaa aagaactatt gagagaattt
    22321 gcgaaggagt gtgggtcgaa cgtccacgaa aagaagaaaa ccttcggaca aaagatagaa
    22381 gatatgttta agaaaaagta gttaaagtag ccaatatata atcatatttt tgaaatctta
    22441 gttatttata agattgtcaa aggagatatt atgattttta tattggctat ttttgtataa
    22501 aaaataaata tgcataaaat aaaactgaat ataaataatt ttctgaaaaa tacttgtcct
    22561 aaaatatata atatgctata atataatcaa ataaaagaaa ggggtgattc cagtgggaac
    22621 ggatgtggca ctgccagatg acgaagaaaa ctttattgta atctacaaaa taaaattaga
    22681 tgcttttagt gctagattaa gtactggaaa aactcatgag ctgggataat tattataatt
    22741 acctagactt aaatttttag tagtaaaata gtaatattat atagacaagg tatctaattt
    22801 ttaaatcttt tgtatgaatt aaaattttta taaaggatat atctgattag aatgtaatta
    22861 gggaaaccta gttgtaaatt gcttttagta tatacatgtg taaagaaata tgtatatggt
    22921 attggttatt gtgctagatg catatgtaaa ggaatatgta tttagttgag tatatgtaaa
    22981 gaaatatact tataatctat gaaaaaattt tagtataaat tgctaaaaca ttgagattgt
    23041 tttttataga aagttagttt ataccaatta aaaatgtgct taagatttgt ttgatgttta
    23101 attataaata agattgtaga taaaaaaata agtagagaca aaggtttaaa atacttgatt
    23161 taaatatagt ttgttttgaa cacttgccaa attaaccaaa ttaaaataat taataaaata
    23221 gacatcttct catagtaaaa tatactgtga gaggatgttt ttgctttgtt cataaacttt
    23281 tatgaaaaat ttgcatagtg tcacatttta atatactata tatataataa aatatagata
    23341 aaattaaatg attcaaaaga gaaaggtgaa aagagatgta ttatataggt gtagacatag
    23401 gaggaacagg tatacaagca ggagtagttg ataactatgg gaagataatt tttaggtcag
    23461 agtgtaaaac agtaattgaa aaaggctttg aaggaatact taatgacata aaaataatga
    23521 tttataagtt gttggaagat aataaactta ctatgagtga cataaaatct attggatttg
    23581 gtgttccagg ttttataaat aaagaaggat tagtaacttg tgtaaattta aaatggaata
    23641 aaaaagcttt taataaagag ttaaaaagaa gatttccaga tgttgaaatt catggagaaa
    23701 atgatgcaac agtagcagca cttggagaag caaagtttgg aagtatgaaa ggtgctaatg
    23761 ttggagtatt atatacttta ggaactggtg taggtggagg aatagtaatt aatcaaaagg
    23821 tcttttctgg agcacatgga ttgggttcag aaataggtca tcaaattata ggagaaaact
    23881 attttaattg taattgtgga aataatggtt gtgtagaaac tttttgttca gcaactgcaa
    23941 ttataaagta ttctcaaaag ctgatagaag aaggagaaaa gagtaggatt ttggatttgg
    24001 cagagggcaa tttagaaaat gtaaatgcaa agatggtgtt tgatgcatat agagaaaatg
    24061 atttagtggc aattaaagta ataaatagat ttaaagaata tttggcaaaa acttttgcaa
    24121 atacaataaa ttctttagac ccagaaatta tatccatagg aggaggtatt tctaaatcta
    24181 gcgatataat acttgatggg atagaagatt tagtaagaaa atttgtatta tacaaaacag
    24241 aagatatagc tacaattaca tgtgcaactt taggttcaga cgcaggaatt ataggagcag
    24301 cttttttata aagtaaatga actgcttatt ggaggcggga tatatgagta aaacaaatgc
    24361 tagaagaacg ctttttttga tagccatagg ttcaggaact atgctgaatc ctcttaattc
    24421 ttctatgatt tcacttgctt tacatagtat acaaaatgat tttcatatta gttttacaac
    24481 tgtatcttgg attgtttcag cattttacct agctagtgct attgcacaac cagtttcagg
    24541 taaaatagga gatttaatag gtaggaaggt cttatttttg agtggtttga tgcttgtaat
    24601 tttatcagca tccatagtgc cacttgttca atcattcttt atattgattt ttataagagt
    24661 aattcaggca attggaacga gtacactata tccatctgga gttgctctta tacaaaataa
    24721 tataaaagaa agacagtctt ctgcattagc agttctaacc atttttgctt caactacagc
    24781 aggattaggt ccaacgcttg gaggattatt gttggattta ggaggatggc atgctatatt
    24841 tcttgtaaat atacctgttg ttttggtttc tatatgctta gggtattttt tattcccaaa
    24901 agaagtaaaa gaaaagaaat ctataaaaga aactctgaaa aatgttttgt caagattaga
    24961 tattattggc atacttctat tttcaattgc aatgattttt atattattat ttttgctatc
    25021 tatgaaggag tctttcaact tagaacaact tgtatttggt ataatactga tgtatctttt
    25081 tatttggcat gaattgagaa caaaattacc atttatagat attaagcttt ttgtgagtaa
    25141 ccctaaattg tcaaaagttt acttgcaatt tattatatta aatttcttta attatatttt
    25201 gttttttggt cttcctagtt attttcaaga tgcattacac tatagtgcaa aatcgactgg
    25261 attatttatg ctatttatgt ctggtatagg tatatttata tctccattaa ctggaagatg
    25321 gatagataaa tctggaacta gatttcctct tgttacatca tcaatcttta tgtttattag
    25381 tgcagtactt ctttcagtag tttttattca tataccagta ataggaaaag gtatcatatt
    25441 atcattagca ggtattagtt atggagttgg taatgtagcc ctgcaatcct ctatgtttga
    25501 agaaagtcct cgtgacagta tagggacagc ttctggcctt tttcaaacta gtcgttattt
    25561 aggttctatt ttagcaactg ttttacttgg aatggtattt gaagtaaata taacatcaga
    25621 acaatttcag atattaggtt atgtgatggt ggtcttaggt acaataagtt ttttattaaa
    25681 ttttatacta aagaaagaat taagaaaagt agaatagtgg atttatggat ttaataagtc
    25741 taataactga taagtttaaa aacattgttt gtaaaatata actagtttga tatttattaa
    25801 ttctaagctt tgaagcatag agtactcgat aagtatacaa tattttgaat taaaattaag
    25861 cagaaatttg aactagtctt tcaaatctct gcttaattta gtggttaaat ttttttatag
    25921 agaaaataag atttgacact cagataaaaa atcattgcat tttcaaaatt ctttaaatca
    25981 aacccaagta tctcattaat cttttccaat ctatatacta gcgtgtttct atgtaaaaac
    26041 atactagttg atgtcttaga aatatttaaa tcattattta taaattttaa tcctgtatat
    26101 agaagttctt catcaataga tttaatctta ttaaaatcta ttaatttatt gagtatattg
    26161 tcctcattaa ttaaatctaa ggttttatat ctaataaggt caaacaaatt taaaaactta
    26221 ttttcagcgt tgtcttttat aatatgaggt agtaaatcag aacataactg taatctaaaa
    26281 tcaaaatcag ctttactatc taatttacca agataaaaaa tataagtact ttgataagtt
    26341 tcagtttcaa ttatattttg tgtagttaca ttaatatcta agttatattt agaatttact
    26401 ataaaaa
//
LOCUS       DAFNNB010000036        26021 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0036, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000036 DAFNNB010000000
VERSION     DAFNNB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 26021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26021
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0036"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            197..907
                     /locus_tag="L3M60_001363"
     CDS             197..907
                     /locus_tag="L3M60_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226042.1"
                     /translation="MIKIAICEDEKEQQELLKRYINQIFDALSVKCRLEVFNSGEELL
                     EGYSKDTDVLLLDIQMGQINGIDTARKVRVLDDKVEIIFITALIEYALEGYEVRAYRY
                     LIKPVKYNNLKDNIINCIKEVDIKNKYIMVKAQGSQTKLNVNEITYIEVQKETITIHT
                     LNEVYKINGTMSNIEKEIDCSRFFRCHKSFLVNLEHVKSIKQYVAILENSKEVPVSRY
                     RFKETKDKFFDLIEEKLC"
     gene            901..2238
                     /locus_tag="L3M60_001364"
     CDS             901..2238
                     /locus_tag="L3M60_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6226043.1"
                     /translation="MLDSVYSLAIINMVIFIALFFMLMNKIGYTYRCKTNIQLILILI
                     LSAILIINGMPFGIVPLHALIRIILILSLICKITYQINIKKATINSVIYSLLYILIGC
                     SMYWILQFSWKYVGHYILKANTVINFIIIFIGVYFFDILQKIYNNKKYYLYIILTIAV
                     NVVIVLFVNISTEKMDDLYTIVTKNHIEYSYNVYTMYFSLFMKNTFPYILFIVNVLLI
                     SIFINSIKSEKEKIKAEFVSEKLDMQYKYYFMVKESQEKMRQVYHDMNNHMKNIKSLK
                     NSSEDVNKYINSIEDEVKNTKNIYNTGNVLLDIILYEKSRLCMENNIDFNVGIDFSKC
                     EFIEMIDVSSIFSNLIDNAIEACSNIDDNNIEKYITIKSTFIKGYYIIRCENSKMNKV
                     IIKNNKIFTSKKDKFLHGIGIESIKSSIRKYNGELKIKDSEYKFIVTAHIPIE"
     gene            2415..4982
                     /locus_tag="L3M60_001365"
     CDS             2415..4982
                     /locus_tag="L3M60_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226044.1"
                     /translation="MTTFKLAVSYMKRQKGKTVALLSCIVLAVMLIFSMIVIRDSGYD
                     SQIKEAKDLHGDYHIWFEGLEKDKTQDLTNEEGISKSNTSKELCEVVDKESGVKLYLN
                     SFDKGFLSSLGYKIEGREPIKDGEIVIEKEATRQMGISNPLNKNIDLMLLNKYIDDNE
                     INHMDSANKTFKVVGVIEKLDKYYDKSRYSITLRAFVYKDSNLPIKIKDTYNGTIYLK
                     SEKNIPQFITKMTKKIDTNVFNLHENGEVDLAKNQRQNSKFSKENIINSVLLVSVSTI
                     VIYNIFNIIFQDMTSQIGIMKSIGMSNKKVRNMLIIMSFIYIILGTLVGIVFGMIFSY
                     VGLRVVYGYSSMLTIQISSVICSFAVSIISVFISSFIVIKKFRKMSIIEAIRSSDKYE
                     KKSKNDNKKIDGKSKNLLISMATRNLWRNKPRTILTILAITLVGIMFILNLGAKSLLK
                     KNMEEGITGGSWSMSYGSVDKTVEGDSRSSESLFYKLDNNLIQKVNGMKGVRHVEPHF
                     YNHRGHILLSKNKLSKAYKDELDRKNSSYQEEHNNEYPLLIRGYSDDMLKQRQEFIEK
                     GENILSTTSGKYKKVILVNNTNSQVTHSFDAKIIDDVKIGDIIEIKLPVYRDGIEKYE
                     NFKVEVGAIMKELYASSQDGNTQAQGAQMIFKENDYKELTGQKEYNKLFVIVEKGQLY
                     SVERRLEETTKNYGSTEINGKGEELKLIGMQQSSEERLSVIYQFLTILILAVNIIFIM
                     RSNIITRRKELATLRAIGMSIKSIKKILIIESQMYGMVASIIGAIIATVYHNYGLAKT
                     NKVLLEGGYTRIIEPNIPFSQIIILFTIFIAMGFISIYVSKDKIEGTTITEGISQNN"
     gene            5041..5724
                     /locus_tag="L3M60_001366"
     CDS             5041..5724
                     /locus_tag="L3M60_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226045.1"
                     /translation="MENRIMKKLAIKNIKKVYGKGDNKVYALNGIDLEIQPNKFTTIV
                     GQSGSGKSTLLHCMAGLDKPTDGKVFIDDLDLYTLNDDKLSKIRREEFGFIFQSYNLI
                     PVINVYDNIVLPISISKRKIDKDYIIDLIAKLGIKSQIDKFPNELSGGQQQRVAIARA
                     LANKPSIIFADEPTGNLDSKTTNEVVKLLKLCVYEYKQTLVMITHNAEIANNSDNIIN
                     ISDGKIVLI"
     gene            5987..6847
                     /locus_tag="L3M60_001367"
     CDS             5987..6847
                     /locus_tag="L3M60_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6226046.1"
                     /translation="MKIIQILNNNVALVKRGKNEIIVFVKGIGFKKRVGQVINENEIE
                     RTYILDSYDMLEHFSYLLQNSDSNNIVLINEIIEYGEKVLNIKSSDYLSLTLLDHLEF
                     LLKRCEKNQFIKSPLVWNVKRFYPKHFEIGMYALKLIGDSKEINLPDDEAVSIALHFV
                     NMEVNKESHDSTIVELRTLADIVSIIKYHFNVEIDETSTNYMRFTTHLQYFIQRIMSD
                     EASIDEDNSIDLYKQVSQLYPKSFGSVQKIRKYIKSQFNVDISISEETYLMLHINRVT
                     QRLEAKSDEI"
     gene            6837..8708
                     /locus_tag="L3M60_001368"
     CDS             6837..8708
                     /locus_tag="L3M60_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6226047.1"
                     /translation="MKYKDLNENIIKLTGGKENIKSVAHCVTRLRLTLNDRSIAQTEE
                     IKKLKGVIDVVSNDVAYQIIIGTHVVDVYNEFMSMIGLSSSESSVKGERKKGIRGIKG
                     IFNSIFVVVSETMTSVIEVLLAAGILASILALLNMSGILHSDSPTYQILDTLRSATFH
                     FLPVLIAIASAKRLNVNPYIAVVLAVTLLSSSIDGVEGLSLFGINLQATTYANSFIPI
                     LLGVWSMGIIIGGLKKILPKALHYFLVPVLSLIITLSVTLTIFGPIGMWIGDVLNYAC
                     MFLIDTLGNWSVVALYAALQPLLIVFGAANFTMPIALNSVTTLGYDPIFMGAATISDI
                     AVCGAMFGYFLKTRNKEQKQLFGTVSFSALMGVTEPSVFGVFMKYRRPFVAVMIGGGL
                     GGLIAGLAQVKTYGMVWGLAALPTYLVGGEMSNFTFMIIAVLVSFVGATVASYLLGIP
                     SDEKEEAKNANEKELELVVNEDNSRTISVGSVAKGQVISLEEVNDKAFSSGALGKGVG
                     VIPSESKSTVMSPVDGTITIVFQTKHAYGIVTDEGLEILIHIGIDTVNLDGKFFESLV
                     TQNQRVKKGDALAVFDSDKIIEAGFDSTIITVVTNTSDYLDVISSNDESEELLTVIV"
     gene            8728..10170
                     /locus_tag="L3M60_001369"
     CDS             8728..10170
                     /locus_tag="L3M60_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBN6226048.1"
                     /translation="MNIKDTFFWGGSIAAHQCEGSWDSDNKGPAIMDFVTKGSYETPR
                     VITDKIEEKLDYPSHNGIDFYNRYKEDIALFKEMGFSALRISIDWSRIFPNGDDENPN
                     ELGIKYYECLIDTLIENNIEPIVTLYHFELPMNLVHKYGSWNNRKLIDLYLKYSETVI
                     KKFDGKVRHWVTFNEMNHIDPQTEHSDIFTYMIAGIKYSEIENKVQTLANVGYNMTLA
                     GVKVAKLAREINPNNKVGCVFGLNPIYPYNCNPSNVLNAFLDNDRDYYQIDAMCNGKF
                     PQYKLKEYEKNNINIEVTDEDKKAFEEGRLDFIGLNYYFSSVSTPKENLSGDKSLFGG
                     IQNPYLEQSKWGWAIDPVGLRFTLNYIYRKYQLPILITENGLGAYDKIEEDGTINDNY
                     RIEYLSKHLSQMKSAIEEDNVECFGYLMWGPIDLVSATTGQMSKRYGFIYVDLDDEGK
                     GSFKRYKKKSFDWYKEVIKFNGENLNLLDV"
     gene            complement(10449..10982)
                     /locus_tag="L3M60_001370"
     CDS             complement(10449..10982)
                     /locus_tag="L3M60_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBN6226049.1"
                     /translation="MNMKKEALEVLKNRRSIRKFKSNQVSKEELDLVLEAGTYAPTSK
                     GSQEPIIIAVQDEDTVSQLDALNARILNIEKTPHPYYGAPTILLILAPEDALIPVENC
                     SLVAGNLMNAAYAVGLGSCWIHRAKEMFESIEGKELLKKWGLPETLKGVASIALGYPD
                     CPHPKAASRKEGYILRV"
     gene            11120..11557
                     /locus_tag="L3M60_001371"
     CDS             11120..11557
                     /locus_tag="L3M60_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBN6226050.1"
                     /translation="MRMSTQFTVSVQMLLLIMVLDDRKVTSEMISKSTGGNPVMVRQL
                     FGKLKEAGILNVSAGRGATVLAKKPSEISLWDIYMAVEEYSSSELFKFHPKISEGCNI
                     GRFFKEILGNHLDEAIHAMADKMSKVSLAQLLEEWESVNKESK"
     gene            12745..13953
                     /locus_tag="L3M60_001372"
     CDS             12745..13953
                     /locus_tag="L3M60_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226051.1"
                     /translation="MENFWNPLTCFTVCVMIYAFSEFLSKKTKGAISTLLFVCIIFLI
                     GFWTNILPKDITGASGILAAMSNFGIALLITNLGTLINLEDLCHEWKTVVIALIGIVG
                     IGVGCLTVGSMLFGREYALIAAPPIAGSTVAGIIVTTVAEAANRPELAAFVILVLSFQ
                     KFFGIPITTYCINKELKFKRDNGDFLKDESKSNFKLPNMRIFKEDLGKKSSTLVYLAK
                     LSFVAMIGNFVGLATLIPGFTPANYYLNPNIACLLVGLIFTRIGFLEKGILDKSQSNG
                     IIMFGCMLMLPGGLAKVTPTALFGMIVPTIGILVVGSVFIILFTSIVGKLLGYSPRMS
                     AAIGVTCMLAYPATQIISNEGIDAMEGTEEEKQRATDYLLPKMIIGGFVTVTIASVAF
                     ASIIGPIIFG"
     gene            13984..15363
                     /locus_tag="L3M60_001373"
     CDS             13984..15363
                     /locus_tag="L3M60_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226052.1"
                     /translation="MIGSISINEIDKKRFKLDDLSRKIWENPEKAFKEFKACENTANF
                     LRSEGFDVEIGVGGLATAIKASFGSGKPVIGFMGEFDALPGLNQKVSTKQEAFELGAY
                     GHGCGHNLLCTAHVGAVVGIKREMIENNLGGTIVFYACPGEEQLTGKGFMARGGAFEG
                     LDLAINFHPNKISEATIGTSTAVNSVKFHFKGKTAHAGSDPQNGRSALDAVELTNVGA
                     NYLREHVTSDVRIHYTITDGGVAPNIVPDKASVWYYIRALSREAVESTYERLVKVAKG
                     ATMMTETEVEVEFLGGCYNTLNNHTLANLVSECMNEIKQEPWTQEELDFAAELDRQSP
                     QQYKDMISKYNLQEGTHLYYGGGNVTNFNSYGSTDIGDVMHIVPTAYFFTGCTNLGAP
                     GHSWQFTSCAGSSIGEKGMIYAAKIMAMFGAKILNNPEIAKKAKEEFDKSMNGKTYKC
                     PIPDDIPTP"
     gene            complement(15740..17068)
                     /locus_tag="L3M60_001374"
     CDS             complement(15740..17068)
                     /locus_tag="L3M60_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226053.1"
                     /translation="MMKIAVITTEFLKEFVDNSIKKLNINAEIEIYIYRDFSHVGDLY
                     LEIEDSFDGFAVSGPIPKKAITKKAGTIKKPLVDFGTDLQSYYDMFLKLIFKYNTLDF
                     SRAYFDLTDWADNPKDISYYLSNGTFGDLMDSIDENASKMSLSDIAKLEKKIAKKHIN
                     LWNEGKIDFSTTRFSSIVPMLQDAGVNFHFIYPDIDLLKNTFNNLIKDIHLHRMKGNQ
                     PSVIYITVKDTNYDDSTLDQVYKILNSIKKDKLTDFIIEKNSNSFKIFTKCETIKSLT
                     KNFTTCYIKESIENSINIKVCIGYGIGNDIKQAMSSAISANKESKINPLNYSFLVNEN
                     YETIGPLLCDKLLTVSNKVTPHINTIAKSSKLSTLTIQKIISVTEILQRDEVTPKELS
                     SCLNVTVRTANRFLSSLLKSGNATILYEKQNSTKGRPERVYKLFISENKY"
     gene            complement(17678..18892)
                     /locus_tag="L3M60_001375"
     CDS             complement(17678..18892)
                     /locus_tag="L3M60_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBN6226054.1"
                     /translation="MRNCIIAQSGGPTAVINASAIGIYEKNLETKYFDKVYFGINGIE
                     GILNKNIIDSDSLNKDNVLNLKQTPSSGLGSCRYKLADYRINSYDYDRVINILDEYEI
                     DTLFYIGGNDSMDTVMKFSQYCKNTTCKIKFIGIPKTIDNDLLGTDNCPGFGSAAKLI
                     STLIMETYLDFSIYKNNGIFILETMGRDTGWLAASSSLARLNDTPLVDLIYLPEIDFY
                     EDKFIEDVRKIFKEKNKAYIVASEGIRDKYGNFICEQKTSAHDKFGHSQLGGVSNYLK
                     NLIVSSSITTRVKTLELGVIQRCAMHLASGTDLKQAADVGAYGIELSKKEYTGVMAII
                     NRTCNNPYKYEVGHTDISKIANKTKYFPKEWINEECNYITDEALDYLYPLIQGKPDIK
                     YKNGLPYFSIIY"
     gene            19300..20016
                     /locus_tag="L3M60_001376"
     CDS             19300..20016
                     /locus_tag="L3M60_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentose-5-phosphate 3-epimerase"
                     /protein_id="HBN6226055.1"
                     /translation="MKHIKIAAGLAHVNYGHISAVVKEAMDAGVDYVHSDAADMHDLK
                     NMQLMGGHQIIEAIRPVTDKPIECHIYTRDCDRLFIEKIAAAGCNLLIIPAEHFLGAP
                     LAYIINYCREFGMKVGLTVGCYTPLCFVDEAIYDIDRLQIVVHGVDETDGKDNWGWRK
                     SAVDLVKRARKMIDEKNPKCELAIDGGLRADNMEPLIECNPDVVILSSAIFKDKDGIA
                     AGVKNCRKAIDEAATKFGLE"
     gene            20151..20777
                     /locus_tag="L3M60_001377"
     CDS             20151..20777
                     /locus_tag="L3M60_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBN6226056.1"
                     /translation="MKKEMIKDYLLITVSAFLMALAVNFFFAEHTLAPGGITGLSVVL
                     STFLSMPVENISLGISMPLLVMGLLFLGKGFGIKTLYITLMGPFFLKVIPQTHITDNL
                     LVAGVIGGLLVGTAIGIAIIRGCSTGGTDLVAMLINKVIKFLKLPVILFMLDGFIIVA
                     SGIISKNFMISIYSLISLLLIIKTIGFVTTKFGKNTEEINNINSTCTQ"
     gene            complement(20961..22505)
                     /locus_tag="L3M60_001378"
     CDS             complement(20961..22505)
                     /locus_tag="L3M60_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6226057.1"
                     /translation="MNKRLLDIAELLLNSSDYITVDTIAEKLNVSNKTIRNDLVILDE
                     WLLEFNLSLDKKTGSGVIILGNEDIKLKVIRDINDKSNCIYAYSPEDRKRYILSKLFI
                     KNSKFRIRDICNELHVSRATVHKDLVVIQNFLQNFKVTLVRKTNNGVYLEGKEKDIRK
                     AIFELATVNKSYTELKEILFSKSNECKDTPSTKIFKELFNYDFEKLSQITLQTLQVEK
                     SNLSDEYYINFLIHIAICIRRISIKRYINLSEGFFRELLEHEYFEKSKILCSNLGEAF
                     DVEFISEEVCYILLHVEGLLKSIKKTTTLEPLKNEEITEYNLSEAIAVHWGNILNLNL
                     KDDLILIKSLANHLKSVLHRINYGFTITNPILNDIKRTFPYTYKAAKESNTVIKQLMN
                     YEVNDDEIGYLALYLISAIDRSKSPLNTILICHCSDGVSNLLVQKLSFEFNQINIVKS
                     IPLSSISFTNFDDVDLILTTAPVDFEHNAELININALLSKNDISRLSTIIKKLYSEKN
                     KILSYK"
     gene            complement(22531..23172)
                     /locus_tag="L3M60_001379"
     CDS             complement(22531..23172)
                     /locus_tag="L3M60_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBN6226058.1"
                     /translation="MKYLIDSANLDEIQKCLKCGFKGITANPSMYLKENINFYEFIET
                     CTNLNPEILTAEVIGNNLYELINSCDKILNINKDTIIKINFSEIGLELINILNSKGVK
                     TACTLIFNVNQASLAINAGADYLFPFIGRNDENGNDGIKNLTEICDLISINGYNTKVV
                     AASIKNVFHLTQASLCGCDYAAVTYDLFKKASFNQLTVDGANTFERDWSKVNK"
     gene            23443..24594
                     /locus_tag="L3M60_001380"
     CDS             23443..24594
                     /locus_tag="L3M60_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBN6226059.1"
                     /translation="MINFYQPSRINFGQGTISELPRIIGKYGKKCLLVTTPNVEPLDK
                     LYKRVTLDLIEKGIEVVHFDRVEPNPTVELINEGFELAKKESVDVVLAVGGGSSIDTA
                     KVLALTFGLEKIDWDYMFSTYTNPFKIYDKVSEKELPLIAVSTTSGTGSQVTQAAVIS
                     RGSEKNTIFHQNCFSKECIVDPELMLTLPERITASTAFDAFTHAFESYINPNANDFTE
                     IMSEKSMELVINYLPKVMEDKNNIEYRTKLAVADTLGGSSLANAGAAAPHPLSEIIGG
                     VTHIPHGEALAIVFPKFAEKFYKDNVDKFAKVARMLNQELEKVEDNEAAKTLGKEIER
                     FLKNVGLKTKMSEFNVTKEQLEQITSCPVLGFLPFGKKEDLVEILEKSY"
     gene            24607..25419
                     /locus_tag="L3M60_001381"
     CDS             24607..25419
                     /locus_tag="L3M60_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBN6226060.1"
                     /translation="MRYKLICTDMDGTLMGKGFEVSEENIKALKEAMEKGIKVALVTG
                     RPYNAIKYFTSVLGDDIYIISTNGTYFKLLGYEYKKVLSKEALKKIYTIGEKYNLNKH
                     FKGCKIVISNNEIGQEHPYRLINSKNKEEDRIEIIENASCETLLEKADNEILKCILFS
                     ENVESLREAKEEFKKQEDLEVVSSGKINFEVMSKGTSKGIAVKKFCDILGIDSKEVIC
                     IGDNENDISMIEFAGLGIAMGNATDEVKSIADFVTDTNVNDGVAKALRKVLS"
     gene            25445..25936
                     /locus_tag="L3M60_001382"
     CDS             25445..25936
                     /locus_tag="L3M60_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6226061.1"
                     /translation="MFNIFKKKENKVEKIYSPFKGNLVNLEDVPDAVFAQKMMGDGVA
                     IIPSEGKLYSPVDGEIVSVFDTKHAITIKSNDNTNMLIHVGLETMSLEGKPFDVKVKA
                     GDKVKAKDLIMEVDLDFITSNDLNIITPVVIMDDADSVKRTVKDRIEPGNVDINSIIM
                     EVE"
BASE COUNT         9951 a         2887 c         4322 g         8861 t
ORIGIN      
        1 atatatacat aatattatct taaatattaa aagagtatac tgcataaaaa ataataatca
       61 cttatgagta ttccataata actcataaat gattattatt taagaataaa gtacgtaact
      121 gaataagaag attaagaaaa gttttagctt tgttaagaat aatttaagta tataaatgtg
      181 tatggggtgg aattagttga taaaaatagc aatatgtgaa gatgaaaaag aacaacaaga
      241 gttgttgaaa agatatataa atcaaatatt tgatgcattg tctgttaaat gtagattgga
      301 agtatttaat tcaggggaag aattacttga aggatattca aaagatactg atgtactttt
      361 attagatata caaatgggac aaattaatgg aatcgatact gcgagaaaag ttagggtact
      421 ggatgacaaa gtagaaatta tatttattac agcattaata gaatatgcac tagaaggtta
      481 tgaggtaagg gcttatagat acttaataaa accagtaaag tataataatt taaaagataa
      541 tataataaat tgtataaaag aagtggatat aaaaaataaa tatattatgg taaaagcaca
      601 agggagtcaa actaaattaa atgtaaatga aataacatat atagaagtgc aaaaagaaac
      661 tataactata cacactttaa atgaagttta taaaataaat ggcacaatga gtaatattga
      721 aaaggaaata gattgtagta gattttttag atgtcataag agttttttgg ttaatttaga
      781 gcatgtgaaa agtataaaac agtatgttgc tatacttgaa aacagcaagg aagtacctgt
      841 tagtaggtat aggtttaagg agacaaaaga taaatttttt gatttaatag aggagaaatt
      901 atgttagata gtgtgtatag cttagctatt ataaatatgg taatatttat agctttattt
      961 tttatgttga tgaataaaat tggttatact tacagatgca aaactaatat tcaacttata
     1021 ttaattttaa tattatctgc aatcttaatt ataaatggta tgccttttgg gattgtgcca
     1081 ttacatgctt taataagaat aattttaata ttatctttaa tttgtaaaat tacttatcag
     1141 attaacataa aaaaagctac tattaattct gttatatata gcttgttata tattttgata
     1201 gggtgtagta tgtattggat tttacagttc tcatggaagt atgtaggtca ttacatactt
     1261 aaagcaaata ctgtaataaa ttttattatt atttttatag gtgtttattt ttttgatata
     1321 ctacagaaaa tttataataa taagaagtat tatttatata taattttaac aatagcagtt
     1381 aatgtagtta tagttttatt tgtaaatata tcaactgaaa aaatggatga tttatataca
     1441 atagtaacaa agaatcatat tgaatatagt tataatgtat atacaatgta tttttcactt
     1501 tttatgaaaa atacatttcc atatatttta tttatagtaa atgtattatt gatatctata
     1561 tttataaatt ctataaaatc agaaaaagaa aaaattaaag cagaatttgt aagtgaaaag
     1621 ttagatatgc agtataaata ctattttatg gtaaaagaat cacaagaaaa aatgagacaa
     1681 gtataccatg atatgaacaa tcatatgaaa aatataaaat cgctaaaaaa cagtagtgag
     1741 gacgtaaaca aatatattaa tagtatagaa gacgaagtaa aaaatactaa aaatatttat
     1801 aatacaggaa atgtactatt agatattatc ttgtatgaaa aaagtagatt atgcatggag
     1861 aataatatag attttaatgt aggcatagat tttagtaaat gtgaatttat agaaatgatt
     1921 gatgtaagta gtatattttc aaatttaata gataatgcaa tagaagcatg tagtaatatt
     1981 gatgacaata atatagaaaa atatataact ataaaaagta cctttataaa agggtattat
     2041 ataatcagat gtgaaaacag taaaatgaat aaagtaataa ttaaaaataa taagattttt
     2101 acttctaaaa aagataaatt ccttcatggc ataggtattg agagtataaa atcttcaata
     2161 agaaaataca acggagaatt aaaaattaag gatagtgagt ataaatttat agttactgcc
     2221 catataccaa ttgaataaaa attcaataga ttgatgcatt taaaaagctt ataagctagt
     2281 ctttaatatg ctagttgacg gtgaaaaaat accacttgtc ggacttatat tgaataaaaa
     2341 ttttaaaaat gttaccttta caaaaaaggt agcatttttt atacaaggat ttaatagaag
     2401 aaggaggtaa agttttgact acatttaaac tagcagtttc ttatatgaaa agacaaaaag
     2461 gaaaaacagt agcactacta tcgtgtatag ttcttgctgt aatgcttatt ttttctatga
     2521 ttgtgattag ggattctgga tatgattccc aaataaaaga ggcaaaggat ttacatggtg
     2581 attatcatat atggtttgag ggactcgaaa aggataaaac acaagacctc acaaatgaag
     2641 aaggtatatc aaagtcaaac acttcaaaag agctatgtga agttgtagat aaagaaagtg
     2701 gtgttaagct ttatttaaat tcatttgaca agggttttct aagttctttg ggatataaaa
     2761 tagagggaag agaacctata aaagatggag aaatagtaat agaaaaagaa gctacaaggc
     2821 aaatggggat atctaaccca ttaaacaaaa atatagattt gatgctttta aataagtata
     2881 ttgatgacaa tgagattaac catatggata gtgcaaataa aacattcaaa gttgttggtg
     2941 tgatagaaaa attagataaa tattacgata aatctaggta tagtattaca cttcgagctt
     3001 ttgtgtataa agactccaat ttgccaataa aaataaaaga tacgtataat ggaacaattt
     3061 atcttaaatc agagaaaaat atacctcaat ttataactaa gatgacaaaa aagatagata
     3121 ccaatgtctt taatctgcat gaaaatggtg aggttgactt agcaaagaat caaagacaaa
     3181 attcaaaatt cagcaaagaa aatattataa attcagtgct tttagtcagt gtatcaacta
     3241 ttgtaatata caatattttt aatattattt ttcaggatat gactagtcaa ataggtatta
     3301 tgaaatctat tggtatgtca aataagaaag taagaaatat gcttattatt atgagcttta
     3361 tctatataat tttaggaact ctagtaggaa tagtatttgg aatgattttt tcatatgttg
     3421 gtttaagagt agtgtatgga tacagctcta tgctaacaat acaaatatct agtgtaatat
     3481 gttcttttgc agtttcaatc atatcagtat ttatttctag ctttatagta attaaaaaat
     3541 tcaggaaaat gtctataatt gaggctataa ggtcaagtga taagtatgaa aaaaaatcaa
     3601 aaaatgacaa taaaaagata gatggaaaaa gtaaaaattt acttataagt atggcaacta
     3661 gaaatctatg gagaaataaa cctagaacaa tattaacaat attagcaatt acattggttg
     3721 gaataatgtt tatcttaaat ctaggtgcta aaagtttgct aaaaaagaat atggaagagg
     3781 ggataacagg tggttcatgg tcaatgtcat atggaagtgt tgataaaact gttgagggag
     3841 attctagaag ttcagaatct ttattttata aattagacaa taatttaata caaaaagtga
     3901 acggcatgaa aggtgtaagg catgtagaac cacattttta taatcatcgt ggccatattt
     3961 tactttctaa aaataagtta tcaaaagctt ataaagatga attagacaga aaaaattcct
     4021 cttatcaaga ggaacataac aatgaatatc cacttttgat aagagggtat agtgatgata
     4081 tgttaaagca aagacaagaa tttattgaaa aaggggaaaa tatactaagt actacatctg
     4141 gcaagtacaa gaaagtcatc ttagttaaca atacgaattc acaagttaca cattcttttg
     4201 atgcaaagat aattgatgat gtgaaaatag gagatattat agagataaag ctacctgtat
     4261 atagagatgg tattgaaaaa tatgaaaact ttaaggtaga ggttggtgct atcatgaaag
     4321 aattatatgc atcaagtcag gatgggaata cacaagctca aggggctcaa atgatattta
     4381 aagaaaatga ctataaagaa ttaacaggtc aaaaagagta taataagctt tttgtcatag
     4441 ttgaaaaggg acaattatat tctgtagaaa gaagattaga agaaaccact aagaattatg
     4501 gatctactga aatcaatgga aaaggagaag aactaaaact tattggaatg caacaaagtt
     4561 cagaagaaag actttctgta atatatcagt ttttgactat attaatactt gcggtgaata
     4621 ttatttttat tatgagaagt aatattatta caagaagaaa agagctggca accttaaggg
     4681 caattggaat gagtataaaa agtattaaaa agatactaat tattgaaagt caaatgtatg
     4741 gaatggtagc ttctataata ggagctataa ttgcaacagt atatcataat tatggtttgg
     4801 ctaaaacgaa caaagttctt ttggaaggtg gatatactag gataatagaa cccaatattc
     4861 ctttttctca aataattata ctatttacaa tttttattgc tatgggattt atttctatat
     4921 atgtatcaaa agataagata gaaggtacaa caataacaga aggaatttca caaaataatt
     4981 aaggctatgg aatgacaaga taaaaatgta atactattta gtgggattta aataagaaga
     5041 atggagaacc gtattatgaa aaaattagct ataaagaata taaagaaagt atatggaaaa
     5101 ggggataata aagtatatgc attaaatggg atagatttag aaatacaacc taataaattt
     5161 acaactatag ttgggcaaag tggctcaggc aaaagtacat tacttcactg tatggcaggt
     5221 cttgataaac ctacagatgg aaaagtattt atagatgatt tagatttata taccctaaat
     5281 gatgataagt tatcaaagat aaggagagaa gagtttggat ttatatttca aagttacaat
     5341 ctaattccag ttataaatgt ttatgataat atagtacttc ccatttcaat tagtaaaaga
     5401 aaaatagata aagactatat aatagattta atagctaaac taggtataaa atctcaaatt
     5461 gataaatttc ctaatgaatt atctggaggt caacaacaaa gggtagcaat tgctagagca
     5521 cttgcaaata aaccttcaat aatatttgcg gatgagccta caggaaactt agatagtaag
     5581 acaactaatg aagttgtgaa acttctaaaa ctctgtgtat atgagtataa acagacatta
     5641 gttatgataa ctcacaatgc tgaaatagct aataattcag ataatattat aaatattagt
     5701 gatggtaaaa tagtattaat ttagtatata aactttgcaa gataatcgcc tacagagtct
     5761 gtagttatat aattaaaaaa atatttagga aaccgttgac agatttacat agatagtata
     5821 acatatagga tataaaatta aacgaaaaag aattttaatt atggcgacgt agtcataatt
     5881 ttgagtatgt tactgattag caggcagaac tcaagtatat atgtgaaaaa tagagggata
     5941 ctctatacat atatgcttga gttcttttta ttgaagggag gaatatatga aaataattca
     6001 gattttaaat aataatgtgg ccttggtaaa aagaggcaaa aatgaaatta ttgtatttgt
     6061 taaaggaatt ggctttaaaa aaagagtagg acaagtaata aatgaaaatg aaatagagag
     6121 aacatatata ttagattcat acgatatgtt agaacacttt agttatctat tacaaaactc
     6181 agactccaat aatatagttc tcattaatga aataattgaa tatggtgaga aagttttaaa
     6241 tataaagtca agtgattatc taagtttaac cttactagac catttagagt ttttacttaa
     6301 aagatgtgaa aagaatcaat ttataaaaag tcctttggta tggaatgtta aaagatttta
     6361 tcccaaacat tttgaaattg gaatgtatgc actgaaatta attggtgata gcaaggaaat
     6421 taatcttcct gatgatgagg cagtatcaat agcacttcat tttgtaaata tggaggtaaa
     6481 caaagagtct catgatagta cgattgtaga gttaagaact ttagcagata tagtttctat
     6541 aataaaatat cattttaatg tagagataga tgaaacttca acaaattata tgagatttac
     6601 tactcatcta cagtacttta tccaaagaat tatgtcagat gaagcttcaa tagatgaaga
     6661 taattctata gatttatata aacaagtaag tcaactttac ccaaagtcat ttggaagtgt
     6721 tcaaaagata agaaaatata taaaatcaca atttaatgta gatatatcta taagtgaaga
     6781 aacttattta atgttgcata taaatagggt gactcaaaga ctggaggcaa aatcagatga
     6841 aatataaaga tttaaatgaa aatataataa aacttacagg gggaaaagaa aatataaagt
     6901 cggtagcaca ttgtgttact cgtttaagac ttactttaaa tgataggagt atcgctcaaa
     6961 cagaagaaat taaaaaatta aaaggagtaa ttgatgtagt ttctaatgat gttgcttatc
     7021 aaattataat aggtactcat gttgtagatg tatataacga gtttatgtct atgataggtt
     7081 taagttcaag tgaatcaagt gtaaaaggtg aaagaaaaaa gggaattaga ggtatcaaag
     7141 gtatatttaa ttccatcttt gtggtagtct cagaaactat gacttcagta attgaagtac
     7201 tacttgcagc tggtatactt gcaagtatat tagcgctttt aaatatgtct ggtatacttc
     7261 atagtgatag tcctacatat caaatattag atacattaag aagcgctaca tttcacttct
     7321 taccagtatt aattgcaata gcatcagcaa aaagacttaa tgtaaatccc tatatagcag
     7381 tagtgttagc agttacatta ctatcttcat caatagatgg tgtagaagga ttaagtttat
     7441 ttggtataaa tttacaggct acaacctatg caaacagctt tattccaata cttttaggag
     7501 tatggtcaat gggaattata attggaggtt tgaaaaaaat actacctaaa gcactacact
     7561 attttttagt tccagtatta tctttaataa taactttatc agttacatta actatatttg
     7621 gacctattgg tatgtggatt ggtgatgtat taaactatgc atgtatgttt ttaattgata
     7681 cattaggaaa ttggagtgtt gttgcattat atgcagcttt acaaccactc ttaatagtct
     7741 ttggcgcagc aaattttact atgccaatag ctttaaattc tgtgacaaca ttagggtatg
     7801 acccaatttt tatgggagca gcaacaatat ctgatattgc agtttgtggt gcaatgtttg
     7861 gatatttctt aaaaactagg aataaagagc aaaagcaatt atttgggaca gtgagtttta
     7921 gtgcacttat gggagtcaca gaaccttcag tgtttggtgt gtttatgaaa tatagaagac
     7981 catttgtagc agtaatgata ggcggaggct taggtggcct aatagcagga ttagcacaag
     8041 ttaaaacata tggaatggtt tggggattag ctgcattacc aacatatctt gttggtggag
     8101 agatgtctaa ctttacattt atgataatag ctgtattagt atcatttgtt ggagcaacag
     8161 tggcttctta tttactagga ataccttcag atgaaaaaga agaggctaag aatgctaatg
     8221 aaaaagaatt agaattagta gttaacgaag ataattcaag gactattagt gttggaagtg
     8281 ttgcaaaagg tcaggtaatt tcacttgagg aagttaatga taaggcattt tcatctgggg
     8341 cattaggtaa aggggttgga gttataccta gtgaatcaaa atcaactgtt atgtctcctg
     8401 tagatggaac tataactata gtatttcaga ccaaacatgc ctatggtata gtgactgatg
     8461 aaggattaga aatattaatt catattggga tagatactgt aaacttggat ggaaagttct
     8521 ttgaaagtct tgtaacacaa aatcaaaggg ttaaaaaagg agatgcttta gcagtatttg
     8581 actctgataa aattatagag gcaggatttg attcaactat aataactgta gttacaaata
     8641 ctagcgatta tttggatgta atctcatcaa atgatgaatc agaagaacta ttaactgtaa
     8701 tagtataaat tttaggaggg gaaatttatg aatattaaag atacattttt ctggggagga
     8761 agtattgcag cccatcaatg tgaaggttca tgggatagtg ataacaaagg acctgcaatt
     8821 atggattttg taactaaagg ttcttatgaa acaccgagag taattactga taaaatagaa
     8881 gaaaaattgg attatccatc tcataatgga atagattttt ataatagata taaagaagat
     8941 atagctttat ttaaagaaat gggatttagt gcattaagaa tatctataga ttggtcaaga
     9001 atttttccaa atggggatga tgaaaaccct aatgaattag ggattaaata ctatgaatgt
     9061 ctgatagata ccttaataga aaataatata gaacctattg taactctata tcattttgaa
     9121 ttacctatga acttagtaca taaatatggt tcatggaata acagaaaact tatagatttg
     9181 tatttaaagt acagtgagac tgtaataaaa aagtttgatg gcaaggtaag gcactgggtt
     9241 acatttaatg agatgaacca tattgaccct caaacagagc actcggatat ttttacttat
     9301 atgatagcag gtattaagta tagtgaaata gaaaataagg ttcaaacact ggcaaacgta
     9361 gggtataata tgactttggc aggtgtaaag gttgcaaagt tggctagaga aatcaatcca
     9421 aacaataaag ttggatgtgt atttggacta aatccaattt atccatacaa ttgtaatcct
     9481 agtaatgtgt taaatgcatt tttagataat gaccgtgatt attatcaaat tgatgcaatg
     9541 tgcaatggga agtttcctca atataaatta aaagaatatg aaaaaaataa tataaatatt
     9601 gaagtgactg atgaggacaa aaaagcattt gaagaaggta gattagattt tataggtctt
     9661 aattattatt tttcaagtgt atctactcca aaagaaaatt tatcaggtga taaatcttta
     9721 tttggtggta ttcaaaatcc atatttggaa cagagtaagt ggggatgggc aatagaccct
     9781 gtaggtttaa gatttacttt gaactatatt tacagaaagt atcaattacc tatcttaatt
     9841 acagaaaatg gtttaggggc atatgataaa attgaagaag atggaactat aaatgacaat
     9901 tatagaattg agtatttaag taagcatcta tctcaaatga agtctgcaat tgaagaggac
     9961 aatgttgaat gttttggata tttgatgtgg ggaccaattg atttggtcag tgcaactaca
    10021 ggtcagatga gtaaaagata tggatttata tatgttgact tagatgatga aggcaaagga
    10081 agttttaaga gatataagaa aaaatcattt gattggtaca aagaggtaat aaaatttaat
    10141 ggagagaatc taaatttgct agatgtataa aatgaaatat gtgttatatg tattatgatt
    10201 ataccttcga ttattataaa ctacaaataa atttaattta ccatttttcg gtaagtcact
    10261 aaaataataa agttaaacaa gcatttataa gtttagttaa tgtttaaagt aaaagcactc
    10321 ttttaaagta ttatctatat atcagtaatg ttttaaatga gtgcttatgt aataattttg
    10381 ggttaaatat agaataaaaa attaaaagca gtttacttaa aaataaagta attttaattt
    10441 ttatgtattt atactctaag aatatatcct tccttacgag aagcagcctt agggtgtgga
    10501 caatcaggat atccaagagc tatagatgct acacctttta aagtttcagg taagccccat
    10561 ttttttaata attcttttcc ttctattgat tcaaacattt cttttgcacg atgtatccag
    10621 caagaaccta aaccaactgc atatgcagca ttcattaaat tgccagcaac taaactacag
    10681 ttttcaactg gaataagtgc atcttctgga gcaagaataa gtaaaatagt aggtgcacca
    10741 taataaggat gaggtgtttt ttctatatta agtatcctag cattaagtgc gtctaactga
    10801 gaaactgtat cttcgtcttg taccgctatt attattggtt cttgtgagcc tttgctagta
    10861 ggtgcatatg taccagcttc aagaactaaa tcaagctctt ctttacttac ttgatttgat
    10921 ttgaattttc ttatacttct acgatttttt aaaacttcta aagcttcttt tttcatattc
    10981 atacactcct tatgttatag tttaattgta gtcttattag ctacaattaa actataacat
    11041 aaaataattg tagcgtcaat agctacaatt gacaatttat tttataaatt atataatact
    11101 gaaaagggag ggatgccaaa tgcgtatgag cactcagttt acagtatcag ttcaaatgct
    11161 attattaata atggttttag atgatagaaa agtaactagt gaaatgattt ctaaaagtac
    11221 tggaggaaat cctgtaatgg tacgtcaatt gtttggcaaa ctaaaggaag caggaatctt
    11281 aaatgtatct gcaggtagag gagcaacagt tctagcgaag aagccttcag agatttcatt
    11341 atgggatatt tatatggcag ttgaagagta tagttcaagt gaattattta aatttcatcc
    11401 taaaatatca gagggatgta atataggacg tttctttaaa gagatattag gtaatcattt
    11461 agatgaagcg atacatgcaa tggcagataa gatgtctaag gtgtctttag ctcagttgct
    11521 agaggaatgg gaaagtgtaa ataaggagag taagtaataa ttgaacttat ttattgttga
    11581 tattattgaa atattaatat tattaaactt gaaggtcata ttgagagatt atcaatatgg
    11641 ccttatttat acatttagta atggcatcta tataatttta cttataatct atatcaaaca
    11701 agttaatata tataagtatt ttatctatga cttttattct tttaataata tatatataaa
    11761 tttctagcaa gaaatagttt acatttcact actgtggtgt gtatactact atgaatattt
    11821 aaacttttcc tatagaactt taggcatatt aatatattaa tgattaatat gtttgatttt
    11881 taaataatac taaatctaat aaagaattat gttagatgga aaaataaaaa aactataaat
    11941 tatataatag attagtggtt atagagtaaa aataagatgg cagtaaactt taaggtatat
    12001 taataagaag ccacatcttt aattttagca taagattatg aaatcataaa tttacaaatt
    12061 attgtcagaa tacattgaca aatttatggt ttgaaattat aatctatgtt aagaattgtt
    12121 tgacaatgta aaactagtga ttttagaatt tagaagaatt attttttatg tctaatttag
    12181 gaataattta agactattat aggtttataa tttatcatta atttaattag ataaataggt
    12241 tttgtctcta ggaataagat ttctaaaaac actagaaaga gtataattta taaagggggg
    12301 agtagaagag aaaaaagtat atctacagaa agacttaaaa caagacatat tttaataatg
    12361 ttaatgaatc tgtttttaag atttgttaag gattttaaga ataatttaag actcttataa
    12421 aatgggattc ttatagttag aatttagata agtataagtc ttctaagaag tatataaaaa
    12481 ttaaaaatag ttatagcgta attctaaatt tgtaaaaaga tgtataagat taaaaagaaa
    12541 ttaagtatat gaataactat ggttaagatt taagaaatta accgtggtta tatttattca
    12601 gaaattttaa gaataattta agacgcttaa aaatcatcag aaaatatcaa acttaccata
    12661 tgtagcccca aatagctata gagtgtacat tttatgaata ttctttcatt tataaaaaat
    12721 tattttattt aggaggaaac aacaatggaa aatttttgga atccgcttac ttgctttact
    12781 gtatgtgtaa tgatttatgc ttttagtgag tttttatcta agaaaacaaa aggtgcaatt
    12841 tcaacattgc tatttgtatg tattatattt ttaataggat tttggacaaa tattttaccc
    12901 aaagacatta caggagcatc agggatatta gctgcaatga gcaactttgg tatagcttta
    12961 ttaataacta acttaggaac acttataaat cttgaagact tatgtcatga atggaaaacg
    13021 gttgtaattg cattaatagg tattgtagga ataggggttg gctgtttaac agtaggtagt
    13081 atgttatttg gtagagagta tgctttaata gcagctcctc caatagcagg ttctactgtt
    13141 gctggtatta tcgtaacaac tgttgcagaa gctgcaaata gacctgaact tgctgcattt
    13201 gtaattctag tattatcatt tcaaaagttc tttggcattc cgataactac atattgtata
    13261 aataaagaat taaaatttaa aagagataat ggggattttt tgaaagatga gtcaaaaagt
    13321 aattttaaac ttccaaacat gagaattttt aaagaagatt taggtaaaaa atcatctact
    13381 ttagtgtatc ttgcaaagct ttcatttgtt gctatgattg gtaattttgt aggattggct
    13441 actttaatac caggatttac acctgcaaac tattatttaa atcccaacat agcatgtttg
    13501 ttagtggggt tgatttttac acgaataggt tttttagaaa aaggaatttt agataaatct
    13561 caatctaatg ggattataat gtttggttgt atgttaatgt tacctggagg acttgctaag
    13621 gtaactccaa cagcattgtt tgggatgatt gttccaacta taggaatact tgttgtaggc
    13681 tccgtattta taatattatt tacttcaatt gtaggcaaac tattgggata ttctccaaga
    13741 atgtcagctg caattggtgt aacttgtatg cttgcttatc cagcaacaca gataatttct
    13801 aatgaaggta ttgatgcaat ggaaggaact gaagaagaaa aacaaagagc aacagattat
    13861 ttgttaccaa agatgattat aggaggattt gttactgtaa ccattgcatc agtagcattt
    13921 gctagtataa taggtccaat tatatttggt tgatagtaaa tagaattata ggaggaatag
    13981 gaaatgatag gaagtatatc tataaatgag atagataaaa aaagattcaa acttgatgat
    14041 ttgagtagga aaatatggga aaatccagaa aaagcattta aagaatttaa agcatgtgaa
    14101 aatacagcaa attttttaag aagtgaagga tttgatgtag aaataggtgt aggaggttta
    14161 gccacggcta ttaaagctag ttttggttca ggcaaaccag ttataggttt tatgggagaa
    14221 tttgatgcgt tacctggttt aaatcaaaaa gtttcaacaa agcaagaagc atttgaattg
    14281 ggtgcttatg gtcatggatg tggacataat ttattatgta cagcacatgt aggtgcagta
    14341 gtagggatta aaagagaaat gatagaaaat aacttaggtg ggactatagt attttatgca
    14401 tgtccaggag aagaacaatt aacaggtaaa ggatttatgg ctagaggtgg agcttttgaa
    14461 ggtttagact tagctataaa ttttcatcca aataaaatta gtgaagctac aattggtact
    14521 tcaacagctg ttaattctgt aaaattccat tttaagggaa aaactgcaca tgcaggctca
    14581 gaccctcaaa atggaagaag tgcactagat gctgtggaac ttactaatgt aggagctaac
    14641 tatttgagag aacatgttac ttctgatgta agaatccatt atacaattac agatggagga
    14701 gtagcaccaa acatagttcc tgataaagca tcagtatggt attacataag ggctttaagt
    14761 agagaagctg tagagagtac atatgaaaga cttgtaaagg tagcaaaagg agctacaatg
    14821 atgactgaaa ctgaagttga agtagagttt ttaggaggat gttataatac tttaaataat
    14881 catactttag caaatcttgt atcagaatgt atgaatgaga ttaaacaaga gccttggact
    14941 caagaggaat tggattttgc agctgaatta gatagacaaa gcccacaaca atataaggat
    15001 atgataagta aatataattt gcaagaagga actcatctat attatggtgg aggaaatgtt
    15061 acaaacttta atagttatgg ctctactgat attggagatg taatgcatat agttccaaca
    15121 gcatactttt ttacagggtg tacgaatcta ggtgcgccag gtcatagctg gcagtttaca
    15181 tcttgtgcag gaagttctat aggagaaaaa ggtatgatat atgcagcaaa aattatggct
    15241 atgtttggag ctaaaatatt aaacaatcca gagatagcta aaaaggcaaa agaagaattt
    15301 gataaatcta tgaatggtaa aacatataaa tgtcctattc cagatgatat tccaacgcct
    15361 taaaaagata tgaagtttaa tatataaaat tataattttt atgtcaacat catttcatac
    15421 taaaagaaag tgagatttta ctaagagttt aggagtattt ttatggattg gaatgggaag
    15481 tattagtcag ggaaataatt ttggtatgca taatcctaag gcaattttta atgaataggg
    15541 attagtttac ggttcagctt tatttgctaa ttatgcagta aaatggttag aaaataattt
    15601 tagtagagaa taacataaaa tattattaaa tatcataaaa ggtatatctt aaaaacaaat
    15661 gtcaagatat accttttaga gaattatata taattttact taatatcaga tgataaaata
    15721 tttttatgta ttttggattt taatatttat tttctgatat aaataatttg tatacccttt
    15781 caggtctacc ttttgttgag ttttgttttt catataatat agtagcgttt ccagacttta
    15841 agagactact tagaaatcta ttagcagttc tcactgttac atttaaacat gaagacaatt
    15901 ctttaggagt aacttcatct ctttgtagaa tttcagtaac agatataatc ttttgaatag
    15961 taagagtaga caatttagaa cttttagcaa ttgtatttat atgtggagtt actttatttg
    16021 aaacagtaag taacttatca cataataaag gtcctattgt ctcataattt tcgttaacta
    16081 agaaactgta gtttaaaggg tttattttag attccttatt agcacttatg gcactagaca
    16141 tagcttgttt tatatcatta cctattccat atccaataca taccttaata tttatactat
    16201 tttcaatgga ttcttttata taacacgtag taaaattttt agttaatgat ttaatcgttt
    16261 cacatttagt gaaaatttta aaagaattac tatttttttc aattataaaa tcggttaact
    16321 tatctttttt tatactattt aatattttat aaacttggtc taaagtagaa tcatcataat
    16381 ttgtatcttt aactgttata tatattacac ttggttgatt tcccttcatt ctatgtaaat
    16441 gaatgtcttt tattaagtta ttaaaagtat tttttaataa atctatatca ggatatatga
    16501 aatgaaagtt tactcctgca tcttgtaaca taggaacaat actactaaaa cgagtagtag
    16561 aaaaatcaat tttcccttca ttccatagat taatatgttt ttttgcaatc tttttttcga
    16621 gttttgctat atcagataaa ctcattttag atgcattttc atcaatacta tccattaagt
    16681 ctccaaatgt accatttgat aaataatatg atatatcttt tggattatct gcccagtcag
    16741 ttaagtcaaa gtatgctcta gaaaaatcta atgtattata tttaaagata agctttagaa
    16801 acatatcata gtaactttgc aaatcagtac caaaatcaac tagaggtttt ttaatagttc
    16861 cagccttttt tgtaatagct tttttgggga taggtccact aactgcaaat ccatcaaaac
    16921 tatcttctat ttctaaatac aaatctccaa catgagaaaa atctctatag atatatattt
    16981 caatttctgc attaatattt aattttttta tagaattatc tacaaactct tttaaaaatt
    17041 cggttgttat gactgctatt ttcatcatag attacttcct tttcaattat tataattatt
    17101 ttatattaaa tattacatta atctattgcg ctagttaaaa tcatatttca attaaaataa
    17161 ataactcttt ataaaaaaca attatttaga tagaaaaata tttacgaact ttaatagtct
    17221 ttgttaactt tgattttatt taagttatta tagactcgct aattttataa ttgtagattt
    17281 agctacttta tgttctttta ttgtttatgt tgttgattta gaagttattt taagtactta
    17341 ttttaattga agttgtttgt tttttaaaat cctttgtgta atctttattt ttctagccat
    17401 aataaaaata tttgtatttg tatcactata tataataata tcatgacaaa atatttattg
    17461 tctaactaat tataatctat tcaaaatgaa aatgggaatg agatattatt acaacaagtc
    17521 gtattattat taaaattgat taaataatta aatgattaaa taattaaatg attaaatgat
    17581 taagtgactg ttatataaca atttgtaatt ttatttaaca gccacttagg tatttaagag
    17641 caatggaatt gagtgtatat caaattataa taatttccta gtaaataatt gaaaaataag
    17701 gtaatccatt tttatatttg atatctggct taccttgaat aagtggatat agataatcca
    17761 gtgcttcgtc agttatataa ttacattctt catttatcca ctctttaggg aaatactttg
    17821 tcttgttagc tattttagaa atatcagtat gaccaacttc atacttataa ggattattgc
    17881 atgttctatt aatgatagcc ataactcctg tatattcttt tttagatagc tctatcccat
    17941 atgcaccaac gtcagctgct tgttttaaat cagtacctga agctaaatgc atagcacatc
    18001 tttgtattac accaagttct aaagttttta ctcttgttgt aattgatgat gatactataa
    18061 gattctttag atagttgctc actccaccta attgagaatg accaaattta tcgtgagcag
    18121 aagttttttg ttcgcatata aagttgccgt atttatctct tataccttct gatgcaacta
    18181 tataagcctt atttttttct ttaaatattt ttctaacatc ttctataaac ttatcttcat
    18241 agaaatcaat ttctggaagg tatattaaat caacaagtgg agtatcatta agtctagcta
    18301 gtgaagaaga agctgctaac catccagtat ctctacccat agtttctaat ataaatatcc
    18361 cattattttt atatatagaa aaatctaagt acgtttccat tattagtgta gaaataagct
    18421 ttgcagcaga accaaatcca ggacaattgt cagttcccaa taaatcatta tctatggttt
    18481 taggaatccc tataaatttt attttacaag tagtgttttt gcagtattga gaaaatttca
    18541 ttactgtatc catagaatca tttccgccta tgtaaaataa tgtatctatt tcatattcat
    18601 ctagtatatt tataactcta tcataatcat aagaatttat cctataatca gctaatttgt
    18661 atctacaaga accaagacca gatgaaggtg tttgctttaa atttaataca ttatctttgt
    18721 ttaaagagtc tgaatctatt atatttttat ttaatatacc ttcaatccca ttgataccaa
    18781 aatacacttt atcaaagtat ttagtttcta aatttttttc ataaatacca attgcagaag
    18841 cattgataac agctgtagga cctccagatt gggcaataat acagtttctc attttacatc
    18901 tccctttcat taatattgtt atgaatgata ctttttattg agcaaattta tatttatagt
    18961 ctgattatat attaagtgaa ctagctatat caattgagtt ggtatagata ataaatacca
    19021 taagaatgtg gcaaaaaact aactttttat atacaattaa tgtctataat ttaccacaat
    19081 ataaaggtac ttttaaggag tagtcaaagt gttgtgaaaa gaaaaatatc agtgtaatat
    19141 taatttacaa agcaaaataa gttcacagtt cacaaaagca gaataagttc ataaaagaaa
    19201 tacatacagc aataaaagta atactaaatt aaaaaaatta aaaaatatca aaaactaata
    19261 tttaatatta attaaagtaa aatatggagg gacacaaata tgaaacacat aaaaatagca
    19321 gcaggattgg cacatgtaaa ttatggtcat atatcagccg tagttaagga agcaatggat
    19381 gcaggggtag actatgtaca ttcagatgca gcagatatgc atgacttaaa aaatatgcaa
    19441 cttatgggtg gtcatcaaat aatagaagca ataagaccag tgacagataa accaatagaa
    19501 tgtcatatat acacaagaga ttgtgataga ttatttatag aaaaaatagc agcagcagga
    19561 tgtaacttgt tgataatacc agctgaacat ttcttagggg ctcctcttgc atacataata
    19621 aattactgta gagaatttgg tatgaaagta ggattgacag ttggatgtta tacaccacta
    19681 tgttttgttg atgaagctat atatgatata gacagattgc aaatagttgt tcatggtgtt
    19741 gatgaaactg atggaaaaga taactgggga tggagaaaga gtgctgttga cttagtaaaa
    19801 agagctagaa aaatgataga cgaaaagaat cctaagtgtg aattagcaat agatggtgga
    19861 ctaagagcag acaatatgga gccattgata gaatgtaatc cagacgttgt aatattatct
    19921 tcagcaatat tcaaagataa agatggtata gcagcaggtg ttaagaattg tagaaaagct
    19981 atagatgaag cagctacaaa gtttggttta gaataagata ataaactatg ataaaagaga
    20041 tgctttctta atgttttaag gaagcattaa ttttatgaaa aactacagga agttagacta
    20101 atttaattga ttttttatac tgaaagaata taatcattgg aggttattaa atgaaaaaag
    20161 aaatgataaa ggattattta ctgataacag tatcagcatt tttaatggca ttagcagtta
    20221 attttttctt tgcagaacat acattagccc caggagggat aacaggatta tcagtggtac
    20281 ttagtacatt tttatcaatg cctgttgaga atatatcatt aggaatatct atgccacttt
    20341 tagtaatggg attattattt ttagggaaag gttttggaat aaaaacatta tatataactc
    20401 ttatgggtcc attcttctta aaagtaatac cacaaactca cataactgac aacctattag
    20461 tagcaggagt gataggtggg ttattggtag gaactgctat aggtatagca ataataagag
    20521 gttgtagtac tggtggaact gatttagttg caatgcttat taataaagta attaagtttc
    20581 tgaagttgcc agttatatta tttatgctag atggatttat aatagtagcg tctggaataa
    20641 taagtaagaa ttttatgata tctatatatt cattgatatc tttattactt ataataaaaa
    20701 caataggatt tgtaactaca aagtttggta agaatactga agaaattaat aatataaata
    20761 gtacttgcac tcaataaaaa taataaaact tactttaaat ataaaagttt aaataagaag
    20821 tttaaatgta aaaatggagg tgtctcaaaa taaatttttt agttcacgag gcactttttt
    20881 tctatgaaat caaatatatt tttatcatgt agacaatgaa aactaggctt atctttatgt
    20941 tgagacaagc ctagttttgt ttatttatag gataatattt tatttttttc agaatacaat
    21001 ttttttataa ttgtactaag tctggaaata tcattcttgc ttaaaagagc atttatattt
    21061 atcagttcag cattatgttc aaagtccaca ggagcagtgg ttagtattaa gtccacatca
    21121 tcaaagttcg taaatgaaat agaagataga ggtatagatt taactatatt aatctgatta
    21181 aattcaaaac ttagtttttg gactaataag ttagatacac catcactaca atgacatatt
    21241 aaaatagtat ttagaggaga tttacttcta tctattgctg atatcaaata taaagcaaga
    21301 taccctattt cgtcatcatt aacttcataa ttcatcaact gtttaataac agtattagat
    21361 tctttagcag ccttatatgt ataagggaat gtcctcttta tatcatttaa tatgggattt
    21421 gtaatagtaa atccatagtt tattctgtgt agaactgatt taaggtgatt agcaagagac
    21481 tttattaata ttaaatcatc ttttaagttt aaatttagta tatttcccca atgaacagct
    21541 attgcttcag atagattata ctctgtaatt tcttcatttt taagaggttc aagagtagta
    21601 gttttcttta ttgatttgag caatccttct acatgaagaa ggatataaca gacctcttca
    21661 ctaataaatt ctacatcaaa agcttcacct aggttagaac ataaaatttt tgatttttca
    21721 aagtattcat gttccaataa ttctctaaag aaaccttcag ataagttaat atatctttta
    21781 attgaaatcc tcctaataca tatagctata tgtattaaga aatttatata atactcatca
    21841 ctaaggttac ttttttctac ttgcaaagtt tgtagagtta tttgagataa cttctcaaaa
    21901 tcatagttaa ataactcctt aaatattttt gttgatggag tatctttaca ttcattagat
    21961 tttgagaata atatttcttt aagctcagta taacttttat taacagtagc aagctcaaaa
    22021 attgcttttc gaatatcttt ctcttttccc tctaaatata ctccattatt ggtttttcta
    22081 actaatgtta ctttaaagtt ttgtaaaaaa ttttgaataa ctaccaagtc tttatgcaca
    22141 gtagctctgc taacatgtag ttcattgcaa atatcacgaa tcctaaactt agaattttta
    22201 ataaataatt tacttagaat ataccttttt ctatcttcag gagaatatgc atatatacaa
    22261 tttgatttat cattgatatc tctaataact ttaagtttta tatcttcatt acctagaata
    22321 ataactcctg aaccagtttt tttatccagg gataaattaa actctaaaag ccattcatct
    22381 aaaataacaa ggtcattacg aatagtttta ttactaacat ttaatttttc ggcaatagta
    22441 tctacagtaa tatagtctga gctgttgagt aataattcag caatatctaa taatcttttg
    22501 ttcaatgcaa accctcctaa atcttagtta ctatttatta accttggacc aatctctttc
    22561 aaaagtattg gcaccatcta ctgtaagctg gttaaaagaa gcttttttaa ataaatcata
    22621 tgttacagct gcataatcac atccacaaag agatgcttga gttaaatgga atacattttt
    22681 tatagaagct gcaacaactt tagtattata tccatttatt gatataaggt cacatatttc
    22741 tgtaaggttt tttataccat cattcccatt ttcatcattt ctacctataa aagggaatag
    22801 gtaatcagca cctgcattta tagctaatga tgcttgattt acattgaaaa tcaatgtaca
    22861 tgcagttttt acacctttac tatttaatat gtttataagt tctaagccaa tttcagagaa
    22921 atttattttt attatagtgt ctttatttat atttaatatc ttatcacatg aattaattaa
    22981 ttcgtataaa ttatttccga ttacttcagc agttaatatt tctggattta gattagtaca
    23041 tgtctcaata aactcataga agtttatatt ttctttaaga tacatagatg gattagctgt
    23101 tattccctta aatccacact ttaaacactt ttgaatttca tcaagattag cactgtctat
    23161 caaatatttc ataatttaat tctcctttaa gcttatttgt cataaaaaag tgtatatact
    23221 ctatttttat tattaagtca attatacaat aagattgatt ataaaattat aacatgggta
    23281 cttaataaaa tttacataat gatatggtaa taataaaaca ttgattgtct aataattgcc
    23341 ataagcttta gtaaataaga gttaacagtg aaggtattgc cttaaaaatc ctatgatatg
    23401 tataatttga atatgaaggc aataatttaa gggaggaaaa atatgattaa cttttatcaa
    23461 ccatcaagaa taaattttgg acaaggaact ataagtgagt tacctagaat aataggaaaa
    23521 tatggaaaga aatgtttgtt agttactact ccaaatgtag agccattaga caagctatat
    23581 aaaagagtta cattggattt aatagaaaaa ggtatagaag ttgtacactt tgatagagta
    23641 gagccaaacc caactgtaga attaataaat gaagggtttg aattagctaa aaaagaatct
    23701 gtagatgttg ttttagctgt tggtggaggt tcaagtatag atactgcaaa agtattagca
    23761 ttgacttttg gtttagaaaa aatagattgg gattatatgt ttagtacata tacaaatcca
    23821 tttaaaatat acgataaggt aagtgaaaaa gaattaccat taatagctgt atcaactact
    23881 tctggaacag gaagccaagt tacacaagca gcagtaatat cgagaggaag cgagaaaaat
    23941 acaatatttc atcaaaattg tttctcaaaa gaatgtatag ttgacccaga gcttatgcta
    24001 actcttccag agagaattac tgcatcaaca gcatttgatg cctttactca tgcgtttgaa
    24061 agttatataa atccaaatgc taatgacttt acagagataa tgtctgaaaa gtctatggaa
    24121 ttagtcatta actatttacc taaagtaatg gaagataaaa ataatataga atacagaaca
    24181 aagttagcag tagcagatac attaggcgga agttcactgg caaatgctgg agcagcagca
    24241 ccgcatccac taagtgaaat aataggagga gttacacata ttcctcatgg cgaggcttta
    24301 gctatagtgt ttccaaaatt tgcagaaaag ttctataaag ataatgtaga taaatttgct
    24361 aaagtagcga gaatgttaaa ccaagaattg gaaaaagtgg aagataatga agcagctaaa
    24421 actttaggaa aagaaataga acgtttttta aagaatgtgg gacttaagac aaaaatgtct
    24481 gagtttaatg tcacaaaaga acaattagaa caaataacga gttgtccagt tttaggattt
    24541 ttaccttttg gaaagaagga agaccttgtt gaaatattag aaaaatctta ttaggatgtg
    24601 atacaaatgc gatataaatt aatttgtacc gatatggatg gtactttgat gggtaaagga
    24661 tttgaagtat cagaagaaaa tattaaagca ttaaaggaag ctatggaaaa gggcattaag
    24721 gtagccttag tcacgggaag accttataat gctataaaat attttacaag tgttttagga
    24781 gatgatatat atattatatc aactaatggg acatatttta aattactggg atacgagtat
    24841 aagaaggttt tgagtaaaga agctctaaag aaaatatata caataggtga aaaatataat
    24901 ttaaataaac attttaaagg ttgtaaaata gttatatcca acaatgaaat aggtcaagaa
    24961 catccatata gattaataaa tagtaaaaat aaagaagaag acagaattga aataatagaa
    25021 aatgcaagtt gtgaaacttt attagaaaaa gctgataatg agattttaaa atgtatactt
    25081 ttctcagaaa acgtagagtc tttaagagaa gcaaaagaag aatttaaaaa acaagaagat
    25141 ttagaagtag taagttctgg aaagataaat tttgaagtta tgagtaaagg tacttcaaaa
    25201 ggtattgcag ttaaaaaatt ttgtgatata ttaggtatag atagcaaaga agttatctgt
    25261 ataggtgata atgaaaatga tatatccatg attgaatttg caggtcttgg tattgctatg
    25321 gggaatgcta cagatgaagt aaaatctata gcagactttg taactgatac taatgtaaat
    25381 gatggtgtag caaaagcttt aagaaaagta ttatcataaa attttataat gtggagagtg
    25441 tgttatgttt aatatattta agaaaaaaga aaataaggtt gagaaaatat acagcccttt
    25501 taagggaaat ttagttaact tagaagatgt tccagatgca gtttttgcac aaaagatgat
    25561 gggagatgga gtagcaataa taccaagtga aggtaagtta tattctcctg ttgatggtga
    25621 aatagtcagt gtgtttgata caaagcatgc aataacgatt aagagtaatg ataacacaaa
    25681 tatgttgata catgtaggtt tagagacaat gtcactagaa ggaaagccgt ttgatgttaa
    25741 ggtaaaggca ggagataagg taaaagctaa agatttaata atggaagtag atttagattt
    25801 tataacgtca aatgatttaa atataataac tcctgtagtt ataatggatg atgctgattc
    25861 tgttaagaga acagtaaaag ataggataga acctggaaat gtagatatta atagcataat
    25921 aatggaagtt gaatagatat aaaaagggag ttgtctcaaa atttatttta ctaatcaaat
    25981 tttaagttca acttcctttt tggatgtgtt tttttgatat t
//
LOCUS       DAFNNB010000037        25720 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0037, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000037 DAFNNB010000000
VERSION     DAFNNB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25720
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0037"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1340
                     /locus_tag="L3M60_001383"
     CDS             <1..1340
                     /locus_tag="L3M60_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6226062.1"
                     /translation="VKNIMFTIEENMDIRLTDISFKQLMLFLLINIIRIRLGNSLKVS
                     NTKKELKLKMLILDIEDELKSNLSIVLNYEEKLFIESLILGMNKQINSSNIKKSLYLN
                     EELANYTRKIINLVSEESGINFNNDILLYEQLICHLNVTMHQMKSNVYLENPLLDNIK
                     TKFCFLFTVISSSIEIELKGKQITEDEIGYLTLHFQTSLERKYSKRESNKKASIVCPF
                     SFGVSMLLKVKIEKRFDNIKIIETLREEDLKRDNFNKTIDFIIAFQEYENIQKPIFIT
                     TPLFTDEDENKLKEFTNKIKEKDTSYKLMNRLMMSDYLIRELEHDDIYEAITYLTNLL
                     IEKHYAEKEYLQSIITREKSYPTNVGKGILFPHGDMKYIKQSVLCFARLKTPIKIRNG
                     KDIKIILLLAYKKDDDRKVFRELFKEISNLTEDENMLDILSKCDIEKVKDILI"
     gene            1481..1789
                     /locus_tag="L3M60_001384"
     CDS             1481..1789
                     /locus_tag="L3M60_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBN6226063.1"
                     /translation="MEEIVLKIIIHAGNAKSMLYEALDYAKENDFKKADELIENANEE
                     ILKAHKVQTELIQKEAGGDKSDISILLIHSQDHLMTCMSERNLIKEMIMLRKEIQKLK
                     "
     gene            1814..2140
                     /locus_tag="L3M60_001385"
     CDS             1814..2140
                     /locus_tag="L3M60_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6226064.1"
                     /translation="MKRKVYLFCSFGMSTSLLAEKMQKVADEHNLPIEVEAFPIAEID
                     KIVEEKNPDCILLGPQVKYMLKELKPKFEAQGKLIDIINEVDYGTMNGEKVLKLAIKL
                     IKSKQA"
     gene            2180..3514
                     /gene="celB"
                     /locus_tag="L3M60_001386"
     CDS             2180..3514
                     /gene="celB"
                     /locus_tag="L3M60_001386"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HBN6226065.1"
                     /translation="MNRLERILMPLAEKIGKNKLLIAIRDGFLISSPLLIIGSLFLLV
                     SNFPIKGWNEFVGQIFGPDWAVKLQQPTVATFEVMTLLAVLGIGYSYAKQIDVDPIAS
                     AAVAIVAFFIITPFVIPYTPEGTESVYMVTGIPLGWMGSKGMFVGMITAITSVKLFAA
                     VVKKGWTLKMPDGVPPTVSKSFAALIPSAIVMVVFFLVKIAFEATPYGSVHEFIFKFL
                     QIPLLKLGNTLSATAIAYVFLHLFWFFGINGSSVVGAVYNPILKILSAENLAAFQAGT
                     KLPNIITAQFQDMFATFGGAGSTLSLIIAMLLICKSKRIKSIGKLSILPGIFGINEPL
                     IFGLPIMLNPILLIPFALVPTVNIIIAYFCMSTGLVPLTNGVQLPWTTPIIFSGFLTT
                     GWQGAVLQLVLIILGIFMYIPFIKMLDKQYLKDEKSNVEGEDDDISFDDLVL"
     gene            3525..4043
                     /locus_tag="L3M60_001387"
     CDS             3525..4043
                     /locus_tag="L3M60_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBN6226066.1"
                     /translation="MKKIVMILDQIQAGAGGKEKSNIPPAGKSSPLGPGVMMEPFLNE
                     SKVIATLFCGDEFFINNEEEVTSKMIAMVKKLNPDVVICGPSFNYENFSKMSAILSKN
                     INDKADIPAFAAMSEENIDVINEYKNDICIVRTPKKGGIGLNDSLNNICKLAKAMANK
                     EDITLMKEEFCY"
     gene            4139..5098
                     /locus_tag="L3M60_001388"
     CDS             4139..5098
                     /locus_tag="L3M60_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HBN6226067.1"
                     /translation="MWGMIATWRMALEGITEASEHLEKGMEAGDAVELAVRRVEDYPF
                     YKSVGYGGLPNENCEVELDAAFMDGNTLDIGAVAGIRDYSNPVSIARKLSYEKVNNFL
                     VGIGAEEYAHKNGFERKNMLTDRAKLHYKKRKKETLDKGLSPYAGHDTVGMISLDKTG
                     KMCAATSTSGLFMKKRGRIGDSALSGSGFYVDSSIGGATATGLGEDLMKGCISYEIVR
                     LMKEGKHPQEACDIAVNELDKQLIKRRGKAGDLSLVAMNNKGEWGVATNIADFTFSVV
                     TKEESPTVYISNRVDGKTIHKVISNEWMEAYEARIKAPIEVSE"
     gene            5147..6199
                     /locus_tag="L3M60_001389"
     CDS             5147..6199
                     /locus_tag="L3M60_001389"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBN6226068.1"
                     /translation="MNIISNKIDELKEDLLSDIIDIVKIPSVKGESENGFPFGEKVGE
                     ALNKALEISEKLGFKIRNLNNYIGYAEHGDSEDYVCVIGHVDVVHEGDGWKYQPYKGE
                     EINGRIYGRGVLDNKGPIISALYGLYAIKELNLKLDKRVRIIFGTNEESGFEDIPYYL
                     EKEKAPIMGFTPDCKYPVVYGEKGMAKIRIKSKINYEEDVLLGFIENVSENVLVTYKE
                     LNIENSDTILDIKVKYDFSYKLKDVLDEIKFSFPNSIDIEVISNFNPVYFDKESNLVK
                     KLQLAYERVTSLDGTPVTTNGGTYAKVMPNIVPFGPSFPGQKGIAHNPDEYMDIEDII
                     LNAKIFANAIYELAKE"
     gene            6202..6876
                     /locus_tag="L3M60_001390"
     CDS             6202..6876
                     /locus_tag="L3M60_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBN6226069.1"
                     /translation="MLEIIGMSVEDAKIIEDCGADRIELVSALTEGGLTPSFGLIERV
                     VNSVKIPVNVMIRPHAKSFVYSKEDINIMQKDVSVVKEIGANGVVFGVLDKNNNIDEK
                     NLNVLLKCCDNLDVTFHRAIDESNTIDSVKTLKDYDKIINILTSGGKGSIVDNIQIIK
                     NMMLSSNHIKILLGGGLNFNNIEKIKELTKASNFHFGTAIRMNNSPFEDIDRQKLRQL
                     VNIISR"
     gene            7429..7980
                     /gene="raiA"
                     /locus_tag="L3M60_001391"
     CDS             7429..7980
                     /gene="raiA"
                     /locus_tag="L3M60_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBN6226070.1"
                     /translation="MNIIISGKQIELTDAIKNKIESKLSKLDNYIHPDTDVKVTVSAR
                     KARQKIEVTIATVNGPIIRAEDMQENLYTAIDVVYDKLSKQLKKYKKRLQDKHKDNKS
                     IRFQDAEINLADEQEDYGDENQELVIQRRKKFSVKPMSEEEAVLQMELIGHDFYMFKN
                     IDSDEIAVVYKRNNGGYGIIEHE"
     gene            8112..10787
                     /gene="secA"
                     /locus_tag="L3M60_001392"
     CDS             8112..10787
                     /gene="secA"
                     /locus_tag="L3M60_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBN6226071.1"
                     /translation="MSFMDNLFNMADKKELKKFNKTVDIIDSLEPKFESMADSELKNM
                     TNIFKERLANGESIDDILPEAFAVVREVSKRVLGLRHYRVQMIGGIVLHQGRIAEMKT
                     GEGKTLVATAPVYLNALTGKGVHVVTVNDYLAKRDRDQMAKIYEFLGMSVGVIIHGQN
                     PKVRKEQYDCDITYGTNNEYGFDYLKDNMVIHKEQRVQRGLNYAIVDEVDSILIDEAR
                     TPLIISGPGDKSTHLYSDANTFVLTLKPDDYELEEKDKAVSLTASGIQKAEVYFNVDN
                     ITDISHTELYHHINQALRAHVIMKKDVDYVAKDGEIVIVDEFTGRLMFGRRYSEGLHQ
                     AIEAKEGLKIQRESKTLATVTFQNYFRMYKKLSGMTGTAKTEEEEFKAIYKMDVFQVP
                     TNKLMIREDLPDCVYKSEIGKFNAVAQEIIERHKVNQPILVGTVSIEKSELLSQILKK
                     KGIKHEVLNAKHHDKEAEIIAQAGRLGAVTIATNMAGRGTDIVLGGNPDFLTKREMRR
                     NGFKEEIVNRVDTPIEGIPVKGNEILFEAREEYEKLFEKFKQQTQEEQKQVVEAGGLA
                     IIGTERHESRRIDNQLRGRAGRQGDPGSSRFYIGLDDDLMRLFGSDRISGIVDKIGLE
                     EDMPIEHRILSKSIEGAQKKVEGKNFGIRKHVLQYDDVMNKQREIIYAERKRVLEGED
                     LQEQIQSMTHSIIEEAVTLYTQDKGFDEEGFKEHMYNLFLPKGSIEIPEIEKLNPVEI
                     TEKVYEIAMKIYTSKEEQVGYERMREVERVILLQAVDNHWIDHIDAMDQLRQGIGLRA
                     VGQQDPVIAYKMEGFDMFDEMNKHIKEDTVRYLFNITIETPVERKAVVDVENLSSPSD
                     GTLPISKTVKKDEKVGRNDLCPCGSGKKYKNCCGR"
     gene            10802..11909
                     /gene="prfB"
                     /locus_tag="L3M60_001393"
     CDS             join(10802..10876,10878..11909)
                     /gene="prfB"
                     /locus_tag="L3M60_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153309863.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBN6226072.1"
                     /translation="MLKLQEYRHSMEQMASNLEELRVSLDIASLMKNIQINEEKMNKQ
                     DFWNDNEVAQRVLQENKSLKETLEEYESLKSLLEDIEVLIEIGLEEEDDSVERDIEKS
                     IESMEEKLSEMKIKTLLNGEYDKNNAILSINAGTGGLDAQDWAQMLLRMYIRWSESKG
                     YKVKLLDIISDPEAGIKTATILVEGTNAYGYLKSEKGVHRLVRISPFDPSGKRHTSFA
                     SIDVTPELDENIEVEINPSDLKIDTYRASGAGGQHVNTTDSAVRITHIPTGVVVQCQN
                     ERSQHLNKDRAMRLLMAKLIELKELEQKEKIEDIQGKYSQITWGSQIRSYVFQPYKLV
                     KDHRTNAEFGNVDSVMNGNIDLFINEYLKMNKIV"
     gene            12054..14195
                     /locus_tag="L3M60_001394"
     CDS             12054..14195
                     /locus_tag="L3M60_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBN6226073.1"
                     /translation="MDINQILKKEFNLRDEQINNTLKLIDEGNTIPFIARYRKEMTGE
                     MSDVTLREFYEKLMYLRNLQSRKDDVVRLIDEQGKLTDEITQNIEKAKTLQEVEDIYA
                     PYKQKKRTRATIAKEKGLENLALSILENNLDNIEIEAENYLDEEKEVLSIEDALKGAR
                     DIIAELVSDDAKIRKYIRELALREGMIVSKSATDEKSVYDMYYDYSEAVKSMAPHRVL
                     AINRGEKENFLKVKLEINNDKVLNYIINEYVNDKNFKNKEEIVNSIEDSYKRLIFPSI
                     EREIRNHLTEIAQERAISVFGKNVKSLLLQPPVKDKVVMGFDPAFRTGCKIAVVDKNG
                     KLLDYTTVYPTDPQNDVEGAKKVLKGLIEKYDIDIISIGNGTASRESETFVSEMIKEI
                     DREVQYVIVSEAGASVYSASELANEEHPDINVSIRGAISIARRLQDPLAELVKIDPKS
                     IGVGQYQHDLNKKRLEEVLDGVVEDSVNSVGVDLNTASYSLLEHIAGISKAIAKNIIA
                     YREENGDFTSRAQLKKVKRLGPQAFTQCAGFMRILEGKNPLDNTGVHPESYDICKKMI
                     EIIGYSLDDVKNKNIDEIDEKIKEIGLKELSEKLEVGQVTLKDIIAEIKKPGRDPREE
                     GIKPILRTDVLKIEDIQEGMILKGTIRNVVDFGAFVDIGIKNDGLVHKSEMSNSFVKD
                     PMSIVTVGDIVDVKVIGIDLNKKRVALSMKK"
     gene            14343..15509
                     /locus_tag="L3M60_001395"
     CDS             14343..15509
                     /locus_tag="L3M60_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226074.1"
                     /translation="MIFIKNGKINTITNGIIHGDILIEEGKIIEIGEDLIAPLDVEVI
                     DASNKLVFPGFIDAHTHLGLWEDGIGFEGADGNEETDPITPQLNPVDGINPMDRTFKE
                     AFEGGITSVCTTPGSANVMGGQCIAIKTYGKRIDKMVIKNPVASKIAFGENPKSCYGQ
                     DDKSPQTRMAIAALLRENLKKAEEYLEDIDMYESHDDEDCEKPEYDIKMESLIPVLRR
                     EIPFKAHAHRADDMFTAIRIAKEFNLKLTLDHCTEGHLIADELVEEGFPVIVGPSLSE
                     RSKFELRNLTFNTAGILSNAGLDVCIMTDHPVIPVQYLPICAGIAVKHGMKEEKAIES
                     ITINPAKTLGIEDRVGSIEVGKDADLVIWDNSPLEIQSNVIYTIINGKVVYEKK"
     regulatory      15569..15686
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            15810..16415
                     /locus_tag="L3M60_001396"
     CDS             15810..16415
                     /locus_tag="L3M60_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBN6226075.1"
                     /translation="MQNTAKRGNMISTRTLVVMSILSAISYLLMFIQAPIPGIFPDFL
                     KIDLSDIPAIFGGMSLGPLVGFGIVVVKNVLQAITATTTGGIGEFANILIGGSYVMIL
                     CYSYKRSGDLKGVLIGFVLGTISMTIIGSLVNYFVMLPLYGQLMGLDAIIGLGSAINP
                     QVHDLFTFVIWMIAPFNVLKAVIISLVTLPLYKKMGNIIKK"
     gene            16745..17785
                     /locus_tag="L3M60_001397"
     CDS             16745..17785
                     /locus_tag="L3M60_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBN6226076.1"
                     /translation="MNLSKKIKKLFLDDKGYYFNSNWKDIHIGIYDTKLGDSLKYITN
                     EYIENLERVKYDYRELKNIENQTDGMCILAIRQNYKALEYVKSQTEELCLEAIKYNYK
                     ALEYVKEQTEYICLEAIKKDCNALKYVRNKTEGLIIKVIRHSTNIDVVSILKALETQT
                     RRICLEAIKKDGRCLAYVREQSEELCIEAIKQNYKALKYVENQTEKMCIEAVKQNGMA
                     LQYVNKQTDKICIEAVKQDGRSLQFVDNKTEEICINAIRYLNKKYNIKDVLCYVDNYT
                     EDMCIEIVRQNGKMLMYIKNQTEKICIEAVKENYKSLKYVKEQSERICKEALKQNYKA
                     KEYVKIAIDDCI"
     gene            17924..18376
                     /gene="tsaE"
                     /locus_tag="L3M60_001398"
     CDS             17924..18376
                     /gene="tsaE"
                     /locus_tag="L3M60_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBN6226077.1"
                     /translation="MAKIYLENENKTREIGYKLGKLLKEGSVICLVGDLGAGKTTMTQ
                     SLADSLGIKDYITSPTFTIINEYEGKIPLYHFDVYRIGSSDEMYDIGYDEYVNSNGIC
                     IIEWANLIEDILPKEYLNIELRYKDEGREMILTPKGEFYKEIVEELIK"
     gene            18376..19092
                     /gene="tsaB"
                     /locus_tag="L3M60_001399"
     CDS             18376..19092
                     /gene="tsaB"
                     /locus_tag="L3M60_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBN6226078.1"
                     /translation="MKILGMDTSSMAASVAVVEDDNLICEFTANNKKTHSQKLMPMIE
                     NMLSMSDLSIKDMDLLAVCIGPGSFTGLRIGMATVKAMAHVNNIPIIAVNSLESLANN
                     MNFCDRKICCILDAQKNQVYSAKYKFENGEMIELDGVDVIEFDALVEEIVSTNEEFIM
                     VGEAVYKYKDKLENIENIKIPSPANNVSKAGSLCSLALNKYNKNIDVHTCYTINPMYI
                     RKSQAEVQYDEKMKRLNNGR"
     gene            19082..19558
                     /gene="rimI"
                     /locus_tag="L3M60_001400"
     CDS             19082..19558
                     /gene="rimI"
                     /locus_tag="L3M60_001400"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBN6226079.1"
                     /translation="MEDNINLIVNDIKIEEMTIEDIDEVFEVEKNCFEDYWSKESFRK
                     ELSNEVARYIVAKLDGKIVGYVGIWLILDEGHINNVAVHSDYRGKKIGDKLIKGIVDL
                     CKDNNIASMTLEVRASNKIAQNLYRKYGFKMGGIRKEYYSDNKEDAIIMWNQLKEV"
     gene            19564..20580
                     /gene="tsaD"
                     /locus_tag="L3M60_001401"
     CDS             19564..20580
                     /gene="tsaD"
                     /locus_tag="L3M60_001401"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBN6226080.1"
                     /translation="MSDIITLAIESSCDETAASVLKNGREVLSNIISTQIETHKKFGG
                     VVPEVASRKHVENIDIVVQEALDKANMDFNDIDHIAVTYGPGLVGALLVGLSYAKALA
                     YTLNIPLVGVNHIEGHLSANYIEHKDLKPPFITLIVSGGHTHLVEVKDYGKYEILGKT
                     RDDASGEAFDKISRAMNLGYPGGPIIDNLAQKGNKHAIEFPRAYLEEDSYDFSFSGLK
                     SSVLNYLNGKRMKNEEIVVEDVAASFQEAVVEVLSTKALKAVKDKGYNIITLSGGVAS
                     NSGLRAKITELAKDSGITVKYPPLILCTDNAAMIGCAGYYNFINGKTHDMSLNAVPNL
                     KINQ"
     gene            20877..23585
                     /gene="hpdB"
                     /locus_tag="L3M60_001402"
     CDS             20877..23585
                     /gene="hpdB"
                     /locus_tag="L3M60_001402"
                     /EC_number="4.1.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase large
                     subunit"
                     /protein_id="HBN6226081.1"
                     /translation="MSQSKEDKIRSILEAKNIKSNFQNKENLNEFNEKKASKRAEDLL
                     DVYYNTLSTADMEFPYWYNREYRKSDGDIPVVRRAKALKAAFSHMTPNIIPGEKIVMQ
                     KTHHYRGSFPMPWVSESFFVAQGEQMREEAKKLASNTADELTKFGSGGGNVTESFGNV
                     VSIAGKFGMRKEEVPVLVKMAKEWVGKSVEDLGFHYEKMMPDYDLKENLMSTLICMFD
                     SGYTLPQGREVINYFYPLNYGLDGIIEMAKECKKSVAGNASGDGLIGMDRLYFYEAVI
                     QVIEGLQTWILNYAKHAKYLESIETDLEAKKEYSDLVEILEHIAHKQPRTFREALQLT
                     YTIHIASVNEDAISGMSIGRFGQILYPWYEQDIEKGLITKEEVIELLELYRIKITCID
                     CFASAGVNGGVLSGNTFNTLSIGGLREDGSTGANELEELLLEASMRCRTPQPSLTMLY
                     DEKLPEDFLMKAAECTKLGSGYPAWVNNSNGTTFMMKQFADEGMTVEEARAFALGGCL
                     ETSPGCWKQLTLNGKTYSIAGGAGQSAGSGVHFIANPKILELVLMNGKDYRMNIQVFE
                     PHNKPLDTYEEVIEVFKDYYKQAINVLERANNIELDIWRKFDTSIINSLLKPDCLDKG
                     QHIGNMGYRYNATLNVETCGTVTMVNSFAALKKLVYDDKAFTIEEMKDAILNNFGFKD
                     ALEVGNYSMADQVKVDKTGKYDAIYKACLDAPKYGNNDLYADNILKNYEVWLSKVCEE
                     AQSLYAKKMYPCQISVSTHGPQGAATLATPDGRLSGTTYSDGSVSAYAGTDKNGVYAL
                     FESATIWDQAVVQNSQMNLKLHPTTIKGQQGTKKLLDLTRSYLRKGGFHIQYNVVDSE
                     TLKDAQKNPDNYRQLMVRVAGFTQYWCELGKPIQDEVIARTEYEGV"
     gene            23589..23846
                     /gene="hpdC"
                     /locus_tag="L3M60_001403"
     CDS             23589..23846
                     /gene="hpdC"
                     /locus_tag="L3M60_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase small
                     subunit"
                     /protein_id="HBN6226082.1"
                     /translation="MRKHSDCMNFCAVDATKGICRLSKQMINLDDEACPEIKVMPKCK
                     NCKNFVEANDEGIGKCVGLEKEDWVYSTLNAITCEGHVFNE"
     gene            23839..24789
                     /gene="hpdA"
                     /locus_tag="L3M60_001404"
     CDS             23839..24789
                     /gene="hpdA"
                     /locus_tag="L3M60_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate decarboxylase activase"
                     /protein_id="HBN6226083.1"
                     /translation="MSSQKKLEGMIFDVQSFSVHDGPGCRTTVFLNGCPLSCKWCANP
                     ESWTVRPHMMFSELSCQYENGCTVCHGKCKNGALSFNLDNKPVIDWNICKDCESFECV
                     NSCYYNAFKLCAKAYTVDELVQVIKRDSNNWRSNGGVTFSGGEPLLQHEFLHEVLLKC
                     HEVNIHTAIETSACVSSEVFNKIFEDIDFAFIDIKHMDREKHKEQTGVYNDLILKNIS
                     NLANSDWNGRLVLRVPVISGFNDSVKNISDIISFMHKNNLVEINLLPFHRLGESKWVQ
                     LGKEYEYSDKGDVDEGHLEELQDIFLDNGIACYVGHETAF"
     gene            complement(24897..25133)
                     /locus_tag="L3M60_001405"
     CDS             complement(24897..25133)
                     /locus_tag="L3M60_001405"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226084.1"
                     /translation="MTSVYKKTNDNVYLYNLEKTLDEIFQLFDLSLRYSFKTFSNLET
                     MENTSFLEGTSGIILLLISISSKDSTYKKLLFLS"
     gene            complement(25153..>25720)
                     /locus_tag="L3M60_001406"
     CDS             complement(25153..>25720)
                     /locus_tag="L3M60_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6226085.1"
                     /translation="KNLYPVKNIKAGSLLIIYFMISVIVPTSFLLILQGTQPSYSGII
                     KFIFTPITSLTTIYIFFSEEYAWRGFLQNIFFDRFGKKLGVIILGMCWSLWHLPLIFT
                     LYTPETPILGLILRSIHIVGIAIFLGYLYMKTKNVWFCAIIHILNNSAFLITNVEESI
                     ITYSDILIVSMVSLIFYVPFLFTKEYKG"
BASE COUNT        10468 a         2698 c         4859 g         7695 t
ORIGIN      
        1 ttgtaaaaaa cataatgttt actatagaag agaatatgga tataagactt acagatatat
       61 cattcaagca attgatgcta tttttgctta taaatataat acgaataagg ctaggaaatt
      121 ctttaaaagt atcaaatact aagaaagagc ttaagctaaa aatgcttata ctagatatag
      181 aagatgaact aaaatctaat ttatctatag tattaaatta tgaagaaaag ctgtttatag
      241 aatctttaat attgggtatg aataaacaaa taaatagcag taatattaag aaatctttat
      301 atctgaatga agaattagca aattatacaa gaaaaattat aaatttagta tcagaagaat
      361 caggaataaa tttcaataat gatatattac tttatgaaca attaatatgt catctgaatg
      421 ttacaatgca tcaaatgaaa agcaatgttt acttagaaaa tccactttta gataacataa
      481 aaactaaatt ttgtttttta tttacagtaa tatcaagttc aatagaaatt gaattaaaag
      541 gaaaacaaat aacggaagat gaaatcggtt atcttacact tcactttcaa acctctctag
      601 aaagaaaata cagtaaaagg gaatcaaata aaaaagcaag tattgtatgt ccatttagtt
      661 ttggagtaag tatgctatta aaagtaaaga tagaaaagag atttgacaac ataaaaatta
      721 ttgaaacatt aagagaggaa gatttaaaaa gagataactt caataagaca atagatttta
      781 taattgcatt tcaagagtat gagaatattc aaaaaccaat ttttataaca acaccattat
      841 ttactgatga ggatgagaat aaacttaaag agttcacaaa caaaataaaa gaaaaagata
      901 cttcatataa gcttatgaac agattgatga tgtctgatta tttaataaga gagttagaac
      961 atgatgatat ttatgaagct ataacatatt taactaattt attgattgaa aaacattatg
     1021 cagaaaaaga gtatttacaa tctattataa caagagaaaa gtcataccca accaatgttg
     1081 ggaaaggaat attattccct catggagata tgaaatatat aaaacaatct gttttatgtt
     1141 ttgcaaggtt aaaaacacca ataaaaatta gaaatggaaa agatataaag attatacttt
     1201 tactagccta taaaaaagat gatgatagaa aagtatttag agaattattt aaagaaatat
     1261 ctaatttaac tgaggatgaa aatatgctag atattctaag taaatgtgac attgaaaaag
     1321 taaaagatat tcttatataa aattcataga gacctatgga taagcacaaa attttccatc
     1381 tgatggaaaa agttgtgcat gttttttgtt ttttataaga tattatatgt ttataaggta
     1441 taaataatct aaaaacagag aagcaattta ggaggaaaaa atggaagaaa tagtcttaaa
     1501 gattataatt cacgctggaa atgcaaaatc tatgctttat gaagcgcttg attatgcgaa
     1561 ggaaaacgat tttaaaaaag cggatgaatt aattgaaaat gcaaatgaag aaatcttaaa
     1621 agcacataaa gtacaaactg aacttataca aaaggaagct gggggagata aatcagatat
     1681 atctatttta ttaattcact ctcaagacca tttaatgact tgtatgagtg agagaaatct
     1741 tataaaagaa atgattatgt taagaaaaga aattcaaaaa ttaaaatgaa aaatttaagg
     1801 gggatttaaa agaatgaaaa gaaaagtata tttattttgt agctttggta tgtcaacaag
     1861 tttacttgca gagaaaatgc agaaggtggc agatgaacac aatttaccaa tagaagtaga
     1921 ggcatttcca atagcagaaa ttgataaaat agttgaagaa aaaaatccag attgtatatt
     1981 attaggacca caagttaaat acatgctaaa ggaattaaaa ccaaagtttg aggctcaagg
     2041 taaacttata gatataataa atgaagttga ttatggaact atgaatgggg aaaaagtact
     2101 aaagcttgct ataaagttaa taaaatctaa gcaagcatag gattataaat aaaaaacaat
     2161 gagagatgag ggtggataga tgaatagatt ggaaagaata cttatgccat tggctgaaaa
     2221 aataggtaaa aataagttat taattgcaat tagagatggt tttttaatat catcaccatt
     2281 attaataata ggttcattat ttttattagt atcaaacttc ccaataaaag gatggaatga
     2341 atttgtaggt caaatttttg gtccagattg ggctgtaaag ttacaacaac caactgtagc
     2401 aacatttgaa gtaatgactc tacttgcagt tttaggaatt ggatattctt atgcaaagca
     2461 aatagatgta gacccaatag catcagcagc agttgcaata gttgcctttt ttataataac
     2521 accatttgta ataccatata caccagaggg aacagaatca gtatacatgg tgacaggtat
     2581 accactaggt tggatgggtt ctaagggaat gtttgtaggt atgataacag caataacctc
     2641 tgtaaaatta tttgctgcag ttgtaaaaaa aggatggaca ttgaaaatgc cagatggagt
     2701 ccctccaact gtatcaaaat catttgcagc attaatacca agtgcaatag ttatggtagt
     2761 tttcttttta gttaagatag cttttgaagc tacaccatat ggaagtgtac atgaatttat
     2821 atttaagttt ttacaaatac cattattaaa attaggaaat actttaagtg ctacagcaat
     2881 tgcttatgta ttcttacatc tattctggtt ctttggaata aatggaagta gtgttgttgg
     2941 agctgtgtat aatccaatat taaaaattct atcagctgaa aacttagctg cttttcaagc
     3001 tggtacaaaa ctaccaaaca taataactgc tcaatttcaa gatatgtttg caacatttgg
     3061 tggagcaggt agtacattat cattaattat agccatgtta ttaatatgta aaagtaaacg
     3121 tataaaatca ataggaaaat tatcaatttt accaggtata tttggtataa atgaaccatt
     3181 aatatttggt ctaccaataa tgcttaatcc aatactattg ataccatttg cactagttcc
     3241 aacagtaaat ataataatag cttatttctg tatgtcaaca ggattagtgc cacttacaaa
     3301 tggagttcag ctaccatgga caactccaat tatattctct ggatttctaa caacgggatg
     3361 gcaaggggca gttttacaat tagtacttat tatactagga atatttatgt atataccatt
     3421 cataaaaatg ctagataaac aatatttgaa agacgaaaaa tcaaatgttg aaggggaaga
     3481 tgatgatata tcttttgatg atttagtatt ataggaggaa gattatgaag aaaatagtaa
     3541 tgatattgga tcaaatacaa gctggagcgg gaggaaaaga gaaaagtaat atacctcctg
     3601 ctggaaaatc aagcccatta ggaccaggtg taatgatgga accattttta aatgaatcta
     3661 aagtaatagc tacattattt tgcggggatg aattttttat aaataatgaa gaagaagtga
     3721 ctagtaagat gatagctatg gtaaaaaaat taaaccctga tgtagtaatc tgtgggccaa
     3781 gctttaatta tgaaaacttt tctaagatgt cagcaatact gtctaaaaat ataaacgata
     3841 aggcagatat accagctttt gcagctatgt cagaggaaaa tatagatgtg ataaatgaat
     3901 ataaaaatga catatgtata gttagaactc caaaaaaagg tggaattggt cttaatgatt
     3961 cgttaaataa catatgtaaa ttagctaaag caatggcaaa taaagaagat ataactttga
     4021 tgaaagaaga attttgttat taatatgagt attaattatt agcaagtttg aatattacac
     4081 tatttataat aagcatatat tattaaaatc tatatagttt aaggagggga attttagcat
     4141 gtggggaatg attgcgacat ggagaatggc cctagaagga ataactgagg catcagaaca
     4201 tttagaaaaa ggcatggaag caggtgatgc tgtagagctt gctgtaagaa gagttgaaga
     4261 ctatccattc tataaatcag ttggatatgg tggacttcca aatgaaaatt gtgaggtaga
     4321 acttgatgct gcatttatgg atggtaacac attagatatt ggagcagttg ctggtataag
     4381 agattattct aatcctgtca gtatagctag gaagttaagt tatgagaagg taaataattt
     4441 tttagttgga ataggtgctg aagagtacgc tcataaaaat ggatttgaaa gaaaaaatat
     4501 gttgactgat agagcaaagt tacattataa gaaaagaaaa aaagagactt tagataaggg
     4561 attaagtcca tatgctggtc atgatactgt tggtatgata tctttagata aaactggaaa
     4621 aatgtgtgca gctacatcaa caagtggatt gtttatgaag aaaagaggta gaataggaga
     4681 ttctgcactg tctggttcgg gattctatgt tgatagtagt ataggaggag ctacagcgac
     4741 tggtttggga gaagatttaa tgaagggatg tatatcttat gagatagtta gacttatgaa
     4801 agaaggaaaa catccacaag aagcatgtga tattgcagta aacgaacttg ataaacagct
     4861 tattaaaaga agaggaaaag ctggtgattt gtcattagtt gcaatgaata ataaaggtga
     4921 atggggagta gctacaaata tagctgattt tactttttca gtagtaacta aagaagaatc
     4981 ccctactgta tatatttcaa atagagtaga tggaaagact atacataagg ttatatcaaa
     5041 tgagtggatg gaagcatatg aggctagaat aaaagcacca atagaagtaa gtgagtaaag
     5101 tagtagttac ataattaaaa tagagtttta taataaggag atttttatga atatcataag
     5161 taataaaata gatgaattaa aagaagattt attaagtgat attatagata ttgtaaaaat
     5221 acctagtgtc aaaggggagt cagaaaatgg cttccctttt ggtgaaaaag taggggaggc
     5281 acttaataaa gcattagaaa tatcagaaaa attgggattt aagattagaa atttgaataa
     5341 ttatattggt tatgcagagc atggagatag cgaggattat gtttgtgtaa ttggacatgt
     5401 agatgttgta catgaaggag acggttggaa atatcagcct tataagggag aagaaatcaa
     5461 tggaagaatt tatggaagag gagtactaga taataaaggt cctataatat ctgctttata
     5521 tggattatat gctattaaag aattaaattt aaaattagat aaaagagtta gaataatatt
     5581 tggtacaaat gaagaaagtg gatttgaaga tataccatat tatttagaaa aagaaaaagc
     5641 acctatcatg gggtttacac cagattgtaa gtatcctgtt gtatatggtg aaaaaggcat
     5701 ggcaaagatt agaataaaaa gtaaaataaa ttatgaagaa gatgtattat taggttttat
     5761 agaaaatgtg tctgaaaatg tgcttgttac atataaagag ctaaatatag aaaatagtga
     5821 tactatattg gatataaagg taaaatacga ttttagttat aaattaaaag atgtattaga
     5881 tgaaataaaa tttagttttc caaatagtat cgatatagag gtaatttcta attttaatcc
     5941 tgtatatttt gataaagaga gcaatcttgt taaaaagctt cagctggctt atgaaagggt
     6001 tacatcacta gatggaactc cagtaacaac taatggaggt acatatgcaa aagtaatgcc
     6061 aaatatagtt ccgtttggcc cttcattccc aggacaaaaa ggaatagctc ataatcctga
     6121 tgaatatatg gatatagaag atataatatt aaatgcaaag atatttgcaa atgcaatata
     6181 tgaattagca aaggagtagt tatgttagag ataataggaa tgtcagtaga agacgctaaa
     6241 attatagaag attgtggtgc agatagaata gagttagtta gtgctttaac tgaaggtgga
     6301 ttgacaccaa gttttggtct tatagaaagg gttgttaaca gtgtaaagat acctgtgaac
     6361 gtaatgataa ggcctcatgc gaaaagtttt gtatacagta aagaagatat aaatataatg
     6421 caaaaagatg taagtgttgt taaagagatt ggtgctaatg gagttgtatt tggtgtattg
     6481 gataaaaaca acaatataga tgagaaaaat ctaaatgttt tattgaaatg ttgtgataat
     6541 cttgatgtta catttcatag agctatagat gaatcaaata caatagatag tgttaaaact
     6601 ttgaaggatt atgataaaat tataaatata ttaacatctg ggggaaaagg aagtatagtt
     6661 gataacatac aaataataaa gaatatgatg cttagttcta atcacataaa aattctttta
     6721 ggtggaggac ttaattttaa taatatagaa aagataaagg aattaactaa ggcaagtaat
     6781 tttcattttg gtacagctat tagaatgaat aacagtcctt ttgaagatat agatagacaa
     6841 aagcttagac agcttgtaaa tataatttca agatagtata taaatataaa atacttagtt
     6901 taaataaaga gagtaagtta attataattt gggtatatga atgttaatag gtattacata
     6961 tgaaattatt gttgagtatg ctttacaact tttaagtagt tttaaatata aataatgaaa
     7021 gcaaagatat agtaacgagg ttttagaggg tttatattta ttaaaaaatt ataaaggtat
     7081 actggcatat aacacatata atttcctaaa attaccatct tatgacataa attcacatat
     7141 taataaacaa ttaatggaga tttttgtgga tagaaatagg agcaaaaata gctttaaata
     7201 aacaaaaaca ggagttatta acagttttat ccacattatc cacaggtgtt tgtaataata
     7261 ttaatccaca taagtataaa tttaactaaa aatgataaat aattctatca attgaataga
     7321 aaaaatcata aaagtaaata ataaaaagag gattatgaga gttcgcgtag aatatattat
     7381 taaagtataa attttcttat actggcataa ttgaagggag atgtacttat gaacataatt
     7441 atatctggaa aacaaatcga attaactgat gcaataaaaa ataaaatcga aagtaaacta
     7501 agtaagttag ataattacat tcatccagac actgatgtaa aagttacagt aagtgcgaga
     7561 aaagcaagac aaaaaataga agtaacaata gctactgtaa atggacctat aattagagca
     7621 gaagatatgc aggaaaattt atacacagct atagatgttg tatatgataa attaagtaaa
     7681 caattgaaaa aatataaaaa gagactacaa gataagcata aagacaacaa gagtataaga
     7741 ttccaagacg cagagataaa tctagctgat gaacaagagg attatgggga tgaaaatcaa
     7801 gaattagtaa ttcaaagacg taagaaattt agtgttaagc caatgagtga agaagaagct
     7861 gtattacaga tggaactaat aggacatgat ttttatatgt tcaagaacat agattcagat
     7921 gaaatagcag tagtttataa gcgtaataat ggtggatatg gtataataga acatgaataa
     7981 agcaatttag atttaatcat attagctatc gataaataaa ttatcgatag ctttttaaat
     8041 taatattaaa agtatagttt ttatggtaaa atgaataata gtacaataaa gaacgaggag
     8101 agatgtaaaa tatgagcttt atggataatt tattcaacat ggcagataaa aaagaattaa
     8161 aaaagttcaa taagactgtt gatataatag attcattaga acctaaattt gaatctatgg
     8221 cagactcgga acttaaaaat atgacaaata tatttaaaga aagattagca aatggtgaaa
     8281 gtatagatga tatacttcca gaagcttttg cagtagttag agaagtttct aagagagtgt
     8341 taggacttag acactacaga gttcaaatga ttggaggtat agtactacat caaggaagaa
     8401 tagcagaaat gaaaacaggt gaaggtaaaa ctttggttgc tactgctcct gtttatttga
     8461 atgcgcttac tggaaaaggt gtacatgttg taacagtaaa tgattacctt gcaaaacgtg
     8521 atagagacca aatggcaaaa atctatgagt ttttaggaat gagtgtaggc gttattatac
     8581 atggtcaaaa cccaaaagtc agaaaagaac aatatgattg tgatataact tatggtacaa
     8641 ataatgagta tggatttgac tatttaaagg acaatatggt aatacacaaa gagcaaagag
     8701 ttcaaagagg attaaattat gctatagtgg atgaggttga ctctatacta atagatgagg
     8761 ctagaacacc acttatcata tctggacctg gagacaaatc aactcactta tattcggatg
     8821 caaacacatt tgttttaaca ttaaaaccag atgattatga attagaagaa aaagataagg
     8881 ctgtatctct aacagcatcg ggtatacaaa aagcagaagt atattttaat gttgataata
     8941 taacagatat atctcataca gaattatacc atcatataaa tcaagcttta agagcacatg
     9001 taataatgaa gaaagatgtt gattatgttg ctaaagatgg agaaatagta atagtagatg
     9061 agttcacagg tagacttatg tttggtagaa gatattcaga aggtttacat caagcaatag
     9121 aagctaaaga aggtcttaaa atacaaagag agtctaaaac actagctact gtaacattcc
     9181 aaaattactt tagaatgtac aaaaaacttt ctggtatgac tggtacagca aaaacagaag
     9241 aagaagagtt taaagcaatc tacaaaatgg atgttttcca agtacctact aataagctta
     9301 tgataaggga agacttgcca gattgtgtat acaaaagtga aataggtaaa ttcaatgctg
     9361 tagcccaaga aataattgaa aggcataagg tcaatcaacc aatacttgta ggtacagtat
     9421 caatagaaaa atcagaatta ttgtcacaaa tattaaagaa aaagggcata aaacatgaag
     9481 tattaaatgc taagcatcat gataaagagg cagaaataat agcacaggct ggtagacttg
     9541 gagctgttac aatagctact aatatggctg gtcgtggtac agatatagtt ctaggtggaa
     9601 atccagactt tttaactaag agagaaatga gaagaaatgg atttaaagaa gaaatagtaa
     9661 atagagtaga tactccaata gaaggtatac ctgtaaaagg taatgaaatt ttatttgaag
     9721 ctagagaaga gtatgaaaaa ctatttgaaa agtttaagca acaaacgcaa gaagaacaaa
     9781 aacaagttgt agaagcaggt ggacttgcta taattggtac agaaagacac gaatctcgaa
     9841 gaatagataa ccagttgaga ggtcgtgctg gacgtcaagg tgacccaggt agttcaagat
     9901 tctatatagg tcttgatgat gaccttatga gattatttgg tagtgataga atatctggta
     9961 ttgtagataa gataggtctt gaagaagaca tgcctataga acatagaata ttatctaagt
    10021 ctattgaagg agctcaaaag aaagtcgaag gtaaaaactt tggaataaga aagcatgtac
    10081 ttcaatatga tgatgttatg aataaacaaa gagaaataat atacgcagag agaaaacgtg
    10141 tattagaagg cgaagattta caagaacaaa tacaatcaat gactcattct ataatagaag
    10201 aagcagttac tctttataca caagataaag gatttgatga agaaggattc aaagaacata
    10261 tgtataatct gttcttgcca aaaggtagca tagaaatacc agaaatagaa aaacttaatc
    10321 cagttgaaat aacagaaaaa gtttatgaaa tagctatgaa gatatatact tcaaaagaag
    10381 aacaagttgg ctatgagaga atgagagaag ttgagagagt aatcttactt caagctgttg
    10441 ataaccattg gatagaccat atagatgcta tggaccaatt acgccaaggt atcggtcttc
    10501 gtgctgtagg tcagcaagac ccagttattg cttataaaat ggaaggtttt gatatgtttg
    10561 atgaaatgaa taaacatatc aaagaagata ctgtaagata tttatttaat ataacaatag
    10621 aaactccagt ggaaagaaaa gcagttgtag atgttgaaaa tctatcttct ccatcagatg
    10681 gaacacttcc aataagtaaa actgttaaaa aagatgaaaa agttggaaga aatgacttat
    10741 gtccttgtgg aagtggtaaa aaatataaaa actgttgtgg aagataatct tggaggatta
    10801 aatgttaaag ttacaagaat atagacatag tatggagcag atggcttcaa atttagaaga
    10861 attgagggtt tctctttgac atagcttcat tgatgaaaaa tatacaaatt aatgaagaaa
    10921 agatgaataa acaagacttt tggaatgata atgaagtcgc tcaaagagta cttcaagaaa
    10981 ataagtcctt aaaagagaca ttagaagaat atgaaagctt aaaaagctta ttagaagata
    11041 tagaagtatt aattgaaata ggtctagaag aagaggatga ttctgtagaa agagatatag
    11101 aaaagtccat agaatcaatg gaagaaaaat tatccgaaat gaaaataaag actcttctaa
    11161 atggagagta tgataaaaat aatgctatat tatctataaa tgcgggtact ggtggattag
    11221 atgctcaaga ttgggctcaa atgcttttga gaatgtatat aagatggtca gaaagtaagg
    11281 gttataaagt taagctctta gatataatat cagaccctga agcagggata aagacagcta
    11341 caattcttgt agaagggaca aatgcttatg ggtatttaaa gagtgaaaaa ggagttcata
    11401 ggttagtaag aatatctcca tttgacccat caggaaaaag acatacttca tttgcatcta
    11461 tagatgtaac accagagtta gatgaaaata tagaagtaga gattaatcca tcagatttaa
    11521 agatagacac gtatagagct tctggagctg gaggacaaca tgtaaatacg actgattcag
    11581 cagttagaat aacacatatt cctacaggtg tagtagttca gtgccaaaat gaaaggtctc
    11641 agcatctaaa taaggataga gctatgagac ttttaatggc aaagttgata gaattaaaag
    11701 agttagaaca aaaggaaaaa atagaagata tacaaggcaa atactcacaa ataacttggg
    11761 gtagtcaaat taggtcatat gtttttcaac catataaatt ggttaaagac catagaacaa
    11821 atgctgagtt tggaaatgta gatagtgtaa tgaatgggaa tatagacttg tttattaatg
    11881 aatacttaaa aatgaataaa atagtttaat aaatatgcat tgtaattaag atatatgtat
    11941 aagaaggtta gtgcttaata tgcacttaca gcacttaggc tgaggacgat aaccctcagc
    12001 cttcgttctg ttttgttata cttatgaaaa aactaacatt aggagtgaat aaattggata
    12061 taaatcaaat cttaaaaaaa gagtttaatc ttagagatga acaaataaat aatacattaa
    12121 aacttattga tgaagggaat acaataccat ttatagctag atacagaaaa gaaatgactg
    12181 gtgaaatgag tgatgttaca ctaagagaat tttatgaaaa attgatgtat ttgagaaatc
    12241 ttcaaagtag aaaagatgat gtagttaggc taatagatga acaaggtaaa ttaacagatg
    12301 aaataacaca aaatatagaa aaagcaaaaa cattgcaaga agtagaagat atatatgctc
    12361 catataaaca aaagaaaaga actagagcaa caatagcaaa agaaaaagga ttagaaaact
    12421 tagcattgtc aatactagag aataatttag ataatattga aatagaggca gagaattatc
    12481 ttgatgaaga gaaagaagtt ctttctattg aagatgctct aaaaggtgct agagatataa
    12541 ttgcagaatt agtatcagat gatgctaaga ttagaaagta tataagagaa ttagcattaa
    12601 gagaaggtat gatagttagt aaaagtgcca ctgatgagaa atcggtttat gatatgtatt
    12661 atgattatag tgaagcagta aaaagtatgg ctccacatag agttttggca ataaacagag
    12721 gtgaaaaaga aaatttctta aaggttaagt tagaaataaa taatgataag gttttaaatt
    12781 acataataaa tgaatatgta aatgataaaa atttcaaaaa taaagaagaa attgtaaatt
    12841 caatagaaga ttcctataaa agattaattt ttccatcaat agaaagggaa ataagaaatc
    12901 atttaacaga aatagctcaa gaaagagcta taagtgtgtt tggaaagaat gttaaaagtt
    12961 tattacttca accaccagtt aaagacaaag tagttatggg atttgaccct gcatttagaa
    13021 ctggatgtaa aatagctgta gtagataaaa atggtaaact actagactat acaacagtat
    13081 atccaacaga ccctcaaaat gatgtagaag gagcaaaaaa agttttaaaa ggattgattg
    13141 aaaaatacga tatagatata atttcaattg gaaatggtac tgcatctaga gaatcagaaa
    13201 cttttgtatc agaaatgata aaagagatag atagagaagt gcagtatgtg atagttagtg
    13261 aagccggagc atctgtttat tcagcatctg aacttgcaaa tgaagaacat ccagatataa
    13321 atgtatcaat aagaggagct atatctatag ctagaagatt acaagaccca ttggcagagc
    13381 ttgtaaaaat agaccctaaa agtattggtg tgggtcaata tcagcatgat ttaaataaaa
    13441 agagattaga agaagtacta gatggagttg ttgaagattc tgtaaacagt gttggagtag
    13501 atttaaatac agcatcatac tctctactag agcatattgc aggaataagt aaagctattg
    13561 caaaaaatat aatagcatat agagaagaaa atggagattt tacatcaaga gcacaattga
    13621 aaaaagtaaa aagactagga cctcaagcat ttactcaatg tgctggattt atgagaatct
    13681 tagagggtaa aaatccatta gataatacag gtgtgcatcc tgaaagttat gatatatgta
    13741 aaaaaatgat tgagataatc gggtattctt tagatgatgt aaagaataaa aatatagatg
    13801 aaatagatga aaaaataaaa gaaattggct tgaaagaatt aagtgaaaaa ctagaagtag
    13861 gtcaagttac attaaaagat atcatagctg aaatcaagaa gccaggaaga gaccctagag
    13921 aagaaggtat aaaacctata cttagaactg atgtcttaaa aattgaagat atacaagagg
    13981 gtatgatttt aaaaggtaca ataagaaatg ttgtagattt tggtgcattt gtagatattg
    14041 gaataaaaaa tgacggatta gttcataaat cagaaatgag taatagtttt gtaaaagacc
    14101 ctatgagtat tgttacagta ggtgatatag tagatgtaaa agtcattgga attgacttaa
    14161 acaaaaaaag agttgcctta tcaatgaaaa aatagataag tttatgtatg gttatagtac
    14221 ttaaattcta tgtttgtaaa tgaagttacc agagtgtata aatgttaaaa acttgtaaag
    14281 gtatatagta agaaaataca ttaaaataag aacgatagtc ttgaaaatca ggaggaaaat
    14341 gaatgatttt tataaaaaat ggtaaaataa ataccataac aaatgggata attcatggag
    14401 atatacttat agaggaagga aaaataatag aaatcggaga agatttgata gcacctttag
    14461 atgtagaggt catagatgca agtaataaac tcgtgtttcc tggatttata gatgctcata
    14521 cacatttagg tttatgggaa gatggtatag ggtttgaagg agcagacgga aatgaagaga
    14581 ctgacccaat aacaccacaa ttaaatcctg tagatggaat taatccaatg gataggactt
    14641 ttaaagaagc atttgaaggt ggaataactt ctgtttgtac aactcctgga agtgcaaatg
    14701 ttatgggtgg tcaatgtata gccataaaaa catatggaaa aagaattgat aaaatggtaa
    14761 taaaaaatcc agttgcgtct aaaattgctt ttggggaaaa tccaaagagc tgttacggtc
    14821 aggatgataa atctccccaa actagaatgg caatagctgc cttacttaga gaaaacctaa
    14881 aaaaagcaga agaatatttg gaagatattg atatgtatga gagtcatgat gatgaagact
    14941 gtgaaaaacc tgaatatgat ataaaaatgg aaagtttaat acctgtttta agaagagaaa
    15001 ttccttttaa ggcacatgct catagagcag atgatatgtt cacagcaata agaattgcaa
    15061 aagaatttaa tttaaagctt acattagacc attgtacaga aggtcatctt attgcagatg
    15121 aattagtgga agaagggttt cctgtaatag taggtccatc tttgtcagaa aggtctaaat
    15181 ttgagcttag aaatttgaca tttaatacag caggaatact ttcaaatgca ggtcttgatg
    15241 tatgtataat gacagaccat ccagtaatac cagttcaata tcttccaata tgtgcaggta
    15301 ttgcagtcaa acatggtatg aaagaagaaa aagcaataga atcaattacg ataaatccag
    15361 ctaagacttt aggaatagaa gatagagtag gctctataga agttggtaaa gatgcagacc
    15421 tagtaatttg ggataattct ccactagaga ttcaaagtaa tgtaatttac actataataa
    15481 atggcaaagt agtatatgag aaaaaataac ttgacaatta ttaaaaaatt aactagtata
    15541 agagtgtaag gaaaattaaa aaatcaaaga agatcttcgg ggcagggcga aattccctac
    15601 cggcggtata gcccgcgaac tacttaagtg gttgatttgg tgagattcca aagccgacag
    15661 taaagtctgg atgagagaag atatttatgg aagataaata agtttatttt agtttgttta
    15721 aactatatcc ataaaaactc gaagataggg tcttcgagtt ttttgttttt cctaaaaaat
    15781 ataaacatat tgattagggg gaaataatta tgcaaaacac agccaaaaga ggcaatatga
    15841 tttcaacaag aactttagta gtaatgtcaa ttttatctgc aatatcttat ttattaatgt
    15901 ttattcaagc accaatacca ggtatatttc cagatttctt aaaaattgac ttatcagata
    15961 ttccagcaat atttggagga atgtctctag gaccattagt tggatttgga atagttgtag
    16021 taaaaaatgt gttacaagca ataacagcaa caacaactgg aggaatagga gaatttgcaa
    16081 atatattaat tggtggttct tatgtaatga tactttgtta ttcatataag agaagtggag
    16141 atttaaaagg tgtattgatt ggttttgtat taggaacaat atctatgact ataattggtt
    16201 cattagtaaa ttattttgta atgttgccac tttatggtca acttatggga ctagatgcta
    16261 taataggttt aggttcagct ataaatcctc aagttcatga tttattcact tttgtaattt
    16321 ggatgatagc tccatttaat gtgctgaagg ctgttataat atctttagta acattgccac
    16381 tttataaaaa aatgggaaat ataataaaaa aataatttag acaagaaaat ataaaattaa
    16441 atgtattgaa tagccatagt ttatttataa cttcagtcct atattgtgat tgatatatta
    16501 aatatattat ggctttatta atttatcaaa aaatgtatat aaaataagaa atatttagac
    16561 aaatttaaat tttaaaataa aggaagaatt aaaacgatta actattaaaa tatgaaaaag
    16621 gtatctgcaa tatacagagc gaatttataa taagcaaaaa ataaagttat tattaaaaaa
    16681 atataaagat tagtaatcaa atttttaata ataactaatt taataatgat gggagtgtat
    16741 attcatgaac ttatctaaaa agataaaaaa attattttta gatgataaag ggtattattt
    16801 taatagtaat tggaaagata ttcatattgg tatttatgat acaaaactag gagattcttt
    16861 aaagtatatc actaatgaat atattgaaaa tctggaaagg gtaaaatatg attatcgtga
    16921 attaaaaaat atagaaaatc aaactgatgg tatgtgtatt ttagcaataa ggcagaatta
    16981 caaggcatta gagtatgtga aaagtcaaac tgaagaatta tgtttagaag caataaagta
    17041 taattataaa gcacttgaat atgtaaaaga gcaaacagaa tatatatgtc ttgaagctat
    17101 aaaaaaggat tgtaatgcac tcaagtatgt acgaaataaa acagaagggc ttattataaa
    17161 agtaatacgt cattctacca atatagatgt tgtaagtata ctaaaggctt tagagactca
    17221 aacaagaaga atatgtttag aagcaattaa aaaagatggc aggtgtcttg catacgttag
    17281 agaacagagt gaagaattat gtatagaggc cattaaacaa aattataaag cattaaaata
    17341 tgtagaaaat caaacggaaa aaatgtgtat agaagctgtt aaacaaaatg gtatggcatt
    17401 acaatatgta aataaacaaa cggataaaat atgtatagaa gctgtcaaac aagatggaag
    17461 aagtctacaa tttgtcgata ataagacaga agaaatatgt ataaatgcta taagatattt
    17521 aaataaaaaa tataatatta aagatgtgct gtgttacgta gataattata cagaagatat
    17581 gtgtatagag attgtaagac aaaatggaaa aatgcttatg tatataaaaa atcaaacaga
    17641 aaaaatatgt attgaagcag taaaagagaa ttataagtct cttaagtatg taaaagagca
    17701 atctgagaga atatgtaaag aagcattgaa acaaaattat aaagcaaaag agtatgtaaa
    17761 aattgccatt gatgattgta tataaggtgt atctacaggc atcaataatg aaagataaaa
    17821 aaagataaag acaagtactt tataataatc tgggcttata ctataattaa ttaagcagtg
    17881 atataaattc acatgacgat tatatcaaaa tggagatgaa ttaatggcaa aaatatattt
    17941 agaaaatgaa aataaaacta gagaaatagg atataaatta ggtaaacttt taaaagaagg
    18001 tagtgttatc tgtttagtag gagatttagg agctggcaaa actacaatga cacaaagttt
    18061 ggcagattca ttagggataa aagattatat aacaagtcct acttttacta taataaatga
    18121 atatgaagga aaaatacctt tgtatcattt tgatgtttat agaataggca gtagtgatga
    18181 aatgtatgat attggttatg atgaatatgt aaattcaaat ggaatctgta taatagaatg
    18241 ggctaatcta atagaagaca tacttccaaa agaatatttg aatatagaat tgagatataa
    18301 agatgaagga agagaaatga tattaactcc aaaaggagag ttttataaag aaatagttga
    18361 ggagttgatt aaataatgaa aatactaggt atggatacat cttctatggc agcaagcgta
    18421 gcagttgttg aagatgataa cttaatatgt gaatttacag cgaacaacaa aaaaactcat
    18481 tcgcaaaagc ttatgccaat gatagaaaat atgttgagca tgagtgattt aagtataaaa
    18541 gatatggatt tattagctgt atgtataggt ccaggctcgt ttactggact tagaataggt
    18601 atggccacag taaaagctat ggctcatgta aataatatac caataatagc agtaaattct
    18661 ttagaatctc tagcaaataa catgaatttt tgtgatagaa agatatgttg tatattagat
    18721 gctcagaaaa atcaagttta ttcagctaaa tacaagtttg aaaatggaga gatgatagaa
    18781 ttagatggtg ttgatgttat agagtttgat gctttagtag aggaaattgt atcaactaat
    18841 gaagaattta taatggttgg agaagctgta tacaaatata aagataagct agagaatata
    18901 gaaaatataa agataccttc accagctaat aatgtatcta aagcaggaag tttatgctct
    18961 ttagcattaa ataaatataa taaaaatata gatgtacata cttgttatac aataaatcct
    19021 atgtatatca gaaaatctca agctgaagta caatatgatg aaaaaatgaa gaggctaaat
    19081 aatggaagat aatataaatt taatagtaaa tgatataaaa atagaagaaa tgaccattga
    19141 agatatagat gaagtttttg aagtagaaaa aaattgtttt gaagattatt ggtcaaaaga
    19201 gtcttttaga aaagagttat ctaatgaagt agcaaggtat atagtagcta agttagatgg
    19261 aaaaattgtt ggatatgtgg gaatttggct tatacttgat gaaggacata taaataatgt
    19321 tgcggttcat agtgactata gagggaaaaa aataggggat aaattgataa aaggcatagt
    19381 tgatttatgt aaagataata atattgcatc tatgacatta gaagttagag catcaaataa
    19441 aatagcacaa aatttatata gaaaatatgg atttaaaatg ggtggaataa gaaaagaata
    19501 ctacagtgat aataaagaag atgcaattat aatgtggaac caattaaaag aggtgtagag
    19561 tttatgagtg atataataac attagctata gagagtagtt gtgacgaaac agccgcatca
    19621 gttttaaaaa atggtagaga agtactttca aacataatct ctactcaaat cgagacacat
    19681 aaaaaatttg gtggtgtagt tccagaagtg gcttcaagaa aacatgtgga aaatattgat
    19741 atagttgttc aagaagcttt agacaaagca aatatggatt ttaatgatat agaccatata
    19801 gcagttacat atggacctgg actagtaggt gctttactgg ttggtctatc ttatgctaaa
    19861 gcacttgcct atactttgaa tattccactt gtaggagtaa atcatataga agggcattta
    19921 agtgcaaact atatagagca taaggatttg aaacctcctt ttataacatt aatagtatct
    19981 ggaggtcata ctcacttggt tgaagtaaaa gactatggaa agtatgagat tttaggaaaa
    20041 acaagagatg atgcatctgg agaagccttt gataagattt caagagcaat gaatttaggc
    20101 tatccaggag gtcctattat tgataatcta gcccaaaagg gaaataagca tgctatagaa
    20161 tttccaagag cctatttaga agaagatagt tatgatttta gttttagtgg attaaaatct
    20221 tctgtattaa attatctaaa tggaaagaga atgaagaatg aagaaatagt agttgaagat
    20281 gtagcagcat ctttccaaga ggctgtagta gaggttttat ctactaaagc actaaaagca
    20341 gtaaaagaca aaggatacaa tatcataact ctttctggtg gggttgcttc taattcgggg
    20401 cttagagcta aaataacaga gcttgctaaa gacagtggta ttactgttaa gtatcctcct
    20461 ctaattttat gcacagataa tgcagctatg atagggtgtg caggatatta taattttata
    20521 aacggtaaaa cccatgatat gagtttaaat gcagttccaa atttaaagat aaatcagtag
    20581 agtaagataa atttgtaaat ataaaagtat tgtttcaaaa aagtaagtag aagtaaaatt
    20641 aaatacaata aaaattaata tagtgtatat taaaacttga agtaaaacta tttataatct
    20701 ttatgtccca atgaattgca acacatttta ggatataaag gttgtctttg gcttagattt
    20761 gcttcaagtt taattttttc tgtagaaaga ttattttaaa aagtgggtaa tatacccttt
    20821 tttttcattc ttaaaaagtt ttatactaat tatagaaaga ttaaggaggg gaagaaatga
    20881 gtcaaagtaa agaagacaaa ataagaagta ttttggaagc aaaaaatatt aaatcaaatt
    20941 ttcaaaataa agaaaattta aatgaattta atgaaaaaaa agcaagtaaa agagcagaag
    21001 acttattaga tgtatattat aatacattat ctactgctga tatggaattt ccatattggt
    21061 ataatagaga atatagaaaa tcagatggtg atataccagt agttagaaga gctaaagctc
    21121 ttaaagctgc tttttcacat atgacaccaa atataatccc tggtgaaaaa atagtaatgc
    21181 aaaagacaca ccattataga ggctcttttc caatgccatg ggtaagtgaa agcttctttg
    21241 ttgctcaagg agaacaaatg agagaagagg caaagaaatt ggccagtaac acagctgatg
    21301 agcttacaaa atttgggtct ggcggaggaa atgttactga aagttttgga aatgtcgttt
    21361 caatagctgg taaattcgga atgagaaaag aagaagtacc tgtacttgta aaaatggcaa
    21421 aagaatgggt tggaaagtct gttgaagatt taggttttca ctatgaaaaa atgatgccag
    21481 attatgattt aaaagaaaat ctaatgagca cgcttatatg catgtttgat tcagggtata
    21541 cacttcctca aggtagagaa gttataaatt atttttatcc tttaaattat ggattagatg
    21601 gaattataga aatggcaaaa gaatgtaaaa agtcagttgc aggaaatgca tctggagatg
    21661 ggcttattgg tatggaccgt ctatatttct atgaggctgt tattcaagtt attgaaggat
    21721 tacaaacttg gattttaaat tatgcaaaac atgcaaaata tttagaaagt atagagacag
    21781 atttagaagc taaaaaagaa tattcagatt tagtggaaat attagaacat attgcacata
    21841 aacaacctcg tacatttagg gaagctcttc agttgactta tacaattcat atagcatcag
    21901 taaatgagga tgctatatct ggtatgtcta taggtcgttt cggtcaaatt ttatatcctt
    21961 ggtatgaaca agatatagaa aaaggattaa ttacaaaaga agaggttatc gaattattag
    22021 aactttatag aataaaaatt acttgtattg actgttttgc ttcagcgggc gtaaatggag
    22081 gagtactatc aggaaatacg tttaacactt tatctatagg tggattaaga gaagatggtt
    22141 ctacaggagc taatgagcta gaagaattat tgttagaagc aagtatgaga tgtagaactc
    22201 ctcaacctag tttaactatg ttatatgatg aaaagcttcc agaagatttc ttaatgaaag
    22261 ctgctgaatg tacaaagctt ggttctggtt atccagcatg ggtaaacaat tcaaatggaa
    22321 caacatttat gatgaaacaa tttgctgatg aaggtatgac tgtagaggaa gcaagagcat
    22381 ttgcgttagg tggatgtttg gaaacgtctc cagggtgctg gaaacagtta actttaaatg
    22441 ggaagacata ctctattgca ggtggagcag gtcaatctgc tggttctgga gttcacttta
    22501 tagctaatcc taaaatctta gaacttgtac ttatgaatgg taaagactat cgtatgaata
    22561 tacaagtgtt tgagcctcat aataagccat tagatactta tgaagaagtc atagaagtat
    22621 ttaaagatta ttataaacaa gcaataaatg ttttagaaag agcaaataat attgagttag
    22681 atatatggag aaaatttgat acttctataa taaattcact attaaagcca gattgtctag
    22741 ataaaggtca acatatagga aatatgggat atcgttataa tgcaacatta aacgtagaaa
    22801 cttgtggtac tgttactatg gtcaattcat ttgcagcact taagaaactt gtatatgatg
    22861 acaaagcatt tacgatagag gaaatgaagg atgccatatt aaataatttt ggctttaaag
    22921 atgcattaga agtaggaaat tattctatgg cagaccaagt aaaagtagat aagactggga
    22981 agtatgatgc tatttataag gcgtgtcttg atgcacctaa atatggaaat aatgatttgt
    23041 atgcagacaa tattcttaaa aactatgagg tatggttatc aaaagtatgt gaagaagcac
    23101 aatctttata tgctaagaag atgtatcctt gccaaatatc tgtttctaca catggaccac
    23161 aaggagctgc tactttggct actccagatg gtagattaag tggaacaaca tattcagacg
    23221 gttcagtttc tgcatatgca ggaacagata aaaatggagt atatgcatta tttgaatctg
    23281 caactatttg ggaccaagcg gtagttcaaa attctcagat gaatttaaaa cttcatccaa
    23341 caactataaa aggacaacaa ggaactaaaa aattattaga tttaacaaga agttatttaa
    23401 gaaaaggcgg gttccatatt caatataatg ttgtagattc agaaacatta aaagatgcac
    23461 aaaaaaatcc tgacaattat cgtcaattaa tggtacgtgt tgctgggttt actcaatatt
    23521 ggtgtgaatt aggtaaacca atacaagatg aagtaattgc tagaacagag tatgaagggg
    23581 tgtaaaatat gagaaagcat agtgattgta tgaatttttg tgcagtagat gcaaccaaag
    23641 gaatttgtag attgtcaaaa caaatgatta atttagatga tgaagcatgt ccagagataa
    23701 aagtaatgcc aaaatgtaaa aattgtaaaa attttgttga agctaatgat gaaggtatag
    23761 gcaagtgtgt tggtctagaa aaggaagact gggtatattc aacattgaat gcaattactt
    23821 gtgaaggaca tgtgtttaat gagtagtcaa aagaaattag aaggtatgat ttttgatgta
    23881 caaagctttt cagttcatga tggtccaggt tgtagaacaa ctgtattttt aaatggatgt
    23941 ccactgagtt gtaaatggtg tgcaaatcca gaaagttgga ctgtaagacc acatatgatg
    24001 tttagtgaat tatcttgtca atatgaaaat ggatgtactg tgtgtcatgg taagtgtaaa
    24061 aatggtgcat tgagctttaa tcttgataat aagccagtta tagattggaa catctgtaag
    24121 gactgtgaga gttttgaatg cgtcaactca tgttattata atgcgtttaa attatgtgca
    24181 aaagcttata ctgtagatga acttgtgcag gtaattaaac gtgattctaa caattggaga
    24241 agtaatggag gagtaacatt tagtggaggt gagccactac ttcagcatga atttttacat
    24301 gaagtattat taaagtgtca tgaggtaaat atacacactg ccattgaaac gagtgcatgt
    24361 gtatcaagtg aagtttttaa taaaatattt gaagatattg attttgcatt tattgatata
    24421 aaacatatgg atagagaaaa gcacaaagaa caaacaggtg tgtataatga tttaatacta
    24481 aaaaatatat caaaccttgc aaattctgat tggaatggga gattagtact tagagtacca
    24541 gttatctctg ggtttaatga tagtgttaaa aatataagtg atattatatc ttttatgcac
    24601 aaaaataatt tagtagagat taatctatta ccatttcaca gactaggtga atcaaagtgg
    24661 gttcaattag gtaaagaata tgagtattct gataagggtg atgttgatga aggtcatctt
    24721 gaagaattac aagatatatt tttggataat ggtatagctt gttatgttgg tcatgagaca
    24781 gctttctaaa atcaaataca agttttaatt aaaaaaggat gttgtactaa aagctagagt
    24841 cacatccttt tttatgaggt ttttatgtat aaagagttat aatatatttt actaatttaa
    24901 gataaaaata ataatttttt ataggtggaa tctttgcttg atatagaaat taataataag
    24961 attattccac ttgttccctc taaaaaggat gtgttttcca ttgtttctaa attactaaat
    25021 gttttaaagc tgtatcttaa tgataaatca aacaattgaa atatttcatc taaagttttt
    25081 tcaaggttgt atagataaac attatcattt gtttttttgt acactgatgt cattatagac
    25141 aaaatttaat aactatcctt tatattcttt tgtaaataga aatggaacat aaaaaattaa
    25201 agaaaccata gatactatta atatgtcaga ataagtaata attgactctt caacatttgt
    25261 tataagaaaa gcgctattat ttaaaatatg aattatagca caaaaccaga catttttagt
    25321 tttcatataa agatatccta aaaatattgc aattccaact atatgtattg aacgtaatat
    25381 taatcctaat attggtgttt caggtgtata taatgtaaat attagtggta agtgccacaa
    25441 actccaacac atacctaata ttataactcc tagtttttta ccaaacctat caaaaaaaat
    25501 attctgtaaa aaacctctcc aagcatattc ttcactaaaa aaaatataaa tagtagttaa
    25561 tgatgttatt ggagtaaata taaattttat gatcccgcta taactaggtt gagttccttg
    25621 tagtattaag agaaatgatg ttggaacaat tacagaaatc ataaagtaaa taattaacaa
    25681 agaacctgct ttaatatttt ttacgggata taaatttttt
//
LOCUS       DAFNNB010000038        25539 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0038, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000038 DAFNNB010000000
VERSION     DAFNNB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25539
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0038"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            121..2106
                     /locus_tag="L3M60_001407"
     CDS             121..2106
                     /locus_tag="L3M60_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6226086.1"
                     /translation="MSMTYMDPRVFLILEMVLEEDTNTLEQFSSVLGVSTRTIRNYIN
                     QINRELKDGIAKIVKNEKGTYSIRVEDESKLIEIVNFNRVKLSNFINLNSPDERINYV
                     LDILTMTNNPITIDDLAEEICIGRTTLIKDLKKADRILAEYDLELKRKPNIGMILVGE
                     EMDIRLLVLDRLYENYIDVLENVNQINLVKDYDIECLRDELKKLFKKEELYITEQVLR
                     DVERYIIISALRNLNGYKFEKIDKRFEVIRCSDEYTLGLKLKALLEKIFNIVLNEKET
                     IFLTMPLIGRKAPSTKLALSSIKINDSVKEVVDEVTSFLLETSGIDFKEDKKLIENLE
                     YHLYFALNRMRFNIRVKNPLLQEIKERYTFPYSVAKIAAMIIEEKFDLKICDHEIGYI
                     ALHFGSYFEKNSRKVLNVNRVAVVCGTGLGTAQLIRIKIGKILGESVEVNTFSDIEAK
                     ANLDLLKYYDLVFTTVDFKTDLDVPIFRVNALFNEESIKKEIEDAILLKNSINIKSIK
                     KIEKNIPFIGKLIEEDKFFILNKGTFMENLEEMLEKLVLKGIIDIEFKENVLKREKKS
                     STALDNYIALPHSVSPNEENLYLAFGILENPVIWDTKEIRIIILMIIPDKNVGSTELI
                     IKSYEEVLEIGRDKKIVEKLSKVKSFDEFYKIITTRR"
     gene            2111..2545
                     /locus_tag="L3M60_001408"
     CDS             2111..2545
                     /locus_tag="L3M60_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBN6226087.1"
                     /translation="MDPVIFIIVSFIVVYLLNIFLGYLQIKDFNKNYIELKRKGRVAI
                     GRKKGRISSGAIVLILINDDGVIIETRKMQGVTVVARVKSFEGLVGKNLKYIEKSDLT
                     VYNKLLKKAILDAVKQYITFEKNKCEDMSNLSTDEECRMEVV"
     gene            2574..3134
                     /gene="srlA"
                     /locus_tag="L3M60_001409"
     CDS             2574..3134
                     /gene="srlA"
                     /locus_tag="L3M60_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418408.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBN6226088.1"
                     /translation="MTQFFQVLASGADSFMNLFRAGGQQFVGFVTDIVPLLVALLVTM
                     NAIINFIGTDRVERLAKKCSANIFTRYLILPVLGTFVFANPMTLSLGRFLPEKFKPSY
                     FAAASFSCHTMNGLFPHINPGELFIYLGIANGITTLGFSTADLAVRYLLVGIVANFIK
                     GVVTDYTTKFVEKQQGIALNSDIKIG"
     gene            3198..3566
                     /locus_tag="L3M60_001410"
     CDS             3198..3566
                     /locus_tag="L3M60_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIB"
                     /protein_id="HBN6226089.1"
                     /translation="MSFNKVKIIKGSGGWGGPLIIEPTEKKNKVVYVTGGAKPETAVK
                     IAELTGCELIDGFSQGVKDDEIACVIINCGGTLRCGIYPQKKIPTINIMKTGKSGPLA
                     MFIKEDIYVSGVKPTNVEQL"
     gene            3591..4157
                     /locus_tag="L3M60_001411"
     CDS             3591..4157
                     /locus_tag="L3M60_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBN6226090.1"
                     /translation="MAYDTKKKISEQSQGIVAKIGIALGNVTSTLFQAGRDTIDTLIH
                     TILPFMAFVAVLIGIINQSGIGNWVANLLTPLIGNVGGLVVLSIICSFPLLSPFLGPG
                     AVIAQIVGVLVGTEIGKGNIPPSLALPALFAINSQAACDFIPVGLGLAEAEPDTVQVG
                     VPSVLYSRFLTGAPTVLLAWLASFGLYK"
     gene            4198..4563
                     /locus_tag="L3M60_001412"
     CDS             4198..4563
                     /locus_tag="L3M60_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBN6226091.1"
                     /translation="MELIYSTQINKIGDNANEFLQHNMFVTFKDNAPQELENYCYIHS
                     ENNLVKEILTNDVLSINNVDYKITSVGELVNQNLSELGHITFKFTGEKDGAIGGTLYL
                     EEKEIAPIGVGTILKIIRK"
     gene            4663..5436
                     /gene="srlD"
                     /locus_tag="L3M60_001413"
     CDS             4663..5436
                     /gene="srlD"
                     /locus_tag="L3M60_001413"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HBN6226092.1"
                     /translation="MRKVALVVGGGRSLGEYLSKHLAKVGYDVAIADLNEDNAKKVAK
                     EIEMEFGCKSVGYGCNAIDEIQVMSTIKAIENDFDRIDLLVYNAGVAVSKKITDFGYK
                     DFKFIVDVNLNGYFLFAREVSKVMIAKGIEGRIIQINSKSGRVGSKHNSGYSAGKFAG
                     VGLTQSLALDLAEHKITVNALMLGNLLDSDMFESLIPQYAVKLGIPESEVKQVYIDKV
                     PLKRGCRFMDVANILTFYASKEAEYCTGQSINITGGQVM"
     gene            5784..7892
                     /locus_tag="L3M60_001414"
     CDS             5784..7892
                     /locus_tag="L3M60_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBN6226093.1"
                     /translation="MDITKKLSHGCTLDCHDCCKFNVYTKGNNVVKIEGDKNHPYTKG
                     FICKKGMAHLDRLNHKDRIKTPMLKVDEVWKEISFDKAIEIMAEKLTYYKEKYTSKSV
                     MHYDQYGSGSVLKYIGDIFFNFYGGVSRHKGSPCWSAGMHAQKYDFGVAKSHAIEDML
                     NSKSIFVWGKNPAYTTIHTMQIIKKAKEKGIKIVVIDPIYTKTAQIADKYIQVNPGTD
                     GALAIAMAKIIVEDKLYDEEYINSYVIGFEEYKRYLYSLELSFLIDECGVKENDIREL
                     VALYTNKYSSINVGYGLQKYKNGGNTIRAIDALGAITGQIGFSGGGVNYANKVYPSVI
                     NSDPYNSKLYGQDREFYVSNISKFIEESLRNTSNKINNVSDEPDTKSNKTDYISNDLY
                     SLSGKSIKDNIPIKMAVITKSNMLNQLPDLVELERVFSKIEFKVCFDMFMTDTATLCD
                     LFIPCTNTLESEDIIYSSMTNPYITYNERAVKPAHKLMDEYYFFMELAKKMRLNDYPF
                     VEKSTYLEKVIEPLKCVDKNLDIEKLKNNYFTIHNPVAWEDKKFETPSGKYELYSESI
                     KNLGINPTPVYINNKYKEIEDKNENISFRLLTNHHADTLFSQHFMDKKSIAQAYINQR
                     MAKKVGIKDKDTVILRSKKAKINVQINIDDGVGNYIVKMYVGWWKKHGNPNILTDTGI
                     SDFGGQVTYNESIVEIIRQN"
     gene            7979..8314
                     /gene="spoIIAA"
                     /locus_tag="L3M60_001415"
     CDS             7979..8314
                     /gene="spoIIAA"
                     /locus_tag="L3M60_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HBN6226094.1"
                     /translation="MVNYSLEHKNLYIEFMCSELDHHVANEIREEIDNLLSVNQVKNV
                     VFNFENINFMDSSGIGVIIGRYKKISNEGGRVSVINISSRVKKIFDLSGLNKIIGIYD
                     TYEEALSSL"
     gene            8328..8756
                     /locus_tag="L3M60_001416"
     CDS             8328..8756
                     /locus_tag="L3M60_001416"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HBN6226095.1"
                     /translation="MNNIMEVKFSAISENESLARVIVASFAAKLDPTVDELVDIKTAV
                     SEAVTNAIIHGYEEDSSKFVFLRCEIEGNTIKIIVEDEGYGIENVEKAMEPLYTSKPE
                     LDRSGMGFTVMKSFMDDVEVSSVKDNGTRIEMTKKINVPK"
     gene            8779..9543
                     /gene="sigF"
                     /locus_tag="L3M60_001417"
     CDS             8779..9543
                     /gene="sigF"
                     /locus_tag="L3M60_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigF"
                     /protein_id="HBN6226096.1"
                     /translation="MEVTVAREEKKPLLSHEETLELIEKVQNGDEEAKEILISSNLGL
                     VRSVVSRFLNIGYDREDLFQLGSIGLIKSIYKFDPKFNVKFSTYAVPMILGEIKRYLR
                     DDGMIKVSRSLKQIAVKAKMESEALTKKLGREPSIEELAKAIDVEKEDLVMAMEANFN
                     VEYLQGVIHEEEGSPICLIDKISMKGESEEEKVVDNILLKDILGRLDKRERQIIVLRY
                     FEDMTQSEIGEMLNISQVQVSRIEKKVLSKLKEYIS"
     gene            9830..10267
                     /gene="spoVAC"
                     /locus_tag="L3M60_001418"
     CDS             9830..10267
                     /gene="spoVAC"
                     /locus_tag="L3M60_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HBN6226097.1"
                     /translation="MDKNYKKYVDQISPKPTYLKNYTLAFIVGGIICMIGQAINDLYM
                     KVGGLDKLGASSATSITLIFIGAFLTGLGVYDLIGKRAGAGSIIPITGFANSIVSPAM
                     EYKREGYVLGVGANLFKIAGPVLVYGIGSSILCGIIYYIFKMF"
     gene            10421..11434
                     /gene="spoVAD"
                     /locus_tag="L3M60_001419"
     CDS             10421..11434
                     /gene="spoVAD"
                     /locus_tag="L3M60_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HBN6226098.1"
                     /translation="MKNKRIGKRTVKLENKPTIISTGTIVGPKEGEGPLKDYFDMIMT
                     DDLYGEKTWELAESKMVETASQQAIQKAGKKLTDVNYMLGGDLINQIVPASFAARELA
                     IPFLGIYGACSTMAEGLCIGSMLIDGGFADLVLSGTSSHYCTAERQFRFPLELGNQKP
                     MTAQWTVTGAGSVLLAPNGEGPKVKYVTVGKVIDEGIDDGNNMGPAMAPAAIDTIYSY
                     FEDTNDDPNSFDIIATGDLGTLGKQIAEDFLKEKGIDISKVYTDCGIEMFNLKEQDVH
                     CGGSGCGCSATVFAGYIYDKLRKKEFNKVMLVSTGALLSPTSTLQKQTIPCVAHAVVI
                     VNE"
     gene            11447..11809
                     /gene="spoVAE"
                     /locus_tag="L3M60_001420"
     CDS             11447..11809
                     /gene="spoVAE"
                     /locus_tag="L3M60_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HBN6226099.1"
                     /translation="MLMDYVRVFIVGGIICLIAQVMMDYFKMQTPYVMVTYVTTGVVL
                     TFLGLYEPLVKFAAAGATVPIIGFGYSLATGVIKSIQSDGFLGIFLGGTTATAGGIAA
                     AIFFGYIMSVVFTPKAKP"
     gene            11993..13009
                     /locus_tag="L3M60_001421"
     CDS             11993..13009
                     /locus_tag="L3M60_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN6226100.1"
                     /translation="MVTIKDIARNLGISYSTVSRCLNNNPNVSEKTKKKVVEEANRLG
                     FHFNVNARNLAKKETNRIGVIFSNNFNHQDTRKFFSDIMDSSINSIETNKYDFIIQPN
                     NNISGDSNVYKMVNGQMVDGLVIVSQSIKKEEYDFLKDNNFPHVFIYFKPSFIGEVDN
                     FFWDDNVYGGYIATKHLIDHGHKDIITVTSDDKSSKMHEDRTKGYLNAMEEANLKTEV
                     IKSKMDFESQVEFLEKYIDKIKKASAMFVQQDVPAISIIQELKTTYGINVPEDISIIG
                     YNNIELISYFRSHLSTIDDPREQVIKNGVDSLVNIINKKSTEHIPRKLYPRLIIRNSV
                     KRIK"
     gene            13383..14225
                     /locus_tag="L3M60_001422"
     CDS             13383..14225
                     /locus_tag="L3M60_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBN6226101.1"
                     /translation="MKEKAGIRNAIIAAFIITILAELIGPISFKVMGINVTLLSILWA
                     IFIGMAVSPHLLGRVIPALKKFIGDNEINVSPYLLSLTLYPLGIMFGINAGPKVGILL
                     QAGPALLLQEFGHMGTMLIALPVGVALGLGRSALGGTFSLCRDTALGIIGDKYGLNSP
                     EGIGTLGTYISGSIFGTLLFSFLAPVGVLLGFHPYALAMASGMGSGSMMGAATASLMN
                     TVPQMSEQILAYSATSGLITAVTGIYIELFLSLPVANYYYSKVAPVIERFRGRKTART
                     SNKL"
     gene            14240..14704
                     /locus_tag="L3M60_001423"
     CDS             14240..14704
                     /locus_tag="L3M60_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226102.1"
                     /translation="MEKTFKTIMEQIKIVLFIYIIICFGQMIALKTQAQDLLIGSVSG
                     FLLVIIAIIIKNFVKKPNLPGFAWATLVAFVLSLPISPVGDIIATNVGKINFMSTVTP
                     LLAFAGISVGNQLDILKTLSWKLVVISFIVMTSTYFGSAFISQTVLKLNGML"
     gene            14691..15863
                     /locus_tag="L3M60_001424"
     CDS             14691..15863
                     /locus_tag="L3M60_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBN6226103.1"
                     /translation="MECFKEVVNMNELKVKIKEEIKLLSEEKKTSFEKVSDYLFSNPE
                     LAFEEYKSQKALCDLLEENEFNVTKGVGGLETSFEAVYSNGTNGKTVAFLAEYDALPG
                     MGHACGHNIIGTSSVGAGIILKEIMKKHNIEGTVKVFGTPAEERVGGKITMIKEGVFN
                     NVDAALILHPSDASMPDDISFAQVNLKFDFSGKASHAAAFPWEGKSALSGVIALFNSV
                     NSMRLHLKDYARVHGIITDGGSIHNIIPEKSTAIFNVRALSIEYLNEICEMLKNCAKG
                     AAISTGTSVEIVQLDEIYKEIKNDSELVNIVRQNFEVLGEDYVERDLSQGIGSTDTGN
                     LTHEIPAIQAYIKLKENTATHTDEFAVAAGGEEGRVALIKAIKVLAMCGVDILYSK"
     gene            15907..16842
                     /locus_tag="L3M60_001425"
     CDS             15907..16842
                     /locus_tag="L3M60_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1177 domain-containing protein"
                     /protein_id="HBN6226104.1"
                     /translation="MILKQVIEVYDILDKADANGEEVKTYLQGYGEVDVTVKELSSSK
                     GSTDLVKLTIPGKNGKSKGGDAPTLGILGRLGGIGARPEVIGFVSDGDGALVAIAVAA
                     KLLDMQRKGDILDGDVVISTHICPDAPTKEHYPTPFMDSPIDMMTMNENEVDSSCDAI
                     LSIDTTKGNRVINTRGFAISPTVKEGYILKTSDNLLDIMQTVTGKSPFVFPLSIQDIT
                     PYGNNLYHLNSILQPAVATDAPIVGVAITTEMPVAGCATGATNFSDLDSAGRFAIEVA
                     KLFGRNKCDFYDKEEWEMLIKRYGKLNRFQTFGIQ"
     gene            complement(17468..20161)
                     /locus_tag="L3M60_001426"
     CDS             complement(17468..20161)
                     /locus_tag="L3M60_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBN6226105.1"
                     /translation="MNNDNDKNGNKIRRKKVSSSGNTNKPINRTSANKTRSTKNKKKS
                     KKSDKFKKLRVFGIVFLVLLVVGTAGTAGLVFASLRDVTPVTEAVLDKQTNQTTTIKY
                     ANGKTLSTAPSVNKKTPVPLDKISPYLQHAVIAIEDERFYEHKGVDIKGLFRSVLKTL
                     TGTKQGGSTIPMQVSKMLLTTEQQTIPRKIKDIYYAHEMSKTVSKDKILETYLNNFFV
                     GRGLAGAEAGARGYFDKSAADLTLAESALLAGSTKNPSRFSAYKTSKLEGNETKEDLE
                     NKLLFFVNTTDDDLDDPTQVDFDMIEKIKSWDLISSDTYRQLKAGTLVVRKAVSNPEA
                     KKRQEIVLKKMLDLKYIKQSEYDEAIKAKIEIKLPKSSDKVSSSVEDLIESEVINALM
                     EQGHTNDEAQNLFYNGGLIVNTTIDPKMQDALEEEFDKNSNFPGHMVGPDGVSQPQAA
                     MVILDYKNGEIRALAGGRNISGRKTLNRATNPHQPGSSIKPLAIYTPAIDTLKITQST
                     ALSDSRGGYKFEENNKWNPRTTTAGHGSMSLRKALAKSSNTIAVKTAEMLGDSYDECV
                     DIMMDYLKNFGITTLKNNHSGSSEASDRKFPSLTLGGMANGITPLQMAAAYGTLANQG
                     VYVEPSIFTTITTFDGQLLVKKAPEEHKVVDPEVAYVVTDMLESVITEGTGGVAALPN
                     GMPVAGKTGTTNSAYDAWFVGYTPYYVGATYIGDDAGRKDDSGNTIKRREVPHGSTST
                     AKLWEKVMQKIHANLTVTEFEVPKNVYFTKINLDDGGKQSSGSKAAFIEGTAPTKVSS
                     QPSSEDTKKPDNNQQQENNNNNNNSGDNGGGSTPPDNGGNNGGGSTTPPDNGGNNGGG
                     STTPPDNGGNNGGGSTTPPDNGGNSGGGSTNTPDGGGTPAT"
     gene            20376..21074
                     /gene="yunB"
                     /locus_tag="L3M60_001427"
     CDS             20376..21074
                     /gene="yunB"
                     /locus_tag="L3M60_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HBN6226106.1"
                     /translation="MNRIVDRKTKNEFRKIIAIFLVILFLSVFIGSFIYIDKTLRPTI
                     TVLAETKALELANRSINKAVAEMVEGKINYEDLMDIQLDNNGKITMIQANTIMMNEIA
                     SAIALEIQDELKKDKTASSYIPIGTALGSPILAKYGPKLEVSIEPIGTVSVNFKTEFE
                     SSGINQTRHRIYLEAQTQVKVVIPLITSTKQIKAQIPICETIIVGDVPESYVNIPEKN
                     LGNVLPNTGKNTNK"
     gene            complement(21286..22293)
                     /locus_tag="L3M60_001428"
     CDS             complement(21286..22293)
                     /locus_tag="L3M60_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S55"
                     /protein_id="HBN6226107.1"
                     /translation="MQLKNFHFKDNTLSKKTNLAKSKFTIVLTFLFLLYFFSNNLIYA
                     QNLQKNEPEYLIPIGNVLQIDAELKNIIVRNPGEASPFRLGDAIVKVNNAEVDGYSDF
                     ANTLNALPEEKQVSILLNRGGQLITMKTTKHILEKISFNNLLSGFATLTYINPDTSEF
                     GAVGHPISVGNARKIPIKTGSISTTKHLNIEKSYKGSVGCINAKRENIIGSFTENTDF
                     GIRGQINNFDISKLKKYKVASLDEVKLGKAQIILQDNFNECKKYNIEIIDIENQKHPE
                     SKSFKIKITDKKLLTQTGGIVQGMSGTPIVQDNKIIGAVSHAIENDPAVGYGVFIKWM
                     L"
     gene            22521..23405
                     /locus_tag="L3M60_001429"
     CDS             22521..23405
                     /locus_tag="L3M60_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6226108.1"
                     /translation="MKKYRLLVTCIIAMGIIIIGTSNPVSNFYKTTNLISSGNITNEK
                     ADKGENSNKNNKDKEEDKSNSKSNKDKLSEDKSSDNKNEIQKNKKLLICIDPGHQGKG
                     DSNLEPVAPGSSSKKARVSSGTEGIATKKPEYVLNLEASLVLKNILESKGYNVIMTRE
                     THDVNISNSERAILANDKKADMVVRIHADSLNNSSKTGASILIPEKDGKYTAPIYEES
                     NKCAEFIKQNMEQSGIQINGIIQRGDLTGFNWSKVPAVLVEMGFMSNYNEDQMMSNPD
                     YQRKMMQCIADGLDEYFK"
     gene            23607..25157
                     /locus_tag="L3M60_001430"
     CDS             23607..25157
                     /locus_tag="L3M60_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBN6226109.1"
                     /translation="MLVVENVSHGFGARTILENVSFRLRKGEHIALVGANGEGKSSFL
                     NIITKKLMPDAGNIKWSSRATVGYLDQHTVLSKGKTIREVLREAFKHMFDLEQEMIAM
                     YDKMGEASDDEMSKLLEETAEIQTILENSGFYMIDAKIQEVANGLGLGEIGLDKDVTD
                     LSGGQRTKVLLTKLLLENPTILILDEPTNYLDEEHITWLTKYLQDYENSFVLVSHDIE
                     FINNTCNVIYHMENGELNRYKGNYDEFVRLNDIKKRQEEQAYDKQVEERKRLEDFVAR
                     NKARVATRGMANSRQKQLDKMEILERPKEKIKPTFAFKDARAASKIIFETENLVLGYD
                     EALTKPLNFHLERGKKIALKGMNGIGKSTLLKTLLGIIKPFEGNVKLGDYLEVGYFEQ
                     ESSRENSNTPMDEVWSEFPGLTNFEVRQALAKCGLTNEHITSQMRVLSGGEAAKVRLC
                     KVMLKNINFLVLDEPTNHLDVEAKDELKKAIKEFKGTVLLVCHEPEFYSEIVDDVWNI
                     EDFTTKIV"
     gene            25185..>25539
                     /locus_tag="L3M60_001431"
     CDS             25185..>25539
                     /locus_tag="L3M60_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1461 domain-containing protein"
                     /protein_id="HBN6226110.1"
                     /translation="MKKFLNVLFSVCISIVIIVGVINFTVGFKKLYYFDIDYLNISEL
                     SGLSKDDIKLNYDYLIDYNLNKNVSEFNLPTLKSSPQGKIHFEEVRNIFQNINKLAKL
                     LLIVSLVGIILSVKNK"
BASE COUNT         9743 a         2920 c         4537 g         8339 t
ORIGIN      
        1 tattgtgaaa aagtgtatat tagttcttct ttttcaaata gtatttgaaa aagagaatat
       61 taattatcta tatagtttag attataataa aaaatgtaag ataacaaagg ggagaaaaag
      121 atgtcaatga cttatatgga ccctagggtt tttttgattt tagaaatggt gttggaagaa
      181 gatacaaata ctttagaaca attttcaagt gttctaggtg tcagtactag gactatcaga
      241 aactatataa accagattaa tcgagaatta aaagatggta ttgccaaaat agttaaaaat
      301 gaaaaaggta catatagtat aagagttgaa gatgaaagta aattaattga gatagtaaat
      361 tttaatagag taaagctgtc aaattttata aatttaaatt cacctgatga aagaataaat
      421 tatgttcttg atattttaac tatgactaac aatccaatta ctatagatga tttggctgaa
      481 gagatatgta taggaagaac aacattaata aaggatttaa aaaaagcaga cagaatttta
      541 gctgaatacg atttagaatt aaaaaggaaa ccaaatatag gtatgatttt agttggtgag
      601 gaaatggata taaggttgct agtccttgac agattgtatg aaaattatat tgatgttttg
      661 gaaaacgtta atcaaataaa tttagttaaa gattatgata ttgaatgtct tagagatgaa
      721 ttaaaaaagt tatttaaaaa agaagaacta tacataacag aacaggtatt gagagatgtt
      781 gaaagatata taataatatc tgctttaaga aatctaaatg gttataaatt tgaaaagata
      841 gataagagat ttgaagtaat aagatgttca gatgaataca ctttaggatt aaaactcaaa
      901 gctttattgg aaaagatatt caatatagtt ttaaatgaaa aagaaaccat atttttgaca
      961 atgccattaa taggtagaaa agctcctagt acaaagttag cacttagcag tataaagata
     1021 aatgatagtg taaaggaagt tgtagatgaa gtaactagtt ttttattaga gacctcagga
     1081 attgatttta aagaagataa aaagcttata gaaaatttag aatatcactt gtatttcgca
     1141 cttaatagaa tgagattcaa catacgagta aaaaatccac ttttacaaga aataaaggaa
     1201 agatatacat ttccatatag tgttgcaaag atagctgcaa tgataattga ggaaaagttt
     1261 gacttgaaaa tatgtgacca cgaaatagga tatatagcat tgcattttgg aagttatttt
     1321 gaaaaaaatt caagaaaagt tctaaatgta aatagggtag ctgttgtttg tggaacaggt
     1381 cttgggactg cacaactcat tagaataaag ataggaaaaa tacttggaga gagtgtagaa
     1441 gtaaatactt tttctgatat agaagctaaa gctaatttag atttattaaa gtactatgat
     1501 ttagtattta caacagtaga tttcaagaca gacttagatg ttccaatttt tagagtaaat
     1561 gcgttattta atgaagaaag tataaagaaa gagatagaag atgcaatact tttgaaaaat
     1621 agtataaata ttaagtctat aaaaaagatt gaaaagaata ttccttttat aggtaaatta
     1681 attgaagagg ataagttctt tatattgaat aagggtacat tcatggaaaa tttagaagag
     1741 atgttagaga agctagttct taaaggaatt attgatatag agtttaaaga aaatgtacta
     1801 aaaagagaaa agaaatcttc aacagcatta gataattata ttgcattgcc tcactcagtt
     1861 agtcctaatg aagagaacct ttatttggca tttggaatat tagaaaatcc agtaatttgg
     1921 gatacaaagg aaattagaat aataatattg atgataattc cggacaagaa tgtgggtagt
     1981 acagaactga taataaaaag ctatgaagaa gtacttgaaa taggaagaga taaaaaaata
     2041 gtagaaaaac tttctaaagt aaaatcattt gatgaatttt acaaaataat taccacgagg
     2101 aggtaataat atggacccag taatttttat tatagtatca tttattgtag tttatttatt
     2161 gaatattttc cttggatatt tacagattaa agattttaat aagaattata ttgaactaaa
     2221 aagaaaagga agagtggcaa taggcagaaa gaaaggaaga atttcatcag gtgcaattgt
     2281 gttgatactt ataaatgatg atggtgtgat tatagagact agaaaaatgc agggtgttac
     2341 agtagtagca agagtaaagt catttgaagg tttggtagga aaaaacttaa agtacataga
     2401 aaaatcagat ttaactgttt acaataaact acttaaaaaa gcaatactag atgcagtcaa
     2461 acagtacata acatttgaga agaataaatg tgaagatatg agtaatctta gtactgatga
     2521 agaatgtagg atggaagtag tttaaaaaaa tatatctata aggaggagtt aatatgacac
     2581 aattttttca agtattagcg agtggtgcag atagttttat gaatttattt agggcaggtg
     2641 gacaacaatt tgtaggtttt gtaacagata tagtaccact tttagtagca ttattagtta
     2701 caatgaatgc aattatcaac tttattggta cagatagagt cgagaggctt gcaaaaaaat
     2761 gttctgcaaa tatatttact agatatttga tactaccagt tcttggtaca tttgtatttg
     2821 caaatccaat gacactatca ttgggaagat tcttaccaga aaagtttaaa cctagttact
     2881 ttgcagcagc aagtttttca tgccatacaa tgaatggatt atttccacat attaatccag
     2941 gtgaactatt tatctatcta ggaattgcaa atggtataac aactttggga ttctcaacag
     3001 cagatttagc agttagatat ttgttagtgg gtatagttgc aaattttatt aaaggtgtag
     3061 ttactgacta tacaacaaaa tttgtagaga agcaacaagg aatagcatta aattcagata
     3121 taaagattgg gtaattactt taaggtataa gtaaactact taaataaaaa gatagtgtat
     3181 agaggaggag aaattatatg tcatttaaca aagttaaaat aataaagggt tctggaggat
     3241 ggggtggacc attgataata gaaccaacag aaaagaagaa taaggtagta tatgtgactg
     3301 ggggagcaaa gccagaaact gcagtaaaaa tagctgaact tacaggatgt gagttaatag
     3361 atggattttc acaaggtgtt aaagatgatg aaatcgcatg tgtaataata aattgtggtg
     3421 gaacattgag atgtggtata tatcctcaaa agaaaatacc gactataaat ataatgaaaa
     3481 caggaaaaag tggaccatta gcaatgttta taaaagaaga catatatgtt tcaggagtaa
     3541 aaccaacaaa tgtagagcag ttataattta gatagtagga ggaaagaaat atggcttatg
     3601 atactaaaaa gaagataagt gaacagagtc aaggaattgt tgcaaaaata ggtatagcac
     3661 taggtaacgt gacaagcact ctttttcaag ctggtaggga tactatagat acattaatac
     3721 atactatact gccttttatg gctttcgttg cagtccttat aggcataata aatcaatcag
     3781 gtataggaaa ttgggtagcc aatttactta ctccattaat cggaaatgtt ggaggtttag
     3841 ttgttttatc aataatatgt tcatttccgt tgttgtctcc atttcttgga ccaggagctg
     3901 ttatagcaca gatagtgggt gtattggttg gtacagaaat aggaaaagga aatattccgc
     3961 caagtttagc attaccagca ttgtttgcaa taaactcaca agcagcctgt gactttatac
     4021 cagtaggtct tggtcttgca gaagctgaac ctgatacagt tcaagtagga gttccatcag
     4081 ttttatattc tagattttta acaggtgcgc caaccgttct attagcatgg ttagcaagct
     4141 ttgggttata taaataaaag gatactttat aaataattag agagaggttc aattgatatg
     4201 gaattaatat acagtactca aataaataaa ataggagata atgcaaatga atttttacaa
     4261 cataatatgt ttgtaacttt caaagataat gctcctcaag aattagaaaa ttactgttat
     4321 atacattctg aaaataattt agtaaaagaa atacttacga atgatgtatt atcaataaat
     4381 aatgttgatt ataaaattac ttctgttggt gagttagtaa accaaaatct ttcagaatta
     4441 ggtcatataa catttaagtt tacaggtgaa aaagatggag caattggagg gaccttatat
     4501 cttgaagaaa aagaaatagc acctattggc gtaggtacta tactaaaaat aattagaaaa
     4561 taaaatttaa aacgaatcta atcatattat aagtaaatta tagataaaag tcaatttaac
     4621 taatcaagtt aacttttaat atgatacaaa ggggatatga aaatgagaaa agtagcttta
     4681 gtcgttggag gaggaagaag tttaggtgaa tatttatcta aacatttagc taaagtaggt
     4741 tatgatgttg ctattgcaga tttaaatgaa gataatgcaa aaaaagtagc taaagaaata
     4801 gaaatggaat ttggttgtaa atctgtagga tatggatgta atgctataga tgagatacag
     4861 gttatgagta ctatcaaagc tattgaaaat gattttgata gaatagattt attagtgtac
     4921 aatgcaggag tggctgtaag taaaaaaata acagattttg gatacaaaga ttttaaattt
     4981 attgtggatg taaatttaaa tgggtatttt ctatttgcaa gagaagtgtc taaagttatg
     5041 atagcaaaag gtattgaggg tagaataata cagataaatt caaaatcagg tagagtagga
     5101 agcaaacata attcaggtta ttcagcaggt aaatttgctg gagtaggact tactcaaagt
     5161 ttagcactag atttggcaga acataagata actgtaaatg cactgatgct tggtaatttg
     5221 cttgattctg acatgtttga aagtttaatt cctcaatatg cagtaaaatt gggaatacct
     5281 gaatcagaag ttaagcaagt ttatatagat aaagtacctt taaaaagagg gtgtagattt
     5341 atggatgttg caaatatact tacattttat gcttcaaagg aagctgaata ctgtactggt
     5401 caatctataa atattactgg tggacaagtt atgtaaaatt tttatgggag tgtctcaaga
     5461 tgaacttttt agtttatgag acactttttt tgtataaaat taaatatatt tttagcattt
     5521 gaataaaaaa gaggcttatc tatattttga atcaacctca tttttgtaat taagaaatat
     5581 ggtatttaag taagcatata agatttttag ttttttagaa gagaaatttt cactaatatt
     5641 atatttatga agtattttta aacttaattt gataaaatat atctatgttt tgtatactgt
     5701 agttttgaac atataaggtt taataaatta tagtatatat gttaaatttt aataatgtaa
     5761 gaaaatagaa agaaggatat tttatggata taacaaaaaa attaagtcat ggatgtacac
     5821 tagattgcca tgattgttgt aaattcaatg tctatactaa aggaaataat gtagtaaaaa
     5881 ttgaaggaga taaaaatcat ccttacacaa aaggttttat atgcaaaaaa gggatggctc
     5941 atttagatag attaaatcat aaagatagaa taaagactcc tatgcttaaa gttgatgaag
     6001 tatggaaaga gatttctttt gataaggcta tagaaataat ggcagaaaaa cttacatact
     6061 ataaagaaaa gtatacatca aagtctgtca tgcattatga ccaatatgga agtgggtctg
     6121 tactaaaata cataggagat atatttttta atttttatgg aggtgtcagt aggcataagg
     6181 gaagtccatg ttggagtgca gggatgcatg ctcaaaaata tgattttgga gtagctaaga
     6241 gccatgcgat agaagacatg ttaaacagta aaagtatatt tgtgtgggga aaaaatccag
     6301 catacactac aattcatact atgcaaataa ttaaaaaagc taaagaaaaa ggtataaaga
     6361 tagtagtgat tgacccgata tatacgaaaa cagctcaaat agcagataag tatatacagg
     6421 taaatcctgg aacagatgga gctttagcta tagctatggc aaaaattata gtagaagata
     6481 aattatatga tgaagaatat ataaactcat acgttattgg ttttgaagag tataaaagat
     6541 atttatattc gttggaatta agttttttga ttgatgaatg tggtgttaaa gagaatgata
     6601 taagagagtt agttgcttta tatactaaca aatattcgag tataaatgtt ggatatgggc
     6661 ttcaaaaata taaaaatggt ggaaacacaa taagagctat agatgcttta ggagctataa
     6721 ctggtcaaat aggatttagt ggtggagggg taaactatgc taataaagtt tacccaagtg
     6781 ttataaattc tgacccttac aatagtaaat tatatggtca agatagggag ttttatgtaa
     6841 gtaatatcag taaattcata gaagaatctt taagaaatac atcaaataaa ataaataatg
     6901 tatcagatga acctgataca aaatcaaata aaacagatta tatatcaaat gacttatata
     6961 gcttatctgg taagtcaatt aaagataata ttcctataaa gatggctgtg ataactaaaa
     7021 gcaatatgct caatcaactg ccagatttag tagaattgga gagggtgttt tcaaaaattg
     7081 aatttaaagt atgttttgat atgtttatga ctgatacagc cactctatgt gacctgttta
     7141 taccttgtac aaatacacta gagagtgaag atataattta tagttcaatg actaatccat
     7201 atataaccta taatgaaaga gctgtaaagc cagcacataa attgatggat gaatattact
     7261 tctttatgga gctagccaaa aagatgagat taaatgatta tccatttgta gaaaagagca
     7321 cttatttaga aaaagtgatt gaaccactta agtgtgttga caaaaatctg gatatagaaa
     7381 aacttaaaaa caattacttt actatacata accctgttgc ttgggaagat aagaagtttg
     7441 agactccttc tggaaaatac gaactttatt cagaaagtat aaaaaacttg ggaataaatc
     7501 caacacctgt atatataaac aataaatata aagagataga agataaaaat gaaaatattt
     7561 cttttagatt attaactaac catcatgcgg atactttatt tagtcagcat tttatggata
     7621 aaaaatctat agctcaagca tatataaatc aaagaatggc taaaaaagta ggtatcaagg
     7681 ataaagatac tgtgatattg aggtcaaaaa aagcaaaaat aaatgtacaa ataaatatag
     7741 atgatggagt aggtaattat atagtaaaaa tgtatgttgg atggtggaag aaacatggaa
     7801 atcctaatat tttgacagac actggtatat cagattttgg tggtcaggta acatataatg
     7861 aaagtatagt agaaataata cgacaaaact agttaaattt cattgaagga ataaaaatat
     7921 aattatagaa ttgattaaaa gttactattt ttacaaaact attgaaggag ggattttgat
     7981 ggtaaattat tccttagaac ataagaatct atacatagaa tttatgtgct cagaactaga
     8041 ccaccatgta gccaatgaaa taagggaaga aatagataac ttattaagtg taaatcaagt
     8101 aaaaaatgtt gtatttaatt ttgaaaatat taattttatg gattcttcag gtataggtgt
     8161 catcataggt agatataaaa agatttctaa tgaaggggga cgagtttcag taattaatat
     8221 aagttcacga gttaagaaga tttttgattt atctgggtta aataaaatta ttggcattta
     8281 tgatacgtat gaagaagctc taagttcttt gtagggagga gtatgtgatg aataatatta
     8341 tggaagttaa attttcggca atatctgaaa atgaaagttt agcaagagtt atagtagcat
     8401 cctttgcagc taagttagac ccaacagtag atgagttagt ggatataaaa actgctgtct
     8461 ctgaggctgt tacaaatgct ataatacatg gatatgaaga agacagttct aagtttgttt
     8521 tcttaagatg tgagatagag ggaaatacta taaagattat agttgaagat gaagggtatg
     8581 gaatagaaaa tgtggagaag gcaatggaac cactttatac atcaaaacca gaacttgata
     8641 gatcaggaat gggattcaca gtaatgaaaa gctttatgga cgatgtagag gtaagttctg
     8701 taaaagataa tgggactaga attgaaatga ctaaaaaaat aaatgtgcct aaataaagtc
     8761 caaaagtgag gttttattat ggaagtaact gttgccagag aagaaaaaaa acctcttctt
     8821 agtcatgaag agactctaga acttattgaa aaagttcaaa atggagatga agaggcaaaa
     8881 gaaatattaa tatcaagcaa tttaggtcta gttaggagcg ttgtatctag atttttaaat
     8941 ataggatatg atagagaaga tttatttcag ttaggttcta taggcttaat aaaatcaata
     9001 tataaatttg acccaaaatt taatgtaaaa ttctctactt atgcagtacc aatgatttta
     9061 ggtgagataa agagatactt aagagatgat ggtatgataa aagtgtcaag gtcgttaaag
     9121 caaatagctg taaaggcaaa gatggaaagt gaagctttaa ctaaaaaatt gggtagagaa
     9181 ccgagcatag aagaattagc taaagcaata gatgtagaaa aagaagattt agttatggct
     9241 atggaagcta atttcaatgt tgagtattta caaggagtta ttcatgaaga ggaaggctct
     9301 ccaatatgtc ttattgataa aataagcatg aagggagaaa gtgaagaaga gaaagtagtg
     9361 gacaacatat tgttaaaaga catacttgga agattggata agagagaacg tcaaataata
     9421 gttctcagat attttgaaga tatgactcaa agtgaaatag gagagatgct taatatatca
     9481 caagttcaag tatctaggat agagaagaaa gtattgtcta agttaaaaga atacatatca
     9541 tgacaatcct tatgatatgt agaataacaa aatataataa tataataaat gtattactaa
     9601 aataaaatgt aataattttt tatgttaagt ttttaaatta ttaaaatata ttaaaataat
     9661 aaagagtata aaaaaataaa aatattaaaa caaggtgtct ttatgggaaa atagattttt
     9721 tctttattag gcatcttttt tattttttga ctaaacttta gatagtcaaa ataaataata
     9781 ggtttatttt tttaaaatta acaaataata ctttagagag gtgtttagta tggataaaaa
     9841 ttataaaaaa tatgtagacc aaataagccc aaaacctaca tatttaaaaa actatacact
     9901 tgcatttatt gtaggtggaa ttatttgtat gatagggcaa gcaataaatg atttgtacat
     9961 gaaagttgga ggtcttgata aattaggtgc cagttctgca acatcaataa cattaatatt
    10021 tataggggca tttttaactg gattaggagt ctacgaccta ataggaaaaa gggctggagc
    10081 tggttctata attccaataa caggttttgc taactcaata gtatctccag caatggagta
    10141 taaaagagaa ggctatgtat taggtgtagg tgcaaatcta tttaaaattg caggaccagt
    10201 tttagtttat ggtataggtt cttctatttt atgtggaatt atttattata tttttaagat
    10261 gttttagatg atttttattt aatttaaatt ttattaatta tgaagaatgt ataaaatctg
    10321 aaaatagtta ctaaagtagc tagttgagag ataatcatgc atatttaatt aatattaaaa
    10381 aattaaatag taaaatgcaa aattaaaaag gtgtgaaaat atgaaaaata aaagaattgg
    10441 aaaaagaaca gtcaagttag aaaataaacc aacaataata tcaactggaa ctatagtagg
    10501 tcctaaggaa ggtgaaggac cactaaaaga ctattttgat atgattatga ccgacgattt
    10561 atatggtgag aagacttggg aattagctga aagtaaaatg gtagagactg catctcaaca
    10621 agctatacaa aaggcaggta aaaaattgac agatgtaaat tatatgctag gtggagattt
    10681 aataaatcaa atagtgccag catcatttgc agcaagagaa ctggccattc catttttagg
    10741 tatatatgga gcttgttcta caatggcaga aggtctttgt ataggttcta tgcttataga
    10801 tggtggtttt gcagacttag tactgtcagg gacatcaagt cattattgta cagcagaaag
    10861 acagtttaga ttcccgctag aacttggaaa tcaaaaacca atgacagcac agtggacagt
    10921 tacaggagca ggaagtgtat tactagctcc taatggagaa ggaccaaagg taaaatatgt
    10981 tacagttggt aaagtaatag atgagggcat tgatgatggc aataatatgg gaccagctat
    11041 ggctccagca gcgatagata ctatctactc ttattttgaa gatactaatg atgacccaaa
    11101 tagctttgat ataattgcta ctggagattt aggtactctt ggaaaacaga tagcagaaga
    11161 ttttttaaaa gaaaaaggta tagatatatc gaaagtttat actgactgtg gtatagagat
    11221 gtttaatctt aaagagcaag atgtccattg tggtggaagt ggatgtggat gttcagctac
    11281 agtatttgca ggatatatat atgataagtt gagaaaaaaa gaatttaaca aagtaatgct
    11341 cgtatcaaca ggagcactac tttcaccaac aagtacacta caaaagcaga caataccatg
    11401 tgttgctcat gcagtagtaa tagtaaatga gtaggaagtg atatatatgt taatggacta
    11461 tgtaagagta tttattgtag gtggaattat atgtttaata gcccaagtaa tgatggatta
    11521 ttttaaaatg caaactccat atgttatggt aacgtatgta acaactggtg tagttttaac
    11581 ttttttaggg ctttatgagc cattagttaa gtttgcagca gcaggtgcaa cagttcctat
    11641 aataggattt ggatattcac ttgctacagg agtaataaaa tcaatacaga gtgatggttt
    11701 tttaggcatt tttctaggag gtacaactgc tacggctggt ggaatagcag cagccatatt
    11761 ctttggatat attatgtcag tcgtatttac tccaaaagca aaaccataaa tctttattta
    11821 agagaataat aacttttaga aataataact ttttataaag taggtggata tcttatagat
    11881 tccacctatt ttttatataa agaagcgttt acataattga agttgacaat tatttacaac
    11941 atagtgtagt attatattat caaaataaaa tgaagtgaag taggggataa agatggtaac
    12001 tattaaagat attgcaagga atctaggtat cagttattca accgtttcta ggtgtttaaa
    12061 caataatcct aatgtatctg aaaaaacaaa aaagaaagta gtagaagagg caaatagact
    12121 agggttccat tttaatgtaa atgcaagaaa tctggcaaaa aaagaaacca acagaatagg
    12181 ggtaatcttt tcaaataatt ttaatcatca agatactaga aaatttttta gtgatataat
    12241 ggacagctct ataaattcta ttgagactaa taaatacgat tttataatac aaccgaataa
    12301 taacattagt ggtgatagta atgtatataa aatggtaaat ggacaaatgg ttgacggatt
    12361 agtaattgtg tctcaatcaa taaaaaaaga agagtatgat tttttaaaag acaataattt
    12421 tccacatgta tttatatatt ttaaaccatc ttttatagga gaagtagaca atttcttttg
    12481 ggacgataac gtatatggag gttatatagc taccaaacat ttaattgacc atggtcataa
    12541 agatataata acagtaactt cagatgataa gagttcaaaa atgcatgaag atagaaccaa
    12601 gggatatttg aatgctatgg aagaggcaaa tttaaagact gaagtcatta aatctaaaat
    12661 ggattttgaa tctcaagttg aatttttaga aaaatatatt gataaaataa aaaaagcttc
    12721 agctatgttt gtacaacaag atgtaccagc gatttctata atacaagaat taaaaactac
    12781 ttatggtata aatgtaccag aagatatttc tataataggg tataataata ttgaattaat
    12841 atcatacttc aggtcgcatt tatcaacaat agatgaccca agagagcaag ttataaaaaa
    12901 tggagtagac agtttagtaa atataataaa taaaaaaagc acagagcata ttccaagaaa
    12961 actatatcca aggttaataa tccgaaatag tgttaaaaga ataaaatagt aagtttttaa
    13021 gcattaaaat tttataaaaa atattataga ttatgtgaag tgtttttaca aataatataa
    13081 ataaaattaa aaggagtagg tgtatataag tacattgtac tcctttattt attgagagaa
    13141 tttttataat atgaatgaat aattttattc aattatttga aaatctatta ttttaataat
    13201 gcaaattaat aaattgtttg cattttatat catgaatgga taatattttt ttgattcata
    13261 actaaaagta tataaaaatg atgaataaat atatattata cattatcaat gataggttat
    13321 ataataaaat cactaacaat tatattataa gaaaattata aaaattacaa ggaggtatag
    13381 aaatgaaaga aaaagcaggg attagaaatg ccataatagc agcctttata atcacaatat
    13441 tggcagaatt aattggacct atttcattta aagttatggg aattaatgta actttactta
    13501 gtatactatg ggctatattc ataggaatgg ctgtttctcc tcatttatta gggagagtta
    13561 taccagcctt aaaaaaattt ataggggata atgaaataaa tgtatctcca tatttattgt
    13621 ctttaacact atatccatta ggaataatgt ttggaattaa tgcagggcct aaagttggta
    13681 tattacttca agcaggacca gcacttttat tacaagaatt tggtcatatg ggtacaatgt
    13741 tgatagcatt acctgttgga gtagcattag gtcttggaag aagtgcatta ggtggtactt
    13801 tttcattatg tcgtgataca gcgctaggaa ttattggaga taagtatgga ttaaattcac
    13861 cagaagggat aggaacacta ggaacttata taagtggtag tatatttgga acattattat
    13921 tttcattttt agcaccagtg ggtgtgcttt taggatttca tccatatgct ttagcaatgg
    13981 catcaggaat gggtagtggt tcaatgatgg gagctgcaac agcatcatta atgaatacag
    14041 ttccacaaat gtcagagcag attttagcat attcagcgac tagtggattg attacagctg
    14101 ttactggaat atatatagaa ttatttttat ctttaccagt agccaactac tattatagca
    14161 aagtagctcc agttattgaa agatttagag gaagaaaaac agcaagaaca agtaataaat
    14221 tgtaaaggag gatatatata tggaaaaaac ttttaagacc attatggaac agataaaaat
    14281 agtattgttt atatatatta ttatttgctt tggacaaatg atagcactta agactcaggc
    14341 tcaggattta ttgattggaa gtgtatctgg atttttgttg gtgataattg ctattataat
    14401 aaagaatttt gttaaaaaac ctaatttacc aggttttgca tgggctacat tagttgcatt
    14461 tgtattatca cttccaatat ctccagtggg ggatatcata gcaacgaatg taggaaaaat
    14521 taattttatg tctacagtaa caccattatt agcttttgca ggaatctctg taggtaacca
    14581 attagatata ttaaagactt tgagttggaa gttagtagtt atatcattta tagttatgac
    14641 atctacttac tttggttctg cttttatatc tcaaacagtt ttaaaattaa atggaatgct
    14701 ttaaggaggt agtaaatatg aatgaattaa aagtaaaaat taaagaggaa ataaagttat
    14761 tgtctgaaga aaaaaagaca tcatttgaaa aagtttctga ttatttattt tcaaatccag
    14821 aattagcatt tgaggagtat aagtcacaaa aagcattatg tgatttatta gaagaaaatg
    14881 agtttaatgt aactaaaggt gtaggaggat tagaaacatc ttttgaagct gtttattcaa
    14941 atggaactaa tgggaaaaca gttgcttttt tagctgaata tgatgcactt ccaggaatgg
    15001 gacatgcatg tggacacaac atcataggga caagttctgt tggtgcaggt atcatattaa
    15061 aagaaatcat gaaaaaacat aatatagaag ggactgtaaa agtttttggg acacctgcag
    15121 aagaaagagt aggtggaaaa ataactatga ttaaagaggg tgtatttaat aatgtagatg
    15181 ctgcattaat tttacatcca agtgatgctt caatgcctga tgacatatca tttgctcaag
    15241 ttaatttaaa atttgatttt agcggtaagg catcacatgc tgctgctttt ccttgggaag
    15301 gaaaaagtgc attaagtgga gtaatagcac tatttaatag tgtaaatagt atgagattac
    15361 atcttaaaga ttatgcaaga gtacatggaa ttataactga tggaggtagc atacataata
    15421 taattcctga aaaatctaca gctatattta atgtaagagc tttaagcata gaatatttaa
    15481 atgagatttg tgaaatgctt aaaaattgtg ctaaaggagc tgcaatatct actggaacta
    15541 gtgtggaaat agttcaatta gatgaaatat acaaagagat taagaatgat tctgaattag
    15601 tgaatatagt tagacaaaat tttgaagttt tgggtgaaga ctatgttgaa agagatttaa
    15661 gtcaaggtat aggttcaact gatacaggaa atttaactca tgagatacct gcaatacaag
    15721 cgtatatcaa attaaaagaa aatactgcaa ctcatacaga tgaatttgct gttgcagctg
    15781 gtggggaaga aggtagagta gcacttataa aagcaattaa ggtgctagca atgtgtggag
    15841 tagatatttt gtatagtaaa taatatataa ttttaaaata tattaagaaa ggaaatcaaa
    15901 ttagacatga tattaaaaca agtaatagag gtttatgaca ttttagataa agcagatgct
    15961 aatggggaag aagtaaaaac ttatttacaa ggttatggtg aagtagatgt aactgtaaaa
    16021 gaattgtctt caagtaaagg ctcaacagat ttagtaaagc ttacaattcc tggtaaaaat
    16081 ggaaaatcaa aaggtggaga tgctcctact cttggtattt taggtagact aggagggatt
    16141 ggagcacgac cagaagttat aggttttgtc tcagatggtg atggagcatt ggtagcaatt
    16201 gcagtggctg ctaaactttt ggatatgcaa cgaaaagggg atatattaga tggtgatgtt
    16261 gtaatttcaa ctcatatttg cccagatgct ccaactaaag aacactatcc tactcctttt
    16321 atggattctc caattgatat gatgactatg aatgaaaatg aagttgatag tagttgtgat
    16381 gcaatacttt ctatagatac tactaaagga aatagggtaa tcaatactag aggatttgca
    16441 atatcaccaa ctgttaaaga gggctacata ttaaaaacta gtgataacct tttagatatt
    16501 atgcagactg taactgggaa gtctccattt gtattccctt tatctattca agatataaca
    16561 ccatatggaa acaatctgta tcacttaaat agtattttac aaccagcagt agctacagat
    16621 gcaccaatag taggggtagc aattacaaca gagatgcctg ttgctggatg cgctacaggt
    16681 gcaactaatt ttagtgattt agattcagct ggtagatttg caattgaagt tgccaagcta
    16741 tttggtagaa ataagtgtga tttctatgat aaagaagagt gggaaatgct tattaaaaga
    16801 tatggtaaat taaatagatt tcagacattt ggtatacagt agttagaaaa atgttaagtt
    16861 tatataattt gtgatataaa tatttacaat atataaacaa ctagaaataa aagatatagt
    16921 ttaagactta aaaaataaaa tatttatttg attacataaa taaggagagt tctatttagg
    16981 taaaagccaa taaataagta taacatcaaa actaacttaa tggtttatat gcaggattga
    17041 gacgaaacag tgcgtattgt aggaaaatgg tatgcactgt tttttcgttc tgttatgtat
    17101 tagaacagca tttcaaattc catatctgct aattttatat attgctcata agcgagatac
    17161 ttaccaaatt aattgcaatg ttaataatat cttgttttgt aacaattggt tttggttgca
    17221 tttttctatc cttcttttgg taatttatgt tgttcaacta atattatcgt tgtagcaaag
    17281 acatataaat gtgtcaataa acatcaaaga aaagctacaa acatagagta taatatactt
    17341 tatcagattt ctctttttta tgaaaataca taatataatt taattataaa aaaaggagtg
    17401 tttcaaagtg atttttttta tcactttgag acaactcctt atatttttat tcaaatttta
    17461 ttttttatta agttgctgga gttccaccac catcaggggt attagtagag ccaccaccac
    17521 tatttccacc attatcagga ggagtagtag agccaccacc attgtttccg ccattatcag
    17581 gaggagtagt agagccacca ccattgtttc caccattatc aggaggagta gtagagccac
    17641 caccattatt tccaccatta tcaggaggag tagaaccgcc accattatca ccagagttat
    17701 tattgttatt attattttct tgttgttgat tattatctgg tttttttgta tcttcagatg
    17761 atggttgaga acttacttta gtaggagcag taccttcaat aaatgctgct ttagagccag
    17821 aagattgctt tcctccatca tctaaattaa tttttgtaaa atatacgttt ttaggaactt
    17881 caaattcagt aactgttaaa ttagcatgta ttttttgcat gactttttcc catagttttg
    17941 cagtactagt acttccatgt ggtacttctc tacgttttat agtgttacca gaatcatctt
    18001 ttctaccagc atcatctcca atataagttg caccaacata ataaggagtg taaccgacaa
    18061 accaagcatc atatgcacta tttgtagtac ctgttttacc tgctacaggc attccatttg
    18121 gaagtgctgc aacaccacca gtaccttcag taattacgct ttctaacata tctgtaacaa
    18181 cgtaagctac ttcagggtct acaactttat gttcttcagg agctttttta actaataatt
    18241 gaccatcaaa ggtagttata gtagtaaaaa tagaaggctc tacataaaca ccttgatttg
    18301 ccaatgtacc ataagcagca gccatttgaa gcggagtaat accattcgcc ataccaccta
    18361 gtgtcaatga tgggaatttt ctatcacttg cttcagatga acctgagtga ttatttttta
    18421 aagttgttat accaaagttc tttaagtaat ccatcattat atctacacat tcatcataag
    18481 agtctccaag catttcagca gtcttaacag ctattgtgtt tgaagattta gcaagtgctt
    18541 tacgtaaact catagagcca tgaccagcag tagtagttct tggattccat ttattatttt
    18601 cttcaaactt atatcctcct ctagaatcac ttagagcagt tgattgtgtt atttttagag
    18661 tatcaatagc tggtgtatat attgctaaag gttttataga agaacctggt tgatgagggt
    18721 tagtagcacg atttaaagtt tttctaccac ttatatttct tcctccagct agtgctctta
    18781 tttcaccatt tttataatct aagattacca ttgcagcttg tggttggcta actccatcag
    18841 gaccgaccat atgtcctggg aaattgctat ttttatcaaa ctcttcttct aaagcatctt
    18901 gcattttagg gtctatagtt gtatttacaa ttaaaccacc attatagaat aagttttgag
    18961 cctcatcatt tgtatgtcct tgttccatta aagcatttat aacttcactt tcgataaggt
    19021 cttctacaga tgaagaaact ttatctgatg atttaggcaa ttttatttct attttagctt
    19081 tgattgcttc atcatattca ctttgtttta tatatttcaa gtctaacatt ttctttaaaa
    19141 caatttcttg tcttttctta gcttcaggat tacttacagc ctttctaaca actaatgtac
    19201 ctgcttttag ttgtctataa gtgtcactag atataaggtc ccaagattta attttttcaa
    19261 tcatatcaaa atcaacttga gtagggtcat ctaaatcatc atcagttgtg ttaacaaaaa
    19321 ataataattt attttctaaa tcctccttag tttcatttcc ctctaactta gaagttttat
    19381 aggctgaaaa tcttgatgga tttttagttg aaccagcaag aagtgctgac tctgctagag
    19441 taaggtcagc tgcagactta tcaaaataac cacgtgcacc agcctcagca cctgctagac
    19501 ctctaccaac gaagaagtta tttaaataag tttctagtat tttatcttta cttactgttt
    19561 tactcatctc atgtgcatag taaatatctt ttattttacg aggtatagtt tgttgttcag
    19621 ttgttaacaa catttttgat acctgcatag gaatagtact accaccttgc ttagtacctg
    19681 taagggtctt taatactgac ctaaataaac ctttaatgtc gacaccttta tgttcataaa
    19741 aacgctcatc ttctattgca attacagcat gttgaagata aggggaaatc ttatctaatg
    19801 gaacgggtgt ctttttattt acacttggag ctgtagataa tgtttttcca ttagcgtatt
    19861 taattgtagt tgtttggttt gtctgtttat ctaaaacagc ttctgtaact ggagttacat
    19921 ctcttagtga tgcaaatact agaccagcag tacctgctgt accaacaact agtaaaacta
    19981 aaaatactat cccaaatact cttagttttt taaatttatc gcttttttta gattttttct
    20041 tattttttgt tgaacgagtt ttgttagctg aagttctatt aattggttta tttgtattac
    20101 cagatgagct aacttttttt cttctgattt tatttccatt tttatcatta tcgttattca
    20161 taagattttt aacctcctta cttaatgcaa tattataaca caaataggag tagttttgta
    20221 attaatagaa aaaatgaatt tgctataata ttctgtacat tgtatatgta taaacataat
    20281 atattaaaaa gtctatattt ttattttatt tatttttgac aaatattata aaaattatgc
    20341 caaagctaca tataaataaa taggagggga taattttgaa tagaatagta gatagaaaaa
    20401 caaaaaatga atttagaaaa attatagcta tttttttagt aattcttttc ttgtctgtat
    20461 ttataggcag ttttatatat atagataaaa ctttaagacc aacaataaca gttttagcag
    20521 aaactaaagc tcttgaatta gcaaacaggt ctataaataa agctgttgct gagatggtag
    20581 aaggaaaaat aaattatgaa gatttaatgg atatacagct tgataataat ggcaaaataa
    20641 ctatgataca agcaaatact ataatgatga atgaaattgc atctgcaata gctttagaaa
    20701 tacaagatga attaaaaaag gataagacag caagttctta catacctata ggaacagcat
    20761 taggaagtcc tatattagct aaatacggtc ctaaacttga agtgtctatt gaacctatag
    20821 gaacagtatc agtaaacttt aaaacggaat ttgagtcttc aggtataaat caaactaggc
    20881 atagaatata tttagaagct caaacacagg ttaaagtagt aataccactt ataacttcaa
    20941 caaaacaaat taaagctcaa atacctattt gtgaaactat aatagtaggt gatgttccag
    21001 aaagttatgt aaatattcca gaaaaaaatc taggaaatgt tttacctaac actggaaaaa
    21061 atactaacaa gtaatataaa gttgtatatt tttaaataaa ttcaaaggaa tagtttataa
    21121 ataaaatttt tggaaataat attaatataa ttttagtcaa aatttatatt ttatactatg
    21181 taaaatatct aatatatttg gtctgtaggt taaataaaat tacctttaga ccaaatatat
    21241 tattaataac ttaataacgt cttaaaactt atttatttat attatttaca acatccattt
    21301 aataaataca ccataaccta cagctgggtc attttcaatt gcatgtgata ctgctccgat
    21361 tattttatta tcttgaacta ttggagtacc actcatacct tggacaatac caccagtttg
    21421 agttaaaagc tttttatctg taattttaat tttaaaactt ttactttctg gatgtttttg
    21481 gttttctata tctataattt caatattata ttttttacac tcattaaagt tatcttgtaa
    21541 aattatttga gcttttccta atttaacttc atctaaagat gctactttat atttttttaa
    21601 cttggaaata tcaaagttat taatttgacc tcttattcca aaatcagtat tttctgtaaa
    21661 gctaccaata atattttctc gttttgcatt tatacatcca acactacctt tataagattt
    21721 ttctatatta aggtgcttag ttgtagaaat tgaacctgtt ttaataggta tttttcttgc
    21781 attaccaaca cttattggat gtcctacagc tccaaactct gatgtatctg gattgatata
    21841 cgttaaagtg gcaaatccag aaagtaaatt gttaaaacta attttttcta atatgtgttt
    21901 ggttgttttc attgttataa gttgtccacc tctatttaat aaaatagata cttgcttttc
    21961 ttctggtaaa gcgtttaagg tattagcaaa atcactatat ccatcaacct cagcattatt
    22021 tacctttact atagcatcac ctaatctaaa tggagatgct tctccagggt ttctaactat
    22081 gatatttttt aattctgcat caatttgaag tacatttcca attggtatta aatattcagg
    22141 ttcatttttt tgtaaatttt gtgcataaat taaattattt gagaaaaaat ataataaaaa
    22201 taaaaatgtt aaaacaattg taaattttga cttagctaag ttggtttttt tacttaaagt
    22261 attatcttta aaatgaaaat ttttaagttg cataaaacac ctctttctta ttaaattatt
    22321 ttaataatag aattgaagtt ttcttgctgc ttctatatta tgttgtgcaa gaattatgtt
    22381 ttgatgcttg gaaaactgac tcaataacac cttagttttc ataaaaataa ataaaataaa
    22441 tagtgtatat gataaaataa atacatattc atataaataa aaataaacaa taactgtcta
    22501 gcataagaaa ggagcaaaaa atgaaaaaat atagattgtt agttacttgt ataattgcca
    22561 tgggaatcat aattatagga acatcaaatc cagttagtaa tttttacaag actacaaatt
    22621 tgattagttc aggaaatatc acaaatgaaa aagcagataa aggtgaaaat tcaaacaaaa
    22681 ataataaaga taaagaagaa gacaaatcta actctaaaag taataaagat aagttatcag
    22741 aggacaaatc gtcagataat aaaaatgaaa ttcaaaaaaa taaaaaactt ttaatttgta
    22801 tagacccagg gcatcaagga aaaggagata gcaatttaga accagtagct ccaggttctt
    22861 cctctaaaaa agcgagagta tcttcaggaa cagagggtat tgctacaaaa aaaccagaat
    22921 atgttttaaa cttagaagca tctcttgttt taaagaatat attagaatct aaaggttata
    22981 atgtaataat gactagagag acgcatgatg taaatataag taattcagag agagcaatac
    23041 ttgctaatga taaaaaagca gatatggttg taagaataca tgcagatagt ttaaataatt
    23101 cctcaaaaac tggagcatct atattgattc ctgaaaaaga tggaaaatat accgctccta
    23161 tatatgaaga aagtaataaa tgtgctgaat ttatcaaaca gaatatggaa caatcaggta
    23221 tacaaatcaa tggaatcatt caaagaggtg atttgactgg attcaactgg tcaaaagttc
    23281 cagctgtatt agtagaaatg ggctttatga gtaactataa tgaagaccaa atgatgtcaa
    23341 atccagacta tcaaagaaaa atgatgcagt gtatagctga tggattagat gagtatttta
    23401 aatagagtat gaatagagaa aaaattaggt tggtattatt ttcctaattt ttatctctat
    23461 tttttattgt acataacaca aaagtgaagt ttattttagg ttaaattgtc gtaaatataa
    23521 ctaaaaatat tggtaatgct tgatttaagt ttaaaaagaa tatattatag tatagaatgt
    23581 gtaaaatttt tttgagaggt gttgaaatgc tagtagtaga aaatgtgagt catggatttg
    23641 gtgcgagaac aatattagaa aatgtatcct ttagacttag aaaaggtgaa catattgcac
    23701 tagttggtgc taatggagaa ggaaaatcga gttttttaaa tataataaca aagaagctta
    23761 tgccagatgc tggaaatata aaatggtctt caagagctac agtaggctac ttagaccaac
    23821 atacggtatt aagcaagggg aaaactataa gagaagtgct tagagaagca tttaagcata
    23881 tgtttgactt agagcaagaa atgattgcta tgtatgacaa aatgggagaa gctagtgatg
    23941 atgagatgag taagcttctt gaagaaacag cagaaataca gactatactt gaaaatagtg
    24001 gtttttatat gatagatgca aagatacaag aagttgcaaa tggtcttggt cttggagaaa
    24061 ttggtcttga taaagatgta actgacttaa gtggagggca aagaacaaaa gtacttctta
    24121 ctaaacttct acttgaaaat ccaactattc ttatattgga cgagcctaca aactatttag
    24181 acgaagagca tataacatgg cttactaagt accttcaaga ctatgagaat agctttgtct
    24241 tagtttctca tgatattgaa tttataaaca atacatgtaa tgtaatatat catatggaaa
    24301 atggagagct aaatagatat aaaggaaact atgatgaatt tgtaagactt aatgatataa
    24361 agaaacgtca agaagaacaa gcttatgata aacaagttga agaaagaaaa agactagagg
    24421 attttgtagc tagaaataaa gctagagtag caactagagg aatggctaac agtagacaaa
    24481 agcaacttga taagatggaa atattagaaa gaccaaaaga aaaaataaaa cctacttttg
    24541 cttttaaaga tgctagagca gctagtaaaa taatttttga aacagaaaat ttagttttag
    24601 gatatgatga agcattgaca aaaccactta atttccattt agaaagagga aaaaagattg
    24661 ccttaaaagg aatgaatggt attggaaaat ctactctatt aaaaacttta cttgggataa
    24721 taaaaccatt tgaaggaaat gttaaactag gtgactattt agaagttgga tattttgaac
    24781 aagagagctc aagagaaaat agtaatacac ctatggatga agtttggagt gaatttccag
    24841 ggcttacaaa ctttgaagtt agacaggctc ttgcaaaatg tggacttaca aatgaacata
    24901 taactagcca aatgagagta ttaagtggtg gagaagctgc taaggttaga ctttgcaaag
    24961 taatgctaaa aaatataaat ttcttagtat tagatgagcc tacaaatcac ttagatgttg
    25021 aggcaaaaga cgaattgaag aaggctatta aagagtttaa gggaacagtt cttttggtat
    25081 gtcatgagcc agaattttat tcagaaattg ttgatgatgt atggaatata gaagatttta
    25141 caactaaaat cgtgtagtga tttaactttt actgggggga ttttatgaaa aagtttctaa
    25201 atgtactttt ttcagtttgt attagtattg ttataatagt aggtgttatt aattttactg
    25261 ttggatttaa aaaactttat tattttgaca ttgattattt aaacatatca gagttatctg
    25321 gtttatctaa agatgatata aaattgaact acgattatct aatcgattat aacttaaata
    25381 agaatgtatc agagtttaat ttaccaacat taaaatcttc tccacaaggg aagatacatt
    25441 ttgaagaagt taggaatata tttcaaaata taaataaatt agctaaatta ttgttgatag
    25501 tttctttagt aggaattata ttgagtgtaa aaaataaaa
//
LOCUS       DAFNNB010000039        25517 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0039, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000039 DAFNNB010000000
VERSION     DAFNNB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25517
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0039"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            1..117
                     /locus_tag="L3M60_001432"
                     /pseudo
     CDS             1..117
                     /locus_tag="L3M60_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888701.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            118..873
                     /locus_tag="L3M60_001433"
     CDS             118..873
                     /locus_tag="L3M60_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6226111.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQVLLNKHNLSNLAEHRRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLRKCLRYKRDFLENLLEGVSLDLKKYLDVQVKFLSGGQRQSLSLIM
                     SCLTSPSVLLLDEHTAALDPKTSNEVIELTNKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKGKEKEQLTVEEILEKFEYAV"
     gene            1004..2026
                     /locus_tag="L3M60_001434"
     CDS             1004..2026
                     /locus_tag="L3M60_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226112.1"
                     /translation="MINKKRLASLILAGALSVSMLTGCSQGGDSGNSKQESNSKSKEV
                     KKIGITQLVEHPALDATKAGFVKALEKNGFKDGENIDIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIFAISTPSAQAAFNATKDIPILMTAVSDPVAAGLVKTLEKPGTNVSGTSDFV
                     SVDKGLELLKIFAPKAKTIGVMYNTSEVNSKVQVDALKEYASKNGFKVVEKGITTSNE
                     VNQGISSLVGKIDVLYVPTDNLVASSMPIVSKIATENKIPVIAAESGPVEKGALACQG
                     INYEKLGYKTGEMAVKILNGESVSDMPVATSDDTDIIVNEDILKALGMEKPSNKNISY
                     VKTKQE"
     gene            complement(2188..2745)
                     /locus_tag="L3M60_001435"
     CDS             complement(2188..2745)
                     /locus_tag="L3M60_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBN6226113.1"
                     /translation="MTEKEKMLSGKGYYANDELLVKEREYCKKLTRLFNNTLEGEYGK
                     REAILRQLFGSVGKQINVEQNIRCDYGYNIHVGENFFANYDCIFLDVCKIEIGDNVML
                     APSVQIYTAYHPIDAQLRNSGIEYGSPVKIGDNVWIGGGVIITPGVTIGDNVVIGAGS
                     VVTKDIPPNTVAVGNPCRVIKKIEE"
     gene            complement(2772..3827)
                     /locus_tag="L3M60_001436"
     CDS             complement(2772..3827)
                     /locus_tag="L3M60_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBN6226114.1"
                     /translation="MSLYVDIEKDLSSFKLKVEIKQEKGTLGFLGESGSGKSMTLKCI
                     AGLEKPTRGKIVLNDRVLFDSEKKINLSTQDRKVGFLFQNYALFPHMTVSQNIELGLL
                     KLSKSEKKEIVARYLDILKLNGFEGRYPWQLSGGQQQRVALARALATSPDILLLDEPF
                     SALDHHLRSNMEKELMNMLKDYKGDILFVTHDIEEAYRVCDDIIVYNKGEGLPKRPKK
                     ELFESPKYLIEAKITGCKNISQLNRIDKNTIYAIDWGCELTLDREIVDNIEYVGIREH
                     HIKVLDSNEDLNEKLCFELINIIENPFTYTMYVRKTNLSNECVPIQIELEKSKMRFKK
                     GDSIYLDFPQEYLFCFK"
     gene            complement(3828..4511)
                     /gene="modB"
                     /locus_tag="L3M60_001437"
     CDS             complement(3828..4511)
                     /gene="modB"
                     /locus_tag="L3M60_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBN6226115.1"
                     /translation="MGTDWSPLWISLKTALLSTVITFVIGIFVSYIMANYRGKWKGLI
                     DGLFTLPLILPPTVVGFFLLLLCGKNGFIGKFLLSFDKTLIFSWTATVISAVVVSFPM
                     MYRTSRSAFEQIDNNMLSAARTLGLNEWKVFFKVAVPLAWPGIIGGLVLSFARALGEF
                     GATLMIAGNIPGKTQTMPIAIFFAVEGGDMNKAMMWVMIIVVISIAMILLLNYWSEFQ
                     QKIIGKRCG"
     gene            complement(4660..5472)
                     /gene="modA"
                     /locus_tag="L3M60_001438"
     CDS             complement(4660..5472)
                     /gene="modA"
                     /locus_tag="L3M60_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6226116.1"
                     /translation="MKKILGILGLVACLTLGTVGCNSNESKKDDGKQEKTNKSSDSIE
                     LNVSAAASLKEAMAKIEEEYKKIDSNVKLTVNYGASGSLQQQIEQGAPCDLFISAGQK
                     QMKALDEEKLLVSDTMKDLVKNDLVLISSADSNVTGMKDLTTDKVKKIAVGEAESVPA
                     GKYADEVLTNLNLKDKLKDKLVFAKDVKEVLAWVQSGNADVGFVYFSDTVNNDKIKVV
                     EKTDEKTHSPITYPVSVIKASKNVDAAKKFEEFLLSEEGQKIFEEFGYKKVE"
     gene            5665..6594
                     /locus_tag="L3M60_001439"
     CDS             5665..6594
                     /locus_tag="L3M60_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226117.1"
                     /translation="MDSLSSLTPVEVAELLKITKNTVYELIKRGELPSYKVGKKIRVD
                     IKDVEEYINIQKTGKVKDTHFNNKNIDINNNFKIIISGQDVILDILARSVEKKLDGIS
                     TFRSYIGSYNALYELYNGRVSIASSHLWDFETDEYNSTFVKKLLPGIPCVLINLAYRM
                     QGFYVAKGNPKEIKTWDDLTKSNITIVNREKGSGTRVLLDGKLRLLNFNGKHIKGYDN
                     EELSHLGVASTVSRGIADVGLGNEKAALQVNNIDFIPLHKERYDLVIKKEDLQNPVYQ
                     TIINIINSPEFKAELQGIGGYDLTDTGKTIAKV"
     gene            complement(6756..7205)
                     /locus_tag="L3M60_001440"
     CDS             complement(6756..7205)
                     /locus_tag="L3M60_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226118.1"
                     /translation="MKNKNNKDKNMFNIKVLRVLGLLLILFDYTGDTLNFYNFFAKPL
                     VSGNLYKTTGADCIVFLLFIVECVYLVGRYILKVKINWSDIIESEKDNFINNLLVEKI
                     QEESNIVYNLLNSYVLPYIYKKGKGLMSKTYHMLIYIKEKYYYVNIY"
     gene            complement(7407..8387)
                     /locus_tag="L3M60_001441"
     CDS             complement(7407..8387)
                     /locus_tag="L3M60_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6226119.1"
                     /translation="MKNSFKNYISLRNKFIIISIVFLLILLILSYIFGKLVYNSSVGL
                     EQLIKNEDIVKIFSERKDKPLDKLKNYKTAELMIQSSNNYKLESLFITSNIKTRDTII
                     LVHGIGSSYYEMLKVAYGYLDKGYNVLVYNQRNTGNSGGDNYTFGLYERYDLDSLVKF
                     VKNKFPEGRLGVHGFSMGAGTAAMHTEINSKNEKVDFYILDSPYSEMKDAIRMGVLEK
                     RIPGILIDYVVTCGDLYNKFKSGFWYSDVKPYESVEKSNVPILFIHGTKDTVCNYQNS
                     KKMYDLVKHDKKELWFIEGIGHVDGFEHDSTVYFNKIFKFIDSNVLSDKS"
     gene            complement(8599..9459)
                     /locus_tag="L3M60_001442"
     CDS             complement(8599..9459)
                     /locus_tag="L3M60_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HBN6226120.1"
                     /translation="MAMKWRDYADDVNLFEDFDKTIKPLTDEQRRKNINTLIAYFSKE
                     VPSKIYNLSNDEIKPRDILRGLLNVYPPKEIAPEILNMLHNLLLIECEERELVDVNDI
                     EEVEEGIAIWRGNITNLRADAIVNAANNKLLGCLQPLHLCVDNEIHSCAGPRLREDCN
                     KIIKKQGHLEYTGDAKITRGYCLPAKFVVHTVGPIVSGGQPSKEQEKQLLHCYKSCLN
                     TIKEIDEIKNIVFCGISTGVFGYPKKEAANLAVSRVRLWLKENPEKNLKVVFNVFTEE
                     EEEKYRRIFK"
     gene            complement(9877..11205)
                     /locus_tag="L3M60_001443"
     CDS             complement(9877..11205)
                     /locus_tag="L3M60_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBN6226121.1"
                     /translation="MEKFSVVIAGGGSTYTPEIILMLLDNLDRLPLRAIKLYDNDEER
                     QNKVAKACEILIKEKNPNIEYLATTCPKEAYTDVDFCLAHIRVGKLEMRELDEKIPLK
                     HGVVGQETCGPGGIAYGMRSIEGVLENIDYMEKYSPNCWMLNYSNPASIIAEAVRRLR
                     PNSRVINICDMPIGMEHNIARIAGLESRKHMDIRYFGLNHFGWYTSIKDKKGNELLPK
                     LVEHIKEYGFINGEEGMKNDKKDSWFETNLFTKEIVKTDLTTIPSSYLKYYLFPDYVV
                     NHSDVNYTRANEVIDGREKEVFGSCALIEKQGHSKGSGLKIGIHAEFIVDLATALAFN
                     TKERMLLIVQNNGAIENIQDDAMVEIPCIVGKDGYEPLSIGEIPTFQKGLIEQQLAVE
                     KLVVDAWIEKSYNKLWQALMLSKTVPSATVAKELLDDLIEANKNYWPELN"
     gene            complement(11353..11682)
                     /locus_tag="L3M60_001444"
     CDS             complement(11353..11682)
                     /locus_tag="L3M60_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HBN6226122.1"
                     /translation="MVPEIEEKVFSIISEAGDAKSDVMMSLKEIKKGDYNKAKHLLNS
                     ASEKIQTASKHHLELLSNAMNSEDSGTDFLVVHSEDHYSNALFAHSLVSELVDIFEMM
                     DPRINKN"
     gene            complement(11706..12995)
                     /locus_tag="L3M60_001445"
     CDS             complement(11706..12995)
                     /locus_tag="L3M60_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6226123.1"
                     /translation="MEALQNNLRKFLLPIAQKIEKQRHLQAIKEGMISITPIIIVGSL
                     SMLFMALNNMLPEGSAKTLLSENMDTLLIPNKFTMSLLSIYSAFFIAQALAKKYNLNH
                     VEIGMTAVIAQLVVCGQVIDGALDTSYLDAQGLFVSILVALLVVDITKFMNDKNLVIR
                     FPKEVPSVVNKSFRNLTPMIVCIMLFTAIAAITKNVLGQPLPAIIMNFLAPAISSVDN
                     VFAVTIILFITQLLWFFGLHGAAITSSIWMPIAATYMAENATLIAAGGDPKYVFTIGF
                     YYGFLQVTGSGITLGLVYLMSRSKCKSLNSMGKVVILPSLFGINEPVIFGTPIVMNPY
                     MFVPFVFGPVLVGALNFMALKVGLVGLPIAEPPGFLPPGVAAFLMTLDWKAIVLVFAS
                     IILMTLIYYPFFKIMEKEELNKNAELATALDDDSFDF"
     gene            complement(13011..13337)
                     /locus_tag="L3M60_001446"
     CDS             complement(13011..13337)
                     /locus_tag="L3M60_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6226124.1"
                     /translation="MNILLVCANGASTGVLVEKMKNFCSEHEKLKTKTINIEATSFEN
                     LKSYIQANDTDVVLVAPQIRFKEDDVVEACKNYKIAVGLIDTKHYGRMDAPSVMKSAI
                     ELYKNR"
     gene            complement(13455..14084)
                     /locus_tag="L3M60_001447"
     CDS             complement(13455..14084)
                     /locus_tag="L3M60_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226125.1"
                     /translation="MQDKILRDKKANDPWARMTTRNGFSADEIISSFQKAIRRNMVEE
                     ACEFAYELYISSPELEDKLWRRILTISVEDIGMGDPSAIIIINNLNQVRKEYSYADGD
                     RPLFFIHAIRYLCACEKDRSSDLLKNIIIKGFAMGKVPEIMDVALDKHTYRGLEMGRD
                     SFHFLNEASIVIPEKEVDNDYKERYLKILEQYNSEEVIDTAFRFNHWQY"
     gene            complement(14298..16955)
                     /locus_tag="L3M60_001448"
     CDS             complement(14298..16955)
                     /locus_tag="L3M60_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6226126.1"
                     /translation="MIYKKDKVMKVLESSYRENEKGITANQLSEKTGIARNNISTYLN
                     QLFNEGMVTKIKGRPVYYIPANLNLNKNELLDTQNELLGIKKGTTVENKIKKDSFETL
                     IGKEHSLRPIIEKCKVAVLYPTNGLHTIIYGETGVGKSMIARYMYDYSIDSGIRKNKA
                     PFVTFNCADYANNAQLLMGHIFGVEQGAYTGAESSRKGLLEIANGGILFLDEIHRLPA
                     EGQEMLFTFIDKGTFKRLGDATKERESKVLIICATTENPTSTLLDTFNRRIPMKIEIP
                     SLRDRTMIERIELIKNFFKEESKSISISIKVHKEIIKSLLLYDCKNNVGQLQNDIKLA
                     VANGYLKYKRDNKEPVYIEKKYFDKSINEVSDDYREKSIKADEVITHRIEYFVFTSLG
                     EEEVINYNDISIINAVNTNINKSVEMNITDASLFNNVKFKNMCESIRQIIKEDEEIDI
                     SESIFKSMAIYIKSVLDKSSSTNNIDLNQIRRNNRSEFKTALKIVGIIENEFDLFLSI
                     ETVAYITLFIVQAKQDEQNKIVNNINIIVAMHGETTASSMVKAVGDIIGNCNAISFDL
                     KLDKSYDEVIVDFKNLINNIDNKDVLLFTDMGSLNSFDEIIKKEKKCGVMVIPMVTTL
                     TVLEAVQKANMGLPLNDVYNSITNTRKYYFGTNEIQNKENLSKTIIIASHVSEGVDNK
                     TRKILEEKMNRYLDGIDIISVPYKTEKDLSLNITKLKESSNIVALINEQRINIRGIDY
                     ISKKDIDKDKSINKLKNIIKISIGYDDVVEGLKTSLKSSNYNRIFKDIKYVSDELFLV
                     FNIEKKYDKVIGLMMHLAFMVDGLIGNTREIEKLDKEKMLNYHKSLSKIKDIVSQLDK
                     KYNIEINEKECYQILLILEYAEIIEKDYQ"
     gene            complement(17315..18259)
                     /locus_tag="L3M60_001449"
     CDS             complement(17315..18259)
                     /locus_tag="L3M60_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226127.1"
                     /translation="MNREKMLEIKNLKQYFHLDKNTTVKAVDDISFDIYKGEIFGLVG
                     ESGSGKSTTGKTIVRLHESTGGEVIYKGNCISDKKTYKFIKKDVNKSMQIIFQDSTSS
                     LNPRMTIADIISEPLKIQGIYKNKTDRMNKVYEMLNLVGLDRSYANKYPSDFSGGQRQ
                     RIGIARALSVDPEFIIADEPIASLDVSIQAQIVNLFKKLQQEKNLTCLFIAHDLSMVR
                     HISDRIGVMYNGKLVELADSNELYNNPIHPYTKSLLSAIPVPDPRYAKSRTRIEYNSN
                     VYDCSNEKSLSWIEVSDGHFVYSSKADINKYQQNLKVV"
     gene            complement(18252..19277)
                     /locus_tag="L3M60_001450"
     CDS             complement(18252..19277)
                     /locus_tag="L3M60_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226128.1"
                     /translation="MEHLLEVNNLSVNFKVEEGEVQAVRNVSFNLKKGETLAIVGESG
                     CGKSVLCKSLMRILPYNGYIKNGEVLLKSSNLVKKSEKEMEDIRGKNISMIFQDPMTS
                     LNPTISIGKQIAEAVIIHQGISKSEAKKRAIELIELVGIDNPEKRFKQFPHHFSGGMR
                     QRIVIAIALACNPDVLIADEPTTALDVTIQAQIIDLIKDLQHKIGLSIIFITHDLGVV
                     ATIADRIAVMYAGKIVEIGTVEDIFYDPRHPYTWGLLGSLPTLDSQDDYLYNIPGMPP
                     NLLHPPKGDAFAIRNKNALKIDYEKEPPMFKINDTHSAATWLLHPDAPKIDVPVRVNC
                     GRVISNE"
     gene            complement(19352..20920)
                     /locus_tag="L3M60_001451"
     CDS             complement(19352..20920)
                     /locus_tag="L3M60_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226129.1"
                     /translation="MKLKKLKVLSLVMILSLMAGCSSGGDKDKKADKPKDGKVLVYGS
                     NDYTSINPALYEHGEINSLIFNGLTAHDENNKVVPCLAKDWKFDEATNTYTFNLRDDV
                     KWHDGEKFTANDVKFTIETIMNPDNASEIASNYEDITKIDVVNDNTIKITLKAPNTAM
                     LDYLTVGILPKHALEGKDIATDEFNQKPIGTGPFKLEKWDKGQSITLVKNSDYFVKEP
                     GLDKVVFKIVPDDKAKAMQLKSGELDLAQVTPKDMSNFEKDEKNFKVNIMKTADYRGI
                     LYNFNSKFFKDKKAKGLPNALSYAIDRKAIVDSVLLGHGVPAYSPLQMGPYNNPDIEK
                     FEYNPEKAKQEIEKLGWKLGSDGIYEKEGTKLAFEITAGESDQVRVDMAKICAQQLKE
                     IGVDAKAVVVTETDWANQDAHLIGWGSPFDPDDHTYKVFGTDKGANYSAYSNHTIDKI
                     LQKARETENKDEKLKLYKQFQVEMTKDMPYTFIAYIDAIYVGKPNIKGLTPDTVLGHH
                     GVGIFWNIADWTIE"
     gene            complement(21033..21944)
                     /locus_tag="L3M60_001452"
     CDS             complement(21033..21944)
                     /locus_tag="L3M60_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226130.1"
                     /translation="MKPQYSDFEIVGENYILVAKDEHVETKKNLFRKIKELPYISIII
                     LSIIVIGSVFSSFIMTHEPTYMDLASSNLAPNKTFLFGTDSMGRDIYSMIWYGGKISL
                     FIGLFSTVISTVIGIVYGSISGSASEFVDDAMMRFTEIILSIPSILIIIFVQAILGNS
                     NPVSMSIVIGITSWMNISKIVRTEVRQIRNSEYILAAKSMGGGFFYVLKQHLLPNFVA
                     SIMFMVVTNIGAAIGTESTLSFLGIGLPIEIVSWGSMLSLSEEALLSNRWWIILIPGI
                     FLVTTLVCITNIGNYIRKNNNKKSSNL"
     gene            complement(21928..22914)
                     /locus_tag="L3M60_001453"
     CDS             complement(21928..22914)
                     /locus_tag="L3M60_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226131.1"
                     /translation="MLKDILKKLLQFILVMFLLSFIVFYMARLAPGDPLISYYGDGVE
                     RMSTQEKENAMKKLGLDEPIYNQYIKWISNASKGEFGISFKYKQNVTSVINDVYINTI
                     ILGGSGYILTFVLALLLGIFCTLHEDKLIDRVICKLGTITNCIPSFWVALVLILVFSI
                     NLSILPSSGAYSMGEESSILSRISHLILPLTVLVLSHLWYYTYMIRNKLLEEIREDYV
                     LLCKAKGLNNRTIVFKHCLRNIMPSYISIMAISIPHILGGTYVVEKVFSYPGLGTLCF
                     ESAKYHDYNMLLVLCLITGALVVFGNMLAQIINNKIDPRMKYDRGDTNEAAI"
     gene            23275..23676
                     /locus_tag="L3M60_001454"
     CDS             23275..23676
                     /locus_tag="L3M60_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN6226132.1"
                     /translation="MLLKELYSKTMYTVEENFKENGLTHQQIIVIKLIAHNQELTISQ
                     LCDEMSLAKGTVSGIISRLEQIGYIEKFKKSNDKRNTYVKFTTTGFEFATNFKIKMQE
                     SFDDIFKNCDEKELSDLVKNLRTILAKVKER"
     gene            23679..24077
                     /locus_tag="L3M60_001455"
     CDS             23679..24077
                     /locus_tag="L3M60_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03987 family protein"
                     /protein_id="HBN6226133.1"
                     /translation="MNYKLILAIVFITSALIFYTIGVFGERKAKILKKKHVIIFWLGF
                     IFDTLGTFTMSNIANSHTFEVKSALSQNLHSITGLLAIVLMLFHASWATFVLYKNDEE
                     KKKFFHKFSIVVWTIWLIPYFIGMFIGMAG"
     gene            complement(24400..24624)
                     /locus_tag="L3M60_001456"
     CDS             complement(24400..24624)
                     /locus_tag="L3M60_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HBN6226134.1"
                     /translation="MREKVEKVLEEKIKPVLQRDGGDVELIDVNENGVVLVRLQGACS
                     GCPGATMTIKAIIENVLVSEVPGVTQVLGV"
     gene            complement(24659..>25517)
                     /locus_tag="L3M60_001457"
     CDS             complement(24659..>25517)
                     /locus_tag="L3M60_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HBN6226135.1"
                     /translation="NAPWRGGSLANLSVKFKFKGITAHAAQAPHNGRSALDAVEIMNV
                     GANYLREHVIDSIRMHYVITNGGGRPNVVPGFAESWYFIRGKKAKDAEHVLDRLIKVA
                     QGAAMMTETEMEYKVTDGIYDYIPNQCLTDLVYNNMVFVGCPKTTPEEEEFAKKLCDT
                     LTREERLGVATSFQNDKSIVESYIHKGIVDGDKDKGLAGSTDVGDVSYVIPVAQFAMA
                     AWPVGIASHTWQSCSSAGSNIGFSAMINSAKVLACSAYDVFMDTKIIDEAKIEFDKSL
                     DGQKFKPLV"
BASE COUNT         8346 a         4372 c         2897 g         9902 t
ORIGIN      
        1 taataactgc catagttata atagctttct tagctactgg taacttgaat atttcactaa
       61 aaaaaaacaa atgcaaagct agtaccaaaa ataaatcaga aaaaagaggt gttgtagatg
      121 ttacaaatta aaaatctttc aaagagtttc cccaatccat acggtgaacc taatactata
      181 tttgaaaatt tatcaattga tatagaagat ggtgaatttg taagtatcat aggtagtaat
      241 ggaactggta aatctacttt attaaatata atctctgggc ttataaagga atcttctggt
      301 caagtattat taaataaaca taacttgtct aatttagctg aacacagaag aactcaaata
      361 gtaagtagag tttttcaaga cccaagtctt ggaacttgtc cttctatgac tgttagggaa
      421 aacttgtctt tagctcttaa taaaggaaaa ctattaaacc taagaaaatg tcttcgttac
      481 aaaagagatt ttttagaaaa tttattggaa ggtgtatctc ttgatttaaa aaaatacctt
      541 gatgttcaag taaaattttt atcaggaggt caaagacaat cattatcact aattatgtcg
      601 tgcttaacaa gtccatcagt tttattactt gatgaacata cagcagcact tgacccaaaa
      661 acatcaaatg aagtaattga attgactaat aagattgtaa gggaaaaaaa tataactaca
      721 cttatggtta ctcacaactt aaaacacgct cttcagtatg gtgatagatt gataatgctt
      781 cataagggtg aagttgtatt agatgtaaaa ggtaaagaaa aagaacaatt aacagttgaa
      841 gaaatcttag aaaaatttga atatgcagtt taaaataata tatctattgt attaaactca
      901 gctttaaaat aaagttaaaa ctataataat tatggttact ttacaataat tcatatgaat
      961 atatgaacat atataataat aaatttttga gggggatata aaaatgatta ataaaaaaag
     1021 attagcaagt ttgattttag ctggtgctct tagtgtatct atgcttacag gttgttcaca
     1081 aggaggagac tctggtaatt caaaacaaga aagtaattct aaaagtaaag aagttaaaaa
     1141 aattggtata actcaactgg ttgaacatcc agcattagat gcaactaagg caggttttgt
     1201 aaaagcatta gaaaagaacg gatttaaaga tggagaaaat attgatatag acttccaaaa
     1261 tgctcaaaat gatatgccta ctacacaaag tattgcaagt aaatttgcat ctgacaagaa
     1321 agatttaata tttgctatat ctactccatc agctcaggca gcatttaatg ctactaaaga
     1381 tatacctata cttatgactg ctgtcagtga ccctgttgct gctggattag ttaaaactct
     1441 tgaaaagcca ggcacaaatg tatctggaac atcagatttt gtttctgtag ataaaggttt
     1501 agaacttctt aagatatttg ctcctaaggc aaaaactata ggagttatgt acaatactag
     1561 tgaagtaaat tccaaagttc aagttgatgc tttaaaagaa tatgcatcta aaaatggctt
     1621 taaagttgtt gaaaaaggta taactacttc aaatgaagtt aatcaaggta tttctagttt
     1681 agttggtaaa atagatgtat tatatgttcc tacagacaac ttagtggctt cttctatgcc
     1741 tatagtttct aaaatagcta ctgagaataa aatacctgtt atagcagctg aatctggtcc
     1801 agttgaaaaa ggtgctcttg cttgtcaagg tatcaactac gaaaaactag gttacaaaac
     1861 aggtgaaatg gcggttaaaa ttttaaatgg cgaatcagta tctgacatgc ctgtggctac
     1921 atcagatgat acagatatca ttgttaatga agatatatta aaagctttag gtatggaaaa
     1981 accttctaat aaaaatattt cttacgtaaa aactaaacaa gaatagaaag atattttcta
     2041 tatacgatta atatgtccgt ttctataaat taatttatag aaacaggcat atatttttgt
     2101 tttaatgtat tatttattta aagattatat aaacgctctt aatttatttt tataaatttt
     2161 acttgtataa actttgtaca atcatattca ttcttctatt ttctttatta ctctacatgg
     2221 atttccaaca gctacagtat ttggtggtat atcttttgta actacacttc ctgcacctat
     2281 tactacatta tcacctatag taactcctgg tgttatgatt acgcccccac ctatccatac
     2341 attatcacct atttttacag gagagccata ttctatacct gaatttctaa gttgtgcatc
     2401 aatcggatga taggctgtat agatttgaac gctcggagct aacattacat tatcacctat
     2461 ttcaatctta caaacatcca agaaaataca gtcataattt gcaaagaaat tttcacctac
     2521 atgtatgtta tatccataat cacacctaat gttttgttct acatttattt gctttccaac
     2581 tgaaccaaat agttgtctta aaatagcttc tctttttcca tattcaccct ctaaagtatt
     2641 attaaatagt ctagttaatt ttttacaata ctctctttct tttactagca attcatcatt
     2701 tgcatagtaa cctttcccag acaacatttt ttctttttcg gtcatataat ttactccttt
     2761 ttattgtaat tttacttaaa gcaaaataaa tattcctgtg gaaaatctaa ataaatgctg
     2821 tcacccttct taaatctcat tttacttttt tctaattcta tttgaatagg tacacattca
     2881 ttagataaat tagtttttct aacatacata gtataagtaa atggattttc tataatatta
     2941 atcaattcaa aacataactt ttcatttaaa tcttcattag aatccaagac cttaatgtga
     3001 tgctctctaa ttcccacata ttctatatta tcaactattt ctctgtctaa tgtcaactcg
     3061 catccccaat ctatagcata aatagtattt ttatctattc tatttaattg ggatatattt
     3121 ttgcaccctg ttatcttagc ttctataagg tactttggag attcaaagag ttctttttta
     3181 ggtctctttg gaagaccttc tcccttgtta tacactataa tgtcatcgca aactcggtag
     3241 gcttcttcaa tatcatgagt aacaaatagt atatctccct tataatcttt taacatattc
     3301 ataagttctt tttccatatt gcttcttaag tgatggtcca gtgctgagaa aggttcatct
     3361 aaaagtaata tatctggaga tgttgccaat gctctagcta gtgctaccct ttgttgttga
     3421 ccaccagata attgccaagg gtatctaccc tcaaacccat ttaatttaag tatatctaaa
     3481 taccttgcta ctatttcctt tttttcactc ttgcttagtt ttaaaagccc aagttctata
     3541 ttttgactta cagtcatatg tggaaacaaa gcatagtttt gaaataagaa acctaccttt
     3601 ctatcttgtg ttgataaatt tatcttcttt tcagaatcaa atagaactct atcatttaat
     3661 acaattttac ctcttgttgg cttctcaaga cctgctatac attttaatgt catacttttt
     3721 ccagaaccag attctcccaa aaaccccagc gtaccttttt cttgtttaat ttctaccttt
     3781 aatttaaaag aggataaatc tttttcaata tctacgtata agctcaacta tccacacctc
     3841 tttcctataa ttttctgctg aaattccgac caataattta gtaataaaat cattgctata
     3901 gatattacta caataatcat aacccacatc atagctttat tcatatctcc accttcaaca
     3961 gcaaaaaata tagctattgg catagtctga gtttttccag gtatatttcc agctatcata
     4021 agcgtagcac caaactcacc aagagctctg gcaaatgaca gcactaatcc accaattatt
     4081 ccaggccatg ctaatggaac tgcaacctta aaaaatactt tccattcatt aagacctaat
     4141 gtcctagcag ctgacaacat attattatca atctgttcaa aagctgacct tgaagttcta
     4201 tacatcatag gaaaagatac cactactgct gaaattacag ttgctgtcca actaaatatt
     4261 aaagtcttgt caaaacttaa taaaaacttc cctataaatc cattcttccc acataacaat
     4321 aataaaaaga atcctacaac tgttggtggc aaaattagtg gaagtgtaaa tagtccatct
     4381 ataagaccct tccacttacc tctatagtta gccataatat atgacacaaa tattcctatt
     4441 acaaaagtta ttactgttga caaaagagct gtcttaagtg atatccataa aggtgaccaa
     4501 tctgttccca tctttccctc cttaatttta aatggaaata gtgaaattaa taactctatt
     4561 aatttcacta tttccatttt attataatac acaattattt tcaattttca tttatttata
     4621 tataatttac actcaattat acataattta tttataagtt tattctactt ttttatatcc
     4681 aaattcttca aaaatctttt gcccttcctc acttagtaaa aattcttcaa atttcttagc
     4741 agcatctaca tttttactag ctttaattac cgaaactgga tatgtaatag gtgaatgagt
     4801 tttttcgtct gttttttcaa ctacttttat cttatcatta ttgacagtat cactgaagta
     4861 aacgaatcca acatcagcat ttcctgattg tacccatgct aaaacttctt ttacatcttt
     4921 tgcaaatact agcttatctt ttaacttatc ttttaaattt aaattagtta acacctcatc
     4981 tgcatactta ccagctggaa cactttcagc ttcaccaaca gcaattttct taactttatc
     5041 tgtagtcaaa tctttcatac cagttacatt actgtcagca gaagaaataa gaactaaatc
     5101 attttttact aaatctttca tagtatcaga aactaataac ttctcttcat ccaaagcttt
     5161 catttgtttt tgacctgctg aaataaataa atcacaaggt gctccttgct ctatttgttg
     5221 ttgtaatgag cctgatgctc catagttaac tgtaagcttt acatttgaat caattttttt
     5281 atattcttct tcaatctttg ccattgcttc ttttaaactt gcagccgccg atacatttaa
     5341 ctctattgaa tcactacttt tatttgtttt ttcttgtttt ccatcatcct ttttgctttc
     5401 attactattg catccaactg tccccaaagt taaacatgca actagtccta atattcccaa
     5461 tatttttttc ataccaatct ccctttttgt aaatttaata catattttaa catgttttat
     5521 tatgttttat catgttttgt catgtatagt ttaattatat atacctgata ataaattttc
     5581 aagtaattta tttacattac ttttaaatat tttgctacat tttccttttt ttaatgttaa
     5641 aataacctta tatggaggtg gattatggac tcattatcat ctttgacacc tgtcgaggta
     5701 gctgaattat taaaaataac aaaaaatact gtctatgaat taatcaaaag aggtgaactt
     5761 ccttcttata aagtagggaa aaaaataaga gttgatatta aagatgttga agaatatatc
     5821 aacatacaaa aaactggtaa agtaaaagat acacatttta acaataaaaa tatagatata
     5881 aataataatt ttaaaattat tatctctggt caagatgtaa tattagacat tttagctagg
     5941 tctgttgaaa aaaaattgga tggaataagt acatttcgct cttatatagg tagttataat
     6001 gctttgtatg agttatataa tggcagagtt tccatagcat catcccatct atgggatttc
     6061 gaaacggatg aatataatag cacttttgtt aaaaaactat tgccagggat accatgtgta
     6121 cttataaatc tagcttatcg aatgcaaggt ttctatgttg ctaaaggaaa tcctaaagaa
     6181 ataaaaacct gggatgattt aacaaaatca aacataacca ttgtaaatag agaaaaaggt
     6241 tctggaacta gggttctact agatggtaag cttaggcttc taaactttaa tggtaaacat
     6301 ataaaaggtt atgataatga agagttatca catcttggag ttgcaagtac tgtttctcgt
     6361 ggaatagctg atgttggttt gggaaatgaa aaagctgctt tacaagtaaa taatatcgac
     6421 tttattcctc ttcacaagga gcgttatgat ttagttatta aaaaagagga cttacaaaat
     6481 ccagtttacc aaactataat taacataata aactctcctg agtttaaagc tgaacttcaa
     6541 ggcattggtg gatatgattt aaccgatact ggaaaaacta tagcaaaagt ttaaaattaa
     6601 aatcgcaatc tattttataa gatttataat ttttcacaaa atttataact taaacacgaa
     6661 ataaattgcg atttttagta tctcaaaaaa tttatatctt tcattttatc atctaatata
     6721 aaatttaata taattaattg tgtaagtata ttatattaat atatatttac ataatagtat
     6781 ttttccttaa tatagattaa catatgatat gttttagaca tcaatccctt tcctttctta
     6841 tatatatatg gtaacacata tgagtttaat agattatata caatattgct ttcttcttgt
     6901 attttttcaa ctaacaagtt atttataaaa ttatcttttt cgctctcaat aatatcactc
     6961 caatttatct taacttttaa tatatatctt cccactaggt atacacactc tacaataaat
     7021 aataaaaata caatacagtc agcacctgtt gtcttatata aatttcccga tactaatggc
     7081 ttagcgaaga aattataaaa attcaaagta tctcctgtat aatcaaaaag tattagtagt
     7141 agtcctagga ctctcaatac cttaatatta aacatatttt tatctttatt atttttattt
     7201 ttcatatata ctacctctct tttctttaga ttttattgta atctaatact aactctttta
     7261 tttgaggtat aaatgaattt aattggtaat tattttcatt tttactagaa attaattcac
     7321 attaataaag aaaacttata tttaaacata aaaatagcct atctattttt aataaataca
     7381 aaaataaata agctactaat attcaactaa cttttatcac ttaaaacatt actatctata
     7441 aatttaaata ttttattgaa gtatactgta ctatcatgct caaatccatc aacatgacca
     7501 ataccttcta taaaccatag ttctttttta tcatgtttaa ctaaatcata cattttttta
     7561 ctattttgat aattacaaac agtatctttt gtcccatgta taaacagtat aggtacatta
     7621 gatttctcga ctgactcata tggctttaca tcactgtacc aaaatccaga tttaaactta
     7681 ttatataaat ctccacaagt tactacataa tcgattaata tacctggtat tcgtttttct
     7741 agtactccca ttctaattgc atctttcatt tcagaatacg gagaatccaa tatataaaaa
     7801 tcgaccttct cattttttga atttatctct gtatgcatag cagctgttcc tgcccccatg
     7861 gaaaatccat gcactcctaa tcttccttca ggaaatttat tttttacaaa tttaactaga
     7921 ctatctaaat catacctttc ataaagacca aatgtgtaat tatctccgcc actattacct
     7981 gtatttcttt gattataaac taatacatta taacctttat ctaaatatcc atatgctacc
     8041 tttaacattt catagtaact acttccaatt ccatgaacaa gtataatagt atctcttgtc
     8101 tttatatttg aagttatgaa aagactttca agcttgtaat tattagaact ttgtatcatt
     8161 aattcagctg tcttgtagtt ttttaattta tccagtggtt tatcttttct ctcagaaaat
     8221 atttttacta tatcttcgtt ttttattaat tgctctaagc caactgaact attgtacact
     8281 aatttgccaa atatataaga tagaattaat aggattaata gaaacacaat tgatataatt
     8341 ataaatttat ttcttaaact aatataattt ttaaaactat ttttcatatt tatttaccat
     8401 gcaattttcc tatattatat tttttaagta atagcataat tgattatatt attcttataa
     8461 tttctattat acctacttat ttattaaaaa tacaaattat attttattaa tttttactac
     8521 ttgcaaatta ttatcttata caatgaattt gtattatagg attatataat aagtattttt
     8581 aggatttgtt atataatatt atttaaatat tcttctatat ttctcttctt cctcttcagt
     8641 aaaaacatta aatacaactt ttaaattttt ttcagggttt tcttttagcc ataatctaac
     8701 cctacttaca gcaagattag ctgcctcttt ttttggataa ccaaatacac ctgtagaaat
     8761 tccacaaaaa acaatatttt ttatttcatc aatttctttt attgtattta aacatgattt
     8821 ataacaatga agtaattgct tttcttgctc tttacttggc tgaccaccag acacaatagg
     8881 tccaacagta tgtactacaa attttgcagg taaacaataa cctctagtta tcttagcatc
     8941 cccagtatat tctaaatgtc cttgtttttt tattattttg ttacaatctt ctctaagtct
     9001 aggtcctgca catgaatgta tctcattatc tacacataga tgtaatggtt gtaaacaccc
     9061 taataacttg ttatttgcag cattcactat tgcatctgct cttaaattcg ttatattacc
     9121 tctccatatg gcaatccctt cctcaacttc ttctatatca tttacatcta ctaactctct
     9181 ttcttcacat tcaatcaaca ataaattatg taacatatta agtatctctg gagcaatttc
     9241 ttttggtgga tatacattca aaagtcctct aagtatatct ctaggcttaa tttcatcatt
     9301 tgataaattg tatattttag aaggaacttc ttttgaaaaa tatgcaatta aagtgtttat
     9361 attttttctt ctttgttcgt cagttagagg cttgatagtt ttatcaaaat cctcaaataa
     9421 atttacatca tcagcataat ctctccactt catagccatc tctccattct tgtattttag
     9481 gattaaaaat aattatgtat tatgtacata tttatttatt tctataaaca cctacataaa
     9541 tcctgttaaa agtgtaaatt ttttattata caacttctca aactatttct agtgattgaa
     9601 gtaaactatt gtacataaat ttaatccaac taacaagtaa ctaaatccta ccatcctaac
     9661 tgtaaaatac actatatttg ctacttgtat atcatctgta aatacggata ctacttgttt
     9721 agcaagttta ttaatactat aaataacaaa ttatttacaa gtcttgaaga aataagattg
     9781 tcatagacaa acttataaaa tcataaataa agttcctaca aatacattaa ctatgctatg
     9841 tactaaactg taggaacttt aaaaaatata ttatttttaa ttaagttctg gccaataatt
     9901 tttgtttgct tctattaaat catctagtaa ttcttttgca actgttgcac tcggaactgt
     9961 cttagatagc ataagtgctt gccacaactt attatacgac ttctcaatcc aagcatcaac
    10021 cactaatttt tcaactgcta attgttgctc tattagacct ttttggaatg ttgggatttc
    10081 ccctattgat aatggttcat acccatcttt ccccactata catggaatct caaccatagc
    10141 atcatcttgt atattttcta tagctccatt attttgaact atcaataaca tacgctcttt
    10201 agtattaaaa gctagagctg tagctaaatc cactataaac tctgcatgta ttcctatctt
    10261 aagcccacta cctttagaat gaccttgctt ttctatcaat gcacatgaac caaaaacttc
    10321 tttttctctt ccatcaataa cttcattggc acgagtataa tttacatcag aatgatttac
    10381 aacataatct ggaaataaat agtattttaa gtaactgctt ggtattgtcg ttaaatctgt
    10441 ctttactatt tctttggtaa ataaattagt ttcaaaccaa ctatctttct tgtcattttt
    10501 cattccttct tcaccattta taaaaccata ttcttttata tgctctacta attttggtaa
    10561 aagttcattt ccttttttat ctttaatcga agtataccaa ccaaagtgat ttaatccaaa
    10621 gtaccttata tccatatgtt tacgagattc aagccctgca atccttgcta tattgtgttc
    10681 catacctatt ggcatatcac aaatatttat caccctagaa ttaggtctta accttctaac
    10741 agcttctgct attattgatg ctggattaga ataatttaac atccagcaat ttggagaata
    10801 cttttccata tagtctatgt tttcaagaac accttctata gacctcattc cataagctat
    10861 tccacctggt ccacaagttt cttgaccaac taccccatgt tttagaggta ttttttcatc
    10921 taattctctc atttcaagtt ttccaactct tatatgagct aaacaaaaat ctacatctgt
    10981 ataagcttct ttagggcatg ttgttgctaa atactctata ttaggattct tttcctttat
    11041 tagtatttca caagctttag ctaccttatt ttgtctttct tcatcattat catataattt
    11101 aattgctctt aatggtaacc tatctaagtt atctagcaac attaatatga tttctggggt
    11161 atatgtactt cctcctccag ctattactac tgaaaatttt tccataataa tatctccttt
    11221 tctatttaaa aatttactgt atttataaat aaatttataa atactagtac tttaaagtaa
    11281 aagggagttc atttattaaa tctactaatt taataaacta tagccaaact tcaagcacta
    11341 tttacaagaa atttaattct tattaattct tgggtccatc atctcaaata tatctacaag
    11401 ttcagatact aaagaatgag caaataatgc atttgaataa tggtcttcag aatgcacaac
    11461 taaaaaatca gttccactat cttcactatt catagcattt gatagtaatt ctaaatgatg
    11521 ttttgatgcc gtttgtattt tttctgaagc tgaatttaaa aggtgttttg ctttgttgta
    11581 gtcacctttt tttatttctt taagagacat cattacatca cttttagcat ctcctgcttc
    11641 tgatattata ctaaatactt tttcttctat ttcaggtacc atatccttct ccttctaatg
    11701 acttgttaaa aatcaaagct atcatcatct aatgctgtag ctaattctgc atttttattt
    11761 aattcttcct tctccattat cttaaagaat ggataatata tcaaggtcat aagtattata
    11821 cttgcaaata ctaatacaat tgccttccaa tctaatgtca ttaaaaatgc tgcaacccct
    11881 ggaggtaaaa agcctggtgg ttctgcaata ggtaatccta ctaatcctac ctttaatgcc
    11941 ataaagttaa gtgctccaac caatacaggt ccaaatacaa atggtacaaa catatatggg
    12001 ttcataacta ttggtgttcc aaatattact ggctcattta ttccaaataa tgatggtaaa
    12061 atcacaactt ttcccatact atttaaactc ttacacttgc ttcttgacat aaggtacact
    12121 aaccctaaag ttattcccga accagttact tgcaagaaac catagtaaaa acctattgta
    12181 aatacatatt ttgggtctcc tcctgcggct attagtgttg cattttctgc catatatgta
    12241 gctgctattg gcatccatat acttgaagtt attgctgctc catgaagtcc aaagaaccat
    12301 aataattgag ttataaatag tattatagtt actgcaaaaa cattatctac actgcttata
    12361 gctggtgcta agaaattcat tataatagct ggtaacggtt gacctaagac atttttagtt
    12421 attgctgcta ttgctgtaaa taacattata catactatca ttggtgttaa gtttctaaag
    12481 cttttattta ctacactagg tacttctttt ggaaatctaa ttacgagatt tttatcattc
    12541 ataaatttag taatatcaac tactaaaagt gctactaata tacttacaaa taaaccttgt
    12601 gcatctaaat aacttgtatc caatgcacca tctattactt gtccacatac aacaagttgt
    12661 gctataactg ctgtcatacc tatttctaca tgattaagat tatatttttt agctagtgcc
    12721 tgagctataa aaaatgctga atatatggat aataaactca tagtaaattt attagggata
    12781 agtaatgtat ccatattttc agataataat gtttttgcac ttccttcagg taacatattg
    12841 tttaatgcca taaataacat acttaaagaa ccaacaatta ttattggggt aatacttatc
    12901 ataccttctt ttatagcttg aagatgcctt tgtttttcaa tcttttgtgc tattggaaga
    12961 agaaattttc ttaaattatt ttgaagtgct tccatattta ttccccccaa ttatctattt
    13021 ttatataatt cgattgccga cttcataaca gaaggagcat ccatacgacc ataatgctta
    13081 gtatctatta aaccaactgc tattttgtaa tttttacaag cttcaactac atcatcttcc
    13141 ttgaacctaa tttgtggtgc tacaagtact acatctgtat cattagcttg aatgtatgat
    13201 tttagatttt caaatgatgt tgcctctata tttatagttt tagtttttaa cttttcatgc
    13261 tcactacaaa aatttttcat cttttccaca agcacccctg ttgaagctcc atttgcacat
    13321 accaataaaa tattcataat aattaccctc ctgcaatatc taacctcttt tattttctaa
    13381 ttttttatta attaattatt actctaattc ttattaattt ttattttaat tcttattaat
    13441 atttactcta attcttaata ctgccaatga ttaaatctga aagctgtatc tataacttct
    13501 tctgaattat attgttctaa tatttttaag tatctttctt tataatcatt atctacttct
    13561 ttttctggta ttactatgct agcttcattt aagaagtgga atgaatccct tcccatttct
    13621 aatcctctat atgtatgttt atcaagtgcc acgtccataa tttctggaac tttacccatt
    13681 gcaaatcctt ttataattat attttttaat aagtcacttg atctatcttt ctcacatgca
    13741 caaagatatc ttattgcatg tatgaaaaat agaggtctat ctccatctgc atatgaatat
    13801 tcctttctta cttgatttaa attatttatg attattatag ctgatgggtc ccccattcct
    13861 atatcttcaa ctgatattgt aagtattctt ctccatagct tatcttctaa ttctggtgat
    13921 gatatatata gttcataagc aaattcacaa gcttcttcca ccatatttct tcttatagct
    13981 ttttgaaatg atgatattat ttcgtctgca ctaaatccgt ttcttgtagt cattcttgcc
    14041 catgggtcat ttgccttttt atctcttaat attttatctt gcatattatt tactcctttt
    14101 tatttaaatt ttaattattt atattactaa tttagtaata taagcaagta ttatgccaat
    14161 ttttttagac actttaaaac ggtgttattt cagtcatttt tagcacaaaa acttagatac
    14221 tttgtctaag tatattcata tgtgtctaat attaatatta ttttagacta aaaaaattac
    14281 caaataagtt tttatactca ttggtaatct ttttcgatta tttctgcata ttctaatatt
    14341 aacaatattt gataacactc tttttcatta atttctatat tatatttttt atctaattga
    14401 gatactatat cttttatttt acttaagctc ttgtgataat taagcatttt ttctttatca
    14461 agcttttcaa tttctctagt atttccaatc aatccatcta ccataaaagc taaatgcatc
    14521 ataagtccaa ttactttatc atatttcttt tctatgttaa atactaaaaa caattcgtca
    14581 gacacatatt ttatatcttt aaatatccta ttataattag agctttttaa ggatgtcttt
    14641 agaccttcaa ctacatcatc ataacctata cttatcttta taatattttt taatttattg
    14701 atacttttat ccttatctat atcttttttg ctaatatagt caattcctct aatattaatt
    14761 ctttgttcat tgattaatgc tacaatatta ctactttctt taagttttgt gatatttaaa
    14821 cttaagtctt tttctgtttt atatgggaca gatataatat ctattccatc taaataccta
    14881 ttcatctttt cttctaatat ttttcttgtt ttattatcta caccttctga aacgtgtgat
    14941 gctataataa ttgttttaga cagattttct ttattttgaa tttcattagt tccaaaatag
    15001 tactttctcg tgttagtaat agagttgtat acatcattta aaggtagtcc catattagct
    15061 ttctgaactg cttctaacac agttaatgtt gtaaccatag gaattaccat tactccacac
    15121 tttttttctt tctttataat ttcatcaaag gagtttaatg accccatatc tgtaaatagt
    15181 agtacatcct tgttgtctat attattaatt aaatttttaa aatctacaat aacttcatca
    15241 tagcttttat ctaacttcaa atcaaaagaa atagcattgc aatttccaat aatatcacca
    15301 actgctttga ccatactact tgctgtagtt tctccatgca tagcaactat aatattaatg
    15361 ttattcacta ttttattttg ctcatcttgt ttagcttgta ctatgaataa tgtaatatat
    15421 gctacagttt ctatagataa aaataaatca aattcatttt caattattcc aactattttt
    15481 aatgctgttt taaattcact tctattattt cttctaattt gattcaaatc tatgttgttt
    15541 gtagagctac ttttatccaa tacgcttttt atataaattg ccattgactt aaatattgat
    15601 tcacttatat ctatttcttc atcttcttta ataatttgtc ttattgattc acacatgttt
    15661 ttaaacttaa cattattaaa taaagaagca tctgttatat tcatttctac agacttatta
    15721 atattagtat ttactgcatt aataatactt atatcattgt aattaataac ttcttcttct
    15781 cctaatgacg taaacacaaa atattctatt ctatgtgtta taacttcatc tgctttaata
    15841 gacttctctc tataatcatc actcacttca tttatagatt tatcaaaata ttttttctct
    15901 atataaactg gttctttatt atctctttta tactttaagt atccatttgc aacagctaat
    15961 tttatatcat tttgaagttg acctacatta tttttacaat cataaagtaa tagtgattta
    16021 attatttctt tatgtacttt tattgaaata gaaatgcttt ttgattcttc tttgaaaaaa
    16081 tttttgatta actctattct ttcaatcatt gtcctatctc tcagtgatgg tatttctatt
    16141 ttcattggta ttcttctatt gaatgtatct aatagtgttg atgtcggatt ttctgtggta
    16201 gcacatatta ttaatacctt agactctctt tctttcgttg catctcctag tcttttaaat
    16261 gttcctttat ctataaaagt aaacaacatt tcttgacctt ctgctggtaa tctatgaatt
    16321 tcatctaaaa ataatatacc cccatttgca atttccaaaa gtccttttct ggaagattct
    16381 gcacctgtat aagcaccttg ttctactcca aagatatgtc ccataagtaa ttgtgcatta
    16441 ttagcataat cagcacaatt aaatgttacg aaaggagctt tatttttcct aattccacta
    16501 tctatagaat agtcatacat atatctagca atcatactct ttcctactcc tgtttctcca
    16561 tagataatgg tatgaagtcc atttgtagga taaagcactg caaccttaca tttttctatt
    16621 ataggtctta aactatgttc tttacctatt agtgtttcaa aactatcttt ttttatcttg
    16681 ttttctactg ttgttccttt ttttatgcca agcaactcat tttgtgtatc aagcaattca
    16741 ttcttgttaa ggttaagatt tgcagggata taatacacag gtcttccttt tatctttgta
    16801 accataccct cattgaatag ctggtttaaa tatgtactta tattgtttct tgcaattcct
    16861 gtcttttctg ataattggtt agctgtaatc cctttttcat tttccctata tgatgattct
    16921 aaaactttca ttactttatc ttttttatat atcattacaa gctcccctac attataatat
    16981 tttgttaact aaaatcctta tactcacttt gttttttaat aaactcttat tctttaacta
    17041 agattataat ttatcaaact tgtcttattt taaattatat aaaattattg tagaaaaaat
    17101 aaggttattg ataatttttt ctaaaatact attttttaat tttatggtac tttcttaaca
    17161 atatacttta cttgttttta attttattat atttgaattt attatatttg aaaagtgtaa
    17221 ataaaaaacc ctatcttgga gtagttttaa aatttatcca aaaatagggt tttagtttgt
    17281 atttcaaata aatacatgtt caagtttttt tatactaaac aacttttaaa ttttgttgat
    17341 atttatttat gtcagcttta gaagaatata caaaatgtcc atctgaaacc tcaatccaac
    17401 ttagtgactt ttcatttgag caatcataca cattagaatt atactctatt ctagttcttg
    17461 acttagcata tctagggtca ggaacaggaa ttgcagaaag tagtgatttt gtatatggat
    17521 gaatgggatt attatacaat tcatttgaat ctgccagttc tactaatttt ccattataca
    17581 taactcctat acggtcacta atatgtctca ccatagataa atcatgggct ataaataaac
    17641 aagtcaaatt cttttcttgt tgtaacttct taaataaatt tactatttgt gcttgtatag
    17701 acacatccaa agaagctata ggttcatcag ctattataaa ttctgggtct actgaaagag
    17761 ctcgtgctat gccaatacgc tgtctttgtc cacccgaaaa atcagatgga tacttatttg
    17821 cataacttct atcaagtcct actagattta gcatctcata tactttattc attctatctg
    17881 ttttattttt atagatacct tgaattttta gtggctctga aattatatct gcaattgtca
    17941 tacgtggatt taatgatgaa gtagaatctt gaaaaataat ctgcatactt ttgtttacat
    18001 ctttttttat aaatttgtat gtcttcttat cggatataca atttcctttg taaattactt
    18061 ctccaccagt agattcatgt agtcttacta tagtcttacc agttgtagat ttgcctgaac
    18121 ccgactctcc taccaatcca aatatttcac ccttatatat atcaaaactt atatcatcaa
    18181 ctgcttttac agtagtattt ttatccaaat gaaaatattg ctttaagttt tttatttcca
    18241 gcattttttc tctattcatt agaaatcacc cttccacaat ttactctaac tggtacatct
    18301 attttaggtg catctggatg taataaccaa gttgctgcac tatgtgtatc attgatttta
    18361 aacattggcg gctctttttc ataatctatt ttaagtgcat ttttattcct tattgcaaaa
    18421 gcatctcctt ttggagggtg caataagttt ggaggcattc caggtatatt atacaaatag
    18481 tcatcctgcg aatctaaagt aggtaatgaa cctaacagcc cccaagtata tggatgtcta
    18541 gggtcataaa atatatcctc cacagttcct atttccacaa tctttcctgc atacataact
    18601 gctattctat cagcaatagt tgcaactact cctaaatcat gagttataaa tattattgac
    18661 aaacctattt tatgttgtaa atcctttatt aaatcaatta tttgagcttg tatagttaca
    18721 tctaaagcgg ttgtaggttc atcagcaatc aatacatctg gattacaagc aagtgctata
    18781 gctattacta tacgttgtct cattccacct gaaaaatgat gtggaaattg tttaaatctt
    18841 ttctcaggat tatctattcc aaccaattca ataagctcta tggccctctt ttttgcttca
    18901 cttttactta taccttgatg aatgattaca gcttctgcaa tttgctttcc tattgatata
    18961 gttggattga gagatgtcat agggtcttga aatatcattg atatattttt ccctcttata
    19021 tcttccatct ctttttcaga cttcttaacc aaattacttg atttaagtaa aacctctcca
    19081 tttttaatat atccattgta tgggagtatc ctcatcaaac tcttgcatag tacagatttc
    19141 ccacaaccag attctccaac tatagctaat gtctcaccct ttttcagatt aaaagacacg
    19201 tttcttacag cctgaacttc tccttcttct actttaaaat ttacagatag attattaacc
    19261 tccaataaat gttccaaaaa ccatcaaccc ctatgctatt caaaccttag ggatatactt
    19321 ttatccctaa ggtttctaat taatttcaat actattcaat tgtccagtca gctatattcc
    19381 agaatatacc tacaccatga tgacctaaaa ctgtatcagg tgttaatcct ttgatatttg
    19441 gttttccaac atatattgca tctatataag caataaatgt gtaaggcata tccttagtca
    19501 tttctacttg gaattgttta tataacttta acttttcatc tttattttct gtttctcttg
    19561 ctttttgaag aattttatct atagtatgat tagagtaagc actgtaattt gcacctttat
    19621 ctgttccaaa tactttatat gtatggtcat ctgggtcaaa tggacttccc caacctatta
    19681 aatgagcatc ttgattagcc cagtctgttt cagttacaac tacagcttta gcatcaacac
    19741 caatttcttt taactgttgt gcacatatct tagccatatc aactctaact tggtcactct
    19801 cacctgctgt tatctcaaaa gctaacttag taccttcttt ttcatatatt ccatcagaac
    19861 ctaatttcca acctagcttt tcaatttctt gtttagcttt ttctggatta tattcaaact
    19921 tttctatatc aggattatta tatggtccca tttgtaaagg tgaatatgct ggaacaccat
    19981 gtcctaataa tacgctatca acgattgctt ttctatctat tgcataactt aaagcatttg
    20041 gcaatccttt agctttttta tctttaaaga atttagaatt aaaattgtat aatatacctc
    20101 tataatcggc tgttttcatt atatttactt taaaattctt ttcgtctttt tcaaagttag
    20161 acatatcttt aggtgttact tgagctaagt ctaattcccc tgattttaat tgcattgcct
    20221 tagccttatc atctggaaca atcttaaaga ctactttatc taatcctggt tcttttacaa
    20281 aataatcact attcttaaca agtgttatac tttgaccttt gtcccatttt tcaagtttaa
    20341 atggaccagt gcctattggc ttttgattaa attcatcagt agctatatcc ttcccttcta
    20401 aagcatgttt tggtaatatt cctactgtta aataatcaag cattgctgta tttggagctt
    20461 ttaaagtaat ttttatagtg ttatcattaa ctacatcaat ttttgttata tcttcataat
    20521 ttgatgctat ctcagaagca ttgtctggat tcattatagt ttctattgta aacttaacat
    20581 catttgctgt aaatttctct ccatcatgcc acttaacatc atctcttaaa ttaaaagtat
    20641 atgtatttgt tgcctcatca aatttccaat cttttgctag acaaggaacc accttattgt
    20701 tttcatcatg agctgtcaat ccattaaata ttaatgaatt tatttctcca tgttcatata
    20761 gtgcaggatt tatacttgta tagtcattac ttccataaac aagtaccttt ccatctttag
    20821 gcttatctgc tttcttatct ttgtctcctc cactagaaca acctgccatt agactaagta
    20881 tcattactaa acttaaaact tttaacttct ttaatttcat gatgctcccc cttttgcttt
    20941 ttataaaata tttttcaaag ctttgacccc atttatttaa atagatattt caaatacctc
    21001 agtatttgct tattttatac tcaaatgctg atttataaat tactagattt tttattatta
    21061 ttctttctta tgtaattacc tatatttgtt atacacacca atgtggtaac caaaaatata
    21121 cccggaatca gtataatcca ccatctgttt gaaagtaatg cttcctctga taaagataac
    21181 atacttcccc aagaaacaat ttctattggt aaaccaattc ctaaaaaact aagtgttgat
    21241 tcagtaccaa tagctgcacc tatatttgtt acaaccatga acattattga tgccacaaaa
    21301 tttggaagta aatgttgctt taatacatag aagaatcctc ctcccatact cttagctgct
    21361 agtatgtatt cactatttct tatttgtcta acctctgttc tcactatctt agatatattc
    21421 atccaacttg ttatacctat tactatagac atagatactg gatttgaatt tccaagtata
    21481 gcttgtacaa atattataat taatattgaa ggtatactaa gtatgatttc tgtaaatctc
    21541 atcattgcat catctacaaa ttcagatgct gaaccactta tactaccata aacaattcct
    21601 ataacagttg atattacagt ggaaaataat cctataaata aagatatttt tcctccatac
    21661 catatcatag aatatatatc tctacccatt gagtctgttc caaataaaaa agttttattt
    21721 ggagctaaat tagagcttgc taaatccata tatgtcggct catgtgtcat tataaaagat
    21781 gagaatacac ttccaattac tataattgat aaaataataa ttgatatata tggtaattct
    21841 ttaatttttc taaataagtt tttttttgtt tctacgtgtt catcttttgc tactaaaata
    21901 taattttctc ctacaatttc aaaatcacta tattgcggct tcattagtat cacctctatc
    21961 atatttcatt ctagggtcta ttttattgtt tataatttga gctaacatat tcccaaatac
    22021 tactaaagcc cctgttatta agcataaaac taataacata ttataatcat gatactttgc
    22081 actttcaaaa caaagtgttc ctagcccagg ataagaaaaa actttttcaa ctacataagt
    22141 tccgcctagt atatgaggta tcgaaatagc cattatactg atatatgatg gcattatatt
    22201 tctcaggcaa tgcttaaaaa caatagttct attatttaac ccctttgctt tacatagaag
    22261 tacataatct tctcttattt cttctagtaa tttatttctt atcatatatg tgtaatacca
    22321 taaatggctc aatacaagta ctgtaagagg taaaattaaa tgtgatattc tacttaatat
    22381 actggattct tctcccattg aataagcgcc actacttgga agtatactta agtttatact
    22441 aaatacaagt atgagtacta aagcaaccca aaatgatgga atacagttgg ttattgttcc
    22501 taatttacat atgactctat caatgagctt atcttcatgt agtgtacaaa atattcctaa
    22561 caataaggct agtacaaagg taagtatata tcctgaacca cctaatatta tagtgtttat
    22621 atagacatca tttataacag aagtaacatt ttgtttatat ttaaatgaaa ttccaaattc
    22681 tccttttgat gcattcgaaa tccactttat atactgattg tatattggtt catctaaacc
    22741 aagttttttc atagcatttt ctttttcttg tgtactcatt ctttctactc catccccata
    22801 ataagagatt aatgggtctc ctggagcaag tcttgccatg taaaaaacaa taaaagataa
    22861 taaaaacatt actaaaataa actgcaacaa ctttttcagt atatccttta acaaacccca
    22921 accccccttt gttgcaataa aaaaaaccac ataaaagcaa acgtaaagta acgttaaaac
    22981 cttcgtggtt ctattatcat taaacaaatt taaattttca atatttatct atgagttaat
    23041 tttagcatca tttattaact gtgtaaacaa actttttaaa aattgttaaa atttcatcaa
    23101 attattttat ttatatctaa aataaaattt ttatgttcta ttcttcatta ttttatatga
    23161 aattataaaa ttttaggttg actatataat aaaagtatat tatatttagt ttgtatgcaa
    23221 accatttaag aggtgatttt attgaaaact aattatgact gttttagaat agccatgctc
    23281 ttaaaagaac tttattctaa aactatgtac acagtggaag aaaattttaa agaaaatgga
    23341 ttaacacacc aacagattat agttataaag ctaatagctc ataatcagga acttacaata
    23401 tcacaacttt gtgatgaaat gtctttggct aaaggtacgg tttcaggcat aatcagcaga
    23461 ttagaacaga ttggatatat agaaaaattc aagaaatcta atgataaaag aaatacatac
    23521 gttaagttta caacaacagg atttgaattt gcaacaaatt ttaaaattaa gatgcaagaa
    23581 agttttgatg atatttttaa aaattgtgat gaaaaagaat tgagtgactt agtaaaaaat
    23641 cttagaacta tattggcaaa agtaaaggag agataattat gaattataaa ttaatacttg
    23701 caatagtatt tataacatca gctttaattt tctacactat aggtgtattt ggagaaagaa
    23761 aggctaaaat tttgaaaaag aaacatgtaa ttatattttg gcttggtttt atatttgaca
    23821 ctttaggaac atttacaatg agtaacatag ccaacagcca tacttttgaa gtaaaatccg
    23881 ctttatcaca aaaccttcat agtattacag gtcttcttgc aattgtgctt atgctttttc
    23941 atgcaagttg ggctactttt gtcctataca aaaatgatga ggagaagaaa aaattctttc
    24001 ataagtttag tatagttgta tggactatat ggttaattcc atactttata ggtatgttca
    24061 ttggtatggc tggatagcaa aataaaaagt gtgtaagaaa tatggtaggt aaatttgtat
    24121 aaaacaaatt tatctacacc tttctttcac acttttacat atttatctat aatacttcat
    24181 tatgtctttc atatttcttc atacctaata tagaatattt aattattcct ataatatttt
    24241 attattttct cacaattttt catatctata tggaatattt aattgtttct ataatgtttt
    24301 attatctttt tcatattttt atatataacg tttcactatt tttttcatac ttctttatta
    24361 aatatttatg tataacatat atttaataaa ctctaactgt taaacaccta atacttgagt
    24421 tactcctgga acttcactca ccaaaacatt ttcaataata gctttgattg tcatagtcgc
    24481 tcctggacac ccactacatg ctccttgtag tctaactaaa actacaccat tttcatttac
    24541 atctataagc tctacatctc caccatctct ttgcaatact ggttttatct tttcttcaag
    24601 cactttttca actttttctc tcatagctat tccccccaga aatattttat taccaaaatt
    24661 ataccaatgg tttaaatttt tgtccatcta atgatttatc aaattcgatt tttgcttcat
    24721 caataatttt agtatccata aatacatcat aggcactaca agccaatact ttagctgaat
    24781 ttatcatagc agaaaatcct atatttgaac ctgctgatga acatgactgc catgtatgac
    24841 ttgcaattcc aacaggccat gctgccattg caaattgagc cactggtata acataactta
    24901 catctccaac atcagtagaa ccagctagtc ctttatcttt gtctccatca actatacctt
    24961 tgtgtatata gctttctaca atggatttat cattttggaa tgatgttgct acacctaatc
    25021 tttcttctct agtaagagta tcacataatt tttttgcaaa ttcttcttct tcaggagtag
    25081 ttttaggaca tccaacaaaa accatattat tataaaccaa atcagtcaaa cattggtttg
    25141 gtatataatc atatattcca tctgtaacct tatactccat ttcagtttct gtcatcattg
    25201 ctgctccttg agctactttt attaatctat caagaacgtg ttctgcgtcc tttgcttttt
    25261 ttcctctaat aaagtaccaa ctttcagcaa agccaggaac tacattaggt cttccaccac
    25321 cattagttat aacatagtgc attcttatag agtcaataac atgctctctc aaataattag
    25381 ctccaacatt cataatttca acagcatcaa gtgcgcttct accattatga ggagcttgag
    25441 ctgcatgggc tgttatacct ttaaatttaa attttacaga taagtttgct agacttccac
    25501 ctctccaagg tgcattt
//
LOCUS       DAFNNB010000040        25411 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0040, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000040 DAFNNB010000000
VERSION     DAFNNB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 25411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0040"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            28..732
                     /locus_tag="L3M60_001458"
     CDS             28..732
                     /locus_tag="L3M60_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226136.1"
                     /translation="MSKLIYIADDEDNIRNLVKTFLKNEGHDVMDFKTGDELLEQFNI
                     KECDLVILDIMMPGSSGFEVCTKLREKSTVPIIMLTARDTDIDYITGITLGSDDYFTK
                     PFSPMSLVMRVKSIFRRIEFEKKQNYDKYSNLVNMELKFGDVIINKKNKIVTSKNVNI
                     DLTPNEYNLLTYLFENIDRAVSRDELLNKVWGYDIEVETRAADDTVKRLRKKILDTNI
                     LIETVWGFGFRLKEKS"
     gene            734..2221
                     /locus_tag="L3M60_001459"
     CDS             734..2221
                     /locus_tag="L3M60_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6226137.1"
                     /translation="MKNGNDKNIIGKIKSKFKLKFSKKNISIKTRILLSILCLIIIVF
                     MIILVSFNVFLDTYIKTTANEELTKSTETVEHMDSNFRPIKPPQDDGKLPPKVLSNFM
                     RNVQDKVRMAETQSDADAMVVDSKYNLIFPTRENDFLKNVDEMELIRECIQNEKLDLN
                     SDENTRISTRNKDYYISTVKITSIYGEQDKYLVLFIDISKTLNLAQKINIVLISVMCF
                     AGILAIFTAVILSEKIAKPIKELCEFAKKMGQGDFKRTYFDFSDKELVELSNVMNKSA
                     EYLDKYDNEQKIFFQNASHELRTPLMSIKGYAEAIKYNVIDSKYASDIILEESDRLSD
                     MVEDLLYISKIDNITKDFELVECDLREVLSNCGARQNVRAINKGIKFIYEFDEKMVLF
                     ECDEKNISKAFMNLIENALRYAKSEIKIVCKHNQKNIVVIVEDDGIGIKKEDLPHVFE
                     RFYKGVGGNHGIGLSIVKSIINKHGGRIYVENGKKGAKFTILFKL"
     gene            2426..4039
                     /locus_tag="L3M60_001460"
     CDS             2426..4039
                     /locus_tag="L3M60_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6226138.1"
                     /translation="MKEQLIFNITNLVTVVFEAFVIHMFLSDFLGQKEGYIKVVRYAK
                     LGFIICLGLCNLITLNPKITMPLIFILIFSTSFLYKGNLKTRLFTTVLLSIFFILSEI
                     VVTSIFVLFIKEGFEIMFENNSIRVLATILSKIVFLLTCKIICLFKKDVHLDMPIKYW
                     FPLFLIPIFSLFLSVSVFDVSKFFSLECLKFLSLISSIGILYINFIVFYLFKFIIDKT
                     KLSMKYEILEKEIIHKEELRLSNERYKIIVEQTDSVVFEWNIKENKSFVSQAWNEKFG
                     YNNVCKNIFKEIKDKDLVHSEDNAIFENFLESIKKKNRHNQAVYRLKKSNGEYIWCRT
                     SITSIYNDENELLRVVGVIVDVDSDIKKYEELKTRAESDSLTNIYNKGTFEKLVEETI
                     VMNTGDKKDALFIIDLDDFKEINDNFGHPFGDFVLKTFADKIQASFGSRDLVGRIGGD
                     EFVVYMQDYVTEVNLHKKAKELNRVLSDNYTDLSFSFDASVSIGIARYPQDGTSFFEL
                     FKNADRALYSIKASGKNSYCLFEEELYVQ"
     gene            complement(4254..5231)
                     /locus_tag="L3M60_001461"
     CDS             complement(4254..5231)
                     /locus_tag="L3M60_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6226139.1"
                     /translation="MIQCEIADRNSDKKINTLEVIIFFVVTYGISLLFGIPLYFKKYM
                     NPQIMVTFMTILPASGVAITKIYKHKKSDEHIILHCVIIADFLLSLFLVTLKAFGVID
                     DETILLGMGILVLFTGIITICYACLEGKELYPFKNVNKSIICIFIFIIIINLSKCIML
                     GWKDINFINLIMNIFLIFPYIANQSILFFGEEYGWRGFLQEKMQTKFGKRMGVILLGI
                     IWELWHIPLWFTVYHVDVLGILLRLFSVISLSIFFGYVYMKTKNVWSCVVIHLINNTV
                     LLDTDLNNAFLNFNASSLNSSIILGCLIFSVLLSLFIFSKEYKKEQLRY"
     gene            complement(5248..6078)
                     /locus_tag="L3M60_001462"
     CDS             complement(5248..6078)
                     /locus_tag="L3M60_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6226140.1"
                     /translation="MSQVPKNYFTTGEFAKICGVNKKTLFHYDDIGLFSPELKKENGY
                     RYYSYHQLSIFGIISSLKEIKMPLKEIKTYIDKRTPNLLIELLEKKTIDIKNEIEKLN
                     NIQALMESTISFTKNACNIDANTITLKEHEEEYLVKTPITYKEQFLGDEEENFLYECI
                     NFIDNYELSDYGMIGSIIKGEDIINKDFESYSYLFTKVNKEYKKYPVSIKQKGLYVTA
                     YHKGSYETIYKTYEKLLNFFSQNNLCIGDFVYEEYLLYDISVRDSNEYLTQISAEVKI
                     "
     gene            6151..7878
                     /locus_tag="L3M60_001463"
     CDS             6151..7878
                     /locus_tag="L3M60_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226141.1"
                     /translation="MKKLIHFLKPYRVLIVVVLIFTFLQTLGTLYIPTLTANIVNNGI
                     VKGDIDYIVKTGLMMIIVAGITALSAVLVCKVSANLSSGFCRDIREAVFIKSQDLSIN
                     DFNNIGTASMITRSTSDITLIGQSVFMFIQLVLPAPIITVSGLFLAYSIDKVMTIIIV
                     VVMFLFMLSAFLVGKRLIRLFKIMQIKMDNMNRVLREVVTGVRVIRAFNRSHFEKKRF
                     DKTAIDYSETAISINKVFSVLMPIVMLIMNLGIVSIIWFGGMRVSNGNMEIGHIMALV
                     EYCILILFYLIMGVMVFMYIPRAGACADRVNQILDIEPEIVDGNVYKDDVSERGHLVF
                     KNVTFSYAQSEEPVLNNITFEAKSGEVTAIIGSTGSGKSTIANIIPRFFEIQSGEISL
                     NGQDIKKIPQKELRDKIGFVPQKAFLFSGTIEENIRYGKEDASIEEVKHAASIAQADE
                     FISDMEDKYDSFVAQGGNNLSGGQKQRISIARALVRKPEVYVFDDSFSALDFKTDKRL
                     RKALKNEIKDSSAIIIAQRISTIMDANQIIVLNDGKIVGIGKHKDLLENCEVYKQIAD
                     SQLSKEELA"
     gene            7916..9778
                     /locus_tag="L3M60_001464"
     CDS             7916..9778
                     /locus_tag="L3M60_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226142.1"
                     /translation="MSEKRTDKQDIEVLSFEDGMSAEKAKDAKKTTKRLLKYMAKQKF
                     KILIIFISVLISSALTVLAPMVMGKAIDQLFNGIKTAVQTGTKFSVNFSTMGGIVSIL
                     LGLYLVSAIFIYIQQYIMSGVAQNLVLSMRKDLSDKLNKLPLKYYDSHKKGETLSIVT
                     NDLEKVADSLQEGLMQLITAVVTVVGSIVMMISISVPLTIVSAITLLVSLGITVVIAR
                     KSQERFSENQKALGELNSNIEEIFTGQIVVKAFSKEKDTIRNFKILNQNLYNASRRAQ
                     FSSYAISPIIRFINLIGYVIIAVVGGIFASTGAMTLGSIQAFIQYVNQASEPTTEISY
                     IVNMLQAAIASAERVFTVMDEVEEIKDEESSKVISMPRGKVQFEHVKFGYSDDFILMK
                     DININLNAGDKIAIVGPTGAGKTTLVNLLMRFYEIQGGRITIDGVNIKDLKRGELRTM
                     FGMVLQDTWLFNGSIKENIAYSKSDATMDEIVRAAKSARVDHFIRTLPQGYDTILTED
                     ASNLSQGQKQLLTIARAILSDPSVLILDEATSSVDTRTEVEIQKAMNNLMKGRTSFVI
                     AHRLSTIRDADLILVMKEGTIIEQGSHEELIEKKGFYEELYNSQFSSEYDEDVV"
     gene            10109..10798
                     /locus_tag="L3M60_001465"
     CDS             10109..10798
                     /locus_tag="L3M60_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HBN6226143.1"
                     /translation="MDKKLNYRFEMKHKITEADVLALKSRLYPIMKKDKNALKDGKYL
                     IRSLYFDTPEDKALLDKLNGVALREKFRIRFYNNDCSYIRLEKKIKHYNMTSKLSASL
                     TKTEVIKILNNDIEFLKESTNCLLREFYLKIKCERLEAKSIVDYDREAFVYPVGNVRV
                     TIDSDIKTSVNSIDLFNKHLPTVSIIDENMTVLEVKYDEFIPDFIKDLIQINKTTSTA
                     VSKYASSRLYI"
     gene            10885..11571
                     /locus_tag="L3M60_001466"
     CDS             10885..11571
                     /locus_tag="L3M60_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HBN6226144.1"
                     /translation="MILIMTFNDIFKSSFIENVSGFSFVDSALALGSAFLVGLFIYMV
                     YKKTYMGIMYSRPFNVSLVALTMLTTFVILAVTSNVVLSLGMVGALSIVRFRTAIKDP
                     MDLVFLFWSLGSGIVLGAGLIPLAVMGSIIMGLILIFFSNKTISETPYILMVNCNNED
                     SEDIATDKIKKAFTKYQIKSKSVTPEKGIELVFEIRMKDGETSLINDLSQVDGVTNAV
                     LVSYNGDYVA"
     gene            11588..13453
                     /locus_tag="L3M60_001467"
     CDS             11588..13453
                     /locus_tag="L3M60_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HBN6226145.1"
                     /translation="MKDKKFTLLISIMIIFLCAVVGIYSTSGNKGVDLYSDVYIEKYF
                     NRDKVMEVNIEIAESDLKDMNENAIKEEFKVAKVTIDGDTYGNVGIRTKGNSSLTSVV
                     NSDSNRYSYKINFDKYNTSQSMEGLTQLNLNNCYSDPSYMREFLTYSICEEMGLSTPE
                     FAYAKVSINGEYHGLYLAVEGLKESYLENNFGDVTGDLYKSDEGSSLQYKGDDPENYS
                     NLIVESDKKTADWSKITKLLKSLDTGEDIEKYLDVDSVLKNIAINTALLNLDSYQGSF
                     AHNYYLYEQDGVFSMLPWDFNMSFGGFSGFGGGSQSIAIDEPTTGNLEDRPLISSLLK
                     NETYKTKYHKYLEEIVTKYLDSDYLENMTTKLYNLIASYVKEDPTAFYTYEEFEKNIT
                     SSIEDSSDNKGFGNKGFDRNNSNNNSGNKSDEKEVTAELTLSEVKTNMDNETENNTTG
                     DSKNKNNTNDDKSGNGNNQKPEGSMGKGGKSIPGVLEVAKDMSKTIKAQLNGETSSTK
                     QNSGDESSSENKGTEKFDEDVGGMPEPPEGMDGKMPPGMGNMDNGDMNGKNGNMNRNQ
                     GNPREAGGFENKGGGSMSKTRTYSKLILGGASMIIMLIMLVGVSNINRRRFIKSK"
     gene            13835..14524
                     /locus_tag="L3M60_001468"
     CDS             13835..14524
                     /locus_tag="L3M60_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226146.1"
                     /translation="MLQKVLWKFLKLVFGLFICSVGIVLTINCNLGLSPWDVFHQGLS
                     NHIGITIGTASIIVGSIVVIADVVLGENVGWGTVFNMLLIGFFMDLLLYSNLIPEADS
                     LFVGIIMLILGLILLAVGMVFYMGSGLGSGPRDGLMVALQKKTGKSINLIRGIIEVGA
                     LIVGFLLGGKVGIGTIISAFGLGYFTQMVFGLFKLDCSKIKHRFIVDDIKFIKVYMSG
                     EKKAATQNIVK"
     gene            14960..15499
                     /locus_tag="L3M60_001469"
     CDS             14960..15499
                     /locus_tag="L3M60_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226147.1"
                     /translation="MDIGEKIKRLRTEKQLTQEELANRCELSKGFISQLENNLTSPSI
                     ATLIDILEILGTNLREFFNEIDDERISFTKEDMFETEDEDLKYKLKWLIPNSQKNEME
                     PIIITLYPGGQYKEEKPHEGEEFGYVLAGSIYVHIGEKKNKVKKGESFYFRPKANHYI
                     SNEGKTTAKVIWVSTPPSF"
     gene            15518..16561
                     /locus_tag="L3M60_001470"
     CDS             15518..16561
                     /locus_tag="L3M60_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226148.1"
                     /translation="MVENIIELRDISKHYEELTVLDNFNLDIKKNEFLTLLGPSGCGK
                     TTTLKIIAGFEYADDGKVLFEGKEINNLPPYERQVNTVFQKYALFPHMDVYENVAFGL
                     KIKKTPKDVIDAKVKEVLKLVALEGFERRQVESLSGGQQQRIAIARALVNEPKVLLLD
                     EPLGALDLKLRQEMQIELKRIQKKLGITFVFVTHDQEEALTMSDTIVVMYKGKIQQMG
                     TPQDIYNEPKNSFVAKFIGESNIFDGIMIEDYKVNFCNRDFECVDKGFEKNENIEVVI
                     RPEDIKMVKPEEGMLKGKVTSTVFKGVHYEIELNENGRLWILHNTKNAEVGTELGMDI
                     YPEDIHIMRKESN"
     gene            16563..17399
                     /locus_tag="L3M60_001471"
     CDS             16563..17399
                     /locus_tag="L3M60_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226149.1"
                     /translation="MKRKSFLAYPYVVWSAIFVIIPLILVVFFSFTKESGGGYAFTLE
                     NYKEVIDPIYIKVFGRSILLAGGATLICLVVGYPVAYIISKARVSRRGSLILLFILPM
                     WMNFLLRTYAWVAILGKNGLLNTFLGWFGIQPLAILYTNFAILLGMVYNFLPFMVLPI
                     YTALSKMDNDLINAAHDLGANNMTVFRKIIFPLSLPGVMSGITMVFMPAVTTFAISRL
                     LGGGKIMLVGDLIEQQFTVVGDWNFGSAISIFMMIVILISMSIMSKFGDESDKEGGGL
                     LF"
     gene            17504..18178
                     /locus_tag="L3M60_001472"
     CDS             17504..18178
                     /locus_tag="L3M60_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226150.1"
                     /translation="MARWNGFTWKWYGQLLQNESIMSALYYTIVIAILASVISTIVGT
                     ISAIGIHKMRGKSKKLILNVNYLPILNTEIVTAVALMSLFVFVKMEFGFTTMLLAHIM
                     FCLPYVILSVLPKMKQLPDNIEDAAMDLGATPIYALRKVILPQIKPGIVSGFLIAFTM
                     SIDDFIISFFNAGNGVSNLSIEIYGMARRGIKPEINALSTIMFAVVLGLLLLANKKES
                     IVRGTK"
     gene            18178..19230
                     /locus_tag="L3M60_001473"
     CDS             18178..19230
                     /locus_tag="L3M60_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6226151.1"
                     /translation="MIKFKKAISLIAVGMMTVTLFTGCNKATDSTKVLNVYNVGDYID
                     ESLIDKFEKETGIDVQYSTYDTNEMMYQKVKSGSTHYDLVFPSDYMVEKMKNEGLLEK
                     LDFKNIPNMKYLDKSFLNPVYDKTNEYSVAYMWGTLGILYNKKEVKDPIDSWNILWNP
                     KYKGNIMMFDSVRDTIGITLKKLGYSMNSVNPKEINEAKNLLMKQKDLVLAYVNDEGK
                     DRLLGGEVAMGMLYSGDAVTLMEQNPDLDYAIPKEGTNKWVDAMCVPTTAQNKKEAEL
                     FINFLLDPENAKVNAEYIGYSTPNTGALKLLDPEITENPVAYPSKEVLNKCETFEDIG
                     ENIKLYDKAWIELKSK"
     gene            19489..20337
                     /locus_tag="L3M60_001474"
     CDS             19489..20337
                     /locus_tag="L3M60_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6226152.1"
                     /translation="MNIVNKKIDLLILALLVFIFLIVGIVILSIRTENKLNVFIMLAI
                     IFFIIMLTYLSNSIVGLITSSIIIFMYTSYILYNNITYNMDVEFISYMWIIAIPISSI
                     IMGNLNKSINELQNTNKKLSEQYKELVTIDSETGLRNLKIFYNDVNMEISKSIRHNTD
                     FSLMIVKLPYYGNLQTIFGESKTNKIVKYIGSNIIECTRNEDIIYSLQKDMLGILMPN
                     TSLEGSKVVKDRIKKRIKELNLDLNNRGKYVNIDVKIAFLQYRDSFGDSINFKNIVEE
                     ELQYDV"
     gene            20327..21991
                     /locus_tag="L3M60_001475"
     CDS             20327..21991
                     /locus_tag="L3M60_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6226153.1"
                     /translation="MMFKIFNKSLLKLVAILSIVLIISNFSLSSYASEITSRTDNVLI
                     VYDSKKETDYNRDILNIMRTLLGRFSSDIKLLKLSNYDGEINKNYYSHIFILGINENS
                     YDNDNKTKNLISSLNLYKGTICWLGYGIENLLEHKKYNLDYIGKTNNIVSVNYRGKSY
                     NLEEHYIFNIVESKDTSNKVIGSINDTLNKYPYIINDKNLFYVSKLDLDGVLFYIFCD
                     SLNDIFNIKRFDKGRIFVRIEDVHAFRDPKDLVNIADYLSSKDIPFTIALIPAYVNPK
                     NHKITTLSESPEIVKAIKYMQDKGGTVILHGYTHQYKKEEVSGEGYEFWDGKKDEPLK
                     ENMKIFVKDRVLSGLRVCIENGIYPLAFEAPHYAMESDGYKELKKYFSTYMGQHQNNN
                     KKFSTNTYPYIIRDTEEFNILIPENLGYIDPEDKFTFQHIKENLDKLSIVRGFSGGFF
                     FHSYLNIEYLKNTIEYLEKQNIEFMNLRDFNNWVKVDEIQIRNNGDEIIVNYDRDLEE
                     NIKSDIRFKSISNISKILIFIVSISVLIFMIIFIYFKRIDKKKFLK"
     gene            22040..23296
                     /locus_tag="L3M60_001476"
     CDS             22040..23296
                     /locus_tag="L3M60_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003362983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6226154.1"
                     /translation="MRDLNVSECLFVFSLCSIWSLLLINIILAMGGYIFYFKNFDKEI
                     KEIDEYPMVSILVPAHNEAKVIGRTVESLLLLNYPKSKMELIVINDNSSDNSKEILED
                     IKNKYNNYNFTIINTDSLTGGKGKSNALNIGYTISKGDFIAVYDADNTPDRNALRYLV
                     QTIVMNDELGAVIGKFRTRNKNKNLLTKFINIETLSFQWMSQAGRWQLFNLCTIPGTN
                     FILRRSIIEEIGGWDSKAIAEDTEISFRIYKLGYKIKLVPQSITWEQEPETVKVWIKQ
                     RTRWAKGNIYVLMKYIKNIFKQGRNKIVFDIAYFFSVYFLFLTSVIISDILFVLSISK
                     LVEISIPINFFLIWILSYLLFIIEVSISLTIEKGEATIKNIFIVAIMYFTYSQLWLFV
                     AIKGMIEYLKDIIFKREVKWYKTERF"
     gene            23319..25400
                     /locus_tag="L3M60_001477"
     CDS             23319..25400
                     /locus_tag="L3M60_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBN6226155.1"
                     /translation="MKKFIISIISLVLFFSNISLIDKVNADETKVKNYKFERDITIDG
                     VIGSNSTFFEVNKNWDIEDVLLHLNFSKSQILNGDVSSLTVLINNVPIKSIKLNAKTN
                     YKNTLEVLVPKDYIIQGYNEIKVKTYKTISDKICQDDSNTGNWMVIHKESYISIRYKQ
                     KKMENSINEYPYPYAEIENNLKLDTTIVVPDNMTRGESTAVFNLASEFGKIIKNDDLK
                     LDVKLYSEMKNWSDDNIIYIGKPENTAEEILDILSTEEQTLLSSNCLIKQVDSPYNKN
                     KKMMVVIGSNEENLIKASNLLIENRLSNQVLSSSVLVNKETNIKINREQKLNLGHLTL
                     KDLGYSDFLLEGAFNQQALFDVKIPTGKVLDDGSKIVLNLRYSDNLHFEKSLVTVSIN
                     DVLVGSKKLDRQHSNNDKLELKIPKDIDNKNYYQVKLVFNLNIKNSNCVTRESNNPWA
                     YVSNSSYLALSTKENEALSFESYPYPFVRNDEFNDLTVIMPDYSDSQAMTWMSKLGVT
                     LGANINSHNGNMNVIRGKEFSDKYKDTNIIVFGVPHNNSVIKMLNNNLNIKFDKNYSN
                     FLSNDKLSFIDDYGKNISTIQLIKSPYNNQKNIMVISSMNEKNLYLGMDYLLNKSKVN
                     DLKGDTLIIDEYGEVEDLAYNLKPKKEVKNSSWNMSINKTTKVFLMISFITIIVVIIL
                     SMLYIKKYKRR"
BASE COUNT         9992 a         2576 c         4330 g         8513 t
ORIGIN      
        1 ataaaaagtc tattaaggag tgagcttatg agtaaattaa tttatatagc agatgatgaa
       61 gataatataa gaaatttggt aaagacattc ttgaaaaatg aaggtcatga tgttatggat
      121 tttaaaacgg gtgatgaact tttagaacaa tttaatataa aagagtgtga cttagtaata
      181 ctagatataa tgatgcctgg ttcaagtggt tttgaagtat gtacaaaact tcgtgagaaa
      241 agtacagtcc caataatcat gcttactgca agagatacag atatagatta tattacaggg
      301 ataaccttag gtagtgatga ttattttaca aaaccattta gcccaatgtc tttggttatg
      361 agagtaaaat ctatatttag aaggatagag tttgaaaaaa aacaaaatta tgataaatat
      421 agtaacttgg taaatatgga attaaaattt ggggatgtaa taataaataa aaagaataaa
      481 atcgttacaa gtaaaaatgt aaatatagac cttacaccaa atgaatataa cctattgact
      541 tatttgtttg aaaatataga tagagcagtt tcaagagatg aattattaaa caaagtatgg
      601 ggatacgata tagaagttga gacaagggct gctgatgata cagtgaagag attacgtaaa
      661 aaaattttag atacaaatat tttaatagaa actgtatggg ggtttggatt taggttgaag
      721 gagaaaagtt aagatgaaga atggaaatga caagaatata attggaaaga ttaaatccaa
      781 atttaaatta aagttctcaa agaaaaatat aagcataaaa actaggattt tgttgagcat
      841 actttgtttg ataattatag tatttatgat aatattagta tcttttaatg tatttcttga
      901 tacatatata aaaacaactg cgaatgaaga attaacaaag tctacagaaa cagtagaaca
      961 tatggatagt aattttagac ctattaagcc accacaagat gatggaaagc ttcctcccaa
     1021 agtattatct aattttatga gaaatgtaca agataaagtt aggatggcag aaacacaatc
     1081 agatgctgat gctatggtag ttgattctaa gtataacctg atatttccaa caagagagaa
     1141 tgatttctta aaaaatgttg atgagatgga gctgattagg gagtgtattc aaaatgaaaa
     1201 attagattta aattcagatg aaaatacacg gatatctacc agaaataagg attattatat
     1261 ttcaactgta aaaattacta gtatatatgg tgaacaagac aagtatcttg tactttttat
     1321 agacatatca aaaactctaa atctagcaca aaagataaat attgtattaa tatctgtaat
     1381 gtgttttgct ggaatattag caatatttac agctgtgatt ttatctgaga aaattgctaa
     1441 accgataaaa gagttgtgtg aatttgcaaa aaaaatgggt caaggagatt tcaaaagaac
     1501 ttactttgat ttttcagata aagaattagt agagctttca aatgttatga ataagagtgc
     1561 tgagtatctg gataagtatg ataatgagca aaaaatattt tttcaaaatg catctcatga
     1621 attaaggacg ccactcatgt ctattaaagg ttatgcagaa gctataaaat ataatgttat
     1681 agatagtaag tatgcaagtg atataattct tgaagaaagt gataggttaa gtgatatggt
     1741 tgaagattta ttatacattt ccaaaataga taatataact aaagattttg aacttgtaga
     1801 atgtgattta agagaagtac tttcaaattg tggagctagg caaaatgtaa gagctataaa
     1861 caagggtata aaatttattt atgaatttga tgaaaaaatg gttttatttg aatgtgatga
     1921 aaaaaatatt tccaaagcat ttatgaatct tatagaaaat gctttgagat acgctaagag
     1981 tgaaataaaa atagtctgta agcataacca aaaaaatata gttgtgatag tagaagatga
     2041 tggaattgga attaagaaag aagatttgcc tcatgttttt gaaagatttt ataagggagt
     2101 agggggaaat catggaattg gattatcaat agttaaatct attataaata agcatggtgg
     2161 acgtatatac gtagaaaatg gcaaaaaagg agctaagttt actatattat ttaaattgta
     2221 aaaaaattag agaaaatata gagatgtttg aagttgtttt ttatatatat ctgttgtata
     2281 ctgttgaaaa aagaacaata ttaagctgac atatattata ataattgaca tttaaagtaa
     2341 atgcttaaat atagatacga aaggaaaagt aaaatctgaa gtgcatttag gtttaagtaa
     2401 acttcttatt ttataaaaaa tatgtatgaa agaacagctg atttttaata ttacaaattt
     2461 agtaacagtt gtatttgaag cttttgtcat ccatatgttt ttatcagact ttttaggtca
     2521 gaaagaaggg tatataaaag ttgtaagata tgctaaattg ggttttataa tatgtttagg
     2581 actttgtaat ttaataactt taaaccctaa aattacaatg cctttaattt tcattcttat
     2641 tttttcaaca tcatttttat ataaaggaaa tttaaagaca cgattgttta cgactgtttt
     2701 attaagtata ttttttatac tgtcagaaat tgtagttaca agtatttttg ttttatttat
     2761 aaaagaaggc tttgaaataa tgtttgaaaa taatagtata agagtattgg cgacaatttt
     2821 atctaaaatt gtctttttgt tgacttgtaa aattatttgt ctattcaaaa aagatgtaca
     2881 tttagatatg cccataaagt attggtttcc tttattttta atacctatat ttagtttatt
     2941 tctgtctgtt tctgtctttg atgtaagtaa attttttagt cttgaatgtt taaaatttct
     3001 gagcttaata tctagtattg gtattttgta tattaatttt attgtctttt atttatttaa
     3061 atttattata gataaaacta aactatctat gaaatatgaa atacttgaaa aagaaataat
     3121 tcataaggaa gaattaagac ttagtaatga gcgttataaa attattgttg aacagaccga
     3181 ttctgttgtt tttgaatgga atatcaaaga aaacaaatcg tttgtatcac aggcttggaa
     3241 tgaaaaattt ggatataata atgtatgtaa gaatattttt aaagaaatta aagataaaga
     3301 cttagttcat tctgaagata atgctatatt tgaaaatttt cttgaaagta ttaaaaaaaa
     3361 gaatagacac aatcaagcag tatatcgttt aaaaaaatct aatggtgaat atatttggtg
     3421 tagaacatca attacaagta tttataatga tgaaaatgag cttttaagag ttgtcggagt
     3481 aattgtagat gtagatagtg atataaaaaa atatgaagag ttaaaaacta gagcagaaag
     3541 tgattcattg accaatatat acaataaagg tacatttgaa aaattggtag aagaaactat
     3601 agttatgaat acaggtgata aaaaagatgc attattcatc attgatttag atgattttaa
     3661 agaaataaat gataattttg gtcatccatt tggtgatttt gtactgaaaa cctttgctga
     3721 taagatacag gctagttttg gtagtagaga cttagtcgga agaattggtg gagatgaatt
     3781 tgtagtatat atgcaagatt acgttacaga agtaaatttg cacaaaaaag ccaaagagtt
     3841 aaatagagta ctttcggata attacactga tttgagtttt agttttgatg cttctgtcag
     3901 tataggtatt gcaaggtatc ctcaagatgg aacttcattt tttgaattgt ttaaaaatgc
     3961 agatagagca ctttatagca taaaagcatc aggtaaaaat tcatattgtt tatttgaaga
     4021 agagctatat gtacagtgaa tttagatttt atgtgatatc aagatttata tatgcttgtg
     4081 gcatgtctaa tttataaaat atgttactat gtatgttttt atatgtttca gtgaggttaa
     4141 ttaatataag caaaggtgta agtaaattta tattaatgta agtttattta caccttttta
     4201 aaaataaatt ttgtaaattt aaacagttaa ttttataatt atttttattt attttaatat
     4261 cttagctgtt cctttttgta ttccttagag aaaataaaca atgaaaggag tacagaaaaa
     4321 attaaacatc ctaatataat acttgaatta agacttgaag cattaaaatt taaaaatgca
     4381 ttatttaaat cggtatccaa aagaacagta ttattaatta aatgaattac tacacatgac
     4441 catacgtttt tagttttcat atagacataa ccaaagaata tagaaagact aatcacagaa
     4501 aataatctaa gtaatatacc taaaacatct acatgataaa ctgtaaacca taatggtatg
     4561 tgccataatt cccatataat ccctaataat ataacgccca ttcgtttgcc aaattttgtt
     4621 tgcatttttt cttgaagaaa acctctccac ccgtattctt ctccaaagaa aaggatactt
     4681 tgatttgcta tatatggaaa aattaaaaat atattcataa tcaaatttat aaaattaata
     4741 tctttccatc ctaacattat acatttactt agattaatta ttattataaa aataaaaata
     4801 caaataatag atttatttac attcttaaat gggtaaagct ctttgccttc taaacatgca
     4861 taacatatcg ttataattcc agtaaataag actaaaatac ccattcctaa gagtatagtt
     4921 tcatcatcta ttactccaaa tgcttttaaa gtaactagaa atagagataa taaaaagtca
     4981 gctattataa cacagtgaag tataatatgt tcatcactct ttttatgttt ataaatcttt
     5041 gttattgcaa ctcctgatgc tggtaatata gtcataaatg ttaccattat ttgtggattc
     5101 atatattttt taaaatatag aggtatccca aatagaagtg atataccata agttactaca
     5161 aaaaatatta taacttctaa agtatttatt tttttatcac tatttctatc agctatttcg
     5221 cattgtatca taataattta tctcctttca gattttaact tctgctgaga tttgagttag
     5281 atattcatta gaatctcgca cacttatatc atatagaaga tattcttcat aaacaaaatc
     5341 cccaatacat aaattatttt gactaaaaaa atttaataac ttttcataag tcttgtaaat
     5401 agtttcataa ctacctttat gataagcagt tacataaagc cctttttgct tgattgatac
     5461 aggatatttc ttgtactctt tattaacttt tgtaaataaa tatgaatatg attcaaaatc
     5521 tttattaatt atatcttcac ctttaatgat tgagccaatc ataccataat cactcaattc
     5581 atagttgtct ataaagttaa tacattcata taaaaaattt tcttcctcat ctcctaaaaa
     5641 ctgttcttta tatgtaatgg gcgttttgac caagtactct tcttcatgtt cttttaaagt
     5701 aatagtattt gcatctatat tacaggcatt tttagtaaat gaaatagtag attccattag
     5761 agcttgaata ttatttagtt tttcaatttc atttttaata tcaatagttt tcttttctaa
     5821 aagttcaatt agtaaattag gtgttctttt gtctatataa gttttaattt cttttaaagg
     5881 catttttatt tccttaagtg aagaaattat accaaaaatg ctaagctgat ggtatgagta
     5941 gtatctatat ccattttctt tctttaattc tggagagaaa agacctatat catcataatg
     6001 gaaaagtgtt tttttattga ctccacaaat ctttgcaaat tctccagttg taaaataatt
     6061 ttttggaact tgactcataa ttataattcc tccttgacta tcctgttaca gtatagttta
     6121 tcataatatt ttgtaaagga gtgtgattgt atgaaaaaat taattcattt tttaaaacca
     6181 tatagagttt taattgttgt agttttgata tttacgtttt tacaaacact tggaactttg
     6241 tatataccaa cacttacagc aaatatagta aacaatggca tagtaaaggg agatatagat
     6301 tatattgtaa aaacaggttt aatgatgatt atagtagctg gtataacagc tttatcagct
     6361 gtgttagttt gtaaagtatc agcaaatctt tcatctggat tttgtagaga tattagagaa
     6421 gcagtattta taaaatcaca agatttatca ataaatgatt ttaataatat aggtacagca
     6481 tcaatgatta ctaggagtac tagtgacatt actttaatag gtcagtctgt atttatgttc
     6541 attcaattag tattgccagc accaattata actgtttctg gcctgttctt agcttattct
     6601 atagataaag taatgacaat tattattgtt gttgtaatgt ttctattcat gttatcagca
     6661 tttttagttg gtaaaaggct tattagattg tttaagatta tgcaaataaa gatggataat
     6721 atgaataggg tattgcgtga ggtagtaact ggagtaagag ttataagagc ttttaataga
     6781 agtcattttg aaaaaaagag atttgataaa actgcaatag attattctga aacagcaatt
     6841 tcaataaata aagtattttc agtacttatg cctattgtaa tgttaataat gaatttaggt
     6901 atagtaagta ttatttggtt tggtggtatg agagtcagta atggaaatat ggagataggt
     6961 catataatgg cacttgttga atattgtata ttaattttat tctacttaat catgggagta
     7021 atggtattta tgtacatacc tagagcaggt gcctgtgcag atagagtaaa tcaaatatta
     7081 gatatagaac ctgaaattgt agatggaaat gtctacaaag atgatgtctc agaaagagga
     7141 catttagtat ttaaaaatgt aaccttttca tatgctcaat cagaagaacc tgttttaaat
     7201 aatataacat ttgaagcaaa gtctggagaa gtaactgcaa ttataggaag cactggctca
     7261 ggcaaatcta ctattgcaaa tataatacca cgtttttttg aaattcaatc tggagaaatc
     7321 tctctaaatg gtcaggatat taagaagatt cctcaaaaag aacttagaga taagattggg
     7381 tttgtaccac aaaaagcttt tttatttagt gggacaatag aagaaaatat aaggtatgga
     7441 aaagaagatg caagtattga agaagtaaag catgctgcaa gtatagctca agctgatgaa
     7501 tttatttctg atatggaaga caagtatgac tcatttgtag cacaaggtgg aaataatttg
     7561 tcaggtggtc aaaaacagag aatatccatt gcaagagcat tagttagaaa gcctgaagta
     7621 tatgtctttg atgatagttt ttctgcacta gattttaaga cagataaaag acttagaaaa
     7681 gcgttaaaga atgaaatcaa agattcatca gctataatta tagcacagcg tataagtaca
     7741 atcatggatg ctaatcaaat tatagttttg aatgatggaa aaattgtcgg tattggaaaa
     7801 cataaagatt tacttgaaaa ttgtgaagtt tataagcaaa ttgcagattc acaacttagt
     7861 aaagaagagc ttgcatagga tagggaatta atctgtgtat ttggaggtgt gtaatatgag
     7921 tgaaaaaaga acagataaac aagatataga ggtacttagt tttgaagatg gtatgtcagc
     7981 agaaaaagct aaggatgcaa aaaaaactac taagagatta ttaaaatata tggcaaaaca
     8041 aaagtttaaa atattaatta tttttatttc agtattgata agttcagcac ttacagtttt
     8101 agcacctatg gttatgggaa aagctattga ccaactattt aatggaataa aaactgcagt
     8161 ccaaacaggt acaaaattca gtgtgaattt tagtactatg ggaggtatag tatcaatact
     8221 tctaggattg tacttggtta gtgcaatatt tatatatatt caacagtata ttatgtcagg
     8281 agttgcacaa aatttagttc ttagcatgag aaaagattta agtgataaac tcaataaact
     8341 tcctcttaag tactatgatt ctcataaaaa gggggaaaca cttagtatag taaccaatga
     8401 tttagaaaaa gtagcagata gtttacaaga aggattaatg caacttatta cagcagtagt
     8461 tacagtagtt ggttctattg taatgatgat aagtataagt gtaccactta caatagtatc
     8521 agctataaca ttacttgttt cattgggtat tactgttgta attgcaagaa aatcacaaga
     8581 aagattctct gaaaatcaaa aggcactagg agagttaaat agcaatatag aagagatatt
     8641 tactggtcaa atagtggtta aagcatttag taaagaaaag gatactataa gaaactttaa
     8701 aatattaaat caaaatcttt ataatgcaag tagaagagca cagttttcgt catatgcaat
     8761 atctccaatt attagattta taaacctaat tggctatgtt attatagcag ttgtaggtgg
     8821 tatatttgca tcaactggag caatgactct tggtagtata caagcgttta ttcaatatgt
     8881 aaatcaagca tcagagccta ctacagaaat atcttatatt gtaaatatgc ttcaagcagc
     8941 aatagcatct gcagagagag tttttacagt tatggacgag gtagaagaaa taaaggatga
     9001 agaatcttca aaggttatca gcatgcctag aggtaaagta caatttgagc atgttaaatt
     9061 tggatatagt gatgatttta ttcttatgaa agatattaat atcaatttaa atgcaggaga
     9121 taagattgca attgttgggc ctacaggagc tggaaagaca actcttgtaa atttacttat
     9181 gagattttat gagatacaag gtggaagaat tactattgat ggtgttaata taaaagactt
     9241 aaagcgtgga gaacttagaa ctatgtttgg tatggttctt caagatacat ggttgtttaa
     9301 tggaagtata aaagaaaata ttgcatatag taagtcagat gcaacaatgg atgaaattgt
     9361 aagagcagca aaatcagcaa gagtagacca ttttataagg actttaccac aagggtatga
     9421 cactatattg actgaggatg cttctaatct atctcaaggt caaaagcaac ttctaacaat
     9481 tgccagggca atactttcag acccatcagt attgattctt gatgaagcaa catctagtgt
     9541 agatactaga acagaggtag aaattcaaaa ggcaatgaat aacttgatga agggaagaac
     9601 tagttttgtt attgcacata gattatcaac tattcgagat gctgatttaa tattagtaat
     9661 gaaagaaggc actattattg agcaagggag tcatgaggaa cttattgaaa agaaaggatt
     9721 ctatgaagaa ctttataaca gtcaattttc aagtgaatat gatgaagatg ttgtatgatt
     9781 atcaagtttt aggcaactaa atgattgata aatattaagt taaatacagt aacatataag
     9841 gttatatgga ataagagtat ttctctattt catataacct tttttatttg tcaaaataag
     9901 ataaaatttc ctttctaaat tagattgaag aatgtattta tcaatataaa agtaaaataa
     9961 cccttaatta gtgaagtaaa tattgcttgc cacatatatg ccataaagat gcacttattt
    10021 tacggagatt taacaaatta gcataaagta tactctaaat atagaataaa tcaaatagag
    10081 ttaaataaat tgagaggtga tataaataat ggataaaaaa ttgaactata gatttgaaat
    10141 gaagcacaaa ataactgaag cagatgtgct agctttaaaa tcaagactct atccaatcat
    10201 gaagaaagat aaaaatgcat taaaagatgg aaagtatctt attagaagtt tatactttga
    10261 tactcctgag gataaagctc ttttggataa attgaatggc gttgcattaa gagaaaagtt
    10321 caggataaga ttttataata atgactgctc ctatataagg ctagagaaaa aaatcaaaca
    10381 ttacaatatg acatcaaagc tatcagctag tttaactaag acagaagtga taaagatatt
    10441 gaacaatgat attgaatttc taaaagaatc gacaaattgt ttattaagag aattttattt
    10501 gaaaataaaa tgtgaaagat tagaggcaaa gagtatagtt gattatgata gagaagcctt
    10561 tgtttatcca gttggaaatg ttagggttac aattgatagt gatattaaaa cttctgtaaa
    10621 ttcaatagat ttgtttaata aacatttacc aactgtttcg ataattgatg agaacatgac
    10681 ggtacttgag gttaaatatg atgaatttat accagatttt ataaaggatt taattcaaat
    10741 taataagact acttctacag cagtttcaaa gtatgcttca agtagattat atatttaata
    10801 ataaaagcta tgatttatat gaagctatac ataaatgagg ttaaaaaata aataaaataa
    10861 aaaataaatt aaagattagg aaggatgatt ttaattatga catttaacga tatttttaaa
    10921 tcaagtttta tagaaaacgt atcaggtttt tcatttgtgg acagtgcttt agcactaggt
    10981 tcagcctttt tagtaggttt gtttatatat atggtctata aaaagactta tatggggatt
    11041 atgtattctc gaccatttaa tgtgtcgctt gtagcactta caatgcttac aacctttgta
    11101 atattggcag ttacttcaaa tgtagtttta tctttaggta tggttggtgc tttatctata
    11161 gtaagattta gaacagcaat taaagaccca atggatttag tatttctatt ttggtcattg
    11221 ggttcaggta tagttttagg agcaggttta attccattag ctgtcatggg ttcaataatt
    11281 atgggattga ttctaatatt cttttcaaat aaaacaatat ctgaaactcc atatatattg
    11341 atggtaaact gtaataatga agattcagaa gatatagcaa ctgacaaaat taaaaaagca
    11401 tttactaaat atcaaataaa atcaaaaagt gttacaccag aaaaaggaat agaattagtg
    11461 tttgaaataa gaatgaaaga tggagagaca agtcttataa atgacttgag tcaagtagat
    11521 ggagttacta atgccgttct tgtaagttat aatggtgact atgtagcata gagaggagta
    11581 atcttttatg aaagataaaa aatttaccct tcttatctca ataatgatta tatttttatg
    11641 tgctgtagtt ggaatttata gtacatctgg caacaaaggt gttgatttat acagtgatgt
    11701 atatattgaa aaatatttta acagagacaa ggttatggaa gttaatatag agatagctga
    11761 aagtgattta aaagacatga atgaaaatgc tataaaagaa gaatttaagg ttgcaaaagt
    11821 aactatagat ggagatacat atggaaatgt aggtataaga actaaaggaa attcaagtct
    11881 tacgtctgta gtaaatagtg atagcaatag atatagttat aagattaatt ttgataagta
    11941 caatactagt caaagtatgg aagggcttac tcaattaaat cttaataact gctattctga
    12001 cccatcttat atgagagagt ttttaacata tagtatttgt gaggagatgg gattgtccac
    12061 tccagaattt gcatatgcta aagtatctat aaatggcgaa tatcatggtt tgtatctggc
    12121 agtggaagga ttaaaagaat cttatcttga aaataatttt ggagatgtaa ctggagacct
    12181 atataaatca gatgaaggaa gttcgttaca atataaagga gatgacccag aaaactactc
    12241 aaatctaatt gttgaaagtg ataaaaagac agctgattgg tctaaaataa caaaactatt
    12301 aaaatctttg gatacaggtg aagatattga aaaatatctt gatgtagatt ctgtccttaa
    12361 aaatatagcg ataaatacag ctttattaaa ccttgatagt tatcaaggaa gttttgccca
    12421 taactattat ttatatgagc aagatggggt attttccatg ttaccatggg attttaatat
    12481 gtcatttgga ggatttagtg gttttggtgg aggtagtcaa tctatagcaa ttgacgagcc
    12541 gacgacaggt aatttagaag acagacctct catatcttca ctattaaaaa atgagacata
    12601 caaaacaaaa taccataaat atctggaaga gatagtaact aaatacttag attcagacta
    12661 tctagagaat atgacaacaa aattgtataa cctgatagca tcatatgtaa aagaagaccc
    12721 aacagcattt tatacttatg aggagtttga aaaaaatata acgtcttcaa ttgaagattc
    12781 tagtgataat aagggatttg gtaataaagg gtttgacaga aataactcta ataataattc
    12841 tggaaataaa agtgatgaaa aagaagttac tgctgaatta acattaagtg aagtcaaaac
    12901 taatatggat aatgaaactg agaataatac tacaggtgat agtaaaaata agaataatac
    12961 gaatgatgat aaaagtggaa atggtaataa tcaaaagcca gaaggttcta tgggtaaagg
    13021 aggaaagtca ataccaggag ttttggaagt tgcaaaagat atgagtaaaa ctataaaagc
    13081 tcaattaaat ggagaaactt cttcaacaaa gcaaaattct ggtgatgaaa gctcaagtga
    13141 aaataaaggg actgaaaagt ttgatgagga tgtgggtggt atgccagaac cacctgaggg
    13201 aatggatggt aaaatgccac caggaatggg aaatatggat aacggtgata tgaatggtaa
    13261 aaatggcaat atgaatagaa atcaaggtaa tccaagagaa gctggaggtt ttgaaaataa
    13321 aggagggggc tctatgagta aaacaagaac atactcaaaa ttaattttag gtggagcttc
    13381 aatgataatt atgttgatta tgttagtagg tgtatcaaat ataaatagaa gaagatttat
    13441 aaagtcaaaa taagattgat attaaaaaat actgttgagt agacatctac tcaacagtat
    13501 tttttagata ttaagattaa atttatttag aaataagagt tagatttttt tacttaaatt
    13561 gaaataaaat atgcaaatag ttttaaaatg tagaaattat ttataataat attaaaaaaa
    13621 atttaattga ataatattaa aaaaattgta actaaattgt aataaaatgg tagttattta
    13681 gcataaaatg atgtatcata gaagtgtatt atttaataga aatctccaga tttaaatatc
    13741 acataagata gttaatgtgg agtagttcaa gagaaaactg ctggctttcg atatttagag
    13801 tgagtagtta aattatgttt aggaggggta gaatttgttg caaaaagtat tatggaaatt
    13861 tttaaagcta gtgtttggtt tatttatatg ttcagttggt atagtattga cgataaattg
    13921 taaccttggc ttatctccct gggatgtatt tcatcaagga ctttcaaatc atataggaat
    13981 aactatagga actgcctcta taattgtggg ttctatagta gttattgctg atgtcgttct
    14041 tggagaaaat gtaggatggg gaactgtttt taacatgcta ctaattggat tttttatgga
    14101 tttactttta tatagcaatt taattccaga ggctgatagt ttatttgtag gaataattat
    14161 gttgatatta ggattaattc ttttggctgt tggaatggtc ttttatatgg gaagtggttt
    14221 aggaagtgga cctagagatg gtttgatggt tgcacttcaa aagaaaacag gaaagtcaat
    14281 taacttaata agaggtatta tagaagttgg agcattaata gtaggatttt tattaggtgg
    14341 aaaagttggt ataggaacta taataagtgc atttggattg gggtatttta cacaaatggt
    14401 atttggctta tttaaattag attgtagtaa gattaaacat agatttatag tagatgatat
    14461 aaaatttatc aaagtgtata tgagtggaga gaaaaaagct gctactcaaa atatagttaa
    14521 gtaatttaaa aataaattca acgaaatgtt aggtatttta aattaaaatt ttgtttagag
    14581 atataaacta aatattaaaa gaatattaaa atataaggat tgctagaaca ctataaaata
    14641 tagatttcta gcaatttttt atttgttcaa tgttttttta gtattagaag tcttattctc
    14701 tatttaatga atgaattaat ttataattag agatactaaa ctttatagat attaatataa
    14761 acacctttaa actgtgtatt ttccaaagcc tttaaaatgc ttgtatttat tctaaataaa
    14821 aaaataaaaa aatgtcaaaa aaactcttgt attatatcca aattttatat ataattacct
    14881 atgttgttta gaaattttaa actcaatgtt tagtaaacaa aatgtttagt aaaataaaac
    14941 agaaactagg aggtgcaaca tggatatagg tgaaaagata aaaagattaa gaacagaaaa
    15001 acaactgacc caggaagagt tagctaatag atgtgaattg tcaaagggat ttatttctca
    15061 attagaaaat aacttaacat caccatccat agctaccctt atagatatac ttgaaatact
    15121 aggtacaaat ttaagggaat tttttaatga aatagatgat gaaagaatca gttttacaaa
    15181 agaagatatg tttgaaactg aagatgaaga cttaaaatat aaattaaagt ggttaattcc
    15241 aaattcacaa aaaaatgaaa tggagccaat aataataaca ttgtatccag gaggacagta
    15301 taaagaagaa aagccacacg aaggtgaaga atttggatat gtattagcag gttcaatata
    15361 cgtccatata ggcgaaaaga aaaataaagt aaaaaaagga gagagttttt actttagacc
    15421 gaaagcaaat cactatatat caaacgaagg caaaactaca gcaaaagtga tttgggtaag
    15481 tacgccacca tcattttaga atagaggtgt ttaataattg gtagaaaata taatagaatt
    15541 aagggatata tctaaacatt atgaggaact tactgtttta gataatttca atttagatat
    15601 aaagaaaaat gagtttttaa cattacttgg accaagtgga tgtggaaaaa ctacaacatt
    15661 aaaaataata gcagggtttg agtatgctga tgatggtaaa gttttatttg aaggtaagga
    15721 gataaataat ttgccaccat atgaaagaca agtaaacaca gtatttcaaa aatatgcttt
    15781 atttccacac atggatgttt atgaaaacgt agcttttgga ctaaaaatta aaaagactcc
    15841 aaaagatgta atagatgcta aagtaaaaga ggttttgaaa cttgttgcac tagaaggttt
    15901 tgaaagacga caagttgaat cattgagtgg aggacaacaa cagagaattg caatagcaag
    15961 agctctagtc aatgaaccaa aagtattgtt actagatgaa ccacttggag cacttgattt
    16021 aaaattaaga caagagatgc aaatagaatt aaagagaatc caaaaaaaat taggaataac
    16081 atttgtgttt gtaactcatg accaagaaga ggctcttact atgtcagata ctattgttgt
    16141 aatgtataag ggaaaaattc aacaaatggg aacaccacaa gatatataca atgaacctaa
    16201 aaattcattt gtagctaagt ttataggtga aagtaacata tttgatggaa ttatgataga
    16261 ggattataaa gttaatttct gtaataggga ttttgagtgt gttgataaag gattcgaaaa
    16321 aaacgaaaat atagaggtag taataagacc agaagatata aaaatggtaa agccagaaga
    16381 aggaatgtta aaggggaaag ttacatctac agtgtttaaa ggagtgcatt acgagataga
    16441 attgaatgaa aatggaagat tgtggatact tcataatact aaaaatgctg aggttgggac
    16501 tgaactaggc atggatatat atccagaaga tatccatata atgagaaaag agagtaacta
    16561 agatgaaaag aaaatctttt ttagcatatc catatgtagt ttggagtgcg atatttgtaa
    16621 taattccttt aatactagtg gtatttttta gttttacaaa agaatcaggt ggaggttatg
    16681 cttttacact tgagaactat aaagaagtaa tcgaccctat atatataaaa gtttttgggc
    16741 gttctattct tttagcagga ggagcgacat tgatatgtct tgtagttggt tatcctgtgg
    16801 catatataat ttcaaaggct cgtgtaagca gaagaggttc attaatatta ttgtttattc
    16861 ttcctatgtg gatgaacttt cttttaagaa catatgcatg ggttgctata ttaggaaaaa
    16921 atggactttt aaatacattt ttaggttggt ttggaataca accattagca attttgtata
    16981 ctaactttgc tatacttttg ggtatggtgt ataacttttt accatttatg gttcttccaa
    17041 tatatacagc actgtcaaag atggataatg atttgataaa cgctgctcat gacttgggtg
    17101 caaataatat gacagtgttt agaaaaatta tatttccttt gagtttgcca ggtgtaatgt
    17161 ctggaataac tatggtattt atgccggcag tcactacttt tgcaatatca agactgttag
    17221 gtggaggtaa aatcatgctg gttggagact tgattgagca acagtttaca gtcgttggag
    17281 attggaactt tggttcagct atatcaatat ttatgatgat agtaatatta atatctatgt
    17341 ctataatgtc aaaatttgga gatgaatctg acaaagaagg cggtgggtta ctattttaaa
    17401 attaaagaaa ataacttcta atatatattt agcattagta ttcttatttt tgtatgcacc
    17461 catatttgct ttggtactat tttcttttaa tgattcaaaa tcaatggcaa gatggaatgg
    17521 atttacatgg aaatggtatg gtcaattact tcaaaatgag agtataatga gtgctttata
    17581 ttatactatt gtaatagcta tattggcgtc tgtaatatct acaatagtag ggactataag
    17641 tgctatagga atacataaga tgagaggtaa gagtaagaaa ttaatactaa atgtaaacta
    17701 tcttcctatt ttgaatacag agatagttac agcagtagct cttatgagtc tatttgtatt
    17761 tgtaaaaatg gaatttggat ttacaacaat gttattagca catataatgt tttgtttgcc
    17821 atacgttata ctttcagttt tgccaaaaat gaagcaatta cctgataata tagaggatgc
    17881 agctatggat ttaggagcaa cacctatata cgctcttaga aaagtaattt taccacaaat
    17941 aaaaccaggt atagtttctg gatttttaat tgcgtttact atgtcaatag atgactttat
    18001 aataagtttc tttaatgctg gaaatggtgt aagcaatctt tctatagaaa tctatggaat
    18061 ggctagaaga ggtataaaac ctgaaataaa tgcattatca acaataatgt ttgcagttgt
    18121 tctagggtta cttttactgg ctaataaaaa agaatctata gtaaggggga ctaaataatg
    18181 attaagttta aaaaagcaat atcccttata gctgtaggta tgatgacagt tactttattt
    18241 acaggatgta ataaagcaac agattctaca aaagttttaa atgtatataa tgttggagat
    18301 tatatagatg agtctcttat agataagttt gaaaaagaaa caggaataga tgttcaatat
    18361 tcaacttatg atactaatga aatgatgtat caaaaggtaa aaagtggtag tacacactat
    18421 gatttagtat ttccttcaga ctatatggta gagaaaatga aaaatgaagg tcttttggaa
    18481 aaattagatt ttaagaatat acctaatatg aagtatttag ataagagttt tttaaatcct
    18541 gtatacgaca aaactaatga atatagtgtt gcatatatgt ggggaactct tggaatattg
    18601 tacaataaga aagaagttaa agaccctatt gatagctgga atatactttg gaatccaaag
    18661 tataagggaa atataatgat gtttgactct gttagagata ctataggaat aactcttaaa
    18721 aaattaggat atagcatgaa cagtgtaaat ccaaaggaaa taaatgaagc taaaaatctt
    18781 ttaatgaaac aaaaggattt agtattggct tatgtaaatg atgaaggcaa ggatagattg
    18841 cttggtggag aagtggcaat gggtatgctg tattctgggg atgcagttac actaatggaa
    18901 caaaatcctg accttgatta tgctatacct aaagagggaa ctaataaatg ggtagatgct
    18961 atgtgtgtac ctacaacagc acaaaataag aaagaagctg agttgtttat aaacttttta
    19021 ttggacccag agaatgcaaa agtaaatgct gagtacatag ggtattcaac acctaataca
    19081 ggagctctaa aattattaga cccggaaata actgagaatc ctgttgcata tccatctaaa
    19141 gaagtactta ataaatgtga aacttttgaa gatattggtg aaaatataaa attatatgat
    19201 aaggcttgga tagagttaaa atcaaaataa taataactat aaagaggttg tctcgaaatt
    19261 gagataagcc tcttttttat tgttgaaaac atattcgatt tcgtacaaaa aagagtgatt
    19321 tatgagttaa aaaatttact ttgagacagt atttttttat taagttatat tcatacacaa
    19381 tttaaatact ttgttaataa atgaaaactt atttgtataa tgactttaat aataattaga
    19441 aagaatgggc aacttaatag aataattgga ggtgtaaatt tataaaaaat gaatattgta
    19501 aataaaaaaa tagatttatt aatattggct ttgctcgttt ttatattttt aattgttgga
    19561 atagtaatac tatccattag gacagaaaat aaattgaatg tgtttataat gctagcaata
    19621 atttttttta taataatgtt aacttatttg tcaaactcaa ttgtgggatt aataacaagt
    19681 tcaataatta tatttatgta tacatcctat attttatata acaatattac ttacaatatg
    19741 gatgttgaat ttatatcata tatgtggata atagcaatac caatatcttc aattataatg
    19801 ggtaatctga acaaaagcat aaatgaattg caaaacacaa ataaaaaact atctgaacag
    19861 tataaggaat tagtgactat tgatagtgaa actggactta gaaatttaaa gattttttat
    19921 aatgatgtaa atatggagat aagtaaatct attagacata atactgattt ttctcttatg
    19981 atagtaaaat taccatacta tggaaaccta cagactatat ttggtgaaag caaaactaat
    20041 aaaatagtaa aatatattgg ttcaaatatt atagaatgta caaggaatga ggatataata
    20101 tatagtttac aaaaagatat gctagggata ctaatgccaa atacatcttt ggaaggttca
    20161 aaagttgtta aagacagaat aaagaaaagg ataaaagaac taaatttaga tttaaacaat
    20221 aggggaaaat atgtaaatat agatgtcaaa attgcatttt tacaatatag ggatagtttt
    20281 ggagatagta taaattttaa gaatatagtt gaagaggaac ttcaatatga tgtttaaaat
    20341 attcaataaa agtctgttaa agttagtagc tatattatca atagtactga taatatcaaa
    20401 ttttagtcta tcttcatatg catcagaaat tacttctaga acagataatg tattaatcgt
    20461 atatgattct aagaaagaaa cagattataa tagagatatt ttaaatatta tgaggactct
    20521 attgggaaga tttagttcgg atataaagct attaaaattg agtaactatg atggtgagat
    20581 aaataaaaat tattacagtc acatatttat tttaggaatc aatgaaaatt catatgataa
    20641 tgataataaa actaaaaacc tgattagttc tttaaattta tataaaggaa ctatatgttg
    20701 gctaggatat ggtatagaga atcttttgga acataaaaaa tacaatttag actatatagg
    20761 aaaaacaaat aatatagtta gtgtaaatta taggggtaaa tcatataatt tagaagaaca
    20821 ttatattttt aacattgtag aatcaaaaga tacaagtaat aaagtaatag gaagtattaa
    20881 tgatacctta aataagtatc catatataat aaatgataaa aacctttttt atgtatcgaa
    20941 attagattta gatggtgttt tattttatat attttgcgat tcacttaacg atatttttaa
    21001 tattaagaga tttgataaag gacgtatatt tgtaagaata gaagatgtac atgctttcag
    21061 agacccaaaa gaccttgtaa atatagctga ttatttaagt agtaaggata taccatttac
    21121 aattgcattg ataccagctt atgtaaatcc aaaaaatcat aaaataacaa ctttatcaga
    21181 aagccctgaa attgtcaaag caataaaata tatgcaagat aaaggtggaa ctgtaatatt
    21241 gcatggatat actcatcaat ataagaaaga agaagtttct ggagaaggat atgaattttg
    21301 ggatggtaaa aaagatgagc ctttgaaaga aaatatgaag atatttgtca aagatagagt
    21361 tttaagtggt cttagagtat gtatagagaa tggaatttat cctcttgcat ttgaagcgcc
    21421 acattatgca atggaatcag atggatataa agaactaaaa aaatattttt ctacttatat
    21481 ggggcaacat caaaataaca ataaaaaatt tagtacaaat acatatcctt atattattag
    21541 ggatacagaa gagtttaata tacttatacc tgaaaattta ggatatatag acccagaaga
    21601 taagtttact tttcaacata taaaagaaaa tttagataaa ttatctattg taagaggttt
    21661 tagtggaggt tttttcttcc attcatattt gaacatagag tatctaaaaa acacgataga
    21721 atatttagaa aaacagaata tagaatttat gaatttaaga gattttaata attgggtaaa
    21781 agttgatgaa atacaaataa gaaataatgg agatgagatt atagtcaatt atgataggga
    21841 tttagaagaa aatattaaaa gtgatattag atttaagtct atatctaata ttagtaaaat
    21901 tttaatattc atagtatcta taagtgtttt aatatttatg attatattta tatattttaa
    21961 gagaatagat aaaaagaaat ttttaaaata aatgatttta tattagaatt aatattttaa
    22021 aatattttag gagaaattta tgagagattt aaatgtatca gagtgtctgt ttgtatttag
    22081 tctgtgtagt atatggtctt tattgcttat aaatattatt ttagctatgg gtggatatat
    22141 attttacttt aaaaactttg ataaagaaat aaaagaaatt gatgaatatc ctatggtatc
    22201 aatacttgtt cctgctcata atgaagctaa agtgataggt agaactgtgg aatctttatt
    22261 attactcaat tatcctaaga gtaaaatgga acttattgtt ataaatgaca attcttctga
    22321 taatagtaaa gaaattttag aagatataaa gaataagtac aataactata atttcaccat
    22381 aataaataca gatagtttaa caggtggaaa aggaaaatct aatgcattaa atattggata
    22441 tacgatttca aaaggagatt ttatagctgt ttatgatgca gataacacac cagatagaaa
    22501 tgcacttaga tatttagtac aaactatagt aatgaatgat gaattaggtg ctgttattgg
    22561 gaaatttagg actcgaaata aaaataaaaa cctattaacc aaatttataa acatagaaac
    22621 attaagtttt caatggatgt cacaagctgg tagatggcaa ctttttaatc tatgtacaat
    22681 accaggaact aactttatat tgaggagaag tatcatagag gaaattggag gatgggatag
    22741 taaagcaatt gcagaagata cagaaataag ttttaggata tataaattgg gatacaagat
    22801 aaagcttgta ccacaatcta taacatggga acaagagcca gaaactgtta aggtatggat
    22861 aaaacaacgt actagatggg caaaagggaa tatatatgta ttaatgaaat acataaaaaa
    22921 tatatttaag caaggaagaa ataagattgt atttgatata gcttactttt tttctgtata
    22981 ttttctattt ttaacatctg tcattatatc cgatatttta tttgttttaa gtatatctaa
    23041 gctagtagaa atttctattc caattaactt ttttctaata tggatactat cttatctttt
    23101 atttattata gaagtgagta taagccttac tatagaaaaa ggtgaagcaa caattaaaaa
    23161 tatattcata gttgcaataa tgtattttac atactctcaa ctatggttgt ttgtcgctat
    23221 aaaaggaatg atagagtatc taaaagatat aatttttaaa agagaagtta aatggtataa
    23281 aacagaaaga ttttaattca tgtacaggag gaattaacat gaaaaaattt attatatcaa
    23341 ttatatcttt agtattgttt ttttcaaata tatcacttat agacaaagta aatgctgacg
    23401 agactaaagt taaaaattat aagtttgaaa gagatataac tattgatggt gttataggaa
    23461 gtaatagtac tttttttgaa gtaaataaaa attgggatat tgaagacgtt ttattacatc
    23521 ttaattttag taagagccaa atattaaatg gagatgtttc aagtttaact gttttgataa
    23581 ataatgtacc aataaaatct attaaattaa atgctaagac aaattataaa aatacactag
    23641 aagtattagt tccaaaagat tatataattc aaggctataa cgaaataaag gtaaaaacat
    23701 ataaaacaat atctgataaa atttgccaag atgattccaa tactgggaac tggatggtta
    23761 ttcacaaaga atcatatata tcaattagat ataaacaaaa aaagatggaa aattcaataa
    23821 atgaatatcc gtatccatat gcagaaattg aaaataatct taaattagat acaacaatag
    23881 tagtaccaga taatatgact agaggagagt ctacagctgt atttaatttg gcatcagaat
    23941 ttggaaaaat aataaaaaat gatgatttaa agttagatgt taaattatat tcagaaatga
    24001 aaaattggtc agatgataat attatatata ttggtaaacc tgaaaataca gcagaagaaa
    24061 tcttggacat tttaagtaca gaggaacaaa cactattatc ttcgaactgt ctaatcaagc
    24121 aagtagattc accatacaat aagaataaga aaatgatggt tgtaattggt agtaatgaag
    24181 aaaatttaat aaaggcatca aatttattaa tagaaaatag actttctaat caagttttat
    24241 catcatcagt acttgtaaac aaagagacta atataaaaat caatagagaa caaaaactaa
    24301 acttaggtca tttaacttta aaagacttag gatattctga ctttttgttg gaaggagcat
    24361 ttaatcaaca agctttgttt gatgtcaaaa taccgacagg taaggtacta gatgatggtt
    24421 caaagattgt cttgaattta agatactctg acaatttaca ttttgaaaaa tctttagtta
    24481 cagtgtctat aaatgatgtt cttgtgggaa gtaaaaaatt ggataggcaa cattctaata
    24541 atgataagtt agaattaaaa atacctaaag atatagataa taaaaattat tatcaagtca
    24601 aattggtatt taatttgaat ataaaaaatt cgaattgtgt tactagagag agtaataatc
    24661 cttgggcata tgtatcaaat agttcttatt tggcgttatc tacaaaagaa aatgaagctt
    24721 tatcttttga aagttatccg tatccatttg ttagaaatga tgagtttaat gatttaactg
    24781 tgataatgcc agattattca gattcacaag ccatgacatg gatgtctaaa ctaggtgtta
    24841 ctttgggtgc aaatataaat tcacataatg gaaatatgaa tgtaattaga ggaaaagagt
    24901 ttagcgacaa gtataaggac accaatatta tagtttttgg agtaccgcat aataattctg
    24961 ttataaagat gttaaataat aatttaaata taaaattcga taaaaattac tccaattttt
    25021 tatcaaatga taagttaagc tttattgatg attatgggaa aaatatttct accattcaat
    25081 taataaaatc accatataat aatcaaaaaa atatcatggt aattagttca atgaatgaaa
    25141 aaaacttata tttaggaatg gattatttgt tgaataaaag taaagttaat gatttaaaag
    25201 gagatacatt gataattgat gaatatggag aggtagaaga tttagcttat aatttaaaac
    25261 ctaagaaaga agtaaaaaat tcaagttgga atatgagtat aaataagaca accaaagtat
    25321 ttttaatgat ttcttttatt actataattg tagtcataat attatcaatg ttatatataa
    25381 aaaaatataa aagaaggtaa tattataaat t
//
LOCUS       DAFNNB010000041        24707 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0041, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000041 DAFNNB010000000
VERSION     DAFNNB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0041"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            104..1303
                     /locus_tag="L3M60_001478"
     CDS             104..1303
                     /locus_tag="L3M60_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBN6226156.1"
                     /translation="MKSAYKGYEAYSYLEKGKDISSIEMCKGDKRVKEYLIELDDIQE
                     SRVKSIVDEKIFISLHEHPVLFPEYLERDIFEYNREGKQRCAYEALSRGYYDAIFDNL
                     MDGVCTITSNSGWKWDDILIDLGMRLCDLAHQDFVIKCEKVEDIYRAKSEGKVALIPT
                     LEGAAPIENELDRIDILYGFGIRLMGITYSESNALGSGLKEEKDGGLTNFGKKAVERM
                     NKIGMAIDCSHVGVQTTLDVIEYSKDPIILSHVGARSLWNTKRLTPDEVLIACAKKGG
                     VIGVEAAPHTTITEKNKEHSIESFMEHFEYIKNLVGIDHVAFGPDTVYGDHVKLHSVL
                     ATKFDIKKITGTKEHPKVEYVKGLENPTETSKNIVRYLVKHGYSDEEIEKVLGRNILR
                     VLKQVWK"
     gene            1355..2050
                     /locus_tag="L3M60_001479"
     CDS             1355..2050
                     /locus_tag="L3M60_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5058 family protein"
                     /protein_id="HBN6226157.1"
                     /translation="MDYLKLANHPLLWIASTIAVSIVVVQSLIFAIKSCKVGKTMGIT
                     DKQIKSAVKSSAISAIGPSMTIFAGMVSLIVTMGGPIAWMRLSFIGSVIFESMSAGFG
                     TDALGITLGSPEMTKLAFTNAVWTMILGSLGWIIFTLLFGHKLDKITNVISSGKKSFI
                     PIISLGAMLGSFAYLNADRVLRFDNGTIACISGMAIMVILCILEKKKNIKWLREWGLT
                     ISMFSGMIIASVI"
     gene            2063..2740
                     /locus_tag="L3M60_001480"
     CDS             2063..2740
                     /locus_tag="L3M60_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226158.1"
                     /translation="MSKVKSIYNEEYLPFMIRYGRLTLSLGIIAALVPGIILSFGFGI
                     MPPISALLASTMAIVSMSAPNYIIEPVSYSPILGIPGTYMSFLSGNISNMRLPCSIAA
                     QKAAEVESGTEEGSIISTIGIAVSILVNIIILTIGVILGGSVLSKIPAEVVEKLNLIL
                     PALFGSVFGQVFLQDKKLGLVAIVISVLTIILSKQGIIPQSLVVLICVFGTILIARAM
                     YKDKLSD"
     gene            2905..4506
                     /locus_tag="L3M60_001481"
     CDS             2905..4506
                     /locus_tag="L3M60_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBN6226159.1"
                     /translation="MMYDLILKNGFIIDGTGNPGFYGDIAIKDNLIAKIDYKINGNTN
                     KEIDCCGKVITPGFIDPHVHEEIVAILDGKFEKFLKQGVTTTINGNCGHSITPYSSEN
                     VYEYMYKNGLLLEEEKKYLIDKNKYWNNFTEYCDLISKSGISINMGFLLGHGTIRWSV
                     MGGSKDRHPTEEEKNEITNIINDGMKSGAFGISTGLAYIPSKYADIDELVDIAKQIKE
                     YDGIYTSHIRDYIGRYNAVKEAIEVGQKSGARVQVSHLSPVEIEAFDEILKARYNGVD
                     IMVDTVPRSSGHCMKKKRVIQFIMAISSSLFELGIDGVMDALKNEEGRALILKEAFIL
                     GDRGSIILLNTKDIDMEKKSIREIAIQKDIDEDKLLLDLLLDGDEELIFCLGGMYRSD
                     FPDKLHDNKIIDNPFVMVGSDCLFSVGGDMSWFELQRRGAFSIFFNMYRKSGVRLEEI
                     VRRVTSLPARQFKIKNRGILKEGLIADIAVIEIENYSYPRSEDIDFSKPQSLAKGVEY
                     VIVNGKIALEDGNITENKCGEVLRR"
     gene            4972..6261
                     /locus_tag="L3M60_001482"
     CDS             4972..6261
                     /locus_tag="L3M60_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBN6226160.1"
                     /translation="MRELLNVTLIIILVLINAFFVACEFAMVKLRSSRIDTMITEGNN
                     NAKRCKIIKDNLNSYLSACQLGITLCSLALGWMGESTVKELILPIISIFNLSESIVYT
                     ISISISFLIITMMEVVIGELVPKALALYNTEKIMLSTSFLVVGFYKLTYPIIYFFNLS
                     TDLFLKPFGYSQADEVDEPHTGDEIRILVEESYKSGLIDESEQRLVDNIFEFEEKKIR
                     EIMVPRTDMVCIYESDSEEKILAILKEEGVTRYPVCRKNKDDILGFVHIRDLYNQKIN
                     ENKIELEEILRDIIYISENLTIDKALERIRKEKLQLAIVVDEYGGTSGVVTIEDILEE
                     IVGEIQDEFDKEETHIKKIGAGSYLVDGTESINYINKYFGLDIEHDGFDSIGGWISYM
                     LGSNIKVNQSVTFENYNFTILELDKLRVVKLIIKSVI"
     gene            6290..6970
                     /locus_tag="L3M60_001483"
     CDS             6290..6970
                     /locus_tag="L3M60_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HBN6226161.1"
                     /translation="MQKVEGIIFDMDGVLFDSERISLEFWIETFEKYGYTMTKEIYTS
                     VMGRNRKGIIEGLTNIYDSSVPIIDLYDEKTKNMIEFMDREGAPIKLGVNELISFLKE
                     NGYKMAVATSTKRERAVKRLAKANLKDYFDAIVCGDDVVNSKPNPEIFLKAAKKINVN
                     PQNCIVIEDSPMGVEAAYNGGIRCINVPDLKEPDEQIKSQSHKILENLLEVREYLKSL
                     SSKECHNL"
     gene            7071..7610
                     /locus_tag="L3M60_001484"
     CDS             7071..7610
                     /locus_tag="L3M60_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBN6226162.1"
                     /translation="MDNVGSLLYFLFNFFLYGFIGWIIENLYSYYKEGHFQEDGFLNG
                     PFKPMYAIAMSIIIEVSRFIDPNNIFTLLLLCLVVPTVVEYITGILMKVYFNKVYWDY
                     SDYKYNSRGIVCLKFSIYWTILTFIGVRYFQVYIVDSLYSLIIPYWPILVIILLATLF
                     VDEVLTIKFLRGNKDIVER"
     gene            7786..8805
                     /locus_tag="L3M60_001485"
     CDS             7786..8805
                     /locus_tag="L3M60_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBN6226163.1"
                     /translation="MMGKVRFGIVGTSNIANIFLKAASKVKDFELVAIYSRNLEKARE
                     FGMSHGASIFFDDLEQMAKSKDIDAVYIASPNALHSRQAIICLKHKKHVLCEKSLASN
                     LKEVKLMIKTAKDNNVLLMEAMRITCVPNFRAVKENLYKIGKIRRFFGSYCQYSSRYD
                     KYKNGIIENAFKKELSNGALMDIGVYCIHPMVNLFGAPKSVKAVSHVLQTGVDGEGSA
                     VFQYDDMDAIIQYSKVADSYIPSEIQGEEGSIIIEKLNLFEKAIIKYRDGREEDISIP
                     KEKAEEKPREIEGMYYELVEFISLINNNKIESNINSYENSIIVMEIMDEIRRQSSIVY
                     PADSI"
     gene            9095..10285
                     /gene="dltD"
                     /locus_tag="L3M60_001486"
     CDS             9095..10285
                     /gene="dltD"
                     /locus_tag="L3M60_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HBN6226164.1"
                     /translation="MRKLIYFITPFIIGVVFLFGLDKFLDSKTDELLREKNLLPIMDD
                     TLSDIKDKGVTANNHFLREKDIMILGSSELSNSTKQHPKYYFNTNRSKNKVFAIGRAY
                     TQTLQDAAILGSMNPNIDNKKVVLLISMQWFMEKDGVTSHHYQSRFSPIQFYRFLDNP
                     KISKQNKIEYAKKSSKLLWGSDEYKAEALYAKLYEPKTLLEKAEKVLLEPYFQGRKYC
                     IALKEKGILYKRLIKLDKKRATKRKSPINWSHERKKAIEDAKKRVGKNPLNIDNYYYK
                     QHFKDGIDQYKGRDKDVNLLTSKEFESYKLMLNVCTDLGIKPVVVLIPSMDKFYNLTG
                     ISEKERNQYYDKAQNIAESKGFEVLNLKDKGSDKYYLRDVMHLGTKGWVDVCERLFKI
                     FKEQ"
     gene            10406..11920
                     /locus_tag="L3M60_001487"
     CDS             10406..11920
                     /locus_tag="L3M60_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase"
                     /protein_id="HBN6226165.1"
                     /translation="MKIIEGIKKYSNTDRIALICNGDKLSYKDLNEYSDAISIFLKDV
                     YKEEDTPIVIYGNKENMIMACMIGALKSGRAYVPLDISFPIDRVFEVTKEIKPKVLFN
                     FSDEKDFGDINVIDMDKLKDIINEYQGKSLDKENWVKDDENAYILFTSGSTGKPKGVQ
                     ISSNNLDSFSDWISPYLNIDGSEKVIMNQPAYSFDLSVTTIYPGLIHGATLFSISKDV
                     LADYKELFRQFSISDIAVWVSTPSFAGVCITEKEFNSKMLPKLESMIFIGEALSKNLT
                     KELMSRFPNTRVINGYGPTEATVGVSVNDMTQKAIEDEKSLPVGYPMSNCKIKILDEA
                     GNELKENEKGEIIIIGPSVSKGYFNNKEKTDEVFFYDEIDGIKWRAYKTGDMGYLLDG
                     NIYYCGRKDFQIKLNGFRIEIEDIENNLRKVHNVKNVVVLPVYKDEKIAYLKGIVELN
                     EKNDLSNIKNGMIIKKELGKYIPSYMIPRNISIISEFPTNINGKIDRKKLMEEI"
     gene            11920..13068
                     /gene="dltB"
                     /locus_tag="L3M60_001488"
     CDS             11920..13068
                     /gene="dltB"
                     /locus_tag="L3M60_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HBN6226166.1"
                     /translation="MIFSQYGDYFYLYILLLTSIPAVILGLMGKNIKYYGMLASLFMI
                     FLIVGIDVQLKYLVIFIILEVIIVKGYEYVRRKTKNKYVYWSFLFISMLPIIINKISP
                     VTSFGMIGFIGISYLNFRAIQMVIEIYDGAIKEVKISKMLYFMLFFPTLSSGPIDRSR
                     RFEQDLEKQISRKEYIEEYLLPGIKNIVMGVGYKFVIAFLINTYWVSIIPKDMSFINI
                     LSYMYAYSLYLFFDFAGYSLFAIGTGYIFGIQVPINFDKPFISKDMKEFWTRWHISLS
                     RWFGDYIFSRFVMSSMRKKRFKKRTTAAHVAQMITMITMGFWHGLTWYYVAYGVYQGL
                     ALVLTDIYQRKSKFYKKHKKEKWFERVQIFITFHIVCFGLLIFSGYLA"
     gene            13087..13317
                     /gene="dltC"
                     /locus_tag="L3M60_001489"
     CDS             13087..13317
                     /gene="dltC"
                     /locus_tag="L3M60_001489"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HBN6226167.1"
                     /translation="MQETVIEIFEDVLGTDEIREDLDLNLFETELLDSLAIIEVLLEI
                     ENRLGIELQPTDLERKDMSTVNNLVKFLETRK"
     gene            13532..14317
                     /locus_tag="L3M60_001490"
     CDS             13532..14317
                     /locus_tag="L3M60_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN6226168.1"
                     /translation="MSLNSIDRKKEILKLLDSKGKVNTKELVKRLEVSSETIRRYLEE
                     LENENKLKKVYGGAIKINYGVIEPEHIKRNSTNLDKKQSIAKYASKFVNDNESIIIDE
                     GTTNLQIVDYIISVNNLKIITNSYPVLSKLISYENQGLFDGEVIFIGGKVNLKHQRTA
                     CPMSIDTMKNLYVDKAFISSEGILDSFGISSIDQNKALLSKEYIKNSKESFVLCDSSK
                     IGIASLYKIADLEELDFVICDVEPPNEWKDKLNEINITWIEAK"
     gene            14607..16517
                     /gene="phnW"
                     /locus_tag="L3M60_001491"
     CDS             14607..16517
                     /gene="phnW"
                     /locus_tag="L3M60_001491"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HBN6226169.1"
                     /translation="MKKIYGEKIKAVVFDWAGTTVDYGCFAPLNVFIEIFKRRGIDVT
                     MEEARKPMGKLKIDHIREMCEMDRIKNLWSDKFGKVPIEDDVNELYAEFEPMLFETLE
                     EYTTPIPHVIETIEKLRENGLKIGSTTGYTREMMDIVQPNAAKKGYSPDFLVTPSEVS
                     QGRPYPWMCYKNAEALGVSPMSSMVKVGDTISDVKEGVNAGMWSVAVIKGSSELGLTE
                     EEVENMDKEELKIKMNTVSKKFKEAGAHFVIETMAELEDVLVKIENKTIKSDFVPEND
                     YILLTPGPLSTTKSVRASMLKDWCTWDVEYNDLVQDVRRRLISLGTKNTDKYTSVLMQ
                     GSGTFSVEATIGSTIPENGKLLVIANGAYGKRMKDICNYLNIEFVDCTFKDVEAVDLN
                     VVENLLKENKDITHISMVHCETTTGRLNPIQEVGKLAKEYNKVYIVDAMSSFGGIEID
                     VEDFNIDFLVSSSNKCIQGVPGFGFIIANKEELSKCKGIAKSLSLDVYAQWETMEKNN
                     GKWRFTSPTHVVRAFYQALLELEEEGSVEKRYARYKENQFTIASRLKSLGFDTLVNDN
                     AQSPVITTFLYPKNAKFEFMEFYTYLKANGFVIYPGKLTDIDTFRIGSIGEVYPTDME
                     RLADVIERFINR"
     gene            16806..17627
                     /locus_tag="L3M60_001492"
     CDS             16806..17627
                     /locus_tag="L3M60_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226170.1"
                     /translation="MNEELSSLRFDGVGKCKGGIFDNVNIAGTGKIEGSVKCSKFNSS
                     GSAKVFGTTECNEFFTSGLSKVEGNILASKVEISGLLRCTGDINSVEIDTSGIINVEG
                     SIKSDIIRGEGYLKANENIDCENINLSGIMECNGFLNCEEVNISLNGISNFNEVGASS
                     INIKKGHGDYCKKFRMLEKKHINFEKVSSNLISLGLLSIRKYIESKLFANIIEADEIS
                     LENSEVKVVRGKNVKIGKGCYVESLEYSEIIEIDEDSIVGEIKNINEKVKLKKDK"
     gene            17668..18435
                     /locus_tag="L3M60_001493"
     CDS             17668..18435
                     /locus_tag="L3M60_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HBN6226171.1"
                     /translation="MEKGSIKILGEGFISSGEYESIRIVGSAKSKGNIKSDEVKVLGD
                     AKFEGDINIKNFVVNGKTIIRGNLKAERVRVNGQLSIYGDAEIDDLVITAQQKVDGKL
                     KCNNVTIRGQLSVSENMYAQDIKIYGEMRNKSNIECEDIKVYGVIECDGLLNGENIYV
                     YSGGGSYCKEIGATNIKIGRSKDVFSLIKIINIFSGKFKCNLIEGDTIELEDASIKDI
                     RGKSIFLVDNCDVGNIEYSDNLSVSDNSTVKNSIKVG"
     gene            18443..19063
                     /locus_tag="L3M60_001494"
     CDS             18443..19063
                     /locus_tag="L3M60_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbD family protein"
                     /protein_id="HBN6226172.1"
                     /translation="MEELISKKDLLSKTNISYGQLYRWKRKNIIPEEWFIKKSAFTGQ
                     ETFFPRDKILERIELILSMKEETSLDDIANMFMKKDINKEFDIDFILSKEVICNYTKE
                     IFQNLYKKEQSIGKKELLILSIIEKFLVKSTITFEELKLIVNIIEENFTSIYNENGKI
                     YLFRKFGVPFVVGCLDYKKVKFEKDVVKIIEIDLTKEINDISIKLS"
     gene            complement(19365..19952)
                     /locus_tag="L3M60_001495"
     CDS             complement(19365..19952)
                     /locus_tag="L3M60_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBN6226173.1"
                     /translation="MNLKDSKTKENLLRAFAGESQARNRYNISASVAKKEGLYIIESL
                     FNYTADQEKSHAKQFFSKLKEFAGENINISGAYPVDLYDKTAQHLKAAQHNELQEWDD
                     VYKNFGKTAREEGFESIAKLFENIAEIEKVHGDRFGRYATDLEEGTLFRRNEDIQWIC
                     THCGHIHVGKEAPKACPVCLHPQGYFLDFKNSLFE"
     gene            complement(20038..20475)
                     /locus_tag="L3M60_001496"
     CDS             complement(20038..20475)
                     /locus_tag="L3M60_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6226174.1"
                     /translation="MEDKYIVHFISKTKTNMIKFIENKLSKNDLDELIPTHGNILTAL
                     YENNGILTMKEIATKIGKDKSTVTVLVNKLINLGYLERQKCTNDKRITYIKLTKKALL
                     IEDTFNSISTQVKETAYHNITENEKQEFLRILKKINDNFKNAD"
     gene            20579..21577
                     /locus_tag="L3M60_001497"
     CDS             20579..21577
                     /locus_tag="L3M60_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdH/YhfP family quinone oxidoreductase"
                     /protein_id="HBN6226175.1"
                     /translation="MNKKFKSLYITEEEGNFKRNIVERDVDTLPEGEVLIKVKYSSLN
                     YKDALSANGNKGVTRKYPHTPGIDASGIVESSSCDDFKIGEEVLVTGYDLGMNTSGGF
                     SEYIRVPSSWVVRLPKNLSLKEAMDYGTAGFTAALSVYKFIDSVDKDMGDVLVTGGTG
                     GVAVIATKILIKLGYSVVVSTGKLEEQKQALLNLGVKDVIHRSEVDDNSGRPLLRPRW
                     AGVIDTVGGNTLATAIKTTNYCGAVTTCGNAGGVDFTSSVYPFILKGITLYGIDSVDC
                     PMNLRLKIWDLLSNEWKLDDLDDMVNVVSLEELNDSIDSIMAGTHVGRTVVDLEKI"
     gene            complement(21698..22624)
                     /locus_tag="L3M60_001498"
     CDS             complement(21698..22624)
                     /locus_tag="L3M60_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBN6226176.1"
                     /translation="MNKENKNVRIAVVQASPVIMDLEKTVEKALGLIKEAGRKGANIV
                     VFPEAFIPAYPRGLSFGFVVGSRTMKGREDWKRYYDNSVPVPSATTDLLGKAAQEAGV
                     YLSIGITERDGNDINCTLYCTNLFFSPEGKLIGKHRKLKPTGTERCIWGEGDGSTLTV
                     VDTPYGKMGSLICWENYMPLARTALYAKGVKLYIAPTADSREEWQATMKHIALEGRCF
                     VIGCNQYVEKNMYPTDLNYYKELDAEPEIMCPGGSCIVDPFGKYVAGPIFNKEEMLIA
                     DLDLEKIVLSRLDFDSEGHYSRPDVFELIVHE"
     gene            23010..24611
                     /locus_tag="L3M60_001499"
     CDS             23010..24611
                     /locus_tag="L3M60_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226177.1"
                     /translation="MKEKIYYNGNIITMEDSICGDAILIKDKIIKKIGTKEEVFALKN
                     KDTEIIDLQGKTLMPSFIDSHSHLIAFATTLKLVPLEDATSFKDIVKKIQDFKESNNI
                     KKGDWIIGFSYDNNFLEENKHPDKNVLDSASSENPILISHASGHMGVANTLGLKQLGV
                     TNETYDPEGGHIGRVEGSEEPNGYLEENAFFNVSSKIKPPSSNEIFNSIEKAQDIYLS
                     YGITTAQEGLMEENQFNLLKAMANQNKLKIDVVGYVNLKKSKPVADNNREFIKKYINR
                     FKIGGYKIFLDGSPQGKTAWLSKPYENSDDGYCGYPIYKDEEVEKFIDISLKEKMQLL
                     THCNGDAAAEQLIDVFEKVLTLKEQSSNNMRPVMIHAQTVREDQIDDMKRINMIPSYF
                     VAHTYYWGDIHIKNLGEDRAFKISPLKTTIEKGLIYTLHQDTPVIAPNMLETVWCAVN
                     RITKKGVQIGENEKISPLDALKGVTINAAYQYFEEDKKGSIKEGKLANLIILDENPLT
                     IDPMKIKDIKVLQTIREGEVLYSLK"
BASE COUNT         9697 a         2539 c         4413 g         8058 t
ORIGIN      
        1 ttattttttg aaagatttat tctacaatta aaatttatat tttggcatat caattgctat
       61 atagaaaaaa tataaaaaat tgtagaactt ggaggatatt gttatgaagt cagcttataa
      121 aggatatgaa gcctatagtt atttagaaaa aggaaaagat atttcatcta ttgagatgtg
      181 taaaggagat aaaagagtta aagaatacct tattgaatta gatgatattc aagaatctag
      241 ggttaaaagt atagttgatg aaaaaatatt tatatcatta catgaacatc cagtgttatt
      301 tccagaatac ctagaaagag atatatttga atacaataga gaaggtaaac aaagatgtgc
      361 ttatgaagca ttatcaagag gatattatga tgctatattt gataatttaa tggatggtgt
      421 atgtacgata acatcaaatt caggatggaa atgggatgat atattaatag atttgggtat
      481 gagactatgt gatttagctc atcaagattt tgtcataaag tgtgaaaaag tagaagatat
      541 atacagagct aaaagtgaag gtaaagtggc attgatacct acactagaag gtgcagcacc
      601 aatagaaaat gaacttgata gaatagatat actatatgga tttggtataa ggttaatggg
      661 tataacatac agcgaatcca atgcattagg ttcaggacta aaggaagaaa aagatggagg
      721 tcttacaaat ttcgggaaaa aagctgttga aagaatgaat aaaattggaa tggcaataga
      781 ttgctctcac gttggagttc aaactacact tgatgttata gaatatagta aggacccaat
      841 aatattaagt catgttgggg caagaagttt atggaataca aagagactaa ctccagatga
      901 agtattaata gcatgtgcta aaaaaggagg ggttataggc gtagaagcag cacctcacac
      961 tacaataaca gaaaagaata aagaacattc aatagagtca tttatggaac attttgagta
     1021 tattaaaaat ttagtaggaa tagaccatgt agcttttgga ccagatacag tatatggtga
     1081 ccatgtaaaa ttacactcag tactagctac taaatttgat ataaaaaaaa taacaggtac
     1141 aaaagaacat ccaaaagtag aatatgtaaa aggtcttgag aacccgacag aaacttcaaa
     1201 aaacattgtc agatacttag taaaacatgg atatagtgat gaggaaatag aaaaagtact
     1261 aggtagaaat atacttagag tattaaagca ggtttggaaa tagatatatt agaaatatta
     1321 acaaaatctg aggatttata gggaggtttt ataaatggat tatttaaaat tagctaatca
     1381 cccacttttg tggatagcga gtacaatagc cgtatcaata gtagtggtac aatcccttat
     1441 atttgctata aaatcttgca aagttggtaa aacaatggga ataacagata aacaaataaa
     1501 gtcagctgtt aagagtagtg ctatttcagc tattggacct tcaatgacta tatttgctgg
     1561 aatggtatca cttatagtaa ctatgggtgg tcctatagca tggatgaggt tatcgtttat
     1621 aggttctgta atttttgaat caatgtcagc aggatttgga acagatgcat taggtataac
     1681 tttaggttct cctgaaatga caaaacttgc atttacaaat gcagtttgga ctatgatatt
     1741 aggttcttta ggatggataa tatttacatt actatttgga cataagctag ataagataac
     1801 caatgtcata tctagtggaa agaaatcatt tataccaata atatctctag gggcaatgtt
     1861 gggttcattt gcatatctaa atgctgatag ggtgttgcgt tttgataatg gaacaattgc
     1921 atgtataagt ggaatggcta taatggtaat attgtgtata cttgaaaaga agaaaaatat
     1981 aaaatggcta agagagtggg ggcttacaat atcaatgttt agtggaatga taatagcctc
     2041 agtaatataa ggggggagtt ttatgagcaa agtaaaatct atatacaatg aagaatattt
     2101 accttttatg ataagatatg gaagattgac actatctttg ggtattatag cagcattagt
     2161 tcctggaata attttgagct ttgggtttgg tattatgcct ccaatatcag cattattagc
     2221 aagtacaatg gcaattgtca gtatgagtgc tccaaactac ataatagaac cagtttctta
     2281 ttcaccaatc ttaggtatac caggaactta tatgagtttt ctttctggga atatatctaa
     2341 tatgaggcta ccatgttcaa tagcagctca aaaggcagca gaagttgaat caggtacaga
     2401 agaaggctcg ataatatcta caataggaat agctgtatct attttagtga atataattat
     2461 actaactata ggggtaattt taggaggaag tgtattatct aaaattccag ctgaagtagt
     2521 agagaaatta aacttaattt taccagctct atttgggtca gtttttggac aagtattttt
     2581 acaagataaa aaactaggtc tagtagcaat tgtaatatct gtattgacta ttatattatc
     2641 aaagcaaggt ataataccac agtctttagt agttcttata tgtgtatttg gtactatatt
     2701 aattgcaaga gctatgtata aagataagct tagtgactaa ttacaatgaa ataataatga
     2761 attttcaaat ataaaataca atgatttgat tacgcaaaat tatatgaagc aggaactctg
     2821 tgactttagt catgggaggt tcagtttaaa atataaaaat tacattaaga ctatttaaaa
     2881 actggttttt aaggagggaa tattttgatg tatgatttaa tcttaaaaaa tggatttata
     2941 atagatggta caggtaatcc tggtttttat ggagatatag caattaaaga caacttaata
     3001 gcaaaaatag attataagat aaatggcaac acaaataaag agattgattg ctgtggaaaa
     3061 gttataacac ctggatttat tgaccctcat gttcatgaag aaatagtagc aatactagat
     3121 ggtaaatttg aaaagtttct aaaacaagga gttacaacaa ctataaatgg taattgtgga
     3181 cattctataa caccatattc ttctgaaaat gtatatgaat atatgtataa aaatggtcta
     3241 ttacttgaag aagaaaaaaa gtatcttata gataaaaata aatattggaa taattttaca
     3301 gaatattgtg accttatatc taaaagtggt atttctatca atatgggatt tttgttggga
     3361 catgggacta taagatggag tgttatggga ggttctaagg ataggcatcc tacagaagag
     3421 gaaaaaaatg aaattacaaa tataataaat gatggcatga aaagtggtgc ttttggaata
     3481 tctacaggat tggcatatat accaagtaag tatgctgata ttgatgaact tgttgatatt
     3541 gcaaagcaga ttaaagaata tgatggtata tatacatctc atatcagaga ttacatagga
     3601 agatataacg cagtaaaaga agctatagag gttgggcaaa aaagtggtgc tagggttcaa
     3661 gtatctcact taagcccagt tgaaatagaa gcatttgatg agatattaaa ggctaggtat
     3721 aatggtgttg atataatggt tgatacagtt ccaagaagtt ctgggcattg tatgaagaaa
     3781 aaaagagtaa ttcaatttat tatggcaata tcatcaagtt tatttgaatt aggtatagac
     3841 ggggtaatgg atgctcttaa aaatgaagag ggtagggcac ttatattaaa agaagcattt
     3901 atacttggag atagggggag tataatactt ctaaacacta aggatattga tatggaaaag
     3961 aaatcaatac gtgaaatagc catacaaaaa gatatagatg aggacaaatt attactagat
     4021 ttacttttag atggagatga agagcttatc ttttgtttag gaggaatgta tagatctgat
     4081 ttcccagata aactacatga taataagata attgataatc catttgttat ggttggttca
     4141 gattgtttat ttagtgtggg tggggatatg tcatggtttg aactacaaag gagaggagct
     4201 ttttcaattt tcttcaatat gtacagaaag tctggcgtta gattagaaga gattgtaaga
     4261 agagttactt cattaccagc taggcagttt aaaataaaaa atagaggtat tcttaaagaa
     4321 gggttgatag ctgatatagc agttattgag atagagaact atagttatcc taggtcagaa
     4381 gatattgatt tttcaaagcc acaatcactg gctaaaggtg ttgaatatgt aattgtaaat
     4441 ggaaaaattg cattagaaga tggaaatatt actgagaata aatgtggtga ggtattaaga
     4501 agataaggtt ttaaaaataa tagggattta gaaagcaaat aaatgtctaa gtaagtaaag
     4561 acgaagtttg agatttattt aaataagaat catgcttcgt ttttacttat agtattaaga
     4621 tttattttaa tagtaagata atatgtatac aaaaaatatt tatattttat agtgaaaaaa
     4681 ttaatatttt taatcattaa attatgtaaa aaaaatggat gcatagataa ctaaatcatt
     4741 taagaaaaga ttttttagaa ataaaaaatg ataactaaag aaccccttta atatttatca
     4801 aatagacaat cataaatatt aaaggggttc tttagtttat aaaaattatt tgtaataaag
     4861 taggatatat taataaatat gatagaatat atattataaa ttaaatttat aaatataagg
     4921 ggggaacttt gagttcttat agatttctta cactataaga accaatactt tatgcgggag
     4981 ttattaaatg taacacttat aataatactt gttttgataa atgctttttt tgtagcttgt
     5041 gaatttgcta tggtaaaact tcgtagttct aggattgaca ccatgataac tgaaggtaat
     5101 aataatgcaa aaagatgtaa aataataaaa gacaacttaa attcctatct ttctgcatgt
     5161 caacttggta taacactttg ttcacttgca ttaggttgga tgggggaatc tacagttaaa
     5221 gagttgattt taccaataat aagcatattt aatcttagtg aaagtattgt atatactata
     5281 tcaatttcca tttctttttt aattataaca atgatggagg ttgtaatagg agaattggtt
     5341 cctaaagctt tagcacttta taatacagaa aaaataatgt tgagtacatc ttttttagta
     5401 gtaggatttt ataagttaac atatccaata atatattttt tcaacttaag tactgattta
     5461 tttcttaaac catttggata ttcacaagct gatgaagttg atgaaccaca tacaggtgat
     5521 gaaataagaa tactagtgga agaaagttat aaaagtgggc ttatagatga atcagagcaa
     5581 agattagttg ataatatttt tgaatttgag gaaaagaaaa ttagagagat aatggttcct
     5641 agaaccgata tggtatgtat atatgaaagt gactctgaag aaaagatatt agctatttta
     5701 aaagaagaag gtgttacaag atatccagta tgtagaaaaa acaaagatga tatattaggt
     5761 tttgtacata taagagattt atacaatcaa aagataaatg aaaataaaat tgaactagaa
     5821 gagatattga gagatattat atatatttct gaaaatttga ctatagataa agctcttgag
     5881 aggataagaa aggaaaaact tcaacttgct atagttgtgg acgaatatgg tggtacatct
     5941 ggtgtggtaa cgatagaaga tatactagaa gagatagttg gagaaattca agatgaattt
     6001 gacaaagaag agactcatat taagaaaata ggagcaggtt cttacttagt tgatggaact
     6061 gaatccatta actatattaa taagtatttt ggacttgaca tagagcatga tggatttgac
     6121 agtataggtg ggtggatatc atatatgctt ggttcaaata ttaaagtaaa tcaaagtgta
     6181 acttttgaaa attacaattt cacaatatta gagttggata aattgagggt tgtaaaatta
     6241 atcataaaaa gtgtaatata attatatcaa aaaatattgg aggaattgta tgcaaaaagt
     6301 agaaggaatt atttttgaca tggatggagt tttatttgac tcagagagaa tttctcttga
     6361 attttggata gagacgtttg aaaagtatgg atatactatg actaaggaaa tctatacatc
     6421 agttatggga agaaatcgta aaggtataat agaaggtctc acaaatatat acgatagttc
     6481 tgtaccaata atagatttgt atgatgaaaa aactaaaaat atgattgaat ttatggatag
     6541 ggagggagct cctatcaaat taggagtcaa tgaactaatc tcttttttaa aggaaaatgg
     6601 atacaaaatg gctgttgcaa catcaacaaa gagagaaaga gcagtaaaga gattagctaa
     6661 ggcaaattta aaagattact ttgatgctat agtttgtggg gatgatgttg taaactctaa
     6721 accaaatcct gagatattct taaaagcagc taaaaagata aatgttaatc cccaaaattg
     6781 tattgtgata gaggattcgc ctatgggagt tgaagctgca tacaatggtg gtataagatg
     6841 tataaatgta ccagatttaa aagagccaga tgagcaaatt aaatcacaat cacataagat
     6901 acttgaaaat ttattagaag ttagagaata cttaaaaagt ttgagctcta aagaatgtca
     6961 caatctataa tataaaatat taattaaata aattaaatat aatttctttg atttagaata
     7021 aatccatgaa tatgagaaaa aataagtata aacagctagg aggaatattc atggataatg
     7081 taggttctct attatatttt ttatttaatt ttttcttgta tggttttata ggttggataa
     7141 ttgaaaattt atatagttat tataaagaag gacattttca agaagatgga tttttaaatg
     7201 gtccatttaa accaatgtat gcaattgcaa tgtcgattat aatagaggta tcaagattta
     7261 ttgacccaaa taatatattt acattgcttt tattatgtct agtagtgcca acagtagtag
     7321 aatatattac aggaatactt atgaaagtat attttaataa ggtctattgg gattattcag
     7381 attataagta caatagtaga ggaatagtat gtctaaagtt ctctatatat tggactatat
     7441 tgacctttat tggagttagg tattttcaag tatatatagt tgatagttta tatagtctta
     7501 taatacctta ttggccaatt cttgtaatta tacttttagc aactttgttt gtggatgaag
     7561 ttcttactat aaaatttttg aggggaaata aagatatagt agaaagataa ctttaaaaat
     7621 ataagttgaa tttagattgt agttaaaaga aagctatgtt aaaatggtga aatcatttaa
     7681 aattagcttt cttttttatt tgataaaatt attttataat atagctataa gttgtaaagt
     7741 tttctattaa aattaaaata aagtgtagaa aatggggagt gatttatgat gggtaaagta
     7801 agatttggta ttgttgggac aagcaatata gcaaatatat ttctaaaagc tgcatctaag
     7861 gttaaagact ttgaattggt tgctatatat tctagaaatt tagaaaaagc tagagaattt
     7921 ggaatgtcac atggagcaag tatttttttt gatgatttag aacaaatggc taaatcgaag
     7981 gatatagatg ctgtatatat agcttctcca aatgcattac attcaaggca agccattatt
     8041 tgtttaaaac acaaaaaaca tgtactttgt gaaaaatcgt tagcatctaa tttaaaagaa
     8101 gtcaaattaa tgataaaaac agccaaagat aataatgtat tgcttatgga agctatgaga
     8161 attacatgtg ttccaaactt tagagcagtt aaagaaaact tgtataaaat agggaaaata
     8221 agaagatttt ttggaagcta ttgtcagtat tcttccagat atgataaata taaaaatgga
     8281 attatagaga atgcatttaa aaaagagtta tcaaatggag ctttaatgga tataggagtg
     8341 tattgtatac atcctatggt aaatttattt ggggctccca aatctgtaaa ggctgtttct
     8401 catgtacttc aaactggagt ggacggagaa ggttcagctg tatttcaata tgatgatatg
     8461 gatgctataa ttcagtattc taaagtagct gattcttata taccatctga aatacaagga
     8521 gaagagggaa gtataataat tgaaaagctt aatctatttg aaaaagctat tattaaatac
     8581 agagatggaa gagaagaaga tatatctatt ccaaaagaaa aagcagagga aaaacctaga
     8641 gaaatagaag gaatgtatta tgaacttgta gagtttataa gtcttataaa taacaataaa
     8701 atagaatcaa atataaattc ttatgaaaac tcaataatag taatggaaat tatggatgag
     8761 ataagaagac aaagtagtat tgtttatcca gctgatagta tatagtttat attataaagt
     8821 atataagaat ttataatata tattcttaaa taaatttaat tttaagttaa ggtaagatgt
     8881 atacttagtt aagtgttaaa atgtcaaatt ataagtatga aaaagtgcta aaaagaaatt
     8941 tatttttggc gctttttttc ttttttttac aattttgtaa cagtatcaaa aaaagttttg
     9001 acgattttat tacaattttg tagttgaata tacatatcaa aactaatatg attaataata
     9061 acataaattt gaatattttt agaggagaga aaatatgaga aaattgatat actttattac
     9121 accttttata ataggcgtag tatttttatt tggactagac aaatttcttg attctaaaac
     9181 tgatgaactt ttgagggaga aaaatctact tccaattatg gatgatactt taagtgatat
     9241 aaaagataaa ggagttacag caaataatca ttttttaaga gaaaaagata ttatgatttt
     9301 ggggtcatca gaacttagta attcaacaaa acaacatcca aagtactatt ttaatacgaa
     9361 taggagtaaa aataaggtgt ttgcaattgg gagagcatat actcagacat tacaagatgc
     9421 tgcaatatta ggaagtatga atcctaatat tgataataag aaagttgtct tgttaatttc
     9481 tatgcaatgg tttatggaaa aagatggagt cacaagtcac cattatcagt ctagattttc
     9541 accaatacaa ttttatcgat ttttagataa tccaaaaata tctaaacaaa ataagattga
     9601 gtatgcaaaa aaatcaagta agttattatg gggaagtgac gagtataaag cagaagcatt
     9661 atatgctaag ctatatgagc ctaagacgtt gcttgaaaaa gctgaaaagg tgcttttaga
     9721 gccttatttt caaggaagaa agtactgtat agctcttaaa gaaaagggta ttctttataa
     9781 acgattaatt aagctagata aaaagagagc aacaaaaaga aaaagcccaa taaattggtc
     9841 gcatgagaga aagaaagcca ttgaagatgc taaaaaaaga gttggtaaaa atccactaaa
     9901 tattgataat tattattata aacagcattt taaagatggt atagatcaat acaaaggaag
     9961 agacaaagat gtaaatttat taacatctaa ggagtttgaa agttataaac ttatgctaaa
    10021 tgtttgtact gacttaggta ttaagccagt tgttgtatta atcccaagta tggataaatt
    10081 ctataatttg actggtattt ctgaaaaaga aagaaatcaa tactacgata aagcacaaaa
    10141 catagcagaa agcaaaggct ttgaggttct aaatcttaaa gataaagggt cagataagta
    10201 ttatttaagg gatgtaatgc atcttggcac gaaaggatgg gtagatgtct gtgagagatt
    10261 atttaaaata tttaaagaac aataaggatt tgttaactag tatttgttta actatggtta
    10321 taatattggc gataggagtt ctaacattta ctccatttaa agaattgcca tttatttata
    10381 acggatttta aaggagcatt cttatatgaa aattattgaa ggaataaaga aatattcaaa
    10441 tacagataga atagcactaa tatgtaatgg agataaactt tcttataaag atttaaatga
    10501 gtattcagat gctatatcaa tatttttaaa agatgtatac aaagaagaag atactcctat
    10561 agtaatttat ggaaataaag aaaatatgat aatggcatgt atgataggag ctttaaaatc
    10621 agggagagcg tatgtgcctc ttgatattag ttttccaatt gatagagttt ttgaagtcac
    10681 taaagagata aaaccaaaag tattatttaa ttttagtgat gaaaaagatt ttggagatat
    10741 taatgtaatt gatatggata aactaaagga tataataaat gaatatcagg gaaaatcact
    10801 agataaagaa aactgggtaa aagatgatga aaatgcatat atattattta cctcaggaag
    10861 tacaggaaaa ccaaaagggg ttcaaataag ttcaaacaat ttggatagct tttcggattg
    10921 gataagccca tatctaaaca tagatggaag tgaaaaagta ataatgaatc aaccagcata
    10981 ttcttttgac ctttcagtta caacaatata tcctggtctt atccatggag ctacattatt
    11041 ttcaatatca aaagatgtat tggcagacta taaagaacta tttagacaat ttagtatatc
    11101 agatatagct gtatgggtgt caactccttc ttttgcaggg gtatgtataa ctgaaaaaga
    11161 gttcaatagt aaaatgcttc ctaagcttga atcaatgata ttcataggag aagcactttc
    11221 aaaaaatctt actaaagaac ttatgagcag atttccaaac acaagagtta tcaatggata
    11281 tggaccaaca gaggctactg ttggtgtgag cgtaaatgat atgactcaaa aagctataga
    11341 ggatgaaaaa agtcttccag ttggatatcc tatgtcaaat tgtaaaataa aaatacttga
    11401 tgaagctgga aatgaactta aagaaaatga aaagggtgag attataataa ttggaccatc
    11461 agtttctaaa ggttacttca ataataaaga aaagactgat gaagtatttt tctatgatga
    11521 aatagatggt ataaagtgga gagcttataa aactggagat atgggatatc ttttagatgg
    11581 aaatatatat tattgtggaa gaaaagattt tcagattaaa ttaaatggat ttagaattga
    11641 aatagaagat atagaaaata atttgagaaa agtacataat gtaaaaaatg tagtggtttt
    11701 accagtatat aaggacgaaa aaattgctta cttaaaaggt attgttgagt taaatgaaaa
    11761 gaatgattta agtaatatta aaaatgggat gataataaaa aaggaacttg gaaagtatat
    11821 accttcatat atgattccaa gaaatatttc aataatctct gaatttccaa caaatatcaa
    11881 cggtaaaata gacagaaaga aacttatgga ggaaatttaa tgattttttc acagtatgga
    11941 gattattttt atctttatat attattatta acatcaatac cagcagtaat tttaggtcta
    12001 atgggtaaga atataaagta ttatggtatg ttggcaagtt tgtttatgat attcctaata
    12061 gtaggaattg atgtccaact aaagtactta gtaatattta ttattcttga agtaataata
    12121 gttaaaggat atgaatatgt aagaagaaaa actaaaaata aatacgtata ttggagcttt
    12181 ttgtttatat caatgcttcc aatcataatc aataaaatat ctccagttac ttcttttgga
    12241 atgattggat ttattggaat atcatactta aatttcagag ctatacagat ggttattgaa
    12301 atttatgatg gtgctataaa agaagttaaa atttcaaaga tgctttattt tatgttattt
    12361 tttccaactt taagttctgg acctattgat aggtcaagaa gatttgaaca agacttggaa
    12421 aaacaaattt ctagaaaaga gtatatagaa gaatatttac tgccaggaat aaaaaatatt
    12481 gtcatgggag taggttataa gtttgttata gcatttttga taaatacata ttgggtatca
    12541 ataataccta aagatatgag ttttataaat attttaagtt atatgtacgc atatagcttg
    12601 tatctattct ttgattttgc aggatatagt ttatttgcta taggtacagg ttacatattt
    12661 ggaatacaag tacctataaa ctttgacaag ccatttatca gtaaagatat gaaagagttc
    12721 tggacaagat ggcatataag cctttcaaga tggtttggag attatatttt ttctagattt
    12781 gttatgtcat caatgagaaa gaagagattt aagaaaagaa ctactgctgc tcatgtggct
    12841 cagatgataa ctatgataac aatgggattt tggcatgggc taacttggta ctacgttgct
    12901 tatggtgtat atcaaggttt ggcacttgtt ctaactgata tatatcagag aaaatccaaa
    12961 ttttataaaa aacataaaaa agaaaaatgg tttgagagag tacagatttt cattacattc
    13021 cacattgtat gttttggatt actgatattc tcaggttatt tagcttaact ttaaggagga
    13081 aaaattatgc aagagacagt tatagaaata tttgaagatg tattagggac tgatgagata
    13141 agagaagatt tagaccttaa cttatttgag acagagcttt tagactcatt agctataatt
    13201 gaagttttac ttgaaattga aaatagatta ggaatagagc ttcaaccaac tgatttagag
    13261 agaaaagata tgtctactgt aaataattta gtaaaattct tggaaacaag aaaataaatt
    13321 agtagttaga ttaaatatta atttataaaa taaaatttta caaagtccat ctgatactaa
    13381 tatttgatgg acttttttaa tgataataca tagtcttttg aagacaactt taattaagta
    13441 aaaaatttaa tatataaata tgattttgta aatcttaggt atcattgtaa tattatagga
    13501 gaaaaatata ttatatttag gaggaaaatg aatgtcttta aattcaatag atagaaaaaa
    13561 agaaatactg aaattattag attcaaaagg taaggtaaat actaaagagt tagtaaaaag
    13621 actagaagta tcatcagaaa caataagaag atatttggaa gaattagaaa atgaaaataa
    13681 attaaaaaag gtatatggtg gtgctataaa aataaattat ggggtgattg aaccagaaca
    13741 tataaagaga aatagtacta atttagataa aaaacaaagt attgctaaat atgcatctaa
    13801 gtttgtgaat gacaatgagt caatcatcat agatgaaggt actaccaatt tgcaaattgt
    13861 agattatata atatctgtaa acaatttaaa aattattaca aattcttatc cagttctatc
    13921 aaaattgatt tcttatgaaa atcaaggctt atttgatggg gaagttatat ttattggtgg
    13981 aaaggtaaat ttaaaacatc aaagaacagc gtgtccaatg tcaatagata ctatgaagaa
    14041 cttatacgta gataaagctt ttatatctag cgaaggtata cttgatagtt ttggaattag
    14101 tagtatagac caaaataagg cattgttgtc aaaagaatat ataaaaaatt caaaggaaag
    14161 ttttgtattg tgtgatagtt ctaaaatcgg aattgctagt ctgtataaga tagcagattt
    14221 agaggaatta gattttgtta tttgtgatgt agagcctccg aatgaatgga aggataagtt
    14281 aaatgagata aatataacat ggatagaagc aaaataaacc cttagtttta tagaatacaa
    14341 ctatgcttaa tacaaccaaa ttcataaaat atagtcagtt tatagattaa tattagaaag
    14401 taaattttag attgtaaatt tagaatttta tagaaatatg acatagaata aaaacaactt
    14461 aaaacaaata aaaacaacta aaaacagaaa tttaatatat atttaactta aatatgatgt
    14521 ataattaaca tatgaaagtt atactttaaa agtaaaaaca attaaaaaca caaaaattct
    14581 aaaaagaatc tatggaggct ataaatatga aaaagatata tggagaaaaa ataaaagcag
    14641 tagtattcga ttgggctgga acaacagtag attatggatg ttttgcacct cttaatgtat
    14701 ttatagagat atttaaaaga agaggcatag atgtaacaat ggaagaagca agaaaaccta
    14761 tgggaaaatt aaaaatagac catataagag aaatgtgtga aatggataga ataaaaaatc
    14821 tttggtcaga taagtttggt aaagtgccaa ttgaagacga cgtaaatgaa ctttatgctg
    14881 aatttgagcc aatgttattt gagacattag aagagtatac tactccaatc ccccatgtta
    14941 ttgaaactat tgaaaaatta agagaaaatg gattaaaaat aggctctaca acaggttata
    15001 caagagagat gatggatata gttcaaccaa acgctgcaaa aaaaggatac tcaccagatt
    15061 ttcttgtaac accatcagaa gtatcacaag gaagaccata tccttggatg tgctataaaa
    15121 atgctgaagc tttaggagta tctcctatga gttctatggt aaaagtagga gatacaataa
    15181 gtgacgtaaa agaaggcgta aatgcaggaa tgtggagtgt tgctgtaata aagggttcaa
    15241 gtgagttagg gcttacagaa gaagaagtag agaacatgga taaggaagaa ttaaaaatta
    15301 aaatgaatac agtttcaaag aaatttaaag aagcaggtgc acattttgta atagaaacta
    15361 tggctgaatt agaagatgta ttggtaaaga tagaaaataa aactattaaa tcagactttg
    15421 taccagaaaa tgattacata ttacttacac cagggccact atctactact aaatcggtaa
    15481 gagcatctat gttaaaagat tggtgtactt gggatgttga atataatgac ttagtacaag
    15541 atgttagaag aagattaatt tctttaggaa caaagaatac agacaaatac acaagtgtat
    15601 taatgcaagg aagtggtact ttttcagttg aagctacaat aggtagtact attccagaga
    15661 atggtaaact tcttgtaatt gcaaatggag catatggaaa gagaatgaaa gatatatgta
    15721 attatcttaa catagaattt gttgattgca catttaaaga tgtagaagcg gtggatttaa
    15781 atgtagtaga aaatttatta aaagaaaata aagatataac acatatatca atggttcact
    15841 gtgaaactac tacagggaga ttaaacccca tacaagaggt agggaaactt gcaaaagaat
    15901 ataacaaagt ttatatagtt gatgctatga gtagttttgg gggaattgag attgatgttg
    15961 aagattttaa tatagatttt ttagttagta gttctaacaa atgtatacaa ggagtacctg
    16021 gatttggttt tataatagct aataaagaag aattatctaa atgtaaaggt atagctaaat
    16081 cattatctct tgatgtatat gcgcaatggg agactatgga gaaaaataat ggtaaatgga
    16141 gatttacttc acctacacat gttgtaagag ctttttatca agctttatta gaattagaag
    16201 aagaaggttc tgttgaaaaa agatatgcta gatataaaga aaatcaattt acaatagctt
    16261 caagattaaa atctttaggt tttgatactt tagtgaatga taatgctcaa tcacctgtaa
    16321 taacaacttt cttatatcct aaaaatgcaa agtttgaatt tatggaattt tatacttatt
    16381 taaaagctaa tggatttgtt atctatccag gtaaattgac tgatatagat acatttagaa
    16441 taggtagcat aggtgaagtt tatccaactg atatggagag actagcagat gtaatagaaa
    16501 gatttatcaa tagatagaag aagatagtgg cttgtaggtt actatagaat ttaaagactt
    16561 atattttaga tgtttataaa atgaatatat aaagattgag taatagggat atattttact
    16621 taaaccacaa tctaagaaat tagatttata gtttagataa agtatttttc ctattatttt
    16681 ttgtgtttat ttatataaaa taaattatgg ttattttatt aagtattatt atcatacttg
    16741 acaacgtaac aatgttttgt tatattaagc ttagagataa aatacggtaa agaaaggata
    16801 tcattatgaa tgaagaatta agttcactaa gatttgatgg cgttggaaaa tgtaaaggtg
    16861 gaatttttga taatgttaat atagcaggaa caggaaaaat agaaggaagt gtaaaatgta
    16921 gtaaatttaa ttcttctggt tcagcaaaag tgtttggtac aacagaatgt aatgaatttt
    16981 ttacatctgg tttaagtaaa gtagaaggaa atatactggc atcaaaagtt gagatatcgg
    17041 ggcttcttag atgtacagga gatattaatt ctgttgaaat tgacactagt ggaattatta
    17101 atgtagaagg aagtataaag tcggatatta ttcgtggaga aggatattta aaagcaaatg
    17161 agaatataga ttgtgaaaat atcaatttaa gtggaatcat ggaatgtaat ggatttttga
    17221 attgtgaaga ggttaatatt agtttaaatg gaatttctaa ttttaatgaa gttggagcaa
    17281 gtagtataaa tataaaaaaa ggtcatggag attattgcaa aaaatttcgt atgctagaaa
    17341 aaaagcatat caactttgaa aaggtatctt caaatttaat aagtttgggt ttattatcaa
    17401 ttagaaagta tatagaaagc aaattatttg caaatattat agaagcagat gaaataagcc
    17461 tagaaaatag tgaagtcaaa gttgtaagag gaaaaaatgt taaaataggt aagggatgtt
    17521 atgtagaaag cttggagtat agtgaaatta tagagattga tgaagattca atagttggtg
    17581 aaataaagaa tataaatgaa aaagtaaaat taaagaagga taagtaataa gtaggtttaa
    17641 aaataaaatt taaaatgagg taataatatg gaaaaagggt ctataaaaat acttggagaa
    17701 ggctttataa gtagtggtga gtatgaaagt ataagaatcg tgggaagtgc taaatcaaaa
    17761 ggaaatatca aaagtgatga agtaaaagtc cttggagatg caaaatttga aggtgacatc
    17821 aatataaaaa attttgttgt taatggaaaa actattataa gaggcaatct taaagcagag
    17881 cgtgtaagag tgaatgggca acttagcata tatggagatg ctgaaataga tgacttagtt
    17941 ataactgctc aacagaaagt ggatggcaaa cttaaatgta ataatgttac aataagagga
    18001 caattaagtg taagtgaaaa tatgtatgca caagatataa aaatctatgg cgaaatgaga
    18061 aataaatcaa atatagaatg tgaagatata aaggtatatg gagtaataga atgtgatggc
    18121 cttttaaatg gagaaaatat atatgtttat tcaggaggag gttcttattg taaagagata
    18181 ggtgcgacta atataaaaat aggaagaagc aaagatgttt tttcattaat taaaattatt
    18241 aatatttttt caggaaagtt taaatgcaac ttgatagagg gagatactat tgaattagaa
    18301 gatgcatcaa taaaagatat acgaggtaaa agcatatttt tagtagataa ttgtgatgtt
    18361 ggaaatattg aatacagtga taatttgtca gtttcagata attctactgt aaaaaacagt
    18421 ataaaggtgg gctagaagag ttatggaaga attaatatct aagaaagatt tattgagcaa
    18481 aactaatata tcttatgggc aattatatag atggaaaaga aaaaacataa taccagaaga
    18541 atggtttata aagaaatctg cttttacagg tcaagagact ttctttccaa gagataaaat
    18601 actagaaaga atagagttaa tattgtctat gaaagaagag acttctcttg atgacattgc
    18661 aaatatgttt atgaaaaaag atattaataa agaatttgat attgatttta ttcttagtaa
    18721 agaagttata tgtaattaca ccaaagaaat atttcaaaat ttatataaga aagagcaaag
    18781 tataggtaaa aaagaacttc ttatattgag tataatagag aagtttttag ttaaatctac
    18841 aataactttt gaagagttaa aattgatagt gaatataata gaagaaaatt ttacatcaat
    18901 atataatgaa aatggaaaga tttatttgtt tagaaagttt ggagttccat ttgtagttgg
    18961 atgtctggat tataaaaaag taaaatttga aaaagatgta gttaagatta ttgagattga
    19021 cttaacaaaa gaaataaatg atataagtat aaagttatcg tagtgtgtat tatcttatat
    19081 aatgtattac aataatctac ctaaaataca ctttatttaa atattgggtt taaagatgag
    19141 tttttatgtg aatatgtaaa acttaaagat agatttaagc aatctatagg gattagtaaa
    19201 caataaaatg aaccatgatg aagtactata aattgtattt aaactacatt ttctacttat
    19261 agtaatatca atggttcatt ttatttttat taagataata cagtatttat aaaaatactt
    19321 aatataaata tagcaaattg tttaaataaa atgattatct tagtttattc aaataatgag
    19381 tttttaaaat ctaagaaata tccttgtgga tgaagacata caggacaagc ttttggagct
    19441 tctttaccta catgtatatg cccacaatgt gtacatatcc attgtatatc ttcatttctt
    19501 ctgaataaag taccttcttc taaatcagta gcatatctac caaatctatc accatgaact
    19561 ttttctattt cagctatatt ttcaaataac ttagctatag attcaaatcc ttcttcacgt
    19621 gctgtttttc caaagttttt atatacatca tcccattctt gaagctcatt atgttgtgca
    19681 gctttaagat gttgtgctgt tttatcatat aaatctacag ggtatgcccc tgatatattt
    19741 atgttttcac cagcaaattc ttttagttta gaaaagaatt gttttgcatg tgatttttct
    19801 tggtcagcag tgtaattaaa taaagattct attatatata atccttcttt ttttgctaca
    19861 gatgcagaaa tattgtatct atttctagct tgactctcac ctgcaaaagc tctcaataag
    19921 ttttctttag ttttactatc ttttaaattc atattctacc tctctatcac aataaatttt
    19981 tacaatattt gattcttttt atttattatt ccaatagaag atataattat gcttaattta
    20041 gtcagcattt ttaaaattat cattaatttt ttttaaaatt ctaagaaatt cttgtttttc
    20101 attttctgta atattatggt atgctgtttc tttaacttgt gttgagatac tattaaatgt
    20161 atcttctatg agtagtgctt tttttgtaag ctttatatat gtgatacgtt tgtcattggt
    20221 acatttttgt ctttctagat atcctagatt tatgagttta ttaaccaaca ctgtaacagt
    20281 ggatttgtcc tttcctattt tagttgcaat ttctttcatt gtaagtattc cattattttc
    20341 ataaagtgca gttaagatat ttccatgagt aggtatcaac tcatctaaat catttttact
    20401 gagtttattt tcaataaatt taatcatatt agtctttgtt ttacttatga aatgtactat
    20461 atatttatct tccataaatt attctccaat ctttaatcta tttaaattag tttgacataa
    20521 aactatttat aaactataat tagtttaaca taaaactaaa tgtattggga ggaaatttat
    20581 gaataaaaaa tttaaatcac tatatattac agaagaggaa ggaaatttta aaagaaatat
    20641 agttgaaaga gatgtagata cattgccaga gggagaagtt ttaataaaag tgaaatattc
    20701 ttctctaaat tacaaggatg cactttctgc caatggtaat aagggtgtta ctagaaaata
    20761 ccctcataca ccaggaattg atgcatctgg tatagtagaa tctagtagtt gtgatgattt
    20821 taaaattgga gaagaagttt tagtaacagg ttatgacttg ggaatgaata cttctggtgg
    20881 attttcagaa tatataagag ttccttccag ttgggtagtt agacttccta aaaatctttc
    20941 actaaaagaa gcaatggatt atggcacagc agggtttaca gcagctttat ctgtgtacaa
    21001 atttatagat tctgtagaca aggacatggg agatgtatta gtaactggtg gtactggagg
    21061 agttgctgta attgcaacca aaatattgat taaattaggt tattcagttg tagtttcaac
    21121 aggaaaacta gaagaacaaa aacaagcatt actaaactta ggtgttaaag atgttataca
    21181 taggtcagaa gtggatgata actcaggaag acctctactt agaccaagat gggctggagt
    21241 aatagataca gtaggaggaa ataccttagc aacagcaata aagacaacta attattgtgg
    21301 agcagtaaca acttgtggta atgcaggagg agttgatttt acaagttcag tatatccatt
    21361 tatcttaaag ggaattacct tgtatggtat agattctgtg gactgcccaa tgaatttaag
    21421 attaaaaata tgggatttat tatcaaatga atggaaatta gatgatttag acgatatggt
    21481 aaatgtggta tcattagaag aattaaatga cagtatagat tcaataatgg caggaacaca
    21541 tgtgggaaga acagtagttg atttagaaaa aatataggtg actaagtttg caattaggat
    21601 tttgggtttt aagcaaaaat gagatacttg taatttactt atgagtatct cattttttta
    21661 tgtaaaaaaa ttttatgtag aagtttgtgt aaacaggtta ttcatgaacg attaattcaa
    21721 atacatctgg tcttgaatag tgcccttctg aatcaaaatc tagtctgcta agaactattt
    21781 tttctaggtc taaatcagct attagcattt cttctttatt gaaaataggg ccagctacat
    21841 attttccaaa agggtcaact atacaactcc ctcctggaca cataatttct ggttctgcat
    21901 ctagttcttt atagtaatta aggtcagtag gatacatgtt tttttctaca tattgattac
    21961 atccaattac aaaacatctt ccctctaaag caatatgttt catagttgct tgccactctt
    22021 ctcttgaatc tgcagtaggg gctatataaa gttttacacc cttagcataa agagctgttc
    22081 tagctaatgg catataattt tcccaacaaa tcaatgaacc catttttcca tatggagtat
    22141 caactactgt aagagtactt ccatctcctt ctccccatat acatctttct gttcctgtag
    22201 gtttaagttt tctgtgttta ccaattagct taccttctgg agagaaaaat aagtttgtgc
    22261 aataaagggt acaatttata tcattaccat ccctctctgt tatacctatt gatagatata
    22321 ctcctgcttc ttgtgctgct tttcctaata aatctgttgt agcactagga actggtacag
    22381 aattatcata gtatcttttc caatcctctc tacctttcat tgttctactt ccaacaacaa
    22441 atccaaatga aagacctctt ggatatgcag gtatgaatgc ctcagggaaa actacgatat
    22501 ttgccccttt tctaccagct tctttaatta atcccaatgc tttttcaaca gttttttcta
    22561 aatccattat gactggtgat gcctggacaa cagcaattct tacattttta ttttccttgt
    22621 tcataaaaaa cctcctaaaa aatatttaaa tttgttgcat tgaatagact acatgagatg
    22681 ttatttgaga acgaagatat ttatattgtg aataataata taactgtata tttaaaataa
    22741 tacatcatga tttaaaaaat tacaattgta catatataat tgagtttaga tatttaaatt
    22801 taataactta taatattttt tcaattagta taaagcctat taatttgtct tatttacaat
    22861 gaatagtatt tacttcttgg caaacaataa ttaaaaataa aacttttata taattaaaat
    22921 ctaaagaaaa agtttataaa gaagataata taattaagta gaaaataaag aaaaaaattt
    22981 attaaagtag acaacataag gagaatttta tgaaagaaaa aatttattac aatggaaata
    23041 tcatcacaat ggaagattca atatgtggag atgcgattct tataaaagat aagataataa
    23101 aaaagatagg aacaaaagaa gaagtatttg ctttgaaaaa taaagataca gaaattatag
    23161 atttgcaagg taagacattg atgccatctt ttatagactc acatagccat ttgatagctt
    23221 ttgctactac tctaaagtta gttcctttag aagatgcaac tagttttaaa gacatagtaa
    23281 aaaaaataca ggatttcaaa gagtcaaata atataaaaaa aggagactgg ataattggat
    23341 tttcatatga taataatttt ttagaagaaa ataaacatcc agataagaat gtactcgaca
    23401 gtgcgtcttc agaaaatcca atactaattt cgcatgcttc tggtcatatg ggagttgcaa
    23461 atacattggg gttaaaacag cttggtgtta ctaatgaaac atatgaccca gaaggtggac
    23521 atataggtag agtagaaggc agtgaagaac caaatggata cttagaagaa aatgcatttt
    23581 ttaatgtatc atctaaaata aaaccaccat catcaaatga gatttttaat tcaattgaaa
    23641 aagcacagga catatatcta agctatggta ttacaacagc tcaagaaggt ctaatggaag
    23701 aaaatcaatt taatctttta aaagcaatgg caaatcaaaa taaattaaaa attgatgttg
    23761 taggatatgt aaatttgaaa aaaagtaagc ctgtagcaga taacaataga gagtttataa
    23821 aaaaatatat aaacagattt aaaataggag gctataagat attcttagat ggttctccac
    23881 aaggtaagac agcatggttg agtaaaccgt atgaaaattc agatgatggt tattgtgggt
    23941 accctatata caaagatgaa gaagtagaaa agtttataga tatttcacta aaagaaaaga
    24001 tgcaactctt aactcattgt aatggagatg cagcagcaga gcagttgata gatgtatttg
    24061 agaaagtttt aaccttaaaa gagcaaagta gtaataatat gagaccagtt atgatacatg
    24121 ctcaaacagt aagagaagac caaattgatg atatgaagag aataaatatg ataccttcat
    24181 actttgtagc tcacacatat tattggggtg acattcatat caaaaacctt ggagaagata
    24241 gagcatttaa gattagccca cttaaaacaa ctatagaaaa ggggttaatc tacactcttc
    24301 atcaggatac accagtaata gcacctaata tgctggaaac agtatggtgt gctgtcaata
    24361 gaattacaaa aaaaggtgta caaataggag aaaatgaaaa gataagccca ttagatgcct
    24421 taaagggtgt tactataaat gcagcatatc aatattttga agaagataaa aaaggctcaa
    24481 ttaaagaagg caaattggct aatttgataa tattagatga aaaccctctt acaatcgacc
    24541 ctatgaaaat aaaagatatt aaagtattgc agactataag agaaggagaa gtattgtatt
    24601 ctttgaagta gatgcaagtt agatatttta taatctataa aaagtagttt tatataataa
    24661 gttaggtaga attaaataag attgcagtta aatcaacaat tatattt
//
LOCUS       DAFNNB010000042        24066 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0042, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000042 DAFNNB010000000
VERSION     DAFNNB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 24066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24066
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0042"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            193..648
                     /locus_tag="L3M60_001500"
     CDS             193..648
                     /locus_tag="L3M60_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226178.1"
                     /translation="MGLRDKFAQSFARSKTMSGPEKKANEIMGKLLLKKAILPIVLMF
                     VIIIAGAMLKINSWVTLGINLVIAVGAFFYIRNSSKKYQNFKPYVGNLISLEKKGKKE
                     YVAIIKQGKLPVKLQIAYGGEDLEHVKKNQMVQISYNPDAKIAILVNRQ"
     gene            complement(859..1119)
                     /locus_tag="L3M60_001501"
     CDS             complement(859..1119)
                     /locus_tag="L3M60_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226179.1"
                     /translation="MDKLITELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVQMGIMKTDVEEIKEDVTDIKGMLQNGV"
     gene            complement(1382..1735)
                     /locus_tag="L3M60_001502"
     CDS             complement(1382..1735)
                     /locus_tag="L3M60_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226180.1"
                     /translation="MFKFDKNKIEQIKQGRKVEMQNKDISDISIGQVKQDDDITNNFI
                     ANAEIYEMLLSQSSVNEASNISTFSVRKSGGESGMVEVYVALILRGKKTIEEVPAVIR
                     EQVRIRCKELEIPVE"
     gene            complement(1752..6929)
                     /locus_tag="L3M60_001503"
     CDS             complement(1752..6929)
                     /locus_tag="L3M60_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome condensation regulator"
                     /protein_id="HBN6226181.1"
                     /translation="MKQNKLLQRGAYFNDKNILIDDFDKRYNDYDFVEFFTGISNSTF
                     GLKSDGNLYACGDNTGFQLGLGKDSSERRMFSKVKIDNVKYVSCGSKHSVAVTKDGFA
                     YGAGTSNVGQLGVIESTVYYEFTKLPIYDVKTVACGYDFTFVLKNDGTLYSAGLNSSG
                     QLGLGDTNNRATFTKVNIDSVKDVITYNQSVFIIKMDGTAHACGLNSNGQLGINSTLN
                     KSVFNKIEGMDNVKQIACGSSHTILIKNDGTMYTTGYNGVGQLGTGNNNNSIVFTLSS
                     INNVKYASCGNNHTMILKYDNTLFSTGQNNYGQLANANKDVASRNTFVKVNVENIKDI
                     KCGSQFNFLINGSKEIFVSGCNLAGQLGSFFHTTFLYEFSKVQSSNLDNYSGLLVNDD
                     YLYVTKDNSEFLNVKLSDNFQDYKKIELTDNNMFIVMNDGTLYACGLNNYGQLGLGDT
                     VNRSVMTKVDIDNVLDIKGNGNSTFVLKNNGTLYSCGLNNYGILGLKDEVNRNIFTKI
                     EIENVKEFCVESEYIIALNHSKEVYGWGYSANSNIERTSNYPDKQNINGIEKIATYYR
                     SVYMINSEGKLYVSGYNSYYQLGTGNSSSTNKTLVSKYRTVTLSTSSSNLSDLPKIVN
                     VFPFNYGCAIIDKSGFVYLAGQHRYPDTSKSNPSINDYSRYGSFIETTSSYYNTYFTQ
                     VNAFNGIEKVIGTSNNILYFKKGSTYVTGYPKTFGSSATGYKSYTTISSEYSNLGSLF
                     IITSSSSKLYGHGVANNGEFGNITNLDGVSSYDTGLKDIKDIIVKNNTVVVVDKNNNI
                     YVTGTNQFNKLGIGEYNNQPIRKFTNITEQSNSFIFMDDIKEITTSRNTMFIVKNDGT
                     AYATGNNSSGQLGLGDIINRNKFTQINLDNIKKISTSIDGNTTFAIRNDGALYSTGLN
                     TKGQLGLGDIVNRNTFTKVNIQNVRDVVLGTTHSHAIKDDNTLYSCGENTHGQLGLGS
                     ESNHPDVLTFTVNNITNVRDVYCSDTTTFIVKDTNIAYCCGYNNNSQLGMGNTTDQYS
                     FIKCMENVKEVIPNEINTYIITIYNTAYSTGLNTDYCLGLNSNSNQSSFSEIPISNVV
                     KVAPNRNNAVLLLTSEGDVYTAGKCSNGSGTGSETPEKIKKIASKAKDIGMNYRCGHY
                     VSDNGDLYGTGFNDCGQLGVGDVTKRDTFIKTNTRVKKILPLEYANIAIKDTNDIYIC
                     GLNNYGQLGVGNRYDSRNNDNRIFNYKHMNFVMGDLTSIKNRHNFILLNNKIVIPTTK
                     DIDYGLVLGNLYKGDLYTELPYENIKEVSISKTHIIILLNDGTMYGCGTNYHGELLQD
                     LSINQVDEFVQINVSDVKHVSCGDNFTYFIKSDDSLWSIGKNSEYQLGIGHNNPVTEL
                     QRITTVSSCKEVHCGKNYTLVVTTGNELFVQGYNDKGALGLGSDNENAIIKFFTKALT
                     DIREIKSYGSDHILVLKNDNSVWVTGKNRDVYKIEQPVEFLKEFTIVPISEDVNTVKD
                     VLATDNTLYIISEVGTTNAAIEITEKSISSIKIKIQDPNKDISRIEMLINGESVKSVS
                     DLTTEKISFEVPPDKIKIGENKILFRAYCKGDDLYASLFIFKESTGNSIIKDSYVMIG
                     NRMYKVVNTTSNEQDITITLDRGLEEDLNLGDPIYQLINKTKVQVKINKSDLFKDMKL
                     VEIKKSDSSYQEIYELEEANIKSAQPKIIVEKGDKWTAIKRPSMIFRYDAENNEPQA"
     gene            complement(6951..7937)
                     /locus_tag="L3M60_001504"
     CDS             complement(6951..7937)
                     /locus_tag="L3M60_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBN6226182.1"
                     /translation="MEEKFYIILTKIGREKIANATALGELVGLTKFQVGDSNGEYYEP
                     TEEQTALKNVVWEGNINSLRIDEKNPNWIVIETILPGTVGGFMIREAAVLDNENNIIA
                     IGKYPETYKPRAEDGSIKDLVVKMILQLSNTSNVTLEVDPTLVFVTQKDIQDLDDKFD
                     KNIKEIKVNIGDVNILTTDSKDLSGAINEVVKKIENISFDDVISGQIQTDISVLKNSY
                     NKLSEKVLDILIYLELESEVTVDEAGYWYDTLTNGNNIVAIEGLKLDLNRKCITGEIG
                     NVIFRDVVLPFSANRVRYIHDMDNNFVETKSSNTYLKEQKDITLSKYSYEIR"
     gene            complement(7938..8636)
                     /locus_tag="L3M60_001505"
     CDS             complement(7938..8636)
                     /locus_tag="L3M60_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBN6226183.1"
                     /translation="MKLIDKLPSFDRNYIVEEIQGAYDTELNILKEDIDDTFNQLFVD
                     TATWGLDMWEDILCIEKKELDFDTRRSNIKAKMRSRGTSTIEVIKSICEAYTKSETDI
                     KVYSDEFTFVLSFIANNCDYKTLLDCSDMIERVKPAHLLHYLEPIILDKSIVYCGGGM
                     VCSEEVKVHPYFEPIIKCSAVVNCGAGMLSREEIKVYPLSIKCIENNCKINIAIANDT
                     GVENVVVYPKSEVV"
     gene            complement(8633..9685)
                     /locus_tag="L3M60_001506"
     CDS             complement(8633..9685)
                     /locus_tag="L3M60_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBN6226184.1"
                     /translation="MYSDQTYEVIKNRTLENINLDIYKGEGSFLNNMISGNNLELSKI
                     YLELSKIHKMAFIQDTYNQFLDKRVNEFGVYRKLGTESNGEVEFIGENGTVINNGTVI
                     SYRDLLFVVIKDVTIGSEEGDNSPVQALEVGKRYNLPTNCEFKLVDNISGVTKITNTR
                     SFEGGTDIETDEELKERFYKIQRNQATSGNKAHYEEWALEVDGVYNVKVYPRWDGPGT
                     VKVLIFGKNNQAVDTETIERCQQHIDEEKPIGPTITVVTPLPIEINISAVMKLEDGYT
                     LDNVKESFLESINTYFRDIRGEIIYTKVMGILINTAGVHDLSNLLINGSTDNITINED
                     KIPSVTTVNFSEVENQ"
     gene            complement(9678..10106)
                     /locus_tag="L3M60_001507"
     CDS             complement(9678..10106)
                     /locus_tag="L3M60_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2634 domain-containing protein"
                     /protein_id="HBN6226185.1"
                     /translation="MSTIFPFIGVPEDYILPKTEELPIFREVAWDFEKDEPILEKGDF
                     KIIEKKEALKVWIYKCIKTNRYEHEIYSLEYGTELSELIGQKYTKGLTESEASRFIKE
                     ALLINPYILEVSVRSANFNRDILSANVKVSTIYGEVEINV"
     gene            complement(10106..10432)
                     /locus_tag="L3M60_001508"
     CDS             complement(10106..10432)
                     /locus_tag="L3M60_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2577 domain-containing protein"
                     /protein_id="HBN6226186.1"
                     /translation="MANPINEFIGIIREEGKHHNQPSFFIGKIKSKLPDLKIETNNII
                     LEKEDILIDSWMLDRQIETFDTETNQEHKHEVKNPFIDTFESGDMVIMFRIGEKFAIV
                     SKLVSL"
     gene            complement(10448..11977)
                     /locus_tag="L3M60_001509"
     CDS             complement(10448..11977)
                     /locus_tag="L3M60_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBN6226187.1"
                     /translation="MIINRSKDSSSNEISFVSKDMGFLLMQSEVSYNFKDKLVEDIAK
                     QVFAENRISVGTIAKTNVKYTKMFIGVNGYDTIMSAYTEASKKTKKKYMIEANLDKFN
                     VIEKGTVTLSVMFEEGFNIINTTFSESMENVKNKVIVVDQYGSKISEKIDNEIFKEVN
                     VIMQKVIQQQENQDVDIDSEFNGIEKSCSLKGYGDVSCITGRGVKVKDSYTKLVGLFY
                     IDTDKHTWQNGEYQIELELNFQNLMDEKSAGQDEPKEESNLGGEGYAGGKEFTAEFTA
                     YCPRKEEGGDTDCRKKKLDPSKKTCAAPMVGKYEQTYYTKEFLNKHPLLNYGDEIQVI
                     TGVSGRDGVYKVNDVGPAITIEKNGTYHIDILFGNVEEASKFGRRKGKIIIGGYSGNV
                     SDKAKIVISEAKKHLGKPYKWGGNGPSSFDCSGLMVYCFKKVNVSLPRTSNQQSKKGK
                     KVEQKNLQAGDLVFFHNPVSHVGLYIGNGEFLHAPQKGDVVKISKLSSRKDFNTARRV
                     L"
     gene            complement(12259..12681)
                     /locus_tag="L3M60_001510"
     CDS             complement(12259..12681)
                     /locus_tag="L3M60_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226188.1"
                     /translation="MEMWLRQAEDRFRFPVFPSSFSINGKATVNSSSILKIGEIATFG
                     GVALKSISISSFFPNKDYTFCDYTGFPSPYDCVNKIEKWMKEGFILRFTITETNINME
                     VIIEGFSYEERDGTRDVYFTLDLKEYKRIKIPKVTPKQ"
     gene            complement(12681..15134)
                     /locus_tag="L3M60_001511"
     CDS             complement(12681..15134)
                     /locus_tag="L3M60_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226189.1"
                     /translation="MGNVREEGINMYLTDNYTPKMNQIISVTDNFRRATVAVSLSTNV
                     MASSIKNSIGSASSRVNSLNSSLRKVQTTASSVSSTMTKLSSSINAVSGVIGSLNGSI
                     MRLAITIAMIIDYFNKLIQKKNELNSNIMIILIFKAKSDEIEKTKNKLLGNLKKIGGK
                     VWKIVIKAKDMTKRVISSILGNLKRVEKRPYQGSINLKDMVSSAMGRILPKLMLFKNT
                     FWSGVIAIKDMASSIISKVFPKLRLFAGKVWRGAIAVKDMASGILGSIKGKISDLTNG
                     ATIGVAVKKGVDLLGQEQNQKVVLESVMKRNTGKVNQRDVDNYYEDLLDIADKTPFDP
                     EDVVAMGTKAKMISNITGGKKEKDITQAMVDVRALNMNTSSEQDVSAAFLSAAKGNME
                     SLNTLVGENYKTFDEALEGISVKQMGLAKEMSNTIPGIISGAQTSINNGLKSIVKPFD
                     DILGQGLKKIKTFIESGLGNLAGLSEKMAGKIGNVMNGKIIIGNKYDQMQSRSVKNGK
                     EFSDSTQYRISNEAEKRKMMVENKQERFENHAATMIGNAPKAIVNAGSTLLQNIDFTA
                     LIDSLLPVVNLVNNLLDSINNKSPIAQGLISIFGTIVTTAFQLIGPVVEAVSPIITRI
                     FTFLGEYAPQINNFIETLGVIWKTVWETLGPLLETGWKIIEPILGAFFNILDKVCKIV
                     KDICKWWQTMINKIKNGSITGTVLNLVEKSKKNYKDNPYAGTKAGDSGKAYSSKKGNN
                     AFGLNYVPYNDYQTRLHEGEMVLTKQEANQYRSRKNGGNINIAKLADTIVIREEADIE
                     KVTSKLVSSIKMAQLGGVL"
     gene            complement(15134..15301)
                     /locus_tag="L3M60_001512"
     CDS             complement(15134..15301)
                     /locus_tag="L3M60_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226190.1"
                     /translation="MYYYWKKKGIRPSLFYAMDKGELKLIEAFFALEIEEEVEKMKHG
                     YGVCPLTGGGM"
     gene            complement(15328..15774)
                     /locus_tag="L3M60_001513"
     CDS             complement(15328..15774)
                     /locus_tag="L3M60_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBN6226191.1"
                     /translation="MSENGLSKNINIVDLLLNSDTENLERPSTIVELKRLSTIFGQEF
                     KVMCRALTISKDEEIQNTCLKIDENMKTDIDLPEMQMLTIIEGVCDLDGKLLFKNKEL
                     MDKFKAPTPKELARKLLLPGEITNLYRILQDVMGYGKNAVIEEVKN"
     gene            complement(15888..16316)
                     /locus_tag="L3M60_001514"
     CDS             complement(15888..16316)
                     /locus_tag="L3M60_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBN6226192.1"
                     /translation="MANMEARNVMSGTWGELWLDGNKVAEVKKFQAKMEFTKEDIIIA
                     GQMGTDTKYMGYKGKGSITLYHVSSRMHKLIGEKIKRGSEPRFVAISKLNDPDSYGAE
                     RIAVKNIAFDDLTLADWEVGVKGEIEAPFTFTEYDFLDII"
     gene            complement(16330..17394)
                     /locus_tag="L3M60_001515"
     CDS             complement(16330..17394)
                     /locus_tag="L3M60_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBN6226193.1"
                     /translation="MAIGLPSINISFKELATTVKERSARGIIAMVLKDAKALGLNEIH
                     EKEDIPVDLSAENKEYINLALMGNVNTPNKLLVYVIEGEADIQTALDFLETKEFNYLC
                     MPKAVEADKTAIKNWIIKLRDIDKVKVKAILGKVVGNHEGIINFTTEDVLVGEKKYSV
                     DEFTSRVAGLIAGTPLSQSVTYTKLSDVVDIPKMTKVDAESRVNKGELILIKEAGAIR
                     IARGVNSLTELTAEKGEMFQKIKIVDTLDIIHSDIRKVIIDDYIGKVTNSYDNKCLLI
                     VAIKSYLEELEKSALIESDSTVEIDFEAQKSYLKSKGVDLSYMTLQEIKEANTGSKVF
                     LKAKIKVLDAMEDIDLSIEI"
     gene            complement(17399..17842)
                     /locus_tag="L3M60_001516"
     CDS             complement(17399..17842)
                     /locus_tag="L3M60_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226194.1"
                     /translation="MLKYKEILETIIEILKKNFTESIFIDDESVQGSEESCFFVSILS
                     VICTPIMLNTNNKDIVISIKYLPKPQSKSIRMYEISDELNKLFNRNIKVTDRKLNITK
                     LEQSIKKEESIYALNFSVTLNYLDSVYEEDVVYENMEEINLNLGE"
     gene            complement(18091..18531)
                     /locus_tag="L3M60_001517"
     CDS             complement(18091..18531)
                     /locus_tag="L3M60_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226195.1"
                     /translation="MENKKDILFKETDERLHNYKYLDIKIKNINLDIKRYENEYSGCG
                     AMVYTEKTSNTYNISSSVENEVLKREERLRKLKIEKEDIEIEKEKIENALTCLNDIEM
                     EFFNLFYNSKTKNNMTYISMKLHLDRTSCYNLKKKMIVKLSEIL"
     gene            complement(18635..18832)
                     /locus_tag="L3M60_001518"
     CDS             complement(18635..18832)
                     /locus_tag="L3M60_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226196.1"
                     /translation="MFKNNLKYYRKCKGMTQIQLARKAGITNDYISQIERGIKNPGLL
                     MAKKISSILEQNIEEVFFIQL"
     gene            19001..19321
                     /locus_tag="L3M60_001519"
     CDS             19001..19321
                     /locus_tag="L3M60_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HBN6226197.1"
                     /translation="MFAKRLRELRKEFGLTQRELGEKVGVSQRVLGYYETENRFPDEH
                     ILNKLADVFDVSVDYLLGRTLVKENIDTVAAHRKNPHEELPEEAQEQLNDYIEFLLNK
                     YKKK"
     gene            19403..19909
                     /locus_tag="L3M60_001520"
     CDS             19403..19909
                     /locus_tag="L3M60_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226198.1"
                     /translation="MNNLDKLFELASQEEIIIHYTTYIAGDLEGLYINKHGMKIISLL
                     SNLKQNTKKLTSILAEELGHHFTSLGYYVSSYNDYYTKIIIDKCENKALKWACEFLIT
                     EEDIIGIINSGVTCVYEMADILNVEINFFQKRLEFLSLKKQSLQLGNNKYLILTNLPY
                     FYIFDPIS"
     gene            20610..21365
                     /locus_tag="L3M60_001521"
     CDS             20610..21365
                     /locus_tag="L3M60_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN6226199.1"
                     /translation="MEKKVLATVGEKEITNIDIENALKSLDPYQAMQFKTEEGKKHLL
                     NDLVNQELFFLDAKEEKLDEEEIFKLEMKKIEENVLKQFAINKVLSSVNVTEDEKVKF
                     FEANKSSFSKPESATAKHILVDSDEKAKEILAQIKSEEISFEDAALKHSSCPSKDMGG
                     DLGTFGRGQMVPEFEEAVFSMAKGEVSEPVKTQFGYHIIKLEDLQESTESTFDEVKAE
                     VEKSLLYQKQNEVYGNKINALNAKYGNLVKYND"
     gene            complement(21508..21936)
                     /locus_tag="L3M60_001522"
     CDS             complement(21508..21936)
                     /locus_tag="L3M60_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HBN6226200.1"
                     /translation="MNILFFLTPKSEVAYIYEDYTIRQALEKMEYHKYSAIPIISKDG
                     KYVGTITEGDFLWTLKNDLNLDLKGLEDVPVTDINRKMDNSPVSINADIEDLVIKSLN
                     QNFIPVIDDQDTFIGIIKRRDVIGYCYEIIRGYKNLANDN"
     gene            complement(22357..22557)
                     /locus_tag="L3M60_001523"
     CDS             complement(22357..22557)
                     /locus_tag="L3M60_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBN6226201.1"
                     /translation="MKTGVVKWFNNEKGFGFISVEGEGDVFVHFSAITGDGYKTLEEG
                     QSVEFEVVDGAKGPQAANVVRL"
     gene            complement(22740..23909)
                     /locus_tag="L3M60_001524"
     CDS             complement(22740..23909)
                     /locus_tag="L3M60_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HBN6226202.1"
                     /translation="MGGVALKILKNKNKYIKGIMAFVFLSLATTAGVFLINDLSMKNM
                     EEKVMESANIEGDDVKDQYKLLLENLFDYRNKAILEKNENILKGLYETDKKFGLWAYE
                     NEVKKMKYLENWSSKQGVKFKDIKTRVKIKKIKEKEKDLYGIICSVSTEYKYSYENQK
                     DKENAFRIGTYHYLNVKIIDNQYVITKEWYTDPFADSLNLENIKSDDIRNYIGQQDGV
                     ELQLTDEQKKAIDYAHKYCGAAADEEHGMKFNPNYRDYNPEGGDCANFASQIMFESGR
                     FKKNSIWNYSERAGTKAWVNAQAFKNYALYSGRGSLIAKGSYEEVYKEAYNLRPGDFV
                     GYEKKGRITHVSTVTGLDSRGYPLVTCHNTDRMLVPWDLGWSDKSIRFHIIKINY"
BASE COUNT         7497 a         4214 c         2563 g         9792 t
ORIGIN      
        1 tctttgttct aatattttta tttcatttat tttctttttt ataattttat gataaaataa
       61 ttagtatatt ttgctttact tgaatgtaat tatttataca aaagtcaaac ataatataaa
      121 tgtagtaaat ttttagtttc aagtaatatc tcgttattat aactaaatat ttattaaaat
      181 taggaggata aaatgggctt aagagacaaa ttcgcgcaat catttgctag atcaaaaact
      241 atgagtggcc ctgagaaaaa ggctaatgaa ataatgggta aattgcttct taagaaagct
      301 atattaccta tcgttttaat gttcgtgata ataatcgctg gagctatgct taaaatcaat
      361 tcttgggtta cacttggcat aaatttagtt attgctgttg gtgctttctt ctacataaga
      421 aattctagta aaaagtatca gaactttaaa ccttatgttg gcaatttgat tagtttagag
      481 aaaaaaggta aaaaagagta cgttgctatt ataaaacaag gtaagttacc agttaagtta
      541 caaattgctt atggtggtga agaccttgaa catgttaaga aaaatcaaat ggttcagata
      601 agttataacc cagatgctaa aatagctatt ttggttaata gacaataatt taataaaaaa
      661 aggagttgtc ttaaaattga ttttttctaa tcaaatttaa gctcaactcc tttttgaatt
      721 tttatatatt atttttatta ctttttttaa ttaatatttt aaaatattaa atccttctca
      781 ataaaatagg gtactatcta caatactact ttatcatttc ccaacaatat ataatctggt
      841 gctactgcta cgttcatgtt atacaccgtt ttgtaacata ccttttatat cagtaacatc
      901 ttccttaatt tcctctacat ctgttttcat tattcccatt tgaacaagta tattcttatt
      961 tatttcttgt tgttgtgtag ataattctat aaaattttct acagtttttt tatacatgtc
     1021 tctatcttct tttttctctt gcatagtatt tttaaataat agagcacata gtatgccgat
     1081 tgcccctaga ctactcaatt cggttattaa tttatccatt atagcctcct attgtccaat
     1141 ttttttataa ttataacttt aatttttgtt acattcttac atcatgcaat attatatttt
     1201 caggattatc aatttaatct tttgtatttt aaattgtagg tgtaatttat agattgaata
     1261 aataataaat atatatacaa tttctaatat aacaaaaaat aagtacaagg cttacttatt
     1321 aaagttaaga ccttgtactt tacataaaaa aaataactaa tacatagtta ttctaaattt
     1381 actattcaac tggtatttct aattctttac atctaattct aacttgctct ctaattactg
     1441 ctggtacttc ttctattgtt tttttgcctc ttaaaattaa agctacatat acttctacca
     1501 ttccactctc acctccagat tttcttacac taaaagtgct tatattactt gcttcattga
     1561 cagaactttg acttaacaac atctcatata tttccgcatt tgctataaaa ttatttgtta
     1621 tatcatcatc ttgctttact tgacctatac ttatgtctga gatatcttta ttctgcattt
     1681 ctacttttct accttgtttg atttgttcta ttttattttt atcgaattta aacaattttc
     1741 acacctccat tttaagcttg tggctcgttg ttttcagcat catatctaaa aatcatagat
     1801 ggacgtttta tagctgtcca tttatctcct ttttctacta tgattttagg ctgagcactt
     1861 tttatgttgg cttcttctaa ttcatagatt tcttggtaac ttgagtctga ttttttgatt
     1921 tcaactagtt tcatgtcttt gaataagtca gatttattta tttttacttg aactttagtt
     1981 ttatttatta attgatatat agggtctcca agatttaaat cttcttcaag ccctctatct
     2041 agtgtaattg taatatcttg ttcattagat gttgtattaa ctaccttgta cattctatta
     2101 cctatcataa cataagaatc tttaattata gaatttccag tactctcttt aaaaataaat
     2161 aaagatgcat ataaatcatc acctttacaa taagctctaa atagtatctt attctctcct
     2221 attttaattt tatctggtgg tacttcaaat gatatttttt cagtagttaa atcacttaca
     2281 gattttacac tttcaccatt tataagcatt tctattctac ttatatcttt attagggtct
     2341 tgtattttta tcttaattga tgaaattgat ttttcagtaa tttctatagc agcatttgtc
     2401 gttcctactt ctgatataat atataatgta ttgtctgttg caagtacatc ctttactgta
     2461 tttacatctt cagaaatagg tactatagta aattctttta aaaattctac tggttgttca
     2521 attttatata catccctatt ttttccagta acccatactg aattatcatt tttaagcacc
     2581 aatatatggt cacttccata agattttatt tctcttatgt ctgttaatgc ttttgtaaag
     2641 aattttataa tagcattttc attatcgctt cctaacccta aagctccctt atcattatat
     2701 ccttgtacaa ataattcatt acctgtagtt actactaatg tatagttttt accacaatgt
     2761 acttctttac aactagatac agttgtaatt ctttgtaatt cagtaactgg attattgtga
     2821 cctataccta attggtattc agagttttta ccaatagacc aaagactatc atcagatttt
     2881 ataaagtaag taaagttatc tccacatgaa acatgcttta catctgatac attaatctgc
     2941 acaaattcat ctacttgatt tatagataaa tcttgcaata attctccatg gtagtttgta
     3001 ccacatccat acattgttcc atcattaagt aatataataa tatgagtctt agaaatagat
     3061 acttctttta tattttcata tggaagttca gtataaaggt ctcctttgta caaatttcct
     3121 aatactaaac cataatctat gtcttttgtg gtaggtataa ctatcttatt atttagaagt
     3181 ataaagttat gtctgttttt aatagatgtc aaatctccca ttacaaagtt catatgctta
     3241 taattaaata ttctattatc attatttcta ctatcgtatc tatttccaac acctaattgt
     3301 ccatagttat ttaatccaca aatatatata tcattagtat cttttattgc tatatttgca
     3361 tattctaaag gaagtatttt ctttactctt gtattggttt ttataaatgt atctcttttt
     3421 gttacatcac caacacctaa ttgcccacaa tcattaaaac ctgtaccata taagtctcca
     3481 ttatcgctta cataatgtcc acatctataa ttcattccaa tgtcctttgc ctttgatgct
     3541 atttttttaa tcttctctgg agtctcactt cctgtacctg aaccattact acatttacct
     3601 gcagtatata catccccttc acttgtaagt aaaagtactg cattatttct gtttggagct
     3661 acttttacta catttgaaat tggaatttca gaaaatgaac tttgattact gttactattt
     3721 agacctaaac aataatcagt atttaaacct gtactatatg cagtattata gattgttatt
     3781 atataagtat ttatttcatt tggtataact tcttttacat tttccataca ctttataaaa
     3841 ctgtactggt cagtagtatt tcccatacct aattgtgaat tattattgta tccacaacaa
     3901 tatgcaatgt ttgtatcctt tacaatgaat gttgttgtat ctgagcagta cacatctctt
     3961 acattagtta tattgtttac agtaaatgtc aatacgtctg gatggttgct ttcgcttcct
     4021 aagcccagtt gcccatgagt gttttctcca catgaatata atgtgttatc atctttgatt
     4081 gcatgtgagt gggtagtccc taaaacaaca tctcttacat tttgtatatt tactttggta
     4141 aatgtgtttc tatttactat atcacctaat cccagttgtc ctttggtatt taatcctgtg
     4201 gagtatagtg ctccatcatt tctaattgca aatgttgtgt taccatctat acttgttgat
     4261 attttcttta tattatcaag gtttatctga gtgaacttat ttctatttat tatgtcacct
     4321 aatcccagtt gtccagaact attatttcct gtggcatagg ctgttccatc attttttact
     4381 ataaacattg tatttcttga tgttgtaatt tcttttatat catccataaa tataaatgag
     4441 tttgattgtt cagttatatt tgtgaatttt cttattggtt gattgttata ttctcctata
     4501 cctagtttgt taaattgatt tgttcctgtt acatatatat tgttgttttt atctactact
     4561 actacagtgt tattttttac aattatatct tttatgtctt ttaatcctgt atcataacta
     4621 cttacaccat ctagatttgt tatatttcca aattctccat tattagcaac tccatgtcca
     4681 tatagtttag aactgctaga agttataata aaaagacttc ccaaatttga atattcagaa
     4741 ctaatagtag tgtaactttt atatccagta gcagaagaac caaatgtttt tggatatcca
     4801 gtaacatatg tactaccttt tttaaagtat aatatattat ttgatgttcc tattactttt
     4861 tctattccat taaaagcgtt tacttgtgta aagtaagtat tataataaga actcgtagtt
     4921 tcaataaaac ttccatatct tgaatagtca tttatacttg gattgctctt tgatgtatct
     4981 ggatatctat gctgtcctgc taaataaaca aatccacttt tgtcaattat tgcacaacca
     5041 taattaaaag gaaaaacatt aactatttta ggtaaatcac ttagattgct agaactagta
     5101 gacaatgtaa ctgttctata cttagatact aatgttttat ttgtgctact actattgcct
     5161 gtacctaatt gatagtaact attatatcca gatacatata attttccttc actatttatc
     5221 atatatacag aacgataata tgttgctatc ttttctatac catttatatt ttgcttatct
     5281 gggtaattag aagttctttc tatattacta ttagcactat aaccccaccc atatacttct
     5341 tttgaatgat ttagtgctat aatatattca ctttctacac aaaactcttt tacattctct
     5401 atttctattt ttgtaaatat atttctatta acttcatctt ttaaccctaa tatcccataa
     5461 ttatttaaac cacatgaata taatgttcca ttattcttaa gtacaaaagt tgagtttcca
     5521 tttcccttta tatccaaaac attatctata tccaccttag tcataactga cctgttaaca
     5581 gtatctccca gtcccaactg tccataatta tttaaaccac aagcatataa cgtaccatca
     5641 ttcataacaa taaacatatt attatctgtt aactctatct tcttataatc ctgaaaatta
     5701 tcacttaact ttacatttaa aaattcacta ttatcctttg taacatacaa ataatcatca
     5761 ttaaccaata aacctgaata attatctaaa tttgaagatt gtacctttga aaactcatac
     5821 agaaaagttg tatgaaaaaa tgaaccaagt tgacctgcca gattacagcc agatacaaat
     5881 atttcttttg aaccatttat taaaaaatta aattgagaac cacatttaat atcttttata
     5941 ttttctacat taaccttaac aaaagtattt ctggatgcta catctttatt ggcattggct
     6001 agttgaccat aattgttttg tcctgtacta aatagtgtat tatcgtattt taatatcata
     6061 gtatgattat ttccacaaga agcatactta acattattta tactagaaag agtaaataca
     6121 attgaattat tattatttcc tgtaccaagc tgaccaactc cattatagcc tgtagtatac
     6181 atagttccat cattcttaat aagaattgta tgactactac cacacgctat ctgttttaca
     6241 ttgtccatac cttctatttt attaaaaaca cttttattta aagtactatt aattcccaac
     6301 tgcccatttg aatttaatcc acatgcatgt gctgtcccat ccatttttat gataaataca
     6361 gattggttat aagttattac atcctttaca ctatctatat ttactttagt gaaagtagct
     6421 ctattgttag tatcacctaa tccaagttga ccacttgagt ttaaacctgc tgaatataat
     6481 gttccatcat ttttaagtac aaaagtaaag tcataaccac atgcaacagt ttttacatca
     6541 tatattggta gcttagtaaa ttcataatat actgtagact caattacacc taattgacct
     6601 acattgcttg ttcctgctcc atatgcaaat ccatctttag ttactgctac actgtgtttt
     6661 gaaccacaag atacatattt tacattatca atttttactt tactaaacat ccttctctct
     6721 gacgaatctt ttccaagtcc tagttgaaaa cctgtattat cgccacaagc atataaatta
     6781 ccatctgatt ttaaaccaaa agtactatta cttataccag taaaaaattc cacaaaatca
     6841 taatcattat atcttttatc aaaatcatca atcaatatgt tcttatcatt aaaataagca
     6901 ccacgttgta aaagtttatt ttgcttcatt attagtacta cctcctttat ttatcttatt
     6961 tcatatgaat atttacttag agttatatct ttttgttctt ttaaataagt attactagat
     7021 tttgtctcaa caaagttatt atccatatca tgtatatatc taactctatt tgcactaaat
     7081 ggtaatacta catctctaaa aatcacatta ccaatttcac ctgttatgca ttttctattt
     7141 aaatctaact taagcccttc tatagctact atgttatttc catttgttaa tgtatcatac
     7201 caataaccag cctcatctac agttacctct gattctaatt ctaggtatat taatatatct
     7261 aacacttttt cagataattt gttatagcta ttttttaata ctgatatatc agtttgtatt
     7321 tgaccactta taacatcatc aaaagatatg ttttctattt ttttaactac ctcatttatg
     7381 gctcctgata aatctttgga atctgtagtt aatatattta catcaccaat gtttactttt
     7441 atttctttta tatttttatc gaacttatca tctaaatctt gaatatcctt ttgagttaca
     7501 aaaaccaatg tcgggtctac ttctaatgta acatttgaag tattggacaa ttgtaaaatc
     7561 atttttacaa ccaaatcttt aatactgcca tcttcagcac gtggcttata cgtctctgga
     7621 tacttaccta tagctattat attattctca ttatccagaa cagcagcttc tcttatcata
     7681 aatccaccaa ctgttcctgg taaaatagtc tctataacta tccaattagg atttttttca
     7741 tcaattctta gagaatttat atttccttcc caaactacat tctttaaagc agtttgttcc
     7801 tctgttggct cataatattc tccattacta tctccaactt gaaacttggt tagtccaaca
     7861 agttctccta gtgcagttgc atttgctatt ttttctctac caattttggt taatattata
     7921 taaaattttt cttccaatta taccacctcc gatttaggat aaactactac attttctaca
     7981 cctgtatcat ttgcaatagc tatattaatc ttacaattat tttcaataca tttaatgctt
     8041 aaaggataaa cctttatttc ttctctactt aacattccag ctccacagtt tacaacagca
     8101 ctgcatttta taattggttc aaagtatgga tgaactttta cctcttcact acataccata
     8161 cctccaccac aatagactat acttttatct agtattattg gttctaaata gtgtaataag
     8221 tgagcaggtt ttactctttc aatcatatcg ctacaatcta aaagagtttt atagtcacag
     8281 ttatttgcta taaaactcaa tacgaatgta aattcatcac tataaacttt tatatctgtt
     8341 tctgattttg tatatgcctc acatatactt tttataactt caatagtact agtacctctg
     8401 cttctcattt tagcttttat attgctacgt cttgtgtcaa aatcaagttc ttttttttca
     8461 atgcagagta tatcttccca catatctaat ccccatgtcg ctgtatcaac aaataattgg
     8521 ttgaaggtat catcaatatc ttctttaaga atatttaatt ctgtatcgta tgcaccttgt
     8581 atctcttcta caatgtaatt tctatcaaat gatggtagtt tatcaatcag cttcattgat
     8641 tttccacctc actaaaatta acagttgtta cactaggtat tttatcttca ttaatagtta
     8701 tattgtctgt acttccattt ataagtaggt tgcttaaatc atgtacacca gcagtattta
     8761 taagtattcc catgactttt gtatagatta tctctcctct aatatctcta aagtatgtat
     8821 ttatactttc taggaaagat tctttgacat tatccaatgt atatccatct tctagtttca
     8881 ttactgcact tatatttatt tctattggta atggcgtcac aactgttata gttggtccaa
     8941 taggcttctc ttcatctata tgttgttgac atctttcaat cgtttctgta tcaacagctt
     9001 gattattttt cccaaatatc aagactttaa ctgttcctgg accatcccat cttggataaa
     9061 ccttaacatt atagactcca tctacttcca aagcccattc ttcataatga gctttatttc
     9121 cacttgtagc ttgatttctt tggattttat aaaatctttc ctttagctct tcatctgtct
     9181 ctatatctgt accaccttca aaacttcttg tgttagtaat ctttgttact ccagatatat
     9241 tatcaactag tttaaattca caatttgtag gtaaattata tctcttacca acttccagag
     9301 cttgaactgg actattgtca ccttcctcac taccaatagt tacatctttt attactacaa
     9361 agagtaaatc tctatatgat attactgtgc cattatttat tacagttcca ttctctccaa
     9421 taaattcaac ttctccattt gactctgtac ccaactttct atatacacca aattcattga
     9481 ctcttttatc aagaaactgg ttatatgtgt cttgtataaa agccatttta tgtatctttg
     9541 aaagttctag atatatcttt gaaagttcta gattgtttcc agatatcatg ttgtttagaa
     9601 aagaaccttc tcctttataa atatcaagat taatattttc aagagttcta ttttttatta
     9661 cttcatatgt ctggtcacta tacatttatt tccacctccc catagatagt ggatactttt
     9721 acatttgcac tcaatatgtc tctgttaaag ttagcacttc ttacacttac ttctaatata
     9781 tatggattta ttagaagggc ctcttttatg aatctactag cttcactttc tgtaagacct
     9841 tttgtatatt tttgtcctat tagttctgaa agctctgtcc catattctaa agagtatatc
     9901 tcatgttcat atctatttgt ctttatacac ttgtatatcc aaacttttaa ggcttctttt
     9961 ttctcaatta ttttaaaatc acctttttct aaaataggtt catctttttc aaaatcccat
    10021 gccacttcac gaaagattgg caattcttct gttttaggta agatataatc ctctgggaca
    10081 cctataaaag gaaatattgt actcattata agctcaccaa cttacttaca atagcaaatt
    10141 tttcgcctat tctaaacatt attaccatat ccccagattc aaaagtatct ataaatggat
    10201 tttttacttc atgtttgtgt tcttgatttg tttctgtatc aaatgtttct atctgtctat
    10261 caagcatcca actatctatc aaaatatctt ctttttctaa tatgatgtta tttgtctcta
    10321 tctttaaatc tggtaattta cttttaattt ttccaataaa aaaagaaggt tgattatgat
    10381 gctttccttc ttctcttatt attcctataa attcatttat tggattagcc attatatcac
    10441 catcctttta taatactctc cttgctgtat taaaatcttt tctactactt aacttactta
    10501 ttttaactac atcacctttt tgtggagcat gtaaaaattc tccattacct atatatagtc
    10561 caacatggct gactggatta tgaaaaaata ctaaatctcc tgcttgaaga tttttttgtt
    10621 ctactttctt gcctttttta gattgttgat ttgatgttct tggcaaacta acattaactt
    10681 ttttaaaaca atagaccatt aaaccagaac agtcaaaact acttggtcca tttccacccc
    10741 atttatatgg tttacctaga tgtttttttg cttctgatat tactatttta gctttatcag
    10801 atacattacc agaataacca ccgataataa tttttccttt tcttcttcca aatttactag
    10861 cttcttcaac atttccaaat aaaatatcta tgtggtatgt tccattcttt tctatagtta
    10921 ttgcaggtcc tacgtcattt actttataga ctccatcacg accagaaact cctgtaatta
    10981 cctgtatttc atctccatag tttaatagag gatgtttatt taaaaactct tttgtataat
    11041 aagtttgctc atatttacca accataggag cagcgcaagt ttttttagat gggtcaagtt
    11101 ttttctttct acaatctgta tctccacctt cttcttttct aggacagtaa gctgtaaatt
    11161 ctgctgtaaa ctcttttcct cctgcataac cttctcctcc taaattactt tcttccttag
    11221 gttcatcctg tcctgctgac ttttcatcca taagattttg aaaattaagt tcaagttcaa
    11281 tttgatactc tccattttgc caggtatgtt tgtctgtatc tatataaaat agtcctacaa
    11341 gctttgtata agaatctttg acttttaccc ctctaccagt tatacaactt acatctccat
    11401 aacctttaag agaacagctt ttttctatcc cattaaactc gctatcaata tctacatctt
    11461 gattttcttg ttgctgaatt actttttgca ttattacatt tacttcctta aaaatttcat
    11521 tatctatttt ttcgctaatc ttacttccat actggtctac cactattact ttatttttta
    11581 cattttccat actctccgaa aaggtggtat ttataatatt aaatccctct tcaaacataa
    11641 cacttaatgt aacagttcct ttttcaataa cattaaactt atctaaattt gcctctatca
    11701 tatacttttt ctttgtcttt ttacttgcct ctgtatatgc actcattatt gtgtcataac
    11761 catttactcc tataaacatc tttgtatact tgacattggt ctttgctatt gttccaactg
    11821 aaatcctatt ttcagcaaat acttgctttg ctatgtcttc aactaactta tctttaaaat
    11881 tgtatgacac ttcactttgc ataagtagaa atcccatatc tttagataca aaactaattt
    11941 cattactgct tgaatcttta gacctattaa ttatcattcc tctaaaaagt tctttatcat
    12001 ctacgtagaa acaaactgta ctagctattg gtatatctat ctgttggaaa tttacatcaa
    12061 atgatgattg tattattgaa aactctagtg tccttgatgg agatttataa tcacctgacc
    12121 atgatacctt gtctactatg tcagttatgt catatatact tccgtttttt atgtgtaccc
    12181 aaatcttaat aataaaaaat cacctgcctt ttcatttaat tctatcagca gttatttata
    12241 acttattatc tataatagtt attgttttgg agttactttt ggtattttta ttcttttata
    12301 ctcttttaag tctaatgtaa aatacacatc tcgagtccca tctcgttctt catagctaaa
    12361 gccctcaatt atgacctcca tatttatatt tgtttctgta attgtaaatc ttagtataaa
    12421 accttccttc atccattttt caatcttatt tacacaatca tatggtgatg gaaaacctgt
    12481 atagtcacag aaagtatagt ctttatttgg aaaaaaactt gatattgaaa tgcttttaag
    12541 agctacacca ccaaaagttg ctatttcacc tattttgagt atactagaag agtttacagt
    12601 agcttttcca ttaatactaa aggaagatgg aaatactgga aatctaaatc tatcttcggc
    12661 ttgtctaagc cacatttcca ttataataca cctcccaatt gtgccatttt tatacttgaa
    12721 actaattttg atgtaacttt ttctatatca gcttcttctc taatcactat tgtatcagct
    12781 aacttagcta tgtttatatt tccaccattt tttctgcttc tatattgatt tgcttcttgt
    12841 ttagttaaaa ccatttcacc ttcatggagt ctggtttgat agtcattata aggaacatag
    12901 ttcaatccaa atgcattatt acctttctta cttgaataag ctttaccaga atcaccagcc
    12961 tttgttccag catatggatt atctttgtaa ttttttttac tcttttccac tagatttaaa
    13021 actgttcctg tgatgcttcc attttttatc ttattaatca tagtttgcca ccatttgcat
    13081 atatctttaa ctattttaca tactttatct aatatgttaa aaaaagctcc caatattggc
    13141 tctataattt tccatccagt ttccaacaga ggtcctaagg tctcccatac agttttccaa
    13201 ataacaccca gtgtctctat aaaattgttt atttgaggtg catattcacc taaaaaagta
    13261 aaaattcttg tgataatagg actaacagct tcaactacag gcccgattaa ttggaatgct
    13321 gtagttacta ttgtaccaaa tatacttatt aatccttgtg caattggtga tttattgttt
    13381 atactatcta gtaaattatt tactaagttt actactggaa gtaatgaatc tattaatgct
    13441 gtaaaatcga tattttgtaa tagtgtactt cctgcgttaa caattgcttt tggtgcattc
    13501 cctatcattg ttgctgcatg attttcaaaa cgttcttgct tattttcaac catcatttta
    13561 cgcttttcag cctcattaga aattcgatat tgagtagaat cagaaaactc ttttccattt
    13621 tttacacttc tagattgcat ctggtcatat ttgttgccaa taattatctt accattcatt
    13681 acattgccta ttttaccagc cattttttca gataagccag ctaaattgcc taatccactt
    13741 tctataaaag tttttatttt ctttagtcct tgacctaaaa tatcatcaaa aggtttaaca
    13801 atactcttca aaccattgtt aatgctcgtt tgagctcctg atattatacc tggtattgta
    13861 ttactcattt ctttagctaa tcccatctgc tttacactta taccttccaa tgcttcatca
    13921 aaagttttat aattttctcc taccagagta ttaagagatt ccatatttcc ttttgctgca
    13981 cttaagaaag ctgctgatac atcttgttca ctacttgtat tcatatttaa agctctaaca
    14041 tctaccatag cctgagttat atctttttct tttttaccac cagtaatatt actaatcatt
    14101 ttagctttag ttcccattgc aacaacatct tcagggtcaa aaggtgtctt atctgctata
    14161 tctaacaaat cttcataata attatcaaca tctctttgat taacttttcc agtatttctt
    14221 ttcattacac tttctagaac aactttctga ttttgttcct gaccaagtaa gtcaacaccc
    14281 tttttcacag cgacacctat agtagcacca tttgtcaaat cagatatctt cccttttatt
    14341 gaaccaagta ttccacttgc catatccttt acagctattg cacctctcca taccttacct
    14401 gcaaacaatc tcaattttgg aaatacttta cttataatgc tacttgccat atcttttata
    14461 gctattacac cactccaaaa agtattttta aacaacatta acttaggcaa aattctaccc
    14521 atagcactgc ttaccatatc tttaagatta atacttcctt gataaggacg tttctctact
    14581 cgttttaaat ttcccaagat actacttatc actctcttag tcatatcttt tgcttttatt
    14641 acaatcttcc aaaccttgcc accaatcttt tttaaatttc caagtaattt atttttagtt
    14701 ttttctattt catcactttt agctttaaat attaatataa tcataatatt tgaatttaac
    14761 tcatttttct tttgaatcaa cttattaaaa taatcaataa tcatagctat agttattgct
    14821 agtctcataa tacttccatt taaacttcca ataactcctg aaacagcatt tatgctagaa
    14881 cttaattttg tcatagttga acttacactg ctagcggtag tctgaacttt tcttaacgaa
    14941 gaatttaaac tgtttactct actacttgca cttccaatag aattttttat gctactagcc
    15001 atcacattag tagaaagtga aacagccaca gttgctctcc taaaattatc agttactgat
    15061 ataatttgat tcatttttgg tgtgtaatta tctgtaagat acatatttat accttcttct
    15121 cttacatttc ccattacata ccacctcctg tcaaaggaca cactccatat ccatgtttca
    15181 ttttttcaac ttcttcctca atttctaagg cgaaaaaagc ttcaataagc tttaattcgc
    15241 ctttatccat tgcataaaaa agggacggcc ttataccttt tttcttccaa taatagtaca
    15301 ttattgtagt cttggtatcc gtccctatta gttttttacc tcttctatca ctgcattttt
    15361 accataaccc ataacatctt gaagtattct atataagttg gtaatttcac ctggtagtaa
    15421 taattttctt gccaattctt ttggtgttgg agccttaaat ttatccatta gctccttatt
    15481 tttaaataaa agctttccat ctaaatcaca aacaccttct ataattgtaa gcatctgcat
    15541 ctccggtaag tctatatccg ttttcatatt ttcatcaatt ttaagacaag tattttgtat
    15601 ctcttcatct ttacttattg ttaaagctct acacattact ttaaattcct gcccaaatat
    15661 agttgataat ctcttaagtt caactatagt gctcggtctt tctaagtttt ctgtatctga
    15721 atttaaaagt aaatctacta tatttatatt ttttgataat ccattttcac tcattattat
    15781 ttgctccttt atcaatcaaa ttcaatcaac taactttttt acttattaac tatctgaatt
    15841 tattaagtat atacttacta aatatcatta taaaaccaaa tataaaacta aattatatca
    15901 agaaaatcat actcagtaaa tgtgaaaggt gcttctatct ctccttttac tccaacctcc
    15961 caatcagcta aagttaaatc atcaaacgct atatttttta ctgctattct ttctgctcca
    16021 taagaatctg ggtcatttaa tttagatata gcaacaaatc taggttcaga acctctcttt
    16081 atcttttctc caattaactt atgcattctt gaactaacat ggtatagagt tattgaacct
    16141 tttcctttat atcccatata ctttgtatca gtacccattt gacctgctat tataatatcc
    16201 tcttttgtaa attccatctt tgcttgaaac ttctttactt ctgctacttt gtttccatca
    16261 agccaaagtt ctccccaagt accactcatt acatttctag cttccatatt tgccatatta
    16321 ataatcctcc tatatttcta ttgataaatc tatatcttcc atagcatcaa gtacttttat
    16381 ttttgctttt aaaaatactt tagaacctgt gttagcttct tttatttctt gtaatgtcat
    16441 ataagataaa tctactcctt ttgattttaa atacgatttt tgtgcttcaa aatctatttc
    16501 aacagtagaa tcagattcta taagtgcaga tttttctaat tcttctaaat aactttttat
    16561 agctactatc aataaacatt tgttgtcata actgttagta acctttccta tatagtcatc
    16621 tattatcacc tttcttatat cactatgtat aatatctaaa gtgtcaacta tttttatttt
    16681 ctggaacatt tctccttttt ctgctgttaa ttcagttaaa gaatttactc ctctagcaat
    16741 tcttatagct cctgcttctt taataagtat aagctctcct ttattaaccc ttgattctgc
    16801 atcaacttta gtcatcttag gtatatcgac tacatcacta agcttagtat aggttactga
    16861 ttgacttaaa ggtgtacctg ctataagccc agctacccta cttgtaaact catcaacact
    16921 gtatttcttt tctccaacta acacatcttc tgtagtaaaa ttaattatcc cttcatgatt
    16981 tcctacaact tttcctaata tagctttaac cttaacctta tctatatctc taagtttaat
    17041 tatccaattt tttatagcag tcttatcagc ttctactgct tttggcatac atagataatt
    17101 aaattcctta gtctctagga agtctaatgc agtttgaata tctgcttctc cttctattac
    17161 ataaactaat aacttatttg gagtgttaac atttcccatc aaagctaaat ttatatattc
    17221 tttattttca gcagataaat caactggtat atcctctttt tcatgtatct cgttaagacc
    17281 taatgcctta gcatctttaa gcaccattgc aattattcct ctagctgaac gttctttaac
    17341 agttgtagct agctccttaa atgatatgtt gatacttggt aatcctatag ccatactatc
    17401 actctcctaa atttaaattg atttcttcca tattttcata tactacatct tcttcatata
    17461 cactatccag atagtttagt gtaaccgaaa aattcaatgc ataaattgac tcttcttttt
    17521 taatactttg ttctagcttt gttatattta attttctgtc tgttaccttt atatttctgt
    17581 taaatagctt atttaattca tctgaaattt catacattct aatactcttt gactgtggtt
    17641 ttggtaagta ttttatagag ataacaatat ctttgttatt cgtatttaac attataggtg
    17701 tacaaataac tgataatata cttacaaaaa aacaagactc ttcagagcct tgcacacttt
    17761 catcatcaat aaaaatactt tcagtaaagt tttttttgag aatctcaata attgtttcta
    17821 atatttcctt gtatttaagc aaataatcac cacactatca ctaaatattc ttgcttaatc
    17881 tgataatctt actataaatg atataagtca tttttaaata tacatctcat ttattcctgt
    17941 caagtatctt ctatatttct aggtaatatt attcttttca atctatcacc tccttcaata
    18001 tggatttctt tcacaataac attatctcac atattttatt acctattgtt gcaatgttgt
    18061 atataaagtg ttgtaaaatt gtcctatttt ttataatatc tcactcaatt taactatcat
    18121 ctttttcttt aagttgtagc aggatgttct atctaagtgt agtttcatag aaatatacgt
    18181 catattgttt tttgtcttac tattataaaa aagattaaaa aattccattt ctatatcatt
    18241 tagacatgtt agagcatttt ctatcttctc tttttctatt tctatatctt ctttttctat
    18301 ttttaatttt cttaatcgtt cctccctttt caacacctca ttttctacag aggaacttat
    18361 attatatgtg ttactagtct tttctgtata taccattgct ccacatccag agtattcatt
    18421 ttcatatctt tttatatcta aattaatgtt ctttatcttt atatccaaat acttgtaatt
    18481 atgtaatctt tcatctgttt ctttaaataa tatatctttt ttgttttcca tagtatttct
    18541 cctttttata tatttattgt tagcttcaaa tttacaacaa tctttttagt actaattttt
    18601 ttattaatct taataatttt aagaacattt tgttctataa ctgtataaaa aaaacttctt
    18661 ctatattttg ttctaaaatg ctagaaatct tcttggccat aagaagccca ggatttttta
    18721 tacctctttc tatttgagat atataatcat ttgtaattcc agcctttcta gcaagttgaa
    18781 tttgtgtcat tcctttgcat tttctataat atttcaaatt atttttaaac attgttttat
    18841 acctccatta ctattaagaa tattttgttc tcctatattt agattatata gaattatttg
    18901 ttctttatca atagatttta gaaaaaaata ttcttttata ctttactagt tttatttttt
    18961 agaacaattt gttataatct atacataagg tggtgttata atgtttgcca aaagattaag
    19021 agaacttaga aaggaatttg gattgactca aagagaactt ggagaaaaag taggtgtttc
    19081 ccaaagagtc ttaggatact atgaaacaga aaacagattt cctgatgaac acattttaaa
    19141 taaattggct gatgtatttg acgtatcagt tgattacctt cttggaagaa ccctagttaa
    19201 ggaaaatatt gatacagtag ctgcacatag aaaaaatcca catgaagagt tacctgaaga
    19261 agcacaagaa caacttaatg attatataga atttttacta aataaatata aaaaaaaata
    19321 aaccatatga tgagcagtct atttttctgc tcttttaata taactcaaag gcaaacatac
    19381 attctatttt agggggattt ttatgaataa tttggataag ctttttgaat tagcttctca
    19441 agaagagata ataattcatt ataccactta tattgcaggt gatttagaag gactatatat
    19501 aaataagcac ggtatgaaaa tcatatcatt actcagtaat ttaaaacaaa atacaaaaaa
    19561 attgacatcc atcttagctg aagaacttgg acatcatttt actagtcttg gatactatgt
    19621 atcctcttat aatgattatt atacaaaaat cattatagat aagtgtgaaa ataaagcctt
    19681 aaaatgggct tgtgagttct taattactga agaggatata ataggcataa ttaattctgg
    19741 cgtcacttgc gtttatgaaa tggcagatat acttaatgtg gaaattaatt tttttcaaaa
    19801 aagattagaa tttttatcat taaaaaaaca gtctttacaa cttggaaata ataaatattt
    19861 aatattaact aatttgccgt atttctacat atttgatcca atttcataat aaaaatagta
    19921 gtaattttaa ctggcttacc agtaaaatct atatatttat tttaacaaat tttggtttct
    19981 ataaattcaa tattaatata taataatgat atcatattat ttaaaattct aaaaacaaat
    20041 atatgctttt gttaagaaag gttgtttgat gaatgtataa agaaaagttg tttgataatt
    20101 attttaagtt cttagcatta tttttttggc caataatgtg gtataagtgg atagtaattt
    20161 caaatggtac tcttgaaaat atgttattta ccacctacgc aattattgca attgttttca
    20221 tcattttata ttcagtatct atgataaaat ataaagacat tacacaaata gatttctttt
    20281 acaggataag tactctactt gcttttatat ttacactttt tagcttctta atatacccaa
    20341 agagtttatt tttcttgtat ttaaagatta tttttactgg aatatacctt tattattcaa
    20401 tagtaaaaac acttaaattt aaagatgacg agggtgtagt gggtattatg agctcacttt
    20461 tattaattgt aataacttta ttctactaaa tttaattcta tttgacttaa tatggcttta
    20521 aatattagac atctacattg ttttctgata ttatttaaga tggtagtttt atattaataa
    20581 tgaaatacac tatgaaggag ttaactatta tggaaaaaaa agttttagca acagttggag
    20641 aaaaagagat aactaatatt gacattgaaa atgcattaaa atcattagac ccttatcaag
    20701 ctatgcaatt taaaactgaa gaaggaaaaa aacacttact aaatgattta gtaaatcaag
    20761 aattattctt cttagatgct aaggaagaaa aattagatga agaagaaata tttaagttag
    20821 aaatgaaaaa gatagaagaa aatgtattaa agcaatttgc tataaataaa gttttatcta
    20881 gtgttaatgt aacagaagat gaaaaagtaa aattctttga agctaataag agttctttct
    20941 ctaaaccaga aagtgcaact gctaaacata tattagtaga tagtgatgaa aaagctaaag
    21001 aaatacttgc tcaaataaaa tcagaagaaa tatcttttga agatgcagct cttaaacatt
    21061 cttcttgccc atctaaagat atgggtggtg atttaggaac attcggtaga ggacaaatgg
    21121 ttcctgaatt tgaagaagct gtattctcta tggctaaagg tgaagtaagt gagccagtta
    21181 aaactcaatt tggatatcat ataataaaat tagaagacct tcaagaaagt acagagtcta
    21241 catttgatga agttaaggct gaggttgaaa aaagtttatt atatcaaaaa caaaatgaag
    21301 tttatggaaa taagataaac gctcttaatg ctaagtatgg aaaccttgta aaatacaacg
    21361 attaatcata aagtaatgta taaaaaagct gtcttttcac aatatttatt ataaaagaca
    21421 gctttttata ttaaagttta aatatccagc ttaatattat cattctttta ttgcaaagaa
    21481 ctcagcttaa aagtttaaat aagattttta attatcatta gctaaatttt tatatccacg
    21541 tattatctca taacaataac ctattacatc tcttcttttt attatcccaa taaatgtgtc
    21601 ttggtcatct ataacaggaa tgaagttttg atttaaagat tttataacta aatcttcaat
    21661 atctgcgttg atggaaacag gagaattatc catttttcta ttaatatctg tcactggaac
    21721 atcttccaat ccctttaagt ccagatttaa atcgtttttt aatgtccata aaaaatcacc
    21781 ttctgttata gttccaacat atttaccatc cttacttatt attggaattg cagagtattt
    21841 gtgatattcc attttttcta gagcttgcct tatagtgtag tcttcatata tatatgctac
    21901 ttcacttttt ggtgttagaa agaataatat gttcattaca ctacccaccg ttactgaaaa
    21961 cttataaata ttattcaata tttataatgt tacttttttg cagtaacttt ctcctttctt
    22021 attttctaag tattcttaat actatacgct gtaacaagcg tttttctttt ggtcatttta
    22081 atcttattat gctttattaa tatttttata tggtctatca gtatcactta ttctcttttt
    22141 agaaaattca ctaaaaatta attgacttaa acttagccat aataatttta ctctacacta
    22201 ttaattttaa catatttatc tcattattaa tgattttatt ttaataattt taatttattt
    22261 tgatattaat tttatttaca aaaactgaaa aaaccgctaa atgattagcg gcttatctct
    22321 catattacta gaacactaat atatatgaat ttttaattat aatctaacta cgttagcagc
    22381 ttgaggacct ttagctccat ctactacttc gaactcaacg ctttgtcctt cttctaatgt
    22441 tttgtatcca tctccagtta tagctgagaa atgtacgaaa acatctcctt caccttctac
    22501 agatatgaat ccaaatcctt tttcgttgtt aaaccattta actacaccag ttttcattaa
    22561 taaatcctcc taaaaaaaca caaatattta gctactcttt tctgtaacct tattactact
    22621 ctatcatata tagctatgtt tgtcaattat tattattttt atacattaat aatcaatttt
    22681 tttactttat aattttaaat cattattatc ttttacactt tatttttctt tatatttttt
    22741 caataattta tttttattat atggaatctt atggacttat cactccaacc taaatcccat
    22801 ggcaccaaca ttctatccgt attatggcaa gttaccaaag gatatcctct tgagtctaat
    22861 cctgtcactg tcgaaacatg tgttattctg cctttcttct cataaccaac aaaatctcct
    22921 ggacgtaaat tataagcttc tttgtatact tcttcataag aacctttagc aattaatgaa
    22981 cctcttccac tatacagtgc ataattttta aaagcttgtg catttaccca tgcttttgta
    23041 cctgctcttt cactataatt ccaaattgag ttctttttga accttccact ttcaaacatt
    23101 atctgtgatg caaagtttgc acaatctcca ccttctggat tataatccct atagtttgga
    23161 ttaaatttca taccatgttc ttcatcagct gcggctccac agtatttatg tgcataatct
    23221 attgcctttt tttgctcatc tgtaagctgt aactctacac catcttgttg accaatatag
    23281 tttcttatat catcagattt tatattttct aaatttaaag agtctgcaaa agggtctgta
    23341 taccactctt tagttatgac atattgatta tctataattt ttacatttag ataatgatat
    23401 gtacctattc taaaggcatt ttctttgtcc ttttgatttt catatgaata cttatattca
    23461 gtcgatactg aacagattat accatagagg tctttttctt tttcctttat ttttttaatc
    23521 ttaacccttg tctttatatc tttaaatttt acgccttgct tactagacca gttttctaaa
    23581 tacttcatct tttttacttc attttcataa gcccaaagac caaatttttt atctgtctca
    23641 taaagacctt ttaaaatatt ttcatttttc tctaatattg ccttatttct gtagtcaaat
    23701 aagttttcaa gcaatagttt atattggtct tttacatcat caccttcaat atttgcagat
    23761 tccatgactt tttcttccat atttttcata cttaaatcat tgattaaaaa tacacctgct
    23821 gtagtagcta aagataaaaa tacaaatgcc attatacctt tgatgtactt attcttattt
    23881 ttcagtattt tcaaagctac acctcccaaa tagtacacct tacaaatcaa attacaaaga
    23941 atattctgtc acatacaata attttcatac ttaataattc tatattttat tatgtccaat
    24001 attaaaaatt aattgtatca atacactgtt ttttttacaa ttttttattt tataatctac
    24061 tttttt
//
LOCUS       DAFNNB010000043        23796 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0043, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000043 DAFNNB010000000
VERSION     DAFNNB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0043"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(552..824)
                     /locus_tag="L3M60_001525"
     CDS             complement(552..824)
                     /locus_tag="L3M60_001525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226203.1"
                     /translation="MLKIIILSGILSSIIKLPIIGILPTGNIKKNTGINDSKKDGKQR
                     RSNLGISIYQLPLFLYSPINNSVPSNKPIPPEEIRLIKIYLLKSCI"
     gene            complement(999..1748)
                     /gene="nagB"
                     /locus_tag="L3M60_001526"
     CDS             complement(999..1748)
                     /gene="nagB"
                     /locus_tag="L3M60_001526"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBN6226204.1"
                     /translation="MRVLVCKDYDAMSKKAAEMIAAQIVLKPNSILGLATGSTPVGMY
                     RDLVKKYNDNIVDFSDVMSFNLDEYYKLPISNDQSYDYFMKENLFNHVNIKPENTHLP
                     NGMADDIEKECMNYEASIDAAGGIDVQVLGIGRNAHIGFNEPDTKFAKRTHVVELTES
                     TIEANARFFKSREDVPKKAVSMGIGSILKSKKILLLASGEEKADAVYKTVYGEITPEV
                     PGSILQLHKDTIVIVDEAAASKLNPNDYKLV"
     gene            complement(1847..2980)
                     /gene="nagA"
                     /locus_tag="L3M60_001527"
     CDS             complement(1847..2980)
                     /gene="nagA"
                     /locus_tag="L3M60_001527"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBN6226205.1"
                     /translation="MKCLINGKIILKNQILENKVLVFDEKIIDIADSVPKDCEVIDAD
                     GKYISPGLIDIHIHGNMGKDTMDSTDESIETISKSIMRHGVTSFLPTTMTMDKEHVYD
                     ALEVIKKAQNRKLEGAQVLGAHLEGPFINENYKGAQNEKFIINSKYEFIKEYKDVIKV
                     ITYAPEKDIDFDFTREIKRCTDIVLSIGHSNANYEQAKEAINLGVTNVTHMFNAMTGL
                     NHRDPGVVGAALTTNVYSELIADTIHINKDLFQFILNNKGKERLILITDSIEAGGLED
                     GDYSLGGQKVIVKGNEARLENGALAGSVLSLNKMVFNFLDNTNLKVNEAISLASLNPA
                     TSLGINDKKGSLEIGKDADIAVFDENLDCKMTLCLGEVVYKNI"
     gene            complement(2990..3724)
                     /locus_tag="L3M60_001528"
     CDS             complement(2990..3724)
                     /locus_tag="L3M60_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226206.1"
                     /translation="MKIVNKSSNIPLHTQLSSIIREMIETGELKEGDAIMPERELCNI
                     QNVSRMTVNKTIVGLVTEGLLYRVQGKGTFVAKQKKKYQFSNVKGFTDVMKEKGVNIK
                     TDILSFEMELPDDLVKRKLGISDNTTNIYKIVRLRYTDGEPFGLEIVYLSEEMCKGLT
                     KGILDNSSLYRVLNEKYGYKIQKAEQVMEPVILSDEESKLLETDEGALALKLHRNSYN
                     REGNPIEYTISIFRTDKYQYEIVLSE"
     gene            4020..4862
                     /locus_tag="L3M60_001529"
     CDS             4020..4862
                     /locus_tag="L3M60_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBN6226207.1"
                     /translation="MINIGDFNKLTVKRKTEFGYFLDGQTNNTKDDILLHNRLIGKNE
                     INIGDEVNAFIFKDSDDRTAATLIPPLAKVGDVARLKVVDNTDIGTFIDMGLPKDILV
                     PFKAKTYPLFRDEKYLFYIYLDKSKRIAATTDIDSYLLTDHTYNVGDIVTGVVYGFQT
                     NNSAMICVDNKYAGVILHNEYFTELKTGDVLENLHVIKIYEDGKLGLSPRGNRKDELD
                     TLENKILSYLEGSDGYMRFNDKSDPKDISILFNSSKKNFKRALGVLMKKGLIYQDEEG
                     TYLK"
     gene            complement(5043..7373)
                     /locus_tag="L3M60_001530"
     CDS             complement(5043..7373)
                     /locus_tag="L3M60_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6226208.1"
                     /translation="MRSYLELVPQYAKVHKKKNRTTILCIIIAVCLVTAIFGLADMAI
                     QCQKIQFIKDDGNWHIVIRNIDEKFISEINSRVDIAHHGRGQVVDSGEFKSKEILIQG
                     LDEEVAKQFNLEIIRGRYPIGENETLIDEDAVKSFNLKIGDFIKIKLADGRNKNYKIV
                     GEYSSISSLRATDKHGLILSILGTQAIKKSETYDYYYITFKEKVNIRKATSDIKNTFN
                     LSDKQIGKNERLLSIIGQGDSDFSKPLYLTAGLLFVLVLVASIIMIYNSFNMSVIERV
                     KFFGLLRCLGSSKHQVKKFVVLESLILSIKAIPIGLVLGSLVTIISSIFLKYVNELFS
                     SMPILKVSFIGIAFGIIVGFLTVILSAIIPAKKASRVSPLSAVRGNLMSKEILNNGKT
                     SKVANTGSFKIETSMGLNHAFSRKKSFVLMTSSFAISIILFLGFSVLIDLMYQGLKPM
                     KQEAPHISIVDPNFKSVLTKEDINEISNIKGVKKVYSRMMTDIEATYNGKKTKSQLIS
                     YEKYQFNWAKKYLLKGEVNENKLQSENLALVEEGHGFDIGDKIYIKNGNAEKELEVGG
                     ILSSIPFEVKDKYIGNIITSENTFTNITGIKGYIIMDIQVDKDTSGDLIENIRRVVDD
                     NLEVRDKRQSNLEVKNSFYTMATFVYGFVFIIAIISMFNIINSMNISVTSRKNYYGVM
                     RAVGISNRQLRRMVIVEAGSYAVSGCLLGSILGLMLHRYMFISLVSSMFHLEWQVPFN
                     LLLITVLTMIIITLLSVRKPVKKICELDIIDSINAQ"
     gene            complement(7370..8053)
                     /locus_tag="L3M60_001531"
     CDS             complement(7370..8053)
                     /locus_tag="L3M60_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226209.1"
                     /translation="MDILVVNNLCKTYGNGYTKVEALKDVSFTVSKGEFVTVVGESGS
                     GKSTLLNLLGGLDTPTSGRIFIDGKEICSMKEEKQTIFRRRNIGFIFQAYNLIPELTV
                     EQNIIFPLLLDYKKPDTIYLDEVLTILGLKDRKNHLPNQLSGGQQQRVAIGRALITKP
                     SIILADEPTGNLDSKNSNDVISLLKVTAEKYNQTIIMITHNTNIASTADRILQVSDGV
                     LTDLGVNRE"
     gene            complement(8125..9366)
                     /locus_tag="L3M60_001532"
     CDS             complement(8125..9366)
                     /locus_tag="L3M60_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6226210.1"
                     /translation="MKIFSNKDIKMFFLILISILVMFIIVGQIVLISITNNYKKTLIE
                     HDYNIAGYLYSIGVDKSKIPSAFVVHEDKYILKGKEILDTAGYGFDIDVSFTPSVKLF
                     QSKYIKTMSIYLLVFFTMIIFIPYMYFFRQQSKIEEASYKIDSFMNGNINERLNTYEE
                     GSLSKLFSSIDVMSTSLNTHILKEKQSREFLKNTISDISHQLKTPLTALIMYNQILQE
                     ESHNSDVVEAFVQKSQSELERIETLIKNLLKITKIDSKTIVLNKNNVNLKNLIVKILY
                     SFETRAEKEGKVISIKGSENTLLFCDYEWMSEALSNLIKNALDNTKENDKITIEWFET
                     TITTIVSVKDTGNGIHVEDIHHIFKRFYRSKFSKDNQGLGLGLPLVKSIVEQHNGTIS
                     VESKLSQGSTFTISFLKLTNM"
     gene            complement(9377..10060)
                     /locus_tag="L3M60_001533"
     CDS             complement(9377..10060)
                     /locus_tag="L3M60_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226211.1"
                     /translation="MSSILLVEDDLSLIYGLEFSIQKSGFSIDIARTVKEAMQMYEEK
                     NYDLLLLDVSLPDGNGFQICEKVREVSNIPIIFLTASDEEVNVVMGLDMGGDDYITKP
                     FKLNELISRIKALLRRYNVASNVNELSSNNITIKLLENRVFKGEFEVELTTAEYKLLC
                     LFMKNKNIVLTRKNILDKLWDGNGSFIDDNTLSVYVRRLRNKIEDNPENPKFLLTVRR
                     MGYKWNVIK"
     gene            complement(10581..10844)
                     /locus_tag="L3M60_001534"
     CDS             complement(10581..10844)
                     /locus_tag="L3M60_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN6226212.1"
                     /translation="MEETKFCQCCAMPMGQTDELYGTNKDGSKSTDYCSYCFKDGEFT
                     ADISMEEMIEVCIPHMLQSNKDMTEDEARTMMNNFFPTLKRWK"
     gene            complement(10994..11380)
                     /locus_tag="L3M60_001535"
     CDS             complement(10994..11380)
                     /locus_tag="L3M60_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009248914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6226213.1"
                     /translation="MEYKVNDQELNASMFVPFVNRIWPGDYDMDKTQTALSKTLNITA
                     YDDKVLVGCLRVLSDGYYFGTITELLVLPEYQKQGIGSKLFQLAKENTPTMLYFGAQP
                     GVEAFYEKNGCQRSLQSYIIKKKDSY"
     gene            complement(11675..12856)
                     /locus_tag="L3M60_001536"
     CDS             complement(11675..12856)
                     /locus_tag="L3M60_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HBN6226214.1"
                     /translation="MSKDYAKLALEMHEINKGKISVESKVEVKTKDDLSTAYTPGVAE
                     PCLKIHENQDDVYRYTSKGNLVAVVSDGSAVLGLGNIGAEASIPVMEGKAILFKQFAD
                     VDAFPICLKTNDVDEIVKSVELMEPVFGGINLEDISSPRCFEIEERLKKSLSIPVFHD
                     DQHGTAIIVAAAVINSIKLIENKKIEDLEIVINGAGAAGIAIAKILLNMNVKNIILCD
                     RSGALESSVENLNYVQKEMLKVTNIRNEKGPLKDIIKGKDVFIGVSGPGAVTKEMVES
                     MSEKPIILAMANPTPEIMPEEAKLGGAFIMGTGRSDFPNQVNNVLAFPGIFRGALDVK
                     AKEINEEMKIAAAYAIANTISDEEICPEYILPDVFNKNVVKNVAKAVKEAAIKTKVNR
                     I"
     gene            complement(12922..13470)
                     /locus_tag="L3M60_001537"
     CDS             complement(12922..13470)
                     /locus_tag="L3M60_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HBN6226215.1"
                     /translation="MIKITTPVSEIDIAKLNCGDTISLSGILYTARDAAHKRLIDCIN
                     KGEELPFDVYGQGIYYVGPTPTKPGEVIGAAGPTTSYRMDDLTIPLLERGLRLMIGKG
                     KRSDEVIEGMQKYGAVYLAAIGGAGAYISNSIKSCEIIAYEDLGAEAIRKIVVEDLKL
                     TVAIDSYGNNIYEQGRAIYECK"
     gene            complement(13483..14322)
                     /locus_tag="L3M60_001538"
     CDS             complement(13483..14322)
                     /locus_tag="L3M60_001538"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HBN6226216.1"
                     /translation="MRKIKSEQIVEQVKKLCIEASLYLGEDVLGCIKEKAKSEKSEVG
                     KNILNILVENAEIAKEKNIPICQDTGMAVFFVEVGQEVLIEGDTLTDAINEGVRQGYE
                     EGYLRKSVVSPINRVNTKDNTPAVIHYDMVKGDKIKIEFAAKGFGSENMSKMKMLKPS
                     DGLEGIKKFIIDTVSEAGPNPCPPMVIGVGIGGTVDKCAQIAKKALFRELGEFNKDEN
                     IAKLESELLTSINKLGIGPQGLGGTTTALGLNIETFPTHIAGLPVVVNINCHASRHKK
                     VVI"
     gene            complement(14519..14992)
                     /gene="ybaK"
                     /locus_tag="L3M60_001539"
     CDS             complement(14519..14992)
                     /gene="ybaK"
                     /locus_tag="L3M60_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HBN6226217.1"
                     /translation="MVKTNAMRILDSNKIDYKVMSYEVKSEHVDGVEVAHDIGRDVNE
                     VYKTLVTQGVSKNIYVYVIPVHENLDLKKAAKVAKEKSVEMIHVKDINKFTGYIRGGC
                     SPIGMKKLYKTFVNESAKNLDTIIVSAGKIGYQIELSPFDLQRLIKVEFVDVIKK"
     gene            complement(15115..15813)
                     /locus_tag="L3M60_001540"
     CDS             complement(15115..15813)
                     /locus_tag="L3M60_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226218.1"
                     /translation="MEKIRLSIENINKRYDNRIIFRDFNIDFYVNEVNCILGKSGCGK
                     TTLLNIISGIIKNDTNNLNIKENLNKVGNKLEASYIFQDDRLIDWLTVEENIKIVVNK
                     YYNKTKLNKICDEYLELVGISEYKKFYPQMLSGGLRQRVNIARAFIYPSKNIIMDEPF
                     KSIDAKNTQLIMDNFKNILRKEKRTVLFVTHNIEEALFLADRIFILGDSPIRIKKILK
                     NSNELEKNEVLKLI"
     gene            complement(15801..16586)
                     /locus_tag="L3M60_001541"
     CDS             complement(15801..16586)
                     /locus_tag="L3M60_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBN6226219.1"
                     /translation="MKQYTLLRNKKNKEQFIDKMEVTLSCVILLFLWQIIALKIDNDI
                     FLPTVGQVFNSIKEIILNSSFYIDILYSIGRCFFSFLLAMFFAIILSIVSYLNRFFRN
                     LLKPINALARSIPTMILVVLALIWFEKDSTPFIVGFAIVFPILYDNVLGAILNIDKSL
                     LEMASVYKIRFIDKVLKIYLPVIKFQIISILVSTFSLSLKVAIAGEVYSQPTYGIGTM
                     IQTEKINFNTSGIFAWIIIVLLISAILQIAQKFLARRAFLWKR"
     gene            complement(16558..17553)
                     /locus_tag="L3M60_001542"
     CDS             complement(16558..17553)
                     /locus_tag="L3M60_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226220.1"
                     /translation="MKKYISIILLVVMAMVVLVGCSPGKDNSQNKELSVVKQVKVAVP
                     DGLPAVAIAKLANENPEIKDGYETVYSIEKTPEAISTRVMKKDADIAIVPSNMAAIAY
                     NKTSSYNIVGTVGMGSLYLVSTENIKDYSDLVGKEVGCTGKGLTPDITIKSLLLQKDI
                     DYSDINFNYVNSASELVPLLATGKVKTGIVPEPALSALMSKNPDIKIIKSLNDSWKEV
                     SGSKDGYPQSTLIVKSEFLRDNKDFVDSFVGQLSNSIDWANKNPEELGAYSEKIKIST
                     ESKIIGKSLERANLKYIPVKDMIKDYKNYYEKLANFDDKTLGGKVPDEAIYFVEK"
     gene            complement(17659..19593)
                     /locus_tag="L3M60_001543"
     CDS             complement(17659..19593)
                     /locus_tag="L3M60_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBN6226221.1"
                     /translation="MENKFLPISKQDMIDRGWEELDFVLVTGDAYVDHHSFGTAIISR
                     VLENAGYKVGIIAQPDWRTTDDFMKLGKPRLAFLVNAGNMDSMVNHYSVSKKHRDKDM
                     YSPGGKMGYRPDRATIVYCNKIREAYSDVAIVIGGIEASLRRFAHYDYWSDKVRKSML
                     IDSGADLLVYGMSEKQIVEVANALNDGYDPKYIRHIDGTCYISDTLEEIYDKYILIPS
                     YKEICEDKMKYVEAFKIQYDEQDPFRGNIIVQPHGSKYLVQNKPEKPLNREELDEVYG
                     LPYQKTYHPVYEKFGGIPAIEEVKFSIVSSRGCFGSCSFCAITFHQGRAVQSRSEKSI
                     IDEAIGITNLDDFKGYIHDVGGPTANFRRPACNKQITKGACKNRQCLSPSPCKNLDAD
                     HSEYLHLLRAVRKLPKIKKVFVRSGIRYDYVMADKNNKFLRELIEHHVSGQLKVAPEH
                     ISEEVLKYMQKPAGKTYDKFRQKFFAINEELGKKQYLIPYLMSSHPGSTLNSAIELAE
                     YLRDTHYQPEQVQDFYPTPGTLSTTMFYTGIDPLTMKPVYVPKSKRDKAMQRALLQYR
                     APRNYDLVYSALVEAGREDLIGFGHRCLIKPKSEKPYFNRNNSKKNVSKGTNKNKKTN
                     TNNRINKNQQKSSTRKKKKH"
     gene            complement(19778..23326)
                     /locus_tag="L3M60_001544"
     CDS             complement(19778..23326)
                     /locus_tag="L3M60_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6226222.1"
                     /translation="MMNRLKPRILGISILFIIIIILTCGAIFKFKFKLDNKLKETTHT
                     YLSEFTEHNIINLKTRLSGQFDMLESIANFIGNLNNINDKMVIDLMDSNVKRSSLMRM
                     TISTLDGECYSNDGIIRNVQDRDYFKKAKNGERNISEPLKSIVNNKEIIVLSTPVYKS
                     GKIVGVLSGIYDSKKLNELLGLSAFDEKADVFVANKNGAIIAQKTNKNSKTLSCIFQL
                     FNKNNCDEVYCLKTIKENMKNDKSGYFNYDLDNEKFMLNYQPLGVNDWYVFSVVPNKI
                     VSIQSENISYDVYVFALEILLVILIFIIYIACIINNNTKIIINEKEKFKVLTDNILGG
                     VKTCLKDEYMTMTYVSQGFLELTGYSDEDLNKIFKNRFKDMIYKDDLDKFNCIILNQL
                     EKQDKIEVEYRIVKKDGSIIWILERSKSINDNKGSECIQGVLTDITELKKIQEELDSK
                     RFEIQAINDSIVGGIVITEINDDFDSIYLNEGYLNMIGYTRKQLKDELNNKLIKLIFK
                     DDRIGLINSINKQLKLSDTFASEQRVEKREGTIIWILLKGRKIIYENGKSVLCCVIVD
                     ITSAKLLEQELKKSEERHEIILKQTQNIIIEWDIEKDKMNISPNWYNKFKYDPITENA
                     TKNIIKSVDIYHDDMDKFISLIKLTSEGSEYEEREIRLRTRDTTYAWFSIKATTLFDN
                     NGKPYKVVGSLTDIDESKREAEKLKERAEKDPLTDLYNKKASQSLIEEYITLDKKRKG
                     ALMIIDIDDFKGINDNLGHLYGDAVLSEIASDLVNLFRATDIVGRIGGDEFIVFIKDI
                     SETRDIINKAEELVKTFERSFLGKGRKYKISLSVGIAKFPQDGMDYMTLFRNADIALY
                     SAKGKGKNCYTLYDKKIDKVKYIINTRSTEDIDNVEKSFKEHITNYIFDILYESSDIE
                     VAVNLILSIVGKHFNVSRMYIFENSDDNLVCKNTFEWCNEGITSEINNLKNIYYKDLG
                     NYLLNFNADGLFYCNDIENLGSDVKKVLKPQGIRSILQCKILEGSEFRGFIGFDECSY
                     NRMWTNDEVNTLTFISKTLGMFLLKMRIQSKLEESLTIQKSVMDNMDIWAYVVDRNTY
                     ELLFINKKMLESAPNTHVGDLCYKAIWNNQNKPCKKCPMIYLKDGDDTYKSFMRNKKF
                     EIQANITTTTLKWFDGKDACLIFGSEVTSIESEENT"
BASE COUNT         7661 a         4147 c         2508 g         9480 t
ORIGIN      
        1 attataccag ctttttatag tacattagtt ttaaaattgt ttggatttta aggcaatttt
       61 aaggcaaaaa ataaattatt atagaattta ttaaactcat tttagaaata tcttgaacta
      121 cttcacttta attacctttt attactttag gtgacatttg aaagtgtagt actttgcata
      181 tttttgataa aataataatt caatattcaa ctgtatttat tcattttata tcaaaattga
      241 tatttttcaa actttttatt gaatattcaa acaattaata ttatagttat attaagtact
      301 acagttcacc ttaattctaa aaatatttat tcatatatag tacttttttc attcttcact
      361 aatattatgg atttcttaaa ctaaatgata aaatcatatt ccaacaaaat aaaactggat
      421 aaagtatttc tgaagggaga atttaaaatg aaaaaaactc ttagatattt tttattttta
      481 attattctat tgcttatgta tttcttagct gttaaattaa attatgattt ttatttccga
      541 tttcacactg gttatatgca agactttaaa agatatattt ttattaacct aatttcctct
      601 ggtggtattg gtttattact aggaactgaa ttattgattg gtgaatacaa aaaaagtggt
      661 agttggtata ttgatatccc aagattactt cttctttgtt ttccatcttt tttactatca
      721 ttgatgcctg tattcttctt tatgtttcca gttggtaata ttccgattat aggtaatttt
      781 attatacttg atagaatccc actgagtata ataattttta acatcttatt tggatacttc
      841 ttgataacga gttttcgtaa aaagtaataa acaagtaatt taaacaataa taactaattt
      901 aaatgaaaaa aagtaagtga tttaaataat aatagctatc attaaataaa tttgattatt
      961 caatgatagc tattttttac ttaaactaca ttctattatt atactaattt ataatcgttt
     1021 ggattcaatt tacttgctgc tgcttcatct actataacta tagtatcctt atgaagttga
     1081 agtatagaac caggaacttc tggagttatc tctccataaa cagtcttata taccgcatca
     1141 gctttttctt ctcctgaagc taaaagtaat attttcttag attttaatat tgaacctatt
     1201 cccatactta cagccttttt aggaacatct tctcttgatt taaagaatct tgcattagct
     1261 tctattgtag attctgtaag ttcaactaca tgagttcttt ttgcaaattt agtatcaggt
     1321 tcattaaaac ctatatgagc attacgacct attccaagta cttgtacatc tataccacca
     1381 gctgcatcta tagaagcttc atagttcata cactcttttt caatatcatc agccattcca
     1441 tttggtaaat gagtattttc tggctttatg tttacatgat taaatagatt ctctttcata
     1501 aaataatcat aactttggtc attagatatt ggtaacttat aatattcatc caaattaaag
     1561 ctcatcacat ctgaaaaatc tactatatta tcattgtatt tttttaccaa atctctatac
     1621 atacctactg gtgtacttcc tgtagctagt cctaatattg aatttggttt taaaactatt
     1681 tgagctgcta tcatctcagc tgctttttta ctcattgcat cataatcttt acacactaat
     1741 actctcatct tgttcctcct aaattttaac tactatttca actaaggtaa gtttaattta
     1801 ttatttttta tattgctttt actgtcttta aagtttgttc tgtatactat atatttttat
     1861 atactacttc ccccaagcaa agtgtcattt tacaatctaa attctcatca aatacagcta
     1921 tatctgcatc cttgcctatt tctaagcttc cttttttgtc atttattccc aaagatgttg
     1981 ctggatttaa agatgctaaa ctaatcgctt cattcacctt aaggtttgta ttatctaaga
     2041 agttaaatac cattttattt aaagacaaca cacttccagc taatgctcca ttttcaagtc
     2101 tagcttcatt ccccttaact atgactttct gacctccaag agaataatct ccatcttcaa
     2161 gaccaccagc ttctatagaa tctgttatta aaatcaatct ttctttacct ttattgttta
     2221 gtataaattg aaataaatct ttatttatat ggattgtatc tgctattagt tcactgtaca
     2281 cattagttgt aagtgctgca ccaacaacgc ctgggtctct gtggtttaaa ccagtcattg
     2341 cattgaacat atgagttaca tttgttacac ctaaatttat agcttcttta gcttgctcat
     2401 aatttgcatt actatgacca atagataaaa ctatatctgt acatcttttt atttctctag
     2461 taaaatcaaa atcaatatct ttttctggag cataagttat aacttttata acatctttat
     2521 attcttttat aaattcgtat tttgagttta ttataaactt ttcattttgt gctcccttat
     2581 aattctcatt tataaaaggt ccttctaaat gtgctcctaa tacttgtgcc ccttcaagct
     2641 ttctattttg ggctttttta ataacttcaa gtgcatcata aacatgttct ttatccatag
     2701 tcattgtagt tggaagaaaa gatgttactc catgtctcat tattgacttt gaaattgttt
     2761 caatagattc atcagttgaa tccattgtat cttttcccat gtttccatgt atatgaatat
     2821 caataagccc tggagatata tattttccat cagcatctat aacttcacaa tcttttggta
     2881 cactatcagc tatatcaatt attttttcat caaatactag tactttattt tccaagattt
     2941 gattttttaa tattatcttt ccattaatta aacatttcat attattcacc tactcactca
     3001 atactatttc atattgatat ttatctgttc taaatattga tatggtatat tctatagggt
     3061 taccttctct attataggaa tttctatgaa gtttaagagc taaagcacct tcatctgttt
     3121 caagaagctt actttcttca tctgaaagta ttactggctc cattacctgt tcagcttttt
     3181 gtatcttata gccatatttt tcatttaaaa ccctatataa tgaagaatta tccaatatcc
     3241 cttttgttag acccttacac atttcttctg atagatatac tatttctaat ccaaatggct
     3301 ctccgtcagt atatctaagt ctgacaatct tatatatatt tgtagtatta tcacttattc
     3361 caagctttct tttaactaaa tcatctggta gttccatctc aaaggataat atatctgttt
     3421 ttatgtttac tcccttttct ttcataacat cagtaaaacc ttttacattt gaaaactgat
     3481 acttcttttt ttgctttgca acgaatgtcc ctttaccttg taccctataa agaagtcctt
     3541 ctgttactaa tccaacaata gttttattta ctgtcatacg acttacattt tgtatgttac
     3601 aaagttctct ttctggcatt attgcatcac cttctttaag ctcaccagtt tctatcatct
     3661 ctcttattat tgaacttaat tgagtatgca atggtatgtt actagattta tttactattt
     3721 tcatattaat caccactttt caatatggta tataccaatt ttggtatata ccaattatac
     3781 tttaaaagaa attttatttc aatacttttt aatttatttt gtaaaaattt tagtaaaacc
     3841 taaagacaaa aaagaaattg gagtttatac tatataagaa gctagagaat tgaaaaatac
     3901 ataaataact atattattta ttgtaaattt cactaatact aatatagata ctgtgacaaa
     3961 ctactacata agtatattat ggctaaatta taaacataaa atgagaggat gattttaatt
     4021 tgataaacat aggtgatttt aataaactta cagtaaaaag aaaaactgaa tttggatatt
     4081 ttttggatgg acaaactaat aacacaaaag atgatatact tcttcataat agattaatcg
     4141 gaaaaaatga aataaacata ggtgatgaag taaatgcttt catatttaaa gattctgatg
     4201 acagaactgc tgcaacttta ataccgcctt tagctaaggt tggggatgta gcacgcttaa
     4261 aagtagttga taatacagac attggtactt ttatagatat gggacttcct aaagatatac
     4321 tagttccttt taaagctaag acttatccac tatttagaga cgaaaaatat ttattttata
     4381 tatatttaga caaaagcaaa agaattgctg ctactacaga tatagattca tacttattaa
     4441 cagaccatac ttacaatgta ggagatattg taactggagt tgtatatgga ttccaaacta
     4501 ataattctgc aatgatatgt gtagacaaca aatatgctgg tgtaatactt cataatgagt
     4561 actttactga actaaaaact ggtgatgttc ttgaaaattt acatgtcata aaaatatatg
     4621 aagatggaaa acttggtctt tctcctagag gaaatagaaa ggatgaactt gatactttag
     4681 aaaataaaat tttaagttat ttagagggtt ctgatggata tatgagattt aatgataagt
     4741 ctgaccctaa agatatttct atattattta attcaagtaa gaaaaatttt aaacgagctc
     4801 ttggagtgct tatgaaaaaa ggattaattt atcaagatga agaaggaact tatctaaaat
     4861 agtatattta aaatatttta acttaaatgc tcacgttgtc atataatgtt tagattcaca
     4921 tcatctttat agaaattttg taacataaca aaaattttac aatataaaaa acgaagtaaa
     4981 aatgaaaaat attttctatt tcatttttac tttgtttttt taatttaaat caatagctat
     5041 atttattgtg catttattga gtctattata tctaattcac aaatcttttt gactggtttt
     5101 cttactgata ataaagttat tattatcata gttagtacag tgataagtag taaattaaaa
     5161 ggtacttgcc actctaaatg aaacattgag cttactaacg atataaacat atacctatgt
     5221 aacattaatc ctagtatact acctaataaa caaccactca ctgcatatga acctgcctct
     5281 actattacca ttcttcttaa ttgtctgttg gatatgccta ctgcacgcat aacaccatag
     5341 taattctttc tacttgtaac acttatattc atgctattta tgatattaaa catgcttata
     5401 atcgcaataa taaacacaaa tccatataca aatgttgcca tagtataaaa cgaattttta
     5461 acctctaagt tactttgtct tttatctctg acctctaagt tatcatctac tacacgtctt
     5521 atattttcaa ttaaatctcc agaagtatct ttatcaacct gaatgtccat tattatataa
     5581 ccctttatac ctgttatatt cgtaaaagta ttttcagatg ttataatatt tccaatatat
     5641 ttatctttaa cctcaaatgg aattgaggaa agtatacctc ctacctctaa ctctttttca
     5701 gcatttccat ttttaatata tatcttatct cctatatcaa atccatgccc ctcttctact
     5761 aatgcaaggt tttctgactg tagtttattt tcattaactt ctccttttaa tagatatttt
     5821 tttgcccaat taaattgata cttttcataa gaaattaatt gtgattttgt cttcttccca
     5881 ttatatgtag cttctatatc cgtcatcatt ctactgtata ctttctttac tcctttgata
     5941 ttacttattt cattaatatc ttccttagtt aaaacagact taaaattagg gtcaacaata
     6001 gatatatgag gagcttcttg cttcataggc ttaagtcctt gatacataag gtctataagc
     6061 acactaaaac ctaaaaatag tataatacta atggcaaatg aagaagtcat aagtacaaaa
     6121 gatttctttc ttgaaaaagc atgatttaat cccatagaag tctcaatttt aaaacttcct
     6181 gtatttgcaa ctttacttgt ctttccgtta tttaaaattt ctttggacat tagattccca
     6241 cgtacagcgc taagtggaga tacacgggaa gctttttttg ctggaataat agctgataat
     6301 attacagtta aaaaccctac aattattcca aatgcaatac caataaaact aacttttaaa
     6361 attggcattg agctgaataa ctcattaaca tacttaagaa atatagaaga tataatagtt
     6421 acaagacttc ctaatactaa gcctattgga atagctttta tacttaaaat taaactttct
     6481 aaaacaacaa attttttaac ttgatgtttt gatgacccta gacaacgtaa caaaccaaaa
     6541 aatttaactc tctcaattac actcatgtta aaactattat atatcatgat aatacttgct
     6601 actaaaacaa gtacaaaaag taatcctgct gttaaataca gtggtttaga aaagtcagag
     6661 tcaccttgac ctataataga aagtaatctt tcatttttac cgatttgttt atcacttaaa
     6721 ttgaaggtat ttttaatatc agaagtggct tttctaatat taaccttttc tttaaatgta
     6781 atataataat aatcgtacgt ttctgatttt ttaatagctt gtgtacccaa tattgaaagt
     6841 attaatccat gtttatctgt agctcttaag cttgatattg atgaatattc acctactatc
     6901 ttataatttt tatttctacc atctgctaat tttattttga taaaatctcc tatttttaga
     6961 ttaaagcttt taactgcatc ctcatctatt aaagtctcat tctccccaat aggataacga
     7021 ccccttatta tttctaagtt aaattgtttt gctacttctt catctaaacc ttgtatcaaa
     7081 atctcctttg atttaaactc tccactgtca acgacttgcc ccctaccatg gtgagctata
     7141 tctactctac tatttatttc tgatataaac ttttcatcaa tatttctaat tactatgtgc
     7201 caatttccat catctttaat aaattgtatc ttttgacatt gtatagccat atctgcaagt
     7261 ccaaagatag cagtaactaa gcatactgca ataataatac ataaaattgt agttctattt
     7321 tttttcttat gtacttttgc atattgtggt actaattcta agtagcttct cattctctat
     7381 tcacccccag gtctgtaagc acaccatcag aaacctgtag tattctatct gctgtagaag
     7441 ctatattcgt attatgagtt atcataataa tggtttgatt atacttttct gcagtcactt
     7501 ttaaaagtga aattacatca ttactatttt tactgtcaag atttcccgtt ggctcatctg
     7561 ccagtataat acttggcttt gtaatcaaag cccttccaat tgcaactctc tgttgttgac
     7621 caccagataa ttgatttgga agatgatttt tcctatcttt aagacctaaa atagtcaata
     7681 cttcatctaa gtaaatagta tctggttttt tataatctaa taacaatggg aatattatat
     7741 tttgctccac agttagttct ggtataaggt tatatgcctg aaatataaac cctatgttac
     7801 gacgtctaaa aatagtctgt ttttcctctt tcattgaaca tatttcttta ccatcaataa
     7861 atattctccc tgatgttgga gtatcaagac ctcctaagag atttaatagt gtactctttc
     7921 ctgaaccaga ttctccaact acagtcacaa actctccttt tgaaactgta aaggatacat
     7981 ctttcaatgc ctctactttt gtatatccat ttccatatgt tttacataaa ttattaacca
     8041 caagaatatc cattttctct ctcacctttc taattgatta atataataat aatctatata
     8101 ccttactgca agatgtattt tatcttacat attagtaagc tttaaaaaac ttatggtgaa
     8161 tgtacttcct tgtgatagtt tactttctac agaaatagta ccattatgtt gttccacaat
     8221 agatttaact agtggtaatc ccagtcctaa accttgatta tcttttgaaa acttacttct
     8281 ataaaacctt ttaaatatat gatgaatatc ctcaacatgg attccatttc cagtgtcttt
     8341 aactgaaaca atggtggtta tagtcgtctc aaaccactct atcgttattt tatcattctc
     8401 ttttgtatta tctaatgcat tctttataag attacttaaa gcctctgaca tccattcata
     8461 atcgcagaac aacaatgtat tttctgaacc ctttatagaa ataacctttc cttctttttc
     8521 tgccctagtt tcaaagctat acaaaatttt gacaattaaa tttttaagat taacattatt
     8581 tttattgaga actattgttt ttgaatctat ctttgtaatc tttagtaaat tcttaatcaa
     8641 tgtttcaatg cgttctagtt cactttgact tttttgtaca aaagcttcaa caacatcact
     8701 attatgactt tcttcttgta gtatctgatt gtacattatt aaagctgtaa gtggagtttt
     8761 taattgatgc gatatatccg aaattgtatt tttaagaaat tctctgctct gtttttcttt
     8821 taatatatgt gtatttaaag atgtcgacat aacatctatt gaactaaata attttgataa
     8881 gctaccttct tcatatgtat ttagtctttc atttatattt ccattcataa atgaatcaat
     8941 tttataacta gcctcttcta tcttactttg ttgcctaaaa aaatacatat atggtatgaa
     9001 aataatcata gtaaaaaata ctaataaata gatagacata gtttttatat atttactttg
     9061 aaacaatttt acacttggtg taaagctaac atctatatca aatccatatc cagctgtatc
     9121 tagtatttct tttcctttta aaatatattt atcttcatgt acaacaaatg ctgatggtat
     9181 tttagattta tcaacaccta tactatataa gtatccagct atattataat catgttctat
     9241 caacgttttt ttataattgt tagttatact tataagcact atttgcccta ctataataaa
     9301 cattactaaa atagatataa gaattaaaaa aaacattttt atatctttat tagaaaatat
     9361 cttcatatta ataccctcac ttaattacat tccatttata acccattctt ctaacggtca
     9421 atagaaactt agggttttca ggattatctt caattttatt tctcaatcgt cttacatata
     9481 ctgataatgt attgtcatct ataaagctac catttccatc ccataacttg tccaatatgt
     9541 tttttctagt aagtacaata tttttatttt tcataaataa gcacaataat ttatattctg
     9601 ctgtggtaag ttccacctca aattcacctt taaaaacacg attttcaagt aatttaatag
     9661 taatattatt tgaacttaat tcattcacat tacttgcaac attataacgt ctaagtaatg
     9721 cttttattct tgaaattaat tcatttaatt taaaaggttt tgttatataa tcgtcgccac
     9781 ccatatcaag ccccatcact acatttacct cttcatctga agcagtcaaa aatataatgg
     9841 gtatattaga aacctctcgt actttttcgc aaatctgaaa tccattacca tcaggtaggg
     9901 atacatctaa aagcaataag tcataatttt tttcctcata catttgcata gcttctttta
     9961 ctgtccttgc tatgtctata ctaaatccac ttttttgtat agagaactct aatccatata
    10021 tcaagcttaa atcatcttca actaacaata tactactcat ttaatcacct ctattttaat
    10081 tatttttact tctaatacat tattacactt aatttatttt aattataatt ttactttttc
    10141 aaaaactcaa tatattaaaa caaatgtttt tacaaaagac tacacaaatc aacagtctaa
    10201 tgattggtat tactcctata actctcatcc ctctttctat ctcaaatagc tagaaatcag
    10261 cttgacctgt attattttat ataaaaaata gaaaatttta taattcttta tctgttaata
    10321 gttaaacaga gaattgaaaa taatgatttt gtagttggat ttgacttgag gtataaaaag
    10381 acatggatat agaagtagaa tataaataac atattttaga ttttcacaat ttgaaataga
    10441 aagagggatg agaacatatt taagcataca agaataactg aactagcaag taacaaaaca
    10501 ggtggtatgc tataaagcac accacccctt attctcaaca ccatatttca tgttttgcat
    10561 ataactaatt ttgcataaaa ttatttccag cgttttaatg ttggaaagaa attgttcatc
    10621 attgttcttg cttcatcttc agtcatatcc ttattacttt gaagcatatg agggatacaa
    10681 acttctatca tctcttccat acttatatca gctgtaaact caccatcttt aaaacaataa
    10741 ctacaataat cagtactttt acttccatct ttatttgtcc catataattc atcagtttgt
    10801 cccattggca ttgcacagca ttggcaaaat ttagtttctt ccattttaaa tcactcctta
    10861 tatttttctt tggctcttaa ccatattaaa agtatatcaa tactaatatg acatgtttat
    10921 gtcatattag taataaaaaa tgaaatgatt ccactaaaat attgtacttt taagattatt
    10981 gaaagctgga actttaataa ctatctttct tttttattat gtaggattgc agacttcttt
    11041 ggcacccatt tttttcatag aacgcttcta ctcctggctg tgcaccaaaa tatagcatag
    11101 ttggggtgtt ctctttagca agctggaaaa gcttactacc aatgccttgt ttctgatact
    11161 ctggaagaac aagcaattct gtaattgttc caaaataata accatctgaa agaactcgca
    11221 gacatcctac aagcacttta tcatcataag cagttatatt caaagttttt gataatgcag
    11281 tctgtgtttt atccatatca taatctcctg gccatatcct gttgacgaat gggacaaaca
    11341 ttgaagcatt aagttcttgg tcattaactt tatattccat catctcttgt acctccataa
    11401 atttaaattt attaatttat aatattcata ttatatcatt ctcatatata cgcaacaata
    11461 aaaatctgcc ttcctatatt tttttctata aacctttaca accatgtatc tactaaagta
    11521 ataaagatag agtctgatag tataaaattt actacatttt ctaacacctt taatcataaa
    11581 gatatgtata taaaagtaag gatatatgag tataaaccta taataaaaat gcccatatac
    11641 ccgataagat aaggatatat gaacatataa aaatttatat tctatttact ttggttttta
    11701 tcgctgcttc tttaactgcc ttagcaacat tttttacaac attcttatta aatacgtctg
    11761 gcaatatata ctctgggcat atttcttcat cacttatagt attagctatt gcatatgcag
    11821 cagctatctt catctcttca tttatctctt tagccttgac atctagtgca cctctaaata
    11881 ttccaggaaa agccaagaca ttattaacct gattagggaa gtctgaacga cctgttccca
    11941 ttataaatgc tccacctaat tttgcttctt ctggcattat ctctggagta ggatttgcca
    12001 ttgccaatat tattggcttt tcagacatac tttcaaccat ttcttttgta acagctcctg
    12061 gacctgaaac accaataaat acatcttttc cttttatgat atcttttaaa ggtccttttt
    12121 catttcttat atttgttact tttaacattt ctttttgaac atagtttaga ttttctacac
    12181 tagattccaa tgcaccactt ctatcacata agattatatt cttaacattc atattaagta
    12241 gtatctttgc tattgctata ccagcagccc ctgctccgtt tataactatt tctaaatctt
    12301 ctattttttt gttttcaatt aactttattg aatttattac agctgctgca actattattg
    12361 ctgtcccatg ttggtcatca tgaaaaacag gtatacttaa agattttttt agcctctctt
    12421 ctatctcaaa acatcttgga gaagatatgt cttccaaatt tatacctcca aatacaggtt
    12481 ccatcaactc tacagattta actatctcat ctacatcatt agtctttaaa catattggaa
    12541 aagcgtctac atctgcaaat tgtttaaata atatggcctt tccttccata acaggtatag
    12601 atgcttcagc accaatgtta cctaatccaa gtacagcact tccatcacta actactgcta
    12661 ctagattccc tttagaagtg tatctataaa catcatcttg attttcatgt atttttaaac
    12721 aaggttctgc aacacctggt gtatatgctg ttgataaatc gtccttagtt ttaacttcta
    12781 ccttgctttc tacacttatt tttcctttgt tgatttcatg catttctaat gccaattttg
    12841 cataatcctt agacatcaat tttcctcctt aaaatataca caactatttt taattttaat
    12901 attcatataa atttctaaac tttatttaca ttcataaatt gctcgacctt gttcatatat
    12961 attattacca tagctatcta tggctactgt aagtttaagg tcttctacta caatctttct
    13021 tatagcttcg gctcctaaat cttcatatgc aataatttca catgatttta ttgaatttga
    13081 aatatatgct ccagctccac ctattgcagc aagataaact gctccatatt tttgcatacc
    13141 ttctataact tcgtcacttc tttttccctt acctatcatc aacctaagac ctctctctaa
    13201 aagaggtata gtcaaatcat ccattctata gcttgtcgtt ggtccagcag cacctataac
    13261 ttctcctggc tttgtaggag taggtcctac ataatatatt ccttgaccat atacgtcaaa
    13321 tggtaattct tcacctttat ttatacagtc tatcaatctt ttatgagcag catctcttgc
    13381 tgtataaagt ataccactca agctaattgt atctccacaa tttaattttg ctatatcaat
    13441 ctcacttaca ggtgtagtta tttttatcat tttattttcc tcctaaatta ctaccttctt
    13501 atgtcttgat gcatggcagt ttatatttac aacaacaggt agacctgcta tgtgtgtagg
    13561 aaaagtctct atgtttaatc ccaaagctgt tgtagtacca cctaaacctt gaggccctat
    13621 accaagttta ttaatagatg ttaatagttc actttcaagc tttgctatat tttcatcctt
    13681 attaaattca cctagttctc taaaaagtgc ctttttagct atttgagcac atttgtctac
    13741 agtcccacct ataccaactc caattaccat aggagggcat gggttaggtc cagcttctga
    13801 aactgtatct attatgaatt ttttaattcc ttctagtcca tctgatggtt ttaacatttt
    13861 catcttactc atattttcgc ttccaaaccc cttggctgca aattctattt taattttatc
    13921 accttttacc atatcataat gaataacagc aggtgtatta tcttttgtat ttactctatt
    13981 aattgggctt acaacagatt ttcttaagta gccttcttca tagccttgtc ttactccctc
    14041 atttatcgca tcagtaagcg tatctccttc aattaaaact tcttgtccta cttcaacaaa
    14101 aaatacagcc ataccagtat cttggcatat tggtatattt ttttctttag ctatttcagc
    14161 attttcaact aatatattta atatattttt tccaacttca ctcttttcgc ttttagcttt
    14221 ctcttttata caacctaaaa catcttctcc taaatatagg ctcgcttcta tacaaagttt
    14281 ttttacttgt tcgactattt gttcagattt tatttttctc aagcgaacat ccctccatta
    14341 ctttataaaa aatattcgtg attacactta cactttacca ttgtttttaa aatttctcaa
    14401 tatttttata tcaattacac ataagtaatt gaatatttat tttaaaatta ttattcattt
    14461 tattaacact acaacacctg ttattaagaa tataatctct atattttata tgtgattctt
    14521 atttttttat tacatctaca aattctactt ttataagtct ttgtaaatca aatggagata
    14581 gttctatttg atatcctatt tttcctgcac tcacaataat tgtatctaaa ttctttgcac
    14641 tttcatttac aaaagtttta taaagctttt tcatgcctat aggagaacat ccacctctaa
    14701 tataacctgt aaacttattt atatctttta catgaatcat ctctacactt ttctctttag
    14761 ctacttttgc agcttttttt aaatctaagt tttcatgtac tggtattaca tatacatata
    14821 tattcttact aactccttga gttaccaatg tcttatacac ttcatttaca tctcttccta
    14881 tatcatgtgc cacctcaaca ccatccacat gttcactttt cacttcataa gacattactt
    14941 tataatcaat cttatttgaa tctaatattc tcatagcatt agtcttaacc atatcttcac
    15001 ttccttttta taaaatacga acattataca catttttgaa tattctgtac catattaaca
    15061 gagtcttata tattttaaac aaatatgttt taaaacgata gtattattat aactctatat
    15121 caacttcaaa acttcatttt tttccaattc attagaattt tttaatattt tttttattct
    15181 aattggagaa tctcctaaaa taaatatcct atctgctaaa aacaatgctt cctcaatatt
    15241 atgagttaca aataacacag ttctcttctc ttttcttaat atatttttga aattatccat
    15301 tataagctgt gtattctttg catcaataga tttaaaaggt tcatccatta taatgttttt
    15361 ggatggatat ataaaagctc tagcaatatt taccctttgt ctaagacctc cactaagcat
    15421 ctgaggatag aattttttat actcactaat tccaaccaat tctaagtatt catcacaaat
    15481 tttatttaat ttagttttat tatagtattt atttacaact atctttatat tttcttctac
    15541 tgttaaccag tctataagtc tatcatcttg aaatatgtaa ctagcttcaa gtttatttcc
    15601 tactttattt aaattttctt tgatatttag attgttggta tcatttttaa taatcccact
    15661 tattatattc aagagtgtag tttttccaca accagatttt ccaaggatac aatttacctc
    15721 atttacataa aaatcaatat taaaatctct aaatataata cgattatcat atcttttatt
    15781 tatattctca atacttaacc ttatcttttc cataaaaaag ctctccttgc taaaaacttt
    15841 tgagctattt gcaaaatggc agaaattaaa agaacaataa ttatccatgc aaatattcca
    15901 gatgtattga agttaatttt ttcagtctgt atcatagttc caataccata tgtaggttga
    15961 ctatatactt ctcctgctat agcaactttt aaacttaaag agaatgtcga tactagtata
    16021 gatataattt gaaattttat aactggtaaa taaattttta ataccttatc tataaacctt
    16081 attttataca cactagccat ttctagtaag ctcttgtcta tattcaatat agctcccaaa
    16141 acgttatcat ataatatagg gaaaacgata gcaaatccaa ctataaaagg agtactatcc
    16201 ttttcaaacc aaataagtgc aagtactacc aatatcatag ttggtattga acgtgccaaa
    16261 gcattgattg gttttaataa atttctaaaa aatctattta aataagacac tattgaaagt
    16321 ataatggcaa aaaacatagc cagtaaaaaa ctaaaaaaac atctccctat agagtaaagt
    16381 atatctatat agaaactaga atttagtata atttctttta tgctattaaa tacttgacct
    16441 actgttggta aaaaaatatc attatcaatt tttaaagcaa taatttgcca taaaaataag
    16501 aggataacac aagaaagtgt tacctccatt ttatctataa attgttcttt atttttttta
    16561 tttctcaaca aagtatattg cttcatctgg tactttgcct cctaatgtct tatcatcaaa
    16621 attagctaat ttctcatagt aatttttata gtctttaatc atgtctttaa ctggaatata
    16681 ttttaaatta gctctttcaa gagattttcc tattatcttt gattcagtag atatttttat
    16741 tttttcagaa tatgcaccta attcttcagg atttttattt gcccaatcta tactatttga
    16801 taattgacct acaaaagaat caacaaaatc tttattatct ctcaaaaact cagattttac
    16861 aattaaagtg gattgaggat aaccatcttt tgagccactt acttccttcc atgagtcatt
    16921 taaacttttt ataattttta tatctggatt tttagacatc aaagcactaa gtgcaggttc
    16981 tggaacaata cctgttttaa ctttaccagt agccaataat ggtactaatt cacttgctga
    17041 atttacataa ttaaaattta tatctgaata atctatatcc ttttgtaaca aaaggctttt
    17101 tatagttata tctggagtaa gtccttttcc agtacatcca acttctttac caactaaatc
    17161 agaatagtct tttatatttt ctgttgatac caaataaagt gaacccattc ccacagttcc
    17221 aactatatta tatgatgaag ttttattata agctatcgca gccatgtttg aaggaactat
    17281 agctatatct gcatcttttt tcatcactct agtggaaatc gcctcaggtg tcttttcaat
    17341 gctatacact gtttcatatc catctttaat ctctggattt tcatttgcaa gtttggctat
    17401 tgccactgct ggtagcccat ctggaacagc tactttcact tgtttgacaa ctgataactc
    17461 cttattttga gaattatctt ttcctggtga acatcctacc aaaacaacca tagccatcac
    17521 cactaataat attatagaaa tatacttttt catctcttcc tcccaaaggt aacgccttta
    17581 aaataattat atttatttat actaatttct gttaatttat cgaataatcc ttctttttta
    17641 agtattttta tttacacttt aatgcttttt cttttttcta gttgaacttt tttgttgatt
    17701 tttattaatt ctgttatttg tattagtttt tttgttttta tttgttcctt tagatacatt
    17761 ttttttagaa ttatttctat taaaataagg cttctcactt tttggcttta taaggcatct
    17821 atgtccaaaa ccaattaagt cttctcttcc tgcttcaact aatgcagaat ataccaaatc
    17881 ataatttcta ggtgctctat attgtaataa agccctttgc attgccttgt cacgttttga
    17941 tttgggaaca taaactggct tcattgtaag tgggtcaata cctgtataaa acatggttgt
    18001 agatagagtc cctggagttg gataaaaatc ctgaacctgc tctggttgat agtgagtatc
    18061 tcttaaatac tctgctaact ctatagcact atttaaggta gaaccaggat gactagacat
    18121 taaataaggt atcaaatatt gctttttacc taattcttca tttatagcaa aaaacttctg
    18181 tctaaattta tcataggttt ttcctgctgg tttctgcata tactttaaaa cttcttctga
    18241 tatatgttct ggagctacct ttaactgacc acttacatga tgctctatca attctctaag
    18301 aaatttattg tttttatctg ccattacata atcgtatcta attccagaac gcacaaatac
    18361 cttttttatt ttaggtaatt ttcttacagc tctaagtaaa tgtaggtatt cactgtggtc
    18421 tgcatctaga tttttacatg gtgatggaga taaacattgt ctatttttac aagcaccttt
    18481 agtaatttgt ttgttacatg ctggtcttct aaaattagct gttggacctc caacatcatg
    18541 tatataccct ttaaaatcat ctaaattggt tattcctatt gcttcatcaa taattgattt
    18601 ctcacttcta ctttgtactg ccctaccttg atgaaatgtt attgcacaaa acgaacaact
    18661 accaaagcat cctcttgaac ttactatact aaattttact tcctctatag ctggtatacc
    18721 accaaatttt tcatatacag ggtggtatgt cttttgatat ggcaatccat atacctcatc
    18781 taattcttct ctgttaagtg gtttctctgg tttattttga accagatact tacttccgtg
    18841 aggctgaaca attatatttc ctctaaacgg gtcttgctca tcatattgaa ttttaaaagc
    18901 ttctacatac ttcattttat cttcacatat ttctttataa gatggaatta atatatattt
    18961 atcatatatt tcttccaaag tatctgaaat atagcatgtc ccatcaatat gtctaatata
    19021 tttcgggtca tatccatcat taagtgcatt ggctacttct acaatctgct tttcactcat
    19081 accataaact aataaatctg ctccactatc tattaacata gattttctga ccttatcact
    19141 ccaataatca tagtgtgcaa atcttcttaa actcgcttca attccaccta ttactattgc
    19201 gacatcactg tatgcttctc ttattttatt acaatatact atagtagctc tatctggtct
    19261 atatcccatt ttcccacctg gagaatacat gtccttgtct ctatgctttt tacttacaga
    19321 ataatgatta accattgaat ccatatttcc tgcattaact agaaatgcaa gtctaggttt
    19381 tcctagcttc ataaaatcat ctgtagttct ccagtctggt tgagctatta ttccaacttt
    19441 atacccagca ttttctagaa ctctagatat tattgctgtt ccaaaactat gatggtctac
    19501 ataagcatct cctgtaacta gaacaaaatc taattcttcc catcctctat ctatcatatc
    19561 ttgtttgctt attggtaaaa acttattttc catattatca cctgctcaat atatatttaa
    19621 caaatatttt tttatacaaa agtatctttt aattttgttc ttaacgctta attatatcat
    19681 aaaaaatact taaaaccaca acttaaatga tttatataga cattttaagt ctaataaatt
    19741 caaattgcaa ttttaagtat atatataaca cagtatttta agtattttct tcagattcaa
    19801 tactagttac ttcgcttcca aatattaaac atgcatcttt accatcaaac catttcaaag
    19861 tagttgtagt tatatttgcc tgtatctcaa actttttatt acgcataaat gatttataag
    19921 tatcatcacc atcttttaaa tatatcatag gacatttttt acaaggttta ttttgattat
    19981 tccatatcgc tttataacac aaatcaccta catgagtgtt tggtgctgac tcaagcattt
    20041 ttttgttgat aaataataat tcataggtat tcctgtcaac aacatatgcc catatgtcca
    20101 tattatccat tacagacttt tgaatcgtta aagactcttc tagcttactt tgaatacgca
    20161 tttttaataa aaacatgccg agtgttttag atataaaggt caatgtattc acttcatcat
    20221 tagtccacat acgattatat gaacattcat caaatcctat gaaccctcta aattcactgc
    20281 cctctaaaat tttacactgt aatattgaac gtataccttg tggtttaagt acttttttca
    20341 catcactccc taaattttca atatcattac aataaaatag cccatctgca ttaaagttta
    20401 aaagatagtt acctaaatcc ttgtaataaa tatttttaag attattaatt tcagaagtta
    20461 taccttcgtt acaccattca aatgtatttt tacatactag attatcatct gaattttcaa
    20521 atatatacat acgactcaca ttaaagtgtt tacctacaat acttagaatt aaatttactg
    20581 ccacttctat atcacttgat tcatatagta tatcaaatat ataatttgta atatgttctt
    20641 taaaagactt ctctacattg tcaatatctt ctgtacttct tgtattaatt atatatttaa
    20701 ctttatcaat ttttttatca tacaatgtat aacaattttt acctttccct ttggctgagt
    20761 aaagagctat atccgcattt ctaaatagtg tcatatagtc cataccatct tgtggaaact
    20821 tagcaattcc tacacttaat gatattttat attttcgtcc tttacctaaa aaagaccttt
    20881 caaatgtttt taccagttct tctgctttat ttataatatc tcttgtttca gaaatatctt
    20941 ttataaatac aataaactca tctccaccaa ttctacctac tatatcggtt gctctaaata
    21001 gatttactag gtcacttgca atttcactta atacagcatc cccatataaa tgtcctaaat
    21061 tatcatttat tcctttaaag tcatcaatat caataatcat taaagcacct tttctttttt
    21121 tatctaatgt tatgtattct tctataagtg attgtgatgc ttttttatta tacaaatctg
    21181 taagagggtc tttttctgcc ctttctttta acttttcagc ttctcgtttt gattcatcaa
    21241 tatcagtcaa acttcctact accttataag gtttaccatt attatcaaat aaggttgttg
    21301 cctttatgct aaaccatgca taggtagtat ctctagttct aagtctaatt tcacgctctt
    21361 catattcact accttctgaa gtaagtttaa ttagtgaaat aaacttatcc atatcatcat
    21421 gatatatatc tactgattta attatatttt ttgttgcatt ttcagttata gggtcatatt
    21481 taaacttatt ataccaattg ggtgatatat tcattttgtc tttttctata tcccattcta
    21541 ttataatatt ttgcgtctgc tttagaatga tttcatggcg ttcttcactc tttttaagtt
    21601 cttgctctaa taatttagca gaagtaatat ctacaataac acaacaaagt acagattttc
    21661 cattttcata aattatcttt cttcctttta ataaaatcca tataatagta ccctcccttt
    21721 tttcaactct ttgttcagat gcaaatgtat cacttaattt gagttgtttg tttatactat
    21781 taattagccc tattctatca tctttaaaaa taagttttat taatttatta tttagctcat
    21841 ccttcagttg tttacgtgta tatccaatca tatttaaata accttcattt aaatatatac
    21901 tatcaaaatc atcatttatc tcagttatca ctatcccacc aacaatacta tcattgatgg
    21961 cctgtatttc aaatcttttt gaatctaact cttcttgtat tttcttaagt tctgttatat
    22021 cagttaaaac tccttgaata cactcacttc ctttattatc atttatagac ttgcttctct
    22081 ccaaaatcca aattatactt ccatcttttt ttacaattct atattcaact tcaattttat
    22141 cttgtttttc taattgattt agaataatac aattaaactt gtctaaatca tctttgtaaa
    22201 tcatatcttt aaatctattt ttaaatattt tatttaaatc ttcatcagag taacctgtaa
    22261 gttctaaaaa tccctgactt acatatgtca tagtcatata ttcatctttt aaacaggttt
    22321 taactcctcc tagtatatta tcagtgagta ctttaaattt ttctttttca tttataataa
    22381 ttttggtgtt attatttata atacatgcta tatatattat aaaaatcaat ataactaata
    22441 gtatttctaa tgcaaataca tatacatcat aagatatatt ctctgactga atagacacta
    22501 ttttatttgg cacaacagaa aaaacatacc aatcattaac tccaagtggt tgatagttta
    22561 acataaattt ttcattatct aagtcataat taaaatatcc agatttatca tttttcatat
    22621 tttcttttat agtttttaga caatagactt catcacaatt atttttgtta aagagctgaa
    22681 atatacacga tagtgtctta ctatttttat ttgttttttg agcaattata gctccatttt
    22741 tatttgcaac aaaaacatct gctttttcat caaatgcaga taatcctaat aattcattta
    22801 acttttttga atcataaatt ccacttaaaa caccaactat tttcccactt ttataaactg
    22861 gagtagataa aactattatt tctttattat taacaataga ttttaaaggc tctgatatat
    22921 tcctttctcc atttttagct tttttaaaat agtctctatc ttgaacatta cgaataattc
    22981 catcattaga ataacattct ccatctaatg tggagatagt cattctcatt aaagaacttc
    23041 gctttacatt agagtccatt aaatcaatca ccattttatc atttatatta ttcaagtttc
    23101 ctatgaaatt agcaatggat tcaagcatgt caaattgacc actaagtctt gtcttgagat
    23161 taattatatt gtgttctgta aactcagaca agtatgtatg tgtggtttct tttaacttat
    23221 tatctaattt aaatttaaac ttgaaaatag ctccacatgt taaaataatt ataataataa
    23281 acaagatact aatacctaaa attcttggct ttagtctgtt catcatactc acatcctcat
    23341 tgtatataat tatattactt attttgcaag cctttgtatt tatttaagtt gtttattttt
    23401 atttctatga ttttttcaat atacatttac cttcttcccg cattagaatt aaataacata
    23461 gcttaaggtt tatttgtaaa atctttgttc atatttcatc attcttaatt aatattaaaa
    23521 actctgacct tatttttgat ttatcaaaag taataatgta atatattcat tattattcat
    23581 tccttttcta attaattact cttcagttaa tttgacaaca aaaaaatact ctctaatcac
    23641 tccctctaaa aaatatcttg tctttttata tgattcagtt agcaaattaa ttttgtatac
    23701 ataaaattta agccctataa aaacatatac tttagattca cactcaaaac ttagtttacc
    23761 agataataat tctagtaaaa tattataata tccttt
//
LOCUS       DAFNNB010000044        23606 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0044, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000044 DAFNNB010000000
VERSION     DAFNNB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23606
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0044"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1682)
                     /locus_tag="L3M60_001545"
     CDS             complement(<1..1682)
                     /locus_tag="L3M60_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226223.1"
                     /translation="MDFKKFAYNNIVRNFRAYLAYFLSSVFSIMIFFVFAVSMFHPII
                     MKSGIQSGFTAFMALMTSELIILGFSLLFILYSLGNFLKYRLKDFGVLMIIGMSNRQL
                     KKLILIENLIIGFLAVMLGILLGLSISKLFLLYLSKLFYMNLTELYFPVKAILLTVVS
                     FMILFLITGPMSLKLLNKKSILELLLGTKKPKKEIKSSKLFGIAGIVCLILGYAMILF
                     GNKLKINGGMYTTTLLITLGIFLFFSQFTILILKYLKNKEKFYKNKVNMLLISNLMYK
                     LKDNTRLLFLITILLSATLVAFTTTSTLVSSQGESSKNNFPMVYSYFSEHNNEKEYEE
                     LQKIRDTIKRYDYKELSFPILQYNREVLLNVKDYNKLSKELGLDKISSKLRKYEGIII
                     PSSNSQEHINSLKELRNYSIKNLNIKIKKSTNKIIFPTGLFSKVVVISDDLYNEVKGE
                     FSKMNFHGFDYKNWQNSAKITENLKVKHFNLDREYNRSYFLTLPDMYLVELQQSKILQ
                     FMGVFIGVILFIAVFSFLYFRLYTDEMEDNVKYKKLSKIGLSFKNMNKIVSI"
     gene            complement(1682..2428)
                     /locus_tag="L3M60_001546"
     CDS             complement(1682..2428)
                     /locus_tag="L3M60_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226224.1"
                     /translation="MNILSIRNISKTYCGNIPFKALDKVSLNIDKGEFVSVMGPSGSG
                     KSTLLNIISTVDRQSEGEVILDGYDISKLEGEKLAEFRRKQLGFVFQDFNLIDTLTVG
                     ENIMLPLTLEGASIKDINIQTRNISKFLGIDKILDRKTYEISGGQAQRCAIARAIINK
                     PAILLADEPTGNLDSKSTDDVLKLFTKINIEQNVTTLMVTHEAYSASYSDRVIFIKDG
                     CIYTEIKKSESSNSFYSDILAVLSQIGGVR"
     gene            complement(2581..4800)
                     /locus_tag="L3M60_001547"
     CDS             complement(2581..4800)
                     /locus_tag="L3M60_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6226225.1"
                     /translation="MKKREKVFDGLVFKHILILLILLVLIDLTSTIIYASNSMEITSR
                     NMIETSKRELENYLEINISLLKALSKDDRFSDSETSLIEKGKLLRPYQKEYNLFMIGI
                     TDTKGNTSSTYREKVGSIKDRPSFEKAIKTKQVVVSDIEVSNVTGDKVFIIYVPIIKN
                     NEMIGTIFASFYFQDVNNLISRSNLDDSIKFLMIDKNYTIISHPNKKYVNDKSKILDL
                     EGNIIGTTKSEILKNINEKCQGKFLYWDNWRLYNVKYTNIKWTNWTLVSKCNIFKNFQ
                     SLIVNFMIKLYFYIVIFMILWKLSNAKLIEQLKKLAYYDSLSGIKNKEKFRKDSMYIL
                     KNYYQDNFYLVQLDVNKFKYINEMFGYAEGNKILIHISQVLNNNTNKYEICARMDNDH
                     FILLIACSTEDELLNRLSKINKEICNLSTTNSSKYKIVMSSGIYKINKKDDIKKIDLL
                     IDRANIAAKSKKEKYEHSYSFFNEETRNRLYKEKRLEDNMNKALEKGEFIVYYQPKYS
                     LNDVNEIEGAEALIRWNSPEFGFISPIDFVPLFEKNGFIVNIDMFVFEEVCKTLNKWI
                     NKGYTPVPISVNMSRVHLYRDNFIENITDLISKYNISPEFIELELTESVVFDNLNILI
                     DIMKKIKEIGFLISMDDFGSGYSSLNLLKDLSFDILKLDRGFLIETTDTKRGKIIISK
                     IVEMAKAIDIKVICEGVETYEQVEFLREIGCDKVQGYLFAKPMVLDEFEKHLNFKFD"
     gene            complement(5165..6559)
                     /gene="gltA"
                     /locus_tag="L3M60_001548"
     CDS             complement(5165..6559)
                     /gene="gltA"
                     /locus_tag="L3M60_001548"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HBN6226226.1"
                     /translation="MDAKKVKVPVREQEPAVRATNFDEVCLGYNKEEAMAEANRCLAC
                     KKPKCVGGCPVGIDIPGFITKIKEDDIEGAAKVIAKSSSLPAVCGRVCPQESQCEGVC
                     ILGIKSDAVSIGKLERFVADWSKENDINLSDTEPKKNQKVAVIGSGPAGLACAGDLAK
                     KGYDVTIFEAMHEPGGVLTYGIPEFRLPKQAVVQPEIDNIRKLGVKIETNVIVGKTIT
                     VDELIEDEGFEAIFIGSGAGLPMFMNIPGENANGVFSANEFLTRVNLMKAYRDDYDTP
                     ISSGKKVAVVGGGNVAMDAARTALRLGSESYIVYRRSEKELPARAEEVHHAKEEGIIF
                     NTLTNPKEILVDENGYVKGMVCIRMELGEPDDSGRRRPIEIEGSEFVLDVDTVIMSLG
                     TSPNPLISSTTKSLDINKKRCLITDENGQTSKEGVFAGGDAVTGAATVISAMGAGKTA
                     AASIDEYLKAKVNA"
     gene            complement(6559..7452)
                     /locus_tag="L3M60_001549"
     CDS             complement(6559..7452)
                     /locus_tag="L3M60_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HBN6226227.1"
                     /translation="MSNKIVSKRQLTDSIYLMEIEAPRVAKSSQPGQFIIIKNDEKGE
                     RIPLTIADYDREKGTVTIVFQTVGASTKKLAMFEENDFVMDFVGPLGQASEFIHEDIE
                     ELKNKKILFVAGGVGSAPVYPQVKWFKEHGLDVDVIIGARTKELIILEDDMKKVAKNV
                     YVSTDDGTYGFNGRVTDLLKDLVDNQGKKYDQAIVIGPMIMMKFMCQLTKELNIPTIV
                     SLNTIMIDGTGMCGGCRVSVGNETKFACVDGPEFDGHLVDFDQAMRRQSMYKTQEGRA
                     MLKLEEGDSHHHSNCGCGGNK"
     gene            8078..8929
                     /locus_tag="L3M60_001550"
     CDS             8078..8929
                     /locus_tag="L3M60_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HBN6226228.1"
                     /translation="MKNIISAKELISKLEQNDNLVVIDCRFDLINRTYGIDSYKKGHI
                     KGSFILDIDKDLSSPTKEHGGKNPLQDPLILKEKLEKMGVDNNTTIVTYDDGDLNGAC
                     RLFFQLKHLGLKNVYVLDGGITSFVKEGGELEKKVNVPSCTGKEIKPNINNDLVVPME
                     YVKSKLYKKDTVIIDCRANERYQGLVEPAYSKAGHIPSAKNYFCKDLIKSDFENGSLK
                     DIEFLKKFFKDLNNYDEVILSCGSGISACVNSLALRELDIPHKIYIGSFSDWISYDDN
                     EIKTGQE"
     gene            complement(9057..10670)
                     /gene="ggt"
                     /locus_tag="L3M60_001551"
     CDS             complement(9057..10670)
                     /gene="ggt"
                     /locus_tag="L3M60_001551"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBN6226229.1"
                     /translation="MKFNAYQHKYSSIRNVVYAKNGAVATSTPLASQAGLEILKKGGN
                     AVDAAVATAATLAVVEPTSNGIGGDAYALVWIEEEKRLYGLNSSGFAPENMELKNYNN
                     MKEMPKYGFGAVTVPGIPAAWSELNKKYGKLSLIECLSPAINYAREGYVVSPNVAKVW
                     KKSYELYEKELIGEEFKPWFDTFSKDGKAPEAGDIFICEEQASTLEEIANTQAESFYR
                     GRLADKIDEYSRKFNGAIRKSDLECFYPTWVEPISTEYKGYKIFEIPPNGHGITVLMA
                     LNILKELKLEGNIENVEDIHKIIESLKLAFADSKTYVTDIEHMKVKIQELLSQEYAKK
                     RSLLIDNKEALYPTAGEPYCGGTVYLCTADKDGNMVSYIQSNYINFGSGIVIPRTGIA
                     LHSRGNNFNLDPEHHNVVKPFKKPYHTIIPGFLGKEDKAIGPFGVMGAFMQPQGHIQV
                     LTNMIDFGLNPQEALDAPRWQWIKGKEIEVESEMPKHIIDSLIEKGHEIKVIHDTVDM
                     GRGQIIFKTEQESYICGTESRCDGHIAVY"
     gene            complement(10902..12839)
                     /locus_tag="L3M60_001552"
     CDS             complement(10902..12839)
                     /locus_tag="L3M60_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226230.1"
                     /translation="MTLFRIAMKNVKNSFFNYLMYFISIVFSVFIFFSFKSIEYNKAL
                     SSLGEKTRMSINTSSILIVAFVFLFIYYSNSFFFNRRSREVGTYSLLGMRKNQIGKIF
                     LYETFLMGIVAILIGIFLGLLFSKLMTMMLVKLMNEMIVVNMNLSIKALVQTLAVFLT
                     IFIVIGIRNTIVIRNKRIIELFNKPPEKCRFKKFIKLKGALGVLLITISYLMSISYFI
                     AENIMFSVFILVTIIPGTFLFFSSVMYMIIDAVKKRKGFYYKGQNLIAFSELGFKLKS
                     NSSVLAIIAILIATSVTILGFTISLYYDIDRNISENYKYSYTINLGNSHVNNKIDKLL
                     DKYKKDNKIIFDKTIELIDKDINLEMYYKNGNSYIEDRTSVDIIRESDFKKLRQYQNN
                     NYEELISKESVYYVSDSYKKILFKDFEIEKINLHFKDTSECDINLFTVQKTILEPEIN
                     SQATFDLIVVKDKVFNQLKLHGDSRLIRLIDIKNEKKELNLTLKLKNIVSENMEFTYP
                     FNFTSSIESYDNLIKLSGLMLFIGIFLSVVFLLCTGSIILFKQLSNIYDDRERYIMLT
                     KLGANNKDIEKIISKQLKVIFLMPLVVGTVHNLFAMSIVQKFIPRSLLVPIIITLVIY
                     FIGYFIYYFLTLKYALNMITE"
     gene            complement(12826..13593)
                     /locus_tag="L3M60_001553"
     CDS             complement(12826..13593)
                     /locus_tag="L3M60_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003722224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226231.1"
                     /translation="MEEILSVENIKKEYGRKGSKHEALRGITFKVYKGEFVGIMGSSG
                     AGKSTLLNIISTIDLPSSGDIYINGKNTIKMKQNELADFRRDNLGFVFQDSNLLDTLT
                     IKENIMLPLSLKNERVSVIENRINEISRELNIESILDKYPSEVSGGQKQRGAVCRAIA
                     TKPSLVLADEPTGALDSKSARDLLNCLLKLNKDSNKTILMVTHDAISASFCNRILFIK
                     DGIIFTEIVKGESNREFYNKIVNTVSLIGGVNKNDFI"
     gene            13881..14555
                     /locus_tag="L3M60_001554"
     CDS             13881..14555
                     /locus_tag="L3M60_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226232.1"
                     /translation="MFKIMVVEDDVSLKNIIAECLTKWGHNVHQIENLENIIEEFKNY
                     KPQLVLLDINLPFYDGFYWCNEIRKISKVPIIFISSRNSNMDVIMGVNLGADDYIQKP
                     FSIDVLVAKVNALLRRTYNFVDNNSNQIIHNGVTLDLSTATINYEDNTIELTKNEIKI
                     LHELMKYKGQIVSRNKLMKKLWDNDWFVDDNTLTVNVNRIRSKLNEIGLEDFIETKRG
                     LGYIIN"
     gene            14579..15583
                     /locus_tag="L3M60_001555"
     CDS             14579..15583
                     /locus_tag="L3M60_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6226233.1"
                     /translation="MSFKDFLKDKLGFLVYNFTFLIFTIAVIIFSPVDVFLIDTILYI
                     LVINVVFLFLYLFISYIKKNKFLDKIKNDTFKININTTELAKCKNEEKIYLNIIKNHQ
                     YQCNNIINKNIKKFEENKEILNMWVHDVKIPIAIIKLTLEQNENLIDEKISDDIDSEI
                     FRIENSVEKILYLSRLEDFHKDFLIQEVNIEKIIRNIIKKYSKYFISKKIILNLNNLN
                     FKVLSDEKWLYFIFEQLLSNSLKYSSLGDNISIYCKRSKNYLQLRVEDTGCGIKKEDL
                     NRIFNKGFTGNNGRNNSKSTGLGLYLVKELCDKLGHKIAVDSEYNQYTKFTITFKCDL
                     "
     gene            15694..16926
                     /locus_tag="L3M60_001556"
     CDS             15694..16926
                     /locus_tag="L3M60_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HBN6226234.1"
                     /translation="MSFLKKFKNKRFRNNSFKSNNFRSSSFGSSGFNSKNKGFKNNPF
                     KNKRFKIYAGIAVIILLILGVLAYVDSKNTKLQANENFIETYTIPENEKIFINGMVVP
                     KQTKDFNISGDYELSDVNVTNGQKVNQGDLLFTAKNPTIIAEIDSLKSQLSQYKKQKI
                     SLSDIAENRDAIASINAQITTLNSQIASLDKKAYDRVTAPFDGTVYLNDQTGNPDQPV
                     SFMTIQGLEFYMKGQASEQDLPKMKIDQMVNILVFSTDQKLTGRISFISDKPSTPNTE
                     MGAQQNTLSYYDINIAFDNQEGLVNGFHLQASLEVSNSSFKIPASCVLKDKKHSYVFK
                     DLDGILKKQIVDVASQNDDFAVVRGGLEQGDIIIKHPTKEMKEGDPVQGDGVTAGSNN
                     NGDTTPNKKVEEVNMDVN"
     gene            16934..17608
                     /locus_tag="L3M60_001557"
     CDS             16934..17608
                     /locus_tag="L3M60_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226235.1"
                     /translation="MLIKLENIQKYYKVGKDELHVLKSLNLEIESGEFVMIMGKSGSG
                     KTTLLNILGFLDVFDEGRYIFDGTDVTNLSENERSVFRNINIGFVFQQFNLIETLNVY
                     QNVELPLIYNKSLKKSNREEIVKDKLSSVGLLDKLKQKPLQLSGGQQQRVAIARCLAN
                     DPQIIFADEPTGALDSETSREIMELLTRLNKQGKTIIMVTHDQDLTKYATKVIRLKDG
                     VFTSEV"
     gene            17611..18843
                     /locus_tag="L3M60_001558"
     CDS             17611..18843
                     /locus_tag="L3M60_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226236.1"
                     /translation="MSLLKNSLANLKGHKLRVFVALLWIIIGITSVILVSSIGNGFQK
                     EIKKSVNNVNPNKTTISFESADNTGLTDDMSIFLKPFNAKDLEELSFVEGVERIAPSR
                     DGFNLDSVYSSQASFDKKTTYVDVGPVKKDSKINLICGRDFSLDDEKRKVILLTLQST
                     SEIFENPEDALGHGININGTIFEIIGVLDDSQQNQAGGFFGGYQDMQFTTSLIPKKAF
                     DTLMSQNSYSNEIYQLDLVSSKGYNVNEVANNVISKLYEMHPGINGSYTTPDPTEQTA
                     YLESINSNVNKYVSIITVVAMFVGGIGVMNIMYVSVMERQREIGIRRAIGAKPRSILF
                     QFLVEAVFITVCGGILGTIVGFAATNYVSKYIGFEAIPSLNSLFYAIIATILTGVVFG
                     LIPAFKASKLDPIKAIYK"
     gene            complement(19128..20393)
                     /locus_tag="L3M60_001559"
     CDS             complement(19128..20393)
                     /locus_tag="L3M60_001559"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBN6226237.1"
                     /translation="MILIKNGRVIDPKSQRDENIDLIIKENKIYKIGKFDESDEYEKI
                     IDASGNVVAPGLVDVHVHFRDPGFTYKEDIESGAKSAARGGFTTVICMANTNPIVDNE
                     DTFNYVKEKSKNACINVLQAAAITKGFEGKELVDMEALKKAGVPGFTDDGLPLMDSNL
                     IMEAMVKAKELDVPLSFHEEDPSLVGNPGVNAGKIASELGLKGASSVAEDVMVARDCM
                     LALKTGAKVDIQHISSGVSVDMVRFAKYLGANVVAEASPHHFTLTEEDVLEFGTNAKM
                     NPPLRSKWDRDKIIEGLNDGTIEIIATDHAPHSREEKDREFIKAPSGIIGLETSLALG
                     ITNLVHKNHLSMMQLIEKMSTNPAKLYNLNTGFIEEGAVADIVIFNPEEEWTVESFVS
                     KADNSPFKGKSLYGKINYTICNGKIVYNA"
     gene            complement(20446..21594)
                     /locus_tag="L3M60_001560"
     CDS             complement(20446..21594)
                     /locus_tag="L3M60_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HBN6226238.1"
                     /translation="MKSVSVRSCKTYNYEEVRLSIEKNLEDIGGIDKFVKKGSKVLIK
                     PNLLMKKKPEEATTTHPMVVKVVCEKLLELNCDITIADSPGGPYNQSSLKSIYKASGI
                     EEVANELGIKLNYDVSDVKVHSEKAHVLKYMDIIKPVVDSDYIINLCKLKTHIMATFT
                     GGTKNLYGCIAGLKKAEIHYRFPTEELFCENVLLDICDYIKPTLTIMDGIVGMEGDGP
                     SAGTTREIGVLLASQNPYAIDVVACKIISLIPNRVATIRGAIKRGYIREDFSDIEILG
                     EDINDLIIKNYKVPNVSKDLRLFSVKLPKFLNEPISKLITPKPVVRFQDCIKCGKCKE
                     ACPAATIEIGTKGAIIDLSKCIRCYCCHELCPKKAIDVKRNFVFKYVK"
     gene            complement(21685..22230)
                     /locus_tag="L3M60_001561"
     CDS             complement(21685..22230)
                     /locus_tag="L3M60_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBN6226239.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEMVWADE
                     HRIGVAQGVDAEIIEGLRANFTGECTEVGMYLAMSRQADREGYPEVAEAYKRIAFEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKRAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(22453..23397)
                     /locus_tag="L3M60_001562"
     CDS             complement(22453..23397)
                     /locus_tag="L3M60_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN6226240.1"
                     /translation="MQKNKKEGALLKKYFGEIGLIFIAIIWGSGFVATQFALDGGLTP
                     LQIITLRFFLAAIIMNLLFFKQIRANMGKKLLKAGGILGIFLFLAFTVQTIGLMYTTP
                     SKNAFITAANVVIVPFIGFILYRRKLDKIGIISSLVALIGIGILSLEADFSINFGDFL
                     TLICSFGFAFHIFFTSEFAKDNNPMALTAIQFTVAFLMSVIVQAFAGQLKMEAELSGY
                     MGTMYLAVFSTTIGFLFQTICQKRVDGTRTAIILSTEAVFGTIFSIIILKELITAKLV
                     IGSILIFVAIITAETKLSFLKSKKVKLKDSEESSLESI"
BASE COUNT         8140 a         3844 c         2698 g         8924 t
ORIGIN      
        1 tctatactaa caatcttatt catattttta aaagatagac ctatttttga tagtttttta
       61 tattttacat tatcttccat ctcatctgta tataatctga aatataaaaa actaaaaact
      121 gctataaata atatgactcc tataaaaaca cccataaatt gaagaatttt actttgttgt
      181 aactctacta aatacatatc tggtaatgtt aaaaaatatg acctgttata ttctctatcc
      241 aaattaaaat gttttacttt taaattttca gttatctttg cactgttttg ccagttttta
      301 taatcgaatc catgaaaatt catttttgaa aattcaccct ttacttcatt gtataaatcg
      361 tcacttataa ctactacttt actgaacaat cctgtaggaa aaattatttt atttgttgat
      421 ttttttattt ttatatttaa attttttata ctataatttc ttaattcttt taaagagttt
      481 atatgttctt gtgaattgct actaggaatt ataatacctt catactttct taactttgaa
      541 ctaattttat ctaatcccaa ttcctttgat agtttgttat aatcctttac atttagtaag
      601 acttccctat tatattgaag tataggaaaa cttaactcct tataatcata tcttttgatt
      661 gtatctctga ttttttgtag ttcttcatat tctttttcat tattatgctc tgaaaaatat
      721 gagtaaacca ttggaaaatt atttttagaa gattctcctt gagaacttac aagtgttgaa
      781 gttgttgtaa atgctactaa agtggctgat aaaagaattg ttattaaaaa taacaatcgt
      841 gtattatcct ttaatttata catcaaatta cttataagta acatatttac tttgttctta
      901 taaaattttt ccttattttt taaatacttt aatatcaaaa tcgtaaattg agaaaaaaat
      961 aaaaatatcc ccaaagtaat taacagtgtt gttgtataca tacctccatt tatttttaac
     1021 ttattgccaa atagtatcat agcatatcct aatataagac atactatacc agctattcca
     1081 aataactttg aagattttat ctctttttta ggcttttttg tacctagtaa aagctctaaa
     1141 atactctttt tatttaataa ttttaagctc attggtcctg ttattaaaaa caaaatcata
     1201 aatgatacaa cagtcaatag tattgctttt accggaaaat aaagttcagt taaattcata
     1261 taaaacagtt tgcttaaata caataaaaac aatttggaaa ttgataaacc aagtaatatt
     1321 cctaacatca ctgctaaaaa ccctattatc aaattttcaa ttaatatcaa ttttttcagt
     1381 tgtctgttac tcattccaat tatcatcaat actccaaaat ctttcaatct atattttaaa
     1441 aagtttccta aagaatacaa tataaaaagt agactaaaac ctaagattat taattctgaa
     1501 gtcataagag ccataaatgc agtaaagcca ctttggattc cagatttcat tataattgga
     1561 tgaaacatag atactgcaaa gacaaaaaat atcattatag aaaaaaccga acttaaaaaa
     1621 tatgctaaat atgctctaaa atttctaact atattattat aagcaaattt tttaaaatcc
     1681 actatcttac acctcctatt tgagacaata cagctagtat atcagaatag aaagaattac
     1741 tgctttcaga tttttttatt tctgtatata tacaaccatc ttttataaaa atcactctat
     1801 ctgaataact tgcactatat gcttcatgag ttaccataag tgttgttaca ttttgttcta
     1861 tgtttatctt agtaaataat ttcagaacat catcagtaga ttttgagtca agatttccag
     1921 ttggttcatc tgcaagaagt atggcaggtt tatttataat ggctctagca atagcacacc
     1981 tttgagcttg ccctcctgaa atttcatagg tttttctatc taaaatttta tctattccta
     2041 aaaatttaga gatatttcta gtttgtatat taatatcttt aatacttgcc ccttctagtg
     2101 taagtggcaa cataatattt tcgccaacag ttaaagtatc aatcaaattg aaatcctgaa
     2161 atacaaaacc aagttgtttc cttctaaatt cagcaagttt ttctccttct aatttactta
     2221 tatcatatcc atctaatata acctcacctt cactctgtct atcaactgta gatattatat
     2281 ttaaaagagt agatttgcca cttccagatg gtcccataac agacacaaat tctcctttat
     2341 ctatatttaa actaacctta tctaatgctt taaatggaat atttccacaa tatgtttttg
     2401 atatatttct aatactcaat atgttcattt tatattctcc ataacttttt atttttacta
     2461 caataccata atatcgtgaa tatactattt taaccataaa aataccctaa caattaactt
     2521 acatatttgt aagtaagttg ttagagtatc taatagtata ataattatta atgattatga
     2581 ttaatcaaat ttaaagttta agtgtttttc aaattcatct aataccatag gcttagcaaa
     2641 taagtatcct tgaaccttat cacaacctat ctctcttaag aactctactt gttcataagt
     2701 ttctacacct tcacagatta ctttaatatc aatcgctttt gccatctcca caatcttaga
     2761 aataataatt tttccacgtt ttgtatctgt tgtttcgatt aaaaatcctc tatccaattt
     2821 taaaatgtca aaagaaaggt cttttaacag atttaaagat gaatatcctg aaccaaaatc
     2881 atccattgat ataagaaatc ctatttcttt aatctttttc attatatcaa tcaatatatt
     2941 taaattatca aacacaacgc tttctgttag ttcaagttca ataaactcag gagatatatt
     3001 gtatttactt atcaaatcag ttatattttc tataaaattg tccctatata aatgaactct
     3061 agacatattt acagaaattg gtacaggagt ataaccttta tttatccact tatttaatgt
     3121 tttgcatact tcttcaaaca cgaacatatc aatatttaca ataaaaccat tcttttcaaa
     3181 taaaggtaca aaatctatag gtgaaataaa cccaaattct ggactattcc atcttatcaa
     3241 agcttctgct ccttcaattt catttacatc atttaaacta tacttaggtt ggtaatacac
     3301 tataaattca cctttttcta aagctttatt catattatct tccaatcgtt tttccttata
     3361 caatcgattt cttgtttctt cattaaaaaa tgaatatgag tgttcatatt tttccttttt
     3421 gcttttggct gcaatatttg ctctatcaat taacaggtca atctttttaa tatcatcctt
     3481 tttattaatc ttatatattc cacttgacat aacaatttta tacttagatg agttagtagt
     3541 acttaaatta caaatttctt tatttatttt agataatcta tttaaaagtt catcttcagt
     3601 gctacaagca atcaacaata taaaatggtc attatccatc ctagcacaaa tttcatattt
     3661 atttgtatta ttatttaaaa cctgtgagat atgtattaat atcttatttc cttcagcata
     3721 gccaaacatt tcatttatgt atttaaattt attgacatct aattgtacta agtaaaaatt
     3781 atcctgataa taattcttta aaatatacat agaatctttt ctaaactttt ctttattttt
     3841 tattccactt aaagaatcat aataagccaa ttttttaagt tgttctatta acttagcgtt
     3901 acttaatttc cacaatatca taaatattac tatataaaaa tacaacttta tcataaaatt
     3961 aactattaga ctttgaaagt tcttaaatat attacatttt gatactaaag tccaattggt
     4021 ccactttatg ttcgtatatt ttacattata taatctccaa ttatcccaat ataaaaattt
     4081 accttgacac ttctcattta tattttttaa aatttcactt ttagtagtcc ctataatatt
     4141 tccttctaaa tcaagaatct tacttttatc atttacatat tttttgtttg gatgagaaat
     4201 aatagtataa tttttatcta tcattagaaa tttaatagaa tcatcaagat tgcttctact
     4261 tattaaatta tttacgtctt gaaaataaaa agaagcaaaa atagttccaa tcatctcatt
     4321 attttttata attggtacat atataataaa taccttatca cctgtcacat tgctaacttc
     4381 aatatctgag actactactt gctttgtttt aattgctttt tcaaaactag gcctatcctt
     4441 aattgagcca accttttccc tatatgtact tgaagtattt ccttttgtat ctgttatacc
     4501 aatcataaaa agattatatt ctttttgata tggtcttaat aattttccct tttcaattag
     4561 agatgtttca ctatcagaaa atctatcatc tttactaaga gcttttaata aagatatgtt
     4621 tatttctaaa tagttttcaa gttctctttt tgatgtctct atcatatttc tagaagtaat
     4681 ttccatagaa ttacttgcat atatgatggt gcttgttaaa tcaatcaaaa ctaataatat
     4741 tagtaaaatt aatatatgct taaaaactaa tccatcaaat actttttctc tttttttcat
     4801 attaaaatac cattaattat acaatttatt tttctattat ctaatgctag atttgacaaa
     4861 tctgatttta ttagccacac attattcact ccttatagac ctttattgta taactatttt
     4921 atcatagtta tacagagtta tgttacttag tattaaatat tacaataata acttataaat
     4981 tattaaacaa tctgaaaaag tggatacatc aaaatagaga ttgtgctaag acctaaaggt
     5041 tagactttat acaggaatga aattttcatc gttgaaatga ttaaaatata tttgattttg
     5101 tacaaaaaga gtgcctcatg gactacaaag ttcattttga gacaccctct ctttaattta
     5161 caaattaagc attaactttt gcttttaaat attcatctat tgatgctgct gctgttttac
     5221 cagcacccat tgcagatatt actgttgctg ctccagtaac agcatctcca cctgcaaaca
     5281 caccttcttt agaagtttga ccgttttcat ctgttattaa acatctcttc ttatttatat
     5341 caagactctt tgttgtagaa gatattagtg gatttggact tgttccaagt gacattataa
     5401 ctgtatctac atcaagtaca aattcagaac cttctatttc tataggtctt cttcttccag
     5461 aatcatctgg ctctcctaat tccattctta tacaaaccat acctttaaca tatccatttt
     5521 catccactaa aatttcctta ggatttgtta gtgtattaaa tataatacct tcttcttttg
     5581 catgatgaac ttcttcagct cttgctggga gctctttttc acttcttcta taaactatat
     5641 atgattctga acccaatctt agtgcagttc tagcagcgtc catagcaaca tttccaccac
     5701 ctactacagc aactttttta ccagaactaa taggtgtatc atagtcatct ctataagcct
     5761 tcattaagtt tactctagtt aaaaactcat ttgcagaaaa tactccattt gcattttctc
     5821 ctggtatatt cataaacatt ggaagaccag ctcctgaacc tatgaatata gcttcaaatc
     5881 cttcatcttc tataagttca tctactgtaa ttgttttacc aacaattaca ttagtctcta
     5941 tttttacacc taattttctt atattatcta tttcaggttg aacaacagct tgttttggaa
     6001 gtctaaattc aggtattcca taagttaaaa ctccacctgg ctcatgcatt gcttcaaaga
     6061 ttgttacatc atagcctttt ttagctaagt caccagcaca agctagacct gctggaccac
     6121 ttcctattac tgctaccttt tgatttttct taggctctgt atcagataaa tttatgtcat
     6181 tttcttttga ccaatctgct acaaatcttt ctaacttacc tattgataca gcatctgatt
     6241 ttattccaag tatacataca ccttcacatt ggctttcttg tggacacact ctaccacaaa
     6301 cggctggtaa tgagctactc ttagctatta ctttagcagc accttctata tcgtcttctt
     6361 taatttttgt tataaaccct ggtatgtcga ttcctactgg acatccacct acacatttag
     6421 gtttcttaca agctaaacat ctattagctt ctgccatagc ttcttcttta ttatacccta
     6481 aacatacttc atcaaaattt gttgctctaa cagcaggttc ttgctctcta actggtactt
     6541 ttactttttt agcatccatt atttgttacc tccacatcca caattactat gatgatgact
     6601 atcgccttct tcaagtttta gcatagctct tccctcttga gtcttgtaca tagattgtct
     6661 tctcattgct tgatcaaaat caactaaatg accatcaaat tcaggaccat ctacacatgc
     6721 aaacttagtt tcatttccaa cgctaactct acaaccacca cacatacctg ttccatctat
     6781 cattattgta tttaaactta ctatagtagg tatatttagt tctttagtca attggcacat
     6841 aaatttcatc attatcattg gtccaattac tattgcttgg tcgtattttt taccttgatt
     6901 atcaactaaa tcttttaata ggtctgtaac tcttccatta aatccatatg taccatcatc
     6961 tgttgataca tatacatttt tagctacttt tttcatatca tcttctaata taattaattc
     7021 tttagttctt gcaccaatta ttacatctac atctaaacca tgctctttaa accatttaac
     7081 ttgtggataa actggagcag aaccaactcc accagctaca aataatatct ttttattttt
     7141 taattcttct atatcttcat gtataaattc acttgcttgt cctagtggtc ctacaaagtc
     7201 cattacaaaa tcgttttctt caaacattgc taacttttta gttgaagcac caactgtttg
     7261 gaatacaata gttactgttc ctttttctct atcatagtca gctattgtaa gaggaatcct
     7321 ctctcctttt tcgtcatttt taattattat aaattgacca ggttgagaag attttgcaac
     7381 tctaggtgcc tctatttcca ttaaataaat actatctgtt aattgtctct tactaactat
     7441 tttattactc atctcgctat tctccattct aacaaatata tttttcccac tttaaattta
     7501 attaaaaaaa taaacacaca aaactataac tttttataat ataatattag ttcttggttg
     7561 attgttttgt attggacatc ccccctatag taattataac tgcccaaact acaaatagtt
     7621 gatattaaca aatttcttgt aaatgccaat aaacactcag tctatatcat acgtctaatt
     7681 tgttatagat ttaacctaat acacacttaa agtatactat attatgccaa atttgtaaca
     7741 aaaattttat aaatacataa taaatttttt ttattcaatt atttatttaa taagttaact
     7801 taggttttca taataacact aatttcttta aaaactaaac gtgtataaga ctttaatcta
     7861 ttgcaatacc agctttcata ttatataaaa cacactctta ttttattata tatctaaaat
     7921 tttcaatatt cattaattat ttgaattttt atataaaatt tttacaattt ctaaagattt
     7981 atttacttat gtaataaatt ataataatat tagtttataa agttaataat taatataaat
     8041 taaaataaaa ataatataaa cataggaggt acattttttg aaaaacatta tttctgcaaa
     8101 agaacttata tcaaaactag agcaaaatga taatctagtt gtaatagatt gtagatttga
     8161 ccttattaat agaacttatg gtattgattc ttataaaaaa ggccatataa aaggtagttt
     8221 catcttagat atagacaagg atttatcatc tcctacaaaa gaacatggtg gtaaaaatcc
     8281 tttacaagac cctttaatac taaaagaaaa attagaaaaa atgggtgttg ataacaacac
     8341 tacgattgta acttatgatg atggagattt aaatggagct tgcagattat ttttccaatt
     8401 aaaacatttg ggtcttaaaa atgtctatgt actagacgga ggaataacat ctttcgtaaa
     8461 agaaggtgga gaattggaaa agaaagtaaa tgtaccaagt tgtacaggta aggaaattaa
     8521 accaaatata aataatgatt tagttgtccc aatggagtat gttaaatcta aattatacaa
     8581 aaaagatact gtaataatag attgccgtgc taatgaaaga taccaaggtt tagttgaacc
     8641 tgcttactca aaagctggtc atataccaag tgcaaaaaat tatttttgta aggatttaat
     8701 aaaaagtgac tttgaaaatg gctcacttaa agatatagaa tttttaaaaa aattctttaa
     8761 agatttaaat aattatgatg aagtaatttt atcttgtggt tcagggatat ctgcttgtgt
     8821 taacagtctt gcccttagag aacttgatat acctcataag atttatattg gtagctttag
     8881 tgactggatt agttatgatg acaatgaaat caaaactggc caagaataag gctaacaata
     8941 tataaaatca taggatagct taaattttgt aaaggcgtca gtcattgtag actttcgcct
     9001 ttaattatta tttcatatac tctttaaatt tattatatat attttagttg ataatattaa
     9061 tagactgcaa tatgaccatc acatcttgac tctgtcccac agatataact ttcctgttct
     9121 gttttaaata ttatttgacc tcgtcccatg tcgacagtat catgaatcac ttttatttca
     9181 tgaccctttt caattaatga atctattata tgctttggca tctctgattc aacttctatt
     9241 tcctttcctt ttatccactg ccatcttgga gcatctaacg cttcttgtgg atttaatcca
     9301 aaatctatca tatttgttaa tacttgaata tgaccttgtg gttgcataaa agctcccatt
     9361 acaccaaatg gtcctattgc tttatcttct ttacctaaaa atccaggtat tatagtatga
     9421 taaggttttt taaatggttt tactacatta tggtgttcag ggtctaaatt aaaattatta
     9481 cctctactat gtaaagcaat cccagttctt ggtatcacaa ttcctgaacc aaaatttatg
     9541 taattactct gtatatatga aaccatattt ccatccttat ctgcagtgca aaggtatact
     9601 gtaccaccac aatatggctc tcctgcagtt ggatacagag cctctttatt atcaattaat
     9661 agacttcttt ttttggcata ctcttggctt agtaattcct gaatctttac cttcatgtgc
     9721 tctatatcag ttacatatgt tttagaatct gcaaaagcta gctttaaact ttctataatc
     9781 ttatgtatgt cttctacatt ttctatattt ccttctagtt tcagttcttt taatatatta
     9841 agtgccatta aaactgttat tccatgccca tttggtggga tttcaaatat tttatatccc
     9901 ttatattctg tacttatcgg ttctacccat gttggataaa aacactctaa gtcactcttt
     9961 cttatagccc cattaaattt tctagaatat tcatctattt tatcagcaag tcttcctcta
    10021 taaaaacttt cagcttgagt atttgctatc tcttctaatg tacttgcctg ttcttcacat
    10081 ataaatatat ctccagcctc tggagcttta ccatctttag aaaatgtatc aaaccatggc
    10141 ttgaactctt ctccaataag ttctttctca tatagttcat atgatttttt ccatacttta
    10201 gctacattag gagatactac atagccctct cttgcatagt ttatcgctgg agataaacat
    10261 tctattaagc taagcttacc atatttttta tttaattctg accaagctgc gggtatacca
    10321 ggcacagtta ctgctccaaa cccatacttt ggcatttcct tcatattatt ataatttttt
    10381 aattccatat tttctggagc aaagccacta gaatttaaac catacaatcg tttttcttcc
    10441 tctatccaaa caagggcata tgcatctcct ccaatcccat tacttgtagg ctctactact
    10501 gctaatgtag cagctgttgc aactgctgca tcaacagcat ttccaccctt ttttagtatt
    10561 tcaagtccag cttgagatgc taatggtgtt gatgttgcaa ctgctccatt tttagcatac
    10621 acgacatttc ttattgatga gtatttatgt tggtaggcat taaacttcat aaatttcagc
    10681 tcctcgtcat tttttatttg atattctgct taaatttatt atataacatc aagtaatata
    10741 actgatacaa aatgatataa aatatagact ttttcctaac cctaatttta tcattaaata
    10801 aatatcttaa gctcaattct agtattttct atttgcactt aaataaacta ttttattcaa
    10861 aacatacatc taattatttt aacttaaaaa taatttatta tttattctgt tatcatgttt
    10921 agagcatatt ttaaagttaa aaaataatat ataaaatatc ctataaaata aatgactaga
    10981 gttatgataa ttggtacaag taatgacctt ggtataaact tttgtactat agacatagca
    11041 aataaattat gaactgtacc tacaactaat ggcattaaaa atatgacttt taactgtttt
    11101 gatattattt tctcaatatc tttattattt gctccaagtt ttgttaacat aatatatctt
    11161 tccctatcat catatatatt tgataattgt ttaaacaaaa ttatactacc tgtgcataaa
    11221 agaaatacta cagataaaaa tatacctata aacagcatta gaccacttaa ttttatcaga
    11281 ttgtcataac tttcaatact ggatgtaaaa ttaaaaggat aagtaaattc catattttca
    11341 cttactatat tttttaattt taaagtcaaa tttagttctt ttttttcatt ttttatatca
    11401 attaatctta ttaacctaga atctccatgt aattttaatt gattaaatac tttatctttt
    11461 actacaatta aatcaaacgt agcttgtgaa tttatttctg gctctaagat agttttttgt
    11521 actgtaaaca gatttatatc acactcactt gtatctttaa aatgtaagtt tattttttca
    11581 atctcaaagt ctttaaaaag tattttttta tatgaatcag aaacataata cacagattcc
    11641 tttgaaatta attcttcata attgttattc tgatattgtc ttaatttttt aaaatcactt
    11701 tctctgataa tatctacaga agttctatct tctatataac tatttccatt tttatagtac
    11761 atttcaagat ttatatcttt gtctataagt tctattgttt tatcaaagat tatcttattg
    11821 tcttttttat atttatctaa taatttgtca attttgttat ttacatgaga atttcctaaa
    11881 tttatagtat aactgtattt ataattttca cttatattcc tatctatgtc gtaataaagc
    11941 gatatggtaa atcctaatat agttacactt gtagctatta atattgctat tatagccaac
    12001 acactactat tgctcttcaa tttaaatcca agttcagaaa atgcaattaa attctgtcct
    12061 ttataataaa aaccttttct cttcttaaca gcatcaataa tcatatacat cactgacgaa
    12121 aaaaataaaa aagttccagg tattattgta actaatataa aaactgaaaa cataatattt
    12181 tcagcaataa aataactaat tgacattaga taagaaatag ttattaataa aacaccaagt
    12241 gcccctttta actttataaa ttttttaaac ctacactttt ctggtggttt attaaataac
    12301 tctataatcc tcttattcct gataactata gtatttctta ttccaataac tataaatatt
    12361 gttaaaaaca cagctagtgt ctgaactaaa gcctttatac ttaagttcat atttactact
    12421 atcatttcat tcattaattt tacaagcatc atagtcataa gctttgaaaa taacaatcct
    12481 aaaaatatac ctattagtat tgcaactatc cccatcaaaa aagtttcata taaaaatatt
    12541 tttcctatct gattttttct cattccaagt aaactataag tacctacttc tcgactacgt
    12601 ctattaaaaa aaaatgagtt tgaataatat ataaataaaa atacaaatgc cactattaaa
    12661 atcgaactgg tattaatgct catcctagtt ttctctccaa gtgaactcaa tgctttgtta
    12721 tactctatag atttaaaact aaaaaatata aacacactaa atacaattga aataaagtac
    12781 attaaataat taaaaaaact attcttcaca tttttcattg caattctaaa taaagtcatt
    12841 tttgttaact cctccaatta aggaaactgt atttactatt ttattataga actctctgtt
    12901 actttctcct tttacaattt ctgtaaatat tattccatct tttataaata gtatcctatt
    12961 gcaaaaactc gcacttatag catcatgagt taccatcaat attgttttat tactatcttt
    13021 gtttaatttc aatagacagt tcaacaaatc tctagcagat ttagaatcta atgctcctgt
    13081 cggttcatct gcaagtacta atgatggttt tgttgctata gctctacata cagctcctct
    13141 ttgcttttgt ccaccagaaa cttcactagg gtatttatct aatatagatt ctatatttaa
    13201 ttctcttgaa atctcattta ttctattttc tattactgat actctttcat tttttaaaga
    13261 aagaggcaac attatatttt cttttattgt aagtgtatcc aaaagattag aatcttgaaa
    13321 tacaaatcct agattatcac gtctaaaatc cgccaattca ttttgcttca ttttaatagt
    13381 attctttcca tttatataaa tatctcctga tgaaggtaag tctattgtag aaataatatt
    13441 taaaagtgtt gatttaccag cgccagatga ccccataata ccaacaaatt ctcctttata
    13501 aaccttgaaa gttattcctc ttagagcttc atgttttgaa ccttttcttc catactcctt
    13561 ttttatattt tcaacagata atatttcttc cataattcat aatcaccttt catcataatt
    13621 agttatttgt caattctcac aaatatatta tatatattaa aatagctttt aaacatttaa
    13681 ttatattaat ttaaggttac attttcgtaa gtattaatta tatttcttca aatttattgt
    13741 aattatgttt aaaatataat ttatcaagta aaatatagtt atattaatat aattatttta
    13801 gataataagg tagaaatatt ttagtttatc aagtaaaata tagttatgtt aatataacta
    13861 ttttaaattg ggaggtaaga atgtttaaaa taatggtagt tgaagatgat gtctcattga
    13921 aaaatatcat agcagaatgt cttactaaat ggggacataa tgttcatcaa atagaaaatc
    13981 ttgaaaatat aatagaagag ttcaaaaact ataaaccaca gttagtttta ttggacataa
    14041 accttccatt ttatgatggt ttttattggt gtaatgaaat aagaaaaatt tcaaaagttc
    14101 ctataatctt tatttcttca agaaattcta atatggatgt aataatgggt gtaaatctag
    14161 gtgcagatga ttatatacaa aaaccttttt ctatagatgt cttagtggct aaagttaatg
    14221 cccttttaag aagaacttat aattttgttg ataataactc aaatcagatt attcacaatg
    14281 gtgttacttt agatttatca actgcaacaa taaactatga agataatacc attgagctaa
    14341 caaaaaatga aatcaaaatt ctccatgagc ttatgaaata taaaggtcag atagtaagta
    14401 gaaataaact catgaaaaag ttatgggata atgattggtt tgtagatgat aatacattaa
    14461 ctgtcaacgt aaatagaata cgctcaaaat taaatgaaat tggtctagaa gattttatag
    14521 aaacaaaaag aggattgggt tatataatca attaaaacga ttctaagtag gtgaatttat
    14581 gagctttaaa gattttctca aagataagtt aggtttttta gtatacaatt ttactttctt
    14641 aatttttaca atagcagtta ttatattttc tcctgtagat gtttttctga ttgatactat
    14701 tttatatata ttagttataa acgttgtgtt tttatttctc tatctattta ttagctacat
    14761 caagaagaat aaatttttag ataagataaa aaatgataca ttcaaaatta atataaatac
    14821 tacagaatta gctaaatgta aaaatgaaga aaaaatatat ttgaatatta ttaaaaatca
    14881 tcaatatcaa tgtaataaca taataaacaa gaatattaag aagtttgaag aaaacaaaga
    14941 gattttaaat atgtgggtac atgatgtaaa aattcctata gctataataa aactcaccct
    15001 cgaacaaaat gaaaatttaa ttgatgaaaa aatttcagat gacatagata gtgaaatatt
    15061 tagaattgaa aactcagttg aaaaaatttt atatttatcg agacttgaag attttcataa
    15121 ggatttttta attcaagagg taaatattga aaaaataatt agaaatatta taaaaaaata
    15181 ctcaaaatat tttatttcaa aaaaaattat tttaaactta aataatttga attttaaagt
    15241 tttatctgac gaaaaatggc tctacttcat attcgagcaa ttattgagta attccctaaa
    15301 atactcctca ttaggagata atatatctat ctattgcaaa agaagcaaaa attatttaca
    15361 attaagggtg gaagatactg gctgtggtat aaaaaaggaa gatttaaatc gaatattcaa
    15421 taagggattt acaggtaata atggaagaaa caattctaaa tctacaggac ttgggcttta
    15481 tttagtaaaa gaactttgtg ataagcttgg ccataaaatt gctgtagatt cagagtacaa
    15541 ccaatataca aaatttacta ttacattcaa atgtgattta tagtatctta ttattgatag
    15601 tttattttca ttaacttata ttcaacttct tatagtaatt ctatatgtac atcccagtat
    15661 ataataagta gtttttataa aaaggaggaa gacatgtctt ttctaaaaaa atttaaaaat
    15721 aaaagattta gaaataacag ttttaaaagt aacaacttta gaagtagcag ttttggaagt
    15781 agtggcttca attctaagaa taagggtttt aaaaacaatc cttttaaaaa taagagattc
    15841 aaaatatatg caggaatagc tgtaatcata cttctaattc taggagttct tgcttatgtt
    15901 gattcaaaaa acactaaatt acaagctaat gaaaacttta tcgaaacata tactattcct
    15961 gaaaatgaaa aaatatttat aaatgggatg gtcgttccta agcaaactaa ggatttcaat
    16021 atatctggag attatgagtt aagtgatgtt aatgttacaa atggtcaaaa agtaaatcaa
    16081 ggagaccttc tttttactgc taaaaatcca actataatag cagaaataga tagtttaaaa
    16141 tcacaactta gtcaatacaa aaaacaaaaa atatcacttt ctgatattgc tgaaaatagg
    16201 gatgctattg cctctataaa tgcacagata actactttaa actctcagat agcttctctg
    16261 gataaaaaag cgtatgacag agttactgca ccttttgatg gtacagtata cttgaatgac
    16321 caaactggaa acccagacca accagtctca tttatgacta tccaaggtct agagttttat
    16381 atgaaaggac aagcaagtga acaagattta cctaaaatga agattgacca aatggtcaat
    16441 atattagttt tttcaactga ccaaaagctt actggtagaa taagttttat atctgataaa
    16501 ccatctactc caaacactga aatgggagct cagcagaata cactgtctta ttatgatata
    16561 aacatagcat ttgataatca agaaggatta gtaaatggat tccatcttca agcatcactt
    16621 gaagtatcta attcaagctt taaaatacca gcatcttgtg tgcttaagga caaaaaacac
    16681 tcatatgtat ttaaggattt agatggtata ttgaaaaagc aaattgtaga tgtagcaagt
    16741 caaaatgatg attttgctgt agttagaggc ggtcttgaac aaggcgatat aataataaaa
    16801 caccctacta aagaaatgaa agaaggagac ccagttcaag gtgatggcgt aactgctggt
    16861 tctaataata atggagatac tactccaaac aaaaaagtag aagaagttaa tatggatgtg
    16921 aattaattta catatgttaa ttaaattaga aaatattcaa aaatattaca aagtagggaa
    16981 agatgaatta catgttttaa aatccttaaa tttagaaatt gaatctggag aatttgtcat
    17041 gataatgggt aaatctggta gtggtaaaac tactttactt aatatcttag gttttctaga
    17101 tgtatttgat gaaggaagat atatatttga tggaacagat gtaactaatt tgagtgaaaa
    17161 tgaacgttct gtttttagaa atattaatat tggatttgtt ttccaacaat ttaatttaat
    17221 agaaactcta aatgtatatc aaaatgtaga gttgcctcta atttacaata aatcgctaaa
    17281 aaaatctaat agagaagaaa ttgttaaaga taaattaagt tctgttggac ttttggataa
    17341 gcttaaacaa aaaccgcttc aattatctgg tggtcaacaa cagcgtgtag ccattgcacg
    17401 ttgcttagca aatgaccctc aaattatctt tgcagatgaa ccgactgggg cattagatag
    17461 tgaaactagc agagaaatta tggagctttt aacaaggctc aacaaacaag gtaagactat
    17521 aataatggtt acacatgatc aagatttaac taagtatgca actaaggtta tacgacttaa
    17581 agatggtgtg tttacttcgg aggtgtaatc atgagtttat taaaaaattc tttggccaat
    17641 ttaaaaggtc acaagcttcg tgtatttgta gctttactct ggattataat aggaataact
    17701 tcagtgatac ttgttagttc tataggtaat ggatttcaaa aagaaattaa aaaatctgta
    17761 aacaatgtaa accctaataa aactactatt tcatttgagt cagctgataa cacaggtctt
    17821 actgatgata tgagtatttt tttaaaacca tttaacgcga aagacttaga agagttatca
    17881 tttgttgaag gagtagaaag aatagctcct tcaagagatg ggtttaacct tgattcagtt
    17941 tactcttcac aagcatcttt tgataaaaaa actacgtatg ttgatgttgg accagtaaaa
    18001 aaagattcta aaataaattt aatttgtggt agagattttt ctttagatga tgaaaaaaga
    18061 aaagtaattt tacttacatt acaaagtaca agtgaaattt ttgaaaatcc agaagatgcc
    18121 ttaggtcatg gtatcaacat aaatggaact atatttgaga taataggtgt attggatgat
    18181 tctcagcaaa accaagctgg aggattcttt ggaggttacc aagatatgca atttactact
    18241 tcccttatac ctaaaaaagc ttttgacact ttaatgagtc aaaattctta ttcaaatgaa
    18301 atttaccaat tagacttagt ttcctctaaa ggctataatg taaatgaagt tgcaaacaat
    18361 gttatatcta agttatatga aatgcaccct ggtatcaatg gttcttatac tacaccagac
    18421 ccaactgaac agacagctta tcttgaaagc attaattcca atgtaaacaa atatgtatct
    18481 ataataactg tagttgcaat gtttgttggt ggtattggtg taatgaatat aatgtatgta
    18541 tctgttatgg aaagacaaag agaaattggt ataagacgtg ctattggtgc aaagcctagg
    18601 tctatattat ttcaattttt agttgaagct gtttttataa cagtatgtgg tggaatttta
    18661 ggaactatag ttggatttgc tgctacaaac tatgtttcta aatatatagg ctttgaagca
    18721 atacctagct taaatagctt gttttatgca attatagcta ctatattgac tggagttgtc
    18781 tttggtctta taccagcctt taaagcctct aaactagacc ctataaaggc aatttataag
    18841 tagaaattgt ataaagattt tcgattttta aattagttta tataaaacat acttaaatat
    18901 ataacgcact attatatatg atagattaat aatttaatct ttccaaatat aatagtgcgt
    18961 tctttcataa aagggattct cttccttcta tatctaaatt accaatatgt cataccctaa
    19021 ctgttcaagc taatatttcc aatttggatt aatcaatctc cttttcctaa tatacaatct
    19081 tttgctctca acaattgtat cacccacctt aaaaaaataa tcttaaatta ggcgttatat
    19141 acaatcttcc cattacatat tgtataatta atcttgccat acaaagattt acctttaaac
    19201 ggtgaattat cagctttaga aacaaaactt tcaactgtcc attcttcttc agggttgaat
    19261 ataacaatgt ccgctacagc cccttcttct ataaacccag tatttaaatt gtacaatttt
    19321 gcagggtttg tagacatttt ttctattaac tgcatcatac taagatgatt tttatgcact
    19381 aaatttgtta ttccaagagc taaggaagtc tcaagcccaa taattccact tggagctttt
    19441 ataaattctc tatctttttc ttctctacta tgaggcgcat ggtctgttgc tattatttca
    19501 atagtaccat catttaatcc ctctataatt ttatctctat cccatttact tcttaggggt
    19561 ggattcattt ttgcatttgt tccaaactcc aatacatctt cctctgtcaa tgtaaaatga
    19621 tgtggtgaag cttctgcaac tacatttgca cctaagtatt ttgcaaatct aaccatatca
    19681 acagatacac ctgaacttat atgttgtata tcaaccttag caccagtctt tagtgccaac
    19741 atacaatctc tagctaccat aacatcttct gctacacttg atgcaccttt taatcctagt
    19801 tctgaagcta tctttcctgc atttacacct ggatttccaa ctaaagaagg gtcttcttca
    19861 tgaaaactaa gtggtacatc taactctttt gccttaacca ttgcctccat aattagatta
    19921 ctatccatta aaggtaatcc atcatcagta aaccctggta caccagcttt tttaagagct
    19981 tccatatcaa ctaactcttt cccttcaaaa cctttagtta tagctgctgc ttgtaaaaca
    20041 tttatacatg cattttttga tttttcttta acataattaa aagtatcctc attatctaca
    20101 ataggattag tgtttgccat acatataaca gttgtaaatc cacctctagc agcagattta
    20161 gctccacttt ctatatcttc tttatatgta aacccagggt ctctaaagtg tacatgtaca
    20221 tccactaaac ctggagcaac tacattacca ctagcatcta ttattttttc atattcatca
    20281 ctttcatcaa atttacctat tttataaatt ttattttctt ttataattaa gtcaatattc
    20341 tcgtctcttt gacttttagg gtctattact ctaccatttt taattaaaat catattattt
    20401 ctcctttata tttttaattt atatagactc atataatatt atacattatt ttacatactt
    20461 aaatacaaaa ttacgtttga catcaatagc ctttttagga caaagttcat ggcaacagta
    20521 acatcttata catttgctta agtctataat agcaccctta gtccctattt ctatagtagc
    20581 tgcaggacaa gcttccttgc attttccaca ttttatacaa tcttgaaatc taactacagg
    20641 ttttggtgtt ataagctttg aaataggctc atttaaaaat tttggtagct ttacggaaaa
    20701 caatcttaaa tctttactta catttggaac cttgtaattt tttataatta aatcgtttat
    20761 atcttcacct agaatttcaa tatctgaaaa atcttctcgt atataacctc tcttaatagc
    20821 acctctaatt gtagcaactc tgttgggaat taaacttata atcttacacg caacaacgtc
    20881 tatagcatat ggattttgtg atgctaataa aactcctatt tctctagtag tgccagctga
    20941 gggtccatca ccttccatac ctactatacc atccataatt gtcaatgtcg gttttatata
    21001 atcacaaata tctaaaagta cattttcaca aaacaactct tctgtaggaa atctataatg
    21061 tatttctgct ttttttaatc cagctataca tccatataag ttttttgtac caccagtaaa
    21121 agttgccatt atatgagtct ttaatttaca aagatttatt atgtaatctg aatctactac
    21181 tggtttaata atatccatat attttagaac atgtgctttt tcactatgta ccttaacatc
    21241 tgaaacatca taatttaact taatgcccaa ttcatttgct acttcttcaa ttccagatgc
    21301 cttataaata ctcttaagag aactttgatt atatggacca ccagggctat ctgctattgt
    21361 aatatcacaa ttcaactcta aaagtttttc acatacaact tttacaacca taggatgtgt
    21421 agttgtagct tcttcaggct tttttttcat caataaattt ggttttataa gtactttact
    21481 ccctttttta acaaacttgt ctataccacc aatatcctct aaattttttt caatagataa
    21541 ccttacttct tcataattgt aagttttaca actcctaaca gatactgact tcatattatc
    21601 tccatccttc tatcacaaca gtttatttgt ataatttaaa aaaggagaaa ccaaagtttc
    21661 tccttttaat ctatgtttta attattattt tccaaaatat ctgtttaata atcctaagaa
    21721 tgctttacca tgtctagctt cgtctttaca catttcatgt actgtatcat gtatagcatc
    21781 taagcctaac tctttagctc tcttagctaa ttttaatttt ccatcagttg caccatattc
    21841 agcatcaact ctaactctta agttttcttt tgtatctgca actacaactt ctccaagaag
    21901 ttctgcaaat ttagcagcat gttcagcttc ttcaaaagct attctcttat aagcttctgc
    21961 tacttctgga taaccttctc tatcagcttg tctactcatt gctaaataca ttcctacttc
    22021 tgtacattca ccagtaaagt tcgctcttaa tccttcaatt atttctgcat ctacaccttg
    22081 agctactcct attctgtgtt catcagccca aaccatttcg cctttcattt cttcaaattt
    22141 atcagctcca actttacata ctggacattg tgctggtgca gcatctcctt catgtatata
    22201 tccacatact gtacaaacaa attttttcat aattaacttc ctcccaattt tatataatta
    22261 aatttttatt tttaagttta ataacttgct acaagaatat atatacccca gactttgtag
    22321 cttaaacact aaaaataaaa tttatttcct aattaatttt atcactttga aacaacagta
    22381 tcaaccaagt tttgatgtca aatttctaca taatacataa tttatttaat taattaggaa
    22441 ataattgtaa tattaaatac tttccaatga agactcttct gaatctttaa gcttcacttt
    22501 tttgctcttt aaaaaagata atttggtttc tgcagttatg attgcaacaa atatcaagat
    22561 actaccaata accaattttg ctgtaataag ttcctttaga attattatag aaaatattgt
    22621 accaaataca gcttctgtgg ataatattat agcagttcta gttccatcaa ctcttttttg
    22681 acatatcgtt tgaaataaaa atccaattgt agtactaaat acagctaggt acatagttcc
    22741 catatagcca gataactcag cctccatctt taattgacct gcaaatgctt gaactatcac
    22801 agacattaaa aatgctactg taaattgaat ggctgtcaat gccattggat tattatcttt
    22861 tgcaaactca ctcgtaaaga atatatgaaa tgcaaatcca aatgaacata taagtgttag
    22921 aaaatctcca aaattaatag agaaatcagc ttctaaagaa agtatcccaa ttcctataag
    22981 agctaccaag ctacttatta tccctatctt atcaagtttt cttctataaa gtataaatcc
    23041 tatgaatgga actattacaa cattagctgc tgttataaac gcattcttag atggtgtagt
    23101 gtacataagt ccaatagttt gtacggtgaa ggctagaaat aaaaatattc ctaaaatccc
    23161 tcctgctttt aataattttt tacccatatt ggcacgaatc tgtttaaaaa acagtaaatt
    23221 cataattata gctgccaaga aaaatcttaa agttataatt tgtagaggtg taagcccacc
    23281 gtctaatgca aattgggttg ctacaaagcc acttccccat ataattgcta taaatattag
    23341 tcctatttct ccaaagtatt ttttcaataa agccccctcc tttttatttt tttgcaaaat
    23401 tataaacccc ctaaacagca agagagtttt tctataagca ttactataaa ttaatatagt
    23461 aaattttttc agtattttaa atctaaattt acatatattt tgagctcaaa tttatatcca
    23521 ccatttaaca gtaatatatt aatcattttt tgtaaacacc tttttgataa atttttttgt
    23581 tttttatatt tttttaccta tactaa
//
LOCUS       DAFNNB010000045        23572 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0045, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000045 DAFNNB010000000
VERSION     DAFNNB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23572
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0045"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(287..562)
                     /locus_tag="L3M60_001563"
                     /pseudo
     CDS             complement(287..562)
                     /locus_tag="L3M60_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898145.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            complement(949..1728)
                     /locus_tag="L3M60_001564"
     CDS             complement(949..1728)
                     /locus_tag="L3M60_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HBN6226241.1"
                     /translation="MHKMKKLVVRADDIGYSEGVTLGIISSYKNGIVTSAGIMVNMPF
                     SRQAIEMVKNDDLCFGLHVNLVIGEPCCKEHIKIKNLLDEGGKFVSSKIRRTQLLMGK
                     EVFNYEEVYEEVCAQIETYKLYMSKLPEYIDIHGIEEEVSISAMCDAAKEYGIPSCPY
                     YLDSCISMPSKYPQNDFYKLNKSFVKLFEEKYINLDKDITLMVTHPGFIDYSLIKSSS
                     LVQERVYEHAMLCDKEVKMWLRRNNIELVDFKDIFKANNYI"
     gene            complement(1721..3148)
                     /locus_tag="L3M60_001565"
     CDS             complement(1721..3148)
                     /locus_tag="L3M60_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBN6226242.1"
                     /translation="MMQQLSLLGKTMLVPIAVMPAAGILGLLFGPNMLNVPAISNIST
                     IVFSNVDYLFLLGAASAYSKSKDKTSVMVAAVVAYMIFKSNLELLNDTLNAGVFGGII
                     VGACIAWTYNKTYKVKVPAFLSFFAGEKCVITLAPVVALILSYIFSLLWIYPQAAMNQ
                     FGFWLGGAGAIGIFLFLFCNRALIPTGLHQVLNAYILFEMGEYTTPTGEVIRGEIPRF
                     MAGDPNAGFFWTGFYIIMMFSIPAISYAIYKTAQDKRKQEVKGAMTAGALTSFMATVT
                     EPIEFSFLFASPKLYIIHSFYSGLGGVVLYLLGARLGSFNGASIIDYVLSFSYGDKAW
                     LVIPIGIIFFVLYYVTFKYIIIKDNVQTPGREVEIEIGDKVSEKEKNLKLSHGNFSYM
                     AKQIIKNCGGYENIVTLNNCMTRLRLEVKDATILNDDNIKKTGAKGVIKLSNTSVQII
                     IGTDVVKVKDEMEIQLDELRKQADA"
     gene            3539..4261
                     /locus_tag="L3M60_001566"
     CDS             3539..4261
                     /locus_tag="L3M60_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226243.1"
                     /translation="MAKNKFSEIYEVLKEEILDGKYTSNMMLPTELQLIERFSCSRNT
                     VRRAISQLNAEGYVQSIKGKGVVVLENSCSNDFFLNMHNFKGVESIVEDKKVNTATSV
                     LHFSKILIDNKLSKKTGFKIGSEVYYLHRLRYIDNVPKILDINYFLCSIVKDLDVSIA
                     QGSIYKYIEECLGTKIVSSRKIFKIEKATELELKTLPLNDYNCVGVIKNSVYTDDGKL
                     FEYTESKHTPETFVFMDVTQRY"
     gene            4458..6125
                     /locus_tag="L3M60_001567"
     CDS             4458..6125
                     /locus_tag="L3M60_001567"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBN6226244.1"
                     /translation="MKNWWKKATVYQIYPKSFKDSNNDGIGDINGIIEKLDYLSSLGV
                     DLLWLTPMYVSPQRDNGYDIEDYYNIDPKYGTMSDFEELLKEAHKRDLKIMMDMVLNH
                     TSTEHKWFKESKKSKDNPYRDYYFWKDAKPDGSVPNNWISRFSGTAWKYDKTTNQYYL
                     HLFEETQADLNWENEKVREECYKILEFWADKGIDGFRLDVVNLLSKTPGLPDDPITGP
                     KGDGRTHYADGPRIHEYLHNMNQKVFKPKNIVTVGEMSSTTPEECINYTRENREELSM
                     VFSFHHMKTDYKGGVKWTNEMFSLDKLKKAQSDFQYKMYEGKGWNALFYSNHDQPRAL
                     SRFGDDRFFRQESAKMLAITLFGLQGTTYIYQGEEIGMTNAYFTKIDEYDDAESKNAY
                     YHMIKSGIDEKEALIILQQKSRDNARTPMQWDNTIYKGFSNHKPWLKVNNDNISVENE
                     LNDSHSVFYTYQRLIKYRKQYDIFTEGTYRLLDENHKNLFVYEREYKNQNILVISNFT
                     HDNVVYKLPETYLNKLNYTILETNYTRDTIENEMEIKPYESIMIYCK"
     gene            complement(6220..7539)
                     /locus_tag="L3M60_001568"
     CDS             complement(6220..7539)
                     /locus_tag="L3M60_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN6226245.1"
                     /translation="MEKGIKLGFWSIVLLGINAIVGSGIFGLPGDAYTDIGPASILVL
                     VFCMLLAVSIALCFAEAGSWFDTDGGPYLYAKEAFGNFIGFEVGFMKWIVSMIAWATM
                     ANFFAVTLSSVWPQAAEPLTKNIIIGILVVGLGIINFMGMKQSKQLNNIMTIGKLLPI
                     VLFIAVGLFFIKGSNFTPFVIIQKGQSSSSAFVAVSITLFYAFTGFESLAVAAKDMEN
                     PKKNVPKALVMVMFVVSVIYMLILGISIGVLGNGLVGSTTPVADAAIKMLGPIGGYII
                     TIGTIVSVGGINIASSIFTPRSAAALVEQGLMPKSIRKTNKNGAPYIAIIVSVIGTLL
                     IAWSGSFTTLSQISVVSRFIQYIPTCLAVLILRKKYAGKDVNFRIPGGAIIPIFAVII
                     SILLLIKAGIDEPMKIVWGLGGMIIVVPIYFYMTKVYPKKYNNVEVK"
     gene            complement(7577..8329)
                     /locus_tag="L3M60_001569"
     CDS             complement(7577..8329)
                     /locus_tag="L3M60_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HBN6226246.1"
                     /translation="MKKLVKKKYPIIGISGNLLIDEGGMFPGYERAYVNNDYIQSVVM
                     CEAIPYIVPIVYDDEIIKEQVSNIDALILSGGQDINPLIWKEEPHNKLGAISPKRDVF
                     DMKLLKYALDMKKPVLGICRGEQIINVAEGGSLYQDLSLIEGAYIKHNQQHLSNIPTH
                     TALIKEDTKLYEILGEKEILVNSFHHLVVNKVAPGYIVSATSKDGLIEAIEKEGSEFV
                     IGIQWHPEMMTKDYDNMKKIFMAIVKEASKEK"
     gene            complement(8573..10582)
                     /locus_tag="L3M60_001570"
     CDS             complement(8573..10582)
                     /locus_tag="L3M60_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6226247.1"
                     /translation="MKTIAVVTDGLSKLECFLNKNLEMIFKDKVMIKNYYLNKITEKE
                     LIKDDIILVMIDDRVLKIKKYVEDVSKIITINRSIKQKDIYKLFSLPEGIDVLVVNDN
                     KHTVLETISNLYNIGINHLNFIPYNPNEEYRNIKIAITPGEASLVPKYIEEVIDLGHR
                     YIDSSTFIQIIAKLKLDDKEITERLIKYTDEIISLYSGINTKYKELTIKADELNSIIN
                     LSNLGIAMISDKGNVIICNNSLSNILDIQGNIIGKNIDELENENIKKIFRLSKAHDEV
                     IKFNNKYLNVNKYNIESFGRVTGYYFCIQEITYIRKLEQNLSKKLREKGQVARYTFDD
                     IKTVSLKMERTKELGMKIAKSDYTVLITGESGTGKELMAQSIHNASPRHSQPFIAINC
                     AAMPENLLESELFGYEEGAFTGALKGGKKGLFEQANNGTIFLDEIGDMPIYLQSKLLR
                     VLQENQVMRVGGENVIDIDVRVIAATNKNLLEMIKEDRFRADLYYRINVLPVNIPPLR
                     ERKEDIEVMLKHFMKRKRAISQDVQDIINAYEWPGNIRELKNTAMYINIMSSEDVILI
                     SDLPHNLLNITEDFSKEISILKDRTSLEKVKLVMGCLKNANNLNKNIGRNGIVNSINE
                     LGYSITEGEVRGILNILKELDLIICESGRRGSELNRRGKNILNSM"
     gene            complement(11018..12451)
                     /locus_tag="L3M60_001571"
     CDS             complement(11018..12451)
                     /locus_tag="L3M60_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBN6226248.1"
                     /translation="MSFKKDFLWGGATAANQCEGAWNLDGKGTSCSDMCTGGSQTVSK
                     RITRTIEKGTFYPSHEAIDFYHRYKEDIALFAEMGFKVFRFSIAWTRIFPTGMEKEPN
                     EAGLKFYENVIDECLKYNIEPLITISHYEVPFAITEKYNGWASREVIDLYMNYCEAIF
                     KRYKGKVKYWLTFNEINSATMPMGGYLSQGILNEGTTDFISQVDIPQLRFQGLHHQFV
                     ASAKAVKLAHEIDSNYQVGCMQIFATMYPYTCNPNDVVKTQEDNRVMNYFCGDVQVRG
                     EYPTYMNRYFRENNIEIKMEKGDLEILKEGCVDFYTFSYYMSTCVSSESKESNTSGNI
                     LGGVKNPYLESSEWGWQIDPKGLRYALNEIYDRYRIPMMVVENGLGAYDKKEPDGVIN
                     DDYRIEYLKAHIEQMKEAVEDGVDLFGYTPWGCIDLVSASTGEMAKRYGFIYVDKYDD
                     GSGDLSRIKKKSFNWYKQVIESNGERL"
     gene            complement(12467..13891)
                     /gene="ascB"
                     /locus_tag="L3M60_001572"
     CDS             complement(12467..13891)
                     /gene="ascB"
                     /locus_tag="L3M60_001572"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBN6226249.1"
                     /translation="MALRKDFLWGGATAANQCEGGYKEGGRGLANVDVIPQGKDRFPV
                     LTGKMKMFECDEEHYYPAHEAIDMYHNYKEDIKLFAEMGFKCYRMSIAWTRIFPTGIE
                     EVPNEEGLKFYEDLFDECLKYGIEPVVTICHFDVPMYLVKKYGAWRNRKLVDLYVKYC
                     EVIFTRFKDKIKYWLTFNEINMILHLPFIGAGLYIEEGENEEQIKYQAAHHQLVASAL
                     VTKLAHNINQEFKIGCMIAAGNTYANTCNPLDVWKSIEKDRENYFFIDVQSRGEYPAY
                     ALKELERKGINIVMEEDDKKILKENTVDFISFSYYSSRLTSADPEVNILTEGNVFESL
                     KNPYLDASEWGWQIDPLGLRITINSLYDRYQKPLFIVENGLGAVDNPDETGNINDDYR
                     IDYLRAHIKAMIDAVELDGVDLWGYTPWGCIDLVSASTGEMKKRYGFIYVDKDNDGNG
                     TLKRSKKKSFDWYKKVIESNGKEL"
     gene            complement(13978..15894)
                     /locus_tag="L3M60_001573"
     CDS             complement(13978..15894)
                     /locus_tag="L3M60_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6226250.1"
                     /translation="MRKYEQLSKEIIENVGGKDNISSLSHCITRLRFKLKDESIANDD
                     VLKKMDGIVTVMKSTGQYQVVIGNHVPDVYEVVCDLAGLGGDTIDNSGSDSPKGLLNK
                     FIDIISGVFQPILGVLCAAGMIKGFNAVFTALGLYGETDGAYIMLNAIGDAMFQYMPI
                     ILGYTAAKKFKLKPFIGMLIGAVLCYSGIQLDTLASTGQPLYNLFTGTIFESPVYITF
                     LGLPVIAMNYTSTVVPVILIVFLASKLEKMFAKIIPDVVKNFFVPMAVLLVSLPLGFM
                     VIGPIATYASNIVGNGFLALFNFSPILCGALTGLLWQVLVIFGLHWGLVPIAMSNLMT
                     MGFDTILVGAFVPSFAQTAVVAAMYFKLKDKKMKELCIPAIISGICGVTEPAIYGITL
                     PRKKPFIYSCVGGAVAGAVMGVMNVKSYVMGGLGIFGIPNYINPTTGDMTGVYATTIS
                     IIVAIVVGFVLTFFLWKDDTIVEISKDNNSKGAVEKNKREVIYSPIKGEVKALEHMQD
                     AAFSTGVLGKGVAITPKEGKVVAPFDGTVMTLFPTKHAIGIISDNGCELLIHIGLNTI
                     QLEGKYFKSFVNQGDKIKKGQTLLTFDIESISKEGYCLETPVVVTNYSDYVDIVENTQ
                     KHISNSQELITVLA"
     gene            complement(15968..16828)
                     /locus_tag="L3M60_001574"
     CDS             complement(15968..16828)
                     /locus_tag="L3M60_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6226251.1"
                     /translation="MIIEKILNNNVIITTDENHKEIVVMGRGLAYKKRTGDHISKDKI
                     DKIFKLSDPNICDKFKELIADIPMKYMELSDEIILYAKEKLGKRLNDSIYISLTDHMY
                     TAIERAREGVNVKNVLLWDIKRFYKNEFNIGLKALDYIEKKFEIRLPEDEAGFIALHI
                     VNAQMDQSIKIIYDITQIIQEIANVVKYHYRIVFDEDSVYYHRFITHLKYFAERVVSN
                     NLHENNEDDLLKVIKVKYKNAYKCIEKLDEFIHKKYNYNLTDEEKLYLTIHVERVVSK
                     SIKSDINPNN"
     gene            complement(17181..18368)
                     /locus_tag="L3M60_001575"
     CDS             complement(17181..18368)
                     /locus_tag="L3M60_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBN6226252.1"
                     /translation="MIESIKKRAYEIENELGKEIEEVCNFIFNNPELGEEEYISSKYL
                     VEKMEEYGFDTIYPYCNMETAFRAELGNNDGPTIAFLAEYDALPGYGENKEPAHACGH
                     NWIAASTLGACIVLSKLKENFKGKIVLIGTPAEETTGGKCDLVKAGAFDDVDVSYQMH
                     IEAFNNINCKALAIDSLEFSFEGVSAHAASHPHMGVNALDAVQLTFAGINALRQHVKS
                     DVRIHGIVSNGGEAPNIVPEKAACKFFVRAAERSYLDEVTKKVINCAKGAELMTGAKL
                     SYRYFENSFDNIINNKVLQKITKNNLIEAGITDILEGKDGPVGSTDIGNVSQVCPTMY
                     TEIALDINPMVYVHEKEFLSYANSEEAYDKLHKAVKAMVGCALEIYLEDGLLDEIKKN
                     HLN"
     gene            complement(18382..19773)
                     /locus_tag="L3M60_001576"
     CDS             complement(18382..19773)
                     /locus_tag="L3M60_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HBN6226253.1"
                     /translation="MSSLKKKKFTMPQTLTIIFLLIVLMAILTWIVPSGNFERVDIDG
                     RSVVVAGTYEKAPSNPQGITDVFTAPINGFIDAAEVVGFVLIVGGAFGIVNKTGAIEA
                     VIAHTVNKMKRFQFLIIPISMILFGLGGTTFGMSEETLPFYMIFIPLMTSMGYDSLTA
                     VATVFIGATAGFGAATTNPFSVGIAQALSQITPGSGIEFRVVMFIIYMAISIGFVMMY
                     ANKVKKDPKKSLVHDISLNQESMVNSDSNIKEFTKREAMVIAIFTIGMAIMIYGVLKL
                     GWYITEIAMIFTAIGIISGIASGLKQDDIVNSFISGAGDLMTAGLCIAFARGIVIIAE
                     NGFIIDTILNSAANLLNGLPKTIFINLTFLIEGLIAFLIPSASGLASLTIPVLAPLGD
                     LVDVSRQMIVTAYQFGIGVTNLITPTSGVLMGALAVANIPWSKWVRFIIPLMVALTVL
                     VMVFLTIGLYLGF"
     gene            complement(20369..22069)
                     /locus_tag="L3M60_001577"
     CDS             complement(20369..22069)
                     /locus_tag="L3M60_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBN6226254.1"
                     /translation="MHYIAWHFNKNGGNNTMKDKLQVLSGAMMIPVILLVVAGVFIGL
                     GSAFANMENVYALGLSGIIKEGSFINVFFKVINDLGFMVMRYLPIFFVTGIAFGLSKK
                     EKGWGALGGIVLFIGMHTVISTLLAANGINSDTVTAEAFMASGLGEAEAYSRSSLYGS
                     FLGIFSYDCSIFGAIIAGFVASAVHNKFVDIELPSALSFFSGPRFSMIMMFIASIVLG
                     TAMYFIWPPVGLALSKLGNWIGSSGLLGTFTFGAMDKALLPFGIHHLIAFPIEYTRVG
                     GTMEVGGAIYEGVNNIRLAQMGDPDTLSYITRNFTTGRLLIHFAILPAAALAMYKLAD
                     NVNKKKAISILIPAIVTAMLVGVTEPIEYTFLFVAPLLYFLAYVPLAGLTYVFTEMFN
                     VSIMGESFRNMFPNLLQPQKVDALPLLFLIPIFFIVTYIIFTWAIKKFDIKTPGRSSE
                     EVKLYSKKEYREKQSVDAGKTVYNNAVGDEVDDKDTELVHSIIEGLGGSSNIKNVTNC
                     ATRLRVELNSIDGFYDDGFWVNELGASGVVKKKNSVQVIFGPRVITIASKLKAVLGVD
                     "
     gene            complement(22041..23444)
                     /locus_tag="L3M60_001578"
     CDS             complement(22041..23444)
                     /locus_tag="L3M60_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HBN6226255.1"
                     /translation="MIVNKELIIDYLKSNQENIIKDIRSLVEIPSIRDESTTDINQPF
                     GIEIRNAFDKLIKIAKDKDFVVKDFDGYAIHIEYGEGEEVVGVLNHIDVVPIYNKELW
                     KSKPFKVCQKDNYLYGRGVNDNKGPLIGILYALLFLRELNEKPKRKIRLIVGGAEETT
                     WECMEHYFSVNEQPKFAFSPDGNFPIVNGEKGILYFNLRKKIDKDKFRNHNLVDIKSN
                     KEDGFVCDKIEAVFKTNNKKDLVESLAYYTEIEEVEEGKVLVRYTGERALSRNPHRSY
                     NCAFSLAKDLERIKGLNDKGVIIKDILNSYFTDDNHGKKIGIYKEDVDMGVSTICIMS
                     IFLDKNELNMKIDFRYPKGISLEFITNRINEIGKKENLIIDIYKNLKLLYVEPDSELI
                     NKLSSAYKQGFEKEAELFTKGAASYARVLENGVAFGPTIEGDNPNSHQANENISIDTL
                     YKAIEVYIYALYSLAFQ"
BASE COUNT         7652 a         4285 c         2607 g         9028 t
ORIGIN      
        1 gtctatatat gtaaatttat aatatcataa ttttttactt aaagtttatt atgttatttt
       61 atagcatttt aagataaaaa agaattgtac cgctactaaa atccggctga aatacaataa
      121 atattcctca tcctgatatg tttataatta aaggtataaa tataaacttt gctatgaatc
      181 acataattaa taaatcattt tatttacaat tataaccccc cttataaaac tatattaata
      241 atatatacag tatttataaa ggaggttatt tatatttaaa aactatttac ttttctctta
      301 attcttctcc attagttctt attacatttt gataccaata aaaactatct tttttataac
      361 gttctttgtt tccatttcca ttattatcta tatccacata tataaatcca tatctttttt
      421 ccatttcctg agatgaacaa cttactatat ctattggtga ccacatacaa taagctaata
      481 tttcagttcc atcagctata gcttcaccta tttgctctat atgttttgat aaataatcta
      541 tcctgtaact atcatgaact tttcagatac tcataatctc ggttttgata ataaagtata
      601 catagttatt actactttac caattagtga tagatatcct ataccctttg tttatgtaaa
      661 tccttaccta aacggaaaga attatgacaa tatccaaatt aaatttcttc atattataag
      721 gtagaaaaaa tatacttatt gtacaaaacc atttagaaat gtactttagt gaatctttat
      781 ttatgaaaaa tatctatctc tttttattat acataaaaaa tgtgtacttc ctatattgat
      841 agggatgatt caaattttta ttttgataca tctctatcaa tattctttta gaaaatacac
      901 atttttttaa atacttattt ttaattaaaa ctttaagtaa tctattcttt atatataatt
      961 attagcctta aaaatatctt taaaatcaac caattcaata ttatttcttc ttaaccacat
     1021 tttaacttcc ttatcacata gcattgcatg ttcatatact ctttcttgaa caagagaaga
     1081 tgattttata agactataat ctataaatcc tggatgagta accatcaatg ttatatcttt
     1141 atctaaattt atatactttt cttcaaataa tttaacaaaa gatttattta acttataaaa
     1201 atcattctga ggatatttac ttggcatact aatacatgaa tctaaataat agggacaact
     1261 tggtatacca tattctttag cagcatcaca cattgctgat attgatacct cttcttctat
     1321 accatgtata tcaatatact caggcaactt actcatatac aacttgtaag tttcaatttg
     1381 agcacaaact tcttcatata cttcttcata attaaaaacc tctttaccca tcagtaactg
     1441 cgttcttctt attttgctag aaacaaattt tccaccttca tctaacaaat tctttatctt
     1501 aatatgttct ttgcaacaag gttctcctat tactaagtta acatgtaaac caaaacataa
     1561 atcatcattt tttaccattt ctatggcttg tcttgaaaaa ggcatattca ccatgattcc
     1621 ggcacttgta acaataccat tcttataaga agatattata ccgagtgtaa caccttctga
     1681 atatcctata tcatctgccc taactactaa tttcttcatt ttatgcatct gcctgctttc
     1741 tcaactcatc aagttgtatt tccatctcat cctttacttt aacaacatcc gttcctatga
     1801 ttatttgaac tgatgtatta ctcaatttaa ttaccccttt agctcctgtt tttttaatat
     1861 tatcatcatt caaaatagtg gcatctttca cttctaaacg caaccttgtc atacaattat
     1921 taagagtcac tatattttca taaccaccac aatttttaat tatctgctta gccatatatg
     1981 aaaaattacc atgagaaagt tttaaattct tttctttttc actaacttta tcacctatct
     2041 ctatctcaac ttcacgtcct ggtgtttgta cattatcttt tattattata tatttaaatg
     2101 ttacataata taatacaaag aatataattc ctataggtat aactaaccat gctttatctc
     2161 cataactaaa acttaaaaca taatcaatta tacttgctcc attaaatgaa cccaagcgag
     2221 cacccagtaa atatagtaca actccaccta gaccagaata aaaagaatga attatatata
     2281 actttggaga tgcaaataaa aacgaaaatt ctataggttc agttactgtt gccataaaag
     2341 aagtcaatgc cccagctgtc attgctcctt ttacttcttg tttcctttta tcttgtgctg
     2401 ttttatatat agcataagaa atagcaggta ttgagaacat cataattatg taaaatcctg
     2461 tccagaagaa tcctgcattt gggtctcctg ccataaatct aggaatttct cctcttataa
     2521 cttctcctgt tggtgttgta tattcaccca tttcaaataa tatgtaagca tttaaaactt
     2581 gatgtaaccc agttggtatt agtgcacggt tacagaataa aaataagaat attcctattg
     2641 caccagctcc tcccaaccaa aaaccaaatt gattcattgc agcttgtgga tatatccata
     2701 ataaactaaa tatatatgat aaaattaaag caactactgg tgctagtgta attacacatt
     2761 tttctcctgc aaagaagctt aaaaatgctg gaactttaac tttatatgtt ttattgtaag
     2821 tccatgctat acaggctcca acaattatcc caccaaatac acctgcattt aatgtatcat
     2881 tcaataactc taaatttgac ttaaatatca tatatgcaac tactgctgca accattacac
     2941 ttgtcttatc tttagacttt gagtaggcac tagctgctcc taataaaaat aagtagtcca
     3001 catttgaaaa tactatagta gatatattag atatagctgg tacattaagc atatttggtc
     3061 caaataataa tcctaatata cctgctgctg gcattacagc tattggcact agcatagttt
     3121 ttcctaaaag tgacagttgt tgcatcattt tgtttaagaa tctcttcata tccaatcccc
     3181 tttctatttc ggtttaattt tttgtgaaaa cgatttgttt acattttata ttttaaatat
     3241 tatatttgtt taaacaaatt gtcaatattt tttatttatt ttatcttcat catttagttt
     3301 cacatctaaa ctcttttgag tatattgcta tatctagcct atttttttag ttcatttttt
     3361 ttatttttaa aatatgttta accatttatt tgaagtaagc tacaaaatag ttcagtttta
     3421 tcttttttat agcttatata attctatttt cactatacat gtctaaacac atttgatata
     3481 atttgttcat atcaattcaa taatattact aaagaccttt aaggaggggt aggggtatat
     3541 ggctaaaaat aaattttcag agatatatga agtattaaaa gaagaaatac ttgatggtaa
     3601 atatacaagt aatatgatgt tgcctactga gcttcaactt attgaaaggt tttcatgtag
     3661 cagaaacact gtacgtaggg caatttctca attaaacgca gaaggatatg ttcaaagtat
     3721 aaaaggcaag ggtgttgtag tgttagaaaa tagttgttct aatgattttt ttctaaatat
     3781 gcataatttc aaaggtgtag aatcgattgt agaagataaa aaggttaata ctgctactag
     3841 tgttctccac ttcagcaaaa tcttaattga taataagtta tctaaaaaga caggttttaa
     3901 gattggtagt gaagtatatt atcttcacag actaagatat attgataatg tacctaaaat
     3961 actagacatt aactattttc tatgcagtat agttaaagac cttgatgttt ctatagcaca
     4021 aggctctatt tataaatata tagaagagtg tctaggtaca aaaattgttt catccagaaa
     4081 gatatttaaa attgaaaaag ctacagagct tgagttaaaa acgctacctc taaatgatta
     4141 taattgtgtt ggtgttatta aaaatagtgt atatactgat gatggaaagc tttttgaata
     4201 tactgaatca aagcatacac ctgaaacatt tgtattcatg gatgttactc aaagatatta
     4261 aaaaaattat gtttaattaa acataatttt tttacgttta gacactttat actatactaa
     4321 tctcactatt ctataaataa ttatatgttt atacaaataa tcatttattt tatattttac
     4381 ctttagtttc agttttattc ttgacaattt gtttaaacat attataatta acttataaat
     4441 tacaggaggt atcaagtatg aaaaattggt ggaaaaaagc aaccgtctat caaatatatc
     4501 caaaaagttt caaagatagc aacaatgatg gtataggtga tataaatggt attatagaaa
     4561 aattagatta tctttcttca ttaggtgttg atttattatg gttaactcct atgtacgttt
     4621 ctcctcaaag agataacgga tatgatatag aagattatta taatatagac ccaaaatatg
     4681 ggacaatgag tgactttgaa gagctcttaa aggaagctca taaaagagac ttaaaaataa
     4741 tgatggatat ggttctaaac catacatcta ctgaacacaa atggtttaaa gaatctaaaa
     4801 aaagtaaaga caacccatat cgtgattatt atttctggaa ggatgctaaa cctgatggtt
     4861 ctgtacctaa taactggata tccaggttta gtggaactgc ttggaaatat gataaaacta
     4921 ctaaccaata ttatttacat ttatttgagg aaactcaagc tgatttaaat tgggaaaatg
     4981 aaaaagttcg tgaagaatgt tacaaaatac tagaattttg ggccgataaa ggtattgatg
     5041 gttttcgttt ggatgtggtc aacctactat ccaaaacacc tggattacct gatgacccta
     5101 taacagggcc aaagggagat ggaagaactc actacgctga tggtcctcgt atacacgaat
     5161 atttacataa tatgaatcaa aaagttttca aacctaaaaa tatagttact gtaggtgaaa
     5221 tgtcatccac tacacctgag gaatgtatca actatactag agaaaatcga gaagaactat
     5281 ctatggtttt tagctttcat catatgaaaa cagattataa aggaggcgta aaatggacta
     5341 atgaaatgtt ctctttggat aaattaaaaa aagcacaatc tgactttcaa tataaaatgt
     5401 atgaaggaaa aggatggaat gctctatttt actctaatca tgaccaacct agagctttgt
     5461 ctcgattcgg agatgataga ttttttcgtc aagaaagtgc taaaatgtta gccataacac
     5521 tatttggttt acaaggtact acctatatat atcaaggtga agaaataggt atgacaaatg
     5581 cctactttac taaaatagat gaatatgatg atgccgagag taaaaatgca tactatcata
     5641 tgattaaatc tggcattgat gaaaaagaag cattgattat acttcaacag aaatctagag
     5701 acaatgctcg tactcctatg caatgggata ataccattta taagggattt tctaatcata
     5761 aaccgtggct aaaagttaat aatgacaaca ttagtgtaga aaatgagctt aatgattctc
     5821 attcagtatt ttatacttat caaagattaa taaaatatcg taaacaatat gatattttta
     5881 cagaaggtac ttatagattg ttagatgaaa atcataaaaa tctatttgtt tatgaaagag
     5941 agtataaaaa ccaaaatatt cttgttataa gtaacttcac tcatgataat gtagtgtata
     6001 aattacctga aacctatttg aataaattaa actacacaat acttgaaacc aattacacta
     6061 gagatacaat tgaaaatgaa atggaaataa aaccttacga aagtataatg atatattgta
     6121 aataaagtta tttatgtaaa aatcggtata acatcagagt gttataccga tttactaaat
     6181 tcaaaataat atttatagaa tctctataaa ttacaataat tatttgactt ctacattatt
     6241 atatttcttt ggatatactt tagtcatata gaaatatata ggaactacaa ttatcatccc
     6301 acctaatccc caaactatct tcataggctc atctatacct gcttttatca gtaataatat
     6361 acttataata actgcaaata ttggtataat tgctcctcca ggtattctaa aatttacatc
     6421 tttaccagca tacttctttc tcaatattag tacagctaag caggtaggaa tatattgaat
     6481 aaatctagat acaacactta tttgagataa tgttgtaaat gagcccgacc atgctattag
     6541 tagagttcct atgacagata ctataattgc tatatatgga gctccatttt tattagtttt
     6601 tcttatagac ttaggcatta atccttgttc aaccaaagca gcagcagacc ttggtgtaaa
     6661 tatggatgat gcaatattta tacctccaac agatactatg gttcctattg taattatata
     6721 cccacctata gggcctaaca tcttaatggc agcatctgca acaggagtag ttgaacctac
     6781 taagccatta cctaatactc ctatacttat tcctagtata agcatatata ttactgatac
     6841 tacaaacatt accattacca gtgctttagg tacatttttc ttagggttct ccatatcttt
     6901 tgctgcaaca gctaaagatt caaaccctgt aaaagcataa aataaagtta tagatactgc
     6961 tacaaaagct gatgatgaac tttgaccttt ttgtattatt acaaatggtg taaaattaga
     7021 accctttata aagaataatc ctacagctat aaataaaact attggtaata attttcctat
     7081 agtcattata ttatttaatt gcttagattg tttcatcccc ataaagttta taatcccaag
     7141 ccctacaact aatataccaa ttattatatt tttagtaagt ggttctgctg cttgtggcca
     7201 tacagatgat agtgttacag caaagaaatt tgccatagtc gcccaagcaa tcatactaac
     7261 tatccacttc ataaatccaa cttcaaatcc aataaaattt ccaaatgcct cttttgcata
     7321 aagatatgga cccccatcag tatcaaacca actacctgct tcagcaaaac ataaagcaat
     7381 agaaacagct aaaagcatac aaaaaactaa tactaatata cttgctggac ctatatctgt
     7441 ataagcatca ccaggtaatc caaatatacc tgaccctact attgcattga ttcctaatag
     7501 tacaatactc caaaaaccta acttaatgcc tttttccatt gttattcccc ccaaagccaa
     7561 cttaatatag ttataattat ttttcttttg atgcttcttt aactattgcc ataaatattt
     7621 ttttcatatt atcataatct ttagtcatca tttctggatg ccattgtatt cctattacaa
     7681 actcacttcc ttccttttct atagcttcaa ttaaaccgtc tttagatgta gctgaaacaa
     7741 tatatcctgg tgctactttg tttacaacta aatgatgaaa actattaact aaaatttctt
     7801 tttctcctaa tatttcatat aattttgtat cctcttttat cagcgctgta tgtgtaggta
     7861 tgttagataa atgttgttga ttgtgtttaa tataagcgcc ttcaattaga gataaatctt
     7921 gatacaaact acctccctca gctacattta taatttgttc tcctctacat atacctaaaa
     7981 ctggtttttt catatctaaa gcatatttta ataatttcat atcaaaaaca tctctttttg
     8041 gtgatattgc acctaactta ttatgaggtt cttctttcca tatgagtgga tttatatctt
     8101 gtcccccaga tagtataagc gcatctatat tagatacttg ttcctttata atctcatcat
     8161 catatacaat tggtacaata tatggtatag cttcgcacat aactactgat tgtatatagt
     8221 cattatttac ataagctctt tcatagccag gaaacattcc tccttcatca attaataaat
     8281 tacccgatat tcctattatt ggatatttct ttttaaccaa tttcttcacc ccttcactta
     8341 ttcgtaaaca ataaaaaatg ctacttctct taaagagcaa gtagcatacc aatattttat
     8401 ttatttgaat attataattt attttagaaa tacttttttt aaatactagt aatataaata
     8461 actttcttta cttatatttt attttaatcc tgtttagttt gactttttta aattgtttta
     8521 agtacaatta ataggtaaaa taggtattta tattttcctt tttattaacc tattacatgc
     8581 tgtttaatat atttttaccc cttctattta attcacttcc ccttctacca gattcacata
     8641 ttatcaagtc cagctctttt agtatattaa gtattcctct tacctcacct tctgttatac
     8701 tatagcctaa ttcatttatt gaattaacaa taccatttct acctatattt ttattaagat
     8761 tattggcatt ttttagacac cccatcacta actttacttt ttctaaagat gttctatcct
     8821 ttaatatact tatttcttta ctaaaatctt cagttatatt taataagtta tgaggtaaat
     8881 cacttattaa aataacatct tcagagctca taatgttaat atacatagca gtatttttta
     8941 attctcttat attacctggc cattcatatg cattaataat gtcttgaaca tcttgactaa
     9001 ttgctctctt ccttttcata aaatgcttca acataacttc tatatcttct tttcgctctc
     9061 ttaaaggcgg tatatttaca ggcaatacat ttatcctata gtataaatct gctctaaatc
     9121 tgtcttcttt tatcatttct aataaatttt tatttgtagc tgcaattact ctaacatcta
     9181 tatctattac attctctcct ccaactctca ttacctgatt ctcttgtaat actctaagta
     9241 atttgctttg taagtatata ggcatgtctc ctatttcgtc caaaaatata gttccattgt
     9301 tagcttgttc aaataatcct tttttacctc cttttaaagc tcctgtaaaa gcaccttctt
     9361 catatccaaa aagttcgctt tcaagtagat tttctggcat agctgcacag tttattgcta
     9421 taaatggttg actatgtctt ggtgatgcat tatgaataga ctgagccata agttcttttc
     9481 ctgttccact ttctccagtt ataagaaccg tataatcaga tttagctatt ttcataccta
     9541 attccttagt tctttccatc tttagcgaaa ctgtttttat atcgtcgaaa gtatatctag
     9601 caacttgtcc cttttctcta agcttctttg ataggttttg ctccaatttt cttatgtaag
     9661 ttatttcttg aatacaaaaa taatatccag taacccttcc aaaactctct atattatact
     9721 tatttacgtt taagtactta ttattaaatt ttataacttc atcatgtgcc ttactcaacc
     9781 taaaaatctt tttaatattc tcattctcta attcatctat atttttccct ataatattac
     9841 cttgaatatc tagtatatta cttagactat tattacaaat aattacgttt cccttatctg
     9901 atatcattgc aatacctaag tttgataaat ttattatgga atttaattca tctgctttta
     9961 tagtaagttc tttgtattta gtatttattc ctgagtataa actaattatt tcatctgtat
    10021 acttaattag cctctcagta atttccttat catccagttt gagttttgct attatttgaa
    10081 taaatgttga actatcaata tatctatgcc ctaaatcaat aacttcttct atgtattttg
    10141 gaactaaact tgcctctcct ggtgttatag ctatcttaat atttctatat tcttcattgg
    10201 gattatatgg tataaaattc aagtgattta ttcctatatt atataaatta gatattgttt
    10261 ccaatacagt atgtttatta tcgtttacta ctaatacatc tattccttct ggcaatgaga
    10321 ataacttata tatatccttt tgttttatac tcctatttat agttattatc tttgagacat
    10381 cttctacata tttttttatt tttaatactc tatcatctat cattaccaat attatgtcat
    10441 cttttatcag ctccttttct gttattttat ttaaataata attttttatc attactttat
    10501 ctttaaatat catttctaga tttttattta aaaaacattc taatttagac aatccatcag
    10561 ttactactgc tattgttttc attttattac tccttttaaa atatactcta ctcttttata
    10621 atatacattt gatttccact ttaatagcat aaaaaattgt acatttagta tactaacata
    10681 ttcatttatt agttttattt ccctaaacat catagttgca atatatatca tatttcttaa
    10741 gcttatacag caaaaaatgt gccaaattaa taattttctt acttttattt taaagttctt
    10801 caatcgtatt tacagactta gcatttataa attataagtc tttatattat taatctattt
    10861 ttataggtta attatacaaa tcatatacca cttttaccca ttaaattcat tataaaaaag
    10921 atggaataac gaattgttac tccacctttt ttgtaataaa aaattgtata tataaacacc
    10981 atttgacact cttctaagta caaacagtat ctacatgcta taacctttca ccattagatt
    11041 ctattacttg tttataccag ttaaaactct ttttcttaat cctagacaag tctccacttc
    11101 catcatcata cttgtccaca tatataaacc catatctctt ggccatctca ccagtagatg
    11161 cgcttactaa atctatacat ccccatggag tatatccaaa caaatcaaca ccatcttcta
    11221 cagcttcctt catttgctct atatgtgctt tcaaatactc tatacgatag tcatcattta
    11281 tcactccatc aggttctttt ttatcataag ctcctaaacc attttcaacc accatcatag
    11341 gtattctata tctatcataa atttcattta gtgcatatct aagcccttta gggtctattt
    11401 gccatcccca ttcactagac tcaagatatg ggttttttac tccacctaat atattgccac
    11461 ttgtattact ttctttagac tcactactta cacatgtaga catatagtaa ctaaatgtat
    11521 aaaaatctac acaaccttct tttaaaattt ctaaatctcc tttttccatt tttatctcaa
    11581 tattattttc tctaaaatat ctattcatat aagttggata ttcaccacgc acttgtacat
    11641 ctccacagaa gtaattcatt actctattat cttcttgagt cttaactaca tcatttggat
    11701 tacaagtata agggtacatt gttgcaaata tctgcataca tccaacttga taatttgaat
    11761 ctatttcatg agctaacttc acagccttag cacttgctac aaattgatga tgtaaccctt
    11821 gaaaacgaag ttgaggtata tctacttggc ttataaaatc tgtagttcct tcatttaata
    11881 tcccttgtga caagtaccct cccataggca ttgtagctga atttatttca ttaaatgtta
    11941 accaatactt tactttccct ttgtatcttt taaatattgc ctcacagtaa ttcatataca
    12001 agtctattac ttctctcgaa gcccatccat tgtatttctc tgttattgca aatgggactt
    12061 catagtgaga tattgttatt aatggttcta tattatattt taagcattca tctattacat
    12121 tttcataaaa tttaagccct gcttcatttg gttctttctc cattccagtt ggaaatattc
    12181 tagtccatgc tattgaaaat ctaaatacct taaatcccat ttctgcaaat aaagctatat
    12241 cttctttata tctatgataa aaatctattg cttcatgact tgggtagaac gttccttttt
    12301 ctatagttct tgttattctt ttacttactg tttgagaacc tccagtacac atatctgaac
    12361 aagatgtacc tttcccatct aaattccatg ctccttcaca ttgatttgct gctgttgctc
    12421 caccccataa aaaatctttt ttaaatgaca tatctatatc ctcctattat aattctttac
    12481 catttgattc tattactttt ttataccagt caaaactctt ctttttacta cgttttaaag
    12541 ttccgtttcc atcattatct ttatccacat atataaatcc atagcgcttc ttcatttctc
    12601 cagtactggc acttactaag tctatacatc cccatggtgt gtatccccac aaatcaacac
    12661 catctaactc aacagcatct atcatagctt ttatatgagc acgtaagtaa tcaattctat
    12721 agtcatcatt tatattccca gtttcatctg gattatctac tgcgcctaat ccattttcaa
    12781 ctataaataa tggtttttga taacggtcat acaaagaatt aatagttatt ctaagcccta
    12841 gtgggtctat ttgccatccc cactcacttg catctaaata tggattttta agactttcaa
    12901 aaacatttcc ttctgttaaa atatttactt cagggtctgc acttgtaagc cttgatgaat
    12961 aatatgagaa tgatataaaa tctactgtgt tctctttaag tatcttttta tcatcttctt
    13021 ccatcacgat atttattcct tttcgttcca attcttttaa tgcataagct ggatattctc
    13081 cacgagattg tacatctata aaaaagtagt tttccctatc tttttctata gatttccata
    13141 catctagtgg attacaagta ttggcataag tattccccgc tgctatcata caccctattt
    13201 taaattcttg atttatgtta tgagctagtt tagtaactaa tgcacttgca actaattgat
    13261 gatgtgcagc ttgatatttt atttgttctt cattttctcc ctcttcaata tagagacctg
    13321 cacctataaa tggtaaatga agtatcatat ttatttcatt aaatgttagc caatacttta
    13381 tcttatcttt aaatcttgta aaaataactt cacaatattt tacatataaa tctactagct
    13441 tacgattcct ccatgctcca tattttttta ctaaatacat tggaacatca aagtggcaga
    13501 ttgttactac tggttctatt ccatacttta aacactcatc aaacaaatct tcatagaatt
    13561 ttagaccctc ttcatttgga acttcctcaa ttcctgttgg aaatatccta gtccatgcta
    13621 ttgacatacg atagcattta aaccccatct cagcaaataa ttttatatcc tctttgtaat
    13681 tgtgatacat atctattgct tcatgagctg gataataatg ctcctcatca cattcaaaca
    13741 ttttcatttt cccagtcaaa actggaaatc tatcttttcc ctgtggtata acatcaacat
    13801 tagctagacc tctaccacct tctttatacc caccttcaca ttgatttgct gctgttgctc
    13861 caccccacaa aaaatctttt ctaagtgcca taactctacc tccaataatt tatttatagt
    13921 aaatttattt taaaataaaa atacatatat ctaattctca attaataaag ccgtccttta
    13981 cgctaaaaca gttatcaatt cctgactatt acttatatgc ttttgtgtat tttctacgat
    14041 atccacataa tcagaataat ttgtaacgac tactggagtt tctaagcaat atccttcttt
    14101 tgatatgctt tctatatcaa aagttagtaa tgtctgacct tttttaatct tatctccttg
    14161 attgacaaaa gatttgaaat acttaccttc taactgaata gtatttaacc ctatatgtat
    14221 caataattca caaccattat cagatataat tcctattgca tgttttgttg gaaataaagt
    14281 cattacagtc ccatcaaatg gtgctactac ttttccttct tttggtgtta ttgctacacc
    14341 ttttcccaat acacctgttg aaaatgctgc atcttgcata tgttccaaag cctttacttc
    14401 accttttatt ggactatata ttacttctct tttgtttttt tctacagcgc ctttactatt
    14461 attatctttg gaaatttcta ctatagtatc atctttccaa agaaagaatg ttaatacaaa
    14521 tcccaccaca atggctacta ttatagatat tgtcgttgca taaacacctg tcatatctcc
    14581 agttgttgga tttatataat taggtatacc aaatattcca agtccaccca ttacataact
    14641 ttttacattc attactccca ttacagctcc tgctacagca ccacctacac aactgtatat
    14701 gaatggtttt ttccttggta aagttattcc atatattgca ggttctgtta caccacatat
    14761 tcctgaaata attgcaggta tacacaactc cttcattttc ttatctttta atttaaagta
    14821 cattgctgcc actactgctg tttgtgcaaa agatggtaca aatgctccca ctaatattgt
    14881 gtcaaatccc atagtcataa ggtttgacat agctattggt actagtcccc aatgtagccc
    14941 aaatattaca agcacttgcc acaataaacc agttaatgcg ccacatagaa ttggtgaaaa
    15001 gttaaataaa gctaaaaatc catttccaac tatattagaa gcataggtag ctataggccc
    15061 tataaccata aatcctaatg gtaaagaaac tagtaaaact gccattggta caaagaaatt
    15121 ttttactaca tctggtatta ttttagcaaa cattttttct aacttagatg ctaagaatac
    15181 tattaaaatt actggaacta ctgttgaagt atagttcatt gctattactg gtaatcctaa
    15241 gaaagttata tatactggtg attcaaatat tgttcctgta aatagattgt ataaaggttg
    15301 acctgttgat gccagagtgt ccaattgaat acctgaatag cacaaaactg cacctataag
    15361 cataccgata aatggtttta gcttaaattt cttagcagct gtatatccaa gaattatagg
    15421 catatactgg aacattgcat caccaattgc atttaacata atatacgcac catcagtttc
    15481 tccatacaaa cccagtgctg taaatactgc attgaaacct tttatcatac cagcagcaca
    15541 cagtactcct agtattggtt ggaaaactcc tgatattatg tctataaact tattaagtaa
    15601 tccttttggt gaatctgaac ctgaattatc tattgtatct cctcctaatc cagccaaatc
    15661 acacacaact tcataaacat ctggaacatg gttaccaata actacttgat attgacctgt
    15721 acttttcatt acagtaacta ttccatccat tttttttagt acatcatcat ttgctattga
    15781 ctcatctttt aacttaaaac gtagtctcgt tatacagtga gataaactgc taatattatc
    15841 ctttcctcct acattctcaa tgatttcttt tgatagttgt tcatactttc tcatttttta
    15901 tcctccattt tctttttgaa atatgaaggt ttggccaact taatgtcacc acccaatata
    15961 tataaaccta attattagga tttatatctg atttaatact tttgcttacc accctttcga
    16021 catgaatagt taagtataat ttttcctcat ctgttaaatt gtaattgtat tttttatgta
    16081 taaattcatc taacttttct atgcatttat aagcattttt atatttgact tttattacct
    16141 ttaataaatc atcttcatta ttttcatgta aattattact gacaactctt tctgcaaagt
    16201 actttaagtg agtgataaat ctatgataat aaactgaatc ttcatcaaat actattctat
    16261 aatggtactt aaccacattt gcgatttctt gtattatttg cgtaatatca tatataattt
    16321 ttatactttg atccatttgt gcattaacta tatgtagtgc aataaatcct gcttcatctt
    16381 ctggcaatct aatttcaaat tttttttcta tataatctaa agcctttaaa cctatgttaa
    16441 attcattttt ataaaatctt tttatatccc aaagtaatac attttttaca tttactcctt
    16501 cacgagctct ttctattgca gtatacatat ggtctgttaa agaaatataa atactatcat
    16561 ttaatctttt ccctagtttt tctttagcat ataaaattat ttcatctgat aattccatat
    16621 atttcatagg aatatctgct attaattctt taaatttatc acatatattt ggatctgaaa
    16681 gtttgaatat cttatcaatc ttatcctttg atatatggtc tcctgtacgt tttttataag
    16741 ctaaacctct tcccataact actatttcct tatgattttc atcagttgta ataattacat
    16801 tattattgag tattttttca ataatcattt tgatgcctcc tttattagca ttaagttaaa
    16861 ataaatcttt tttaattatt tagaatattt aaaatgtaat ttattacata aaaaaaaacc
    16921 tatttattac acattgaata gactcccatg agccctgagt aataaatagg tttagcttgt
    16981 atttaaacaa tcactatccg tttgtatttt attttaatta ttatttatta tatgttagca
    17041 aatttatttt ttagtgtcaa ctttttctta atattttaat ttttaataaa ctatataaat
    17101 tgaatccaca aaaatattat ttttatcaat aatcaatata aaaaaaggta ttactttttt
    17161 taagtaatac caatttattt ttaatttaag tgattttttt taatctcatc aagtagtcca
    17221 tcttctaaat aaatttctaa tgcacatcct accattgcct ttactgcttt gtgaagtttg
    17281 tcataagctt cctcagaatt tgcatagctt agaaactctt tttcatgaac atataccatt
    17341 ggatttatat ctaaagctat ttctgtatac atagtaggac aaacttgact tacattccct
    17401 atgtctgtag aacctactgg accatcttta ccttctaata tatctgttat acctgcctca
    17461 atcaaattat tttttgttat cttttgaaga actttattat ttattatgtt gtcaaaagaa
    17521 ttttcaaaat atctataaga aagcttcgct cctgtcatta actctgcacc ttttgcacag
    17581 tttataacct ttttagttac ttcatctaaa taacttcttt cagcagctct tacaaaaaac
    17641 ttacaggctg ctttttctgg aactatattt ggagcttctc ctccatttga aactattcca
    17701 tgtattctaa catctgattt tacatgttgt cttagtgcat ttatcccagc aaaagtaagt
    17761 tgtactgcat ctagtgcgtt gactcccata tgaggatgac tagccgcatg tgctgatacc
    17821 ccttcaaaac taaactctaa tgaatctatt gccaaagcct tgcaatttat attattaaaa
    17881 gcttctatat gcatttgata acttacatca acatcatcaa aagctcctgc cttaactaaa
    17941 tcacattttc ctcctgttgt ttcttcagct ggtgttccta ttagtacaat ttttccttta
    18001 aaattttctt ttaacttact taatactata caagctccta gtgtagatgc tgcaatccag
    18061 ttgtgcccac aagcatgagc tggttcttta ttttctccat atccaggaag tgcatcatac
    18121 tcagctaaaa atgctatagt aggtccatca ttatttccta attcagctct aaaagcagtc
    18181 tccatattgc aatacggata aattgtatca aatccatatt cctccatttt ctctactaag
    18241 tattttgatg atatatattc ttcttcccct aattctggat tgttaaatat aaaattacaa
    18301 acttcttcaa tttcttttcc aagttcattc tctatctcat aagctctttt ctttatactc
    18361 tctatcataa cttacctcct actagaatcc tagatataat cctattgtca aaaataccat
    18421 tacaagaact gtcaatgcta ccattaaagg aattatgaat ctaacccact ttgaccaagg
    18481 aatattggct actgctagtg ctcccattaa aacacctgat gtaggtgtta ttaagttagt
    18541 tacacctata ccaaattgat aagctgtaac tatcatttgt ctagatacat ctactaagtc
    18601 cccaagtggt gcaagtacag gtattgtaag tgatgcaagt cctgatgctg atggtattaa
    18661 aaatgctatt aatccttcta ttaagaaagt taaatttata aatatagttt ttggtaatcc
    18721 gtttaataaa tttgctgcag aatttaaaat tgtatctatt ataaatccat tttctgctat
    18781 tattacgatt ccacgtgcaa atgctataca caatcctgct gtcattaaat ctccagctcc
    18841 acttataaat gaattaacta tatcatcttg tttaagacct gatgctatac ctgatattat
    18901 acctatagct gtaaatatca ttgcaatttc agttatatac catcccaact ttaaaactcc
    18961 atatatcata atcgccatac ctattgtgaa aattgctatt accatggctt ctctttttgt
    19021 aaattctttt atgtttgaat cactattaac cattgactct tgatttagag atatatcgtg
    19081 tactaaagat tttttagggt cttttttaac tttattggca tacatcataa caaaccctat
    19141 agatatagcc atatatatta taaacataac aactctaaac tctattccag aacctggagt
    19201 tatttgtgat aatgcttgtg ctattcccac agagaaagga ttagtagtag ctgctccaaa
    19261 tccagctgtt gctcctataa atactgtggc cactgctgtc aatgaatcat atcccatact
    19321 ggtcataagt ggaataaata tcatataaaa aggtagtgtt tcttcactca ttccaaatgt
    19381 tgttccacct agtccaaata gaatcattga aataggtatt attaaaaatt gaaacctctt
    19441 cattttattt acggtatgag ctataacagc ttctatagcc cctgttttat taactatccc
    19501 aaaagctcct ccaactatta aaacaaatcc aactacttca gctgcatcta taaatccgtt
    19561 tataggtgct gtaaatacat cagtaattcc ttgtggattt gatggtgctt tttcataagt
    19621 tccagctaca acaactgacc ttccatctat atctactctt tcaaaatttc cacttggtac
    19681 tatccaagta agaattgcca ttaacactat tagcaaaaat attattgtaa gtgtctgtgg
    19741 catagtaaac tttttctttt ttaaactgct catttttaat ctccccactt tttaaattaa
    19801 aatattttat tattgcatcc ctttatccta ttttgtatag atgtaaaaaa gctcaacatg
    19861 gatacatgtt gagctaaatg tgctaaatac aacatacctg catcttccct ttatcttgtt
    19921 taagatagct ctccatcaaa attcgggata attttgatga cagtactgta cctattaaat
    19981 acagtcccag cactagcttc ggacactagt acttcggcac ttactccttt tatattgcct
    20041 cattggtttc cgacctcttc aatattaatc ttagtaaatg caacctctac ctcaatcttt
    20101 ttattagatg aggtaattct tattaatttt tattaattca aagtaattgt agcataatat
    20161 gtcgagattg taaatatttt cagtataata attcttactt tatgatatga ttcaattttt
    20221 cacaaataat tttttctcca tataatttaa ataatgtagc atattttgaa tacacacaaa
    20281 ttttccatga atttcaaatt attttaatac ccttataata aaaaattaaa gccatgtaaa
    20341 cttatcaatt tacacggctt tatatacatt aatctacacc aagtaccgct ttgagttttg
    20401 atgctattgt aataactcgt ggtccaaata taacttgaac tgagtttttc tttttaacaa
    20461 ctccacttgc acccaattca tttacccaga aaccatcatc atagaatcca tcaattgaat
    20521 ttaactctac cctaagtcta gttgcacagt ttgtaacatt ttttatatta ctacttcctc
    20581 ctaaaccttc tattatagag tgtacaagct ctgtatcttt atcatctact tcatctccta
    20641 cagcattgtt atatactgtt ttaccagcat ctacagattg tttttctcta tattctttct
    20701 tgctataaag cttaacttct tctgaacttc ttcctggtgt ctttatatca aacttcttaa
    20761 ttgcccatgt aaatattata taagtaacta taaaaaatat tggtatcaaa aataataatg
    20821 gtaatgcatc cactttttgg ggttgcaata gattaggaaa catatttcta aatgattccc
    20881 ccattataga aacattaaac atttctgtaa atacatacgt caatcctgct agtggtacat
    20941 atgctaaaaa gtacaataat ggtgcaacaa ataagaatgt atactctatt ggttccgtta
    21001 ctcctaccaa catggctgtt actattgctg gtattaatat acttattgct tttttcttat
    21061 tgacattatc agccaactta tacatagcta aagctgctgc tggtaatatt gcaaaatgta
    21121 ttaataaacg tcctgttgtg aagttacgag ttatataaga tagtgtatct gggtctccca
    21181 tttgagctaa tcttatattg ttaacaccct cgtaaattgc tccacctact tccattgttc
    21241 caccaactct tgtatattct ataggaaatg caattaagtg atgtatacca aatggtaata
    21301 atgctttatc cattgctcca aaagtaaatg ttcctaataa tccactagaa cctatccaat
    21361 taccaagttt agataatgcc aaccctactg gtggccatat aaagtacatt gctgtaccca
    21421 atactattga tgctataaac atcataatca ttgaaaatct tggtcctgag aaaaaagata
    21481 atgcactagg aagttctatg tcaacaaatt tgttatgtac tgcactagct acaaatccag
    21541 ctattatagc tccaaatata ctacaatcat atgaaaatat tcccaagaaa gaaccatata
    21601 aggaacttcg actatatgct tctgcctcgc ctaatccaga tgccataaac gcttctgctg
    21661 taactgtatc tgaatttata ccatttgctg ctagcaatgt acttataact gtgtgcattc
    21721 ctataaataa aacaattcct cctaatgcac cccatccttt ttccttttta gataatccaa
    21781 aagctatacc tgtaacaaag aatattggta aatatctcat taccataaac cctaggtcgt
    21841 ttattacttt aaagaataca tttataaatg acccttcttt tattattccg ctaagcccta
    21901 atgcataaac gttttccata tttgcaaaag ctgaccctaa ccctataaat actcctgcta
    21961 caacaagtaa tataacagga atcatcattg ctccagaaag tacttgtaat ttatccttca
    22021 tagtattatt tcctccattt ttattgaaat gccaggctat ataatgcata tatatatact
    22081 tctattgctt tgtataatgt atctatactt atattttcat ttgcctgatg tgaatttgga
    22141 ttatctccct ctattgtggg tccaaatgca actccatttt ctaatactct tgcataagat
    22201 gcagctcctt ttgtaaaaag ttctgcctct ttttcaaaac cttgtttata tgcacttgat
    22261 aatttattta ttaattcact atctggttct acatacagta attttaaatt cttatatata
    22321 tcaattatta aattttcttt ttttccaatt tcattaattc tattagttat aaactccaaa
    22381 cttataccct taggatatct aaaatctatt ttcatattca attcattttt atctaagaag
    22441 atactcatta tacatattgt agaaacgccc atatctacat cttctttata tatccctatt
    22501 ttttttccat gattatcatc tgtaaagtat gaatttaaaa tatcctttat tataactcct
    22561 ttatcgttta gccctttaat tctctctaaa tcttttgcta aactaaatgc acagttatat
    22621 gacctgtgtg gatttctcga caaagctctt tctcctgtat atcttactaa tacttttcct
    22681 tcttctactt cttctatttc tgtataataa gctaatgatt ctactaaatc tttcttatta
    22741 ttagttttaa agacagcctc aattttgtca catacaaatc catcttcttt gtttgacttt
    22801 atatcaacta aattgtgatt tctaaattta tctttatcta ttttctttct tagattgaag
    22861 tataaaatac ctttttctcc atttactatt gggaagtttc catctggaga aaatgcaaat
    22921 tttggctgtt catttacact aaaataatgt tccatacatt cccaagtcgt ctcctcagct
    22981 cctcctacta tcaatctaat ctttctcttt ggcttttcat taagttctct taagaaaagt
    23041 aatgcatata aaatccctat caaaggtcct ttgttatcat taactcctct accataaaga
    23101 tagttatctt tttgacatac tttaaaaggt ttactcttcc aaagttcttt attatatatt
    23161 ggcaccacat ctatatgatt aagcacccct actacttctt ctccttctcc atattctata
    23221 tgaattgcat atccatcaaa atcttttaca acaaaatctt tgtcttttgc tattttaata
    23281 agcttatcga aggcatttct tatttctatc ccaaatggtt gatttatatc tgttgtactt
    23341 tcatctctaa ttgaaggtat ttcaactaaa ctcctaatat cctttatgat attttcttga
    23401 ttggatttta aataatcaat aattagttct ttatttacta tcatcttaat tatttatcac
    23461 ccctttatat tagttttaca aagcatttca gttcatattt acattataaa ttttgagtat
    23521 tattttttca atatttcaaa tacttctcta aattaccata atgatacggt aa
//
LOCUS       DAFNNB010000046        23201 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0046, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000046 DAFNNB010000000
VERSION     DAFNNB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 23201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23201
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0046"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..443)
                     /gene="nrdR"
                     /locus_tag="L3M60_001579"
     CDS             complement(<1..443)
                     /gene="nrdR"
                     /locus_tag="L3M60_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HBN6226256.1"
                     /translation="MQCPYCNYKESKVIDSRHTDLKSIRRRRECESCKKRFTTYEKIE
                     TTPLMVIKKDNSREYFDREKIKYGLLKACEKRPVSIEEIESIVVHIENEINKCFIEEI
                     ETKKIGEMVMDKLKDLDEVAYVRFASVYRQFKDINTFVNELKSIL"
     gene            complement(465..728)
                     /locus_tag="L3M60_001580"
     CDS             complement(465..728)
                     /locus_tag="L3M60_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HBN6226257.1"
                     /translation="MIRVSDIMEKEIINVKNGKRMGFIIDIDMDIHEGKVVSFTIFGD
                     GSRSFFSRGGDEEVIFWNDILKIGCDTIIVNIGSELSLDEFEI"
     gene            complement(832..1605)
                     /gene="sigG"
                     /locus_tag="L3M60_001581"
     CDS             complement(832..1605)
                     /gene="sigG"
                     /locus_tag="L3M60_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HBN6226258.1"
                     /translation="MQVNKVEICGVNTSELPVLKNKQMKELLLQIKNGDEEARQQFVR
                     GNLRLVLSVIKKFNNRGENIDDLFQIGCIGLIKAIDNFDLSQNVRFSTYAVPMIIGEI
                     RRYLRDNNPIRVSRSLKDIAYKALQVRERLIRTNSKEPTVSEIAKELELEVESVVMAL
                     DAIQDPISLFDPVYQDNGDAIFVMDQVQDKKDTDENWLQEISLKEAIKKLNSREKLVL
                     DLRFYKGRTQIEVADEIGISQAQVSRIEKNALKNMRKYV"
     gene            complement(1697..2419)
                     /gene="sigE"
                     /locus_tag="L3M60_001582"
     CDS             complement(1697..2419)
                     /gene="sigE"
                     /locus_tag="L3M60_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HBN6226259.1"
                     /translation="MLRLKERIISFITMLGIKLKIIKPKGIYYMGGANILPPPLKPEE
                     EMELLQKLETDESVKSILIERNLRLVVYISRKFENTGIDVEDLISIGTIGLIKAVNTF
                     KLNKNIKLATYASRCIENEILMYLRKNNKKKTEVSFDEPLNIDLDGNELLLSDVLGTE
                     NDEIYKIIEEEIDRDLLVMALDRLSDREKQIMELRFGLIDKGIEKTQKEVAGMLGISQ
                     SYISRLEKKIISRLQKEMKKFV"
     gene            complement(2444..3316)
                     /locus_tag="L3M60_001583"
     CDS             complement(2444..3316)
                     /locus_tag="L3M60_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HBN6226260.1"
                     /translation="MFKGFKGGYMVYIEYYVIENLVINYIIISCTSILTKRYNPKKNK
                     LLGASLGGAYSVAYLYPSMNILFTIPFKIIIMSIITLISFRSKSKKDYVHVALVFYLV
                     NIFISGSTYFIIYFTGISHLKISFLIACTYVSCELLKYIYKDLKMLKYIKELTKTINI
                     KLLEKSFCCKALVDSGNLLKDPISQSDVIIVKSSVLEGFIPEKLLNINYEDIDMKKAQ
                     EIIGSLDDKTSGRVRIIPYKHAGSNKTSIIIGLKADYIEVDEQKIGNIILGISNFNDR
                     EYGAIMNPNILPGF"
     gene            complement(3615..4889)
                     /locus_tag="L3M60_001584"
     CDS             complement(3615..4889)
                     /locus_tag="L3M60_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBN6226261.1"
                     /translation="MIKLKKVLSLVMVGILSVSILAGCSKKEDSKGKEGKEKLVVWSA
                     PLTDHDADAWKPIFEKFEKENNCEIEFQIVPWDNYAEKYATAISAGEGPDIGYMYAEM
                     FPQFIEMGAVEDLTPYLEKSGTSDNYLYLDDAKMMGGIYGLPIEAANPGVLYYNKDIL
                     EKLGEKPPKTWDDFKRICEKATKDTDGDGKIDQWGLAQGWGSKVFGSLNWNWYPYLWQ
                     AGGGIYNDDLKSVKFNNAEGLKAANFINDMKKYIPEDALSKDSNEMIESVFGPGKAAF
                     TIMLSSAATSTFDKSFPDLNWGYVTSLEDKTVGSFASVDDLTLMSACKNKELGFKLLE
                     YMLSKESMEAFHKEVPRAPVAKGEKYQGDPRFEEMVTRDKHMYRPLTVGPHGVEIYEY
                     LWKQLQMMIAGDITPEQALNESAKYANGLLAQ"
     gene            complement(5174..6331)
                     /gene="ftsZ"
                     /locus_tag="L3M60_001585"
     CDS             complement(5174..6331)
                     /gene="ftsZ"
                     /locus_tag="L3M60_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBN6226262.1"
                     /translation="MLNFDVELEECAQIKVIGVGGGGNNAVNRMVEAQLKGVEFISVN
                     TDKQALYTSKAEYKVQIGEKLTRGLGAGANPEVGKRAAEESKDEIVKLLQGADMVFVT
                     AGMGGGTGTGAAPVVAGLAKEMGILTVGVVTKPFAFEGKIRMKNAEGGIAELKSKVDT
                     LITIPNDRLLQIVQKNTSMLDAFAVADDVLKQGIQSISDLIAVEGLINLDFADVTTIM
                     KDKGLAHMGIGSASGETRAIDAARQAIQSPLLETSIQGAKGVLLNVTGGPNLGLFEVN
                     EASTLVMESCDPEANVIFGASIKEDLGDEIMITVIATGFEGLQNGALDLETKPKSSIR
                     SSLNTTVKQAVKEIEEEVIAEEKIEPPKKASIIEEDDDESMEIPTFLRRRR"
     gene            complement(6521..6874)
                     /locus_tag="L3M60_001586"
     CDS             complement(6521..6874)
                     /locus_tag="L3M60_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic family protein"
                     /protein_id="HBN6226263.1"
                     /translation="MLWVLGGLAFGVVIGYYIPFTYSMDYSIYMSVAILAIMDSIFGA
                     IKSNFEDNYDNVIFITGFIGNAILAILLTYIGEKLGISLYYVAVLVFGMRLFNNLSII
                     RRYIISNIMNKKRKN"
     gene            complement(6973..7647)
                     /locus_tag="L3M60_001587"
     CDS             complement(6973..7647)
                     /locus_tag="L3M60_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBN6226264.1"
                     /translation="MKKKIVILGAFILGIFISTYIKSLDPNRVYITLGQTENIESEVE
                     NTKKEIKNLKETLKNNKKTLNEYKEAKKNGNESIQTLMQKELEEVKELSGYSSVTGPG
                     ITITMKDSERELKDGQNPNDLIIHDIDILRVLNDLKKAGARAISINGERVLATSKIKC
                     SGATITVNDTTYGQPFIIKAIGDIDLLSAAINSPESYAKSLKDIYGINIKVQENNGNK
                     VNLFTK"
     gene            complement(7652..8350)
                     /locus_tag="L3M60_001588"
     CDS             complement(7652..8350)
                     /locus_tag="L3M60_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HBN6226265.1"
                     /translation="MKKRFINKSIIIGSIVLGVLVSLQLKIINIENRGMTTFKRGEQL
                     TEELKSLKKEKENLQSEVNSVKKDIETYRGKNNEGEQDILKSEIEKYETLAGYTDVEG
                     TGIKIEISSTKDLGNSDKNDSILYNYDLLLSMINKLNSAQASAISINDQRIVSNTYMY
                     LKEDKLYMNDTVIKEPIIIKAIGDSDTLASALNIKYGIVWEIETYYNAKVKVTKEDNI
                     EINGHSDINKAEGK"
     gene            complement(8373..9113)
                     /locus_tag="L3M60_001589"
     CDS             complement(8373..9113)
                     /locus_tag="L3M60_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HBN6226266.1"
                     /translation="MKKRRKLNTNNVMIVLVFILMLSFCICIIVKSDLFDVKKIDVIG
                     NKRVTKSNIMKELNINLNENIFAYNFKDMKNKLIKNPYIENVEIKRKLPNKIIISLKE
                     KEIFAVLKDEDNYCYIDKKGNLLEELRGSNESKKDLIVDVDYSVDDNKSIKFKNYKTK
                     ENVFKTLNYLKEEGIYRKINYVNLKKESNIEMLTRSNIKILLSNDDNLDYNISRVSKI
                     LIDLQNKNTNGGTINLNYGKLAVYSPEG"
     gene            complement(9374..10600)
                     /gene="murG"
                     /locus_tag="L3M60_001590"
     CDS             complement(9374..10600)
                     /gene="murG"
                     /locus_tag="L3M60_001590"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBN6226267.1"
                     /translation="MKVLLSGGGTGGHVYPAIAIANKIRDEHPDAEIIFVGTEKGIES
                     EIVPKYGFELKTVTVQGFKRKIDFDNVKRVFKLFKGLEQSRKIVKKFKPDVVIGTGGY
                     VSGPVLFNASMGKIPAIIHEQNSFPGVTNKILSKTVTKVLTSFEDSHKRFPEAAEEKL
                     VFTGNPVRKEILLSRKNIARKNLSISEEKRMVLCYGGSGGSRKINDAMRLVIKNMVNE
                     DIAFIFATGKSYYDEFMESISDINLKPYQKVVPYLEDMANALAASDLVIGSAGAISLA
                     EITALGKPSIIIPKAYTAENHQEYNAKSIEKQGAGIAILEKNLTPESLNTAVFKLLGD
                     RELLVDMANASKTIGKPEAIDLIYDEIMKVYNSTQKSTSKKTKKEKVIKEVKEIKKET
                     TPSIEGQAKVIGIKKR"
     gene            complement(10618..11748)
                     /gene="spoVE"
                     /locus_tag="L3M60_001591"
     CDS             complement(10618..11748)
                     /gene="spoVE"
                     /locus_tag="L3M60_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HBN6226268.1"
                     /translation="MPKENLKKQIDIRMKTEFDGVVFYTTMLLVFVGIVMVFSASFIQ
                     SSFKHNDAYYFLKKNVIYAVLGFIVMIITSRIDYSFWKKNATAIGAIAVVLLLLVLTP
                     LGIEANGAKRWLGIGALTFQPAEIAKFATIILTAKLIERNYDKIKSLTKGVVPLLVVP
                     GIFFALIILQPNLSTAGTVILVTFVMIFVAGMDMKIVFAMIGSGVALFAALVIAEPYR
                     LSRVTSFLDPFQDPLGKGYQVIQGLYALGSGGLFGLGLGKSKQKYFYIPEPQNDFIFA
                     IIGEELGLIGCIIVIMLFVVLVYRCVRIALKTSNVFACMVVIGIGAQIGIQAALNIAV
                     ATSSMPATGVALPFISYGGTSLTIFMGAVGIVLNISKHVKIN"
     gene            complement(11800..13155)
                     /locus_tag="L3M60_001592"
     CDS             complement(11800..13155)
                     /locus_tag="L3M60_001592"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBN6226269.1"
                     /translation="MDLKGKKVLLVGLAKTGISTIKHLDKLGASIIVNDIKDENKLRN
                     ILDELKSINDIKYILGHHPEDIDDIDMVVVSPGVPLDLPFILKLKNSGKYIIGEVELA
                     FKLSNNPIFIGITGTNGKTTTTSLVGEIFSRAKRDTYVVGNIGNPVIDTIETSNEESV
                     LVTELSSFQLESIDEFRPKVSAILNITEDHLNRHHTMEKYIEAKTNIFMNQTSEDFCV
                     LNYDDEIVKGLADKCNAKVIYFSRTKKVNGGVYLENNDIIIDIDDKIKFLNKDDVSLP
                     GGHNLENCMAAIAIAYVCKIDLEVIKDVLMTFKGVEHRQEFVRNLDNVIYVNDSKGTN
                     PDSTIKAIQSYDRPIILIAGGMDKGSNFDELLETAKSYVKSLVLLGETASNIENCAKN
                     KGFNDIHIVKDMEEAVKTSYEISKSGDIVLLSPACASWDMYESFEVRGKDFKDNVNNL
                     K"
     gene            complement(13174..14142)
                     /locus_tag="L3M60_001593"
     CDS             complement(13174..14142)
                     /locus_tag="L3M60_001593"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBN6226270.1"
                     /translation="MMLGITELTYTALIAFLIVIIIGPIFIPMLRKFKFGQTVRDDGP
                     QTHLAKNGTPTMGGIIMIVAILITGLTRVKVSHDMAVGLICIAGFGFIGFLDDFIKIK
                     LKRSLGLKAYQKIILQVALSFYVAFYQYTSSSSASQLMIPFTDFVINVGILYIPIMMF
                     IIVAIVNAVNLTDGLDGLASGVTLIVSVFFMLFASSIAGNTEVAVLAAATVGACLGFL
                     GFNSYPARVFMGDTGSMALGGAVVAFSVLTNSVLIIPIIGGIYFAEALSVLIQVGYFK
                     ATRKRFFKMAPIHHHFEQCGWPETRVVFIFWIVTVVLAWISIIAVF"
     gene            complement(14185..15558)
                     /locus_tag="L3M60_001594"
     CDS             complement(14185..15558)
                     /locus_tag="L3M60_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBN6226271.1"
                     /translation="MECLTIKELVLATNGKLIYGDYNDCVSDIVIDSREANAQNAFVA
                     IVGENLDGHTFMKSAYDSGCKTFIKNESNGIKLESSDINLIEVKDTSLALGNISKYYK
                     EKFDIPFIGVTGSVGKTTTRDMIYSAISAKLNILKNEKNLNNHFGVPLTLFNLSKEHE
                     CAVIEMGMSGFNEIKYLVDIVNPKIAVISNIGLSHVEKLGSQEGILKAKMEITSNFDE
                     ANTLIVNGDDKFLSTLKEKEHVYKLKTFGFNKNNDIYCESYTMEEDSLTFICVINGKK
                     EEIFIPTIGEHNIYNAMSAILVGLCLNISLDYIKKGLKNFKGTKMRLDIIKNEKLTII
                     NDSYNASPDSMDAALKILGRYKGRRVAILGDILEMGEISEYGHRLVGKSSMNNTDIII
                     TIGENSVFIGEEAKQLGFNSANIYHFENREDVFNKLNELVKTGDTILVKGSRGMRLEK
                     IVEYLNK"
     gene            complement(15725..17704)
                     /locus_tag="L3M60_001595"
     CDS             complement(15725..17704)
                     /locus_tag="L3M60_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein D"
                     /protein_id="HBN6226272.1"
                     /translation="MRKVKRISKKRLVLVLILACALFFCLVIRTGYLQLMKGNWLSTK
                     ALEQQTRDIPIEPKRGTIYDRNMKELAVSVTKYTVWCKPVEVEDKKEAAEKVAEILDE
                     DYKDIYALISKKNMALVKVKRWIDDDKASKIRDAKLSGIWVAEDNQRYYPYGNFAPYV
                     LGHTSSDATGISGIEMQYDKKLKGKPGKLIVSTDASGREIPQGMEKYYEPVQGNGLVL
                     SIDEVIQHYTEKAVQKAYELNNAKKVTAIAMNPKTGDILALASKPDYDPNDSRTPIYP
                     YYQEELEKYNDKDKIKGYYQMWRNPAVSDTYEPGSTFKLITSSSALEEGVIKDGEKFT
                     CTGSVTVGGRKIKCWRHYRPHGTQEFKQAVQNSCNPVFVELGSRLGVGKMYDYIESFG
                     LMDKTGIDLPGEAKGILYNEKNVGPVELATISFGQSISVTPIQLITAISSIANGGDLM
                     QPRVVKSYTDNKGNITETVKPKKVRSVISKETSKKMLEIAESVVTEGGGKIAYIPGYR
                     LGGKTGTAQKVIDGKYAPGKYICSFVGIAPCDDPQIVVLAIVDEPTGVSAFGSTTAGP
                     IVKEIMNDSLKYLGVKPVYKEEEKAEYEKKQVKVPDVRNLKIGDAVKALEDVKLKPDL
                     DADIELPEDTKVKDIFPKPGVKVNEDSSITLYFEN"
     gene            complement(17716..18063)
                     /locus_tag="L3M60_001596"
     CDS             complement(17716..18063)
                     /locus_tag="L3M60_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226273.1"
                     /translation="MKKNYKIHNLNEYKNKKKNNYKKRNKKKIGTKCILLIATFAVII
                     LNLCGYSKISEMKYDIYYLQKDLRKKEIVLEELKAKETEKTSIKEIELKAKELLNMDY
                     PKDGQIKYIDVDN"
     gene            complement(18540..19475)
                     /gene="rsmH"
                     /locus_tag="L3M60_001597"
     CDS             complement(18540..19475)
                     /gene="rsmH"
                     /locus_tag="L3M60_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBN6226274.1"
                     /translation="MEFHHVSVLLNECIENLNIKPDGVYVDCTMGGAGHSKEIVKKLS
                     DKGLFIGFDQDKNAINTAKERLSEYEGRVKFVHSNFENIKEELEKIGVYKIDGVLADL
                     GVSSHQLDEADRGFSYMQDAPLDMRMDVRCEFSAYDVVNTYTEDELTKIIKDYGEDNW
                     AKRIAKFIVEERANKTIETTGELVDIIKKAIPKKARIDGPHPAKRTFQAIRIEVNNEL
                     GVITKMINDASSIMNEGGRICIITFHSLEDRIVKNAFKYLASDCICPQHLPICQCDKE
                     SEVKIITRKPILPSEEEVEANPRSRSAKLRVAEKI"
     gene            complement(19505..20251)
                     /locus_tag="L3M60_001598"
     CDS             complement(19505..20251)
                     /locus_tag="L3M60_001598"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBN6226275.1"
                     /translation="MDRVAFTLFGIDIMWYGILMACGMILGTLIAIKESKRVGIKEDD
                     VLNIAIIAIPAGLICARIYYVVFNWSYYAQNMSQIFNFRGGGLAIHGGLIGGILAGYI
                     YTKIKNINFLKMADTVILGMPLAQAIGRWGNFINGEAHGGATNLPWGIMVDGVKVHPT
                     FLYESIWDFGIFIVLLLFRKNKKYEGQVIVTYITLYSIGRFFIEGLRTDSLMLGPLRM
                     AQVISLIGVIGGLIAHIYLSKKNKNNISEE"
     gene            complement(20650..21936)
                     /locus_tag="L3M60_001599"
     CDS             complement(20650..21936)
                     /locus_tag="L3M60_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBN6226276.1"
                     /translation="MEKIVNDILKEEVYYEKLQNGLDVYFMPKRGFMKKYAILATNYG
                     SNDLEFVPLGEDKKIRVNEGIAHFLEHKMFEQPDGGDAFDKFSKLGVNANAFTNFTMT
                     AYLFSATENFYESLEHLIDYVQTPYFTDENVEKEKGIIAQEIKMYNDDPDWNVYFNCL
                     KAMYVNYPARIDIAGTVDSIYKITKEELYKCYNTFYNPGNMALFVVGDLDVEKVIDVT
                     KKSNNYKVDKLSKSIERFYPEEPKSVKEKEVIEKFPISMPMFNIGFKDSNVGLKGKEL
                     LRKEIVTDILVGMLFKKGSKLYEDLYMQGLINDNFGAGFSSQVDYAFSIIAGDSKEPK
                     KVKKIILDYIEKSKKEGLSKEEFDRTKKKKIGSFIKCFDSINFIGNSFISYVFKDINL
                     LDYLDVIKDITFEEVEERLKEHFKEEYCVISIVEPK"
     gene            complement(21951..23165)
                     /locus_tag="L3M60_001600"
     CDS             complement(21951..23165)
                     /locus_tag="L3M60_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBN6226277.1"
                     /translation="MIKTEKFKSNLISLYVQRLLDEKETTKNALIPSIIASGSAKYPS
                     ARAISNKLDDLYGSSMGADAVKRGERQVLSFKVINISEKYLDESIFEEVVEFFNEVIN
                     NTLVVDGGFKEEYLNIEKENLREKIQSIINDKKEYAQDKCIEAMCKGERYSISEFGYE
                     DEIDSITSKELYEHYKNILKTSPIDIVVEGNFDEDKVVDIISKNLKFEREEIIDIPRA
                     DFIKNVDEVKVVDEQMEITQGKLVMGYRANVDYADTDRYYALVVGSNVLGGGPHSKLF
                     VNVREKESLCYYIFSSIEKYKSILFISSGIETKDYDKAVELIKEQVESVKAGDISDEE
                     LENSKLALVNSMKSITDNIGGMSDFAFSQSMAKTNSEVQDIISSIEKVTKKDIVEAIK
                     NIELDTVYFLRN"
BASE COUNT         7100 a         4354 c         2309 g         9438 t
ORIGIN      
        1 atcaatatac ttttaagttc atttacaaaa gtatttatat ctttaaattg tctatacaca
       61 gaagcaaatc ttacataggc aacttcatct aaatctttca atttatccat taccatctca
      121 ccgatttttt tggtttcaat ttcttctata aaacatttat ttatttcatt ttcaatatgt
      181 acaacaatgc tttcaatctc ttcaatagaa acaggtcttt tttcacatgc ctttaacaat
      241 ccatatttta tcttttctct atcaaaatat tctctactat tatccttttt gattaccatt
      301 aaaggagttg tttctatttt ctcatacgta gtaaacctct ttttacatga ttcacactca
      361 cgtcttcttc tgatagattt taaatctgta tgtctagaat ctattacctt tgattcttta
      421 taattacaat aaggacattg catctatatt cctcctttgt ttaactatat ctcaaactca
      481 tctaagctta attctgaacc tatatttact attattgtat cacaacctat tttaagtata
      541 tcattccaaa atataacttc ttcatcccct cctcttgaaa aaaaacttct acttccatct
      601 ccaaaaatgg taaatgagac tactttacct tcgtgaatat ccatgtctat gtctattata
      661 aaccccatcc tttttccatt ttttacattt ataatttctt tttccattat atcagaaact
      721 ctaatcatat tgtactcacc ctttctttaa aaataaatat gtatcgaagt aaaaaaaaat
      781 gacctatata ggtcatttaa attaaaataa tgtttacagt ccatggatta cttatacata
      841 ttttctcata ttttttaaag cattcttttc aattcttgat acttgtgctt gagatatacc
      901 gatttcatca gcaacttcta tctgagttct tcctttgtaa aaccttaaat ctaataccaa
      961 cttttctcta ctatttaatt tcttaatagc ttctttaagt gatatttctt gcaaccaatt
     1021 ttcatcagta tcttttttat cttgaacctg atccataacg aatattgcat ctccattgtc
     1081 ttgatacact gggtcaaaca gagatatcgg gtcttgtatg gcatcaagtg ccattacaac
     1141 cgactctact tctaactcta attcctttgc tatttctgat actgtaggtt ctttagaatt
     1201 tgttctaatt aatctttctc taacttgtag tgccttatat gctatatctt ttaatgacct
     1261 acttactcta attgggttat tgtctcttag atatcttcta atttctccta taatcatagg
     1321 aacagcataa gttgaaaatc ttacgttttg acttaaatca aagttatcta tagcttttat
     1381 aagaccaata caacctattt gaaataaatc gtctatattt tctcctctgt tgttaaactt
     1441 ttttattaca ctcaagacta atcttaaatt ccctcttaca aactgttgtc tggcttcttc
     1501 atcaccattt tttatttgta ataaaagctc cttcatttgc ttatttttaa gaactggaag
     1561 ttctgatgtg ttaacgccac atatttcaac cttattaact tgcatatatt ttcagtcctc
     1621 tctcaaaagt attcatctat tgaaattatt tacatttcca atttttttat actttttaag
     1681 aagaattaat ttaacattat acaaattttt tcatttcttt ttgcaacctt gaaattattt
     1741 tcttttcaag tctagatatg taggactgtg atattccaag cattcctgca acttcttttt
     1801 gtgttttttc gataccttta tcaataagac caaatcttaa ttccataatt tgcttttctc
     1861 tgtcacttaa tctgtcaagt gccataacca acaaatctct atctatttct tcttcaatta
     1921 ttttgtatat ttcatcattt tctgtaccca atacatcaga taataaaagt tcatttccgt
     1981 ctaaatctat atttaaaggt tcatcaaatg atacttctgt tttcttttta ttattttttc
     2041 ttaagtacat taatatttca ttttctatac atctcgaagc ataagttgcc aattttatat
     2101 ttttattcag tttaaaagta ttaactgctt ttatcaaacc tatagttcct attgaaatca
     2161 aatcttctac atctattcct gtattttcaa attttctcga tatgtagact actaatctaa
     2221 gatttctttc tattaaaatt gactttacac tttcatctgt ttctagcttt tgtaaaagtt
     2281 ccatttcttc ttctggtttc aatggaggag gaagtatatt tgcccctccc atatagtata
     2341 ttccctttgg ctttattatt tttaatttaa tacctaacat agtaataaag cttataattc
     2401 tttctttcaa tcgtaacatt ccttgtcccc cttaaatttt cacctaaaat ccaggtaata
     2461 tatttggatt cattattgct ccatattctc tatcattaaa atttgaaata ccaagtatta
     2521 tatttcctat cttttgttca tctacttcta tataatcagc ttttaaacct attattatgc
     2581 ttgtcttatt gcttccagca tgtttataag gtattattct aactctacct gaagttttat
     2641 catctaaact tcctataatt tcttgtgctt ttttcatatc tatatcttca taattaatat
     2701 ttaatagttt ttctggaata aatccttcta gcacacttga cttaactatt attacatcac
     2761 tttgacttat tgggtctttt aacaaattac cactatctac taaagcctta cagcaaaaac
     2821 ttttttctag taattttata tttatagtct ttgtaagctc cttaatgtac ttaagcattt
     2881 tcaaatcttt ataaatgtat tttaataatt cacaactcac ataagtacat gcaattaaaa
     2941 aagatatttt taagtgactt attcctgtaa aataaattat aaaataagtg cttccagaaa
     3001 taaaaatgtt tactagataa aaaactaaag ctacatgaac ataatctttt ttgctcttgc
     3061 ttctaaatga aattaaagtt attatactca tgattattat tttaaatggt atagtaaata
     3121 gtatattcat tgatggatac aaataagcaa cagaataggc acctcctaaa ctagccccca
     3181 acaacttatt ttttttagga ttatatcttt tagtaagaat agaagtacaa cttataataa
     3241 tataatttat tactaggttt tctataacat agtactcaat atacaccatg tatcccccct
     3301 taaaaccttt aaacatatta taagatagta tgtacaaaat tttatgtcac ttttgatggt
     3361 cgaatccaaa acaaattcta tttaaaaaga acaatgatat atgtcttata ctttatgtcg
     3421 ccaaaagata tttttttaat gccaaacaaa taggcttttt tgtagaatcc aatatatcta
     3481 ggtagaagtt attcgttcta cttttggtat atttccataa aaaaaagact acataattta
     3541 ataatatatc aagtaaatct tttacttatc taaaattaaa ttatgtagtc tgtaaattca
     3601 aatataaatt taaattattg tgctaataat ccatttgcat attttgcaga ttcatttagt
     3661 gcttgttctg gagttatgtc tccagctatc atcatttgaa gttgtttcca taagtattca
     3721 tatatctcta ctccatgagg tccaactgtt aatggtctat acatatgttt atctcttgta
     3781 accatttctt caaaccttgg gtctccttgg tatttttcac ctttagcaac tggagctcta
     3841 ggtacttctt tgtggaaagc ttccattgat tctttgctca acatgtattc aagaagttta
     3901 aatccaagtt ctttattttt acatgctgac attaatgtta aatcatctac tgaagcaaaa
     3961 cttccaactg tcttatcttc taatgatgtt acatatcccc agtttaaatc tgggaatgat
     4021 ttatcaaatg tacttgtagc agcagaagac aacattatag taaatgcagc ttttcctggt
     4081 ccaaatacac tttcaatcat ttcattacta tcttttgaca aagcatcttc tggaatatac
     4141 tttttcatat catttataaa gtttgcagct ttcaaacctt ctgcattatt aaatttaact
     4201 gattttaaat catcattata tatacctcca ccagcttgcc ataaatacgg ataccagttc
     4261 cagtttaaac tcccaaatac tttagaaccc caaccttgtg caagacccca ctggtctatt
     4321 tttccatctc catcagtatc ttttgttgct ttttcacata ttcttttaaa atcatcccat
     4381 gtttttggag gtttctctcc taatttttct agtatatctt tattgtaata caatacacct
     4441 ggattagcag cttctatagg taaaccataa ataccaccca tcatttttgc atcatctaag
     4501 tatagataat tatctgaagt tcctgatttt tctagataag gtgtcaaatc ttctaccgca
     4561 cccatttcta taaattgagg gaacatctct gcatacatgt atcctatgtc tggaccttca
     4621 cctgcactta ttgctgttgc atatttttct gcgtagttat cccatggtac tatctgaaat
     4681 tcaatttcac agttattttc tttttcaaat ttttcaaata tcggcttcca tgcatctgca
     4741 tcatggtctg ttaatggtgc agaccacaca actaattttt ccttaccctc tttgccttta
     4801 gaatcttctt tttttgaaca ccctgcaaga attgatactg acagaatccc taccataact
     4861 aaagaaagaa ccttttttag tttaatcatg cttaatcccc tttcagactt taatttgtct
     4921 ttttcctcaa atgtttacat ttaattcaga tttaatatta acaaataaga ctactcttgt
     4981 ctaatcacaa aaaactatac taactaccat aactatattt tttttctata catattagat
     5041 ttaatttaca ctaaaaaact tactttttaa attttgtcaa tatttaaaag tgtttttaat
     5101 cctagccaca aactaactat ttaaattgag caaaataaaa agaagtctat tgacttcttt
     5161 ataagcttac atattatctt cttcttctta agaatgtagg tatctccata ctctcatcat
     5221 catcttcttc aattatactt gcctttttag gaggctctat tttttcctca gcaattactt
     5281 cttcttcaat ttcttttaca gcttgtttta ctgttgtatt taatgaggaa cgtatgcttg
     5341 atttaggctt agtttctaaa tccaaggcac cattttgtaa tccttcaaat cctgttgcga
     5401 ttactgtaat cattatttca tcacccaaat cttcttttat agaagctcca aatataacat
     5461 ttgcttctgg gtcacaggat tccataacta aagttgatgc ctcattaact tcaaataatc
     5521 ctaggttagg accacccgta acattaagaa gaacaccttt agctccttgt attgatgttt
     5581 ctagaagtgg agattgaata gcttgtctag cagcatcaat tgctctagtt tcaccactag
     5641 cactacctat acccatatga gcaagtccct tgtctttcat tatagtagta acgtctgcga
     5701 agtccaagtt tattaaccct tcaactgcta taaggtctga aattgattgt ataccttgtt
     5761 ttaatacatc atctgcaact gcaaaagcat ctaacattga tgtatttttt tgtactatct
     5821 gtaaaagcct atcatttggt attgttataa gagtatctac ttttgattta agctctgcta
     5881 ttccaccttc agcattcttc atccttatct taccttcaaa tgcaaaaggt ttagtaacta
     5941 cacctactgt aagtatcccc atttcttttg ccaagcctgc aactactgga gcagctcctg
     6001 taccagtacc accgcccatt ccagctgtta caaaaaccat atcagctcct tgaagcaact
     6061 ttactatttc atctttactc tcctcagcag ctctttttcc tacttcagga tttgctcctg
     6121 ctccaagtcc tctagttaat ttttctccta tttgaacttt atattcagct tttgatgtat
     6181 ataaagcttg tttatctgta tttactgata taaattcaac gccttttagt tgagcttcta
     6241 ccattctatt gacagcattg tttccaccgc cacctacacc tattacttta atttgtgcac
     6301 attcttctaa ttctacgtca aagtttagca tcataaaccc ccttctctca tgattcaaag
     6361 ttatcgtatg tattaattat atcattttct tcttttattt tgttaacctt taagtaacat
     6421 attccacaaa aacgtaaatt ttccttttta aatttcttac ttatattgtc aaatattact
     6481 ctttaagtat aaaataattt acgtatatat taaatgctct ttaattttta cgttttttat
     6541 tcataatatt actaataata tatcgtctaa ttatagataa gttattaaac aatctcatac
     6601 caaaaactaa tacagcaaca taataaagag aaattcctaa cttttctcct atataagtca
     6661 ataatatagc caatatagca ttacctatga aacctgtaat aaaaattaca ttatcatagt
     6721 tatcttcaaa attggatttt atcgctccaa aaatagaatc cattattgca agaatagcta
     6781 ctgacatata tatggaataa tccatagaat atgtaaatgg aatatagtaa cctattacta
     6841 cgccaaatgc taatcctcct aaaacccata acataaatct tcacctcttt attttacttt
     6901 gaaacacaac tacattacaa tcctttttgt taattttttt tactttttct ttattttttt
     6961 gtatttttcg ttttattttg taaataaatt cactttattt ccattatttt cctgcacttt
     7021 tatattaatt ccatatatat ccttaagaga tttagcgtat gattctggag agtttatagc
     7081 agcactcaat aaatctatat ctcctatagc ttttattatg aatggttgtc catatgtagt
     7141 atcatttact gtaatagtag ctcctgaaca ttttatctta ctcgttgcta gtactctttc
     7201 cccatttata gaaattgctc ttgctccagc cttttttaaa tcatttaaaa ctctcaaaat
     7261 atctatatca tgtataatca aatcattcgg attctgtccg tcttttaact ctctctcact
     7321 atccttcata gtaatagtta tgcctggtcc tgtaacactt gaataaccac ttaattcttt
     7381 aacttcttca agttcttttt gcataagtgt ttggatagat tcattaccat tcttttttgc
     7441 ttctttatat tcattcaaag tttttttgtt gtttttaagc gtttctttta aattttttat
     7501 ttcttttttt gtattttcta cctcactttc aatattttca gtttgaccta atgttatgta
     7561 tactctattt gggtctagtg attttatata agttgatatg aaaatgccta atatgaatgc
     7621 acctaatata actattttct ttttcatact gttatttccc ctctgctttg tttatatcac
     7681 tatgaccatt tatttctata ttgtcttcct tggtcacttt tactttggca ttataatatg
     7741 tttctatttc ccagacaata ccatacttta tattaagtgc agaagccaat gtatcagaat
     7801 caccaattgc ttttattata attggttctt ttataacagt gtcattcata taaagcttgt
     7861 cttcttttaa atacatatat gtattagaaa ctattctctg gtcgtttata gatatggcac
     7921 ttgcttgtgc tgaatttaat ttatttatca ttgacaataa taaatcataa ttatatagta
     7981 tactatcatt tttatcagag tttcctaagt cttttgtaga acttatctca atttttatac
     8041 ctgttccttc tacatctgta tatccagcta atgtttcata tttttctatt tctgacttaa
     8101 gtatgtcttg ttcaccttca ttatttttgc ctctataagt ctctatatct ttcttcacac
     8161 tattaacttc tgattgtaga ttctcttttt ctttttttaa agatttaagt tcttctgtga
     8221 gttgctctcc tcttttaaaa gttgtcatcc ctctattttc tatattaata attttaagtt
     8281 gtaaactaac aagaacacct aatacgatac ttcctatgat tatactttta tttataaatc
     8341 tttttttcaa tatatcactt ccttacaatt gtctatcctt ctggactata cactgccaac
     8401 ttcccataat taagatttat agtacctcca ttagtatttt tattttgcag gtcaatcaaa
     8461 attttactta ccctagatat attataatct aagttatcat cattagaaag taagattttt
     8521 atattacttc ttgtaagcat ttctatattg ctttcttttt ttaagtttac ataatttatt
     8581 tttctataaa tcccttcttc ttttaaataa tttaaagttt taaatacatt ctccttagtt
     8641 ttataatttt taaattttat agatttatta tcatcaacac tataatctac atctactatc
     8701 aagtcttttt tactttcatt agaacctctt agttcttcta acaaattgcc ttttttatct
     8761 atataacaat aattatcttc atcttttaat actgcaaata tttctttttc ttttaaactt
     8821 ataattatct tattgggaag ttttcttttt atttccacat tttctatata tggatttttt
     8881 ataagtttat ttttcatatc tttaaaattg tatgcaaaaa tattttcatt tagatttata
     8941 tttagttctt tcattatatt tgatttagtt actctcttat ttccaattac atctattttc
     9001 tttacatcaa ataaatcact ttttacaatt atacatatac aaaaagataa cataagtata
     9061 aaaactaaga ctatcatgac attattcgta tttaattttc ttcttttttt caaatataat
     9121 caaccccttt ggttaataaa tacaccttta gagcttattt ttcctacaaa aaaattataa
     9181 tatttaaagt caaatttttg cattttatta cataacatcc aattaaagta ttaaatctta
     9241 taagtaaatc aatatttcat caaaataaat atagtatgct atatatagtt aaatccgtat
     9301 atatacaaat aaacccctaa tttagaataa tttagaaaat ctaatatatt ataaattagg
     9361 ggtaaatatt tacttatctt ttctttattc ctattacttt agcttgacct tctatactcg
     9421 gagtcgtttc tttttttatt tcttttactt ccttaataac tttttctttt tttgtttttt
     9481 tagaagttga cttttgtgtt gaattgtata ctttcattat ctcatcatat ataaggtcta
     9541 tggcttcagg cttacctata gttttacttg catttgccat gtcaaccaaa agttctctat
     9601 cccctaaaag tttaaatact gctgtattta ggctctctgg agttagattt ttttctaaaa
     9661 tagcaattcc cgcgccttgt ttttctatac tcttggcatt atactcttga tgattttcag
     9721 cagtataagc tttaggtatt attatagatg gctttccaag agctgttatc tcagctaatg
     9781 aaattgctcc tgcactacct ataactaaat cacttgctgc aagtgcattt gccatatctt
     9841 ctaagtaagg tacaaccttt tgatatggtt taagatttat atcgctaata ctttccataa
     9901 attcatcata atagcttttg cctgtagcaa atataaaagc tatatcttca tttaccatat
     9961 tctttattac aagtctcatt gcatcattta tttttcttga acctccacta ccaccatagc
    10021 aaagtaccat tcttttttct tcacttattg atagattttt tcttgcaata ttttttcttg
    10081 ataataaaat ttcttttcta actgggtttc ctgtaaaaac taatttttct tctgctgcct
    10141 caggaaatct tttatgtgaa tcttcaaaac ttgtaagtac ctttgtaact gttttagata
    10201 aaatcttatt agttacacca ggaaaagaat tttgctcatg tattatggct ggtattttcc
    10261 ccatagatgc attaaataaa acaggtccac taacataacc accagttcct ataactacat
    10321 ctggcttaaa tttcttaaca atctttcttg attgctccaa acctttaaaa agcttaaaaa
    10381 ctctttttac attatcgaaa tctatttttc gcttaaagcc ttgaactgtt acagtcttta
    10441 attcaaatcc atacttagga actatttctg actctattcc tttttccgtc ccaacaaaaa
    10501 ttatctcggc gtctggatgc tcatctctta ttttgttcgc aatagctata gctggataca
    10561 cgtgccctcc agttccaccg cctgataata aaactttcat caaatcatca tctcctgtta
    10621 attaattttg acatgctttg agatgtttaa cactattcct acagctccca taaagatggt
    10681 gagtgaagtt cctccataac ttataaatgg tagtgctaca cctgtagctg gcattgatga
    10741 tgttgcaact gctatattta aagctgcttg tattccaatt tgtgcaccta tacctataac
    10801 aaccatacaa gcaaatacat tagaagtttt caatgctatt cttacacatc tgtagactaa
    10861 aactacaaac agcataataa ctattataca accaatcaat cctaactctt ctcctattat
    10921 agcaaatata aagtcatttt gcggctctgg tatataaaaa tacttttgtt tactctttcc
    10981 taagcctaga ccaaacaatc cacctgaacc tagtgcatat agaccttgta ttacctgata
    11041 ccccttacca agagggtctt gaaaagggtc taaaaatgat gtaacacgac taagtctata
    11101 tggttcagca attactaatg cagcaaataa agcaacccca ctccctatca tagcaaatac
    11161 aattttcata tccattccag caacaaatat catcacaaaa gtaactagaa ttacagtacc
    11221 tgctgttgac aaatttggct gaagtattat caacgcaaaa aatatacctg gaactactag
    11281 aagtggtaca acaccttttg tcaaagattt tattttatca taatttcttt caattaattt
    11341 tgcagttaat attattgtag caaactttgc gatttctgca ggttgaaatg ttaaagcccc
    11401 tattcccaac catctttttg ctccatttgc ttctatccct aaaggggtca aaactaacaa
    11461 taataatact actgctatag ctccaattgc tgtagcattt ttcttccaaa aactataatc
    11521 tattctcgat gtaataatca taactataaa tccaagcaca gcatatatta cattcttttt
    11581 tagaaaatag tacgcatcat tatgtttaaa tgaagattgt ataaaacttg cactaaacac
    11641 cataactatt cctacaaata ctaaaagcat agtagtataa aatactactc catcaaattc
    11701 agtcttcatc cttatgtcaa tttgtttttt caaattttct ttaggcatcc aactaatcta
    11761 cctccattct tccatttaag taagtttaga ggtaagagat tattttaaat tgtttacatt
    11821 atctttgaag tcttttcctc ttacttcaaa actttcatac atatcccaac ttgcacaagc
    11881 tggtgaaaga agcactatat ctccgctttt tgaaatttca taagaagtct taactgcttc
    11941 ttccatatct tttactatat gtatgtcatt aaatccttta tttttagcac aattttctat
    12001 atttgatgca gtttctccta atagaaccaa tgatttaaca tatgactttg cagtttcaag
    12061 aagttcatca aaattacttc ccttatccat ccctcctgct ataagtatta taggtctatc
    12121 gtaagactgt atagctttta tagtagaatc tggattagtt ccttttgaat cattaacata
    12181 tattacatta tctaaatttc ttacaaactc ttgtctgtgt tccactcctt taaatgtcat
    12241 aagtacatcc tttataacct ctaagtcaat tttgcataca tatgctattg ctatagctgc
    12301 catacagttt tctaagttat gacctccagg taaacttaca tcatctttat ttagaaattt
    12361 gattttatca tctatatcta ttattatgtc attattttct aaatatactc ctccatttac
    12421 ttttttagtt cttgaaaagt aaattacttt agcattgcat ttatcagcta gaccttttac
    12481 tatctcatca tcataattta atacacagaa atcctcagaa gtttgattca taaatatatt
    12541 tgtctttgct tctatatact tttccatagt atgatgtcta tttaagtggt cttctgtaat
    12601 atttaatatg gcacttactt taggtctaaa ttcatctata ctttcaagct gaaaactact
    12661 taattcagtt actagcacag attcttcatt tgaagtttct atagtatcta tcacaggatt
    12721 tcctatatta ccaactacat atgtatctcg ttttgctctt gaaaaaattt ctcctaccaa
    12781 actagttgta gttgtttttc catttgtacc tgtaattcct ataaaaattg gattatttga
    12841 tagtttaaaa gctaattcta cctcaccaat tatatacttg cctgaatttt ttaatttcaa
    12901 tatgaatggt aggtctagtg gtacacctgg agacactaca accatatcta tatcatctat
    12961 atcttctgga tgatgtccta atatgtattt tatatcattt atagatttta attcatcaag
    13021 tatatttctc aatttatttt catctttgat atcatttact attatacttg cacccaattt
    13081 atctaaatgt ttaattgtag atataccagt ttttgcaagt cctactaata aaactttttt
    13141 tccttttaaa tccatttttt attcccccca gccctagaat actgctatta tacttatcca
    13201 agccaaaaca actgtaacaa tccaaaaaat aaatacaact cttgtttctg gccaaccaca
    13261 ctgttcaaaa tggtggtgta ttggagccat cttaaagaat ctctttcttg ttgctttaaa
    13321 atatccaact tgaatcaaaa ctgatagagc ctctgcaaaa taaattcctc caattatagg
    13381 tattataagc actgagttag ttagaactga aaatgctaca actgcacctc ctaatgccat
    13441 tgaacctgta tcccccataa acactctagc tggatatgaa ttaaatccta aaaaacctaa
    13501 acatgctcca acagttgctg ctgctaaaac tgcgacttct gtatttccag caatagaact
    13561 tgcaaatagc ataaagaata cagaaactat taatgttact cctgatgcta atccatctaa
    13621 tccatcagtc aaatttacag catttactat tgctacaatt ataaacatca ttattggtat
    13681 atataatatt ccaacattta ttacaaaatc tgtaaatggt atcataagtt gtgaagcact
    13741 tgatgatgat gtatattgat aaaaagcaac ataaaaagat aatgcaactt gaaggattat
    13801 tttttgatat gcttttagtc caagtgatct ttttagcttt atttttataa agtcatctaa
    13861 aaaaccaata aaaccaaaac ctgcaataca tataagacct actgccatat catgactaac
    13921 ttttactctt gtaagtccag ttattaaaat tgcaacaatc attataattc cacccatagt
    13981 aggtgttccg tttttagcta gatgtgtttg aggtccatca tctctaactg tctgaccaaa
    14041 tttaaatttt ctaagcattg gtataaatat tggtcctatt ataattacta ttaaaaacgc
    14101 aatcaatgcg gtgtacgtaa gctctgttat tcctaacatc ataaaactcc tctcccttgt
    14161 caattaactc tcattagttt aagcttattt atttagatat tcgactattt tttctaatct
    14221 catgcctctt gagcctttta ctaaaatagt gtctcctgtt tttactaatt catttaactt
    14281 gttaaataca tcttctctat tctcaaagtg atatatattg gctgaattga atcctaattg
    14341 ttttgcttct tctccaataa acactgaatt ttcacctata gttattataa tatcagtatt
    14401 attcatagat gattttccaa ctaatctatg accatactct gaaatttcac ccatttctag
    14461 tatatctcca agtattgcta ctcttctacc tttatatctt ccaagtatct ttaaagcagc
    14521 atccatagaa tctggactag cattatatga atcattaatt attgttaatt tttcattttt
    14581 tattatgtct agtctcattt ttgtcccttt aaaatttttc aaaccttttt ttatataatc
    14641 taaagaaata tttagacata aaccaactaa tatagcagac attgcattat atatattatg
    14701 ttctcctatt gttggtataa atatttcctc ttttttccca tttattacac atataaatgt
    14761 taaactatcc tcttccatag tataactttc acaataaata tcattatttt tattaaagcc
    14821 aaatgttttt aacttatata catgttcttt ttcctttaat gtagataaaa acttatcatc
    14881 tccatttaca attaaagtat ttgcttcatc aaagtttgaa gtaatctcca tctttgcttt
    14941 caaaatcccc tcttgtgaac ctaatttttc tacatgtgat aatcctatat tcgatatgac
    15001 tgcgatttta ggatttacta tatccactaa atattttatt tcattgaatc cagacatacc
    15061 catttctatt acagcacact catgttcttt acttagatta aataaagtca atggaactcc
    15121 aaaatgatta tttaagtttt tttcattttt tagtatattt agctttgcag atattgcaga
    15181 ataaatcata tccctagtag tagtttttcc cacactacct gtaacaccta taaacggaat
    15241 atcaaatttt tctttatagt attttgaaat atttcctaga gccaaagatg tatcttttac
    15301 ctctattaaa tttatatccg aactttctaa ttttattcca ttactttcat tttttataaa
    15361 agttttacat ccactatcat atgcagactt cataaatgta tgcccatcta aattttctcc
    15421 aactatggct acaaatgcat tttgggcatt tgcctccctg ctatctataa ctatatcact
    15481 tacacagtca ttataatctc catatattaa cttcccatta gttgcaagta ctaactcttt
    15541 tattgttaag cactccatta tcaaactcct cctttaaaac cttagctata ttctcttagc
    15601 aacaaaaggc aatgtatacc gttatatata cattgcctac gataactctt aactttataa
    15661 ttatatacta tatttctgtt tttttgaaga tgtttatggg tatatgttaa ttatctgtta
    15721 ctttttagtt ttcaaaatat agggttatac ttgagtcttc attaactttt actccaggtt
    15781 ttgggaatat atcctttacc tttgtatctt ctggaagttc tatatctgca tctaagtctg
    15841 gttttaactt cacatcctcc aatgccttta cagcatctcc tatttttaga tttcttacat
    15901 ctggcacttt aacttgcttc ttctcatact cagctttttc ttcttctttg tatactggtt
    15961 taactcctaa atattttaaa gaatcattca ttatttcttt cactattggt cctgctgtag
    16021 tactaccaaa tgcgcttact cctgttggtt cgtcaacaat agcaagaact actatttgtg
    16081 ggtcatcaca aggtgctatt ccaacaaatg aacatatata ttttcctgga gcatacttac
    16141 catctataac tttttgagct gtacctgttt taccaccaag tctataccct ggtatatatg
    16201 ctatctttcc tccaccttca gttacaactg attctgcaat ttctaacatt tttttagatg
    16261 tttctttaga tataacactt cttacttttt ttggttttac tgtttcagtt atatttcctt
    16321 tattgtcagt ataagatttt accactcttg gttgcattaa atctccacca ttagctattg
    16381 atgatatagc tgttataagt tgtattggag ttactgatat agattgacca aatgatattg
    16441 tagccaattc aactggtcct acattttttt cgttatacaa tattccctta gcttctcctg
    16501 gtaaatctat acctgtctta tccataagac caaatgattc tatataatca tacattttcc
    16561 caacacctaa tctgcttcct aactcaacaa atactgggtt acaagaattt tgtactgctt
    16621 gtttaaactc ttgtgttcca tgaggtctgt aatgtctcca acatttaatc tttcttcctc
    16681 caacagttac actacctgta catgtgaatt tttctccatc tttaattact ccttcttcaa
    16741 gagcactgga agatgtaata agcttaaatg tagaacctgg ttcatatgta tcacttactg
    16801 ccggatttct ccacatttga taatatcctt ttattttatc tttatcatta tatttttcaa
    16861 gttcttcttg ataatatgga tatattggtg ttcttgaatc attagggtca taatctggtt
    16921 ttgatgctaa agcaagtata tcaccagttt tagggttcat agctattgca gtaacctttt
    16981 ttgcattatt taactcatac gctttctgta ctgctttttc agtatagtgt tgaattactt
    17041 catctataga caacacaaga ccattacctt gtacaggctc ataatatttt tccattccct
    17101 gtggtatttc tcttcctgag gcatcagtac ttactataag ctttccaggc tttcctttta
    17161 attttttatc atattgcatt tctataccag atatacctgt tgcatctgaa gaagtatgtc
    17221 ccaacacata tggtgcaaaa ttcccatatg ggtaatatct ttgattatct tctgctaccc
    17281 atattccact taatttagca tctcttattt tacttgcctt atcatcatct atccatcttt
    17341 ttacttttac aagagccata ttttttttac ttattaaagc ataaatatct ttataatctt
    17401 catccaaaat ctcagctacc ttttcggcgg cttccttttt atcttctact tccacaggtt
    17461 tacaccaaac agtatactta gttacactta ctgctaactc tttcatattt ctatcatata
    17521 tagttcctct tttgggttct attggtatat ctcttgtttg ctgttctaga gcttttgtac
    17581 ttaaccaatt tcccttcata agttgtagat acccagtcct aataacaagg caaaaaaata
    17641 atgcgcatgc tagaataaga acgagtacca gccttttctt acttatcctc tttacttttc
    17701 tcaaatctat tccccctagt tatccacatc tatatacttt atttgtccat cttttggata
    17761 gtccatattg agcaattcct tagcctttag ctcaatttct tttatcgatg ttttttcagt
    17821 ttcttttgcc tttagttcct ctaatactat ctcttttttt cttaaatctt tttgtaaata
    17881 ataaatatcg tacttcattt ctgagatttt tgaataacca catagattca atattattac
    17941 tgcaaaagtt gcaatcagta aaatgcattt agttcctatt tttttcttat ttctcttttt
    18001 ataattattc ttttttttat ttttatattc atttagatta tgtattttat aatttttttt
    18061 caaattatca cctcgatagt atatagttta aattttcctc tatactatat ttgttgttaa
    18121 tcatatttct tcctctaaaa tttcatcgct aaattaatac actattgcac aatctaattg
    18181 tgtttttttc tatacacaac aaaaacctat aaattcactt atacttttat atgaatctat
    18241 aggttagata ttaattttta tttattttta tcacttctaa tattttcaga taatttcttc
    18301 cataaatata atatgtaact aactatctac tttctaattt ctccattata ttacttaata
    18361 tcatctatat ctatattttt atacctaaat tttcattata tatctcatta gcaaaaaaca
    18421 tttcttatag tgttaattat tttgtaatta ctttaccagt gcaacaaaat atacgatact
    18481 ttttaattaa ttataacaaa tcttatttta tacataagat tactctttaa ctttataaat
    18541 tatatttttt ctgctactct tagcttagca cttcttgaac gtggatttgc ttctacttct
    18601 tcctcactag gtaatatagg ttttctagtt attattttta cctcagattc tttgtcacat
    18661 tggcatatag gtaaatgttg tggacatatg caatctgatg ctagatactt aaatgcattt
    18721 ttcactattc tatcttctaa tgaatgaaat gttattatac atattcttcc accttcattc
    18781 attatagaag aagcatcatt tatcatcttt gtaattactc caagttcatt attcacttct
    18841 attcgtattg cctgaaaagt tcttttagct gggtgaggac catctattct ggctttttta
    18901 gggatagctt ttttaattat atctactaac tctcctgtag tttctattgt tttgtttgct
    18961 ctttcttcaa ctataaattt agctatacgt ttagcccagt tgtcttcacc ataatctttt
    19021 attatcttag ttaattcatc ttctgtgtaa gtatttacaa cgtcatatgc agaaaactca
    19081 cacctaacat ccattctcat atcgagtggt gcatcttgca tataagaaaa tcctctatca
    19141 gcttcatcaa gttgatgaga tgaaactcct aaatcagcta atacaccatc aatcttgtaa
    19201 actcctattt tttctaactc ctcttttata ttttcaaaat tgctatgaac aaacttcact
    19261 ctaccttcat attcactcaa tctctctttt gctgtgttta tagcattttt atcttggtca
    19321 aaccctataa ataatccctt atctgaaagt ttttttacta tttccttaga atgtcctgct
    19381 cctcccatag tacagtcaac atacactcca tctggcttta tatttaaatt ctctatacac
    19441 tcatttaaaa gaactgatac atgatgaaat tccatagtta cctcccttaa attcactttt
    19501 taaactattc ttcactaata ttgtttttat ttttctttga taaataaata tgtgcaatta
    19561 gaccaccaat aactccaatt agacttataa cttgtgccat tctaagtgga cctaacatta
    19621 aactgtctgt tcttaatccc tctataaaaa accttcctat tgaatataaa gttatataag
    19681 taactataac ttgaccttca tattttttat tttttctaaa tagtaataaa actataaata
    19741 ttccaaagtc ccatatggat tcatacaaaa aagttgggtg tacctttact ccatcaacca
    19801 ttattcccca aggtaaatta gtagcacctc catgtgcttc tccattaata aaatttcccc
    19861 atcttccaat agcttgtgct aagggcattc caagtataac tgtatcagcc atttttaaga
    19921 agttaatatt tttaatctta gtgtatatat aaccagctaa tattccacca attaaccctc
    19981 catgtatagc aagtccacca cctctaaaat taaaaatttg agacatattt tgagcatagt
    20041 aactccaatt gaatacaaca taatagattc ttgcacagat taatccagct ggaattgcta
    20101 ttatagctat atttaataca tcatcttctt taattcccac tctttttgat tcttttattg
    20161 ctattagagt tcctaaaatc ataccacatg ccataagtat tccataccac atgatgtcta
    20221 tgccaaacag tgtaaatgct actctatcca taacgtcacc tctttttatt aattaaaaat
    20281 atttccaaag aaatatccta taattacaat taattgtaat tatactaaat tatgataaca
    20341 cattaatgtt aattctacaa aaaatattaa aatttattct atagatacct atactatatt
    20401 ataccaatct aattgatata aaattctttg aactgaacct cacatgacta aaatcacaag
    20461 tgtgcataga tattttttaa tcaaaaagat tgatgtatgt atacaccaat cttttatata
    20521 tcttatctat aactcttaaa ttttttaact caacttctat aaagatattt cataaatttg
    20581 ttttactaaa ataaacattc aaaaacataa actaacattt aactatttat tcttattatg
    20641 attaaatttc tatttaggct ctacaataga aataacacaa tactcctctt taaaatgttc
    20701 ttttaatctt tcttcaactt cctcaaaagt aatatcttta ataacatcta aataatccaa
    20761 taaatttata tccttaaaca cataagatat aaaactattt cctataaagt ttatagaatc
    20821 aaagcactta ataaatgaac ctattttttt ctttttagtt ctatcaaact cttcttttga
    20881 taagccctct ttttttgatt tttctatata atcaagaatt atttttttta cttttttagg
    20941 ttctttagaa tctcctgcta ttatgctaaa agcataatct acttgagatg aaaatccagc
    21001 tccaaaattg tcatttatta aaccttgcat atataaatct tcataaagct tacttccttt
    21061 tttaaataac attcctacaa gtatatcagt tacaatctct ttacgtaaaa gttcttttcc
    21121 ttttaaacca acattactat ctttaaaacc tatgttgaac ataggcatag atatagggaa
    21181 tttttctatg acctcttttt cttttacact ttttggttct tctggataaa acctttctat
    21241 actcttagag agtttatcta ctttgtaatt gtttgatttt ttagttacat ctataacttt
    21301 ttctacatct aggtcaccaa caacaaatag agccatattt cctggattat aaaaagtgtt
    21361 gtaacattta tataactctt ctttagttat tttatatata ctatcgactg tacctgctat
    21421 atcaattcta gcaggataat ttacatacat tgcctttaaa caattaaagt atacattcca
    21481 atcagggtca tcattataca tttttatctc ttgtgctatt ataccttttt ccttttcaac
    21541 attttcgtct gtaaaataag gtgtttgaac atagtcaatt agatgttcta aactctcata
    21601 aaaattttca gtagcagaaa ataagtatgc tgtcattgta aaattagtaa atgcatttgc
    21661 atttactcct aatttagaaa atttatcaaa tgcatctcct ccatcaggtt gctcaaacat
    21721 cttatgctct agaaaatgtg caataccttc attcactcta atttttttat cttctccaag
    21781 tggtacaaac tctaaatcat tcgaaccata attagtagcc aaaatggcat actttttcat
    21841 aaaacccctt ttaggcataa aatatacatc taatccattt tgcaatttct cataataaac
    21901 ttcttctttc aatatatcat taactatttt ttccatagac atctcctcca ttaatttctc
    21961 aagaagtata cagtatcaag ttctatattt tttatagctt caactatatc tttttttgta
    22021 accttttcta tactagatat tatgtcttgt acttcagaat ttgtctttgc catagactga
    22081 gagaaagcaa agtcagacat accacctata ttatcagtta tagatttcat agaattaact
    22141 aaagcaagtt tactgttttc taattcttca tcagatatgt cacctgcttt tacactttct
    22201 acttgttctt ttatcaattc cacggcttta tcataatctt tagtttctat accagatgat
    22261 ataaacagga tacttttata cttctctatt gaagagaata tatagtaaca taaactttct
    22321 ttttctctta cattaacaaa taatttagag tgtggaccac cacctaatac attgcttcca
    22381 actactaaag cataatatct atctgtatct gcataatcca catttgctct ataacccata
    22441 actaacttac cttgagttat ttccatttgt tcatctacaa cctttacctc atcaacattt
    22501 tttataaaat ctgctcttgg tatatctata atttcttctc tttcaaattt tagattttta
    22561 cttattatat ctacaacttt atcttcatca aaattacctt ctacaactat atctataggt
    22621 gatgttttaa gtatgttttt ataatgttca tataactctt ttgaagttat actatctatt
    22681 tcatcttcat aaccaaattc acttatacta tatctctcac ctttacacat agcctcaata
    22741 catttatctt gtgcatattc ctttttatca tttattattg attgtatctt ttctcttaaa
    22801 ttctcttttt caatatttaa atattcttct ttaaatccgc catctacaac aagcgtatta
    22861 tttatcactt cattaaaaaa ttctactact tcttcaaata tactctcatc taaatacttt
    22921 tcacttatgt taattacttt aaaactcaat acttgacgtt ctccacgttt tacagcatca
    22981 gctcccatag atgaaccata taaatcatct aatttattgg atattgctct agcactagga
    23041 tatttggcgc ttccacttgc tattattgat ggtattagag cattttttgt agtttctttt
    23101 tcatccaata gtctctgaac atataaactt ataagattcg atttaaactt ttctgttttt
    23161 atcaaagtaa gattaatatt attacctaaa tttattattt t
//
LOCUS       DAFNNB010000047        22898 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0047, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000047 DAFNNB010000000
VERSION     DAFNNB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22898
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0047"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            351..2540
                     /locus_tag="L3M60_001601"
     CDS             351..2540
                     /locus_tag="L3M60_001601"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HBN6226278.1"
                     /translation="MKYIIKRDGTKELFSKSKIEDAVYKASINSEGGVDRDLAYDISR
                     KIANDYNSMEKCLSVEEIQDIVEVLLMESNRKDIAKHYILYRERRSEIRTKAWEFDEL
                     QKSIWKNKYRHNGESFDEWIKRISNNSSRISKLIRQKRFLFAGRILANRGLQKEGKKV
                     TYSNCYVIEPPKDSIEDIFDTAKKLARTFSYGGGVGIDISKLRPKGAKVNNAAKHTTG
                     AVSFMELYSMTTGLIGQRGRRGALMISMDVNHPEIEEFIDIKTDLEKVTKANISVRVT
                     DEFMQAVREKKLYNCTFEVETTGEKIVKIVNAYDLFNKFVKNNWDYAEPGILFWDKIK
                     TYNLMSEDKNFCFSGVNPCAEEPLPSGGSCLLGSINLSEFIIQPFGEHATFDINKLKS
                     CVREAVVALNDVLDEGLNLHPLQEQRDSVHKYRQIGLGVMGIADMLIKMGIRYGSDES
                     LELCDLIGKTMLNEAVKQSALIAKEYGTFSEYKYENISKSKFFIENLNEDTKEMVKKY
                     GLRNSQLLTIPPTGSISTMIGISGGIEPIFNLSYIRKTESLHDEDVYYKVYTPIVKEY
                     MDKKGITDEECLPDFFVTAMNIGYEERINMQKVWQKYIDASISSTINIPFETTVEDVF
                     NIYIKAWEAGLKGVTIFRDGCKRSGILINEKKEENNKENKKENSKKEYYKNNKKEKDN
                     KDEKAKEIINKNFEEDEKFVCPECGSEGIAHTGGCSICLKCGYSGCN"
     gene            2608..3375
                     /locus_tag="L3M60_001602"
     CDS             2608..3375
                     /locus_tag="L3M60_001602"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBN6226279.1"
                     /translation="MQDKSVREIKEIIETLEVEKYMEYIELLRVDERKSVQGLAIKLA
                     KKLDNIRKEEERLETINIFENEGYNKGYLYIGGIDEAGRGPLAGPVVASVVVFKKDTK
                     IEGVNDSKKLSEAKRDELFEVIKEEALDYGIGIVNNEEIDEFNILNATYMAMKKAINC
                     LKKAPDYLLVDAATIPGIDIAQNPIVKGDSKSISIAAASILAKVTRDSIMYQYDRVYP
                     EYGFKSHKGYGTKEHYEAIEKYGITPIHRKSFLKNIL"
     gene            3433..4449
                     /locus_tag="L3M60_001603"
     CDS             3433..4449
                     /locus_tag="L3M60_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBN6226280.1"
                     /translation="MEYNELLKNARENLNGSCKVCKVCNGVACAGEVPGMGGKGSGSS
                     FIENSKSLEKVKVNMRVIHDVSNPDTSIEMFGKKMSAPIFAAPVTGTTLNMGGKINER
                     DYIEPVVAGCANSGIYAMVGDTAVDAFLMENLDVVKKYNGAGIVFIKPWDNENIIKKI
                     RLAEEAGAFAVGVDIDACGLVTLSLHGKPVLPKNVEQIKELVKSTKLPFILKGIMTVE
                     DALMAVEAGVDAIVVSNHGGRVLDCTPGACEVLPKIADAVKGKVTILADGGVRTGLDV
                     LKMIGLGADAVLIGRPFVTASFGGATDGVETYVNKLQSELSSSMILTGCQTIKDIDGK
                     VIYK"
     gene            4516..4764
                     /locus_tag="L3M60_001604"
     CDS             4516..4764
                     /locus_tag="L3M60_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226281.1"
                     /translation="MSRKAYEEALVELEKFIDERKEIIKSAEDCIDKYIVDRTLPFDY
                     KDKCVEWQQELLDIAEAQVLEANELSVLLQEKKELEED"
     gene            complement(4937..5434)
                     /locus_tag="L3M60_001605"
     CDS             complement(4937..5434)
                     /locus_tag="L3M60_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HBN6226282.1"
                     /translation="MYKIGESVMYPKEGACCVNDIVTKKINHQMQKYYELSVIFNSNL
                     KISIPVLNADRIGIRPVMDGNDVDKFIQSIDKTDGVWVFDRKQRLKLYHDKFHSGDVF
                     EIVKLIKMLMIQDCSKQLCSTDKEFLNKAQRFALSELAAAQCKSYTVVLEEMKKHILN
                     SKSKN"
     gene            complement(5678..6742)
                     /locus_tag="L3M60_001606"
     CDS             complement(5678..6742)
                     /locus_tag="L3M60_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226283.1"
                     /translation="MIRLTVANIKRYLKNHTLLINMVMLPIILIFSLNFFINNSGNQS
                     MYFSPVAIVSDSSGKYEHKLINSSKLKENSFRLNEQDKAMNLLKNNKVSAVFVLDKNF
                     SNSIDNLQRPVVKCFKIENGGGSLWAESQIESFITKSLKLKMDKNIDDKLTKTNIIDN
                     KLDKNKGSFLVVFLICYFMYINAAHLASDLFTLKKSNVLKRLISTDNKDIKIIFSIFL
                     GLFFIQSIVYTFTLLCFSIIEDFNLSLNVLMIVLANSFVSTGFVFWIARVFKNESSIS
                     LISTFYSLIGLAISLSSLIPSMDKLSFMANLSKLTPFYWTIDAIKNNGSILINIIILI
                     LIGTIFVTAGSLKLRDFAKN"
     gene            complement(6742..7842)
                     /locus_tag="L3M60_001607"
     CDS             complement(6742..7842)
                     /locus_tag="L3M60_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226284.1"
                     /translation="MKLYMSVKTMLKGLKSSFILNLIYFLALPLILSWFLGMVTESMF
                     QNPIKTESTPIVIYDKDNTRLSNHLTKYLKNDLSYILTVKKDDSKAELKLTIPKGYES
                     SLLNEKSNTLNIEKLGTRDDIAILLQDILDTYHEKFYLNNSQKISSEDFSKLFNKNSI
                     DTSIIGNNVKQSSYEYFALVSLGFLVIIFIMNNILSNYISESKGLSKRLYSMPITRVQ
                     FLIYDFVGLWIYSFIFLLLYVLFFRIIGITFKGNFAILLLLCALSSYFMTSISTFVNS
                     FFSKKYGTIIVYALLFLQTIFGGIFSMISDAFTKLTSLSPTYLIGELFSNYETFKTID
                     SIGNLIFTCLITSTILIALAIVKEKYKWREVQ"
     gene            complement(7856..8791)
                     /locus_tag="L3M60_001608"
     CDS             complement(7856..8791)
                     /locus_tag="L3M60_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226285.1"
                     /translation="MDIVRVNNITKRFNDKLALDNISFSVKKGEIFGLIGPNGAGKST
                     LINIITNLMLPNSGSIQINGLDLSKDYIKAKSIIGLVPQELAIIETLTPFDNLEYFGA
                     FYGLKGNLLKERINEALEVTGLTEVKKKKVKKLSDGMQRRLNIGIALLNHPKILILDE
                     PTVGVDPQSRNHIFNFIKDMSKKYETTVIYTSHYMEEVEHLCSKIFIMDEGKEIAFGD
                     NAYLKSLVSTNTKLIMEIKNINAQLIFDLKNTKGVISILENNSLLELDIDKKLQLTEI
                     LSVIDKNNSKIIRISYEEPNLEDVFLNLTGKNLRD"
     gene            complement(8920..9567)
                     /locus_tag="L3M60_001609"
     CDS             complement(8920..9567)
                     /locus_tag="L3M60_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226286.1"
                     /translation="MNKIKVLIVDDEKLIRKGLKIILSSYNDLEIVGDASNGYEALEF
                     CKTNDVDIVLMDIRMKVCDGVLGTRLIKEYNNSITLLILTTFNDDEYIKDAMKFGASG
                     YLLKDSSDEVLHEGIRSSFFGNIVLDKSVAEKIMTPEKMIEQEHLYDMYNLTEKEISI
                     IRLIANGLNNKEISKELFLSEGTIKNNITNILGKLELRDRTQLAIFAFKNKIVIE"
     gene            complement(9560..10723)
                     /locus_tag="L3M60_001610"
     CDS             complement(9560..10723)
                     /locus_tag="L3M60_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6226287.1"
                     /translation="MNRKKLVAFIRYLSLFILVVSYLKNVENTLYPVIIMMILFIIII
                     NNQVRFFSLSNENKFILISYFLELILIFILCNYTKTFNSIYFVPLILDISFLIKEKYK
                     YILFTLVIISSLIISLDKNIYLALESSSVLLIITILSIYIENENLSKLYSQNLYDKLR
                     ISKDELKKVNADLETYASSIEELAILKERNRISREIHDSVGHSLSTTIIQLNAIEKLL
                     KDKPLISELVHELREFVTESFQDVRRAISELKPVEYENYQSLFKIKELVKSFIKLTNI
                     NVKLTISKNTWNLSRNQSIGLYRLIQESLSNSSRHGKATEIKIFMTFNPSNFIITISD
                     NGIGCGNIKKGNGLNSISERIYELNGKVEFDNSNNGFTIRASFPKFSGGDFFE"
     gene            10907..11251
                     /locus_tag="L3M60_001611"
     CDS             10907..11251
                     /locus_tag="L3M60_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBN6226288.1"
                     /translation="MNNKEKGDFGEEIAVNYLLSKGARILEKNYRLKIGEIDIIAKMG
                     REVIFVEVKSRSNIKFGYPCESVSYKKRKKIIGIANYYIIKNNLNNTPVRFDVIEVYL
                     LEKRINHIVNAF"
     gene            11282..12883
                     /locus_tag="L3M60_001612"
     CDS             11282..12883
                     /locus_tag="L3M60_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HBN6226289.1"
                     /translation="MLSIINSSNLVGIDSFLVKVEVDISNGIPSFNIVGLPGKEIKEA
                     RERVKSAILNSGYKFPSTRIVVNLSPADIKKEGAFLDLSISIGLLRELIKKDENYIRE
                     SMFIGELSLDGKIRKVRGILPIIMGAKTQKIKRIFIPIENIKESLFVDEIDIIPIKSL
                     KECVDFLNEEIKVDKVRIMSFLNDKSKKENEELEKDNSYIDFKYTKINDEESKYNEDF
                     EDIKGNYFVKRSAEIAAAGNHNMFMIGPPGSGKTMIAKRVRTILPDISIEEMIEVSKV
                     YSILGMINETKGIIDKRPFRAPHHTTTKQSLIGGGMDARPGEIALAHRGILFLDEIAE
                     FDRKILETLRQPIEDGYVNISRVKYSAKYPCRVLLIAAMNPCPCGYYMSETECRCRSN
                     EIDRYINKISGPLLDRFDIFVEVNSIKYSDFNSLKQEESSQKIKRRVENARKIQINRF
                     KKDNIKNNSEIKAYNLFKYCKLEKEASKTAEMIFNKYNLSSRSYTKLLKMARTIADLE
                     ERDLINSQCIIEAFSFRKAYYSYFR"
     gene            12902..14002
                     /gene="dprA"
                     /locus_tag="L3M60_001613"
     CDS             12902..14002
                     /gene="dprA"
                     /locus_tag="L3M60_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBN6226290.1"
                     /translation="MEKRDTYLWLKSIGGITTKTIEIIENEIVNIEDIFDFSEKEIYN
                     LKNISLNIRKNIVKYRGHAYLENIKELLYKKAIKYICQYDKEYPENLKNIYNAPKLLF
                     YKGDIDLVNNKFNIAIVGSRKPTAYGINCAKTISCQLSQYGVNIVSGLAIGIDAYSHI
                     GCMSGKSKTIAVLGSGVDNPLPKQNLHLSNKILENGGLLLSEYNINSTVAPYHFSNRN
                     RIISGLSDGVVVVEAAIKSGALITVDFALEHGKNVFAIPGNINSQMSRGCHKIIKEGA
                     KLIENIDDILNEYNIFNIIDKKINQKYDNISLNAKSKQIIEAIKREGNLHIDSICDYT
                     GIEIKYVNSIINELVLNELVVEMNNKTYSLNV"
     gene            14035..16122
                     /gene="topA"
                     /locus_tag="L3M60_001614"
     CDS             14035..16122
                     /gene="topA"
                     /locus_tag="L3M60_001614"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBN6226291.1"
                     /translation="MAKTLVIVESPAKAKTIEKFLGKSHYTVKASVGHVRDLPKSKLG
                     VDIENNFEPQYINIRGKGDVIKELKKEAKKSKQVYLATDPDREGEAISWHLAHILNLD
                     ESEDCRIEFNEITKDAIKKAIKHPRNIDINLVDAQQARRVLDRLLGYQISPILWQKVR
                     KGLSAGRVQSVTTKLICDREKEIKAFIPKEYWTIDVEAKTVKDKDITLKFYGKNDEKI
                     ELANEEAVNEILKEMEGKDLKVEKIESKSRRKSAPKPFTTSMLQQEGANKLFFTTKKT
                     MMIAQELYEGIDIEGEGTVGLISYIRTDSKRISDEAKEKAKGYIVNNLGENYYKNTGD
                     KKEKKDAKKVQDAHEAIRPTSVDRTPDSIKASLSKDQYKLYNLIWRRFVASQMEDSIF
                     DILNVECKIGDIVFKATGSKMKFDGYTKVYNFSEREDKILPSIEKGDILNIENILPDQ
                     HFTQPPARYTEASLVKTLEELGIGRPSTYAPTIATILNREYVEKQGTSLCPTELGIIV
                     TDILENNFHKFIDVDFTADMESKLDVIEEGNTDWKKIVSESYAPLKEAIEEALENIEK
                     VNMDEETDEICENCGSNMVIKYGRFGKFMACKNYPDCKTTRPIMNKIGVKCPKCEEGD
                     IILRKSKRGKAFYGCSNYPNCDFVAWNKPTGEFCEKCGSYMVEKVTKSETKTVCSNKE
                     CNKETKEHNDGKE"
     gene            16287..17072
                     /gene="codY"
                     /locus_tag="L3M60_001615"
     CDS             16287..17072
                     /gene="codY"
                     /locus_tag="L3M60_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HBN6226292.1"
                     /translation="MASEVLQKTRKINKTLQTSGGSSVSFDLLAGALGDVLSSNVYVV
                     SAKGKVLGLHLNDAQDSSVIEDEYTKQKKFSDEYTQNVLKIDETLENLNGEKILEIFP
                     EEHGRLQKYTTVVPILGSGQRLGTLVLSRYSNSFNDDDLVIAEYSATVVGLEILRAIG
                     EELEEEMRKKAVVQMAIGTLSYSELEAVEHIFAELDGKEGLLVASKIADRVGITRSVI
                     VNALRKFESAGVIESRSLGMKGTHIRILNDKLTDELKKLKNNQ"
     gene            17188..18453
                     /locus_tag="L3M60_001616"
     CDS             17188..18453
                     /locus_tag="L3M60_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HBN6226293.1"
                     /translation="MPLADKIRPKTMNDVIGQSHIIGNGKILSKILQSNFLPNMIFFG
                     PPGVGKTTVAEIIAERSNKNFYKINATNSSLEDIKNVISELGSINNISGVLLYIDEIQ
                     SFNKKQQQSILEFMENGSITLIASTTENPYHYVYKALLSRSTVFEFKPLEKIDIEKGL
                     KRAVEVLNKDSYMDIECNKDAIEYIAILSDGDMRRALNILEVVVYSTKPNKDNITYID
                     SDIIKASTFNKIINYDKNGDSHYDILSAFQKSIRGSDPQASIHYLARLIKGGDLISIC
                     RRLLVIASEDIGLAYPNAIVIVKACVDSAMQLGFPEAKIPLAEATILLATSPKSNSAC
                     IAIMKAMDELDKEFVKDIPNSIKDAHYSGSKEIGRGCGYKYPHNFKNHYVKQQYLPDS
                     IKDSVYYIPQENKTEKNIKKYLEYLDGDF"
     gene            18484..19254
                     /locus_tag="L3M60_001617"
     CDS             18484..19254
                     /locus_tag="L3M60_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6226294.1"
                     /translation="MIIKLNSTYHSRVMKYLKKEPEYNLFIIGDIERYGYGNNFLNIW
                     ADIDELGEIKAILLKYFEFMMFYSDGEYDVEGFYNLLRNTNYEEISGKICAVDALAKR
                     LGLNNLKVVDFCKLQTKKFLIDNNCNAKVKRIRLGNLKKTVKLYDLIDEFHSTTLENL
                     KNGLRTGRGYCIEINKQVVSMAKSTSENRTHAMIIGVGTHPKYRARGLATKCLIKLCS
                     ELLRENKIPCLFYDNEEAGKIYKKLGFENIGKWGIYSK"
     gene            19363..19809
                     /locus_tag="L3M60_001618"
     CDS             19363..19809
                     /locus_tag="L3M60_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBN6226295.1"
                     /translation="MKLSTKGRYGLKAMFELALNQDNGPVSLKFIAKKQKISDQYLEQ
                     IFSSLKKSGLVKSVRGAQGGYLLSKNAEDITVGDILVVLEGPVALSDCVLDEDVCENS
                     NMCVTKIVWEKMKKGIEDVIDSITLKDMINDYNKNKLENDITNIKK"
     gene            19827..21020
                     /gene="nifS"
                     /locus_tag="L3M60_001619"
     CDS             19827..21020
                     /gene="nifS"
                     /locus_tag="L3M60_001619"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HBN6226296.1"
                     /translation="MEKRRLYMDYSATTPIKKEVLDAMMPYLTDYFGNASSFHTFGRE
                     AKDALDKAREQVAALINAEPSEIYFTAGGSESDNWTLEGVAYANKNKGNHIITSKIEH
                     HAILHTCEYLAKHHGFEITYLDVDSEGKVDLKQLEDSIKDTTILISIMFANNEIGTIQ
                     PIKEISEIAKKHKILFHTDAVQATGNIPVDVKELGIDLMSMSSHKIYGPKGVGALYIR
                     KGVRLHNFVHGGAQEKSKRAGTENIPAIVGYGKAAELAKENMQNHVETLTRLRNKLID
                     GVLERIPYTRVNGSLENRLPGNANFAFQFIEGEGILLLLDMLGIAGSSGSACTSGSLD
                     PSHVLLAIGLPHEIAHGSLRLTVGDFTTDDDIDYILENLPKVIERLRSMSPLYDDAKK
                     QGLVK"
     gene            21074..21514
                     /gene="nifU"
                     /locus_tag="L3M60_001620"
     CDS             21074..21514
                     /gene="nifU"
                     /locus_tag="L3M60_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HBN6226297.1"
                     /translation="MQYSDKVMEHFMNPRNMGEIDNASGVGEVGNPTCGDIMKIFLDI
                     DGDVIKDVKFKTFGCGSAIASSSMATEMIKGKTIKDALELTNKAVAEALDGLPPVKMH
                     CSVLAEQAVKAALIDYAKKNNIHIPELDGYVIDDAHDHDVEEEE"
     gene            21798..22877
                     /gene="mnmA"
                     /locus_tag="L3M60_001621"
     CDS             21798..22877
                     /gene="mnmA"
                     /locus_tag="L3M60_001621"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBN6226298.1"
                     /translation="MNKKVMIGMSGGVDSSVAAYLLKQQGYDVIGVTMKLWQDDDDVE
                     IEGGCCSLSAVEDARRVANKIGIPFYVLNFREVFKEKVIDYFIDEYLEGKTPNPCIAC
                     NKHIKFDDFYKKARQIGCDYVATGHYAKIEKDESTGRYLLKKSVTDKKDQTYALYNLT
                     QEQLEHTLLPIGDYEKDRVREIAKEMGMAVHNKPDSQEICFVKDNDYANYVKKHSKKR
                     IEEGFFVDTKGNILGKHKGILYYTIGQRKGLGITFGKPMFVIDINPINNTIVLGDNEN
                     LFKKELIAKDVNFISIDTLEEPLRVQAKIRYSAKPSPATIHRVGKDTIKIVFDEAQRA
                     ITKGQSVVMYDEDIVVGGGIIEKSL"
BASE COUNT         9246 a         2505 c         3857 g         7290 t
ORIGIN      
        1 ataaaacatc tttatattca ataatatata tttttgtaaa gaaaaaataa agaatttatt
       61 gtataaacac ttattaatta atcaaggtta aaataaaaaa atataaaaaa aataaagtat
      121 ataagagtat aattagactt ttatatactt ttttatatta tttttgagtt ttttaggaag
      181 atattttaca agttgagaaa actataagga gtgttatatt gaatataaaa tagtataata
      241 tatattatgt tagataatta atatgaaaat atacaaatat ataaaaagtt tgtttagagt
      301 tatagttaat gtgtgtatta atttaatact aatttattgg aggtctaaaa atgaagtaca
      361 taataaaaag agatggtaca aaagaacttt ttagcaaaag taagattgaa gatgctgtct
      421 ataaagctag tataaatagt gaaggtggag tagatagaga tttggcatat gatatatcaa
      481 gaaaaatagc caatgactat aattctatgg aaaaatgctt atccgtagaa gaaatacaag
      541 atatagttga agtactactt atggaatcaa atagaaaaga tatagcaaaa cactacatat
      601 tatatagaga aagacgctca gaaataagaa ctaaagcttg ggagtttgat gaattacaaa
      661 aatcaatatg gaagaacaag tatagacata atggtgagag ttttgatgaa tggataaaga
      721 gaatttcaaa taatagttca agaatatcaa aactaataag acaaaaaaga tttctatttg
      781 ctggaagaat tttagcaaat agaggattac agaaagaagg aaaaaaagta acatattcca
      841 attgttatgt aatagaacca ccaaaagata gcatagaaga tattttcgat actgcaaaaa
      901 aacttgctag aacttttagt tatggtggag gagttggaat tgacatttca aagctaagac
      961 caaaaggagc gaaagttaac aatgctgcaa aacatacgac tggagcagtc tcttttatgg
     1021 aactttatag tatgacaact ggcttaattg gtcaaagagg gagaagagga gctcttatga
     1081 tttctatgga tgtaaatcat ccagagatag aagagtttat agatataaaa acagatttgg
     1141 agaaggttac taaagcaaat atttctgtaa gagtaacaga tgaatttatg caagctgtaa
     1201 gggaaaaaaa gttatataat tgtacttttg aagttgaaac tactggagaa aaaatagtta
     1261 agatagtaaa tgcgtatgat ttatttaata aatttgtgaa aaataactgg gattatgcag
     1321 aaccaggtat attattctgg gataaaataa aaacatataa tcttatgagt gaagataaga
     1381 acttttgctt ttcaggagta aatccttgtg cagaagagcc acttccaagt ggaggtagtt
     1441 gtctacttgg ctcaataaac ttatcagagt ttataataca accatttggt gaacatgcta
     1501 catttgatat taataaacta aaatcttgcg ttagagaagc tgtagtagct ttaaatgatg
     1561 tcttagatga gggattaaac ttgcacccat tacaagaaca aagagatagt gtacataaat
     1621 atagacagat aggtttaggt gtaatgggta tagctgatat gcttataaaa atgggaataa
     1681 gatatggctc tgatgaatct ttagaattat gtgatttaat tggtaagact atgttaaatg
     1741 aagctgtaaa gcaatcagca ttaattgcta aagaatatgg cacttttagt gagtataaat
     1801 atgaaaatat ttctaaatct aagtttttta tagaaaattt aaatgaagac actaaggaaa
     1861 tggttaaaaa gtatgggctt agaaactcac aacttttaac aatacctcct acaggttcta
     1921 tatctacaat gataggtatt agtggtggaa tagaacctat atttaatctt tcatatataa
     1981 gaaaaactga aagcttacat gatgaagatg tgtactataa agtatatact ccaattgtta
     2041 aagagtatat ggataagaaa ggtattacag atgaagaatg tttaccagat ttttttgtta
     2101 ctgctatgaa tataggatat gaagaaagaa taaacatgca aaaagtatgg caaaagtata
     2161 ttgatgcttc tatatcatct acaataaata taccttttga aactacagtt gaagatgttt
     2221 tcaatatata tattaaggca tgggaggctg gtcttaaagg agtaacaata tttagagatg
     2281 gatgtaaaag gagtggaatt ttaataaatg aaaaaaagga agaaaataat aaagaaaata
     2341 aaaaagaaaa tagtaaaaaa gaatattata aaaataataa aaaagaaaaa gataataaag
     2401 atgaaaaagc caaagaaata ataaataaaa attttgaaga agatgaaaaa tttgtgtgcc
     2461 ctgagtgtgg aagtgaagga attgcacata caggtggatg tagtatatgt ttaaaatgtg
     2521 gttatagtgg atgcaattaa actctaatta agaaatttta atatgtttaa aacttaaatt
     2581 tttaaaaacg aaagatgggg gaatggtatg caggataaaa gtgtgaggga gattaaagaa
     2641 ataatagaga ctttagaagt agaaaaatat atggaatata tagaactatt aagggtagat
     2701 gaaagaaaat ctgtacaagg tttagctata aaactagcaa aaaaactaga taatattaga
     2761 aaagaagaag agagattaga aactataaat atatttgaga acgaaggcta taataaaggc
     2821 tatttatata ttggaggaat tgatgaggcg ggaagggggc cacttgctgg acctgttgta
     2881 gcttctgttg tagtatttaa aaaagataca aaaatagaag gtgtaaatga ctctaagaaa
     2941 ttgagtgagg caaaaagaga tgaacttttt gaagtaataa aagaggaagc actagattat
     3001 ggaataggta tagtaaacaa tgaagaaata gatgaattta atatattaaa tgctacatat
     3061 atggcaatga aaaaggctat aaattgttta aaaaaagcac cagattattt attagttgat
     3121 gcagcaacaa taccaggtat tgatatagct caaaatccaa ttgtaaaagg agattcaaaa
     3181 tctatatcaa tagcagccgc aagtatactt gctaaagtta ctagggatag tataatgtac
     3241 caatatgata gagtataccc agaatatgga tttaaatctc acaaaggata cggaactaaa
     3301 gaacactatg aagctattga aaaatatggc ataactccta tccacagaaa aagttttttg
     3361 aaaaatatac tgtaaatata atatgatata atgtatataa tataaattac tataattaat
     3421 gggggattaa agatggaata taatgaatta ttaaaaaatg ctagagaaaa tttaaatgga
     3481 agttgtaaag tttgtaaggt ttgcaatggg gttgcttgtg caggcgaagt acctggcatg
     3541 ggaggtaaag gaagtggttc ttcttttata gaaaatagca agagtttaga gaaagtaaaa
     3601 gtaaatatga gagtaataca tgatgtatca aatcctgata catctataga gatgtttgga
     3661 aagaaaatga gtgcacctat atttgcagct ccagtaacag gaactacttt aaatatgggt
     3721 ggaaaaatta atgaaagaga ttacatagaa ccagtagttg caggatgtgc taatagtggt
     3781 atttatgcaa tggttggtga tacagcagta gatgcttttt taatggaaaa tttagatgta
     3841 gttaaaaagt ataatggagc tggaattgta tttattaaac cttgggataa tgaaaatatt
     3901 ataaaaaaga taagattagc tgaagaagcg ggtgcatttg cagttggagt tgatatagat
     3961 gcttgcggtc tagtaacttt atcactacat ggaaaaccag tacttcctaa aaatgttgaa
     4021 caaataaaag agttagtaaa gtctactaaa ctaccattta tattgaaagg tataatgact
     4081 gttgaggatg ctttaatggc agtagaagca ggtgtggatg caatagttgt atcaaaccat
     4141 ggaggaagag tacttgattg tacaccagga gcatgtgaag ttttaccaaa aattgcggat
     4201 gctgttaaag gtaaagttac aatactcgca gatggtggag tgagaactgg tttagacgta
     4261 ctaaaaatga taggtttagg tgcagatgca gtattaatag gaagaccttt tgtgactgct
     4321 tcatttggtg gtgcaactga tggtgtagaa acttatgtaa ataaacttca atcagagcta
     4381 agtagctcta tgatattaac tggatgtcaa actataaaag atattgatgg aaaagttata
     4441 tacaaataaa attttataca aaaaagagga aaaatcgttt taatgtagaa taaattgtac
     4501 aaagggggaa tttatatgag tagaaaagct tatgaagaag ccttagtaga attagaaaaa
     4561 tttattgatg aaagaaaaga aataattaaa agtgcggagg actgtattga taagtacata
     4621 gttgacagaa cgctaccttt tgattacaaa gataaatgtg tggaatggca acaagagtta
     4681 cttgatattg cagaggcaca agttttagaa gcaaatgaat taagtgtatt attacaagag
     4741 aaaaaagaac ttgaagaaga ttaagtttta ttatctaaga gtaaaccagt aatatgattt
     4801 actcttagtt tttttatttg aataaagaca tctatctact tagggtaacc ttattaatat
     4861 tagattaggc agtttaaagt aagatagagt agcataaatg attagtataa aaataaaacc
     4921 agtctttaag actggtttaa tttttacttt tagaatttag tatatgtttt ttcatttctt
     4981 ctaatactac tgtataagat ttacattgag cagcagccaa ttctgataaa gcaaatcttt
     5041 gagctttatt taaaaactcc ttatctgtac tacacaattg ttttgaacag tcttgtatca
     5101 taagcatttt aattaatttt acaatctcaa aaacatctcc tgaatgaaat ttatcatggt
     5161 acagtttaag cctttgtttt ctatcaaaaa cccatacacc atctgtttta tcaatagatt
     5221 gaataaattt atcaacgtca tttccatcca taactggacg tattccaatc ctgtctgcat
     5281 ttagaactgg aatcgaaatt ttaaggttac tattgaaaat aacacttaat tcatagtatt
     5341 tttgcatctg atgatttatt ttctttgtta caatatcatt tacacagcag gctccctctt
     5401 ttggatacat cacactttca ccaattttat acatatatta gttatacctc caaactgaat
     5461 tttagatatt gcaacttagg tacagtatat taattatcta ataattaata ttataccata
     5521 aaaaacttga caagtcaaaa atgttaatat ataatattaa gtttaggtta tatggtatat
     5581 aaaactaaat agaaaaatat aaggtaaaat gatttaacat gttggaatat tcttttatca
     5641 ttatataaaa ataaatacta attatattta ataactacta gtttttagca aaatctcgta
     5701 acttaagact tcctgcagtt acaaatatag ttccaatcaa tatcagaatt attatattta
     5761 ttaagatact accattgttt ttaatagcat ctatagtcca ataaaatggt gtaagttttg
     5821 aaagattagc cataaatgat aatttatcca ttgatggtat tagactagat agagatatag
     5881 ccaaacctat taaagaataa aatgtagata taagacttat agaactttca tttttaaata
     5941 ctctagctat ccaaaataca aatccagtgg aaacaaaact atttgctagt acaatcatca
     6001 atacattcag acttaaatta aagtcttcta taattgaaaa acataataga gtaaaagtgt
     6061 atacaattga ttgaataaaa aataacccta aaaatatact gaaaattatt tttatatctt
     6121 tattatctgt agagataagt ctttttagca catttgattt ttttaatgta aataaatcag
     6181 aagcaagatg tgccgcatta atatacataa aatagcatat caagaagact actaaaaatg
     6241 aacctttatt cttatctagt ttgttatcaa ttatatttgt ctttgtcaat ttatcatcta
     6301 tatttttatc cattttcaat tttaacgatt tagtaataaa agattctatt tgtgattctg
     6361 cccaaagact accaccacca ttttcaattt taaagcattt aactacaggt cgttgtaagt
     6421 tatctatact atttgaaaaa tttttatcta agacgaatac agcagaaacc ttattatttt
     6481 ttaatagatt catagcctta tcttgttcat ttaatctaaa tgaattttct tttaacttag
     6541 aactatttat taacttatgt tcatactttc ctgaagagtc agatacaatt gctactggag
     6601 aaaagtacat tgattgattt cccgagttgt tgataaaaaa attaagtgaa aaaattagta
     6661 taataggaag cataaccata ttaattaaaa gagtatgatt ttttagatat ctttttatat
     6721 ttgcaacagt taatctaatc attattgaac ctccctccat ttatattttt cctttacaat
     6781 agcaagtgca atcaatattg tagaagtaat taaacatgtg aaaattaaat ttcctattga
     6841 atctatagtt ttaaatgttt cataattact aaacaattct cctattaaat aggtaggaga
     6901 taaacttgta agcttagtaa atgcatcact tatcatacta aagatacctc caaaaattgt
     6961 ctgtaaaaag agtaatgcat aaactattat tgtaccatat tttttactaa aaaagctgtt
     7021 tacaaaagta gatattgaag tcatgaaata agatgataaa gcacaaagta ggagcaatat
     7081 ggcaaaatta cctttaaatg ttattccaat tattctaaag aataaaacat ataataataa
     7141 gaatataaat gaataaatcc ataaacctac aaagtcataa attaagaatt gtactctagt
     7201 tataggcata gaataaagtc tttttgagag tccttttgat tcgcttatat aatttgataa
     7261 aatattgttc attataaaga ttattacaag aaaccctagc gaaactaaag caaagtattc
     7321 ataagagctc tgtttaacat tgttacctat tatagaagta tcaatagaat ttttattaaa
     7381 taatttagag aaatcttctg aacttatttt ctgggaatta tttaaataaa atttttcatg
     7441 gtaagtatct aatatgtctt gaagcaatat tgcaatatca tcacgagttc caagtttttc
     7501 tatatttaga gtatttgatt tttcatttaa taaactactc tcatagcctt taggtatagt
     7561 aagctttaat tctgccttag aatcatcttt tttaactgtt aatatataag ataaatcatt
     7621 tttcaaatac tttgtcaaat gatttgataa tcttgtgttg tctttgtcat atataacaat
     7681 gggtgtagat tctgttttta taggattttg aaacatgctt tctgtaacca tcccaagaaa
     7741 ccaagataaa atcagaggta aagctaaaaa atatattaaa ttaagtataa aggagctttt
     7801 taaacccttt agcatagttt ttacactcat gtataatttc ataaaattac ctccattagt
     7861 cccttaaatt ttttcctgtt agatttaaaa atacgtcttc tagattaggt tcttcataag
     7921 aaattcttat tatttttgag ttgtttttgt caatgacaga gagtatctct gtaagttgta
     7981 attttttatc tatatccaat tctaataatg aattattttc taaaatagat ataacacctt
     8041 tagtattttt taaatcaaag ataagttgag catttatatt ttttatttcc ataattaatt
     8101 ttgtatttgt agatactagc gattttaaat aagcattatc tccaaaagca atctctttac
     8161 cttcatccat aataaaaatt ttagaacaaa gatgttcaac ttcctccata taatgggaag
     8221 tatatattac agtagtttca tattttttag acatatcttt tataaaattg aaaatatgat
     8281 ttcttgattg ggggtcaaca cctacagttg gttcatccaa tattaatatt ttaggatgat
     8341 ttaagagtgc tattccaata tttaatctcc tttgcatacc atcagataat tttttgactt
     8401 ttttcttttt gacttcagta agacctgtga cttctaatgc ttcattgatt ctttctttaa
     8461 gtaaatttcc ttttaaacca tagaaagctc caaagtactc taagttgtca aaaggagtga
     8521 gagtttctat aattgctaat tcctgtggaa ctaatcctat tatagacttt gcttttatat
     8581 aatcctttga taaatctaaa ccatttattt gaatactacc actatttggt aacataagat
     8641 ttgtaattat atttataagt gtagatttac ctgcaccatt tggaccaata agtccaaata
     8701 tctcaccttt tttaacagaa aagctgatgt tatcgagtgc taatttatca ttaaatcttt
     8761 ttgttatatt atttactctg actatatcca taataattcc tccaaaataa ttttgtataa
     8821 gttaaaaatc tataatacta taataaagaa ttatgaatta taattctatt actataagtc
     8881 actaaaaaaa gtgacttaag tcactttata tttttaaatt tattctatta caattttatt
     8941 tttgaaagca aagattgcta gttgagttct gtcacgaagt tctagtttac ctaaaatatt
     9001 agttatgtta ttctttattg taccttcaga caagaatagt tctttactta tttctttatt
     9061 attaagtcca tttgcaatta gtctaattat ggatatttct ttttctgtta aattgtacat
     9121 gtcataaaga tgctcttgtt caatcatttt ttcaggggtc atgatttttt cagctacact
     9181 tttatcaagg acaatattgc caaaaaatga agaacgaatc ccttcatgca atacttcatc
     9241 agagctatct tttaataggt atcctgaagc tccaaacttc atagcatctt ttatatattc
     9301 atcatcatta aaagttgtta aaattaataa tgttatagaa ttattatatt cttttataag
     9361 ccttgtacct agaactccat cacatacttt catacgtata tccataagaa ctatgtctac
     9421 atcattcgtt ttacaaaatt ctaaagcttc atatccatta gaagcatctc ctacaatttc
     9481 taaatcatta taagatgata atataatttt aagacctttc ctaattaatt tttcatcatc
     9541 tactataaga actttgattt tattcaaaaa aatcacctcc tgaaaattta ggaaaagaag
     9601 ctctaattgt gaaaccatta ttagaattat caaattcaac tttaccattt aattcatata
     9661 ttctttcact gatactatta agtccatttc ctttttttat attgccacat cctataccat
     9721 tatcactaat agtaattata aaatttgaag gattgaatgt cataaaaatt ttgatttcag
     9781 ttgcctttcc atgccttgat gagttggaaa gagattcctg tataagtcta tataaaccta
     9841 tagattgatt tctagataag ttccaagtat tttttgaaat agttaattta acattgatat
     9901 ttgtaagctt aataaagctt ttaactagtt ctttgatttt aaataaactt tggtaatttt
     9961 catattcaac aggctttaac tcagatatag cccttcttac atcttgaaaa ctttcagtta
    10021 caaattctct aagttcatgt actaattcag aaattagagg cttgtctttt aggagctttt
    10081 ctatagcatt taattgtatt attgttgtag aaagagaatg tcctacacta tcatgtattt
    10141 ctcttgatat tctatttctc tcttttaaaa ttgctaattc ttctatagaa cttgcataag
    10201 tttccaaatc agcgttaacc ttttttaatt catccttgga aattctaagc ttatcataaa
    10261 gattttgact gtataatttt gataaattct cattttctat atatatagaa agtatagtta
    10321 taattagtaa tacagaactt gattccagtg ccaaatatat atttttatct aaactaataa
    10381 taagagaaga tattataact aaagtgaaca atatgtactt atatttttcc tttattaaaa
    10441 atgatatatc taatattaaa ggaacaaagt aaatcgaatt aaaagttttt gtataattac
    10501 acagtataaa aattaatata agttctaaaa agtaagaaat aagtataaat ttattctcat
    10561 ttgataaaga aaaaaatcta acttgattat ttattataat tataaataat atcatcatga
    10621 ttatgactgg atacaatgtg ttttcaacat ttttcaaata gcttacaact aatataaata
    10681 aagataaata tcgtataaaa gcaactaatt tttttctgtt cattaaaggc actcctccaa
    10741 aaaaataaac ttttcctatg aaaactagaa taatatcata aaagaataaa gtcaatagaa
    10801 gtatttaata atattgatag aaataaaatc aatcccaaaa attgaatagt aaaattgtat
    10861 actgaccata atttaaaagt gaaaatttaa tgtattggag tgaatcatga ataataaaga
    10921 aaaaggtgat tttggagaag aaatagcagt taattatttg ctatcaaaag gagcaaggat
    10981 actagaaaaa aattacagat taaagatagg ggagatagat ataatagcaa aaatgggaag
    11041 agaagttata tttgtggagg tgaaatcaag gtctaacata aagtttggct acccttgtga
    11101 atctgtaagt tacaaaaaaa gaaaaaaaat aattggaatt gcaaattatt atattataaa
    11161 aaataactta aataatactc ccgtaagatt tgatgttata gaggtatact tattagaaaa
    11221 aagaataaat cacatagtga atgcttttta aaaaagataa tgtttttgga aaggattaaa
    11281 tatgttaagt attattaatt caagtaactt agtaggaatc gacagctttt tagttaaggt
    11341 tgaagtagat ataagcaatg gaatccctag ttttaatatt gttggtcttc caggtaaaga
    11401 aattaaagaa gcaagagaaa gagtaaagtc agcaattctt aatagtggat ataaatttcc
    11461 aagcacgaga atagttgtaa atttatcacc agcagatata aaaaaagaag gagctttttt
    11521 agatttatct ataagtatag gtcttttaag agagttaata aaaaaagatg aaaattacat
    11581 aagagaaagt atgtttatag gcgaattatc attagatgga aaaataagaa aagtaagagg
    11641 tatattgcct ataattatgg gtgcaaaaac tcagaaaata aagcgtattt ttataccaat
    11701 tgagaacata aaagaaagtc tatttgtaga tgaaattgac ataattccta ttaagagttt
    11761 aaaagaatgt gtcgattttt taaacgaaga aataaaagtc gataaagtaa ggataatgtc
    11821 ttttttgaat gataagtcaa aaaaagagaa tgaagaatta gaaaaagata atagttatat
    11881 agacttcaaa tatactaaga ttaatgatga agaaagtaaa tataatgaag actttgaaga
    11941 tataaaagga aattactttg taaagaggag tgctgaaatt gcagcagctg gaaatcataa
    12001 tatgtttatg attggacctc caggttcagg aaaaactatg attgcaaaaa gagtaaggac
    12061 aattttacca gatataagta tagaagaaat gatagaagta agtaaggttt atagtatatt
    12121 gggtatgata aatgaaacta aaggaataat tgataaaaga ccttttagag cacctcatca
    12181 tacaactaca aagcaatctt taattggagg aggaatggat gcaagacctg gggaaattgc
    12241 tttagcacat agaggtatct tgtttttaga tgagatagct gaatttgata gaaaaatact
    12301 tgagactttg agacaaccta tagaagatgg atatgtaaat atatctagag taaagtacag
    12361 tgcaaaatat ccttgtagag tattattaat tgcagctatg aatccatgtc catgtggtta
    12421 ttatatgtca gaaacagagt gcagatgtag aagtaacgaa attgatagat acataaataa
    12481 aatatcaggt ccattgttag ataggtttga tatttttgta gaagttaatt ctataaaata
    12541 ttcagatttt aattctttaa aacaagaaga aagttctcaa aaaataaaga gaagagtaga
    12601 aaatgctaga aaaatacaga taaacagatt taagaaagat aatataaaaa ataatagtga
    12661 aattaaagct tacaatttat tcaaatattg taaactggaa aaagaagctt cgaaaacggc
    12721 tgagatgata tttaataaat acaacctaag cagtagaagt tatacaaaac ttttaaaaat
    12781 ggcaaggaca atagcagatt tagaagaaag agacttgata aattcacagt gtattataga
    12841 agctttttca tttagaaagg cttattatag ctattttaga tagtgttttg aggtggattt
    12901 tatggaaaaa agagatactt atttatggtt aaaatcaatt ggtggaataa caacaaaaac
    12961 aattgaaata atagaaaatg aaatagtaaa tattgaagat atatttgatt tttctgaaaa
    13021 agaaatatat aatttaaaaa atataagctt gaatattaga aaaaatatag taaaatatag
    13081 aggtcacgct tatttagaaa atataaaaga actactttac aaaaaagcga ttaaatatat
    13141 ttgtcaatat gataaagaat atcctgaaaa tttaaagaat atatacaacg ctccaaagtt
    13201 attattctat aaaggtgaca tagatttagt taataataaa tttaatatag ctatagtagg
    13261 ttcaagaaaa cctacagcct atggaattaa ttgtgcaaaa acaataagtt gtcaactatc
    13321 ccaatatgga gttaatatag taagtggatt agctatagga atagatgcat actcgcacat
    13381 aggttgtatg agtggaaaat ctaaaactat agcagtactt ggttcaggag tagacaatcc
    13441 tcttccaaaa caaaatttac atttatcaaa taaaatatta gaaaatgggg gattattatt
    13501 atcagagtat aatattaact cgacagtagc cccatatcat ttttctaatc gaaataggat
    13561 tatcagtggc ttaagtgatg gagtagttgt tgtagaagca gcaataaaaa gtggtgcatt
    13621 aatcacagta gattttgctc ttgaacatgg aaaaaatgtt tttgcaatac ctggcaatat
    13681 aaattctcaa atgagtagag gttgtcataa aattataaaa gaaggggcaa aattaattga
    13741 aaatatagat gatattttaa atgaatataa catatttaat attattgata aaaaaataaa
    13801 tcaaaaatat gataatataa gtctaaatgc caagtctaag caaataattg aagctataaa
    13861 aagagaagga aacttgcata tagatagtat ttgtgattat actggtatag aaataaagta
    13921 tgtaaattca ataatcaatg aattagtact aaatgagtta gtagtagaaa tgaataataa
    13981 aacgtacagt ttaaatgtat aaattaagat taggtagtgg gggtgtatgg ttttatggcc
    14041 aaaacattag tcatcgtaga atcgcctgca aaagctaaaa ctatagaaaa atttttaggg
    14101 aaaagtcatt acacagttaa agcatcagtt ggtcatgtca gagatttacc taaaagtaaa
    14161 ttaggtgtag atatagaaaa taattttgaa cctcaatata taaatataag aggaaaaggc
    14221 gatgtaataa aggaattaaa aaaagaagct aaaaaatcta aacaagtgta tctagcaact
    14281 gaccctgata gagagggtga agctatatca tggcatttag ctcatatatt aaatttagat
    14341 gaatctgagg attgtagaat agagtttaat gaaataacta aggatgcaat taaaaaagca
    14401 attaaacatc ctagaaatat tgatataaat ttggttgatg cacaacaggc aagaagagta
    14461 ctagacagac ttttaggtta tcaaataagt cctatattat ggcaaaaggt tagaaaaggt
    14521 cttagtgcag gtagagtgca atctgttaca acaaagttaa tttgtgacag agaaaaagaa
    14581 ataaaagcat ttattccaaa agaatattgg acaatagatg ttgaggctaa gacagtaaaa
    14641 gacaaagata taactttaaa attttatggt aaaaatgatg aaaaaattga attagcaaat
    14701 gaagaagctg tgaatgaaat actaaaagaa atggaaggta aagatttaaa ggttgagaag
    14761 atagaatcta aatcaagacg taaatcagct ccaaaaccat ttacaacaag tatgcttcaa
    14821 caagaaggag cgaataagtt attttttact actaaaaaaa caatgatgat agcacaagaa
    14881 ttatatgaag gtatcgacat agaaggagaa ggtactgttg gtcttatatc atatataaga
    14941 acagactcta aaagaatatc tgatgaagca aaagaaaaag ctaagggtta tatagtaaac
    15001 aatctaggag aaaactacta taaaaacact ggtgataaaa aagaaaaaaa ggatgctaaa
    15061 aaggttcaag atgcccatga ggcaataaga ccaacttcag ttgatagaac tcccgatagt
    15121 ataaaggcct cattgagcaa agaccagtac aagttataca atttaatttg gagaagattt
    15181 gtagctagtc aaatggaaga ttcaatattt gatatactaa atgtagaatg taaaatagga
    15241 gatattgtat ttaaagcaac tggttcaaaa atgaaatttg atggttatac taaggtatat
    15301 aatttttcag aaagagaaga caaaatttta ccaagtatag aaaaaggaga tatattaaat
    15361 atagaaaata tattgccaga ccaacatttt acacaaccac cagcaagata tacagaagct
    15421 agtttagtaa aaacgctaga agaactaggt ataggtagac caagtacgta tgctccaaca
    15481 atagctacta tactgaatag agaatatgtt gaaaagcaag gaacaagtct atgtccaacg
    15541 gaacttggaa taatagttac tgatattttg gaaaataatt tccataaatt tatagatgta
    15601 gattttactg ctgatatgga aagtaaactt gatgttatag aagaaggtaa tacagattgg
    15661 aaaaagattg tatctgaatc atatgcacct cttaaagagg ctattgaaga agctcttgaa
    15721 aatatagaaa aggtaaatat ggatgaagaa actgacgaga tttgtgagaa ctgtggttct
    15781 aatatggtta tcaaatatgg tagatttgga aagtttatgg cttgtaaaaa ctatcctgac
    15841 tgtaaaacaa ctagaccaat tatgaataaa attggtgtta aatgtccaaa atgtgaagaa
    15901 ggagatatta tacttagaaa atctaaaaga ggtaaagctt tttatgggtg ttctaattat
    15961 ccaaactgtg actttgtagc ttggaacaag ccaacaggag agttttgtga gaagtgtggt
    16021 tcatatatgg ttgaaaaagt gactaaatct gaaacaaaaa cagtgtgttc taataaagaa
    16081 tgtaataaag aaacaaaaga gcataatgac ggaaaagagt gaaaattgaa aaaaatcaca
    16141 taattctctt gaaaaaggtt ccaatctatg ataatctttc aagtgtaaac aactaataga
    16201 aaaataaatt ttcattaaat gtgaaaataa tattagaaaa tataaaaggt tattaaaagg
    16261 cctaaatttc atgaggagat gattaaatgg caagtgaagt gttacaaaaa acaaggaaaa
    16321 taaataaaac attacaaaca agtggtggaa gcagtgtctc ttttgattta ctggccggag
    16381 cattgggcga cgttttaagt tctaatgttt atgtagtaag tgcaaaaggt aaagtactag
    16441 gtcttcattt aaatgatgct caagacagtt cagttataga agatgagtat actaagcaaa
    16501 agaaattttc agatgaatat actcaaaatg tgttaaaaat cgatgaaaca ttagaaaatt
    16561 taaatggtga gaagatatta gaaatctttc ctgaagaaca tggaagatta caaaaatata
    16621 ctacagtagt tccaatatta ggaagcggtc aaagactagg aacattagta ctttcaagat
    16681 attcaaattc atttaatgat gatgatttag taatagctga atacagtgca actgttgttg
    16741 gtcttgaaat attaagagca ataggtgaag aattagaaga agaaatgaga aagaaagctg
    16801 tagttcaaat ggcaataggc actctgtcct actccgagct tgaagcagtc gaacatattt
    16861 ttgctgaatt ggatggcaaa gaaggtttac ttgtagcaag taagatagct gatagagttg
    16921 gtataactag gtctgtaata gtaaatgcac ttagaaaatt tgagagtgca ggtgtgatag
    16981 aatcaagatc attaggtatg aaaggtactc atataagaat acttaatgac aaactcacag
    17041 atgaattaaa aaaattaaaa aacaatcaat aatctatatt ttataggttt agattagaaa
    17101 aggtatctat ttcaaataga tacctttttt agacgaatta ttgaggtaag gacaaagtaa
    17161 gtatttttat taaaaaggag tgagaaaatg cctttagcag ataaaataag accaaaaaca
    17221 atgaatgatg taataggtca aagtcatata ataggtaatg gaaaaatatt gtctaagata
    17281 ttacaatcta attttttacc aaatatgata ttttttggtc ctcctggcgt tgggaagact
    17341 acagtagcgg aaataatagc agaaagaagt aataaaaatt tctataaaat aaatgctacc
    17401 aattcatctt tagaagatat aaaaaatgtg atatcagaac ttggaagtat aaataatata
    17461 agtggagtct tgttatatat agatgaaata caaagtttta ataaaaaaca gcaacaatca
    17521 atattagaat ttatggaaaa tgggagtata accttgattg ctagcacaac agaaaatcca
    17581 tatcattatg tatacaaagc tcttttaagc aggtctactg tatttgaatt taaaccttta
    17641 gaaaaaattg atatagaaaa agggctgaaa agagctgttg aagtattaaa taaagatagt
    17701 tatatggata ttgagtgtaa taaggatgct attgagtata tagccattct ttctgatgga
    17761 gatatgagaa gagctttaaa tattttagaa gtggtggttt attcaactaa accaaataaa
    17821 gataatataa cttatataga ttcagatata ataaaggcat ctacatttaa taagattata
    17881 aactatgaca aaaatggtga tagtcattat gacattttaa gtgcatttca aaaatccata
    17941 agaggaagtg acccacaggc atctatacac tatctagcaa gacttataaa aggtggagat
    18001 ttgataagta tttgtagaag gcttttagta atagctagtg aagatatagg tttagcttat
    18061 ccaaacgcta ttgtaattgt aaaggcatgt gtagactcag ctatgcaact tggatttcca
    18121 gaagcgaaaa taccattagc agaagctact attcttttgg ctacatctcc aaaatctaat
    18181 tctgcatgta tagcaataat gaaagctatg gatgaactgg ataaagagtt tgttaaggat
    18241 ataccaaata gtataaaaga tgctcattat tctggttcta aagaaatagg taggggttgt
    18301 ggatacaaat atcctcataa ttttaaaaat cattatgtaa aacaacaata tttaccagat
    18361 agcataaaag atagtgtcta ttacatacca caagagaata aaactgagaa aaatataaaa
    18421 aaatatttag agtatctaga tggagatttt tagactgaac tttatgtatc gggggtagat
    18481 attatgatta taaaacttaa ctcaacttat cacagtagag ttatgaaata cttaaaaaaa
    18541 gaacctgagt acaatttgtt tataataggg gatattgaaa gatatggata tggaaataat
    18601 tttttgaata tatgggcaga tatagatgaa cttggtgaaa taaaggcaat attacttaaa
    18661 tattttgaat ttatgatgtt ttattctgat ggagagtatg atgttgaagg tttttacaat
    18721 ctacttcgta atactaatta tgaagaaata tctggtaaaa tatgtgctgt agatgcttta
    18781 gcaaagagat taggacttaa taatttaaag gtagttgatt tttgcaaact tcaaactaaa
    18841 aagtttttga tagataataa ttgtaatgca aaggttaaga gaattaggct tggaaatcta
    18901 aaaaagacag tgaaacttta tgatttaata gatgagtttc acagtacaac tttagagaat
    18961 ttaaaaaatg gactaaggac aggtagagga tattgtatag agattaacaa acaagtagtg
    19021 tcaatggcaa agagtacttc agaaaatagg actcatgcaa tgataattgg agtagggaca
    19081 catcctaaat atagagctag agggcttgcc acaaagtgcc taattaaact atgttcagag
    19141 ttattgagag aaaacaaaat tccttgcctg ttttatgata atgaagaggc tggaaagata
    19201 tataaaaaac taggatttga aaacattgga aaatggggaa tttattcaaa ataaaaaatt
    19261 aaaattaatc ttgacaaaat tagtagggta taataaaatt atcttgaaag taattcctac
    19321 taaaacagta tgaattagat ttctatatag aggtgataat atatgaagtt gtctactaaa
    19381 ggtaggtatg gactaaaagc aatgtttgaa ttagcattaa atcaagacaa tggaccagtg
    19441 tcattaaaat ttattgctaa gaagcaaaaa atatcagatc agtacttaga acagatattt
    19501 tcatctttaa aaaaatcggg tctagtaaaa agtgtaagag gcgctcaagg aggttatttg
    19561 ctttcaaaga atgctgaaga cataacagta ggagatatac ttgtagtatt agaagggcca
    19621 gttgcattat ctgactgtgt actagatgaa gatgtatgtg aaaactcaaa catgtgtgta
    19681 acaaaaatag tttgggaaaa gatgaaaaag ggtatagaag atgttataga ttcaattact
    19741 cttaaagata tgataaatga ctataacaaa aataaattag aaaatgatat aacaaatatt
    19801 aaaaaataga tttggagatg aatgtaatgg aaaaaagaag attgtatatg gattattctg
    19861 caacaacacc tattaaaaaa gaagtattgg atgcaatgat gccataccta acagattatt
    19921 ttggtaatgc atctagtttt cacacattcg gaagagaagc aaaagatgct ttggataaag
    19981 caagagaaca agtagcagcc cttataaatg ctgaacctag tgaaatatat tttacagcag
    20041 gtggttcaga aagtgataac tggacactag agggtgtagc ttatgctaat aaaaacaaag
    20101 gtaatcatat aataacttca aaaatagagc accatgcaat acttcataca tgtgaatact
    20161 tagcaaaaca tcatggattt gaaataactt atttagatgt tgatagcgaa ggaaaagtag
    20221 acttgaaaca actagaagat tcaataaaag atactacgat acttataagc ataatgtttg
    20281 caaataatga aataggaact atacagccta ttaaagaaat atccgaaata gctaaaaaac
    20341 ataagatatt attccacact gatgcagtac aagctacagg taatattcca gtagatgtta
    20401 aagaattagg aatagattta atgagtatgt cttcacataa aatatatgga ccaaaaggtg
    20461 taggagcttt atacataaga aaaggtgtaa gacttcataa ctttgtccat ggtggagcac
    20521 aagaaaaaag caaaagagct ggtacagaaa atatacctgc aatagttgga tatggaaaag
    20581 cagcagaatt agctaaggaa aatatgcaaa atcatgtaga aactttaact cgtcttagaa
    20641 ataagttaat agatggtgtg ttagaaagaa taccttatac aagagtaaat ggtagcttag
    20701 aaaatagatt gccaggaaat gcaaactttg cattccaatt tatagaaggt gaaggaatac
    20761 ttttattatt agatatgtta ggaattgctg gttcaagtgg ttctgcatgt acttctggtt
    20821 cattagaccc ttcacatgta cttcttgcaa taggtttacc acatgaaata gcacatggtt
    20881 cattaagact tacagttgga gattttacaa cagatgatga tatagattat atactagaga
    20941 acttaccaaa agtaatagaa agacttagaa gcatgtcacc actttatgat gatgctaaga
    21001 aacaaggttt agtaaaatag acagaaagat aaagcataaa aaatagacat aaattgagta
    21061 ggaggaaaag ataatgcaat atagtgataa agttatggaa cattttatga atccaagaaa
    21121 tatgggagaa attgataatg caagtggtgt aggtgaagtt ggaaacccta catgtggaga
    21181 tataatgaag atatttttag acatagatgg agatgtaata aaggatgtta agtttaaaac
    21241 atttggctgt ggttcagcta tagcaagttc ttctatggca actgaaatga taaaaggaaa
    21301 aactataaaa gatgcattag aacttacaaa caaagctgtt gcagaagctt tggatggttt
    21361 accaccagta aagatgcatt gttcagtttt agcagaacaa gctgtaaaag cagctcttat
    21421 agactatgct aaaaagaaca atatccatat accagaattg gatgggtatg ttatagatga
    21481 tgctcatgac catgatgttg aagaagaaga ataaaaataa gtaattgtgt tatgagaagt
    21541 ttaaaatgac taaaaagaag tattaatgag atataaaaaa tagctgtttg taaaatagct
    21601 attttttatt gcttaaatta atagttttta cttgttttag aagtcaatta attaacaaaa
    21661 tatatttcta taatatttag ataatgaaaa taattatttt ttttataaaa tagagcaagc
    21721 tatattttaa ataaatacaa tgtttgttct atatgatata ataataagta atgcaaaaga
    21781 tgaggtgaaa tttgtttatg aataaaaaag taatgatagg tatgagtgga ggagttgata
    21841 gctctgtagc tgcatatctt ttaaaacaac aaggatatga tgtcattggt gttactatga
    21901 agttgtggca agatgatgac gatgttgaaa ttgaaggtgg ttgttgctct ttatctgctg
    21961 ttgaagatgc tagaagggta gcaaataaga taggtatacc attttatgtt ttaaatttta
    22021 gagaagtctt taaagaaaaa gtaatagatt attttataga tgaatatttg gaaggaaaaa
    22081 ctcctaatcc ttgtatagct tgtaataaac atataaaatt tgatgatttt tataagaagg
    22141 ctagacaaat aggttgtgat tatgtagcta caggtcacta tgcaaagata gaaaaggatg
    22201 aaagtacagg aagatatttg ttaaaaaaat ctgttacaga taaaaaagac caaacttatg
    22261 cactttataa ccttacacaa gagcaactag agcatacttt attgccaata ggtgactatg
    22321 aaaaagatag agtaagagaa attgctaaag aaatgggtat ggcagtacac aataagcctg
    22381 atagtcaaga aatttgtttt gtcaaagata atgactatgc aaattatgta aaaaaacatt
    22441 ctaaaaagcg tattgaagaa ggtttttttg tagatactaa aggcaatata cttggaaagc
    22501 ataaggggat attatattac actataggtc aaagaaaagg gcttggaatt acttttggaa
    22561 aaccaatgtt tgtaatagac ataaatccaa taaacaatac catagttctt ggagataatg
    22621 aaaatttatt taaaaaggaa cttattgcca aagatgttaa ctttatatca atagatacgt
    22681 tagaagagcc attaagagtc caagccaaaa taagatattc agcaaagcca tcaccagcta
    22741 ctattcatag agtaggaaaa gataccatta aaatagtttt tgatgaagct caaagagcta
    22801 ttactaaagg tcaatctgta gtaatgtatg atgaagatat tgtagtaggt ggaggtataa
    22861 tcgagaaaag tttataaaaa catctaaaaa attaaaaa
//
LOCUS       DAFNNB010000048        22628 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0048, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000048 DAFNNB010000000
VERSION     DAFNNB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0048"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..631
                     /locus_tag="L3M60_001622"
     CDS             <1..631
                     /locus_tag="L3M60_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBN6226299.1"
                     /translation="YVKEFYKTFDEAKGEYFDGFIITGAPVEQMEFEEVDYWNELEEI
                     MDYSKSKTKSTLYICWAAQASLYKYYNVKKLPLSQKCFGVFKHKVDKNSKIVDGFENE
                     FFAPHSRHTTVNIEALKENKELSIVSHSKEAGPYIITNSRDVFVMGHSEYDKYTLDKE
                     YKRDINRGDKISIPQNYYINDDPSEEPTVKWKKHSELLFRNWIKNYLIQ"
     gene            925..1368
                     /locus_tag="L3M60_001623"
     CDS             925..1368
                     /locus_tag="L3M60_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6226300.1"
                     /translation="MDEMSFKKELLELTRDINMKFTTLLTKFYQPLGITAVQALILSE
                     LCEHGEKKISDLGKNLNMTNSNVSVICQRLEKNGFLNRIRDIEDQRIVKVKVTNKSLD
                     IQENISSSIFDNYFENMTSEKLQDMEDIIEGLEKLNKLLTYIDCK"
     gene            1398..3215
                     /gene="ftsH"
                     /locus_tag="L3M60_001624"
     CDS             1398..3215
                     /gene="ftsH"
                     /locus_tag="L3M60_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBN6226301.1"
                     /translation="MDEHKPQKRSALYYYTVIIIVVMFLNALIFPSLMDKKMVEIDYG
                     KFLTMLDTGKVKSVEIENNRIAINPSSSKDKNIYITGRMDDPELVDRLKNAKVEFTKV
                     IPKENSPLLNILLTWIIPIGILMLFGNLMMKSMQKRMGGNAMQFGKSNAKMYVSAQSG
                     KRFNDVAGQDEAKEALSEIVDFLHNPEKYKKIGAQMPKGALLVGPPGTGKTLLAKAVA
                     GEANVPFFSISGSEFVEMFVGMGASRIRDLFKQAKEKAPCIVFIDEIDTIGKKRDNGS
                     GLGGNDEREQTLNQLLSEMDGFDGGIGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLGGREAILKVHAQNVNVSSDIDYNAIARSTSGASGAELANIVNEAALLAVKSGRDI
                     VEQQDFDESVETVIAGYQRKGAVISDKEKKIIAYHEIGHALVAAMQKHSAPVHKITII
                     PRTSGALGYTMQVAEDESVLMSKEEAIDKITTFTGGRAAEEVIFNTCTSGASNDIEQA
                     TKIARAMVTRLGMSEEFDMVALETVSNQYLGGDTSLACSSDTAAKIDEEVFKIVKTCH
                     KNAIEILENNIDKLHELAKYLLEKETITGDEFMEILNLN"
     gene            3393..6095
                     /locus_tag="L3M60_001625"
     CDS             3393..6095
                     /locus_tag="L3M60_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HBN6226302.1"
                     /translation="MDRPNPDILLEKIKNEEEKLSRGQLKIFFGYASGVGKTYSMLES
                     AQNLKKVGVDVVVGYIEPHTRPETLALLDGLEILPVKEIEYKSIKLKEFDLDAALARK
                     PEVILVDEFAHSNVAGLRHTKRWQDIEELLLAGINVYTTVNVQHLESLNDIVEIITNV
                     SVKETIPDKFLDTNTQLELIDVEPDVLLERFNEGKIYKKEQATRAKNNFFIKDNLVAL
                     REIALRKTAERVNKEVQMTRLSKGDVTVIPTSDTLLACISPSPSSAKVIRTASRISDS
                     TFAKWIALYVETPNTARLPFDEQKQLQNNLKLAKKLGGEIIVLHGENIIEQILRIAKL
                     RNVTKIVIGRNHSSNKRFSKKFKKDIVDKLIDEVDYIDIHIIPYKFASDVKYKPKKDK
                     VSIKSKFKISKVDFIKLIFITLMISILAYAVQSMGFIRENILLIYMLGVVLVSMWTKG
                     YSTGIISSVFNIVLLNYFFTAPLYTLSIADSNYIVTLVVFSIVGIITSTLTSKIQHEA
                     ETASKREENTKMIYQIIKGFLRLSNKDDIINKGIELLSLSLSRDVICYLNVDKYNKSK
                     LYKKNTNNKDKNDLDSEDEKAVASWVLSNSTVAGNDTDTLPGSKGYYIPIVGMNLTLG
                     VVGISCVDSKLDTEDISLIETIIAQMAIALDREILSEAKENTNLEIERERLRSNLLRA
                     VSHDLRTPLAGISGAVSTIIKNKGTIGQDIIDELLSGVYEDTQWLIRLVENLLSMTKI
                     DEGKLEVKKHSELVEEIISEALQKIKKRIENALIDINIPEQILFVPMDAKLIEQVLIN
                     LIDNSLKYSKEDCKIEINVYEKDDYVWFEVSDNGPGISKELKKHIFDRFFTGEEGAKD
                     SRKGVGLGLSICKSIIQAHKGEIMVEDNKDKGSTFKFSLPKENE"
     gene            6129..6827
                     /locus_tag="L3M60_001626"
     CDS             6129..6827
                     /locus_tag="L3M60_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226303.1"
                     /translation="MSKKLILLVEDDKTIRKFISTALLTQDYDVKEAITGKEGISIAV
                     SYSPDVVLLDLGLEDMDGIEVIKAIRQFSNIPIIVVSAREQDRDKVEVFDAGADDYLT
                     KPFSIVELLARVRVAFRHSQVEVQQKDDVKSTFEVDKLLIDFDKRKVIVDDIEVHLTP
                     IEYNILSLLAKHHGKVLTHNFIIKEIWGSVIGNETKSLRVFMATLRRKIEKQPANPRY
                     IITEVGVGYRLNDE"
     gene            7184..8140
                     /locus_tag="L3M60_001627"
     CDS             7184..8140
                     /locus_tag="L3M60_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HBN6226304.1"
                     /translation="MYILNLNTRETIEDFRDKFYVAENSYLILSAPKNLKLLKETLDI
                     DEITFNDCLKFDEITKLDLFDNYDFLSLNTFELRDGEAVIEEVNMYLSDNFILVVVNE
                     EHFLFEFVKNIILKNSQLEKNPVINLFKINYLILREVIKNGFESLEKVEELILQIEDE
                     MMDNINKNHVSRISDVRGLTRIIVKNTRPLLYIGDRIVKENIRYLKYSNVKKYNLENF
                     QGIDFGIDKLYSFALSTRELADKLLDIYSSRVGEKTNNLITKLTLLTAISAPLTIITG
                     IYGMNFRYMPELNWIYGYPATLFFMLCIIFVGIIIFKIKKLL"
     gene            8602..9615
                     /gene="aroF"
                     /locus_tag="L3M60_001628"
     CDS             8602..9615
                     /gene="aroF"
                     /locus_tag="L3M60_001628"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HBN6226305.1"
                     /translation="MIVVLKIGADKNEVKKLIEAIGREGVEVNPIDGTELTVLGLVGD
                     TSKIDAKRIEANKIVEKVMHVVEPFKKANRKFHPEPSIINVNGMEIGSKKIAMIAGPC
                     SVETEEQIVSIAEDVKKSGAGFLRGGAFKPRTSPYAFQGLRYDGLDLLKKAKEKTGLP
                     IVTEIMSTQDIDIFEENVDVIQVGARNMQNFDLLKELGKTNKTILLKRGLSATIEEWL
                     MSAEYIMAGGNENVVLCERGIRTFETYTRNTLDLSAILAVKKLSHLPVIVDPSHAAGK
                     SWMVDSLSKAAIAVGADGLIIEVHNDPAHALCDGKQSIKPNEYDELISELKTIASAVG
                     REI"
     gene            9637..10701
                     /locus_tag="L3M60_001629"
     CDS             9637..10701
                     /locus_tag="L3M60_001629"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBN6226306.1"
                     /translation="MEEKLLVNLKENSYNIFIKKNILNNVGEEIKRIYFGNKIFIVTD
                     ENVAKLYLDKLKNNLSESGFEIKSLILKSGEETKSFNTLPKIYDSLLDFKLTRKDLII
                     TLGGGVIGDLGGFAAATFLRGINFIQIPTSLLAQVDSSVGGKVGVDLERGKNLVGSFY
                     QPKAVFIDPNVLLSLPEKFYRDGMAEVIKYGCIKDKTFFNKLLSLKNREEVMQHIEYI
                     IHKCCFIKKTVVEADEKDFGDRMLLNFGHTLGHAIEKHYNFTGYTHGEAVAIGMYKIT
                     LESEKIGITKKGTSELIKSILMNYNLPYEIELRDNKSIVEAISLDKKNLGDTLKIILL
                     KDIGESIIYNTTSKFFQEVR"
     gene            10701..12014
                     /gene="aroA"
                     /locus_tag="L3M60_001630"
     CDS             10701..12014
                     /gene="aroA"
                     /locus_tag="L3M60_001630"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBN6226307.1"
                     /translation="MGSLKIYPSKLSGDVKIPPSKSIAHRAVICSSLSNGKSRISNID
                     FSDDIIATIRAMTSLGAIIEKKEDILEISGIFSKEGILNRENQLNQPKLTIDCNESGS
                     TLRFLVPISLAFDGVKRFIGRGNLGKRPLDTYYEIFNRQNIKYSYKENQLDLIISGKL
                     KPDEFRVKGNISSQFITGLLFILPTLESDSKIIITTELESKGYLDLTLSTMKDFGIEI
                     INNNYKEFIIKGSQTYKARDYKVEGDYSQGAFYLSADAIGEDINILDLKEDSLQGDSE
                     VVEILSRMGMEILREGNKIKGITNGLNSTLIDASQCPDIIPVLSVVAALSRGKTTIIN
                     AGRLRIKECDRLHAINIELSKLGANIEEKEDSLIIEGVSKLNGGVEVWSHKDHRIAMT
                     LAIASCRCDKPIILKDFECVSKSYPHFFKDFEMLGGRIDEWNMGK"
     gene            11992..13071
                     /gene="aroC"
                     /locus_tag="L3M60_001631"
     CDS             11992..13071
                     /gene="aroC"
                     /locus_tag="L3M60_001631"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBN6226308.1"
                     /translation="MSGIWGNNLKISIFGESHGNAIGINIDGLPSGVELDLEKIDREM
                     KRRAPGKNSISTSRNESDIPEILSGYFNGRTTGTPLCAIIRNSDTRSKDYGELKNLMR
                     PGHADFTGNVRYSGFNDYRGGGHFSGRITAPLVFCGAICKQILSQKGIEVGAHIKKIK
                     NIEDMSFNYVNISKQQLSNLQTLELPLLDLSKEEKMKNTIIDAKNQRDSVGGIIECAV
                     VGVNVGFGNPFFDSVESTLSHLLFSVPAVKGVEFGLGFELADMYGSQSNDEMYYEGNQ
                     VKSKTNNNGGIIGGITTGMPIIFKVAIKPTPSISRQQNTVNIKEKKDDILSIKGRHDP
                     CIVQRVIPVIEAVTAIGIFDLMKGR"
     gene            13074..14270
                     /gene="pheA"
                     /locus_tag="L3M60_001632"
     CDS             13074..14270
                     /gene="pheA"
                     /locus_tag="L3M60_001632"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HBN6226309.1"
                     /translation="MEDLTRYRNEIDNIDKELIQLFEKRMNTVLEIARHKMKNNTTIL
                     QKDREEKVLSRAVDNLDNKAYSQETIQFFNSIMEISRGLQKRLMDNDSEQKIEFRLDS
                     SKKKFDLSSVNKYKSLKEELNKKNILVGFPGKSGSFTEEALNKFFNKKTAKKQFKEFE
                     DVFIALKNKKIDYGIIPIENSSTGAISETYDLLRKYGFYIVGEECIKIDQNLIGIKDT
                     KLKDIKEIYSHPQGIGQCSEFLKQNSAWKLIPFNNTATSAELVKKLQDKTKAAIASKK
                     AASIYGLEIISPCINDITNNYTRFVVISNQIHIEEESNKMSVVFSVEHKAGKLYKVLG
                     YFAENNINMTKIESRPMKNTSWRYFFYIDFECSIYNSKVYNLLELIELNTAYFKFMGV
                     YKNKTK"
     gene            14310..15119
                     /gene="aroE"
                     /locus_tag="L3M60_001633"
     CDS             14310..15119
                     /gene="aroE"
                     /locus_tag="L3M60_001633"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBN6226310.1"
                     /translation="MNFFGLVGEKLSHSVSPQIHKRVFEILNIESAYKNFEISKEDVS
                     KLASAIKLLGIQGVNVTVPYKERIMKYLDFISPEAKRIGAVNTILLREDMLYGYNTDY
                     FGLDSMFKMANIDVQGKVAVILGTGGASKAAITYLLDSGIEKVYVSTRKKDDKKLLNS
                     KAILVDYEELKCITGDIILNATPVGMYPNVGISPVSKSIIQNFDTLVDLIYNPGETEF
                     LRIGNSIGKKTCDGLYMLVGQAIKSQEIWQNAKIDNSILDVIYNELKLEFL"
     gene            15131..15664
                     /locus_tag="L3M60_001634"
     CDS             15131..15664
                     /locus_tag="L3M60_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HBN6226311.1"
                     /translation="MTNKTKEKLILIGMPGSGKTTIGKLLAREYNCSFCDMDDYIVKI
                     AQKSIAELFSEGEDIFRNYETQACRELSISNKAVISTGGGVIKKDVNMEILKETGIII
                     FIDRPIQKILEDININSRPLLKNGKDRLYNLYNERINLYKKFSDIEILNDKSLNTAVY
                     NIINAVSEKFKFEFKEK"
     gene            15685..16524
                     /locus_tag="L3M60_001635"
     CDS             15685..16524
                     /locus_tag="L3M60_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HBN6226312.1"
                     /translation="MNIVIVGLGVIGGSFAKALKKAGYENVFGVDVDLETLKKAEKAK
                     IIKKGCTTGKELFKKADLIILSIYPRLVVDFLNNNKNFFKKGTIITDTTGIKETLIND
                     VLQIIPDDIDFIFGHPMAGREKRGIDFASEQVFNGANYIITPTGRNNIRNLELVENLI
                     LKLGFKRVKKLTSQKHDEIIAFTSQLPHVMAVALINSDEEDRDTGKFIGDSYRDLTRI
                     ANMNEDLWSELFLGNRDNLLKVIENFESEVNLIKEAIFNNDKSKLIEYFKKSSIRREI
                     LEK"
     gene            16803..18272
                     /locus_tag="L3M60_001636"
     CDS             16803..18272
                     /locus_tag="L3M60_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBN6226313.1"
                     /translation="MLIVRNVSIAFIVLFFFSVFAFFYVANINRVLEYETNNLMTITI
                     TGWIILSFLFLGIIIYILYSKAKSQKTIEKVAYTDFVTGYSNWRKFELDVTNLLKKTS
                     QNNKYAMVIFDIDKFKAINDIYGHKKGNLILKDIADTLNELININETFARVSADNFNI
                     LLTYNKKEDIINIIKKIMVNNELVNLSFGIYEIKDKALSVSVYSDRASLAKSSIKNNS
                     DVNFAFFNDELREKLLLEDKIEKEMEYALESGQFVMYLQPKYNIKLNKFCGSEALVRW
                     QYTEKEVIYPGDFIPVFEKNRFIRKIDMYILEQACKEIRSLFDKGISPLPISVNFSRV
                     DFLKKDFISNIVNVCDRYKIPYGLIEIEITESSMFGDTDILFNVSRNLQDIGFIVAMD
                     DFGSGYSSVNMLKNIPLNVIKLDRGFFVDDKDVDKSQIVIKSIVSLIKQLGIRVVAEG
                     IETKSQIEMLKKANCDIVQGYYFSKPLPIKEFEKLVYKI"
     gene            18305..21742
                     /locus_tag="L3M60_001637"
     CDS             18305..21742
                     /locus_tag="L3M60_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HBN6226314.1"
                     /translation="MIRAKSLKNVLSIVLIFLFTIISFSDFAFKTNVQEEENSKINLR
                     EISLQSSNAIKIKLDSSLSFIKSMANMVSYFNTIDNSKIIPLLANISKESDFQRISIV
                     SPDGISHTSDGKIIDVSNYQYFKKAISGESNISEVVKSPIDGKDTFVIATPIFNKNNK
                     IIGILYGSYYNDKLAEFIDITSFNSEGYIDIFESSGQFVLKSSHTNKLIKSSSNIYDI
                     FKKASFDDGYSYKKIYKDTKNNKSGFTSYEYKGENRYATYTPIGVNDWYVISVIPQYT
                     VRRNIDKINQLAFSLIFKVFLLFIILIIYIIYKNNKSQRDILKANKEISSITNSIPGG
                     VQKCTLGETCKINFLSDGFIELFGYTREEIKTLFNNDFYSLIHKDDVERIKKEISKQL
                     YKNKTIELEYRIITKSGNTVWVLDKGELITEPDGKEYFCCVTVDITRTKQLEQEMEQK
                     NLELEMISENILGGILITELNEDFTIKYTNSGYLSMIGYTREQLENELKYKACDLVFY
                     EDRKKALESIQHQLNTKGIAVYQHRIRRRDGNIIWVSIKGRLIIDDDGIEKGIWILND
                     ITDLKNAEQKLKINEERFRIALKQTSNTIFEYDIKTKSLLNTSKELDKYNIYKITENV
                     PYSLVESGVIHKDFSNDFINMYEKVINGAKKASCVVKMKLPDENYIWNKVTLTTIFDE
                     DGNPVRAIGIQEDISKQKEAEIRYNQEGKYHSAILSEAMITYEINVSKDTFIKGNENW
                     QKPFGIKPDNNYSQMAKTIANSIVHKENKEVFLNTFLCENLLDAYKNNITEIKLEYQR
                     MNQENKMIWVICTMHLIKDSITNDIKALAYVKDIDKQKNEEIRLKLKAERDTLTGLYN
                     KGTTEKLINDFLNSEKASLGMHAFLIVDIDNFKSVNDNLGHVFGDKVLFEVSKHLKPI
                     FRKDDIVGRIGGDEFIVFLKNIQSFENAKKKAEKICDIFRNSYTKNKKTYKISGTVGI
                     SFVPEHGTVFEELYKKADIALYYAKSKGKDKYALYNDCIYQNELENNISDNNIKVANK
                     CEKSNPVLKEVLDSIDNYIYIVNPNTYELLFVNNKVSRSGLEIKLGQTCYKSLLKSDR
                     PCSNCPLNGLEENKHSKFKIETNTFGTNISIASWINCIDGEYNCLIECIDSSLYKNK"
     gene            21759..22505
                     /locus_tag="L3M60_001638"
     CDS             21759..22505
                     /locus_tag="L3M60_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBN6226315.1"
                     /translation="MLNGITLILGIIFTIYYFYLKIVFGGISFSEIFLVVGIILIIYQ
                     IFKKKIKEKKVFYRVLKIIISVFLIVFVVTESLIIFYPKNSLESKSDYLLILGASVKK
                     TIPSTTLKGRLNTALKYLKVNDNCYVVVSGGKGSGENITEAKAMKDYLIKNGIDKNRI
                     IEEDKSTNTYENFKYSKFKIEERSERKLSDLKVKIVTTDFHVLRSKILAYRNGYKNTS
                     FYASKSKLSFVPTYYTREFFALWKTIFFDR"
BASE COUNT         9254 a         2292 c         3885 g         7197 t
ORIGIN      
        1 atatgtcaaa gaattttata aaacatttga tgaagccaaa ggagagtact ttgatggttt
       61 tataataaca ggagctccag ttgagcaaat ggagtttgaa gaagttgatt attggaatga
      121 attagaagaa attatggatt attctaagag taaaactaaa tctacattat atatatgttg
      181 ggcagctcaa gcaagtcttt acaaatacta caatgtaaaa aaacttccat taagtcaaaa
      241 atgttttgga gtatttaagc ataaggtaga taaaaatagt aaaatagtag atggatttga
      301 aaatgaattt tttgctccac attcaagaca tactactgta aatattgaag ctctgaaaga
      361 gaataaagag ttgagcattg tgagtcattc taaagaagct ggtccttata taattacaaa
      421 ttcaagagac gtgttcgtaa tgggtcatag cgagtatgac aaatacaccc ttgacaaaga
      481 gtataagagg gatataaaca gaggagataa aatatcgata cctcaaaatt actatatcaa
      541 tgacgaccct tcagaagagc caactgtaaa atggaaaaaa cattcggaac tactttttag
      601 aaattggata aaaaactatt tgatacaata aattttaata gtaaaatatc aactaaataa
      661 aatataaaaa taataaggta taaagttaat tttaacgatt ctttatacct tattatttta
      721 aaaaaattga ccttataata gtagttttat atataagata ataaaattcc aactgaagtt
      781 acaatattgt aattgacaat agatacttaa tattataata tttaatacat taaacattat
      841 agtattaagt atttatttac aagatacact taagcctaaa gttaatagtt agtgaaataa
      901 agtatttttg taaggagcgt ttgtatggat gagatgagtt ttaagaaaga gctactagag
      961 ttgactagag atataaatat gaaatttaca actttattaa ctaaatttta tcagccactt
     1021 ggtattacag cagttcaagc tttgattttg tctgagttgt gtgaacatgg agagaaaaaa
     1081 ataagcgatt tagggaaaaa cttaaacatg acaaatagta atgtatcagt aatctgtcaa
     1141 aggcttgaaa aaaatggttt tttaaatcgt ataagagata tagaagacca acgaattgta
     1201 aaagtaaaag ttaccaataa atcattggat atacaagaga atatttcaag tagtattttt
     1261 gataattatt ttgaaaatat gactagtgaa aaattgcaag acatggaaga tataatagaa
     1321 ggtttagaga aattaaataa acttttaacc tacattgatt gcaaataaca taaaataatt
     1381 taaaggagaa taaaattatg gatgaacata aaccccaaaa aaggtctgca ctctattatt
     1441 atacagttat aataatagta gtaatgtttt taaatgcttt aatatttcca agtttaatgg
     1501 ataagaaaat ggttgagatt gactatggga agtttcttac aatgttggac acaggaaagg
     1561 ttaaatctgt tgagatagaa aacaatagaa tagcaataaa tccatctagt agtaaggata
     1621 agaatatata tataactggt agaatggatg acccagaatt ggtagataga ctaaaaaatg
     1681 caaaagtaga atttactaag gttattccta aggaaaattc acctctttta aacattttac
     1741 ttacatggat aatacctata ggtattctta tgctatttgg taatttaatg atgaagtcta
     1801 tgcaaaagcg aatgggtggt aatgctatgc aatttggaaa aagcaatgcc aaaatgtatg
     1861 tatctgctca aagtggaaaa agatttaatg atgtagctgg tcaagatgag gcaaaagaag
     1921 ctttaagtga aatagtagat ttcttacaca atccagaaaa gtataaaaaa ataggagcac
     1981 aaatgcctaa aggtgcactt ttagtaggac ctccaggtac aggaaaaaca ctacttgcaa
     2041 aagccgtagc aggtgaagca aatgtaccat ttttctcaat atctggttct gagtttgttg
     2101 agatgtttgt aggaatggga gcttctagga ttcgtgattt atttaaacaa gcaaaagaaa
     2161 aagctccatg tatagtgttt atagatgaga ttgatactat tggtaaaaag cgtgataatg
     2221 gaagtggact aggtggaaat gatgaacgtg aacaaacttt aaatcagttg ctaagtgaga
     2281 tggatggttt tgatggtgga attggagtag taatacttgc tgctacaaat agacctgaca
     2341 gtcttgacaa agcattactt cgtccaggaa gatttgatag acgtatacca gttgaacttc
     2401 cagaccttgg aggacgtgaa gctatattaa aggtacatgc tcaaaatgta aatgtaagtt
     2461 cagatataga ttataatgca attgcaaggt ctacatcagg agcatcaggt gcagaacttg
     2521 caaatattgt aaatgaagca gcacttttag cagttaagtc tggtagagat atagtagagc
     2581 aacaggattt tgatgaatct gttgagactg taattgctgg gtatcaacgt aaaggagctg
     2641 taatttcaga caaggaaaag aaaattatag cgtatcatga aataggtcat gcacttgttg
     2701 ctgctatgca aaaacattct gcacctgttc ataagattac tataatacct agaacttcag
     2761 gagcattggg atatactatg caagttgctg aagatgagag cgtccttatg tctaaagaag
     2821 aagcaattga caaaataact acttttacag gtggtcgtgc tgcggaagag gttattttta
     2881 acacttgtac atcaggagca tcaaatgata tagaacaagc cactaaaatt gcaagagcta
     2941 tggtcactcg tcttggtatg agtgaagaat ttgatatggt tgctcttgaa actgtaagca
     3001 atcaatattt aggaggagat acttcccttg cttgttcttc cgacactgct gctaaaatag
     3061 atgaagaagt atttaaaata gtaaaaactt gtcataaaaa tgcaattgaa atcttagaaa
     3121 ataatattga taagttgcat gaacttgcta agtatttatt agaaaaagaa actataacag
     3181 gtgatgaatt tatggaaata cttaacttaa actaaaatga ttatacacaa agtaaaaatg
     3241 cctattgtta ggcattttta ttttgtaatt aagtttttta aattcatatt taaatcttta
     3301 cataatctta atcactaaaa atacatctta actacaattt tatattggaa ttgatattat
     3361 aatagtaatg aatgatgtaa ggaggttttt taatggatag accgaatcct gatattctat
     3421 tagaaaaaat aaaaaatgaa gaagaaaaac taagtagagg tcaattaaaa atattctttg
     3481 gttatgcatc aggagtagga aaaacctatt ctatgttgga atctgcacaa aatttaaaga
     3541 aagtaggtgt tgatgtagta gttggatata ttgaaccaca cactagacca gaaaccttgg
     3601 ctttacttga tggattggaa attctccctg taaaggagat agaatataaa tctataaaat
     3661 taaaagaatt tgacttagat gctgctttag caagaaaacc agaagttata ttagtagatg
     3721 aatttgccca ttctaatgtt gctgggttaa ggcatacaaa aagatggcaa gatatagagg
     3781 aattgctgtt agcaggcata aatgtctaca ctactgtaaa tgtacaacat ctagaaagtc
     3841 taaatgacat tgttgagata attacaaacg tatcagttaa agagactata ccagataagt
     3901 ttttggatac aaatacacag ttagaactta tagatgttga acctgatgta cttcttgaaa
     3961 ggttcaatga aggtaagata tataagaaag aacaagctac tagagcaaaa aataattttt
     4021 ttataaaaga taacctagtt gcacttaggg aaattgcact tagaaaaaca gcagaaagag
     4081 tcaataaaga agttcaaatg accagactat caaagggtga tgtaactgta atacctacat
     4141 cagatacgct tcttgcatgt ataagtccat ctccatcttc agcaaaagtt attagaactg
     4201 catccagaat atcagacagt acatttgcaa aatggatagc tttatatgtt gagactccaa
     4261 acacagcaag acttccattt gacgaacaaa aacaactaca aaataatcta aaactagcta
     4321 agaagctagg tggagaaata attgttttac atggtgaaaa tataatagag caaatactta
     4381 gaattgcaaa actgagaaat gtaacaaaaa ttgtaatagg tagaaatcat tcaagtaata
     4441 aaaggtttag taaaaagttt aaaaaagata tagttgacaa attaattgat gaagttgatt
     4501 atatagatat acatataatt ccatataaat ttgcctcaga tgttaaatac aaacctaaaa
     4561 aggataaagt gtcaataaaa tctaaattta aaattagtaa agttgatttt ataaaactta
     4621 tatttattac tttgatgata agtattttag catatgcagt acaaagtatg ggctttataa
     4681 gagaaaatat attgttaata tatatgttgg gtgtggtttt agtttctatg tggactaaag
     4741 gttatagtac aggtataata agttctgtat ttaatatagt tctgttaaat tattttttta
     4801 cagcaccatt atatacatta tctatagcag attcaaacta tatagtaaca ctagttgtat
     4861 tttcaatagt aggtataata actagtactc ttacatctaa gatacaacat gaagcagaaa
     4921 ctgcttctaa aagagaagaa aatacaaaaa tgatatatca aataattaag ggatttttaa
     4981 gattatcaaa taaagacgat ataataaaca aaggtataga acttttgtct cttagcttaa
     5041 gtagagatgt gatttgttat ttaaatgtag ataagtataa caaatcgaag ctttataaaa
     5101 aaaatactaa taataaggat aaaaatgatt tggatagtga agatgaaaaa gctgttgcaa
     5161 gttgggtttt atccaattca acagtagcag gaaatgatac ggatacactt cctggttcaa
     5221 agggatacta tataccaatt gtagggatga atttaacttt aggagtagta ggaatatcat
     5281 gtgttgattc aaaattggat acagaagata taagcttaat tgaaacaata attgctcaaa
     5341 tggcaatagc acttgataga gaaatcttgt cagaagctaa agaaaatacc aatctcgaga
     5401 ttgaaaggga aaggctaaga agtaatcttt taagagcagt ttcgcatgac cttagaactc
     5461 cactagcagg aatatctggc gcagtaagta caattataaa aaacaagggt actataggtc
     5521 aagatataat agatgaattg cttagtggtg tatatgaaga tactcaatgg ttaataagac
     5581 ttgttgaaaa tttacttagt atgaccaaga tagatgaagg aaaattggaa gttaaaaaac
     5641 attctgaact agttgaggaa attatatcag aagcattaca aaaaataaaa aaaagaattg
     5701 aaaatgcact tatagatatc aatataccag aacagatatt atttgttccc atggatgcaa
     5761 aattaataga acaagtttta attaatttaa tagacaactc tttgaaatat tcaaaggaag
     5821 attgtaagat agagataaat gtatatgaga aagatgatta tgtttggttt gaagtatctg
     5881 ataatggtcc tggtatatca aaggaactta aaaagcatat ctttgacaga ttttttactg
     5941 gagaagaagg agctaaagat tccagaaagg gtgttggact tggattatca atttgtaagt
     6001 ctataattca agctcataaa ggtgaaatta tggtagaaga caataaagat aagggctcaa
     6061 cttttaagtt tagtttgcct aaggaaaatg aatagtttta aattttaaaa ttagttttgg
     6121 aggaagaaat gagtaaaaaa cttatattgt tagtagagga tgataagaca ataagaaaat
     6181 ttatatctac tgcactttta acacaggatt atgatgtcaa ggaggctata actggaaaag
     6241 aaggtatatc tattgctgtt tcatattctc cagatgttgt tctcttagat ttaggcttag
     6301 aagatatgga tggaatagag gtaataaaag ctatcagaca attttcaaat ataccaataa
     6361 ttgtagtttc agctagagag caagatagag ataaggtaga agtatttgat gcaggggcag
     6421 atgattactt aacaaaacca ttctctatag tagagttatt agcaagagta agagtcgcat
     6481 ttagacattc acaagtagaa gttcaacaaa aagatgatgt taaatctacg tttgaagtag
     6541 ataaattatt gattgatttt gataaaagaa aagttattgt tgatgatatt gaagtccatt
     6601 tgacacctat tgaatataat attctttcat tacttgcaaa gcatcatgga aaagtgctta
     6661 ctcataactt tataataaaa gagatatggg gtagtgttat agggaatgag actaagtctt
     6721 taagggtctt tatggcaaca ttaagacgaa aaatagaaaa acaacctgca aacccaagat
     6781 atataatcac agaagttggt gtaggataca gattaaatga tgaatagttg taatatatat
     6841 taattattag actatgtttt tattaatata ttaaaattat atagtttagt taaaataaga
     6901 atgaagtgta tgtgctgttg tcagtttagc acatgcattt tattttttaa atttttttag
     6961 tatcaataat tttttagtat taataatatg aaatatgttt taaaataagt atatggtata
     7021 atctgtaata aaagctttgg ttaaataata aatgtactta acacttgtct tataataaat
     7081 ttaatattta tattaaataa taattattaa aatagtgtaa aataattaca gtaaggatta
     7141 taaaaaattt ttagaatgta aattgaaatt taggagtaga tgtatgtata tactgaattt
     7201 aaatacaaga gagactattg aagattttag agataaattt tatgtagcag aaaattctta
     7261 tcttatatta agtgctccaa aaaatttaaa attgcttaaa gaaacattgg atatagatga
     7321 aataactttt aatgattgtc ttaaatttga tgaaataacc aaacttgatt tatttgataa
     7381 ttacgatttt ttaagcctaa atacatttga attaagagat ggtgaagctg ttatagaaga
     7441 ggtaaatatg tatttgtctg ataattttat attggttgta gttaatgaag aacacttttt
     7501 atttgaattt gtaaaaaata ttattttgaa gaactctcaa cttgaaaaaa atccagttat
     7561 taacctattt aagataaact acttgattct cagagaagtt ataaaaaatg gttttgagtc
     7621 tttggaaaaa gttgaagagt tgattcttca aatagaagat gaaatgatgg acaacattaa
     7681 caagaatcat gtatctagaa taagtgatgt aagaggatta acaaggatta tagttaagaa
     7741 tactagacct cttttgtata ttggggatag aatagttaaa gaaaatataa gatatttaaa
     7801 atattcaaat gtaaaaaaat ataatttaga aaattttcaa ggtatagatt ttggaattga
     7861 taaactatac agtttcgcat tgtcaacacg agagttagca gataaattat tagatattta
     7921 ttcttctaga gtaggtgaaa aaacaaataa tttaattaca aaattaacct tattaacagc
     7981 catatcagca ccacttacaa ttataactgg tatatatgga atgaacttta gatatatgcc
     8041 agaattaaac tggatttatg gatatccagc aactttattt tttatgttat gtataatttt
     8101 tgttggaatt ataatattta aaataaaaaa attattgtaa attacagatg atttttaatt
     8161 aattttataa aaatacatca attgagaagc cgatttaagt aaataaaaaa atattttaaa
     8221 ttgaaaatat ttatagaaaa tattgacaat tgtattttct ttgttataat tagtaaacaa
     8281 gaagaataat aaattataaa aatggaggat aatactatga aagggatact aaaagaacta
     8341 gcagcaaaac tttatagctt tttttacttt atgttctggg gcttttattt tagcgctggc
     8401 tatttctttt acaaaaatat atgtaattcc tttctggtga ggccttattt tagtaatttt
     8461 gagcataagt aaattttata aaaatgatta ttttatatat ttatgtaaga tttaatgctt
     8521 gatagattat tttagagaac tcattagtga gttctctttt ttattttaaa tataaaatta
     8581 taatattggg aggtagtaaa aatgatagta gtattaaaaa taggagcaga taaaaatgaa
     8641 gttaaaaaat taattgaggc tattgggaga gaaggagtag aagttaatcc catagatggg
     8701 acagaattaa ctgttttagg tttagttgga gatactagta aaatagatgc caaaagaata
     8761 gaagcaaata agatagttga aaaagttatg catgtagtag agccttttaa aaaggccaat
     8821 agaaaatttc atccagaacc ttctataata aatgttaatg gaatggaaat tggttcgaaa
     8881 aaaattgcta tgatagctgg accttgttct gttgaaacag aagagcaaat agtatctata
     8941 gctgaggatg tgaaaaaatc aggtgcagga tttttgagag gtggagcatt caaaccaaga
     9001 acatcaccat acgctttcca aggattgaga tatgatggat tggacttatt aaagaaagca
     9061 aaagaaaaaa cagggcttcc tatagttaca gaaataatgt caacacaaga tatagatata
     9121 tttgaagaaa atgtagatgt aattcaagtt ggagctagaa atatgcaaaa ctttgattta
     9181 ttaaaagaat tgggtaaaac aaataaaaca atacttttaa aaagaggatt atctgctaca
     9241 attgaagagt ggttaatgtc agcagagtat ataatggctg gaggaaatga aaatgtagtt
     9301 ctttgtgaaa gaggtataag aacttttgaa acatacacta gaaatacttt ggatttaagt
     9361 gcaattttag ctgtgaagaa acttagtcat ttgccagtta tagttgatcc aagtcatgca
     9421 gcaggcaaat catggatggt tgactcttta tctaaggcag caattgcagt tggagcagat
     9481 ggtctgataa tagaagttca taatgaccca gcacatgcac tttgtgatgg aaaacaatca
     9541 attaaaccaa atgaatatga tgaattaata agtgaattaa agactattgc atcagcagta
     9601 ggaagagaaa tataatctat aaaaggatgg ttaaacgtgg aagaaaaact attagtaaat
     9661 ttaaaagaaa atagctacaa tatatttatt aaaaaaaata tactaaacaa tgtaggagag
     9721 gaaataaaaa gaatatattt tggaaataag atttttattg taactgatga aaatgtagca
     9781 aaattatacc tggataaatt aaaaaataat ttaagtgaat ctggatttga aataaaatct
     9841 ttaatattaa aatctggtga agaaacaaag tcatttaata ctcttcctaa aatatacgat
     9901 agtttacttg actttaagtt aactagaaaa gatttgatta ttactttagg aggaggggta
     9961 ataggtgatt taggtggatt tgcagcagca acttttttaa gaggtataaa ttttattcaa
    10021 attccaacat ctttattagc acaagttgat agttctgtag gtggaaaagt tggagttgac
    10081 cttgagagag gtaaaaattt agtaggaagc ttttatcaac caaaagctgt atttattgac
    10141 ccaaatgtat tattatcact tccagaaaaa ttctatagag atggaatggc agaagttata
    10201 aaatatggat gtataaaaga taaaactttt tttaataaac ttttaagttt aaaaaataga
    10261 gaagaagtaa tgcaacatat agaatacatt atacataaat gttgctttat taaaaaaact
    10321 gtagttgaag ctgatgaaaa agattttggg gatagaatgc ttttaaactt tggtcataca
    10381 ttaggtcatg ctattgagaa acactacaat tttacaggtt atactcatgg agaagctgtt
    10441 gccataggaa tgtataagat aactttagaa agtgaaaaga taggaattac aaaaaaaggt
    10501 acatcagaac ttataaaatc aattttaatg aattataatt tgccttatga gattgaacta
    10561 agagataata aatctatagt agaagctata tctcttgata aaaaaaatct aggtgataca
    10621 ttaaaaataa tacttttaaa agatattgga gagagtatta tatataatac tacttctaaa
    10681 ttttttcagg aggtaagata atgggaagtc taaaaatata tccaagtaaa ttatctggag
    10741 atgtaaaaat accaccatca aaaagtattg cgcatagggc agttatttgt tcatctctaa
    10801 gcaatggaaa aagtagaatt tctaatatag atttttctga tgatataata gctactataa
    10861 gagcaatgac atcattagga gctattatag aaaagaaaga agatatttta gaaatttcag
    10921 gaattttttc gaaggaaggt attttaaata gagagaatca gttgaatcaa cctaaattga
    10981 caatagattg taatgaatca ggctctacat tgagattttt agtacctata tctttagcat
    11041 ttgatggtgt aaaaaggttt ataggtagag gtaatcttgg gaaaaggcca cttgatactt
    11101 attatgagat atttaacaga cagaatataa aatatagtta caaagaaaat cagttagatt
    11161 tgattatatc aggtaagctt aaaccagatg agtttagagt aaaaggaaat ataagttctc
    11221 agtttatcac aggtctatta tttatattgc ctacattaga aagtgattca aagataataa
    11281 ttactactga gttagaatct aaaggatatt tagatttaac tctttcaact atgaaagatt
    11341 ttgggataga aattataaac aataattata aagaatttat tataaaagga agtcaaacgt
    11401 ataaagcgag agattataaa gtagaaggag attattctca aggagcattt tatttatcag
    11461 cagatgctat aggagaagat attaatatat tggaccttaa agaagattct cttcaaggag
    11521 atagtgaagt agttgaaatt ttatctagga tgggaatgga aatattaaga gagggtaaca
    11581 aaataaaagg aataacaaat ggtttaaata gtactttgat agatgcatct caatgcccag
    11641 atataatacc tgtattatct gtagtagcag ctttaagtag agggaaaact acaataatca
    11701 atgctggtag acttagaatt aaagaatgtg atagacttca tgcaataaat attgagttaa
    11761 gtaaacttgg agcgaatata gaggagaaag aggatagttt gataatcgaa ggagtaagta
    11821 agttaaatgg gggagtagag gtgtggagtc acaaagacca tagaattgct atgacacttg
    11881 caattgcttc ttgtagatgt gataaaccaa taatactaaa agattttgag tgtgtatcaa
    11941 aatcttatcc acattttttt aaagattttg agatgttagg aggacgaatt gatgagtgga
    12001 atatggggaa ataatttaaa aatatccatt tttggagaat ctcatggaaa tgctataggt
    12061 ataaatatag atggtcttcc ttcaggagtg gaacttgacc ttgagaagat tgatagagaa
    12121 atgaagagaa gagcacctgg aaaaaattct atatctactt caagaaatga aagtgatata
    12181 ccagaaattc taagtggata ttttaatgga agaactacag gaacgccatt atgtgcaatc
    12241 ataagaaata gtgatactcg ttcaaaagat tatggagaac ttaaaaatct aatgagacca
    12301 ggacatgcag atttcacagg aaatgtgaga tactctggat ttaatgacta cagaggtgga
    12361 ggtcattttt caggtaggat aacagctcca ttagtatttt gtggggctat atgtaaacaa
    12421 attttgtcac aaaaaggaat agaggtagga gcacacataa aaaaaattaa aaatatagaa
    12481 gatatgagtt ttaattatgt aaatatttca aaacaacagt tatcaaattt gcaaacttta
    12541 gaattacctt tacttgattt atcaaaggaa gaaaaaatga aaaatactat tatagatgca
    12601 aaaaatcaaa gagattcagt tggtggtata attgagtgtg cagtagtagg tgttaatgta
    12661 gggtttggaa atccattttt tgattctgtt gaatctactt tatcacattt attgttttct
    12721 gttcctgctg taaaaggcgt agagtttggt ttaggttttg aacttgcaga tatgtatggt
    12781 tctcaaagta atgatgaaat gtattatgaa ggaaatcaag ttaaaagcaa aacaaataat
    12841 aatggaggaa ttattggagg tattacgact ggaatgccaa taattttcaa ggtagcaatc
    12901 aagccaactc catcaattag tagacaacaa aacacagtta atataaaaga gaaaaaggac
    12961 gatattttat ctattaaagg cagacatgac ccttgtatag ttcaaagggt tatacctgta
    13021 atagaagcag taacggccat tggaattttt gatttgatga aaggacgata ataatggagg
    13081 atttaacgag atatagaaat gagattgaca atatagataa agagcttatt caattatttg
    13141 aaaagagaat gaatacagtt cttgaaattg ccagacataa aatgaaaaat aacactacta
    13201 tacttcaaaa agatagagaa gaaaaagtac taagtagagc cgtagataat ttagacaata
    13261 aagcctattc acaagaaaca atacaatttt ttaattctat aatggaaatt agtagaggat
    13321 tacaaaaaag gttgatggat aatgatagtg agcagaaaat tgaatttagg ctagactcat
    13381 caaagaagaa atttgacttg tcaagtgtaa ataaatataa gtctttaaaa gaagaattaa
    13441 ataaaaaaaa tatcctagta ggttttcctg gaaaatcagg ttcatttaca gaagaagcac
    13501 taaataaatt cttcaacaaa aaaacagcaa aaaaacaatt taaagaattt gaagatgttt
    13561 tcatagcact taaaaataaa aaaatagact atggaataat tcccatagaa aattcatcca
    13621 ctggtgctat ctctgaaact tatgaccttt taagaaaata cggtttttat atagttggag
    13681 aagaatgcat aaaaatagac caaaatttaa ttgggataaa agatacaaaa cttaaagata
    13741 taaaagaaat atattctcac ccacaaggta taggacaatg tagtgaattt ttaaaacaaa
    13801 atagtgcatg gaagttaata ccatttaata acacagcaac aagtgctgaa cttgtaaaaa
    13861 aacttcaaga taaaacaaaa gcagcaattg caagtaaaaa agctgcaagt atttatggat
    13921 tagaaataat aagtccttgt ataaatgata ttacaaacaa ctatacaaga tttgtagtaa
    13981 tcagtaatca aatacacatt gaagaagagt caaacaaaat gagtgtagtg ttttctgttg
    14041 aacataaagc aggaaagctt tataaagtac ttggatactt tgctgaaaat aatataaaca
    14101 tgacaaaaat agaatcaaga cctatgaaaa atacctcatg gagatatttc ttttatatag
    14161 atttcgaatg ttctatatac aattctaaag tatacaattt gttagaattg atagaactta
    14221 atacagcgta ttttaaattt atgggagtat ataaaaataa aactaaatag ataagataaa
    14281 tttttattta attattagga ggtgtaaata tgaatttttt tggtttagta ggtgaaaaac
    14341 tctctcatag tgtttcccct caaatccaca agagagtatt cgaaatattg aatattgaaa
    14401 gtgcctataa aaactttgaa atttcaaaag aagatgtatc taagctagct agtgctataa
    14461 aattattagg aatacaagga gtaaatgtta ctgtaccata taaagaacgt attatgaaat
    14521 atcttgattt tatatcacca gaggcaaagc gtataggagc agttaataca attttactca
    14581 gagaagatat gttatatggt tataatacag attactttgg tcttgatagt atgtttaaaa
    14641 tggctaatat tgatgttcaa ggaaaagtgg cagttatatt aggaactggt ggtgcttcta
    14701 aagctgctat tacatatctt ctcgattctg gaatagagaa ggtttatgtt tcaactagaa
    14761 aaaaagatga taaaaaatta ttaaatagta aagctatatt ggtagattat gaagaactta
    14821 aatgtataac aggagatata atattaaatg ctacaccagt aggtatgtac cctaatgtag
    14881 gaatttcacc agtgtcaaaa tctattatac aaaattttga tacattagta gatttaatct
    14941 acaatcctgg agaaacagag tttttaagaa ttggtaatag tattggtaaa aaaacttgtg
    15001 acggtttata tatgttggta gggcaagcga taaaatctca ggaaatttgg caaaatgcta
    15061 aaatagataa tagtatttta gatgttatat ataatgaatt aaaattagaa tttttgtaag
    15121 ggagattgtc atgacaaata aaacaaaaga aaaattaata ttaataggta tgccaggtag
    15181 tggaaagact acaattggaa aacttttggc tagagaatat aattgtagtt tttgtgatat
    15241 ggatgattat atagttaaaa tagcccaaaa aagtatagca gagttgtttt cagaaggaga
    15301 agatattttt agaaactatg aaactcaagc atgtagggaa ctttctataa gtaataaagc
    15361 agtgatatct acaggtggag gtgtaataaa aaaggatgtc aatatggaaa tattaaaaga
    15421 aacaggtata ataattttta ttgatagacc tatacaaaag attttagaag atataaatat
    15481 taattcaaga ccgttattaa aaaacggaaa agatagattg tataatcttt ataatgagag
    15541 aataaatcta tataaaaaat tttcagatat agagatttta aatgacaaat cattaaatac
    15601 tgctgtatat aatattataa atgcagttag tgaaaaattt aaatttgaat ttaaagaaaa
    15661 ataatctagg aggaaataaa aataatgaat attgttattg taggattagg agttatagga
    15721 ggttcttttg ctaaagcatt aaaaaaagct ggatatgaaa atgtatttgg agtagatgta
    15781 gatttagaaa ctttaaaaaa agcagaaaaa gcaaaaatca taaaaaaagg atgtactaca
    15841 ggtaaagagt tatttaaaaa agctgattta attatactat ctatatatcc tagattagtt
    15901 gtagattttc taaataataa taaaaacttt tttaaaaaag gaactataat tactgatact
    15961 acaggaataa aagaaacact tatcaatgat gtgttgcaaa ttattccaga tgatatagat
    16021 tttatatttg gccatccaat ggcaggcaga gaaaaaaggg gaattgattt tgcaagtgaa
    16081 caagttttta atggcgcaaa ttacataatt actccaactg gcagaaacaa cataaggaat
    16141 ttagagttag ttgaaaattt aattttaaaa cttggattta aaagagtaaa aaagttaact
    16201 tcacaaaaac atgatgagat tatagctttt acttctcagt tacctcatgt tatggcagtt
    16261 gctcttataa atagtgatga agaagataga gatacaggta aatttattgg agatagttat
    16321 agagatttaa ctagaattgc aaatatgaat gaagacttat ggagtgaact ttttctaggg
    16381 aatagagata atttactaaa agttatagaa aactttgaat cagaagtaaa cttaataaaa
    16441 gaagctatat ttaacaatga taaaagtaaa ttgatagagt attttaaaaa atcatcaata
    16501 aggagagaaa ttttagaaaa ataaatattg atatttaagc tgtatataga tgggtataaa
    16561 tataagcgat agtgtattta attgtattaa taaatatgga ttcaatataa ttaaatatgc
    16621 tattgatata tttgtaccct taaatattgt tatcttttaa atgataatga catttaccta
    16681 aatactcttt attaacaatt tataaaactt aattgtaaaa aatacttatt aatagtaaaa
    16741 taaagtgaat caattatatt ataattgcta aagagggtga ggtgtagcta tttgaaaaaa
    16801 atatattaat agtaaggaat gtatcaatag catttatagt actattcttt tttagtgtat
    16861 ttgcattctt ttatgtagcc aatattaata gggtgttaga atatgaaaca aataacttaa
    16921 tgacaattac aattactgga tggattatac tttctttttt atttttaggt attataattt
    16981 acatattata ttcaaaagca aagagtcaaa aaactataga aaaagtagca tatacggatt
    17041 ttgtaacagg ttattccaac tggagaaagt ttgaattgga tgttacaaat ttattaaaga
    17101 aaacatcgca aaataataaa tatgcaatgg tcatatttga tattgataaa tttaaagcaa
    17161 ttaatgatat atatggccat aaaaagggaa atttaatatt aaaagatatt gcagatactt
    17221 taaatgaatt gattaatatc aatgaaactt ttgctcgtgt aagtgctgat aattttaaca
    17281 tattattaac atataataaa aaagaggata taataaatat tatcaaaaag attatggtaa
    17341 ataatgaact tgtaaactta tcttttggaa tttatgaaat taaagataag gctctatctg
    17401 taagtgtata tagtgacaga gcctcacttg caaaatcatc aataaaaaat aatagtgatg
    17461 taaactttgc cttttttaat gatgaattaa gagagaaatt attacttgaa gataagatag
    17521 aaaaagaaat ggaatatgct cttgaaagtg ggcagtttgt aatgtattta caaccaaaat
    17581 ataatattaa attaaataaa ttttgtggaa gtgaagctct agtcagatgg caatatacag
    17641 aaaaagaagt catatatcca ggtgatttta ttcctgtatt tgaaaaaaat aggtttatta
    17701 gaaagattga tatgtacata ttagaacaag cttgtaaaga aatccggtct ttatttgata
    17761 aaggaatttc acctttacct atatctgtaa acttttcaag agttgatttt ttaaaaaaag
    17821 attttatttc aaacatagta aatgtatgtg atagatataa aataccatat ggtttaatag
    17881 aaattgaaat tactgaatct agcatgtttg gagatactga tattctattt aatgtgagta
    17941 ggaatctaca agatattggc tttatagttg caatggatga ttttggttca ggatattctt
    18001 ctgtaaatat gcttaaaaat ataccattaa atgtaataaa attagataga ggattttttg
    18061 ttgatgataa agatgttgac aagagtcaaa ttgttataaa aagcatagtt tctctcatta
    18121 agcaattagg aataagggtt gttgctgaag gaatcgaaac aaaaagtcaa atagaaatgt
    18181 taaaaaaagc aaattgtgat attgttcaag gatattattt ttcaaaacca ttaccaatta
    18241 aagaatttga aaaattagtc tataagatat agtcagcgag tcttaaatgg gaggtggtga
    18301 ttaagtgata agagcaaaat cgttaaaaaa tgtactgagt attgttttaa tatttttatt
    18361 tacaataatt tctttttctg attttgcatt taaaactaat gtccaagaag aagaaaatag
    18421 taaaattaat ttaagggaaa tatccttaca aagttccaat gctataaaaa taaaactaga
    18481 cagttctttg tcctttatta aaagcatggc aaatatggtt tcgtatttta atactataga
    18541 taactcaaaa ataattccat tattagctaa tatatcaaaa gaatctgatt ttcaaagaat
    18601 ttctattgta tctcctgatg gaataagcca taccagcgat ggcaaaatta ttgatgtttc
    18661 aaattatcaa tattttaaga aagctattag tggagaaagt aatatatctg aagttgtaaa
    18721 atctccaatt gatggaaagg atacttttgt tatagctact cctatattta ataagaataa
    18781 caaaataatt ggtattttat atggttctta ctacaatgat aaattagctg aatttataga
    18841 tataacaagt tttaatagtg aagggtacat agatatattt gaatctagtg gacaatttgt
    18901 tctaaaatct tcacatacaa acaagttgat taagtcaagt agtaatatat atgatatatt
    18961 taaaaaagct tcttttgatg atggttatag ctataaaaaa atatataaag atacaaaaaa
    19021 taacaagagt ggctttacgt catatgaata taaaggagaa aatcgctatg ctacttatac
    19081 accaattggt gtaaatgatt ggtatgtaat ttctgtgata ccacaatata ctgtaaggag
    19141 aaacattgat aaaataaatc aattagcatt ttctcttatt ttcaaggtat tcttgttatt
    19201 tattatactt ataatatata ttatttataa gaataataaa tctcagagag atatactaaa
    19261 agctaacaaa gaaatctcat ctattacaaa tagcattcct ggaggagtac aaaaatgtac
    19321 cttgggtgaa acatgcaaaa tcaatttttt aagtgatgga tttatagagt tgttcggtta
    19381 tacaagagaa gaaataaaaa cattgtttaa taacgatttt tatagtttaa tacataaaga
    19441 tgatgttgaa agaataaaaa aagaaatttc taaacaactt tacaaaaata aaactattga
    19501 attagaatat agaattataa caaaaagcgg taatacagta tgggttttag ataagggaga
    19561 gcttataaca gaacctgatg gaaaagaata tttttgttgt gtaactgtag atattactag
    19621 aacaaaacaa ttagaacaag agatggaaca aaaaaatcta gaacttgaaa tgatatcaga
    19681 aaatatttta ggtggtatac ttataacaga acttaatgaa gattttacta taaaatacac
    19741 taactctggt tatctttcaa tgattggata tacacgtgaa caattagaaa atgaacttaa
    19801 atataaagca tgtgaccttg ttttttatga ggatagaaag aaggctctgg aaagtataca
    19861 acatcaatta aatacaaaag gaatagctgt atatcaacat agaataagaa gaagagatgg
    19921 taatattatc tgggtttcta taaaaggaag gcttattatt gatgatgatg gtattgaaaa
    19981 aggtatttgg attctaaatg atataacaga tttaaaaaat gcagaacaaa aacttaaaat
    20041 aaatgaagaa agatttagaa ttgcattaaa acaaacatca aacactatat ttgagtatga
    20101 tataaaaacc aagtcacttt taaatactag caaagagctt gataagtata atatttataa
    20161 aatcactgaa aatgttccgt attctttagt agaatcagga gttattcata aagattttag
    20221 taatgatttt ataaatatgt atgaaaaagt aataaatgga gcaaaaaaag caagttgtgt
    20281 agttaagatg aaacttccag atgaaaatta tatatggaat aaagttactc taactactat
    20341 ttttgatgaa gatggtaatc ctgtaagagc aattggtata caggaagata taagcaaaca
    20401 aaaagaagca gagataagat acaatcaaga agggaagtat cattcagcta tattgtcgga
    20461 agcaatgata acttatgaaa taaatgtatc aaaggatact tttatcaaag gcaatgaaaa
    20521 ttggcaaaaa ccatttggaa ttaaaccaga caataactat tctcaaatgg caaaaacgat
    20581 agcaaatagc attgtacata aggagaataa agaagtattt ttaaatacat ttttatgtga
    20641 aaatttatta gacgcatata aaaataatat aactgaaata aaattagaat atcaaagaat
    20701 gaatcaagaa aataaaatga tttgggtcat ttgcacaatg cacttaataa aagattctat
    20761 tacgaatgat ataaaagcac ttgcttatgt taaagatatc gacaaacaaa aaaatgaaga
    20821 aattagatta aaattaaaag ctgaaagaga tactcttaca ggattatata ataaaggtac
    20881 tacagaaaaa ttgattaatg atttcttaaa ctctgagaaa gcaagcttag gaatgcatgc
    20941 atttttaatt gtcgatatag ataattttaa atctgtgaat gataatttag gtcatgtatt
    21001 tggagataaa gtattatttg aagtttctaa acacctaaaa cctatattta gaaaagatga
    21061 tatagtaggt cgaattggtg gagacgaatt tatagtattc ttaaaaaata ttcaatcttt
    21121 tgagaatgcc aaaaagaaag cagaaaaaat atgcgatata tttagaaatt cttatacgaa
    21181 aaataaaaag acatataaaa tatctggaac agttggaatt tcatttgtac cagaacatgg
    21241 aactgtattt gaagaacttt ataaaaaagc agacatagca ctttattatg caaagagcaa
    21301 aggaaaagat aagtatgcac tttataatga ttgcatctac caaaatgaac ttgaaaataa
    21361 tataagtgac aataatataa aagtagcaaa taaatgtgaa aaatccaatc cagtgcttaa
    21421 agaagttctt gattctattg ataattatat atatatagtg aatccaaata cttatgaact
    21481 tctttttgtc aataataagg tgtctcgctc aggtttagag ataaaattag gacaaacctg
    21541 ttataaatca ctattaaaat ctgatagacc atgtagtaat tgtcctctta acggattaga
    21601 agaaaataaa cattcaaaat tcaaaataga gacaaataca tttggtacaa atatcagtat
    21661 agccagttgg attaattgca tagacggaga atataattgt cttatagagt gtatagattc
    21721 ttctttatac aaaaataaat aaaaaggact atgaagatat gttaaatggt ataactttga
    21781 tacttggtat aatatttaca atttactatt tttatctcaa aatagttttt ggaggaattt
    21841 catttagtga gattttctta gttgtaggaa ttatactaat tatctatcaa atatttaaga
    21901 aaaaaatcaa agaaaagaag gttttttata gagtgttaaa aataataatc agtgtttttt
    21961 taatagtatt tgtagttact gagagtctaa ttatttttta tcctaaaaat agtttagagt
    22021 caaaaagtga ttatctcttg attttaggag cttctgtaaa aaagactata ccaagtacaa
    22081 ccctaaaggg tagattaaac acagcattga aatatctaaa agttaatgat aattgttatg
    22141 tagtagtatc tggtggtaaa ggaagtggag aaaacataac tgaggcaaaa gctatgaaag
    22201 attatcttat aaaaaacggt atagataaga ataggataat agaagaagat aaatccacta
    22261 atacttatga gaattttaag tattcaaaat ttaagataga agaacgtagt gaaagaaaat
    22321 taagtgattt aaaagtaaaa atagtaacaa cagattttca tgttcttagg agcaaaatat
    22381 tagcatatag aaatggatat aaaaatacgt ctttttatgc tagtaaatca aaactttcat
    22441 ttgtacctac ttactataca agagaatttt ttgcattatg gaaaacaatc ttttttgata
    22501 gataataaat agaaggacta gatataaatt ataaatttat atctagtctt ttttatgtag
    22561 aaagtaagag ttaaaaatta ttttttcaaa gcttcctgcc attaaattag aaaatttagt
    22621 ctcagtat
//
LOCUS       DAFNNB010000049        22512 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0049, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000049 DAFNNB010000000
VERSION     DAFNNB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22512
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0049"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(87..998)
                     /locus_tag="L3M60_001639"
     CDS             complement(87..998)
                     /locus_tag="L3M60_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HBN6226316.1"
                     /translation="MIKKLDDILDQLKGGEKIVLSVAAAHDKEVLMAIKDAVERNIIT
                     PILVGNEGKIREISKEIGFDLSGIKIVDKDDIKECAEIAVKLVSSKEADFVMKGLLDT
                     SVILKEVLNKDYGLRTDSLLSHVMVYELEKYHKLLITTDGGMNIAPDYEQKVKILKNS
                     IKAAKALGMETVKVACLAAKEKVNPKMQATVDADMLAKACKEGEFGDNVIVEGPLAFD
                     LAVSKEASEIKGFKSEVSGDVDIILVPTIEVGNGIGKAFTYMADSKSAGIIMGAKAPI
                     VLVSRADSHESKLYSIAYGAIVAKNMK"
     gene            complement(1113..1691)
                     /locus_tag="L3M60_001640"
     CDS             complement(1113..1691)
                     /locus_tag="L3M60_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HBN6226317.1"
                     /translation="MNINEFLTHVNQGKPVSSKSEIHKFMVKLSNEAMRITSELNSYY
                     HEPEEIHSLFSELIGKKVDESFFMFPPFYTDCGKNITIGKNVFINSSCHFQDQGGIEI
                     GDNTQIGHNVVLATLNHGIAPEKRGTTYPSPITIGKNVWIGANVTVVPGVTIGDNAII
                     AAGAVVTKDVAENTIVGGVPAKILRVIDKITE"
     gene            1917..2855
                     /locus_tag="L3M60_001641"
     CDS             1917..2855
                     /locus_tag="L3M60_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6226318.1"
                     /translation="MEIRVLRYFLAIAREESISGAATFLHITQPTLSRQIIELEEELG
                     VKLLVRGNRKITLTNEGKLLHKRAEEIISLVDKVELEFDNPNEIISGDIYIGGGETEA
                     MRLIARTAHNFHQKYPNVKYHLFSGNADDVTEKLDKGLLDFGILIEPANIQKYDYTQI
                     PATDVWGLLMRKDSTLASQNTINPKDLCNIPLIISRQTLVDKAISKWLQDSTEKLNIV
                     ATYNLVYNASLLVEEGLGYALCLDRLVNTTENSNLCFRPLSPPLEAKLNIIWKKHQIF
                     SKAAEKFLLQIQKDFSSILLDTSELDISKLNTPEVK"
     gene            complement(3029..4171)
                     /locus_tag="L3M60_001642"
     CDS             complement(3029..4171)
                     /locus_tag="L3M60_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBN6226319.1"
                     /translation="MDFKIEKYLLRKAEELAFITIKHGGEFKLKSYEVPKGGLDVPIK
                     NEVLVKGIKEKTAQDKLNSMSIADAMIYIIGIDSKFKNNAEYEKFLNALSKDIDLDLK
                     AYMGYMSRKYFEIGEHTDSLIYIKAFITMYPDDLDAMYNYAIVCQEIAKQYQKDMDDK
                     AMNAFLLEAMAKLEEVIDVDENFALGYYHLGYHYYNQGQYLKTKLTWEEALRLGLDAD
                     LVAEIQENLGKMDFKVQYEEGYTLVFQGKFKEGLEKLLPLEEEHMDWWNLLFMIALGY
                     KGMGEIEQAKMYLEKILIIKPNQVDTIVELGLCEAYKNNLDKAIEYFEQAAKIKEDPE
                     ILCNLGMAYLNNGDIDDATYYIERAYELNPQDEITIACLRELGINK"
     gene            complement(4189..5943)
                     /locus_tag="L3M60_001643"
     CDS             complement(4189..5943)
                     /locus_tag="L3M60_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HBN6226320.1"
                     /translation="MKILLTTLNSKFIHTNLAIRYLKEFVRDLIEVDMKEYTINNDLD
                     YILKDIYKNEYDIILFSTYIWNVGDIVKLCDNLKKIRPNTKIALGGPEVSYDSYDAMK
                     KYDFVDYILYGEGELIFRDLVLHLQGKMEINDVNGLVYRQGNEIIVNKPMELLQNLDE
                     IPSPYENLNPREYENRIVYYESSRGCPFNCQYCLSSTIPGLRYFSLERIKRDLKALID
                     ARVSQIKFIDRTFNANRKVAMEIMDFLMKNDNGYTTYHFEVTAYLIDDKMLEFLADCK
                     EGLFQFEIGVQSTNEKTLDAVGRRDDFKKLSHVVQTVASYRNIHQHLDLIAGLPYEDY
                     KSFENSFNDVFNLGIEHLQLGFLKMIKGTGMRKVADEHGFKYKDYAPYEFLYNNYISY
                     EETLKLKDIEDILERYYNSKNFVLSMRYIIGRFYKQSPFKFFEVFAKYFDENGYFDLA
                     QGKNQLYKILMDFYNEKINIDNDVFNDILKYDYISLGKTSNIPQFFNKLDVDDFKNRC
                     HVFLQDNDNLSTYLPSFVDKPAKHIIKYVHFEPFRFNVVDLKNDINTEIREAENVVLF
                     EYDDKKIFEKSKTHKVEI"
     gene            6079..6285
                     /locus_tag="L3M60_001644"
     CDS             6079..6285
                     /locus_tag="L3M60_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226321.1"
                     /translation="MELIEKVSINSLSKRDLLLIIKSLEYTNENTQISDFIDLRNNIV
                     KELCFLTDTKEKDFLDYLEKNSTL"
     gene            complement(6290..7213)
                     /locus_tag="L3M60_001645"
     CDS             complement(6290..7213)
                     /locus_tag="L3M60_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226322.1"
                     /translation="MNDELLKKVYTSSFSSASRALKDLIIINTKSLIDDKTQRCVYID
                     ENRLRDELIYYRFYGEKIGNINFLNILLPLILSNTNIQKSEDEVIKLIKKYVTYFKKE
                     KLLFEYLLSSVVYNSIMHNLINNSKIEYVELLQSIKDKIIGFSIELDKSDVVKFQMAR
                     IKAIQLIDKYIDLKFEDYDEESILLNILNVLYDVYMEDRTVEDEGINSIKKSILSILG
                     EDSKLNEDNIDFIFSMSEYVVKLRKYKIGVKAYNKKIDPRSLIRLEEGNTIIDPIFNQ
                     ITVISKTFNDNILSIKINSKSGIYILKFKKV"
     gene            complement(7467..9167)
                     /locus_tag="L3M60_001646"
     CDS             complement(7467..9167)
                     /locus_tag="L3M60_001646"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBN6226323.1"
                     /translation="MQDFKVAISNCLKEKIEDLSKEEIEALIEVPPNKDMGDYAFPCF
                     KLAKVFRKAPNMIASELAESIEPSGEITKVIQLGGYVNFFVNKSQLAETVIKKVLDEK
                     ENYGHSDFGKDKTVIVEYSSPNIAKPFHIGHIRTTVIGNALYKIYDSQGYKTIRINHL
                     GDYGTQFGKLIVAFKKWGEKEVVESNPIPELLKLYVRFHDEAEQHPEMEDEARAWFAK
                     LENGDEEAKELWQWFRDESLKEFNRVYKLLDIEFDSLAGESFYSDKMNRVIELLEEKH
                     LLKESKGARIVDLEEYKMPPALITKNDGSTLYMTRDLAAAIYRKETYDFDKCIYVVGS
                     QQNLHFQQWFKVIELMGYDWAKDLIHVGFGMVALEEGTMSTRKGRVVFLEDALNQAID
                     KTKEIILAKNPNAKNVDEISKQVGVGAVVFQELSNSRIKDYTFSWERTLSFDGETGPY
                     VQYTHARCCAVLRKAEVEVTSDIDYSLLADEDSAEVLRVIESFNKNILLALKKNEPHI
                     VTRFMLDLAQAFNKFYHDNPILVENLEIRKARLALVLATKQTLENSLRLLGMHAPERM
                     "
     gene            complement(9493..9906)
                     /locus_tag="L3M60_001647"
     CDS             complement(9493..9906)
                     /locus_tag="L3M60_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HBN6226324.1"
                     /translation="MILVSACLIGINCKYSGDNNENEKVKEYLKGKEFTLVCPEQLGG
                     LSTPRPPAEIVDGKVITKDNKDVTENFVRGANETLKIANLYVCSEAILKEGSPSCGCN
                     LIYDGSFSGKKIPGKGITARLLEEEGINVKSEKDL"
     gene            complement(9974..12352)
                     /locus_tag="L3M60_001648"
     CDS             complement(9974..12352)
                     /locus_tag="L3M60_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBN6226325.1"
                     /translation="MNEKSLRVLEYNKIIDLLKKKASSSLGLKYIENLVPNTDFVEVK
                     SMLEETSEAQSIIIKRGSVGLEGIHDIEDKVKRAYIGASLDPGSLIMIADTLRVARRL
                     RNSLSSSDEEDFNYPIIQSLSNSLYVYKDIEDQIYNAIISEVEISDNASSTLRDIRRR
                     IAQKNQSIRSKLNSIISSTTYQKYLQDAIISLRGDRFVVPVKAEYRSQVAGIVHDQSS
                     SGATLFIEPMTIVEMNNELRQLKLGEQEEIERILSELSAMVGEVSEDLISNQEILGRL
                     DFAFSKGKLSIQMRGIEPTLNEDKYLNIKNGRHPLLDKKKVVANTIYLGRDFHTLVIT
                     GPNTGGKTVTIKTVGLFALMTQSGLHIPADYGSSMCVYDNVFADIGDEQSIEQSLSTF
                     SSHMTNIVSILQNVTTDSLVIFDELGAGTDPVEGAALAIAVLEDINSVGAKCIATTHY
                     SELKNYALTKSGVENAAVEFDIETLSPTYKLLIGVPGKSNAFEISRKLGLSDYVISRA
                     KEYINTENIALEDVLQNVEKNRIKAVEDREEAERLKEEIERLKVEYDEKLEKLVSQRD
                     KMIEKAKSEAFSIIRQAKEEVDIIIKELRSLEQERASKEKNRKIEELRKELTSSMGSL
                     QPTVKSMIVPKVSNKEIKDLKPGEEVKVITLNQNGSVVSVDKKRKEAVVQIGIMKMTL
                     PFKSLQKTHKDVSTNVTKSTRNIIRSKSGSVKNEVDLRGLNLEEAIMEVEKYLDDAYV
                     AGLESVTVIHGIGTGVLKAGLQDILRRNRHVKSQRGGQYGEGGAGVTIVKLK"
     gene            12669..14120
                     /locus_tag="L3M60_001649"
     CDS             12669..14120
                     /locus_tag="L3M60_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HBN6226326.1"
                     /translation="MGNVLEGLKPESVFKNFEKISQIPRGSGNEKGISDFLLSFGKNL
                     GLETIQDESLNIIIRKPATKGYENCPGVVLQGHMDMVCEKEKNVEHDFLKDPIKLRID
                     GDMIYATGTTLGADNGIAVAMGMAILEDNTLEHPALEVLITVNEEDGMNGADALDPSL
                     IKGQYILNLDSEEEGYLLVSCAGGKTCVVSLPVEYKKVEGDKQGLLVEVTGLLGGHSG
                     MEIVLQRANANKAIARVLSVLDVDYELASVDGGTKHNAIPREAKCVIAVNKADVESAK
                     KQINDILTAFKHEFTTSDPGMTYSVAETSVDKVLTKDCKEKVVQMSCLTPHGVQSVSL
                     DIEGLVESSTNFAIIETKESTIEFLTSVRSSVMSIRDEIADRIRLLAQALGANYDLIA
                     QYPAWEFKKDSKLEKICCETYERLTGKVPTVMALHAGLECGLLLDKLPHAEAISIGPD
                     MFDVHTPNEHVSIPSVANIWDYVIEILKSMNQY"
     gene            complement(14324..16840)
                     /locus_tag="L3M60_001650"
     CDS             complement(14324..16840)
                     /locus_tag="L3M60_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBN6226327.1"
                     /translation="MEDCVVNLKKLLLLIIVITSIFTSYIFSFSINAIANDNRTIRIA
                     YPIQKGLTEIDEQGNYYGYTYEYLLEIAQYTGWNYEFVQVPGDINESITKLMKMLENG
                     EIDIMGGTLYNESLQKIYNYAGNSYGVANTVLQALYENTDIDNIMSSQGVKKIRIAVI
                     ENSDTRLKELDEYCKMNLMSPELVYCKTNEEQLEALKNGKADVLLNVSMNPIEDVRTV
                     AKFSSRPFYFITPKGSTDLTQRLNSAINNIEQKDPYFSTTLYEKYFTPENNQMFLSDE
                     ETNYIKDTEKLKVGVLLNKPPFQNIDKNTGELKGISIDLLNHICDLTGLEFELVPIKS
                     QKDLEKMTKNHEIEIVAGIIYDYEYSRKHNIIMSRPFVSTQYAMMINNKLNENSLDGK
                     CLALTRGLNYEGQFKENIVWYDTLEECIEAVNKGDVDYTYGEGYSMQYYMNQPKYKNV
                     RLVPQTYEAQSICFGIIKKDKQELLNIFNKAITSISSEKMQSIVYQNTTYRQDFSLLY
                     IMQQNPIETIFIVASLLICIIGFMAFGLHTRTSMNEKISLELKKHTQLYEMTSEYFFE
                     YDYKQDKLIISMPEKGIETYYGLDKNDRESENNNNIANEIDKIRREYNKKFFDVIKSQ
                     ENGTTEMYSIVFDDTFHWIRITSKIIYNDRKIPMYVIGKISNIDYEKEEKNRLLDKVQ
                     RDSLTNIYNAAYIRKLTAQNLLNLSTEKNGALLIIDIDYFKSINDTYGHLVGDKILID
                     VAKVLDESFTKDDIVGRLGGDEFSVYINDIINNEELINKCNDVYNRIHLIKLPNGKSL
                     SISMGVVIAKCGQEYDELYQIADNALYDSKREGRNRFKIV"
     gene            complement(17062..17883)
                     /locus_tag="L3M60_001651"
     CDS             complement(17062..17883)
                     /locus_tag="L3M60_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6226328.1"
                     /translation="MIDINLEKCVGCGMCESDCLVNAIKVKDDKAKVKNILCINCGHC
                     MAICPTDAIEMQGFDKNEVVEYNRESFELEPEKLLNFIKFRRSIRQYKDIEIEEEKIK
                     NIVEAGRYTPTGGNRQPIRYILVKEKLKEVKELAIEGLYNLALNTDDNDPVRSMYKNT
                     FKKMYKRYKENGKDSLFYDAPLLMVVVGDMSLGGSAYVDGGLAASNMELMAYSQGLGI
                     CYNGFFVMASNVEPKIKELLGMSENEAVITSFILGYPDVKYKRTVNRNTAKFEVI"
     gene            complement(18417..18893)
                     /locus_tag="L3M60_001652"
     CDS             complement(18417..18893)
                     /locus_tag="L3M60_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HBN6226329.1"
                     /translation="MKLTTICYIEKDDKTLMLYRNKKKDDIHEGKYVGVGGKFEQGET
                     PEECVIREVKEETGLTLKSLSYKGLITFPKFKDEEDWYMFLYFSDEFEGELSEKDLND
                     CKEGNLIWVDNDKIFDLNMWEGDRLFLNWAKTGNIFSAKIVYNNGKLKDYNVSFLD"
     gene            complement(18927..19505)
                     /locus_tag="L3M60_001653"
     CDS             complement(18927..19505)
                     /locus_tag="L3M60_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HBN6226330.1"
                     /translation="MREEALEILFKYLETDRMRKHCYAVEAVMRDLAKRLEPEKEEEW
                     AVAGLLHDLDSDIVGRKPGEICEGHATTTVELLKKENFGDEEMYRAILGHHDGMGVTR
                     TSLMEKAIYAADPITGFITAIALVYPDKKLTSVKTKSVIKRMKETRFASNVNRDAMRS
                     IEDIGIPFDEFAELSLNAMKNISDVLESAENN"
     gene            complement(19609..22164)
                     /locus_tag="L3M60_001654"
     CDS             complement(19609..22164)
                     /locus_tag="L3M60_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBN6226331.1"
                     /translation="MKKIELLAPVGSFDSLKAAVQNGANAVYLGGKDFSARASANNFD
                     RNELIEAVKYSHIRGVQVFVTVNTLIKQYEIEDFVEYVKFLYDIDIDALIVQDIGMAR
                     LIKKLLPDFELHASTQMVAHSLEDVKYLQSVGFDRVVLARELDVDEIKWICENTTVDI
                     EVFVHGALCVCYSGQCLMSSMIGNRSGNRGRCAQPCRQKYELIDINTGEVVKSNGDYL
                     LSPRDLSTIEDLDKIIEAGVLSLKIEGRMKKPEYVATVVSGYREAINEFVDKKKISIS
                     EEIINNLYTIFNRKFTKGYILGEVGKDIMNSNRPNNQGLYIGKVLDYNRKNKRLRVML
                     ENTLKKGDGINLGGGTIGRIIKDGEITTIAHKGDLIELDFVGEAKRNQLIYKTSDSEL
                     MDRVQATFQQDKEFIKTNISAKVSIKLGKKPELFISDFCGNKSVVEGDKIVEKAIKVA
                     LSKEKIETQIQKLGNTPYKLKEIEIDLDEGVSMPISVLNQMRRDCIELLDGERIKIKD
                     RKFKKNILKYQPNRVNKQKKLNTVPKIRVKVKNLEQLEAVLNYDVDLIYYEDINTLSK
                     ALQMVSSISIDSSISTDLDTSSSSSISKELMGSNPNNIDTLNNKRSNTAVTDKKLIYS
                     APRIVRNKEYKQFEILDNIKGINNVQIGNWGCMEYFKDKDLYIDCYLNAFNSESINHF
                     KEEGASTVCLSQELNLNEIKQTLKYTDVETEAVIYGYAPMMVTEYCPMGVVVRDCKKD
                     KRVAKCKESNYALRDFTDASYRITQDVFCRSTIYNSKVTCMLDNLCELDEIGIDVFRI
                     DFTVEDSEMVKKVIEAHIEVLSNNYKLGEKATDLYKKLDEIGTVAGHYYKGVE"
BASE COUNT         7072 a         4048 c         2506 g         8886 t
ORIGIN      
        1 ttatataaaa tttttttaat acaatattaa tttagacatt tttactattt tttctacttt
       61 aatttattta tttcaaaata tttatcctat ttcatatttt tagctacaat agcaccatat
      121 gcaatagaat ataatttaga ttcatgtgaa tcagctcttg acactaaaac tataggagct
      181 tttgcaccca taattatacc tgctgattta gagtctgcca tgtatgtaaa ggccttccct
      241 attccattac caacctctat tgttggaacc aatatgatat ctacatctcc agatacttca
      301 cttttaaacc cttttatctc actagcttct tttgaaacag ccaaatcaaa agccagagga
      361 ccttctacaa ttacattgtc accaaattct ccttctttgc aagcctttgc taacatatct
      421 gcatctacag ttgcttgcat cttaggattt actttctctt tagctgccaa acacgcaact
      481 tttacagttt ccattcctaa agcttttgca gcttttatag aattttttaa tatctttacc
      541 ttttgttcat aatcaggagc tatattcata cctccatctg tagttataag taatttatgg
      601 tatttttcta actcatatac cataacatga cttaataaac tatcagttct aagaccatag
      661 tctttattta aaacttcttt taatatgaca gatgtatcta ataatccttt cattacaaag
      721 tcggcttctt tacttgatac taatttaact gctatttctg cacactcttt gatatcatcc
      781 ttatccacta ttttgatacc acttaaatca aatcctattt cttttgatat ttctcttatt
      841 ttgccttcat ttccaaccaa tataggagta attatatttc tttctacagc atcttttatt
      901 gccattaaaa cttctttatc atgagctgct gctacagaga gtacaatttt ctctcctcct
      961 ttaagttggt ctaaaatatc atctaatttt tttatcaaaa gtaacccctc ccttatatat
     1021 atttaactaa ataataccat atttaatata ccttttctca atttattttt atgttataaa
     1081 gaatttatat aactaaaaga ccgtttaata ttttattctg tgattttatc aataactctt
     1141 aaaatctttg cagggacacc acctacaata gtattttctg caacatcttt tgtgacaact
     1201 gctccagcag ctataattgc attatctcct atagtaacgc ctggaactac agttacatta
     1261 gcaccaatcc atacattttt tcctattgtg attggagaag gataagttgt accacgtttt
     1321 tctggtgcaa tgccatggtt aagtgtagcc aataccacat tatgcccaat ttgagtgtta
     1381 tctccaatct caataccacc ctggtcttga aaatggcaac tgctatttat aaaaacattt
     1441 tttcctattg taatgttttt tccacaatct gtataaaatg gtggaaacat aaaaaatgat
     1501 tcatctactt tctttccaat taactcagaa aataaactat ggatttcttc tggttcatga
     1561 taataagagt ttaactcact tgtgattctc atagcttcat tactcaactt aaccataaat
     1621 ttatgtattt ctgatttact agagacgggc tttccttgat tgacatgtgt taaaaattca
     1681 ttgatattca ttttatacat cccttcaata tgcgctttaa tttatttgta ccttctaaat
     1741 aatattttaa taataactaa tttaatttat tttttaggca atattattgc tccttttagt
     1801 gtaatatata attctatcta tatcaaatat ctatattaaa tacttaacta tacttaaaaa
     1861 gcataataat tgatataatc atataataaa caaattaata ttgaggagtg ataattatgg
     1921 aaataagagt attgagatat tttttagcaa ttgcaagaga agaaagtata tctggtgcag
     1981 caaccttttt acatataaca caaccaacac tttctagaca gattatagaa cttgaagaag
     2041 agttaggagt taaattattg gttagaggaa atagaaaaat caccttaaca aacgagggta
     2101 aacttttaca taaaagagca gaagaaatta tatcattagt tgataaagtt gaattagagt
     2161 ttgacaatcc caatgaaatt attagtggtg atatatatat aggaggtggt gaaactgaag
     2221 ctatgagact tatagctaga actgcacata attttcatca aaaatatccc aatgtaaaat
     2281 accacctttt tagtgggaat gctgatgatg taactgaaaa attggacaaa gggcttcttg
     2341 attttggtat tttaattgaa cctgcaaata tacagaagta tgattataca caaattcctg
     2401 ctactgatgt atggggttta ctaatgcgta aagatagtac cctcgcatca caaaatacga
     2461 taaatccaaa agatttatgc aatatacctc ttattatttc aagacagaca ctcgttgata
     2521 aagcaatttc taaatggctt caagacagta ctgaaaaact aaacattgtt gcaacataca
     2581 atttagttta taatgcttct cttttggtag aagaaggtct gggctatgct ctgtgtttag
     2641 atagactcgt caatactaca gaaaatagta acctttgttt tagacctcta tctcctcctt
     2701 tagaagctaa attaaatatt atatggaaaa aacatcaaat attttcaaaa gcagcagaaa
     2761 aatttttact tcagatacaa aaagattttt cttctatcct gttggataca tctgagttgg
     2821 atatatctaa gttgaataca cctgaagtaa aataaataat aataaaaaat taaggtgtct
     2881 caaaatgaac tttttacttc ataatggctc tttgttgtat gaagtcaaat atatgttaat
     2941 catttcagca ataaaaagag gcttatctct attttgagac agcctcattt aaccaataaa
     3001 atcaaataca acctaacaat atccttattt atttatttat tccaagctct cttaaacaag
     3061 caatcgtaat ttcatcttgt ggatttaatt cataagctct ttctatatag taagttgcat
     3121 catcaatatc accattattt aaataagcca ttcctaaatt gcaaagtatt tctgggtctt
     3181 cctttatctt agctgcttgt tcaaaatact ctattgcttt atctagatta ttcttataag
     3241 cctcacaaag ccctaattcc actatagtat ccacctgatt aggctttatt attaatatct
     3301 tctcaagata catcttagcc tgttctattt cgcccatacc cttatatcca agtgctatca
     3361 tgaacagcaa gttccaccaa tccatatgtt cttcttctaa tggaagtaac ttttctaatc
     3421 cttctttaaa cttaccttgg aatacaagtg tatatccttc ttcatattga actttaaaat
     3481 ccatcttgcc taaattctct tgtatctcag ctaccaaatc agcatctagc cctagtctta
     3541 acgcctcttc ccaagtcaac ttagtcttta aatattgacc ttgattataa taatggtagc
     3601 ctaagtgata ataacctaat gcaaagttct catcaacatc aattacctct tcaagcttag
     3661 ccattgcttc aagtaagaat gcattcattg ccttatcatc catatctttt tgatactgtt
     3721 ttgctatctc ttgacaaaca atcgcataat tatacattgc atctaaatca tctggataca
     3781 ttgttataaa tgcttttata tatattaaag aatctgtatg ttcaccgatt tcaaagtatt
     3841 ttctcgacat atatcccata tacgctttca aatccaaatc aatatctttt gataaagcat
     3901 ttaaaaactt ctcatactct gcattgtttt taaacttact atctatccca attatatata
     3961 tcattgcatc tgctatagac attgaattta acttatcttg agcagtcttt tcttttatgc
     4021 cttttactaa tacctcgttt tttataggta catctaatcc accttttggt acttcatagc
     4081 tttttaattt aaactctcca ccatgtttta tagttataaa agctaattcc tctgcttttc
     4141 ttagcaaata tttttctatc ttaaaatcca ttttatcctc ccaaatactt aaatctctac
     4201 tttatgagtt ttagatttct caaatatttt tttatcatca tactcaaata agactacatt
     4261 ttctgcttct cttatttctg tatttatatc attttttaaa tccacaacat taaatctaaa
     4321 tggctcaaaa tgaacatatt ttattatgtg cttagctggt ttatcaacaa aactaggtaa
     4381 atatgttgat aagttatcat tatcttgtaa aaatacatgg catctattct taaaatcatc
     4441 cacatctagc ttattaaaaa actgtggtat atttgatgtt ttgcccagtg atatatagtc
     4501 atattttaat atatcattga atacatcatt atctatattg attttttcat tatagaaatc
     4561 catcaatatt ttatataatt gattctttcc ttgtgccaaa tcaaagtagc cattttcatc
     4621 aaagtactta gcaaaaactt caaagaattt aaaaggactt tgtttataaa atctgcctat
     4681 aatatatctc atagataaca caaaattttt agaattgtag tatctctcca gtatatcttc
     4741 tatatccttt agcttcaaag tttcttcata gcttatataa ttattgtata aaaactcata
     4801 tggagcgtaa tctttatatt tgaaaccatg ctcatctgca acttttctca tacctgtccc
     4861 tttaatcatc tttaaaaatc ctaattgtag atgttctata cctagattaa atacatcatt
     4921 aaaagaattt tcaaagctct tataatcttc ataaggcagt cctgcgatta agtctaagtg
     4981 ctgatgtata tttctataag atgctacagt ctgtacaaca tgggacaact ttttaaaatc
     5041 atctcttctt cctactgcat caagtgtttt ttcattcgtt gactgaacac ctatttcaaa
     5101 ttgaaaaaga ccttctttgc aatcagccaa aaattcaagc atcttatcat ctatcaaata
     5161 cgctgtaact tcaaaatgat aagttgtata tccattatca tttttcataa gaaaatccat
     5221 aatttccata gccacttttc tatttgcatt gaatgtccta tcaataaact ttatctgtga
     5281 tactcttgca tctattaagg cttttaaatc tctctttatc ctctctaaac taaaatacct
     5341 tagacctgga atagtagatg ataaacagta ctggcagtta aaagggcaac ctcttgatga
     5401 ttcataatac actattctat tttcatattc tcttggattc aaattttcat aaggactagg
     5461 aatctcatcc aaattttgaa gtaattccat aggcttattc actattatct cattgccttg
     5521 tctatataca agtccattta catcatttat ttccatcttt ccttgcaaat gaagtactaa
     5581 gtctctaaaa attaattctc cctctccata aagtatataa tccacaaaat catacttttt
     5641 cattgcatca taactatcat aactaacttc tggtccgccc aaagctattt tagtattagg
     5701 tctaattttc tttagattat cgcaaagctt aactatgtct cccacattcc agatatatgt
     5761 tgaaaacaat attatatcat attcgttttt atatatatcc ttcaaaatat agtccaaatc
     5821 gttgtttatc gtgtattctt tcatatcaac ttctatcaaa tctcttacaa attcttttaa
     5881 atatcttata gccaaatttg tatgaataaa cttagaattt aatgttgtta ataaaatttt
     5941 caaatatttc tcacctcttt ttaagattat actgtctttt tgtacccctt ataggttaaa
     6001 atagtatatg taaagtctaa ataatatcta tataattaat ttattaattt caagtaatgt
     6061 gaggaaagga agttttttat ggaattaatt gaaaaagtat ctatcaactc tttatcaaaa
     6121 agagatttac tacttattat aaagtctctt gaatacacta atgaaaatac tcaaattagt
     6181 gatttcattg accttaggaa taatatagta aaggaattat gttttttaac tgacactaag
     6241 gaaaaagatt ttttagatta tctagaaaaa aatagcacac tctaatcacc taaacctttt
     6301 taaactttaa tatataaata cctgatttag aatttatttt tatgcttaat atattatcat
     6361 taaaggtttt tgatataact gttatttgat taaatatggg gtctattata gtattacctt
     6421 cttcaagtct tataagactt cttgggtcta tttttttatt gtatgcctta actccaattt
     6481 tatattttct caatttaact acatattcag acatagaaaa aataaaatct atattatctt
     6541 catttaattt actatcttca cctaggatgg ataatataga tttttttatg ctattaattc
     6601 cttcatcttc tacagttcta tcttccatat atacatcata tagaacattt aaaatattta
     6661 ataaaatact ttcttcatca taatcttcaa atttcaaatc aatatattta tcaattaatt
     6721 gtattgcttt aatccttgcc atctgaaact ttacaacatc tgatttgtct aattctatag
     6781 aaaatcctat tatcttgtct ttaatactct gtagtaattc gacatattct attttactat
     6841 tatttataag attgtgcata atggaattat acactacaga acttaataaa tactcaaata
     6901 aaagtttttc ttttttaaaa taagtaacat atttttttat tagtttaata acctcatctt
     6961 cgcttttttg aatattagta tttgataaaa ttagtggtaa taaaatattt aaaaaattga
     7021 tattgcctat tttttctcca taaaatctat aataaataag ttcatctctc agtctatttt
     7081 cgtctatata cacacatctt tgcgttttat catctattaa ggattttgta tttattataa
     7141 tcaaatcttt aagagcccta cttgcactac taaaagaaga tgtatagact ttttttagta
     7201 attcgtcatt cataaatata tcctttctga aaaattgaat gtaaataaat tgttattaaa
     7261 aaaaaagaga cagcccattt aagaagctgt catatatagg gggatagtag tgtacaaaaa
     7321 tcattgtaat ctactatcta tatattatta tacgatacta cttttttata tgtaaagcat
     7381 cagttttcga catccctata ttttacagtt atacaaattg cgtactaagt tcaattttat
     7441 tctttttatt tcaacatttt gtcatgctac attctttccg gagcatgcat tcccaacaat
     7501 ctcagagaat tttctaaagt ttgctttgtt gctaaaacta acgcaagtct tgcttttctt
     7561 atttccaaat tttctactaa aattggattg tcatgataaa atttattaaa cgcttgagct
     7621 aaatctagca taaatctagt cactatatgt ggctcatttt tctttaaagc aagtaaaata
     7681 ttcttattga atgattctat aactctaaga acttctgcac tatcttcatc agctaataga
     7741 ctataatcta tatcagaagt cacttctact tctgcttttc ttaaaactgc acagcatcta
     7801 gcatgtgtat actgaacata tggtccagtc tcaccatcaa aactaagcgt tctttcccaa
     7861 gagaaagtat agtctttaat tctactatta gataactctt ggaatactac tgccccaaca
     7921 cccacttgct tagatatctc atctacgttt ttggcatttg ggttttttgc tagtataatc
     7981 tccttagtct tatctatagc ttgatttaaa gcatcttcta aaaatacgac tcttcctttt
     8041 ctagttgaca tagttccttc ttcaagagct accataccaa atccaacatg aattaagtct
     8101 tttgcccaat catatcccat aagctcgatt accttaaacc attgttggaa atgtaagttt
     8161 tgttgagaac caactacata tatacacttg tcaaaatcat aagtttcttt tctatatatt
     8221 gcagcagcta aatctcttgt catatataat gttgaaccat cattcttagt aataagtgct
     8281 ggtggcattt tatactcttc taaatcaaca attcttgctc ccttagactc ttttaaaaga
     8341 tgtttctctt ccagtagttc aataaccctg ttcattttat cagaatagaa actctcacct
     8401 gctaaagaat caaattctat atctaaaagt ttatacactc tgttaaactc ttttaaactc
     8461 tcatctctaa accattgcca taattccttt gcttcttcat caccattttc taatttagca
     8521 aaccatgctc ttgcttcatc ttccatttct ggatgttgtt cagcttcatc atggaatctt
     8581 acatataatt ttagtaattc tggaattgga ttagactcta ctacttcttt ttctccccac
     8641 tttttaaagg ccactattaa cttaccaaac tgagttccat agtcacctaa atgatttatt
     8701 cttatagtct tatatccttg tgagtcatat atcttatata aagcattacc tataactgta
     8761 gttcttatat gccctatatg gaaaggtttt gctatatttg gagaagaata ttcaactatt
     8821 acagttttgt cttttccaaa atcactatgc ccgtaatttt ctttttcatc taaaactttt
     8881 ttaattacag tttctgctaa ttgagattta tttacaaaga aatttacata tccgcctaat
     8941 tgtattactt ttgttatctc tccacttggt tctatacttt ccgccaattc acttgctatc
     9001 atgtttggag cttttctaaa cactttggct aatttaaagc atggaaaagc atagtccccc
     9061 atatccttat ttggtggaac ttctataagt gcctctatct cttctttcga taaatcttct
     9121 attttttctt taaggcaatt tgatattgcc accttgaaat cttgcattct gttgctcctc
     9181 tctacattgt tcctggttta tattgtgctt atctctttat gttaaatcaa atttcgtgtt
     9241 ttttcaagta tttcattaca gtgcaaaaca ctttaataat tatgttttct gtatatatta
     9301 aaaatactat aattacaatt tttaatcaca cgattattgg ttttttataa acttttttct
     9361 atcttattgt ttcttatact acccgtttga atttacatac ttttttactc ttttgccatt
     9421 tttcatacat caatgttgac tttatatact tttttactct tttatcattt tttatgcatc
     9481 agtgttaatt tgctataagt ctttttcact cttaacgttt attccttctt cttccagtaa
     9541 tcttgccgtt atgcctttgc ctggtatctt tttacctgaa aaactaccat catatattag
     9601 attacatcca caagaggggc taccctcttt tagtattgcc tcgctacata catataaatt
     9661 tgctattttt agagtttcat tagcccctct tacaaagttt tctgttacat ccttattatc
     9721 tttagtaatt acttttccat caactatctc agcaggtggt cttggtgtag atagaccacc
     9781 taactgctct gggcatacaa gtgtaaattc tttgcctttt aaatattcct taactttttc
     9841 attttcatta ttatcgccac tatatttaca atttattcct attaaacatg cagataccaa
     9901 tatcataaaa atgtgctcct atacaaatta ttaatatatt ctcatacaaa ttactaatgt
     9961 attcttatac aaattatttt aatttaacaa ttgttactcc tgctccacct tctccatatt
    10021 gaccacctct ttgtgatttt acatgtctat ttcttcttaa aatgtcttgt agccctgctt
    10081 ttaatactcc tgtacctatt ccatgtatta ctgttacaga ctctaaacct gccacatatg
    10141 catcatcaag atacttttcg acttccatta tagcttcttc taggtttaat cctctaaggt
    10201 caacttcatt ttttacacta ccagattttg accttataat atttctagtt gatttggtta
    10261 catttgttga aacatctttg tgtgtttttt gtaaagactt aaaaggtaga gtcatcttca
    10321 ttataccaat ttgcactact gcttcttttc tctttttatc tactgaaact acactaccat
    10381 tttgatttaa agttattacc ttaacttctt cacctggctt taagtctttt atctccttat
    10441 ttgatacctt tggaactatc atacttttta cggttggctg aagacttccc atagaactag
    10501 tcaattcttt tctaagttct tctatcttac gattcttttc ttttgaagct cgttcttgtt
    10561 caagacttct aagttctttt attataatgt ctacttcttc tttagcttgt cttataattg
    10621 aaaatgcttc tgatttagct ttttcaatca tcttatctct ttgactaacc aatttttcaa
    10681 gtttttcatc atactctact ttcagtcttt caatctcttc ttttaatctt tcagcttctt
    10741 ctctatcttc tactgctttg attctatttt tttcaacatt ctgtaacaca tcttctaaag
    10801 ctatgttttc tgtatttata tattctttag cacgtgaaat cacataatca cttagcccaa
    10861 gttttctaga aatttcaaat gcatttgatt ttccgggaac acctataagt aatttatatg
    10921 tcgggcttaa agtttctata tcaaattcaa ctgctgcgtt ttctacacct gatttagtaa
    10981 gagcataatt tttaagctca ctatagtgag tggtagctat acatttagct cctacagaat
    11041 ttatatcttc taatactgct atcgcaaggg cagctccttc aacagggtct gttcctgctc
    11101 ctaactcatc aaatataact aaggagtctg ttgttacgtt ttgtaatatt gatactatat
    11161 tcgtcatatg agatgaaaat gttgatagac tctgttcaat actctgttca tctcctatat
    11221 ctgcaaatac attatcatat acacacatac tacttccata atcagcaggt atatgaagtc
    11281 cactttgtgt cataagtgca aatagaccta cagtttttat tgtaacagtc ttaccacctg
    11341 tgtttggacc tgttatcacc aatgtgtgga aatctcttcc taaataaatc gtatttgcta
    11401 ctactttctt tttatccaac agtggatgtc ttccattttt tatgtttaaa tatttatcct
    11461 catttaaagt tggttctatt cctctcatct gtatagaaag ttttcccttt gaaaatgcaa
    11521 agtccaatct tccaagtatc tcttgatttg agattaaatc ttcactcact tctccaacca
    11581 tagcagaaag ctctgataaa attctttcta tttcttcttg ctctcctagt ttcaactgtc
    11641 tcaattcatt gttcatctct actatagtca ttggctctat aaaaagtgtc gcaccagaag
    11701 atgactggtc atgcactata ccagctactt gcgacctata ttcagctttt acaggtacta
    11761 caaatctatc acctcttaga gaaattattg catcttgtaa gtatttttga taagtagttg
    11821 aacttattat tgaatttagc tttgacctaa tggattgatt tttttgagct attcttcttc
    11881 tgatatctct aagtgtactt gaagcattgt cagatatttc tacttcacta attatagcat
    11941 tgtatatctg gtcttctatg tccttatata catatagaga atttgataga gattgtatta
    12001 ttggataatt aaaatcttct tcatcactgc tagataagct atttcttaat cttctagcca
    12061 ctcttaaagt atctgctatc ataatcagac ttcctgggtc aagagaagca cctatataag
    12121 ctctctttac cttgtcttct atatcatgaa taccttccaa gcctactgaa cccctcttta
    12181 taataattga ttgagcttca ctagtttctt ctagcataga tttcacttct acaaaatcag
    12241 tatttggtac taaattttct atatatttta gacctaagga agatgaggct ttcttcttta
    12301 acaaatctat tattttattg tattctagaa ctcttaacga tttctcattc attttctata
    12361 ttcaccatcc tcgatatttt aactttttta tttaatttta aaaatgtatg ttttcaagca
    12421 tttccaataa aaactgcaag ttttatttct ttttcatttc taaaattatt atattttttc
    12481 tcttttagat atattttaca catattttgt attatactat gtatgtagct atctatatat
    12541 tatacataaa catgctttat ttgtaatata atctgttaaa aaatcttctt taatattttt
    12601 aaaggagttt attttagctt aaaaaaaaat agctacttat atagataaat taaggaggaa
    12661 catgagtcat gggaaacgta ttagaaggat taaaaccaga aagtgttttt aaaaactttg
    12721 agaaaatatc tcaaattcct agaggttctg gaaatgaaaa gggcataagt gattttttac
    12781 ttagctttgg taaaaattta ggtcttgaaa ctattcaaga tgaaagctta aacataataa
    12841 taagaaagcc tgctacaaaa ggatatgaaa attgtccagg tgtagttctt caaggacata
    12901 tggatatggt ttgcgaaaaa gaaaagaatg ttgaacatga ttttcttaaa gaccctataa
    12961 aattaagaat tgatggtgac atgatatatg ctactggaac tactctagga gctgacaatg
    13021 gtatagctgt tgctatgggt atggctatat tagaagataa tactttagag catcctgcac
    13081 tagaagtttt aattactgtt aatgaagaag atggtatgaa tggtgctgat gcattagacc
    13141 ctagcttaat taaaggtcaa tatatcctaa atctggactc cgaagaggaa ggatacttat
    13201 tagttagctg tgctggtggt aaaacttgtg ttgttagttt acctgttgaa tacaaaaagg
    13261 ttgaaggtga caaacaagga ttattagttg aagttactgg attattaggt ggacactctg
    13321 gtatggaaat tgttttacaa agagcaaatg ctaacaaagc aatagctaga gtattaagtg
    13381 tattagatgt tgattatgaa ttagcttctg ttgatggtgg tactaagcac aatgctatac
    13441 ctcgtgaagc taagtgtgta atagctgtta ataaagctga tgttgaatct gctaaaaaac
    13501 aaataaatga cattttaact gcatttaaac atgagttcac tacttctgac cctggcatga
    13561 cttactcagt agctgaaact tctgtagaca aagttcttac taaagattgt aaagaaaagg
    13621 ttgttcaaat gtcttgctta actcctcatg gagttcaatc agtaagtctt gatatagaag
    13681 gattagttga aagttctact aacttcgcta taattgaaac taaagaatct actattgaat
    13741 ttttaacttc tgttagaagt tctgttatgt ctattagaga tgaaattgct gatagaataa
    13801 gacttttagc tcaagcttta ggtgctaatt acgaccttat agctcaatac ccagcttggg
    13861 aatttaaaaa agactcaaaa ttagaaaaaa tatgctgtga aacttatgag agattaactg
    13921 gtaaagttcc tactgttatg gctctacatg ctggtcttga gtgtggatta ttacttgata
    13981 aacttcctca tgctgaagct atctctatag gaccagatat gtttgatgtt catactccaa
    14041 atgaacatgt tagtatccct tctgtagcta atatttggga ttatgttatt gaaatattaa
    14101 aatctatgaa ccaatattaa atttaagttt tgattctaaa tagattttag tcttaagtaa
    14161 tctatataaa attaactctg taagttaata ctactgttag tataacattt actttcaata
    14221 ttaacttata gaaatagagc ctaatgatag atagtaacat gtatcattag gctttattgt
    14281 aatttaaata ttattaagct ttattctgtt ttaaaattaa ttcttatact attttaaatc
    14341 tatttcttcc ttctctctta gaatcgtaaa gagcattatc tgctatttga tacagttcat
    14401 catactcttg accacacttt gctataacta cccccatgct aatagacaaa ctttttccat
    14461 ttggcaattt aatgagatga atacgattgt atacatcatt acacttattg ataagttctt
    14521 cattatttat tatatcattg atataaacac taaattcatc tccacctaaa cgacctacta
    14581 tatcatcttt tgtaaaactt tcatcaagta cctttgcaac gtcaatcagt attttatctc
    14641 ctaccaggtg accataagta tcattaattg acttaaaata atcaatatct ataatcaata
    14701 aagcaccatt tttttcagtt gataaattca ataaattttg tgcagttagt tttctaatat
    14761 atgctgcatt ataaatattg gttaaactat ccctttgaac tttatctaat aatctattct
    14821 tttcctcttt ttcataatca atattagata ttttcccgat tacatacatt ggtatttttc
    14881 tatcattata aattattttt gaagtaattc taatccaatg aaaagtatca tcaaacacaa
    14941 tagaatacat ttcagtcgtt ccattttctt gtgattttat tacatcaaaa aactttttat
    15001 tatattctct tcttatttta tctatttcat ttgctatatt gttgttattt tcactttccc
    15061 tatcattttt atctaaacca taataagtct caataccctt ttctggcata gaaattatca
    15121 atttatcttg cttataatca tactcaaaaa aatattcact tgtcatttca tacaactgag
    15181 tatgtttttt gagttccaaa gatatcttct cgttcatact cgtacgagta tgtaaaccaa
    15241 atgccataaa tccaataata caaattaaca aactagctac aataaaaata gtctcaattg
    15301 gattctgttg cataatataa agtaatgaaa aatcttgtct atatgtagta ttttggtaga
    15361 caattgattg cattttttct gatgaaatac ttgtaatagc tttattaaat atattcagta
    15421 attcttgttt atctttttta attatcccaa aacaaatact ctgtgcttca taagtttgag
    15481 gaactagacg cacattttta tacttaggtt gattcatata gtactgcata gaataacctt
    15541 caccataagt ataatctaca tctcctttat taacagcttc tatacactct tctaaagtat
    15601 cataccacac aatattctct ttaaactgac cctcataatt aagtcctcta gttagagcta
    15661 gacattttcc atcaagacta ttctcattta atttgttatt aatcatcata gcatattgag
    15721 tagaaacaaa tggtcgagac ataattatat tatgtttacg agaatattca taatcataaa
    15781 ttattcctgc tacaatctca atttcatgat ttttggtcat tttttccaaa tctttttgag
    15841 attttatagg tacaagttca aattctagcc ctgttaaatc gcatatgtga tttaacaaat
    15901 caattgatat accttttaac tcacctgtat ttttatctat attttgaaat ggtggtttat
    15961 ttaaaagtac tcctaccttt aacttttctg tatctttaat gtagttagtt tcttcatcag
    16021 ataaaaacat ctgattattc tctggtgtaa aatatttctc atataaagta gttgaaaaat
    16081 atggatcctt ctgttcaata ttattaattg ctgaattgag tctttgagtt aagtctgtac
    16141 tacctttagg agtaatgaaa taaaacggtc ttgatgagaa ttttgctaca gtacgtacat
    16201 cttctattgg attcatactc acattaagaa gtacatctgc tttaccattc tttagtgctt
    16261 ctaactgttc ttcatttgtt ttacagtata ccaattcagg tgacatcaaa ttcatcttac
    16321 aatactcatc taactcctta agcctagtat cagaattttc tattacagca atacgaattt
    16381 tttttacacc ttgagaactc attatgttat caatatctgt attttcatat aatgcttgta
    16441 gtactgtatt tgctactcca taactattcc ctgcataatt atatattttt tgcaatgact
    16501 cattgtataa tgtacctccc attatatcaa tttcaccatt ttctaacatc ttcattaatt
    16561 ttgtaataga ttcatttata tctccaggaa cttgaacaaa ttcataattc catccagtat
    16621 actgtgcaat ttctagtaaa tattcatatg tatatccata atagttacct tgctcatcta
    16681 tttcagttag acctttttga ataggataag caatccgaat agtacgatta tcattagcta
    16741 tagcattgat actaaaagaa aatatgtaag aagtgaatat agatgtaatt acaataatta
    16801 ataaaagtaa tttttttaaa ttcactacac aatcctccat atagttaaga tttacagtat
    16861 aaattactgc ataaacttgt agacatgaaa aaatagatta gtatataaaa ggatatataa
    16921 atttactcag aaatttaaat atccttaata tatactatat atttaaatta taactcattt
    16981 atatatctat tacaacaaat ttaccctgct aaatacctac cctatatccc ataattttac
    17041 aaattgtagt gaggctaaat tctatataac ctcaaattta gcagtgtttc tatttacagt
    17101 tcttttatat ttcacatctg gataacccag tataaatgaa gttataactg cttcattctc
    17161 gctcattcct aacaattctt ttatctttgg ctctacattt gatgccataa caaaaaatcc
    17221 attataacaa attcctaatc cttgagaata agccataagt tccatattag atgcagctaa
    17281 tcctccatct acataagcac ttccacctag agacatatca cctacaacta ccataagtag
    17341 aggagcatca taaaataaag aatcctttcc gttctcttta tatcttttgt acattttctt
    17401 aaatgtgttt ttatacatag aacgtacggg gtcattgtca tcagtattta atgctaaatt
    17461 atagagaccc tctattgcta gttcttttac ttcttttagt ttttctttta ctaatatgta
    17521 tcttataggc tgtctattcc cacctgttgg tgtgtatcta ccagcttcca ctatgttttt
    17581 tattttttct tcttctatct ctatatcttt atattgtctt atacttcttc taaatttaat
    17641 aaaattcaat aacttttcgg gctctaattc aaaactttca cgattgtatt ctactacttc
    17701 atttttatca aatccctgca tttctattgc atctgttgga caaattgcca tacagtgacc
    17761 acagttaata cagagaatat ttttcacctt tgctttatca tccttaactt ttattgcatt
    17821 tacaagacag tctgattcgc acatcccaca gcctacacat ttttctaaat ttatgtctat
    17881 cattccaaat tattcctcct tttaaaaaat ttactttagt ataaaacaaa tagttgtcta
    17941 agtcaatttt tattttttta acaatatatt agtcttaact acactttaat tgagaaatat
    18001 atattagaac ttattcaaaa tgaaagataa gaaaagtagg tgtcacaaag tgaacttttt
    18061 agttgatgag atattcttta ttgtgtgaaa tcaaggtatt tttatcattt caacaataaa
    18121 aaaggcttat ctctattttg agataaccct ttttctcaac taatgttatt tatcttaata
    18181 ttatgccaat caatgtgaac taccatccac ttaactacct acggtaatgt ttgaagtggt
    18241 ggcttcttgt tcaagtaact attgttatca gtatctccaa gctattaggc tagttcctag
    18301 ctcttatgat tgccatttat atggctaagt ttagtagatt gatacttgca ttgatatctc
    18361 tatcttaaat aatctaagaa actacacctt aattaatcta aaaactacaa tcttaattaa
    18421 tctaagaaac ttacattata atcttttagt ttcccattat tatatacaat ctttgcacta
    18481 aaaatgtttc ctgtcttagc ccaatttaag aacaacctgt cgccttccca catatttaaa
    18541 tcgaatattt tatcattatc aacccaaatt aaatttcctt ctttacagtc atttaaatct
    18601 ttttcagata attcaccttc aaattcatct gagaaataca aaaacatata ccaatcttct
    18661 tcatctttaa attttggaaa agttatcaat cctttatatg acaaagactt aagagtaaga
    18721 ccagtctctt cttttacttc cctgattaca cattcttctg gagtctctcc ttgctcaaac
    18781 tttcctccaa ctccaacata cttaccttca tgtatatcat ctttcttttt atttctgtac
    18841 aacatcaaag tcttgtcatc tttttctata taacaaatag ttgtaagttt catatatctc
    18901 gccttacttt ctctttatat tctaaactaa ttattttcag cactttcaag cacatcactt
    18961 atattcttca ttgcattaag agaaagctct gcaaattcat caaatggaat ccctatatct
    19021 tctatacttc tcattgcatc tctatttaca tttgaagcaa atctcgtttc tttcattctt
    19081 ttaatcacag acttagtttt tacactagta agttttttat ctggataaac taaagctata
    19141 gcagttataa agccagttat agggtctgct gcatatattg ctttttccat taggctagtt
    19201 cttgtaactc ccatcccatc atgatgacca agtattgctc tatacatttc ttcatcaccg
    19261 aagttttctt ttttaagcag ttctactgtt gttgtagcat gtccttcaca tatttctcct
    19321 ggctttcttc caactatatc tgaatccaaa tcatgtaaaa gacctgccac tgcccactct
    19381 tcttcttttt ctggctctaa tcttttggct aagtctctca tgacagcttc aacagcataa
    19441 caatgttttc tcattctatc agtttctaaa tacttaaata aaatttctaa agcttcttct
    19501 ctcattttca ctaatccccc tgttttttca tatatatgat atttttatat attaatacac
    19561 ataaattcaa gtttttatat atattcatgt atatttttca tgttatatct attctacacc
    19621 tttatagtag tgtcctgcaa ctgttccaat ttcatccaat tttttgtaca agtctgtagc
    19681 cttttcccca agtttataat tattactcaa gacctcaatg tgagcttcaa taaccttttt
    19741 aaccatttca ctatcttcaa ctgtaaaatc tatcctaaat acatctattc caatttcatc
    19801 taattcacat aaattgtcca acatacaagt aacctttgaa ttgtaaattg tacttctaca
    19861 gaatacatct tgtgttatcc tataacttgc atctgtgaag tctcttaaag cataattact
    19921 ttccttacat ttagcaactc tcttatcctt tttacaatct ctaacaacta ctcccatagg
    19981 acagtattct gtaaccatca taggagcata tccatatatt actgcttcag tttctacatc
    20041 tgtatatttt aaagtttgtt taatttcatt taagtttagt tcttgtgaca aacaaactgt
    20101 acttgctccc tcttctttaa agtgatttat actttcacta ttaaatgcat ttaagtagca
    20161 atctatatat aagtctttgt ccttaaaata ttccatacag ccccagttac caatttgaac
    20221 attattaatt ccttttatat tatccaatat ctcaaattgc ttatactctt tatttctgac
    20281 aattcttggt gctgaataga ttaatttctt gtcagtaact gctgtattag aacgcttatt
    20341 attcaaagta tctatgttgt ttggatttga acccattaat tctttagaaa tacttgaaga
    20401 cgaagaagta tctaaatcag tagaaatact tgaatctata gaaatacttg agaccatttg
    20461 taaagctttt gataatgtat ttatatcttc ataatagatt aagtccacat cataatttaa
    20521 tactgcttct aattgctcca aatttttgac tttaactcta atcttaggaa ctgtattaag
    20581 ttttttctgt ttatttactc tatttggctg gtattttaat atatttttct taaactttct
    20641 atcttttatc tttattcttt ctccatctaa aagctcaata caatctcttc tcatttgatt
    20701 taacacactt ataggcatac ttacaccttc gtccaagtca atttctatct cttttaactt
    20761 gtatggcgta tttccaagct tttgaatttg tgtttctatt ttttctttac ttaaagccac
    20821 ctttatagct ttttcaacta ttttgtctcc ttctactaca gatttatttc cacaaaaatc
    20881 acttataaaa agttctggtt ttttacctag tttaatactc acttttgcac ttatattagt
    20941 ttttatgaac tctttatctt gctggaatgt agcttgtact ctatccatta attcgctatc
    21001 agaagtttta tatataagct gatttcgctt tgcttctcca acaaaatcta attctatcaa
    21061 atccccttta tgggctattg tagttatctc tccatccttt attatccttc caatagtacc
    21121 tccgcctaag ttgattccat cacctttttt aagcgtattt tctagcatta ctctaagtct
    21181 tttgttcttt ctattataat ctaaaacttt tccaatatat agaccttggt tattaggtct
    21241 attagagttc attatatcct taccaacttc acctaaaata tatccttttg taaattttct
    21301 attaaatata gtatacaaat tatttattat ttcctcagaa atacttattt ttttcttatc
    21361 tacaaattca ttaatcgctt ctctatatcc tgaaaccaca gtagctacat actctggctt
    21421 tttcattctc ccttcaattt ttaaagataa tacacctgct tcaattattt tatctaaatc
    21481 ttctatagta cttaaatctc taggactcaa cagataatct ccgttacttt ttactacttc
    21541 tcctgtattt atatctatca actcatactt ttgtctgcat ggttgtgcac atctacctct
    21601 gtttccagac ctattgccta tcatactact cataagacat tgaccagagt aacatacaca
    21661 taaagctcca tgtacaaaaa cttctatatc tacagttgta ttttcacaaa tccattttat
    21721 ctcatcaaca tctaattctc ttgctaaaac aactctatca aatcctacac tctgaaggta
    21781 tttcacatct tctaaggaat gtgctaccat ttgagtgctt gcatgtagtt caaagtcagg
    21841 taatagtttt tttataagtc ttgccattcc tatgtcctgc actattaaag catcaatatc
    21901 tatatcatat aaaaatttta catactctac aaaatcttct atctcatact gttttatcaa
    21961 tgtatttaca gtaacaaaga cttgcactcc ccttatgtga gagtatttaa cagcctcaat
    22021 cagctcattt ctatcaaagt tatttgcaga tgctctggca ctaaagtctt taccacctaa
    22081 atacacagca tttgctccgt tttgaactgc tgcttttaat gaatcaaaag agcctactgg
    22141 tgcaagtaat tctatttttt tcatattttt ctcctctatt tatacttgtt aggttaatat
    22201 aattatatct ataactcaaa tatttatatt aaatttacat atttcttact atgtttaaaa
    22261 tatttgagtt aatttatata ttagttattt atacttggtt gatttgagtt aacatataca
    22321 ttatttacta tacttaggat atttctatta acttatgcac tgattaatat acttaaaata
    22381 ctttaattac tttatataat atttgctata tataaagtat ctgaacctcc catgactaaa
    22441 gtcacagggt tcctgcttca cagaattttg catactaaaa tatgtcttac taattctcca
    22501 caggccatcc cc
//
LOCUS       DAFNNB010000050        22437 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0050, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000050 DAFNNB010000000
VERSION     DAFNNB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22437
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0050"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            66..764
                     /locus_tag="L3M60_001655"
     CDS             66..764
                     /locus_tag="L3M60_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226332.1"
                     /translation="MKISSQYRSQYSFRHESNINNTRINESIVKKNETVGKDTYLSNI
                     MKQKQELNDRIRDLKDRQEVYTKKINEAIKNLYKSEIRETTNNFSNIEIGIKNSIIED
                     KNKSTMLYENSTYLNTSDEKESLITKEFNEKVEEEMLNDEKLEELEQKKDYKEDSNKK
                     EKISEDLSLAGKTREELENMLKNFTNLTQEEIMKLESRIEKLDKDAEEYKQNSKTNIF
                     DKTDEQKKHINVLL"
     gene            complement(1343..2299)
                     /locus_tag="L3M60_001656"
     CDS             complement(1343..2299)
                     /locus_tag="L3M60_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6226333.1"
                     /translation="MKNNTTQKRLRDLSKKIWNIYILNPEEEGIKFIISLLDDNLTLI
                     GTGKHEFYETLEEFIIGLEKDQVESNQIDFEIIDEWYSCTKVTDETFIVYGTLWVIEK
                     DYLEKDIYVEMDTRFTMVYRVKDDIIKLIHVHHSIPNFDQMKDEYYPKTITEKAQEAL
                     AMANEFKLKSEKDLMTGLYNRHFFEVHIKEMLKTVSHGYFYIFDLDNFKQVNDIFGHI
                     RGDEILIHFSNILTKVFISDAIIGRLGGDEFAVFEYSNQSKEDVENKAKKVLELCLEN
                     NSCSIGISRLENNSDTFQSLYKQSDYALYYSKRNNKGNFSWF"
     gene            3002..3997
                     /locus_tag="L3M60_001657"
     CDS             3002..3997
                     /locus_tag="L3M60_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6226334.1"
                     /translation="MSIIELNELTKSYRRFEKKAGFIGSVKSLFNRKYTEKTAISSFD
                     LKIEEGEFVGLIGQNGAGKTTLIKMLTGIIAPSSGEISVMGYYPNKLENNFKKQYAIV
                     MGQKSQLFFELTPADTFLLFKELYDISNEEYKKNLDYFIELFEVSEYLNVQVRTLSLG
                     ERMKMELIVALLHNPKILFLDEPTIGLDAVAQKQIRYFLKEVNETKGTTIILTSHYME
                     DIKSLCSRSIVVENSRKIYDGSTEELFNHFQKYKKITVSFSNETNIVFSKDCKVVEQT
                     NHKTSVVVPKERGNETIKWIFDRYDVKDVMIEEEDIVNIVERIYKKGSDEYYEQL"
     gene            3975..4838
                     /locus_tag="L3M60_001658"
     CDS             3975..4838
                     /locus_tag="L3M60_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBN6226335.1"
                     /translation="MSIMSNYNEASSNSSMFYKFKQSIRRYYEVAKINFKVQLIWRFD
                     VVVNMMFTIVKILFAYILWKAIFGENKIVSGFTFNSMLSYYIISSFLSGIDMSKSTSE
                     EICNRVKLGTFSKYMIIPINVQNYFIAKNFGMTCFYFVFNMISAFVWIFIFKINFAFT
                     SNPIQILISIFMIFLGLIFMMKLHFFIGILSFKFLEISFFRMIIENIVEFITGTMIPL
                     VLLPETIVKVMKVFPFYYTTYLPSMLLIGRNSNEAIQGLIILALWVLFFSVVNKVMYK
                     RMRTLFDGVGI"
     gene            4835..5629
                     /locus_tag="L3M60_001659"
     CDS             4835..5629
                     /locus_tag="L3M60_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HBN6226336.1"
                     /translation="MMKKNLKIIFMLLKMKLSKMMVFRFDFFGAFFVDSSLFILQLLM
                     FNSIYSHVDSIGGWQQGEMLIFIGTFSLINAINMTIFFFGIYDIPRKIQEGELDYYIT
                     KPIDPLFRLTFENINIGSSPLILASILIVLYGISELNSEITLVIIIIYIFMVLLMTLL
                     FYDVCIILRTVSFFVISSSAIMRLEDNLLPMNMKIPGVIYEGVFKVLFYLILPYGIMS
                     TIPTQILAGTITINGLIYSILIVFSFTLFMWRFWKLGLRHYKSASS"
     gene            5912..7291
                     /locus_tag="L3M60_001660"
     CDS             5912..7291
                     /locus_tag="L3M60_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6226337.1"
                     /translation="MSDVYYSENHEQDNLYEQDNLLAQRFNLFTLLKFAFPTIFMMIF
                     MGLYTIVDTIFVSRFINTNALSAINIVCPIINLIVGIGTMLATGGSAIVARKMGNGDI
                     EGANKAFSLIVLSGVVIGVIITGIGIVFMDYIIIWLGASEVLIKYCHSYIFIILIFAS
                     ANILQVLFQCLFVTAGKPQFGLWLVLGAGCANIILDYVFIVLLQMGISGAALATGVGY
                     LIPTISGMVFFSKNKGVLKFCIPRLDKRILFEACFNGSSEMVGQLSTAVTTFLFNAMM
                     MKLISEDGVAAITIIIYSQFLLTSLYIGFSMGVAPIISYNYGSENNTQLNHVFKICIL
                     FISVASLLVFIFSISCSPIIVEIFSQRGSRVYEIARSGFYIFSFSFIFCGYNIFTSSM
                     FTALSNGKVSAILSFLRTFGFITIGLLILPNMLNINGIWLAVPSAEILTLFLTVFCIC
                     KHKNRYKYM"
     gene            7741..8454
                     /locus_tag="L3M60_001661"
     CDS             7741..8454
                     /locus_tag="L3M60_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226338.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSSVIKIGDITIDIEGNRVYKDRKEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(8731..10269)
                     /locus_tag="L3M60_001662"
     CDS             complement(8731..10269)
                     /locus_tag="L3M60_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBN6226339.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVIPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQESAISGNEQHLIKASDYQEQLIANNFYSIDNEVNKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHEVLLLDEPTNFLDKEHVEWLSNYLNTFSGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKLHLREDYIRRYQSQQKKIEKEEAFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPGFTGKPNIKFSEIEISAQNALTVTNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPCISGDFQFSEQVKLAYYEQDL
                     KWENPTKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLFPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            11277..11563
                     /locus_tag="L3M60_001663"
                     /pseudo
     CDS             11277..11563
                     /locus_tag="L3M60_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            11551..11877
                     /locus_tag="L3M60_001664"
     CDS             11551..11877
                     /locus_tag="L3M60_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBN6226340.1"
                     /translation="MSSYLNLIREFNLKEKIIAFICVSALLIGKSCVLKNRKATTYHL
                     KNSKRQRQLSEFDVNVVNEPTVVNQNIITSYYTETAPHVAFKLLEMLISKKQLDEVKL
                     AMGFKL"
     gene            complement(11882..12205)
                     /locus_tag="L3M60_001665"
     CDS             complement(11882..12205)
                     /locus_tag="L3M60_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226341.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            12357..12875
                     /locus_tag="L3M60_001666"
     CDS             12357..12875
                     /locus_tag="L3M60_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6226342.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLDKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGLNKLSKAANIYDAPLAILVCGDKEKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKVADSPDRHEKTR
                     VPLSEIVSYEIL"
     gene            complement(13066..13734)
                     /locus_tag="L3M60_001667"
     CDS             complement(13066..13734)
                     /locus_tag="L3M60_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226343.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKNSTIKN
                     IIEKHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIDLANGSEHFYGIGAKEQILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKYYISSAFPDLKINS
                     IDIF"
     gene            13849..15264
                     /locus_tag="L3M60_001668"
     CDS             13849..15264
                     /locus_tag="L3M60_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN6226344.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILNGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNYIFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRAEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGVKVDDKGINTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKYATENGYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFILLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIEKILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHINQGT"
     gene            15487..15945
                     /locus_tag="L3M60_001669"
     CDS             15487..15945
                     /locus_tag="L3M60_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226345.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNIVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITAGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKYISYANMNCKQRIDEINSYLNFNINCEVKVA"
     gene            15942..16526
                     /gene="clpP"
                     /locus_tag="L3M60_001670"
     CDS             15942..16526
                     /gene="clpP"
                     /locus_tag="L3M60_001670"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBN6226346.1"
                     /translation="MSLVPHVIEQTSQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSVTAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRYALPNAEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDRIFSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            16829..17905
                     /locus_tag="L3M60_001671"
     CDS             16829..17905
                     /locus_tag="L3M60_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6226347.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGSPTLSYYRERLFKRSSNHKWVDPIHEVVPLSGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLEPRHQFYYSRELYYNAKYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPYIQLSVCYDRLGESNKAIYYHEK
                     TKEIKPNDSAVLHNENYFNKFKNS"
     gene            17972..20020
                     /locus_tag="L3M60_001672"
     CDS             17972..20020
                     /locus_tag="L3M60_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore surface glycoprotein BclB"
                     /protein_id="HBN6226348.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNSGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGGTGRGGPT
                     GPTGPTGPTGPTGPIGFGITGPTGATGATGATGADGVTGPTGPTGATGANGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGATGLIGPTGATGTPGVTGPTGAVGATGIGITGPT
                     GPTGATGADGATGATGPTGATGTTGADGVTGPTGATGATGFGVTGPTGPTGATGVGVT
                     GATGLIGPTGPTGATGLIGPTGVTGATGADGVTGPTGPTGATGADGATGVTGPTGATG
                     ATGADGLVGPTGATGATGADGVTGPTGATGATGVGVTGATGATGATGPTGADGLVGPT
                     GATGNTGADGVAGPTGATGATGNTGADGATGPTGATGNTGADGATGPTGPTGATGVAG
                     VTGATGPTGATGATGADGATGPTGATGATGADGLVGPTGATGATGITGATGVTGPTGP
                     TGASAIIPFASGIPLSLTTIAGGLVGTPGFVGFGSSAPGVSIVGGVIDLTNAAGTLTN
                     FAFSMPRAGTITSISAYFSTTAALSIVGSTITITATLYQSTAPNNSFTAVPGATVTLA
                     PPLTGILSVGSISSGIVTGLNIAATAETRFLLVFTATASGLSLVNTVEGYASAGISIN
                     "
     gene            20189..20950
                     /locus_tag="L3M60_001673"
     CDS             20189..20950
                     /locus_tag="L3M60_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase"
                     /protein_id="HBN6226349.1"
                     /translation="MKNKIETLMNNFVKDYHKFNSITTKWQELLFAYASAEDDMFQKL
                     KSVVGTSHAMPKDFLKNGKTIITYFIPFDESINNSNIGGFECSEIWARAYIETNMLIS
                     NLNSYLCEELEKIGFDSYAIPGSYNFDSEKLVSDWSQRHVAFISGLGTFGLNNMLITE
                     KGCCGRIGSIITSLELEPTKRPENEYCLYKHNNSCKKCVNKCVKEALKEDRFDRNKCY
                     EMCTINEKKYTYIGGCDICGKCIVGVPCSVINPSR"
     gene            21255..21533
                     /locus_tag="L3M60_001674"
     CDS             21255..21533
                     /locus_tag="L3M60_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226350.1"
                     /translation="MSYKKAIHILPEELLELIQEYVDGEYIYIPRKSNNKKEWGSNTS
                     TREELAIRNMQIYEDYQVGYDLQYLSEKYYLSLKSIQRIVLQEKRKDI"
     gene            21828..22403
                     /locus_tag="L3M60_001675"
     CDS             21828..22403
                     /locus_tag="L3M60_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1062 domain-containing protein"
                     /protein_id="HBN6226351.1"
                     /translation="MQKITWEVEYTSQLPVIRYCKKCGKKTEYVCSGLFRVNAQHKYL
                     DIWLIYKCLNCDSTWNSTIYSRVNPNNLSPEVLEQFHTNDDNLANQYAMNIELLHRNG
                     AEVGIPEYKIVGQEIDINDLTQIRIVSKYPSQLKVSTLLREKLGLSRKVFDEMLDCGT
                     IKSVSGVDLRRSKLNYEIVLEVGKEKQKSSN"
BASE COUNT         8366 a         2729 c         3701 g         7641 t
ORIGIN      
        1 taaaatataa ttaagttaag ttggcttttt ataaaacata aatttgtttt agagaggtga
       61 aataaatgaa aatatctagt caatatagaa gtcaatattc atttagacat gaaagtaata
      121 taaataatac aagaataaat gaaagtatag ttaagaaaaa tgaaactgta ggaaaagaca
      181 cttatttatc taatattatg aaacaaaagc aagaacttaa tgatagaatt agagatttaa
      241 aagatagaca agaggtttat actaaaaaaa taaacgaagc aattaagaac ttatataaat
      301 cagaaataag agaaacaact aataactttt ctaatataga aataggtatt aaaaatagca
      361 ttatagaaga taaaaataaa agtacaatgt tatatgaaaa ttcaacttat ctaaatacaa
      421 gtgatgaaaa agaatcttta atcactaaag agtttaatga aaaagttgaa gaagaaatgt
      481 taaatgatga aaaattagaa gagttagaac aaaaaaagga ttataaagag gattctaata
      541 aaaaagagaa gatatctgag gacttatcct tagcaggtaa aactcgtgaa gagcttgaaa
      601 atatgcttaa aaattttaca aatttaacac aagaagaaat aatgaaactt gagtcgagaa
      661 tagaaaagtt agataaagat gctgaagaat acaaacaaaa ttcaaagact aatatatttg
      721 ataaaacaga tgaacaaaaa aaacatataa atgtactgct ttaaacaata tgttttaata
      781 aataagttta ttatattatg aaaacataaa ggtacaattt caatacactt ctatttttgg
      841 tggaacatta gattttgatt accttaagtt aaaggatgcg acgagagaga tgactactta
      901 ctatgatgct aaggcattgt ctatattagg atttaatcaa taaaaatatg gaaggcttct
      961 tgtggaatct tttttttatt aatttgaaat ttaataagga attttatatt aaatttaatc
     1021 aataaaagta aaaaaggcta tttatagagc ctttttaagt atttttttag tagaattttt
     1081 tatatcagca tcagttttaa tcaaaaaata taatattttg gtgttatttt taaaaataaa
     1141 attattaaaa tataatatta atcatgttaa taatattttt agtttataat attctataat
     1201 agtgggtatg atgactattg tagctctatt aataatagtt ggtttatata aatatatgca
     1261 aagataggtt gttgtttacc ttacctatta gtacgtaaaa caagatactc tgagaaattt
     1321 gcctatttta atatcttttg cactaaaacc aactaaagtt tcctttatta tttcgttttg
     1381 aataatataa agcataatcc gattgtttgt ataaagattg gaaagtatca gagttatttt
     1441 ctaatctaga tatacctata gaacaagaat tattctctaa gcacaattct aaaacttttt
     1501 ttgctttatt ttcaacatcc tcttttgatt gattactata ttcaaaaaca gcaaattcat
     1561 ctccaccaag tcttccaata atagcatcac ttatgaagac tttagtcaaa atatttgaaa
     1621 agtgtattag tatttcatct cctcgaatat gaccaaagat atcatttacc tgtttaaaat
     1681 tatctaaatc aaagatataa aagtatccgt gtgaaacggt tttaagcatt tcttttatgt
     1741 gtacttcaaa aaaatgtctg ttgtatagtc cagtcataag gtctttttct gattttagtt
     1801 taaattcatt tgccattgct agtgcttctt gtgctttttc agttattgtt ttagggtaat
     1861 actcatcttt catttggtca aagtttggaa tagaatggtg aacatggata agtttaatta
     1921 tatcatcttt aactctgtat accatagtaa agcgagtatc catttctaca tagatatctt
     1981 tttctaggta atctttttct attacccaaa gagtaccata tactataaaa gtttcatctg
     2041 tgactttggt acatgaatac cactcatcta taatttcaaa gtctatttga tttgattcta
     2101 cttggtcttt ttcaagacct ataataaatt cttctaatgt ttcgtaaaat tcatgttttc
     2161 ctgttccaat taatgtaagg ttgtcatcta acaaagaaat aataaattta atgccttctt
     2221 cttctggatt taaaatataa atattccaaa ttttttttga taaatctctt aatctttttt
     2281 gtgttgtatt atttttcata gattaccccc ttaagattta cagtttatat attaatgtat
     2341 tataagttga acccttatta caatcttatt ataatataga actgaagaaa ttacatcctg
     2401 tataggtaaa aagtatatct tttatgataa aaagattaaa gaaaaataac aagttttaat
     2461 atgcatatta tattcttaat ttataaatga atattataaa tgaaatttaa attcaaccct
     2521 gaggaaataa actaaatata ttagtatatg aatctatact tgaacataaa taacctttat
     2581 gatatgatgt tgataataat attatgagag gagtgccttg atgtcggagt tgtataatgg
     2641 taaccaacaa ttaaatagtt ggagacacat atattctgta taatcagaaa ttttaagtgt
     2701 atttttaata catttaattt gtgtgtctta ttctgccttt atagataact gacatatgac
     2761 aacaaatagt atttaaaata tatattactt tgttaaatta tggtgtagta ggttttaatc
     2821 tgctacactt ttattaattt tgtatagtat tattttgata actatgttga aatagagagc
     2881 attaactttt ctagtccaca ggagttttct gtttttgtgc tgttaaatat aagcatagaa
     2941 aaaggcagat atatacctgt ggattttttt atataaatta tgaaatatgg aggcgattaa
     3001 aatgagcata attgaactta atgaactgac taaatcttat agaagatttg agaaaaaggc
     3061 aggatttata gggagtgtaa agtctttatt taataggaaa tatacagaaa aaacagctat
     3121 atcatcattt gatttaaaga tagaagaagg agaatttgtt ggattaatag gtcaaaatgg
     3181 agctggaaaa actactctta ttaagatgtt aactggtatt attgcaccat cttctggaga
     3241 aatatctgtt atgggttatt accctaataa attagaaaat aattttaaaa aacaatatgc
     3301 tattgttatg ggtcaaaaaa gtcaactatt ctttgaattg acaccagcag atacgtttct
     3361 tttatttaaa gaattatatg atatatctaa tgaagaatat aaaaaaaatt tggattactt
     3421 tatcgaatta tttgaggtat ctgaatattt aaatgttcaa gttaggacac tttccctagg
     3481 agaaagaatg aaaatggaac taatagtagc ccttctacat aatcccaaaa tattattttt
     3541 ggatgaacca actataggtt tagatgctgt agcccaaaaa caaattaggt attttctaaa
     3601 agaggtaaat gaaacaaaag gaactactat tattcttact tctcattata tggaagatat
     3661 taagagtctc tgtagtagaa gtattgttgt agaaaatagc cgtaaaatat atgatggctc
     3721 tacagaggaa ctatttaatc attttcaaaa atataaaaaa ataacagtta gtttttccaa
     3781 tgaaactaac attgtatttt caaaagattg taaagttgtt gaacaaacaa atcataagac
     3841 ctcagtagtt gtgccaaaag aaagaggaaa tgaaacgatt aaatggattt ttgatagata
     3901 tgatgttaaa gatgtaatga ttgaagaaga agatatagtt aatattgtag agagaatata
     3961 taagaaagga agtgatgagt attatgagca actataatga agcaagttca aattcttcaa
     4021 tgttttataa atttaaacag agtattagaa ggtattatga agtagccaaa attaacttta
     4081 aagtgcaact tatctggaga tttgatgtgg tagttaatat gatgtttacg attgtaaaaa
     4141 ttttatttgc ttatatttta tggaaagcaa tttttggcga aaataaaata gtctctggtt
     4201 ttacatttaa ctctatgttg tcttactata taataagctc atttttatca ggtattgata
     4261 tgtcgaagag taccagtgaa gagatatgta atcgagtaaa attaggcaca ttttcaaagt
     4321 acatgattat tccaattaat gttcaaaatt attttattgc taaaaatttt ggaatgacat
     4381 gtttttattt tgtatttaat atgatttctg catttgtatg gatttttata tttaaaataa
     4441 attttgcctt tacatcaaat ccaattcaaa tattaatttc aatttttatg atttttctag
     4501 gacttatttt tatgatgaaa ctccacttct ttattgggat tttgtcattt aaatttcttg
     4561 agattagttt ttttagaatg ataatagaaa atattgttga atttattaca ggaacaatga
     4621 taccacttgt gcttttacca gaaactatag taaaagttat gaaggtattt ccattttatt
     4681 atacaacata tttgccatct atgttactaa taggtagaaa cagcaatgaa gcaatacaag
     4741 gattaattat tttagcatta tgggttttat tcttttctgt agtaaataaa gttatgtata
     4801 aaagaatgag aacattattt gatggggtgg gtatatgatg aaaaaaaatt tgaaaataat
     4861 atttatgtta ttaaaaatga aattatcaaa gatgatggta tttagatttg atttttttgg
     4921 agcatttttt gtagatagtt cattatttat attacaactt ctaatgttta atagtatata
     4981 ttctcatgta gactcaatag ggggatggca acaaggagag atgttaatat ttataggaac
     5041 tttctcatta ataaatgcga taaatatgac gattttcttt tttggtattt atgatattcc
     5101 acgaaaaatt caggaaggag aattagacta ctatattaca aaaccaatag acccactatt
     5161 taggttaaca tttgaaaata ttaatattgg ttcatcacca ttaattttag caagtatact
     5221 tattgtttta tatggaatct ctgaattaaa ctcagagata actttagtca taataataat
     5281 atatattttc atggtgctat tgatgacatt acttttttat gatgtatgta ttatacttag
     5341 aacagtttca ttttttgtta tatcatcttc tgcgataatg agattagaag ataatttatt
     5401 gccaatgaat atgaaaattc ctggtgtaat atatgaaggt gtttttaaag tattattcta
     5461 tttaatctta ccatatggaa ttatgtccac aattccaact caaattttgg ctggaacaat
     5521 aactattaat ggattaatat attcaatttt aattgtattt tcgtttacat tgtttatgtg
     5581 gagattttgg aagttaggtc tgagacatta taaaagtgca agtagttaat aaattggcga
     5641 aaatagacaa tataattata tataaatacg tttcacatga cactagacta tgtaaacgag
     5701 tatttacact aaaggcattg aaagctataa aatgattaaa gtaaggattt aaaagttact
     5761 ttagtataaa tataattgtg taaaaaagca agattaatta aaactatatc caaataatta
     5821 gatataattt ttatgcagaa aggaggtctg taaatgtcta attgtttgga atatatttta
     5881 aaaataatta atgtacagaa aaataaacaa aatgagtgat gtatattatt cagaaaatca
     5941 tgaacaagat aatctttatg agcaagataa tttactggca caaagattta atttatttac
     6001 tctattgaag tttgcatttc ctactatttt tatgatgatt tttatgggat tgtatacaat
     6061 cgtcgatact atatttgtat caagatttat taatacaaat gctctatcag caattaatat
     6121 tgtatgtcca attattaatc ttatagttgg aattggaact atgcttgcca ctggaggaag
     6181 tgctattgta gcacgtaaga tgggaaatgg tgatattgag ggagcaaata aagctttctc
     6241 tttaatcgta ttatcaggag ttgtaattgg ggtaataatt actggtatag gtattgtatt
     6301 tatggattat atcattatat ggttaggagc aagtgaagtt ttaattaaat attgtcattc
     6361 atatattttt attatcttga tttttgcttc tgcgaatata ctacaagtat tatttcaatg
     6421 tttatttgta acagctggaa agcctcaatt tggattatgg ctagtgttag gtgcagggtg
     6481 tgctaatata attcttgact atgtgtttat tgttctgtta caaatgggta ttagtggagc
     6541 agcacttgca actggagtag gatacctaat tcctaccatt tcaggtatgg ttttcttttc
     6601 aaaaaataaa ggggtactta aattttgtat acccagacta gataaaagaa tattatttga
     6661 agcttgtttt aatggttcat ctgaaatggt gggtcaacta tcaacagcag ttactacttt
     6721 tttatttaat gctatgatga tgaaattaat tagtgaagat ggagttgcag ctattacaat
     6781 aattatttat tctcaatttc ttttaacttc gctttatata ggtttctcaa tgggtgttgc
     6841 accaataata agttataatt atggaagtga aaataataca cagttaaatc atgtttttaa
     6901 gatttgtata ttatttattt cagtggcatc tttattggta tttatctttt ctatatcatg
     6961 ttcaccaatc attgtagaga ttttttctca aagaggaagc cgagtttatg aaattgcaag
     7021 gtctggattt tatatatttt cttttagttt tattttttgt ggatataata tttttacctc
     7081 atctatgttt acagcattgt caaatggcaa ggtgtctgca atattatctt ttctacgtac
     7141 atttggattt ataactatag gtttattgat attgccaaat atgttaaata taaatgggat
     7201 atggttagca gtaccaagtg ctgaaatttt aactttattt ttaaccgttt tttgtatatg
     7261 taagcataag aatagatata aatatatgta gatattaaat aaatagttta ggtagatatt
     7321 ttatgtttta ataagtaatt caattagtgg tagatagata ctattgaaaa ttaaagttat
     7381 aattgatata aagaattaat aaaaataaca tataaaagtt taaaagggga gttgaactta
     7441 aagtttgatt agaaaaagtc gattttaaga caactccttt tattttacta ttgtattaat
     7501 ctgtatctgt aagtgtatta gtctaatatt ataaactttg gatgaaatta attttctata
     7561 ggttttaatg tatcatatac tttattttaa ggaaacgtat cttaattttc atagtgattg
     7621 aatttgtata ttagagatgt tactattagt aatgtaaaag caagagtagt gaatataaaa
     7681 aaacaagata gtgatactgc aagattagaa tataataaat ttttatggag agatttcaat
     7741 atggatacat attttaataa aaaaatatta cttgttgatg atgaaaaaga tatagtagat
     7801 ttaattgaag aagtacttat aaatgatggt tttaaaaaca ttattaaagc ttataatggt
     7861 ttagatgcta tttcgctgtg taagatagca tgtccagatg ttgtcattct tgatataatg
     7921 ttaccagata tagatggcat agaagtgtgt aaaaaaattc gagagttttc atactgttct
     7981 attctttttc tctcatcaaa aaatgatgat attgacaaaa tactaggtct tagtagtggg
     8041 ggagatgact atataacaaa gccattcagt ccaagagaaa ttgtttttcg agttaaagcg
     8101 caattacgta gacaacagta tcaaagtatt gtaccatcag atagttcagt tattaagata
     8161 ggagatatta ctattgatat agaaggaaac cgtgtatata aagatagaaa agagattgag
     8221 ttaactggaa gggagtatca tcttttaagc tacatggctc aaaatgtaaa taaaattata
     8281 ggaaaagaaa gactctatga acaagtatgg ggagtataca gtagtatttg tgacaataca
     8341 attatggtgc atatacgaca tataagagaa aaaattgaag ataacccctc aaatcctaaa
     8401 atacttataa cagttaaagg tctaggatat aaacttgtga atagaatcga ttagaataga
     8461 tcttagtatc aatgaaggtg tctcaaattc aatttgttag ttaatgagac attctttttt
     8521 gtgtgaaatc aaatgtattt ttgttatatc aataatgaag aagaggctta tctctatttt
     8581 gagatagcct cattaaattt ataccgtggt aatgcacttt atgctctaat aaatatttat
     8641 tttttagttt acttgcagtg aaacattaac taggagatta aaatttgcat tgaaatgcta
     8701 attaaacaat atcgcatatt gtatataaac ctacactaat tgtttttcta tattaaatac
     8761 tttgtcaatc cagcctttat aaaatttttc ttcatgggaa actagaatta tacttcctct
     8821 aaactgtttc aaagcatttt gtaaagcatc ctttgtttct gcatcaaagt ggttagttgg
     8881 ctcatctaaa atcaaaaaat tacaagggaa taacatcata caacataatt ttaccttaga
     8941 ttgttcacca ccacttaatg tagatacact ccttgaaaca tgttcttcct ttacgccaca
     9001 tctggctaga tgacgtctaa tctcttttgt attcaatttc ggatatttat ctgctactat
     9061 ttgaagcggt gttttagtag gattttccca ttttaaatct tgctcataat aagcaagttt
     9121 cacttgttca gaaaactgga aatctccaga aatacaagga atatctttaa ctaatgtttt
     9181 taacaaagta gatttaccaa ttccattaaa acccgttata actatttttt gacccccatc
     9241 aacagagaaa tttaattttg gaagcaaaga atagtagtag ccaacttcaa gatttgttac
     9301 tgtaagagca ttttgagcag atatttcaat ttctgaaaat tttatatttg gttttcctgt
     9361 aaaaccagga ggagctattc tttctatttt atccaattgt ttttgtcttc ctcttgcaat
     9421 ttttgaattt actccagctt tgtttttacg tataaatgcc tcttcttttt ctattttctt
     9481 ttgttgtgat tgatatcttc taatataatc ctctcttaaa tgtaattttt gtttaaggaa
     9541 ctctgaatat tttccatgat atttcttaat cataccaaat tctatatcaa gaatacctgt
     9601 agaaattttc tctaaaaagt caaaatcgtg agaaactaca ataaatgctc cactaaatgt
     9661 atttaaataa tttgagagcc attcaacgtg ttctttatct aaaaagtttg ttggttcgtc
     9721 taataataaa acctcatggt tagaaagtag caattttgct aaaattacct ttgccctttg
     9781 cccaccgcta agctccccaa caacacgatt cattcctatt gaatctaatc caagaccatt
     9841 tgctatttta tttacttcat tatctataga atagaaatta tttgctatta attgttcctg
     9901 ataatctgat gctttgatta gatgttgctc atttccagat atagcacttt cttgatagag
     9961 taaattcatc tccttttcta ttttataaag ttcttcaaat gcagtgtgta aatagagtga
    10021 tattgttgta tctctattga tttctgcata ttggtctaaa tggcctatat tgatattagg
    10081 ttgccacttg atttctcctg aatctggaat aacttctccc aataaaattt taattaatgt
    10141 acttttgcct gtaccatttt ggccaactac acctatatgt tcacctttga ataaatcaaa
    10201 gttagctttc tcatacagtg atttatccat aaaacattgt gataaattag ttacttttaa
    10261 taaactcata attcaaactc cttttctttt attaaaaaat ttatggaaat aaaaaaagca
    10321 gaggaatgac attataaaat gccgacctct gcttttggaa aaacaaaagg tcatagacaa
    10381 gcattgttgt ctatgaccag aaaatctata ttatctatcg gcaaataggt aattttaaat
    10441 aagcatagcc caaaaactac actaaaccta tatttgccga tgaagataga aagctttaag
    10501 ctttcagatt aacctctaca caacgcttca tcgtatacat ttgtgtagag ttaagttttc
    10561 taacaagttt aactaattct atataaatca taattattca catcctaatc ttagtattaa
    10621 ttctagttta tctatattta aattgtactt ttgtactttg tatattgcct tatttactta
    10681 aaatatattt tattaattat agcttaaatc atgttatcat aatacttagt tatagtcaat
    10741 aagaaagtta tatttgctac ataacctatt aatttagtaa atctatcatt acaaagttat
    10801 ttgaagactc accaccaata aaattttata atctattaaa aaattaagat tacgagattt
    10861 ataattttga tacaggtata ctatctttat taaatattgc aacatgtaga agtagatgga
    10921 taatattatt aaattttcaa tgatatgcta atgtacatat ataattatta gaaataaaaa
    10981 tataatagta tgaaagtaac tgatataata actaaatgat taaacatata gtaaaagtac
    11041 ttcataaatt atggagtatc ttttattgtc gcaaaaataa gcaatatatt atacaaaata
    11101 aaaacattaa ttcaacttaa ataattttaa gtgtaatatg tattttgatt aaataagcta
    11161 aaattctaag aaaaaataat atatttgtat taagttttat tgaaataatt aaaattattt
    11221 acttaatgta aattgttagc ttaaattata ttaaataatt aaggcggtga gtgggtctgg
    11281 atgagataga tttaaaaata attaattctc taagaaagaa tggtaggttt caacttctga
    11341 aataagcaga aaaattaatt tatcaatacc agcagtctca gagcatataa ggaaaattga
    11401 gcaattaaat ttaattcaaa agtacactat tcaaattaat caggaaaaaa taaaatataa
    11461 gcttttggct tttgtattta tcaatacgga tttatctaaa agtatggaag aatttaagaa
    11521 aactatattg gaatatgata ctgcattaga atgtcatcat atttaaatct aataagggag
    11581 tttaatttaa aagaaaaaat aatcgctttt atttgtgtgt cagcattgct gattggaaaa
    11641 agttgtgttt taaaaaatcg taaagctact acatatcatt tgaaaaatag taaaagacaa
    11701 agacagttaa gtgagtttga tgtaaatgta gttaatgaac caactgttgt aaatcaaaac
    11761 attataacct catattatac agaaacagct ccacatgtag cttttaagct tttagaaatg
    11821 ttgatatcta aaaaacaact ggatgaagtt aaattagcta tgggatttaa actgtaatag
    11881 tttagagctt ttccaagtaa tcagaacccc agtcgcacat ggaatctaaa attggaataa
    11941 tactctttcc aaaatcagat agtgagtatt ctacttttgg aggaacaaca ggataaacca
    12001 ctcttatgat taaaccatct gattctaatt cacgtaattg ttgagttaac attttaggtg
    12061 ttgccttagg gattaatttt tttaattcat tgaacctcaa tatagtgtct ttaagatgcc
    12121 aaagtataag tgtcttatat ttaccaccaa tcaatgcaag tgttgcttct attgggcaac
    12181 tgtaattatc taaacatttt ttcatttttg gactccttta tttattttaa agtatctttt
    12241 taggtactat attacaaaaa agtgcattat tgtaatatat taccatagtg ctaaaattat
    12301 agcataggtt aaataaatca tcaacttagt ctaataaaac aataggaggt aaataaatga
    12361 atttcgttga acttgcaaaa aaaagatatt cttgccgtaa ttatcaagat agaaaggttg
    12421 aaaaagaaaa acttgataag gttttagatg ttgcaagaat agctccaact ggtggtaatc
    12481 gtcaacctca aagattgatt gttattcagg aaaaagaggg tctaaataaa ctttccaagg
    12541 ctgccaatat atatgatgct ccattagcaa ttcttgtgtg tggagataaa gagaaggttt
    12601 ggacaagacc ttttgatggg aagcaactta cagatattga tacaagtata gttacagacc
    12661 atatgatgct acaagcaact gaacttggat tggcaagtgt ttgggtatgc tattttaatc
    12721 cagatataat tagagaagag tttagtttgc cagataatct agaaccaata aatattttat
    12781 tgatgggata tgaatctaag gtagcagata gcccagatag acatgaaaaa actcgtgttc
    12841 ctttaagtga aattgtttct tatgaaattc tttaattata aaattaagtt attcataggt
    12901 taaatgtaac aaagtgttta ttataaataa aactcaaaat aaataatatc cttagataaa
    12961 taatatctat ataattaatt ttagtgaagt atatctagtt taactagaat atatacttca
    13021 ctaatttatt tgttcaaaat atttgtgagg attttaatat attaactaaa atatatcaat
    13081 tgaattaatt tttaaatctg gaaaagcact tgaaatatag tattttatgt catttaaatg
    13141 gtcttgaatt tgtttttctg atattttatc aggtccatat acaaaataca taatattttt
    13201 agtatctttt gttatagctg ttcgattatc aggtccatta agaatgctag ataatatacc
    13261 aatattatcc gataaaaata aatcatcttt tgtcaaaatc tgttctttag cacctatacc
    13321 ataaaaatgt tcacttccat ttgctaagtc tattttcaaa ggtaattcta ttttatcaag
    13381 gtcatgacct gctgttagaa gaagattttt caattctgct aaaaacattg actcaattgt
    13441 tacacctaca tctgggatac cttttgattt taatagaata gattctaatt gaagcaatac
    13501 agggtaagtt ttcttgaatt ttttgtaata ctttatataa cttgaaacag gctctgtttt
    13561 aattttttct ttacgattgt attctggatg tttttctata atatttttaa ttgtagagtt
    13621 tttaagaatt aaaaaatttt ctttattagg ttttgaatgt gtaatattta taaccataac
    13681 tccaaattta atattgggat aaatttcttt cactttatgt gatatttcta acataatata
    13741 gactcctttt tattttttgt tttatgatat aatcttagca agttaaaagt agattttgta
    13801 gttccatttt tatagtaatt ttatagaacc acttttggag gtattagaat gtggattacg
    13861 attaatagaa atagtaatat atcattatca agacaaattt attgtgagat aaaagatatg
    13921 attctgaatg gaagcttaaa gtttgagcaa aaattgccat catctaggac tttatcaaaa
    13981 gaactatctg tatctcgtaa tacagtttta gatgcatata atcaattaat tgcagaagga
    14041 tatttggaaa caaggaatgg atatggaaca attgtagcta aaggaatatc agggcataaa
    14101 gttgatttta agttaaatta tatttttaga gatgaaaaag atttacatac taataaacaa
    14161 aaaaattata tagattttcg ttcaggagtt ccagaattaa aactatttcc acgcaaagaa
    14221 ctttcaaagc tatataaaca aatactgtat gatttatctg attcggattt cagatatgga
    14281 agtacagctg gtgtatgggt tttaagagcg gaaatatcta agtatttatt tcgtactaga
    14341 ggaattagat gtaatcccaa aaacattatg attgtttctg gttcaacaca aggtttatct
    14401 ttaatttcac agcttttata caaaagtaat aaaaaggttg ctgtagaaga ccctattcat
    14461 aaaggcttat taaaggttat ttcatctatt ggatattcag ttgttggagt taaagtagat
    14521 gataagggaa taaataccaa cttaataaaa tcaataaagg ggatttcttt tgtttatact
    14581 acaccttctc atcaataccc attaggaggg attttaccta ttcaaagaag actggagctt
    14641 attaaatatg caacggagaa tggttactat ataattgaag atgattatga tagtgaattt
    14701 agatatgagg gtcaaccaat tagttctctt tatgaactta acccaaataa agtaatttac
    14761 ataggttctt ttagtaaaat attatctcca gccattcgtg ttggatttat attattacca
    14821 gacaatattt tagaaagcta taaaacctta aaaatgtact ctgatgttca tacagaaaca
    14881 atattacaac atgtattagc tgaatttatt ggaaatggag gtttagagaa gtacatattt
    14941 agaatgaaga agttgtataa tagaaaacgt aagtacctca taaaagtttt aaacacggaa
    15001 tttaaaaatg aatttaaaat aaaaggtgaa gcagcagggc ttcatatagt tgctcatttc
    15061 tataatgtta aatttacgga aagtttaatt cataaaattt ttgaagcagg cataaaagtt
    15121 tatcctatac aacaatatgc aattgagaaa atattagaat atgaccatga aattatacta
    15181 ggttatgctc atcttagttt tgatgaaatt gaaaaaggtg taaaaatact cagcaaaata
    15241 atacatataa atcaaggtac atagataaaa catatagcaa tctacatata atagatgcaa
    15301 tgaagtttaa actttaaaag aaatatatat taatattttt gtataaaaat acctgatatt
    15361 gtgttatcga tactttgttg ctatacacta aattaagata gcaatttata ttaagaaaaa
    15421 actttttgca taggttataa tacttaaaag gagaggatag agttgaaaac aaatacagat
    15481 gttattatgg agataattga ttatatagaa gaaaatctta cagaaaatct aaatttagat
    15541 aaaatagctg atattgctaa ttattcaaaa taccatttac acagaatgtt tacaaacatt
    15601 gtaggatgta ctattcatca atacatccag aggaggagac tcactgaagc agctagacag
    15661 cttatattta cagacaaata tattatagac atagctatta cagcaggata ttctacacat
    15721 caatctttta cactagcatt tagaaattta tataaagaat ctccacaagt atatagaaat
    15781 aaagatgaat tttatcctat tcaacagaga tttgttttaa atctaaatca tcagattcaa
    15841 agcgaaaaat atatttctta tgcaaatatg aattgtaaac aaagaataga tgaaattaat
    15901 tcttatctta actttaatat taattgtgag gtgaaagtag catgagttta gtaccacatg
    15961 taattgaaca aacaagtcaa ggggaaaggt cttatgatat atattcaaaa ttattaaaag
    16021 atagaattat atttatagga gaagaaatta atgatgctat tgcaagttta gtagtagctc
    16081 aattactgtt tttagaatca gaagacccag attcagatat aattatatat ataaatagcc
    16141 ctggtggctc tgttacagca gggtttgcta tatatgatac tatgaattat gtcagatgtg
    16201 atgtatcgac tatttgtatt ggtatggctg caagtatgag tgctttttta cttgcaggag
    16261 gaacacatgg aaaaagatat gctttaccta atgcagagat tatgattcac cagccaatgg
    16321 gaggagctaa aggtcaagca acagatgtaa aaatagctgt agataatata ttgaaaataa
    16381 aagaacgatt agatagaatt ttttctgaaa atacaggtaa atcaatagaa gatattagaa
    16441 gagatacaga tagagataat tttatgactg cactagaagc taaagagtat ggactgattg
    16501 attatataat gaataggaat gaataaagta tcatattaaa atatctgttt tataaatata
    16561 tatagaatat taaataagag tgtctcaaag tgaacttttt agtttatggg atgctctttt
    16621 ttgtatgaaa ttaaatatag ttttattatt tcaataacta gtatcaaatt tcaagtttaa
    16681 taaattctac cataaaaata cataaaagat aaggtttttt agtaactttc tgtcgtcaaa
    16741 agtttttata aatttaataa caaaacaaga gaaaatacca tatattactt atgtctgcaa
    16801 tatatagaag acaaaggagt tgtacattat gattacaata agtttgtgca tgattgttaa
    16861 aaatgaggaa gaagttatag gcagatgctt agaatgtgta aaggatatag tagacgaaat
    16921 tataattgta gatactggtt ctacagattc cacaaaagaa atagtctcaa aatatacaga
    16981 catggtgtac gatttcgaat ggatagatga tttctctgct gcaagaaatt tttcattttc
    17041 taaagcttct aaagactata cgatgtggct tgatgcagat gacataattt tagaagctga
    17101 tagagaaaaa ttattaaaga ttaaagaaag cttggatact tccattgata ttgttatggc
    17161 aaaatacaat gtatcctttg atgaaaatgg tagcccaact ctctcgtatt atagagaacg
    17221 attgtttaaa agaagttcta atcataaatg ggttgaccct attcatgaag tagtacctct
    17281 ttctggagag gtattttatt ctgacattgc aatatctcat aaaaagcttc atagacaaga
    17341 ccctctaaga aatcttagaa tttttgaaaa aatgatttca gaaggaaaag tattggagcc
    17401 aagacaccaa ttttattact ctagagagtt gtactataat gctaagtata aagaagcaat
    17461 agaaggtttt actaagtttt tagactcatc aagaggatgg attgaggatt gtatcagtgc
    17521 ttgcagagat ttagccacat gttattactt aattaatgat gaaaagagtg ccttatattc
    17581 tttgtttaga agttttgaat ttgatgagcc aagagcagaa atttgttgtg acataggaaa
    17641 gcatatgttt gatagacaaa aatataaaga agctattttt tggtataagg tagcactaac
    17701 aagagataag aatgatacta atggaggttt taagtcaaat gattgttatg gatatatacc
    17761 atatatacaa ttaagtgtct gttatgacag gctaggagaa tcaaataaag ctatttatta
    17821 tcatgaaaaa acaaaagaaa tcaaacctaa tgatagtgct gttttgcaca atgaaaatta
    17881 ttttaataaa tttaaaaata gttgataggt cacaataata ctaaatttga catattatat
    17941 atagagctta gaagctcaaa tatatcagga ggtgctttta ataatgagta gaaataaata
    18001 ttttggacca tttgatgata atgattacaa caatggctat gataaatacg atgattgtaa
    18061 cagtggtcgt gatgattata atagctgtga ttgtcatcat tgctgtccgc catcatgtgt
    18121 aggtccaaca ggtccaatgg gtccaagagg tagaacaggc ccaacaggcc caacgggtcc
    18181 aacaggacca ggagtaggtg caacgggtcc aacaggtcca acaggcccaa caggtccaac
    18241 aggaaataca ggaggaactg gaagaggagg cccaacaggt ccaacaggtc cgacgggtcc
    18301 aacaggtcca acaggcccaa taggatttgg aataacaggt ccaacaggcg caactggagc
    18361 aacaggtgca accggagcag atggagtaac aggcccaaca ggcccaaccg gagcaaccgg
    18421 agcaaatgga ataacaggtc caacaggtgc aaccggagca acaggatttg gagtgacagg
    18481 tccaacaggc ccaaccggag caacaggagt aggagtaaca ggagcaacag gattaatagg
    18541 cccaaccgga gcaacaggaa cacctggagt aacaggtcca acaggggcag taggagcaac
    18601 aggaatagga ataacaggtc caacaggtcc aaccggagca accggagcag atggagcaac
    18661 aggggcaacg ggcccaacag gagcaacagg gacaaccgga gcagatggag taacaggtcc
    18721 aacaggtgca actggagcaa caggatttgg agtgacaggt ccaaccggcc caaccggagc
    18781 aacaggagta ggagtaacag gagcaacagg gttaataggt ccaacaggcc caaccggagc
    18841 aacagggtta ataggtccaa caggagtaac aggggcaact ggagcagatg gagtaacagg
    18901 cccaacaggt ccaaccggag caaccggagc agatggagca accggagtaa caggtccaac
    18961 aggtgcaaca ggggcaaccg gagcagatgg attagtaggt ccaacagggg caaccggagc
    19021 aactggagca gatggagtaa caggcccaac aggggcaacc ggagcaaccg gagtaggagt
    19081 aacaggagca acaggggcaa ctggagcaac tggcccaacc ggagcagatg gattagtagg
    19141 tccaaccgga gcaacaggaa ataccggagc agatggagta gcaggtccaa caggagcaac
    19201 aggagcaaca ggaaatactg gagcagatgg agcaacaggt ccaacaggag caacaggaaa
    19261 taccggagca gatggagcaa caggcccaac aggtccaaca ggggcaaccg gagtagcagg
    19321 agtaactggg gcaacaggcc caacaggagc aacaggggca acaggagcag atggggcaac
    19381 aggcccaaca ggagcaacag gggcaacagg agcagatgga ttagtaggtc caacaggggc
    19441 aacaggagca acaggaataa caggagcaac tggagtaaca ggcccaacag gtccaacagg
    19501 tgctagtgca ataatacctt ttgcatcagg tataccacta tcacttacaa ctatagctgg
    19561 aggattagta ggtacaccag gatttgttgg ttttggtagt tcagctccag gggtaagtat
    19621 agttggtgga gtaatagatc ttacaaacgc agcaggaaca ttgactaact ttgcattttc
    19681 aatgccaaga gctggaacaa taacatctat ttcagcatac ttcagcacaa cagcagcact
    19741 ttcaattgtt ggttcaacaa ttacaattac agcaacactt taccaatcta ctgcaccaaa
    19801 taactcattt acagctgtac caggagcgac agttacacta gctccaccac ttacaggaat
    19861 attatcagtt ggttcaattt ctagtggaat tgtaacagga ttaaatatag cagcaacagc
    19921 agaaactcga ttcttactag tatttactgc aacagcttca ggtctttcat tagttaatac
    19981 tgtagaagga tatgcaagtg caggaatttc aataaattag attaaataat taaaaggctt
    20041 ctagtatctt attgatatta gaggcttttt aagactgatt gataaacata gttttaatat
    20101 taattagtga gcgtaatata gagtattgtt atataatata aaataaaaac gttaaattag
    20161 aaatagagaa ttatctaggg gggatactgt gaaaaacaag attgaaacat taatgaacaa
    20221 ctttgtaaaa gattatcata aatttaatag tataactaca aagtggcaag agcttttgtt
    20281 tgcttatgca tctgccgagg atgatatgtt tcagaagtta aaaagtgttg ttggaacatc
    20341 tcatgctatg ccaaaagatt ttctgaaaaa tggaaaaact ataattacat attttatacc
    20401 ttttgatgaa agtataaaca atagtaatat tggaggattt gaatgttctg aaatatgggc
    20461 aagagcgtat atagaaacta atatgttgat ttcgaattta aatagttatt tatgtgaaga
    20521 actagagaaa ataggatttg attcatatgc tattcctggg tcatataatt ttgatagtga
    20581 aaagttggtc agtgattggt cacaaagaca cgtagcattc atatcaggtc ttggaacttt
    20641 tggtttaaac aatatgctta ttacagaaaa aggatgttgt ggaaggatag gaagtataat
    20701 cacaagttta gaattagagc caactaaaag accagaaaat gaatattgtt tatataaaca
    20761 taataatagt tgcaaaaaat gtgttaataa atgtgtaaaa gaagctttaa aagaagatag
    20821 atttgataga aataagtgtt atgaaatgtg tacgataaat gaaaagaagt acacttatat
    20881 aggtggatgt gatatttgtg gaaagtgtat agttggagtt ccttgttcag taataaaccc
    20941 atcaagataa tggatttatg tttattaata attttaaata taatttataa tgaaaaaact
    21001 ttagctatca aaataaatat aaaattaaat aaaatataat ttaaaagtta gataaccaat
    21061 ttatattgat tgacaattat ttatactgtt gatagactta tatgtatcgc tgtgatatat
    21121 aaggagggtt gagactttat gtttgattta atctttattt ttgcatagca agggctattg
    21181 caactataaa atttaatata taaatagccc tttgctattc ctaataaaaa attactattt
    21241 aataggagag caaaatgagc tataaaaagg caatacatat attaccagaa gaattacttg
    21301 aactgataca ggaatatgta gatggtgaat atatctatat tcctagaaaa tctaataata
    21361 aaaaagaatg gggaagcaat acttctactc gtgaagagtt agcaattaga aatatgcaga
    21421 tttacgaaga ctatcaagtt ggatatgatt tgcaatattt atcggaaaag tattatttat
    21481 ctttgaaaag tatccaaaga attgtattac aagaaaaaag aaaagatatc taaggatgag
    21541 ccagtatagt tttataatta tactggctca ttttcttttg ataaaagtgt ttgaggtata
    21601 gtttcctata agtagatgtt ataatttaag taatttcaat aacttataca acagataagc
    21661 aatagagttg taaagtgcat atataagaga taatagcttt ttatatgtat gaaattaaaa
    21721 tttatcaaaa actaggaggt cttgttttga aaaaaatact tgattatatt ccaccttttc
    21781 ataactttct attagtttag taactttata gaaaggaaat tatatttatg caaaaaatta
    21841 cttgggaggt ggagtacacc tctcaattac cagtaattag gtattgtaaa aaatgtggaa
    21901 aaaagactga atatgtatgt tcaggtttat ttagagttaa tgctcaacat aaatatttgg
    21961 atatatggct aatatataag tgtttaaact gtgactcaac atggaactca actatatatt
    22021 ctcgtgtcaa tccaaataat ttaagtccag aagtactaga acaattccat acaaatgatg
    22081 ataatctcgc taatcaatat gctatgaata tagaactttt acatagaaat ggagcagagg
    22141 ttggtattcc agaatataaa atagtaggac aagaaattga tattaatgac cttacacaaa
    22201 ttcgtattgt aagtaagtat ccatctcaat taaaggtatc aacattatta cgtgaaaaat
    22261 taggtttatc taggaaagta tttgatgaaa tgttagattg tgggactatt aaaagtgtat
    22321 ctggagttga tttaaggaga agtaaattaa attatgaaat tgtgttagag gtaggcaagg
    22381 agaagcaaaa gtcttcaaat tgattaaaaa atagctacgc atactcgtga ctttagt
//
LOCUS       DAFNNB010000051        22213 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0051, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000051 DAFNNB010000000
VERSION     DAFNNB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0051"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            111..1091
                     /locus_tag="L3M60_001676"
     CDS             111..1091
                     /locus_tag="L3M60_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6226352.1"
                     /translation="MSNNIKIIKKEINYFLLINFLLIAFTSIFLFLSISKPNNSELIS
                     SFSILFMYIPAFSAIVVLKKVSNYKFDSTVDKFLKFFAITTIVRIVISIAETFLIYSS
                     IISSTIDTLTSVYLLAVVLYNEFQFEVLNLSLSKNFKKVLGILLIFLILIIVRNIIDK
                     EGLSINVVNKFVTVFINIIINLCFGFNLFFGEEFGWRYFLQPRLQKIYGKKFGVLILG
                     FIWGIWHLPLCVTLYSPETPFYCVISQISYCTFIGIFLGYTYMKTKNLWAPILIHLVN
                     NTVSIILGGSIESIFTLESLLWSILVNAILFLPFLFAKEYKANNYDESSN"
     gene            complement(1307..2182)
                     /locus_tag="L3M60_001677"
     CDS             complement(1307..2182)
                     /locus_tag="L3M60_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HBN6226353.1"
                     /translation="MITFDVNGAIIRFDDTKNNYNKIRKIFKLYALEISKNFERDCLD
                     SFQNLKQISDRGLSLGEKYIEESFKKGIETIVSFGIITIDIDTFKSVYCEKYLDFKRL
                     FNNLNKTFIIPNKNKKNGHASFLEIESTVKKMSNYLYNDCFKIHYAVVDALIENRITE
                     VHSYIDEESIKKSNALFNNYKDGFIGKADECKVVKQIITLNPYREDVYEFLVKEDGDF
                     SKEIERLTEFLGYDIREYKASLMDIYINELMESDENDVENAKEKIEKYARYIGCSDEN
                     LYVARVNAIYTFANA"
     gene            complement(2364..3050)
                     /locus_tag="L3M60_001678"
     CDS             complement(2364..3050)
                     /locus_tag="L3M60_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBN6226354.1"
                     /translation="MFGVIANSLAIAGGCIVGLVIKGGLPQRVSDTIMNGIALCVLYI
                     GISGALEGKNTLVTIISVAVGALIGELIDIDKWVNKLGAFLQSKFSKGNKKDSIAEGF
                     ISSSLLFCIGAMAVVGSLESGLTGSHDTLFVKSVIDGIASIIFTASLGIGVMFSAISV
                     FLYEGIICVGASFLNGFLSDPVVTEMTAAGSLLIIGLGLNVLKLTNIKVANLLPAIFI
                     PILFGVFGII"
     gene            complement(3099..3638)
                     /locus_tag="L3M60_001679"
     CDS             complement(3099..3638)
                     /locus_tag="L3M60_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HBN6226355.1"
                     /translation="MSDLNNKIIKERDENEASMNETKIYDILNKLNVEYDVVEHEAVY
                     TAEQLVILDGITKGCQCKNLFLRNAKGNKYYLIVVKGEKQVDLNSLKDKIGSSRLSFA
                     SPERLYNVLKLLPGSVNPFSLINDTERKVELYIDKDVLKEDYLNFHPNINTKTVNISR
                     KGFEEFLEYLKYPIQSIDV"
     gene            complement(3684..4316)
                     /locus_tag="L3M60_001680"
     CDS             complement(3684..4316)
                     /locus_tag="L3M60_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HBN6226356.1"
                     /translation="MKVFDLTHVTHNDMPVYAEPNRPDIKKVAIIEENGYQETLISVF
                     SHNGTHMDSPRHMYTKGETLDKLDIENFVGKAYVLELEKGNKNIELEYLKKYEDEIKN
                     SDFIIFKSGWSKFWDKKQYYVGYPTLTKEAANYIANTNIKGIGIDMLSVDRYDTSVFE
                     VHHILFEKGKIIIENLTNLENVPEKFLFIAAPFKYNDADGAPVRAIAIVE"
     gene            complement(4403..5485)
                     /locus_tag="L3M60_001681"
     CDS             complement(4403..5485)
                     /locus_tag="L3M60_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HBN6226357.1"
                     /translation="MKIAIFLGAGASAAENLPIQNELFSEYFKKLNPTNYNTKMNREL
                     YIFFKQMFNIDIVKDNIDNANFPTFEEVLGLLDLAEQRKEAFRNFGLENLNNRSDSIR
                     FLRQYLILLMAEAIHNTPRTDNKYHKLLVENLLKNDLLLDTTFISANYDIHIDNTVTG
                     LRDKNDLPIMLDYGVEFTNFKEELNWRKPKEPLIKLYKIHGSLNWLYCPICNSVTLTP
                     HEGGVMKLIENSSETKCLECGELTEPIIVPPTYFKNMSNIFLSNVWNETEKTLRDTDL
                     LIFCGYSFPEADMHIKYMLKRVQTNRKKPPLKIMVFNNHSQKQRITLKKEEGRYKRFL
                     GEDVIFTDNSFQDFSVNPLKFIKNIY"
     gene            complement(5618..6964)
                     /locus_tag="L3M60_001682"
     CDS             complement(5618..6964)
                     /locus_tag="L3M60_001682"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBN6226358.1"
                     /translation="MKEQIKEKVNSLQDEMISSIQESVKIPSVISEATENCPFGENVD
                     KALRGILDLCKSLGFKTVYKDGYYGYAEIGNGEKMIGILGHVDVVPEGDLESWNYPPF
                     EGIVEDGKLYGRGTQDDKGPTISAIYAVKVLMDLNVDFNKRIRFIFGADEENLWRCIN
                     KYKENNEEIPNYGFTPDSRFPITNAEKGLLQVHLTCDSKSDIELSVGKALNAVPGKAI
                     YIGKYSDKLKKELDKLNFEYTVEGNKICIIGKSVHSAASDTGINAVARLCIALNNIGI
                     DSNIIKFLAEVIGEDANGNNIIPNCKDDVSGKLTVNIGRVTIDSEKEFAGIDVRIPVT
                     YKKDDFVKELKKMTDKYNLNYEEYDFLDSIYVPEDTILVKTLRKVYEEETGLDGTPLS
                     SGGATYARALDNCVAFGAIFPGKPETEHQANEYLIVEDIIKAAQIYALSIYELLKI"
     gene            complement(7033..8313)
                     /locus_tag="L3M60_001683"
     CDS             complement(7033..8313)
                     /locus_tag="L3M60_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine gamma-lyase family protein"
                     /protein_id="HBN6226359.1"
                     /translation="MLNETKELLKDYYGIDDDTFKLSQEIMEDIKGKFEEIKEIREYN
                     QYKVLKAMQESKLSDMHFNWTTGYGYNDIGREKIEEIYSKVFNTEDALVRPIIVNGTH
                     ALTLCIQGIVRPGDEILSVTGRPYDTLEGVIGIREEKGSLKEYGVTYDDVDFLEDGNL
                     DLEGIKNKINDRTKLVMIQRSKGYSWRKSLSISDIKEAIEVIKSVKPEAIVMVDNCYG
                     EFLDTKEPTDVGADVMAGSLIKNPGGGLALTGGYIAGRKDLIELISYRMTSPGIGKEC
                     GLTFGTTRNVLQGFFLAPYIVSQAVMGAIFCSRAFEKLGYDVLPKYDDLRSDIIQCIR
                     LNNADEVISFCEGIQEAAPVDSYVKPVPWDMPGYESEVIMAAGAFIQGSSIELSADAP
                     IRPPYNVYFQGGLTFDHSKMGTLKAIEFIKKLKK"
     gene            complement(8440..8697)
                     /gene="hfq"
                     /locus_tag="L3M60_001684"
     CDS             complement(8440..8697)
                     /gene="hfq"
                     /locus_tag="L3M60_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBN6226360.1"
                     /translation="MKNTVLNLQDLFLNNARKERIPVTIYLVNGVQVKGLVKGFDSYI
                     ILIEGDNRQQNMIYKHAVSTIQPGKYINLTNQNQNNNNNNR"
     gene            complement(8811..9746)
                     /gene="miaA"
                     /locus_tag="L3M60_001685"
     CDS             complement(8811..9746)
                     /gene="miaA"
                     /locus_tag="L3M60_001685"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBN6226361.1"
                     /translation="MKKIPLIILTGPTAVGKTDLSIKLAKDMNAEIISADSMQIYEYM
                     DIGSAKVTEAEMQGVKHYLIDEVKPDYPFSVSEFQQRAKKYIYEINEKGKCVLVTGGT
                     GLYLNSLIYNMDFAQSDANNELREELQNQLAEKGIDYMHNRLKELDEESANRIHKNNT
                     KRVIRALEVCLSGKKMNDFSNDLKFNEEYQPIIIVLNRDREHLYQRINMRVDIMLKNG
                     LVEEVKKLLSMGFKKDMISMQGIGYKEILKYLDGEYTYEEAIEIIKRDSRRYAKRQIT
                     WFKRYKTAKWFDLEQYENIDELKNEIILYIKDSIK"
     gene            complement(9767..11731)
                     /gene="mutL"
                     /locus_tag="L3M60_001686"
     CDS             complement(9767..11731)
                     /gene="mutL"
                     /locus_tag="L3M60_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBN6226362.1"
                     /translation="MKNIINILDDLTINKIAAGEVVERPSSVVKELIENSIDAGANKI
                     SIDIIDGGKSLIKITDNGMGIPSSEVEKSFLRHATSKIKKIDDLYDLYSLGFRGEALA
                     SISAVSKLEMTTKTKDEIIGTKIYVEGGKIISKEPIGSTNGTTIIIRDIFFNTPARQK
                     FLKSTHAETINISDLINKLAIGNPNIQFKYTNNNKQMLNTPGDGKLVNTIRSIYGKEI
                     TENIIDVEFKCNHFKMNGYIGNNNIYRSNKNLQHIYINKRFVKSKIIIDAITESYKSI
                     IPIGKHAVCFLNIEVDPSCIDVNIHPNKLEIKFEKEQEVYIELRDFLKVKLIHSNLIG
                     KYATYSDKKTQPRIAINSREKSTDYKLRNDNLLESTPKNSNTTKSKDEVIEVVTLSSE
                     KPINEFQSVSEVLNASEDEVNNINYLSEDSANDNIQEEFQVDGIRNEGNYYLGDSIKD
                     SEEEYLCSSKRKFSLYGYSVIGVVFNTYIILSKNDSMYLLDQHAAHERILYERYMEKF
                     YRQDINMQILLDPVIIEVSNVDMLQIENNLELFMKFGFELEIFGNNHIMVRCVPTIFG
                     VPETEKFILQIIDNIEEITSNYDLKGERFASMACRSAIKANDKIYDIEIKSLLEQLEK
                     CENPFTCPHGRPIMVEISKTEIEKMFKRIM"
     gene            complement(11742..14585)
                     /gene="mutS"
                     /locus_tag="L3M60_001687"
     CDS             complement(11742..14585)
                     /gene="mutS"
                     /locus_tag="L3M60_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBN6226363.1"
                     /translation="MQIDMNKLTPMMKQYLEVKNRYKDCILFFRLGDFYEMFFEDALV
                     ASKALEIALTGKACGLEERAPMCGVPFHSANSYISKLVENGYKVAIGEQMEDPSTAKG
                     IVRREVIRVITPGTVLDGNLLENKKNNYLLSLYKDGTNIGLTYVDISTGETNATCLNE
                     DKVIEEIAKIHPTEIIINDLDFIEKLRDIATVSNIYINESFSDNYLDINILKEYFPDV
                     YLQKLKFDDKGLIKSSLSILLNYIYNTQKQITSNINNINIYNSSEYMVLDMFTRTNLE
                     LTQTIRGNKKKGSLLHVLDKTSTAMGGRLLRKYVEEPLINKSKIENRLDVIEEIKDDF
                     ILREDLNDILKNIYDIERICGKIAFERVTPKELIHLKNSIEKLPNLKDTINLSNAKIL
                     KEYVSGMDKLDDIYNLIDEAILEEPTITIKDGNIIKSDFSDELKELREISKNGAFLVK
                     EIENREREKTGVKSLKIGFNKVFGYYIEITKANFKQAKLDETYIRKQTLSNAERYITP
                     ELKEIEEKILHAEEKIKSLEYEIFVEIRDTIYKNIDRIQKVAKTIANIDVFVSLATVA
                     HINNYVKPAINENNKLDIRNGRHPVVENIVGEENFVPNDTYLNRGENIINIITGPNMS
                     GKSTYMRQTAIIALMAHIGSFVPAEYADIPILDRIFTRVGASDDLSQGQSTFMVEMNE
                     VSLILKNATERSLVILDEIGRGTSTYDGISLAWSIVEYIQKNIRCKTLFATHYHELTD
                     LEEEFKEVKNYSIAVKEDGEGIIFLRKIIPQGADKSYGIYVAKLAKLPDEVIERAKYI
                     LKDLEKNHVYNSVAINGDKENNNIINSNLDEELVKVNQSNFKNKYESLKIEHELIVKD
                     YKHIKKDYDKLNSKFKVLNDEVALMKQNDDKEKTNQDDSVKEVALTQISFDSVNRDIL
                     SEEILNLDILNMTPLDAINSLYNLQRKAKEIKS"
     gene            complement(14748..15481)
                     /locus_tag="L3M60_001688"
                     /pseudo
     CDS             complement(14748..15481)
                     /locus_tag="L3M60_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428791.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(15450..16463)
                     /locus_tag="L3M60_001689"
     CDS             complement(15450..16463)
                     /locus_tag="L3M60_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226364.1"
                     /translation="MNFKKLGILGLVSVFTLATITGCSSNNNKDKNNDKNASSKKVTM
                     VLDWTPNTNHTGLFVALDKGYYKEEGLDVEIVQPPESGAETLVATGKADFGISYQEQV
                     TYAKTSEDPLPIKAVAAVIQHNTSGFASPKEKNITTAKDFEGKTYGGWGSPSEEAVFK
                     AVMKKNGADFNKLKIVNTGQDDFFAAMKTVDFAWIFEGWDAVKADLIGYDLNFIPVKD
                     LDERLDYYTPLIISNETVLKDNPELAKKFLKATTKGYEYAIKNPEEASKILVKHAPEV
                     DEKLALKSQEYLASKYKDDAPRWGEMKDSVWNNYTSFLKEYKLIDKDMKASDAYTNEF
                     LPK"
     gene            complement(16485..17255)
                     /locus_tag="L3M60_001690"
     CDS             complement(16485..17255)
                     /locus_tag="L3M60_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226365.1"
                     /translation="MKNLEIIDKVKDSIYPIATFLFILILWQSVVGVLEVPQYILPTP
                     VDIINVFFKDYQNLSMHSVVTIGEAVLGFIVAIIISLVIGILMDFVSIIKKCLYPIML
                     VTQMIPTIIIAPLFMIWFGFGTMPKVLMVMLTCFFPILISFVDGIENIDKDYLNLFKT
                     MNSNKVNTFIHLKFPMAMDKFFSGLKISATYAVMAATVAEWLGGTKGLGVYMLRSKSA
                     YALDKVFASTILVVIFSLMFVGIVQFVKKIVIRHRLTD"
     gene            complement(17230..17520)
                     /locus_tag="L3M60_001691"
     CDS             complement(17230..17520)
                     /locus_tag="L3M60_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HBN6226366.1"
                     /translation="MVIADIAVMPLRPYKGEEEMYKVVDACIAEIEKSGLKCEIGAMS
                     TTVEGDFDEVFELLKKVHKIPFNLGCERVITVARVDEKAGGLTIDEKLRNHR"
     regulatory      complement(17652..17751)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(17876..18010)
                     /locus_tag="L3M60_001692"
     CDS             complement(17876..18010)
                     /locus_tag="L3M60_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBN6226367.1"
                     /translation="MNKNKNLAQCLDGIDIYPNMLEIVKQKLENYVNLVLGDTEKLSF
                     "
     gene            complement(18025..18198)
                     /locus_tag="L3M60_001693"
     CDS             complement(18025..18198)
                     /locus_tag="L3M60_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226368.1"
                     /translation="MIFLYYSGCEPQEEPCVVEINQESDYEITYLEYNFKEFIEGIFV
                     YKDECSIKTMLNQ"
     regulatory      18720..18809
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18862..19038
                     /locus_tag="L3M60_001694"
     CDS             18862..19038
                     /locus_tag="L3M60_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226369.1"
                     /translation="MFMDKMDRCTHILTAYICSSRDYCNFIDTQLNDFILEYGENVVE
                     SCLHQVMVLVSRYN"
     gene            complement(19153..19509)
                     /locus_tag="L3M60_001695"
     CDS             complement(19153..19509)
                     /locus_tag="L3M60_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226370.1"
                     /translation="MDIDNNFEEFPNIEERQKDFNCSIGFTMTVIGSKWRAIILWHIL
                     KQEPIRYSQLKKEVVHISHKILSQELKYLESDGLIKRISYPTIPPKVEYLSTKRGKSL
                     ESILLELCNWGKQYMR"
     gene            19765..20685
                     /locus_tag="L3M60_001696"
     CDS             19765..20685
                     /locus_tag="L3M60_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBN6226371.1"
                     /translation="MNKKFRLYQVDSFTKEKFTGNPAGVITNADGLSESQMQKIAREL
                     NNSETAFIFNKLAKNHQENYDVHIRFFTPTNEVPICGHATIAAHYVRAVENQLNNKTV
                     FQKTGAGILPVDIIKKDNDYEIIMTQGKIEVSPPLQQTYQERLLNAFGILKEDIRTDC
                     PIAISSTGHSKVMIGIKELSTLNSLSPNLYELSKISQEIKCNGYYIFTLHDKETLQVH
                     GRMFAPAIGISEDPVTGNANGPLGAYLVHYKVIPIPNTEFIFEAVQGEAIKRKGKMLV
                     KVIISDNKPVEVKIIGKAVIAFSTNIEIEL"
     gene            20910..21521
                     /locus_tag="L3M60_001697"
     CDS             20910..21521
                     /locus_tag="L3M60_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBN6226372.1"
                     /translation="MINDIKNKFKDYKPYINGYKNMKRASVLIPIVEINNTHYILFEV
                     RSKNLKHQPSEISFPGGKIESGETPYEAVIRETCEELGTFSDNIEVISSLDLLITPVK
                     FIIHPYLGYIKNINNLNINKDEVDHTFLVPIKYLLENPPNTYSNTVKVFPDENLPYGS
                     IPNQKDYKFESGSYPILFYKYKDYVIWGMTAKILENFLNFLSE"
     gene            21826..22164
                     /locus_tag="L3M60_001698"
     CDS             21826..22164
                     /locus_tag="L3M60_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBN6226373.1"
                     /translation="MFAVGFVVIIALAFFILSVLLFNGKASMLLAGYNSMSKKEKARY
                     DKKKLCRSSSIVTFIVSIMLFIMAFLGYRIESGQMDEKKMVTFSIIFIIVILSSVFFN
                     ILYSNKKCKK"
BASE COUNT         7277 a         3610 c         2412 g         8914 t
ORIGIN      
        1 atatataaaa atatttttgt tagttttcaa tatttgatat atatttattt ctatataaaa
       61 tctgttatta tatacatata atttaaaaat ttaaaataag gggaattaat atgtcaaata
      121 atattaaaat aataaaaaaa gaaataaact atttcttatt aataaatttt ttactgattg
      181 cttttacatc aatattttta tttttgtcaa tatcaaaacc aaataatagt gaacttatat
      241 caagcttctc tatattattt atgtatattc ctgcattttc agcaattgtt gttctaaaaa
      301 aagtttctaa ttacaagttt gattcaacag tggataaatt tttaaagttt tttgctataa
      361 ctacaattgt gagaatagta atatctatag ctgaaacatt tttaatctat agtagtatta
      421 tttcttcaac aatagataca ttaacatctg tttatctttt agcagttgtt ctttacaatg
      481 aatttcaatt tgaagtttta aatttatctt taagtaaaaa tttcaaaaaa gttttaggca
      541 ttctattaat attcttgata ttaattatag ttagaaatat tattgataaa gaaggtttat
      601 caattaatgt tgtaaataaa tttgtcacag tatttataaa tataattata aatttatgtt
      661 ttggttttaa tttatttttt ggagaagaat ttggatggag atactttctt caacctagac
      721 tacaaaagat ttatggcaaa aaatttggtg tattaatttt aggttttata tggggaattt
      781 ggcatttgcc tctttgtgtc actttgtaca gtcccgaaac cccattttat tgtgttattt
      841 ctcagatatc atattgtacc tttataggaa tatttttagg ctatacctat atgaagacca
      901 aaaacctttg ggcgccaata ttaatccact tagtaaataa cactgtttct attatacttg
      961 gaggaagtat tgagtctata tttacattag aatcactatt atggagtatt ctagttaatg
     1021 ctatattatt tttaccattc ttatttgcta aagagtacaa agctaataat tatgatgaaa
     1081 gttctaacta gtctacaaat acatgattct ttagacatag aagcttttta tatttcataa
     1141 caaaaccctc ctagttttag acagttttac acattttaac tgtctcctaa gaggggtcta
     1201 gttcattttg agaatggttt ttaatataaa cgatttatta ttaataagga tattttttta
     1261 tttaaattta tataatcgaa catctaatat ttaatgttta ataaaattat gcatttgcaa
     1321 aagtatatat ggcattaacc cttgctacat ataaattctc atcactacat ccaatatatc
     1381 tagcatattt ttcaatcttt tcctttgcat tttcaacatc attttcgtcg ctctccatta
     1441 actcatttat ataaatatcc attaatgatg ctttatattc tcttatatca taacctaaaa
     1501 attcagtcaa tctctcaatt tccttactaa aatctccatc ttcttttacc aaaaattcgt
     1561 atacatcttc cctatatgga tttaaagtta ttatttgttt tactacttta cattcgtcag
     1621 ccttcccaat aaaaccatct ttataattat taaataaggc attagatttt tttatagatt
     1681 cttcatcaat ataagaatgt acctcagtta tacgattttc tataagagca tctacaacag
     1741 cataatgaat tttaaaacaa tcattgtata aataattact cattttcttt actgtcgatt
     1801 ctatttctaa aaaactagca tgaccgtttt ttttattttt atttggtata ataaaggttt
     1861 tatttaaatt attaaaaagt ctcttaaaat ctaaatattt ttcacaataa acacttttaa
     1921 aggtatcgat atctatagtt ataattccaa aactcactat agtctcaatg cctttcttaa
     1981 aagactcttc tatatatttt tctcctagac ttaaacctct atcagatatt tgctttagat
     2041 tttggaaact atctaagcaa tctctctcaa agtttttaga tatttctaaa gcatataatt
     2101 taaatatttt ccttatctta ttataattat tttttgtatc atcaaacctg ataatagctc
     2161 catttacatc aaaagtaatc attgagtttt aacctcccca ttagtttcat caataaaata
     2221 aatttaatat aaactttata atcataatat taaacttatt tatactataa ttattaattt
     2281 gatgataaaa gctattttcc ttctaaataa aaaatttgtc taaagtttct aaaaaattta
     2341 gacaaatctt aggttattac tttttaaatt attccaaata ctccaaataa tattggtata
     2401 aatatagctg gtaacaaatt ggctactttt atattcgtca atttaagtac atttagacca
     2461 agacctataa ttaataaact tccagctgct gtcatttctg taactacagg atcacttaaa
     2521 aatccattta aaaatgatgc tcccacacat ataatacctt cgtacaaaaa tacagatatt
     2581 gcagaaaaca ttacacctat acctaaagaa gcagtaaata taattgaagc tattccatct
     2641 ataacagatt ttacaaataa agtatcatga cttcctgtaa gaccactttc taatgagcca
     2701 actactgcca tagctcctat acagaataat aaactagatg atataaaacc ctctgctata
     2761 gaatcttttt tatttccttt agaaaattta ctttgtaaaa aagcacccaa cttatttacc
     2821 catttatcaa tatctattaa ttctcctatc aaagcaccta cagctacgga tattattgtc
     2881 actaatgtgt tttttccttc tagtgctcct gatataccaa tgtacaatac acatagggca
     2941 attccattca ttatagtatc gctcactctt tgaggtaatc ctccttttat tacaagacct
     3001 actatacatc cacctgcaat tgctagacta tttgctatta ctccaaacat tttttctccc
     3061 ctcgattagc taattgaaat ttatcctact attaaaactt aaacatcaat actttgtatt
     3121 ggatatttta aatattccaa aaattcttca aagccttttc tagatatatt cacagtttta
     3181 gtatttatat taggatgaaa atttaagtaa tcttctttaa gtacatcttt atctatgtaa
     3241 agctcaactt ttctttctgt atcatttatt aatgaaaaag gatttacact tccaggaaga
     3301 agtttaagta cattgtatag tctttcaggt gaagcaaatg ataatctaga gctccctatc
     3361 ttatctttaa gtgaatttaa atcgacttgt ttttctcctt taactacaat aagataatac
     3421 ttatttccct tagcatttct taaaaataaa tttttacatt ggcatccctt tgttattcca
     3481 tctaaaatta ctaattgctc tgctgtatat acagcttcat gttctactac atcatattca
     3541 acatttaatt tattcaaaat atcatatatt ttagtttcat tcatgctcgc ttcgttttca
     3601 tctcgttctt taataatctt gttatttaaa tcactcatac tttcttctcc ttaaaccctc
     3661 catcatcata tcattgtgag atattattct actatagcta ttgctcttac tggagctccg
     3721 tcagcatcat tatacttaaa tggcgctgct ataaatagaa atttttctgg aacattttct
     3781 aaatttgtaa gattttctat gattattttt cctttctcaa ataaaatatg atgcacttca
     3841 aatactgatg tgtcatatct atccactgat aacatgtcta ttcctatccc ttttatatta
     3901 gtattagcta tataatttgc agcttctttt gttaatgtag gatagcctac atagtactgc
     3961 tttttatccc aaaactttga ccaacctgat ttaaatatta taaaatcact attttttatt
     4021 tcatcttcat atttttttag atattctaac tctatattct tatttccctt ttcaagctcc
     4081 aatacataag ctttcccaac aaaattctct atatccaact tatctagggt ctcaccttta
     4141 gtgtacatat gtcttggtga atccatatga gttccgttgt gagaaaatac actaattaat
     4201 gtttcttgat aaccattttc ttcaataata gctactttct tgatatctgg tctattaggc
     4261 tctgcatata caggcatgtc attatgtgtc acgtgagtta aatcaaaaac cttcatcttt
     4321 atataccccc tatgttttta ttttctatta tatacctaaa ttcattttat ttctatcaga
     4381 ttttattcca ttatatacca tactagtata tattttttat aaacttcaaa ggatttaccg
     4441 aaaaatcttg gaaagaatta tcagtaaata taacatcttc acccaaaaaa cgtttatacc
     4501 ttccctcttc tttctttaaa gtaattctct gtttttgtga atggttatta aaaaccatta
     4561 tcttaagtgg aggttttttt ctattagtct gaactctttt taacatgtat tttatgtgca
     4621 tgtcagcttc gggaaatgaa tatccacaaa atattaaaag gtcagtatct cttaaagttt
     4681 tttctgtctc attccaaaca ttacttaaga atatattgga catattctta aaatacgttg
     4741 gtggcactat aataggttct gtcaattcac cacactctaa acattttgtt tcagaactat
     4801 tttcaataag tttcataaca ccaccttcat gtggtgtaag agttacacta ttacaaatag
     4861 gacaataaag ccaattcaat gacccatgta tcttatataa tttgattaat ggctctttcg
     4921 gttttctcca atttaattct tctttaaaat ttgtaaactc cacaccataa tcaagcataa
     4981 ttggcaaatc attcttatct cttaagccag taactgtatt atctatatgt atatcataat
     5041 ttgcactgat aaaagtagta tctaaaagca aatcattttt caaaagattt tcaactaaaa
     5101 gtttatgata tttattatct gttctaggtg tattatgaat tgcctcagcc attagcagta
     5161 ttaaatattg tcttaaaaaa cgtatactat cacttctgtt attaagattt tctagtccaa
     5221 aatttctaaa tgcttctttt ctttgctctg ctaagtctaa aagccctaaa acttcttcaa
     5281 aggtaggaaa atttgcatta tctatattat cttttactat atcaatgtta aacatctgtt
     5341 taaagaaaat atacaattct ctattcattt tggtattata attggtagga tttagttttt
     5401 taaaatattc actaaacagt tcattttgaa taggtagatt ctctgctgct gaagctcctg
     5461 ctcctaaaaa tattgctatt ttcatatagt tcctcctaat ataaatatta ctcctatttt
     5521 tatatccgtt aaatatccat atcatttgtt ttatgataca ataataaagc tgtcccttac
     5581 tagaaaaaat cctattacaa gacagcctta tattatatta aatctttaat aattcataaa
     5641 tagaaagagc atatatctga gcagccttta taatatcctc tactattaag tattcattag
     5701 cttggtgttc tgtttctggt tttcctggaa aaatagctcc aaatgcaaca caattgtcta
     5761 aagctcttgc ataagttgct cctccagatg aaagtggtgt tccatcaaga ccagtttctt
     5821 cttcatatac ttttctaagt gtcttaacaa gaattgtatc ttctggaaca tagattgaat
     5881 ccaagaaatc atattcttca taatttaaat tgtatttatc cgtcattttc tttaattctt
     5941 ttacaaaatc atctttcttg tatgttactg gaatccttac atctattcct gcaaattctt
     6001 tttcgctatc aatagttact ctacctatat ttactgttag tttacctgac acatcatcct
     6061 tgcaattagg aattatattg tttccattag catcttcacc aataacttct gctaaaaatt
     6121 taattatatt agaatctata cctatattat taagagctat acaaagtctg gccactgcat
     6181 ttataccagt atcactcgct gctgaatgga cacttttccc tattatacag attttattac
     6241 cttcaacagt atactcaaaa tttaatttat caagttcttt ttttaattta tcagagtatt
     6301 ttccaatata gattgcttta cctggaactg catttagagc ctttccaact gataattcta
     6361 tatcgctttt gctatcacaa gtcaaatgaa cttgtaaaag acctttttct gcatttgtta
     6421 ttgggaatct agaatctgga gtaaatccat agtttggtat ttcttcatta ttttctttat
     6481 atttatttat acatctccaa agattttctt cgtcagcccc aaaaatgaat cttattctct
     6541 tattaaaatc tacatttaaa tccataagaa ctttaactgc ataaattgct gatattgtag
     6601 gtcctttatc atcttgtgtt cctcttccat acaatttacc atcttctaca ataccttcaa
     6661 atggaggata attccaactc tctaaatcac cttctggaac cacatcaaca tgaccaagta
     6721 tacctatcat tttttcaccg tttcctattt ctgcatatcc ataatatcca tctttgtata
     6781 ctgtcttaaa acctagagat ttacataagt ctaatatacc tcttaaagct ttatctacat
     6841 tttcaccaaa tggacaattt tcagttgctt cagatataac actaggtatt tttacgcttt
     6901 cttgtattga agaaatcatt tcatcttgta gagaatttac tttttcttta atttgctctt
     6961 tcatattatc acccttcaat tttcatcata atatactaat aaatattttg acctttttaa
     7021 aaaaaaatat gcttattttt tcagtttttt tataaattca attgctttta aagtccccat
     7081 ttttgaatgg tcaaatgtaa gtcctccttg gaaataaaca ttataaggag gtcttattgg
     7141 agcatcggca cttaattcta tagaagaacc ttgtataaat gctcctgctg ccattattac
     7201 ttcactttcg tatccaggca tgtcccaagg aacaggtttc acataagaat caactggtgc
     7261 agcttcttgt ataccttcac aaaaacttat tacttcatca gcattattaa gtctaataca
     7321 ttgaataata tcacttctta agtcatcgta ttttggtaat acatcataac ctaatttttc
     7381 aaaagctctt gaacaaaaga tagctcccat tacagcctga gatactatgt aaggtgctaa
     7441 gaagaaacct tgaagtacat ttctagtagt tccaaaagta aggccacact cttttcctat
     7501 tccaggagaa gtcattctat aagatataag ctcaattaaa tcttttctac cagctatata
     7561 tccacctgtc aatgcaagac caccaccagg attttttatt aaagaaccag ccataacatc
     7621 tgctccaaca tcagtaggct ctttggtatc taaaaactca ccataacaat tatcaaccat
     7681 tactatagct tctggtttaa cggactttat tacctctata gcttctttta tatcactaat
     7741 tgaaagcgat tttctccaag aatacccttt tgacctttgt atcataacaa gtttagttct
     7801 atcatttatt ttgtttttaa ttccttctaa atctaagttt ccatcttcta aaaaatctac
     7861 atcatcatat gtaacaccat actctttcaa tgaacctttt tcttctctta tacctataac
     7921 tccctctaaa gtatcatatg gtctaccagt aacagataaa atttcatctc ctggtctaac
     7981 tataccttgt atacataaag taagtgcatg tgtaccattt acaattattg gtcttacaag
     8041 agcatcttct gtattaaata ctttagaata aatttcctct attttttcac gaccaatatc
     8101 attatatcca tatcctgtag tccagttaaa gtgcatatca cttagtttag attcttgcat
     8161 agcttttaaa actttatatt gattgtattc tcttatttct tttatttctt caaactttcc
     8221 ttttatatct tccattattt cttgagatag tttaaaagta tcatcatcta tcccatagta
     8281 gtctttcaat aattcttttg tctcattaag cattttatca cctgtctctt tattgtttta
     8341 tttaaatatg taaaggagac tattttacta aaacaaataa attatagttt atcagttagt
     8401 ataagtctcc tctcaattat cttaaattaa attagttatc tatctgttgt tattattatt
     8461 attttgattt tgatttgtta gattaatata ctttccaggt tgtatagttg atacagcatg
     8521 tttataaatc atgttttgtt gtctattatc cccttctatt aatattatat aactatcaaa
     8581 cccctttaca agccctttaa cttgcactcc attgactaag tatatagtaa cgggtatcct
     8641 ttctttcctt gcattattta aaaacaaatc ttgtaaattt aaaactgtat ttttcattag
     8701 atttgtaccc tcccaagatt aaattgaact tctaaaatat tcctacctta tattctatat
     8761 aagttatgac aaattttcaa tattaagttg tatacttaaa aatttatata ttattttata
     8821 gaatctttaa tatataagat tatttcattt tttaattcgt ctatattttc atattgctct
     8881 aaatcaaacc actttgctgt tttatatctt ttaaaccatg ttatctgacg ttttgcatac
     8941 cttctagaat ctcttttaat tatttcaata gcttcttcat atgtatattc tccatccaga
     9001 tactttaata tctccttata gcctatacct tgcattgata tcatatcttt tttaaatccc
     9061 atacttaaaa gtttttttac ctcttcaacc aatccatttt taagcataat atcaactctc
     9121 atatttattc tttgatataa atgttctcta tctctattta gaactattat tatcggttga
     9181 tattcttcat taaattttaa atcattggaa aaatcattca ttttcttgcc acttaggcaa
     9241 acttcaagag ctcttatgac ccttttagta ttgtttttat ggattctatt agcagattct
     9301 tcatcaagtt cttttagcct attatgcatg taatctattc ccttttcagc aagttgattt
     9361 tgaagttctt ctcttaattc attattagca tcagactgtg caaaatccat attatatatt
     9421 aaagagttta aataaagtcc tgtgcctcca gttactaaaa cacattttcc cttttcattt
     9481 atttcatata tatatttttt tgctctttgt tgaaactctg aaactgaaaa tggatagtcc
     9541 ggtttaactt catctataag gtaatgcttt actccttgca tctctgcctc tgtaactttt
     9601 gcacttccaa tatccatata ttcatatatt tgcatagaat cagctgaaat tatctctgca
     9661 ttcatatctt ttgctaattt tattgacaaa tctgtttttc caacagcagt tggacctgta
     9721 agtattataa gtggtatttt tttcatagca ttcctttcca tatattttac ataattcttt
     9781 taaacatttt ctctatttct gtcttagata tttcaaccat tataggtctt ccatgaggac
     9841 atgtaaatgg attctcacac ttttctagct gttccaataa actttttatt tcaatatcat
     9901 atattttgtc atttgctttt atagcagacc tacatgccat agatgcaaac ctctctcctt
     9961 ttaaatcata attacttgta atttcttcta tattatcaat tatttgaagt ataaatttct
    10021 cagtttcagg cactccaaat atagtaggaa cacatcttac cattatatga ttatttccaa
    10081 atatctctaa ttcaaaacca aatttcataa ataactccaa attattttca atttgaagca
    10141 tatctacatt tgatacttca ataatcactg ggtctaaaag tatttgcata ttaatatctt
    10201 gtctatagaa cttctccata tacctttcat acagtattct ttcatgtgca gcatgttggt
    10261 ccaacaaata catagaatcg tttttgctca aaattatata ggtattaaat acaactccaa
    10321 ttacagaata tccatataag gaaaacttgc gttttgagct acacaagtac tcttcttcac
    10381 tatccttaat actatctcct aagtaataat ttccttcatt tctaattcca tcaacttgaa
    10441 attcttcttg tatattgtca tttgcactgt cttcacttag gtaattaatg ttatttactt
    10501 catcttcact tgcatttaag acttcactta ctgattgaaa ttcatttatt ggtttttcac
    10561 tcgataaagt tacaacttca ataacttcat ccttactttt agtggtatta ctatttttag
    10621 gagtagattc taataaatta tcatttctta atttataatc tgtacttttt tctctactgt
    10681 taatagcgat tctaggctgt gtttttttat cactataagt agcatatttt ccaataagat
    10741 ttgagtgtat taacttaacc tttaaaaaat ctctaagttc tatatacact tcttgttctt
    10801 tttcaaattt aatttcaagt ttattgggat gtatatttac atctatacaa gatggatcta
    10861 cttctatatt taagaaacaa actgcatgtt ttcctattgg aataatagat ttataacttt
    10921 ctgttattgc atctattatt attttacttt ttacaaacct tttatttata tagatatgtt
    10981 gtaggttttt attagaacgg tatatattat tattacctat gtatccattc attttaaagt
    11041 gattgcactt aaattctaca tctattatat tctctgttat ttcttttcca tatatacttc
    11101 taatcgtatt tacaagcttc ccatctcctg gagtatttag catctgctta ttgttatttg
    11161 tatatttaaa ttgaatgttt ggattaccta ttgcaagttt atttatcaaa tcacttatgt
    11221 ttatagtttc tgcatgagta gattttaaaa atttttgtct tgctggtgta ttaaagaaaa
    11281 tatctcttat aatgattgta gtcccattag tagagcctat gggttctttt gatattattt
    11341 ttccaccttc aacatatatt ttagttccta ttatttcatc tttagttttg gttgtcattt
    11401 ctaattttga tacagctgaa atagatgcca atgcttcccc tctaaaacca agagagtata
    11461 aatcatacaa atcatcaatt tttttaattt tactagtagc atgtcttaaa aacgattttt
    11521 caacttcact tgaaggtatt cccattccat tatctgtaat cttaattaga ctttttcctc
    11581 catcaattat atctatagaa attttatttg cacctgcatc aatggagttt tcaataagtt
    11641 cttttactac agaagaaggt ctttctacaa cttcacctgc tgctatctta tttatagtca
    11701 aatcatctag gatgttaatt atatttttca tttttacacc tctaagattt tatttctttt
    11761 gcttttcttt gaagattata aagagagttt atagcatcta atggagtcat atttaaaata
    11821 tccagattta aaatttcttc tgatagtatg tctctattta cactatcaaa agatatttga
    11881 gttaaggcta cttcctttac agaatcatct tggttagttt tctctttatc atcattttgc
    11941 ttcattaaag caacttcatc atttaatact ttaaatttag aatttaattt atcataatcc
    12001 ttttttatat gcttgtagtc ttttacgata agttcatgtt ctatttttag agattcatat
    12061 ttattcttaa aattactttg atttacctta actaattctt cgtctaaatt actattaatt
    12121 atattattat tttctttatc tccatttatt gcaacactgt tgtaaacatg atttttttcc
    12181 aagtctttaa gtatatattt tgctctttct ataacttcgt ctggtaattt agccaattta
    12241 gcaacatata ttccataact cttatctgct ccctgaggaa ttatttttct taaaaatatt
    12301 attccttccc catcttcttt gactgctatt gaataatttt tgacctcttt aaactcttct
    12361 tctaaatcag taagttcatg atagtgtgtt gcaaataatg tcttgcatct aatgtttttt
    12421 tgaatatatt ctactattga ccatgctaaa cttatcccat cataggtact agtcccacga
    12481 cctatttcat ctaatataac taaacttctc tctgttgcat tttttaatat aagggatact
    12541 tcgttcatct ctaccataaa tgtagattga ccttgagata aatcgtcact agctccaact
    12601 cttgtaaata ttctatctag tattggaata tcagcatact ctgctggtac aaatgagcct
    12661 atatgtgcca taagagctat tatagcagtt tgtctcatat atgttgattt acccgacata
    12721 ttaggaccag ttattatgtt aataatgttt tctccacgat ttaaataagt atcatttgga
    12781 acgaaatttt cctcacctac tatattttcg acaacaggat gtctaccatt tcttatatca
    12841 agtttattat tttcatttat cgctggttta acatagttat ttatatgtgc aaccgttgca
    12901 agagagacaa agacatctat attagctata gtcttagcta ctttttgtat tctatctatg
    12961 tttttatata ttgtatctct tatctctaca aatatctcat attctaaaga tttaatcttt
    13021 tcctctgcat gtagtatctt ttcttctatt tcctttaatt ctggagttat atatctttct
    13081 gcattactaa gtgtttgttt tcttatatat gtctcatcta gctttgcctg tttaaagtta
    13141 gcttttgtta tttctatata atacccaaaa actttattaa accctatttt taatgattta
    13201 actcctgttt tttctctttc tctgttttct atctccttta caagaaaggc tccattctta
    13261 gaaatctctc ttaattcctt aagttcatca ctaaaatctg atttaattat gtttccatct
    13321 tttatggtta ttgtaggttc ttctaaaata gcttcatcta ttaaattgta tatatcatct
    13381 aatttatcca ttcctgaaac atactctttt aggatttttg catttgatag atttatagta
    13441 tcttttaaat taggtaactt ttctatcgaa ttttttaagt gaattaactc ttttggtgtt
    13501 actctctcaa atgctatctt tccacatatt ctttctatat cgtatatatt tttaagtata
    13561 tcatttaggt cttctcttaa tataaagtca tcttttattt cttctattac atccaatcta
    13621 ttttcaattt tagatttgtt tatcaaaggt tcttctacat attttcttaa aagtctacct
    13681 cccattgctg tagaagtttt atcaagtaca tgtaataatg aacctttttt cttattccct
    13741 cttatagttt gggtaagttc taaattagtc ctagtaaaca tatctaaaac catatattct
    13801 gatgaattgt atatatttat attattaatg ttagatgtta tttgtttttg agtattgtat
    13861 atataattca gaagaattga taagcttgac tttattagac ctttgtcatc aaactttagt
    13921 ttttgtagat aaacatctgg aaaatattct tttaatatat ttatgtccaa atagttatcg
    13981 ctaaaacttt catttatgta tatattactt acagtagcta tatctctaag cttttctata
    14041 aagtctaaat catttataat tatttcagtt ggatggattt ttgcaatttc ctcaattacc
    14101 ttatcttcat ttaaacatgt tgcattagtt tctcctgtac ttatatcaac ataagttaga
    14161 cctatattag taccatcctt atacaaagat aacaaataat tattcttttt attttcaagt
    14221 aaatttccat ctaaaactgt tccaggagtg atgactctta taacctctct tctaacaatt
    14281 ccttttgctg tagatgggtc ttccatttgt tcacctattg ctactttata tccattttca
    14341 actaattttg atatatatga attggctgag tggaaaggaa caccacacat aggagcacgt
    14401 tcttctaaac cacaagcttt tcctgtcaat gctatttcaa gagcttttga agctactaat
    14461 gcatcttcaa aaaacatttc ataaaaatct cccagtctaa aaaacagtat acagtcttta
    14521 tatctatttt ttacttcaag atactgtttc atcataggag tcaatttatt catatctatt
    14581 tgcattatca aacctccttc ttttctaatt aaatattata tcatagtaga tgattattat
    14641 taacacaata tataaaattt tgacgtataa ctaaaaacaa tccaaaatgt atactcacat
    14701 tttggattgc taagtttaat acttattaaa taaaaatata tataatttta caataaagat
    14761 attatttctt ttttaagttt aatgatgttt tcaaggtatc ttcatttaaa tttctactat
    14821 ctataacaaa ttctttcttt actattgatg gtttttttga aataacataa attctgtttg
    14881 atagcattat agcttcgtca atatcatgag ttataagaag tattgttgaa tttacttttt
    14941 tcttaacatc taaaagccat ttttgcatag atgttttagt taatgaatca agtgcgccaa
    15001 aaggctcgtc taaaagcatt atgtcattag aatttataaa agttctcaag aaattagctc
    15061 tctgtctcat tcctccagaa agttctgaag ggtatttatc ttcatatcca cttaatccaa
    15121 atacctcaaa ataaggtttt accattgacc tagctttatt ccttttttct ttttttaaaa
    15181 ctagtggtag tgatacatta tctattatag ttttatatgg caagagtaaa tctttttgat
    15241 acatatagct atatttacca tttatattca ctttgccact atcataatct gtaagtttag
    15301 ttataatatt aaaaatagta ctttttccac atccacttgg ccctaatata gaaacaaatt
    15361 caccttcata aacatcaata cttatatctt ttaaaacttc aatatcttta taagtttttg
    15421 atacattttc tatagatact tttacttttt tattttggta aaaattcatt tgtgtaagca
    15481 tcacttgcct tcatatcctt atctattaat ttatattctt ttaagaatga tgtatagttg
    15541 ttccatacac tatctttcat ttctccccat cgtggtgcat catctttgta tttgcttgct
    15601 aaatattctt gactctttaa ggctagtttt tcatctactt caggtgcatg ttttactaaa
    15661 atttttgatg cttcttctgg attttttatt gcatattcat aaccttttgt tgtagctttt
    15721 aagaattttt tagcaagctc tggattatct ttaagcacag tttcattcga aattataagt
    15781 ggtgtataat aatccaatct ctcatccaaa tcttttactg gtataaaatt taagtcgtat
    15841 ccaattaaat cagctttaac tgcatcccaa ccttcaaata tccatgcaaa atctacagtt
    15901 ttcatagctg caaagaaatc atcttgacca gtattgacta tttttaactt attaaaatca
    15961 gctccatttt tcttcataac tgctttaaaa acagcttctt ctgatggaga accccaacca
    16021 ccataagttt ttccttcaaa atctttagct gttgttatat tcttttcttt tggtgatgca
    16081 aatccagaag tattatgctg tataacagct gctacagctt ttattggaag tgggtcttca
    16141 ctagtctttg cataagtaac ctgctcttga taacttatac caaagtcagc ttttcctgtt
    16201 gctactaaag tctcggctcc actttcaggt ggttgtacta tttcaacatc taaaccttct
    16261 tctttataat aacctttatc aagtgctaca aataatccag tatgatttgt atttggtgtc
    16321 caatcaagta ccatagttac ttttttggaa gaagcatttt tatcattgtt tttatcttta
    16381 ttattatttg aagagcatcc agttattgtt gccaatgtaa aaacacttac taaacctaat
    16441 attcctaatt ttttaaaatt cataattgcc tccctcaaat acctttaatc tgttaatcta
    16501 tgtcttataa caattttttt tacaaactgt acaattccta caaacattaa actaaatata
    16561 actactaata tagtagatgc aaatacttta tcaagtgcat atgcactttt tgacctaagc
    16621 atataaacac caagtccttt cgttccacct agccattctg caacagttgc tgccattaca
    16681 gcatacgttg cagatatttt aagtccagaa aaaaacttat ccattgccat aggaaactta
    16741 aggtgaatga atgtattaac tttatttgag ttcatagtct taaataaatt taaataatct
    16801 ttatcaatat tctcaatacc atctacaaaa cttattaata taggaaaaaa acaagtaagc
    16861 attaccatca atacttttgg cattgttcca aatccaaacc atatcataaa aagtggtgct
    16921 ataattattg taggtatcat ctgagtaacc aacataatag gataaagaca tttttttatt
    16981 attgatacaa aatccatcaa tattccgatt accaatgata ttataattgc gactataaaa
    17041 ccaagtactg cttctcctat agtaaccaca gaatgcatgg ataaattttg ataatcttta
    17101 aaaaacacat taattatatc tactggtgtt ggtagtatat actgaggaac ttctaaaact
    17161 cctaccacag attgccaaag aataagtata aacaaaaatg ttgctattgg atatatacta
    17221 tctttgactt tatctatgat ttctaagttt ttcatctata gttagacctc cagctttttc
    17281 atctactcta gctactgtta tgactctttc acatccaaga ttaaaaggga ttttatgtac
    17341 cttttttaat aattcaaaaa cttcatcaaa atcaccttca acagtagtgc tcattgctcc
    17401 tatttcacat ttaagaccac ttttttcaat ttcagcaata caagcatcta ctactttata
    17461 catttcttct tcacctttat agggtcttag tggcattact gctatgtctg cgattaccat
    17521 ttaaatcact ccttttaaaa taattaatac ctactaggta tattttgttt tttattaaaa
    17581 aatttttttt actgaaaaga tattatattt aaataaaaga aaagtcttat ccaaaatagg
    17641 ataagactta tttttatctt aactggtctc cctacactgg cattatccaa acaggttcaa
    17701 agggtcgaga taaaatctcc tctcagcaca ttagctcccc gaaatatatt atatcttttc
    17761 tacttgatta tactacactt atcatatttt aacaaatttt ttatttatat tagatatttt
    17821 atctgtttaa aaatatctaa tataaattgc acattattat atcaaataaa ttatcttaaa
    17881 acgataattt ctcagtatct cctaaaacta gattaacgta attttctaat ttttgtttta
    17941 ctatttcaag catatttgga tatatatcaa ttccatccaa acattgtgct aaatttttat
    18001 ttttgttcat ttaagttaca atcattactg attaagcata gtttttatac tacactcatc
    18061 tttatataca aaaattcctt caataaattc cttaaaatta tattctaaat aagtgatttc
    18121 atagtcagat tcttgattaa tctctactac acagggttct tcttgtggtt cacaaccact
    18181 atagtaaaga aatatcattt cgtgcccacc agaaatggta ttatatatga ccatacctat
    18241 atagggttaa cacattctac tatccaaaaa cgactgccag cattgctaca taaggaatat
    18301 gttttttctg cgtcaatgac atatagactc tctaagtaga aaatgttaga tgccattcgt
    18361 tttgtatggt attatcaaat ttttttttag atttttattt cttcttatcc ttgtccatta
    18421 ttccttttat gacatagtat gcttcgtgag tgaataattg taaataaata atattcatta
    18481 agctaaaaat agacttctat ctggattttc aatatataaa ccttttagca gtattttctg
    18541 tgataatata aatattactc gtccttataa tcaaaatctt cagcaattgg ccttttatat
    18601 aatataatta ttttattcaa tttatttttg aaaaataaaa tattttttta atttttaaaa
    18661 tctttaatat gtagcttgaa aaacaaaacg ttttcctata tgatggtaat agtagaataa
    18721 aactaaatag gcaaaactag agaaatttag tgacgcaaag ctatagggac taagacttat
    18781 gaaatgagtt atgtcagcca gttgccaaag agatattttc tttttgtttt tatgaaagtt
    18841 ttttaggggg atatatgata tatgtttatg gataaaatgg atagatgtac acatatacta
    18901 actgcatata tatgcagttc acgcgattac tgcaatttta tagacacaca attaaatgat
    18961 tttatactag aatatggaga gaatgtggta gaatcttgtt tgcatcaggt gatggtattg
    19021 gtaagcagat ataattaaag aaattttatt ttctagaaaa ccctttttag ttttatctac
    19081 gttttataat tcattaaaaa actaattaaa ataagcttca ttatctattt ttcatgaagt
    19141 aaacagttga gtttatctca tatactgttt accccaatta cataattcta ataatataga
    19201 ttccaatgat ttaccacgtt tagtagacaa atattcaact tttggaggaa tagtaggata
    19261 agaaatcctc ttaataagtc catcgctttc aagatacttt aattcttgtg acagtatctt
    19321 atgacttata tgaacaactt ctttttttaa ttgactataa cgtattggct cttgttttaa
    19381 aatatgccat aaaataattg ccctccattt acttcctatc acagtcatgg taaaaccaat
    19441 tgaacaatta aaatcttttt gtctttcttc aatatttgga aattcttcaa aattattatc
    19501 tatatccatc tcaatcctct ctttcaaaat agtaagtatc taactaaaaa gtgcattctt
    19561 ttttaatgta ctaatttgta atataatctt gattgtagca tagaacctta cataatacta
    19621 cttttttaga tgtatatgtt taaattttaa tatagatata tgttatttta ttaattttat
    19681 ttaaacagat aataataaat aataatttat ctcaagtttt ctatgtacat aaactaatct
    19741 aatgtttaga aaggattata taatatgaat aaaaaatttc gtctttatca agttgactca
    19801 tttacaaaag aaaaatttac aggtaatcca gctggtgtaa ttacaaatgc tgatggctta
    19861 tctgaatcac aaatgcaaaa aatagcaaga gaattaaata attcagaaac tgcttttatt
    19921 ttcaataaat tggctaaaaa tcatcaagaa aattatgatg tacatatcag attttttaca
    19981 ccaactaatg aggttcctat atgtggtcat gctactattg ctgcccatta tgttagagca
    20041 gtagaaaacc aactcaataa caaaacagta tttcaaaaaa ctggtgctgg aatattacca
    20101 gttgatatta taaaaaaaga taatgattat gaaataatta tgacacaagg aaaaattgaa
    20161 gtatctccac cattacaaca aacctatcaa gaaagattac ttaatgcatt tggtatttta
    20221 aaggaagata ttcgtacaga ttgtcctatt gccatatcat ctacaggaca ttcaaaagtt
    20281 atgattggaa taaaggaatt atcaacatta aatagtttat ctccaaattt atatgaatta
    20341 tcgaaaataa gtcaagaaat aaaatgtaat ggatattata tttttacatt acatgataaa
    20401 gaaactctac aagttcatgg aagaatgttt gctcctgcaa ttggtatttc agaagaccct
    20461 gttacaggaa atgctaatgg acctttaggt gcgtaccttg tacattataa agtaatacct
    20521 atacccaaca cagagtttat atttgaagca gttcaaggag aagcaataaa acgtaaaggc
    20581 aagatgttag taaaagtaat tatttcagat aataagccag ttgaagtaaa aataattgga
    20641 aaagctgtaa ttgcattctc aacaaacata gaaattgaat tataggttcc ttctaagtaa
    20701 atttttgaac tttttaatac taatttaata acaaactaat ttatatacaa tcaaattaat
    20761 ttttttatta tttgttaata tatatttatc attatattat ataataatat agatagttac
    20821 tatatcttat aattagataa atcataatta tatattattg ttaaaactca attatataat
    20881 ttttaataat attattttag gaggtaaaaa tgataaacga tattaaaaat aaattcaaag
    20941 attacaaacc atatataaat ggatacaaga atatgaagag agcatctgtt ttaattccaa
    21001 tagtagaaat aaataacaca cactatatat tatttgaagt tcgctcaaaa aatttgaagc
    21061 atcaacctag cgaaatttct tttccaggtg gtaaaataga atcaggtgag actccatatg
    21121 aagctgttat tagagaaact tgtgaggaac ttggaacttt tagtgacaat atagaagtaa
    21181 tatcaagtct tgacctcctt ataacaccag taaagtttat catacatcca tacttaggat
    21241 atataaaaaa tattaataat ttaaatataa acaaggatga agttgaccat acttttttgg
    21301 ttcctataaa atatctttta gagaatcctc caaatactta tagtaatact gtaaaagttt
    21361 ttccagatga aaatctacca tatggttcaa ttccaaacca aaaagattat aaatttgaaa
    21421 gtggaagtta tcctattctg ttttataaat ataaagatta tgtaatttgg ggaatgacag
    21481 caaaaatact agaaaacttt ttaaattttt tatcagaata acacttataa taaatcatta
    21541 aaacttttac ataagaaatt cataaaagtt tctaaattta aaatttaaca gttctaaaat
    21601 atcaagaact atattttgta gcactttttt attttattat tgtttgaata tttgatagtt
    21661 ttcaatgcaa tttcaacata aatactatgt attagtgtat actgttgctg aattaaagta
    21721 tgataatcta tttaataaag tatgcagtaa atgattttaa tgtgtcttta tggtaaaatt
    21781 actaataagt aataaaatta attaaaatat atggaggtgc ttgtaatgtt tgctgtagga
    21841 tttgttgtta taattgcact tgcctttttc atattaagtg tactattatt taatggaaaa
    21901 gccagtatgc tactagcagg ttataactct atgagtaaga aagaaaaagc acgatacgat
    21961 aaaaaaaaat tatgtagaag ttctagcatt gttactttta ttgtctctat aatgctattt
    22021 attatggcat ttttgggata tagaatagag agtggtcaaa tggacgaaaa aaaaatggtt
    22081 accttttcta taatatttat aatagtcata ctttccagtg tattttttaa tatcctctac
    22141 tctaacaaaa aatgtaaaaa ataaatagtt gtaagttgag cagttaatta taaaagattg
    22201 actgcttttt cct
//
LOCUS       DAFNNB010000052        22178 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0052, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000052 DAFNNB010000000
VERSION     DAFNNB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 22178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0052"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            55..930
                     /locus_tag="L3M60_001699"
     CDS             55..930
                     /locus_tag="L3M60_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HBN6226374.1"
                     /translation="MKKRLIIILSLVLVLSSVLTIFAYSNIKYNNKLIYSNMIDKRTQ
                     NSVEEILKENKINEKDIDTFIKAVNNYNKLQVKILQNNINISKSGYSSINAKQVPYNL
                     EKLQDNWMKKFPDYMDVNCRITAFRLFKDFINSNNKFTGDSIDLNVDLDTIMNNKDAK
                     FSTKDVEKFINFFSAIPAKDTSDISKIAEQIKNEWKKRKISFKNNKNISIINGFLRYP
                     ETKNVFIGHTGICIKTKNGILFVEKYGPTSPYQVTKFKNKKDVKNYMFNRLKMSESEI
                     ELPDPVIMENDKLMK"
     gene            complement(1246..2025)
                     /gene="hisJ"
                     /locus_tag="L3M60_001700"
     CDS             complement(1246..2025)
                     /gene="hisJ"
                     /locus_tag="L3M60_001700"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ"
                     /protein_id="HBN6226375.1"
                     /translation="MKDGHIHSPYCPHGSKDDFEKYVQRAMDIGITEMTFTEHFPYAN
                     GFRDPAPENDSCMSIEDLPKYFNDVKKLKEKYEGKIKINVGSEVDFIEGYEEGIKANL
                     NKYGEELEDSLLSVHIIKIDDDYYCVDYSIEEFQNLIDKLGSIEAVYNKYYETLIKAV
                     NSDLGIHKPKRIGHLNLVRKFNQVFPYDYKGNTILEDLIKLIKEKGYELDYNVSGNRK
                     EYCKEPYIDGYLLELVKKYDIPLVLGSDSHCAEEINKYDIV"
     gene            complement(2294..2644)
                     /locus_tag="L3M60_001701"
     CDS             complement(2294..2644)
                     /locus_tag="L3M60_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter"
                     /protein_id="HBN6226376.1"
                     /translation="MIFDREIVRYHHGHHDHDHCHEHTHGDICHEHPHDHAHDHDHEH
                     SHEESSESKDEKTLKILLVHWINHNETHEEGFREWVEKARAIGKEETAKSIEKAIEYM
                     EEANKMLLEAKKHM"
     gene            complement(2660..3076)
                     /locus_tag="L3M60_001702"
     CDS             complement(2660..3076)
                     /locus_tag="L3M60_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3842 family protein"
                     /protein_id="HBN6226377.1"
                     /translation="MIIAVIDGMGGGIGAQIVSSLREELPTYVEIYALGTNSIATSSM
                     MKAHANKGATGENAIVVSAKKANIIVAPISVIIPNSMMGEVTCNISEAIADSEALKIL
                     LPIMPENVELVGLEGKPLALLVKDSVNLIKKEFNIK"
     gene            complement(3095..3286)
                     /locus_tag="L3M60_001703"
     CDS             complement(3095..3286)
                     /locus_tag="L3M60_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CooT family nickel-binding protein"
                     /protein_id="HBN6226378.1"
                     /translation="MCESSAFICKSNNELEKVMENVVNIDPCDGKIYLTDLLGEQKII
                     DGIIKEIRLMDHKIIIQEN"
     gene            complement(3437..4252)
                     /locus_tag="L3M60_001704"
     CDS             complement(3437..4252)
                     /locus_tag="L3M60_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HBN6226379.1"
                     /translation="MNQWRVIHNKSYEGAMNMAIDEAIFTAYKKGHNKPTLRFYTWEP
                     ACLSIGYFQKLEDEIDLDKCRCMNIDYTRRITGGRAVLHDNELTYSIIIGEDNPLIDK
                     SINLSYRYISEGLVKGLNLSGIETDNLNRGERISRENLSAACFNAHASYEVTINNKKV
                     IGSAQSRKDGVLLQHGSIILDFDVEKLFKLIKTKTPELKERAMKFTAKKASGIENEIG
                     RKIDIDILQKNIVKGLAEQFNVEFVEGDLTDYEKQLAKELYEKYKNEEYNKKR"
     gene            complement(4299..5237)
                     /locus_tag="L3M60_001705"
     CDS             complement(4299..5237)
                     /locus_tag="L3M60_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6226380.1"
                     /translation="MIRLSVGTAIELGILNKKSDIPPTTAYIMIGEKCINKCSFCSQS
                     IESSTRKDKLSRVVWPEFSKEEILDALKAYKGKNIKRICIQSMASEEAHNSVLDFINY
                     ISGKIDMPISLSAKLENDEQINKFFSAGVDKIGIAIDAANKELYEKIKGNNYNEKLKF
                     ITEMSKSYPNKISTHIIVGMGESHEDIYNLYTYLKGNDITISLFAFTPVRGTKMEKIN
                     QPNIESYRRVQLMSYMINKGYSKEYFTFKNGYLDSIKLDNDIVKDINKGYPFEIRGCK
                     DCNRPYYNERPGSTIYNYSRPLNQSEIDLAIREINL"
     gene            complement(5234..6106)
                     /locus_tag="L3M60_001706"
     CDS             complement(5234..6106)
                     /locus_tag="L3M60_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6226381.1"
                     /translation="MNKNLSEKVKVAFDIKLKNFGNKIEFAYPNQTLALSTTNNKCSL
                     KCAHCNGHYLNNMVPIEDYEEKVQSRNITSFLLSGGCSYDGDVPMSTHIQTIKRLKEQ
                     GYRLNAHLGLMDKESIIELCKYLDIVSFDLVFDKETIREVYKMEKSKDDYIEVYNTIR
                     ENTEVAPHICIGLKGGQIKGEYEIIEYLQKNPPNKLTFIILIPTKGTEYENVEPPELE
                     RVADILCEARINLPYTEINLGCMRPRGVYRKELDQLSIMCGVNKIVLPSRSAKNKAIE
                     MNMTITECKECCVL"
     gene            complement(6131..8059)
                     /locus_tag="L3M60_001707"
     CDS             complement(6131..8059)
                     /locus_tag="L3M60_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4445 domain-containing protein"
                     /protein_id="HBN6226382.1"
                     /translation="MIKVSFTPNNKEVYCNEGDILLEVARNADIFIDAPCNGNVSCGK
                     CKVKLLNGKVDTEKTRHITDDEWEQGYILACCTKVISDIEIEVPSKVSSSMHGMKIEG
                     SNKKEDREIFERAKKIIEEHNLQFKTNIKKKYIEMEEPNLDDNISDVDRLERYVRNNL
                     GYNEIDFRLDILRKMPTIFRKSDFKVTITYVQKQKKLTIINIEEGNKENSLYGVAIDI
                     GTTSVVVCLVDLYSKEVVDKASSGNAQIKYGADVINRIIYSTKKNGLETLHKAIVEET
                     INPLLKTIYERNGIDKEDVVTLVAAGNTTMTSLFLGVYTDFLRQEPYIPPFLKSPKLM
                     GENVGLFVNDSAYVYLAPSVASYVGGDITAGVLSAGIWSSEENVLFIDLGTNGEIVFG
                     NKDYMMSCACSAGPAFEGGGISCGMRASAGAIEKVIIDKETLEPTLKIIDECAPVGIC
                     GSGIIDLICQMITKGVIDRRGKIYRDLDNKRVRFNEHEIGEYVLAFKEEFDLENDIVV
                     NEVDIDNFIRAKGAIYSGAYTLVDSLGMDFSILDRVYIAGGIGNNLDIENSIIIGLLP
                     DIDREKFTYIGNSSLVGSYLALISKDAKNKLEEIGNQITYVELSVYPSYMDEFISACF
                     LPHTNIEQFPTAKKLLEE"
     gene            complement(8171..8548)
                     /gene="gcvH"
                     /locus_tag="L3M60_001708"
     CDS             complement(8171..8548)
                     /gene="gcvH"
                     /locus_tag="L3M60_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBN6226383.1"
                     /translation="MKLLPELKYSKDHEWVKVIDGDVVYIGITDYAQDQLGEILFVET
                     PEVEDTVTKGVDFGVVESSKVASDLISPVNGEVLEVNEKLEDEPECINEDPYENWILK
                     VKLADVAELDTLLSDKEYEAGLE"
     gene            complement(8635..10761)
                     /gene="cdhC"
                     /locus_tag="L3M60_001709"
     CDS             complement(8635..10761)
                     /gene="cdhC"
                     /locus_tag="L3M60_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/CO-methylating acetyl-CoA
                     synthase complex subunit beta"
                     /protein_id="HBN6226384.1"
                     /translation="MNLYNIIFTGSEQALGAAQAMLAEAIEKNGKEHKVAFPDTAYSL
                     PCIYAATGQKMNTLGDLEGALEVVKSLINRTHLLEHAFNAGLATALAAEVIEALKYST
                     MDAPYSEPCAGHITDPIIRSLGVPLVTGDIPGVAVVLGECPDAESAAKVIKDYQSKGL
                     LTFLVGKVIDQAIEAGVKMGLELRVIPLGYDVTSVIHVVSVAVRAALIFGGLTPGDLN
                     GLLEYTANRVPAFVNAFGPLSELVVSAGAGAIALGFPVITDQTVLEVPMNLLTQKDYD
                     KIVATSLEARGIKIKVTEIPIPVSFAAAFEGERIRKSDMFAEFGGNRTEAWELVVKKE
                     ATEVEDHKIEIIGPNIDEVDADGVLRLPLAVIVKIAGKNMQEDFEPVLERRFHYFLNY
                     IEGVMHVGQRDMAWVRISKDAFDKGFRLEHIGEVLYAKMLDEFESVVDKCEITIITDA
                     EKVSELKGEAIAKYNARDERLASLVDESVDTFYSCNLCQSFAPAHVCVVTPERLGLCG
                     AVSWLDAKATKELDPTGPCQPIEKGECLDDRTGVWNSVNETVNQISQGAVESVTLYSI
                     LEDPMTSCGCFECICGIMPEANGFVVVNREFASVTPVGMTFGELASMTGGGVQTPGFM
                     GHGRHFISSKKFAYAEGGPERIVWMPKELKDYVADKLNATVKEMTGIENFCDMVCDET
                     IADDSEGVLAFLEEKGHPALAMESVM"
     gene            complement(10818..11624)
                     /locus_tag="L3M60_001710"
     CDS             complement(10818..11624)
                     /locus_tag="L3M60_001710"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBN6226385.1"
                     /translation="MEKFMIIGERIHCISPSIRKALAERDPAPILKRAKEQLEAGAHY
                     IDFNIGPAERDGEEIMTWGIKLLQSEFNNVPIALDTANKKAIEAGLKVYDRTNAKPII
                     NSADAGSRFDLIDIAAEYEAMVIGLCAKEGIPRDNDERMAYCQEILEKGLMLGMEPTD
                     ILFDPLCLVIKGMQEKQVEVLEAIKMMTEMGLLTTGGLSNVSNGCPKHVRPVLDSAFL
                     AMAMANGFSSAIMNPCDPELMKTVKSCDIINGASLYADSFLELNEGGFAF"
     gene            complement(11674..13041)
                     /locus_tag="L3M60_001711"
     CDS             complement(11674..13041)
                     /locus_tag="L3M60_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HBN6226386.1"
                     /translation="MALKALDIFKLTPKKNCKDCGFPTCMAFSMKVASGAVEVGKCPH
                     MSDDAIAKLSEATAPLMKALKVGAGASEYELGGETVLFRHEKTLVSRNRYAVSFCTCM
                     SDEAVDSKIANMKKVDYVRIGEQMKVEMAVLEYCGDKDAYLKLIDKIKGSGLEVAYIL
                     ACDDAQVVKEAVEVLKDARPMVYGATKENYKDMIEVVKGASLPLGVKAGSLEELYETV
                     ELIQAAGYKELVLDVTGENIKDTYTNAIQVRRIALKEQDRTFGYPSIVFANRLSNSNP
                     MMEVALSSIFTIKYGSIIVIDDISYAKALPLFALRQNIYTDPQRPMRVEPKIYPINNP
                     DENSPVLVTVDFALTYFIVAGDIERSKVPVWLVIPDAGGYSVLTSWAAGKFGGNSISA
                     FIKESKVEEVTNCKDLIIPGKVAVLKGDIEDNLPGWNVVIGPEESMELPKFLKGYQEK
                     ACQTN"
     gene            complement(13072..14016)
                     /locus_tag="L3M60_001712"
     CDS             complement(13072..14016)
                     /locus_tag="L3M60_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit delta"
                     /protein_id="HBN6226387.1"
                     /translation="MTFKMSTQKYSGKISEVEVGIGEKAIKLGGENVLPFYSFDGEVG
                     NPPKIGIQISDVYPESWTDSYKELYKDVAKCPVEWAKYVEANTQADFICLKFDGSDPN
                     GLDKSVDECADVAKAVIEAIKLPLVVAGSGNHEKDGKLFEKLAQTLDGHNCLFMSAVE
                     DNYKGVGASAGMAYSHKVGAESSVDINLAKQLNVLLTQLGVKGENIVMNVGCSAVGYG
                     YEYVASTMDRIRLAAFGQNDKTLQMPIITPVAFEVGHVKEAIAPIEDEPDWGCPEERT
                     IAMEVSTAASVLVGGSNAVILRHPKSIETIKELVNALA"
     gene            complement(14036..14806)
                     /locus_tag="L3M60_001713"
     CDS             complement(14036..14806)
                     /locus_tag="L3M60_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6226388.1"
                     /translation="MGYNIAVAGKGGTGKTSLTGLLIDYLVKDKKGPVLVVDADANAN
                     INEVLGIEVEATIGEIREEVNQREKLGNAFPGGMTKAQYLQFRLNSIIEEGEGYDLLV
                     MGRSEGEGCYCFVNGILREQVNKISGHYKYLVMDNEAGMEHLSRKVTRHVDTLLLVSD
                     CSRRSIQAVARIRDLAEELKLSVGRILLIVNKVPNGVMNDGVKEEIEKHNLELIGVVP
                     MDELIYEYDSTGIPLVNLPEDSKSKVAMKEIFAKLELK"
     gene            complement(14862..16247)
                     /gene="lpdA"
                     /locus_tag="L3M60_001714"
     CDS             complement(14862..16247)
                     /gene="lpdA"
                     /locus_tag="L3M60_001714"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBN6226389.1"
                     /translation="MKIVVVGGGPGGYVAAIKASMLGADVTVIEKRRVGGTCLNAGCI
                     PTKALLASSGVLNTVKEAKDFGIEIDGTVKPNFTAIMERKNKVVNQLISGIEFLFEKR
                     GVNLVNGFGKLIDKNTIEVTKDDGTVETIKADKIILANGSVPVVPRMFPYDGKVVITS
                     DEVLGLEEIPESMLIVGGGVIGCEIGQFFRALGTEVTIVEMVDQILLNEDKDVAKQLL
                     RQFKKDKIKVITGIGVQTCEVVDGKAVATLSNGKVIEAQYALVCVGRRPNLDNSGVED
                     IGIEMERGKVVVNEHLETNVEGIYAIGDIIDTPFLAHVASKEGIVAVENALGKTKVVD
                     YRAIPRCVYTEPEVAGVGKTEKQLEAEGVEYNVGQFDFRGLGKAQAIGHFQGFVKVIA
                     DKETDKIIGAAVVGPHATDLLTELSLAVHLGLTVEQVGDAIHPHPSLSEGLMEALHDV
                     HGECVHSVPKL"
     gene            complement(16291..17172)
                     /locus_tag="L3M60_001715"
     CDS             complement(16291..17172)
                     /locus_tag="L3M60_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBN6226390.1"
                     /translation="MSLLRETLESGKFAVTTEMAPPKGTDLSHLIECAKPLVGRVHAA
                     NVTDFQSAVMRATSLATCKLLKDAGLEPVIQITGRDRNRIAIQGEMLSAGVFGINNLL
                     ALTGDHTSVGDHPQAKGVFDLDSVGILQTAETLMAGTDMAGNKLKGSPDFYLGASVTP
                     EYSPIEVQLLKMKKKIKAGAKFFQTQALYDINTMRKFRELTRDMDCKVLAGIVPLKSP
                     GMAKFMTANVPGIFVPDEQIERLRVAGKENWVSEGIKMAGELIKQLKEEDLCDGVHIM
                     AIGAEENIPAILDAAGL"
     gene            complement(17208..17882)
                     /locus_tag="L3M60_001716"
     CDS             complement(17208..17882)
                     /locus_tag="L3M60_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HBN6226391.1"
                     /translation="MIISENKPLEEVLGYLKDFDKLVLVGCNQCAATCKSGGEEEVLK
                     MKETLEGEGKKVLGYVMLDPACNLLKSKKDLKALKEETKEADAVLSLACGDGTQTIVK
                     NLKDKPVYPANNTLFIGEVQRVGEYEEACKACGDCELGWTGGICPVTMCAKGLMNGAC
                     GGAKNGKCEVNAENDCAWIKIYERLEAIGQLDNLVEIRPPKDYSKQNNPRSLSAKKKK
                     EAAANS"
     gene            complement(17917..18789)
                     /locus_tag="L3M60_001717"
     CDS             complement(17917..18789)
                     /locus_tag="L3M60_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylene-tetrahydrofolate
                     dehydrogenase/5,10-methylene-tetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HBN6226392.1"
                     /translation="MEGMSTKGQIIKGKPVADKISEELIKEVDLLVKEGINPKLTIVR
                     VGARSDDLSYERGALKRCQNIGITTEVLELAEDITQDEYIDVLKRVNDDKNVNGILCF
                     RPLPKHLNEEVIKYVIAPEKDVDCFSPINSAKVMEGDKSGFPPCTPTAVVEILKHYNV
                     DLKGSKVTVLGRSMVVGKPVSMLLLSEHATVTICHSKTKNLSSVAAEADVLIAAIGRA
                     KMVDESFVKDGAVVIDVGINVDEEGNLCGDVDTNAVLNKVSMITPVPAGVGSVTTSIL
                     AKHVVKACKLQNNK"
     gene            complement(18881..19513)
                     /locus_tag="L3M60_001718"
     CDS             complement(18881..19513)
                     /locus_tag="L3M60_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HBN6226393.1"
                     /translation="MKIADKTCVDFVEVLSSKEAVPGGGGAAALVGAIGMALGSMVCN
                     LTIGKKKYAEYEESVKEILSKAGKLEKDLLKMIDDDAECFLPLSKAYGLPKETEEEKR
                     IKAETMEKALKVACEVPLNIVRVCYEAIKLHEDLVDKGSRLAISDVGVGVQCLRAAIL
                     GGQLNVVININSIQDKEYVDKVKTEVDKLVEDGVKICDEVYTKVEKALGK"
     gene            complement(19598..21274)
                     /locus_tag="L3M60_001719"
     CDS             complement(19598..21274)
                     /locus_tag="L3M60_001719"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HBN6226394.1"
                     /translation="MGFKSDIEIAQEAKPQDIREVAKKLGLGEDDVELYGKYKAKVDY
                     NLLKRETGKKAKLILTTAINPTPAGEGKTTTTIGVADAFAKLDKNVLVALREPSLGPV
                     FGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIGAANNLLAAMLDNHIHQGNALRIDPK
                     KITWRRCVDMNDRQLRNIVDGMGKKGDGAVRQDGFDITVASEIMAAFCLASDISDLKE
                     RLGNIIVGYSYEGEPVTARQLKANGAMAALLKDALKPNLVQTLEGTPSFVHGGPFANI
                     AHGCNSVIATRMAMHFADYVITEAGFGADLGAEKFLDIKCRMANLKPDAVIIVATVRA
                     LKYNGGVAKADLNEENLEALEKGLPNLLKHVENITQVYGLPAVVAINRFPLDTEAELK
                     LVEDKCKALGVNVALSEVWAKGGEGGIAVAKEVLRLLDEEENNFKFCYEDDLSIKDKI
                     NAIATKIYGADGVDYTPEADKEIANLERLGFTKVPVCMAKTQYSLTDDQTKLGRPTGF
                     RITVRQATISAGAGFIVALTGEIMKMPGLPKVPAAEKIDVDENGVIAGLF"
     gene            complement(21523..>22178)
                     /locus_tag="L3M60_001720"
     CDS             complement(21523..>22178)
                     /locus_tag="L3M60_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HBN6226395.1"
                     /translation="ALALGFPKEVYESIVPISEMKKLVSDRTAASVGSFGKMFKMNPK
                     VDDIPENFCKEYNGVRLLTLGTVDSGGTGCVCPEHVLLKRLCSHLILQNKDVVVMDME
                     AGIEHLGRGTAQGVDAFIVVVEPGERSLQTYRKVKKLGHDIGVNKVFVVGNKIRNKED
                     EEFIIQNLEDGESLGFIYYNQDVIDSDRANQSPYDSSETTKEQIKAIKDKLMSLKDK"
BASE COUNT         6638 a         4508 c         2537 g         8495 t
ORIGIN      
        1 gtactatata ctataaatat gtcgacacaa taaatttaaa aaggagaact caaaatgaaa
       61 aaaagattaa taattatatt atctcttgtc ttagtactat caagtgtact tacgatattt
      121 gcatattcaa atattaaata taataataaa ttgatatatt ctaatatgat agataaaaga
      181 actcaaaata gtgtagagga aattctaaaa gaaaataaaa ttaatgaaaa agacatagat
      241 acatttatta aagcagtcaa caattataat aaattgcaag ttaaaatact tcaaaataat
      301 ataaatatat ctaaatctgg atattcttca atcaatgcaa aacaagttcc atataaccta
      361 gaaaaattgc aagataattg gatgaaaaaa ttccctgatt atatggatgt aaattgtaga
      421 ataactgctt ttagactttt taaagatttt ataaattcaa ataataaatt tactggagac
      481 tctattgatt taaatgtcga cttggacact ataatgaata ataaagatgc taaatttagc
      541 accaaagatg tagaaaaatt tatcaacttc ttttctgcaa taccagctaa agatacaagt
      601 gatatatcta aaattgcaga acaaataaaa aatgagtgga aaaaaagaaa aatatctttt
      661 aaaaataata aaaatatatc tattataaat ggatttcttc gttatccaga aactaaaaac
      721 gtatttatag gtcatacagg catatgtatt aaaactaaaa atggcattct atttgtagaa
      781 aaatacggcc ctacatcacc ttatcaagtt actaaattta aaaacaagaa agatgtaaaa
      841 aattatatgt ttaatagatt gaagatgtca gaaagtgaaa ttgaattgcc tgaccctgta
      901 ataatggaaa acgataaact tatgaaataa taactttaaa tgcaaaatag tacttataat
      961 tactaagtca atataataga gaatatctat agatttgtgt aaatacaagc ttatctacaa
     1021 tcttatataa gaatgtttat ataaataaga tagattaatt ctaaatccta ttattctatt
     1081 gaattttatt attattattt tacttagatt ctattgttat tttacttaga actcattatt
     1141 attttattta agttctatta ctatttttaa tcatataaat ttacaaaaga ctgtagataa
     1201 atttatttta ttcaaactta tctacagtct tttatgcaaa tatatctaaa caatatcgta
     1261 tttatttatt tcctctgcac agtgagaatc agagcctaaa actaatggaa tatcgtattt
     1321 ttttaccaac tctaataagt atccatctat ataaggctct ttgcagtact cttttctatt
     1381 tccagaaaca ttgtaatcaa gttcataacc tttttcttta attaacttta ttaaatcttc
     1441 aagaatggta tttcctttat aatcgtatgg aaatacctga ttaaactttc taactaaatt
     1501 taaatgacct attctttttg gtttatgtat acctaaatct gaatttacag cttttatcag
     1561 tgtttcataa tacttattat aaacagcttc aatactacct agcttgtcga ttaaattttg
     1621 aaattcttct atactgtaat ctacacaata ataatcatca tcaatcttaa ttatatgtac
     1681 tgataaaaga gaatcctcta attcttctcc atatttattt aaattcgcct ttatcccttc
     1741 ttcgtatcct tctataaaat ccacttctga cccaacattt atcttgattt taccctcata
     1801 cttttctttc aacttcttga catcattaaa atactttggc aaatcttcaa tactcataca
     1861 actatcattt tcaggagcag ggtctctaaa tccatttgca tatggaaaat gttccgtaaa
     1921 ggtcatttct gttattccaa tatccattgc tctttgtacg tatttttcaa aatcatcctt
     1981 actcccatga ggacaataag gtgaatgtat atgaccatct ttcataaata attcactcct
     2041 tttttaaaga aaaaatctta tataaataca taaatttttt aacttcaaaa caatattttt
     2101 aattatacta attcttatta tatctaatct tggagcaatt ttaaacagcc tttaaagtat
     2161 atttttatca tttcaacaat gaaagagagg cttatatcta ttttgagaca atccattaga
     2221 taagaaattt aataattaag catcaaaaaa cataaaaatc gcttatatca aacgtataag
     2281 cgattaatta tatttacata tgttttttag cctctaacaa cattttatta gcttcttcca
     2341 tatactctat agccttttca atactctttg ctgtttcttc ttttccaata gctctagcct
     2401 tttcaaccca ctctctaaat ccttcttcat gagtttcatt atgatttatc caatgtacca
     2461 aaagtatttt taaagttttt tcatccttag actctgatga ttcctcatga ctatgttcgt
     2521 ggtcatggtc atgagcatgg tcatgtggat gttcatgaca tatatctcca tgagtatgct
     2581 cgtggcaatg gtcatggtca tgatgtccat gatgatatct tacaatctct ctatcaaaaa
     2641 tcattagtta ttccccctgt tattttatat taaactcttt ttttataaga tttacagaat
     2701 cttttacaag tagtgctaat ggtttacctt ctaatccaac taactccaca ttttctggca
     2761 taataggtaa aagtatctta agtgcctcac tatctgcaat agcttcactt atattacaag
     2821 taacttctcc catcatagaa ttaggtatta ttacagaaat aggtgcaact attatatttg
     2881 cttttttagc tgatactact atagcatttt cccctgtagc tcctttatta gcatgagcct
     2941 tcatcataga actagttgct atagaattag tccctagtgc ataaatttct acatatgtag
     3001 gtaactcttc tctcaaagaa gaaactatct gggctccaat acctccaccc attccatcta
     3061 tcactgctat tatcatatga aattccccta ttaattaatt ttcttgtatt attatcttat
     3121 ggtccatcaa tcttatttct ttaattatcc catctattat cttttgctct cctaataaat
     3181 ccgttaaata aatctttcca tcacaagggt ctatattaac cacattttcc ataacctttt
     3241 ctaactcatt attactttta caaatgaaag ctgatgattc acacatataa atctcctcct
     3301 aatatgattt ttattaaaaa tcaaaaaaag atttccttag gtatttcaat acaccaaaga
     3361 aaccttcatg atttcagttt ttgtatatta ttatacctta agtatagcac tttaatttat
     3421 ataaagtcaa ttaaagttaa cgctttttat tatactcttc attcttgtat ttttcatata
     3481 gttctttagc taattgcttc tcgtaatcag ttaaatcacc ctccacaaac tcaacattaa
     3541 attgttcagc taatcccttt actatgtttt tttgtagaat atctatgtct atttttctgc
     3601 caatttcatt ttctattcca cttgcttttt tagctgtaaa tttcatagcc ctttctttca
     3661 gttctggtgt cttcgttttt attaatttaa acaatttttc tacatcaaaa tccagtatta
     3721 ttgaaccatg ttgtaataaa acaccatctt ttctgctttg agcacttcca attactttct
     3781 tattattaat ggtaacttca tatgatgcat gtgcattgaa acacgcagct gataaatttt
     3841 ccctacttat tctctcgcct ctattcaaat tatctgtttc tataccactt aaattcagtc
     3901 ctttaactag accttcactt atatatctat aagacaggtt gatgctctta tcaattaaag
     3961 gattatcttc tcctataatt atactataag ttagctcatt atcatgtaat acagcccttc
     4021 cacctgtaat tcttctagta tagtctatat tcatacatct acacttgtcc aaatctatct
     4081 catcttccaa tttctgaaaa tatcctatac tcagacaagc tggttcccat gtataaaatc
     4141 taagagttgg cttattatgt cccttcttat atgcagtaaa tatagcttca tcaattgcca
     4201 tattcattgc tccttcatat gatttattat gtataactct ccactgattc atgattatta
     4261 cgcccccatt tttagtataa atatatccta gaaatcctct atagatttat ttctcttatt
     4321 gctaaatcta tctcactttg atttaaaggt cttgaatagt tatatatagt actacctggt
     4381 ctttcattat agtatggtct attacaatcc ttacaacctc ttatttcaaa aggataacct
     4441 ttgtttatgt cttttacaat atcattgtca agctttatgc tatctaagta tccattttta
     4501 aacgtaaaat actcttttga ataaccttta tttatcatat aagacattaa ctgtaccctt
     4561 ctatagcttt ctatatttgg ttgatttatc ttttccattt tagttcctct aactggagta
     4621 aatgcaaata aactaatcgt tatgtcattt ccctttaaat aagtatataa attgtatata
     4681 tcttcatgac tttcacccat accaactatt atatgtgtac ttattttatt gggatatgat
     4741 tttgacattt cagttataaa ttttaacttt tcattataat tattgccttt tattttttca
     4801 tatagttctt tatttgctgc atctattgct attcctattt tatctactcc tgctgaaaaa
     4861 aacttattta tctgttcatc attttctaat tttgcagata gtgaaattgg catgtctatc
     4921 ttgccactta tatagtttat aaaatctaaa acagagttgt gtgcttcttc acttgccatt
     4981 gattgaatac atattctctt tatatttttt cctttataag cttttaacgc atctaatatt
     5041 tcctcttttg aaaactctgg ccaaactact ctggacaatt tatcttttct agtactactt
     5101 tctattgatt gggagcaaaa actacattta tttatacatt tttctccaat cattatatat
     5161 gctgtagtag gaggtatatc gctttttttg tttaatattc caagttctat tgctgttcca
     5221 actgatagtc taatcataaa acgcaacact ccttacattc agttattgtc atattcattt
     5281 ctatagcttt attcttggca gaccttgatg gtaatactat tttattgact ccacacatta
     5341 tagataactg gtctagctct tttctataca ctcctcttgg tctcatacac cctagattaa
     5401 tttcagtata tggtaaatta attctagctt cacataaaat atctgctacc ctttctaatt
     5461 caggaggttc tacattttca tactcagttc cttttgttgg aataaggatt ataaatgtaa
     5521 gtttattagg aggatttttc tgtaggtatt cgataatttc atattcacct tttatctgtc
     5581 ctccttttaa accaatacat atatgtggtg ctacttctgt attttctctt attgtattat
     5641 aaacttctat ataatcatct ttgctctttt ccatcttata aacttctctt attgtttctt
     5701 tatcaaatac taaatcaaaa gaaactatat ctaaatactt acacaattct ataatgctct
     5761 ctttatccat aagtcctaaa tgagcattca atctgtaacc ttgctccttt aatcttttaa
     5821 tagtctgaat atgagtactc attgggacat caccatcata gctacatcca ccacttaata
     5881 aaaaactagt tatatttctt gattgtactt tttcctcata atcctctatt ggtaccatat
     5941 tgtttaaata gtgaccattg caatgagcac attttaaact acatttattg tttgtggtac
     6001 taagagctag tgtctgatta ggataagcaa actctatttt atttccaaaa ttttttaatt
     6061 taatatcaaa tgctactttc actttttcag atagattttt attcatagtt tattccttct
     6121 taagttcatt ttactcttct aataatttct ttgctgttgg aaactgttct atattcgtat
     6181 gtggtaagaa acatgcagaa ataaattcat ccatatagct tggatataca ctaagttcaa
     6241 cataagttat ttgattacct atttcttcaa gtttattttt tgcatcttta cttatgagag
     6301 ctaaataaga accaacaagt gaactatttc ctatataagt aaatttttct ctatcaatat
     6361 caggaagtaa acctataatt attgagtttt ctatgtctag gttatttcct ataccacctg
     6421 ctatataaac cctatctaat atactaaaat ccatacctaa actgtctaca agagtgtatg
     6481 cacctgaata tatagctcct ttagctctaa tgaaattatc tatgtcaact tcattgacta
     6541 ctatatcatt ttctaaatca aattcttctt taaatgctaa tacatactct cctatctcat
     6601 gctcattaaa tctaactctc ttgttatcta aatccctgta tattttacct cttctatcta
     6661 ttacaccttt agtaatcatt tgacagatta aatcaattat accagaacca catataccaa
     6721 ctggtgcaca ttcatctata atttttaatg ttggctctaa agtttcttta tcaataatta
     6781 ctttctctat tgctcctgct gatgccctca ttccacagct tatacctcca ccttcaaagg
     6841 caggacctgc tgaacatgca caactcatca tataatcttt atttccaaat actatctctc
     6901 cattagttcc taaatctata aataaaacat tttcttcact tgaccatata cctgctgata
     6961 atacacctgc tgttatatct cctcctacat aacttgcaac agatggagct aagtacacat
     7021 atgcactgtc atttacaaaa agaccaacat tttctcccat aagttttgga gattttaaaa
     7081 atggtggtat gtaaggctct tgtcttaaga agtctgtata tacacccaag aataaacttg
     7141 tcatagttgt attcccagca gccactaatg taactacatc ctccttatct attccatttc
     7201 tctcatatat tgtttttagc agtggattta tagtttcctc aactatggct ttgtgaagtg
     7261 tctctaatcc atttttctta gttgaatata taattctatt tataacatct gctccgtatt
     7321 ttatctgtgc atttccagaa gatgctttat ctactacctc ttttgagtat aggtctacta
     7381 agcaaacaac tactgaagtt gtaccaatat ctattgcaac tccatataag gaattttctt
     7441 tatttccttc ttctatattt atgattgtaa gtttcttttg tttttgaaca tatgttattg
     7501 taaccttaaa atcactcttt ctaaatatgg ttggcatctt tctaagtata tccaatctaa
     7561 aatctatttc attgtaccct aagttatttc taacatatct ttccaatcta tctacgtcgc
     7621 ttatattatc atctaaattt ggttcttcca tttctatata tttctttttt atatttgttt
     7681 taaattgaag gttatgttct tcaattattt ttttagctct ttcaaatatt tctctatcct
     7741 cttttttatt actaccttct atcttcatac catgcataga agatgatact ttagaaggaa
     7801 cttcaatttc tatatctgat attacctttg tacaacaagc tagtatataa ccttgctccc
     7861 actcatcatc tgttatatgt cttgtttttt ctgtatcaac ttttccattc aataatttta
     7921 ctttacactt accacaagac acgtttccat tacagggagc atctatgaat atatctgcat
     7981 ttcttgcaac ctctagtaat atatccccct cattacaata aacctcttta ttgtttggtg
     8041 taaaactcac ttttatcatg ttatccctcc caatttgttc tgtatatgat aaaaggaaag
     8101 tatagctaaa tttgcgtgca ctcaaatttt actatacttt ccccaattat aataatatcc
     8161 taatctttaa ttattctaat ccagcctcgt attctttatc acttaataac gtatcaagtt
     8221 cagccacatc agctagtttt acttttaata tccagttttc atatgggtct tcatttatac
     8281 attctggctc atcttctaat ttttcattaa cttccaatac ctcaccatta acaggagata
     8341 ttaaatcaga agctacttta gaagactcaa ctactccaaa atctactccc ttagttacag
     8401 tatcttctac ttctggtgtt tcaacaaaca atatttctcc caattgatct tgagcatagt
     8461 ctgttatacc tatatataca acatctccat caattacttt tacccactca tgatccttag
     8521 agtattttaa ttctggtaat aatttcattt cgatagaccc ccaatattat attattgatt
     8581 aaattactta aatcatatta atattatatc atattatttt aaattttcca tcaattacat
     8641 tacactttcc atagctaatg ctggatgacc tttttcttct aagaaagcta atactccttc
     8701 agaatcgtct gctatagttt catcacaaac catatcacag aagttttcta ttccagtcat
     8761 ttctttaact gtagcattta atttatcagc tacataatct tttaattctt ttggcatcca
     8821 aactattctt tctggtccac cttctgcata agcgaatttt ttagaagata tgaaatgtct
     8881 tccgtgtccc ataaatcctg gagtttgaac tcctccacct gtcatagaag caagttctcc
     8941 gaaagtcata ccaactggag taacacttgc aaattctctg ttaactacaa caaatccatt
     9001 tgcttctggc attataccac agatacattc gaagcatcca caagaagtca ttgggtcttc
     9061 taatatagag tatagtgtaa cagattcaac tgcaccttga gatatttggt ttacagtttc
     9121 atttactgag ttccatacac ccgttctatc atctaagcac tcgccttttt ctattggttg
     9181 acaaggacct gttgggtcta attctttagt agccttagca tctaaccagc taactgctcc
     9241 acaaagacca agtctttcag gagtaacaac acaaacgtga gctggtgcaa atgattgaca
     9301 taagttacaa gaatagaaag tatcaacact ttcatcaact aatgaagcca atctttcatc
     9361 tctagcattg tacttagcta ttgcttcacc ttttaattca gaaacttttt cagcatctgt
     9421 aattatagtt atttcacact tatcaacaac tgattcaaat tcatctaaca tcttagcata
     9481 taaaacttct cctatatgct caagtctaaa gcctttatca aatgcatctt tagagattct
     9541 tacccaagcc atatctcttt gtccaacgtg cattacacct tctatatagt ttaagaagta
     9601 gtggaaacgt ctttcaagaa ctggctcgaa atcttcttgc atatttttac cagctatttt
     9661 aactattaca gcaagtggta atcttaatac accatctgca tcaacttcat ctatatttgg
     9721 tcctattatt tctatcttat ggtcttcaac ttctgttgct tctttcttaa caacaagctc
     9781 ccatgcttct gttctgtttc caccaaactc agcaaacata tcgctctttc taattctctc
     9841 accttcaaat gctgccgcaa atgaaactgg gataggtatt tcagtaactt ttatttttat
     9901 tcctctagct tctaatgaag tagctactat cttatcgtaa tctttttgag ttaataggtt
     9961 cattggaact tcaagaactg tttggtcagt tataactggg aatcctaaag ctattgctcc
    10021 agcacctgca gatacaacta attcacttaa tggtccaaat gcattaacaa atgcaggaac
    10081 tctattagct gtgtactcta ataatccgtt taaatcacca ggagttaatc ctccgaatat
    10141 taatgctgct cttactgcaa cagatacaac gtgaataaca gaagttacat catatcctag
    10201 aggtataact cttagttcaa gacccatttt tactccagct tctatagctt ggtctataac
    10261 tttaccaact aagaaagtta gcaaaccttt agattgataa tcttttataa cttttgctgc
    10321 tgattcagca tctggacact caccaagaac aactgcaaca ccaggtatat ctcctgtaac
    10381 tagtggtaca ccaagtgatc tgattatagg gtcagttata tgacctgcac atggctcact
    10441 atatggagca tccatagttg aatattttaa tgcttcaatt acttctgcag ctaaagctgt
    10501 agctaagcca gcattgaaag catgttctag taaatgagtt ctatttatta aagattttac
    10561 tacttctaaa gcaccttcta agtcccctaa agtgttcatc ttttgtcctg ttgcagcata
    10621 tatacaaggt aatgaatatg ctgtatcagg gaaagcaact ttatgttctt ttccattttt
    10681 ttctatagct tcagctagca tagcttgagc cgcacctaaa gcttgttctg accctgtaaa
    10741 gattatatta tatagattca ttctatttcc ccctttaata tttacaccta ttttttatta
    10801 actataccct aaaaacccta gaaagcaaat ccaccttcat ttagctctaa gaaagagtct
    10861 gcatataaag atgccccgtt gattatgtca caagatttta cagttttcat taattctggg
    10921 tcacatggat tcattatagc tgaactaaat ccattggcca ttgccattgc taagaatgca
    10981 ctatctaaaa caggtctaac atgcttagga catccattag atacattaga taatcctcct
    11041 gtagttaaaa gtcccatctc agtcatcatt tttatagctt ctaaaacttc tacttgtttt
    11101 tcttgcatac cttttataac taagcataat gggtcgaata gtatatcagt tggctccatt
    11161 cctaacatta agcctttttc taatatttct tggcagtaag ccatacgctc atcattatct
    11221 cttgggatac cttcttttgc acataaacct ataaccattg cttcatattc agctgctata
    11281 tctattaaat caaatcttga accagcatca gcagagttta ttattggttt tgcattagtt
    11341 ctatcgtaaa ctttaagtcc agcttcgata gcttttttat tagctgtatc taatgctata
    11401 ggaacattgt tgaactctga ttgaagtaat ttgattcccc atgtcattat ttcttcgcca
    11461 tctctttcag caggtcctat attaaaatct atatagtgtg ctcctgcctc taattgttct
    11521 tttgctcttt ttaagattgg agctgggtct ctttctgcta atgcttttct tattgaaggt
    11581 gatatacagt gtattctttc acctataatc ataaattttt ccatttataa tgtccccctt
    11641 taaatttaat attaatagtt agttaatatt ttattagttt gtttggcatg ctttttcttg
    11701 gtatcctttt aagaacttag gtaattccat agattcttct ggtcctataa ctacattcca
    11761 tcctggtaag ttatcttcta tatcaccttt aagtactgca acttttcctg gaatgataag
    11821 gtctttacaa ttagttactt cttctacttt actttctttg atgaaagcag aaattgagtt
    11881 tccaccaaat ttaccagcag cccaagatgt aagaactgaa tatccacctg catctggtat
    11941 tactaaccat actggaactt ttgatctttc tatgtcccca gcaactatga agtaagtcaa
    12001 tgcaaagtca acagtaacta atactggaga attttcatct gggttgttga ttggataaat
    12061 ctttggttcg actctcattg gtctttgtgg gtcagtgtat atattttgtc ttagtgcaaa
    12121 taatggtaat gccttagcat aactaatatc atctattact attatagaac cgtattttat
    12181 agtgaatatt gatgataaag caacttccat catagggttt gaattagata atctatttgc
    12241 aaatactatt gaaggatatc caaatgttct atcttgctct tttaaagcta ttcttctaac
    12301 ttgtatagca ttagtgtaag tatcttttat gttttctcct gttacatcta atactaattc
    12361 tttatatcca gcagcttgaa ttaactcaac tgtttcatat aattcttcta ggctaccggc
    12421 ttttacacct agtggtaaac ttgctccttt aactacttct atcatatctt tgtagttctc
    12481 tttagttgct ccatacacca ttggtctagc atcttttaat acttcaacag cttctttaac
    12541 tacttgtgca tcatcacaag ctaaaatata agctacttct aatccactgc cttttatttt
    12601 atctattaat tttaaatacg catctttatc tccacaatac tctaaaacag ccatttcaac
    12661 tttcatttgt tcaccaattc ttacatagtc aacttttttc atgttagcta ttttagaatc
    12721 tactgcttca tcactcatac aagtacagaa tgaaactgca tatctatttc tacttactaa
    12781 tgttttttca tgtctaaata atactgtttc tccacctagt tcatactcag atgcaccagc
    12841 accaactttt aaagctttca ttaaaggtgc agtagcctct gataattttg ctatagcgtc
    12901 atcagacata tgtgggcatt tacctacttc tacagctcct gaagcaacct tcatagagaa
    12961 agccatacaa gttgggaatc cacaatcctt acagtttttc tttggtgtta atttaaatat
    13021 atctaaagct tttaatgcca ttattttttc cccctaacct taacaattta attaagctaa
    13081 tgcgttaacc aattctttta tagtttctat tgattttgga tgacgaagta taactgcgtt
    13141 tgaaccacct actaaaacac ttgctgcagt tgaaacttcc attgctatag ttctttcttc
    13201 tggacaaccc caatctggct catcttctat tggagctata gcttctttaa catgacctac
    13261 ttcaaaagct actggtgtta tgataggcat ttgtaatgtc ttatcatttt gaccaaatgc
    13321 tgcaagtcta attctatcca ttgtagatgc aacatactca tatccataac caactgctga
    13381 acatccaaca ttcataacta tattttcacc tttaacacct aattgagtta acaatacgtt
    13441 taattgttta gctaggttta tatcaacaga agattcagcc cctactttat gtgaataagc
    13501 cataccagct gatgctccta cgcccttata gttatcttct actgctgaca taaataagca
    13561 gttatgtcca tccaatgttt gagctaattt ttcaaacaat ttaccatctt tttcatgatt
    13621 tcctgaacca gctacaacta gaggtaattt tattgcttca attactgcct tagctacatc
    13681 agcacattca tcaactgact tatctaatcc atttgggtca gaaccatcaa attttaaaca
    13741 aataaaatct gcttgtgtgt tagcttcaac atatttagcc cattctactg gacatttagc
    13801 tacatctttg tataactcct tatatgaatc agtccagctt tcaggataaa cgtctgatat
    13861 ttgtatacct atttttggag gattacctac ttctccatca aaactataaa aaggtaatac
    13921 attttcccca cctaatttaa ttgctttttc ccctattcct acttcaactt ctgatatttt
    13981 tccagaatat ttttgagtag acattttaaa tgtcatattt ttttccccct taaacctatt
    14041 ttaattctaa tttagcaaaa atttctttca tagccacttt agattttgaa tcttcaggta
    14101 agttaactaa aggaatacct gtagaatcat attcatatat caattcatcc ataggtacga
    14161 caccgattaa ctctaggtta tgcttttcaa tttcttcttt gacaccatca ttcataaccc
    14221 catttggaac tttgtttaca attagaagta ttcttccaac acttaacttt agttcctcag
    14281 ctaaatctct tattctagca acagcttgta tgctacgtct tgagcaatca ctaaccaata
    14341 aaagtgtatc aacatgtcta gtgacttttc tacttagatg ttccatacca gcttcgttgt
    14401 ccatgactaa gtatttgtaa tgaccagata ttttgtttac ttgttctcta agtattccat
    14461 ttacaaaaca gtaacatcct tcaccttctg acctacccat tactaataag tcatatcctt
    14521 cgccttcttc aattatagaa tttaatctaa attgaagata ttgtgccttt gtcataccac
    14581 cagggaatgc attgcctaac ttttctcttt gatttacttc ttctcttatt tctcctattg
    14641 ttgcctcaac ttcgataccc aatacttcat ttatgttagc attggcatca gcatctacaa
    14701 ctaatactgg tccctttttg tccttaacca aataatcaat caaaagccct gtgagacttg
    14761 ttttaccagt tccacctttt cctgcaacag ctatattgta tcccatttta agacttcccc
    14821 cttaaatcta gccacagagc cttatgtact ctgtctgtct attataattt aggtacagaa
    14881 tgaacacatt ccccatgtac atcatgaagc gcttccatta gtccttcaga taaacttgga
    14941 tgtggatgta ttgcatcccc tacttgttct actgttaatc ctagatgaac agcaagtgac
    15001 aactctgtca ataaatctgt agcatgaggt cctactactg ctgcacctat tatcttatca
    15061 gtttctttat cagctataac ttttacaaat ccttggaaat gtcctatagc ctgtgctttt
    15121 ccaagacctc taaaatcaaa ttgacctaca ttgtattcta caccttcagc ttcaagttgc
    15181 ttttcagttt taccaacacc agcaacttct ggttcagtat aaacacatct tggaattgct
    15241 ctataatcta ctactttagt cttacctaaa gcattttcaa ctgctacgat accttcttta
    15301 gaagcaacat gtgctaggaa aggagtatct attatatcac ctattgcgta tataccttct
    15361 acattagttt caagatgttc attaactact acttttcctc tttccatttc aattcctata
    15421 tcttctactc cagaattatc aaggttaggt cttctaccaa cacatactaa tgcatattgt
    15481 gcttctataa cctttccatt agaaagagtt gcaacagcct taccatcaac tacttcacaa
    15541 gtttgtactc caatacctgt gataacctta attttatctt tcttaaattg tctaagtagt
    15601 tgtttagcta catctttatc ttcatttaat agtatttggt caaccatctc aactatagtt
    15661 acttctgttc ctaaggctct aaagaactgc cctatctcac atcctataac tccaccacct
    15721 actattaaca tagattcagg tatctcttca agtccaagaa cttcatcact agttattaca
    15781 acttttccat catatgggaa cattcttggt acaactggaa cagaaccatt tgctagtatt
    15841 atcttatctg cttttatagt ttctactgtt ccatcatctt tagttacttc tatagtgttt
    15901 ttatcaatta actttccaaa accattaact aagtttactc ctcttttttc aaataagaac
    15961 tctataccac ttattaattg gtttacaact ttgttttttc tttccattat ggcagtgaaa
    16021 tttggcttaa ctgtaccatc tatttctata ccaaaatcct ttgcttcttt tacagtattc
    16081 aatactcctg aagaagcaag aagtgcttta gttggtatac atcctgcgtt taagcaagtt
    16141 cctccaactc ttcttttttc aataactgtt acatctgcac caagcataga agcttttata
    16201 gctgctacat atcctcctgg tccaccacct actactacta tcttcatcct taattccccc
    16261 aaacctaata taatctacaa tcttgcaata ctataaacct gcggcatcca gtatagcagg
    16321 tatattctct tctgccccaa ttgccattat atgaacacca tcacataaat cttcttcttt
    16381 taattgtttg ataagttctc ctgccatttt tattccttca cttacccagt tttcttttcc
    16441 agctactctc aatctttcga tttgttcatc tggaacaaat attcctggta catttgcagt
    16501 cataaattta gccatccctg gtgattttaa aggtactatt cctgctaata ccttgcaatc
    16561 catatccctt gttaattctc taaactttct cattgtattt atgtcataaa gtgcttgtgt
    16621 ttggaaaaat ttagcccctg cttttatttt ctttttcatt tttaataatt gaacttctat
    16681 tggagaatat tctggagtta ctgatgctcc tagataaaaa tctggagaac cttttaattt
    16741 attaccagcc atatccgtac ctgccattaa agtctcagca gtttgaagta tccccacaga
    16801 atctaagtca aacactccct tagcttgtgg atggtctcct acgctagtat ggtctcctgt
    16861 tagtgccaac agattgttta tcccaaatac acctgctgac aacatttctc cttgtatagc
    16921 tatcctattt ctatctctcc ctgttatttg aataactggc tctaatccag catcttttaa
    16981 taatttacat gtagcaagag aagttgctct cataactgcc gattgaaaat ctgttacatt
    17041 tgctgcatga actcttccaa ctaacggctt tgcacattct attaaatgtg aaaggtcagt
    17101 cccttttgga ggagccattt cagtagtaac tgcaaatttt ccactttcta atgtttctct
    17161 taataagctc atctctaatc ccccttttaa ccttctaaaa aaaactttta tgaatttgct
    17221 gctgcttctt tctttttctt tgcacttaaa cttcttggat tattttgctt agaatagtct
    17281 tttggtggtc ttatttctac taagttatct aattgtccta tagcttctaa tctttcatat
    17341 atctttatcc aagcacagtc attctctgcg tttacttcac acttaccatt tttagctcca
    17401 ccacaagccc cattcataag acctttagca cacatagtta ctggacatat cccacctgtc
    17461 caaccaagtt cacagtctcc acaagcctta caggcttctt catattctcc aactctttgt
    17521 acttccccta taaacaaagt attgttggct ggataaactg gcttatcttt tagattctta
    17581 actattgtct gtgtaccatc tccacatgct aaagataaaa ctgcatcagc ttccttagtc
    17641 tcttctttta gagcctttaa atccttttta gactttaaaa ggttacaagc agggtcaagc
    17701 attacatatc ctaagacttt tttgccttca ccttcaagag tttccttcat ttttaaaact
    17761 tcttcttctc caccactttt acaagttgct gcacattgat tacaaccaac taaaaccagc
    17821 ttgtcaaaat ccttcaagta ccctagcact tcttctaatg gtttattttc agaaattatc
    17881 atatctactt cccccctaaa ccttccacat atatatctat ttattgtttt gtaatttaca
    17941 agccttaact acatgttttg caagtataga tgttgtaact gaaccaaccc ccgctggaac
    18001 tggagttatc attgatactt tgtttaaaac tgcatttgta tcaacatctc cacataaatt
    18061 tccttcttca tcaacattta taccaacgtc tataactact gccccatcct taacaaagct
    18121 ttcatcaacc atcttagctc taccaatagc tgctattaat acatcagcct ctgcagctac
    18181 acttgataga tttttagttt ttgaatgaca tattgtaaca gttgcatgtt cgcttaataa
    18241 aagcattgat actggcttcc caactaccat tgaccttcca agaactgtta ccttacttcc
    18301 ttttaaatca acattataat gttttaatat ctccactact gctgtaggag tacatggtgg
    18361 aaatccactt ttatctccct ccattacctt agcagagttt attgggctaa aacagtctac
    18421 atctttttct ggtgcaataa catattttat aacttcttca tttaagtgct ttggaagagg
    18481 tctaaaacat aatataccat taacattttt gtcatcatta actctcttta atacatcaat
    18541 atattcatct tgtgttatat cctcagctaa ctctaacact tctgttgtta taccaatgtt
    18601 ttgacatctc tttaatgctc ctctttcata agataaatca tcacttcttg ccccaactct
    18661 aactattgtt agtttcggat ttataccttc tttaactagt aaatcgactt cttttattaa
    18721 ttcctcacta attttatcag ctacaggttt tccttttata atttgtcctt tagttgacat
    18781 tccttccata cattcacaac tcccctacaa gcaaacttaa attaatttat tctatatacc
    18841 atatattaat aacatagtat ctactttcgt catttaaaaa ttattttcca agagcttttt
    18901 caactttggt ataaacttca tcacaaattt ttacaccatc ttcaactaac ttatctactt
    18961 ctgtttttac tttatcaaca tattccttgt cttgtataga atttatattt ataactacat
    19021 ttaattgtcc tccaagtata gcagctctta aacactgaac tcctacacca acatcactta
    19081 tagcaagtct tgagccttta tcaactaaat cttcatgtaa tttaatagct tcatagcaaa
    19141 ctcttactat gtttaatggt acttcacaag caacttttaa tgctttttcc attgtttctg
    19201 cttttattct tttttcttct tcggtttctt taggaagccc gtaagctttt gataatggta
    19261 agaaacattc tgcatcatca tctatcattt ttaataaatc tttttctaac tttcctgcct
    19321 tacttagtat ctcttttaca ctttcttcat actcagcata cttcttcttc cctattgtaa
    19381 gattacatac catactgcct agagccatac ctattgcacc tactagtgca gcagctcctc
    19441 cgccacctgg tactgcttct ttagaagata gtacttctac aaaatcaaca caagttttat
    19501 ctgctatttt catgataatc ctccttaaaa ggatgtagga aaatatctca aattatttta
    19561 gagatatttc ctactcttta tttataaatt aatttcctta gaataatcca gctattactc
    19621 cattttcatc aacatctatt ttttctgctg ctggaacttt tggtaatcct ggcatcttca
    19681 ttatttctcc agtaagtgca actataaatc cagctcctgc tgagattgtt gcttgtctta
    19741 cagttattct aaatcctgtt ggtctaccta atttagtttg gtcatcagtt aaagaatatt
    19801 gagtcttagc catacatact ggaactttag taaatccaag tctttctagg ttagctattt
    19861 ccttatcagc ttctggagta taatctacac catctgctcc ataaatctta gtagctatag
    19921 catttatctt gtcttttata gataaatcat cttcatagca gaatttaaag ttattttctt
    19981 cttcatccaa taatcttaaa acttctttag ctactgcaat tccaccttct ccaccttttg
    20041 cccatacttc agaaagagca acatttacac ctaatgcctt acacttgtct tcaacaagtt
    20101 taagttcagc ttcagtgtca agtgggaatc tgttaattgc aacaactgct ggtaatccat
    20161 atacttgagt tatgttttca acatgtttta ataagtttgg aagtcctttt tcaagagctt
    20221 ctaggttttc ttcatttaag tctgctttag caactccacc attgtatttt aatgctctta
    20281 cagtagcaac tattataaca gcatctggtt ttaagttagc cattctacat tttatatcta
    20341 agaatttttc tgctccaagg tcagcaccga aaccagcctc agttattaca taatctgcaa
    20401 agtgcatagc cattcttgta gctataacag agttacatcc atgagctata tttgcgaatg
    20461 gtccaccatg tacaaatgat ggtgttcctt ctagagtttg aactaggttt ggttttaaag
    20521 catcttttaa taatgcagcc atagcaccgt tagcttttaa ttgtctagct gttacaggtt
    20581 caccttcgta actgtatcca actattatat ttcctaatct ttcttttaaa tcagatatat
    20641 cacttgccaa acagaacgct gccattattt cagaagcaac agttatatcg aatccatctt
    20701 gtctaactgc tccatcacct tttttaccca taccatcaac gatatttcta agttgtctat
    20761 cgttcatgtc tacacatctt ctccaagtta ttttcttagg gtctattcta agtgcatttc
    20821 cttggtgaat atggttatca agcatagcag ctaaaagatt gttagcagct cctatagcat
    20881 gaaaatctcc tgtgaagtgt aagtttatat cttccatagg aacaacttgt gcatatcctc
    20941 cacctgctgc tccaccttta acaccaaata ctggtcctag agatggttct cttaaagcaa
    21001 ctaatacatt tttatctaat ttagcaaatg catcagcaac acctatagta gtagtagttt
    21061 ttccttcacc tgctggagtt gggtttatag ctgtagttag tattaactta gcttttttac
    21121 cagtttctct ttttagtaga ttgtagtcaa cttttgcttt gtatttacca taaagttcta
    21181 catcatcttc acctagtcct aatttttttg caacctctct aatatcttga ggcttagctt
    21241 cttgagctat ttcaatgtca gatttaaatc ccataactga atctcctccc acaacctaaa
    21301 tagatttaaa atataatttt tgtgaaaatt gtattaatat gtaaaaatta tattttataa
    21361 gataattata catgtaaaat tacttaaaaa ctagctacta agctattaaa atattcaaac
    21421 ttaatagcta gttatttatc taattactta cttattacct atctattgcc tatgtctaac
    21481 ctttaaccaa cttaaaatcc caaatcaaaa attctattta ttttatttat ctttaagtga
    21541 cattaattta tcttttattg ccttaatttg ttctttcgtt gtttcactag aatcatatgg
    21601 agattgatta gctctatcag aatcaataac atcttggtta taataaataa atcctaaaga
    21661 ttctccatct tctaagtttt ggattataaa ttcttcatct tccttatttc taattttatt
    21721 tcccactaca aatactttat ttacacctat atcatgtcct agtttcttaa cttttctata
    21781 agtctgtaga cttctctctc ctggttctac aactactata aaagcatcta caccttgggc
    21841 tgttcctctt cctaagtgtt caattcccgc ttccatatcc ataaccacta catctttatt
    21901 ttgtagtatt agatgtgagc agagcctctt caaaagaaca tgctctggac atacacatcc
    21961 tgtaccacca gaatctactg ttcccaatgt aagtaatctt acaccattgt attctttaca
    22021 gaaattttca gggatatcgt ctactttagg attcatctta aacatttttc caaatgaacc
    22081 tacagaagca gctgttctat ctgaaactaa ttttttcatt tctgatatag gtactattga
    22141 ttcatatact tcttttggaa aacctagtgc taaagcta
//
LOCUS       DAFNNB010000053        21942 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0053, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000053 DAFNNB010000000
VERSION     DAFNNB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21942
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0053"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            366..677
                     /gene="rpsJ"
                     /locus_tag="L3M60_001721"
     CDS             366..677
                     /gene="rpsJ"
                     /locus_tag="L3M60_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBN6226396.1"
                     /translation="MAKNEKIRIRLKSYDHKLLDFSAGKIVETAKKAGSQVSGPVPLP
                     TEKQVVTILRAVHKYKDSREQFEIRTHKRLIDIANPTPKTVDSLMRLDLPAGVDIEIK
                     L"
     gene            769..1398
                     /gene="rplC"
                     /locus_tag="L3M60_001722"
     CDS             769..1398
                     /gene="rplC"
                     /locus_tag="L3M60_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBN6226397.1"
                     /translation="MKGILGKKVGMTQIFTDKGVVIPVTAVEAGPMVVTQIKTVDKDG
                     YNAIQIGFEDAKEKALNKPKKGHLAAANVLKKHLKEFRVDSVEGYTVGQEIKADVFEA
                     GAKIDVTGISKGKGFQGPIKRHGQSRGPETHGSRYHRRPGSMGACSYPGRVFKNKKLA
                     GHMGSVKVTVQNLEVVKVDADKNLILVKGAIPGAKGSVVTIKEAIKVSK"
     gene            1428..2051
                     /gene="rplD"
                     /locus_tag="L3M60_001723"
     CDS             1428..2051
                     /gene="rplD"
                     /locus_tag="L3M60_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBN6226398.1"
                     /translation="MPKLNVLNVSGQNVGEIELSDSIFGVEVNGHVLYEVVKNQLANK
                     RQGTQSAKTRAEVRGGGRKPWKQKGTGRARQGSTRSVQWVGGGVAFAPKPRSYKYTLP
                     KKVRRLAMKSALSSKVQNSEVIVLDALNMDAPKTKEFAQILNNINAAKKALVVIADKN
                     DNIIKSARNIEGVQTALVNTMNVYDILKYDSFIITTDAVKKVEEVYA"
     gene            2051..2341
                     /gene="rplW"
                     /locus_tag="L3M60_001724"
     CDS             2051..2341
                     /gene="rplW"
                     /locus_tag="L3M60_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBN6226399.1"
                     /translation="MTNPHDVIIRPVVTEHSMAEMGEKKYTFVVAKDANKTEIKKAVE
                     KVFGVSVDKVNTLNYDGKVKRMGRTQGRTASFKKAVVKLTADSKEIEFFQGM"
     gene            2371..3201
                     /gene="rplB"
                     /locus_tag="L3M60_001725"
     CDS             2371..3201
                     /gene="rplB"
                     /locus_tag="L3M60_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBN6226400.1"
                     /translation="MAIKKFRPTSPALRQMTVLVSDEITCNQPEKSLLVNLKKNAGRN
                     VHGRITVRHRGGGQKRKYRIIDFKRDKDGIPAKVATIEYDPNRTANIALLNYADGEKR
                     YILAPVGINVGDTILSGLGADIKPGNCLALKDMPVGTIIHNIELKPGKGAQLVRSAGV
                     SAQLMAKEGKNALLRLPSGEMRLVSINCKATIGQVGNIEHGNVVIGKAGRKRHMGIRP
                     TVRGSVMNPNDHPHGGGEGRSPIGRPSPVTPWGKPALGYKTRKKNKASNKLIVSRRTK
                     "
     gene            3236..3517
                     /gene="rpsS"
                     /locus_tag="L3M60_001726"
     CDS             3236..3517
                     /gene="rpsS"
                     /locus_tag="L3M60_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003454422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBN6226401.1"
                     /translation="MSRSTKKGPFVHARLLKKIEAMNASGNKEVIKTWSRSSTVFPQM
                     VENTIAVHDGRKHVPVYITEDMVGHKLGEFVPTRTFKGHKDDEKSNKRK"
     gene            3549..3884
                     /gene="rplV"
                     /locus_tag="L3M60_001727"
     CDS             3549..3884
                     /gene="rplV"
                     /locus_tag="L3M60_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBN6226402.1"
                     /translation="MEAKATAKYVRVSPRKAGQICDLVRGKNVDEALAILKFTPRGAA
                     SIIAKVVKSAKANAENNHEMDTEKLYIASIVANQGPTMKRFMPRAMGRATTIRKRTSH
                     IEVVVKEKK"
     gene            3908..4723
                     /gene="rpsC"
                     /locus_tag="L3M60_001728"
     CDS             3908..4723
                     /gene="rpsC"
                     /locus_tag="L3M60_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBN6226403.1"
                     /translation="MGQKVNPHGLRVGVIKDWDSRWFATDKKEFGNLLLEDHNIRKFL
                     KKRLYSAGVAKIEIERSANKIKMDLHVAKPGVVIGRAGAGIEALKAELEKMTKKTIIV
                     NIVEVRSTDKNAQLVAENIALAIERRVAFRRAMKQAIQRAMKSGAKGIKVSASGRLGG
                     AEMARTEGYSEGNVPLQTLRADIDYGFAEADTTYGKIGIKVWICNGEVLPTRDGVNPR
                     EESRKSDRRDNKRDNRRNDRRGNDRRGNDNRGNYRGQRPQGGSRPQRTENKGN"
     gene            4759..5190
                     /gene="rplP"
                     /locus_tag="L3M60_001729"
     CDS             4759..5190
                     /gene="rplP"
                     /locus_tag="L3M60_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBN6226404.1"
                     /translation="MLMPKRVKRRRVHRGSMAGQAHKGNKVTYGEFGLVALEASWITS
                     NQIEAARIAMTRYIKRGGKVWIKIFPHKPVTRKPAETRMGAGKGSPEYWVAVVKPGRV
                     MFELAGVSEDKAREAMRLAAHKLPIKCKFVKKEDLEVKGGE"
     gene            5192..5395
                     /gene="rpmC"
                     /locus_tag="L3M60_001730"
     CDS             5192..5395
                     /gene="rpmC"
                     /locus_tag="L3M60_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBN6226405.1"
                     /translation="MKAKELRDLTSEELMNKLNDFKSELFSLRFQLATGQLENTARIK
                     FVKKDIAKVKTVLAERKLYETRA"
     gene            5419..5673
                     /gene="rpsQ"
                     /locus_tag="L3M60_001731"
     CDS             5419..5673
                     /gene="rpsQ"
                     /locus_tag="L3M60_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBN6226406.1"
                     /translation="MERGRRKVRIGRVVSDKMDKTIVVAVEEFVRHPLYNKRVKRTKK
                     FKAHDEKNICNIGDRVKIMETRPLSKDKRFRLVEVVEKVK"
     gene            5699..6067
                     /gene="rplN"
                     /locus_tag="L3M60_001732"
     CDS             5699..6067
                     /gene="rplN"
                     /locus_tag="L3M60_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007885948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBN6226407.1"
                     /translation="MIQQESRLRVADNSGAKELLCIRVLGGSKRRYGNIGDVIVATVK
                     SATPGGVVKKGKVVKAVIVRSKQGVRRNDGSYISFDENAAVIIKDDKTPVGTRIFGPV
                     ARELRDNEFMKIVSLAPEVL"
     gene            6091..6396
                     /gene="rplX"
                     /locus_tag="L3M60_001733"
     CDS             6091..6396
                     /gene="rplX"
                     /locus_tag="L3M60_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBN6226408.1"
                     /translation="MRVKKGDTVVVIAGKDKGKKGSVLKVYPKTSKVLVEGVNVITKH
                     QKPSAMNQQGGIINKEAPIHISNVMPFDPETGKGVRVRYEVKDGNKVRVSAKSGKEL"
     gene            6426..6968
                     /gene="rplE"
                     /locus_tag="L3M60_001734"
     CDS             6426..6968
                     /gene="rplE"
                     /locus_tag="L3M60_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBN6226409.1"
                     /translation="MASRLQEKYMKEVAPALMEKFGYKNVMEIPKLNKIVINMGIGDA
                     RENPKGLEKGVEELEIISGQKPVITKARKSVANFKLREGMPIGTKVTLRADKMFYFMD
                     KLVSVSLPRVRDFRGVNPNAFDGRGNYALGVKEQLIFPEIEYDKIDKVRGMDIIFVTT
                     AKTDEEARELLKLLGMPFSK"
     gene            6986..7171
                     /locus_tag="L3M60_001735"
     CDS             6986..7171
                     /locus_tag="L3M60_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HBN6226410.1"
                     /translation="MARKAMVVKQQRKQKYATREYTRCTICGRPHSVLKKFGICRICF
                     RELAYKGQIPGVRKASW"
     gene            7204..7602
                     /gene="rpsH"
                     /locus_tag="L3M60_001736"
     CDS             7204..7602
                     /gene="rpsH"
                     /locus_tag="L3M60_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBN6226411.1"
                     /translation="MTMTDPIADMLTRIRNANVVKHETVDVPASNMKKELARILLEEG
                     FIRGYDVIEDGKQGIIRIQLKYGQEGERVITGLKKISKPGMRVYAANHEIPKVLNGLG
                     ISVISTSKGILTDKQARKENVGGEVICYVW"
     gene            7633..8175
                     /gene="rplF"
                     /locus_tag="L3M60_001737"
     CDS             7633..8175
                     /gene="rplF"
                     /locus_tag="L3M60_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBN6226412.1"
                     /translation="MSRIGVKPIIVPAGVEVTIAEGNLVTVKGPKGTLTKQLSAELNI
                     KKEENTIMVERPTDNKKHRSLHGLTRTLLDNMVVGVNTGFEKKLELKGVGYRAQKQGK
                     KLVMNLGFSHPVEMEDPEGITVEAPNQTELIVKGIDKQLVGNYAAKIRAWRKPEPYKG
                     KGIKYVDEVIRRKEGKTGKK"
     gene            8211..8579
                     /gene="rplR"
                     /locus_tag="L3M60_001738"
     CDS             8211..8579
                     /gene="rplR"
                     /locus_tag="L3M60_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBN6226413.1"
                     /translation="MLKKADKNANRLQRHKRVRRKISGTSQRPRLCVFRSANNIYAQI
                     IDDTKRVTLVAASSLEAEVKSAVNHTGNKEAAKKVGELVAKKAVEKGITEVVFDRGGY
                     LYHGRIQELAEGAREAGLKF"
     gene            8600..9109
                     /gene="rpsE"
                     /locus_tag="L3M60_001739"
     CDS             8600..9109
                     /gene="rpsE"
                     /locus_tag="L3M60_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBN6226414.1"
                     /translation="MLRRKPIDAGQLDLQEKVVEVRRVTKVVKGGRNFRFAALVVVGD
                     ENGHVGIGAGKAMEVPDAIKKAVEDAKKNLIVVPIVGTTIPHEVRGHFGAGNILIMPA
                     VEGTGVIAGGPARAVLELAGLKDVRAKSLGSNNPRNMVNATIEGLNSLKTVEDIAKLR
                     GKKVEELLG"
     gene            9124..9309
                     /gene="rpmD"
                     /locus_tag="L3M60_001740"
     CDS             9124..9309
                     /gene="rpmD"
                     /locus_tag="L3M60_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBN6226415.1"
                     /translation="MAKLQIKLVRSVIGTTPNQKKNVEALGLRKREQVVVKEDNAQTR
                     GMINKVSHLLEVTEIAE"
     gene            9342..9785
                     /gene="rplO"
                     /locus_tag="L3M60_001741"
     CDS             9342..9785
                     /gene="rplO"
                     /locus_tag="L3M60_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBN6226416.1"
                     /translation="MKLHELKPAEGAVRAKRRLGRGTATGQGKTAGRGQKGQWSRSGG
                     GVRVGFEGGQMPLARRLPKRGFNNIFKKVYTEVNVEVLNRFENGTEITAELLKSTKTI
                     SKIGKDGIKILGEGNLEKALTVKAAKFTASAQEKIEKAGGKAELV"
     gene            9829..11097
                     /gene="secY"
                     /locus_tag="L3M60_001742"
     CDS             9829..11097
                     /gene="secY"
                     /locus_tag="L3M60_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBN6226417.1"
                     /translation="MLSKLKQAWKIKAVRKKVMYTLMMIVIFRIGTTIPVPGIDTSII
                     QKMVGGNSLLSLYNMFTGGAFSNFSLFALGISPYITASIIIQLLTVGFESLAELQKSG
                     EEGKKKINKYTKYTALALAVVQALGITLGIVRSALISNSVFFITTVVITLVSASMLVM
                     WIGDKITEKGIGNGSSVIIFAGIISRIPTDVIKISQQVKSGQVAPWVIVILAVVILLT
                     VTGVTFIQEATRKIPVQYAKRVVGRKMYGGQSSHIPMKVNQSGVMPIIFASSLLAFPQ
                     TIAMFMGPNAQAFVQKYLSMATEQGFWTYRSIEILLIIFFSYFYTTVSFNTEDISKNM
                     KNNGGFIPGIRPGEPTMDYLNRILTRLTLAGATFLAIIAMVPALTTHYMKVNMSLAGT
                     SLLIVVGVALELKRQLESNLVMRSYQGFLK"
     gene            11114..11764
                     /locus_tag="L3M60_001743"
     CDS             11114..11764
                     /locus_tag="L3M60_001743"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBN6226418.1"
                     /translation="MRIILLGPPGAGKGTQAAGIVEKYNIPHISTGDIFRKNIKEGTE
                     LGKKAKEYMDQGLLVPDELTVGLVTDRISQEDCKNGFMLDGFPRNVAQGEHLDIFLKN
                     AGISLDKVVNIEVDKSILVSRAVGRRICKSCGATYHVEFNPPKVEGVCDVCQGELYQR
                     ADDNEETVSKRIQVYLDETKPLVNYYSKQGIIADIKGDQAIDKVFEDIVTALGSGK"
     gene            11765..12511
                     /gene="map"
                     /locus_tag="L3M60_001744"
     CDS             11765..12511
                     /gene="map"
                     /locus_tag="L3M60_001744"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBN6226419.1"
                     /translation="MIILKSKKEIELLREAGKIVADTHEVLRKAISPGISTLELDKIA
                     EENIRKYNAEPSFKGYGGFPGSICASINREVVHGIPGETILQEGDIVSLDIGAYYKGY
                     HGDSAKTHGVGMISEEDRKLIEVTRESFYEGIKFAKLGYRLSDISHAVQTHVEKHGFS
                     VVRDLVGHGVGANLHEDPQVPNYGLPGKGPRLREGMVIAIEPMVNAGRYHVKTLSDGW
                     TTVTIDGKKSAHYEHTIAITEHEPLILTKL"
     gene            12533..12802
                     /locus_tag="L3M60_001745"
     CDS             12533..12802
                     /locus_tag="L3M60_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KOW domain-containing protein"
                     /protein_id="HBN6226420.1"
                     /translation="MLSDNLSIGQVVKVSLGRDKGNLFFVVKIINNEYVLIADGKKRK
                     LDKPKLKKVKHLKKYNFINDEVRKRVVSGQEITDSFLRAELTKLN"
     gene            12840..13058
                     /gene="infA"
                     /locus_tag="L3M60_001746"
     CDS             12840..13058
                     /gene="infA"
                     /locus_tag="L3M60_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBN6226421.1"
                     /translation="MAKKDVIELEGTVSEALPNAMFKVKLENGHEILCHISGKLRMNF
                     IRILEGDKVNVELSPYDLTRGRITWRKK"
     gene            13094..13207
                     /gene="rpmJ"
                     /locus_tag="L3M60_001747"
     CDS             13094..13207
                     /gene="rpmJ"
                     /locus_tag="L3M60_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBN6226422.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQKQG"
     gene            13360..13731
                     /gene="rpsM"
                     /locus_tag="L3M60_001748"
     CDS             13360..13731
                     /gene="rpsM"
                     /locus_tag="L3M60_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBN6226423.1"
                     /translation="MARIAGVDLPREKRAEIGLTYIYGIGKATANEILAKAEINPDTR
                     IKDLSEDQVNELRKVIDDDFLVEGDLRREIALNIKRLRDIKCYRGIRHAKGLPLRGQR
                     TKTNARTRKGPRKTVSRKKKK"
     gene            13767..14165
                     /gene="rpsK"
                     /locus_tag="L3M60_001749"
     CDS             13767..14165
                     /gene="rpsK"
                     /locus_tag="L3M60_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBN6226424.1"
                     /translation="MAKPKKKVTRIRRRERKNIERGHAHIQSTFNNTIITLTDVHGNA
                     ISWASSGQLGFKGSRKSTPFASQMAAETAAKAAMEHGLKSVEVFVKGPGSGREAAIRA
                     LQATGLEVTMIKDVTPIPHNGCRPPKRRRV"
     gene            14197..14820
                     /gene="rpsD"
                     /locus_tag="L3M60_001750"
     CDS             14197..14820
                     /gene="rpsD"
                     /locus_tag="L3M60_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBN6226425.1"
                     /translation="MARYTGASCRQCRREGMKLFLKGDRCYTDKCAIVKRNYAPGQHG
                     QGRKKVSNYGLQLREKQKVKRIYGVLETQFRNLYERAENMPGKAGENLLSLLERRLDN
                     VVYRMGLASSRKEARQLVTHGHFTLNGNKVDIPSLIVKVGDVIEVKEKSRSSAKFKNL
                     VEVNSRIAPKWLEANVEGMTAKVVGVPTREDIDLEIAEHLIIELYSK"
     gene            14898..15845
                     /locus_tag="L3M60_001751"
     CDS             14898..15845
                     /locus_tag="L3M60_001751"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBN6226426.1"
                     /translation="MIEIEKPKVDIVELSEDYRYGKFVIEPLERGYGITIGNALRRIL
                     LSSLPGVAVNAIKIDGVLHEFSTIPGVKEDVTEIILTLKELSATIDGEGSRTLKIEAQ
                     GPCSITGADIICSPDVEILSKDLAIATLDDNAKLNMEIFVDKGRGYVSAEENKTENVP
                     IGVLPVDSIYTPVEKVSYHVENTRVGQKTDYDKLVLEVWTNGSINPQEGISLAAKVLV
                     EHLNLFIDLTEHVSSVEIMVEKEEDQKEKVLEMTIEELDLSVRSYNCLKRAGINTVEE
                     LANKSEDDMMKVRNLGKKSLEEVIQKLEELGLGLKPSEE"
     gene            15866..16207
                     /gene="rplQ"
                     /locus_tag="L3M60_001752"
     CDS             15866..16207
                     /gene="rplQ"
                     /locus_tag="L3M60_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003357477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBN6226427.1"
                     /translation="MAKYRKLGRETAHRNLMLRNLVTCLLRSGRIETTVTRAKETRRM
                     AEKMITLAKRGDLHARRQVLAYVMDETVVNNLFTDLAPKYAERNGGYTRIIKIGPRKG
                     DAAEMAFIELV"
     gene            16327..17160
                     /locus_tag="L3M60_001753"
     CDS             16327..17160
                     /locus_tag="L3M60_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBN6226428.1"
                     /translation="MDNIVKVNNISFEYITDEAKLKAIDNLSLDVKKGEFVAIIGHNG
                     SGKSTLSKNLNAILMPTEGNILIDGMNTKEEERLWDIRQTAGMVFQNPDNQIVATIVE
                     EDVAFGPENLGIEPKEIRRIVEESLKSVGMYDLRDRQPHLLSGGQKQRVAIAGIIAMR
                     PKCIIFDEATAMLDPSGRKEVMKTIKRLNKEENITVIHITHFMEEAVEADRVVVMEKG
                     KKILEGTPREVFSKIKMLKEIGLDVPCMTELSSLLIEEGINISGDILTVDEMVMELCR
                     L"
     gene            17148..18014
                     /locus_tag="L3M60_001754"
     CDS             17148..18014
                     /locus_tag="L3M60_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HBN6226429.1"
                     /translation="MSIIVKNLTHIYNEGMPFASKALDDISFEIKDRDFVGLIGHTGS
                     GKSTLIQHLNGLLKPSSGEIFINDFNITDKNLNLTEIRKRVGVVFQYPEYQLFEETID
                     KDIAFGPSNLGLEESEIHNRVKASMEAVGLDYEEFKDKSPFELSGGQKRRVAIAGVIA
                     MNPEVLILDEPTAGLDPGGRDEIFNLIKSLHEKKNMTIILSSHSMDDMAKLAKTLIVM
                     NHGSVEFIGTPREVFKSNASKLKDIGLDIPQVLELALKLREKGFDISEDILTLEEAKQ
                     EILKVVRGRGLC"
     gene            18008..18811
                     /locus_tag="L3M60_001755"
     CDS             18008..18811
                     /locus_tag="L3M60_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBN6226430.1"
                     /translation="MLKDITIGQYYPTSSAIHKLDPRIKLVATIVFMISIFVVNKFWP
                     YIVVLLCLLAMIKLANIPIKYIVKGVKPLKWIILFTFFINIFFLPGDEIWSFGFLTIT
                     SQGLRQAIFMAIRLIFLVVGTSLLTLTTSPIELTDGIERLLNPFKKIGLPVHELAMMM
                     TIALRFIPTLLDETDKIMKAQMSRGADFESKNLINRAKNLVPLLVPLFVSAFRRADEL
                     AMAMEARCYRGGHNRTKMRESVISRRDYMACVFQVVYLGAIIATRFIAI"
     gene            18844..19575
                     /gene="truA"
                     /locus_tag="L3M60_001756"
     CDS             18844..19575
                     /gene="truA"
                     /locus_tag="L3M60_001756"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBN6226431.1"
                     /translation="MRNIKITIQYNGKNYCGWQKQLDSLGIQGTIERAIYDITKEKVS
                     LIGSGRTDSGVHAIGQIANFKTNSSISIESIPMALNAKLPKDISVIDACEVNDDFHSR
                     YSAKGKTYKYLVYNSKFRNPILSEISYQVKYELDFDKMCSEAKLLLGTHDFKGFMSSG
                     SSVKDTVRTIYDIDIIRKDDLITFEISGNGFLYNMVRIIVGTLVDIGRGRINEPFLDI
                     IQSKTRSRCGHTAPAQGLFLKKVHY"
     gene            19695..20126
                     /gene="rplM"
                     /locus_tag="L3M60_001757"
     CDS             19695..20126
                     /gene="rplM"
                     /locus_tag="L3M60_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBN6226432.1"
                     /translation="MKSYIAKPADVQRKWYLVDAEGKTLGRLATEIATVLRGKHKPTF
                     TPHVDGGDFVVVVNAEKIVLSGKKLDQKYYRYHTGYVGGLKEISYRDMMDKKPEEVIS
                     HAVSGMLPKNKLRSRMMTRLRVFAGAEHTHAAQNPEVLNFK"
     gene            20155..20547
                     /gene="rpsI"
                     /locus_tag="L3M60_001758"
     CDS             20155..20547
                     /gene="rpsI"
                     /locus_tag="L3M60_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBN6226433.1"
                     /translation="MANVQYYGTGRRKSSVARVRLVAGEGNILVNGRALENYFNYETL
                     IRDVKQPLVLTGNENKYDVIVKVEGGGFTGQAGAIRHGISRALLKADLDLRPALKKEG
                     FLTRDARMKERKKYGLKAARRAPQFSKR"
     gene            21056..21760
                     /gene="cwlD"
                     /locus_tag="L3M60_001759"
     CDS             21056..21760
                     /gene="cwlD"
                     /locus_tag="L3M60_001759"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase CwlD"
                     /protein_id="HBN6226434.1"
                     /translation="MRKYIKHIIFSFAMICLVVVSIFEIKNISEDVIKYMPVTNKTII
                     LDAGHGGIDPGALNKDKSTSEKDINLAITLKLRELIESSGGLVILTREDDSSLYKEEN
                     NKTTRQKYNENLKNRKEIISNSNANMFVSIHLNAFEQSKYYGAQTFYPKDKQDSKELS
                     KCIQEELKRVVDKTNNREVKPRDDIYLLKENNIPSVLIECGFLSNEKESKLLTDETYQ
                     EKIAWAIYIGIQKYLS"
BASE COUNT         8799 a         2565 c         4504 g         6074 t
ORIGIN      
        1 ctttactagc taatggattc aatataaaaa agagggggta acacctctct tttttgtttt
       61 ttattgacaa gtagctctaa agcatatata ctaaactagt gccttgagat agattaacaa
      121 tcaatttaag gttatgatgt tgtaatagaa aataaaattg aaaaaaagat aaaaaaaact
      181 tgaaaaaatg taacagatga tataaaatat aatagtgtgc ttgagagatt aagaaaataa
      241 gaattaaatc ctagtgaaat acgaactgat atcgatttga aagttacaac acacccggag
      301 cagtgtatcg gtattggttg tacgcagctt gaaaattaaa cacgtgataa aggagggaaa
      361 acaaaatggc taagaatgaa aaaataagaa taagattaaa atcatatgat cacaaattat
      421 tagatttttc agcaggtaaa atagttgaaa ctgctaaaaa ggctggatca caagtttcag
      481 gacctgtgcc tctaccaaca gaaaaacaag ttgtaactat attaagagct gtacataagt
      541 acaaagactc tagagaacaa tttgaaataa gaactcataa aagactaatc gacatagcta
      601 atccaacacc taagacagtt gactcattaa tgagattaga cttaccagca ggtgtagata
      661 tagaaataaa attataagaa cccctaagat attatcctat agatgattat cttagaatga
      721 ttactctaaa agatgtaatc cgctgtaaga attataggag gtgctaatat gaaaggaata
      781 ttaggcaaaa aagttggtat gactcaaata ttcactgata aaggtgtagt tatacctgta
      841 acagctgttg aagcaggacc tatggtcgtt actcaaataa aaacagttga taaagatgga
      901 tacaatgcta tacaaatagg ttttgaagat gctaaagaaa aagctttaaa caaacctaaa
      961 aaaggacatt tagctgctgc taatgtttta aagaaacatt taaaagaatt tagagtagat
     1021 tctgtagaag gatacacagt aggacaagaa ataaaagctg atgtatttga agcaggtgca
     1081 aaaatagatg ttactggaat aagtaaaggt aagggattcc aaggtccaat aaaaagacat
     1141 ggacaaagta gaggtcctga aactcacggt tctagatacc acagaagacc aggttcaatg
     1201 ggagcttgtt cttacccagg tagagtattt aaaaacaaaa aattagctgg acacatggga
     1261 agcgtaaagg taacagttca aaacttagag gttgttaagg tagatgctga taagaacctt
     1321 atattagtta aaggagctat accaggagct aaaggttcag tagtaactat aaaagaagct
     1381 ataaaggttt ctaaataatg actaacctag agaaaggagg aataacaatg ccaaaattaa
     1441 atgtactaaa tgttagtgga caaaacgttg gagaaataga attatcagat tctatatttg
     1501 gcgtagaagt taatggacat gttttatatg aagttgtaaa aaatcaatta gcaaataaga
     1561 gacaaggaac tcaatctgct aaaactagag cagaagttag aggtggcgga agaaaacctt
     1621 ggaaacaaaa aggaacaggt agagcaagac aaggttctac aagatctgta caatgggtag
     1681 gtggaggagt tgcttttgca cctaaaccaa gaagttacaa atatacatta cctaagaaag
     1741 taagaagatt agctatgaag agtgctttat cttcaaaagt tcaaaatagt gaagttatag
     1801 tattagatgc attaaacatg gatgctccta agactaaaga atttgctcaa atattaaaca
     1861 atataaatgc tgctaaaaaa gctttagtag ttatagctga caaaaacgat aacataataa
     1921 aatcagctag aaatatagaa ggtgttcaaa ctgcattagt taatactatg aatgtatatg
     1981 atatattaaa atatgattca tttataataa ctacagatgc tgttaaaaaa gtggaggagg
     2041 tgtacgcata atgactaatc cacatgatgt aataataaga ccagttgtaa ctgagcacag
     2101 tatggctgaa atgggtgaaa aaaaatacac ttttgttgtg gctaaagatg cgaacaaaac
     2161 tgaaataaaa aaagcagtag aaaaagtatt cggagttagt gttgataaag taaatacttt
     2221 aaactacgat ggaaaagtta aaagaatggg tagaactcaa ggaagaacag caagctttaa
     2281 gaaagcagta gttaaattaa ctgctgatag taaagaaata gaattcttcc aaggaatgta
     2341 gtattataac aaacgaagga gggaaaacag atggctataa aaaagtttag accaacttct
     2401 cctgccttaa gacaaatgac agttttagtt tctgatgaaa taacatgcaa tcaaccagag
     2461 aaatctcttt tagttaactt aaagaaaaat gctggtagaa acgtacatgg tagaataact
     2521 gttcgtcata gaggtggagg acaaaaaaga aaatatagaa taatagattt taaaagagat
     2581 aaagatggta tacctgctaa ggttgctact atagaatatg atccaaacag aactgcaaat
     2641 atcgcattat taaactatgc tgatggagaa aaaagatata tattagctcc agttggaatt
     2701 aacgttggag atactatatt atcaggatta ggtgctgata taaaaccagg aaactgttta
     2761 gcattaaaag atatgccagt tggtactatc attcataata tagaattaaa gccaggaaaa
     2821 ggagctcaat tagttagatc tgctggagtt tctgcacaat taatggctaa agaaggaaaa
     2881 aatgctttat taagattacc atcaggtgag atgagattag taagcataaa ctgtaaagct
     2941 actataggtc aagttggaaa tatagagcat ggtaacgtag ttatcggtaa agcaggtaga
     3001 aaaagacata tgggaataag acctactgta agaggttctg taatgaaccc taatgaccat
     3061 ccacacggtg gaggggaagg tagatctcca ataggtagac catctcctgt tactccatgg
     3121 ggtaaaccag ctcttggata caaaactaga aagaaaaata aagcttcaaa taaactaata
     3181 gtatcaagaa gaactaagta gtaaatttat ttttgctcgg aaaggaggaa tataaatgtc
     3241 aagatcaact aaaaaagggc cttttgtcca tgcaagactt ttaaagaaga tagaagctat
     3301 gaatgctagt ggaaataaag aagttataaa aacttggtca agatcttcaa ctgtatttcc
     3361 acaaatggta gaaaatacta tagctgttca tgatggaaga aaacatgttc cagtatatat
     3421 aacagaagat atggttggac ataaattagg tgagttcgtt cctactagaa ctttcaaagg
     3481 acataaggat gacgaaaaat ctaataagag aaaataatta aattatgact aagaaaggag
     3541 gaataacaat ggaagcaaaa gctactgcta aatacgtacg tgtatcacca agaaaagcag
     3601 gccaaatatg tgaccttgtt agaggaaaga atgttgatga agcattagca atattaaagt
     3661 ttactccaag aggagcagct tcaataatag ctaaagttgt aaaatcagct aaagctaacg
     3721 cagaaaacaa tcacgaaatg gatactgaaa aattatatat agcatcaata gttgctaatc
     3781 aaggaccaac aatgaagaga tttatgccta gagctatggg tcgtgcaact acaataagaa
     3841 aaagaacttc tcatatagag gttgttgtta aagagaaaaa ataattaata gataggaggg
     3901 aaagttaatg ggtcaaaagg ttaatccaca cggcttaagg gtcggtgtta taaaagattg
     3961 ggactcaaga tggttcgcta ctgataaaaa agagttcgga aacttattat tagaagacca
     4021 taatatacgt aaattcttaa agaaaagatt atactcagct ggagttgcta agatagaaat
     4081 agaaagatca gctaacaaaa taaaaatgga cttacatgtt gctaagcctg gtgtagttat
     4141 aggaagagct ggtgcaggaa tcgaagcatt aaaagctgaa ttagaaaaaa tgacaaagaa
     4201 aactataata gttaatatag tagaagtaag aagtacagat aaaaatgctc aattagtagc
     4261 tgaaaatata gctttagcta tagagagaag ggttgctttc agaagagcaa tgaaacaagc
     4321 tatacaaaga gcaatgaaat ctggtgctaa agggataaaa gtttctgctt caggaagatt
     4381 aggtggagct gaaatggcta gaactgaagg ttatagtgaa ggaaatgtgc cactacaaac
     4441 tttaagagca gatatagatt atggtttcgc tgaagctgat acaacttatg gaaaaatcgg
     4501 tataaaagtt tggatatgta atggtgaagt tttaccaact agagacggtg taaatccaag
     4561 agaagaaagc agaaagagcg atagaagaga caacaagaga gataatagaa gaaacgatag
     4621 aagaggaaat gacagaagag gaaatgataa cagaggaaac tacagaggac aaagacctca
     4681 aggtggatca agacctcaaa gaactgaaaa caaaggaaat taataatagt taggttcaag
     4741 gaaggaggaa aaatactcat gttaatgcca aaaagagtaa agcgtcgtag agttcataga
     4801 ggaagcatgg ctgggcaagc tcataagggt aataaagtta cttatggtga gtttggatta
     4861 gtagcattag aagcttcttg gataacttct aatcaaatag aagctgccag aatcgcgatg
     4921 actagatata taaaaagagg gggaaaggtt tggataaaaa tattccctca taaaccagta
     4981 acaagaaaac cagcagagac tcgtatgggt gcagggaaag gttctccaga atattgggta
     5041 gctgtagtta aaccaggaag agttatgttt gaattagcag gtgtttctga agataaagca
     5101 agagaagcta tgagacttgc tgcacataaa ttaccaatca aatgtaaatt tgttaaaaaa
     5161 gaagatttag aagtaaaggg tggtgaatag gatgaaagct aaagaattaa gagatttaac
     5221 aagcgaagag ctaatgaaca aattaaacga cttcaaaagt gaattattta gcttaagatt
     5281 ccaattagct actggtcaat tagaaaatac agctagaata aagtttgtta agaaggatat
     5341 agcaaaagtt aaaactgttc ttgctgaaag aaagttatac gaaactagag cttaatttgg
     5401 aaaggaggct ttttaaacat ggaaagagga agaagaaaag ttagaatagg ccgtgttgtt
     5461 agtgataaga tggataaaac aatagtagta gctgttgaag aattcgtacg tcatccacta
     5521 tataataaac gtgttaagag aactaagaaa ttcaaggctc atgatgaaaa gaatatatgt
     5581 aacatcggag atagagttaa aataatggaa actagaccat tatctaaaga taagagattc
     5641 agactagttg aagttgttga gaaagttaag tagtttttga agaaggaggg attacgatat
     5701 gatacaacaa gaatcacgtc taagagttgc tgataactca ggagctaaag aacttttatg
     5761 tatacgtgtt ctaggcggaa gtaaaagaag atatggtaac ataggcgacg ttatagttgc
     5821 tactgttaaa agtgcaacac ctggtggagt tgtaaaaaaa ggtaaggttg ttaaagctgt
     5881 tatagtaaga agcaaacaag gcgtaagacg taatgacgga agctatatat cttttgatga
     5941 aaatgctgct gttataataa aggacgataa aactccagta ggaactcgta tattcgggcc
     6001 tgttgctaga gagttaagag acaatgaatt catgaaaata gtttctcttg ctccagaagt
     6061 actataatta aggaggtgca ataggacatg atgcgtgtta aaaaaggtga cactgttgta
     6121 gttatagcag gtaaagataa aggtaaaaaa ggttcagttt taaaggtata tcctaaaact
     6181 agcaaagtgt tagttgaagg tgtaaatgta ataactaaac atcaaaaacc aagtgctatg
     6241 aaccaacaag gtggaataat aaataaagaa gccccaatac acatatctaa tgtaatgcca
     6301 tttgatcctg aaacaggaaa aggcgttaga gtaagatatg aagtaaaaga tgggaacaaa
     6361 gtaagagtat cagcaaagag cggaaaagaa ttataataaa tattgcttga aaggagggac
     6421 cttaaatggc ttctagatta caagaaaaat acatgaaaga agttgctcct gctttaatgg
     6481 agaaatttgg atacaaaaac gtaatggaga tacctaagtt aaataaaata gttattaaca
     6541 tgggtatagg tgacgctaga gaaaatccaa aaggattaga aaaaggcgtt gaagaattag
     6601 aaataatatc aggacaaaag cctgttataa ctaaagctag aaaatctgtt gctaacttca
     6661 aattaagaga aggaatgcca ataggaacaa aagttacatt aagagctgat aaaatgttct
     6721 actttatgga caaattagtt tcagtttctt taccaagagt tagagacttt agaggagtta
     6781 accctaatgc ttttgatggt agaggaaatt atgctttagg agttaaagaa caattaatat
     6841 tccctgaaat agaatatgat aaaatagata aagtaagagg aatggatata atatttgtta
     6901 ctacagcaaa gactgacgaa gaagctcgtg aattattaaa attattagga atgccatttt
     6961 ctaagtaagc aaaggaggga ttcccgtggc tagaaaagcg atggttgtta aacaacaaag
     7021 aaagcaaaag tacgctacta gagaatacac tagatgtact atatgcggaa gaccgcattc
     7081 agttttgaaa aaatttggta tatgccgtat atgctttaga gaattagctt ataaaggtca
     7141 aatacctggt gttagaaaag caagctggta gaagcttgat tagaatggaa ggagggcaac
     7201 aaaatgacaa tgacagatcc aattgcagat atgcttactc gtataagaaa tgctaatgtt
     7261 gttaagcatg aaactgtaga tgttccagct tctaatatga agaaagaatt agctagaatc
     7321 ttattagaag aaggtttcat aagaggttat gatgttatag aagatggaaa acaaggtatc
     7381 ataagaatac aattaaagta cggacaagaa ggcgaaagag ttataacagg tcttaagaaa
     7441 atatctaagc ctggaatgag agtttacgct gctaaccatg agataccaaa agtattaaac
     7501 ggattaggaa tatcagttat atctacttca aaaggaatat taactgacaa gcaagctaga
     7561 aaagaaaatg ttggtggaga agtaatctgc tacgtttggt aatcaatatg aacaatgtaa
     7621 ggaggtgcga ccatgtcaag aataggtgtt aagccaataa tagtaccagc aggtgttgaa
     7681 gttactatag ctgaaggaaa tttagttaca gtaaaaggtc caaaaggaac tttaactaaa
     7741 caattaagtg ctgagttaaa cataaaaaaa gaagaaaata caataatggt tgaaagacca
     7801 actgataata aaaaacatag atctttacac ggactaacta gaactttatt agataatatg
     7861 gttgtaggtg taaatactgg tttcgaaaag aaattagaat taaaaggtgt tggatataga
     7921 gctcaaaaac aaggaaagaa attagttatg aacttaggtt tctctcatcc agttgagatg
     7981 gaagatccag aaggaataac tgttgaagct ccaaaccaaa cagagttaat agtaaaaggt
     8041 atagacaagc aattagtagg gaactatgct gctaagataa gagcttggag aaagccagag
     8101 ccatacaaag gtaaaggtat aaaatacgtt gatgaagtaa tcagacgtaa agaaggaaaa
     8161 actggtaaaa aataatacca aaacttaaac gctagaaagg agtgagctag gtgttaaaaa
     8221 aggctgataa aaatgctaat agacttcaaa gacataagag agttcgtaga aaaataagcg
     8281 gtacttctca aagaccaaga ttatgtgtat ttagaagtgc taacaatata tatgctcaaa
     8341 taatagatga cacaaaaaga gtaactctag ttgctgcatc ttctttagaa gcagaagtta
     8401 agagtgctgt caatcataca ggaaacaagg aagcagctaa aaaagttgga gaattagttg
     8461 ctaaaaaagc tgttgaaaaa ggaatcactg aagttgtatt tgacagaggt ggatacttat
     8521 atcatggaag aattcaagaa ttagctgaag gtgctagaga agctggtctt aagttctaat
     8581 acgaaggagg gaaaaataca tgctacgtcg taagccgata gatgcaggac aacttgactt
     8641 acaagaaaaa gttgttgaag taagacgtgt tactaaggtt gtaaaaggtg gtagaaactt
     8701 cagatttgca gcattagtag ttgttggaga tgaaaacgga cacgttggta taggtgctgg
     8761 gaaagcaatg gaagtaccag acgctataaa aaaagcagtt gaagatgcta agaaaaatct
     8821 aatagtagtg ccaatagttg ggactacaat tcctcacgaa gtacgcggac actttggtgc
     8881 tggaaatata ttaataatgc ctgctgtgga aggtacagga gtaatagctg ggggacctgc
     8941 tagagccgta cttgagctag ctggactaaa agacgttaga gctaaatctt tagggtctaa
     9001 caatccaaga aatatggtaa atgctacaat agaaggactt aattctctaa aaacagttga
     9061 agatatagct aaacttagag gtaaaaaagt tgaagaactt ctagggtaag gaggtagtgt
     9121 aaaatggcta aattacaaat aaagttagtt agaagtgtta taggaactac tcctaaccaa
     9181 aaaaagaatg ttgaagcgtt aggattaaga aaaagagaac aagttgttgt aaaagaagac
     9241 aatgctcaaa caagaggtat gataaataaa gtaagtcact tattagaagt aactgaaata
     9301 gctgaataat ttttataaag tagtgcaagg aggtgcaatc catgaagtta catgagttaa
     9361 aacctgctga aggtgcagta agagctaaga gaagattagg tagaggtact gcaactggac
     9421 aaggtaaaac tgcaggacgt ggacaaaaag gtcaatggtc tcgttctggt ggtggagtaa
     9481 gagtaggatt tgaaggtgga caaatgccac tagctagaag acttcctaag agaggtttca
     9541 ataacatatt taagaaagtt tacacagaag ttaatgttga agttttaaac agatttgaaa
     9601 atggaacaga aataactgca gaattattaa aatctactaa aactataagc aaaataggta
     9661 aagatggaat taaaatctta ggtgaaggta atttagagaa agctttaact gttaaagctg
     9721 ctaaatttac agcttcagct caagaaaaaa tagaaaaagc tggtggaaaa gcagagttag
     9781 tataataacc tgctaatctc ctagccagta caggcggggt gaattagcgt gctgtcaaaa
     9841 ttaaaacaag cttggaagat taaagcagta agaaaaaaag taatgtatac acttatgatg
     9901 atcgtgatat tcagaatagg tactactata ccagttccag gcatagacac tagtattata
     9961 caaaagatgg taggcggcaa tagtctactt tctctgtata atatgtttac tggtggagct
    10021 tttagtaatt tttcactatt tgctttaggt ataagtccat atatcactgc atcaatcata
    10081 atacaacttc taacagtagg ttttgaaagt ttagcagaac ttcaaaaatc tggtgaagaa
    10141 ggaaagaaaa aaatcaataa gtacactaaa tatacagcac tagctctagc agttgtacaa
    10201 gctttgggaa ttacattagg aattgtaaga agtgctttaa tatcaaatag tgtgtttttt
    10261 atcacaactg ttgtaataac attagtttca gcaagtatgt tagtaatgtg gattggggac
    10321 aaaattactg aaaaaggaat aggtaatggt agttcagtaa taatatttgc aggtataata
    10381 tcaagaatac ctacagacgt tattaaaatc tcgcaacaag ttaaatctgg ccaagttgca
    10441 ccttgggtta tagtaattct agccgtagtt atattactta cagtaacagg agttacattt
    10501 atacaagaag caactagaaa gatacctgta caatatgcta aaagagttgt tggaagaaaa
    10561 atgtatggcg gacagagttc tcatatacca atgaaagtta accagtcagg agttatgcct
    10621 ataatttttg ctagttcact tttagctttc cctcaaacaa tagctatgtt tatgggtcca
    10681 aatgctcagg cttttgttca aaaatactta agtatggcaa cagaacaagg attttggaca
    10741 tatagatcaa ttgagattct acttataatt ttcttctcat atttttatac gactgtatca
    10801 tttaatacag aagacatatc taaaaatatg aagaataatg gtggatttat accaggtata
    10861 agaccaggtg aacctactat ggattattta aatagaattt taactagatt aactttagct
    10921 ggagctactt tcttagcaat aattgctatg gtgccagctc ttacaacaca ttatatgaaa
    10981 gtaaatatga gtctagctgg gacttcattg ctaatcgttg ttggagttgc tttggagctt
    11041 aagagacagt tagagtcaaa cttagttatg agaagctatc aaggtttctt aaaataaaat
    11101 ggagatggat aatatgagaa taatattact tggacctcca ggtgctggta aaggtactca
    11161 ggcggcagga atagtagaaa aatacaatat acctcatata tcaacaggag atatattcag
    11221 aaagaatata aaagagggaa cagaacttgg aaaaaaagct aaagaataca tggaccaagg
    11281 tttattagta ccagatgagt taactgtagg tttagttact gatagaatat ctcaagaaga
    11341 ttgtaaaaat ggatttatgt tagatggatt tccaagaaat gtagcacaag gagaacattt
    11401 agatatcttc ttaaaaaatg ctggtatatc actagataaa gttgtcaata ttgaagttga
    11461 taagagtata ctagtgtcta gagcagttgg tagaagaata tgtaagtctt gtggagctac
    11521 ttaccatgtt gagtttaatc ctcctaaagt agaaggtgta tgtgatgtat gccaaggaga
    11581 attatatcaa agagctgatg ataatgaaga aactgtatct aagagaatac aagtttatct
    11641 agatgaaact aagcctctag taaactatta tagcaagcaa ggtataatag cagatataaa
    11701 aggtgatcaa gcaatagata aagtatttga agatattgtc acagccttag gaagtggaaa
    11761 ataaatgatt atcttgaaat caaaaaaaga aatagaactt ttaagagaag caggcaaaat
    11821 agttgctgac actcatgaag ttttaagaaa agctatttct ccgggaatat ctactttaga
    11881 attagataaa atagctgaag agaatataag aaaatacaat gcagaacctt cttttaaagg
    11941 atatggaggt tttccaggaa gtatatgtgc ttctatcaat agagaggtag tacatggtat
    12001 tccaggagaa actatattac aagaagggga cattgtaagt ttggatatag gagcctatta
    12061 taaaggatat catggtgatt ctgcgaaaac tcatggagta ggcatgatat ctgaagaaga
    12121 taggaaattg atagaagtaa ctagagaaag cttctatgaa ggaataaaat ttgctaaatt
    12181 aggatataga ctttcagata tctcacacgc agtacaaaca cacgttgaga aacatggttt
    12241 ctcggtagtt agagatttag tgggtcatgg agttggagca aatcttcatg aggacccaca
    12301 agtacctaac tatggactcc caggaaaagg accaagactt agagaaggaa tggttattgc
    12361 tatagagcca atggttaatg caggtcgtta tcatgtaaag actctatcag atgggtggac
    12421 gactgttaca atagatggaa aaaaatcagc tcattatgag catacgatag caattactga
    12481 acatgaacct ttgatattga caaagttgta atttaaaaag taggtgaaaa aagtgctatc
    12541 agataattta agtataggtc aagttgttaa agtttcatta ggacgagata aaggaaattt
    12601 attctttgta gtcaaaataa ttaataatga atatgtatta atagctgatg gaaaaaagag
    12661 aaaacttgac aaacctaagt taaagaaagt gaagcactta aaaaaatata attttattaa
    12721 tgatgaagtt agaaaaagag tagtatctgg acaagagatt acagattctt ttttaagagc
    12781 tgaacttact aagttaaatt agataattta acttattgaa atggagggct ggttagttaa
    12841 tggccaaaaa agatgttata gaattagaag gtacagtttc agaagcttta cctaatgcta
    12901 tgttcaaagt taaactagaa aatggacatg agatattatg ccacatttct ggaaagctaa
    12961 gaatgaactt cataagaatt cttgaaggtg ataaggtgaa tgttgaactt tctccgtatg
    13021 accttacaag aggaagaatt acttggcgta agaagtagac tatattaata tttagtctaa
    13081 ggagggatta ataatgaagg ttagaccatc agtaaaacca atatgtgaaa aatgtaaagt
    13141 aataaaaaga aaaggaaaag taatggttat ctgtgaaaat ccaaagcata agcaaaagca
    13201 aggataagaa attattacta gcattttgtt aaattttata gtataatact atattgtgat
    13261 gttaacaaaa gaacgttaaa atcgttgaac aatctggcgt aggattggca tatgatgtga
    13321 agttttaaac actaaaaact atgtgtagga ggtgcgatca tggcaagaat agctggggta
    13381 gatttaccta gagaaaaaag agcggagata ggcttaactt atatatatgg tataggtaaa
    13441 gcgactgcta acgaaatatt agctaaggct gagataaatc cagatactag aataaaagat
    13501 ttatcagaag atcaagttaa tgagttaaga aaagttatag atgacgattt cttagttgaa
    13561 ggtgacttaa gaagagaaat tgctttaaac ataaagagat taagagatat caaatgttat
    13621 agaggaataa gacacgctaa aggtcttcca ttaagaggac aaagaactaa gactaatgct
    13681 agaactagaa aaggtcctag aaaaacagta tctcgtaaaa agaaaaaata aataaataat
    13741 aactagatga aggagggaaa aggaaaatgg ctaaaccaaa aaagaaagtt acacgtatta
    13801 gaagaagaga acgtaaaaat atagaacgtg gtcatgctca tatacaatca actttcaata
    13861 atacaataat aactttaact gacgttcacg gaaatgctat atcttgggca agttctggac
    13921 aattaggatt caaaggatca agaaaatcaa ctccatttgc atctcaaatg gctgctgaaa
    13981 cagctgcaaa agctgcgatg gaacacggac taaaaagtgt tgaggtattc gtaaaggggc
    14041 caggttcagg aagagaagct gcaataagag ctttacaagc aactggacta gaagtaacta
    14101 tgataaaaga tgttactcca atcccacata acggatgcag accaccaaag agaagaagag
    14161 tgtaattaat aagagtacaa taggaggtgt agataaatgg caagatatac aggtgcatca
    14221 tgtagacaat gccgtagaga gggaatgaaa ttattcctta aaggtgatag atgttataca
    14281 gataaatgtg ctatagtaaa aagaaactat gctccaggtc aacatggcca aggaagaaag
    14341 aaagtttcta actatggatt acaattaaga gaaaaacaaa aagttaaaag aatatatgga
    14401 gttttagaga ctcaatttag aaatttatat gaacgtgctg aaaatatgcc tggtaaagca
    14461 ggtgagaact tattaagttt attagagaga agattagata atgtagttta cagaatggga
    14521 ctagcatctt ctagaaaaga agctagacaa ttagtgactc atggtcattt cactttaaat
    14581 ggaaataaag tagacatacc ttcattaata gttaaggttg gagatgttat agaagttaaa
    14641 gagaaatcaa gatcttctgc aaaattcaaa aatttagtag aagttaactc aagaatagct
    14701 cctaagtggt tagaagctaa tgtagaagga atgacagcaa aagtagttgg tgttccaaca
    14761 agagaagata tagatcttga gatagcagag cacttaatca tagaacttta ctctaaataa
    14821 taagcacact tttaaaaatt attttaaaat tttgtttacc ctcagtggat tactgaattt
    14881 taaggagggt tttgtccatg atagaaatag aaaagccaaa agtagatata gttgagctta
    14941 gcgaagacta tagatatggt aaatttgtta tagagcctct agaaagagga tatggaataa
    15001 ctataggtaa cgcattaaga agaatattat tatcatcact accaggtgta gcagtgaacg
    15061 ctataaaaat agatggagtt cttcatgaat tttcaacaat acctggtgtc aaagaagatg
    15121 ttactgagat aatattgact ttaaaagagc tttcagcaac tatagatggt gaaggaagta
    15181 gaacacttaa aatagaagct caaggaccat gctctatcac aggtgcagat ataatctgtt
    15241 ctccagatgt agaaatatta agtaaagatt tagcaatagc tacattagat gataatgcta
    15301 agcttaatat ggagatattt gtagataaag gtagaggtta tgtttctgct gaagaaaata
    15361 aaacagagaa tgttccaata ggtgttttac ctgtggattc aatatatact cctgttgaga
    15421 aggttagtta tcatgtggaa aacacaagag ttggtcaaaa aacagattat gataaattag
    15481 tactagaagt ttggactaat ggtagtataa atcctcaaga gggtatatca ttagctgcaa
    15541 aggtactagt tgagcattta aacttattta tagacttaac tgaacatgta agtagtgttg
    15601 aaatcatggt agaaaaagaa gaagatcaaa aagaaaaagt tcttgaaatg actatagaag
    15661 aattagattt atcagttaga tcatataact gtttaaagag agcgggaatt aatacagttg
    15721 aagaattagc taataaatct gaggatgata tgatgaaggt tagaaaccta ggtaagaagt
    15781 cattagaaga ggtaatacaa aaactagaag aacttggatt gggattaaaa ccaagtgaag
    15841 aatagcgagc aaaggaggga taagaatggc taagtaccgt aaattaggac gtgaaacagc
    15901 tcacagaaac cttatgttaa gaaacttagt aacttgttta ctaagaagtg gaagaataga
    15961 aactacagtg actagagcga aagaaactcg tagaatggct gaaaagatga taactcttgc
    16021 taagagagga gatcttcatg ctagaagaca agttttagct tatgttatgg atgaaacagt
    16081 agttaataac ttattcacag atttagctcc aaaatatgct gagagaaatg gtggatacac
    16141 tagaataata aaaatagggc caagaaaagg cgatgctgct gaaatggctt ttatagaatt
    16201 agtatagtaa ggaaaggatt aggcttgatg gcttaatctt ttttttattt ttttgactaa
    16261 ggtatgatat aataatgtgt aaaatgattt ttataagtta ttgcttacta aaaggagtat
    16321 gaattaatgg ataatatagt aaaggtaaat aatatttcgt ttgagtatat tacagatgaa
    16381 gcaaaactta aagcaataga taatttaagc ctagatgtta aaaagggaga atttgttgcg
    16441 ataataggac ataatggttc tggtaagtct actttatcta aaaacttaaa tgctattctt
    16501 atgcctactg aaggaaatat tcttattgat ggtatgaata ctaaagaaga agaaagatta
    16561 tgggatataa gacaaactgc aggcatggta tttcaaaatc cagacaatca aatagttgct
    16621 actatagtag aagaagatgt tgcatttgga cctgagaact taggtataga accaaaagaa
    16681 ataagacgaa ttgtagagga gtctttaaaa agtgtaggta tgtatgattt aagagataga
    16741 cagcctcact tgttatcagg aggtcaaaag caaagagttg caatagcagg aattattgct
    16801 atgaggccta aatgtataat atttgatgaa gcaacagcaa tgttagatcc atctggaaga
    16861 aaagaagtta tgaaaacaat aaaaagactt aataaggaag aaaatataac tgttatacat
    16921 ataacacact ttatggaaga agcagtagaa gctgatagag ttgtagttat ggaaaaaggt
    16981 aagaaaatct tagagggaac tcctagagaa gtatttagta aaataaagat gttaaaggaa
    17041 atcggtttag atgtcccttg tatgacagag ttatctagtt tattaataga agaaggtata
    17101 aatattagtg gtgatatatt aactgtggat gagatggtga tggaattatg tcgattatag
    17161 taaagaactt aacacatata tataatgaag ggatgccttt tgcaagtaag gcacttgatg
    17221 atatttcttt tgagattaaa gatagagatt ttgttggtct cataggtcat acaggctcag
    17281 gaaaatctac attaatacaa catttaaatg gattactaaa gccttcctca ggagaaatat
    17341 ttataaatga ttttaatata acagataaaa acttgaattt aactgagatt agaaaaagag
    17401 taggggtcgt atttcaatat ccagaatatc aattgtttga agaaacgatt gataaagata
    17461 tagcctttgg accctctaat ttgggcttag aggagtccga gatacataat agggtaaaag
    17521 catcgatgga agctgtagga cttgattatg aggaatttaa agataaatct ccttttgaat
    17581 tatctggagg acaaaagcgt agagtagcaa ttgctggagt tatagccatg aacccagaag
    17641 ttctcatatt ggatgaacca actgctggtc ttgaccctgg tggaagagat gaaattttta
    17701 acttaataaa aagcttacat gaaaaaaaga atatgactat aatactatca tcacatagta
    17761 tggatgatat ggcaaaatta gcaaaaactt taattgttat gaatcatggt agtgtagaat
    17821 ttataggaac tcctagagaa gtatttaaat caaacgctag taaattaaaa gatataggat
    17881 tagatattcc acaagtttta gagcttgcat tgaaattgag agaaaaaggc tttgacatca
    17941 gtgaggatat cttgacttta gaagaagcca aacaggaaat attaaaagtt gtgagaggac
    18001 gaggattatg ttaaaagata taactatagg gcaatactac ccaacaagtt ctgctattca
    18061 taaattagat ccaagaataa agcttgtagc aacaattgta tttatgatat ctatatttgt
    18121 agtaaataaa ttttggcctt atatagtagt tttattatgt ttattagcta tgataaaatt
    18181 ggctaatata ccaatcaaat atattgttaa gggtgttaaa cctcttaagt ggattatttt
    18241 gtttacattt tttataaata tttttttctt accaggtgat gaaatatggt catttggatt
    18301 tttaactatt acaagccagg gtctaagaca agcaattttt atggcaataa gattaatatt
    18361 tttagtagtt ggtacatcat tgcttacttt aacaacttca ccaatagaat taactgatgg
    18421 aatagagaga ttgttgaacc catttaagaa aattggtttg ccagttcatg aacttgcaat
    18481 gatgatgaca attgctttac gttttattcc tacgttatta gatgagacag ataaaataat
    18541 gaaagctcag atgtctagag gtgcagattt tgaaagtaag aatcttataa atagagcaaa
    18601 gaatttagta ccacttttgg tacctttatt tgtgagtgca tttagaaggg ctgatgaatt
    18661 ggctatggcc atggaggcca gatgttacag aggtggacat aatagaacta agatgagaga
    18721 gtctgttata tcaagaagag attatatggc atgtgtattt caggttgtct atctaggagc
    18781 aattatagct actagattta tagcaattta attaataagt tatagatata aatgaggtaa
    18841 aatatgagaa atataaaaat tacaatccaa tacaatggta agaattattg tggatggcaa
    18901 aaacaacttg attctttagg gatacagggt acaattgaaa gggctatata tgatataaca
    18961 aaagaaaaag taagtcttat agggtctggg agaactgatt caggggttca tgctattggt
    19021 cagattgcaa actttaaaac taattcaagt ataagtatag aaagtatacc aatggcacta
    19081 aatgccaagt taccaaaaga catatcagtt atagatgcat gtgaagtaaa tgatgatttt
    19141 cactcaagat atagtgcaaa aggtaaaaca tataaatatt tagtatataa tagcaagttt
    19201 agaaatccaa tacttagtga gatatcatat caagtaaaat atgaacttga ttttgataaa
    19261 atgtgttcag aagcaaaact tttattagga acacatgatt ttaaaggctt tatgagttct
    19321 ggctcgtctg taaaagatac agttagaacg atatatgata ttgatataat tagaaaagat
    19381 gacttgatta cttttgagat tagtggtaat ggttttttat ataacatggt gaggattatt
    19441 gttggaacac ttgttgatat aggtcgtggt agaattaacg aaccattttt agatataata
    19501 caatcaaaaa ctaggtcaag atgtggacat acagctccag cacaaggatt atttctcaaa
    19561 aaagttcatt attaacttga cacaccaggc tacatgtatt aaaatatatg agaatgtgtt
    19621 aataagtcca cttgccccgg acttgacata aacggtaaaa aaattttatg aaaagtagtt
    19681 aaggagggac agttatgaaa agttatatag ctaagccagc tgatgtacaa agaaaatggt
    19741 acttagttga tgctgaagga aaaacattag gtcgtttagc aacagaaatt gcgacagtat
    19801 tgagaggtaa acataaacca acatttactc ctcacgttga tggtggagat tttgttgttg
    19861 tagtaaatgc agaaaaaata gttttaagtg gtaagaaatt agatcaaaaa tactacagat
    19921 accatactgg ttatgtaggt ggattaaaag aaatatctta cagagatatg atggataaga
    19981 aaccagagga agttatatca catgctgtaa gcggtatgtt acctaaaaat aaattaagaa
    20041 gtagaatgat gactagatta agagtatttg caggtgctga gcacactcat gcagctcaaa
    20101 atccagaagt tttaaacttt aagtaagatt aataggtgaa aggaggagtt tataatggct
    20161 aacgttcaat attatggaac tggaagaaga aaaagttctg ttgctagagt aagactagtt
    20221 gcaggtgaag gaaatatatt agtaaatgga agagcattag aaaattattt taactatgag
    20281 acattaataa gagatgttaa acaaccatta gttttaactg gtaatgaaaa taaatatgat
    20341 gttatagtaa aagttgaagg tggaggattc actggacaag ctggagctat aagacatggt
    20401 atatctagag ctttattaaa agctgattta gatttaagac ctgctttaaa gaaagaaggt
    20461 ttcttaacta gagatgcaag aatgaaggaa agaaagaaat acggattaaa agcagcaaga
    20521 agagctccac aattctcaaa aagataatta cttatttttt tggaaaaaca aaatccttta
    20581 tttactgcta ttcagaaaat attggtattt taaaaatgtt gggttaagtt aattattaat
    20641 atataataaa ataataagat atcttattag gatgtttttc attaagaaaa gcatcctttt
    20701 tattttgact atctaataaa gaatatatgc taataatatt tagattttat ttttataata
    20761 ttgtgattta aaagtttaca taagatgata tattttaaga tattatttaa caatataaat
    20821 tatgaatata tcttatcaaa gtttataata taaactaaat ataatttatt gtatagtatt
    20881 gaatgttaga taaatatagt gagttaaaga ggggtaggga catagtatca ttacataata
    20941 atgtattaat actaataaat agatatatat aaatattaaa agaaaattta aatgtttgta
    21001 ctaatatttg gaatattata gtgtttttat aagtatacat atacagggtg atgttgtgag
    21061 aaagtacata aaacatataa tttttagttt tgcaatgata tgtctagtag tagtatccat
    21121 atttgaaatt aaaaatattt ctgaagatgt tatcaagtat atgccagtaa caaacaaaac
    21181 tataattctc gatgctggtc atggaggtat tgatccaggc gcattaaata aggataagag
    21241 tacatctgaa aaggatatca acctagcaat aacacttaag cttagagagc ttatagaatc
    21301 aagtggtggt cttgtaatat taactcgaga ggatgacagt agcctttata aagaagaaaa
    21361 taataaaact acaagacaaa aatataatga gaatttgaag aatagaaaag aaataatatc
    21421 taattctaat gcaaatatgt ttgtctctat acatcttaat gcatttgagc aatcaaaata
    21481 ctatggagct caaacttttt atcctaaaga taagcaagat agtaaagaat tatcgaagtg
    21541 tattcaagaa gaacttaaaa gagtggtaga taaaacaaac aatagagaag tcaaaccgag
    21601 ggatgatata tatcttttga aagaaaataa tattccatca gtattgatag aatgtggttt
    21661 tttatcaaat gaaaaagaga gtaaactttt aactgatgaa acatatcaag aaaaaatagc
    21721 atgggcaatt tacataggaa tacaaaaata tttaagttga aataactata aaaataggtc
    21781 taaatatgta aatagcttgt attaaagcag ttaaaatgca ttaatatagg ctatttttat
    21841 tttgacaaaa aaatatttaa aataaaagtt aaaaaatagt tgacttagaa tggtatagat
    21901 gatattatat atgagtgccc aaaaggagca ccaaaataag ac
//
LOCUS       DAFNNB010000054        21469 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0054, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000054 DAFNNB010000000
VERSION     DAFNNB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0054"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(389..1822)
                     /locus_tag="L3M60_001760"
     CDS             complement(389..1822)
                     /locus_tag="L3M60_001760"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003243232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBN6226435.1"
                     /translation="MKSNQEFLWGGAVAAHQVEGGYNKGGKGISIADVMTAGTHTISR
                     KVTDGVIEGLNYPNHEAINFYENYKEDIRLFAEMGYKCFRTSIAWTRIFPNGDESIPN
                     EDGLKFYDDLFDELLKYNIEPVITLSHFEMPYHLVKNYGGWRNRKLIDFFVNFCEVVM
                     SRYKDKVKYWMTFNEINNQSITTNPIYAFTNSGIIYEEQEDKEEVMYQAVHYEFVASA
                     KVVKIGHEINPEFKIGCMVAAIPSYPYSCNPEDMIKFVESNREQLMFTDVHVRGHYPR
                     HTLKLWERKNYNLDITDEDKKILKEGTVDFIGCSYYLTTVVSADKTMKTTGNDSAGKA
                     DVVENPYLKTSDWGWNIDPVGLRFYLNQLYDKYELPIFIVENGFGAEDVLKSDNTVDD
                     GYRIEYLASHIREMKKAIEIDGVDVIGYTVWGCIDPVSFTTGEMKKRYGFIYVDKNND
                     GSGTLKRYKKKSFDWYKNVIKFNGEIL"
     gene            complement(1845..3686)
                     /locus_tag="L3M60_001761"
     CDS             complement(1845..3686)
                     /locus_tag="L3M60_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6226436.1"
                     /translation="MKFERESEEIIKYVGGKENIISLVHCATRLRFKLKDTSKTNKEE
                     LLKMKDVLSVVNSGGQYQVVIGNKVTDYFETITKKAVLQVQNTGEQGEKVSIVSRIFE
                     SISGAFSPLIPALAGAGMIKALLTILTIMKWMSDTSSTYAILSAAGNAVFYFLPIFLG
                     ITLAIKLGANPYVGGVIGAALLDPNFTALIGAKGGANFFGMPVTAIDYSFGVFPIFVA
                     IFIYYYVDKLLKKIILKDLQLFLVPMISLLVMVPLTVILFGPIATNVGDVISNIFMGL
                     FEFNRVIAGAAIGIAMPFLAVLGLHWGFTPITLQNLATMGGDPIEGAAVAAVFAQIGI
                     AIGLFMKVRKSKKNSELKVLAGSTAVTGILAGVTEPIIYGIILRYRRMIPVLAIAGGI
                     GGGICGVFGVTCNAYVFHNIFSMPVYTPFLGYILGIGTALVLGAVLSYFFGLSKEECE
                     EGNEEVAFDEDDGIEEILSPIEGLQIELSEVEDEVFASGTIGNGMAIIPSKGEVVSPV
                     DGTITTIFPTKHAIGITSDKGVEILIHVGLNTTMLDGKYYITHMNAGDKVKKGDKIVS
                     FDKDKIEGEGYSIVTPIILTNTNDLADVIVKENKHIKIGTPVIKVVY"
     gene            complement(3812..4645)
                     /locus_tag="L3M60_001762"
     CDS             complement(3812..4645)
                     /locus_tag="L3M60_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6226437.1"
                     /translation="MVIKKVFNNNVVFARDNSGQEVILMGKGIGFNKFTKDLVEIDKI
                     EKKFIFNNEESLSELHSLLDRIPLKDVELVSEIIQMGRDELGDNLNDSILITLSEHIS
                     YMLKRMDEGLVFQSPLQWEIRQIYPNEYKFSKKAVEYLRKKLDKDIPDSEIAFITIHF
                     ANAQLETKDMQETLLLTKIIDNVLDIIKYHYGIEINEYSFDYTRFISHLRCFIKRQLS
                     GENANVDTSLLDIIKMKYKSDYNCAKKVKNFLEKTYKWKISENELLYLTLHLNRLSNI
                     K"
     gene            complement(5211..5456)
                     /locus_tag="L3M60_001763"
                     /pseudo
     CDS             complement(5211..5456)
                     /locus_tag="L3M60_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5683..5895)
                     /locus_tag="L3M60_001764"
     CDS             complement(5683..5895)
                     /locus_tag="L3M60_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226438.1"
                     /translation="MAILMKTKIKEYREKFLMTQNELAKLVGVRRETIVHLENGKYNP
                     SLKLAMDIAKVFDTTVENLFEFIEQE"
     gene            complement(5896..6312)
                     /locus_tag="L3M60_001765"
     CDS             complement(5896..6312)
                     /locus_tag="L3M60_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226439.1"
                     /translation="MSKSETGVKSKLILELLVITYFFIAVLATKYIFKGPSTASLPGL
                     SWLIVFGCYSIYNKKYNDLVDESSEFILSKVNKIAIKIMIYSIVIISIFFATPYTSNI
                     NISNIDTGIILIAVLYIQSLSKLVLYIYFDKRGICN"
     gene            complement(7620..8249)
                     /locus_tag="L3M60_001766"
     CDS             complement(7620..8249)
                     /locus_tag="L3M60_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HBN6226440.1"
                     /translation="MKNRKVYMYVFDTMSDWEVAYLTAELNTGRYFKKGLKPLKVITV
                     GIDKNPIITMGGLKVLPEITVDEFNMESKDLLILPGGNTWLSMNHEIILEKAKEALIQ
                     DSIVAAICGATLGLAKKGLLDFRNHTSNDLEFLKMVVPNYSGEKYYKMESVVSDEHLV
                     TAPGIAPLEFTFQVLKLLEVFESEALNSWLNLYKTHDSKYFFELMNCNK"
     gene            complement(8328..9290)
                     /locus_tag="L3M60_001767"
     CDS             complement(8328..9290)
                     /locus_tag="L3M60_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017561613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBN6226441.1"
                     /translation="MAKIDNMMSILWMLSSDKKITAKEIAEKLEINIRTVYRYIDVLS
                     ASGVPIISDIGHNGGYSLLNNFTKAPLFFDIEEQKSLLHAALFAKKEGYFLDEALDKS
                     ILKLKMYSNQKQEDILTNHLIALEVIKPIGKVSIESVLKKLENSILKELSVEINYHAG
                     RKAKSKYRIINPYGIVHWNNNWYVVAFCNMRNEIRSFRVDRISEIIETSNTFNRPTEF
                     SASEFFIKGIIPEIKDEQSIIQLVVEGSIDSLDSLSQHWFLGHYLKERTPNKIIFMLE
                     EEVICKYIPNILLPYRKTIQVIQPLSLRKKMIEDLLELIDYYQI"
     gene            complement(9662..10100)
                     /locus_tag="L3M60_001768"
                     /pseudo
     CDS             complement(9662..10100)
                     /locus_tag="L3M60_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            complement(11015..14491)
                     /locus_tag="L3M60_001769"
     CDS             complement(11015..14491)
                     /locus_tag="L3M60_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226442.1"
                     /translation="MQQKCKKKALSMILSISMGLGSVASFNVSNTFADTIEESTNAGN
                     VEGDTNAGNVEGDTNAGNAEGDTNAGNAEGDTNAGNAEGDTNAGNAEGDTNAGNAEGD
                     TNVGNAEGDTNVGNAEGDTNAGNAEGDTNEGNAEGDTNEVLVEEDTNAEEYDSVGDFK
                     ISGGEINVDFKFESGVLTILSGKQMTIQNNNPNTPTTHRIVIPKDVKAELIFNGVNIK
                     TASNSPFTLTPDKDGGAYAHIILQDGSKNILKSGDQLYPGIRAGKSTTLIIDDSVVNR
                     DIEGEFIVPENGKIPRDVTLENGDKLKKGDRLTLLDSKNRGSLTAVGNSYAAGIGGGD
                     TENGGNIIINGGIINATAMEGYNTGFGAGIGGGSIGDGGDITINGGTVTAKGAYHGAG
                     IGGAYYISGNHTLSPSVVDRNKGGGYSGNITINGGLCYSYGGAHGSAFGDGCVNPGAN
                     RGYRVLVTGGTVLPYMTGAGDWIFDLSAITGDVIVTGGSLKATTFKSSGETVAYGDLE
                     KKTKVFMSKVSLKYLGQDKVNTKLVDDFTMQINGKEYPYGAPSYTDDQGVLYLWFSDK
                     FTGSEVSVKLAIVDKDTGDTLKSDTFYIKDIGPTTPFLKQYEEFIIDEINTPPGSINK
                     RYDGLPFGKEQEKAFLDWILKDGIPVTNPKGGKLTDGTYIIISSQRLNEETMEPDTSL
                     GSDDLTKQVDVGKYQLIITSKQYASVAPFNESYWGHRSHYNYAEITPADTETSLVVET
                     PKVKTDVFKPTDTFTLNATVSPDKKEGPDCASPKGYVQFYINGKEYKDPVELKTHDKV
                     SESHNYSTASIEWKPLDSNHHILNTEQTITVKYIGDDINYTKDSKDEKKLKLDITNVD
                     IDGDGIPDINIDTDGDGRPDINIDTSGDWKPDINIDTDNTGEWKPSTEGGNRDGIWKP
                     DKNIDTNGDGNPDTDYNRPAIDTDNDGVDDYWKPDKNVDTDSGGYDTGNPDPNKPDPD
                     NPNPDNGGNSKPDSGNGGNSKPDSDNGGNSKPDIDTDGDGKPDIDGDGKPDIDIDTDG
                     DGKPDINIDTDGDGKPDINIDIDGDGKPDINIDIDGDGKPDINIDIDGDGKPDINIDI
                     DGDGKSDVNIDIDGDGKPDINIDTDESLDSNAGQNVQTGDQSNIMLDLALMFTSLFFL
                     IKNLINKYLRRK"
     gene            14761..15295
                     /locus_tag="L3M60_001770"
                     /pseudo
     CDS             14761..15295
                     /locus_tag="L3M60_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427525.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
     gene            15494..16657
                     /locus_tag="L3M60_001771"
                     /pseudo
     CDS             15494..16657
                     /locus_tag="L3M60_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(16795..17394)
                     /locus_tag="L3M60_001772"
     CDS             complement(16795..17394)
                     /locus_tag="L3M60_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226443.1"
                     /translation="MSQNIDFNKSNFYLRLDERLAVPLKYAYVSTILKFQKWRGCTFD
                     FSNTKLEKLKNIIVHGDNDNYNKLLMVIKKRYDDILDEEFWHIEIKELPYIDRGFKSN
                     KDIITTFIHGKYYPKRKAFRHIDFIKNQYPFKEYCSKQEDNSTNEIKIDFYITKECHY
                     KVWCIENIDIQEHIWCELVNVSLSEEYRKLFGEILDSSK"
     gene            complement(17420..17719)
                     /locus_tag="L3M60_001773"
     CDS             complement(17420..17719)
                     /locus_tag="L3M60_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226444.1"
                     /translation="MAYSDIYSISCGEKEFPTNITIDEDLLFCVVTCFVLDRFRYDDL
                     NIKSVISYEKHMYDINDYKLTKIENLDFKQNGFIYDKKYYLYNIFIDKSPLNKYK"
     gene            complement(17747..17998)
                     /locus_tag="L3M60_001774"
     CDS             complement(17747..17998)
                     /locus_tag="L3M60_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBN6226445.1"
                     /translation="MEKLIQDIKNLDSYYNTSYYTFHLDEPLWKSLKVRNWSGVPYYI
                     KQLCNEVKSLIKERNLTQSEIFQYITDPKKRTHYFFVAQ"
     gene            complement(18213..20144)
                     /locus_tag="L3M60_001775"
     CDS             complement(18213..20144)
                     /locus_tag="L3M60_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6226446.1"
                     /translation="MFLDRIIMENVGPIDNLDIGFPFNEDGSPKPIIFVGENGKGKST
                     VLSNIVDSLFEFAKQPYNDITETNKDGTTPYFKVLSWKNTKVGCDYSFSFINFVNGEQ
                     NFQYIEKLGNLSFEQCIEKTSGLLNLDNNWGNRKNIKDITKNKEYFEEEFANKSFCFF
                     PPNRYETPHWMNQNIISENLITINDKISGKLNKPIIVQNVTETNIKWLLDIIVDSRAD
                     LIQSDLGGWNSEQDLNNIRLLKIARENVEEILSEIVKKDVKFGLNWRNSGGSRFNIID
                     RSTNSMIIPTLDALSTGESALFNMFSTIIRYSDYGDINKSIKLNEIEGIVIIDEIDLH
                     LHSSIQCDVLPKLIKKFPKVQFIITSHSPLFILGMKDIFKEDGFKIYEMPTGENIGPE
                     EFSEFERSYEYYKRTELFSKDIQDILKSKNGKTLIITEGKTDWKHMKAGLLKLKEQGL
                     YHDLDIEFLEYENDIKMGYNNLINICEAASKLRNNNKVICVFDRDLSDRDLRKVKDEN
                     TNYKLWGNNVISMVLPLPDNRVEIDKIMCIEHYYSDEEIKTFYKGRRLFLGDEFNSKL
                     GLHILGDKICQDKNKCGKKSIIDSGCVVCDIKNPEDNIALSKNDFADSILNGVEEFKN
                     ISFENFKLVFDLIQILVNE"
     gene            complement(20219..20568)
                     /gene="ssrA"
                     /locus_tag="L3M60_001776"
     tmRNA           complement(20219..20568)
                     /gene="ssrA"
                     /locus_tag="L3M60_001776"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(20726..21181)
                     /gene="smpB"
                     /locus_tag="L3M60_001777"
     CDS             complement(20726..21181)
                     /gene="smpB"
                     /locus_tag="L3M60_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBN6226447.1"
                     /translation="MAGKKILATNKRARHEYFIEETYECGIELKGTEVKSIRQGKVNL
                     SDGFASVDNSEVFLKQVHISPYEQGNIFNVDSLRVRKLLLHKHEIRKLIGATTIKGYS
                     LIPLSLYLKNGKVKVEIALAKGKKLYDKRQDMAKKDAQRRIEREMTGKY"
BASE COUNT         7173 a         3711 c         2196 g         8389 t
ORIGIN      
        1 actaatttag atttagtatg cttataataa gtatctttca tttcacagtc tataaatata
       61 ttagtttctt caccattaat aattactaaa aaactatctc cacaataatc ttaaaacatt
      121 gtaattttat actagttcat atattaattt tctctaaaag ctaaataaat ttgtataata
      181 ttctatatat taaatcatat gtatcccaat ttttaacttt acctcttcaa atttatattc
      241 atttttaata aaaaaatatt gactatttag tcaatatctt ttagtagatt taaatggtaa
      301 agactatcct gagaatcata cccacgattt taatctaatt tttaatggtt tccttagcat
      361 aatcttataa atattaaaat ttatctactt ataaaatttc tccattaaat tttattacat
      421 ttttatacca atcaaaagac ttctttttat atcttttaag agtcccactt ccatcattat
      481 ttttatctac atatataaat ccatatcttt ttttcatttc tcctgttgta aatgacactg
      541 ggtctataca tccccaaaca gtatacccta taacatcaac tccatcaatt tcaatggcct
      601 ttttcatttc ccttatatga ctagccaaat actctattct ataaccatca tccacagtat
      661 tatctgattt taatacatcc tctgctccaa atccattttc aacaataaat ataggaagtt
      721 catacttatc atataattga ttaaggtaaa atcttagacc tacagggtct atattccacc
      781 cccagtcaga agtttttaag tatggatttt caactacatc agcctttcct gccgagtcat
      841 tacctgttgt tttcatagtt ttatcagctg atactacagt agttaaataa taactacatc
      901 caataaaatc aactgttcct tcttttagga tttttttatc ctcatctgta atatctaaat
      961 tataattttt tctttcccat aattttagtg tatgccttgg ataatgacct cttacatgta
     1021 catcagtaaa cataagttgt tctctattag attctacaaa tttaatcata tcctctggat
     1081 tgcaagaata aggataactt ggtatagctg ccaccataca tccaatttta aactctgggt
     1141 ttatttcatg acctatcttc acaacctttg cacttgctac aaattcataa tgaactgctt
     1201 gatacataac ttcttctttg tcttcctgct cttcatagat tattcccgag ttagtaaatg
     1261 cataaatagg atttgtagtt atactttgat tatttatttc attgaaagtc atccagtact
     1321 taactttatc cttgtatcta ctcattacca cttcacagaa gtttacaaag aaatctatta
     1381 attttctatt cctccatcca ccatagtttt tcactaaatg ataaggcatt tcaaaatggc
     1441 ttaaagttat tactggctct atattgtatt ttaataattc atcaaataaa tcatcataaa
     1501 atttcaatcc atcttcattt ggaattgatt catctccatt tggaaaaatc ctagtccaag
     1561 caatacttgt cctaaaacac ttatatccca tttcagcaaa taatctaata tcttctttat
     1621 aattttcata aaaattaatg gcttcatgat taggataatt taagccctca attactccat
     1681 ctgttacctt tcttgaaata gtatgagtac ctgcagtcat cacatctgca atacttattc
     1741 cttttcctcc tttattatat ccaccttcaa cttgatgggc agcaactgcc cctccccata
     1801 aaaattcttg attagatttc atagaaatat ctcctccagt ataattaata aacaactttt
     1861 attacaggtg ttccaatttt aatatgttta ttttctttaa ctattacatc tgctaaatca
     1921 tttgtgttgg ttagtattat tggtgtaaca atactatacc cttcaccttc tattttatcc
     1981 ttatcgaaag aaacaatctt atctcctttt ttaactttat ctcctgcatt catatgagta
     2041 atataatact ttccatctaa catagtagtg ttaagaccta cgtgtattaa tatttctaca
     2101 cctttatcag aagtaattcc tattgcgtgt tttgtaggaa aaatagttgt tattgttcca
     2161 tctacaggag atacaacttc tccttttgaa ggtattattg ccatgccatt acctatagtt
     2221 cctgatgcaa acacttcatc ttctacttca gataactcta tttgtaagcc ttctattgga
     2281 gataatattt cctctatacc atcatcttca tcaaaagcaa cttcttcatt accttcttca
     2341 cattcttctt tactcaatcc aaagaaatat gacaatactg ctcctaacac tagtgctgtt
     2401 cctattccta atatatatcc tagaaatggt gtataaactg gcattgagaa tatattatgg
     2461 aatacatatg cattacaagt aacgccaaat acgccacata taccaccacc aatacctcct
     2521 gcaatagcca atactggaat catccttctg tatcttaata taattccata aattataggt
     2581 tctgttactc ctgctaaaat tcctgtaact gctgttgaac ctgctaatac ttttaattct
     2641 gaattttttt tagattttct aaccttcata aataaaccta ttgcaatacc tatttgagca
     2701 aatactgctg caacagctgc accttctatt gggtctccac ccattgtagc caaattttgt
     2761 aaagtaattg gcgtgaagcc ccagtgtaat cctaatactg ctaagaatgg catagctata
     2821 cctattgcag ctcctgcaat tactctatta aattcaaata atcccataaa aatatttgat
     2881 attacatccc caacattagt tgctatcggt ccaaataaaa ttacagttaa aggaaccatg
     2941 actaataatg atatcattgg cactaagaac aattgtaaat cttttaatat aatcttcttt
     3001 aaaagcttat caacataata ataaataaat atagcaacaa atataggaaa gacaccaaaa
     3061 gaataatcaa ttgctgttac tggcattcca aaaaagttag caccaccttt tgctccaatt
     3121 aaagctgtaa aattaggatc taacaaagct gctcctatca ctccaccaac atagggattt
     3181 gcaccaagct ttatagctaa agtaatacct aagaatattg gtaaaaagta aaatacagca
     3241 tttccagctg ctgataatat agcataagtg ctacttgtat cagacatcca tttcataata
     3301 gttaatattg taagcaatgc cttaatcata cctgctccag ctagtgcagg tattaatgga
     3361 gaaaatgcac ctgaaattga ttcaaatatt ctagaaacta ttgaaacttt ttctccctgt
     3421 tctccagtat tttgaacttg taaaacagct ttttttgtaa tagtttcaaa gtaatcagtt
     3481 actttatttc ctattactac ttggtattgt cctccactat ttactacaga tagtacatcc
     3541 ttcattttta ataactcttc tttgttagtt tttgaagtat ctttcaactt aaaacgtagc
     3601 ctagttgcac aatgaactaa actaataata ttttcttttc ctccgacata ttttataatc
     3661 tcttcagact ctctttcaaa tttcatacaa tctatcctcc attttcataa ttttatgtat
     3721 aaaaatagac ctaaatcaat acaattagat gttaaaaact aattatacca acttaggtca
     3781 tgcctgccaa acagtaacaa tcctacaata tttactttat atttgataat ctatttaaat
     3841 gcaatgttaa gtacaacaat tcattttcgc taattttcca tttatacgtc ttttcaagaa
     3901 agttttttac tttttttgca caattgtaat cacttttata tttcattttt attatatcta
     3961 acaatgatgt gtctacattt gcattctcac cacttaattg cctttttata aaacacctta
     4021 aatgacttat aaaccttgtg tagtcaaatg aatattcatt tatttcaatt ccatagtgat
     4081 actttatgat atctaataca ttatcaatta tcttagttaa caataaagtt tcttgcatat
     4141 cttttgtttc taactgagca tttgcaaagt gtatcgtaat aaatgctatt tcagaatcag
     4201 gaatatcttt atctagtttt tttcttaaat attctacagc tttcttacta aatttatatt
     4261 cattaggata tatttgtctt atttcccatt gaagtgggct ttgaaatact aatccttcat
     4321 ccattctttt taacatgtaa cttatatgtt ctgataatgt gattaatata ctatcattaa
     4381 gattatcacc caattcgtct cttcccattt gaataatttc acttacaagc tcaacatctt
     4441 ttaaaggtat cctatctagt agtgaatgta attcacttaa cgattcctca ttattgaata
     4501 taaatttctt ttcaatttta tctatttcaa ctaaatcttt agtaaattta ttaaatccaa
     4561 ttccttttcc cattaatatt acttcttgtc ctgaattatc tctggcaaat acaacattat
     4621 tattaaatac tttctttata accataaact acctccaatg taaacaaaaa gagacctaaa
     4681 aaagactaat aatactttaa gcatttacta gaatttttag gtcacgcctg attacttcta
     4741 tctcagtaac gatccgttta tttatctgta tgataacttt attttcatta taaattaata
     4801 actgtactta gtcaatatat aattcatgta tatatctacc tatattattt tcttcaatat
     4861 tcactcattc tacttttttt catataattt atataaccta aacatgctaa aataagtttt
     4921 gaagttattt cataacaaaa ttaaactcat atattgttta ctatatatgc tcgattattt
     4981 atcaatatat aaggaaatcc tttatttttg taactacaat aactgactaa gttttgattt
     5041 tcttttatct tttacttctt attattaaaa aataattctt ttactgttgt atctaaagct
     5101 ttaaacagtt taatcatcaa atcattgttt ggattattaa ccttacctaa cttactatat
     5161 agctttttaa ctgcatatct ttaatagacc ttttggtatg ctaatccatt ttataatatt
     5221 aattcttgaa tcaagcatat attaccctta caatgcatat cttataccct ctgtaaagtt
     5281 attcctgaag aaattactct tacaagacat atctgataca acctcactta taagtttata
     5341 tgtttcagaa gtatataaat aacccatact tccttccaaa ctcttacctg ttttcttaga
     5401 aaattctaat agatttcctt acataacaat tgtaacagag tcttattaga tttcataaaa
     5461 ctatacatcc tctttacatt taaactttaa acttttatct attgtttatc aatcaaatgt
     5521 atattcacta ttttttatca tttataatac tcttcttctt atcttaaaac aattataatc
     5581 taaccttaat cacatactat attatataaa tatttttaac aaacttaata attaaataac
     5641 tatattttat tatccatctt ataaatgttt ataaactttc cactattctt gttcaataaa
     5701 ttcaaacaaa ttttctactg tggtatcgaa tactttagcg atatccatag ctaacttcaa
     5761 agaaggatta tacttcccat tttctaagtg tacaatagtc tcacgtctta ccccaactaa
     5821 tttagcaagt tcattttgcg tcataaggaa tttttctctg tattctttaa tttttgtttt
     5881 catcaatatt gccacttagt tacatatccc ccttttatca aagtatatat acaacactaa
     5941 tttagataaa gactgtatat ataaaactgc aatgaggatt atacctgtat ctatgtttga
     6001 tatatttata ttacttgtat aaggtgttgc aaagaaaata gatattatta ctatagaata
     6061 tatcattatt tttattgcaa ttttattaac ctttgatagt ataaattcag atgattcatc
     6121 gactaaatca ttatattttt tattataaat tgaataacat ccaaaaacta ttaaccaact
     6181 caaccctgga agagaagcag tgcttggacc tttaaaaata tacttagtgg ctaatacagc
     6241 tataaaaaaa taagttatta caagcaactc caggataagc tttgatttta caccagtttc
     6301 tgatttagac atataaaatc actccttaaa aattatattc tctatactct ttaattttcg
     6361 tttttatcaa tattatcact tagttgcata ttcccttttt atcaaaatat atatacaaaa
     6421 ttaatttaga taaagattgt atatataaaa tagctaagag aattacacct atatccaaat
     6481 ttgatatatc caaacctctt gtataatttg cttaaaagaa aacagctatt agaaaaatag
     6541 aatataacat tatttttgat gcaatatgat caacctttaa taacataagt tttgatgcct
     6601 catcaactaa atcattatat tttttattat acatgaagtc acatgcccca aaatcgaata
     6661 aaattataaa agtaattcaa atatttcccc ctttaaaata aactttttta ataatgaatc
     6721 tataaaaaat aggatatcat aagtagcttt gaaacaagat ttgttttaaa tccaagctct
     6781 gatttagaca tataaactcc ttaaaagtta tatatttata acaccttatg ttataaatat
     6841 ataacttttc caaataaatt gcaatagaat atcgtaaata ttcaataata tctttataaa
     6901 tatagtttat aaaggatttt ataataaaca ttgcaataca gtactaaatt agtttattgc
     6961 atatattatt tcaatcatat catttactaa ctattattct aatgagcata taacattgaa
     7021 atttataatt atacaaccta aatgcatccc aatgtaccct caaaaccttc attttctgta
     7081 actgaagtat aattggtaga ttttatcact gaaataaaaa acactagtca ataaagacta
     7141 gtattttcaa gtattttatc attctttttt ttaattcttg gagaagaatc atcagaataa
     7201 tacctacaat ttatctatat gcaaattcta aatttaaaat aaagtttcat atgaatttac
     7261 tattctatgc tatgatacaa cagaaaaaac tacaatctat gagcggtagc ttcatataat
     7321 cctagttcct acagtaatca aaaaggaagt ttattctata tgtaactaat gataaattct
     7381 attataggag ttattagctc tatttgtgtt actataagta gtaattatat atataataat
     7441 tttttaacac ttttctattg gctaaataga aagtagttta gaacttaaat ttaaataaaa
     7501 tttaagttca ccaaaagaaa tctatattag aaaccactac tctattccac cagattgtaa
     7561 ttttttgttc tttatatcat aatgctatac cacattaaat ttaaaaataa attttaaatt
     7621 tatttgttac aattcattag ttcaaaaaaa tactttgagt catgagtttt gtaaagattt
     7681 aaccatgaat ttaatgcttc tgattcaaaa acctctaaaa gtttcaatac ttgaaacgta
     7741 aattccaacg gagcaattcc tggagctgta actaaatgtt catcactaac tacagattcc
     7801 attttataat atttttctcc agaataattt ggtactacca ttttaagaaa ctctaaatca
     7861 ttacttgtat gatttctaaa atctaataga ccttttttag ctagacctaa tgtagctcca
     7921 catatggctg caactataga atcttgtatt aacgcttctt tagctttttc taatattatc
     7981 tcatgattca tagataacca agtatttccc cctggtaaaa ttaataaatc cttactttcc
     8041 atattaaact catcaactgt aatttcaggt aaaactttta acccacccat tgttataata
     8101 ggatttttat caattcctac agtaattact tttagtggtt ttaatccttt tttaaaatat
     8161 cttcctgtat ttaattcagc agttaaatat gccacttccc aatctgacat agtatcaaat
     8221 acatacatat atacttttct atttttcatc ttttttactc cttatgtaat taatttatat
     8281 atctaattgt aaaataactt aactgacata ttcagtcagt taagttatta aatttgataa
     8341 taatcaatta attctagtaa atcttcaatc attttcttcc taaggcttag tggttgaatt
     8401 acttgtattg tctttctata aggtaaaagt atatttggta tgtacttgca tattacttcc
     8461 tcttcaagca taaatattat tttattaggt gttctttctt tcaagtagtg ccctaaaaac
     8521 caatgttggc ttaaactatc taaagaatct atactccctt ctacaactaa ttgaattata
     8581 ctttgttcat cttttatttc aggtataatc ccttttataa aaaattcact tgccgaaaat
     8641 tctgtcggtc tgttaaatgt atttgatgtt tctatgattt cactaatcct atccactctg
     8701 aaactacgaa tctcatttct catattacaa aaggcaacta cataccaatt attattccag
     8761 tgaacaattc catatggatt tataattcta tattttgatt ttgctttacg acctgcatga
     8821 tagtttattt ctacagatag ctcctttaat atactattct ccaatttttt aagtactgat
     8881 tctatagata ctttcccaat tggttttatt acttcaaggg ctattaagtg gtttgttaaa
     8941 atatcttctt gtttttgatt tgaatacatt tttaacttta atatcgattt atctaatgct
     9001 tcgtctaaga agtatccttc ttttttagca aatagagctg catgaagtag agatttttgc
     9061 tcttctatat caaaaaaaag aggtgcttta gtaaaattat ttaaaaggct atacccacca
     9121 ttatgaccta tatctgaaat tataggtact ccactcgcag aaagtacatc aatatatcga
     9181 tacactgttc ttatatttat ttctaatttt tcagctatct cttttgctgt aatttttttg
     9241 tcagagctta acatccacag tatagacatc atattatcaa ttttagccat gtagtttcac
     9301 ctctttctat ttgttatata aagttattat ataacttttg attgattttt tataaaaaac
     9361 attagtaaac tgctaataat ttatactaag atttatttca aatatttata gatagtcttt
     9421 ttctatagtt aagctatcca ttaaactcca tagtagtata tctacttcaa ttaatatatt
     9481 ttcaactaca cttctcgtta tatatcaatt ctctttttta aagagtttac aactgatatt
     9541 tagtttaaga tttatatata ttatttttca aaaacctttc aatctcaaat taaaacttct
     9601 ttgttttaat ttgtttaaga tatagaaaaa atcttatcct aatttattta agctaaaatt
     9661 cttatatttt aaaattttct ttaaaactaa aatatttatt atcatctcca gcaacaatat
     9721 aatctactaa ttaaatacct aatatttatt catattttca tagcttatta gttataactc
     9781 catcttcttg ctaaaattaa tgttacccaa catataattg tggaagtaat tactttagat
     9841 acatcagtta atacatctct tggatatatt agcactgagt ttatagccca tctacttaca
     9901 acttctatac ttactggttg ccactctgta tctaaacata ttactacaaa tctttctcta
     9961 tcactatcta caaaaagctt cttactaatc tataagcatt ataagaatta ctttttttct
    10021 agtttcataa agtactactt tttttgttaa tttaaaaaat ctatatctac tcttttaaaa
    10081 gatgttttct ttttattcat ttacctattt cctatatctt tctgaattga aaataataat
    10141 gtacactata tcaatgtata ttgttatata aataaatgtt cagtttcttt atttctagat
    10201 attcaattat actacattca ctagataaca aatatagtgt tgaacttgta tatatttact
    10261 tcacctttat atattctcca ctaaattttc ctaataatct ttttaatcta tttgatttaa
    10321 aatactcttt aaaaaacttt acttctttct taccttcaac tcaactatac acacatatcc
    10381 actataatct gttgattttc tgttatatcc atagttttca tcaagaacac tgacaatatt
    10441 attcactttt taagccactc cctttgaata ttattacaat aagtttaaat cttctttctt
    10501 caataaatat attatgagga agactatcat ctttaatggt ttaatagcgt ataaggatat
    10561 gaatttaaaa aatctatttt ttatatcata tgggtattgt tttttataaa actggaaaaa
    10621 cctaatagaa tctatgattt tttaacataa cctccataaa ttagtaccaa ttataacttc
    10681 aaatttaaat tttatctaaa gaatatacaa tcatatttcc agactcaaca taatattgaa
    10741 tgttgcgctt gtatttacaa attatcattc taataaaaac aattatacat agcttaaaat
    10801 catcttctaa acataattac taaaattgaa ttaatgatat caattccatt aatctctttt
    10861 aataaaaaat tctcttttct atatgaaaat tatttcaaaa ctttaaagtt aaatacaaaa
    10921 atgactataa atagattttt atttacacaa atctatctat agccactata gtttatccaa
    10981 taaaataata aaataaatat gtacacaata aatgttattt acgtcttaag tatttattta
    11041 ttagattttt aattaaaaaa aacaagctag tgaacattaa tgctaaatct agcattatat
    11101 tgctttgatc tcctgtttgt acattttgac ctgcattact atctaaactt tcatctgtat
    11161 ctatatttat atctggtttc ccatccccat ctatatctat atttacatcc gatttcccat
    11221 ctccatctat atctatattt atatccggtt tcccatctcc atctatatct atatttatat
    11281 ctggttttcc atctccatct atatctatat ttatatctgg ttttccatct ccatctatat
    11341 ctatatttat atctggtttt ccatccccat ctgtatctat atttatatct ggttttccat
    11401 ctccatctgt atctatatct atgtctggtt ttccatctcc atctatatct ggtttcccat
    11461 ccccatctgt atctatatct ggtttgctat ttcctccgtt atctgaatcc ggtttgctat
    11521 ttcctccatt acctgaatcc ggtttgctat ttcctccatt atctggattc gggttatctg
    11581 gatctggctt atttggatct ggattacctg tatcataacc tcccgagtca gtatctacat
    11641 ttttatcagg cttccaataa tcatctactc catcgttatc tgtatctatt gctggacgat
    11701 tataatctgt atcagggttt ccatctccat tggtatctat atttttatca ggtttccaaa
    11761 taccatctcg atttccacct tctgtactag gcttccattc acctgtatta tcagtatcta
    11821 tgttaatatc tggcttccaa tcaccacttg tatctatatt gatatctggc cttccatccc
    11881 catcagtgtc tatatttata tccggaatac catctccatc tatatctaca tttgtaatat
    11941 ctaactttaa cttcttttca tctttactat ctttagtgta atttatatca tctccaatat
    12001 attttacagt aatagtttgt tcagtattaa gaatatgatg attactatct aaaggtttcc
    12061 attcaataga tgcagtacta taattatgac tctcactaac cttatcatga gtttttaact
    12121 ctactggatc tttatattcc tttccattaa tatagaattg tacataacct tttggagagg
    12181 cacaatctgg tccttctttc ttatcaggtg aaacagtagc atttaatgta aacgtatctg
    12241 ttggtttaaa aacatcagtt tttactttag gcgtttctac aactaatgaa gtttctgtat
    12301 cagcaggagt aatttctgca taattataat gtgatctatg cccccaataa gactcattaa
    12361 aaggagctac actagcatac tgtttcgatg taatgattaa ttgatatttt ccaacatcaa
    12421 cttgttttgt aaggtcatca cttcctaaac ttgtatctgg ttccatcgtt tcctcattta
    12481 aacgttgaga actaataatt atatatgttc catctgtgag ttttccacct ttaggattag
    12541 ttactggaat accatctttt aatatccaat ctaaaaatgc tttttcttgc tcttttccaa
    12601 atggtaatcc atcgtatctt ttattaatcg aacctggtgg agtatttatc tcatcaataa
    12661 taaattcttc atactgcttt aaaaatggag tagttggacc tatatcttta atataaaaag
    12721 tatctgattt tagagtatct ccagtatctt tatctacaat agctaactta acagatactt
    12781 cagatccagt aaatttatca gagaaccata agtaaagaac cccttgatca tccgtatagc
    12841 taggcgctcc ataaggatac tcttttccat taatttgcat tgtaaaatca tcgactagtt
    12901 tagtattaac tttatcttgc cctaaatact ttagagatac tttgctcata aaaacttttg
    12961 ttttcttttc taagtcaccg tatgcaactg tttcaccgct acttttgaat gttgttgctt
    13021 ttaatgaacc acctgttact ataacatctc ctgttatagc acttaggtca aaaatccagt
    13081 ctcctgctcc tgtcatataa ggtaaaaccg ttcctccagt tacaagtaca cgatatcctc
    13141 tatttgctcc tggattaaca catccatctc caaatgcact accatgtgcc cctccatatg
    13201 aatagcaaag tccaccatta attgtaatat ttccactata accaccacct ttgttacgat
    13261 ctactactga tggacttaat gtatgatttc ctgatatata gtacgctccc cctattccag
    13321 ctccatggta tgcgccttta gcagttacag taccaccatt gattgtgata tctccaccat
    13381 caccgatact acctccccct attccagctc caaatccagt attataccct tccatagctg
    13441 tagcatttat aataccacca ttaatgataa tatttcctcc attttcagta tctccaccac
    13501 caatacctgc agcataagaa tttccgacag ctgttaaact ccctctgttt ttactatcta
    13561 gtagtgtaag gcgatctcct ttttttagct tgtccccatt ttctagagtt acatctctag
    13621 gaattttccc attttcagga actataaatt ctccttcaat gtctctattt acaacagaat
    13681 catcaataat tagagtagta ctttttccgg ctcttatacc tggataaagt tgatcacctg
    13741 actttagtat gtttttgcta ccatcttgta atataatatg tgcatatgca ccaccatctt
    13801 tatctggtgt taaagtaaat gggctattag atgcagtttt aatatttact ccattaaata
    13861 ttagttccgc ttttacatct ttaggtataa caatcctatg tgttgttgga gtatttggat
    13921 tattattttg aattgtcatt tgttttccac taagaatagt taaaacgcca ctttcaaatt
    13981 taaaatcaac atttatttct ccaccgctaa ttttaaagtc tcctacagaa tcatactctt
    14041 ctgcattagt atcttcttct acaagtactt cattagtatc tccttctgca tttccttcat
    14101 tagtatctcc ttctgcattt cctgcattag tatctccttc tgcatttcct acattagtat
    14161 ctccttctgc atttcctaca ttagtatctc cttctgcatt tcctgcatta gtatctcctt
    14221 ctgcatttcc tgcattagta tctccttctg catttcctgc attagtgtct ccttctgcat
    14281 ttcctgcatt agtgtctcct tctgcatttc ctgcattagt atctccttct acatttcctg
    14341 cattagtatc tccttctaca tttcctgcat tagtactttc ttctatagta tctgcgaaag
    14401 tatttgacac attaaatgat gctactgatc caagtcccat tgagatactt aaaatcattg
    14461 ataatgcttt ctttttacat ttttgttgca taaattcccc ctccccctaa taatttttaa
    14521 tatacactgt atacatccta atatatactc taataattct taatatacac tgtatacatc
    14581 ctaatataca ctctaatatt ttttaatata tacttaaaac ttatatttgt caatgaatat
    14641 actgtttttt tcaaccttat atattataag tatttattat ttgctaaata actacaataa
    14701 aaattttaaa ttatataaaa tttatttaat cttttatata ataatacatg gggtaattaa
    14761 ttgaaaaaac tagtaaaaat attaataaat attatttgca ttattacgct aattttctct
    14821 agcctttcga tttacataaa attatctgaa tataaaaagg ctgatgaagt atatactgaa
    14881 ctcagagaaa acactagcaa taacaatagt aaacatcaag aactttatga taaaaataat
    14941 gattatagat tctggcttaa aataaacaac gcaaatatag actatcctgt agtacaagga
    15001 tataacaagg atttctacct aactcatgat ttttataaaa attatcttcc tgcaggtagt
    15061 attttcatgg attatagaaa taactttgaa aatgataaga gtttaatagt ctatggtcat
    15121 tatatgaaaa ataaaactat gtttggtcag ctagaaagta gaaataaaat ataaaggaca
    15181 aacttatact tatgaaattt tttcagtata tactgattac ttaattggca gaaattattt
    15241 aagtatacac tttaataata atgatgagtt taaagattct ttgaattata taaccgaaag
    15301 atcagtacac aaatctgata taaaggtaga tatttcagat aaaattataa ctttgtatac
    15361 ttgtaactat gaatttaaag atgctcgtac tatagttcat actaaattaa tttcaaaaaa
    15421 aagactaata agtaggtttg tttaaataca gatttaccta cagtctttaa atcatataag
    15481 gatctgttta aaaatggcaa ctaaaagagc taatggcgag gggtctattg ttaaaaatat
    15541 aagaaatggt gttcagatag gatggcgcgc ttctataagt attggtcgtg atgagaagga
    15601 caaacttata agaaaacaat ttactgtaaa aacacaaaaa gagttaaaag aaaaattaga
    15661 tatttatagg acaaagatgc tcttaggctc tatagtctca gctgataaaa taacctttga
    15721 agattggttt tatacttggt tatttgatta tagagcaaaa aatttaaaac ctaaatcttt
    15781 tgaaagatat gagggcattt atagaaatca caaaaaagat actgaacttg gaaaaataaa
    15841 attaatagat ttaagagcta cacatataca aagatattac aataaactta ttgatgtata
    15901 taacaaacct gcatcaacaa ttataactct aaatacaaga ttaaagcctt gtctaacaga
    15961 aacagaaaaa caaggttata ttcaaaaaaa ttattgtaaa atgataacac tcccatctga
    16021 taacaagata aaagaggtac aaatattaag tattactgac caaaaaaaat ttattgatag
    16081 cattgatgga gataagtttg aaatactttt tcttcttgct ttgagtgctg gtttaagatt
    16141 aggagagtta cttggtttga aatggagtga tattagtttt gatgatgaat ctttaactgt
    16201 tagcatgact ttgcaacgag ttactgaaat aaataaagat ggaactagag aaaaaaagtc
    16261 attgaacagt atacaaagac taaaaattct ataagaacag ttcctattcc aagaaatgtt
    16321 ttagttaaat taaaaagcat aaaatacaac aaacagaaca aagattattg ttaggtgatg
    16381 cttatataaa taataattat gtaatttgta atgatacagg tcttgcttta aacaataata
    16441 gacctggtaa aattttagat tctttactaa aaaaattaaa tatacctaaa attaaatttc
    16501 atgctcttag acatacatat gcaacacgat tattcgaggc tggtgtccct cctaagactg
    16561 ttcagacatt aatgtgtcat tatgactatg gatatataca cacgttatgc acaataccaa
    16621 gcaggatgct gtggataaaa taaatgatat tttttaatgt aaatgaagtg aaattaaatt
    16681 tattaacagt aattaaaatg ctataatatg tatattatat ttagattaag aaaagtcata
    16741 tatttaaata catggctttt cttaatatta tttgtctaca atatgacagt ccttttattt
    16801 tgatgaatct agtatctcac caaataattt tctatactct tctgataaag atacatttac
    16861 aagttcacac catatatgct cttgaatatc tatattttca atacaccata ctttataatg
    16921 gcactcctta gttatataaa aatcaatttt aatttcattg gtagaattat cttcttgctt
    16981 ggaacagtat tctttaaatg gatactggtt ttttataaaa tctatatgtc taaatgcttt
    17041 tcttttagga taatatttac catgaataaa agttgttata atatctttat ttgatttaaa
    17101 ccctctgtct atatatggaa gttcttttat ttcaatatgc caaaactctt catcaagaat
    17161 atcatcatat ctctttttta taaccataag tagtttatta taattatcat tatctccatg
    17221 aacaataata tttttcaact tttctagctt agtattagaa aaatcaaatg tacatcctcg
    17281 ccatttttgg aattttaaaa tagtactaac atatgcatac ttcaatggta ctgcaagacg
    17341 ttcatctaat cttaaataaa aatttgattt attaaaatct atattttggg acataatttc
    17401 aaaaactgca ggtataagac tatttatatt tatttaaagg agatttatca ataaaaatgt
    17461 tatataagta atatttttta tcatatataa aaccattttg tttaaagtcc aagttttcta
    17521 ttttagtaag tttataatca tttatatcat acatatgttt ttcatatgaa attactgact
    17581 taatatttaa atcatcatat ctaaatctat ccaacacaaa acatgtaaca acacaaaaca
    17641 atagatcttc atcaatggta atattagttg gaaattcttt ttccccacat gaaatactat
    17701 atatatctga atatgccaca tccctaggaa atgtatagga aagtatttac tgtgctacaa
    17761 aaaaataatg tgttcttttc ttggggtcag taatatattg aaatatttcc gattgagtta
    17821 aatttctttc tttaattaga gatttaactt cattacaaag ttgtttaata taataaggaa
    17881 caccactcca attcctcacc ttaagagatt tccataaagg ctcatctaaa tgaaaagtat
    17941 aataacttgt attatagtaa ctatctaaat ttttgatatc ttgtatcaat ttttccatta
    18001 ttgttatccc cctaaaaatt ttagtctaat tgtattgatt tacataagct tctagttgtg
    18061 cgctaattgt gtgtctttca ataatgcaaa agggattact cagtctaaat ccaagtaatc
    18121 ccaatcattt catcattctt ttttcaaaat tggtggagac gaatcgtcag aataataccc
    18181 acgattaatt ttgttattat atattaatac tactactcat taactaaaat ttgaattaaa
    18241 tcaaatacta atttgaaatt ttcaaaagat atattcttga actcctctac tccatttaat
    18301 atactatctg caaaatcatt cttgcttagt gcaatattat cctcaggatt cttaatatca
    18361 catactacac accccgaatc tataatagac ttctttccac atttattttt atcttgacat
    18421 attttatccc ctaatatatg taatcctagt ttagaattaa attcatcgcc taaaaataat
    18481 cttctacctt tgtagaatgt ttttatttct tcatcagaat aataatgttc tatacacatt
    18541 attttatcaa tttcaaccct gttatccggc aatggcaata ccattgatat tacattatta
    18601 ccccataatt tataatttgt attttcatct tttacttttc ttagatccct atcactcaag
    18661 tctctatcaa aaacacatat taccttatta ttgttcctta atttacttgc cgcttcacaa
    18721 atatttatta aattattata gcccattttt atatcatttt catactctaa aaattcaata
    18781 tctaaatcat gatataatcc ttgctctttt aattttaata aaccagcttt catatgtttc
    18841 caatctgtct ttccttctgt aattattaat gtctttccat ttttactttt taatatatct
    18901 tgtatatctt ttgaaaataa ttcagtcctc ttataatatt catatgatct ttcaaattcc
    18961 gaaaactctt ccggtccaat gttctctcct gttggcattt catatatctt aaatccatcc
    19021 tctttaaata tatctttcat acctaatata aataatggag aatgagatgt tattataaat
    19081 tgaacctttg gaaacttttt tattagtttt ggtagtacgt cgcattgtat actcgaatgt
    19141 agatgtaaat caatttcatc tattataaca ataccttcta tttcatttaa ttttatactc
    19201 ttattaatat ctccataatc tgaatatcta attattgttg aaaacatgtt aaataatgct
    19261 gactctcctg tagataaagc atctaatgta ggtataatca ttgaattggt cgacctatca
    19321 attatattaa atctggatcc acctgaattt ctccaattta atccaaactt aacatccttc
    19381 tttactattt cagataatat ttcttccaca ttttctcttg ctatttttag taatcttata
    19441 ttatttaagt cttgttccga attccatcca cctaggtcac tctgtattaa atcagctcta
    19501 gaatctacaa ttatatctaa aagccattta atattagttt cagttacatt ttgtacgatt
    19561 atgggtttat ttaatttacc agatatttta tcatttattg ttataagatt ttctgagatg
    19621 atattttgat tcatccagtg aggtgtttca tatctattcg gaggaaaaaa acaaaaagat
    19681 ttatttgcaa attcttcctc aaaatactct ttatttttag ttatatcttt tatatttttt
    19741 ctattacccc agttattatc taaatttaat agtccactag tcttttctat gcattgctca
    19801 aatgataagt tccctaattt ttctatatat tgaaaatttt gttctccatt tacaaagttg
    19861 ataaatgaaa aagaataatc acatccaacc tttgtgtttt tccaagataa aactttaaaa
    19921 taaggtgttg taccatcttt atttgtttct gttatatcat tgtacggctg ttttgcaaat
    19981 tcaaataatg aatcaactat gttgcttaat acagttgatt taccctttcc attttcacct
    20041 acaaatataa ttggttttgg acttccatct tcattgaatg gaaaccctat atctaagtta
    20101 tctataggtc ctacattctc cattattatt ctgtctaaaa acatttacta tcttcccctt
    20161 tatatattta tattaaaaaa gtattgatat ttatatcaat acttttcgtg gatttcaatg
    20221 gtggagacga ggggagtcga acccctgtcc gcaaatcttt tccttgctgc atctacatgc
    20281 ttagccaact tcactaatat ctcacctcac atagcgccaa gtggcaggct ctatgcttag
    20341 ccagtcccaa ttaatctact ttaagtcgag acacccctaa agtagttccc tgcataatga
    20401 cgtccagtct tagtcggcag gaggactaag agggacgagc agcaactagg ctgctatcgc
    20461 gaaattatcg tctgcgttta ttttaaagtt ccccagtttt tacgtgaccc agagtaacac
    20521 gacatgcagc ctcaagttcc aaacccacgt cgaaaccttt acgccccctt gagtaattta
    20581 tagtccagta gactcttcgc aaactttatc accattttta ttaaattttt aatgtatata
    20641 taatataata cttttaagaa taaaaatcaa tgttatgttc tagataataa agaattttat
    20701 tctaaaaaat acagttttat ctagtttaat acttccccgt catttctctt tctatacgtc
    20761 tttgtgcatc tttcttagcc atatcttgac gtttatcata tagtttttta ccttttgcta
    20821 atgcaatctc tactttaacc ttaccatttt ttagatacaa actcagtggt ataagtgaat
    20881 acccctttat agtagttgct ccaataagtt ttcttatttc atgcttatga agcaataatt
    20941 ttcttactct taaagaatca acattgaata tatttccttg ctcatatgga cttatatgaa
    21001 cttgttttaa aaatacttca ctattatcta cagatgcaaa tccatcactt aagttaactt
    21061 taccttgtct tattgatttt acttctgtcc cttttaattc gattccacat tcataggttt
    21121 cttctataaa atattcatgt cttgctcttt tgtttgttgc taatattttt ttaccagcca
    21181 tataaatctc accttctttg ctagaaattt cttcaataaa tattagaaat ttgctaaata
    21241 tttattgtca ccaaattatt tgactaactt gtatgataac atatttaggc attttagtca
    21301 atatttcact gatatattaa gttatttagg ttatatataa taaacaatat aaatatataa
    21361 aaataattaa aaaactattt gaaatatact taatattaaa ctacatttaa ccagtaacac
    21421 tactttataa acttcaaaac aaaaatatta catattataa attttctat
//
LOCUS       DAFNNB010000055        21401 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0055, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000055 DAFNNB010000000
VERSION     DAFNNB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0055"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            146..2380
                     /locus_tag="L3M60_001778"
     CDS             146..2380
                     /locus_tag="L3M60_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6226448.1"
                     /translation="MQNHDYIKMLNKKVKLLSKELDNRNFKLKSSNEIDFFSKMPNRE
                     FMYQYIDDCISKNVHIGIMLLNIDNFKLFNDIYGYLVGENLLITLTQKLLHFIPESKG
                     IVAYLKGEGFLIVLPNVSNKELSTMGTTIVESARELKIQLPNEKEIYSNITFSIGGAI
                     WNGESNMTTLMLFNQVYSALKKAKSEGKNRYTLFDFKDNTFYNKLENDKEFLSEISYA
                     LQTKQIEVFYQPLYNIKNNTIVGCEALARWRHPIKGLLTPDKFIPLFEQFGLITELDL
                     YLFEENCRNIRKWIDNNDVFVPICCNFSRLHFFNPNFPSMLKGITEKYSVSTSNFSIE
                     ITENILIENTNTIIQQLTELRSYGFSVSMDDFGSGYSSFGMLQNLPFDIIKIDRIFLS
                     HNLNDFKNTTILYSIINIARALGMLTVCEGVETEQHVEFIKSINCDIAQGYYYAKPME
                     CKQFEKLLSKGNNSEKMFIKYSDIGKALIEKIFDGFFIMQDMKKLEANVTEDIQWFDF
                     YNKTEITSFPKVIEHFEKHIKGKKFSILYKSITPHNTESDSLLISGEAVLSDEAAEHQ
                     KYSNFYFSANCIISENKLLLSKLHLDLINTAGYINEFESYSIPKMANTHLDESDILSQ
                     FYSMLPVGIIRYDLFGDMIITYMNEEMLDIIGYTKEQFFGEMGGNLRAIVHPDDLDFV
                     YKKSLEMIDTNKIEPFLYRFIKRDGQIATVMYTQCNLSAIDGRSIAQGMYVDINKIKN
                     LTKI"
     gene            2411..3256
                     /locus_tag="L3M60_001779"
     CDS             2411..3256
                     /locus_tag="L3M60_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6226449.1"
                     /translation="MFKEIFLRTFPGFLIIRDSNYRITFINDNLKNLIKSYMQDNPLG
                     MTNVEIAKKLPDNIAKFFTDYHNIQLNWEKDYPYDKISNWILEFKKDTTSYWNVLEYK
                     VDVDEKSYIITMANDITKLYEENKKNLHYSITDPLTGAYNRKYLNDRFDMFIGDYIVL
                     IDLDNFKMVNDYEGHNVGDKILCDFVSLLNKELINSTSIIRLGGDEFIVIFSSDVDKN
                     YVYSQIEALRENFLKVFSKYKYLSFSYGVDTVKRNLKLTIVELDKKMYKNKEKKKKKF
                     NKNNY"
     gene            3496..5229
                     /locus_tag="L3M60_001780"
     CDS             3496..5229
                     /locus_tag="L3M60_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6226450.1"
                     /translation="MSILLKKAPKLAKHIITSFYINRDIDEVLKYLCENVTWIGPGEQ
                     EFLTSFNEIKNYFYAGQYEIPSCDINNDIFEIVSEYENRCMVLGKYTVRTKENAQMIL
                     EVNQRCTFEIIEDREKLLVKHMHISNPYGEMQLDEYFPTKIGTQSYDYLQRLLKEKTE
                     VIEMITNNINGGLKGSNDDSTYSFFYVNEGLPKILGYTYNEFMEMSGGSAVGAVYPPD
                     LPKALEDCEQCFAKGPTYSSEYRIRKKDGTLMWVLDSGMKSLNSDGIVKINSIITDIT
                     QLKNIESELKLERERYRIALQNITDIMFEYDMENDNFIKYQRVEIDKKIELENFETKN
                     YSKLLESGKIIHLDDIGKLLEVLRGNLHETIEIREINSLTKNEWRWIRVQCSVIYDSD
                     HNPIKTIGVLKDITEDKSKLESLINQAQRDPLTQLYNQRVSQNLIQEYLCSSDSKNND
                     ALLIIDIDDFKTVNDTFGHLEGNEVLVAVSKILLHNTYDKDIVARIGGDEFTIFIKSL
                     TKDLIIKITNDILNDASKIKVKDNHKITLSIGIAFTDDSTKLYKDLFSKADKALYLSK
                     ADGKNCYSVYE"
     gene            5696..6040
                     /locus_tag="L3M60_001781"
     CDS             5696..6040
                     /locus_tag="L3M60_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6226451.1"
                     /translation="MKNELYLICLNKRFNLWVQPTFVAPFKELLIDNNLRDIKLHKLR
                     HANATLILLSSTNIKSISEILGHADIKITMNRCSHLLYEMDNEASENTIQIMFSFTSI
                     KCQLKHFFLLIS"
     gene            6231..7586
                     /locus_tag="L3M60_001782"
     CDS             6231..7586
                     /locus_tag="L3M60_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6226452.1"
                     /translation="MDTKISINENMSLGKRFFKYLAPSVVAMWVFSLYTMVDGIFVSK
                     GVGELALAAVNISMPFINFIFAVSLLFSTGASTIIAIYLGKKDIKSANEVFSFNLVSI
                     IILSIIILAITFFNLDRLALFLGATESTIGMVKDYLGIIIFFNGFFIVSYSLEVIIKT
                     DGFPILATVGVIISAITNIILDYLFVIEFGWGVKGAGIATGLSQVFSTIFFLIHFLRK
                     NSTLNFSKFRIDFKTLRKIIFIGFPDSTTELSCGIVVLLFNLSLTKYIGENALIYYSV
                     INYINTLVLMTMMGITQGMQPLTSFYYGAGNIDNVKKLLKMGIKATVIASISVFAVCI
                     AFSEPIVSLFIHPEETILFNEGVRVFKIFSISFLLVGINVIISGFFVSVEKPSISTVI
                     SLGRGLVIVVLSLISMILIFGGQGIWMTTIVSEFICLILSLVFLKKNFSTLDSNLNKV
                     A"
     gene            complement(7704..8372)
                     /locus_tag="L3M60_001783"
     CDS             complement(7704..8372)
                     /locus_tag="L3M60_001783"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HBN6226453.1"
                     /translation="MKEVLKLADEKFVMSFSGGKDSILALNRMLKKGYKPVALLTTIS
                     EEHGKSWTHNLEYNMLKQVSSNIGLPLLVAECGVEGYEESFERALIKAKNMGATICAY
                     GDIDIESHRKWDTDRCEAVGMKVDLPLWQESREDLVYEFIDSGFCSVVTKVNLKHLGE
                     EFLGKKLTRELVEKIKDAGADPCGEHGEYHTFVVDGPIFKKPVEYEVKGTLVKDGYGY
                     LEIK"
     gene            complement(8439..8867)
                     /locus_tag="L3M60_001784"
     CDS             complement(8439..8867)
                     /locus_tag="L3M60_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN6226454.1"
                     /translation="MTKLWAIIFALLAGGSTALEAFINGELGKSTTALVATFISLTVG
                     AIFFLASIIVTGDLKCLVSINGFNYKLLLGGIFGGLIIYFTVKAIPNLGVSNTLILTL
                     VSQVLVGFFIDSVILGQETMHLYKYIGVILLILGTFFIVN"
     gene            8979..9572
                     /locus_tag="L3M60_001785"
     CDS             8979..9572
                     /locus_tag="L3M60_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HBN6226455.1"
                     /translation="MNFCYNCSIDFGGDFMYTNFNIDFNNFNKKENATKFMLMGVLLI
                     ILGLLCLTFKTLGIKLISWTFGIALLFFAYLNLKNINELKRYATKEEIKPSINIQWIL
                     IIACILLFVFPQKIQSIFSLLLGFYLIFNQLVALVNSKNNPYSKFTTWNIVKILFGIC
                     LILSPLFLSRFIVSIMSFFIILFGLVLFFSGNTARKY"
     gene            9692..10627
                     /locus_tag="L3M60_001786"
     CDS             9692..10627
                     /locus_tag="L3M60_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893027.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBN6226456.1"
                     /translation="MNKFKYAFDNKRYHTWNYYLRNTFGEKVFKVSINAGFTCPNIDG
                     SIGYGGCTYCSKEGSGDFAGNPKDNLISQFYNIKEMMLKKWPHAKYIGYFQAYTNTYA
                     PLEVLKEKYETILELEDVVGLSISTRPDCLPDDVVEYLSELNKRTNLWVELGLQTIHD
                     STSKIINRGHDYKTFVEGVEKLKSKNIKVVVHIINGLPGEDYNMMMETAKAVGKMDVD
                     GIKIHLLHVIKDTPMEKMLQNGMLTLMEQDEYINLVCDQLEILPETMIIHRLTGDGKR
                     DELVGPIWSLKKWEILNQIDDTLKSRNSYQGCKFV"
     gene            complement(10929..11873)
                     /locus_tag="L3M60_001787"
     CDS             complement(10929..11873)
                     /locus_tag="L3M60_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBN6226457.1"
                     /translation="MGEKSKGYVFIAIAGLLWATLGLFGKFLMGNGLASEQVAFTRLF
                     FGFIVLGIYSSIRTPQILKISKKGIIYSVIIGIICQAMFNLCYFKAIDIVGVSIAAVL
                     LYTSPLFLAIFSKVCYKENITRSKLFSLILCFIGAIMAVTGGRLDFQGLNAFGLLLGI
                     LSAIAYALMPTISKNALKEFSSSTILVYSFLFGAIFMIPSSKPWEILNYANNLDVLFC
                     MLMLGIVPAALAYIFYATGISKGVELSVAGVVASVELVGSVIIGCTILGESFSLGKLF
                     GVMLMLISAVVALNLSYDEIRIFYKSSKLKQIEKTESI"
     gene            12191..13633
                     /locus_tag="L3M60_001788"
     CDS             12191..13633
                     /locus_tag="L3M60_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6226458.1"
                     /translation="MNLNLELDLEFYKKILEASHDEICVSDDKGIIIYCNKAFEENYG
                     LKKEDILGKNVSFLEDSGYSTKSPIPVVLKTKSKFSLEQDTQTGKKLIITATPIFDEN
                     GNLEFTVENCRDITELNNIKNKLEDTKKQVKKYKSEVETLYRTALRIEDTVIMDGIVM
                     RPIINTVNHVSKTDVSVLLLGESGTGKSSLARYIHHNSNRANGPFITINCATISPQLL
                     ESELFGYTSGAFTGASTKGKVGLVELANGGTLFLDEIGDIPQNLQAKFLQLIQDRTFT
                     PVGSLKNKNVDIRIISATNADLVSKVKEKKFREDLYYRLNVIEIKLPPLRERRDNLVE
                     IIKYYFNRYSSDFNLNKTISKEAMETIANYRFPGNIRELQNIIQKILLTCTDNHITIH
                     NLPNILTKNINITNNGNKTHISQINKVITPDSKSINYKNKNFDTLIKEYEKNIILDAY
                     EKFGSSYKVAKHLEISQSKANRLIRKYTNT"
     gene            13833..14417
                     /locus_tag="L3M60_001789"
     CDS             13833..14417
                     /locus_tag="L3M60_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HBN6226459.1"
                     /translation="MLMNTHFIMAKSILDNIDENKTFFISEKNFIYGNIKPDAFSKYK
                     LKKHYLDESFNMIVSKINYLCSLNIDSLSKYFSVSRLSQELGVICHFLCDFFCVAHSE
                     RWEFKHSLNKHVTYERELGAFAKDVDLSKIKCGTINSSFEKFFTKLYSDYKKKTDYMN
                     DLKFSAYTCNSVINYILDSILSNTIKSYHIANCG"
     gene            complement(14511..15290)
                     /locus_tag="L3M60_001790"
     CDS             complement(14511..15290)
                     /locus_tag="L3M60_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBN6226460.1"
                     /translation="MIKHIFCDLDGTLYENGTITKEDMVAIEEIEKKGVQFNVATGRI
                     FKQARNIIEESLDMNGYYICENGSFIYDKDYNVIFKRTIDDNLVKKVIDRFESSDAQL
                     YFKYKGDVIVGSDTTAFRHYSSDFIVDPDFEKRSSFDNLIGNIGVCSENLEELSRIEL
                     YLKSEFSEVLEIYFSGTYTLNIVPKSVSKRGSIEHVIKTLNVSPDEVATIGDSPNDIC
                     MLEGFKYSFAMSKAREDVKQSANYVVDSVKSAIDVIMEINS"
     gene            complement(15348..16604)
                     /locus_tag="L3M60_001791"
     CDS             complement(15348..16604)
                     /locus_tag="L3M60_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011591286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HBN6226461.1"
                     /translation="MKAEIISVGTEILLGDIVNTNSQFLAKELASLGIEVYHQSTVGD
                     NKQRLLECFAESLKRSDFVITTGGLGPTGDDMTKETAAEYFGQKLELHKPSLEVLESF
                     FVKTGKKMAENNMKQVYFPKDAIVLKNNNGTAPGAILRKDGKFIIVLPGPPREMKAMF
                     NESVKPYLQQFTNEMLVSKTLRLYGIGESNLELEILDIINEQTNPTVALYAKELEVTI
                     RITAKAENEGEAFKLIKPVEEKIKSRVGKYVYTEGDISVSEGETALEDAVSKLLVEKN
                     LTIAVAESCTGGLVSSSLINYPGISSVFLEGCVAYSNDSKMKRLGVKRETLEEFGAVS
                     EQTAIEMAEGVAKGLKANIGISTTGVAGPGGGTKEKPVGLVYTAIYINGKTIVRKNIF
                     NGDRRKIRLRATRDLLNELRIQLEKL"
     gene            16862..17761
                     /locus_tag="L3M60_001792"
     CDS             16862..17761
                     /locus_tag="L3M60_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBN6226462.1"
                     /translation="MKIAVIMGGISSEREVSLNSGKEIYNNLDKNKYEVVKVIIDDKK
                     DIFTKIPEDIDFAILALHGKFGEDGCIQSILETMDIPYSGCGPLCSGMCMDKNITKKM
                     LRDSNLPTAPWVLVKSVDEIDYDEIDNIGYPVFIKPNSGGSSVATFFIHSKDEVGEAV
                     RKGLEVDEFVMIEKYIPGGEYTSFILNGEVFPTISIKSDSGFFDYEAKYSVEKGAKEE
                     VVYLDEELQKRVNEISETCWKIFNCKAYVRVDMIISEGIPYVLELNTLPGMTQTSLIP
                     RSAAARGIKYSELLDKLIEYSLN"
     gene            17785..19233
                     /gene="ddlR"
                     /locus_tag="L3M60_001793"
     CDS             17785..19233
                     /gene="ddlR"
                     /locus_tag="L3M60_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DdlR"
                     /protein_id="HBN6226463.1"
                     /translation="MIFSNLKLNDNEPIYIQIKNYISDMISKGLIPDNSKLPSTRELS
                     QLLQVSRNSVVLTYEELKSDGLIYSISGKGTFVKSKNKSSNTTWSLNWDYLENTYSKK
                     ASELDIIKSEIPWSSDLISFKSISPDGDLFDMEELKKSFLNRISLEGHKLLNYGYAQG
                     YKPLIDYLLKYMTNKGVDISNKDILITNGFTEGFDIVMSSFTQEKDYIICENPTHNTA
                     IKIMKSHNLNILGVDINEDGLDFDMLEYNLIKYKNKIKFAYITPSYHNPTGIVMTPEN
                     RYKFYNLMKKYNIAIIEDGFNEELLYNSSHIFPICSLDNMNNGVVYIGSFSKILFPGM
                     RIGWIFADKKVINKLESVKRCRNIHVSFLDQGILFDYLSNGGFEKYIKKIRKFYGDKF
                     NFAYECVKKHIKTEYILGDGGLHIFIKLKNIDTRVLLEKCYEKNVIFMPGDLFFTDNS
                     GFDTLRLGFSRLSFEDIEIGIKIIGETINEMF"
     gene            19292..20098
                     /locus_tag="L3M60_001794"
     CDS             19292..20098
                     /locus_tag="L3M60_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBN6226464.1"
                     /translation="MNLILGDNMSKKSKRKKINKLKLTIVITLLFLALLSIYEILFSN
                     PNKKDNVKSQETMQNKNNTTDDSKNNQSSTKKQEDNNIKPTFSYSSIPDNIKNKMIGK
                     SMPTDEPISFDSLSYLKLTYYGFDEKTHQGEMIVNSELAPEVVDIFKELYEKKYPIEK
                     INLIDDYDAVDEKSMSDNNTSSFCYRTIAGTNVISNHGKGRAIDINPLQNPQVSGNDV
                     TPKVSTVYADRSSTKFGMIRKGDDCYNAFVSRGWSWGGYWKNPDYQHFEK"
     gene            20124..21308
                     /locus_tag="L3M60_001795"
                     /pseudo
     CDS             20124..21308
                     /locus_tag="L3M60_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
BASE COUNT         8144 a         2850 c         2899 g         7508 t
ORIGIN      
        1 acatttgaac aaataactta atgtatatta attttaaata cgtatttaaa attaattgtt
       61 taaattattt atagattaaa atttaaatac ttctagcata aatacagttg tcgtcaaaat
      121 ttaaagtatc ttatggagga ataccatgca aaatcatgat tatataaaaa tgttaaataa
      181 aaaggtaaaa ctattatcta aggaattaga taatagaaat tttaaattaa aatcctcaaa
      241 tgaaatagat tttttttcta aaatgccaaa tagagaattt atgtatcaat atattgatga
      301 ctgtatttcg aaaaatgtac atataggaat catgttgtta aatattgata attttaaatt
      361 atttaatgat atatatggat atctagtggg agaaaattta ttaattactt taactcaaaa
      421 actattacac tttattccag aatcaaaagg gatagttgct tatttaaaag gagaagggtt
      481 tttaattgta cttccaaatg tatcaaataa agaattaagt actatgggta caactattgt
      541 agagagtgct agagaattga aaatacagct tcctaatgaa aaagaaattt actctaatat
      601 tacatttagc attggtggtg ctatctggaa tggtgaatca aacatgacta ctttaatgct
      661 tttcaatcaa gtatatagtg ctttaaaaaa ggctaaaagt gaaggtaaaa atagatatac
      721 tctatttgat ttcaaggata atacctttta caataaattg gagaatgata aagaattttt
      781 atccgaaata tcatatgctt tacaaactaa acaaattgaa gtattttacc aaccacttta
      841 taatataaaa aataatacta tagttggatg tgaggctctc gcacgatgga gacaccctat
      901 taaaggactt ctcactccag ataagtttat tcctttattt gaacagtttg gtttgataac
      961 agaactagat ttatatttat ttgaagagaa ctgtagaaat ataagaaaat ggattgataa
     1021 caatgatgtt tttgttccaa tttgttgtaa cttttctcgt cttcattttt ttaatcctaa
     1081 ttttccaagt atgctaaaag gtattactga aaagtacagc gtctctacat caaatttcag
     1141 cattgaaata acagaaaata tattaataga aaatacaaat acaattattc aacaactaac
     1201 tgaacttcgt tcctatggtt tttctgtatc aatggacgat tttggttctg gatattcatc
     1261 ttttggtatg ttgcaaaatc tgccttttga tataatcaaa attgatagaa tatttttatc
     1321 tcataattta aatgatttta aaaatacaac cattttgtat tctataataa acattgcaag
     1381 agctcttggg atgcttacag tatgtgaagg tgttgaaaca gaacaacatg tagaatttat
     1441 taaaagtata aattgtgata tagctcaagg ttattattat gcaaaaccta tggagtgcaa
     1501 acaatttgaa aagttactat ctaaaggtaa taattcagag aaaatgttta taaaatattc
     1561 tgatatagga aaagctttaa tagagaagat atttgatgga ttttttatta tgcaagatat
     1621 gaaaaaacta gaagcaaatg ttactgaaga cattcaatgg tttgattttt acaacaaaac
     1681 tgaaattaca tcttttccta aagtaataga acattttgaa aaacacatta aaggtaaaaa
     1741 attttctatc ctttacaaaa gtataacccc acataatact gaatcagatt cattattgat
     1801 tagtggagaa gctgttttat cagacgaagc tgctgaacat caaaaatata gtaacttcta
     1861 ctttagtgca aactgtataa tttcagaaaa taaattactc ttatcaaaat tacatctgga
     1921 tttaattaat actgctggct atataaatga atttgaatca tattcaatac ctaaaatggc
     1981 aaatacacat cttgatgaaa gtgatatttt atcacaattt tattcaatgt tgcctgtagg
     2041 tataattcga tacgatttat ttggtgatat gattattact tatatgaatg aagaaatgct
     2101 tgatattata ggttatacaa aagaacagtt ttttggtgag atgggcggaa atttaagagc
     2161 aattgttcat ccagatgatt tagactttgt ttataaaaaa agtttggaaa tgattgacac
     2221 aaataaaatt gaaccattct tatatagatt cataaaacga gatggtcaaa ttgccacagt
     2281 tatgtataca caatgtaacc tttctgcaat tgatggaagg tcgattgctc aaggtatgta
     2341 tgtagatata aataaaataa aaaatttaac aaaaatctag tacaaagtat tttattttgg
     2401 agaaattaat atgtttaaag aaattttttt aagaactttt ccaggatttc taattataag
     2461 agattctaac tatagaatta catttataaa tgataattta aaaaatttaa ttaaaagtta
     2521 tatgcaagat aatccacttg gaatgactaa tgtagaaata gctaaaaaac tccctgataa
     2581 tattgccaaa ttttttacgg attatcacaa tattcaatta aattgggaaa aagattatcc
     2641 ttatgataaa atatcaaatt ggattttaga atttaaaaaa gatactacaa gttattggaa
     2701 tgttttagaa tacaaagtag atgttgatga aaaatcctac ataattacta tggccaatga
     2761 tataacaaaa ctgtatgaag aaaataagaa aaatcttcac tactcaatta ctgacccatt
     2821 aactggagca tataatagaa aatatttaaa tgatagattt gatatgttta taggagatta
     2881 tattgtacta attgacttag ataattttaa aatggtaaat gattatgaag gtcataatgt
     2941 aggagacaag attttatgtg attttgtttc acttttaaat aaagaattga taaattctac
     3001 ttctattata agattaggag gagatgaatt tattgttata ttctctagtg atgttgacaa
     3061 aaattatgtt tattcacaga tagaagcctt aagagaaaat tttttaaaag tattctctaa
     3121 gtataagtat ctttcattta gttatggtgt tgatacagta aaaagaaatt taaaattgac
     3181 aatagttgaa ttggataaaa aaatgtataa aaacaaggaa aaaaagaaga aaaaatttaa
     3241 taaaaataat tattagcatt gtcattttaa aataaattaa aattagcttt cttttttaaa
     3301 acttttacct tagcttataa atatgtatct atacaaggta ttaatattaa atactgaaac
     3361 atatattgtt tcagttcttt tttgttaaaa ttaacttatt caataaagat atcatagttc
     3421 aacaaagctt ttaatttaaa tatattgatg tataatataa ttaaaatata atagcaaaaa
     3481 gaaaggagga atatcatgag catactccta aaaaaagcac ccaaattagc aaaacatatt
     3541 ataacaagtt tttatataaa tcgagatatt gatgaagtat taaaatacct ttgtgaaaat
     3601 gttacatgga ttggaccagg agaacaggaa tttctcactt cttttaatga aataaaaaat
     3661 tatttttatg caggtcaata tgaaattcct tcttgcgata taaataatga tatatttgaa
     3721 attgtaagcg aatatgaaaa tagatgtatg gtattaggaa aatatactgt tcgaactaaa
     3781 gaaaatgctc aaatgatttt agaggtaaat caacgttgta catttgagat aattgaggat
     3841 agagaaaaat tattagtaaa acacatgcac atatcaaacc cttatggtga aatgcaactt
     3901 gatgaatact ttcccactaa aattggaact caaagttatg attatttgca aagattacta
     3961 aaggaaaaaa cagaagttat tgaaatgatt acaaataata ttaatggtgg cttaaaaggt
     4021 agcaatgatg atagtacata ctcatttttc tatgtcaatg aaggattgcc caagatatta
     4081 ggatacacgt ataacgagtt catggaaatg agtggtggtt cagcggtagg tgctgtatat
     4141 ccaccagact tacctaaagc actagaagat tgcgaacaat gttttgcaaa aggacctaca
     4201 tattcatctg aatatagaat acgaaaaaaa gatggaacat taatgtgggt attagattct
     4261 ggaatgaaat cattaaatag tgatggcata gtaaaaatta atagtattat tactgacata
     4321 acccaactaa aaaacataga atctgaacta aaattagaaa gagaacgata tcgtattgct
     4381 ttgcaaaata ttacagatat catgtttgag tatgacatgg aaaacgataa ctttataaaa
     4441 tatcaacgtg tagaaattga caaaaaaatt gaattggaaa attttgaaac aaaaaattac
     4501 tctaagctat tagaatctgg taaaattatt caccttgatg atattggaaa actactagaa
     4561 gtacttcgtg gaaatcttca tgaaacaatt gagataagag aaataaattc attaactaaa
     4621 aatgaatgga gatggattcg tgttcaatgt tcagttatat atgatagtga ccataacccc
     4681 atcaaaacaa taggcgtttt aaaagacata actgaagata aatctaaatt agaaagctta
     4741 attaatcagg ctcaacgcga cccactaaca caattgtata atcaaagagt atcacaaaat
     4801 cttattcaag agtatctgtg tagttctgat tccaaaaata atgatgctct attaattatt
     4861 gatattgatg attttaaaac tgttaatgat acttttggtc atttagaggg aaatgaagtt
     4921 ctagttgctg tatcaaaaat actattacat aacacatatg ataaagacat agttgctcgc
     4981 atcggtggag atgagtttac aatatttata aaatcattaa caaaggattt aataataaaa
     5041 attactaatg atattttaaa tgatgcaagt aaaataaaag taaaggacaa tcataaaata
     5101 acattaagca ttggtattgc ttttactgat gattcaacta aattatataa agatttattt
     5161 tctaaagctg ataaagctct ttatttatca aaagcagatg ggaaaaattg ttatagtgtg
     5221 tacgaataaa gccatttata tggctaaaaa tgaaccttta aattcaaatt catagtctaa
     5281 caatcaaaga gtcaaagcta atcttataga tagccaacta gttactagaa aaaatttagg
     5341 cactatattt attgatactg ctcatttttc caaagagcaa atttatataa aaaattaaac
     5401 ttttatgtat gcacaaatac taatgataaa cattttgtta caaacattta tcattagtat
     5461 gttctatctt aaaacaaaat gataatttat ctatataaaa aagtgctttg ttgacagagt
     5521 tcactttttt atatagatag tttaaatatg aatacatgtt ttatcccata cattttggtt
     5581 aatataaaac cttgtatctt attatattca ttcatctgtt cttaaaattt acataccaca
     5641 tagctaatat aaaactaaaa tcaccccttt aattaactca ctttctacat gtatattgaa
     5701 aaatgaatta tatttaatat gcttaaataa aagattcaac ctatgggttc aaccaacttt
     5761 tgttgctcct tttaaggaac tattaataga taacaatctt agggatataa aattacataa
     5821 attaagacat gcaaatgcaa ctttaatatt actttcaagt actaacataa aatctatttc
     5881 tgaaatatta ggacatgcag atataaaaat aactatgaat agatgttctc atttattata
     5941 tgaaatggat aatgaagcat ctgaaaatac tattcaaata atgttcagtt ttacatcaat
     6001 taaatgtcag ttaaagcact tttttctact tatcagttaa atgtcagtaa tatatatagt
     6061 aagatatact tataaaacgt ttgaaatcac tattccttaa taaatgttat aattatttac
     6121 tatttgctgt tgacattggt attataccat agtttatcat ttatattaga aattaaatta
     6181 ttaattaata gtttcgtaat tttacaacag atttacaggg agtgataata atggatacaa
     6241 aaatatctat aaacgaaaac atgagtctag gtaaaagatt ttttaaatac ctagcaccat
     6301 ctgtagttgc aatgtgggtt ttctcactat atacaatggt tgatggaata tttgtaagta
     6361 aaggagttgg agaattagct ttagctgctg tcaatatatc aatgcctttt attaatttta
     6421 tatttgcagt ttctctatta ttttcaactg gagcatctac tataattgca atttatcttg
     6481 ggaaaaaaga tattaagtct gctaacgaag tcttttcatt taacttagta tcaataatta
     6541 tattatcaat aattatatta gctataactt tttttaattt agacagatta gcactatttt
     6601 taggtgctac agaatctaca attggtatgg ttaaagatta tttaggaata ataatatttt
     6661 ttaacggatt ctttatagtg tcttattctt tagaagttat aataaaaact gatggctttc
     6721 caatattagc tactgttggt gtaataatat ctgctattac aaacatcata cttgattact
     6781 tgtttgtaat tgaatttgga tggggtgtaa aaggtgctgg tattgcaact ggtttgtctc
     6841 aagtcttttc tactattttc ttcttaatac attttttaag aaaaaactct actttgaatt
     6901 tttcaaaatt tagaattgat tttaagacac ttagaaaaat tatatttata ggttttcctg
     6961 attcaactac tgaattaagc tgtggtatag ttgtcttact ttttaactta agtcttacta
     7021 aatatattgg agaaaatgct cttatttatt atagtgtcat aaattatata aatacattag
     7081 tacttatgac tatgatgggt ataactcaag gtatgcaacc acttactagt ttttattatg
     7141 gtgcaggaaa tatagataat gtaaaaaaac tattaaaaat ggggataaaa gccacagtaa
     7201 tagcatcaat ttctgtattt gcagtttgta ttgctttctc agaaccaata gtctctttat
     7261 ttatacaccc agaagaaaca attctattca atgaaggagt tcgtgtcttt aaaatattct
     7321 ctatatcttt cttattggta ggaattaatg taataatttc tggattcttt gtatcagtag
     7381 aaaaaccatc aatttctact gttatatctc ttggtagagg gcttgtaata gttgttttaa
     7441 gtctaatttc aatgatacta atatttggtg gtcaaggaat atggatgact actattgttt
     7501 ctgagtttat atgtcttatt ttatcattag tttttttaaa aaagaatttt tctacattag
     7561 actctaatct aaataaggtt gcatagatga taagagtggc aatatatgaa aatatataaa
     7621 taaaaaatac ctattataca atcttgcaaa cattaaacct ttgcaaatat taggattata
     7681 taataggtat tttatttatg taattatttt atttctaaat atccataacc atcttttact
     7741 aaagttcctt ttacttcata ttctacaggt tttttaaata ttggaccgtc tacgacaaat
     7801 gtatgatact ctccatgttc tccacatggg tctgcaccag catcttttat cttctccaca
     7861 agttctctag ttaatttttt tcctaaaaat tcttctccta gatgctttaa gtttactttt
     7921 gttacaacac tacaaaatcc actgtctata aattcatata ctaaatcctc tctactctct
     7981 tgccataatg gtaaatctac tttcatacca actgcttcac atctatcagt atcccatttt
     8041 ctatggcttt ctatatctat atctccatat gcgcatattg ttgctcccat gtttttagct
     8101 tttattaaag ccctctcaaa actctcttca tatccttcta ctccacactc agcaactaat
     8161 aaaggtaatc ctatattgct acttacttgt ttaagcatat tatattctaa attatgtgtc
     8221 catgattttc catgttcttc acttatagtt gtcaagagtg ccacaggctt atatcctttt
     8281 ttcaacattc tatttaatgc taatatacta tctttaccac cactaaatga cattacaaat
     8341 ttttcatcag ccaattttaa cacctctttc atctaacttt gttttattaa cataattata
     8401 ccaaattttt gagttgcata gcaaattata ttaaattgtt aattcactat aaaaaatgtt
     8461 cctaatatca aaagtataac ccctatgtat ttgtataaat gcattgtttc ttgtcccaaa
     8521 attacgctat caataaaaaa tccaactaaa acttgggaca caagtgttaa tataagtgta
     8581 tttgatactc ctaaatttgg aattgccttt acagtaaaat atattattag acctccaaat
     8641 attcctccta ataacaactt ataattaaaa ccatttatag atactaggca ctttaggtca
     8701 cctgtaacta ttatacttgc caaaaaaaat attgctccta cagttaaact tataaatgtg
     8761 gccactaatg ctgttgtact ttttcctagc tctccattta taaaagcttc aagagctgtc
     8821 gaaccacctg ctaataatgc aaatattatt gcccataatt ttgtcatgga caaacctcct
     8881 tctatataat tctattatat taatagaagt ttatgataca acctattaat taattactaa
     8941 gtatagttta atttttatac actaggcttt atttggatat gaatttctgt tataattgtt
     9001 ctatcgattt tggaggtgat tttatgtaca caaattttaa tatcgatttt aataatttta
     9061 ataaaaaaga aaatgctact aaatttatgt taatgggagt attactcatt atattaggat
     9121 tattatgcct tacatttaag acattaggta taaaattaat ttcttggact tttggtattg
     9181 cacttttatt ctttgcatat ttaaatttaa aaaatattaa tgaactaaaa agatatgcta
     9241 ctaaagaaga aattaaaccc tctattaata ttcaatggat tctaataatt gcatgtatat
     9301 tactttttgt atttcctcaa aaaatacaaa gcatattttc attattatta ggattttatt
     9361 taatctttaa tcaactagta gcacttgtaa atagcaagaa taatccttat tctaaattta
     9421 caacttggaa tatagttaag attctatttg gtatatgcct tatattatct ccactttttt
     9481 tatctagatt tatagtgtcg ataatgtctt ttttcataat attatttgga ctagttctat
     9541 tcttctctgg aaatactgcg agaaaatatt aattattttc tcgcagtatt tttatattgc
     9601 ttaaaataga atttgtttaa gttatttatt tatggtaata tgttttttgt acaatctcat
     9661 tgtacaattt tattagaaat aggtgatttt tttgaataaa tttaaatatg cttttgataa
     9721 taaaaggtat catacttgga attactatct tagaaatact tttggagaaa aagtttttaa
     9781 ggtatctata aatgcaggat ttacttgccc aaacattgat ggaagtattg gttatggtgg
     9841 atgcacatat tgtagcaaag aaggttcggg tgattttgct ggaaatccta aagataatct
     9901 tatcagtcag ttttataata taaaagaaat gatgttaaaa aaatggcctc atgcaaaata
     9961 tattggctac tttcaagcct atacaaatac gtatgctcct cttgaagttt taaaagaaaa
    10021 atatgagact atattagaac ttgaagatgt agtaggtctt tcaatatcta caagacctga
    10081 ctgtttacca gatgatgttg tagaatactt atctgaacta aataaaagaa caaacttatg
    10141 ggttgaactt ggacttcaaa caattcatga ttccacatct aaaattataa atagaggtca
    10201 tgattataaa acatttgttg aaggtgttga aaaattaaag tctaaaaata taaaggttgt
    10261 tgtacacatt ataaatggtc tccctggtga agattataat atgatgatgg aaacagcaaa
    10321 ggcagttggt aaaatggatg tagacggcat aaaaatccat ctattacatg ttataaaaga
    10381 tactcctatg gaaaagatgt tacaaaatgg tatgttaact cttatggaac aagatgaata
    10441 tatcaatctt gtctgcgacc aattagaaat acttcctgaa actatgataa tccatagact
    10501 cactggtgat ggtaaaaggg atgaattagt aggtcctata tggagcctaa aaaaatggga
    10561 aattttaaat caaattgatg acaccttaaa atctagaaat tcttatcagg gttgtaagtt
    10621 tgtttaatac taaataatta ttacaaataa aacaatatct acaaaataat aactatagaa
    10681 ataattatat aaatataatt atttctatag ttatttagcc tttgcaaagc aaaatattaa
    10741 tttgtatatt tacacaataa taataaaaaa tagataatct aaactgtaga tacttatatt
    10801 ttatttgtat tgtccacttt attctataaa ctttataaac agttattttt ttatactaaa
    10861 tataatttgg tataattttt gttagactct acagtgtata ccaataaata atttttaagt
    10921 atctagttct aaatactttc agttttttca atctgcttta acttgctaga cttataaaat
    10981 attcttattt catcataaga aagattaagt gctactactg cagatatcaa catcaacatc
    11041 actccaaata actttcctaa ggaaaaactt tctcctaata tagtgcaacc aattattact
    11101 gaaccaacca actctactga tgccacaaca cctgcaacgg ataactctac tcctttagag
    11161 atacctgttg cgtaaaaaat atatgcaagt gcagctggaa caatacctaa cattaacata
    11221 caaaataaaa catctaaatt attagcataa ttcaatattt cccaaggttt tgaagaagga
    11281 atcataaata tagctccaaa taagaaactg tatactaata ttgttgaact tgaaaattcc
    11341 tttaaagcat ttttactaat agtcggcata agtgcatatg ctattgctga taatatccca
    11401 agtaaaagtc caaaagcgtt taaaccttga aaatccaaac gtccaccagt cactgccata
    11461 atggcgccaa taaaacaaag aataagtgaa aataatttac ttctagtaat attttcttta
    11521 taacatactt ttgagaatat tgctaaaaac aatggtgatg tatatagtaa aactgctgca
    11581 attgatacac ctacaatgtc aattgcttta aaataacata aattaaacat tgcctgacat
    11641 ataataccta ttattactga atatataatt ccttttttgc ttatttttaa gatttgtggt
    11701 gttcttatag atgaatatat tcctaaaact ataaatccaa aaaataatct tgtaaatgcc
    11761 acttgttctg atgccaatcc atttcccatt aaaaactttc caaataatcc aagtgtagcc
    11821 cataataacc ctgctattgc tataaataca taccctttac ttttttctcc cattttaagt
    11881 tcccttctat ttttatttag tcaatttaca ctattctttt taagcaaaat ttatgccatc
    11941 ttaaaccaat atattccgac tctaaaaccc tagtctttcc aacaatacta ataaaacttc
    12001 aattttttta tatttatttt tcacttactt taagttgatt tagacttatt ttaattgtaa
    12061 aatgagttat atataactta tttaaagtta aaatagactg aaatttatat ataaatgtca
    12121 aattttaagt atattaatca attaaactta ttttaatata tcattaaaag gttaggagga
    12181 aaatagaaaa atgaatttaa atttagaatt agatttggaa ttttacaaaa aaatattaga
    12241 agcttcccac gacgaaatat gtgtctctga tgataaagga ataataatat attgtaataa
    12301 agcatttgaa gaaaattatg gtctgaaaaa agaagatatt ttaggaaaaa atgtttcttt
    12361 tttagaagac tctggatatt caacaaaaag ccccatacca gttgtattaa aaacaaaatc
    12421 taaattttca ctagaacaag atactcagac aggaaaaaag ctcatcataa ctgctacccc
    12481 tatatttgat gaaaatggaa acttagaatt tactgttgaa aattgtagag atataactga
    12541 acttaataat ataaaaaata aacttgaaga cacaaaaaaa caagtaaaaa aatataaatc
    12601 tgaagtagaa acactataca gaactgctct tagaattgaa gatactgtaa ttatggatgg
    12661 aatagttatg agaccaatca taaacacagt aaatcatgtt tctaaaacgg atgtaagtgt
    12721 acttttattg ggtgaatctg gaacaggaaa aagctcactt gctagatata tacaccacaa
    12781 cagcaataga gctaatggac catttataac aataaattgt gcaactatat ctccacaact
    12841 tcttgaatct gaactttttg gatatacttc aggtgctttt acaggtgcaa gtactaaagg
    12901 taaagttggt ctcgtagaat tagctaatgg aggaactctt tttttagatg aaataggaga
    12961 tataccacaa aatctacagg caaaattttt gcaattaata caggatagaa cttttactcc
    13021 agttgggagt ttaaaaaaca aaaatgtcga tataaggatt atatctgcta caaatgctga
    13081 tttagtttca aaagtaaaag aaaaaaaatt tagagaagat ttatactata gattaaacgt
    13141 tatagaaata aaattgcctc ctcttagaga aagaagagat aatctagtgg aaattataaa
    13201 atattatttt aatagatact caagtgactt caacctaaat aaaactattt ccaaagaagc
    13261 tatggagact attgcaaatt atagattccc aggtaatata agagaattac aaaatataat
    13321 tcaaaaaatt ttacttactt gtacagataa tcatataaca atacataact taccaaacat
    13381 tcttacaaaa aatataaata tcaccaataa tggaaataaa acacatatat cacaaattaa
    13441 taaagttatt acacctgact caaaatctat taattataaa aataaaaatt ttgatacctt
    13501 aattaaagag tacgaaaaaa atataatttt ggatgcttat gaaaaatttg gtagttctta
    13561 taaagtagcc aaacaccttg aaatttcaca atctaaagcc aataggttga ttagaaagta
    13621 tactaatact taattttaaa tttgataaat aaaaacttaa cactatttac atattgtaaa
    13681 tttggctatt tattggtatt atgaatatgg ttgaaattat ttaaataaat cctattgact
    13741 tagtttcata tatttaccat taaactttta tcataaatat catatcataa taatatattt
    13801 aataagtcta ataaactata cgaggtgtaa atatgttaat gaatacccat tttattatgg
    13861 caaaatcaat acttgataat atagatgaaa ataaaacatt ctttataagc gaaaaaaatt
    13921 ttatatatgg taatataaag cctgatgctt tttctaaata taaattaaaa aaacattatt
    13981 tagatgagtc atttaatatg attgtttcta aaataaacta tctatgtagt cttaatatag
    14041 atagtctttc taaatacttt tcagtaagta gacttagtca ggaactagga gtcatatgcc
    14101 attttttatg tgattttttc tgtgtagctc acagtgaacg ttgggaattt aaacacagtt
    14161 taaataaaca tgttacttat gaaagagaac ttggagcatt tgcaaaagat gttgatttat
    14221 ctaaaattaa atgtggtact ataaatagta gctttgagaa gttttttact aaactttata
    14281 gtgactacaa aaagaaaact gattatatga atgatttaaa attttcagct tatacttgta
    14341 atagtgtcat aaattatata cttgactcta tcttgtctaa tacaataaaa tcatatcata
    14401 tagcaaattg tggttaattt aagaattttt aaacttacaa ttgaatataa ttaaaatgac
    14461 tgctctatag aatataattt tataattctc tagaacagtc attttttata ttaactattt
    14521 atctccatta taacatctat agcacttttt acactatcta ctacataatt tgcactttgc
    14581 tttacatctt cccttgcctt agacattgca aaactatatt taaatccttc taacatacaa
    14641 atatcatttg gagaatcccc aatagtagca acttcatctg gacttacatt taaagttttt
    14701 attacatgct ctatacttcc cctttttgac acacttttag gaactatatt taaagtatat
    14761 gttccagaaa aatatatttc taatacttca ctaaactcgc tttttagata aagttctatt
    14821 ctggataatt cctccaaatt ttcagaacat actcctatat ttcctataag gttatcaaaa
    14881 ctacttcttt tttcaaaatc tgggtcaact ataaaatcac tagaataatg tctaaaagca
    14941 gtggtatcac tacccacaat tacatcaccc ttatatttaa aataaagttg tgcatcactt
    15001 gactcaaatc tatcaatgac ttttttaact aaattatcat ctatcgttct tttaaaaata
    15061 acattataat ctttatcata tataaatgaa ccattttcac atatataata tccattcata
    15121 tctaaactct cttcgataat attacgagct tgtttaaaaa ttctacctgt agcaacatta
    15181 aactgtactc cttttttttc tatttcttca attgctacca tgtcctcttt tgttatagtt
    15241 ccattttcat ataaagttcc atctaaatca caaaatatat gttttatcat aaattaaaag
    15301 cctccaaaaa caattttata ttatactaat attttaatca aaataactta aagcttttct
    15361 aattgtattc tcaattcgtt aagtaaatct ctagtagctc ttaatcttat ctttcttcta
    15421 tctccattaa atatattttt ccttactatt gtttttccat ttatatatat tgcagtataa
    15481 actagtccta caggcttctc tttagttcct ccaccaggtc cagctactcc agttgtagat
    15541 atacctatat tagctttaag tcctttagca acgccctcag ccatttctat tgctgtttgc
    15601 tcacttactg ctccaaactc ttccaaagtt tctcttttta ctcctagtct tttcatttta
    15661 gaatcattag aatatgctac acaaccttca agaaatacag aggatatacc tggatagttt
    15721 ataagagatg aagaaaccaa acctccagta caagattcag ccactgctat tgttagattt
    15781 ttttctacga gtaacttaga aacagcatct tctaatgcag tctccccttc tgatactgaa
    15841 atatctcctt cagtatatac atatttgcca actctgcttt ttattttttc ttctactggt
    15901 tttattaatt taaaggcttc tccttcattt tcagcctttg cagttattct tattgtaact
    15961 tctaattctt ttgcatataa agcaactgtt ggattagtct gctcattaat tatatcgaga
    16021 atttctaatt ctagatttga ctcacctatt ccatataatc ttaaagtctt agatactagc
    16081 atttcatttg taaattgttg taagtaaggt ttgacactct cattaaacat agctttcatt
    16141 tctcttggag gtccaggtaa tactattata aatttgccat cttttcttaa aattgcccct
    16201 ggtgctgtac cattattatt ttttagtact atagcatctt ttggaaagta cacttgtttc
    16261 atattatttt cggccatttt ttttcctgtt ttaacaaaga aagattccaa aacctctagt
    16321 gatggtttat gtagttctaa tttctgacca aagtattctg ccgcagtttc ttttgtcata
    16381 tcatctccag ttggtccaag accacctgta gtaattacaa aatcacttct ctttaggctt
    16441 tcagcaaaac attctaaaag tctttgttta ttatctccaa cagtactctg atggtatact
    16501 tctattccca atgatgcaag ttctttagct aaaaattgag aatttgtatt tactatatct
    16561 cctaataata tttctgttcc tacacttatt atttcagcct tcaaaattta tcactccttg
    16621 atttatagcc attaagacta gtcttaagtt cataacaata attttagcaa aaataataac
    16681 ataaataaag ttaaaaaatc atttaaacat atctttaaac tagagtactg ctctaaaacc
    16741 acacataata caaacttcgt atatagtggt actttcatcg tcaactgtta attcatataa
    16801 ttaagtatgt aaataaaaaa tttactttaa aataataaaa atcaaaatag gagtgtataa
    16861 tatgaaaata gcagttataa tgggtggaat atcatcagag agagaagttt ctttaaattc
    16921 aggaaaagag atatataata accttgataa aaataaatac gaagttgtaa aagtaattat
    16981 agatgataaa aaagatatat ttacaaagat accagaagat atagattttg caatacttgc
    17041 tttacatgga aaatttggtg aagatggatg tatacaatca atacttgaaa ctatggatat
    17101 accatattct ggatgtggac ctctttgcag tgggatgtgc atggataaaa acataactaa
    17161 aaaaatgctt agagattcaa atctacctac tgctccatgg gttctagtaa aatcagttga
    17221 tgaaatagac tatgatgaaa tagataatat aggctaccca gtatttataa aaccaaactc
    17281 aggaggttca agtgtagcca cattctttat tcattctaag gatgaagttg gagaagcagt
    17341 tagaaaaggt ctggaagttg atgaatttgt aatgattgaa aaatatatac caggtggaga
    17401 atatacttca ttcatattaa atggagaagt attccctaca atctctataa aatctgattc
    17461 aggattcttt gattatgaag ctaaatactc agttgaaaaa ggtgcaaaag aggaagttgt
    17521 ttacttagat gaagaacttc aaaaaagagt aaacgaaatt tctgagactt gttggaaaat
    17581 ttttaattgc aaagcatatg taagagttga tatgattata agtgaaggta ttccatatgt
    17641 tcttgaacta aatactcttc ctggaatgac tcaaactagt ttaattccta gaagtgctgc
    17701 tgctagaggt attaagtaca gtgaactatt agataaactt atagaatact ctttaaatta
    17761 gatgaaataa tttgaggtgg taatttgata ttttcaaatc taaaattaaa tgataatgaa
    17821 ccaatttata tacaaattaa aaattatata agtgatatga tatcaaaagg actaattcca
    17881 gacaatagta agctcccttc tacaagggag cttagtcagc ttttacaagt tagccgaaat
    17941 tctgtagttt tgacttatga agaattaaaa tcagacggat taatctactc tatttcagga
    18001 aaaggtacat ttgttaaatc aaagaataag tcttcaaata ctacatggtc attaaactgg
    18061 gattatttag agaatacata ctctaaaaaa gccagtgaat tggatattat aaaaagtgaa
    18121 attccttgga gttctgacct aatttccttt aaaagtattt ctccagacgg agatttattc
    18181 gacatggaag aattaaaaaa atccttttta aatagaattt cactagaagg acataaatta
    18241 ctcaactatg gatatgcaca aggttataag ccccttattg actatttact aaagtatatg
    18301 acaaacaagg gtgttgatat aagtaataaa gatatattaa taacaaatgg attcacagag
    18361 ggttttgata ttgttatgtc atcttttacc caagaaaaag actacataat atgtgaaaat
    18421 cctactcata atacagctat aaaaatcatg aaatctcata atctcaatat cttaggtgta
    18481 gatataaatg aagatggttt ggacttcgat atgctagaat acaacttaat taagtacaaa
    18541 aataaaataa agtttgcata cattacaccc tcttatcaca atcctacagg gattgtaatg
    18601 actcctgaaa atagatacaa attttataat cttatgaaaa aatacaatat agcaattata
    18661 gaggatggat ttaatgaaga attactctac aatagctctc atatatttcc tatctgttcc
    18721 ctagataata tgaataatgg cgttgtatat attggtagtt tttcaaaaat tttatttcct
    18781 ggaatgagaa ttggatggat atttgcagat aaaaaagtaa tcaataagct agaaagtgta
    18841 aaaaggtgta gaaacataca cgtatccttt ttagaccaag gaattttatt tgattactta
    18901 agtaatggag gttttgaaaa atacattaaa aaaatacgta aattctatgg agacaaattt
    18961 aactttgcat atgaatgtgt aaaaaaacat ataaaaacag aatacatctt gggtgatggt
    19021 ggtttgcata tatttataaa actaaaaaat atagatacta gagttctatt agaaaaatgt
    19081 tatgaaaaaa atgttatatt tatgcctgga gatttatttt ttacagataa ctctggtttt
    19141 gatactttaa gacttggatt ttctaggttg tctttcgaag acattgaaat tggaataaaa
    19201 ataattggag aaactataaa tgaaatgttc taggttaact aaatattgtt agcctagttt
    19261 atttttatga tgtataatat atatagttta tatgaatcta atactggggg ataatatgag
    19321 taaaaaatca aaacgaaaaa aaattaataa attaaaatta acgattgtaa taactttatt
    19381 atttcttgct ttattatcta tatatgagat actattctct aatcccaaca aaaaagataa
    19441 tgtaaagagc caagaaacta tgcaaaataa aaataataca acagatgatt ctaaaaataa
    19501 tcaaagctct accaaaaagc aggaagataa caatatcaag cctacttttt catattcttc
    19561 aataccagat aatataaaaa ataaaatgat tggtaaatct atgcctactg atgagcctat
    19621 aagctttgat agtctatctt atttaaaact tacatactat ggatttgatg aaaaaactca
    19681 tcaaggagaa atgatagtaa acagtgagct agctccagaa gtagtggata tttttaagga
    19741 attatacgaa aaaaaatatc caattgaaaa aatcaatcta atagatgact atgatgctgt
    19801 agatgagaaa tctatgtctg ataataatac ctcatcattt tgttatagaa ctatagctgg
    19861 tacaaatgtc atttcaaatc atggtaaagg tcgtgctata gatataaacc cattacagaa
    19921 tccccaagtt tctggaaatg atgttactcc taaagtaagt acagtttatg ctgataggtc
    19981 tagcacaaaa tttggaatga taagaaaagg tgatgattgt tataatgctt ttgtaagcag
    20041 aggttggagt tggggtggat attggaaaaa cccagattat caacattttg aaaaataata
    20101 gattagaaat aaaggtgaat tatatgaatt taatgacatt ggaaaacata agtaaaagct
    20161 attctgaaaa aaaattactt gaaaatatat ctcttggaat aaatgaagga gaaaaaatag
    20221 gacttattgg tgtaaatggt actggtaaat ctaccttact caaaattatt gcaggagttg
    20281 aagaaagtga aactggcaat ataacaaaag ctaatggtat tcggatagaa tatctacctc
    20341 aaaatccaac ttacaatgag aattcaactg tacttgaaca agtatttaat ggaacttctg
    20401 aagaattaaa gctacttggt gaatatcaag aaattctaga agaacttaat tcaaacttta
    20461 cagaggaatt aaataaaaaa ttattatcgg tacacgagaa aatagatgcc ttaaatcttt
    20521 gggatttaga aagtgaagct aaatcagtac ttacaaagct tggtataaat gactttaatc
    20581 aaaaaatagt agaactttct ggtggtcaaa gaaagagagt ctctctggca tcagccctta
    20641 ttacaccttg cgagtttttg atactagatg agcctacaaa ccacctagac aatgatacta
    20701 tagattggct tgaagaatat cttaattcaa gaaaaggttc attgcttatg ataactcatg
    20761 atagatactt tttagatcgt gttacaaata gaattattga actagacaag ggtagattat
    20821 ttagttatga tgggaattat tcaatatttt tggaaaagaa aatggaaaga cttgcgttag
    20881 aagcaagtat tgaagacaaa agacaaaatt taataagaac agaacttgct tgggtaagac
    20941 gaggagctca ggctcgtagc actaaacaaa aagctcgttt acaaagattt gacgaactta
    21001 taaataggga ctcttttaaa cctgatgaaa atctcgatat atctgttgca tcaagtagac
    21061 ttggaaataa aataatagaa atacacaata tatctaaatc atttgaagga aatactgtta
    21121 tagataattt agaatatact ttaactcgta cagatagaat tggcataata ggaaaaaatg
    21181 gtatgggtaa atcgaccctt ataaacatac ttaatggaga aatagaacct gacagtggat
    21241 atatttcaat tggagaaaca gttaaaatag gttgtttttc tcaagacgac tctcatatga
    21301 atattgatta aaaaatagct acgcacactc gtgactttag tcatgaatta gtagctaaaa
    21361 tagtcagcat atagggaaac ttatatgtag agataggata a
//
LOCUS       DAFNNB010000056        21009 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0056, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000056 DAFNNB010000000
VERSION     DAFNNB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 21009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21009
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0056"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(203..1309)
                     /locus_tag="L3M60_001796"
     CDS             complement(203..1309)
                     /locus_tag="L3M60_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HBN6226465.1"
                     /translation="MLDKNRLINNFIDMVRIDSPSNQELEMSKWLVNYLKERNIDAII
                     DDAGEKYGGNTGNVIAYIKGEEGSRPLCLCAHMDQVQPCLGVKPVLDGNVVRSDGTTT
                     LGADDKAGIAAILEALEHVITEKIPHRDIYLCFTICEEAGMHGVKNFNPENLPCKDMV
                     ILDSGGAIGSIAYKAPAQQSIKISFHGKKAHAGIEPEKGLNAILVASHAISNMHIGRI
                     DSLTTSNIGKIEGGGATNIVTDRVTLTAEIRSHIPETLEYELNHMEKCCKDAASKFNT
                     TYTFEHNMSYPSFELSRDSHVFKLSEEAIRQVGIVPNPMVIGGGSDANILANLGYNCA
                     ILSLGMYDVHTVNEYVNIDELYDTAKIVYHMIKL"
     gene            complement(1344..2885)
                     /locus_tag="L3M60_001797"
     CDS             complement(1344..2885)
                     /locus_tag="L3M60_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBN6226466.1"
                     /translation="MNDNVVKKKNFFDRTLNRIETVGNKLPDPVTIFLGLCVLLLILS
                     SVVGSMGISVVHPGTGETITAVNLLTVEQLQILLGNIVSNFQGFAPLGLVLVTMIGAG
                     VCDKTGLMTATIKASVSKIPETRVTLVVMTIGMLANIASDAGTILFPPLAALVYLGVG
                     RHPLIGLFSGYAAVCLGFAANIMISVNDILAASFTVPAAQMIDSGFQANATMNLFFMI
                     ASTFVLILLATWVTEKIVAPRFGKYEGNAKLDVDGSLSDVEKKGLKKAGISILIYVAI
                     IVALSVIGKKPFLADPETGDLLSNNAPLMKGMVPIIALAFFIPGVVYGKTIGKIKKDK
                     DVVSIMASAMSDMGGYIILAFAASQFLQLFTNSNLGLILAVKGGEFLKSAGINGPLLL
                     IGFIILSCFINLFIGSASAKWAILAPIFVPMFMMVGYNPALTQMAYRIGDCITNPLSP
                     LFPYFPIILAFTRKYDKDAGMGTVIANMIPYSISFLIVWTILLILFMIFNIPLGPGIL
                     PSYSM"
     gene            complement(3440..4750)
                     /locus_tag="L3M60_001798"
     CDS             complement(3440..4750)
                     /locus_tag="L3M60_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226467.1"
                     /translation="MNKEQLKSLVIKTIDENREKILKIGQGIYNNPEYGYKEFKTTEA
                     VSNFFEQELNLSVEKNIAVTGCKADANSSKEGPHISILGELDGISCKEHKDANEIGAS
                     HTCGHNIQIAGMLGAAVGLVKSGVLEHLDGKVSFMATPAEEFIELEYREQLKKEGKIT
                     YFGGKQELIKRGYFDDIDISMMFHSSDLGEDKALVGPESNGFVGKKVKFIGKESHAGS
                     APHDGINALNAAMLAINNVNALRETFKESERVRFHPIITKGGDIVNVVPADVHMESYV
                     RARTINGMIDANERINKALMAGGMAVGADVKITEIPGYLPILRYKSLDNLFKNNLEDL
                     GLKGKVIDGGDFTGSFDFGDVSHIMPTLHPMIGGVKGALHTRDFEIVDEDLAYIVPAK
                     AMALTVVDLLFDNAKNAKDILDAFTPVMTKEEYLTFLEKHDKTL"
     gene            complement(4763..5233)
                     /locus_tag="L3M60_001799"
     CDS             complement(4763..5233)
                     /locus_tag="L3M60_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226468.1"
                     /translation="MFANLNLKKSSYQLLVMSVIGVIILIGQRISVGTSIVTALPGMV
                     MLILAAMAAMIIKDLFPKSIFPAFGFATIIGLLLSMPYSPTSEVFLTNTNNINFMAIT
                     TPLLAFAGISVGNKIEALKEMSWKIVIISLIVFTTIFFACASIAHIVLSIQGKI"
     gene            complement(5237..6088)
                     /locus_tag="L3M60_001800"
     CDS             complement(5237..6088)
                     /locus_tag="L3M60_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3100 domain-containing protein"
                     /protein_id="HBN6226469.1"
                     /translation="MEINKKHFIQVFLTCLILVVICEFIGQRTIAIGKASIVLFPMLY
                     AVIIGLIITPDLLGKKIKALKKVIGEKEINIAGEVVGIALVMLGIKYGTTVGPNIDKI
                     IQAGPAFIAQEFGHILAPIVALPLALMFGMKREAVGATASISREPSLGVISEKYGINS
                     PEGSGVLGTYLMGTVLGTIYFSVLGSISIYSGLHPYALGMACGVGSGSMMTAAAGSLS
                     TMVPPEMADTILAYAATSNMMSSITGIIFLVFVSLPFTNFMYKILEPKFSRNKNTKEI
                     KSTKGEC"
     gene            6815..7726
                     /locus_tag="L3M60_001801"
     CDS             6815..7726
                     /locus_tag="L3M60_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HBN6226470.1"
                     /translation="MKINRLTEIIVILLNKKLVTAKELADRFEVSTRTIYRDIETLSM
                     SGVPVYMMKGKGGGISLIEEYSIDKAILSKKDKESLIVALKTLQATKYPEINSVVNKI
                     GSIFGEQNFSNWIEIDFTEWGSNFNEDDKFTKIKEAILRRNIINFNYVNSLSSQTNRT
                     VEPLKLMYKSKTWYLYGFCKLKDDFRIFRISRIRNLSIKEEVFSRKVIEEVCLNDSKV
                     IKENTITLKLRFKEKMLFRVFDDFNKDLITKNEDNTYDVITEFPIGEWIYGYILSFGD
                     NVEVLEPKDVRDNVITRLKELSKIYSL"
     gene            7883..8380
                     /locus_tag="L3M60_001802"
     CDS             7883..8380
                     /locus_tag="L3M60_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HBN6226471.1"
                     /translation="MNYEIVEISEKIVIGISKETTNKDGQAVNDIGELWKKFMGKGIY
                     NAIKGKKSDKTIGLYTDYQGDFTSPYNFVACCEVNSNSDKDKNLEELNTNNTVGEIII
                     SKVIPAGKYAKFVIAGGQKEVGDFWLEFWQMDFDRTYVSDFEEYQSNTFDTNKHEIHI
                     YIGIK"
     gene            9115..9477
                     /locus_tag="L3M60_001803"
     CDS             9115..9477
                     /locus_tag="L3M60_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBN6226472.1"
                     /translation="MGNKILIVDDAIFMRMIIKEALGKSGYNNLVEANDGEEACNIFK
                     YEKPDLMLLDITMPKKDGLEVLREIKKLDSSAKVVMCSAIGQENMIVDAIKLGALDFI
                     VKPFKTETLVKVVNNALK"
     gene            9542..10159
                     /locus_tag="L3M60_001804"
     CDS             9542..10159
                     /locus_tag="L3M60_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HBN6226473.1"
                     /translation="MINYLELDDAHIDALREIGNIGSGNAITSLASMINSNVEVLIPM
                     VKILEYNEATNLLGGPENKVVCILLDMKGDINGMFMFLLDESITQLMLSSLFNKEEVP
                     LDDIEEIEISAIKEIGNIMASSYVNAIASMLNMTISVSIPDICIDMVGAVLNVPMIRF
                     SDVGDKVLFVENKFKMSDNYFTSHILMIPEMSSLREILVKLGLEI"
     gene            10156..10641
                     /locus_tag="L3M60_001805"
     CDS             10156..10641
                     /locus_tag="L3M60_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HBN6226474.1"
                     /translation="MNREIVVNIADMKIAYRPNVLVTYALGSCVGVCLIDKVAGIGGM
                     LHVMLPYSKDAINIENKYKFADTGIKELIKSMENIGANRIYIKAKIAGGAQMFLGNSN
                     SAIASIGHRNVEAVKKTLLGLNIPILAEDTGMNYGRTIRFYTETGELIVDSINKGKKK
                     L"
     gene            10684..11202
                     /locus_tag="L3M60_001806"
     CDS             10684..11202
                     /locus_tag="L3M60_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-binding chemotaxis protein CheW"
                     /protein_id="HBN6226475.1"
                     /translation="MRFPDDPYLDYGLIDEGIKYLKFKVDEQDFGVELEKVIEITGNQ
                     EIIFIPELPTYSKGIINLRGKIIPIIDMRLRLKKDELMITNCMYIVVTEIKDLVVGFV
                     VDRVDDIISLEKSEISPPPRVTIEYSDYFVSGIGELDGKVITLLDLEKLLTDKDEILI
                     DELINGFDCTIM"
     gene            11338..12297
                     /locus_tag="L3M60_001807"
     CDS             11338..12297
                     /locus_tag="L3M60_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6226476.1"
                     /translation="MKNKQQGTILVVDDSVLMCDLIDKALTKDNFKVKKAFNAIEAKK
                     FIEEFIPDIILLDIILPDNSGFDFCKEVKSNARTADIPIIFITSKNADTDIVKGFGVG
                     AIDYMVKPFSMTELKARVIAHLEVKKSQDKLKKINNELESSLEKLNRLVVRDYLTGLY
                     NRRHIINQLMEQRRINKYSETSISLILGDIDDFKVINDTYGHEAGDFVLTIISSIIKK
                     NCRQIDTVSRWGGEEFLIMLTNTPIEDAKILSERIRKEIKEFDFNYKENKIKCTITLG
                     VVEVNSNISLEENISLADKVMYEGKNLGKDCSVVSTMKGLKKI"
     gene            12323..14026
                     /locus_tag="L3M60_001808"
     CDS             12323..14026
                     /locus_tag="L3M60_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HBN6226477.1"
                     /translation="MKREVKLSKLAIVLLVIIVLAVLAGIFSSISSYKAFEKVEKSIN
                     DMNNLRTQSQYIKDTTREISDVLRRYVFTGEKKNKDIYEKELYGKTRESALNKLEKIG
                     LTKSESNKILEAKEISDSLVPTEQSAIKAVESNDKSKAQNIIFSSDYINSQEKMDSLI
                     NEFQEDIVARSNAKIVEVKKELQSLILRTIFILSLLIILTIIEYTVIKLKIVIPINKV
                     EKHFSRIASGDLRTSIDVEESKSEIGLLVSSVKKMQSMLLNYIDLIDSTLTSIAKGDL
                     RVEIDQEFIGDFKSIKISLESIINSFNGDFSEINLSAEQVASASEQVAAGAQALSQGA
                     TEQASSVEELASTINEISDGIKKCADSASLACEVSDQSASQVDFGSRCMDDMMTSMGE
                     ISEKSSEISKIIKTIDEIAFQTNILALNAAVEAARAGQYGRGFAVVADEVRSLAGKSA
                     KAAQNTAILIEEAIKTVENGVKIAKETANALELVVNGVEKTTDYINGISSAVHSQEVA
                     ITQILSGIEQISIVVQMNSATAEESAAASEEMSAQAQILKELVEKFHLKEREVRESSW
                     V"
     gene            14197..16299
                     /locus_tag="L3M60_001809"
     CDS             14197..16299
                     /locus_tag="L3M60_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBN6226478.1"
                     /translation="MRQMLDMFIFETSLLLEQLDEIMIQIEKEKVFTHNNINETFRIM
                     HTIKGSASMMGLDNISALAHSVEDLFYAIREGKIEVLDNDLLFDLVFQSLDFIRRDID
                     ELQSRGCISSDYSNFIEGIEDYVSTLELSSSMKSKDNASLESTDEIEVIDLNFAYREG
                     SDVVKIIFEQGCMMENVRAFMLVFELRDYCEFLDFYPKDVETNAESTEYIKNYGFYIQ
                     FVAKEDNSIVYKIIEENLYVKEYINISKKEIEMKSTSNNNEVENKLKDSNKVSKSYKN
                     NYTEDELQEDVLNEKTDIEKDGLSGENEHLPISKQSIMSVNLEKLDLLQNIVGEIVIM
                     ESMVVNSVNSTGVDIDNFNKSTRQLHKLTDELQDIVMSMRMIQIAGVFQKMHRIVRDM
                     NKKLDKNVELITIGAETEVDKSVIDNLTEPFMHLIRNAMDHAIEPIEERIAKGKKEVG
                     KIVLSAENLGGEAVISISDDGRGIMKDKILAKAKEQGLLKHQTNEYTDEEVNSLIMTP
                     GFSTKESITEFSGRGVGMDIVRKSIEKVGGSIEVKSKPDEGTTFTIKIPLTLSIVEGM
                     KFKVGDSLFTLPISSIRRTFKLTDLNQIITEENKTELITIYGACIPIIRLHKIYNIQT
                     KVTNLMHGNMIIIENENKAVCIFVDEIIGNQQVVVKPFPVYFNRFNIKNKGLSGCTIL
                     GDGSISLIIDADSIIEIY"
     gene            16312..16752
                     /locus_tag="L3M60_001810"
     CDS             16312..16752
                     /locus_tag="L3M60_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBN6226479.1"
                     /translation="MAKDEIIKQVLTFYVNDVIYGIELENVIETIRFQSITYVPCLPT
                     YISGVINLRGRIIPVINMHIKYNLPEADYNERTCIIITKVDEYQVGIIVDRVIDVIPI
                     ENLNLLATTNSNNININKDIKEIAKVEDNSILILDIRKFLINSI"
     gene            16788..17591
                     /locus_tag="L3M60_001811"
     CDS             16788..17591
                     /locus_tag="L3M60_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBN6226480.1"
                     /translation="MIKLTDEEFIVLVNHVKKEYGIDLSKKRALIEGRLYNTMIERKL
                     SSFSQYMNLLFKDKTGNEAINLINRLSTNHTFFMREPQHFEFIQNSIFPFWEENNKIR
                     NINIWSAGCSSGEEAYSIAMTLDDYFGYNKELWNIKIIATDISTNSLEKAKRGIYIES
                     NVNNVPELWKKKYFIDNKDGTFKICDKIRKSVIFKPFNLMNDFSYQHFDLIFCRNVMI
                     YFDLKTREELINKFYNATKKDSFLFVGHAEVINRSNTKYKYVKPAIYKR"
     gene            17631..18227
                     /locus_tag="L3M60_001812"
     CDS             17631..18227
                     /locus_tag="L3M60_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HBN6226481.1"
                     /translation="MGRFNNMVIAIGASVGGTEAILEIIKDLPKSTPGIVVVQHMPAI
                     FTYMYAQRLDKQCIMNVREAKNNDRVEQGNVLIAPGGCQMKLCADKQGYYVTCEKGER
                     ISGHCPSVDVLFDSVAEVAGKNSIGIILTGMGYDGANGLLKMKENGSFTIGQDENSCI
                     VYGMPMVAFDRGSVMLQLPLGEISNCLIRHINILKQKD"
     gene            18333..19190
                     /locus_tag="L3M60_001813"
     CDS             18333..19190
                     /locus_tag="L3M60_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HBN6226482.1"
                     /translation="MKFFKVDTKAKVLRYVFVLTLTIMAVLAIKHDYKYNNLKNASIN
                     LNNKEAVSVMSNQSLPKGNTNSKQTLSVSTIGSKTIVKNDEYLESTINMPVITNSNKI
                     VERSTNDKIKNDIFEFYNKSYKEAKQYLKDNPDEKNKFVANVDFELKKNTDSALSIKV
                     RYYTYSGGAHGFYQDTAYNVDMRTGKFLELMDLFKDNTKYKEVIDEEIKRQIAELEKK
                     DEENIGIYNFKGIKENQNFYLQDDNLVIYFDLYDITPYAAGIPEFSINKKLISSMLKP
                     EYVDLFDLK"
     gene            19258..20247
                     /locus_tag="L3M60_001814"
     CDS             19258..20247
                     /locus_tag="L3M60_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HBN6226483.1"
                     /translation="MLLLKKDDIKKVFTMRDAIEADKEAFRIYCEGKSVNPLRTNIPV
                     PSQDASMLFMPGYVEELGCGGIKIVSVFPKNAQKGKPVIPATVLLLDGETGEVSAVLD
                     GTYVTQIRTGAASGAAIDVLAKKDSKIGALIGVGGQGEPQLEAMIEARNLDEVRVFSR
                     NKENREKFVEEMNLKLSKYNTKIVAVESSDEAIDNADVIVLATPSKQPVFNGNLVKKG
                     ALISAVGSYMPDMQELCPICLTRASKIFFESTDAVLSESGDILIPLKEGKISKDDFSG
                     DLGNVINGTIPGREDDDEIIVFKTVGIGVQDVVTAKRIYDKAKENGIGMDWKW"
     gene            20339..20950
                     /locus_tag="L3M60_001815"
     CDS             20339..20950
                     /locus_tag="L3M60_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226484.1"
                     /translation="MKMVKKLILSSIMALVLVLTIGCSNAEEKVKSNFEANLAALEMY
                     KYDEKGIADALTEAEYKQIQTYNSKDKVSMIAQLTPLYGKENAEKIYETIVKLIQGVE
                     KTVTVESSSKDKITLKVVSKALDTSKVMSEANNLVKSYVTDEKNYDKKEKEVQDDMAK
                     MIIDLYNKKPTKEVTTMITYTKKDGKWQPPSNIQKLCEGIYTI"
BASE COUNT         8184 a         2480 c         3522 g         6823 t
ORIGIN      
        1 tatataaaaa actaaagctt attatgaaat atattacaac agattactat ataaaaaact
       61 aaagcttatt attaaaaatg ctagaataaa ttactatata aaaaactaaa acctatgtaa
      121 agcaactttt taatatataa cttgtaaagt tgaagataca taggttttta tttttataat
      181 ctaaagagtt atattatgat atttataatt taatcatatg ataaactatt ttggcagtat
      241 catataattc atctatattt acatattcat taaccgtgtg cacatcatac atacctagac
      301 ttaatatagc acaattatat ccaagattag ctaatatatt tgcgtcactt ccaccaccaa
      361 ttaccattgg atttggaaca ataccgactt gtcttattgc ttcttcactt aacttgaaaa
      421 catgactatc tctactaagt tcaaaactag ggtaagacat attatgttca aaagtataag
      481 tagtattaaa tttagatgca gcatccttac aacatttttc catatgatta agctcatact
      541 ctaaagtttc agggatatga gacctaattt cagcagtcaa tgtaacccta tcagttacta
      601 tgtttgtagc tccaccacct tctattttac ctatatttga ggtagtcaat gaatctattc
      661 tgcctatgtg catattgctt attgcatgag aggctactaa tatagcattt aacccttttt
      721 caggttctat accagcatgt gctttctttc catgaaaaga gatttttata gattgttgag
      781 caggagcttt gtatgctatc gaaccaatag ctccaccaga atctaaaatc accatatctt
      841 tacaaggcaa gttttcagga ttaaagtttt tgacaccatg cataccagct tcctcacaga
      901 ttgtaaaaca aagataaata tctctatgtg gtattttttc tgtaataaca tgttctaaag
      961 cttcaagtat agcagctatt ccagctttat catctgcacc aagtgtagta gttccatcac
     1021 ttctaactac attaccatct agtactggct ttacacctaa acaaggttga acttggtcca
     1081 tatgtgcaca taaacaaagt ggtctacttc cttcttcacc ctttatatag gcaatgacat
     1141 tacctgtgtt accaccatat ttttcacctg catcatctat aatggcatct atatttcttt
     1201 cttttaagta gtttaccaac cacttagaca tttctaattc ttgattagat gggctatcaa
     1261 ttctaaccat gtctataaag ttatttataa gtctattttt atccaacata aattatttct
     1321 ccttttagaa agatttaaat aaattacatg ctatatgaag gaagtattcc aggtcctagt
     1381 ggaatattaa atatcataaa caatatcaat agtatagtcc acactattaa gaatgatata
     1441 gaatatggta tcatattagc aattacagta cccataccag catctttatc gtattttctt
     1501 gtgaatgcta gtataatagg gaagtacgga aataatgggc taagtggatt tgttatacaa
     1561 tcacctatac gataagccat ctgagtaagt gctggattgt atccaaccat cataaacatt
     1621 ggtacaaata taggtgcaag aatagcccac tttgcagatg cactacctat aaataaattt
     1681 ataaagcatg ataatataat gaatcctatc aataataaag gtccatttat tcctgctgat
     1741 tttaaaaatt ctccaccctt tacagctaat atcagaccca aattactatt tgtgaataat
     1801 tgtaaaaatt gagaagcagc aaatgctagt attatatatc cacccatatc actcattgca
     1861 ctagccatta tagagactac atccttatcc ttctttattt tacctatagt ttttccgtat
     1921 acaacaccag gtataaaaaa tgctagtgct attataggta ccataccctt cataagagga
     1981 gcattgtttg atagtaaatc tcctgtttcc gggtcagcta agaatggttt tttaccgatt
     2041 acactaagag ctactataat agctacatat attaatatac ttataccagc ttttttaagc
     2101 cctttctttt ctacatcact aaggcttcca tcaacatcta attttgcatt tccttcatat
     2161 ttaccaaatc taggagctac aattttttct gttacccatg ttgccaaaag tattaggaca
     2221 aaagttgatg ctatcataaa aaataagttc attgttgcat tggcttgaaa tccagaatca
     2281 atcatctgag ctgctggaac tgtaaaactt gcagcaagta tatcattgac gcttatcata
     2341 atgtttgcag caaatcctag acagactgct gcatatccag aaaataaacc tataagtgga
     2401 tgtctaccaa caccaagata tacaagtgca gctaatggtg gaaatagtat tgtacctgca
     2461 tctgaagcta tattggcaag catacctata gtcattacaa caagagttac tcttgtttca
     2521 ggaatcttgc ttactgatgc tttgattgtt gctgtcataa gtcctgtttt atcacataca
     2581 ccagcaccta tcatagttac taaaactaga cctaaaggag caaaaccttg gaaattgcta
     2641 actatgtttc ctaaaagaat ttgaagttgt tctacagtca ataaatttac agcagttata
     2701 gtttctccag taccaggatg aactactgat atccccatag aaccaactac actagatagt
     2761 ataagtaaaa gtacacataa ccctaaaaat atcgtaaccg ggtctggtag tttattacca
     2821 acagtttcta ttctgtttaa tgttctgtca aaaaagtttt tcttcttaac tacgttatca
     2881 ttcataatat cctccctaat atttaaaaat ataattaaaa aataagtatt gtggttaagc
     2941 tttcatgtaa attctgcttg ttgatttaag acgtgtccac aatatgtcct aaaaaaagta
     3001 taaaataaac tttttcctat gtcaatgcag ttttctgaat tttctggttg agtagtgtta
     3061 gttaaatata ctttaagtag atttatgatg aattgtattt aatatatctt tgcagtatat
     3121 aatctttaaa attaatttta aaacaaaatt ttataaaaag ctagtggaag attaagttat
     3181 tgattttatt gagttttatt aaagataata ttttttattt taattcctat attagggtat
     3241 ttattttgtt taaaagttaa aaggacaaat agtaggcaga tataacaata aatgataata
     3301 aggatatctt tctcaaaata gatatagact tttttataac taaaactata aaaatatatt
     3361 tgatttcata caaaaaagag tgtctaataa actaaaaaat tcatctttag acactcaatt
     3421 ttgtattata aaaatagact tataaagttt tatcgtgttt ttctaggaat gttagatatt
     3481 cttctttagt cataactgga gtaaaggcat ccaatatatc tttagcattc ttagcattgt
     3541 caaaaagaag gtctacaact gtaagtgcca ttgctttagc aggaactatg taagctaaat
     3601 cttcatcaac tatttcaaaa tcccttgtat gaagagctcc ttttacacca ccaatcatag
     3661 ggtgaagagt aggcattata tgagacacat ctccaaaatc aaaagaacct gtaaaatctc
     3721 caccatcaat aacttttccc tttagaccta aatcttcaag attgttttta aataaattat
     3781 caaggctttt gtatcttaat attggaagat atccaggaat ttcagttatc ttaacatcag
     3841 ctccaactgc catcccacca gccatcaaag ccttatttat tctttcatta gcatcaatca
     3901 taccatttat agttctagct ctaacatatg attccatatg aacgtcagca ggaacaacat
     3961 ttactatatc tccacctttt gtaatgatag ggtggaatct aactctttca gactctttaa
     4021 aagtttctct aagtgcattt acattattta tagcaagcat tgctgcattt aaagcattta
     4081 ttccatcatg tggagcagaa ccagcatgag attctttacc tataaattta actttttttc
     4141 caacaaatcc attactttca ggaccaacta gagccttatc ttctcctaaa tcagaagaat
     4201 gaaacatcat agatatgtct atatcatcaa aataacctct ttttattaac tcttgttttc
     4261 caccaaaata agttattttt ccttcctttt ttaattgttc tctgtattcc aactcaataa
     4321 actcttcagc tggagtagcc ataaaagata ctttaccatc taaatgttct aaaacaccac
     4381 ttttcactaa accaacagcg gcacctaaca ttccagctat ttgaatatta tgtccacaag
     4441 tatgagaagc acctatttca ttagcatctt tatgttcttt acatgatata ccatccaatt
     4501 ctccaagtat agatatatga ggaccttcct tgctagaatt tgcatctgct ttacatcctg
     4561 taactgctat gtttttttct acagataagt ttaattcctg ctcaaaaaag tttgaaacag
     4621 cttccgtagt tttaaattct ttatatccat attctggatt attgtatatt ccttgaccta
     4681 tttttaatat tttttctcta ttttcatcta tagtttttat tactagagat tttaattgtt
     4741 ctttattcat aataaattcc tcctatattt taccttgaat acttaaaact atatgagcta
     4801 tactcgcaca tgcaaagaat atagttgtaa atacaataag tgagattata actattttcc
     4861 atgacatttc ttttaaagct tctattttgt ttccaacaga aattcctgca aaagctagta
     4921 gtggagtagt tatagccata aagttaatat tattagtgtt agttaagaat acttcagatg
     4981 ttggactata tggcatacta agtaaaaggc ctattatagt tgcaaatcca aaagctggaa
     5041 agattgattt aggaaataaa tcttttataa tcatagccgc catagcagct aatattagca
     5101 tcaccatacc aggaagagct gtaactatag atgtaccaac gctaattctt tgtccaataa
     5161 gaattataac cccaataaca gacataacca ataattgata tgaacttttc ttcaaattta
     5221 aattagcaaa catttgttag cactctcctt ttgtagattt aatttcttta gtatttttat
     5281 ttctcgaaaa tttaggttct aaaatcttat acataaaatt agtaaatggt aaagatacaa
     5341 aaactaagaa tataattcct gttatgctag acatcatatt gcttgttgca gcataggcaa
     5401 gtattgtatc tgccatttca ggtggtacca tggtagacaa tgaaccagca gctgccgtca
     5461 tcatacttcc actaccaaca ccacaggcca ttcccaaagc atatgggtga agaccactgt
     5521 agatagatat tgaacccagg acactaaagt atatagtacc aagaactgtt cccattaaat
     5581 atgtaccaag tacaccacta ccttctggag agtttatccc atatttttct gatataactc
     5641 ctaaagaagg ttcacggctt atacttgcag tagctccaac agcttctctt ttcattccaa
     5701 acattaatgc aagaggtaat gcaacgattg gtgcaagtat atgaccaaat tcttgggcaa
     5761 tgaatgctgg tccggcttga attatcttgt caatgtttgg tccaactgta gtaccatact
     5821 ttataccaag cattactagt gcaattccaa caacttcacc agcaatattt atttcttttt
     5881 ctccaattac ttttttaagt gctttaattt ttttacctaa taaatctggt gttattataa
     5941 gtccaattat aactgcataa agcataggga acagtactat agaagctttt ccaatagcta
     6001 tagttctttg ccctataaat tcacatatta ctactaagat taagcaagtt aaaaatactt
     6061 gaataaaatg ttttttatta atttccatga tttcctcccc aaaagaataa atattgtact
     6121 tatttgaaat gataaaatac gttttcttaa gtataatacg ttttcctaaa tgaaataaaa
     6181 aaactatttc aaataagttt cagaaatgtc aataacatgt ccttaattaa ggataaaaca
     6241 aaaatttaac attgtcaacg aaaaatatct aaaatattac atattttgaa tatattgtaa
     6301 tatggcttta ttatgaaaaa gttgtttaaa tattgtatga gtaataatta aaaagcttca
     6361 aaattagtga tataaaattt aaaaataata acatagaatt gagaaatagt aaattgaaat
     6421 tttaaaataa atgagtgaga gtggtttaga aagcattgaa atgaatagta taataaaaaa
     6481 taaatactat ggaaagactt tgaattaatt gtaagtatta agtatagata ctaatttttg
     6541 atatagaaat ttaataaagt aaatgataat aagcaagtta agtaaaatga tgacaaatga
     6601 tattataata agggaattga catagcaata agtaaattaa gtaagatagt aataagtaaa
     6661 ttaagataat aatatccaaa gtgagaaaga taatagatag taaataaaat catataagaa
     6721 taatctagat aagattctaa gattttattt gcttaaaaat tggtataatt aatttagatt
     6781 aaataagttt aataatttta gtaggtggta atgaatgaaa ataaatagat taacagaaat
     6841 aatagtaatt ctattaaata aaaaactagt aacagctaaa gagttggcag atagatttga
     6901 agtttctaca agaaccatct atagagatat agaaactctg tctatgtcag gagtaccagt
     6961 atatatgatg aaaggcaaag gaggaggtat atctttaata gaagaatatt ctatagataa
     7021 ggctatattg tcaaaaaaag ataaagagag tttaatagtg gcgctgaaaa cattacaggc
     7081 gactaagtat ccagaaataa attcagtagt aaataaaatt ggttcaatat ttggagagca
     7141 gaatttttca aactggattg aaatagactt tactgaatgg ggaagcaatt ttaatgaaga
     7201 tgataaattt acaaaaataa aagaagctat tttaaggcgt aatattataa attttaatta
     7261 tgtaaatagt ttgtcaagcc agacaaacag aactgtcgaa ccacttaagc ttatgtataa
     7321 gagtaagact tggtacttgt atggattttg taagctaaaa gatgatttta ggatatttag
     7381 aattagtaga ataaggaatt tatctataaa ggaagaagtg ttttcaagaa aggtaataga
     7441 agaagtatgc ttgaatgact ctaaagtaat taaggaaaat actataacgt tgaaattgag
     7501 gtttaaagaa aagatgcttt ttagagtatt tgatgatttt aataaagatt taataactaa
     7561 aaatgaagat aatacatatg atgtaattac ggaatttcct attggtgaat ggatttatgg
     7621 atatatttta tcctttggcg acaacgttga agtacttgaa cctaaagatg ttagggataa
     7681 tgtaataact agactaaagg agttgtctaa aatttattcc ttataatata atgctagatt
     7741 aaaaagttta ttaggtatat tataatatct cactgagttt aattatatta aaaatatgac
     7801 ataaaggtgt cgtatattat aaattataat tgaattagtt caaagtaaaa tatattaata
     7861 aaaacaaagt gaggtataaa gtatgaatta tgaaatagta gagataagtg agaaaatagt
     7921 tataggaata tcaaaggaaa ctacaaataa agatggacaa gcagtaaatg atatagggga
     7981 attgtggaaa aaatttatgg gcaaaggtat atataatgct ataaagggta agaagagtga
     8041 caagacaata ggtctatata cagattacca gggtgatttt acaagtccat ataactttgt
     8101 tgcatgttgt gaagtaaata gtaattctga taaagataag aatttagaag aattaaatac
     8161 taataatact gttggagaaa ttattattag taaagttata ccagcaggta agtatgcaaa
     8221 gtttgttata gcaggaggtc aaaaagaagt aggagatttt tggttggagt tttggcaaat
     8281 ggattttgac aggacttatg taagtgattt tgaagagtat caaagtaata cttttgatac
     8341 aaataaacat gaaattcata tttacatagg aataaagtag atttgttact attatattga
     8401 atgaaggaag tatcgagtat agtagattta ttatagaaag tttaattatt aaaaaagcag
     8461 taaatctttt acagatttac tgctttttta ataattaaca gtatcattat cactttgaat
     8521 atttagacat attcttcatt taatattgtt tgttgtaaaa tgatacttgt aaatataatg
     8581 acttgtcatt taatttgaaa tatgaaaaaa agcattataa gtatgtgata acacttttgg
     8641 atttatgagt atgcaatttg aaaaatataa aaaataatgt ataaagctaa atataattta
     8701 atcgataata catatataga aatataatta agtaaataaa caactatcta ttatatatta
     8761 agaaagtcaa atcttatata aatctacaga aatgatttta atgaatctga aaattatttt
     8821 tatacaaata taaataaggt ataaagttat gttttattat aatttgatta cttttgatgt
     8881 tttatacaat tatattttta ataactcgtc aaaacaccaa acatatatta caatagatat
     8941 ataagttttt ttgttgaaaa attatgttaa gagaattaag cataatatga cataagtaat
     9001 tacttttatt tatcaatgaa atgtatttat tttgtagcga aaaatagtaa gatagtatag
     9061 ataataactg aaaataaaag cgtttattgt tgtacatatg gaggagttag ttttatggga
     9121 aataaaattt tgatagtaga tgatgctatt tttatgagaa tgataattaa agaggctctg
     9181 ggtaaaagtg gctataataa tttagtagaa gcaaatgatg gagaagaagc gtgtaatata
     9241 tttaaatatg aaaagcccga tttgatgttg cttgacatta caatgccaaa aaaagatggt
     9301 ttagaggtgc taagagaaat aaaaaagttg gattcaagtg ctaaggttgt aatgtgctct
     9361 gcaataggac aagagaatat gattgtagat gccataaaac ttggagcatt agattttata
     9421 gtgaaaccct tcaaaacaga aacattagtt aaagttgtaa ataatgcttt aaaataaaag
     9481 tgtaaatata tactgataca ataaaaatca aataaattta ataatagagg gaggggttta
     9541 tattataaat tatttagaac ttgatgatgc tcatatagat gctcttagag aaattggtaa
     9601 tataggctct gggaatgcaa ttacttcact tgcatccatg ataaatagta atgtagaagt
     9661 attaatacct atggtcaaga tacttgaata taacgaagcg acaaatttgc ttggtggtcc
     9721 agaaaacaaa gtagtatgta ttttactcga tatgaaaggc gatattaatg gaatgtttat
     9781 gtttttgttg gatgaatcaa taactcaact tatgctaagt tctcttttta ataaagaaga
     9841 agttccttta gatgatattg aggaaattga aatttcagca attaaagaaa taggaaatat
     9901 tatggcaagt tcatatgtaa atgctattgc atctatgtta aacatgacta taagtgtttc
     9961 aattccagat atatgtattg atatggttgg agctgttcta aatgtgccta tgataagatt
    10021 ttcagatgtt ggtgacaaag ttttatttgt agaaaataag tttaaaatga gtgataatta
    10081 ctttactagt cacattttga tgattccaga aatgagctca cttagagaaa tactagtgaa
    10141 gttgggatta gagatatgaa tagggagata gtagtgaaca ttgcagatat gaagatagcc
    10201 tatagaccaa atgttttagt tacttatgca cttggttctt gtgtaggagt atgtttgata
    10261 gataaagtag ctggaattgg tggaatgttg catgttatgt tgccatatag taaagatgct
    10321 atcaacattg aaaataagta taaatttgct gacacaggaa taaaagagct cattaaatct
    10381 atggagaata taggtgcaaa tagaatatat ataaaagcaa aaattgctgg aggggctcag
    10441 atgtttttag gaaatagcaa ttcagcaatt gcaagtatag gacacaggaa tgttgaggca
    10501 gtaaaaaaaa ctcttttagg attaaatatt ccaatattag ctgaggacac aggtatgaat
    10561 tatggaagaa caatacgttt ttatactgaa acaggtgaat taattgtaga ttcaataaat
    10621 aaggggaaaa agaaactata atatagattt tatagaaagt atattttagt agggaggtat
    10681 taaatgagat ttccagatga cccatattta gattatggat taattgatga aggaattaag
    10741 tatttaaagt ttaaagtaga tgaacaagat tttggggttg aacttgaaaa agtaatagaa
    10801 attactggaa atcaagaaat tatatttatt ccagaactac ctacatattc taaaggtata
    10861 ataaatctaa gggggaaaat tattccaata atagatatgc gtttgagact taaaaaagat
    10921 gaattaatga ttacaaattg tatgtatata gtagtaactg aaataaaaga cttagtagtt
    10981 ggatttgtgg ttgatagagt tgatgatata ataagcctag aaaaaagtga aatatctcct
    11041 ccaccaagag ttactataga atattcagat tattttgtaa gcggtattgg agaattagat
    11101 ggtaaagtaa ttactttact agatttagag aaacttttaa cagataaaga tgaaatttta
    11161 atagatgaat taatcaatgg gtttgattgc actatcatgt aatataggga tgtagttgga
    11221 atgttattat aaaaagattg ttatgagaac atttaagaat atagtggtat aattattaag
    11281 tataaaaatt ttaaataatt aaaatttaaa tatattatgt ttaagagagg tttgtatatg
    11341 aaaaataagc aacagggaac aattttagtc gttgatgata gtgttctgat gtgtgactta
    11401 atagataaag cacttacaaa agataatttt aaggttaaaa aagcatttaa tgcaatagag
    11461 gcaaaaaagt ttattgaaga atttatacca gatattattt tgcttgatat aattttacca
    11521 gataactctg gttttgattt ttgtaaggaa gtaaaatcta atgctcgcac agcagatata
    11581 cctataattt ttataacttc aaaaaatgca gatacagaca tagtaaaagg atttggtgta
    11641 ggagcaattg attatatggt gaaacctttt agtatgacag aattaaaagc tcgtgttata
    11701 gctcatcttg aagtaaagaa atctcaagat aaattaaaaa agataaataa tgaactggaa
    11761 tcttctcttg aaaaattgaa tagactcgtt gtaagagatt atcttacagg tctttacaat
    11821 aggcgacata taattaatca acttatggag caaagaagaa ttaataaata tagtgaaaca
    11881 tcaatatccc ttatccttgg tgatattgat gactttaagg ttatcaatga tacttatgga
    11941 catgaagcgg gggacttcgt gttaacaata atatcaagta taataaaaaa gaattgtaga
    12001 caaattgaca ctgtatcaag atggggtgga gaagagtttt tgataatgct cacaaatact
    12061 ccaatagaag acgctaagat actttcagaa agaatccgta aagaaataaa ggagtttgat
    12121 ttcaactata aagaaaataa aataaagtgc actattacat taggagtagt agaagtaaac
    12181 tcaaatatat cattagaaga aaatatatcc ttagcagata aagtaatgta tgagggtaaa
    12241 aacttaggaa aagattgtag cgtagtgagt acaatgaaag gattaaaaaa gatataaact
    12301 tactttgtaa aggagtacag atatgaaaag agaagtaaag ctttctaaat tagcaattgt
    12361 attgctggtt attatagtat tggctgttct agcagggatt ttttcttcta tttcatctta
    12421 taaagcattt gaaaaagtag aaaaaagtat taatgatatg aataatttga gaactcaaag
    12481 tcagtatata aaagatacaa ctagagagat aagtgatgta ttaagaagat atgtatttac
    12541 aggagaaaaa aagaataaag atatttatga aaaagaattg tacggaaaaa caagagaatc
    12601 agctttgaac aaattagaga aaattggtct tacaaagagc gagtcaaata aaatcttaga
    12661 agcaaaagaa atatcagatt ctttggtacc aacagaacaa agtgcaataa aggcagtcga
    12721 aagtaatgat aagagtaaag ctcaaaatat aatttttagt agtgattata taaactccca
    12781 agagaaaatg gattctttaa taaatgaatt tcaagaggat atagttgccc gttcaaatgc
    12841 caaaattgta gaagtaaaaa aagaattaca aagtctaata ttaagaacaa tatttatact
    12901 ttctctctta ataatactga caataataga atatacagta ataaaattaa aaatagttat
    12961 tcctattaat aaagtagaaa aacacttttc aagaattgca tctggggatt taagaacaag
    13021 tatagacgtt gaagaaagta aatctgaaat agggctgtta gtgagttctg tcaaaaagat
    13081 gcaaagtatg cttttaaatt atatagacct tatagacagt acattaacta gtattgcaaa
    13141 aggagattta agagtagaaa tagaccaaga atttataggt gattttaaat caataaaaat
    13201 atctttagaa agtataataa actcttttaa tggtgatttt tctgaaataa atttatcagc
    13261 agagcaagtt gcaagtgcat cagaacaggt tgcagcaggt gcacaagctc tttcacaggg
    13321 tgctactgaa caagctagtt cagtagaaga attggcttcc acaattaatg aaatatcaga
    13381 tggaattaag aagtgtgcag atagtgctag tcttgcatgt gaggtatctg accaatctgc
    13441 atcacaagtt gattttggaa gtagatgtat ggatgatatg atgacatcaa tgggtgaaat
    13501 aagtgaaaaa tcgagtgaaa tatcaaaaat aattaaaaca atagatgaga tagcatttca
    13561 aactaatatt cttgcattaa atgcagctgt tgaagcagca agagcaggtc aatatggtag
    13621 gggatttgca gttgtagcag atgaagttag gtctttggct ggaaaaagtg caaaagcagc
    13681 tcaaaataca gctattctca tagaagaagc tattaaaaca gtagaaaatg gtgtcaaaat
    13741 tgctaaagag actgcaaatg cattggagtt ggtagtaaat ggtgtagaaa aaactactga
    13801 ttatattaat ggtatttctt cagctgtaca tagtcaagaa gtagcaatta cacagatttt
    13861 atctggaata gagcaaattt ccatagtagt ccaaatgaac tcagcaacag cagaggaaag
    13921 tgcagcagcc tcagaagaga tgtctgctca agcgcaaatt ttaaaagaat tagtagaaaa
    13981 atttcatcta aaggaaaggg aagtaagaga atcatcatgg gtttaaatta tgaggtgcat
    14041 atataacgaa ataagagagt tattgtatat ttaaattatg aggtacatat atagtgaaat
    14101 aatagaatca tcaaggattt aaattatgag atgcatatat aatagaaaat tatttgtagg
    14161 tcgtaggtga taaatgtgaa ttatattgaa ccgagtatga gacaaatgtt agatatgttt
    14221 atatttgaga caagtctatt actcgaacaa ttagatgaaa ttatgattca aatagaaaaa
    14281 gagaaagtat ttactcataa caatattaat gaaactttta gaataatgca tacgataaaa
    14341 ggttcagcat ctatgatggg gttagacaat atatctgctt tagcacatag tgtagaagac
    14401 ttgttttatg ctatacgtga aggtaaaata gaagttttag acaatgacct attatttgat
    14461 ttagtttttc aatctttaga ttttataaga agagatatag atgagttaca atcaagaggt
    14521 tgtatatctt ctgattactc taattttatc gaaggaattg aagattatgt gagtactttg
    14581 gaattatcaa gttctatgaa atcaaaggat aatgcaagtt tagaaagtac agatgaaata
    14641 gaagtaatag acttaaattt tgcatacaga gaaggttctg atgtagtaaa aataatcttt
    14701 gaacaaggtt gcatgatgga aaatgtgaga gctttcatgc ttgtatttga acttagagat
    14761 tactgtgaat ttctagattt ttatccaaaa gatgtagaaa caaatgcaga gtctacagaa
    14821 tatattaaaa attatgggtt ttatatacaa tttgttgcta aagaggataa ttcaatagtt
    14881 tataaaatca tagaagagaa tctctatgta aaagaatata taaatattag taaaaaagaa
    14941 atagaaatga aaagtacatc aaacaataat gaagtagaaa acaaattaaa agatagtaac
    15001 aaagtaagta aaagttataa aaataactat acagaagatg agttacaaga agatgtttta
    15061 aatgaaaaaa ctgatataga aaaagatggt ctaagtggag aaaatgaaca tttaccaata
    15121 agtaagcaga gtattatgag tgtaaattta gaaaaattgg acttacttca aaatatagtt
    15181 ggagaaattg taattatgga atcaatggtt gtaaatagtg taaattctac aggtgttgat
    15241 atagataatt tcaataagtc aacaagacaa ttacacaagc ttacagatga attgcaagat
    15301 atagtaatgt ctatgcgtat gattcaaatt gctggtgtat ttcaaaaaat gcatagaatt
    15361 gtacgagaca tgaataaaaa attagataaa aatgtagagt taataactat aggagcagag
    15421 acagaggtag ataagagtgt aattgataat ttaacagaac cttttatgca ccttataaga
    15481 aatgctatgg accatgcgat agagcctatc gaagaacgta tagctaaagg caaaaaagaa
    15541 gtgggaaaga tagttttatc tgctgaaaat ttaggtggag aagctgtgat aagtatttca
    15601 gatgatggta gaggcattat gaaagacaag attttagcaa aagctaagga acaaggtctc
    15661 cttaaacatc aaaccaatga gtatactgat gaggaggtca attctttgat tatgactcca
    15721 ggtttttcta caaaagaatc tataactgaa ttttctggta gaggagtggg tatggacatt
    15781 gttcgtaaaa gtattgaaaa ggttggaggt tcaattgaag taaaaagtaa accagatgaa
    15841 gggacaactt ttactataaa aataccacta actctttcta tagtagaggg tatgaagttt
    15901 aaagttggag attcactatt tactttacca atatcatcta taaggagaac atttaaactt
    15961 acagacttaa atcaaataat tacagaagaa aataaaacag agttaattac aatatatgga
    16021 gcatgtattc ctattataag attacataaa atatataata ttcaaacaaa agtaaccaat
    16081 ctgatgcatg gtaatatgat aattattgaa aatgagaata aagcagtatg catttttgtt
    16141 gatgaaataa taggtaatca acaagttgtt gtgaaaccat ttccagttta ctttaaccga
    16201 ttcaatataa aaaataaggg tttatcaggt tgcacaatac ttggggatgg aagtataagc
    16261 ttaattatag atgcagatag cattatagaa atatattagg ggatttaaag tatggctaaa
    16321 gatgaaatta ttaaacaagt attaacattt tatgtcaatg atgttattta tggaatagaa
    16381 ttagaaaatg tgattgagac cataagattt caatcaataa cgtatgtacc atgtcttcca
    16441 acatatataa gtggagttat taatttgcga ggaagaataa taccagttat taatatgcat
    16501 ataaagtata atcttcctga agctgattat aatgagagaa cttgtataat aattacaaag
    16561 gttgatgagt atcaagttgg aattattgta gatagagtta ttgatgttat tcctatagaa
    16621 aatctaaatt tgctagctac aactaattct aacaatatta atattaataa agatataaaa
    16681 gaaattgcga aagttgaaga taattctata ttaattttag atattcgaaa gtttttgatt
    16741 aatagcatat agttagtttt gataaattag caggaggaag tttttgcatg ataaaactca
    16801 cagatgaaga atttatagtt cttgtaaacc atgtaaaaaa agaatatggt attgatttaa
    16861 gcaaaaaaag agcgttaatt gaaggtcgct tatacaatac aatgatagag agaaaactta
    16921 gttcttttag tcaatatatg aatctattat ttaaagataa gacaggaaat gaagctataa
    16981 acctaataaa tagattgagc accaatcata cattttttat gagagaacct caacactttg
    17041 agtttataca gaatagtata tttcctttct gggaagaaaa taataaaata agaaacataa
    17101 atatatggag tgcaggctgt tccagtggag aagaagcgta ttcaatagct atgactttgg
    17161 atgattattt tggatataat aaagagttgt ggaacattaa aatcatagcc acagatattt
    17221 caactaactc acttgaaaaa gctaaaagag gtatatacat agaatcaaat gttaacaatg
    17281 ttcctgagtt atggaagaag aagtatttta tagacaataa ggatggtaca tttaaaattt
    17341 gtgataagat tagaaaaagt gtaattttca agccatttaa tttaatgaat gatttttcat
    17401 atcaacattt tgatttgatt ttttgtagaa atgttatgat ttattttgat ttaaaaacaa
    17461 gagaagaact tataaataaa ttttataatg ctaccaaaaa agatagtttt ttgtttgtag
    17521 gacatgcaga ggtaattaac agaagtaata ctaaatataa atatgtaaaa cctgctatat
    17581 ataaaagata aaatgctagt tttgatatat gattttgtgg aggcggtaat gtgggacgtt
    17641 ttaataatat ggttattgct ataggcgcat ctgtaggtgg tacagaagca atacttgaaa
    17701 ttataaaaga cttaccaaaa tcaacaccag ggattgttgt agttcaacat atgccagcta
    17761 tatttactta tatgtatgca caacggcttg acaagcagtg tattatgaat gtaagagaag
    17821 ctaagaataa tgatagagtc gaacaaggaa atgtattaat tgcacctggg ggttgccaga
    17881 tgaaattgtg tgcggataaa caaggatatt atgtaacttg tgaaaaagga gaaagaataa
    17941 gtggtcactg tccatcagta gatgtattgt ttgattcagt agcagaagta gcaggtaaaa
    18001 attcgatagg aataatactt acaggtatgg gttatgatgg agcaaatgga cttttaaaaa
    18061 tgaaagaaaa tggttcattt acaattggac aggatgaaaa ttcttgtata gtatatggaa
    18121 tgccaatggt agcttttgat aggggttctg ttatgttaca attacctctt ggtgaaattt
    18181 caaactgttt gataagacat attaatattt taaaacaaaa agattaattt tttataaata
    18241 aaaattaatc ttttattaaa ttatatggat aaaaaaccct agaaactcca aatataataa
    18301 gagagattta tatttaggag gaaaatgata atatgaaatt ttttaaagtg gatacaaaag
    18361 ctaaagtttt aagatatgta tttgttttaa ccttgactat aatggctgtt ttggcaatta
    18421 aacatgacta taaatataat aatttgaaaa atgcttcaat taatttaaat aataaggaag
    18481 cagtgagcgt tatgagtaat cagtcattac caaagggtaa tacaaatagt aaacaaactt
    18541 tatcagtaag tacaatagga agtaaaacaa tagtgaaaaa tgatgagtat ttagaaagta
    18601 ctataaacat gcctgtaata acaaattcaa ataagattgt tgaaaggtct acaaatgata
    18661 agataaaaaa tgacatattt gaattttata ataaatcata taaagaagca aagcagtatc
    18721 ttaaggataa tcctgatgag aaaaataagt ttgttgcaaa tgtagatttt gaacttaaaa
    18781 agaatactga ttctgctctt agtataaaag tgagatatta tacttatagt ggtggagcac
    18841 atggatttta tcaagataca gcatataatg tggatatgag aactggaaaa tttttagaat
    18901 taatggactt atttaaggat aatactaaat ataaagaagt tatagatgaa gaaattaaaa
    18961 gacaaatagc tgaacttgaa aaaaaggatg aagaaaatat cggaatatat aattttaaag
    19021 gtataaaaga gaatcaaaac ttttatttgc aagatgataa tctagttata tattttgatt
    19081 tatatgatat aactccatat gcagcaggga ttccagaatt ttcaataaat aaaaaactga
    19141 taagctctat gctaaaacca gaatatgtag atttatttga tttgaaatag aagattaaat
    19201 taaatgttat gctataatat ttaacataga gacttattaa aattgggggg tataaaaatg
    19261 ctacttttaa aaaaagacga cataaaaaaa gtatttacaa tgagagatgc aattgaagct
    19321 gataaggaag cttttagaat ttattgtgaa ggaaagagtg taaatccctt gagaaccaat
    19381 ataccagttc caagtcaaga tgctagtatg ttattcatgc cagggtatgt agaagaatta
    19441 gggtgtgggg gaataaaaat tgtatctgta tttcctaaaa atgctcaaaa aggcaagcca
    19501 gtaattccag caacagtact actattagat ggtgaaactg gagaagtaag tgcagtattg
    19561 gatggtactt atgtaactca aattcgtacc ggagcagcat ctggagcagc catagatgtt
    19621 ttggctaaaa aagattctaa gattggagct ttgataggtg ttggaggaca aggtgagcct
    19681 caacttgaag ctatgataga agctagaaat ttggatgagg taagagtttt tagtagaaac
    19741 aaggaaaata gagaaaaatt tgtagaagaa atgaatttaa aattaagtaa atataatact
    19801 aaaatagttg cagttgaatc atctgatgaa gccatagata atgcagatgt aatagtttta
    19861 gctactccat caaaacaacc agtatttaat ggaaacttag taaaaaaagg agctttaata
    19921 agtgcagtag gctcatatat gccagacatg caggagttgt gtccaatttg tttaactaga
    19981 gcatctaaga tattttttga atcaacagat gcagttttat ctgagtcagg agatatattg
    20041 attccattaa aagaagggaa gataagtaaa gatgatttta gtggtgattt aggaaatgtt
    20101 ataaatggaa ctattcctgg cagagaagat gatgatgaga taattgtgtt taaaactgtc
    20161 ggaataggtg tacaagacgt cgtaacagca aaaagaatat acgacaaagc aaaagaaaac
    20221 ggtatcggaa tggactggaa atggtaaaat attcaaaata attctatagt tagataaatt
    20281 tatgttataa tgttgataaa taaatttttt attattttaa ttgagggagg atatttgtat
    20341 gaaaatggta aaaaagttaa tattatcttc tattatggct ctcgttttag ttctaactat
    20401 agggtgttct aatgcagaag aaaaagtaaa gtcaaatttt gaagctaatt tagctgcatt
    20461 agaaatgtat aagtatgatg aaaaaggaat tgctgatgca ttaacagaag ctgaatataa
    20521 acagattcaa acttataaca gcaaggataa ggtttcaatg attgctcagt tgacacctct
    20581 ttatggtaaa gaaaatgctg aaaagatata cgaaacaatt gttaagttaa ttcaaggtgt
    20641 tgaaaagact gtaacagtgg aatcttctag taaggataag ataactttga aagttgtaag
    20701 caaagcttta gatactagta aagtaatgag tgaagcgaac aatttagtaa aatcttatgt
    20761 aactgatgaa aagaattacg ataaaaaaga aaaagaagtt caagatgata tggcaaaaat
    20821 gattatagac ctttacaata agaagccgac taaggaagtt acaactatga ttacatatac
    20881 taaaaaagat ggtaaatggc aaccacctag taatatacaa aagttatgtg aaggaatata
    20941 tacaatttaa tttattttaa agacacaaaa ctatctttta agcatataat aaaatgtttg
    21001 gaagatagt
//
LOCUS       DAFNNB010000057        20858 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0057, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000057 DAFNNB010000000
VERSION     DAFNNB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0057"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1316
                     /locus_tag="L3M60_001816"
     CDS             <1..1316
                     /locus_tag="L3M60_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN6226485.1"
                     /translation="KTTGEPTRKIKNSHITLKNKEEKICSSCNKENNTKDNYCKFCGN
                     ELYEITSLRPLETKLDLKSKIKELSYHANKRGVFLTTFTTIFILFIIALIFKAIITIQ
                     FNDISYLINPAHIILALNLGQISVSMSTMMGSGFINANIGLLILLIIPILVLLISNLI
                     FMRKRCRDSKSVLANSIGVGIFYGLVLAILSIFTNVKTSSHSMLEYGYALEYSYEFFS
                     VLLNGFVLGFICTYITTYKKSYEKENMYLSLFSNSIKTFILGYVLVLIILFVLTISDS
                     SYLNELDMSSYSNGLNLFTILPQIASYMWAFANGISVTIINSTVSMFTLSSSSLFGDT
                     KLMFYAMGALSMLILLLNGYKLRFKYNTDSIKPIVVFSIYYAFLMGMLALFSTFILDS
                     NINFFNTTSYGATLIMQFKVLQAIVISFIYSFVISLIGYKLNSAD"
     gene            1338..1829
                     /locus_tag="L3M60_001817"
     CDS             1338..1829
                     /locus_tag="L3M60_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN6226486.1"
                     /translation="MEGNINNKVDDKFSKVKKKFGNGKFKVELYKEKSKLNREIDDIQ
                     EKKAKIFLEMGILTYQKIREDSIDDNSFDKFCDELLELDKLIYEKNMEINEMEMVESN
                     VTCECGYVGNVNDKYCAECGKKFELENECDDFIVCGYCESEIDTEAEFCPCCGRKIIM
                     DLE"
     gene            2105..2776
                     /locus_tag="L3M60_001818"
     CDS             2105..2776
                     /locus_tag="L3M60_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBN6226487.1"
                     /translation="MLDKVKGRLIVSCQALENEPLHSPFIMGRMAKAAMEGGAVGIRA
                     QGVEDIIEIKKVTGLPVIGIIKRNYEDSDIYITPTKKEVDELLTTGCEMIALDATNRV
                     RPNNEDLKELIKYIKENGVLVMADISNYDEAIKAQEYGVDCISTTLSGYTPYTKTLEG
                     PDFVLMERLVKDLEIPVIAEGKVNTPQDLKRVFELGVYSSVVGSAITRPQLITEKFVK
                     AIEIN"
     gene            2861..3727
                     /locus_tag="L3M60_001819"
     CDS             2861..3727
                     /locus_tag="L3M60_001819"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003452648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBN6226488.1"
                     /translation="MKGIYSALLVSFDKEGNINEKGLRQIIRHNIDVCKVDGLYVGGS
                     TGENFMLSTDEKKRIFEIAKDEVKEEIKLIAQVGSVNLKEAVELAKFTTDLGYDAISA
                     VTPFYYKFDFEEIKHYYNTIINSVDNRLIIYSIPFLTGVDMSLDQFGELFENEKIIGV
                     KFTAADFYLLERMRKTFPNKLIFAGFDEMMLPATVLGVDGAIGSTFNVNGVRARQIFE
                     LTKNEKISEALEVQHVTNDLITDILGNGLYQTIKLLLEEQGVEAGYCRQPMKEATDEM
                     KSRAKEIYRKYF"
     gene            3760..5262
                     /locus_tag="L3M60_001820"
     CDS             3760..5262
                     /locus_tag="L3M60_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HBN6226489.1"
                     /translation="MVGFTWIDFTILVVYLLAVLFAGLLFSKKEMKGKEFFKGDGTIP
                     WWVTSVSIFATLLSPISFLSLAGNSYGGTWILWFAQLGMFIAIPLTIKFFLPLYSRLE
                     IDTAYEYLEMRYESKGLRVLGALMFIIYQIGRMSIIMYLPSIVLAGLTGIPVNVLIIV
                     MGVIAIIYSYTGGLKAVLWTDFIQGMVLIVGVTGTLFYLIASINGGFGTVMDTLTTGH
                     KFLASNEVMFDKNILSTSAFIIFVGAGLNTFSSYVSSQDIVQRFTTTTDIKQLNKMTY
                     GNGVLSMGLATVFYLIGTCLFIYYTQNPDLAQTVQQDQVFASYIAYELPVGITGILLA
                     AIYAASQSTLSTGLNSVATSWTLDIQTIITKDISMEKQTKIAQYISLGVGILSIVVAI
                     VLANGEIKSAYEWFNSFMGLVLGVLAGIFVLGAFCKKATKMGAYVGFIVSAILVVYLK
                     YCMPEVTSWAYSLITITTSVVVGTIFSAIEAKVKAKVSLPRAETTVYYEK"
     gene            5392..5844
                     /locus_tag="L3M60_001821"
     CDS             5392..5844
                     /locus_tag="L3M60_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBN6226490.1"
                     /translation="MIFGNINHSKTYSTLHEDLLRCFEYAKDNSLIDYEKGTYTIDGD
                     DIFVNIVEYKTCEKEDRFWEAHKKYIDVHLMLDGCERIDINFIENLEQKIYEEEGDFL
                     PLDGKNNGYVELRKGDFLVCYPEDAHMTGIKVLEKENIKKAIFKVLVR"
     gene            6004..6807
                     /locus_tag="L3M60_001822"
     CDS             6004..6807
                     /locus_tag="L3M60_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226491.1"
                     /translation="MENIDVFLLIGQSNARGLGNPKESVIPNENCFEYLSTDEIINMR
                     CELETSEGDGTIAPAFSNEWNKLTGNKVCFIHNAKDGSRIKNWNHDNNWFLNDTIEKF
                     NAGCATLSKHYNIENKYVIWIQGESDAKYGSDALYYKESLKKIAYRLKEECMIDKMFV
                     SLTGYWLGEDEYFIRTRRIAAAQESACNECDILCVGSKIAMQFHDKGLTIDDVHYTQE
                     GLNILGKDLCKNIYKYHTTKEKELLSDTIDLSEARRYIYELEKISKKFV"
     gene            6865..7752
                     /locus_tag="L3M60_001823"
     CDS             6865..7752
                     /locus_tag="L3M60_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HBN6226492.1"
                     /translation="MKNYVCIDVGGTSIKYGFIREDGLIIDKSSLDTEAKEKGGEGIL
                     SKIKGIVKKYIKENEISGICISTAGMVDPVEGKILFALEELIPNYKGMQLKKEIEKEF
                     NIKCEVENDVNCAGLGEMWLGAGRGATSSICLTIGTGIGGCIIINNKLINGFSNSAGE
                     VGYMNINGSSFQELASTSSLIKKVAKIKNLNENELNGKIIFDLAKNNDEDCLKELDNM
                     IKSLAVGIANICYVINPEVVILGGGIMAQEKFLKPKIDKALKEVLIERVYKNTNIEFA
                     KRQNDAGMLGALYNFLNKI"
     gene            7771..8619
                     /locus_tag="L3M60_001824"
     CDS             7771..8619
                     /locus_tag="L3M60_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBN6226493.1"
                     /translation="MSILEQLESPTFKVTKSDKLLIAYIKENIDDVFYKPISQIAKES
                     NIGEATITRFVKKMNFNGLQDFKVTLAQEISTINKKNIINKNIQNDEPALDTAKKLLS
                     SNVTTLENTVEIINSKDVHDCARLIINAKKVYFIGIGYSGIIAQDSNYKFMRIGLNCV
                     SFDSSHTMIMMSSIMEEGDLIIAISHSGETEEIIKTVKLARANNAKIISITENKNSEL
                     KDISDVHLSYVSGETVLETGSISSKLAQFFIIDLVYTQVVKELSNEAIERKIKTTNAI
                     KLFKNE"
     gene            8682..9371
                     /locus_tag="L3M60_001825"
     CDS             8682..9371
                     /locus_tag="L3M60_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBN6226494.1"
                     /translation="MSKLKDYNIDKVMIFNKLSTKTKNQLKEKAKLKRLQKKEILFYE
                     KDSLDSVYMLLEGKVSIFKISENGERKVIFILNSGEVINDITFDSSKNSTVGCEAFEN
                     SIVAYYSIEEFLEIMQSDFQLTKDIISYMERRIRRLYRQLKNSISIKVDKKLAAKLYR
                     LSREFGVCCGEWTLLNVNITVTYLADMLGCKRETVSRMIKVLQKEELIKIDNKGFYVK
                     ETELSRYFKNN"
     gene            9762..10769
                     /gene="asrA"
                     /locus_tag="L3M60_001826"
     CDS             9762..10769
                     /gene="asrA"
                     /locus_tag="L3M60_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrA"
                     /protein_id="HBN6226495.1"
                     /translation="MKLKLDSSKFNEGLNYLKKDYKIFAPVSIPFKGTFSDTDVIRYK
                     EVNSFEEMEFTKKANFSPKEVVLPINQVLFYFTEKEFKESDLDNKKILIFLRACDING
                     IKRLDEIYLNNGEEKDYFYKNIREKVKFVLVGCKESFRNCFCVSMDSNKTDNYSIGLN
                     IEGDTLYLDIKDSEFEIFNGETTEFNIDYVTDNLISVDVPENIDSNEMIRNSIWDEYD
                     TRCIGCGRCNFVCPTCSCFTMQDIFYKENENVGERRRVWASCQVDGYTDIAGGNSFRK
                     KQGERMRFKTMHKIHDFKKRFGYHMCVGCGRCDDACPQYISFSKCIEKINDLVTSKEE
                     I"
     gene            10770..11561
                     /gene="asrB"
                     /locus_tag="L3M60_001827"
     CDS             10770..11561
                     /gene="asrB"
                     /locus_tag="L3M60_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfite reductase subunit AsrB"
                     /protein_id="HBN6226496.1"
                     /translation="MNPYIPVNAKILDIVKHTDKEWTFRVNCDTKGVLPGKFYEISIP
                     KYGESPISVSGYGENYIDFTIRNVGKVTSELFNYKEGDSFFIRGPYGNGFDVSLYEGR
                     EIVVVAGGSALAPVRGIVEYFYNNKEKCEKFKLIAGFKSPKDVLFADDLKRWSKKLDI
                     LVTVDGADEGYSGNIGLVTKYIPELDIKNINNTSIVVVGPPMMMKFTVGEFLKRDLDE
                     KNIWVSYERKMCCGIGKCGHCKMDNTYICLDGPVFDYSYAKNLID"
     gene            11574..12545
                     /gene="asrC"
                     /locus_tag="L3M60_001828"
     CDS             11574..12545
                     /gene="asrC"
                     /locus_tag="L3M60_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit C"
                     /protein_id="HBN6226497.1"
                     /translation="MIRDLNTKKVMKNAYRITKTKYETSLRVRVPGGLIDPESLTIVS
                     KIASEYGNGQVHITTRQGFEILGINMEDMEEINKIIQPVIEKMNINQSEKNAGYPAAG
                     TRNIAACIGNKVCPKAQYNTTKFAKRIEKAIFPNDLHFKVALTGCPNDCIKARMNDFG
                     IIGMALPIYEKDRCVNCGACVKKCSKISVGALKTENNKVVRDGDKCIGCGECVLNCPT
                     NAWTRDEKKYYRLAIMGRTGKKNPRLAEDFLLWVDENSIIKIILNTYKYVEKYIDKDA
                     PGGKEHIGYIIDRTGFMEFKKWALEGVELPEITKMYENIYWSGVKYL"
     gene            12602..13378
                     /locus_tag="L3M60_001829"
     CDS             12602..13378
                     /locus_tag="L3M60_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBN6226498.1"
                     /translation="MHKETLDKLTNAAINKINLLNTSKVKYLVSSAFAGLYVGIGILL
                     IFTIGGLLTDAGSPMTKIVMGLSFAIALSLVIMTGTELFTGNNMVMSAGMLNKGVSIK
                     DTSKIWAYSWVGNLIGALILGLIFVGTGLVDKGPVAEFFANTAASKASMPFTALFFRG
                     VLCNILVCVSVLCSFRTNSDTAKIIMIFLCLFAFITSGFEHSIANMTIYSVSLFSPTI
                     STVTIGGAIYNLVAVTLGNIVGGALFMGLGTYILGKEKLN"
     gene            13650..15668
                     /locus_tag="L3M60_001830"
     CDS             13650..15668
                     /locus_tag="L3M60_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBN6226499.1"
                     /translation="MTKKLVISKTKLFIFIIAFISIFIYLFGSKNTLIGVGIVTAMLT
                     LLERDLTISPIKNLLKYLAINIILGILSFFAVQNMYLGVLLNFIALFIIGYIFSYDLK
                     RAVYVPFGLMYIFMVSIPVGISEFPMRLSALAAGAVIIMIAQFVMNRNRMKNIGDKEL
                     ISICDELLEKISLLKNIINDDSSISKSSMRKIDSCNYRINSISKNLKMVIFDNRKDDF
                     YISIRGIDIMNILFSLERINLILERYKKDSKEFEDEDIKNILEESSINSKSDVLVVAT
                     KEVNYIKMCLENKDTITDKEILGFRDYVIAQDTKNINLKEIYSVLENLYEFLVDYKKI
                     KSRDEKKAERKSNIPHEFKRLSIYKKNFNLNSIRFSYAVKIALATAVAGFIMDYFHLR
                     DGRWIMLTVFSLTQPYAENCIQRSRKRIEGTFIGAVIFIVLFSIIKDSTLRSLIVLLA
                     GYINSYVVDYRKLMVCVTVSALGSAVVMGDPNVLTISRISYVALGAIIALIVNKFILP
                     YDAKTGYQHVIDMYKGIVKNIIDEVNKSIENVADVYYIKNLLLVPSLIEDRIMLINAI
                     YKDKYQEDFLENQKLLISNMYNLYINVNKNKIQDEDVEKILRDTNYISNYNVDKYDEG
                     RSVILESIVNTKTLGDKIICLNLLQTLNGVKEMYRISNITKVSIKEVA"
     gene            15883..16740
                     /locus_tag="L3M60_001831"
     CDS             15883..16740
                     /locus_tag="L3M60_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6226500.1"
                     /translation="MSKDVKVKRNKKYDTFSITMELERPLLYDEVENKDDKKNNRKFN
                     IKNKIFKGIGILFVLILLSFFIWINKTYEPQKLAKEALISNSKVEVTVKENISFTPKG
                     RKVSKGLILYPGAKVEIESYAPLARKIAESGYEVVIVKMPLNLAILGTNKAQKVMDSY
                     NNIEHWVIGGHSLGGVVASNFARDNKLIDGVVFLASYPMGDELKELGKKVISIWGSKD
                     GVVNFKSLVESKQKLPDDTTYVEIEGGNHAQFGDYGKQKGDNDAIISQEKQLNITANS
                     IIKFLKNLS"
     gene            16836..17705
                     /locus_tag="L3M60_001832"
     CDS             16836..17705
                     /locus_tag="L3M60_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6226501.1"
                     /translation="MEYDMPLYRPPSEAYSLIIQVTLGCSHNKCTFCSMYKSKKFKIK
                     PLEVIKNEIDIFRRYYKHVERIFLADGDALIIPMEKLRDIILYIKEVFPECERITMYG
                     SPKSIEKKSDDELIELRKLGVKMIYLGLESGSNEVLEDIKKGFNSEQLIKVGRKVKKS
                     GIKLSATVIAGLGGTKKTHQHAVDTGKMLGKISPDYVGVLSLMVEPNTELYDLLKAGE
                     FTILEDKAVLQEIKEMIKNIDANEKIVFRSNHASNYANLKGVLPDDKQRLIDEIDYYL
                     SNQKLKREEYRRL"
     gene            complement(18078..18710)
                     /locus_tag="L3M60_001833"
     CDS             complement(18078..18710)
                     /locus_tag="L3M60_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBN6226502.1"
                     /translation="MTIPNSTKVYPRNNDYQTVYLKNVITRENIEVGDYTIYNDFCND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHTLKSKSTYTFPIFYEEW
                     GLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNNTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDELV"
     gene            19471..20238
                     /locus_tag="L3M60_001834"
     CDS             19471..20238
                     /locus_tag="L3M60_001834"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBN6226503.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKEDIIKEAKELKGVCLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYISDIPLLFTFRSLAEGGEKVISKDYYTTLN
                     KEISSTGLVDLIDVELFMGDEVIDEVINFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQDEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELSSVLNLLHKSIN"
     gene            complement(20579..>20858)
                     /locus_tag="L3M60_001835"
     CDS             complement(20579..>20858)
                     /locus_tag="L3M60_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226504.1"
                     /translation="DIKLDKESIDKIYNRRKIKIEREVNIFEDEDVIINKKVLKFPKS
                     WTKNMQKYWLSENKYPIHSTVIDDDRYKCCNVQYTKNRVIILYYIYNL"
BASE COUNT         8344 a         1955 c         3554 g         7005 t
ORIGIN      
        1 ccaaaacgac aggtgaacca actagaaaaa taaaaaatag ccatatcact cttaaaaata
       61 aagaagaaaa aatttgttct agttgcaata aagaaaataa tactaaggat aattactgta
      121 aattttgtgg aaatgaactt tatgaaataa catcattaag accacttgaa acaaaactag
      181 acttaaaatc taaaataaaa gagctttcat atcatgcaaa taaaagagga gtatttttaa
      241 caacatttac aaccatattt atattattta ttattgcatt gatatttaaa gctattataa
      301 ctattcaatt taatgatatt agttatttaa taaatccagc acatataatc ctagctctca
      361 acttaggtca aattagtgta tctatgtcta ccatgatggg ttctgggttt ataaatgcca
      421 atataggttt gttaatatta cttataattc ctattttggt attattaata tcaaacttaa
      481 tttttatgag aaaaagatgt agagattcaa aatctgtatt agcaaattct ataggtgttg
      541 gaatttttta tggattagtt ttagctattt tatctatttt tactaatgta aaaactagtt
      601 cacatagtat gttagaatat ggatatgcat tagaatatag ttatgaattt tttagtgtcc
      661 ttttaaatgg atttgtatta gggtttatat gtacatacat aactacttat aaaaaaagtt
      721 atgaaaagga aaatatgtat ttatctctct tttcaaattc tataaaaaca tttatattag
      781 gttatgtttt agtattaata atactatttg tactaactat atctgatagt agttacttaa
      841 atgaactaga tatgagtagt tactcaaatg gattaaattt atttactata ctgccacaaa
      901 tagctagcta tatgtgggct tttgcaaatg gaatttcagt aaccataata aattcaacag
      961 tatcaatgtt tactttgagt tcttcatctt tatttggaga tactaaatta atgttttatg
     1021 caatgggagc attatctatg ttaatattat tattaaatgg atacaagcta agatttaagt
     1081 ataatacgga tagtataaaa cctatagtag tatttagtat atattatgct tttttaatgg
     1141 ggatgttggc tttatttagt acatttatac tagatagtaa tattaatttc tttaatacaa
     1201 ctagttatgg agcaacttta ataatgcaat ttaaagtctt acaagctatt gtaatttcat
     1261 ttatatatag ttttgtgata tctttaatag gttataagct aaactcagct gattaaatat
     1321 gattggaggg aacatctgtg gaaggaaata ttaataataa agtagatgat aaattttcta
     1381 aggttaagaa gaagtttggt aatggaaaat ttaaagttga attatataaa gaaaaatcaa
     1441 agttaaatag agagattgat gatatccaag aaaagaaagc aaagatattc ttggaaatgg
     1501 gaatattgac atatcaaaaa attagagaag attctattga tgataatagt tttgataaat
     1561 tctgtgatga gttactagaa cttgataagt taatttatga aaaaaatatg gaaattaatg
     1621 aaatggaaat ggtagaaagt aatgtaactt gtgaatgtgg ctatgttgga aatgtaaatg
     1681 ataaatattg tgctgaatgt ggcaaaaaat ttgaattgga aaatgaatgt gatgatttta
     1741 tagtttgtgg ttattgtgaa agtgagatag atacagaggc tgaattttgt ccatgttgtg
     1801 gaagaaaaat tataatggat ttagaataat attgggaaac ctatgtatag tatacatggg
     1861 tttttttatt gaaattattt atatagaata catataatag tggttataca aaatgaaaat
     1921 aaacaccaaa aaaatgaaat aaaatttctt ttaagtataa aaaacagact aatatataga
     1981 ataaatcagt aaaaatatag agttaagaaa aaaatatgaa aaaatattgc aaagaaaaag
     2041 ttttcatgtt attattaata cataaatcaa gaaaataaat ttcagaaatg aggtaattga
     2101 aaaaatgcta gataaggtaa agggaagatt aatagtatca tgccaagctt tagaaaatga
     2161 acctcttcat agtcctttta taatggggag aatggctaag gctgctatgg aaggtggagc
     2221 tgttggaatt agggctcaag gagttgaaga tattattgag ataaaaaaag ttactggact
     2281 tcctgttata ggtattataa agagaaatta tgaagattca gatatatata taacaccaac
     2341 taaaaaagaa gttgatgaac ttttgacaac agggtgtgaa atgatagctt tagatgctac
     2401 taatagagtt agaccaaata atgaagactt aaaagaactt ataaagtata taaaagaaaa
     2461 tggtgttctg gtaatggcag atatttcaaa ttatgatgag gctatcaaag cacaagaata
     2521 tggtgtagat tgtatttcaa ctacactatc tggatatact ccatatacta aaacgttaga
     2581 aggtccagat tttgtattaa tggaaagact agttaaagat ttagaaatac ctgtaatagc
     2641 agaaggaaag gtaaatactc ctcaagattt aaaaagggtt tttgaactag gagtctattc
     2701 atcagttgta ggttcagcaa taacaagacc acaattaata acagaaaaat ttgttaaagc
     2761 aattgaaatt aattaaaaat taatttataa attaaaaaac tattatattt aactatactt
     2821 gaaaattaaa attggaggta aagatatgag aacaacagat atgaagggaa tttattcagc
     2881 attattagtt tcatttgata aggaaggaaa tataaatgaa aaagggttaa gacaaataat
     2941 tagacataat atagatgttt gtaaagtaga tggtctttat gtaggaggaa gtacagggga
     3001 aaactttatg ctttcaactg atgaaaagaa aagaatattt gaaatagcta aagatgaagt
     3061 gaaggaagaa ataaaattaa tagctcaagt tggttcagtc aatttaaaag aagctgtaga
     3121 gcttgctaaa tttacaactg atttaggata tgatgcaata agtgctgtta caccttttta
     3181 ctataagttt gattttgaag aaataaaaca ctattataat acaataataa attctgtaga
     3241 taatagatta ataatatact ctataccatt tttaactgga gttgatatga gtttagacca
     3301 gtttggagaa ttatttgaaa atgaaaaaat aataggtgtt aagtttacag cagcagattt
     3361 ctacttatta gagagaatga gaaaaacatt cccaaataaa ttaatatttg caggatttga
     3421 tgaaatgatg ttaccagcta cagttttagg tgtagatggg gcaattggtt ctacatttaa
     3481 tgtaaacggt gtcagagcta gacaaatatt tgaattaact aagaatgaaa aaatatcaga
     3541 agcacttgaa gtacaacatg tgactaatga tttaataaca gatatattag gaaatggatt
     3601 atatcaaaca attaaattgc tacttgaaga acaaggtgtt gaggctggat actgtagaca
     3661 acctatgaaa gaagcaactg atgaaatgaa atcaagagca aaagaaatat acagaaagta
     3721 tttttaataa aatactttta gtatgggagg attagtcaaa tggtaggatt tacttggata
     3781 gattttacaa tactagtggt ttacttattg gcagttttat ttgcaggatt gttattttca
     3841 aaaaaggaaa tgaagggaaa agagttcttc aaaggtgatg gaactattcc gtggtgggtt
     3901 acatcagtat caatatttgc cacattatta agtccaatat cgtttttatc attagcagga
     3961 aattcttatg gtgggacttg gattttgtgg tttgcacaat taggtatgtt tatagctata
     4021 cctttaacaa taaaattttt cttacctcta tatagtaggt tagaaattga tacagcttat
     4081 gagtacttag aaatgaggta cgaaagcaaa ggtcttagag tgcttggagc tttaatgttt
     4141 ataatatatc aaataggacg tatgtctatt ataatgtatc ttccatcaat agttctggct
     4201 ggtcttacag gaatacctgt aaatgtactg ataatagtta tgggtgtaat agcaataatt
     4261 tactcatata caggtggatt aaaagctgtt ttatggaccg attttataca aggaatggta
     4321 ctaatagtag gtgttacagg aacattattc tatttaatag caagtataaa tggtggtttt
     4381 ggaacagtaa tggatactct tacaactgga cacaagttct tagcaagtaa tgaagttatg
     4441 tttgataaaa atatactatc tacaagtgca tttataatat ttgttggagc tggacttaat
     4501 actttttcat catatgtatc gagtcaagat atagttcaaa ggtttactac aacaactgat
     4561 ataaagcaac ttaataaaat gacatatgga aatggtgttt tatcaatggg acttgcaaca
     4621 gtattttatt taattggaac ttgtttattt atatactata ctcaaaatcc agatttagct
     4681 caaacagttc aacaagacca agtatttgca tcatatatag cttatgagtt accagtaggt
     4741 ataacaggaa tactacttgc agcaatatat gcagcttctc aatcaacttt atcaacaggg
     4801 cttaactcag ttgctacaag ttggacttta gatatacaaa ctatcataac aaaagatata
     4861 agtatggaaa aacaaactaa gatagcacaa tatatatcat taggtgtagg tatactttct
     4921 atagttgttg caatagtatt agcaaatgga gaaataaagt cagcatatga atggttcaat
     4981 agttttatgg gtctagtact aggtgtctta gcaggaatat ttgtacttgg tgcattttgt
     5041 aaaaaagcaa ctaagatggg agcatatgtt gggtttatag tatcagcaat tttggttgtt
     5101 tatttaaaat actgtatgcc agaagtaact tcttgggcat attctttaat aacaataact
     5161 acttctgtag tggtaggtac aatatttagt gcaatagaag ctaaagttaa agcaaaagta
     5221 tctttaccaa gagctgagac tactgtttat tatgaaaaat aatatttaaa tgataaattg
     5281 tcttatagag tgattagata aataatttag ttactctata agattgtata atatacttta
     5341 tatataaaag tataaaaata gactttataa ttatataggg agatagtgct tatgatattt
     5401 ggaaatataa atcatagtaa gacatattca acattgcatg aagatttact tagatgtttt
     5461 gaatatgcta aagataattc attaatagat tatgaaaaag gtacttatac aatagatggt
     5521 gatgatattt ttgtgaatat tgtagagtat aaaacttgtg aaaaagaaga tagattctgg
     5581 gaagctcata agaagtatat agatgttcat ttgatgcttg atggatgtga acgaatagat
     5641 ataaatttta tagaaaattt agaacaaaaa atatatgaag aagagggaga ttttttacct
     5701 cttgatggta aaaataatgg atatgtagaa cttagaaaag gcgatttttt agtttgttat
     5761 ccagaagatg ctcatatgac aggaataaaa gttttagaaa aagaaaatat caaaaaagct
     5821 atttttaagg ttttggttag ataattataa tttatataac cactagggct gaaaatagtg
     5881 atttaacttt cagcgattag tgagacttcg taaattttag ttagattact attttcagcc
     5941 ttagtttttt ataaggaaat tgaaaataat taaaagttta aaattttgta aaggagttaa
     6001 cttatggaaa atatagatgt attcttatta ataggacaaa gcaatgctag aggattaggg
     6061 aatcccaaag agagtgtcat tccaaatgaa aattgttttg aatatttaag tactgatgaa
     6121 attataaata tgagatgtga gctagaaact tcagaaggtg atggtactat agcacctgca
     6181 ttttcaaatg aatggaataa gcttactgga aataaagttt gttttattca caatgcaaaa
     6241 gatggttcaa gaataaaaaa ctggaatcat gataataact ggttcctaaa tgacactata
     6301 gaaaaattta atgcaggctg tgctacctta agtaaacatt ataatataga aaataaatat
     6361 gttatttgga ttcaaggtga aagtgatgcg aaatatggta gtgatgcatt gtactataaa
     6421 gaaagtctaa aaaagattgc ttatagactt aaagaagaat gtatgattga taaaatgttt
     6481 gtaagtttaa ctggttattg gcttggagaa gatgaatatt ttattagaac aagacgaatt
     6541 gcagcagcac aggagtctgc ttgtaatgaa tgtgatatat tatgtgtagg aagcaaaatt
     6601 gctatgcaat ttcatgataa agggttaact attgatgatg tacattacac tcaagaaggt
     6661 cttaatatat taggaaaaga cttgtgtaaa aatatttata aatatcatac aactaaggaa
     6721 aaagaattat taagcgatac aatagattta tcagaagcaa gaaggtatat atatgaattg
     6781 gaaaagataa gtaaaaaatt tgtttaatat aagagtttaa gtagtgttat caatttataa
     6841 aaataattta tatggagata tgttatgaaa aattacgttt gtatagatgt tggaggtact
     6901 tcaataaaat atggatttat tagagaagat ggattaataa ttgataaatc aagtcttgat
     6961 actgaagcaa aagaaaaagg tggagaagga atattatcta aaataaaagg tatagtaaaa
     7021 aagtatataa aagaaaatga aataagtggt atatgcatat caactgcagg tatggttgac
     7081 ccagttgaag gtaaaatact atttgcatta gaagaattaa ttccaaacta taagggtatg
     7141 caattaaaaa aagaaataga aaaagagttt aatataaaat gtgaagttga aaatgatgtt
     7201 aattgtgctg gacttggtga aatgtggctt ggagcaggta gaggtgcaac atcttctata
     7261 tgtttaacaa taggtactgg aattggtgga tgtattataa taaacaataa actgataaat
     7321 gggtttagta atagtgctgg cgaagtagga tatatgaata taaatggaag ttcttttcaa
     7381 gaattggcat ctactagcag tctaattaaa aaagtagcaa aaataaaaaa tttgaatgaa
     7441 aatgaattaa atggaaaaat aatttttgat ttagcaaaga ataatgacga agattgttta
     7501 aaagaactcg ataatatgat aaaatcatta gcagttggaa tcgcaaatat atgttatgta
     7561 ataaatccag aggtagtaat tttaggtggt gggattatgg cacaagaaaa gttcctaaaa
     7621 ccaaaaatag acaaagcatt aaaggaggtt cttatagaga gagtttacaa aaacactaat
     7681 atagaatttg caaagagaca aaatgatgcg ggaatgcttg gggcattata taatttttta
     7741 aacaaaatat aaagaataga agggttttta atgagtattt tagaacaatt ggaatcacca
     7801 acttttaaag tcacaaagtc agataaatta ttaatagctt atataaaaga aaatatagat
     7861 gatgtgtttt ataaaccaat atcacaaata gctaaagaaa gcaatattgg tgaagcaacc
     7921 ataactagat ttgtaaaaaa aatgaatttt aatggattac aggattttaa agttacttta
     7981 gcacaagaaa tatcaactat caataaaaaa aatattataa ataaaaatat acaaaatgat
     8041 gagccagctt tagatacagc aaaaaaactt ttaagttcaa atgtaaccac tttagagaac
     8101 acagtggaga taatcaatag taaggatgta catgactgtg caaggcttat aataaatgca
     8161 aagaaagttt acttcatagg tattggatat tctggtataa tagcacagga ttcaaactat
     8221 aaatttatga gaataggtct taattgtgta agttttgaca gtagtcatac catgataatg
     8281 atgtcttcca taatggaaga aggagattta ataattgcaa tttcacattc tggtgaaaca
     8341 gaggagatta taaaaactgt aaagcttgca agagccaata atgctaaaat tatatcaatt
     8401 actgaaaata aaaattctga attaaaggat atttcagatg tacacttatc ttatgtatca
     8461 ggagaaacag tactggaaac cggttcaata tcttctaaat tagcacaatt cttcattatt
     8521 gatttagtat atacacaagt agtaaaagaa ctttctaatg aagctattga aagaaaaatt
     8581 aaaactacaa atgctataaa attgtttaaa aatgaataaa ataagaaatt tgtaatgttt
     8641 tatagagctc tcttaaaaac ttaggaatgg agagatatta catgtctaaa ttaaaagatt
     8701 ataatattga taaagtaatg atttttaata agttaagtac caaaacgaag aatcaactta
     8761 aagaaaaagc taaactaaaa agattacaaa aaaaagaaat tttattttat gaaaaagata
     8821 gcttagacag tgtttatatg cttttagaag gaaaggtatc tatttttaag ataagtgaaa
     8881 atggtgagag aaaagtaatt ttcatattaa atagtggaga agtcataaat gatattacat
     8941 ttgactcttc taaaaactcc actgttggct gtgaagcatt tgaaaactca attgtagctt
     9001 attatagtat tgaagagttt ttagaaatta tgcaaagtga ttttcaactt acaaaagata
     9061 ttatttctta tatggaaaga agaataagac gtttatatag acagttgaaa aattctatat
     9121 ctataaaagt agataaaaaa ctcgcagcaa aattatatag gttaagtaga gaatttggag
     9181 tttgctgtgg agaatggact ttactaaatg taaatattac agttacatat ttagctgata
     9241 tgcttggctg taaaagagag actgtttcta gaatgataaa ggtattacaa aaagaagaac
     9301 ttattaagat tgataataaa ggtttttacg ttaaagaaac tgaattatca aggtatttta
     9361 aaaataatta gttatgatta aataagggta ttattaggat tttaattaat taaaaatatt
     9421 attgggagaa tctatttata gattcttttt tattgtctaa tttattaatc cttattttac
     9481 ctttcctaaa ttgtataaaa aagcaaataa gactctctat tattcaaaaa gttgagtaga
     9541 taatagattt attgtattta atgattgtat ttaagtgact agatttgtaa tcgaataata
     9601 tagataataa aaaaatatta atagagctta ataaactcta ttaatatttt tttattattt
     9661 ttgaaactgt gataattatc acagaagcat agaaaataaa atgttaagat ttaaacaaga
     9721 taagaagtaa gtaaaactaa aatataagaa aggtgataag aatgaaatta aaattagatt
     9781 catctaaatt caatgaaggt ctgaattatc taaaaaaaga ctacaagata tttgcaccag
     9841 tttcaatacc atttaaaggt actttttcag atactgatgt tataagatac aaggaggtaa
     9901 actcttttga agaaatggaa tttaccaaga aagcaaattt ctcaccaaaa gaagttgtac
     9961 ttcctataaa tcaagtatta ttttatttta ctgaaaaaga gtttaaggaa agcgatttag
    10021 acaacaagaa aattttaatt ttcttgagag cttgtgacat aaatgggata aaaagacttg
    10081 atgaaatata tctaaataat ggcgaagaaa aagattattt ttataaaaat atcagagaga
    10141 aagtaaagtt tgtacttgta gggtgtaagg aaagttttag aaactgtttt tgtgtgagta
    10201 tggattctaa caaaactgat aattattcaa ttggattaaa tatagaagga gatacactgt
    10261 atttagacat aaaggatagt gaatttgaaa tatttaatgg agaaacgact gaatttaata
    10321 ttgattatgt aactgataat ttaatatctg tagatgtgcc agaaaatatt gattcaaatg
    10381 aaatgataag aaattctata tgggatgaat atgatacaag atgtatagga tgtggtagat
    10441 gtaattttgt atgtccaaca tgttcatgtt ttacaatgca ggatattttc tataaggaaa
    10501 atgagaatgt aggtgagaga agaagagttt gggcttcttg tcaagtagat ggatatacag
    10561 atatagcagg tggaaattct tttagaaaaa aacaaggtga aagaatgaga tttaaaacta
    10621 tgcataaaat acatgatttt aaaaaaagat tcggatatca tatgtgtgtt ggttgtggaa
    10681 gatgtgatga tgcctgtcca caatacatat cattttcaaa atgtatagaa aaaataaatg
    10741 atttagtaac tagtaaggag gaaatataaa tgaacccata tataccagta aacgcaaaaa
    10801 tattagatat agttaagcat actgataaag aatggacttt tagagtaaat tgcgatacaa
    10861 aaggtgtatt gcctggaaaa ttttatgaga tatctatacc taaatacggt gaaagcccaa
    10921 tatcagtatc aggatatgga gagaactata ttgactttac aataagaaat gtaggaaaag
    10981 taactagtga gttgtttaat tataaagaag gagattcttt ttttataaga gggccttatg
    11041 gaaatggatt tgatgtatct ctttacgaag gaagagaaat tgtagtagtg gcaggaggaa
    11101 gtgcattagc ccctgtaaga ggaatagttg aatattttta caacaataaa gaaaaatgcg
    11161 aaaagtttaa attaatagca ggatttaaat caccaaaaga tgttttattt gcagatgatt
    11221 tgaagagatg gagtaaaaaa cttgatattt tagttactgt agatggagca gacgaaggat
    11281 atagtggaaa tataggatta gtgacaaaat acataccaga attggatata aaaaatataa
    11341 ataatacttc tatagttgta gttggacctc caatgatgat gaagtttaca gtaggagaat
    11401 ttttaaaaag agatttagat gaaaagaata tttgggtttc ttatgaaaga aaaatgtgtt
    11461 gtggtatagg taaatgtgga cactgtaaaa tggataacac ttatatatgt ttagatggac
    11521 cagtatttga ttattcatat gctaaaaatc ttattgatta gggggcgaag tttgtgataa
    11581 gagatttaaa taccaaaaaa gtaatgaaaa atgcttatag gattacaaaa acaaaatatg
    11641 agacttcttt aagggttaga gtacctggtg ggcttattga ccctgagagt ttaactatag
    11701 tttctaaaat tgcaagtgag tatggtaatg gacaggtaca cataactaca agacagggct
    11761 ttgaaatatt aggaataaat atggaagata tggaagaaat aaataaaatt atacaacctg
    11821 ttattgaaaa aatgaatata aatcaaagtg agaaaaatgc aggttatcca gcagcgggta
    11881 caagaaatat agcagcatgt ataggaaata aagtctgtcc aaaggctcaa tataatacaa
    11941 ctaaatttgc taaaagaata gaaaaagcta tatttccaaa tgatttacat tttaaagtgg
    12001 ctttaactgg ctgtccaaat gattgtataa aagctagaat gaatgatttt ggaattattg
    12061 gtatggcttt acccatatat gaaaaagata gatgtgtaaa ttgtggagcg tgtgtaaaaa
    12121 aatgttctaa aatatctgtt ggagcattaa aaacagaaaa taataaggtg gtaagagatg
    12181 gagataaatg tataggatgt ggagaatgtg tactaaattg cccaacaaat gcatggacta
    12241 gagatgagaa aaaatactat agattagcta taatgggtag aacaggaaag aaaaacccta
    12301 gactagcaga agattttctt ttatgggtag atgagaatag cataataaag ataatactta
    12361 atacttacaa atatgtagaa aagtatatag ataaagatgc accaggagga aaagaacata
    12421 taggatatat aattgataga acaggattta tggagtttaa aaaatgggcg cttgaaggtg
    12481 tagaacttcc agaaataact aaaatgtatg aaaacatata ctggagtgga gttaaatatc
    12541 tataaattga gtaaaacata tatttgaata aattaaaatt taatagaagg aagagggaat
    12601 tatgcataaa gaaactttgg ataaattaac taatgcagct ataaataaga taaatttatt
    12661 aaacacaagt aaagtaaaat atttggtatc ttctgcgttt gcaggacttt atgtaggaat
    12721 aggtatactt ttgatattta ctattggagg acttttgact gatgcaggtt cacctatgac
    12781 taagatagta atggggttat cctttgcaat agcactaagt ttagttataa tgacaggaac
    12841 agagttattt actggaaaca atatggttat gtctgctggt atgttaaata aaggtgtaag
    12901 tataaaagat acttctaaaa tatgggcata cagttgggta ggtaatttaa ttggagcttt
    12961 aattttaggt cttatatttg taggaactgg tctagtagat aaaggacctg tagctgaatt
    13021 ttttgcaaat acagcagcta gtaaagcatc catgccattt acagctcttt tctttagagg
    13081 ggttttatgt aatattctgg tatgtgtatc agtattgtgt tcgtttagaa ctaatagtga
    13141 tactgcaaaa ataattatga tattcttatg tttatttgcc tttataacat caggttttga
    13201 gcatagtata gcaaatatga caatttatag tgtttcatta ttttcaccaa caataagtac
    13261 agttactatt ggaggagcta tttataattt agtagctgtg acacttggaa atattgttgg
    13321 aggagcttta tttatgggtc ttggaactta tatattgggt aaagaaaagc ttaattaaat
    13381 ttattgtata aattataact ctcttaataa atacagtttt agtattgttg agagagttat
    13441 ttttatattt ttgaaaaaat atttgaaaga aaaaatatat gggagcatac gaaaatatat
    13501 aaactatata tgcaataaaa tacgtttaaa tatagattat atagttcaaa atgtaaaaaa
    13561 agttaaagtc ctaccgtaaa aaagagtatt ttgaataaga taacaaatat gataaaattg
    13621 gtcttgttga aatttttagg ggggatataa tgacaaagaa actagtaata tcaaaaacaa
    13681 aattgtttat ttttataata gcatttattt ctatattcat atatttattt ggaagtaaaa
    13741 acacacttat aggtgttggt atagtaacag ctatgctaac actattggaa agagatttaa
    13801 ctatttctcc aattaaaaat ttactaaagt atttagcaat aaatataatc ctaggaatac
    13861 tatcattttt tgcagtacaa aatatgtatt taggcgtttt attaaatttt atagcattat
    13921 ttataatagg gtatatattt agttatgact taaaaagagc agtatatgta ccttttggcc
    13981 taatgtatat atttatggta agtatacctg taggaataag tgaatttcct atgagattgt
    14041 cggctttagc tgctggagca gttataataa tgattgctca atttgttatg aacagaaata
    14101 ggatgaaaaa tattggagat aaagaactga tatctatatg tgatgaatta ttagaaaaaa
    14161 ttagtctttt aaaaaatatt attaatgatg atagttctat aagtaaatct agtatgagaa
    14221 aaatagatag ctgtaattat agaataaatt ctatatctaa aaatttgaaa atggttattt
    14281 ttgataatag aaaagatgac ttttatataa gtattagagg tatagacata atgaatattt
    14341 tattttcatt agaaagaata aatttaatcc tagaaagata caaaaaggat agtaaagaat
    14401 ttgaagatga agatataaaa aatattttag aagaaagtag cataaatagt aaatcagatg
    14461 ttctagttgt agcaacaaaa gaagtaaact atattaagat gtgtttagaa aataaagata
    14521 ccataactga taaagagata ttaggattta gagattatgt tattgctcag gatactaaaa
    14581 atataaattt aaaagaaatt tatagtgttt tagaaaattt atatgagttt ttagtagatt
    14641 ataaaaaaat aaaatcaaga gacgaaaaga aagctgaaag aaaatcaaat atacctcatg
    14701 aatttaaacg actatcaata tataagaaaa actttaattt aaattctata agattttctt
    14761 atgcagtaaa gattgcttta gctacagctg tagcgggttt tattatggat tatttccatt
    14821 taagggatgg aagatggata atgttaactg ttttttcatt aacacaacct tacgcagaaa
    14881 attgtataca aaggtctaga aaaagaatag aaggtacatt tataggagct gtaatattta
    14941 ttgtattatt ttcaataata aaagatagta cattaaggtc gttaatagtt cttttagcag
    15001 gatatataaa ctcttatgta gttgattata gaaaattaat ggtatgtgta actgtttcag
    15061 ctcttggttc agctgtagtt atgggagacc caaatgtttt aacaataagc aggatttcat
    15121 atgtagcatt aggagcaatt attgcattaa tagtaaataa gtttatatta ccatatgatg
    15181 ctaaaacagg ctatcaacat gtgatagaca tgtataaagg aatcgttaaa aatataattg
    15241 atgaagtcaa taagtctata gaaaatgtag ctgatgttta ctatataaag aatttattat
    15301 tggtaccatc acttattgaa gatagaatta tgttgataaa tgctatatat aaagataaat
    15361 atcaagaaga ttttttagaa aatcagaaat tactaataag taatatgtat aatctatata
    15421 taaatgttaa taaaaataaa atacaagatg aagatgttga aaaaatattg agagatacta
    15481 attatatttc aaattacaat gtagataagt atgatgaggg aagaagcgta attttagaga
    15541 gtattgtaaa tactaaaact ctaggagaca agataatatg tctaaactta ctgcaaactc
    15601 taaatggtgt caaagaaatg tatagaataa gtaatataac taaagtttca ataaaagaag
    15661 tagcttagat attaaattat agaatagcca tatatttaaa ataaagtata tggctatttg
    15721 ttttataaaa tattaaaaga aaataatata taaaaaacaa aaaacatata tttatgaatt
    15781 ttatatgaaa atagttaaaa tattgatttt tttcattggt tgaaaaaaca atttacttag
    15841 gataaactat aatagtattc aaatattatg ggggttagat ttatgagtaa agatgtaaag
    15901 gtaaaacgca ataagaaata tgatacattt agtataacta tggaactcga aagaccttta
    15961 ctttatgatg aggttgaaaa caaagatgat aaaaagaata atagaaaatt taacataaag
    16021 aataagatat ttaagggaat aggtatacta tttgtattga ttttgcttag ttttttcata
    16081 tggataaata aaacatatga acctcaaaaa ctggcaaaag aagctctaat cagcaatagt
    16141 aaagttgaag taactgtaaa agaaaacatt tcatttacac caaaaggaag aaaagtttct
    16201 aaaggtttaa ttttgtatcc aggagctaaa gtagaaatag aatcttatgc accacttgca
    16261 agaaaaatag cagaaagtgg atatgaggtt gtaattgtaa agatgccact taatctagct
    16321 atacttggaa ctaataaggc gcagaaagtt atggattctt acaacaacat agaacattgg
    16381 gtaataggag gtcattcatt aggaggagtt gtagcatcaa attttgcacg tgataataaa
    16441 ttaattgatg gagttgtatt tttagcttct tatccaatgg gagatgagtt gaaagaatta
    16501 ggaaaaaaag ttatatctat atgggggagc aaagatggtg tagtcaattt taaaagtctt
    16561 gtagaatcaa aacaaaaact tcctgatgat acaacttatg ttgaaattga aggaggaaat
    16621 catgcacaat ttggagacta tggaaaacaa aagggagata atgatgctat tataagtcaa
    16681 gaaaaacaac ttaatattac tgccaatagt atcattaaat ttttaaagaa tttatcttaa
    16741 actaaaatta aaaaatataa ttttataagt tattatttaa agtaaatatt aataatataa
    16801 tagaataaat attttattac aagggggatt ttaatatgga atacgatatg ccactttaca
    16861 gaccgccaag tgaggcttat agtcttataa tacaagtcac actaggatgt tctcacaaca
    16921 aatgtacgtt ttgtagtatg tacaaatcta aaaaatttaa aataaagcca ctagaagtaa
    16981 taaaaaatga aattgatata tttagaagat attataaaca tgtagaaaga atattcttag
    17041 cagatggaga tgcattgata ataccaatgg agaaattaag agacataatt ttatatataa
    17101 aagaagtttt tccagagtgt gaaagaatta ctatgtatgg ttctcctaaa tctatagaga
    17161 aaaaatctga tgatgagctt atagagttaa gaaaacttgg tgttaaaatg atttatttag
    17221 ggcttgaaag tggaagcaat gaagtattgg aagatattaa gaaaggtttt aatagtgaac
    17281 aattaataaa agttggtaga aaagttaaaa aaagtgggat taaattatca gccactgtaa
    17341 tagcaggtct tggaggaact aaaaaaacac atcagcatgc cgttgataca ggaaaaatgt
    17401 taggaaaaat ttcaccagat tatgtaggtg tacttagttt aatggtagaa cctaatactg
    17461 aactttacga tttacttaag gcaggagagt ttacaatact agaggataag gctgtgttac
    17521 aagaaattaa agagatgata aagaatatag atgccaatga aaaaattgta tttagaagta
    17581 atcatgcatc aaattatgct aatttaaaag gtgtattacc tgatgacaaa caacgtctaa
    17641 ttgatgaaat tgattactat ctaagtaacc aaaagttaaa aagagaagaa tatagaagac
    17701 tgtagttttt gatatatcaa tctatattta tagataaata ctgagattgt ttagatacaa
    17761 aaaatcatgc tatattaata gttttttact attaatatag catgattttt ttgttaaaaa
    17821 acaaaatggt atgttcatat ataggctatc aataatgatg aaaatgtgaa ctggtttaat
    17881 agaattttac atattataat ggacccatga atttaaataa taaaaagtaa agtaacttta
    17941 tattaagcaa ctttactttt tatttttgtt tagtattgta ttaaatttat atatattaaa
    18001 atgatataca aatcttgaaa aaattcttat aaaaaggatt attaaagtaa aatttaatat
    18061 atataaatta taaaagatta aactaattca tctatatttc ctgattgaat atgcttaatg
    18121 tttgattgga tttttttata tggccaatcc caccatttaa tttccaataa ttttgaaatt
    18181 gtattattat taaatctttt ttttataatc tttgcaggta ctcctccaac aatagaatat
    18241 ggtgggacat cattagtaac aactgctctt gttccaataa ttgcaccatc gccaatatgt
    18301 acacctgaca taattataga atcatagcct atccacacat catttccaat tataatatta
    18361 cctttattgt cccaagcatc cgttatatga ttgatatcga gtccccattc ttcataaaag
    18421 ataggaaagg tatatgtgga ttttgatttc agtgtgtgat ttccactagt cattagaaat
    18481 tttgcattgc atgctattga gcaaaactta ccaattatta atttatcatt gttaataggg
    18541 taatgatata atacgttgtt tttctcaaaa tctcttgggt cattacaaaa gtcattatat
    18601 attgtgtaat ctccaacttc tatattttct cttgttatta catttttaag atatacagtt
    18661 tgataatcat tatttcttgg atatacctta gttgaatttg gaatagtcat atttaaatct
    18721 ctcctttgat atatattatg tttttgacta ttccactatt acaatacatc tcttcacaga
    18781 atcactcctt gatagtatct atattttaat ttatacattt tgtttttaaa aacagacttt
    18841 taactattta gtattttata aaggctatat gcttagttga ttgtatatca aaacaataaa
    18901 gcttgcaata atttaatata ttttatatac aaaataattt ttaatttaaa gaacattaac
    18961 attgttatcc ttttaatcta cactgtcact taatgctaaa ggatgattat atggtactat
    19021 tacaatcaat tactcttgaa gtatagctgt aaaatctatt ttgggtttag tatggtgttt
    19081 aactttagcc aaaactctga agtaatgcaa atgatttgaa tttattgata tcaaatttct
    19141 tcataagtag taaaattcga gttatttcta tataattata ctataattta tatattaatt
    19201 ctattatcat ccagaataat attgattata tagataaaac ttaagtaatg ttatgaaaat
    19261 tatagaatgt taaaataaat ttagataaga aagaagtgtt tttatattat agagtatctt
    19321 ttctaaaata tgtgggattg ttttaagaat tagaataaaa attattttta aattagtatt
    19381 tttataatgt gtagcattta taatgtataa ttaaattatg cagaaaatag aatttaaaat
    19441 catatagaat ttctgattat ggaggagaat atgaaaagaa aagttcaagt aaaaaatatt
    19501 actattggag aaggtagacc taaaatttgt gtaccgatta tcggtaaaaa taaagaagat
    19561 attataaaag aggctaaaga attaaaagga gtatgtttgg atattataga gtggcgagtt
    19621 gatttttttg agaatgtaga aaatattaaa gaagttaagg aagttctcta tgaattaaga
    19681 agttatatat ctgatatccc tttattattc acatttagaa gtttagcaga agggggagaa
    19741 aaagtaatat caaaagatta ttatactact ttaaataaag aaatttctag tacaggttta
    19801 gtagatttaa tagatgtaga gttatttatg ggagatgagg ttatagatga agtaattaat
    19861 tttgctcata aaaaagaagt gaaagtaatt atatctaatc atgactttaa taaaactcca
    19921 aaaaaagaag aaattgtatc tcgtttatgt agaatgcagg aattaggcgc agatttaccc
    19981 aaaatagctg ttatgccaca agatgaaaaa gatgtgttag ttctacttga agctacaaat
    20041 gaaatgttta aaatttatgc agatagacca ataataacaa tgtcaatgtc gggaatggga
    20101 gttataagca gattgtgtgg ggaaatattt ggttctgcat taacttttgg agctgcaaaa
    20161 agtgtttcag caccaggtca aatatcattt aaagagttaa gctcagtact taatttatta
    20221 cataagagta ttaattaatt taatataatt atatagaaat attaggagtt atttttgaag
    20281 atatataaat taaatataaa aaacttttca gatagtaaaa tttaaattgg ttaattaagt
    20341 gtaatatctg aaaagcaact atttagtgtg tattttgaaa gtggtataaa aatcttataa
    20401 atcaactatg gcatatttaa aatatgccat taaaacctac tagacattgg attatagatg
    20461 gtatactagc atttctatta tttatagata gtaagttttt ataataactg ttttatctaa
    20521 agtagaagat atatttgtat tatgtgtatt tgtaattact tttttgtaat tatgtatatt
    20581 aaaggttata tatgtaatat aatattatca ctctattctt tgtatattgt acattacaac
    20641 atttatatct atcatcatca attacagtag aatgtatggg atacttattt tcacttagcc
    20701 aatatttttg catattcttt gtccaagatt ttggaaattt caatactttt ttgtttatta
    20761 taacatcctc atcttcaaaa atattaactt ctctttctat ttttattttt cttctgttgt
    20821 atattttatc gatagattct ttgtcgagtt ttatgtct
//
LOCUS       DAFNNB010000058        20849 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0058, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000058 DAFNNB010000000
VERSION     DAFNNB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20849
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0058"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..368
                     /locus_tag="L3M60_001836"
     CDS             <1..368
                     /locus_tag="L3M60_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6226505.1"
                     /translation="ANRILGFVNAPSLFQRVIARSLDAEVDVNIYKKNFDLLYNSLID
                     MGYSCVKPSGTFYLFPKAPIEDDKKFCNDAKQFNLLLVPGSSFGCPGHFRVSYCVSYD
                     KVKSSLPAFEKLAKLYNLK"
     gene            538..1500
                     /locus_tag="L3M60_001837"
     CDS             538..1500
                     /locus_tag="L3M60_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBN6226506.1"
                     /translation="MKKGYVYIILTTLFFSSMEISLKTVTNDFNPLQITLSRFFVGGL
                     VLLPFAVKRLRALNLSITKSDLKYFAFLGFMCVVVSMSLYQLAVLNTKASVVAVLFSC
                     NPVFVMLLAYLILKEKIYRHNVISLILEVLGIIVIINPLHTKLTLSGIFLTLSSAIIF
                     SMYTVFGKRKTLKFGGIVVTCFSFIFGSLEMLILVLLTKINFIADILSKNKLNVFANI
                     PIFSGYTLHNMPIMIYVFVFVTGVGYALYFMAMEATSTSLTSLVFFFKPVISPILALL
                     ILKEIIPINMIIGILLIVIGSIISIIPTIITQMHNKHVEALYKE"
     gene            complement(1622..2284)
                     /gene="zmp1"
                     /locus_tag="L3M60_001838"
     CDS             complement(1622..2284)
                     /gene="zmp1"
                     /locus_tag="L3M60_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease Zmp1"
                     /protein_id="HBN6226507.1"
                     /translation="MRPSKKLLIAIISIFLISSVPVSAHAESTTIQQNKDILSQIVVF
                     PTGNYDKNEANAMVNRLANIDGKYLNVLNQNNLKIKLLSGKLTDEKEYAYLKGVVPKG
                     WEGTGKTWDDVPGLGGSTVALRIGFSNKGKGHDAINLELHETAHAIDHIVLNDISKSA
                     QFKQIFAKEGRSLGNVNYLGVYPEEFFAEAFAYYYLNQDTNSKLKSACPQTYSFLQNL
                     AK"
     regulatory      complement(2384..2469)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(2654..5557)
                     /locus_tag="L3M60_001839"
     CDS             complement(2654..5557)
                     /locus_tag="L3M60_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021362860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HBN6226508.1"
                     /translation="MRKGNKKALLISLIMILSMVMSTIYPTVSYALELGENSQIQSGS
                     TNSSTGEEKESDNKKPEQTPEEEKQTDNKKPEQMPEKEKPTDNKNPEQTPEKEKPTDN
                     KNPEQTPVGEKPIDSKNPEHTQEDKSTDNKKSEQSLEDEKTLDNKNPEKTPKEEKLLE
                     EENLLKVLEDELNEENEDYGFIVKINNNTIETESMKKISFNLTYTPTSKGIQAGDSIT
                     FKVPDVFNKVNLDYTSECFDKTESNGEYTLTFRELPNGQSIMQGKIGLEAYVKKVDED
                     TNAKIHIETTGKIENGSGDVDVEIKPGDKTEVPDAKGTLKKLVEGRKSTTVFMPVKNK
                     DINYSIQVNEKQEELKDIILYDELPEGLTLINGSVSVVTSDGKEVSDFNITQSKNSIS
                     VNFGNIDKSYTVKYKARISDKNAKHGNKYKNVARIESDGKKIQEDDATVSVFDRGDNY
                     LLTKGHSGATNITQVGQVINYQISVNDDKSPISNVVITDNIPEGMRLTTSGEAGHDFR
                     VVEVPMNGSWTPWSKEKIANNISYKVEEKRNESGQVDKVITGFTINLSKEEVESKFFI
                     AYTLKVISIEDSYINRAVLDANNSEIDKNDEINFKKNSGLISAKKEVDKKVLSSSDNQ
                     IVKYKINMSTYGVYDAGQVNLLDEVNPALKISNIKYSDNLELKKEAGDGKNTIRLVNK
                     YEFKQIKEGEPVQSWVTFDADFTNVKVGETIQNIAQINGSSPPGVETTKQGYAFEAKK
                     VDALDKNVLSGARFNLEDVSGNIVVKDLVSDEDGVIQSSVKKPGIYYLVEFRPPNGYE
                     KLKDKIKVEIGNKDIGRIVDIGNIENIRQENPPVPPNTDEPVVNPPVPPSTDKPKKPS
                     SPSDTDETVVINPPVPPSEDMTNPPIPEVVNPPIPPSEEVIETPVKQIVPIPEVVKPS
                     VSEEKNNKVKDDTLVNPPVPPKTGDNTTIIGEILLVLGAIVGLIVLKRNKNAN"
     regulatory      complement(5670..5755)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01786"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01786"
     gene            complement(5882..7060)
                     /locus_tag="L3M60_001840"
     CDS             complement(5882..7060)
                     /locus_tag="L3M60_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HBN6226509.1"
                     /translation="MFSKILVKTFIRDSENVQNRDVRNKYGYVAGVVGILSNLLLFVI
                     KVFIGMLTSSIAIMADAFNNLSDMASSAITMIGFKLASKPADKEHPFGHGRIEYLSAL
                     IVAFMVMLVGLQFVKSSIERIVNPVPIKFEVIPLILLIASIMIKIWLSRFNKFMGNKI
                     DSSALKAVSLDALGDVFTSSCVVISFIVARFTNFPIDGYVGIVVSLVILYAGFSLVKD
                     TINPLLGEAPDEDMVNSIIELLLSYKHIIGTHDLIIHNYGVGRCIASIHAEIPSNIDI
                     MEIHEIIDTAEREISEKLDIYLVIHMDPVCLEDKEVMSAKKELEEILKKNSLVKSMHD
                     FRIVGKGSKKNLIFDIVVNPSEFSKNMSEDDLKEDITKLVKEINPEYDCVIVVDKDFI
                     "
     gene            complement(7751..10525)
                     /locus_tag="L3M60_001841"
     CDS             complement(7751..10525)
                     /locus_tag="L3M60_001841"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBN6226510.1"
                     /translation="MRYYNKPTKEVLKYLKTNPEIGLDDNEVEERKLRYGLNEFTIKE
                     GRTFWDELGESLTEPMILILIGAAVISSFVGELHDALGILGAIFIGISIGIITEGKSK
                     KAAQALSKLTENIEVKVLRNGKIIKISKNDLVPGDIVYIETGDMIPADGRLIQSINLK
                     LREDMLTGESDDVAKNADAVLDMEVVYSKTEIIEQDAIPAKQVNMVFGGTLVAYGRGI
                     MVVTHTGDKTEMGKIAQNLSNEDQQTPLQIKLGKLGAKIAGISGVIATLLCMFMIIQM
                     QRKGMLILDTSSVLSFLQSLEPAKNAYMVCIALIVATVPEGLPTMINITLAITMQKMA
                     KINALVTKKEACETIGSVSVICSDKTGTLTQNKMMVEVAYVDGKYISGGEYQSNSYFE
                     QNCIVNSTADIEKEDNSFKYIGSATECALLLYHNDKNYNEMRKQTYLISQIPFSSEEK
                     KMSTLIRQEDSDILLSKGAPEVLLKKCSYVQQGKNIVPITSKIEKSILDEIKKLQIKS
                     MRTLGFAYKKMSNSKTEVAITSEGELNLIGSSRSYMKEDNLVFSGFVGIIDPLREGVK
                     ESIDKAFNAGVDVKMLTGDNINTATAIGNELGLLNDGKKAVEATYIDVLTDKELREEI
                     KGISIVARSKPDTKMRIVSALQKSGEVVAVTGDGINDAPALSQADVGIAMGISGTEVS
                     KNAADIILTDDSFSTIVEGIKWGRGIYENFQRFIQFQLTVNIVAFIIAIISQLTGKDM
                     PFTTIQLLWVNIIMDGPPALALGLEPVRDYVLKRKPINRHSGIIARSMFVNIIINAIL
                     IITIVFAQSAFNILGATSEEQGTVIFSLFAFSALFNALNCREFGLNSTIPNFFKNKLA
                     LQIIVVTGIIQIIFTQVFQSFFNSVSLDFDMWIKIILFASTILLSNEFVKLILRMMKN
                     SRTMNCNSKN"
     gene            complement(10696..11952)
                     /locus_tag="L3M60_001842"
     CDS             complement(10696..11952)
                     /locus_tag="L3M60_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBN6226511.1"
                     /translation="MLKTTKISDPELYKIVANELVRQEHNIEMIASESTAPTEVLELS
                     GCVFTNKTEEGYPGARFQAGSEEADKLETLAIKRAKEVFGAEHVNVQPYSGSTANYCV
                     YSSILKPNDTVLSMRLDQGGHLTHGSTVNFLHDIYKYEFYGVDPNTGRIDYDALEAKA
                     KECKPKLIIAGASSYPRLIDYERISKVAKEVGAYFMVDMAHVAGLVAAKVIPSPVPYA
                     DFVSSSTTKTFCGPRSGIILCKAEHAKKLDKGVFPGTLGSIHLNTVAAKAFSLLYLGT
                     DKFKKIMEQVVVNAQTLASELISHGFSIVSGGTDNHIVMVDLRSKNLTGKQFEKALEY
                     VGITVNKNVIPDDPQSPFVTSGVRIGLTSISQRGLKEKEVIQIAGIMNKVAENIDNKE
                     VLDECKAEAQELISKFPLYPEGYFED"
     gene            complement(12024..13526)
                     /locus_tag="L3M60_001843"
     CDS             complement(12024..13526)
                     /locus_tag="L3M60_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBN6226512.1"
                     /translation="MRALNVIERAGNKLPDPVTIFLLLCIIVVILSAIISNLGVEEIH
                     PSTKEVVKVVNLLEKEQIQSYLGSIVTNFQSFAPLGLVLVTMLGAGVAEKSGFMEVLM
                     KKGISKVPQKLVTVAIVFAGMLSHTAADVGFIILPPLAALVFLGIGRHPLVGMFAAFA
                     GVAGGFAANVMLSTTDVLLAGFTIPAAQMMDSSYQGNPAMNLYFAFVSAIILTFAGAF
                     VTEKFIAPRFSKYSDAVNDKDMEELSELENKGIKYALLSLLAIVVIIVALCIGENAFM
                     KDPETGSILSSNAPLMKGIVPIITIIFLTPGLVYGRVSNKIKNDKELVSMMGSSMSDM
                     GGYIVLAFIASQFINLFNLSNLGTILSITGAKVLAESGIPSYGLIIGFILLSGFINLF
                     VGSASAKWAILAPIFVPMFMLLDFNPALTQIAYRIGDASTNPISPLFPYFPVILAFAR
                     RYDKDIGIGTVISNMIPYSVVFTLIEVIILLLFMSIGIPLGPGGGISYVL"
     gene            complement(13625..14809)
                     /locus_tag="L3M60_001844"
     CDS             complement(13625..14809)
                     /locus_tag="L3M60_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226513.1"
                     /translation="MHELISISEKYKEDILDFFTDIHMHPELSFKEFRTTKVIKDLLV
                     SLDIEILDLGMETGVVGLLKGKYDGPTVALRGDIDALPICEEVDIEYKSRYDGIMHAC
                     GHDIHTSCLVGCAYVLSHIRDSLHGNVKFIFQPAEEVNKGAKMLVERGVMENPKVDAI
                     FGLHNHPDIPCGKIGVKLGGLMAAVDTIKIEVNGFGGHGGIPNRTIDPIVASSAIIMG
                     IQTIVSRNISPLESAVISIGTINGGTANNVISEKVDMTGTCRSFSNEVRKKISENLEK
                     IVCEIARGYQATAKLDYLFDLPAVINSKEMYTIACKSVCDLYSEDAIVDPVPSTGGED
                     FSIFMEKAPGFFYWLGVGNKEQDCMYQWHNPKFKADKNSILVGTNVLCQSVINYMDKL
                     KK"
     gene            complement(15040..16128)
                     /locus_tag="L3M60_001845"
     CDS             complement(15040..16128)
                     /locus_tag="L3M60_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226514.1"
                     /translation="MSDKVNIKNIICIAGAYLATAIGSGFATGQEILQFFASQGYMGL
                     VGALISMVLFSFLGAEVITRGRELKLKEPTRIYTFYCGKILGKFYEWFSPIFLFGILI
                     VMISGAGSTLTEYYGINPYLGRVLMAVATLITVSMGLEKLVSILGRIGPVIAVFTIAV
                     GLISLFKHFDGLSMVGEAMKNIEVTKASESPIISGVIYNTLNIIVMIVFLTGMGASIE
                     SKKDAFWGGLLGGIVFMTAGIVMYLAMISDIGNLYMKEIPSLYLADNIAPIVGVCFSV
                     ALILGIYTTAVPLLWSVTNRIVEDEHPKFKLVTTVIAILACIGGFLPFGKLVNILYPY
                     TGYMGLLILVCMVYKAITCREKSTQGQE"
     gene            complement(16412..17110)
                     /locus_tag="L3M60_001846"
     CDS             complement(16412..17110)
                     /locus_tag="L3M60_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HBN6226515.1"
                     /translation="MRRALIIDDEEAVLKIITHFIEMKDMPIKIVGKATSGDEAVDKI
                     TGLEPDIVFIGIQMPIYNGLEVIEKTSHLSKKINFVVITAFDYFEYAQKALRLNVKDI
                     LLKPLDMKEFTKSVEKIIGYQYTNNDLLNEILEYINLNYSNDLKLNDCARLFLTNPSN
                     ISRIFKSNLNTTFISYLNHIRIEKSIELLENTTKSINEIAEIVGYNSLNNFYKNFKIK
                     KGMTPKVYKLNTKN"
     gene            complement(17224..18729)
                     /locus_tag="L3M60_001847"
     CDS             complement(17224..18729)
                     /locus_tag="L3M60_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBN6226516.1"
                     /translation="MKIQNTEWGYIEWKHTYDENNPKQAMNIYIAVTMPGKKHFNHVH
                     YGQEQMIYILEGEGLYIINGVWKPFYQGMIFYIESGSTHETINTGDREIKELVVSNIV
                     NNFETSEILEFNKNNKIENTPVNYSENTLNLYAAVESIRGQFIDPFKIPLIIYDDYWN
                     IVLKNPYFPLFCFEKCNPMKFPQNCDCMNQKSANQFTCKYGITIYNISIIYKNNSIGV
                     IRGGYILMSDSSLGVGHRNLYDIPEGAARTMKRLLNQIAKNIINFCSFNDIRKDLQEK
                     EKTIARTYHYGEQLEMNLKVAQDMVTNLRINHHFLFNTLNSMVSIALDDGSYDLYSAI
                     IDLSRMFRYTMRSDLRFVELESEILYIENYLNLQKLRYGDALKVKYLIPKKLYNLSVP
                     FNFIQPIVENAFTHGSRDIDTEKRIEIIARLDSQYAIIEIHNNGTILDKNNIDKIKAG
                     IRINNGHGLSLIYTKFTSAYGNNFDMDIKSSDNEGTYIMIKIPSENYKEYV"
     gene            complement(19002..20273)
                     /gene="serS"
                     /locus_tag="L3M60_001848"
     CDS             complement(19002..20273)
                     /gene="serS"
                     /locus_tag="L3M60_001848"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBN6226517.1"
                     /translation="MLDIKRIRENLDCIKKAMERRGEKAFNLDEVVKLDDERRKILQE
                     VEVMKNKLNTASKNIPNLIKEGKDVENEKIKLKDLSDKIKVIDQNLKEVEDKMEYLLM
                     RIPNVPHPGVPQGETDDDNVEVRSWGETTKFDFESKAHWEIGTELGILDFETASKITG
                     SRFTLYKGLGARLERALLNFYLDTNTKVNGYTEVIPPFMANRNSFLGTGQLPKFEEDM
                     FKIEGLDYFMIPTSEVPLTNIHANEILKFEQLPINYTAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVEMVKIVAPEESYNELEKLTNNAETMLQLLNLPYRVVKICTGDLGFTASFK
                     YDVEVWMPSYNRYVEISSCSNCEDFQARRAGIRFKRDKDSKAEYAHTLNGSGLAIGRS
                     VAAILENYQQEDGSVIVPEVLRPYMGVSVIK"
BASE COUNT         6589 a         3600 c         2366 g         8294 t
ORIGIN      
        1 ttgcaaatag gatacttgga tttgtaaatg ctccatctct tttccaaaga gttattgcaa
       61 gaagtttaga tgctgaagtt gatgtaaata tttataaaaa gaattttgac cttctatata
      121 atagtttaat cgatatggga tattcttgtg ttaaacctag tggtacattc tatttattcc
      181 caaaagctcc aattgaagat gataaaaaat tctgcaatga tgcaaaacaa tttaatttat
      241 tattagtacc aggttcttct tttggttgtc caggtcattt tagagtatca tattgtgtat
      301 cttatgacaa agttaaaagc tctttaccag catttgaaaa attagccaaa ttatataatt
      361 taaaataatt aaatcaaagt aattcaaata attaactacc ttagtataga atacttacat
      421 tctatattaa ggtagtttat aattttatgt actatatttt atacatacca tattattcca
      481 atatacttga atattcttat ctatagtact aatataaatt acaaggaggt gtttgacttg
      541 aaaaaaggtt atgtttatat aatactgact accttatttt ttagttcaat ggaaattagt
      601 ctaaaaactg tcactaatga ctttaatccc ttacaaataa ctttatcaag attttttgtt
      661 ggcggtttag tcttactacc ttttgctgta aaaagattaa gagctcttaa tctttctatt
      721 acaaaatcag acttaaaata ctttgcattt ttaggtttta tgtgtgtagt agtaagtatg
      781 tcattgtatc aactggctgt tctaaacaca aaggcatctg tagttgctgt tttgttcagt
      841 tgtaatccgg tctttgtaat gcttttagct tacttaattt tgaaggaaaa aatatatagg
      901 cacaatgtaa tttcacttat attagaagtt cttggaatta tagttataat aaatccattg
      961 catacaaaac taactcttag tggaatattc ttaacattat cttctgctat tattttttca
     1021 atgtatactg tatttggtaa aagaaaaacc ttaaaatttg gtggaatagt tgtaacttgc
     1081 tttagcttta tatttggtag tttggaaatg ctgatattgg ttttactcac taaaattaat
     1141 tttatcgcag atatattaag taaaaataaa cttaatgtat ttgcaaatat acctattttt
     1201 agtggatata ccttacacaa tatgccaatt atgatttatg tattcgtatt tgttacaggt
     1261 gttggttatg cactttactt tatggcaatg gaagctacat ctactagtct tacatcttta
     1321 gtcttcttct ttaaaccagt aatatctcca atactagcat tattaatact taaagaaatt
     1381 atccctataa acatgattat aggaatttta ttgatagtaa ttggttcaat aatttctatt
     1441 atacctacga taattactca aatgcataat aaacatgttg aagccttgta taaggagtag
     1501 taattgatac tctatcagtt tatacataag acaaaattta ttagttttaa acgcaatata
     1561 aaagagatgc tattacaata gcatctctta atttatattt attttaaatt tattaaatta
     1621 tctatttagc taggttttgt aaaaaactat aagtttgtgg acaagctgat tttaacttac
     1681 tatttgtatc ttgatttaga tagtaatacg caaatgcttc tgcaaaaaat tcttctggat
     1741 atacacctaa gtagtttaca ttccctaaag aacgtccttc tttagcaaat atttgtttaa
     1801 attgtgctga tttagatata tcatttaata ctatatggtc tattgcatgt gctgtttcat
     1861 gtaattctaa gtttattgca tcatgacctt tacctttgtt actaaatcct attctaagag
     1921 ctactgtgct tccacctaaa cccggaacat catcccaagt ttttccagtt ccttcccatc
     1981 ccttaggaac tacacctttt aaataagcat attctttttc atctgttagc tttccactta
     2041 ataattttat ttttaagtta ttttgattta atacatttaa atactttcca tctatattag
     2101 ctagtctatt taccatagca ttagcttcat ttttatcgta atttccagtt ggaaaaacta
     2161 ctatctgact aagtatatct ttattttgtt gtatagtagt actctctgca tgtgcactta
     2221 ctggaactga acttattaag aatattgata ttatagctat taataatttt ttacttggtc
     2281 tcatattatt ccctcctttg agttaggccg ggagtaataa atttcaaaat aaaaaagata
     2341 cccaagagta tctttgtata tacaaaaaga aatttttact cttttcggca actggctggc
     2401 atggtttact aaactttagc ccctatagct ttgcgtgact aggtttccct agttttgcct
     2461 catttaattt tatatctata ttatagctta tatcttttta attgtctatg ctctttagaa
     2521 tttttttcta aaaagttatt atttttttac ataaatcttc aaaaaaaaat atcttctaac
     2581 aatattttca ctattaaata ttattttaga agatattttt taatattact tacatttttt
     2641 ataatgttag ggtctaattt gcatttttat ttctttttaa tacgataagt cctacaatag
     2701 ctcctagaac taaaagtatt tctccaataa ttgtagtatt atcccctgtc tttggtggta
     2761 ctggtggatt taccaaagta tcatctttaa ctttattatt tttttcttct gatactgatg
     2821 gctttactac ttctggtatt ggaacaatct gtttaacagg agtttctatc acctcttctg
     2881 atggtggtat tggtggatta actacctctg gtattggtgg atttgtcata tcttctgatg
     2941 gtggtactgg tggatttata actacagttt catctgtatc agatggactt gatggttttt
     3001 ttggtttatc tgtgcttggt ggcactggtg gatttaccac tggttcatct gtatttggcg
     3061 gtactggtgg attttcttgt cttatgtttt ctatatttcc aatatccact attctaccaa
     3121 tatctttatt accaatttca accttaattt tatccttcaa tttttcatat ccatttggag
     3181 gcctaaattc caccaaataa tatattcctg gtttttttac actagattga attacaccat
     3241 cttcatcact aactaaatcc ttaactacta tatttccaga aacatcttct aaattgaacc
     3301 tagcacctga aagtacattt ttatctaaag catccacttt tttagcttca aaagcataac
     3361 cttgcttagt agtctctact cctggagggc ttgaaccatt aatctgagct atattttgta
     3421 tagtctcccc aacttttaca tttgtgaagt ctgcatcaaa tgtaacccaa ctttgcacag
     3481 gttcaccttc ttttatctgt ttaaattcgt atttatttac taatcttatt gtattttttc
     3541 catctccagc ttctttttta agttcaaggt tatcagaata ttttatatta gatattttta
     3601 gagctggatt tacttcatct aaaagattta cttgccctgc atcataaacc ccataagttg
     3661 acatattgat tttgtattta acaatctgat tatcactact acttaaaacc tttttatcaa
     3721 cttctttttt tgcgcttatt aatccagaat ttttcttaaa gtttatctca tcatttttat
     3781 caatttcact attatttgca tcaagaacag ctctatttat atagctatct tcaatactaa
     3841 ttacctttaa tgtataagct ataaaaaact ttgattctac ttcttcttta ctaagattaa
     3901 ttgtaaatcc agttataact ttatcgactt gcccactttc atttcttttt tcttctactt
     3961 tatatgaaat attgttggca attttttctt tactccaagg agtccagcta ccattcattg
     4021 gaacttctac aactctaaaa tcatgtccag cttctcctga agtagtcaat ctcatacctt
     4081 ctggtatatt atctgttata acaacattac tgattggtga cttatcatca tttactgaaa
     4141 tctgatagtt tattacttga ccaacttgag taatatttgt tgcccctgaa tgtcctttag
     4201 tgagaagata attatcccct ctatcaaaca cacttacagt agcatcatct tcttgtatct
     4261 tcttcccatc agattcaatc ctagctacat tcttatattt attgccatgt tttgcatttt
     4321 tgtcactgat tctagcctta tatttaacag tatacgattt atctatattt ccaaaattaa
     4381 ctgaaataga gtttttactc tgtgtaatgt taaaatcact aacttctttt ccatcacttg
     4441 ttactacact tacagagcca tttatcagtg tcaatccttc aggaagctca tcatacaaaa
     4501 taatatcttt taactcttcc tgcttttcat ttacttgtat actgtaattt atatctttat
     4561 ttttaactgg cataaataca gtagttgatt ttcttccttc aacaagtttc ttaagtgttc
     4621 ctttagcatc tggaacctcc gttttgtctc caggttttat ttctacatct acatctccac
     4681 ttccattttc tatttttcca gtggtttcaa tatgtatttt tgcattagta tcttcatcaa
     4741 cttttttaac atatgcttcc aaacctattt tcccttgcat tatactttgg ccattaggta
     4801 attctctaaa tgtaagtgta tactctccat tactttctgt tttatcgaaa cattcacttg
     4861 tataatctaa attaacttta ttgaatacat ctggaacctt aaaagttatt gaatctcctg
     4921 cttgtattcc ttttgaagtt ggagtataag ttaaattaaa tgatattttt ttcattgatt
     4981 ccgtttctat tgtattgtta ttaattttta caataaatcc atagtcttca ttttcttcat
     5041 taagttcatc ttctaaaact ttcaataaat tttcttcttc taatagtttc tcttcctttg
     5101 gagttttttc tggattttta ttatctagtg ttttttcatc ttctaaagat tgttctgact
     5161 ttttattatc tgttgactta tcttcttgtg tatgctctgg atttttacta tctattggct
     5221 tttctcctac tggagtttgc tctggatttt tattatctgt tggtttctct ttctctggcg
     5281 tctgttctgg atttttatta tctgttggtt tctctttctc tggcatctgt tctggttttt
     5341 tgttatcagt ttgcttttct tcctctggag tttgttctgg ttttttattg tcgctttcct
     5401 tttcttctcc agtggatgaa ttagttgaac cactttgaat ctgactattc tctcctaatt
     5461 ctaaagcata agatacagtt ggatatattg tagacatcac catacttaaa atcataatta
     5521 aactaattaa taatgccttt ttatttcctt tcctcatata aatttctccc ctctaggtta
     5581 tctatacttt ctaatcttcc attaatatca tatataaatt aatttttact aaaaaattat
     5641 acaaaaaaag ctatgaaaat aaataaattt atattcatag ccggttgcac cactgactca
     5701 gtatatccat aaggtgccca ataatagaat atacttcaac atctctataa tatttcataa
     5761 aaacaattaa aattgtttca tttatattaa aaatacaatt atatacttct attatattat
     5821 gcctttatac tattgtctat cttttttaca ttttttacct tattatgaga ttaattaata
     5881 attaaataaa atccttatct acaacaatta cacaatcata ttcaggattt atctccttaa
     5941 ctaattttgt tatatcttct tttaagtcat cctccgacat gtttttggaa aattcggaag
     6001 ggttaactac tatatcaaat attaaattct tcttgctccc ctttccaact attctaaaat
     6061 catgcataga ttttacaagg ctatttttct taagtatttc ttcaagttct tttttggctg
     6121 acataacttc tttatcttca agacatactg ggtccatgtg aattactaga tatatatcta
     6181 atttttcaga tatttctctc tctgcagtat ctataatttc atgtatttcc attatatcta
     6241 tgttggatgg aatttctgca tgtatagatg caatacatct accgactccg tagttatgaa
     6301 taattaaatc atgtgtacct attatatgtt tataagataa caatagctct ataatagaat
     6361 ttaccatatc ttcatctggt gcttctccta gaagaggatt tatagtatct tttacaagag
     6421 agaaaccagc ataaagtatc actaatgata ctacaatacc aacatatcca tctataggaa
     6481 aattagtaaa tctagctaca ataaacgata taactacaca acttgaagta aatacatctc
     6541 ctaaagcatc aagtgaaact gcttttaatg ctgaagaatc aattttattt cccataaatt
     6601 tattaaaacg gctcaaccag attttaatca ttatagatgc aattaaaagt ataagtggta
     6661 ttacttcaaa ttttatagga actggattta ctatcctttc tatagatgac tttacaaatt
     6721 gaagtccaac caacatcacc ataaaagcta ctattaatgc tgataaatac tctattcttc
     6781 catgaccaaa aggatgctct ttatcagctg gcttacttgc taatttaaaa cctatcatag
     6841 ttattgctga agatgccata tcagaaagat tattaaatgc atcagccatt atagctatac
     6901 tggatgtgag cattcctatg aatactttta tcacaaataa aagtaaattg gataatatgc
     6961 ctactacacc agccacataa ccatatttat ttctaacatc tctattttga acgttttcgc
     7021 tatctctaat aaatgttttg actaggattt tggaaaacat gaaacttacc tccttgtcct
     7081 ttattttatt aacaaatttt agaatctata tctttattta tgataacata atttatatat
     7141 aaaattctta aaaaattact atacttcaat aaaatattat tatatatcaa tttaaataca
     7201 aatcattata tttagcaaag tttttatgcc ttaatatctg cttaatacat tctatgtcat
     7261 tttttaaaaa gattatttat tacgataata tcatttttcc caagtattcc ttagaatatg
     7321 atttttttat tttgtctata catattttaa aattatactg tatttatcta tattttataa
     7381 ccttatgata cactataact ataagatttt aaacggaaag gaatgaaata aatcatgttt
     7441 tttatcagtg atatttacac aaaatcaccc attaaatttg atacttcatt gcaaaaagag
     7501 gtatataaaa tattacaaaa gttaaatatt gacttttaac atattgacac agatgaagca
     7561 attataatgg aagattgtgt ttagattaat aaaaaattaa atatgaaaat taaaacaaaa
     7621 caaatcattc atgatttctt atcttttgca ggtcatacac catctataat tgaattgtaa
     7681 tataaaatag cataaaaaaa acacaaaccg caaagtcaca atatattggt actttgcggt
     7741 ttcataagaa ttaattttta ctattacagt tcatagttct actatttttc atcattctga
     7801 gtattagctt tacaaattca tttgacaata ggatagttga tgcaaatagt attattttta
     7861 tccacatatc aaaatccaaa gaaacagaat taaagaaact ctggaacact tgtgtaaata
     7921 ttatttgaat tatccctgta actactatta tttgcaatgc cagtttattt ttaaagaaat
     7981 ttggtattgt actattcaaa ccaaactctc tacaatttaa tgcattaaaa agggcactga
     8041 atgcaaataa agaaaatata acagtacctt gttcttctga tgtagctccc aatatattga
     8101 aagctgattg agcaaataca attgtaatta taagaatagc atttataata atatttacaa
     8161 acatagacct agctattatt ccagaatgtc tatttattgg ctttcttttc aatacataat
     8221 ctctaacagg ttctaatcct aatgctaagg ctggaggacc atccattatt atatttaccc
     8281 ataataattg tattgtagta aatggcatat ccttaccagt aagttgagat attatagcaa
     8341 ttataaatgc tactatattt acagtaagct gaaactgtat aaatctttga aagttctcat
     8401 atatacctct tccccatttt attccctcta ctatagtact aaaactatca tcagtcaata
     8461 ttatatctgc tgcattttta gatacttctg ttcctgaaat acccattgca attccaacat
     8521 ctgcttgact aagagctggg gcatcattta taccatcacc tgttacagct acaacttcgc
     8581 cacttttttg aagtgctgaa actattctca tttttgtatc tggtttactc cttgcaacaa
     8641 tagatattcc tttgatttct tctctcaatt ctttatctgt cagtacatct atataggttg
     8701 cttcaactgc ctttttacca tcatttaata atcctaattc atttccaata gctgttgctg
     8761 tattgatatt atctcctgta agcattttta catctactcc tgcattaaat gctttatcaa
     8821 tggactcttt gacaccttct cttaatgggt ctattattcc tacaaatcca ctaaaaacta
     8881 aattatcttc cttcatgtaa cttcttgagc ttcctattaa gttcaattca ccttctgaag
     8941 ttattgcaac ttctgtttta gaattactca tttttttata agcaaatcct aatgttctca
     9001 tagatttaat ttgtaatttc ttaatctcat ctaaaatact tttctctatt tttgaagtta
     9061 ttggaacaat attttttcct tgttgcacat aggaacattt ttttaataat acttctggtg
     9121 ctccttttga aagcaatata tctgaatcct cttgacgaat taatgttgac atcttctttt
     9181 cttctgaact aaatggtatt tgtgatatca aatacgtttg ttttctcatt tcattatagt
     9241 tcttatcatt atgatataaa agtaatgcac actctgttgc acttcctata tatttaaatg
     9301 aattatcttc tttttctata tctgcggttg aatttactat acagttttgt tcaaaatagc
     9361 tgttactttg atactctcct ccacttatat atttaccatc aacatatgct acctctacca
     9421 tcattttgtt ttgtgttaaa gtccctgttt tgtcagaaca tattacagaa acagaaccta
     9481 tagtttcaca ggcttctttc tttgttacta gtgcatttat ctttgccatc ttttgcattg
     9541 ttatagctaa agtaatgttt atcatagttg gcaaaccttc tggaacagtt gcaactatta
     9601 aggcaataca aaccatatat gcatttttag caggttctaa tgattgtaag aaagataata
     9661 cactgcttgt atctaaaatt aacatacctt ttctctgcat ctgtataatc ataaacatac
     9721 acaataaagt agctattaca cctgatattc ctgctatttt agctcccaat ttccctaatt
     9781 taatctgaag tggagtttgc tggtcttcat ttgataaatt ctgtgctatc ttacccatct
     9841 ctgtcttatc tccagtatga gtaacgacca ttatgcctct accatatgct acaagagtcc
     9901 ccccaaatac catatttact tgctttgcag gtattgcatc ctgttcgatt atttctgttt
     9961 tagaataaac aacttccata tctaatacag catcagcatt cttagctaca tcatcagatt
    10021 ctcctgttaa catatcttct ctaagtttca aatttataga ttgtataagt cttccatctg
    10081 ctggtatcat atctccagtt tctatatata caatatcacc tggtactaaa tcattttttg
    10141 atattttaat tatcttccca tttctaagta cttttacttc tatattttct gttaattttg
    10201 acaaggcttg agctgctttt ttagattttc cttcagttat tatacctata gaaattccaa
    10261 taaaaatagc ccctaaaata cctaatgcat catgtagttc tcctacaaaa gaacttatta
    10321 ctgctgctcc tatgagaatt aatatcatag gctctgtcaa actttctccc aactcgtccc
    10381 aaaatgttct accttctttt atagtaaatt catttaatcc atatctaagc ttcctttctt
    10441 ctacctcatt atcatcaagt ccaatttctg gatttgtttt taagtatttt aagacttctt
    10501 tggtaggttt attataatac ctcataactt tatccccctt gttaattatc tataaggaat
    10561 tatatgctta tgatagcttg tatattactt ttcaaaaaaa taaggtgttg attaatcaac
    10621 accttatagt ggttatatat tattaaattt tatgttatag gctttataaa ttgtgagtta
    10681 tataaatcaa aatatttaat cttcaaagta cccttctgga tataatggga acttagatat
    10741 aagttcttgt gcttctgctt tacattcatc taatacttcc ttattatcaa tattttctgc
    10801 taccttattc attattcctg ctatctgtat aacttctttt tctttaagac ctctttgaga
    10861 tattgatgta agacctatac gaacaccact agttacgaat ggtgattgtg ggtcatcagg
    10921 tataacattt ttattaactg ttattcctac atattcaaga gctttttcaa attgttttcc
    10981 tgtaagattc ttgcttctta agtcaaccat tactatatga ttatcagttc ctccacttac
    11041 aatactaaaa ccatgtgaaa tcagttcaga ggctagagtc tgagcattta ctacaacttg
    11101 ttccattatc tttttaaatt tatctgtacc taaatacaat agtgaaaagg cttttgctgc
    11161 cactgtatta agatgtatag aacctaatgt tcctggaaaa acacctttat caagtttctt
    11221 tgcatgttct gctttacaaa gtataatacc acttcttggt ccacagaaag tctttgttgt
    11281 agatgaagaa acaaaatctg cataaggtac agggctaggt attactttag ctgcgactaa
    11341 tcctgcaaca tgtgccatat ctaccataaa gtaagctcct acttcttttg caacctttga
    11401 tattctttca taatctatta atcttgggta tgaacttgcc cctgcaatta taagttttgg
    11461 tttacattct tttgcctttg cttcaagagc atcataatct atacgtccag tatttggatc
    11521 caccccataa aattcatatt tataaatatc atgtaaaaaa ttaactgtgc taccatgtgt
    11581 taagtgtcca ccttggtcaa gacgcatact taagacagtg tcatttggtt ttaatattga
    11641 tgaatataca caatagttag ctgtagagcc tgaatacggt tgtacgttta catgttctgc
    11701 tccaaatact tcttttgcac gttttattgc tagagtttca agtttatctg cttcttcaga
    11761 acctgcttga aaacgagccc ctggatagcc ttcttctgtt ttatttgtaa atacacatcc
    11821 acttaattca agaacttctg ttggtgctgt actttcagaa gctatcatct caatattatg
    11881 ttcttgtcta accaattcgt tagctacaat cttgtataac tctgggtctg aaatttttgt
    11941 tgtttttaac ataataatcc tcctcttaat taaagaatat attttattag attaatcttt
    12001 attaataaat ttattataat ggtctataaa acgtaagata ttcctccacc aggtcctaat
    12061 ggtatgccaa tactcatgaa tagtaacaat attattacct ctattaatgt aaatactact
    12121 gagtatggaa tcatattcga tatgacagtg cctataccta tatctttatc atatcttctg
    12181 gcaaaagcta atataactgg aaaatacgga aaaagtggac ttattggatt tgttgaagca
    12241 tctccaatac gataagctat ttgagttaat gctggattaa aatctagtag cataaacatt
    12301 ggaacaaata taggtgctaa aattgcccat ttcgcagaag cacttcctac aaataaattg
    12361 ataaatccag ataacaatat aaatcctatt atcaatccat aactaggtat ccccgactct
    12421 gccaatactt ttgctcctgt tattgataaa attgttccca agttactaag attaaataaa
    12481 ttaataaatt gagatgctat aaatgctaga actatatatc ctcccatatc actcatacta
    12541 ctacccatca tagatactaa ttctttatca ttttttatct tattacttac tcttccatat
    12601 actaaccctg gagttaaaaa tatgatagta attattggaa ctattccctt cattaaaggt
    12661 gcattgcttg ataatataga accagtttct gggtctttca taaatgcatt ttctcctata
    12721 cataaggcaa ctattattac cacaatagct aatagtgata atagagcgta ctttatacct
    12781 ttattttcaa gctcacttaa ttcctccata tccttatcat ttactgcatc actatatttt
    12841 gaaaaacgag gagctataaa tttttctgtt acaaatgctc ctgcaaaagt caaaattatt
    12901 gctgatacaa aagcaaaata aaggttcatt gctggatttc cttgataaga tgaatccatc
    12961 atttgagctg caggtattgt aaatccagct aataatacat ctgtagtact aagcataaca
    13021 tttgctgcaa atcctcctgc aacccctgca aaagctgcaa acatacctac aagaggatgt
    13081 cttccaattc ctaaaaacac aagtgctgca agtggtggta atattataaa tcctacatct
    13141 gctgctgtat gtgataacat acctgcaaat acaattgcta cagttacaag tttttgtgga
    13201 actttactta tccctttttt cattaacact tccataaatc cagatttctc agcaacacct
    13261 gctccaagca ttgtaaccag cactaatcct aatggagcaa atgattggaa gtttgtaact
    13321 atactaccta agtatgattg aatctgttct ttctctaata gatttactac ctttacaact
    13381 tcttttgtag aaggatgtat ctcttctact cctaaattag atataattgc tgacaatatt
    13441 acaacaatta tgcatagtaa taaaaatatg gttactgggt ctggaagttt atttcctgct
    13501 ctctctataa catttagtgc cctcattaat aatccactat ctttgttttt ttcctgtgaa
    13561 ttaattttcc tcaaactaat cccccctcat aaactttatc tccagaaatc ttctatctta
    13621 aattttattt ttttaattta tccatataat ttataacaga ctgacatagt acatttgtac
    13681 ctaccaatat ggaattttta tcagctttaa atttaggatt gtgccactga tacatacaat
    13741 cttgctcttt gttgcctact cctaaccagt aaaaaaatcc tggagctttt tccataaata
    13801 tagaaaaatc ttctcctcct gtagaaggta ctggatcaac tatagcatct tctgaataca
    13861 aatcacacac agacttacag gctattgtgt acatctcttt actatttata acggctggta
    13921 aatcaaataa ataatctaac tttgcagttg cttgatatcc acgagctatc tcacaaacta
    13981 ttttttctaa attttcagat attttctttc gtacttcatt actaaagctt ctacatgtac
    14041 ctgtcatatc tactttttct gaaattacat tatttgcagt tcctccattt atagtaccta
    14101 ttgatataac agctgattct aatggactta tgtttctact tactatggtt tgaatgccca
    14161 ttataattgc acttgatgct actatcgggt ctattgttct gtttggtata cctccatgac
    14221 ctccaaatcc atttacttct atcttaatag tatctactgc tgccattaac ccacctaact
    14281 taacacctat ttttccacaa ggtatatctg gatgattatg aagaccaaat atagcatcaa
    14341 cttttggatt ttccattact cctcgttcta ctaacatctt tgcaccttta ttgacttcct
    14401 cggctggttg aaatataaat tttacatttc catgaagact atccctaata tgagacaata
    14461 catatgcaca tcctacaagg caagatgtgt gtatatcatg tccacatgca tgcattatac
    14521 catcatatct ggatttatac tcaatatcaa cctcttcgca tattggcaat gcatcaatat
    14581 ctcctcttag tgctactgtg ggaccatcat acttcccttt taataaccca acaactccgg
    14641 tttccattcc taagtctaat atttctatgt ctaaacttac taataaatct tttatcactt
    14701 ttgtagttct aaattcttta aaacttaatt cggggtgcat gtgaatatct gtaaaaaaat
    14761 ctaaaatatc ttctttatac ttttctgaaa ttgaaataag ttcatgcata ctaacacctc
    14821 tttaaaattt tattttttga ttttttgata atttaactat aaattatata agtactaata
    14881 cttataaagt ttataaattt aattttattt atatttatat tttactattt tattttaatt
    14941 aaatatatta tcatatttta caattttttt aactattttg actttttaaa ttcgattata
    15001 taaaaagttt ctttaactaa aaattactaa taagtggctc tactcctgac cttgtgttga
    15061 tttttctcta caggtaattg ctttatacac catacagact aaaattaaaa gtcccatata
    15121 acctgtatat ggatataaaa tgtttactag tttaccaaac ggtaaaaaac cacctataca
    15181 agccaaaata gctattactg ttgttactaa tttaaattta gggtgttcat cttcaacaat
    15241 tctattggtt acagaccata aaagtggaac agctgttgta tagatgccta atattagtgc
    15301 aactgaaaaa catacaccta caataggagc tatattatct gctaaataaa gtgatggtat
    15361 ttctttcatg tacaaatttc ctatatctga aatcatagcc aaatacataa ctataccagc
    15421 agtcataaac acgattccac ccaataaacc tccccaaaaa gcatcctttt tactttcaat
    15481 ggaagctccc attcctgtta aaaaaacaat cattacaata atatttaatg tattatatat
    15541 tacaccggaa atgattggac tttctgatgc ttttgtaact tcaatgtttt tcatagcttc
    15601 tccaaccata gataaaccat caaaatgttt aaacaagctt attaatccaa cagcaattgt
    15661 aaatacggct atcactggtc caatacgacc aagtatactt actaattttt ctaaacccat
    15721 agacactgta attaaagtag caacagccat caacactctt cctaagtaag gatttatacc
    15781 ataatattca gttaaagttg aacctgcacc tgaaatcatt actataagaa tcccaaataa
    15841 aaatattgga ctaaaccatt cgtaaaattt tcctaaaatc ttaccacaat aaaaagtgta
    15901 tattcttgtt ggttctttaa gttttaattc tctacctcta gttataactt cagcaccaag
    15961 aaatgaaaac aacaccattg atattaatgc tccaactaga cccatatacc cttgtgaggc
    16021 aaaaaattgt agaatttctt gaccagtagc aaacccagaa ccaattgctg tcgctaaata
    16081 agcacctgca atacagataa tattctttat attcactttg tctgacatat taaattacct
    16141 ccattataaa gttaccttcc tctaagaaat ataaactcac agtaaattaa atgaaaattc
    16201 tgtcttttta gaatagtatt tgatttactt ttttattttg tatatctttt tctttttatt
    16261 tatatatatt tttttatata aataggtaca gcttttataa ggtttaatat tattaaatat
    16321 gtttaaagcg ctttaatttt tattataatt aaagtatact tattcagaca tactcaaaca
    16381 attatctata ttgatttttt atttatcaat ttcaattttt agtattcagt ttataaactt
    16441 ttggagtcat tccttttttt atcttaaagt ttttataaaa gttatttagg ctattatatc
    16501 caactatttc tgcaatctca tttatacttt ttgtagtatt ttcaagtaac tcaatagatt
    16561 tttctattct tatatgattt agatatgata taaaagttgt attcaaatta cttttaaaaa
    16621 tcctagatat attagatgga ttggttaaaa ataatctagc acaatcattt aactttaagt
    16681 cattactata atttagattt atatattcca gtatctcatt taataaatca ttattggtat
    16741 attgatatcc aataattttt tctacagatt tagtaaattc tttcatgtcc aatggtttaa
    16801 ggagtatatc ttttacattt aatctcaaag ctttttgagc atattcaaaa tagtcaaaag
    16861 cagttattac tacaaagttt atttttttag ataagtgaga tgttttttct attacttcta
    16921 acccattata tattggcatt tgaataccta taaaaactat gtctggctct aaaccagtga
    16981 ttttatctac tgcttcatca ccagaggttg ctttaccaac aatttttatt ggcatatctt
    17041 tcatttctat aaaatgtgtt attattttta gaacagcttc ttcatcatct attattagtg
    17101 ctcttctcat gagaatacct cccttttagc ttaagaatat aaaaaagtaa gatatataaa
    17161 acaacataaa aacatctaat atatttctta acatttataa aacttattaa tttttcacaa
    17221 gttctataca tactccttat aattttcact aggaatttta atcattatgt aagttccctc
    17281 attatcactc gactttatat ccatatcaaa attatttcca taagcagatg tgaattttgt
    17341 atatattaaa gacaaaccat gaccattatt aatacgaata cctgccttta ttttgtctat
    17401 attattttta tctaaaatag ttccattatt atgaatctct ataatagcat actgagaatc
    17461 taatcttgca attatttcaa ttctcttttc tgtgtcaata tctctagatc catgtgtaaa
    17521 tgcattctct acaataggtt gaataaagtt aaaaggcaca gaaaggttat ataacttctt
    17581 tggaataaga tattttactt taagtgcatc tccataacgt aatttctgta aattcaaata
    17641 attttcaatg tataatattt cagattctaa ttctacaaat cttaaatcag acctcattgt
    17701 atatctaaac attcttgata aatctattat tgcactatac aaatcataac taccatcatc
    17761 tagtgctatg cttaccattg aatttaaggt attaaataaa aagtgatgat ttattcttaa
    17821 attagtaacc atatcttgag ctacctttaa attcatttca agttgctctc cataatgata
    17881 tgttcttgct atagtcttct ctttttcttg taaatccttt cttatatcat taaatgaaca
    17941 aaaattaatt atatttttag caatttgatt gagtaacctt ttcatagttc tagctgcacc
    18001 ttctggtata tcatataaat tcctatgtcc aacccctaaa cttgaatcag acattagaat
    18061 atatccacct cttataaccc ctatgctatt atttttatat attatagaaa tattatatat
    18121 ggttatacca tacttacatg taaattgatt agcacttttt tgattcatgc aatcacaatt
    18181 ttgtgggaat ttcattggat tacatttttc aaaacaaaat aaagggaaat atggattttt
    18241 taaaactata ttccagtaat catcataaat aatcaaaggt attttaaatg ggtctataaa
    18301 ctgacctcgt attgattcaa ctgctgcgta tagatttaag gtattttcac tataatttac
    18361 tggagtattt tctattttat tatttttatt aaattcaaga atctcacttg tttcaaaatt
    18421 gtttacaata tttgatacta ctaactcttt tatttctcta tctcctgtat ttatagtttc
    18481 atgtgtgcta ccagattcta tataaaaaat catcccttga taaaatggct tccagacccc
    18541 atttataata tacaaaccct ctccctctaa aatatatatc atctgttctt gaccataatg
    18601 tacatgatta aaatgtttct taccaggcat agttactgca atgtatatat tcatagcttg
    18661 ttttggatta ttttcatcat aagtatgttt ccattcaata tatccccact cagtattttg
    18721 aattttcata aaatattcct ttcctgtaaa ttatttatgt tcatattata aaaaattagt
    18781 aaacaaaaat gtgtacatac tctttcatat gatagcttac tatatcaata ttcattagtt
    18841 tgtacacatt tttatattaa ttattatttt ttaattactt ctatattatt gtattacttt
    18901 tatattaatt attttttata gattatagtc tcacttctat gttaattatt ttttattact
    18961 tctatattag aagtgttgct taaaaataca taatttgaat cttatttaat aacagaaact
    19021 cccatatatg gtcttaaaac ttcaggaaca atcacagagc catcttcttg ttgataattt
    19081 tctaatatag ctgcaacact tcttccaata gctagcccag aaccatttaa tgtgtgagca
    19141 tattcagctt tagaatcttt atctctttta aatcttattc ctgcccttct agcttggaaa
    19201 tcttcgcagt ttgaacaaga tgaaatctca acatacctat tgtaacttgg catccaaact
    19261 tctacatcat atttaaaaga agccgtaaat cctaaatcac ctgtacatat ctttactact
    19321 ctataaggca gattcaataa ttgtagcata gtctccgcat tattagtaag cttttccaac
    19381 tcattgtaag attcttctgg tgcaactatt ttaaccattt ctactttatt aaactgatgt
    19441 tgtcttacca aacctcttgt atctcttcca gcagaaccag cttctgacct aaaacaagga
    19501 gtataagctg tataatttat tggtaattgt tcaaatttaa gtatttcatt agcatgtata
    19561 ttagtcaaag gaacttctga tgttggaatc ataaaatagt ccagtccttc tattttaaac
    19621 atatcttctt caaacttagg taactgtcct gtacctaaaa aactatttct atttgccata
    19681 aatggtggaa taacttctgt gtatccattt acctttgtat tcgtgtccaa gtaaaaattt
    19741 aataatgctc tctctaatct agcacccaag cctttgtaaa gagtgaatct tgaaccggtt
    19801 attttactag ctgtttcaaa atcaagtata cctaattctg ttccaatttc ccaatgagcc
    19861 ttagattcaa aatcaaattt ggtagtctct ccccaacttc ttacctcaac attatcatca
    19921 tcagtttcac cttgtggtac tcctgggtgt ggtacgtttg gtattctcat taatagatat
    19981 tccatcttgt cttctacttc tttaagattt tggtctatta ccttaatttt atcagataaa
    20041 tcttttagtt taatcttttc attttctaca tctttacctt cttttataag atttggtata
    20101 tttttagatg ctgtatttaa tttatttttc ataacttcaa cttcttgaag aatctttctt
    20161 ctttcatcat ctaacttaac tacttcatca aggttaaaag ctttttctcc ccttctttcc
    20221 attgcttttt ttatacaatc taaattttct cttattcttt ttatatcaag cattttttcc
    20281 tcctcagtac ctaagattgg attttttctc ttaatctcaa ataacttaga taatatgttt
    20341 tatgcttgag gttaagaaac ctttttttat ccgaacctat tatttttatt tatattttac
    20401 tcactttttt atctattgtc aaattatatt taattaaata aatacaattt aatttcaaga
    20461 gaatatttct agatttaact tcattataat tatatcgatt tttctaatca attatctatt
    20521 ttgacttttt ctttatcaat cttgtatagt tattaacttt catataagtt tttttatatt
    20581 ataatataga cctatatatc tcttatttgg atagtattat tttaataaat gatttaagta
    20641 taggtatgaa attaaatttt ttagaactga ttataagaaa tactcaaaga aaagtagtaa
    20701 tttccccaga ttcacaatat gttttatatg aaatgttgta aggtaatcta tttaatgaat
    20761 ttaaatttgc tattataaac ttagctccaa aatctactgc aaagtaatat acatattcgt
    20821 tttatactaa taactgcaac taataatta
//
LOCUS       DAFNNB010000059        20824 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0059, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000059 DAFNNB010000000
VERSION     DAFNNB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0059"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(100..942)
                     /locus_tag="L3M60_001849"
     CDS             complement(100..942)
                     /locus_tag="L3M60_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6226518.1"
                     /translation="MIIQQIYNNNVVLVLDENIKKELILTGCGIGFQKKKGQEIDKNK
                     IERTFIIQDESFLDKIGKLASQVDEKFFAISTEIIAYAEENLNTKLYEYIYVALTDHI
                     AFAIKRYHENIIIKNELLHEIKRIHKKEYEIGKWAVDYINKEFGVKFPLDEAGFIAMH
                     IVNSNYKGSSKESLLITKIVKDILNIIRYYYRVEFKEDDINYDRLLTHLKFFAKRLVK
                     REKINDTSNEIIDIIKVKYEKDYNCAYKIKTHVEKNYDYYVSQDELLYLTLHIKRVIS
                     VLNL"
     gene            complement(1202..3244)
                     /locus_tag="L3M60_001850"
     CDS             complement(1202..3244)
                     /locus_tag="L3M60_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HBN6226519.1"
                     /translation="MSILVTLLGLLVCTTISTVFSKKWNNIPLAIYQIVLGIILSILP
                     FKFLFSFNPEIFVICIIAPLLFSEAQNVSRKELLELRKPILLLAFGLVLTTVFAGGIF
                     IHFLIPGMPLSVSFALAAVISPTDLVAVKSITQGLNFPKNMMSILEGESLLNDAAGVV
                     AFKVAVLATVTGVFSIEEAGIQFIITAFGGIIVGSILGYIIIKIRLSLHKWNLEEIPM
                     VIVIQIMTPLFIYFVAEEVGVSGILAVVMAGIAHGIEKEHLQNTTTKLRIISDNTWYV
                     LEYVLNGFVFTLLGFLLPSIYTGLSSKNEDMTLKLTFISALIVFILFVIRFIWVYLWH
                     DSFIKTKKNPLNNFFVGFLGFKDEEAVKENISRCKYSLIVATCGVHGTFTLATALSIP
                     FHLANKDVFPMRDTVLFISSEVILISLILATVLLPRLLKSNSQESEHLLSDKEAYKLI
                     LKEAILKLSNEKAQEAQLVIHDLNEQLVDSEKGILNTPDNKLISMMIAEADKQGLLAV
                     LKLLEDGKISDRAFKLYRFYITKSRKSVQKSIFKIIKLKISMWTIDRKVKKEVKEKIT
                     KIVSENKEIIKEFHYAYALSTKTAISFINKNTDENNRHEALISIRFYNRYLNWITRKI
                     QNDDKFESRVEYYRVMAIQSQRDSVQQLVENKQISREVAKELLENILYDEMMMIES"
     gene            complement(3395..4366)
                     /locus_tag="L3M60_001851"
     CDS             complement(3395..4366)
                     /locus_tag="L3M60_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBN6226520.1"
                     /translation="MKKEESLLKVEGLKKYFEVKSGLFGKEIKFVKAVDGISFDLKKG
                     ETLAIVGESGCGKSSAGRTILRLIEKTEGKVEFNGIDIYKLEKEELRKLRPKMQMIFQ
                     DPYSSLNPRLNVEQIISEAVIEHDLVPKNEIKDNILNIVESCGLSKYHLNRKPGEFSG
                     GQRQRIGIARALALNPEFIVCDEPVSALDVSIQSQIINLLMDLQDKYKLSYLFISHDL
                     SVVNHIAHRVCVMYLGSIIEIATKEELYLHSQHPYTKALMSAVPVQDPTIKKNRIILK
                     GDIPSPTNPPSGCKFHTRCPYCKEICKKEVPVLKEISRNHFVACHLI"
     gene            complement(4367..5344)
                     /locus_tag="L3M60_001852"
     CDS             complement(4367..5344)
                     /locus_tag="L3M60_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226521.1"
                     /translation="MSKALIEVKDLKVYFHTDKGIVKSVNGVSFNINEGETIGIVGES
                     GCGKSVTAMSLMKLLPTSKIEGGEIIFQGKDILKMNENELMRIRGNEISMIFQEPMTS
                     LNPAFTVGSQIIEGIMLHQDLSKEEAKKKVIDMIKLVEIPRAEEIYNSYPHELSGGMR
                     QRIMIAMALSCNPKLLIADEPTTALDVTIQAQILDIMKNIKEKLNTSIMMITHDLGVV
                     AEMCDKVVVMYAGKIIEVAEVVELFKNPKHPYTIGLLKSKPVLGKNKDKRLYSIPGQV
                     PNPIGIPDSCYFSDRCEKVCDKCRTQIPPLIELNSGHSIACWLYKKEEI"
     gene            5563..7122
                     /locus_tag="L3M60_001853"
     CDS             5563..7122
                     /locus_tag="L3M60_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226522.1"
                     /translation="MKFKKLASLILVSALMLTFTACASNDKEKSNGGKSGGTVVIPMA
                     SDPDIINGAFANVKEDSLASNIVYAPLYTYEKGNLVNYLAEKVDFKDSKELTIKLKSN
                     LKWHDGKPITAEDVLFTFNTVLDEKQNSPSRQYLLVGEKPVKVEKIDDLTVKITLPTA
                     SESFLYGISKISPIPKHVFEGESNIAKSEKNNNPVGSGAFKFKEWKKGESIVFEKNED
                     YFGGEPKADSIALKIIPNEASQEAALNNGEISLMKTSAEGYEKAKSNSNLQTYTYSEE
                     RLNYIVFNQNISNMANKEVRQALSYALNRNEMIESAYGKEGSVPAKSILVPEADFYTE
                     EGVEGYDQNTNKAKDLLDKSGVKIDKLKIGYNTGRFGHKNYALVAQQELKKIGIEAEI
                     VPYESKAFFNILFSNSTECDMYVNGYAWGLEPNPYRGMFETGQYCNQTKYSNAKIDAL
                     WEKGFTELNKEKREEIYKQIQQDISKDAPIYTIDYEQNLMAAQKNLKGIEDAKPSPAI
                     LFEDWSKLYVE"
     gene            7228..8187
                     /locus_tag="L3M60_001854"
     CDS             7228..8187
                     /locus_tag="L3M60_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226523.1"
                     /translation="MLKLILNRVKVLIPMLILISILSFGLLELAPGDPADAYINPLMS
                     AQDIENIRVNMGLDKPVYVRYLSWLKNTLSGNLGISYINHMPVTEQIMEKMGNTFILM
                     GTSLIFSILVAIPLGIFLAVNKNSITSKISSIFNYIGVSIPSFWIGMILISIFSVKLN
                     IFPSGGMHTIGNDSIGDLVKHLVLPVITLGLYNTAIFTNYVEASVNEQLKKQYVVTAR
                     AKGLSEKVILFKHVLKNSLTSLVTILGMSIQKLVTGAFVTEVVFSWPGMGRLMIDSIF
                     SRDYTVIMAITMLSALFLILGNLVADILYLLIDPKIKSSKGGF"
     gene            8190..9002
                     /locus_tag="L3M60_001855"
     CDS             8190..9002
                     /locus_tag="L3M60_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226524.1"
                     /translation="MFTNILKELKKDKSFAFACITLSIIIILIIASFILNIDSNAIDT
                     TSKLQEPSLKHIFGTDELGRDYFSRALYGGRISLSVGILSMLISVVFGTAVGVISGYI
                     GGKLDQFLMRLVDMLMSIPSFLVLIVLNTYLTPKISTIILIIGFLSWMEVSRIVRGET
                     LKIKENEYCLAAKATGIKTRNILLRHIVLNLKETIVVAGSLNIANAILTESALSFLGL
                     GIQLPLASWGNMLQDAQKYIFDKLYLAVFPGLLIFLTVMSFTIISKKFSSNT"
     gene            complement(9217..10119)
                     /locus_tag="L3M60_001856"
     CDS             complement(9217..10119)
                     /locus_tag="L3M60_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6226525.1"
                     /translation="MDIKQLKYFVEIVKSGFNLSIASKTLHISQPALSQIIKNFEESE
                     DVYLFERYKGRLNGLTPAGERFYVNAEHIINEYKNMMEDLREDSVQFKGKIRIGIPPL
                     ILGIVFSDVVAQLVANNPDIEFDIVERGAYDLSRMLILQELDYAVLLHPHKIDKSVVT
                     EHVLQEDELTAFLNINHPLAKKDKIDWNDLNEQLLAIFNPTFMIHHKLMQKFTDENVK
                     LKRYIMSGSWDFLLLSTTNSDFITILPSPVHDFFENSKIIEKPFNYPITWKVVLCRPK
                     KERYSHVDQHVFKFIIDFFSKKNS"
     gene            10472..11650
                     /locus_tag="L3M60_001857"
     CDS             10472..11650
                     /locus_tag="L3M60_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000786547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBN6226526.1"
                     /translation="MKDVVIVSAVRTPIGNFGGVFKNTSAVQLGTITVKESISRIGLN
                     LSEINEVIIGNVLQAGLGQNVARQIAINAGIPNSVPSYTINKLCGSGLKSVQLAAQSI
                     ASGENDVVIAGGTENMSQAPYIVPTARFGNKMGDITMVDSMLTDGLIDAFNQYHMGIT
                     AENIATKFEFTREMQDKLALESQNKAEKAIKNKRFKEEIIPVNILIRRGKMETIDTDE
                     YPKLGMTFEGLSKLKPAFKKDGTVTAGNASGINDGAAMLILMSKQKADELGIKPLVKI
                     KSYASAGVEPEIMGTGPIPATRKALKKAGLSINDIDLIEANEAFAAQALAVKNELQID
                     SSKLNVNGGAIALGHPIGASGARILVTLIYEMQKRKVKTGLATLCIGGGQGISMVVSR
                     "
     gene            11659..12315
                     /locus_tag="L3M60_001858"
     CDS             11659..12315
                     /locus_tag="L3M60_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HBN6226527.1"
                     /translation="MNKIVSIDEALSHVKDGDTIMVGGFMANGSPENLIDYLCSKNIK
                     DLTLICNDTGFIDKGVGKMVVNKQFKKIIASHVGLNKETGRQMNNNETDVELVPQGTL
                     AEQIRAAGYGLGGILTPTGIGTLVEEGKQKIVVDSKEYLLEKPIYADVALLFASKVDK
                     AGNLVYKGSMNNFNNLMASAAKTTIVEADEIVEIGDIDPNEVNTPGIFVNYIVEGGNL
                     "
     gene            12315..12980
                     /locus_tag="L3M60_001859"
     CDS             12315..12980
                     /locus_tag="L3M60_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HBN6226528.1"
                     /translation="MDKLEMQEYIANRVSKELKDGAVVNLGIGLPTKVANYIPDDVNV
                     ILQSENGFLGLGAAEDGNSNDETIVNAGGQPVTILPGGCFFDSATSFGIIRGGHVDIT
                     VLGALQVDKYGNIANYMIPGKMVPGMGGAMDLVTGAKKVIVAMEHTSKGSAKILNNCT
                     LPLTATNAVDLIVTEMGVMEVTSDGILLKEINPAFTLDDVISSTEAPLILSDSLNGNI
                     SVV"
     gene            13085..13861
                     /locus_tag="L3M60_001860"
     CDS             13085..13861
                     /locus_tag="L3M60_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBN6226529.1"
                     /translation="MLKDKVVFVTGAASGIGKQIAESFLKNGAKVMFSDINQEALNKT
                     TTELQQEGYDCMNVKCDVTKEEEINQAIDKTVEKYGRLDILINNAGLQHVSMIEDFPT
                     DKFEFMIKIMLTAPFIATKKAFPIMKKQGFGRIINMASINGVIGFAGKAAYNSAKHGL
                     IGLTKVAALEAADSGITVNALCPGYVDTPLVRGQFNDLSKTRNIPLENVLEEVLYPLV
                     PQKRLLDVQEIADYAMFLASDNAKGITGQACLLDGGYTAQ"
     gene            13923..15257
                     /locus_tag="L3M60_001861"
     CDS             13923..15257
                     /locus_tag="L3M60_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBN6226530.1"
                     /translation="MSIIAPIGIIIGIIAIIYFSVKEIHITIAAPIATLIVVLLNKMD
                     VVTSMLGADKNNYMGALGNYIMSYFAIFLLGSILAKFMEESGATVSIADFILNKFGSN
                     HPYRVLVAIFIVSFILTYGGISLFVVMFAVIPLARTLFKKLDISWNLIQIPLWLGIAT
                     ITMTIIPGTPAIQNVIPIQYLNTSLTAATIPSVAGSIGCATFGLVYMKYALNKSIRKG
                     ETYATYTSELEEESLARELPGFFASIFPLLALVIIAITGSTFGSEFMKKNVIYIALIV
                     AILLSAFLFHKFLSSKIQTLSIGASGSIGPIFATSSAVAFGAVIMAAPGFDVFSKLIM
                     SIPGSPLISLTVLTSTMSAITGSSSGALGIVMPNFADYYLSTGLNPELIHRVATIASN
                     ILTIVPQSGVFLTFLSLSKLTHKTGFKETFIVVAVGSLIAEIIVITLGLLMY"
     gene            15758..16711
                     /locus_tag="L3M60_001862"
     CDS             15758..16711
                     /locus_tag="L3M60_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein"
                     /protein_id="HBN6226531.1"
                     /translation="MRKNIPTKLKTILALGVLTLTTASSAVNASALSLENNVNTSNKN
                     LVYSNTRYKFSAKPNCSIKPELKPDKDNSCKDKNHSDKNCNNTNKPDNNKPESNKPDD
                     NNNSGSTDKPDNNKPESNKPDDNNNSGSTDKPDNNKPESNKPDDNNNSDSTDKPDNNK
                     PESNKPNDNNNSDSTDKPNDNNNSGSTSENFSAYQKEVVDLVNIERSKAGLNPLTLDS
                     SISNVATKKSQDMIDNNYFSHNSPTYGSPFDMLKKFGISYKTAGENIAMGQKTPKEVV
                     NAWMNSEGHRKNIMNPNFSKIGVGVAQKSGGSIYWTQIFVG"
     gene            complement(17115..20654)
                     /gene="nifJ"
                     /locus_tag="L3M60_001863"
     CDS             complement(17115..20654)
                     /gene="nifJ"
                     /locus_tag="L3M60_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBN6226532.1"
                     /translation="MAKFMKTLDGNTAAAHVAYAFTDVAAIYPITPSSTMAEVVDEWA
                     SQGRKNIFGQTVNVVEMQSEAGASGTFHGSLQGGALTSTFTASQGLLLMIPNMYKVAG
                     ELLPGVFHVSARALASQALSIFGDHQDVMAARQTGCVLLASGSVQEVADIAPVAHLAA
                     IEGRLPFIHFFDGFRTSHEIQKVELLENEDYASLLNFEAVQAFRDNALSPNHPVARGT
                     AQNPDIYFQTREASNKYYQNIVGIVEKYMEKMSNLTGRKHSLFDYYGAEDAKYVLVAM
                     GSVTETIEETIDYLNAKGEKYGLVKVHLYRPFSMKHFLDAMPSTVERICVLDRTKEPG
                     STGEPLYLDVRDVFYGKENAPMIIGGRYGLGSKDTTPSDIKTVFDNLVSEQPKNGFTV
                     SIVDDVTNTSLTPSEPIKIASKGTIRCKFWGLGSDGTVGANKQAIKIIGDHTEKYAQA
                     YFDYDSKKSGGVTMSHLRFGDSPIRATYLIDEADYIACHKQSYVYQYDLLKGLKKGGT
                     FVLNTIWDQAGLEEHLPAHMKQYIAKNDIKFYTVNAVKLGQEIGLGNRINMIMQSAFF
                     KLAEIIPEEDAVKYLKDSIVKAYGKKGEKIVNMNYAAVDAGINALVKVEVPASWENAV
                     DADKGEVKEPEFIKNILRPMTAQEGNNLPVSTFNGIEDGTFPCGTAAYEKRGIAVDVP
                     EWIIDNCIQCNQCSFICPHACIRPVLVTEEELANAPEGFEAKKALGKGLEGLKYRIQV
                     SPLDCTGCGNCADICPAKEKALVMKPIDTQLDTELDNWAFAVNPDEVAPKVDVMPANT
                     VKGSQFRQPLMEFSGACAGCGETAYIKVVTQLYGDRMMIANATGCSSIWGASAPSIPY
                     TCNHEGKGPSWANSLFEDNAEYGFGMYTAVKQIRNKIVDAMTELVSMDICEDAKAVFT
                     EWLDSRNDGEASKVASAKVVELLEKPACDCTDEKAKELVKAIKDRKDYLVKRSQWILG
                     GDGWAYDIGYGGLDHVLASGEDVNVLVFDTEVYSNTGGQASKATPAAAMAKFAASGKK
                     SRKKDLGMMAMTYGNVYVAQVAMGADKNQFIKAVLEAEKHDGPSLIIAYAPCINHGLK
                     EGMGRTVANEAQAVACGYWHLYRFNPEVKAEGKNPFTLDSKEPTASFKDFILAQVRYS
                     AIAKQFPEEADGLFAKAEADAKERYEGYKKLAE"
BASE COUNT         7202 a         3265 c         2932 g         7425 t
ORIGIN      
        1 aatgttttaa aattattgtt ttatattaaa ttttaaataa tatattaaaa ttaaacataa
       61 tatactaaac ctatacattt tgtattaaac tactctttat tataaattca atacagatat
      121 aactcttttg atatgtaatg ttaaataaag tagttcatct tgacttacat agtaatcata
      181 attcttttct acatgagttt ttattttgta tgcacaatta taatcctttt catactttac
      241 tttgattatg tctataattt cattacttgt atcattaatt ttttctcttt ttactaaccg
      301 ttttgcaaag aattttaaat gagtcagaag cctatcataa tttatatcat cttctttaaa
      361 ttcaactcta taataatatc ttataatatt tagtatgtct tttacaatct tagtaattaa
      421 caatgattcc tttgaacttc ctttatagtt tgaatttact atatgcattg ctataaaacc
      481 agcttcatcc aatggaaact ttaccccaaa ttctttattt atgtaatcta cagcccactt
      541 cccaatctca tattcttttt tatgtattct ttttatctca tgtagtaact cattctttat
      601 aattatattt tcatgatatc ttttaattgc aaatgcaata tggtcagtaa gtgccacata
      661 aatatattca taaagcttag tattcaaatt ctcttctgca taagcaatta tttcagtact
      721 aatcgcaaaa aatttctcat caacctggct tgctagttta ccaattttat ctaaaaagct
      781 ttcatcttgt attataaaag ttctttcaat tttattttta tctatttctt gacctttctt
      841 tttttgaaac ccaatcccac atcctgttag tattaattct ttttttatat tttcatctaa
      901 aactaatacg acattattat tataaatttg ttgaataatc ataatcaata tctctcccaa
      961 aatacaattt cccttattca taaaaaaata cctaaattaa gatacaattc acctgtatac
     1021 cttaacctag gtattgcctg cttaccagta acaatccagc ctgttaacgc ttatatattg
     1081 tattcaattt atggttttat tctattataa ataaatatat atgtcaagta aattattgaa
     1141 aactatttcc tattttttgt attaattcta acatcaaaaa tcaaatttta ttccattata
     1201 tttatgattc tatcatcatc atttcatcat atagaatatt ttccaataac tctttagcca
     1261 cttctcttga tatttgctta ttttcaacta attgttggac actgtctctt tgactttgta
     1321 ttgccattac tctgtagtat tctactcttg actcaaactt atcatcattt tgaatctttc
     1381 tagtaatcca attcaaataa cgattataga atctgattga tattaaggcc tcatgtctgt
     1441 tattttcatc agtattttta tttataaatg atatagccgt tttagtagaa agtgcatatg
     1501 catagtgaaa ttctttgatt atttctttat tctcagacac tatttttgta attttttctt
     1561 ttacttcttt ctttactttt ctgtctatag tccacatact tatttttaat tttataattt
     1621 taaatataga tttttgaaca gattttcttg attttgtaat ataaaatcta tacaatttaa
     1681 atgctctatc agaaatttta ccatcttcta atagtttcaa aacagccaat aatccctgtt
     1741 tgtctgcttc tgcaatcatc atacttataa gtttattatc tggagtattt aatattcctt
     1801 tttctgagtc tactaactgt tcatttaaat catgtataac taattgtgct tcttgtgctt
     1861 tttcattact aagttttaaa atggcttctt tcaaaattag tttatatgcc tctttatcag
     1921 ataataaatg ttctgactct tgtgaattac tttttaataa tcttggcagt aaaactgttg
     1981 ccaatattaa acttataaga ataacttctg atgatataaa taaaacagta tctctcattg
     2041 gaaatacatc tttattagcc aaatgaaatg gtatagataa ggcagtagcg agtgtaaacg
     2101 taccatgaac accacaagta gcaactatta gtgagtattt acacctagaa atattctctt
     2161 ttacagcttc ttcatcctta aaacctaaaa aacctacaaa aaaattattt aaaggattct
     2221 ttttagtttt tataaaagaa tcatgccata aatataccca tataaatctt ataacaaata
     2281 aaataaatac aattaaagca gaaataaagg tcaatttcaa tgtcatatct tcatttttac
     2341 tactaagtcc tgtatatata cttggtaaca gaaaaccaag cagtgtaaag acaaacccat
     2401 tcaatacata ctccaaaaca taccaagtgt tgtctgatat tatccttaat ttagtagttg
     2461 tattttgtaa atgctctttt tcaattccat gagctatccc tgccatcaca acagctagga
     2521 ttccagatac tcctacttcc tctgctacaa aatatataaa aagtggagtc attatttgta
     2581 taactattac cattggaatt tcttccaaat tccacttatg taggctaagt cttattttta
     2641 taattatgta tcctaaaata cttcctacta ttattcctcc aaaagctgta attataaatt
     2701 gaatccctgc ctcttctatt gaaaaaacgc ctgtaactgt ggcaagtact gcaaccttaa
     2761 atgctactac tccagcagca tcatttaata aagattctcc ttcaagtata ctcatcatat
     2821 ttttaggaaa atttaatcct tgtgtgattg attttactgc tactaaatca gttggagata
     2881 ttactgctgc caaagcaaat gaaacagata atggcatccc aggaataaga aaatgtataa
     2941 aaattccacc tgcaaacaca gttgttaaaa ctaaaccaaa tgcaagaagc aaaattggtt
     3001 ttctaagttc taacagctct tttcttgata cattttgagc ttcactaaat agtaatggag
     3061 ctataataca tatcacaaat atttctggat taaatgaaaa taagaattta aatggcaaaa
     3121 tagataatat aatccctaat acaatttgat atattgcaag aggaatatta ttccactttt
     3181 tgctaaatac agttgaaatc gttgtacaaa ccaataatcc caacaatgtt actaaaattg
     3241 acataactac ctctcttttc aataataata ttatagatta aacttctgta tcaactaact
     3301 taatattaac aacacgaaat tccatcttca tcaaaaaatt ttcaatttat tctattattc
     3361 ttattatata cattaaattt aatttttgaa atatctatat tagatgacat gcaacaaaat
     3421 gatttcttga tatttctttt aaaactggaa cctctttttt gcatatttcc ttacaataag
     3481 ggcatcttgt atgaaactta catccacttg gtggatttgt tggagatggt atgtctcctt
     3541 ttaatataat tctatttttc ttaattgtag ggtcttgtac gggtactgca ctcataagtg
     3601 cttttgtata tggatgttga gaatgtaaat aaagttcttc tttggttgca atttctatta
     3661 tagaacctag gtacataaca caaactctat gtgcaatatg atttactaca ctcaaatcat
     3721 gagatataaa taaatatgag agtttatatt tgtcctgtaa atccataagt aaatttataa
     3781 tttgagattg aatagaaaca tctagtgcgg aaacaggttc atcacaaact ataaattctg
     3841 gatttaatgc taatgctctt gctataccta ttctctgtct ttgacctcca gaaaactcac
     3901 ctggttttct atttaaatgg tacttagaaa gaccacaact ttctacaata tttaatatat
     3961 tatcttttat ttcattcttt ggcactaaat catgttctat aactgcctca gatattattt
     4021 gctccacatt taatcttgga tttaatgaac tatatgggtc ttgaaatatc atttgcattt
     4081 tgggtctcaa ctttctaagt tcttccttct ctaacttata tatatctata ccattaaatt
     4141 caactttacc ttcagttttt tcaattaacc taagaatggt tcttcctgca cttgattttc
     4201 cacatcctga ttctccaact atagccaaag tttctccttt ttttaaatca aaagaaattc
     4261 catctactgc ctttacgaat ttaatttctt tgccaaataa tccagattta acttcaaagt
     4321 actttttaag accttctact tttaataaac tctcttcttt tttcaattat atctcctcct
     4381 ttttatataa ccaacatgca atactatgac cactattcaa ttctattaaa ggaggtatct
     4441 gagttctaca tttatcacat actttttcac atctatcact aaaataacat gaatctggta
     4501 ttccaattgg atttggtact tgtcctggaa ttgaatataa tcttttatct ttgttttttc
     4561 caagaacagg ctttgatttt agaaggccaa tagtataagg atgctttgga tttttaaaca
     4621 attcaacaac ctctgctact tctataattt tgccagcata catcactact actttatcac
     4681 acatttctgc aactactccc aaatcatgtg taatcatcat tatagatgta tttaattttt
     4741 cttttatatt tttcattata tctagaatct gagcttgaat agtaacatct aaagctgttg
     4801 ttggttcatc agctataagt aattttggat tacatgataa agccatagct atcattattc
     4861 gttgtctcat accacctgat aattcatgtg gatatgaatt atatatttct tcagctcttg
     4921 gtatttctac cagttttatc atatctatta cctttttctt agcttcttcc tttgataaat
     4981 cttgatgtaa cataatacct tcaatgattt gacttccaac agtaaatgct ggatttaaag
     5041 atgtcattgg ttcttgaaaa atcattgata tctcattccc tcttattctc ataagctcat
     5101 tttcattcat ctttaaaatg tctttgcctt gaaagattat ttctccacct tctatttttg
     5161 atgttggaag taacttcatc aaagacatgg ctgttacaga tttaccacat cctgactctc
     5221 caactattcc aatagtttca ccttcattta tattaaaact tactccattt acactcttta
     5281 caattccttt atctgtatga aaataaactt ttaaatcttt aacttcaatt agtgctttac
     5341 tcattatttt ctcctctcag caaaattgct taaaataaca tacacctttt gattgtattt
     5401 tataaaaatt taaattacaa cattttatta cattttatta tttttttcta ttaattataa
     5461 tattgtaata gttaatttct atatatattg atttaataat gattattatt atacaatgaa
     5521 aaatatgtgt taaatattat tttatggagg ttttataaga atatgaaatt taaaaaattg
     5581 gcatcattaa tcttagtttc agcattaatg cttacattta cagcttgtgc ttctaatgat
     5641 aaagaaaagt ctaatggagg caaatctggt ggaactgttg taatccctat ggcaagtgac
     5701 cctgatataa tcaatggagc ttttgcaaat gttaaagaag attctcttgc atcaaatata
     5761 gtttatgctc ctctttatac ttatgaaaaa gggaatttag taaattatct agcagaaaaa
     5821 gtagatttta aagatagtaa ggaattaact ataaaattaa aatccaactt aaaatggcat
     5881 gatggaaaac caataactgc tgaagatgtt ttatttacat ttaatacagt tttagacgaa
     5941 aaacaaaatt caccatcaag acaatactta ttggtgggtg agaaaccagt taaagtagaa
     6001 aaaattgatg atttaacagt taaaattact ttgccaactg caagtgaatc tttcttatat
     6061 ggtatatcta aaatttctcc tataccaaaa catgtgtttg aaggagaatc aaatatagct
     6121 aagtcagaaa aaaataataa tccagttggt tctggtgcat tcaaatttaa agaatggaaa
     6181 aaaggtgaaa gcatagtatt tgaaaagaat gaagactatt ttggtggaga acctaaggca
     6241 gattctatag cactaaaaat aatacctaat gaagcatctc aagaagcagc tttaaataat
     6301 ggtgaaattt cacttatgaa gacatctgca gaaggatatg aaaaggcaaa atcaaactca
     6361 aacttacaaa cttatactta tagtgaagaa agattaaact acattgtatt taaccaaaat
     6421 atatcaaaca tggctaataa agaagttaga caagccttat catatgcatt aaatagaaat
     6481 gaaatgatag aatcagcgta tggaaaagaa ggctctgtac ctgctaaatc tatattagtt
     6541 ccagaagcag acttctatac agaagaaggt gttgaaggat atgaccaaaa tacaaataaa
     6601 gctaaagact tattggataa gagtggtgtt aaaattgata aattaaaaat agggtataac
     6661 acaggtagat ttgggcataa aaactatgct ttagtagctc aacaagagtt gaaaaaaatt
     6721 ggtatagaag ctgaaatagt tccttatgaa agtaaggcat tcttcaatat actatttagt
     6781 aatagcactg aatgtgatat gtatgtaaat ggttatgctt ggggattaga accaaatcca
     6841 tatagaggta tgtttgaaac tggtcaatat tgtaaccaaa ctaaatattc taatgctaaa
     6901 atagatgctt tatgggaaaa aggatttact gagttaaaca aggaaaaacg tgaagaaata
     6961 tataagcaaa tacaacagga tatatctaag gatgcaccta tttacactat agattatgaa
     7021 caaaatttaa tggcagctca aaaaaatcta aaaggaatag aagatgctaa accatctcct
     7081 gcaatattat ttgaagattg gtctaaattg tatgtagaat aattgatgta aaattttata
     7141 ttattaatta gaaaaggaga tgtctaaaaa taatattgtt ttattatttt aaaatgagac
     7201 atctctttta attaggagaa tttacttatg ttaaaattaa tattgaatag agtgaaagtt
     7261 ttaataccta tgttaatctt aatatctata ttatcatttg gacttttaga attagctcct
     7321 ggtgacccag cagatgcata tataaatcct ttaatgtctg cacaggatat tgaaaatata
     7381 agagttaata tgggactcga taaaccagta tacgtaagat atttaagctg gttgaaaaac
     7441 actttaagcg gaaatttagg tatttcttat ataaaccata tgcccgtaac agaacaaatt
     7501 atggaaaaaa tgggaaatac atttattctt atgggtactt ctttaatatt ttcaatattg
     7561 gtagctatac ctcttggaat atttttagct gtaaataaaa attcaataac tagtaaaata
     7621 tcatctattt ttaattatat aggtgtttct atcccttctt tttggatagg tatgatactc
     7681 atttcaattt tttcagttaa acttaacatt tttccatcag gaggtatgca tactattgga
     7741 aatgatagta ttggagattt agtaaaacat ctagttttgc ctgtaataac tttaggtcta
     7801 tataatactg ctatttttac aaattatgtg gaagctagtg taaatgaaca attaaaaaaa
     7861 cagtatgttg ttacagcaag agcaaaagga ttatcagaaa aagtcatact atttaaacat
     7921 gtcttgaaaa attcactgac atcattggtt acaatcctag gcatgagtat acaaaaatta
     7981 gttactggcg catttgttac agaagtagtt ttctcttggc caggtatggg aagattaatg
     8041 attgattcaa ttttttcaag agattatact gtaattatgg caataactat gttatcagca
     8101 ctatttttaa ttttaggtaa tttggttgct gatatactat acttattgat tgaccctaaa
     8161 ataaaatcta gcaagggagg attctagtta tgtttacaaa tatcttaaaa gaattaaaaa
     8221 aagacaaatc ctttgctttt gcatgtatta ctcttagtat aataatcata ttaattatag
     8281 cttcttttat attaaatata gactctaatg caattgatac tacttctaaa cttcaagaac
     8341 ctagcttaaa gcatatcttt ggtacagatg aacttgggag agattacttc tctagagctc
     8401 tttatggtgg aagaatttca cttagtgttg gtattttatc aatgcttatt tctgtagtat
     8461 ttggtactgc tgtaggtgtt attagtggat atattggggg taagctagac caattcttga
     8521 tgagactagt agatatgctt atgagtatac caagcttctt agttttgatt gtattaaata
     8581 cttatctaac acctaaaatt tccactatta tactaattat tggattctta agctggatgg
     8641 aagtttcaag aattgtacgt ggcgaaacat taaaaataaa ggaaaatgaa tactgtctag
     8701 ctgctaaagc taccggtatc aagactagaa atatactatt aagacatata gttttaaact
     8761 taaaagagac tattgtagtt gctggctcat taaatattgc taatgctatc ttaactgaat
     8821 ctgctcttag ttttttaggt cttggaatcc aattgcctct tgcatcatgg ggtaatatgc
     8881 ttcaagatgc tcaaaaatat atttttgaca agttatattt agctgtattt ccaggactac
     8941 ttatattttt aacagttatg agttttacta taataagcaa aaaattttct agtaatactt
     9001 aataaatttt atatagaaaa taaaaacagt gattatccaa agatatacac tgtttttatt
     9061 tttcgtttta aaaagcacat attttgtata atataacttt aaatactact ctagcattta
     9121 tgtaaaacct gctattgctc tcaagcacgt agatgaaaaa ctaatagttt attataataa
     9181 aaatggtttt atttatttgt tattttaata acacttttaa gagttctttt tactaaagaa
     9241 atctataata aatttaaata catgttggtc tacatggcta taacgttctt ttttaggtct
     9301 acatagtaca actttccatg taataggata attgaaaggt ttttctataa ttttgctatt
     9361 ttcaaaaaaa tcatgaactg gtgatggtag aatggttata aaatcagaat ttgtagtcga
     9421 tagcaacaaa aaatcccatg aaccagacat tatatatctc tttagtttaa cattttcatc
     9481 agtaaatttt tgcattagct tatgatgtat cataaaagta ggattaaata ttgcaagtaa
     9541 ctgctcattc aaatcgttcc aatctatctt atccttctta gcaagtggat gatttatatt
     9601 taaaaatgct gtaagttcat cttcttgaag cacatgttca gtcactacac ttttatcaat
     9661 tttatgtgga tgtaatagca ctgcataatc taattcttgt aagataagca ttctacttaa
     9721 atcatatgct cccctctcta ctatgtcaaa ttctatatct ggattatttg caactaactg
     9781 tgcaactaca tctgaaaata ctattccaag tataagtgga ggtattccta ttctaatttt
     9841 tcctttaaat tgaactgaat cttctcttaa atcttccatc atatttttgt attcatttat
     9901 aatatgttct gcatttacat aaaatctttc ccctgctggt gttaacccat ttaacctacc
     9961 tttatacctt tcaaataaat atacatcttc actttcttca aaatttttta taatttgact
    10021 tagtgctggt tgagatatat gaagtgtctt agatgctatt gataaattaa aaccagattt
    10081 cactatttca acaaaatatt ttaactgttt tatatccatt ttaacccccc ttccccaaaa
    10141 gtataattta gattatattt tttcttataa tgatatttta taggaaaatc aacataattt
    10201 cattaatatt ttaacaaaat atttttctaa atctgctcta aatttttgtc ctataaattt
    10261 ttcttataca ctatataaaa aattagactt agacaaaacg cttttttcga cgtaatctat
    10321 aattagaaac aggaaaacct aaggcattgg gaataaaaat aattgaaata cttaaaaaca
    10381 agcttgtatt agaagtactt tattatagtg ttccatattt ttcttattca gaaaattttt
    10441 aattatcatt caagaaaggt aactattaga tatgaaagac gtagtaatcg tatcagcagt
    10501 cagaacaccc attggaaatt ttggaggtgt atttaaaaat acttcagctg ttcaacttgg
    10561 aactatcact gttaaggaat caatttcacg tataggactt aatttatctg aaataaatga
    10621 agtaataatt ggaaatgtac ttcaagcagg acttggacag aatgttgcta gacaaattgc
    10681 tataaatgca ggtatcccaa attctgtccc aagttataca ataaataaat tatgtggttc
    10741 agggcttaaa agtgttcagc tagctgctca atcaattgca tctggtgaaa atgatgtagt
    10801 cattgcaggt ggcactgaaa atatgagcca agctccttat attgttccaa ctgcccgttt
    10861 tggtaataaa atgggcgaca taaccatggt agacagtatg ctaacagatg gtcttattga
    10921 tgctttcaat caatatcaca tgggaataac agcagaaaat atcgcaacta aatttgaatt
    10981 tactagggaa atgcaagata aattagcatt agaaagtcaa aataaggctg agaaggctat
    11041 aaaaaacaag cgttttaaag aggaaatcat tccagtgaat atcttaatac gtcgtggaaa
    11101 aatggaaact attgacacag atgaatatcc taaattaggt atgacatttg aaggtttgtc
    11161 taaacttaaa cctgcattca aaaaagatgg tactgtaaca gctggaaatg catctggtat
    11221 taatgatggt gcagccatgc taatactaat gagcaaacaa aaagcagacg aactaggaat
    11281 taaaccactt gttaaaataa aatcttatgc ttcagctggt gttgaacctg aaatcatggg
    11341 tacaggtcca atccctgcaa ctagaaaagc actgaaaaaa gctgggctta gtatcaatga
    11401 tatagactta atagaagcaa acgaagcatt tgctgcccaa gcattagctg ttaaaaatga
    11461 acttcaaata gattcatcaa agttaaatgt taatggtggt gcaatagcat taggacatcc
    11521 aataggagca agtggagcta gaatacttgt taccttaatt tatgaaatgc aaaaacgtaa
    11581 agttaaaact ggacttgcaa ccctttgtat tggtggtggt caaggaatat caatggtggt
    11641 ttcacgatag gaggaaatat gaataaaata gttagtattg atgaagctct atctcatgta
    11701 aaagatggag atacaataat ggttggtgga ttcatggcca atggttcacc agaaaattta
    11761 attgattatt tatgtagtaa aaatataaaa gatttgacac taatttgcaa tgatactggc
    11821 tttattgaca aaggagttgg caaaatggtt gtaaataagc aatttaaaaa gataattgct
    11881 tctcatgtag gattaaataa agaaactgga agacaaatga acaacaatga aacagatgtt
    11941 gagttagttc ctcagggaac tcttgctgaa caaatacgtg cagctggtta tggtctagga
    12001 ggtatactca caccgactgg tattggtact cttgttgagg agggaaagca aaaaattgtt
    12061 gtcgatagca aagaatacct tcttgaaaaa ccaatttatg cagatgttgc tcttctattt
    12121 gcatcaaagg tcgataaagc aggtaatcta gtttataaag gctctatgaa caattttaat
    12181 aatttaatgg catctgctgc caaaactaca atagttgaag cagatgaaat cgttgaaatt
    12241 ggcgatattg acccaaatga agttaataca ccaggtattt ttgtaaatta catcgttgaa
    12301 ggaggtaatt tataatggat aaattagaaa tgcaagaata tattgcaaat agagtatcta
    12361 aagaattaaa agatggagct gttgtaaacc ttggaatagg tctgccaacc aaagtagcaa
    12421 attatattcc agatgatgta aacgttatct tgcaatctga aaatggattt ttaggtttag
    12481 gtgcagctga agatggaaat tcaaatgatg aaactattgt aaatgcaggt ggtcaaccag
    12541 ttactatact gcctggagga tgtttctttg atagtgctac ttcttttgga ataatcagag
    12601 gaggtcatgt tgatataact gttcttggtg cacttcaggt tgacaaatat ggcaacatag
    12661 caaattatat gattccaggt aagatggttc caggcatggg tggagctatg gacttggtga
    12721 ctggagctaa aaaagttatt gtagcaatgg aacatacttc aaaaggttct gcaaaaatat
    12781 taaataattg cactcttcca cttactgcaa caaatgctgt agatttgata gtcactgaaa
    12841 tgggggttat ggaagtaaca tcagatggaa ttttacttaa agaaataaat ccagctttca
    12901 cattggatga tgttatttct tctactgaag cacctttgat attgtcggat tcattaaatg
    12961 gaaacatatc tgttgtttaa aataattaaa actagaaaat aaaaatgtct tagatgtatt
    13021 agatataaat ttatattact taaaatatta atataaaaat aaaataaaat tggaggaaat
    13081 gaaaatgctt aaagataaag ttgtattcgt aacaggagct gcaagtggaa taggtaaaca
    13141 aatagctgaa tcattcttaa aaaatggtgc taaagtaatg ttctctgata taaatcaaga
    13201 agctttaaat aagaccacaa cagagttaca acaagaaggt tatgattgca tgaatgtaaa
    13261 gtgtgatgta actaaggaag aagaaattaa ccaagctata gacaaaactg ttgaaaaata
    13321 tggcagatta gacatattaa taaacaatgc tggacttcaa cacgtttcta tgatagaaga
    13381 tttcccaaca gataaatttg aatttatgat aaaaataatg ctaacagcac cttttatagc
    13441 taccaaaaaa gcatttccta taatgaaaaa acaaggcttt ggtcgtataa taaatatggc
    13501 atcaatcaat ggtgtaatag gatttgctgg aaaggctgct tataactcag caaaacatgg
    13561 acttattgga ctaacaaaag ttgctgctct tgaagctgct gattctggta taacagttaa
    13621 tgctttgtgc cctggatatg tagatactcc acttgtcaga ggtcagttta atgacctttc
    13681 aaaaactcgt aatataccat tagaaaatgt cttagaggag gttttatacc cacttgtgcc
    13741 acaaaaacgc ctattagatg ttcaagaaat tgccgactat gctatgttcc tagcaagtga
    13801 taatgctaag ggtattacag gacaagcttg cttactagat ggaggatata ctgcacaata
    13861 gttattatat ttagggcttg aagaacttat tcttcaggtc cttctactaa ggaggaatat
    13921 ttatgagtat tatcgcacca attggtataa ttattggtat catagctatt atatattttt
    13981 cagttaaaga aattcatata actatagctg ctccaatagc aactttaata gttgttctct
    14041 taaataaaat ggatgttgta acatcaatgc taggtgctga caaaaacaat tatatggggg
    14101 cacttggaaa ctatataatg agttattttg caatattctt attaggttca atacttgcta
    14161 aatttatgga agaaagtgga gctactgtat caatagctga ttttatttta aataaatttg
    14221 gttctaatca tccttataga gttttagttg ctatatttat agtaagtttt atattaacat
    14281 atggtggtat aagcttattt gtagttatgt ttgcagttat tccacttgca agaactctat
    14341 ttaaaaagct tgatatttct tggaacctta ttcagatacc actttggctt ggtattgcaa
    14401 caattactat gactataatt cctggaacac cagccattca aaatgttata cctattcaat
    14461 atctaaatac atctcttact gcagctacta taccaagtgt tgcaggtagt ataggttgtg
    14521 ctacgtttgg gcttgtctac atgaaatatg ctctaaacaa aagcattaga aaaggtgaaa
    14581 cttatgcaac ttacacttca gaattagaag aagaatcttt agctagggaa ttaccaggat
    14641 tttttgcaag tatttttcct ctattagcac tcgtaattat agctataact ggaagtactt
    14701 ttggttcaga atttatgaaa aaaaatgtaa tttacatagc tttaatagtt gcaattcttt
    14761 tatctgcttt tttattccat aaatttttat catctaagat acagacattg agtataggtg
    14821 ctagtggttc tataggtcct atatttgcta catcttctgc agttgcattt ggagcagtaa
    14881 taatggctgc acctggtttt gatgtattta gcaagcttat aatgtcaata ccaggtagtc
    14941 cacttataag tcttactgtt ttgacttcaa caatgtcagc aataacaggg tcatcttctg
    15001 gtgcattagg aatagtaatg ccaaactttg cagattatta tttaagtact ggtctaaatc
    15061 cagaattaat tcaccgtgtt gctacaatag cctcaaatat actaactata gttccacaaa
    15121 gtggtgtttt tcttacattc ctaagcctct ctaagttaac acataaaact ggatttaaag
    15181 aaactttcat tgtagttgca gttggttcac taatagcaga aatcattgta attacattag
    15241 gattattaat gtattaatat aaaataaatc agaaattaca atataaaagg gaatgtctca
    15301 aaatgaattt tttagttaat gagacattct tttttgtatg aaatcacata tacatttatt
    15361 atttcaacaa tggaagtgag gctttctcta ttttgatgta gaccctttta ttccttaaag
    15421 atttaaataa ttataatttg ttactaatat taaagcattt atttaataaa tttaactaaa
    15481 taaaaaaaaa atttatcata actataaatt tgcattatag aattattata aaaatattat
    15541 ttgctctaaa taagtaattc catactatac acacttctaa tacaaactgt caaacactct
    15601 gaaatatgcc taattattcc atatatgtct tttttacata tctttacagt aaaaaaatac
    15661 ttgaaattgt gttagtatag attcacgggt accccctaca ctaaaggtta caaattagta
    15721 acatttttag aatttttaga ttgagaggtg tttagtaatg agaaaaaata taccaacaaa
    15781 attaaaaact atattagctt taggagtgtt aaccttaaca acagcttcca gtgctgttaa
    15841 tgcaagtgct ttatcacttg aaaataatgt aaatacaagt aacaaaaatt tagtatacag
    15901 taatactagg tacaaatttt ctgcaaaacc aaattgttct ataaaaccag aacttaagcc
    15961 tgataaagac aactcttgca aagataagaa tcatagtgac aaaaattgca acaatactaa
    16021 taaacctgat aacaataaac cagagtctaa taaacctgat gacaataata attcaggttc
    16081 tactgataaa cctgacaaca ataaaccaga gtctaataag cctgatgata ataataattc
    16141 aggttctact gataaacctg acaacaataa accagagtct aataagcctg atgacaataa
    16201 taattcagac tctactgata aacctgataa caataaacca gagtctaata aacctaatga
    16261 caataataat tcagactcta ctgataagcc taatgataat aacaattcag gctctacaag
    16321 tgaaaatttt tcagcttacc aaaaagaagt tgtcgactta gtgaatatag aaagatctaa
    16381 agctggatta aatcctctta ctcttgattc ttctatatca aacgttgcta caaaaaaatc
    16441 tcaagatatg attgataaca actatttttc acacaattct ccaacttatg gttctccatt
    16501 tgatatgttg aaaaaatttg gaataagcta taaaacagca ggtgaaaata tagctatggg
    16561 tcaaaagaca ccaaaagaag ttgtaaatgc gtggatgaat tctgaaggtc acagaaaaaa
    16621 tataatgaat ccaaatttca gtaaaatagg tgttggagta gcacaaaaaa gtggtggttc
    16681 aatttattgg acacaaatct ttgtaggata aaatactctt tttccataca tatcaaaagg
    16741 gtatctctaa gtgataaaaa acacacaaat gagatactct tttgtatcta tatttaaaat
    16801 tattaaatac aaacatatat agtataaata gtattgttct ctaccagtga ttttgatgta
    16861 cagaacaata tataaaaagt attggcaata tgaaaatgga aaacttaaaa aaggtattta
    16921 gcagataaaa acaaaatctt aatatcaaaa atgaaaaaga gtcttgtctt aaaatagaga
    16981 caagaccctt tttcattgtt gatatgatta aaatatattt tattttatat aaaaaagagt
    17041 gcctcatgaa ctaaaaagtt cactttgaga cactcttttt atcaattcta tataagcatt
    17101 ataatacttt aatattattc agctaatttt ttgtatcctt catatctttc tttagcatca
    17161 gcttcagctt tagcaaataa tccatcagct tcctctggga attgtttagc tatagcagaa
    17221 tatcttactt gtgcaagtat gaaatctttg aagcttgcag ttggttcttt tgaatctaaa
    17281 gtgaatgggt ttttaccttc agcttttact tctggattga atctatataa atgccaatat
    17341 ccgcaagcaa cagcttgtgc ttcgttagct acagttcttc ccataccttc ttttaatcca
    17401 tggtttatac atggagcata agctattatt agagatggtc catcatgttt ttcagcttct
    17461 agaacagctt tgataaattg gtttttatca gcacccatag caacttgagc tacatatacg
    17521 tttccgtatg tcatagccat cattcctaaa tctttctttc tagatttttt acctgaagca
    17581 gcaaacttag ccatagcagc agctggagta gctttagaag cttgacctcc tgtatttgaa
    17641 tatacttctg tatcaaatac aagcacattt acatcttcac cagatgcaag aacatggtct
    17701 aatccaccat aaccgatgtc atatgcccaa ccgtctccac ctagtatcca ttgagatctc
    17761 ttaactagat aatcttttct atcttttata gcttttacta attctttagc tttttcatct
    17821 gtacaatcac aagctggttt ttctaataat tcaacaacct tagcacttgc tactttagaa
    17881 gcttctccat catttctaga atctaaccac tctgtaaata ctgctttagc atcttcacag
    17941 atatccatag aaactagctc tgtcatagca tctactattt tgtttcttat ttgcttaaca
    18001 gctgtgtaca taccaaatcc atattcagca ttatcctcga ataaagagtt tgcccaagat
    18061 ggacctttac cttcatggtt acaagtataa ggaattgatg gtgcagaagc tccccatata
    18121 gaagaacatc cagtagcatt agctatcatc attctatctc catataattg tgtaactact
    18181 ttaatgtatg cagtttctcc acatccagca catgctcctg agaactccat taatggttgt
    18241 ctaaattgac ttcctttaac agtatttgca ggcataacgt caacttttgg agctacttca
    18301 tcaggattta ctgcaaatgc ccaattatct aactctgtat caagttgagt atctattggc
    18361 ttcataacta aagctttttc tttagctgga catatatcag cacagtttcc acatcctgta
    18421 cagtcaagtg gactaacttg aattctatat tttaatcctt ctaatccttt acctaatgct
    18481 tttttagctt caaatccttc tggagcatta gctagttctt cttcagtaac taatactggt
    18541 cttatacatg catgaggaca tataaaagaa cattggttac attgaataca gttatctatt
    18601 atccattcag gaacatcaac agctatacca cgtttttcat aagctgcagt accacaaggg
    18661 aatgttccat cttctatacc attgaatgta cttactggta agttgtttcc ttcttgtgca
    18721 gtcattggtc ttagtatatt ttttataaat tctggttctt taacttctcc tttatctgca
    18781 tcaacagcat tttcccaaga agctggaact tcaactttaa ctaaagcatt tattcctgca
    18841 tcaacagcag catagttcat gttaactatt ttttcacctt ttttaccata agctttaact
    18901 atagaatctt ttaagtattt aacagcatct tcttctggta ttatttcagc taatttaaag
    18961 aatgcagatt gcattatcat gtttattctg tttccaagac ctatttcttg tcctagttta
    19021 acagcgttaa ctgtatagaa ttttatatca tttttagcta tatattgctt catatgagct
    19081 ggtaaatgtt cttcaagtcc tgcttggtcc catatagtgt taagtacaaa tgtaccacct
    19141 tttttaagac cttttaataa atcatattga taaacatatg attgtttatg acaggcaata
    19201 tagtcagctt catctattaa ataagtcgct cttattggac tatcaccaaa tcttaagtga
    19261 gacatagtta ctccaccaga ttttttagag tcataatcaa agtaagcttg agcgtatttt
    19321 tcagtatggt caccgattat ttttatagct tgcttattag ctccaacagt accatcagaa
    19381 cctaatcccc agaacttaca tcttatagtt cctttagaag ctatttttat tggttcagat
    19441 ggagttaaag aagtattagt tacatcatca actatactta ctgtaaatcc atttttaggt
    19501 tgttctgaaa ctaagttatc aaaaacagtt tttatatctg aaggagtagt gtcttttgaa
    19561 cctaatccgt atcttccacc tattatcata ggagcatttt cttttccata gaatacatca
    19621 cgtacatcta agtataatgg ttcaccagtt gaacctggct ctttagttct atcaagtaca
    19681 catattcttt caacagtaga tggcatagca tctaagaaat gtttcattga gaatggtcta
    19741 tataaatgaa ctttaactaa accatatttt tctcccttag catttaagta atctatagtt
    19801 tcttctattg tttctgttac agaacccata gccactaata cgtatttagc atcttctgct
    19861 ccgtagtaat caaataaact atgttttcta cctgttaaat tactcatttt ttccatgtat
    19921 ttttcaacta tacctactat attttgatag tatttattag aagcttctct agtttggaag
    19981 tatatatctg ggttttgagc agtaccacga gcaactggat gatttggaga taaagcatta
    20041 tctctaaatg cttgaactgc ttcaaagttt aataaacttg cataatcttc attttctaat
    20101 aattcaactt tttggatttc atgtgaagtt ctgaatccat caaagaaatg tataaatggt
    20161 aatctacctt ctattgcagc taaatgtgca acaggtgcta tatcagctac ttcttgaact
    20221 gaaccagaag ctaataatac acatccagtt tgtctagcag ccataacgtc ttggtgatcc
    20281 ccaaatattg ataaagcttg agatgctaaa gcacgagcac ttacatggaa tactcctggt
    20341 aataactctc ctgcaacttt atacatgtta ggtatcatta ataataaacc ttgagatgct
    20401 gtaaaagtag aagttaaagc tcctccttgt aaagaaccat ggaatgttcc tgatgcacct
    20461 gcttctgact gcatttcaac aacattaact gtttgtccaa atatattttt tcttccttgt
    20521 gatgcccact catcaactac ttcagccata gttgaagatg gtgtgattgg atatatagct
    20581 gctacatctg taaacgcata ggcaacatga gctgcagctg tatttccatc aagtgttttc
    20641 ataaacttag ccataatatg tatcctcctt gatagctaca attgtacttg tttataacaa
    20701 gtctattttt ttatatttac atataattta cacaacaaaa atataaacac acatatagtc
    20761 gcgcaaatac tctatcttct aatataatac tacagattag ataaaattcc tttgaaaatt
    20821 gtaa
//
LOCUS       DAFNNB010000060        20823 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0060, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000060 DAFNNB010000000
VERSION     DAFNNB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20823
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0060"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            362..1384
                     /locus_tag="L3M60_001864"
     CDS             362..1384
                     /locus_tag="L3M60_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6226533.1"
                     /translation="MKNLLEIQKKLIPQAIELMERRYSILRQISLSEPIGRRTLSNVL
                     EISERIIRSETEFLKEQGLIDVAVSGMTITKEGSDLLDKLKDIMSDIMGLSKLQDRVR
                     EKLGIKRVILVPGSCDENGSLLKDVTRYGCEYFLGILKDGDIVSITGGSTMLEFSNVI
                     KTDKRYPNSTIVPARGSMGTDVEMQSNNIVASISKKLHSNCKLLHIPDELDESAMKTL
                     SQVPEIKTTLEYIKRTDILVFSIGRADVMAKRRKLPEDKVDEILSKNAVGEAFGYYFN
                     KDGEIVYKLNTVGIDLETVKQVKETIAIFAGTEKVEALLAISNMHKNMVLVTDEESAY
                     KILSSL"
     gene            1444..2451
                     /gene="gap"
                     /locus_tag="L3M60_001865"
     CDS             1444..2451
                     /gene="gap"
                     /locus_tag="L3M60_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBN6226534.1"
                     /translation="MVKVAINGFGRIGRLALRKMMEQQDKFEVVAINDLTDAKMLAHL
                     FKYDTAQGRFNGEIEVKEGAFVVNGKEIKVTAERNPADLPWAELGVDIVLECTGFFTS
                     KDKAEAHIQAGAKKVVISAPATGDLKTIVFNTNSDILDGSETVISGASCTTNCLAPMA
                     KVLNDKYGIEKGLMTTIHAYTNDQNTLDGPHPKGDLRRARAAAGNIVPNTTGAAKAIG
                     LVIPSLKGKLDGAAQRVPVVTGSITELVCTLGKNVTVEEINAAMKEASNESFGYTEEM
                     LVSSDIIGISYGSLFDATQTKVMEVDGKQLVKVVSWYDNEMSYTSQLIRTLGYFAQLA
                     K"
     gene            2673..3875
                     /locus_tag="L3M60_001866"
     CDS             2673..3875
                     /locus_tag="L3M60_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBN6226535.1"
                     /translation="MSMLNKKTIEDIDVCGKKVLVRCDFNVPLQDGVITDENRLNGAL
                     PTIQYLISKGAKVILCSHLGKPKGEAKPELSLAPVAKRLSEMLGKEVVFAADDNVVGE
                     NAKKATEEMENGDVVLLENTRYRKEETKNEENFSKELASLAEIFVNDAFGTAHRAHCS
                     TVGAGEFLQERVCGYLIQKELKFLGEAVANPVRPFTAILGGAKVSDKLAVINELLEKV
                     DNLIIGGGMAYTFLKAQGYEVGTSLLEIDKVEYAKEMMEKAKNKGVNLLLPVDVVMAD
                     HFAPDATPIVTEDANVKEGYMGLDMGPKTIANFVKTIKESKTIVWNGPMGVFEFENFA
                     NGTLSVARAMAELTDATTVIGGGDSAAAVNQLGFGDKMTHVSTGGGASLEFLEGKELP
                     GIAALDNK"
     gene            3901..4644
                     /locus_tag="L3M60_001867"
     CDS             3901..4644
                     /locus_tag="L3M60_001867"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBN6226536.1"
                     /translation="MRKPIIAGNWKMHKTIKEALEFVNEVKDKVNSDKVEAVICAPFT
                     LLKDLKEATKGTNIKIGAQNMHFEEKGAFTGEVSPLMLKEIDMDYVVIGHSERRQYFN
                     ETDETVNKKVLKALEVGIDPILCVGETLEQREAGKTKDVCRVQVEKALENVLKDDLAK
                     VVVAYEPIWAIGTGKTATAEDANDVISYIREVIKGLYGELANEVRIQYGGSVKPSNVA
                     EIMGQSDIDGALVGGASLASNDYLDLVNF"
     gene            4653..6185
                     /locus_tag="L3M60_001868"
     CDS             4653..6185
                     /locus_tag="L3M60_001868"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003228330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBN6226537.1"
                     /translation="MMKKPVALIIMDGFGYNKDVKGNAIAESKTPNLDRIKKEYPNTL
                     INASGLDVGLPDGQMGNSEVGHTNIGAGRIVYQDLTRITKSIKDGDFFTNKVLCEAMD
                     NAKENSLHVMGLLSDGGVHSHIDHLKAIIKMAKDKGVQKVYVHAFTDGRDTDPQSALE
                     YAKEVQASMDEIGVGEFATVSGRYYAMDRDKRWERVELAYNAMVRGIGEKATSIEEAI
                     QNSYDDGKNDEFIMPTVIMKDDKPVGSIKENDSIIFFNFRPDRARQITRALVCEEFDG
                     FKREDIKNFFVCLTEYDITIENVHIAFGPQSLANTLGEYLAKNGKTQLRAAETEKYAH
                     VTFFFNGGVEEPNKGEERLLIPSPKVATYDLKPEMSAYELTDKALDKLGEDKFDFIVL
                     NFANPDMVGHTGSIEAAVKAVETVDTCVGKLIDKIVELGGSAIITADHGNAEYMLDPE
                     TGKTVTAHSINPVPFIVVGQEYENAKLLDGGRLSDIAPTVLDMMKLEKPEEMTGHSLI
                     SK"
     gene            6773..8065
                     /gene="eno"
                     /locus_tag="L3M60_001869"
     CDS             6773..8065
                     /gene="eno"
                     /locus_tag="L3M60_001869"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBN6226538.1"
                     /translation="MSVIELVYAREVLDSRGNPTVEVEVVLEDGAMGRAIVPSGASTG
                     AFEAVELRDGDKGRYLGKGVETAVANVNEIIAPEIEGMDAFDQPAIDAIMIELDGTPN
                     KGKLGANAILGVSMAVARAAADEIGLPLFQYLGGVNAKQLPVPMMNILNGGEHADNNV
                     DVQEFMILPVGACCFKEGLRMGAEVFHSLKKVLGEKGLACGVGDEGGFAPNLGSNREA
                     LELIVEAITKAGYKPGEDVMLGLDVAATEMYNKETKKYVLAGEGKELTAAEMVALYED
                     WSNNFPIITIEDGLDEEDWDGWKLLTEKLGNKLQLVGDDLFVTNTERLEKGIENGVAN
                     SILVKVNQIGTITETLDAIEMAKRAGYTAVISHRSGETEDSTIADLAVAVNAGQIKTG
                     APSRTDRVAKYNQLLRIEEMVGEQARYCGLKSFYNLKK"
     gene            complement(8166..8596)
                     /locus_tag="L3M60_001870"
                     /pseudo
     CDS             complement(8166..8596)
                     /locus_tag="L3M60_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429569.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            8662..8883
                     /locus_tag="L3M60_001871"
     CDS             8662..8883
                     /locus_tag="L3M60_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6226539.1"
                     /translation="MVFYEFRYFRPAFWGTPVAKKGLQFCIDGLRNLGYPYITIWVLK
                     DNIRVIKFYKKMVLLVMILRKIYVLEKNY"
     gene            9026..9250
                     /gene="secG"
                     /locus_tag="L3M60_001872"
     CDS             9026..9250
                     /gene="secG"
                     /locus_tag="L3M60_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBN6226540.1"
                     /translation="MSNILMGVQVVLGILLVISIMPQDSKNALPSEFGGEGSQAYFKP
                     KGKQAFLARVTKITAVLFFINALALLIVKK"
     gene            complement(9610..10548)
                     /locus_tag="L3M60_001873"
     CDS             complement(9610..10548)
                     /locus_tag="L3M60_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBN6226541.1"
                     /translation="MENLILSINVVLPLFLTMSLGYVLKKLGLFDVHTLNKMNNVCFK
                     SFLPLLLFFNIYNTDLDGSLNFKLMAVAVIIIFSIFLILVFIIPKIEKNNKTRGALIQ
                     GIFRSNFVIFGIPVTISLFGDKSIGVTSLLIAVIVPMFNVLSVIILEIFRGGKVNIKH
                     MIKSVLTNPLIIASFIGLTMLILNINIPTFLHKTIGDISKIATPLSLIILGGSFSFSA
                     IKGHIKPIIFGVCGKLILVPAIFLPISIFLGFRNVELASLMIMLSAPTAVSSFTMSQQ
                     MDSDSELAGQIVVFTSAFSIITVFLIIFILKQNNLI"
     gene            complement(10536..10934)
                     /locus_tag="L3M60_001874"
     CDS             complement(10536..10934)
                     /locus_tag="L3M60_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN6226542.1"
                     /translation="MTKKIKHVLITTNNIENYINFYKKFGFSFKETSEKYKFNAGNVK
                     INLNIKGHEFLPQGENVQTISTNLCFKIDTNIDEPKKYLEQNEIQIKRVIVECTEVFS
                     NMKSICFKKTDVNLIELSNHRILESKPWKI"
     gene            complement(11359..11856)
                     /locus_tag="L3M60_001875"
     CDS             complement(11359..11856)
                     /locus_tag="L3M60_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HBN6226543.1"
                     /translation="MKVTSTGIVNGVIEDKYGKRGTQFNEGGMPTYSLPLKFEDAPKN
                     TVSFAVFLEDKDSVPVCGFAWIHWLAVNITKDELKENESVTATDFVQGTTSWHGKLGG
                     LDRLDASVYGGMAPPDRPHVYEIHVFALDTILDLEKGFYMNEFFKAIEGHVLDTFTLK
                     GSYSN"
     gene            complement(11962..12324)
                     /locus_tag="L3M60_001876"
     CDS             complement(11962..12324)
                     /locus_tag="L3M60_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226544.1"
                     /translation="MKLRDEYTCPLELTHDMIKGKWKSIILWRLRIGPTSLSKLEKEI
                     NGINQKMLLEQLKELIEFGLVDKKTYKGYPLKVEYSLTPIRGKQILDALAIMQDIGIN
                     FMLDNGMEDILKEKGVLK"
     gene            complement(12342..12956)
                     /locus_tag="L3M60_001877"
     CDS             complement(12342..12956)
                     /locus_tag="L3M60_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBN6226545.1"
                     /translation="MEEVIAKIITIENGFKEIENIAKKIVKNNDSKKCYYLSIYLYRS
                     EYYQVRELAVFILGFISVTISEALLFLKDNVSKDENWRVQEILAKSFDYYCSEVGYEK
                     ALPVIKEWLNSDSPNIKRAVTEGLRIWTSRDYFKSNPDVAISLLSSLKEDDSEYLRKS
                     LGNALRDISKKYPDLIKKELDSWDISNKKVEFVYKLASKRILAK"
     gene            13557..15692
                     /gene="rnr"
                     /locus_tag="L3M60_001878"
     CDS             13557..15692
                     /gene="rnr"
                     /locus_tag="L3M60_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBN6226546.1"
                     /translation="MIPGLKESLLGLINESAYNPLKKEELAEIFDIHSSEMPMFYNFL
                     NELEEDGYICFTKKGKIISPNQMGYFVGKFVSHKKGFGFVESDVEYTQDLFISSDNIN
                     GAMHNDRVMAEIVVPATEEKRAEGRIVKVIKREITRIVGTFQSSKTFGFVTPDNKKFT
                     KDIYIPKKHFSGAVDNDKVVCEITVWPQEDRKPEGKIIEILGQKGERGVEIDSIIREH
                     GLPEEFPKKVLQEAEAVAVEIPQEEIKRRRDLRDLNIFTIDGDDAKDLDDAISIEVLS
                     NGNFKLGVHIADVTHYVREKNKLDKEALKRATSVYLVDKVIPMLPKTLSNGVCSLNPF
                     EDKLTLSIFMEIDHKGNVVKHEICESVINSKARMTYTEVSDILEKDDEKLKKTFEHVV
                     EDFKNAEVLARILMSRREKRGAIDFNFPEAKIILNGKGEVVDIKPYERRISNKIIEEF
                     MLISNETIAEHFYWMGIPFVYRIHETPSLEKMEELSKFISTFGYIIKGDKEEVHPKAL
                     QGIIEKIKGKKEEGAISTIMLRSLKQAKYSPECSGHFGLAAKFYCHFTSPIRRYPDLQ
                     IHRIIKESLNNKISGKRQEQLTTIVDYASVQSSEKERKAELAERDVHDFYKALYMKDK
                     VGQEFEGVVSSVTSFGIFVELDNTIEGLIRLANMNDDYYVYDESSYSVLGERTKKSYK
                     IGDLVKIKVESVNVDFKEIDFEIVEKIEE"
     gene            16831..17322
                     /locus_tag="L3M60_001879"
     CDS             16831..17322
                     /locus_tag="L3M60_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HBN6226547.1"
                     /translation="MGMIGCYTKISEENVFKLQQAEENLQDFVFEDSNENSTINIDKA
                     WHAIHFTLTGCPFGGDEDNIFSKLVLSGNILMEIDGEFPVMLITANDVKKLSKAMNSL
                     EEQEFRKRFNINEMLENNIYPVMNDESEDDFFEYVWANLIELKRFIQEASDDGQAVIF
                     FIM"
     gene            17370..17522
                     /locus_tag="L3M60_001880"
     CDS             17370..17522
                     /locus_tag="L3M60_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226548.1"
                     /translation="MKTIEKYIKEMIGESISEINSLWKTGESIKINYSKVSSERFEEI
                     IQRVTP"
     gene            17595..18006
                     /locus_tag="L3M60_001881"
                     /pseudo
     CDS             17595..18006
                     /locus_tag="L3M60_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903589.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(18281..18745)
                     /locus_tag="L3M60_001882"
     CDS             complement(18281..18745)
                     /locus_tag="L3M60_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6226549.1"
                     /translation="MNLNKVKFTEEHAIEVINWKYEGKYSIYNLPPWEEIKKKNFSLA
                     KEDRRKNFISFINDNKELIGFINLLDEGSSVFFGIGIKPEYCGMGIGKEIISLGLQEC
                     RNKYSTKPIVLNVRTWNMRAVKCYESQGFKIVETKRQKTYLGDGEFFVMRYQ"
     gene            complement(19757..20584)
                     /locus_tag="L3M60_001883"
     CDS             complement(19757..20584)
                     /locus_tag="L3M60_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBN6226550.1"
                     /translation="MDLSKKVKILENASDGKSEFEILEFEKLEGASSPMMAMGLYFAR
                     EAGLKARQIKIKLNNSSIKTEAGALYYYKGNISSETKIGGVGGLFKKGISGALTNESM
                     MKPVYKGTGEVYLEPSFRHYLFMSLDNDSIIVDKGLFYCCSDTIEVKAFTQKNFSSAL
                     LGGEGFFQIELSGTGVIVLECVVPQSEIVEYELKNGEELKVDGNFAIARTSGVNFSVT
                     KSDKSLIGSALNGEGFLNTFTGNGTVWLAPTQVMYEKLSFGTMPTNDNMNNKVSKQK"
BASE COUNT         8122 a         2467 c         3347 g         6887 t
ORIGIN      
        1 tattattaaa tttaataatc acaaaaggaa aacacatagg gtataaataa aaattttagc
       61 tagacagaag taaacttagc tggaaataaa agtaactaga aataaaatta tttatagtaa
      121 taaatattat aacagaaaat attaaaataa aaaaatatat ataaaaagcc ttatataaaa
      181 agaaaaaata ctgtgatttg aaaatggtgg agaaatattt tataaaataa aaaaataaaa
      241 aagtttttga aaaactattg aaatctatac gcaaatccta taaaataaaa atatcttgtg
      301 ggacttaaat aaacactagg gacgaaaaaa gtcccgaaag gaaagcaata aggagtatgt
      361 tatgaagaac ttattagaaa ttcaaaagaa attgattcca caggcaattg aacttatgga
      421 aagaagatac tctattttaa gacaaatatc tttaagtgaa ccaataggta ggagaacatt
      481 atcaaatgta ttagagatta gtgaaagaat aatcaggtca gaaacagagt ttttaaaaga
      541 acaaggactt atagacgtag ctgtatctgg gatgactatc actaaagaag gttctgattt
      601 gttagataaa cttaaggaca tcatgagtga catcatggga ttgtctaagt tacaagatag
      661 agtcagagaa aagcttggaa taaaaagggt aatactagtt cctggaagct gtgatgaaaa
      721 tggaagttta ttaaaagatg taactagata tggatgcgag tatttcttag gtattttaaa
      781 agatggagat atcgtttcaa taacaggagg aagcactatg ttggagtttt caaacgttat
      841 taaaactgat aagagatacc caaattctac aatagttcca gctagaggaa gcatgggaac
      901 tgatgtagag atgcaatcta ataacatagt tgcttcaata agtaagaaat tacattctaa
      961 ttgcaaactt ttacatatac cagatgagtt ggatgaaagt gcaatgaaaa ctcttagcca
     1021 agtgccagag attaagacaa ctttggagta tattaaaaga acagacattt tagtttttag
     1081 tataggtaga gcagatgtca tggcaaagag aagaaaactt ccagaagaca aagttgatga
     1141 gatattatct aaaaatgcag ttggagaagc atttggatat tatttcaaca aagatggaga
     1201 aatagtttac aaactgaata ctgttggaat tgatttagaa acagtcaaac aagtaaaaga
     1261 aactattgca atctttgcag gtactgaaaa agtagaagcc ttattagcca tatcaaatat
     1321 gcataaaaac atggttcttg taacagatga agaaagtgct tataagatat tgtcatcatt
     1381 ataaaagtca agacctagca cagagctaga aaacatacat ttactttata ggaggtagcg
     1441 aaaatggtta aagtagctat aaatggattt ggaagaatag gaagattagc attaagaaaa
     1501 atgatggagc aacaagataa gtttgaagtt gttgcaataa acgacttaac agacgctaaa
     1561 atgttagctc atttatttaa atatgataca gctcaaggaa gatttaacgg agaaatagaa
     1621 gttaaagaag gtgcttttgt agtaaacgga aaagaaataa aagtaacagc agaaagaaat
     1681 cctgctgacc taccatgggc tgaattagga gtagatatag tattagaatg tacaggattc
     1741 tttacatcta aagataaagc tgaagctcat atacaagcag gagctaagaa agttgttata
     1801 tctgcaccag ctactggaga tttaaaaaca atagtattca acacaaacag tgacatatta
     1861 gatggttctg aaacagttat atcaggagct tcttgtacta ctaactgttt agctccaatg
     1921 gctaaagtat taaacgataa atacggtata gaaaaaggat taatgactac tatacatgca
     1981 tacactaatg accaaaatac tttagatggt cctcatccaa aaggtgattt aagaagagct
     2041 agagctgctg ctggaaacat agttccaaac actacaggag ctgcaaaagc aatcggttta
     2101 gtaatacctt ctttaaaagg aaaattagat ggggcagcac aaagagtacc agttgtaact
     2161 ggttcaataa ctgaattagt ttgtacatta ggaaaaaatg ttacagttga agaaataaat
     2221 gcagctatga aagaagcttc aaacgaatct tttggatata ctgaagaaat gttagtatct
     2281 tctgacataa tcggaataag ctacggttca ttatttgacg ctactcaaac aaaagttatg
     2341 gaagtagatg gaaaacaatt agttaaagtt gtttcttggt atgataatga aatgtcttat
     2401 acttctcaat taataagaac tttaggatac tttgcacaat tagcaaaata gtcagttttg
     2461 agaaaataga aaagtagatt gacaaagtcc gagtttgtag tttaatacta cggactcagg
     2521 acttttttta agaaaaaata tataaaatac acctaaataa tgttacatac tatatataaa
     2581 aatatgataa tatgatatat ttaatagggt aaaagtatgt actatattat acttggcaat
     2641 aaaaaattac tttattaaaa ggagagatat ttatgtcaat gcttaacaaa aaaactatag
     2701 aagatattga tgtgtgcgga aaaaaagtat tagtaagatg tgactttaat gtgccattac
     2761 aagatggtgt aattactgat gaaaatagat tgaacggagc tttaccaact attcaatatt
     2821 taatttctaa aggggcaaaa gttatattat gctctcactt aggaaagcca aaaggagaag
     2881 ctaaaccaga attatcttta gcaccagtag cgaagagatt atctgagatg ttaggaaaag
     2941 aagttgtatt tgcagcagac gataatgttg taggagaaaa tgcaaagaaa gcaactgaag
     3001 aaatggaaaa cggagatgta gtattattag aaaatactag atatagaaaa gaagaaacta
     3061 aaaacgaaga gaatttctct aaagaactag catcacttgc tgaaatattt gtaaatgatg
     3121 catttggaac tgctcataga gcacattgct caacagttgg tgcaggtgag ttcttacaag
     3181 agagagtttg tggatattta attcaaaaag aactaaaatt cttaggagaa gctgttgcaa
     3241 accctgtaag accatttaca gctatacttg gaggagctaa agtttctgat aaattagccg
     3301 ttataaatga attattagaa aaagttgata atctaataat aggtggagga atggcttata
     3361 cattcttaaa agctcagggt tatgaagtag gtacttcttt acttgaaata gacaaagttg
     3421 aatatgctaa agaaatgatg gaaaaagcta aaaataaggg tgttaactta ttattaccag
     3481 tagacgttgt tatggcagac cactttgcac cagatgctac tccgatagta acagaagatg
     3541 ctaatgtaaa agaaggttac atgggactag atatgggacc taagactata gcaaactttg
     3601 taaaaactat aaaagaatca aaaactatag tatggaatgg accaatggga gtatttgaat
     3661 ttgaaaactt tgcaaatggt acattatcag tagctagagc tatggcagag ctaactgatg
     3721 caactactgt aattggtggt ggagatagtg cagcagcagt taatcaatta ggatttggtg
     3781 ataagatgac tcacgtatca acaggtggag gagcatcact agaattttta gaaggtaaag
     3841 aattaccagg tattgcagca ttagataata aataaactaa tatatagagg agattgtaaa
     3901 atgagaaaac ctataattgc aggaaactgg aaaatgcata aaacaataaa agaagcttta
     3961 gaatttgtaa atgaagttaa agataaagta aattctgata aagtagaagc tgttatatgt
     4021 gcaccattta ctttattaaa ggatttaaaa gaagctacta agggtacaaa tataaagata
     4081 ggtgctcaaa atatgcactt tgaagaaaaa ggagcattta caggagaagt ttcacctctt
     4141 atgttaaaag aaatagatat ggactatgtt gtaataggac attcagaaag aagacaatac
     4201 ttcaatgaaa ctgatgaaac tgtaaataag aaagttttaa aagcattaga agtaggaata
     4261 gacccaatat tatgtgttgg agaaacttta gagcaaagag aagctggaaa aactaaagac
     4321 gtttgtagag ttcaagttga aaaagcttta gaaaatgttc ttaaggatga tttagcaaaa
     4381 gtagttgttg catatgagcc aatctgggct attggaactg gtaaaactgc aactgctgaa
     4441 gatgctaatg acgttatatc ttatataaga gaagttataa aaggattata tggagaatta
     4501 gctaatgagg ttagaataca atacggtgga agtgttaaac cttcaaatgt agccgaaata
     4561 atgggtcaaa gtgatataga tggagcttta gtaggaggag ctagcctagc tagtaatgat
     4621 tacctagacc ttgttaattt ctaaggagtg aaatgatgaa aaaaccagtt gctttaatta
     4681 ttatggacgg gtttggatac aataaggatg ttaaaggaaa tgcaattgct gagtccaaaa
     4741 ctcctaattt agatagaata aaaaaagagt atccaaacac attgataaat gctagtggtc
     4801 ttgatgttgg tctacctgat ggtcagatgg gaaattcaga agttggtcac actaatatag
     4861 gtgcaggtag aatagtatat caggatttaa ctagaataac aaaatctata aaagatggtg
     4921 acttttttac taataaagtt ttatgtgaag ctatggataa tgcaaaagaa aattctcttc
     4981 atgtaatggg acttttgtct gatggaggag tacattcaca tatagaccat cttaaggcta
     5041 taatcaaaat ggcaaaagat aaaggtgtac aaaaggtgta tgttcatgca tttactgatg
     5101 gtagagatac tgaccctcaa agtgcattag aatacgccaa agaagtacaa gctagtatgg
     5161 atgaaattgg agtaggtgaa tttgctacag tttctggtag atactacgct atggatagag
     5221 ataagagatg ggaaagagta gagcttgcat acaatgctat ggttagaggt attggtgaaa
     5281 aagcaacttc tattgaagaa gctattcaaa actcttatga tgatggaaaa aatgatgagt
     5341 ttataatgcc aacagttatt atgaaagatg ataaaccagt tggaagtata aaggaaaatg
     5401 attcaattat attcttcaac tttagaccag atagagctag acaaataact agagctttag
     5461 tttgtgaaga gtttgatgga tttaaaagag aagatattaa aaacttcttt gtatgcttaa
     5521 cagagtacga tataactata gagaatgtgc atatagcatt tggacctcaa tctttagcca
     5581 acactctagg tgagtatcta gctaaaaatg gaaaaactca acttagagct gctgaaactg
     5641 aaaagtatgc acatgtaact ttcttcttca atggtggagt agaagagcca aataaaggtg
     5701 aagaaagact attaatacct tctccaaagg ttgcaactta tgatttaaaa ccagaaatgt
     5761 cagcatatga attgacagat aaagctttag ataaattagg agaagataag tttgacttta
     5821 tagtattaaa ctttgccaat cctgatatgg taggtcacac aggaagtata gaagcagctg
     5881 taaaggctgt tgaaactgta gacacttgtg taggaaaatt gatagataaa atagtagagt
     5941 taggtggaag tgcaataata actgctgacc acggaaatgc tgaatatatg ttagacccag
     6001 aaacaggaaa aactgtaaca gctcactcta ttaatccagt accatttatt gtagttggtc
     6061 aagaatatga aaatgcaaag ttattggatg gaggaagatt atctgatata gcaccaactg
     6121 ttttagatat gatgaagtta gaaaagccag aagaaatgac tggtcattca cttatttcaa
     6181 aatagtcgat tatgataagt ataaaataag atataagcta caatataaat ttaaaaataa
     6241 cacagtttag gtttattttg taaggtctag attttatgat ataagattta gcttttgtaa
     6301 ggtctggata ttgtaaggtg tagatgaaag tatattttat aatatttaca atatagacaa
     6361 tttataaaat aaaaaattat aatatagata atgatataaa taataatgta aataataaca
     6421 atatagacaa taacaatata aataataata attaatatat attatataag caaaaatttt
     6481 agaatcatta gttgtacatt ttatgtttaa tgttttaaat ctagttataa tattcgtgta
     6541 tttaagattg gttttggatt ttagacaagt ttttttatat tatataaatt aaaatattat
     6601 aaaaaataaa ataaaaatag gaacttttac aaatttaccc atcaaataaa tttgtaccta
     6661 attttcaaaa gcaactttat gtactataat atagaaagta gattaaaata gtagacggat
     6721 gtttacagta aatgaaattt taactaatag aaaatcgaag ggagatatac aaatgtcagt
     6781 tattgaatta gtatatgcta gagaagtatt agactcaaga ggaaatccaa ctgtagaagt
     6841 tgaagtagta ttagaagatg gagcaatggg aagagcaata gttccatcag gagcttctac
     6901 aggagctttt gaagctgttg aattaagaga tggagataag ggaagatact taggaaaagg
     6961 tgtagaaaca gctgttgcta acgtaaatga aataatagca cctgaaatag aaggaatgga
     7021 tgcatttgac caaccagcta tagatgcaat catgatagaa ttagatggaa ctccaaacaa
     7081 aggtaaatta ggagctaatg ctatattagg tgtttcaatg gcagtagcta gagcagctgc
     7141 tgatgaaata ggtttaccat tattccaata tttaggtgga gtaaatgcta aacaattacc
     7201 tgttccaatg atgaacatat taaacggtgg agagcatgct gataacaacg ttgatgttca
     7261 agaatttatg atattaccag taggagcttg ttgtttcaaa gaaggtttaa gaatgggagc
     7321 agaagtattc cattcattaa agaaagtttt aggagaaaaa ggattagctt gtggtgtagg
     7381 cgacgaaggt ggattcgctc caaacttagg ttcaaacaga gaagctttag aattaatagt
     7441 tgaagctata acaaaagctg ggtataagcc aggtgaagat gttatgttag gtcttgacgt
     7501 tgctgctaca gaaatgtaca acaaagaaac taaaaaatac gttttagcag gagaaggaaa
     7561 agagttaact gctgctgaaa tggttgcttt atatgaagat tggtcaaaca acttcccaat
     7621 aataactata gaagatggtt tagatgaaga agattgggat ggatggaagt tattaactga
     7681 aaaattagga aataaattac aattagttgg tgacgactta tttgttacta acacagagag
     7741 actagaaaaa ggaattgaaa atggagtagc taactctata ttagttaaag ttaaccaaat
     7801 aggtacaata actgaaactt tagatgctat agaaatggct aagagagcag gatatactgc
     7861 tgttatatct catagatcag gagaaactga agattctact atagcagatt tagctgttgc
     7921 agtaaatgct ggtcaaataa aaactggtgc tccatcaaga actgatagag ttgctaagta
     7981 caaccaatta ttaagaatag aagaaatggt tggagagcaa gctagatact gtggattaaa
     8041 atctttctat aacttaaaaa aatagttatt aagattaaag aaatagtata aaacgtttaa
     8101 gtttgtctta tataaggata agtgttagtt gatttttcaa ttcacttatc cttttttata
     8161 tattattacc taattttatt cacagcttga gaaaaaatat gaataaatgt ttctatctca
     8221 cctttatcta attgatgtag gatattttta aatacagttg cttctatttc tctatgttct
     8281 ttttgagctt ttaaacctaa ttcagttaaa tctaaattat acgccctttt atccttaaag
     8341 caaggatttc tatttataag ttttttagct caagacgttt tacatcactt gttaatgtgc
     8401 tttttggaaa ttctaaagtt gaactaattt cttttatcat actatttgga tgttgctcga
     8461 ctatttctaa aatacttaat tctactaaag aaataccaga aatagtagga tatttaccac
     8521 tacacatttc tgttttcatt tgtataccaa cgttgtgcca taggttgctt agttctttta
     8581 tagcattcat tgacatttac ctccatataa tacgattatc gtactaatat aatatgatag
     8641 tcgtattaat ttgtaaatag gatggttttt tatgaattta gatatttccg tccagcgttt
     8701 tggggaacgc ctgttgcaaa aaaaggatta caattttgta ttgatggatt aagaaattta
     8761 ggatatccat atataacaat atgggttcta aaagataata ttagagttat aaaattctat
     8821 aaaaaaatgg ttttacttgt gatgattttg agaaagatat atgtattgga aaaaaattat
     8881 tagaaatatg ttacctaaaa aagacacagt aattatatat ttttttgtat gtgatttata
     8941 gcataatagt cttgaaatgg ctatattatt gtgatacact aaagagtaat gaaatttaag
     9001 taataaacag ataggagggg caagaatgag caacatttta atgggtgtgc aagtagtgct
     9061 agggatttta ctagtaatta gcataatgcc tcaagattca aaaaatgctt taccttcaga
     9121 gtttggtgga gagggaagtc aagcttactt taaaccaaaa gggaaacaag catttcttgc
     9181 tagagttaca aagataacag cagtattatt ttttataaat gcattggcat tacttatagt
     9241 taagaagtag tgatattgtt gaataactgg ccattattta attagtgcat ttgctagaca
     9301 tttaaaaatt aaatttagtt tttaataaaa gtaagtcaac ataaaaaata cgtaaaataa
     9361 aagctggact aatgatttta tatgattagt ccagttttta ttttatagat tgactatcta
     9421 ttaatatcta ttgtttgtat ttatatattt tttaaatcaa gcaataaata acaacagtac
     9481 tttcaacatg tttcacaaga taatatacat aaagttatat agtaaaaata gtataatcat
     9541 atgtttttat gtaactaaat caattttata tatgtttctc ataatactaa tttatcctat
     9601 tgcatatcat taaataagat tattctgttt taaaataaat attattaaaa atactgtaat
     9661 tatagaaaat gcagaagtaa atactacaat ttgtccagct aattcactat cagaatccat
     9721 ctgttgtgac attgtaaatg aagaaactgc agttggagca gataacatta tcataagtga
     9781 agctaattct acatttctaa atccaagaaa aatactaatt ggcaaaaata tagcaggtac
     9841 aagtataagt tttccacata ccccaaatat aataggtttt atatgtcctt ttatagcaga
     9901 aaaactaaaa gaacctccta gaattataag cgataaagga gtagctatct tagaaatatc
     9961 accaatagtt ttgtgtaaaa atgtaggtat attaatattt aaaatgagca ttgtcaatcc
    10021 tataaaagag gcgattatga gtggatttgt taaaacactt ttaatcatat gttttatatt
    10081 tactttgcct cctctaaaaa tttcaagtat tataacagat aatacattaa acataggtac
    10141 aataactgca atcaataaag atgtgacacc aatactctta tcaccaaata gtgaaattgt
    10201 cacaggaata ccaaatataa cgaaattact acgaaatatg ccttgtatta atgcacccct
    10261 tgtcttatta tttttttcaa ttttaggaat aataaagact aagattaaga agattgaaaa
    10321 aataattata acagctacag ccataagttt aaaattaaga gaaccatcta aatctgtatt
    10381 gtatatatta aaaaataata ataatggcaa gaaagacttg aaacagacat tattcatttt
    10441 atttaaagta tggacatcaa atagacctaa tttttttaga acatatccaa gtgacattgt
    10501 gagaaacaaa ggaagaacaa catttattga taggattaaa ttttccaagg tttactctcc
    10561 aggattctat gattcgataa ttcaattaaa tttacgtcag tttttttaaa acaaatagat
    10621 ttcatattac taaagacctc tgtacactct acaattactc gcttaatttg aatctcattt
    10681 tgttctaagt atttttttgg ctcatcaata tttgtatcta ttttaaagca taaattagta
    10741 ctgattgttt gtacattttc accttgtggt aaaaattcat gaccttttat gtttagattt
    10801 atcttaacat ttccagcatt aaatttatat ttttcacttg tttccttaaa tgaaaaacca
    10861 aattttttat aaaaatttat gtaattttct atattattag tagtaatgag tacatgttta
    10921 attttttttg tcataacaac tccttaagat tttataaaaa ttatttaaat ttatatgggt
    10981 tattaaattt tatatagatt gataatgtct ataaaaatta ttaattttta tgtcgattga
    11041 taaattttat ataagttatt gcatttcatt tagattataa aactttatgt agagttactg
    11101 agtttaacat atattattat ttatattaat gcttttattt taatgagttt aataatattt
    11161 ctaatagaaa taaaaatatg taagcaagtt ttaagctaaa atcaggttat ataaattgat
    11221 aataatctaa tggaaattaa aataccaata aattaaaata taaattaata taatatgtaa
    11281 aaaatattct aataacaatt aagctaatca tcaattaaat gtaggcactt aattaatgat
    11341 tagctatagt caatctaatt aattagaata acttcctttt agtgtaaatg tatctaaaac
    11401 atgtccttca atagctttga aaaattcatt catatagaat cctttttcaa ggtcaaggat
    11461 ggtatcaagt gcaaatacat gtatttcata gacatgaggt ctgtctggag gtgccattcc
    11521 accatataca ctggcatcca atctatcaag acctcctagt ttaccatgcc agctagtagt
    11581 cccttgtaca aaatcagtgg ctgttacact ttcattttcc tttaactcat cttttgttat
    11641 atttacagct aaccagtgta tccatgcaaa cccacacaca ggaacagaat ctttatcttc
    11701 taaaaacaca gcaaaggata ctgtattttt tggagcatct tcaaacttta gaggtagtga
    11761 atatgtaggc ataccacctt cattgaactg agttcctctt ttaccatatt tatcttctat
    11821 aacaccattt actatacctg tactagttac tttcataata atttacctcc caatataata
    11881 aattaataga atatctctat aattcaatta tataccagtt gaaaataatg gtaaattact
    11941 tacttttttg tgggtatcta tttactttaa aacacctttt tccttaagaa tatcttccat
    12001 accattatcc aacataaagt taattcctat atcttgcatt atagccaaag catccaatat
    12061 ttgtttccct cgaataggtg taagtgagta ttcaacttta agtgggtaac ctttatatgt
    12121 ctttttatct acaagaccaa attcaattaa ttctttaagt tgttcaagta acatcttttg
    12181 attaatacca ttaatttcct tttcaagttt tgataaggaa gttggtccta tgcgtaatct
    12241 ccataatata atagatttcc atttgccttt tatcatatca tgtgttagtt ctaatgggca
    12301 agtatactca tcacgaagtt tcaaataagt tcacccccat tttactttgc caagatacgt
    12361 tttgaagcaa gcttatatac aaactcaact tttttatttg atatatccca tgaatctaat
    12421 tcttttttta ttaaatctgg atatttttta cttatatctc taagagcatt acctaaagac
    12481 tttctcaagt attcgctatc atcttctttt aaactagaga gtagtgatat agcgacatca
    12541 ggattagatt taaaatagtc tctactagtc caaattctaa gaccttctgt cacagctctt
    12601 tttatgtttg gactatcaga attaagccat tcttttataa caggaagtgc cttttcatat
    12661 cctacttcac tgcaataata atcaaatgat ttagctaata tttcttgtac tctccagttt
    12721 tcgtctttac tcacattgtc ttttaaaaat aacaaagctt cagaaatagt tactgatata
    12781 aaaccaagta taaacacagc taattctctt acttgataat attctgacct atataagtag
    12841 attgatagat aataacactt ttttgagtca ttatttttca caattttttt agcaatattt
    12901 tcaatttctt taaagccatt ttctatagta ataatttttg caattacttc ttccaatttt
    12961 atctcctcca ataagtagat tagtttaaat tttatgtatt tatttgtaaa aaggtttgtt
    13021 taatttagac aaaataaaga cttgtcaaaa aacgtttgca agtctttagt actagcataa
    13081 ataaaaaata ataaaaaatc aacctcattt aacatataaa tttactttta ttataagttt
    13141 tatattataa agttgtattt aaaaaattaa ttgagttata taaattttac atgatttgag
    13201 tatactaata gaataaatta ttatatagta atacttatat gagatgaata ttttaccaat
    13261 cttatttgat agtgtataat taggtgataa agagtaactt aagcaattat gataaagaat
    13321 aatttaagta attataatga tatttacaaa taatttataa tttacaaatc atcataattt
    13381 gtttgaaatt agtatgataa agcttacatt aaatcaaaaa tataaatatg taattttaac
    13441 ttgtaacaat aataaaaaat atagaaagct atgtgataag taaaacatgt tttgtctgaa
    13501 aaatataaaa gacaatctaa ataataatat aatgaaaagt aaaataggag ggaaaaatga
    13561 taccaggact taaagaaagt ttgctaggat taataaatga aagtgcatat aatcctctaa
    13621 aaaaagaaga attagcagaa atatttgata tacattcatc agaaatgcca atgttttata
    13681 attttttaaa tgaattagaa gaagatggtt atatctgctt tactaaaaaa ggaaaaataa
    13741 tatctccaaa tcaaatggga tactttgttg gaaaatttgt atcgcacaaa aaaggttttg
    13801 gatttgtaga atctgatgtt gaatacacac aggatttatt tatttcaagt gacaatataa
    13861 atggtgctat gcataacgat agagttatgg cagaaatagt agtacctgca actgaagaaa
    13921 agagagctga aggaagaata gtaaaagtaa taaagagaga aataactaga atagtaggta
    13981 ctttccaatc aagtaaaact tttggatttg taacaccaga caacaagaaa tttactaagg
    14041 acatatatat ccctaaaaaa cattttagtg gagcagtaga taatgataaa gtagtatgtg
    14101 aaattacagt atggccacaa gaggatagaa aaccagaagg taaaattata gagatactag
    14161 gtcaaaaagg tgaaagaggc gtagaaatag attctataat aagagaacat ggtcttccag
    14221 aagaatttcc aaagaaagta ttgcaagaag cagaagctgt agctgttgag attcctcaag
    14281 aagagataaa aagaagaaga gatttaagag atttaaatat atttactata gatggagatg
    14341 acgcaaaaga cttagatgat gctatatcaa tagaagtatt atctaatgga aacttcaaac
    14401 taggagttca catagctgac gttacacact atgtaagaga gaaaaataaa cttgacaaag
    14461 aagctttaaa aagagctaca tcagtttatt tagtagataa agtaatccca atgttgccaa
    14521 agactctttc aaatggagta tgtagtttaa atccatttga agataagttg accctttcta
    14581 tatttatgga aatagaccat aaaggaaatg tggttaaaca tgaaatatgt gaatctgtaa
    14641 taaattcaaa agctagaatg acctatacag aagtatctga tatattagag aaagatgatg
    14701 aaaaacttaa aaagactttt gaacatgttg ttgaagattt taaaaatgct gaagttttag
    14761 ctaggattct tatgtcaaga agggaaaaga gaggggctat agactttaat ttccctgagg
    14821 caaagataat tttaaatgga aaaggtgaag ttgtagatat aaaaccatat gaaagaagaa
    14881 tatctaataa gataatagaa gaatttatgc ttataagtaa tgagacaatt gctgaacatt
    14941 tttattggat gggaatacca tttgtgtata gaatacatga gacaccatct ttggaaaaaa
    15001 tggaagaatt gagtaagttt atatctacat ttggatatat aataaaaggt gataaagagg
    15061 aagtacatcc aaaagcatta caaggtataa tagagaaaat aaagggcaaa aaagaagaag
    15121 gagctatcag tactataatg cttcgttctt taaaacaagc taagtactca ccagaatgta
    15181 gtgggcactt tggattagca gctaaattct actgtcactt tacttcacca ataagaagat
    15241 atcctgacct tcaaattcat agaataataa aagaatcttt aaataataaa ataagcggaa
    15301 aaagacaaga acagcttact actatagtgg attatgcatc tgttcaatcg tctgagaaag
    15361 aaagaaaggc agagttagct gaaagggatg tacatgattt ctataaggct ctttatatga
    15421 aggataaagt tggtcaagaa tttgagggtg ttgtatctag tgtaacttca tttggaatat
    15481 ttgttgagtt agataatact atagaaggct tgataagact tgcaaatatg aatgatgatt
    15541 actatgttta tgatgagagt tcttatagtg ttttaggaga aagaactaag aagtcatata
    15601 agataggtga ccttgttaag ataaaggttg agagtgtgaa tgtagatttt aaggaaattg
    15661 actttgagat agttgaaaag atagaggagt agataaaaca gctaaaataa gagataacaa
    15721 attgtggtgt ttgtgatatg gataaatata tagtgcacaa tactcgataa aataaattat
    15781 aaaaacaggt aattaagaaa tgtttacatg gaagtagcca actgaaagat aagaaattaa
    15841 gaggtattta atagaaatag ataaatgaga aataagaaac taagaagcat ttagtagaaa
    15901 tagctagctg aaaaataaga aattaagaag catttaatag aaatagctaa ctgagaaata
    15961 agaaattaag aaatatttaa tagaaatggc taactgaaaa ataagaaatt aagaagcatt
    16021 tagtagaaat agctagctga aaaataagaa attaagaagc atttaataga aatgtataac
    16081 tgaaaaataa gcaactaaga aacatttagt agaaatagct agctgaaaaa taagaaatta
    16141 agaagcattt aatagaaatg tataactgaa aaataagcaa ctaagaaaca tttaatagaa
    16201 atagataaat gaaaagcaag taattaagaa ctatttaatg gaaacagata atttaaaatt
    16261 acctaattaa gaatagatat aaaaaaatat attttcgata aaaaataagt gtatcattta
    16321 aaatttaaga tacacttatt ttttatttaa gagaactttt ccttttatat aatttataac
    16381 aagaggttta atatatttat aattaatttc aaaattattc atatctgcca aattaaattc
    16441 ttacatatat aattattggt atgcttatta acacttcaga aatcattata gtttaaattt
    16501 tgagtttatt gttattactt atttaatttg agcacattat ttatttttat ttgcaactaa
    16561 taaatagagt atgattttat aatacagttt cttaaatttt ttttaaaagt aatagttctt
    16621 atttgtagcc aatattgctt atagaactta ttgattcatt taatttttag aaaaaataat
    16681 aatacttaca caataaacac agaattttat atagataata taggtattta aatttaaggt
    16741 gaaatgatac ttttcttaat atattttatt gtaaataagc ttgattataa gttaaaatta
    16801 aaaaaaacac tacttaggag gaatgtaatt atggggatga taggatgcta tacaaaaata
    16861 agtgaggaga atgtttttaa attacagcaa gcagaagaaa atttacaaga tttcgttttt
    16921 gaagattcca acgaaaatag tactattaat attgataaag catggcatgc cattcacttt
    16981 actttgacag gttgtccttt tggtggagat gaagataata tatttagcaa actagtgctg
    17041 agtggaaata tacttatgga aattgatgga gaatttcctg tcatgttaat tacagctaat
    17101 gatgtaaaaa aactatcaaa agcaatgaat agtttggaag aacaagagtt tagaaaaaga
    17161 tttaatatta atgagatgtt agaaaacaat atttatcctg ttatgaatga tgagagtgaa
    17221 gatgattttt ttgaatatgt atgggcaaat ctaattgagc ttaaaaggtt tatacaagaa
    17281 gcatctgatg atggacaagc tgtaatattt tttataatgt gataatcact aaaaaattgt
    17341 gaatattagt gtcatcacat atcttagata tgaagaccat agaaaaatat ataaaagaaa
    17401 tgattggtga aagtattagc gaaataaatt cactttggaa aactggtgaa agtataaaaa
    17461 taaattattc taaagtatct tctgaaagat ttgaagaaat aattcaaaga gtaacacctt
    17521 aggaggagtg tctacatatt ttttgacaag ttacaaatat ttggagcagt ttcagctacc
    17581 tataatgaat ctatgatgat atagtatcct caaaatcatt atctcaagtt attgtacaat
    17641 taattaggat actaatgtca attataagta tagcggtatt aatcataggt tttgtaaata
    17701 ttttaatata tcttggaggc gattttttag tagttgagta tatttggaaa tttcgcattg
    17761 gctatgtcta taggaagttt aggaatgttt ttctcaaatt tatttaataa tacaatcata
    17821 ggttatatgg tatctatggg atatttaatt ttaaatatga tgactaaagg caagtatgtt
    17881 ggaaattttt ttataatgtc tatgagtcaa agtagttttg atgagaaata ttagttgata
    17941 cttggaagta ttgtgttgat aattgaatct atcttaataa aaccaataaa aaggaagatt
    18001 agataataaa atgaggctta gataaaataa aggtaataca aacaaaaagt acatttatga
    18061 aatatgaatt tatttttaca tacacaaacc tacttataat ctctttatgc atgtagatag
    18121 atttatatat cagtagtata aaatacatat gtataccgaa ttttatcaaa agttcccaaa
    18181 tttatatttc aaagacccca aagacactta ttgctatgac ctacgtacat gtattttttc
    18241 attttaattt ttatattagc aatatgacta gtgtcgtaaa ctattgatac ctcataacaa
    18301 aaaattctcc atcccctaaa tatgttttct gtctcttagt ctcaactatt ttaaatcctt
    18361 gagactcata acattttaca gctctcatgt tccaggttct tacatttaat acaattggtt
    18421 tagtactata tttatttcta cattcttgta atcctaggct aattatttct ttaccaattc
    18481 ccattccgca atactctggt tttataccaa taccaaagaa tacagagctt ccttcatcta
    18541 ataagtttat aaaacctatc aattctttat tgtcgtttat aaaacttata aaattttttc
    18601 ttctatcttc ttttgctagt gaaaaattct tctttttaat ttcctcccat ggaggtagat
    18661 tgtatattga atatttacct tcatatttcc aattaataac ttcaatagca tgttcttcag
    18721 taaatttaac tttatttaaa ttcatatgtc ctcctattaa aatatttaag gtaattaatg
    18781 gtattttaaa attttagaaa ataagtaaaa ctatttatat ttatccatga aatctttaaa
    18841 aattttacta acaattcttg taccctgtat tttttattgg attcacagaa gactataaac
    18901 taattttgaa ttaccttcta attcaaaaac ctcacaatat atgtaacttc ttagcttgat
    18961 taaattctgt taaatctgtt tcttatagat ttcttatagt tgtataatca acatcaatat
    19021 atttatgtgt attagttaat ttattgactt taggaactta ttattaatat gttatatttg
    19081 cttcaatact ccaatagtag ggtaaattgt aggcaaatta tcaaatgtat atctatccat
    19141 gcttaaactt tagagataca ataactacta aattagtatt ctagtagtta taaaaaagaa
    19201 tctttaaaaa tattaattta acttatattt tttaataaat tttctaaaat ttaatcagtt
    19261 aatttaatta tagctctaaa tgatattttc tacagataga atttcaaaaa gatgtgtata
    19321 agaacaagtt cacttaaatt tttaaaatca aataaagttg aatctcttga ttaccttgat
    19381 tattttatga acttaatata gatgtaaact ttactattaa aaaaatacga gattttagat
    19441 aagttaatgc taatttaaat aataagataa gaaatataat atgacataag agctaggaat
    19501 aagataaata taatcaaaga ataaggcatg agaataatta ccaagagaaa tcgttacttt
    19561 aatatgttta tgaatgttag actatgttcc taaattgaat tttatatatc aaatataata
    19621 acttacagaa tgtggaaatt tttatttaaa gaatacacat aaacatttcc acactctgat
    19681 tttataataa taatctaata aaatttatag tgactttata aactcaataa tgatttaata
    19741 aatatttata aaacaactat ttttgcttag aaactttatt attcatatta tcattagtag
    19801 gcattgttcc aaaacttagt ttttcataca ttacttgagt tggagctagc caaactgtac
    19861 cattaccagt aaatgtattt aagaaacctt caccatttaa agcagaacca attaaagatt
    19921 tatctgactt tgtaacagaa aaattaacac cagatgttct tgctattgca aagttaccat
    19981 ctacctttaa ttcttcacca tttttcaatt catactctac aatttcactt tgaggaacaa
    20041 cacattccag aacgattaca ccagtaccac ttaattctat ttggaagaag ccttcaccac
    20101 caagtaaagc agatgagaag ttcttttgtg taaatgcttt tacttctatt gtatcagagc
    20161 aacaatagaa taatccttta tcaacaatta tggaatcatt gtcaagactc ataaataaat
    20221 agtgcctaaa agaaggttcc aaataaactt ctccagtacc cttgtataca ggtttcatca
    20281 ttgattcatt agtaagtgct cctgatattc cttttttgaa aagacctcca acaccaccaa
    20341 tcttagtttc agaagatata tttcctttat aatagtataa tgcaccagct tctgttttta
    20401 ttgatgaatt atttaatttt atcttaattt gtctagcttt caaaccagct tctctagcaa
    20461 aatataatcc cattgccatc attgggctag aagctccctc aagtttttca aattctaata
    20521 tctcaaattc gcttttgcca tctgatgcat tttctaaaat ttttactttt ttgcttaaat
    20581 ccatacaaat ttctcccgac tatgttaaga tttatctcta aaatattata agatataatt
    20641 atatattaca ttttttttga aaaaatggct actttatcat caaattttta aatattttta
    20701 ataagttttt gaaatgttta gatttaagtt aaattgttta agaacatata cttgtttcaa
    20761 taggtatttt gagtattatt tattgctttt gggtattata ttttatgggt atgttaaaat
    20821 tat
//
LOCUS       DAFNNB010000061        20374 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0061, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000061 DAFNNB010000000
VERSION     DAFNNB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0061"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..2491)
                     /locus_tag="L3M60_001884"
     CDS             complement(<1..2491)
                     /locus_tag="L3M60_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6226551.1"
                     /translation="MNSNTSIKNKDSNPKIFLVLTLYLLGIFMGAIDTGIVSPARTII
                     QNSLGVNEKTGIWMITIYTLTYASVIPISGKLADKIGRKYVYLVSIFLFGSGSLICGL
                     SSLFSNFYILLIGRVIQAIGGGGIMPIATAEFGTTFPENKRGMALGLVGATYGIANIL
                     GSSIGSTILSIFGTQNWKWLFFVNLPICLIILIGGVFCIKNNKSESKEKIDKLGTLML
                     VCIIVSLLYGLMNIDFFNFKNSIQDISVYPYLLTFIVLIPIFIFIENRAKDPILSFEY
                     FLNPRILIILILSLIVGIGMMGMVFVPQYAENALKINAGSGGYFVAILGLFAGVAAPL
                     SGRLIDKFGAKKILLLGFSVSMIGSLYLVLIALKTNTVFSVCLSLMFMGLGMGFTMGT
                     PLNYMMLSNTRLEESNSALATLSLIRSIGTSISPAIMIGFIAHAGLSVQDNIMDLVGK
                     PSTPKIVQLEELENMIDDLKSDPEMAKNLKNVSIPNMNDTSNINMDMTGGKLPKYLLD
                     KVQSADTTNITNITKEISTTMFDEKVPSIISKIQKNVQKGIDGTQSGINGIEEGESKL
                     NSGIKGVQTGIDNMVKARAGINQGIDGVKKGIAGIDKGIKGMEQGLKKQDKAIDELTT
                     AYNKIPTTEKIPTNNENNSNEQNQDNASNSESDATNNDSTENKNNNSNDNKPNLNEQK
                     ESLNAQIQKLKKTRNELNAKLQKNKAQKNELSKKLKSMESQKKELQSKLNNSINQKKD
                     MEKSLDTMQQQKKSLQAVLEKTQEVKTEIPKAFDKSKLDYINSIENNRTKIENTLQST
                     LNSGFKQMYITVFCVNLLAFVILLFYKENKTR"
     gene            complement(2505..3047)
                     /locus_tag="L3M60_001885"
     CDS             complement(2505..3047)
                     /locus_tag="L3M60_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6226552.1"
                     /translation="MPKILENVKEDILKVSRDMILEEDYSNISIRKIAQRCGISAGTV
                     YNYFNSKQEIIEYITKSEWDLLIRRIEYSNKNTEDYIKKLDIIFTEIRNFINKVHNVQ
                     YNDFLNTFETERFFEMKKHKDDFHEQLSNKVYEALEKESFFNNDKLVCNIIIKMFFSY
                     STYLHIEFEDLKPYIEKLIH"
     gene            3291..4805
                     /gene="glpK"
                     /locus_tag="L3M60_001886"
     CDS             3291..4805
                     /gene="glpK"
                     /locus_tag="L3M60_001886"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBN6226553.1"
                     /translation="MEKKYIMALDQGTTSSRAILFNKKGEIVKIAQKEFNQIYPKAGW
                     VEHDPMEIWGSQSGVMREVIETAGIRPEEIASIGITNQRETTVVWSRYTGKPIYNAIV
                     WQCRRTSEICDELKNKGLEESIKEKTGLLIDAYFSATKVKWILDNVEGAREKAEQGEL
                     LFGTIDTWLIWNLTRGKVHVTDYSNASRTMMYNINDLEWDKDILRELDIPISMLPLVK
                     PSSYVYGHTDERMLSGAKIPIAGCAGDQQAALFGQNCVEEGTAKNTYGTGCFLLMNTG
                     SNIVKSKHGLLTTIAWGVDGKVTYALEGSIFIGGASIQWLRDELKIIESAKDSEMYAN
                     RVEDTNGVYVVPAFTGLGAPYWDMYARGSILGLTRGAKKEHIVRATLESIAYQTKDVL
                     EAMQNDSKLKLKSLKVDGGASNNNFLMQFQSDILNVNIDRPKIVETTALGAAYLAGLS
                     VGFYIERNEITSKWQVEKEFNPNMSEEKRCKLYKGWKKAVSRALSWEKEDELDI"
     gene            complement(4916..5545)
                     /locus_tag="L3M60_001887"
     CDS             complement(4916..5545)
                     /locus_tag="L3M60_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226554.1"
                     /translation="MDKNYTMPIYQKIALDIANKIYTGEIQEDSVLFGRSVLAGKYNV
                     SPETIRRAVKILEDIGVVKSIKGKGVIVLSPDKASSFIKKYRDITNISSYKSTLYNLI
                     DTKSNLENEILDTINKILDYSNRLEIINPLVPVQFTINSNCKYIGQTAAKTKFWQNTG
                     ATIVAIKRGEELIISPGPYIEFLEGDILLVVGDQHIYNSIPMFLYENEK"
     gene            6132..6923
                     /locus_tag="L3M60_001888"
     CDS             6132..6923
                     /locus_tag="L3M60_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HBN6226555.1"
                     /translation="MYKVDLNSDLGESFGTYKIGLDEEVLKYISSANIACGFHAGDPS
                     HMEKTVQLAKKNGVKIGAHPGFLDLIGFGRREMKITKQEAKDYTKYQLGALMAFASSN
                     GCNIQHVKPHGALYNMAAKDKELAMGICEAIYEVNKDIILLGLYNSEMINSAKEIGLR
                     FANEVFADRAYDNNGFLVPRNAEGAVIHDTKHAIDRVVRMVKEGTVETLTGEVIHIKA
                     DSICVHGDNPKAIEFVKEIRKRFELESIEVCPLENIEVCSSENIV"
     gene            6952..8172
                     /locus_tag="L3M60_001889"
     CDS             6952..8172
                     /locus_tag="L3M60_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HBN6226556.1"
                     /translation="MSTKNITDKTKNKKDVGALIGAAFIMATSAIGPGFLTQTAQFTQ
                     DFGPNFSFVILITTILFIGAQVNVWRVIGVSGLRGQDIANKIIPGLGYLVAFLVALGG
                     LAFNIGNVGGAALGMNVMFNMNMTLGTVLSGLIAIFVFMSKNSNSLVDKITKFLALGM
                     IIIVGYVAISNHPPVGEAVSRMVKPENPKGLIFPIITLLGGSVGGYITFAGGHRLIDG
                     GITGEENIKEITKSSLLGILVATMMRVLLFLAILAVVSKGLQLDPENPAASAFKFSAG
                     AIGYKFFGLVLWSAAITSVIGAAYTSVSFLKTLNPFIDKYEKYFIIAFIAISTLIMAF
                     IGKPATLLILAGALNGLILPITLGIMLIASKRKDIVGDYKHPTWLLIFGLIVVLISAY
                     TGITSLSSLGALFA"
     gene            8192..8887
                     /gene="pxpB"
                     /locus_tag="L3M60_001890"
     CDS             8192..8887
                     /gene="pxpB"
                     /locus_tag="L3M60_001890"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBN6226557.1"
                     /translation="METRYLISGDKAVVAEFGNEISEDINKKVISFMRAIEISNLKGI
                     VTEMVPTYRSLMISYNPLKIDFDSLIESLKKIEDNLESIVLPKPKIHEIPVCYDEVFG
                     IDIKNVASYNNLTVDEVIKIHTSREYLIYMLGFTPGFPYLGGMDERIATPRLEIPRTK
                     IYGGSVGIAGSQTGVYPIDSPGGWQIIGRTPLKLYDENREEQILLRAGDFIKFVPITL
                     DEFIEIEKNNLSI"
     gene            8917..9903
                     /locus_tag="L3M60_001891"
     CDS             8917..9903
                     /locus_tag="L3M60_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase"
                     /protein_id="HBN6226558.1"
                     /translation="MGFKVELGGFQTLIQDRGRVGYGQYGVSGCGAMDEYAHRVGNIL
                     VGNSEDEASLEVLMLGPTITFDEYTQIAVTGGDLGAKINGKEIQNWRSYQINPGDVLS
                     FRGVKSGARAYVSISGGIDVPLAMGSKSTYTRAKIGGFEGRALKKGDYISTFIQEKDF
                     TINKKLNSKYIPTYSSEIVLRIVKGPQFDAFSKGEVEKFLSNEYKVTNEIDRMGCRLD
                     GESIKHINGADIISDGISYGAIQVPGHGKPIIMLSDRQTSGGYTKIGNVISVDLYKLA
                     QAKPNDVVKFELVDIYEAHRLLREQEDKIQDIYKSMKNIRVVKAKVLNDIAV"
     gene            complement(10121..10687)
                     /locus_tag="L3M60_001892"
     CDS             complement(10121..10687)
                     /locus_tag="L3M60_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226559.1"
                     /translation="MLGEKMRNIRKSKKKTLSDVSKLTDLSISYISQIERDAIEPSLS
                     SLRKIAEVLDTPLYMFMDDNNTDDLVIRKEDRVMMKFPKSEMFYEIVSPMPTPEFTPS
                     ILFVEFELKPESEDAKDYIYHASEEIMVVTSGVVDVCMGEKIVRLNPGDSTFIKANVP
                     HKIINPSKDTIARGYGVISPPIWPIKSK"
     gene            11038..12105
                     /locus_tag="L3M60_001893"
     CDS             11038..12105
                     /locus_tag="L3M60_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HBN6226560.1"
                     /translation="MKITDIKFEKLRIKLKKPVVVSFGVIEYGESIILKIETDEGYCG
                     FGEAAPLAAVTGEVLDNVISVLLMFKKELIGKDPLDIETIHTIMDGIIIGNTSAKAAI
                     DIALYDIKGKIMNVPLYKVLGGFDSKVQTDITISIDKPEKMAIEALERVKEGFRILKL
                     KAGINPEDDIEAVKLIREAVGESIRIRIDANQGWNVNSSINTIKKLEEFNVDAIEQAL
                     PHWDLDGTAYIRNKSNIKIMIDESLHSPIDAIKAIKKNAVDTFNIKLMKSSGIYPAIK
                     INNIAEASGVNCMLGCMLETRIGITAAANLIASKKNITEADLDSFMFCEESKSISGGF
                     VMDGDIMNLLNKPGLGIEVNL"
     gene            12124..13284
                     /locus_tag="L3M60_001894"
     CDS             12124..13284
                     /locus_tag="L3M60_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBN6226561.1"
                     /translation="MNTLFSNPGSILAVMTLMIALGFYLQRYKAIKSLGPALTIIIMG
                     IILSNLKVVPVSTELYGTISTYAIPVSMTIMLMSVDLKEMTKLSREPLIAIFVAVLTV
                     SIMAFLFGLVFAEKISEGWKVAGMFVGTYTGGSANLTAIGTGLNVSRQTLAAANAADY
                     VIGVPTLIFMFALPAILKNSKKFKKLWPYHVEESELEDCQNEEFMESKEWSIKDIAWM
                     LAIGFVVTEVSTILAGYFNSSFSSAARILLVTTISIIIAQLKPVKKLKGNLDLGLFVA
                     LFFLCTIGFSVDIKEFLGSTFTITLYCFSIIFTSFVFHLGITRLLKIKYQYVILSIVG
                     AIADGPTSALVAASAKWNSLVSVAVVMGVIGGVLGNYAGISVAYAIKMSLGL"
     gene            13297..15393
                     /locus_tag="L3M60_001895"
     CDS             13297..15393
                     /locus_tag="L3M60_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HBN6226562.1"
                     /translation="MIKFKLYISGLYSGNVIFDDDLLIEKLNPFTNKIESLKPISREE
                     NTYYLNLTKIDLKSLFNNFDVYTKSLVNTDKDTVINNLGETHSKLNEYIWVQRNKKFP
                     LDIIIVDNKIVGFICLSRETCTILIMDGYEEYTVLKEWDKTHKDEEIYSIRFGGNYMI
                     DMKDGIKLSTDVYLPNFIDSTKKAPTILMRTPYGKENDKEIYYKYVQRGYAVVIQDVR
                     GRNESEGKWKPMIHEREDGDSTINWIVSQEWSDGTVGMLGASYLGYVQWAAASSGNKH
                     LKALVSIVTSGSPFIDIPRKGGAFVSGMLAWAFMVSRNKVDRSKMVRDDWDDVLNIRP
                     IENIPVEALGYRIEFLEEWLKRVEKDEYWDLMDWHLQKDKINVPALVVSGWYDDNGMG
                     TTEALDVIKDYEKGKRKAILGPWMHNSNTLRDINGISLGNSSLRYDLDYNYLLWFDKY
                     LKGIENNIDTTAPIEYYSVGSNIWKTEENWPIINKIDKSMYLISDGNANTSLGNGRLV
                     FDSRLEEKCDSYIYNPKDPSIQLIDMSENEVGVPNNYKDLEKRSDMLCYTSDVFSEEF
                     TITGDIKLEFFASSSARDTDWVIKIMDVDLDGNSIKLADGILSARFRNGFYESEFMEE
                     GKIYKFTIITSKISNTFKVGHKIRLDITSSAKNFIFQNSNTTEGYNSIEYIEAKNTIY
                     HGGKYPSKLILPIENK"
     gene            complement(15554..15898)
                     /locus_tag="L3M60_001896"
     CDS             complement(15554..15898)
                     /locus_tag="L3M60_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBN6226563.1"
                     /translation="MEIKRYEGTGRMSRAVVHNNTVYLCGQTHAEGGVIEQTTEILAK
                     IEDLLNKYGSDKQHLLSVTIYLRDMKDFEAMNSVWDAWVEKGFEPARACVEARLAREH
                     LLVEMSVVAATK"
     gene            16190..17080
                     /locus_tag="L3M60_001897"
     CDS             16190..17080
                     /locus_tag="L3M60_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBN6226564.1"
                     /translation="MSSTIKSNRIKGIMFIIASALGFAMMSAFVKLAGDLPSFQKVFF
                     RNLVSAIIAFWLIIKHKGSLTGKKENLKVLLYRSIFGTLGVIFNYYAIDKLMLSDANM
                     LNKMSPFLVVIFCAIALKEKINVKQIGAIIIAFIGALFIIKPTFSVEVIPYLGGIAGA
                     VFAAMAYTCLRVLGDREDYYTIVFFFSVFSLVTVGPIAFAVYEPMTLMQLIYLLLGGV
                     FASLGQFGITLAYKYAPAKEISIFDYSNIIFSAILSIFLFNVYPDMLSVVGYLIVFSA
                     AFYMFLYNKKLDKLDKEKKK"
     gene            17298..18584
                     /locus_tag="L3M60_001898"
     CDS             17298..18584
                     /locus_tag="L3M60_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBN6226565.1"
                     /translation="MEKVKVAVIGAGNRGTYAYAPYIYENSDVCEIVAVAEPKKGRRE
                     LFTQKYNLDSKNVFETLEDFFKKDKMADAVIIATNDDRHYDVAKLALEKGYHVLLEKP
                     MSNSLDGLVHINDLCDKYKDRLFMICHVLRYSPFYNKLKEIVESKKLGELVSIQYNEN
                     IGYWHFAHSFTRGNWRNSNETSPLILAKSCHDMDILLYLVGSRCKKISAFGSLKHLNN
                     QNASGEMAQNCLQCLVEKKCPYSAKRIYLEKDRSINRAVHINPTKENLLDILKTSPYG
                     RCVYRCDNNVVDNMVNILQFENGVTATFNLCAFTKENGRTIKLMFSHGEVGGDLNKNE
                     IRIKAFGKNEEIVINPSNQQNMVEEYDRNLIAEFIKLVSNKELEKGRVAAKEAIQSHV
                     MAFAAEYSRVSDEVVYIEEFFDNAKQMTKEIEETIF"
     gene            complement(18673..19116)
                     /locus_tag="L3M60_001899"
     CDS             complement(18673..19116)
                     /locus_tag="L3M60_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory YrvL family protein"
                     /protein_id="HBN6226566.1"
                     /translation="MIKISNKRRIPLGEFISVFLFFAVFFSITFCIFTVIGIGFLSFL
                     GFEYKSLGAVLIFFLIYFCITTPIDFLCTTILDIFRYVNKLPYSIYKLCEFIIDFILT
                     FLAMNIIDTFMDSVTIPLSTEILFALLSYLLSECMDFFDKDKKKP"
     gene            complement(19140..19934)
                     /locus_tag="L3M60_001900"
     CDS             complement(19140..19934)
                     /locus_tag="L3M60_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HBN6226567.1"
                     /translation="MKLYLSADIEGTCGIVNWDETKLECEFSQHYRTQMSKEIAAACD
                     GATQSGVHEILVKDAHDSGRNINPNILPENVKILRGWTKDPLVMMAGIDESFDACIFT
                     GYHSGATCNGNPLSHTMDIDYDYFKINDEIASEFTINAYIAAYYNVPVAFLSGDEMLC
                     ESAKKLNPNIVTVPVSKGIGNGSISIHPNLALKRIEKGVKKALSGDLSRHLIKLPDKF
                     KIEIKFREHYKAFKASFYPNMKKIDSQTVLFETEDYYEFLRMLLFI"
BASE COUNT         7602 a         2444 c         3391 g         6937 t
ORIGIN      
        1 atctagtttt attttcttta tagaacaata atattacgaa tgctagtaga tttacacaga
       61 atactgttat atacatctgt ttaaatcctg agtttagagt agattgtagt gtattctcta
      121 tttttgttct attattttct atggaattta tataatccaa ttttgattta tcaaaagcct
      181 ttggtatttc agtttttacc tcctgtgttt tttctaaaac tgcttgtagt gatttttttt
      241 gctgttgcat tgtatcaagg cttttttcca tgtctttctt ttggtttata gaattattta
      301 acttactttg tagttctttc ttctgagatt ccattgattt tagtttttta cttaattcat
      361 tcttttgagc tttatttttt tgtaactttg catttaattc atttcttgtc ttttttaatt
      421 tctgtatttg agcatttaaa ctttcttttt gttcatttaa atttggttta ttgtcattgg
      481 aattgttatt tttattttct gtggaatcat tattagttgc gtctgattca gaattactgg
      541 cattatcttg gttttgttca tttgagttat tttcattatt agtaggaatt ttttctgtag
      601 taggtatttt attgtaagca gttgtaagtt catcaatagc tttatcttgc tttttaagac
      661 cttgttccat cccttttata cctttgtcaa taccagcaat accttttttt acaccatcta
      721 ttccttgatt gataccagct ctagctttta ccatgttgtc gataccagtt tgaactcctt
      781 taataccact atttagttta ctttcacctt cttcaatacc gtttattcca ctttgtgttc
      841 catctattcc tttttgtaca tttttctgta ttttagaaat tatagatgga actttttcat
      901 caaacattgt tgttgatatt tcttttgtta tatttgtaat gttggttgta tctgctgatt
      961 gaactttatc aagtaagtat tttggaagtt tgccacctgt catgtccata tttatatttg
     1021 aagtatcatt catatttgga atagagacat tttttaaatt ttttgccatt tcagggtctg
     1081 attttaagtc atctatcatg ttttcaagtt cttcaagttg aactatttta ggcgttgagg
     1141 gttttccaac taagtccata atattatctt gtacagataa tcctgcatgt gctataaatc
     1201 caatcattat agcgggagaa attgaagtac caattgagcg tatcaaggat aatgttgcaa
     1261 gtgctgaatt agattcttct aatcttgtat ttgataacat catgtagttt agaggtgttc
     1321 ccatagtaaa tcccatacct aatcccataa acattagact tagacataca ctgaatactg
     1381 tattggtttt tagagcaatt aagactagat aaagagaacc tatcatagac acagaaaatc
     1441 ctagtaatag tattttcttt gctccaaact tatcaataag tcttccgctt aaaggagcag
     1501 caacacctgc aaataatcct agtatagcta caaaatatcc accagaacca gcatttatct
     1561 ttaatgcatt ttcagcatat tgtggaacga ataccatacc catcattcca ataccaacaa
     1621 ttaaagataa aataagtatt atcaatattc ttggatttaa aaaatattca aagcttaaaa
     1681 tagggtcttt agctctattt tcaataaata tgaaaatagg tataagtaca ataaatgtca
     1741 ataagtatgg ataaactgat atatcttgta tagaattttt aaaattaaaa aagtctatat
     1801 tcataagacc atataatagt gatacgatta tacataccaa cattaatgta ccaagcttat
     1861 ctattttttc tttagattca gatttattgt ttttaataca gaaaactcca ccaataagta
     1921 tgattaaaca gattgggaga tttacaaaaa atagccactt ccaattttga gttccaaata
     1981 tgcttagaat tgtgctacca atacttgaac ctaaaatatt ggcaatacca tatgttgctc
     2041 caactaaacc taaagccata cctcttttat tttctgggaa ggtagttcca aattctgctg
     2101 tagcaatcgg cataattcca ccaccaccaa ttgcttgaat aacacgaccg attagtaaaa
     2161 tatagaagtt ggaaaatagt gatgataatc cacatattaa tgaaccacta ccaaatagaa
     2221 atatactaac tagatacaca tattttcttc ctattttatc tgctaatttt ccagagatag
     2281 gaattacaga agcatatgtt aatgtgtata tggtaatcat ccaaattcct gttttttcat
     2341 ttactccaag gctattttgt ataattgttc ttgctggtga tactattcca gtatcaatag
     2401 ctcccataaa tatacctaat agataaagtg taagtactaa aaatattttt ggattactgt
     2461 ccttgttctt aatactagta ttagaattca aggtaaacac caccttaatg tattaatttt
     2521 tcaatatagg gttttaaatc ttcaaattct atatgaagat atgttgaata tgaaaaaaac
     2581 atttttatta tgatattgca tactaattta tcgttattaa aaaagctttc tttttctaaa
     2641 gcttcatata ctttatttga aagctgttca tgaaagtcat ccttgtgttt tttcatttca
     2701 aaaaatctct ctgtttcaaa agtatttaaa aagtcattgt attgtacatt atgaactttg
     2761 tttataaaat ttcttatttc agtaaaaata atgtctagtt ttttgatgta atcttcagta
     2821 tttttatttg agtattcaat tcgccttatt aataaatccc attcagattt tgtaatgtat
     2881 tctattattt cttgttttga gttaaagtaa ttatacactg tacctgcgct tataccacac
     2941 ctctgagcta ttttccttat gctaatattc gagtaatctt cttctaaaat catatctcga
     3001 gatactttta atatatcttc ttttacattt tctaaaatct ttggcatatt accacctctt
     3061 tcattaaaaa tattatgaac actgtttatt atactccttt aaaatttatt aatcaatatg
     3121 taagctataa tgtataatat tattatgtag atatgataga aaataagtgt cttcttataa
     3181 attatctatt gtattaaatt tgtacttaag tatgattatt tattgaaata tacaatgtta
     3241 aattttcttt ttaataaatt aaatatttat atgataagga gaatatgatg atggaaaaga
     3301 agtatataat ggcattagac caaggaacaa ccagttcaag agctatacta tttaataaaa
     3361 aaggcgaaat agtaaaaatt gcacaaaaag agttcaatca aatttatcca aaagctggat
     3421 gggtagagca tgaccctatg gaaatatggg gttcacaaag tggagtaatg agagaagtta
     3481 tagaaacagc aggtataaga ccagaagaaa ttgcttcaat aggaataact aaccaaagag
     3541 agactacagt tgtttggagt aggtatactg ggaaaccaat ttataatgca atagtatggc
     3601 agtgtaggag aacatcagaa atatgtgatg aacttaaaaa taaaggattg gaagaatcaa
     3661 taaaagaaaa aacaggtctt ttgatagatg catacttttc agcaacaaaa gtaaaatgga
     3721 tactagataa tgtagaagga gctagggaaa aagctgaaca aggagagtta ttatttggaa
     3781 ctatagatac atggttaata tggaacttaa ctcgtgggaa agtacatgta acagattact
     3841 caaatgcatc tagaacaatg atgtacaata taaatgattt agaatgggat aaggatatat
     3901 tacgagaatt ggatatacca ataagcatgt taccattagt aaaaccatct agttatgttt
     3961 atggtcatac agatgagcgc atgttatcag gagctaaaat acctattgct ggatgtgctg
     4021 gagaccaaca agcagcattg tttggtcaaa attgtgttga agaaggtact gcaaaaaata
     4081 cttatggaac aggttgtttc ttacttatga atacaggcag taacatagtt aaatctaagc
     4141 atgggttact tacaacaatt gcttgggggg ttgatggaaa agtaacatac gcattagaag
     4201 gaagtatatt tattggagga gcatcaattc aatggcttag agatgaatta aagataatcg
     4261 aatctgcaaa agatagtgaa atgtatgcaa atagagtaga ggatactaat ggtgtatatg
     4321 tagttccagc atttacaggc cttggagctc cttattggga tatgtatgct agaggttcaa
     4381 ttttaggtct tacaagagga gcaaaaaaag aacatatagt aagagctaca ttggagtcaa
     4441 ttgcttatca gacaaaggat gttttagaag caatgcaaaa tgattcaaaa ttaaaattaa
     4501 agtcattaaa agttgatggt ggagcaagta ataataattt cttaatgcaa tttcagtcag
     4561 atatattaaa tgtaaatata gacagaccaa aaatagtaga aactacagct ttaggagctg
     4621 cttatttagc aggattgtct gttggatttt atattgaaag aaatgaaatt acttcaaagt
     4681 ggcaagtaga aaaagagttt aatccaaata tgagtgaaga gaagagatgt aaattatata
     4741 aaggctggaa aaaagctgta tcaagagctt taagctggga aaaagaagat gaattggata
     4801 tttaaaaatt aatcaaggtt aaatttattt gaatttaaaa agagctttat gtaaaatatg
     4861 aagctctttt tttgttaatt ttgtattttt atataatcat actaaattat ataatttact
     4921 tttcattttc atataaaaac attggtatag agttgtaaat atgttggtca ccaacaacta
     4981 agagaatatc tccttctaga aattcaatat acggtccagg agatattata agttcttcac
     5041 ctcttttaat tgcaactatt gtagcacctg tattttgcca aaatttagtt tttgcagctg
     5101 tttgaccaat atatttgcaa ttagaattta tggtgaactg aacgggcact aaaggattga
     5161 ttatttcaag tctgttagag tagtctaaaa ttttatttat agtatctaat atttcatttt
     5221 ctaaattaga tttagtatct attaaattat aaagtgttga tttatatgat gatatatttg
     5281 taatatctct atattttttt ataaaagaag aagctttgtc tggagatagt acaataacac
     5341 ctttaccctt tatgctttta acaacgccaa tgtcctctaa aattttgaca gctcttctta
     5401 tcgtttctgg agatacattg tatttaccag ccaaaacaga gcgtccaaat aatactgaat
     5461 cctcttgtat ttcacccgtg tatattttat ttgcaatatc caacgctatt ttttggtata
     5521 ttggcatcgt atagttttta tccattgttt ttcccccaat acaaaattta tgttaaaaaa
     5581 ttatataatc ttaacaatac tattgtataa tttataggtt tatatgtcta caatatgtac
     5641 aaatattgtg catgttttaa aataatattt taacatgcat ttagtataca tgattttatt
     5701 atacttaaaa ctatataaag aattaattat aaaaatataa agatatttta tttacaaaga
     5761 atgataatta tatagatgaa acaaacaaat gaatgaagaa aaatgattct gtcttgcata
     5821 taaagtgatt aaaaatgaaa aatactttaa aaatgaaggt taaagccttt gttttaagtg
     5881 aaaataatta aatgttaact aaaaaatggt aaaaacattt tttgaaaaag aaatacaaat
     5941 gaatgtttga ttaaaataat tgcaaaaata ttccttaaat ggtaaaatta tattaattaa
     6001 atgcaataga atagttgcaa aaataaaatt gaatacaagt aataaaatat ttcatgcaat
     6061 atataaaata aacaaaattt aaaaattgaa actacttatt ctatcaaaat gttagcaagg
     6121 ggggtcataa attgtataaa gtagatttaa atagtgattt aggagaaagt tttggaacgt
     6181 ataagattgg tttagatgaa gaagttttaa aatatatttc atcagccaat atagcttgtg
     6241 ggtttcatgc aggtgaccct agtcatatgg aaaaaacagt acagctagct aaaaaaaatg
     6301 gagtgaaaat aggagcacat ccaggttttc tagatttaat aggatttgga agacgtgaga
     6361 tgaaaataac gaagcaggaa gctaaagatt atacaaaata tcaattaggt gctttaatgg
     6421 catttgcaag ttcaaatgga tgtaatatac aacatgtgaa accacatgga gcattataca
     6481 atatggcagc taaagataaa gagttggcta tggggatttg tgaagctata tatgaagtca
     6541 ataaagatat tatattgttg gggctatata atagtgaaat gataaattct gctaaagaaa
     6601 ttggacttag gtttgcaaat gaagtatttg cagatagagc ttatgataat aatggttttt
     6661 tagttccaag aaatgcagaa ggagcagtta tacatgatac aaaacatgca atagataggg
     6721 ttgttaggat ggtaaaagaa ggaactgtag aaactttaac aggtgaagtt atacatatca
     6781 aagcagattc tatatgtgta catggggaca atccaaaagc aatagagttt gttaaagaaa
     6841 taagaaagcg ttttgaatta gaaagtatag aagtttgtcc attagaaaac atagaagttt
     6901 gttcatcaga gaacatagta taagactgat aacatgaggg ggagataaaa tatgagtaca
     6961 aaaaatatta cagataaaac taaaaataaa aaagatgttg gagcgcttat aggggctgca
     7021 tttataatgg caacatcagc aattgggcca ggatttttaa ctcagactgc acaatttact
     7081 caagattttg gaccgaattt ttcatttgta attttaatta caacaatcct ttttataggg
     7141 gctcaagtaa atgtatggag agtaattggt gtatcaggac ttagaggtca agatatagca
     7201 aataaaataa tacctggatt aggctattta gtagcttttt tagtagctct tggaggatta
     7261 gcttttaata taggaaatgt tggtggagct gcacttggta tgaatgtaat gtttaatatg
     7321 aatatgacat taggaactgt attaagtgga ttaatagcta tatttgtatt tatgtcaaaa
     7381 aattcaaatt ctctagtaga taagataact aaatttttag cattaggaat gataattata
     7441 gttggatatg tagcaatatc aaatcatcct cctgttggag aggcagtttc tagaatggta
     7501 aaaccagaaa atccaaaagg attgatattc ccaataataa ctctacttgg aggaagtgtt
     7561 ggaggttata taacttttgc tggaggacat agattaatag atggtggaat aacaggagaa
     7621 gaaaatataa aagaaataac taaatcttct cttttgggta tattagttgc cacaatgatg
     7681 agagttcttt tatttttagc aatactagca gtagtttcaa aaggtcttca acttgaccca
     7741 gaaaatccag ctgcttcagc ttttaaattt agtgcaggag caataggata taaattcttt
     7801 gggcttgtat tatggtctgc tgcaattaca tcagtcatag gagctgccta tacatcagtt
     7861 tctttcttaa agacactaaa tccttttata gataaatatg aaaaatattt cataatagca
     7921 tttatagcaa tatcaacact tataatggca tttataggaa aaccagctac tttattaatt
     7981 ttagcagggg ctttaaatgg attgatactt ccaatcacat taggaattat gcttatagca
     8041 tctaagagaa aagatattgt tggagattac aagcatccta catggctctt aatatttgga
     8101 ctaatagtag ttttaatatc tgcatataca ggaataactt ctcttagcag cctaggtgct
     8161 ttatttgctt aatttagaag aggtgacatt tatggaaact agatacttga tatcagggga
     8221 taaggcagtg gttgctgaat ttggaaatga gatatcagaa gatataaaca aaaaagtaat
     8281 ttcatttatg agagcaatag aaatatcaaa tcttaaaggt attgtaacag aaatggtgcc
     8341 aacatatagg tcacttatga taagttataa tccactaaaa atagattttg atagtttgat
     8401 tgagagttta aaaaaaatag aagataattt ggaaagtata gtattaccaa aacctaaaat
     8461 acatgagatt ccagtatgct atgatgaggt ttttggtata gatattaaaa atgttgcaag
     8521 ttacaataat ctcactgtag atgaagttat aaaaatacac acaagtagag aatacttgat
     8581 atatatgcta gggtttactc ctggatttcc atatctaggt ggtatggatg aaagaatagc
     8641 tacacctaga ttagaaatac caagaaccaa aatttatggt gggagtgttg gaatagcagg
     8701 ttctcagact ggtgtatatc ctatcgatag tcctggtgga tggcaaataa ttggtagaac
     8761 acctttaaaa ttatatgatg aaaatagaga agaacagatt ttgcttagag ctggagactt
     8821 tataaaattt gttccaataa cattagatga atttatagaa atcgaaaaaa ataacttaag
     8881 tatttaaata tcgttatagt acagggagtg gtatatatgg gattcaaagt tgaattagga
     8941 ggtttccaaa cattaataca agacagaggt agagttggat atggtcaata tggtgtatct
     9001 ggatgtggag caatggatga gtatgcacat agagtaggaa atatattagt aggaaattct
     9061 gaagatgagg cttctttaga agttttaatg ttaggcccta caattacctt tgatgaatat
     9121 acgcaaatag cagtaactgg gggagattta ggggctaaaa taaacggaaa agaaattcaa
     9181 aactggaggt catatcaaat aaatccaggt gatgtattat catttagagg tgtgaaatca
     9241 ggtgctagag cctacgtatc aatctctggt ggaatagatg ttccacttgc aatggggagt
     9301 aagtcaactt atacaagagc taaaattggt gggtttgaag gtagagcact taaaaaagga
     9361 gattatataa gtacttttat tcaggaaaaa gattttacaa taaataaaaa attaaattca
     9421 aaatatattc caacttatag ctcagaaatc gtactaagaa ttgtaaaagg tcctcaattt
     9481 gatgcttttt ctaaaggtga agtagaaaaa tttttatcaa atgaatacaa agttacgaat
     9541 gaaatagata gaatgggatg tagactagat ggagagagta taaagcacat taatggagca
     9601 gatattatat ctgatggtat ctcttatgga gctatacaag tacctggtca tggaaaacct
     9661 attataatgt tatcagatag acagactagt ggtggttata ctaagatagg aaacgttata
     9721 agtgtagatt tatataagtt agcacaagca aagccgaatg atgtagtaaa atttgaactt
     9781 gtggatatat atgaagcaca tagactattg agagaacaag aagataagat tcaagatatc
     9841 tacaagagca tgaagaatat aagagttgta aaagcaaaag tattaaatga tatagcagtt
     9901 taatttaatt ttaataaata taatttttat tataatttaa aatataactt acaaatagct
     9961 atttactatt taccataatt aagatggttt agattagtca aatagctatt tttttagttt
    10021 gattaaatat gttaaattta aataaatact tcaattttaa aataaaagtg atatttaaat
    10081 ctattgtagt atctataaaa agtagatatg tttattgaat ttatttagat tttattggcc
    10141 aaattggagg tgatataact ccatatccac gagctattgt atccttactt gggttgatga
    10201 ttttatgtgg tacattagct tttataaatg ttgagtcacc tggatttaat ctcactatct
    10261 tttcgcccat acaaacatcc accacaccac ttgttaccac catgatttct tctgatgcat
    10321 gatagatata atctttggca tcctcgcttt ctggtttaag ttcaaactct acaaataaaa
    10381 ttgaaggtgt aaactcaggt gtaggcattg gagatactat ctcgtaaaac atttcactct
    10441 ttggaaattt catcataact ctatcttctt ttctaatgac taaatcatca gtattgttat
    10501 catccataaa catatataaa ggagtgtcta aaacttcagc gatttttctc aatgaagata
    10561 aggatggttc aatagcatct ctttctattt gagagatata acttatagat aaatctgtca
    10621 atttggaaac atcacttaat gtttttttct tactttttct tatatttctc atcttttcac
    10681 ctagcataag aacctccaaa taataaaatt aatataaaat attcgcttaa aacaaagtaa
    10741 ttatagcata acatagaagt agtggcaata agaatactta gtagattttg acaattaaaa
    10801 tttaattaaa ggcaaagaaa agtctatgaa aataaattat tgctaaatta acataaagag
    10861 aataataata aaaaaattta atggaagagt gttaattgaa ttaatattat tacattatat
    10921 tttggaaaag cgtaaatttt cagtaaatta tcttgacata tatattgaag taataatata
    10981 atttaaagta taaaatttta aaaattgaat tataacttca aaaataagga gggtaatatg
    11041 aagataacag atatcaaatt tgaaaagctg agaattaaat taaaaaaacc tgtagtagtt
    11101 tcatttggag taattgaata tggagaaagt ataattttaa aaattgaaac tgatgaaggc
    11161 tattgtggat ttggagaagc agcacctctt gcagcagtta caggtgaagt tttggacaat
    11221 gttatttcgg tattacttat gtttaaaaaa gagttaatag gtaaagaccc actagatatt
    11281 gaaaccatac atacaatcat ggatggaatt attataggca atacttctgc aaaagcagct
    11341 attgatatag ctttatatga tattaaagga aaaattatga atgtacctct ttataaagtt
    11401 cttggaggtt ttgatagtaa agtccaaaca gatattacta taagtataga taagccagaa
    11461 aaaatggcaa tagaagcttt agaaagagta aaagaaggtt ttaggatatt aaagcttaaa
    11521 gcaggtataa atcctgaaga tgatattgaa gctgtaaagt taattagaga agctgttgga
    11581 gagagtataa gaattagaat tgatgcaaat caaggatgga acgtaaatag cagtataaat
    11641 actatcaaaa aattggaaga atttaatgtt gatgcaattg aacaagcact tccacactgg
    11701 gatttagatg gtactgctta cataagaaat aagagtaata taaagattat gatagatgaa
    11761 tcattgcatt caccaataga tgcaattaaa gctataaaaa agaatgcagt tgatacattt
    11821 aatattaagt tgatgaagtc aagtggaata tatcctgcta taaaaatcaa caacatagct
    11881 gaggcaagtg gagtaaattg tatgttagga tgtatgctag aaacgagaat tggtataaca
    11941 gcagcagcaa atttaatagc atctaaaaag aatataacag aagctgacct tgatagcttt
    12001 atgttttgtg aagaatcaaa aagtatatct ggaggatttg taatggatgg agatattatg
    12061 aatcttctca ataaaccagg tttaggaata gaagtaaact tatgataaaa ttggaggtta
    12121 aatatgaata cacttttttc aaatccaggt agtatacttg ctgtaatgac attaatgatt
    12181 gcattgggtt tttacttaca aagatacaaa gccattaaaa gcttaggacc agcacttaca
    12241 ataatcataa tgggaataat actttcaaac ttaaaagtag ttccagtaag tactgaatta
    12301 tatggaacta tttcaacata tgcgattcca gtttctatga ctataatgtt gatgagtgtt
    12361 gatttaaaag aaatgacaaa gttatcaaga gaacctttga ttgcaatttt tgtagcagta
    12421 ttaactgtaa gtatcatggc atttttattt gggctagtat ttgcagaaaa aatatcagaa
    12481 ggttggaaag ttgctggaat gtttgtaggt acatacactg gtggaagtgc taatcttaca
    12541 gccattggaa caggtcttaa tgtaagtaga caaactcttg ctgcggcaaa tgcagcagac
    12601 tatgtaattg gagtaccaac attgatattt atgtttgctc taccagccat attaaaaaac
    12661 tcaaagaagt ttaaaaaact ttggccatat catgtagaag aatcagaatt agaagattgt
    12721 caaaatgaag aatttatgga gtcaaaagag tggagtatta aagatatcgc ttggatgttg
    12781 gctataggtt ttgttgttac agaggtctca acaatacttg ctggatattt caattcaagc
    12841 tttagtagtg cagcaagaat acttttagtt acaacaattt caattataat tgctcaatta
    12901 aagccagtaa agaaactaaa gggaaattta gatttagggt tatttgttgc attattcttt
    12961 ttatgtacaa taggtttttc agtagatata aaagagtttt taggttcaac ttttactata
    13021 acactttact gttttagcat aatttttact tcttttgtgt tccatttagg cataactaga
    13081 cttttaaaga taaaatatca atatgtaata ctttctatag taggagcaat tgctgatggt
    13141 cctacttcag ctttagtagc tgcttctgca aaatggaatt cacttgtaag tgtagcagtt
    13201 gtaatgggag taattggtgg agtattagga aattatgcag gtatatcagt ggcatatgca
    13261 ataaagatgt ctctgggatt atagggggga atttaattga taaaatttaa attatacata
    13321 tctggactat acagtggaaa tgtaatcttt gatgacgatt tattaattga aaagttgaat
    13381 ccttttacaa ataaaataga aagtttaaaa ccaataagca gagaagaaaa tacatactat
    13441 ttaaatctta caaaaataga tttaaagtct ttatttaata actttgatgt atatactaaa
    13501 agtttagtaa acacagataa agacacagtt ataaataact taggagaaac gcattctaaa
    13561 cttaatgaat atatatgggt tcaaagaaat aaaaagttcc cactagatat aattattgta
    13621 gataataaaa tagtaggttt tatttgttta tccagagaaa cttgtactat attaattatg
    13681 gatggatatg aagagtatac tgtattaaaa gaatgggata aaactcataa agatgaagag
    13741 atatattcta taagatttgg tggaaattat atgattgata tgaaagatgg tataaaactt
    13801 tctacagatg tatatctccc taattttata gattctacca aaaaagcacc aacaattctt
    13861 atgagaactc catatggaaa agaaaatgat aaagaaatat attataaata tgtacaaaga
    13921 ggatatgctg ttgtaataca ggatgttaga ggaagaaacg aatcagaagg taagtggaaa
    13981 cctatgattc atgaaagaga agatggagat agtactataa actggatagt atcacaggaa
    14041 tggtcagatg gaacagttgg aatgcttgga gcatcatatc ttggctatgt tcagtgggca
    14101 gctgcatctt caggtaataa gcatcttaaa gctttagtta gtatagtaac atcaggaagc
    14161 ccttttattg acataccaag aaaaggagga gctttcgtat ctggtatgct tgcatgggcg
    14221 tttatggtat caagaaataa agttgataga agtaaaatgg taagagatga ttgggatgat
    14281 gttttaaaca tacgaccaat agagaatata cctgttgaag ctcttgggta tagaattgaa
    14341 tttctagaag aatggttaaa gagagtagaa aaagatgagt attgggattt gatggattgg
    14401 catttacaga aagataaaat aaatgtacct gcgttggttg tatctgggtg gtatgatgat
    14461 aatggtatgg gaactacaga ggccttagat gttataaaag actatgaaaa aggaaagaga
    14521 aaggctattc taggtccatg gatgcataat tcaaatactt taagagatat aaatggtatt
    14581 tctttaggaa atagttcatt aagatatgat ttagattata attatctgtt atggtttgat
    14641 aaatacttaa aaggtataga aaataacata gatacaactg ctccaataga gtattactct
    14701 gtaggttcta atatatggaa gacagaagaa aattggccaa taataaacaa gatagataag
    14761 agtatgtatt taataagtga tggaaatgct aacacatctt taggaaatgg tagattagta
    14821 tttgatagta ggttagaaga aaagtgtgat agttatatat ataatccaaa agacccatca
    14881 atacaactaa ttgatatgtc agagaatgaa gttggagttc caaataacta caaagattta
    14941 gaaaaaagaa gtgacatgtt gtgttataca tcagatgttt ttagtgaaga atttactata
    15001 acaggtgata taaagttaga atttttcgca tcaagttctg ccagagatac tgactgggtt
    15061 ataaaaatta tggacgtaga tttagatgga aattcaatta aattagctga tggtatactt
    15121 agtgctagat ttagaaatgg tttttatgag tcagagttta tggaagaagg aaaaatttat
    15181 aaatttacga ttataacttc aaaaatatca aacacgttta aagttggtca taaaattaga
    15241 ctagacataa catctagtgc taaaaacttt attttccaaa atagtaacac gacagaggga
    15301 tataatagta ttgaatatat agaagcaaag aatacgattt atcatggagg taaatatcca
    15361 tctaaattga tattgccaat agagaataag taaatttata tagattaaat agtgaggagg
    15421 tgtctcaaaa tgaacttttt ggttcgtgag gcactttctt ttatatgaaa tcgaatatat
    15481 tttcatcatt tcaacaataa aaaagaggct tatctctcta ttttgagaca gcccccccta
    15541 taagctaaat tgattatttt gtagctgcaa caacgctcat ttcaactaac aaatgctctc
    15601 tagcaagtct agcttcaaca caagctctag caggttcaaa tcctttttca acccaagcat
    15661 cccaaacaga gttcatagct tcaaagtctt tcatatctct taaataaata gtaacagata
    15721 gtaaatgttg cttatctgaa ccatatttgt ttaataaatc ttctatttta gccaaaattt
    15781 cagttgtttg ttcaattact cctccttcag catgagtttg tccacataaa taaactgtat
    15841 tgttatgaac tacagctcta ctcattcttc cagttccttc atatctcttt atttccatat
    15901 tttaaatacc tccatagtta aaaaatctat taaaaatcta aataataaat taaaaaaagt
    15961 ctatactata taaaattata ttatatattc tgataataat ccataaaaac atataacttt
    16021 ttaactataa ttattattgt tattaattga aaagatttat tagttacttg acatagttta
    16081 cacttttaat tatagtataa attggaatat ttagataatt attatttaat gtacaagcta
    16141 agtttttaat taatacggct ttatacattt atatagaaaa gaggtacgta tgagtagcac
    16201 aatcaaatca aatcgaataa aaggtataat gtttataata gcatcagctc ttggctttgc
    16261 aatgatgtct gcatttgtaa aactagctgg agatttacca agttttcaaa aagtgttctt
    16321 tagaaatctg gtatcagcta taatagcttt ttggcttatc ataaaacata aaggtagtct
    16381 aactgggaaa aaagaaaatt taaaagtact tttatatagg tcaatatttg gaactctagg
    16441 agttatattt aactactatg caattgacaa gttgatgctt tcagacgcta atatgctaaa
    16501 taaaatgagt cctttccttg tagttatatt ttgtgcaatt gctttaaaag aaaaaataaa
    16561 tgtaaagcag ataggagcta ttataattgc atttatagga gctttattca taataaaacc
    16621 aacatttagt gtagaagtaa ttccatatct tggaggaatt gcaggagctg tatttgcagc
    16681 aatggcatat acttgtttaa gagttcttgg agatagagaa gattactata caatcgtatt
    16741 tttcttttcg gttttttcat tagttactgt agggccaatt gcttttgcag tatatgaacc
    16801 aatgactctt atgcaactta tatatctatt actaggtgga gtatttgcaa gcctaggtca
    16861 atttggaata actctagcat ataaatatgc acctgctaag gaaatatcta tattcgacta
    16921 cagtaatata atcttttcag caattttaag tatattctta ttcaatgttt atccagatat
    16981 gttaagtgtt gttggatatt taatagtatt ttcagcagca ttttatatgt ttttatataa
    17041 taagaaatta gataaattag acaaagaaaa gaagaagtaa gctagataaa agtgttttta
    17101 taatttgtat tgaatattta agtctttgta ttaaaattaa agaaaattta attaaaaatg
    17161 tttggtatat taacttataa cagcagttaa aattgaaaaa ttataactgc tgtttctttt
    17221 attaaaaata tccaaatatc cgtaaaaata gactataata gagtagtata aataatataa
    17281 aggggataaa ttaaagtatg gaaaaagtga aagttgctgt gattggagct ggtaatagag
    17341 gaacgtatgc atatgctcca tatatttatg aaaattctga tgtatgtgaa atagttgctg
    17401 tagcagagcc taaaaaagga agacgagaac tatttacaca aaaatataac ctagatagta
    17461 aaaatgtatt tgaaacttta gaagactttt ttaaaaagga caagatggca gatgcagtta
    17521 taatagctac aaatgatgat agacattatg atgtagcaaa actagctctt gaaaaaggtt
    17581 atcatgtatt attagaaaag ccaatgtcta atagtttaga tggtttggta catataaatg
    17641 acctatgtga taagtataaa gataggcttt ttatgatttg tcatgtattg aggtattctc
    17701 cattttataa caaattaaaa gagattgtag aaagtaaaaa attaggagaa ctagttagta
    17761 tacaatataa tgaaaatata ggatattggc attttgcaca tagctttact agggggaatt
    17821 ggagaaatag caatgaaaca agtcctttaa ttttagccaa gagttgtcat gatatggata
    17881 ttttattata tttagtagga agtagatgta aaaaaatatc tgcatttggg agcttaaagc
    17941 atcttaataa ccaaaatgct agtggagaga tggcacaaaa ttgtcttcag tgtttagtag
    18001 aaaaaaaatg tccatattca gcaaagagaa tttatttaga aaaagataga agtataaata
    18061 gagctgtcca tataaatcca actaaagaga atttattgga tatacttaag acaagtccat
    18121 atggaagatg tgtttataga tgcgacaata atgttgtaga taatatggta aatatattac
    18181 agtttgaaaa tggagtgacg gctactttta atttatgtgc atttactaaa gaaaatggaa
    18241 gaactataaa gttaatgttt agccatggtg aagttggtgg agatttaaat aaaaatgaga
    18301 taagaattaa agcatttgga aaaaatgaag aaatagttat aaatccatca aatcaacaga
    18361 atatggtaga ggaatacgat agaaatctta tagctgaatt tattaaatta gtttcaaata
    18421 aagaactaga aaagggtaga gtagcagcaa aagaagctat acaaagtcat gtaatggcat
    18481 ttgcagcaga atactctagg gttagtgatg aggtagtgta tatagaagaa ttttttgata
    18541 atgctaagca aatgactaaa gagatagaag aaactatatt ttaattaata aaaatttatt
    18601 aaataaaata aagatagatt aagtaataga aatacgaaaa tatgtattct attaagaaat
    18661 ctatcttttt tattaaggtt ttttcttatc tttatcaaaa aaatccatac attcggacaa
    18721 aagataagaa agtaaagcga ataagatttc tgtacttaaa ggaatcgtta ctgaatccat
    18781 aaaggtatca attatgttca tagctagaaa agttagtata aaatcaatta taaattcgca
    18841 aagtttatat attgaatatg gtaatttgtt tacatatctg aatatatcta aaatagtagt
    18901 gcatagaaaa tcaattggag tggttataca aaaatatatt aaaaagaaaa ttaatacagc
    18961 tccaagagac ttatattcaa atcctaagaa tgataagaag ccaattccta taaccgtgaa
    19021 aatacaaaaa gttatactaa aaaaaacagc aaaaaataaa aatacagaaa taaattcgcc
    19081 taaaggaatc cttcttttat tacttatctt aatcaacccc ttaatttatg acatgaaact
    19141 tatataaata ataacattct taaaaattca taatagtcct ctgtttcaaa tagtactgtc
    19201 tgagaatcta tttttttcat atttggataa aaagaggcct taaaagcttt ataatgttct
    19261 ctaaatttta tttctatctt aaatttatct ggcaatttta ttaagtgtct agataaatct
    19321 ccagataaag cttttttgac acctttttcg attcttttta gtgcaaggtt tggatggatt
    19381 gaaattgaac catttccaat tccttttgac acaggaacag tgactatatt tggatttagt
    19441 ttttttgcac tctcacaaag catttcatct ccacttaaaa atgctacagg aacattataa
    19501 tatgcagcaa tataagcatt tatagtaaat tcagatgcta tttcatcatt tattttaaaa
    19561 taatcataat caatatccat agtatgagaa agtggattac cattacaagt agctccagaa
    19621 tgatagccag tgaatataca tgcatcaaag ctttcatcaa tcccagccat cataactagt
    19681 gggtctttgg tccatcccct aagtattttg acgttttcag gtaaaatatt tggattaata
    19741 tttctaccac tatcatgtgc atctttaaca agtatctcat gcacaccaga ttgagtagct
    19801 ccatcacatg ctgctgctat ctctttactc atttgtgttc tatagtgttg tgaaaattca
    19861 cattcaagtt ttgtctcatc ccaatttaca ataccacaag taccttctat atcagcactt
    19921 aaatataatt tcataatata taccccctta tgtttattat taatgataat aacagataac
    19981 agaaaataca taatcaataa agggcataaa aacattatgt cttactatta attattatta
    20041 aatctgtaac tgaaaaataa ctaaactggt attaaaaaat ataacactat gtgtcattta
    20101 tttatatagt tttttatatc tgatgtttat ataaaattgt aacatataag tggttacatt
    20161 tactagttta acatagttaa aatagttagg tattattttt aatttaaatt gattattata
    20221 aaatcgtgga gtaaaagaaa aatttaattt attaaaaatg cacgaaatgt tttatcctta
    20281 acacaatgct aaattaaact caaaacgaaa taatagaaaa atcttatgtt cttagaaaga
    20341 atgtgaatga tttaagggaa aaatagaaat ttat
//
LOCUS       DAFNNB010000062        19457 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0063, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000062 DAFNNB010000000
VERSION     DAFNNB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19457
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0063"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     misc_binding    92..303
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            466..1755
                     /locus_tag="L3M60_001901"
     CDS             466..1755
                     /locus_tag="L3M60_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBN6226568.1"
                     /translation="MEEEKINYALEQIPENQKRGWVAMFSVLVAIGVDLSSVILGAEL
                     AQSMPMKQAILSVIVGSFFSAILYTTCSLVGSSTSLSTSMITKYVFGEAGAKIFSLVI
                     GVSLLGWFGVQVGFFAQNAQIIIKDIFNLDVSMQILSLIGGLLMMSTAIYGYKAMEKL
                     SVYSVPFLLVLMMLTIFLAFRANGIPVDDNMKSTMTFAGGVSLSMSIIIVGAIVSPDI
                     SRWAKSRRDCALSSFLGIQFGNAFMIIVSIVLVKCMGTSDIMRIFITLGIAIPGIIVL
                     TLAQWTTNTSNVYSASLSIALVLKKAPEKVLTIVLGIVATLLAVFGIYEGFIGFLNLL
                     GIVIAPVGGVYTAEYYIVKQELKGFDKGVLYKPIVKRSIVSWIIGILITYLSTYGFIT
                     LTTIAPLDGFIAGFVVQSIIGKVLCSMKNNQEIDKAV"
     gene            1809..2888
                     /locus_tag="L3M60_001902"
     CDS             1809..2888
                     /locus_tag="L3M60_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HBN6226569.1"
                     /translation="MRFLTEESVDKIAVGAAVLGTGGGGDPYVGKLVAKQAIKKYGPV
                     KVISLDELDDDALVVPVSGMGSPVITIEKLLSEVELTTPLEIMEKLLNRKVDVIIPIE
                     IGGINSLMPIAVAAKKGLPILDADSMGRAFPEAQMVTFYLEGYEASPAVMSDEKGNSA
                     ILYPVDGIWSERLARTLTVEMGGSSSISDYNLSGAQVKKAAIGNTLTIAETIGGFLLE
                     SKSDSKGAVENILKELSGYKLFEGKVIDIKRELKGGFTRGHAFFAGINEYDGEYSILF
                     QNENLIAKKGDTPLCITPDLIAVLDLETGFPITTERIKYGSRVMVVAFPCNEKWRTEK
                     GIETVGPGYFGYDVEYKTVEELQGK"
     gene            2909..4453
                     /locus_tag="L3M60_001903"
     CDS             2909..4453
                     /locus_tag="L3M60_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HBN6226570.1"
                     /translation="MYRVGIDVGGTNTDACILDGELKVIHSVKVATTKDVETGVYNAL
                     KKVIDESKIDHSLIKYAMLGTTHCTNAIVERKRLNNVAMIRIGKPATTAILPFIDWPE
                     DLREKIELDSILVSGGYEFDGRKINELCKDEVVEFCNRIKDKVESVAISGVFAPVNKQ
                     QEEEVAKWVREILGDIPISLSNEIGNIGILERENGAILNSALSQVGKAVSLGFEKALK
                     DLGIDAQIYFSQNDGTLMNLEYTMKYPIFTIGCGITNSIRGASFLSKVKNAIVLDVGG
                     TTSDLGVLYNGFPRESSIPVTIGGVHTNFRMPDVLTIGLAGGTCVKGEKDNIKIGPES
                     VGYRITEDAIIFGGNTLTLSDIVTKLDMAFEDRKSNVENLDKEYCDEVYKKVILKLED
                     AVDQMKTSQDDVELVLTGGGSIIVPEKLKGISNVIRPPHYTAANAIGAALGQISGDVE
                     KVYSLDKMSREDAIEDAKKEAVNKALKAGAKEDTIEVLNIEDVPLAYLPGNALLIKVK
                     VVGDLI"
     gene            4568..4759
                     /locus_tag="L3M60_001904"
                     /pseudo
     CDS             4568..4759
                     /locus_tag="L3M60_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5012..5554)
                     /locus_tag="L3M60_001905"
     CDS             complement(5012..5554)
                     /locus_tag="L3M60_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HBN6226571.1"
                     /translation="MKRYIALLRGINISGKNKIVMSELKAGFVELGYSAVSTYLNSGN
                     VVFLSNVDDSEVISNSIGLMIKKQFELEIPVFIISQEVLEDILNNAPSWWGDNNKEIY
                     DNLIFMFPDLSYDKFYDEVGNPKEEYEKVYHYKNAIFWSFSRKDYQKTNWWSKTASSN
                     VSDKITIRTANTVRKIVGMR"
     gene            complement(6003..6287)
                     /locus_tag="L3M60_001906"
     CDS             complement(6003..6287)
                     /locus_tag="L3M60_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6226572.1"
                     /translation="MAKNTKQTTVHIDETLLKEVKRIGINENKSISQIINESIIDYVL
                     VYRENEDKEKNEHDKNLNETINKFKREEEKRIQAKSAARAIAEAKKRVLY"
     gene            complement(6348..7112)
                     /locus_tag="L3M60_001907"
     CDS             complement(6348..7112)
                     /locus_tag="L3M60_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBN6226573.1"
                     /translation="MNNYLKFATYQLIGFTSSIPRLIKIKKNPDKFSLKEKFEFMQKQ
                     AKKSLDIVNIELNIIGKETLPKEPLLFVVNHSSMLDSFILTASVERPIGCVIADEPVW
                     RNIPIFKEWAKLLRCVYVNRKNNREGIKSIAEASQNILTGQSMAVFPEGDLTWIKEPN
                     SLVSEFRSGALKIAYKAKCPIVPLVIKNSKDTYEGYQPIGKINSVPVEVEFLEPIYDH
                     IENPRLKSSVLGENIKNKMIDTIENFRKSNKTFKEF"
     gene            complement(7394..7660)
                     /locus_tag="L3M60_001908"
     CDS             complement(7394..7660)
                     /locus_tag="L3M60_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBN6226574.1"
                     /translation="MIESRCGILCYECEYKEKVNCKGCTDIKKPFWGEQCPVKSCCEN
                     KNLTHCGLCDTFPCEMLNQFAYDKEQGDDGKRIVQCKKWAVLAL"
     gene            complement(7702..8511)
                     /locus_tag="L3M60_001909"
     CDS             complement(7702..8511)
                     /locus_tag="L3M60_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226575.1"
                     /translation="MNKIINNILNTCKQYTYILPHKLLRQYIAHYTISIPDTSIKENL
                     TLVPDASGCMIFKFDKKGIESSFWGATTKTTIVKNDIENVLFRVFVEFRPGGVYYLTG
                     LSQRESTDLKISLEDFNTLFSLEVNSIFERTSTIKELVEQLDMLFLSYLLKANIVDMT
                     IPILENARKQNSIMSVKSISQISCYSERHLNRIFNNSLGMSLKSYLRLLRINLVLQEI
                     QNNKIPFVTLAQDIGYYDQSHFINDFKSICGVNPTTYIKNLSDFYNEKYKF"
     gene            8824..9105
                     /locus_tag="L3M60_001910"
     CDS             8824..9105
                     /locus_tag="L3M60_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226576.1"
                     /translation="MASKQTPRNNLEKFLRIRVLSHTGDKIHIKLPVNFAKRMIENNA
                     LDLFNGKDDVVDGKKVTEILVKAFDYDLTGEVVHLERKNGDIIKVSIVQ"
     gene            9370..9624
                     /locus_tag="L3M60_001911"
     CDS             9370..9624
                     /locus_tag="L3M60_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226577.1"
                     /translation="MENNDFKLTICIFAFVTGVVVLFCCIGLGEFSVEHIIQQNGGTM
                     DTDQYNIYLDQSISQYRNFGSILALLGGLGILFDKYIRKY"
     gene            9669..9992
                     /locus_tag="L3M60_001912"
     CDS             9669..9992
                     /locus_tag="L3M60_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226578.1"
                     /translation="MATEIFTVKITPIEAQKIIKENLNADLVFSDSYNLGNDKFILIT
                     TFEKYYARNNSDAGLIVVYENTTGKTVIKAISTGSAIGVLKIDWGAGGNLLKRVKNIL
                     ADYII"
     gene            complement(10262..10456)
                     /locus_tag="L3M60_001913"
     CDS             complement(10262..10456)
                     /locus_tag="L3M60_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6226579.1"
                     /translation="MINYMIEIIYSEKISEVENILKIENIQYEFTSRDGFIFFGFYDM
                     TDAYKFETLCSDEKIQFKRI"
     gene            complement(10828..12447)
                     /locus_tag="L3M60_001914"
     CDS             complement(10828..12447)
                     /locus_tag="L3M60_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBN6226580.1"
                     /translation="MNIVISLIGGLGLFLYGMSLMGEGLQKSAGDKLKKIIELLTSNV
                     VMGVLVGTVVTGIIQSSSATTVMVVGFVNAGIMNLSQAIGVIMGANIGTTVTAQLVSF
                     NLEGIAPIALGIGILFYLFTSNQKTKHLSEILIGFGILFTGMEFMKDAVAPLAEYKAF
                     TDALLYFSKNPILGILAGFAITGIVQSSSASMGMLIALASQGILPLSSALPILYGDNI
                     GTCVTSLLSSVGASRNARRAAVMHLSFNVIGTIIFMLILNKPISAIVTHFDPTDTARQ
                     IANAHTLFNLTNVIILLPFSKYIVKLANRLIPIKETESEIVNNTKYLDERMFSTPSIA
                     LGNTVQEVVRMGHKATNSLEHSIAGFLNKSNEDINKAFESEKVVNKLQKDILNYLLKL
                     SKEPLRDDERFRTDLLFNTVNDIERVSDHAENIAELAMSVKEMNISFSDSAIREIYEI
                     YNKTITNFKDALIVLDVKDFELANKVLEVENEVNYLEKTFKNSHMIRLNNGSCTIDAG
                     VLYLDLLTNLERISDHSTNIVKQVLKLKQKI"
     gene            complement(12756..13496)
                     /locus_tag="L3M60_001915"
     CDS             complement(12756..13496)
                     /locus_tag="L3M60_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226581.1"
                     /translation="MKDFKDDIELIINDFFDCCNIPTKVFSKDLKEICSIGYDPTLET
                     YFSSLNIFSNINSTDFISNKNLFYIMLSFCDDLHFLVMPIHKFDVSSGYFIVGPFKSC
                     INNDIEFSNIPFKPLSCLDYISNLLSEICLDKMKHRPALSFYVKKTIDYIHKNYSEDI
                     TVSDICNDLQLNKSYFCSLFKKESGYTFTNFLNKVRVEKSKKYLLEKDLSILDVAVLV
                     GFNSQNYYSMVFKKFNNLTPIEYKNIYN"
     gene            13645..14394
                     /locus_tag="L3M60_001916"
     CDS             13645..14394
                     /locus_tag="L3M60_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6226582.1"
                     /translation="MKKIAIYGKGGIGKSTTTSNLSASLSHLGYKVMQIGCDPKSDST
                     KNLMKGKFIPTVLDVLKEKKEELKLEDIVFEGYGGVLCIEAGGPTPGVGCAGRGIIAA
                     FEKLEELKAFEIYNPDVVIYDVLGDVVCGGFSMPIRNGFANEVYIVTSGEMMSMYAAS
                     NISLAVDQFKNRGYASLKGLILNAKNVENERELVEKLADEIKSEVFHYIPRNKIVQQS
                     ENNGRTVIEEDENNEMSEVYIELANKIMKSN"
     gene            14436..15326
                     /locus_tag="L3M60_001917"
     CDS             14436..15326
                     /locus_tag="L3M60_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226583.1"
                     /translation="MKLKKLCSVFLLSSLILTGCSKASDGEAKEINNNVNVVSATVAA
                     TQVLDKLDATLVGIPTTKSDLPEKYKNLPEVGQAMNPDLEIVASLKPDVFIVDSMFKE
                     NVEKSMKEYNLDTFYFETGTYTQFLKSIEDLGEKINKQSEAKKLIDELEGVEQSVAEK
                     AKKQDKKPKVAILFGGGNNFMLATKSSYLGDLVKTVGAENITDNLTDNVESDYIQFSL
                     EQILKENPDYILRFAHGNIEETKKAFDDSFDKNPAWATLDAVKNGKVIDLDSSIFNVS
                     ANIHVKDSINKLGDILYGDK"
     gene            15343..16323
                     /locus_tag="L3M60_001918"
     CDS             15343..16323
                     /locus_tag="L3M60_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBN6226584.1"
                     /translation="MDNKKILLIVTGLITIIVLLILFSTIGSVNLSLREIFDALINEN
                     NPMVTTIVYKMRLPRNILAALVGANLAVSGLLLQAVMKNPLADPGITGISTGASVAAI
                     IILLLFPQYTNILPIVAFIGGLISCAVVYLMAYKNGLRPERIILAGVAVNTILGGVIS
                     YLSTMYSERIQSAMLWLNGSLATKTWLDVNMLIWYSIVGLIGAIFLIRSANVLQLGDD
                     AATNLGFNVNKSRFIISLVAIFLAGVSTSIVGVISFVGLIVPHITRMLLGSDHKYTIP
                     FSIILGSIVLLIADTLARTIGGSIEIPVGVIMSIIGGPFFLYLLRKRGSY"
     gene            16323..17117
                     /locus_tag="L3M60_001919"
     CDS             16323..17117
                     /locus_tag="L3M60_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226585.1"
                     /translation="MFNVKNIKIGYEDKIIIDNLSVSIKKSEIVSILGPNGSGKSTLL
                     KSLSRILKIKQGDIYIGKKNMKVMSNKEISRQVALLAQHNVSLKDIKVKDLIYYGRMP
                     HKGFFEGKNRNDDEIVTWAMRNTGLESYKDKLVSNLSGGERQRVWLAMALCQKPDILL
                     LDEPTTYLDISHQLELMELVRDINKKFKMTIIMVLHDINQASKYSDRLIIMNNGSIVA
                     DGHPNKVISEKIIKEVYKVKCDIDIDPISKKPRIHPIMLCRRTSSI"
     gene            17216..18493
                     /locus_tag="L3M60_001920"
     CDS             17216..18493
                     /locus_tag="L3M60_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
                     /protein_id="HBN6226586.1"
                     /translation="MYTLDVLNRLKDIDCDKDIKSLSYAVFPGTHCPLFGVVLTASYI
                     RNMALVVVGTNECTYYSKNFAYHRQFGNDSVYSVVLKDKDIVFGAEKKVVEAVKYISE
                     IGEFDAIMIVTTCVPELIGEDYNALSSELEDMLRIPVLTVNTEHYTCNSHILGMTRAL
                     RSLSSVMESYKKKEGVNILGHRQENVEDTELVKLLVKKDIKINCVIPSKCTIKDIKNA
                     SSAKLNIVTDMIALDLAEDMKNKFGIEYVYFDKNMKESIIIENYNKLCKILDIDIQDE
                     VSKQRQRYNSLLNECRYLLDGKKLIYGNTPMMAFETVDFLTELGLVPEFIQVRELYEQ
                     DNLFKDNIIKKGYNPYVSRIANIAPLRSLYENINADIYIGHENPMLLREKGLMQITLD
                     EHAQKIGFELPIGIMKTLIKLFDVEKSTRREVI"
     gene            18495..19439
                     /locus_tag="L3M60_001921"
     CDS             18495..19439
                     /locus_tag="L3M60_001921"
                     /inference="COORDINATES: protein motif:HMM:NF012375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HBN6226587.1"
                     /translation="MEIYRYFPNPSDRMGIIFIVSSINEACVIEFGPSGTTHYAIEAI
                     GSLNGEDKAKIYSTHMSESDVTFGNYDRLEKAIIEVDSNIKPKYIFVMASSVSSIIGT
                     DIIGICNILKESVNCRLIPITTGGLRDDYNQGVEEFLYILAKEVVKESSEKFDSYNII
                     GCTIDQFNFLADCEEIKRMMKAFFKKEVNVTFTSYTSIDEIENASKSSLNIVLRKEGI
                     KAAIFMKEKYSIPYVYKKPYGIKNTEEFINEIQKVTEWDLDTNTYDDEISNIKRYIFN
                     VKRKLYFYEGSKKCAVFGDYDTALGFRDLLEELGLKID"
BASE COUNT         7352 a         2277 c         3092 g         6736 t
ORIGIN      
        1 actaaaaaat gcaagtaatt gactttttaa gttgacaaaa tattgcatat atattatgat
       61 ataaatcata aattaactac aaatcaatat tatatattcg tagaagaaga aagtagcata
      121 aaggaatttt acagagagtt agatttggct gagaactaat attggaattt atgcgaaaag
      181 gactttggag aatactattg taactatgtt ataatagtgg tttaattcgc cttaagaaaa
      241 tgagtgagta aaattaattt ttactaataa gagtggtaac acggatttat attcgtctct
      301 tggatttttc caagaggcgt tttttattat aaacttattt cagaaaattt aaaactatat
      361 attaaatgaa aagtataaaa atatgatagt taataaattt ataaattaga aaattgtaaa
      421 atatactaaa gttagtatct ttacgattta aatggaggga tgaaaatgga agaagaaaaa
      481 attaactatg ctctggaaca gattccagag aatcaaaaac gtggttgggt agctatgttt
      541 tcagttttag tagcaatagg tgtggattta tcatctgtta ttttaggagc tgagttggca
      601 cagagtatgc caatgaaaca agcaatattg tcagtaattg ttggttcatt tttttctgca
      661 attttgtata ctacatgctc tttggttggt tcatctacaa gtctatctac atctatgata
      721 acaaagtatg tatttggaga agctggagca aaaatatttt ctctagttat aggagtttct
      781 ctattaggat ggtttggagt tcaagtaggt ttctttgctc aaaatgcaca gataataata
      841 aaagatatat ttaatttaga tgtgagtatg cagattttaa gcctaatagg tggtctcttg
      901 atgatgagta ctgcaattta tggatataag gcaatggaaa agttgagtgt gtattcagta
      961 ccgtttttat tagttcttat gatgttaact atattcttag cttttagagc aaatggaatt
     1021 ccagttgatg ataatatgaa atctactatg acatttgcag gtggagtttc tctgtcaatg
     1081 agtataataa tcgttggtgc aattgtatct cctgatataa gtcgttgggc taaaagtaga
     1141 agagactgtg cgctttcaag ctttttagga atacaatttg gaaatgcttt tatgataata
     1201 gtttcgatag tattagtaaa atgtatggga acttcagata ttatgagaat ttttattaca
     1261 ttagggattg ctataccagg tataatagta cttacattag ctcaatggac tacaaataca
     1321 agtaatgtat attcagcatc attaagtata gctcttgtac ttaagaaggc tcctgagaaa
     1381 gtattaacta tagtccttgg tattgtagca acattattgg ctgtatttgg aatatatgaa
     1441 ggttttatag gatttttaaa tttacttgga attgtaattg cacctgttgg tggtgtatat
     1501 acagcagaat attatatagt aaagcaagaa cttaaaggct ttgataaagg tgtattatat
     1561 aaaccaatag ttaaacgttc aatagtgtct tggataatcg gtatattaat aacttacctt
     1621 tcaacatatg gatttataac tcttactact atagcacctt tagatggttt tatagcaggg
     1681 tttgtagtac aatctattat tggaaaagtg ctttgctcaa tgaaaaataa tcaagaaata
     1741 gacaaagcag tataaagaaa tttgtttata aagtataaaa taaattagaa atatgaggag
     1801 aaagtaacat gagattttta acagaagaaa gtgtagataa aatagcagtt ggtgcagccg
     1861 tgcttggaac tggaggtgga ggtgacccat atgtaggtaa gttggttgca aaacaagcca
     1921 ttaaaaagta tggtcctgta aaagtaataa gcttagatga acttgatgat gatgctttgg
     1981 tagttccagt atctggtatg ggttctcctg taattacaat agaaaaatta ctttcagaag
     2041 tagagttaac tacaccttta gaaataatgg agaaattatt aaatagaaaa gtggatgtta
     2101 taattcctat agaaatagga ggaataaact ctcttatgcc gatagcagtt gcagctaaaa
     2161 aaggacttcc tattttagat gcagattcta tgggtagagc ttttcctgaa gctcaaatgg
     2221 tgacttttta tcttgaagga tatgaggcat ctcctgctgt aatgtctgat gaaaaaggaa
     2281 attctgctat tctttatcct gtagatggta tttggtcaga aagattagct agaactttaa
     2341 ctgtagaaat gggaggaagt tcttctatta gtgactataa tttaagtgga gctcaagtta
     2401 aaaaagctgc tataggtaat actcttacaa tagctgaaac tataggagga tttttacttg
     2461 aaagtaaatc tgattcaaaa ggtgctgttg agaatatact aaaagaattg agtggataca
     2521 aattatttga aggaaaagta attgatatta agagagagtt gaaaggtgga tttactagag
     2581 gacatgcttt ttttgctggg attaatgaat atgatggaga gtattctata ttattccaaa
     2641 atgagaactt aattgccaaa aaaggtgata caccactttg tattacacca gatttaatag
     2701 cagtgttaga tttagaaaca ggatttccaa taactacaga gagaataaaa tatggctctc
     2761 gtgttatggt agtagctttt ccttgtaatg aaaagtggag aacagaaaag ggaattgaga
     2821 cagttggacc tggatacttt ggatatgatg tggaatataa aacagtagag gaattacaag
     2881 gaaaataaaa gtttaggagg aaattttgat gtatcgtgta ggaattgacg ttggaggaac
     2941 aaatacagat gcttgtatac tggatgggga attaaaagta attcattcag ttaaggtggc
     3001 aactacaaag gatgttgaaa ctggtgttta caatgcctta aaaaaagtta tagatgaaag
     3061 taaaattgac cattcattaa taaaatatgc tatgcttggt acaactcatt gtacaaatgc
     3121 tattgtagaa agaaaaagac ttaataatgt ggctatgata agaataggaa agccagcaac
     3181 tactgccatt cttcctttta tagattggcc agaggactta agagaaaaaa tagagttaga
     3241 ttcaatattg gtatctggtg gatatgagtt tgatggtaga aagataaatg aactttgtaa
     3301 agatgaagtt gtagaatttt gcaatagaat aaaagacaaa gtggaaagtg ttgcaataag
     3361 tggagtattt gctccagtaa ataaacaaca agaagaagaa gttgcaaaat gggttagaga
     3421 aattctaggg gacattccca tatctttatc aaatgaaata ggtaatatcg gtatattaga
     3481 aagagaaaat ggggctattt taaattctgc attgtctcaa gtaggtaaag ctgtatcttt
     3541 aggatttgaa aaagcactta aagatttagg catagacgct caaatatatt ttagtcaaaa
     3601 tgatggaaca ttaatgaatt tagagtatac aatgaaatac ccaattttta caattggatg
     3661 tggaataaca aactctataa gaggagcttc ctttttatca aaagtaaaaa atgcaatagt
     3721 actagatgtt ggaggaacta caagcgactt gggtgtacta tacaatggat tccctagaga
     3781 atcatcaatt ccagttacaa ttggaggggt acatacaaac tttagaatgc cagatgttct
     3841 aactattggt cttgctggtg ggacatgtgt aaaaggcgaa aaggataata taaagatagg
     3901 tcctgaaagt gtgggatata gaataactga agatgcaata atatttggtg gaaacactct
     3961 aactttatca gatattgtta caaaattaga tatggcattt gaagatagaa aaagcaatgt
     4021 tgaaaactta gataaagagt attgtgatga ggtatataag aaggttattt taaaattaga
     4081 agatgctgta gaccaaatga agactagtca agatgatgtt gaattggttt taactggcgg
     4141 aggaagtata attgttcctg aaaaactaaa aggcataagc aatgttataa gacctcctca
     4201 ttatactgct gcaaatgcta ttggtgcagc tttaggacaa ataagtggag atgtagaaaa
     4261 agtatatagt ttagataaaa tgtcgagaga agatgccata gaagatgcaa aaaaagaggc
     4321 tgtaaataaa gctctaaaag ctggtgctaa agaagatact atagaagtat taaacataga
     4381 agatgttcca cttgcatacc ttcctgggaa tgcacttctt ataaaagtaa aagttgttgg
     4441 tgatttaatt taaagaataa atataagtaa tttcagggtc tttaaaataa agtgtgtatt
     4501 cttctaaaaa gtggtgataa ttaaaactat taaatatcac taatacaaag gaaggtgcac
     4561 actttttatg tttaacaaaa acgaaatata aaaaaaactt attgaagaat atgatgtaaa
     4621 gacaactaca gatattcaag atatgctaaa agatttattt gcaagtacaa ttcatcaaat
     4681 gttagaagca gagcttgaca agcatttaga ttatgacaga tacgataata agaacaaaaa
     4741 tactaaaaac tctagaaatg gatatgaaca gccgatatat acctattttt taaggtttat
     4801 tggctgtttg cttttgacag gtagaccata caatttatac aaattcttat atttatactc
     4861 taaatcatta tacattttat tgcaaagcat ataatcagac ttagcatttc tatatttgct
     4921 tttacatctg gcatataagt gattccattt gtcaactaac tggattttgt actttttata
     4981 aaaaactatt ttatatagag atttctgtct tttatcgcat tccaactatt tttcttactg
     5041 tatttgctgt acgaattgta atcttatcac ttacatttga actagcagtc tttgaccacc
     5101 aatttgtctt ttgataatct ttacgactaa aagaccaaaa aatagcattt ttatagtgat
     5161 acactttttc atattcttct ttaggatttc caacctcatc ataaaacttg tcataagata
     5221 agtcaggaaa cataaaaata agattatcat atatttcttt gttgttatct ccccaccaac
     5281 taggagcatt atttagtata tcctctaaca cctcttgtga aataataaaa actggtatct
     5341 ccaattcaaa ctgttttttt atcattaatc ctattgaatt tgagataacc tcgctgtcat
     5401 ctacattact taaaaagaca acattgccac tattaaggta tgtagaaact gctgaataac
     5461 caagttctac aaaccctgct tttaattcag acataactat tttgttctta ccacttatat
     5521 tgatacctct taataatgcg atataccttt tcatataata atttcacctc taaacattta
     5581 catttttcta tgtagcacaa actggaattt gtatctttta taattttttt aaagcttttt
     5641 tatatgtaat ttccccaagt ataaactgtt ctaaagcatt tatttttttc agattcagta
     5701 ttgtattttt caacaattct tctaataaat ctatttcatt caaaatcggt ttataaatat
     5761 gtttacaaca attaaattct tcaaagtatc ttgaaccaaa tcatatttga tattctacat
     5821 tgatatggat ttgaaaataa ggtatctcct gtgataaaat aacagttttt atgtttttta
     5881 acatattttt gaagttcttt attaatcaat ttacattctg tacatccttt atcaaataca
     5941 aatttacata ctttttttaa tataaaaaat cggtgcctaa tttgtaccga tttttgtttt
     6001 tcttagtaaa gaactcgctt ttttgcttct gctatagccc tagcagcact ttttgcttgt
     6061 attctttttt cttcttctct tttaaattta tttattgttt catttagatt cttatcatgt
     6121 tcatttttct ctttatcttc gttttctctg tatactaaca cataatctat tattgattcg
     6181 tttattatct gggatatact tttattttca ttaattccta ttctcttaac ttccttaagt
     6241 agagtctcat caatatgaac cgttgtttgc tttgtatttt ttgccacttt aatcacctct
     6301 taaaaaattc taacattaaa attttttttg gtcaataaat tttaatatta aaattcttta
     6361 aatgttttat ttgattttct aaaattttct attgtatcta tcatcttatt ttttatattt
     6421 tcacctaata cagaagactt tagtctagga ttttcgatat ggtcataaat tggttctaaa
     6481 aattcgactt ctacaggaac agaattaatt ttaccaatag gttggtatcc ttcataagta
     6541 tcttttgaat tttttattac tagtggaaca atagggcatt ttgctttata tgctatcttt
     6601 aaagctccac ttctaaactc tgaaaccaat gaatttggtt ctttaatcca tgttaaatct
     6661 ccttctggaa acactgccat actttgacct gtaagtatat tttgagaggc ttcggctata
     6721 ctctttatac cctcacgatt gttttttctg tttacatata cacatcttag taattttgcc
     6781 cattctttaa atataggtat atttctccaa actggttcat ctgcaattac acatccaatt
     6841 ggtctttcta cacttgctgt tagtatgaag ctatcaagca tactagaatg atttacaaca
     6901 aatagaagtg gttcttttgg caatgtttct ttaccaataa tattaagttc tatattcact
     6961 atatctaaag attttttagc ttgtttttgc ataaattcaa atttttcttt taagctaaat
     7021 ttatcaggat tctttttaat tttaatgagc ctaggtatag aagatgtaaa acctataagt
     7081 tgatatgtag caaattttaa gtaattattc ataagattat ctccttaaaa taaaattatt
     7141 tattactgta aagattttaa cataaaataa aatttaagtc tattttaatt caattttcta
     7201 acagatattt caaatacaca gagattaaga ttttatattt caaattttag taatcagatt
     7261 ttatcaataa gttactattc tattaatgta attaaataac ctttaaattt aattacaaaa
     7321 actgttgcag atttttctac aacagcttaa aagacttatc ttataaaaac ttattttata
     7381 ataagaaagt atattataag gctaggacag cccatttttt acactgaaca attcttttac
     7441 catcatcacc ttgttctttg tcatatgcaa attgattaag catttcacat ggaaatgtat
     7501 cacatagacc acaatgtgta aggtttttat tttcacaaca agattttaca ggacattgtt
     7561 caccccaaaa tggtttctta atatctgtac aacctttaca attgactttt tccttgtact
     7621 cacattcata acataaaatt ccacatcttg attctatcat tctaagtcac tccttaaata
     7681 taaattttat atgattgtat atcaaaattt atacttttca ttgtaaaaat cggacagatt
     7741 ttttatatag gttgttggat taacaccaca aatagattta aaatcattta taaaatggga
     7801 ttggtcgtaa taaccaatat cttgtgctaa agttacaaat ggtattttat tattttgtat
     7861 ttcttgcaac acaaggttta tacgaagcag tctaaggtat gatttaagac tcataccaag
     7921 agaattatta aaaattctat ttaaatgtcg ttcactataa caactaattt gggaaatgct
     7981 tttaacagac ataatagagt tttgttttct ggcattttca agaattggta tagtcatatc
     8041 tactatattt gctttcaaca agtatgataa aaataacata tctaattgtt ctactaattc
     8101 ttttatagta cttgttcttt caaaaataga attaacctct aagctaaata atgtgttaaa
     8161 atcttcaagt gaaattttta aatctgtaga ttctctttga gaaagacctg tcaaataata
     8221 tactccacct gggcgaaatt caacaaatac tctaaaaagt acattttcta tatcattttt
     8281 cacaatagtg gtttttgttg tagctcccca gaatgaactt tctatacctt ttttatcaaa
     8341 tttaaatatc atacatccac tagcatcagg tactaatgta agattttctt taatacttgt
     8401 gtctggaata gatattgtat agtgtgctat atactgtctt aacaatttat gtggcaaaat
     8461 atatgtatac tgtttgcagg tatttaaaat gttattgatt attttattca taagtatatc
     8521 ctcctgagat ataaaaaata agtatactaa tttacttttt atataataaa ccagttttga
     8581 tttaattgaa atattttaat aagatatttt gttaaattta gatacaataa ttgtaaggta
     8641 aaatctcaaa atatacaatt attataaatc ataaatattt atcaaataaa tatattaaag
     8701 aaaagtagat ataaaacaaa attagaaaat aatagtgtta ctttcataat aaatacatat
     8761 aaagatattt ttaagaataa aatttactat taaataaaaa ataaaggaga gtgatttaat
     8821 agtatggcat caaaacaaac accaagaaac aatttggaaa agtttttaag aataagagtc
     8881 ttatcacata caggagataa aatacatata aagttacctg taaattttgc aaagagaatg
     8941 attgagaata atgctttgga tttatttaat ggaaaagatg atgtagtaga tggtaaaaaa
     9001 gttactgaaa tattagtcaa agcatttgac tatgatttaa ctggagaagt agttcactta
     9061 gagagaaaaa atggagatat tattaaagtt tcaatcgtac aatagaatta ttttatatga
     9121 attttatttt taaatagtta taaataatta gaaatttgta aaaaagtata atatattatt
     9181 agtaatgatg tagaatacac atttataatt tatagatgtg tattttttgt ttgaaaaatt
     9241 taaatatata aaactataaa ggttacaatt ttggaaatag atgtaaatat atgttttata
     9301 gtataataag aagtaatata ccatctagta aaaataatta aataaattta atttatgaga
     9361 gggataacta tggaaaataa tgattttaaa ttaacaatat gtatatttgc ttttgtaact
     9421 ggagtagtag tattattctg ctgtataggt ttaggtgaat ttagtgtaga gcatattatt
     9481 caacaaaatg gtggaaccat ggatactgac caatataata tttatctaga ccaatctatt
     9541 tctcaatata gaaattttgg ttctatatta gcgttgttag gaggactagg gatattgttt
     9601 gataaatata tcagaaaata ctaaagatta tgttttgttt aaaataaatt tttatgagga
     9661 gataaaaaat ggcaacagaa atatttactg taaaaattac gcctatagaa gcacaaaaaa
     9721 taataaaaga aaatcttaat gcagatttag tttttagtga ttcttataat ttaggaaatg
     9781 ataaatttat actgattaca acttttgaga agtattatgc aagaaacaat agtgatgcag
     9841 ggttaatcgt agtttatgaa aatacaacag gaaaaacagt tattaaagct atttcaacag
     9901 gtagtgcaat tggagtttta aaaattgact ggggtgcagg tggaaattta cttaaaaggg
     9961 taaaaaatat acttgctgat tatattattt aattatttta tctaattgat aacatgtcaa
    10021 gaaaatctcg taaaagaggt taagaacatt cttataatat atagataaac aagaatttaa
    10081 aggtaaagct tttaatctat aataaataat attaatatag tatgaataaa aagtaggagc
    10141 atattaatat aatgccccta cttttttatt attaaaattg tttataatat taaaactaca
    10201 ttaaaataca gtctaaattt aacaaataaa tggtgtagaa atatgtactt tttttaattt
    10261 attatattct tttaaattga attttttcat ctgaacataa agtctcaaat ttgtatgcgt
    10321 ctgtcatatc ataaaaacca aaaaatataa agccatcacg agaagtaaat tcatattgta
    10381 tattttcaat tttcaatata ttttctacct cacttatttt ttcactatat ataatttcta
    10441 tcatataatt tatcattgta gaatccctcc tattaatatg aaaatatcca aatatataat
    10501 aacatattta gatgatataa tagtatccaa ataaaaaata aagttatttc attattgaat
    10561 gaagaattaa aatgaagata aataaagtaa atattggtta taatagatta aaaaatggct
    10621 atgtacactt gtaattttag tcataggagg ttaaagtttt atgaaagaag tcgtatatat
    10681 atggggtatt ctagtcagga attgtttaat aataagataa tactataaaa atttgagtat
    10741 tatataaaaa gtaagaagcc ataaatgata taagcattat ctatagcttc ttatttctca
    10801 ttgattaaga ttatataaaa acaataacta tattttttgt ttaagtttta atacttgttt
    10861 tactatattt gttgagtggt cagaaattct ttctagatta gtaagtaaat caagataaag
    10921 tacaccagca tcaattgtac aacttccgtt atttagtcta atcatatgtg aatttttaaa
    10981 agttttttct aaataattta cttcattttc aacttctaaa actttatttg ctaactcaaa
    11041 atctttgaca tctaaaacta ttaaggcatc cttgaaatta gtaattgttt tattatatat
    11101 ttcatatatt tctcttattg cactatcaga aaaactaata ttcatttcct taacactcat
    11161 ggccaattca gctatatttt ccgcatggtc agagactctt tcaatgtcat ttacagtatt
    11221 aaaaagtaaa tctgttctaa aacgttcatc atctctaaga ggttctttag aaagttttaa
    11281 taaatagttt aatatatctt tctgtaactt attgacaact ttttctgatt caaaagcttt
    11341 attaatatcc tcattgctct tatttaaaaa tcctgcaatt gaatgttcca aagagtttgt
    11401 agctttatga cccattctta caacttcttg tacagtatta ccaagtgcta tagatggagt
    11461 actaaacatc ctttcatcta agtatttagt gttatttact atttctgatt cagtttcttt
    11521 aataggtata agtctatttg ctaatttaac tatgtattta gaaaatggta ataatattat
    11581 aacatttgta agattaaata aagtatgtgc atttgctatt tgtctagctg tatcagttgg
    11641 gtcaaaatga gtaactatag ctgatatagg tttatttaaa ataagcataa atattattgt
    11701 accaatgaca ttaaaactta agtgcattac agctgcacgt cgagcatttc tacttgcacc
    11761 aacacttgat aaaagtgaag taacacaagt acctatgtta tctccataaa gaataggcaa
    11821 agcagatgat agaggaagta ttccttgaga agctaatgct attaacatac ccatagatgc
    11881 acttgaactt tgaacaatac cagttatggc gaatcctgcc aatataccta atataggatt
    11941 tttactaaaa tataaaagtg catcagtgaa tgctttatat tcagcaagtg gagctactgc
    12001 atctttcata aactccattc ctgtaaataa tataccaaaa cctataagta tttcagataa
    12061 atgctttgtt ttttgatttg atgtaaataa atagaataaa atacctatac ctagagcaat
    12121 tggtgcaatt ccttcaagat taaatgaaac taattgagca gtaactgtag tacctatatt
    12181 tgcccccatt attacaccaa tagcttgact tagattcatt atacctgcat ttacaaatcc
    12241 tacaaccata acagtagtag cacttgagct ttgtataata cctgtaacaa ctgtacctac
    12301 tagtacaccc ataacaacat tacttgttaa gagttcaata atttttttta atttgtctcc
    12361 tgctgatttt tgtaaacctt ctcccattaa actcattcca taaagaaaaa gtccaagtcc
    12421 accgataagg ctaattacta tattcacgat atttcctccc ttaagaaatt aatgattttt
    12481 ttatctgaat tataaacttt attaactaaa atttattgtt tccataatat agaataacaa
    12541 attttgtcaa taagaatatt aatgttttgt taagtatacg ttaagagaat gttaaatttt
    12601 taagttgttg ttaattttag aaaatttatg atgtatagca atttaaatac ttggacttag
    12661 atttttagta ttttatgtac aaacaattta acaaagaatt aactataatt gtaaaaaata
    12721 tgtaaaaaaa cagcatcttt ttagaatgat attttttagt tataaatatt cttatattca
    12781 attggtgtaa ggttattaaa ctttttaaaa accatactat agtaattttg actattgaat
    12841 ccaactagta ccgcaacatc taaaatagac aaatcttttt ctaatagata ctttttgctt
    12901 ttttcaactc taactttatt taaaaaatta gtaaaagtat atccactttc ttttttaaac
    12961 aaagaacaaa aataactttt attaagttgt aagtcattgc atatatcact tactgttata
    13021 tcttctgagt agtttttgtg aatataatct atagttttct ttacataaaa actaagcgct
    13081 ggtctatgct tcattttatc aaggcatatt tctgataaaa gatttgatat ataatctaaa
    13141 catgataaag gtttaaatgg gatattagaa aactctatat cattattgat gcatgattta
    13201 aaaggaccta caataaagta accacttgat acatcaaact tatgtatagg cataactaaa
    13261 aaatgtaagt catcacaaaa acttaacatt atatagaata gatttttatt agatataaaa
    13321 tctgtactat ttatattgct aaatatattt aaacttgaaa aatatgtttc taaagtaggg
    13381 tcatatccaa tactacaaat ctcttttaaa tccttactaa atacttttgt tggaatatta
    13441 caacagtcaa aaaaatcatt gatgatgagt tctatatcat ctttaaaatc tttcaaaaaa
    13501 tcacctcatt taaatttata caaatatttt tataaaaaaa caaatatagt taagaataaa
    13561 aaaaatatat caattaaaat agttattgcg aatgataatt aatttcgtta aataatatta
    13621 tgatgtaaga gaggtagaaa gtcaatgaaa aaaatagcta tctatgggaa aggaggaatt
    13681 ggaaaatcaa caacaacttc caatttatcg gcatcacttt ctcatttagg atataaggta
    13741 atgcagatag ggtgtgaccc aaaatctgat tccacaaaaa acttaatgaa aggaaaattt
    13801 ataccaacag ttcttgatgt attgaaagaa aaaaaggaag aattaaagct tgaagatata
    13861 gtgtttgaag gttatggagg agtattatgt attgaagctg gagggccaac tccaggtgta
    13921 ggatgtgcag gaagaggtat aatagcagct tttgaaaaat tggaagaatt aaaagcattt
    13981 gaaatatata atccagatgt agtaatttat gatgttttag gagatgttgt ttgtgggggc
    14041 ttctcaatgc caataagaaa tggatttgca aatgaagtgt acatagtaac ttctggagaa
    14101 atgatgtcaa tgtacgcagc aagtaatata tctttggctg tagaccaatt taaaaataga
    14161 ggatatgcct ctttaaaagg gcttatatta aatgcaaaaa acgttgaaaa tgaaagggaa
    14221 ttagttgaaa aattggctga tgaaataaaa agtgaagttt ttcattacat accaagaaat
    14281 aaaattgtac agcagtctga gaataatgga cgaactgtta tagaagaaga tgaaaacaat
    14341 gaaatgtcag aagtatatat agaacttgca aataaaatta tgaaaagcaa ctaaaaaagt
    14401 atatttaaaa ttaagtttat tatggaggaa attttatgaa attaaaaaaa ttatgctcgg
    14461 tatttttact tagctcatta atattaacag gatgctcaaa agcatcagat ggtgaggcta
    14521 aggagataaa taataatgta aatgttgttt cagctacagt ggcagctaca caagtattag
    14581 ataaattgga tgctacgttg gtaggaatac caactactaa atctgattta ccagaaaaat
    14641 ataaaaactt gccagaagta ggacaggcaa tgaatccaga cttagagatt gtagcatctc
    14701 taaagccaga tgtatttata gttgatagta tgtttaaaga aaatgttgaa aaaagcatga
    14761 aagaatataa tttagatact ttttattttg aaactggtac atatacacaa tttcttaaaa
    14821 gtatagaaga tttaggtgaa aaaataaata aacagagtga agcaaaaaaa cttatagatg
    14881 aattagaagg agtagaacaa tctgtagcag aaaaagcaaa aaaacaagat aaaaaaccaa
    14941 aagtagcaat cttatttggt ggtggaaata attttatgtt agctacaaaa agttcatatc
    15001 ttggggattt agtaaaaaca gtaggtgctg aaaatataac tgataaccta acagataatg
    15061 tggaatcaga ttatatccaa tttagtttag aacaaatttt aaaagaaaat ccagattata
    15121 tacttagatt tgcacatgga aatattgagg agactaaaaa agcatttgat gattcttttg
    15181 ataaaaatcc tgcatgggct acattagatg cagtaaaaaa tgggaaagtt atagatttag
    15241 attcaagtat atttaatgta tctgcaaata tacatgttaa agattcaata aataaattgg
    15301 gagatatctt gtatggagat aaataaattt taggtgaata atatggataa caaaaaaatt
    15361 ttattgatag ttacaggttt gataactata attgttttgc taattctttt ttctactata
    15421 ggaagtgtaa acttaagcct tagagaaata tttgatgcac tgataaatga aaataatccg
    15481 atggttacta cgattgtata taagatgaga ttacctagaa atatattggc agcattggtt
    15541 ggagcaaact tggctgtttc tggtctatta ttacaagctg ttatgaaaaa tccattagca
    15601 gaccctggaa taacagggat atcaactggt gcaagtgtag ctgctattat aatattgctt
    15661 ctatttccac aatacacaaa tatattacca atagttgcct ttataggagg tcttatatct
    15721 tgtgcagtag tatatttaat ggcatataaa aatggtctaa gaccagaaag gattatatta
    15781 gctggggtag ctgtaaacac tatattaggt ggagtaatat cgtatttatc aactatgtat
    15841 agcgaaagaa ttcaaagtgc catgctgtgg ttaaatggga gtttggcaac aaaaacatgg
    15901 ttagatgtaa atatgcttat atggtattcc atagtaggtt tgataggagc gattttcctt
    15961 attaggagtg caaatgtact acaattggga gacgatgcag ctacaaatct aggtttcaat
    16021 gtaaataaga gtagattcat aatatcgtta gtggcaatat ttttagctgg agtttctact
    16081 tctatagtag gggttattag ttttgtaggt ctaatcgtac cacatataac gcgtatgttg
    16141 cttggtagtg accataaata taccattcca tttagtataa tactaggaag catagtttta
    16201 ttaattgcag atacattagc gagaacaata ggaggttcta tagagattcc agtgggtgtt
    16261 attatgtcta taataggagg tccattcttc ttatatttat taagaaagag aggaagttat
    16321 taatgttcaa tgtaaaaaat attaagatag gttatgaaga taagattatc atagacaacc
    16381 taagtgtaag cattaaaaaa agtgaaatag tctctatatt aggaccaaat ggttcaggaa
    16441 aatctacact tcttaaatca ttgtctagaa ttctgaaaat caaacaaggt gatatataca
    16501 ttggtaaaaa aaatatgaaa gttatgagta ataaagagat ttcaagacaa gtggcattat
    16561 tggctcaaca caatgtatca ttaaaagata taaaggtaaa agacttaata tattatggga
    16621 gaatgccaca taaaggtttt tttgaaggta aaaacaggaa tgatgatgag atagtgactt
    16681 gggcaatgag aaatacaggt cttgaatcat ataaggataa gttggttagt aatttatctg
    16741 gaggtgaaag gcaaagagtg tggttagcaa tggctctttg ccaaaaacca gatatacttt
    16801 tattggatga accaacgaca tacttagata tatcacatca attagaactt atggagcttg
    16861 taagagatat aaataaaaaa tttaaaatga ctataattat ggttttacat gatataaatc
    16921 aagctagtaa atacagtgat aggcttatca ttatgaataa tggttctata gtagcagatg
    16981 gacatccaaa taaggtaata agtgaaaaaa taataaaaga agtatataaa gtaaaatgtg
    17041 atatagatat agacccaatt agtaaaaagc ctaggattca tccaataatg ctatgcagaa
    17101 gaactagtag tatatgaaag tataatatta taaataatta aaaagcaaaa gttattatct
    17161 atgtaaattt ataagtttga atttatatag ataatagaag tagaaaggaa aatatatgta
    17221 tactttagat gtattaaata gattaaaaga tatagattgt gacaaagata taaagtcttt
    17281 atcatatgct gtattcccag gaacccattg tccattgttt ggagttgtat tgactgcatc
    17341 atatataaga aatatggctc ttgtagtagt aggaacaaat gaatgtactt actatagtaa
    17401 aaactttgca tatcataggc aatttggcaa tgatagtgtt tattcagtag tactaaaaga
    17461 taaggacata gtttttggtg cagaaaaaaa agtagtagaa gctgtaaaat atatttcaga
    17521 aattggtgaa tttgatgcaa taatgatagt aactacatgt gtaccagaat taataggtga
    17581 agattataat gctctgtcta gtgaattaga agatatgctt aggatacctg ttttgacagt
    17641 taacacagag cattatactt gcaattctca tatacttggg atgacgagag ctttaaggag
    17701 tctatcatca gttatggaaa gttacaaaaa gaaagaagga gttaatatat taggtcatag
    17761 acaagaaaat gtagaggata cagaacttgt aaaattgctc gtaaaaaaag atataaaaat
    17821 aaactgtgta attccatcta agtgcactat aaaagacatt aaaaatgcat ctagtgctaa
    17881 attaaacata gtgacagata tgatagcttt ggacttagca gaagatatga aaaataagtt
    17941 tggtatagag tatgtatatt ttgataaaaa catgaaagag tcaattataa tagaaaatta
    18001 taataaactt tgtaaaattt tagacataga tatacaagat gaagtatcaa agcaaagaca
    18061 gagatacaat tcattattga atgaatgtag atatctgttg gatggtaaaa aattgattta
    18121 tggaaataca ccaatgatgg catttgaaac agtcgatttt ttaactgaat taggactggt
    18181 tccagagttt atccaagtga gagaacttta tgaacaagat aatttattta aagataatat
    18241 aataaaaaaa ggatataatc catatgtaag tagaattgca aatatagctc cacttagaag
    18301 tttgtatgaa aatataaatg cagatattta tatagggcat gaaaatccaa tgttattaag
    18361 agagaaaggg cttatgcaaa taacattaga tgaacatgca caaaaaatag gttttgagct
    18421 tccaatagga ataatgaaaa cattaattaa attatttgat gtagagaaat ctacaagaag
    18481 ggaagtgatt taatatggaa atatatagat attttccaaa tccttctgac agaatgggaa
    18541 taatatttat agtatcatca ataaatgaag catgtgttat agaatttgga ccatcaggta
    18601 caactcatta tgctatagaa gcaattggaa gtttaaatgg agaagataag gctaaaattt
    18661 attcaacaca tatgagtgaa agtgatgtta cttttggaaa ttatgataga ttggaaaaag
    18721 ctataattga agtagatagt aatattaaac caaagtatat ttttgtaatg gcttcatctg
    18781 tatcttcaat tataggaaca gatataattg ggatatgcaa cattttaaag gaatctgtaa
    18841 actgtagact aattccaatt acaacaggtg ggttgagaga tgattataac caaggcgtag
    18901 aagagttcct ttatatactt gcaaaggaag tagtcaaaga gagtagtgaa aaatttgata
    18961 gttataatat tattggatgt actatagacc agtttaattt tttagcagat tgcgaagaaa
    19021 ttaaaagaat gatgaaagct ttttttaaaa aagaagtaaa tgtaaccttt acatcatata
    19081 catcaataga tgaaatagaa aatgcttcta aatcaagtct aaatatagta cttagaaaag
    19141 aaggtataaa agcagcaatt tttatgaaag aaaagtatag tataccttat gtatataaga
    19201 aaccttatgg aataaaaaat actgaagaat ttataaatga aatacaaaaa gtaactgaat
    19261 gggatttaga tacaaatacg tatgatgatg aaatatcaaa tataaaaaga tatatattta
    19321 atgtaaaaag gaagctttat ttttatgaag gaagtaaaaa atgtgctgtg tttggagatt
    19381 atgataccgc attaggcttt agagacttat tagaggagtt aggtcttaaa attgattaaa
    19441 aaatagctac gcacact
//
LOCUS       DAFNNB010000063        19406 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0064, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000063 DAFNNB010000000
VERSION     DAFNNB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19406
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0064"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(2..466)
                     /locus_tag="L3M60_001922"
     CDS             complement(2..466)
                     /locus_tag="L3M60_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016208838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226588.1"
                     /translation="MVHLVYCDNAGKKGEKVLDKILAGTKTMVIRGAAGRKIPHSRVF
                     KDELLYFMEKGTAKISAKAIVKKVENYVKLTEDEISKILNENQPKLNLSEKQKVRWHK
                     KCLCLVEFENVEEITPLDFDHQGNMDDWLIIEKIEDVVVGTSIPYNYEKSKF"
     gene            735..1673
                     /locus_tag="L3M60_001923"
     CDS             735..1673
                     /locus_tag="L3M60_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6226589.1"
                     /translation="MKKIKSLAIFISIITTLVLVTACSDKNAEDKDKSETRVVQSVKG
                     EVKIPSNPKKIVDISGSSEELLLAGYKPIATANVDSYETDKLPSYIREELKDVKIVGH
                     SMMDTMDMEAILEVNPDLIIMSQRQEKIYDQLKEIAPVVMMKDYANDWRSKLTDVSRL
                     FDKEEETKSWLQKYDEKATKLGKEVIEKNGEKTYLPVLASSGQFMVFSDGGIGTLIND
                     DMKLARPKNMPKQDGITLPMISMEGLTDIDADHIVVIATESDKKDLENSAIWAQIRAV
                     KDGNVTILDSAPFFSQSYNPMGKELLLESVKNELTK"
     gene            1802..2815
                     /locus_tag="L3M60_001924"
     CDS             1802..2815
                     /locus_tag="L3M60_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBN6226590.1"
                     /translation="MTKVSKKNITYLLILVSIVVLVFGILLSITIGAKDMNLSTVIDS
                     LIKMEDGINIRIVRDVRLPRAIAATLVGGFLAVSGAIMQGITRNPIAEPSVIGITQGA
                     TFTISISLVLQKKLPQLIHGSFSVMMFAFIGASISGLFIYFISSKSRGRVNNVKLALA
                     GVALGTLLISLASAISMYFNLSQQLSFWISGGLVGVNWEGIKLLFIAGGIGFILAIIM
                     APRITILSLGEEVAIGLGQKTNIVRFVCIVLVILMTGASVSVSGNIIFIGLIVPQIAK
                     GIVGSDYKYIIPSSLVLGAVLLVYTDILSRMINPPYETPVGSITALIGVPIFIYLVRK
                     GEK"
     gene            2821..3843
                     /locus_tag="L3M60_001925"
     CDS             2821..3843
                     /locus_tag="L3M60_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HBN6226591.1"
                     /translation="MKNIIKINSKTLLVILILVVLIFITLLVSLNMGSFSIEPMDVIK
                     TFLGQGQRNHEIAIFKLRLPRIVIALLVGSALSTAGVILQGVTKNDLADSGILGINSG
                     AALFVVVYIYLMNGNVYEGMSNLTVFTMPIVALVGALFGAFLIYSLAWKGGINSSRLL
                     LIGIGVNIAFTSILTIFQLKFTTQEFNRVMAWTSGSVWGTSWKYVIAVLPFIVIFMGI
                     TIYKSKYIDVLNLGDEVSISLGVDVEKQRRRLIIYAVILSGVSTAVAGSISFLGLIAP
                     HIGRKIIGPKHKRLIPVSALIGTLLLLIADTISRNLLAPIEIPVGIVVSIIGVPYFIY
                     LMLVND"
     gene            3858..4640
                     /locus_tag="L3M60_001926"
     CDS             3858..4640
                     /locus_tag="L3M60_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004460318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226592.1"
                     /translation="MNCINTKNLNVSYGNTDIIKNLNLNIPKGKITTIIGANGCGKST
                     ILKTIGRIISPKSGKIYINGEDIYKKKSKEIAKDMAVLPQSPQAPSGLTVSELISYGR
                     FPHKSGFGNSSKEDREIVNWSLEVTGIAEFKDRNMDDLSGGQRQRAWIAMALAQETDI
                     LLLDEPTTYLDLAHQLEILKLLDELNKKQGRTIVMVIHELNNAARFADHMIGIKKGKI
                     VCEGDAYDVMTKDNLKELFNIDAEIATDPRTNKPICITYDIV"
     gene            4652..5992
                     /locus_tag="L3M60_001927"
     CDS             4652..5992
                     /locus_tag="L3M60_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6226593.1"
                     /translation="MENQEALRHEKIWILLFKYSIPAIIAMMVTSLYNVVDRAFIGSM
                     EGIGSIAIAGLGVTMPVFTLIIAFGMLVSVGASTRLSIKLGERNREEAEKILGNALTL
                     SIIISLIITILGLIFLEDMLFILGASKDSISYAKDYMSVILVGSIFNLVAFSLNNAIR
                     AEGNPKLAARTMIVGCVLNLILDPIFIFVFNLGIKGAAIATVLCQIVVFFWVTHYFMR
                     GKSNLKLKICNLKLNKDIVKKVFAIGMTPFAMEVAISITHMFTNNSLKVYGGDLAIGA
                     MTALTSILLMFMMPVFGLNQGMQTIISYNYGAKKYERAKKTLILSIIVSVVILSFGFL
                     VVQVFPEVFVGIFNKDPDLMNIAVRGININLITLPIMGISIVGPVYFQCISKVKHSMF
                     LTLLRQFILFIPLIIVLPIQFNLDGVWLAQPIADFIAMIIVLLFLKREFKTSQV"
     gene            complement(6083..8365)
                     /locus_tag="L3M60_001928"
     CDS             complement(6083..8365)
                     /locus_tag="L3M60_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6226594.1"
                     /translation="MNFENTKNKKIIATLLLTLLIIVSLTCVTFIMNLNKVTHNETER
                     YLIEISQSISTTIDVKCKSIFKTLYSVGDTYFEVNDSDKEVNKYLSKKLKLFEFDWLG
                     IINSNGNFISALKDTGNVKGQDFYIKALQGKETITPVKSPFGTYGILYSVPLYKDDKI
                     VGAVAAWNTIDTMRNLLSVESFNGEGFSQIIDQNGNFIVSSANKNAPKNVVNFFDMIE
                     HKGKQVDKNKLDEMKTNLKKNKTGSMYYTLDDGISKAMHYIPLQEENLYLLSIVPTKT
                     ASAQFDLLLKKAILINILIVILFLLLIILIMFINQKNRAQLIKIAYVDSVTKGYSKAR
                     FDMEAKKIIQSSSSESYSLVSINIQKFKLINDSFGSEAGDKTLKYIHDVISNYLKPEE
                     LLCRVSNDQFNVLIKTIPQKEILSYIENITNDINSFNKNEECKYFIALSIGVYKIDDP
                     NLPLVSIQDRANVARKNNKGNIGNHPLYTCVFYSDLERLKMIKEKEMENRMEEALNNN
                     EFIVYLQPKISLKDNTIVGAEALVRWQEPNKGLIPPDEFIPFFEKNGFITKLDHYVFE
                     KVCSMIRKWIDNGETPIPVSVNFSRANLNNIDFLEKYRMIRNKYDIPAKLLEIELTET
                     LVFENLQKLINIIDQIHEEGFQCSLDDFGSGYSSLNMLKEIKVDTLKLDRAFFSSPNA
                     DNIAENHIIESIVNLTKKLNMNSISEGVETILQMDYLKKINCDMIQGYVFSKPLPQEI
                     FEKMTFGKTLEETSMDSFVK"
     gene            complement(8695..9858)
                     /locus_tag="L3M60_001929"
     CDS             complement(8695..9858)
                     /locus_tag="L3M60_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBN6226595.1"
                     /translation="MKLMGYLRENGQFGIRNHVLVIPTSVCSSETATRIASLVPGAIA
                     IPHQHGCCQIGSDIELTAKTLIGFGKNPNVAAVLVVGLGCDGIQAKDLSSEIATTGKK
                     VDYVVIQECGGTLKTVSKGAEIVGKMAREVSKEVRVEFDMSEIILALECGGSDPTSGI
                     ASNPSIGVASNLLIDEGGSSILSETTEVIGAEHLLATRFEDGKMKDKFLKFVSDVEKR
                     AITMGEDLRSGQPTPGNKAGGLSTIEEKSLGCMYKAGNKPFKGALEYADTVPTDKKGL
                     YFMDTPGQDIDSITGMVAGGAQIVIFSTGRGTPTGSPISPVIKITGNSDTYNKMPDNI
                     DINAGRIITDGAKIADIGQEVFNEIVEVCNGKHTKAESLGHREFGIYRISSTF"
     gene            complement(9871..10146)
                     /locus_tag="L3M60_001930"
     CDS             complement(9871..10146)
                     /locus_tag="L3M60_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HBN6226596.1"
                     /translation="MNIIIHANEKDNLVTCVRPLVKGEKISIDGKSYIVNDNIPVFHK
                     MATENVKKGDVVYKYGEVIGIATVDIKAGDYVHVHNIESTRGRGDKK"
     gene            complement(10167..11141)
                     /locus_tag="L3M60_001931"
     CDS             complement(10167..11141)
                     /locus_tag="L3M60_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate permease"
                     /protein_id="HBN6226597.1"
                     /translation="MHILKSVKKIPGGLMVCPLLLGALFNTVYPQALEIGGFTTSLFK
                     TGAMSILALFCLCNGAQINIRQAGNPLTKGIVLTATKFIIGAVLGIIVSKFIGPKGII
                     GITPLAIVATMTNSNGGLFAALAGEYGDSTDVGAISILSLNDGPFFTMLAFGVTGLAN
                     VPIVALFAALIPILLGFILGNLDEDIREFLAPGTTLLIPFFAFPLGAALNFNQIITAG
                     LPGVILGLGVTIITGMGGYFVMKLIRAEHPAVGAATGTTAGNAAGTPEALAAIDPTLA
                     TIAAVSTVQVAAAIIVTAICCPMLVSYLDKREKKKKAIFKTNKNIEVN"
     gene            complement(11411..13324)
                     /locus_tag="L3M60_001932"
     CDS             complement(11411..13324)
                     /locus_tag="L3M60_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6226598.1"
                     /translation="MKSGGIIMKEILVIAPTKGTYEKSIHIVRKNKYTNIDVMFGNLK
                     EGIPLAEKSINHGTRIIISRGGTYNMLKATYNIPIVEIKVDAYDIIKSYKEVKNSDEP
                     FGIIGFNNVIYGFDIIEEILNKKITMIEIEKEEEIYDAIEKYRKKGINTYIGDTTVAH
                     IVKRLNCKGILIESREENILRAIQQAEQILEATKDEQKRRLQIEAMTDFVHDGIITVD
                     KNFKITLFNKSAEKIFGIKKENALYHNVIDVIPNTVLPQVVETMEPQIGEIQNIGSTK
                     IITNRVPIIVDGQIQGAVSTFQDSKEISSFEHTIRRSLLNKGLSAKYTFDDIIHASEK
                     TKDCIELAKKYACYDTPMIITGESGVGKELFCQSIHNYSKRKNAPFVAVNCAAIPPSL
                     IESEFFGYEEGSFTGARKKGKAGVFELAHEGTIFLDEISEIPLELQGRLLRVLQEKQV
                     MRIGSNKVIPIDVKIICASNKDLKNLMRNGLFRQDLYFRINILTLYLPSLKERKEDII
                     KLSEFFIHKYSNKYNKTPLIITPNIKKALLQREYEGNIRELEGIMERAVIVESFDSIL
                     LEKDSNEYTNNIVDKNNTDFEYNNLDLKTLEKKYIYKIYNENNRNTTKTCEILKITRS
                     TLWRKLKEIENDV"
     gene            complement(13500..14714)
                     /locus_tag="L3M60_001933"
     CDS             complement(13500..14714)
                     /locus_tag="L3M60_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBN6226599.1"
                     /translation="MNQEYVKGKPIFTNINKIPRQFPYLTDDIDTDVIIVGGGVTGCI
                     CAYYLAKNNIKSVILEKGRIAHGSTSVTTSLLQYELDDNLIDLTEVMTLKDALKAYNL
                     CASALEELDTFIELYGNKCDYARRDTLLYTANKLEIKAIKEEYNLRKENGFDVEYIDE
                     STNPFSFDLKSGLIAKNGGRELDPYKYSHHLIDVSLKNGLQVYENTEVKKVDLSNDKV
                     TAEVSYGYKVHGKKLIVATGYNTSLFTKRNFATKSNTFNIATKPLKNIASWKNNILIR
                     DNCDPYNYLRTTKDNRLIIGGEDVSFDDIEDETLANEKYDILEQRLKSMFKDIKDIEV
                     EYKYCGCFASTLDNLGFIGPDNKNKNLWYCLGYGANGILFAILGGIMLSELYLGKQNK
                     NMKLFKVDRFDK"
     gene            14958..16340
                     /locus_tag="L3M60_001934"
     CDS             14958..16340
                     /locus_tag="L3M60_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011967718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBN6226600.1"
                     /translation="MLSKDIASKVLEKCLITGGDFAEIFEEDTINNSISLIDNKVENA
                     IGGRNYGIGIRIFKGLKSIYAYTSDNSLNSLLDVAYKAAVALGKLEDGKSVILNDSIK
                     INNIHNIKIYPNSIGNRDKVNIMKVAYKSAKEFSSDISQVSVSYLDKDQKILIANTEG
                     IYVEDRRIRTRLGISSIASKGNENQTGFEGPGGCKGFEIFEEIDPEYYAKESARVAHT
                     MLHAKNCPAGNMMVAIDNGFGGVIFHEACGHSLEATSVAKGNSVFADKLGQQIASTKI
                     TAIDDGTIPNYWGSMNIDDEGNPAQKNILIENGILKSYMIDKLNGRRMGMNPTGSSRR
                     QNYKFAPTSRMTNTYIAAGEDKPEDIIKSIPDGLYAKKMGGGSVNPVTGEFNFAVSEG
                     YLIKNGEIQEPVRGASLIGKGSDVLMNIDMVGNNLKQAQGMCGSSSGSIPTNVGQPMI
                     RVKEITVGGR"
     gene            16352..17701
                     /locus_tag="L3M60_001935"
     CDS             16352..17701
                     /locus_tag="L3M60_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HBN6226601.1"
                     /translation="MELKSFKDILFKKALSEGFEECEIYYYTGENLSINIYEGEVEKY
                     NLDKSFGLSFRGKVNGKIGYSYTEILDDKAVDMLIKNVKDGVNTIENEDVQFIYEGDK
                     QYNAVKTYSKELENLEADKLIDLALEMERETKAYSDKVVNLSKCTISYSVSSNGISNT
                     KGLNLSNKTNMFIGFVVPIIEDNEQKYDGIGYNIASSIEEIKPCEIAKLGVDNALSKV
                     GGKSIPSGKYKTILFNEAMVSLLSTFSGIFNADSAQKGLSLLKNREGDMIASPIVTIV
                     DDPLLENGMASTPFDDEGVATFKKEVVLNGKLITLLHNLKTANKAGVKTTGNGIKSSY
                     SSPISIYPTNFYIEKGDKSLDEIMQDIGEGLMVTSFAGLHSGANSVTGDFSLAAKGFY
                     IKEGKKVFPVEQITVAGNYFDLLKDIEVIGEDLEFPMSSIGSPSVVIKELSVAGKDE"
     gene            17851..18384
                     /locus_tag="L3M60_001936"
     CDS             17851..18384
                     /locus_tag="L3M60_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBN6226602.1"
                     /translation="MSDAHYNMHKRNGYSSDEPYPEIKVLGPNKYYAELLMDDYAGVS
                     SEFTSVNQYLYHNFDLDEKHRELNEMWVNISITEMLHMEILAKTIRLLGGNPVYRGST
                     SSCGAYWNGGFVCYGDSICNRLKLDLHLEHVAINNYYKDISLIEDPYIKAILNRIILD
                     EKLHVSLFEKAIEKYCK"
     gene            18468..19277
                     /locus_tag="L3M60_001937"
     CDS             18468..19277
                     /locus_tag="L3M60_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6226603.1"
                     /translation="MYYVYVNDINIAVYDLNPSAKKTVLFIHGWPLGHKIFEYQTNIL
                     PKLGYRTVSIDLRGFGKSDATSGGYTYSQLADDIYKVVHAIGLKDFTLVGFSMGGAIV
                     LKYMSLFNGYGVSKLVLAAAAAPSFVQRPPEFPYGMTREDVNKLIAQACTDRPEMVTD
                     FGEKVFASNPPESFRRWFNDIGFSASGIGTIGTAVSLRDEELFDDLKCVRVPTGIFHG
                     KLDEICPYEFAVFMNEKIEDSILYTFDYSGHAIFYDELKLFNQEFLQLLEE"
BASE COUNT         6723 a         2606 c         3080 g         6997 t
ORIGIN      
        1 tttaaaattt cgatttttca taattgtaag gtatactagt gccaacaact acatcttcaa
       61 ttttttcaat aataagccaa tcatccatat ttccttgatg gtcaaaatca agaggtgtta
      121 tttcttcaac attttcaaat tcaactaagc ataaacattt tttatgccaa cgaacttttt
      181 gcttttcgga taaatttaat tttggttgat tttcatttaa aattttagaa atctcgtctt
      241 ctgttaactt aacatagttt tctacttttt taacaatggc ttttgcagat atttttgcag
      301 ttcctttttc cataaaataa agtaactcat ccttaaatac tctactgtgt ggtatttttc
      361 taccagcagc acctctaatc accattgttt ttgttccagc taagatttta tctaaaacct
      421 tttctccttt ttttcctgca ttatcacaat atactaaatg taccattttt cgaatcctcc
      481 ttactaactt ccttatctat aagttgcatt ttatcattgt tcaactaaaa agtcattcta
      541 tttatatcta attttttaaa aaatactatt taatatgaga aaaccttatc tgagctatat
      601 ataattaaga ttaattagga atttttatct ttttacaaaa taatattgac taaaaataga
      661 aaaatgctat aataaatata tgcttatgat aatcaatatc atataagtat aataaacagc
      721 taggagaaat aatcatgaaa aaaataaaat cacttgcaat atttatatct attataacta
      781 cgttagtact tgttacagca tgttcagata agaatgcaga ggataaagat aaatcagaga
      841 caagagttgt acaatctgtt aaaggagagg ttaagatacc atcaaatcct aaaaaaatag
      901 ttgacatatc aggaagtagt gaagaactac tattagcagg ttataaacct attgcaacag
      961 caaatgtaga ctcatatgaa actgataaat taccaagtta cataagagaa gaacttaaag
     1021 atgtaaagat agttgggcac tcaatgatgg atactatgga tatggaagct atattagaag
     1081 taaatcctga tttaataata atgagtcaaa gacaagaaaa aatatatgac caattaaaag
     1141 aaatagctcc tgtagttatg atgaaagatt atgcaaatga ctggagaagt aagttaactg
     1201 atgtttcaag gttatttgac aaagaagaag aaactaaaag ttggctacag aagtatgatg
     1261 aaaaagctac aaagttaggt aaagaagtta tagaaaagaa tggagaaaaa acttatttac
     1321 cagtgttggc aagttcaggg caatttatgg tatttagtga tggtggaata ggtactctaa
     1381 tcaatgatga catgaaatta gctagaccta agaatatgcc aaaacaggat ggaataacac
     1441 taccaatgat aagtatggaa ggtttaacag atatagatgc agaccatata gttgtaattg
     1501 ctacagaatc agataaaaaa gatttagaaa atagtgctat ctgggctcaa ataagagcag
     1561 taaaagatgg taatgtaaca atacttgatt ctgctccatt tttcagtcaa tcatacaatc
     1621 caatgggaaa agagttatta ttagaatcag ttaaaaatga attaacaaag taattcttaa
     1681 cttagtaggt gtgagataat gatattcatt tttcacacct atttttaata aagcttaaaa
     1741 taaaaaatat ttaatagatg atattatact gactattaaa agggaaaagg aggtaggata
     1801 attgactaag gtaagcaaaa aaaatataac ttatttattg atattagtaa gtatagttgt
     1861 acttgtattt ggaattttat tatccataac tattggggca aaagatatga atctatcaac
     1921 tgttatagat agcttgataa aaatggaaga tggaataaat attagaatag taagggatgt
     1981 tagacttcca agagcaatag cagctacact tgttggaggt tttttagctg tttccggggc
     2041 aataatgcaa gggataacaa gaaatcctat agctgaacct tctgttatag gaattacaca
     2101 aggagctaca tttacaattt caatatcact ggtacttcaa aaaaaacttc ctcaacttat
     2161 acatggaagt tttagtgtaa tgatgtttgc atttattgga gcaagtataa gtggattatt
     2221 tatatatttt ataagttcta aatcaagagg aagagttaat aatgtaaaat tagcacttgc
     2281 aggagtagct cttggtacat tgttaatttc cttggcatca gcaatatcta tgtattttaa
     2341 cctatctcaa cagttgagtt tctggatatc aggagggctt gtaggagtga attgggaagg
     2401 tataaaattg ctatttatag ctggaggtat tggatttatt ttagctatta ttatggctcc
     2461 aagaataact atattaagtc ttggagaaga agtggcaata ggattagggc aaaaaactaa
     2521 catagttaga tttgtatgta tagtacttgt gatactaatg actggagcat ctgtttcagt
     2581 gtctggcaat attattttta taggtcttat agttcctcaa atagctaaag gaatagtagg
     2641 ttcagattat aagtatataa taccgagttc tttagtatta ggagctgtat tgcttgtata
     2701 tacagatata ttaagtagga tgataaatcc tccatatgag acaccagtag gttctataac
     2761 agcacttata ggagtgccaa tatttatata ccttgttaga aagggggaga aataggtact
     2821 atgaaaaata ttataaaaat aaattctaaa acattgcttg ttatattaat tttagtggta
     2881 cttattttta tcactcttct agtgagttta aatatgggct cattttctat agaaccaatg
     2941 gatgttataa agactttttt aggtcagggt cagcgtaatc atgaaattgc gatatttaaa
     3001 ttgagattac caagaatagt aatagcactt ttggttggtt ctgcattatc aacagcaggt
     3061 gtaatacttc aaggagttac aaagaatgat ttagctgatt ctggtatttt gggaataaat
     3121 tcaggtgctg cattgtttgt agtagtttat atatacctta tgaatggtaa tgtatatgaa
     3181 ggtatgagta atctgactgt atttacaatg cctattgttg cacttgtagg agctttattt
     3241 ggagcatttc ttatatattc tcttgcatgg aaaggtggta taaattcgtc aagattacta
     3301 cttattggta taggtgtaaa tatagctttt acttcaattt tgactatatt tcaattgaaa
     3361 tttacaacac aagaatttaa tagggttatg gcgtggacat caggaagtgt ttggggaact
     3421 agttggaaat atgtaatagc tgtacttcca tttatagtga tttttatggg gataactatt
     3481 tataaatcca aatatataga tgtattaaac ttgggtgatg aagtatctat aagtcttggt
     3541 gtagatgtag aaaaacaaag aagaagactt ataatatatg cagtaatttt atctggtgta
     3601 tcgacagcag tagcaggtag tatttcattt ttaggtttaa tagctccaca tataggtaga
     3661 aaaataatag gtccaaagca taaaagatta ataccagtat cagcattgat aggaacatta
     3721 ttgcttttaa tagcagatac tatatcaaga aatcttctag cacctataga aatacccgta
     3781 ggtatagtag tttctataat aggagtgccg tactttattt atttaatgtt ggtaaatgat
     3841 taggaaaggg tgttgttatg aactgtataa atactaagaa tctaaatgta tcatatggaa
     3901 atacagatat tataaaaaat ctaaatttaa atataccaaa aggaaaaata actactatca
     3961 taggggctaa tggttgtgga aaatcgacaa tacttaaaac tataggtagg attattagcc
     4021 ctaagagtgg aaaaatatat ataaatggag aggatatcta caaaaagaag tctaaagaaa
     4081 tagcaaagga tatggctgta cttccacaaa gtccacaagc tcctagtggt cttactgttt
     4141 cagaattaat ttcttatgga aggtttcctc ataaaagtgg atttggtaat tctagtaaag
     4201 aagacagaga gattgtaaat tggagtttag aagttactgg aattgcagaa tttaaggata
     4261 gaaatatgga tgatttatca ggagggcaaa ggcaaagggc atggatagct atggctttgg
     4321 cacaagagac tgatatatta ttattagatg agcctacaac atatttagat ttggcacatc
     4381 aattagaaat actaaaatta ttggatgaat taaataaaaa acaaggaaga actattgtca
     4441 tggtaataca tgaacttaat aatgcagcaa gatttgctga ccacatgata ggtattaaga
     4501 aaggaaaaat agtttgtgaa ggtgatgcat atgatgttat gacgaaagat aatctaaaag
     4561 aattatttaa tattgatgca gaaatagcaa cagacccaag aacaaataaa ccaatttgta
     4621 taacttatga catagtgtaa atggtgacaa catggagaat caagaggcat taagacatga
     4681 aaaaatatgg attttattat ttaaatattc gatacctgca ataatagcaa tgatggtaac
     4741 ttcactttat aatgtagtag atagagcttt tattggttct atggagggca ttggttctat
     4801 agctattgca ggtcttggtg taactatgcc agtatttact ctaataatag cttttggaat
     4861 gttggtatca gtaggtgcta gcacaagatt atctataaag ctaggtgaaa gaaatagaga
     4921 agaagcagag aagatattag gtaatgcttt gacattatct ataattatct ctttaattat
     4981 aactatatta ggattaattt ttttagaaga tatgcttttt attttgggtg ctagtaaaga
     5041 ctctatttct tatgcaaaag actatatgag tgtcatatta gttggaagta tattcaattt
     5101 agttgcattt tctcttaata atgcaattag ggcagaaggt aatccaaaat tagcagcaag
     5161 gacaatgatt gtagggtgtg tattaaactt gatattggac cctatattca tatttgtgtt
     5221 taatttaggt ataaaaggag cagctatagc aacagtactc tgtcaaatag tagtattctt
     5281 ttgggtaaca cattatttta tgagaggtaa gtccaattta aaactaaaaa tatgtaattt
     5341 aaaattgaat aaagatatag taaagaaggt atttgcaata ggtatgacac cttttgctat
     5401 ggaggtagct ataagtataa ctcacatgtt tacaaataat tcgctaaagg tttatggtgg
     5461 ggatttagct attggggcta tgacagcact tacatctatt ttattgatgt ttatgatgcc
     5521 tgtatttgga ttgaatcaag gtatgcaaac tataatatca tacaattatg gtgcaaaaaa
     5581 atatgagcgt gcaaagaaaa ctttgatatt atctatcata gtgtcagttg tcatattatc
     5641 gtttgggttt ttagtagtac aagttttccc agaagtattt gtaggtatat ttaataaaga
     5701 cccagattta atgaatatag cagtaagagg tataaatata aatctaatta cattgcctat
     5761 aatgggaatt tctattgtag ggcctgtgta ttttcaatgt ataagtaaag ttaaacactc
     5821 aatgttttta actcttttaa gacaatttat tttatttata ccattaatca ttgtacttcc
     5881 aattcaattt aatttagatg gagtttggtt ggcacagcca attgcagatt ttattgctat
     5941 gataattgta ttattattct taaaaaggga gtttaaaaca agtcaagtat aagaaatcta
     6001 gaaattaaaa tgaaattaga agataaaaag acttgtcttt ataaagtgat taaaattatt
     6061 ttataaagac aagtctttta ttttatttaa cgaaactatc catactggtt tcttctagtg
     6121 tttttccaaa agtcattttc tcaaatattt cttgaggcag aggttttgaa aaaacatatc
     6181 cttgtatcat atcacaatta atttttttaa gataatccat ttgcaagatt gtttcaacgc
     6241 cttctgagat actattcata tttaattttt ttgtcagatt aactatagat tctattatat
     6301 gattttcagc aatattatct gcattaggtg aactaaaaaa agcacggtca agttttaatg
     6361 tatcaacttt tatttctttt aacatattga gtgaagaata tccacttcca aagtcatcta
     6421 gtgaacattg aaaaccttct tcatggattt ggtctattat attaattagt ttttgtagat
     6481 tttcaaatac aagtgtttca gttaattcaa tttctaagag cttagctgga atatcatatt
     6541 tattacgaat cattctgtat ttttctaaaa aatctatatt atttaaatta gcacgtgaaa
     6601 aattaactga aacaggtatt ggagtttctc cattatctat ccatttcctt atcatactac
     6661 atactttttc aaaaacatag tggtctagtt ttgtaataaa accatttttt tcaaagaaag
     6721 gaataaactc atctggtggt ataagacctt tatttggctc ttgccatctt actaatgctt
     6781 cagcacctac aatagtatta tctttaagac tgattttagg ctgaagatat actataaatt
     6841 cgttattatt taatgcttcc tccattctat tttccatttc tttttcttta atcattttta
     6901 agcgttctaa atcactataa aaaacacatg tataaagtgg atgatttcca atatttcctt
     6961 tattgttttt tcgtgcaaca tttgcacggt cttgaattga aactaaaggt agatttgggt
     7021 catcaatttt atatacacct atagatagag ctataaaata cttgcattct tcatttttat
     7081 tgaaactgtt aatatcatta gtaatgtttt ctatgtaact aagaatttct ttttgaggaa
     7141 tagttttaat aagtacatta aactggtcat tagaaactcg acaaagtaat tcttctggtt
     7201 ttagataatt actaataaca tcatgtatgt actttaatgt tttatcacct gcttcactac
     7261 caaaagaatc attaataagt ttgaacttct gaatgtttat agataccaat gaatagcttt
     7321 ctgatgaaga tgattgtata atttttttag cttccatatc aaagcgtgct ttgctgtaac
     7381 ctttagttac agagtctaca tatgcgattt ttataagttg tgccctgttt ttttgattta
     7441 taaacataat caatataata agcaataaaa atagtataac aattaaaata ttgattaaga
     7501 ttgctttttt tagaagtaaa tcaaactgtg cacttgctgt ttttgttgga acaatcgaaa
     7561 ggagatataa gttttcttct tgtaatggaa tatagtgcat tgctttagaa ataccatcat
     7621 ctaatgtata atacatagaa ccagttttgt tcttttttaa gtttgttttc atttcatcaa
     7681 gcttattttt atcaacctgt tttcctttat gttcaatcat gtcaaaaaaa tttaccacat
     7741 tctttggagc atttttgttt gccgaactta caataaaatt tccattttgg tcgataattt
     7801 gggaaaatcc ttcaccatta aagctttcta cacttaataa atttctcata gtatcaatag
     7861 tattccaagc agcaacagct ccaacaattt tatcatcttt gtatagtggt acagaatata
     7921 aaattccata tgtaccaaat ggtgatttaa caggcgtaat tgtttcttta ccttgaaggg
     7981 cttttatata aaagtcttgt cctttaacat tacctgtatc tttaagggca cttatgaaat
     8041 ttccattaga attgataata cctaaccaat caaattcaaa taattttaat tttttggata
     8101 agtatttatt tacttctttg tcactatcat taacctcaaa ataagtatct cccacagaat
     8161 atagtgtctt aaatatactt ttacatttca catcaattgt ggtagaaatg ctttgtgaaa
     8221 tttctataag atatcgttct gtttcattat gtgttacttt atttagattc ataataaaag
     8281 tgacacatgt taaggacaca attataagga gtgtcaatag taaagtagct attatttttt
     8341 tattttttgt gttctcaaag ttcattcttt tgtttcatcc tccatcctct atttgtttac
     8401 aaataagaat tctctcagac atattatacc aaatatcttt tcatacaaag acattaaatt
     8461 ttatttattt gactttattt aatcttttta gggagtagat aagatatatt tatagtttaa
     8521 atcgtatttt atgatgtttt aagtaaaaga ggataaaaag gaactgttct caaaataact
     8581 ttgatacagt tcctttttag tattgtaaat ttttataaga ttagaaaggg tttttagttt
     8641 atacatctac ataagtatta tctttataaa aaatatttta ttgtctattg caatctaaaa
     8701 tgtagatgaa attctgtaaa taccaaattc tctatgacca agactttcag ctttggtatg
     8761 cttaccatta cagacttcca ctatttcatt aaatacttct tgacctatat ctgctatttt
     8821 agcgccatct gttataattc tacctgcatt tatatcgata ttgtctggca ttttgttgta
     8881 tgtatcacta tttccagtga ttttaataac tggtgaaata ggacttcctg ttggagtacc
     8941 tctaccagtt gaaaagatta caatttgtgc accacctgct accataccag tgatagaatc
     9001 tatatcctga cctggagtat ccataaaata taaacctttt ttatcagtag gtacagtatc
     9061 agcatactca agagcacctt tgaatggctt gttgcctgct ttatacatac atccaagtga
     9121 tttttcttca attgttgaaa gtccgccagc tttattgcct ggagtaggtt gaccactgcg
     9181 taaatcttct cccatagtaa ttgctctttt ttctacatca cttacaaatt taagaaactt
     9241 atccttcatt tttccatctt caaaacgagt agccaacaaa tgttctgcac caattacttc
     9301 tgtagtttca cttagtatgc tacttccacc ttcatctata agaagatttg atgcaacacc
     9361 tattgaaggg tttgaagcta ttccacttgt tgggtcagaa ccaccacatt caagtgctaa
     9421 tataatttca ctcatatcaa attcaactct tacttctttt gatacttcac gagccatttt
     9481 acctactatt tcagctccct tagaaactgt ttttaatgtc ccaccacatt cttgtataac
     9541 aacataatct accttttttc ctgttgtagc tatttcagat gataagtctt ttgcttgaat
     9601 accatcacat ccaagaccaa ctactaaaac tgcggcgaca ttaggatttt ttccaaatcc
     9661 tattaatgtc tttgctgtaa gctctatatc agaaccaatt tgacaacatc catgttgatg
     9721 agggatggct attgctccag gtacaagtga tgctattctt gtagctgtct cacttgaaca
     9781 tacagatgtt ggaataacta aaacatgatt acgaatacca aattgtccat tttctcttaa
     9841 ataacccatt aatttcattt taacttcctc ctatttttta tctccacgac cacgtgtact
     9901 ttcaatgttg tgtacatgaa catagtcacc agcttttatg tctactgttg caattcctat
     9961 aacttcaccg tatttgtaaa caacgtctcc tttttttaca ttttctgtag ccatcttatg
    10021 aaagacaggt atattatcat ttactatgta agactttcca tcaattgaaa ttttttcacc
    10081 tttaactaaa ggtctgacac atgtaactag attatctttt tcatttgcat gaataattat
    10141 attcattaat tttacctcct taagtattaa tttacttcaa tatttttatt tgttttaaaa
    10201 atagcttttt tctttttttc tcttttatct agataggaaa ctaacattgg acaacatatt
    10261 gctgtaacaa taatagcagc agcaacctgt actgttgaaa cagctgcaat tgtagctaga
    10321 gtagggtcga tagctgctaa agcttcagga gtaccagctg catttccggc tgttgtacct
    10381 gtagctgcac ctactgctgg gtgttctgca cgtataagtt tcattacaaa atacccaccc
    10441 atacctgtta ttattgtaac tcctaaacca agtataactc caggtaatcc agcagttata
    10501 atttgattaa agtttaatgc tgcccctaaa ggaaaagcga agaaaggaat taataatgtt
    10561 gtaccaggtg caagaaattc tcgtatatct tcatcgagat taccaagtat aaatccaagt
    10621 agtattggta taagtgctgc aaatagtgct actataggta catttgcaag tccagttact
    10681 ccaaaagcaa gcattgtgaa aaaaggacca tcatttagag ataagataga tattgctcca
    10741 acatctgtag agtctccata ttcacctgca agagcagcaa atagaccacc atttgaatta
    10801 gtcatagtag caactatggc aagaggtgta ataccaatta tacctttagg tccaataaat
    10861 ttactaacaa taatacctaa tactgcacca attataaatt ttgttgctgt aagtactata
    10921 cctttagtca atggattacc agcttgtctg atgttgattt gagctccatt acatagacaa
    10981 aatagagcaa gtatgctcat tgcaccagtt ttaaataagg atgttgtgaa tcctccaatt
    11041 tcaagagctt gtggatatac tgtattaaag agtgctccca ataacagggg acagaccata
    11101 agtcctcctg gtatcttttt cactgatttt aagatgtgca tctagtatca ctccctttca
    11161 tttttatata ggtatttaaa agcaattact atgccaatat atagagttta tatttttagt
    11221 tattatgatt atttttgata atcgattgaa atactgtaga ttacttataa ttaaaagtat
    11281 gattgaggag aattttacat taaaaagatt ctttttatca tataattgta ttttttatta
    11341 aaaataaata aacgtcttat gtattaaaat taaacataaa acgttttgat atattaagta
    11401 tgtattattt ttaaacatca ttttctattt cttttaactt acgccataga gtagagcgag
    11461 ttatctttag aatttcacag gtttttgtag tatttctatt attttcgtta taaattttat
    11521 agatatattt tttttctaaa gtttttaaat ctaaattatt atattcaaaa tcagtattat
    11581 ttttatcaac aatattattt gtatattcat tactatcctt ttctaataaa atcgagtcaa
    11641 atgattccac aataacagcc ctttccatta taccttccaa ttctcttata ttaccttcat
    11701 attcccgttg aagaagtgct tttttaatat ttggagtgat aattaaaggc gtcttattgt
    11761 atttgtttga atatttatgt ataaaaaatt ctgataattt tataatatct tcttttcttt
    11821 cttttaatga aggaagatat aatgttagta tatttattct aaaataaagg tcttgcctaa
    11881 ataaaccgtt gcgcatcaga ttttttaagt ctttatttga agcacagata atttttacat
    11941 ctataggaat aactttatta ctgccaattc tcataacttg tttttcctgc aataccctca
    12001 ataatcgacc ttgaagttca agagggattt cacttatttc atctaagaag attgtacctt
    12061 catgtgctag ttcaaatacg cctgctttgc ctttttttct agcacctgta aatgaacctt
    12121 cttcataacc aaaaaactca ctctctatta aagatggagg tatggcagca caatttacag
    12181 caacaaaagg tgcattttta cgcttactat agttatgtat gctttgacaa aatagttcct
    12241 taccaacgcc agattcacca gtaataatca ttggtgtatc ataacaagca tatttttttg
    12301 cgagttctat acaatctttt gtcttttcac tagcatgtat tatatcatca aaagtgtatt
    12361 tagcagagag acctttattt aataagctcc ttcttattgt atgttcaaaa gagcttatct
    12421 ccttgctgtc ttgaaatgtt gaaacagctc cttgaatttg accatcaaca atgattggaa
    12481 ctctatttgt aataattttt gtacttccta tattttgtat ttcaccaatt tgaggttcca
    12541 tagtttcaac cacttgtgga agaactgtgt ttggaataac gtctataaca ttatgataaa
    12601 gtgcattttc tttttttata ccaaatattt tttcggcact cttattaaat aatgttattt
    12661 taaaattttt atcaactgtt attattccat catgtacaaa atctgtcata gcttctatct
    12721 gtaatcttct tttttgttca tccttggttg cttcgagtat ttgctctgct tgttgaatgg
    12781 ctctaagtat gttttcttct ctagattcga ttaatattcc cttgcaatta agtctcttaa
    12841 caatatgtgc aacagtggtg tctcctatat acgtgtttat acctttcttt ctgtactttt
    12901 ctatagcatc ataaatttcc tcttcttttt caatttcaat catagttatt ttcttattca
    12961 atatttcttc tataatatcg aaaccgtaga tgacattatt aaatccaatt attccaaatg
    13021 gttcatctga attttttacc tctttgtagc tttttataat atcataagcg tctactttaa
    13081 tttcaactat aggtatattg taagtggctt ttaacatatt gtaagttcca cctcggctta
    13141 tgataattct agtaccatga ttaatacttt tttcagcaag aggtatacct tcttttagat
    13201 tgccaaacat aacatcaata tttgtatatt tgttttttct aactatatga attgattttt
    13261 catatgttcc tttagtggga gcaataacga gtatttcttt cataataatc cctccagatt
    13321 tcatcaaata aacttaaata ttatcttatg actaaatttt attagttata ttttaatatt
    13381 ttactttgta tttaattata aataaagtat tttaaaaaga acattataaa agactgtatc
    13441 aaagttaact tttagaatta taaagtttca tctttttaca gtcttttaat ttacaatatc
    13501 tatttatcaa atctatctac tttaaatagt ttcatatttt tattttgttt gccaagatat
    13561 aattcactta acattatacc tcctaaaatt gcaaagagta tgccatttgc tccataacct
    13621 aagcaatacc atagattttt atttttatta tctggaccaa taaaacctaa attgtctaaa
    13681 gttgaagcaa aacaaccaca atatttatat tcaacttcaa tgtcttttat atccttaaac
    13741 atacttttca atctttgttc taaaatatca tacttttcat tagctaaagt ttcatcttct
    13801 atatcatcaa aacttacatc ttcaccacct ataattaatc ggttgtcttt agtagttctt
    13861 agataattat atgggtcaca gttatctcta attaaaatgt tatttttcca agaagcaatg
    13921 tttttaagtg gttttgtggc tatattaaaa gtatttgatt tagttgcaaa atttcttttt
    13981 gtaaataaac ttgtattgta ccctgtagct acaattaatt ttttaccatg aaccttgtat
    14041 ccatacgata cttcagcagt aaccttatca tttgataaat ctactttttt tacttctgta
    14101 ttttcatata cttgaagacc attttttaaa cttacatcaa tcaaatggtg actatattta
    14161 tatgggtcta attctcgtcc tccattcttt gctatcaatc ctgattttaa atcaaagcta
    14221 aaagggtttg tagattcatc aatatattca acatcaaatc cattttcttt tcttaagtta
    14281 tattcttctt tgatagcctt aatttcaagt ttattggcag tataaagtag agtatctctt
    14341 ctggcataat cacatttatt tccatatagt tctataaatg tatctaattc ttcaagtgct
    14401 gaagcacaga gattgtatgc ttttagagca tcttttagag tcataacctc tgttaaatct
    14461 atcaaattgt catctaattc atactgtaac agagaagttg ttacgctagt gcttccatgt
    14521 gcaatccttc ctttttcaag aataacagac tttatattgt ttttagctag atagtaagca
    14581 caaatacatc cagttacgcc accaccaaca attataacat cagtatcaat atcatcagta
    14641 aggtatggaa attgtcttgg aattttatta atattagtaa aaataggctt tccttttaca
    14701 tattcttgat tcatatattt cactcctaaa atttattatg tatgagtgta ttattttcta
    14761 tagcaaataa tattatacat tttaattatc tagttatatg ggtatgtata gataaaatgt
    14821 gataacattt tagcataaaa catatatata atatattcat aatcacctta ttttaagtaa
    14881 tatagtatat aatgtttgtt aacaagtaaa aagattgaat aaaagttaat taaaaattta
    14941 aagtaggagg taaatttatg ctttcaaaag atatagcttc aaaggtatta gaaaagtgtc
    15001 taattactgg aggagatttt gctgagattt ttgaagaaga cactataaat aattctatta
    15061 gtttaataga taataaagta gaaaatgcaa taggtggaag aaattatggt ataggaataa
    15121 gaatatttaa aggattaaag agtatatatg cttatacaag tgacaattca ttaaatagtc
    15181 tgttggatgt agcatacaaa gctgctgttg ctttaggtaa attggaagat ggcaaaagtg
    15241 taatattaaa tgacagtatt aaaataaata atatacataa tataaaaatt tatccaaatt
    15301 caataggaaa tagagataaa gtaaatatta tgaaggttgc atataagagt gcaaaagaat
    15361 ttagtagtga tatatctcaa gtaagtgtta gctatttgga taaagaccaa aaaatattaa
    15421 ttgcaaatac tgaaggtata tatgttgaag atagaagaat aagaaccaga ctgggaataa
    15481 gctcaattgc atctaagggt aatgaaaatc aaactggatt tgaaggacct ggaggttgta
    15541 aagggtttga aatatttgaa gaaatagatc cagaatacta tgcaaaagag tcagctagag
    15601 tagcacatac tatgttgcat gctaaaaatt gtccagcagg taatatgatg gtagcaatag
    15661 ataatggatt tggtggagta atattccatg aagcttgtgg acattctttg gaagctacat
    15721 cagttgcaaa aggtaactct gtatttgcag ataaattagg tcaacagatt gcctctacaa
    15781 agattacagc tatagatgat ggtacaatac caaactactg gggctctatg aatatagatg
    15841 atgagggaaa tccagctcaa aaaaatatac tgattgaaaa tggaattttg aagtcttata
    15901 tgatagataa acttaatggt agacgaatgg gaatgaatcc tacaggaagt tctcgtagac
    15961 aaaactacaa atttgctcca acttcaagga tgactaacac ttatattgct gctggagaag
    16021 ataaaccaga agatattata aagtcaatac cagatggact ttatgctaaa aaaatgggtg
    16081 gtggttctgt aaatcctgta acaggagagt ttaattttgc agtttcagaa ggttacttaa
    16141 taaaaaatgg agaaatacaa gagccagtta gaggtgctag tttaattgga aaaggtagtg
    16201 atgtacttat gaatatagat atggttggga ataacttaaa acaagctcag gggatgtgtg
    16261 gctcatcatc aggaagtata ccaactaatg taggtcaacc tatgataagg gtaaaagaaa
    16321 ttactgtagg tggaagatag gggggataat tgtggaactt aaaagtttta aggatatatt
    16381 atttaaaaag gctctaagtg aaggttttga agagtgtgaa atatattatt atacaggaga
    16441 aaatttaagt ataaatatat atgaaggaga agttgagaaa tacaatcttg ataaatcttt
    16501 tggtttatct tttagaggga aggtaaatgg aaaaataggt tattcttata cagaaatttt
    16561 agatgataaa gctgttgata tgttgataaa aaatgtaaaa gacggagtga acaccattga
    16621 aaatgaagat gtacaattta tatatgaagg tgataaacaa tataatgctg taaagactta
    16681 ttctaaagaa ttagaaaatt tagaggcaga caagctgatt gatttagctc tagaaatgga
    16741 aagagagact aaggcttatt cagataaggt ggtaaattta tctaaatgta ctatatctta
    16801 ttctgtatca agtaatggta tatctaatac taaaggtctt aatttaagta ataaaactaa
    16861 tatgtttata ggatttgtag ttcctattat agaagataat gagcaaaaat atgatggaat
    16921 aggatataat atagcaagtt ctatcgaaga aataaaacct tgtgagatag caaaacttgg
    16981 tgttgataat gctttatcta aagtaggtgg aaagagtatt ccatcaggca aatataaaac
    17041 tatattgttt aatgaagcta tggtatcttt gcttagcaca ttctctggaa tatttaatgc
    17101 agattcagct caaaaaggat tatctttatt aaaaaataga gaaggagata tgatagcatc
    17161 tcctatagtt acaatagttg atgacccact tttagaaaat ggtatggcat ctactccttt
    17221 tgatgatgaa ggagtagcta catttaaaaa agaagttgtt ttaaatggaa agcttataac
    17281 tttacttcat aacttaaaga cggctaataa ggcaggtgta aagactacag gaaatggaat
    17341 aaaatcttca tattcatcac ctataagcat atatccaact aatttttata tagagaaagg
    17401 agataaatct ctagatgaaa ttatgcagga tataggtgaa ggattgatgg ttactagttt
    17461 tgcaggtctt cattcaggtg caaattcagt aacaggagat ttttctttag ctgctaaagg
    17521 gttttatatt aaagaaggga aaaaagtatt tccagttgag caaattacag tagcaggaaa
    17581 ctattttgac ttgttgaaag atattgaagt tataggggaa gatttagaat ttccaatgag
    17641 tagtataggt tcaccttcag ttgttattaa agagctatca gtagctggaa aagatgaata
    17701 attaaaaagt aattttgtgg ataaaaggtc atgttaattt taattaatat ggctttttat
    17761 tatttaagta gattttatta tctaagttat atgaaaaagt cgtgatgcat acaattaata
    17821 tcatacatta aatttaaagg aggagatatt atgagtgatg cacattacaa catgcacaag
    17881 cgaaatggat attcaagtga tgagccatat ccagaaataa aagttttagg tccaaataag
    17941 tattatgcag aattattgat ggatgactat gctggagtat caagtgaatt tacgagtgta
    18001 aatcaatatc tataccataa ttttgattta gatgaaaagc atagagagtt aaatgaaatg
    18061 tgggtaaata tatctataac agaaatgtta catatggaga tattagcaaa gactataaga
    18121 ttattgggtg gaaatcctgt atatagaggt tctacaagtt cttgtggtgc atattggaat
    18181 ggtggttttg tatgctatgg agactctatt tgtaacagat taaaattaga tttgcatttg
    18241 gaacatgtgg ctattaacaa ttattataaa gatataagtc ttattgaaga cccttatata
    18301 aaagctatat taaatagaat aatcctagat gaaaaacttc atgtaagttt atttgaaaag
    18361 gcaatagaga agtattgcaa gtaaatttta aaatttagtt attcattatt ttattcatat
    18421 atataaaata actatcatac attttcttga agggggttgc tgtttatatg tattatgtat
    18481 atgtgaatga tataaatata gcagtatatg atttgaatcc gagtgctaaa aaaactgtac
    18541 tttttataca tggttggcca ttaggacata agatatttga atatcaaaca aatatattgc
    18601 caaaactcgg atatagaaca gtttcaattg atttaagagg ttttggtaaa tctgatgcaa
    18661 catcaggagg atatacttat agccagttag cagacgatat ttataaggtg gtacatgcta
    18721 ttggtttaaa agactttacc cttgttggtt tttcaatggg aggagcaatt gtacttaagt
    18781 atatgagttt atttaatgga tatggagtgt caaaattagt attagctgca gctgctgcac
    18841 catcatttgt acaaagacct ccagaatttc catatggtat gacacgtgaa gatgtcaata
    18901 aattgatagc acaagcatgt actgatagac cggaaatggt aactgatttt ggagaaaaag
    18961 tatttgcatc aaatccacca gaaagcttta gaagatggtt taacgatatt ggttttagtg
    19021 catcaggtat tggaactatt ggaactgctg tatctttgag agatgaagaa ttatttgatg
    19081 atttaaaatg tgtaagagtg cctactggta tatttcatgg gaaattagat gaaatatgtc
    19141 catatgaatt tgcagttttt atgaatgaaa aaatagaaga ttcaatttta tatacttttg
    19201 attatagtgg tcatgctata ttctatgatg agcttaaact atttaatcag gaatttttac
    19261 agcttttaga ggaatagtta tattttatta tcacttagaa aaagagtaat attatgtgaa
    19321 gtgatttaag ctaaatttta tgatgtattt tacactaaac aaatagggtt ttagaacttg
    19381 taactaagtt ctgagaccct attatt
//
LOCUS       DAFNNB010000064        19301 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0065, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000064 DAFNNB010000000
VERSION     DAFNNB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 19301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19301
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0065"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            476..988
                     /locus_tag="L3M60_001938"
     CDS             476..988
                     /locus_tag="L3M60_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226604.1"
                     /translation="MKVKKFKSGIVMALTVMVIGASLTNVSAMELNKSKEFETKYNEM
                     KRDFQEEYGKEDKFNITLDDEFTKEEVNGAKGIIKTTNKVNNEVVVLNYFDEMKSGIE
                     FELKPKAEKNVILREEFQQGYGKEDKFKVVLDDEFTKEEVNGAKGIIKTTDKETGEVI
                     VYNYFDELEK"
     gene            complement(1293..2390)
                     /locus_tag="L3M60_001939"
     CDS             complement(1293..2390)
                     /locus_tag="L3M60_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBN6226605.1"
                     /translation="MKKTYLFSPGPVMVTDKVRNSLLHYDICHRGNEFMDLFKDTQQK
                     INKLYNATSNYYSLIVSGSGTSVNECVLSSIFDKEDAVLLVSNGVFGERLEEILSTYN
                     VKTYKPNFEWAEYPDLNILEKYIIEHSDIKVIAMVFHETSSGMINPVPELGKLAKKYN
                     KIFFVDAISASAGEYIDVEEFNIDIITGVGGKALGAFPGSAYLCAKESILQDIKENQC
                     KNVYLNLYKHYKVAKSSSQTPNTPNVTLIFALNEALTEFLEDDSKIERYKECSAILRN
                     GMEELGLTFLLPDKYMSNTVTSVFLPKEINVDEFILGLERESGYVVYPGKGKFLDANI
                     FQVANMGEIYPEDCYKFLDILKSRLEIKKVI"
     gene            2683..4209
                     /gene="glpK"
                     /locus_tag="L3M60_001940"
     CDS             2683..4209
                     /gene="glpK"
                     /locus_tag="L3M60_001940"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBN6226606.1"
                     /translation="MKYVLAIDQGTTGTRAVLINEKSEFVFSDYMEHTQIYPQSGYVE
                     HDPIEIWNNTKTVSNNVLQQAFENGINESDIKGIGIDNQGETVMLWDKNTGKPLYNAI
                     VWQCRRTYDYVENLKNIPGLENKIIDKTGLIIDPYFSGTKIKWVIDNVKGVKEKIKNG
                     EVLAGTLDSWLIWKMTGGKSFLTDVSTAARTMLFNIRDMDWDKELLDIIGVPRSILAD
                     IMPTSGDFGTTDKEAFCGVSIKITASMVDQPAALFGNGCFEPGMVKNTYGTGCFLYMN
                     IGEELNIAGNGILTTVAWKIGDKVTYAYDGGVYIAGAAIQWLRDGIGVIKNYSETDDM
                     ANSISSTGGVYFVPAFAGIAAPYWDQYARGTMVGITGSTKKEHIVRATLESVALQVKD
                     VVDSMNLASGKKIKMLRVDGGITKNNFTMQFQSDILGIPIEIAENPETTVLGVGYMAG
                     LSCGVWNSIDEIRSEFTISKKYIPNMTNQKREEILLGWNQAVKRAMNWENDTKKVCAN
                     "
     gene            4250..5365
                     /locus_tag="L3M60_001941"
     CDS             4250..5365
                     /locus_tag="L3M60_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBN6226607.1"
                     /translation="MPFDKIILYIMAVGVLLGALDRIIGNKFGLGEQFEEGFNSMGPL
                     ALGMVGIVCLAPVISDVLGPIIIPIFNLCGADPSMFATILANDMGGYPLAMELAHNKE
                     AGMLAGLVVSSMLGCTLVFSIPVGLGLIEYEDRPFFAKGLLIGLITIPFGGVIGGLIA
                     GFNLSMVIINMIPVIILSVILAVGLKFNSTLMINGSLVFGKFISILITIGLGAAAFQE
                     ITGIVLIKGMTPIREGIQVIGSIAIVLLGTFPILHLLVKVLNKPLTIVGGKLGMDATS
                     AAGLVFTLANSIPVYKMMKDMSPKGKVVNTAWLVCATAALGDHLGFTAGVVPEMITPV
                     VIGKLVGGVLAIALAILMTKDLTLEEEQSIKMKKDAV"
     gene            complement(5646..6410)
                     /locus_tag="L3M60_001942"
     CDS             complement(5646..6410)
                     /locus_tag="L3M60_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN6226608.1"
                     /translation="MLPQERYEKILSILKSDNIIKINDIVQMFDISIETARRDLAHME
                     SIGLLKRVYGGAIPVIASSVELNYVKRKKINSNDKKQIAIKCSEFINTNDTIFMDTGT
                     TVLEVSKNLKDKKNLTVITNSILVANELMNTNIKVYVLGGYLRETEGSFSGPLTLSAI
                     EQFNVDKAILGAGGITLENGISDYHLEEALVRKKMIERSRKSIVVVEPPKFGINSFAS
                     VAPIEHIDTIITSASIDHNIIIEFSQKGIPLVIADN"
     gene            6669..6759
                     /locus_tag="L3M60_001943"
     tRNA            6669..6759
                     /locus_tag="L3M60_001943"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6703..6705,aa:Ser,seq:gct)
     gene            6847..7635
                     /locus_tag="L3M60_001944"
     CDS             6847..7635
                     /locus_tag="L3M60_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HBN6226609.1"
                     /translation="MTTLIFFIVGIASLVVAFVIDGGHVGALFVFSSALVVFGGTIGA
                     VGASTPFPVFKKSIKIMLLAFKNKKPNNIENIIYFKEIATKARKEGLLSIDKEISDNN
                     ELDPFIKKGLELMVDGVDAVTIRSILESQIQITSKRHKEGIAIFDSAGGYAPTMGIIG
                     TVMGLVHILGNLSSNPDALGPQISVAFLATLYGVGSANLIWLPIASKLKAINQQEISE
                     RLLVIEAILSILEGDNSNLMTEKLKIFLNSKELIELEEIGGRAE"
     gene            7656..8396
                     /locus_tag="L3M60_001945"
     CDS             7656..8396
                     /locus_tag="L3M60_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBN6226610.1"
                     /translation="MLYDEDEKEENNERWLLTYSDLITLLMIFFVIMYSMSNVDAEKY
                     KQLSQSLNSAFGGSSGVIEGGNSKIEPVVEPGSNDLDNLQNAKFKKVGEEIQKYLNEN
                     GMANSVSLRVQDRGLVISLKDTILFDTGKAVVKDNSREKIIQIGKMLNEMNSYIRVEG
                     HTDNMSIKNSEFKSNWDLSVMRATNVVQLLIDNAGIAPDKLSAVGYGEFRPIAENSSE
                     EGRSKNRRVDIVLVDSKYDNVENVSNDK"
     gene            8574..8942
                     /locus_tag="L3M60_001946"
     CDS             8574..8942
                     /locus_tag="L3M60_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226611.1"
                     /translation="MGNKNTKYKDKTRERYNKKVENLNKEKLNKANKESKKVDKIDDI
                     INKSSIVLCVLLVVFSLLYLFKLVQLNIIMILLVITVAIQQGLVSFGAFRNKNYVKAL
                     FSIFVIVCFLIAFKYVVNPV"
     gene            9034..10494
                     /locus_tag="L3M60_001947"
     CDS             9034..10494
                     /locus_tag="L3M60_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HBN6226612.1"
                     /translation="MNRCKFAKLIITLMIVLLLTGCSLKKEDNYKLDPENPISIQIWH
                     YYNGQQKMAFDKLVKEFNDTVGSERGIIVEAFSQGNVVDLSEKVLDSANKKVGSEDVP
                     NIFAAYSDTAYEMDKLGLLVDMDKYISKEELSEYIDEYVEEGRINSKGEFKLFPIAKS
                     TEILMLNKTDWDKFAKATNSNINDIKTIEGLVETSKKYYEWTDSLTKEPNDGKAFFGR
                     DALANYIIIGSKQLGVEIFSVKDGKVTLNIDEKVMKKLWDNFYVPYINGYFASYGKFR
                     SDDAKTGDILALVGSSSGAAYFPDRVMLNDNESYNIETSVFQAPRFNGEGKYAVQQGA
                     GMAVTKSTKEEEYASVEFLKWFTDKEPNIKFSIASGYLPVKKKTNDLEVIKEVMKKDN
                     LSLSDKVEGSIASSIEQINSCNLYTNKAFDGGTKARQILEYSMLDKAKEDLNKINELV
                     KSGTSKKDAVAQFNNDKNFETWFESFKAELEKAIKK"
     gene            10547..13576
                     /locus_tag="L3M60_001948"
     CDS             10547..13576
                     /locus_tag="L3M60_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6226613.1"
                     /translation="MENNLRKNNSIMKKMIFPLIIVMLIQTSLFCATILWGGTIKKLN
                     DNSFDILNERVINRKNYIQNEMLQRWSNTSETEARINSSVKKVLNEKNALIEDVGNNE
                     EINKDIIRIISNDLIYLLRKNSVTGAFIILSDKNIKDNPVDSNGEISRTGLYIRDLDP
                     KFNPNDNSDLLIERGSSDIARSFGISMDSYWEPKFSFKSENEQEGDDFFYKPFRAAID
                     NPNVSSSNLGYWGSKFLLTNAGDRIMTYTVPLIYEDGTVYGVLGIDLSVNYLRELLPY
                     SEINGNKKGNYILGVDRNDDMAFENVISNGYSLKEKFWNGLHSNLEQKESYKNIYKIK
                     NKDNECGNGNIYASVQYLNLYNSNTPFENERWALLGIIEEKSLLSFSKRVEKLVIISI
                     AISLLIGLIGIYVASRLFTKPIVLLANKVRNSNPSKPVNLDKINISEIDELSSAIELL
                     SANVADSSSKLSQIIEMVDMPIGAFELYKNADNVFCTKEFFSVFITESKFEEYGYMEK
                     SKFLKKLRGLDKYLQESYETENTYIYKFESKDKKPKWVRLNIFEDELKVLGVVVDVTK
                     EFIEKRKIEYERDFDILTNLLNRRAFHSQIQEKFKNKDELKVAAFIMWDLDNLKYIND
                     TYGHDAGDEYIRCAADILKKFISWNGIVSRLSGDEFYVFIYGYESKDSIRDIINSVKK
                     QMNNTLLKLHDGTEFKIRASAGISWYPDDSINYDELMRYADFAMYQIKNTIKGEVSEF
                     DINIYNKDSFLLSNREELNKLIDGQLVEYAFQPIVDCKGKVFAYEALMRPKIKTFSSP
                     IDVIRLARSQSKLYQIERITWFKALKSFKKYKEKFGDCKLFLNSIPNNNLSKVDFEEI
                     EDLYGEYLNRVVLEITENDKINEDFIEKKKAYIDHWNIEIALDDFGTGYNGDAVLLYI
                     MPDYVKIDMSIVRGIDKDENRQKILKNLISYSNERNIKVIAEGVETKGEMETLINLGI
                     DYMQGYYISKPSFIPEDISDKIKKELKICHENRNDVV"
     gene            13653..15719
                     /locus_tag="L3M60_001949"
     CDS             13653..15719
                     /locus_tag="L3M60_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HBN6226614.1"
                     /translation="MVELIITTNIDIRKLNENQLEAVEHIDGPCMILAGPGSGKTRVI
                     TYRIANMVVNKNIAPTRILAISFTKASSIEMKNRALNLSDDIRLNKVTYGTFHSVFFK
                     ILRYFERYNLDSIFDEKSKRMTIKAILKSLNIENADDDENIGQVINEISYVKNELMDK
                     NEFNSEVLTKDEFLKVYNLYEEQKSKVNKIDFDDMLIRTYYLLLKNKSALEMVRNVYK
                     YILIDEFQDINKVQFEVLKLISSPLNNIFAVGDEDQSIYGFRGARPDFLLEFEQYFNN
                     TKKIILDINYRSKSEIVHTANRLIDKNKNRYEKVIKCSQGDGGSVTYISPHDSEEEAL
                     YIAKEIIDEIKKDYVEYSDFAVIYRTNIQSRALVDVFMDMRIPFVVKDSVITIYDHWA
                     SQDILAYLRIGINPKSNKDWLRIINKPFRYISKDSINMVKDEKDFITALINKCNLHPK
                     QVKTINDLEIDLSYLNTLNPKNAISYIRTSLDYDRYVLDYCSNRKIKTNGLVEILNEL
                     ESSATNFNTVTEFLEHIDRVKTELTENNKNKQTEGVIFTTMHSAKGLEFRNVYIIGVN
                     EGTIPHEKSYDICKEEKKEEQLEEERRLMYVAITRAEEKLCISSTLNKYGKKVDKSLF
                     INDIKSPTKKEIDSIGVGDKVYHKKFHEGEIIKKDGIMFTIKFKDRERILDLKTCLLK
                     NIIYTI"
     misc_binding    16247..16457
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            16681..17496
                     /locus_tag="L3M60_001950"
     CDS             16681..17496
                     /locus_tag="L3M60_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBN6226615.1"
                     /translation="MKVFKKLLSLGLVLGLTLSLVGCSGGGEKTKLEQIKDSGKLVVG
                     TSAEFPPFEFHKVVDGKDSIKGFDIMLAEEFAKELGVKVEIKDMSFDGLIGALNADQV
                     DIVLAGMSPTPEREKSVDFSELYYLSRNAVIVKDADIDKVKTEDDLKKLRVGVQAGSI
                     QEEYVVNTLKMTTTKSLKAIPDLITELKNGNIDAVVTNEAVSLINVKKYDGIKMANTE
                     VGKNVTEGMAAAIKKADNNKEFIELLNKKIKELQDGKKIEEFLNEASTEAASN"
     gene            17524..18201
                     /locus_tag="L3M60_001951"
     CDS             17524..18201
                     /locus_tag="L3M60_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBN6226616.1"
                     /translation="MSLDFSFLSRFGTSFLEGTGVTVSISLVALCFGFIIGIIICMAK
                     ISKSKVLRAISSIYIEVLRGTPLLVQIYIVWFGLPQLGIRFPMLFGIPSEFIASAFAL
                     SVNSGAYVAEILRSGIQSVDNGQMEASRSLGLNYWSTMRYIIIPQAIKNILPSLANEF
                     ITLVKESSIISVIGVVEIMRTADIVKNAAFRALEPLIVAAAIYFVITFTLSRLVGLLE
                     KKLSVSN"
     gene            18260..18982
                     /locus_tag="L3M60_001952"
     CDS             18260..18982
                     /locus_tag="L3M60_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBN6226617.1"
                     /translation="MITIKNLSKSFGDLNVLKNIDLEIAKGEIMVIVGPSGSGKSTLL
                     RCMNLLEIPTGGEIIFEGKNLVDKKTNIDEVRQNIGMVFQNFNLFPHKTILDNITLAP
                     IKLKKMTKEEAEKKAEVLLSRVGLLDKKDSYPSQLSGGQKQRIAIARALAMEPDMMLF
                     DEPTSALDPEMVNEVLDVIKELAKEGMTMAIVTHEMGFAKEVADRVIFIDGGSILEDN
                     TPEEVFGNPKHERTKAFLAKVL"
BASE COUNT         7625 a         2001 c         3430 g         6245 t
ORIGIN      
        1 tttttaatta caaaactgtt gtaaaaaata taacgttatt taaaactttt ttttaatatg
       61 taaaaatata tactgatagg gggtatttgt ttttagaata ttcagtaaaa tagttttgag
      121 atattatata tattttgtat gttaaaaata aagaaattat tgttaaaaca gataaagacc
      181 ttaaattttt tatcgtaagc ttttaagtaa ataagacgta ttgaactaat taaaaatttt
      241 tattttgtaa aaatgagtat ttatttatat ggataaattt gattttaaat ctatcaatat
      301 agataaaatc tcattattta acttcaacta ttcctcaaaa aagtaacttt taaggaatag
      361 ttgaaacaaa aagtttggtt acataatagt aattttagtt acaagaatga aactgtaata
      421 cttaggattt tctttatata atttcattga gaacatttaa cttaggaggt aagatatgaa
      481 agtaaaaaaa tttaaaagtg gtatagtaat ggcattaaca gtaatggtaa ttggagcatc
      541 attgacaaat gtatcagcta tggaattaaa taagagtaag gagtttgaaa ctaaatacaa
      601 tgaaatgaag agggattttc aagaggaata tggaaaagaa gacaaattta atataacatt
      661 ggatgatgaa tttacaaaag aagaagtaaa tggagctaaa gggattataa agacaactaa
      721 taaagtgaat aatgaggttg tagtattaaa ttattttgat gaaatgaaat ctgggattga
      781 atttgagttg aagccaaaag cagaaaaaaa tgtaattttg agagaggaat ttcaacaagg
      841 ttatggaaag gaagataaat tcaaagttgt gttagatgat gaatttacaa aagaagaggt
      901 aaatggagct aagggaatta taaagactac agataaagaa acaggtgagg ttatcgtata
      961 taattacttt gatgagcttg aaaaataatg tgtaaagatt actttgattg aatgcaaagg
     1021 taattgtttt gagaatatga aatgactgaa taaaaaacga ttgtatcaaa aacaaataat
     1081 aattgaatgt aagtataatt gtatcaaaaa tataaaataa attaaaagaa aaagattata
     1141 ctgaaggaaa aagatttgaa ataggggtga actttacata ttatagttca cccttataca
     1201 aaaatataaa atataattta ttataggaaa atttaaaaaa atcagattat ttaatttata
     1261 ctgatatgtt gtaggatttt acaaacaaac tgttatataa ctttttttat ctccaatctt
     1321 gattttaata tatctaagaa tttataacaa tcttctggat atatttctcc catatttgca
     1381 acttggaata tgttagcatc taggaatttg ccttttcctg gatatacaac atagccactt
     1441 tccctttcta gccctaatat aaactcatct acattgattt ctttaggtag aaatacagat
     1501 gttactgtat tagacatata cttatctgga agtaaaaatg ttagacccaa ttcttccatg
     1561 ccattcctta aaatagcaga acattctttg tatctttcta ttttactgtc atcttccaaa
     1621 aactcagtta aagcttcatt aagggcaaat attaaagtaa cattaggtgt atttggtgtt
     1681 tgagaagatg atttagcaac tttatagtgt ttataaagat taagatacac attcttacat
     1741 tgattttctt taatatcttg taaaatactc tctttagcac aaaggtatgc agaacctgga
     1801 aatgctccaa gagctttacc accaacacct gttattatat ctatattaaa ctcttccaca
     1861 tcaatatatt cacctgctga tgcacttatt gcatctacaa aaaatatttt gttatacttc
     1921 ttagcaagtt ttcctagttc aggaaccgga tttatcattc ctgaactagt ttcatggaac
     1981 accatagcaa ttacttttat atcagaatgt tcaattatat atttttctaa aatgtttagg
     2041 tctgggtatt ctgcccattc aaaattaggc ttatatgttt ttacattata agtagataat
     2101 atttcttcta gtctttctcc aaaaacacca ttactaacta gcagaactgc atcttcttta
     2161 tcaaagatag aagatagtac acattcattt acagatgttc cagagccaga tacaattaat
     2221 gagtagtaat tagaagttgc attatatagt ttattgattt tttgttgtgt atctttgaat
     2281 aaatccataa actcatttcc tctatggcat atatcatagt gaagaagtga atttctgact
     2341 ttatctgtaa ccattactgg tccaggactg aatagatatg tttttttcat tgtaaagcct
     2401 ccttaaaaag ttaaatttaa atttttgaat gactgattat gtgtaaatat tagaatatta
     2461 tgtgttattt taactagatt atagaataaa ataataaata ttgcaataat attttaataa
     2521 aaatactttt ttaatcatgt taattaaaaa taacaaaaaa tattgtaatg aatattgcat
     2581 aaatccacat gatgatataa aatataaata acttgaatag attatatatt caaaaaataa
     2641 aaaagaattt attaattctg aaaataaagg gggacattta aagtgaaata tgtattagca
     2701 attgaccaag gaacaacagg aacaagagca gtattgataa acgaaaaatc agagtttgtt
     2761 tttagcgatt atatggaaca tactcaaata tatccacaat ctggatatgt cgaacatgac
     2821 cctatagaaa tttggaataa tacaaaaact gtgagtaata atgtattaca acaggctttt
     2881 gaaaatggaa tcaatgaaag cgatataaaa ggaattggta tagataatca aggtgaaaca
     2941 gtaatgttat gggataaaaa tacaggtaaa ccactttaca atgctattgt atggcagtgc
     3001 aggagaactt atgattatgt ggaaaattta aagaatatac caggtttaga aaataagata
     3061 atagataaga ctgggttaat tatagaccca tatttctcag gcacaaaaat taaatgggta
     3121 atagataatg taaaaggtgt aaaagaaaaa attaaaaatg gagaagtact tgcaggaaca
     3181 ttagatagtt ggttaatatg gaaaatgaca ggaggaaaat cttttttaac tgatgtatct
     3241 acagcagcaa gaacaatgct ttttaatata agagatatgg attgggataa agagttgctt
     3301 gacattatag gagtgcctag aagtatttta gcagatataa tgcctacaag tggtgatttt
     3361 ggaactacag ataaggaggc attttgtggg gtttctataa aaattactgc ttcaatggta
     3421 gaccaaccag cagctttatt tggcaatgga tgttttgaac caggaatggt taaaaatact
     3481 tatggtacag gatgttttct atatatgaat ataggtgagg aactaaatat agcaggtaat
     3541 ggcattttga caactgttgc ttggaaaata ggagataaag ttacatatgc ttatgatggt
     3601 ggagtgtata tagcaggtgc tgcaatacaa tggcttagag atggaattgg agttataaaa
     3661 aattattcag aaactgatga catggcaaat tccatatcaa gtactggagg tgtatatttt
     3721 gtaccagcat ttgcaggtat agcagcacct tattgggacc aatatgctag aggtacaatg
     3781 gttgggataa caggaagtac taaaaaagag catatagtaa gagcgactct tgagtctgtt
     3841 gcacttcaag tcaaggatgt tgttgattct atgaacttag cctcagggaa aaaaataaaa
     3901 atgttgagag tagatggtgg aattactaaa aataacttta caatgcaatt tcaatccgac
     3961 atattaggca taccaattga aattgcagaa aatcccgaga caacagtact tggagtagga
     4021 tatatggcag gtctttcctg tggtgtctgg aacagtattg atgaaattag aagtgaattt
     4081 acaataagca aaaaatatat tccaaatatg actaatcaaa aaagagaaga aatattactt
     4141 ggatggaatc aagcagtaaa aagagctatg aattgggaaa acgataccaa aaaagtgtgt
     4201 gctaattaag ttacaataga gtattttaca tgtataggag gaattaaaaa tgccctttga
     4261 taaaattata ttatacataa tggctgtggg agtgctttta ggagcgttag atagaataat
     4321 tggaaataaa tttggacttg gagagcagtt tgaagaaggg tttaattcaa tgggtccatt
     4381 ggctttagga atggttggta tagtttgttt agctccagta atttcagatg tactaggtcc
     4441 tataataata ccaattttca atctctgtgg agctgaccca tctatgtttg caactatttt
     4501 agcgaatgat atgggagggt atccattggc tatggaactt gcacacaata aagaggcagg
     4561 aatgcttgca ggattggtcg tgtcatccat gcttggatgt acattagtat tttcaattcc
     4621 agtaggacta ggtcttatag aatatgaaga cagaccattc tttgcaaaag ggttactgat
     4681 aggcctgata acaatacctt ttggaggtgt tataggagga ttaatagcag gatttaactt
     4741 atcaatggta attataaata tgataccagt gataatattg tcagtaatat tggcagtagg
     4801 tttaaaattt aattctacac ttatgataaa tgggtcatta gtttttggaa aatttatatc
     4861 tatattgata acgataggtt tgggagcagc agcatttcaa gaaataacag gtatagtact
     4921 tataaaaggc atgacaccta taagagaagg gattcaagta attggaagta ttgcaatagt
     4981 gttattaggt acttttccaa tattacattt attagttaaa gttcttaata aaccacttac
     5041 aatagtgggt ggaaaattag gtatggatgc cactagtgca gcaggattag tttttacttt
     5101 agccaatagt attccagtat ataaaatgat gaaagatatg agtccaaagg ggaaagtagt
     5161 aaatactgct tggttggttt gtgctacagc agctttggga gaccatttag gatttacagc
     5221 aggagtagtt ccagagatga ttacacctgt tgtaattgga aaattggttg gaggagtatt
     5281 ggcaattgca cttgccatac ttatgacaaa agatttaact ttagaagaag aacaaagtat
     5341 taaaatgaag aaagatgcag tgtagaataa ataataatta agtcaaattt aaactatttt
     5401 aaagtaggag tttaaataac taaaaataaa ttaaaaagac agacaattaa aaaataatcg
     5461 aatatccaaa acttataaat taaataaaat ggcaatcaaa taactaaaaa tgataagtta
     5521 actgaaagta attacataat taaaatataa agtatagttt agacataaat ggagctaaat
     5581 aaataattat ttggctcctt aattttaatt tattacatca aggaactaaa aatcaatcat
     5641 acaaactaat tatcagctat gactaaaggt attccttttt gagaaaactc tattattata
     5701 ttatggtcta tactagcaga ggtaataata gtatctatat gttctatagg agctactgat
     5761 gcaaatgaat ttataccaaa cttaggaggt tcgacaacta ctatagattt tctagacctt
     5821 tcaatcattt tttttctaac aagtgcttct tctaagtgat agtcagagat tccattttca
     5881 agagtgatac ctccagcacc taaaatagct ttatcaacat taaattgttc aatagctgac
     5941 agtgtaagcg gaccagaaaa agaaccttca gtttctctaa gatagccacc aagcacatat
     6001 acttttatat tagtattcat aagttcgttt gcaactaaaa ttgagttggt tattacagtt
     6061 agattctttt tatctttcaa attttttgaa acttcaagta cagtagttcc agtatccata
     6121 aaaatagtat catttgtatt tataaactca ctacatttaa tagcaatctg ttttttatcg
     6181 tttgagttta ttttttttct tttaacatag ttaagttcta cgctacttgc tataactggt
     6241 atagcaccac cataaactct ttttaaaaga ccgatactct ccatatgggc taaatctctt
     6301 cgtgcagttt ctattgatat atcaaacatt tgaactatgt catttatttt aattatatta
     6361 tctgatttga gaatacttaa aatcttctcg tatctttcct gtggtaacat aaaatatcct
     6421 ccttggacta gtattatttt agttttggca taacttacaa ttaagtcaga gtttaggtat
     6481 atttgttaat gtatgtatat ttatcaaata atattataag tgttatgcaa gataatttta
     6541 ccatatttaa aacttacatt taagcaaaaa agtaaaaata gatgtttaat aattcaaaat
     6601 aactcaaatt ttataaaaca attgactaaa atatgatttt attatataat ttattagtat
     6661 agagatatgg agaagtactc aagtggctca agaggatccc ctgctaaggg attaggctgg
     6721 gtaaccggtg cgagggttcg aatccctcct tctccgccaa ttgttaaagg ctatttttat
     6781 taaagatagt ctttttatga tgtcataaat gattagatat gtataactaa agaaaggagg
     6841 gaatagatga caactttaat ttttttcata gtggggatag catctttggt agttgctttt
     6901 gtaattgatg gggggcatgt tggagcgtta tttgtatttt catcagcact cgttgttttt
     6961 ggtggaacta taggagctgt tggagcttcg acgccattcc ctgtatttaa aaagtctata
     7021 aagataatgt tattagcttt taaaaataaa aagcctaata acatagaaaa tataatctat
     7081 ttcaaagaaa ttgcaacaaa ggctaggaaa gaggggcttc ttagtattga caaggaaata
     7141 agtgataata atgaattaga cccttttatt aaaaaagggc ttgaactgat ggtagatgga
     7201 gtggatgcag ttactataag aagcatattg gaatcacaga tacagataac atcaaaacga
     7261 cataaagaag gaattgcaat ttttgattca gcaggtggat atgcacctac aatggggata
     7321 ataggtacag ttatggggtt agtacatatt ttaggtaatc tttcaagtaa tccagatgca
     7381 ttaggacctc aaatttctgt tgcatttttg gctactttgt atggtgttgg ttctgccaat
     7441 ttaatatggt taccaatagc atcaaaactt aaggctataa atcaacagga aataagtgaa
     7501 agacttttgg tgatagaagc aatattatcc atacttgagg gtgataattc taatttaatg
     7561 acagaaaaat taaaaatatt tttaaattca aaagaactaa ttgaacttga agaaattggt
     7621 ggtagagctg aataatataa aaggatggaa tgtttatgtt gtatgatgaa gatgaaaaag
     7681 aagaaaacaa tgagcgatgg cttttaactt attcagacct cataacatta cttatgattt
     7741 tctttgtaat aatgtattct atgagtaatg ttgatgcgga gaaatataaa caattatctc
     7801 aatctctaaa ttctgctttt ggtggttctt ctggagttat tgaaggtggt aatagtaaaa
     7861 tagaaccagt agttgaacca ggaagtaatg atttagataa tttacaaaat gctaagttta
     7921 aaaaagttgg tgaagaaatc cagaagtatt taaatgaaaa tggtatggct aattcagtat
     7981 cattaagagt tcaagataga ggattagtaa taagtcttaa agatacaata ctatttgata
     8041 cggggaaagc agttgtaaag gacaattcca gagagaagat tattcaaata ggtaagatgt
     8101 taaatgaaat gaatagttac attagagtag aaggtcatac agacaatatg tctataaaaa
     8161 attcagagtt taagtcaaat tgggatttat cagttatgag agcaaccaat gttgttcaac
     8221 ttcttattga caatgcaggt atagcaccag ataaattgtc tgctgtagga tatggtgagt
     8281 ttagaccgat agcagaaaat tcatctgaag aaggtaggtc aaaaaataga agagtcgata
     8341 tagttttagt tgatagtaaa tatgataatg tagaaaatgt ttcaaatgat aaatagttaa
     8401 attagctgtc tcaacacaga attaattcta aaggggatgg cttttttatg cgaaaattaa
     8461 aaatagatag atagtaagtt acctttttgt ggtaatatat ggtatcttaa atatataata
     8521 tttaaataat atataagctc aattaaaaaa attttataga ggatgggaaa tgaatgggaa
     8581 ataaaaatac gaaatataaa gataaaacaa gagaaaggta taataaaaaa gttgagaatt
     8641 taaataaaga aaaattaaat aaagctaata aagaatctaa gaaagtagat aagattgatg
     8701 atattatcaa taaatctagc attgtattat gtgtacttct agtagtgttt tcattacttt
     8761 acttattcaa attagttcaa cttaatataa taatgatact tttagtaatt acagtagcta
     8821 ttcaacaggg attggtgtca tttggtgcat ttagaaataa aaattatgta aaagctttat
     8881 tttctatatt tgtaattgta tgtttcttaa ttgcttttaa atatgttgta aatcctgttt
     8941 aaatatatca ataaacgttc tataattaat ataaatagtg aagttatagt tttaatgcta
     9001 ttgtaaattt tataatttga aggagtagat tttatgaata ggtgtaagtt tgcaaaatta
     9061 attatcacat taatgatagt tttattactt acaggatgta gtttaaaaaa ggaagataat
     9121 tataagctag acccagaaaa tccgatatca atacaaattt ggcattatta taatggacag
     9181 caaaaaatgg cttttgataa acttgttaag gaatttaatg acacagtagg ttctgaaaga
     9241 ggaattattg ttgaagcatt cagtcaaggt aatgtagttg atttatcaga gaaagtttta
     9301 gattctgcaa ataaaaaagt tggctctgaa gatgtaccaa atatatttgc agcatactca
     9361 gatactgcat atgaaatgga taaattaggg ctactagtag atatggataa gtacataagt
     9421 aaagaagaat taagtgaata tatagacgaa tatgtagaag aaggtagaat aaattctaag
     9481 ggtgagttta aacttttccc aattgcaaaa tcaactgaaa ttttgatgtt gaataaaact
     9541 gattgggata aatttgcaaa agctacaaat tcaaatataa atgatataaa aacaattgaa
     9601 ggattagtag agacatcaaa aaaatattat gaatggacag atagtttaac gaaagagcct
     9661 aatgatggta aagcgttttt tggaagagat gctttagcaa attatattat tataggaagt
     9721 aaacaactag gagtagaaat tttttctgtt aaagatggta aagttacatt aaatattgat
     9781 gaaaaagtta tgaaaaaact atgggataat ttctatgtac catatataaa tggatatttt
     9841 gcttcttatg gtaagtttcg ttctgatgat gcaaaaacag gtgatatatt agctcttgta
     9901 ggctccagtt caggagcagc atactttcca gatagagtaa tgctaaatga caatgagagt
     9961 tataatattg aaacatctgt ttttcaagcg ccacgtttta atggagaagg aaaatatgct
    10021 gtacaacaag gtgcaggaat ggctgtaaca aaatctacta aagaagaaga atatgcatca
    10081 gtagaatttt taaaatggtt tacagataaa gaacctaata taaaattctc tattgcttct
    10141 ggatacttac cagtaaagaa aaaaacgaat gatttagagg ttataaaaga agttatgaag
    10201 aaagataatt tgagcctatc tgataaagtt gaaggctcaa tagcttcatc tattgaacaa
    10261 attaacagtt gtaatttata tactaataaa gcatttgatg gtggaactaa agctagacaa
    10321 atacttgaat attcaatgct tgataaagca aaagaagatt taaataaaat aaatgaatta
    10381 gtaaaaagtg gaacttctaa gaaggatgca gtagcacaat ttaataatga taagaatttt
    10441 gaaacttggt ttgaaagttt taaggctgag cttgaaaaag ctataaaaaa atagatgaat
    10501 tggcagttag tgtgtcgaca agcacaaagg tataggtgga ataagtatgg aaaataatct
    10561 aagaaaaaac aattcaataa tgaaaaagat gatatttccc ctaattattg ttatgctaat
    10621 tcaaacaagt ttattttgtg ccacaatttt atggggagga actattaaga aattaaatga
    10681 taattcgttt gacatcttaa atgaaagagt aattaatcgt aaaaattata tacaaaatga
    10741 aatgttacaa agatggtcaa atacaagtga aacagaagct agaattaatt cctctgtaaa
    10801 aaaagtctta aatgaaaaaa atgctttaat tgaggatgta ggcaacaatg aagaaattaa
    10861 caaagacatt ataagaatca tatcaaatga tttaatatat cttttgagaa aaaattctgt
    10921 tacaggagct tttataatat taagtgacaa aaatattaaa gataatccag tagatagtaa
    10981 tggtgaaata agtaggactg gattatatat aagggattta gaccctaaat ttaatccaaa
    11041 tgataattct gatttgttaa ttgagagagg ttcatcagat atagcaagaa gttttggaat
    11101 atctatggac agttattggg aacctaaatt ttcatttaaa agtgaaaatg aacaagaagg
    11161 agatgatttt ttttataaac catttagagc tgcaattgat aatccaaatg tatcaagttc
    11221 taatttggga tactggggaa gtaagttttt attgacaaat gcaggtgata ggattatgac
    11281 atatacagtt ccacttattt atgaagatgg aactgtatat ggagtacttg gaatagattt
    11341 aagtgtaaat tatttaagag aattacttcc atactcagag ataaatggaa ataaaaaagg
    11401 taattatatt ttaggtgtgg atagaaatga tgatatggca tttgaaaatg taatttcaaa
    11461 tggatactca ttaaaagaaa agttttggaa tggtttacat tctaatttag agcagaagga
    11521 atcgtataaa aatatatata agattaaaaa taaagataat gaatgtggta atggcaatat
    11581 ttatgctagt gtacaatact taaatttata taattccaat actccatttg aaaatgaacg
    11641 ttgggctctt ttgggtatta ttgaagaaaa aagtctttta tctttttcaa aaagggtaga
    11701 gaaattagtt ataatatcta tagcaatttc tttattaata ggtctaatag gtatttatgt
    11761 tgcatctaga ttatttacaa aaccaattgt tcttttagcc aacaaagtta gaaacagtaa
    11821 tccatcaaaa ccagtaaatc ttgataagat aaatatatct gaaatagatg aattatcttc
    11881 agctatagaa ttactcagtg caaatgttgc agattcctca tcaaaactat cgcagattat
    11941 tgaaatggta gatatgccaa ttggggcttt tgaattatac aaaaatgcag ataatgtttt
    12001 ttgtacaaaa gaatttttta gtgtttttat tactgaaagc aaatttgaag aatatggtta
    12061 tatggaaaaa agtaagttcc ttaaaaaatt aaggggatta gataaatatt tgcaagaaag
    12121 ttatgaaaca gaaaatacat atatatacaa gtttgaatct aaagataaaa agcctaagtg
    12181 ggtaagactg aatatctttg aagatgagtt aaaggttctt ggcgttgttg ttgatgtcac
    12241 aaaagagttt attgagaaaa gaaagataga atatgagaga gattttgata ttttaacgaa
    12301 tcttttaaat aggagagctt ttcattcaca gatacaagaa aaatttaaga ataaagatga
    12361 acttaaggta gcagcattca ttatgtggga tttagataat ttaaagtata ttaatgatac
    12421 atatggacat gatgctggtg atgaatatat tagatgtgct gcagacatac ttaagaagtt
    12481 tatatcgtgg aatggtatag tatctagact ttcaggtgat gagttttatg tatttatata
    12541 tggatatgaa agtaaagata gtataagaga tattattaat tctgttaaga aacagatgaa
    12601 taatacctta ttaaaacttc atgatggaac agaatttaaa ataagggcat cagcaggaat
    12661 ttcctggtac cctgatgatt caatcaacta tgatgagtta atgagatacg cagattttgc
    12721 tatgtatcaa attaaaaata caatcaaagg tgaagttagt gaatttgata ttaatatata
    12781 taataaggat tcattcttat tgagcaatag agaagaatta aacaagttaa ttgatggtca
    12841 actagttgaa tatgcatttc agcctattgt agattgtaaa ggtaaagtat ttgcatatga
    12901 agcacttatg agacctaaaa taaaaacttt tagttcacca atagatgtca ttagattagc
    12961 tcgttcacag tctaaattat atcaaattga gcgtattaca tggtttaagg cacttaaatc
    13021 atttaaaaaa tacaaagaaa aatttggtga ttgtaaactg ttcttaaatt ctattccaaa
    13081 taataattta tcaaaagtag attttgagga aattgaagat ttatatggtg aatatttaaa
    13141 tagagtagta ttagaaataa cagagaatga taagattaat gaagatttta ttgagaaaaa
    13201 gaaagcatat atagaccatt ggaatataga aattgctttg gatgactttg ggacaggtta
    13261 taatggagat gctgtactat tatatataat gccagactat gtaaagattg atatgtctat
    13321 tgtaagaggt attgataaag atgaaaatag acagaaaata ttaaaaaatc ttatctcata
    13381 ttccaacgaa agaaatatta aagtgatagc agaaggtgta gagacaaaag gtgaaatgga
    13441 aacactaatt aatcttggaa tagattatat gcagggttac tatattagta aaccatcatt
    13501 tatacctgaa gatataagtg ataaaattaa aaaagagctc aagatatgtc atgaaaatag
    13561 gaatgatgtg gtttaataga ggaaaaatta aatttatttt tatgaacttt tagtttataa
    13621 ttaaaaagaa aataatataa ttataaatta aggtggtgga gctaataatc acaacaaata
    13681 tagatataag aaagttaaat gaaaatcaat tagaagcagt tgagcatata gatgggcctt
    13741 gcatgatact tgcagggcct ggttcaggta agactagagt aattacatat agaatagcca
    13801 atatggtcgt aaacaaaaac atagcaccaa caagaatttt ggcaataagt tttaccaagg
    13861 catcttcgat agaaatgaaa aatagagctt tgaatttaag tgatgatata agacttaata
    13921 aggtaaccta tggtactttc cactctgtat tttttaaaat tctaagatac tttgaaagat
    13981 ataatttaga tagtatattt gatgaaaaat caaagcgaat gacaataaag gcaatcttaa
    14041 aaagtttaaa tatagagaat gctgatgatg atgagaatat aggacaagtt ataaatgaaa
    14101 tttcctatgt aaaaaatgaa cttatggata aaaatgaatt taattcagaa gtattaacaa
    14161 aagatgagtt tttaaaagta tacaacttat atgaagaaca aaaatctaaa gttaataaaa
    14221 tagattttga tgatatgctt ataagaactt attatttact tttgaaaaat aagtctgcac
    14281 tagaaatggt tagaaatgtg tataaatata tattgataga tgaatttcaa gatataaata
    14341 aagtacagtt tgaagtttta aagttaattt ctagcccatt gaacaatata tttgcagttg
    14401 gagatgaaga ccaaagtata tatggattta gaggtgctag accagatttt ttgcttgaat
    14461 ttgaacagta ctttaataat actaaaaaaa taatattaga tataaattac aggtctaaaa
    14521 gtgagatagt gcatactgca aatagactta tcgacaaaaa taagaataga tatgaaaaag
    14581 ttattaagtg tagtcaaggt gatggaggaa gtgttaccta tatttctcca catgattcag
    14641 aggaagaagc cttatatatt gccaaggaaa taattgacga aataaaaaaa gattatgtag
    14701 agtattcaga ttttgcagtg atttatagaa ctaatataca gtcaagagca ttagtagatg
    14761 tatttatgga tatgaggatt ccatttgtag taaaagattc agtaattaca atatatgacc
    14821 attgggcaag tcaagatata ctagcttatt taagaatagg tattaatcca aaatcaaata
    14881 aagattggtt gagaattata aataagcctt ttagatatat atccaaagac agtataaata
    14941 tggttaagga tgaaaaagat tttataacag cattaataaa taagtgtaat cttcatccta
    15001 aacaagttaa aacaataaat gatttggaaa tagatttaag ctatttaaat actttaaatc
    15061 ctaaaaatgc aatatcttat attagaacta gccttgatta tgatagatac gttttagatt
    15121 attgtagcaa cagaaagata aagactaatg gacttgtaga gatattaaat gaacttgaga
    15181 gctcagcaac taattttaat actgttacag agtttttaga gcatattgat agagtaaaga
    15241 ctgagttaac ggaaaataac aagaataagc agactgaagg tgttatattt acaactatgc
    15301 atagtgcaaa aggattagaa tttagaaatg tttatattat aggtgtaaat gaaggaacaa
    15361 tacctcatga aaaatcatat gatatttgta aggaagagaa aaaagaagaa caattagaag
    15421 aagaaagaag acttatgtat gttgctatta caagagctga agaaaaatta tgtataagct
    15481 ctactttaaa taagtatggt aagaaagttg ataaatcatt atttattaat gatataaaat
    15541 ctccaacgaa aaaagaaatt gatagtattg gtgtagggga taaggtatac cataaaaaat
    15601 ttcatgaagg agaaataatt aaaaaagatg gaattatgtt tacaataaag tttaaagata
    15661 gagagcgaat cttggatttg aaaacttgtt tattgaagaa tataatttat actatttaat
    15721 ttagaaccta aatttagata caatgtatta tttatatgga tagactagta agaagtatat
    15781 tctttattgt gtgtaagtaa aagagatatg tgtaaaatgt attatttaag ttattagaaa
    15841 agagaaataa acgtttaata tattatttat agttgttaag aaaaagcaaa actgtgaaag
    15901 atattattta gcattattta agaaaaagca aaactgtgaa agatattatt tagcattatt
    15961 taagaaaaag caaaactgtg aaagatatta tttagcatta tttagtaaaa agcaaaactg
    16021 tagaagatat tatttagaat tattatagta aaaagcaaaa ctgtagaatg tattatttaa
    16081 gaaatagcta aaaatataaa atatactatt tgtaattata taaagaatat caataaatat
    16141 acacagtagt atttatataa tttacaagca aaaattatta aaaaaataaa aaacttattg
    16201 acaaaaatat tataaggaag tacaattgta ttaacaattt aaggaaacaa atccagtgat
    16261 agagaaagta gcatatatgg atttacagcg agttagggat tggtgtaagc ctagcaatga
    16321 agtttatgtg aagagagctc tggaggagat tatcagaaat tttagtatga taattggtct
    16381 gcccgcctta agggattgag aggataagta cttacttatc aaaaagagtg gtaacgcgga
    16441 tataattcgt ctcttagctg taaagctaag ggactttttt gatttaaaaa tcaaaatatc
    16501 catatagcta aagctaacaa tcacaaattt tgaaagtaat agaagtattt atcaataaga
    16561 gtggtaacgc ggtaaagtcg tctcttagtc attaaagact gagagacttt ttttgtaact
    16621 cgaaacattt aagtattaaa atttgaggga aattataatt aaaagagagg ggtagttaaa
    16681 atgaaagtat ttaaaaaatt attaagttta ggattagtat taggattgac attatcgcta
    16741 gtaggatgta gcggtggagg agaaaagaca aagttagaac aaataaaaga cagtggaaaa
    16801 ttggttgttg gtactagtgc agaatttcct ccatttgaat ttcacaaggt agtagatgga
    16861 aaagattcaa taaaaggatt cgatataatg ttggctgaag aatttgcaaa agaacttggt
    16921 gtaaaagttg aaattaagga tatgtcattt gatggattga taggtgcttt aaatgcagac
    16981 caagttgata ttgttcttgc aggtatgtct ccaacaccag agagagaaaa gagtgttgat
    17041 ttctcagaat tatattattt aagtagaaat gcagttatag ttaaagatgc agatatagac
    17101 aaagtaaaaa ctgaagacga tttaaagaaa cttagagttg gagttcaagc aggaagtatc
    17161 caagaagaat atgtagttaa cactttaaaa atgacaacta caaaatcttt aaaagcaata
    17221 cctgatttaa ttacagaatt aaaaaatgga aacatagatg cagttgttac taatgaagcc
    17281 gtttctttaa taaatgttaa aaaatatgat ggaataaaaa tggcaaatac tgaagttgga
    17341 aaaaatgtaa cagaaggtat ggctgcagca atcaaaaaag ctgacaataa caaagagttt
    17401 atagaactat taaacaaaaa gataaaagaa ttacaagatg gtaagaagat agaagaattt
    17461 ttaaatgaag catctactga agctgcttca aattagtaag aaagttatgt gggagatgaa
    17521 attatgagtt tagattttag ttttttaagt agatttggga cttctttttt ggaaggaaca
    17581 ggtgtgacag tatcaatttc tcttgtggca ttatgctttg gatttataat aggtataatt
    17641 atctgtatgg caaaaatatc aaagagcaaa gtattaagag caatatcatc aatctatatt
    17701 gaggttttaa gagggactcc attattagta caaatatata tagtatggtt tggtttacct
    17761 caattaggaa taagattccc tatgttgttt ggtataccaa gtgaatttat agctagtgca
    17821 tttgcattat cagtaaactc tggtgcatat gtggcagaga tacttagatc aggcatacaa
    17881 tcagttgata atggacaaat ggaagcaagt agatcattag gattaaatta ttggtcgact
    17941 atgagatata taataatacc tcaagctata aagaatatat taccttcatt agcaaatgag
    18001 tttattacac ttgtaaaaga atcttcaata atatctgtaa taggagttgt tgagataatg
    18061 cgtactgctg atatagtaaa aaatgcagca tttagagcat tagagccatt aatagtagca
    18121 gcagcaattt actttgttat aacatttact ctatcaagat tagttggatt attagaaaag
    18181 aaactatctg tgtctaacta gatgagagca tgtttagcat ttagggaaaa catagaatag
    18241 taaaaaggaa agtgataata tgattacaat aaaaaattta agtaaatctt ttggagattt
    18301 aaatgtatta aaaaacatag acttagaaat cgctaaaggc gaaataatgg ttatagtagg
    18361 cccaagtggt tctggtaaaa gtacacttct tagatgtatg aatttactag aaatacctac
    18421 aggtggagaa ataatatttg aaggaaaaaa tttggtcgat aaaaaaacta atattgatga
    18481 ggttagacaa aatataggta tggtgtttca gaactttaat ttattcccac ataagactat
    18541 tttagataat ataacattag caccaattaa attaaaaaag atgactaagg aagaagcaga
    18601 aaaaaaagca gaagttttgt tgagtagagt tggtctttta gataaaaaag attcatatcc
    18661 atcacaatta tctggaggac aaaagcaaag aatagcaata gctagagcac ttgctatgga
    18721 accagatatg atgttgtttg atgagccaac atctgctctt gacccagaaa tggtaaacga
    18781 agttttggat gttataaaag aacttgctaa agaaggtatg acaatggcaa ttgtaactca
    18841 tgagatggga tttgctaagg aagttgcaga tagagttatt tttatagatg gaggaagtat
    18901 acttgaagat aatactccag aagaagtttt cggaaatcca aagcatgaaa gaacaaaagc
    18961 atttttagca aaagtgttat aatatataaa aaggatgtta ttgacattag aaattcaata
    19021 catccttttt taatgatggt taatataaat tttaaaacta aagttatatt aatattaact
    19081 ttagtttttc aaatctttgc aaaaaaaaaa gagctcgggg taagctcttt ttttccaccg
    19141 aattaatttc cgttactaat ccgattttta ataaatataa aatatatcct tatgaaaggg
    19201 tattgggaat agatatatta actaactagg gttaccaggg ggataacctt aatttattat
    19261 aacaaaatgc gacacaaaaa tcaatatttt ttttggaata a
//
LOCUS       DAFNNB010000065        18932 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0066, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000065 DAFNNB010000000
VERSION     DAFNNB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18932
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0066"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(393..809)
                     /locus_tag="L3M60_001953"
     CDS             complement(393..809)
                     /locus_tag="L3M60_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6226618.1"
                     /translation="MAALKSFEKPLTPEEEIEYLTKFKIENDKSAKDTLIERNMRLVA
                     YIAKKYNNSTEDQDDLISIGTIGLIKAIETYNIDKGTRLATYASRCIENEILMNIRSN
                     KKNKTQVSLQDPIGTDKEGNEIHTTFYMLFSTIIYI"
     gene            1376..1738
                     /locus_tag="L3M60_001954"
     CDS             1376..1738
                     /locus_tag="L3M60_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226619.1"
                     /translation="MKIRKRGFISIECVISIAILYVAVYLVSTSLYSCYSFVSRNISD
                     RKMLSTAKKYIEDEKYRIQNSKYELIENKIEKNYINGYEISSRVEQILDYYQCYEINI
                     EIKNEFKKLRFNSYVTRK"
     gene            1722..2441
                     /locus_tag="L3M60_001955"
     CDS             1722..2441
                     /locus_tag="L3M60_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226620.1"
                     /translation="MLLENKGSILIITLIIFSIISTVCIMCIGLIYTHNNMFKLGYKD
                     IQLKEISLSGIEISVSNVLSCVEEAIDISNSEGEFREYFLGNNMIECIKKIKDTSYSS
                     VEGLSLKVENNTIYDREDFFEFDIESSVREKEFLKTIKVRVKIKNPWVGIDIEDYMNK
                     SNNNKIYDKKFNNYKASENNEILNQFKNESENISNQEVVEPTEEDNEEYEEELKQNII
                     ENIKKNFDEKKLVQVYKYRGV"
     gene            2461..2880
                     /locus_tag="L3M60_001956"
     CDS             2461..2880
                     /locus_tag="L3M60_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226621.1"
                     /translation="MTVIELIVSISIILLIVTLSFPKDNIENHKINLFARQLCSDIRY
                     VRRINMFSNYNTYIYYINQGKHEGYVLRKDGKNIKSIILPKNVKIARSIETIKFKSDG
                     SPQKASTIEIYNNRLRKIITITPVSGRVLLKEGKYET"
     gene            2870..3400
                     /locus_tag="L3M60_001957"
     CDS             2870..3400
                     /locus_tag="L3M60_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226622.1"
                     /translation="MKHKYGYLLLESVVSLSSMLIMILVLYSIFLSTISLKLKVEDKI
                     ELQQQSLEIIKSMEGIISNSMGIINVSNYEDTFKKATSIKCRYVDENVNNNEESISNK
                     EIILNERRNKLFVNSLNGESSQAGGYEIGDYVDEMYVSINNNGQYVNIKLKLSKRSQK
                     YETEFKIKVWNFSENI"
     gene            3713..4270
                     /gene="efp"
                     /locus_tag="L3M60_001958"
     CDS             3713..4270
                     /gene="efp"
                     /locus_tag="L3M60_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBN6226623.1"
                     /translation="MVSAGDFRKGVTFEKDGQPCLVVDFQHVKPGKGAAFVRTKYKNL
                     KTGAIREESFNPSEKFPKAVIDTRQMQYLYNDGELYYFMDQENFEQIPLNYDQVEDAI
                     KFLKENEVATIRFYQGQPFQVEAPNFAELEVTDTEPGIKGDTASNVTKAATVETGAVV
                     QVPLFINTGDKVKIDTRTGEYLSRV"
     gene            4362..4817
                     /locus_tag="L3M60_001959"
     CDS             4362..4817
                     /locus_tag="L3M60_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBN6226624.1"
                     /translation="MKHLYEEVAYLKGLAEGLEISAESKEGKMIHKIVDALEVFAEAI
                     VTLDEEQEELQDFVESIDEDLADLEEDIADMEEDLYEEDDDEDDEDFSYIEMECPNCG
                     ELVEIDEDLLYDDEVDVVCPDCKAVILSSEDDCDDDCTCGGCSSCDDRE"
     gene            4979..5689
                     /locus_tag="L3M60_001960"
     CDS             4979..5689
                     /locus_tag="L3M60_001960"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBN6226625.1"
                     /translation="MKISKKLLDNIQRFENVINYKFKNKEYILEALTHSSYSNENKKY
                     NFNERLEFLGDSVLGIVISDYLFNEEANLPEGELTKLRANIVCEDSLSEVANDINLGV
                     HMLLGRGEEATGGRHRTSILADAFEAVIAAIYLDGGFESARQFILHHMENIIYDSRKG
                     NIFRDYKTHLQEVLQGNGENNIWYRLIEEKGPDHNKRFVMEVGINEDVLGIGEGKSKK
                     EAEQLAAKIALKKKLWEK"
     gene            5743..6813
                     /locus_tag="L3M60_001961"
     CDS             5743..6813
                     /locus_tag="L3M60_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6226626.1"
                     /translation="MKKRIIPIFVPHKGCPHDCIFCNQKKITGVSTDVTSEDARNIIE
                     ECLETIDKDADVEIAFFGGSFTAIDIDIQKSLLSVAKEYVEKGLVKDIRMSTRPDCID
                     EDILSMLKEYKTSIIELGVQSLDEKVLLDSIRGHQSEIVYKSSKMIKNSGIKLGLQMM
                     IGLPTDTEEKCIFTAKKFVELKPDCVRVYPTLVVKDTGLEKLMEQNKYSPFTLEESVN
                     IVKKVLALFYVNNINVIRVGLQATDDIQIGKAVLAGPYHPAFRELVEADMIKDYLEFV
                     ILQNKNIKQMLVKSNKKNISKIIGNKKTNVKYMEEKLGVILKTQESDLDINQLEIVLD
                     GKSLISANMRDIHRKLYDIYDL"
     gene            6841..10395
                     /gene="smc"
                     /locus_tag="L3M60_001962"
     CDS             6841..10395
                     /gene="smc"
                     /locus_tag="L3M60_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HBN6226627.1"
                     /translation="MYLKRLELKGFKSFPVKTDIIFKEGITAIVGPNGSGKSNISDAV
                     RWVLGEQSIKSLRGDKLEDVIFAGTDTKKPMNYCEVALTIDNSENQLELDFTEVTIRR
                     RAYRNGESEFFLNNKSCRLKDIKEVFLDTGIGKDGYSIIEQGKVDEILSNNPLSRRKV
                     FDEACGISKYRYKKQEAERNLSNTKENLERIDDIYIEIENQLKPLFNQQTKAKKYLEI
                     SEKLKILEVNSFIREIEGIEKELSEVNEHRKVIEKELNEKEEQKNVVEKKQEDINKEV
                     EVLQDVIEKSVDYINSIKGVISKKESQINLIKERIRNFTNEISRKNLEINDIKEKLNE
                     NKQYIKELENNKLSSSGELSALQENIKVLEASKDKQKIKLESLNNEIELLKESIIDIL
                     NKKQEFSNKLSTLNANKENMNVRDENINLEITELNKNIDIKSSELDTINKEFNMQNEN
                     LKNVNNRHKELSTNLQDSISEHSKLEDEIQKSKYNLNGYNSKLNVYIDMENHYEGFNR
                     GVKEVLKNKNLKGVHGALGQIINVPEKYEKSIEAALGAYMQNIITDNEFSAKSAINYL
                     KQNNLGRVTFLPLNIIKSNKISLGNLKANTKFIGIASDLITFDEKYRNIIENILGRTI
                     LINNIDEGIKFAKETGHRFKIVTLDGEILNPGGSLTGGSLKTNGNILSRKRYINEYTE
                     KINNIKHEISQLELKRESLDKDIKNIKSEIDSHESKIKDLEKSIIIKSTSIENIESEI
                     ESLKGSITKLENEKNDLNSNLNYTLEKRDNVRKDIEELDNLYNQNKEKIDALNEEIKR
                     YNDLYDKEKSEFDELNLSLVKKTEAYNSIVRDIKRISGENYELEEKNKQLEESLNYEE
                     QEIIKLQDSIATEEKEKENLTKQLGDSNRNLETRKIAKDDLKNSFDEINKELKTIDRQ
                     HIELKESLFKVGGRLERLKTSQDTYINKLFEQYDMTLVQALEIKDEDLDIDRKFLESL
                     KREIRNLGNINIDSIKEYEEIKERYDFYSEQKQDLEESMEEIEKLIHTLEENMKSEFE
                     IKFEEISKNFKYVYKRLFGGGCGELTILDKENLLESDILITAQPPGKKMKNLNLLSGG
                     EKALTAISILFAILITKPTPFCILDEIEAPLDDANIFRFGEFLKDLSKETQFISVTHR
                     RGTMEAADYIYGVTMQEKAISKVISLKLKEAKEITDII"
     gene            10422..11693
                     /gene="ftsY"
                     /locus_tag="L3M60_001963"
     CDS             10422..11693
                     /gene="ftsY"
                     /locus_tag="L3M60_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBN6226628.1"
                     /translation="MLKKLFGFGKDKEKEIEKKDEEEVVDSVDSLENLEETIFSGLEE
                     EVIDKDEDIEEKTEESVNNEGTEEIENFEKIDVEMDSDGKEVENISDNDSQEEIIEEL
                     ENYDEIESKEIEAKEEKKVNLFERLKQGLTKAKQGITDRIDEVLKSYTKIDEELLEDL
                     EEILITADVGVNTTMDIIERLRDKIKQKGIIEPIKVREELKSIVEDILTNENSTLDIE
                     PAPCIILMVGVNGVGKTTTIGKLANRYKKDGKKVLLAAADTFRAAATEQLEIWANRTN
                     VDIIKHQEGADPGAVVFDAIKAAKARKTDVLICDTAGRLHNKANLMNELGKVFKIVDR
                     EFPEAKREVLLVVDATTGQNAVIQAKTFKEVADITGIVLTKLDGTAKGGVVLAVKSEV
                     DVPVKLIGVGESVEDLQDFNAKSFSDALFGN"
     gene            11796..12164
                     /locus_tag="L3M60_001964"
     CDS             11796..12164
                     /locus_tag="L3M60_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HBN6226629.1"
                     /translation="MNIEKMVEIGLLFEQYKELLTDKQKEIVALYYEEDYSLGEISEN
                     LNVSRQGVYDTLKRSEKILRDYEEKLHLVSKIQEQEKNIKIIKDKIIDIKEDLLHNRD
                     CANLIPKLENIEDVCREMIK"
     gene            12161..13513
                     /gene="ffh"
                     /locus_tag="L3M60_001965"
     CDS             12161..13513
                     /gene="ffh"
                     /locus_tag="L3M60_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBN6226630.1"
                     /translation="MIFEGLSDKLQGAFSKLKSKGKLTEADVKNAMREVKLALLEADV
                     NFKVVKDFIKKVQERCVGQEVMQSLTPAQHVIKIVNEELTSLMGDVQSKVMISSKPPT
                     ILMMVGLQGAGKTTTAGKLGGYFKKQGKKPLLVACDIYRPAAIKQLQVVGEKLDIPVF
                     NMGDKENPVNIAKAGLSHAIKNANDLVIIDTAGRLHIDEVLMDELKSIKSEVKPHEIL
                     LVVDSMTGQDAVNVAESFNEALGVDGVVLTKLDGDTRGGAALSIRAVTQKPIKFMGMG
                     EKLDDIEPFHPDRMASRILGMGDVLSLIEKAQENIDLEKAKELEQKIKKQELDFEDFL
                     QQMEQIQNMGPLDKILGMIPGMGNVKDQLGDVDLNGKEMKRTKAIIQSMTTYERRNPG
                     VLNASRKKRIARGSGTSVQDVNRLIKQLNEMKKMMKMFAGSQKSMKKRGGLGGLPFFK
                     "
     gene            13551..13823
                     /gene="rpsP"
                     /locus_tag="L3M60_001966"
     CDS             13551..13823
                     /gene="rpsP"
                     /locus_tag="L3M60_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBN6226631.1"
                     /translation="MAVKIRLKRMGANKKPFYRIVVADSRAPRDGKFIEEIGYYNPIS
                     EPKQVRINDEKAIKWLATGAQPTEVVKKLLVKNGVIEKFEASKQAK"
     gene            13888..14115
                     /locus_tag="L3M60_001967"
     CDS             13888..14115
                     /locus_tag="L3M60_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HBN6226632.1"
                     /translation="MKELVVDIAKALVDNPDSVVVEEFEDNDGIVLKLTVAQEDMGKV
                     IGKQGRIAKAIRTVVRSVANRENIKVSLEIV"
     gene            14223..14738
                     /gene="rimM"
                     /locus_tag="L3M60_001968"
     CDS             14223..14738
                     /gene="rimM"
                     /locus_tag="L3M60_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA processing protein RimM"
                     /protein_id="HBN6226633.1"
                     /translation="MKEKLTHFKVGQIVNTQGLKGEVRVYPLTDDIERFDELKDFYLG
                     KDLNNKLSVEKVRYKGSMVIMKIKGIDSIEKAEKLKNKFMYVSREDSRELEEGEFFIA
                     DMIGIEVLTVDGKYVGILEDVLQYSANDVYVVKGEEDKEFMIPAIKKFVPTIDIDERK
                     MIIDPIKGMID"
     gene            14747..15442
                     /gene="trmD"
                     /locus_tag="L3M60_001969"
     CDS             14747..15442
                     /gene="trmD"
                     /locus_tag="L3M60_001969"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBN6226634.1"
                     /translation="MRFHIMTLFPEIFNSYMDESIMKRAVEKGIIEVHIYNIRDFSNN
                     KHKKVDDYPFGGGAGMVMTPQPIYDTYKHIITTHNINNPSVIYLTPKGKVYNQSIAKH
                     MSLKEDIILLCGHYEGIDERIIDLIVTDEISIGDYVLTGGELPALIMIDSISRLIPGV
                     LNQEESFEEESFKDNLLEYPHYTRPRDFEGLKVPEVLLSGNHKKIDEWRREESIRITK
                     ERRFDLYKKSNEK"
     gene            15616..15966
                     /gene="rplS"
                     /locus_tag="L3M60_001970"
     CDS             15616..15966
                     /gene="rplS"
                     /locus_tag="L3M60_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBN6226635.1"
                     /translation="MNEMLRAIEQEQLKNEVPNFGPGDTVKVHVRIIEGKRERIQVFE
                     GVVLKRQGGGARETFTVRKMSFNVGVERTFPVHSPKIEKIEVTRKGKVRRAKLNYLRG
                     RVGKAAKIKEARNK"
     gene            16288..17184
                     /gene="ylqF"
                     /locus_tag="L3M60_001971"
     CDS             16288..17184
                     /gene="ylqF"
                     /locus_tag="L3M60_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HBN6226636.1"
                     /translation="MSNEYEEYLMDDNLHINWYPGHMKKTKELVKNNLKLIDVVIELL
                     DARIPFSSKNPDIDRLVGDKPRVVVLNKSDMADKDKLNQWIEYYKKINIKAIPVDTIK
                     GVGINKIIEECKNVTREKMSSLKDKGRKERAIRIMIVGVPNVGKSSLINKLTGRKSTQ
                     TGDKPGVTKGKQWVRLKGNLELLDTPGILWPKFEDQEVALNLAFSRAIKDEILDTETL
                     ALKLIEKLMKIEPEKLKARYKLDCLGETPIETMDMIGHKRGFITGKKELDYTRIATTV
                     LNEFRDGKIGNITLEIPENVKR"
     gene            17573..18865
                     /gene="brnQ"
                     /locus_tag="L3M60_001972"
     CDS             17573..18865
                     /gene="brnQ"
                     /locus_tag="L3M60_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBN6226637.1"
                     /translation="MGKTKDIIVFGFALFAMFFGAGNLIFPPYLGIITGPEWLIAFLG
                     FTFADAGLALLAVMATAKFDGNVVEMFKRCGMKLGILIGCADILCIGPFLAIPRTGAT
                     TYEMGIMPLFGTSIPVLLFCILFFAISYVLTIKPSKVVDIVGQFLTPALLIALAFIII
                     KGIISPLGDIVDKPMIPNVFAEGIGQGYQTMDAFAAIALASVLIVSLNDKGYSTISDK
                     LKMIGKAGVLACGGLALVYGGLCFLGATVSTMYGTDAVQSQVIVNITEGLLGNVGKVI
                     LAVVVSLACLTTSIGLTSATGQYFSRLTKGKLSYEKIVLAVSVFSAVVASFGVGTIIK
                     IASPILSIVYPPSIVLIILAFFNEKIKNDNVYKGAVYMSLLVSILTVISSYGVAVPVV
                     NSLPLHSLGFNWVVPVIIAGIIGNFIPSKSQSNTLGTN"
BASE COUNT         8000 a         1767 c         3470 g         5695 t
ORIGIN      
        1 ttattataat gcattttaat aaaaaaataa aggttatgag taaaatattt atagaaaaaa
       61 gtatattatt gaattgatta taaagtttta ttatttttat cattcttaaa tacacattaa
      121 tatctataaa atgacaaaat aaaatatact atttaaatag atattaaaaa attgatgcca
      181 agtatttgtt tgctatatga ataacaattt atttaggagc tttgatttga cactgtataa
      241 tgataacgta attgttaaaa aagtttgctg atttggatgt gtttttagaa aatagaaaaa
      301 tatatactta ctgactaatg ataattttat atgtatttta gtttgagtga tttaagaaaa
      361 gtgttttgaa gggggagtat tttatagagt aactaaatat atataatagt agaaaataac
      421 atataaaaag tagtgtgtat ttcattacct tctttatctg ttccgatagg gtcttgtaga
      481 gatacttgag ttttattttt tttgttagac ctaatattca ttaaaatttc attttcaata
      541 catctagaag catatgttgc taatcgagtt cctttatcta tgttataagt ttctatagct
      601 ttaattaatc ctattgtacc tatagatatt aaatcatcct ggtcttctgt tgaattatta
      661 tatttctttg ctatataagc tactaatctc atgtttcttt caattaatgt atcttttgca
      721 cttttatcgt tttctatttt aaatttagtt aaatactcaa tctcttcttc aggggttaag
      781 ggtttttcaa aagatttaag agctgccaat aggtcacccc cttttcaacg gtcactttaa
      841 attatatgaa ggagacttat ccaagttgcc tgttatctaa taaaataata tgcatatttt
      901 tagaatttaa aacatatatt tgcaaacttt tagatggaat atgacataaa gagtgggaat
      961 ttaaatttaa gtttttgttg tatatatatg aaacaaggcg aaggaattat tgatttataa
     1021 agtataaatt gaaactgagg tgtcaataaa tataaaggaa atagtattta tgtatgtttt
     1081 gatgttgagg taaaaaatta atgaaaaaca ttagaatcta taaaaaatat gaatatagga
     1141 ggtgtaattt tgcaataatt aaaaaagtaa atgcaattaa ttatttggat aaagttgatt
     1201 gcaagtaaaa agtattagtc atagttaata ctgttaaggg tgaaggtggt gtgttaatct
     1261 gtatgtgtgt aggaagagtt ttgagttata atgaaaaaac ttataaaagg tatttatgaa
     1321 aaacttatag ttaaccatct atatataaaa tattatttag gggttgtttt tattattgaa
     1381 aataagaaag cgtggtttta tatcaatcga atgtgtaata agtattgcta tattatatgt
     1441 ggctgtttat ttagtttcta catcattgta tagttgttat agttttgtca gtagaaatat
     1501 atctgacaga aaaatgttaa gtactgcaaa aaaatatata gaagatgaga agtatagaat
     1561 acaaaatagt aagtatgagt taattgaaaa taagatagaa aaaaattaca taaatggata
     1621 tgaaattagc agtagagtag agcaaatttt agattattat caatgctatg aaataaatat
     1681 agagataaaa aatgaattta aaaaattgag gtttaatagc tatgttacta gaaaataaag
     1741 gctccatatt aataataaca ttgataatat tctctattat aagtactgtg tgtattatgt
     1801 gtataggttt aatttataca cataataata tgtttaagtt aggatacaag gacattcaac
     1861 taaaagaaat ttctttaagt ggaatagaaa tatcagtaag taatgtactt tcttgtgttg
     1921 aggaagctat tgatatatct aatagtgaag gtgaatttag agaatatttc ttaggaaata
     1981 acatgattga atgtataaaa aaaataaaag atacttctta tagtagcgta gaaggacttt
     2041 ctttaaaagt agaaaataat actatttatg atagagaaga cttttttgaa tttgatatag
     2101 aaagtagtgt tagagaaaaa gaatttttaa aaacaataaa agtaagagtg aaaattaaaa
     2161 atccatgggt aggaattgac attgaagatt atatgaataa atctaataac aataaaattt
     2221 atgacaaaaa atttaataat tataaagcat ctgaaaataa tgaaattcta aatcaattta
     2281 aaaatgaaag tgaaaatata agtaatcaag aggttgttga acctacagaa gaagacaatg
     2341 aggaatatga agaagaactt aaacaaaaca taattgaaaa tataaaaaag aattttgatg
     2401 aaaaaaaact agtacaggta tataagtata ggggtgtata actttgaaaa aagaaggaat
     2461 gtgactgtga tagaattaat agtatctatt agtataatac tcttgatagt tacattatct
     2521 tttccaaaag ataatattga aaatcataaa ataaatttat ttgcacgtca actttgttca
     2581 gatataaggt atgttaggcg aattaatatg tttagtaact acaatacata tatatactat
     2641 ataaatcaag gtaaacatga aggttatgta ttgagaaaag atggtaaaaa tattaagtca
     2701 ataatactgc ctaaaaatgt aaaaatagct agaagtattg aaacaataaa gtttaaatct
     2761 gatgggtcac ctcaaaaggc atctacaata gagatatata ataatagatt aagaaaaatt
     2821 ataacaataa cacctgtaag tggaagagta cttctgaaag agggaaaata tgaaacataa
     2881 atatggttac ttacttttgg aaagtgtagt atcattgtcc tcaatgttga taatgatact
     2941 agttttatat tctatatttt tgtctacaat aagcttaaaa ttaaaagtag aagataagat
     3001 agaactacaa caacaaagtc ttgaaatcat aaaatctatg gaaggaatta ttagcaattc
     3061 tatgggaata ataaatgtat ctaattatga agacacattt aaaaaggcaa catctataaa
     3121 atgtagatat gtagatgaaa atgtaaataa taatgaagaa agcataagca ataaagaaat
     3181 tatattgaat gaaagacgaa ataagttatt tgtaaattct ttaaatggcg aaagtagtca
     3241 agcaggtggt tatgaaatag gagattatgt agatgagatg tatgtatcaa tcaataataa
     3301 tggccaatat gttaatatta aattgaaatt atcaaaacgt agtcaaaaat atgagactga
     3361 gtttaaaata aaagtatgga attttagtga aaatatataa aaatacataa atttatagag
     3421 aaagaaataa tatcaataag tcatataaaa tataattatt gagaactata taaataaata
     3481 atatcaatat gtaaagtgca tagattaaga atatagagga taataataaa aatatagaat
     3541 aagtaatttg ccaaatactt gtttatattg tataacttat gatattatga tattagtaaa
     3601 aatttgtaat cctgacttga cagaattcaa gtaagagata taattttaaa ggcatagtaa
     3661 tgtaaaattt actaaaaaat aagaaattta ttttaatatg gaggaacaaa aaatggtatc
     3721 agcaggtgat tttagaaaag gtgttacatt tgaaaaagat ggacaaccat gtttagtagt
     3781 tgattttcaa cacgttaagc caggtaaagg agctgctttc gttagaacta aatacaaaaa
     3841 cttaaaaaca ggagctataa gagaagaaag tttcaatcca agtgaaaaat tccctaaggc
     3901 tgttatagat acaagacaaa tgcaatatct atacaatgat ggtgagttat attattttat
     3961 ggatcaagaa aattttgaac aaataccatt aaattatgat caagttgaag atgcaattaa
     4021 gttcttaaaa gaaaatgaag ttgctacaat aagattttac caaggacaac cattccaagt
     4081 ggaagctcca aactttgcag agctagaagt tacagataca gagccaggta taaaaggtga
     4141 tacagcaagt aatgtaacta aagcagctac agttgaaact ggggcagtcg ttcaagttcc
     4201 attatttata aatactggag ataaagtaaa gatagatact agaacaggtg aatatttatc
     4261 aagagtataa aaatttatgt atataaaagc ttatatgtct taataatata agatatatga
     4321 gcttttagtt tatatataaa aaagaaagcg aggggtgtta catgaaacat ttatatgaag
     4381 aagttgcata cttaaaaggc ctagcagaag gactagaaat aagtgctgaa agtaaagaag
     4441 ggaaaatgat acataaaata gttgatgcat tagaagtatt tgcagaggct attgtaacat
     4501 tggatgaaga acaagaagaa cttcaagatt ttgttgaatc tatagatgaa gatttagcag
     4561 atttagaaga agatattgct gacatggaag aagatcttta tgaagaagat gacgatgagg
     4621 atgatgagga cttcagctat atagaaatgg aatgcccaaa ttgtggagag ttagtagaaa
     4681 tagacgaaga tttgttatat gatgatgaag tagatgttgt ttgtcctgat tgtaaggctg
     4741 tgatattatc atcagaagat gattgtgatg atgattgtac atgtggtggt tgttcaagtt
     4801 gtgatgatag agagtaaaaa ttcataagaa ttaaaactgc ctatgatgcc tatgagatat
     4861 tttgcttcat aggtagtttt atttttatga ggaaggtatt atgtattttg gttttgcaat
     4921 acttggacaa aaatagtaca ctagaattta tagaatagga ttttggaggg atgtaaatat
     4981 gaaaataagt aaaaaattgc tagataatat acaaagattt gaaaatgtta taaattataa
     5041 atttaaaaat aaagaatata tactagaagc tcttactcat agttcatatt ctaatgaaaa
     5101 taaaaaatat aattttaacg aaaggttgga atttttagga gattcggttt taggcatagt
     5161 gataagtgat tatttgttta acgaagaagc taatctgccc gaaggggaac taactaaact
     5221 tagagcaaat atagtttgtg aagattcgtt aagtgaagta gctaatgata taaatttagg
     5281 tgtgcacatg ttattaggta gaggagaaga agcaacagga ggaagacata ggacttcaat
     5341 attagcagat gcatttgaag ctgtaatagc tgctatatat cttgatggag gatttgaaag
     5401 tgcaagacag tttatacttc atcatatgga aaatataatt tatgattcaa gaaaaggaaa
     5461 tatatttaga gactacaaaa cgcatcttca agaagtgtta caagggaatg gagaaaataa
     5521 tatctggtac aggcttatag aagaaaaagg accagaccat aataaaagat ttgtaatgga
     5581 agttggaatt aatgaagatg tattgggtat aggagaaggc aaaagtaaaa aagaagctga
     5641 acagttggct gctaaaatag ctttaaagaa aaaattatgg gaaaaataaa tataatatgt
     5701 aaaaatataa aaagaaataa ttatattaga aggatgttgt agatgaaaaa gagaattata
     5761 cctatatttg ttcctcacaa gggatgtcct catgattgca tattttgtaa tcaaaaaaaa
     5821 ataacaggtg tgagtactga tgttactagt gaagatgcaa gaaatataat tgaagaatgt
     5881 ttagagacta tagataaaga tgcagatgta gaaatagcat tttttggtgg tagctttact
     5941 gctatagata ttgatataca aaaaagttta ttatcagttg ctaaagaata tgtagaaaaa
     6001 ggtcttgtaa aagatattag aatgtctaca agaccagatt gtattgatga agatatatta
     6061 agtatgctaa aagaatataa gacaagtata attgaactag gagtacaatc tttagatgaa
     6121 aaagtattat tagatagtat aagaggtcat caatctgaaa ttgtatacaa gagttcaaag
     6181 atgataaaaa atagtggaat taaactcgga ttacagatga tgattggtct tccaactgat
     6241 acagaagaaa agtgtatatt tacagcaaaa aaatttgtag agctaaaacc agattgtgtt
     6301 agagtttatc caactttggt agtaaaagac acaggacttg aaaagttaat ggaacaaaat
     6361 aaatatagtc catttactct tgaagagagt gtaaacatag taaaaaaagt tcttgcactg
     6421 ttttatgtta ataatattaa tgtaataaga gttgggcttc aagctactga tgatatacaa
     6481 attggaaaag ctgttttagc aggaccatat catcctgcat ttagagagtt agtagaagct
     6541 gatatgataa aagattattt agaatttgtt attttgcaaa ataaaaatat aaagcagatg
     6601 ttggtcaaat ctaataaaaa gaatatttct aaaatcatag gaaataaaaa aacaaatgtt
     6661 aaatatatgg aagaaaaact tggtgtgatt ttaaaaacac aagaatcaga tttagatata
     6721 aatcaattag aaattgtgtt agatggaaaa agtttaatta gtgctaatat gagagacatt
     6781 catagaaaat tgtatgacat ttatgacctt tagttttaat aatgaaggga gagaaaagat
     6841 ttgtatttaa aaagactaga gttaaaggga tttaaatcct ttccagtaaa aacagatatt
     6901 atttttaaag aaggaataac agccatagta ggaccaaatg ggagtggaaa aagtaatata
     6961 tctgatgctg taagatgggt attaggagaa cagagtatta agagccttag aggtgataaa
     7021 ctcgaagatg taatatttgc aggtacagat acaaaaaaac ctatgaatta ttgtgaagtg
     7081 gctcttacta ttgataattc tgaaaatcaa ttggaattag attttacaga agtaactata
     7141 agaagaaggg cttatagaaa tggtgaaagt gaattttttc taaataataa aagttgcaga
     7201 ttaaaagata taaaagaagt ctttttagat actggtattg gaaaagatgg ttattcaatc
     7261 atagaacaag gaaaagtaga tgaaatatta agtaataatc ctttaagcag aagaaaagtt
     7321 tttgatgaag cttgtggcat atcaaaatat agatataaaa aacaggaagc agaaaggaat
     7381 ttaagcaata caaaggaaaa tttagaaaga atagatgata tatatataga aattgaaaat
     7441 caattaaaac ctctttttaa tcaacaaacc aaggcaaaaa aatacttaga aataagtgaa
     7501 aaattaaaaa tacttgaagt aaatagcttt ataagagaaa ttgaaggaat agaaaaagaa
     7561 ttaagtgagg ttaatgaaca tcgtaaggtt attgaaaagg aactcaatga aaaagaagaa
     7621 caaaaaaatg ttgtagagaa aaagcaagaa gatattaata aagaggtaga ggtattacag
     7681 gatgtaattg aaaaatctgt agattatata aattcaatca aaggagttat atcaaaaaaa
     7741 gagtcccaaa taaatttaat taaggaaaga ataagaaatt ttacgaatga aattagtaga
     7801 aaaaatttag aaataaatga tataaaagag aaactaaatg aaaataagca atacataaaa
     7861 gaattagaaa ataataaatt atcaagtagt ggagaactat ctgccttgca ggaaaatata
     7921 aaagtattgg aagcaagtaa agataaacaa aaaattaagt tagaatccct aaataatgag
     7981 atagaattat tgaaagaaag tataattgat atattaaata agaaacaaga gtttagtaat
     8041 aagttgtcca ctttaaacgc aaataaagaa aatatgaatg ttagagatga aaatataaat
     8101 ttggagataa cagaactaaa caaaaatatt gatataaaat catctgaatt agatactata
     8161 aataaagagt ttaatatgca aaatgaaaat ttaaagaatg taaataatag gcataaagaa
     8221 ttatcaacta atttgcaaga ttcaattagt gagcacagta aactagaaga tgaaatacag
     8281 aaaagtaaat ataatctaaa tggatataat tcaaaattaa atgtctatat agatatggaa
     8341 aatcattatg aaggatttaa tagaggagta aaagaagtac taaaaaataa aaatttaaaa
     8401 ggtgtacatg gagcattagg acaaattata aatgtacctg aaaaatatga gaaatctata
     8461 gaagcagcac ttggtgctta tatgcaaaat attataactg ataatgagtt tagtgctaag
     8521 tctgcaataa actatctaaa acagaataat ttgggtagag taacatttct tccattaaat
     8581 ataattaaat caaataaaat aagcctagga aatctaaaag caaatactaa gttcatagga
     8641 atagcaagtg atttaattac atttgatgaa aaatatagaa atataattga aaatatactt
     8701 ggaagaacaa tacttataaa taatatagat gaggggataa aatttgcaaa agaaactgga
     8761 cataggttta aaatagtaac tttagatggt gaaatcttaa atccaggagg ttctttgact
     8821 ggaggaagtc taaagactaa tgggaatata ctatcaagaa aaagatacat taatgaatat
     8881 acagaaaaga taaataatat caagcatgaa atttcacaat tagaattaaa aagagaatct
     8941 ctagataaag atataaaaaa tataaagagt gaaattgatt cacatgaaag taaaataaaa
     9001 gatttagaaa aaagtataat aataaaaagt acaagcatag agaatattga atctgaaatt
     9061 gaatcgttaa aaggtagtat tactaaatta gagaatgaga aaaatgattt aaattctaac
     9121 ctaaattata cacttgaaaa aagagataat gttagaaaag atatagaaga gttagataat
     9181 ttatacaatc aaaataaaga aaaaattgat gcattgaatg aggaaataaa aagatacaat
     9241 gatttatatg ataaagaaaa atctgaattt gatgaattaa acttaagctt agtgaagaaa
     9301 acagaagctt acaatagtat agttagagat ataaaaagaa tatctggtga aaattatgag
     9361 ctagaagaaa agaataaaca attagaagag tcattaaact atgaagaaca ggaaataatt
     9421 aaattacaag attctatagc tactgaagaa aaagaaaaag aaaatctaac aaaacaacta
     9481 ggagatagta atagaaatct tgaaactaga aaaatagcaa aagatgattt gaaaaatagt
     9541 tttgatgaaa ttaataaaga attaaaaact atagatagac agcatataga gcttaaagaa
     9601 agtttattta aagttggtgg aagattagaa agattaaaaa ctagtcaaga cacatatata
     9661 aataagttgt ttgagcaata tgatatgact ttagtacaag cattggaaat taaagatgaa
     9721 gatttggata tagatagaaa attcttagaa agtctaaaga gagaaataag aaatcttgga
     9781 aatataaata tagactctat aaaggaatat gaggaaataa aagaaagata cgatttttat
     9841 agtgaacaaa agcaagattt agaagagtct atggaagaga tagaaaaact tatacatact
     9901 ttagaagaaa atatgaaatc tgaatttgaa attaagtttg aagaaataag taaaaatttt
     9961 aaatatgtat ataagagatt atttggtggt ggttgtggag aattaactat cttagataaa
    10021 gaaaatttat tagaaagtga tatactaata acagctcaac ccccaggtaa aaaaatgaaa
    10081 aatttaaacc tcttatctgg tggagaaaaa gctttaacgg caataagtat attatttgct
    10141 atattgatta caaagccaac accgttttgt atattagacg agattgaagc tccacttgat
    10201 gatgcaaata tatttagatt tggagagttt ttgaaggatt tatctaaaga gactcaattt
    10261 atatcagtaa ctcataggag aggaactatg gaagctgctg attatatcta tggagttaca
    10321 atgcaagaaa aagccatttc aaaagttatt agtttaaaat taaaagaagc taaggaaata
    10381 accgatatta tataacttag attgacatag gaggaataac agtgttaaaa aaattatttg
    10441 gttttggaaa agacaaggaa aaggaaatag aaaagaaaga tgaagaagaa gtagtagaca
    10501 gtgttgatag tctagaaaac ttggaagaaa ccattttttc aggacttgaa gaagaagtga
    10561 tagataaaga cgaagatatt gaagaaaaaa ctgaggaaag tgtcaataat gaaggcactg
    10621 aagaaatcga aaactttgaa aaaatagacg ttgaaatgga tagtgatgga aaagaagtag
    10681 aaaatatttc agataatgat tctcaagaag agataatcga agaattagaa aattatgatg
    10741 aaattgagtc taaagagata gaagctaaag aagaaaagaa agttaacttg tttgaaagac
    10801 taaaacaagg attgacaaaa gcaaagcaag gaataactga tagaatagat gaggttttaa
    10861 aatcatatac aaaaatagat gaagaattac ttgaagattt agaggaaatt ttaataacag
    10921 cagatgttgg tgtaaataca actatggata taattgaaag actaagagat aaaataaagc
    10981 aaaaaggtat tattgaacca atcaaggtaa gagaagaact taaatctata gttgaagata
    11041 ttcttacaaa tgaaaactct acactagata tagaaccagc accatgtata atattaatgg
    11101 tgggtgtaaa tggggtcggt aagactacta caataggtaa gctagcaaat agatacaaaa
    11161 aagatggtaa aaaagtttta ttagcagcag cagatacatt tagagctgca gcaacagagc
    11221 aattagaaat ttgggcaaat agaaccaatg tagacataat aaaacaccaa gaaggagcag
    11281 accccggagc tgtagtattt gatgctataa aagccgcaaa agctagaaaa acagatgtat
    11341 taatatgtga tacagcaggg agattacaca ataaggctaa tcttatgaat gaacttggaa
    11401 aagtatttaa aatagtggat agagaatttc cagaagcaaa gcgtgaagta cttcttgtgg
    11461 tagatgctac aacaggtcaa aatgcagtta ttcaagcaaa aacctttaaa gaagttgcag
    11521 atataacagg tatagtactt acaaaacttg atggaactgc aaaaggtggc gtagtgcttg
    11581 cagtaaaatc agaagtagat gtgcctgtaa aacttattgg agttggagaa agtgtggaag
    11641 acctacaaga tttcaatgca aaatcatttt cagatgcatt atttggtaac tagtgttctt
    11701 ttaaaaataa tggttgacaa gagtgttttt atgatataaa atacaatagt gtaaagtaaa
    11761 taacctttac agtatactgc taggaagtgt aagatatgaa tatagaaaaa atggtcgaaa
    11821 taggattgtt atttgaacag tataaagaat tattaactga caagcagaaa gaaatagttg
    11881 ctctatacta tgaagaagat tattctttag gtgaaattag tgagaatctg aatgtatcaa
    11941 gacaaggtgt gtatgatacc ttaaagcgtt cagaaaaaat actaagagat tatgaagaga
    12001 agttacactt agtatcaaag attcaagagc aggaaaaaaa tattaagatt ataaaagata
    12061 aaattattga cattaaagaa gatttattgc acaatagaga ttgtgctaat ttaatcccta
    12121 agctagaaaa tatagaagat gtatgtaggg agatgataaa atgatatttg aaggattgtc
    12181 tgataaacta caaggggcat ttagtaagtt aaaatctaaa ggaaaactta cagaagcaga
    12241 tgtaaaaaat gctatgagag aagttaaact tgctcttctt gaggcagatg ttaactttaa
    12301 agtagttaaa gatttcataa aaaaagttca agagagatgt gttggacaag aagttatgca
    12361 aagcttaact ccagctcagc atgttatcaa aattgtaaat gaagagctta caagtctaat
    12421 gggagatgta caaagtaaag taatgatttc ctcaaagcct cctacaattt taatgatggt
    12481 tggattgcag ggtgcaggta agactactac agcaggaaaa cttggaggat attttaaaaa
    12541 gcaaggtaaa aaacctctat tagtagcttg tgatatttat agaccagcag ctataaaaca
    12601 acttcaagtt gttggagaaa aattggatat acctgtattc aatatgggag acaaagaaaa
    12661 tccagtaaat attgcaaaag caggtttaag ccatgcaatt aaaaacgcta atgatttagt
    12721 tattatagat acagctggta gacttcatat agatgaagta cttatggatg agttaaagtc
    12781 tattaaatca gaagttaagc cacatgagat acttttagtt gtagattcta tgacaggtca
    12841 agatgctgta aatgttgcag aaagcttcaa tgaggcactt ggtgttgatg gagtagtatt
    12901 aactaaattg gatggagata ctagaggtgg tgctgcactt tcaataagag cagttacaca
    12961 aaagcctata aaatttatgg ggatgggaga aaagctagat gatatagaac ctttccaccc
    13021 agatagaatg gcatcgagaa tattaggtat gggagatgtc ttaagcttaa tagaaaaagc
    13081 gcaagaaaac atagacttag agaaagctaa ggaattagag caaaaaatta aaaaacaaga
    13141 gcttgatttt gaagatttct tacaacaaat ggaacaaata caaaatatgg gacctcttga
    13201 caagatactt ggtatgatac ctggtatggg aaatgtaaaa gaccaacttg gagatgtaga
    13261 tttgaatggt aaagaaatga agagaacaaa agccataatt cagtcaatga ctacatatga
    13321 gagacgaaac ccaggagtat taaatgcttc aagaaagaaa agaatagcta gaggcagtgg
    13381 aaccagtgta caagatgtaa atagacttat aaaacaactt aatgaaatga aaaaaatgat
    13441 gaagatgttt gctggttcgc aaaagagtat gaagaaaagg ggcggcttag gcggtttacc
    13501 tttttttaaa taaaatataa aaaagtaaaa attattcgga ggtgactgta atggcagtta
    13561 aaataagatt aaaaagaatg ggagctaata aaaaaccttt ctacagaata gtagtagctg
    13621 attcaagagc tccaagagat ggaaaattta tagaagaaat aggatactat aatccaattt
    13681 ctgagcctaa acaagtaaga ataaacgatg aaaaagctat aaaatggtta gctactggtg
    13741 ctcagccaac agaagtagtt aaaaaattac ttgtaaaaaa cggagtaata gaaaaattcg
    13801 aagcttctaa acaagcaaaa taattttttc ttggcaatca gtttaagtaa gtactcattg
    13861 ctaaggttac tttaggagat gaaatatatg aaagagctag ttgtagatat agctaaggct
    13921 ctagttgata atcctgactc agttgttgtt gaggaatttg aagataatga cggtatcgtt
    13981 ttaaagctta cagttgctca agaagatatg ggtaaggtta ttgggaagca gggaagaata
    14041 gctaaagcta taagaactgt tgtaagatct gttgcaaata gagaaaatat aaaagtttct
    14101 cttgagatag tataaaatag gaggattagg tataatgcct agtccttttt atataaattt
    14161 gtactttaag attttatgat gatatgacca cataagatta aattttttaa gaaggtgata
    14221 acatgaaaga aaaattaact cattttaagg taggtcaaat agtaaataca caaggtctaa
    14281 aaggtgaggt aagagtttat ccacttactg atgatataga gaggtttgat gaattaaaag
    14341 atttctattt gggaaaagat ttaaataata aattgtctgt tgaaaaagta agatacaaag
    14401 gttctatggt tataatgaag ataaaaggta tagatagcat agagaaagca gaaaaactaa
    14461 aaaataaatt tatgtatgta tctagagaag atagcagaga gcttgaagaa ggcgaatttt
    14521 ttatagcaga tatgattgga atagaagttt taaccgtaga tggaaaatat gtaggaatat
    14581 tagaagatgt tcttcagtat tcagcaaatg atgtttatgt agtaaaaggt gaggaagata
    14641 aagaatttat gataccagct ataaaaaaat ttgtacctac aatagatatt gatgaaagaa
    14701 aaatgattat agaccctatt aagggaatga tagattaggt ggtaatatga gatttcatat
    14761 aatgacactt tttccagaaa tatttaattc gtatatggat gaaagtataa tgaagagagc
    14821 agttgaaaaa ggtataattg aggttcatat atataatata agggattttt ctaataataa
    14881 acataaaaaa gtagatgatt atccatttgg aggaggagct ggaatggtaa tgactcctca
    14941 acctatatat gacacgtata agcatataat aacaactcat aatataaata atccaagtgt
    15001 aatttatctt actccaaaag gtaaggtata caatcaatct atagcaaagc atatgtcttt
    15061 aaaagaagat ataatactgt tatgtggtca ttatgaagga atagatgaga gaatcattga
    15121 tttaatagtt actgatgaaa tttctattgg agattatgta ttaactggag gagagctccc
    15181 agcacttata atgatagatt ctatatcaag gttaatacca ggtgtattaa accaagaaga
    15241 atcatttgaa gaagaatcat ttaaagataa tttattggaa tatcctcatt acactagacc
    15301 tagagatttt gaaggattaa aagtaccaga agtgctttta tcagggaacc ataaaaagat
    15361 agatgaatgg agaagagaag aatctataag aataaccaaa gaaagacgat ttgatttata
    15421 taaaaagtct aatgaaaaat aaaaatataa taaatatgta tacaataaag tacctataaa
    15481 tggaattaaa aacataaaag tgcttgtaat aactattgaa gtatagtata atataaaatg
    15541 ttgaaaatac gggcgttcct ctgtttacac tggtcgggta gttaagaacg tctatgatat
    15601 ctaggaggaa gaaagatgaa tgaaatgcta agagcaattg agcaagaaca attaaaaaat
    15661 gaagttccta attttggacc tggggacaca gttaaagtac acgttagaat tatagaagga
    15721 aaaagagaaa gaatacaagt attcgaaggt gtagtattaa agagacaagg tggaggagca
    15781 agagaaactt tcacagttag aaaaatgtct ttcaatgttg gtgtagaaag aactttccca
    15841 gttcactctc caaaaataga aaaaatagaa gttactagaa aaggtaaagt aagaagagct
    15901 aaactaaact acttaagagg tagagtaggt aaagctgcta agataaaaga agctagaaat
    15961 aaataattaa aaactttact tataaatggg gggctgattt atcagtcctc tttctatttt
    16021 aatgaaaatt acagcaaata attatactaa aaataaatag aaatatttgt tgaaaatata
    16081 cattatttat ctatgaattt ataactttta aaatgaaaaa taaaattggt aaatattgca
    16141 ttgtattttg taacaatatt aaaaataaga gtacagtatt ttgtaaagta tgacgttaaa
    16201 aagatgacaa tattatagtt tgtgaagata tattttttta gtgaaattag gatataataa
    16261 tataatattt aataaggagt gatacatatg tcaaatgaat atgaagagta tcttatggat
    16321 gacaatctac atataaactg gtatccaggt catatgaaaa aaactaagga gttagtaaaa
    16381 aacaacttaa aattgattga tgtagtaatt gaacttttgg atgctagaat accgtttagt
    16441 agtaaaaatc cagatataga caggcttgta ggagataagc caagagttgt tgtacttaat
    16501 aagagtgaca tggcagataa ggataaattg aatcaatgga ttgaatacta taaaaaaatt
    16561 aatataaaag ctatacctgt agatacaata aaaggtgttg gtataaataa gataatcgaa
    16621 gaatgtaaga atgtaactag agaaaaaatg agttctttaa aagataaagg tagaaaagaa
    16681 agagctatac gaataatgat agttggggtt ccaaatgttg gtaaatcttc tcttataaac
    16741 aaattaacag gtagaaaaag tacgcaaact ggagataaac caggagtaac aaaagggaaa
    16801 caatgggtta gattaaaggg aaatcttgaa cttttagata cacctggaat actttggcct
    16861 aagtttgaag accaagaagt tgcattaaat ttagcattta gcagagctat aaaggatgag
    16921 attttagata ctgagacttt ggctttaaaa cttatagaaa aacttatgaa aatagaacct
    16981 gagaagttga aagccagata taagctagac tgtctaggag aaactccaat tgaaactatg
    17041 gatatgattg gtcataaaag agggtttata acaggtaaaa aagaactaga ttatacacgt
    17101 atagccacaa ctgtattgaa tgaatttaga gacgggaaaa taggaaatat aacattggaa
    17161 attccagaaa acgttaaaag ataaatttta aaacaaaatc aaaaatttaa tgttaaaaat
    17221 ttaaaaaagc ccttccagaa aggctttttt atttttttga aatatagaca agtatttctg
    17281 tataaaagta taaattaggt tagaaaacct ttaaaaatat taatttaaga caaatatatc
    17341 taatattttg aaaattaaaa tgagtgtgat tatttaaaat cttggcatat tgacttgctt
    17401 gtttcgtata atatataata agcacaaata aatacaatat attaaaaatt taatttattt
    17461 aattttatta atttaataat gagttcttat tgtttaattt aacttattta tgcaaacgta
    17521 aacaatcgag atattttatt aatttaataa ggttaaaaag ggggatttta taatgggtaa
    17581 gaccaaggat atcatagtat ttggatttgc gttatttgca atgttctttg gagcaggtaa
    17641 cttaatattt ccaccttact tgggaataat tacaggtcca gaatggttaa ttgccttctt
    17701 aggatttaca tttgctgatg ctggattggc acttctagca gtaatggcta cagctaaatt
    17761 cgatggaaac gtagttgaaa tgtttaaaag atgtggtatg aaattaggga ttcttatagg
    17821 ttgtgcagat attttatgta taggaccatt cttagctata ccaagaactg gtgcaacaac
    17881 ttatgaaatg ggtataatgc ctttatttgg aacttcaatt ccagtgttat tattctgtat
    17941 actttttttt gcgatttcat atgtactaac aataaaacct tctaaggtcg tagatatagt
    18001 tggacagttt ttaactccag ctcttctaat agctttagca tttataatta taaaaggtat
    18061 tatatcacct cttggagata tagtagataa acctatgata cctaatgtat ttgcagaagg
    18121 tataggtcaa ggatatcaga caatggatgc ttttgcagca atagcacttg catcagtact
    18181 aatagtatct ttaaatgata aaggatattc tactattagt gacaagttaa aaatgattgg
    18241 taaagctggt gttcttgctt gtggaggatt agctctagtt tatggaggat tatgtttcct
    18301 tggggcaact gtatcaacaa tgtatggaac agatgcagta caatcacaag taatagtaaa
    18361 tataactgaa gggttacttg gaaatgttgg gaaagtgatt ttagcagtag tagtttcatt
    18421 agcatgtttg acaacatcta taggtcttac atctgctaca ggtcaatatt tctctagact
    18481 cacaaaagga aagttgagtt atgaaaaaat agtattagct gtatctgtat tcagtgcagt
    18541 agtagcatct tttggtgttg gaactataat aaagatagca tctcctatac taagtatagt
    18601 ttatccacca tcaattgttt taataatttt agctttcttc aatgaaaaaa ttaagaatga
    18661 taatgtgtac aaaggagctg tatatatgtc attgcttgta agtatactaa cagttatatc
    18721 tagttatgga gtagctgttc cagtagtaaa ttctttacct cttcattcat taggatttaa
    18781 ctgggtggtg ccagttataa tagctggaat aattggtaat tttataccat caaagagtca
    18841 atctaacaca cttggaacaa actaagtttt gtttaattaa aatctaaaag taaaaggagt
    18901 tatttgtata aataactcct tttactttta ga
//
LOCUS       DAFNNB010000066        18814 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0067, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000066 DAFNNB010000000
VERSION     DAFNNB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18814
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0067"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..723
                     /locus_tag="L3M60_001973"
     CDS             <1..723
                     /locus_tag="L3M60_001973"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBN6226638.1"
                     /translation="ASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIM
                     QGYGTMAQEAIEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEAD
                     EADCYYKSAEAGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNHATAFVSAPDWVS
                     AKGMRTLGNPLNGDEKVISGESGAVTTGLLVAAMEREDLADLRKDLKLDENSRILLIS
                     TEGDTDPDKYRSIVWDGEYPSI"
     gene            849..2036
                     /locus_tag="L3M60_001974"
     CDS             849..2036
                     /locus_tag="L3M60_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBN6226639.1"
                     /translation="MLNEQRKQEVTEICQKLIQNPSSSGNEEGVVKAIEESFEKLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEGKIVDGKIYGRG
                     TSDMKGQYTAMMSAVAYFAKDTNKDFAGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGVNAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIKTIGFGPSKENLAHTIDEYIEQEQLFIGTEGYYGILKSV
                     YGK"
     gene            2169..3539
                     /gene="hydA"
                     /locus_tag="L3M60_001975"
     CDS             2169..3539
                     /gene="hydA"
                     /locus_tag="L3M60_001975"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBN6226640.1"
                     /translation="MGTILRGGTIITSDKTYISDLRIEDENIVEIGNNLEINGDKVID
                     ATGRIVIPGGIDTHTHFDMNAGSITTADNFETGTRAAIAGGTTTILDFAEANEGENLL
                     QGVEAYHKKAIGNCYCDYGFHMTITCLDKDTFKHMEKLISDGIVSFKMYMAYEGMKVD
                     DGTIYRVLKKARDLGCIVGFHCENGDLLNVLIEENIQNGNLEPKYHPLTRPNIVEKES
                     VSRLADITKLSNSKSYVVHLSCKESLKTVKMAREKNVDMIVETCPQYLLLEDNLYNKD
                     KFEGAKYVMSPPLRKKEDINCLWEGLASGDIQTVGTDHCSFNFKGQKDLGIDDFSKIP
                     NGAPGVEHRLALLYTYGVLENRISANKFVEVTSTNAAKIFGMYPKKGEIAVGSDADIV
                     ILNINKEETISYKTQKQNVDYTPYEGFKIKCKVEDVFLRGNHVVQSCNVKEQPIGQYI
                     KRKITK"
     gene            3557..4963
                     /locus_tag="L3M60_001976"
     CDS             3557..4963
                     /locus_tag="L3M60_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226641.1"
                     /translation="MMDILIKNAIIVTVNKEREVIFDGALVVKDNKIADIGNSKEIES
                     KYTDVKKIIDAKGKVLFPGFINTHTHLFQTLLKGLGDDMVLKDWLETMTFPAANYLEP
                     KDTYDAAMLGCIEGLRSGITTMVDYMYPHSKPGLCDGIIDAYKELGIRGILGRGCMNT
                     GAQFGVHPGIMQDVETVEKDVRRLFEKHHNTENGRIKIGVAPAAIWSNSQEMLEMLWR
                     VVKEYDDALFTVHISETPFDREAAKELHGQYDIDVLEKLGILGPNVLMVHCVYLTERD
                     MELAKKYDMKVSHNTASNMYLSSGVAPVPEMLKKGITVSLGVDGAASNNSQDMLELMK
                     LTALQHKVNKCDPLAMSAEKVLELATIDGARAIGMEDEIGSLEIGKKADLLIFNPMLS
                     PKAIPMHNPVSTLVYSSSMKNIESVIVDGNIIMEDSKILTTDEDKALKEAQDTAERLC
                     VRGTIKNRMEGHKWNSLY"
     gene            5036..5482
                     /locus_tag="L3M60_001977"
     CDS             5036..5482
                     /locus_tag="L3M60_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBN6226642.1"
                     /translation="MIIKCTINNKEKELSIRPDDFLADTLRENGYVSVKKGCDTGSCG
                     LCTVMVNDKAVLSCNYLTARVDGQHITTLEGVLDEAKEFGDFLADEGADQCGFCAPGF
                     IMMVLAMKRELQNPTEEEVIDYVNGNLCRCTGYKGQLRAIHKYLNT"
     gene            5521..6315
                     /locus_tag="L3M60_001978"
     CDS             5521..6315
                     /locus_tag="L3M60_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBN6226643.1"
                     /translation="MITIKEYVVPKSLDEAYELLISRKNNIILGGCGFLKLGSKNIGS
                     AIDLKNLALDYINETDDSILIGADTSLRTLELNKVIKNYCNGVISNAVSNIVGVQFRS
                     GARVGASVFAKYGFSDLIPSLLVVDAKVKLYKKGVMSLADFLESELEKDILIEVILPK
                     KDAIGVFDSIRKCTGDFAVLNGAMLKEGSIYKIAIGARPRRAKIAYKASEILSLENDI
                     EKAGEVSSEELSFGSNIRGSKEYRKDMAKALVLRMYNSIGGECDGK"
     gene            6305..8596
                     /locus_tag="L3M60_001979"
     CDS             6305..8596
                     /locus_tag="L3M60_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBN6226644.1"
                     /translation="MVNKEVRKIDSEAIVTGKPIYTEDLIFHKDVLTIKLLRSPHAFA
                     KIKNIDIKNALKVKGVVNIFTYKDVPSYRYSMVGESYPEASPHDQLILEDVVRYVGDE
                     VAIIAAEDERAAIKAMKLIKVEYEVMTPILDARESEGNKILIHEEDDLFCPFDFGLDA
                     TKNIVSREKMGKGDVEKEFKESDVIVEHSYMTQSQAHCMMETHRAYTYIDTNGRLVVT
                     SANQSAYHMRRQVSRALGIPLSKVRVIKPRIGGGFGGKNIAVTEIYASFVTWMTKRPA
                     KLIYTREETFAMTNTRHQMFFDIKIGADKEGNIKAIDMKALNNTGAYGDNGPSVCSES
                     GHNVLPTYNNVPAIKFDGKTVYTNLVPGGALRGYGATQGTFALDCAIDELAHKLNMDP
                     TEIKLKNIIKKQTEGGIMNFPIRSCALDECIIRGKKLIGWDEKYPVKDIGNNKVRAVG
                     MSVGTHGSGIANIDMAIVNMRMDEDGTYKLFSGSSDLGTGSDTILAQIAAKALNTSID
                     RISVYTGDTDMCPYDTGAYASCTTYVTGNATIKSAESLKRKILKVAQKKLDLPIDDLI
                     LYEDRVCYSEDVEKFVLLSELGKESVGGGNQEVLYSSESYGIQESAKPFLAGFAEIEL
                     DKTTGEFKVINYACSTDCGTVMNPALARIQVEGGVTQGIGLAMYEDPRHGSDGKLQTS
                     NLLQYKVPTKKDIGNIIVDFVDNYEPTGPYGAKSLGEIVIHTPAPAIANAIYNATGVR
                     IRELPITSEKVYMAMKKLNQGGK"
     gene            8597..9910
                     /locus_tag="L3M60_001980"
     CDS             8597..9910
                     /locus_tag="L3M60_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBN6226645.1"
                     /translation="MIAIKSKYLISSADEIYTNYAVVVENDIIKDILPNEEVEIKYKN
                     IEEVIDKSDAIIMPGFVNGHMHQYGVLSRGIPANVHFTDFEGFLNDYWWPFIENRIGL
                     KEVKATTKASAIELIESGVVAFCDTLEAPNTEEGTLIEQGKILEEIGMKAVLSLESCE
                     RISFENGLRCLDENSNLIKWSRENSKLINGIMCTHTSFTCSDKFIKKAKEDAKKLNAP
                     MQFHLCESIYEPNYAEKHFGKKAVDYYNDLDILDETVLASQCVKVNDEEIDILKEKGV
                     KVVHMPLSNCEVGGGFSPVPKMIKKGIKVALGTDGYINDFFEVMRGAFLMHKSVEEDA
                     SVMPANLVFRMATEHGAHVLGLQNSGKIAVGNKADIIVMEDEFKTPVTLDNIFDQIVV
                     QGKKEFISNVYIDGRHILKEKQLVDLDKKAIVKEMKEVACEFWNF"
     gene            9946..11031
                     /locus_tag="L3M60_001981"
     CDS             9946..11031
                     /locus_tag="L3M60_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBN6226646.1"
                     /translation="MYNFLGKELKSPLIIGSGPLTYSAAGCKILSDAGAGAVVTKTIR
                     KERAINPAPHMVRNTANALLNNEKWTDFEPEQWIDFEIPQMKRDGTVCIASVGHTIEE
                     SSELVEKVANAGADFIELVSYDYRDLIPMLKDAKERVNIPVIVKLPPMIDEIGDFAKK
                     LEEAGADAITACDSVGPAFRIDIETGQPLLGGNGIGYLSGETIKPITLQRIYEIKKQV
                     NIPIIGLGGCVSGDDALEMIMAGADFVGICSVVILKGAQVISKIHDDLKSNLNRLGYN
                     TIENACGIVHNHMGDVNERLSFEFTYDEEKCTKCKMCERVCAYQARKLNDKMELNEEE
                     CRYCGLCISVCKPKALGRKISCSELNV"
     gene            11261..12310
                     /locus_tag="L3M60_001982"
     CDS             11261..12310
                     /locus_tag="L3M60_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226647.1"
                     /translation="MVMQVQEKILELLYTINEDVVSIKNDMSEVKQEINVLKEDMSEV
                     RQEMTVMKEDMSEVKQEINIMKEDVSEVRQEINILKEDMLEVKQEINIMKEDMSEVKQ
                     EINIMKEDMSEVRQEMTVMKEDISEVKQEINVLKEDMSEVRQEMTAMKEDMSEVKQEI
                     NVLKEDMSEVRQEMAVMKEDMSEVKQEINVLKEDMSEVRQEMTVMKEDMSEVKLEVTV
                     MKEDVSEVKSEVNFMQNKINNINKDMSGIKEEVSIANEKLDGIEIKIDSLESEDKSMK
                     EMQVEQNNILGSLLHNSEINKATHDNIEHNIAYIKGDTNSIKEDIAEIRRDLNLVELA
                     TSKNWSDIVKLKSVK"
     gene            13644..15002
                     /locus_tag="L3M60_001983"
     CDS             13644..15002
                     /locus_tag="L3M60_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBN6226648.1"
                     /translation="MKAKSTSKFELDGMPPLKEAIPLGLQHLMAMVIGSCLPAILIAN
                     AAGLNHKMSTLLIQAALIFAGLSTLLQLYPIPLFKGFKLGGGIPVIMGATAMFIGAGV
                     SVAGKYGLPVLFGSQIIAGIVIIIIGFGLKKIRFIFTPAVSGTIVACMGLGLFPIAIK
                     NLAGGMGNETFGNQINFIVGISVSLMILGLNKYGKGLFKDASILVSIIFGYILSLILG
                     IVNFSSIQEFTLVALPKPLIFGLDIRLEVVVMFSIIYLVEIADIMGACTLSAVGGLNR
                     QVTDEELSSAVLGNGVLSTIGALFSATPMGMFGQNSAIVSNTKIVSKFVLAIGGIGLF
                     LAGISPLLANLIRTIPPCVVGGATLVIFSTLTTSGLRLVSMDGFNQENSMILGLSMAS
                     GIGFMVAPQVLERFPKFVETLLADSSVVSGAMVAIIIQALYMVKFPKNKNGKVEDKNK
                     SL"
     gene            15109..15903
                     /locus_tag="L3M60_001984"
     CDS             15109..15903
                     /locus_tag="L3M60_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBN6226649.1"
                     /translation="MVTIKEYSVPQSLEEAYKILTSKKNNVILGGCGFIKLSNKNIGT
                     AIDLKDINLNYIKEDEKNILIGADTSLRSLEMDKTIKNYCSGILSSAVSNIVGVQFRQ
                     GARIGASVFSKYGFSDLIPALLVVGAKVRLYNKGMLELNEFLDSELSRDILVEVILPK
                     KDAIAVFDSIRKCTGDFSILNSAMLKENNTYKIAIGARPQKARLALEASNILNKEKDI
                     DKASIVASKELTYGSNMRASKEYRKDMASALVKRMYNAIEEGMYND"
     gene            15896..18202
                     /locus_tag="L3M60_001985"
     CDS             15896..18202
                     /locus_tag="L3M60_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBN6226650.1"
                     /translation="MINKGIRKIDSTAIVTGKPLYTEDLIMHKDVLTLKLLRSPHAHA
                     KIKNIDTSKAEKIPGVVGIYTYKDVPQVRYCVPGQTEPEASPYDRVLLEDRVRFVGDE
                     VALVAAEDEKIALKALKMIKVDYEVLKPVFDAREAEGNEVVIHPEKDIVEPYPFGLDA
                     NNNIVSKEFFEFGDVEAKLKESDLVVESSYKTQSQSHCMMETHRSYSYKDINDRIVVV
                     SSLQSPHNVKRILRKVLGIPSSKIRVIKPRIGGGFGGKNIAATDIFTAFVTMKTGRPA
                     KFVFTRSETFVGSNSRHEMFFDVKVGADKEGNLKAIELKSLNNTGAYGGNGVSVSSES
                     GHNTLPIYNDIDAVRFNARTVYTNRLPAGALRGYGATQGIFALDSAIDELAHKIGMDP
                     LEFRIKNIIDRYTSGKAAEGEILSCELKQCIEKGKKLINWDDKYPCKDIGNNKVRAVG
                     MAVANHGSGIPRVDMATVTIRMDEDGTYKLLSGLADLGQGADTIQAQIAAEALNTTVD
                     RISIYTGDSDLCPYDTGAFASCTTTVTGNATIKTANKLKDILLSVAESKLGFKKEDLE
                     LGKDRVFVKNDNSKFVLLEELGRESVGGIGFASDDVPVVLEATESFGILKKIKPFIAG
                     FAEIELDKNTGEYKVINFACVPDCGTVINPTLARIQVEGGVMMGIGFAMYEDPRFDKD
                     GRLLTNSFLQYKVPTKKEVGNILVDFAENFDSGGPYGAKSLGEIVIHTPAPAIANAIF
                     NATGVRIRELPMTFEKVYMGMKNNNKQL"
     gene            18305..>18814
                     /locus_tag="L3M60_001986"
     CDS             18305..>18814
                     /locus_tag="L3M60_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN6226651.1"
                     /translation="MSSRVYSKGGELMSNRIKNIHYGTQRQQLKNLTKKEYQALREMC
                     FLAKNLYNVGTYNVRQHYFNQGEHLNYEANYHVCKENENYKLLNSNIAQQILKEVDSS
                     FRSFFGLIKLAKEGKYDFRGIKSPKYLDKEGFFSIIIGQIRIKEDGTLNVPMSPVFKK
                     LYGKVSIKVP"
BASE COUNT         7421 a         2027 c         3764 g         5602 t
ORIGIN      
        1 gctagtataa cagacttaaa ctatgatgat gcagtaagat tagctaatga ttatgcagaa
       61 gctgatgacc atggagtaat ggttcaagat acagcatggg acggatatga agaaatacca
      121 gcatggataa tgcaaggata tggaactatg gctcaagaag ctatagaaca attaaaagaa
      181 tatggagtag atagaccaac acacgtattt gttcaagcag gagttggttc acttgctggt
      241 gcagttcaag ggtatgttgc ttcaatatat gatgaatgcc caataacagt agtagtagag
      301 gctgatgagg ctgattgtta ctataaatca gcagaagctg gagatggaaa accaagattt
      361 gttggaggag atatgccaac tataatggca gggcttgctt gtggagagcc aaatactata
      421 ggatttgaag tgttaaaaaa tcatgcaaca gcatttgttt cagcaccaga ttgggtatct
      481 gctaaaggta tgagaacatt aggtaatcct ttaaatggtg atgaaaaggt aatttctgga
      541 gaatctggag cagttacaac tggtctttta gtagcagcta tggaaagaga agatttagca
      601 gatttaagaa aagatttaaa attagatgaa aattcaagaa tacttttaat aagcacagaa
      661 ggagatactg accctgacaa atatagaagt atagtttggg atggagaata tccaagtata
      721 taaggaatta ctaaaatgca attattagat attggtttac atatttagaa gatatataag
      781 ttctaaattt tagaaaatat atagtatata aaatacatac attataaaaa atctaggggg
      841 ataatgaaat gttaaacgaa caaagaaaac aagaagtaac tgaaatatgc caaaaattaa
      901 ttcaaaatcc tagttcttct ggaaatgaag aaggtgttgt aaaagcaata gaagagtctt
      961 ttgaaaaatt aggttttgat agctggtcaa gagatagata tggaaatata gtaggatgta
     1021 taaaaggtaa caagccaggt aagaaaatat tatttgatgg acatatagat acagttccag
     1081 ttccagatgc aagtaaatgg agtgttcctc cttttgaagg aaaaatagtt gatggaaaga
     1141 tatatggtag aggaacttct gatatgaaag gtcaatatac tgcaatgatg tctgctgtag
     1201 cttattttgc taaagatact aataaagatt ttgctggaga actatatgta gcaggtgttg
     1261 ttcatgaaga aatattcgaa ggagtttcag ctagagagat aagtaaagct gttcaacctg
     1321 actatgttgt aataggtgaa tcttcagagt taaacttaaa gattggtcaa agaggtagag
     1381 gagaaatagt agtagagact tttggtaaac cagctcactc tgctaatcca gaaaaaggtg
     1441 taaatgcagt ttataaaatg gctaatgtaa tccagaagat acaacagtta gtacctccaa
     1501 ctcatccagt tttaggtgat ggaatactag ttttaacaga tataaaatct tctccatatc
     1561 caggagcttc agtagtgcca gattattgca aagctacttt tgacagaaga ttattagttg
     1621 gagaaacaag agaaggagta ttagctccaa tacaagcttt attagatgaa atgatgaaag
     1681 aagacccaga attaaatgca aaagtttctt atgcagttga aaaagctgac tgctatacag
     1741 gaaatactat agaaagtgaa agattcttcc caggttggtt atatgacgaa gaagatgagt
     1801 ttgttcaagc agcgtataag ggactaaaag aagctggaat agattcagaa ataactcaat
     1861 actcattctg tacaaatgga agtcactatg caggtgaggc tggaataaaa actataggat
     1921 ttggtccttc taaggaaaat cttgctcata caatagatga gtatatagag caagagcaat
     1981 tatttatagg aacagaaggt tactatggaa tattaaaatc tgtatacgga aaataatgtt
     2041 tagttcacaa taaattattt aaatatagtc tagaggtgct gaatatagca cctttagaaa
     2101 atacaatttt aaataaaatt ttacgatacc tagaaaatac tttaaagctt aaaagttagg
     2161 aggtatatat gggaacaata ctaagaggtg gaactataat tacctctgat aagacttata
     2221 taagtgattt aaggatagaa gatgaaaata tagttgaaat aggaaataac ctagaaataa
     2281 atggtgataa ggttattgat gctacaggta gaattgtaat tcctggtgga atagacacac
     2341 atactcattt tgatatgaat gcaggttcta ttacaactgc agataatttt gaaacaggaa
     2401 caagggcagc tatagctgga ggaactacta caatactaga ttttgcagaa gccaacgagg
     2461 gggaaaattt actacaaggt gtagaagcat atcataaaaa agcaattgga aattgttatt
     2521 gtgattatgg ttttcatatg acaattacat gtttagataa agatacattt aaacatatgg
     2581 aaaagcttat ttcagatgga atagtatctt ttaagatgta tatggcatat gagggcatga
     2641 aagtagatga tggaacaata tacagagttt taaagaaagc aagagattta ggctgtatag
     2701 ttggatttca ttgtgaaaat ggggatttac taaatgtatt gatagaagaa aatatacaaa
     2761 atggtaatct agagccaaaa tatcatccac ttacaagacc taacatagtt gagaaagaat
     2821 ctgtttcaag gcttgctgat ataactaaat tatctaattc aaaatcctat gtagtccact
     2881 taagttgtaa ggagtcactt aaaactgtaa aaatggctag agaaaaaaat gtagatatga
     2941 tagtagaaac atgcccacaa tacctattat tagaagacaa tttatataat aaggataaat
     3001 ttgagggtgc taagtatgta atgtctcctc ctttgagaaa aaaagaagac ataaattgcc
     3061 tttgggaagg gttagcgagt ggagatattc aaacagttgg cacagaccat tgttcattta
     3121 attttaaggg acaaaaagac ttgggaatag atgattttag taagatacca aatggagcac
     3181 ctggtgttga gcataggtta gcccttttat atacatatgg agtcttagag aatagaataa
     3241 gtgcaaataa atttgtggaa gttacttcta cgaacgccgc aaaaatattt ggaatgtatc
     3301 ctaaaaaagg cgagatagct gtaggaagtg atgcagatat agtcatttta aatattaata
     3361 aagaagaaac aattagttat aaaactcaaa aacaaaatgt agattatact ccatatgaag
     3421 ggtttaaaat taaatgtaaa gttgaagatg tatttttgag aggaaatcat gtagtgcaaa
     3481 gttgtaatgt taaagaacag ccaataggac aatatattaa aagaaaaatt actaaataat
     3541 gcggggggaa ataagaatga tggatatact tattaagaat gccataatag taactgtaaa
     3601 taaagagaga gaagttattt ttgatggggc attggtagta aaagataaca agattgctga
     3661 tattggaaat tctaaagaga tagaaagtaa atatacggat gtaaaaaaaa ttatagatgc
     3721 taaaggtaaa gttttattcc caggttttat aaatacacat actcatttat tccaaacttt
     3781 attaaaagga ttgggcgatg atatggtact taaagattgg ctagaaacta tgacatttcc
     3841 agcagctaac tatttagaac caaaagatac ttatgatgct gcaatgcttg gatgtattga
     3901 aggtcttaga agtggtataa caacaatggt tgactatatg tatcctcata gtaagccagg
     3961 tctttgtgat ggaataatag atgcttataa agaattagga ataagaggaa tacttggaag
     4021 aggatgtatg aatacaggag ctcaatttgg agttcatcct ggaataatgc aagatgtaga
     4081 aacggttgaa aaagatgtac gaagattgtt tgaaaaacac cataatacag aaaatggaag
     4141 aattaaaata ggagtagctc cggcggcaat atggtcaaat tctcaagaaa tgcttgagat
     4201 gctgtggaga gtagtaaaag aatatgatga tgctttattt acagtacata tatcagaaac
     4261 tccttttgat agagaagcag caaaagaatt gcatgggcaa tatgacatag atgttttaga
     4321 aaaattagga atacttggac caaatgtact tatggtacat tgtgtgtatc taactgaaag
     4381 agatatggaa cttgctaaaa aatatgacat gaaagtgtct cataatacag caagtaatat
     4441 gtacttatct tcaggtgttg caccagtgcc agaaatgctt aaaaaaggaa taacagttag
     4501 cttaggtgta gatggagctg ctagtaataa ctctcaagat atgcttgaac ttatgaagtt
     4561 aacagcactt caacataaag ttaataagtg tgacccatta gcaatgtcag cagaaaaagt
     4621 tcttgagttg gcaactatag atggagcaag agctattgga atggaagatg agataggttc
     4681 attggaaata ggtaaaaaag cagacttact aatattcaat ccaatgcttt caccaaaagc
     4741 tatacctatg cataatccag tatctacatt agtatattct tctagcatga aaaatataga
     4801 aagtgttata gtagatggaa atataataat ggaagattcg aaaatattga caactgatga
     4861 agataaagca ttaaaagagg ctcaagatac agctgagaga ctttgtgtaa gaggaactat
     4921 caagaataga atggaaggtc ataaatggaa tagcctttat taataaatgt aatttattca
     4981 taaaaaagta tagtaaaaac tttaacttaa aaatatgagt tttgaggtga tttatttgat
     5041 tatcaaatgt accataaata ataaagaaaa agaattaagc ataagaccag atgatttttt
     5101 agcagataca ttaagagaaa atggatatgt aagtgttaaa aaagggtgtg atacaggctc
     5161 atgtggctta tgtacagtga tggtaaatga caaggcagta ctttcatgta attatcttac
     5221 tgctagagta gatggtcagc atattacaac acttgagggt gtcttggatg aagcgaaaga
     5281 atttggagat tttcttgcag atgaaggggc tgaccaatgc ggattttgtg ctcctggatt
     5341 tataatgatg gtcttggcta tgaaaagaga gcttcaaaat ccaacagaag aagaagttat
     5401 agattatgta aatggtaacc tttgtagatg tacaggatat aaaggtcaat taagagcaat
     5461 acataaatat ttgaacactt agacttttaa gtgaaaatta agttgatatg gggagagagt
     5521 atgataacta taaaggaata tgtagttcca aaaagtttag atgaagctta tgaactgcta
     5581 atatcaagaa aaaataacat aatacttgga ggatgtggat ttttaaaact aggtagtaaa
     5641 aatattggtt cagcaataga tttaaaaaac ttagctttag attatataaa tgaaactgat
     5701 gatagtatat taattggagc agatacaagt ttaagaactt tagaattaaa taaggttatt
     5761 aaaaattact gtaatggggt aatttcaaac gctgtttcta atatagtagg agttcaattt
     5821 agaagtggag ctagagtagg agctagcgta tttgctaagt atggattttc agatttaata
     5881 ccttcattac tagtggtaga tgcaaaggtt aagttatata aaaaaggggt tatgagctta
     5941 gctgattttt tagaaagtga gcttgaaaaa gatatattaa tagaagttat attacctaaa
     6001 aaagatgcta taggtgtatt tgattctata agaaaatgta caggtgattt tgcagtatta
     6061 aatggagcaa tgttaaaaga aggaagtatt tataaaattg caataggtgc tagaccaaga
     6121 agagcaaaaa ttgcctataa agcaagtgaa atattaagct tagaaaatga tatagaaaaa
     6181 gctggcgaag tatcaagtga agaactttca tttggaagta atataagagg aagtaaagaa
     6241 tatagaaaag atatggcaaa agccttagta ttaagaatgt ataatagtat tggaggtgag
     6301 tgtgatggta aataaagagg taagaaaaat agatagtgaa gcaattgtta ctggaaagcc
     6361 aatatataca gaagatttaa tttttcataa agatgtttta acaattaagc ttcttagaag
     6421 tccacatgca tttgcgaaga taaaaaacat agatataaaa aatgcgttaa aggtaaaagg
     6481 tgtagtaaac atattcactt acaaagatgt tccaagttat agatattcaa tggttgggga
     6541 gtcatatcca gaagcatcac ctcatgacca attaatactt gaagacgtag ttagatatgt
     6601 aggagatgaa gttgctataa tagccgctga agatgaaagg gctgctatta aagctatgaa
     6661 acttataaaa gttgaatatg aagtaatgac tcctatattg gatgcaagag aatcagaagg
     6721 aaataaaatt ttaatccatg aagaagatga tttgttttgt ccatttgatt ttggattgga
     6781 tgctacaaaa aatatcgtat caagagaaaa aatgggtaaa ggggatgtag aaaaagagtt
     6841 taaagaaagt gatgtaatag tagaacattc ttatatgact caatcacaag cccattgtat
     6901 gatggaaact catagagctt atacatatat agatacgaat ggcagattag tagtaacttc
     6961 tgcaaatcaa tcagcttatc atatgagaag acaggtttct agagcattgg ggatacctct
     7021 ttcaaaggta agagttataa aaccaagaat aggtggaggt tttggtggaa aaaacatagc
     7081 tgtaacagag atatatgcat catttgtaac ttggatgacg aaaagaccag caaaactaat
     7141 ttatactaga gaagaaactt ttgcaatgac taatactaga catcagatgt tttttgatat
     7201 aaaaattggg gcagataaag aaggaaacat aaaagctata gatatgaaag cattgaacaa
     7261 tacaggtgct tatggtgata atggaccttc agtttgctct gaatcaggtc ataatgtact
     7321 tccaacatat aataatgtac ctgcaataaa atttgatggg aaaactgtat atactaattt
     7381 agttccaggg ggagcattaa gaggatatgg agcaacacaa ggtacatttg cacttgattg
     7441 tgcaattgat gaattagcgc ataaacttaa tatggaccca acagaaataa agttaaagaa
     7501 tattataaaa aaacaaactg aaggcggaat aatgaatttt ccaataagaa gttgtgcact
     7561 tgatgaatgt ataattagag gtaaaaaact tattggatgg gatgaaaaat accctgttaa
     7621 agatattgga aacaataagg tgagggctgt tggaatgtct gtgggaactc atggttctgg
     7681 tatagccaac atagatatgg caatagtaaa tatgaggatg gatgaagatg ggacttataa
     7741 gttgttttct gggtcttcag acttagggac tggttctgac acaatacttg cacaaatagc
     7801 tgcaaaagca ttgaacacat ctattgacag aatttctgtt tatacaggag atacagatat
     7861 gtgtccatat gatactgggg catatgcttc ttgtactact tatgtaactg gaaatgctac
     7921 aataaaatct gctgaaagtt taaaacgaaa gatattaaaa gtagcacaga agaaacttga
     7981 tttaccaata gatgatttaa tactatatga agatagagtt tgttatagtg aggatgtaga
     8041 gaagtttgtt ttgttaagtg aattaggaaa agaatctgtt ggtggtggaa atcaagaagt
     8101 tttatattca tctgaatctt atggtataca agagagtgct aaaccattct tagcaggatt
     8161 tgctgaaata gagttagata aaacaacagg ggagtttaaa gtaataaatt atgcttgttc
     8221 tacagattgt ggtacagtta tgaatcctgc acttgctaga atacaagttg aaggtggtgt
     8281 aactcaaggt atagggcttg caatgtatga agaccctaga catggttctg atggaaagct
     8341 tcagacttct aatttattac aatacaaagt tccaacaaaa aaagatatag gaaatataat
     8401 tgttgatttt gtagataatt atgagccaac aggtccatat ggagctaaat ctttaggaga
     8461 aatagttatt catacacctg caccagcaat agcaaatgca atatacaatg ccacaggagt
     8521 tcgtataaga gaacttccta taacttctga gaaagtttat atggccatga aaaaattaaa
     8581 tcagggaggt aaataaatga ttgctattaa aagtaaatac ttaatatctt ctgctgatga
     8641 aatatataca aattatgctg ttgtagtaga aaatgacatt ataaaagata tattgccaaa
     8701 tgaagaagtt gaaataaaat acaaaaatat tgaagaagta atagataaat cagatgctat
     8761 aataatgcca ggatttgtaa atggccatat gcatcaatat ggagttcttt ctagaggaat
     8821 accggcaaat gtacatttta ctgactttga aggtttctta aatgactatt ggtggccatt
     8881 tatagaaaat agaattggat tgaaagaagt aaaggcaaca actaaagcat cagcaataga
     8941 acttatagaa tctggtgtag tagctttttg tgatacccta gaagctccaa atacagaaga
     9001 aggaactcta attgaacaag gaaaaattct agaagagata ggcatgaaag cggttttatc
     9061 attagaaagt tgtgagagaa taagctttga aaatgggctt agatgtttag acgaaaattc
     9121 taatttaata aagtggagta gagaaaatag taaattaata aatggaataa tgtgtacaca
     9181 tacaagcttt acatgttcgg ataaatttat taagaaagct aaagaagatg caaagaaatt
     9241 aaatgcaccg atgcaatttc atttatgcga gagtatatat gaaccaaact atgctgaaaa
     9301 acattttggc aaaaaagcag ttgactacta taatgattta gatatattgg atgaaactgt
     9361 attagcatct cagtgtgtaa aagtaaatga tgaagaaata gatatattaa aagaaaaagg
     9421 cgtaaaagta gttcatatgc ctcttagcaa ctgtgaagta ggtggaggtt tttctccagt
     9481 accaaaaatg attaaaaaag gcataaaagt ggcactgggt actgacggat atataaatga
     9541 tttttttgaa gttatgagag gtgcattttt aatgcataaa tcagttgaag aagatgcatc
     9601 agtaatgcca gcaaatttag tatttagaat ggcaacagag catggtgctc atgtattagg
     9661 acttcaaaat tctggtaaaa tagcagttgg taataaggct gatataatag ttatggaaga
     9721 tgaatttaaa actcctgtga ctcttgataa tatatttgac cagatagtag ttcagggtaa
     9781 aaaagaattt atatcaaatg tatacataga tggtagacat attttaaaag aaaaacagct
     9841 agttgattta gataaaaaag ctattgttaa agaaatgaaa gaagttgctt gtgaattttg
     9901 gaatttttaa cgaatgtaaa tatatatgaa aaggggaagc agataatgta caatttttta
     9961 ggtaaagaat taaaatcacc acttataata ggttcagggc cattaacata tagtgcggct
    10021 ggatgcaaga tattaagtga tgcaggagca ggggcagttg ttactaagac tataagaaaa
    10081 gaaagagcta taaatccagc accacatatg gttagaaata cagctaatgc tcttttaaat
    10141 aatgagaaat ggacagattt tgagccagag caatggatag attttgaaat accacaaatg
    10201 aaaagagatg ggactgtatg tatagcttca gtaggtcata caattgaaga aagcagcgaa
    10261 ttagtagaaa aagttgcaaa tgcaggagca gactttattg aattagtatc ttatgattat
    10321 agagatttga taccaatgct taaagatgca aaggaaagag taaatatacc agtaatagtt
    10381 aaacttcctc ctatgataga tgagataggc gattttgcca aaaaattaga agaagcagga
    10441 gcagatgcaa taactgcttg tgattctgta ggaccagcat ttagaataga catagaaaca
    10501 gggcaacctt tattaggggg aaatggaata ggatatttaa gtggagaaac tatcaagcca
    10561 ataactttac agagaatata tgaaataaaa aaacaagtta atataccaat aattggtcta
    10621 ggtggatgtg taagtggaga tgatgcctta gaaatgatta tggcaggtgc tgactttgtt
    10681 ggtatatgtt cagtagtaat cttaaaaggt gcacaagtaa tatctaaaat acatgatgac
    10741 ttaaaatcta atcttaatag attaggatat aatactatag aaaatgcttg tggaatagta
    10801 cataatcata tgggagatgt taatgaaaga cttagctttg agtttactta tgatgaagaa
    10861 aaatgtacta aatgtaagat gtgtgagaga gtctgtgcat atcaagcgag aaaattgaat
    10921 gataaaatgg aattgaatga agaagagtgt agatattgtg gactttgtat atcagtttgt
    10981 aaaccaaaag cacttggaag aaaaatttca tgttcagagt tgaatgtata gattatttta
    11041 taaaaaataa agttttaaat aaaaataaga aggcctatct caaaatagag ataagccttt
    11101 tttgttgacc ttatttttgt ttatttatag ttattttaga tattaattag ttttgaagta
    11161 ataaagtgcg taaattttgt ttaattaaaa ttttaataat tcttatatat atgttaacat
    11221 tgtatagcat ttgtgtaaaa tgttataata attaataggg atggtgatgc aagtgcaaga
    11281 gaagatttta gaattattgt atactataaa tgaagatgta gttagcataa aaaatgatat
    11341 gtcagaagta aaacaggaaa taaatgtgtt aaaagaagat atgtcagaag taagacaaga
    11401 aatgactgta atgaaagaag atatgtcaga agtaaagcaa gaaataaata taatgaaaga
    11461 agatgtgtca gaagtaagac aagaaataaa tatattaaaa gaagatatgt tagaagtaaa
    11521 acaagagata aatataatga aagaagatat gtcagaagta aaacaagaga taaatataat
    11581 gaaagaagat atgtcagaag taagacaaga aatgactgta atgaaagaag atatatcaga
    11641 ggtaaaacaa gagataaatg tattaaaaga agatatgtca gaggtaagac aagaaatgac
    11701 tgcaatgaaa gaagatatgt cagaggtaaa acaagagata aatgtattaa aagaagatat
    11761 gtcagaggta agacaagaaa tggctgtaat gaaagaagat atgtcagagg taaaacaaga
    11821 gataaatgta ttaaaagaag atatgtcaga agtaagacaa gaaatgactg taatgaaaga
    11881 agatatgtca gaggtaaaac tagaagtaac tgtaatgaaa gaagatgttt ccgaagttaa
    11941 atcagaagta aactttatgc aaaataaaat aaataatatt aataaagata tgagtggaat
    12001 taaggaagaa gtatccatag caaatgaaaa attagatgga attgaaatta agattgatag
    12061 tctagaaagt gaagataagt caatgaaaga gatgcaggta gaacagaata atattttagg
    12121 aagcttatta cataattctg aaataaataa agctactcat gataatatag aacataacat
    12181 agcatatata aaaggagata ctaattctat aaaagaggat atagctgaaa taagaagaga
    12241 cttaaattta gtagagttag ccactagtaa aaattggagt gatattgtca aactaaaatc
    12301 agtaaagtag tattttaatt cactaattta tcatccacaa acataaaaga atttatagtg
    12361 ttcttactat ttattataaa ttttagaaat gaaaataaaa aattgagaaa agaccgttaa
    12421 aaatttattt ttaggtcttt tttttatgca aatataactg atttataaaa ataaaaccta
    12481 aaatttaaat gataaaatct aatagaacga ttgaaataag taaaatttta tcaataaaaa
    12541 cataaaaatt ttatactatg tgaatgaatg caaatattgg cactaaaatt gctctataaa
    12601 aattataaat agtaatatga ataaaatttt ttctataaga acaatattac tattttactt
    12661 atagatatat atatatctat aaatagatag aaacttgtat attttatgtt gcagatacaa
    12721 atatagtaaa ggagagtaca ttggtacaac tgagatacat agcttattaa gtttttctgt
    12781 cttatgagaa ttatattata aaatattcat aaatattttg agtttaaatt aaaaaattat
    12841 tttatgctta gattaattgc tatttaatta atttaataac aaaaacagtg aaagagtaaa
    12901 tagtattatt tacagggggt aaagcaaagt atattaaata aattgtactt ttagccccgt
    12961 acatagagaa atctatgtag gacacaactt taaaaccagt aaatcctaaa gccttacaac
    13021 tacaataaaa gatagaaaga aacttttatg aatgtggtga aaaccagaaa taatagtgag
    13081 gatggtatat ggtgagagcc taagtactta aaaatggaca tttggtcgcc aagcctagaa
    13141 ataggaaggt caaacgacta tctcgagaga gagtacacta taagtgattg atagtggaaa
    13201 cgggttgctc ttagcaggta atgctgaaga tgaagaaata gtctacgctc atatgaaagt
    13261 atgaggtcac tggatttgaa gtgacatcta taaagtagcg tttataggta aatgtttaaa
    13321 acttgaaaaa tataaatact tatgaaaact tataacttat aatgattaag attgaaagat
    13381 ttaaattgaa ttaaatttac tataaaaaat taaaaattca tagctataat actcttatat
    13441 ttgtaaagca ttatatataa tatatgcaaa attaaagaat aagtttattt gttaataatt
    13501 aaatttattg tatccttttt tagaccaaaa acttaaatat gtgttggagt atagttgcga
    13561 tttatgaaca tcattaattt aatatattag atttttaagt atttatataa ttcaataagt
    13621 tttatgtaac gggagttttt attatgaaag caaaaagtac atcaaaattt gaattagacg
    13681 gaatgccacc actaaaggaa gcaatacctt tagggttaca acacttaatg gcaatggtaa
    13741 taggtagttg tttaccagcg atattgattg caaatgcagc tggacttaat cataagatgt
    13801 caacattatt gattcaagcc gcattaattt ttgcaggatt atcaacttta ttacaattat
    13861 atccaatacc tttgtttaaa ggttttaaat taggaggagg aataccagtt ataatgggag
    13921 ctacagctat gtttatagga gctggggtat ctgttgcagg taagtatgga ctaccagttt
    13981 tatttggttc tcaaataata gctggtatag taataattat aattggtttt ggtcttaaaa
    14041 aaatacgatt tatatttact ccagctgtat ctggaacaat agttgcatgt atgggtcttg
    14101 ggctttttcc aatagctata aaaaacttag ctggaggtat gggaaatgaa acttttggga
    14161 atcaaattaa ttttatagtt ggaatatctg tatctcttat gatattagga ttaaacaagt
    14221 atggaaaagg attatttaaa gatgcatcaa tattagttag cataatcttt ggatacattt
    14281 tatcattaat tttaggaata gtaaattttt cttcaattca agagtttact ttggttgcac
    14341 ttccaaaacc gcttattttt ggtctagata taagacttga agttgttgta atgttttcaa
    14401 taatatattt agttgagata gctgatataa tgggtgcctg cacactttct gcggtaggag
    14461 ggcttaatag acaagtaaca gatgaagaac tttcttctgc tgttttagga aatggagtac
    14521 tctctacaat aggtgcatta ttttctgcaa ctccaatggg gatgtttgga caaaattctg
    14581 caatcgtttc caatacaaag attgtaagta aattcgtact agctattgga ggaattggac
    14641 tatttttggc gggaatatcg cctttattag ctaatttaat aagaacaatt cctccttgtg
    14701 tggttggagg ggcaacactt gttatatttt caactcttac aacatctggt ttacgacttg
    14761 ttagtatgga tggatttaat caagaaaata gcatgatatt aggtctttct atggcttctg
    14821 gtattgggtt catggtagct cctcaagttc ttgaaagatt tccaaagttt gtagaaacat
    14881 tacttgcaga ttcaagtgta gtttcaggag ctatggtagc cataataatc caagctttat
    14941 atatggtaaa atttcctaaa aataaaaatg gtaaagttga ggataaaaat aaaagtttat
    15001 aatgtaaaga ttttatacat gtgaattaaa tatactacaa ttataaatat aaataaaaag
    15061 atttattaaa actcttatgt tttaataagc taatgatggg gggataagat ggttactata
    15121 aaggaatatt cagttccaca gagtttagaa gaagcatata aaattttaac ttctaaaaaa
    15181 aataatgtta ttttaggtgg atgtggattc ataaaattaa gcaataaaaa tataggaaca
    15241 gcgatagatt taaaagatat aaatctaaat tatataaaag aggatgaaaa aaatattttg
    15301 ataggtgcag atacttctct tagaagttta gaaatggata aaactataaa aaattattgt
    15361 agtggaatac tttcaagtgc tgtttctaac atagttggtg ttcaatttag acaaggagct
    15421 agaattggtg caagtgtatt ttcaaagtat ggtttttctg atttaatacc agcacttttg
    15481 gttgtcgggg caaaggtaag attatacaat aaaggaatgc tggagttgaa tgagtttttg
    15541 gatagtgaat taagtaggga tatattggtc gaagtaatac taccgaaaaa agatgcgata
    15601 gctgtttttg actcaataag aaagtgcaca ggagattttt ctatattaaa ttcagcaatg
    15661 ttaaaagaaa ataatactta caaaatagca attggggcaa gacctcaaaa agctagatta
    15721 gcacttgaag caagtaatat attaaataaa gaaaaagata tagataaggc atctattgtt
    15781 gctagtaagg aacttactta tggaagtaat atgagagcta gcaaagagta cagaaaggat
    15841 atggcaagtg cattagttaa aaggatgtat aatgctattg aggagggaat gtataatgat
    15901 taataagggg ataagaaaga tagatagtac agcaattgtc acaggaaaac ctctatatac
    15961 tgaagacctc attatgcata aagatgtttt aacattaaag ttacttagaa gtccacatgc
    16021 ccatgctaag ataaagaata tagatacatc aaaagctgaa aaaattccag gagttgtggg
    16081 tatttatacg tataaagatg taccacaggt cagatactgt gttccaggcc aaacagagcc
    16141 agaagcatca ccttatgata gagttttact tgaagataga gttagatttg ttggagatga
    16201 agtagcttta gtggccgctg aagatgagaa aatagcactc aaagccttaa aaatgataaa
    16261 ggtagactat gaggttttaa aacctgtgtt tgatgcaaga gaagcagaag gaaatgaagt
    16321 tgttatacat ccagaaaaag acatagtaga gccataccct tttggattgg atgcaaacaa
    16381 taatatagta tcaaaagaat tttttgaatt tggagatgtt gaggctaagc ttaaagaaag
    16441 tgatcttgtg gttgaatctt catataaaac tcaaagtcaa tcacattgta tgatggaaac
    16501 tcataggtca tattcatata aagatataaa tgatagaatt gttgtagttt catcattaca
    16561 atcaccacac aatgtaaaga gaatccttag aaaagtattg ggtataccat cttcaaaaat
    16621 aagagtaatt aaacctagaa taggtggtgg atttggagga aagaatatag cagctacaga
    16681 tatatttact gcgtttgtta ccatgaaaac tggtagacca gcaaaatttg tatttacaag
    16741 aagtgaaact tttgtaggtt ctaatagtag acatgaaatg ttttttgatg ttaaagtcgg
    16801 tgcagataaa gaaggaaatt taaaggcaat agaattaaaa tcattaaaca atacaggtgc
    16861 ttatggtggg aatggtgtct ctgtttcttc tgaatctgga cacaatacct taccaatata
    16921 taatgatatt gatgctgtta gattcaatgc tagaacagta tatactaata ggttaccagc
    16981 aggagcactt agaggatatg gtgctacaca gggaatattt gcattagata gtgctattga
    17041 cgaattggca cataagattg gaatggaccc acttgaattt agaattaaaa atataataga
    17101 taggtatact agtggaaaag cagctgaagg tgaaatatta agttgtgagt tgaaacaatg
    17161 tatagaaaag gggaagaaat taattaattg ggatgataaa tatccatgta aagatatagg
    17221 aaataacaaa gtaagagcag taggtatggc agtagcaaat catggttctg gtataccacg
    17281 tgtagatatg gcaacagtga ctataagaat ggatgaagat ggcacataca aattattatc
    17341 tggtcttgct gacttaggtc aaggagctga tacaattcaa gcacaaattg ctgctgaagc
    17401 tttaaatact actgtagata gaatttctat ttatacaggt gattcagatt tatgtccata
    17461 tgatacagga gcattcgctt cttgtacgac aacggttaca ggaaatgcaa caataaagac
    17521 agcaaataaa ttaaaagata tattactttc agttgcggag tcaaagctag gatttaaaaa
    17581 agaagattta gagttgggaa aagacagggt ttttgttaaa aacgataata gtaagtttgt
    17641 tttattggag gaattgggta gagaatctgt tggaggaatt gggtttgcta gtgatgatgt
    17701 accagtagtt ttggaagcaa cagaatcttt tggtatatta aaaaaaataa aaccatttat
    17761 agcaggattt gcagagatag aattagataa aaatacaggc gaatataagg taataaattt
    17821 tgcatgtgta cctgattgtg gaacagttat aaatccaact ttggctagaa tacaagtaga
    17881 aggcggtgtg atgatgggta tagggtttgc aatgtatgaa gaccctagat ttgataaaga
    17941 tgggagatta ttaacaaata gtttcctaca gtataaagtt cctacaaaaa aagaagttgg
    18001 aaatatattg gtagattttg ctgagaattt cgactcagga ggtccttacg gtgctaaatc
    18061 attaggagaa atagttatac atacaccagc accagctata gcaaatgcaa tatttaatgc
    18121 tacaggagtt cgtataagag agcttcctat gacttttgaa aaagtttata tgggtatgaa
    18181 aaataataat aaacaattat aataagtaat atgttgtata aaaacttgta aatttaaatt
    18241 attttgtaaa ataaagtaat taaatggaca ttatatatgg tgttttataa aaataccgta
    18301 tataatgtct agtagagtat attctaaagg aggtgagtta atgtcaaaca gaataaaaaa
    18361 tatacactat ggaacccaga gacaacaatt aaaaaactta actaaaaaag aatatcaagc
    18421 tctacgagag atgtgcttct tagccaagaa cttatataat gttggtacat ataatgtgag
    18481 acaacattat tttaatcaag gagaacatct taactatgaa gctaattacc atgtatgtaa
    18541 agaaaatgag aattataagc ttctcaatag taatatagcg caacaaatat taaaagaagt
    18601 cgacagttca tttaggtcgt tttttggatt aattaaatta gccaaagaag gtaaatatga
    18661 ttttagaggg ataaagtcac ctaaatattt agataaagaa ggatttttta gtataataat
    18721 aggtcaaata agaatcaaag aagatgggac tctaaatgta cctatgtctc cagtatttaa
    18781 aaaactatat ggaaaagtaa gtataaaagt tcct
//
LOCUS       DAFNNB010000067        18511 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0068, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000067 DAFNNB010000000
VERSION     DAFNNB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0068"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(126..1106)
                     /locus_tag="L3M60_001987"
     CDS             complement(126..1106)
                     /locus_tag="L3M60_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HBN6226652.1"
                     /translation="MIFDGHSDTWADVTIRRLNGEKDVFRRIHLDNFKRANIEGAIFV
                     IWIDPPYDKEPEKRVKQIISSIKDEIEDMKDVVNIVKKYSDIDKGISEHKINILIGLE
                     GLSHIGSNIDSIDYFYKEVGARHASLTWNEQNELATGCLGDENRGLTEIGKKAVHKME
                     NLGMIVDVSHLNEKSFWDLMSIASKPVIASHSNAKSLCDVKRNLTDNQLKEIANTGGL
                     VGINSFRGFTNSNPKKQDLIHLVDHIDYMVNLIGIEHVSFGFDFSDYLGKETIGSFTG
                     NSSMPSVDGLESTHTAHNIVVELENRGYKKRDIDLISSGNFYRLFKYILK"
     gene            complement(1163..2365)
                     /locus_tag="L3M60_001988"
     CDS             complement(1163..2365)
                     /locus_tag="L3M60_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 domain-containing protein"
                     /protein_id="HBN6226653.1"
                     /translation="MKSFVSPDNIWVLWAVVTGWAAFSIYLEQKYNWASKVSGAIIAL
                     VGAMLLSNLNIIPVESVVYDQVWGYVVPLAIALLLYQCNIKKIWKESGRLLIIFLVGS
                     VGTVLGAMIGFLALKNVVPDLNIVAAMMTGSYIGGNVNFAAMSGAFDAPGELVSATVV
                     ADNLLMALYFFVLIAIPSIGFFRKHFKHPHVDEMESIGVTEGETVAANYWGRKEISLK
                     DIAIAIGSAFIIVAVSVELSTWFKAIIPLSNPFLAMLNTLFGNQYLIITTLTMLFATF
                     MPNFFGDIKGAQELGTFLIYIFFVVVGVPASITLIIQKSPLLLLYCGIMVLINMLVTF
                     IVAKIFKFSLEEAILASNANIGGPTTAAAMAISKGWSKLVGPILIVGTFGYIVGNYFG
                     LLVGNILI"
     gene            2559..3113
                     /locus_tag="L3M60_001989"
     CDS             2559..3113
                     /locus_tag="L3M60_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBN6226654.1"
                     /translation="MNIDVGSKIRSLRKSKNISISTLAKNSDLSTGLISQIERNMVVP
                     SIVAMWKISKALDINIGYFFEEIGREDSDIVVKKNNRKKIVTNDSTKFYELLVPNLSG
                     KKIEFILVTLDGHTQKNSEFVTHEGEECGYIIKGKMKITLGNKEYLLEEGDSFYFNST
                     IPHVYENYDDDVCISVWAMTPPSF"
     gene            complement(3228..4019)
                     /locus_tag="L3M60_001990"
     CDS             complement(3228..4019)
                     /locus_tag="L3M60_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HBN6226655.1"
                     /translation="MIVFSDLDRSIIYSNKFLSADSKYANIEIYREKEISYISLDTIN
                     LIKQIQYYGMFIPTTTRTVEQFKRIEFNKYGIYFPWSITSNGGVILKDNEILKSWSEK
                     IDKLKSDYEPIESMIYKFKDYLNVDGITNFKVAEDTFFYIVVDFSKFNLESIKEYTNV
                     LESKNWKFYVSGRKIYFIPKEISKENAIKYLTKELGIEYFYAIGDSIMDYGMLNISNK
                     AYLLKHGDINKNEIENSFLSSSFNGMSGTEEILSNILDENCLLNI"
     gene            complement(4047..5135)
                     /locus_tag="L3M60_001991"
     CDS             complement(4047..5135)
                     /locus_tag="L3M60_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HBN6226656.1"
                     /translation="MRDFSTYSKDDVIFLLKDISNLIEEEGNKERELKIQNGRHYSEM
                     IPIEYEVSEDYLNLYHEKLRENKDKLAFSIGVMCEKIIKKNGNDVVLVSLARAGTPIG
                     ILAKRYVKSKYNINLPHYTISIIRDKGIDMNAIEYIVKNHPSSKIQFLDGWTGKGTIS
                     KELEKACDELNDKFKKHFDSTLAVLADPAGYSGLYGIREDFLIPSACLNSTVSGLVSR
                     TVLREDLIGKNDFHGAKFYRHLKDKDESMNYIETIEECFKNQFKNISDEVENWESDII
                     TRDGYFDVLNIKEKYNITDINFIKPGVGETTRVLLRRVPYKILVKDLNDKSLDHIFVL
                     AKEKNVEVEQMDLKAYKCCGIIKNMKDV"
     gene            complement(5189..6427)
                     /locus_tag="L3M60_001992"
     CDS             complement(5189..6427)
                     /locus_tag="L3M60_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HBN6226657.1"
                     /translation="MKNCLNFNCEIDIIDNPLNLELNDFLDIGIRNNSKRRFLFISKK
                     LGKHLACKPSEMDNLGKLIATIYNEKNKEESNGTVIAFAETGTAVGHSFFDYLCGDYE
                     FIHTTREQFDELENLEFLEEHSHATDQNLYFGMLNNFKFGDEIILVDDEITTANTCMN
                     IIRKIQSIYPKKKYTICSILNWVSDENLSKVTRLERELDCKIDFVYLFKGNFKFYIDE
                     DIISNYNDNYTITKLDTILSDKVNLVVNHIYVDLEDYIGNKKYVKYTGRFGINRREQF
                     RLKEIVKRESKKITIDNKESLKNEKEKILFLGTEEFMYIPMLFAKEKENQCDVYYHST
                     TRSPIINIDKHNYPIRSKFMLKSFYNMDIQNYIYNIDKQNFSKCYLFVELNKSENSYL
                     EFIDIIKKTSIKELTIVCCS"
     gene            complement(6405..7481)
                     /locus_tag="L3M60_001993"
     CDS             complement(6405..7481)
                     /locus_tag="L3M60_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HBN6226658.1"
                     /translation="MKYFDYICKDDLERIFLKEPEDFSAKTEKDVLKYALGAFLYVPA
                     TQYNMIYKSIIGDVKGVRPLAICLEDAVGVNGELEAIENLRLILKNISNESITNKDGI
                     PLIFVRIKDVEQLLRIKEIIIKNSHSITGILIPKANSELIENCIEALDSMNLQDMYVI
                     PIIETREFIYNEKKELSFINLYNAILRHKSRILSIRVGITDILGMYGIRRDKNFSIYN
                     NLICSSFILDIITYLQRPELDIPISGGVSEFFDMKNKDIRNKYIEEILLDKYHGLIGK
                     TVIHPMQIQIVQALSTVSYEDFTDALDILDSTDSKYGVSKGVLGERMNEINPHFLWAK
                     KTLILSKIYGVLNEGVDYEELLKF"
     gene            complement(7543..8679)
                     /locus_tag="L3M60_001994"
     CDS             complement(7543..8679)
                     /locus_tag="L3M60_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HBN6226659.1"
                     /translation="MGININSFSKDKNSVDVTENLPTAVQEENFDIMEYTNNKKNELR
                     KSKEVEALTSLIEVENPDTILQFGRKASEGVARVSDSLLNTIKLNRNEENSKMLVHLT
                     KIMDKFDLDDFQETKEPNFVQKLFKKANNAIEMMFQKYETLGGEVEKIQIELEQYERD
                     IALSNKQIGAMLNENFEFYNELQKYIVAGEMAIEEMDNEILPFFKQKSETSGDQMDVV
                     NYQELLKVYDMLNQRVYDLRIAENIAIQTIPMLRGMQHNNYGLIRKINSAFVVTLPVF
                     KQCLSQAILLKKQELQAKSLKALDDKTNELLLRNAQNVSSQSAQIARMAGTSSVQIET
                     LEKMYNTIKSGIDETMRIEENNRALIKDNTKRLEELNTTIIYNK"
     gene            complement(8680..10242)
                     /locus_tag="L3M60_001995"
     CDS             complement(8680..10242)
                     /locus_tag="L3M60_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceG family protein"
                     /protein_id="HBN6226660.1"
                     /translation="MGDSKVFEKIFSSQSDRGNYTPSKGYLSYFISYIGLEDEVLYNL
                     EIFKTKQNIDSKKDIALFTDVIANPSDFDIINYFKNGLQKYRTSMEDVDINILGFEEI
                     DYKIKQAMDKVLKEEEKEFTNDRVKQNFIVKIMAWIKIYIGALDINKNEAPKVIFYGD
                     IKKHEVYLLLILYLAGFDVLYLNPNSKSNIDILKSERYNVEFEEANIIEEKTSFEERV
                     ILGEKIDKSSVKKAFTVGAEASKRISEELLNDAGFIKPWQLQDRKIKNLLLSSTVDEI
                     SIYWNQPLKLRPGFNFNDAIVEAPNFLSKINGIYNDKNEYIKFLDLLRDSESSTFIEF
                     NGDVDRFSKAFTREAFSLSFLLDSKGVIDKNSVLNNKDYSISTLALNQQIMILEKVEE
                     LLEGSMFLNGLSGEDKIKGLFTVLHMDKKFVHMMNNFDYSLINPKLIIYMYKSIIFDK
                     EIVFLMLLLSKIGFDIIILCPGGENNIENVINNQLIDVHRLDKMVYDLKLNSLENDIP
                     LLKKIFGKRRRF"
     gene            complement(10329..13088)
                     /locus_tag="L3M60_001996"
     CDS             complement(10329..13088)
                     /locus_tag="L3M60_001996"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, PMCA-type"
                     /protein_id="HBN6226661.1"
                     /translation="MEFNNDIKYYNVDKEEVTKDLSVNPEKGLSESEVKTRREKYGLN
                     EFTPKEEGSFWDDLKESLSEPMIVILIIAAVVSAVIGETHDAIGIVGAIAIGIAIGMI
                     TEGRSKKAAEALSKLTENIEVKVLRDGEVHQISKSELVPGDIVYIETGDMVPADGRLI
                     ESINLKIREDMLTGESDDVSKKCDVVVSMEEIESKGAVVVQEPIPAKQINMVFGGTLV
                     AYGRGALVVTSIGDNSEMGKIAQNLSETDEQTPLQIKLGNLGGLIAKVSSAVAGLLFI
                     FMVFQMVSKNVLNVDMSGILPFLESIDPVKTAFTVCVALIVAAVPEGLPTMINMTLAI
                     TMQKMAKINALVTKKEACETIGSVSVICSDKTGTLTENRMTVEVAYVDGRYIESSEEE
                     LNSYFEENCMINSTADVEHNDGDIKYLGSATECALLLYYKNVDYRQARKNSNIVAQNP
                     FTSDSKRMTSVIGQDNHHVLLSKGAPEVLLELCSHIQRGKDIVPLTENIKHEILEEIK
                     KLQIKSMRTLGFAYKEISQAEEEAAVTAESDAMNVSAMENNLVFSGFVGIRDPLRKDV
                     IESVNTANKAGVSVKMLTGDNINTARAIGEELGLLKNNMRVVEASYIDTLDDEELKEE
                     IQSISIVARSKPDSKMRIVSALQKSGDVVAVTGDGINDAPALSKADVGIAMGISGTEV
                     SKNAADIILTDDSFSTIVKGIKWGRGIYDNFQRFVQFQLTVNVIAFLVAIISQVMGQE
                     MPFTTIQLLWVNIIMDGPPALALGLEPVRDHVLNRKPVDRKANIISKSMVYTIVLNAF
                     YITAILMLQSTFNFLGVDEAHKGTVMFALFAFSALFNAFNCREFNADSIFPNFTHNKL
                     ALQIIVLTGIAQVIFTQLFQDFFNSVAMDFMTWVKVLCVAASVIVVNEVVKCIIRLVK
                     PKKTA"
     gene            complement(13078..13707)
                     /locus_tag="L3M60_001997"
     CDS             complement(13078..13707)
                     /locus_tag="L3M60_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN6226662.1"
                     /translation="MSINLSKGDKIDLKKSNPGLSNILVGLGWDPVQQSGGGFFKSLF
                     GGGQADIDCDASVFMLNQEGKLSGIKDLIYFGNLKSACKSVLHTGDNLTGEGAGDDEQ
                     ILVNLDKVPSNIHKLLFVVNIYNCVDRKQHFGMIENAYIRVEDQGNKKEIAKYNLSDN
                     YSEKTTLIVGAIYRKDGSWQFKAIGEGTKDAGLKEVMQNLDRIECAYGI"
     gene            complement(13793..14371)
                     /locus_tag="L3M60_001998"
     CDS             complement(13793..14371)
                     /locus_tag="L3M60_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN6226663.1"
                     /translation="MAIVLKKGQKIDLTKGNPGLKNIKLGLGWDTNSFDSGYDYDLDV
                     SVFMVGESQRVEKDEDFIFYNNLKHPSGAVEHLGDNRTGEGDGDDEEILVNLEAMPKH
                     IQKIAVTVTIYEAAERRQNFGQVSNSYIRVLNSENDEEILRYDLGEEFSIETAITVCE
                     IYKYNGEWKFSAVGAGFEGGLEALCRNYGLNV"
     gene            complement(14410..14988)
                     /locus_tag="L3M60_001999"
     CDS             complement(14410..14988)
                     /locus_tag="L3M60_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_126936537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN6226664.1"
                     /translation="MGITLSKGQKVDLTKGNPGLKNILVGLGWDTNKYDGGFDFDLDT
                     AAFLTGASGSVTNDGDFVFYNNLKHTSGAVEHLGDNRTGEGDGDDEQIVVDLSKIPGE
                     ISKISFTVTIHDATERRQNFGQVSNSYIRIVDKDTNEELIKYELGEDFSIETAIVVAE
                     IYKHNGEWKFNALGSGFEGGLAALCGNFGINL"
     gene            15317..16753
                     /locus_tag="L3M60_002000"
     CDS             15317..16753
                     /locus_tag="L3M60_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HBN6226665.1"
                     /translation="MICTIKPLKISIKKSNEPLIEFMSNTTYQNKSSSYIKEVYFTRY
                     IGVDDSIDKYIEKIKNIDTYFFNNPIIPYIRLSSLNINFDKEQTDKMLKIFNIYDSAK
                     FNSWDLYNINFPCKIENDTLNWTKKIAFKNTLDLFSMSMPNCTPSIIKNFAVKLLCWI
                     EYFLPKLFYNKVNTNISPKILYFGNIKRQELFFLYFLSQLGCDILYINPNKDILDLYP
                     ECSKFSNIFELSRKTPNILEIPFERHIANINYNENTNKTSHPMNKPNSENTFNNTNLI
                     SKKIVQGEEVELSYEELAKLSASVVMIVVCNKENKPFKSGSGVVINNEGYILTNLHVV
                     NDGYSFLVRFENDDKVYTSYQIIKYHSGYDLAVIKVDRKCKPIPVKIGKKPVRGQKIV
                     AIGSPLGLFNTVSDGIISAFRDFETVQMIQFTAPISSGSSGGALLDMFGNLLGLISAG
                     YDDGQNLNLAVESSLVKIFANNFIEIVN"
     gene            complement(16844..>18511)
                     /gene="asnB"
                     /locus_tag="L3M60_002001"
     CDS             complement(16844..>18511)
                     /gene="asnB"
                     /locus_tag="L3M60_002001"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HBN6226666.1"
                     /translation="VFNGNIYNNHEIKEELIKRGHSFTTGDDIEVLLASYIEFGKNCV
                     HKIKGMFNFIIYDRENESIFGARDLFGIKPLYYINKESAIIFSSEYKFILEYMKNLNI
                     NERSLQSYFSFQYVLPEDTMIQGIRLIPAGHYFRVENGILSLKRYNKLEFRSSTKFFY
                     TKNHLGNRDVDKEEVRNIVVDSIRNHMEEHKEIGTFLSGGIDSSIITTVASQINPNIK
                     SFSAGFSVKGYSELEVAKKTADKLGIENIQINITQDEYIKALPNVIYSLDDPIADPSE
                     VGLYFLIKEAGKHVKVALSGEGADELFGGYNIYKEYSTMKSVVNSPTYIKGILGRVSE
                     LMPNIKGRNYLYRATTPLEKRYIGNAKVFENSEVKRFFFKYKEKNIYEYLLSNLYRDA
                     QKNNYDYISKMQHIDVNTWLQGDILQKISKLSTAEQVELRVPFLYKDVFDVAKNLRME
                     QKINKNNTKVLLREAFREIVPEHVVQRKKLGFPTPIRVWLKDSLGGIVKETISNSNVD
                     EFIDKKYVIKLLDMHLKGHRDNSRKIWTIFTFCLWHQLFIEHKNVEY"
BASE COUNT         6082 a         3249 c         1942 g         7238 t
ORIGIN      
        1 actagaattg aaatttctag taacattaaa atttaattta ataaaataaa ttataaatac
       61 caatttaaat atgtatatat tattttataa atttaatatt ttataaattt aagtttttta
      121 ttatattatt ttagtatata tttaaagagt ctataaaaat taccactaga gattaaatct
      181 atatctcttt ttttataacc tctattttca agctctacta ctatgttatg tgctgtatgt
      241 gtgctttcaa gaccatctac acttggcata ctagagtttc cagtgaaaga acctatggtc
      301 tctttcccca agtaatcact aaaatcaaaa ccaaaagata catgttctat acctataaga
      361 tttaccatat aatctatatg gtcaaccaaa tgtattaaat cttgcttctt aggattacta
      421 ttagtaaatc ctctaaaact atttattcca actaatccac ctgtatttgc aatttctttt
      481 aattgattat ctgtaagatt tcttttaaca tcacataaac tcttagcatt agaatgagaa
      541 gctataactg gcttacttgc aatagacatt aaatcccaaa atgatttttc atttaaatgt
      601 gacacatcta ctatcatgcc caaattttcc attttatgga ctgctttttt acctatttca
      661 gttaaacccc tattttcatc tcctaaacaa cctgtagcaa gttcattctg ttcattccaa
      721 gttaatgatg catgtcttgc accaacttct ttataaaaat aatctattga atctatatta
      781 cttccaatat ggcttaatcc ttcaagacca attaaaatat ttattttatg ttcacttata
      841 cctttatcta tatctgaata tttttttact atatttacaa catctttcat atcttcaatt
      901 tcatctttta tagagcttat gatttgttta actctttttt caggctcttt atcatatggc
      961 gggtctatcc atattacaaa aatagctcct tctatatttg ctcttttaaa attatcaaga
     1021 tggattcttc taaatacatc tttttcccca ttaagccttc ttattgttac atctgcccaa
     1081 gtatctgaat gtccatcgaa aatcataaca acctccaatt aaataaaaat ctttctaaaa
     1141 aacaactttt tttcacgtaa aattatatca aaatgtttcc tactaaaaga ccaaaataat
     1201 taccaactat gtatccaaat gtaccaacta taagtattgg ccctactagc ttagaccatc
     1261 cttttgatat cgccatggct gcggctgttg taggtccacc aatatttgca ttagatgcaa
     1321 gaatagcttc ttctaaacta aacttaaata tttttgcaac tataaatgta actagcatgt
     1381 ttattagtac cattattcca cagtataaaa gtagtaatgg tgatttttga attattaatg
     1441 ttattgaagc aggaacgcct actacaacaa agaatatata tattaaaaat gtacccaact
     1501 cctgtgctcc ttttatgtca ccaaagaaat taggcataaa tgtagcaaac aacatagtca
     1561 aagtagttat tattagatat tgatttccaa ataaagtatt taacattgct aaaaaaggat
     1621 ttgataatgg tattatagct ttgaaccaag ttgatagttc tacagataca gcaacaatta
     1681 taaaggcact accaatggct attgctatat cttttagaga tatttctttt cttccccaat
     1741 aatttgctgc tactgtttct ccttcagtta cacctatact ttccatttca tctacatgtg
     1801 gatgtttaaa atgttttcta aaaaatccaa ttgatggtat agctattaag acaaagaaat
     1861 ataatgccat taaaagatta tctgctacaa ctgttgctga tactaattct cctggcgcat
     1921 caaatgcccc tgacatagcg gcaaagttta catttccacc tatataagaa cctgtcatca
     1981 tagctgcaac aatatttaaa tctggtacta cattttttaa tgctaaaaat ccaatcatag
     2041 ctccaagtac agttcccaca gaacctacaa gaaatataat gagcaatctt ccactttctt
     2101 tccatatctt tttaatgtta cattgatata acaataaagc tatggctaaa ggaacaacat
     2161 atccccatac ttggtcatat acaacagact ctactggtat aatatttaga tttgataata
     2221 acattgctcc tacaagagct attattgctc cagacacttt tgatgcccaa ttgtactttt
     2281 gttccaaata aatactaaat gctgcccatc cagttacaac tgcccataat acccaaatat
     2341 tatcaggact tacaaaactc ttcatcttaa ctacctccat ttcattaaat taaaatatcg
     2401 atatcaatat tcaatatatt gaatattaat actattataa tgcatctttt cagaaaattc
     2461 aatataacag aaaaaataca tgtattataa aacttttttt attttattaa tctttataaa
     2521 ttatatgtta taatcatgtc tgaggaggtt tgtatctaat gaatatagat gtcggaagca
     2581 aaattagatc tcttagaaaa tctaaaaata taagtatctc tactttagca aaaaattcag
     2641 atttaagtac aggcttaata agtcaaattg agcgtaatat ggttgttcca tctattgtag
     2701 caatgtggaa aatatcaaaa gccctcgata taaatatagg ttattttttt gaagagattg
     2761 gaagagagga ctctgatata gtagtaaaga aaaataatcg aaaaaaaata gtaactaatg
     2821 attctacaaa attttacgaa cttcttgttc ctaatttatc tgggaaaaaa atagaattta
     2881 tacttgttac tttagatggt catacacaaa aaaacagtga atttgtaaca catgaaggag
     2941 aagaatgtgg ctatataata aaaggtaaaa tgaagataac acttggaaat aaggaatatc
     3001 tattggaaga aggagatagt ttttatttta acagtactat cccacatgta tatgaaaatt
     3061 atgatgatga tgtatgtatc tcagtctggg caatgacacc accttcattt taacatattg
     3121 agttcttact taaatataaa ttgataaaaa tctgaaataa attagtatga taaactttat
     3181 tctaatttat ttcagatttt tctagcttat tatttatata tcagtcatta tatattaagt
     3241 agacagtttt catctaaaat gttggatagt atctcttcag ttccactcat tccattaaaa
     3301 gaacttgata aaaatgaatt ttctatttca tttttattta tatcaccatg cttcaataga
     3361 taagctttat ttgagatatt aagcatacca tagtccataa tagagtcgcc aattgcataa
     3421 aaatattcta ttcctaattc tttggtcaag tattttatag cattttcttt tgaaatttct
     3481 tttggtataa aatatatttt tctgccactc acataaaact tccaattttt tgattccaat
     3541 acatttgtat attctttaat tgattctaaa ttaaattttg agaaatcaac aactatataa
     3601 aaaaatgtat cttctgcaac tttaaaatta gttattccat ctacattcaa atagtcttta
     3661 aatttataaa tcatgctttc aatgggctca tagtcagatt ttagtttatc tattttttca
     3721 ctccatgatt tcaatatttc attatcttta agtataactc ctccatttga agttatactc
     3781 caaggaaaat aaatcccata tttattaaac tctatgcgct taaattgctc cactgttcta
     3841 gtagttgtag gtataaacat accataatat tgtatttgct ttattaaatt tatagtatct
     3901 aaagatatat aagatatttc tttttcccta taaatttcta tattagcata tttactatca
     3961 gcacttaaaa atttattgga gtaaatgata ctcctgtcta aatctgaaaa aacaatcatt
     4021 caatattctc ctgtctaata tatttcctaa acatctttca tattttttat tattccacaa
     4081 catttatagg cttttaaatc catttgttca acttcaacat ttttctcctt agctaatacg
     4141 aatatatggt ctaaagattt atcatttaag tctttaacaa gaatcttata cggaactctt
     4201 ctaagtaaaa cccttgtcgt ttctcctaca cctggtttga taaagttaat atctgttatg
     4261 ttatactttt cttttatatt taaaacatca aagtatccat ctcttgttat aatatcactt
     4321 tcccagtttt caacttcatc agatatgttc ttaaattgat ttttaaaaca ttcttctata
     4381 gtttcaatat agttcataga ctcatcttta tcttttaaat gtctataaaa cttagctcca
     4441 tggaagtcat ttttaccaat taaatcctct cttagtacag ttctgcttac tagaccagaa
     4501 acggttgaat ttaaacatgc acttggtatt aagaaatctt ctctaattcc ataaagtcct
     4561 gaatatccag cagggtcagc taatacagca agtgttgagt caaaatgttt tttaaattta
     4621 tcattaagct catcacatgc tttttcaagt tcttttgata tagttccttt accagtccat
     4681 ccatctaaaa attgaatctt tgagctcgga tgattcttta ctatatattc aattgcattc
     4741 atatctattc ccttatctct tattatagag attgtatagt gaggtaaatt tatattatac
     4801 ttgcttttta catatctttt tgccaaaata ccaataggtg ttcctgctct agcaagtgaa
     4861 acgagtacta catcatttcc atttttttta attatttttt cacacataac gcctatagaa
     4921 aatgcaagct tatctttatt ttctcttagt ttctcatgat ataaattcag ataatcttca
     4981 ctaacttcat actctattgg tatcatttct gaataatgtc taccattttg tatttttaat
     5041 tctctttctt tatttccttc ttcttcaatt aaatttgata tatcttttaa taaaaaaata
     5101 acatcatctt tagaatacgt actaaaatct ctcatatgac tcaactccta tcttaaaatc
     5161 gtgatatatt ttatcctgtt aaaacactct agctacaaca tactatagtt aattctttta
     5221 tacttgtctt tttaattata tcaataaact ctagataaga attttcactt ttatttagct
     5281 ctacaaataa ataacattta gaaaaattct gtttatctat attataaata taattttgaa
     5341 tatccatatt ataaaaactc tttagcataa atttacttct aataggataa ttatgcttat
     5401 caatattaat aattggactt cttgttgttg aatgatagta tacatcacat tgattttctt
     5461 tttcttttgc aaacagcata ggtatataca taaattcttc agtacctaaa aatagaatct
     5521 tttctttttc attttttaaa gattctttat tgtctatagt tattttttta ctttctctct
     5581 taacgatttc ttttaatcta aattgttctc ttctattaat tccaaatctt cctgtatatt
     5641 ttacatattt cttatttcca atgtaatctt ctaaatcaac ataaatatga tttactacta
     5701 aatttacttt atcacttaaa atagtatcta atttagtaat agtataatta tcgttataat
     5761 ttgaaataat atcttcatct atataaaatt taaagtttcc tttaaataaa tatacaaaat
     5821 ctatcttaca atctagctct ctttctaaac gagtaacttt acttaaattt tcatcactta
     5881 cccaattgag tattgaacat atagtgtatt tctttttagg atagatgctt tgaatcttcc
     5941 taattatgtt catgcatgta ttggctgtgg taatctcatc atcaaccaat attatctcat
     6001 ctccaaattt aaagttgtta agcataccaa agtataaatt ttggtctgtt gcatgagaat
     6061 gttcttctaa aaattccaga ttttctaact cgtcaaactg ttctctagta gtatgaataa
     6121 attcataatc tccacataaa taatcaaaaa aactatgccc aacagctgta ccagtttcag
     6181 caaaagctat aacagtacca tttgattcct ccttattttt ttcattataa attgtagcta
     6241 ttaattttcc caaattatcc atctcacttg gcttacatgc taaatgctta ccaagtttct
     6301 ttgagataaa caaaaaacgt cttttgctat tatttcttat tcctatgtct aaaaaatcat
     6361 ttagttctaa atttaatgga ttatcaataa tgtcaatctc acaattaaaa tttaagcaat
     6421 tcttcatagt ctactccttc atttaataca ccatatattt tagatagaat caatgttttt
     6481 ttagcccata aaaaatgtgg atttatttca ttcattctct ctcctaacac acccttactc
     6541 acaccatatt tactgtctgt actatccaat atatccaagg catcagtaaa atcttcatat
     6601 gatactgtag ataacgcttg tacaatttga atttgcattg gatgaattac agtctttcca
     6661 attaatccat gatatttatc taataatatt tcttctatat acttatttct tatgtcctta
     6721 tttttcatat caaaaaattc tgacactcca ccacttatag gtatatctag ctctggtctt
     6781 tgaagatatg tgattatatc tagtataaat gaagaacaaa tcaaattatt atatatagaa
     6841 aagtttttat ctctccttat accatacatc cctaatatat cagttatacc tactctaatg
     6901 cttaatattc ttgacttatg tcttaaaata gcattgtaca aattaataaa actaagttct
     6961 tttttttcat tatatataaa ttctcttgtc tctattattg gaataacata catatcttga
     7021 agattcatag aatctaaagc ttctatacaa ttttcaatta attccgagtt tgctttagga
     7081 attaatattc cggttataga atgactattt ttaattataa tctctttaat tctcaacagt
     7141 tgctccacat cttttattct tacaaaaatt agtggtattc catctttatt agttatactt
     7201 tcattgctaa tattttttaa aattaatcta aggttttcta tagcctctaa ctccccattt
     7261 actccaacag catcttctaa acaaatagct aatggtctaa ctcccttaac atcccctata
     7321 atactcttat aaatcatatt atattgagtt gctggaacgt ataaaaaagc tcccagtgca
     7381 tatttcagta catctttttc agtcttagca ctaaagtctt ctggctcttt caaaaaaatt
     7441 ctctccaaat catctttaca gatataatcg aaatatttca atgtctagcc tcctcatata
     7501 tagaaagggc caactttata taaattagcc cttaatataa tactacttat tataaattat
     7561 agttgtattt aattcttcca atctctttgt attatcttta attaaagctc tattgttttc
     7621 ttctattctc atagtttcat ctataccaga cttgatagta ttgtacatct tttccaatgt
     7681 ttctatttga acagaacttg ttccagccat tctagctatt tgtgcacttt gagaacttac
     7741 attttgtgca tttctaagca ataattcatt agttttgtca tcaagtgctt ttagagattt
     7801 tgcttgtaat tcttgctttt taagtaatat agcttgagat aaacattgtt taaatactgg
     7861 taaagttaca acaaatgcac tatttatttt tctaattaaa ccataattat tatgttgcat
     7921 acctctaagc ataggtattg tttgaatagc tatattttct gctattctta aatcatagac
     7981 tctttgattt aacatatcat aaacttttaa tagttcttga taattaacaa catccatttg
     8041 atctccacta gtctctgatt tttgtttaaa gaaaggtagt atctcattat ccatttcttc
     8101 tattgccatt tctccagcaa caatatattt ttgaagttca ttataaaact caaagttttc
     8161 atttaacata gcacctattt gcttattaga aagcgctata tctctttcgt attgctcaag
     8221 ctcaatttga attttttcaa cttctcctcc taatgtttca tacttttgaa acatcatttc
     8281 aatggcgtta ttagcttttt tgaaaagttt ttgaacgaaa tttggttcct tagtctcttg
     8341 aaaatcatct aaatcaaact tgtccattat tttagttaaa tgaaccaaca ttttagaatt
     8401 ttcttcattt ctatttaatt ttattgtatt taaaaggcta tcagaaactc ttgctactcc
     8461 ttcagatgct tttctaccaa actgtaaaat cgtgtctgga ttttctactt ctataagaga
     8521 agtcaatgcc tcaacttcct tagactttct caattcattt tttttattat ttgtatattc
     8581 cattatatcg aaattttctt cttgaactgc tgtgggcaaa ttctcagtaa catctacaga
     8641 gttcttatct tttgaaaaac tatttatatt tatccccatc taaaatcgtc tcctctttcc
     8701 aaaaattttt ttaagtaaag gtatatcatt ttcaagcgaa tttaatttaa ggtcatatac
     8761 cattttatcc aatctgtgta catcaatcaa ttggttattt ataacattct ctatattatt
     8821 ttctcctcct ggacacagga taataatatc aaatccaatc ttactaagta aaagcataag
     8881 aaaaactatt tctttatcaa atataatact cttatacata tatataatca attttggatt
     8941 tataagtgag taatcaaaat tgttcatcat gtgaacaaac ttcttatcca tgtgaagtac
     9001 tgtaaataga ccttttatct tgtcttctcc acttaaacca ttcaaaaaca tacttccttc
     9061 taataactct tccacttttt ctaaaatcat aatttgttga tttaatgcta gtgttgatat
     9121 ggaataatct ttattattta aaacagagtt tttatctatt acaccttttg aatccaataa
     9181 aaaggataaa ctaaaagctt ctcttgtaaa agcttttgaa aatctatcta catctccatt
     9241 aaattcaata aacgttgaac tttccgaatc tcttaataaa tctaaaaact taatatattc
     9301 attcttatca ttatatattc cattaatctt agaaagaaaa tttggagctt ctacaatagc
     9361 atcattaaaa ttaaatcctg gtcttaattt aagtggctga ttccagtata ttgatatttc
     9421 atcaacggtt gaacttagaa gtagattttt tatttttcta tcttgaagtt gccaaggttt
     9481 tataaatcct gcatcattta acaattcctc acttatacgt tttgatgctt ctgccccaac
     9541 agtaaaagct ttttttacag aacttttatc tattttttca cctaaaataa ccctttcttc
     9601 aaaagaagtt ttttcttcta ttatgttggc ttcttcaaat tctacattgt atctttctga
     9661 ttttaaaata tctatattac tctttgaatt tggattaaga tataaaacat caaaacctgc
     9721 caagtacaat attaagagta aataaacttc atgttttttt atatctccat aaaatattac
     9781 ttttggagct tcatttttat ttatatctaa agcaccgata taaattttta tccatgccat
     9841 tattttcact ataaaatttt gcttaactct atcattagta aattcttttt cttcctcttt
     9901 taacacttta tccattgctt gctttatttt ataatcaatc tcttcaaatc ctaaaatatt
     9961 tatatctaca tcttccatac tagttctata tttttgaagt ccatttttaa aataatttat
    10021 aatatcaaaa tcactaggat ttgctataac atcagtaaat aaagcaatat cttttttgct
    10081 atcaatattt tgttttgtct taaatatctc gagattgtat aaaacttcat cttctaaacc
    10141 aatatatgaa ataaaataac ttagatatcc ctttgaagga gtataatttc ctctatctga
    10201 ttggctggag aatatttttt caaatacctt tgagtcaccc ataactatcc tcccaattta
    10261 aatgtttatt cagaactgac ccaaaattca tattttgagt cagtctgttc tcttctaaaa
    10321 aatttgtttt aagcagtctt ttttggtttt accagtctta ttatacattt tacaacttca
    10381 ttaaccacaa ttacacttgc tgctacacat agaactttta cccatgtcat aaaatccatt
    10441 gctacagagt taaagaaatc ttggaataac tgagtaaata ttacttgtgc aattcctgtc
    10501 aatactatta tttgcagtgc taatttgtta tgtgtaaaat tagggaaaat actatctgca
    10561 ttaaattctc tacagttaaa cgcattaaac agtgcactaa aggcaaataa tgcaaacatt
    10621 actgttccct tatgagcttc gtcaactcct aagaaattaa atgtagattg tagcattagt
    10681 attgctgtta tatagaatgc atttaatact attgtataca ccattgactt agaaattata
    10741 ttggcttttc tatcaacagg ttttctattt aatacatggt ctctaactgg ttctagacct
    10801 aatgctagtg ctggtggtcc atccatgatt atgtttaccc ataataattg tattgtagta
    10861 aatggcatct cttgtcccat aacttgagat attatagcca ctaaaaatgc aattacattt
    10921 acagtcaatt ggaattgaac gaatctttgg aagttatcat aaattcctct accccattta
    10981 atacctttta caatggtact aaaactatca tcagttaaaa ttatgtccgc tgcgttttta
    11041 gatacctctg taccagatat acccattgct ataccgacat ctgcttttga aagtgctgga
    11101 gcatcattta taccatcacc cgttactgca acaacatctc cgctcttttg tagagctgaa
    11161 actattctca ttttactatc tggtttactt cttgccacga ttgaaatact ttgtatctct
    11221 tcttttaact cttcatcatc taacgtatct atatatgaag cttcaaccac cctcatatta
    11281 ttttttaata gacctaactc ttcacctata gctcttgctg tatttatatt atcccctgta
    11341 agcattttta cagaaacacc agctttattt gctgtattta cagactctat aacatctttt
    11401 ctaagtgggt ctcttatgcc aacaaaacca ctaaatacta aattattttc cattgcactt
    11461 acattcatag catcactttc ggcagttact gctgcttctt cttcagcttg tgagatttct
    11521 ttatatgcaa accctaaagt tctcattgat tttatttgca atttctttat ttcttctaaa
    11581 atctcatgtt ttatattttc agttaatgga actatgtctt ttcctctttg aatatgtgaa
    11641 cataactcaa gtaagacttc tggtgctcct tttgataata atacatgatg attatcttga
    11701 cctataacag aagtcattct ctttgaatct gaagtaaaag gattttgagc tactatatta
    11761 gaattttttc tagcttgtct ataatctacg tttttgtaat ataaaagcaa tgcacattca
    11821 gtagcacttc ctaaatactt aatatcacca tcattgtgct ctacatcagc tgtagagttt
    11881 atcatacagt tttcttcaaa ataactattt aattcctctt ctgaactttc aatatatctt
    11941 ccatcaacat atgcaacttc tacagtcatt ctattttcag ttaatgtccc agttttatct
    12001 gaacatatca cagaaacaga acctatcgtt tcacaagctt cttttttagt aaccaatgca
    12061 tttattttag ccattttttg cattgttatt gcaagtgtca tatttatcat agttggaaga
    12121 ccctctggta ctgctgcaac tatcaacgca acacatactg taaacgctgt tttaactggg
    12181 tctattgatt ctaaaaatgg taatattcca gacatatcta catttaatac attcttactt
    12241 accatttgga aaaccataaa tataaacaat aatcctgcta ctgcactaga aaccttggct
    12301 atcaagccac ctaaatttcc aagttttatc tgaagtggag tttgctcatc tgtttctgat
    12361 aggttttgag caattttacc catttcacta ttatctccaa ttgaagttac aactagagct
    12421 cctctaccat aagctacaag tgtaccacca aacaccatat ttatctgttt tgcaggtatt
    12481 ggctcttgta ctactactgc tcctttagat tctatctctt ccatagaaac tactacatca
    12541 cactttttag atacatcatc agattctcct gttaacatat cttctctaat tttaaggttt
    12601 atactttcta taagtctacc atcagcaggt accatgtcac cagtttcaat atatactata
    12661 tcacctggta ccaattcact cttagaaatt tgatgtactt ccccatctct caatactttt
    12721 acttctatgt tttcagtcaa cttagaaagc gcttctgctg ctttcttaga acgaccttct
    12781 gtaatcatac ctattgctat acctattgct atagcaccta caatacctat tgcatcatga
    12841 gtttccccaa ttactgcact tactactgct gcaataatca agataactat cattggttca
    12901 ctcaaacttt cctttaagtc atcccaaaaa ctaccctctt cttttggagt aaattcattc
    12961 aaaccatact tttctcttct agtcttaact tctgattctg ataaaccttt ttcaggatta
    13021 acagataagt cttttgtaac ttcttcttta tctacattat aatatttgat atcattatta
    13081 aattccatat gcacactcaa tcctgtctaa attttgcatt acttctttaa gacccgcatc
    13141 ttttgttcct tcaccaattg ctttaaattg ccaactgcca tcttttctat atatagctcc
    13201 tactataaga gtagtttttt cagaataatt atcagataga ttatattttg ctatctcttt
    13261 cttattacct tggtcttcaa ctcttatata ggcattttct atcattccaa agtgttgttt
    13321 tctatccaca caattgtata tatttactac aaataataat ttatgaatat ttgatggaac
    13381 tttatccaaa ttaactagaa tttgttcatc atcacctgca ccctcaccag ttagattgtc
    13441 tcctgtatgt aaaactgact tacaggcact ttttagattt ccaaaataaa ttaaatcttt
    13501 tattccactt aatttacctt cttgattaag cataaataca gatgcatcac aatctatatc
    13561 agcttgtcct ccaccaaaca acgatttaaa aaagcctcca ccagattgtt gcacagggtc
    13621 ccatcctaga cctacaagta tgttactcag tcccggatta gattttttta aatctatttt
    13681 atcacctttt gataaattaa tcgacatact aaacctccac aaaattttat ataatataac
    13741 ctatacatta tttgtatagg ttatttatat ttaatatatt atattactta aattaaacat
    13801 ttaagccata gtttctacaa agagcttcta agccaccttc aaatcctgct ccaactgcac
    13861 taaacttcca ttctccatta tacttatata tctcacatac cgtaattgca gtttctatac
    13921 taaattcttc acctaagtca tatcttaata tttcttcatc attttcagaa tttaatactc
    13981 ttatatatga gttgcttacc tgtccaaaat tttgtcttct ttcagcagcc tcatatattg
    14041 taactgttac agctatcttt tgtatatgct ttggcattgc ctctaaattc accaatattt
    14101 cctcatcatc tccatcacct tcaccagttc tattatcacc taagtgttca actgctccag
    14161 atggatgttt taagttattg tagaatataa aatcttcatc tttttcaact ctttgagatt
    14221 ctccaaccat gaaaacactt acatctaaat cataatcata gccactatca aatgaatttg
    14281 tatcccatcc tagtccaagt tttatatttt taagccctgg atttccctta gtcaaatcga
    14341 ttttttgtcc tttttttaat actatagcca tggaaaatat ccccctttag tttattatta
    14401 tagttgaaat tataagttta ttccaaagtt tccacataat gcagcaagac caccttcaaa
    14461 accagaacct agtgcattaa acttccattc tccattgtgc ttatatattt cagctactac
    14521 tatagctgtt tctatactaa aatcttctcc taattcatac tttattaact cttcatttgt
    14581 gtctttatct actattctta tataagaatt acttacttga ccaaaatttt gtcttctttc
    14641 tgtagcatca tgaattgtaa cagtaaaaga aatcttactt atttctccag gaatcttaga
    14701 taaatcaaca actatttgtt catcatctcc atcaccttcg ccagttctgt tatctcctaa
    14761 atgctcaact gctccagatg tatgttttaa attgttgtaa aatacaaaat ctccatcatt
    14821 agtaacactt ccacttgctc ctgttaaaaa tgctgctgta tctaaatcaa agtcaaatcc
    14881 accatcatac ttgttagtat cccatcctaa acctactaaa atatttttaa gacctggatt
    14941 tcctttagtt aaatcaacct tttggccttt tgataatgtt atacccataa tttatcgctc
    15001 ccttgttaat tttagctaaa caaatcttgc taattattta tctttttctt taatcatatt
    15061 ataacatttt ttattcattt gtgaagaaaa cttgcttgat atttttaaaa tataattaaa
    15121 aaaatgttta tatttcttac tttttacata gattttacat actttattaa atgtataaaa
    15181 gctatgttta atatgtaatt atatatcatt tatataaaca agtacatatt ttattttgaa
    15241 actacaaaat tattgtacta tattgtttta agttgtttta taatagatta tatcatctta
    15301 ttataggaga aaaaatatga tttgtactat taaaccacta aagatttcaa taaaaaaatc
    15361 aaatgaacct ctaattgaat ttatgtctaa tactacctat caaaataaga gttcttcata
    15421 tattaaagaa gtctacttta caagatatat aggtgttgat gattcaattg ataaatacat
    15481 agaaaaaatt aaaaatatag atacttattt ttttaataat cctataattc cctatattag
    15541 attaagcagt ctaaacataa attttgacaa agaacaaaca gataagatgc ttaaaatttt
    15601 taatatttat gatagtgcta aatttaacag ttgggattta tataatatca acttcccatg
    15661 taaaatagaa aatgatactt taaattggac taaaaagatt gcatttaaaa atactttaga
    15721 tttattttcc atgagtatgc caaattgtac accctctatc atcaaaaact ttgcagttaa
    15781 acttttgtgt tggatagaat attttctacc taaattattt tataacaagg tgaatactaa
    15841 tatttcacca aagatattgt attttggaaa tattaaaaga caagaacttt ttttcttata
    15901 ttttttatca caacttgggt gtgatatttt atatataaat ccaaataaag atattcttga
    15961 tttatatcct gaatgttcaa agttttctaa tatatttgaa ctttctagga aaactccaaa
    16021 tatattagaa ataccttttg aaaggcatat tgcaaatata aattacaatg aaaataccaa
    16081 taagacttcc caccctatga ataaacctaa ctctgaaaat acgtttaata acacaaattt
    16141 aatatctaaa aaaatcgtac aaggtgagga agtagaactt tcctatgaag aacttgcaaa
    16201 gttatcagct tctgttgtta tgattgttgt gtgcaataaa gaaaataaac cgtttaaaag
    16261 cggttctggg gtagtaataa ataatgaagg ttatatactt acgaatcttc atgtagtcaa
    16321 tgatggttat tcatttttag ttagatttga aaatgatgat aaagtttaca catcttacca
    16381 aatcataaaa taccattcag gttatgattt agctgtgata aaagttgata gaaaatgtaa
    16441 gccaatacct gttaaaatcg gtaaaaaacc tgtaagaggt caaaaaatag ttgcaattgg
    16501 aagcccttta ggtcttttta atacagtttc agatggaatt atctcagctt ttcgtgattt
    16561 tgaaacggtt caaatgatac aatttacagc tccaatctct agtggtagtt caggaggagc
    16621 acttttagat atgtttggaa acttactagg attgatttct gctggatatg atgatggaca
    16681 aaacttaaat ttagctgtag aaagtagtct tgtcaaaata tttgccaata attttattga
    16741 aatagtaaac taatcaatca aagttctatt tgtactattc cataataatc aagagggaaa
    16801 aaacagtaca tgtttttgcc ctctgattaa tatgaaaaca taattaatac tcgacatttt
    16861 tatgctcaat aaacaattga tgccatagac aaaatgtaaa tatagtccat atttttctcg
    16921 aattatctct atgcccttta agatgcatat ctaaaagttt tattacatat tttttatcta
    16981 taaattcgtc tacatttgaa ttagagatag tttcctttac aatccccccc agagaatctt
    17041 ttaaccaaac tcttattgga gttggaaatc ctaatttttt tctttgaaca acatgttctg
    17101 ggactatttc tctaaatgcc tctctaagta aaacttttgt attattcttg tttatttttt
    17161 gctccattct taaatttttt gctacatcaa atacatcttt atataaaaaa ggaactctaa
    17221 gttcaacctg ctcagctgta gaaagtttgc taattttttg aagtatatca ccttgaagcc
    17281 atgtattcac atctatatgt tgcattttac ttatataatc gtaattattt ttttgtgcat
    17341 ctctatacaa attagacaac aggtattcat atatattctt ttccttatac ttaaaaaaga
    17401 atctttttac ttcgctattt tcaaaaactt ttgcatttcc tatatatctt ttttctaatg
    17461 gtgttgtagc tctataaaga tagtttcttc cttttatgtt tggcataagt tctgaaactc
    17521 ttcctaatat acctttaata taagtcggcg agttgacaac tgatttcata gtactatact
    17581 ctttatatat attatatcct ccaaataatt catctgctcc ttctccagac aatgctactt
    17641 taacatgttt acctgcctct ttaattaaga aataaagacc tacttctgaa gggtctgcta
    17701 tagggtcatc caaagaataa attacattcg gtaatgcttt tatatattca tcttgcgtta
    17761 tattaatctg gatattttct atacctaatt tatcagctgt tttctttgct acttccaatt
    17821 cactatatcc tttaacacta aagcctgcag aaaaagattt tatatttgga ttaatttgtg
    17881 aagccacagt agttataata gatgaatcta ttccaccaga cagaaatgtt ccaatttctt
    17941 tatgctcttc catatgattt cttattgaat ctactacaat atttcttact tcttccttat
    18001 ccacatctct attacctaag tgatttttag tataaaaaaa ttttgtacta cttctaaatt
    18061 caagtttatt atatctcttt aaagataata tgccattttc taccctaaaa taatgacctg
    18121 ctggtataag ccttattcct tgtatcatag tatcttcagg aagtacatac tgaaatgaaa
    18181 aataactttg taagcttctt tcatttatat ttaaattttt catatattca agtataaact
    18241 tatattctga ggaaaaaatt attgcacttt ccttatttat ataataaagt ggttttatcc
    18301 caaataaatc tcttgctcca aatatacttt cgttttctct atcatagatt ataaagttga
    18361 acattccctt aatcttatgt acacaattct taccaaattc tatataagat gccaataaga
    18421 cttcaatatc atcacctgta gtaaaactgt gccctctttt aattaattct tcttttattt
    18481 catggttgtt atatatattt ccattaaaaa c
//
LOCUS       DAFNNB010000068        18489 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0069, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000068 DAFNNB010000000
VERSION     DAFNNB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18489
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0069"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            188..1552
                     /locus_tag="L3M60_002002"
     CDS             188..1552
                     /locus_tag="L3M60_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBN6226667.1"
                     /translation="MDIKELVKIQRKYCNTGKTRDISFRIEQLKKLKSVVSQNEEKIL
                     LALKRDLDKSDFEGFMTEVGMFYSELNFAIKNIRKWSKIKRVKSSMVNFPSISKVVPQ
                     PYGVTLIMSPWNYPFQLALIPLVWSLAAGNCVILKPSEYSVSTSSIVKDIIENAFSKE
                     YVAVVQGSQEESEKLLLERFDYIFFTGSTNVGKIVMKSASEHLTPITLELGGKSPCII
                     LKDANIDLTAKRLTWGKLINAGQTCVAPDYVLVHEDKKNELIEKIKYYTNKYFGDNPS
                     ANEQFPKIINQRHFSRILSLIDKDKVIYGGNYNKETLKIEPTIVDNVNWDDNIMKEEI
                     FGPIFPILTYKDLDEVIQKIIQMPNPLALYIFTKNKYLENKILEMIPAGGCCINDTVT
                     HIATNYLPFGGIGESGMGSYHGKAGFDTFTHYKSVLKKLNLDVPIRYAPYDNKLIKIL
                     KKIM"
     gene            1691..2857
                     /locus_tag="L3M60_002003"
     CDS             1691..2857
                     /locus_tag="L3M60_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBN6226668.1"
                     /translation="MKINWNTKYTTIAIYTFIIAASSIIFYLVSSQIDVFSNNLDAMF
                     TTLQPFIIGFAIAYLLNFILKFYEDRIFIKSEKLKKLKQSSKRGLGILLTYATAMLIL
                     YLFMYFVLPQVIESIVGLANDIPMYVNNATKLIDKLMTDLNLDEQYFNLAVDKWNEFV
                     TYIIKFVTDLIPILGSMLKNVASSIWNIVLGLIVSVYLLIDKEKFYGLSKKITYAVFT
                     EKQAARILELTHRSNYTFGRFLGGKILDSFIVGILTFVILTLVKMPYTLLISVIIGIT
                     NIIPFFGPLFGAIPSTLIVLFVSPIKAFWLLLIILIIQQIDGNIIGPKILGDSIGISA
                     FWILFSLLVAGKLLGFIGMVIGVPMFAVIYSIIKDIVEGKLDKKGLPTDTSDYM"
     gene            2974..3756
                     /locus_tag="L3M60_002004"
     CDS             2974..3756
                     /locus_tag="L3M60_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBN6226669.1"
                     /translation="MAFIECKFKSKYTGGETDIIVILPVANGKELFSGKEIYDFEGKF
                     KTLYLFHGLEDDQTSWIRYTNVEHYAKNKNIALVIPRVETSFYTDMVYGHQYFTFVSE
                     ELPKFVRAIFPLSDKREDNFVAGMSMGGYGALKLALSKPKEFSAVASFSGSPDMIHEL
                     ENQQIETNADILFHDFGRPEDARNSENDLLYILKNLKERNEDIPKIYQFCGDRDFLYK
                     NNQMFKKYAESLHVDVEYEECVGGHEWRLWNLWIKKFIDIIG"
     gene            complement(3875..4528)
                     /locus_tag="L3M60_002005"
     CDS             complement(3875..4528)
                     /locus_tag="L3M60_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6226670.1"
                     /translation="MSKSEKGKETKASIISAARELFFEQGYHKTTTRQIAERANINLG
                     LISYYFSSKSEIGAIIYEDIRNEMQSLIYNYHEEGTMMMFFLAMNADLCFLLKNEAYR
                     NFYLDIAYEEAILSYQRSVTEMVMKRYVNDKESIEHDELILSCIGILAMKPEIIRVYT
                     SHKYDISSQAVIKHFTKQFLINLGHSTDLCEEILEELSKYYIDIVDNFTPIITRVRT"
     gene            complement(4552..5916)
                     /locus_tag="L3M60_002006"
     CDS             complement(4552..5916)
                     /locus_tag="L3M60_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HBN6226671.1"
                     /translation="MLLLIVTCAIYIWIGFYFSKKVKTASDFLIAGRNLPLPILAATI
                     AATSFGGGCLVGGVQWGAERGLWVGMYSTIGAALACFINAFFAGKLRSLSCDITPADY
                     IETRYGHSIFLRAYQSLVTPISILAIMGSQLISFGSVSTAFGIPYDIAVIIGVIVIII
                     YTYTSGMWGVAVSAFIQLAICIVFLPIVAYISLKLVGDNPLLMLQSMVKEPFFPGNKS
                     ISDFMYTVLPLVLGSIFSYESFLRYQCADNAKNAVKSSVIAGFILLFLAFPIGIIGAI
                     GGSLFPNISSVQVLPQMISTTLPPLISVLFLSAILAAIMSTADSLMTSMSAIISRDIY
                     NKLLHPNIEFNDLKNTLKYSQIASALTAIIGAIIALKFDSLLELLFWPAPLCTGVIFA
                     PFVIGLCWKDATKKGAIYAVIVSVILALLNMIGILTVFDRILVPIIGGSITIFIVSKF
                     SKKD"
     gene            6565..7995
                     /locus_tag="L3M60_002007"
     CDS             6565..7995
                     /locus_tag="L3M60_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6226672.1"
                     /translation="MSSQESKPKGNFISNFRDDMRDPKKRAIYTYSSSINLAYNFIGY
                     FVSTVTNIFLTDVAKLGVIMAGGMQIIQSFIKIAIAPITGTVFDKQPFKKGKYYPWLR
                     YAPAALALTYGIFFIVPLMKISPALLLPLMTALLLIASCAQQVVFTIIYAIYPVVAKT
                     PRDRVIGSTTTNIFKEVGKFLVGFSYPLLLVLFADVLGGEGMGYLGTYLFFAAGCLLV
                     FWFSSTEIIKSGAEEEVLQKEEKPKIKASEMAKALFTNVPLMMAFCLEFLICIRSIGL
                     GPLAPYYFKYVIGDERGLAVFLSIMPLVSVAFMFFAPVFIKICREQKLASIISFSICA
                     LCHLLVAWTPWGKTTLGVTMLLAIGGGFSNVVSIINLNFFAGSCDYGHWKSGKDLPGL
                     SMSLYPVAIQVGVLLATTIRTVLMNSMGYQADMVVTEAVKSGFINMISYSMAIPLIIA
                     VVIAILYPVSDKKLNQIREELNQRNA"
     gene            8098..9201
                     /locus_tag="L3M60_002008"
     CDS             8098..9201
                     /locus_tag="L3M60_002008"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBN6226673.1"
                     /translation="MIEKLLRNTVKELHQYVPGEPIEKVKEKYGVKEIIKLASNENPL
                     GPSPKAMEAMIEMLKQGQLYPEPEANELRRKLAEKLDLKPENFIVANGADNVITLIGE
                     AFINRGDEVIYCNPTFPSYRTATIKNEGIPVEVPLTEDYKYDLQGILDKITDKTKLIC
                     VCNPNNPTGTIVDDKELEEFLKKVPENIITILDEAYIEFLTVPNYVDGLKYVRENYNV
                     IVTRTFSKIYGLAGLRVGYGIAKDEIIRTLFTVKEPFSANRVAISGATAALDDEEFIK
                     ETYELNRVGMAYFKEEFTKMGFDVVDSQSNFLYVDMKTDIPKLFEDLKKRGFVIRPSA
                     THARVSIGTMEENKKFVAVLKEILNIKEEPSIG"
     gene            9216..9452
                     /locus_tag="L3M60_002009"
     CDS             9216..9452
                     /locus_tag="L3M60_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6226674.1"
                     /translation="MKRGVDMNLKINAERCKSCEYCVISCKKGALKISSKINKEGYAH
                     VEVDESKCILCGICYQVCPDNVFEIIKEEACQEA"
     gene            9469..10518
                     /gene="vorB"
                     /locus_tag="L3M60_002010"
     CDS             9469..10518
                     /gene="vorB"
                     /locus_tag="L3M60_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBN6226675.1"
                     /translation="MKKMWKGNHAIAEAALRGGCEFYAGYPITPQTEVMEFLSHRMSE
                     LGRTFIQSENEMAAIYMVYGAYASGMRSMTSSSGPGISLKQEGISYLCANHYPCVIVN
                     VQRWGPGLGSLDSAQTDYLRDTRGGGNGDYHLIVYAPNSIQETVDLMYNSFDVAEKYR
                     VPVEILTEAALGQMMEPVEFPEFKKREEDLGWTYDGSNRDHAKVADNQKPTFCMEKRM
                     RISENEQQWEDYQIEDAEYVFVAFGIPSRTTMNAVRRLREQGEKVGIIRPITVWPFPY
                     KAFEKVSKDVKGFISVESTDTGQLVEDVALASKKVCKENVPVYGLFSGNHIPKTLQVM
                     DTYSKIKSGEIKEVF"
     gene            10520..11260
                     /locus_tag="L3M60_002011"
     CDS             10520..11260
                     /locus_tag="L3M60_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBN6226676.1"
                     /translation="MATEKLTPSVVSIPNKFCPGCGHGIVNRIIAEVIEEHGYEKNHV
                     LTLGVGCVCNMNFSWNGDKMQTAHGRASSTAIGVKVALPDTLVMTYQGDGDAYVIGLS
                     ETLNTAYRNHNVTVFVINNNNFAMTGGQMSWTTMPGQVTTTSANGRDISTTGSPLKVP
                     EMVANFFDVAYVARGSVHSPKEIINLKKYIKNAIEAQLNGEGYSLVEILAPCPTNWSV
                     SLEKSIKWMEEEIVPYYTLGEFKQRDGE"
     gene            11263..11802
                     /locus_tag="L3M60_002012"
     CDS             11263..11802
                     /locus_tag="L3M60_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBN6226677.1"
                     /translation="MEKELVFAGFGGQGVLTLGLIVAESALELGKQVTWMPAYGPTMR
                     GGKAYSVVKFSDESIGGPDMEEIDVLVAMNKPSLDYINLVKEGGTVVINTSAIDENIA
                     LREDISVVKINCQELAQKVNNPKAANIVVLGALIAKTGLFEKELALKTMCDFFEEKGK
                     GKFNIQNEAAFMEGYNNAI"
     gene            11926..14025
                     /locus_tag="L3M60_002013"
     CDS             11926..14025
                     /locus_tag="L3M60_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HBN6226678.1"
                     /translation="MSLEKLIRPKSIAIVGVTDKLGFGRSAALSIVKSKDTDRVYYVN
                     PKREELFGRKCYKTIQEVPEIVDCVVVCTPRNVVPSVLKDSAELGTKAAVVYASGFAE
                     EGTEEGTDLENQLIEISNTYDMKILGPNCMGLLNCIDKVNLWAGGSKWDLDTKKPGIG
                     IVGQSGFITAEIVSSDYFNISYGFSTGNGNIVTLEDAVDFLVDDNYASVIAIYLEGLK
                     NPQKFIDALKRAAQKRKPVIILKSGRSEKGAISAASHTGNLAGSSKAFESIFEKYGVI
                     SVENLEQFMCLAQAFSVLDGNLPTNSNFAAINFSGGENTICADLAEENGVELAEISSE
                     TKEEMKKYLPGFATPKNPLDATTALFHEKDMIVGLLHTFEKDSSVGFTMVGANIRDEE
                     NEMHETLCQAVSEAREQGLKKPVFAVPTLEGNRYLDYRNRLEYNQVPIMSSVGTTFTC
                     FNKMAKFIDYDYSKRTLEFKAVKKRESNNVVALSELDSKIEMKKYGVPVPGQGNAKSI
                     DELDEILKYIKYPVVLKINSSEILHKSDVGGVKIGIKNRDEAVDAYNEILTNVKKAKP
                     DANIDGILVQEMVESGIEIIIGITNDDQFGPMLLVGLGGVFVEVFKDTTLYPLPINHD
                     EAIMMLKKLKSFKLLNGYRGSEPCDIDALADMMVKLGKYAYENKDEVKEIDLNPVFVY
                     PKGKGVCAVDALIVKYK"
     gene            complement(14292..14807)
                     /locus_tag="L3M60_002014"
     CDS             complement(14292..14807)
                     /locus_tag="L3M60_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HBN6226679.1"
                     /translation="MISAKMEKLLNEQINHELYSAYLYLSMSSYLEAEGLKGFSNWFY
                     VQYKEETDHAMFFYKYLHNVGGKVQLDAIPMPDSDFTSAMDILERTLAHEKKVTALIN
                     NLAAVANSESDFRTSQFLLWFISEQAEEETNCEDNIKRVKLAGEGGLFFVDQEFANRT
                     YSAPTNPPVTI"
     gene            complement(14899..16110)
                     /locus_tag="L3M60_002015"
     CDS             complement(14899..16110)
                     /locus_tag="L3M60_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HBN6226680.1"
                     /translation="MSNKQGSNIKDVKIKNKLIILKLLSTNTPMSRVDLAKSTGLTKM
                     TLSNLVTELINENMIKEIESSTLNAHSSGRKPILLDISNISPCICGILIRRNICKVII
                     SDLKGNIVKQLSETYPKCLSNDVLKDIIKRMFTKIMKINKRDIIGIGISSIGPVDDIN
                     GTILNPPDFGNVSNLNIVAFIKELSDLPIFLINDANSGALAEKMYGLGKNISNYIYLH
                     IMNGIGAGLVLENKLHTGNLGQSGEIGHTSINFSGPLCSCGNNGCLDYYTSVSNLIKR
                     IESLSHIYPNSPLINCKDFSLVKLIDEANNKDSLAMFLLDEFCTYVSYALVNTLTLID
                     CSSIIIGYDFNIPGTFIENTLLKKLTVSASFSKYKKISVRHSNFGANAPLIGSIAIVS
                     YNFFNGSINFY"
     gene            16768..18210
                     /locus_tag="L3M60_002016"
     CDS             16768..18210
                     /locus_tag="L3M60_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6226681.1"
                     /translation="MRTIRRVLALGLTLAIFLINVPNVDALTSDTIKGNNIYETAGSI
                     ADKKSYDTAIMVNMDNSIADGLSASGLAGAVDAPILLAQKNKIPSETKQRLKNVKKIY
                     IIGKELSISKSVENELKNTGAQVTRLGGDDRIKTSYSVAKEVNGIKKVDEVILTNAYK
                     GEADTISAAPVSVRDIAPIVLTDGKSVPFSTSNVKTYAVGGSISMSTSLVNKTNAKRL
                     GGSDRYDTNKKVIKEFYPDASEFYLSDGYDLVNALTGSTIAKENPIVLVSESSDKSIL
                     AGADKITRLGSISDSVYNKCVSVAQNNGDSSTKGDSPMKNETSILGQPTASLEACLKW
                     AKSKKANDLFIELIPILYDTAVQEGVNPVLAVAQSAKETGFCNFGGVLDASFKNPCGL
                     KTSVGGSDTDKNAHSRFDTWEEGILAQIQHLCLYAGQDGYPLSNPVDPRHEKSLFGKA
                     KTVESLSNNWAGGQYGQDLVRMMGEIEATK"
     gene            18252..>18489
                     /locus_tag="L3M60_002017"
     CDS             18252..>18489
                     /locus_tag="L3M60_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226682.1"
                     /translation="MKKSNIFSIIFPTVIMVLMFLTFVTDIFNLADVQSKSIFVVGMV
                     LIFPISFLIQGIICVLNKTSWILSLIVSLLTYIVL"
BASE COUNT         7000 a         2250 c         3188 g         6051 t
ORIGIN      
        1 aaaaatgttt attctaaaat aaattatttt ataagcttac ttatatttaa taagagggct
       61 ttttttattg gaagatttat gttatattga gataaattta ttatgataca aaaaataatg
      121 ttaggataaa tttaaatctt tttctaaaaa tgtataagtg taattcttaa agaaaggatt
      181 tttaacaatg gatataaaag agttagttaa aatacaaaga aaatattgta atacaggtaa
      241 aacaagagat atatcgtttc gtatagaaca gttgaagaaa cttaagtcag ttgtatcaca
      301 aaatgaagaa aaaatacttt tagcgctaaa gagagattta gataaatcgg attttgaagg
      361 ttttatgaca gaggtgggca tgttttatag tgaattaaat tttgctataa agaatataag
      421 aaaatggagc aaaataaaaa gagttaaatc aagtatggtt aattttccat ctatatctaa
      481 agttgttcca caaccttatg gtgttactct cataatgtcg ccttggaatt atccattcca
      541 attagcactt ataccattag tttggtcatt agcagcagga aattgtgtta tactaaagcc
      601 ttctgaatat tctgtatcaa catctagtat agttaaagat ataattgaaa atgctttttc
      661 aaaagaatat gttgcagttg ttcaaggaag tcaagaagaa agtgaaaaat tactattaga
      721 aagatttgat tatatatttt ttacaggaag cactaatgtt ggcaaaatag tcatgaaatc
      781 agcatcagag catttgactc ctatcacttt agaacttgga gggaaaagcc cttgtatcat
      841 tttaaaagat gccaatatag accttactgc taaaagactt acttggggaa aactaataaa
      901 tgcaggacaa acttgtgtgg ctccagatta tgttttagtg catgaagata aaaaaaatga
      961 gttaatagag aaaataaaat attatactaa taagtatttt ggagataatc cgtctgctaa
     1021 tgagcaattt cctaaaatca taaatcagag acattttagt cgtatattat ctcttattga
     1081 taaggataag gtaatctatg gtggaaatta taataaggag acattaaaga tagagccaac
     1141 tatagtagac aatgttaatt gggatgataa tataatgaag gaagaaatat ttgggcctat
     1201 atttccaatt ttaacatata aagatttgga tgaagtaata caaaaaatta tacaaatgcc
     1261 aaatccatta gccctttata tttttacaaa aaataagtac ctagaaaata aaattttaga
     1321 aatgattcca gcgggtggat gttgtatcaa tgacacagtt actcatatag ctacgaatta
     1381 cttacctttt ggtggtattg gtgaaagtgg tatgggatca tatcatggaa aagctggatt
     1441 cgatacattt actcattata agagtgtgtt aaaaaaatta aatttggatg ttcctattag
     1501 atatgctcca tatgataata aattaattaa gattcttaaa aagataatgt aggttatatt
     1561 gtagaaaaat aaatataaat aaaattgaat atttgtattc tgaatatatt ttatttttaa
     1621 tttggcatat ttgttataaa agtggtgatt ttatgataaa atttaattat atagaaataa
     1681 ggaggaaaaa atgaaaatta attggaatac aaaatacaca acaatagcaa tttatacctt
     1741 tataatagcg gctagtagta ttatatttta tctagtatca tcacaaattg atgtattttc
     1801 taataattta gacgctatgt ttacaacatt acaacctttt ataataggat ttgctattgc
     1861 atatcttctt aattttattt taaagtttta tgaagacaga atttttataa aaagtgagaa
     1921 attgaaaaaa ctaaaacaaa gttcaaaaag aggtttgggt attttattaa catatgcaac
     1981 agccatgttg atactttact tatttatgta tttcgtattg ccacaagtaa ttgagagtat
     2041 tgttggacta gcaaatgata tacctatgta tgtaaacaat gcaactaagc ttattgataa
     2101 attaatgact gatttaaatc ttgatgagca atactttaat ttggcagttg ataaatggaa
     2161 tgagtttgtt acatatatta taaagtttgt aacagattta atcccaatat taggaagtat
     2221 gttaaagaat gtagcctcta gtatatggaa tattgtatta ggattaattg tttcagtata
     2281 cctattaatt gataaagaaa aattctatgg attaagtaaa aaaataactt atgcagtgtt
     2341 tacagaaaaa caagctgcta gaattttaga attaactcac agaagtaact atacatttgg
     2401 aagattctta ggtgggaaaa tattagattc attcattgta ggtattctaa catttgtaat
     2461 acttacatta gttaaaatgc catatacatt actaatatca gttataattg gtattacaaa
     2521 tattattcct ttctttggac ctttatttgg agcaatacca tctacactaa tagtattgtt
     2581 tgtatctcct ataaaagctt tctggttatt gttaattata ttaataattc aacagataga
     2641 tggaaatatt atagggccta aaatacttgg agattcaatt gggatatctg cattttggat
     2701 tttgttctcc ttattggttg caggaaaatt attaggtttt attggaatgg ttataggagt
     2761 tccaatgttt gcagtaattt attctataat taaggatatt gttgaaggta aattagacaa
     2821 aaagggatta ccgacagata cttccgatta tatgtaacta aaaaatgtta tgtataatta
     2881 aaaatatata aattaagtaa tatttaaagc actgagaaag tcaaactctt ggtgcttttt
     2941 agatataaaa ttattataga agaggtgttt tttgtggcat ttatagaatg taaatttaaa
     3001 tctaagtata ctggaggaga aactgatata attgtaattt tacctgtagc aaatggaaaa
     3061 gaacttttta gtggtaaaga gatatatgat tttgaaggta aatttaaaac attatactta
     3121 tttcatggtt tagaagatga ccaaacctca tggattagat atactaatgt agagcattat
     3181 gctaaaaata aaaatatagc tctagtgata ccaagagtag aaactagttt ttacacagat
     3241 atggtatatg gacaccaata ttttactttt gtaagtgagg aattacctaa atttgttaga
     3301 gctatatttc ctttatcaga taaaagggag gacaactttg tagctggcat gtctatggga
     3361 ggatatggag ctttaaagtt agcactttca aaaccaaaag agttttcggc agttgcaagt
     3421 ttttctggtt cacctgatat gattcatgag ttagaaaatc aacaaataga aaccaatgca
     3481 gatattctat ttcatgattt tggtagacca gaagatgcta gaaatagtga gaatgattta
     3541 ctttacatac ttaagaacct taaagagaga aatgaagaca ttcctaaaat ttatcaattt
     3601 tgtggagata gagatttttt atataaaaat aatcagatgt ttaaaaaata tgcagagtct
     3661 ttacatgtag atgttgaata tgaagagtgt gtaggagggc atgaatggcg tctttggaat
     3721 ttatggataa agaaatttat agatataatt gggtagtatg cttatggtat taacagagat
     3781 actataaaat atctttaaat atgataaaga ctgaaatcat aaaatcaaat catgatttta
     3841 aaatttcagt ctctaagaat ttaataaaat tttattaagt tcttacacga gttattattg
     3901 gagtaaaatt gtcaactata tctatatagt atttactcaa ttcttctaaa atctcttcgc
     3961 ataaatcagt actatgacca agattaataa gaaactgttt tgtaaaatgc tttattacag
     4021 cttgagaaga tatatcatac ttgtgtgaag tataaactct aattatttca ggtttcatag
     4081 caagtatacc aatacaagaa agtattaact catcatgttc tatagattct ttatcattaa
     4141 catatctttt catgaccatt tcggttacac ttctttgata gcttaaaatg gcttcttcat
     4201 aagctatatc caaataaaaa tttctatatg cttcattttt tagaaggaaa caaaggtcag
     4261 cattcatagc aagaaagaac atcatcatag taccttcttc atggtagtta tagattaaag
     4321 attgcatttc atttcttata tcttcatata ttatagctcc tatttcgctt ttacttgaaa
     4381 agtaatagct tattagacct aaatttatat tagctctttc tgcaatttgc ctagtcgttg
     4441 tcttatgata accttgctca aaaaataatt ctctagcagc acttataata ctagcttttg
     4501 tttctttgcc tttttctgat tttgacatat ttactgtgta tacctctctt ttcaatcttt
     4561 ttttgaaaat ttgctaacta taaatattgt aatagaacca cctattatag gaaccaaaat
     4621 tctatcaaat acagtaagaa taccaatcat atttaataat gccagtataa cacttactat
     4681 aacagcatat atagctcctt tttttgtagc atctttccag caaagaccaa ttacaaatgg
     4741 tgcaaatatt acacctgtac atagtggagc tggccaaaac aataattcta ataaactatc
     4801 aaatttaagt gctatgattg cacctattat agctgttaga gctgatgcaa tttgagaata
     4861 ctttagagta ttttttaaat cattaaattc tatattaggg tgtaacaatt tgttataaat
     4921 atctcttgat atgatagcac tcattgaggt cattaatgaa tcggcagtag acattatggc
     4981 agccaatata gcagataaga acaatacaga gattaaaggt ggcaaagtag tcgatatcat
     5041 ttgaggtaaa acctgaacag atgatatgtt tggaaataaa ctaccaccaa tagctccaat
     5101 tattcctata gggaaagcaa gaaataatag tataaatcct gcaattacac tacttttaac
     5161 tgcattttta gcattatcag cacattgata tcttaaaaaa ctttcataag aaaatattga
     5221 gcctaaaact aatggtaaaa ctgtatacat aaaatcacta atcgatttat ttcctggaaa
     5281 gaatggttct tttaccattg attgtaacat aagtaaaggg ttgtcgccaa ctaattttaa
     5341 gcttatataa gctactattg gcaaaaacac tatacaaata gcaagttgaa taaatgctga
     5401 tactgcaact ccccacattc ctgatgtata agtatatata atgatgacta taactcctat
     5461 aattacagct atatcataag gaatgccaaa tgcagtagac acagaaccaa aagagattaa
     5521 ttgactaccc ataatggcta gtatagatat tggtgttaca agactttgat aagctcttaa
     5581 aaatatacta tgaccatatc tagtttcaat ataatcagca ggtgttatat cgcaagataa
     5641 gcttcttaat tttcctgcaa aaaatgcgtt tataaagcag gctaaagctg cacctatagt
     5701 agaatacatt ccaacccaaa gccctctttc agctccccat tgaactccac caactaagca
     5761 ccctcctcca aaagatgtag cagcaatggt tgcagctagt ataggtaaag gaagatttct
     5821 acctgcaatt aggaaatcgg atgctgtttt aacttttttt gaaaaataaa aaccaatcca
     5881 tatgtatatt gcacacgtaa caattaaaag taacatagtc tcttctcctt tataaaatat
     5941 tttaataaaa tatattttaa atatatttta tataaaagta gtacgatatt tgttttattg
     6001 gctttagatt aatatagcta taataattta tgatattaga tagaaattac ataaaaatag
     6061 aaaattgaaa attctgaaat ttttgcatat tgatatttat cgcgtagatt ctttttttgt
     6121 aattgtaaca tgcttttatt tttaaatcaa tagtacatgt ataaaaataa aaggaattta
     6181 aataagaaaa agatattttt aatatctgga tgtagtataa aaatatcttg aataaattac
     6241 tcttgaataa aattgaaaaa attaaaatca actagaaata cgataaaata atttaaaata
     6301 tgtattttga tgacatttaa aaacaataaa ataataaaaa acaaatctaa attaagctta
     6361 aatggctttt tagttaattt taatagtaaa aattaaactt aaaaaatatt tgacaattat
     6421 gaattttgag aatataattg aattaaaatt agacacatat aaaaatatac tctgaatcaa
     6481 gaaggatata tttaagattc aaaatatata ttattatacg tgaataataa aaaagtaagc
     6541 aaattttata agggaggggt agatgtgagt agtcaagaaa gcaaaccaaa aggaaacttt
     6601 atatcaaatt ttagagatga tatgagagac cctaagaaaa gagcaatata cacatattca
     6661 agttctataa acctagcgta caattttata ggttactttg tatcaactgt tacaaatatc
     6721 tttttaaccg atgtggcaaa acttggtgta attatggcag gtggtatgca aatcatacaa
     6781 tctttcataa aaattgctat agctcctata acaggtacag tatttgacaa acaaccattc
     6841 aaaaaaggaa aatattatcc atggcttaga tatgcaccag cagcactagc attaacgtat
     6901 ggaatatttt tcatagttcc attaatgaaa ataagtccag cattactttt acctttgatg
     6961 acagctttat tattaatagc atcttgtgct cagcaagtag ttttcactat aatttatgct
     7021 atttaccctg tagttgctaa gacacctaga gacagagtta taggttcaac tactacgaat
     7081 atattcaaag aagttggtaa attcttagta ggtttttcat atccgttatt attagtttta
     7141 tttgcagatg tattaggtgg agagggaatg ggttatttag gtacctattt attctttgca
     7201 gcaggatgtc ttttagtatt ttggttttct tcaacagaga taataaaatc tggagcagaa
     7261 gaagaagttc ttcaaaaaga agagaagcca aagattaaag caagtgaaat ggcaaaagct
     7321 ttattcacta atgtaccact tatgatggca ttttgtctag aatttcttat atgtataaga
     7381 tctataggat taggaccttt ggctccatat tattttaaat atgtaatagg ggatgaacgt
     7441 ggattagcag tatttttatc aatcatgcct ttggtaagtg tagcgttcat gttttttgca
     7501 ccagtattca taaaaatctg tagagagcaa aaactagctt cgataatttc tttttctata
     7561 tgtgcattat gccacttatt ggttgcatgg actccttggg ggaaaactac tttaggagta
     7621 actatgttgc tggcaattgg tggaggtttt tcaaatgtag tatcaataat aaatcttaat
     7681 ttttttgcag gttcttgtga ttatggtcac tggaaatcag gaaaagactt acctggttta
     7741 agtatgtcat tatatccagt tgcaatacaa gtaggagtat tacttgcaac tactataaga
     7801 acagttttaa tgaactcaat gggatatcaa gctgatatgg tagtcacaga agcagttaaa
     7861 tcaggtttta taaatatgat ttcttattct atggcaatac ctttaataat agctgttgtt
     7921 atagcaatac tatatccagt aagtgacaag aaattaaatc aaataagaga agaactaaat
     7981 caaagaaatg cttagttaaa agaaagttaa gtagatgact aatttaagtt attaaagtaa
     8041 gtagacgcaa tagcgtttat ataaatgaaa atacatatac agagggggct tttaaaaatg
     8101 atagaaaaat tattgagaaa tacagtaaaa gaattacatc aatatgttcc aggtgagcca
     8161 atagaaaaag ttaaggaaaa gtatggtgta aaagaaataa taaaattagc ttcaaatgaa
     8221 aatcctctag gtccatcacc aaaagcaatg gaagctatga ttgaaatgtt aaaacaaggt
     8281 caactttatc cagaaccaga agctaacgaa cttagaagaa aattagctga aaagctagac
     8341 ttaaaaccag aaaattttat tgttgctaat ggtgctgata acgttatcac actaataggt
     8401 gaggctttta taaatagagg agatgaagtt atatattgta acccaacatt tccatcatat
     8461 agaactgcaa ctataaaaaa tgaagggata ccagtagaag tacctctaac agaagattat
     8521 aagtatgatt tacaaggtat attggataaa attacagata aaacaaaatt aatatgtgta
     8581 tgcaacccta ataatccaac aggaacaatt gtagatgata aagagttaga agaattttta
     8641 aagaaagttc ctgaaaatat tataactata ttggatgaag catatataga atttttaaca
     8701 gttcctaatt atgtggatgg acttaagtat gtaagggaaa attacaatgt tatagtaaca
     8761 agaactttct ctaaaatata tggtcttgca gggcttagag taggatatgg tattgctaaa
     8821 gatgagataa taagaactct atttacagta aaagagcctt tctctgcaaa ccgtgttgct
     8881 ataagtggag caacagcagc acttgatgat gaagagttta taaaagaaac ctatgaatta
     8941 aatagagttg gtatggctta ctttaaagaa gagtttacaa aaatgggatt tgatgttgta
     9001 gattcacaat caaatttcct ttatgtagat atgaaaacag atatacctaa gttatttgaa
     9061 gacttgaaaa aaagagggtt tgtaataaga ccaagtgcta cacatgcacg tgtaagtatt
     9121 ggaactatgg aagaaaataa gaaatttgta gcagttttaa aggaaatatt aaatattaaa
     9181 gaggagccaa gtattggata atagatagaa atattatgaa acggggtgta gatatgaact
     9241 taaaaataaa cgcagagaga tgtaaaagtt gtgaatactg cgttatatct tgtaaaaaag
     9301 gagcattaaa aatatcaagt aagataaata aagaaggtta tgctcatgta gaagttgatg
     9361 agagtaaatg catactttgt ggaatttgct atcaagtgtg tccagataat gtttttgaaa
     9421 taattaaaga agaagcatgt caagaggctt agaaggagtt gggataatat gaaaaaaatg
     9481 tggaagggga accacgccat tgcagaagca gccctaagag gtggttgtga attttatgca
     9541 ggatatccaa ttacaccgca aacagaggta atggaatttt tatcacatag aatgtctgaa
     9601 cttggaagaa cttttataca atctgaaaat gagatggctg ctatatatat ggtatatgga
     9661 gcatatgcaa gtggaatgag gtctatgacg agttcttcag gaccaggaat atctttaaaa
     9721 caagaaggga tttcttactt atgtgcaaat cattaccctt gtgtaatcgt aaatgtacag
     9781 agatggggac ctggtcttgg ttcattggat tcagcacaga cagactacct aagagatact
     9841 agagggggag gaaatggaga ttatcatttg atagtttatg caccaaacag tattcaagag
     9901 actgtagatt tgatgtataa ctcatttgat gtagcggaaa aatacagagt acctgttgaa
     9961 attttgacgg aagcagcttt aggtcaaatg atggaaccag tagaatttcc agagtttaag
    10021 aagagagaag aagatttagg ttggacttac gatggaagta atagagacca tgcaaaggtt
    10081 gcagataacc aaaagccaac tttttgtatg gagaaacgta tgagaataag tgaaaatgaa
    10141 caacaatggg aagattatca aatagaagat gcagaatatg tgtttgtagc gttcggtata
    10201 ccatcaagaa caactatgaa tgctgttaga agacttagag aacaaggtga aaaagttgga
    10261 ataataagac caataacagt ttggcctttc ccatataaag catttgaaaa ggtaagcaaa
    10321 gatgtaaaag gatttatatc agtagaatca acagacacag gtcaattagt agaagatgtt
    10381 gctttagcat caaagaaagt atgcaaagaa aatgtacctg tgtatggtct atttagtgga
    10441 aatcatatac caaaaacatt gcaagtaatg gacacttata gcaaaataaa atctggcgaa
    10501 atcaaggagg tgttctaata tggctacaga aaaattaaca cctagcgttg tttcaatacc
    10561 aaacaagttt tgcccaggtt gtggtcatgg aatagtaaac cgtataatag cagaggtaat
    10621 agaagagcat ggatatgaaa aaaatcatgt gttgacatta ggagttggtt gtgtttgtaa
    10681 tatgaatttt agctggaatg gtgataagat gcaaactgca catggtagag cttcatctac
    10741 agctatagga gttaaagtag cacttccaga tacactagtg atgacttatc aaggagatgg
    10801 agatgcttat gtcataggtc tttctgaaac tttaaataca gcatatagaa atcataatgt
    10861 gactgtattt gtaataaata ataataattt tgcaatgact ggtggacaaa tgtcatggac
    10921 cactatgcca ggtcaagtta caacaacaag tgcaaatgga agagatataa gtactactgg
    10981 tagtccacta aaagttcctg agatggtggc aaacttcttt gatgtagcgt atgtagcaag
    11041 aggctcagtt catagcccaa aagaaataat taatctaaaa aaatatataa aaaatgcaat
    11101 agaagctcaa ttaaatggag agggatattc cttagttgaa atattagcgc cttgtccaac
    11161 taactggagt gtatcactag agaaatctat caaatggatg gaagaagaaa tagtgccata
    11221 ttatactctt ggcgaattta agcaaaggga tggtgaatag ttatggaaaa agagttagta
    11281 tttgcagggt ttggtggtca aggagtttta actcttggtt tgattgtagc tgagtcagca
    11341 cttgaattgg gtaaacaagt aacttggatg cctgcatatg gtccaactat gagaggtgga
    11401 aaagcctata gtgtagtaaa gttttctgat gagagtatag gtggacctga tatggaagaa
    11461 atagatgttt tagtagcaat gaataaacca tctttagatt atataaattt agtcaaggaa
    11521 ggtggaacag tagttattaa tacatctgca atagatgaga atatagcatt aagagaagat
    11581 ataagtgtag ttaaaataaa ttgtcaagaa ttagctcaaa aagtaaataa tccaaaagca
    11641 gcaaatatag tagtacttgg agcattaatt gctaagactg gattgtttga aaaagaatta
    11701 gctttaaaaa ctatgtgtga tttctttgag gaaaaaggaa aaggaaagtt taatattcaa
    11761 aatgaagcag cattcatgga aggctataat aacgccattt gataaattac ccttatctta
    11821 agtgatttat ctcgagattt aataatcttt agtgccaaga gaaatttcat cttgagataa
    11881 aattcactta aaatcacaac tataaacata taggagcgtg aatttatgag tttggaaaaa
    11941 cttatcagac caaaaagtat tgctatagta ggagtgactg ataaacttgg ttttgggaga
    12001 agtgcagcac ttagtatagt aaaaagtaag gacacagata gagtatatta tgttaatcct
    12061 aaaagagaag aattgtttgg gagaaaatgt tataaaacga tacaagaagt tccagagata
    12121 gtagactgtg tagtagtatg tacacctaga aatgtagttc catctgtatt aaaagattct
    12181 gcagagttag gaacaaaggc agcagtagta tatgccagtg gatttgcaga ggaaggtaca
    12241 gaggaaggta cagatttaga aaatcaactt atagaaatat caaatacata cgatatgaaa
    12301 atattgggtc caaactgcat gggattgcta aattgtatag ataaagttaa tttgtgggca
    12361 ggaggttcta aatgggattt agatactaaa aagcctggta taggaattgt agggcagagt
    12421 ggatttataa ctgctgagat tgtttcaagt gattatttta atatatctta tggtttttca
    12481 acaggaaatg gaaacatagt tacactagaa gatgcagtag atttccttgt agatgataat
    12541 tatgcatcag taatagcaat ctaccttgaa ggtttaaaaa atcctcaaaa atttatagat
    12601 gctttaaaaa gagcagcaca aaaaagaaag ccagtgataa tcttgaaatc aggaaggtca
    12661 gaaaaaggag ctatttctgc tgcttctcat acaggaaatt tagctggttc aagtaaggct
    12721 tttgaaagta tttttgaaaa atatggagta atttctgtag aaaatttaga acaatttatg
    12781 tgcttagcac aagcttttag tgttctagat ggcaatttac ctacaaattc taattttgca
    12841 gcaataaact tctcaggagg agaaaatacg atttgtgcgg atttagcaga agaaaatggt
    12901 gtagaacttg cagagatatc ttctgaaact aaagaagaaa tgaaaaaata tcttccggga
    12961 tttgcaacac ctaaaaatcc actagatgct acaacagcat tattccacga aaaggatatg
    13021 atagtaggtc tattacatac ctttgaaaag gacagtagtg ttggatttac tatggtaggt
    13081 gcaaatatac gagatgaaga aaatgaaatg cacgaaacac tatgtcaagc tgtaagtgaa
    13141 gctagagaac aaggattgaa aaagcctgtg tttgcagttc ctacacttga aggaaataga
    13201 tatttagatt ataggaatag attagaatac aatcaagtgc ctataatgtc atcagttgga
    13261 actacattta cttgttttaa taaaatggct aagtttatag attatgatta ttcaaaaagg
    13321 actttagaat ttaaagctgt taagaaaaga gagtcaaata atgtagtagc attatcagaa
    13381 ttagatagta agattgagat gaagaaatat ggagtaccag ttccaggaca aggtaatgca
    13441 aaaagtattg atgaattaga tgaaatctta aaatatatta aatatcctgt tgttttaaaa
    13501 ataaattctt ccgaaatctt gcacaaatct gatgttggag gagtcaaaat aggaattaag
    13561 aatagagatg aggcagtaga tgcatataat gagattttaa ctaatgtaaa aaaagcaaaa
    13621 ccggatgcta atattgatgg tatattagtt caagaaatgg tagaaagtgg tatagaaatt
    13681 attattggta taactaatga tgaccaattt ggaccaatgt tgctagttgg gttaggagga
    13741 gtctttgtag aagtatttaa agatactaca ttatatccat tacctataaa tcacgatgaa
    13801 gctattatga tgcttaaaaa actaaaatca tttaagcttt taaatggtta tagaggaagt
    13861 gagccttgcg atattgatgc tcttgcagat atgatggtca aattaggaaa atatgcttac
    13921 gaaaataagg atgaagtcaa ggaaatagac ttaaatccag tgtttgtata tcctaaagga
    13981 aaaggcgtat gtgcagtaga tgccttaata gttaaatata aatagtttat aagttttggt
    14041 acttaaccat aattaaagca ctgactgaaa atcacatttt gtaagttaaa tagtgtgatt
    14101 ttcggtcacc ccaaatttac tttaaaaaaa ttttaaagta aaagtatagt tttaagtttc
    14161 attgtgaaat agtactgtaa aaacaataaa atactacata taaaacgaat aataaaaggt
    14221 gttttaatga taagaatcaa ttttgacttt cactaaaaca ccttctttat tatatgaaaa
    14281 atttttaaat gttatattgt cacaggtggg tttgttggag ctgaatatgt acgattagca
    14341 aattcttggt caacaaagaa tagaccacct tctccagcaa gtttaacacg ttttatattg
    14401 tcttcacaat ttgtttcttc ttctgcttgt tcagatataa accaaagtaa gaattgagaa
    14461 gttctaaagt ctgactcaga gtttgctaca gctgctaaat tgtttattaa agctgtaacc
    14521 tttttttcat gagctaacgt acgctcaaga atatccattg cagaagtaaa gtctgaatct
    14581 ggcattggga tagcatcaag ctgaaccttt cctcctacat tgtgtaagta tttgtaaaaa
    14641 aacatagcgt ggtctgtctc ttctttatat tgaacataaa accaattaga aaatccttta
    14701 agtccttctg cttcaaggta agaagacata gaaagataaa gatatgcaga atataattca
    14761 tggttaatct gctcattaag taatttttcc attttagctg aaatcatttt tatacctcca
    14821 aattttaaat gctcttataa aatataataa tattaaatgg aaaaatattc aataaaagtg
    14881 tttaaatatt ttacatattt aataaaaatt tatactacca ttaaaaaagt tataacttac
    14941 aatggctata gagccgatta atggtgcatt tgctccaaaa tttgaatgtc ttacacttat
    15001 ttttttatat ttagaaaaac ttgctgaaac agtcaatttt tttaataatg tattttctat
    15061 aaatgtacca ggaatattaa agtcatagcc tattattata gaactacaat caatcaacgt
    15121 aagtgtatta accaatgcat atgatacgta tgtacaaaat tcatcaagca aaaacatagc
    15181 gagactatct ttattatttg cctcatctat taatttgact aaagaaaaat ctttacagtt
    15241 aattaacggg gaattaggat aaatatggct caatgattca attcttttaa ttagattact
    15301 gacacttgta taataatcta aacatccatt gttaccacaa ctgcacaatg gtccactaaa
    15361 attaattgat gtatgtccta tctctccact ttgccctaga ttgccagtat gaagtttatt
    15421 ttctagaact agaccagctc caataccatt cattatgtgt aggtatatat aatttgatat
    15481 atttttacct aagccataca ttttttctgc caatgcgccc gaattagcat cgtttattaa
    15541 aaatattggc aaatcagaca gttcttttat aaaagctact atatttaggt ttgatacatt
    15601 tccaaagtct gggggattta gtatggtacc atttatatca tctactggac ctattgaaga
    15661 aataccaatt ccaattatat ctctcttgtt gattttcatt attttggtaa acattctttt
    15721 tattatatct tttaagacat catttgatag acatttaggg taagtttcag aaagttgttt
    15781 tacaatatta ccttttaaat ctgaaataat taccttacat atatttcttc ttattaggat
    15841 tccacaaata caaggtgata tattagaaat atctaataaa ataggtttac gacctgatga
    15901 atgtgcattt aaagttgatg actctatttc cttaatcatg ttttcattaa taagttcagt
    15961 tactagattt gataaagtca ttttagtaag tcctgtagac ttagctaaat caaccctgga
    16021 cataggtgta tttgtagaaa gtaatttcaa tattattaac ttatttttaa tttttacatc
    16081 ttttatgtta gaaccttgtt tgttactcat cttaaatctc cttaaataaa gttaagtgtt
    16141 ttatttattt tcaagtatgc tatcacctta taatagagca acattttttt acttgaaaat
    16201 atcattaatt agaatatatc acattatata tcataaaaca caaaaaaatg ttaggcttaa
    16261 attaaaaatt aaaagttaaa agttaaaagt agattgagat ataaatagta gatactcaat
    16321 taaaaaagat aatgataaca atatctgctt ttataaagaa accaccattt caagcattac
    16381 tttaagtagt taagttgatg gtagttcata tataataact tgataaaagg tttacttaat
    16441 aataaataga ttatgcaaat ctacaatcta tttattgtat acaataaaat ttcattttgt
    16501 aaaacagtga attaaatatg aaatacagag atagattttt attacaaatt gttaccaaat
    16561 atagatatat tagaacagaa atctagtaaa ttgtaactaa aatgtaaatt tttcctttaa
    16621 tctatacaat attatggaaa ctttttgtaa tctatgatat aataacaaat atataattat
    16681 taaattataa taaatgacta tagcgtagat gtattactat gctatagtca tttttgaatt
    16741 aaacttgata tatcaagggg gaagaatatg agaacaatta gaagagtttt agcattaggc
    16801 ttaacattag ctatatttct aataaatgta cctaatgtag atgcattaac atcagataca
    16861 attaaaggta ataatatata tgagacggct ggatcaatag cagataaaaa gagttatgat
    16921 acagcaatta tggtaaatat ggataattct atagcagatg gtctttcagc aagtggtctt
    16981 gcaggtgctg ttgatgctcc aattttgctt gcacaaaaaa ataagatacc tagtgaaaca
    17041 aaacaaagac taaaaaatgt taaaaaaata tacataatag gtaaagaatt atcaataagt
    17101 aaatcagtag aaaacgaact gaaaaataca ggagcacagg taactagatt gggtggagat
    17161 gacagaataa agactagtta tagtgttgct aaagaggtaa atggtatcaa aaaagttgat
    17221 gaagtgatac taactaatgc atataaagga gaagcagata caataagtgc tgcacctgta
    17281 tcagtaagag atatagctcc tatagtactt acagatggaa agagtgtacc tttttcaaca
    17341 agtaatgtaa aaacatatgc agtaggtgga agtatatcca tgagcactag tttagttaat
    17401 aaaactaatg ctaaaagact tggcggttca gatagatatg atacaaataa gaaagttata
    17461 aaagagtttt atccagatgc atcagaattt tatttaagtg atggatatga tttagtaaat
    17521 gcacttacag gttcaacaat tgctaaggaa aatccaattg tattggtgtc agaaagtagt
    17581 gataagtcta tattagcagg agcagataag attactagat taggttcaat aagtgatagt
    17641 gtgtataata agtgcgtttc tgttgcacaa aataatggag attcgtctac aaaaggtgat
    17701 tcacctatga aaaatgagac aagcatatta ggtcagccaa cagctagttt agaagcttgt
    17761 ttaaaatggg ccaaatctaa aaaagctaat gatttattta tagagttaat accaatatta
    17821 tatgatactg ctgttcagga aggtgtaaat cctgttttgg cagtagctca atctgcaaaa
    17881 gaaactggtt tttgtaattt tggtggagtg ttagatgcat catttaagaa tccttgtgga
    17941 cttaaaactt ctgtaggagg ttctgatact gataagaatg ctcattcaag atttgatact
    18001 tgggaagaag gaatattagc tcaaattcaa catttatgct tatatgcagg acaagatggt
    18061 tatccacttt caaatccagt agaccctaga catgaaaagt ctttatttgg taaagctaag
    18121 acagttgaaa gtctatctaa taattgggct ggaggtcaat atggacaaga tttagtaaga
    18181 atgatgggag aaattgaggc aacaaaataa ttgaagatta tcaaatatcc aataaataag
    18241 gattgattat tatgaaaaag agtaatattt ttagtattat atttcctaca gtcataatgg
    18301 tgctaatgtt tttaactttt gttacagata tatttaatct tgcagatgtt caatctaaaa
    18361 gtatttttgt tgtaggaatg gttttgattt ttccaatttc ttttttaata caaggaataa
    18421 tttgtgtatt aaataagact agttggattt tatctcttat tgtgtcttta ttaacttata
    18481 tagtattaa
//
LOCUS       DAFNNB010000069        18443 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0070, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000069 DAFNNB010000000
VERSION     DAFNNB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0070"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            118..1335
                     /locus_tag="L3M60_002018"
     CDS             118..1335
                     /locus_tag="L3M60_002018"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HBN6226683.1"
                     /translation="MMNVTLTDVKEARETIKNIVKRTDLLESVKLSEKTGANVFYKCE
                     NLQKTGSFKLRGACNKIASLTDEEKANGVIASSAGNHAQGVALGAKMTGIKSTIVMPA
                     TAPLAKVSATKSYGAEVVLNGAVYDDAYAKAVEIQKETGATFLHPFNDRYVIAGQGTI
                     SLEIFEQLDNKVDTILCPVGGGGIISGVAVAAKALNPNVKVVGVQTANIPSMKESIKN
                     GKVTTAFNDTTIADGIAVKTPGDLTFEIINELVDEIVVVEETEIAESILFMMESQKIV
                     SEGAGAVCTAAILSGKYVPAKDENVVCIISGGNIDINTLYRIIGVALAKEGRRYSFST
                     IMEDKPGNFAELTRIISENGGNILSANQGKLSAGEALGKQSAEFILETIDYDHIARIK
                     KAIEEKGFKIIEL"
     gene            1430..1810
                     /locus_tag="L3M60_002019"
     CDS             1430..1810
                     /locus_tag="L3M60_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBN6226684.1"
                     /translation="MKHEVIHTNDAPAALGPYSQAIKAGNLLFVSGQVPLVPETMEVV
                     EGDVQAQTAQSLKNLKAILAESGADFSNVVKTTVFIKDMNEFGAINEVYAEYFGENKP
                     ARACVEVARLPKDVKVEIEVIAVL"
     gene            2096..2578
                     /locus_tag="L3M60_002020"
     CDS             2096..2578
                     /locus_tag="L3M60_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBN6226685.1"
                     /translation="MLNFFKKNKSYKLHAVVSGNCINIEKVNDSVFSKRLMGDGVAII
                     PNSNVVVAPCNGKVTVLTESKHAFGMVSDEGVEILVHIGIDTVNLQGDGFKNEVSQGD
                     NVKKGSPIISFEREKINSQGIDCTIIMVVLNHSEFSEINCMVENEVVAGQDTVIEIMK
                     "
     gene            2599..3414
                     /locus_tag="L3M60_002021"
     CDS             2599..3414
                     /locus_tag="L3M60_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6226686.1"
                     /translation="MRVVKIFNNNALSTVTNDRKEAILLGLGIGFNKRPGDKANEDKI
                     EKIYYVQDHMQTKFLELLKNVSPEVMDASQQIISLIPGNEGKFNNKGILSLVEHISFA
                     IDRMKNNVFLPNLMLLDIKMMYSKEFELGVKALEVIYRVCHIHLPEDEAGYIALHFVN
                     LQSNDNLAYDTLKFVKGSINLIKECYGLELDESSLSTLRFRTHLKFLAQRIFKNEICQ
                     DDKMIEMYDYLINNHPKNKEYLEKLNIYIEKEFRYKLDKPEKIYLLLHLTKIL"
     gene            3467..5017
                     /locus_tag="L3M60_002022"
     CDS             3467..5017
                     /locus_tag="L3M60_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBN6226687.1"
                     /translation="MKDKLLGGLETFAKAMVQPLMYLSSAGILMVVGVLLTNDMLLGV
                     FPFFKWEPIQIFGSLIYNCIMAIINNLAVIFAIGIPAALAKKEKHKAALIGFMSYIIY
                     LTASNTMLTSLGQIAKESKMLGLIGTGQATILGIQVVDTSVFGGIILGCIAGYVFNRT
                     SNKTFKGALQIYSGANFSFICMFFVSILIGVVSNFIWPLAQQGIASLANVIKDTGTFG
                     LFLYGFLERILVPTGLHHLIYTPFQFSDLGGVLKVGVDTVSGAYAIVMTEMNLPVERF
                     SDSIYYMATGFTKMFGYIGIGAAFIYTAYKQNKKKVKATIIPLIITAVLASVTEPLDF
                     MFVFCAPLLFFVHAVIAGLFIALLKIFDVTALCGGNLLVSIIMNTTVGVEKTHWPVMM
                     ILGLAQIIVYFVVFSFLIKKFNYKTPGREDESTATGEGQSKQLNLDTGIENIIDGLGG
                     KENINTVENCITRLRVNVKDESKINEDIINLTPNSGIVRKGKDIQIIFGLHVHEVRRA
                     VEDFLEIY"
     gene            5064..6416
                     /locus_tag="L3M60_002023"
     CDS             5064..6416
                     /locus_tag="L3M60_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HBN6226688.1"
                     /translation="MVITIVGGGSTFTPGIVKSIALREKELELEEIRLFDIDKERQDK
                     VAVVVNWILKEDVKTDVKLTVTNCEKEAYTDATFIFAQMRVGKYEMREQDEKIPLKYG
                     CVGQETCGCGGMAYGLRTIQPMIKVIDDVEKYAKKGYWILNYSNPAAIVSEACRKLRP
                     NARIINICDMPIAIIDMIAGSLNINDVHNIRYDYFGLNHFGWFTSIDYKHRDLMEEIK
                     EYIKENKILLPKSYIENLNKSRSTGNRHAVSSWIHVWESVYTMLENFPKYLPNTYLTY
                     YLLSKETVEHSNINRTRANEVMESREKDLFEGVRHYLETGEISEKAFYAGSHGDWISD
                     LAVSIKNDTRSRFLVITENRGAIPNMPYDAMVEIPAYIGKDGPEVIARGPIPLFQQGL
                     MMQQLNSEKLIVEAYIENSYEKALQAFTLNKTIPSMNVAKEILDEMIEVNKEYWPELR
                     "
     gene            6547..7305
                     /locus_tag="L3M60_002024"
                     /pseudo
     CDS             6547..7305
                     /locus_tag="L3M60_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897697.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7352..8071
                     /locus_tag="L3M60_002025"
     CDS             7352..8071
                     /locus_tag="L3M60_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBN6226689.1"
                     /translation="MNKEYLEETKMLNYNEPQLKLLVSSKKWLELDDFYKIKSIYEFV
                     QNDILFGYNASDILSATQVLNDGMGQCNTKATLLMALLRAVNILCRLHAFDVTKDFQR
                     GATSKLISLLAPKYILHTWVEVFYQDRWIALEGVITDKKYLEAIQKKFSNYGGTFKKY
                     AIATNDLKNTSIDWDGKDTFIQKEAIVYDYGIFPSPDVFFSTHSQHMSKLKNFIYVHL
                     IRKIMTKNVCKARNNYIDKNE"
     gene            complement(8195..9595)
                     /locus_tag="L3M60_002026"
     CDS             complement(8195..9595)
                     /locus_tag="L3M60_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HBN6226690.1"
                     /translation="METQSNNKKGNLIIAIVMTGAFISSLSQTLLSTALPNIMSDFKI
                     TADVGQWLTTIYLLIAGIIVPTTAYLINRFSTRKLFITSMSIFSIGCIIALFSNNFST
                     MLVARVLQAMGSGSLMPLLQVIILYLCPEEKRGAAMSLVGITVGFAPAIGPTLSGWLV
                     DSFGWHSLFLLLSPIAILDVILSFILLKNVGETQKLKLDIPSIILSSLGFGGLLIGFT
                     NQGNYGWTNIATYLPIFIGIISLIFFTLRQLKSREPFLELRVFKNKPFLISTILIMIV
                     YASMMSATLMIPLYVQSVRGFSALSSGSLMLPGAILMVVLNPVAGRHLDKYGPHALSI
                     LGTGCLFLGTLSFAFLGRDTSLLHVSLMYCIRMIGISMVLMPLTTWGIKTLDRELISH
                     ATAINSTLRQISGAIGSAVLITIMTSATKKAHMSSAILSNIHGIDVAFSIAATLAFVG
                     LIVSICFIKRHQIIRS"
     gene            complement(9599..10507)
                     /locus_tag="L3M60_002027"
     CDS             complement(9599..10507)
                     /locus_tag="L3M60_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6226691.1"
                     /translation="MKEGNKVTEKQRLIITIAQKIFDQKGFQNTSISDIVKECKMSKA
                     TFYKHFETKESFICEIINYYDEKFLEIIHSINENNDIPSSEKLNRKIIAVWENIFSRT
                     TINTYIRENFSEVQRKTTSKLQTKSRANLLNEYKLSLFDNYGDKIENIIFDLVFLLDA
                     LIHQFIYIIHVQKREINVYFVAKFTIQILDLVVENMDNLNPLIEKSMFLHKQEDEAYF
                     SLYNKSLFFKTIQDIEELIKTDTSLLENSKLLEATKKLYVEGKNQQYDSLIMDSMIAY
                     LEKEDTLRPKVLLLNSIKNQLKKETL"
     gene            10769..11416
                     /locus_tag="L3M60_002028"
     CDS             10769..11416
                     /locus_tag="L3M60_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBN6226692.1"
                     /translation="MKKKICVAIIFALFIVFVEMFIDSSSEGSKIMTAELSKYRKGVN
                     VLSINNIIVANKKYSLPKDYSPQESSEARNAFYKMNKDAQKSGLNLKAFSTYRSYEYQ
                     DRLFKSYVKEHGEKEANRFSAKPGESEHQTGLAFDIGGNDQSCWANKKFNNTKEAKWL
                     YENAYKYGFILRYPEGKEHITGYMYESWHYRYVGTEHSKNFAMNNLTLEEYLHIN"
     gene            11741..14380
                     /locus_tag="L3M60_002029"
     CDS             11741..14380
                     /locus_tag="L3M60_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBN6226693.1"
                     /translation="MSYKSTYQEVIKNLDSSLSGLSKERAEELLEKNGANELKEADKV
                     PTYKLFLESFKDPLVIILLIAALVQIFLGETVESIIIFAVIIINSVLGVVQTKKAESS
                     LESLKNLSAPNAKVIRDNKKMTIPAKNLVVGDIVFLEAGDYIPADGRLIEAQTLKVVE
                     GMLTGESEPVLKHTEKISEDVSLGDQKNMVFSGSVVVYGRGSFIVTGTGMDTEMGKIA
                     GLLESAENKLTPLQQKIEDFSKKLGVGIVILALLIFIIQVARNYFMVGNGEMTQIILD
                     SFMFSVAVAVAAIPEALSSIVTIVLAVGTNSMAKKQAIIRKLPAVETLGSTSVICTDK
                     TGTLTQNKMTVVDYYMYGTNNDELDKEKVNYSYHAKLLTMVSTLCNDSHITSDGKEVG
                     DPTEVALINYSSKNDLDYDKLRSKYIRINEIPFDSDRKLMSTVNSIYGDYIMFTKGAP
                     DIVFSRCKYALKNGSKVDITDEIIEEYKHKNEEFSNRALRVLAFAIKDVPEDDFVPSI
                     DDEHDMTLVGIMAMIDPPREEVMDAVKEAKSAGIKTVMITGDHKTTAAAIAKEIGIME
                     DGDLALTGKELDALSEEELYEKLENISVYARVSPENKIRIVKAWQHKNNITAMTGDGV
                     NDAPALKQADIGIGMGSGTDVAKDASAMVLVDDNFASIVNAVEVGRTVYSNIIKSITY
                     LFAGNLGAIVSILFAVFAGWSNPFTALQLLFINLVNDSLPAIALGFEKSEKNIMNKPP
                     RHPDESILAGGTLRAVAIRGCIIGIVTIIAQYIGLQVSPELGVAMAFSTLILARIMQT
                     LPARSNNQTVIKLGFFSNKYVIGAVLVCLGLYSLTLIPVLRGIFSIPTTFGLYQLGIC
                     LGLALISSILMELNKLINRSEVK"
     gene            complement(14438..15541)
                     /locus_tag="L3M60_002030"
     CDS             complement(14438..15541)
                     /locus_tag="L3M60_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HBN6226694.1"
                     /translation="MKLKINKAIEMQLKKSYAIESGHSEDVFEIDCGEGINTVSYSNK
                     AVEAFNALKFDMIRGYPHSIALKDNIVDYWKDFLTLDTDRICLADGSIHVIYLLNRLF
                     IEKGDKVLGYSPQFSEYETDIKMHGATYDYVLLKKEDNFKFNEKEFIEKINPEYKVIY
                     IDNPNNPTGQIIPLSSIENIVKEAAKYDIAVMVDEAYGEYMPKENSAVKLLNNYDNVI
                     ALKTFSKGFGLAGLRAGYAVLPEQLVNPIKKISTPYEVSEISRSIAANLLDDVQFIEE
                     LKEKTKDIKNQLLIPWKNLNIAETSDTVSIMTVEHKNKDIDLQQEFAKLKIRVISGSD
                     FTGLDKNFIRFRMPEEKELPEVIKAFQVIDNIE"
     gene            15902..16723
                     /locus_tag="L3M60_002031"
     CDS             15902..16723
                     /locus_tag="L3M60_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HBN6226695.1"
                     /translation="MGYKLIVTDMDGTLLGNNHKVTDENKTALQKVIKNGINVTLATG
                     RAFDSAKCNVDFLKEDMPIIACNGSLIREQNGNIIYSNKIDTTTCLNILDVLDKYDIY
                     YQCNSIDSMLSKKIEGREDRLSVFLGSETEVIIRDDLREEIFKKDILKFVIIEEKNPS
                     ILDEIRKELRKVQGIKITSSWPNNIEVMNEGVDKGNAVKILAEKMNIDREDIIAFGDN
                     YNDIEMIKFAGLGVAMGNAEELIKQEADYVTDTNQDSGVAKAIYKFLELKESLSS"
     misc_binding    16803..17013
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            17100..18416
                     /gene="argH"
                     /locus_tag="L3M60_002032"
     CDS             17100..18416
                     /gene="argH"
                     /locus_tag="L3M60_002032"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012101354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBN6226696.1"
                     /translation="MKLWGGRFRKAENQLMEEFNKSFGYDCILYKKDIEGSVAHVHMQ
                     VKCGLLTEEEGKSITEGLKGILEDVENGKLALDGEYEDIHSFTEINLIQRIGDVGKKL
                     HTARSRNDQVAVDMRLYAKEKANDLVDLISEFKATIKDVADKNPVMMPGYTHLQRAQV
                     VTFKHHLMAYYSMFDRDEKRIKNAIEILDESPLGCGALAGTTHDIDRSITCEQLGFKK
                     VVDNFMDGVSDRDYLLELMSDFSIIMMHLSRLSEELILWSSQEFGFVEIDDLYTTGSS
                     IMPQKKNPDGAELIRGKTGRVYGNLFGLFTVMKGIPLAYNKDMQEDKEGFFDSVHTLE
                     MCIQIMDRMIATLKVNEDKMKQAVKNGFLNATEVADYLVKNNVAFRDAHGIVGSIVIY
                     CEDNKKAIEDLTLEELYKFSDAFKEDIYDFIDYESILNKGIKKNLK"
BASE COUNT         7064 a         2138 c         3263 g         5978 t
ORIGIN      
        1 ttttgtgatt aattaaaaaa ttataaaaat cgtttacatg ttgattttta aaaaaaaaag
       61 atatagaatt atatagaaag taataaacaa aaattggatt tacgggagtg gaatgaaatg
      121 atgaatgtta ccttaacaga tgtgaaagaa gctagagaga ccattaagaa catagtaaaa
      181 agaactgact tacttgaaag tgttaaactt agcgaaaaaa caggagcaaa tgtgttctat
      241 aaatgtgaga atcttcaaaa aacaggttct ttcaaattaa gaggagcttg taacaaaata
      301 gcaagtttga cagatgaaga aaaggcaaat ggagttatag cttcgagtgc agggaatcat
      361 gctcaaggag ttgcattggg ggcaaaaatg actggtataa aatctacaat agtaatgcca
      421 gcaactgcac ctttagcaaa agttagtgca actaaaagtt atggggcaga agtagtttta
      481 aatggtgcgg tttatgatga tgcatatgca aaagctgttg aaatacaaaa agaaacagga
      541 gcaactttct tacatccatt caatgataga tatgttatag caggacaagg aactatatct
      601 cttgaaattt tcgaacaatt agacaataaa gttgatacta tactatgccc agttggtgga
      661 ggaggaatca tctctggtgt tgctgttgca gcaaaagcat taaatcctaa tgtaaaagta
      721 gttggtgttc aaactgcaaa tataccttca atgaaagagt caataaaaaa tggaaaagta
      781 acaacagcat ttaatgatac tacaatagct gatggtatag ctgttaagac tccaggagat
      841 ttaacttttg agataataaa tgaattggtt gatgaaatag tagtggttga agagacagaa
      901 attgcagaat caatattatt tatgatggaa agtcaaaaaa tagtttcaga aggtgctggg
      961 gctgtatgta cagctgctat attaagtggt aagtatgtgc cagcaaaaga tgaaaatgtg
     1021 gtttgtataa tatctggtgg taatatagac ataaatactt tatatagaat aataggtgtt
     1081 gctttagcta aagaaggaag aagatattca tttagtacaa ttatggaaga taagccagga
     1141 aactttgctg agttaactag aataataagt gagaatggtg gaaacatttt aagtgctaat
     1201 caaggtaaat tatcagcagg ggaagctcta ggaaaacaaa gtgcggaatt tatattagag
     1261 acaatagatt atgaccatat agctagaata aagaaagcta tagaagaaaa aggttttaaa
     1321 attatagaat tataataaaa ggtgtatata atatacaaga aaaatagtat aatagtgtat
     1381 gaagatacat tttatatgta ttagattaac gaattaggag gattttaaaa tgaaacatga
     1441 agtaatacat actaatgatg caccagcagc tttaggacca tactctcaag ctataaaagc
     1501 tggaaactta ttatttgtat ctggacaagt tcctcttgta ccagagacta tggaagtagt
     1561 tgaaggagat gtacaagctc aaactgctca atctttaaaa aatcttaaag ctatattagc
     1621 tgaaagtgga gctgattttt caaatgtagt taaaactaca gtatttataa aagatatgaa
     1681 tgaatttgga gctataaacg aagtttatgc tgaatatttt ggagaaaata aaccagcaag
     1741 agcttgtgta gaagttgcta gattaccaaa agacgttaaa gttgaaatag aagtaatagc
     1801 tgtattataa aaatacaata tttttaattt gaaaaggctg aagaactata ttctagtgat
     1861 tcagcctttt tataaaaaat aaatcaaaag cttaaagagc ttgataaata ttgtcgaatt
     1921 aaaaaataaa attaaaataa atattgcaat atttttgaga aggttttata atgagtatat
     1981 aatttgaaac gtgtaactat taaattaggc atgagttctt ggatattttt atcctgagga
     2041 ttcatgcctt ttttgtattg aaaatcaaaa atctatttaa taaggaggaa gtactgtgtt
     2101 aaattttttc aagaaaaata aaagctataa gttacatgca gtagtgagtg gtaactgtat
     2161 taatattgaa aaagtaaacg attctgtgtt ttcaaaaagg ttaatgggag atggagttgc
     2221 aattattcct aatagtaatg ttgttgtagc tccttgtaat ggtaaagtaa ctgtgcttac
     2281 agagtcaaag catgcatttg gtatggtctc agatgagggt gttgaaatat tagtccacat
     2341 aggaattgat acagttaatt tacaaggtga tgggtttaaa aatgaagtgt ctcaaggaga
     2401 taatgttaaa aaaggtagtc caataataag ctttgaaaga gaaaaaataa attctcaggg
     2461 aattgattgt acaataataa tggttgtatt aaatcatagc gaattttcag aaataaattg
     2521 catggtagaa aatgaagttg ttgcagggca agatactgtt attgaaatta tgaaatgata
     2581 ggaagggatt aattcaatat gagagtagta aaaatcttta ataataatgc attatctaca
     2641 gtaactaatg ataggaaaga agctatttta ctaggtcttg gtataggttt taataaacgt
     2701 ccaggagata aagctaatga agataaaata gaaaagattt attatgttca agaccacatg
     2761 caaacaaaat ttttagaatt gttaaaaaat gttagtccag aagtgatgga tgcttcacaa
     2821 caaattataa gtcttatacc agggaatgag ggaaaattta ataataaagg gatattatct
     2881 ttagtggaac atattagttt tgcaatagat agaatgaaaa ataatgtttt tctacctaat
     2941 ttaatgcttt tggatataaa gatgatgtat tcaaaagagt ttgagttagg agtaaaagct
     3001 ttagaagtta tatacagagt atgtcatatc catttgcctg aagatgaagc aggttacata
     3061 gctttacatt ttgtcaattt acaatcaaat gataatttgg catatgatac tttaaaattt
     3121 gtaaaaggta gtattaattt gattaaagaa tgttatggat tagaattaga cgaatcgagt
     3181 ttgagtacat tacgttttag gactcatctt aaatttcttg ctcaaagaat ttttaagaat
     3241 gaaatttgtc aggatgataa aatgattgaa atgtatgatt atttaattaa caatcatcct
     3301 aaaaataaag aatatttaga aaagttaaat atatatattg aaaaggagtt taggtataaa
     3361 ttagataaac ctgaaaaaat ttatctactg ctacacttaa caaaaatctt ataagaatga
     3421 gatgaattag taatagaaat atatataatt ttaagggggg ataagattga aagataaact
     3481 acttggagga ctggaaacat ttgcaaaggc aatggttcag ccattaatgt atttatcaag
     3541 tgcagggata ttaatggtgg taggtgtact acttacaaat gatatgttac taggagtatt
     3601 tccatttttt aaatgggagc caattcaaat atttggaagc ctaatttata actgtataat
     3661 ggctattatt aataatcttg ctgttatatt tgctataggt attcctgctg cactagcaaa
     3721 aaaagagaaa cacaaagcag cattaatagg atttatgagt tatataatat accttacagc
     3781 ttctaacact atgttaacat cactaggtca aatagcaaaa gaaagcaaga tgcttgggtt
     3841 gatagggact ggacaagcta ctattttagg tattcaagtt gtagatacaa gtgtatttgg
     3901 tggaattatt ttagggtgta tagctggata tgtatttaat cgtacaagta acaaaacatt
     3961 taaaggagct ttacagatat actcaggagc aaattttagt tttatatgta tgttctttgt
     4021 atccatttta attggcgttg tgagtaactt tatatggcca ttagctcaac aaggaatagc
     4081 ttctcttgca aatgtcatca aagatacagg tacatttggt ttattcttat atggtttttt
     4141 agagagaata ttagtaccaa ctggacttca tcatttaatc tacacaccat ttcagttttc
     4201 tgatttagga ggagtgctta aagttggtgt agatacagtt tcaggagctt atgctatagt
     4261 tatgacagaa atgaatttac cagttgagcg tttctcagat agtatatact acatggcaac
     4321 tggatttaca aagatgtttg gttatatagg tataggagct gcctttattt atactgcata
     4381 taagcaaaat aagaagaagg ttaaggcaac gattattcca ctaataatta cggcagtatt
     4441 agcctctgta acagaaccat tagactttat gtttgtattc tgtgcaccac ttttattctt
     4501 tgtacatgca gttatagctg gtctatttat agctttatta aaaatatttg atgttactgc
     4561 cttatgtgga gggaacttat tagtatcaat tataatgaat actacagtag gtgtagaaaa
     4621 aacacactgg ccagtaatga tgatattagg acttgcacaa attattgtat attttgtagt
     4681 atttagtttt ttaattaaga aatttaatta taaaactcca ggtcgtgaag atgagagtac
     4741 agctacaggc gaaggacaat caaaacaact taatttagat acaggtatag aaaatataat
     4801 tgatgggtta ggtggaaagg aaaatattaa tacagtagaa aattgcatca ctagattacg
     4861 tgttaatgta aaagatgaaa gtaagattaa tgaagatata attaatttaa cgccaaatag
     4921 cgggattgta aggaaaggaa aagatataca aataattttt ggacttcatg tgcatgaggt
     4981 aagacgtgca gtagaagatt ttttagaaat ttattaaaga tacatataat ttatatagag
     5041 tttatagagg aggaataatt attatggtta ttactattgt tggaggagga agcacattta
     5101 ctccaggtat tgttaagtca attgcattaa gagaaaaaga gttagagctt gaagaaattc
     5161 gtttatttga tattgataaa gaacgtcaag ataaagtagc tgtagttgtc aactggatac
     5221 taaaagaaga tgttaaaaca gatgttaaac taactgtaac taattgtgaa aaagaagctt
     5281 atacagatgc tacatttata tttgcacaaa tgcgtgttgg taaatatgaa atgcgtgagc
     5341 aagatgaaaa aattccattg aaatatggtt gtgtaggtca agaaacttgt ggttgtggag
     5401 ggatggcata cggattgcgt acaatacaac caatgataaa ggttattgat gatgtagaga
     5461 aatatgctaa aaaaggttat tggatattaa attactctaa tcctgctgca atcgtttctg
     5521 aagcttgtcg taagttaaga ccaaatgcaa ggattataaa tatctgtgat atgccaatag
     5581 ctattataga tatgatagcg gggtcattaa atatcaatga cgtccataat atacgctatg
     5641 attattttgg tctaaatcat tttggctggt ttacttcaat agattataaa catagagact
     5701 taatggaaga gattaaggaa tacataaaag aaaataaaat attactacca aaatcttata
     5761 tagaaaactt aaataaaagt cgtagtacag gaaatcgtca tgctgtaagt agctggatac
     5821 atgtttggga atctgtttat acaatgttag aaaacttccc aaaatatcta ccaaacactt
     5881 atttaacata ctatctttta agtaaagaaa cagtggaaca ttcaaacata aatcgtactc
     5941 gtgcaaatga ggttatggaa agccgtgaaa aagacttatt tgaaggtgtg agacattact
     6001 tagaaactgg agaaatttct gaaaaagcat tttatgcagg ctcacatgga gattggatta
     6061 gtgatttggc agtttcaatt aaaaatgata cacgtagtcg ttttttagtt ataactgaaa
     6121 atcgtggagc tatcccaaac atgccatatg atgcaatggt agaaattcca gcatatattg
     6181 gtaaagatgg tccagaagtt attgctcgag gtccaatacc tctattccaa caaggattaa
     6241 tgatgcaaca acttaacagt gaaaaattga tagtagaagc ttatatagaa aatagttatg
     6301 aaaaagcttt acaagcattc acattaaata aaactattcc aagtatgaat gttgctaaag
     6361 aaattttgga tgaaatgata gaagttaata aggaatactg gccagagcta agatagtagg
     6421 atagaagaaa aggtttttat ttaataatta ggtatgtttt cagagtcttt tatcaaaatg
     6481 tattcacaaa catatggcaa tatattaaag taaagagtga tatttttaga agaatggggt
     6541 gatgcaatga acattataaa taaaattttt tggcaagaag ataggattgg catgattaca
     6601 aataatttag aggcagatac acatagtcac tgcatgttac aaatgttctt agggatagaa
     6661 gaaaacattg aaataatagt caatgaaaaa ttagttaaat gtaattgtat cgtagtagat
     6721 aaaaatatct ctcactcatt ttcagcaaga aaaaaagttt attactctgc tattattgaa
     6781 cctacatcta tatatgcaga acagcttacc tcaaaaatga gtgattttgg ctactggata
     6841 tgtgacaatg atggattaga gaaattacgt caacaaggaa cttttttgat atataattct
     6901 agtaaagaac aatatttaag atttatggaa atgcttaata actatttgaa tatacctata
     6961 actctcaaaa attatgatga cagaattact gaattgctca atttattacg tacttgcaat
     7021 tgtgataatc atactatttc aagttttgca gataaagtgt ccctatcagc aagtaggcta
     7081 tcacatctgt ttaaataaca aatagggata cctttaaaaa gctatatctt atttcatcaa
     7141 atggagtgtg cttttagaga attactttct ggaaaaaatg ttaccgaaac ttctatgact
     7201 gctggattcg atactccatc acactttgca gggacagtta agcgaatgat gggaatgccc
     7261 gtttctttat ctttgaaaga tagcgagttt ttgaaagtat attgaatatt ctcgttatat
     7321 aatgaagttg tattaagaaa ggggctttat tatgaataaa gaatatttag aagaaacaaa
     7381 aatgttaaat tataatgaac cacaacttaa gctcttggta tcatcaaaga agtggttgga
     7441 attagatgat ttttataaaa taaaatctat ttatgagttt gtacagaatg atattttatt
     7501 tggatataat gcttctgata tattaagcgc aacacaggta ttaaatgatg gtatgggaca
     7561 atgcaataca aaagctactc ttttaatggc attattacgt gcagtgaata tcctttgtag
     7621 gcttcatgct tttgatgtaa caaaagattt tcaacgaggt gctacatcca aattgatttc
     7681 actattagca ccaaaatata ttcttcatac ttgggtagag gtattttatc aagataggtg
     7741 gattgctctg gagggtgtca taactgataa aaaatattta gaagcaattc agaagaaatt
     7801 ttctaattat ggtggaacgt ttaaaaagta tgcaatagct acaaatgatt tgaaaaatac
     7861 atctattgat tgggatggta aagatacttt tatccaaaaa gaagctatcg tgtatgatta
     7921 tggtattttt ccatcaccag atgtattttt ttctactcat tcacaacata tgtcaaaatt
     7981 aaagaatttc atatatgtac atcttatcag aaaaataatg acaaaaaatg tatgtaaggc
     8041 tagaaataat tatattgata aaaacgaatg agtgtttaag aagtgactat ataggaaata
     8101 tatagaacaa ttaaaactgt attggttaaa atgaataaaa aggagttatc aaagagcaaa
     8161 cttaaattgt tcataggata actccttttt tatattaact tctaataatt tggtgtcttt
     8221 ttataaaaca tatagataca attaaaccaa caaatgcaag agtagctgct attgaaaatg
     8281 caacatctat tccatggata ttagacagta tagcagaact catgtgagct tttttagtag
     8341 cacttgtcat tatagtaata agcacagcag aacctatagc accagatatc tgacgaagtg
     8401 tactattaat cgctgtggca tgagatatga gttctctgtc tagtgtttta attccccatg
     8461 tagttaatgg catcaaaacc attgagatac caatcatacg aatacaatac atcaatgata
     8521 catgaagtaa agatgtatcc ctacctaaaa aagcaaaaga tagagttcct aaaaataaac
     8581 atccagttcc taatatcgat agagcatgag gaccatattt atctaaatga cgtcctgcaa
     8641 ctggatttaa tacaaccatt aatatagcgc caggtaacat taatgaaccc gaagacaatg
     8701 cagaaaatcc tcttacagac tgaacatata gaggaatcat taatgtagca gacatcattg
     8761 aagcatatac aatcataatt aatattgtag aaatgagaaa tggtttattt ttaaaaaccc
     8821 tcaattctaa aaatggttct ctagatttta attgacgtag tgtaaagaaa attaatgata
     8881 taattcctat gaatattggt aaatatgtgg caatattagt ccacccataa ttaccttgat
     8941 tagtaaatcc aattaacaaa ccaccaaaac ctagagatga cagtattatt gaaggaatat
     9001 ctaattttag tttttgcgtt tctccaacat tttttagtag aataaatgaa agtataacat
     9061 ctagtatagc tataggagaa agaagcaaaa ataaactatg ccagccaaat gaatctacta
     9121 accatcctga taaagttggt cctattgctg gagcaaatcc tactgtaata ccaactaaac
     9181 tcatagctgc accacgtttt tcctcagggc ataaatatag tataataact tgtaaaagtg
     9241 gcattagaga accagaaccc attgcctgca acacacgagc aaccaacata gttgaaaaat
     9301 tattagaaaa aagtgcaata atacatccta tagaaaatat agacattgat gtaatgaata
     9361 actttctagt actaaatcta tttatcaaat aggctgttgt gggcacaata atacctgcaa
     9421 taagtagata tattgtcgtt aaccactgac caacatcagc agtaatttta aaatctgaca
     9481 ttatattagg taatgctgta gataatagtg tttgactaag agaacttata aaagcaccag
     9541 tcattacgat tgctattatt aaatttcctt ttttattgtt actttgggtt tccataattt
     9601 acaacgtctc cttttttaat tgatttttaa tgctgttcaa taaaagtacc tttggtctca
     9661 aagtgtcttc tttttccaaa tatgcaatca ttgaatccat aattaaagaa tcatattgtt
     9721 gattttttcc ctcgacatat aatttttttg ttgcttctag taatttagaa ttttcaagga
     9781 gagaagtatc tgttttgatt agttcttcta tatcttgtat cgttttaaaa aatagcgatt
     9841 tattataaag ggaaaagtaa gcttcatctt cttgtttatg taaaaacatt gatttttcaa
     9901 ttaaaggatt tagattatcc atattttcta caactaaatc tagaatttgt atagtaaatt
     9961 tagcaacaaa gtatacattt atttctcttt tctgtacatg aataatgtat ataaactgat
    10021 gaattagagc atccaataag aaaactaaat caaatatgat gttttctatt ttatcaccat
    10081 agttatcaaa taagcttagc ttgtattcat tcaataagtt agctcttgat tttgtttgca
    10141 atttacttgt agtcttacgt tgcacttcag aaaaattttc tcttatgtag gtatttatgg
    10201 tagttctaga aaaaatattt tcccatacag caattatctt tctgtttaac ttttcagatg
    10261 atggaatatc attattttca ttgatagaat gtataatttc taaaaacttt tcatcataat
    10321 aattgattat ttcgcagata aaactttcct tagtttcaaa atgcttgtaa aaagtagctt
    10381 tagacatttt gcactctttt acaatgtccg agattgaagt attttgaaaa cctttttggt
    10441 caaaaatttt ttgtgcaata gttataatta gcctttgttt ttctgtcact ttattacctt
    10501 ctttcatata aaaaacgttt agtactatat agtacctata agttttaata aatatattat
    10561 acatcatttg gaaaaaaaat caatatatac actgtaatat gaaatttttg aaaaatttat
    10621 tgttattttt agtcgtataa aaaagaaact taagagtttc ttaagaattg tttaaataaa
    10681 ttgcaataaa ttagtggtat gattatagta tgaaagagaa tgtcttgaaa tttttctgta
    10741 tcaaattcca attatggagg tgtagtatat gaaaaagaaa atttgtgtag ccataatttt
    10801 tgctctattt atagtttttg tagaaatgtt tattgatagt tctagtgaag gaagtaaaat
    10861 tatgacagct gaattaagta aatacaggaa gggcgtaaat gtattatcaa taaacaatat
    10921 tatagtagcg aataaaaaat atagtttacc taaagactat tctcctcagg aaagtagtga
    10981 agcgagaaat gctttttata aaatgaataa agatgctcaa aaatcaggat taaatttaaa
    11041 agcttttagt acatatagaa gttatgaata ccaagataga ttatttaagt catatgtaaa
    11101 agaacatgga gaaaaagaag caaatagatt ttcagctaaa cctggagaaa gtgagcatca
    11161 aactggactt gcttttgata ttggggggaa tgaccagtct tgttgggcaa ataaaaagtt
    11221 taacaataca aaagaagcta aatggttgta tgaaaatgca tataaatatg gttttatact
    11281 tagatatcca gaaggcaaag agcatataac aggatatatg tatgaatctt ggcactatag
    11341 atatgttgga actgaacata gcaaaaactt tgcaatgaat aacttgactc tagaagaata
    11401 tttacatata aattaagaga tagtttgtta aaattaggtt aaaataaaaa ataaactaga
    11461 ctattaattg tgtaaagtga tataatgtaa atataattta agataactac tcgaagggga
    11521 gtagcttaca cgaaagtggt aattaatcgt caagacagta gaaatactcg gttaattagc
    11581 aagggattcc ttgatagcaa gaccttcaaa ataagcatta ggtatgtttg ttttggggtc
    11641 tttttttatt caaggaattt ttatttaaaa aaatttaaat ttcaagtata cataagagcc
    11701 cccaaacata aaataaattt attgaagagg agggcaacaa atgtcttaca aaagtactta
    11761 tcaagaagta attaaaaatt tagactcaag tttatctggt ttgagtaaag aaagagcaga
    11821 agagttgtta gaaaaaaatg gagctaatga attaaaagaa gcagataagg ttcctactta
    11881 taagcttttc ctagaaagtt ttaaagaccc attagtaata attctattga tagctgcttt
    11941 agtacaaata tttttaggtg aaacagtgga gtctataata atttttgcag ttattattat
    12001 aaactctgtt ctgggtgttg tacaaacaaa aaaagcagaa agttcattag aaagtttgaa
    12061 aaatttatct gcaccaaacg caaaggtaat aagagacaat aaaaaaatga caattccagc
    12121 aaaaaatcta gtagttggag atattgtatt cttagaagct ggcgattaca taccagcaga
    12181 tggaagactt atagaagcac aaactctcaa ggttgtagaa ggtatgctaa ctggagaatc
    12241 agaaccagtt ttaaagcata cagaaaaaat aagtgaagat gtaagtttag gtgaccagaa
    12301 aaatatggta tttagtggct ctgtagttgt ttatggtaga ggttctttta tagtgaccgg
    12361 aacaggtatg gatacagaaa tgggtaaaat agctgggctt ttagaaagtg ctgaaaataa
    12421 acttacacca ttacaacaaa aaatagagga ttttagtaaa aaacttggtg taggcattgt
    12481 aatacttgct cttttaatat ttataattca agtagcaaga aattacttta tggttggaaa
    12541 tggagaaatg acacaaatta ttttagattc attcatgttc tctgtagcag tagcagtggc
    12601 agctatacca gaagctttat cgtcaatagt cactatagta ttggctgttg gaacaaatag
    12661 tatggcaaaa aaacaagcca taataagaaa attaccagca gtagaaactt taggttcaac
    12721 aagtgtaata tgtactgata aaacaggtac tcttacacaa aataaaatga cagtagttga
    12781 ttattatatg tatggaacta ataatgatga gttagataaa gaaaaagtaa actattctta
    12841 tcatgctaaa ttattgacta tggtttctac attgtgtaat gactctcata taactagtga
    12901 tggtaaagaa gttggcgacc caacagaagt agctttaata aattactcaa gtaaaaatga
    12961 tttagattat gataaattaa gaagtaagta tattagaata aatgaaatac catttgactc
    13021 agatagaaag cttatgtcta ctgtaaatag tatatatgga gactatataa tgtttacaaa
    13081 aggtgctcca gatattgtgt ttagtagatg taaatatgct ttgaaaaatg gttcaaaagt
    13141 agatataaca gatgagataa tagaagaata taaacataaa aatgaagagt tttcaaatag
    13201 agcacttaga gtattagctt ttgcaataaa agatgttcca gaagatgatt ttgtaccttc
    13261 aattgatgat gaacatgaca tgacattggt tggaataatg gcaatgatag acccaccaag
    13321 agaagaagta atggatgcag taaaagaggc aaagagtgct ggtataaaaa ctgttatgat
    13381 aactggtgac cataaaacaa cagcagcagc aattgcaaaa gagattggta taatggaaga
    13441 tggagaccta gctcttactg gaaaagagct tgatgctttg agtgaagaag agctatatga
    13501 aaaactagaa aatataagtg tatatgccag agtctcacca gaaaataaaa ttcgtatagt
    13561 aaaagcatgg caacataaaa ataatataac agcaatgact ggtgatggtg ttaatgatgc
    13621 acctgccttg aaacaagctg atataggaat cggtatggga agtggtactg atgtagcaaa
    13681 agatgcttct gccatggtat tggttgatga taactttgca agtatagtaa atgctgttga
    13741 agttggtaga acagtataca gtaatataat aaaatcaatt acttatctat ttgcaggaaa
    13801 cttaggagct atagtatcca tattatttgc agtatttgct ggttggtcaa atccatttac
    13861 agctttacaa cttttattta taaacttagt aaatgactct ttaccagcaa ttgcattagg
    13921 gtttgaaaag tcagaaaaaa atataatgaa taaacctcct agacatccgg atgaaagtat
    13981 actagcagga ggaacattga gggcagttgc tataagaggt tgtataatag gtatagttac
    14041 aataattgca caatatatag gattgcaagt atctccagaa cttggagtag caatggcatt
    14101 ttcaacattg atattagcta gaataatgca aacattacct gcacgttcta ataatcaaac
    14161 agtaataaag ttaggttttt ttagtaataa atacgttata ggagcagttc tagtctgtct
    14221 tgggctttat tctttaacac taataccagt gctaagagga atattctcta taccaacaac
    14281 atttggattg tatcagttgg gaatttgctt aggtttagca ttgatttcat caatattaat
    14341 ggaacttaac aaattaataa acagaagtga agtaaaataa ataaaaaatt aaagggcata
    14401 ttgattttaa caatatgcct tttataaata gtctaactta ttctatatta tcaattactt
    14461 ggaatgcctt tataacttca ggtaattctt tttcttcagg cattctaaat cttatgaaat
    14521 ttttatcaag tccagtaaaa tcacttccag atataactct tatttttaat ttagcaaact
    14581 cttgttgcaa atctatgtcc ttgttcttat gttcaactgt cataattgat acagtatcac
    14641 tagtttcagc tatattaagg tttttccatg gaattaataa ttgatttttt atatcttttg
    14701 ttttttcttt taattcttct ataaattgga catcatctaa taaattggct gcaatactac
    14761 gagatatttc agatacttcg taaggagtag aaatcttttt aatagggtta acaagttgtt
    14821 ctggaagaac tgcatatcct gctcttagtc ctgctaaacc aaaacctttt gaaaatgttt
    14881 ttaaagcaat tacattatca taattattta aaagcttaac agctgaattt tcttttggca
    14941 tgtattcacc ataagcttca tctaccataa ctgctatatc atatttagct gcttccttta
    15001 ctatattttc aattgatgat aaaggtataa tttgtcctgt aggattattt ggattgtcta
    15061 tataaattac tttatattct ggatttattt tttcaataaa ttctttttca ttaaacttga
    15121 aattatcctc tttctttaaa agaacatagt cataagtagc accatgcatt tttatatctg
    15181 tttcatattc tgaaaactga ggtgaatatc ctaaaacttt atctcccttt tcaataaata
    15241 atctatttaa aaggtaaata acatgtatag aaccatctgc caaacaaatt ctgtcagtgt
    15301 ctaatgttaa gaagtctttc caataatcga ctatattgtc ttttaaagca attgaatgag
    15361 gatatcctct tatcatatca aattttaaag cattaaaagc ttctacagct ttgtttgagt
    15421 aagatactgt atttatacct tcaccacagt ctatctcgaa tacatcttct gaatgtccag
    15481 attctattgc ataacttttt ttcaactgca tttcaatagc tttgttaatt tttaatttca
    15541 tagtttatca ctccttattt agtcaataat aaatgttata aaagttgcta gatttaaaat
    15601 attatgaaaa tatataatag taaaaaatga atataaatat tataatagct cactttattt
    15661 aatatgatta tatatcatat attgtaaaat ttctagcatt ataagactat aaattgaaaa
    15721 tattcagaaa aatactacaa gaaacgattt attttgctct taaaataagt taaatgaatg
    15781 ttattaattt tgtaaattga aatagtttaa gtataaagtt atgatataat ctaataaggg
    15841 taattcttta gattatatta aggaaatata agtatacaat aaaaagttat ggagttggat
    15901 tatgggatat aaattaattg taacagatat ggatggaact ttattaggaa ataatcataa
    15961 agttacagat gaaaataaaa ctgcactaca aaaagttata aaaaacggta ttaatgtaac
    16021 tttagcaaca ggaagagcct tcgattcagc taaatgcaat gtagattttc ttaaagagga
    16081 catgccaata atagcttgta atggttcatt gataagagag caaaacggaa acataatata
    16141 ttcaaataag attgatacaa caacttgttt aaatatctta gatgtattag ataaatacga
    16201 tatttattat caatgtaata gtatagattc tatgctttca aaaaaaatag aaggacgaga
    16261 agatagatta agtgtatttt taggctctga gacagaagta attataagag atgatttgag
    16321 agaagaaatt tttaaaaaag atatactaaa atttgtaatc atagaagaaa agaatccatc
    16381 tattttggat gaaataagaa aagaattgag aaaagttcaa ggaataaaga taacctcttc
    16441 atggccaaac aatattgaag tcatgaatga aggtgttgat aaaggtaatg cagttaaaat
    16501 tttagctgaa aaaatgaata tagatagaga agatataatt gcatttggag ataattacaa
    16561 tgacatagaa atgataaaat ttgctggtct tggagtagct atgggaaatg cagaagaatt
    16621 gattaaacaa gaagctgatt atgttacaga tacaaatcaa gacagtggag ttgcaaaggc
    16681 aatttataaa ttcctagagt taaaagaatc acttagttct taaaatgata tataattagg
    16741 aaaaaatagg ttgactattt tgtagatagt aattataata caaatagata aaacataaaa
    16801 atttagattc agtgaaaaag agagtagata tggagaaaat ctcagcgaat tggggatggt
    16861 gtgagcctaa tgataacact ttatattgaa aagaactttg gagaatacta cttgaaattc
    16921 agtatggtag taggtaatct cgccttaaga gaatagagtg ggtaaacatt gtttgccaac
    16981 tagagtggta acacggaata tttcgtctct tggtcttttt gaccaagaga ttttttagtt
    17041 tcattaagct ataagaatta taattaaaat acaaaaaaat aaaatatgag gtgaaagtta
    17101 tgaaattatg gggcggacgc tttagaaaag cagaaaatca acttatggaa gaatttaata
    17161 aatcttttgg ctatgattgt atactgtaca aaaaagatat cgaaggaagt gtagcacatg
    17221 ttcatatgca agtaaaatgt ggacttttaa cagaagaaga aggaaagtct ataacagaag
    17281 gattaaaagg gatacttgaa gatgtagaaa atggaaagtt agcattagat ggagagtatg
    17341 aagacataca tagttttact gagataaatt taattcaaag aataggagat gttggaaaga
    17401 agcttcatac agcacgtagt agaaatgacc aagttgcagt tgatatgaga ttgtatgcca
    17461 aagaaaaagc aaatgatttg gtagatttaa tatctgaatt taaagcgact ataaaagatg
    17521 ttgctgataa aaacccggtg atgatgccag gttatactca tcttcaaaga gcacaagtag
    17581 tgacatttaa gcatcactta atggcatatt acagtatgtt tgatagagat gagaaaagaa
    17641 taaaaaatgc tatagaaata ttagatgaat ctcctttagg ttgtggggct ttagcaggaa
    17701 ctacacatga tatagacaga agtataactt gtgaacaatt aggctttaaa aaagtagtag
    17761 ataattttat ggatggagta agtgatagag attatttatt agaactaatg agtgactttt
    17821 caattataat gatgcactta agtagattaa gtgaagagtt aatactttgg agttcacaag
    17881 aatttggatt tgttgagata gatgatttat atacaactgg aagtagtata atgcctcaga
    17941 agaaaaatcc agatggtgca gagcttataa gaggtaagac tggcagagta tatggaaatt
    18001 tatttggtct ttttacagta atgaaaggaa taccattagc atataataaa gacatgcaag
    18061 aagataaaga aggtttcttc gatagtgtac atactcttga aatgtgtata caaataatgg
    18121 atagaatgat agctacacta aaggtaaatg aggataaaat gaaacaagca gtaaagaatg
    18181 gttttttaaa tgcaaccgaa gttgcagatt atttagttaa aaataatgtt gcttttagag
    18241 atgcacatgg tatagtagga agtatcgtta tatattgtga agataataaa aaagcaatag
    18301 aagatttaac attagaagaa ttatataagt tttcagatgc ttttaaggaa gatatttatg
    18361 attttataga ctatgagagt atattaaata agggaataaa aaagaattta aaataatata
    18421 cttatttcta aaagctgtag att
//
LOCUS       DAFNNB010000070        18393 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0071, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000070 DAFNNB010000000
VERSION     DAFNNB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0071"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            264..1424
                     /locus_tag="L3M60_002033"
     CDS             264..1424
                     /locus_tag="L3M60_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF819 family protein"
                     /protein_id="HBN6226697.1"
                     /translation="MIETGFVYCSFLVVFVGVIFFMVDKLKWKVFDVINPMLLIYLGS
                     AILGSFGLFAQNEDVGIYQNIITVNFLPLMLVFLLIQCDLRKILKMGPKMLLSFFCAT
                     FTFMLGFVIAFIIFKNSMGTGSWKILGAASGSWIGGSSNMIAAANALGVAEEGLSYAI
                     LMGSIGYTVWMSVCLMSVKFGPRFNKWTKCDTSFIDEVTSKLEKENKDNTAPTFVELM
                     ILLSVGFGVASFAQIVSKILPTSGILNETMWVVLVASTVSILLGMTKISRLKGSTLVG
                     NVFMYLLLAATGSKANFFGLSEAPVYIMFGLVVVLVHAISFIGLAKLFKLDLFTCEVG
                     SIANIGGVTTAPMIAGVHNPVLIPVGVLMGLLGNIIGTYCALMVTQILHMLS"
     gene            1764..1925
                     /locus_tag="L3M60_002034"
     CDS             1764..1925
                     /locus_tag="L3M60_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226698.1"
                     /translation="MIIPSRRIQDITLTNLKNGEVTLIELDEIYKKMGFLFVVSEGKL
                     KKIKKENHH"
     gene            2118..2747
                     /locus_tag="L3M60_002035"
     CDS             2118..2747
                     /locus_tag="L3M60_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBN6226699.1"
                     /translation="MKIVTYNIHKGMDSNNRLTLTKMGLYLKQLNCDVICLQEVLYPQ
                     FLALKAVLNMDGVFATNVKKVNMIYGICTFTTFKMLNSNHFFLTSKKEQRGALCITID
                     AYGRIINVINTHLGLDRQERAKQLDEIIDYRNRLVGIVVLCGDFNEKNVFLSMFNDMA
                     ISLNKSYLSTFEKSNSRIDYIFVNKNTELKGYTVEKIYLSDHYPVIGYI"
     gene            2987..4246
                     /locus_tag="L3M60_002036"
     CDS             2987..4246
                     /locus_tag="L3M60_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HBN6226700.1"
                     /translation="MGRRATRVSRLGKEKKRNIVIVLVVLIIAMGGFTYFFNSKFLYN
                     GKIAKNVYIEGVNVSDMTKSEALRAITDKYTPEDLNLTYDGKKYTISPKDIDLKYDTE
                     DVVKDAYESTKKGSYFQNLKKYIDIRVNKANMKIKAEYDEAKLSSKVSSIADSINVKM
                     KNASISVGSGGLNYTDSVIGREFDLAANKESIYNMIKNKEHKTLELKVNLQKPDITTE
                     QVKTVNSVIGQYSTTYSQAVEGRSYNVGLSARKTSDVLLMPGEEFSYNKLTGPSNKAN
                     GYKDAPVIVYGKLEQSAGGGVCQTSSTVYNAALLAGMEITQVTNHSSASTYVPKGRDA
                     TVSDGGLNLKFKNPYKHPVYIKNYAGGGSVSSVIYGNSGDKPNISIEVKQTGQNKYST
                     YRIFKDSSGKVIKKEHISNSSYKELKK"
     gene            4415..5410
                     /locus_tag="L3M60_002037"
     CDS             4415..5410
                     /locus_tag="L3M60_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HBN6226701.1"
                     /translation="MYEEVTWLNFIGNRYEVVNSDDVLEINKIYKARDVFYRKNVLIK
                     VIKHNNYICEDFVSNLIDESTALDEINSPYILKIIDVGIHCTEETTLYYVVSEDFNGI
                     GLDELILGNYLHLEAIVNIMIQVLKALEMIHRNHSYHGSLKSSSIIVDTEYNIKICDF
                     GITKANNGVNTRSYGNRRYLCPHQLCINYTDKESDFFATGLILFESIFKKLPFGESNL
                     EEKMLQSIDRGLDWNSIKAINGNTELINVIKRLLGRNNKYTRANDIIIDLSKVMYEKA
                     YIEVEDEKEEEQKQDKVVQVKKDYKSKMRKKLHKKLVVAGLAIVMISMIIISSII"
     gene            5656..6723
                     /locus_tag="L3M60_002038"
     CDS             5656..6723
                     /locus_tag="L3M60_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBN6226702.1"
                     /translation="MSNIKIIASNKCWIEDIAKKQLEDISKLNGILRVVGLPDLHAGK
                     IPVGLAVETKNIIYPHIIGNDIGCGMTLFKTGILKKKFKKDKWIKSLSKIKDLSDIEI
                     KNTYKEECPILNLGTIGSGNHFVEIQCISEIYNKEQFEQLKFSSDDIMMLVHCGSRNY
                     GEDILKKFYAKDGLEVESEKAIDYIKNHDNALMWAERNREAISKKIMQSIGTSGDVET
                     IFSINHNFIEKREDKFIHRKGAVSSERGAVIIPGSRGSLSYIVMPTENTKISLYSLSH
                     GAGRKWSRSVCKSRLKSKYNKDTIKQTKFKSQIICSDLNLLFQEAPEAYKNIEQIIES
                     LMEHKLIQVVATMKPLITYKG"
     gene            7237..9147
                     /locus_tag="L3M60_002039"
     CDS             7237..9147
                     /locus_tag="L3M60_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HBN6226703.1"
                     /translation="MNSNTFERLQLNEVKELIKIHCVSSLGKNLIDKLNPSGNIGVVR
                     RKLAENKEARKIIENSNHVPLEGLFNAGSTIDKIEKGMVIEPIELVNIEDFLRGCRKM
                     KAFMLEKEFYSPTLSSYALNITECKNIEDEINYCIKSNKVDSNASKELKKVRRNIEIT
                     EAKIKDRLNKFITSTVNKKYIQEFIISKRNDRYVIPIKSSYKNEVDGTILDTSSKGNT
                     VFIEPISVSNLSTELIMLKADETIEEYKILSYLTELIFEKISQIKLNIEILSEYDMVF
                     AKAKYSQKIKGITPKINNNGYIKIIKGKHPLLTGDAVPLDFEIGKNYRSLIITGPNAG
                     GKTVTLKTVGLLTLMVQCGFDISAKEGSEFSVFEKVFVDIGDNQSIENALSTFSSHIK
                     NIAEIMNLSNNSTLVLFDEIGSGTEPNEGAGLAISLLEEFYKMGCITIASTHYGEIKK
                     FATLHPEFENAGMMFDKETLEPLYKLTIGKSEDSNALFISKKMGIKNRVLDRAKEYVI
                     DRNYNLDLVKRSKLQSNVEDKNSSEVILNYTDYNVGDKVKLLDEDDFGIVYKPIDKFN
                     NVEVLFNDNFISVNIKRLQLSIKASELYPEGYDLDTLFTNFKERKLEKDIQRGSKKAL
                     KKIQKEIRENRK"
     gene            complement(9210..9833)
                     /gene="ytaF"
                     /locus_tag="L3M60_002040"
     CDS             complement(9210..9833)
                     /gene="ytaF"
                     /locus_tag="L3M60_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation membrane protein YtaF"
                     /protein_id="HBN6226704.1"
                     /translation="MIESILLVTSLCIDAFVTSFGYGVNKVKVPLYSNIIISVVCSAI
                     LAISLFAGTIVRDFLPQNITMLICFFILFFLGSARLIESFFKSYFKKKSIHSKNYNFN
                     MLDLKLNFNVSIEPDVLDTSKSKVLKSAEAFTLALALSLDGLAIGFGSGLVGVNYIQV
                     ILFSLISNIIAVLFGCLLGNMFVKNINLNLSWLSGLILLVIGFIKIC"
     gene            10021..10665
                     /locus_tag="L3M60_002041"
     CDS             10021..10665
                     /locus_tag="L3M60_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6226705.1"
                     /translation="MEQHSKVGNFGDLLKILLDEKELSMGELSKKSGIDKSTISRIAN
                     NKQKPNINHLEKMAIHLNINLEELLKASGYEFKNNNNQIFNTDSDFSNFDDILGFANL
                     INDKNFNGNIEKELSKCKLYVQTDEGKKLLFDNFNKKIDSIEKQGQFTDRLRQMYADF
                     CTDGLTIKKYLLIGSMLLYFVISTDVIPDFVFPIGFMDDLVALNIVTKLLKNDK"
     gene            10870..11928
                     /locus_tag="L3M60_002042"
     CDS             10870..11928
                     /locus_tag="L3M60_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HBN6226706.1"
                     /translation="MNCKWISKIDERKKCDREADSSGYCIFHKENKSDEEIQLMVDTL
                     NKEEISEFNGFVFENEFNAEEILTYNYKILDFSESIFKQKANFKKYIFKKNIIFNYTE
                     FKDKVLFNGCVFLENCDFNRTIFSKDYINDRIFEKVKFKGSDLIVNKVENFPRMDGII
                     FSMCTKFVLKNVEYGKSEYEHGKINYRIARNQATKIGEYEMIGFYYYKERIYSSKIMK
                     RSNYPTFSDYLVEKFFDQIARYTTGYGEKPWNILLVIIVIISVFALLYLFVGIESSNS
                     TLVALDINNIGDYSLSEIFRMYMDLWYFSMATFSTVGYGDMVATSLIGKALAGIEVFF
                     GVTIGAIWASVIIKRMIR"
     gene            12032..13216
                     /locus_tag="L3M60_002043"
     CDS             12032..13216
                     /locus_tag="L3M60_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBN6226707.1"
                     /translation="MKNALKKIISLAMILVFIIPNYSYADDKSVDKYSKTSILIDQET
                     GRVLYSKEPDMKVPLASLSKMMTFLLAIEAIENKEVKETDKVKIDKSIASVKGSSYKL
                     KDGEEVPLIELMRGLMIVSGNDAAIAIAKHICKTKEAFVDRMNKKAKEIGMSNTHFLN
                     PNGLPIYDLSNPKKPAKENISTAKDIAILGKYMFDNYEKQVTAITDMETYTNSDRSFE
                     KSNTNALLRIIPEVDGIKTGYTGNAGYCLSFSMVVNKNDKNEKKRRVIGVVLGTNHKN
                     KRTSASLALLKYGKENFNMKKVIDKDSFIGKKYIKGMKELEVTLKAKDELYTILKDDE
                     SIKSEVKIKNIEYPVKKGDTMGIIKYYTSSGDLLGSVDIVSNNSIDNVPLKTKLKMKF
                     AN"
     gene            13423..14136
                     /gene="rpsB"
                     /locus_tag="L3M60_002044"
     CDS             13423..14136
                     /gene="rpsB"
                     /locus_tag="L3M60_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBN6226708.1"
                     /translation="MSVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIYIIDLQ
                     KTVKKVEEAYKFTKEVAETGKPILFVGTKKQAQDAIKDEAERCGMYFVNERWLGGMLT
                     NHKTIKTRINKLRELEKMEEEGVFNVLPKKEVIKLRAEKEKLEKYLGGIKDMPELPGA
                     MFVVDPRKENIAIQEAHRLGIPVVGIVDTNCDPEQLDFAIPGNDDAIRAVKLITGAMA
                     TAVIEGRQGAEEEVAEDQE"
     gene            14215..15126
                     /locus_tag="L3M60_002045"
     CDS             14215..15126
                     /locus_tag="L3M60_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBN6226709.1"
                     /translation="MANITAQMVKELRESTGAGMMDCKKALQEAEGNMEKAVDLLREK
                     GLSKAAKKAGRVAAEGLVAIEMNDDNTVASMVEVNSETDFVAKNEDFKVFVKDAACMA
                     LATDKEDIASLLGETHKEGITLQEVLNNRVAKIGEKLDFRRFAKVVTNGQVAGYIHGG
                     GKIGVLVEMETEARDAKVLELGKDVAMQVAAMNPKYVSRDEVDAEYIAHETEVLTQQA
                     LNEGKPANIVEKMVKGRLEKELKEVCLLEQTFVKNPDITVKQLVADVAKAVGSDIKVV
                     KVVRFEVGEGIQKREENFAEEVAKQLK"
     gene            15210..15914
                     /locus_tag="L3M60_002046"
     CDS             15210..15914
                     /locus_tag="L3M60_002046"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBN6226710.1"
                     /translation="MDKPMYKRVLLKLSGEALAGEKGFGINNDVVNDIAIAIKKIQEI
                     GVEVAVVVGGGNFWRGRTSEGMDRTTADYIGMLATVMNAMALQDALENIDVATRVQTA
                     IDMRQIAEPYIRRRAVRHLEKERVVIFGAGTGNPYFTTDTTAALRAAEMEAEVILLAK
                     NVDAVYDKDPKVHADAKKFTELSYMEVIQKELKVMDSTATSLCMDNKIPIKVFELTTE
                     NIIRAVKGENIGTTVK"
     gene            15940..16497
                     /gene="frr"
                     /locus_tag="L3M60_002047"
     CDS             15940..16497
                     /gene="frr"
                     /locus_tag="L3M60_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBN6226711.1"
                     /translation="MKLEIHKQLEEKMNGTIDALKFEFGTIRAGRANAQMLDKIRVDY
                     YGTPTPINQIGAISVPEPRILMISPWDKSAMHEIEKAIANSDLGLNPSNDGEVIRLSV
                     PALTEERRKELAKKASKAAEDFKVRIRNERRDANEKIKKMEKGGELTEDELKKAQDEV
                     QKMTDKFIKEIDTLLSKKEKDIMEV"
     gene            16500..16703
                     /locus_tag="L3M60_002048"
     CDS             16500..16703
                     /locus_tag="L3M60_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226712.1"
                     /translation="MKKYYNLLGLHVDEVKQYFDEQDINYTIKSIEGKKDKDKLVVPR
                     VIKISEIGDSVELIITYFSDSLV"
     gene            16732..17466
                     /locus_tag="L3M60_002049"
     CDS             16732..17466
                     /locus_tag="L3M60_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HBN6226713.1"
                     /translation="MNNNIIYDIDLNNIPTHIAIIMDGNGRWAKARFLPRTAGHKAGV
                     ETIRDIVKECSKLGVKHLTLYAFSTENWKRPKLEVDTLMNLLSTYLRNEIAELHQNNV
                     KVTAIGDISALPKTCIRELNSAKELTKNNTGVNLNLALNYGSRADIKNALIDIVKNCE
                     SGKIDINNIDEDIIKNYLSTKSIPDPDLVIRTSGEQRLSNFLLWEVAYSEFYFTDIHW
                     PDFKKEELQKAIYVYQRRDRRFGGLK"
     gene            17479..18258
                     /locus_tag="L3M60_002050"
     CDS             17479..18258
                     /locus_tag="L3M60_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBN6226714.1"
                     /translation="MLTRIIASLALVPLFLFVVYGGIPLYIAETAIVYIALHEFYKAF
                     KIKDVNPIFIIGYLFSIYLAVKNIFNLPLEYTYAVIFILFLASIIYMLMGKNNVIDVS
                     ITFLGVFYIGVFLDFIIITINEFEKGSIYVWLIFVISFMTDIFAYFSGYLLGKHKLIP
                     KVSPKKTIEGAIGGIIGSTLCCILFGYLFGIDLLQLAIIGSIGSVIAQLGDLFASSIK
                     RYVGIKDYGKIIPGHGGILDRFDSVILVAPFVYSAIKFFIR"
BASE COUNT         7590 a         1843 c         3183 g         5777 t
ORIGIN      
        1 aaatatttag tttaattatc tttacaaata aaaattttag taataaaata attgttgaaa
       61 aagattttaa attcagatta ttcatttcta aagtaattaa tatgcaaatc tttattaata
      121 tgtgtcaata gttaggtata tatgtattta attagcatga tatagataag agtatttatt
      181 ttaattgtct attatctgaa aaagtcttta cttaaaaatc ataaaaaatc aaattaatta
      241 ggaaaagaaa gggtgtgtat aatgtgattg agactggatt tgtatattgt agtttcttgg
      301 ttgtattcgt aggtgtaatt ttctttatgg ttgataaatt gaagtggaag gtatttgatg
      361 ttattaatcc aatgctttta atatatttag gttcagctat acttggatca tttggtttat
      421 ttgcacagaa tgaagatgtt ggaatatatc aaaatataat aacagtgaac ttccttccat
      481 taatgttagt gttcttgtta attcaatgtg atttaagaaa aatactaaag atgggaccta
      541 aaatgttatt atcatttttc tgtgctacat ttacatttat gcttgggttt gttatagcat
      601 tcataatttt taaaaattca atgggtacag gttcatggaa aattcttggg gcagcatctg
      661 gttcatggat aggtggctca tcaaatatga ttgccgcagc taatgctcta ggagttgcag
      721 aagaaggtct tagttatgca atacttatgg gttctatagg ctatacagta tggatgtctg
      781 tttgtctaat gtctgtgaaa tttggaccta gatttaacaa atggacaaag tgcgatacaa
      841 gttttataga tgaggttaca tcaaaacttg aaaaagaaaa taaagataat acagcaccta
      901 cgtttgtaga acttatgatt ttactttctg ttgggtttgg tgttgcttct tttgcacaaa
      961 ttgtatctaa gatacttcca acttcaggga tacttaatga aactatgtgg gtagtacttg
     1021 ttgcatctac agtgtcaata ttattgggta tgacaaaaat atcaaggctt aaggggagca
     1081 cattggtagg aaatgtgttt atgtatttac tccttgcagc cacaggtagt aaggcaaatt
     1141 tctttggatt gtcagaagca cctgtttaca taatgtttgg attggtagta gtccttgtac
     1201 atgctatttc atttataggg cttgctaaac tatttaaact tgatttattt acttgtgagg
     1261 taggaagtat agctaatatc ggaggagtaa ctacagctcc tatgattgct ggggttcaca
     1321 atcctgtatt aataccagta ggtgtactta tggggcttct aggaaatatt ataggtactt
     1381 attgtgctct tatggtaact caaatactac atatgttatc ttaataaaaa tataatataa
     1441 aattaatcat ttaaataaga aaaaacattt caatttaagc ataagctttt attgaaatgt
     1501 tttttatttg acaagataat ttatttcaag aagatatttt gacatattta gcgtataata
     1561 ttgtaaaatt tactaaaaat gagatatatt taatttttat attgaatcga acgtctgtat
     1621 gatgtataat aaatatatag aacgaatgtt tgcagaacgg atgttttttg aatttatttt
     1681 aataatttat gaatatatat cagaatgcat aaatatattt aaatataaag attaaaaaga
     1741 atttaaagga gtgttgataa cttatgataa ttccatcaag aagaatacaa gatataacat
     1801 taacaaattt aaaaaatggt gaagtaactt taattgaatt ggatgaaata tacaagaaga
     1861 tgggattttt atttgttgtt agtgaaggca aattaaaaaa gataaaaaag gagaatcatc
     1921 attagaaatg aatgaaataa taaatattat aatagtttat aaaacctaac ttatgtagaa
     1981 tataaatact taagttaggt ttttttgtga taaagtttat aagtactcaa ttttataaga
     2041 taggtaatag tcttaagtga aaagtaaata agtttaaatt ttaataagta ttatattaca
     2101 taagaggtga tattattatg aaaatagtaa cctacaatat tcataaaggg atggattcaa
     2161 ataatagatt aactctaact aaaatggggt tatatttaaa acaattgaat tgtgatgtaa
     2221 tttgtttaca agaagtattg tatcctcagt ttttagcttt aaaagctgtt ttaaatatgg
     2281 atggtgtctt tgccacaaat gtaaaaaaag taaatatgat atatggtatt tgtacattta
     2341 caacatttaa aatgttaaac agtaatcatt ttttcttaac tagcaaaaaa gaacaacgag
     2401 gagctctttg tataactata gatgcatatg gaagaattat aaatgtaata aatactcact
     2461 tgggacttga tagacaggag agagcaaaac aattagacga aataatagac tataggaata
     2521 gattagtggg gatagttgtc ttatgtggtg attttaatga gaaaaatgtc tttttaagta
     2581 tgttcaatga tatggctatt tcactaaaca agtcttactt gtcaaccttt gaaaaatcaa
     2641 actctagaat agattacatt tttgttaata aaaatactga actaaaagga tatacagtag
     2701 aaaaaattta cctgtcagac cattatcctg taataggtta tatttgaatt tcaatataaa
     2761 aaaattaatt attataagat aaacaatttt tttgttaaga aataataaaa aaaatataat
     2821 aaaaaaatat caaaaataaa actaattata agacataaat ttacaataaa gttacatttg
     2881 attaaattcc ttgtttaaca aaagctattc aaatataatc taaggtagtt ttgaaatggg
     2941 aactctatta tgtaaaatta agttagtgtt aaaatgaggt gaatttatgg gcagaagagc
     3001 tacaagagtt tcaagattag gtaaagaaaa aaaacgaaat attgtaattg tattggtagt
     3061 tttaatcata gcaatgggtg gatttactta tttttttaac agtaaatttc tgtacaatgg
     3121 gaaaatcgct aaaaatgtat atatagaagg agtaaatgta tctgatatga ctaaatcaga
     3181 agcattaaga gctataacag ataagtatac tccagaagat ttaaatctta cttatgatgg
     3241 gaaaaaatat acaatatcac caaaagatat tgatttaaaa tatgatacag aagatgttgt
     3301 aaaagatgct tatgaaagta caaaaaaagg gtcctatttt caaaacttaa aaaaatatat
     3361 tgatataaga gtgaataagg caaatatgaa gataaaagct gaatatgatg aggcaaagct
     3421 tagttcgaag gtgtcttcta tagcagatag tataaatgtt aagatgaaaa atgcttctat
     3481 tagcgttgga agtggtggac taaattatac agactctgtt ataggtagag aatttgattt
     3541 ggcagcaaat aaagagtcta tatataatat gataaaaaat aaagagcata aaactttaga
     3601 acttaaagta aatcttcaaa aaccagatat aacaactgag caagtaaaaa ctgtaaattc
     3661 tgttataggt caatattcaa ccacttattc acaagctgta gagggtaggt catataatgt
     3721 aggattatca gcaagaaaaa ctagtgatgt attactaatg ccaggtgaag aattttcata
     3781 caataaactt acaggaccta gtaataaagc aaatggttac aaagatgcac ctgttatagt
     3841 ttatggtaag ctagaacaat cagcaggagg aggtgtttgt caaacatctt caacagttta
     3901 taatgctgct cttctcgcag gtatggagat aactcaagtt acaaatcatt catctgcatc
     3961 cacttatgta cctaaaggta gagatgctac tgttagtgat gggggattaa acttaaagtt
     4021 taaaaatcca tataagcatc ctgtatatat taaaaattat gcaggtggag gaagtgtatc
     4081 atcagtaatc tatggtaatt ctggtgacaa accgaatata tctatagagg taaaacaaac
     4141 tggtcagaat aaatattcta catatcgaat atttaaagat tcaagtggaa aggtcataaa
     4201 aaaagaacat atatcaaata gttcttataa agaattaaaa aaataaataa ttttttagaa
     4261 aaatatttgt aatataaaaa caaggaagtt tataacttcc ttgttttttt gtaaaaatca
     4321 acactattag ttcttgtttt aatattacaa tcattaatat atagaaaata ttcctatata
     4381 ttggtataat tgtaaataga tttagttata taaaatgtat gaggaggtga catggttgaa
     4441 ttttataggc aatagatatg aggtagtaaa ttctgatgac gttttagaaa ttaacaaaat
     4501 ctataaagct agagatgttt tttacagaaa aaatgttcta ataaaagtta taaagcacaa
     4561 taattatatc tgtgaagatt ttgtttcaaa tttaattgat gaaagtactg ctttagatga
     4621 gataaactct ccttatatac taaaaataat agatgttggg attcattgta cagaagaaac
     4681 aacattgtat tatgttgtaa gtgaagactt taatggtatc ggactagatg aattaattct
     4741 aggtaattat cttcatttag aagcaattgt aaacataatg atacaagttt taaaagcttt
     4801 agaaatgata cataggaatc attcatatca tggtagttta aaatcaagta gtataatagt
     4861 agatacagaa tacaatataa aaatttgtga ttttggaatt acaaaagcaa ataatggggt
     4921 taatacaaga agctatggaa acaggagata tttatgcccg catcaactat gtataaatta
     4981 taccgataaa gaaagtgatt tttttgcaac aggtcttatt ctatttgaat ctatatttaa
     5041 aaaattgcca tttggagaat caaatttaga agaaaaaatg ttacaatcta ttgatagggg
     5101 attagactgg aatagtataa aagcaattaa tggaaataca gaacttataa atgttataaa
     5161 aaggcttctt ggaagaaata acaagtatac tagagccaat gatattatta ttgatttaag
     5221 taaagtaatg tatgaaaaag catatataga agttgaagat gagaaagaag aagaacagaa
     5281 acaagataaa gttgtacagg ttaaaaaaga ctataaaagt aaaatgagaa aaaaattaca
     5341 taaaaaactt gttgttgctg gtctagctat agtcatgatt tcaatgatta ttatcagttc
     5401 cataatataa aaattgtata ttaagcattt taaaatcaat atctataaat gatatacata
     5461 gaaattttag tgtagtctta aattatgaaa ggaagtggtt attatgttaa caaaaaataa
     5521 tgaggtatta aatgtttagg agtattttaa caaatagcca cctgctttta cagtttagaa
     5581 taaaaaagct gtggctaaag gtcacagctt tttgctactt tttaaaggca ggagcatatt
     5641 aaaaaggagc aaattatgag taatataaaa ataatagcaa gcaataaatg ttggattgaa
     5701 gatatagcaa agaaacaact tgaagatatt tcaaagttaa atggaatttt aagagtagta
     5761 ggattacctg acttacatgc aggtaaaata cctgtaggtt tagcagttga gactaaaaat
     5821 attatttatc cacatataat tggaaatgat ataggatgtg gaatgacttt atttaaaact
     5881 gggattctaa aaaagaaatt taaaaaagat aaatggatta agtcacttag caagattaaa
     5941 gatttgtctg atatagaaat aaaaaacaca tataaagaag aatgcccaat tttaaattta
     6001 ggaactatag ggagtggaaa tcattttgta gaaattcaat gtataagtga aatttataat
     6061 aaagaacaat ttgaacaatt aaaattttca tctgatgata ttatgatgtt agttcactgt
     6121 gggtcaagaa attatggaga agatatttta aaaaaatttt atgctaaaga cggacttgaa
     6181 gttgagagtg agaaagcgat agattatatt aaaaaccatg ataatgcttt aatgtgggca
     6241 gagcgtaatc gtgaagctat cagtaaaaaa attatgcagt caataggtac gtctggagat
     6301 gttgaaacaa tattctctat aaatcataac tttatagaga aaagggaaga taaatttata
     6361 catagaaaag gtgctgtttc aagtgaaaga ggagctgtga ttataccagg ctcaagaggt
     6421 agtctatcat atatagttat gcctactgaa aatacaaaga tatctttata ttctctatca
     6481 catggagcag gtagaaaatg gtctcgctca gtttgtaaat ctcgccttaa aagtaagtat
     6541 aataaagata ctataaaaca aactaaattt aaaagtcaga ttatatgtag tgatttaaat
     6601 ttattatttc aagaagctcc agaggcatat aagaatatag aacaaattat tgaaagcttg
     6661 atggagcaca aacttattca agtggtggca actatgaagc cattgattac ttataaagga
     6721 taattttgaa agcaaaaaaa ttaaaataat attatagcta gtcaataata aaacctataa
     6781 aaatgtttta ttattggcta ttttttcttt gtagaaatga taattttgag agttaaattc
     6841 tctaacatat atatggttac aaagtaaggc tatattaaaa attgaatata catgaattga
     6901 tgccagcata aatgatttca aaatataaat taaataataa atctattttt aatatgtaaa
     6961 aatgtcaaat aaattatttt tattgacatg aatatctagt ttatgaaata atattaggag
     7021 cacttatata agtgtttaaa taagagtaga aaaggagcag tgttttatga aaaaaaatac
     7081 taacattaat gagtccggag ttaaacttct taataaataa ggagtttgat gtatagtaac
     7141 tccttattta ttaagtcgtt tacaaaataa tcattatcaa aataaatggt atggtgattt
     7201 aaaaaccaat gacttataaa taaaggagaa aaaataatga atagtaatac atttgaaaga
     7261 ttacaactaa atgaagttaa ggaattaata aaaatacatt gtgtaagttc gcttgggaaa
     7321 aatttaatag ataaattaaa tcctagtggc aatattggag tagtaagaag aaagttagca
     7381 gaaaataaag aggctagaaa gataattgaa aatagcaatc atgtaccact tgaaggattg
     7441 tttaatgcag gttctactat agataaaatt gaaaaaggaa tggtaataga acctattgaa
     7501 cttgtaaaca ttgaagattt tttaagaggt tgtaggaaga tgaaagcttt tatgttagaa
     7561 aaagaatttt attcacctac attaagttct tatgctttaa atataacaga atgtaaaaat
     7621 atagaagatg agataaatta ctgtataaag tctaataaag ttgattctaa tgcaagtaaa
     7681 gaattaaaaa aagtaagacg aaatatagaa attacagaag caaagataaa agatagatta
     7741 aataaattta taacatcaac tgtaaataaa aagtatatac aagagtttat aataagtaaa
     7801 agaaatgata ggtatgtaat accaattaaa tcttcttata aaaatgaagt tgatggtact
     7861 atattagata catcatcaaa aggaaatact gtatttattg aacctattag tgtttcaaat
     7921 ttaagtacag agttaattat gctaaaagct gatgaaacta ttgaagaata caaaatatta
     7981 tcttatttaa cagaacttat atttgagaag ataagtcaga taaaattaaa tatagagata
     8041 ctttcagaat acgatatggt atttgcaaaa gctaaatata gtcaaaaaat aaaaggaatt
     8101 actccaaaaa taaataataa tggctatata aaaataatta aaggtaaaca tccacttcta
     8161 acaggagatg cagtaccttt agattttgaa ataggcaaaa attatagaag tcttataata
     8221 acaggtccta atgcaggagg aaaaactgta acccttaaga ctgttggtct gctaacttta
     8281 atggttcaat gtggatttga tatttctgca aaagaaggta gtgaatttag tgtatttgaa
     8341 aaagtttttg tagatatagg ggataatcaa agtattgaaa atgccttaag tactttttca
     8401 tcacatataa aaaacattgc agagattatg aacttatcaa ataattctac ccttgttctg
     8461 tttgatgaaa taggaagtgg aactgaacct aatgaaggag ctggattagc aatatcatta
     8521 ttagaagagt tttacaaaat gggatgtata actatagcat caactcatta tggagagata
     8581 aaaaagtttg ctacattgca tccagagttt gaaaatgctg gtatgatgtt tgataaagaa
     8641 acgttagaac cattatataa gttaactatt ggaaaatctg aagatagtaa tgcccttttt
     8701 atttctaaaa aaatgggaat aaaaaataga gttttagata gggctaaaga atatgtaatt
     8761 gatagaaact acaatttaga tttagttaaa agaagtaagc tacaatctaa tgttgaagac
     8821 aaaaatagtt ctgaagtcat tttaaactat acagattata atgtagggga taaggtaaaa
     8881 ttactagatg aagatgattt tggaattgta tataagccta tagacaagtt taataatgta
     8941 gaagtattgt ttaatgataa ttttatttca gtaaatataa aaagattaca acttagcata
     9001 aaagcatcag aattgtatcc agaagggtat gatttagata cattatttac taattttaaa
     9061 gaaagaaaat tggaaaaaga tattcaaaga ggttcaaaaa aagctcttaa aaagattcag
     9121 aaagaaatta gagagaatag aaaataaata taaaaaacat aaaagaaagc aatctaaaga
     9181 gcaaatttta tctttagatt gctttcttat taacatatct taataaaccc aatgactaac
     9241 aagattaagc cacttagcca agataagttt aaatttatgt ttttaacaaa catatttcct
     9301 agtaaacaac caaataatac tgcaattata tttgatatta aagaaaatag aataacctgt
     9361 atataattca cacctacaag accactacca aaaccaatag ctaatccatc taaacttaaa
     9421 gcaagtgcaa gtgtaaatgc ttcagcagat ttaagcactt tagatttgct tgtatctaag
     9481 acgtcaggtt caatacttac attaaaattt aatttaaggt ctaacatatt aaaattataa
     9541 ttctttgagt gtatagattt tttcttaaaa taagatttaa aaaagctttc aattagtcta
     9601 gcgcttccta aaaaaaataa gataaagaaa catattagca ttgttatatt ttgaggtaaa
     9661 aagtctctaa ctattgtacc tgcaaaaagc gatattgcca aaatagctga acaaactacg
     9721 ctgataatta tatttgaata taagggaact ttaactttat ttacgccata tccaaagctt
     9781 gtcacaaagg catctataca aagagaagta actaataaaa ttgattcaat cacagtagat
     9841 taacatcctt tctcttaaat tttatactta tattagttat agtattgtaa aaatggataa
     9901 attgttacag ataatccaat aaattgttga taaaatttca acatcataca acacctaaat
     9961 aaaaaaagaa tcatatcata taataatata aaaccaatca taataaagca ggtgaaaata
    10021 atggaacaac atagcaaggt tgggaatttc ggtgatttgt tgaagatttt attagatgaa
    10081 aaagaattat caatgggaga attaagtaaa aaaagtggaa ttgataaatc tactatatct
    10141 cgtatagcta ataataaaca aaagccaaat ataaatcatt tagaaaagat ggcaatacat
    10201 ttaaatatta acctagaaga actgttgaaa gcttcaggat atgaatttaa aaacaataat
    10261 aatcaaatat ttaatacaga tagtgatttt agtaattttg atgatattct tggatttgca
    10321 aatttaatca atgataagaa ctttaatgga aatattgaaa aagagttaag taagtgtaag
    10381 ttgtatgtcc aaacagatga aggtaaaaaa ttactttttg ataattttaa taaaaaaata
    10441 gattctattg aaaaacaagg gcaattcaca gatagattaa gacaaatgta tgctgatttt
    10501 tgtacagacg gacttacaat aaagaaatat ttattgattg gaagtatgct actttacttt
    10561 gttatatcaa cagatgtaat tccagatttc gtgttcccaa taggatttat ggatgattta
    10621 gtggcattaa atatagttac aaaattactt aaaaatgata agtaagtttt agaatcatat
    10681 taaatatata aataaaaata tataaaagtt attatttttt ataagaaaca aattaaaagt
    10741 acaagaaaca gattaaaaat ttaaaactaa gaatctaata tatattttgt aactatttaa
    10801 gtatataaaa taaaataata tccaaactaa tacaaaatag taataattgt tttgtactgg
    10861 aggatattta tgaattgtaa atggatatct aaaatagatg agagaaaaaa atgtgataga
    10921 gaagcagatt cttctggata ttgtatcttt cataaagaaa ataaatctga tgaagagata
    10981 cagttgatgg tagatactct taataaagaa gaaataagtg aatttaatgg atttgtgttt
    11041 gaaaatgaat ttaatgctga agaaatatta acttacaatt ataaaatact ggatttttct
    11101 gaatccatat ttaaacaaaa agcaaatttt aaaaaatata tttttaaaaa gaacattatt
    11161 tttaattata cagaatttaa agataaagtg ttatttaatg gttgtgtatt cttagaaaac
    11221 tgtgatttta atagaaccat atttagtaaa gactatataa atgaccgtat atttgaaaaa
    11281 gtgaaattta aaggttctga tttgatagtt aataaagtag aaaattttcc acgaatggat
    11341 ggaataattt ttagtatgtg tacaaagttt gtgttaaaga atgttgaata tggaaaaagt
    11401 gagtatgagc atggcaagat aaattataga atagctagaa atcaagcaac aaaaattgga
    11461 gaatatgaga tgataggatt ttactattat aaggaaagaa tttatagtag taaaataatg
    11521 aaacgttcta attatcctac tttcagtgac tatttggttg aaaaattttt tgaccaaata
    11581 gcacgctaca cgacaggtta tggagaaaag ccatggaata tattattagt gattatagtg
    11641 ataataagtg tttttgcctt attatactta tttgtaggta tagaaagttc aaatagtaca
    11701 cttgtagctt tagatataaa caatataggt gattactcac taagtgagat tttcagaatg
    11761 tatatggatt tatggtattt tagtatggct acatttagta cagtaggata tggagatatg
    11821 gttgctacaa gtttaattgg gaaagcatta gcaggcatag aagtattttt tggagttaca
    11881 ataggagcaa tttgggcatc tgtgattatt aaaagaatga taaggtaata tctgtttaaa
    11941 tatagatagg tattaattac tagatataaa ttggactaaa tagggaaaat ttaaatataa
    12001 atatacttaa taatccaaga aggtgaaaaa tttgaaaaat gcactaaaaa aaattatatc
    12061 attagccatg attctagttt ttataatacc aaattattca tatgcagatg ataaatctgt
    12121 agacaaatat tcaaaaacat ctatacttat agaccaagaa acaggtagag ttttatatag
    12181 taaagaacct gatatgaaag taccactagc tagtttaagt aagatgatga cttttttact
    12241 tgcaatagaa gctattgaaa ataaagaagt aaaagaaact gataaggtaa agatagacaa
    12301 atctattgca tcagtaaaag ggtcaagtta taaacttaaa gatggtgaag aagtgccact
    12361 tatagaactt atgagaggtc ttatgattgt atcaggaaat gatgcagcaa ttgctatagc
    12421 caaacatatt tgtaaaacaa aggaagcatt tgtggataga atgaataaaa aagcaaaaga
    12481 aataggaatg agcaatacac attttctaaa tccaaatggt cttccaatat acgatttaag
    12541 taatcccaaa aaaccagcaa aagaaaatat atctactgca aaagatatag ctattcttgg
    12601 aaaatacatg tttgacaatt atgaaaaaca agtaacagct ataactgata tggagacata
    12661 tacaaattca gatagaagtt ttgaaaaaag caatacaaat gcattattga gaattatacc
    12721 agaagtagat ggtataaaaa ctggatatac tggcaatgct ggatattgct tatcattttc
    12781 tatggttgta aataaaaatg ataaaaatga gaaaaaacgt agagtaatag gagttgtatt
    12841 aggtactaat cataaaaata aaagaacatc tgcttcatta gctctactaa aatatggaaa
    12901 agaaaatttc aatatgaaaa aagtaattga taaagatagc tttattggta agaaatatat
    12961 aaaaggaatg aaagagctag aggttacatt aaaagcaaaa gatgaacttt atacaatatt
    13021 aaaagatgat gaaagtataa aatcagaagt taagattaaa aatatagagt accctgttaa
    13081 aaaaggcgat actatgggta ttataaaata ttatactagt tcaggtgact tacttggaag
    13141 tgtagatata gtaagtaata atagtataga taatgtaccg ttaaaaacga aattaaaaat
    13201 gaaatttgca aattaaattc aaaaaatggt ataaaataca caaaaaaata taaaaataga
    13261 aaatatggaa aaaatattgt tatttcttca cctctgtgat atactactag aggtgaaaaa
    13321 ataaaataaa tcacacacag cattgggatc cttaaaaggt gcctattaca tggtttttta
    13381 gggagaagat caagctggag gtataaaacc aggaggtaag atatgtcagt aatatcaatg
    13441 aaacaattat tagaagcagg tgttcacttc ggtcatcaaa caagaagatg gaatcctaaa
    13501 atggctaagt atatattcac agagagaaat ggaatatata taatcgactt acaaaaaaca
    13561 gttaaaaaag ttgaagaagc ttacaaattc actaaagaag tagctgaaac aggaaagcca
    13621 atcttattcg taggaactaa aaaacaagct caagatgcga taaaagatga agctgaaaga
    13681 tgtggaatgt actttgttaa tgaaagatgg ttaggtggaa tgttaacaaa ccacaaaact
    13741 ataaaaacaa gaataaacaa attaagagaa ttagaaaaaa tggaagaaga aggcgtattt
    13801 aatgttcttc ctaaaaaaga agttataaaa ttaagagctg aaaaagaaaa actagaaaaa
    13861 tacttaggtg gaataaaaga catgcctgaa ttaccaggtg caatgttcgt agttgaccca
    13921 agaaaagaaa acatagcaat tcaagaagct catagattag gtataccagt agttggtata
    13981 gttgatacta actgtgaccc agaacaatta gactttgcta taccaggaaa tgatgacgct
    14041 ataagagccg taaaattaat aactggtgct atggcaacag ctgtaattga aggtagacaa
    14101 ggtgctgaag aagaagtagc tgaagaccaa gaataataat ttgacaatta aatggtaagg
    14161 gtcgccctta ccatttataa tatggaatag aataagtagg aggaaactta aaaaatggct
    14221 aatataactg ctcaaatggt aaaagagtta agagaaagta caggcgcagg aatgatggac
    14281 tgcaagaaag ctttacaaga agctgaagga aacatggaaa aagcagtaga tttattaaga
    14341 gaaaaaggat tatcaaaagc tgctaaaaaa gctggtagag ttgctgctga aggtctagtt
    14401 gcaatagaaa tgaatgatga taatacagtt gcttctatgg tagaagttaa ctcagaaaca
    14461 gatttcgttg ctaaaaacga agacttcaaa gtgttcgtta aagatgctgc ttgtatggca
    14521 ttagctactg ataaagaaga tatagcttct ttattaggtg aaactcataa agagggaata
    14581 actttacaag aagttttaaa taatagggtt gcaaaaatag gtgaaaaatt agactttaga
    14641 agatttgcta aagttgtaac taatggacag gttgctggat acattcacgg tggcggaaaa
    14701 ataggtgttc ttgttgaaat ggaaacagaa gctagagatg ctaaagtttt agaattaggt
    14761 aaagatgtag ctatgcaagt tgctgctatg aatcctaagt atgtttcaag agatgaagta
    14821 gatgctgaat acatagcaca tgaaacagaa gtattaactc aacaagcttt aaatgaagga
    14881 aaaccagcta atatagttga aaaaatggtt aaaggaagat tagaaaaaga attaaaagag
    14941 gtttgtttat tagagcaaac tttcgttaaa aatccagata taactgttaa acaattagtt
    15001 gctgatgttg ctaaggcagt aggttctgat ataaaagttg ttaaagttgt gagattcgaa
    15061 gttggtgaag gtatacaaaa gagagaagaa aactttgcag aagaagttgc taagcaactt
    15121 aagtaataac caaagagaga acacgcaggt gttctctttt ttaaaaaata gaaaaaatgg
    15181 atatgaagga ttgaaatagg aggtttctga tggataaacc aatgtacaaa agggtattgt
    15241 taaaattaag tggagaagca ttagcaggag aaaaaggttt tggtataaac aatgatgtag
    15301 ttaatgatat tgctattgct ataaaaaaaa tacaagaaat aggtgttgaa gtagctgtag
    15361 tagtaggtgg aggaaacttc tggagaggta gaactagtga aggaatggat agaactactg
    15421 ctgactatat aggaatgtta gctacagtta tgaatgcaat ggcgctacag gatgcactag
    15481 aaaatataga tgttgctaca agagttcaaa ctgctataga catgagacaa attgcagaac
    15541 catatataag aagaagagca gttagacact tagaaaagga aagagttgtt atatttggtg
    15601 caggaacagg aaacccttac tttacaacag atacaactgc tgctttacgt gctgctgaaa
    15661 tggaagcaga agttatcttg ttagctaaaa atgttgatgc agtatatgac aaggatccaa
    15721 aagtacatgc agatgctaag aaatttacag aattaagtta catggaagta atacagaaag
    15781 aattaaaagt aatggattct acagctacat ctttatgtat ggataataaa attccaataa
    15841 aggtatttga gcttacaaca gagaatataa taagagcagt aaaaggcgaa aatataggaa
    15901 ctactgtaaa ataatgaaga atgaaggagg agtaaaatta tgaaactaga aatacataag
    15961 caattagaag agaaaatgaa tgggacaatt gatgctttaa agtttgaatt tggaactata
    16021 agagcaggaa gagccaatgc acaaatgcta gacaaaataa gagttgatta ttatggaact
    16081 ccaactccaa taaatcaaat tggtgctata tctgtaccag agccaagaat attaatgata
    16141 tctccttggg ataaatctgc tatgcatgaa atagaaaaag ctatagctaa ctcagactta
    16201 ggattaaatc catcaaatga tggtgaagtt ataagacttt ctgtacctgc tttaacagaa
    16261 gaaagaagaa aagaattagc taaaaaagct agtaaagctg cagaagactt taaagttaga
    16321 ataagaaatg aaagaagaga cgccaacgag aaaattaaaa agatggaaaa aggcggagaa
    16381 ttaactgaag atgaattaaa aaaagcacaa gatgaagttc aaaaaatgac tgataaattt
    16441 ataaaagaaa tagatacatt attatctaaa aaagaaaagg acataatgga ggtataatat
    16501 tgaaaaaata ttataacctt ttaggtcttc atgtagatga ggtaaaacaa tattttgatg
    16561 agcaagatat aaattatacc ataaaatcta ttgaaggtaa aaaggataaa gataaacttg
    16621 tagttcctag agttataaaa atatctgaga taggtgatag tgtagaactg ataatcacat
    16681 acttttctga ctccttagtt taaggagtca gaacaatatt ggaggcaaaa tatgaataac
    16741 aacattatat atgatataga cttaaataac atacctactc atattgctat tataatggat
    16801 ggaaatggaa gatgggcaaa ggctagattt cttccaagaa ctgcaggaca taaggctgga
    16861 gtagaaacta taagagatat agtaaaagaa tgctcaaaat taggtgttaa gcacttaaca
    16921 ttatatgcat tttcaacaga aaactggaaa agaccgaaac ttgaagttga cacacttatg
    16981 aacttattat ctacatactt aagaaatgaa atagctgaac ttcaccaaaa caatgtaaaa
    17041 gtaactgcta taggagatat aagtgcttta ccaaaaactt gtataagaga attaaactca
    17101 gctaaggaac tgacaaaaaa caatacagga gttaacttaa atttagcatt aaactatgga
    17161 agtagagcag atataaaaaa tgctcttata gatatagtaa aaaattgtga aagtggtaaa
    17221 atagatataa acaatattga tgaagatatt ataaaaaact atttaagcac aaagtctata
    17281 ccagatccag acttggtaat aagaacaagt ggtgaacaaa gattgagtaa ctttttactt
    17341 tgggaggttg cttattctga gttttacttt actgatatac actggcctga ttttaaaaaa
    17401 gaagaactac agaaggctat atacgtatat caaagaaggg atagaagatt tggaggactt
    17461 aagtaaggag attattttat gcttacaaga attattgctt cactagctct ggttccatta
    17521 tttctatttg ttgtatatgg agggattcct ctatatatag ctgagacagc aatagtttat
    17581 attgcattac atgagtttta taaggcattt aaaataaaag atgtaaatcc aatattcatt
    17641 ataggatatt tattttctat ttatttagct gtaaaaaata tttttaattt accattggaa
    17701 tatacttatg cagtaatttt tattttattt ttagcaagta taatttatat gttgatgggc
    17761 aaaaacaatg ttatagatgt ttcgattacg tttctaggag ttttttatat aggagtattt
    17821 ttagatttta taattataac tataaatgaa tttgaaaaag gcagtatata tgtatggctt
    17881 atatttgtga tatcttttat gacagatata tttgcatatt ttagtggtta tttacttgga
    17941 aaacataagt taattcctaa agttagtcct aaaaagacta tagaaggagc tattggaggg
    18001 ataataggaa gtacactttg ttgtatttta tttggatatt tatttggtat tgatttgctt
    18061 caattagcga taataggaag tataggaagt gtaatagctc aattagggga tttatttgca
    18121 tcttctataa agagatatgt agggataaag gattatggaa agataatacc tggtcatggg
    18181 ggtatattag atagatttga tagtgttata ttagttgcac cttttgtata tagtgcgatt
    18241 aaatttttta ttagataaag attggttgga tataaaagag caatctataa ttgctagtaa
    18301 tgaaaatagc tatgtaactt ttaaaagtag agatattctg cagttattag ttacatggct
    18361 attttcattg tttgataatt tagaaaatat taa
//
LOCUS       DAFNNB010000071        18045 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0072, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000071 DAFNNB010000000
VERSION     DAFNNB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0072"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1039
                     /locus_tag="L3M60_002051"
     CDS             <1..1039
                     /locus_tag="L3M60_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6226715.1"
                     /translation="KTRNYPMAINNLAKSEVYLEKIKNQNLKEVAKFDLFISLAMYYA
                     KSNEKSFEQSICYLDRAKNILNIMKNIEEYTMYDLEIIYHQAKIYYIFGYYENALNTN
                     KKLLEKSKNAKNYNYVKLAYKNIYLCFEKIQDYEISMKYFKMYYELKMMYIKARNRNY
                     IDSLNYRHEYIEKEINNMRKVKLDLDKKKYIDHLTSAYNRTFLNDFLEKQEVSEYCTA
                     FMIDVDYFKEYNDTYGHYNGDIALKNITMIIKKYLKKDMLMIRYGGEEFLILSICKDY
                     KKSKIFGRKLCRTVKKFLNNNLTVSIGIDTCKNSSIDIREIIKNADKALYKAKESGRN
                     RCLHYNDFKNF"
     gene            1081..2952
                     /locus_tag="L3M60_002052"
     CDS             1081..2952
                     /locus_tag="L3M60_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6226716.1"
                     /translation="MEDLKRNINKIEYDMLTKFYLNLTDLNYILDYHKDLLNTKLEDD
                     SRVYTLYLISLINYIKKDREASYNYLIESLKYIKKLSLKDDVYVISKIYIYLTLSAIS
                     IRKYDKANNFYFSAKKIIRQNHLNELLVLLNMSLCVEISALYQTKTDVIDLVEEVSFF
                     KNLNNKALVSRANYIFGRVYLYLFNNFIKSMEYLIECLRLAQENNLYSIEAFCKCIIS
                     LCYLESGNNVEAIKYINNVLDVAHNNNSISVTERVSIKIDLIWAYLKEDMNKEAEVIL
                     LKTIKLMKVVEGVYKDYLYSFIFLYSAEIELKKDNCSFEKVNTYLILSKNIYESFGDV
                     FIYNSKLDYIYKLYGDLYIKFGNIDEGISCYIKGLNLVKKSSLNLRKISIFYGLIANG
                     YELKKDFKLAIEYYKSMDWYSDRWEKNNSYKTSQAIHKQYELRQKQESLRLLVDDNKK
                     LENDILKDGLTKLYNRRYLEQQLNLFNGTRDKNMVAILVDIDCFKSYNDNYGHLAGDK
                     VIVKVTDIIRSVFIDITEHIIRYGGEEILILVDSIESISVEEIDNIELYIKRVFKLLE
                     FEGIEHIYSKIKNYITISAGVSIKRCRSRKDVEMLIEDADKNLYKSKKAGRNQYIII"
     gene            2960..4960
                     /locus_tag="L3M60_002053"
     CDS             2960..4960
                     /locus_tag="L3M60_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6226717.1"
                     /translation="MILLQKKIGIIASDIELKERIEELYREDVENGTIIIDILNLDLM
                     ENQGRILVEKGAQAIIGRGGGYSLVIDTVNVPVIPMNMKSTDLLRAIEIAKKYSKKVV
                     LILGDNEVSFDYVGWRNVISTEITEEWFESKYEIRSKVVKYIDQKDEVVIVGGGLACS
                     FARQYGIDSVFATASDESIREAVEYCKKLLDTLGEEKFNNEVLRNILDGIKDGVIAID
                     SNGSIILYNESAKNMLKVERKCALNKYILDVFPKMEWMLDCLHEKEAVEDRKIRNINN
                     LIVNTRTTLIKVDNSTYGVLGIIQDITKLQNLERKIRFDLNQKGLYARYTFDDFLFKD
                     KLTKEFIEEAKKIGKSDYTTLLYGESGSGKEIIAHSIHNISKRKDRPFVAINCATIAE
                     NLLESELFGYEEGAFTGARKGGKRGLFELAHGGTLFLDEINSLSFNIQTKLLRVIEER
                     QIMRIGSDYIIPLDIRIIAATNESLTEKIVMGTFRADLFYRLSSLEINIPPLRDRRED
                     IIPLFNNFVNEVLKDDGLNGINSIDENFVLTKDEIDKLYNYSWPGNVRELKTIAQKYV
                     VTGKIKLRQDRNFKTKQSLSNSEVDKFNSETTASAEVQDESINISKINDGKISIDIKE
                     VNKYVEEKIISMLFAQGLSKNEVAQVLGISRTSLWKKYNKNI"
     gene            5222..6481
                     /locus_tag="L3M60_002054"
     CDS             5222..6481
                     /locus_tag="L3M60_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6226718.1"
                     /translation="MSQSMVGKHAMWPKENDVIFSISGRAQAAEKALGMDNVINATIG
                     ALMDDSGKLITMKTVYEEYKALDNCEIGAYAALEGQPDYLEAVKKVFFRDYLPEGHIR
                     VLASPGGSGAIKLAVWNYTNEGDEVLTSDWFWSPYVSIAEEANRKVVNYQLFDENRRF
                     NFESFKEKFVNIAEKQGRVFTIINTPAHNPTGYSVADDEWDKILDLSKEVAKDKDKKI
                     IFFVDSAYIDFAGDDDVCRKFFKKFSNLPENVLVLVGFSMSKGFTAYGMRMGAIICIS
                     SSEDVAEEFHYSCVHSCRANWSNCNRSAMAVLSNIVNDPKKFKEYEDEKEIYKNMLTR
                     RADVFVKEAERVGLEILPYIAGFFVSIPCDNPKEVCEELTKHNLFAVPLKMGLRFAVC
                     AVSEDKCKKAPSIIKEALESLEVKINS"
     gene            6542..8329
                     /gene="iorA"
                     /locus_tag="L3M60_002055"
     CDS             6542..8329
                     /gene="iorA"
                     /locus_tag="L3M60_002055"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HBN6226719.1"
                     /translation="MKQLMTGNEAIARGAYEAGVKYASAYPGTPSTEILENIATYKDA
                     IVAEWAPNEKVALEAAIGGSIAGARTMASMKHVGLNVAADPIFTYAYTGVNGGMVLIT
                     ADEPGMHSSQNEQDNRMYAKFAKIPLFEPSTSQEAKDMIKEAFEVSEKYDTPVLYRVT
                     TRLCHSKGLVECYDREEVEIKDYVKNAKKMVTVPANAQIRRGVVEERMETLKKFSNET
                     DLNYYEINDTKIGVIASGMCCNFAKEVFGKNASYMKLGFTNPLPYEKIKEFAEKVDKI
                     YVIEENDPFIEEQIKAYGIDCIGKDVIPPYGEMTPDVLRKAIFGKTSDTIEYKSELVT
                     PRPPSFCAGCPHRGFFYELGKRKNLIVGGDIGCYTLGFAPPYNGIDYVVCMGSAFGTA
                     HGAQKVLNMKDNNEKRLVGVLGDSTFFHTGINGLLDVVYNRGNSISVILDNRITGMTG
                     HQENPGSGYTLQGAKTKEVDIEGLVKACGIEHVRVINPNNLKEVNEALDWALAIEDEP
                     SVIITRWPCVLKKFSKEDIEEFNNPFKTKCKVDHDKCIGCKLCLKTGCPALSFDKENK
                     LSNIDRNQCVGCGVCAQVCPKQAIVKEEK"
     gene            8331..8906
                     /locus_tag="L3M60_002056"
     CDS             8331..8906
                     /locus_tag="L3M60_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HBN6226720.1"
                     /translation="MTKSILLVGVGGQGTILASKLLTMGLMEAGYDVKMSEIHGMSQR
                     GGSVSSQVRYGDCVHSPVIEIGGADILVSFEKMEALRWFNYLKPEGKALVNNHRIDSM
                     TVLIGGAEYQESDIDAELNRLNAKVINAADKAEELGNSKIMNVILLGCLVKSMQLESI
                     DWEKIISENVKPKFVELNIKAFHEGMEMVSK"
     gene            8959..10029
                     /gene="buk"
                     /locus_tag="L3M60_002057"
     CDS             8959..10029
                     /gene="buk"
                     /locus_tag="L3M60_002057"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBN6226721.1"
                     /translation="MTYRILAINPGSTSTKIAVYDGEEQILVKTIDHPAEEIAKYNTI
                     QDQFEMRKEAVLNILKENSIDLKSLSAIVGRGGVLPPVKSGAYLVNEEMIDVLRHRPV
                     LEHASNLGAVVAHAISEPLGINSYIYDSVAVDELIDVARISGLCGMDRSSAGHALNTR
                     AMALKYAKDNGKDYKSLNLIVAHIGGGVSIYLHEKGRMVDMLSDDEGPFSPERSGRVP
                     ATKLVAACYSGKYSEREMTKKIRGKGGIVSYLNTVDAREVEKMIAEGNEEAKIIYEAM
                     AYQLAKGIGELATVVDGNVDAIIITGGIAYSEMFTSMVKKKVEFIAPVEIMAGENELE
                     SLAFGTLRVLNGEEEARIYSEN"
     gene            10245..10946
                     /locus_tag="L3M60_002058"
     CDS             10245..10946
                     /locus_tag="L3M60_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226722.1"
                     /translation="MGKMNLKSMARLFSGFFIFAISSVLMINAHVGLMPWDVLHQGLS
                     IKLGITIGQASIMVGVIIVILDAVFGENIGWGTLLNMTFIGIFIDLVIFSGVIPHASN
                     TYIGVLMVVIGIILAAIASFLYLGVCLGSGPRDGLMIALQKKTNKSVRLVRTILEILA
                     LVVGWLLGGSVGIGTLVSALGLGYVLQIVFRIFKFDTKLLKHRFIIDDIREWKEKKSD
                     EHKCKSSIVIKNEQN"
     gene            11006..11512
                     /locus_tag="L3M60_002059"
     CDS             11006..11512
                     /locus_tag="L3M60_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HBN6226723.1"
                     /translation="MELWDLYNADGIKTGNVIKRGNSIEDGYYHLAVEVWILNSNSQI
                     LIQKRSKSKKTLPNMWGMTTGCIVSGEESLEGAIREAKEEIGIDITKDEMKIFRSMTH
                     EDTLWDVYLVKKEYDISKAILQEEEVSDIKWVSTDEIRQLLKEGLFFEYPEIYELLSE
                     IDNNKLNI"
     gene            11685..12707
                     /locus_tag="L3M60_002060"
     CDS             11685..12707
                     /locus_tag="L3M60_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBN6226724.1"
                     /translation="MEDNKGLAHDSSKYKIVSISVVVIILITFWYMLNMVLLTFIMTF
                     IFYNLLVATRKRIKKFSYLNIPDSLIIIVLYALFAMLLVLISYAVVPIIIVQLTELSR
                     VFSDFDVNQFAQSLGPKLYPIVSKLDFNKYISQAGLLIASTATKVGSFGVNILLAFLL
                     SLLLLLEKNEIKKFGDKLSDSKISFIYNSLVFFGKSFVKNFGEVMKVQVMIAFINSVV
                     SMIFLGFMRFPQIWALGFMIFVLGLIPVAGVIVSLIPLTVIAFNTGGITKVFGVLLMI
                     CIVHAIETYILNPKLMSNRTKLPVCFVFIILLVGEHYLGVWGLLIGVPIFMFLMDILG
                     VKFTSR"
     gene            complement(12852..13160)
                     /locus_tag="L3M60_002061"
     CDS             complement(12852..13160)
                     /locus_tag="L3M60_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBN6226725.1"
                     /translation="MKNNNLNCAATNCAYNTSGYCYAGSIKVDGMQATTTENTYCASF
                     EDKYTSGITSRSNETNQVDTDNIHCEAVKCKYNKNELCKAEKVHINEKNASCETFEMK
                     "
     gene            complement(13220..13648)
                     /locus_tag="L3M60_002062"
     CDS             complement(13220..13648)
                     /locus_tag="L3M60_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226726.1"
                     /translation="MNNKLHELEKNLPETSCSFCTHLSFKGPSLDYRYDIKCVISDTK
                     PDFRGCCEYFEPEYTELNTGDLDNLYINFLETCLKVNYNDYINSMYWKLFKERTLLEN
                     NFTCSICGSTENVDVYHVNKNLGRETSKDVVVKCNKCICK"
     gene            complement(13869..15326)
                     /locus_tag="L3M60_002063"
     CDS             complement(13869..15326)
                     /locus_tag="L3M60_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBN6226727.1"
                     /translation="MTTFLIGLAILLIGGALYGAYCEKVFGPDDRKTPALAQSDGVDY
                     VPMKKWKNSLIELLNIAGTGPILGPIQGILFGPIAFILIPIGCVFGGALHDYMSGMIS
                     IREKGAQMPSLISRFLGNKVFQVYNIFLCLLMLLVGAVFIYTPGDLVVTQILNMKSTI
                     NNPVVWIVYGIIFLYYLCATLFPIDKIIGKIYPIFGAILLLSAAGVGVGIFTQGYDLA
                     NLSLANWKGIHPDGIPLIPTFFVTVACGIVSGFHSTQATLIARSVSNEKEGKTTFYNM
                     MILEGLIAMIWAAAAMGIYNKGIPKELIGSPDVIGLVARDLLGSIGGIIAIIGVIVLP
                     ITSGDTALRSLRLMLADYFHYDQKEKKHRVILSICIFIPVIAILIFAKLSASGFNILW
                     RYFSWSNQTIAIFAFAMITVYLIVKEKNYIISLIPGMFYSFVIFSYIFNAQIGFNLNM
                     NISYVLAAIFTVLYAILTVHSGRKLKSRADTKLAD"
     gene            15744..16658
                     /gene="nadA"
                     /locus_tag="L3M60_002064"
     CDS             15744..16658
                     /gene="nadA"
                     /locus_tag="L3M60_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBN6226728.1"
                     /translation="MDKDLTYQIKELKKEKNAIILAHFYQPPEIQELADAVGDSYYLS
                     EIARDCKEEVVVFCGVRFMGESAKILSPEKTILMPVSNAGCAMADMVDEEGVIKLKQQ
                     YPNALVVCYINSTAKVKAHCDVSVTSSSAIKILENIDNKEIIFLPDKNLGGYIAEQFP
                     DKNFIFWDGYCKYHNNIRAEEIIELKDKYKNAEVLVHPECKKEIRDLGDYVGSTSGII
                     KYATNSKNKDFIIATEEGILHELKKNNPNKNFYIPGGKILCTDMKKTTLENLYITLKN
                     MENEVIVEDEIMEKALNSLLNMHKLAEG"
     gene            16671..17972
                     /locus_tag="L3M60_002065"
     CDS             16671..17972
                     /locus_tag="L3M60_002065"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HBN6226729.1"
                     /translation="MNLEQDVLIVGSGVSGLYCALNLDKSLNVLVVSKSTIENNNTYL
                     AQGGISTARNIDDIESFVEDTMRAGQYKNRVEAVQVLADESIENVGQIVEYGMPLDKE
                     NGEIDYTREGAHSVNRIVHSKDNTGEVVFKTLLKEAKTRENITLIEDAYLLDILKDGN
                     KCIGARIFKSKKEIHVFSKIVVLATGGIGGLFKNSTNQRHLTGDGIAIALRNNIKIEN
                     LEYIQIHPTAFYEENNEGRRMLISESLRGEGAKLLNKNKERFVDELLPRDVVSKAIFE
                     QMEKDKLPYVYLDATHLDSEYLINRFSFIYNECLARGTDITKECIKVSPAQHYFMGGI
                     HVDLDSKTSMENLYAVGEISCTGVHGANRLASNSLLEGLVFSKRAAKNINSVIDNVKV
                     KFVDIPDMDIDIEQVKKENKILVIKEIERTSEDFGDELFDY"
     gene            17953..>18045
                     /locus_tag="L3M60_002066"
     CDS             17953..>18045
                     /locus_tag="L3M60_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HBN6226730.1"
                     /translation="MNYLIIDRMIQDALIEDVPSEDITTNSIVDE"
BASE COUNT         7095 a         1886 c         3220 g         5844 t
ORIGIN      
        1 aaagactaga aattatccta tggcaataaa taatttagca aaatccgaag tatatttaga
       61 aaaaattaaa aatcaaaact taaaagaagt agcgaagttt gatttattta taagcttagc
      121 tatgtattat gcaaaaagta atgaaaaatc ttttgaacaa tcaatctgct atttagatag
      181 agctaaaaac atattaaata taatgaaaaa tatagaagaa tatacaatgt atgatttaga
      241 aataatttat caccaggcaa agatatatta tatctttgga tattatgaga acgctttaaa
      301 cacaaataaa aaacttcttg aaaaatctaa aaatgctaaa aattataatt acgtgaagtt
      361 agcatataag aatatatatt tgtgctttga aaagattcaa gattatgaaa taagtatgaa
      421 atactttaaa atgtattatg agttaaaaat gatgtatata aaagctcgaa atagaaatta
      481 tatagattct ttaaattata gacatgagta tatagaaaaa gaaataaata acatgagaaa
      541 agtaaaactt gatttagata aaaaaaagta tattgaccat ttaacaagtg catataatag
      601 aacttttttg aacgattttt tagaaaaaca agaagttagt gaatattgta cagcatttat
      661 gatagatgta gattacttta aagaatacaa tgatacttat ggacactata atggagatat
      721 tgctcttaaa aatataacta tgataataaa aaaatatcta aaaaaagaca tgcttatgat
      781 aaggtatgga ggagaagaat ttctgatatt gtctatatgt aaagattata aaaaaagtaa
      841 aatatttgga agaaaattat gtaggactgt aaagaaattt ttaaataata atttgacagt
      901 tagtattggc attgatacat gtaaaaatag tagtatagat ataagagaaa taattaaaaa
      961 tgctgataaa gctttatata aagcaaagga aagtggtaga aatagatgtt tgcattataa
     1021 tgattttaag aatttttaac tgttgaaata ggattggtga tttacttgaa aaggagttta
     1081 atggaagatt taaaaagaaa tataaacaag atagaatacg atatgttgac taaattctat
     1141 ctaaatttaa cagacttaaa ctacatcttg gattatcaca aagacctttt aaatacaaaa
     1201 ctagaagatg atagtagagt atatacttta tatttaatta gtcttataaa ctatataaaa
     1261 aaagatagag aagcatcata caactatttg atagagtcat taaagtatat taagaaattg
     1321 tcattaaagg atgatgtata tgtaatatct aaaatttata tttatcttac tttatcagct
     1381 atttctataa gaaaatatga taaggcaaat aatttttatt tctcagcaaa aaaaattata
     1441 agacaaaacc atttaaatga gttgttggtc ttgttaaata tgagtttatg tgtagaaata
     1501 agtgctttat atcagaccaa aacggatgta atagatttag tagaagaagt gtcttttttt
     1561 aaaaatttaa ataataaagc tcttgtaagt agggcaaatt atatatttgg gagagtttat
     1621 ctatatttat ttaataactt tataaaaagt atggagtact taattgaatg cctaagactg
     1681 gctcaagaaa ataacttata ctctatagag gctttttgta aatgtataat aagtttgtgt
     1741 tatttagaga gtggaaataa tgtagaagct ataaaatata taaataatgt attagatgtt
     1801 gcacacaata ataactcaat atcagtaact gaaagagtgt ctataaagat agaccttata
     1861 tgggcctatt taaaagaaga tatgaataaa gaagcagaag ttattctatt aaagactata
     1921 aaattaatga aagttgttga aggtgtatat aaggattatt tatattcatt tatattttta
     1981 tattcagctg aaattgagtt gaagaaagat aattgtagtt ttgaaaaggt aaatacctat
     2041 ttaattttat caaaaaatat atatgaaagt tttggagatg tttttatata taattctaaa
     2101 ctagattaca tatataagtt gtatggagat ttatatataa aatttggcaa tatagacgaa
     2161 ggtataagtt gttatataaa aggtttaaat ttagttaaaa agagttcatt aaatcttagg
     2221 aaaatctcta tattttatgg gctgatagca aatggatatg agctaaaaaa agattttaaa
     2281 cttgcaatag aatattataa aagtatggat tggtattctg atagatggga aaaaaataat
     2341 tcctataaaa catctcaagc aattcacaaa caatatgaac ttagacaaaa acaagagagt
     2401 ttaagattat tagtggatga taataagaaa ttagaaaatg acattttaaa agatggactt
     2461 actaaactat ataatagaag gtatttggaa cagcagttga atttattcaa tggaactaga
     2521 gataaaaata tggttgcaat actggttgat atagattgtt ttaaaagtta taatgataat
     2581 tacggtcatc tagcaggaga taaagttata gttaaggtaa ctgatattat aagaagtgta
     2641 tttatagata ttactgaaca cataatcaga tatgggggag aagaaattct aatactagta
     2701 gattctatag aatcaataag tgttgaagag attgacaaca tagaattata tattaaaaga
     2761 gttttcaaac tgcttgaatt tgagggtatc gaacatatat attctaaaat aaaaaactat
     2821 ataactataa gtgctggagt aagtataaaa agatgtcgta gtagaaaaga tgttgagatg
     2881 ttaattgaag atgcagataa gaacctatat aaatcaaaaa aagcaggtag aaatcaatac
     2941 attataatct aaaattgagg tgattttatt gcaaaaaaaa ataggaatta tagcctctga
     3001 tattgaatta aaagaaagaa tagaagaatt atatagagaa gatgtagaaa atgggactat
     3061 aataatcgac atacttaacc tagacttaat ggaaaatcaa ggtcgtatac ttgttgaaaa
     3121 aggtgctcaa gccataattg gtaggggtgg aggttatagt ttagtaatag atacagtaaa
     3181 tgtacctgtt ataccaatga atatgaagtc aactgacctt ttaagagcaa tagaaatagc
     3241 aaagaaatat tctaaaaaag tagtattaat tcttggagac aatgaagttt cttttgacta
     3301 tgttggctgg agaaatgtca taagtactga aataactgaa gaatggtttg aatctaagta
     3361 tgagataaga agtaaagttg taaaatatat agaccaaaaa gatgaagttg taatcgtagg
     3421 tggaggtttg gcatgttctt ttgcaaggca atatggaatt gatagtgtat ttgcaactgc
     3481 tagtgatgaa tctataagag aagctgtaga atattgtaaa aagttgttgg atacattagg
     3541 tgaagaaaaa tttaataatg aagtgttacg aaatatactt gatggtataa aagatggagt
     3601 aattgctata gatagtaatg gtagtataat tttgtacaat gaaagcgcaa aaaatatgct
     3661 taaagtagag agaaaatgtg cgcttaataa atacatttta gatgtatttc ctaaaatgga
     3721 gtggatgtta gattgtttac atgaaaaaga ggctgtagaa gatagaaaaa taagaaatat
     3781 aaataattta attgttaata ctagaacgac attgataaaa gttgataata gtacttatgg
     3841 agttttagga ataatacaag atataaccaa gctacaaaac ttagaaagaa aaataagatt
     3901 tgacttgaat caaaaaggtc tttatgctag atatactttt gatgattttt tatttaaaga
     3961 taaattaact aaagaattta ttgaagaagc caaaaaaatt ggaaaaagtg attatacaac
     4021 tttactttat ggagaaagtg ggtcaggtaa agaaattata gcccatagta tacacaatat
     4081 aagtaaaaga aaagatagac cctttgttgc cataaactgt gctactatag cagaaaattt
     4141 acttgaaagt gaactttttg gatacgaaga aggtgcattt actggtgcta gaaagggtgg
     4201 aaaaagagga ttgtttgaac ttgctcatgg aggtactctt tttctagatg aaataaatag
     4261 tctatctttt aatattcaga caaaactact aagagtaata gaggaacgtc aaataatgag
     4321 aattggttct gattatataa tacctctaga tataaggatt atagctgcaa cgaatgaatc
     4381 tttaaccgaa aaaatagtta tgggtacctt tagggcagac ttattttata gattgagtag
     4441 tttagagata aacatcccac ctttaagaga cagaagagaa gatataatac ctttgtttaa
     4501 caattttgta aatgaagtat tgaaggatga tggtctcaat ggaataaaca gtatagatga
     4561 aaattttgtt ttgactaaag atgaaattga taagctatat aattatagtt ggcctggaaa
     4621 tgtaagagag ttaaagacaa tagctcaaaa atatgttgtg acaggtaaaa ttaaattgag
     4681 acaagatagg aattttaaaa ctaaacaatc actttctaac tctgaagtag acaaatttaa
     4741 ttctgaaacg acagcaagtg cagaagtcca agatgagtct ataaatataa gtaaaataaa
     4801 tgatggtaag ataagtatag atataaaaga agtaaataag tatgttgaag aaaaaataat
     4861 aagtatgctt tttgctcagg ggctttctaa aaatgaagtt gcacaagttc ttggaataag
     4921 taggacatct ttatggaaga aatataataa aaatatttaa atataattac atataaatta
     4981 ttttgaagta atgttaattt ttttaaacaa gtgttaagaa aaattaacat tatttttttg
     5041 caaattttta tttttttaga caagcatata tatttataag aaaaatagct aaaacactta
     5101 atttaataat gaaaaaaata ataattctaa acaaaatcag ttttacataa agtggaattt
     5161 attggcataa taattgctta atatataatg caagagtaaa tacaatttta ggggtgatgg
     5221 tatgagtcaa agtatggttg gtaagcatgc aatgtggcca aaagaaaatg atgttatatt
     5281 tagcatatct ggtagagctc aagcagcaga gaaggctctc ggtatggata atgtaataaa
     5341 tgcaacaata ggcgcgctaa tggatgattc aggaaaatta ataacaatga aaactgtata
     5401 tgaagaatat aaagcattag ataactgtga gataggtgca tatgcagctc ttgaaggtca
     5461 accagattac ttagaagcag ttaaaaaagt atttttcaga gattacttac cagaaggaca
     5521 tataagagta ttagcttcac caggaggaag tggtgcaata aaattagctg tatggaacta
     5581 tactaatgaa ggagatgagg tattaacatc tgattggttc tggagtccat atgtaagtat
     5641 agctgaagag gctaatagaa aagtagttaa ttaccaactt tttgatgaaa atagaaggtt
     5701 taattttgag tctttcaaag aaaaatttgt aaatatagca gaaaaacaag gtagagtttt
     5761 tacaattata aacacaccag cacacaatcc aactggatat agtgtcgcag atgatgagtg
     5821 ggataaaata ttagacttat caaaagaagt tgcaaaagat aaagacaaaa agataatatt
     5881 tttcgtagat tcagcatata ttgactttgc aggtgatgat gatgtatgta gaaagttttt
     5941 taagaaattt tctaatttgc ctgaaaacgt tctcgtattg gttggcttca gtatgtcaaa
     6001 aggatttaca gcttatggta tgagaatggg ggctataatt tgtatatctt caagtgaaga
     6061 tgtagctgaa gagttccatt attcatgtgt tcattcatgc agagcaaatt ggtcaaattg
     6121 taatagaagt gccatggcag tattatctaa cattgtaaat gaccctaaaa aattcaaaga
     6181 atatgaggat gaaaaagaaa tatacaaaaa catgcttact agaagagcag atgtatttgt
     6241 aaaagaagct gaaagagtag gattagaaat tcttccatat atagcaggtt tctttgtaag
     6301 tataccatgt gataatccaa aagaagtatg tgaagaatta actaaacata atttatttgc
     6361 agttccatta aaaatgggat tgagatttgc agtttgtgca gtttctgaag ataaatgtaa
     6421 gaaagcacca agtattataa aagaagcact tgaaagttta gaagtaaaaa taaatagtta
     6481 atttcattga acaaattggg tggactatga atttaataat tgtagatgga gggaatttta
     6541 tatgaaacaa ttgatgacag gtaatgaagc aattgcacgt ggagcctatg aagctggtgt
     6601 taaatatgca tctgcatatc cagggactcc aagtacagaa atattagaaa atatagcaac
     6661 atataaagat gcaatagttg ctgaatgggc tccaaacgaa aaagtagcac ttgaggctgc
     6721 tatagggggt tcaattgcag gagcgagaac aatggcttct atgaaacatg ttggcttgaa
     6781 tgttgcagct gatccaatat ttacttatgc atatacagga gtaaatggtg gtatggtact
     6841 tattacagca gatgaaccgg gaatgcattc atctcaaaat gagcaagaca ataggatgta
     6901 tgcaaaattt gcaaagatac ctttatttga accatcaact agtcaagaag ctaaagatat
     6961 gataaaagaa gcttttgaag ttagtgaaaa atacgataca ccagttttat atagagttac
     7021 aacaagactc tgtcattcaa agggattagt ggagtgctat gatagagaag aagttgaaat
     7081 taaagattat gttaaaaatg ctaagaaaat ggtaactgtt ccagcaaatg ctcaaataag
     7141 aagaggcgtt gttgaagaaa gaatggaaac acttaaaaaa ttctcaaatg aaactgattt
     7201 aaactactat gaaataaatg atacaaaaat aggtgttata gcttctggta tgtgttgtaa
     7261 ttttgccaaa gaagtctttg ggaaaaatgc atcatatatg aagctaggat tcacaaatcc
     7321 attaccttat gaaaaaataa aagaatttgc agagaaagtt gataaaatct atgttataga
     7381 agaaaatgac ccatttatag aagaacaaat aaaagcatat ggaatagatt gtattggaaa
     7441 agatgtaata ccaccatatg gagaaatgac accagatgtt ttaagaaaag ctatatttgg
     7501 aaaaacaagt gatactatag aatacaaatc tgaattagta acaccaagac caccaagttt
     7561 ctgtgcagga tgtccacata gaggattttt ctatgaactt ggaaagagaa aaaatttaat
     7621 agttggtgga gatataggtt gttatactct tggatttgct cctccatata atggaataga
     7681 ttatgttgtt tgtatgggtt ctgcttttgg aactgcacat ggagctcaaa aagttcttaa
     7741 tatgaaggat aataatgaaa aaagattagt aggtgtactt ggcgattcaa cattcttcca
     7801 tacaggaata aatgggcttt tagatgttgt ttacaataga ggaaattcta tttcagttat
     7861 attagataat agaataactg gaatgacagg tcaccaagaa aatccaggtt caggatatac
     7921 tttacaaggt gctaaaacta aagaagttga tatagaagga ttagttaaag cttgtggaat
     7981 agaacatgta agagttataa accctaataa tttaaaagaa gtaaatgaag cgctagattg
     8041 ggctttagct atagaagatg aaccatcagt aataataaca agatggccat gtgtacttaa
     8101 aaaattctct aaagaggata tagaggaatt taataatcca tttaagacta agtgtaaagt
     8161 tgaccatgat aaatgtatag gatgtaaatt atgtttgaaa actggatgtc cagcactatc
     8221 atttgataaa gagaataagt tatcaaatat agatagaaac cagtgtgtag gatgtggagt
     8281 atgtgcacaa gtttgtccaa aacaagcaat agttaaggag gaaaaataat atgactaaga
     8341 gtatactttt ggtaggtgta ggaggtcaag gaactatact tgctagtaaa ttattaacta
     8401 tgggattgat ggaagctgga tacgatgtaa aaatgagtga aatacatgga atgagtcaaa
     8461 gaggaggttc agtttcctca caagttagat atggagattg tgttcattct ccagttatag
     8521 aaattggtgg agcagatata ttagtatctt ttgaaaaaat ggaagcttta agatggttca
     8581 attatctaaa accagaagga aaagctttag taaataatca taggatagat tctatgactg
     8641 ttttaatcgg aggagctgag tatcaagaaa gtgatataga tgcggagtta aatagattaa
     8701 atgcaaaagt tataaatgca gctgataaag ctgaagaact aggaaattca aaaataatga
     8761 atgttatttt attaggatgt ctagttaaat ctatgcaatt agagtctatt gactgggaaa
     8821 aaattataag tgaaaatgtt aagccaaaat ttgttgaatt aaacataaaa gctttccatg
     8881 aaggtatgga aatggttagc aaatagttta taatagatat aaatagacta tagaaattaa
     8941 ggtgaaagga agaaaattat gacttacaga atattagcca taaatccagg ttctacttct
     9001 acaaaaatag cagtatatga tggagaagaa caaattcttg tgaagacgat agaccatcca
     9061 gctgaagaga ttgcaaaata taatactata caagaccagt ttgaaatgcg taaggaagca
     9121 gttttgaata ttcttaaaga aaatagtata gatttaaaat ctcttagtgc aatagtagga
     9181 agaggtggag ttttaccacc agtaaaatca ggagcatatt tagtaaatga agaaatgatt
     9241 gatgtactta gacatagacc agtgcttgaa cacgcttcca acttaggtgc tgttgtagca
     9301 catgcaatat cagaacctct tggaataaat tcatatattt atgattctgt tgcagtagat
     9361 gagcttatag atgtagcgag aatatctggg ctttgtggaa tggatagatc aagtgcaggg
     9421 catgcattaa atactagagc aatggcttta aaatatgcta aggataatgg aaaagattat
     9481 aagagcttaa atttaatagt agctcatatt ggtggaggag taagtattta tcttcatgaa
     9541 aaaggaagaa tggttgatat gttatctgat gatgaaggac cattttctcc agaaaggtca
     9601 ggaagagtac ctgccacaaa attagtggct gcctgttatt caggtaaata ctcagaaaga
     9661 gaaatgacta aaaagataag aggtaaaggt ggtatagttt catacctaaa tactgtagat
     9721 gctagagaag ttgaaaaaat gatagcagaa ggaaatgaag aagcaaaaat tatttatgaa
     9781 gcaatggctt atcagttagc aaaaggtatt ggagagttag caactgtagt agatggaaat
     9841 gtagatgcta taattataac aggtggaatt gcatattctg aaatgtttac ttcaatggtt
     9901 aaaaagaaag ttgagtttat agcacctgta gaaattatgg cgggagaaaa tgagttggaa
     9961 tcacttgctt ttggaacttt aagagtacta aatggagaag aagaagctag aatttatagt
    10021 gaaaattaat aagaaaaact aaaaatgttt atatactaaa aaagtggtat atccgttttt
    10081 tatacacgaa agcttagagt ataggtgaaa atgtataaag tttggattgc cactttttat
    10141 aattaaaatt ttatcttaag ctaataaata attctgtaaa atttcgtatt tatatatatt
    10201 ttattggtat ttattgttat tattattatg aggagtgatt tttgatggga aaaatgaatt
    10261 taaaaagtat ggcaagatta ttttcaggtt tttttatatt tgcaatttct tctgtgctta
    10321 tgattaatgc acatgtaggt cttatgcctt gggatgtact tcatcaaggt ttatctataa
    10381 agctaggtat aactataggt caggcttcta taatggtagg agtcataatt gttatattgg
    10441 atgcagtttt tggtgaaaat ataggatggg gtactttact caacatgacg tttataggaa
    10501 tttttattga tttagtaata ttttctggag tgatacctca tgcaagtaac acttatatag
    10561 gtgtgcttat ggttgttata ggaattattt tggcagctat agcaagcttt ctatatttag
    10621 gtgtttgtct aggaagtgga ccgagagatg ggcttatgat tgctcttcag aaaaaaacta
    10681 ataaatctgt acgtcttgtt agaacaattc ttgaaatact agcattggtt gttggttggt
    10741 tattaggagg ttctgtaggt attggtactc ttgtaagtgc tttaggttta ggatatgtat
    10801 tacaaatagt gtttagaata tttaaatttg atacaaaatt actaaaacat agatttatta
    10861 tagatgatat aagagaatgg aaggaaaaga aaagtgatga acataaatgt aaatcaagta
    10921 ttgtaataaa aaatgagcag aattaattat gctaaaatat atttatagtt tatcaaagtt
    10981 atagtaaagg caaggagact ttgaaatgga attatgggat ttatataatg ctgatggaat
    11041 taaaacagga aatgttataa aaagaggtaa ttctattgaa gatggttatt accatttggc
    11101 agttgaagtg tggattttaa atagtaactc tcaaatttta attcagaaaa ggtctaaaag
    11161 taaaaagaca ttgccaaata tgtggggaat gacaacgggg tgtattgttt ctggggaaga
    11221 gagtttagaa ggagcaattc gtgaagcaaa ggaagaaata gggatagata taactaaaga
    11281 tgagatgaag atttttagaa gtatgactca tgaagatacc ctttgggatg tatatttggt
    11341 gaaaaaggaa tatgatatat ccaaagccat tttacaggag gaagaagtaa gtgatataaa
    11401 gtgggtaagc acagatgaaa taagacaatt actaaaagaa ggtttatttt ttgaatatcc
    11461 agagatatac gaattgctat ctgagataga taataataaa ttaaatatat gatgtagagc
    11521 agatttcagt ataagtatga ttaaaaattt atcttctcta aattttaata ttgttttatg
    11581 aaaattaata agttattttt agtatttttc tacttgtttt ttaagattaa aataaaaaag
    11641 ggcggtcatt gtaaagtcat aaaaatttgg ggaggcttta aactatggaa gataacaaag
    11701 gattggcaca cgacagttca aagtataaaa ttgtttctat atctgttgta gtaattattc
    11761 taattacatt ttggtatatg ttaaatatgg tgcttttaac attcattatg acttttattt
    11821 tctataactt gcttgtagcc acaagaaaac gaattaagaa attttcttat ttaaatattc
    11881 cagattcatt gataattata gtgttatatg cactatttgc catgttgttg gtattaatta
    11941 gttatgcagt tgtaccaata attatagttc aacttacaga gttgagtagg gtttttagtg
    12001 attttgatgt aaatcaattt gctcaatctt taggaccaaa actgtatcct attgtttcaa
    12061 agctagattt taataaatat atttcacaag cagggctact catagcatca acagcgacaa
    12121 aagttggaag ttttggagta aatatattat tagctttttt gttgagttta cttcttttac
    12181 ttgaaaaaaa tgaaattaaa aagtttggag ataaactttc tgatagtaag atttctttta
    12241 tttataattc gcttgtattt tttggaaaga gttttgttaa aaattttggt gaagtaatga
    12301 aagtgcaagt gatgattgcc tttataaatt ctgttgtttc tatgattttt ttaggtttca
    12361 tgagattccc tcaaatatgg gccttaggat ttatgatttt tgtgttaggt cttattccag
    12421 tagcaggagt tattgtgtct ttgattccat taactgtaat agcatttaat acaggaggaa
    12481 taacaaaggt ttttggagtt ttacttatga tttgtatagt gcatgcaata gaaacataca
    12541 tattgaaccc taagttgatg tcaaatagaa ctaaacttcc agtttgtttt gtttttatca
    12601 ttttacttgt aggagaacac tacttaggtg tatggggatt actaattgga gttccaatat
    12661 ttatgtttct tatggatatt ctaggtgtta aatttacttc tagataatat attaaaataa
    12721 taatttattt ttatatatat tatgaattgt tttaaaaaag aacctataaa tagacatcat
    12781 taataaagat atctatttga taggttcaat tttgattttt ataaatacta taaacttaaa
    12841 tgaaaagaaa tttatttcat ttcaaaagtt tcacaacttg catttttttc atttatatga
    12901 actttttcag ctttgcaaag ttcattttta ttgtatttac attttacagc ttcacaatga
    12961 atgttatctg tatcaacttg gttagtttca ttagaacgac tagttattcc agatgtatat
    13021 ttgtcttcaa aagaagcaca ataagtattt tctgtagtag ttgcttgcat tccatctact
    13081 tttatactac cagcataaca gtatccacta gtattataag cacaattagt agcagcacaa
    13141 tttaaattat tatttttcat aaataacctc ctaagtttta tttaaactta ttatgtgaag
    13201 ttatctaaat tttatacact tatttacaaa tacatttatt acatttaaca actacatctt
    13261 tggaagtttc tcttcctaaa tttttattaa catgatatac atcaacgttt tcagttgaac
    13321 cacatataga acatgtaaaa ttattttcta gtagagttct ttctttaaat aacttccaat
    13381 acatagaatt gatgtaatca ttataattta ccttgaggca tgtctcaagg aagtttatgt
    13441 atagattgtc taaatctcct gtatttaact ctgtatattc tggttcaaaa tattcacaac
    13501 atcctctaaa atcaggcttt gtatctgaaa taacacattt aatatcatat ctatagtcta
    13561 ggctaggccc cttaaatgaa agatgtgtgc aaaaagaaca agatgtttct ggtagatttt
    13621 tttcaagttc atgtaattta ttattcataa gtattcctcc aaaaaattta ttttaagtat
    13681 aaatgaatag attattttaa aacttttatt tatttatatt agaaatatag gataaatatt
    13741 ccaataagga gtaaatactt aaatgtttat ttctgtatta aataaaaaag aggcaaatct
    13801 aacttgagat tgcctacttt tttattactt tttaataaaa tttaatctaa atattatttt
    13861 atttaccttt agtcagctaa tttagtatct gctctgcttt ttaactttct accagagtgt
    13921 acagttaaaa tagcatataa tacagtaaat atggcagcta aaacgtatga tatattcatg
    13981 tttaagttaa atccaatttg agcattaaat atataactaa atataacaaa agaataaaac
    14041 atacctggaa ttaagcttat aatataattc ttttctttta ctataaggta aactgtaatc
    14101 attgcaaacg caaatatggc gatagtttga ttactccaag agaagtatct ccacaatata
    14161 ttaaatccag aggcactaag ttttgcaaat ataagaatgg ctatgacagg aataaatata
    14221 cagattgata atataacacg atgtttcttt tccttttggt cataatgaaa gtaatctgca
    14281 agcattaatc ttaaagatct taaagctgta tctccagaag ttattggtaa aacaattaca
    14341 cctattatag ctatgatacc accaatagag ccaagtaaat ctcttgcaac taaacctatt
    14401 acatcaggag aacctattaa ttccttaggt attcctttat tgtagatacc catagcagca
    14461 gcagcccaaa tcattgcaat tagaccttct aaaatcatca tattataaaa agtagtcttt
    14521 ccttcttttt catttgaaac tgatcttgct ataagagtag cttgagttga gtggaatcca
    14581 gaaacgatac cacaagcaac tgtaacaaag aatgtaggaa taagaggaat cccatcagga
    14641 tgaatacctt tccaatttgc aagacttaaa ttagccaaat cataaccttg tgtaaagatt
    14701 ccaactccaa caccagcagc agataaaagt aggatagctc caaatattgg atatatcttt
    14761 cctatgattt tatcaattgg gaatagtgtt gcacataagt aatatagaaa gataattcca
    14821 tagactatcc agacaactgg attatttata gtcgatttca tatttaaaat ttgagtaaca
    14881 actaaatctc caggtgtata aataaaaact gcaccgacta aaagcattaa aagacaaagg
    14941 aatatattat agacttgaaa tactttatta cctaaaaatc tacttataag agaaggcatt
    15001 tgtgctcctt tttctcttat actaatcatt ccactcatat agtcatgtaa agctcctcca
    15061 aatacacagc caataggtat tagtatgaat gctattggac caaacaatat accttgtata
    15121 ggtcctagaa ttggtcctgt accagctata ttaagcaact caattagact atttttccat
    15181 tttttcattg gaacatagtc tactccatca ctttgagcaa gtgcaggtgt ttttctgtca
    15241 tctgggccaa atactttttc acaataagca ccgtataaag cacctcctat tagtaatata
    15301 gccaagccga ttaagaatgt tgtcataata tcaccccatt tataaaattt tttggtcttt
    15361 gtatacatat tatctaaatg tgtagattta tatgacattt aattctggac aacctacaaa
    15421 ttctataaag tattaaaact aatataattt cagaaataaa tacatataca taaccatgta
    15481 cttagtatac aggtatttga tattattttc aatattatat caataaagaa tataaacaaa
    15541 attctgataa tattttacaa aaaaattaaa aaatataaaa aatagtttaa atagatgagg
    15601 aaaaaaggaa aatgaattga tactgtaaat gtctatatct taacaatatt aagaggttta
    15661 aaattcttga agaaagaata gtcaagtgat ataatgagtt tacaggtgta tatacactag
    15721 tacatataga aagcggtgca attatggata aagatttaac atatcaaatc aaagagctaa
    15781 agaaagaaaa aaatgcaata atacttgccc atttttatca accaccagag atacaagaat
    15841 tagcagatgc agttggtgat tcatattatt taagtgagat tgctagagat tgtaaagaag
    15901 aagtggttgt attttgtgga gtaaggttta tgggagaaag tgctaagata ttatcacctg
    15961 aaaaaactat attgatgcca gtaagtaatg ctgggtgtgc tatggcagat atggtagatg
    16021 aagaaggcgt gataaaatta aaacaacagt atccaaatgc tttggttgta tgttatataa
    16081 actctacagc taaagttaag gcacattgtg atgtttctgt tacatcttct agtgcaatta
    16141 aaatattaga aaacatagat aataaagaaa ttatattcct tccagataaa aatttaggag
    16201 gatatattgc agaacaattt ccagataaaa actttatatt ttgggacgga tattgtaaat
    16261 accacaacaa tataagggct gaagaaataa ttgagttaaa agataagtat aaaaatgcag
    16321 aagtattagt acatccagaa tgtaaaaaag aaataagaga tttaggagat tatgtaggta
    16381 gtacatcagg aataataaaa tatgctacta atagtaaaaa caaagacttt ataatagcaa
    16441 ctgaagaagg tatattacat gaattaaaga aaaataatcc taataaaaat ttttatatcc
    16501 ctggtggaaa aatactatgt acagatatga aaaagacaac attagaaaat ttatatatta
    16561 cacttaaaaa tatggaaaat gaagttattg tagaagatga aattatggaa aaggctttaa
    16621 attcactgct taacatgcac aaattagcag aaggatagag gtatagactt atgaatttag
    16681 aacaagatgt tttaatagtt ggaagtggag tttcaggatt atattgtgca ttaaatttag
    16741 ataaaagttt aaatgttcta gtagtatcaa aatcaacaat tgaaaacaat aatacatatc
    16801 ttgcacaagg tggaatttca actgctagaa atattgatga tatagaatcc tttgtagaag
    16861 atacaatgag ggctgggcag tataaaaata gagttgaagc agttcaagta cttgcagatg
    16921 aatctataga aaatgtaggg cagatagtag aatatggtat gcctttagat aaagaaaatg
    16981 gagagataga ttacacaaga gaaggagctc atagtgtaaa tcgaatagtt catagtaagg
    17041 ataatactgg agaagttgtt tttaaaactt tattaaaaga agctaaaact agagaaaata
    17101 taactcttat agaagatgct tatcttttag atatactaaa agatggtaat aaatgtatag
    17161 gggctagaat ttttaaatct aaaaaagaga ttcatgtttt ttcaaaaata gtagttttag
    17221 ctacaggtgg aattggtgga ctttttaaaa attctactaa tcaaagacat cttacaggtg
    17281 atggaattgc aatagcttta agaaataata taaaaataga aaatttagaa tatattcaaa
    17341 tacatcctac agctttttat gaagaaaata atgaaggtag gagaatgctt atatctgaat
    17401 cattaagggg agagggagct aagcttttaa ataaaaataa agaaagattt gttgatgaac
    17461 ttcttcctag agatgtagtt tcaaaagcta tttttgaaca aatggaaaaa gataagttac
    17521 cttatgtgta tttagatgca acacatctag atagtgagta tttaattaat agattttctt
    17581 ttatatataa tgaatgctta gctagaggaa cagatataac gaaagaatgt ataaaggtat
    17641 ctcctgcaca acattatttt atgggcggta tacatgtaga tttggattca aaaacatcta
    17701 tggaaaattt gtatgcagtt ggtgaaatta gttgtacagg tgttcatgga gccaatagat
    17761 tggcaagtaa ttcattactt gaagggcttg tattttcaaa aagggcagct aaaaatataa
    17821 actcggttat agataatgta aaagttaagt ttgtagatat accagatatg gatatagata
    17881 tagaacaggt aaaaaaagaa aataaaatat tagtcataaa agaaattgaa agaacaagtg
    17941 aggattttgg tgatgaatta tttgattatt gatagaatga tacaagatgc tttgatagaa
    18001 gatgttccaa gtgaagatat aacaacaaat tctattgtag atgaa
//
LOCUS       DAFNNB010000072        18025 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0073, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000072 DAFNNB010000000
VERSION     DAFNNB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 18025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18025
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0073"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            381..2276
                     /locus_tag="L3M60_002067"
     CDS             381..2276
                     /locus_tag="L3M60_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp8"
                     /protein_id="HBN6226731.1"
                     /translation="MLSNKKRSMAIVMAGATVMSAAAPIFADNTVTENVDKNYTVSAK
                     DSAKLIEEVRKALEVKFEDTKAGANVNDRVYDIKVDNVNLTNATQLQNKINSLTEGQS
                     LKVTIQDKGHQVLGGKVVDYKIENYKTAQEIVDAVNAYNATLAQDSDNKLTATIKSTS
                     TVEVKRAKDSTNVITLNVGDQHLDFSKVITSEDGTFEGYEKSYSDIESKELHTITVKN
                     ADLQDITAEELFDGIRLTTLGRELVNKVKNGYTLTFENEAILTQEQEDSDDKDKPEKS
                     SFDIVLSKANEKLETISVSSKNHKLVRELHKVLTDVKDGKELKVEVLSGDSRFTTAVE
                     VSKERFKDGEAEAIILVGEDAIVDGLASAPLASQKNAPILLSKKDSLPSEIESEILRV
                     LGSNLSSKKIYIVGGESKISKETEEKLSKLGVSKVERVSGEDRFETSLEIAKQLKDTF
                     KTAFVVGGNGEADAMSISARAAQFGAPIIVTGNELDANAEKLLKGKELEIVGGENSVS
                     KEVEDKLVDIDLNNKVERLAGENRKDTNAKVINKYYAGATKAYVAKDGYVGGNGQLVD
                     ALTAAPLAASSKAPIVLTTEELSKSQEEVVELRLKNATKLVQIGEGIAKNAIEKIAEK
                     INLFTKN"
     gene            2739..4142
                     /locus_tag="L3M60_002068"
     CDS             2739..4142
                     /locus_tag="L3M60_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp9"
                     /protein_id="HBN6226732.1"
                     /translation="MKNSKKILAIGLTLFLVMVNTPMVNALTSVEQIKGNDRYETAAK
                     IADKQNYNTAILVNSDNSLADGLSASGLAGALNAPILMTKQNQIPNTTMERLNKANTV
                     YIIGSESTISKNVENQLLSKKKVVQRIFGENRFDTSIKIAEKIKEIKPIDKVIIANGV
                     TGEADAISASPVAARDGVPIILTDGNSMGFDTTGLKSYALGSSEIISDELVKSTNSIR
                     LGGTDRFDTNKRVIQEFYKNSKEFYLSKGLQLTDALAASTIAKNAPVVLVENGSNKSI
                     LSGADKLTVLGGINQNVIKQCINQASPNQQGLYYNPNDRAFKERIKGKVYALTKQYRK
                     DNGVRALSVASRLEGLANDWSNLMANKKTLSHTINGKNSYSTFLKYLDWSEIKPGYIA
                     VQGENIIKYKIPDKPVYTNRDADDIGNFIFNEWKTNPEEGTNMLHKGYEIMGFGIAIT
                     GDKNLYATHEFYGRYKE"
     gene            4655..6256
                     /locus_tag="L3M60_002069"
     CDS             4655..6256
                     /locus_tag="L3M60_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp11"
                     /protein_id="HBN6226733.1"
                     /translation="MKMNKKILSLGLAVSLILVNFKSVNASSVVEKIYGKDRYETAAK
                     IADRQTYETVILVNTEKSLADGLSASGLSGVTKAPILFTQKNKIPADTNRCLKNIKKA
                     YIIGTEDTISKSVEKELNSKNIEVKRIGGEDRLKTSYLMAKEIATIKKVDKVLLTNAY
                     SGEADAMSVSSVATRDGAPIILTDGKGIPFDVKNVQSYCIGSEEIMSNSLVKNTNSVR
                     IEGTDRFETNKNVIDYFFKNADGFYVSDGYQLVDAIAAAPLTKDSPMVLVNDGSDKTV
                     LEGAKNITSVGEIDEKVVQQCTNASKSNGQPPTITIGNIEIYKGEKFDTGKLNIVAKD
                     NTGKVLPIEVDGFIDTNRVGTYILTLKATDEWGKSTEKRVEIKVLDDKSHDYNSPEFK
                     KMVSTEMYNLINSYRKEKGKETLLVSSKLEDMANAWSKYMMDKKVFAHYIDGKNAPQV
                     FSEFGMRSEENIAYIYIDSKKVQTTQDAKDLAKAIFEVWKKSPEYNANMLSDEFYSTG
                     FGLYILSDGQVHATQEFLNGNEGSL"
     gene            6474..8063
                     /locus_tag="L3M60_002070"
     CDS             6474..8063
                     /locus_tag="L3M60_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6226734.1"
                     /translation="MKVNKRILSIGLTISLIMAGAPNINALSSIEKIQGKDRYETAAK
                     IAQKQTYENVVLVNTDNTLADGLSASGLAGAVKAPILLSQRNNIPPDTEKMLKDVKKA
                     YIIGTEDSIGKSVENELKQKGIEVKRIGGDDRIETSYLIAKEIASIKSINKVFITNGY
                     TGEADAMSASSVASRDGAPIILTDGKNVPFEKKEGVQCYALGSEEIISNDLVKKTNAV
                     RLAGEDRFETNKKVIKHFYSSAKEFYLSKGYQLVDAVAGSSIAKNAPIVLVDGNSDKS
                     VLRSADKITALGGIDEKTLEQCLSASSLDASAPTITVGNLNIYQGDKFDISKLNILAK
                     DSNGNDLTPELIGNINTDKVGKYKVTIKATDIGGKTTSINVEVNVLEYKTNDMNSSEF
                     KRMVSSEMYNLVNSYRKEKGKEPLQVSKNLEGLSNSWSKYMADKGQFSHVIDGKKSVE
                     VFTGYGLRSEENIAFVPLVTKSTYTTKDAREVANVIFTVWKKSDKYNENMLNSDFVYT
                     GFGLYILSNGEVYATQEFLNK"
     gene            8276..10456
                     /gene="slpA"
                     /locus_tag="L3M60_002071"
     CDS             8276..10456
                     /gene="slpA"
                     /locus_tag="L3M60_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer protein SlpA"
                     /protein_id="HBN6226735.1"
                     /translation="MNKKNLAMAMAAVTVVGSAAPVFAADEQVKYQNTYTVVQSKYEK
                     ALKDMQKGITDKKIKSIAISYEGKPVTTITVADMDTKGKTSTKEELASALLKTTVNDK
                     LDNLGDGDYVDFDITYVGDADRLTAGDLNTFAKGIADSTEKKIPAAKGSNYGVAKTNS
                     GTGKLTTDTEAVISTSIEGKVEGNNLTISLKDAPSKVGVIGANNDTLADVTFADDAKL
                     TVSVGDPKIDLAKSFIFDTKTGKLGGIVEKENDATEHAYVRVINAKEQTIDLDASSYK
                     SAEDLAKAYAFDVNELKTLYTEIEAYQKDSNNKTDKVQIVDGKYQTILYAEGKRLTTK
                     SATQASKLADENSPLKLVIKADKLKDLKDYVEDLRNANNGYSNTVTVAGDDRIETAIE
                     LSSKYYNNSDEDNAITEDAVNNVVLVGSQAIVDGLVASPLASEKKAPLLLTSKDKLDS
                     NVKSEIKRVMNLKTTTGINNSKKVYLAGGVNSISKEVENELKDMGLKVTRLSGDDRYA
                     TSLEIADEIGLDDDKAFVVGGTGLADAMSIAPVASQLNEKGDATPIVVVDGKAKELSS
                     AAEDFLDDSQVDIIGGKNSVSKDMEDAIDDATGKSPNRVSGDDRQETNAEVLKESDYF
                     PDGAVNYFVAKDGSTKEDQLVDALAAAPVAANFGRTYNIKDNDSSGTVSPAPIILATD
                     SLSSDQNVAISKALPSGKSGDNLVQVGKGIANSVITKIKDLLDM"
     gene            10656..13001
                     /gene="secA"
                     /locus_tag="L3M60_002072"
     CDS             10656..13001
                     /gene="secA"
                     /locus_tag="L3M60_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBN6226736.1"
                     /translation="MSVLDTILDKADEQEIKKLNLIVDKIEALEEKIQNLSDDELKEM
                     TNVFRNRLNNGETLDDILMEAFAVVREVSKRKLGMRQYRVQLIGGIVIHQGKIAEMKT
                     GEGKTLVEVAPVYLNALTGKGVHVITVNDYLAERDKELMRPVYESLGMTVGVIIANQD
                     QNIRKQQYKCDITYGTNSEFGFDYLRDNMVPDLSHKVQRELNFAIVDEVDSILIDEAR
                     TPLIIAGDGDEDLKLYELANSFIKTIKEEDFEMDRKDKTIALTASGISKAESFFGITN
                     LTDIKNIELYHHINQALRGHKLMEKDVDYVISNGEVMIVDEFTGRVMDGRRYTDGLHQ
                     AIEAKEGVEINNESKTMATVTYQNFFRLYEKLSGMTGTAKTEEGEFESIYKLNVVQIP
                     TNKPVIRADLHDKVFKTEEEKYNAVVEEIIRIHKTRQPILVGTVSVEKSEKLSRMLKK
                     QGIKHQVLNAKQHDKEAEIISKAGKLDAITIATNMAGRGTDISLGAGDREEEQQVKNL
                     GGLYVIGTERHESRRIDNQLRGRSGRQGDPGTSRFFVSLEDDVIKLYGGKTIEKLMKR
                     TSSKENTAIESKALTRAIERAQKGVEGKNFEIRKNVLKYDDTINEQRKVIYNERNKVL
                     NNEDIQEDIQKMVKDIIQEAGENYLVGRKRDYYGYFKYLYSTFMPADTLLIPGVDKKS
                     VQEIVDSTYEISKRVYDLKKMMLGIDKVSELEKTVLLKVVDQYWIDHIDAMEQLRQYI
                     GLKSYAQKDPFKEYALEGYDMFEALNKNIREATVQYLYKFN"
     gene            13415..15286
                     /locus_tag="L3M60_002073"
     CDS             13415..15286
                     /locus_tag="L3M60_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp2"
                     /protein_id="HBN6226737.1"
                     /translation="MNRKNLSVIMATAMISTSVAPVFAAETTQVKKETITKKEATELV
                     SKVRDLMSQKYTGGSQVGQPIYEIKVGENLSKLKVITNIDELEKLVNALGENKELIVT
                     ITDKGHITNSANEVVAEAIEKYENSADLSAEANSITEKAKTETNGIYKVADVKASYDS
                     AKDKLVITLRDKTGTVTSKTIEVGIGDEKVDLTANPIDSTGTNLDPSAEGFRVNKINK
                     LGVAGAKNIDDVQLAEITIKNSDLNTVSPQDLYDGYRLTVKGNMVVNGTSKSISDISA
                     KDSETGKYKFTIKYTDASGKAIELTVESTNEKDLKDAKAALEGNSKVKLIAGDDRYAT
                     AVAIAKQTKYTDNVVIVNSNKLVDGLAATPLAQSKKAPILLASDNEIPKVTLDYIKDI
                     IKKSPSAKIYIVGGESAVSNTAKKQLESVTKNVERLAGYDRHTTSVAVAKAMGSFKDA
                     FVVGAKGEADAMSIAAKAAELKAPIIVNGWNDLSSDAIKLMDGKEIGIVGGSNNVSSQ
                     IENQLADIDKDRKVQRVEGETRHDTNAKVIETYYGKLDKLYIAKDGYGNNGMLVDALA
                     AGPLAAGKGPILLAKTDITDSQKSALSKKLNLGAEVTQIGNGVELTVIQKIAKILGW"
     gene            15393..16100
                     /locus_tag="L3M60_002074"
     CDS             15393..16100
                     /locus_tag="L3M60_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HBN6226738.1"
                     /translation="MVDATLFFTPHQDDETLSMGSAIIEHVEKSDTHVILCTDGSKSI
                     IRKVLDDGGTCSYHIKDVHKYSLSESEFSKDRDEEFKDSCEAMGVKESNIHIEDNRAH
                     DGELNKEKAREIILKYLEEYPDAKVKTVTPFKASGIHEDHRALGEAALELYREGKIKD
                     LRFYVEPYDYKDFKKVNPNVEVWKVLPSQEEKLLSAMNAYKRWNPESGHYAIGYHSVK
                     SHFDELATNKIQYVHAP"
     gene            16133..17965
                     /locus_tag="L3M60_002075"
     CDS             16133..17965
                     /locus_tag="L3M60_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6226739.1"
                     /translation="MKISKKIVSLLTMTFLTVTLYGNTSNASTKDTLTGSGRWETAIK
                     ISQAGWSKSDTAVLVNDNSIADALSATPFAKAKDAPILLTQSNKLDSRTKAELKRLGV
                     KNVYLIGGSIALSSEIEKQLNAENINFERISGNSRYDTSLKLAEKLNKEKSISKIVVV
                     NGEKGLADAVSVGAIAAQENMPIILSDSENGTEVADNFIDSKDIEKSYVIGGTYSISN
                     SVERNLPNATRIAGSSRSETNAKIIEEFYKDTDIKNIYVTKDGTRNKNDLIDSLAVGV
                     LAAKNSSPIVLAGNKLDTTQKDVLNTKVIDKVTQIGGLGNEDAVKSIIDMQEKTKYTI
                     ETIDELNVAIKKADANDVIIFEPEKDTSISDSFKIETNKAITVEFDGVFKKSITIDMP
                     NGDVKNFGEISDDIRIDNIKKGTLINEGSIQGIDIYSKNGCKIENTSDGDIWIITIDA
                     DAKDVYIENDGDITKISNNAPGVIIKNSGKIDLVNGNEQPAISGKKPTTNDTEYNDER
                     ARGLSVSTKPCSIPEKDRVRVTISSEPKSSRYKIYYRVVEDKPSAMYVGEKISVRSWD
                     LASKSDGSFVEKAKNGSYIEVVEINTSTNKVSRWGRSNVTDDGF"
BASE COUNT         7566 a         1898 c         3369 g         5192 t
ORIGIN      
        1 taaattctaa tttatgatga tttttgtaat atgttatgca ttttaatata tggagataga
       61 tattaattca gtgatatata tttaagtaat aagagaaagc aaaaattagt gcatagtagt
      121 atataaaagt ggagtttgaa taattttgtt agtatatgat tgtctaatat aaaatcacta
      181 taaaataagg gtttctatct ttggatataa tcataaacag ctaagtgggg gaaggggttc
      241 aagaagatat tactattgtt tgtgttagta aattatgtaa acatataaaa tatattgaaa
      301 aataatatta tatataatat tatttatatg tgagaaatac gaatataaaa aatcaaaaac
      361 aatattcgga ggacataaat atgttgagca acaaaaaaag aagtatggca atagtaatgg
      421 caggagctac agttatgagt gcagctgcac caatattcgc agataatact gtgacagaaa
      481 atgttgataa aaattacaca gtaagtgcga aagattctgc taaactaatc gaagaggtta
      541 gaaaagcatt agaagttaag tttgaagata caaaagcagg agcaaatgta aatgatagag
      601 tttacgatat aaaagtagac aatgtaaact taactaatgc aactcaatta caaaataaaa
      661 taaactcatt aacagaaggg caaagtttaa aagttactat tcaagataaa ggacatcaag
      721 ttttaggtgg aaaagtagtt gactataaaa ttgaaaacta caaaactgct caagaaatag
      781 tagatgcagt taatgcttac aatgcaactt tagcacaaga ttctgataac aagttgacag
      841 caactataaa atctacaagt acagttgaag taaaaagagc aaaagattca acaaatgtta
      901 ttacattaaa tgtaggagac caacatttag atttctcaaa agtaataaca tcagaagatg
      961 gtacttttga aggttatgaa aagagttata gtgatataga atcaaaagaa ttacatacaa
     1021 taacagttaa aaatgcagat ttacaagaca taacagcaga agaattattt gatggaatta
     1081 gattaactac attaggaaga gaactagtta ataaagttaa aaatggatat actttaacat
     1141 ttgaaaatga agcaatcctt actcaagaac aagaagatag tgatgataaa gataagccag
     1201 aaaaatcaag ctttgatata gttttaagta aagcaaatga aaagctagaa acaataagcg
     1261 tttcaagtaa aaaccataag ttagttagag agttacataa agtattgaca gatgtaaaag
     1321 acggaaaaga attaaaagta gaagttttat ctggtgattc aagatttaca acagcagtag
     1381 aagttagtaa agagagattc aaagatggag aagcagaagc aataatttta gttggagaag
     1441 atgctatagt tgatggatta gcatcagcac cacttgcatc tcaaaaaaat gcaccaatat
     1501 tattatctaa aaaagattca ttaccatcag aaatagaatc tgaaatatta agagtacttg
     1561 gaagtaatct atcttctaag aaaatatata tagtaggtgg agaatctaaa atatcaaaag
     1621 aaactgaaga aaaactttct aaattaggtg taagcaaagt tgagagagtt tctggagaag
     1681 atagatttga aacttcttta gaaatagcaa aacaattaaa agatacattt aagactgctt
     1741 ttgtagtagg tggaaatgga gaagcagatg ccatgagtat atcagctaga gctgctcaat
     1801 ttggtgctcc aataatagtt acaggtaacg aattagatgc aaatgctgaa aaactattaa
     1861 aaggaaaaga attagaaata gtaggtggag aaaattctgt atcaaaagaa gttgaagata
     1921 aattggttga tatagattta aataataaag ttgaaagact tgctggagaa aatagaaaag
     1981 atactaatgc taaagtaatt aataagtact acgcaggtgc aactaaagca tatgttgcaa
     2041 aagatggtta tgtaggtgga aacggacaat tagttgatgc acttacagca gcaccacttg
     2101 cagctagttc aaaagctcca atagtattaa ctacagaaga actttctaaa tcacaagaag
     2161 aagtagttga gctaagactt aagaatgcta ccaagttagt acaaataggt gaaggaatag
     2221 ctaaaaacgc gattgaaaaa atagcagaaa aaataaactt atttactaag aactaataaa
     2281 atataagaat gaatattaaa agtttactta gataatatgt ggttatacaa tatgtcaatg
     2341 tataagacat agtgtagata ttagaaaata tataaataat gaaatcaaaa taataagaat
     2401 aaggtatctc atttataaat tagattgaga tgccttattt tattttttga ttatagcatt
     2461 gggaaattca tactataaaa aattattata aatgatactg tttacaagga ttaaatgtct
     2521 tattaacata tattattttg aatttattac ttaattaaat aaataagtta taatgttttg
     2581 aatttatgaa gaataaaaac tttaagtata ttgtttgagt tgtatggata actatggtat
     2641 catatataaa atatggtaac aaaactgtaa cttttgattc aaaatgaaca aaaatactaa
     2701 taaaaaatgt gaaaagtata ttgggggtag agaataatat gaagaatagt aaaaagatat
     2761 tggctatagg acttacacta tttttagtaa tggtaaatac tcctatggta aatgcgttga
     2821 catcagttga gcaaataaaa gggaatgaca gatatgagac agcagcaaaa atagcagata
     2881 agcaaaacta taatacagca atactagtca attcagataa cagcttggca gatggattaa
     2941 gtgcaagtgg tttggctgga gctttaaatg cacctatttt aatgacaaaa caaaatcaga
     3001 ttccaaatac aactatggaa agattaaaca aagcaaatac tgtatatata ataggctcag
     3061 aatcaacaat aagtaagaat gttgagaatc agttactatc caaaaagaag gttgtacaaa
     3121 gaatatttgg tgaaaataga tttgatacaa gcataaaaat agctgaaaaa atcaaagaaa
     3181 ttaagccaat agataaagta attatagcaa atggagttac aggagaagca gatgcgataa
     3241 gtgcatcacc agtagcagct agagatggag tacctataat acttacagat gggaatagta
     3301 tgggatttga cacaacaggt ttaaagagtt atgcacttgg ttctagtgaa ataatcagtg
     3361 acgaattggt taagagtaca aactctatta gattaggtgg aacagataga tttgatacta
     3421 ataagagggt tatacaagaa ttttataaaa atagtaaaga attttattta agtaaagggt
     3481 tgcaattaac agacgctttg gctgcttcta caatagcaaa aaatgctcct gtggttttag
     3541 ttgaaaatgg cagtaacaag agtattttaa gtggtgcaga caaattgact gtactaggag
     3601 gcataaatca gaatgttatc aagcaatgta taaatcaagc ttcaccaaat caacaaggct
     3661 tatattacaa tccaaatgat agagcattta aagagcgaat aaagggtaaa gtatatgctc
     3721 ttacaaagca gtacagaaaa gataatggag taagagcctt atctgtagca agtagattag
     3781 aaggattagc aaatgattgg tctaatttaa tggcaaataa gaagacctta tctcatacga
     3841 taaatggaaa aaattcatat tctacttttt tgaaatacct agattggagt gagattaaac
     3901 ctggatatat tgcagttcaa ggtgaaaata taattaaata taaaattcca gataaacctg
     3961 tgtatacaaa tagagatgca gatgatatag gaaactttat ttttaatgag tggaagacaa
     4021 atccagaaga aggtactaat atgttgcata aagggtatga aataatggga tttggtatag
     4081 ctataacagg agataagaat ctatatgcta cacatgaatt ttatggaaga tataaagaat
     4141 aaataaaaac aatttgtaag ttataattac tactaatatg tcagataaat cacaaaaata
     4201 tcattaaaaa ataaaaattc atttagattg tttaaaatag gtagagaaat agcttcatat
     4261 atagtagata aaattaggag cttaattgaa aaataagtat atattgaatg aactgaaaat
     4321 aagaatgtat ttagatataa ataatatgtc taaaaataca ttcttatttt tattttcatt
     4381 gttttaataa tattgaattt aaaatttgta atagtttgag tagtattaaa tttgtttttt
     4441 tgaatgaagc agacaccttt cttttataag ttctattttg aacatattta aaatgaagta
     4501 aaaaataaat ttttaaattt aataaaataa aatattaata tatagactgg atatattaat
     4561 atcatatata ctctataata tgtggaaaat aaagaaaaaa taaatggaaa ataatcgtaa
     4621 tgtaaatata aaatataaaa atcatcaggg ggatatgaaa atgaacaaaa aaatattatc
     4681 attaggtcta gcagtatcat taatcttagt aaacttcaaa agtgtaaatg catcatcagt
     4741 agtagaaaaa atatatggta aagatagata tgaaacagca gcaaagatag ctgatagaca
     4801 gacttatgaa acagtaattt tagtaaatac agaaaaatca cttgcagatg gattgagtgc
     4861 aagtggatta tcaggtgtaa caaaagcgcc aatactattt acacaaaaaa ataagatacc
     4921 agcagacaca aatagatgtt taaaaaatat caaaaaagca tacataatag gtacagaaga
     4981 tactataagt aaatcagtag aaaaggaact aaattctaaa aatatagaag tcaaaagaat
     5041 tggtggagaa gataggctaa aaacaagcta tttaatggct aaagaaatag caactataaa
     5101 gaaagtggat aaagtactat taactaatgc atatagtgga gaagcagacg caatgagtgt
     5161 atcatcagta gctactagag atggagctcc aatcatactt acagatggaa agggtatccc
     5221 ttttgatgtc aaaaatgttc agtcatattg tataggttca gaggagataa tgagtaactc
     5281 attagttaaa aatacaaact cggtaagaat agaaggaact gacagatttg aaaccaataa
     5341 aaatgtaata gactactttt ttaagaatgc agatggattc tatgtatcag atggatacca
     5401 attagtagat gcaatagcag ctgcaccact tactaaagac tctccaatgg tgctagtaaa
     5461 tgatggaagt gataagactg tattagaagg agctaagaat ataacttctg taggtgaaat
     5521 agatgagaag gtagtacaac aatgtacaaa tgcatcaaag tcaaatggac aacctccaac
     5581 aattacaata ggaaatatag agatatataa gggtgaaaag tttgacactg gcaaattaaa
     5641 tatagtggct aaagataata ctggaaaggt attaccaata gaagttgatg ggtttataga
     5701 tacaaatagg gtaggtacat acatattgac attaaaagct acagatgaat ggggaaaaag
     5761 tacagaaaaa agagtagaaa taaaagtgtt ggatgataaa tctcatgatt acaacagccc
     5821 agaatttaaa aaaatggtat ctacagaaat gtataactta atcaattcct atagaaaaga
     5881 aaaaggcaaa gagactttgt tagtgtctag taagttagaa gacatggcaa atgcatggtc
     5941 taagtatatg atggataaaa aagtatttgc acattatata gatggtaaaa atgctccaca
     6001 agtattttct gagtttggaa tgagaagtga ggaaaatata gcatatattt atatagattc
     6061 taagaaggtt caaacaactc aagatgcaaa agatttggca aaggctatat ttgaggtgtg
     6121 gaagaagtct ccagagtata atgcaaatat gcttagtgat gagttctata gtactggatt
     6181 tgggctttat atattatctg atggtcaagt gcatgctact caagagtttt taaatggcaa
     6241 tgaaggtagc ttgtaaaaaa ttccataaat gttgtttttt tgttaattta ctgatattat
     6301 aaaatcaaaa tcacatctaa agtacttttt gtaatctgtt ttgttattgc tttataggtt
     6361 atggtatttt agatgttttt tgttttagta gtttgtaaat tttgagggaa tcactactgg
     6421 ggatgtaatt tatgattagc tgaattatat aaaaaattag taggaggagt tcgatgaagg
     6481 taaataagag aatattatcg ataggattga caatatcatt aataatggca ggagcaccaa
     6541 atatcaatgc actttcaagt atagaaaaga tacaagggaa agatagatat gagacggctg
     6601 ccaaaattgc acagaagcaa acgtatgaaa atgtagtatt agtaaataca gataatacac
     6661 tagctgacgg actaagtgca agtggattag caggagcagt taaagcacct atattattat
     6721 cacaaagaaa taatatacca ccagacacag aaaaaatgtt aaaagatgta aagaaggctt
     6781 atataatagg aactgaagat tcaataggaa aatcagttga aaatgaatta aagcaaaaag
     6841 ggatagaagt taaaagaata ggtggtgatg atagaataga aacaagttat cttatagcaa
     6901 aagagatagc ttcaataaag tctataaata aggtttttat aactaatggt tatacaggtg
     6961 aagcagatgc tatgagtgcg tcatcagtag catcaagaga tggtgcacct ataattttaa
     7021 ctgatggtaa aaatgttcca tttgagaaaa aagaaggtgt gcaatgttat gctctgggtt
     7081 cagaggagat aataagtaat gatttagtta aaaaaactaa tgcagttaga ttagctggag
     7141 aagatagatt tgagactaat aaaaaagtta taaaacattt ttatagttca gcaaaagaat
     7201 tttatctttc taaaggttac caattagtag atgcagttgc tggttcttct atagctaaaa
     7261 atgcacctat tgtattggtt gatggaaata gtgataagtc tgtgcttaga agtgctgata
     7321 aaatcacagc attaggagga atagatgaaa agacactaga acaatgttta agtgcatcat
     7381 cactggatgc aagtgcacca acaataactg ttggaaattt aaatatttat caaggggata
     7441 agtttgatat aagtaaactt aatatattag caaaggatag caatggtaac gatttgacac
     7501 cagagcttat aggaaatatt aatactgata aggttggtaa atacaaagtt actataaaag
     7561 ctactgatat tggtggaaag actacatcaa taaatgtaga agtaaatgta cttgaatata
     7621 agactaatga tatgaatagt agtgagttta aaagaatggt ttctagtgaa atgtataatt
     7681 tggtaaattc atatagaaaa gaaaaaggta aagaaccatt acaagtttct aaaaacttag
     7741 aagggctttc taattcctgg tctaagtaca tggcagataa aggccagttt tcacatgtaa
     7801 tagatggtaa gaaatctgtt gaagtattta ctggatatgg gttgagaagt gaagagaata
     7861 tagcttttgt accacttgtt acaaaaagta cttatacgac aaaggatgct agagaagtag
     7921 caaatgtaat atttacagtt tggaaaaagt cggataaata taatgaaaat atgctaaata
     7981 gtgattttgt atatactggg tttggactgt atatattgtc taatggagaa gtgtatgcta
     8041 ctcaagaatt tttaaataag taaaattaaa ttttttaagt tattaaatat tgaaatttaa
     8101 gggtataaaa actcatatac ttgggaaaaa taataatttc aaaaaaatgc cttgcaaaaa
     8161 gaattaaaat ggattattat agagatgtga gaaatattag gaatatatgg atgattattc
     8221 tatgtacata ataaagagat gtaattttaa tataatgttg ggaggaattt aagaaatgaa
     8281 taagaaaaat ttagcaatgg ctatggctgc tgttacagta gtaggttcgg ctgcaccagt
     8341 atttgcagca gatgaacaag ttaaatacca aaacacatat actgtagtgc aaagtaaata
     8401 tgaaaaagct ttaaaagata tgcaaaaggg gattacagat aaaaagataa aatcaatagc
     8461 aatatcttat gaaggtaagc cagttactac tattactgtt gcagatatgg atacaaaagg
     8521 aaaaacttca acaaaagaag aattagcaag tgctttatta aagactactg ttaatgataa
     8581 gttagataat ttgggtgatg gagattatgt agattttgat ataacttatg ttggtgatgc
     8641 tgatagactt actgcagggg atcttaatac ttttgcaaaa ggtatagcag atagtactga
     8701 aaagaaaatt cctgctgcta aaggttctaa ctatggagtt gctaaaacaa attctggaac
     8761 tggaaaactt actacagata cagaggcagt tatatctact tcaatagaag gtaaagttga
     8821 gggaaataat cttacaataa gtcttaagga tgctccaagt aaagttggtg taataggtgc
     8881 taataatgat acacttgcag atgttacatt tgcagatgat gcaaaattaa ctgtatcagt
     8941 tggagatcca aaaatagatt tagcaaaatc atttattttt gatactaaaa ctggtaaatt
     9001 aggtggaata gtagaaaaag aaaatgatgc aactgaacat gcatatgtaa gagtaataaa
     9061 tgctaaagaa caaacaatag atttagatgc tagttcatat aaatcagctg aagatttagc
     9121 taaagcttat gcatttgatg taaatgagct taagactctt tacactgaaa tagaagcata
     9181 tcaaaaagat agtaataata aaactgataa agttcaaata gttgatggta aatatcaaac
     9241 aatactttat gctgaaggaa agagattaac tactaaatca gcaactcaag cttctaaatt
     9301 agcagatgaa aactcaccac ttaagttagt tataaaagca gataaattaa aagatttaaa
     9361 ggattatgtt gaagatttaa gaaatgctaa taatggttac tcaaatactg ttactgtagc
     9421 aggtgatgat agaatagaaa ctgcaataga attaagtagt aaatactata ataattcaga
     9481 tgaagacaat gctataacag aagatgctgt taataatgtt gtattagttg gatctcaagc
     9541 aatagttgat ggacttgttg catcaccttt agcttcagag aagaaagctc ctttattatt
     9601 aacttcaaaa gataaattag attcaaatgt aaaatctgaa attaaaagag ttatgaattt
     9661 aaaaactaca acaggaataa acaattctaa gaaagtttac ctagctggtg gagttaattc
     9721 tatatctaaa gaagttgaaa atgaattaaa agatatggga cttaaagtaa caagattatc
     9781 aggagatgat agatatgcta cttcattaga aatagctgat gaaataggtt tagatgatga
     9841 taaagcattc gtagtaggtg gaacaggact tgcagatgct atgagtatag ctccagttgc
     9901 ttctcaatta aatgaaaaag gtgatgctac accaatagtt gtagttgatg gaaaagctaa
     9961 agaattaagc tcagcagctg aagatttctt agatgattca caagttgata taataggtgg
    10021 taaaaatagc gtttctaaag acatggaaga tgctatagat gatgctacag gaaaatctcc
    10081 aaatagagtt agtggagatg atagacaaga aactaatgca gaagtattaa aagaatctga
    10141 ttatttccca gatggtgcag taaattactt tgttgctaaa gatggatcta caaaagaaga
    10201 ccaattagtt gatgcattag cagcagcacc agtagcagcc aactttggaa gaacttataa
    10261 tataaaagat aatgatagtt ctggaacagt ttctccagca ccaatcatat tagcaactga
    10321 ttcattatct tcagatcaaa atgttgcaat aagtaaagca ttaccttctg gaaaatcagg
    10381 agataattta gtacaagttg gtaagggtat agcaaactca gtaatcacta agataaaaga
    10441 cttattagat atgtagtata aatttaaaat acaaagtaaa aaagattcct attttaggaa
    10501 tcttttttac tttgtatttt aaaaaatacc taactgatta agagatgtaa ttcattcaag
    10561 tatgattatt atactataaa taaaaaagtt gtctactatt ccacattttt aaggtataat
    10621 aatagttgta tgataaaaaa ggaggtaaga aacacatgtc agttttagat acaatacttg
    10681 ataaagcaga tgaacaagaa attaagaagt tgaatttaat agtagataaa atagaggctt
    10741 tggaagaaaa aatacaaaac ttatctgatg atgaattgaa agaaatgact aatgtattta
    10801 ggaatagatt aaataatgga gaaactttag atgacatatt aatggaagct tttgcagttg
    10861 taagagaagt atcaaaaaga aaattaggaa tgcgtcaata tagagtacag ttaattggtg
    10921 ggatagtaat acatcaaggt aaaatcgctg agatgaaaac aggtgaaggt aaaacgttag
    10981 ttgaagtagc accagtatat ctaaatgctc ttacaggtaa gggtgtacat gtaatcacag
    11041 taaatgatta cctggcagaa cgtgataaag aacttatgag accagtttat gaatctcttg
    11101 gtatgacagt aggagtaatt atagctaacc aagaccaaaa tataagaaaa caacagtata
    11161 aatgtgatat aacttatggt acaaatagtg aatttggatt tgattattta agagataata
    11221 tggttccaga tttatctcat aaggtacaaa gagaactaaa ctttgccata gtagatgagg
    11281 tagactcaat attaatagat gaagctagaa ctccgcttat tattgcagga gatggagatg
    11341 aagatttaaa actttatgaa ttggcaaata gctttataaa aactattaaa gaagaagact
    11401 ttgagatgga tagaaaagat aaaactatag cattaacagc aagtggtata agcaaagctg
    11461 agtcattttt tggtataaca aaccttactg atataaagaa tatagaatta tatcatcaca
    11521 taaatcaagc tttaagaggt cataagctta tggaaaaaga tgttgactat gttatctcaa
    11581 atggagaagt aatgatagtt gacgaattta caggaagagt aatggatggt agaagatata
    11641 cagatggact tcaccaagct atagaagcaa aagaaggtgt tgagataaat aatgaatcta
    11701 aaactatggc tactgtgact tatcaaaatt tcttcagact atatgaaaaa ctttctggta
    11761 tgactggtac tgcaaagaca gaagaagggg aatttgagtc aatctataaa ctaaatgttg
    11821 ttcaaatacc aactaataaa ccagtgatta gagctgattt acatgataag gtatttaaga
    11881 cagaagaaga aaagtataat gctgttgtag aagaaataat aaggatacat aagactagac
    11941 aaccaatact tgtaggaaca gtttctgttg aaaaatctga aaaattatct agaatgctta
    12001 aaaaacaagg tattaagcat caagtcttaa atgcaaaaca acatgataaa gaggcggaga
    12061 taatttctaa agctggtaaa ttagatgcta taacgattgc tacaaatatg gcaggtagag
    12121 gaacggatat ttctctaggt gcaggagata gagaagaaga acaacaagtt aaaaatttag
    12181 gtgggcttta tgttatagga acagaaagac acgaatcaag aagaattgat aatcagctta
    12241 gaggacgttc tggtcgtcaa ggagacccag gtacatcaag attctttgta agtcttgaag
    12301 atgatgtaat aaagctttat ggtggaaaaa ctatagagaa acttatgaag agaacaagtt
    12361 caaaggaaaa tactgctatt gaaagtaaag cacttacaag agctatagaa agagctcaaa
    12421 aaggtgtaga aggtaaaaat tttgaaataa gaaaaaatgt ccttaaatat gatgatacta
    12481 ttaatgaaca aagaaaagtt atatataatg aaagaaataa agttttaaat aatgaagaca
    12541 ttcaagaaga tattcaaaaa atggttaaag atatcataca agaagcagga gaaaattatt
    12601 tagttggaag aaaaagagat tattatggat attttaaata tctatatagt acatttatgc
    12661 cagcagatac actattaata cctggagtag ataaaaaaag tgttcaagaa atagttgata
    12721 gtacttatga aatttcaaaa agagtttatg atttaaaaaa gatgatgctt ggtattgata
    12781 aggtttcaga gttggaaaag acagtacttt taaaagtagt tgaccaatac tggatagacc
    12841 atatagatgc tatggaacag ttaagacagt atataggtct taaatcttat gctcaaaaag
    12901 acccgtttaa ggaatatgct ttagaaggat atgacatgtt tgaagcttta aataaaaata
    12961 taagggaagc aacagtgcaa tacttatata aatttaacta aaaaagtgca tataattttt
    13021 attaaatgaa aaataaagta ttttgattct ttatattgta attacaatta aataattaaa
    13081 tatataaatg aaaaagaagc tgaaaatcag cttctttttc tgtgtgagaa atacggaaaa
    13141 aagagatgct tgaaatttct ttcttccatc taatattata ctataaagaa gatgaaaagt
    13201 ctattagtta taaactggat tattaaaaaa aattacagtt ttgttacttt tttgtataca
    13261 taaatattaa aaatttaaag tattagaaaa aatttatatc ttttggttaa ttattacaat
    13321 aagtctcatt tattgaaata atatcaaata tatattataa ttggtaaata aggaaaaaat
    13381 gataaaaaat tgaatttttt agggggaaaa taccatgaat agaaaaaatc tttctgtaat
    13441 tatggctact gcaatgataa gtacatcagt agctccagtt tttgctgcag aaactacaca
    13501 ggtaaaaaaa gaaacaataa ctaagaaaga agctacagaa ctagtttcaa aagttagaga
    13561 tttaatgtct caaaagtata caggaggttc tcaagttgga caaccaatat atgaaataaa
    13621 agttggtgag aatttatcaa aattaaaagt aataactaat atagacgaat tagagaaatt
    13681 agtaaatgct ttgggagaaa ataaagaact tattgtaact ataacagata aagggcatat
    13741 aacaaatagt gcaaatgaag tagttgcaga agcaattgaa aaatatgaaa attcagcaga
    13801 cctttccgct gaagctaatt ctataacaga aaaagctaaa actgaaacta atggaattta
    13861 taaagttgca gatgtaaaag cttcatatga tagtgctaaa gataagttag ttataacttt
    13921 aagagataaa acaggaacag taacttctaa aactatagag gtaggtattg gtgatgaaaa
    13981 agttgattta acagcaaatc caattgattc aactggaaca aacttagacc cttctgcaga
    14041 aggatttaga gtaaataaaa tcaataaact aggtgtagca ggagctaaaa atattgatga
    14101 tgttcaatta gctgaaataa ctataaaaaa tagtgaccta aatacagttt caccacaaga
    14161 tttatatgat ggatatagat taactgttaa aggtaatatg gtagtaaatg gtacatcaaa
    14221 gtcaattagt gatatttcag caaaagattc agaaacagga aaatataaat ttactattaa
    14281 gtatactgat gcatctggaa aagcaataga gcttactgta gaaagcacta atgaaaaaga
    14341 tttaaaagat gccaaggctg cattagaagg taattcaaag gttaaattga tagctggaga
    14401 tgatagatat gcaactgcag tggctatagc aaaacaaaca aaatatactg acaatgtagt
    14461 tatagttaat tcaaataaac tagttgacgg attagcagct acaccactcg ctcaatctaa
    14521 aaaagcacct atattgttag catctgataa tgaaatacca aaagtaactt tagattatat
    14581 aaaagatata attaagaaaa gcccatcagc taaaatatat atagtaggtg gagagtcagc
    14641 agtatcaaat acagctaaaa agcaattaga atcagtaact aagaatgttg aaagactagc
    14701 tggatatgat agacatacga cttctgtagc agtagcaaaa gctatggggt cttttaaaga
    14761 tgcatttgta gtaggtgcaa aaggggaggc tgatgctatg agtatagctg ctaaagctgc
    14821 tgaacttaag gctcctataa tagtaaatgg ctggaatgat ctttcatcag atgctatcaa
    14881 actgatggat ggaaaagaga ttggcatagt tggtggttct aacaatgtat ctagtcaaat
    14941 tgaaaatcaa cttgctgata ttgataaaga tagaaaagtt caaagagttg aaggagaaac
    15001 aagacacgat accaatgcta aagttataga aacatattat ggaaaattag ataaactata
    15061 tatagcaaaa gatggatatg gaaataatgg tatgctagta gatgcattgg cagcaggacc
    15121 tctagcagca ggtaaaggtc caatacttct agctaaaact gatataacag actcacaaaa
    15181 gagtgcactt agtaaaaaat taaatcttgg tgcagaagta actcaaatag gtaatggagt
    15241 tgaattgaca gtaatacaaa agatagctaa aatactaggt tggtaataac ttatactgtg
    15301 gagaagattc cttaataaat acaggaatct ttttctataa ctatgctatt tattgtaaat
    15361 tataacattt tatgaagtag gaggatgtag tagtggtaga tgcaacttta ttttttacac
    15421 cacatcaaga tgatgaaact ttaagtatgg gaagtgctat aattgaacat gttgaaaaaa
    15481 gtgatacaca tgtcatatta tgtacagatg gtagtaaatc tataataaga aaagttttgg
    15541 atgatggagg cacatgttct tatcatatta aagatgtaca taaatattct ttatcagaaa
    15601 gtgaattttc aaaagataga gatgaagaat ttaaggatag ttgtgaagca atgggtgtaa
    15661 aagaaagtaa tatacatata gaagataata gagcccatga tggcgagtta aataaagaaa
    15721 aggcaaggga aataatactt aagtacttag aagaatatcc agatgcaaaa gtaaaaacgg
    15781 ttactccgtt taaagctagt ggaatacatg aagaccatag agcattagga gaagctgcac
    15841 tagagttata tagagaagga aaaataaaag atttaagatt ttatgtagaa ccatatgact
    15901 ataaggattt taaaaaagtt aatccaaatg tagaggtatg gaaggtatta cctagtcaag
    15961 aagagaagtt attaagtgca atgaatgcat ataagagatg gaatcctgaa agtggacatt
    16021 atgctatagg ataccattca gtaaaatctc attttgatga gttagctaca aataaaatac
    16081 agtatgtaca tgctccataa aatattacga aagaattagg aggtaagaat taatgaaaat
    16141 atcaaaaaag atagtgtctt tgttaactat gacattttta actgttacat tatatggaaa
    16201 tacatctaat gcatctacaa aagacacatt aacaggttct ggaagatggg aaacagcaat
    16261 aaaaataagt caagctgggt ggtcaaagtc tgatacagct gtattagtaa atgacaattc
    16321 catagcagat gctttatcag ctactccatt tgcaaaagca aaagatgcac caatattatt
    16381 aactcaaagt aataaattag atagtagaac caaagcagaa ttaaaaagac ttggtgtaaa
    16441 aaatgtatat ttaataggag gttcaattgc attaagttca gagattgaaa agcaattaaa
    16501 tgcggaaaat ataaattttg aaagaatatc tggaaatagt agatatgata cttctttaaa
    16561 actagctgaa aagttaaata aggaaaaatc tatctctaaa atagtagtag taaatggaga
    16621 aaagggactt gctgatgcag taagtgttgg agctatagct gctcaagaaa atatgccaat
    16681 aatactttct gattcagaga atggaactga agtagctgat aattttatag atagtaaaga
    16741 tatagaaaaa tcgtatgtaa taggtggtac atattctatt tctaattctg tagaaagaaa
    16801 tttaccaaat gcaacaagaa tagcaggcag tagtagaagt gaaacaaatg caaagattat
    16861 agaagaattt tataaagata ctgacataaa aaatatttat gttacaaaag atggtacaag
    16921 aaataagaat gatttaatag attctttagc agtgggtgta ttagcagcta aaaatagttc
    16981 tccaatagta ttagcaggaa ataagcttga tactactcaa aaagatgtgt taaatactaa
    17041 ggttatagat aaagttactc aaattggtgg tttagggaat gaagatgctg taaaaagcat
    17101 aatagatatg caagagaaga ctaaatatac tatagaaact attgatgagt taaatgttgc
    17161 tataaaaaag gcagatgcaa atgatgtaat aatatttgaa ccagagaaag atactagtat
    17221 aagtgattca ttcaagatag aaacaaataa agctataact gtagaatttg atggagtatt
    17281 taaaaaatct ataactatag acatgcctaa tggagatgta aagaattttg gagaaatttc
    17341 agatgacata agaatagata atataaagaa aggtactttg attaatgaag gtagtataca
    17401 aggtatagat atttattcta agaatgggtg taagatagaa aatacaagtg atggagatat
    17461 atggattata actatagatg ctgatgctaa agatgtatat atagaaaatg atggagacat
    17521 aacaaaaata tcaaataatg ctccaggtgt tataataaaa aattcaggaa agattgattt
    17581 agtaaatggt aatgagcaac ctgcaattag tggaaagaaa ccaacaacta atgatactga
    17641 atataatgat gaaagagcgc gtggattgtc tgttagtaca aagccttgct caatacctga
    17701 aaaagatcgt gtaagagtga cgatatctag tgaacctaag agttcaagat ataaaattta
    17761 ttatagagtt gtagaagata aaccttctgc tatgtatgta ggagaaaaaa ttagtgtaag
    17821 aagttgggac ttagcatcaa aatcagatgg atcttttgta gaaaaagcta aaaatggatc
    17881 atatatagaa gttgttgaaa taaatacctc aactaataaa gtttctagat ggggaagatc
    17941 aaatgtaact gatgatggat tttaaatcac cttaaataaa gttaaaaaag tagattacta
    18001 tttctagtag tctacttttt atttt
//
LOCUS       DAFNNB010000073        17505 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0074, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000073 DAFNNB010000000
VERSION     DAFNNB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17505
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0074"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(494..871)
                     /locus_tag="L3M60_002076"
     CDS             complement(494..871)
                     /locus_tag="L3M60_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6226740.1"
                     /translation="MTISKIPQAELKVMKFIWEKNDTVTSKETIEAMEQKYGWKQTTT
                     LTLLSRLVKKHFLSAEKIDRYTHYTIVVGHKEYLSVETKDFLSNIHDNSLQSLISALH
                     DDEVVDKDMLDLFENHFKNLEEE"
     gene            complement(1058..2027)
                     /locus_tag="L3M60_002077"
                     /pseudo
     CDS             complement(1058..2027)
                     /locus_tag="L3M60_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2061..2258)
                     /locus_tag="L3M60_002078"
     CDS             complement(2061..2258)
                     /locus_tag="L3M60_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBN6226741.1"
                     /translation="MELWKRNLFVCWIGMFFSSIGMSQIAPILPLYIKQLGVTDVSLI
                     QQYSGIIFGCTFVVAAFFSPI"
     gene            2744..3346
                     /locus_tag="L3M60_002079"
     CDS             2744..3346
                     /locus_tag="L3M60_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6226742.1"
                     /translation="MEVCILMGSPKKKGNTATILKPFIEELENYNSNIEVIWLYDCKI
                     EPCIACKKCQDNLFDFGCYCKDDVQKIFKKVLMCDLIVFATPIYSWYCTSPMKALLDR
                     LVYGMNKYYGNEKGPALWSGKPVALITTCGYKPENGADIWENGMRRYCKHSQLKYIGM
                     LVERDLGKPNFMNKEKEEHSRQFAKQIYSILDNSIKVDNY"
     gene            complement(3622..4593)
                     /locus_tag="L3M60_002080"
     CDS             complement(3622..4593)
                     /locus_tag="L3M60_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HBN6226743.1"
                     /translation="MKKVISIGEALIDFIPNQNGCKLKNVSGFRRVAGGAPANVSAVV
                     AKLGGKSSFISKLGKDAFGDYIIDVLNEVNVNTDYVLRTSKANTGLAFVSLKEDGNRD
                     FSFYRNPSADMLLEADEVKKEWFNNCHSLHFCSVDLIDSPMKLAHKKAIEYALESNSI
                     ISFDPNIRLPLWDSDQSCKNAISEFLPFAHIVKISDEELEFITGENDIEKSLHKLFVG
                     NVELVLYTKGKDGVDAYTKKVKGMCKGIKVNAIDTTGAGDSYIGSFLYTLLCKQITLN
                     DIKEIKQETLNEYLEFSNYYAARSTTIKGAISSYATKEEISEFIKNL"
     gene            complement(4619..6100)
                     /locus_tag="L3M60_002081"
     CDS             complement(4619..6100)
                     /locus_tag="L3M60_002081"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBN6226744.1"
                     /translation="MYKEPKYRTILESTKEELNDLRNISLNDKYKPIFHIHPQHGLLN
                     DPNGLAYYNGKYHVFYQWYPYDVTHGMKHWAYVSSDDFVNWNREDVALIPIESYESHG
                     AYSGNSIEVDGKLHMYYTGNIKYSAEDRYAYQNLAIMDKDGKITKYENNPIVSEIPKG
                     YTGHVRDPKVFKRKDKYFMLLGAQTSNKKGAIIVYESKNSIDWNFKGELNVKNIDEDF
                     GYMWECPDYINIDGKDILIFSPQGLEPKGFNYQNIYNVVYAIGNMDLDNLTFEIDIMK
                     EMEKGFDFYAPQTFIKDSQTILFAWAGMGEVLYPTDKNKWAHCLTVPRKLNIKNNKLL
                     QMPVDELVKLRYDEISGQNTIKKNINIIENDENVYELNINIKNIDSNKFGLELFSSQD
                     EGVKLEFNKVENTVTLDRSNFKKVFSVEYGTNRKEYINIDENTNIKVLADRSILEIFI
                     NNGEAVFTSRIFAKENSNQIKIYSDKIVCYKYTKFKLKQGIEL"
     gene            complement(6192..7622)
                     /locus_tag="L3M60_002082"
     CDS             complement(6192..7622)
                     /locus_tag="L3M60_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBN6226745.1"
                     /translation="MSAKTIDYNKVSKQLLEYIGNEQNIKGVAHCATRLRIVLDSNNK
                     ANIKAIEELEGVKGVFIASNQLQVVFGAGIVNNVYKEFSKLSGFENMSLSDVKSESTQ
                     KQNKFQQAIKSLSDVFVQIIPGLLAAALLMGITGLLGQRGIFGELSIIEMYPQLAGLN
                     RFISIASTGIFTILPLLVVYSSTMRFGGSPVLGLILGAIMLHPELTNAYSVGNGSVVP
                     EIIDLFGLKVELVGFQGGIIVALMMGFVVAKLDKFFNSKIHDMLKLFLAPICTVIVAS
                     FLLFVIVGPLGRGLANIVTSSLLWITENLGIFGYMIFAGVQQVIVITGLHHVIGAVEA
                     QLIADTGTNFIMPLMSVALIGQGGAILGYLVLNWKDIKARQIGISSFASVLFGISEPA
                     IFGVTLKYKYPLIAGCIGGAIGGAYIYMTKVTALAFGATAVPGLAIVSTQGGGHVQYI
                     LAHLVTIVVAMITTILLGKLQKKSVN"
     gene            complement(7791..8777)
                     /locus_tag="L3M60_002083"
     CDS             complement(7791..8777)
                     /locus_tag="L3M60_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBN6226746.1"
                     /translation="MKKITINDIAGLAGVSKSTVSRYLNNKDISDSTKEKIKTIIDEY
                     GYEPNAFAQSLRAKKTYFIGIIAPCLDSFVKSKIMMAIDEELKELKYTSLIINTSRKI
                     RSEIDSISKLARLKVDGIILVGTEITKEHKNEIEKLDIPIVVVGQKVDGINSIVNDDY
                     GAGYKMGQYIATKGYKNIVYLGVDESDISVGSNRKNGVLNGLKDKGYDAKVFYTDFDQ
                     ETSIQKSGEMLENENPDMIICATDTIAIATMKEINKRGKNIPQDISVAGFGGYDVLSI
                     ISPKLTTIKFENESAGKVAANTIVNLIQERKEPLLKEIKFELIEGESTINKN"
     gene            complement(9260..10153)
                     /locus_tag="L3M60_002084"
     CDS             complement(9260..10153)
                     /locus_tag="L3M60_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6226747.1"
                     /translation="MAKLTLEHIKGNTYYIQLPTIVGVYVDGKDAILIDSGNNKDTAR
                     QVLRLLEEHNLIPKLIINTHSNADHIGGNAYLKNQTKCKIATTKIEGYFTENPILESA
                     FLYGGYPSKALKNKFLLAKESEVDYIIPSNGKIVDTELEAISLPGHYFEMIGVKTPDN
                     VLFVADSLTPENIITKYHFFFLLDIDSQFKTLEKLRMLEADFFVPSHSVKTTDIKNLI
                     DINKKKMEEIIDNIKKVCCEPVMIDKVIEKMCDLYNVKLDANQYVLVGSTIRSYITYL
                     YENNMVEYIFDGGKMMIKVLQ"
     gene            10302..11162
                     /locus_tag="L3M60_002085"
     CDS             10302..11162
                     /locus_tag="L3M60_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBN6226748.1"
                     /translation="MLLKKDRITFSEILILIVGCILMAISLNLFFNPHAIAAGGITGL
                     GVVLNSLFGVELWIVNLLLNVPLFIFAYKILSKKDCFKTVLGIIFLTIALKLTANMAT
                     LDITNDMYLAIISGSILMGVGQGLIFRINGSTGGTDLMALLLNKYFPTFSIPVLMGIV
                     DCVVVVLSGIVNRQVEIALYSTVALYILVKVSDLLIEGFNYSKSFTIISDLSKDISKK
                     IMEDLDRGATILKGEGAYTGENKNVLLVVVEKKEVVELKKLVKNVDPDAFIIITDIHE
                     ALGNGFKKIE"
     gene            11431..11997
                     /locus_tag="L3M60_002086"
     CDS             11431..11997
                     /locus_tag="L3M60_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN6226749.1"
                     /translation="MSVSLQKGQRVSLVKDNNPVKNLIVGLGWDMNKLGKKNYDLDAF
                     AIALTNQDKMNARGDLVYFGNLKHPSKAIIHTGDNLTGKGEGDDEQIIVNLENIPEYV
                     HKIVFGVSIYKAKKRDQDFGQMNNSFIRLIDSNSKQELFKYNLQEDFSGKVTVLAGEI
                     YRRNEEWKFNALGIGQNEELRELINTYK"
     gene            12036..12617
                     /locus_tag="L3M60_002087"
     CDS             12036..12617
                     /locus_tag="L3M60_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN6226750.1"
                     /translation="MGITLAKGQKVSLTKSNPGLKKVIVGLGWDINKYDGGFDFDLDA
                     SAFLTGTNGKVTNDGDFIFYNNLKHVSGAVEYMGDNRTGVGDGDDEQINVDLSKIPQN
                     IAKISFSVTINEAITRRQNFGQVENSYIRIYNEETNEELIKYELGEDFSIETAIVVAE
                     LYRHNGEWKFNALGSGFEDGLAGLCKNFGVNIG"
     gene            complement(12679..13596)
                     /locus_tag="L3M60_002088"
     CDS             complement(12679..13596)
                     /locus_tag="L3M60_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6226751.1"
                     /translation="MTIRSLEIFVKVAECGKMSEVARNMYITQSSVSQAISEIEKEYG
                     VKLFDRISKKLYLTEAGKKLLGYGRHLLAVNEEMNDCMKHCAKNIRIRVGATVTVGTC
                     LISPIMLELYKVNPLIEPEVFVEDTRLIAKKLLNSELDIAIVEGKIKHPDIVTKSIIN
                     DNLVLICSHKHEFYKRDSIKVSELSNQPLIMRELGSGTRAQLEKQLKELKIPMNIRWS
                     CYNSEAILRAVVDNFGIAVISELLIEDYLKKHLLWACDIEGINLHRTFDIAYHRSKFF
                     TENISAFFDISVKYGKKQARKKSRSVNSL"
     gene            13726..15354
                     /locus_tag="L3M60_002089"
     CDS             13726..15354
                     /locus_tag="L3M60_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN6226752.1"
                     /translation="MKVLIIGGVAAGTKTAAKIKREMGDNCSVTILNKGDDISYAGCG
                     LPYYVGKVIEDKSSLIVNTPESFSKLTGAEVRCGVEVTSIDRNAKQVTLKTLSTGEKE
                     NLSYDKLVIATGADPIKPPIPGIDLPGVFFMRTPNDAIKLRDSIENVKRAVVIGGGFI
                     GLEIAENLSAMGIRVSVIDMAEHVMPGFDTDFAEYVENHMADKGIMIFTGDQVVGIEG
                     ENKVEKLRTKNRAIKTDLVVMSVGIRPNTGFLKDTGLEFAPNKTLIADEYMLTNDKDI
                     YVVGDCAFVKNSLTNKPTWSPMGSSANHEGRICAQNISGKSKTYNGVLGTTVVKLPEL
                     NAGKTGLSKEIAEKEGYNVCSVTIATDDKAHYYPGASNFIIRMVAEKNTRKLLGVQVM
                     GPGAVDKIVDIAATAITLGADLYSLESMDLAYAPPFSTAIHPFVVAVNVLQNKLNGEL
                     DSVLLDEIEDFDSWTKLDVSKAPSIPSLRYIPVGEINGEIEGLAKDEKILLICAKGKN
                     SYMAQNRLKRFGYTNTKVLEGGILFYPNLKVMEE"
     gene            15357..16304
                     /locus_tag="L3M60_002090"
     CDS             15357..16304
                     /locus_tag="L3M60_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBN6226753.1"
                     /translation="MSNVTAEQKKEVKGRGFLPSRDGEHFAARIITVNGVINASQAKK
                     IAEAAEKFGNGQLAFTTRLTIELPGVKFEDIEALSEFIASENLITGGTGSRVRPVVAC
                     KGTVCVHGLADTQALAAEIHQEFYKNWYDVKLPHKFKIGVGGCPNNCIKPDLNDLGIV
                     GQRVPDYDSELCVGCKKCAVVEACPVKAAKLTDNGKLEIDSNLCNNCGKCIESCNFDS
                     IEEKESGYKVYIGGKWGKSVRPGTQINRLFSKEELMTLIEKAILLFREQGNTGERFGI
                     TIDRIGVDKFIEMLLSDEVLERKEDILDAPLHLTGGAVC"
     gene            complement(16581..16844)
                     /locus_tag="L3M60_002091"
     CDS             complement(16581..16844)
                     /locus_tag="L3M60_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1883 domain-containing protein"
                     /protein_id="HBN6226754.1"
                     /translation="MEYSYSKMNLKKGDIVEVNLEKQANVILLDHINYVKFKNQKNYD
                     YYGGFAKKNPCRMRVPNTGTWYIVVNQDGNSGIVNFSINTIQN"
     gene            complement(16943..17188)
                     /locus_tag="L3M60_002092"
     CDS             complement(16943..17188)
                     /locus_tag="L3M60_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226755.1"
                     /translation="MFIDEELEGYILTCKISEDFKNIPEYSDEEFYVTIYKDESSDSG
                     YYALLENEEERVVWDGEVVANNIFNKLWIVVNKVKTG"
BASE COUNT         6016 a         2613 c         2216 g         6660 t
ORIGIN      
        1 tataaaatat atcataaaaa atatatacta attatatcat aaaaaataca cttaaattta
       61 atataattag tatatatttt taacaactct ctatagtaca tattaaaata ttgacagcta
      121 agttactaat aataaagctt aaaaatataa aaatcatttc ttacttttag tttacctata
      181 ataacaaaag atagatttat gttgtaataa ttaaaaatag tcaaatatat tttgatatgg
      241 ttaagtagaa ataatacttt agattaatga atattgataa gttataaaat tattttaaat
      301 tttaaaaatc actacttact aagataagtg gtatcattta acttgatact ccctattcca
      361 attgttttag gtattttact aattagaacc tatttaatat tactttatga atagtgagta
      421 gctgattaaa taataccatt tatggtttgc agccatagat taattaaatt cttaaagttc
      481 tatcctaata taattactct tcttctaaat ttttaaaatg attttcaaat aaatccaaca
      541 tatctttatc aacaacttca tcatcatgta gtgctgaaat caaactctgt aaagaattgt
      601 cgtgtatatt acttaaaaaa tcttttgttt caacacttaa atactcctta tgtccaacaa
      661 ctattgtata atgagtgtat ctatctattt tttctgcact taaaaaatgt tttttaacaa
      721 gtcttgataa aagtgtcaat gtagttgttt gtttccaacc atatttttgt tccatagctt
      781 caatagtttc ttttgatgtt actgtatcat ttttttccca aatgaacttc ataactttta
      841 attctgcttg tggtattttt gaaattgtca taaccttacg cccctctcaa acttttatta
      901 aattaatact atcgcaatat agtttttaag tcaaagattt tgtcgataat aatatattcc
      961 aattttttcc aaataaatat ttcttatata attctataat aaattacata agaaatattc
     1021 attttacatc ataaaattaa aaatataaat agtataatta tttagtctta tctttaataa
     1081 acaatccagt aaatttatac atccaaagtg cattcataaa caacaataga cttgttgaaa
     1141 aaaatactgt tctaatgcct aaatgtgctg atatttgtcc ccctaaaaat gctcctgatg
     1201 aacagcctat atactgggca gatgcattat atccaaatat tttccccaca tattcttctg
     1261 gtgcaatttt ttaagcaagc tgttcacaga aggattaagc cctgcaaccg acaaccctaa
     1321 taaaaatctt aacaacatca gttgccatgc agtttttaca aaagcttgtg gtatgaatat
     1381 aatcccagcc cataaaagtg aaattaacaa tactttttga ggtcctatct tatctgacat
     1441 ttttccaagt ttttgggctg ctaataaatt tgctaatcct gatgcagaaa atgtcaatcc
     1501 agctattaaa gctacatttg atgcaaaatt tgtgagctgt gatatatata tggttacaat
     1561 tggttctata gagtataaag ctaattgagt aacaaatgta gttacaaaaa tagatgcaat
     1621 taaaaactta tttggcaata tatctaattg ttttttaggt gtagaaattg attcttttat
     1681 ttctttaggc ttaaaattat ctactacaaa taaaatagag atgataaatg atacaaacaa
     1741 caatcctcct gttataataa atgtactttt aagacctaag attgattcta tatatcctcc
     1801 tactgttggc ccaattaaga accagtagtt gatgcagttg caagagtacc taaagcccat
     1861 cctgaatgat ttttatctgt ctgtgttgca ataagagtag tacatgcagt agcataacct
     1921 gtaaaaactc cttgaagtat tcttaatcct aataactgat aaacattttg aacaaacgcc
     1981 atggtaaata taactattcc catcccaata cttgcacgca aaagcattga ttttctccca
     2041 tatttatcag cagctttccc tcaaattggt gaaaaaaatg ctgctactac aaaagtacat
     2101 ccaaaaatta tacctgaata ctgttgtatt aaactaacat cagttacccc caattgttta
     2161 atatataatg gaagtatagg tgctatttgg ctcatcccta tagaactaaa aaacatacct
     2221 atccaacata caaataaatt tctcttccac aattccatat agaattacac cccctgtatt
     2281 ttaatttttt caataatact ataagtttat taatttatgc tattgattac tcaatatatt
     2341 catctcttct tttataatat tgtttaggaa attcataact agaaaataat ggtatcgata
     2401 aaaaatagga gtaaacatgg tgcattttct tctataaatt tatagtcctc atttaagtac
     2461 atattttttt acctaaaata tttttaatat aatcataaat taaacaatta taattctata
     2521 agatgatttt acaataaatt gccatagtta actacacaca tgtatgtaaa aacataccta
     2581 aaatattttt aattataaat acatacagac ttttatgact aaaataattt gtttcaattt
     2641 tattacaaat atacaaagta tattgttgtt aacgtattcc catagtatcc taaaactata
     2701 ttaagcacta aaaactattt taaaatactg tggaggtatc aatatggaag tatgcattct
     2761 tatgggaagt cctaagaaaa aaggcaatac tgctactatt ttaaaacctt ttattgagga
     2821 attagaaaac tataattcaa atattgaagt aatctggtta tatgattgta agatagagcc
     2881 atgtattgct tgtaaaaaat gtcaggataa cttatttgat tttggatgtt attgtaaaga
     2941 tgatgttcag aaaattttca agaaagttct tatgtgtgat ttaattgttt ttgctactcc
     3001 aatttattct tggtactgca cttctccaat gaaagcacta cttgatagat tggtgtatgg
     3061 aatgaataaa tactatggta atgaaaaagg gcctgctcta tggtcaggaa aaccagtagc
     3121 acttattaca acatgtggat ataaacctga aaatggtgct gatatttggg aaaatggtat
     3181 gaggcgctat tgtaagcatt cacagttaaa atacatagga atgcttgttg aacgtgattt
     3241 aggtaaacct aattttatga ataaagaaaa agaagaacat tctcgtcaat ttgcaaaaca
     3301 aatttattct attttagata attcaattaa agtagataat tactaaatgt gtaatgccat
     3361 aagtaaagca ttgataatat aaataaatcc tacataattt acttatttaa aataaaatat
     3421 gaagtcttta aaataaagtg tgtgttttct aaaaagtgac aataattaac atgtttttta
     3481 tcatttcaac aatgataaag aggcttacct ctattttgag atagcctctt tattactttt
     3541 tatatatcaa ttattttcat agtaaccatc atcaaaaact ccttacatat tacttctttc
     3601 aaatcctatt acttgttctt actataaatt ttttataaat tcggatattt cttcctttgt
     3661 tgcatatgaa cttatcgcac ccttaatagt tgtactacga gctgcataat aattagaaaa
     3721 ctctaaatac tcatttaacg tttcttgttt tatttctttt atatcattca atgttatttg
     3781 tttacacaat aatgtgtata aaaatgaacc tatataagaa tcccctgctc ctgttgtatc
     3841 tattgcattt actttaatac ctttgcacat accttttact tttttagtat aggcgtctac
     3901 tccatctttt ccttttgtat ataatactag ttcaacattc cctacaaaga gtttatgtag
     3961 tgatttttct atatcatttt ctcctgttat aaattctaat tcttcatcag atatcttaac
     4021 aatatgagca aaaggtaaaa attctgatat tgcattttta caagattgat cactatccca
     4081 taaaggtaat ctaatattag ggtcaaaact tattatacta ttactttcaa gtgcatattc
     4141 tattgctttt ttatgtgcta atttcattgg actatctatt aaatctactg aacaaaaatg
     4201 taggctatga caattattaa accattcttt tttaacttca tcagcttcta aaagcatatc
     4261 tgcacttgga tttctataaa atgagaaatc acgattacca tcttctttta atgatacaaa
     4321 agcaagtcct gtatttgctt tacttgttct caatacataa tcagtattta catttacttc
     4381 atttaataca tctattatat aatcaccaaa tgcatctttc cctaacttag atataaaact
     4441 tgattttcct ccaagctttg ctacaacagc actaacattt gctggagctc ctcctgctac
     4501 tcttctaaat cctgatacat tttttaattt acaaccattt tgattaggta taaagtctat
     4561 taaagcttct cctatactaa ttactttttt catcttttct accccttata atatgttttt
     4621 ataattcaat accttgcttt aatttgaatt tagtatactt atagcatact attttatcac
     4681 tataaatctt tatttgattg gaattttctt tagcaaatat tctactagta aatactgctt
     4741 ctccattatt tataaaaatt tctaaaatac ttctatctgc taatactttg atgttagtat
     4801 tttcatcaat atttatatat tcttttctat ttgttccata ctcaacactg aaaacttttt
     4861 taaagttgct tctgtctaaa gttacagtgt tttctacctt attaaattct aattttacac
     4921 cttcatcttg tgacgaaaat aattccaatc caaatttatt gctgtcaata ttttttatat
     4981 taatattaag ttcatataca ttttcatcat tttcgataat attaatattc ttttttatag
     5041 tattttgtcc acttatttca tcatatctta atttaactaa ttcatctaca ggcatttgta
     5101 atagtttatt attttttata tttaattttc ttggaacagt tagacaatgt gcccatttat
     5161 ttttatctgt agggtataaa acttctccca ttccagccca tgcaaatagt atagtttgtg
     5221 agtcttttat aaatgtttgt ggtgcataaa aatcaaatcc tttttccatt tctttcatta
     5281 tatctatttc aaatgttaaa ttatctaaat ccatattgcc aatagcatat actacattat
     5341 atatattttg ataattaaat cctttaggtt ctaatccttg tggtgaaaat attaatatat
     5401 cttttccatc tatattaata taatctggac attcccacat gtaaccaaaa tcttcatcta
     5461 tattttttac atttaattca cctttaaaat tccaatctat agagttttta gattcatata
     5521 caattatagc tcctttttta ttacttgttt gagctccaag tagcataaag tatttatcct
     5581 ttcttttaaa tacttttggg tctcttacat gtcctgtata tccttttggt atttcactta
     5641 ctattggatt attttcatat ttagttattt taccatcctt gtccattata gctaaatttt
     5701 gatatgcata tctatcttct gcactatatt ttatatttcc tgtataatac atatgcaatt
     5761 ttccatctac ttctatcgaa ttacctgaat atgctccatg agattcataa ctttcaattg
     5821 gtattaatgc tacatcttct ctattccaat ttacaaaatc atctgaactt acataagccc
     5881 aatgcttcat cccatgagtt acatcatatg gataccattg ataaaataca tggtacttac
     5941 cattataata tgctaatcca tttgggtcat taagtagccc atgttgtgga tgtatatgaa
     6001 atattggttt atatttatca tttaaagata tatttcttaa atcatttaat tcttcttttg
     6061 tcgattctaa tattgttcta tatttaggct ctttgtacat atttttattc cttttctttt
     6121 atagttttat ataatcttag aagtcattag tatatatcac tttttatagt tatttgatta
     6181 tttataattt attaattaac tgattttttc tgtaattttc caagtaatat tgttgttatc
     6241 atagctacaa ctattgttac taaatgtgct aatatatatt gaacatgtcc tccaccttga
     6301 gtagacacta ttgctaaccc tggtaccgct gttgctccaa atgccaatgc tgttactttt
     6361 gtcatatata tgtatgcacc acctattgct ccacctatac accctgctat taatggatat
     6421 ttgtacttta aagtaactcc aaatatggct ggttctgata ttccaaataa tactgaagca
     6481 aatgaagata ttcctatttg acgagctttt atatctttcc aatttaatac taagtatcct
     6541 aatatagctc caccttgacc tattaacgct actgacataa gaggcattat aaagttagtt
     6601 cctgtatctg ctataagttg tgcttcaaca gctcctataa catggtgaag tcctgttata
     6661 actataactt gctgtactcc agcaaatatc atatatccaa atattcctaa gttttcagtt
     6721 atccataata aagaggatgt tactatattt gctaagcctc ttcccaatgg gccaactata
     6781 acaaatagta aaaatgatgc tactataact gtacatattg gtgctaagaa taacttaagc
     6841 atatcatgta tttttgagtt aaagaattta tctagtttag ctactacaaa ccccatcata
     6901 agagcaacta ttattcctcc ttggaatccc accaactcaa ctttcaatcc aaataaatct
     6961 attatttctg gtactacact accatttcct actgagtatg catttgtaag ttctggatga
     7021 agcattatag ctcctagtat caaacctaat actggacttc ctccaaatct catagttgaa
     7081 gaatatacta ctaacagtgg caatatagta aatattcctg ttgatgctat acttataaat
     7141 ctattaagcc ctgctaattg aggatacatt tctattattg acaactctcc aaatattccc
     7201 ctttgaccta aaagccctgt aatacccatt aacaatgctg ctgctaatag tcctggtatt
     7261 atctgcacaa atacatctga taaagatttg atagcttgtt ggaatttgtt ttgtttttga
     7321 gttgattcag atttaacatc acttaatgac atattttcaa atcctgatag tttactaaat
     7381 tctttataaa cattatttac tattcctgct ccaaatacta cttgaagttg attagatgct
     7441 ataaatactc cttttactcc ctctaattct tctattgcct ttatatttgc tttattatta
     7501 ctatctaaaa caattcttaa tctcgttgca cagtgtgcta caccttttat attttgttca
     7561 tttcctatgt actctaataa ttgctttgat actttgttat aatcaattgt ttttgcactc
     7621 atctttatct cctctcgatt taattaaacc ggtttcgaaa ccggtttaat taaagactat
     7681 cataaattta ttttagatgc aatatttctt tcgaaaaagt tttcctaatt tttggaatta
     7741 tagtttttct aataaaaaaa tacacattac taatatatgt aatgtgtatc ttaattttta
     7801 tttattgtac tttcaccttc tattaattca aatttaatct cctttaatag tggttctttt
     7861 ctttcttgta ttaagtttac tatcgtattt gctgctactt tacctgcact ttcattttca
     7921 aattttattg ttgtaagttt agggcttata atagacaata catcatatcc cccaaatcca
     7981 gctacagata tgtcttgtgg tatattttta cctctcttat tgatttcctt cattgttgct
     8041 attgctatag tatcagttgc acatattatc atatctggat tttcattttc tagcatttct
     8101 ccactttttt gtatagacgt ttcttggtca aaatcagtat aaaatacttt tgcatcatat
     8161 cctttatctt ttaacccatt aagtactcca ttttttctat ttgaaccaac tgatatatca
     8221 ctttcatcta cgcctaaata tacaatgttt ttgtaacctt tagttgcaat atattgcccc
     8281 attttatatc ctgctccata atcatcattc actatgctat tgataccatc aactttttgt
     8341 ccaactacaa ctattggtat atctaacttt tcaatttcat tcttatgttc tttagttatt
     8401 tcggtaccaa ctaatattat tccatcaact ttcagtctag ctaattttga tatactatct
     8461 atttctgacc ttatttttct actagtatta ataattagtg atgtatattt taattctttg
     8521 agttcttcat ctattgccat cataatttta gattttacaa aagaatctaa acacggtgct
     8581 ataataccaa taaagtatgt ttttttagct ctcaaacttt gagcaaatgc attcggctca
     8641 tatccatatt catctattat tgtctttatt ttctctttag tagaatcact tatatcttta
     8701 ttatttagat atctcgatac agtactttta gatactcctg ctaatccagc aatatcattt
     8761 attgttattt ttttcatagg ttatcacctt ctacatttga tatattttat ttcattatat
     8821 tatatattta aaataaaata tagttattta tctcttcaat gctttttata cattacttgt
     8881 actatctata caacacttag aaaagtgttt ttttcactaa gttataattt ttatttttgt
     8941 tgttatattt aattatatct actataaatt gataaatatc ttaaattatt tatcacataa
     9001 aaaacttgta aaaaaaaaat atatgaaaat aaggctatga ttttcactgc catagcctta
     9061 tcaataaatg cttaatctcc atttaaaaaa taaattctta aattataaca tagtattgcg
     9121 gtcaaatatt tattattcta gaattatcag taactttata ttaattattt tgccttattt
     9181 atataaaatc tatttttata ttatttttta aataatagca taactaaaat agttttatca
     9241 tataatttga aaatttcgac tattgcaaaa cttttatcat catcttacca ccatcaaata
     9301 tgtattcaac catattattt tcatataaat aagttatata agaacgtatt gtactcccaa
     9361 ctaatacata ttgattagca tcaagcttga cattgtacaa atcacacatc ttttcaataa
     9421 ctttatcaat cataactggc tcacaacaaa cctttttaat attatctatt atttcttcca
     9481 tcttcttctt attaatatca atcagatttt ttatatctgt agttttgaca gagtgacttg
     9541 gaacaaagaa gtctgcctct aacattctca atttttctag tgtcttaaac tgtgaatcta
     9601 tatccaatag aaagaaaaaa tgatatttag taattatatt ctctggtgta agactatctg
     9661 caacaaacaa aacattatct ggagttttaa caccaatcat ttcaaaataa tgacctggaa
     9721 gagaaatcgc ttcaagttct gtatctacaa ttttgccatt tgaaggtata atataatcaa
     9781 cttcactttc ctttgctaat aaaaatttat tcttcagtgc tttacttgga taaccaccat
     9841 ataaaaatgc tgattctaat attggatttt ctgtaaaata tccttcaatt ttagttgtgg
     9901 caatcttgca ttttgtttga ttcttcagat atgcatttcc tcctatatgg tctgcatttg
     9961 aatgagtgtt tattattaat ttaggaatca aattatgttc ttctaataat cttaaaactt
    10021 gtctagctgt atccttattg tttccactat caattaaaat tgcatcttta ccatctacat
    10081 atacacctac tattgtaggg agttgaatat aatatgtatt tccttttata tgttctagag
    10141 ttaatttagc cattttataa cctcccttta aatttacttt taattatagc attaattttc
    10201 tgaaaattcc aatcaaatta tttttataat atatttttat ttcttttatt aaaaattgga
    10261 aaaactatat aatatatatt ataatctata tataaggagt gatgttattg aaaaaagata
    10321 gaataacttt ttcagaaatt ttaatattaa ttgtaggctg tatattaatg gcaatttctc
    10381 ttaacttatt ttttaaccca catgcaatcg ctgctggtgg tataacaggg cttggagttg
    10441 ttttaaattc actattcggt gtagaacttt ggattgtcaa tttactttta aatgttccat
    10501 tattcatatt tgcttacaaa atactttcaa agaaagattg ttttaaaaca gttttaggaa
    10561 taatttttct taccatagca cttaagctta ctgcgaatat ggctacatta gatataacaa
    10621 acgatatgta tcttgcaatt atatctggtt ctattttaat gggagttggt caaggtctta
    10681 tttttagaat aaatggctct acaggtggaa ctgatttaat ggcactccta ctaaataaat
    10741 attttcctac atttagtata cctgttttaa tgggtattgt tgactgtgtt gtagttgttt
    10801 tatcaggtat agtaaataga caggttgaaa ttgcactcta ttcaactgtg gctttatata
    10861 ttttagtaaa agttagtgat ttacttattg aaggatttaa ttattccaaa tcattcacta
    10921 ttatatctga cctttcaaaa gatataagta aaaaaataat ggaagattta gatagaggtg
    10981 caactattct aaaaggtgaa ggtgcatata ctggagaaaa taaaaatgta cttttagtag
    11041 tagttgaaaa gaaagaagtt gtcgaattaa aaaaattagt aaaaaatgtc gaccctgatg
    11101 cattcattat aattacagat atacatgagg ctttaggtaa cggatttaaa aaaatagaat
    11161 aattaacaga caaatagcaa gtataattac atatataaat gcaacttttt atatgtcgta
    11221 ttacacttgc tatttatata ttataaaact acaaaacaat ttaaaccata tagtggcaat
    11281 atgtttaaat tataaaatat tcatgtagtt atataataaa taatttataa atatagccaa
    11341 attggtctaa atacatatat aatatagcta atatacttct tatacttgat accatcaata
    11401 aaactaaaaa tataaaaagg agaacttaaa atgagtgtta gtttacaaaa aggacaaaga
    11461 gttagcttag taaaagacaa taatcctgta aaaaatctaa ttgtaggatt aggatgggat
    11521 atgaataaat tgggcaaaaa aaattacgac ctagatgcat ttgcaatagc attgactaac
    11581 caagataaaa tgaacgctag aggtgactta gtttattttg gaaatcttaa acacccttcc
    11641 aaagctatta tacacactgg agataactta actggaaaag gcgaaggtga tgatgaacaa
    11701 ataattgtta atttagaaaa catacctgaa tatgtacata aaatagtttt tggagtttct
    11761 atatacaaag ctaaaaaaag agaccaggat tttggtcaaa tgaacaactc ttttataaga
    11821 ctaattgatt caaattcaaa acaagaatta tttaaataca atcttcaaga agacttttct
    11881 ggtaaagtaa cagttttggc aggagaaatt tatcgtagaa atgaagaatg gaaatttaat
    11941 gctttaggaa ttggtcaaaa tgaagagctt cgtgaattaa ttaatacata taagtaataa
    12001 ttacaataaa aatttacaat gaggagttga tttaaatggg tataacttta gcaaaaggac
    12061 aaaaagtaag cttaacaaaa agtaaccctg gcctaaaaaa agtaatagta ggactaggat
    12121 gggatattaa taaatatgat ggtggttttg atttcgattt agatgcttct gcatttttaa
    12181 ctggaactaa tggaaaagta acaaatgatg gagattttat attctataat aatttaaaac
    12241 atgtttctgg agctgttgaa tatatgggtg ataatagaac aggtgttggt gatggtgatg
    12301 atgaacaaat aaatgtagac ttatctaaga taccccaaaa tattgctaaa atatcattta
    12361 gtgttactat aaatgaagca attacaagaa ggcaaaattt cggacaagtt gaaaattcat
    12421 atataagaat atacaacgaa gaaactaatg aggaacttat taaatatgaa ttgggtgaag
    12481 attttagcat agaaacagca atagttgtag cagaacttta tagacataac ggagagtgga
    12541 aatttaatgc tttaggttct ggatttgaag atgggcttgc aggattatgt aaaaattttg
    12601 gagttaatat aggctaatgt aatatatcta aataaaaata agagtatatt aatatactct
    12661 tatttttatt tagatatatt ataaactgtt tacactccta ctcttctttc tagcttgttt
    12721 ttttccatat tttactgaaa tatcaaagaa tgctgaaata ttttctgtaa aaaacttact
    12781 tctatgatat gcaatatcaa atgttctatg taaattaata ccttcaatat cacaagccca
    12841 caaaagatgc ttttttaaat aatcctcaat aagtaactca gatataactg caatgccaaa
    12901 attatctaca actgctctta atatagcttc agagttataa catgaccatc taatattcat
    12961 tgggattttt aattctttta attgtttttc taattgtgct cttgtaccac ttcctaattc
    13021 acgcataatt agtggttgat ttgaaagttc tgaaactttt atagagtctc ttttgtagaa
    13081 ctcatgctta tgtgaacata taagtacgag attatcatta ataatgcttt ttgtaacaat
    13141 atccgggtgc ttaatttttc cttctacaat tgcaatatct aattcactat taagtaattt
    13201 ttttgcaatc aatcttgtat cttctacaaa tacttcaggt tctataagag gatttacctt
    13261 gtataattct aacattattg ggcttattag acatgtacca acagttactg ttgcccctac
    13321 tcttatacgg atattttttg cacaatgctt catacaatca ttcatttctt catttacagc
    13381 taaaagatgc ctcccatatc ctagaagttt ttttcctgcc tcagttaaat ataatttttt
    13441 tgaaattcta tcaaataact taacaccata ctccttttct atttctgaaa tagcttggct
    13501 aactgatgac tgggtaatat acatatttct tgctacttca ctcattttac cacattcagc
    13561 aaccttaaca aatatctcaa gactacgaat tgtcatatta tcacaacctt agcaaacatt
    13621 agtaaatgct tatgatttta ttaatatttt agtattttac aaatacattg tcaagaagta
    13681 taattttatt atagagttat aaagtagatt ggagtgtgat atattgtgaa agtattaatt
    13741 ataggtggag ttgctgctgg aacaaaaact gctgcaaaaa taaaacgtga aatgggtgat
    13801 aattgttctg ttacaatact aaataaaggt gatgatattt cttatgcagg ttgtggtctt
    13861 ccatattacg ttggtaaggt cattgaagat aaatcatctt taatcgtaaa tacgcctgaa
    13921 agtttttcta aactaacagg agcagaagtt cgttgtggag ttgaagttac atctattgat
    13981 agaaatgcaa aacaagtaac tttgaaaacg ctttcaacag gagaaaaaga aaatctaagc
    14041 tatgataaac ttgtaattgc tacaggggcg gatccaataa aaccaccaat acctggaata
    14101 gatttacctg gagtattctt tatgcgtact ccaaatgatg ctataaaact aagagattct
    14161 attgaaaatg ttaaaagagc tgttgttatt ggtggaggtt tcattggtct tgaaattgct
    14221 gaaaaccttt ctgcaatggg tatacgtgta agtgttattg atatggctga acatgttatg
    14281 cctggatttg atactgattt tgctgaatat gttgaaaatc atatggctga taaaggcata
    14341 atgattttta caggtgacca ggttgtgggt attgaaggtg aaaataaagt tgaaaaactt
    14401 cgtactaaaa atagagctat taaaacagat ttagtagtta tgtcagttgg aatccgtcct
    14461 aacacaggtt ttctaaaaga cacaggttta gaatttgctc ctaataagac acttattgca
    14521 gatgaatata tgcttacaaa tgataaagat atatacgtag ttggagattg tgcttttgta
    14581 aaaaattctt taacaaataa acctacatgg tcacctatgg ggtcatctgc aaatcatgaa
    14641 ggtagaattt gcgcacaaaa tatatctgga aaatctaaaa cttacaatgg tgttcttgga
    14701 acaacagtag taaaattacc tgagctcaat gcaggaaaaa caggtctaag taaagaaata
    14761 gctgaaaaag agggttataa tgtttgctct gtaaccatag ctactgatga taaagctcac
    14821 tattatccag gtgcatctaa ttttattata agaatggttg ctgaaaaaaa tactagaaaa
    14881 ttattaggtg ttcaagttat gggacctgga gcggttgata aaatagtaga tatcgctgct
    14941 actgcaataa ctttgggagc tgacctttat tcattagaaa gtatggacct tgcatacgca
    15001 ccaccttttt caactgctat tcaccctttt gtagtggcag taaatgtttt acaaaataaa
    15061 cttaatggag aacttgatag tgttttactt gacgaaattg aagattttga cagttggact
    15121 aaacttgatg tttcaaaagc tccttctatt cctagtttgc gttatattcc tgttggtgaa
    15181 attaacggag aaattgaagg tcttgctaag gatgaaaaaa ttcttcttat atgtgctaaa
    15241 ggtaaaaata gctacatggc gcaaaatcgc ttaaaacgct ttggctatac aaatacgaaa
    15301 gttcttgaag gtggaatact attttatcca aatcttaagg taatggagga gtaaatatga
    15361 gtaatgttac agcagaacaa aaaaaggaag taaaaggtag aggttttcta ccaagcagag
    15421 atggagaaca ttttgcagca cgtataataa ctgtaaatgg tgtaataaat gcatcacaag
    15481 caaaaaaaat tgcagaagct gctgaaaaat tcggaaatgg tcaattagca tttactacta
    15541 gactaacaat agaattgcct ggtgtaaaat ttgaagatat tgaagcttta agtgaattta
    15601 tcgctagcga aaatttaata acaggtggaa ctggctcaag ggttcgtcct gttgtagctt
    15661 gtaaaggtac tgtttgtgtt catggtctag ctgatacaca ggcacttgct gctgaaatac
    15721 accaagaatt ttacaaaaat tggtacgatg taaaactacc tcataaattt aaaattggtg
    15781 ttggtggatg ccctaataat tgcattaaac ctgatttaaa cgatcttgga attgttggtc
    15841 aacgtgtacc tgattatgac tcagaacttt gtgttggttg taaaaaatgt gctgttgtag
    15901 aagcctgtcc tgttaaagca gctaaattaa cagataatgg taaacttgaa atagatagta
    15961 atctatgtaa taactgtggt aagtgtatag aaagttgtaa ttttgatagt attgaagaaa
    16021 aagaatctgg ttataaagtc tatataggag ggaaatgggg aaaatcagtt cgtcctggaa
    16081 cacaaattaa tagattattt tcaaaagaag aacttatgac tttaattgaa aaagcaattc
    16141 ttttatttag agagcagggt aacacaggtg aacgttttgg aataactatc gatagaatcg
    16201 gtgttgataa atttatagaa atgcttttat cagatgaagt tttggaaaga aaagaagaca
    16261 tacttgatgc tcctttacac cttacaggtg gagctgtatg ctaacaataa atattttagt
    16321 tgtataaata tcattaacat attgaaaaaa ttttagttaa gaataatcaa aattcttatg
    16381 ataaatgagg ctatctcaaa acacagataa gcctcttttt cattgttgaa atgataaagt
    16441 atatttgagt ataaaaaaga gtgtctcata aactataaaa ttcattttga ggcacccaaa
    16501 ttattttata ataattgctt attaatttta ttttaaatat ataaaatgca caaaaagtaa
    16561 gaatatctta tattctaaaa ttaattttga attgtattga ttgaaaagtt tactattcca
    16621 ctatttccat cttgatttac tactatatac catgttcctg tattaggaac tctcattcta
    16681 catggatttt tctttgcaaa tccaccatag taatcataat ttttttgatt tttaaatttc
    16741 acataattta tatggtctaa caatattaca ttggcttgtt tctctaaatt tacctctaca
    16801 atatcccctt tttttaggtt catcttacta taactatatt ccattgaatt actcctccag
    16861 tatcgaataa tctttgttat ttactcatct taaatatgta aaaattcatt taaaatatta
    16921 caaatactat acaattggac tattatccag ttttaacctt atttactaca atccaaagtt
    16981 tattaaaaat attattagca acgacttccc catcccatac aactctttct tcttcatttt
    17041 ctaataaagc atagtaccca gagtcagaac tttcatcttt atagattgta acataaaact
    17101 cttcatcact atattcaggt atatttttaa agtcttcaga tattttacat gttaatatat
    17161 atccttctag ttcttcatct ataaacaaag caattcctcc aatctctata tttttactaa
    17221 ttataccata ttatgtcata aactttttat ttcgttgtaa aattctattt tttctttact
    17281 ttataaacaa tttatatatt tttttagttt tttactttac attacactaa ttaattcttt
    17341 tgttttctta tatattcctt tataattcat aaatttaacc attagtttat acgactttat
    17401 atacgtaaac atataatctc gcttaatatt aaataattta tatttttttg atactataat
    17461 catatttttt ggtcagctat taatttttaa ataagattac tttaa
//
LOCUS       DAFNNB010000074        17389 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0075, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000074 DAFNNB010000000
VERSION     DAFNNB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17389
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0075"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1063)
                     /locus_tag="L3M60_002093"
     CDS             complement(<1..1063)
                     /locus_tag="L3M60_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBN6226756.1"
                     /translation="MESFIMLDMFITIVSSIIELWACKKVFDYTSKVKTSLINLYFLI
                     STFIIISTFYTNVGADNRILICIFVMFVFYKLNYDVNIYKCLIVLFSYWAILVCINAL
                     SMIVIVSINNLSSMNILLNDNFYRYETIVLGNIVLIVLLYIYKFISNDLQVTKREIYM
                     CIPIVANMISFFIFYKYILKLLDISSATKLDILIMVILLTFSNIALIYSLVKILKNNK
                     KYIELTKLQEKINAENKYYSNIKKYYIKTKELNHDMKNHLICIKEMNKKNIDTSNYIN
                     NIEKKLDEYSTVFDTGNITLNIILSEKKELCIEKEIDFTININFSDCNFIKEEDTCSI
                     FANALDNAIEACEKIKNDDK"
     gene            1681..1881
                     /locus_tag="L3M60_002094"
     CDS             1681..1881
                     /locus_tag="L3M60_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226757.1"
                     /translation="MNDGYISYTFNESYTKDEDETAYCTKVTDTLIELFKIDMETQKY
                     NPQGNASLEGAEFTWKSYVGYN"
     gene            1872..2102
                     /locus_tag="L3M60_002095"
                     /pseudo
     CDS             1872..2102
                     /locus_tag="L3M60_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860758.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            2698..2907
                     /locus_tag="L3M60_002096"
     CDS             2698..2907
                     /locus_tag="L3M60_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226758.1"
                     /translation="MVKVKTKKTFVCTHMCKVEIPMSVRKLYEKVLASSIRAPNLEGM
                     IEKFDKDLPHSNDTDLIELKNAISK"
     gene            3006..5669
                     /gene="mgtA"
                     /locus_tag="L3M60_002097"
     CDS             3006..5669
                     /gene="mgtA"
                     /locus_tag="L3M60_002097"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016440611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBN6226759.1"
                     /translation="MSKATLFDSRIKKYAYCEISEIYRDIGSSPDGLSIEQIGSMREK
                     YGANSFNGRKNDTTMQRLRRAFINPFHVILFVLGIVSLVTDVFLASNFARNATTAIII
                     FSMILISGVIRLIQELRAKSAAAQLDRLVHESVTVRRGGKLIEIPGEELVVGDIVLFS
                     AGDCVPADVRLTKITDLFISQAAITGESAILEKSCRKLSYGEQETLTQLENLAFMATT
                     VISGKGEGIVLAVGKDTLYGGFTKPDSDDKDSFKKGADSIAWVMIRFMAILVPIVFIL
                     LGITGGKWMESFAFALSVAVGLVPEMLPMVITACLAKGSLAMSKKQTIIKNINAMQSF
                     GSMDVLCMDKTGTLTNESILLEYYMDILGNENAEVLDLAYLNSSYHSGVCNTIDNAIL
                     ACNTMPGREAHYANLLTKFQKADEIPFDYARKFVSTLVQDSNGNSQLIMKGDIEHIFS
                     RCNYVEYHGKLLPMGQDARQSVSSVVGEMLQDGMKVIAVARKNVGAQKKITPDDEWGM
                     TLVGYLSFFDAPKQTAKESVTALKRLKVIPKILTGDQAAIAVSVCRRVGISTERILTS
                     TQLDEMTDCELKKAVEHTHVFAELTPGHKVRLVSAMQDNGHSVGFLGDGVNDIPALNE
                     ANVGISVDTAVDAAKDAADVVLLQKDLNVLEQGVMEGRKTFTNMLKYIKITASSNFGN
                     IFSIICASAFLPFLPMTSIQILLLNLLYDTLCIVLPWDNVDEEEMLSPRDWSGKTLRQ
                     FMLSFGPISSLFDIVTFLFLYYFLCPMLCGGATYLQLTDPSLKLQYVALFQTGWFLES
                     MWTQVLILHFLRTSKIPFFQSRASAPVISITLVGIIGFTYLTFAGGASLFGLTKLPLW
                     YFVFLLLVVFLYMLMNSVAKYFYKKKYQELI"
     gene            5685..6188
                     /locus_tag="L3M60_002098"
     CDS             5685..6188
                     /locus_tag="L3M60_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN6226760.1"
                     /translation="MKKNISYIPMDSALEIWLLEKLLRDPPDSDYAKDLLSGIQEMIR
                     GEVSSLLLGSSEESDCPVGTLSDRILAGDLEIYPRGRKVLIKGSEVNLTPKEFDILYF
                     LVQNKGEVFTKEQIYRAVWEEDYLLDNSNIMAFIRKLRKKIEPAPDSPTYILTIWGIG
                     YKFNDQI"
     gene            6572..6883
                     /locus_tag="L3M60_002099"
     CDS             6572..6883
                     /locus_tag="L3M60_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3784 domain-containing protein"
                     /protein_id="HBN6226761.1"
                     /translation="MDFTCIFFGILFTFAGFWLAVGKGYRHLSLWKNMPKEEKDKINI
                     VPLSRNVGGIIALNGIIFLMKGVLPGFSNHWFIFSIIAWLIVASFDVWYIEKSVRYRN
                     Q"
     gene            7048..9813
                     /gene="mgtA"
                     /locus_tag="L3M60_002100"
     CDS             7048..9813
                     /gene="mgtA"
                     /locus_tag="L3M60_002100"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002571344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBN6226762.1"
                     /translation="MNKKENRMAVRQTVEKTVIRDEQNRRIQFAATNPIKEVLKKFHT
                     TPRGLEVDAIPVSRAKYGTNKVTHEKKKSLAKRLAGAFINPFTAILFCLAVVSTITDM
                     IFPYFSLFGSVPEDFDYLTVVIILTMVFISGTLRFVQESRSGNAAEKLLAMITTTCTV
                     TRRNQEKKEIPMDDLVVGDIVHLSAGDMIPADVRILDAKDLFVSQASLTGESEPIEKT
                     PNVSAQKESVTDYTSIAFMGSNVISGSATAVVVCVGDHTLFGSMASAVAGEAVETSFT
                     KGVNAVSWVLIRFMLIMVPLVFFVNGITKGDWLEAFLFGVSIAVGLTPEMLPMIVTTC
                     LAKGAVSMSKKQTIVKNLNSIQNFGAIDILCTDKTGTLTQDKVVLEYHLNVNGEDDTR
                     VLRHAYLNSYFQTGYKNLMDLAIIHKTEEEEATDSRLTDLSENYVKVDEIPFDFTRRR
                     LTTVVQDKGGKTQMVTKGAVEEMLSICAFAECNGEVQPLTDDIRYRILETVANLNDKG
                     FRVLAIAQKSNPSPIGAFGVKDECEMVLIGYLAFLDPPKESTADAIKALKDHGVITKI
                     LTGDNDKVTRTICKQVGLEVRNMLLGSDLEQMGDAELARVADSTDVFAKLTPDQKVRV
                     VSVLRENGHTVGFMGDGINDAAAMKAADIGISVDTAVDVAKESADIILLEKDLMVLEQ
                     GIIEGRKTYANMIKYIKMTASSNFGNMFSVLAASALLPFLPMMSVHLIILNLIYDLSC
                     TAIPWDNVDEEFIAKPRKWDASSVGSFMIWIGPTSSIFDFTTYIFMYFVFCPLFVSNG
                     VLFNDLPAHFSGAELAMLQAQYIGMFQAGWFVESMWSQTLVIHMIRTPKLPFLQSRAS
                     APVTLLTMTGITVLTIIPFTVFGTMLGFVALPATYFAYLIPCILLYMILATSLKKAYI
                     RHFGELL"
     gene            9826..9993
                     /locus_tag="L3M60_002101"
     CDS             9826..9993
                     /locus_tag="L3M60_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002587909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226763.1"
                     /translation="MNWKELWMTLFGTTEWFGLNIGFWVAMAVVLLIVIVMNVVFWSM
                     KPKGDAKESYK"
     gene            complement(10663..11238)
                     /locus_tag="L3M60_002102"
     CDS             complement(10663..11238)
                     /locus_tag="L3M60_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6226764.1"
                     /translation="MSKIIAFVGSPRKNGNVDTIINEIIKGANKNKVDVKKYYLNDMN
                     IKGCQGCLYCRKNHSCSLKDDMIQVYEDIKKAEYVIIGSPVYICQVSAQTKLLLDRLY
                     PLTTIDKGKHIPRFGNKRIIMVYTQAAPLPIFFKRYFRYTSKHLKGMGLNHYKTIIAT
                     KAFEKNSIENNPKILKKAYNLGFKISKDINV"
     gene            complement(12176..12496)
                     /gene="rhaM"
                     /locus_tag="L3M60_002103"
     CDS             complement(12176..12496)
                     /gene="rhaM"
                     /locus_tag="L3M60_002103"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBN6226765.1"
                     /translation="MNTVHKCFKMKLFKGFENEYKKRHDELWSEMIDMIHEYGGSNYS
                     IFLDEETNYLYGYIELKDAKLWDKSSETAICKKWWNYMSDIMETNEDNSPISIELKSM
                     FYLK"
     gene            12682..13149
                     /locus_tag="L3M60_002104"
     CDS             12682..13149
                     /locus_tag="L3M60_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HBN6226766.1"
                     /translation="MKDKTLEDILKQKTQAERYILECIDTNNVKKLDNKKIMSILHTI
                     LCWEEVNGTIVLTDKNILSLDEDIMVSKHHRFIDIPKHKHNFIELIYVYSGSCTQIID
                     GKETILKEGEICLLDMNVEHSINAVKENDIIINCIMRREYFDASFFLEFRLMS"
     gene            13233..13745
                     /locus_tag="L3M60_002105"
     CDS             13233..13745
                     /locus_tag="L3M60_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226767.1"
                     /translation="MKRLVAELTKEYLNKSMCYKEIMDCYIILIFYELLEIHKTKNEK
                     TLTSDSKINIDNIAEMMKYIEGNYAQTTLREVAKKFHFHPNYLSTLIKKTTGKSFKDI
                     IHEQRLKKATTLLKSTDMSIEDISIEVGYNNQTFFYKKFKETYSLTPKKYRIKSRILL
                     KEVSHETIYS"
     gene            13726..14210
                     /locus_tag="L3M60_002106"
                     /pseudo
     CDS             13726..14210
                     /locus_tag="L3M60_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895448.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            14189..14521
                     /locus_tag="L3M60_002107"
     CDS             14189..14521
                     /locus_tag="L3M60_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HBN6226768.1"
                     /translation="MYVLLSKYFTQKELAKKQLLNYTALLSFEIDAKDVNDIKKFVNK
                     LDLFKIGVSWGGHESLVFAPNIAILNEMTLEQAKALNVSPYTIRISLGLENSEDLIND
                     LNDALSIL"
     gene            15203..17161
                     /locus_tag="L3M60_002108"
     CDS             15203..17161
                     /locus_tag="L3M60_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HBN6226769.1"
                     /translation="MKRAYYSSSVNEFIGKDIFTIFGEITCNDQFEANDLQKNTWKKE
                     IEILKRELSTFKDGHLLFEYTIPRIGSRIDNVLIYKGIVFLLEFKVDEDTYPRYAIEQ
                     VTDYALDLNCFHKESHDKLLVPILICTKAPQRNQRIRLMKENILETYCSNEFNIGKYI
                     EEVCTTYNKSSFNPDAWMDSLYMPTPTIIEAAQALYMGHNVEDISRNDASAKNLNQTT
                     KAINKIIDYSKIHNKKSICFITGVPGAGKTLAGLNIAIERQKVDENEHAVFLSGNGPL
                     VDVLQEALARDDVTRNGTRKTEAIRKAKEFIQIIHHFRDEAISVDTPPIERVTIFDEA
                     QRAWDEPNLTNFMKRKKGVLDFNMSEPEFLISILNRHIGWATIVCLIGGGQEINKGES
                     DGISGWFASLRNNYSDWDVYISNKIIDEEYSKGNSFDELTEGVNYRIIDDLHLSVSLR
                     SFRSEKVAAFVKAVLDVDKIAANELYNQFKKDYPICVTRDLELAKKWVRDKSKGSQRY
                     GMTASSGAKRLRKYGVWVQSKVDAKNWFLNGKDDVRASYFLEETATEFDIQGLELDWT
                     IVCWDGNLRFKNEQFEYYNFNGTKWQNINKEDNILYLKNAYRVLLTRARQGFVIFVPT
                     GDESDITMQPSFYNGVYEYLKEIGIEEI"
BASE COUNT         6022 a         2427 c         3071 g         5869 t
ORIGIN      
        1 ttttatcatc atttttgatt ttttcgcaag cttcgattgc attatctaat gcatttgcaa
       61 atatagaaca tgtatcttct tccttaataa aattacaatc actaaagttt atattaattg
      121 taaaatcaat ttctttttct atacaaagtt cttttttttc tgataatata atgtttaaag
      181 taatatttcc tgtatcaaat acagtactat attcatcaag ctttttttct atgttattta
      241 tgtaattact ggtatcaata ttctttttat tcatttcctt aatgcaaata agatgatttt
      301 tcatatcatg gtttaattct tttgtcttaa tataatattt ttttatatta ctataatatt
      361 tattttcagc atttattttt tcctgtagtt tagttaattc aatatatttt ttattatttt
      421 ttaatatctt aactaggcta taaatcaatg ctatgttaga aaatgttaat aatatcacca
      481 taattaagat atctaattta gtagcggaag aaatatctag aagttttaag atatatttat
      541 aaaatataaa aaatgatatc atatttgcta ctattggtat acacatataa atttctcttt
      601 tagtcacttg caaatcatta gatatgaatt tataaatgta taacaaaaca attagaacta
      661 tatttcctaa aactatagtt tcgtatctat aaaaattatc atttaataaa atattcatag
      721 aactcaagtt atttatcgaa actattacta tcatacttaa tgcatttatg cacaccagta
      781 ttgcccaata agaaaacaaa actatcaaac atttgtatat attaacatca taatttaatt
      841 tataaaaaac aaacattaca aaaatgcata ttaatattct attatcagca ccaacattag
      901 tatagaaagt agaaataata ataaaagtgg aaattaaaaa atataaattt attaaactag
      961 tttttacttt acttgtgtaa tcaaacactt tcttgcatgc ccatagttct ataatagaag
     1021 aaacgattgt tataaacata tctaacataa taaaactctc cataacttaa acaattttgc
     1081 ataaatttcc tttaataaca cgaaaatgat ttgaactttt ataataatta tatcataaaa
     1141 tagattatac ttggatgaag ttgctaaata ttatatatat ataaataatt ttatctacat
     1201 atgtaattat taatataaat gagtaaaact ataatatgca ttgtaataat aaaacgaacg
     1261 aaacgaacga aaattatcat actaaaacat aagacatgtt ataatcatgt tagagaaatt
     1321 cttgaatata cactaaaagt actctttcta taggagtgct tctttcattt ctccaaacaa
     1381 tattatctac ttcatcaatg attattaaat caatctcgct ataaaataat aatagataat
     1441 gaagaatatt gcgttttaag agaaaagtac taaaataaag caagatgttc acttcaaaat
     1501 aagtgtctat ttttttaccc aattacaaaa tgaaatgagt atttagaatt atgaaaaaaa
     1561 tattaaagcg attgtgtatg ggcgttcttg cctttgctac tgtcattacc agtggttcat
     1621 gcgtctgata aatagtattg gacagaatcg acagggcgta tcgggattgt tgaaaaagta
     1681 atgaatgacg gttatatcag ttatacattc aatgagagct acacgaaaga tgaggacgaa
     1741 acagcgtatt gtacaaaagt aacagatact ttgattgaac ttttcaagat tgacatggaa
     1801 acacagaaat acaacccaca aggaaacgct tctttagagg gagcagagtt tacatggaaa
     1861 tcttatgtag gctacaactg aatgatacca acgggattaa aaacttcgtc aaaaactttg
     1921 taaagttcta ctacacttgg aatagtagca aagaagctat cgaagtaagg atgtagacaa
     1981 tcagcggtta tctgacaaag gaattgcagg acttgaatgt agataccacc attagaacgg
     2041 atataccgac cagctacaca gttacagatg agattgtatg gagtatcgag aagttgggta
     2101 caatgtgaaa gttaaggtgc tgtgaaattc attgacaatc aaacaaaggc aatgtaggtg
     2161 tcgtagtttg aacttgtggg acataaagac agtaactgaa atattgtggg ataattattt
     2221 aaagtatgca tttctatata aatttacacg cctttgttaa gaacattatt ctttatattt
     2281 atgaaagaca tcttaaacaa agtactaatg gcttcgtaaa attcctttct taatatattt
     2341 ggattagaaa aatcgcatct taactgtaga ctttctatac atatgaatta tgccagtcaa
     2401 catatttaaa atttctgttg ttttattttg ttgtaaatag caataataga taagagtaat
     2461 caatagtgaa gtggaattat taaaaatact attggttttt tctgttccgt tccaatttac
     2521 gcattgtatt gttttataaa ctacagagtg gttaattatg tagttcagat attccataca
     2581 aactaattgt attgctttta cactcgtttt ctatactgcc acttttgcca ttctatcact
     2641 cataatacat atatcactat gagaaacttc tctaagtagt taatttaaat ttgatacatg
     2701 gtcaaggtga aaaccaagaa gacgtttgtc tgcactcata tgtgtaaagt ggaaatacct
     2761 atgtctgtgc gaaagctcta tgagaaagtg cttgcttcat ccatccgtgc tccaaattta
     2821 gaaggaatga ttgaaaaatt tgataaggat ttacctcact ctaacgatac ggatttgatc
     2881 gagctgaaaa atgccatatc gaagtaaatc gcaagaaaaa agcaagaata taaatatagc
     2941 atttttagaa cactgtgtta taatgtggat atctttattt atatttataa aggaggggat
     3001 gtttcgtgtc aaaagcaaca ctatttgata gtcgtattaa aaaatacgct tactgtgaaa
     3061 tatcagaaat ctatcgagac attggctcat ctccagatgg tctatctatt gagcaaattg
     3121 gttcaatgcg ggagaaatat ggagcgaaca gcttcaatgg gcgaaaaaat gatacgacga
     3181 tgcaacgatt gagacgagca tttattaacc catttcatgt gattcttttt gttctgggca
     3241 ttgtttctct tgttacagat gttttcttag catccaattt tgcgagaaat gctactactg
     3301 ccattattat cttttccatg atactaatta gtggcgtaat tcgtctgatt caggagctac
     3361 gagcaaagag cgctgctgct caacttgatc gcctagttca tgaaagcgtc acagtgaggc
     3421 gcggtggaaa gttaatagag attcctggcg aagaattagt ggtaggggat attgtactat
     3481 tctccgcagg tgattgtgtt ccggcagatg tcagattgac aaagattaca gatctcttta
     3541 tctctcaggc tgcaatcact ggtgagagtg ctatccttga aaagagctgt cgcaaactca
     3601 gttacggaga acaggagacg ctgactcagc ttgaaaacct cgcttttatg gcaaccacag
     3661 tcatcagtgg aaaaggggaa ggaattgtgt tggcagtagg taaagatacc ctgtatggag
     3721 gatttaccaa accggattct gatgataaag attcatttaa aaaaggagct gattctatcg
     3781 cctgggtgat gattcgtttc atggcgatat tagttcccat tgtatttata ttactgggaa
     3841 tcactggtgg aaaatggatg gaatcctttg cttttgcctt gtcagttgct gtaggtctgg
     3901 tgccagaaat gcttcctatg gttattacag cctgtctcgc caaaggaagt cttgctatga
     3961 gcaaaaagca gactattatc aaaaatatta atgctatgca gagctttggc agcatggatg
     4021 tgctttgtat ggataaaacg gggactctta ccaatgaaag tatcctgctg gaatattata
     4081 tggatatctt gggaaatgaa aatgcagagg ttcttgatct tgcttatctg aatagctcct
     4141 atcattctgg tgtctgcaat accattgata atgccattct tgcctgcaat accatgccag
     4201 ggcgggaagc ccattatgca aatcttctga caaaatttca aaaagctgat gaaattccat
     4261 ttgactatgc tcgtaagttt gtcagcacac ttgtacagga tagcaatggg aatagtcagc
     4321 ttattatgaa aggggatatc gaacatatct tttcacgctg caattatgtg gaatatcatg
     4381 gaaaacttct ccccatgggg caggatgcga gacagagtgt atcttctgta gtaggtgaaa
     4441 tgttacagga tggaatgaag gttattgcgg tggcacgaaa aaatgtagga gcacaaaaga
     4501 aaattactcc tgatgatgaa tggggcatga ctctagttgg gtatctatcc ttctttgatg
     4561 caccaaagca gacagcaaag gagtctgtaa ctgcactcaa aagattaaaa gtgattccta
     4621 agattctgac aggggaccaa gcagcgattg ctgtatctgt ttgccgtcgg gtgggtattt
     4681 ctacagagcg tatcttaact agtacacaac tggacgaaat gacagactgt gaacttaaaa
     4741 aggctgtgga acatactcat gtttttgcag agcttactcc gggacataag gtgcgtctgg
     4801 tctctgctat gcaggataat ggtcattccg tgggatttct tggagatgga gtcaacgaca
     4861 ttccagcact aaatgaagca aatgtgggaa tctctgtgga tacagcagtg gatgcagcaa
     4921 aagatgctgc tgatgtggtt ttgttgcaaa aggacttgaa tgttttagag caaggtgtaa
     4981 tggaggggcg aaaaactttt accaatatgc tgaaatatat taaaatcact gctagctcta
     5041 actttggaaa tattttttcc atcatttgtg ccagtgcctt tttaccgttc ctacctatga
     5101 cttcaattca gattctgctt ttgaatttgc tgtatgatac gttgtgtatt gtgcttccct
     5161 gggacaatgt agatgaggaa gaaatgctat ctccaagaga ctggtctggc aaaacactaa
     5221 gacagtttat gctatctttc ggaccaatca gttctctgtt tgatattgtt accttcctgt
     5281 ttttgtatta tttcctatgt ccgatgctat gtggaggggc aacctatctg cagcttacag
     5341 atccttctct gaaacttcag tatgtagctc tgtttcagac tggatggttt ttggaatcta
     5401 tgtggacaca ggttttgatt ttacattttc tgcgaacttc caagattcct tttttccaga
     5461 gcagggcatc tgcaccagtc atttctataa ctctagtagg gattattggc tttacttatc
     5521 ttacttttgc tggtggtgca tcgctgtttg gactgacgaa attgcccctt tggtatttcg
     5581 tatttttact gctggtggta tttctctata tgctgatgaa ttcagtagct aaatattttt
     5641 ataagaaaaa atatcaagag ttgatttaag aggaggcatt catgatgaaa aagaatatta
     5701 gttatattcc gatggattcg gctcttgaaa tctggctatt agaaaaacta ttaagagatc
     5761 caccagacag tgattatgcg aaggatttac tttctggcat tcaggagatg attcgcggag
     5821 aggtatcttc gttattgtta gggtcatcag aagaaagcga ttgtccagtg ggtactctgt
     5881 cagacagaat cctagcaggg gatcttgaaa tttatccaag aggcagaaaa gtactgataa
     5941 aaggttctga agtcaacttg actccaaaag aatttgatat tttgtacttt cttgtccaga
     6001 acaaaggtga agtttttaca aaggaacaga tttatcgtgc cgtatgggag gaagattatc
     6061 ttctggataa cagcaacatt atggctttta tccggaagtt gcgaaaaaaa attgaacctg
     6121 ccccagattc tcccacatac attttgacta tctggggtat tggatataaa tttaatgatc
     6181 agatatgaca aagacggact aaaaccattt tcggtttcag tccgtctatt ttcttaccaa
     6241 atcgcaagaa aatcgcaaga ctttagccat atgattctac ttggattttt tatatattgt
     6301 tagtagttaa agggaggtgt tgtgagtgag gagagaaatc aaagatttga aacaggcaat
     6361 tcttttttat ttctaccctg tatttacaat actatgtagc tatttgggtt ggaatttatc
     6421 tgaacaaaga atggcaatct ttaagatatg gtatcaggtg agtgttatga tctggtttat
     6481 tcttgctgtt actatgattg tgctcctcat taaacttatg aagctgacga aactaagaaa
     6541 caggtttaag cattgaaagg aggaacaagc gatggatttt acatgtatct tttttggaat
     6601 tttatttact tttgcaggct tttggttagc cgtgggaaaa ggatatcgtc atctttccct
     6661 gtggaaaaat atgccgaagg aagagaaaga taaaattaat attgtcccgc tttctcgtaa
     6721 tgttggagga ataattgcat taaatggaat tatattctta atgaaaggag ttttgccagg
     6781 attttcaaac cactggttta tattttcaat aatcgcatgg ttgattgttg ccagctttga
     6841 tgtctggtat atcgaaaaaa gtgtccgcta tcgtaaccaa taatccgcat tgcggtttca
     6901 catagatatt ttttaagatt gtgaggtgaa agacatggac tctgatgtca gtccacaaat
     6961 gcagagaata ttagatgaga atttggagat tccgtgtcac tcatataata ttccttgctc
     7021 ctatgaaaat tcaaggaggt aagaacaatg aacaaaaaag aaaatcgtat ggctgttcgt
     7081 cagactgtag aaaaaacagt catccgtgac gaacagaacc gccgcattca atttgcggca
     7141 accaatccta ttaaagaagt tttaaaaaaa tttcatacca cacctcgtgg attggaagta
     7201 gatgcaatac ccgtaagtcg tgctaaatat ggtaccaata aagtcactca tgaaaaaaag
     7261 aaatctctag caaaacggct agctggtgca tttatcaatc catttaccgc cattttattt
     7321 tgtctggctg tagtatccac aattacggat atgatttttc cgtatttttc cctgtttggc
     7381 agtgtgccag aggactttga ttacctgact gttgtaatca ttttaacaat ggtattcatt
     7441 tccggtacac ttcgttttgt acaggaatcc agaagtggaa atgcagcaga gaaattgtta
     7501 gctatgatta cgaccacttg tacagttact cgtaggaatc aagaaaaaaa agaaattcct
     7561 atggatgatt tggtggtggg agatattgta catctgtctg ctggagatat gattccggcg
     7621 gatgttcgta ttttggatgc gaaggatttg tttgtcagtc aagcaagtct tactggtgag
     7681 agtgaaccta tcgaaaaaac ccccaatgta agtgctcaga aagaaagcgt gactgattac
     7741 acaagcattg cttttatggg gagtaatgta atttctggta gcgcaacagc tgttgttgtt
     7801 tgtgttggcg atcatactct gtttggctct atggcgtccg cagttgccgg agaggcggtt
     7861 gaaacaagct ttaccaaggg tgtaaacgcc gtttcttggg tattgatccg ttttatgctc
     7921 attatggttc cactggtgtt ctttgtcaat ggtatcacca agggtgattg gttggaggct
     7981 ttcttattcg gagtttctat tgctgttggt ttgacaccgg aaatgctgcc tatgattgta
     8041 acaacctgtc ttgcgaaagg cgctgtttcc atgagcaaga agcagacaat cgttaaaaat
     8101 ctcaattcca ttcagaactt cggtgctatt gatattcttt gtactgataa gactggcacg
     8161 ctgactcagg ataaggttgt gctggaatac catctgaatg taaatggcga ggacgatacg
     8221 cgagtactgc gtcacgctta cctcaatagt tacttccaga ctggctataa aaacctgatg
     8281 gatttggcaa ttatccataa aactgaggaa gaggaagcca cagattctcg tctcacagac
     8341 ctgtccgaaa actatgtgaa agtagatgag attccttttg actttacacg ccgtcgtctg
     8401 actacagtgg tacaagataa aggtggtaag acccagatgg taacaaaagg tgctgtggaa
     8461 gaaatgctct ctatttgtgc gtttgcagag tgtaatggag aagtgcagcc tctgactgat
     8521 gacattcgtt acagaatttt ggagacagtg gccaatctta acgataaggg attccgagtg
     8581 ctagcgattg cacagaagag taatccttcc ccaatcggcg catttggtgt taaagatgag
     8641 tgtgagatgg tactgattgg atatctggca ttccttgatc ctccaaaaga atctactgca
     8701 gatgctatta aagctctgaa agaccatggt gttatcacca aaattttgac aggtgacaac
     8761 gacaaagtga ctcgaactat ctgcaagcag gtaggactag aggttcgcaa tatgcttctc
     8821 ggttcagatt tggaacaaat gggcgatgca gagcttgcaa gagtggcaga ttctacagat
     8881 gtatttgcga agttaacccc cgatcagaaa gttcgtgtgg tttctgttct ccgtgaaaat
     8941 ggtcatacgg tcggtttcat gggtgacggt attaatgatg ctgctgctat gaaagctgcc
     9001 gatattggta tttcggtaga taccgctgtg gatgtggcaa aagaatctgc tgacattatt
     9061 ctattggaaa aagacctgat ggttttggaa cagggtatca ttgaaggacg aaagacatat
     9121 gcgaacatga ttaaatacat taaaatgact gcttcatcca acttcggcaa tatgttttca
     9181 gtgcttgctg catctgctct gttacccttc ctaccaatga tgagtgttca tcttataatt
     9241 ctaaacttaa tttatgattt atcctgtacc gcaattcctt gggataatgt ggatgaggaa
     9301 tttattgcca aaccacgtaa atgggacgct tccagtgttg gaagttttat gatctggatt
     9361 ggaccaacta gctctatctt cgattttaca acatatattt ttatgtactt tgtattttgc
     9421 ccattgttcg tatcgaatgg tgtgttattc aatgatttgc ctgcccattt cagcggtgca
     9481 gagctggcaa tgcttcaggc gcagtatatc ggaatgtttc aagctggatg gttcgtggaa
     9541 tctatgtgga gccagacatt ggtcattcac atgattcgta ctccaaagct gcctttcctt
     9601 cagagtcgtg catcagctcc ggtgactttg ctaacaatga caggaattac ggttctgaca
     9661 attattccgt ttactgtgtt tggaactatg cttggatttg ttgcattgcc tgcaacatac
     9721 tttgcatatc tgattccatg tattctgctg tatatgattt tggcaacaag tttgaaaaaa
     9781 gcctatatcc gtcatttcgg cgaactactt taaggaggtg caatgatgaa ctggaaagaa
     9841 ctttggatga cccttttcgg aactaccgaa tggtttggtc ttaatatcgg cttttgggtg
     9901 gctatggcag ttgtattact gattgtcatt gtaatgaatg tagtgttttg gtccatgaaa
     9961 ccaaaaggag acgcaaaaga gtcttataaa taaaatgtca gtatgaatga agcaagggag
    10021 atgcacagtt ttttattgtt tgtttacgcg tctataacga aatcgtgaaa taatattaaa
    10081 aggtagattt tattatggaa tgtatgtctg tacaagaagt ttcgataaaa tgaggaaatc
    10141 tgtgaaagaa gaataaaatt agttatatgt gaatatgtat ccatagttgt tgaaatttta
    10201 gtgtgatgta gtatatgttg gatatctttt atactagcac cattttctaa taacatagta
    10261 gcagtgtatt taaaataaag tgtgtattct tctaaaaagt tgtgataatc aaaatatcaa
    10321 atatcactat acaaaggaag atgcacattt tttatcttta ataaaaacga aatattaaaa
    10381 aaatttattg aggaatgtga tgtaaaaaca actacagata ttcaggagaa aatagtacgt
    10441 cacttttata gaataatgtt tattataaaa aataatataa tgaaatattt atagatttta
    10501 aaaaatatac tctattatca aataatattg tcatctaatt ttatatatat aatacttttt
    10561 aagtagaata tttacaaata gaattataaa aaagatggtg aattattata atgttcacca
    10621 tctttcagga aatatcgagt ctggaaatat ctaaagtaga ctttatacat taatatcctt
    10681 tgatatttta aatccaaggt tataagcttt ttttaatatt ttagggttat tttcaattga
    10741 gtttttttca aatgctttag tagcaattat agttttataa tgatttaaac ccattccttt
    10801 gagatgctta cttgtatatc taaaatatct tttaaagaat attggtaaag gtgctgcttg
    10861 agtataaacc ataattattc ttttatttcc gaatctaggt atatgtttac ctttatctat
    10921 tgttgtaagt ggataaagtc tatctaataa taatttggtt tgagctgata cctgacatat
    10981 ataaactggt gaacctataa ttacatactc agcttttttt atatcttcat atacttgaat
    11041 catatcatct ttgagtgaac atgaatgatt ttttctacaa tataaacatc cctgacaacc
    11101 ttttatattc atatcattta agtaatactt ttttacatca accttgtttt tatttgctcc
    11161 tttaattatt tcattaatta ttgtatctac attaccattt tttcttgggc ttcctacaaa
    11221 tgctattatt ttagacattt tcaacactcc tatttaatat atatttttga gattaggaaa
    11281 tatttgctct aaatattttt caactttttc atgtcttcaa tttcaaaatt actataaaat
    11341 attgtatcta ttttatttat tgcattttca gttattttaa taacattatc tacttttata
    11401 gtgtttaata tgagtgtaat tcttttatta caatcacaca agtttttata tattcccctg
    11461 aacttctaat ctatctaaca tatttgttag tatagattta cttaattaaa tttctttgta
    11521 aaattcattt atagagtact ctttatatat acttattgaa aattaaatca ccttaacata
    11581 aatattccta tgattaatac tgtataatac taattatagg aatactagtc acttattcaa
    11641 attgtcaaac aaaatattta tctaatacct actatttaaa tttgcttaaa acagtttatt
    11701 caatatcttg aatatgctag ataagaaaaa aagtaaataa aaatgtagat aatacataat
    11761 tatattattc agttttaaca tatataaggg aacacaaaga atgcaacgca agagaaataa
    11821 aaaaattagc aagaacatat tctaagtttg aaagaaaata gtaatcatag tataaattat
    11881 atactttaat taatataaac agcaacataa gatatatcaa ttactctaaa gttaggttaa
    11941 atagtcttac tgaattatct ttgagaataa ttgtttcaat gtctagtaga aataccactt
    12001 gtttatagca tttgtagcta ctataatcga aatgtatttt tagtttttca cagaacaatt
    12061 ataatatagg ttagaggttt ttaggaatca cttctctttt tatataaaaa aggagataac
    12121 tcaagttttt attttgatac atccccattt ttataattta taataattct tgtctttatt
    12181 ttaaataaaa catagattta agttctatag atataggact attatcctca ttggtttcca
    12241 ttatatcaga catataattc caccattttt tgcagattgc tgtctctgaa cttttatccc
    12301 atagttttgc atctttaagt tctatatatc catataaata attggtttct tcatctagaa
    12361 aaatagaata gttactacca ccatattcat gaatcatatc tatcatctca gaccataatt
    12421 catcatgacg tttcttgtat tcattttcaa atcctttaaa aagcttcatc ttgaaacatt
    12481 tatgtactgt attcataaaa aaatacctcc taaattttaa atgtttactt ataaatttat
    12541 tttaaaacaa aaaaaggtat ttttaaatgt cataatttaa cattattact gtcaaaaatt
    12601 aatatgatac aattaaataa agaatattct taatctaatt taaaactgta ataaagtata
    12661 gctaattctg gagggatata aatgaaagat aaaacgctag aagatatact taaacaaaaa
    12721 acacaagcag aaaggtacat attagaatgt atagatacta ataatgtaaa aaagttagat
    12781 aataaaaaaa taatgtctat attgcatact attttatgtt gggaagaagt aaatggaact
    12841 atagtactaa cagataaaaa tatactttct ttagatgaag acattatggt atccaaacat
    12901 catagattta tagatatacc caaacataaa cataacttta tagaattaat ttatgtttat
    12961 agtggttcat gtactcaaat tatagatgga aaagaaacca tattaaaaga aggtgaaata
    13021 tgtctcttgg atatgaatgt agaacattca ataaatgcag taaaagaaaa tgacatcatt
    13081 attaattgta taatgaggag agaatatttt gatgcatctt tttttctaga gtttcgtcta
    13141 atgagttaat atataatttt ttagcaggag ctatacacaa aggaaaaaga tgtgataatt
    13201 attatatatt taatgctaaa aataatttta aattgaaacg actagtagct gaactaacta
    13261 aggaatattt gaataaatca atgtgttata aggaaataat ggattgttac attatattaa
    13321 tattttatga attgttagag atacataaaa ctaaaaatga aaaaacttta acatctgata
    13381 gtaaaattaa tatagataat attgcagaaa tgatgaaata tatagaggga aattatgcac
    13441 aaacaacttt aagggaggtt gctaaaaaat ttcattttca tccaaattat ttaagtactt
    13501 taataaaaaa gacaacaggt aaatcattca aggatatcat ccacgaacaa agacttaaaa
    13561 aagcaactac attattaaaa agtacagata tgagtataga agacatttct attgaagtgg
    13621 gatataataa ccaaaccttc ttctataaaa aatttaaaga gacatatagt ctgactccaa
    13681 aaaaatatcg tataaaatca aggattttat taaaggaggt atctcatgag acaatatatt
    13741 catgataagt tgagggaaat aacagaagaa gaaaaaaata tactagaagg aaattatata
    13801 atagataaaa gtatttatac tgataattct caatttataa tagatagcaa caagttattg
    13861 aatatagatg agttgataca tataagaaaa catacaagat ttacacaatt tccaaaacat
    13921 aaacataatt atatagaatt taattatgtt tatagaggaa aattggtaca aactatagat
    13981 gaatataaaa ttaatttaaa acaaggagaa ttaatatttt taaatcagca tgttattcat
    14041 gaaatagaag ctagtaacga agaagacatc ataattaatt ttataataaa ccagagtttt
    14101 ttgattatat tatatctttg ttggaaaatg ataatacaat aatgaacttt ttaatgacta
    14161 cactatatac taattataat cttggagaat atatgtactt ttatctaaat attttactca
    14221 aaaagaacta gcaaagaaac agttgcttaa ctatactgcg ttattaagtt ttgaaataga
    14281 cgcaaaggat gtaaatgata ttaaaaaatt tgtaaataaa ttagatttat ttaaaatagg
    14341 agtgagttgg ggaggtcatg aaagtttagt gtttgctcca aatatagcta tattaaatga
    14401 aatgacttta gaacaagcca aagcgttaaa tgtgtcacca tacacaatta gaataagttt
    14461 aggtcttgaa aatagtgaag atttaatcaa tgatttaaat gatgcgctat ccattttata
    14521 atataataag aatatatgga aaaacatatt aactaaatag tctatatgtt ttttcttttt
    14581 gtttgataag tcttgctagt atataataaa atttattatt attttattga cttgagatga
    14641 gtgttataag atatcacata acttattgta caaaaggtga tggagaaagt atcaaacttc
    14701 ttagtaaata aattttaaat aaatctgagt ctggaaatgt ttatatgtat tctttaaaca
    14761 aaactttatt ttttaagtga caagtaaaca tttattggta aagctatgac tttaaaaatt
    14821 atagcctttt ttgaattttt ctatttttgc aaaattatta tcactttcag aaaacttttt
    14881 gattttttga aatgcttgtt tattaactta gagttaaatt tttttattca aaataaatac
    14941 ttaaatgagc taaaatttga gtccaatttc taattttctg agtccatttt tcagaaatat
    15001 ccatagttgt tgaaaatttg ataatttact tagcaaagaa aaattattat caaagtacca
    15061 tacataaaca tatctattca gtgtatttat agagtatagg agtcagattt tagactacag
    15121 gattgatatc ataggggtta tagtttagac tgtaggatag atgtagacta taaggttaat
    15181 tagatataaa ggaggaggat gtatgaaaag agcatattat tcaagtagcg taaatgaatt
    15241 tataggtaaa gatattttta ctatatttgg tgagataaca tgtaatgatc aatttgaggc
    15301 taatgacctg caaaagaata catggaaaaa ggaaattgaa atattaaaaa gagagctctc
    15361 aactttcaaa gatggacact tattatttga gtatacaata ccacgaatag gcagtagaat
    15421 tgacaatgtg ttaatttaca aaggcattgt ttttctcctt gaatttaaag ttgatgaaga
    15481 tacttatcct aggtatgcaa ttgaacaagt tacagactat gcccttgacc ttaattgttt
    15541 tcacaaggaa agtcatgata aacttttagt accaatactt atttgtacaa aagctcctca
    15601 aagaaatcaa agaataagat tgatgaagga aaatatttta gaaacttatt gtagtaatga
    15661 gtttaatata ggaaagtata tagaggaggt ttgtactaca tataacaaaa gttcatttaa
    15721 tccagatgct tggatggact cattatacat gccaactcca acaattattg aagctgcaca
    15781 agctttatat atgggccata atgttgaaga tatttcaaga aatgatgcaa gtgctaaaaa
    15841 tttaaaccaa acaactaaag ctataaataa aattatagac tatagtaaga ttcataataa
    15901 aaaatcaata tgttttatca caggagttcc tggagcaggt aaaacgcttg ctggccttaa
    15961 tattgctatt gagcgacaaa aggtagatga aaatgaacat gcagtcttcc tttctggaaa
    16021 tggtccatta gttgatgttt tacaagaagc attagctcgt gatgatgtaa ccagaaatgg
    16081 aacacgaaaa actgaagcga tacgaaaagc aaaagaattt attcagataa tccatcattt
    16141 tagagatgaa gctatatctg ttgatacacc acctattgaa agagtcacaa tttttgatga
    16201 agcacaacgc gcatgggatg aacctaattt aactaacttt atgaaaagaa aaaaaggtgt
    16261 attagatttt aatatgtcag agcctgaatt tctcattagt attttaaatc gtcatatagg
    16321 atgggcgact attgtttgtt tgattggtgg aggccaagag ataaataaag gagagtcaga
    16381 tggaatatca ggttggtttg catcactaag aaataattat tctgattggg atgtttacat
    16441 atcaaataaa attattgatg aagaatattc taaaggtaat tcttttgatg aattaacaga
    16501 aggcgttaat tatagaataa ttgacgattt gcacctatct gtatcattac gttcttttag
    16561 aagtgaaaaa gtagcagcct ttgttaaagc cgttttagat gtagataaaa ttgctgcaaa
    16621 tgaattatat aatcagttta aaaaagatta tcctatatgt gttactaggg atttggaatt
    16681 agctaaaaag tgggtgagag ataaatcaaa aggttcacag cgttacggaa tgactgcaag
    16741 ttctggcgct aaacgacttc gaaagtatgg ggtttgggta caaagtaaag ttgatgcgaa
    16801 aaactggttt cttaatggta aggatgatgt acgagcttca tattttttag aagaaactgc
    16861 gacagagttt gatatccagg gcttagagct agattggaca attgtttgtt gggatggaaa
    16921 cttaagattt aagaatgagc aatttgaata ctataatttt aatggaacta aatggcagaa
    16981 tatcaataaa gaggataata tactatactt aaaaaatgca tatagagttc ttcttacaag
    17041 ggcaagacaa ggttttgtta tatttgttcc gacaggtgat gaaagtgata ttacaatgca
    17101 accaagtttt tataatggcg tatatgaata cttaaaagaa attggaatag aggaaattta
    17161 ggttggaagt aaggatagta ttaaatggat tatttgtaaa aaactatttt tctttataat
    17221 atatgagaaa agtaaaacct tgtaactaaa gaatacattc aagagataac aagaaaaaag
    17281 aagagaagca agctgagttg agaaatgaat attaataatt ttcaaccaat ctaaaattta
    17341 aatatggttt ttattataag caatatattt aattatattg tataaaata
//
LOCUS       DAFNNB010000075        17284 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0076, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000075 DAFNNB010000000
VERSION     DAFNNB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17284
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0076"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            5..496
                     /locus_tag="L3M60_002109"
     CDS             5..496
                     /locus_tag="L3M60_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3794 domain-containing protein"
                     /protein_id="HBN6226770.1"
                     /translation="MYENNKVIYNFERCEKLEKTLYLSQFNTYKNFKLTSKKTVINSF
                     ETYVCNMNIRSISLVDTIECISLEDEQLTGKKLILVGDIDTDVILDYVANKRNRNSSK
                     KNILKLKIPFSTFIQIPRKIENKDKINLKYLIQDISSSILDDNNLFISVTAIISYENS
                     SQI"
     gene            527..1474
                     /locus_tag="L3M60_002110"
     CDS             527..1474
                     /locus_tag="L3M60_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226771.1"
                     /translation="MRITKTTFNDVFSNDTNGSILKVEKYVDKKCASVFDILTYTIIV
                     TNVSRYKTGNIFFKDYISKYIEFVNNTVKVNGIIKRGLDPQQGFYIGRIDASCKKIIS
                     FKSVVLPNSAHRIIKNSANIYYYYKCNIDKFPTRISIESNRVYTNINKILFKQLNISN
                     TLKAPKNLKDILKVNANSKILDVKPIKNPTNKGNNLKSCNLIVFGSVDVEVDYSCKNR
                     SKFKTVNKSSDIQKDKHYSNDIQNNNKTISDCKRQISIGNNKVKKITKTFGFSCFICS
                     PVGIVYEDMKNINIKIEHTSINELNPDELFINTSLLLYY"
     gene            1764..2306
                     /locus_tag="L3M60_002111"
     CDS             1764..2306
                     /locus_tag="L3M60_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6226772.1"
                     /translation="MKICITVGHSILKGGKSTGASGIVDEYNYNKKLAPMLAEMLISQ
                     GNTVDIIICPERHFMSEREEFFYRVPKVNSGKYDLLVELHLNKADGTQCGTEVLYYGN
                     EGLEYAKRVSNRLGELFENRGAKKRENLYILRNTNPVAIQIESFFCDNVNDCNKANES
                     GYDYIARLITEGILNKDINM"
     gene            2635..4128
                     /locus_tag="L3M60_002112"
     CDS             2635..4128
                     /locus_tag="L3M60_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBN6226773.1"
                     /translation="MKYTFEKFKLNEKILKSLKSLEYNIPSRVQIEVIPKLLNGQNLV
                     VKSKTGSGKTASFAIPLCENINVEYNNIQALIVVPTRELALQVKDEISSIGRLRKVRC
                     SAIFGKQPIKEQIAELKQRVHIVVATPGRIIDHINKGTIKLEDIKYLIIDEADKMFNK
                     GFVEQMEKILLNLPKEKIVGLFSATIDEEIKYICEKYIPDYSVINIEENKSDTNQRTR
                     QIDDKIIKTNEREKYILLKELIYSENPKSVIIFCNTKEKVSKLYNKMSKEGFLIRELH
                     ADLSQERRIFVIKDFKKQKFNVLVSSDVASRGIHIDDISLVINYDVPQDKENYIHRIG
                     RTGRKRNSGKAITIVTEKDEKYIENIETYIGYKINELKDIEKSRITHGKILFEEYSKE
                     ILKEVSKRKKADKNSYKGKDIKLNTESEVVKLYLNAGKKKKIRVLDIVGAFSNIKGIT
                     NDDIGVIEVQDLCSYVDILNYKGDLILKKHKEIPIKKKMVKVKRDIR"
     gene            4367..5131
                     /locus_tag="L3M60_002113"
     CDS             4367..5131
                     /locus_tag="L3M60_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6226774.1"
                     /translation="MAYFNYNNKQCYYNEIGDGTPLLFLHGNTASSKMFNEIINFYKD
                     EYKVILIDFVGHGKSQMVDKFAADLWFDEAMQVICFLEVMNYKKVNIIGSSGGALVAL
                     NVALERPDLVNKVIADSFEGEVPLESFVQNVKIEREASKQDDGAKAFYIYNQGENWER
                     VVDNDTEAIFEHYKTIGKFFHKPLETMQPEVLLTGSREDDFVSLISNDFFENTFSSLL
                     EKIKNGKMYLFDKGGHPAILSNGLDFSNVAKKFLEE"
     gene            complement(5416..8595)
                     /locus_tag="L3M60_002114"
     CDS             complement(5416..8595)
                     /locus_tag="L3M60_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBN6226775.1"
                     /translation="MKIDMIYKLIRRNTEPSRWARGVKYYTNQLVDYIDIDIDDLQNN
                     LLTFRGGVNSEDNYSFYFNSITIDIKHFKIIENDCSCADCQSNGKNIFICKHIVAIVI
                     KSLKQIKNKKDVPPILIDILGADKDKLSSTNSKTSLDLSKQLLSLLDNTKRERVNLDV
                     LINMDSNKAIAEFKIGIDKMYILKNITEFAYSKLNGYELDFGKKFTYDPNIHYFADED
                     EKLVDIIEEYGTNLSYSFSSSHSKKFMEINASSLKQFLNAIKYKDFTLIFSKEEYHPK
                     ILNDTLPIRFDLKNIEDKIVLCSKDSLPVPLTKKNDVLLYDGNIYLLSHKKAHDYSKI
                     YQVLSKTKELKFDKEDSKDVLGLLVPRLKSISQEVHLDDNIKNNITKDFKSEFYFDMI
                     DSNICCDVKYIYDDEDKKFVLPNIQKETTIENKLTSSQFTKENNHYVFKGTDHDLFTF
                     LDLQLESLKEFGDIYYSDKFKLKKIYNASSIKASINQTNQNYLEFNFNISDINPKEYK
                     DILKAFQEKRTFYKLKDGNFIDLSERETKDFFELVENLDIMGTSKNNKIHKNKALYIN
                     DMIRSKNLKFIDGKKELDSICEKFRNFDSINLEIPANLNANLRDYQINGLNWFKVLDY
                     YKFGGILADEMGLGKTIQTIAFLLSLSNKKSLIVTPTSLIYNWKNEFEKFAPDIKVLL
                     IHGSKRDREKCFMELENFDVILTTYGTLRNDLEKYSEIKFDYCILDEAQNIKNPLALV
                     TASVKSINAENKFALTGTPMENNLLELWSIFDFIMPGYLYSKAKFQELFINKEDNVKN
                     LKKLIKPFILRRSKKQVMKELPDKIEKNFFVELNKEQKKIYSVYSKDIQDKIKDKNLK
                     KDKIVIFSYLTKLRQLCLDPSIVVKDYNKKSSKIETCLEILRDSINENHKILLFSQFT
                     SVLKNISKELDKYKIKYHYIDGKTNAKERLELVDEFNNSIDKKVFLISLKAGGTGLNL
                     TSADMVIHFDPWWNPSVENQASDRAHRFGQKNSVQVIKLIAKGTIEEKIIKLQESKKE
                     LINQFINGELSNEGVLKSLSDEEIIDLFN"
     gene            8876..11053
                     /locus_tag="L3M60_002115"
     CDS             8876..11053
                     /locus_tag="L3M60_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBN6226776.1"
                     /translation="MYNKCNNLYLLLGKENINMDSYRQLKAKIPCKETGIEIRRTLCD
                     ICSPGVHCGIDAYVKNGKVIKIEGTKEHPMNKGLLCTKGLCNREYIYREDRIKTPLKR
                     IGKRGEGKFKTISWDEAYREIAEKLNKVKSEYGAKHVAFFTGYGKWYRPFLQRFASSF
                     GSPNYGTESSSCSTSAQMAGNVATGRNFTMQDMENAKTYLGWGFNPYYSKFLNSVNLT
                     KLKEKGLKFIIVDPRITPTTQKLADLHLRIKPGTDGALALGMANLFIQRDLIDKEYIE
                     KHIHGFNEYKEYVKLFDLDTVSRITRVSKDNIVKAVEMLSQGSFCINESAAPIVHHQN
                     GFQNYRAMMSLSAITGNYDRIGGNIPKQDYFAGKIGGFQTLEKEFVKEVLPKNINERI
                     GGERFPLWNEMVTQFQAMDLPRQIEEKTPYEIRALFCHGINARMFPNSGRLFKALDSV
                     DFFVNIDLFMTDTSKYADILLPACTSFEREECKSYPGGYITYTKKVIDKLYDSKSDVE
                     ILSDLANVMNIDDDLLKAGYEASIRHMFKNTCVDVDKLKESDLPLKCKDFKPYIVGSY
                     TREGYDTSTSRFELKSTIIEKYKDFGLDALPTYRGFNDNIDDEYTYLLTSGARISNAL
                     HSRLHDIKLSRILKNEASCDISSLDAKELGVISGDYIKITTKIGSLDVKVNVTDRVLY
                     KNIHMYHGYREADINSIICDNFDPYSGFPAYRSCKCKIEKTNR"
     gene            11067..11489
                     /locus_tag="L3M60_002116"
     CDS             11067..11489
                     /locus_tag="L3M60_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6226777.1"
                     /translation="MKSLFKFNKELCTACGACLLACIDQNDNNIDNGELFRHVFTIEE
                     GESIEYFSLACIHCDEPKCLDVCPKNCFKKEEGFVVLDNQNCIGCKLCEKACEYGALI
                     YGMDGKANKCNGCVERYKCGMDSPCVMVCPTGALKFNK"
     gene            11897..12700
                     /locus_tag="L3M60_002117"
     CDS             11897..12700
                     /locus_tag="L3M60_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBN6226778.1"
                     /translation="MKKIKLIIMLALVSAFLITGCSSENNKNKEEQKTIRVATSGTYY
                     PFAFKEKDKLKGFEVDFWDEFAKRTNCKVEWVLTDFSGLFGLLEADKADVVSAQLTAT
                     PEREKKYDFSDAYNYSGTNIIVREDDTSIKSIDDLVGKKVGVGTGAVANEILKEKYPN
                     DEIKIVNYSSATLKGNYQDLEMGRLDAVVAQDVEALIAIKEQKLNLKLVEPPIQFGAC
                     SFALQKNEKGERWTKEINKVIEDMHKDGTLTKISKAWLGKDITKEPINE"
     gene            12693..13361
                     /locus_tag="L3M60_002118"
     CDS             12693..13361
                     /locus_tag="L3M60_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBN6226779.1"
                     /translation="MSSFNFEFCLNIFPFLIKALGITILISIIAFFISILLGVILAFF
                     TQTKSKILQYITRVYVSFFRGTPLLIQIFFFYFGFPMVFSFMKDCSPYLAIVICLGVN
                     SSAYMAEIIRASIESVDKGQQEACYAFGLSKYQSMVKIILPQAATSAVPPIVSCTLDI
                     IKGSSLGLTIGIQDLMGVAQVEAASSFKYFEIYMVTILIYWAVSIMLSNIQSKVEKRI
                     GKAY"
     gene            13596..14258
                     /locus_tag="L3M60_002119"
     CDS             13596..14258
                     /locus_tag="L3M60_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBN6226780.1"
                     /translation="MIFNMKYFLELFPIIMKSFNVTVILAISSLVFSLIIGTIVALSS
                     YYKVKVLNQICKVYVSIFRGTPLMPQLFFLYFGLAYMSDFVKNMDPVFATSIVLSLNM
                     GAYMSETIRASILSIDKGQIEAAYAMGLTNLQTMKRIVIPQAVRVALPSLFNNFIDLI
                     KGSSVAFVVGVNDIMGAAKSQGALSFKFFEVYGAVMVIYWCIITPLGWGQKKLEKKIS
                     NY"
     gene            14318..15115
                     /locus_tag="L3M60_002120"
     CDS             14318..15115
                     /locus_tag="L3M60_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6226781.1"
                     /translation="MIKRNIIKCLVAVLSLTVLVTGCSKKEEVKDEKVITVASSNSAD
                     PYSYVDNGEHKGFEVDMWKEIGKRSGYKIKMEPTGFSSIFGMLDSGKVDVAANFFGMS
                     KERLEKYDASIPYGSDSVCIAVKDGNNEINKLEDLKGKVVAVSEGSQGQQVAASVNKD
                     NLFEEKVYGDGTNGLQDLDLGRVSAWIDAELTVIGDAKKANMKVRVLDEKLSVTNIAY
                     FFKKNDEKSKTIKEDVNKAIEEMLADGTVKKISEKWFGMDVSADIQK"
     gene            15248..16423
                     /locus_tag="L3M60_002121"
     CDS             15248..16423
                     /locus_tag="L3M60_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6226782.1"
                     /translation="MIETKIKSIALKNREKIIKIRRQIHSNPELAFKEFKTSKLIKEE
                     LNKLNIEYIDVAGTGVLATIKGKNNGGKTILLRADMDALPIKEENDLEFKSINDNMHA
                     CGHDAHVSWLLGTAMILNDIKEELNGNVKLLFQPGEEKGGSDIVIKENVLEGVDALAT
                     GHCWPTIESGKIGIARNCAMAATNTFEITIIGKGGHGAEPHNCIDPIAVGNAVYSSIQ
                     QIVSRKINPVIPVVVSVCSFNSGVSKNIIPDVCTLQGTIRAISQEKVIEISKILENIV
                     RGVCKSNGADCKFEKSMGGDAVINDKDMIELGKKSACKILGYENVEMIDFPAMTGEDF
                     AIYMKERPGLFMYIGVGSEEKNINYRLHNNKFDIDEKCLSVASSLFSQLAVDYLCQL"
     gene            16532..17281
                     /locus_tag="L3M60_002122"
     CDS             16532..17281
                     /locus_tag="L3M60_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBN6226783.1"
                     /translation="MIKVENLHKSFKGTEVLKGINLEINQSEVVAILGPSGTGKSTLL
                     RCLNYLVEPTKGKIMIGDVKVDVERTTKKDIANLRKHTSMVFQNYNLFKNKTALENIM
                     EALIVVHKKGKQEAREISLKLLDKVGMLDRKDFYPSKLSGGQQQRIGIARAMAVNPNV
                     ILFDEPTSALDPELVGEVLSVIKSLAKEGITMLIVTHEINFAKEVADKIIFMDKGVIA
                     EEGTPDEIFNNPKNERTAKFLNIIKKEEVFS"
BASE COUNT         6812 a         1782 c         2804 g         5886 t
ORIGIN      
        1 gattatgtat gaaaataata aggtaatata caactttgaa agatgtgaaa aactagaaaa
       61 gactctttat ttatctcaat ttaatacata taaaaatttt aaattaactt ctaaaaaaac
      121 tgtaataaat tcatttgaga catatgtttg caatatgaat attcgcagta taagtcttgt
      181 tgatactata gaatgtatat cattagaaga tgaacaactg acaggaaaaa agttaatttt
      241 agttggtgat atagatactg atgtaatatt agattatgta gccaataaaa gaaatagaaa
      301 ttctagtaaa aaaaatatac ttaaattaaa aatacccttt agtacattta tacaaatacc
      361 tagaaagata gaaaataaag ataaaattaa tttaaagtat cttatacaag atatttcctc
      421 atctatttta gatgataata atttatttat tagtgttact gcaattataa gttatgaaaa
      481 tagctctcaa atatagtaat atttaaatta tagaaaggtg gatgcaatga gaataacgaa
      541 aactactttt aatgatgtat tttcaaatga tacaaatgga agtattttaa aagtagaaaa
      601 atatgttgat aaaaaatgtg catctgtatt tgatattttg acttatacta ttattgttac
      661 aaatgtaagt agatataaaa ctggaaatat atttttcaaa gattatattt ctaaatatat
      721 agagtttgta aataatactg taaaagttaa tggtattata aagcgtggat tagacccaca
      781 acaaggtttt tatataggga gaatagatgc aagttgtaaa aaaataatat cttttaaaag
      841 tgtagtttta ccaaattcgg cacataggat tataaagaat agtgccaata tatactatta
      901 ctacaagtgt aatatagata aattcccaac aagaatatca atagaaagta atagagttta
      961 cactaatata aataaaattt tatttaaaca gttgaatata agtaatactt taaaagctcc
     1021 taaaaatcta aaggatatat taaaagtaaa tgcaaatagt aaaatacttg atgttaaacc
     1081 tataaaaaat cctacaaaca aaggtaataa tttgaaatca tgtaacctta tagtttttgg
     1141 gagtgtagat gttgaagtag actatagttg taaaaataga agtaagttta aaacagtaaa
     1201 taaatctagt gatattcaga aagataagca ttatagcaat gatatacaaa ataataataa
     1261 aactataagc gattgtaaaa ggcaaataag tattggtaat aataaagtaa aaaaaataac
     1321 aaaaactttt ggattttcct gttttatttg ttcaccagtt ggaattgttt atgaagatat
     1381 gaaaaatata aatataaaaa ttgagcatac atcaataaat gaattaaatc cagatgaatt
     1441 atttataaac acatcgcttt tattatatta ttaggtattt taaatctaaa acttaaaatt
     1501 attaaacgag catttgatta aagagaagta tatattgata tatttatttt atgtgtaaat
     1561 gattaattaa ataattataa gtaataagta tagtataata tttaaaaaat atgtatttat
     1621 gataaaataa gacaaatatg ttaaaaaaat gataatcacg gagaactttt accttctcca
     1681 attaatatat tattattagt aataacttta agattatatt actattatat aaaaattggc
     1741 ttaaaagaag gtgaaaaaag taaatgaaaa tatgcataac agtaggtcat agtatactaa
     1801 aaggtggtaa aagtacaggt gcaagtggta tagttgatga atacaattat aacaaaaaat
     1861 tagctcctat gttagctgag atgcttataa gtcaaggtaa tacagtagat ataataattt
     1921 gtccagaaag acactttatg tcagaaagag aagaattttt ctatagagtt ccaaaagtaa
     1981 acagtggtaa atacgattta ttagtagagc tacatttaaa taaagctgat ggaacacaat
     2041 gtggtacaga agtgctttac tatggtaatg aaggattaga atatgctaaa agagtatcta
     2101 atagacttgg agaattattt gaaaatagag gagctaaaaa aagagaaaat ttatatatat
     2161 tgagaaatac aaatccagtt gcaatacaga ttgaaagttt tttctgtgat aatgtgaatg
     2221 attgtaacaa agcaaatgaa tcaggttatg attatatagc acgattgatt acagaaggta
     2281 tattgaataa agatataaat atgtgagatt atggtgattt aggtattgat taataccttt
     2341 aatattagat ataaataaaa tacaatatat tagtataatt taaacttgta aaagatatat
     2401 tagtagttta gttgttgaca agggatacct ccttgaaaat attgatttat cagtattttc
     2461 aaggaggtat ttaaatataa aaatttaatt tatttactac taaattatag ctttacatag
     2521 tatatatttt tatcttcact ctaataaata atataaaaat agtagaacct tgtattataa
     2581 taatataatg tagaaaaata catagataag ataaatataa taaggtggta atatatgaaa
     2641 tatacatttg aaaaatttaa gttaaatgaa aagatactta agtcattgaa gagtttagaa
     2701 tataatatac cttctagagt acaaatagaa gttattccaa aattattaaa tggtcaaaac
     2761 ttagtagtga agtcaaaaac aggaagtgga aagacagcaa gttttgcaat acctttatgc
     2821 gagaatataa atgtagagta caataatatt caagcactta tagtagttcc aacaagagaa
     2881 ttggctttgc aagtaaaaga tgagatatcg agtataggaa gactaaggaa agtgagatgt
     2941 agtgctatat ttggcaaaca acctataaaa gaacaaatag ctgaattaaa gcaaagggtt
     3001 catattgttg tagctactcc aggtagaata attgaccata taaataaagg tactattaag
     3061 ttggaggata taaagtattt aataatagat gaggccgata aaatgtttaa taaaggtttt
     3121 gttgaacaaa tggaaaaaat attattaaat ttacctaaag agaaaatagt aggtcttttt
     3181 tcagctacaa tagatgaaga gataaaatat atatgtgaaa aatatatacc agattacagt
     3241 gttataaata tagaggaaaa taagagtgat actaatcaaa gaacaagaca aatagatgac
     3301 aaaataatca agacgaatga aagagagaag tatatccttt taaaagaatt aatatacagc
     3361 gaaaatccaa agagtgtgat aatattttgt aatactaaag agaaagtatc taaattatat
     3421 aataagatga gtaaagaagg ttttttaata agagagttac atgcagattt atcgcaagaa
     3481 agaagaattt ttgtgattaa ggattttaaa aaacaaaagt ttaatgtatt agttagtagt
     3541 gatgtagcgt ctagaggaat acatattgat gatatttctt tagtaataaa ttatgatgtg
     3601 ccacaagata aagaaaacta tatacataga ataggtagaa ctggtaggaa gagaaatagt
     3661 ggaaaggcaa ttactatagt aactgaaaag gatgaaaaat atatagagaa tatagaaacg
     3721 tatataggat acaaaataaa tgaattaaaa gatatagaaa aaagcagaat tacgcatggc
     3781 aaaattttat ttgaagaata ttcaaaagaa atcctaaaag aagtgtcaaa gagaaagaaa
     3841 gccgataaaa attcttacaa aggtaaagat ataaaactaa atacagaaag tgaagttgtt
     3901 aaattatatt taaatgctgg taagaagaag aaaataagag tcttagatat cgttggtgca
     3961 tttagtaata taaaaggtat tactaatgat gatataggtg taattgaagt tcaagattta
     4021 tgttcatatg tagatatatt aaattataag ggagatttaa tattaaaaaa acataaggag
     4081 atacctataa agaaaaaaat ggttaaggtg aaaagagata tacgataatt tagttattac
     4141 tgctctttct gatgaagaaa tcgtaggttt aagtaaaagt atcaatgttg atgaattgct
     4201 ctgttatcta aatgaagttg caaaaaaaca atagaagttg ttaagtaaaa gaatgttgat
     4261 tttacctata ttctaggcta tactattttt aagtgattat gctaatagtc attttaaata
     4321 taagtaataa atatatgttt atagaaacat aaaaggaggg aaatatatgg catattttaa
     4381 ctataataac aaacaatgct actataatga aattggagat ggtacaccat tacttttttt
     4441 acatggaaat acagcctctt ctaagatgtt taatgagata atcaattttt ataaagatga
     4501 atataaagta atattaattg attttgtagg tcatggaaaa tctcaaatgg tcgataagtt
     4561 tgccgctgac ttatggtttg atgaggctat gcaagtgata tgttttttag aagtgatgaa
     4621 ctataaaaaa gtaaacataa ttggtagtag tggaggagct cttgttgcat taaatgttgc
     4681 acttgaaaga cctgatttag tgaataaggt aattgcggat agttttgaag gagaagttcc
     4741 tctagagtca tttgtacaaa atgtgaaaat agagcgtgaa gcatcaaagc aagatgatgg
     4801 tgcaaaagct ttttatattt ataatcaagg agaaaattgg gaaagagttg tagataatga
     4861 tacagaagct atttttgaac attataaaac tataggaaaa ttttttcata aacctctaga
     4921 aactatgcaa ccagaggtgc ttttaacagg aagtagagaa gatgattttg tatctttaat
     4981 atctaatgat ttttttgaaa atactttttc atcacttctt gaaaagatta aaaatggtaa
     5041 aatgtatttg tttgataaag gtggtcatcc tgcaatttta tcgaatggat tagatttttc
     5101 aaatgtagca aagaaatttt tagaagaatg atttaaccca aatttataat aatgaaaact
     5161 ataaaataga ttgtaaaact tacaaaagta agtgtacata tactttattc ttatgatgaa
     5221 atagaattgt taaaacaaaa tattctaata gttaagttat tacatttgta tattcaatag
     5281 gtgtagaaag agatattatt ggattataaa gttgggatta aataataata tgaagtttta
     5341 tttattcaag tattgtagct taactatata aatcttagaa taaatttata tagttaagtt
     5401 gtaggattaa ataatttagt taaataagtc tattatctcc tcatcagata gactttttaa
     5461 tacaccttca ttagaaagtt ctccatttat aaattgattt ataagttctt ttttactttc
     5521 ttgtaatttt attatctttt cttctatagt tcctttagca atcaatttta taacttgtac
     5581 tgaatttttt tgaccaaatc tatgagctct atcactagct tgattttcta ctgatggatt
     5641 ccaccatggg tcaaagtgaa ttaccatgtc agcagatgta agatttaaac ccgttccacc
     5701 tgcttttaaa gatattaaaa aaactttttt atctatactg ttattgaact catctacaag
     5761 ttcaagtctt tctttagcat tagttttacc atctatataa tggtatttaa ttttatactt
     5821 atcaagttcc ttacttatgt tcttaagcac agaggtaaat tgtgagaaca acaatatttt
     5881 gtggttttcg tttatactat ctctaagtat ttctaaacat gtttcaattt ttgaactttt
     5941 cttattgtaa tcttttacta ctatacttgg gtctaaacaa agttgtctta gtttagttaa
     6001 ataagaaaat ataactattt tatctttctt tagattttta tcttttattt tatcttgtat
     6061 atcctttgaa tatacactat atatcttttt ctgctcttta ttcaattcaa caaaaaagtt
     6121 tttctcaatc ttatctggaa gttctttcat aacttgtttt ttacttcttc taagaataaa
     6181 aggttttatc aattttttca gatttttaac attatcttct ttatttataa ataattcctg
     6241 aaactttgct ttactatata gatagccagg cattataaaa tcaaatatag accataattc
     6301 taggagatta ttttccatag gtgtaccagt gagtgcaaat ttattttctg catttatact
     6361 ttttacagaa gcagttacta aagctaatgg attttttata ttttgagctt catctaatat
     6421 gcaataatca aattttattt cactatattt ttctaaatca tttctaagag taccatatgt
     6481 tgtaagtatt acatcaaaat tttctaattc cataaaacat ttttctctat ctcttttact
     6541 tccatgtatc aataaaactt ttatatctgg agcaaatttt tcaaattcat tcttccaatt
     6601 ataaatgagt gatgttggtg taacaataag acttttttta ttggataatg ataataaaaa
     6661 tgcaatagtc tgtattgttt taccaagacc catttcatca gctaatatac caccaaattt
     6721 ataataatct aaaactttaa accagttgag accatttatt tgatagtcac gaagatttgc
     6781 atttaaattt gcgggaattt ctaaatttat actatcaaag tttctaaatt tttcacatat
     6841 actgtctagt tcttttttac catctataaa cttcaaattt ttactacgta tcatatcatt
     6901 tatatataga gctttatttt tatgaatttt attatttttt gatgtaccca ttatatctag
     6961 attttcaact aattcaaaaa aatcttttgt ttctctttct gataaatcta taaagtttcc
     7021 atctttaagt ttataaaaag tgcgtttttc ttgaaaagct tttaaaatgt ctttgtactc
     7081 ttttggattt atatcagata tattaaaatt aaattctaga taattctggt tagtctgatt
     7141 gattgaagct ttaattgatg aagcgttata tatttttttc aatttaaact tatcagaata
     7201 atatatatct ccaaattcct ttagagattc taattgtaaa tctaaaaatg taaataggtc
     7261 gtggtcagtt cctttaaaca cataatgatt attttctttt gtaaattgtg aagaagtcag
     7321 tttattttca atggtagttt ctttttgtat gttgggaagt acaaattttt tatcttcatc
     7381 atcatatata tactttacat cacaacatat attgctatca atcatatcaa aatagaactc
     7441 agatttaaaa tcttttgtta tattattttt tatgttatca tctaaatgaa cttcttgaga
     7501 tatagatttt aatctaggaa ctagtaatcc taaaacatcc ttagaatcct ctttatcaaa
     7561 ttttagctct ttagtctttg ataatacttg atatattttt gaataatcat gagctttttt
     7621 atgggataac aaatagatat tgccatcata taaaagtaca tcattttttt tagtaagtgg
     7681 aacaggtagt gaatctttag aacataagac tattttatct tctatatttt ttaagtcaaa
     7741 ccttataggt agagtatcat ttaatatttt tgggtgatat tcttctttag aaaaaataag
     7801 ggtaaaatct ttgtacttaa tagcatttaa aaattgtttt aaagagcttg catttatctc
     7861 cataaatttt tttgagtgtg atgaagaaaa actgtatgaa agatttgtcc catattcttc
     7921 tataatatct actaactttt catcttcatc agcaaaataa tgaatgttgg gatcataagt
     7981 aaattttttc ccaaaatcta attcatatcc attaagtttt gaatatgcaa attcagtaat
     8041 gttttttaaa atgtacattt tatcaattcc tattttaaat tcagcaattg ctttatttga
     8101 atccatattt atcaatacat ctaaattaac cctttctctt ttagtattat ctaacaaaga
     8161 aagtaattgc ttgcttaaat ctaagctggt tttagaattt gtactagaga gcttatcctt
     8221 atctgcacct aatatatcta ttagaatagg aggaacatct tttttatttt ttatttgttt
     8281 taagcttttt atcacaatag caactatatg tttacatata aaaatgtttt taccatttga
     8341 ttgacagtca gcacaactgc aatcattttc aattatctta aagtgcttta tatctatagt
     8401 tattgagtta aaataaaagc tataattatc ttcagaattg actccaccac gaaatgtcag
     8461 cagattattt tgtaggtcat caatatctat gtctatataa tcaactagtt gattggtgta
     8521 atattttaca cctcttgccc atcttgatgg ttctgtattt cttcttatta atttatatat
     8581 catatctatt ttcatattat tcaccttacc tcactgtata tattataatc taaatgcatt
     8641 caatttacac tctaaattta agactaaaat tagggcagat attgatagct tttatctatt
     8701 gcaagatatg tataaataac aatttactat tagatttttg ttgaattatc ttataaacta
     8761 tgagtgaaaa ataattatat aaatacatta ttagtaaata taaaatttta ttattaaaac
     8821 tattctttga aattacaata tataaaatat aaattatgat atttgaataa aaagaatgta
     8881 taataagtgt aataatttat atttattatt aggaaaggaa aatattaata tggatagtta
     8941 tagacaactt aaagctaaaa taccatgtaa ggaaacaggt atagaaataa ggagaacttt
     9001 atgtgatatt tgttccccag gagtacattg tggaattgat gcatacgtta aaaatggtaa
     9061 agtaataaaa atagaaggta caaaagaaca tccaatgaat aaaggattgt tatgtacaaa
     9121 aggtctttgc aatagagaat atatctatag agaagataga ataaaaacac ctctaaaaag
     9181 aattggaaaa agaggtgaag gaaaatttaa gacaatatca tgggatgaag cttatagaga
     9241 aatagcagaa aaattaaata aagtaaaatc tgagtatgga gcaaagcatg tagctttttt
     9301 tacaggttat ggaaagtggt atagaccgtt tttacaaagg tttgcatctt catttggttc
     9361 tcctaattat ggtacagaat ctagttcatg ttcaacatca gcacagatgg cgggaaatgt
     9421 tgctactgga agaaacttta caatgcaaga tatggagaat gcaaaaactt acttaggatg
     9481 gggatttaat ccatactact ctaaattttt aaatagtgta aatctaacaa aattaaaaga
     9541 aaaaggtcta aaatttataa ttgtagaccc taggataaca ccaacaaccc aaaaactagc
     9601 ggatttacat ttaagaatta aacctggaac tgatggggct ttagctcttg gaatggcgaa
     9661 tctatttata caaagagatt taatagataa agaatacata gaaaagcata tacatggatt
     9721 taatgaatat aaagaatatg taaaactatt tgatttagat actgtaagta gaattacaag
     9781 agtttctaaa gacaatatag taaaagctgt agaaatgttg tcacaaggtt cattttgtat
     9841 aaatgaaagt gcagctccaa tagttcatca tcaaaatgga ttccaaaact atagagcaat
     9901 gatgtcttta tctgctataa caggcaatta tgatagaata ggtggaaaca taccaaaaca
     9961 agattacttt gctggtaaga taggtggatt ccaaacacta gaaaaagagt ttgtaaaaga
    10021 agttctacct aaaaatataa atgaaagaat tggtggagaa agattccctc tatggaatga
    10081 aatggtaact caatttcaag ctatggattt acctaggcaa atagaagaaa agacaccata
    10141 tgaaataaga gctttgtttt gtcatgggat aaatgctaga atgtttccta attcagggag
    10201 actttttaaa gcattggata gtgtagattt ttttgtgaat atagatttat ttatgacaga
    10261 tacatcaaaa tatgcagata tacttttacc tgcatgtaca tcattcgaga gagaagaatg
    10321 taagagctat ccaggagggt atattacata taccaaaaaa gttattgata aattatacga
    10381 ttcaaaatca gatgtagaaa tactttcaga tttagcaaat gttatgaata tagatgatga
    10441 tttattaaaa gcaggttatg aagcaagtat aagacatatg tttaaaaata cgtgtgtaga
    10501 tgtagataaa cttaaagaaa gtgatttacc attaaaatgt aaagatttta agccatatat
    10561 agtaggttca tatacaagag aagggtatga tactagtact tctaggtttg aattaaaatc
    10621 tacaattatt gaaaaatata aagattttgg gctggatgca ctaccaacat atagaggatt
    10681 taatgataat atagatgatg aatatactta tttattgaca tcaggagcaa gaatttcaaa
    10741 tgcactacat tcaagacttc atgacatcaa actttcaaga atattaaaaa atgaagctag
    10801 ttgtgatatt tctagcttag atgcaaaaga attgggtgtt atcagtggag attatataaa
    10861 aataactact aagataggaa gtttggatgt aaaagtcaat gttactgata gagtcttata
    10921 taagaatata catatgtatc atggatatag agaagctgat ataaatagta ttatttgtga
    10981 taattttgac ccttatagtg gattccctgc gtatagaagt tgcaagtgta agattgaaaa
    11041 aacaaataga taaatggaga gatataatga aaagtttatt taaatttaat aaagaattat
    11101 gtacagcatg tggagcatgt ttgcttgcat gtatagacca aaatgataat aatatagata
    11161 atggtgaact ttttagacat gtatttacaa tagaggaagg tgaaagtata gaatattttt
    11221 cattagcatg tatacattgt gatgagccaa aatgtttaga tgtatgtcct aaaaattgtt
    11281 ttaaaaaaga agaaggattc gttgtattag ataatcaaaa ctgtataggg tgtaaacttt
    11341 gtgaaaaagc ttgtgaatat ggagccctaa tttatggaat ggatggtaag gcaaataagt
    11401 gtaatggttg tgtcgagaga tataagtgtg gtatggattc accttgtgta atggtatgtc
    11461 ctacaggagc attgaaattt aataaataga ttaaatatat agagtgactt atacttagat
    11521 ataagatatt tttacaaaaa gctatattga gtttgttata ataaaaactc aatatagctt
    11581 tttgtatgga tatagttgga tactataata tgattcttag taatagtttt ttgctattgg
    11641 acaaagactg taaaacatat taaacttatg ttgttcaaag tcgaagtata atataataac
    11701 tagaaaattg atttatagtg ttataaaagc aaatttagga aggtataaaa tctgaagtaa
    11761 ttctttacta cagtatcaat acttggagtt ctgttatggc agagtcatta aacaattata
    11821 atggtacaag tgattcaatt gctggaaatg tattatataa aaatttaaaa aaagattaaa
    11881 aaggagagaa taaattatga aaaaaattaa attaattatt atgcttgcct tggtatcagc
    11941 atttttaatt acaggatgct catcagaaaa taataaaaat aaagaagaac aaaaaactat
    12001 aagagttgca acatcaggaa catattaccc ttttgctttt aaagagaaag ataaacttaa
    12061 aggatttgaa gtagattttt gggatgaatt tgctaaacga actaattgta aagttgaatg
    12121 ggtattaact gattttagtg gattatttgg attattagaa gctgataaag cagatgttgt
    12181 atctgctcaa cttactgcaa cacctgaaag agagaagaaa tatgattttt cagatgctta
    12241 taactatagt ggaacaaata ttattgttag agaagatgat actagtataa aatcaataga
    12301 tgatttagtt ggaaagaaag taggtgtggg tactggagca gtagcaaatg aaatactaaa
    12361 agaaaagtac ccaaatgatg aaataaaaat agtaaattat tcaagtgcaa ctcttaaagg
    12421 aaattatcaa gacctagaaa tgggaagatt agatgcagta gttgcgcagg atgttgaggc
    12481 tttaatagct ataaaagaac aaaaattaaa tcttaaattg gtagaacctc caattcagtt
    12541 cggagcctgt tcctttgcgc ttcaaaaaaa tgaaaagggt gaaagatgga caaaagaaat
    12601 aaacaaagta attgaagata tgcataaaga tgggactttg actaaaatat ctaaagcctg
    12661 gctaggaaaa gatattacta aggagcctat taatgagtag ttttaatttt gaattttgtc
    12721 taaatatatt tccatttttg attaaagcat tgggaataac aatactaata tctattattg
    12781 cattttttat atcaatatta ttaggtgtaa tacttgcatt tttcacacag acaaaatcaa
    12841 aaattttgca atacataact agagtgtacg tttcattttt tagaggaaca ccacttttaa
    12901 ttcaaatatt tttcttttac tttggatttc caatggtatt ttcttttatg aaagattgtt
    12961 ccccttattt agcaatagta atttgtttag gtgtaaattc atctgcatat atggctgaaa
    13021 ttatacgagc atctattgag agtgttgata aaggacaaca ggaagcatgt tatgcttttg
    13081 ggttaagtaa atatcaatca atggttaaaa taattctacc tcaagcagct acatctgcag
    13141 tacctcctat tgtaagttgt acactagata taattaaagg ttcttcttta ggtctaacta
    13201 taggaataca agatttaatg ggagtagctc aagtagaggc tgcttcaagt tttaaatatt
    13261 ttgagatata tatggtaacg atactaattt actgggcagt atctataatg ttgagtaata
    13321 tacaaagcaa agttgaaaaa agaataggaa aagcatatta gatttcaata tttaaaaatg
    13381 ggatgatata gatatattat cacttgtaaa taaattttta attttagaaa aacaaattta
    13441 gtaattctaa atattgagaa aatataatac aaaatgaact ttataatcaa gttgaaaaat
    13501 ggttttttct attgaaaaat aaataaaatt ctattgtaat ttaatatgtg acaaaagttt
    13561 gtaaaaaaca acattaagct ggggggactt attttatgat ttttaatatg aaatattttt
    13621 tagaattatt tccaataata atgaaatcgt tcaatgtaac tgtgattttg gcaataagtt
    13681 cattagtatt ttcattaatt ataggtacaa tagtagcatt atcatcttat tacaaagtta
    13741 aagtattgaa tcaaatatgt aaagtatatg tatctatctt tagaggtaca cctcttatgc
    13801 cacagttatt ctttttatat tttggattag catatatgag cgattttgtt aaaaatatgg
    13861 acccagtatt tgcaacatct atagtgctta gtttaaatat gggagcttat atgtctgaaa
    13921 cgataagggc ttctatactt tcaattgata aaggtcagat agaagcagca tatgcaatgg
    13981 gattaacaaa tttacaaaca atgaaaagga tagtgatacc acaggctgta agagttgcac
    14041 tgccatcatt attcaataat tttatagatt taattaaagg gtcttctgta gcttttgttg
    14101 ttggagtaaa tgacataatg ggtgctgcta aatcacaagg agcgttgagt tttaagttct
    14161 ttgaagtgta tggagcagtt atggtaattt actggtgtat aataactcct ttagggtggg
    14221 ggcaaaaaaa attagaaaag aaaataagca attattaagt tattaagaaa atacataaaa
    14281 gtatttttaa ataaaaaatg gtgtgaggag aagagatatg ataaaaagaa atattataaa
    14341 atgtttagtg gcagttttat cgttaacagt tttagttact ggttgttcaa aaaaagagga
    14401 agtaaaggat gaaaaagtta taactgtagc ttcatctaat tcagctgacc catattctta
    14461 tgttgataat ggtgagcata agggttttga agttgacatg tggaaagaga ttggtaaaag
    14521 aagtggatat aagataaaaa tggaaccaac aggttttagt tctatatttg ggatgttaga
    14581 ttctggtaaa gttgatgtgg cagccaactt ctttggaatg tctaaagaaa gattagaaaa
    14641 atacgatgct tctatacctt atggttctga ttctgtatgt atagctgtaa aagatggaaa
    14701 caacgaaatt aataaacttg aagatttaaa aggaaaagtt gtagcagttt cagaaggttc
    14761 tcaagggcaa caggttgcag ctagtgttaa taaagataat ttatttgaag aaaaggttta
    14821 cggtgatgga actaatggtc ttcaagattt agatttagga agagttagtg catggattga
    14881 cgcagagctt actgttatag gagacgctaa aaaagctaat atgaaggtaa gagtattaga
    14941 tgaaaaatta tcagttacta atatagcata cttctttaag aaaaatgatg aaaaatctaa
    15001 aactataaaa gaagatgtaa acaaagcaat agaagaaatg cttgcagatg gaacagtgaa
    15061 aaaaatatct gaaaaatggt ttggaatgga tgtaagtgct gatatacaaa aataaatact
    15121 ttaaaattaa aatataaaac tgtgataact atagataagt atactaaatt tatctatagt
    15181 tatctgtgta ttacaatgta tttagattag tctttataaa ttaaaatgag ttatggaggt
    15241 cttgcaaatg attgaaacta aaataaaatc cattgcacta aaaaatagag aaaaaattat
    15301 aaaaataaga cgacagatac acagtaatcc agagttagca tttaaagaat ttaaaacatc
    15361 caaacttata aaagaagagc taaataaact taatatagaa tacatagatg ttgctggcac
    15421 aggagtatta gctactataa aaggaaaaaa taatggtggg aaaactatat tactaagagc
    15481 ggatatggat gcccttccaa tcaaagagga aaatgattta gaatttaaat caataaatga
    15541 taatatgcat gcttgtgggc atgatgcaca tgtttcatgg ttgttaggaa cagcaatgat
    15601 tttaaatgac ataaaagaag aattgaatgg aaatgtaaaa ttactatttc aacctggcga
    15661 agaaaaaggt ggttctgata ttgtaattaa agaaaatgta cttgaaggtg tggatgcatt
    15721 agcgactggt cattgttggc caactattga atcaggaaaa attggaatcg ctagaaattg
    15781 tgctatggca gcgactaata cttttgaaat aacaataatt ggcaagggtg ggcatggagc
    15841 agagcctcat aattgtatag acccaattgc agttggaaat gcagtatatt catccattca
    15901 acagatagtt tcaagaaaaa ttaacccagt tatacctgta gtagtgtctg tttgctcttt
    15961 taattctggt gtttctaaaa atataatacc agatgtatgc acacttcaag gaaccattag
    16021 agcgataagt caagaaaaag taattgaaat aagtaaaatt ctagaaaata tagtaagagg
    16081 tgtctgtaaa tcaaatggag cagattgtaa gtttgaaaag agcatgggag gggatgctgt
    16141 tataaatgat aaagatatga ttgagttggg aaaaaagagc gcttgtaaaa tacttggata
    16201 tgaaaatgtt gaaatgattg attttcctgc aatgacgggt gaggactttg ctatttatat
    16261 gaaagaaaga cctggtttgt ttatgtatat aggcgtagga agtgaagaaa aaaatataaa
    16321 ttacagattg cataataaca agtttgatat agatgaaaaa tgtcttagcg tagcatcttc
    16381 tttattctca caacttgcag tagattattt atgtcaacta tagagtacat tcataaaaat
    16441 atatttagcc tatttttaaa tttaatgcta gtatattaat tttaatgtag aaaatattat
    16501 ttattatatt tatttaaaaa ggtggataac aatgataaaa gtagaaaatt tacataaaag
    16561 ttttaaaggg acagaagttc ttaaaggcat aaatctagaa ataaatcaaa gtgaagtagt
    16621 agctatactt ggcccatctg gaacaggtaa atctacattg cttagatgtt taaattatct
    16681 agttgaacct acaaaaggaa aaataatgat tggggatgta aaggtagatg tagaaaggac
    16741 aacgaaaaaa gatatagcta atttaagaaa acatacatct atggtgtttc aaaattataa
    16801 cttatttaaa aataaaacag ctttagaaaa tataatggaa gcattaatag tagtacataa
    16861 aaagggtaag caagaagcta gagaaatatc tttaaaacta ttagataaag taggtatgtt
    16921 ggataggaaa gatttttatc catcaaaatt gtctggtggg cagcaacaaa ggataggtat
    16981 agcaagagcg atggcagtaa atccaaatgt aatattattt gatgagccaa catctgcact
    17041 tgacccagaa cttgttggag aagttttaag cgttataaaa tcgcttgcaa aagaaggtat
    17101 aacgatgctt attgtaactc atgaaataaa ttttgcaaaa gaagttgctg ataaaattat
    17161 atttatggat aagggcgtaa tagctgaaga aggaactcct gatgaaatat ttaataatcc
    17221 taaaaatgaa agaactgcaa agtttttaaa tatcattaaa aaagaagaag tatttagtta
    17281 aaag
//
LOCUS       DAFNNB010000076        17232 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0077, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000076 DAFNNB010000000
VERSION     DAFNNB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17232
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0077"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(80..1132)
                     /locus_tag="L3M60_002123"
     CDS             complement(80..1132)
                     /locus_tag="L3M60_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226784.1"
                     /translation="MNVINVFNINLKRNINKRIVFLVTLLFPIIIVILGVLANYISKP
                     FFNIGILDEYKNERVLKESEKRTYENTIMALENTQGINTKIADFKTIKTDIITGKYSA
                     VIYFNKNGFKLYSIKNKNTNDTLKYLIETYKKDPVPVDISKLQENTLGVLQRIIAFIV
                     LFLMITSTVTASMIIKDKNSGTFTRVLYSPQNVRGYVLGNMLYNFTITYFQYIVSISI
                     IKLFNIDIGMDYIDLILMGIWVVALATSFGTFVSSLFNREMHANLFSTCISLVLSLIG
                     GSFISVEKMPEMLQKISIISPVRWFISFVTYIEERDGSFFNMNYICILTSIIIVLALL
                     AMKFTQRSKYSSNSNY"
     gene            complement(1132..1872)
                     /locus_tag="L3M60_002124"
     CDS             complement(1132..1872)
                     /locus_tag="L3M60_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226785.1"
                     /translation="MRCFLPLFKLGIKRRSKDFFILFYNIVFPVIIIMLLGYLTSKSY
                     GEEFTSYNYYTIVTIPFCVLMGIISVSYAGQDEKISNTSYRYMISPISIVDLVISKLF
                     SCTAVLSICNLVTLVVLKCIFRMSFGGKFLAVLFFLMCESIVVVSMGLFLGLVCKNLD
                     MLRNIINIPIVIFGFLGGVFFPVSSLNPVISLIINISPLTWINRGIVACIYDNNLQLL
                     FNISCIFVVVSIFMTILTIKFFKKGVFI"
     gene            complement(1877..2635)
                     /locus_tag="L3M60_002125"
     CDS             complement(1877..2635)
                     /locus_tag="L3M60_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226786.1"
                     /translation="MSNILLEIKNLSKKYNNKEVVSIESFKLLEGDILGFIGANGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKDALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILEAIKCINTNGTTVIYTSHYMEEIETLCNRIALIDKGIIIEDL
                     DKSLYKNKYLEFGCKTLEDIFLYLTGTDLRDLEE"
     gene            complement(2737..3459)
                     /locus_tag="L3M60_002126"
     CDS             complement(2737..3459)
                     /locus_tag="L3M60_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6226787.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNDIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            complement(3560..4165)
                     /locus_tag="L3M60_002127"
     CDS             complement(3560..4165)
                     /locus_tag="L3M60_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6226788.1"
                     /translation="MKIKENVLRLDSTRGSNCFIVFDKEITLIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHMSNIKLLKEKTGAKVWAHTEDIPFIIGEKDRPGFKK
                     FIGKTISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVSYPNPWNWDSKKMMKSIRELDNLEYEWICMSHGTPCIK"
     gene            4345..4983
                     /locus_tag="L3M60_002128"
     CDS             4345..4983
                     /locus_tag="L3M60_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6226789.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFSLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSKKSDTEMLFKEKFFSFNKFMFDEIQEMFEIGKNDGSIRYDI
                     EISTLMYSSIFTLTGFFNLLSVTRKSYLNNFNIDEEKFIETTLAILIDSIKA"
     gene            5099..5761
                     /locus_tag="L3M60_002129"
     CDS             5099..5761
                     /locus_tag="L3M60_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226790.1"
                     /translation="MAKILLLEDDDTITFGIKTSLENKNHIVCCYNTIAEAKKNFDKT
                     TDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRAHSNTNNTITCGNISLNKVEVKAYLDSVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            5761..6942
                     /locus_tag="L3M60_002130"
     CDS             5761..6942
                     /locus_tag="L3M60_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6226791.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKVL
                     DASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKGTFWSKNFFYFLVVSFGI
                     ISTMAIVVYIIKYRTNKQKLSRINMLTRYLEEVNLGKESVLLRYEDEFSYLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TKQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     KGSPSIEFEIDINWTLEALLNILKNCSEHIEAGGHIIAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(7119..9779)
                     /locus_tag="L3M60_002131"
     CDS             complement(7119..9779)
                     /locus_tag="L3M60_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226792.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     VYRNATSEYWNHMPEKDSIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPVAPKSKGESFKKISKTTLTIVGKVDVATSSVVPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SNISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNKLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISTLIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAEAVYGSDNYKDQRDIEVYLENSEPVE
                     SDFIEKLDNTEGVKNSLYYQKMSATTWLTEKDASKELSKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDESFAEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLNLSIGDNIKLNEKIYDEDKGDYTYNMKVGAITSTLPKIIDTSSRYTLMQIVPIS
                     MVQEISKNYAEVGRSRSNRLIGLFKVDNREDIPRVRGKLESICNKDYGSGDYSIEDVV
                     ENEKQNGSGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYNQLKNENIVESIKSETI"
     gene            complement(9776..10459)
                     /locus_tag="L3M60_002132"
     CDS             complement(9776..10459)
                     /locus_tag="L3M60_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226793.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLNEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     TILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            complement(10808..11740)
                     /gene="blaCDD"
                     /locus_tag="L3M60_002133"
     CDS             complement(10808..11740)
                     /gene="blaCDD"
                     /locus_tag="L3M60_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBN6226794.1"
                     /translation="MKRKKNFIWTTILLVGIVMVMYYAGKKHNDINQKTDKNYIKSDL
                     NKSEINNDNNKKERVNIVNYSDCFEGISGGAIFYNSRNQEYNIYNKDLIETRASPCST
                     FKIISTLIGLEKGVITSKKSVIGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLINK
                     VDAKSVQSILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMVKIF
                     EGDTNFKKEHINILKDIMKIDVSDKNINVYGKTGTGFNGKNKRVDAWFVGMLESKDDT
                     YYFAIKSDDSNKEIAGPKVKEIAINIIKKYYSVM"
     gene            complement(12280..14394)
                     /locus_tag="L3M60_002134"
     CDS             complement(12280..14394)
                     /locus_tag="L3M60_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HBN6226795.1"
                     /translation="MFEDKQVINCDTIASNSKHNMYEHDTELLTFFPDEIAHLIDINN
                     KLDDAFKKAENLVDKLDKDYMDTKMYMVKNRGEIDPHEMFQNEQGLKQIDNYGAFMVK
                     VRDKIDKIKDSPYFARIDFRLKDMDDESKYYIGRFAFDYEDELIILDWRSPIASMFYD
                     YEIGKAGYDAPIGWVDGEITRKRQFKIKNGKLEYALESSINIQDDILQKELSHTSDEK
                     MKSIISTIQKEQNQIIRNDKAHTLIIQGVAGSGKTSIALHRIAFLLYRFKDKISANNV
                     IILSPNKVFGDYISNVLPELGEEPLCELSFENIAEVQLDRVINFESEKDPLEINDAKW
                     AERVRFKSTFDFVKLIDYYIKQMPNKIFIPKDYNFGSFTAKSDWIQSRFEAYNRYPVK
                     KRLEKVAEDIHYKFESDNIMEEDLPKLKSILKSLNGMLTIKNTLTLYKDFFKQMNLSN
                     MFVMATKKTLEWSDVYPFIYIHAAYEGIQEDKIIRHVVIDEMQDYTPIQYAVINLLFK
                     CKKTILGGFGQLVNPNHTHTLDDMRQLYNEGELVTLNKSYRSTFEIINFAKKVQDVSS
                     LEPIERHGEEPALVKCTNKQDEVSKIKIEIEEFKKSDNATLGIILKTDSDAEVIYNAL
                     KQEYSVNLISSESSSFTKGVSITSIKMSKGLEFDEVIIPSVNSKTYYSDYDRSLLYIA
                     CTRAMHKLKLTYTGELTQLIDM"
     gene            complement(14641..15501)
                     /locus_tag="L3M60_002135"
     CDS             complement(14641..15501)
                     /locus_tag="L3M60_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6226796.1"
                     /translation="MSYKVINVITAIDYIEEHLSEKLDLDIVANAVHYSKYHLHRTFT
                     TSVGLTMHDYIKRRKLTEAAKLLVFSKKPIIEIALIAGYESQQAFTSIFKAMYKKSPN
                     KYRKEQEFYPLQLRFVLKKDNLFSKNVLELEKEIKLANMSDIPLWMNLVRLVIDGFPN
                     LQEEEYIYQLEQYILEERALILKLNNVAIANMVFNRETRSIDFFGIHPQYRNSEIAQV
                     FLKKVIEDFLIDTDISITTFREGDKADTGHRDMIKKLGFAEAELLVEFGYPTQKFILP
                     SVEETDTRPN"
     gene            complement(15692..16306)
                     /locus_tag="L3M60_002136"
     CDS             complement(15692..16306)
                     /locus_tag="L3M60_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBN6226797.1"
                     /translation="MNEKWLEWAIELQSIAQAGITYGKDIYDRERYERIREISAEIIA
                     YKTDISTEKVRNLFCNESGYQTPKLDTRAAIFENGKILLVKENTGKWSLPGGWVEVNL
                     SVKENTIKEVKEEAGLDITADRIIAIQDRAKHNLPIYAYGVCKIFVLCTVIGGEFKEN
                     IETTEFSYFSENELPELATEKNTEEQIKMCFKACRTDTWTVIFD"
     gene            complement(16374..16736)
                     /locus_tag="L3M60_002137"
     CDS             complement(16374..16736)
                     /locus_tag="L3M60_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN6226798.1"
                     /translation="MKIYDILTRFYIQDIEKAIPFYENLLKEKCSLRFSYKEVGLELA
                     QIGNVLLLSGSDEALKPFVETKSTFMVDSVDEWRSYLLDNGAVVVRDKKKVPTGYNMT
                     LRHPDGTIIEYVQHTKQE"
     gene            complement(16956..>17232)
                     /locus_tag="L3M60_002138"
     CDS             complement(16956..>17232)
                     /locus_tag="L3M60_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HBN6226799.1"
                     /translation="DLAELDVEVGFPVATYIDGKNEILQRIIPSQKIITAIDLGPYEL
                     QDPTLEDLFSWANSNGYKLHGDIYYQYLNDINRPASEYLTKMMLPII"
BASE COUNT         5854 a         2794 c         2032 g         6552 t
ORIGIN      
        1 aatttataag atatagaacc atatttatat ttttatctct tgatttgttt taattacata
       61 tatttatttt ttatcttatt taataattac tattacttga atatttactt ctttgagtaa
      121 atttcatagc taacaaagct aaaactatta ttatagaggt caaaatacaa atataattca
      181 tattaaaaaa gcttccatct ctttcttcta tataagtcac aaaactaata aaccacctta
      241 cagggcttat aatactaatt ttttgcaaca tttctggcat tttttcaact gagatgaatg
      301 aacctccaat aagtgataat acaagtgata tacatgtaga aaataaattt gcatgcatct
      361 ctctattgaa taaagaagat acaaatgttc caaaagaagt tgccaaagct actacccata
      421 ttcccattaa aattaaatct atataatcca taccaatatc aatattaaat aacttaatta
      481 tagatattga aactatgtac tgaaaatacg ttattgtaaa gttgtataac atattcccta
      541 gcacatatcc tcttacattt tgtggagaat acagcactct tgtaaatgta cctgagtttt
      601 tatctttaat aatcattgat gctgttacag tagatgttat cattaaaaat aatacgataa
      661 atgcaattat tctttgtaaa actcctaaag tattttcttg taatttactt atatctacag
      721 gaactgggtc ttttttatat gtttctatta aatactttaa tgtatcattc gtatttttat
      781 tttttataga atataactta aaaccattct tattaaaata tatgacagct gaatacttgc
      841 ctgttataat atcagtttta attgttttaa aatctgcaat ctttgtatta atcccttgag
      901 tattttctaa agccattatt gtattttcat aggttctttt ttcagattcc tttagaactc
      961 gttcgttttt gtactcatct aaaataccaa tattaaaaaa tggtttactt atgtaatttg
     1021 ccaatactcc caaaattact ataattatag gaaataaaag tgtaactaaa aatactattc
     1081 tcttatttat atttcttttt aagttaatat taaaaacatt tattacattc actatataaa
     1141 gacccccttt ttaaaaaact tgattgtaag tattgtcata aatatgctta caactacaaa
     1201 tatgcatgat atattaaaaa ggagttgtaa attattatca tagatacaag caactatgcc
     1261 tctattaatc catgttaatg gtgatatatt aatgattaat gaaataactg gattcaatga
     1321 actaactgga aagaacactc ctcctaagaa tccaaaaata actatcggta tattaataat
     1381 attacgtaac atatctaaat ttttacaaac aagacccaaa aacaacccca tagatactac
     1441 cacaatagat tcacacatta aaaagaaaag aacagctaaa aattttccac caaaactcat
     1501 acgaaaaata cattttaaca ccactaaagt taccaaattg catatcgaaa gtactgctgt
     1561 acaagaaaaa agttttgaaa taactaaatc tactattgaa ataggagaaa tcatataacg
     1621 atatgatgtg ttagaaattt tttcatcttg tcccgcatat gaaacactaa taattcccat
     1681 aagaacacaa aatggtattg taacaatagt ataataatta tatgatgtaa attcctcacc
     1741 ataggacttt gatgtcaaat atcctaataa cattatgatt attactggaa aaacaatatt
     1801 gtaaaacaga ataaaaaaat ctttacttct tcgttttatt cctaatttaa acaatggtaa
     1861 aaaacatctc atctatttat tcctccaagt ctctcaaatc tgtacctgta agatataaaa
     1921 aaatatcttc taatgtctta caaccaaatt ctaaatattt atttttatat aaactcttgt
     1981 ctaaatcttc tataattatt cctttatcta ttaaagcaat tctattacat aaggtttcaa
     2041 tttcttccat ataatggcta gtatatataa ctgtcgtacc attagtatta atgcatttga
     2101 ttgcctccaa aattttattt cttgattgag ggtcgatacc tactgttggc tcatccatga
     2161 tgagaatctt tggactatgt gcaattgcac aagctatgtt tagtctacgt ttcattccac
     2221 cagaaaattt tgatggaaac tccttatggc tttcccaaag accaacaaat tccaaagcat
     2281 ctttagttct ctctttaagt tcattccctt taaatccata taatgagcaa aaaaatttaa
     2341 cattatcata tgcacttaaa tcttcatata ttgctaattc ttgtggtaca actccaagtg
     2401 aacttttaaa aatattttgt tgattcttaa tatcttttcc tttatagtat atgcttccac
     2461 tatctggaga cacaatcgta gtaagcatat tgatagatgt acttttccct gctccatttg
     2521 ctccaatgaa acctaaaata tcgccttcca ataacttaaa actttctatt gacacaactt
     2581 ccttattgtt atacttctta gataaatttt taatctctaa caatatatta ctcatttata
     2641 accccccaat atttaaccaa cattatatat ccaatattgt atatataatt tttttaaaac
     2701 acgagtattt tacccgtgtt attgtaataa aatttattac ttattttgta attttagtat
     2761 tatcttatct atgttttcta tagctttatc tgggttatta attatttcat ctctaagtct
     2821 ctgaacttct aataactcat ttgtcgtatc agatgtaaat aaaaaatctt cttctatatt
     2881 tgaaaaatct atagatttaa ttatattctg tgtctcgcaa ccaacggata tatacttatc
     2941 aatatcattt aatatttctt catgccataa aatttgataa ttcagattat ctatagtcat
     3001 atcaaatgca attttttctg ttgcaagact ctttttatca atttttttaa tctctttcca
     3061 attttcccaa tacttcttct gctctataag atattttata tgtttttcaa gatttttttg
     3121 caaagtatcc ttaggtaata catccaaaat attatgtaat aggaatttat ttgaaccggt
     3181 tggaactatt ggcatctgaa gctctttttg tatttcttcc cttaattcta atttaccact
     3241 ttgagtaatc tcatagattt tacgaatcct agctcctgtt ttttcttctt ttaaaactct
     3301 aacgcctcca tctttttcta attttgccaa tgcataatat attgacccag attgtatttt
     3361 agtccatcta tctgcaccag atacttgtaa aaacttttga atctcatacc catgtgttgg
     3421 cttaatattt agataatata aaattaaagt gcgtatcata aaaatccacc acctatactc
     3481 tatcttggat attatattat caccaatctt ggatatagtc aatagataac ataggatata
     3541 ttacaactta ttatttttat tatttaatac aaggagttcc atgagacata catatccatt
     3601 catattctaa attatccaat tctcttatag atttcatcat ctttttagaa tcccaattcc
     3661 agggattagg gtaagaaact atatttccat tcttgttttt gactaaatca cctgcaaata
     3721 atacatcttc aaaaatcatg cagacatgcc ctggagtatg accaggagta tggataattt
     3781 ttatattccc aatctgcata ttctctccat aaggttcaac atccttaatg agccttcttg
     3841 aaattgtctt tccaataaac ttcttaaacc caggtctatc tttctctcct ataataaaag
     3901 gtatatcttc tgtatgagcc catacctttg ctcctgtttt ctcttttaat aatttaatat
     3961 tagacatatg gtcaacatca tgatgagtta ataaaatatg cttaatatct gtaagtttga
     4021 taccaagtgt ttttaattct ttaattatat ttttccccat aaaaggaaga cctgtgtcta
     4081 taagagtaat ctctttatca aacacaataa aacaatttga gccacgagta ctatctaacc
     4141 ttaatacatt ttcttttatt ttcataaata aattccccct atttggtatt ttgcctatat
     4201 aattaattaa tgaagttata aatagctata atacttgata ttaattttat tttcattaaa
     4261 gtatactaat agtatacaaa tatattatat ttacacaagt ataccaccag tatactttat
     4321 tatcaataga aaggaataat ttatatgtca gaattatcaa aaagagataa agaaaaaata
     4381 caaagagaaa atgaaattat tgataaagca gaaaaattat tttctcttaa tggatttgat
     4441 aatacaacta tgaatgagtt ggcaaaggaa gtcgaatata ctaaacgcac catatacaag
     4501 tacttttcat gtaaagaaga tttattcttt gctgttgtct taaggggata taaaagactt
     4561 tgggataatg ttaaaataga aagtgcaaaa ggtaaaacag ggtttgaaaa aataaaactt
     4621 tcatactttg cttttcataa attttactct gtagaacctt ctctattatg ccttatggga
     4681 atgattggta ttgtaaactc taaaaaatcg gatactgaga tgctgtttaa agaaaagttt
     4741 ttttctttta ataaatttat gtttgatgaa atacaagaaa tgtttgaaat tggaaaaaat
     4801 gatggaagta ttaggtatga tattgaaata tctactctta tgtattcatc aatatttaca
     4861 cttacaggat tttttaattt attgtctgta acaagaaaat catacctgaa caattttaat
     4921 atagatgagg aaaaatttat tgaaacaacc cttgccattt taatagactc gattaaagca
     4981 tagacctaag tatataaatt aaaatttata tacttaaatg gtaatcttta ctatatataa
     5041 tataaaaaat attaagcata aaactaataa tttaaattaa tgaaaggact aaaagtaaat
     5101 ggctaaaata cttctcttag aagatgatga tacaattacg tttgggataa agacttccct
     5161 ggaaaataaa aatcacattg tatgttgtta taatacgatt gctgaagcta aaaaaaattt
     5221 tgataaaact actgatttaa ttctgcttga cttaaatcta cctgatggta ttggatacga
     5281 attttgccaa tacgttaaaa gtatagaaga tactccagtg atatttttaa cagtccgtga
     5341 tgatgaaaaa gacattatac gtggattaga tatgggtgca gatgactatg tggttaaacc
     5401 atttttgctt tcagtacttc aatctagaat taatgcagtt cttagaagag ctcattctaa
     5461 tactaataat actattactt gtggaaatat aagtttgaat aaagttgaag tcaaggcata
     5521 tttagatagt gttgaagtac ctctgacagc aggagaatat aagcttcttc taagtcttat
     5581 ggaaaataaa aatagagcta tgactcgtac aaaattacta gaaatgctct gggatgtaga
     5641 tggtaatttc gtaaatgaca acactcttac tgttacaata aagcgtctta gagaaaaatt
     5701 aaaaaatcca ccttgtataa aaacattaag aggtatagga tatagaatgg aggacagcta
     5761 gtgaaaactc caagaaatat caacagtaca gttgttttta taatattctg cttaattgta
     5821 acttttttat tgagtgtaat tctaacaact atcacttctt ttaatgaata caatgtgctg
     5881 tctaaggttc ttgatgcatc atatgagtac aatcctaaat ctacagaaag tatactaaaa
     5941 tcactgaaaa attctagtga tgaggatttt aaaacaggaa atgaaatttt atcaaaacat
     6001 ggatattcta aaggtacatt ttggagtaaa aacttttttt attttctagt ggtatcgttt
     6061 ggtataattt caacaatggc tatagttgta tatataatca agtatagaac aaataagcaa
     6121 aaattgagta gaataaacat gcttactcga tacttagaag aagtaaattt aggaaaagag
     6181 tctgtacttt taagatatga agatgaattt tcttatcttg aagatgagat atataaaaca
     6241 gttggggaac ttcgttgttc aaaagaattt gctctaaaag aaagacaaac tctttcagat
     6301 aaccttgcag atattgctca tcagcttaag actccaatta cttctatgtc acttatggca
     6361 caattacttt cagaaaattg tccagatgaa gaaatagatt atattaacag gcttacaaag
     6421 caaatatcaa gacttgagag actagtttca tctttactta ccttatcaaa attggatgcg
     6481 agtactttaa tatttgaaag caaacttata gatgtacatt ctcttcttac ttatgcaatt
     6541 gagcctatag aaggttcttt aagagaaaaa catcagacat ttactatcaa aggttctcct
     6601 tcaatagaat ttgaaattga tattaattgg actttagaag ctcttttaaa tattcttaaa
     6661 aattgtagtg agcatataga agctggtgga catatcattg cttattatag tcaaaaccct
     6721 ctatatgttg agattatcat agaagataat ggtaaggggt ttgctcagga agagctccca
     6781 tatattttta atagatttta tcgtggtaaa aacgctagta aggacagtat tggtataggg
     6841 cttgctctat caaagtctat tatagaacgt caaaatggta ctctacatgc agaaaattgc
     6901 cataatggtg gtgcaagatt cataattaag ttttacacat aaaatactgg attataggta
     6961 aaatacccat aatccagtca tatctatatc ctatgttatt tattatttta gtaatccgct
     7021 atttatatct aagattttat taaataaaca atttaactat ctctatactc gtaagtattc
     7081 ttgaattgaa accataaata agttcagata acttttattt atattgtttc actttttata
     7141 gattctacta tattttcatt cttcaactga ttgtatcctg tcatatatga tgctattaca
     7201 ataaaaagta tcaatatagt aaaacctatt attgtagata taggtgcaaa tggtaaatac
     7261 tctataaaat atatttcatt tatacgtaga aaagtatata ctattcctat ttgcacagga
     7321 atactaagta gtaatggtgt aattcctaag aataaacctt ccaaaacaag catctttttt
     7381 atgccatctg gagataatcc aacagaacgc aacatagcaa actcttgtcg tctacttcta
     7441 atatttcctg acactgtagc aagtacattt gataatccaa tcatagccaa taaacctgta
     7501 ataaaaccta ctatcaaatt catagtcttt cttccactac cattttgttt ttcattttct
     7561 acaacatcct caatactata gtctcctgaa ccataatctt tattacaaat agactctaat
     7621 tttcctctta ctctaggaat atcctcacga ttatctactt taaacaatcc tatcagtcta
     7681 tttgaacgtg acctacctac ttcagcataa tttttagata tttcttgcac catagatatt
     7741 ggtactattt gcattaatgt atatcttgaa ctagtatcta ttattttagg aagtgtgctt
     7801 gtaatagctc ccactttcat gttgtaagta taatcccctt tatcttcatc atatattttt
     7861 tcatttaatt taatgttatc tcctattgaa agatttaaat aatctatata tactttattt
     7921 ctacgtgtgc tacgattaac atcttcctcc ttattatata caattgaagt tggatttttt
     7981 gtatcataaa ataattttgg gtctacagag atttgtctgc aatattctgc gaaactctca
     8041 tcatcaagcc caattatatt tgaaattata cgaaacttgc catcacgctc tataggagag
     8101 tactttttag atgaaactat acttttaaat cctccatttt tcgaaagttc ttttgaagca
     8161 tctttttcag tcagccaagt ggtagcagac atcttttgat aatatagtga gttctttaca
     8221 ccttctgtat tatctaattt ttcaatgaaa tcagactcta caggttcact attttccaaa
     8281 taaacttcta tatctctttg gtctttataa ttatcacttc cataaacagc ttctgctcct
     8341 tcttgatttg caattatatg caaaaaacct gtcagtaata aaaaagataa tgttaaagat
     8401 attgttgcag tacgataaga ttttttacga gcacgtagtg catttgatgc aagttctcct
     8461 tctattccaa atatctttcc taatgaatat ttatgtgact ttttaatttt aacactatct
     8521 ccttgtcgta ttgcttctat aggagatatt tttgatactt ttcgtgctgg tatgagtgta
     8581 gaaatccaga ctgttactat tgtaagtaaa actgctggca gaaaagcagg aataccataa
     8641 gtgaatacta cttctggagc atcagtatga taattaactg aattaatata gactataagc
     8701 ttattacaca ataaccaccc taaaaataaa cctattggta atggtataat agtcaataaa
     8761 aaaccttcaa acactacaga ccttttaatc tgttttggtg atgctccaac actagataat
     8821 attcctaatt gtgtaagtct agcatttgca gatagtgaaa atgcgttgta aattaccatt
     8881 acaaacacgg ctactgtaaa aattgctatt gtaagataca taataggagt tgaaaatttt
     8941 gatatattgc tcatagatgc tttctgttct ggagaaaaga ctaacatttt taaaagataa
     9001 ttactattgt atttaagatt gtattttcca tattcattag tcttccatcc caatgattta
     9061 gcaagttttg gaagttcctt atatgtgtct cttatatttt taaatcgcaa gtacacagtt
     9121 atactgtcat taggttttat acttgtttta tcaagatatc caagagctgt atatgcaggt
     9181 acaactgatg aagtagcaac atctaccttt cctactattg tcaatgtagt ctttgaaatt
     9241 tttttaaaac tttccccttt cgattttggg gctacaggtt caagaggctt tccatttagg
     9301 attctatctc ctattgggag agtaatttta tcaccaattt ttacacttgg attattctca
     9361 aaatattgtt ttgatagtgc aatttcatta gtattacttg gtcgacgccc ctctattata
     9421 gaatcttttt ctggcatatg attccaatac tctgatgtag catttctata aactatataa
     9481 tctcttttgt cattattaat tttgcctaca taccattgtc cttttaccat tacatcatca
     9541 actgaagaaa aattttcaat taacgataag tcttttccat aagtatcgtc aaacaattca
     9601 ccatgccagt taccctcttt ctcaattgaa agtcttataa aatctgtcca catggtatac
     9661 aacaatccac acaaggaaga catcatggtt gtggtaagta atatggctaa cattatagct
     9721 atactactac gtttattgcg tcgtacaaag tctaaggtat attcttgaag aatattcatt
     9781 tcttcaccac cttttcagag acaatcactc catcttccat agttacaata cgatttgcct
     9841 ctagtgctat tttctcatca tgagtaataa gaagtattgt ctgattaaat cgtttatttg
     9901 ataagtttaa caaatctaca atttcttctg aattttttct atcgagatta ccagttggct
     9961 catctgctaa taaaatagtt ggtctataga ttaaacttct agctatagat accctttgtt
    10021 gttgcccacc tgaaagttga cttggaagat gattcaatct atcagataat cctaatatag
    10081 atacaatctc agagaatcta tcttcatcca cctttctttt atcaagtaac atgggtaata
    10141 atatattttt tctaacatca agagtaggta taagattata aaattgataa ataagaccta
    10201 cttttctacg tctaaaaact gcaagagctt cttcatttaa gcttgatatt tctgtatctt
    10261 caacataaat ctttccagat gtaggtctat ctactccccc aagcatatgt agcaatgtag
    10321 atttgccaga accagatgct cctacaatgg atacaaaatc acctttttga actgataaat
    10381 ttacattatt aagagctgtc actctggttt gatttgaacc atatattttt gtgagatttt
    10441 cacattttaa tatttccata tacactcatc tcctttatgg ttaaatttta gcaacctaaa
    10501 atgacattat agtgacatga atacaaatat cgattactat taaaaatatt gtttagtttt
    10561 tgaacattat ttaaaattat aatacaaaac aaaaactatg caatatattt tatttatact
    10621 tttaatatat tgcatagcct ttaaaagata ttaactttaa tatctataag tattcaattt
    10681 aataatcaat agcaagtatt ttatatacta gaggagtttt gctctatgta aactcaattt
    10741 agtaaaatta tttaataaat atttacagac tataggtaac caatatttac aaataaaatt
    10801 aagtttatta cattacacta taatatttct taattatatt aattgcaatt tcctttacct
    10861 ttggtcctgc aatttcttta ttggaatcat ctgatttaat agcaaaataa taagtgtcat
    10921 ctttactctc tagcatacca acaaaccatg catctacacg tttatttttt ccattaaatc
    10981 ctgttccagt ttttccatat acatttatat ttttatcact tacatctatt ttcataatat
    11041 cttttaaaat attaatatgt tcttttttaa agtttgtatc tccctcaaat attttcacca
    11101 ttgtttgcac ttgttctttt ggagatattt gtaatgaaga ttctaaccaa aatccattta
    11161 aatgcccctt tccatttttt aaatctcctt cccattcact aatatcacag ttcccatact
    11221 ttaaatcatc taaaatactt tggacagatt tagcatcaac cttatttatc aattttttgt
    11281 agtaccaaac acaagactcc ttaaatgcct cttctaaact aagattttta ttccaatttt
    11341 tatttggata atttgtacca tcatacccaa taactgattt ttttgacgtt attacccctt
    11401 tttctaaacc aattaatgtt gaaattattt taaaagtaga acaaggtgaa gctcttgttt
    11461 caattaaatc tttgttatat atattatatt cttgatttct gctattataa aaaatagctc
    11521 ctccactaat accttcaaaa caatcactat agttaactat attaactctt tcttttttat
    11581 tgttatcatt attaatttca gatttattta aatcagattt tatgtaattc ttatcagttt
    11641 tttgatttat atcgttatgt tttttacctg cataatacat taccataact ataccaacca
    11701 acaagatagt tgtccaaata aaattctttt ttcttttcat aatttatatc atctcccata
    11761 tttaaattta taacctactt ttaaaatagt agacattatt tgtaatatac ttcaatctag
    11821 gtgctactta taaaaaataa gactaaagga ataactatat atcttatatt ttcttcacct
    11881 agcacaacta tgtcgattaa acctattacg aaaataatta ggtaactatt aatagcattt
    11941 tgtcctcctt ttaatttgtg atatagtatt tgattctact tttaaaactt actattgtaa
    12001 tatttaaata ttacaatagt aagttttaaa agtcaagtta gtttgagaaa gttattttaa
    12061 ttgtataatt gttattacat ttataaaata tggcacattt aaaattattt tggtattggt
    12121 agtattaata taataaatat tataaaagct ataaattata aaatttaata taatttatag
    12181 cttttactct ttcaaatatt attttttatt gacttattaa tcaattttat taacaggtta
    12241 catttatata agtaaacact taaataataa atatataatc tacatatcta taagctgagt
    12301 caattcacca gtatatgtca acttaagttt gtgcatagct cgtgtacaag caatatacaa
    12361 caaactacgg tcatagtcac tatagtatgt tttactgttt actgaaggta taataacttc
    12421 atcaaactct aaaccttttg acattttaat agaagttatg gacactcctt ttgtaaaact
    12481 agaactttca gaagatatta agttaacact atactcttgt tttaaagcat tatatataac
    12541 ctctgcatca ctatctgtct ttaatataat acccaaagtt gcattatcac ttttcttaaa
    12601 ctcttcaatt tctattttta tcttagatac ttcatcttgt ttattagtac atttaactaa
    12661 agctggttct tcgccatgtc gttcaatagg ctctaaagaa gatacatctt gaaccttctt
    12721 tgcaaaatta ataatctcaa aagtagaacg ataactcttg ttcaatgtaa ctagttctcc
    12781 ttcattatat agttgtctca tatcatctag tgtatgagta tgattaggat taaccaattg
    12841 tccaaagcct ccaagaatag tctttttaca cttaaaaagt aagtttatta cagcatattg
    12901 aataggagta tagtcctgca tttcatcaat aacaacatgt cttataatct tatcttcttg
    12961 tatcccttca tatgcagcat gaatatatat aaatgggtat acatctgacc attcaagagt
    13021 tttctttgtt gccataacaa acatatttga tagattcatt tgtttaaaga aatccttata
    13081 tagtgtaaga gtatttttta ttgtcaacat tccattcaaa cttttaagta tactttttaa
    13141 ctttggtaaa tcctcttcca taatattatc tgactcaaac ttataatgaa tgtcttcagc
    13201 taccttctct aatcttttct taactggata tctattatat gcctcaaatc tactctgtat
    13261 ccagtcactt ttagctgtaa aacttccaaa attgtagtct tttgggataa aaatcttatt
    13321 tggcatttgt tttatataat aatcaataag ttttacaaaa tcaaatgtag atttaaagcg
    13381 cactctttca gcccatttag catcatttat ttctaatggg tctttctcac tttcaaaatt
    13441 aataactcta tctaattgta cctctgctat attttcaaaa cttaattcac ataatggctc
    13501 ttctccaagc tcgggaagga cattggaaat atagtctcca aagactttat tcggtgatag
    13561 gataattaca ttgtttgctg atattttatc tttaaatcga taaagaagaa aagcaattct
    13621 gtgtaatgct atagatgttt ttccagaacc agctacccct tgaataatta gtgtatgagc
    13681 tttatcattt ctaataattt gattttgttc cttttgtatt gttgaaataa ttgacttcat
    13741 cttttcatct gaagtgtgac taagctcctt ttgaagaata tcatcttgta tattaatgga
    13801 actttctaat gcgtattcca attttccatt tttaatttta aattgtcgtt ttcttgttat
    13861 ttctccatcc acccatccta ttggagcatc atatcccgct tttcctattt cataatcata
    13921 aaacatacta gcaattggag aacgccagtc aagtattata agttcatctt cataatcaaa
    13981 tgcaaatcta ccaatataat acttagattc atcatccata tcttttagcc taaaatctat
    14041 tctagcaaag tatggagaat ctttaatttt atcaatctta tctcgaactt ttaccataaa
    14101 agccccatag ttatctattt gttttagccc ctgttcattt tgaaacattt catgtgggtc
    14161 tatttcacca cgatttttta ccatatacat tttagtatcc atataatcct tgtcaagctt
    14221 gtcaacaagg ttttcagctt tcttaaaagc atcatctaac ttattattta tatctattag
    14281 atgtgcaatc tcatctggaa aaaaagttaa taattctgta tcatgttcat acatattgtg
    14341 tttagaattg gatgcaatag tatcacaatt tatcacttgt ttatcttcaa acatttaaat
    14401 cctccttgta attaagtact tacactgaat cattatactt aattgtgctt tgaagttttt
    14461 aattattctt gctatttaac ttaaatatta actttaaaat tcatttttct aaaactaaat
    14521 ctttttatac ttataagcat aagtatattt acaaacaaaa tatgttgaaa ataaaaacac
    14581 tcttatatat aaattaattc aaacaaaaaa tatgtagtaa actttttatt taatctacta
    14641 ttaatttggt ctagtatctg tttcttctac agaaggaaga ataaattttt gagtaggata
    14701 cccaaattcc acaagtaatt ctgcttctgc aaaacccaac ttctttatca tatctctatg
    14761 acctgtatca gctttatcac cttctcgaaa agtagtaatt gagatatcag tatctatcaa
    14821 aaaatcttca ataactttct tcaaaaacac ttgtgcaatc tctgaattac gatactgtgg
    14881 atgtattcca aaaaaatcaa tgcttctagt ttctctatta aacaccatat ttgcaatagc
    14941 tacattatta agttttaata ttaaagctct ttcttctaaa atatactgtt ctaattgata
    15001 tatatattct tcctcttgca aatttggaaa tccatctatg actaatctca ctaaattcat
    15061 ccataatggt atatcagaca tatttgcaag tttaatttct ttttccaatt ctagtacatt
    15121 ctttgaaaat aagttatctt ttttcaaaac aaatcttagc tgcaaaggat aaaattcttg
    15181 ttctttccta tatttatttg gagatttttt atacatagct ttaaatatag aagtgaatgc
    15241 ctgttgactt tcatatccag ctatcaatgc aatttcaatt attggtttct ttgaaaatac
    15301 caataatttt gctgcttctg tcaactttct tcgctttatg taatcatgca ttgtcagacc
    15361 aacagaagtt gtaaaagttc tatgtaagtg atattttgag taatgaacag cattagcgac
    15421 tatatctaaa tccagctttt ctgataaatg ctcctctata tagtcaatgg cagttatgac
    15481 attaattact ttataagaca ttgcagcttt cctcctttta tccatgcaac attatatcaa
    15541 ttataactta gttatattta aattatcttt ctattgattt gttttatcgt gtcttgtaga
    15601 gagtgtaaaa tggcgtattc agtgtcaatc aatgtgccgt ctacataaaa gacgatttct
    15661 ttgtaatcca ttaatttctc cttaaattag cctaatcgaa aatcactgtc caagtgtctg
    15721 tacgacatgc tttaaagcac attttaatct gttcttctgt atttttttct gtggcgagtt
    15781 caggaagttc attttcggag aaataagaaa actccgtagt ttctatattt tctttaaatt
    15841 cacccccgat aacggtacaa agaacaaata ttttgcatac tccgtaagcg tatataggta
    15901 aattgtgttt cgcgcggtct tgaattgcaa taatcctgtc ggcggttata tctaatccag
    15961 cttcttcttt gacctcttta atagtatttt ccttgactga taaatttact tcaacccaac
    16021 cgcctggaag cgaccacttc cctgtatttt ctttaacaag aagtattttt ccattttcaa
    16081 aaatagcagc tctagtatca agttttggag tttggtatcc actttcattg caaaaaagat
    16141 ttcttacttt ttccgtggat atgtcagttt tatatgctat aatttcggcg gaaatttctc
    16201 gtattctctc ataccgctct ctgtcgtata tatctttccc ataagttatt ccagcttgag
    16261 cgatgctttg caattctatc gcccattcca accatttttc gttcataaaa tcacctcccg
    16321 ttttccgaag aatatattat aattagttac tgattagtaa aatctatttt taactactcc
    16381 tgttttgtat gttgaacata ttctataatt gtaccatctg gatgtctcaa tgtcatatta
    16441 tatcctgtag gaactttttt cttatctctt actacaactg ctccattatc caataggtaa
    16501 cttctccatt catcaacaga atcaaccata aaagtagatt ttgtttctac aaatggttta
    16561 agagcctcat cagaaccaga taataataaa acatttccta tttgtgctag ttccaaccca
    16621 acttctttat aagaaaaacg caaagaacat ttttctttta aaagattctc ataaaaaggt
    16681 atggcctttt ctatatcttg aatataaaaa cgtgttaata tatcgtatat tttcatattt
    16741 tccccctata tctctatatt atttataaag tatatcactc tacaaattat atagcaataa
    16801 attaagcatt accttttgag cattttaagt gcactttcac aatatgtttg gggtacatca
    16861 gaatacataa attaaatctc tttgatatta tttagtataa aatttataca gtaaatgtcc
    16921 ttattatcaa ctaataaacg agaatctttt actttctata taatgggtaa catcattttc
    16981 gtaaggtatt cactagctgg acgattgata tcatttaaat attggtaata tatatcgcca
    17041 tgaagtttat atccattact atttgcccat gaaaataagt cctctaaagt agggtcttgt
    17101 aattcatagg gccctaaatc aattgcagtt attattttct gtgaaggaat aattctttgt
    17161 aaaatttcat ttttcccatc tatataagtg gccacaggaa accctacttc aacatccaat
    17221 tctgctaaat cc
//
LOCUS       DAFNNB010000077        17192 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0078, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000077 DAFNNB010000000
VERSION     DAFNNB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 17192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17192
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0078"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            150..611
                     /locus_tag="L3M60_002139"
     CDS             150..611
                     /locus_tag="L3M60_002139"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226800.1"
                     /translation="MKIKKEYIEKVENILKMLGDKYRKLRILKKEIAILKHKENYREI
                     NFEELGFKVKRSTKGIDDIVVTIEDTIYNKETEIEIIERKLEFYNIYLKELSELEQKM
                     IELIYFYNWDEKLPITKIAYELNYDRTSLYNKKAIAINKIALMIYGDEALA"
     gene            788..1336
                     /locus_tag="L3M60_002140"
     CDS             788..1336
                     /locus_tag="L3M60_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN6226801.1"
                     /translation="MNFVEPIRDLEKLESMCAYLEEANMRNNVLFSMGIYTGLRVGDI
                     LKLRIKDVHDKRFITIKESKTGKTKPIEVNPILKRILKEYTKDKNQNEFLIKSREQTN
                     KAISRVMAYKIIREVGEMFGIDNLGTHTMRKTFGYHYYKRTHDVVTLQKLFNHSSATI
                     TLRYIGIEQDDLNKAYRNIKYF"
     gene            1509..1781
                     /locus_tag="L3M60_002141"
     CDS             1509..1781
                     /locus_tag="L3M60_002141"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226802.1"
                     /translation="MNKYDRFKCNNCGRKIAVSNKELRTKVIDNTTVTYFMCRRCFEK
                     FIVSCEDDYIKHKKEEYKKLKDDEVKLLQDMKEYSDGLGELYINEL"
     gene            1771..2103
                     /locus_tag="L3M60_002142"
     CDS             1771..2103
                     /locus_tag="L3M60_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBN6226803.1"
                     /translation="MSYKHITLDRLKELILIDRLDIFYRSTDWRKLRKKAIERDNYEC
                     QVCKKKKKVSKAECVHHIREIKEYPEGALKLNNLTSLCNLCHNEIHEKILKINQEKKK
                     KFFSDERW"
     gene            2276..2782
                     /locus_tag="L3M60_002143"
     CDS             2276..2782
                     /locus_tag="L3M60_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HBN6226804.1"
                     /translation="MDDNEELEIRRDVQNDYVLGMTYKEMSAKYNIPENTLKSWRKRG
                     GWTRNCTPKKGCVKNGLQESGNNLKNSIRKDLLRQIKENNMTEEYFKDLVEDYLKMWD
                     IKNELIEDVKTRGVQVKYKNGQNQWGYKKNDSVSELIKYNAQMLSLLDKLGIKATPKV
                     LGDDDIEL"
     gene            2766..4394
                     /locus_tag="L3M60_002144"
     CDS             2766..4394
                     /locus_tag="L3M60_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBN6226805.1"
                     /translation="MILNYKYNKYIDEYFNLVDKGEIVLCKEMELAVKKIKKDLKKPN
                     IYIDHEKVEKAIEYMEKYFYKMYAWEKFVVALVVGCFEGDELLYDEIFIMMGRGNGKN
                     GFISQLANYLQTHLHGVKNYGIDIVAMSEEQAKTSFDDVYNMLSEHPAICKKFFNRTK
                     EVITFKKTNSYLKFRTNNAKSKDGLRPGCIIFDEVHAYENYDNIKVFTSALGKVKNPR
                     VFYITTDGNVRGGVLDDFKEESKQILNEEIKNSKMLPLIFKLDSIEEMENIDLLEKAN
                     PSIKYNSDLRTQILKDIDKAKNRPQMMIELLTKRFNLPAQNLATVVAEWDDIVATNEE
                     LPDLKGFSCIGGLDFSSIRDFTSVVLLFKKDKKRYIMHHTFICYKSLEITNFKFDIEL
                     AKQKGLCTIIYDETIKAEYIADWFIEKAEDYNILKICCDSYRAAHVKEEFANKGLPIE
                     EVRNGSITHAKIAPLVEQIFAERTIKWGDDMMMRWYTNNVYVDTDKKGNKTFLKIEPI
                     KRKTDGFFAFLHALSKDGELMQESTIKFYDCYTY"
     gene            4458..5603
                     /locus_tag="L3M60_002145"
     CDS             4458..5603
                     /locus_tag="L3M60_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012721120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBN6226806.1"
                     /translation="MSGADGEYFKKLEATIFYKEFALRSCISIIANALVLSEFQTFIN
                     SKSVKEKNYYLFNIEPNQNQNATEFWQEVITRLVYDNECLVIQENEQLFVAESFNVDK
                     YVFYEDVYKNITIRNYKLDRSFRESDVLYFKLNVESIKTVIDYLYNDYKDLLSSAMKG
                     YKKNNSEKGVLEIDTNYPQTKEAQERLNDLMSNKFKRYFESDNAVLPLSNGLKYTENT
                     KASSIKDSRDIRAIIDDVMDFVCAAFHIPAGLIKGNVSGVEGITDNFLNFSVNPIARL
                     ITTEITRKMYGRDKFFEKSYVKVDTQKIANMGLEKISKASDLLFRIGVNSINDNLEML
                     GREKINESWADEHYVTKNYQSILNPDLKGGANNGNGEKSGIAGNKGQ"
     gene            5563..6192
                     /locus_tag="L3M60_002146"
     CDS             5563..6192
                     /locus_tag="L3M60_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBN6226807.1"
                     /translation="MEKRAVSLEIRDSNLESRKISGYAAIFNDDYTKLQDRWGDTFYE
                     KISRGAFLKTLADNTRDKFMLINHDWNKVIGRTNSNLILEEDERGLRFELDIPATTDG
                     NDLLENVRLGLIKGCSFGFNIVNSKTRFDDDWTYYRDITEVELFEITATPIPAYNDTE
                     INCRSDISIKELRESQKEKVKQNDNKDNIEKRNVDLVSAFFNAFNRGGK"
     gene            6196..7362
                     /locus_tag="L3M60_002147"
     CDS             6196..7362
                     /locus_tag="L3M60_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HBN6226808.1"
                     /translation="MAIKNLDEATNVELRTNLMEAIKSNDEEKITTSFLRMAEDIQSN
                     LLKEARSIANIESADRAILDKRGMPQLTSEERAYYSQVIEKRGFTDLEITMPRTIFDR
                     VFDDLEQNHPLLSAITFENTTGVTEWVIRKTGTEAAWWGQLTDPIKKELEGGFAKINT
                     VAYKLSSYLPVAKSMLDLGAEWLDRYVRVVLTESISLGLEMGIVAGTGKDQPIGMMKD
                     LKGSVVEGVYPDKTAKPLADFKPNTLGTEIMAPLTNGGKRTVTSVIMLVNPVDYWAKI
                     FGATTMLTSNGAYVYGVLPIPGNIIQTVAVPKNKLIVGVAKDYFMGIGSAGKIEYSDE
                     YKFLEDVRVYLAKQYATGTPKDNFSFLVFDIENLDTTLATEINSNTKTSATRAK"
     gene            7396..7665
                     /locus_tag="L3M60_002148"
     CDS             7396..7665
                     /locus_tag="L3M60_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226809.1"
                     /translation="MVEEVKEYLNITWIEEDAKIEKMVVRGINYLENEIAGIALDFKD
                     NFNRELLFNYCRYVRNNAIEYFEENFSKELFRLQIKSAANEVTKI"
     gene            7662..8312
                     /locus_tag="L3M60_002149"
     CDS             7662..8312
                     /locus_tag="L3M60_002149"
                     /inference="COORDINATES: protein motif:HMM:NF017343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HBN6226810.1"
                     /translation="MKKIKFDNFNDGIIEFGEYKESYDIEGNALDEKEFIVNSKLFYS
                     NEYIREQDRLKFDNTGLKVSIKLKVPYMKLIKTSDVIKLNNEFYSIVKLDISNDKTSI
                     YLYLSELVDVLDKHISIFIKERKNVLEDVEMKFYKKVWACVEDLNSKELIENNSIKTS
                     TNKKFKIRYIEELDLSIKQNAITNFVIKYKDKFYNITQILNLEEENKILEITAESK"
     gene            8329..8655
                     /locus_tag="L3M60_002150"
     CDS             8329..8655
                     /locus_tag="L3M60_002150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226811.1"
                     /translation="MHSKIVTILEKLNIGIGFQEYDNFSNDEEYIIFFIENEYDANFS
                     DNENKSIVYSIKIEYWYKSLKNINKYISIKDIFKKENFIFLNSSDFIENEYYCKELKF
                     KYEEVI"
     gene            8656..9099
                     /locus_tag="L3M60_002151"
     CDS             8656..9099
                     /locus_tag="L3M60_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HBN6226812.1"
                     /translation="MSVEITTEGFDAVLSKIESMGKSGDKLLNEAVKAGGNVILQDAL
                     PRVSKRSGKLKDGLKVSGVKKKGGTKYVLVGITKEDNSKIFYGKFLEFGASAHKIPIK
                     KGKKKGRIINHPGVSPKPFLAPAYESKKDEAKNVMKEILKRGLGL"
     gene            9099..9434
                     /locus_tag="L3M60_002152"
     CDS             9099..9434
                     /locus_tag="L3M60_002152"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226813.1"
                     /translation="MININDLVIETLKPLKIPVSFQRFDGEEKTYITFFSYLEQNRYA
                     DDEVVGTEHYIQIDLFSKNKMSYINKEIERLLKKNNFIKRSIHEIKESDYSYHTIFRF
                     LFFAEKKED"
     gene            9438..10034
                     /locus_tag="L3M60_002153"
     CDS             9438..10034
                     /locus_tag="L3M60_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBN6226814.1"
                     /translation="MAIIGLRNYRLVKLLVDTESELEYDTKIMKLTGAKNIKIAPKVD
                     SAENYGDDQLLETASAMGAIEVEFEVASLTLEEKAFILGYKYKDGVLVEDKDFNPPNI
                     ALGFESPKSQGGNRMVWLTKGVCEPLEEEAKTKEDKVEFQSQKIKLKFMPRINDGRHK
                     ITADTDVENSPTAEEFFATAFLKTAAKPTAPAKTNLNK"
     gene            10049..10336
                     /locus_tag="L3M60_002154"
     CDS             10049..10336
                     /locus_tag="L3M60_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226815.1"
                     /translation="MEIKLTINEEEKIFKAPPIALKKLDNAFALSDKINDGLNMRELF
                     QELLDFTTDIYGEQFTKEELLNGFYPASDFMTKAFEDLSKVSGGFEEKVKN"
     gene            10426..10575
                     /locus_tag="L3M60_002155"
     CDS             10426..10575
                     /locus_tag="L3M60_002155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226816.1"
                     /translation="MFEKNWSMVDIDNMDIFYYLDILAYMKENKKDNNNPNENDVYID
                     QISWL"
     gene            10588..10803
                     /locus_tag="L3M60_002156"
     CDS             10588..10803
                     /locus_tag="L3M60_002156"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226817.1"
                     /translation="MCKYCEVEESEAAFKKGKTNRGELITCDCDIEMWIEKESNEYFL
                     NSECWCGYYSESFEPIKFCPFCGRKLI"
     gene            10826..11044
                     /locus_tag="L3M60_002157"
     CDS             10826..11044
                     /locus_tag="L3M60_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226818.1"
                     /translation="MCKYCEVKINTEPFNRHDVVIRGELLTNDSDIDMYIELKNEKYF
                     MTGASYDYPGISGKKPISFCPFCGRTLT"
     gene            11104..16305
                     /locus_tag="L3M60_002158"
     CDS             11104..16305
                     /locus_tag="L3M60_002158"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBN6226819.1"
                     /translation="MAENVGELVVRVAMENSSFQQGVNNLNRQMKLIRSEFKNSGAGV
                     KNFGTSLDGLKSKQQMLSSTIQQQTKVVEAYKNKLNESKTTLSNTAQKQVELKEKVNQ
                     AKSAYEQSKATLGENAEETKRLKSELDSLEQEYAKNEEKIRSNSAAVDNWSIRVNNAE
                     SRLSEMRSELDDTTERINQQENKWNQLATKMTEIGNKFSSVGKQMQDVGKKMTMSITA
                     PILAIGAAASKLGMDFEASMSNVQGLSGATADEMVQLEKAAREAGASTSKTAKDAADA
                     LGYMALAGWDAKTSMEALMPVLRLSEAGNLDLARTSDLVTDSMSSLGLSVKELPTYLD
                     QVAKTAASSNTNIDALMEAMIVSGGTFKNLNVPLSEANALLGILANRGLKGSEAGNSL
                     NSIMINLTSGAGQAGVAMKELGLSAFDSNGKFKGMANVLMELKEKTKDMTEEQRTMYL
                     SMIGGKTQISTLQALLSGVGEEYSELEKKVGNSTGALDKMAKTMQNNNKGSITALISA
                     VQELGLKIYDVLKPSIAQAVSWFQNLTNKLNEMKPSTVETIVKIAGLAAALGPVLLIG
                     GKIVSMIGGAIGAFGTFSAAMAVVTTGAAAATPAVGALAAILGALSSPAAVAIAGITA
                     LGISVATVAKDMSKDAIEPISRFGKSVSESTKEAVGAFMDLEEKTTISLNQLMWSGET
                     VSEKMKQSIVSNFTEMSNQIIAKLQESKEQGIQSLQEMFATSKNLSDKEKEELIKNTE
                     EAYSSKEEKIKKSNEKVNQIMTKASQEHRTLTKEESAEINQIKTDMLNTAVVTMSKSE
                     AEQAAIMERMKANHVDLSAKEAAEVVKNSIKKKDESIKAANEEYNERLKIAAQLRAEG
                     GAENEKLADKVVEEATRQKDESINKAKEMHQGVVAEAQKQAENQASKVDWTTGEIKSK
                     WQVLNDTVKSKMQEWDSIAGDWVEGLKEKISTGWANLKENTAILWDNIKLTVTTKWTE
                     IKESISLKIEEIKQSISEKWESIKLFFTTTWESIKLIFSNAWDAIKQQLEEKFAPFIE
                     RLKIIFDGIKEFFRSTWEVIKNIVLGAVLLILDLVTGNFDKLKTDAIQIWENIKQSLS
                     DAWESIKEVFNNALAAIGDLLSNMWESMKSTAITIWESITQTLSSIWENIKSTCSTAW
                     EGLKTICSNTWENIKSTLTNAWESMKTAVTTKLGEIKNNIVTTWNNVITFFKTLPAKL
                     KQAAIDMFTRLREGIDSKKQDAVNASQKIGTDVVNKIKEFPSKLLTAGQELVNGLVKG
                     ISGFAYKVVDEAKKLGQKAADAVRNALGIHSPSRVMIEIGKFVDEGLAQGIANNSDSV
                     KKATEKITKIIEEETKKATKACEADIKLFNKQIDELSKREKAALKKVKGNAKDALKEE
                     YAAKKEKIKEAIELRKEQKEKEVEQLKEIANSAKEQLNKELQDRQEFVKKVDDLTKAI
                     VDALKEKYKQEYEAKQASIKKELEALDRWKEESIDRINSVYDAKIKAIDNELEAFEKA
                     EKEKDRLEQDKEELNKIEEIKTAIKFEHDDTNKEQLQKELEKILAERKKRIEQQEIED
                     KKEALKKRKEELEEKKKDEIENINQIYESEKEYYNKRLEDAKKFYDERIKESKLQAEA
                     EKMIMDKNQKEIVALLNSYSDAYKNAGQTLGEKLLEGFKPAINEIKNLIDSITREINN
                     ARNEALSLNSDIRKGNSAANSNSGRREATYNNNSRTANYNVNVNSTDSNTAREIERTI
                     RSLAFSL"
     gene            16323..17189
                     /locus_tag="L3M60_002159"
     CDS             16323..17189
                     /locus_tag="L3M60_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015957549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HBN6226820.1"
                     /translation="MQKLICRNSKGQEIKLGNSRPFLLEKIENGANTSANISVSNNLG
                     DGVSIDDISIKERLLPVSAAIYAKNKEDLELKRAYLTSLFNPRLGEMEFIYTNNALTR
                     RVKGVVQDITFQTPVGLMQKFLIQLLTPSPFWMDEFVLKEEVALWVGDFEFTLEISNE
                     GIEMGHRVSNLICNVINNGDIECGMKIQFKALATVDNPSLFNVNTREYIKINRTLEAG
                     DLLEITTEFANKRIELVKSNYERINVFNWIDLDSEFLQLEVGDNLFRYDADVGIDNLE
                     MSIYYNPLYLGV"
BASE COUNT         7168 a         1948 c         3307 g         4769 t
ORIGIN      
        1 agtcttataa caaaaaaaca aaataaaaat ccagaatatt ttgaataata tagacgggtt
       61 ggaaatgcta caaaatacct aactagtccc aaaagctaaa tttaaggatt agttaggtat
      121 ttataacaaa aagtgcaaga ggtgcaaaaa tgaaaataaa aaaagagtat attgaaaaag
      181 tggaaaatat attaaaaatg ttaggagata aatatagaaa gttaagaata ttaaaaaaag
      241 aaatagcaat tcttaaacac aaagaaaact atagagaaat taatttcgaa gaactgggat
      301 ttaaagttaa aagaagtacg aaaggtatag atgatatagt tgtaacaata gaggatacta
      361 tctacaacaa agaaactgaa attgaaatta tagagcgaaa gctagagttt tataatattt
      421 acttaaaaga attatcagag cttgaacaga agatgataga attaatatat ttttataatt
      481 gggatgaaaa actacctatc acaaaaatag cttatgaatt aaattatgat agaactagtc
      541 tttataacaa aaaagctatt gctattaata aaatagctct aatgatatac ggtgatgaag
      601 ctttagcgta aacaatattt ttacaaaaaa aagactattt ttatacttca tttctaaaga
      661 aagcatgtta gaattgtatc atagaaaaaa gtgtacatta acaactgaat atccccttaa
      721 acctaaagtc taaacttggc tcgaagttta gacttttttt attgccgaaa aggaaggggg
      781 tgagtaaatg aactttgtag aacctattcg agatttagag aagctagaaa gcatgtgtgc
      841 ttatttagaa gaagctaaca tgagaaacaa tgtgctcttt agtatgggca tatatacagg
      901 acttagagta ggtgacatat taaagcttag aataaaagat gttcatgaca aacggtttat
      961 aactattaaa gaaagtaaga caggtaaaac caagcctatt gaagttaatc ctattctaaa
     1021 aagaatacta aaagaatata caaaagataa gaatcagaat gagtttttaa ttaagagtag
     1081 agaacagact aataaagcta taagtagagt aatggcttat aagataatac gagaagtagg
     1141 cgaaatgttt ggcatagata atctaggcac acatacaatg cgtaaaactt ttggttatca
     1201 ttactataaa aggactcatg atgttgtgac cttacagaag ctttttaatc atagttcagc
     1261 gacgataacc ttgcgttata ttggaattga acaggatgat ttaaacaaag catataggaa
     1321 tattaaatac ttttagctta agaatttaac ataaaaaggt cacgttaaac tggataaaat
     1381 atagtctaat aataaagtct tgaatgtatt gttagtacta aaatgtaagg ctgtttaata
     1441 atacttgaat gagtttaaca gaatatacct tatgttaaac tcgactagga aattaaagga
     1501 gtatcactat gaataaatat gataggttta aatgtaataa ttgcggaaga aagattgcag
     1561 taagtaataa agagttaagg actaaagtta ttgataatac tactgttaca tacttcatgt
     1621 gtcgcagatg ctttgagaag tttattgtat catgcgagga tgattatatt aaacataaga
     1681 aagaagaata taagaagctt aaagatgatg aggttaagct attgcaagac atgaaggagt
     1741 atagtgatgg tctaggagag ttgtatatca atgagttata agcatataac actagataga
     1801 ttaaaagaat taattttaat tgatagactt gatatattct atagaagcac ggattggcgt
     1861 aaattaagaa aaaaagctat tgaaagagat aattacgaat gccaagtttg taagaaaaaa
     1921 aagaaggtat ctaaggcgga atgtgtacat catattagag aaataaaaga gtatccagaa
     1981 ggagctttga agcttaataa cctaacaagc ttgtgcaacc tttgtcacaa cgaaattcac
     2041 gaaaaaatac taaaaattaa tcaagaaaaa aagaaaaaat tttttagtga tgaacgctgg
     2101 tagatagccc ccctattgaa aaaactggct ttcagagcga aaatttgtga accgaggtag
     2161 gtacatggct ccgcaaggag acgtcaattt aacaaaaggt gtggggggtg ggggtcttgc
     2221 aaccaaaatt atggttgcgg gtgcagaaaa aaaacataaa aatgaggtgg tgaaaatgga
     2281 tgataatgag gaattagaaa ttaggcgaga cgtacagaat gactacgttt taggcatgac
     2341 ttacaaagaa atgtcagcca agtataacat acccgaaaac actttgaaaa gttggagaaa
     2401 aagagggggt tggactcgca actgcacccc aaaaaagggt tgcgtgaaaa atgggttgca
     2461 agagtcggga aataatttaa aaaactctat aagaaaagat ttattaagac aaataaaaga
     2521 gaataacatg acagaagaat acttcaaaga cttagttgaa gattatttaa aaatgtggga
     2581 tataaaaaat gaattaatag aagatgtaaa aactagaggt gttcaagtca aatataaaaa
     2641 tggtcaaaat caatggggct ataaaaaaaa cgatagtgta tcagagctta ttaagtataa
     2701 cgctcaaatg ttaagtttac ttgataaact aggtataaaa gcgacaccaa aagttttggg
     2761 tgatgatgat attgaattat aagtacaata aatacataga tgaatatttc aacttagttg
     2821 ataaaggcga aattgttcta tgtaaagaaa tggaacttgc agttaagaaa ataaaaaaag
     2881 atttaaaaaa acctaatatc tatatcgacc atgaaaaagt tgaaaaagct atcgaataca
     2941 tggaaaagta tttctataaa atgtatgctt gggaaaaatt tgttgttgct ttagtagttg
     3001 gctgctttga aggcgacgaa cttttatatg atgaaatttt tatcatgatg ggcaggggaa
     3061 atggaaaaaa tggttttata tctcaattag ctaattattt gcaaacacac ttacacggtg
     3121 taaaaaatta tggtattgat attgttgcaa tgtcagaaga acaagcaaaa acaagttttg
     3181 atgatgttta taacatgtta tctgaacatc ctgctatatg caaaaagttt tttaacagaa
     3241 ctaaagaagt aataacattt aaaaaaacta acagctattt aaagttccgc acaaataatg
     3301 ctaagtcaaa ggatggatta agacccggct gcatcatttt tgatgaggtt catgcatacg
     3361 aaaattatga taatataaaa gtttttacct ctgcacttgg aaaagtaaaa aatcctaggg
     3421 ttttttacat tacaactgat ggaaatgtaa gaggtggggt attagacgat ttcaaagagg
     3481 aatcaaaaca aattttaaat gaagaaatta aaaactctaa aatgctgcca cttattttta
     3541 agcttgatag tatagaagaa atggaaaata ttgatttact ggaaaaagca aacccgagca
     3601 taaagtataa ttcagactta aggacacaaa ttttaaaaga cattgacaaa gctaaaaata
     3661 gaccgcagat gatgatagaa ttactaacta aaagatttaa tttgccagcg caaaatttag
     3721 cgactgttgt tgctgaatgg gatgacatag ttgcaacaaa cgaagaatta ccagacctaa
     3781 aaggcttttc gtgcattgga ggattagatt tttctagtat aagagatttt acaagcgtgg
     3841 tattgttatt taaaaaagat aaaaaacgat atatcatgca tcatactttt atatgttata
     3901 agtctttaga aataacaaat tttaaatttg atattgaatt agcaaagcaa aaaggtcttt
     3961 gtacaataat ttatgatgaa acgataaaag cagagtatat tgctgattgg ttcattgaaa
     4021 aagcggagga ttataatata ttaaaaatct gctgtgactc atatagagct gcacatgtaa
     4081 aagaagaatt tgctaacaaa ggtttaccaa tcgaagaggt tagaaatggt tcaataactc
     4141 atgcgaaaat agcccctctt gtagaacaaa tcttcgcaga gagaacaatt aaatggggcg
     4201 acgacatgat gatgcgttgg tatactaata atgtttatgt agatacagac aaaaaaggaa
     4261 ataaaacttt tttaaagata gagccaatca aaaggaaaac ggatggcttt tttgcatttt
     4321 tacatgcttt atcaaaagat ggtgaactga tgcaagaatc aacgattaag ttttatgatt
     4381 gttatacata ttaaaaataa tgaggtggtg aattgaattt taaaaatttt ataactaatt
     4441 ttttatttaa agataaaatg tctggagcag atggagagta ttttaaaaaa ctagaagcta
     4501 cgatatttta taaagaattt gcattaaggt catgtatttc tattattgca aatgctttag
     4561 ttttaagtga atttcagacg tttataaatt caaagtctgt taaggaaaaa aattattact
     4621 tgtttaatat agaacctaat caaaatcaaa acgcaacaga attttggcaa gaagtaatta
     4681 caagacttgt gtatgacaat gaatgtctag ttatacaaga aaacgaacaa cttttcgtag
     4741 cagaaagttt taatgtagat aagtatgttt tttatgaaga tgtatacaaa aatattacaa
     4801 ttagaaacta taaattagat agaagcttta gagagtcaga cgttttatat tttaaattaa
     4861 atgttgaaag cataaaaact gtaatagatt atctttataa tgattataaa gatttattaa
     4921 gtagcgccat gaagggttat aaaaaaaata atagcgaaaa gggcgtactt gaaatagaca
     4981 caaattaccc acagacaaaa gaagcacaag aacgacttaa tgatttaatg agtaataagt
     5041 ttaaacgtta ttttgaaagt gataatgctg ttttaccgct ttcgaatgga ttaaaatata
     5101 cagaaaatac aaaagcttca agtataaaag atagcagaga tataagagcg attattgatg
     5161 atgttatgga ctttgtatgc gctgcttttc atattcctgc tgggcttata aaaggtaatg
     5221 tttctggcgt tgaaggtata acagataatt ttttaaactt ttccgtcaat ccaatcgctc
     5281 gacttataac aactgaaata actagaaaaa tgtatggtag agataaattt tttgaaaaga
     5341 gttatgtaaa agtagataca caaaaaattg ctaacatggg acttgaaaaa atatcaaaag
     5401 caagtgactt actgtttaga ataggtgtaa attctataaa tgacaattta gaaatgcttg
     5461 gtcgagaaaa aataaatgag tcttgggctg atgaacatta tgtaactaaa aactatcagt
     5521 cgattttaaa tccggatttg aaaggagggg ctaacaatgg aaatggagaa aagagcggta
     5581 tcgctggaaa taagggacag taatttagaa agtcgtaaga taagtggtta tgctgctatt
     5641 tttaatgatg attatactaa attgcaagac agatggggcg atacttttta cgaaaagatt
     5701 tcaagaggtg catttttaaa aactttagca gataacacta gggataagtt tatgcttata
     5761 aatcatgact ggaataaagt tataggtaga actaactcaa atctaatttt agaagaagat
     5821 gaacgtgggt taaggtttga actagatata ccagctacga cggacgggaa tgatttgctt
     5881 gaaaatgtaa gacttggact aattaaaggg tgttcttttg gttttaatat tgtaaattca
     5941 aaaactcgct ttgatgatga ttggacatat tacagagata taacagaagt tgaactattt
     6001 gaaattacag caacccctat tcctgcttat aatgatactg aaataaattg tagaagtgat
     6061 atatctataa aagaacttag agaatctcaa aaagaaaaag taaaacaaaa tgataataaa
     6121 gataatatag aaaaaaggaa tgtcgactta gtatcggctt tttttaatgc atttaatcga
     6181 ggaggaaaat aaaaaatggc aattaaaaat ttagatgagg caactaatgt tgaactaaga
     6241 acgaatttaa tggaagcgat aaagagtaat gacgaagaaa aaataacaac atcattttta
     6301 agaatggcgg aagatataca aagtaattta ctaaaagaag cacgttctat tgctaatata
     6361 gaatcagctg acagggcgat acttgacaag aggggtatgc cacaattaac aagcgaggaa
     6421 agagcatatt atagccaagt gatagaaaaa agaggattta ctgatttaga gataactatg
     6481 ccgcgcacta tatttgatag agtgtttgat gacttagaac aaaaccaccc cctacttagt
     6541 gcaataactt ttgaaaatac gacaggcgtt acggagtggg taataagaaa aacgggaacg
     6601 gaggcagctt ggtgggggca attaactgac cctattaaaa aagagttaga aggtggattt
     6661 gcaaaaatta atactgttgc ttataaatta tcttcttatc ttccagttgc aaaatctatg
     6721 cttgacctag gtgctgaatg gttagacaga tatgtaagag tagttttgac tgaatctata
     6781 tcattggggc ttgaaatggg aatagtagca ggaacaggaa aagaccaacc tattgggatg
     6841 atgaaggact taaaaggctc tgttgttgaa ggtgtttatc cagataaaac cgcaaaacca
     6901 ttagctgatt ttaaaccaaa tacattagga actgaaatca tggcaccact tacaaatggt
     6961 ggtaaaagga cagtcacaag cgttataatg cttgtaaatc cggttgatta ttgggctaaa
     7021 atcttcggtg caacgactat gctaacttca aatggcgctt atgtatatgg agttctgcct
     7081 ataccaggaa atataattca gactgttgct gttccaaaaa ataaattaat cgtaggagtt
     7141 gctaaagatt attttatggg aattgggtca gctggtaaaa tagagtattc agatgaatat
     7201 aagttcttag aagatgtaag agtctatctt gctaagcagt acgcaactgg aacaccaaag
     7261 gataattttt cttttttagt atttgatata gaaaacttag acacaacttt agcaacagaa
     7321 attaattcaa acacaaaaac gtcagctact agagcaaaat aggtgaaaaa tatggaaaat
     7381 gaaaatatta aaagtatagt tgaagaagta aaagaatatc ttaatataac ttggatagaa
     7441 gaagatgcaa aaatagaaaa aatggttgta agagggatta attacttaga aaatgaaata
     7501 gccggaatag cattagattt taaagataat tttaatagag agttgttatt taactattgc
     7561 agatatgtaa gaaacaatgc tattgaatat ttcgaagaaa attttagtaa agagcttttt
     7621 agacttcaaa ttaaaagcgc agctaatgag gtaacaaaga tatgaaaaaa ataaaatttg
     7681 ataatttcaa tgatggtatt attgaatttg gagaatataa agaaagctac gatatagaag
     7741 gtaatgcact agatgaaaaa gagtttattg taaatagtaa actcttttat tctaatgaat
     7801 atataagaga gcaggacaga cttaaatttg ataatacagg gttaaaagta tctattaaat
     7861 taaaagttcc ttatatgaaa ttaattaaaa caagtgatgt tataaaacta aataatgaat
     7921 tttacagcat agtaaagcta gatattagta atgacaaaac tagcatatat ttatatttat
     7981 ctgaattagt tgacgtacta gataagcata tttctatttt tattaaagaa agaaaaaatg
     8041 ttcttgaaga tgtagaaatg aaattttata aaaaggtttg ggcttgtgtt gaagatttaa
     8101 acagcaaaga attgatagaa aataattcaa ttaaaacttc tacaaataaa aagtttaaaa
     8161 ttagatatat tgaagaatta gatttaagta taaaacaaaa tgctataact aattttgtta
     8221 ttaaatacaa agataaattt tacaatataa ctcaaatctt aaatttagaa gaagaaaata
     8281 aaatacttga aataacagca gagtctaagt aaaaaaatag gtggtagtat gcattcaaaa
     8341 atagttacta tacttgaaaa attaaatata ggaattggct ttcaagaata tgacaatttt
     8401 tcaaatgatg aagaatatat aatatttttt atagaaaatg aatatgatgc aaatttttct
     8461 gataatgaaa ataaaagtat tgtatattca ataaagatag agtattggta taagagctta
     8521 aagaatataa acaaatatat tagtataaaa gatatattta aaaaagaaaa ttttatcttt
     8581 ttaaattcta gtgattttat agaaaatgaa tattattgta aagaattaaa attcaaatat
     8641 gaggaagtta tttaaatgag tgtagagata acaacagaag gttttgacgc tgttttatct
     8701 aaaatagaga gcatgggcaa atcgggcgat aaacttctaa atgaagctgt aaaagctggc
     8761 ggcaatgtta ttttacaaga tgcattacca agggtcagta aaagaagcgg aaagttaaaa
     8821 gatggtttaa aagtaagtgg agttaagaaa aaaggtggta ctaagtatgt tttagttggc
     8881 attacgaaag aggataattc aaaaattttt tatggaaagt ttttagaatt tggagcatct
     8941 gcgcataaaa taccaattaa aaaaggtaag aaaaaaggaa gaataataaa ccatccaggt
     9001 gttagtccta aaccgttttt agcacccgca tacgagtcaa aaaaagatga agctaaaaat
     9061 gtaatgaaag aaatattaaa aagaggatta gggttataat gattaatata aatgatttag
     9121 taatagaaac attaaaacct ttaaaaattc ctgtttcatt tcaaaggttt gatggagaag
     9181 aaaaaacata tataactttt tttagttatt tagaacaaaa ccgctatgct gatgatgaag
     9241 tagtaggaac agaacattat attcaaatag atttgtttag taaaaataaa atgagttata
     9301 taaataaaga aattgaaaga cttcttaaaa aaaataattt tataaaaaga agtatacacg
     9361 aaataaaaga atctgattat agttaccata ctatttttag attcttattt tttgcagaaa
     9421 aaaaggagga ttaagaaatg gctattatag gattaagaaa ctatagactt gttaaattgc
     9481 ttgttgatac agaaagcgaa cttgaatatg acactaaaat aatgaaatta acaggtgcta
     9541 agaatataaa aatagcacca aaggttgata gtgcggaaaa ttatggtgat gaccaacttt
     9601 tagaaactgc ttcagcaatg ggagctatag aagtagaatt tgaggttgct agtttaacat
     9661 tagaagaaaa agcttttatt ttaggttaca aatacaaaga tggcgttttg gttgaagata
     9721 aagattttaa tccgccaaac attgcgcttg gctttgaatc tcctaaatcg caaggtggaa
     9781 atagaatggt ttggttaact aaaggtgtgt gtgaaccact tgaagaagaa gcaaaaacaa
     9841 aagaagataa agttgaattt caatctcaaa aaattaagct taagtttatg ccacgtataa
     9901 atgatggtag acataaaata acagcagata cagatgttga aaactcacca actgcggaag
     9961 aattttttgc aacagctttt ttaaaaacag cagcaaagcc aacagcacca gcaaaaacaa
    10021 atttaaataa ataaggagaa aattaaaaat ggaaataaaa ttaactataa atgaagaaga
    10081 aaaaatattc aaagcacctc ctattgcact taaaaaatta gataacgctt ttgcactaag
    10141 cgataagatt aatgatggtc ttaatatgag agaattattt caagaattat tagatttcac
    10201 aacagatata tatggcgaac aatttacaaa agaagaattg ctaaatggat tttatcctgc
    10261 tagtgatttt atgacaaaag ctttcgaaga tttatcaaaa gtaagtggag gtttcgaaga
    10321 aaaagtaaaa aactaggtaa tggagaagat acagaaaata attctaataa agaaaatagg
    10381 tatctgtctc caagagattt tataaaagat ttatatagta tgtttttgtt tgaaaagaat
    10441 tggagtatgg tcgatataga caacatggat atattttatt atttagatat acttgcatat
    10501 atgaaagaga ataaaaaaga taataataat ccaaacgaaa atgacgttta tattgaccag
    10561 ataagctggt tataaggatg tgagtgtatg tgtaagtact gtgaagttga agaaagcgaa
    10621 gcagctttta aaaaaggtaa aacaaataga ggcgagttga taacttgtga ctgcgatata
    10681 gaaatgtgga ttgaaaaaga aagcaatgag tattttttaa attctgaatg ttggtgtggt
    10741 tactatagtg aaagttttga acctattaaa ttttgccctt tttgtggaag aaaattaatt
    10801 taaaaataat tgaaaggaca aaaaaatgtg caaatattgc gaagttaaaa ttaatacaga
    10861 accttttaat aggcatgatg tagttataag aggtgaatta ttaactaatg actctgatat
    10921 agatatgtat atagagctta aaaatgaaaa gtattttatg acaggagcga gttacgatta
    10981 tccggggatt agtggtaaaa aacctatcag tttttgccct ttctgcggga gaacattaac
    11041 ttaataattt gaaataaacc aagattttta tcttggttta ttttttgcaa gtgaggtgag
    11101 aaaatggcgg aaaatgtagg tgaattagtt gttcgggtcg ctatggaaaa tagttcattt
    11161 cagcaaggtg ttaataatct aaataggcaa atgaagttaa taagaagcga gtttaaaaat
    11221 tctggtgctg gagtgaaaaa ttttggaaca agtcttgatg gtttgaaatc taagcaacaa
    11281 atgttatcta gtactataca gcagcaaact aaagtagttg aagcatataa aaataaacta
    11341 aatgaaagta aaacaacttt aagcaacaca gctcaaaagc aagttgaact taaagaaaaa
    11401 gttaatcaag ctaagagtgc ttacgaacag agcaaagcga cgttaggtga aaatgcagaa
    11461 gaaacaaaaa ggttaaaatc tgaacttgat agcttagaac aagaatatgc taaaaatgaa
    11521 gaaaaaataa ggtctaactc ggcagcggtt gataattggt ctattcgagt taataatgca
    11581 gagtcgagac tttctgaaat gcgttcagaa ctagatgaca caacagaaag aataaatcaa
    11641 caggaaaata aatggaatca actagcaact aaaatgactg aaataggtaa taaattcagc
    11701 tcggttggaa aacaaatgca agacgtaggt aaaaaaatga ctatgagtat tactgctcct
    11761 attctagcaa tcggagcagc ggctagtaaa cttggaatgg attttgaagc atctatgtca
    11821 aatgtacaag gattgagtgg ggcaacagct gacgaaatgg ttcaacttga aaaagcagct
    11881 agagaagcag gggcttctac atcaaaaaca gctaaagacg cagcggatgc gcttggatat
    11941 atggctttgg ctggatggga tgcaaaaaca tcaatggagg ctttaatgcc tgttttgcga
    12001 ttgtcggaag ctggaaacct tgatttggca agaacctcgg atttagtaac cgactctatg
    12061 tcatcacttg gacttagtgt aaaagagtta ccaacttact tagaccaagt tgctaaaact
    12121 gccgcaagtt caaacactaa cattgacgca cttatggaag ctatgattgt gtctggaggt
    12181 acttttaaaa atttaaacgt acctctttcg gaagctaatg cactgcttgg aatacttgca
    12241 aacagaggac taaaaggcag cgaggcagga aactctctaa attcaattat gataaatttg
    12301 acaagtggag cgggtcaagc tggtgtagct atgaaagaac taggattaag tgcttttgat
    12361 tctaatggta aattcaaagg tatggctaat gttttgatgg aacttaaaga aaaaacaaaa
    12421 gacatgacag aagaacaacg aacaatgtat ctatccatga taggcgggaa aacacaaata
    12481 tctactttgc aagccttgct atctggagtt ggagaagagt attcagaact cgaaaaaaaa
    12541 gttggaaata gtacaggcgc attagataaa atggctaaga ctatgcaaaa taataataaa
    12601 ggttctataa cggctttaat atcagctgta caggaactag gacttaaaat atatgatgta
    12661 ctaaaacctt ctatagcgca agcggttagc tggtttcaaa atcttacaaa taaattaaat
    12721 gaaatgaagc ctagcacggt agaaactata gttaaaatag caggattagc agcagcgctc
    12781 ggacctgttc ttttaattgg tggaaaaata gttagtatga ttggcggagc tataggggct
    12841 tttggtactt tttcagcagc aatggcagtc gtaacgacag gagcagccgc agcaactcca
    12901 gctgttggtg ctttagctgc aattttaggt gcattatcaa gccccgcagc tgtagctata
    12961 gctggaatta ctgcgctagg catatcggtt gcaacagtcg ctaaagacat gtcaaaggat
    13021 gctatagagc caattagccg ttttggtaaa agtgtatcag aatctacaaa agaagctgta
    13081 ggcgctttta tggatttaga agaaaaaacg acgatatctt taaatcaatt aatgtggagt
    13141 ggtgagactg tttcagagaa aatgaaacaa tcaatagtat ctaattttac agaaatgtca
    13201 aatcaaatca ttgctaaact tcaagagagt aaagagcaag gtattcagtc tttgcaagaa
    13261 atgtttgcaa cttctaaaaa tttaagtgac aaagaaaaag aagagttaat aaaaaatact
    13321 gaagaagcgt attcaagcaa agaagaaaag ataaaaaaga gcaatgaaaa agtaaatcag
    13381 ataatgacta aagctagtca agaacacaga acattaacaa aagaagaatc agcagagata
    13441 aatcaaataa aaacagatat gctaaatacc gcagttgtta caatgagtaa aagcgaagca
    13501 gagcaagctg caataatgga aagaatgaaa gcaaatcatg ttgatttgtc agctaaagaa
    13561 gctgcggaag tagttaaaaa ctcaataaag aaaaaagacg aaagtataaa agcagctaat
    13621 gaagaatata acgaaagatt aaaaatagcc gcacagctta gagcagaggg tggagcagaa
    13681 aatgaaaagt tagctgataa agtagtagaa gaagcaacaa ggcaaaaaga cgaatctata
    13741 aataaagcta aagaaatgca ccagggagtc gttgcagagg cgcagaaaca agcagaaaac
    13801 caagcaagta aagtcgattg gacaactgga gaaataaaat caaaatggca agtattaaac
    13861 gatacagtaa aaagcaaaat gcaagaatgg gacagtatag ctggcgactg ggtagaaggc
    13921 ttgaaagaaa aaatatctac tggatgggct aatttgaagg aaaatacagc cattttatgg
    13981 gataacataa agcttactgt aacaactaaa tggacagaga taaaagagtc tataagttta
    14041 aaaatagaag aaataaaaca aagtattagc gaaaaatggg aatctataaa attgttcttt
    14101 acaacaacat gggaaagtat aaaacttatt tttagtaatg cttgggatgc tataaaacaa
    14161 caattagaag agaaattcgc acctttcatc gaaagattaa aaatcatatt tgatggtata
    14221 aaagagtttt ttagaagtac ttgggaagtt ataaaaaata tagtacttgg tgctgtgtta
    14281 ttaattttgg atttagtaac agggaatttt gataagttaa aaacagatgc aatacaaatt
    14341 tgggaaaaca taaaacaaag cttatcggat gcatgggaat ctataaaaga agtgtttaac
    14401 aatgcattag ccgcaatagg tgatttactt tctaatatgt gggaaagtat gaaaagtact
    14461 gcaataacta tttgggagtc tataacgcaa actctaagca gcatatggga aaatataaag
    14521 tcgacatgtt cgactgcttg ggaagggcta aaaacaattt gctctaatac ttgggaaaat
    14581 ataaagtcaa ctcttacaaa tgcgtgggaa agcatgaaaa cagctgtaac aactaagtta
    14641 ggagaaatta aaaataatat tgtgacaact tggaataatg tcattacatt ttttaaaaca
    14701 cttccagcta aattaaaaca agcagctatc gacatgttta caagactaag agaaggtata
    14761 gactcaaaaa aacaagatgc tgttaatgcg tcacaaaaaa ttggaactga tgttgtaaat
    14821 aaaataaaag aatttccaag taaattacta acagctggtc aagaattagt aaacggttta
    14881 gtaaaaggaa tatcaggctt tgcttataaa gtagtagacg aagctaaaaa gctagggcaa
    14941 aaggctgctg atgcagttag aaatgcgctt ggaatacatt ctccatcacg tgtcatgatt
    15001 gaaattggta aatttgttga tgaaggttta gcacaaggta ttgctaataa tagcgactcg
    15061 gtcaaaaaag caactgaaaa gataacaaag attatagaag aagaaacaaa gaaagctaca
    15121 aaagcatgtg aagcagatat aaaactattt aataaacaaa tagacgaatt atctaaacga
    15181 gaaaaagcag cacttaaaaa agtaaaaggt aatgctaaag acgcattgaa agaagaatac
    15241 gcagctaaaa aagaaaaaat aaaagaagct atagaactaa gaaaagaaca aaaagaaaaa
    15301 gaagttgaac agttaaaaga gatagcaaat tcagctaaag agcagttgaa taaagaattg
    15361 caagacagac aagaatttgt aaaaaaagtt gatgatttaa caaaagcgat agttgatgca
    15421 ctaaaagaaa aatataaaca agaatatgaa gcaaaacaag caagtataaa aaaagagtta
    15481 gaagcattag atagatggaa ggaagaaagt atagatagaa ttaatagtgt ctatgatgcg
    15541 aaaattaaag ctattgacaa cgaattagaa gcttttgaaa aagctgaaaa ggaaaaagat
    15601 agacttgaac aagacaaaga agaattaaac aaaatagaag aaattaaaac agctataaag
    15661 tttgaacatg atgacactaa caaagagcag ttgcaaaaag aattagaaaa gattttagct
    15721 gaaagaaaga aaagaataga gcagcaagaa atagaagata aaaaagaagc tttaaaaaag
    15781 agaaaagaag aattggaaga aaagaaaaaa gacgaaatag aaaatataaa tcaaatctat
    15841 gaaagtgaaa aagaatatta caataaaagg cttgaagatg ctaaaaagtt ctatgatgaa
    15901 agaataaaag agtctaaatt acaagcagaa gcggaaaaaa tgataatgga taaaaatcaa
    15961 aaagaaatag ttgcgttatt aaatagttat tcagatgcat ataaaaacgc aggtcaaacc
    16021 ttaggcgaaa aattacttga aggatttaag cctgctataa atgaaatcaa aaatctaatt
    16081 gatagtatta caagagaaat aaataatgct agaaatgaag cattgagttt aaatagcgat
    16141 attagaaaag gaaattcagc agctaattca aattcgggaa gaagagaagc aacttataat
    16201 aataattcaa gaacagcgaa ttataatgta aatgttaata gtacagactc aaatacagca
    16261 agagaaatcg aaagaacaat aagaagtcta gcatttagct tgtaaaaaaa ggggtgtgaa
    16321 acttgcaaaa attaatatgt agaaactcga aagggcaaga gattaaacta ggaaactcac
    16381 gccctttttt gttagaaaaa atagaaaacg gagcaaatac tagcgctaat atttcagttt
    16441 cgaacaatct aggtgatggt gtaagtatag atgatataag tataaaagag agattgcttc
    16501 ctgtttcagc ggctatttat gcaaaaaata aagaagattt agagttaaaa cgtgcatatt
    16561 taacctcttt gtttaatccc aggctcggag aaatggagtt tatatataca aataacgcat
    16621 taactagacg cgtcaaagga gttgtgcaag acataacatt tcaaacacca gttggattaa
    16681 tgcaaaagtt tcttatacag ctcttaacac caagtccgtt ttggatggat gagtttgttt
    16741 taaaagaaga agttgctttg tgggttggtg acttcgaatt tactttagag ataagtaatg
    16801 aaggaataga aatggggcat agagtaagta atttaatttg caatgtaata aataatggcg
    16861 atattgagtg cggtatgaaa atacagttta aagcacttgc aacagttgat aatcctagtc
    16921 tatttaatgt aaatacaaga gaatatatta agataaacag aacccttgaa gctggtgact
    16981 tattagaaat aacaacagaa tttgcaaata agagaataga gttagtaaaa tcaaactatg
    17041 aaagaataaa tgttttcaat tggattgact tggatagcga atttttacaa cttgaagttg
    17101 gagataattt atttagatat gacgcagatg ttggaattga taatttagaa atgtctatat
    17161 attataatcc gctttatttg ggggtgtaat at
//
LOCUS       DAFNNB010000078        16920 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0079, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000078 DAFNNB010000000
VERSION     DAFNNB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16920
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0079"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..534)
                     /locus_tag="L3M60_002160"
     CDS             complement(<1..534)
                     /locus_tag="L3M60_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226821.1"
                     /translation="MKKFTICIIMFAISALVLAGCTNDNKIKNEVDNMFNAIKTQDSD
                     AVDKYFPKLGLGNLIYEDKEGFKMYSKNMTWEISDIKNNKDKVTVDLKVNNTDMVSIL
                     KKNPPKLGAMMPNPTMKDIDSANKKEFNLTLELVKSGDNYVCEVNQESAMKLLNVITG
                     GGTDYLADYNKDYYKQLD"
     gene            complement(539..1438)
                     /locus_tag="L3M60_002161"
     CDS             complement(539..1438)
                     /locus_tag="L3M60_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6226822.1"
                     /translation="MKLLSNINNNIRLITQVAFTALTNGYVNGFLEGTIYGGESKRLC
                     VPGLNCYSCPGALGACPIGSLQAVLSSREYKFSFYVVGFLMAFGAFFGRFVCGWLCPF
                     GLVQDLLYKIPFPVKIKKVYGDKYLKYLKYLVLIIFVIILPVAIVNVAGGGDPWFCKW
                     ICPSGTFLGGIPLILGNENLRESIGFLFSWKLSILLLILLISIMIYRPFCRYICPLGA
                     IYSFFNPISIYRFKIDKDKCTNCTACQAKCKLDIPVYKDVNSLDCIRCGECIKACPHN
                     AITTTFSKDKKENKFCKKKYRGM"
     gene            complement(1449..1598)
                     /locus_tag="L3M60_002162"
     CDS             complement(1449..1598)
                     /locus_tag="L3M60_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226823.1"
                     /translation="MSNFIKDNKTGISIIVISFIFIVLGTYRGESNTVLTKAIMICLE
                     CIGIG"
     gene            complement(1591..2178)
                     /locus_tag="L3M60_002163"
     CDS             complement(1591..2178)
                     /locus_tag="L3M60_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HBN6226824.1"
                     /translation="MKRFFTLVLGCIFVLGVVSGCNKKTETNLVETNKNNSEWFNNLV
                     TEDVDGNKVTKDVFSSKELTLINTWTTWCGPCIGEMPELEALSKEYENNNSNVAIKGL
                     IVEVDGTDMKTGLSDKEKELVKDIMKKSGATYQQLTVSEGLKKTDFKRTIEFPTTYFV
                     DKKGNFVGEKVTGANSKEEWKKIIDERLKMVKDSE"
     gene            2601..3926
                     /gene="cdeA"
                     /locus_tag="L3M60_002164"
     CDS             2601..3926
                     /gene="cdeA"
                     /locus_tag="L3M60_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter CdeA"
                     /protein_id="HBN6226825.1"
                     /translation="MENLFTRKFTTFEFLKFVSPAIISMIFISLYTIIDGIFVSTLVG
                     SDALASINIVLPIINLVCGFGIMMATGGGAIVSIRMGENRQNEANSTFSFIVLFSLIV
                     GILFTVISYFFIKEISILLGATNKLLPYCITYGKVMILCTPFYILKFIFEYFARTDGN
                     SKFSLFLSVIGGVTNIILDYVFIKYFGMGLLGAAVATAIGIILTCVLGILYFLSSKST
                     LKLRKPKTDFRLIRDTMINGSSEMVTELSTGVTTFLFNIVALKLAGESGLAALTIVLY
                     AHFLMTSVYLGFAAGVSPLISYNFGAENSDKLKETFKHSLKFIFVSSLLVFIVALVFA
                     PFIVRVFVNPDNTVFKLALQGLKIFAFAFLFVGINIFASGFFTAFHNGKISAIISFSR
                     AFVFIIIGIIILPPMLNLTGLWLTVPFAEVLTIFISILFIKKYKGRYKY"
     gene            complement(4021..4815)
                     /locus_tag="L3M60_002165"
     CDS             complement(4021..4815)
                     /locus_tag="L3M60_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6226826.1"
                     /translation="MNELKPYIKFFNKNTKLYIIIFMIMGIVMNILPVFILKIFGEAS
                     KTNLSTIVNPILTYITILLFVYGVNIANKDFSGALSIRADRKSCIKAIVIDLIILSFV
                     ISILGIVLIFLSKLFIEIITGYNIELSVLLKRDMIWTSISALIEPSTSSISLTYLNYF
                     VQICLNEICIGFIGVLLGAFTYRVRKVTSITILIGLPILMVIYIVNFATKNMHKMLLY
                     LEKIIYSFQNPFILFGTKVGIIVICIIFIILLLRKAPIKEYANDLL"
     gene            complement(4816..5691)
                     /locus_tag="L3M60_002166"
     CDS             complement(4816..5691)
                     /locus_tag="L3M60_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226827.1"
                     /translation="MSSSIKIENLYHSFKKQNVFNNMSLNFEKNKIYGLLGKNGAGKT
                     TLLNIMVNQLIQNEGEIEILGKNPKEDIKVLEEVCIVREKEFYSPDFKVGQVFEFSSS
                     FYKNYDKGLEEELCKYFDLNTKKKYKQLSRGMKTILSNIIGICSNSAITIFDEPTIGL
                     DAVNRQEFYNIILDSYIKNPRTIIISTHLIDEIDDLLEHVIILNEGKIIIDEEIDVIK
                     QKAHYITGNKEELEKLECIKSIKPKKAFGNTVAYFYYGDFSENDEKILKNSNIDVGYI
                     GLQDMFVNMTKKEEL"
     gene            complement(5695..6063)
                     /locus_tag="L3M60_002167"
     CDS             complement(5695..6063)
                     /locus_tag="L3M60_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226828.1"
                     /translation="MKLVLNNEEPIFIQIARAIEDEILSNGIKEEEQVPSTTELSKLY
                     KINPATVLKGINILVDKNILYKKRGIGMFVSDGAKTIIKEARKENFKHNFIKNLLQEA
                     KKLEINREELVNIIINFKED"
     gene            complement(6529..7188)
                     /gene="deoC"
                     /locus_tag="L3M60_002168"
     CDS             complement(6529..7188)
                     /gene="deoC"
                     /locus_tag="L3M60_002168"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBN6226829.1"
                     /translation="MKHILQTVDHTMLKATTTLKDIKTLCDEAVRMNVASVCIPPSYV
                     KSATEYLNGRVNICTVIGFPLGYQTTKVKVFEAKDAIENGAVEIDMVVNISDIKNEEY
                     EKIENEIKEIKSVIGDKVLKVIIETCYLNEYEKIKMCEIVTNSGADFIKTSTGMGTNG
                     ATLEDIKLMKKHIGEGVKIKAAGGIKSISDAEKFIEAGAQRLGTSSICKILNGEKPME
                     Y"
     gene            complement(7215..8759)
                     /gene="murJ"
                     /locus_tag="L3M60_002169"
     CDS             complement(7215..8759)
                     /gene="murJ"
                     /locus_tag="L3M60_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBN6226830.1"
                     /translation="MSRVARAAFYLMIVTIISKILGMGRELVLSSIYGTGLYTESYLT
                     AMNIPNIIFAAIGTAIVTTFIPMYQDINSKKGEKEALKFLNNVLNIIVGICIIVAILG
                     VIFSKQLVSIFAIGFEGERFLLTVKFTRILIIGIIFIGITSVMSAFLQIKENFIVVGF
                     GSIPYNIVIIISIVLSTVLGPYVLPVGAVVAMVVQLFFYLFFVNKTKYKYLFYLDFKD
                     ESLIKLLALLSPVFIGVAVNQVNSLVDTTLASTLIKGSIPALTYADRLNGFVTGTFTA
                     SIVSVMYPTLSKLSAENNQKKFTISVKSSVNMIIISMIPISVASIVFAKPVVRIIFER
                     GAFDSRATQMTATALVFYAVGMTAFGLRDILGKVFYSLQDTKTPMVNGIVSVGVNILL
                     DLALIKPMAHGGLALATSSSSIACILLLFLNLKRKVGYFGQDKIIRAIFKSLVSSCVM
                     SFIAYFIYKFIFGILGLGTLNELISLTISVVIGFSVYTLLMILFKVEEVDMILNIAKR
                     KLHLKK"
     gene            complement(9054..9818)
                     /locus_tag="L3M60_002170"
     CDS             complement(9054..9818)
                     /locus_tag="L3M60_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226831.1"
                     /translation="MKNINKECLIKLLTVMVVLIQIIYLLWKKEIVYYIHPRMIPYLV
                     ISICILLFIALSYIMILTKPKHIYQYGSTKYIILSFLITTLLISYSGLNIDKEKTKVE
                     KYKGKEKYQDKIYEKEEFLDRTLEERIYDEKFDVFDTEIYDFLNLLYTKQSVLKGKEV
                     TYVVFVHYDDSLEKNQFIASRSIMVCCAADMISSGPIVNMNQPPKWKEGEWILLTGKI
                     EYKEQKNSSNKTAFLNYIDAKPIEKPLEEVVYPNFG"
     gene            complement(9959..11515)
                     /locus_tag="L3M60_002171"
     CDS             complement(9959..11515)
                     /locus_tag="L3M60_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBN6226832.1"
                     /translation="MSTRIDIVAGFLESGKTKFINEYLRNKQYDDKDNILLIVLEEGF
                     TEYSIPFSSKNYIKIERFKDINCLTKEYLKKCSNLYNYILIEYNGTWNIEEFLDIKLP
                     YSCYLGKIYCLVNADTFDVYFRNIDSMVEQIINSDEFYISGSNSIEKTEKITSYIRAL
                     QPSADIFYIDESGNYKKIFPLSLKLKNLIYRIIKFVIVILFFLLLLGVKIQIIKVQDI
                     NDFNRMFFGIILQAIPFLLIGIVLSSCIQFLVSEEKFIYFASRHKVLSYPIFLFMGCI
                     LPLCDCAMVPITEKFIQKGLKVSHAMTFFCISSSINPIVILATIYAFPENNKVIWIRI
                     LVSIMLSALVGISFYFVEKNKNFSPYKKNKIPLFNNIEYEITKITKKGKKFRFLALGI
                     HIGNEFLRIMMFIIIGAFISTIAQILKMNPAFSGYFTNDYAFLYMFFASVFLSICASS
                     NAFIAKGFTNSLPLMSVIPFMVFGPLLDIKNLIVMSSRFSKKFMTIFIIILLVCYIMY
                     FVFFNMEIKL"
     gene            complement(11516..12445)
                     /locus_tag="L3M60_002172"
     CDS             complement(11516..12445)
                     /locus_tag="L3M60_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein P47K"
                     /protein_id="HBN6226833.1"
                     /translation="MIKIDIISGFLGSGKTTFIKKLLSAYYKDKKVVLIENEYGEVGV
                     DGLLFKNENIQVRELYSGCICCSLNTDFTKSLKEVIELFKPDTVIVEPSGVGKLSEVI
                     QNCIPLVKQNLVSINHKFTIVDVMKYSVYLKNFKEFYDDQIKYCDAVLLSRVDVCDEI
                     KVREVISSIQNKYHKKYIVSLEQNLLDETIVNYIMSTKSVVHSENDMHCNCHHNHVVH
                     NADDVFSSWGIKLEKSYKYEYIKEILEQFNSGSFGNIIRAKGIFKCEDGLFYEFSFVP
                     DEIKVIPCNYISSGCICVVGFKPNKEKLERAFV"
     gene            complement(12501..13289)
                     /locus_tag="L3M60_002173"
     CDS             complement(12501..13289)
                     /locus_tag="L3M60_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HBN6226834.1"
                     /translation="MLSYGFMQRALFVGLCVSIAIPCVGLILVLRKFSMLGDALSHTA
                     LAGVCFGLVVGISPTIGAIMSCIIASFSVSIIGKRMKHNEEIAIAVILSLGVGISGIL
                     SNYIKSSVNINSFLFGSIISISGREFALVIALTILILIIFFKYYYEFMFYAFNEKEAQ
                     KHYVNTRKIDILFTILIGLTISISARVVGALIISSLLVLPVACGLILGKSYKSTLLIA
                     IFLSMLFVLSGLTISYYIGLSPGGAIVILGSITLMIILLYKKRV"
     gene            complement(13282..13926)
                     /locus_tag="L3M60_002174"
     CDS             complement(13282..13926)
                     /locus_tag="L3M60_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6226835.1"
                     /translation="MKNIVEIKDGAFSYTDCNVFKSVNVNINKNELVGVYGENGSGKS
                     TLFKIITKEVKLQSGEIFTFDSLTTSFLGQNDRVMAEGSPLTLMEFLLMYQHKEKSKL
                     FKNKKNVMMLLRKYNMEHYAKKQLKSLSGGQLQKSMILKSIMNHPDIILFDEPLNALD
                     EVNKISVMDMIMDLHNNGHTILLILHDYEQLKKIADKILICENHTIKCEVLNYA"
     gene            complement(13929..14831)
                     /locus_tag="L3M60_002175"
     CDS             complement(13929..14831)
                     /locus_tag="L3M60_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HBN6226836.1"
                     /translation="MKRFVLLFCCSLLLLTGCSFKDVKKTSSNKKLSVYTSFYPMYDF
                     AKKIGGTKVDVKNMTPAGIEPHDFEPTTNDIKGLSNADMFIYNGDNMEHWVNDVIQSL
                     DNRNILIVKASDGIDLMKGEKSSDSMDPHTWLSIRNAKKELEKIKNAFCKKDPDNATY
                     YEKNYYKYASEFDALDSQYKKELLPYKGRSIVVAHQAFGYLCKDYNLKQIAIEGLSPD
                     SEPSPARMAEIIKSAKKNKIKVIFYEELVNPKVAETIAKETGSETKVLNTLEELSDKK
                     IKEGEDYISVMKTNLQNLVYALSK"
     gene            14966..15397
                     /locus_tag="L3M60_002176"
     CDS             14966..15397
                     /locus_tag="L3M60_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBN6226837.1"
                     /translation="MVVLYMNNYLKKHNLKSTEKREAVIKVLENSNVPMTIESIHEKA
                     NTIVEMNLSTIYRTITTLCEKNITVLVAIHDGKSYYQLNKHRHEHVLICKECKDIIII
                     DECPLHELEHKLEDETGFDIIEHKLEFIGICPKCKRTNSRT"
     gene            complement(15872..16756)
                     /locus_tag="L3M60_002177"
     CDS             complement(15872..16756)
                     /locus_tag="L3M60_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6226838.1"
                     /translation="MDTKKENNYENYICVGSSNVSNAMKMYLKEIEEYKMLSAGEEVE
                     LAKEIINSSSVAKEKFINSNYRLVVSIAKRYKRDSIDMLDLIQAGNIGLIKAVEKYDY
                     KKGYKFSTYATWWIKQSITRYIDDCENTIRIPIHLHQRINFVKKKKQELLNVLLREPT
                     IDEIADACGLEVDKVLELLRRDKNVVSLDTPLKEDEDSSLVEFIPSDADFKDVVMHEV
                     EQHNLKEKIEELLTGLGEQEQQVLRMRFGIDDDDPKTLEQIGRVFGVTRERIRQIEAK
                     AIRKLRHPSKLKQLKHFY"
BASE COUNT         5839 a         2784 c         1747 g         6550 t
ORIGIN      
        1 atcaagttgt ttgtagtaat ctttattgta gtcagctaaa taatctgtgc cacctccagt
       61 aattacattt aacaatttca ttgcagattc ttgattaact tcacaaacat aattatcacc
      121 agatttaaca agctctaatg taagattaaa ttctttttta tttgcagaat caatatcttt
      181 catagttgga tttggcatca tagctcctag tttaggagga ttctttttta aaattgaaac
      241 catatcagta ttgttaactt ttaaatctac agtcacttta tccttattat tctttatatc
      301 tgaaatttcc catgtcatat ttttagaata cattttaaat ccctctttat cttcatatat
      361 aagattacct aatcctaatt ttgggaaata cttatctaca gcgtctgaat cttgtgtttt
      421 tattgcatta aacatattat ccacttcatt ttttatctta ttatcattgg tacatccagc
      481 taataccaat gccgatattg caaacattat aatacatata gtaaatttct tcattaaatt
      541 acatccccct atactttttt ttacaaaatt tattttcttt tttatctttt gaaaaagttg
      601 tagtaattgc gttgtgtgga caagctttta tacattcgcc acatcttata caatctaaac
      661 tatttacgtc tttatatact ggtatatcaa gcttacattt tgcttgacat gctgtgcaat
      721 tcgtacattt atctttatcg attttaaatc tatatattga aataggatta aaaaaactat
      781 aaattgctcc taaagggcat atatatctac aaaatggtct ataaatcatg attgatatca
      841 ataatatcaa tagaagaatg cttaacttcc aactgaataa aaatcctatt gattccctta
      901 aattctcatt tcctaaaata agtggtattc ctcccaaaaa ggtccctgat gggcaaatcc
      961 acttacaaaa ccaaggatct ccacctcctg ctacatttac aatagcaact ggcagaataa
     1021 ttacaaatat tattaagacc aggtacttta aatattttaa atatttatca ccataaactt
     1081 tttttatctt tactggaaat ggtattttat atagtaaatc ttgcacaagt ccaaatgggc
     1141 aaagccaacc acaaacaaat ctaccaaaaa aggctccaaa tgccattaaa aaaccaacaa
     1201 cataaaatga gaatttatac tctctgcttg ataaaactgc ttgtaatgaa cctattggac
     1261 atgctccaag cgcgcctgga catgaataac agttcaatcc aggtacacac aatcgtttac
     1321 tttctcctcc atatatagtt ccttctaaaa atccatttac atatccattt gtcaatgctg
     1381 taaatgcaac ttgagtaata agccttatat tattgtttat attagataat aacttcatac
     1441 cttttctcct aaccaatacc aatacactct agacaaatca taattgcttt agttaatact
     1501 gtatttgatt ctccacgata tgtacctaat acaataaata taaaactaat tacaataata
     1561 gaaatgcctg ttttgttatc tttaataaag ttactcacta tcttttacca tttttagcct
     1621 ttcatcaata atttttttcc attcttcctt gctatttgca cctgttacct tttcacccac
     1681 aaagtttcct tttttgtcca caaaatatgt tgttggaaac tctatagttc tcttaaaatc
     1741 agttttttta agaccttcag atacagtcaa ttgttggtaa gtagcacctg actttttcat
     1801 aatatctttg actaattctt tttctttatc tgaaagacca gttttcatat cagttccatc
     1861 aacttctaca atcaatccct ttatagctac atttgaatta ttattttcat attcttttga
     1921 taaagcttca agttctggca tttcacctat acatggccca caccaagtag tccaagtatt
     1981 aataagggta agttctttac ttgagaatac atcttttgtt accttatttc catcaacatc
     2041 ctctgtcacc aaattattaa accactcact attattcttg tttgtttcta ccagattggt
     2101 ttctgttttt ttgttacaac cagaaaccac acctaaaaca aagatacacc ctaaaactaa
     2161 cgtaaaaaac cttttcatac ctcacactcc tataatttat atagtatata aaatcgtcgt
     2221 ttgaaataaa aataacttat cttctataat taaagtcatt ttatttaata aataagagtc
     2281 aattaaatca aattgtataa aagatataca gttaaaatat atttcttttc agacaaaatc
     2341 ttatatctaa ataatagatt gtaattctta agattatata catatatttc taaagatttt
     2401 cttaagaaaa atttagattt cactaataca atatacgtac ttttaataat ataagcttat
     2461 taattagcat tatactaatt catttcacta tgattttttg ttgattttaa gctgtttacc
     2521 atatgaataa agtttttctt gactatatag ttactatata gcttattata tatagagagt
     2581 aacaaaagga ggtaaaattt atggaaaatt tatttacaag aaaattcact acttttgaat
     2641 ttctaaaatt tgtttctcct gcaattatat ccatgatatt tatatctttg tacacaataa
     2701 tagatggcat ctttgtatcg acattagttg gttcagatgc tcttgctagt ataaatattg
     2761 tactacctat aattaatctt gtttgtggat ttggaataat gatggcaact ggtggaggtg
     2821 ctatcgtttc tatacgtatg ggtgaaaata gacagaatga agccaattct acattttctt
     2881 ttatagtttt gttttcattg attgttggga ttttattcac agtaatctca tatttcttta
     2941 tcaaagaaat atctattttg cttggagcaa caaataagtt actaccatat tgtataactt
     3001 atggaaaagt tatgatttta tgtacaccat tttatatttt aaaatttata tttgaatact
     3061 ttgcaagaac tgatggaaat tctaaattta gtttatttct atcagtcatt ggtggtgtaa
     3121 caaatataat attagattat gtatttatta aatattttgg aatgggtctt ttaggagctg
     3181 cagttgcaac tgctataggt attattttaa cttgtgtttt aggcattctt tacttcttat
     3241 cgagtaaatc tacactaaaa ttaagaaaac caaaaaccga ttttagactt ataagagata
     3301 ctatgataaa cggttcttct gagatggtca cagaactgtc tacaggagtt acaacattct
     3361 tatttaacat agtagcctta aaattagcag gagaaagtgg gcttgctgct cttaccatag
     3421 tattgtatgc tcatttttta atgacatcag tctatctagg atttgctgct ggagtgtctc
     3481 cattaataag ctataatttt ggtgctgaaa atagtgataa attaaaagag acatttaaac
     3541 attctctaaa atttatattt gtttcttccc ttttagtgtt tattgttgct ttagtatttg
     3601 caccatttat tgttagggtc tttgtaaatc cagataacac agtatttaag ctagccttgc
     3661 aaggattaaa aatatttgca tttgcttttt tatttgttgg tataaatata tttgcatcag
     3721 gcttttttac agcatttcac aatggaaaaa tttcagctat tatatctttt agtcgtgcct
     3781 ttgtttttat aatcatagga attataattc ttcctcctat gttgaacctg actggattat
     3841 ggcttacagt tccatttgct gaagtcctaa ctatatttat atctattcta tttataaaaa
     3901 aatataaagg tagatataag tactaaaaat taatatttaa aatcaacatt tattaaaact
     3961 aatgaacctg ctttaaaata caaatttata aatttgattt ttaagcaggt tttattttag
     4021 ttataataag tcatttgcat actctttaat tggtgctttt ctaagtaata aaattataaa
     4081 tattatgcaa ataactataa ttcctacttt tgttccaaat aaaataaacg gattctgaaa
     4141 tgaataaata attttttcta aatacaatag cattttatgc atatttttag ttgcaaaatt
     4201 aactatataa attaccatta atatcggtaa tcctatcaat atagtaatac tagtaacttt
     4261 tctaactcta tatgtaaaag ctccaagtaa tactcctata aagcctatac atatttcatt
     4321 taaacatatt tgtacaaaat aatttaaata agttagtgat atacttgatg ttgaaggctc
     4381 tatcagtgct gatatagatg tccaaatcat atctctcttt agtaaaactg acagttctat
     4441 attgtaacct gttataattt ctataaataa cttcgataaa aaaatcaaaa ctatacctaa
     4501 aatagaaatc acaaagctta aaataattaa atctatgaca atagctttta tacaactttt
     4561 tctatctgct cttatactta aagctcctga aaagtcttta tttgctatat ttactccata
     4621 cacaaatagt agtatagtta tatatgttaa aataggattc acaatcgtgg ataaattagt
     4681 tttagatgct tctccaaaaa tcttgaggat aaacactggt aaaatattca taacaattcc
     4741 cataatcata aatataatta tatacaattt agtattttta ttaaaaaatt ttatatatgg
     4801 ctttaattca ttcaattaca attcctcctt cttagtcatg ttaacaaaca tatcttgtag
     4861 ccctatgtat cctacatcaa tatttgaatt tttaagtatt ttttcatcat tttcactaaa
     4921 atcaccatag taaaaataag caactgtatt tccaaatgcc tttttgggct ttatactttt
     4981 tatacattct aatttttcta actcttcttt atttccagta atataatgag ctttttgttt
     5041 tatgacatca atttcttcat ctataataat ttttccctca ttcaatatta ttacatgctc
     5101 cagtaaatca tctatttcat caatcaaatg agttgatatt attattgtcc ttggattttt
     5161 tatataacta tctaaaatta tgttataaaa ctcttgtcta tttactgcat ccaaaccaat
     5221 tgttggctca tcaaatatag tgatagctga attagaacaa atacctatta tatttgaaag
     5281 tatggttttc atacctcttg acagttgttt atactttttt ttagtattta agtcaaaata
     5341 tttgcataat tcttcttcta agcctttatc ataattttta taaaaactag aagaaaattc
     5401 aaatacctga cctactttaa aatctggact ataaaattct ttttccctga caatacaaac
     5461 ttcttccaaa acttttatat cttcttttgg gttctttcct aatatttcta tctcaccttc
     5521 attttgaatt aactgattta ccattatatt taataatgtg gtttttccag ctccattctt
     5581 acctaataaa ccatatatct tatttttttc aaaatttaga gacatattat taaaaacatt
     5641 ttgtttctta aaactgtgat ataaattttc tatcttaata gaactactca tatattaatc
     5701 ctccttgaaa ttaattataa tattaactaa ttcttctcta tttatttcta attttttagc
     5761 ttcttgaagt agatttttta taaaattatg tttaaaattt tcttttctgg cttcttttat
     5821 aatagttttt gccccatctg atacaaacat ccctattccc ctcttcttat ataaaatatt
     5881 cttatcaacc aatatattta taccttttaa tactgtagct ggattaattt tataaagctt
     5941 tgaaagctct gttgttgatg gaacctgttc ttcttctttt attccatttg atagtatctc
     6001 atcttcaatt gctcgtgcta tctgaatgaa tataggttct tcattgttta atactaattt
     6061 cataggcttc tcctttctaa gttagttaat tactcaacta actaactgta acactatctt
     6121 atagtataat attacatctg tcaatacgaa ataaaaaaat atatgcaaac ttttatcaaa
     6181 aatacgtttt tatagatact aaaaaatatt atatttaaaa tactatatat gtatagcatg
     6241 aataaaaaaa tccaatagga aaacctattg gattttttat tcattaatat attaataata
     6301 tttatcaaat ttatacttaa ctcatctaat attttagaat acgctacata cttgtttata
     6361 tatatttttt aaattatact gtaataaata gatatgaaat tagtattata ttacactata
     6421 tatttcgtat ctataatact ctatttaaaa attcactttt tttattatat ctatttataa
     6481 tcttttaagt tactacttgt tatacagcta ctagtatata taaaatattt aatattccat
     6541 tggtttttcg ccatttaata ttttacatat tgaacttgta cctaatcttt gtgcacctgc
     6601 ttctatgaat ttttctgcat cagatattga ttttatcccc ccagcagcct ttatcttaac
     6661 accttcacca atgtgctttt tcataagttt tatatcttct agagttgctc catttgttcc
     6721 cataccagtt gatgtcttta taaagtctgc acctgaattt gttacgattt cacacatctt
     6781 tattttttca tattcattca gataacacgt ctctataatt acttttaata ccttatctcc
     6841 aatgacactt tttatttcct ttatttcatt ttctattttt tcatattcct cattttttat
     6901 atcagaaatg tttacaacca tatcaatttc aactgctcca ttttcaatag catcttttgc
     6961 ctcaaatacc tttactttag tagtttgata ccctaaagga aaacctatta ctgtgcaaat
     7021 atttactctt ccatttaaat actcagtagc actttttaca tatgatggtg gtatacaaac
     7081 tgatgctaca ttcattctta cagcttcatc acacaaagtt ttaatgtctt tcaatgtagt
     7141 tgttgccttt aacatagtat ggtctactgt ctgtaatata tgtttcataa aaacctccta
     7201 agtgtataat taagttactt ttttaaatgc aatttccttt tggcaatgtt taatatcatg
     7261 tcaacctctt caactttaaa aagaatcatc agtaatgtat aaactgagaa tccaattact
     7321 actgaaatcg tcagagatat taattcatta agtgtcccta aacccaatat accaaaaata
     7381 aatttataaa taaaatatgc tatgaaactc ataacacatg aagatactaa tgatttaaaa
     7441 attgctctta ttattttatc ttgcccaaaa tatcctactt ttctctttaa gtttaaaaaa
     7501 agtaacaata tacatgctat tgatgatgag ctagttgcca acgcaagacc tccatgggcc
     7561 ataggtttta tcaaagctaa atccaataat atattaactc caactgatac aataccattt
     7621 accataggtg tttttgtatc ctgtaaagaa taaaaaactt ttcctaatat gtctcttaat
     7681 ccaaaagctg tcatcccaac tgcataaaaa actaaagcag tagctgtcat ttgagtagct
     7741 ctagaatcaa atgctcctct ttcaaatata attctaacaa caggcttagc aaatacaata
     7801 gatgcaacag aaattggaat catagaaatt attatcatat ttacactaga cttaaccgaa
     7861 attgtaaact ttttttggtt attttctgca cttaatttcg ataacgtagg atacattaca
     7921 gatactattg aagctgtaaa tgttcctgta acaaacccat ttaacctatc tgcataagta
     7981 agtgctggaa tactcccctt aatcagtgta gatgctaatg ttgtatctac cagcgaattt
     8041 acttgattta cagctacgcc tataaataca ggtgataata gagccagtag ctttattaaa
     8101 ctttcatctt taaagtccaa ataaaataaa tatttatatt ttgttttatt cacaaaaaac
     8161 agataaaaaa atagctgaac taccatagct actacagccc caacaggaag tacatatggt
     8221 cctaacacag tacttaatac tattgaaata attattacaa tattataagg tatactacca
     8281 aacccaacta caataaaatt ttcttttatt tgtaaaaaag cagacataac actggtaatc
     8341 cctataaata ttatcccaat tataagtatt ctagtgaatt ttacagtaag taaaaatctt
     8401 tctccctcaa atcctatggc aaatatactt acaagttgtt ttgaaaaaat aactcctaat
     8461 attgctacaa ttatgcatat accaactatt atatttaaaa cattgtttaa aaactttaat
     8521 gcctcttttt ctcctttttt actatttata tcttgataca taggaataaa ggtagtaact
     8581 atagcagttc ctattgctgc aaatataata tttggtatat tcatagctgt taaataactt
     8641 tctgtataca agccagtccc ataaatcgaa gataacacta gttctctacc cattccaaga
     8701 attttagata ttatagtgac tatcattaaa taaaaagctg ctctagctac tctgctcatt
     8761 ttatcactcc actcttctcg cgtttcttca taattttttt agaagaaaag tacttatatc
     8821 agctatattt ctaaatacca ttttttattt tttatcaatc aataatttta aaagtagtgg
     8881 acacagaaaa tcactactta ttttcaaagt atattctatc atatattgtt cattatgaaa
     8941 actttttatc ttttgtctta tttctttagg tattttaaat atataaagac tagaaatttt
     9001 actccatgtg aaacttataa gcaataacaa tgtttacatg gagtaaataa aaactaccca
     9061 aaatttggat aaacaacttc ttctaatggt ttttcaatag gttttgcatc tatataattg
     9121 agaaatgcag tcttattact agaatttttt tgttccttat attcaatttt acctgttaat
     9181 agaatccatt ctccctcttt ccatttagga ggttgattca tattgacaat tggaccactc
     9241 gaaatcatgt cagctgcaca gcataccata attgaacgtg aagctataaa ctgatttttt
     9301 tcaagagaat catcataatg aacaaatact acatatgtta cttcttttcc cttaagaact
     9361 gactgttttg tatatagtaa attgagaaaa tcatatattt ctgtatcaaa tacgtcaaat
     9421 ttttcatcat atatccgttc ttctaatgtt ctatctagaa attcttcttt ttcatatatt
     9481 ttatcttgat atttttcttt tcccttatac ttttcaactt tcgttttttc tttatctata
     9541 ttcaaaccac tatacgatat tagtaatgtt gttattaaaa atgataaaat aatatattta
     9601 gtacttccat actgataaat atgcttaggt tttgttagta tcatgatgta agaaagtgct
     9661 atgaatagta atatacaaat tgaaattact aaatatggaa tcatccttgg gtgtatataa
     9721 tacacaattt cttttttcca taataaatat ataatctgaa ttaatactac cataactgtt
     9781 aataatttta tcaaacactc tttatttata tttttcataa tcaatcttac cttttctgaa
     9841 ccctcccttg actaaagtcg caaggttcct gcttcataaa attttacata atcaaacttt
     9901 aagataactg acgtaaataa taaaatttat tctttactat aaaaagataa atgctaaatt
     9961 ataattttat ttccatattg aaaaaaacaa aatacataat gtagcaaaca agtagaatta
    10021 taataaaaat ggtcataaat tttttgctga atcttgatga cattacaatt aaattcttaa
    10081 tatccaacaa aggaccaaat accataaatg gtattacgct cataagtggt agagaattag
    10141 taaatccctt agcaataaaa gcattacttg aagcacatat agaaagaaaa acagatgcaa
    10201 agaacatata caaaaatgca tagtcattag tgaaataacc tgaaaatgct ggattcattt
    10261 taagaatttg tgcaatagta gaaataaatg cgccaataat tataaacatc attattctta
    10321 aaaattcatt tcctatatga ataccaagtg caaggaatct aaacttctta ccttttttag
    10381 taatttttgt aatttcatat tcaatattat taaataaagg tattttattt tttttatatg
    10441 gagaaaagtt tttatttttt tcaacaaaat aaaaacttat tcctacaaga gcagaaagca
    10501 taatactaac taatattctt atccatatta ccttattatt ttcaggaaaa gcataaattg
    10561 ttgccaatat aacaatagga ttaatacttg atgatataca gaaaaaagtc atagcatgtg
    10621 atacttttaa acctttctgt ataaactttt ctgtaattgg aaccattgca caatcacata
    10681 atggtaaaat acaacccata aataaaaaaa ttggataaga taatacttta tgtctactag
    10741 caaaatatat aaacttctct tccgaaacaa gaaattgtat acaagaagaa aggacaatcc
    10801 caattaaaag aaatggtata gcctgaagta ttatgccaaa gaacattcta ttaaaatcat
    10861 tgatatcttg tactttaata atttgtattt tgacccccaa taataataaa aaaaacagta
    10921 taactataac aaacttaata attctataaa taagattttt aagttttaaa cttagaggaa
    10981 aaattttttt gtaattacca gattcatcaa tataaaaaat atccgcagaa ggttgtagtg
    11041 ctcttatata cgatgtaatt ttttctgttt tttcaattga attacttcca ctaatataaa
    11101 attcatcact atttattatc tgctcaacca tggaatctat atttctaaaa tacacatcaa
    11161 atgtatctgc attgactaaa cagtatatct tccctaaata acatgaatat ggaagcttta
    11221 tatcaagaaa ctcctctata ttccatgtcc cattgtattc aatcaaaatg taattataca
    11281 gattactaca tttcttaaga tattcttttg tcaaacaatt aatatcttta aatcgctcta
    11341 tctttatata gtttttcgaa gaaaatggaa ttgaatattc agtaaaacct tcctctaata
    11401 caattaataa aatattatct ttatcatcat attgtttatt ccttaaatat tcattaataa
    11461 acttagtctt tccagattct aagaatccag ctactatatc aattctagta ctcatttaaa
    11521 caaaagctct ttctaacttt tctttatttg gtttaaagcc aactacacaa atacaaccac
    11581 tacttatgta attacaaggt ataaccttta tttcgtcagg cacaaagcta aactcgtaaa
    11641 acaaaccatc ttcacattta aatatgccct ttgctctaat aatatttcca aaactaccac
    11701 tattgaactg ttctaaaatc tcttttatat attcatactt atagcttttt tctagtttaa
    11761 taccccaact actaaataca tcatctgcat tatgtactac atgattatgg tgacagttac
    11821 aatgcatatc attctcacta tgtactacag attttgtact cataatataa ttaacaatag
    11881 tctcatctaa taaattctgc tctaaagata caatatattt tttatgatac ttgttttgta
    11941 tggaacttat aacttccctt acttttattt catcacatac atcaactcta gataacaaaa
    12001 cagcatcaca atatttaatc tggtcatcat aaaactcttt aaagtttttt aaataaacac
    12061 tatatttcat tacatcaact attgtaaact tgtgatttat actcactaaa ttttgtttaa
    12121 ctaatggaat acaattttgt attacttcac tcagttttcc aactccagat ggttctacaa
    12181 ttactgtgtc tggcttaaat aattctataa cctcttttaa tgatttcgta aaatccgtat
    12241 ttaaagaaca gcaaatacaa ccagaataga gttctcttac ttgaatattt tcatttttaa
    12301 acagtagtcc atcaactcca acctctccat attcattttc aataagtact acttttttat
    12361 ctttataata agcacttagt aactttttaa taaaagttgt cttaccagag cctagaaatc
    12421 ctgatataat atcaattttt atcatttgca cctctccctt ttatttttta tttgttctta
    12481 ccttagtcta acgtttttat ttagactctc tttttgtaca gaagaataat cattaatgtt
    12541 atgctaccta aaataacaat agctcctcct ggactaagtc ctatataata tgaaatagtc
    12601 aatcctgata atacgaacaa cattgataag aaaattgcaa taagtaatgt tgatttataa
    12661 ctttttccta gtattaatcc acatgccact gggagtacta ataatgaaga aataatcaaa
    12721 gctccaacaa ctcttgcaga aattgaaata gtaagcccaa ttaaaattgt gaataatata
    12781 tctatttttc tagtatttac ataatgtttt tgtgcttctt tttcattgaa agcataaaac
    12841 ataaactcat aataatactt aaaaaatata atcaaaatca atatggtcaa ggcaattact
    12901 aaagcaaact ctctcccact aattgatata atactcccaa ataaaaaact atttatattt
    12961 acactagatt ttatgtagtt tgatagtatt ccactaattc ctacacctaa tgataaaata
    13021 acagcaatag ctatttcttc attatgtttc attcttttac ctataataga aactgaaaaa
    13081 gaggctatta tacaactcat aattgctcct attgtaggac taatgccaac cacaagacca
    13141 aagcatacac ctgcaagtgc tgtatgtgac aatgcatctc ccaacattga aaattttctt
    13201 aataccaaaa ttaaacctac acagggaatt gcaatgctca cacatagacc aacaaataag
    13261 gctctttgca taaatccgta actaagcata gtttaatacc tcgcatttta ttgtatgatt
    13321 ctcacatatt aatatcttgt ctgcaatttt ttttaattgt tcataatcat gtaatataag
    13381 aagaattgta tgtccattat tatgtaaatc cattatcata tccattacag atattttatt
    13441 tacctcatct agtgcattta aaggttcatc aaataatatt atatcgggat gattcataat
    13501 tgattttaat atcattgatt tttgtaattg accaccagat agagatttaa gctgtttttt
    13561 tgcataatgt tccatattat actttcttag tagcatcatt acatttttct tatttttaaa
    13621 caactttgac ttttctttat gttgatacat tagtaaaaac tccatcaatg ttaatggcga
    13681 accttctgcc atcactctat cattttgacc taaaaaactc gtagttaatg aatcaaaagt
    13741 aaaaatctct ccactttgta acttaacttc ctttgtaata attttaaata gtgttgattt
    13801 cccactacca ttttctccat aaactccaac caactcattt ttatttatat ttacatttac
    13861 acttttaaaa acattgcaat ctgtatacga aaatgctcca tcttttattt ccactatatt
    13921 tttcatactt attttgataa tgcatatacc aagttttgaa gattagtttt cataactgat
    13981 atatagtcct ccccctcttt tattttttta tcagataatt cttctaatgt gtttaatact
    14041 tttgtttctg aacctgtctc ttttgctatt gtctcagcta cttttggatt tacaagttct
    14101 tcataaaata taaccttgat tttattcttt ttagcagatt taataatttc tgccatacgt
    14161 gcagggcttg gttctgaatc tggagacaat ccttcaattg caatctgttt taaattatag
    14221 tctttgcaaa gataaccaaa tgcttgatga gccacaacta tagaacgtcc tttatatgga
    14281 agtagttcct ttttgtattg tgagtctaaa gcatcaaatt cacttgcata cttataataa
    14341 ttcttctcat aatatgtagc attatctggg tcttttttac aaaaagcatt ttttattttc
    14401 tctaactctt tctttgcatt acgaatacta agccatgtat gagggtccat actgtctgaa
    14461 cttttttctc ctttcatcaa atcaattcca tcacttgctt tgacaatcaa aatatttcta
    14521 ttatccaagc tttgtataac atcattgacc caatgttcca tattgtctcc attgtagata
    14581 aacatgtcag cattgctcaa tccttttata tcattcgttg ttggctcaaa atcatgaggc
    14641 tcaattccag caggtgtcat gtttttaaca tcaaccttag ttccaccaat ttttttagca
    14701 aaatcataca ttggataaaa actagtatat acactcaatt ttttgtttga gcttgtcttt
    14761 ttaacatctt tgaaactaca cccagtcaat agtaataaac tacagcaaaa taataataca
    14821 aatcttttca taatcgccct cctttttgca aatgctttgc atttgcattt ttataaggat
    14881 tatctcattt ttttctctat ttgtcaacta attgtgttgt taaattatat cttatgatat
    14941 atgataataa tagaatcata aaagaatggt ggttttatat atgaataatt atttgaaaaa
    15001 acacaactta aagagtacag aaaaaagaga ggctgtaatt aaagtattag aaaatagcaa
    15061 tgtacctatg acaatagaaa gtattcacga aaaagcaaat actatagtag aaatgaattt
    15121 aagtactatt tacagaacaa taacaacttt atgcgaaaag aatattacag ttttagtagc
    15181 tatacatgat ggaaaatctt attatcagct taacaagcat agacatgagc atgtattaat
    15241 ttgtaaagaa tgtaaagata ttattatcat tgacgaatgc cccttacatg agctagaaca
    15301 taaacttgaa gatgaaactg gttttgatat tatcgaacat aagttagaat ttattggcat
    15361 atgtcctaaa tgtaaaagaa cgaactctag aacataagct catctctctt ttttatttaa
    15421 cttgtttttc ttattaaata tataattata gattatataa agttaataag gaaagataat
    15481 tgcaaggctt aatgaaatta tttttagaaa aagtttctac taagtcttga aatacttata
    15541 tatattattt caataaaatt ttatttctaa gataataatt aaatctctat ttattaactt
    15601 aaatttaaat ttatttcttc aaaataaaca cttaaataag ctataaggaa taaccctatc
    15661 tgtaatttta gctactagcg aatgataaaa tatatcaatt ctgtagatac ttttaatgtg
    15721 tattgataaa gacttgtttc tgttaaacat aaaaagtgtg ctccttcctt tatatgatga
    15781 tatttgatat tttaattatc accacttttg gaagaataca cactttattt taaatacctt
    15841 ttgtactcta ttttatataa tttctaacaa cttaataaaa atgctttaat tgttttaact
    15901 tacttggatg tcttaacttt cttattgctt tagcttctat ttgtcttatt cgttctctag
    15961 taactccaaa tacccttcca atctgttcta atgtcttagg gtcatcatca tcaattccaa
    16021 atctcattct taaaacttgc tgttcttgct ctccaagacc tgtaagcaat tcttctattt
    16081 tttcttttaa attatgttgt tcaacctcat gcatcacaac atccttaaaa tcagcatctg
    16141 atggtataaa ttcaactaaa ctactatcct catcttcttt taatggagta tccaaagata
    16201 caacattttt atctcgtctt aataattcca atactttatc aacttctaat ccacatgcat
    16261 ctgcaatctc atctatagtt ggctctctta ataatacatt caaaagttct tgtttctttt
    16321 tctttacaaa atttattctc tgatgaagat gtataggtat cctaatagta ttttcgcaat
    16381 cgtcgatata tcttgttata ctttgtttta tccaccaagt tgcataagtg ctaaatttgt
    16441 atcctttttt ataatcgtac ttttctacag cttttataag tcctatattt cctgcttgaa
    16501 ttaaatccaa catatcaata ctatctcttt tatatctctt tgctatgctc actaccaacc
    16561 tataatttga atttataaat ttttcttttg ctactgaaga tgagttgatt atttccttag
    16621 ccagctctac ctcttctcct gctgacaaca ttttgtattc ctctatttcc tttaaataca
    16681 ttttcatcgc atttgataca tttgatgaac ccacacaaat ataattttca taattatttt
    16741 cttttttagt atccataatc aaaccccttc tattcataga atttgctaaa acaaaaataa
    16801 ataatttaaa cctacataag ctacaattac ataatatccc attcttcaaa tgaattgttt
    16861 tgtatattat gtatacaaat ttataaaaac catacctata atattattct acttctaaca
//
LOCUS       DAFNNB010000079        16690 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0080, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000079 DAFNNB010000000
VERSION     DAFNNB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16690
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0080"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            76..1029
                     /locus_tag="L3M60_002178"
     CDS             76..1029
                     /locus_tag="L3M60_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBN6226839.1"
                     /translation="MKKRYLVIGIIILSYFSLFIGAEEINIMHIFAKNQHKLMIFIMS
                     RVPRLISILIAGVGMSIAGLIMQQISKNKFVSPTTGATIDAAQFGIVICMLLVPTASI
                     FTKTIIAFVFSLVGTFMFMKIIGKLQFKNIIFVPLVGIMFGNIIGSMTDFIAYKYDLS
                     QNVSSWMQGDFSMILKGNYEILYITIPLIILAYIYANKFTVVGMGMDFATNLGLSYKR
                     IVNIGLIIVALVTVCVVVTAGNIPFIGLIVPNIVSLYMGDNIRASIWYTGLLGAIFVL
                     ICDIFGRIIIYPYEISIGLTVGVIGSILFLYLILRRNVNEA"
     gene            1019..1975
                     /locus_tag="L3M60_002179"
     CDS             1019..1975
                     /locus_tag="L3M60_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HBN6226840.1"
                     /translation="MKLSLNQKIYFVSILLFISIALFVIYGIDINHLEYALSQRIPKI
                     FAMILGGGCIAFTTVVFQTITNNQILTPSVLGLDSLYVMIQTIIVFIFGSSSSLIINE
                     NYNFIINVAIMIGASLLLYKMLFEKNKNNIFFLILVGMIFGTLFKSATTFIQVMIDPN
                     EFLALQTSIMASLNNINTNVLLIAFIIIIAIIPFIYDEIKYLDVLSLGKEQAINLGVD
                     FDKVVKKMIILIAILVSISTALIGPMTFLGLLLANIAREIFKTYKHTYLILGSMLIGM
                     ITLITGQFFIQHVFKFDTTLSVVINFIGGIYFIQLLLKGANR"
     gene            1972..2727
                     /locus_tag="L3M60_002180"
     CDS             1972..2727
                     /locus_tag="L3M60_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6226841.1"
                     /translation="MIEIKNIFKKYKNKNVVDDVSFNIEKGKITSFIGPNGAGKSTVL
                     SIVTRLIGGDGGEVIIEGKSLTNYSNKELAKKIAILKQSNNITLKLTIRELVGFGRFP
                     YSEGNLTKEDENYIDEAIEYMKLTDIQHKYLDELSGGQRQRAYIAMVIAQDTEYILLD
                     EPLNNLDMNHSVQMMKVLRGLCDELDKTIVLVMHDINFASCYSDNIVALKNGKVEKVG
                     RTDEIVNEKVLEDIYEMDFKIKNINGNRICIYF"
     gene            2819..3784
                     /locus_tag="L3M60_002181"
     CDS             2819..3784
                     /locus_tag="L3M60_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6226842.1"
                     /translation="MNKKAAIVAAVAIIGLVTVFALGGSKNNESKTSENSNNTIKIIH
                     NLGETDVKLNPKKVVVFDYSALDTMDTLGVAENLVGLPKASLPASLEKYKDEKYADLG
                     GLKEPDLEGIKSTNPDLIIINGRQEDFYEQLSKIAPTISTSKDDKEYLESVKNNIDKI
                     AKIFGVEEKANQEFSKIEKKIETLNKKVTDKNLNALTIMVNEGNLSVFGEESRFSILY
                     NSFGFKNKDKNIKESSHGQNITFEYIAKQNPEVMFVIDRGIATGSDIKESSTAKSVLN
                     NDIIKSMDAYKNDNIIYLDSPTWYVNDGGLTSLNKMIDDASKAVN"
     gene            4238..6097
                     /locus_tag="L3M60_002182"
     CDS             4238..6097
                     /locus_tag="L3M60_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6226843.1"
                     /translation="MKRFLRRIILVLFILLIFISFISIKLIHNVGDYGKLINYVGIVR
                     GASQRLTKLEMNHKPNDELIEYIDEILQELITGHGNYGLVLTDCNKYNEDLLLLEKKW
                     EDLNSEIKKVRMKEPNNQLLSISEEFFSLANDTVFEIENFSKEKSNYLMTLIIIISII
                     GILACIILILQYSKKMIKLEKLNVDLKNIAYKDELTGVNTIEKFKLDANQNICMHKDK
                     KFAVFYIDFENFKYINDIFGYDYGDMILKRYANLMMNDIGKYEIFAREIADRFVALRC
                     YIDKEDLVVRQRIVDSELINTTNEIKNKHSITVVSGICCIEDVNEKLSIDGLINRANF
                     AQKTVKNKPGTNYAFYNDSIRKKMIEENTIKSRIHEAIEKREFIVYLQPKVNLHNQKI
                     NCAEALVRWLTPDKGIISPAIFIPVLEKNFFIALVDKYVFEEVCKWIRKRLDENKPFV
                     QISVNVSRIQFYNTKFVETYSNIQNKYRIPKNTIEIEFTESVAFENQNHLLEIIHDLH
                     ENGFTCSLDDFGKGYSSLSVLKDLPFDALKLDSMFFKASLDKDKEKIVIKNIVHMLKE
                     LNITTVAEGIEYEEQVEFLRDIGCDLVQGFVFYKPMPILEFEEILDKEFVYNS"
     gene            complement(6297..6896)
                     /locus_tag="L3M60_002183"
     CDS             complement(6297..6896)
                     /locus_tag="L3M60_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HBN6226844.1"
                     /translation="MAIELKKGQKINLTKKENSDLGEILVNLNWNQKVQKKGFFGSLR
                     SSNIDLDLGCLFEMKNGVKGAVQALGNAFGSLDNPPFAQLDGDDRTGSNAQGENLRIN
                     GNKIKEIKRILIYAFIYEGVANWSEADGIVTIKQKQDSDLVVKLDEHKNGYNMCSIAL
                     IENVNDETFSVEKVVKYFKGHREMDTEFHWGLKWVAGRK"
     gene            complement(6999..7796)
                     /locus_tag="L3M60_002184"
     CDS             complement(6999..7796)
                     /locus_tag="L3M60_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBN6226845.1"
                     /translation="MKFKKLLCLLLCLVLTLAVVGCSKAKDDKKIVVGATLVPGGELL
                     EELKPLIKEKGYTLEVKNFDDYILPNEALNNGEIDANLFQHEPYLKEAVKAKGYKIMA
                     GKKLYVCPAILYSYKIKSVDEFKKGDTIAISNNPSSCSKNLRYLESIGLLTLPKGDGL
                     VSPKDIIENPKGIQFKELDIAQIPSSLPDVTAAFIDTTYAVPAGLDAKKNGIYTAPIN
                     DEYANLLAFRTEDKDSEKIKVLQDVLTSDKARSLIEEKYKGIVIPTF"
     regulatory      complement(7909..8014)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(8106..9092)
                     /locus_tag="L3M60_002185"
     CDS             complement(8106..9092)
                     /locus_tag="L3M60_002185"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HBN6226846.1"
                     /translation="MLLIYNEKTNPYFNLAMEEYLLKNFDEDLFILWRNEPSIIVGKN
                     QNTLSEINLEYIKEYSIPVVRRQSGGGAVFHDLGNINFTFIACNNNNFSDFRRFTQPI
                     IDLLKTLDVNAEFSGRNDLLIDGKKFSGNAQYNYKNKVMHHGTLLFSSEINDLSKALK
                     VKPIKFEGKGIKSVKSRVTNISEHLHSKMDILEFKDAIMNYLSKTNTDNTNYCLSELD
                     IEQIEKLTKSKYETWDWNFGNSPKYTLSNELKYSGGNVEFNLNVDKGIITSIKFFGDF
                     FGKCDVSFVENKLIGIRHEENALRNTLNSIEINDYFLGADTDILVSGILGVK"
     gene            9576..11306
                     /locus_tag="L3M60_002186"
     CDS             9576..11306
                     /locus_tag="L3M60_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HBN6226847.1"
                     /translation="MRNKKINIIFLTTIMFIMSTVMVFAEEDIDTIALANAEKFGILT
                     LIPPLVAIILAFITKNVIISLLIGILSGSFIIKASGINVFATFIQAFLDLVDRALVSL
                     ADPWNAGIILQVLAIGGVINLVAKMGGAKAIAEALAKRAKSAKGTQLITWFLGLLVFF
                     DDYANSLIVGPMMRPVADKMKISREKLAFIIDATAAPVAGLAIISTWIGLEVGLIHDA
                     FESISIDVDAFGIFLNTIPFRFYNILILAFIIISALLLKEFGPMRKAEIKSRSRKIDI
                     DLDEGVEELDDLAPKNGVKLSVWNAIIPIGTLIIVALASFYYSGYTSIMGGEDKALIQ
                     LFTNSPYSFEAIKEAFSASDASRALFQSALVASLVAIIMAVIKKIFTISEAIDVWIDG
                     MKSLVITGVILILAWSLSSVIKELGTAKFLIHLLSGSLPPFLLPSLIFGLGAIISFAT
                     GTAYGTMGILMPLAIPLAYSLNPDMSYVIVSTSAVLTGAIFGDHCSPISDTTILSSMG
                     AGCNHIDHVNTQMPYAIFTAVITIVFGYIPAGLGLPIYIVLPVAIAAIFVGIQIIGKR
                     VDEAEIELVE"
     gene            11416..11496
                     /locus_tag="L3M60_002187"
     tRNA            11416..11496
                     /locus_tag="L3M60_002187"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11450..11452,aa:Leu,seq:tag)
     regulatory      12275..12379
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            12589..15063
                     /gene="gcvPA"
                     /locus_tag="L3M60_002188"
     CDS             12589..15063
                     /gene="gcvPA"
                     /locus_tag="L3M60_002188"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HBN6226848.1"
                     /translation="MNELKRVSLYNIHKELGAKLVEFAGWEMPLEYEGINKEHDMVRK
                     SAGIFDVSHMGEVQIKGVESEKFIQNLVTNDISTLNINDIIYTPMCYENGGVVDDLLI
                     YKFGEEDYLLVINAGNIDKDVAWIIKQSERYNVDIKNISSEVSQLAIQGPKAEEILQK
                     ITDIDLNSIKFYKSIPSVKVCGCPCLVSRTGYTGEDGFEIYCENKYAEIIWNEVLKVG
                     GEDICPAGLGCRDTLRFEAALPLYGHEINEHISPVEGGLSIFVKTNKESFIGKSILSK
                     EKENGAKRKLVGFEMQGKGMPRNGYDIRIGDKIVGFVTTGCASPTTGKILGMGIIDSE
                     YAKVGNEIGIAIRKKVVPAVIAKKPFYKKQYKKDNIILNKENKFSYIPATSEDKSKML
                     KVVGLNSVDELFSDIPEEVKLKRDLNLEIGKSELEVSKIVKRLSEENLSLEDLTCFLG
                     AGAYDHYIPSIIKHITSRSEFYTAYTPYQAEISQGTLQVVFEFQSMIAEITGMEIANA
                     SMYDGATSAIEACIMAMNQTRKNKIVVSKTTHPETLSVLRTYLQYKDCEIVEIDFCNE
                     YGTTDIEKLKASVDKDTACVLIQTPNFFGIIEEMEEIEKITHENKAMLIMSVDPISLG
                     VLKTPGEIGADIVVGEAQSLGNPLNFGGPYVGFLASKSKYTRKMPGRIVGQSLDTEGK
                     IAYVLTLQTREQHVRREKATSNICSNQALNALVASIYMATMGKEGFKEVGMQSMKKAH
                     YTYNKLVQTGKYKPIFKGKFFKEFAIQGNLNIETINDKLLEENILGGYNLEYNYPELK
                     NSTLLCVTEKRSKEEIDKLVGIMEGL"
     gene            15063..16520
                     /gene="gcvPB"
                     /locus_tag="L3M60_002189"
     CDS             15063..16520
                     /gene="gcvPB"
                     /locus_tag="L3M60_002189"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HBN6226849.1"
                     /translation="MKEYNSLLIDISKKGRKAYSLPKLDIDDIKIEDMIDQNMIRKSE
                     LNLPEVGELELVRHYTLLSNKNFGVDTGFYPLGSCTMKYNPKINEDMAALPNFTGMHP
                     YQSSDTAQGSLSLMYDLSRRLSEITGMDEVTLQPSAGSHGEFTGLMIIKAYHENRGDH
                     KRTKVIIPDSAHGTNPASAAMANFDVIQIASDKNGAVDINALKEVLNDEVAALMLTNP
                     STLGLFEKNIKEIATLVHEAGGLLYYDGANMNAIMGITRPGNMGFDVVHLNLHKTFST
                     PHGGGGPGAGPVGVKKELVSFLPIPVIEKREDKYILNYDREKSIGKIKNFYGNFGVLV
                     RAYTYILTMGRDGLKEASEMAVLNANYIKESIKDDYILPIDTLCKHEFVLGGLPRGEA
                     NIKTIDIAKRLLDYGYHPPTMYFPLIINEALMIEPTESESIETLDSFIDAMKSVAKEA
                     KEEPELLKTAPHNTLVKRVDDARAVKKPILTWSQR"
BASE COUNT         6418 a         1902 c         2727 g         5643 t
ORIGIN      
        1 attattgata aactattgaa gtgaaaatga taataatttt cattttaatt aataaaagat
       61 ttttaaggag ggagaatgaa aaaaaggtat ttagtcatag gaataataat tttatcttat
      121 ttttctttat ttataggagc tgaagaaata aatataatgc atatatttgc aaaaaatcaa
      181 cataagctta tgatatttat catgagtaga gtaccaagat taataagtat attgattgct
      241 ggtgtaggaa tgagcatagc aggtcttata atgcaacaga taagtaaaaa taaatttgta
      301 tcacctacaa caggagctac aatagacgca gctcaatttg ggatagtgat atgtatgctt
      361 ttagtaccaa ctgcctctat ttttacaaag acaataatag catttgtatt ttctcttgta
      421 gggactttta tgtttatgaa aataataggt aagttacaat ttaaaaatat aatctttgta
      481 ccattagtag gtataatgtt tggaaatata ataggttcta tgacagattt tatagcatat
      541 aaatacgatt tgagtcaaaa tgttagctct tggatgcagg gtgacttttc tatgatactt
      601 aaaggaaatt atgagatatt atatataaca atacccctta taatactagc atatatttat
      661 gcaaacaaat ttactgttgt tggtatgggt atggattttg caacaaactt aggtcttagt
      721 tacaaaagaa ttgtcaatat aggtctgata atagtagcac ttgttacagt atgtgtagtt
      781 gttacagctg gtaatatacc ttttataggt ctaatagttc caaatatcgt atccttatat
      841 atgggcgata atataagagc aagtatatgg tatacaggtt tacttggagc tatatttgta
      901 cttatatgtg atatttttgg aagaattatt atttatccat atgaaatatc tataggactt
      961 acagttggag ttataggaag tatactattc ctttatttaa tattaaggag gaatgtaaat
     1021 gaagcttagt ctcaatcaaa aaatttactt tgtaagtata ttactattca tatcaatagc
     1081 actttttgta atttatggaa tagatataaa tcatttagaa tatgcattat cacaaagaat
     1141 accaaaaata tttgctatga tattgggtgg aggatgtata gcatttacta ctgtagtgtt
     1201 tcaaacaatt acaaacaatc aaatattaac accaagtgta ctaggtttag attcattata
     1261 tgtaatgatt caaactataa tagtatttat atttggttca agtagtagtc tgattataaa
     1321 tgaaaattat aattttatta ttaatgtagc tattatgatt ggagcttcat tattattata
     1381 taagatgcta tttgaaaaaa ataaaaataa tattttcttt ttaatcctag taggaatgat
     1441 atttggaact ttatttaaaa gtgcaacaac atttattcaa gtaatgatag acccaaatga
     1501 atttttagct ttgcaaacta gcataatggc cagtttaaac aatataaaca ctaatgtatt
     1561 gttaatagca tttataataa ttattgccat aatacctttt atatatgatg aaataaagta
     1621 tttagatgta ttgtcattag gtaaagagca agcaattaat ttaggtgtag attttgataa
     1681 agtagttaaa aaaatgataa tattaatagc aattttagtc tcaatatcaa cagcacttat
     1741 tggacctatg acatttttag gacttttact tgccaatata gccagagaaa tttttaaaac
     1801 atataaacat acatacttga ttttgggctc tatgttgata ggaatgatta cattgataac
     1861 aggtcaattt tttatacaac atgtatttaa atttgataca actttaagtg tagtaataaa
     1921 ttttatagga ggaatttact ttattcaatt actattaaag ggggcaaata gatgatagag
     1981 ataaaaaata tatttaaaaa atataagaat aaaaatgttg tagatgatgt ctcttttaat
     2041 atagaaaaag gaaagataac atcttttata ggaccaaatg gagctgggaa aagcacagta
     2101 ctatcaatag ttacaaggct tataggaggt gatggtggag aagtaataat agaaggaaaa
     2161 agcttgacta attatagcaa taaagaattg gcaaaaaaaa tagctattct taagcaatct
     2221 aacaatataa ctttaaagct tacaataaga gaattagttg gatttggaag atttccatat
     2281 agtgaaggta atctaactaa agaggatgaa aattatattg atgaagcaat agaatatatg
     2341 aaattgactg atatacaaca caagtattta gatgaattaa gtggaggtca aagacaaaga
     2401 gcttatatag caatggtaat agctcaagat acagagtaca tattgcttga tgaaccactc
     2461 aacaatttgg atatgaacca ttcagtacag atgatgaaag tacttagagg tctttgtgat
     2521 gagttagata agactatagt attagtaatg catgatataa attttgcatc atgttattca
     2581 gataatatag ttgcattaaa aaatggaaaa gttgaaaaag taggtcgtac agatgagata
     2641 gtaaatgaaa aagttttaga agatatttat gagatggatt ttaaaattaa gaatataaat
     2701 ggaaatcgaa tttgtattta tttttaaaat attatatata aaaataaact tgctatatta
     2761 ttttttataa aatttttaaa gtaaaataaa gattaataga aaagaggaaa atattgatat
     2821 gaataaaaaa gcagcgatag tagcagcagt agctattata ggtttagtga cagtgtttgc
     2881 attaggtgga agtaaaaata atgagtcgaa aacatcagaa aattcaaata atactataaa
     2941 gataattcat aacttgggag aaacagatgt aaaattaaat cctaaaaagg tagtagtatt
     3001 tgattattcg gctctagata caatggatac attaggtgta gctgagaatt tagtaggact
     3061 tccaaaggcg agtttaccag catccttaga aaaatataag gatgagaagt atgcagattt
     3121 aggaggttta aaagaaccag atttagaagg tataaagtca acaaatccag atttaattat
     3181 aataaatgga agacaagaag atttttatga gcaattgtca aaaatagcac caacaataag
     3241 cacaagtaaa gatgataagg agtacttaga atctgttaaa aataatatag ataaaatagc
     3301 aaaaatattt ggagtagaag aaaaagcaaa tcaagaattt agtaaaattg aaaagaaaat
     3361 agaaactttg aataaaaaag taacagataa aaatttaaat gctttaacta taatggtgaa
     3421 tgaaggtaat ttaagtgtat ttggtgaaga atcaagattt agtatattat acaatagttt
     3481 tggatttaaa aataaagata aaaatataaa agaatcaagt catggacaaa atattacttt
     3541 tgagtatata gctaaacaaa atccagaagt tatgttcgtt atagatagag gaatagctac
     3601 tggtagtgat ataaaagaga gctcaactgc aaaatctgtt ttgaacaatg atataataaa
     3661 atctatggat gcatataaaa atgataatat aatatattta gattcaccaa catggtatgt
     3721 aaatgatggt ggtctaacat ctttaaataa aatgatagat gatgcttcaa aagcggtgaa
     3781 ttaataatct gatataatca aaatgtgttt tttatgaaat gattatcaaa taaactagga
     3841 gttcttaaga actcctagtt tattttttgc ataataaaga tatagtttaa aaaataatat
     3901 tatttacaat aagataaatt tattcaaaat ctaataaaat atgaagttat actagacaaa
     3961 aagaataatt cttaaatata attaaattat atggaatata taataatata attttaataa
     4021 gttgtttata atatataaat tatattaata gtaataattt atatattatg agaaattttt
     4081 gtatatatag agcactctaa agatgaacta tgtaaaacaa ggtttatata ttctaattgt
     4141 gtagaaaatt ttattggaaa tgaatgcata ttattagaaa aatagtttag agatatccaa
     4201 taaacataaa aaataatata ggggataagg gtgttttatg aagagatttt tgaggagaat
     4261 tatcttagtt ttatttattt tattgatttt tataagtttc atttctatta aattaattca
     4321 taatgttgga gattatggga aactgattaa ttatgttggt attgttcgtg gagctagcca
     4381 acgcctaact aaactagaga tgaatcataa accaaatgat gaattaatag aatatattga
     4441 tgaaatttta caggaactga ttacaggtca tggaaattat ggcttagtac tcactgattg
     4501 taataagtat aatgaggact tgcttttatt ggaaaaaaag tgggaagatt taaatagtga
     4561 aattaaaaaa gtaagaatga aagaaccaaa taaccagtta ttaagtataa gtgaagagtt
     4621 tttttctctt gcaaatgata cagtgtttga aatagaaaac ttttctaaag aaaaaagtaa
     4681 ttacttaatg actttaataa taattatatc tattataggc atattagcat gtatcatatt
     4741 gattttacag tatagtaaaa aaatgattaa attagaaaaa ttaaatgtag atttaaaaaa
     4801 tattgcatat aaagatgaac taacaggagt aaatactata gaaaaattta aattggatgc
     4861 aaatcaaaat atttgtatgc ataaggataa gaaatttgct gtgttctata ttgattttga
     4921 aaactttaaa tacattaatg atatatttgg atatgattat ggtgacatga tactgaaaag
     4981 atatgctaat cttatgatga atgatatagg aaaatatgaa atatttgcaa gagaaatagc
     5041 agatagattt gttgcattaa gatgctatat agataaggaa gatttagtag taagacaacg
     5101 aattgtagat agtgaactga ttaatacaac aaatgagatt aaaaataaac atagcattac
     5161 tgttgttagt ggaatttgct gtattgaaga tgtaaatgaa aaattaagta tagatggctt
     5221 aataaataga gcgaactttg cacaaaaaac tgttaagaat aaaccaggta ctaattatgc
     5281 tttttataat gatagtattc gtaaaaaaat gattgaagaa aatactatca agagtagaat
     5341 acatgaagct attgaaaaaa gagaatttat agtttattta cagcctaaag tcaatttaca
     5401 taatcaaaaa ataaattgtg cagaagcctt agttcgttgg ttgactccag acaaaggtat
     5461 tatatctcca gcaatattta ttccagtatt agaaaagaat ttttttattg ctttagtaga
     5521 taaatatgtt tttgaggaag tttgcaaatg gattagaaag agattagatg aaaataaacc
     5581 ttttgttcag atatcagtta atgtttccag aatacaattt tataatacta agttcgtaga
     5641 aacttattcc aatatacaaa ataaatacag gatacctaaa aatactatag aaattgaatt
     5701 tacagagagt gttgcttttg agaatcaaaa tcatcttttg gaaattatac atgacttgca
     5761 cgaaaatgga tttacttgtt ctttagatga ttttggaaaa ggttattcgt ctttaagtgt
     5821 tttaaaagat ttgccatttg atgctttaaa gcttgactct atgtttttca aggctagttt
     5881 agataaagat aaagaaaaaa tagtaataaa aaatattgta catatgttaa aggagcttaa
     5941 tatcacaact gttgctgagg gtattgaata tgaggaacaa gttgaatttc ttagggatat
     6001 tgggtgtgat ttagttcagg gatttgtatt ttataaacca atgccgatat tagagtttga
     6061 agaaatattg gataaagagt ttgtttataa tagctaaaat gtactttgta aaatacaaac
     6121 taattaagta aaattattct acactgaaca aattttcatt ttatttacca tataagaatg
     6181 cgtaatgata taagtatttg aaaagccacc attatatgaa aattaaaaag aaaataaata
     6241 atttttcgat aaaaaagaga ctatgagaaa agtcacccat agtctcctaa atatagttat
     6301 tttcttcctg caacccattt taagccccaa tgaaattcag tatccatttc tctatgacct
     6361 ttgaagtatt taactacttt ttcaacacta aatgtctcat catttacatt ttctatcaat
     6421 gctattgaac acatattata gccattttta tgttcatcaa gtttgacaac taagtctgaa
     6481 tcttgttttt gtttgattgt tacaattcca tcagcttcag accagttagc cacaccttca
     6541 tatataaaag cataaataag tattcttttt atctctttta ttttattacc atttattctt
     6601 aaattttctc cttgtgcatt acttcctgtt cggtcatctc catctaattg agcaaaaggt
     6661 ggattgtcta aactaccaaa agcatttcct aatgcttgaa cagcaccttt tacaccattt
     6721 ttcatctcaa ataggcaacc caaatctaaa tctatattac tgcttcttaa agagccaaaa
     6781 aaaccctttt tttgtacttt ttgattccaa tttaaattaa ctaagatttc acctaaatcg
     6841 ctattttcct tttttgtaag gtttattttt tgtccctttt ttaattctat agccatagta
     6901 atcttcctcc acaaacatag atttatttaa atggtagcac ttaataagtc aatattcaac
     6961 aagattagaa taataatatt aaaattatac taaatatact aaaaagtagg aattacaata
     7021 cctttgtatt tttcttcaat taagcttctt gctttatctg atgtaagaac atcttgtaga
     7081 actttaatct tttcactatc tttatcttca gttctaaatg ctaaaagatt tgcatactca
     7141 tcatttatag gagctgtata aattccgttt ttcttggcat caagaccagc aggtacagca
     7201 taagtagtat ctataaatgc agcagttaca tctggaagag aagatggaat ttgagcaata
     7261 tctaactctt tgaattgtat tccctttgga ttttctatta tgtccttagg gctaactaat
     7321 ccatcacctt taggcaatgt taaaagtcca atactttcaa ggtatctaag gttcttagaa
     7381 caagaagaag gattattgct tattgcaatt gtatcacctt ttttaaattc atcaacagat
     7441 tttatcttat aagagtaaag tattgctggg catacatata gttttttacc agccattatt
     7501 ttgtaacctt tggccttaac agcttctttt agataaggtt catgttgaaa tagatttgca
     7561 tcaatctcac cattatttaa agcttcgttt ggaagtatgt aatcatcaaa gtttttaacc
     7621 tctaaagtat atcctttttc cttaataagt ggttttaatt cttctaataa ctctccacct
     7681 ggaactaaag tagcaccaac aacaattttt ttatcatctt tagctttaga acatccaacc
     7741 acagctaaag ttaatactag acataataat aaacataata attttttgaa tttcatataa
     7801 attccctcct gaatataatt gattagttaa aaataaaata ctattcttta tcagactgca
     7861 aaataaaaat aagcctgagt ttaaactcag gctttaaaag ctaatttaga ctcatctttc
     7921 agtataatac tgcaggattt agcaccaatg tatataaaaa tatactggtt gctgggtttc
     7981 atagggccag tcccttcacc actctggata agaagtatca atttttaatt atgttataaa
     8041 tattatcatg attttttacg tctgtaaata taattttata aatattttaa aatctttagt
     8101 gtattttatt taacacctaa aattccagaa actagaatat cagtatcagc acctaagaaa
     8161 tagtcgttta tttcaataga atttagggtg tttctcaaag cattttcttc atgtcttatg
     8221 ccaataagtt tattttctac aaatgaaaca tcacattttc caaaaaaatc tccaaaaaat
     8281 tttatgctag ttattatgcc tttatcaaca tttaaattaa actcaacatt tccaccagaa
     8341 tattttaatt catttgaaag agtatatttt ggtgaattac caaaattcca atcccaagtt
     8401 tcatattttg atttagtaag cttttctatt tgttctatat caagttcact caaacaataa
     8461 ttagtattat cagtgtttgt tttagaaaga tagttcatta tagcatcttt aaattctaat
     8521 atgtccattt tagagtgtaa atgttcactt atattggtta cacgagattt tacagatttt
     8581 attccttttc cttcaaattt tataggttta acttttaatg cttttgagag gtcatttatt
     8641 tcagatgaaa ataaaagtgt accatgatgc attactttgt ttttgtagtt gtactgagca
     8701 tttccagaaa atttcttgcc gtctataaga aggtcatttc ttccagaaaa ttcagcattg
     8761 acatctaatg ttttaagtaa gtctattatt ggttgagtga atcttctgaa atcactaaaa
     8821 ttattattgt tacaagctat aaatgtaaaa tttatatttc ctaaatcatg aaatacagcc
     8881 ccaccaccag actgtctcct aacgacaggt atagaatatt cttttatata ttctaaattt
     8941 atttcagaaa gtgtgttttg gttttttcca acaattatag aaggttcatt cctccataaa
     9001 atgaataggt cttcatcaaa attttttaga agatattctt ccattgctaa attaaaataa
     9061 gggtttgttt tttcattata aattaataac attaaagtag ttcctccata taattgataa
     9121 aatttttatt atgagtacaa ttaattttat aattcgacaa taatgtaaaa actcctttaa
     9181 attagaaatt ttaaaaacta ccttgcaaag tttaaataaa taatatatac tcaaaatatg
     9241 tttaataaaa gtgaatagaa gggtagaggc gcaataatct atcagtacta ataaggagtt
     9301 aagcacatag agttattagg aaaggagata ttgccgaagt tttataatcg atgctttaag
     9361 attatattgc tgggggtaca tagaaaatat gtacaactgt cacaggttta tttgtggtga
     9421 gctatcattt aaggaattat attatattta tttttactat tgttgatata ttgtaatgtt
     9481 taagccttga gtgtttacca ctcaaggctt ttttagtttt attgtaacat aataccctat
     9541 acagttcaca aaattaaaag gagagggaga tttatatgag gaacaaaaaa attaatatta
     9601 tttttctgac gacaatcatg ttcataatgt caacagtaat ggtttttgca gaagaggata
     9661 tagacactat tgcactagct aatgcagaaa aatttggaat tttaacttta ataccaccat
     9721 tagttgctat aatacttgca tttataacta aaaacgtaat aatttcttta cttataggta
     9781 ttctttctgg tagttttata attaaagcta gtggtattaa tgtttttgcg acatttatac
     9841 aagcattttt agatttagta gacagagctt tagtatcttt agcagaccct tggaatgcag
     9901 gaataatact acaggttttg gcaattggag gagtaataaa tcttgttgct aaaatgggag
     9961 gagctaaagc tatagctgaa gcacttgcta aaagagcaaa gtcagcaaaa gggacacagc
    10021 ttataacatg gtttttgggg cttttagtat tctttgatga ttatgccaat tcattaatag
    10081 taggtcctat gatgagacct gttgcagata agatgaaaat atcaagagaa aaactagcct
    10141 ttataataga tgctacagca gcaccagtag ctggtttagc tataatatct acatggatag
    10201 gtcttgaggt tggtttaata catgatgctt ttgaaagtat aagtatagat gtagatgcat
    10261 ttggtatatt tttaaataca ataccattta gattttacaa tatattaata ttagcattca
    10321 ttataatatc agccttatta ttaaaagaat ttggaccaat gagaaaagct gaaataaaat
    10381 ctagaagtag aaaaattgat atagaccttg atgaaggcgt agaagagttg gatgatttgg
    10441 ctccaaaaaa tggagtgaaa ttgagtgtat ggaatgctat aatcccaata ggaactttga
    10501 taatagttgc attagcatca ttttattata gtggatacac atctataatg ggaggagagg
    10561 ataaggctct gatacaacta tttacaaatt ctccatattc ttttgaagca ataaaagaag
    10621 catttagtgc atctgatgca tctagagcat tattccagtc tgcacttgtt gcaagtttag
    10681 ttgctattat aatggctgta ataaaaaaga tttttacaat atcagaagca attgatgttt
    10741 ggatagatgg aatgaaaagt ttagtaatca ctggtgtaat attaatactt gcatggtcat
    10801 taagttcagt aataaaagag ttaggtacag ctaaattttt aatacattta ttgtcaggtt
    10861 cactaccacc attcttatta ccaagtttaa tatttggttt aggagcaata atatcttttg
    10921 ctactggaac agcttatgga acaatgggta tacttatgcc acttgcaata ccattagcat
    10981 actcattaaa tcctgatatg tcttatgtaa tagtaagtac tagtgcagtt ttaacaggtg
    11041 caatatttgg agaccattgt tctccaattt cagatacaac aatactttct tcaatgggag
    11101 ctgggtgtaa tcatatagac catgtcaaca ctcaaatgcc atatgctata tttacagcag
    11161 taattactat tgtatttggg tatataccag ctggacttgg attgccaata tatatagtat
    11221 taccagttgc aatagctgct atatttgtag gtattcaaat aattggtaag agagtagacg
    11281 aagcggaaat agaacttgta gaataaagat ataaataagt ttcacctttt attatcatta
    11341 ttttgttagt ttttagataa ttttaaaaag ttattgaaaa taatagcgaa atatagtaaa
    11401 ataaaagagt gataagcgga tgtggcggaa ttggcagacg cactagactt aggatctagc
    11461 gccattggcg tgggggttcg actcccttca tccgcacttt acagtactta attgtgctga
    11521 aaaaaactgg ggttgctggc tttcggagtg ggctatcccg tttttatttt ttggtatata
    11581 tgaaagattg gcactttaat atgaagtgct ttttttatag gcaaaaaaca gtacatttat
    11641 ttctgtttta ttgtcattaa tttattagaa catgctttag ttaagaatat gttaataagc
    11701 aatgttcaca ttcttgataa aatatggaat atatgtatct ttactaaggc agaaatgaaa
    11761 taactaaact actaaatgat attataagat gataaataag tttcagttta ttggatggtt
    11821 gaagtaatca gaaattggaa ttttatagtc ttaatataat gtatgaaatt ttatagcaat
    11881 gtatttcata agtataaagt gactaaaatt ttaaaaaata tacaaattct atagaagcta
    11941 aataatttca actctatttt aaaaatctta tttttcacaa gtttaaaatt gtaaacgatt
    12001 tcatcaagtc tatgaatata cttttaacat gttatagtaa atctcaatat aaatttacat
    12061 ttgcttcctt gtatgttatt ttaataataa attaagtgaa aatgttattt tacattaata
    12121 tataatattt ttgcataaga atgaataata taaaagcgaa ataattgata ataaaaactt
    12181 aacaaaaaaa ttaatacata aaaaaaaata aatataatgt gttgacattt aattgaaata
    12241 ttcatacaat aaaattgaaa ataaatattt ccaaatgaga ttagcgagag agtcctttat
    12301 acgaaaaggc gccgaagaag aaatttaaga aatgccaatt tttagagaag ctttcaggca
    12361 aaaatatcgc taattgatgg gactctggag agacttaagt taagatacta taagtcaccg
    12421 aagaagcaaa ataatattaa attattaaat ctttcaggta tacaaacagg gatatatagg
    12481 ctaggattat tttccatgct gatatatctt ttttttatga aaaagtacag gaaataatat
    12541 caaagttact taaatacagt aggtctaatt ttatagggag ggttatatat gaatgaattg
    12601 aaaagagtat ctttgtataa catacacaaa gagcttggag caaaacttgt tgaatttgct
    12661 ggctgggaaa tgcctttaga atatgaaggt ataaataaag agcatgatat ggtcagaaaa
    12721 agtgcaggta tatttgatgt atctcatatg ggagaggtac agataaaggg agtagagtca
    12781 gaaaaattta tacaaaacct agttacaaat gatataagca cattaaatat aaatgacatt
    12841 atatatacac ctatgtgtta tgaaaatggt ggagtagttg atgatttatt aatatataaa
    12901 tttggtgaag aagattattt attggtaata aatgcaggca atatagataa agatgtagca
    12961 tggataataa aacaaagtga aagatacaat gtagatataa aaaatatatc tagtgaagtg
    13021 agtcaattag caattcaagg acctaaagca gaagaaatcc ttcaaaaaat cacagatata
    13081 gacttaaact caataaaatt ttataagtca attccatcag taaaagtatg tggttgtcct
    13141 tgtttagttt caagaacagg atatactggg gaagatgggt ttgagatata ctgtgaaaat
    13201 aaatatgcag aaataatttg gaatgaagtt ctaaaggttg gaggagaaga tatatgtcca
    13261 gctggactag gttgtaggga cactttgaga tttgaagcag ctctacctct atatggacat
    13321 gaaataaatg aacatatatc tccggttgaa ggtggtttat ctatctttgt gaaaacaaat
    13381 aaagaatcat ttattggcaa atcaatttta tcaaaagaaa aagaaaatgg agccaaaaga
    13441 aagttagtag gatttgaaat gcaaggtaaa ggaatgccta gaaatggata tgatataagg
    13501 attggtgata aaatagttgg atttgtaaca acaggatgtg catctccaac aacaggaaaa
    13561 attttaggta tgggaattat agattctgag tatgcaaaag taggaaatga aataggtata
    13621 gcaatcagga aaaaagttgt tcctgctgtg atagctaaga aaccttttta taaaaaacaa
    13681 tataaaaaag ataatattat attaaacaaa gaaaataaat tttcatatat acctgctaca
    13741 agtgaagata aatcaaaaat gttaaaagta gttggattaa attcagttga tgaattattt
    13801 tcagatatac cagaagaagt aaaattaaaa agagatttaa atttggaaat tggaaaatct
    13861 gaattagagg taagtaaaat agtaaaaaga ttgtcagaag aaaatctaag cttagaagac
    13921 ctgacttgtt ttcttggtgc tggtgcatat gaccattata taccatcaat tataaaacat
    13981 ataacgtcaa gatctgaatt ttatacagca tacactccat accaagctga aataagtcaa
    14041 ggaactttac aagtagtttt tgaatttcaa tctatgattg cagaaattac aggtatggaa
    14101 attgcaaatg catcaatgta tgatggagca acttctgcaa tagaagcatg tataatggca
    14161 atgaatcaga ctagaaaaaa taaaatagtg gtgtcaaaaa ctactcatcc tgaaactttg
    14221 tcagtattaa gaacttattt acaatataaa gattgtgaga tagtagaaat tgatttttgt
    14281 aatgaatatg ggactacaga tatagaaaaa ttaaaagctt ctgtagataa agacacagca
    14341 tgtgttctta tacagactcc taatttcttt ggaattattg aagaaatgga agaaatagaa
    14401 aagataactc atgaaaacaa ggctatgtta attatgagtg tagacccaat atcattagga
    14461 gttttaaaaa caccaggtga gataggtgca gatattgttg ttggagaagc ccaatcactt
    14521 ggaaatcctc ttaattttgg aggtccttat gtaggatttt tagctagcaa atctaaatat
    14581 acgagaaaaa tgccaggaag aatagttggg caaagtttag atacagaagg taagattgca
    14641 tatgtattaa ctttacaaac tagagaacag catgtaagaa gagaaaaagc aacttctaat
    14701 atatgttcaa atcaagcttt aaatgctctt gtggcttcaa tatatatggc tactatgggt
    14761 aaagaaggat ttaaagaggt tggtatgcag tctatgaaaa aagcacatta tacttataat
    14821 aagcttgtac aaactggaaa atataagcca atatttaaag gcaagttttt caaagaattt
    14881 gctatacaag gaaatctcaa tatagaaact ataaatgata aactcctaga agaaaatata
    14941 ttaggtggtt ataatttaga atacaattat ccagaattaa aaaattcaac tttactttgt
    15001 gtgactgaga aaagaagcaa agaggaaata gataagttag ttggaataat ggaggggtta
    15061 taatgaaaga atataatagt ttattgatag atatatctaa aaaaggtaga aaagcatact
    15121 cacttccaaa actagatata gatgatataa aaatagaaga tatgattgac caaaatatga
    15181 taagaaaaag tgaattgaat ttgccagaag tcggtgaatt agaattagtt agacattata
    15241 cattattatc aaataaaaac tttggtgttg atacaggttt ttatcctttg ggttcttgta
    15301 ctatgaaata taatcctaaa ataaatgaag atatggcagc acttccaaac tttactggaa
    15361 tgcatccata tcaatcttca gatacagctc aaggttctct ttccttgatg tatgatttat
    15421 caagaagact ttcagaaatt acaggtatgg atgaagttac actacaacct tcagcaggtt
    15481 cacatggtga gtttacaggg cttatgatta taaaagcata tcatgaaaat agaggtgacc
    15541 ataagagaac caaagtgata attccagatt ctgcacatgg aacaaaccca gcaagtgcag
    15601 ctatggcaaa ctttgatgtg attcaaatag catctgataa aaatggagct gtagacataa
    15661 atgcattaaa agaagtttta aatgatgaag tagcagctct tatgcttact aatccaagta
    15721 ctcttggttt gtttgaaaaa aatataaaag aaatagctac tcttgtacat gaagcaggag
    15781 ggcttttgta ctatgatggt gcaaacatga atgctattat gggtataaca agacctggaa
    15841 acatggggtt tgatgtggtt catcttaatc ttcataagac tttttctact cctcatggag
    15901 gtgggggacc aggagctggt cctgttggag ttaaaaaaga gttggtatcc tttttaccaa
    15961 taccagtaat tgaaaaaaga gaagataagt atatattaaa ttatgataga gaaaagtcta
    16021 ttggaaaaat taaaaacttt tatggtaatt ttggagtgct tgtaagagct tacacttata
    16081 tattaactat gggaagagat gggcttaaag aagctagtga aatggcagtt ttaaatgcta
    16141 attatataaa ggaaagtata aaagatgatt atattttgcc aatagatact ttatgtaagc
    16201 atgagtttgt attaggtgga ttaccaagag gagaggcaaa tataaaaact attgatattg
    16261 caaaaagact actagattat ggataccatc ctccaacaat gtactttcct cttattataa
    16321 atgaggcact tatgatagag cccacagaaa gtgagagtat agagacatta gatagcttta
    16381 tagacgctat gaaaagtgtt gctaaagaag caaaggaaga accagaacta ttgaaaactg
    16441 ctccacataa tactttagta aaaagggtag atgatgcaag ggcagtaaaa aaacctatac
    16501 tgacatggtc acaaagataa ataattaaaa aatagagatt taaagcataa ataaatttga
    16561 atttgcaaaa aaagctatct aacttaatct agtttaggta gtttttttgt attggtagat
    16621 ataagatttt ctattaaaaa tttaaattat attattactc atataaaata ttaatgtaat
    16681 gcttatgtac
//
LOCUS       DAFNNB010000080        16580 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0081, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000080 DAFNNB010000000
VERSION     DAFNNB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0081"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(861..1571)
                     /gene="rgaR"
                     /locus_tag="L3M60_002190"
     CDS             complement(861..1571)
                     /gene="rgaR"
                     /locus_tag="L3M60_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RgaR"
                     /protein_id="HBN6226850.1"
                     /translation="MFRVVICDDEKIQRSILKEFTEKLLSERCLNYQILEFSCGEDLI
                     SKYPEKIDIIFLDIQMKDINGIEVAKKIRKFDEKVEIIFTTAFSEYAPQGYEVRAYRY
                     LVKPIEYSNFAMGVNLCIDNLQRKKERYIVLNSKKGFNRILIDSILYVETVKRDLVVH
                     TIGRDYKANMSMKQAEKLLCENGFFRCHTSFIVNLRRIEDLRDNMITINEQFIPISKY
                     RLKDLKVALTSLLCDIMY"
     gene            complement(1995..4661)
                     /locus_tag="L3M60_002191"
     CDS             complement(1995..4661)
                     /locus_tag="L3M60_002191"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBN6226851.1"
                     /translation="MENTNLSKTYNPKEFEARLYKKWMDEGYFKSKPNPDKKPFTIMM
                     PPPNITGQLHMGHALDHTLQDILIRWKRMDGYEAFWLPGTDHASIATEVKVVERIKKQ
                     EGKTKYEIGREEFLKRAWEWKDEFGGKISNQLKQLGDSCDWDKERFTMDEGCNEAVIE
                     FFVSLYEKGHIYRGNRIINWCPDCKTTLSDAEVEHEEHDGNFYHIKYPLKDSEDFLEI
                     ATTRPETMIGDTGIAVNPEDDRYKHLIGKTAILPLVGRELPIVADSYVDLEFGTGAVK
                     MTPAHDPNDFEVGLRHNLEQLNTMNEDGTMNEVCGKYEGMDRFECRKAIVADLKEQGY
                     LIKIKEHNHNVGTCYRCHTVVEPRLSEQWFVKMEELAKPAIDILKKGELEFVPEKFDK
                     TYLQWLENIRDWCISRQLWWGHQIPAYYCQECGEIVVARKMPDKCPKCGSTHFKQDED
                     ALDTWFSSALWPFSTLGWPNKTEALDYYYPTSVLVTGYDIIFFWVVRMAFAGMFCMNE
                     KPFDHVLVHGLVRDSQGRKMSKSLGNGIDPLEIIEQYGADALRFTLTTGNSPGNDMRF
                     YMERVEFARNFANKLWNASRFVFMNIDEDIIKNMTRESVKEDLTLADKWIISRANNIV
                     KEATNNMDKFDLGIALQKIYDFTWSEYCDWYIEMVKPRLYGEDTNAKSAALYTLTYVL
                     EKILKLLHPYMPFITEEIYTHLPTVEGCIIVSEWPKYNEEDNMAEEEDMMNLLMEGIR
                     SIRNVRAEMNVPPSKKAKLIIIPSEEKIEAIELGKDYFITLASASNVEIAKDKSNVPE
                     DAVGVVIDGVEIFIPLNELVDFEKEIERLSKEKKKLEGEIKRVNGKLANQGFLAKAPE
                     SLIEEEKAKKEKFEEMIKSVEERLTNLESKIK"
     misc_binding    complement(4736..4998)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5300..6127)
                     /locus_tag="L3M60_002192"
     CDS             complement(5300..6127)
                     /locus_tag="L3M60_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6226852.1"
                     /translation="MKIKDNKTVFHVFVRIFLVAVLILTGTLLEEKINNVPVENISSK
                     GESTLELLSQSNTLGEIIYKASQEETKGPGATSEKVAYITIDDGPSKYTNALLDILKE
                     NDVKATFFMLNQNMKNHKEEVRRVLEEENSIGFHSVSHDIHKLYKSPEVTVGEFNTCN
                     NTLQEITGQSSKLIRLPYGSKPYMPEGSYDKLMANDYLIWDWNLDTQDWKSSTSQIVS
                     NILYYGRKRDELVVLIHEKEQSLNALNNIIRILKERGYTILPITENISPKNFWQENL"
     gene            complement(6175..7611)
                     /locus_tag="L3M60_002193"
     CDS             complement(6175..7611)
                     /locus_tag="L3M60_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron hydrogenase"
                     /protein_id="HBN6226853.1"
                     /translation="MNTKNDNSKILEIYKMKLFGELVKAVWNDDIEDIENLPNKILNE
                     DLKDDLNNYFSGKFDKDTMVNLIRVTMGLDVTGDTDKNLKDSLHEAVNLDEVKRPIIS
                     IISDACKYCDENNKEDESCKIRSKHINCNEEDVCSACGDCVSRCKLGAISDKIEFIPM
                     IKLLKDIKSPVYAIVAPAFIGQFGKDVSPGKLRTALKKIGFEDMIEVALAADMLTAKE
                     AYDYYEHMKKDEEGCFITSCCCPIWVSLIQNSFPQILENVSPSVSPMIACGRAVKILN
                     PDAKVVFIGPCMAKKKEAMLEDIKDAVDFVLTFKELQEIFDALELNPAIMDESNRVEA
                     SLSGRVYARTGGVSKAVKLSVRRIDKNIKFEEKAFEGTKECMDGLKKVLNKEIDATFI
                     EGMGCVGGCVGGPKRILSVEEGTKNVDEYSESTNMETPFDNLNVLQFLTSLGIKRVES
                     LGEKEEEKVKKIFSRDIKSNRKENKYKK"
     gene            complement(7698..8567)
                     /locus_tag="L3M60_002194"
     CDS             complement(7698..8567)
                     /locus_tag="L3M60_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBN6226854.1"
                     /translation="MKLQKMGMRTIKTGISVTLCMILGDYLVENMFYSLVACIISVQD
                     TVKGSLKTGINRVEGTILGGIIGFIFAIIKAGDPFLCGLGVMCSIYLCNLFKIKSVVV
                     ACVTFLAIHLGVGNSDPVYYSLHRVLDTSVGVLFGVLVNYYVARPNYLNSVIDEFKKI
                     EELSMLLIEDKIMRKENLNVEKFEEEVEKLEEIYSKLIDELDYSLSETNIEKIENALR
                     ICREIHFHMQSIELLENKLYLKEHTYKYLKKTYNVDKLDWNIDEEKSPVFNYHLMKIM
                     IEIELLRKLNKSY"
     gene            complement(8725..9378)
                     /gene="phoU"
                     /locus_tag="L3M60_002195"
     CDS             complement(8725..9378)
                     /gene="phoU"
                     /locus_tag="L3M60_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBN6226855.1"
                     /translation="MVNNSLDISIDTLKEQTVLMIEKCEEAIEKSVTCMIEKDLEGAR
                     KVVEDDEEIDKLREEIRDKSIELIVLKQPMARDLRFIYSLGNISIELERIGDYAANIA
                     REMLKIGGEAYIKELIDIPKMAKECKSMMSQTREALILFDASIAYDVGTKDDIVDGLY
                     EQVQIDCLKIMHENSNTIDQGVKLLLIGRYLERIGDHITNVCEKIIFAIDGKMVEIG"
     gene            complement(9406..10170)
                     /locus_tag="L3M60_002196"
     CDS             complement(9406..10170)
                     /locus_tag="L3M60_002196"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HBN6226856.1"
                     /translation="MELIDKIKMSVKDLDLFYGDKQALKKINMDIKENKVTALIGPSG
                     CGKSTFIRTLNRMNDLIEDVTIKGSISVDGEDIYTSDDVINLRTKVGMVFQKPNPFPM
                     SIYDNVAYGPRIHGLRDKKQLDKIVEESLKGAAIWDEVKDRLKSSALGLSGGQQQRIC
                     IARAIAMRPEVILMDEPTSALDPISTLKVEELIEDLKKDYTIVIVTHNMQQAARISDE
                     TAFFLNGEVIEFSDTKTIFTTPVDKRTEDYITGRFG"
     gene            complement(10189..11013)
                     /gene="pstA"
                     /locus_tag="L3M60_002197"
     CDS             complement(10189..11013)
                     /gene="pstA"
                     /locus_tag="L3M60_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003460575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBN6226857.1"
                     /translation="MRKFKENLLKSLVYLSALFTIVSLVVIVGFIFIKGIGKMNFNFL
                     FSNYSASGDGGILPMIITTLYTVLVSIVVATPIGILAAIYLQEYAKKGKAVTAIRFAT
                     ESLSGIPSIVYGLFGGIFFVVTLKMGYSIVAGSLTVAIIILPVIIRTTEEALKTVPQA
                     YRESSLALGATKFQTLYKVVLPSAIPGILSGVILSIGRIIGESAAILLTAGTVAKMPG
                     GIFDSARTLTVHSYLLTKESGDIATAASIGIVLIVIVLALNMLARFVAKKLNKANY"
     gene            complement(11015..11932)
                     /gene="pstC"
                     /locus_tag="L3M60_002198"
     CDS             complement(11015..11932)
                     /gene="pstC"
                     /locus_tag="L3M60_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HBN6226858.1"
                     /translation="MIVKSRLVGLEKNDNNGNKTKYVIEKLAKNIFLLSALVAVASLL
                     LIIGFVFYKGLRPFIFEGYSFIDFLTGANWVPSANKFGISSMIVASIFATLGALVIGV
                     PVGILTSVFIAEVAPKRLAKIMSPAVELLAGIPSVLYGVFGLAVIVPMIQSIFNLPKG
                     QSLLAIVIVLAVMMLPTIISVSETAIRAVPKAYKEGSLALGASKIETIFKVVLPAAKS
                     GVLAAVVLGVGRALGETMAVILVAGNSPVMPTAITDSVRPLTTNIALEMGYAFGTHQE
                     MLFATGVVLFTFILILNLVLNKLSNKAVN"
     gene            complement(12879..13559)
                     /locus_tag="L3M60_002199"
     CDS             complement(12879..13559)
                     /locus_tag="L3M60_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226859.1"
                     /translation="MQTKILIIDGDKDNCQKLKKFMEEKGISIDLAYNCEEAIGKIFS
                     NKYDLIFLEIILTDGDGWTLCKKIRNVTTCPIVYITYINEDQSILNALNSGGDDYLIK
                     PLNLEILYAKVKAILRRMNSYVNNNENNTKLEEYNRTSGVIKLEDKIIKLTPTENKLL
                     NFFIENPEKTLTTKEIYEHVWMNEYLEDNYSVVVAVNGLRKKIEKDYKNPQKIITIRG
                     AGYYFNKE"
     gene            complement(13609..15624)
                     /locus_tag="L3M60_002200"
     CDS             complement(13609..15624)
                     /locus_tag="L3M60_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBN6226860.1"
                     /translation="MKKYNILKGTLIVLIVSLLAITGLNLLYKYDNKYTHKSIRASNG
                     ILNIDNETFTKSKLVFLIDDWEFYNHKIFKPNDFIEQDIKPEYVFIGQYPDFSMRKGN
                     QSPYGNATYRMRIKNDGKNRLLSLELPEIFSASEVWINGEMVSKLGDVGTEKYRPKIK
                     NLVVSFIAKKDTEIIINVSNFSHYYSGLYYPPALGETKDISNMMFYRVLFYSIICFTT
                     FAIAIFSLSVWFLSNKSKLYLYFGCMCIFFAIHVSYPFVHLLGLPLVNFTYAVEDSSY
                     FIAVLCIICINGSISKIEENKLYRFLILPLGITMSIMSIVIPIILLPYDMYFVNIYGK
                     LIDIYKYIVFIYILMSSLVSIYKKTCSEHISSYILVSVNTVFGISILFDAITSNRFEP
                     IFTGWQTEYCGFIIVILFSVMMINKNRIIIKENEKLTRNLEHEVEVRTNELTTLLNER
                     KKFLADVAHDLKAPVSAIQAFIDLIKVGNIHVDEETRTYLYAINQKSNEVQNRVRSLQ
                     EFTSQDKSIGISEKICLNDFIKEIYENNLPDTQACGVNFETYILEKKIYAKINKEMLI
                     RAFENLIYNALTFTPFDGKIILSMDEEDGFAIIKISDNGKGIPPENIPKVFDRFFTER
                     DNNNPKGQGLGLYIVKSIIREHGGEIFVDSELGKGTVFTIKLSLCFL"
     gene            complement(15696..16373)
                     /locus_tag="L3M60_002201"
     CDS             complement(15696..16373)
                     /locus_tag="L3M60_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226861.1"
                     /translation="MYNLLVVDDDVDILNINQIYFSNIGFNVYTSETAKDAMDIVESI
                     VLDCIILDISLPDIDGYKMCNDIKEKVNIPIIFLSNYEHEEEKVRGFLAGGDDYITKP
                     YSLKELELRIYARMRQYKNITSVPNVLKFSALVINVNSRKVTFDGESIDLTTMEFEIL
                     LFLAENKEQVFSKIEIYNQVWKMPDIGDQHTVQVHIAQMRKKINSLSREHQYIQTVWG
                     KGYKFVP"
BASE COUNT         5405 a         2941 c         1641 g         6593 t
ORIGIN      
        1 ttaagataaa atttttagat ttatatcaca atttagaatt atttgtaaaa aaggagatta
       61 tcttttttat tagacaatcc ccttgtatat tttattaata tttatctatg attaagcatc
      121 aattttattt tatacttttt gattttaata ctatatatct aagtcttatt tagggggaac
      181 aatcatttat atgctataaa aatgctaatt tgaataaact tgtcttataa tcagttttac
      241 aaaatacacc attctacaaa gtatgaatca aaaggcgaaa aaatactaaa aataataaat
      301 caccatcaat tttaatcaat atatgaaaaa cttgcactaa gtatacataa aaactaatat
      361 aaatattatc ttaacaacta atacttatac cttcatatat tatatcatat ttcaataaat
      421 aaattgcata aaaggaaatc ttttcataga attaaactat ttattagtcc actactataa
      481 gtttaataaa ctataagata tcactttaat atttatattg tacttcctta aatattaata
      541 taattaatta aagtatacat agtaaaatga aacaaatatc tatatagctt tataaatagt
      601 cataaattta aaaaagtaag catattcgaa aaaggcatat actataaata aaaatcaatc
      661 atagcatatt aataatcaag ctactataat gtaatttcta catatacata ttaaacaata
      721 ttaaaagtct ataaactact attttacata ataaaactgt cttaatataa ataatctttt
      781 atatattaag acagttttaa tcaatagccc tgtgatgaca ttaaatattt tttacgatat
      841 tttcacttac ctttttaaaa ttaatacatt atatcacata acaagcttgt taatgctact
      901 tttaaatctt taagtctata cttacttata ggtataaatt gttcattaat agtaatcata
      961 ttatccctaa gatcttctat tcttcttaaa ttaacaataa aacttgtatg acatctaaaa
     1021 aagccatttt cacacagtaa tttctctgct tgtttcatgc tcatattagc tttataatct
     1081 ctacctattg tatgtacaac taaatctctc tttacagttt caacatataa aatagaatct
     1141 atcagtattc tattaaatcc ttttttacta tttaaaacta tataacgctc cttctttctt
     1201 tgaaggttat caatacaaag atttacaccc attgcaaagt tactatactc tataggcttt
     1261 actaaatatc tataagcacg aacctcataa ccctggggag catactctga aaaagctgtt
     1321 gtaaatataa tttctacttt ttcatcaaac tttcttattt ttttagccac ctcaattcca
     1381 ttgatatctt tcatttggat atctaaaaat ataatatcaa ttttttctgg gtatttggat
     1441 attaaatctt ccccacagga aaattccagt atttgataat ttagacatct ttcagacaaa
     1501 agtttttctg taaactcttt tagtatgctt ctttgtattt tttcgtcatc acatataact
     1561 accctgaaca taatatcacc tccaaatcta aaatatcatt tagtttactt tatttcaatt
     1621 tccaaactgt acttactagc tttcaaattt aaatttcttt ataaatttaa attgtcgatt
     1681 aaactaccta tagaaattct ctttaatatt taaacttaaa ttacgataaa ttcctatact
     1741 tttctagaga tattatgtaa aaaaatacag taaaataaat ataatatgta tttattaccc
     1801 tatattaaac atcataatat atcaaatttt tattgcttat aaattttaag atataaaatt
     1861 catatattca taacagaaaa taaaaatata acacatctgt aaagaattat aacatcatat
     1921 ttgtatatat ataaagaaaa acatacaaaa agtatagaat atatactttt tgtatgtttt
     1981 atttcttctt atatttattt tattttagat tctaaatttg ttaatctttc ctcaacagat
     2041 ttaatcatct cttcaaactt ttcttttttt gctttttctt cttcaattaa actttcagga
     2101 gcttttgcta agaaaccttg atttgcaagc tttccattaa ctctctttat ttctccttct
     2161 aactttttct tttctttaga taatctctca atttcttttt caaaatcaac taattcatta
     2221 agaggtataa atatttcaac tccatcaata actactccaa ctgcatcttc tggaacatta
     2281 gatttatctt ttgctatctc tacatttgaa gcagatgcta aagttataaa ataatctttg
     2341 ccaagctcaa tagcttctat tttttcttca cttgggatta ttatcaattt agctttttta
     2401 gaaggtggta cattcatttc tgctctcaca tttcttatac ttcttatacc ttccatcaat
     2461 aagttcatca tatcttcttc ttcagccatg ttatcttctt cattatattt tggccattca
     2521 gatactatta tacatccttc aacagtagga agatgagtat atatttcctc tgttataaat
     2581 ggcatatatg ggtgtaataa ttttaatatc ttttcaagaa cataagttaa tgtatataat
     2641 gctgcagatt tagcgtttgt atcttccccg tacaatctag gtttaaccat ttcgatatac
     2701 caatcacagt attctgacca agtaaaatcg tatatctttt gaagtgctat tcctaagtca
     2761 aacttatcca tgttattagt tgcttctttt acaatattat ttgctcttga tattatccat
     2821 ttatcagcta gagttaagtc ctctttaaca ctttctctag tcatgttttt aattatatct
     2881 tcatcaatat tcataaatac aaatcttgat gcattccaca acttattcgc aaagtttcta
     2941 gcaaattcta ctctttccat atagaatctc atatcatttc ctggggaatt tccagttgtt
     3001 aatgtaaatc ttagtgcatc agctccatat tgctcgatta tctccaatgg gtcgatacca
     3061 tttcctaaag atttactcat ttttctaccc tgagaatctc ttactaatcc atgaaccaaa
     3121 acatggtcaa atggcttttc attcatacag aacatacctg caaatgccat tctaactacc
     3181 cagaagaata ttatatcgta tcctgtaaca agtacacttg ttggataata ataatctaaa
     3241 gcttcagtct tatttggcca ccctagagta gagaaaggcc ataatgcaga actgaaccaa
     3301 gtatctagtg catcttcatc ctgtttaaaa tgagtgcttc cacatttagg acacttatct
     3361 ggcatttttc tagcaactac aatttctcca cactcttgac aataataagc tggtatttga
     3421 tgaccccacc ataattgtct tgatatacac caatctctta tattttctag ccattgtaaa
     3481 taagttttat caaatttttc tggaacaaat tctaattctc cttttttaag aatatctata
     3541 gctggttttg caagttcttc catcttaaca aaccattgct ctgataatct aggctcaact
     3601 actgtatggc atctataaca agttcctaca ttatgattat gctcttttat cttaattaga
     3661 taaccttgtt cttttaagtc tgctacgata gcttttctac actcaaatct gtccatacct
     3721 tcatattttc cacaaacttc attcatagtt ccatcttcat tcatagtatt taactgctca
     3781 agattatgtc ttagtcctac ttcaaaatca ttagggtcat gtgctggagt catcttaact
     3841 gcaccagttc caaactctaa atcaacataa ctatctgcaa ctattggaag ttctcttcct
     3901 accaatggaa gtattgctgt ttttcctatt aaatgtttat atctatcatc ctctggattt
     3961 actgctatac cagtatcacc tatcattgtc tcaggtctag ttgtagctat ttctaagaag
     4021 tcttcactat cttttagtgg atactttata tgataaaaat ttccgtcatg ttcttcatgt
     4081 tcaacttctg catctgataa agtagtttta caatctggac accagtttat tattctgttt
     4141 cctctgtata tgtgaccttt ttcatatagg cttacaaaaa attctataac tgcttcatta
     4201 cacccttcat ccatagtaaa tctttcttta tcccaatcgc aagaatcacc taattgttta
     4261 agctggttag atatttttcc tccaaattca tctttccact cccaggctct ctttaaaaat
     4321 tcttctctac ctatttcata ctttgtctta ccttcttgtt tttttattct ttcaacaact
     4381 ttaacttctg ttgctataga agcatggtca gttcctggaa gccaaaaagc ttcatatcca
     4441 tccatacgtt tccatctaat taatatatct tgaagtgtat ggtcaagagc gtgtcccata
     4501 tgcaattgcc ctgttatatt tggtggtggc atcattattg taaatggttt tttatctgga
     4561 tttggtttag atttaaaata cccttcatcc atccattttt tatatagcct agcctcaaat
     4621 tcttttgggt tataagtttt tgataaattc gtattttcca ttgtcctgcc tcctgtattt
     4681 tataaataaa tttttaaata tctttagaaa taaaaaagag ctcttcatcc tcttcaaaaa
     4741 ggacgaaaag ctcgcgttac caccttaatt ccaaagctta aaatacatta acgtataatt
     4801 aaattatttg ttaatattat tttataaact ttgactctca aagaatttaa cggttctaac
     4861 cgtctaaact tactcatagt tcagtttaga aactccaaag ctacattcag taaatcttat
     4921 acttagaaaa tcttacagcc agtgaatttt cctctctttt agtagtattt acttactcct
     4981 cttcttctca gtctttaata ttacatatta ttatatacaa atattattct ctttgtcaat
     5041 tatttttatt ttattttttc tgaattttct taattatatt tattttcttt tatactaatt
     5101 gttatatttt aaaatatatt tttgaagtta ttttgtctta taatgtcaat aattgcaaga
     5161 ttgttttatt ttaattttaa tactttctca ttaactttat attatattat ccttaaagta
     5221 tacctctttt ttgtattttt tatattatat gttttacttt ataaataatt tactttattt
     5281 tttaccatat ttaaatttat tataagtttt cttgccagaa atttttaggt gatatatttt
     5341 ctgtaattgg caatattgta tatcctctct cttttaagat tcttataata ttatttaaag
     5401 catttaaact ttgctctttc tcatgaatta atactactaa ttcatctctt tttcttccat
     5461 aatataatat attacttact atctgacttg tagaagactt ccaatcctgt gtatccaaat
     5521 tccagtccca aattaaataa tcatttgcca taagtttatc atatgaacct tctggcatat
     5581 atggtttgct tccatatggt aatcttatta attttgaact ttgaccagtt atttcttgta
     5641 gagtattatt gcatgtatta aattccccaa ctgttacttc tgggctttta tataacttat
     5701 gtatatcatg acttacacta tgaaaaccta ttgaattttc ctcttccaaa actcttctta
     5761 cttcttcctt gtgatttttc atattttgat taagcatgaa aaatgtcgct ttgacatcat
     5821 tttcttttaa aatatcaagc agtgcatttg tatattttga tggaccatca tctattgtta
     5881 tgtatgctac tttctcagaa gtagctcctg gaccttttgt ttcttcttga gatgctttat
     5941 atatgatttc tcctaaagta ttactttgag aaagtaattc cagtgtactt tcaccttttg
     6001 aacttatatt ttcaactgga acattattaa ttttttcctc caataatgtt ccagttaaaa
     6061 ttaaaacagc tacaagaaat attcttacaa atacatgaaa aacagtctta ttatctttaa
     6121 ttttcatccc ccatatctcc cttttataaa agcataagtt taagacttaa aaagttattt
     6181 tttatattta ttctctttcc tattactttt tatatctcta ctaaatattt ttttaacctt
     6241 ttcttcttct ttttctccta gagattccac tcttttgatt cccaaagaag ttaaaaattg
     6301 taatacattt aaattatcaa atggagtttc catattcgta ctttcactat attcatcaac
     6361 attcttagta ccttcttcca cacttaaaat acgcttagga cctcctacac agcctcctac
     6421 acaccccatt ccttctataa atgtagcgtc tatttcttta tttagaactt tctttagacc
     6481 gtccatgcac tcttttgttc cttcaaatgc tttttcttca aattttatat ttttgtcaat
     6541 tcttctaact gataacttaa ctgctttact tacacctcct gttctagcat aaactctacc
     6601 tgaaagggat gcctctactc tattactctc atccattatt gctggattta actctaatgc
     6661 atcaaatatt tcttgtaact ctttaaatgt aagcacaaaa tcaactgcat cttttatgtc
     6721 ttctaacatt gcttctttct tttttgccat acaaggacct atgaatacaa ctttagcatc
     6781 tggattcaaa atttttacag ctctcccaca agcaatcata ggagaaacgg atggtgatac
     6841 attctccaat atctgaggaa agctattttg tatcaaacta acccaaatag gacagcaaca
     6901 actagttata aagcatcctt cttcgtcctt tttcatatgt tcataataat catatgcttc
     6961 ttttgcagta agcatatctg ctgccaaagc aacttctatc atatcttcaa atcctatctt
     7021 ctttaatgca gttcttaatt taccagggct tacatctttc ccaaactgtc ctataaaagc
     7081 tggtgcaact attgcatata cagggctctt tatatctttt aacaatttta tcattggtat
     7141 aaattctatt ttatctgaaa ttgcacccaa cttacatctt gatacacaat caccacaagc
     7201 actacataca tcttcctcat tacaattaat atgcttactt cttatcttac atgactcatc
     7261 ttccttatta ttttcatcac aatatttgca agcatctgat attatggata ttattggtct
     7321 tttgacttca tctaaattta cagcttcatg tagtgaatct tttaaatttt tatctgtatc
     7381 tcctgtcaca tccaatccca tagttactct aattaaattt accatagtat ctttatcgaa
     7441 ttttccacta aaataattat ttaaatcatc ttttaaatct tcatttaaaa ttttattagg
     7501 taagttttca atatcctcta tatcatcatt ccacactgct tttacaagtt cgccaaaaag
     7561 cttcatttta tatatttcta atattttaga attatcattt ttagtattca taaaaaatag
     7621 tacctccata aattaaactt atatatgttt aatatttaca aagatactat tttttattta
     7681 ttatttatct actattatta atatgattta tttaactttc tcagcaattc tatttcaatc
     7741 attattttca ttagatgata gttgaataca ggactttttt cttcatctat attccagtct
     7801 agcttatcta cattatatgt tttcttaagg tatttatatg tatgctcttt caaataaagc
     7861 ttattttcaa gtaactctat agactgcata tgaaaatgta tttctctaca tatccttaag
     7921 gcgttctcaa ttttttctat attagtttca ctcaaactat aatccaactc atcaataagt
     7981 tttgaatata tttcttctag tttttctact tcttcttcaa acttctctac atttaaattt
     8041 tcttttctca taattttatc ttcaataagc aacatcgaaa gttcttctat ctttttaaat
     8101 tcatctatga cgctatttag ataatttggt ctagcaacat aataattaac aagaacccca
     8161 aacaaaacac ccacagatgt atctaacacc ctatgtaaag aataatacac agggtcacta
     8221 tttcctacac ctaaatgtat agccagaaag gttacacagg caactactac tgactttatt
     8281 ttaaacagat tgcataaata tattgagcac attactccta gaccacataa aaaagggtct
     8341 ccagctttaa tgatggcaaa tatgaatcct attataccac ctaaaatagt accttccact
     8401 ctattgattc ctgtctttaa agaacctttt accgtatctt gaacagaaat tatacatgca
     8461 actaaagaat aaaacatatt ctctactaga tagtcaccaa gaatcataca taaagtgaca
     8521 ctaattcctg tttttatagt cctcataccc atcttttgaa gtttcaaaat tccacctcct
     8581 aaaaagcacc ttatataaaa tacgcatcct catatttaat atgttcctag tatatcacaa
     8641 ttttttaaat ttacccatta atcatatttt tttatcaatt ctttcttaat ttagacaaaa
     8701 aaaaactgct ttaaagcagt ttttttatcc tatctcaacc attttaccat caattgcaaa
     8761 aattatcttc tcgcatacat ttgttatatg gtctccaatt ctttcaaggt atcttcctat
     8821 aagtagaagt tttacccctt ggtctatagt atttgaattt tcatgcatta tttttaaaca
     8881 atcaatttgt acttgttcat acaatccatc tacaatatca tctttagttc caacatcata
     8941 agctatactt gcatcaaata agattaaagc ttctctagtc tgagacatca tagacttaca
     9001 ttcttttgcc atcttaggaa tatctatcag ctcttttata taagcctctc cacctatctt
     9061 caacatttct ctagctatat ttgctgcata atctcctatt ctttctaact ctatcgatat
     9121 attacctaat gaataaataa atcttaagtc ccttgccatt ggttgcttta atactattaa
     9181 ttctatactc ttatctctta tttcttctct taatttatct atttcttcat catcttctac
     9241 tacttttctt gcaccctcta aatccttctc tatcatacaa gtcacagact tttctattgc
     9301 ttcttcacat ttttctatca taagtactgt ttgttctttt aaagtatcaa tacttatatc
     9361 taaactatta ttgaccattg tctaactcct ttatagttat atattttaac caaatctacc
     9421 tgttatatag tcttctgttc ttttatctac aggagtggta aatatagttt ttgtatcgct
     9481 aaattctata acctctccat ttaggaaaaa agctgtttca tctgatatac gagcagcttg
     9541 ttgcatatta tgtgtaacta tgactattgt gtagtctttt tttaaatcct ctataagttc
     9601 ttctactttt aaggttgaaa ttgggtctaa agcagatgta ggttcatcca taagaataac
     9661 ttctggtctc atagcaattg ctcttgctat acatatacgt tgttgttgac caccagaaag
     9721 tcctaatgca gatgatttaa gcctgtcttt aacctcatcc caaatagctg caccttttaa
     9781 gctttcttct acaatcttat ctaattgctt cttatctctt agcccatgta tacgaggacc
     9841 atatgccaca ttatcatata tactcatagg aaatggattt ggcttttgaa ataccattcc
     9901 tactttagta cgaagattta taacatcatc tgaagtatat atatcctctc catcaacaga
     9961 aatacttcct ttaattgtta catcttcgat tagatcattc attctattta aagttcttat
    10021 aaaagttgat tttccgcatc cagatgggcc tattaaagca gtaaccttat tctcttttat
    10081 atccatattt attttcttta atgcttgctt atcaccataa aataaatcca aatcttttac
    10141 actcattttt atcttatcga ttaattccat tattcacacc tcttaaattt aataattagc
    10201 cttatttaat ttcttagcaa cgaatcttgc caacatgttt agtgctaata caattactat
    10261 taaaacaatt cctatactgg ctgctgttgc tatatctcca ctctctttag tcagtaagta
    10321 tgagtgtaca gttaatgttc ttgcactatc aaatattcca cctggcatct tagctacagt
    10381 accggctgta agtaatattg cagctgattc tccaattata cgaccaattg ataagattac
    10441 tccagataag attcctggta ttgcacttgg aagtactacc ttatataaag tttgaaattt
    10501 tgtagctcct aatgctaatg atgattctct ataagcttga ggaactgttt ttaaagcttc
    10561 ttctgttgta cgaattatta ctggtaatat tattattgct actgtaagtg aacctgcaac
    10621 tatggaatat cccattttta atgtaactac aaagaaaatt cctccaaata atccatatac
    10681 tatagatggt atccctgata aactttcagt tgcaaatctt atagctgtaa ctgcttttcc
    10741 ttttttagca tattcttgta aatatatagc agccaatatt cctataggag ttgctacaac
    10801 tatagaaact aatactgtat acaatgttgt aattatcatt ggaagtatac caccatcacc
    10861 tgatgctgaa taattgctaa ataagaaatt aaaattcatt tttccaattc cctttataaa
    10921 tataaatccc actataacta ctaatgatac tattgtaaat agtgctgata gatatactaa
    10981 tgacttcaat agattctctt tgaactttct catcttaatt taccgcctta tttgaaagtt
    11041 tatttaaaac taaatttaaa attaatataa atgtaaacaa tactactcca gttgcaaata
    11101 gcatttcttg atgtgtacca aatgcgtaac ccatttcaag tgctatatta gttgtaagtg
    11161 gtcttacact atctgttatt gcagttggca taactggtga atttcctgca acaagtatta
    11221 ccgccattgt ttctcctaaa gctcttccta ctcctaacac tactgcagct aatactccag
    11281 attttgcagc aggtaataca actttgaata ttgtttctat ttttgatgca ccaagtgcta
    11341 atgaaccttc tttatatgct tttggtactg ctcttattgc tgtttctgat acagatatta
    11401 ttgttggtaa catcattact gctaatacta ttactattgc taacaaactt tgaccttttg
    11461 gaagattaaa tatactttgt atcattggaa ctatcactgc aagaccaaat acaccataca
    11521 atactgaagg tatacctgct aaaagttcaa ctgctggtga cattattttt gctaatcttt
    11581 taggtgctac ttctgctata aatacagagg ttaatatacc aacaggtact cctatcacaa
    11641 gtgctccaag tgtcgcaaat attgaagcta ctatcataga agatattcca aatttattgg
    11701 cagaaggaac ccaatttgca ccagttaaaa agtctataaa tgaatatcct tcaaatataa
    11761 atggtcttaa tcctttataa aatacgaatc ctattattaa aagtaaactt gcaactgcta
    11821 ctaaagcact aagtagaaaa atattttttg ctaacttttc aattacgtat tttgtcttat
    11881 tgccattatt gtcatttttt tctaaaccta ctaatctaga ctttactatc ataatatcct
    11941 cccattaaaa tatacttatc gattttcatt ctgtattcaa aatttaaatt tttacaattc
    12001 gtcttcatca aatttcctgt acttttattc tacttttttt aagttaaatc tatattagga
    12061 ttaagttaaa caagtgtaaa attatattca cttttttaac attaatttta cttttaaaac
    12121 tatatttgac atttacttta catataatgg tgttttgcta tattaaaata gcagatatag
    12181 gtaataatac gctttatgtg taatttatta cctatatctg ctattaagat ttgttaacta
    12241 aatttaactt ttatactaca tatataattg ttatctccta ttgtctatat cactattatt
    12301 ttcataaaat tgttatgata cttgatgttt acaataagta cgaatagaca aaaatataaa
    12361 aataggtttt attaggctta tgataaaaaa caacatttga aatgatattt tcaaacattg
    12421 agcaccaaaa gttaaatcat aaattgccaa ataaatagaa atacatacaa caaacattac
    12481 tataaatata gcaaatagag ctaatatttt agtaaatgct aagataagtg tatccatgtt
    12541 tagtacaatg aagtatgcta tatgatagtg tctgatattc acttcacaaa aaaaggtaga
    12601 tgcaaagatt gcaacacata caattaataa tatgaatata ttaaggataa aaatgaataa
    12661 tatatagatt tatatataca atttgtttat cttttcaaat gtaacccata aaataatggt
    12721 gcaggtaaac atctttttaa gtaataaaac tgactgtaat ttaactgata aagtctagta
    12781 aataggcttt atcaattaaa ttacagtcag ctattttata ataatttagt ttagatgtcg
    12841 ttaaaatttc acatttataa caagtattaa tatatttatt attccttatt aaaataatat
    12901 cctgctcctc ttattgttat aattttttga ggatttttat aatctttctc tattttcttt
    12961 cttaatccat tcactgcaac taccacacta tagttatcct ctaaatactc attcatccat
    13021 acatgttcat atatctcttt agtagtcaaa gtcttttcag gattctctat aaaaaagttt
    13081 aacaacttat tttctgttgg agtcaatttt attattttat cttctaactt tattactcca
    13141 ctagttctat tatattcctc taattttgta ttattttcat tattatttac atatgagttc
    13201 attcttctta aaatagcttt tacttttgca tataatattt ctaaatttag aggctttatt
    13261 aagtaatcat ctccaccaga atttaaggca tttaaaatgc tttggtcttc atttatgtaa
    13321 gtaatataaa caattggaca agtagttaca tttctaattt ttttacaaag agtccaccca
    13381 tcaccatcag ttaggattat ttctaaaaat attaaatcat atttattgct aaatattttt
    13441 ccaatagctt cttcacagtt atatgctaaa tctatactta ttcctttttc ttccataaat
    13501 ttttttaatt tctggcaatt atccttgtct ccatctatta tcaatatctt agtttgcatt
    13561 ataatcactc ctaaatatca ttattctttt atattaaata agctattatt ataaaaaaca
    13621 tagacttaat tttattgtaa aaacagttcc ttttcctagt tccgaatcta caaagatttc
    13681 tccaccatgc tctcttataa tggatttgac aatatataac cctaaccctt gcccttttgg
    13741 attattgtta tctctttctg taaagaatct atcaaatacc tttggtatat tttctggagg
    13801 tatccctttt ccattatcag aaattttaat aattgcaaat ccatcttctt catccattga
    13861 caaaattatc ttcccatcaa atggagtaaa ggttaaagca ttataaatta aattctcaaa
    13921 cgctcttata agcatctctt tatttatctt tgcatatatt tttttctcca atatgtaagt
    13981 ttcaaagttt acaccacatg cttgtgtatc tggcaagtta ttttcatata tctctttaat
    14041 aaaatcattc agacatattt tttcactaat accaatactt ttatcttgtg atgtaaattc
    14101 ttgcaaacta cgtactctat tttgtacttc atttgacttt tgatttatag cataaaggta
    14161 ggttcttgtc tcttcatcta catgaatatt accaaccttt atcaagtcta taaatgcttg
    14221 tatagccgaa acaggtgcct ttaaatcatg tgctacatct gccaaaaatt ttttacgttc
    14281 atttaacaat gttgtaagtt cattagttct cacttcaact tcatgttcaa gatttcttgt
    14341 aagtttttca ttctctttta ttattattct attcttattt atcatcataa cagaaaataa
    14401 aataacaatt ataaacccac aatattctgt ttgccaccct gtaaatatag gttcaaacct
    14461 gttgctagta attgcatcaa ataatatgct aattccaaaa acagtattta ctgaaactaa
    14521 aatataagaa cttatatgtt cagagcatgt ctttttatat attgaaacca gtgaagacat
    14581 taatatatat ataaatacaa tatatttata tatgtctatt aatttcccat atatgttgac
    14641 aaagtacata tcatatggaa gaagaataat tggtatcact atagacatga tactcattgt
    14701 aattcctaat ggtaaaatta aaaatctata caatttattt tcttctattt tggatatact
    14761 tccatttata caaataatac ataaaactgc tataaaatat gatgaatctt caactgcata
    14821 tgtaaagttt actaaaggta gtcctaacaa atgtacaaag ggataagata catgtatggc
    14881 aaagaaaata cacatacacc caaaatataa gtaaagttta cttttgtttg aaagaaacca
    14941 tacagacaac gaaaatatag ctattgcaaa agttgtgaaa caaattatac tataaaaaag
    15001 tactctataa aacatcatat tacttatatc ctttgtttct cctaatgcag gtggataata
    15061 caaaccgcta taataatgtg aaaaattcga tacattgatt ataatttctg tgtctttctt
    15121 tgcaataaaa cttactacta aatttttaat ttttggtcta tatttttctg ttcctacatc
    15181 tcctaattta gaaaccattt ctccatttat ccatacttca cttgcagaaa aaatctcagg
    15241 aagctccaaa cttagtaacc tattcttacc atcatttttt attctcatac gataagtagc
    15301 attcccatat ggtgattgat tcccttttct catagaaaaa tctggatatt gacctataaa
    15361 aacatattct ggttttatat cttgctctat aaaatcattc ggcttaaata ttttatgatt
    15421 ataaaactcc cagtcatcta tcaaaaatac aagtttactt ttagtgaaag tctcattatc
    15481 tatatttaaa attccattac ttgctctaat agacttgtgc gtatatttgt tatcatattt
    15541 atacagaaga tttaatcctg ttattgccaa cagagagact atcaatacaa tgagagttcc
    15601 ttttagtata ttatactttt tcattgcatc aaccacatct ctttcatagt agtgttaaat
    15661 aaaataagaa ctatttatta attacagcat ctcacctatg gtacaaactt atatcctttt
    15721 ccccatacag tttgaatata ttgatgctcc ctgcttaaag aattaatttt ttttctcatc
    15781 tgtgcaatat gaacctgtac tgtatgttga tccccaatat caggcatttt ccacacttgg
    15841 ttatatattt ctatcttcga gaatacttgc tctttgtttt cagctagaaa caacaatatt
    15901 tcaaattcca tagttgttaa atctatactt tctccatcaa aagttacttt tcttgaattt
    15961 acatttatta ctaacgctga aaactttaac acattaggaa ctgatgtaat atttttgtac
    16021 tgcctcatac gagcatatat tctaagttca agttctttta aactataagg ttttgttata
    16081 taatcatctc ctccagctaa aaatccccga actttttctt cttcatgctc ataattagat
    16141 aaaaatatta taggtatgtt aaccttttct tttatatcat tgcacatttt atatccatct
    16201 atatctggca atgaaatatc caaaattata caatctaaaa ctatactctc tactatatcc
    16261 atagcatctt tagcagtctc tgatgtatat acattaaaac ctatattaga aaaataaatt
    16321 tgatttatgt ttaaaatatc aacatcatca tcaacaacca gtaaattata cattacacca
    16381 ctccattcaa aggtatttta atcatagtct caagttttat aattattaca aattataatg
    16441 ttttattttc atatataact agtattatta ttttataata tctttacaaa ctagactata
    16501 atacttaagt atttttgaat aattcttgaa tattgtatta aacattaaga ctcattatta
    16561 aatactaatt aaagtctccg
//
LOCUS       DAFNNB010000081        16532 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0082, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000081 DAFNNB010000000
VERSION     DAFNNB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16532
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0082"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            75..1121
                     /gene="selD"
                     /locus_tag="L3M60_002202"
     CDS             75..1121
                     /gene="selD"
                     /locus_tag="L3M60_002202"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:123..125,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBN6226862.1"
                     /translation="MKKEQKKLTEMTTAGGUAAKIGPEVLASVLSQLPKNDNIENLLV
                     GLDTADDAAVYKLNDDMALIQTLDFFTPMVDDPYVFGQIAASNSLSDVYAMGGKPLVA
                     MNIVCFPSCHDMDVLAEILKGGFAKVKESGALLVGGHTVDDKEPKYGLSVSGIVNPNK
                     VLSNATAKPGDKLIITKPIGVGVLNTAMKEGMVEQHIADKVIEIMIHLNKYAAMSFDK
                     FDVNSVTDITGFGLLGHTLEMAKASEVSIEIESKHVPIIEGAIEMAQMGIIPAGMYKN
                     MHYVSKDVEIVGNVEVAVQDILYDPQTSGGLLISVKEELAEELVKDMKLNGAIEAKII
                     GSVVPKGEKYITVL"
     gene            1139..2551
                     /locus_tag="L3M60_002203"
     CDS             1139..2551
                     /locus_tag="L3M60_002203"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBN6226863.1"
                     /translation="MIILSKRELFAMLPSVDEVLSDNRIVEIINEYPRSLVLESVREV
                     IDLKRQFILRLKEDISNSVTIEFGEIIESAIERVKLNYSLSLKKVINATGTVIHTNLG
                     RSLLSEDIKDELWCAASRYSNLEYDLDNGERGSRYSHLTSTIKRLTGAEDVLVVNNNA
                     AAVLLVLSTMAKGGEAIVSRGELVEVGGSFRIPSIMALSGAELVEVGSTNKTHLKDYK
                     EAITEDTNVLMKVHTSNYRIMGFTESVSIEELVNLGKKYKLPVIEDLGSGVFIDLSKY
                     GLSYEPTVLDSIRQGADVVTFSGDKMLGGPQAGIIVGKKEYIEKMKKNQLTRALRVDK
                     LTICALEATLRMYLDETKAIENIPTLKMLTYKIEELEVKANKLFEKITALNLNANINI
                     EDGFSQVGGGSMPLETISTKVIAITPEHMNVSSLEKKLRLSEAHIIARVYDNKYVLDV
                     RTIFDDEFDVIVDELRKAFN"
     gene            2665..4548
                     /gene="selB"
                     /locus_tag="L3M60_002204"
     CDS             2665..4548
                     /gene="selB"
                     /locus_tag="L3M60_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBN6226864.1"
                     /translation="MKNVVIGTAGHIDHGKTTLIKALTGRETDTLDEEKKRGISINLG
                     FTYFDLPNNRRAGIVDVPGHEKFIKNMLAGASGIDIVLLVVAADEGVMPQTVEHLDIL
                     SFLNIKNGIVVLTKSDTVDDEFRELVKEDICEKIKGTFLEDAEIIEVDSISKRGIDTL
                     IEKIDKMSDDIEDKNESSPARLNIDRVFSIKGFGTVITGTLIEGKISIEDDLVIYPQV
                     LKTKIRSIQVHGENKDTAYAGQRTAINISNVKVDDIKRGDVLAAPNSLEESMMLDVKL
                     SLVNHLDKGLKHWDRLRLYHGTREILCRAVPLDKELIENGESGYVQLRLEESIVSKKG
                     DTFVVRRYSPMETIGGGVIIDPSPKKHKKFDEKVIEALKIKEKGELKDIIEEYLKRNL
                     KNYPNIKEIMSYSGAHEEDVKKALETLISGDKVFIIGNMYMHVNQYNKLKENTIKLLS
                     EYHKKYRLRKGMLKEEVRSKIESNFKTREMDILLEKLSTENAIKIENNIVSLLDFEVI
                     LNEKQKEIAKKIEKRLKSCGVSSILTIDEVSEGNHNYAEVLESMIGNKVEKLDDLYIM
                     DKDIYENAKNTLINYIKENKEITLGEYRDLIDSSRKNCMIILENFDRNKITKRVENKR
                     ILF"
     gene            4653..7391
                     /locus_tag="L3M60_002205"
     CDS             4653..7391
                     /locus_tag="L3M60_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation-associated two-component system
                     sensor histidine kinase"
                     /protein_id="HBN6226865.1"
                     /translation="MKSIIPDVKPDIKEREDMSLLFIIMGSVLLLFIIKDTDKYETLL
                     HTIYTIFLVATGVIVFNTTKFKVDSFSTFLGLIFIATGALECIYLFNSLEIKTQFVRE
                     LNITISAITDLFPILGVYLSFKFVKDNKQIYSSIILFIVTVTLTIISLFIISGICEYL
                     GNGTSQYALEVIISIFIIIISIVSGIELNLSSSEGKWEYNEKKIFNRIIVIIILSRVP
                     NLLHIVIDDRNIERILSHIIINVAIYYLYNYIVSKNIKKTALELHDINEELTLKTESL
                     KEKNKKLIYETEKIEELKGILIKRESRLQSTLDVAVNSIVVFSKDGEITYANKSFRNT
                     FGDYKEQDKLTDKIKNFNDLIESINYVFVYEKNVEKLISTSDNKVYQAIFAPLIIASQ
                     TEGVLCVLEDKTKKKEYERKLIEANKRSREFLESIGDGIVVLEGNNKIYVNNACREIF
                     KNNLESIDFSLVCKSEESIEKRYVIDGEVKYVEMSFSQYTNAGTDKTIIVIRDTTSRK
                     ISQIKLEESQSSYSRFIDILPDGICLLKEDLSINYANKSFLDMLAFTNIDDIKDSNIK
                     LLMNANTEEKMKFTDNMAKVLNENRSILLLEYELINSYNDIVEVELSALPFAIYNTRY
                     IMLIIKDLVHKKYSEQAEKELLERFKTDKIKTEFFANMSHELKTPLNVISSSNQLVDS
                     FYRSEKIKDYNNNIKSHVDLVRQSSYRLQRLINNIIDLTKMESGFYTLKLAKYNIVSV
                     IEDLFMNIEEYALRKDIKILFDTELEEINIYIDKVEIERIMLNLLSNCIKFTDNGGWI
                     YVSIHYKIDKVIISVKDTGVGIPQDKLELIFEEFSQVDKTLSRNTEGSGIGLAIVKNL
                     VSLHGGDIEVISEVNKGTEFLISLPMKGFSSEHYTEDKRIYNIQEKIKIEFSDIYY"
     gene            complement(7510..8454)
                     /gene="manA"
                     /locus_tag="L3M60_002206"
     CDS             complement(7510..8454)
                     /gene="manA"
                     /locus_tag="L3M60_002206"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HBN6226866.1"
                     /translation="MEPLFLKPIFMDRIWGGTALKDKFNYEIDSPTTGECWAISSHKN
                     GDCLIENGIYKGKKLSELWDKNRELFGNTPGDKFPLLTKILDANDNLSVQVHPNDEYA
                     KKNENGELGKTECWYVIDCSDDAEIIIGHNAKSHKEFVDMVNNNEWDKLLRKVNIKKG
                     DFFYVPSGTIHAICKGTLILETQQNSDTTYRVYDYDRTDNLGNKRELHVQKSIDVTNV
                     PHINFDTDYKIVSTSDFRCTTFVSNEFFSVYKLDIFGKCTFTHKTPFSLYSVLDGNGK
                     LIHNAVEYNLKKGMHFILPNNFGEFGFEGNLEIICSHI"
     gene            8627..9988
                     /locus_tag="L3M60_002207"
     CDS             8627..9988
                     /locus_tag="L3M60_002207"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HBN6226867.1"
                     /translation="MELFKEEIKKYEKEFLKLLEEWVSIPSFYDRKTVSKDMPFGKGV
                     YDALNWFENLGRENNFKVKNIDNHAVQIEYGNGKEYVDIFGHCDVVNPGEGWDSEPFK
                     LNIIGDKLVARGVSDNKGPMIVNFLALKMIKDLDINLKRKVRLIAGGNEESGFKCIKH
                     YYSKEPYGVCGFTPDAKFPVLNGEKGGAIIKLLSNIDDKSLYISGGIEFNTIPDKVYI
                     KNIEKLGKDNICFDINKISISYDNDNYIVHGKGGHSSKPEKSINPILATIKLLSENID
                     EKWTKDLYKLINQDNINGNLFGLNIEGKCGILSMVPTIINIVDGKLEVVLSARYPEIL
                     SIEEIMKKFNLYMEKNNINKFELIGENLKQANYIDKNSKLVKSLHDIYIKYSGDLKND
                     VRVTSAGSYASEMNNSVIFGCEFPDGSFGNVHGANEFASLDRFITAIGIYAEAIITLC
                     NKI"
     gene            10076..11965
                     /locus_tag="L3M60_002208"
     CDS             10076..11965
                     /locus_tag="L3M60_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6226868.1"
                     /translation="MITKKHAIIVKSLSNKDGYMTSNELAIKLDVSTKTIKRYIADLN
                     SVLSKYDLVIDSSRGIGYKLSGSKNNIARAIKEANKYIDGFLDDSEESRMSNIICMLI
                     NRNYMSIEAMAEELNLSIAAINKLSSKLKRKLEKYDLVIKSKPYYGSYIHGEEINIRQ
                     LITDYAIKLDEKSKVKVFLDDISENDIYCIESILEKHLREKDTIISDKDFNLLLSKII
                     VSVFRSKRGHSNNINLMDTSYRFHNYYFIESLMKEISDKIGFKLIEDEVIYISNYSGV
                     IACKGTQGIKNTSELEERINSVMSSALQDIFLISGSDYTKDDEFMVAISDHIKRFLNR
                     ARANVKSNNPLLHQIKEKFPIAFNLAVFLSNKLETEFNLKLDEDELGYIAIHFAASNE
                     RMKKNTSKKICIVCHYGIGTGQLISEKLKQNISDLSVVGVYPVRYLDMAISQDVDLIV
                     STVELKGYEKPVLYIENIFDDSLIENVNKAFYEKEERRKIISNMFDEKAFFNIKANTK
                     EEVILSLSNKLKERDFIEESSIKSIIDRENISSTEIGNLVAIPHTIVKGDKKSIIGVG
                     ILENPIIWDKQEVQLVFMVFFNTKEKHNFSIFKYLYNFIKDEGGVRGIIKICDFNKLM
                     ALIGN"
     gene            11980..12435
                     /locus_tag="L3M60_002209"
     CDS             11980..12435
                     /locus_tag="L3M60_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6226869.1"
                     /translation="MGTNIFNKEYVFLNVDAKSKVEVLKFISKKAKELNLAEDESLVY
                     EGLMSREEQFTTNLGESIAIPHTKNDAIENPAVVVLKFNEDVVWNEGEDKVKLAISLL
                     MPGKSKENIHLKLLSSLSRKLINKDFKDSLLKSDNVEEISNLINEALGL"
     gene            12493..12810
                     /locus_tag="L3M60_002210"
     CDS             12493..12810
                     /locus_tag="L3M60_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HBN6226870.1"
                     /translation="MNKKLVALCACPMGLAHTFMAAEAIEQAAKALGYEAKVETQGAD
                     GVQNELTRDDILGATMIIHAVAITPEGMERFDGCEVYEVELQEAIKNAEGVIKEIEED
                     LGI"
     gene            12835..14097
                     /locus_tag="L3M60_002211"
     CDS             12835..14097
                     /locus_tag="L3M60_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBN6226871.1"
                     /translation="MAIKKKVINSSGASTTGGNNVGKSTPSSPTQSKGNGKWKEISKH
                     IMTGISYMIPVLVMGGLIGALSQLIPYAILGLDPSVGIVDAMNSGEFTGFKLSLLNIA
                     QLMSNFGFTLFGFAIPLFAAFCANSIGGKTALIAGFIGGYIANKPVGVPQFVDGQWTE
                     VVPVASGFLGAILIAFIIGYFVKWLNKSIKVSHNWLAFKTTFLIPLIASLACMVLMIF
                     IITPFGGLINESMKNFLTAAGAAGEYVYATALAAATAFDLGGPINKAAGFVALGLTTE
                     NVLPITARTIAIVVPPIGLGLTTLLDKKLVGRRVYDRQFYQAGKTSIFLSFMGISEGA
                     IPFALERPGFVIPLNIVGSVIGAITGIILGAIQWFPESAVWAWPLVDNLFGYIIGIAV
                     GAIFIAVGNIFYRNKLIKDGKLVVDYID"
     gene            14202..15266
                     /locus_tag="L3M60_002212"
     CDS             14202..15266
                     /locus_tag="L3M60_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBN6226872.1"
                     /translation="MDKIEKVREYFREYDIDGFLINSPTNKFYVGNLFSSSGYVFITK
                     EAQYIIVDFRYFEEIKRKSSLFNVVLMDKTRTHFDIINDICREQNIKEIGFEGNEVSF
                     DLYRSMSNKLSATLKSVDLSTLRETKNEDEIKYIKKACEIVDATFYHIIDFIKVGMTE
                     KQVENEIVRVIKDLGGQKESFDTIVASGLRGALPHGKASEKVIEYGDFVTFDFGAKYN
                     NYCSDITRTICMGTINKELEEIYNIVRKANEECIRVLRPGMTTGEIDKVARDIIGSYG
                     YANNFGHNLGHGVGIMVHEYPALAPESNEVLKEGMIVTIEPGIYVPSLGGVRIEDDVL
                     ITKDGCMRLTTSTKDLIVVS"
     gene            15320..16498
                     /locus_tag="L3M60_002213"
     CDS             15320..16498
                     /locus_tag="L3M60_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HBN6226873.1"
                     /translation="MYNFDNKPDRVSEKCRKWDLNIIRDKFGDIREDFVPMWIADMDF
                     KIPTEIENKFIEAVRRGVFGYTYCYDEFYDSVIGWQKNMHEVDVEKEWITLTYGTVST
                     LHYVVQAFCKKGDSIILNTPVYDPFESSAKKQGVNVICNTLDVVNDRYYINFDKLEAQ
                     IKENKPKLMMFCTPHNPSGRIWTIEEMTKVATICKENNVILVADEVHAEHIHYGKFNS
                     ILKIEKELLENVILLTSPNKGFNLGGLKTSYSIVINKNIRDKFRNKLKQNSITSPNVF
                     GIIGLITAYNECHEWLNGVNEYIKSNYELLETWVNKYSKIKLMKMESSYLAWMDISGL
                     GISASEFTDRLAIDTGVLLEDGSHFVQDGDKYVRINLGTQKENVIEALNRMDLFLKSL
                     "
BASE COUNT         6705 a         1650 c         2969 g         5208 t
ORIGIN      
        1 acttatatat atggtattat aaatatgtat gtatatgtgt gtatatgtgg aaatttagta
       61 aaggaggcac ttttatgaaa aaagaacaaa aaaaacttac agagatgaca acagcaggtg
      121 gttgagccgc taaaataggg ccagaggttc tggcatcagt tttatctcaa ttgcctaaaa
      181 atgataatat agaaaattta ttagtaggac tagatacagc agatgatgct gcagtttata
      241 aattaaatga tgatatggca cttattcaaa ctttagactt ctttacacca atggtagatg
      301 acccatatgt atttggtcaa attgctgcat caaattcact atcagatgta tatgctatgg
      361 gaggcaaacc tttggttgct atgaatattg tttgttttcc atcatgccat gatatggatg
      421 tacttgcaga aatattaaaa ggcgggtttg ctaaagtgaa agaaagtgga gcacttttgg
      481 ttggagggca tactgtagat gataaagaac ctaaatatgg tctttcagtt tctggaattg
      541 taaatccaaa taaagtttta tcaaatgcta ctgcaaagcc tggtgataag ttaattataa
      601 caaaaccaat tggagtgggt gtattaaaca cagccatgaa agaaggtatg gtagaacaac
      661 atatagcaga taaagtaata gaaataatga tacatttaaa taaatatgca gcaatgagtt
      721 ttgataaatt tgatgtaaat tcagttacag atataactgg ttttggtcta cttggtcata
      781 ctttagaaat ggccaaagct tccgaagtaa gtattgaaat tgaatcaaaa catgtgccaa
      841 ttattgaagg tgcaatagaa atggcacaaa tgggaattat acctgctggt atgtataaaa
      901 atatgcatta tgtatctaaa gatgtagaga ttgtaggaaa tgtagaagtt gcagttcaag
      961 atattttata cgacccacaa acatcaggag ggctgttaat ttcagtaaaa gaagaacttg
     1021 cagaggagct tgtaaaagat atgaaattaa atggagcaat agaagctaaa attataggtt
     1081 ctgtagttcc aaaaggggaa aaatatatta cagttttata aaatattata gagaaagagt
     1141 gattattttg agcaaaagag aattgtttgc aatgttgcct tcagttgatg aagtattaag
     1201 tgataataga attgtagaaa taataaatga atatcctaga agtttagttt tagaatctgt
     1261 gagagaagta atagatttaa aaagacaatt tatattaaga ttaaaagaag atatatcaaa
     1321 ttctgtaaca atagagtttg gtgaaataat agaatctgca atagagagag tgaagttaaa
     1381 ttactcatta tctttaaaaa aagttataaa tgcaactggt acagttattc atacaaattt
     1441 agggaggtct ttacttagtg aagatataaa agatgaatta tggtgtgctg cttcaaggta
     1501 ttcaaactta gaatacgatt tagataatgg agaaagaggt tcaaggtact ctcatttaac
     1561 tagtacaata aaaagactta caggagcaga agatgtacta gttgtaaata ataatgcagc
     1621 agcagtactt cttgtactta gcacaatggc taaaggagga gaagcaattg tatctcgtgg
     1681 agaattagtt gaagtaggtg gctcatttag gattccaagt ataatggctc ttagtggtgc
     1741 agaattagtt gaagttggtt caactaataa aactcacctg aaagattaca aagaggccat
     1801 tacagaagat acaaatgtgc tgatgaaggt tcatacaagt aactatagaa tcatgggatt
     1861 cacagaaagt gtttctatag aagagttagt taatttaggt aaaaaatata aattgcctgt
     1921 aatagaagac ttaggaagtg gagtatttat agatttatca aaatatggtt tatcttatga
     1981 accaactgta ttagattcta ttcgacaagg tgcagatgtg gtaactttta gtggagataa
     2041 gatgcttgga ggtccacaag caggtattat agttggaaaa aaagaatata tagaaaaaat
     2101 gaagaaaaat caacttacta gagcccttag agtcgataaa cttacaatat gtgctcttga
     2161 agctacattg agaatgtatt tagatgagac aaaagctata gaaaatatac ctactttaaa
     2221 aatgttgacg tataaaattg aagagttaga agtaaaagca aataaattat ttgaaaaaat
     2281 aactgcttta aacttaaatg caaacataaa tatagaagat ggattttcac aagtgggagg
     2341 agggtctatg cctcttgaaa ctataagtac aaaggtaata gcaataactc ctgaacatat
     2401 gaatgtttca tctttagaaa agaaactaag acttagtgaa gctcatataa tagccagagt
     2461 gtatgataat aaatatgtac tagatgtaag aactatattt gatgatgaat ttgatgtaat
     2521 agttgatgaa ttgagaaaag catttaatta aataaatttg agttaataaa tattaaattt
     2581 agaagtataa gtatgaagtt ttgattaaaa aatagctaca cacacttgtg actttagtca
     2641 taggaggttt agagggggca aaaaatgaaa aatgtagtaa ttggaacagc aggtcatatt
     2701 gaccatggaa aaactactct tataaaagca ttgactggaa gagagactga tacattagat
     2761 gaagaaaaaa agagaggtat ttcaataaat ttaggattta cttattttga tttaccaaac
     2821 aatagaagag ctggaattgt tgatgtacca ggacacgaaa aatttataaa aaacatgtta
     2881 gctggagcat caggaattga tatagtttta cttgttgtag cagcagatga aggggttatg
     2941 ccacagacag ttgaacattt agatatttta agttttttaa atatcaaaaa tggaatagta
     3001 gttctaacta aatctgatac tgtagatgat gaatttagag agctagtaaa agaagatatt
     3061 tgtgaaaaaa taaaaggaac ttttcttgaa gatgctgaaa taattgaagt tgattctata
     3121 tccaaaagag gtatagacac ccttattgaa aaaatagata aaatgagtga tgatatagaa
     3181 gataaaaatg aaagctctcc agcaagactt aatatagaca gagtattttc aataaaagga
     3241 tttgggactg taataacagg tactttaata gaaggaaaga taagtataga agatgatttg
     3301 gttatatatc cacaggtatt aaaaacaaaa ataagaagca tacaagttca tggagaaaat
     3361 aaagatacag cttatgcagg tcaaagaaca gcaatcaata tatcaaatgt aaaagtggat
     3421 gatataaaaa gaggggatgt tttagctgca ccaaattcat tagaggaatc aatgatgtta
     3481 gatgtaaaat tatcattggt aaatcattta gataaaggat taaaacactg ggatagatta
     3541 agattgtacc atggaaccag agagatactt tgtagagcag ttccactaga taaagaatta
     3601 attgaaaatg gagaaagtgg ctatgttcag ttgagacttg aagaaagcat agtatctaag
     3661 aaaggcgata catttgtagt gagacgttat tctccaatgg aaactatagg cggaggtgta
     3721 ataattgacc catctcctaa aaaacataag aaatttgatg aaaaagttat agaagcatta
     3781 aaaataaaag aaaaaggtga actaaaagat ataatagaag agtacttaaa aaggaatcta
     3841 aaaaattatc caaatataaa agaaataatg agctatagtg gtgctcatga agaagatgta
     3901 aaaaaagcgc ttgaaacact tatatcagga gataaagtat ttattatagg aaatatgtat
     3961 atgcatgtaa atcaatacaa taaattaaaa gaaaatacaa taaagctttt aagtgagtac
     4021 cataagaaat atagacttag aaaagggatg ttaaaagaag aagtaaggtc taagatagag
     4081 agtaatttta agactaggga gatggatatt ttattagaaa aattatctac agaaaatgct
     4141 ataaaaatag aaaataatat agtatcttta ttagattttg aggttatttt aaatgagaag
     4201 caaaaagaaa tagcaaaaaa aatagaaaaa agattaaaat catgtggagt ttcttctata
     4261 ctgacaatag atgaagtttc tgaaggaaat cataactatg ctgaagtatt ggagtcaatg
     4321 ataggtaata aagtagagaa attagatgat ttgtatataa tggacaaaga tatttatgaa
     4381 aatgcaaaga atactttgat aaattatata aaagaaaata aagaaataac attaggagaa
     4441 tacagagatt tgattgattc tagcagaaaa aattgcatga ttatattgga aaacttcgat
     4501 agaaacaaaa taactaagag agtagaaaac aaaagaattt tgttttagca gaaaatgtct
     4561 gtttttgctt taaggtataa agaaggaatt ataatgtcga gtatcgtata attaattagt
     4621 ataaaatatt aattaagagg aagtgtaaag tattgaagag cataatacct gatgtaaaac
     4681 cagatataaa agaaagagaa gacatgagcc ttttatttat aataatgggc tcagtacttt
     4741 tattatttat tataaaagat acagataaat atgaaacttt attacatacc atatatacta
     4801 tattcttagt agctacagga gtaattgtat ttaatactac aaaatttaaa gtagattcat
     4861 tttctacatt tttaggatta atttttatag ctactggagc attggagtgt atatatttat
     4921 tcaattcatt agaaattaaa actcagtttg tgagagaact taacattaca atatcagcaa
     4981 taacagattt atttcctata ttgggagtat acttaagttt caaatttgta aaagataata
     5041 aacaaattta ttcaagtata attttattta tagtaactgt tacattgaca ataatatctt
     5101 tattcataat atctggaatt tgtgaatatt taggaaatgg tacttcacaa tatgctttag
     5161 aagttataat atctatattt ataataatta tttctattgt tagtggaatt gaattaaact
     5221 taagttcaag tgaaggtaaa tgggaatata atgaaaaaaa gatttttaac agaataatag
     5281 taataataat cttatcaaga gttccaaatc tattacatat agttattgat gataggaata
     5341 tagagagaat attatcacat ataataataa atgtagctat atattattta tataattaca
     5401 tagtatctaa gaatattaag aaaacagcat tagaattaca tgacataaat gaagagttaa
     5461 cactaaaaac ggaaagtttg aaagaaaaaa acaaaaagtt aatttatgag acagaaaaga
     5521 tagaggaact taagggaata cttataaaaa gagaatctag actgcaatct acattagatg
     5581 tagcagtaaa tagtatagtt gtatttagta aagatggaga aattacatat gcaaataaaa
     5641 gctttagaaa tacttttgga gactataaag aacaagataa attgactgat aaaataaaaa
     5701 acttcaatga tttgatagaa agcattaatt atgtgtttgt ctatgagaag aatgtagaaa
     5761 aacttattag tacaagcgat aataaagtct atcaagctat atttgcacct ttaattattg
     5821 cttcacaaac agaaggagta ttgtgtgttc tggaagataa gactaagaaa aaagagtatg
     5881 aacgcaaact tattgaagca aataagagaa gtagagagtt tttagaaagt attggagatg
     5941 gcatagtagt gcttgaaggt aataataaaa tatatgttaa taatgcatgt agggaaattt
     6001 ttaaaaataa tttagaaagt attgattttt cattagtatg taaaagtgaa gaaagtatag
     6061 aaaaaagata tgtaatagat ggtgaagtaa agtatgtaga gatgagtttt tctcaatata
     6121 ctaatgctgg tactgataaa actattattg taatacgtga tacaacctct cgaaaaatat
     6181 cgcaaataaa acttgaggaa agtcaatcat catattcaag atttatagat atattacctg
     6241 atggcatatg cttattaaaa gaagatttaa gtataaacta tgcaaataag tcgtttttag
     6301 atatgctagc ttttacaaac atagatgata taaaagattc taatattaag ctattaatga
     6361 atgcgaatac tgaagaaaaa atgaaattta ctgataatat ggctaaagtt ttaaatgaaa
     6421 atagaagtat acttttacta gagtatgaat tgataaatag ttataatgat atagtagaag
     6481 tagaattaag tgcattacct tttgctatat acaatactag atatataatg cttattataa
     6541 aagacttagt acataagaaa tattccgagc aagcggaaaa agaactactt gaaagattta
     6601 aaacagataa aatcaaaacg gagttttttg caaatatgtc tcacgagtta aaaactccac
     6661 taaatgttat atctagttcg aatcaactag tagattcttt ctatagaagt gaaaagataa
     6721 aagattacaa taataatatt aaatctcatg tggatttagt tagacaaagt tcttataggc
     6781 ttcaaagatt aataaataat attatagatt taacaaagat ggagtctgga ttctatacgt
     6841 taaaacttgc taaatacaat attgtatctg taatagaaga cttatttatg aatatagaag
     6901 agtacgcact tagaaaagat ataaaaattc tttttgatac agaacttgaa gaaattaata
     6961 tttatataga caaggttgaa attgagagaa ttatgttgaa tctattatca aactgtatta
     7021 agtttactga taatggaggc tggatatatg taagtattca ttataaaata gataaagtaa
     7081 tcataagtgt aaaagatact ggagttggaa taccacaaga taaattagaa ctgatttttg
     7141 aagaattttc acaagttgat aagactttat ctagaaatac tgaaggaagt ggaatagggc
     7201 ttgctatagt taagaatcta gtaagtcttc atggtggaga tatagaggta ataagtgaag
     7261 tgaacaaagg cacagagttc ttaatttcac ttccaatgaa gggatttagt agtgaacatt
     7321 acacagaaga taagagaata tataatattc aagaaaaaat aaaaatagaa ttttctgata
     7381 tatactatta gaagtgatta tgagtaaaaa taaaataaaa atactatcta tttaaatatt
     7441 taagctataa gaaataggct atcataagat aaattattat gatagcttat ttttagttag
     7501 gctttatatc tatatgtgag aacatataat ctctaaatta ccttcaaaac caaattctcc
     7561 aaagttgttt ggtagtataa aatgcatacc cttttttaaa ttgtattcta cagcattgtg
     7621 tataagtttt ccatttccat ccaatacact gtataaagaa aaaggtgtct tgtgtgtaaa
     7681 agtacacttt ccaaaaatat ctaatttata aacgctaaaa aattcatttg atacaaatgt
     7741 tgtgcatcta aagtctgatg ttgacacaat tttatagtca gtatcaaaat ttatatgagg
     7801 aacattggta acatctattg atttttgaac gtgaagttca cgtttattac ctaaattgtc
     7861 agttctatca taatcataaa ctctataagt agtatctgaa ttttgttgag tttctaaaat
     7921 taatgttcct ttacatatag catgtatagt accacttgga acatagaaaa agtctccctt
     7981 ttttatatta acctttctaa gtagtttgtc ccattcattg ttatttacca tatccacaaa
     8041 ttctttatga gattttgcat tatgacctat tataatttct gcatcatcag aacaatctat
     8101 tacataccaa cactctgtct tacctaactc tccattttca ttttttttag catattcatc
     8161 atttggatga acttgaactg ataaattatc atttgcatcc aatatttttg taagtaaagg
     8221 aaatttatct ccaggtgtgt ttccaaataa ttctctattt ttatcccaca attcagatag
     8281 ttttttacct ttatagattc cattttctat taagcaatct ccatttttat gagaacttat
     8341 agcccaacat tcacctgttg ttggcgaatc tatttcataa ttaaatttat ctttaagtgc
     8401 agtaccaccc catattctat ccataaaaat aggttttaaa aataatggtt ccataatcct
     8461 gttcctccta tgatattaaa tttatcctac aaataattat atataaatac attataaaat
     8521 caatagtaaa aaagttttgt cccttgaaat gacataaagt ataaggagtt attaaataag
     8581 ataaactatg atatataata taaagttcta aattggagtg ataattatgg aattgtttaa
     8641 ggaagaaatc aaaaaatacg aaaaagaatt tttgaaatta ttggaagaat gggtgagcat
     8701 accttcattt tatgatagaa aaactgtatc taaagatatg ccttttggaa aaggggtata
     8761 tgatgcatta aactggtttg aaaatctagg tagagaaaac aatttcaaag tgaaaaatat
     8821 agataatcat gctgttcaaa ttgaatatgg aaatggcaaa gagtatgtag atatatttgg
     8881 acattgtgat gttgtaaatc caggtgaagg atgggatagt gaacctttta aattaaatat
     8941 tataggtgat aaattagttg ctagaggtgt atctgataat aaaggaccta tgatagtgaa
     9001 ttttttagca ctaaaaatga taaaggattt agatattaat ttaaaaagga aggttagatt
     9061 aatagctgga ggaaatgaag agagtggatt taagtgtata aagcattatt atagcaaaga
     9121 gccatatggg gtatgtggat ttactccaga tgctaaattt cctgtattaa atggggaaaa
     9181 aggtggagca ataataaagt tgctttcaaa tatagacgat aaatctttat acataagtgg
     9241 tggaatagag tttaatacaa taccagataa agtttatata aagaatatag aaaaattggg
     9301 taaagataat atatgttttg atattaacaa gatatcgata agttatgata atgacaatta
     9361 tattgtgcat ggaaaaggtg gacattcatc taaacccgaa aaatcaataa atcctatatt
     9421 agctacaatt aaacttttgt cagaaaatat tgatgaaaaa tggacaaaag atttgtataa
     9481 gttgataaat caagataata taaatggaaa tttatttggt ttaaatattg aaggaaaatg
     9541 tgggatttta tcaatggtgc caacaataat aaatatagta gacggaaaat tagaagtagt
     9601 tttaagcgct agatatccag aaatattaag cattgaagag ataatgaaaa aatttaattt
     9661 gtatatggag aaaaataata ttaataaatt tgagcttatt ggggaaaatt taaagcaagc
     9721 aaattatata gataaaaatt caaaactcgt aaaaagtttg catgatatat atataaagta
     9781 ttctggagat ttaaaaaatg atgttagagt aactagtgca ggaagttatg cttctgaaat
     9841 gaataattca gtaatatttg gttgtgaatt tcctgatggt agctttggta atgtccatgg
     9901 tgcgaatgaa tttgctagct tagatagatt tataactgca ataggtatat atgcagaggc
     9961 aataattaca ttatgcaaca aaatataaat acaaaatgtc ccttatatag acatattata
    10021 tatacaatta aaattttact tgatatataa ttgaaatcaa agagaaggtg atacaatgat
    10081 aactaagaaa catgcaataa tagtaaagag tttaagcaat aaagatggat atatgacatc
    10141 aaatgaattg gctatcaaat tggatgtgtc tactaaaact attaaaagat atatagcaga
    10201 tttaaatagt gttttaagca agtatgattt ggtaatagat tcttctagag gtataggata
    10261 taagttaagc ggttctaaaa acaatatagc cagagctatc aaagaagcaa ataaatatat
    10321 tgatggtttt ttagatgatt ctgaagagtc aagaatgtca aatataatat gcatgcttat
    10381 taatagaaat tatatgagta tagaagcaat ggcagaagaa ttaaatctaa gtatagccgc
    10441 tataaataaa ttgtctagta agcttaaaag aaaacttgaa aagtatgact tagttataaa
    10501 atctaaacct tactatggaa gctatataca tggtgaagaa attaatataa ggcaattaat
    10561 tactgactat gcaataaaat tagatgaaaa aagtaaagtg aaggtgtttt tagatgacat
    10621 tagtgaaaac gatatatact gtatagaaag tattttagaa aaacacttaa gagaaaaaga
    10681 tacaataatt tcagataaag attttaatct tttgctttca aaaataatcg tttctgtatt
    10741 taggagtaag agaggtcata gtaacaatat aaacttaatg gatacaagtt atagatttca
    10801 taactactac tttatagaaa gtctaatgaa agaaatatct gataaaattg gatttaaact
    10861 tattgaagat gaagtaattt atatttcaaa ttattctggt gtaatagcct gtaaaggtac
    10921 acaaggaata aaaaacacaa gtgagcttga ggagcgaata aatagtgtta tgagcagtgc
    10981 tcttcaagat atattcctta tttcgggcag tgattataca aaagatgatg agtttatggt
    11041 tgcaatatct gaccatataa aaagattttt aaatagggct agagctaatg taaaatcaaa
    11101 taatccttta cttcatcaaa taaaagagaa gtttcctata gcttttaatt tagccgtgtt
    11161 tttatcaaat aaactagaaa ctgagtttaa tttaaaactt gatgaagatg agcttggata
    11221 tatagctatt cactttgctg cttcaaatga aagaatgaaa aaaaatacta gtaagaaaat
    11281 ttgtatagtt tgtcattatg gtataggaac aggacaactt atttctgaaa agctaaaaca
    11341 gaatataagt gatttaagtg ttgtaggtgt ttatccagtt agatatctcg atatggctat
    11401 aagtcaagat gtcgatttaa tagtatctac tgtcgaatta aaaggatatg aaaaacctgt
    11461 cttatatata gaaaatatat ttgatgatag cttaattgaa aacgtaaaca aagcttttta
    11521 tgaaaaggaa gaaagaagaa agataataag taacatgttt gatgaaaaag cttttttcaa
    11581 tataaaggct aatactaaag aagaggtcat actttcactt tcaaataaac taaaagaaag
    11641 agactttata gaggaaagtt ctataaaaag cataatagat agagaaaata tttcatcaac
    11701 tgaaatagga aatctagtag caatacctca cacaatagtg aagggagata aaaaatctat
    11761 aataggagtt ggaatacttg aaaatccaat aatttgggat aagcaagagg ttcagctagt
    11821 atttatggta ttttttaata ctaaagaaaa gcataatttc tcgatattta aataccttta
    11881 taattttata aaagatgaag gaggagtaag agggattatt aaaatatgtg actttaataa
    11941 attaatggca ctgataggta attaaggagg aaattaataa tgggaacaaa catatttaac
    12001 aaagaatatg tatttttaaa tgttgatgct aagtcaaagg ttgaagtttt aaaatttata
    12061 tcaaaaaaag ctaaagaatt aaatttggca gaagatgaaa gcttagtata tgaaggttta
    12121 atgtctagag aagaacaatt tactacaaat ttaggagaat ctatagcaat tcctcatact
    12181 aaaaatgatg caattgaaaa tccagcagta gtagtgttga agtttaatga agatgttgtt
    12241 tggaatgaag gtgaggataa ggttaaatta gcaataagct tacttatgcc aggaaaaagt
    12301 aaagaaaata ttcatcttaa attgttatca tctctttcta gaaaattaat aaataaagac
    12361 tttaaagatt ctcttttaaa gagtgataat gttgaagaaa tatctaactt aataaatgaa
    12421 gctttaggtt tataattaaa cactaaggtt ataaaataaa aaataattaa ctaaaaatag
    12481 gaggaattta aaatgaataa aaaattagta gcattatgtg cttgcccaat gggattagct
    12541 cacactttca tggcagcaga agcaatagag caagcagcaa aggctttagg ttatgaagca
    12601 aaagttgaaa ctcaaggtgc agatggtgta caaaatgaat taacaagaga tgatatatta
    12661 ggagcaacta tgataataca tgcagtagca ataactccag aaggtatgga aagatttgat
    12721 gggtgtgaag tttacgaggt tgagttacaa gaagctataa aaaatgcaga gggtgtcata
    12781 aaagaaatag aagaagattt aggaatataa taaatttatt gtggaggaaa tagaatggca
    12841 attaagaaaa aagttataaa cagttcaggt gcttctacaa caggaggaaa taatgtagga
    12901 aaatctactc catctagtcc aactcaatca aagggaaatg gaaaatggaa agaaataagt
    12961 aaacatataa tgacaggtat atcatatatg ataccagttt tagtaatggg tggacttata
    13021 ggagctttat ctcaactaat accatatgct atattaggac ttgacccatc tgtaggtata
    13081 gttgatgcta tgaactcagg agaatttaca gggttcaagc ttagtctttt aaatatagca
    13141 caattaatgt caaatttcgg atttactttg tttggatttg caataccact atttgcagca
    13201 ttttgtgcaa actctatagg tggaaaaaca gccttaattg caggatttat aggtggatat
    13261 atagctaata aacctgtagg tgtaccacaa tttgttgatg gacaatggac tgaagttgtt
    13321 ccagttgcat caggattttt aggcgcaata ttaatagcat tcataatagg atattttgta
    13381 aaatggttaa ataaatctat aaaagtttct cataactggt tagcttttaa aacaacattt
    13441 ttaataccac taatagcgtc attagcttgt atggtattga tgatatttat aataactcca
    13501 tttggtggac taattaatga aagtatgaaa aacttcttaa cagcagcagg agcagcagga
    13561 gaatatgtat atgcaacagc tttggcagca gctacagcat ttgacttagg aggaccaata
    13621 aataaagcag caggattcgt tgcacttggt ttaactacag agaatgtact tccaataaca
    13681 gcaagaacaa tagctatagt tgttcctcca attggattag gattaacaac attattagat
    13741 aaaaaactag taggaagaag agtatatgat agacaattct atcaagcagg aaaaacttct
    13801 atattcttat catttatggg tatatctgaa ggagcaatac catttgcact tgaaagacca
    13861 ggctttgtta tacctttaaa catagtagga agtgtaatag gagctattac aggtataatt
    13921 ctaggagcaa ttcaatggtt cccagagtca gcagtatggg catggcctct agtagacaac
    13981 ttatttggat atataatagg tatagcagtt ggtgctatat ttatagcagt aggaaatata
    14041 ttctatagaa ataaattaat aaaagatggt aaacttgttg tagattatat cgactaatat
    14101 ttaattttta ctatctataa ataaccttgg ctggtaatat ggttttaatc aatattacca
    14161 gccttaccta tatgattaat tagaggcggt ggagataaga tatggataag atagaaaaag
    14221 taagagagta ttttagagaa tatgatatag atggatttct tatcaatagt cctacaaata
    14281 agttttatgt tggaaattta tttagttctt caggttatgt ttttataacg aaagaagcac
    14341 aatatataat agtagatttt agatattttg aagaaattaa aagaaaatcg agcttgttta
    14401 atgtagtatt aatggacaaa actagaactc attttgatat aattaatgat atttgtagag
    14461 aacagaatat aaaggaaatt ggatttgagg gaaatgaagt atcttttgat ttatatagaa
    14521 gtatgagtaa taagttaagt gcaaccttaa aatcagttga tttatctaca ttaagggaaa
    14581 caaaaaatga agacgaaata aaatatataa aaaaagcttg tgaaatagtt gatgctacat
    14641 tttatcatat aatagacttt ataaaggttg gtatgactga aaagcaagtt gaaaatgaaa
    14701 ttgttagagt tataaaagat ttaggtggtc aaaaagaatc tttcgataca atagttgcat
    14761 ctgggcttag aggagcattg cctcatggaa aagcaagtga aaaagttata gagtatggag
    14821 attttgttac ttttgacttt ggtgctaagt acaataatta ttgttcagat attactagaa
    14881 ccatttgtat gggtactata aataaagagt tagaagaaat atataatata gtaagaaaag
    14941 ctaatgaaga gtgtataagg gttttaagac caggaatgac tactggagag atagataaag
    15001 ttgcaagaga tataattggt tcatatggat atgccaataa ttttggtcat aatctgggtc
    15061 atggtgtagg aattatggtt catgaatatc ctgcattagc tcctgaatca aatgaagtat
    15121 taaaagaagg aatgattgta actatagagc ctggtatata tgttccaagt cttggaggcg
    15181 taagaataga agatgatgta cttataacaa aagatggatg tatgagattg actacatcta
    15241 ctaaggattt aatagttgtc agttaaaaat aaattaggta aatattttat aacaataaga
    15301 aaagaagagg taaatttaaa tgtataattt tgataataaa ccagatagag taagtgagaa
    15361 gtgtagaaaa tgggatttaa atattataag agataaattt ggagatatta gagaagattt
    15421 tgtaccaatg tggattgctg atatggattt taaaatacca acagaaatag aaaacaaatt
    15481 tattgaagct gtaagaagag gtgtatttgg atatacatac tgttatgatg aattttatga
    15541 ttcagttatt gggtggcaaa aaaatatgca tgaggtagat gtagaaaagg aatggattac
    15601 attaacttat ggaactgtat cgacacttca ttatgtagtg caagcttttt gtaaaaaagg
    15661 cgatagcata atattaaaca caccagttta tgaccctttt gaatcatcag caaaaaaaca
    15721 gggtgtaaat gtaatttgta atacattaga tgttgtaaat gatagatatt atataaattt
    15781 tgataaatta gaagcacaaa ttaaggaaaa taagcctaaa ttaatgatgt tttgtacacc
    15841 acataatcca tctggaagga tatggacgat tgaagaaatg acaaaagtag caactatatg
    15901 taaagaaaat aatgttattt tggttgcaga tgaagtacat gcagaacata ttcattatgg
    15961 taaatttaat tcaatactta agattgaaaa agagctttta gaaaatgtta ttctattgac
    16021 atcaccaaat aaaggattta atcttggtgg attaaagacc tcttattcaa tagttataaa
    16081 taagaatata agagacaaat ttagaaataa actaaagcaa aattctataa cttctccaaa
    16141 tgtgtttgga ataatagggt tgataactgc ttataatgaa tgtcatgagt ggttaaatgg
    16201 tgtaaatgaa tatataaagt caaattatga acttttagaa acttgggtaa ataaatatag
    16261 taagataaaa ttgatgaaaa tggaatcatc ttacttagct tggatggata taagtggatt
    16321 aggcataagt gcaagtgagt ttacagatag gttggctata gatacaggag tattattaga
    16381 agatggaagt cattttgtac aagatggtga taagtatgtt agaataaatt taggaactca
    16441 aaaagaaaat gtaattgaag ctttaaatag aatggatttg tttttaaaat ctttataaaa
    16501 atttggttaa attatttatt gaaaatataa aa
//
LOCUS       DAFNNB010000082        16469 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0083, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000082 DAFNNB010000000
VERSION     DAFNNB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16469
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0083"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(65..433)
                     /locus_tag="L3M60_002214"
     CDS             complement(65..433)
                     /locus_tag="L3M60_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBN6226874.1"
                     /translation="MTRPSIYNSQGYTCAEALIKSYNEEHNTDIPISIGSGMGFGMNV
                     GSVCGAVNAAVVIIGYLNGRNSNLDENIARKYSRELMNRVRNKYSSEICVSLKKNKVT
                     CEEIINFTYDALNDMLKNQK"
     gene            862..1818
                     /locus_tag="L3M60_002215"
     CDS             862..1818
                     /locus_tag="L3M60_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBN6226875.1"
                     /translation="MKKILITGALGQIGSELTIKLRNEYGEQNVIASSRRAKEGNPVC
                     ESGIFEILDVTDENRFFEIAKKYDVDTIIHLASLLSAVAESKPLEAWNLNMNGLINGL
                     EVARELDCKFFTPSSIAAFGENSPKNMTPQDTLQRPNTMYGVTKVSGELLCDYYHSKF
                     GVDTRGVRFPGLISYVTPPGGGTTDYAVDIYYEALKNKRYKSYIAEGTKMDMMYMPDA
                     LQSIVDLIEAPADKLIHRNAFNITAMSFSPEEIADSIKKYIPDFVIEYDVDPVRQSIA
                     DSWPNSLDSSSAVKEWNFKFSYDLDKMTKDMLEKLSEKGIGK"
     gene            2037..2384
                     /locus_tag="L3M60_002216"
     CDS             2037..2384
                     /locus_tag="L3M60_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226876.1"
                     /translation="MLNLKEIREEIIKKYIGDILISELTDELKSIIITDLQSNPFKSN
                     IELSIIPANIEIFKKIENILLDSKVNLKMGWERILINYILKDLFELNYEINKSCNEPL
                     MFRIIISIDSYDD"
     gene            2424..3398
                     /locus_tag="L3M60_002217"
     CDS             2424..3398
                     /locus_tag="L3M60_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBN6226877.1"
                     /translation="MKYKLFIDFEFNILDDNKNKPKEYNGTELISIGGVLVDNEFNTI
                     DNYYSLVKPRYNKILSNKCKNLTKLNQLDINNAPNLLYVMDDFYKWFCKFDDVTIYNW
                     GDFDIIGLLTSFRVYKYTGKCLELFNMMIDIQPFISQHITYNNRILSKQISLLNMKKI
                     FSVEGDIKHNSLSDAMDLMNVCKCFFFNYPKNIDILEELYNKLPAFKTPLYYIPYFED
                     ENFELKFDRTPEDIIVYLKQILNILNISKKEIYFKKRSVLVNNSKIINFKNLSSSFKL
                     IKINEFEEYPDFILKLGDKKSFVESVIKINKSNRKSIKNLILKLSKKP"
     gene            3504..4163
                     /locus_tag="L3M60_002218"
     CDS             3504..4163
                     /locus_tag="L3M60_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBN6226878.1"
                     /translation="MTVVLIRSIILYITVLIALRVMGKGEIAEMNCFDLVITLLIAEV
                     ASVPMENNNIPIINGVAAISGLVIMQTLISFLSLKSRKLSSFLSGKPSVLIDKGKIVY
                     KELKKERVSIDELLEQLRIQGYFNLKDVQYAILETDGNLSVVPASSYNSTPPRAFNHL
                     PIPLILDGRIINKNLDIAQKDTNWLMGILKSNHIETFKDVLICVLDENDKIFIQNKKG
                     D"
     gene            4166..4546
                     /locus_tag="L3M60_002219"
     CDS             4166..4546
                     /locus_tag="L3M60_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4363 family protein"
                     /protein_id="HBN6226879.1"
                     /translation="MKSTTFVILWTILFVLFGFYVNNKLYDFTEGYKNNISVLEKSIE
                     NENWEKAQKEADSISTSWNKEKNHWYKVLNHEYFDEIGLRFNILDKAIYTENKLKSLE
                     EIESIKTYLGNIIESVKFDVNYIF"
     gene            complement(4635..5798)
                     /locus_tag="L3M60_002220"
     CDS             complement(4635..5798)
                     /locus_tag="L3M60_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6226880.1"
                     /translation="MRTVKQVSDLTGISVRALHYYDEIGLLKPNKITDAGYRLYDDDS
                     IKTLQQILFFKEIDIPLREVKEIMSSPYFDKLEALKNQRKLLILKRKRLDELIELINQ
                     TLRGEGNINFKEFDMSEYFDVLEEFKREHKSKIIKIYGSVEKYNEYIERVKFNEDKIA
                     KMAIKQYGTIEKFAQAIKTNFSSDILNLGEKFDRYKNDCLKEQHPKLKELYRKLAEDL
                     SKNPSSTELQEIAKEITDISKKDYEIFSMDTGDDNWYYMVQNYLVNPMWIEEVDKKYG
                     SGAGKFIGQVLKTYLRDRKPKINTLYEKLVEDLSRDCSSKEIQSIVEEIDNEMKRSND
                     FYKIDNGERYFDYMSELYLQDSNYIKVTDKNYGNGASKFIGEAFKIYFDNNNC"
     gene            complement(5904..6950)
                     /locus_tag="L3M60_002221"
     CDS             complement(5904..6950)
                     /locus_tag="L3M60_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226881.1"
                     /translation="MRKIIEIKHHSHDYECMWNGIEDLYINKTGECLPNNFFFLLSGF
                     GSFCYMKTNKSELKRMVALGDGRTKKMYKFLAPIVGFEYKHHEFKKYEQALKKAKSEI
                     DSGHPVVLGALDMYYLPYYKKLYHIEHLPFHYVLMVGYDDEKVYLYDCGRTELLNLSY
                     DKLRESMNCSYPGLSKENTICTIRMNTSKNKYKIAKEVIAIKRDMFLNPPKGFLGYKG
                     FEKFIKELSQWKTELEKDDYDKILTNMIMFFGTVPTIPNAIKGIKEPDTIQFKGGFDK
                     MSKMLKSIGEEYKNDSFIQVAEIFDKTSLIIEKISNIIVDYLTQKYDDTDQLPLLFSE
                     VLKDMKTGYLILNV"
     gene            complement(7200..8399)
                     /locus_tag="L3M60_002222"
     CDS             complement(7200..8399)
                     /locus_tag="L3M60_002222"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBN6226882.1"
                     /translation="MKEKVVLAYSGGLDTSIIIPWLKENYEDIDVIAVCGNVGQEDKM
                     EDVYEKALQSGASKAYVDDISEEFVTETIFKAVKAEAKYEGKYLLGTSLARPIIAKKL
                     VEVAHKEGAKYICHGCTGKGNDQVRFEATIAALDPTIKVIAPWRIWDIKSREDAIDYA
                     EKHNIKVTATKAKIYSVDANLWHVSTEGGDIEHLENEHKKDVYKQCVDPEDACDVAEY
                     VEIYFEKGVPKKVNGEELSPVALIHKLNELGCKHGIGVIDIVENRLVGMKSRGIYETP
                     GGTILYETHNILESATLDKDTLHFKQMVSYKYGELIYNGLWYCKLRESIDAFMEQTQD
                     NVTGTVKVKLYKGNIKPAGIFTENALYDEGISSFGNSELYDHKDAEGFINLFTLPLKI
                     RAMKAGK"
     misc_binding    complement(8652..8859)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8970..9707)
                     /gene="truA"
                     /locus_tag="L3M60_002223"
     CDS             complement(8970..9707)
                     /gene="truA"
                     /locus_tag="L3M60_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBN6226883.1"
                     /translation="MRNIKMIVAYDGSRYKGYQKLGDNNMTIQEKLENVLSKMTNETV
                     EIIGSGRTDMGAHARGQVVNFRTNCMDSLDKIQKYLYEYLPEDIVVKAVEEVDERFHS
                     RYNVKSKTYMYRIDNNKYHNPFIRKYATHVSKKLDLDRMRKASEYLVGEHDFTSFASS
                     KSKKKSNVREIYSINIKEDDNIIEIYVEGNGFLYNMVRIIVGALIDVGLKRRAPQDIK
                     YMLESRDRCQSSDTAPAKGLCLWKVRY"
     gene            complement(9729..11162)
                     /locus_tag="L3M60_002224"
     CDS             complement(9729..11162)
                     /locus_tag="L3M60_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6226884.1"
                     /translation="MLHFEGLRKKVIKNYFIIIIIMVTLFEGLFMFYIQNYYYDSVKQ
                     LLESEIKYADEYNAITMETTSFEKKVKNIFDKQPLTKNSEFGISIIDKDKNIILDQYG
                     FKSKEKANYEDVNNALKDIKTKNLTPYTYRIPDTGEHVMSISLPLKVNNIIEGVVRYT
                     VSLDAIDNAILKQATWLILAGIFILIIAILISLKFAETLIKPLRELKKFANELAVGNY
                     NIKLEKMKIVDDEIGDLAQTFEHMAHEIDKSEKLKEEFISSVSHELRTPLTSIKGWSE
                     TLGYESITREELDLGLGIIQDETERLIKLVEELLDFSRLSSDRIKLHVDIVDVEGLIV
                     GVVNQLKVKAAEKDISLLFEFENEFIENIQGDKNRLRQVLINLIQNSFKFTSQGGYIK
                     VVAFQDEEITTISVEDNGSGIEKQNLNKVLDKFFQEDYNKAGSGLGLAISNEIVKLHG
                     GRMKIESEKNVGTKITFNIKNKFAKQA"
     gene            complement(11165..11863)
                     /locus_tag="L3M60_002225"
     CDS             complement(11165..11863)
                     /locus_tag="L3M60_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226885.1"
                     /translation="MKEKILILEDEIGIRSFVSINLKREGYEIVEAGTGREAIEKMTT
                     ENDITIALLDVMLPDISGIEVCKFIRENFDQVGIIMLTAKAQEDDKIEGFISGADDYI
                     IKPFSIKELLVRVSALIRRVAKDDSSVKSSEIVSPPFILDIDKRKLFKNGKEIELTPT
                     EFSIVKYLISNAKQSLSRDQILDEVWGTNYLYDFKIVDVNIRRIRNKIEDDPSKPKYI
                     QTIWGYGYCFRKEE"
     gene            complement(11892..13280)
                     /locus_tag="L3M60_002226"
     CDS             complement(11892..13280)
                     /locus_tag="L3M60_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226886.1"
                     /translation="MKVLKLITVVAMMMLFVTGCNIKTESPEQLAKIPNYDDTKKVLY
                     DGIDQLLKPDSSMILPSNTKEVGKINKVDLNSDGIDELVVFEQKEDLSNNVSQVGFVT
                     LSYNGEKYVLGDHFLENGESIEYANFYDLDSDGYKEVILLVKSKDKTNMHIYKVVDNE
                     ITKIYDLDASWLQNKEDFNDMKVKIGYIDGDDKLDILMLHLNNKTNEMFASVANFDGS
                     MKIKDSVKFENVKNLSELYITLGNVASSVGNSSAAIKGIVLDIPILKDNNYITQILYM
                     KDGKINKAFSDYDKTITKSYYIPVDDIDKDKIIEIPIVAGNTGNNRNTYSSKASANIS
                     WYRWNGKEGADSSLIFTSQIYYNYKYNFKLFIPNNLFDKILVEQEFVGEKAVFKFYYF
                     DYSDRKNLFNIVVESKNKLEDRKNTSTQNSIVLQESDEYTFLLVVNNANEMDKLDMTI
                     DALKEYFSLIYG"
     gene            complement(13416..14564)
                     /locus_tag="L3M60_002227"
     CDS             complement(13416..14564)
                     /locus_tag="L3M60_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HBN6226887.1"
                     /translation="MKNYTLISPCFFGMEKMLAREITNLGYEIIKTEDGRITYKTDEF
                     GIAKSNMWLRCAERVHLKIAEFEAKSFDELFENTKRINWSRYIPYGAQFPISKASSIK
                     SKLYSTPDVQAIVKKAIVESLKKSYLEDGLLKEDKEKYPIFVFIHKDKVTISIDTTGD
                     ALHKRGYREKANKAPIRETLAAGLIYLTPWKAGRVLVDPMCGSGTILIEAAMIGINMA
                     PGLNREFISEKWRTLDKKIWWDVRKDAFNKIDNESKFKIYGYDIDEESIDIARENAEI
                     AGVDEYIEFNVGDATQFKSEDEFGFIITNPPYGERLEDKDSVKQLYKELGYAFRKLKN
                     WSYYLITSYEDFEYEFGQKADKKRKLYNGMLKTNFFQYPGPKPPRNNK"
     gene            complement(14619..15437)
                     /gene="larE"
                     /locus_tag="L3M60_002228"
     CDS             complement(14619..15437)
                     /gene="larE"
                     /locus_tag="L3M60_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HBN6226888.1"
                     /translation="MEVNFVKEKEKLDKLKKMLLELGSVVVAYSGGVDSNFLLKVAKD
                     TLGENVVAVTIHAMMHSSREIEEAKQYTQNFGVKHIILNIENFDLKEFKENGIDRCYH
                     CKKYIFSKIKEVAKEHNIKYIVDGTNIDDLGDYRPGLKALSELGVISPLKDSGLKKEE
                     IRSLSKILGLKTFNKPSFACLASRIPYGVEITDENLRIIEKSEEYLSNLGFSQFRVRM
                     HGDIARIEVGQEELGKFFENNNFNKVDTKLKIFGFKYVTLDMSGYKMGSMNLNV"
     gene            complement(15746..16447)
                     /locus_tag="L3M60_002229"
     CDS             complement(15746..16447)
                     /locus_tag="L3M60_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HBN6226889.1"
                     /translation="MSIEDLYDFECVPLNNEVDIDVLFEKMIEDGKEKEYTPVIVIDE
                     KVGLLRKNIDIIIKEYGSLENYREYCLTKYNEIDVNQFFDFRKREISELLKELVDLED
                     DFYDYKHFRPIMNIDIFHEYNKIYIAKVPTIKPYEVFAYIPIGGFNDCPRDEEHIAIA
                     KYWYEKHGIFPIAIGCDTVQYSVKDLIKDGKKFDELCIELVFYCEDIIIQGYETLNAL
                     KDVLKRSTILLFWWD"
BASE COUNT         5552 a         2661 c         1746 g         6510 t
ORIGIN      
        1 cctatttaaa atatcataaa aaaaacaagt attttatcga atgcaaaata cttgtttctt
       61 agaattattt ttgatttttt agcatatcat ttagagcatc atatgtgaaa tttattattt
      121 cttcacaagt tactttattt tttttaagag atacacatat ttctgagctg tatttatttc
      181 ttactctatt cattaattct cttgaatact tccttgctat gttttcatct aaattactat
      241 ttcgtccatt taaataccct attataacaa ctgcagcatt tacagctcca caaacacttc
      301 caacattcat tccaaacccc atgccacttc ctattgagat tggtatatct gtgttgtgtt
      361 cctcattata agactttatt aatgcctctg cacaagtgta tccttggcta ttatatattg
      421 atggtcttgt catttttata cttcctttca aattttattt tatatgtttt actattttaa
      481 atatgctaat ggtctctata ttatatatac tatttttgta tctataaaat ccaacctata
      541 ttcactataa ataaatttat attacttttt atttataaaa ttactttaat ttaaagcttt
      601 acttgatatg cttttattat atattaaatt aattatactt tctgtgactt agtcacataa
      661 tctatatata ataatttaat ctagtctttt attgttatac aatagaccat tatatattaa
      721 cttttaattt ttagaccttt actgcactta attcaattag accatatatt attatcttta
      781 attctttgta tttaaacaaa agatttgcta aaataaaaat aagtagtact acataaatta
      841 aacccttagg aggttataaa attgaaaaaa atacttataa caggtgcatt aggacaaata
      901 ggttcagaat taacaataaa gcttagaaat gagtatggag aacaaaatgt aattgctagt
      961 tcaaggagag caaaagaagg taatccagta tgtgaatctg gtatatttga aatattagat
     1021 gttacagatg agaatcgttt ttttgaaatc gcaaaaaaat atgatgtaga tactataatt
     1081 cacctagctt ctttactttc tgcagttgct gaaagtaaac ctctagaagc ttggaactta
     1141 aatatgaatg gattaataaa tggtcttgaa gtagctagag aattggattg taaatttttt
     1201 acacctagtt caattgctgc ctttggtgaa aattcaccaa aaaatatgac gcctcaagac
     1261 acattacaaa gacctaatac tatgtatggt gtaactaaag tttctggaga attactatgt
     1321 gattattatc attcaaaatt tggagtagac actagaggtg ttagatttcc aggtcttatt
     1381 tcttatgtta cgcctcctgg tggaggaact acagattatg cagttgatat atattatgaa
     1441 gcattaaaaa acaagagata taaatcttac atagcagaag gtacaaaaat ggatatgatg
     1501 tatatgcctg atgcacttca atcaatagtg gatttaatcg aagctccagc ggataaactt
     1561 atccatagaa atgcttttaa tattactgct atgagctttt ctcctgaaga aatagctgat
     1621 tctataaaaa aatatattcc tgattttgta atagagtatg atgtagaccc tgtaagacaa
     1681 agtatagctg attcttggcc taattctcta gacagtagta gtgcagtaaa agaatggaac
     1741 tttaagttta gttatgattt agataaaatg accaaagata tgttagaaaa attatcagaa
     1801 aaaggaatag gtaaatagta acctatcatt tttcatcaaa ataggcaaca tactccacct
     1861 ttaacataag cagggtggag atataagcct ggaatatgtt aatataagtt taaattagat
     1921 aaaaaatgat ataggtgaaa acttttaatc ttaaaatata tccaaataag attcaaaaat
     1981 aaataatatt attaaaaaga aaggaaaagc tactgtttaa gtagcaggta ctaatattgc
     2041 taaatttaaa agaaattaga gaggaaataa taaaaaaata cataggtgat attttaattt
     2101 ctgaacttac agatgaatta aaaagtataa ttataacaga tttacagtct aacccgttta
     2161 aaagtaatat tgagctcagt ataataccag caaatataga aatatttaaa aaaatagaaa
     2221 atatattact agactctaag gttaatttaa aaatgggttg ggaaagaata cttataaatt
     2281 atatcttaaa agatttattt gaattaaatt atgaaataaa taaaagttgc aatgaacctt
     2341 tgatgtttag aatcattata tctattgatt cttatgatga ttaggctcaa taaataattt
     2401 acctgaggat gtgctttaga tttatgaaat acaagttatt tatagacttt gaatttaata
     2461 tactagatga taataaaaat aaaccaaaag aatataatgg cactgagtta atttctattg
     2521 gtggagtttt agttgataat gaatttaata caatagataa ttattactct ttagttaagc
     2581 caagatacaa taaaatactt tcaaataaat gtaaaaactt aaccaaattg aaccaactag
     2641 atatcaataa tgctcctaat cttttatatg ttatggatga tttttataaa tggttttgta
     2701 aatttgatga tgttacaata tataactggg gtgattttga tataattggt cttttaactt
     2761 catttagagt atataaatat acaggtaagt gcttagagtt atttaatatg atgattgata
     2821 ttcaaccttt tatatctcaa catattacat ataataatag aatcttatct aagcaaatat
     2881 ctttactgaa tatgaaaaaa attttttctg tagaaggaga tattaaacat aactcacttt
     2941 cggatgctat ggatttaatg aatgtatgta aatgcttttt ctttaactac cctaaaaata
     3001 tcgatattct tgaagaatta tataataaat tacctgcttt caaaacaccc ttatattata
     3061 tcccctattt tgaagatgaa aattttgaac tcaaatttga tagaactcca gaagatataa
     3121 tagtatatct taaacaaata ttaaatatac taaatataag taaaaaagaa atttatttta
     3181 aaaaaagaag tgtgttagtg aataactcta aaattattaa ctttaaaaat ctatcttctt
     3241 ctttcaaatt aataaaaatc aatgaatttg aagaatatcc tgattttatc ttaaaattag
     3301 gagataaaaa gagttttgtt gaatctgtta ttaaaataaa taaaagtaac agaaaatcta
     3361 taaaaaattt aatattaaaa ttatcaaaaa aaccttaagt agttgaatcc aattatttaa
     3421 ggttttttat actttgaatt aaaattttta tataggacat actaactaag tgtaaatatt
     3481 ttttaaagga gtaattgata gatatgacag tagtattaat aagaagtatt atattataca
     3541 taacagtgct aattgcactt agagtcatgg gaaaaggtga aatagctgaa atgaactgtt
     3601 ttgatttagt tataacacta ttaattgcag aagttgcatc tgtcccaatg gaaaataata
     3661 atataccaat tataaatggt gttgcagcaa tatctggact tgtaattatg cagactctaa
     3721 tttcattctt atcattaaaa agcagaaaat taagttcatt tttatcaggt aaaccttctg
     3781 tgttaataga taagggaaaa attgtatata aagagcttaa aaaagaaaga gttagtatag
     3841 atgaattatt ggagcaactt aggattcagg gatactttaa cctaaaagat gtacagtatg
     3901 ctattttgga gactgatggt aatctaagtg ttgtaccagc atcaagctac aatagtactc
     3961 ctccaagagc ttttaatcat ttacctattc cacttatact ggatggtaga ataataaata
     4021 aaaatctaga catagctcaa aaagatacta actggttaat gggtatttta aaatctaatc
     4081 acatagaaac ttttaaagat gttcttattt gtgttttaga tgaaaatgac aaaatattta
     4141 ttcaaaataa aaagggtgat taatattgaa atctacaaca tttgttatac tatggactat
     4201 cttatttgta ttatttggat tttacgttaa caataaacta tatgatttta cagaaggata
     4261 taaaaacaat atatctgttt tagaaaaatc tatagaaaat gaaaattggg aaaaagctca
     4321 aaaagaagct gattcaatat ctacaagttg gaataaagaa aaaaaccatt ggtacaaagt
     4381 tttaaatcat gagtactttg atgaaatagg attaagattt aatattctgg acaaagcaat
     4441 ttatactgaa aataaattaa aatctttaga agaaattgaa agtataaaaa catatctggg
     4501 aaatatcatt gaaagtgtca agtttgatgt aaattatata ttttaatgac tttaaataag
     4561 gatatagatg gagctaatct atatccttac ttttatgctt ttatgaagct tacacatttt
     4621 ttatttccaa attttcaaca attattatta tcaaaataaa tcttaaaagc ttctccaata
     4681 aacttagatg caccattacc ataattcttg tcagttactt ttatgtagtt agaatcttgt
     4741 aaataaagct ctgacatata atcaaagtat ctttctccat tgtctatttt ataaaagtca
     4801 ttacttcttt tcatttcatt gtctatttct tcaacaatgc tttgaatttc ctttgaagaa
     4861 caatctctac tcaaatcttc tacaagtttt tcatacaatg tatttatctt tggttttcta
     4921 tctcttagat aagtttttag tacttgtcca ataaacttgc ctgctccact tccatacttt
     4981 ttatctactt cttctatcca cataggattt accagataat tttgaaccat ataataccag
     5041 ttgtcatcac cagtatccat agagaaaatt tcataatctt ttttagatat atctgttatc
     5101 tcctttgcta tttcttgaag ttctgttgaa gagggatttt tgcttaaatc ttcagcaagc
     5161 tttctatata attcttttaa ttttggatgt tgctctttta aacaatcatt tttgtatcta
     5221 tcgaattttt ctcctaaatt caatatatca ctgctaaaat ttgtttttat agcttgggca
     5281 aacttctcaa tagttccata ttgttttata gccattttag caattttatc ttcattgaat
     5341 ttaactcttt ctatatattc attgtatttt tctacactac cataaatttt aattatctta
     5401 ctcttatgct ctctcttaaa ttcttctaaa acatcaaaat actcgctcat atcaaactct
     5461 ttaaaattaa tatttccttc tccccttagg gtttgattta taagttctat caattcgtcc
     5521 aatctttttc gttttaaaat aagtaatttt ctttgatttt ttaaagcctc taacttatca
     5581 aaatacgggc ttgacattat ctccttaact tctcttaaag gaatatcaat ctccttgaaa
     5641 aataaaattt gttgaagagt tttaatagaa tcatcatcat aaagcctata tcctgcatct
     5701 gtaattttat ttggttttaa aagacctatt tcatcataat aatgtaatgc tcttacactt
     5761 attccagtta aatcggaaac ttgttttact gttctcatat ctacacctca aatttgattt
     5821 tatagctatc aacctttacg aatctattca agctatattt tcatattaca ctattacgta
     5881 atgttagagt caataatcta tttttataca ttcaatatta aatatcctgt tttcatatcc
     5941 tttaaaacct cagagaatag taatggaagt tggtctgtat catcatattt ctgagtcaaa
     6001 taatctacta taatattgga tattttctcg attataagag aagttttatc aaaaatctca
     6061 gctacttgta taaaagagtc atttttatat tcctctccaa tacttttaag cattttactc
     6121 attttgtcaa atccaccttt aaattgaata gtgtctggtt ctttaattcc tttgattgca
     6181 tttggaattg ttggtactgt tccaaaaaac ataatcatat tggtcaaaat cttatcataa
     6241 tcatctttct ccaattcagt tttccactgt gacaactctt taataaactt ttcaaaacct
     6301 ttatatccca aaaatccttt aggtggattt aaaaacatat cacgctttat agcaataact
     6361 tctttagcaa ttttatattt attttttgaa gtgttcattc ttattgtgca aatagtattt
     6421 tctttgctta atccaggata actgcaattc atactttcac gtaatttatc ataagaaaga
     6481 tttaataatt ctgttctccc acaatcatac aaatatactt tttcatcatc atatcctacc
     6541 attaatacat agtgaaaagg aagatgctct atatgatata actttttata atatggtaaa
     6601 taatacatat ctaaagctcc taaaacaact ggatgtccac tatcaatttc agattttgct
     6661 ttctttaatg cttgctcata ttttttaaac tcatgatgtt tgtattcaaa accgacaatt
     6721 ggtgctaaga atttatacat cttttttgtc cttccatctc ccaaagctac cattcttttt
     6781 aactccgatt tatttgtttt catataacaa aacgaaccaa aacctgatag caaaaagaaa
     6841 aaattgtttg gaagacattc acctgtttta tttatatata aatcttctat accattccac
     6901 atacattcat aatcatgtga atgatgtttg atttcaatta tttttctcat ataatctatc
     6961 tcctcactat aagtttttgc tagattcagt ataaaccctc ttacaatgag agagtcaata
     7021 gataactatt atatttataa aatttatatt ttaggtatta tattttcata ttaataaccg
     7081 atttgatact ataatatatt ttgttatatt gtttactgca taatttaata attccaaata
     7141 aaaaagagca tataaaaacc tagaaatcgt ttttacatac tcttgaataa tctattttat
     7201 tattttccag ctttcatagc tctgattttt aatggtaaag tgaataaatt aataaatcct
     7261 tctgcatctt tgtggtcata aagttcacta tttccaaaag aagatattcc ttcatcataa
     7321 agagcatttt cagtaaatat tccagcaggt tttatgttac ctttgtaaag tttaactttt
     7381 actgttcctg taacattatc ttgagtttgc tccataaatg catctataga ttctcttaat
     7441 ttacaatacc ataatccatt gtaaataagt tctccatatt tataagacac catttgtttg
     7501 aaatgtaatg tatctttgtc taaagtagca ctttctaaaa tattatgtgt ttcataaagg
     7561 attgttcctc ctggagtctc atatatacct cttgatttca taccaacaag tctattctca
     7621 actatatcta taacaccaat tccatgttta caacctaact catttaattt atgaatcagt
     7681 gcaactggag ataattcttc tccatttacc tttttaggta cacctttttc aaaataaatt
     7741 tctacatatt cagccacatc acatgcatct tctgggtcta cacattgttt gtaaacatct
     7801 tttttatgtt cattttctag atgctctata tctccaccct ctgttgacac atgccaaagg
     7861 ttagcgtcta ctgaatatat tttagcttta gttgctgtta cctttatatt atgtttttca
     7921 gcatagtcta tagcatcttc tcttgatttt atatcccaaa ttctccaagg tgcaataact
     7981 tttatagtcg ggtcaagagc tgcaattgta gcttcaaatc taacttggtc attacctttt
     8041 cctgtacatc catggcatat atatttagca ccttctttat gagcaacttc aactaatttt
     8101 ttggctatta taggtcttgc taaagaagtt cctaataaat actttccttc atatttagct
     8161 tctgctttta cagctttaaa tatagtttca gttacaaatt cttcacttat atcatccaca
     8221 tatgcctttg atgctccact ttgtaaagct ttttcataca catcttccat tttatcttcc
     8281 tgacctacat ttccacaaac agctataaca tctatatctt cataattttc ctttaaccaa
     8341 ggtattatga ttgatgtgtc aagtcctcct gaatatgcta atactacttt ttccttcatt
     8401 tttaattcct cccaatctta aattataatt tttattttat agaaattctt aatattgaat
     8461 gactttgaga atttagattc acataaaatc attttaatac taaaaaagcc ccttagtatt
     8521 ttaaaatact aagaggcgaa atatccgcgg taccactctt attgataaac ttagctaatc
     8581 aattagtaaa tgttcaacat actaaataaa cactagtaat taaatatatt aaaaaacgcc
     8641 tcttggaaaa accaagagac gaaatatatc cgcgttacca ctctaattga taagataaac
     8701 ttatccactc tttctcttaa ggcgagatta cctactaaca tactaaattc aattagtatc
     8761 ctccaaagtt cttttcacta atacttattt gctaagcttc caccatcctc agctctctat
     8821 aaaacacata gaagttactc tctttctcac aggatttaaa tataaagttt tatatctctc
     8881 ttataaaata tataatagtt tatttttcca tttatgtcaa ctatataatt tattttattt
     8941 ttattttaaa gaaagcttta tataattgac taatatctca ctttccaaag acatagtcct
     9001 ttagctggtg cagtgtctga tgattggcat ctatctcttg attcaagcat atacttaata
     9061 tcttgtggag ctcttctttt tagaccaaca tctattagag cacctactat tattcttacc
     9121 atgttataca aaaatccatt tccttctaca tatatttcta taatattatc atcttctttt
     9181 atatttatag aataaatttc tcttacattt gattttttct tagatttaga agaagcaaaa
     9241 ctagtaaaat catgctctcc tactaaatac tcacttgctt ttctcattct atccaaatct
     9301 aattttttac ttacatgagt ggcatatttt cttataaaag gattgtgata cttattattg
     9361 tcaattctat acatataagt ctttgatttt acgttgtatc ttgaatgaaa cctctcatca
     9421 acttcttcaa cagcttttac aactatatct tctggtaaat attcatatag atatttttga
     9481 attttatcca aagaatccat acaattagtt ctaaaattta caacttgtcc tcttgcatgg
     9541 gctcccatat cagttcttcc agagccgatt atctccactg tttcattagt cattttactt
     9601 aagacatttt caagtttttc ttgaatagtc atattattgt cacctagttt ttgatatcct
     9661 ttatatcttg aaccatcata tgctactatc atttttatgt ttctcatctt aatcctcttt
     9721 ctaagaattt atgcttgttt agcaaactta ttttttatat taaaagttat ttttgttcca
     9781 acattctttt cactttctat tttcattctt ccaccatgaa gttttactat ttcattacta
     9841 attgctagtc ctaatccact accagcctta ttataatctt cttggaaaaa tttatctaaa
     9901 actttattta aattttgctt ttctatacct gaaccattat cctctactga tatggttgtt
     9961 atctcttcat cttgaaaagc tacaactttt atgtaaccac cttgactagt aaatttaaat
    10021 gaattttgta ttaaatttat gagaacctgt cttaatctat ttttatctcc ttggatgttc
    10081 tctataaatt cattttcaaa ttcaaacaaa agtgatatat ctttttcggc tgctttaact
    10141 ttaagttgat ttacaacacc tacgattaaa ccttcaacat caactatatc aacatgaagc
    10201 tttattctat ctgaagaaag tcttgaaaag tctaataatt cttcaactag ctttatgagt
    10261 ctttctgttt catcttgtat aatacctaaa cctaagtcta actcttctct tgttatactt
    10321 tcatagccta aggtttcact ccatcccttt atggatgtaa gaggagtcct tagttcatga
    10381 gatacagatg atataaattc ttcttttaat ttttcacttt tgtcaatctc atgtgccata
    10441 tgttcaaatg tctgtgctaa atcaccaatt tcatcatcta caatcttcat cttttctaat
    10501 tttatattat aatttcctac agccaattca tttgcaaatt tctttaactc tcgtaatggt
    10561 tttataagag tctcagcaaa tttaagactt attagtatag ctataataag tataaatatt
    10621 cctgctaata taagccatgt agcttgcttt aatatcgcat tatcaatagc atctaatgat
    10681 actgtatatc taacaacacc ttctattata ttatttactt taagtggtaa ggagatactc
    10741 ataacatgct cccctgtatc tggtattcta taagtatatg gtgttaaatt tttagttttt
    10801 atgtctttta atgcattgtt aacatcttca tagtttgctt tttctttgct tttaaagcca
    10861 tattggtcta atataatatt tttatcctta tctattattg aaattccaaa ttctgaattt
    10921 ttagttaaag gttgtttatc aaatatattt tttacttttt tctcaaaact tgtagtttcc
    10981 attgtaatag cattatattc atcagcatat tttatttcag attcaagtag ttgtttgaca
    11041 gaatcataat agtagttttg tatgtaaaac atgaacaatc cttcaaacag agtaaccatt
    11101 ataataatta ttataaaata gttttttatt actttttttc tcagaccttc aaaatgtaac
    11161 actattattc ttcctttcta aaacagtatc cataacccca aatagtttgt atatattttg
    11221 gttttgatgg gtcatcttct attttatttc ttatccttct tatatttaca tctactattt
    11281 taaaatcata taaataatta gttccccaca cttcatctaa tatttggtct ctacttaacg
    11341 attgcttagc atttgatatt agatatttca ctattgaaaa ttcagttggt gtaagctcaa
    11401 tttcctttcc atttttaaat aattttcttt tgtctatatc aagtataaat ggtggagata
    11461 ctatttcact tgatttaaca cttgaatcat cttttgctac tcttctaata agagcagaaa
    11521 ctctaactaa aagctccttt atgctaaatg gttttataat ataatcatct gcacctgaaa
    11581 taaatccttc tattttatca tcctcttgag ctttagcagt aagcattatt atacctactt
    11641 ggtcaaaatt ctcccttata aatttacata cctctatccc acttatatct ggaagcatca
    11701 catctaaaag agctattgta atatcatttt ctgttgtcat tttttctata gcttctcttc
    11761 cagtacctgc ctcaactatc tcatagcctt ctctttttaa gtttatacta acaaaacttc
    11821 taataccaat ttcatcttct agtataagta ttttttcctt cataaaataa aacctccaat
    11881 tgagtataat attaaccata tattaatgag aaatattctt ttaatgcgtc tatagtcata
    11941 tctagcttat ccatttcatt tgcattgttt acaactagta agaaagtata ttcatcgctc
    12001 tcttgaagta caatagaatt ttgagtactt gtattttttc tatcttcaag tttgttttta
    12061 ctctctacta ctatattaaa taaattctta cggtctgaat agtcaaaata gtaaaattta
    12121 aatactgcct tttctccaac aaattcttgt tcaacaagta ttttatcaaa taaattattt
    12181 ggaataaata atttaaaatt atatttataa ttataatata tctgacttgt aaatattaaa
    12241 cttgaatcag caccttcttt tccattccat ctataccaac ttatatttgc agatgcttta
    12301 gaactataag tatttctatt attacctgta ttacctgcaa ctataggtat ttctataatc
    12361 ttatctttat caatatcatc tacaggtata taataagatt tagtaatagt tttatcataa
    12421 tcactaaaag ctttgttaat ttttccatct ttcatataaa gtatctgagt aatataatta
    12481 ttatctttca atatagggat atcaagtaca atacctttta tagctgccga agaatttcca
    12541 acagacgaag caacattacc taatgttata tatagttcac ttaggttttt tacattttca
    12601 aacttaacag aatctttgat tttcatactc ccatcgaaat ttgcaacact tgcaaacatt
    12661 tcattagtct tattatttaa atgaagcatc aatatatcta acttgtcatc tccatctata
    12721 tatccaattt ttactttcat atcattaaaa tcttctttat tttgaagcca tgatgcatcc
    12781 aagtcatata tttttgttat ttcattatct actactttat atatatgcat atttgtttta
    12841 tctttggatt taactaataa gataacttcc ttatatccat ctgagtctaa atcataaaaa
    12901 ttagcatatt ctattgattc gccattttct aagaaatggt ctccaagtac gtatttttct
    12961 ccattgtaac tcaaggttac aaaccctacc tgactcacat tattacttaa atcttctttt
    13021 tgttcaaata ctactaattc atcaattcca tcgctgttta aatctacctt atttattttt
    13081 cctacttctt ttgtattaga aggcaatatc atagatgaat ctggttttaa taactggtca
    13141 ataccatcat acaatacttt ttttgtatca tcatagttag gtatcttcgc taattgttct
    13201 ggagattcag tttttatatt acacccagta acaaataaca tcatcatggc tactacagta
    13261 ataagcttta acactttcat ttaactcctc ctaatcctat aaatctttaa atttgttcac
    13321 aaaaatctcc tcacaataat tagacgtttc aaggcgtcat aatgttttac ttattttaat
    13381 aaaattttat attttacaaa tgaatcatgt aaaacttatt tattatttct aggaggctta
    13441 ggacctggat attggaaaaa gttagttttt agcataccat tatacaactt tcttttttta
    13501 tctgcttttt gcccaaactc atattcaaaa tcttcataag atgttatcaa ataatatgac
    13561 caatttttta attttctaaa tgcataacca agctccttat acaactgttt aacactatct
    13621 ttatcttcca atctttcccc ataaggagga ttagtaatta taaatccaaa ctcatcttca
    13681 cttttaaatt gcgttgcatc tcctacatta aactctatat attcatctac acctgctatt
    13741 tcagcatttt ctctagctat atctatagat tcttcatcta tatcatatcc ataaatctta
    13801 aatttagatt cattatctat cttattaaaa gcatcttttc ttacatccca ccaaattttt
    13861 ttatcaagag ttctccattt ttctgatata aactctctat ttaaaccagg tgccatattt
    13921 ataccaatca tagcagcttc aatcaatata gtaccagaac cacacatagg gtctactaaa
    13981 actctacctg ctttccatgg agttaaataa ataagcccag cagctaaagt ctctcttatt
    14041 ggagctttat ttgctttttc tctatatcct ctcttatgca aagcatctcc tgttgtatct
    14101 atagaaatag ttactttatc tttgtgtata aatacaaaaa tagggtattt ttccttatct
    14161 tctttaagca agccatcttc caaataactt ttctttaagc tttctactat agcctttttt
    14221 actatggctt gtacatctgg tgtactgtat aattttgatt ttatagagga agcttttgat
    14281 ataggaaatt gagctccata aggtatatat ctagaccagt ttattctttt tgtattttca
    14341 aataattcat caaaactttt tgcctcaaat tcagcaattt taagatgtac tctttcagca
    14401 catctcaacc acatgtttga ctttgctatt ccaaactcat cagttttata tgttattctt
    14461 ccatcttctg tttttattat ttcatatcct aaattagtaa tctctctagc cagcattttt
    14521 tccattccaa aaaaacatgg cgatattaat gtataatttt tcataaaacc tcctgtttct
    14581 ttatcttaac tataaaactt atttattttt aaaataatct acacatttaa attcatactt
    14641 cccattttat atcctgacat atctagtgta acatatttaa agccaaatat ttttaattta
    14701 gtatcaactt tattaaagtt gttattttca aaaaatttac ctaattcttc ttgtccaact
    14761 tctattctag ctatatctcc atgcattctc actctaaatt gagaaaaacc taaattggat
    14821 aaatattctt cactcttttc tattattctt aaattttcat ctgttatctc aactccataa
    14881 gggattctac ttgcaagaca agcaaatgat ggtttattaa aagtctttaa tcctaatatt
    14941 tttgaaagtg acctaatttc ttcttttttt aaaccactat cttttaatgg actaattact
    15001 cctaattcac tcagtgcctt aagcccaggt ctataatctc ctaaatcatc tatatttgtc
    15061 ccatctacta tgtactttat attatgttcc tttgctacct cttttatttt agagaatata
    15121 tattttttac aatgataaca tctatcaata ccattttctt taaattcttt taaatcaaag
    15181 ttttctatat ttaatatgat atgtttaaca ccaaaatttt gagtatattg tttggcctct
    15241 tcaatttctc tacttgaatg catcattgca tgaatagtta cagctacaac attttcacct
    15301 aatgtatctt ttgcgacctt aagcaaaaaa ttactatcta ctccaccaga ataagcaact
    15361 acaacacttc ctaattcaag taacattttt tttaatttat ctaatttttc tttttccttc
    15421 acaaaattta cctccatatc tagaaaaaca tgaactataa aactatatat tattttttac
    15481 attcttaatg attataacat ataaagtatt tattttataa attataatat caaagtattt
    15541 tttaaatctt tataaattta aacatattac tgcgctacac tgtgtataaa aacctagtgt
    15601 aacatagtaa tatgtttaaa agtcataaag aactcatcat ttacctacaa ctgaagtaca
    15661 agtttgtaac tctagtacac gaagttctaa cgatatttta tatctccttc ctcaaaccta
    15721 tgttacaata tgttaattga ttatatcaat cccaccaaaa aagcaatatt gtagagcgtt
    15781 ttaatacatc cttaagagca tttaaggttt catacccttg tataataata tcctcacaat
    15841 aaaatactaa ttcaatacat aattcatcaa attttttacc atctttaatt aaatctttta
    15901 cagaatattg aactgtatca catccaattg caattggaaa gattccatgt ttttcatacc
    15961 aatattttgc aatagctatg tgttcttcat ctctaggaca atcattaaat ccaccaatag
    16021 gtatgtatgc aaatacttcg tatggtttaa ttgttggaac tttagctatg taaattttat
    16081 tgtattcatg aaatatatct atattcataa ttggtctaaa atgtttataa tcataaaaat
    16141 catcttctaa atcaactagc tcctttaata attctgaaat ctcacgcttc ctaaaatcaa
    16201 aaaattggtt tacatcaatt tcattgtatt ttgttaaaca atactctcta taattttcaa
    16261 gagaaccata ctctttaata attatatcga tattttttct taatagccct actttttcgt
    16321 caatgactat aactggtgta tattcttttt cttttccatc ttctatcatc ttttcaaata
    16381 aaacatcaat atcaacctca ttgttcaaag gaacacattc aaaatcatat aaatcttcaa
    16441 tactcatatt tatttcctat tctttttag
//
LOCUS       DAFNNB010000083        16339 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0084, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000083 DAFNNB010000000
VERSION     DAFNNB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0084"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(201..1988)
                     /gene="aspS"
                     /locus_tag="L3M60_002230"
     CDS             complement(201..1988)
                     /gene="aspS"
                     /locus_tag="L3M60_002230"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBN6226890.1"
                     /translation="METLKGLKRTHYCGELREKNINEEVVLMGWVQKKRNLGGLVFVD
                     LRDTSGLCQIVFDTDVDKESFEKAEKLGAEFVIAVKGKVCERQSKNPNMPTGDIEIFA
                     TELRLLNKSETPPIYIKDDDDVSEALRLKYRYLDLRKPSMQRNLKLRHKVMNITRNYL
                     SNNRFCEIETPFLIAPTPEGARDYLVPSRVNPGKFYALPQSPQLYKQLLMVSGMDRYF
                     QIVKCFRDEDLRADRQPEFTQIDCEMSFVEQEDVMSMIEGLLEVIFKEVLDVELTLPL
                     PKMTYAEAMSRYGSDKPDTRFGYELTDISDVVCNCGFKVFADATQPGKSVRGINVKGK
                     ADDFTRKQISSLEEHAKTYRAKGLAWMKVGQEGVTSPIAKFFSEEEMNAILTRMNAEV
                     GDLLLFVADKNSIVFDALGQVRLEVANRLNLLDKNVYNLLWVTEFPVFEEDEETGTFS
                     AMHHPFTSPMDEDLDKLEEGDKASLRAKAYDIVLNGYEIGGGSVRISNSDVQSRMFKA
                     LGFTEERANEKFGYLLEAFKYGTPPHAGLAFGLDRLVMLLAGADNIREVIAFPKNQNA
                     VCPMTNAPTLAENEQLEELSIKVDIKDNE"
     gene            complement(2062..3324)
                     /locus_tag="L3M60_002231"
     CDS             complement(2062..3324)
                     /locus_tag="L3M60_002231"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBN6226891.1"
                     /translation="MLTKAPRGTKDITPKEAYKWRYVENKFREICALYGYEEMVTPIF
                     EHTELFKRSVGDTTDIVQKEMYSFKDKGDREITLKPEGTAGVVRAFIENKLYADTQPT
                     KLFYVTPCFRYERPQAGRQRQFHQFGIEALGSDTPSMDAEIIALAVQFFNEVGLNDLV
                     VSINSVGCPVCRKEYNALLKEYLDSKADILCDTCNERREKNPMRVIDCKNPTCKENIK
                     DIPFIADHLCDDCKSHFDKLQEYLKEMNINFVIDKTIVRGLDYYRKTAFEIISNDIGA
                     QSTVCGGGRYDGLVEQLGGPKGISGIGFGLGIERLLLTLEGNGIEIENPQSTDIFIVT
                     IGEEANTRSFKLLKDLRQNHISADKDHIERSVKAQFKYSDKINSKFTIVIGDDELKND
                     TATLKNMKTSEQTTVKLSTLVEELKQKL"
     gene            complement(3314..4870)
                     /locus_tag="L3M60_002232"
     CDS             complement(3314..4870)
                     /locus_tag="L3M60_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase"
                     /protein_id="HBN6226892.1"
                     /translation="MLYVFLKGHDYKYEVAELIKLFTSEFKFNDFNANNNMESKNLIN
                     RLIYENGVLFSSTVYYENGNLKYESIQNIENMNLEFLDGFDGNKTIEEFLNSLDNESK
                     RNFKKLCKESIKKSMFVVLKQIFNSYVPWGILTGIRPVKIVHNLMDKNLDDNSIRTIL
                     KDNYFIIDEKIDLALEIAKRERLFMYPIDKNKISLYVSIPFCPTRCVYCSFPSNSLKQ
                     FGHLKREYVYKLIEEIKGFAKIIRETNKEVETLYIGGGTPTTLDEEELDLLINSLFNE
                     LDLSKIREFTVEAGRPDTITEQKLRVLKKHNVSRISINPQTMNDDTLVKIGREHRVSD
                     LVDCFNMARRMGFDNINMDIILGLIDEDLNMVKNTLDEIKKLSPESLTVHTLAIKRAS
                     NLNINMDRYKEHLTQYEEMVKMIDLSMKYAKDMGLNPYYMYRQKHMLGNLENIGYAKE
                     GYECIYNIQIMEEKQSNYALGAGAISKFVYVDEDRIERVENVKNVEQYIERVDEMIKR
                     KKEEVYKNVD"
     gene            complement(4961..5575)
                     /locus_tag="L3M60_002233"
     CDS             complement(4961..5575)
                     /locus_tag="L3M60_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6226893.1"
                     /translation="MIIKKFVDSYFGVNTYVLGDEKTKKCAVIDPGGSLVETLSFVKE
                     NELNIEYIILTHGHGDHIGYVKDIKEKTGAKVVAHIDEKELLNDKNKNLSYTMRCGAQ
                     EFDADIYVNDKDKLELGELKMTFLHTPGHTQGCMCIRVEDEMFTGDTLFAGSIGRTDL
                     YSGNFNQMEKSLKKLCKYEDAVRVYPGHGPTSTLGVEKKTNPYM"
     gene            complement(5590..6039)
                     /locus_tag="L3M60_002234"
     CDS             complement(5590..6039)
                     /locus_tag="L3M60_002234"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBN6226894.1"
                     /translation="MRAVVQRVSSSKVTVDENTIGQINKGLLVLLGVTHDDKSSDVDY
                     MIDKILNLRIFEDENDKMNLSLMDIGGELLVVSQFTLYGDCRKGRRPGFSNAAKPELA
                     NNLYEEFVKKAKDKGVTVGTGQFAAHMMVELTNDGPVTILLDSSKSF"
     gene            complement(6064..8271)
                     /locus_tag="L3M60_002235"
     CDS             complement(6064..8271)
                     /locus_tag="L3M60_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003229747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HBN6226895.1"
                     /translation="MHDKELQEIIEKIKVYAPNANFEMVEKAYNLAKKAHEGQYRKSG
                     EPYFIHPLAVANILIEMELDIETITAGLLHDVVEDTEYTYEDIKKEFGKEVADLVDGV
                     TKLGQIKYRSKEETQSENLRKMFLAMAKDIRVILIKLADRLHNMRTLKYMPPEKAKYK
                     AKETLEIYGGIAHRLGISKIKWELEDRALRFMDPEGYYDLVSRVSMKRSQREDYIQGI
                     VELLKDKFKDLSIPCDVYGRPKHFYSIYRKMQKKHKTFEEIFDLTAVRILVDNVKDCY
                     AVLGIVHTLWRPIPGRFKDYIAMPKPNMYQSLHTTVVGPDGEPLEIQIRTHEMHNIAE
                     NGIAAHWKYKEGISSKESKVEEKLQWLRQMMEWEKDLKDPQEFMDALKEDVFNSQVYV
                     FTPKGDVIELPAESTPIDFAYRVHTNVGNKCVGAKIDGRIVPIDYKLQNGNIVEVLTS
                     SNSNGPSRDWLNIVKTPNAKSRIRQFFKKERREENVDRGNTILEREFKKHDIPQKDPL
                     VEKYMALVAKKFNQPSVEDLIATVGYGGIMSSQVVSKVKDLYLKEVKKAKKEQQIQEE
                     DLNKHNISETEYSKKRKKNASQGIIVKGLDNILVRFAKCCNPLPGDEIVGYITKGRGV
                     AIHRADCPNANNNGDFFQNRLVEVSWSSAGKGKFEAEIQIKAIDRRGIVNEITHIVAM
                     DKLSLNGINARKGKDSIVSINLLVEVNDTEELKILMKKLKAIPGVESIYRMTN"
     gene            complement(8345..8857)
                     /locus_tag="L3M60_002236"
     CDS             complement(8345..8857)
                     /locus_tag="L3M60_002236"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBN6226896.1"
                     /translation="MDLKNFIRNIDDFPKPGIDFKDVTTLFKDGDAFKYAVDSIVEEL
                     KDKGVDLVIGPEARGFLMGTPVAYALGVGFVPIRKPGKLPGEVESYEYGLEYGTDTLE
                     IHKDAIKKGQKVAIVDDLLATGGTMEAAAKLVEKLGGEVVSMQFLIELKFLNGREKLS
                     NYDVNSLIKY"
     gene            complement(8870..11320)
                     /gene="recJ"
                     /locus_tag="L3M60_002237"
     CDS             complement(8870..11320)
                     /gene="recJ"
                     /locus_tag="L3M60_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBN6226897.1"
                     /translation="MKKWILKYKGKIEESEFSKKLNISPEICQILKNRGICTEKDSEI
                     FMNPSLDYLRDPFLMKDMQKAVDRIKSAVEKNEKIWIYGDYDVDGVSSTSILCLYFKS
                     IGYHVDYYIPNRLEEGYGINEDAIKLISSRGCDLIISVDCGITSVSEVNTANDLGIDV
                     IITDHHECQNEIPVAFAVINPKQEDCNYPFDSLCGCGVAFKIIQALTPKEEFKTSMYD
                     YLEIVTLATICDIVPLIDENRIIVKNGLKSMKEGKNIGLRELIKVCGVESDKIGSSHI
                     GFAIGPRINASGRLGYSYLGVELFTTQSQEEAVEIASILKEKNNERQMIEAKMYHEAE
                     EMLKSNSRYNDDKVLVLAKEGWQHGIIGIVASKLTEKYYKPTILLSIENGEATGSARS
                     IKGFNIFEALVKCKDLMTKFGGHEQAAGLSLDSDNVEILANEINKFADYNLTEDDMIE
                     NINVEFELQENVINLNLVEELHKLEPFGLNNPNPRFIVRNYILKDLKVIGKNQQHLKL
                     NIEKEKSYECIGFNMSHLKSMYKVGDKVDVLFQLDENNYMGNRKVQFLLKDIRLARPK
                     SASNDKLSLKLMSKIIPKDTQSLYNISVSDFELFDGNTDINIFDYFEKDTLIISNSIN
                     GFYRAMSDISLIDLDFNINYNIIEDVSKNTDKLELIFSPNIDKIDLKRYNNIILYDYL
                     YNKGEYSYIYENKREESEIIKYYNKTDLLYLKNVVSNIVPSRDEFITIYKQALIKKEI
                     DLDMVNIRGTFNVIPLKFFTILNVFRELNLLDFNLNYEKNSVLIRILPKPQKKLDLNE
                     SLILNNLKNLEKQYNSSY"
     gene            complement(11333..12997)
                     /locus_tag="L3M60_002238"
     CDS             complement(11333..12997)
                     /locus_tag="L3M60_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HBN6226898.1"
                     /translation="MRISYRNLKRYREIGYVLIKYGFSFIVERLNIEGIAYKIPLFDP
                     PEEIKNMTTGERIKRVLEELGPTYIKIGQILSTRKDLLDQDIIDEISKLRDDVEKFDS
                     NIAIEIFKEEVGLSIEEIFLEFKEEPIAAASIGQVYEGILRTGEEVIVKIQRPNIEKI
                     IKSDLEILRTIANTLKDLKKDFNLDLVQMIEEFQTQLMRELDYTFEAINATKFSRIFK
                     NSDEVYIPKIYSEYNTKKILVMEKVNGTKLSDVEKIKRLGYNTKTIVEIGVRSFFTQV
                     LSHGFFHADPHPGNIFVVAKNKIAYIDFGMIGIIDNKTLNQLNEIALAGVEKNVDKII
                     YLLMEMDALNGEADIKGLRQDLLYLIHYYYDISIEKINVTDILNELFRFFRQYKIVMP
                     AQFVTLAKTVITLEGTSRTLNTDFSFGSMGKEFMKHHYKSKFNPKNVVLSSRQNIEEI
                     LLDIKTIPKQLKAILKNIERNNIKMQIEDVKMTRLENCIIELTSQISLSLVLASIIVG
                     SSLIIASPNIENNIWIKFTAIAGFFISFIIGLCLVIRSIRSKYKKD"
     gene            complement(13295..13792)
                     /locus_tag="L3M60_002239"
     CDS             complement(13295..13792)
                     /locus_tag="L3M60_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBN6226899.1"
                     /translation="MIRDYLEDKPLIDESVFVAKSADVIGNVKIGKDSSIWYNAVVRG
                     DEGPITIGENTNIQDCSIVHGDTETIIGNNVTVGHRSIVHGCKISDNVLIGMGSIILD
                     NAEIGEYTLIGAGTLITSNKKFPPGVLIMGSPGKVVRELTEEDKRYIDESYEWYLEAA
                     QNQKY"
     gene            complement(13821..15200)
                     /gene="scfB"
                     /locus_tag="L3M60_002240"
     CDS             complement(13821..15200)
                     /gene="scfB"
                     /locus_tag="L3M60_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HBN6226900.1"
                     /translation="MSMIHKFSMNGYNIVLDVNGGAVHVLDDVAYDLLDFYKEKSKEE
                     ILEILKSKYQEEKINEAYEEILNLEKEGLLYTEDTYQYHPSFVHREPVVKALCLNVAH
                     DCNLKCKYCFAAQGDFGGEKELMSFEVGKAAIDYLIANSGSRKNLEIDFFGGEPLMNF
                     EVVKQLVYYGRSVEKDYNKNIRFTITTNGVLLNDEIIDYINENMHNVVLSLDGRKEVN
                     DNMRPTLNDKGSYDITLPRFKKLVEKRAKDKYYYIRGTFTRDNLDFSKDVMHFADLGF
                     KLTSVEPVVGDESNPYALREEDLPKIFEEYEKFAVEYADRQLQGDGFKFFHFMIDLNQ
                     GPCVIKRITGCGAGNEYLSITPNGDIYPCHQFVGNEEFKMANIFDEEIVLPENLKNMF
                     REAHVYTKEECKQCWNKFYCSGGCHANAINFNNDISKPYELGCEMQRKRTECSIMIQA
                     KLMLEGATN"
     gene            complement(15500..15646)
                     /locus_tag="L3M60_002241"
     CDS             complement(15500..15646)
                     /locus_tag="L3M60_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HBN6226901.1"
                     /translation="MKKFIVRFMKFASSLALSTAILSANSTCPWIIHQPKVPKEISNL
                     KKKN"
     gene            complement(15676..16254)
                     /locus_tag="L3M60_002242"
     CDS             complement(15676..16254)
                     /locus_tag="L3M60_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBN6226902.1"
                     /translation="MFKRYAEKMTSVLICNNVIDNNESKVYSYGFEILIAFVVNIATM
                     LFIGFLFGKFTYVLFFLMCYCPIRQFSGGYHADNYFRCLLTFIFIILSTILIIENLNI
                     DLFKNIIMIIASVSWVGICALCPIEHRSNPISDREKLVYKRIAIFISTVVLLITILSL
                     SLNIFVDYFTYSTFAMFWIFIMLVLGKLKAKI"
BASE COUNT         5076 a         3029 c         1658 g         6576 t
ORIGIN      
        1 cattacaaaa gtagtttcta aaacaatcaa gtttatgtaa aatttttaaa catagtgttt
       61 taattacatt ggatgagaat acaaggataa tttatgaaaa tatatagaga gaaaatataa
      121 aatagttcta ataaaaaaaa tctgaatata aatacacttt atatccagac tttttacatt
      181 ttattttcta caactatata ttattcatta tcttttatat caacttttat gcttaattct
      241 tctaattgct cattttctgc caatgtagga gcatttgtca tagggcaaac ggcattttga
      301 tttttaggga atgcaataac ctctcttata ttatcagctc ctgcaagtaa cataacaagt
      361 ctatcaagac caaaagctag accagcatga ggaggagtac catacttaaa tgcttctaat
      421 aaataaccaa atttttcatt agctctttcc tctgtaaaac caagtgcttt aaacattctt
      481 gattgaacat cagaatttga tattctaacg cttcctccac ctatctcata accattcaag
      541 acaatatcat aagctttcgc tcttaaagat gctttatcac cttcttctaa cttatctaaa
      601 tcctcatcca taggtgatgt aaatggatga tgcattgctg agaatgtacc tgtttcttca
      661 tcttcttcaa aaactgggaa ctcagttacc catagtaaat tatagacgtt tttatccaat
      721 agattaagtc tatttgctac ttcaagtcta acttgtccta aagcatcaaa tactattgag
      781 tttttatcag ctacaaataa aagtaaatct cctacttcag cattcatcct tgtaagtata
      841 gcgttcattt cttcttcact aaagaatttt gctattggag aagttactcc ttcttgtcct
      901 actttcatcc aagcaagacc ttttgctcta tatgtcttag catgttcttc tagtgatgat
      961 atttgttttc tagtaaagtc atcagctttg ccttttacgt ttatacctct aacacttttt
     1021 ccgggttgtg tagcatcagc aaacacttta aatccacagt tacaaacaac atcagatata
     1081 tctgttaact catatccaaa tctagtatct ggtttatctg aaccatatct agacatagct
     1141 tctgcataag tcatcttagg aagtggaagt gtcaattcta catctaaaac ttctttaaat
     1201 attacttcaa gcaaaccttc aatcatactc ataacatctt cttgctcaac aaaagacatt
     1261 tcacagtcta tttgcgtaaa ctctggctgt ctatctgctc ttaagtcttc atctctaaag
     1321 catttaacta tttggaaata tctatccata ccacttacca tcaataattg tttatataat
     1381 tgtggtgatt gtggtaatgc ataaaatttt cctggattaa ctctacttgg tacaaggtaa
     1441 tctcttgctc cttctggtgt tggagctatt aaaaatggtg tttctatttc gcaaaatcta
     1501 ttatttgata agtaatttct tgtaatattc attactttat gtcttaattt taagtttctt
     1561 tgcatagatg gttttcttaa gtccaagtat ctatatttta atcttagtgc ttcagaaaca
     1621 tcatcatcat cttttatata aataggtgga gtttctgact tgtttaaaag tctaagctca
     1681 gttgcaaata tttctatatc accagttggc atattagggt ttttagattg tctttcgcat
     1741 acttttcctt ttacagctat tacaaattcc gcccctagtt tttcagcttt ttcaaaagat
     1801 tctttatcta catctgtatc aaaaactatt tgacataatc cacttgtatc tcttaaatca
     1861 acaaatacaa gaccacctaa atttcttttt ttctgtaccc atcccataag tacaacttct
     1921 tcatttatgt ttttttctct caactctcca cagtagtgag ttctctttaa accctttaaa
     1981 gtttccaaga tactacctcc attttattta attttaatgc tatatattta tctaatacac
     2041 aaaacatcat actttcttat tttacagctt ttgctttaat tcttcaacta atgtacttaa
     2101 ttttacagta gtttgctctg atgttttcat attttttaat gtcgcagtgt catttttaag
     2161 ctcgtcatct ccaattacta ttgtaaattt tgaatttatt ttatctgaat atttaaattg
     2221 cgctttaaca cttctctcta tatggtcttt atctgcactt atatgatttt gtcttaagtc
     2281 ttttaataat ttaaagcttc ttgtatttgc ttcttcacct atagttacta taaatatatc
     2341 tgtagattga gggttttcta tttcaattcc attaccttca agtgtaagta aaagtctttc
     2401 aattcctagt ccaaatccaa ttccacttat accttttggt cctcctagtt gttcaacaag
     2461 accatcatat cttcctccac cacatacagt actttgagct cctatatcat tagatataat
     2521 ttcaaaagca gtttttctat agtaatctaa acctctaact attgttttat caattacaaa
     2581 gtttatattc atttccttca agtattcttg aagtttatca aagtgagatt tacaatcatc
     2641 acacaaatgg tctgcaataa atggtatatc tttgatattt tctttacatg taggattttt
     2701 acagtctata accctcattg gatttttttc tcttctctca ttacaagtat cacacaatat
     2761 atcagctttt gaatcaagat attcttttaa taaagcatta tactcttttc tgcaaactgg
     2821 acaaccaact gaatttatac tcactaccaa atcatttaat ccaacttcat tgaaaaattg
     2881 aacagctagt gcaattattt cagcatccat tgaaggagta tcgcttccaa gtgcttctat
     2941 tccaaattgg tgaaattgtc tttgtctacc agcttgtggt ctttcatatc taaagcaagg
     3001 tgtaacataa aataattttg ttggttgagt atctgcatat aatttatttt ctataaatgc
     3061 tcttactaca ccagctgtac cttctggctt tagagtaatt tctctatcac ctttatcttt
     3121 aaaagaatac atttcttttt gaactatatc tgtagtgtct ccaacacttc tcttgaatag
     3181 ttctgtatgt tcaaatatag gtgttaccat ttcttcatat ccatataaag cacatatttc
     3241 tctaaattta ttttctacat aacgccattt ataagcctct tttggagtta tgtctttagt
     3301 tcctctagga gctttagtca acatttttat atacctcttc tttcttcctt tttatcattt
     3361 catctactct ttcaatgtat tgttcaacat tttttacatt ttctactctt tcaattctat
     3421 cttcatctac ataaacaaat ttcgatatag cacctgcacc taatgcatag ttactttgtt
     3481 tttcttccat aatttgtata ttgtaaatgc attcatatcc ttctttagca taacctatat
     3541 tttctaaatt tcccaacata tgtttttgtc tatacatata atatggattt aaacccatat
     3601 cttttgcata tttcattgat aaatctatca tttttaccat ttcttcatac tgtgttagat
     3661 gttctttata tctatccata tttatattta agttggatgc cctctttatg gctaatgtat
     3721 gaactgtaag actttcagga gaaagttttt ttatctcatc caatgtgttc tttaccatat
     3781 ttaaatcttc atctataaga cctaaaatta tatccatatt tatattatca aatcccattc
     3841 tacgagccat attaaagcaa tctactaaat cagatactct atgttctctt ccaatcttta
     3901 ctaaagtatc atcattcata gtttgaggat ttatactaat tctactaaca ttgtgttttt
     3961 ttaacactct tagtttttgt tccgtaatgg tgtcaggtct acccgcttct acagtaaatt
     4021 ctctaatttt acttaaatct aattcattaa acaatgaatt tataagcaaa tccaattctt
     4081 cctcatcaag tgtagttgga gttccaccgc caatatatag agtttcaact tctttattag
     4141 tctccctaat aatcttggca aaccccttaa tttcttcaat caatttataa acatattctc
     4201 tttttaaatg tccaaattgc tttaatgagt ttgaaggaaa tgaacaatat acacatctag
     4261 ttgggcaaaa tggaatactt acataaagag aaattttatt tttatctata ggatacataa
     4321 ataatcgctc tctcttagct atttcaagag ctaaatctat tttctcatct attataaaat
     4381 aattatcttt tagaatggtt cttatgctat tatcatctag atttttatcc attagattgt
     4441 ggacaatttt gactggtcta ataccagtca aaattcccca tggtacataa gaattaaata
     4501 tttgtttaag tactacaaac atgctttttt tgatactttc tttacacagc tttttaaagt
     4561 tcctcttact ttcattatct aagctattga gaaattcttc aatcgtttta ttcccatcaa
     4621 atccatctaa aaattcaaga ttcatatttt ctatattctg tattgattcg tattttaagt
     4681 ttccattttc atagtataca gtacttgaaa ataatacacc attctcataa atcaatctat
     4741 ttattaaatt ctttgactcc atattattat tggcattaaa gtcgttaaac ttaaattcac
     4801 tggtaaacaa ttttataagt tctgctactt catacttata atcatgtcct tttaaaaaaa
     4861 catacaacat caacttaact ccttctattt aacagtagaa gaaccccatc actaatacag
     4921 ttattaaata ttttatactt ttttaaatac taatataaaa ttacatgtaa ggatttgtct
     4981 tcttttcaac ccctagagta ctagttggtc catgtccagg atatactcta acagcatctt
     5041 catatttaca taatttttta agtgattttt ccatttgatt aaagtttcca ctatacaaat
     5101 cagttctacc aatacttcca gcaaacaaag tatctcctgt gaacatttca tcttcaactc
     5161 ttatacacat acatccttga gtatgtcctg gagtatgcaa gaaagtcatt tttaattcac
     5221 caagttcaag cttgtcttta tcattcacat atatgtctgc atcaaattct tgtgctccac
     5281 atctcattgt ataacttaga tttttatttt tgtcatttaa taattctttt tcatcaatat
     5341 gtgcaactac cttagcacca gttttttctt taatatcctt aacatatcct atatggtctc
     5401 catgcccatg agtaaggatt atatattcaa tatttaattc attttctttt acaaaactta
     5461 atgtctctac taaacttcca cctgggtcta taactgcaca ttttttagtc ttttcatcac
     5521 ctaatacata agtatttact ccaaagtaac tatctacaaa tttttttata atcattaaag
     5581 ttttccccct taaaaagatt tacttgagtc taatagtatt gttactggac catcgttagt
     5641 taactctacc atcatatgag ctgcaaattg acctgtacca actgtcacac ctttatcttt
     5701 tgcttttttt acaaattctt cataaaggtt atttgcaagt tctggtttag ctgcattact
     5761 aaatccaggt cttcttcctt ttctgcaatc tccatataaa gtaaattgag atactactaa
     5821 aagctctcca cctatatcca ttagagataa attcatcttg tcattttcat cttcaaaaat
     5881 tctcagattc aatattttgt caatcatata atctacatca cttgatttat catcatgagt
     5941 cactcctaat aatactagta accccttatt tatttgacct atagtgtttt catctactgt
     6001 aactttagat gaagatactc tttgtactac tgctctcata gcttcctccc tattatttta
     6061 actctaattt gtcattctat aaatactttc tacaccagga attgctttta attttttcat
     6121 tagaattttt aattcctctg tgtcattaac ttctacaagt aagtttatac ttacaatact
     6181 atccttacct tttcttgcat ttattccatt taaactaagt ttatccattg caacaatatg
     6241 agttatctca ttaacaattc ctcttctgtc aatagctttt atttgaattt ctgcttcaaa
     6301 cttacccttt cctgctgaac tccatgaaac ctccaccaat ctattttgga agaaatcgcc
     6361 attattgttt gcatttgggc aatcagctct gtgtatggcc actcccctac ctttagtaat
     6421 atatcctaca atttcatctc ctgggagagg attacaacac ttagcaaatc ttactaagat
     6481 attgtcaagc ccttttacta taattccttg tgatgcgttt ttctttctct ttttactgta
     6541 ttctgtttca cttatattgt gtttatttaa atcttcttct tgtatctgtt gttctttttt
     6601 agctttttta acctctttta aatacaggtc ttttacttta gatacaactt gtgatgacat
     6661 tataccacca taacctacag tggcaattaa atcttcaaca cttggctgat tgaatttctt
     6721 agcaactaat gccatatatt tttcgacaag agggtctttt tgcggtatat catgcttctt
     6781 aaattctctt tctaatattg tattcccacg gtcaacattt tcttctcttc tttccttttt
     6841 gaaaaattgt ctaattctac tctttgcatt tggcgtcttc actatattga gccaatctct
     6901 acttggtcca ttagaattag atgatgtaag cacctcgaca atattaccat tttgaagctt
     6961 ataatcaata ggaactattc ttccatcaat tttagctcct acacatttat tacctacatt
     7021 tgtatgaact ctataggcaa aatctatagg tgttgattct gctggtaatt caattacgtc
     7081 tccttttggc gtaaatacat aaacttgact attaaataca tcttctttaa gtgcatccat
     7141 aaattcttgt gggtctttta aatctttttc ccactccatc atctgtctca accattggag
     7201 tttttcttca acttttgatt ctttgctaga gataccttct ttatatttcc aatgggctgc
     7261 tataccattt tcagctatat tatgcatctc atgagttctt atttgtatct ctaaaggttc
     7321 tccatcagga cctacaacag tagtgtgtaa tgattgatac atatttggtt ttggcattgc
     7381 tatataatct ttaaatcttc ctggaatagg tctccataat gtatgaacta taccaagtac
     7441 tgcataacaa tcctttacat tatcaaccaa tatccttact gccgttaaat caaaaatttc
     7501 ttcaaaagtc ttatgttttt tttgcatctt tctatatata ctgtaaaaat gctttggtct
     7561 accatataca tcacatggta tgcttaaatc tttaaactta tctttaagaa gttcaacaat
     7621 tccttgaata taatcttctc tttgacttct cttcattgat actctactaa ctaaatcata
     7681 atacccttct gggtccataa accttaatgc tctgtcttct agttcccatt ttatcttgga
     7741 aatccctaat ctatgagcta tacctccata aatttcaagc gtctcttttg ctttatattt
     7801 tgctttttct ggaggcatgt attttaatgt tctcatgttg tgcaatctat cagcaagctt
     7861 tataagtata acccttatat cctttgccat tgccaaaaac atttttctta aattttcaga
     7921 ttgagtttct tcttttgaac gatattttat ttgaccaagt ttcgttactc catcaactaa
     7981 atccgctact tctttgccaa attctttttt tatgtcttca taggtatatt ctgtatcttc
     8041 tacaacatca tgaagtaaac ctgctgttat agtttcaata tctaattcca tctctattaa
     8101 aatatttgca actgctaatg gatgaataaa gtatggttcc ccagattttc tatattgtcc
     8161 ttcatgcgct ttttttgcaa gattataggc tttttcaacc atttcaaaat ttgcattagg
     8221 tgcataaact tttatttttt caattatttc ttgtaattct ttatcatgca tataaccacc
     8281 tattttgtat aaaattttaa tatatatgaa aatagttggt gttataccaa ctattataaa
     8341 agtactagta ttttataaga gaattaacat catagttaga caatttttct ctcccgttta
     8401 agaattttag ctcaatcaaa aactgcatag aaactacttc tccacctaat ttttctacaa
     8461 gcttagcagc agcttccata gttccaccag tagccaataa atcgtctact attgctactt
     8521 tttgaccctt tttaattgca tccttatgta tctctaaagt atctgtacca tactctaatc
     8581 catactcata actctcaact tcacctggta actttccagg ttttctaatt ggaacaaatc
     8641 ctactcctaa agcatatgct acaggtgtac ccatcaaaaa tcctcttgct tctggtccta
     8701 ttactaaatc tacaccttta tctttcaatt cttcaactat agaatctact gcatatttaa
     8761 atgcatctcc atctttaaat agagttgtca catctttaaa atctatacct ggctttggga
     8821 aatcatcaat attccttata aagtttttta aatccatggg aaacgcctcc taataactgc
     8881 tattgtattg tttttctaaa ttttttagat tattgagtat taaactctca tttaagtcta
     8941 attttttctg tggttttggc aatattctta tgagtacaga atttttttca taatttaaat
     9001 taaagtcaag taaatttaat tctctaaaca catttaatat cgtaaaaaac tttaatggaa
     9061 ttacattaaa agttcccctt atatttacca tatccaaatc aatttctttt tttatgagtg
     9121 cttgtttata aattgttata aattcatctc ttgatggaac tatattacta actacattct
     9181 ttaaatataa taggtctgtt ttattataat attttataat ttcactttct tctcttttat
     9241 tctcatatat atacgaatat tcgcctttat tgtataaata atcataaaga ataatattat
     9301 tatatctttt taagtctatt ttatcaatat taggagaaaa tataagctct aatttatcag
     9361 tatttttgct aacatcttca ataatattgt aatttatgtt aaaatccaaa tctataagtg
     9421 atatatctga cattgctcta taaaatccat ttatagaatt agatattatt agtgtatctt
     9481 tttcaaaata atcaaatata tttatatctg tatttccatc aaataattca aaatcagata
     9541 cagatatatt atacaagctt tgagtatctt ttggtattat ttttgacatt aatttcaatg
     9601 aaagtttgtc attacttgca cttttaggtc gagccaatcg aatatcttta agtaaaaatt
     9661 gtactttcct atttcccata tagttatttt catctaattg aaacaataca tccactttgt
     9721 ctcctacttt atacattgat tttaaatggc tcatattaaa tcctatacat tcatagcttt
     9781 tctctttttc tatgtttaat ttcagatgtt gttgattctt acctataact ttcaaatctt
     9841 ttaaaatgta atttctaact ataaatctag gattaggatt atttaatcca aaaggttcta
     9901 acttatgtaa ttcttctaca agatttaaat ttattacatt ttcttgtaat tcaaactcaa
     9961 catttatatt ctctatcata tcatcttcag ttaaattata atctgcaaat ttatttattt
    10021 catttgctaa aatctctaca ttatctgaat ccaaagataa tcctgctgct tgttcatgac
    10081 caccaaactt agtcatcaaa tctttgcact ttacgagagc ttcaaatata ttaaatcctt
    10141 ttattgacct tgctgaacca gtagcttctc cattttctat acttagaaga attgttggct
    10201 tgtagtattt ttcagttaac tttgatgcta caattcctat tattccatgt tgccaacctt
    10261 ctttagctaa aaccaacact ttgtcatcat tgtatctgct attagacttt agcatttctt
    10321 cagcttcatg atacatctta gcttctatca tctgtctttc attatttttt tctttcaata
    10381 tactagctat ttctactgcc tcttcctgtg attgagtagt aaatagctct actcctaagt
    10441 aagaataccc caatcgacct gaggcattta tcctaggacc tattgcaaaa cctatatgag
    10501 aagaacctat cttgtcactt tctacgccac agacttttat aagctccctt aatccaatat
    10561 ttttaccttc tttcattgat tttaaaccat tttttacaat tattctattt tcatctataa
    10621 gtggaacaat atcacaaata gttgctagtg ttactatttc taaataatca tacatactag
    10681 ttttaaattc ttcttttgga gtcaaggctt gaattatttt aaatgctaca ccacatccac
    10741 atagtgaatc aaatggataa ttgcaatctt cttgttttgg atttattact gcaaaggcta
    10801 ctggaatttc attttgacat tcatgatggt ctgtaattat aacatctatg cccaaatcat
    10861 ttgctgtatt gacctcagac actgatgtaa ttccacagtc aacacttata atcaagtcac
    10921 atcccctaga gcttataagc ttaatagcat cttcatttat accatatcct tcttctaatc
    10981 tattaggaat atagtaatca acatggtaac ctatactctt aaaatataag cacaagatag
    11041 atgtagatga tacaccatca acatcataat ctccataaat ccaaattttt tcgttcttct
    11101 caactgctga ctttattcta tcaacagctt tttgcatgtc tttcattaaa aaagggtctc
    11161 ttaaataatc taaagatgga ttcataaata tttctgaatc tttttcagta catatccctc
    11221 tattttttaa aatttggcag atttccgggg aaatattaag ttttttgcta aattcacttt
    11281 cctctatctt acctttatat ttcaatatcc attttttcaa atttaattcc ccctaatctt
    11341 ttttgtattt tgacctaata cttcttataa ctaaacaaag acctattata aaggatataa
    11401 agaaacctgc tatcgcagta aactttatcc atatattatt ttctatattt ggagatgcaa
    11461 ttataagaga agaacctact ataatggatg ccaacactaa actcaaagat atttgagatg
    11521 ttaactctat tatacaattt tctaatcttg tcattttaac atcttcgatt tgcattttta
    11581 tgttatttct ttctatattt tttaaaattg cttttaattg ctttggaata gtttttatgt
    11641 ccagcaatat ttcttctata ttttgtcttg aacttaaaac aacattttta gggttaaatt
    11701 tactcttata atgatgtttc ataaactctt ttcccataga gccaaaagaa aaatctgtat
    11761 ttaaagttct acttgttcct tctaatgtta tgactgtctt ggcaagagtt acaaattgtg
    11821 caggcatgac tattttgtac tgtctaaaaa atctaaatag ttcatttaat atatctgtta
    11881 catttatttt ttctatagaa atatcatagt aataatgtat tagataaagt aaatcctgtc
    11941 taagtccctt aatatcagcc tctccattta atgcatccat ttccatcaat agatatatta
    12001 tcttatcaac attcttttct acacctgcta atgcaatttc atttaattgg tttaaagttt
    12061 tattatcaat aattccaatc attccaaaat ctatatatgc tatcttgttt ttagcaacta
    12121 cgaatatatt acctggatgt gggtctgcat gaaaaaatcc atgagataat acctgtgtaa
    12181 aaaatgacct aactcctatt tcaacaatag tttttgtgtt ataacctagt ctctttattt
    12241 tttctacatc acttaacttt gtcccattaa ctttctccat tactaaaatt ttcttagtat
    12301 tatattctga gtatatttta ggtatatata cttcatcgct atttttaaat atcctagaga
    12361 atttagttgc atttatagct tcaaatgtgt aatcaagttc tctcattaat tgagtttgaa
    12421 attcttcaat catttgtact aaatctaaat taaaatcttt ttttaaatcc tttaaagtat
    12481 ttgcaatagt tctaagaatc tctaaatcag attttattat tttttctata tttggtcttt
    12541 gaatttttac aattacttct tctcctgttc taagtatacc ttcataaact tgaccaatcg
    12601 aagcagctgc tattggttct tctttaaatt ctaagaatat ttcttctata cttaaaccca
    12661 cttcttcttt aaatatctca atagctatat ttgagtcaaa tttctctaca tcatctctga
    12721 gttttgatat ttcatcaatt atatcttggt ctaacaagtc ttttcttgta ctcaatatct
    12781 gtcctatttt tatatatgta ggtcctagtt cttctaaaac tcttttaatt ctctcaccag
    12841 ttgtcatatt ttttatttct tctggtgggt caaatagtgg tattttatag gcaatacctt
    12901 ctatatttag tctttctaca ataaaactaa aaccatactt tattaaaaca tatccgattt
    12961 ctctatatcg ctttaaattt ctataactta ttctcaaatc ttttcactcc taagataggt
    13021 atacccaaaa acttacacct taatttatcg tatatgacat tatttcacca attgatattc
    13081 taatatctta tatactcttt aatatttaat tttatcacat atactagcaa ttttattaaa
    13141 attatttaac tcagttgatt tttatttttg acattttata tctgatttaa acaaatactt
    13201 tttaaaaact aaaatatcaa caaaatctaa taaaataaat tagtggttgc ctcaaaacag
    13261 atttaaatat gttttgaaac aaccactctt taaactaata tttttgattt tgtgctgcct
    13321 ctaaatacca ctcataagat tcatcaatat atcttttatc ttcttctgta agctctctaa
    13381 caactttacc aggagagccc attatcaaaa cacctggtgg aaacttctta ttagatgtaa
    13441 ttagcgtacc tgctcctatc agagtatatt ctcctatttc agcattatcc aatattattg
    13501 aacccatacc aattaataca ttatcactaa ttttacaacc atgaactatt gacctatgcc
    13561 caactgttac attgttacca attatagttt ctgtatctcc atgcactata gaacagtctt
    13621 gtatattagt attttctcca atagttattg ggccttcatc acctctaact accgcattgt
    13681 accatatact tgaatctttg cctattttaa catttccaat tacatctgct gattttgcca
    13741 caaatacact ttcatcaatt aaaggcttat cttctaaata atctctaatc atatataaac
    13801 aactccttta aaatataaat ttagttggtt gctccttcta acatcaactt agcttgtatc
    13861 attatagagc actctgtacg ctttctttgc atttcacatc ccaattcata tggctttgat
    13921 atatcattat taaagtttat cgcatttgca tgacatccac ctgaacagta aaacttattc
    13981 caacattgct tacactcttc tttagtatat acatgcgctt ctctaaacat attttttaaa
    14041 ttttctggaa gtacaatttc ttcatcaaat atatttgcca ttttaaattc ttcatttcca
    14101 acaaattgat gacatggata gatatctcca tttggagtta tagaaagata ctcattccca
    14161 gccccacatc cagttattct ctttattaca catggacctt gatttaaatc tatcataaaa
    14221 tggaaaaatt taaatccatc tccttgtaat tgtctatctg catactctac agcaaatttt
    14281 tcgtattctt caaatatctt aggtaaatct tcttctctaa gtgcatatgg attgctctca
    14341 tctccaacga ctggctctac agaggttaat ttaaatccta aatctgcaaa atgcataaca
    14401 tcttttgaaa aatctaaatt atctcttgta aaagtacctc ttatatagta atacttatct
    14461 tttgctcttt tttccacaag ttttttaaat cgtggtaatg taatatcata acttccttta
    14521 tcatttaaag ttggtctcat gttatcatta acttcttttc ttccatctaa acttaagact
    14581 acattgtgca tattttcatt tatataatct ataatctcat catttaacaa tacaccattt
    14641 gtagttattg taaatcttat atttttatta taatcttttt ctacacttct tccataataa
    14701 accagttgtt ttacaacttc aaaattcatt aaaggctctc caccaaagaa atctatctct
    14761 aagttctttc tacttcctga attagcaatt aaatagtcaa ttgctgcctt accaacttca
    14821 aaactcataa gctctttttc tcctccaaaa tcaccttgag ctgcaaaaca gtatttacat
    14881 tttaaattac aatcgtgagc tacatttaaa cataaagctt ttactacagg ttctctatgt
    14941 acaaaacttg gatgatattg atatgtatct tctgtatata aaagaccttc tttttccaaa
    15001 tttaatattt cttcatacgc ttcatttatt ttttcttctt gatattttga ttttaatatt
    15061 tctaatattt cttctttact tttttcttta taaaaatcta acaaatcgta tgcaacatca
    15121 tctaacacat gaacagctcc accattcaca tccaaaacga tattatatcc attcatagaa
    15181 aatttatgta tcatactcat accttacctc ctaaattact atctatacta aagtttttta
    15241 ttattttaat taaactttac ccttcattat atcataagtt tacccaaaat acatacaata
    15301 caaaaatgac ttactcaccg taagtcattt cattgaatac actatatccc cctaaattgg
    15361 cgcactaata tattatcaaa taggtattag gctcctataa aataaataaa ataaattttg
    15421 tatctgattt tatcttgttt taatcttatc aaaatataat aatttttttt aataattttg
    15481 tataaataat tgcttgtatt taatttttct tctttaaatt gctgatttct ttgggtactt
    15541 taggttgatg aataatccaa ggacaggtac tattagctga caaaatagca gtgctaagtg
    15601 ctaatgagct agcaaatttc ataaatctta caataaactt tttcataact taattctccc
    15661 tcatacatat tttacttata ttttagcttt taacttacct aatactaaca taataaaaat
    15721 ccaaaacatg gcaaatgtgc tatatgtgaa ataatctaca aatatattca gagaaagact
    15781 taaaattgtt atcaataaca ctactgtaga tataaatata gcaattctct tataaaccaa
    15841 cttttctcta tcacttatag ggttgcttct atgttctatt gggcataggg cacaaattcc
    15901 aacccaacta acacttgcaa taatcataat aatattttta aataagtcta tatttagatt
    15961 ttctataatt aagattgtag ataaaattat aaaaataaag gttaataagc atctaaaata
    16021 attgtctgca tgataaccac ctgaaaattg ccttattgga caataacaca ttaaaaaaaa
    16081 caatacatat gtaaatttac caaataaaaa acctataaac agcatggtgg ctatattaac
    16141 tacgaaagct attaatattt caaatccata agaatagact ttagattcat tattatctat
    16201 cacattatta caaattaaaa cagatgtcat tttttcagca tatcttttaa acatttttac
    16261 ctccaaaaaa attttacata tatagagtac attctttttt gagaaaattc aatatacttt
    16321 acaaaactag ttgttaaaa
//
LOCUS       DAFNNB010000084        16246 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0085, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000084 DAFNNB010000000
VERSION     DAFNNB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16246
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0085"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..542)
                     /locus_tag="L3M60_002243"
     CDS             complement(<1..542)
                     /locus_tag="L3M60_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6226903.1"
                     /translation="MICLNKRQENLLTTLLLKEEFINVNEIANDFECSERTIRNDCKY
                     IDEWLSTFCNACIKRKPNIGIKFSGDGTDKILVNQKLRGEVKRVNSELHKQVEILNLL
                     LCNNKKVTLNDLSNKLYINKNIVREEIGKLSVALNNYNLNISTKKGSGIYIEGDEKDI
                     RIMLVSFLFKYIDKYRLNID"
     gene            complement(874..1656)
                     /locus_tag="L3M60_002244"
     CDS             complement(874..1656)
                     /locus_tag="L3M60_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HBN6226904.1"
                     /translation="MKICNDNFIKDFKNMINKCLDFIYPENISCIICDKSIKKTNTYS
                     ICKSCFKEMNFIQDGCMKCGKPIIHHSIEKEFIEECSYCFNKDFYFDKSISCIEYNDV
                     TKKIVLGLKYNQKTFMAKYIAQIMKEKLYLENIKFDYVLFVPLHKKRLNKRGFNQAQK
                     IAFNLSKIVNIPLLDCISRKKYTRMLYKLNKKERKEELKNVFVVKENIKLINNKNILL
                     IDDIFTTGLTTNEISKLLKLSGANKVFVLTLLTKANDNYIME"
     gene            complement(1659..3878)
                     /locus_tag="L3M60_002245"
     CDS             complement(1659..3878)
                     /locus_tag="L3M60_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HBN6226905.1"
                     /translation="MEKLEGMISEIVFKNEDNGYTIAHLVNENDEIVVVGCMPTLAIG
                     ESIEVEGKWVNHKVYGTQFEVNSFMPVTPSSLEGIYVYLSSGMIHGIGEKMAKRIIDK
                     FGVDTLEVIQNSPEKLQEVEGIGSKKVKQIVKSYEEDRELRNIIIQLSPFGITPNYCL
                     KIYKKYKSSAIDVINKNPYQLAEDIRGIGFKVADSIANKIGIDKNSKDRICQGILYTL
                     NKSLSNGHTYLPEHVLIQDSEKLLELSGKIIKECVMMLVYNQKIHIEKVNNENLIYLM
                     PYYLAENGVCSQIVKLSQYEFEDLKIDIDNEINVLEENKKIKLAEKQILAVKESVNSG
                     VLIITGGPGTGKTTTINAIIDIFENNGKSVTLAAPTGRAAKRMSETSNKEAKTIHRLL
                     EMGFSTDDDLTFFKDEEDPINSDVIIVDEVSMVDIILMYNLLRAIKLGTRVILVGDSD
                     QLPSVGAGNVLKDMISSNIINVVKLNEIFRQAQESMIIVNAHKINNGEPLYLNTKGKD
                     FFFIRKSTNEEILNEIIGLVNERLPKFYKVDKLKDIQVLSSMRKGELGVTNLNIELQK
                     YLNKKEKFKVEESFSKRLFRVGDKVMQVKNNYTKKWETEDQKESGEGIYNGDIGYVYH
                     IDKDKKTIYVLFDQTKIVSYLYDELDEIDHSFCTTIHKSQGSEFPVVVLPITWAPPML
                     LSRNLLYTAVTRAKKLVVLVGDVKYLEYMIKNNRVNQRYSNLGYKLNKFKQEGLLIE"
     gene            complement(4142..5239)
                     /locus_tag="L3M60_002246"
     CDS             complement(4142..5239)
                     /locus_tag="L3M60_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226906.1"
                     /translation="MSGKVNIKTVISFAGAYVATVIGSGFATGQEILQFFTFYGYAGI
                     VGGIISMVLFSWFGAEVIDKGRELKLKEPIKIYQVYCGKYLGIFFEWFGPLFLFGTLV
                     IMIAGAGATLSEYYGLNPYVGRIGMAIVSLITVSLGLTRLSKILGNIGPIIIIFTLLV
                     GAISLFSNIDALSNAGNMVDSLNIKTATSNGYFSGVLYTCYNVIIVITFLTGMGASAV
                     NKKDAVWGGIVGGVALMAAAIMMNLALLSDIGNIYTKEIPALYLADKISPIIGILFSV
                     VLLLGIYTTAVPLLWSVTNRFVEDDHPKFKIITIVVSILACIGGLLPFDKLVGTLYPY
                     TGYMGILILLCILYRRITKTEGYKENKSEIS"
     gene            complement(5552..6745)
                     /locus_tag="L3M60_002247"
     CDS             complement(5552..6745)
                     /locus_tag="L3M60_002247"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBN6226907.1"
                     /translation="MARHLFTSESVTEGHPDKICDQISDSILDALLEKDPQSRVACET
                     TVTTGLVLVAGEISTSAYVDIPKLVRETVREIGYTRAKYGFDCDTCAVITSIDEQSGD
                     IAMGVDEGLESKTGEEIEEEIEKVGAGDQGIMFGFACNETPELMPLPISLAHKLSRRL
                     TEVRKTGIVDYLRPDGKTQVTVEYEGNKAVRVHTVLISAQHCETVSNDKIREDLINHV
                     IKEVIPAELLDEDTKIYINPTGRFVIGGPQGDTGLTGRKIIIDTYGGYSRHGGGAFSG
                     KDPTKVDRSAAYAARYVAKNIVASGLADKCEIELAYAIGIARPLSIFIDTFGTGKVSE
                     EKLVELVNKHFDLRPGAIIRDLDLRKPLYKKVAAYGHFGRTDIDLPWERTDKVEQLRK
                     DALGE"
     regulatory      complement(6881..6984)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            7173..7682
                     /gene="yyaC"
                     /locus_tag="L3M60_002248"
     CDS             7173..7682
                     /gene="yyaC"
                     /locus_tag="L3M60_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HBN6226908.1"
                     /translation="MYLAKVNYSDDDVIQMLSSVLKTIINENTIVVCIGTDRAIGDTL
                     GPLVGTILKNSNFKYPVYGTLDNPIHALNIYESLDTIKNTHIQGNFLAIDACLGSQSN
                     IGNIQIREGPILPGKGVGKKLPQIGNYSIVGIVDKIDENNKLSFNNIRLSFILDLAET
                     IALALLVST"
     gene            complement(7793..8221)
                     /gene="fabZ"
                     /locus_tag="L3M60_002249"
     CDS             complement(7793..8221)
                     /gene="fabZ"
                     /locus_tag="L3M60_002249"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBN6226909.1"
                     /translation="MLNIDEIKKLIPHRYPFLLVDKITELEVGKRAVGIKNVTVNEPF
                     FQGHFPEYPLMPGVLIVEALAQVCGVAMMSVEENKGKLGVFAGIDKVRIKREVRPGDT
                     LTMEVEMTTLRKNIAKADAKAYVGEELVCKGELMFALVEK"
     gene            complement(8237..9256)
                     /locus_tag="L3M60_002250"
     CDS             complement(8237..9256)
                     /locus_tag="L3M60_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HBN6226910.1"
                     /translation="MAGADIGIDLGTANVLVYVSGKGIVLEEPSVVAIDKRTDSVLAV
                     GEEAKKMIGRTPGNIVAIRPLRDGVISDYDVTEKMLKSFIDKIVDKKGFGRFFMPRIM
                     VCVPTGVTEVEKRAVEDATRQAGAREVYIIEEPIAAAIGAGVDISQPNGNMIIDIGGG
                     TVDIAVISLGGAVVSESIKVGGDRFDDAIVKYMKKQHNLLIGERTAEKIKFEIGSAFK
                     REEEKYMKITGRNLITGLPSSITINSTEMLEALRECVEQIVVATHAVLEKTPPELAAD
                     IGDVGIIMTGGGSLLYGLDKIIEQRTGITVTIADEPLSCVAKGTGIALGSIDLLETGG
                     SFKRK"
     gene            complement(9453..9713)
                     /gene="spoIIID"
                     /locus_tag="L3M60_002251"
     CDS             complement(9453..9713)
                     /gene="spoIIID"
                     /locus_tag="L3M60_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HBN6226911.1"
                     /translation="MRSHIEERAIVVAKYILEKNTTVRQTAKTFGVSKSTIHKDVTER
                     LKEINPSLAKEVKNVLDKNKSERHIRGGLATKLKYEKEHKKM"
     gene            complement(9811..10479)
                     /locus_tag="L3M60_002252"
     CDS             complement(9811..10479)
                     /locus_tag="L3M60_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HBN6226912.1"
                     /translation="MKKKLLEKDGFYLSLFVCVCLLAVGGVWFTNNNVDKLASNKGIM
                     ENANKDSEEEIHLIEKDKKDAIPTATDSKQNLEKAKSKEENKSSTTKLNYIGDKVIRG
                     YSEKEPSYSKTLDVWETHKGVDISCTKGKEVKSLLNGIVVDVFNDEEYGQSVKIKSDN
                     NIVVVYSNLDKNVNVKKEQKITEGQSLGTVGSTSQIESEEGIHVHLEAYSGEKSIDPM
                     SLIK"
     gene            complement(10545..11609)
                     /gene="spoIID"
                     /locus_tag="L3M60_002253"
     CDS             complement(10545..11609)
                     /gene="spoIID"
                     /locus_tag="L3M60_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HBN6226913.1"
                     /translation="MKNPLIVLLGFVTCSVLVPSLITLVSYKNVELTEKPESPVSINK
                     TIKKSDIKDKSNKEEKNIVNYETVNKKAPIINVYNHITGKTEKMDMENYLCGVLAGEM
                     SSEFDIEALKAQSVAARTYVVYKQEHGKSSKHKNAVVCTDYKHCQEYKSYDTLKKLNG
                     EEWIKNKYSKIQEAVRGTKGQIITYNDKAILPLYFSTSSGKTENSEEVFSAKYPYLKS
                     VESPYDKYSPKFASTLKISNTDFVKSLRRAYSTIVIDVNNLSKQVSITKRSDAGTVEK
                     IKLGNKELTGKDIRTVFKLNSANFDIKFGEGYIDFVVKGYGHGVGMSQWGAEGMAEEG
                     YKYYDILSHYYTDTKIKDIY"
     gene            complement(11696..12949)
                     /gene="murA"
                     /locus_tag="L3M60_002254"
     CDS             complement(11696..12949)
                     /gene="murA"
                     /locus_tag="L3M60_002254"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBN6226914.1"
                     /translation="MAKIIVKKSNPLKGSVKIDGAKNAVLPIIAATLLANGKSTLNGV
                     PNLRDVHVISDLLRHVGAEVEYKENTLTVDASNIKTCEAPYELVRKMRASFLVMGPLL
                     ARFNSTKISMPGGCAIGTRPIDLHLKGFKALGAKIEMDHGFVEAATEKLVGNKLYLDF
                     PSVGATENIMMAASLAEGTTIIENAAEEPEIVDLANFLNEMGADVKGAGTNTIKIKGV
                     KELKGAEHNVIPDRIEAATYMVAAAMTKGDITVENVLMEHLKPVVAKLREAGCEITEM
                     DNSVRVVGPKVLKPIDIKTLPHPGFPTDVQAQFMAMLTVANGTGVVIETVFENRFMHV
                     AEFNRMGANIKIDGRSAVVNGVDELHGAAVNATDLRAGAALILCGLIAEGETQIGEIY
                     HIQRGYVDIDKKITALGGQIEIVED"
     gene            complement(12970..13707)
                     /locus_tag="L3M60_002255"
     CDS             complement(12970..13707)
                     /locus_tag="L3M60_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HBN6226915.1"
                     /translation="MKRVKLAISFFILMIIGMTISYADTSINTRYNRLSKALEISEAN
                     FKFYNIKINSVINHNLSNKEFYNIFIEIINNLKLDKKGINWNRKGSGDKIQLCATIKG
                     SDKEISIKGINKNNKESYIVVDILDNKVYKHKEVIYSNVENVLNKYSSQVDVSACIVG
                     EYTKRLQNNKYDDILQKILYNMSAKQIDEVKDVNFLSITAYSKVLNDYELEYLGEKIN
                     LNIGIKYNEDDGRTLIYIATPIIKLDY"
     gene            complement(14157..15452)
                     /locus_tag="L3M60_002256"
     CDS             complement(14157..15452)
                     /locus_tag="L3M60_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase"
                     /protein_id="HBN6226916.1"
                     /translation="MANDFKKEKKEDYFVNLKAISTEVLQEKVQDNAWVIVDTRLNDA
                     YNGWKLDGVKRGGHIKGAVDFSANWLSVYSDKKDEVLEQALKTKRIDIDKNIVLYDAN
                     GKDALVVADYLSKKGYKYLYKYDIKQWADNENLPMERYKNYQMIVPAFIIKDILDGKI
                     PETFENSKNIKMIEASWGEESYTKGHIPTSVHVNTDIIEPPPTWMLDNDDNLTKFALD
                     YGFTKDDTVIVSSSTPMASYRLAVILRYIGVKDVRVLNGGTNSWLSAGYELEFTSNPK
                     HSCTNFGADIPVNSQLVVTTSELRQKLKEKNKFVLVDNRTWDEYIGKVSGYTYYDKKG
                     RIPGALYGHSGSDSVSLEEYRNIDNTMRNKYEILEMWDKENIDVNKQLIFMCGSGWRA
                     AEVLTYANVIGVENTSLYSDGWMGWSLDNSNLIEVGEHK"
     gene            complement(15944..>16246)
                     /locus_tag="L3M60_002257"
     CDS             complement(15944..>16246)
                     /locus_tag="L3M60_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBN6226917.1"
                     /translation="LIEKGTPVIAIATQEKLFEKMVSNMEEVRARGAYVVAIAQSHNK
                     DVEKAADKIIYIPNSDDILSPILAVVPMQLLAYHVSVLRGCDVDKPRNLAKSVTVE"
BASE COUNT         4945 a         3014 c         1695 g         6592 t
ORIGIN      
        1 tcatctatgt ttaatctgta tttatctatg tatttaaata agaatgatac taacattatt
       61 cttatgtctt tctcatcccc ttcaatatat ataccactac cttttttagt tgagatattt
      121 aggttataat tatttaatgc cactgataat ttacctattt cttctctgac aatattttta
      181 tttatgtaca acttattaga caaatcattt aaagtaactt ttttattatt acaaagtagt
      241 aaatttaata tctcaacttg cttatgaagc tcactattta ctcttttaac ttcacctcgc
      301 aacttctgat ttaccagtat tttatctgtt ccatctccac taaatttaat tccaatatta
      361 ggttttcttt tgatacaagc attacaaaaa gtagatagcc attcatcaat atatttgcaa
      421 tcatttctta tcgtcctttc tgaacactca aaatcattgg caatttcatt aacatttata
      481 aactcttctt ttaatagcaa tgttgttaat aaattttctt gtcttttatt caaacatatc
      541 acctctttat gactcaacct taatttatta tatataaaaa agagctatac ataaaggtat
      601 gctagctctc ttttaatatg atatttagct gacctggttt ttgaccccac actcgcctac
      661 tggagggttc gcttgggttc gccgcactac tactctctct cgattttatt atcggtaggt
      721 cttacttcta agccgcacca tgatcattaa cccaattttt agtgttaact tacgtggatc
      781 cttttgcctg cgactggctt ggactttcaa ccacagacct aaatatcacc tattcatttt
      841 aatattttaa attaaaattg tcaatatata agattattcc ataatataat tatcatttgc
      901 ttttgtaagt agagttaata caaaaacctt attagcaccg gataacttta aaagtttaga
      961 gatttcattt gttgttagac cagtagtaaa tatatcatct attaaaagta tatttttatt
     1021 gtttattaat ttaatatttt ctttaacaac aaatacattt tttaactctt cttttcgttc
     1081 ttttttattt aatttatata gcattcttgt atattttttt ctacttatac aatccaataa
     1141 tggaatattt actattttac ttaaattaaa tgctattttt tgcgcctgat taaaacctct
     1201 tttatttaac ctttttttat gaagtggaac aaatagtaca taatcaaatt ttatgttttc
     1261 taaatataat ttttctttca ttatttgagc tatatactta gccataaagg ttttttggtt
     1321 atacttcagc cctaacacta tcttttttgt tacatcatta tattcaatac aagatattga
     1381 tttatcaaag taaaagtctt tgttaaaaca ataactgcac tcttcaataa actctttttc
     1441 aattgaatga tgtattattg gttttccaca cttcatgcaa ccatcttgta taaaattcat
     1501 ttctttaaaa caacttttac atattgaata agtatttgtt ttctttatgg atttatcaca
     1561 aattatgcaa cttatattct caggatatat aaaatccaga catttattaa tcatattttt
     1621 aaaatcctta ataaaattat cattgcatat tttcatattt attctatcaa caacccttct
     1681 tgcttaaatt tatttagctt ataccctaaa tttgaatacc tttgattaac tctattattt
     1741 tttatcatat attcaagata cttcacatct ccaactaata caactaattt ctttgctctt
     1801 gttactgctg tatataataa attacggcta agtagcatag gaggagccca agttattggc
     1861 aatactacaa ctggaaattc acttccctga cttttatgta tcgttgtaca aaaactatgg
     1921 tcaatttcat ctaattcatc atatagatat gatacaattt tagtctggtc aaataataca
     1981 taaatagttt tcttatcttt atctatgtga taaacatatc ctatatctcc attatagata
     2041 ccttctccac tttctttttg gtcttctgtt tcccattttt ttgtataatt attttttact
     2101 tgcattacct tatcgccaac tctaaataat cttttagaaa aactttcttc aactttaaat
     2161 ttttcttttt tgttaagata cttctgtaac tctatattga gattagtaac tcctaattca
     2221 ccttttctca ttgaagataa aacttgtata tcttttaatt tatcaacttt ataaaattta
     2281 ggtaatcttt cattaaccaa tcctattatt tcatttaaaa tttcttcatt agttgatttt
     2341 cttataaaaa agaaatcttt accttttgta tttagataaa gtggttctcc attatttatc
     2401 ttatgggcat ttacaattat catactttct tgcgcttgtc taaatatttc gtttaattta
     2461 actacattta taatattaga actaatcatg tccttaagta catttccagc acctacagag
     2521 ggtaattggt cactatctcc aactaaaatt actcttgttc ctaactttat agctctaagc
     2581 aagttgtaca ttaaaataat gtctaccatt gatacctcat caacaattat tacatctgaa
     2641 tttattgggt cttcttcatc cttaaaaaaa gttaaatcat catctgttga gaatcccatt
     2701 tctagcaatc tatgtatagt ttttgcttct ttattagaag tttcactcat tctctttgct
     2761 gctcttcccg ttggagctgc caaagtcacg cttttaccat tgttttcaaa tatgtctata
     2821 atagcattga tagtagttgt tttaccagtt cctggcccac cagttataat taatactcca
     2881 ctattaacag attctttgac tgctaaaatt tgtttttctg ctaacttaat tttcttattt
     2941 tcttctaaaa cattaatctc gttatctata tctattttta agtcttcaaa ttcatactgt
     3001 gataatttta ctatttgact acatactcca ttttcagcta gatagtatgg cataagatat
     3061 atgagatttt cattgtttac tttctctata tgtatttttt gattataaac tagcatcatt
     3121 acacactctt ttattatttt cccacttagc tccaataatt tttccgaatc ttgtattaga
     3181 acatgctctg gtaaatatgt atgcccatta cttaaagatt tatttaaagt gtataggata
     3241 ccctgacaaa ttctatcctt agagttttta tctataccaa ttttatttgc tatactatca
     3301 gctactttaa atccaattcc tcttatatct tcagcaagtt ggtatgggtt tttatttata
     3361 acatctattg cactgctttt atatttttta tatattttta ggcagtaatt tggtgttatt
     3421 ccaaaaggag atagctgtat tattatattt ctcaattctc tgtcttcttc ataacttttt
     3481 actatttgtt ttactttttt actccctata ccttctactt cttgtagttt ttctggtgaa
     3541 ttttgtatca cctctaacgt atctacacca aacttatcaa ttattctctt agccattttt
     3601 tcaccaatac catgtatcat tccagaagat aagtaaacat aaattccttc taaggatgat
     3661 ggtgttactg gcataaaact attcacttca aactgagtcc cataaacctt atggtttacc
     3721 cactttcctt caacttctat gctttctcca attgccaaag taggcataca tcctacaacg
     3781 acaatctcat cattttcatt taccagatga gctattgtat agccattatc ttcgttttta
     3841 aagactattt cacttatcat tccttctaat ttttccattt ttatctccta aaaatactaa
     3901 taatcgctcc atttaaatgt tagtataatt ttatatatta aaactatgat aatcttagaa
     3961 aagttttata tttttatact taacgtaatt tccaactatt atacatttta tcagaaacaa
     4021 aaaaaatctg catccataaa atatggtacc atgaaataca ggtaccatat ttaaatatct
     4081 attttatttt aattactcta tgtttagagt ataaattaaa tttcataaga atattacagt
     4141 gctaacttat ttcagattta ttctccttgt atccctcggt tttagtaatt cttctataaa
     4201 gaatacatag taaaattaaa attcccatgt aacctgtata tggatatagt gtacctacta
     4261 acttatcaaa tggtagtaga cctcctatac aagctaatat acttactact attgttataa
     4321 ttttaaattt agggtggtca tcttctacaa atctgttagt tactgaccat aacaatggta
     4381 cagctgtagt gtatattcct agtaaaagta ctactgagaa taaaatacct attattggag
     4441 atatcttatc agctaagtaa agtgctggta tctcttttgt gtatatgttt cctatgtctg
     4501 aaagtagtgc caaattcatc attatagcag ctgccataag tgctactcca cctactatac
     4561 caccccaaac agcatctttc ttatttactg cagaagctcc cattcctgtc aagaatgtta
     4621 ttactattat tacattataa caagtataaa gtactcctga aaagtatcca tttgatgtag
     4681 cagttttaat atttaagcta tcaaccatat ttccagcatt tgataaagca tcaatattgc
     4741 taaataaact aattgctcct accaaaagtg taaatattat tattataggg cctatattac
     4801 ctagtatttt agaaagtctt gttaaaccta atgaaacagt tattagggaa acgattgcca
     4861 taccaatcct acctacatat ggatttaatc catagtactc tgataatgta gctcctgctc
     4921 ctgcaatcat tattactaat gttccaaata aaaacaatgg accaaaccat tcaaaaaata
     4981 ttccaaggta ttttccacaa tacacttgat aaatttttat tggctcctta agttttaatt
     5041 ctcttccctt atctataact tctgctccaa accatgaaaa tagtaccata gatattattc
     5101 cacctacaat accagcatat ccataaaacg taaaaaactg tagtatttct tgaccagttg
     5161 caaatccaga acctattaca gtagcaacat aagctccagc aaaacttata accgttttaa
     5221 tatttacttt acctgacata cactactccc cccaattaaa attattttaa agtgtaagtt
     5281 tattaagatt atattagact cattattatg atatttcaat atatttttaa ttatttaaca
     5341 ttagttaaca cattcttaac catcctttaa catatatctc ataatattta taaatttagt
     5401 agattattat aaaaaatgta ttgaccagat taaatactgt ttatgtatta tatgatgata
     5461 aattagcatt tattaaaaat atttaactca taataaaaag ctgttagaaa gtaattgtaa
     5521 aattacattc taacagcttt ttagttaaaa attattcacc taaagcatct tttcttaact
     5581 gctctacttt gtcagttctt tcccaaggca aatctatatc agttctacca aagtgaccat
     5641 aagcagcaac ttttttataa agaggctttc ttaaatctaa gtctcttatt atcgctcccg
     5701 gtcttaaatc aaagtgttta tttactaatt caacaagttt ttcttctgaa actttacctg
     5761 tcccaaaagt atctataaat atagataatg gtctagctat acctatagca tatgcaagtt
     5821 ctatctcaca tttatctgca agcccagatg caacaatatt ttttgcaaca tatctagcgg
     5881 cataagcagc agatctatca acttttgtag ggtcttttcc tgaaaaagct cctccaccat
     5941 gtctagaata tccaccataa gtatctatta ttatttttct tcctgtaaga cctgtatctc
     6001 cttgtggtcc tcctataacg aatcttcctg ttggatttat ataaatttta gtatcttcat
     6061 caagtaattc tgctggtata acctctttaa taacatgatt aattaaatct tctcttattt
     6121 tatcatttga tactgtttca caatgttgag ctgatataag aactgtatgt actcttacag
     6181 ctttatttcc ttcatattca acagtaactt gagtttttcc atctggtctt aaataatcaa
     6241 ctatacctgt ctttctaact tctgtaagtc ttcttgataa tttatgtgcc aaagatatcg
     6301 gaagtggcat taattctgga gtctcattac atgcaaatcc aaacattata ccttggtctc
     6361 cagctccaac tttttctatt tcctcttcta tctcttctcc agtcttactc tctagacctt
     6421 catcaactcc catagctata tctccagatt gttcatcaat agaagttata actgcacaag
     6481 tatcacaatc aaatccatat tttgctcttg tatatccaat ttctcttact gtttctctta
     6541 ctaatttagg aatgtctaca taagcagatg tacttatttc tcctgctact aaaactaaac
     6601 cagtagtaac tgtagtttca caagctaccc tagattgcgg gtctttttct aataatgcat
     6661 ctaatattga atctgatatt tggtcacata tcttatcagg atgtccttct gtaactgatt
     6721 ctgacgtaaa taaatgtctt gccattttta ttcccccttg tttattctta gtttttatta
     6781 taggttttac cttattttta attttagaaa tttactcaaa ataaaaaacc tctcctttat
     6841 aagaagaggt taataatcgt aattacatac taattaactg acctcatctt tcaagactct
     6901 tgtcttgtag gaattagcac cttttcccat ttgggtggtt gctgggcttc acagggccta
     6961 tccctcagcc tctcttgata aggtaatcaa gatttaagtt ttgttttact ttactagctt
     7021 actatttttt taaaattttg tcaatatttt tagtcatatt ttttctattc ctttttttgt
     7081 aagacaaata aaagtttcta tctatctgaa atttaaagtt aggtcttttt aatgcttttt
     7141 attcatatat ttttttgaag ggggtgttcc tattgtattt agctaaagta aattacagcg
     7201 atgatgatgt tatacagatg ctaagttcag ttctaaaaac tataattaat gaaaatacga
     7261 ttgttgtttg cataggaaca gacagggcta taggtgatac tttaggccct ttagttggaa
     7321 caatacttaa gaacagtaac tttaaatacc ccgtttacgg tacactagat aaccctattc
     7381 acgccttaaa tatatatgag tctctagata ctataaaaaa tacacatata caaggtaact
     7441 tcttggctat agatgcttgc ttggggtcgc agagtaacat aggtaatata cagattagag
     7501 aaggacctat cctgcccgga aaaggtgttg gcaaaaaact acctcaaata ggaaattatt
     7561 ctatagtagg aattgtagat aaaattgatg aaaataataa actttcattt aataatatac
     7621 gtcttagctt tatattggat ttagctgaaa caatagcctt ggctttatta gtatccacct
     7681 aatcccaata taaactaaat gataaaattc tttatataaa aaggtagata ttttagagta
     7741 tcttattaaa tactccaaat tatctacctt tttactttta tattttaagt tcttattttt
     7801 ctactaaagc aaacattaat tcacctttac agacaagttc ttcacctaca tatgccttag
     7861 catctgcctt ggctatgttt tttctaagag ttgtcatttc aacttccata gttaatgtat
     7921 ctcctggtct tacttctctc ttaattctaa ctttatctat acctgcaaat acacctaatt
     7981 ttcctttgtt ttcctctaca gacatcattg ccactccaca cacttgtgct agagcttcta
     8041 ctattagtac tcctggcatt aaaggatatt ctggaaagtg accttggaaa aatggttcat
     8101 taactgttac attctttata ccaacagccc ttttccccac ttctagctct gtaatcttgt
     8161 ctactaataa aaacggatat ctatgtggta ttaatttttt tatttcatct atatttaaca
     8221 ttctaaactc tcctaattat tttcttttga aagacccacc agtttcaagt aggtctattg
     8281 aacctaaagc tattcctgta ccttttgcaa cacaagataa tggttcatct gctattgtaa
     8341 cagttattcc tgttctctgt tctattattt tatctaatcc atataataat gaacctcctc
     8401 cagtcattat tataccaaca tcccctatat ctgctgctaa ctcaggaggc gttttttcca
     8461 aaacagcatg agtagcaact actatttgct caacacattc tctaagtgct tccaacattt
     8521 ctgttgaatt tatagttata gaactaggaa gtcctgtaat taaatttctt cctgttatct
     8581 tcatatattt ttcttcttct ctcttaaatg cagaacctat ttcaaacttg attttctctg
     8641 ctgttctttc tcctataaga agattgtgtt gttttttcat atatttaact atagcatcat
     8701 caaatctatc tccaccaacc tttatagatt cacttacaac agctcctcct agagatatta
     8761 cagctatatc tacagtacct ccaccaatat ctataatcat attaccatta ggctgtgata
     8821 tgtctacacc agctcctatg gctgctgcta ttggttcttc tattatatat acttctctag
     8881 ctccagcttg tctcgtagca tcttctactg ctcttttttc cacttctgtg acacctgttg
     8941 gtacgcatac cataattctt ggcataaaaa atcttccaaa tcccttttta tcaactatct
     9001 tatctataaa agattttaac atcttctcag ttacatcata atctgaaatt acaccatctc
     9061 ttaaaggtct tatagcgact atattacctg gtgttcttcc tatcatcttt ttagcttctt
     9121 ctcccacagc taaaacagaa tctgttcttt tgtctatcgc cacaacagat ggttcttcta
     9181 aaacgattcc cttaccacta acataaacaa gcacatttgc agtacctaaa tctatgccta
     9241 tatcagctcc agccattctt tcaactcctt tatttataat attagattat attataagcc
     9301 tattttaata ctcctataat aaagcatata caaaaatgtc gctttaatca agagaaaata
     9361 aatcttttga gagtattatt attttgctat ttataattac ttaatatatt tctttttata
     9421 atttaattaa ataaaaaaag taggttttta acctacattt ttttatgttc tttctcatat
     9481 tttaatttcg ttgctaatcc ccctctaata tgtctctctg atttattttt gtctagcaca
     9541 tttttaactt ctttggcaag ggatggattt atttccttca atctttctgt aacatcctta
     9601 tgaattgtac ttttacttac tccaaatgtc ttggcggttt gtcttactgt agtgtttttt
     9661 tcaagtatat attttgctac cactatggcc ctttcctcta tatgagatct caaagattac
     9721 ccccctcaca taatagcttt aatgatatat atgtaaaaac accaaaaaat ataacaagct
     9781 taaccaaata tgattaaact tgttatactt ttacttaatt agactcattg ggtctataga
     9841 tttttctcca ctatatgcct ctaaatgcac atgtatacct tcttcacttt ctatttgaga
     9901 agtactacct acagtgccta gagattgtcc ttctgttatc ttctgttctt tcttaacatt
     9961 aacattttta tctagatttg aatatactac tacaatgtta ttatcacttt ttatcttgac
    10021 tgattgtcca tattcttcat cattaaatac atctactact attccgttta atagagattt
    10081 tacttctttg cctttagtac aactaatatc tacaccttta tgagtttccc aaacatcaag
    10141 tgttttagaa taactaggtt ctttttctga atatcctctt attactttat ctccaatgta
    10201 atttaatttt gttgtactcg atttattttc ctcttttgat ttagcttttt ctaagttttg
    10261 ttttgaatca gttgcagtag ggatagcatc tttcttgtct ttttcaatta aatgtatttc
    10321 ctcttcacta tctttattag cattttccat tattccttta ttagaagcca atttatctac
    10381 attattattt gtaaaccaaa ctccacctac tgctaataaa caaacacata caaacaaaga
    10441 taaataaaaa ccatcttttt ctaacagctt tttcttcatt ttgcacctcc aaatatattt
    10501 ctacatttgg attattgacg tgtttttggc attttataca attattagta tatatctttt
    10561 atttttgtat ctgtgtagta atgtgataat atatcataat atttatagcc ctcttctgcc
    10621 attccttctg ctccccactg gctcatacca acaccatgac catatccttt tactacaaag
    10681 tctatgtatc cttctccaaa ttttatgtca aagtttgcag agtttaactt aaaaacagtt
    10741 ctaatgtctt ttccagtcaa ttctttattt cctaatttta ttttttccac agttccagca
    10801 tcacttcttt tagttattga gacttgttta cttaaattat tcacatctat aacaatagtg
    10861 ctatatgccc ttctaagact cttaacaaaa tctgtgttac ttattttaag tgttgatgca
    10921 aatttagggg aatacttatc ataaggactt tccacagatt ttaaataagg atattttgca
    10981 gaaaatactt cttcactatt ttctgttttt cctgaagatg tagaaaaata aagaggaagt
    11041 attgctttat cattataagt tattatttga cctttagttc ctctaactgc ttcttggatt
    11101 ttggaatatt tattcttaat ccattcttca ccatttaatt ttttaagagt atcataactc
    11161 ttatattctt gacaatgttt ataatcagta catactactg catttttatg tttacttgat
    11221 ttaccatgtt cctgtttata tactacatat gttctagctg ctacagattg agcttttaaa
    11281 gcctctatat caaactctga agacatctca ccagctaata ctccacataa ataattctcc
    11341 atgtccattt tttcggtttt tcctgttata tgattatata cattgatgat tggtgctttt
    11401 ttatttacag tttcatagtt cactatgttc ttttcttctt tattactttt gtctttaata
    11461 tcactttttt taatagtttt gtttatagat actgggctct ctggtttttc agttaattct
    11521 acatttttat aagaaactaa agttataagt gatggaacta acacagagca agttacaaat
    11581 cctagcaaaa caatcaatgg gttcttcata tactctcctc cttagctttt aaaaccttgt
    11641 atttaagtat atgaacaacc ctattaattt ataactttct tctataaacc aattattaat
    11701 cttctactat ttcgatttga ccacctaatg ccgttatctt cttatctata tcaacatagc
    11761 ctctttggat atggtatatt tctcctattt gagtttctcc ttctgctata agaccacata
    11821 aaataagggc tgcccctgct cttaaatcag tagcgtttac tgctgcacca tgtagctcat
    11881 ctacaccatt aacaaccgca cttcttccat ctatttttat attagcaccc attctattaa
    11941 attcagcaac atgcataaat ctattttcaa aaacagtttc tataactaca ccagttccat
    12001 tagctacagt aagcatagcc ataaattgtg cttgaacatc tgtaggaaat cctggatgtg
    12061 gtaaagtttt tatatctatt ggttttaata cctttggacc tacaactctt acagaattgt
    12121 ccatttctgt tatttcacaa ccagcttctc tcaacttagc tacaactggt tttaaatgct
    12181 ccattaaaac attttcaaca gttatatcac cttttgtcat ggctgctgca accatataag
    12241 tcgctgcttc tattctgtct ggaattacat tatgttctgc tccttttagc tctttaacac
    12301 cttttatttt tatagtatta gtacctgctc ctttaacatc tgctcccatt tcattcaaga
    12361 agtttgctaa atctactatt tctggctctt ctgctgcatt ttctattatt gtagttcctt
    12421 ctgccaaaga agctgccatc attatatttt cagttgctcc aacagatgga aaatctaaat
    12481 ataatttatt tcctactaat ttttctgttg ctgcttcaac aaaaccatgg tccatctcta
    12541 tttttgcacc taaagcttta aatcctttta aatgtaaatc tataggtctt gttcctatag
    12601 cacatcctcc tggcatagaa attttcgtac tattaaatct agcaagtaat ggccccataa
    12661 ccaaaaaaga agctctcatc tttcttacaa gctcatatgg agcttcacaa gtttttatat
    12721 tgcttgcatc aacagtaagt gtattttctt tatattcaac ttctgcccct acatgtctta
    12781 ataaatctga tataacatga acatctctta aatttggtac tccatttaat gttgatttcc
    12841 cgtttgctaa taaagtcgct gctattattg gtaaaactgc attttttgca ccatctattt
    12901 taacactacc tttaagtgga ttactctttt ttactattat cttagccata ttttttgatc
    12961 tcctcataat taataatcta attttataat tggtgtggca atatatatga gtgttcttcc
    13021 gtcgtcctca ttatatttaa ttcctatatt taaattaatt ttttccccta aatattctaa
    13081 ttcataatca tttaatactt tactatatgc tgttatagat aaaaagttta cgtctttcac
    13141 ttcatctatt tgcttagcac tcatattata taatattttt tgtaaaatat catcatattt
    13201 gttgttttgt aacctttttg tgtattctcc aacaatacaa gcagacacat ctacttgaga
    13261 tgaatattta tttaacacat tttctacgtt actataaata acctctttat gtttatatac
    13321 tttgttatcc aatatgtcaa ctactatgta actttctttg ttatttttat ttattccttt
    13381 tatagaaatt tctttatcgc taccttttat agtagcacat aattgtattt tatcgccaga
    13441 cccttttcga ttccaattta ttcctttttt atccaacttt agattgttaa ttatttctat
    13501 aaaaatatta taaaattctt tatttgataa attatgattt ataacagaat taattttaat
    13561 attataaaat ttaaaatttg cttctgaaat ttcaagagct ttgcttaatc tattgtatct
    13621 agtatttata ctagtatctg cataagatat tgtcattcct ataatcatta atataaaaaa
    13681 gcttatagct aattttactc ttttcatagt aaaaaatccc ccttaaacat gtttttctgt
    13741 ataatagaat tattaacaaa tttaagagag atatactttc tcaatatgta tttttataca
    13801 tctttgtatt tttcatctaa taaggctctt tcaaactctt caatctcaat tttctctatc
    13861 cttcttctgg atttgccaat tttttatatg tatcaaatac atcttgcact agttcttata
    13921 tattctcttc tgtaatataa attacttagt ctatacctaa atctataatc tataatctat
    13981 aatctataat ctattcacat tttaccagca aaataaacag ataatacttt tttatcaatt
    14041 tccatagctc caaataaaca tacctaagta cacttcccaa aacacaattt acatactctt
    14101 cctaataaat caaaaacacc tttgttttaa ataactctat cgtactttca aaaactttat
    14161 ttgtgttctc ccacttcaat taaatttgaa ttatcaagac tccagcccat ccatccatca
    14221 ctataaagtg aggtattttc cactcctatg acatttgcgt atgttaatac ttcagcagca
    14281 cgccatccac ttccacacat aaatataagc tgtttattca catctatatt ttccttgtcc
    14341 cacatttcta gaatctcata tttatttctc attgtattat caatattacg atattcttct
    14401 agtgaaacac tatcactacc tgaatgtcca tataaagctc ctggtatacg ccctttttta
    14461 tcataatatg tatatccaga tacttttcct atatattcat cccaagtacg gttatctact
    14521 agtacaaact tatttttttc ttttagtttt tgtcttaatt cagatgtagt aacaactaat
    14581 tgtgagttaa ctggtatatc tgctccaaaa ttagtacatg aatgttttgg attactagta
    14641 aattctaatt catatcctgc tgataaccat gagtttgttc cgccattcaa aactcttaca
    14701 tcttttactc ctatatatct caatataaca gctagtctat aagatgccat aggtgtacta
    14761 cttgaaacta ttacagtatc atcctttgtg aatccatagt ctaaagcaaa cttagtcaag
    14821 ttatcatcat tatcaagcat ccatgttgga ggaggttcta taatatcggt atttacatga
    14881 acacttgtag gtatatgccc ttttgtatac gattcttctc cccaactcgc ttcaatcatt
    14941 tttatattct tggaattttc aaatgtctca ggaatttttc catccaatat atcttttatt
    15001 ataaaagcag gaactatcat ttgataattt ttatatcttt ccattggaag attttcgttg
    15061 tctgcccatt gtttaatatc atatttatat agatatttat atcctttttt agataaataa
    15121 tctgctacaa ccaaagcatc ttttccattt gcatcatata atactatatt tttatctata
    15181 tcaattcttt tagttttcaa tgcttgttct agcacttcat cttttttatc tgagtataca
    15241 cttaaccaat tagcagaaaa atctactgct ccttttatat gtccacctct ttttactcca
    15301 tctaacttcc agccattata agcatcattt aagcgagtat caactataac ccaagcgtta
    15361 tcttgcactt tttcttgtaa aacttcagta gatattgcct taagattaac aaaataatct
    15421 tctttctttt cttttttaaa atcatttgcc ataatttata ctcccaattt aaaaattttt
    15481 tatttaaaat cattttagta aaataaagta attttcttat actctattag cttcattata
    15541 ttcttttata aatagttcct tatattttta ttttaaacta ctcactttat tttatcacaa
    15601 ggttactaat accttatgta ttttaatctt tctcataatc tagtattttt tatgtcttat
    15661 attgactatc tgtttattaa gtattcacta tatctgatat cgactatatg tttattaagt
    15721 actcactata ctttatatga actatatatt tattattctc tataccttat atggtctata
    15781 tatttatcat tttacagatg atttaattca atctttttat aaaatttatt atagcaaaaa
    15841 caaaacaaat ttaatattta tctaaaaaac tcatcctata aaatacaaat aacaggatga
    15901 gtttttttca ataaatatta tctatttaat ttttaatcta tttttattca actgttactg
    15961 atttagctag atttcttggc ttatctacat cgcatcctct taatacagag acatgataag
    16021 caagtaattg cattggtaca actgctaata ttggtgataa aatatcatct gaatttggta
    16081 tatagattat tttatcagca gctttctcaa catccttatt atggctttga gctattgcta
    16141 ctacataagc tcctcttgct cttacttctt ccatattaga aaccattttt tcaaataact
    16201 tttcttgagt agctatggca attacaggag ttcctttttc tattaa
//
LOCUS       DAFNNB010000085        16222 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0086, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000085 DAFNNB010000000
VERSION     DAFNNB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0086"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            43..726
                     /gene="pgeF"
                     /locus_tag="L3M60_002258"
     CDS             43..726
                     /gene="pgeF"
                     /locus_tag="L3M60_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HBN6226918.1"
                     /translation="MDEKLKSVNLVITCKNIDAKNREDMLKVCKECDLTFENLTSNSQ
                     IHSDIVNIVNKDTIGKRRDGDALITNLEKVPLLLFTADCVPISIIDAKNKAIGLAHAG
                     WRGTFSNIGHKTIKLMSECYKTVPEDLVCIIGPSIGPCCYEVSEDLVEKFNTILTNND
                     EKFYIIKEGSYYLDLWKINEYMLICSGVKKENIINLNLCTSCRADKFHSYRKHNKASE
                     RIGTVLQIK"
     gene            741..1433
                     /locus_tag="L3M60_002259"
     CDS             741..1433
                     /locus_tag="L3M60_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226919.1"
                     /translation="MNTKVLVIDDEMHIVELLKFNLEVSNYEVSYSYDGFDGFIKAKE
                     IKPDLILLDWMLPNISGIEVLRKIRSDKDLKNIPVIMLTAKNMENDKVEGLEIGADDY
                     ITKPFSIKELLARISSVLRRYNLTSLGEENNILTTGNLKLDLSKHEVTKGSEKIELTL
                     KEFELLKLLIQNKGKVLSRNYLLDKIWGYEYYGETRTVDVHIRYLRKKIEDEDKSEKY
                     IETIRGVGYKID"
     gene            1462..2751
                     /locus_tag="L3M60_002260"
     CDS             1462..2751
                     /locus_tag="L3M60_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6226920.1"
                     /translation="MEDLNSVYFFIVMLSIAIVILAVRYTITLRKYLKEFIYVSKKVS
                     NNEYHARLNISAKGELGELARNFNHMIKNMDKTLEEVQYKHLQLKSILKSISHGILAI
                     DVNGNILLINDEAKEMIKSEPNQLVEGHNINVVIKEEEILKQILRFIGSKDNETANIT
                     TKDDIVYKIKVDPVYLQNTNNVIIGSIVNIEDITKKVKLENMRSDFVANVSHELKTPL
                     TSISGFVETLKSNENIDVNTRNRFLEIIESESDRLKRLIDDILLLSFVENKDTLLMEN
                     VVVYDVFKEVYEITQHSASEKNIEVLYKCDDKSISILSNKDYIKQVFLNLVDNAIKYT
                     QDNGIVQVVVLKDEEKIVIKVIDNGVGIPKEDVERIFERFYRVDKARSRDVGGTGLGL
                     AITKHIVKSLNGCIKVKSELGIGSEFIVTILQKNFLK"
     gene            2806..3738
                     /locus_tag="L3M60_002261"
     CDS             2806..3738
                     /locus_tag="L3M60_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBN6226921.1"
                     /translation="MEESLLNANLFRHSFIVALVVGILCRGLVLRVTDKQYPSRPQDY
                     LEQIIISGLSASLGAIALPALIDKEFAALTFFAVAIQQFQGLAEQERITLKNIDNEEL
                     VPKGDAYVEEIASTYESRSYISLFSALVSSIVYIVFARKYDLSFFYCTILAIVSGAIV
                     GLIFRRFLRRNSIADIADIVPAKISFEGPILMVNDVIITNIGLESTREKYRNECLAIE
                     VIPRDLGAFGIVNDIGQRQAIIHNLFIHMGIDRDVDEVDIVAISKTNLEKRTVVIPYM
                     PILKDIDVMIDVVKSTPIIETSKGKQSDFSRKTL"
     gene            3784..4716
                     /locus_tag="L3M60_002262"
     CDS             3784..4716
                     /locus_tag="L3M60_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HBN6226922.1"
                     /translation="MDRVFFVAVIIGITSRIIMLNLDQKQYPTQPNVLISQLILAFVA
                     SSLGALLVPALINRSYTSITFLSLAAEQFRQVRANRRNTLQNLEEVQLVKRGAAFIEE
                     IARTYEVRNYMCIVTSFLTIGLYYVILAETKISEGMSLVISSICGLLLAFILKKLLTR
                     KSIGDIADVMPAEISFVDESILKVGDLKGITNIGLKSDREVYINRGVGIQIIPKNKDY
                     ANAGILFDSGQRQAIVYNIYSRLGLYGDYGEPSFVPLPRRNKEDESIMIAYLPVDKNV
                     EKVMEAVKSCPILSSSKGKNLSLKNYKIGKKGSA"
     gene            4718..4918
                     /locus_tag="L3M60_002263"
     CDS             4718..4918
                     /locus_tag="L3M60_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226923.1"
                     /translation="MGMDIGLNEYTLAIVTLDKNMKNGGGCPIFYANDEKDLQNKALL
                     MSKCVGGMVHDMTNGTLIIVKH"
     gene            5154..6482
                     /locus_tag="L3M60_002264"
     CDS             5154..6482
                     /locus_tag="L3M60_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HBN6226924.1"
                     /translation="MEVNVKKVNNIISKVYKDSIAEEIGIEVGDLLISVNEQPIHDII
                     EYRFLLSDEYLEVEIQKKDGEVYIYEIEKDYDEDLGVEFTNPIIDQAKSCRNKCMFCF
                     IDQLPEGMRETLYFKDDDSRLSFLQGNFVTLTNMSEEDINNIIKYRISPINISVHTTN
                     PELRQKMISNKFAGKLYGIMKRLADAHIEMNCQIVLCPGINDGKELDRTIKELAQLYP
                     YVNSVAIVPVGITKHRENLVELNIFNGKSASKTIEQIHQMQQKYLKQLGTRFAFLSDE
                     FYILSNSELPGYEEYEGFLQFEDGVGMIRKLKTEIEEYLNILPEDILKRDKKVSIATG
                     HSAYEFIQSMANAMMDKFKNLQINVYEIKNKFFGETITVSGLLTAKDLKEQLENKELG
                     EALYITRSMLKADEEIFLDNIELNQLEELMKIKIIPCLNEGKDFVDKILK"
     gene            6516..7835
                     /gene="der"
                     /locus_tag="L3M60_002265"
     CDS             6516..7835
                     /gene="der"
                     /locus_tag="L3M60_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBN6226925.1"
                     /translation="MSRPVVAVVGRPNVGKSTIFNKFAGKRISIVENTPGVTRDRIFA
                     EVEWLDKYFTLVDTGGIEPDSEDIILSQMRNQAMLAMDMSHVILFIVDGKAGITAADK
                     EIAQLLRKTKKPVILVVNKIDSQSQFDNIYDFYELGFGTPFAVSGANSMGFGDLLDEI
                     VENFPAGLDTEYEEDIIRVAITGKPNAGKSSILNKILGEERVIVSPIAGTTRDAIDTY
                     FEKNGQKFLLIDTAGLRRKSKIYETIEKYSVIRAMSAVDRADVVLIVIDALEGVTEQD
                     TKVAGIAHDEGKGCIFVINKWDLIEKDNKTMSNYTKDIKEKFPFMMYAPIVFVSAKTN
                     QRMNKILDTVEYVSNEHSKRISTSALNEVIGEAVMLNQPPSDKGRRLKIYYGTQTDIR
                     PPKITLFINDKELTHFSYQRYLENKIRENFGFEGTSIKFEYRQKNKK"
     gene            7869..8483
                     /gene="plsY"
                     /locus_tag="L3M60_002266"
     CDS             7869..8483
                     /gene="plsY"
                     /locus_tag="L3M60_002266"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBN6226926.1"
                     /translation="MEIFSYIIIAVVAYLLGNISTSYIVAKRIAGVDIRTQGSGNAGS
                     TNVLRTLGKRAGAMTFLGDVLKGVMAVLISEFAARLVGIDTLLAGYLAVICVVAGHNW
                     PAVLGFRGGKGVATSLGAMLAVNPVITLMCLAVFILVVVITKYVSLGSVVGIGCSPIF
                     MIMVKNKAGLIVALFLTASVIYNHRANIKRLLNGTERKIGQKKE"
     gene            8514..9533
                     /locus_tag="L3M60_002267"
     CDS             8514..9533
                     /locus_tag="L3M60_002267"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBN6226927.1"
                     /translation="MEKVCVLGTGSWGSALALGLAKKGNDVSMWTRKEEQAKKINRTK
                     ENTDYLPGVLFPNNITISTDIEKTIKDCKVIILAVPSQAVRSTCQKIKPFIKEGQVIV
                     NVAKGLEKGTWLRLSQVCEEELPQNPYVILSGPSHAEEVARDIPTTVVVASKDLKIAQ
                     MIQDLFMSPKLRVYTNPDIVGVELGGALKNIIAFGAGICDGLGYGDNAKAALMTRGIS
                     EMSRLGIAMGANMSTFAGLSGIGDLIVTCTSMHSRNRRAGILIGKGMSLEDTLKEVKM
                     VVEGITATEVAHDVAEKLDIDMPITNAIYSVIKKGSNPKEVGIELMMRSKKHEMEEVV
                     LGDDI"
     gene            complement(9689..9961)
                     /locus_tag="L3M60_002268"
     CDS             complement(9689..9961)
                     /locus_tag="L3M60_002268"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226928.1"
                     /translation="MFPEDLGVHDSDISLMITGESIKERNKILLNNLVDTGYIPAKDL
                     DIINDIIISFYQNLLFLKKNTGKNSDDDELTMKTISSIEYIIKHAK"
     gene            9971..10153
                     /locus_tag="L3M60_002269"
     CDS             9971..10153
                     /locus_tag="L3M60_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I V"
                     /protein_id="HBN6226929.1"
                     /translation="MFCYINPKGTTVGIEKHIVSASFSEYMTDKSFVIKDVGSKIFEM
                     RYVKDVGDNALLKYVS"
     gene            10166..10342
                     /locus_tag="L3M60_002270"
     CDS             10166..10342
                     /locus_tag="L3M60_002270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226930.1"
                     /translation="MYIKFFNCICSGIDRTAQLHLDSNTRILQRGDIVIHSRQVWYEN
                     YRAENERISICKRN"
     gene            10432..11907
                     /gene="spoIVA"
                     /locus_tag="L3M60_002271"
     CDS             10432..11907
                     /gene="spoIVA"
                     /locus_tag="L3M60_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HBN6226931.1"
                     /translation="MNNNIYEDISKRTQGDIYIGVVGPVRTGKSTFIRKFMEKLVIPN
                     IDNEFKKDRTRDEIPQSGSGKTIMTVEPKFVPADGVEIKIKDTVSLKVRMVDCVGYIV
                     EGALGHEEGGKQRLVSTPWSQEAMTFEKAAEIGTKKVIKDHSTIGIVVLTDGSVTGID
                     RKSYVEPEERVIQELKNLKKPFAVVLNTLSPKSEETSMLRSELEEKYEVPVLPMNVVE
                     MEEEDIEEVMEAVLYDFPLTEIRINLPKWVEGLERNHWIKSSIITTLKQSIIDIGKIR
                     DIEGIMQGFSELEFLEDTGVDNVELGEGVINIDLQTKQDLFYNVLEEKSGFKIEGDYQ
                     LLSLITRLSKVKNEYDKIESALIDAKIKGYGVVAPSLEELSLEEPEIMKQGKQYGIKL
                     KANAPSLHIIKADISTEVSPIVGNQNQGEEMIKYLMEVFEEQPADLWESNMFGKSLHD
                     LVKEQLQSKLYTMPEEIRVKMQKTLQKIVNEGSSNIITILL"
     gene            12090..14051
                     /locus_tag="L3M60_002272"
     CDS             12090..14051
                     /locus_tag="L3M60_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226932.1"
                     /translation="MKKYEIFIFISIVVLCFISFPYKVFGQEENCLEDFERWVQENKN
                     NKEKIVYTLSCDMVIDEEFRFYIPYDSNFTIDTNKYKILIKNHGRFIIDDNELNIIGE
                     GGKEGVIHVENGGSISIGINNIIATDGTALYVEEGGDLYIKSSYESVEKIKANGKDAI
                     GIYTENDIRIINKDIEVTGERAIGVYSKGDVEIEESSIKAYSNNNENELLRDNNKLVQ
                     SIISESKKVYIIDSYNELIPSIAEDSGYNIVKCCYRGIGVFSEEIKVSKDDKIENIKF
                     PQYMVLETSSGNQLDIDVEWDFTDYYEKLEKGEKFNITGKFKTETLNKEKIIINDDVV
                     PILNIYVMDKKPIDNLELEFQNTKKGYVAALFYDMPYSASKVFVEYSSDGINWISEEQ
                     EDIRDQAILFFEDFKLRCFRIRVVGGLKEGYSNIVFKPGFIMGGGDNQETPDDEQEND
                     DIDGDRGGGGRDDPDRDKDDNSQNGNNGNDNNDSNNNQNNNNENDNSDEKQPNAPKDD
                     VESSTGSNENKNPQGDIPKEGNIPKDGNKENNSDKEKGVFNKSSIIENDGDYDLNLKS
                     IFGINEHSFPYRESISNKNQDTNKNKSNISDYNGIINNNYKLESKEYNLKKDIIFLAC
                     ILTPIMIGSILIVNSDTRKSIIKFIKEKK"
     gene            14341..14610
                     /locus_tag="L3M60_002273"
     CDS             14341..14610
                     /locus_tag="L3M60_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBN6226933.1"
                     /translation="MKAILTVIGKDKPGIVAGISDELYKQNINIVDISQKILQDYFTM
                     IMIVDLEKSLNSFEKTVEDLVERGKKLSVDVKIQREEIFNSMHNL"
     gene            14636..15991
                     /locus_tag="L3M60_002274"
     CDS             14636..15991
                     /locus_tag="L3M60_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009170073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HBN6226934.1"
                     /translation="MNVGNILETIKMIDEEQLDIRTITMGISLLDCIDPDGDKAREKI
                     YNKIMSSAKDLVKVGKDIEREFGIPIVNKRVSVTPISIIAGATDESDYVKFAQTLDKA
                     AEDLGIDFIGGFSALVQKGYTKGDKILIKSIPKALASTNKVCASVNVGSTRCGINMDA
                     VREMGEIIKETAELTKDAKGFGCAKLVVFCNAVEDNPFMAGAFHGVGEADRIINVGVS
                     GPGVVKRALEKVKGEPFDVVSETVKKTAFKITRVGQLVAKEASSRLNVPFGIIDLSLA
                     PTPAIGDSVANILEEMGLEVVGTHGTTAALALLNDAVKKGGVMACSHVGGLSGAFIPV
                     SEDAGMIDAVIKGALSIDKLEAMTAICSVGLDMIAVPGNTTAGTLGAMIADEAAIGMI
                     NNKTTAVRIIPAPGCDVGDMVEFGGLLGRAPVMPINTNSSELFTQRGGRIPAPIHSFK
                     N"
BASE COUNT         6533 a         1641 c         3096 g         4952 t
ORIGIN      
        1 gatattaaag agtatgttaa tttaagtggt aattgtaaag aaattgatga aaagttaaaa
       61 tctgtaaatc tagttataac atgtaagaat atagatgcaa aaaatagaga agatatgtta
      121 aaagtatgta aagaatgtga cttgacattt gaaaatttaa ctagtaattc tcaaatacat
      181 tcagatatag ttaacatagt taataaagac accataggta aaagaagaga tggggatgca
      241 cttataacaa acttggaaaa agtaccactt ttactgttta cagcagactg tgtacctata
      301 tcaattatag atgctaaaaa taaagccata ggactggctc atgcaggttg gagaggtact
      361 tttagtaata taggtcataa aacaataaaa ttaatgtctg aatgctataa aacagttccc
      421 gaagatttag tatgtataat tgggccatca attggacctt gttgttatga agtgtctgaa
      481 gatttagtag aaaaatttaa cacaattctt acaaataatg atgaaaagtt ctatataata
      541 aaagagggga gttactattt agatttatgg aaaattaatg agtatatgct tatatgtagt
      601 ggagttaaaa aagaaaatat aataaattta aatttatgca ctagttgtag agcagataaa
      661 ttccactctt atagaaaaca taataaggca tcagaacgaa taggaacggt actacagata
      721 aaatagagag gagcttttta atgaatacta aagtgcttgt tatagatgat gaaatgcata
      781 tagttgagct attaaaattc aatttggagg tgtcaaatta cgaagttagt tattcatatg
      841 atggatttga tggatttata aaggccaagg aaattaaacc agatttaata cttttagatt
      901 ggatgttacc aaatataagt ggaatagaag ttttgagaaa aataagaagt gataaggact
      961 taaaaaatat tcctgttata atgcttactg ctaaaaatat ggaaaatgac aaggttgaag
     1021 ggcttgaaat aggtgctgac gactatataa ctaagccatt tagtataaaa gagttgttag
     1081 ctaggattag ttctgtttta aggagatata atttgactag cttaggtgaa gaaaacaata
     1141 tacttactac aggtaattta aaactagatt tatcaaaaca tgaagttacc aaaggttctg
     1201 aaaagataga gttaactctt aaggaatttg aattattaaa actattgatt caaaataaag
     1261 gaaaagtact ttcaagaaat tatcttttgg ataaaatatg gggatatgaa tactatggag
     1321 aaactagaac agtagatgtt catataagat atttaagaaa aaaaatagaa gatgaagata
     1381 aatcagaaaa atatatagag actataagag gtgtaggata caaaatagat taaaaaggtt
     1441 atattttagg agtgttatct tgtggaagat ttaaatagtg tatatttttt tattgttatg
     1501 ttgtccatag ctattgtgat attagctgta agatacacaa taacattaag gaaatattta
     1561 aaagaattta tatacgtctc aaaaaaagta agtaataatg aatatcatgc taggcttaat
     1621 atatcagcta aaggagaact cggtgaatta gctagaaatt ttaatcatat gataaagaat
     1681 atggataaga cattagaaga agtgcagtat aagcatttac agttaaagtc tatattaaag
     1741 agtatatctc atggaatttt ggctatagat gtaaatggca atatacttct gataaatgat
     1801 gaagcaaaag aaatgataaa gagtgaacca aatcaattag ttgaaggtca taatattaat
     1861 gttgtaataa aagaagaaga aatattaaaa caaatacttc gatttattgg ctctaaagat
     1921 aatgaaactg ccaatattac tactaaagat gatatagtat ataaaataaa agtagaccca
     1981 gtttatcttc aaaatactaa caacgtaata ataggttcga tagtaaatat agaagatatt
     2041 actaaaaaag ttaaacttga aaatatgaga agtgattttg ttgcaaatgt aagtcatgaa
     2101 cttaaaacac cacttacatc tataagtgga tttgtcgaaa cactaaagtc aaatgaaaat
     2161 atagatgtga atactagaaa tagattctta gaaataatag aaagcgaatc agatagatta
     2221 aaaagattga ttgatgatat attgctgtta tcgtttgtag aaaataaaga tactcttctt
     2281 atggaaaatg ttgtagtgta tgatgtattt aaagaagtat atgaaataac tcaacattca
     2341 gctagtgaaa aaaatataga agttttatat aaatgtgatg ataaatcaat aagtatacta
     2401 tctaataaag actatataaa gcaagtgttc ttaaatctag ttgataatgc tataaagtat
     2461 acccaagata atgggattgt acaagttgta gtgttaaaag atgaagaaaa aatagtaatt
     2521 aaagttatag acaatggtgt aggtatacca aaagaagatg tagaacgcat atttgaacga
     2581 ttttataggg tagataaagc aagaagtagg gatgtaggag gaacgggttt aggattggct
     2641 ataacaaagc atatagttaa atcactaaat ggatgtataa aagtaaaaag tgaacttggc
     2701 attggtagcg aatttatagt tacaatatta caaaaaaatt tccttaaata gaaggaaatt
     2761 ttttttgttt tttgcacaaa ctatataaga gaaggagctg agaaaatgga agaaagttta
     2821 ttaaatgcaa atttatttag acactctttt atagttgcat tagtcgtagg aatattatgt
     2881 agaggactag ttttaagagt tacagacaaa caatacccat caagaccaca agattattta
     2941 gaacagataa ttatatctgg gctttcagct tcattaggtg ctatagcatt gcctgcactt
     3001 atagataagg agtttgcagc cttgacattt tttgcagtag caatacagca gtttcaaggt
     3061 cttgcggaac aagaaagaat aacactaaag aatatcgata atgaagaatt agtaccaaaa
     3121 ggagatgctt acgttgaaga aatagcatct acgtatgaat ctagaagtta tataagtctt
     3181 ttttctgcac tagtatcttc gatagtttat atcgtttttg caagaaagta cgaccttagc
     3241 tttttttatt gtacaatctt agctattgtg tcaggagcta ttgttggttt gatttttaga
     3301 agatttttaa gaagaaacag tattgcagat atagcagata tagttcctgc aaaaataagc
     3361 tttgaaggtc ctattttaat ggtaaatgat gtaataataa ccaatatagg tctagaaagt
     3421 acgagagaaa agtatagaaa tgaatgtctg gcgatagaag ttataccaag agatttaggt
     3481 gcatttggaa tagtaaacga tatagggcaa agacaagcta taatacataa tctatttatt
     3541 catatgggga tagatagaga tgtagatgag gttgatattg ttgctatatc taagaccaat
     3601 ttggaaaaac gtactgtggt tataccgtat atgccaatac tcaaagatat agacgtcatg
     3661 atagatgtag tgaaaagtac acctatcata gagacatcaa aaggaaagca gagtgatttt
     3721 tctagaaaga ctttgtagat ttacatgtaa aattttaaac taaaatattt taggagtgat
     3781 ttaatggata gagtattttt tgttgctgtt attattggta taacatctag aattataatg
     3841 ttgaatttag accaaaaaca gtatccaacg caaccaaatg ttttaatatc tcaacttata
     3901 ttagcattcg tcgcttcttc attaggagca ttattggttc cagctcttat aaatcgttct
     3961 tatacatcta taacattttt atctctagca gcagaacaat ttagacaagt gagagcaaat
     4021 aggagaaata cgttgcaaaa tttagaagaa gttcagcttg taaagagagg tgctgcattc
     4081 atagaagaga tagctagaac ctatgaagtg agaaattata tgtgcatagt tacctctttt
     4141 ttaacaatag ggttgtacta tgtaatactt gcagaaacga aaataagtga agggatgagt
     4201 ttggtaataa gttcaatttg tggattattg ctagctttta tattaaaaaa actccttact
     4261 agaaaaagta ttggagacat agctgatgta atgcctgctg agattagttt tgtggacgag
     4321 tctatactaa aggttggaga tttaaaaggt ataacaaata taggtctaaa aagtgataga
     4381 gaagtatata ttaatagagg tgtaggaata caaataattc ctaagaataa agattatgct
     4441 aatgcaggaa tattatttga ttctggtcaa agacaagcaa tagtatacaa tatatattcg
     4501 aggttaggtt tatatggaga ttatggagaa ccttcatttg taccattacc tagaagaaat
     4561 aaagaggatg aaagtataat gattgcttat ttaccagtag ataagaatgt agaaaaagta
     4621 atggaagctg taaagtcatg cccaatactt tcaagctcta aaggaaaaaa cttatcacta
     4681 aaaaattata aaataggaaa gaaaggaagt gcataagatg gggatggata taggtttaaa
     4741 tgaatatacc ctagctatag ttacacttga taagaatatg aaaaatggtg gaggttgtcc
     4801 aatattttat gcaaacgatg agaaagactt acaaaataag gcattgttga tgtcaaagtg
     4861 tgtaggtgga atggttcatg atatgacgaa tgggacgcta atcatagtaa aacattaaat
     4921 ttataaattt tttaagaaca aataatattc aaatttttaa aaaacttaaa tttgaatatt
     4981 atttgtttta atctagttta tagctatcta cttatattga ctattagaat taaaatgtgt
     5041 tattatgaca tgaaatgctt aaaaatgcta ccataaaata taataaataa gtaaactata
     5101 tataaaaata tattataata agcattgatg aatacaaaag agtggtgaaa atattggaag
     5161 taaatgtaaa aaaagtaaat aatattataa gtaaggttta caaagatagt attgcagaag
     5221 aaataggtat agaggtagga gatttactta tatctgtgaa tgaacaacca atacatgata
     5281 taatagagta tagattctta ctaagtgatg aatatttaga ggtagaaata caaaaaaaag
     5341 atggtgaagt ctacatatac gagatagaaa aagactatga tgaagatttg ggtgttgaat
     5401 ttacaaatcc aataattgac caagctaaaa gctgtagaaa taagtgtatg ttttgtttta
     5461 tagaccaatt gccagaaggt atgagagaaa ctctttattt taaagatgat gattcaagat
     5521 tgtctttttt acaaggaaat tttgtcacac ttacaaatat gagtgaagaa gatataaata
     5581 atattataaa atatagaata agtcctataa atatatctgt gcatacaact aatccagagt
     5641 taagacaaaa aatgataagt aataaatttg caggaaaatt atatggtata atgaagagat
     5701 tggcagatgc tcatatagaa atgaactgtc aaattgtttt atgtccaggc ataaatgatg
     5761 gaaaggaatt ggatagaaca ataaaagaac tagctcaact ttatccgtat gtaaatagtg
     5821 ttgctatagt tccagttggg attacaaagc atagagaaaa tctagtagaa ttaaatatat
     5881 ttaatggtaa gagcgcaagt aaaactatag agcaaataca tcaaatgcaa cagaaatatc
     5941 ttaaacaatt aggtactcga tttgcatttt tatcagatga attttatata ctatctaata
     6001 gtgaattgcc tggatatgaa gaatatgaag gttttttaca gtttgaagat ggtgtaggta
     6061 tgatacgtaa gttaaaaaca gagattgaag agtatctaaa tatattgcct gaggatattt
     6121 taaaaagaga taagaaagta tcaatagcta ctggtcattc tgcatatgaa tttatacaga
     6181 gtatggcaaa tgctatgatg gacaagttta aaaatcttca aataaatgtc tatgaaatta
     6241 aaaataaatt ttttggagag acaataactg tatctggtct tttaacagct aaagatttaa
     6301 aagaacaact tgaaaataaa gaattaggag aagctcttta tataacaaga agtatgctaa
     6361 aagcagatga agaaattttc ttagataata ttgaattaaa tcaattagaa gaacttatga
     6421 aaataaaaat tatcccttgc ttgaatgaag gtaaagactt tgtggataaa attttaaaat
     6481 agaaaacaaa agatggaagg agaaacaaaa tgagtataag taggccagtt gtagctgtag
     6541 ttgggagacc aaacgttggt aagtctacaa tatttaataa atttgcagga aaaaggatat
     6601 cgatagttga aaatacacct ggagttacaa gagatagaat ttttgctgaa gtggaatggt
     6661 tagataaata ttttacatta gtggatacag gaggaattga acctgatagc gaagatataa
     6721 tattatctca aatgagaaat caagccatgc ttgcaatgga catgtcacat gtaatacttt
     6781 ttatagttga tggtaaggct ggaataacag ctgctgataa agaaattgca caattgctta
     6841 gaaagacaaa aaaaccagta atcttagtag ttaataagat tgatagtcaa agtcaatttg
     6901 ataatatata cgatttttat gagttggggt ttggaacgcc ttttgcagtt tcaggagcca
     6961 acagtatggg atttggagat ttattagatg agatagttga aaattttcca gcaggattag
     7021 atacagagta tgaagaagat ataataagag tagctataac aggtaaacca aatgcaggaa
     7081 aaagttctat acttaataag atacttggag aagaaagagt aattgtaagt ccaattgcag
     7141 gaactactag agacgcaatt gatacttatt ttgaaaaaaa tggacagaaa tttcttctaa
     7201 tagatacagc aggtcttaga agaaaaagta aaatatatga gacaatagaa aaatacagtg
     7261 taataagagc tatgagtgct gtagatagag cagatgtagt attgatagta atagatgcct
     7321 tagaaggtgt aacagaacaa gatacaaagg ttgcaggtat agcacatgat gaaggaaaag
     7381 gatgcatatt tgtaatcaat aaatgggatt taatagaaaa agataataag actatgagca
     7441 attacacaaa agatataaaa gaaaaatttc catttatgat gtatgctcca attgtatttg
     7501 tttctgcaaa gactaatcaa agaatgaaca agatactaga tacagttgaa tatgtgtcaa
     7561 atgaacattc taagagaata tcaacttcag cattgaatga agtaattgga gaagctgtaa
     7621 tgttaaatca accaccatct gacaaaggta gaagattaaa aatttactat ggaactcaaa
     7681 ctgacattag acctcctaaa attacattgt ttataaatga taaggaatta actcacttct
     7741 catatcaaag ataccttgag aataagataa gagaaaattt tggatttgaa ggaacttcca
     7801 taaagtttga gtatagacaa aaaaataaaa aataaatatc taaaatataa ctaaaggggg
     7861 gaaattcagt ggagatattt agttatataa ttattgctgt agttgcatat cttttaggaa
     7921 atatatctac ctcatacata gttgcaaagc gaattgctgg ggttgatata aggactcaag
     7981 gttctggtaa cgcaggttca acaaatgtac ttaggacact tggaaaaaga gcaggagcaa
     8041 tgacattttt aggtgatgtt cttaaaggtg tgatggctgt tttaatatca gagtttgcag
     8101 caagattggt tggaatagat actcttttag ctggatatct ggcagttata tgtgttgtgg
     8161 caggtcataa ttggccagca gttttaggat ttagaggagg taaaggtgtt gcaacttcgc
     8221 tgggggcaat gctagctgta aatccagtaa taacactaat gtgtctagca gtatttatat
     8281 tggtggtagt tataacaaag tatgtttcat taggttctgt tgtagggata ggatgctcac
     8341 caatatttat gattatggta aaaaataaag ctggtttaat agtagctttg ttcttaactg
     8401 catcagtaat atataatcac agagcaaata taaagcgatt attaaatggg acagagagaa
     8461 aaattggtca aaagaaagaa tagtaaatat attatactca ggtggtgtta aacatggaga
     8521 aagtatgtgt attaggtact ggaagctggg gaagtgcatt agctttaggg cttgcaaaaa
     8581 aaggtaatga tgtgagcatg tggactcgaa aagaggaaca agctaaaaaa ataaacagaa
     8641 ctaaagaaaa tacagactat ttaccaggag tattatttcc aaataatata actataagta
     8701 ctgatataga aaaaactata aaagattgta aggtaataat tttagcagtt ccatcacagg
     8761 ctgtaagaag tacttgccaa aaaataaagc catttataaa agaaggacaa gttattgtta
     8821 atgtagccaa aggtcttgaa aaagggactt ggcttaggtt atctcaagtc tgtgaagaag
     8881 aattaccaca aaatccatat gtaatactat ctgggccgtc tcatgctgaa gaagtagcaa
     8941 gggatattcc tactaccgta gtagttgctt ctaaagattt aaaaatagca cagatgatac
     9001 aagacttatt tatgagccct aaacttagag tttatacgaa tccagacata gttggagtag
     9061 aacttggagg agcattaaaa aatattatag catttggagc tgggatatgt gatggattag
     9121 gatatggtga taatgcaaaa gcagctctta tgacaagagg tattagtgaa atgagcagac
     9181 ttggaattgc tatgggagct aatatgtcta cctttgcagg tctttcaggt attggagatt
     9241 taatagttac ttgcacaagt atgcatagta gaaatagaag agctggtata ctcataggta
     9301 aaggtatgag tttagaagat accttaaaag aagtaaagat ggttgtagaa ggtataactg
     9361 ctactgaagt agcacatgat gtcgctgaaa aattagatat agatatgcct ataactaatg
     9421 ctatatattc tgtaataaag aaaggttcaa atcctaaaga agttggaata gaactaatga
     9481 tgagaagtaa aaagcatgaa atggaagaag tagttttagg tgatgatata taaactattg
     9541 tataataaat aataaatttg ttttaggggt gtctcaaaat gaacttttta gtttatgaga
     9601 cactcttttt gtatgaaatt aaatgtattt ttattatttc aacaataaaa aaggcttatc
     9661 tctattttga gacagccata ttttaatttt atttagcatg ttttattatg tattcaatag
     9721 aagaaatcgt tttcatagtt aattcatcat catcagaatt ttttcctgtg ttctttttta
     9781 agaataatag attttgataa aaagaaataa ttatatcatt aattatgtct aaatctttag
     9841 ctggtatata tccagtatct acaagattgt ttaatagtat cttatttctt tcctttatac
     9901 tttctcctgt aatcattaaa gatatgtcac tatcatgaac acctaaatct tctggaaaca
     9961 tatcataata ttgttttgtt atataaatcc taagggaaca actgttggaa tagaaaaaca
    10021 tatagtatct gcaagttttt cagagtatat gactgataaa tcatttgtta tcaaagatgt
    10081 tggaagtaaa atatttgaaa tgagatatgt aaaagatgta ggagacaatg ctttactaaa
    10141 atatgtttct taaaagtatc cttatatgta tataaaattt tttaactgta tttgttcagg
    10201 gattgataga acagcacaac ttcatttaga ctccaatact agaatacttc aaagaggaga
    10261 tatagtaata catagtagac aggtttggta tgaaaactat agagcagaaa atgaaagaat
    10321 ttctatctgt aaacgaaatt aatattgctg gtctttattt ttccatttta agaatattaa
    10381 cctcgaaatc caaatataat ttaataagat tagatgtata ggaggaataa tatgaataat
    10441 aacatatacg aagatatatc caaaaggact cagggggata tatatattgg agtagttgga
    10501 cctgtaagaa caggaaaatc aacttttata agaaaattta tggaaaagtt ggttatacct
    10561 aatatagaca atgaatttaa aaaggataga acaagggatg agatacctca aagtggttca
    10621 ggtaaaacaa taatgacagt agaaccaaaa tttgtaccag cagatggagt agaaataaaa
    10681 ataaaagaca cagtatcttt aaaagtaaga atggtagatt gtgttggata catagttgaa
    10741 ggagctttag ggcatgagga aggtggaaaa caaagattag tatctactcc ttggtcacaa
    10801 gaagctatga catttgaaaa ggcagcagaa ataggaacta aaaaggttat aaaagaccat
    10861 tccacaatag ggatagttgt cttgacagat ggttcagtta ctggaattga cagaaagagc
    10921 tatgtagagc cagaagagag agttatacag gaattgaaaa accttaaaaa accatttgca
    10981 gtggttttaa atacgttaag tccaaaaagt gaggaaacga gcatgcttag aagtgaattg
    11041 gaagaaaagt atgaagttcc agttcttcca atgaatgtgg ttgaaatgga agaagaagat
    11101 atagaagagg ttatggaagc tgtattatat gattttccac ttacagaaat aagaatcaac
    11161 ctaccaaaat gggttgaagg tcttgaaaga aatcattgga taaaaagtag cataataaca
    11221 acattaaaac aaagtattat agatataggt aaaataagag atatagaagg tattatgcaa
    11281 ggattttctg aattagagtt tttggaagat acaggtgttg ataatgtaga gttaggagaa
    11341 ggtgttataa atattgattt gcagacaaag caagatttat tctataatgt ccttgaagaa
    11401 aaaagtggat ttaaaattga aggagattat caacttctta gtctgataac tagactatct
    11461 aaagttaaaa atgagtatga taaaatagaa agtgctttaa tagatgccaa gataaaagga
    11521 tatggagtag tagctccttc tttagaagaa cttagtctgg aagagccaga aataatgaaa
    11581 caaggaaaac aatatggtat caagttaaaa gccaatgcac catcacttca tattataaaa
    11641 gcagatatat cgacagaggt ttctccaata gttggaaatc aaaatcaagg tgaagagatg
    11701 ataaaatatc ttatggaagt atttgaagag caaccagcag acttatggga atcaaatatg
    11761 tttggcaaat cattacatga cttagtaaaa gaacaattac aaagtaaatt atatactatg
    11821 ccagaagaaa taagagtcaa gatgcaaaag actttacaaa aaattgtaaa tgaaggaagt
    11881 tctaatatta taactatttt gttataaaaa tatagctact tattaaagtt aaacgtctgt
    11941 ttactttagt aagtagcttt ttatttatag caattaataa aagtacttat ataaattgac
    12001 aataattgat aattatggaa aaattatagt aagtgaagta tatgtttcga caagattgtt
    12061 taaagctaaa aaatttctag gagtgtaaaa tgaaaaaata tgagatattt atttttataa
    12121 gtatagtagt tttatgtttt ataagttttc catataaagt ttttggacaa gaggaaaatt
    12181 gtcttgaaga ttttgaaagg tgggttcaag aaaataaaaa taataaagaa aaaatagttt
    12241 atactctttc ttgcgatatg gtaatagacg aagagtttag attttatatt ccttatgaca
    12301 gcaattttac aatagatact aacaaatata aaatattgat aaaaaatcat gggagattta
    12361 ttattgatga taatgaacta aatataatag gtgaaggagg aaaagaagga gtaatacatg
    12421 tagaaaatgg tggaagtata agtattggta taaataacat aattgctact gacgggacag
    12481 cattatacgt tgaggaagga ggagacttat atattaaatc atcctatgaa tctgttgaaa
    12541 aaataaaggc aaatggaaaa gatgcaatag gaatttatac tgaaaatgat attagaataa
    12601 taaataagga tattgaagtg actggagagc gtgctatagg tgtttattct aaaggtgatg
    12661 ttgagataga agaatcttca ataaaggcat atagtaacaa caatgaaaat gagttattaa
    12721 gagataataa taagttggta caatcaatta tatcagaaag caagaaagtt tatataattg
    12781 atagttataa tgaacttata ccctctatag ctgaagactc tggctacaat attgtgaaat
    12841 gttgttatag ggggattggt gtatttagtg aagaaataaa agtaagtaaa gatgacaaaa
    12901 tagaaaatat aaaatttcca caatatatgg tattagaaac atctagtggt aatcaacttg
    12961 atatagatgt agaatgggat tttactgatt attatgaaaa attagaaaaa ggagaaaaat
    13021 ttaacataac aggaaagttc aaaacagaaa cattaaataa agaaaaaatt ataataaatg
    13081 atgatgtagt tcctattttg aatatatatg ttatggataa aaaaccaata gacaatttag
    13141 aattggaatt tcaaaatacg aaaaaaggat atgtagctgc tttattttat gacatgccgt
    13201 atagtgcaag taaggttttt gttgaatatt caagcgacgg aatcaattgg atatctgaag
    13261 aacaggagga tattagagac caagctatac ttttctttga agattttaag ttgcgttgct
    13321 ttagaataag agtggtagga ggattaaaag aaggatattc aaatatagtt tttaaaccag
    13381 gttttataat gggaggtggc gacaaccaag agacgccaga tgatgaacag gaaaatgatg
    13441 atattgatgg tgaccgtgga ggaggaggta gagacgaccc tgatagagat aaggatgata
    13501 acagtcaaaa tggaaataat ggaaatgata ataatgatag taataacaat caaaataata
    13561 ataatgaaaa tgataattct gatgaaaaac aaccaaatgc tccaaaagat gatgtagaat
    13621 caagtactgg tagcaatgaa aataaaaatc ctcaaggtga tatacccaaa gaaggtaata
    13681 tacctaaaga tggtaataaa gaaaataatt ctgataaaga aaaaggagtt ttcaataaaa
    13741 gctctattat agaaaatgat ggtgattatg atttaaattt aaaatcaata tttggcatca
    13801 atgaacattc ttttccttat agagaaagta tttctaataa aaatcaagat acaaataaaa
    13861 ataagtcaaa tataagtgat tacaatggaa taataaataa taattataaa ttagaatcta
    13921 aagaatataa tctaaaaaaa gacattattt ttttagcttg catactaaca ccaattatga
    13981 ttggttctat attaattgta aattctgata ctagaaaaag tataattaag ttcataaaag
    14041 aaaaaaaata atttttagaa aggtattatt tataaactca tgagattact aagtaatacc
    14101 tttcattatt atgaaaactg tatttaatgt tgagaaaaca tatataatac gtgaattttc
    14161 taaatagtat aaaaataata gaatattgaa aaaaaattga caaatgaatg tataggtatt
    14221 aaaatatatt aaatatatac ataaaaagtg atttattaac aaataaatga aatgtatgtt
    14281 aatacatagt tcaatttgta aaaatataag attcataaaa aaataaggga ggatattatt
    14341 atgaaagcaa ttttaacagt aattggaaag gataaaccag gtatagtggc aggaattagt
    14401 gatgaattat acaagcaaaa tatcaatata gtagatatat cacaaaagat attacaggat
    14461 tattttacta tgattatgat agttgactta gaaaaatcat taaattcttt tgagaaaacg
    14521 gttgaggact tggttgaaag aggtaagaaa ttatcagtag atgtaaaaat tcagcgagaa
    14581 gaaatattta attcaatgca taatctgtag tagacagtaa ggggaggtga taacagtgaa
    14641 tgtaggaaat atacttgaaa caataaagat gatagatgaa gaacagttgg atataagaac
    14701 tataactatg gggatttctt tattagattg tatagaccct gatggagata aagctagaga
    14761 aaaaatatac aataagatta tgagctctgc taaagatttg gtgaaggttg gaaaggatat
    14821 agagcgtgaa tttggtatcc caattgtaaa taaaagggta tcagtaactc caatttccat
    14881 tattgcaggt gcaacagatg aaagtgacta tgtaaagttt gcacagacat tggataaggc
    14941 tgcagaggat ttaggtatag attttattgg tggattctca gcattagttc aaaagggata
    15001 tacaaaaggt gataagatac taattaagtc aattccaaaa gctttagcaa gtactaataa
    15061 agtatgtgct tcagtaaatg taggctctac aagatgtggt ataaatatgg atgcagtgag
    15121 agaaatggga gaaatcataa aagaaactgc agaattaaca aaagatgcaa aaggttttgg
    15181 ttgtgcaaaa ctagtagtat tttgtaatgc agtagaagat aatccattta tggcaggagc
    15241 tttccatggc gttggtgaag ctgatagaat tataaacgtt ggtgtaagtg gtcctggtgt
    15301 tgtcaagaga gctctagaga aagtaaaagg agagccattt gatgttgttt cagaaacagt
    15361 taaaaagaca gcatttaaaa ttacaagagt tggacaactt gtggcaaagg aagcttctag
    15421 tagattgaat gttccatttg gaataataga cttatcatta gctcctacac ctgctattgg
    15481 tgattcagta gcaaacatat tagaggaaat gggtcttgaa gttgttggta ctcatgggac
    15541 tacagcagca ttagcacttt taaatgatgc tgttaaaaaa ggtggagtaa tggcatgttc
    15601 acatgttgga ggacttagtg gagcttttat accagtatct gaagatgcag gtatgattga
    15661 tgctgttatc aaaggcgctt taagtataga taagcttgaa gcaatgactg ctatttgttc
    15721 tgtaggtctt gacatgatag ctgtgccagg aaatactaca gcaggtacgc ttggagccat
    15781 gatagctgat gaggcagcaa taggtatgat taataataag actacagcag ttagaattat
    15841 accagcccca ggatgtgatg taggtgatat ggtagaattt ggaggtcttt taggacgagc
    15901 accagtaatg cctataaata caaattcatc tgaactattt actcaaagag gaggaaggat
    15961 tccagcccct atacattcat ttaaaaatta gatatattac atatgataat atttaattaa
    16021 atatttatat ggttaaatta aataagtata tgtatttaaa tattatagtt aagataataa
    16081 attttaaaaa ttagttgtta agattgtggc aaaaataaag cgatacatgg acatttaaaa
    16141 ggtcatgtat cgcttttatt ttaataatat ctaattaata taaagttttt tatatatcta
    16201 aaaagttatg ttatttaata aa
//
LOCUS       DAFNNB010000086        16166 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0087, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000086 DAFNNB010000000
VERSION     DAFNNB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16166
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0087"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            594..1187
                     /locus_tag="L3M60_002275"
     CDS             594..1187
                     /locus_tag="L3M60_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6226935.1"
                     /translation="MNYTIEPTQLNKMYKDKAVINNINIHVRKEVIYGFVVQNRAGKS
                     TVMKMLLNLTKSNSGEIIIFGKKLEETDFEMLKKISTIIENTYFYENLTDKQNLDLHC
                     EYMGYYNKEHISEILKCVGLSKQSSKKVSKYFLGIKQCLLIARAILTKPELLILDESI
                     NALDPEGIREMRELFNKLNTDYITTIFTSSHILSEVE"
     gene            2315..2965
                     /locus_tag="L3M60_002276"
     CDS             2315..2965
                     /locus_tag="L3M60_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226936.1"
                     /translation="MKVKSNLDEILYHKIIESLIRGEYSVGQKILLNDLCEKFEVSRT
                     PVVQAVKMLNKDGVLTIMTNGKVYVPEYEYDMVKQVCETRTLIETYALEKMMQEKEDV
                     FQQKLDVIKRYSDKCEEYYRQEKSVELALADLELHKAIVEGANNEILNDVYVGIQGRF
                     IVVNYLIRPLKNRNYEGTVQDHFEILKFIEDRDTKKAVDKLRNHIQGTIKRFCEDE"
     gene            3256..5322
                     /locus_tag="L3M60_002277"
     CDS             3256..5322
                     /locus_tag="L3M60_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBN6226937.1"
                     /translation="MKEVVRSLKKITKERIENTMKKLFTSETFLEYQFISDCQISPDG
                     GYTAFIVKKADIKENAYTSQVYILNNKSGELKQITSINSVGAYAWEDENTILFPALRN
                     EKVKEAVKDGKQCMSYYALSLNGGEAEELFRLPIKGGKLNPIGKGLYAVIDSYDNDRP
                     IVEGLSEEEQQKKINAYNNRHYEHFKEIPYAVNGEGYVSRKRKRLYLYDSNINELKAI
                     TAPMFNVVGMKISDGKILYVGQEFKDVKGLKNGVYVFDTNTNTNVCILEKDKYIVKGF
                     ELYQNQVILNLTDALSYGNGENGDFYTIDIDTKEMKLLSEHQHHCIGNTVTSDVKMGV
                     GQTTKVDGEYIYYTSTVDMDCIIERIHIPTGKQEKVTQTGSVDFIDVKDGNIVCVAFL
                     GDRLPEVYTVENGTLCKKTHLNDHILEEYKISEPEYIESKGSSKWEIQGYVYKPVDYE
                     VGKKYPAVLVIHGGPRLTYGPYFMHEMQVFASAGYFVFFCNPRGSEGRGNAFADIRKQ
                     FGDIDYIDFMEFTDTVLEKYTDIDKTKLAVEGGSYGGFMTNWIIGHTNRFAVACAQRS
                     IANWSGMEGTTDIGYYFCKGQTGASHMENHELQWKQSPLAYADKCVTPTLFLHGEKDY
                     RCYMQEAFQMFSALKIHGCPTKLCLFEGENHELSRSGRPKQKLQRLVEMLDWFSVYIK
                     KDNIGQ"
     gene            5341..7296
                     /locus_tag="L3M60_002278"
     CDS             5341..7296
                     /locus_tag="L3M60_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HBN6226938.1"
                     /translation="MEKIKIENSDIFEYIFPHDISCSPDGKHIAYIISNINEEKDCYE
                     HDLYVMDIKTEKQIHMTQTKDVTSFSWISNTELLFTSKRNKPKAGTTDFYTISIEGGE
                     AKKAFSIPKACSVPVSLGNKLWLLTTKNPTDSKKSEPDRAVEGIDYWTFTDKPFIRDG
                     ENFSQRRRVTLELYQEGENTTKAITPKFCEVAGIDVSSDKKRILYVGQIYEDCATPFS
                     GLWEYHIESGETKELVPQGKYQISLAKYIGENKVMLQASTLDRSITQNHDIFILDLST
                     SEISMIASLDGMYATLLDVDAVYGGGRSNKVIGDKFIGARICRTMTEFDEFDTNTGNI
                     RIITKVDAFTSFDIYDNTMYTVMLKDYELAEIYSIDMTTGTMKKMTAFSKPYLDTHKV
                     SLPEKLTFIAKNKEEVDGFVIPPIDAKEGEKYPAVLFIHGGPKWAYGYMFTHLKQCVT
                     SKGMYVIYCNPHGGDGYGEKFLEMVERWGYVDYEHLMEFVDICIEKYPGIDAERLGVA
                     GGSYGGYMTNWIIGHTDRFKAAASQRGISNLITASLIIDFGDRIMKQTCGDKTPWNHE
                     EVLWNHSPIKYVKNVKTPTLFLHSDRDYRCFMGDTFQMFTALKQLGVDTEMYLFHGDT
                     HGLSRNGRPSNRIVRVNAIVDWFERYL"
     gene            7312..8502
                     /locus_tag="L3M60_002279"
     CDS             7312..8502
                     /locus_tag="L3M60_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBN6226939.1"
                     /translation="MSDMLLWFSVVLVLFAIGDLIASKTKAKVSAVFVTLLLFLILFV
                     TKAIPADIIEKAGMTSAASWSVPMIMFSMGTMLNVKQFIDEWRTVLTAWLGIVAVIVC
                     VSLCIPLFGKSTVLTSIPVINGALPATTIMTQAALEKGLTLAAATATVVFAIQKFIGT
                     PIASRAALQEANRLLVEYHEAKSKGIDLANVDTDKKEAESTGTKVKQAFCEKYDKYYS
                     TNVCIFFIALFSYLGYELSEIIHVNYSIVCLVVGVLVTRIGIVPKDILEKGKIKGFIN
                     MVVFAAVIPSLAKVSLTDLISLFVPIVGMFAASIIGIFLMMKVLPGWKIIGSKPLAFG
                     VGFCQMLGFPTTYLISNEVCNAVGETEEERAYLMSKIMPKLVVGGMACMISIVVAGLM
                     VPML"
     gene            8953..9327
                     /locus_tag="L3M60_002280"
     CDS             8953..9327
                     /locus_tag="L3M60_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226940.1"
                     /translation="MKVLISNTSDKPLYQQIKEQIKEAIFTEELKEGDAMPSIRNFAS
                     DLKVSILTIRRVYEELENEGFLTRQVGIGTFISTGNLEILRDSKRRIVEQKMADMIKD
                     AKALGITQIELQEMMDILYEED"
     gene            9329..10186
                     /locus_tag="L3M60_002281"
     CDS             9329..10186
                     /locus_tag="L3M60_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226941.1"
                     /translation="MENILEVYNLNKTYTDFSLSNISFALPEDCITGFIGVNGAGKTT
                     TIRSILDVIKRDSGKIKIFGKDFNGNEKELKNRIGVVFDDSYFYDELSMAEMKSIIAP
                     SYDNWCEEDYQKNMKHFNLNPKQKIDTLSKGMKMKFALALALSHKAEFLIMDEPTSGL
                     DPLIRSQILDILTEYMKKCGRGVFFSTHITSDLDKIADMIVMIDNGRIILEREKDTLL
                     EEHRKVKGDIKFLNDEIKKLFLNLEITGYGFIGITSKINEVQKNMPDILIERPTIEDI
                     MLAYIERRE"
     gene            10189..10827
                     /locus_tag="L3M60_002282"
     CDS             10189..10827
                     /locus_tag="L3M60_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBN6226942.1"
                     /translation="MLIHLVKKDVLIVKKYVLFILFLAIGIPLFILWRLPPFAQILGF
                     VLSIIFSEFMLCQYLLLKESQYSKASVLLCSTPYPRRGLVQSKYVLFIMVFIYCTLVY
                     WIENLLIPQMGTFDLTYILIVLLISSIIYGVYMPVQYKLGYETTKAFFVVIIMASSFV
                     LPLIIGHSNFEVPFIYQYSSMIVNIVLFLLSLIILLASMFISIKIFSNKELV"
     gene            11783..12517
                     /locus_tag="L3M60_002283"
     CDS             11783..12517
                     /locus_tag="L3M60_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6226943.1"
                     /translation="MNLTKGLDVLKISSNVFGEDKVMYIPVIYTEDEATLIDTGLPGQ
                     GDLIVDALNKSNTSFDKLKNIIITHHDIDHIGNVNYLREKSKNNIKVYAYKSEVSYIT
                     GEETPFKLYMLEQMVDKIGDKMLSMLNVMGLGFKSSYTKVDVSLDNHEFLNLGEEIEV
                     IHTGGHTRGHICLYLKESKVLIAGDLLQIDNGELKPVDVMHSNKQELKDAIKNISNYD
                     IEAIVFSHGGLYQENIIETLNNIIIE"
     gene            13549..14805
                     /locus_tag="L3M60_002284"
     CDS             13549..14805
                     /locus_tag="L3M60_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HBN6226944.1"
                     /translation="MITKFKTLIWIKILIRIKSVYKAYGGLFLLGIIGIPIGAIIGLI
                     DTVFGTVLLKITDIRETYPMYLIPFLSVVGVVIAYCYFKFGGKSSKGMNLIFEVGHGE
                     EEIIPLRLIPFIISGTWLTHLFGGSAGREGVAVQIGATFSHWIGKRLPIKNASSIFLV
                     TGMAAGFAGLFGTPIAAILFAMEVLVAGSLEYQSLFPAFIASFTASEVTKALGLEKFS
                     FALSSKVVFDLSIFWKFIALGIIFGMVGGAFAWCLKLSKRKICNRLKNPMIRIAIIGA
                     CISILFLLFDKGRYSGLGTNLIQNSFYGGEIYSFDWLLKFVLTILTLSAGFQGGEVTP
                     LFSIGASLGVLLAGFFNLPIELVAALGYASVFGSATNTFFAPVFIGAEVFGYSYLPYF
                     FVVCAISYVFNMDKSIYSLQKISTKQ"
     gene            14848..15229
                     /locus_tag="L3M60_002285"
                     /pseudo
     CDS             14848..15229
                     /locus_tag="L3M60_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895690.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
     gene            15420..15800
                     /locus_tag="L3M60_002286"
     CDS             15420..15800
                     /locus_tag="L3M60_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6226945.1"
                     /translation="MYLKKLSKLELVIMKFIWNLDIKTNSCEIIDYMKEEHNLSEKVA
                     LKTLSKLSKKRFLYVQETDKCMYYTVAIKEKAYLEFISRNVQNLLKNNFIRNLLVSFH
                     EEDLTEEKIKSLENWVVNWEEACI"
BASE COUNT         6063 a         1764 c         2769 g         5570 t
ORIGIN      
        1 tataaaactg ggatgtggtt tcttttttat gtataaaata attttttagt aaaagttaaa
       61 aagtatttta aaagatagat ggtactattt tgattactat atttgataat tacatacaag
      121 ataatatgaa aattatgatg tctaaaccct ttagagtctt gacaataaat tttagataat
      181 agataaagaa ttttttaaaa atttaattat ttggagaaat aattctaata gtattttgta
      241 tttagcttaa cgattcggag aaataattct aatggcattt tgtatttaat ttaacgtttt
      301 agagtaaagt tctaatggta ttttgtattt aacctaataa ttcagagtaa taagtctaac
      361 tgtattttgt atttaactta atgatttgga gtaataaggt taactatgtt tttgtatcta
      421 atttaattat ttacatctat gagattgact agtttttata tctatttaat ataaatagta
      481 gacctaatat aaaaaccgtt tttacagtaa aattgaaaaa ttagaaaaaa gttagatttg
      541 aaagctaaaa aagataactt tattttgtat actattttta gaaaagaagt attatgaatt
      601 ataccataga gcctactcaa ttaaataaaa tgtataaaga taaagctgtt attaataaca
      661 ttaatattca tgtaagaaag gaagtcattt acggatttgt cgtacaaaat agagcaggta
      721 aaagtacagt gatgaaaatg ttgttaaacc taactaagtc aaattctgga gaaataatta
      781 tattcggtaa aaaattagaa gaaactgatt ttgaaatgtt aaaaaagatt agtactatta
      841 ttgaaaatac ttatttctat gagaatttga ctgataaaca gaatcttgac ttacattgtg
      901 aatatatggg ctattataac aaagaacata tatcagagat attgaaatgt gtcggattgt
      961 caaaacaatc aagcaaaaaa gtttcaaaat actttttagg tataaaacaa tgtcttttaa
     1021 tagctagagc aattcttact aagccagaat tattgattct tgatgaatct ataaatgcac
     1081 tcgacccaga gggaataaga gaaatgcgag aattattcaa taagttaaat acagattata
     1141 taacaactat atttacttct agtcatattc tttcagaggt agaataaatt gcggatacta
     1201 ttggcattat tcaagatggt aaacttatta aagaaatttc tatgtcagat atacacaaat
     1261 atcaaacaga ttatgttgaa gtagatgtag ataatgtgga gcttgttgga tacttgttag
     1321 aaaaataatc ttcctataaa aattgtacct tgaggtagtg aggacttgta tagaaactcc
     1381 ttaaattctt atagtatagg taattatgtc cctttaattg aatatttatt aagtttagaa
     1441 gattttagag aagtatataa aatgttatgg aaataattgt ttgtagcaaa aaaatatgtt
     1501 ttttatacaa ttatgttttt acttatagta tttaatgcta atttcttaat cattcttagt
     1561 ttgaactata cccactattt taaccatata aaatagtggg tatagtttaa ctagttattt
     1621 ataactattt ttacatatct taattgttcc ctaatagggg gatagtccat aaaactaaaa
     1681 gtggttttta attttatcat ttataacttg ctattacctc aaaaaaatgc ttatcattat
     1741 aattttcata aaagttacct atttttttca actttttttc aactagctaa atcaattcta
     1801 ttagtgttaa tcttttatgt ttcaaactta taatacaaga aaataaaaaa taaaaaattt
     1861 taaatttttt atttattgcg aaaaaaataa aaaggtgtta tactttttgt aacatattat
     1921 tccaattatt aagagatagt aagcatttat agtttaattt gtagtacaat agcttaattg
     1981 gcagtacaat gatttaatcc atagtacaat agcatgactt gtagtacaat ggttcaaact
     2041 atagtacaat aacgtgattt gcagtatgat ggtttaagct atagtacaat agcttgtttt
     2101 gtagtacaat gatttaatcc atagtacaat agcatgactt atagtacaat ggtttaaact
     2161 gtagtacaat aacttgactt gcagtacaat aatttaatct atagtccaaa agtactatca
     2221 aataagaatt tctactttaa aaacgttact aaaagtaatt aaggtcaagt agttcaatat
     2281 aatatggcaa aagaatagaa ataaggtgag agtagtgaaa gtaaagtcaa atttagatga
     2341 aattttatat cataagatta ttgaaagtct tattcgtggt gaatactctg taggtcaaaa
     2401 aatattacta aatgaccttt gtgaaaaatt tgaagtaagc agaacaccag ttgtacaagc
     2461 agtaaaaatg ttaaataaag atggtgtgct tactattatg acaaatggta aagtgtacgt
     2521 accagagtat gaatatgata tggtaaaaca agtttgtgaa acgagaacac tgattgaaac
     2581 atacgcttta gaaaaaatga tgcaagaaaa ggaagacgtt tttcaacaaa agttagatgt
     2641 gataaaaaga tactctgata aatgtgagga gtactatcgt caggaaaaat ctgtagaact
     2701 tgcactagca gatttggaat tacataaagc cattgtagaa ggggcaaata atgagatttt
     2761 aaatgatgta tatgttggta tacaaggaag atttatagta gtgaactatt taattcgacc
     2821 attaaaaaat cgtaattatg aaggaacagt acaagaccat tttgaaatat taaaattcat
     2881 tgaagacaga gatacaaaaa aagcagtaga taaattaaga aatcatattc aaggaactat
     2941 taaaagattc tgtgaagatg aataataagt tttgaagtta ctatatatac ttatctatta
     3001 agttgctaaa tgtagtctaa gtataatcta aatgtagttt aaacataatc taaatacagt
     3061 gtaaataagt ctaagtataa attaaatata gattaagtat ggattaagta tggattaagt
     3121 accaattaag catgaattaa atatacttta aattacaaat attataagga agccataagg
     3181 cttcctaaaa aaataacctt tataaaattt tttattttaa atttaaaatt ttttatttta
     3241 aatttaaaat aaaaaatgaa agaggtggta agaagtttga aaaaaattac taaagaaagg
     3301 atagaaaaca ctatgaaaaa gctatttaca agtgaaacat ttttggaata tcaatttata
     3361 agtgattgcc agatatcacc agatggagga tatacagcat ttattgtaaa aaaagctgat
     3421 atcaaagaga atgcttacac aagccaagtt tatatactaa acaataaaag tggagaatta
     3481 aaacaaataa cgtctataaa ctctgttgga gcatatgcct gggaagatga aaatactatc
     3541 ttgttccctg ctttacgaaa tgagaaagta aaagaagctg tcaaagatgg taaacaatgt
     3601 atgagctact atgctttatc tttaaatggt ggagaagcag aagaactttt tagactccct
     3661 atcaaaggtg gtaaattgaa ccctatagga aaaggtttgt atgcagttat tgattcttat
     3721 gataacgacc gacctattgt agagggattg tcagaagaag aacaacagaa aaaaataaat
     3781 gcttataaca acagacacta tgaacatttc aaagaaattc cttatgctgt taatggggaa
     3841 gggtatgtta gtagaaaaag aaaacgctta tatctatatg acagtaatat aaatgagttg
     3901 aaggcaatta cagcacctat gtttaatgtt gtaggtatga aaataagtga tggaaaaatc
     3961 ttatatgtag ggcaagagtt taaagatgta aaaggtttga aaaatggagt ctatgtattt
     4021 gatacaaaca caaacacaaa tgtttgtata ttagaaaaag ataagtatat tgtaaaaggt
     4081 tttgaattat atcaaaatca agtgatacta aacctaactg atgctttatc ttatggaaat
     4141 ggagaaaacg gagattttta tactattgat attgatacaa aagaaatgaa acttctttca
     4201 gagcatcaac atcattgtat tggaaataca gtaacatctg atgttaaaat gggtgttggt
     4261 cagacaacaa aggtagacgg agaatatatt tattacactt ctacagtaga tatggattgt
     4321 attattgaaa gaatacatat tccaacagga aaacaagaaa aagtaacaca gacaggttca
     4381 gttgatttta tagatgtaaa agatggcaat attgtatgtg tagctttttt aggagataga
     4441 ttaccagaag tttatacggt agagaatgga acactgtgca agaaaacaca tttaaatgac
     4501 catattttag aggaatataa aatttctgaa ccagaataca tagagtcaaa aggaagttct
     4561 aaatgggaaa tacaaggcta tgtatataaa ccagtagatt atgaagttgg gaaaaaatat
     4621 ccagctgtat tagtgattca tggaggaccg agattgacat atggaccata ttttatgcat
     4681 gagatgcagg tatttgcaag tgcaggttac tttgtattct tctgtaaccc aagggggtct
     4741 gaaggtcgtg gcaatgcctt tgcagatatt aggaaacagt ttggagatat tgattatata
     4801 gattttatgg aatttacaga tactgtactt gaaaaatata cagacattga taaaacaaaa
     4861 ttggccgtag aaggtggaag ttatggtgga tttatgacga attggataat tggtcataca
     4921 aaccgttttg ctgttgcttg tgctcagcgc tctattgcaa actggagtgg catggagggt
     4981 acaacagaca ttggttatta tttctgcaaa ggacaaacag gtgcttcaca tatggaaaac
     5041 cacgagcttc aatggaaaca gtcacctctt gcttatgctg ataaatgtgt tacaccgact
     5101 ttatttttac atggtgaaaa agactataga tgctatatgc aagaagcatt ccagatgttt
     5161 tcagcattga aaattcatgg atgtcctaca aaactttgtc tatttgaagg ggaaaaccat
     5221 gaactgtctc gttctggtag accaaaacaa aagctacaaa gattagttga aatgctagat
     5281 tggttttctg tgtacataaa aaaagataac ataggacagt agagaaagga gaatataaaa
     5341 atggaaaaaa taaagattga aaattctgat atttttgaat atatattccc tcatgatatt
     5401 tcttgttcac ctgatggaaa gcatattgct tatattatat caaatatcaa tgaggaaaaa
     5461 gactgctatg aacatgactt atatgtgatg gacatcaaaa cagaaaaaca aatacatatg
     5521 actcaaacaa aagatgtaac atcatttagt tggatttcta atacagaact actttttaca
     5581 tctaaaagaa ataagccaaa agcaggtaca acagattttt acactatttc tatagaagga
     5641 ggagaagcaa aaaaagcatt ttctatccca aaagcttgta gcgtacctgt atcacttgga
     5701 aataaattgt ggttattgac aacaaaaaat ccaacagatt ctaaaaaatc agagccagat
     5761 agggcagtag agggtataga ctattggaca tttacagata aaccttttat aagagatgga
     5821 gaaaactttt ctcaaagaag acgtgtaaca ttagaactat atcaagaagg tgaaaataca
     5881 acaaaagcca ttacaccaaa attttgtgaa gtggcgggta tagatgtttc ttctgataaa
     5941 aaaagaattt tatatgttgg tcagatttat gaagattgtg caactccttt ttctgggctt
     6001 tgggagtacc atatagaaag tggtgaaaca aaggaacttg taccacaagg aaaataccaa
     6061 ataagtttgg caaaatatat tggcgaaaat aaagtaatgt tgcaagcttc aacattagac
     6121 cgttctatta cacaaaatca tgatattttt atacttgatt tgagcactag tgaaataagt
     6181 atgattgcta gtctagatgg tatgtatgct acattactgg atgtagatgc agtatatggt
     6241 ggtggacgta gtaataaagt tataggagat aaattcattg gtgcaaggat ttgcagaact
     6301 atgacagagt ttgatgagtt tgatacaaat acaggtaata tacgtattat cacaaaagta
     6361 gatgctttta catcttttga tatttatgat aacactatgt atacagtaat gttgaaagat
     6421 tatgagttgg cagaaatata tagcattgat atgactacag gtacaatgaa aaaaatgact
     6481 gctttttcta aaccctattt agacactcat aaggtttcat tgcctgaaaa attgacgttt
     6541 atagcaaaaa ataaagaaga agttgatggt tttgttatac cacctataga tgctaaagaa
     6601 ggcgaaaaat atcctgctgt attgttcatt catggtggac ctaaatgggc atatggctat
     6661 atgtttacac acctaaagca gtgtgtaaca tcaaagggaa tgtatgtgat ttattgtaac
     6721 cctcatggcg gtgatggcta tggtgaaaaa ttcctggaaa tggtagaaag atggggatat
     6781 gttgattatg aacatcttat ggagtttgta gatatttgta ttgaaaaata tcctggaatt
     6841 gatgcagaac gcttaggtgt tgcaggagga agttatggcg gttatatgac aaactggata
     6901 attggtcata cagaccgttt caaggcagct gcttctcaaa gaggtattag taacttgatt
     6961 acagcttctt taattattga ctttggggat agaattatga agcagacttg tggagataag
     7021 acaccttgga atcatgagga ggttctttgg aatcattcac ctattaagta tgtgaaaaat
     7081 gtgaaaacac caacattgtt cttgcatagt gatagggatt atagatgttt tatgggggat
     7141 actttccaaa tgtttacagc attaaaacag cttggtgtag ataccgaaat gtatttgttc
     7201 cacggagata cacatggatt gtctagaaat ggaagaccat ctaatagaat cgtacgtgta
     7261 aatgctattg tagattggtt tgaaagatat ttataataag ggggttattc tatgagtgat
     7321 atgttattat ggtttagtgt agtattagtt ttatttgcta ttggagattt gattgcaagt
     7381 aaaacaaaag caaaagtatc agctgtattt gttacattac ttttattttt aattctcttt
     7441 gtcacaaaag caataccagc tgatattatt gagaaagcag gtatgacctc agcagctagt
     7501 tggagtgttc caatgattat gtttagtatg ggaacaatgt tgaatgttaa acaatttata
     7561 gacgaatgga gaaccgtatt gacagcatgg cttggaattg tagcagttat tgtttgtgtc
     7621 tcattatgta ttcctttgtt tggaaaatca acagtattaa caagtatacc agttataaat
     7681 ggagctttac ctgctactac tattatgaca caagcagcat tagaaaaagg attaacactt
     7741 gcagcagcta cagcaacagt tgtatttgca attcagaagt ttattggtac accaatagct
     7801 tcaagagcag cactacaaga ggcaaaccgt cttttagtag aatatcatga agcaaaatca
     7861 aaaggcattg atttagcaaa tgttgatact gataagaaag aagcagagag tacagggaca
     7921 aaagtaaaac aagctttctg tgaaaaatat gataaatatt actctacaaa tgtctgtata
     7981 ttttttatag cactattctc ttatctagga tatgagcttt cagagattat tcatgtaaat
     8041 tattctattg tatgtcttgt tgttggtgtt cttgtaacac gtattggtat tgtgccaaaa
     8101 gatattttgg aaaaaggaaa aatcaaggga tttatcaaca tggttgtgtt tgcagctgtt
     8161 attccttctt tagcaaaagt ctctttgaca gacttaatta gtctttttgt accaattgta
     8221 ggtatgtttg cagcctctat cattggtata ttccttatga tgaaggtatt accaggttgg
     8281 aaaattattg gttctaaacc tcttgctttt ggagtaggct tctgtcagat gttgggattc
     8341 ccaactacat atttaatttc aaatgaagta tgtaatgctg ttggagaaac tgaagaagaa
     8401 agagcatatt tgatgtctaa aattatgcct aagctggtag ttggtggtat ggcttgtatg
     8461 atttctattg tagtagcagg tcttatggta ccaatgttgt agatagattt tatagttaaa
     8521 aaagtaaatt taatttatag tagaatataa aaaaagaagt gccttaaagt gaactcttag
     8581 tccataaggc acttttttgt atgaatttga atatatttta atcattttaa tattgataaa
     8641 gagacttata tctagtttga gataagtctt tttaattatt aaagttattt tttatcttat
     8701 ttgcataatt ttatactttt caagtataca attttatttt taatataact tttttaatta
     8761 atttccaaat tatataagca taacatatgc ttttaacagc tttactaata gataaggtat
     8821 agttaaattt agtaatgaaa agtttaactt atattgagtg taaatctttg tattgacaaa
     8881 aaaagtgtac taagtgtata gtgtgtatat aataacatca cattgaaagt gataaaaagg
     8941 aggtgataaa aaatgaaagt cctaatttct aacacatcag ataagccatt atatcaacaa
     9001 attaaagagc aaattaaaga agccatattt actgaagaac taaaagaagg ggatgcaatg
     9061 ccctccataa gaaattttgc tagtgattta aaagttagca ttttaactat tagacgagtg
     9121 tatgaagaac ttgagaatga aggctttctt acacgtcaag ttggaatagg gacctttatt
     9181 tcaactggaa atttagaaat attaagagat tcgaagagac gtatagttga acaaaaaatg
     9241 gcagatatga taaaagatgc aaaagcacta gggattacac agatagagtt acaagaaatg
     9301 atggatattc tatatgagga ggattaatat ggaaaatatt ttagaagttt ataacttaaa
     9361 taagacttat accgattttt cattatctaa tataagcttt gcgttaccag aagattgcat
     9421 aactggattt ataggtgtta atggagcagg gaaaacaaca acaatccgtt cgatattgga
     9481 tgttataaag cgcgatagtg gaaaaataaa aatctttggt aaagacttca atggaaacga
     9541 gaaggaatta aaaaatagaa ttggggttgt ctttgatgat tcatattttt atgatgagtt
     9601 gtcaatggca gaaatgaaga gtattattgc accttcatat gataattggt gcgaagaaga
     9661 ttatcaaaaa aatatgaagc attttaatct taatccaaaa caaaagattg atacactttc
     9721 aaaaggaatg aaaatgaaat ttgctttagc attagcatta tcacataaag ctgaattttt
     9781 aattatggat gaacctacaa gtggcttaga cccattaatc cgaagtcaga tacttgacat
     9841 actaacagaa tacatgaaga aatgtggtcg tggtgttttc ttttccactc atataacatc
     9901 tgatttagac aaaatagcag atatgattgt tatgattgat aatgggcgta ttatattaga
     9961 acgtgaaaaa gatactctct tggaagaaca tagaaaagta aaaggggata ttaagttcct
    10021 aaatgatgaa atcaaaaaat tatttttaaa tttggaaatt acaggttatg gttttatagg
    10081 gattacctca aaaattaatg aagttcaaaa aaatatgcca gatattttga tagagcgtcc
    10141 tacaattgaa gatattatgc ttgcgtatat tgaaaggaga gagtaattat gttgattcat
    10201 ttagttaaaa aagatgttct gattgtaaaa aaatatgtat tgtttatatt atttcttgct
    10261 ataggaatac cattatttat attatggcgt ttacctccgt ttgcacaaat attgggattt
    10321 gtactgtcaa ttatattttc agaatttatg ctgtgccagt atctcttatt aaaggagagt
    10381 caatattcaa aagcatctgt attactttgt tcaactcctt atcctagacg tggcttagtt
    10441 caatctaagt atgttttatt tataatggta tttatctatt gtacattagt ttactggatt
    10501 gaaaatttac ttattccaca aatgggaact tttgacttaa catatatttt gattgtttta
    10561 ctaataagtt ctattattta tggagtttat atgcctgttc aatataaatt aggatatgaa
    10621 acaacaaaag ccttttttgt ggtgataatc atggcttcat catttgtatt accattaatt
    10681 ataggtcaca gcaattttga agttccattt atatatcagt attcatccat gatagtgaat
    10741 attgtattgt tccttttaag cttaattata ctgttagctt caatgtttat ttctattaaa
    10801 atttttagta ataaggaact tgtataaggt aaggggaaat tttatatgcc acatttgcca
    10861 gattgggctg ttttactttg tatcttactt ccaatagtat ataaagcttg tcagatattt
    10921 aaaagtaaaa aatagagtac atatgtatat tgacattttt attgagtgaa gttagatgtt
    10981 atgaatttag aaggttactt gaaaataaag ttacttggtg tgtgagagta acatcataat
    11041 attatagatt atatgtttct tgtgaatcaa tttgtattct ttgatagagg atgtcaagta
    11101 attttatttt tcattgatta tttgtcaatt atgggtataa ttgtgtatta tctttcaagg
    11161 tgtttttgaa aatttatatc gtcaaaatat aaatctaagg actcctttaa ataaagtttg
    11221 ttaatcttag aaaactaata aaaagtagaa aactaataaa aataggaaat gaataataag
    11281 aggctgtcta aaaatagaga taagcttctt tttcattgtt aaaataatga aaatacagtt
    11341 gatttcatat aaaaagagta ctttatgaac taaaagttta ttttgaaccc aatttttatt
    11401 aaacaatatt ttattcttct caaatagtca tctatataca gtattgtatt ttttacttat
    11461 ttataattta ttttcttatt atagtttttc tatatttata ttttctatat tactttacgt
    11521 ttaacaacgt acaaattatt aaaaataaaa tagttattag aaaaaaataa aagtaattgc
    11581 aaagattgca attacttact tctatattaa ttactatacc ataaaataac aaaaaaatca
    11641 agtcgagtaa tccctcaact taagtgatat actaggtatt aataattttt aattaaaaaa
    11701 atataactag aggcaaatct atattttctc aatttcttta attaaattga cctctaatta
    11761 agtaattata aggagaaaaa ttatgaactt aacaaaggga ttagatgttt taaaaatttc
    11821 atcaaatgta tttggtgaag ataaagttat gtatattcct gtaatctaca cagaagatga
    11881 agcaacgctt attgacacag gtcttccagg tcaaggtgat ttaatagttg atgctcttaa
    11941 taaaagtaac acaagctttg acaagttaaa aaatataata atcactcatc atgatataga
    12001 ccatatagga aacgtaaatt atctaagaga aaaatctaag aataatatca aagtatacgc
    12061 atacaaaagt gaagttagct atataacagg agaagaaact ccttttaaac tttatatgtt
    12121 agaacaaatg gtagataaga ttggtgataa gatgcttagt atgctaaatg ttatgggact
    12181 tggatttaaa tcatcatata ccaaagtaga tgtatcatta gataatcatg aatttttgaa
    12241 tctaggtgag gaaatagaag ttatacatac aggaggtcat actagaggtc atatatgtct
    12301 ttatttaaaa gagtctaaag tacttattgc tggagatttg ctacagatag ataatggaga
    12361 acttaagcct gttgatgtaa tgcacagcaa caaacaagag ttaaaagatg ctattaaaaa
    12421 tataagtaat tatgatatag aagcgatagt ttttagtcat ggtggattat atcaagaaaa
    12481 tataattgaa acattgaaca atataattat tgagtagtat ttattatatg attttaataa
    12541 tctacaataa tttcttaaat tacataacgt tgtatttagg tgtgaaaaat taaaatcaaa
    12601 tataaataat aaaaattaga ttgtatcaat aaaaatatcc ttataattta tttatttgga
    12661 ttctaaggat attaaatcta ttacaagtat atatatagat gtagttaatt acactacaag
    12721 ttaaagaatc taaaccaaca attaaaagaa atcaaaatat aaatccaatc aattaaattc
    12781 ttgacatata taaattgttt ttatcctttt ttgggtgtcg ataataattt ttaaaattta
    12841 atgtcaagta aacacaataa aacacagagc ttattctaaa attatggttt ttattttatg
    12901 tatacagtta tagtattgca agtagtttac aaaatggtct atatgtgtca aattgaaagt
    12961 cttgtaatac ttaagttgaa aaatatttaa catcatagta taatagggta gaaaatactt
    13021 taaatcaact ttagaataca atattataaa taatcactca gatagatatt ataaaaataa
    13081 aataaattct ggaaaaggca taacatggat gtactggata atttagatga taaattgtaa
    13141 attgtattat ttacaacata aagaatataa cataagggat aagattgaca tataaaaaat
    13201 gatataataa ttatgggaat gaggttctcc cttggatgat aatatcctaa actgccaatg
    13261 agctgatgac ttctatgata tcatagatgc caccagcttt ttttgttgtt taaattgata
    13321 aaatggaggt gaaatagtta ctaaattctt attgtttgaa tttgaataaa tatttaaaag
    13381 atagaaaaaa tggcatttta tatgctaatt actttgttag tatagagatt aagagtgtgg
    13441 ctatatgcta ttaattaaaa aaaacaatta ttatagaagc cacattgcct atagttgatg
    13501 caataatatt ttcaaaatat cttataaagc aggtagaaag gaagtaaaat gataacaaaa
    13561 tttaaaactt taatatggat taaaatatta ataagaatta aaagtgttta taaagcatat
    13621 ggaggactat ttttgttagg aattataggt ataccaattg gtgcaattat aggtcttata
    13681 gacactgtat ttggaacagt tcttctaaaa attacagata ttagagaaac atatccaatg
    13741 tatttgatac catttttatc agtagtaggt gttgttattg cttactgtta ttttaagttt
    13801 ggaggaaaga gtagtaaagg aatgaatcta atatttgagg tagggcatgg agaagaagaa
    13861 attattcctt taagattaat tccatttatc atatcaggta catggttaac ccatttattt
    13921 ggtggtagtg caggacgaga aggagttgct gttcagattg gtgctacttt ttcacattgg
    13981 ataggcaaga ggttgcctat aaaaaatgct tctagtattt ttctagtaac tggtatggca
    14041 gctggttttg caggattatt tgggacacca atagcagcaa tcctttttgc tatggaggtt
    14101 ttggtagctg gttcactgga gtatcaatcg ctttttcctg catttattgc ttcttttaca
    14161 gcaagtgaag taacaaaagc tttaggatta gagaaatttt cttttgcact ttcttctaaa
    14221 gttgtatttg acttatcaat attttggaag tttattgctt taggaattat ttttggaatg
    14281 gttggaggag catttgcatg gtgtcttaaa ttgtcaaaaa gaaaaatatg taatagatta
    14341 aaaaatccta tgataaggat tgctattatt ggagcatgta ttagcatatt attcctttta
    14401 tttgataaag ggagatattc tggtttaggg acgaatctaa ttcaaaatag tttttatggt
    14461 ggagaaattt attcatttga ctggctgtta aagtttgtac ttacaatact gactttgtcg
    14521 gcaggttttc aagggggaga ggttacacct ttgttttcta ttggagctag tcttggtgta
    14581 ttactagcag gattttttaa tttaccaatt gaattagttg cagcacttgg ctatgctagt
    14641 gtctttggta gtgctacaaa tacttttttt gcacctgtat ttataggagc agaagttttt
    14701 ggatacagct atttgcctta tttctttgta gtatgcgcta tttcatacgt atttaatatg
    14761 gataaatcta tttattcgct acaaaaaatt tcaacaaaac aataaaaaaa caacataata
    14821 tgttaatgga atctgtttag cagtattttt aagaaagtcc atatgagaga ttctgctaaa
    14881 agtgttttac ttttgggtat attattttta atgataagtt tggagaatga tttagatgga
    14941 attgtgtcat tttccggatt attagcagtt atgagtattc ttatgtccta ttaagacaaa
    15001 attgatatta aaagaaatat tattctgtat gatgggatat attccaaaag ctaaagtaca
    15061 gatagcaata ggaagtgtac cacttgcaat ggagatggta tctgggcaat ttattttgac
    15121 attagcagtt ctatctatac ttataactgt gcctcttgga acttttggaa ttgatacaac
    15181 ttataaaaaa ttattaacat ctgtaaaagg tgacagtaag caaaaataaa gattatttta
    15241 agtggtcaat tttaaaaaaa gaaccatatt atgaatacaa tgtcagaaaa tcatttgaga
    15301 gattaaagaa gatagaagtt cagtatggat tttaaggtat gtgatgatta ttagaattga
    15361 ttttgtgaaa ttattattgt aatatttaac tatatagata aagaagcgga gtgataacta
    15421 tgtatttaaa aaaattatcg aaattagagt tagttattat gaaatttatt tggaatttag
    15481 atataaaaac caattcatgt gagattattg attatatgaa agaagagcat aatttatcag
    15541 aaaaagtagc tttaaaaact ttatcaaaat tatcaaagaa aagattctta tatgttcaag
    15601 aaacagacaa atgtatgtat tatacagttg caataaaaga aaaggcatat cttgagttta
    15661 tatcgagaaa tgtgcaaaat cttttgaaga ataattttat aaggaattta ttagtgtcat
    15721 ttcatgagga ggatttaaca gaggaaaaga taaaatcgtt agaaaactgg gtagtaaatt
    15781 gggaggaagc ttgtatataa tgttattatt tttattacaa tttagataat tttggttttg
    15841 taagctctgc aactattttt agatggttgc agagcttatt tttattttta atttccttaa
    15901 gtaatactta aaagtaaacg tatttatatt tactaacttc aatcacttct aatacttacg
    15961 ttacttttta aaagtatttt gatatgtcat ctctatagta atctaaaact tgtttctatt
    16021 atttatatga agtttttaat tattagagat gattatattt aaaataaatt atcatataaa
    16081 ttatgctaac ttattagact tgtatttaaa acatatttgg cttaaattta aaaaattcaa
    16141 gttttgcata gactaaaaaa agtttt
//
LOCUS       DAFNNB010000087        16115 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0088, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000087 DAFNNB010000000
VERSION     DAFNNB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16115
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0088"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            367..1560
                     /gene="nifV"
                     /locus_tag="L3M60_002287"
     CDS             367..1560
                     /gene="nifV"
                     /locus_tag="L3M60_002287"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HBN6226946.1"
                     /translation="MCVISKDRAKEIKIVDTTLRDGEQTAGVVFANREKIMIAEMLSD
                     LGVDQIEVGIPTMGGDEKNVIKHICSRNLKSDIMAWNRAVIKDVEESISCGVDAVAIS
                     ISVSDIHIENKLRTSRGWVLENMAKTVEFAKKNGLYVSVNGEDASRADIDFLTEFINV
                     GKQAGADRFRYCDTVGVMNPFSIKNAIETLYERTNFDIEMHTHNDFGMATANALAGIA
                     AGANYVGVTVNGLGERAGNAALEEVLMALKCVYKCDLNNIDTRKFRGICEYVAQASGR
                     ILPTWKPVVGDNMFIHESGIHADGALKDPHNYEPFDPSEVNLERKIVIGKHSGRAAVV
                     NKLSEYEMYISPENATKLLNAIRATSIRLKRSLMDKEILQLYCDILAHEKGTTEEEAV
                     RGSYI"
     gene            1585..3510
                     /locus_tag="L3M60_002288"
     CDS             1585..3510
                     /locus_tag="L3M60_002288"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBN6226947.1"
                     /translation="MGDNIVYKIIKKHIVDGEAVAGSSIGIKIDQTLTQDSTGTMTYL
                     QLEAMGIDKVKTKRSVAFVDHNMLQQGFENADDHKYIQTVADKYGVYFSKPGNGICHQ
                     VFLERFSTPGDTLLGSDSHTPTAGGVGMMAIGAGGLDVALAMAGGAYYIKAPKVCKVN
                     LVGKLNNMVSSKDIILEVLRKQTVKGGVGKVYEYGGEGVKSLSVPQRATITNMGAELG
                     ATTSIFPSDEKTLEFFKSQGREDAWIELKPDADAVYDEEITINLDELKPLAAKPHSPD
                     NVDEVENIGKIKIDQVAIGSCTNSSYEDLMKVAQILKGNKVHKDVSLVIAPGSRQVME
                     MIARNGALADIISAGARILENSCGPCIGMGQSPGTDSVSLRTFNRNFYGRSGTLSAQV
                     YLVSPEVAAVSAIKGVLTDPREFDIKFTNLDVNEFLIDDSMIIKPADVGSDVEVVRGP
                     NIKPFPLNTELSQSIDGKVILKTEDNITTDHIMPSNAKLLPFRSNIPYLANYCFNTVD
                     TEFPQRAKENNGGFIVGGDNYGQGSSREHAALAPLYLGVKGVIVKSFARIHKANLINS
                     GIIPMEFCDEKDYENISLLDNLEIPNILDNLSNGILEVKNTTKGTSFKVKVELSAKEV
                     DVLKAGGKLNYTKNQAN"
     gene            3532..4527
                     /locus_tag="L3M60_002289"
     CDS             3532..4527
                     /locus_tag="L3M60_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HBN6226948.1"
                     /translation="MYKVTLIPGDGIGPEVAKAMKKVVEATGVEIEWEEVNAGEAVIE
                     EYGTPLPEYIIDSIKKNKIAIKGPITTPVGKGFRSVNVTLRQALDLYVNLRPIKSFKG
                     IKSRYEDVDLVVVRENTEDLYAGIEHKIGDYAAESIKIITRSASERIVDFACNYVKDN
                     KRKKVTAIHKANIMKMSDGLFLDVFREVASKHDVEYDDLIVDAAAMNLVLNPENYDVM
                     VMPNLYGDILSDLGAGLVGGLGIIPSANIGKDCAIFEAVHGSAPQIAGQNKANPTALI
                     QSSVMMLRYLGEYENAQKIETALEKVFLEGSKLTVDLGGSASTTEFADEVCKYIL"
     gene            4856..5329
                     /locus_tag="L3M60_002290"
     CDS             4856..5329
                     /locus_tag="L3M60_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6226949.1"
                     /translation="MLGSVEVCLIKTLDSNILREVGTLSRAVNSINDIKYKELKLQKG
                     QFTFLTRICENPGINLVELSNILKVDKATTTKAIQKLIKAGYVDKKQDEFDKRGYNLT
                     PTNKSLEVYELIIEEENRSIEICFKNFTDEEKQVVTKLLEKMSKNAENEWFKVKR"
     gene            5363..5857
                     /locus_tag="L3M60_002291"
     CDS             5363..5857
                     /locus_tag="L3M60_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6226950.1"
                     /translation="MIRKANMNDLESIMKIIKSTVKEMKTYNNTQWDENYPLEKDFVS
                     DIKKQDLYIYEVDGEVAGFICLNYEEPEEYLGLNWSSNKKAMVIHRMAVNPNFRKKGI
                     ASKLVDFAEKLARENNVLYLKSDTYSINSKMNLLLTKCGFIKIGEMSFLGKEKSFYCY
                     DKIL"
     gene            5936..6475
                     /locus_tag="L3M60_002292"
     CDS             5936..6475
                     /locus_tag="L3M60_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6226951.1"
                     /translation="MKGMIYVIEFLKKRRSIRKYKDIEVEKEKLDKILKAALLAPSSK
                     GLRTWEFIVVDDKEKLIKLSQCRTKGGGFFLKNAPLAIAIIADKEKNDVWIEDASIAA
                     SYIQLQAHELGLGSCWIQVRNRMHDDNIEADKYIREELKVPDKYSVECIISIGYPDEE
                     KKAYSDSDLDYEKVHFNNF"
     gene            6664..6975
                     /locus_tag="L3M60_002293"
     CDS             6664..6975
                     /locus_tag="L3M60_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6226952.1"
                     /translation="MGIFKTKIDEDWKVNYIKEFNEMRDSYESKLQKKQFEVDSLKSE
                     LDRLRSYKNSLKPKEKQITDDDINNIKNLRRDGLSYKEISNQTSWSKATVSRVLNGLY
                     D"
     gene            7003..7689
                     /locus_tag="L3M60_002294"
     CDS             7003..7689
                     /locus_tag="L3M60_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6226953.1"
                     /translation="MKYSYKKCIIDSILLMLIVQLLRMILNYVLLSQFEFTLENFNII
                     NLISFTLVGLSLILFLKDNSLYNKVRNRKITEAFEENKNSILIEKCKLILFVVVLSLA
                     IISTYCTKGYVFFNVTMMTLSVFIVPIFEELFFREYIWNYLSNFIKSKIKIICITSIL
                     SGIYNIGYIDIIRNYIALYNNSSYTFEVIISKIIIGTVFGIVLGLVRYRFRDVRLCIL
                     LRSLFAIFIR"
     gene            7921..8805
                     /locus_tag="L3M60_002295"
     CDS             7921..8805
                     /locus_tag="L3M60_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBN6226954.1"
                     /translation="MKFVTFCSSNEEKIGVFNSETNSIYEINSLGLSKLYTDMNDFIE
                     NVSTGDLEKIKNNSFENAKCYKLEEVKLCSPIVRPKKDIICLGLNYKDHVNEIPDGVI
                     KNVVMPDYPIYFSKRADKIIGVDDKISLHGDLVEKLDYESELAVIIGKEGINISKEDA
                     YDYIFGYTIVNDISERALQDKHVQWFRGKSLDTHTSMGPCIVHKEEFEQPLKLDISSV
                     VNGEVRQNSNTEYFIFDIPTIINDLSRGMTLKPGDIISTGTPAGVAMGMNPQVYLKHG
                     DVVECKVEGIGVLKNIVD"
     gene            8904..10079
                     /locus_tag="L3M60_002296"
     CDS             8904..10079
                     /locus_tag="L3M60_002296"
                     /EC_number="2.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolecarboxamide
                     formyltransferase"
                     /protein_id="HBN6226955.1"
                     /translation="MARELELKYGCNPNQKPSKIYMKNGELPIEVLNGKPGYINFLDA
                     FNSWQLVKELKEATGLPAATSFKHVSPAGAAVGIPLSDTLKQIYFVDDLELSPLACAY
                     AMARGADRMSSYGDFIALSDVCDKETATIIAREVSDGIIAPGYTDEALEILKGKRKGN
                     YNIVKIDENYTPEPIETKDVYGITFEQGRNEILINEDLLKDIPTDNKIFTDNAKRDLI
                     IALITLKYTQSNSVCYAKDGQVIGVGAGQQSRIHCTRLAGNKADTWYLRQHPKVLNLK
                     FKKDIGRPDRDNTIDVYLSDDYMDVLADGIWQNFFEEKPEPLTGEGKRAWLKTLTGVA
                     LGSDAFFPFGDNIERAKRSGVSFIAQPGGSIRDDNVILTCNKYNIVMAFTKNRLFHH"
     gene            10207..11061
                     /locus_tag="L3M60_002297"
     CDS             10207..11061
                     /locus_tag="L3M60_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226956.1"
                     /translation="MALNIRNYSLLCNQGTAKFNEDVVGINPFGAWILDGATGLNGKN
                     LVSTESDARWYVQWWNRYLYKNINKEESLKSIINAGIIEVKKEYELRLNGIKVEKLDL
                     PSSSIAVVKFHENKVEYFLLGDCTLFIKNGESKVIKDRSICKFDNIVFDEMSKLSNLE
                     EMAFDEIRTSVMDTIVSNRLKKNTKDGYWILDFDEEAVENSIYGYIEIKKDFQLMLTS
                     DGFTSACDRYNLIKEEELIRIAEKFGIGYIHNKVRDFEDNDYKAVKIPRFKVKDDSSC
                     LYLDIYND"
     gene            11104..12339
                     /locus_tag="L3M60_002298"
     CDS             11104..12339
                     /locus_tag="L3M60_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBN6226957.1"
                     /translation="MKILHIITQKPNSTGSGIYLSGMIKGFEKIGHKQAVIAGIDVND
                     DVNCFPSDVSFYPVKYNCGELNFPVVGMSDSMPYESTRYKDLNIDMINKLKYQFKINI
                     DRAMNDFKPELIICHHLYLLTAFVRETVKDIKVMSICHGTCLRQLNTIDLEKEYIISN
                     IRKLDLIFALHENQKYDIIKTFGVSESKVVVIGSGYNDDIFYNKNYKIKDDKIKIVFA
                     GKICKSKGLIPFIKAISKLKYSRDLIEVNFAGTGSDIESYNEIVKLASKSPFKMNFLG
                     KLEQRDLAELFNRSQIFVLPSFYEGLPVVVLEALSCGTDVITTDILGVKEWIGSEINN
                     SGKIEYVSLPFMEKEGIPKDEELHDFENNLHNAIDSKIQSLLHNSNKKTSVDMSKKTW
                     DGLAYRINEVILMDELCLV"
     gene            12441..13382
                     /locus_tag="L3M60_002299"
     CDS             12441..13382
                     /locus_tag="L3M60_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp25"
                     /protein_id="HBN6226958.1"
                     /translation="MRISKKVLALGISALLLSVSFPTSINALDKIENIKGVDKYETAG
                     LISDKQDFTTAVLINADSTMVDGIAASGLAGVNNAAILLTNKDDIPEATLQRLNRVTK
                     IYVIGGENSISKDVEEMLLMRRMQVIRIDGVDRVDTSYKIAGEIEKIKNSDKMFLING
                     FKDEADAVSVASVAYRDGAPIILTKDIPSAEEDTDLDPWFGVSPVPVYAIGGESTLSD
                     YIVSRYRATRIGGVDRYQTNKNIIEKFYNGAKEFYITSGDDLVYALVASPLAKNAPVA
                     LVSNKSDKSILSGASKVTAIGISDNSIIEQCLDAVKK"
     gene            complement(13599..13952)
                     /locus_tag="L3M60_002300"
     CDS             complement(13599..13952)
                     /locus_tag="L3M60_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HBN6226959.1"
                     /translation="MDRKRELKQLYKERKFDTGVFIIKNDITKKIYLGKSNDIKSKFN
                     SIKFQLGAGSCMIKELNNEWKKYGENAFTFEVLELIKHDDNKTKKDYLEELDILEMVW
                     LEKLKEDNTYEIYSI"
     gene            14248..15159
                     /locus_tag="L3M60_002301"
     CDS             14248..15159
                     /locus_tag="L3M60_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CGNR zinc finger domain-containing protein"
                     /protein_id="HBN6226960.1"
                     /translation="MNKKIELIGNFASDWIRYSDYEYRITEEDELYIVPTEDAVFSMY
                     NPFDVADDIIVDIIRIGQEALQDISKETKEMKLKKEVLDFVKKYGLLGLIYSSVYNRD
                     IVGEERVLMIEKNYITKENILSADEYVNMFIPFASDEDVYFKKYRRGIDVSKREESPK
                     FFGKRPLILDLIFSKFYTEQLDWIVSFAKDMYKHFNQICVYKNTSNLLTESVTIMSGG
                     FNPQKIGFSISQLDKPIIAWEFDSLKTAIETIYAFAITSEPSSLNKCKYCGKIYIPTN
                     SKSQYCSPSCRNCSNVKKSRSRAKKDK"
     gene            15419..15853
                     /locus_tag="L3M60_002302"
     CDS             15419..15853
                     /locus_tag="L3M60_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226961.1"
                     /translation="MISPIILSSINRNLKEIERNKLFETNIESRDYGLTLSESDVKDI
                     INFRDNTLKGYGRIELDIKVTKQLIENIYISQYTNMDNYLETINDMQEIFYYLKNETD
                     DKICDDEIIEILDELYEKFSGNINNVRGEADEFVKKFKFGEV"
     gene            15876..>16115
                     /locus_tag="L3M60_002303"
     CDS             15876..>16115
                     /locus_tag="L3M60_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226962.1"
                     /translation="MSNNLSNININENNLIKNQYSISLIKECFDCKVIDEREVYNIQQ
                     EISLILMDLIKKYTNGQSTSVKTEVAEKLLISIWYA"
BASE COUNT         6460 a         1577 c         2948 g         5130 t
ORIGIN      
        1 atatggctta aaaatatata aagatacttt aaaatgaaat gtaacttaaa ttaaatttta
       61 gtaggaattt tataggttta tttttaaagg ttttttgaaa ataaatctat ttaaaaatta
      121 aattaaaatg caagaaaaac ataataactt tcatttttaa gaagttttca caaaaaagaa
      181 aaaatatagt gcaataaatg atacaaagta atataataga ttacaagata tgaaaaacta
      241 aaaaaataaa ttgcaaatga aattaaaatg aataaaaaat aaaaaaataa tcataagtta
      301 gaggtatcaa tggaaaataa gacctaatgt ttttaaaatt aaaagtatac taaggggggc
      361 attaatatgt gcgttatatc aaaagataga gcaaaggaaa taaaaatagt agatactact
      421 ttaagagacg gagagcaaac agctggggtt gtatttgcaa atagagaaaa aataatgatt
      481 gctgaaatgt taagtgacct aggtgttgac caaattgaag ttggtatacc tactatgggt
      541 ggagatgaaa aaaatgtaat aaagcacata tgtagtagaa atttaaaatc agatataatg
      601 gcttggaata gagcagtaat aaaagatgtt gaagaatcta tttcttgtgg tgttgatgca
      661 gtagctatat caatatcagt atctgatatt catatagaaa ataaattgag aacttctaga
      721 ggctgggttt tagaaaatat ggctaaaaca gttgaatttg caaagaaaaa tggactatat
      781 gtttcagtaa atggagaaga tgcttcaaga gcagatatag acttcttaac tgaatttata
      841 aatgttggaa aacaagcagg ggcagataga tttagatatt gtgatacagt aggagttatg
      901 aatccttttt ctataaagaa tgcaatagaa actctttatg aaagaactaa ttttgatata
      961 gaaatgcata cacataatga ctttgggatg gcaactgcaa atgcacttgc aggaatagca
     1021 gcaggagcaa attacgttgg tgttacagta aatggattag gtgaaagagc aggaaatgca
     1081 gcattagaag aagttttaat ggcacttaag tgtgtttata aatgcgattt aaataatata
     1141 gatactagaa aatttagagg aatatgtgag tatgtagcac aggcatcagg aagaatcctt
     1201 cctacatgga aaccagtagt tggggataat atgtttatac atgaatctgg tatacatgca
     1261 gatggagctt taaaagaccc acataactat gaaccatttg acccaagtga agtaaatctt
     1321 gaaagaaaaa tagtaatagg taaacattca ggaagagctg cagttgtaaa taagttgagt
     1381 gaatatgaaa tgtatatttc tccagaaaac gctaccaaac tattaaatgc tataagagct
     1441 acatctataa gattaaaaag aagtttaatg gataaagaaa ttcttcaatt atattgtgat
     1501 atactagcac atgagaaggg tacaacagaa gaagaagcag ttagaggttc atatatttaa
     1561 gatataattg tttaggagga aaatatggga gataatatag tttataaaat aattaaaaaa
     1621 catatagtag atggagaagc tgttgctgga agttcaattg gtataaaaat agaccaaact
     1681 ttaacacaag attccactgg aacaatgacg tatcttcaac tagaagctat ggggatagac
     1741 aaagtaaaga ctaaaagaag tgtggcgttt gtagaccata atatgttaca acaaggattt
     1801 gaaaatgcag atgaccataa gtatatacaa acagtggcag ataaatatgg agtttatttc
     1861 tctaagccag gaaatggtat ttgtcatcaa gtatttttag aaagattttc aacaccagga
     1921 gatacacttt taggttctga tagccacaca cctactgctg gaggtgtggg aatgatggct
     1981 attggagctg gtgggttaga tgttgcttta gctatggcag gaggagcata ctatataaaa
     2041 gcaccaaaag tctgtaaggt gaatctagtt ggaaaattaa ataatatggt atcatcaaaa
     2101 gacataatat tagaagtatt aagaaaacaa actgtaaaag gtggagtagg taaagtttat
     2161 gaatatggcg gagaaggtgt taaaagccta tctgttccac agagagctac aataacaaat
     2221 atgggggctg aacttggagc aactacttca atcttcccaa gtgatgaaaa aacactagaa
     2281 ttttttaaga gccaaggaag agaagatgca tggatagagt taaaaccaga tgcagatgca
     2341 gtttatgatg aagagattac tataaacctg gatgaattaa aaccgttagc tgcaaaacct
     2401 catagtccag acaatgtaga tgaagttgaa aatataggca aaattaaaat agaccaagtt
     2461 gcaataggaa gttgtacaaa ttcttcttat gaagacttaa tgaaagtagc acaaatccta
     2521 aaaggaaata aagtacataa ggatgtgagt ttggtaatag caccaggttc aagacaagtg
     2581 atggagatga ttgctagaaa tggagcttta gcagatataa taagtgctgg agctagaata
     2641 ctagaaaatt cttgtggtcc atgtatcggt atgggtcaat ctccaggtac tgactctgtg
     2701 tctttaagaa ctttcaatag aaatttctat ggtagaagtg gtactctatc agcgcaagta
     2761 tacttagtaa gtccagaagt agcagcagta agtgcaatca agggagtgct tacagaccca
     2821 agagaatttg atattaaatt taccaattta gatgtaaatg aatttttgat agatgatagc
     2881 atgataatta aacctgctga tgtaggaagt gatgtagaag tagttagagg acctaatata
     2941 aaacctttcc ctcttaatac agagctaagt cagtctatag atggaaaagt tatattaaag
     3001 acagaagata atataactac tgaccatata atgccatcaa atgctaagtt acttcctttt
     3061 agaagtaata taccgtatct tgcaaactat tgtttcaaca ctgtagatac agaatttcct
     3121 cagagagcaa aagagaataa tggtggattt atagttggtg gagataatta cggtcaaggt
     3181 tcaagtagag agcatgcagc tttagcacca ttatatctag gtgttaaagg agtaatagtt
     3241 aagagctttg caagaatcca caaggctaac ttgataaata gtggaataat tccaatggag
     3301 ttttgtgatg agaaagacta tgaaaatata tctcttttag acaatttaga gataccaaat
     3361 attttagaca atctaagtaa tggaatttta gaagttaaga atacaactaa aggaacttct
     3421 tttaaggtta aagtagaatt atcagcaaaa gaagttgatg tattaaaagc tggtggaaaa
     3481 ttaaactata ctaaaaatca agctaattaa tatgaaaaga gggaatattg aatgtataaa
     3541 gtgacactta taccaggaga tggaatagga ccagaagttg cgaaagctat gaaaaaggtg
     3601 gtagaagcta ctggagtaga gatagaatgg gaagaagtta atgcaggaga ggcagttatt
     3661 gaagagtatg gcactccact tcctgaatat attatagaca gtataaaaaa gaataaaata
     3721 gctataaaag gtccaattac tactcctgta ggtaaaggat ttagaagtgt aaatgtaact
     3781 ttaagacagg cattagattt atatgtcaat ttaagaccta ttaaaagttt taagggtata
     3841 aaatcaagat atgaagatgt tgatttagta gtggtaagag aaaatacaga agatttatat
     3901 gctggaatag aacataaaat aggtgattat gcagctgaaa gtataaagat aataacaaga
     3961 agtgcaagtg aaagaatcgt tgattttgcg tgtaactatg ttaaagataa caagagaaaa
     4021 aaagttacag ctatacataa agcaaatata atgaaaatgt ctgatggatt atttttagat
     4081 gtgtttagag aagtagcatc aaaacatgat gtagaatatg atgatttgat tgtagatgca
     4141 gcagctatga acttagtttt aaatccagaa aattatgatg taatggtaat gccaaatctt
     4201 tatggagata tactatcaga tttaggtgct gggcttgttg gaggtttagg tataattcca
     4261 agtgcaaata ttggtaaaga ttgtgcgata tttgaagctg ttcatggaag tgctccacaa
     4321 atagcaggtc aaaataaagc taatccaaca gcacttattc agtcatcagt aatgatgctt
     4381 agatacttag gcgaatatga aaatgcacaa aaaatagaaa cagctttaga aaaagtattt
     4441 cttgaaggaa gcaaattgac tgtagattta ggtggctcag cttcgactac agagtttgca
     4501 gatgaggtgt gtaagtatat tttatagatt ttgtttatgg aaaaagtaga atatagatta
     4561 aaagaaaggg gctttctcaa aatagagaga agcctctttc tttgttaaaa tgataaaatg
     4621 tatttgattc tatgcaaaag agagttcctc ataaacgaaa aagttcattt tgggatgctc
     4681 actttaattt taaaattatt tgattaatag gcaaataaag gttaaataaa tttagtgttt
     4741 ttaattgaac tttcaaccaa tatatggtaa aatttaaaaa agttgaactt tcaactaaaa
     4801 acaaatatat tgattatgtg aaattctatg aataaggaac tctgtgactt tagtcatgtt
     4861 aggttcagtg gaggtgtgtt tgattaagac cttagatagt aatatattaa gagaagtagg
     4921 tactttatct agagcagtaa attcaataaa tgatattaag tacaaggaat taaagttaca
     4981 aaaaggacaa tttacattct tgacaagaat ttgtgaaaat cctggtataa accttgtgga
     5041 attatcaaac attttaaagg tagacaaggc tacaacaacc aaagctatac aaaagttaat
     5101 aaaagctgga tatgtggata aaaagcagga tgagtttgat aaaagagggt ataatttaac
     5161 tccaactaat aaatctttgg aagtttatga gttgataatt gaagaagaga atagaagtat
     5221 agagatatgt tttaagaatt ttacagatga agaaaaacaa gtggtaacta aattattaga
     5281 aaaaatgagc aaaaatgcag aaaatgaatg gtttaaagta aagagatagt aaataaatac
     5341 attaacctaa ggagatagtt ttatgattag aaaagccaat atgaatgatt tagaaagtat
     5401 tatgaaaatt ataaaatcga cagtgaaaga aatgaagact tataataata cacaatggga
     5461 tgaaaactat ccattagaaa aagactttgt ttcagatatt aagaaacagg acttatatat
     5521 atatgaagtg gatggagaag tagcaggatt tatatgtctc aattatgaag agccagaaga
     5581 atatctaggc ttaaattggt catctaataa aaaagcaatg gtaatacata gaatggctgt
     5641 aaatccaaat tttagaaaga aaggaatagc atctaagtta gttgattttg ctgaaaaatt
     5701 agctagagaa aacaatgtat tatacttaaa gagcgatacg tattcaataa attcaaaaat
     5761 gaatttatta cttacaaagt gtggttttat aaaaatcgga gaaatgagtt ttttaggaaa
     5821 ggagaaatct ttctactgtt atgataaaat attataaaaa gcatttataa tacaaggggt
     5881 aaataataaa tttttggtat tagtaagggt ataatataac ctagtgtatg ccattatgaa
     5941 agggatgatt tatgtgattg aatttttaaa gaagagaaga agtattagaa aatacaagga
     6001 tattgaagta gaaaaagaaa aattagataa aatattaaaa gctgctcttt tagctccatc
     6061 atcaaagggg cttagaacat gggaatttat agtggtagat gataaagaaa agttaattaa
     6121 attatctcaa tgtagaacta aaggtggcgg atttttctta aaaaatgcac ctttagctat
     6181 tgcaattatt gcagataaag aaaaaaatga tgtttggata gaggatgcaa gtatagctgc
     6241 ttcatacata caacttcaag ctcatgaact aggattaggt tcttgttgga tacaagtaag
     6301 aaatagaatg catgatgata atatagaagc tgacaaatat attagagaag aattaaaagt
     6361 acctgacaag tatagtgtag aatgtataat ttctatagga tatcccgatg aagaaaagaa
     6421 agcatacagt gactctgatt tagactatga aaaagttcat tttaataact tttagtatat
     6481 aatatgttgt attaagacat tatatttttt tatattgtta atatatactt aaaattgtaa
     6541 aaatatatct gaattttact aacttatcta tgggttatgt acaaggattt taaagataaa
     6601 tatagaaatt aatgtttata atcgatacat aacttgagta acaaaattta aggaggttaa
     6661 agtatgggta tatttaaaac aaaaattgat gaggactgga aagtaaacta cattaaagag
     6721 tttaatgaaa tgagagattc ttatgagagt aaacttcaaa aaaaacagtt tgaagtagat
     6781 agtttgaaat cagaactgga tagactaaga agctataaaa atagccttaa gccaaaagaa
     6841 aaacaaatta ctgatgatga tataaataat ataaagaatt taagaagaga tggtttaagt
     6901 tataaagaaa tttcaaatca aacaagttgg agcaaggcaa ctgtaagtag agtattaaat
     6961 ggactttacg attagcatat acatggggtg atttaaattg ggatgaagta tagttacaaa
     7021 aaatgtataa tagattctat tttattgatg cttatagttc agctattaag gatgattttg
     7081 aattatgtac ttctaagtca atttgaattt actcttgaaa attttaatat tataaactta
     7141 atttctttta cactagttgg attatcatta attctatttt taaaagataa ttctttgtat
     7201 aacaaggtga gaaatagaaa aatcacagaa gcttttgaag aaaataaaaa cagtatactt
     7261 attgaaaagt gtaagctgat actttttgtt gtggttttat ctttagcaat aatttcaact
     7321 tattgtacta agggatatgt attttttaat gtaactatga tgactttgtc tgtatttata
     7381 gttccaatat ttgaagagtt attttttaga gaatacatat ggaattatct aagtaatttt
     7441 ataaagtcaa agattaaaat tatatgtata acatctatat tatctggaat ttataatata
     7501 ggatatatag atattataag aaattatata gcattgtata ataattcaag ctacaccttt
     7561 gaagttatta tttcaaaaat tattataggt actgtttttg gtattgtact tggattagtt
     7621 agatataggt ttagagatgt aaggctttgt atactattga ggagcttatt tgctatattt
     7681 attaggtaga taatataatt agtttataag gttgttttaa atgtaatttt aaatcaacct
     7741 tattttattt gtgttctata aagtacttat gtagtaataa ctaatgaatt taataaaaat
     7801 tatttgaata tttttaaaat atataaatat gtattgacat ataaatatta tcaattacaa
     7861 tttatataaa ctagtaaaaa ttaaataata ttaatcaact tattgaatgg aggaataaaa
     7921 atgaaatttg tgactttttg tagtagcaat gaagaaaaaa taggtgtatt taattcagaa
     7981 acaaatagta tatatgaaat taatagttta ggattaagta aattatatac agatatgaat
     8041 gattttattg aaaacgtatc tacaggagat ttagaaaaaa taaaaaacaa ttcatttgaa
     8101 aatgcaaagt gttataagtt agaagaagtt aaattatgtt ccccaattgt aagacctaaa
     8161 aaagatatta tatgtttagg tctaaactat aaagaccatg tcaatgaaat cccagatgga
     8221 gttataaaaa acgtagttat gccagattat ccaatttatt tttctaagag agcagataaa
     8281 ataattggag tagatgataa gataagttta catggagact tagtagaaaa gctggattat
     8341 gaatcagaac ttgcagtaat cataggtaaa gaaggtataa atatatcaaa agaagatgct
     8401 tatgactata tatttggata tacaatagtg aatgatataa gtgaaagagc actccaagat
     8461 aaacatgttc aatggtttag aggaaaaagc ttagacacac atacatctat gggaccttgc
     8521 atagtacata aagaagaatt tgaacagcct ttaaaattag atataagtag tgtagtaaat
     8581 ggagaagtaa gacaaaattc taatacagaa tactttatat ttgatatacc taccataata
     8641 aatgatttat caagaggaat gactttaaaa ccaggagata taatttcaac tggaactcca
     8701 gcaggtgttg ctatgggtat gaatcctcaa gtatatttaa agcatggtga tgtagttgaa
     8761 tgtaaagtcg aaggaatagg tgttttaaaa aatatagttg attaatggat aaatatgcca
     8821 tataatttta ttagtacgaa taatttaatt aatataggat taatcaaact tatgattaat
     8881 ttattataga gagggatgag tatatggcaa gagaactaga attaaagtat ggctgtaatc
     8941 caaatcaaaa accatcaaag atttatatga agaatggaga gcttcctata gaagtattaa
     9001 atggaaaacc tggatatata aactttttag atgcatttaa tagttggcaa cttgtgaaag
     9061 agttaaaaga agctacaggt cttccagcag caacttcatt taagcatgta agtccagcag
     9121 gagccgcagt aggaatacct ttatcagata ctttaaagca aatatatttt gttgatgatt
     9181 tagagctttc tccattagct tgtgcttatg caatggcaag aggagcagat agaatgtctt
     9241 cttatggtga ttttatagca ctttcagatg tatgtgataa agagactgca actattatag
     9301 caagagaggt ttctgatggt ataatagctc caggatatac agatgaagcc cttgaaatat
     9361 taaaaggaaa gcgtaaaggt aactacaata ttgtaaaaat agatgaaaac tatactcctg
     9421 aacctattga aacaaaggat gtttatggaa ttacttttga acaaggcaga aatgaaatat
     9481 taataaatga agacctatta aaagatattc caactgataa taaaattttt acagataatg
     9541 caaaacgtga tttaattata gctcttatta cattaaaata tacacaatca aattctgttt
     9601 gttatgctaa agatggacaa gtaataggag taggcgcagg tcaacaatca cgtattcatt
     9661 gtactagact tgcaggtaat aaagcagata cttggtatct tcgtcaacat cctaaagtat
     9721 taaacttaaa attcaaaaag gatataggtc gtccagatag agacaacact attgatgtat
     9781 atctttctga tgattatatg gatgtgcttg ctgatggcat atggcaaaac ttttttgaag
     9841 aaaaaccaga gcctctaact ggagaaggaa aaagagcatg gcttaaaact ttaacaggag
     9901 tagcacttgg ttcagatgca tttttcccat ttggtgataa tatagagcgt gcaaaaagga
     9961 gtggggtaag ctttatagca caaccaggtg gttctattcg tgatgataat gttatattaa
    10021 cttgtaataa gtataacata gtcatggcat ttactaaaaa tagattattc catcattaaa
    10081 atactgaaaa tataaagata atatagatta agtaaaaatc tacaacaagt tttgttgtag
    10141 atttttttat ttgctgtata attttaaata gaactttatt tttagatatt caatttggag
    10201 gttgatatgg ctttaaatat aagaaactat agcctattat gtaatcaagg gacagctaaa
    10261 tttaatgaag atgtagtcgg tataaatcca tttggagcat ggattttaga tggtgctaca
    10321 ggacttaatg gaaaaaattt agtgtcaact gaaagcgatg caaggtggta tgttcagtgg
    10381 tggaataggt atttatataa aaatataaat aaagaagaat cattaaagag tattattaat
    10441 gctggaataa tagaagtaaa aaaagagtat gaactaagat taaatggtat taaggttgaa
    10501 aaattagatt taccatctag ttctatagct gttgtaaaat ttcatgaaaa caaagtagag
    10561 tattttttat taggagattg tactttgttt attaaaaatg gagagtccaa agttataaaa
    10621 gatagaagta tttgcaaatt tgataatatt gtatttgatg aaatgagcaa actttctaac
    10681 ttggaagaga tggcatttga tgagattaga actagtgtta tggatacaat agtaagtaat
    10741 aggttgaaga aaaatacgaa agatggatat tggatattgg attttgatga ggaagctgtg
    10801 gaaaattcta tatatggata cattgaaata aaaaaagatt ttcaattgat gctaacaagt
    10861 gatggtttta caagtgcatg tgatagatat aatctaataa aagaagaaga attgatacgt
    10921 atagcagaaa aatttggtat agggtatata cacaataaag taagagattt tgaagataat
    10981 gactataaag cggttaaaat cccaaggttt aaagtaaaag atgattcatc ttgtttatac
    11041 ttagatatat ataatgatta gactgtgtaa aactatttaa ttttagaagg gaggtaagtg
    11101 aaagtgaaaa ttcttcatat aataacacaa aaaccaaata gtacaggtag tggtatatat
    11161 ttgtctggta tgataaaagg attcgaaaag ataggtcata agcaagctgt aattgcaggt
    11221 atagatgtaa atgatgatgt aaattgtttt ccaagtgatg taagttttta tccagtaaag
    11281 tataattgtg gggagttaaa ttttcctgtt gtaggtatga gtgattctat gccatatgaa
    11341 agtacaagat ataaagattt aaatatagac atgataaata aattgaagta tcaatttaaa
    11401 ataaatatag atagggctat gaatgatttt aaaccagaac ttataatatg tcatcatttg
    11461 tacctattaa ctgcatttgt tagagaaaca gtaaaagaca taaaggtcat gtctatttgt
    11521 catggaactt gtttaagaca gctaaatacc attgatttgg agaaagaata cataatatca
    11581 aatataagaa aattagattt aatatttgct cttcatgaaa atcagaaata tgatattata
    11641 aagacttttg gagtaagtga aagtaaagta gttgtaattg gtagtggtta taatgatgat
    11701 atattttata ataaaaatta taaaataaag gatgataaaa taaaaatagt atttgcaggt
    11761 aagatatgta aatcaaaggg actaattcca tttataaaag ctatatctaa attaaaatat
    11821 agtagagatt taatagaagt caattttgca ggtactggaa gtgatataga atcatataat
    11881 gagatagtta aactcgcaag taagtctcca tttaaaatga atttcctagg aaagttagag
    11941 cagagagatt tagctgagct attcaacagg tcacaaatat ttgtgttgcc atcattttat
    12001 gagggattac cagttgtagt tttagaagcc ttatcttgtg gaacagatgt aatcactact
    12061 gatattcttg gtgttaaaga gtggattggt agtgagatta ataattctgg aaagatagaa
    12121 tatgtttctc taccttttat ggagaaagaa ggtattccaa aggatgaaga gttacatgac
    12181 tttgaaaata atttgcataa tgcaatagat tctaagatac aatctttatt acataactca
    12241 aataaaaaaa catctgtaga tatgtcaaaa aaaacgtggg atggcttagc ctatagaata
    12301 aatgaagtta ttttaatgga tgagttgtgt ttggtttaaa tttatttaga ttataaaaaa
    12361 gtgtaactat ttttacactt tttttaaatg attttatatt atactaatac taaatatatt
    12421 tgtttttagg gaggtaattt atgaggatta gtaaaaaggt attggcgtta ggtatttcag
    12481 cattattgtt aagtgttagt tttccaacat caataaatgc actagataag attgaaaata
    12541 ttaaaggagt agataaatat gagacagctg ggcttatttc agataagcaa gattttacaa
    12601 cagcagttct tatcaacgcg gattctacaa tggtagatgg tatcgctgcc agtggtcttg
    12661 caggagtaaa taatgcagct attttattaa ctaataagga tgatatacca gaagcaacac
    12721 ttcaaagact gaatagagtt actaagatat atgtaatagg tggagaaaat tctataagta
    12781 aagatgtaga agaaatgtta cttatgagaa gaatgcaagt tataagaatt gatggtgtag
    12841 atagagtaga tacaagttat aaaattgcag gtgaaataga aaaaattaaa aacagcgata
    12901 agatgttttt gataaatgga tttaaggatg aagctgatgc agtaagtgtt gcatctgttg
    12961 cttatagaga tggagctcct ataatattga caaaagatat accttcagca gaagaagata
    13021 cagacttaga cccatggttt ggagtttctc ctgttccagt atatgctatt ggaggagagt
    13081 ctacactaag tgattacata gtgagtagat atagagcaac tagaattggt ggtgttgata
    13141 gatatcaaac taataaaaat attattgaaa agttctataa tggagcaaaa gagttctata
    13201 taacaagtgg agacgattta gtttatgctt tggttgcatc accacttgca aaaaatgctc
    13261 ctgttgcttt agtttcaaat aaaagtgata agagtatatt aagtggagct agtaaagtaa
    13321 cagcaatagg tataagtgat aatagtatta tagagcagtg tttggatgct gtaaaaaagt
    13381 aatagagtgt cattagtaaa atgaatatat accattgaaa attagtagat agttcatttt
    13441 ataatggtat aaaatatatg agaagaggct atcaaataga gataagcctc tttttcattg
    13501 ttaaaattat gaaaatatat ttatttcata aaaaagtatc acatgaacta aatgtctatt
    13561 ttgagacact atcttatttt cttagattag atgatttttt atatggaata aatttcataa
    13621 gtattatctt ccttcaattt ttccaaccaa accatctcta aaatatctag ttcttctaaa
    13681 tagtctttct ttgttttgtt atcatcatgt tttataagtt ctaaaacttc aaatgtaaat
    13741 gcattttcac catatttttt ccactcatta tttaactctt ttatcataca tgaaccagca
    13801 ccaagctgaa atttgataga gttaaatttg ctttttatgt cattactttt tccaaggtaa
    13861 atctttttag taatatcatt ttttattatg aatacacctg tgtcaaattt cctttctttg
    13921 tataattgtt ttaattcacg ttttctatcc atgattttat atacctctct ttataattaa
    13981 tatgtaatat gtaaatataa aaatatatta catactacat tataaatata ttaattttat
    14041 atgtaaatag tttttataaa tttaattatg gactagttat taataaacaa gaaatttagc
    14101 ttagtaagtt cattataaag gttgttataa attttgatat tacagaaata taaaagaata
    14161 gttgacagta tagaaaatat ggaataaact aaaaatgtaa tagtaatata aaattatata
    14221 ttacattgaa aggggtaaat gattatcatg aataaaaaaa tagaattaat agggaatttt
    14281 gcaagtgatt ggattaggta tagtgattat gaatatagaa ttactgaaga agatgaacta
    14341 tatatagttc caacagaaga tgctgtgttt agcatgtaca acccatttga tgtagcagat
    14401 gatataatag tagatataat tcgtatagga caagaggcac tacaagatat atcaaaagag
    14461 acgaaagaaa tgaaattaaa gaaagaagta cttgactttg taaaaaaata tggattattg
    14521 ggtttaatat actcaagtgt atataataga gatatagttg gagaagaaag agtactgatg
    14581 attgaaaaga attatattac aaaagaaaat atattgagtg ctgatgaata tgtaaacatg
    14641 tttataccat ttgcaagtga tgaagacgtg tattttaaaa agtatagaag aggtatagat
    14701 gtatcaaaga gagaagaatc tccaaagttt tttggaaaga gaccattgat tttagattta
    14761 atattttcaa agttctacac tgaacaacta gattggattg taagctttgc taaagatatg
    14821 tataagcact ttaatcaaat ttgtgtatat aaaaatacct ctaatctgtt aactgaaagt
    14881 gttactatta tgtctggagg gtttaatccc caaaagatag gattttctat aagtcaatta
    14941 gataaaccaa ttattgcatg ggagtttgat tctttgaaaa cagcgattga gactatctat
    15001 gcttttgcaa ttacaagtga accttcttct ttaaataaat gtaagtactg tggtaaaatt
    15061 tatattccta caaattctaa atctcaatac tgttcacctt cttgtagaaa ttgttcaaat
    15121 gtaaagaaaa gtagaagtag agctaaaaaa gataaataaa attaataaat atatcattgt
    15181 ttagatttta gtaaataact gctgaattaa aagttgttat agaaaattat atatctaaat
    15241 aatggtattt ttttattgag aaaatggaaa agtttaattg agatagtaat tttgagttga
    15301 caaaaactac ttacaataat actattataa gtagttgcta aaagaatgta tattaattta
    15361 aatataacat aaaaaataaa aaaagtcaat agaaaaaata aaaaaggaga gtttaaaaat
    15421 gatatcacct attattttga gttctataaa tcggaattta aaggaaatag aaagaaataa
    15481 actgtttgag actaatatag aatctagaga ttatggtcta actctttctg aatctgatgt
    15541 aaaagatata ataaatttta gagataatac tttaaagggg tatggaagaa tagaattaga
    15601 tataaaagtt acaaaacagt taatagaaaa tatatatata tcacagtata caaatatgga
    15661 caattattta gagactatta atgatatgca agaaattttt tattatttaa aaaatgaaac
    15721 tgatgataag atatgtgatg atgagattat agaaatactg gatgaacttt atgaaaagtt
    15781 ttcaggcaat ataaacaatg taagaggaga agcagatgaa tttgtaaaga aatttaaatt
    15841 tggagaagtt taatgtatga tgggagagat ttgatatgtc aaataatcta agtaatatta
    15901 atataaacga aaataattta ataaagaatc agtattccat atctttaatt aaggaatgtt
    15961 ttgattgtaa agttatagat gaaagagaag tatataatat acaacaagaa atatccctaa
    16021 tacttatgga tttgattaaa aaatatacaa atggtcaaag cacatcagta aaaacagaag
    16081 ttgctgaaaa attgttgatt tcgatatggt atgct
//
LOCUS       DAFNNB010000088        16097 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0089, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000088 DAFNNB010000000
VERSION     DAFNNB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 16097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16097
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0089"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            26..472
                     /locus_tag="L3M60_002304"
     CDS             26..472
                     /locus_tag="L3M60_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6226963.1"
                     /translation="MDFKNLQYESLSYIICNLQKNFKLYCEKCLKPYKLTNGLYFYLI
                     YINKNRNCSLNDVSTEFEVDKAHTTRTISRLEQDGYIEKIQNPNDSRAFQLRVTDKGE
                     EVLGDIKNIFSKWDNRIKKEFSDEEYKELVKKLHVVKDIKTAMEEE"
     gene            474..1568
                     /locus_tag="L3M60_002305"
     CDS             474..1568
                     /locus_tag="L3M60_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HBN6226964.1"
                     /translation="MYCNLLKPIKIGNLELKNRVSFAPTSMGLKLEEKIKKFCDIAKS
                     GVALITLGDVSVRPSFHNMAMSLTDEDGVMKYKKIVDEIHNSGAKVSAQIFCSDYDVN
                     LIKDTMKMGITSHVEIKKIMNDGVKDYITNMPKEEIKNIINLFKVTALNAKKAGFDMI
                     QIHGDRLIGSFSSSIFNNRNDEYGGTCENRSRFASEIISSIRNEVKDIPIDYKFAIRQ
                     ENPHYGNAGVLFSEVEYFVKKFESLGVNSFHVTLANHSKLEDTIPINNHPYFKDEGCF
                     LYLADEVKKHTNLPVCGVGKLSSPDFIESIISNNRVDMISMSRQLLADSNWLQKVKDG
                     RVDEIKKCCYCNKKCADALQTHSQFGCILD"
     gene            1650..2180
                     /locus_tag="L3M60_002306"
     CDS             1650..2180
                     /locus_tag="L3M60_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBN6226965.1"
                     /translation="MKSLILYSSLTGNTKKIACSIYEAIQGKKEIKNISELVDDSIDY
                     ENYDIVFLGYWVDKGICDKNSKQVLENIHNKKIALFGTMGASTKGSYGASIIEKIESI
                     IPKDNEILGSFICQGKIAEGLKVKYKEMLKLSPDNEHIRQQLNNHEESQSHPDEQEIY
                     EANVFAKNMMIKASTM"
     gene            2507..3340
                     /locus_tag="L3M60_002307"
     CDS             2507..3340
                     /locus_tag="L3M60_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6226966.1"
                     /translation="MTIKEVEEKTGLTRSNIRFYENENLIKPLRNERNGYREYSDENI
                     EDIKKIAYLRTLGITIDDIRNIILQKVSLVEVVEKQEKVLETKISDLENAKTMCNKML
                     ESDELSYLNLKVEQYITELPEYWNKNKPVFKLDSVSFLYIWGRKITWGIITIACLLIA
                     IIVFPKLPHQIPIQWNNGVANYVDKKFIYAYPAICVVFRILLRPFIWRWLQIHAFYSD
                     TIADYLTNFMCFVALSIEIFSILFIYNIARHVTIIIFIDIFVFIALLIIGVYKLFKKY
                     N"
     gene            complement(3590..4798)
                     /locus_tag="L3M60_002308"
                     /pseudo
     CDS             complement(3590..4798)
                     /locus_tag="L3M60_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436150.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4987..6099)
                     /locus_tag="L3M60_002309"
     CDS             complement(4987..6099)
                     /locus_tag="L3M60_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBN6226967.1"
                     /translation="MKISEVSTPNFLLDLDQLEKNIDKIQQICTNNNKQLWPMLKTHK
                     STYIARLQKNAGASGFLVGTLDEGEALIKAGFEDIMLAYPYMGEVNINRIVTMASKIN
                     LICATDNIECAKAYSEAFSKSKLECKLLIIVDCGLHRFGVEPEKVVELATEISKLENI
                     KIVGISSHPGQVYGCSSPEGVPDVCVQEDSSMEKAYKALTDNGFNIEIVASGSTPTVE
                     AEAVSKTITAVRPGNYVYYDAIQIGLGCATEDMCALTVVCTVISKNSSGYYLIDCGSK
                     CLGLDKGAHGNSSIVGYGRVCGHPELTIDSLSEEVGKIKVNGETDIKIGDRIRIIPNH
                     SCSTANLTSNLLGFRGDTIERVIEVDIRENSKQIIL"
     gene            6397..6978
                     /locus_tag="L3M60_002310"
     CDS             6397..6978
                     /locus_tag="L3M60_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBN6226968.1"
                     /translation="MLEVVNMRGVVNMRKVILYIATSLDGYIAEEDGSVSFLDEISSV
                     FKNEQEDDYNTFYDTIDTIIMGNSTYVQIANELSPNEWYYKGKECYVYSNIINEITEN
                     VKYTNLEPKKLIEQIGREKDKKDIWIVGGGDIVKLFMKDNLIDDYYIYILPIILGRGI
                     SLFKSGIDKTNLNFKKASNIGELVKLEYSKKQK"
     gene            7223..8950
                     /locus_tag="L3M60_002311"
     CDS             7223..8950
                     /locus_tag="L3M60_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003496244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226969.1"
                     /translation="MKLILSYLKNYKLLIVLNILAIFSFALVELGIPTIIAKIIDNGI
                     ANQNIAYIKQMGIVIVIISIIGVVGSVLLGYCSAKVSTSVTRDIRNDIFEKSQEFSHT
                     EYNKFGISSMITRTTNDAFQIQQFVNILLRTALLTPVMFIISIIMTIRTSVELSIVLA
                     ISVPFIIIGVAIIAKVSQAISSKQQKGLDKLNLISRENLTGIRVIRAFGNDDYETERF
                     EKTNTYYANVSKKLFKLMSITQPAFFLLLNIAVLAVFWISSEKINIGELQVGQLVAFL
                     EYLFHAMFSIMLFSMVFIMYPRAEVSANRIKELLNEEPLIKNPENGIKENENKGIIEF
                     DNVTFTYPDGEASVLKDISFTAKTGETVAFIGSTGSGKSTLINLIPRFYDVTEGSIKI
                     NGVDIREYDLKALRKKIGFIPQKSLLFTGSIANNIRFGKHKAGESELEYSAKVAQAYE
                     FISKKPRKFDELISEGGANVSGGQKQRLSIARAIIRRPEIYIFDDSFSALDFKTDAIL
                     RAKLKKETKDAIVLIVAQRISSIIDADKIIVLNEGQVVGMGTHKELLKNCEIYYEIAT
                     SQLKKEELE"
     gene            8950..10740
                     /locus_tag="L3M60_002312"
     CDS             8950..10740
                     /locus_tag="L3M60_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6226970.1"
                     /translation="MKKSKSFKKTIGRLLPFVKKYKFSFIIAIICIISAATMNALAPK
                     TEGLIITQLTKDVISIAKGIPGASVNFDYVTKILVILACIYVANAIFTYTSSFLLTNA
                     IQNTMRDLRNEVENKIRRLPISYFDSNSFGDVLSRISNDVDTISNALQQSFMQIVNSI
                     LVIILALSMMFTINIYMALIALFIIPISYFVSKFVVKKSQNRFSLQQNALGKLNGKVQ
                     EMYTGFNEIKLYGKEEDSIKEFKKVNQELCENGFKAQFISSMMNPMVSLVTYFGIAAV
                     AVVGSIYAVSGGITVGNLQAFVRYIWQINQPLSQMTQLSTVIQSSFAAIERVFEILDE
                     EEEIPDVENPVKIENVKGNVTFEHVNFGYGENSTLIEDLNAEVKSGQMVAIVGPTGAG
                     KTTLINLLMRFYDVKKGAIKIDGVDIRDMKRKDLRSMFGMVLQDTWLFNGTIFENIEY
                     GRFGATKEEIIQAAKVANVHHFITTLPDGYNMFLNEEASNISLGEKQLLTIARAFISD
                     PSILILDEATSSVDTRLELMLQKAMRNLMNGRTSFVIAHRLSTIRNADLILVMNNGSI
                     IEQGNHDELMEKGGFYEKLYNSQFADTESE"
     gene            complement(10901..11209)
                     /locus_tag="L3M60_002313"
     CDS             complement(10901..11209)
                     /locus_tag="L3M60_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226971.1"
                     /translation="MFNKVYEKKEFIVFQVKKGYVVYNTRKSFEEGHTHLKHFEAAKT
                     AIDLAINKKIPRSKDGYYLTSLIRISDDGYYIDKLSELLYVREQKGKKEKYCNSGYQM
                     "
     gene            complement(11471..12019)
                     /locus_tag="L3M60_002314"
     CDS             complement(11471..12019)
                     /locus_tag="L3M60_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226972.1"
                     /translation="MIFLNEDVVTNETIDKDYAIEEVRMACKHFGDLYFYFSKVLFEE
                     FGEDKTSEILKKVLFERSEERAIAMRERAHENGDELIADNIISTTDVPFLGWVPEFEE
                     LHCPYGASWLLRFEENPWFKKFASLYCDVTDTTVAEVFTGDTSHKITKNILWGDKSCE
                     RIYFHDDKVSEGKYTYGNKNCK"
     gene            complement(12121..12792)
                     /locus_tag="L3M60_002315"
     CDS             complement(12121..12792)
                     /locus_tag="L3M60_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6226973.1"
                     /translation="MATFKRLQEQAYDYLKELILSGEIIENEIYSETKLASEIGISRT
                     PIRDALQRLSQDGFIDIIPSKGFRIHQITANEIIEIFQIRSAIEGFCTFLITSQYKEA
                     RAIETISKLKHLLDKQKDILLGDKNLNSFAEYDTLFHTTIVSYAQNTEFDKMFNNYMY
                     RIKKLALDSLSHEGRLETTLKEHTDIFNNIANGCIEDIYKTTLIHMETPKYINLEDFC
                     NNKLF"
     gene            13176..14081
                     /gene="garR"
                     /locus_tag="L3M60_002316"
     CDS             13176..14081
                     /gene="garR"
                     /locus_tag="L3M60_002316"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBN6226974.1"
                     /translation="MEVYKMKLGFIGLGIMGKPMAKNLLKDGCNLLVYDIDKSAVDEL
                     ISCGAKYASVLEMGRECDIVFTILPNGTIVQDVLFGADGLAKTLKEGSIVVDMSSVTP
                     TESVLCANKLKERGLEFIDSPVSGGEPKAIDGTLAFMAGGKEEIYKKVEPFFNIMGSS
                     SLLIGDNGSGSVTKLTNQVIVNLTIAAVSEAFVLAAKAGADPEKVYKAIRGGLAGSTI
                     LDAKIPMIMNRDFKPGGKISINLKDIKNVMQTAHNLDVPLPMTSQLLEIMQTLKVHGH
                     LEDDHSGIAQYFEKLAGVEIKKHNN"
     gene            14217..15599
                     /locus_tag="L3M60_002317"
     CDS             14217..15599
                     /locus_tag="L3M60_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBN6226975.1"
                     /translation="MITGYGLLVAFVISIGILLVSIIKFKVNPFLALLITSIITGFMV
                     RMPINEISTTISTGFGNTLGSIGIVIGLGIIFGNILSESRATESIAKGLLAKTGEKNS
                     ALAVTTAGFLISIPVFMDAAFVIMMPIIKYVSRVTKKSLMVFVCALGVGTIVGHALVI
                     PTPGPLAVAANVNANVGSFILYSIIVAFPAALIGGWIYGKRFEKYPAYAIDENDREKN
                     LEQGKDSIKIEDDDSSKVPGFRISMFSLLFPILLILISNVFSMFLEKGSTMSGVLAFI
                     GDKNIAILLGILVAIGFLKKYINKPMGDVVIEAADSAGLILLITGSGGAFGSVINASG
                     IGNFLVDTMSGLSISVVVLGFLLSALLRISQGSATVALVTTSSILGPTILATGMSPVL
                     VGLAICAGGVGFSLPNDSGFWVLSRFSGLSVKDTLNSWTIGGTIAGVVAFIMVLLLSV
                     INGIIPLPGL"
     gene            15671..>16097
                     /locus_tag="L3M60_002318"
     CDS             15671..>16097
                     /locus_tag="L3M60_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HBN6226976.1"
                     /translation="MREVKPISIDILNTFKQVDEDRLNKLLADELKHLDRKIVVLDDD
                     PTGVQTVHDISVYTDWDKDSMEQGFNEKNSMFFILTNSRGFTVAQTTKAHKEISKNIV
                     DVSKKVNKDFIIISRSDSTMRGHYPVETNLLKSEVERLSE"
BASE COUNT         6014 a         1913 c         2579 g         5591 t
ORIGIN      
        1 tataattata gaggggaaat ttaagatgga ttttaaaaat ttgcagtatg aaagtttgtc
       61 atatattatt tgtaatttac aaaaaaactt taaactttat tgtgaaaaat gtttaaaacc
      121 atataaactt actaatggtc tatattttta tttaatctat attaataaaa atagaaattg
      181 ttctttgaat gatgtttcta cagaatttga agttgataag gctcatacaa caagaactat
      241 atctagatta gaacaagatg gatacataga aaaaatccaa aaccctaatg atagcagagc
      301 ttttcagttg agagtaacag ataagggaga ggaagtatta ggagatataa aaaatatatt
      361 ttcaaaatgg gataaccgga taaaaaaaga gttctcagat gaagaatata aagaattagt
      421 gaagaaatta catgtggtaa aggatataaa aacagctatg gaggaagaat aggatgtatt
      481 gtaatttgtt aaagccaatt aaaattggaa atttggaatt aaaaaataga gtatcatttg
      541 cacctacaag catgggactt aaattagaag aaaaaattaa aaaattttgt gatattgcta
      601 agtcaggagt agctttaatt actttaggag atgtatctgt tagacctagc tttcataata
      661 tggctatgag cttgacagat gaagatggag ttatgaaata caaaaaaatc gtagacgaaa
      721 tacacaattc tggtgcaaag gtatcagcac aaatattttg ttcagattat gatgttaatt
      781 taattaaaga tactatgaag atgggaatta cttcacatgt tgagataaaa aaaataatga
      841 atgatggagt aaaagattac ataacaaata tgcccaaaga agaaattaaa aatattataa
      901 atttatttaa agtaacagca ttaaatgcaa aaaaagcagg atttgacatg attcaaattc
      961 atggagatag gcttatagga agtttttcat caagtatatt taataataga aatgatgaat
     1021 atggtggaac atgtgagaat agaagcagat ttgcaagtga aataattagt agtatccgaa
     1081 atgaagttaa ggatattcca atagactata aatttgcaat aagacaagaa aatcctcatt
     1141 atgggaatgc aggagtttta ttttcggaag ttgagtattt tgttaaaaaa tttgaatccc
     1201 taggtgtaaa cagttttcat gtaacacttg caaatcattc aaaattggaa gatactatac
     1261 caatcaataa ccatccttac tttaaagatg aaggttgttt tttatattta gctgatgaag
     1321 taaagaaaca cacaaatctt ccagtatgtg gggttggaaa attgtctagc ccagatttta
     1381 tagaaagtat tatttcaaat aatagagtag atatgatttc tatgtcaaga caattactag
     1441 cagatagtaa ttggttacag aaagtaaaag atggaagagt agatgagatt aaaaaatgct
     1501 gttattgtaa taaaaagtgt gcagatgcct tgcagacaca ttctcaattt ggatgtatct
     1561 tagattaaat tgtgtttgta gaaataaaaa atctgttaat atttttcttg taagttagat
     1621 aattatttag ataaatagga ggatacatca tgaaaagttt aatcttatat tcaagtttaa
     1681 ctggaaatac taaaaaaatt gcctgttcta tatatgaggc aattcaaggg aaaaaagaaa
     1741 ttaaaaatat aagtgaatta gttgatgata gtattgacta tgaaaattat gatatagtat
     1801 tcttaggcta ttgggtagat aagggaattt gtgataaaaa ttcaaaacaa gtacttgaaa
     1861 atattcacaa taaaaagata gcactatttg gaacaatggg agcaagtaca aaaggaagtt
     1921 atggagctag tataatagaa aagatagaat caattattcc aaaagataat gaaatattag
     1981 gttcattcat atgtcaaggt aagatagcag aaggattgaa agtaaaatat aaagaaatgt
     2041 taaaactttc tcctgataac gaacatattc gccaacaatt aaataaccat gaggaatctc
     2101 aaagtcatcc agatgaacaa gaaatatatg aagctaatgt atttgcaaaa aatatgatga
     2161 ttaaagctag tactatgtaa cagttaaata aagagtttat ataggatata gaatcaataa
     2221 tccaataaaa agtatagcac atgatttgct atgctttttt tatgaaatta aaaaaataat
     2281 aaaaatactt gactgtaaac cttgtttata cattatttta tagaaaataa gatagtttta
     2341 aatatagaaa aagagatatt attgaaagga gatattattg tatgaaaatt tataaaaggt
     2401 aattagtttg attttaagac ataataaaca tacaatttta tagagtgaaa gtatttaaga
     2461 ctatattata gctataaatt aatagaatag agaggtagtg tttaaaatga caattaaaga
     2521 agttgaagaa aaaacaggat tgacacgctc taatattcgt ttttatgaga acgaaaattt
     2581 aattaaaccc cttcgtaatg agcgtaatgg ctatagagaa tattcagatg aaaatataga
     2641 ggacatcaaa aaaattgcat atttacgtac attaggaatt actattgatg atatccgtaa
     2701 tattatatta cagaaagtat ctttagttga agttgtagag aaacaggaga aagttctcga
     2761 aactaaaata tcagatttag aaaatgcaaa aactatgtgt aataaaatgt tagagtctga
     2821 tgagctaagt tatttgaatt tgaaagtaga gcaatacata actgagttgc cagaatactg
     2881 gaataaaaac aaacctgttt ttaaacttga ctctgtaagt ttcttatata tatggggaag
     2941 gaaaattacc tggggaatta ttactattgc ttgtttgttg attgcaataa tagtatttcc
     3001 aaagttgcca catcaaattc ctatccaatg gaataatggt gtagctaatt atgtggataa
     3061 aaaatttatt tatgcctatc ctgctatttg tgttgtattc agaatccttt tacgtccatt
     3121 tatatggaga tggttacaaa tacatgcttt ttatagtgat actattgcag attatttaac
     3181 aaattttatg tgttttgtag cgttgtctat agaaatattc tctatacttt ttatttacaa
     3241 tattgccaga catgttacaa taattatttt tatagatata tttgtattta ttgctttact
     3301 gataataggc gtgtataaat tatttaaaaa atataattaa cttttaaaaa taagtaataa
     3361 gtgatagtgt aaaaagtagc cactgcaaat tatggattgt agtggccatt ttattatttg
     3421 tatatgaaat tattttattt ttctaataag ctacgatata ttatggttta tttcactgat
     3481 aaaagattta ggtattttaa agtaaaaagt tgtatattct aataaaattt aaacattttg
     3541 attctttaaa attagagaaa atacattgct tttattaaaa atttaaatat taagaattta
     3601 atttctgcat cttatttaga aaagataaga aatcaatttt atatattttt tttggtcttc
     3661 ctctttgatt atcgagtttt gtatttacaa tagttgcaat tcctgcttta tataagatac
     3721 tcaatattct attagcactt ctaagagtta tgttcatata gtctgctaat atttctgctg
     3781 acacattatt tgaatttcta tcattaatca tacacatgat tttagacaca tttgtattag
     3841 atagaggtat aaaagaagca actttattgg ctattgagct atcttcaata gtattagaat
     3901 ttttatttga atacaaaggt cctattgtat cattagtatc atttactata aaagtacagt
     3961 ttccatttag agcagcagat ttaccattag cttgactagc attttgttct gcatgaatat
     4021 ttgtatttcc aataccccaa cctatattta agctatattt aatttctttt aaatcttctg
     4081 aaataaaaaa atctgtatag ttattggtca tattgtttaa catttcctta gtagtatgta
     4141 tctctatctt catatcattt tttttgacct ggactccata aaatccatgt cttgatagag
     4201 aatttaagat agtgttatat atttggtttt gtatctcatc atttttaaat atcaaatctt
     4261 catttatgta ctctgaacta tttactgtaa ctatacaagt tgcggattta tttttgtcta
     4321 agttttgtaa tgttatttca ttcaccagag atttaaatgt attcaagatt gattctgtag
     4381 atggaaccat gtaaatacaa tcatatccaa gcttattaag ttcacaacta atgatgtaat
     4441 ttctagtaaa agaaaggtta atcttattat ctcgcaataa gtctaaatgt atttccaata
     4501 atttatcatt taatttttta aagtcataca caggttgagg gatgttaaaa tctttgattg
     4561 tatatggaca tttattagaa tctattaatg aatatatatc ataataattg ttgtcttctt
     4621 ttaaaaaatc cataaatgat tttgtaaagt caatatcttt ttgactattt aatagtttga
     4681 ataagtattt ataaaaatct ctttcatcaa gctttaaaaa gtttaatggt acttttatgt
     4741 cttttatatt tattataagt tcactatatg gaactatacc actacataca aatccattta
     4801 tatgaaataa agtatatttt cataatttca acaatgaaag agaggcttat ctctattttg
     4861 agatagcctc ttcttttaaa tttatatttc tagaatttat gtttttattt atacaacatt
     4921 gacattagtt attaacaata attttgagaa aaatatactt tcgctaaaac caaaataaat
     4981 gtatttttaa agtatgattt gctttgaatt ttcacgaata tctacttcta tgactctttc
     5041 tattgtatct cctctaaaac ctaataaatt actcgtcaag tttgcagtgg aacaagaatg
     5101 attaggtata attcttattc tatcaccaat ttttatatca gtttcaccat ttactttaat
     5161 ttttccaact tcttctgaaa gtgaatcaat agtaagttct ggatgtccac ataccctacc
     5221 ataaccaaca atactactgt ttccatgtgc acctttatca agtccaaggc acttacttcc
     5281 acaatcaatt aaatagtatc cactgctatt ttttgagatt acagtgcaca caacagtaag
     5341 agcacacata tcttcagtag cacaacctaa acctatctgt atagcatcat agtatacata
     5401 gtttccaggt cttacagcag ttatagtttt agaaacagct tcagcttcaa ctgttggagt
     5461 actaccagaa gcgacaattt ctatattaaa tccattatca gttagagctt tgtatgcttt
     5521 ctccatagaa gaatcttctt ggacacaaac atctggaaca ccttctggag agctacaacc
     5581 atacacttga cctggatgtg agcttatacc aacaatcttg atgttttcta gcttagaaat
     5641 ttcagtagca agttcaacca ctttttctgg ttcaacccca aaacggtgta agccacaatc
     5701 tacaataata agtagtttac actctagttt tgatttagaa aaagcttcac tataagcctt
     5761 agcacattct atattatctg tagcacaaat taaatttatt tttgatgcca tagtaacaat
     5821 tctatttata tttacctctc ccatgtaagg ataagccaac ataatatctt caaaacctgc
     5881 ttttatcaaa gcttcacctt catcaagtgt tccaactaga aaaccactag cacctgcatt
     5941 tttttgaagt ctcgctatgt aagtactttt atgtgtttta agcataggcc aaagttgttt
     6001 attattattt gtacaaattt gttgaatttt atctatattc ttttctaact ggtctaaatc
     6061 tagcaaaaaa tttggagtag aaacttctga aattttcatg ttatttacca cctttcaaca
     6121 aaagtaaatt aactataagt attataatta ttatattagt aatatcggct ttataatatt
     6181 aatagcttat aattatatta taaaactagc atgaacagag agcaatgttt atttgtaata
     6241 attagaattt tcaataaatt attggagttt taaattattt atataacata tttttaattt
     6301 tatgcaaagt ttatttaaat tagtttgagg atttattaat agggtatcat tgaatgagta
     6361 aaaaagtgat gtttgcatag aataattaca ctataaatgt tggaggtagt aaacatgcga
     6421 ggggtagtaa acatgcgaaa agtaatactt tatattgcca caagtttaga tggttatata
     6481 gctgaagaag atggcagtgt aagttttttg gacgagatat cttcagtctt taagaatgaa
     6541 caggaagatg attataatac tttttacgat acaattgata ctatcattat gggaaattct
     6601 acatatgtac agattgcaaa tgaattaagt ccaaatgaat ggtactataa ggggaaggag
     6661 tgctatgtat actcaaatat aattaatgag ataactgaaa atgtaaagta tacgaactta
     6721 gaacctaaaa aattaattga acaaattgga agagagaaag ataaaaaaga tatttggata
     6781 gttgggggag gcgatatagt aaaattattt atgaaagata acttaattga tgattattat
     6841 atatatatat tgccaataat attggggaga ggaatttcac tatttaagtc tggtatagac
     6901 aagactaact tgaattttaa gaaagctagt aatattggag aacttgtaaa attggaatac
     6961 agtaaaaaac aaaaataaat tacatgccaa tttgttacaa tgttatgaaa tgaagcattt
     7021 aaaagacttt tggctatatg aattgacatt acttgtcaaa tatgttaaca tataagcgac
     7081 ttgtcggagg gtgtattaaa aagaatatgc tggagaagag agtgcgttaa ctcttttctc
     7141 ctttttttat aaaattaagc atttataaat ttagtattat tatatgatta cactagctca
     7201 aaaattaaga aaggagaaaa atatgaaatt aattttaagt tatttaaaga attataagtt
     7261 attaatagta ttaaacatac tggctatatt tagttttgca ttagtcgaat tggggattcc
     7321 aaccataata gctaaaataa ttgacaatgg tattgcaaat cagaatatag cttacataaa
     7381 acaaatgggg atagtaatag ttattatttc tattataggg gttgtaggta gtgtattgtt
     7441 gggttattgt tctgctaagg tatcaacaag tgtaacaaga gatataagaa atgatatatt
     7501 tgagaaatca caagaatttt cacatactga atataataaa tttggaattt cttcaatgat
     7561 tacaagaaca acaaatgacg catttcaaat tcaacaattt gttaatatac tactaaggac
     7621 tgccttatta actccagtta tgttcatcat aagtataata atgacaatta gaacaagtgt
     7681 ggaactttct atagtattgg ctatatcagt accttttata attattggag tagcaataat
     7741 agcaaaagta tctcaggcaa tatcttcaaa gcaacaaaaa ggactagata aattaaacct
     7801 tatatcgaga gaaaatttaa ctggaataag agtaattaga gcatttggaa atgatgatta
     7861 tgaaactgag cgatttgaaa aaacaaatac atattatgct aatgtttcta agaaattatt
     7921 taagcttatg tcaattactc aaccagcatt ctttttacta ttaaacatag ctgttttagc
     7981 tgtattttgg atttcaagtg agaagataaa cattggagaa cttcaagttg gacagttggt
     8041 tgcatttttg gaatatcttt tccatgccat gttttcaatt atgctttttt caatggtatt
     8101 tattatgtat ccaagagcag aggtatctgc aaatagaata aaagaacttt taaatgaaga
     8161 accattaata aaaaatcctg aaaatggaat taaggagaat gaaaataagg gtatcataga
     8221 atttgataat gtaactttta cctatccaga tggagaagct tctgtactta aggatatttc
     8281 atttactgca aaaacaggtg aaacagttgc ttttatagga agtactggta gtgggaaaag
     8341 tactctaatt aacttgatac ctagatttta cgatgtaaca gaaggtagca ttaagataaa
     8401 tggagtagat ataagagaat atgatttaaa agctttaaga aaaaagatag gattcatacc
     8461 tcaaaaatca ctgcttttta ctggtagcat agcaaataat attagatttg ggaaacataa
     8521 ggcaggagag tcagaattag aatattccgc aaaagttgca caagcatatg agtttatatc
     8581 aaaaaaacct agaaagtttg atgaattgat tagtgaaggt ggagcaaatg tatcaggagg
     8641 tcaaaaacaa agactttcta ttgcaagggc tattataaga agacctgaaa tatatatttt
     8701 tgatgatagt ttttctgcac ttgactttaa aactgatgca atacttcgtg ctaaactgaa
     8761 gaaggaaaca aaagatgcaa ttgtattgat tgtcgctcag agaattagtt ctattattga
     8821 tgctgataag attatagtac tcaatgaagg acaagttgtc ggaatgggaa ctcataaaga
     8881 attattaaaa aactgtgaaa tatactatga aattgcaaca tctcagttaa aaaaggagga
     8941 attagaataa tgaaaaagtc taaaagtttt aagaaaacaa ttggtagact tcttccattt
     9001 gtaaagaaat ataaattttc ctttattata gctattatat gtataatttc agcagctact
     9061 atgaatgcac ttgcacctaa aactgaggga ttgataataa cacaattgac taaagatgta
     9121 attagtatag caaaaggaat accaggagca tccgtaaatt ttgactatgt aacaaaaata
     9181 ttggtgattt tagcatgtat atatgttgcc aatgcaattt ttacgtatac ttcaagtttt
     9241 ctacttacga atgctattca aaacacaatg agagatttaa gaaatgaagt agaaaataaa
     9301 ataagaaggt tgcctattag ttattttgat agtaatagtt ttggggatgt attaagtcgt
     9361 atatcaaatg atgtagatac aatatcaaat gctttgcaac agagttttat gcaaattgta
     9421 aattcaattt tagtcataat tttggcactt tcaatgatgt ttacaattaa tatatatatg
     9481 gctcttatag ctctttttat aattccaatt agttattttg tatctaaatt tgtagtaaaa
     9541 aaatctcaaa atagattttc attacaacaa aatgctcttg gaaaattaaa tggaaaagta
     9601 caagaaatgt atactggatt taatgaaata aaactatatg gtaaagaaga agactctata
     9661 aaagagttta aaaaggtaaa tcaagaactt tgtgaaaatg gatttaaggc acaatttata
     9721 tcaagtatga tgaatcctat ggtttcatta gtaacttact ttggaattgc agcagtggca
     9781 gttgtaggtt ctatatatgc agtaagtgga gggataacag taggaaattt acaagcattt
     9841 gtgcgttata tatggcagat aaatcaacca ctttcacaaa tgactcaatt atccacagta
     9901 atacaatctt cttttgcagc tatagagcgt gtatttgaaa ttttagacga agaagaggaa
     9961 atacctgatg ttgaaaatcc agttaagata gaaaatgtaa aaggaaatgt tacatttgag
    10021 catgttaatt ttggatatgg agaaaattca actttaatag aagatttaaa tgcagaagta
    10081 aaaagtggtc agatggtagc tattgttgga cctactggag caggtaaaac aacattaata
    10141 aacttattga tgaggtttta tgatgtaaag aaaggtgcta ttaaaattga tggagtagac
    10201 ataagagata tgaaacgtaa agatttacgc tcaatgtttg gtatggtact tcaagataca
    10261 tggttattta atggaaccat ttttgaaaat atagaatacg gtagatttgg agctactaaa
    10321 gaagagatta tacaagcagc aaaagtagca aatgttcatc actttataac cactcttcca
    10381 gatggataca atatgttttt aaatgaagaa gcatctaata tatctttagg agaaaaacag
    10441 cttttaacca tagctagagc ttttatatca gacccttcaa tattgatact tgatgaagct
    10501 actagttctg ttgatactag gttggaactt atgcttcaaa aagcaatgag aaatcttatg
    10561 aatggtagaa ctagttttgt tatagcacat agactttcta caattcgtaa tgctgacttg
    10621 attttagtta tgaataatgg aagcattata gaacagggca atcatgatga attgatggaa
    10681 aaaggtggat tttatgaaaa gctttataat agccaatttg cagacacaga atcagaatag
    10741 ataacataaa cttttgtatg aaattttgta tcaaattcaa atattaatca tataattaaa
    10801 aagagtatca gtaagttgtt ttttatagat ttactgatac tctttgattt taagaccaaa
    10861 ggtacttatg gttggtactt ataattcgta actagtcttg ttacatctga taaccagaat
    10921 tacagtactt ttctttttta cctttttgtt ctctaacata taacaattcg cttaacttat
    10981 caatgtaata accatcatca cttatacgaa ttaaactagt cagataatat ccatctttgc
    11041 ttctaggaat ctttttattt attgctaaat caatagctgt ctttgctgct tcaaaatgct
    11101 ttaaatgtgt atgaccttct tcaaaactct ttctagtatt ataaactaca taaccttttt
    11161 ttacttgaaa aactataaat tctttttttt catatacttt attaaacatt caatcagccc
    11221 ttcttgagat agttttatag cataacatta gaagcttcta aaaaatttta caaagaacat
    11281 aaatatttta taaattataa atctaaaact ataatttata aagataactt tctgattatt
    11341 tatatgataa ttcatataga ttaaaagtaa attatcttta ggttaaacaa aagtaaaata
    11401 aaagactagt tataatattt atatgaaaaa gtacaaaata tagtatatga atatagaaaa
    11461 tttaaattat ttatttacag tttttatttc cataagtata tttaccttca gataccttgt
    11521 catcatgaaa atatattctt tcacaacttt tatctcccca taaaatgttt tttgttattt
    11581 tatgggaggt atctcctgta aatacttcag ctacagtagt atctgtaaca tcacaataaa
    11641 gagaagcaaa ctttttaaac caaggatttt cctcaaatct taaaagccag gaagctccat
    11701 aagggcaatg caactcttca aattcaggaa cccatcctag aaatggaaca tcagttgtgc
    11761 taataatatt gtctgcaatt aattcatctc cattttcatg agctctttct ctcatagcaa
    11821 tagctctttc ttcagacctt tcaaataaaa ctttttttaa tatttcgcta gttttatctt
    11881 caccaaactc ttcaaataac actttggaaa aataaaaata taagtcacca aaatgcttgc
    11941 atgccatacg aacttcttca atagcataat ctttgtcgat agtttcatta gttacaacat
    12001 cttcgttcaa aaaaatcact ccttagaata gtattagtta caatataatt atagaatatt
    12061 tttaataaat taggaaatta atttaagata tatatttttt tagtaaataa aagtaaaatg
    12121 ctaaaataac ttattgttac agaaatcttc taaattaatg tatttaggag tttccatatg
    12181 aattagagta gttttgtaaa tatcctcaat acatccattt gcaatattat taaaaatatc
    12241 tgtgtgttct tttaatgttg tttcaagtct tccctcatgt gacaatgaat cgagagctaa
    12301 tttttttatt ctgtacatat aattgttaaa cattttgtca aattcagtat tttgagcata
    12361 tgaaactata gttgtgtgaa ataatgtatc gtattctgca aatgagttta aatttttatc
    12421 acccagtaaa atatcttttt gtttatctaa aaggtgtttt aatttactta tagtttctat
    12481 tgccctagct tctttgtatt gacttgtaat taaaaaagtg caaaaccctt ctattgcaga
    12541 gcgtatttgg aatatctcta taatttcatt tgctgtaatt tgatggattc taaatccttt
    12601 gcttgggatt atatctataa atccatcttg actaagtctt tgaagagcat cccttatagg
    12661 tgttctagag atgcctattt cactagcaag ttttgtttca gaatatattt cattttctat
    12721 tatttctcca gatagtatca attcttttaa gtagtcatat gcttgttctt gtaatctttt
    12781 aaatgtagcc atacttaata atacccccct aaacaatata aaaatacaaa aacttatata
    12841 attttaagca tacaacatag taaaaataag gtcaatcttt tgaaatttaa attgcatata
    12901 agattaaaaa atataaaaaa ttgttgaagt gtgatatatg attaacacta aagtcagatt
    12961 aattaaaatt agttcaaata caaaactgta aaagtagtat atgatattta aaaaatagtg
    13021 tatattaaaa cttatataga ataactaaaa ataataaata actttaaaaa ttactattta
    13081 ttgttaaaat aaatactttt atattttata ttgaccggta tactggtatg tagtatactc
    13141 taaaacataa aaaacgtttt caaaaatgtg aagatatgga ggtctataaa atgaaattag
    13201 gatttattgg attaggaata atgggaaagc caatggcaaa aaatttattg aaagatggat
    13261 gtaatctttt ggtgtatgac attgataaat ctgctgtgga tgaacttata tcatgtggag
    13321 caaagtatgc aagtgtttta gaaatgggtc gagaatgtga tattgtattt acaatccttc
    13381 caaatggaac tattgtgcag gatgtattat ttggagcaga tggattggca aaaactttaa
    13441 aagaaggaag tatagtagtt gatatgagtt cagttactcc tacagaatcc gtattgtgtg
    13501 caaataaatt aaaagagagg ggcttagaat ttatagattc accagtaagt ggaggggagc
    13561 caaaagcgat tgatggtacg cttgctttta tggctggtgg taaggaagaa atttataaga
    13621 aagtagagcc attctttaat ataatgggaa gttcatctct tttaattgga gataatggta
    13681 gtggaagtgt tacaaaactt acaaatcaag taatagttaa tttgacgata gcggcagtct
    13741 ctgaggcatt tgtacttgcg gcaaaagctg gtgcagaccc agaaaaagta tataaggcaa
    13801 taagaggggg gctagctggt agtacaatat tggatgcaaa gataccaatg attatgaatc
    13861 gtgattttaa accaggtgga aaaatatcta ttaatttgaa ggatattaag aatgtaatgc
    13921 aaacagcaca taatttagat gtaccactac ctatgacaag tcaattatta gaaattatgc
    13981 agacattaaa agtacatgga catttggaag atgaccatag tggaatagca cagtattttg
    14041 aaaagttagc aggtgttgaa ataaaaaaac acaataatta atacttaaat atatcaatat
    14101 aactaaatac atactattag gattagatta tataaataca gcaaggttaa attatcatat
    14161 taaaagaaaa ataagattga taaataataa agtagaatga taaaggggta aagattatga
    14221 ttacaggata tggacttttg gtagcatttg ttatatctat aggaatttta cttgtatcta
    14281 ttattaaatt taaagtaaat ccatttttgg cattacttat aacatctata attacaggct
    14341 ttatggttag aatgcctata aatgaaatct caaccacaat atcgactgga tttggaaata
    14401 cattagggag tataggtatt gtaattggac ttggaataat attcggaaat attctttcag
    14461 aatcaagagc aacagaatct attgcaaagg ggttgttagc taagactgga gaaaaaaaca
    14521 gtgctttagc agtaactaca gcaggttttt taatttcaat tccagttttt atggatgcag
    14581 ctttcgttat aatgatgcct ataattaagt atgtttcgag agtaactaag aaatctctaa
    14641 tggtttttgt atgtgcatta ggagttggga ctatagtagg tcatgcactt gtaataccta
    14701 caccaggacc tttggcagtt gcagcaaatg taaatgcaaa tgtaggtagt tttatacttt
    14761 attctataat tgttgctttt ccagcagctc ttataggtgg atggatttat ggaaaacgtt
    14821 ttgaaaaata tccagcttat gctattgatg agaatgatag agaaaaaaat ttagaacagg
    14881 gaaaagatag cataaaaatt gaagatgatg attcttctaa agtaccaggt tttagaattt
    14941 ctatgttttc tttattgttc ccaatacttc ttatacttat atcaaatgta tttagtatgt
    15001 tcttagaaaa aggttctaca atgagtggag tgcttgcatt tataggagat aaaaacatag
    15061 caatacttct tggaatattg gtggcaatag gatttttaaa gaaatacata aataaaccta
    15121 tgggagatgt agttattgaa gctgctgatt cagcagggct aatattattg ataacaggtt
    15181 caggtggtgc gtttggcagt gttataaatg caagtggtat aggtaacttc ttagttgata
    15241 ctatgtctgg tcttagtata tcagttgtag tgcttggatt tttattaagt gccttgctta
    15301 gaatttctca aggttctgct actgtagcct tagtaacaac atcatcaatt ttaggaccta
    15361 caattttggc aactggaatg tcaccagttt tagtagggct tgctatatgt gctggaggag
    15421 ttggtttctc ccttccaaat gattctggat tctgggtact ttcaagattt agtggattat
    15481 cagttaaaga tactttaaac tcttggacaa ttggaggaac cattgctgga gttgtagcat
    15541 ttataatggt tttattgctt agtgttataa atggaattat accacttcca ggtctataaa
    15601 taacaaacta ttgtttatat tatataaaat tacatttaga ctatacaaat tacataaagg
    15661 ggcgttttaa atgagagaag taaaaccaat tagcatagat atacttaata cttttaaaca
    15721 agtagatgag gacagattaa ataaacttct tgcagatgag cttaagcatt tagatagaaa
    15781 aattgttgta ttagatgatg acccaacagg tgtacaaaca gttcatgata tttctgttta
    15841 tactgattgg gataaggata gtatggaaca aggttttaat gagaaaaata gtatgttttt
    15901 tatacttact aattcaaggg gatttactgt tgctcaaact acaaaagcgc ataaagaaat
    15961 ttcaaagaat attgtggatg tatcaaagaa agttaataag gattttataa tcataagtcg
    16021 tagtgattct actatgagag gacattatcc tgttgaaact aatttattaa aaagcgaagt
    16081 agagagatta tcagaga
//
LOCUS       DAFNNB010000089        15859 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0090, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000089 DAFNNB010000000
VERSION     DAFNNB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15859
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0090"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..2558)
                     /gene="carB"
                     /locus_tag="L3M60_002319"
     CDS             complement(<1..2558)
                     /gene="carB"
                     /locus_tag="L3M60_002319"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBN6226977.1"
                     /translation="MPKLDSIKKTLVLGSGPIIIGQAAEFDYSGTQACQSLKEEGIEV
                     VLINSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     YEKGILDKYGVKIIGTSVESIKKGEDRDIFREVMKEINQPVIVSDIVTDLQAGLDYAL
                     TIGYPVVVRPAYTLGGTGGGIAENEEELREILSHGLQLSPVGQVLIEKSIKGWKEIEY
                     EVIRDSKGNCIVVCNMENIDPVGVHTGDSIVVAPTQTLSNKECAMLKKASLDILNAVE
                     VQGGCNVQFALNPHSFEYAVIEINPRVSRSSALASKATGYPIAKVSSKIALGYTLDEI
                     ENAVTKKTYACFEPTIDYVVAKIPKWPFDKFKKANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHTPSEERSIEELKKRVVVPDDERLFDLAEMIRRGYKVEMIEQ
                     ITGIDKWFINKFKWIVEQEEKLKVMKIEDLTKDYLLELKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIKPAYKMVDTCAGEIDAISPYYYSTYEQYDEVVVSDKKKVIVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIINNNPETVSTDFDTSDKLYFEPLTEEEVLSI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFDDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWSLNEGIEVAKELGYPVLVRPSYVLGGQGMEITYNEEKLSQYLQDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGV"
     gene            complement(2611..3654)
                     /gene="carA"
                     /locus_tag="L3M60_002320"
     CDS             complement(2611..3654)
                     /gene="carA"
                     /locus_tag="L3M60_002320"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBN6226978.1"
                     /translation="MKGKLILEDGTVFEGKAFGYLKETIGEVVFNTSMAGYGELLTDA
                     TYYGQIVTMTYPLIGNYGINLEDVESNKVQVKGLIVREKSDNPNNFRCEMDIDTYLKQ
                     NKVIGLEGIDTRALTKIIRNNGTKRGIITLENVSLQDVQSKLEEHCNKDSVKNVTRKE
                     TENIKGTGAKVTVIDFGVKNSVLESLKACNCDITIVPAMTTAEEILNINPDLILLSNG
                     PGNPEDINDIVENIKKLIGKKPLTGIGLGHQILALALGGKTSKLVFGHRGGNHPVKCL
                     ETGKVFITSQNHGYNVSEMPENTEVTHINLNDNTIEGMRHKELPIYSVQYIPEYTTKE
                     DLDCIFNKFLALI"
     gene            complement(3680..4396)
                     /gene="pyrF"
                     /locus_tag="L3M60_002321"
     CDS             complement(3680..4396)
                     /gene="pyrF"
                     /locus_tag="L3M60_002321"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBN6226979.1"
                     /translation="MINGKEKLIVAIDTDEFDKAKELIDKLEDSVDIFKVGLEQYVAT
                     KGKTIDYLKEKGKKIFLDLKFHDIPNTMKSAVRAAVRDNVWLMTIHVSDMEGMRQCAL
                     VAKEEAERLNIEKPLVVGVTVLTSLSNQDLQDIGCNMTTEELAIKRAKLAKESGIDGV
                     VCSAQEVDKIVEACGTDFITVCPGIRPKSAEVGDQKRVVTPSDAIKRGAHYLVVGRPI
                     TKAKDPRESAINIVREIENA"
     gene            complement(4772..5407)
                     /locus_tag="L3M60_002322"
     CDS             complement(4772..5407)
                     /locus_tag="L3M60_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1847 domain-containing protein"
                     /protein_id="HBN6226980.1"
                     /translation="MYKCGKCNLRGCSNGNMDKLMKICPTREIELQQKAKQLYEEEEN
                     KKIAYNAGLVEAEGYCQYTRLKEIIEFAKKCGYKKIGIAFCIGLKREMQTVQKVFEHH
                     GFEVFSVICKNGAIPKSTIGLEASQTISKCSDEVMCNPIGQALFLNEARVDLNVILGL
                     CVGHDTLAMKYMEAPITTLVVKDRVTGHNPVAAIYNAESYFEKKLFDKDEE"
     gene            complement(5431..6345)
                     /locus_tag="L3M60_002323"
     CDS             complement(5431..6345)
                     /locus_tag="L3M60_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226981.1"
                     /translation="MELYEAIFYRKSVRNFSSKKIKKPLMEEIKRSCKNLEYLNEDLN
                     IKAHVVDRGHIIHFLMGKECKVKAPHYIVVTSDKGEDYLQNIGFATEGVVLRLTTLGI
                     ATCWLEGNLKREDILEFVDIGNKSFEEDEEDDLDYLVGIKTEEEDENKIENPYIIIAF
                     GYPAKNEELFRTRYARPDRKPVKDMINKINRNRKWKDILELTRRAPSVKNSQPWYFHK
                     DERGLHLFEKKPKKHCEDMNKVSLGVALRHFDIACIKNKIDVSYEKLPIRNKIGKSYF
                     ITVVEHVKPEEEIQEENVSLEKEELQDE"
     gene            6947..8176
                     /locus_tag="L3M60_002324"
     CDS             6947..8176
                     /locus_tag="L3M60_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBN6226982.1"
                     /translation="MNLNRNLEKYAELAIKVGVNIQPGQILLIKSPIECADFARNALK
                     EAYKCGAKNVYIEWSDEESTLIKYLYAPDEAFHEFPKWTAEQYVDIAKEGGAFLSVYA
                     QNPDLLKDVDPERVANFQKASGKALKEWRSYTLSDKCKWSIVSVPTKDWAKKVFPNLS
                     EDKAIEKLWDAIFKCSRVDGQDPIKAWEEHNKNLKSKMDFLNKNNFKTLKYKSSKTDL
                     TLDLPEGHVWLSGASKDPNGVSFNPNIPTEEIFGMPHKFKVNGTVYSTKPLVYGGNII
                     DNFFLTFKDGKIIDFSAEKGLDTLEKLIETDEGSHYLGEVALVPYNSPISNTDIIFYN
                     TLYDENASCHFAIGSAYKTCLEGGNNLKDEELDKYGVNDSLTHVDFMIGSADMDIIGE
                     TYDGKQIQIFKNGDWAF"
     gene            complement(8381..10762)
                     /locus_tag="L3M60_002325"
     CDS             complement(8381..10762)
                     /locus_tag="L3M60_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HBN6226983.1"
                     /translation="MEIRQYLKNNILIFDGAMGTMLQQKGLKLGENPEVFGIQNPEKL
                     IEIHTAYLEAGSNVILTNTFGCNELKLDSEYTVEEVIDNAVLVARKAIENVDNTKPRY
                     VALDIGPIGEMLEPMGTLSFDKAYEIFKRQVLQGVKSGVDVIVIETMMDLYEAKVAVL
                     AAKENSDLPIFCTMTFDEGGRSFTGCMPECMVATIEGLGVDAIGVNCSLGPKQLLPIV
                     EKIASRATVPVMVQANAGLPNIVDGEAIYDVDAKEFFEGVKKFVEVGATIIGGCCGTN
                     PSFIKEISENINSVTKGCIEKIDKCVVCSPSKFVEVESPTVVGERLNPTGRKALQEAL
                     KNENVDYAINLGLEQVNAGAQILGVNVGLPEIDEKKLMPKLIREIQAVVDTPLQVDSS
                     NVEALEQGLRYYNGRTIVNSVNGKEESLKSILPIVKKYGSCVVGLTLDEKGIPKKAEE
                     RFEIAKRIVDRAVSYGIKPKDIFIDCLSLTVSAQQEEAIETIKAIAMVKTLGVKTILG
                     VSNISFGLPNRKALNASFLTLALGAGLDLAIINPNECSMMEAINSFKILNNTDKGCIN
                     YINQYSNINNSKKLDSSTKIDKDLPLDILVERGLKDEAKNVTLNLLKEKDENYILDKI
                     LIPALDKVGKRYDSGDIFLPQLIQSAETVKVSLNTIKETLLSKSSNNVSKGKIIVATV
                     KGDIHDIGKNIVKIMLENYGYEVIDLGKDVPIEEVVEVAKKRNIKLVGLSALMTTTVQ
                     SMKDTIQALRDNEINAKVFVGGAVLTEEYAEEMGADYYSKDAKSAVEIAKLNF"
     gene            complement(10734..11459)
                     /locus_tag="L3M60_002326"
     CDS             complement(10734..11459)
                     /locus_tag="L3M60_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Vitamin B12 dependent methionine synthase,
                     activation domain protein"
                     /protein_id="HBN6226984.1"
                     /translation="MENNLIGREDISINQSEVLRYLQYRGQEIDKDLKDTIDECVAIT
                     KNKINPRYLSRIYPIRINKDNIVELEGTNLTLESKDVYELLKECNECVIMAATIGIDI
                     EKEIRRHSYSDLTKGLIIDSCGTTAIEEVCDIVQDNVEKVFLKQNKFITMRYSPGYGD
                     LPIEKNIDILNVLNAQKQIGLTITSNGIMIPRKSVVAIIGISDTRINKSKKEKSCENC
                     KNYRNCTYRKGANSCGNKTISEK"
     gene            complement(11449..11904)
                     /locus_tag="L3M60_002327"
     CDS             complement(11449..11904)
                     /locus_tag="L3M60_002327"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBN6226985.1"
                     /translation="MEKVESFKLDHTKVKAPFVRKCSVLDGVKGDKVTKFDLRFLQPN
                     VESFGTAAMHGLEHLLATYLRDTLDGVIDLSPMGCRTGFYLILWGDVDAKTVKIGLEE
                     ALKKVLESDKMPAATAIECGNYRDLSLFGAKEYAKDVLDKGFSLNIYGE"
     gene            complement(12186..13367)
                     /locus_tag="L3M60_002328"
     CDS             complement(12186..13367)
                     /locus_tag="L3M60_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBN6226986.1"
                     /translation="MKNKSLLNHMFRPILDLIISCALTSMLIVTSMYIIKSLYFNGAI
                     SVINQLVYQLRDFRSLIGESLFYPFLFTITSLAIYSIISYKRNKQIVDFIRKTNRKNQ
                     KLEIIKSAVNLMDEGNLEKSIDIEYDLDILKDNEKDDIDELAYKINNIVHQLRNITIE
                     ERQAQQTKTDLITNVSHDLRTPLTSIMGYLGVIEEGKYKDEVQMMYYVDIAYEKSKNL
                     NVLINDLFELTKMQNNTIKLNKIEINLVELLSQVISQFEIYFKQEFMVSRINFNEEKL
                     IVKADPNKLVRAFENLITNAVKYGKDGLYVDIVTEKKGDIAVVKVINYGEPIPVLDLP
                     NIFDRFYRVEKSRNRNVGGSGLGLAITKNIVNLHGGEITVSSDKYQTVFEVQLPIIQS
                     A"
     gene            complement(13357..14049)
                     /locus_tag="L3M60_002329"
     CDS             complement(13357..14049)
                     /locus_tag="L3M60_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6226987.1"
                     /translation="MKNTILVVDDEKEIRDLIEIYLLNSGYNVIKAEDGKEALDILKK
                     ENIHLMVLDIMMPKMDGIEVCRKVRESLNIPILMLSAKSEDMDKIQGIMTGADDYLTK
                     PFNPLELTVRVKSLIRRAYYFSGANNEDKDVIEVGAVIIDKEKHSVTVAGNDIILTSR
                     EFDILYLLANNKGRVYSTEEIFEKVWKEKYYQSNNTVMVHMSRIRDKIEKYTDGEKII
                     HTVWGVGYKIEK"
     gene            complement(14183..14899)
                     /locus_tag="L3M60_002330"
     CDS             complement(14183..14899)
                     /locus_tag="L3M60_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HBN6226988.1"
                     /translation="MKKFKLIVAIILVVFISVFISKDRLLKKDKEPTVIAQHYSKQDV
                     EANSQNKNHSNSISNILLVNKTNGISKNYTPENITKVNIPFVEEATEEEKQMAGEPAK
                     AVEDLVKQANSEGIQFLGSSAYRSYDTQLDTYTRRVKSQGREKADAYVAKPGYSEHQT
                     GLCIDLTNPERWFVGSTKEAKWLAENAHKFGFIIRYPEGKEDITGTAYEPWHIRYVGK
                     DAAEEIYSKGLTLEEYLQNK"
     gene            15025..15759
                     /locus_tag="L3M60_002331"
     CDS             15025..15759
                     /locus_tag="L3M60_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis protein TtcA"
                     /protein_id="HBN6226989.1"
                     /translation="MSIQKLLSKARKAIQDFDMIQENDKIAVGLSGGKDSLTLLHILK
                     SYQRFSPQNFELIAITLNTGGVDNSPLDKLCKEINVPFYEFQTDIKEIVFDIRKEKNP
                     CALCANLRRGALNDNAKKLGCNKVALGHHKDDAIETFLMSMFYEGRVNCFSPKTYLDR
                     QDLTVIRPMIYIEEYMTKKISKDSNYPIITNPCPANGHTRREYIKNLIANLNKEMPDF
                     KRNVFGALNNSEKLFIWDKEKIKNFK"
BASE COUNT         4857 a         2859 c         1756 g         6387 t
ORIGIN      
        1 actccactta ctttgcttat atatggaact gttctactag ctctaggatt aacttcaatt
       61 atatataatt cattttgaaa ttctataaac tgtatattaa ccataccaag cacattaagg
      121 tctaaagcta tttttttagt atattctact attttttctt ttatttcatc agatatattt
      181 tgacttggat acattgttgt actatctcca gagtgaactc cagctctttc taaatgctcc
      241 attataccag gtattaatat atcttcctta tcacatatag cgtctacttc tatttctcta
      301 ccagttagat atttatctat aagtactgga ttcttatggt ctctatcaaa tgcatcttgt
      361 aggtattgag ataatttttc ttcgttataa gttatttcca taccttgacc accaagtaca
      421 taagatggtc taactaatac tggataacct aattccttag caacttcaat accttcgtta
      481 agtgaccata caccttttcc ttttggtcta tttatatcta gtctctctag taaatcatca
      541 aacttctctc tatcctctgc tgcatctata tcatcaaaat ctgttcccag tataggtata
      601 tttttttcat gtaagaactt agccaatttt atagctgtct gtccaccaaa ctgtagtatt
      661 acaccttctg gtttttcttt ttctattata cttaaaactt cttcttctgt taatggttca
      721 aaatataact tatctgatgt atcaaaatct gtacttacag tttcagggtt attgtttatg
      781 attatggttt ctatgtccat ttttcttaaa gattttacac aatgaactga acaataatca
      841 aattctatac cttgacctat tcttattgga ccagagccta gtactatcac ctttttctta
      901 tcactaacaa ctacctcatc atattgctca taagttgaat aatagtaagg tgatatagcg
      961 tctatctcac ctgcacatgt atcaaccatt ttatatgctg gcttgatatt gtatagactt
     1021 ctcaattcat ataatttttc tgggcttatc ttcattaagt cagatatacc tttatccgag
     1081 aatccctttt tctttaattc aagaagatag tctttagtta aatcttcaat tttcataact
     1141 tttaattttt cttcttgctc tactatccat ttgaatttat ttataaacca tttatctata
     1201 cctgtaattt gttctatcat ttctacttta tagcctcttc ttatcatttc agctaagtca
     1261 aataatctct catcatcagg cactacaact cttttcttta attcctcaat acttctctcc
     1321 tcagatggtg tgtgaaccaa tgaatatttt cctgtttcaa gtgaccttat ccctttaaga
     1381 atagcagcct caaagttact tcctatactc atgatttcac cagttgccat catcttagtg
     1441 cctaattttc tatttgcttt tttaaattta tcaaaaggcc attttggtat ttttgctacc
     1501 acataatcta tagttggttc aaaacaagca taagtttttt tagttactgc attttctatc
     1561 tcatctaaag tataacctaa agctattttt gacgaaactt tagctattgg atatcctgta
     1621 gcttttgatg ctaaagctga tgatctactt actcttggat ttatctctat aactgcatat
     1681 tcaaaactat gtgggttaag tgcaaattga acgttacatc caccttgaac ttctactgca
     1741 tttaatatat ctaaagatgc ttttttaagc attgcacatt ctttgttact taaagtttga
     1801 gttggagcaa caactatact atctcctgta tgaactccga ctgggtctat gttttccata
     1861 ttacatacaa ctatacaatt tccctttgaa tctcttatta cctcatattc tatttccttc
     1921 cagcctttta tactcttttc tattaatact tgtccaacag ggcttaattg tagtccatgt
     1981 gaaagtattt ctcttaattc ttcctcattt tcagctatac ctccaccagt tccacctagt
     2041 gtgtatgctg gtcttacaac tactggataa ccaattgtaa gtgcatagtc aagacctgct
     2101 tgtaaatctg taactatatc actaactatt acaggctgat ttatttcttt cattacttct
     2161 ctaaatatat ctctatcttc accttttttt atagattcta ctgaagtacc tattattttt
     2221 actccatatt tgtctagtat acctttttca taaagttcaa ctgctaagtt taatcctgtc
     2281 tgaccaccca tacctgcaag taaactatct ggtctttctt tttctattat tttttctata
     2341 aattctattg ttaatggttc tatatatatt ttatctgcaa tctctttatc agtcattata
     2401 gtagctggat tactatttat taatacaact tcaattcctt cctctttcaa agattggcaa
     2461 gcttgtgttc ctgaatagtc aaactctgca gcttgaccta ttattattgg tcctgaccct
     2521 aatactaaag ttttctttat actatctaat ttaggcataa atttttctcc ttatctaaat
     2581 ggtttttact atctgaaaat aatactaaat ctatattaat gctaagaatt tattaaatat
     2641 acaatctaaa tcttcttttg ttgtgtattc tggaatgtac tgaacactat atattggtaa
     2701 ttccttatgt ctcattcctt ctatagtatt gtcatttaaa tttatgtgag ttacctctgt
     2761 attttctggc atttctgaaa cattatatcc atggttttga gaagttataa atacctttcc
     2821 agtttctaaa catttaactg ggtgattgcc tcctctatgt ccaaatacaa gttttgaagt
     2881 ctttcctcct aatgccagtg ctaaaatttg atgtcctaaa cctattcctg tcaatggctt
     2941 tttgcctatt aattttttta tattttcaac tatatcattt atatcctctg gatttcctgg
     3001 tccattagat aataaaatta agtcaggatt tatatttaaa atttcttctg ctgtagtcat
     3061 tgctggtact atagttatat cacagttaca agctttaaga gactctagta cactattttt
     3121 aacaccaaag tctataactg taaccttagc accagtacct tttatatttt cagtttcttt
     3181 tcttgtaaca ttttttactg aatctttgtt acaatgctct tctaacttag actgaacatc
     3241 ttgtaatgat acattttcta aagttataat tcctctctta gttccattgt ttctaataat
     3301 tttagttaat gctcttgtat ctattccttc aagtcctatt accttatttt gctttagata
     3361 tgtgtctata tccatttcac atctaaaatt atttgggtta tcacttttct ctcttacaat
     3421 aagtcctttt acttgcacct tatttgattc aacatcttct aaatttattc catagtttcc
     3481 tataagtgga taagtcatag taactatttg tccataataa gttgcatctg tcaatagttc
     3541 tccatatcca gccattgaag tattaaatac aacttctcca atagtttcct ttagatatcc
     3601 aaatgctttt ccttcaaata ctgttccatc ttctaaaatt aatttcccct tcatataagc
     3661 tacctcccaa atattttatc taagcatttt ctatttctct tacaatatta atagctgatt
     3721 ctcttggatc ttttgctttt gttataggtc ttccaacaac taaataatga gcacctcttt
     3781 ttattgcatc tgatggagtc acaactcttt tttggtcccc aacctctgct gatttaggtc
     3841 ttattcctgg gcaaactgtt ataaaatcag ttccacatgc ttctactatt ttatctactt
     3901 cttgcgctga acaaacaacc ccatctattc ctgattcttt tgccagttta gctctcttta
     3961 tagctagctc ttctgtcgtc atattacatc ctatgtcctg taaatcttgg ttgcttaatg
     4021 aagttagaac tgttactcca actactaatg gcttttctat atttaatctt tctgcttctt
     4081 ctttagcaac tagtgcacat tgtctcattc cttccatgtc tgatacatgt attgtcatta
     4141 accaaacatt atctctgact gcagctctca ctgcactttt catagtattt ggtatatcgt
     4201 ggaatttaag gtctaaaaat atttttttgc ctttttcttt aagataatct atcgttttac
     4261 cttttgtggc aacatattgc tctaacccta ctttaaatat atctacacta tcttctaatt
     4321 tgtctattag ttctttagct ttatcaaatt cgtcagtatc aattgcaact atcaactttt
     4381 ctttaccatt tatcattttt acatctcctt tttaataaag cctaaatctt cataaaaaaa
     4441 tcctcacacc aaaagaggca caaggatttt atctatacat aactctattc attagctatg
     4501 agatatcttt cccagtctct ctggactagt ttaaaagact ttattatatt taaattttta
     4561 ttttatactc atcaaaaacc tctagaagat ttactagagg ttatatgcta acactatcta
     4621 taacccttta gtctctctgg actactttaa ggaatttagc tgtaatattt ttctttattt
     4681 atataatttt atgtatctat aagataactt aaagttttat cttttttata attaaaccac
     4741 taattgcttt ttatgtcaat aagtctttga attattcttc atctttatca aacaattttt
     4801 tctcaaaata tgattctgca ttatatattg cagcaactgg attatgccca gttactctat
     4861 ctttgacaac taaagttgtt ataggagctt ccatatattt catggctaaa gtatcatgac
     4921 caacacataa tccaagaatt acattaaggt caaccctagc ttcatttaaa aataaagctt
     4981 gtcctattgg attgcacatt acctcatctg aacattttga tattgtttga gatgcttcta
     5041 gtccaattgt actcttaggt attgccccat ttttacatat aacagaaaat acttcaaacc
     5101 catgatgttc aaatactttt tgtactgttt gcatttctct ttttagccca atacaaaatg
     5161 ctataccaat ctttttataa ccacactttt tagcaaactc tataatctct tttagtctag
     5221 tatattgaca atacccctct gcttcaacta gtccagcatt ataagctatt tttttattct
     5281 cttcttcttc ataaagttgc tttgcttttt gttgtagctc aatttctcta gttggacata
     5341 tcttcattaa tttatccata tttccattag aacagcctct aagattacat tttccacact
     5401 tatacatatc caatatcccc ttccaaaatt ttattcatct tgtaattctt ctttttctag
     5461 acttacattt tcttcttgga tttcttcttc tggtttcaca tgctcaacta cagttataaa
     5521 atatgatttt cctattttat tccttatagg aagtttttca tatgaaacat ctattttatt
     5581 ttttatacaa gctatatcaa aatgtcttaa ggctacacct aagctaacct tattcatatc
     5641 ttcacagtgt ttcttaggtt tcttttcaaa taaatgaaga cctcgttcat ctttatggaa
     5701 ataccaaggt tgagaatttt ttacagatgg agctcttctt gtcaattcta aaatatcttt
     5761 ccactttcta ttcctattta tcttatttat catatctttt actggctttc tatctggtct
     5821 agcatatcta gtcctaaaca actcttcatt ttttgctgga tatccaaatg ctattattat
     5881 ataaggattt tctattttgt tttcatcttc ttcttctgtt tttattccaa ctaaataatc
     5941 taagtcatct tcctcatctt cttcaaagct cttattccca atatctacaa attccaagat
     6001 atcttctctc tttaagtttc cttcaagcca acaggtagct attcccaatg ttgtaagacg
     6061 taaaactaca ccttctgtag caaaccctat attctgtaaa tagtcctcac ctttatctga
     6121 tgtgactact atataatgag gtgcctttac cttacattcc ttacccatca aaaaatgtat
     6181 tatatgacct ctatcaacaa catgtgcttt aatatttaaa tcttcattta agtattctaa
     6241 atttttacaa cttcttttta tttcttccat taatggcttt tttatcttct tactggaaaa
     6301 atttcttaca gattttctat aaaaaatggc ttcataaagc tccatttatg ccctcctaac
     6361 tgaactcttc tgacctataa actctataaa tttatataaa tactttatat tttttatagt
     6421 tttatttcct ttttcctcct gctctatttt gaaattcata agaatatcta ctctagttta
     6481 atatagcagt ctaaaggctt aaattctatc ttgtcaatgt ctatatatta gcacaatttt
     6541 tttatttgat tctctatgct aatttaaata acaacactag ctattacttt tataattaag
     6601 ctttgccaga aaaatttttt ttcatttata aatatctatc tcattattat attataagtt
     6661 tttttcggta ttcttgtata gcttttcact aaaaaagtat ttttatttct actttaatac
     6721 tattatcaac aaaaaacata ctgcttaaat aggtttgcta tcttcacaat ctgttttaat
     6781 taaaatatat ttttttattt ctcaatctat aaagtataaa tattttgaat atgacctaaa
     6841 atctaagtaa agttataata ttttataagt tgttcaatat taaaaacata agtaatgtta
     6901 taatacttta taagttattc aatattaaaa atataggagg ataaatatga atttaaatag
     6961 aaatttagaa aaatacgctg aattagcaat aaaagttgga gttaacatac agccaggtca
     7021 aattctactt ataaaatcac caatagaatg tgctgatttt gcaagaaatg cacttaagga
     7081 agcctataaa tgtggtgcta aaaatgtgta tatagagtgg tctgacgaag aaagcactct
     7141 tattaagtac ttatatgctc cagatgaggc atttcatgag ttcccaaagt ggacagcaga
     7201 acaatatgta gatatagcaa aagaaggtgg agcattttta tcagtctatg cccaaaatcc
     7261 agatttatta aaagatgttg accctgaaag agttgcaaat tttcaaaagg catctggaaa
     7321 agctttaaaa gaatggcgtt cttacactct aagtgataaa tgtaaatgga gtatagtctc
     7381 tgtgccaaca aaagattggg caaaaaaagt gtttccaaat ttatcagaag ataaggcaat
     7441 agaaaaattg tgggatgcta tatttaaatg ttcaagagta gatggtcaag accctataaa
     7501 agcatgggaa gaacataata aaaaccttaa atctaaaatg gactttctaa ataaaaataa
     7561 ctttaaaact ttaaaatata aatcctcaaa aactgatttg actttagact taccagaggg
     7621 tcatgtctgg ctaagtggag catccaaaga ccctaatgga gtaagtttta atccgaatat
     7681 tccaactgaa gagatttttg gaatgccaca taagtttaaa gtaaatggaa ctgtttatag
     7741 tacaaaacct cttgtttatg gtggaaacat aattgataat ttcttcttaa catttaaaga
     7801 tggaaaaata atagattttt ctgctgaaaa gggactagat acactagaaa aacttattga
     7861 aacagatgaa ggttctcatt atttaggaga agttgctctt gttccataca actcaccaat
     7921 atccaatacg gatataattt tttataatac tctatatgat gaaaatgcat cttgtcactt
     7981 tgcaattggt tctgcttata aaacttgtct agaaggtgga aacaatttaa aagatgaaga
     8041 attagataaa tatggtgtaa atgacagttt aacacatgta gattttatga ttggttctgc
     8101 tgatatggat attattggtg aaacttatga tggaaagcaa atacagattt ttaaaaatgg
     8161 tgattgggct ttttaacata tgattttaac taaaaagggc tgttggaagt tataccagac
     8221 ttcccagccc tttttaatgt tgatgataaa tattttttat attaattaat ctcattatta
     8281 tagattaatt cctattgttt taaacataag tatctaaaac ttatatttat attatatata
     8341 aaatatccta tttagtaaaa cctttatttt aatttacatg ctaaaaattt aactttgcta
     8401 tttcaacagc agactttgca tcctttgaat aataatcagc gcccatttct tcagcgtatt
     8461 cttctgttaa aactgcaccg cctacaaata cctttgcatt tatctcatta tctctcagtg
     8521 cctgaattgt atctttcata ctttgaacag ttgtagtcat aagtgcactt aatcctacta
     8581 attttatatt tcttttttta gcaacttcca ctacttcttc aatagggaca tctttaccta
     8641 aatctataac ctcatatcca taattttcta gcattatttt tactatattt ttcccaatat
     8701 cgtggatgtc tccttttact gtagcaacta ttatttttcc tttagataca ttattgctac
     8761 ttttacttaa taaagtttcc tttattgtat ttaatgaaac ttttaccgtt tctgctgatt
     8821 gaatcaattg tggtaaaaat atatctccac tatcatatct ttttcctact ttatcaagag
     8881 caggaattaa aatcttatct aatatataat tttcatcctt ctcttttaaa agattcagtg
     8941 ttacattttt tgcttcatct tttaatcccc tttcaactaa aatatccaag ggcaaatctt
     9001 tgtcaatctt tgtagatgaa tccaattttt ttgaattatt tatattacta tattgattta
     9061 tatagtttat acatccttta tctgtgttat ttaatatttt aaaagaattg atagcttcca
     9121 tcatactaca ttcatttgga tttataattg ctaaatcaag ccctgctcct aaagctaatg
     9181 ttagaaaaga agcattaagt gcttttctat tcggaagacc aaatgaaata tttgaaacac
     9241 ctaatatagt ttttactcct aatgtcttta ccattgctat ggcttttata gtttctattg
     9301 cttcttcttg ttgtgctgaa acagttaatg ataaacagtc tataaatatg tcttttggtt
     9361 ttatgccata agaaactgct ctatcaacta tcctttttgc tatttcaaat ctttcttcag
     9421 cctttttagg tatccccttt tcatcaagtg taagtccaac tacacacgaa ccatactttt
     9481 taactatcgg taatatgctt tttaaagact cttctttgcc atttacagag tttactattg
     9541 ttctaccatt ataatatcta agaccttgtt ctaaagcctc tacattggaa gaatcaactt
     9601 gtaaaggtgt atctactact gcttgaatct ctcttattaa ttttggcatt aatttttttt
     9661 catcaatctc tggaagacca acattcactc caagtatttg tgctccagca tttacttgtt
     9721 ctaagcctaa atttatagca taatcaacat tttcattttt taatgcttct tgtagagctt
     9781 ttctacctgt aggatttaat ctttctccaa caactgtagg tgattctact tcaacaaatt
     9841 ttgatggtga acaaactaca catttatcaa ttttctcgat acatcccttt gtaacagaat
     9901 ttatattctc agatatttct tttataaaac ttggattcgt accacaacat ccgcctatta
     9961 tagtagcacc aacctcaacg aactttttaa ccccttcaaa aaattctttt gcatcaacat
    10021 catatatagc ctctccatca actatatttg gaagacctgc atttgcttga accataacag
    10081 gtactgtagc tcttgatgct attttttcaa ctattggtaa aagttgttta ggtcctaatg
    10141 aacaattaac tcctattgca tctacaccta acccttctat agtagccacc atacattctg
    10201 gcatacatcc agtaaaactt cttccacctt catcaaatgt catagtacag aaaataggta
    10261 aatcagaatt ttcttttgct gctaatacag ctactttagc ttcatataaa tccatcatag
    10321 tctctattac tatcacatct actccagatt taacgccttg cagtacttgc cttttaaata
    10381 tttcatatgc cttatcaaaa cttaaagtac ccataggctc tagcatctct cctataggac
    10441 caatatctaa agctacatat cttggtttag tattatctac attttctata gctttcctag
    10501 ccaccaaaac agcattatca atcacttctt ctacagtata ctcactatct aattttagtt
    10561 cattacatcc aaaagtattt gtaagaatta catttgaacc tgcctctaag tatgctgtgt
    10621 gtatttctat aagtttctct ggattttgaa taccaaatac ttctggattt tcacctagct
    10681 ttaacccttt ttgttgaagc attgttccca ttgcaccatc gaatattaat atattatttt
    10741 tcagatattg tcttatttcc acaagagttc gctccttttc tatatgtaca gtttctatag
    10801 tttttacaat tttcacacga cttttctttc ttagatttat ttattcttgt atcagaaata
    10861 cctataatag ccactaccga ctttcttggt atcataattc cattagaagt aattgttaga
    10921 cctatttgtt tttgagcatt taaaacgttt aatatatcta tatttttttc tattggtaaa
    10981 tctccatatc ctggactata tcgcatagtt ataaatttat tttgttttaa aaaaaccttt
    11041 tctacattat cttgaacaat gtcacacact tcttctattg ctgttgttcc acatgaatca
    11101 attattagtc cttttgttaa atctgaataa gagtgtcgcc ttatttcttt ttctatatct
    11161 atccctatag tagctgccat aattacacat tcattacatt cttttaaaag ttcatataca
    11221 tctttactct ctaaagttaa atttgttccc tctaattcta caatattatc tttatttatt
    11281 ctaataggat aaatcctaga taaatatcta ggatttattt tatttttagt tatagctaca
    11341 cattcatcaa tagtatcttt aaggtcttta tctatctctt gacctctgta ttgtaaatat
    11401 cttaaaactt cactttgatt tatgcttata tcttctcttc caattaaatt attctccata
    11461 tatatttaaa gaaaatcctt tatctaaaac atcttttgca tattcctttg ctccaaataa
    11521 agataagtct ctgtagtttc cacattctat agctgtagct gctggcattt tatctgattc
    11581 aagaactttt tttaatgctt cttctaaacc aatcttaact gttttggcat ctacatctcc
    11641 ccatagtatt aaataaaatc ctgttctaca tcccattgga gataaatcta ttacaccatc
    11701 taatgtatct cttaaatagg tagccaaaag atgctctaaa ccgtgcatag cagcagttcc
    11761 aaaactttct acatttggct gtaaaaatct caagtcaaat ttagttactt tatctccctt
    11821 tactccatca agtacactac actttcttac aaaaggagct tttacttttg tatggtctag
    11881 cttaaaactt tctacttttt ccattattta atcctccatt atactttaaa atttgttaca
    11941 ttaattctaa aaaaaactct tcctatctat tattgataag aagagtatat tatttcaagt
    12001 catctcatcg ttgttagcag gaccacctga taaaaggttg gtataggatc actgagctaa
    12061 ctctctcccc taactcttga taagtatctt attaaactga cttaaattat atattacttt
    12121 tttatttttg taaacacttt attgttataa tatttatatc acgtaatcgt acttttattt
    12181 tataactaag ctgattgtat tattggcagt tgtacctcaa atacagtttg atatttatca
    12241 cttgaaacag taatttctcc accatgtaaa tttacaatat tttttgtaat tgcaagaccc
    12301 aacccagaac ctccaacatt tctatttctc gatttttcaa ctctataaaa tctatcaaat
    12361 atatttggta aatctaacac aggaattggt tctccataat ttatcacttt tacaaccgct
    12421 atatctccct ttttctctgt aactatatct acataaagtc catcttttcc atattttaca
    12481 gcattagtta taaggttttc aaatgctctt actaatttat tcggatcagc ttttacaatt
    12541 aatttttctt cattaaaatt aattctacta accataaatt cttgtttaaa ataaatttca
    12601 aattgagata ttacctgact taacaattct actaaattta tttctatttt gtttaatttt
    12661 attgtattat tctgcatttt agtcaattca aacaaatcat ttataagaac gttcaaattt
    12721 ttcgattttt catatgcaat atcaacataa tacatcatct gaacttcatc tttatactta
    12781 ccttcttcta taactcctaa ataacccatt atggatgtca aaggtgtcct taagtcatgt
    12841 gacacatttg ttattaaatc tgtttttgtc tgctgagctt gtctctcttc aatagttata
    12901 tttctaagtt gatgaacgat attattgatt ttatatgcta gttcatcgat atcatccttt
    12961 tcgttatctt ttaatatatc taagtcatat tcaatatcta tacttttttc taaattacct
    13021 tcatccatta aatttacagc actcttaatg atttctaatt tttgattttt tctattagtc
    13081 tttcttataa aatctacaat ttgcttattt ctcttgtacg atattatgga ataaattgct
    13141 aatgatgtga ttgtaaataa aaatggataa aataaactct ctcctataag acttctgaaa
    13201 tctcttaact gatatacaag ttgattaatt acagatatcg caccattgaa atataatgat
    13261 tttattatat acatacttgt tacaataagc attgaagtta aagcacagct tattataaga
    13321 tctaatatag gtcgaaacat atgatttaat aagcttttat ttttcaattt tatatcctac
    13381 tccccataca gtatgaatta ttttttctcc atctgtatat ttttcaattt tatcccttat
    13441 tctgctcata tgaaccatta cagtattatt agattggtaa tatttctctt tccacacttt
    13501 ttcaaatatt tcttctgtac tatatactct acctttattg tttgctagta aatataatat
    13561 atcaaattct cttgatgtca ggattatgtc atttccagct actgttacac tatgtttttc
    13621 cttatctata attactgctc caacttctat gacatcttta tcttcattat tagcaccact
    13681 aaaatagtat gctcttctaa tcagtgactt cactctcaca gtaagttcaa gtgggttaaa
    13741 tggttttgtc aaataatcat ctgcaccagt cattatccct tgtattttat ccatgtcttc
    13801 tgattttgca cttaacataa gtattggtat attcaaactt tctctaactt ttctacaaac
    13861 ttctattcca tccattttag gcatcattat atccaatacc attaaatgaa tattttcctt
    13921 ttttaaaata tctagagcct cttttccatc ttcagcttta attacattat atccactatt
    13981 taaaagatat atctctataa ggtctcttat ttccttttca tcatccacaa caagaattgt
    14041 attcttcact tttacacatc ctaacttaat ctttgtatat agttttattc atataaaatt
    14101 atactatatt ttagttagtg taatctataa atttattcat ttctaaattt ccaatataat
    14161 acaacttatt tacatattta tattatttgt tttgtaaata ttcttctaaa gttaatcctt
    14221 ttgaataaat ttcctcggca gcatccttac caacatatct aatatgccat ggctcatatg
    14281 ctgtacctgt tatatcttct ttaccttcag gatatcttat tataaatcca aacttgtgag
    14341 cattttctgc caaccattta gcttcttttg tacttccaac aaaccatctt tctggatttg
    14401 ttaaatcaat acaaagtcca gtttggtgct cactgtaacc aggctttgct acatatgcat
    14461 ctgccttttc tcgcccctga gattttaccc ttcttgtata agtatccagt tgagtatcat
    14521 aagatctgta tgctgaagac cctaaaaact gtattccttc tgaattagct tgcttaacta
    14581 aatcttcaac tgcctttgca ggttctcctg ccatttgttt ttcttcctct gttgcttctt
    14641 caacaaaagg tatgtttacc ttagtaatat tttcaggagt atagttttta ctaattccat
    14701 ttgtcttgtt taccaataat atatttgata tactatttga gtgattttta ttttgagaat
    14761 ttgcctcaac atcttgtttt gaataatgtt gagcaattac tgttggttct ttatctttct
    14821 taagtaatct atctttagat ataaatacac ttataaatac taccaaaata atagctacta
    14881 ttaatttaaa ttttttcata attaaatatt ctccttttca ttatgtttgt ctataatgtt
    14941 gattttagag tataagactt aagtatttta taagtaaatc ttaaataaat cttaaattta
    15001 atattattaa aggatggtga taaaatgtct atacaaaaac ttctaagtaa agctcgtaag
    15061 gctatacaag attttgatat gatacaagaa aatgataaaa ttgcagttgg actttctggt
    15121 ggaaaagata gtttaactct acttcatata cttaaaagtt atcaaaggtt ttctccacaa
    15181 aactttgaat tgatagccat aacattaaat actggaggtg ttgataattc tccactagat
    15241 aaattgtgta aagagataaa tgttcctttt tatgaatttc aaactgacat aaaagaaatc
    15301 gtatttgata taagaaaaga aaagaatcct tgtgcattat gtgctaatct aagaagaggt
    15361 gctttgaatg ataatgctaa aaaacttgga tgtaataaag tagctcttgg gcatcataaa
    15421 gatgatgcaa ttgagacatt tttaatgtct atgttttatg aaggcagagt aaattgtttt
    15481 tccccaaaga catacctaga tagacaggat ttgacggtta taagacctat gatttacata
    15541 gaagagtata tgactaaaaa aatctcaaaa gattctaact atcctattat aacaaatcca
    15601 tgccctgcaa atggtcatac tagaagagag tacataaaaa atcttattgc taatttaaat
    15661 aaagaaatgc ctgattttaa aagaaatgta tttggtgctc taaataattc tgagaaatta
    15721 tttatatggg ataaagagaa gataaaaaat tttaaatagt aattaaaatt tttaaataaa
    15781 aaagaaagat tgtattaatt gtaaataatc tttatataat ctacacaatc ttactttttt
    15841 atttattata tgttattat
//
LOCUS       DAFNNB010000090        15249 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0091, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000090 DAFNNB010000000
VERSION     DAFNNB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0091"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     misc_binding    28..266
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            334..2973
                     /gene="alaS"
                     /locus_tag="L3M60_002332"
     CDS             334..2973
                     /gene="alaS"
                     /locus_tag="L3M60_002332"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBN6226990.1"
                     /translation="MEKMGLNEIRSKFLEFFESKGHYVANSYSLVPNNDKSLLLINSG
                     MAPLKNYFSGVEVPPSVRMCTSQKCIRTGDIENVGITARHATFFEMMGNFSFGDYFKR
                     ESIKWGWEFVTEWLNIPEDKIWVTVYEEDDDSYDIWAKEMNFPEERMVKLGKDDNFWE
                     IGTGPCGPCSEIYFDRGEEYGCDNPDCKPGCDCDRYLEFWNHVFTQFDRDEEGNYSLL
                     ENKNIDTGMGLERMGCIMQGVDTIFEVDTIKSILEAVEKLTGVKYGENPKNDISIRII
                     TDHIRAVTFLVSDGVLPSNEGRGYVLRRLLRRAARHGKLLGVKELFLQKLIDEVIKVN
                     DKAYPVLVEKESYIKKVVGIEEEKFNETIDQGTEILNSYIEVLKNEGKTVLSGQEAFK
                     LYDTYGFPIDLTKEILEEEHLSVDEEAFNEEMEKQKERARNARGNMDGESWKEDPLSK
                     LESTVDSTFNGYSEIYGEGTIEAIVKDDELVQSAKEGDKVSIVLDNTTFYPEGGGQVG
                     DCGLITNENLVLEVLNTKKGANNSIKHIGIIKSGSISNGDKVKTLVDRETRMSAARNH
                     SATHLLHKALREVLGEHVNQAGSLVTPERLRFDITHFEAISNEELKVIEEKVNNVILS
                     SLDIKCDIMNIKEAKEKGATALFGEKYGDEVRVVSMGDYSTELCGGTHLTNTSQVGMF
                     KILSEGGVAAGVRRIEAITGKAVYEYLKERDGIISEVCVNLKSKEDNLIQRISSLLEE
                     NKNLSKELHDMKAKMSLQSVDSIFDSKVEVNGVNLITNKFEGMDMDTLRETADNLRDK
                     LGSGVVVLANVVDDKVNFVVTATKDVLDKGIHSGNIVREVAKIAGGKGGGRPNMAQAG
                     ASDVSKVDQALSYASEVIKTQVK"
     gene            2998..3255
                     /locus_tag="L3M60_002333"
     CDS             2998..3255
                     /locus_tag="L3M60_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HBN6226991.1"
                     /translation="MEKDLDYTMKFEGIPEDRMSVGDTIDFVYKALVEKGYNPINQII
                     GYLLSGDSSYITSHKNARAIIKKFERDEILEEVITHYLNRK"
     gene            3265..5436
                     /locus_tag="L3M60_002334"
     CDS             3265..5436
                     /locus_tag="L3M60_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6226992.1"
                     /translation="MRKKIMSLFAIVIMIFVSTASYGFAEEKGKVIFIDMNRTNLGSM
                     MNIPILKNEIEKRGYVALMNIRGDQGTDDRRSYASMGAGTRASVTTEDYINFQKVDQD
                     TAKAFEVATGKKAKEINDLGINRSINENLEYGEYGANLGSLGKTLSENGLKTSVIGNA
                     DIIENNQLVKNRNLCLVAMDQYGRIDSGNIDNINKKDSKMPFGISTDYDKLLDETKKS
                     YKNSDAIFVELGDTYRLDMYRLNLNDNTYGTMKKNIVKNINKYLEQVFSMVGENDTVY
                     IASAFPSDLDYKNKRRLSPIIKFSGEGKGLLESATTRKAGIVANLDVGVDILSTFGLE
                     NKEMVGRSYTLVNKEDNVNYLKSLYERIVSISSIRSTIINGFVGVISASWVIAMFAIF
                     FRNYMPHKERVFKVLKEFIKLGIIMPLSFMLAPLMNFKTPISISLGIFATTLILYLLG
                     RKLFKGNDIKHMGFFATLTILVIVIDSVFGTYLMQNNIMSYDAVIGARYYGVGNEYEG
                     VTIASAVFGLAVLLHYKKVSKLYVVIFSLIILITSAYPSMGANVGGAISECVAYLLFI
                     MLIYDIKIDFKKAILLAVSAVVVVFAFAALDIVSGSESHLGMFVQRIFLNGPGEILQT
                     FGRKIQMNMQLAQTSVWVNILLVGIGVIAVLIFKPSRHFRKIMNNFPILFKGFIASMV
                     GCIITLLVNDSGIVAASTASIYILIPLIIISINMIIFEKNN"
     gene            5588..6013
                     /gene="ruvX"
                     /locus_tag="L3M60_002335"
     CDS             5588..6013
                     /gene="ruvX"
                     /locus_tag="L3M60_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBN6226993.1"
                     /translation="MLDGRIMGLDVGDKTIGVAVSDLMGLTAQGVKTIKRVGKKKDIE
                     ELKAIIKEKQVNKIVSGLPKNMNGTLGPQGEKVIKFCELVKEETGIDVEFWDERLSTV
                     AAERSLLEADVSRQKRKKVIDMLAAVIILQGYLDFKINS"
     gene            6091..6378
                     /locus_tag="L3M60_002336"
     CDS             6091..6378
                     /locus_tag="L3M60_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HBN6226994.1"
                     /translation="MEENIINLIDENGVESQFEIILTLEAEGKEYAILMPLDDEEAEE
                     ALIFRIDEDEEGEILIPLESDEEYETVVAVYTAIMEEEGLNYDEDESNGLN"
     gene            6660..7118
                     /locus_tag="L3M60_002337"
     CDS             6660..7118
                     /locus_tag="L3M60_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBN6226995.1"
                     /translation="MANTMDLLKDKLKETGFKITPQRRAIVEILLKHDHSHLSSEEIY
                     DLVRVDCPEIGLATVYRTMQLLDEIGLISKLNLDDGCIRYEISLHKEDCHNHHHLICK
                     NCGKIMEAKEDLLDNIEKEIQSLYKFKILDHDVKFYGLCDECNGVSDSEE"
     gene            complement(7235..7972)
                     /locus_tag="L3M60_002338"
     CDS             complement(7235..7972)
                     /locus_tag="L3M60_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBN6226996.1"
                     /translation="MRGKTHCAIGILTAIQTSLIFKIPISLVDILVSATFAVLPDLDK
                     SNSMVSNFILKNNVSKYIYRIFIYAVNIIIFFISININDNFYLSAIITFVAIIIIEAK
                     LTHTFLRKVFLSLIFILLAICLYIIEVEIYFTIFCLMLSIFPWLKHRSFSHSIFATII
                     VYFLLKQIELITNINNLSFYGTLGYASHMFLGDLFTKQGIPIFYPLSEKKISLGFLTV
                     GGPFSNFIEKSFIFVLIGLIIFSILKI"
     gene            8189..9865
                     /locus_tag="L3M60_002339"
     CDS             8189..9865
                     /locus_tag="L3M60_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HBN6226997.1"
                     /translation="MQLFKKNTNKIKVMALGGLNEVGKNMTVVEYKDEIIVIDAGLSF
                     PEDEMLGVDIVIPDITYLVKNRDKIKGIFITHGHEDHIGALPYILKKINVPVYGARLS
                     IGLIQVKLKEHKMNNVKLNVIGPRQVIKLDNMEVEFLKNNHSIPDAYSIAIHTDQGII
                     YHTGDFKIDLTPIDGDVMDMHRICELSKKGVLLMLADSTNAEKPGFTMSEKTVGIGLD
                     ELFAKGNGRRIIVATFASNIHRLQQIINTAEKFNRKVAISGRSMVNVVGVAKELGYLD
                     ISDDMLIDLNDIYKYEDSELVIITTGSQGEPMSALARMAFSEHKKVEIKSGDLVIISA
                     HPIPGNEKLISRVINFLFEKGAEVVYSDIADIHVSGHACQEELKLIHALVRPKFFMPA
                     HGEYRMLKRHAEIAEQLGMDKENIFVMQTGDVLELDKNSAKVANRIQTGNILVDGLGV
                     GDVGNIVLRDRKHLSEDGLMIVVVTISKDEGKVLAGPDIISRGFVYVRESEDLMDGAK
                     DIIKNVLNECEEKNIKEWAYLKNNIKENLKEYLYQKTKRNPMILPIIMEV"
     gene            10071..10271
                     /locus_tag="L3M60_002340"
     CDS             10071..10271
                     /locus_tag="L3M60_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protein"
                     /protein_id="HBN6226998.1"
                     /translation="MDNVSRQNAIKALKQTKMEIAGEYGMNYEDAFEIIENASNKGVL
                     EGYFKKLEKKKNLGQGISRHLE"
     gene            10325..11488
                     /locus_tag="L3M60_002341"
     CDS             10325..11488
                     /locus_tag="L3M60_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBN6226999.1"
                     /translation="MKKLASTALAILLAFTPLSFSFANNKENADANQLNISSKSAILM
                     DVGSGQILYEKDAHKKLPPASVTKVMTMLLIVEALDSGDIKLDDEVQVSETASSMGGS
                     QIFLEPGETQKVDTLLKGIAVASANDACVAMAEHLAGSVEGFVDRMNAKAKELNMNDT
                     HFANTNGLPVANHYTSAHDIALMSRELLKHEMISKYLTTWMDKVVVGKKQVTVGLANT
                     NKLIKHYQGATGVKTGFTQEAKYCLSASAKRGNTHLVAATLGAETSPERFNDASSLLN
                     YGFANYESVKLCSKGDNIATLTLDKADENKVKLVAKEDLNALIKKGSSKEFEKKIEIV
                     KNPKMPIKKGTVLGKIKICKDKKVIGEVELVNTKDINKASYLQMLQRIIDNMI"
     gene            complement(11698..12120)
                     /locus_tag="L3M60_002342"
     CDS             complement(11698..12120)
                     /locus_tag="L3M60_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2000 domain-containing protein"
                     /protein_id="HBN6227000.1"
                     /translation="METKNSKCVMVIDENLPMGIISNTAAIMGITLGKHAPETVGPDV
                     IDKTGNSHLGIIDIPVPILKGNKEIIKDLRKKLYTLEFEDLTVVDFSDVAQSCNLYEE
                     FTQKIASVPEDELQYFGIAIYGNKKKVNKLTGSMPLLR"
     gene            complement(12141..12977)
                     /locus_tag="L3M60_002343"
     CDS             complement(12141..12977)
                     /locus_tag="L3M60_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6227001.1"
                     /translation="MKKEERTVCFDRELKIEAYRFKGIMQKFPNHFHEHYVIGFIEKG
                     QRYLSCKNKEYTTSTGDLLLFNPFDSHTCEQIDDKVLDYRCINIKPEIMKKTVFEITG
                     KNYLPEFNQPVIFRSELVPLLQELHYIIMEEELDFKKEELFFFLIEQLIEEHTEPNLH
                     SNLENTNIEVQAVCDYLENNYAEHIVLDELSTIAGMNKYSLLRNFTKLKGITPYRYLE
                     NIRVNKAKKLLEKGVEPINAAIQTGFVDQSHFTNFFKNFIGLTPKQYQNIFINDDSNN
                     FK"
     gene            13275..13871
                     /locus_tag="L3M60_002344"
     CDS             13275..13871
                     /locus_tag="L3M60_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HBN6227002.1"
                     /translation="MLSMNIGEIVASLVGIAMAISIHEFGHAYSAHLLGDDTAKAYGR
                     MTLNPVRHVDPIGLLAMFILKIGWAKPVPVNPNNFKNYKIGNLIVSLAGVFCNVLTAI
                     LCVIINKYVHMYAINTIAEYVFLYSIGFAAFNLLPIPPLDGWGVISTFVPYKWNEYMY
                     KFESMSYIILLIALFTGIYRIILTPIYAVLLKIVYLFV"
     gene            13901..14620
                     /locus_tag="L3M60_002345"
     CDS             13901..14620
                     /locus_tag="L3M60_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HBN6227003.1"
                     /translation="MKYNIQLQVYEGPLDLLYDLITKHKIDIKDISIIDITKQYLNYL
                     KMLDKMDLEITSEFITMASKLLEIKSKYLLYKQKDEEEDPRIELMEKLEEYRKFKVAS
                     QDIKENITYVNERFYRNKEEIIIDDNVDLEDISIEAIKNILPYIFKVKTSQIENANDE
                     KLDKIVRKKIISVEEKILYIRDIIKDKIEVTFTNIIKSYENDEIIATFLSILELIKEK
                     EIVVVQDIFFDDILIRKSSEC"
     gene            14622..15161
                     /gene="scpB"
                     /locus_tag="L3M60_002346"
     CDS             14622..15161
                     /gene="scpB"
                     /locus_tag="L3M60_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HBN6227004.1"
                     /translation="MKREDIKYIIESVMFAYGEPISIKELNYIVNKELSSKEIEIMLN
                     LLIEEYREQNRGIQIIKLENKYQMCTNKDYAEYIKKIIEPKKKKSLSQATLETLTIIA
                     YKQPITKVEIEDIRGVKCDKVLQTLFENELIREAGRLNKIGKPIIYKTTDEFLKLLNI
                     ESLEELPPIENYQEVVTNE"
BASE COUNT         6006 a         1608 c         2710 g         4925 t
ORIGIN      
        1 taatataatt agtatattaa ttaaaaaaca aatacagtga taaggaatag taaaatattt
       61 gatgtcttac agagagaggg aaatgctgga agcccttaga atatgatatt tgaagcagcc
      121 ttttagtagc aatcttgaac aatttatctt ttataaagaa tatttaagta gagattgacg
      181 gtagttacgt tatagctaag gttttccgaa gatatcatac tttgtatgat aattagggtg
      241 gtaccgcgat tgtcctcgcc cctataaatt tatttatagg gacgaggttt tttatttaaa
      301 cgaaaaaata aaatttatta ggaggaacat gatatggaaa agatgggatt aaatgaaata
      361 agaagtaagt ttttagaatt ttttgagtca aaaggtcatt atgtagcaaa tagttattct
      421 ttagtaccaa ataatgataa gagtttgtta cttataaact caggtatggc acctttgaaa
      481 aattactttt caggtgtaga agtacctcca agtgtgagaa tgtgtacatc tcaaaaatgt
      541 ataagaacag gagatataga aaatgtaggt ataactgcaa gacatgctac attctttgaa
      601 atgatgggta acttctcatt tggagattac tttaaaagag agtcaattaa atggggatgg
      661 gaatttgtta cagaatggtt aaatatacca gaagataaga tatgggtgac tgtatatgaa
      721 gaagatgatg attcttatga tatatgggca aaagaaatga atttcccaga agagagaatg
      781 gttaaacttg ggaaagatga taatttctgg gagataggta caggtccttg tggtccttgt
      841 tcagaaatat attttgatag aggagaagaa tatggatgtg acaatccaga ctgtaaacca
      901 ggctgtgatt gtgatagata tttagaattt tggaaccatg tattcactca atttgataga
      961 gatgaagaag gaaattatag tttattagaa aataagaata tagatacagg tatgggtctt
     1021 gaaagaatgg gatgtataat gcaaggagta gatactatct ttgaagtaga tacaattaaa
     1081 tctatactag aagcagttga gaagttaaca ggagtaaaat atggagagaa tccaaaaaat
     1141 gatatatcaa taagaataat aactgaccat ataagagccg taacattctt agttagtgat
     1201 ggagtgcttc catcaaatga aggaagagga tatgttctta gaagacttct tagacgtgct
     1261 gctcgtcatg gtaagttatt aggagtaaaa gaattatttt tacagaaact aatagatgaa
     1321 gttataaaag taaatgataa agcatatcca gttttagttg aaaaagaaag ctatattaaa
     1381 aaagtagttg gaatagaaga agaaaaattc aatgaaacta tagaccaagg aacagaaata
     1441 ttgaactctt atatagaagt attaaaaaat gaaggaaaga ctgtcctaag tggtcaagaa
     1501 gcttttaaac tatatgatac ttatggattc cctatagacc taactaaaga aatattagaa
     1561 gaagaacatc tatctgtaga tgaagaagct tttaatgaag aaatggaaaa gcaaaaagaa
     1621 agagctagaa atgctagagg aaatatggat ggagaaagtt ggaaggaaga ccctctatca
     1681 aaattagaat ctacagttga tagtacattt aatggatata gtgaaattta tggtgaagga
     1741 actattgaag ctatagttaa agatgatgag ctagttcaaa gtgcaaaaga aggcgataag
     1801 gtatctatag tactagataa cactactttc tatcctgaag gtggaggaca agttggagat
     1861 tgtggattga taactaatga aaacttagtt ttagaagtat taaatactaa aaaaggtgct
     1921 aataatagta taaaacatat aggtataata aaatctggta gcataagtaa tggagataaa
     1981 gtaaagactc tagtagatag agaaactaga atgtcagctg caagaaatca cagtgctaca
     2041 catttacttc ataaagcatt aagagaagtg ttaggagaac atgttaatca agcaggttct
     2101 ttagtaactc cagaaagact tagatttgat attactcact ttgaagcaat atcaaatgaa
     2161 gaattaaaag taatagaaga aaaagtaaat aatgttattt tatcatcttt agacataaaa
     2221 tgtgatatta tgaatataaa ggaagctaaa gaaaaaggag ctacagcact atttggtgaa
     2281 aaatatggtg atgaggtaag agtagtttct atgggagatt attctacaga actttgtgga
     2341 ggaactcact tgacaaatac ttctcaagta ggaatgttta aaatattatc tgaaggtgga
     2401 gtagcagcag gtgtaagaag aatagaagca ataacaggaa aagctgttta tgaataccta
     2461 aaagaaagag atggaattat ctctgaagtt tgtgtaaact taaaatctaa agaagataat
     2521 ttaattcaaa gaataagttc tttattagaa gaaaataaaa acttatctaa agaattgcat
     2581 gatatgaaag ctaagatgag tttacagtca gttgattcta tttttgattc taaagtggaa
     2641 gtaaatggag ttaatttgat aactaataaa tttgaaggta tggatatgga tacactaaga
     2701 gaaacagcag ataatctaag agataagctt ggttctggtg tagtagtact tgcaaatgta
     2761 gtagatgata aagttaactt tgtagttact gccactaaag atgtattaga taagggaata
     2821 cattcaggta atattgttag agaagttgca aaaatagctg gaggaaaagg tggaggaaga
     2881 cctaatatgg cacaagcagg agctagtgat gtatctaagg ttgaccaagc tttaagctat
     2941 gcaagtgagg ttattaagac acaagttaaa taacattcac tcaaggaggt ttttagtttg
     3001 gaaaaagatt tagactacac tatgaaattt gagggaatac cagaagatag gatgagtgta
     3061 ggagatacta ttgattttgt atataaagca ctagttgaaa aaggatataa ccctataaat
     3121 caaattatag ggtatctcct ttctggtgat tctagctata taactagtca taaaaatgca
     3181 agagctataa ttaaaaaatt tgaaagagat gagatacttg aagaagtaat cacgcactat
     3241 ctgaatagaa agtaggtcaa ataaatgaga aaaaagatta tgtcgttgtt tgcaatagtc
     3301 attatgattt ttgtatcaac tgctagctat ggttttgccg aagaaaaagg caaggtcata
     3361 tttattgata tgaataggac caatcttgga agtatgatga atattccaat cttgaaaaat
     3421 gagatagaaa agagaggata tgtagctctc atgaatataa gaggggatca aggtactgat
     3481 gatagaaggt cttatgcaag tatgggtgca ggaacaagag ctagtgtaac tactgaagac
     3541 tatatcaatt ttcaaaaagt agaccaagat actgctaaag cttttgaagt cgcaacaggt
     3601 aaaaaagcta aagaaataaa tgatttaggt attaatagat ctataaatga aaatcttgag
     3661 tatggagagt atggagcaaa tttagggtca ttaggaaaaa ctttatcaga aaatggttta
     3721 aagacatctg taattggaaa tgctgatata attgaaaata atcaattagt taagaataga
     3781 aatttatgtc tagtagctat ggaccaatat ggaagaattg attcaggtaa tattgataat
     3841 attaataaga aagattctaa aatgccattt ggaatatcta cagactacga taaattacta
     3901 gatgaaacta agaaatccta taaaaatagt gatgctatat ttgtagaact tggagataca
     3961 tatagattag atatgtatag attaaatcta aatgataata cttatggtac aatgaagaag
     4021 aatattgtaa aaaatataaa taaatatctt gaacaagtat tttctatggt tggagaaaat
     4081 gacactgttt atatagcaag tgcatttcca agtgacctag attataagaa taagagaaga
     4141 ctttctccaa taataaaatt tagtggagaa gggaaaggtc tactggaatc tgccacaaca
     4201 aggaaggctg gaattgttgc caacttagat gttggagtgg atatactaag tacatttggt
     4261 cttgaaaata aagagatggt aggtagaagt tatactttag taaataaaga agacaacgtt
     4321 aattatctaa aatcacttta tgaaaggata gtgtctatat cttcaataag gtcaactatt
     4381 ataaatggct ttgtaggtgt aatatctgct tcttgggtaa ttgcaatgtt tgcgattttc
     4441 tttagaaatt atatgccaca taaagagcgt gtattcaaag tacttaagga gtttataaaa
     4501 ttaggcataa taatgccact atcatttatg ttagctcctt tgatgaattt taaaactcca
     4561 atttcaatat ctttgggaat atttgcgact acgctaatat tatacttgtt aggtagaaaa
     4621 ttgtttaagg gcaatgatat aaaacacatg ggattttttg caacacttac tattttagtc
     4681 atagttatag attctgtatt tggaacatac ttgatgcaaa ataacataat gagttatgat
     4741 gctgtcatag gagctaggta ctatggtgta ggaaatgaat atgagggtgt aactatagca
     4801 agtgcagtat ttggcttagc agtattatta cattataaga aggtatctaa attgtatgtt
     4861 gtaatttttt cgttaataat acttataact agtgcatatc cttcaatggg tgcaaatgtt
     4921 ggtggagcta tatcagaatg tgtagcatat ttgttgttta taatgcttat atatgacatt
     4981 aaaatagatt ttaagaaagc tatattatta gctgtttcag ctgtagttgt tgtttttgca
     5041 tttgcagctt tagatatagt ttcaggtagt gaatctcatt taggtatgtt tgtacaaaga
     5101 attttcctaa atggaccagg agagatatta caaacatttg gaagaaaaat tcaaatgaat
     5161 atgcaactag cacagacaag tgtatgggta aatattttac ttgtaggaat tggagtaatt
     5221 gcagtactta tatttaaacc atctagacac tttagaaaga taatgaataa ttttccaatc
     5281 ttgtttaaag ggtttattgc atctatggtt ggttgtataa tcactctttt agttaatgat
     5341 tccggaatag ttgctgcatc tacagcatct atatatatac ttataccact aataataata
     5401 agtataaata tgataatatt tgaaaaaaat aactaaaaaa ttttttactg ttcatataga
     5461 atttaaataa aaaatgcaat atgattatta ctgtaaactg tatgcttaca tagaaaatca
     5521 tattgcaatt tttatgattg ataatatgta tttaatctat ggaatataaa tagataaagg
     5581 aggaattatg ctagatggaa gaataatggg tcttgatgtt ggtgataaaa ctattggagt
     5641 tgctgtgagt gatttgatgg gactaactgc acaaggtgta aaaacaataa aaagggttgg
     5701 taagaaaaaa gatattgaag agctaaaagc aataataaaa gaaaagcagg taaataaaat
     5761 tgtatcagga ttgccaaaaa atatgaatgg aactttaggc ccacaaggag aaaaagtaat
     5821 caagttttgt gagttggtaa aagaagaaac tggtattgat gtagaatttt gggatgaaag
     5881 actttccaca gtagcagcag agaggtcttt attagaggca gatgtcagta gacaaaaaag
     5941 aaaaaaggtt atagatatgt tggcagcagt aataattttg caaggatatt tagattttaa
     6001 aataaattca taaaagaact ataaaattga atacttatga tatataatat agataataaa
     6061 aataaaaata aatttgaggt gaatgacatt atggaagaaa atataataaa tttaatagac
     6121 gaaaatggcg ttgaaagcca atttgaaata atattaacat tagaagcaga aggaaaagag
     6181 tatgcaatat taatgccttt agatgatgaa gaagctgaag aagctttaat ctttagaatt
     6241 gatgaggatg aagaaggtga aatcttaata cctttagaaa gtgatgaaga gtatgaaact
     6301 gttgtagctg tatatactgc tataatggaa gaagagggtt taaactacga cgaagatgaa
     6361 tcaaatggac ttaactagat aaataattaa gtttgtatat aaaaatcagg tatttattat
     6421 atttgcttta attaaacctt attaattgta aaagcactta tagtatatat ctgttttttt
     6481 gttttttatt aaaaatatca agaaatttat gaattttggt gaataatata tcttttatgg
     6541 gtacatattt atattatgag aataaattaa atttatttaa atgtcttgac aaaaaatgaa
     6601 ttatcaatta caatattatt taataaagat tatcaattga aaatagtaag ggtgatgtta
     6661 tggcaaatac aatggattta ttaaaagaca agttaaaaga aactggtttt aagattacgc
     6721 cacaaagaag agcaatagtt gaaatattgc ttaaacatga ccattcacat ctaagtagtg
     6781 aggaaatcta tgatttagtt agggttgact gtcctgaaat aggattagct actgtatata
     6841 gaacaatgca actattggat gaaatagggt taatatcaaa actaaactta gacgatggat
     6901 gtataagata tgagattagt ctacataaag aagactgtca taatcatcat catcttattt
     6961 gtaaaaattg tggaaagata atggaagcta aagaagacct tttagacaat atagaaaaag
     7021 agatacaatc attgtacaaa tttaaaatac ttgatcatga tgttaaattt tatggattat
     7081 gtgacgagtg taatggagta agcgactcag aagaataaac ttctattgct taatgtcaat
     7141 ggaagaatag tatagaactt ttatattcta tgctattctt ccatttttta ttataagcaa
     7201 tctataaagt gtaaatatat agtttttaaa ctaattatat ttttaatatt gaaaatataa
     7261 ttaatcctat aagtacaaaa atgaatgatt tttctatgaa attactaaat ggtcctccaa
     7321 cagttaaaaa tcctaaagaa attttctttt cgcttagtgg ataaaaaatt ggtattcctt
     7381 gctttgtaaa taaatctcct aaaaacatat gacttgcata accaagcgta ccataaaaag
     7441 ataaattatt aatattagtg attaattcta tctgttttag taaaaaatat actattatag
     7501 ttgcaaatat gctatgagaa aaacttctgt gctttaacca tggaaaaatt gacaacatta
     7561 gacaaaaaat tgtaaaatat atttcaactt ctatgatgta taagcatata gctaatagta
     7621 taaatattaa tgatagaaaa acttttctta gaaatgtgtg ggttagtttt gcttcaataa
     7681 ttataattgc aacaaatgta attattgcgc ttaaataaaa attatcattt atatttattg
     7741 aaataaagaa aataataata ttaacagcat atataaaaat tctatatatg tatttggata
     7801 cattgttctt tagaatgaaa tttgagacca tagaattaga tttatccaaa tcgggcagta
     7861 cagcaaaagt agctgaaact aaaatatcaa ctaaagatat aggaatttta aatattaaag
     7921 atgtctggat agctgttaag attcctattg cacaatgagt tttaccacgc ataaatcctc
     7981 ctaaattatc cgatattaaa tcatataata tttagaggat atcaaaataa aataggtttg
     8041 tcaaaataat acgttaaatg tattttaaat atagcttaaa atatgatacg aattatggta
     8101 caataagaat aactggtagc gagtgcgtga tttgatatta tttatgattg tagaacgtgt
     8161 taaaaaatta aatagacaga aggagatgat gcaattgttc aaaaagaaca ccaacaagat
     8221 aaaggtaatg gccttgggtg gacttaacga agttgggaaa aatatgactg ttgttgagta
     8281 taaagatgaa ataattgtta tagatgctgg acttagtttt ccagaggatg aaatgttagg
     8341 agtagatata gttataccag atataactta tttagtaaaa aatagggata aaataaaggg
     8401 tatatttatt acacatggac atgaagacca tataggagct cttccatata tattaaagaa
     8461 aataaatgta cctgtgtatg gagcaagact tagtataggt cttatacaag taaaacttaa
     8521 agaacataag atgaataatg ttaagttaaa tgttataggt cctagacaag ttataaagct
     8581 tgataatatg gaagtagagt ttttgaaaaa taaccatagt ataccagatg cttactctat
     8641 tgcaatacac acagaccaag gtataatata ccatacagga gattttaaga ttgacttgac
     8701 tcctattgat ggagatgtta tggatatgca tagaatctgt gagttgagca aaaaaggagt
     8761 tcttttaatg ctggctgata gtactaatgc agaaaaacca ggatttacta tgtctgaaaa
     8821 gactgttggg ataggtcttg acgaattatt tgcaaaagga aatggcagaa gaattatagt
     8881 tgctactttt gcatccaaca tacacagatt acaacaaata ataaatactg ctgagaagtt
     8941 taatagaaaa gttgctatat caggtcgctc aatggtcaat gtagttggag ttgcaaaaga
     9001 attaggatat ctagatattt ctgatgacat gcttatagat ttaaatgata tatataagta
     9061 tgaagatagt gaattagtga taataacaac tggttcacaa ggggaaccta tgtcagctct
     9121 tgctagaatg gctttttcag agcacaaaaa agtagaaata aagagtggcg atttagttat
     9181 aatatctgct catccaatac caggaaatga aaaattaata tcaagagtaa tcaatttctt
     9241 atttgaaaaa ggagcagaag tagtatacag tgatatagct gatatacacg tttctggaca
     9301 tgcctgtcaa gaagaattaa aacttataca tgcattagtt agaccaaagt tttttatgcc
     9361 agctcatggt gaatatagaa tgttaaaaag acatgctgaa atagctgaac agcttggtat
     9421 ggacaaagaa aatatttttg tcatgcaaac aggagatgtt ttagaattag ataaaaattc
     9481 tgctaaggta gcaaaccgta tacaaacagg aaatatatta gttgatggtc taggagtagg
     9541 cgatgtaggt aatatagttt taagagatag aaagcattta tcagaagatg gtcttatgat
     9601 agttgtagtt actatttcta aggatgaggg taaagtttta gcaggtccag atataatttc
     9661 tagaggattt gtatatgtaa gagagtcaga agatttgatg gatggtgcga aggatataat
     9721 taagaatgtt ctaaatgaat gtgaagagaa aaatattaaa gaatgggctt atttaaagaa
     9781 taatattaaa gaaaacctaa aagaatattt atatcaaaaa actaaacgaa atccaatgat
     9841 acttcctata ataatggaag tataatataa aaaactataa atttgaaaat tagacctctt
     9901 gtaattttta aagtaatatc aaaaatccaa ttgatattat cttaagaatt acaagaggtc
     9961 tttttagttt ttgatttaga aaatatgtat attacataaa ataccaatta taacaatctt
    10021 ttattagtaa aaactactta tatataaatt ttactgatag gaggattctt atggataatg
    10081 tttcaagaca aaatgctata aaagctttaa aacaaacaaa aatggaaata gcaggagagt
    10141 atggaatgaa ttatgaagat gcttttgaaa taatagaaaa tgcatctaat aaaggtgttt
    10201 tagaaggtta ctttaaaaag cttgaaaaga aaaaaaattt aggtcaaggt atatctaggc
    10261 atttagaata aaaaaagtta aatgtgagaa aataagttta aataaacttt aagaggtgac
    10321 atcgatgaaa aaattagcta gtactgcttt ggctatatta cttgcattta ctcctttgag
    10381 tttttctttt gcaaataata aggaaaatgc tgatgccaat caactaaata tatcttcaaa
    10441 atcagcaatt ttgatggatg ttggtagtgg acaaatttta tatgaaaagg atgcacataa
    10501 aaagcttcct cctgccagtg ttacaaaggt aatgacaatg ttacttattg tagaagcttt
    10561 agattcaggt gatattaagc ttgatgatga agttcaagta agtgaaactg cgtctagtat
    10621 gggtggtagt cagatatttc tagaaccagg agaaactcaa aaggttgata cgttattaaa
    10681 gggtatagct gttgcttcag caaatgatgc ttgtgttgct atggctgaac acttagctgg
    10741 aagtgttgaa gggtttgttg atagaatgaa tgctaaggca aaagagttaa atatgaatga
    10801 tactcatttt gcaaatacaa atggacttcc agttgctaat cactatacat ctgctcatga
    10861 tatagcacta atgtctagag aattgttaaa acatgaaatg ataagtaaat acttaactac
    10921 atggatggat aaggttgtag tagggaagaa acaagttaca gtaggtcttg caaataccaa
    10981 taaacttata aaacactacc aaggagctac tggtgttaaa actggtttta ctcaagaagc
    11041 aaaatactgt ttatcagcat ctgcaaagag aggtaatact catctagttg ctgcaacttt
    11101 aggagcagag acatctccag aaagatttaa tgatgcatct agtcttttaa actatggatt
    11161 tgctaattat gaaagtgtta agttatgtag taaaggcgat aatatagcaa cacttacatt
    11221 ggataaggct gatgaaaaca aagttaaatt ggttgctaaa gaagatttaa atgctcttat
    11281 taaaaaagga agctctaaag aatttgagaa gaaaattgaa atagtaaaaa atcctaagat
    11341 gccaattaaa aagggaactg ttttaggaaa gataaagata tgtaaagata aaaaagtaat
    11401 aggcgaagtg gaattggtaa atactaagga tataaataag gcaagctatc tacaaatgct
    11461 tcaaagaata attgataaca tgatataaat aattatcaag tgaaatataa ttataatata
    11521 caaagatgta attataatac acataaaaaa tacccttatg taattatttt ccataggggt
    11581 atttttattt actgtatata agtattttat aatcttatga ctttatataa atatagaaaa
    11641 gtttatgtat catacaatat catttgattt aatatcatat acatgaatga ttaacactta
    11701 tcgaagtaaa ggcatactac cagtaagttt atttactttc tttttatttc cataaattgc
    11761 tataccaaaa tactgtaact catcttctgg aacactagca atcttttgtg taaattcttc
    11821 ataaagatta caactttgtg ctacatctga aaaatctaca acagttaaat cttcaaattc
    11881 caaagtatat aattttttac gtaaatcttt tattatttct ttgtttcctt ttaaaatagg
    11941 tacaggaatg tcaataattc ctaagtgact attacctgtt ttgtcaatta catctggtcc
    12001 aacagtttct ggagcatgtt ttcctaatgt aattcccatt atagctgctg tattagatat
    12061 aattcccatt ggtaaatttt catctattac catgacacat tttgaatttt tagtttccat
    12121 ttttatttct cctttttacc ttatttaaag ttatttgaat cgtcatttat aaatatattt
    12181 tgatactgct ttggtgtaag accaataaaa ttcttaaaaa agtttgtaaa gtgactttgg
    12241 tctacaaatc ctgtttgtat cgcagcattt ataggctcta cacctttttc taaaagcttt
    12301 ttagctttgt ttactcgtat attttctagg tatctatatg gtgtaatacc ttttaatttt
    12361 gtaaaattac gaagtagaga gtacttattc atacctgcta tagtgcttaa ttcatcaagt
    12421 actatatgtt cagcataatt attttctaaa taatcacata ctgcttgaac ttctatattt
    12481 gtattttcca agttagaatg caaatttggc tctgtatgtt cttcgattaa ttgttcaatt
    12541 agaaagaaaa aaagctcttc ctttttaaag tccaattctt cctccatgat tatatagtgt
    12601 aattcttgta gtagaggtac tagctcactg cgaaatataa caggttgatt aaattctggt
    12661 aaataatttt tccctgtaat ttcaaagaca gtttttttca ttatttctgg cttaatattg
    12721 atacatctgt aatcaagtac tttatcatct atttgttcac aagtatgact atcaaaaggg
    12781 ttaaaaagta gcaaatctcc agtacttgtg gtatactctt tatttttaca agataaatat
    12841 ctttgacctt tttctataaa accaattaca taatgttcat gaaaatggtt tgggaatttt
    12901 tgcattatgc ctttaaatct atatgcttca atttttaatt ccctatcaaa gcatactgtg
    12961 cgttcttctt ttttcactta gttaacctcc tttcatgtta taaaaagtgt aacacatatt
    13021 tttttatact tcttgtatga tattgactaa aacataataa tgtcaattca tgtcaatata
    13081 atattacact tacagtagtg taattattat attttcacaa cgaatatgat ataataataa
    13141 aagttataaa ataaaaagca aattttaatt ttatgcctag aagttaaaat tattataata
    13201 taaaataatt ttgattataa gttatttaat tgttataata atattaaaaa atttgattta
    13261 ggagatgtaa attaatgtta agtatgaata taggagaaat agtagcaagt ttagttggta
    13321 tagctatggc gatatcaata catgagtttg gtcatgctta ttcagctcac ttattgggag
    13381 atgatacagc taaagcttat ggtagaatga cattaaatcc tgtaagacat gttgacccaa
    13441 taggtctttt ggctatgttt attcttaaaa taggatgggc aaaaccagta ccagtaaatc
    13501 caaataactt taaaaattat aagataggaa acctgatagt ttcacttgca ggagtttttt
    13561 gtaatgtttt gactgccata ctttgtgtaa ttatcaataa atatgtgcat atgtatgcga
    13621 taaatacaat agctgaatat gtgtttttat atagtattgg atttgcagct tttaatttgc
    13681 ttccaatacc accacttgat ggatggggag ttatatctac atttgtaccg tataaatgga
    13741 atgaatatat gtacaaattt gaaagtatga gttatataat tttattaatt gctttattta
    13801 ctggtatata tagaataata ttaactccaa tatatgctgt actactaaaa atagtatatt
    13861 tatttgttta atataataaa cagattaaag agggattgat atgaagtata atatacaatt
    13921 acaggtctat gaagggccat tagatttact gtatgattta ataacaaagc ataaaataga
    13981 cataaaggat atatctataa tagatataac taagcagtat ctaaattatt tgaaaatgct
    14041 agataaaatg gatttagaga taactagtga gtttataact atggcatcaa aacttttaga
    14101 aataaagtct aaatatctac tttataagca aaaagatgaa gaagaagacc caagaataga
    14161 acttatggaa aagctagaag aatatagaaa gtttaaggta gcatcacaag atataaaaga
    14221 aaatataact tatgttaatg agagatttta tagaaataaa gaagagataa ttattgatga
    14281 caatgtagat ttagaagata tatcaataga agcaataaaa aatatactcc catacatatt
    14341 taaagttaaa acatcacaga tagaaaatgc taatgatgag aaactagaca aaatagtaag
    14401 gaagaagata atatctgtag aagaaaagat attgtatata agagatatta taaaagataa
    14461 gattgaagtt acttttacaa atattataaa atcatatgaa aatgatgaaa taatagctac
    14521 ttttttatct atattagagc ttataaaaga aaaagaaata gttgtagtac aagatatatt
    14581 ttttgatgat attctaataa gaaaaagttc ggagtgttaa aatgaaaaga gaagatataa
    14641 agtatattat agaatctgtt atgtttgcat atggagagcc gataagtata aaagaattaa
    14701 actatatagt aaataaagaa ctttcatcaa aagaaataga gattatgtta aatttactta
    14761 ttgaggagta cagagaacaa aatagaggta ttcaaataat caagttagag aataagtacc
    14821 aaatgtgtac aaacaaagac tatgcagaat atataaagaa gattattgaa ccaaagaaaa
    14881 agaaaagttt gagccaagct acattagaaa ctctaactat aattgcctac aaacaaccaa
    14941 taacaaaggt agaaatagag gatataagag gagtaaaatg tgataaggta ttacaaactt
    15001 tgtttgaaaa tgaacttata agagaagctg gtagactgaa taagataggg aaaccaataa
    15061 tttataaaac aacagatgaa tttttaaagt tgttgaatat agaaagtctt gaagagttgc
    15121 ctcctataga aaattatcaa gaagtggtaa caaatgaata aaaatataaa aacacaacaa
    15181 cttagagaat ttttaacttt gaaagattta aaattctcta agttgttgtg ttttttgtat
    15241 aaatataaa
//
LOCUS       DAFNNB010000091        15064 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0092, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000091 DAFNNB010000000
VERSION     DAFNNB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15064
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0092"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(129..1064)
                     /locus_tag="L3M60_002347"
     CDS             complement(129..1064)
                     /locus_tag="L3M60_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HBN6227005.1"
                     /translation="MKCTNFTFKGEEGLDIYTYKWEDENIKNPKAVVQIAHGMAETAQ
                     RYETFAKVLTKNGYIVYINDHRGHGKTAKIIENVGHLAEKEGFRCLVEDMYTLTNIIK
                     KENEDLPIYLFGHSMGSFASQRYIMDYSNNLSGLILCGSNGRQGIILNLAHMIINREI
                     KKYGRRSRSNKINDLIFGGEIIRRNEKTKFDWLSRDKEQVEKYINDPFCGVVCSCGFF
                     YDLIQGLKEIEDKENLKKVPLDVPIYIISGDKDPIGKNSKGVLRLRDRYIKLGVKDVT
                     CKLYKGGRHELLNEINKEEVFEDVICWLNNKIGIL"
     gene            1346..1893
                     /locus_tag="L3M60_002348"
                     /pseudo
     CDS             1346..1893
                     /locus_tag="L3M60_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            complement(2100..4136)
                     /locus_tag="L3M60_002349"
     CDS             complement(2100..4136)
                     /locus_tag="L3M60_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HBN6227006.1"
                     /translation="MDSNRKPNNLINEKSPYLLQHAYNPINWYSWNEEAFKKAKEEDK
                     PIFLSVGYSTCHWCHVMEKESFEDEEVAEIMNRNFVAIKVDKEERPDVDSVYMTVCQA
                     MTGSGGWPMTIIMTPDKKPFFAGTYFPKYSRYNRPGVIDLLENVSEKWNTSRDILIKS
                     GDEIIEALKDDFGVKNTEGDLSKEMLSSSVRVFKAIYDEKYGGFGNAPKFPSPQNLMF
                     LMKYYSIEKDKDVLKMVEKTLDGMYRGGLFDHIGFGFSRYSTDKKWLAPHFEKMLYDN
                     AMLTIAFLDAYKITNKELYKEIAMKTIDYVVREMQDKDGGFYSAQDADSEGEEGKFYT
                     FNPLEIIEVLGEEDGTFFNNYFDITSSGNFEGKSIPNLIKNKEYERHNEKIDNLSKKV
                     FEYRKERTSLHKDDKILTSWNALMVVALTKAYSTLKNDMYLDYSNKCLDFINNNLVNE
                     SGRLLARYRDGSSDYLAYLDDYAFLIWAYIELYESTFNMKYLEKALNLNESCIDLFWD
                     YEKSGFYIYGKDSENLIARPKDLYDGAIPSGNSVQLYNLIRLAKITGDNKLEEMSYKQ
                     LKLYVNNVKSSPTGYSFYMLSLMFELYSTKEIICIFKEDSDLSAFKELISENFIPNTT
                     FLAKKYNEENTIIGFLNNYKLKEDKTSYYVCQSNSCSQPINNLQKLKDMILGVE"
     gene            complement(4306..4983)
                     /locus_tag="L3M60_002350"
     CDS             complement(4306..4983)
                     /locus_tag="L3M60_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBN6227007.1"
                     /translation="MFKHDKALLKEVKVERPNPQYAVLMQEQLGGANGELKAAMQYLS
                     QSFRIKDPQIKDLFLDIAAEELSHMEMVAQTINLLNGHDVDYNAVNTGEIETHVLTGL
                     SPVLINSSGAPWTANYVTVTGDLVADLLSNIASEQRAKVVYEYLYRQIDDKYVKETID
                     FLLNREEAHNALFRDALNKVKDTGSNRDFGVTEDSKLYFDLSTPGPNHDTKIDVNPPS
                     FEKPLKK"
     gene            complement(5191..6879)
                     /gene="ilvB"
                     /locus_tag="L3M60_002351"
     CDS             complement(5191..6879)
                     /gene="ilvB"
                     /locus_tag="L3M60_002351"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HBN6227008.1"
                     /translation="MNGAKVILECLKKEGIDTIFGYPGGAVIPLYDALYDYSDDFKHI
                     RTSHEQGLVHAADGYARSTNTVGVCFTTSGPGATNAITGIATAFMDSSPMVVISGQVP
                     TSLLGKDSFQEIDITGATLSMTKHNYLVRNTKELVPTIKEAFKVANSGRKGPVLVDVP
                     KDLFLAEMDFSDEDYDLCQIDDYIDYKSDFSLDDEINVKLLNEAIDIIKESKKPVIYA
                     GGGVKSSDSEDILEKFATKIDTPVLNTLMGLGNIDRKNELSLGMVGMHGSRESNLALS
                     NSDLVIAIGARFSDRVISKSSEFAKNAKIIHIDIDPSEISKNIESNVSLIGDVKLVLS
                     LLIERVESKNNSNWKEEIKTFRKSEGIQTDEFHPQSILKKINEKYETLKKPTVVVTDV
                     GQHQMWAAKYWNFKGNKSFITSAGLGTMGFGLGAAIGAKVGNIDKNVVLVTGDGSFRM
                     NCNELATVANYNVPMLILLLNNRTLGMVRQWQKLFSNERYSQTDINENVDYVKLVNAY
                     KIDGYKISNMEELGKVLDVIDFNKPVFLQCDIDKDYDVYPIVAPNDALENLICN"
     gene            complement(6932..7927)
                     /gene="ilvC"
                     /locus_tag="L3M60_002352"
     CDS             complement(6932..7927)
                     /gene="ilvC"
                     /locus_tag="L3M60_002352"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBN6227009.1"
                     /translation="MKMARMYYEKDVDLEVLKNKKVAVLGYGSQGHAHAQNLRDNGVY
                     VVIGLYDGSKSAQKAKEDGFEVKSVAEATKESDLIMMLMPDEKQKKVYEESVKDNLKE
                     GQTLAFAHGFNIHYNQVQPPEFVDVVMVAPKGPGHLVRNVFTKGSGVPALFAVYQDYT
                     KKATETVLAYAKGIGATRAGVLETTFKEETETDLFGEQSVLCGGISELIKLGYKTLVD
                     AGYQKEVAYFECLHEMKLIVDLIYEGGFERMRYSISDTAEYGDYVSGKRVITDAAKQG
                     MQNVLEDIQNGKFAKAWIKENEEGRENFLKTREEEYNTEIAEVGRNLRSMMSFLK"
     gene            complement(8206..8445)
                     /locus_tag="L3M60_002353"
     CDS             complement(8206..8445)
                     /locus_tag="L3M60_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HBN6227010.1"
                     /translation="MENTVYAKLNQGIDSFLRVAMTLRRREVDIQSISMTVDNMNNSG
                     IKLIVNEEKTSLDSVLNHMKKLHDIREITVERMKH"
     misc_binding    complement(8558..8814)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8975..9736)
                     /locus_tag="L3M60_002354"
     CDS             complement(8975..9736)
                     /locus_tag="L3M60_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBN6227011.1"
                     /translation="MNIGSKDSDILNVNFRGIDIKVILVYKNRKNISIKIDPFGKVIV
                     MSPPKMSKKIIKDIIIEKGDWILKKSEKYKGREEVYEQRMFITGEKFLYLGEEYHLVI
                     KEMLKDSVKKSNCKITINEYEIIVQTNDTSTDFIKKSLKSWYKIESERLVLERIDFLK
                     LNYEIMNQLVPASVKVKEQKKRWGSCTSQKNIYINSKISMAKLDVIDYVIIHEFSHLV
                     HMNHSKKFYDLVKSIMPDYKDKENWLKINGYKITI"
     gene            complement(9741..10562)
                     /gene="xdhC"
                     /locus_tag="L3M60_002355"
     CDS             complement(9741..10562)
                     /gene="xdhC"
                     /locus_tag="L3M60_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HBN6227012.1"
                     /translation="MNIYEQAIRLIENNEDFAFATITSHSGSTPRETGAMMIVKNDST
                     IFGSVGGGSVEAECIKHAINVIKNRESMLYKFTLNKSDVAKLGMICGGTGEIQIDFID
                     SKLKSNIEKFNKRLKENTSKAYIFGAGHISRDVAVILSLLEFRTVVIDDREEFANHER
                     FPESEVIVLDSFEEIPDFPTDENSYIIILTRGHLYDSSALEWALKRDAGYIGMIGSRT
                     KIGLTYEKLMKKGFKKEELSRVHAPIGIKLNAQTPAEIAVCIAAELINCRANKEK"
     gene            complement(10604..11167)
                     /locus_tag="L3M60_002356"
                     /pseudo
     CDS             complement(10604..11167)
                     /locus_tag="L3M60_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429797.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
     gene            complement(11395..12264)
                     /locus_tag="L3M60_002357"
     CDS             complement(11395..12264)
                     /locus_tag="L3M60_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HBN6227013.1"
                     /translation="MTSKERLNKLKEEYHKIPIPKKLDTIINHEKIENIYQEKNKKVN
                     RLRFKVAIAFACIFTVLVNISPVFADNFSKIPVIGAIVEVITIKNYSLKSENYEAEID
                     IPKIRGLKDKNLEQRLNSSFMEDGKRLYHQFEERMEKIQSSKNKGHKSLSLSYSVKNN
                     SKKFLSIEMTKNEIEASSYVSKVHYTIDKKRQIVLTLPMLFKDDKYIKVISDNIKEQM
                     REQMKRDSTKFYFIDQKKELPVEDFKTINKYQDFYFNKNEELVICFDEYEVAPGYMGA
                     VEFVIPYKVIKDL"
     gene            complement(12276..12758)
                     /locus_tag="L3M60_002358"
     CDS             complement(12276..12758)
                     /locus_tag="L3M60_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6227014.1"
                     /translation="MFKSLKRTKVEKYIIDNKDSFYRIAYSYTKNEEDALDVVQEAMY
                     KALYSVENIKEVNYIKTWFYKILIRTSIDFIRKNRKYNNMTDIDLVDETGEYDKYTDL
                     DLRRALEELPIEYKSIIILRFFEDLKIEEVAIILDENVNTVKTRLYTALKKLKLKIEE
                     "
     gene            complement(12810..13742)
                     /locus_tag="L3M60_002359"
     CDS             complement(12810..13742)
                     /locus_tag="L3M60_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN6227015.1"
                     /translation="MVGILSLMMVGCNKSLAKVNDVEITKEQYKKTKAVLSATNNYIN
                     GQSLDELEKTLDKKGRNKLENVIISFMVDNELLYQEAKDKGLTPSKSEVDSKYQELED
                     KMNLNTSYKEKMDKAGVDKEYLKQEISRDLAIDKNKKAFEDRINISDNDMEAYYTSHK
                     KDFNVEEISASQILISTLDKNKKEVSKDKKEALKKKADNILTKIKNGESFESLAKKYS
                     DDKATGKNGGQLGYFSKDDKNAEFTKEVFKLKKNEVSKVFETSYGYHIVKVTDKRERQ
                     KSFNECQSLIRESILNEKYIEHIKKLNEDAKIDR"
     gene            complement(13835..14668)
                     /locus_tag="L3M60_002360"
     CDS             complement(13835..14668)
                     /locus_tag="L3M60_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBN6227016.1"
                     /translation="MRNKKKKINRKKLYLLLSAIVVCLAFVVVGARVSSLNMKENEIS
                     RNAKLSSSTITDIVSNTATEVSKGPSKSSGKVAYITIDDGPSKFTDQIIKTLNKYNVK
                     ATFFMIDGNMKEYPQQVKNIIKNGNTAGFHSVSHDIHKLYVTSTSAKEEFDTNDQTFY
                     KITGKHSKVIRIPYGSKPYTPQASYQALVDAGYKIWDWDLDTEDWRSNSSQIIQNVKN
                     HIKNRKGEDKDQLVVLMHEKKQSAEALDSVLKFLSDEGYEFAPVDQNQIPKNYWLRNL
                     E"
BASE COUNT         4749 a         2612 c         1450 g         6253 t
ORIGIN      
        1 ataaaaaggt gtttcattga taataaataa tcatcttgaa acactttctt ttctgtttta
       61 cacatattaa atatactttt caaacattta catagaatta aatttaatat taatataata
      121 ttcctttatc acaatattcc tattttattg tttagccaac aaattacatc ctcaaaaact
      181 tcttctttat ttatctcatt taataattca tgtcttccac ctttgtaaag tttgcaagtt
      241 acatctttta ctcctaactt tatatatcta tcccttagtc ttaagacgcc cttactattt
      301 ttccctatag ggtctttatc tccagatatt atgtaaatag gtacatcaag tggcactttt
      361 tttaagtttt ctttatcctc aatttctttt aacccttgaa ttaaatcata aaaaaagcca
      421 caactacaca ccactccaca aaatgggtca ttgatatatt tctcaacttg ttctttatct
      481 ctacttaacc aatcaaactt agtcttctca tttcttctta ttatttcgcc accaaaaatc
      541 aaatcattta ttttattact tctagaacgt cttccatact ttttaatctc acgattgatt
      601 atcatatgag caagatttag aatgattcct tgccttccat ttgaaccaca tagtataaga
      661 ccagataaat tattgctata atccattata tatctttgac ttgcaaatga acccatgcta
      721 tgaccaaata gataaattgg caaatcttca ttttctttct ttataatatt tgttaaagta
      781 tacatgtcct ctacaagaca tctaaaacct tctttctcag ctaaatgccc aacattttct
      841 attatctttg cagtttttcc atgacctcta tggtcattga tatatactat ataaccattt
      901 ttagtaagca ccttggcaaa agtctcatac ctttgtgcag tttcagccat tccatgcgca
      961 atttggacaa cagcctttgg attttttata ttttcatctt cccatttata tgtatatata
     1021 tctaaacctt cctctccttt gaaagtaaag tttgtacact tcataattat ccctccagat
     1081 tgattttatt cagtatatta atttatatat tatattacaa gtgttttcaa gtcaaaatct
     1141 ttctaaaaaa atttaagaat tattaatgct tcatagaagt tatcattgtg tatagaaatt
     1201 attacataaa attatataat aatttaatta atatttgcat aaaaaagtaa tactttaata
     1261 tattttgata ccatattgac attagaatta attcatgata ttttattttc gacaaaacaa
     1321 catctaaaga taaagtagat atttaataga ttattcaaat taactaaaag aactcttttt
     1381 aatgaatcaa acatatgcaa cttttttacc attacaaaca aaatacaaat agaaggatat
     1441 aaatattttg gtatacttac ttcaagacag tatatgacta ttttatctac tcttcattta
     1501 tcagaagaag aaacaacatt aaataatatt gcaagaaaaa tggtcacaag taaacaaaat
     1561 ataaatagga tagttgttaa tcttaaaaaa aatggatata ttgatgtaat tacaagtcct
     1621 catgataaga gagcaattac tgttaaagtt atagacttag gaaaagaagt tatggtaaca
     1681 tgtggtagag ctgtaataaa cttaatggca gatgtatttc atgaatttat caaagatgaa
     1741 ttggaaacct tgtggaattt actaaaaaat gtattgtttt aataatgaag aacaagacgg
     1801 ctttaaagaa ggtgctaatt ttgggaatat gaagaaataa ataaaattaa atctgaagca
     1861 cttgaagaat ttgtaaaaat aagaaatagg taaataaaaa cgattaatac tataacatct
     1921 ataataatac cactttttta taatgcaata ttttatataa ctataatatc attttcatct
     1981 tttaacaaaa caattattac atagattaag tgttcttaga aactattttt tgttttttgt
     2041 acaaaaaaag gagatgatta gatttttact ttaagacatc tcctttttca ctattctggt
     2101 tattctactc ctaaaatcat atcttttaat ttttgtaaat tattaattgg ttggctacaa
     2161 ctattacttt gacacacata ataactagtc ttatcttcct ttaatttata gttatttaaa
     2221 aaacctataa tagtattttc ttcattgtat ttctttgcta aaaaagttgt atttggtata
     2281 aaattttcac ttatcaattc tttaaatgca cttaaatcgg aatcttcctt aaaaatacat
     2341 ataatttcct tagtagaata aagttcaaac atcaatgaaa gcatataaaa gctgtagcca
     2401 gttggagaac tctttacatt attcacatac agttttaatt gtttatatga catttcttca
     2461 agtttattat cgccagtaat tttcgcaagt cttataagat tgtataattg aacagagttt
     2521 cctgatggaa tagctccatc atacaaatct tttggtcttg ctattaaatt ttcactatct
     2581 ttaccatata tataaaatcc acttttttca taatcccaaa aaagatcgat acagctttca
     2641 tttaaattta aagccttttc caaatatttc atattaaatg ttgactcata aagctctata
     2701 tacgcccaga ttaaaaaagc ataatcatct aaatatgcta aatagtcgga acttccatct
     2761 ctatatctag ctaacaatct acctgactca tttacaagat tattatttat aaaatcaagg
     2821 cacttatttg aataatctaa atacatatca ttttttaaag tactatatgc ctttgttaaa
     2881 gcaactacca tcaatgcatt ccatgatgta agtatcttgt catctttatg caaagatgtt
     2941 ctttcttttc tatattcaaa taccttttta ctaaggttat ctatcttttc attatgtctc
     3001 tcatattctt tattttttat aagattagga atacttttgc cctcaaagtt tccactacta
     3061 gttatatcaa aataattatt aaagaaagtt ccatcttctt cacccaacac ttcaattatc
     3121 tcaagaggat taaatgtata aaatttacct tcttcacctt cactatcagc atcctgcgct
     3181 gaataaaacc caccatcttt atcctgcatc tctctaacca cataatctat agttttcata
     3241 gcaatttctt tatataactc tttatttgtt attttatacg catctagaaa agctatagta
     3301 agcatagcat tatcatatag cattttttca aaatgaggag ccaaccactt tttatcagta
     3361 gagtatcttg aaaacccaaa tccaatatga tcaaacaatc ctcctctata cataccatct
     3421 aaagtttttt ctaccatttt taatacatct ttatcttttt ctatactata gtatttcatc
     3481 aaaaacataa gattctgagg acttggaaat ttaggggcat taccaaaacc accatacttt
     3541 tcatcataaa tagccttaaa tactctaact gatgaactta acatctcttt tgataaatca
     3601 ccttcagtat tttttacacc aaaatcatcc ttaagagctt ctattatttc atctccagat
     3661 tttattagta tatctctact tgtattccat ttttcagata cattctctaa taaatctata
     3721 actccaggtc tattatacct tgaatatttt gggaagtatg ttcctgcaaa aaaaggtttt
     3781 ttatctggcg tcattattat agtcattggc catccaccac tacctgtcat agcttggcaa
     3841 actgtcatat acacactatc tacatcaggt ctttcctctt tatctacttt tattgcaaca
     3901 aaatttctat tcattatttc agcaacttct tcatcttcaa aagattcttt ttccataaca
     3961 tgacaccaat gacatgttga atatcctaca cttaaaaata ttggtttatc ctcttctttt
     4021 gcttttttaa aagcctcttc attccaacta taccagttta taggattata tgcatgctgt
     4081 aaaagatatg gagatttttc atttattaga ttattaggtt ttctattact gtccaaaata
     4141 ccacctcatt tgttatttta ttatatgttt tgcaataaac aaatagatat acacttatag
     4201 taagtgtata cccattttta actatacata tttaattttt tatctaataa actcttctag
     4261 taaataattt cttatgacta ctaaaataac ataaaaaata taaaattatt tttttaaagg
     4321 tttttcaaaa gaaggaggat taacatctat cttagtatca tgatttggcc ctggagtaga
     4381 taaatcgaaa tataacttag aatcttctgt aactccaaaa tctctatttg aacctgtatc
     4441 tttaacttta tttaaagcat ctctaaatag agcattatgt gcttcttctc tattaagtaa
     4501 gaaatctatt gtttctttta catatttatc atctatttgt ctatacaagt actcataaac
     4561 aaccttagct ctttgttcag aagctatatt tgataataag tcagctacta aatcccctgt
     4621 aactgtaaca taattagcag tccaaggagc tcctgatgag tttattaaaa ctggtgacaa
     4681 accagttaat acatgtgttt ctatttcgcc tgtattaact gcattataat caacatcatg
     4741 tccatttaat aaatttatag tttgtgctac catttccata tggctaagct cttctgctgc
     4801 tatatcaaga aataaatctt ttatttgagg gtcttttatt ctaaaacttt gtgacaagta
     4861 ttgcattgct gcttttaact ctccatttgc accacccaac tgttcttgca ttaaaacagc
     4921 atattgagga tttggtcttt ctacttttac ttcttttaat aatgctttat cgtgtttaaa
     4981 catatgaata cctccgtaaa ttattattca aaattattat ttacttaagt tgtaatatta
     5041 ttctaaattg tataattaaa atttgataaa ttcttaatta tatattcatt tttttatcta
     5101 caaaaataaa aaataccact ctatctctaa ataaaaactg aatatcaata aaagtttaat
     5161 aaaaaatttt atcaatattc agtataagtt ttagttacat atcaaatttt ctaatgcatc
     5221 attaggtgct acaataggat aaacatcata atccttatct atatcacatt gtaaaaacac
     5281 aggtttatta aaatctatca catctagtac ctttcctaat tcttccatat tggatatctt
     5341 atatccatca atcttatatg catttacgag tttaacataa tccacatttt cattaatatc
     5401 agtttgagag tatctttcat tggaaaataa cttctgccat tgtcttacca ttcctaaggt
     5461 tctattattg agtagtaaaa taagcatagg tacattataa tttgcaactg tagctagttc
     5521 attacagttc attctaaaac ttccatcacc agttactaaa accacatttt tatcaatatt
     5581 accaacttta gctccaatag ctgcacctaa accaaatccc atagttccta atcctgctga
     5641 tgttataaaa cttttatttc ctttaaaatt ccagtattta gcagcccaca tttgatgttg
     5701 accaacatca gttactacca cagtaggctt ctttaatgtt tcatatttct catttatttt
     5761 tttaagtata ctttgtggat gaaattcatc cgtttgtata ccctcactct ttctaaatgt
     5821 ctttatctct tctttccaat tgctattatt cttactttcg accctttcaa ttagtaagct
     5881 aaggactaac tttacatctc caatcaaaga tacattagat tctatatttt tacttatttc
     5941 agatgggtct atatctatgt gtattatttt tgcattttta gcaaattcag agcttttact
     6001 tattactcta tcactaaatc tagcacctat cgctatcact aaatctgaat tagaaagggc
     6061 taaattactt tctctacttc catgcattcc aaccattcca agagatagtt cattttttct
     6121 atctatatta ccaagaccca taagtgtatt tagtacaggt gtatctatct tagttgcaaa
     6181 tttttcaaga atatcctcgc tatctgaaga cttaactcct ccaccagcat atattacagg
     6241 ctttttagat tcttttatga tatctattgc ttcatttaaa agcttgacat taatttcatc
     6301 atctaaacta aaatcactct tataatctat ataatcatct atttgacata aatcataatc
     6361 ttcatcacta aaatccattt ctgctaaaaa taaatccttt ggaacatcta caagtacagg
     6421 tccttttcta cctgaatttg caactttaaa agcttctttt attgtaggaa ctaattcttt
     6481 agtgtttcta actaaataat tatgttttgt catagataat gttgctccag ttatgtcaat
     6541 ttcttgaaaa gaatctttac ctaataagct tgttggaacc tgacctgaga ttacaaccat
     6601 aggtgaagaa tccataaaag ctgttgctat tccagttatg gcatttgttg ctcctggacc
     6661 agacgttgta aaacaaactc ctactgtatt agtacttctt gcgtacccat ctgcggcatg
     6721 aactaaacct tgttcatgag atgtccttat atgtttaaaa tcatctgaat aatcgtatag
     6781 agcatcataa agaggtatta ctgctccacc aggatatcca aatattgtgt caatgccctc
     6841 tttctttaaa cattctaaaa ttacctttgc tccattcata cgcactacta tacaccactc
     6901 tttccctcaa actaatccat ttaatatata tttattttaa gaatgacatc atacttctta
     6961 gatttctacc aacttcggca atttctgtat tatattcttc ttctctagtt tttaggaagt
     7021 tctcacgtcc ttcttcattc tctttaatcc acgcttttgc aaattttcca ttttgtatat
     7081 cctctaaaac attttgcata ccttgttttg cggcatcagt tattaccctt ttaccagata
     7141 cataatctcc atattctgct gtgtcactta tgctatatct cattctttca aatccacctt
     7201 catatataag gtcgactatt agtttcattt catgtaaaca ttcaaagtat gctacttctt
     7261 tttgataacc tgcatctaca agcgttttat atcccaattt tataagttca cttattccac
     7321 cacaaagtac tgattgttct ccaaataaat ctgtttctgt ttcttcttta aaagtagttt
     7381 ctaaaacacc tgctcttgtt gctcctatcc cctttgcata tgctaaaact gtctctgtag
     7441 cttttttagt gtagtcttga tatactgcaa atagtgcagg aactccactt ccctttgtaa
     7501 atacatttct aactaagtga ccaggtcctt taggagctac cattacaaca tcaacaaatt
     7561 ctggtggttg tacttggtta taatgtatat tgaatccatg agcaaaagct aatgtctgac
     7621 cttcttttaa gttatccttt acactctctt cataaacttt cttttgtttt tcatcaggca
     7681 ttaacatcat tatcaaatca ctttcttttg tagcttctgc tacactctta acttcaaatc
     7741 catcttcttt tgctttttgt gctgatttac ttccgtcata aagacctatc actacatata
     7801 caccattatc tctaagattt tgtgcatgtg catgtccttg gcttccataa cctaaaactg
     7861 caaccttttt attttttaaa acctctaaat ccacatcttt ttcataatac attcttgcca
     7921 ttttcatttc ctcccaatac tttttcttat ttttatgata tttattttta ttagaaattc
     7981 atactcatta tcattatttt aatattattc aacagattta aattctaaat ttatatagtt
     8041 ttacaattat atttttagaa ttttcagttt ttatcagata tgataaactg acaaattcaa
     8101 tgatacttgt actttaatct aagatttttc ttatttaata tttatcttta gttttttatt
     8161 ttaaaataag catatttatc tttagcttta taaaaatata tttaattaat gtttcattct
     8221 ttcaacagtt atctctctta tatcatgtag ttttttcatg tggtttaaga cagaatctaa
     8281 agatgtcttt tcttcattta ctattaattt tatacctgag ttattcatat tatctacagt
     8341 catgcttatt gattgtatat caacttctct tcttcttaga gtcatagcca ctcttaaaaa
     8401 agaatctatt ccttggttta atttagcata tacagtattt tccataaaaa ttcctccttg
     8461 tttttattat ttttttatta attttatatg gattaaattt atttatatca cttaaaataa
     8521 aaaaactttc atctttataa gcaatataaa tattacttat aaggacgaaa gttttattcc
     8581 gcggtaccac cttatttgta gatataaaaa tacatctacc cctctttttt caggtcggat
     8641 aattaattac cttaacctta gtcatgtaac gttgacagac gtttgtactt actaaaaata
     8701 ttcagtacaa aagctccaga gtgatcatta tactaccgtt aacaccaatt ctcagcaaca
     8761 ttggttctct gtggataaca ctattagtat tgttgtctct gtcattgctt ttaatttttt
     8821 aattttatat ttcagttctg ttttgttgtt aatatagatt atattcccta tcatttacaa
     8881 tgtcaacgac tttttttaaa attttcagat tatttatttt tttcacaata atacttctct
     8941 tacattttac acctatgttt tggaataacc gttttcagat tgttatttta tatccattta
     9001 tttttaacca attttctttg tctttataat ctggcataat agattttact aaatcataga
     9061 attttttaga gtgattcata tgtactaaat ggctaaactc atgtattatt acataatcaa
     9121 ttacatctaa ttttgccata gatatttttg agtttatata aatatttttt tgagatgtgc
     9181 aacttcccca tctctttttt tgttctttta ccttaacact tgctggaact aattgattca
     9241 ttatttcata atttaattta agaaaatcta ttctctctaa cactaatctt tcactttcta
     9301 tcttatacca agactttaaa gattttttaa taaaatcagt acttgtatca tttgtctgaa
     9361 cgattatttc atattcgtta atagttattt tacagttact ttttttaaca gaatctttca
     9421 acatttcttt tataactaga tggtactctt cacctaaata aagaaatttt tcgccagtta
     9481 taaacattct ttgttcgtat acttcttctc ttcctttata cttttctgac tttttaagta
     9541 tccagtctcc tttttcaatg attatatctt ttattatttt tttagacatt ttaggagggc
     9601 tcatcacaat tacttttcca aatggatcta tttttataga aatatttttt ctatttttat
     9661 atactaaaat tacttttata tcaatccctc taaaattaac atttaaaata tcactatctt
     9721 tgcttcctat gttcattagt ttatttttcc ttattagctc tacagtttat aagctcagca
     9781 gctatacata cagctatttc agctggtgtt tgagcattca attttatgcc tattggtgca
     9841 tgtactcttg ataattcttc cttcttaaaa ccttttttca tgagtttttc gtacgtcaat
     9901 ccaatttttg ttctacttcc aatcatacct atataaccag catctctttt aagagcccat
     9961 tctaaagcac ttgaatcata taaatgacct cttgttaaaa taattatata gctattttca
    10021 tcagttggaa aatctggtat ctcttcaaaa gaatctaaaa ctattacttc tgattctggg
    10081 aacctttcat gattagcaaa ttcttctcta tcatctatta ctactgttct aaattctaaa
    10141 agagatagta tcacagcaac atctcttgaa atatgacctg ctccaaatat atacgcttta
    10201 ctcgtatttt cctttagtct cttattgaat ttttctatat tactttttaa tttactatct
    10261 ataaaatcta tttgtatttc tccagtaccc ccacatatca ttcctagttt tgccacatca
    10321 gatttgttta gagtaaattt atacagcatc gattctctat tttttataac attaattgca
    10381 tgttttatac attctgcttc tacactacct cctcccactg aaccaaatat agtagaatca
    10441 ttttttacaa tcatcattgc tccagtttca cgtggtgttg aacctgaatg agaagttatt
    10501 gtagcaaatg caaaatcctc attattttct attaatctta ttgcttgttc atatatattc
    10561 atataattct ccttaccagt ctatctaaaa atcttctata tatctatctt aggcttttta
    10621 ttacttcatt tggtatcaca aattctactg ctctcatata accaggcgct aattcatatt
    10681 taccaaaact aattacaaga tttccatttc tattaaaata aaaatcttga cacttattta
    10741 tacattcaaa ctctttcact agggaacctt tatcttggtc tataaaataa gattttgtat
    10801 tatctttctt aatgtaattt ctcatctgtt cttttatatt atcacttata actctaatat
    10861 atctactatc tttaaaaaat attggtaaat taatagtatt tctctatttt tattgatagt
    10921 gtaatgcttc ttagttacag atgaggaggc ttctatctat tcttttgtca tttctattga
    10981 taaaaagtcc tcagaattat tcttgacatt atagcttaca tttaggtatc tatacccctt
    11041 attatttttt tattttattt tgaagttcac tgtaaatttt tatcccatat tataaaaatt
    11101 cattatttaa tttaatctct aaatttttat cttttaaacc ttttatttta ggaatactaa
    11161 ccttcacttc ataattgtct tgtttaaagt tgaaactctt agcatgtaac acctttatca
    11221 tatcactagt ttaagtctca ttagaaaaat tatctgaaaa tatagggttt gtaattacta
    11281 atgatactaa taaacatatt gaaactatag caattttaaa tatagatttt ttaagttttt
    11341 tatatgtagt tatattgcac tccttaaaat atttatatat aaactactat taatttacaa
    11401 atcttttatt actttatatg gtattacaaa ctcaactgca cccatataac caggtgctac
    11461 ttcatattcg tcaaaacaaa ttacaagttc ttcattttta ttaaaataaa aatcctgata
    11521 tttattaatt gttttaaaat cttcaacagg taattctttt ttttgatcta taaaatagaa
    11581 ttttgtacta tcccttttca tttgttctct catttgttct tttatattat cactgataac
    11641 ttttatatac ttatcatctt taaaaagcat tggcaaagtc aagactattt gtcttttttt
    11701 atcaatagta taatgtacct tactcacata tgatgaagcc tctatctcat tttttgtcat
    11761 ctctattgat agaaattttt tagaattatt ttttacacta taactcaaac ttaaagattt
    11821 atgtccctta ttctttgatg attgtatttt ctccattctc tcttcaaatt gatgataaag
    11881 tctttttcca tcttccataa aactactatt taatctttgt tctaagtttt tatcttttaa
    11941 acctcgtatt ttaggaatat caatctcagc ttcataattt tcacttttta aactataatt
    12001 ttttatagtt atcacttcta ctattgctcc aattacaggt attttagaaa aattgtctgc
    12061 aaatacagga cttatattta ctaatactgt aaatatacat gcaaaggcta ttgcaacttt
    12121 aaatcttaat ctatttactt ttttattttt ctcttgatat atattttcta ttttttcatg
    12181 atttattata gtatctagtt tcttgggtat tggaatttta tgatactcct cttttaactt
    12241 atttaacctt tctttgcttg tcattctttt tatccctact cttcaatttt taattttagt
    12301 ttttttaaag cagtatataa cctagtttta acagtattca cattttcatc caatattatt
    12361 gctacttctt caatctttaa atcttcaaaa aatcttaata ttataataga tttgtattca
    12421 ataggtaatt cttctaaagc tcttcttaaa tccaaatcag tatatttatc atactctcca
    12481 gtctcatcta caaggtcaat atctgtcata ttattatatt tacggttttt tctaataaaa
    12541 tctatactcg ttctaattaa aattttataa aaccatgtct ttatataatt tacttcttta
    12601 atattttcca ctgagtatag tgccttatac atagcttctt gaaccacatc taaggcatct
    12661 tcctcattct tcgtataact atatgctatt ctataaaaag agtctttatt atcgataatg
    12721 tatttctcaa ctttcgttct ctttaaactc ttaaacattc ctgtaaaatc tccctccgat
    12781 tacaggccag gattgagctg ttagaaattc tatctgtcta ttttagcatc ttcatttaat
    12841 ttttttatat gctcaatata cttttcattt aaaatagatt ctcttattaa actctgacac
    12901 tcattaaaac ttttttgtct ttctctttta tcagtaactt tgactatatg atatccataa
    12961 ctagtttcaa acacctttga aacttcattt ttctttaatt tgaacacttc ctttgtaaac
    13021 tcagcatttt tgtcatcctt tgaaaaatat cctagctgtc ctccattttt tccagttgct
    13081 ttatcatctg aatatttttt agccaaactt tcaaaacttt ctccattttt gattttagtc
    13141 aatatattat ctgccttttt cttcaaagct tcttttttat ctttacttac ttctttttta
    13201 tttttgtcta aagtcgatat aagtatctga gaagcactta tttcttctac attaaaatct
    13261 tttttatgac ttgtatagta ggcttccata tcattatcac ttatgtttat tctatcttca
    13321 aaggcttttt tatttttatc tatggctaaa tctcttgata tttcttgttt taaatattct
    13381 ttatcaacac ctgctttgtc cattttttcc ttatagcttg tattcaaatt catcttatct
    13441 tctagttctt gatattttga gtctacttca cttttacttg gagttagtcc tttatcctta
    13501 gcctcttgat ataaaagttc attatccacc ataaacgata ttataacatt ttctaattta
    13561 tttctacctt ttttatccaa agttttttct aattcatcta aagactgccc atttatataa
    13621 ttattagtag cagataagac tgcttttgtt tttttgtatt gttcctttgt aatttccaca
    13681 tcatttactt ttgctagact tttattacaa ccaaccatca tcaaagacaa tattccgacc
    13741 attcctatat aaagtttttt attcaatata cctacacctc actcattttt attctcaatt
    13801 tctaaaaata tcaattaata ttcttatatt ttaactactc taaattacgt aaccaataat
    13861 tttttggtat ttgattttga tctactggag caaattcata accttcatct gataaaaatt
    13921 ttaaaactga atctaaagct tctgcactct gttttttctc atgcattaaa actacaagtt
    13981 ggtctttatc ttcacctttt ctattcttaa tatgattttt tacattttgt ataatttgag
    14041 aagaattaga tctccagtct tctgtatcta agtcccagtc ccaaatttta tatccagcat
    14101 caactaaagc ctgataagaa gcttgtggtg tatatggttt gcttccataa ggtattctta
    14161 ttacttttga atgtttgcca gttatcttat aaaaagtttg atcatttgta tcaaattctt
    14221 cttttgctga tgtactagta acataaagct tgtgaatatc atgtgataca ctatgaaaac
    14281 ctgctgtatt cccattttta attatatttt taacttgctg tggatattcc ttcatatttc
    14341 catctatcat aaaaaaagtt gctttaacat tatatttatt taaagttttt ataatctggt
    14401 cagtaaattt agatggacca tcatctatag ttatgtatgc tacttttcca gaacttttac
    14461 ttggcccttt actaacttct gttgctgtat tagatactat atctgtaata gtagaacttg
    14521 acagttttgc atttctacta atttcatttt ctttcatatt taaagagcta acgcgtgcac
    14581 caactactac aaaagccaga cacactacaa tagcacttaa aagcaaatac aacttctttc
    14641 tatttatttt tttcttttta tttctcatcg ttcgacctcc aatattacta tatttttaca
    14701 tttatatttt tgtagtattt tattccaaaa atttacacta catatatata gacgttgaac
    14761 ttcgacaaaa agttttgctt aatttttaaa atttgttatt ttgatgatta ttatgctgta
    14821 taatttatgt ataattgtat agaatgttga aaattgagta ttatatagtg ttttttacaa
    14881 ttaattctaa ttaaaatgtt atttataatt tttaaaattt atgattcttt ttttataaat
    14941 tagaaatatt aaacaaagaa taaatactaa aacaactatt tgccacattt ttaatacata
    15001 tattatatat tcatattgtt taatttttgt tatcaagtaa aattttctaa tcttcctcat
    15061 aaat
//
LOCUS       DAFNNB010000092        15055 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0093, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000092 DAFNNB010000000
VERSION     DAFNNB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15055
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0093"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            298..2379
                     /locus_tag="L3M60_002361"
     CDS             298..2379
                     /locus_tag="L3M60_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HBN6227017.1"
                     /translation="MDKEIVSILQILLNSTLITTNGLQEESNSSKRQITYRINKINDM
                     LKVKNVPLICLRADKDIIVRKETKKAIKEILEKNYSKNTYYFSKAERLLYMYLMLFIN
                     MEDISINHFINSIKVSRSTVNLDFKDLIPNLEKKGIEVKNNRINGYYLVGNEMEIRRV
                     LIKSIIETLSNEKSSRVFDIFIKEYNLDSFEEDKEIILKLIKKYEITFVEDRLIEFIY
                     IFIFLKARMYSTRIVTHENVDIPNIAVMSSMKEYKFAKELLETYESEDKIKSHNIMYI
                     SAWILGISVGNYEEDTEDKDVISKIVNKMMTKFGYLSGVYYISQEKIFKQLYSHFRPA
                     YYRLLFKLPIYNPICEKVKEEYKLVYRIVSNAMKEFCGLFGEEIQEEELAYLTMHFAT
                     LFSNKKEFDGFRKRIALIVCSNGVGSSAILHAELTNLFPDLHFIPMSESVGLENMSEP
                     VDIVFTTNYNTEGLKTDVPVIKVSPVMTTKEKYKITREVYIQLGDIFLRQPKIDEVMN
                     IIKKYANVTSESILSSELLTYFTQIENFTSKEGEGPMLSEITNETLVKIKVKAKNWED
                     AIRQSASVLVENNKTTEGYVEAMINSAKASGPYIVITKHVALPHARPEAGSKEIAIGI
                     ATLDSPIEFGNDENDPVKYVFCLSAIDNNSHLRAMSELVELLEEEEFFKVLDSAKEPK
                     EIIDYIKTHEL"
     gene            2394..2681
                     /locus_tag="L3M60_002362"
     CDS             2394..2681
                     /locus_tag="L3M60_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6227018.1"
                     /translation="MYKILIACRAGVGSSLMLKIKTQQIVKENNFPIEVEHGSLDSLN
                     GFNGDAVETLIDVAEELKNKHLKFDIIGIHNIVDRNEIKTALEKFLASKEG"
     gene            2708..3979
                     /locus_tag="L3M60_002363"
     CDS             2708..3979
                     /locus_tag="L3M60_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HBN6227019.1"
                     /translation="MEFIISLLSTPAVLLGVVACIGLILQKKSAVEVFTGSSKTLIGF
                     LIFGIGASAMTGALQNFNKLFQHGFGITGVIASPEAATALAQGTYGFAVSCTLILGFI
                     LNLVFARITKFKNIFFTTGHSLFFSCVLVLIMKAHGFDNTITILIGGTILGFMSAALP
                     QFCQPFMRELTGGDEQAIGHFNMIGYGLSGYIGRLFSKHKDDTTETIEFPKWLSIFRD
                     FLMGLSIVMLILFYIATLKAGQAFTQEIAGSTHWLVFPLIEAFTFVAGMSILMSGVRM
                     FLAEITAAFVIISEKYIPGSRPALDVPTVFPYAPNAVIIGFITAYAAGLLAIAIMASF
                     PSIFPVVIIPAAHICFFSGGTAAIFGNSSGGWRGAIAGSFVVGLLLAFLPVILYPVYG
                     TLGIEGATFPNIDYNVVGSILHNILQFIKPI"
     gene            4036..4635
                     /locus_tag="L3M60_002364"
     CDS             4036..4635
                     /locus_tag="L3M60_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBN6227020.1"
                     /translation="MENTLSEFLDNISNELSEFIASIDETSINKACNLIFEAEKNHGR
                     VHVTGIGKPGHVSGYISSLLSSTGTPAYILHGTEAVHGSSGQVVEGDVVIAISNSGET
                     QELKGTLETLKINGAKIIGVSGNESSFLKNISDIFLFAGVKQEGDCLNKAPRASILAE
                     TIVLQSLSVALQYAKGLNTQQYLKWHPAGSLGKSIREGI"
     gene            4640..5524
                     /gene="dapA"
                     /locus_tag="L3M60_002365"
     CDS             4640..5524
                     /gene="dapA"
                     /locus_tag="L3M60_002365"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBN6227021.1"
                     /translation="MKYKGIICAMITPFDENQNINSRATCQLIDYLIKNGIYGLFILG
                     TNGECHVLTDDEKVEFAKIVINHTNNRVPVFVGTGGNSTREVIDLSKRMEAVGASALS
                     IITPYFVTPTQQELILHYETIANSVNLPIIMYNMPDKTGINIEPESVCKLSKIKNIVG
                     IKDSSGKLDNMKAYIEVTKNEDFSVFSGSDSLILDSLKAGSQGAVAATANFLTKIDIS
                     IYENFMRGDLEEAQKAQSSIEEFRRILKLGTIPSVLKKTIVLNGIDVGTARLPVIEPT
                     GDALEEIKNVVENYREMF"
     gene            5653..6285
                     /locus_tag="L3M60_002366"
     CDS             5653..6285
                     /locus_tag="L3M60_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketohydroxyglutarate aldolase"
                     /protein_id="HBN6227022.1"
                     /translation="MKKMNITKRMAECGALAIVREEKLNRACEIVEGCIKGGITVVEM
                     SYTLNNAGEIIQGLNKKYGEALCVGAGTVLDSETARHAILHGAQFVISPNYNEDVAKI
                     CNRYQIPYAPGCTSLTESVEALSLGAAFIKAFPISDFYGPKLVKVFKTPIPDMPVLAS
                     GGINLENLHVWLENGVDVCGFGGLLTKGTVEDIAKNAKKIREIIKNIREV"
     gene            complement(6471..7748)
                     /locus_tag="L3M60_002367"
     CDS             complement(6471..7748)
                     /locus_tag="L3M60_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6227023.1"
                     /translation="MENTVKNSKIFYGWWILLACIIVMALGYAPIVSLASLFIKPITE
                     DLGFTRSAYTLSTAIISLMCMFMASITGKLMSTKHMHLIHVICVAGLSIFYGLYSLAT
                     SIFHFYVVSFFIGLFTYGATKMPVSILISNWFYEKRGLALSLSMAGTSISGTLLSPLV
                     VKLITNYGWRQTYVYLAIVMFLIMVPATIFIVRQFPSCKGLQPYGYDRHQDNTNKNTI
                     KNEWNISLKEAKKLPVFWTFTFGVTLILLTGCILGHIPAYLEDSGYNPSLTASIVSTY
                     LFIAIWGKVVLGHVFDKLGSKAGVLLGNICFIISIVCLLFIEFKPMLYLMTFFFGIGT
                     CIGTVTTPVLTSRIFGTKHYPEIYGFVASCTCIGSIIGTPAIASIFDLTGSYKMAWII
                     LIILSIIMTLALVYSINEGHKYRLKDVSQQTIK"
     gene            complement(7750..8940)
                     /locus_tag="L3M60_002368"
     CDS             complement(7750..8940)
                     /locus_tag="L3M60_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6227024.1"
                     /translation="MSDMFMNLAKQFESEIISWRKHFHENPEIGMELPNTVNFIQKKL
                     EEFNIEYKVGCNGYSITGLIGNKNSGKCILIRSDMDALPVIEQTELEFKSNNNYMHAC
                     GHDAHMAILLGAAKILKLKENELEGSVKLMFQPGEEVFEGAKAMISEGVLENPKVDSA
                     FGMHVVPTMPNKSVVYGKEFMSSCYGFKITIKGKGGHGSQPENCIDPINTGVHIYLAL
                     QELISRECPPQELATLTLGQFKSGEACNVIPNEAVLQGTLRTFSPTTRDLLINRINEV
                     VQSVSITYRCSADIEVLSNVPALICNDELNKKFMSYIYELDKNIKMSEFNHTMGSEDF
                     AYISEKVPTCFMYLGAGIEDTSKWYVAHNPKVMFNDKCLALGSAIYAQCAYQWLKNMS
                     KESL"
     gene            9125..10003
                     /locus_tag="L3M60_002369"
     CDS             9125..10003
                     /locus_tag="L3M60_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6227025.1"
                     /translation="MNLNYFQYLVEVANIGSISKAAEKNFVSQPYLSGIIKKMEDEVG
                     FLIFKRTNLGIELTEKGEIFINEAKKILIGIENIKNINTKNEEGLSIGMVYSLFFTKC
                     FLKFKKFKPTDLQDKVYEMSPMEIFKALIDNKIRLGLFSYATKKKDKYWNIAKKHHLK
                     CWSIYEDLHPYVIMSRSHPLALNDSITISDLNSNPYVVYDKSEFLIYLDMIGIRKHPD
                     LLLVSDRGSFSDAILDNNYLGITTYFSEINEQDNKFIYIKLEDCDETIGIGYAILDNY
                     KLSQREKDFIKFIKNK"
     gene            10782..13289
                     /locus_tag="L3M60_002370"
     CDS             10782..13289
                     /locus_tag="L3M60_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6227026.1"
                     /translation="MKEDIGLHRMCKEEQVTKNEVISEDILKSMSMGVSKHFLDDSFT
                     ILWGNTSFYQLFGYTEEEFLFKFSSLKDYYEQNLYNFESMKNHFLEAYHRGEKSTEYQ
                     ICISANNVDPVWVIMNGILIESEENEKSMVYILYSRINELEIKQEEASKMEKESTENF
                     KWMMSEYAGNVYISDMDTYELLYLNKQSCNILQASVNELIGRKCYEVIQDRTSPCPFC
                     TNPYLKKNDVYEWEFYNKKLKRTFMIKNRMLNWKGRRARIELSYDMYSAEYKLAKKDQ
                     ERESILKTIPAGMVRVDARDYLTILWYNDIFLNMIEYTKEQFAEELDNQCGYLFSEDY
                     KRSKKLAQDLKESGDNVVFEARIYTRSREERIWTVTLCYVSGEDSWDGIPSFYSIGID
                     ITKERKQIEKLQYISEKDALTGIYNRAETERQIKKYFEENLNSMGALFMIDTDNFKQI
                     NDTEGHMIGDIVLTEMASGMKKIMCDSDVVGRIGGDEFTIFMKDISSVKDAEKKAEEL
                     LYMFRHLFQKEKSSLKVTCSIGIAIYPKDGTTFKEIYTRADKALYQAKNIGKNNYVIY
                     NQDSFKELEAFNCSSLGTVIDSEKQYAECPDNLTRYIFRMLYQTDNIDETINMILEVV
                     GKQFNVSRVYIFENTEDGRYTSNTYEWCNEGISSQMAYLQNNNYQDYEDYEKLFGDEL
                     VFYCRDIHTLSPKLEELFSSQGIHSTLQCAYKEDKVFSGFVGFDECTGLRLWSQEEIS
                     TLSLISQIISMFLQRKKNKKFNQQMQQYQNILDNIDQCICVINKENNFLLYVNKKFKN
                     TYSKLQIGQPCYLDAVDIHKISIAWNQKEAYLCISMI"
     gene            13632..>15055
                     /locus_tag="L3M60_002371"
     CDS             13632..>15055
                     /locus_tag="L3M60_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227027.1"
                     /translation="MKKIIYVLFTIELLIVSLLGLNIVKDNEINNILYNDTTSISVMF
                     KDYKKFNKNYSNWIKDIADENNVTISKYVFNNNEKLSIYTTDTSLNNNIKLKSGEFPN
                     TNTNSNEFISNINTNNSNQVGKFSTMSKDISILIRDFDKISEVGESGILYISTQNKDT
                     INKILKELNVDRSIFVARLHDRYVNSNLYLNPSIIRDLILIILCFLATIIHYSISVSR
                     EASILRLNGYSKLDIITRVIRSILRIMILSSITSLLIYIIYILKLNLGVRACLYFVIF
                     SIICILINIFISILIVGFNIRSSKYVLTLNGKKPYGFVNIIHFTLKIVFVSFLILSIN
                     NCILNYNILQTQLGNLSEWDKAKNVYNLTLKDTGEQSLGKEEVIKNAKIKGFYKSLVE
                     EKDAFLIDTTNFEKLENGSYMYEINSKGKKPEVSQYGKNIKINKNYLKVNPIYSNGKE
                     IFNLIDYDDKTINLLVPKKLQVHE"
BASE COUNT         5717 a         1779 c         2508 g         5051 t
ORIGIN      
        1 taatgtgctt tttatttata gtaatttagg cataattagt atcgttttga ttttttaaat
       61 aaatttttct tgagtttctt tacctaaaga aacctttttt atttataaaa tatataagtg
      121 taatatagta tctattttaa ataagtttaa tttttgtctc aatctaaaac aaaagttata
      181 cttaaatgaa attgaaaacg ataaaattct caaataaaat aagtattgtg gaggtgataa
      241 aaaaatccaa tgataatatg aagactaatc ttaataagaa agtagggatt aattatagtg
      301 gacaaagaaa ttgtatcaat tctccaaatt ttattaaata gcacattgat tacgactaat
      361 ggattacaag aggaatctaa ttcatcaaag agacaaatta cttatagaat aaataaaatc
      421 aatgacatgc ttaaagtaaa aaatgtacca cttatatgtt taagagctga taaagatatt
      481 atcgttagaa aagaaacaaa aaaagcaatc aaagaaattt tagaaaaaaa ctattctaag
      541 aatacttatt actttagtaa agctgaaaga cttttatata tgtatttaat gctttttatt
      601 aatatggagg atatatcaat taatcatttt ataaattcta taaaagtaag caggtctact
      661 gtaaatctag atttcaaaga tttaatacca aatcttgaaa aaaagggcat tgaggtcaag
      721 aacaatcgta taaatggtta ttacttagtt ggaaatgaaa tggaaataag gagagtgctt
      781 attaagagta ttatagaaac cttgtctaat gagaaaagtt caagagtatt tgatattttt
      841 attaaagaat ataatttaga cagttttgaa gaggataaag aaattatatt gaagttaata
      901 aaaaaatatg aaattacttt tgttgaagat agattaatag aatttatata tatatttatc
      961 tttctaaaag caaggatgta ttcaactaga atagttactc atgaaaatgt agatattcct
     1021 aatattgcag ttatgagttc aatgaaagag tataaatttg ctaaggaatt attagaaaca
     1081 tatgagagtg aagataagat aaagtctcat aatataatgt atattagtgc ttggatttta
     1141 ggtatatcag taggaaatta tgaagaagat acagaagata aggatgtaat ttcaaaaatt
     1201 gtaaataaaa tgatgacaaa gtttggatat ttaagtggag tttactatat aagtcaagaa
     1261 aaaatattta aacaattata ttctcatttt agaccagcat actatagact tttatttaaa
     1321 ttacctatct ataatccgat ttgtgaaaaa gtaaaagaag aatataaatt agtttataga
     1381 attgtaagta atgcaatgaa agagttttgc ggattatttg gagaagaaat tcaggaagaa
     1441 gagttagcat atcttacaat gcactttgca accctttttt caaataaaaa agaatttgat
     1501 ggttttagaa aaagaatagc attaatagtt tgttcaaatg gcgttggtag ttcagccatt
     1561 ttacatgcag aacttacaaa cttatttcct gatttacatt ttataccaat gtcggaatca
     1621 gttggattgg aaaatatgag tgagcctgta gatattgtat ttactactaa ttataacaca
     1681 gagggtttaa aaacagatgt gccagtaatt aaagtaagcc ctgttatgac tacaaaagaa
     1741 aaatataaga ttacacgtga agtgtatatt caattaggtg atattttttt aagacaacct
     1801 aaaatagatg aggttatgaa tataataaaa aaatatgcaa atgtaacttc tgaaagtatt
     1861 ctttcaagtg agttattaac ttattttaca cagatagaga attttacatc taaagaagga
     1921 gaggggccta tgctaagtga gattactaat gaaacactag taaaaataaa ggtaaaagct
     1981 aagaattggg aggatgctat tagacagtca gcttctgtat tagttgaaaa taacaaaaca
     2041 actgaagggt atgtagaggc tatgattaat tcagcaaaag catctggacc atacatagta
     2101 attacaaaac atgttgcact tccacatgct agaccagaag ctgggtcaaa agagattgca
     2161 ataggtattg ctactttaga tagtcctatt gagtttggga atgatgaaaa tgaccctgta
     2221 aaatatgtat tttgtctaag tgcaatagat aacaatagcc atctacgtgc tatgtctgaa
     2281 ctggttgagc tattggaaga agaagagttt tttaaggttt tagatagtgc taaagaaccc
     2341 aaagaaatta ttgattatat aaaaacacac gaattataaa ggagaattta attatgtata
     2401 aaattttaat tgcatgtaga gcaggagttg gctcaagttt aatgttaaaa ataaagactc
     2461 aacaaattgt taaggaaaat aactttccaa ttgaagtaga acatggttca ttagattcat
     2521 taaatggatt caatggagat gctgtagaaa cattaattga tgttgctgaa gaattaaaaa
     2581 ataaacattt gaagtttgat ataattggga ttcacaatat tgttgaccgt aacgaaataa
     2641 agacagcact agaaaaattc cttgcttcaa aagagggata aatttaggat aataaggagg
     2701 aatatttatg gaatttatta ttagtttgct gagtacacct gctgttttgc ttggtgtagt
     2761 tgcatgtata ggattgattc ttcaaaaaaa atcagctgtt gaagtattta cagggtcttc
     2821 aaaaacactt ataggatttt taatatttgg tattggtgcc tctgctatga caggagcact
     2881 tcaaaatttc aataaattgt tccaacatgg ctttggaatt actggtgtta tagcttcacc
     2941 agaagcagct acagcacttg cacaaggtac atatggtttt gcagtttcgt gtacattaat
     3001 tcttggattc atattaaatt tagtatttgc gcgtattaca aagtttaaga atattttctt
     3061 tacgacaggt cacagtttgt tcttttcttg tgtattagtt ttaatcatga aagcacatgg
     3121 attcgataat acaatcacta ttttaatagg tggaactatc ttaggattta tgtctgctgc
     3181 actacctcaa ttctgtcaac cctttatgcg tgaattaaca ggtggtgacg aacaagctat
     3241 tggtcacttt aacatgattg gttatggtct atcaggttat attgggcgtt tattttcgaa
     3301 acataaagat gatacaacag aaactataga atttcctaaa tggttatcaa tattcagaga
     3361 tttcttaatg ggtctatcca tagttatgtt aatcttattt tatattgcaa cattaaaagc
     3421 tggtcaagca tttactcaag aaattgctgg ctcaacgcat tggttagttt tcccacttat
     3481 agaggcattt acatttgtag caggtatgtc aatattgatg tcaggtgttc gtatgttctt
     3541 agctgaaata actgcagcat ttgtaatcat ttcagaaaag tatattccag gttcacgtcc
     3601 tgcactagat gttccaacag tatttccata tgcaccaaat gcagttatta ttggatttat
     3661 cacagcatat gctgcaggtc tattagcaat tgctattatg gcatcatttc ctagtatatt
     3721 tcctgtagta attattccag ctgctcatat atgttttttc tcaggaggta cagctgcaat
     3781 atttggtaat agttcaggag gatggagagg ggctattgct gggtcatttg ttgtggggtt
     3841 attacttgca tttttaccag ttatactata tccagtttac gggactcttg gtatagaggg
     3901 tgcaactttc cctaatattg attataacgt agttggttcg atattacata atattttaca
     3961 atttataaaa cctatttaaa ctaaattata taaagatata gtcatagtca ctatatttta
     4021 aggaggatac attttatgga aaatacatta tctgaatttt tagataatat atctaatgaa
     4081 ttgtcagaat ttattgcctc aatagatgag acatccataa ataaagcatg caatttaatt
     4141 tttgaagctg aaaaaaatca tggacgtgta catgtgactg gtatcggcaa accaggacat
     4201 gtatcaggat atatttcatc attgctttca tctactggaa caccagcata tatattacat
     4261 ggtacagaag cagtacatgg atcaagtgga caagtagttg aaggagatgt tgttattgca
     4321 atatcaaata gtggtgaaac tcaagagtta aaaggaactc ttgaaacact aaaaataaat
     4381 ggagcaaaga taattggtgt aagtggcaat gagagttcat ttttaaaaaa tatttctgat
     4441 atcttcttgt ttgcaggtgt taaacaggaa ggtgattgcc tcaataaagc tcctcgtgct
     4501 tcaattctag ctgaaacaat agtattacaa tcattgagtg ttgcactaca gtatgctaaa
     4561 ggtcttaata cacagcagta tttgaagtgg catccagcag gaagtcttgg aaaatctatt
     4621 agagagggaa tataatttta tgaaatataa aggaattata tgtgcaatga taacaccatt
     4681 tgatgaaaat caaaatatca attcacgagc aacgtgtcaa ctaattgatt atttgattaa
     4741 aaatggaatt tatgggttat tcattttagg cactaatggt gaatgtcatg tgttgacaga
     4801 tgatgaaaaa gttgaatttg caaaaattgt tattaatcat acaaataatc gtgtgcctgt
     4861 ttttgttgga acaggtggca attcaactcg tgaagttata gatttgtcta agagaatgga
     4921 agcggttgga gcaagtgcat tatctattat tacaccatac tttgttacac caacacaaca
     4981 ggaattaatc ttgcattacg aaactattgc taattcagtt aatttaccta ttataatgta
     5041 caacatgcca gacaagactg gaattaacat tgaaccagag tccgtgtgta aattgtcgaa
     5101 gataaagaat attgttggaa ttaaggatag tagtggtaaa cttgacaaca tgaaagctta
     5161 tattgaagta actaagaatg aggatttcag tgtattttca gggtcagatt cacttatttt
     5221 ggatagtcta aaagctggta gtcaaggtgc tgttgcagca acagcaaact tccttacaaa
     5281 gattgatatc tctatctatg aaaactttat gagaggggat ttggaagaag ctcaaaaagc
     5341 tcaaagtagt atagaagaat ttcgacgtat cttaaagctt gggacaattc catctgttct
     5401 gaagaaaact attgtattga atggaattga tgttggaact gcacgacttc ctgtaattga
     5461 accaactgga gacgcacttg aagaaattaa aaacgtagtt gaaaattata gagaaatgtt
     5521 ttaataaaag tagtgagaac agataacata actggcggaa gttacattag tatataatta
     5581 atatatgtat tataaaatat ggctaaaatt attggatagg taaagtggtt tgatgagtat
     5641 agaggtgata atatgaaaaa gatgaatata accaaaagaa tggcagaatg tggtgctctt
     5701 gcaattgtaa gagaagaaaa gcttaaccgt gcatgtgaaa ttgtggaagg ttgtataaaa
     5761 ggtggtataa cagttgtaga aatgagttat accctgaata atgctggaga aataattcaa
     5821 ggacttaata aaaagtatgg tgaagctctt tgtgtgggag caggaacagt tttagatagt
     5881 gaaacagctc gtcatgctat attacatgga gctcaatttg ttatttcacc aaattacaat
     5941 gaagatgtag cgaagatttg caatagatat cagattcctt atgctccagg atgtacatca
     6001 ttgactgagt ctgtagaggc actgagttta ggagcagctt ttataaaagc tttcccaata
     6061 tcagatttct atggacctaa actagtaaaa gtatttaaga caccaatacc tgatatgcca
     6121 gttttagcaa gtggaggaat taaccttgaa aacctacatg tatggttaga aaatggggtt
     6181 gacgtttgtg gatttggtgg tttattaact aaaggtacgg ttgaggatat tgctaaaaat
     6241 gctaagaaga ttcgtgaaat cattaaaaat ataagagaag tttaatattg ggtttgtatc
     6301 atagcagttt ataattgcta tgatacaaac cctttagtat ttgttatctt aatttaaaat
     6361 atagatgaaa ttagtaatgc aactatataa ggattgctaa tcaatatata ttttttatgt
     6421 atttatgaat ttgtaggtaa gtacaaaaga taaatatttt atttataaat ttactttatt
     6481 gtttgttgtg aaacatcttt tagcctatat ttatgacctt cattgatact ataaactaat
     6541 gctaaagtca taattataga cagaattatt aaaataatcc aagccatctt gtaagaacca
     6601 gttaagtcaa agatgcttgc aatagctggt gtacctataa ttgagcctat acaagtgcaa
     6661 gaggccacaa acccatatat ttctggataa tgttttgtac caaatattct acttgttaaa
     6721 acaggggtag ttactgtacc tatacaagtt ccaattccaa agaagaatgt catcaagtaa
     6781 agcattggtt taaattctat aaaaagcaaa caaactatag atattataaa acaaatattg
     6841 cctaataata ctcctgcctt agagcctaat ttatcaaata catgccccaa tacaacctta
     6901 ccccatattg ctataaataa gtacgtagat actattgagg ctgttaaaga agggttatat
     6961 ccactgtctt ctaaatatgc tgggatatga ccaagaatac atccagtaag caatattaaa
     7021 gtaacaccaa atgtgaaagt ccaaaatact ggtaactttt ttgcttcttt aagagagata
     7081 ttccattcat tttttatagt atttttgtta gtattatctt gatgtctatc atatccataa
     7141 ggttgcaaac ctttgcaaga aggaaactga cgaacaataa agattgtagc tggaaccata
     7201 attaaaaaca taacaatagc aagataaaca taagtttgtc tccatccata attagtaatc
     7261 aacttaacta ctagagggct aagtaatgtt ccactaatag aagtaccagc cattgaaaga
     7321 cttagagcaa gacctctttt ttcataaaac cagttagaga ttaatatgga tacaggcatt
     7381 ttagttgcac cataagtaaa aagaccaata aagaatgata ctacataaaa gtggaaaatt
     7441 gatgttgcta atgaatataa accgtaaaat atagaaagcc ctgcaacaca gataacatga
     7501 attaaatgca tatgtttagt agacataagt tttcctgtga tagatgccat aaacatacac
     7561 attagtgaaa ttatagcagt agatagagtg taagcacttc tagtaaaacc aaggtcttca
     7621 gttatgggtt ttataaataa acttgctaag ctaactatag gagcatatcc taaagccatt
     7681 actataatac atgctaagag aatccaccat ccataaaata ttttactatt tttaactgtg
     7741 ttttccatat tataaactct ccttactcat attttttagc cattgatatg cacattgagc
     7801 atatatagct gaaccgagag caagacattt gtcgttgaac attacttttg gattatgggc
     7861 aacataccat ttagaagtat cttctatacc agcacctaag tacataaaac atgtaggaac
     7921 cttttcagaa atataagcaa aatcttcaga acccatagtg tgattaaatt ctgacatttt
     7981 aatgtttttg tctaattcat agatataaga catgaatttt ttatttaatt catcattaca
     8041 tattaaagca ggtacatttg ataaaacttc aatatcagca ctacagcgat aagttataga
     8101 gacactttgt acaacttcat taattcgatt tattaataaa tcccttgtcg ttggagaaaa
     8161 tgtacgaagt gtaccctgta aaactgcctc attggggatg acattacaag cctcgcctga
     8221 tttaaactga cccaaagtta gagttgctag ttcttgaggt ggacattctc tactaatgag
     8281 ctcttgaaga gctaaatata tgtgaacgcc tgtattgata gggtctatac aattttctgg
     8341 ttgagaccca tgaccacctt ttcctttaat ggtaatctta aatccataac aagaagacat
     8401 aaattctttg ccataaacca cagacttatt tggcatagta ggcactacat gcattccaaa
     8461 tgctgaatct acttttggat tctcaagaac tccttctgaa atcatagcct ttgctccttc
     8521 aaaaacttct tctcctggtt gaaacattaa ttttactgac ccctcaagtt cattttcttt
     8581 aagctttaga atcttagcag ctcctagaag tattgccata tgagcatcat gtccgcatgc
     8641 atgcatataa ttattatttg atttaaactc cagctctgtt tgctctatta ctggaagcgc
     8701 atccatatca ctacgaatta atatacattt tccgctattc ttattaccta ttaaacctgt
     8761 tattgaatag ccattacaac caactttata ttcaatgtta aattcttcta attttttttg
     8821 aataaagttg actgtattgg gaagttccat acctatttct ggattttcgt gaaaatgttt
     8881 tctccagcta ataatctcac tttcaaactg ttttgctaaa ttcataaaca tatctgacat
     8941 atttatctct ccttttaaag attttaagtt ttaattatac ttatccaaaa acttgtaatt
     9001 aatactaagt tgtgttagtt ttatgatagc atattaaatt tgtcaatcgg tattagcaaa
     9061 aaaacatgag gtataaggaa aaggttatat caatttataa tatatactag tttaggggga
     9121 tatgatgaat ttaaattatt ttcaatattt agttgaagtt gcaaatatag gctctattag
     9181 taaagccgca gaaaaaaatt ttgtatcaca accatattta agtggtatta tcaaaaaaat
     9241 ggaagatgaa gtagggtttt tgatttttaa aaggacaaat cttggtatag aattaactga
     9301 aaaaggcgag atttttataa atgaagcaaa aaaaatttta ataggaattg aaaatataaa
     9361 aaatataaat acaaagaatg aagaagggtt gtcaatcggt atggtctact ctttattttt
     9421 tacgaaatgt tttctaaaat ttaaaaagtt taaacctaca gacttacaag ataaagtcta
     9481 tgaaatgagt ccaatggaaa tattcaaagc attaatagat aataaaatta ggctagggct
     9541 attttcctat gcgactaaaa aaaaggacaa atattggaat attgctaaga agcatcatct
     9601 aaagtgttgg agtatatatg aagatttaca tccttatgta attatgtcaa gaagccatcc
     9661 tttagcatta aatgattcaa ttacaatatc agatttaaac agtaatcctt atgtagttta
     9721 tgataaaagc gaatttttaa tatatctaga tatgataggt ataagaaagc atccagattt
     9781 gcttttagtt tcagatagag gtagtttttc tgatgcaata ttagataata attatctagg
     9841 tataaccact tatttttcag agattaatga gcaagataac aaatttatat atataaagtt
     9901 agaagattgt gatgaaacta taggaatagg ttatgctata ttagataatt ataaattgag
     9961 tcaaagagaa aaagatttta ttaaatttat caaaaataag taaaatattc catatttaat
    10021 gtgggtagat tgtcatattt caaatcaata aataattagt atataccata taagtaattg
    10081 cttatatggt tatttttagt gaagcaatta ctttagaaaa tgaaacagat aattatgtaa
    10141 taaataagag atagaatttt tatttatcta acattaagta taaaagaaaa ttactatgac
    10201 ttaataaaat aagatattaa caatgaaatt cttgataaat accttttaat ttaggcatta
    10261 taaagtttaa ttgtacatgt gttacatatt ttcttgaaat ttataattaa tattaaactt
    10321 cctcagttca ataataatat actaaattta taagttagta ttattcatat gtaaagctga
    10381 gtgctttact aatttatatc gcataaaatt tcagcaattt tatcttaact tttctatata
    10441 aaaagaactg ctttaaaact tggggtatga tataatttca ttaaaagaag acggttttta
    10501 gtaaatacta gaaaataata tgtttttata gaaaaagtta aatatggatt taagtgaata
    10561 agaatcaata agtaaggtat taaattatga atagggtaca aaacattact gaattaaaag
    10621 ttttagaggg agaagtttag caaggatgtg tgaaaattaa ttctcaaaca ggaattattg
    10681 tatatgagat aaatttacac acattgaaga ttcactttta tttatactaa aaagtagatt
    10741 gtcttaaagg tgggtctaaa gataggagga taatcaaaat aatgaaagaa gacattggat
    10801 tacatcgaat gtgtaaagaa gaacaagtaa caaagaatga agttatttcg gaggatattt
    10861 taaaatccat gagtatggga gtcagtaaac attttttaga tgattcattt acaatactat
    10921 ggggaaatac atcattctat caattatttg gatatacaga agaagaattt ttatttaagt
    10981 tttctagttt aaaagactat tatgaacaga atctttataa ttttgaatct atgaaaaatc
    11041 attttttaga ggcttatcat cgtggagaaa agagtacaga atatcaaatt tgtatttcag
    11101 caaataatgt agaccctgtt tgggtaataa tgaatggtat attaattgaa tcagaagaaa
    11161 atgaaaaatc aatggtctat atattgtatt caagaattaa tgaattagaa ataaagcaag
    11221 aagaagcatc taagatggaa aaagagagta cagagaattt taaatggatg atgtcagagt
    11281 atgctggaaa tgtttatatt agtgatatgg atacatatga attactatac ttaaataagc
    11341 aatcatgtaa tatcttacag gcatcagtaa atgaactcat tggaagaaag tgttatgaag
    11401 taattcaaga tagaacttca ccatgtccat tctgtacaaa tccatatttg aaaaaaaatg
    11461 acgtatatga atgggaattt tataataaaa aactgaaacg aacgtttatg attaaaaatc
    11521 gtatgttgaa ttggaaaggg cgtagagctc gtatagaatt atcttatgat atgtatagtg
    11581 ctgaatataa attagcaaaa aaagatcaag aaagagaatc aattttgaaa accattccag
    11641 caggaatggt tcgagtagat gcaagagatt atcttactat actttggtat aatgatatat
    11701 ttttgaatat gattgaatat acaaaggaac agtttgcaga ggaacttgat aatcaatgtg
    11761 gatatttatt ttctgaagat tataaacgta gtaagaagct agcacaagat ttaaaggagt
    11821 ctggggataa tgttgtattt gaagcaagaa tatacacacg ttcaagggaa gaacgtatat
    11881 ggaccgttac attgtgttat gtcagtggag aagacagctg ggatggaata ccatcttttt
    11941 atagtatagg tattgatata acaaaggaaa gaaaacagat tgagaaatta cagtatatat
    12001 cagagaaaga tgcattaaca ggaatttata atcgtgctga gactgaaagg cagattaaga
    12061 agtattttga agaaaattta aattctatgg gtgcactgtt tatgattgat acagataatt
    12121 ttaaacagat taatgataca gaagggcata tgattgggga tatagtgctt actgagatgg
    12181 catcaggtat gaaaaaaata atgtgtgaca gtgatgttgt tggaagaata ggtggagatg
    12241 agtttacaat ttttatgaag gatatttcct cagttaaaga tgcagaaaag aaagcagaag
    12301 aattattgta tatgttccgt cacctatttc agaaagaaaa aagttctcta aaagtaactt
    12361 gcagtatagg aatagctata tatccaaaag atggtactac atttaaggaa atatatacaa
    12421 gagctgataa agctctgtat caagcaaaaa acataggaaa aaataattat gtaatctaca
    12481 atcaagattc ttttaaagaa ttagaggcat ttaattgttc ttctcttggt acagttattg
    12541 attcagaaaa gcaatatgct gaatgtcctg ataatcttac acgttatata ttccgtatgc
    12601 tttatcagac agataatata gatgagacga ttaatatgat acttgaggtt gttggtaagc
    12661 agtttaatgt aagtcgtgtt tatatttttg aaaatacaga ggatggtcga tatacttcta
    12721 atacctatga atggtgtaat gaaggaatct cttctcaaat ggcttatttg caaaacaaca
    12781 attatcaaga ctatgaggat tatgaaaagc tttttggaga tgaattagtt ttttattgtc
    12841 gagatatcca cacattaagt cctaaactag aagaattgtt ttctagtcaa ggtatccact
    12901 ctacacttca atgtgcatat aaagaagata aagttttcag tggatttgtt ggatttgatg
    12961 aatgcacagg attacgtcta tggtcacaag aggaaataag tacattatca ttgatttctc
    13021 aaattatatc aatgtttttg caacgtaaaa aaaataaaaa atttaatcaa caaatgcaac
    13081 aatatcagaa tattttagat aatattgacc agtgcatttg tgtgattaac aaagaaaata
    13141 attttttatt gtatgtaaat aaaaaattta aaaatacata ttctaaatta caaattggac
    13201 aaccttgtta tcttgatgct gtagatattc ataagatatc cattgcatgg aatcaaaaag
    13261 aagcatactt atgtattagc atgatttagt ggtaaggtaa agtttgtatt gaacttaaac
    13321 taaatttagt tttaaataaa atattatttt ggttgattac tgaaaatact agaatctatg
    13381 ttaatgaatt atactaattt tgtaccacta ttaaaatatt gtttgataaa aataaataaa
    13441 aatcttaaaa cagttggtta taaaaaatta actgtttttt attgttttta tatttgtgac
    13501 aaaatagaca catgccattt agaatgtgac aatatagtag ttgtttaaaa gagatgcaaa
    13561 gcattatact ttaattaaag tatagtgtat ttttgctttt aaaataaaaa caattagggg
    13621 gagagttata attgaaaaaa ataatatatg tattatttac aattgaacta ttaatagtat
    13681 ctttattagg tctaaatata gttaaagata atgaaattaa taatatttta tataatgata
    13741 caacaagtat atctgtaatg tttaaagatt ataaaaagtt caacaaaaat tatagtaatt
    13801 ggattaaaga tattgcagac gaaaataatg tgactatatc aaagtatgtt tttaataata
    13861 atgaaaaatt atctatatat acaactgata caagtttaaa taataatata aagttaaaat
    13921 caggagaatt tcctaatact aacactaatt caaatgaatt tattagtaat ataaacacta
    13981 ataatagtaa tcaagtaggt aaattctcaa ctatgagtaa agacatatca attttgatta
    14041 gggattttga taaaataagt gaagttggag aatcaggaat tttatatatt tcaactcaaa
    14101 ataaagatac aataaataaa atattaaaag agttaaatgt agacagaagt atttttgtag
    14161 caagactaca tgatagatat gttaattcaa atttatactt aaatccttca ataattagag
    14221 atttaatact aattatcctt tgcttcttgg ccacaataat acattattca attagtgtga
    14281 gtagagaggc ttctatttta agattaaatg gatattctaa gttagatata attactagag
    14341 ttataagaag tatacttaga attatgattt tatcatctat cacatcatta ttaatatata
    14401 taatctatat tttaaaatta aatttgggtg ttagagcatg tttatacttt gttatatttt
    14461 cgattatatg tattttgata aatattttta tatctatatt aatagtgggt tttaatatta
    14521 gaagttctaa atatgtgcta actctaaatg gaaaaaaacc ttatggtttt gtaaacataa
    14581 ttcatttcac attgaaaatt gtatttgtat catttcttat attatccata aataactgca
    14641 ttttaaatta taatattcta caaactcagt taggaaatct atctgagtgg gataaagcaa
    14701 aaaatgttta taaccttact cttaaagata caggtgaaca atctttagga aaggaagaag
    14761 taataaaaaa tgccaagatt aaaggatttt ataaaagttt agtagaagaa aaagatgctt
    14821 tcttaattga tacaactaat tttgaaaaac ttgaaaatgg aagttacatg tatgaaataa
    14881 attcaaaggg taaaaaacca gaagtaagtc aatatggtaa aaatataaag ataaataaaa
    14941 actatcttaa agttaatcct atttactcaa atggcaaaga aatatttaat ctaattgact
    15001 atgacgataa gactataaat ttattagttc ctaaaaagtt acaggtacat gaaaa
//
LOCUS       DAFNNB010000093        15014 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0094, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000093 DAFNNB010000000
VERSION     DAFNNB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 15014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15014
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0094"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            224..1492
                     /gene="eam"
                     /locus_tag="L3M60_002372"
     CDS             224..1492
                     /gene="eam"
                     /locus_tag="L3M60_002372"
                     /EC_number="5.4.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 2,3-aminomutase"
                     /protein_id="HBN6227028.1"
                     /translation="MNEQTRISLERAAELKSKIDDYIQARKTINRGLEKEEEINKRKQ
                     KILSILNGTEEDWNNYKWQLSNRITDVDTLSKIITLTKKEKEHIKEVGTQFRWAISPY
                     YLSLIDPEDICDPIKLLSIPTYIELEDEQEDLDPMGEEYTNPAGCITRRYPDRLIINV
                     TNECAMYCRHCQRRRNIGQQDSHKSKAIIQESIDYIRENEEIRDVLVTGGDALTLKDD
                     YLEWILSQLKEIPHVDYVRLGTRTLVTMPQRITDEFCNMLKKYHPIYINTHFNHPMEI
                     TKESKEACEKLANAGVPLGNQAVLLNGINNDKFVMRCLNQELLKIRVKPYYIFQSKHV
                     KGTKHFNTSVDDGLEIMEYLRGYTSGMAIPTYIVNAPKGGGKTPLLPQYLVSKGTDYV
                     MLRTWEGKVIKMEDEPAVDIKKLIKEQAQD"
     gene            complement(1585..1941)
                     /locus_tag="L3M60_002373"
     CDS             complement(1585..1941)
                     /locus_tag="L3M60_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227029.1"
                     /translation="MKKALISLLVLSCLLLTAPVNESSYADSQKNVPVSQNTKLTQEQ
                     AKELLVKYNNEVDYIYQGNASDFEALKAKNLNGYVFLPDADGDIGYFVNENTSEIYYF
                     HPSGYLELVNQQQNNK"
     gene            complement(2092..3186)
                     /locus_tag="L3M60_002374"
     CDS             complement(2092..3186)
                     /locus_tag="L3M60_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBN6227030.1"
                     /translation="MQDKKIYLNIVLTVVVSYILIKVIDNYKYFFGVISLLMSLLTPF
                     IIAFVLAYIFNPLVKFLESKLNFKRIYSLLLTYGVLIVILISFILFTVPSIVNSLADL
                     VAQIPTYIDKTEQFLFDLGKSLQSVDPNTLKEYGDKIMSVMPKFSNLLIGSLGGIFST
                     TFSVGKFIVQFLLGFIICFYILLEKEKFLLFTKKVVYISLGKKYGDFIIELCQSLNLN
                     IGKYFTGKILDSFIVGVLSGIGLYFLKSEYALLFGTIMGVMNMVPYFGPVIGMAPVVI
                     INLFSNPTTALTSLIYLIIIQQVEVTFIEPKIVGGQLGLSPFFTILAVTVGGGFFGIP
                     GMILSAPVMGVIKIYFCEFVNRRHDKIQME"
     gene            3646..4467
                     /locus_tag="L3M60_002375"
     CDS             3646..4467
                     /locus_tag="L3M60_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBN6227031.1"
                     /translation="MIKKEISNFRGSSDYVVSPELMASVNVAIALEKPLLIKGEPGTG
                     KTMLAQAISNELKKNLVIWNIKSTTKAQEGLYVYDTVQRLYDSQFGGEGVDDISKYIK
                     YGKLGEAFSSNQQVILLIDEIDKADLEFPNDLLWELDKMEFYINETKETVRAKQRPIV
                     IITSNAEKELPDAFLRRCIFHYIEFPDRDMMEEIVKVHFDKVEEHLLEQVMTTFYWIR
                     SLKDIQKKPSTSELIDWIQALTLSGIPIEKIEKEVPFAGILLKNNEDIESMQRHL"
     gene            4488..5684
                     /locus_tag="L3M60_002376"
     CDS             4488..5684
                     /locus_tag="L3M60_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBN6227032.1"
                     /translation="MFIEFFYLLRSRGVNVSLNEWMTLIEALDKGLCYSNFSNFYYLC
                     RMILIKSESDFDKFDTVFLEYFKGIEHQEEIPEEIMNWLKKPDIDLEEFERLEQNPNI
                     NLDKLRAKLEERIKEQDSPHNGGNYWIGTGGTSELGHSGKGQTGIRIGGNSTYGRAVL
                     EVAGERKYRDFRDDEVLNMRQFQVALKRLRQFSTRIDAPKTELDLDKTIEETCNNAGY
                     LKLFFEKPRKNTVKLLLLMDSGGSMRGYSTLCNTLFQSVSKSNHFKDVKIYYFHNCIY
                     DRLFTTPECWLSKSINTEWVLKNIDKNYKVIIVGDASMSPSELLHIGGNYRGPYNYTP
                     GIEWLKRFRRKYSKTVWMNPELRDGWDSINYWYQTQRMIQSEFNMFPLTVKGLEKALK
                     NLMVSR"
     gene            5850..9248
                     /locus_tag="L3M60_002377"
     CDS             5850..9248
                     /locus_tag="L3M60_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBN6227033.1"
                     /translation="MIIINYELIVKYNGDILRLEEELGVSVEILNSSYAIITSSNEED
                     VNTLLTYPEIEFIEKPFVLQTQDVQSFSSTGITGFKNRTGLTGKGTIIGIIDSGIDYT
                     LPVFRDSNGRSKILYYWDQSIQGNPPEGFREGTLYTNEDINNAIDGSMYIPISTTSLH
                     GTHVAGICATIASDARIIVVRVGNIQTDIFSRSTEFMRAIKFILDRALELKMPVTLNI
                     SYGSNEGSHRGTSLFEQYIDDMCLFWKNNIVVAAGNNADKGGHKRIRLRNNITEEVEF
                     IVGEGERILNVNIWPDFVDDFSVHLVNPSNNQTQTISLTSGEIRNTLGETRITGYFYP
                     IAPYSLTRRVTLQLSSNTQITPGLWRIIFEPINIVTGNVNIYLPTSEGLNRNTRFLIP
                     TQELTVTVPGTASRVITVGSFNSRTDIVSIFSGEGDTQLGVFKPDLLAPGEDIVSFLP
                     GGTSGALTGTSMATPHVTGVCSLFMEWGIVNGNDLFLYSQKLRALLLKVARRLSNQSY
                     PNNSSGFGFLNLSDVDLYTLSSINQDLETEDIGYRSINKSFMDEANNYIPIDGYNVQI
                     HNGLEKEKYISKNISRQSGILSGIDVVHTPEFEEELAGLGQSQNFLKISDSLGVLSID
                     NTDYNSIQQVLQLPSIIRTIPTTKMTLLGEINRGTFGGVVATEEIGVNFFKNNPNINI
                     TGRGTLISIADTGIDYLHRDFIYPDGTSKIVYLWDQTKEGNPPDGFYIGTEYTREDIN
                     RAIAANDPSLSQDEVGQGTMLSGICAGLGNVNSEYAGVAEDSELIVIKLGKIEGFYNS
                     AMLFAASQYAYNKAFELGRPLVINVSLGTSSLAGLTNRVNSEKAFFTRGLCVTAGAGN
                     EGNTQTHTSGIISHVGGSVEVELELNEEEEELSLELWLSRPDKADVIIVSPTGEESKT
                     VGVSNYNKVTGLFDLEGTEYSITYIYPTTFSGQQFTNVTLKNAKRGVWKIRLVGVYII
                     TGRYNLYLPNRKLLKSGTRFREVDPFYTINYPAIQDDLITIGAYNTINSSLWQSSSRG
                     PTIENRLKPDIVAPGVNIIAAYPGNTYATITGTAAASAHAAGAAAMYFQYTFVDGRYP
                     NQAYVQKIKTFMQAGARKDSNTVYPNTNSGYGLLDVRGMFDVLR"
     gene            9258..10931
                     /locus_tag="L3M60_002378"
     CDS             9258..10931
                     /locus_tag="L3M60_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBN6227034.1"
                     /translation="MEKSYCIIYQGDIESALQENGINRYMVLNSQLAVIYVPIDFDET
                     ILNNIIQVAWWEESAPMSSLIEITNNVDNGETITTAAGTEYIYENPYNDITGRGILLA
                     VIDSGIDYLHPDFINDDGTSKVLYLWDQEANTNPPPEGFIFGSEFTRSELNIAINRND
                     GSLSQDNIGTGTLVSGILAGNGRVNSKYRGITTESDLIVVKLKSYIGTYYAGRINYSV
                     SDFLAAITYVTNIARTENRPLIINLTIGVKSGAVATTSILDTFNILSSAGVVVVSGAG
                     NQGNTDIHYSGRFSSVGEVQDVIIQDGDDYALDITLNTNGPDKVGAQIISPSGEVSHD
                     IRYAPDFYIYRGKFNLENTTYAMRFIYPYITSGKENLEIRLRDIKPGVWTLRLTSELI
                     IGGEYDIYLPNKNLIAPDTRFLDPDSVATITMYAASDDVITVGTFNNKTDSMWIGSSK
                     GPIRGRGIKPDIVAPGVDIISTYKNGTYNTGTGTGVSSSIVTGVLALLMEYLEKQNNI
                     PRLSLFTQVLKTYLILGATKLEIYTYPNVSQGYGILNLKNTIQQIANTL"
     gene            11183..11377
                     /locus_tag="L3M60_002379"
     CDS             11183..11377
                     /locus_tag="L3M60_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HBN6227035.1"
                     /translation="MENKKARALKGKDNKRLKSSLLVGNESTAAWADVEKLKPHSKVS
                     IPSIKGVTEAKDWVDNGSKL"
     misc_binding    11535..11768
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            11845..13242
                     /gene="asnS"
                     /locus_tag="L3M60_002380"
     CDS             11845..13242
                     /gene="asnS"
                     /locus_tag="L3M60_002380"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBN6227036.1"
                     /translation="MQKEFITVKELYREKEQYIGKTVKVAGWIRTSRVSKNFGFIELN
                     DGSFFKNMQIVFDEKVENFKEVGKLAISSSILVEGELVSTEGAKQPVEIHAKSIVVEG
                     ESDSSYPLQKKRHTLEYLRTIAHLRPRSNTFSAVFRVRSIAAYAIHKFFQERNFVYAH
                     SPIITGSDCEGAGEMFRITTMDLNDIPKTEDGKIDYTQDFFGKEANLTVSGQLNAEIM
                     ALSFRNVYTFGPTFRAENSYTGRHASEFWMIEPEIVFADLEDNMELAEDMIKYVISYV
                     LENAPEEMEFFNSFIDKGLLERLNKIVNSEFVRITYTKAVELLIESGHDFEYPVEWGC
                     DLQTEHERYITEQIYNCPVFVTDYPKDIKAFYMRMNEDGKTVRAMDLLVPGVGEIVGG
                     SQREEREDVLLDRINEMGLREEDYWWYLELRKFGTATHSGFGLGFERIIMYMTGMSNI
                     RDVIPFPRTPKNAEF"
     gene            complement(13415..14590)
                     /locus_tag="L3M60_002381"
     CDS             complement(13415..14590)
                     /locus_tag="L3M60_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6227037.1"
                     /translation="MNLEKIKKFWGFPLDKKHKENVEKELCNYLYHTGKIICIIIVIS
                     QIIMMISISAREGGPFATPRRQWYFYMYVTLFCVSSVFMFVFSYLWNKKKDCCTFYIN
                     LWIIYVTFFCLWGCVITLLDQLGGNDLSVFSYIMLAAAAFSVLKLGESVIVFGGCFLF
                     LNFFLPGIQTIPGNEFSNIVNTFFITSLAVFVSTMLYRSRVFTYYDKLLISQQYEEIY
                     HINNLLNDKVMTDDLTKMNNRRYLEDILQKKLISHANQEPITGMMIDIDYFKQFNDNY
                     GHQKGDYCLQNIAKVILDFTSSENEHAIRYGGEEFFICIFNCDEEDAKLKSECLRKII
                     ETCNFERDDIPLKCVTVSIGVYTNKIERTITLNEFISNCDKALYTAKEQGRNCVAFY"
BASE COUNT         5874 a         1767 c         2566 g         4807 t
ORIGIN      
        1 catttgttaa aaaaaatgca taatatgttg taatttattg acgaaagtag aaaaatattc
       61 ataaatgtgc aaaataatag aaaaaatgca aaaataagtt caatttcatt tgttataaaa
      121 tctatatggc aaaatcaaat ttgcctatca gaatgatata aacacatcag aaaggaggag
      181 gctatcaaac aatataagta gttaactttg atagcaaaaa attatgaatg aacaaactag
      241 aatatcctta gagagagctg ctgaactaaa atcaaaaatt gatgattata ttcaggctag
      301 aaaaacgatt aacagaggtc ttgaaaaaga agaagagata aataaacgaa aacagaaaat
      361 attgagtata ttaaatggaa ctgaagagga ttggaacaac tacaaatggc aattatcaaa
      421 tagaataaca gatgtagata ctttatcaaa gattataact ctaactaaaa aagaaaaaga
      481 acatataaaa gaggttggta ctcaatttag atgggcaata tctccatatt atttaagcct
      541 tatagaccca gaagatatat gtgacccaat aaaattattg tctataccaa catatataga
      601 gttagaagat gagcaagaag atttggaccc aatgggagaa gagtatacaa atccagcagg
      661 atgtataact agaagatacc cagatagatt aataataaat gtaacaaatg agtgtgctat
      721 gtattgtaga cactgtcaaa gaagaagaaa tattggacaa caagattctc ataagtcaaa
      781 agctattatc caagaatcta tagattatat cagagaaaat gaagaaataa gagatgtact
      841 agtaactgga ggagatgctc ttacattaaa agatgattat ttagagtgga ttcttagcca
      901 acttaaagag ataccgcatg ttgattatgt tagattaggt actagaactc ttgttacaat
      961 gccacaaaga attacagatg aattttgcaa tatgctaaaa aaatatcatc ctatatatat
     1021 aaacactcat tttaatcatc caatggaaat aactaaggag tctaaagaag cttgtgaaaa
     1081 gttagcaaat gcaggagttc cattaggaaa tcaggcagta ttattaaatg gaataaataa
     1141 tgataaattt gtaatgagat gtttaaatca agaattacta aaaataagag taaaacctta
     1201 ttatatattc caaagtaaac atgtaaaggg aacaaaacat ttcaatacat cagtagatga
     1261 tggtcttgaa atcatggagt atttaagagg atatacatca ggaatggcta taccaacata
     1321 tatagtaaat gctccaaaag gaggaggaaa aactcctttg cttccacaat accttgtatc
     1381 aaaaggaaca gattatgtta tgcttagaac atgggaagga aaagttataa aaatggaaga
     1441 tgaacctgct gtagatataa agaaacttat aaaagaacaa gcacaggatt aaaacagtat
     1501 ttaaattcta taaaaagaaa caactttaaa ataaaaagaa aatggatgct aaatttatat
     1561 caatttgcat ccatttttta ttagttattt attattttgt tgttgattaa ctaattcaag
     1621 atatccactt gggtggaagt aatatatctc agaagtattt tcatttacaa agtaaccaat
     1681 atcgccatct gcatctggta aaaatacata accattaaga tttttagctt ttaatgcttc
     1741 aaaatcacta gcatttcctt gatatatgta atcaacttca ttgttgtatt ttactaaaag
     1801 ttctttagct tgctcttgag tcaatttagt attttggctt acaggaacat tcttttgaga
     1861 atctgcgtaa gaagattcat ttactggtgc agttaataac aaacaagata aaactaataa
     1921 agatataagt gcttttttca tactaaaacc ctctttcatg tttaattata taagattcat
     1981 tacttaataa tattgtatgt ttcctataat taaatattac cattaaagag aataatatta
     2041 cagttacaca gctgtaactt ttgattacaa aatcgtaatt aaattgtatt tttattccat
     2101 ttgtatttta tcatgtctac gatttacaaa ctcacaaaaa tatattttta taacgcccat
     2161 aacaggagca gatagaatca tgcctggtat accaaagaat cctccgccaa cagtaacggc
     2221 taagattgta aagaatgggc taagacctag ttgtccacct acgatttttg gctctataaa
     2281 tgtaacttct acttgttgaa ttattattaa gtaaattaaa gaagttaaag cagttgttgg
     2341 attggagaat aaattaatta ttacaactgg tgccatacct attactggac caaaatatgg
     2401 aaccatattc atgacaccca taattgtacc aaataacagt gcatattcag attttaaaaa
     2461 gtaaagacca attccagaaa gtactccaac tataaaagaa tctaaaatct tacctgtaaa
     2521 atatttacca atatttaaat ttaagctttg acatagttct attataaagt ctccatattt
     2581 tttacctaaa gaaatataaa ctactttctt tgtaaatagt aagaattttt ctttttctaa
     2641 taatatatag aaacaaatta taaaacctaa aagaaattgt actataaact ttccaaccga
     2701 gaatgtagtg ctaaatattc cgccaagtga accaataagt aagttactaa atttaggcat
     2761 tacactcata attttgtcac catattcttt taatgtatta gggtcaactg attgtaaact
     2821 cttgcctaag tcaaataaaa actgttctgt tttatctatg tatgtaggaa tttgagcaac
     2881 taaatcagct aaactattta ctatggatgg aactgtaaat aatataaagg aaattagtat
     2941 aactatgagt acaccataag taagaagtaa tgaatatatt ctcttaaaat ttaatttact
     3001 ttctaagaat tttactagtg ggttgaatat atatgctaat acaaatgcta ttataaatgg
     3061 tgttagtagt gacattaata aacttatcac tccaaaaaaa tatttgtaat tatcaataac
     3121 tttaatcaag atatatgaaa caactacagt gagtacaata tttagataaa tttttttatc
     3181 ttgcaaaata acacaacctt tcttatattt attaaattta gatatactta aatctaagtt
     3241 aaagttaata tttatagctt attatacaat aaaaagcagt attaatatag tctgattaat
     3301 atgaaattat aaatacgtta aaactttatg tttatggcta aaataataaa aattatttat
     3361 attatgttca tatataatct aaaataaaaa ataatgcctt taattaaact aagtttttat
     3421 aatttaaatt ttgtgtaata agctatcttt tattattctt taaaatatat atttcattta
     3481 attatgacaa ataaagataa aaattaaata ttttagaggt aattttattt agatatagaa
     3541 ttttagaata atactataca aatgtattac attattgttt tatatcaaaa atagtaataa
     3601 agtatatata catttagaaa atactgttta aaaggagtag aatttatgat aaaaaaagag
     3661 atatctaatt ttagaggaag tagtgattat gttgtatcac cagaattgat ggcatcagtt
     3721 aatgttgcta ttgcacttga aaaaccactt ttgattaagg gagaacccgg aacaggtaaa
     3781 actatgctag cacaagcaat ttcaaatgaa ttaaaaaaaa atttagtaat ttggaatata
     3841 aaatcaacta caaaagcaca agaggggctt tatgtatatg atactgttca aagactttat
     3901 gatagccaat ttggaggaga aggtgtagat gatattagca agtacataaa atatggtaaa
     3961 ttaggagagg cttttagttc aaatcaacaa gtaattcttt taatagatga aatagataag
     4021 gcggatttag aatttccaaa tgacttgctt tgggaattgg ataaaatgga attttacata
     4081 aatgagacaa aagaaactgt aagagcgaaa caaagaccaa tagttattat aacttcaaat
     4141 gcggaaaaag aacttccaga tgctttttta agaagatgta tatttcatta tatagaattt
     4201 cctgatagag atatgatgga ggaaatagtc aaagtgcatt ttgataaagt agaagaacac
     4261 ctattggaac aagtaatgac tactttttat tggattagaa gtttaaagga tatacaaaaa
     4321 aagccgagta catcagaatt aattgactgg atacaagcat taacattgag tggaatacct
     4381 attgaaaaaa tagaaaaaga agttcctttt gcaggaatac tacttaaaaa taatgaggat
     4441 attgaatcta tgcaaagaca cttatagtta ataggagctg gataattatg tttattgaat
     4501 ttttttattt attgaggtca agaggagtaa atgtatcttt aaatgaatgg atgactttaa
     4561 ttgaagcatt ggataaagga ctgtgctatt caaacttttc aaatttttac tatctttgta
     4621 ggatgattct aataaaaagt gaaagtgatt ttgataagtt tgacacagta tttttagagt
     4681 actttaaagg tatagaacat caagaggaaa ttccagaaga gattatgaat tggcttaaaa
     4741 aaccagatat agatttagaa gagtttgaaa ggttagaaca aaatccaaat ataaatcttg
     4801 acaaattaag agccaaatta gaggaacgaa taaaagagca agattctcct cataatggtg
     4861 gtaattattg gataggaaca gggggaactt ctgagttagg acattcagga aaagggcaaa
     4921 ctggtatacg tataggtgga aattctactt atggtagagc tgttcttgaa gttgcaggag
     4981 aaagaaaata cagagatttt agagatgatg aagttttaaa tatgcgtcaa tttcaagttg
     5041 cactaaagag actgcgtcaa ttttctacta gaatagatgc tccaaagaca gagcttgatt
     5101 tggataagac tattgaagag acttgtaata atgcaggtta tttaaaactg ttttttgaga
     5161 aaccaagaaa aaatacagta aaacttttat tacttatgga ttcaggtggt tcaatgagag
     5221 ggtatagcac tttatgcaat acattatttc agtctgtaag taaatcaaat cattttaaag
     5281 atgtaaagat atactatttt cataactgta tttatgatag attatttaca acacctgaat
     5341 gttggttaag taaatctatt aatactgaat gggtattaaa aaatatagat aaaaactata
     5401 aagttataat tgttggagat gcttctatgt caccttctga gttacttcat attggtggta
     5461 actatcgtgg tccatataat tacacacctg gtatagagtg gcttaaaagg tttaggagaa
     5521 agtacagtaa aactgtatgg atgaatccag aactgagaga tggatgggat tcaattaatt
     5581 attggtatca aactcaaaga atgatacaaa gtgaatttaa tatgtttcca ctgactgtaa
     5641 aaggtcttga gaaagcactg aaaaatttaa tggtttcaag ataaagtaaa tataaatgat
     5701 ggtatgattt attcaaaatt gtaccatcat tatttatttt tttagtatct ctattataaa
     5761 ttatataatt gaaacttaaa aattatatag tctgatacat tttagactta catagaacat
     5821 atacattaat aataatttta gaagtcgagg tgattataat aaattatgaa ttaattgtaa
     5881 aatacaatgg agatatattg agattagaag aagaactagg tgtatcagta gagatactta
     5941 attcatcata tgcaataata acatcaagta atgaagaaga tgtaaataca cttttaactt
     6001 atccagaaat tgaatttata gaaaagcctt ttgtattaca aactcaggat gtacaaagtt
     6061 tttcaagtac aggaataaca gggtttaaaa atagaactgg tttaactggg aaaggaacta
     6121 taattggtat aattgattct ggaatagatt atactttacc tgtgtttaga gatagtaatg
     6181 gtaggtctaa gatattatat tattgggacc agtctattca gggaaatcca ccagaaggtt
     6241 ttagagaagg aacattatac actaatgaag atataaataa tgcaatagat ggaagtatgt
     6301 atatacctat ttcaacaact agtttacatg ggacacatgt tgcaggtatt tgtgcaacta
     6361 ttgcaagtga tgccagaata atagtggtga gagttggaaa tatacaaacc gatatttttt
     6421 caagaagtac agagtttatg agggcaatta aatttatttt agatagagca ttggagttaa
     6481 aaatgcctgt tacattaaat ataagttatg gaagcaatga aggttcacat agaggaacgt
     6541 ctttatttga acaatatata gatgatatgt gtttattttg gaaaaataat atagttgtag
     6601 cggctggaaa taatgcagat aaaggtggtc ataaaaggat tagacttaga aataatatta
     6661 cagaagaagt tgagtttata gttggagaag gagagcgtat actaaatgta aatatatggc
     6721 cagattttgt tgatgatttt agtgtgcatt tagtaaaccc atcaaataat caaacacaaa
     6781 caatatctct aacttcaggt gaaataagga ataccttggg tgaaactaga ataactggtt
     6841 atttttatcc tatagcacca tattctctta caagaagagt tactttgcaa ttaagttcaa
     6901 atacacaaat aactccagga ttatggagaa tcatatttga accaataaat atagtaacag
     6961 gtaatgtaaa tatatattta ccaacttctg aaggactaaa tagaaatact agatttttga
     7021 ttcctactca agagcttact gtaacagttc ctggaacagc aagtagagtt ataacagtag
     7081 gcagtttcaa ttcaagaaca gacatagtat ctatattttc tggagaagga gatacacaat
     7141 taggcgtctt taaacctgac ctattagctc caggtgaaga tatagtatct tttttaccag
     7201 gaggtacaag tggggcatta actggaacta gtatggcaac tcctcatgtt acaggggttt
     7261 gctcattgtt tatggaatgg ggaattgtaa atggaaatga tttatttttg tattcacaga
     7321 aattgagggc attactttta aaagttgcaa gaagattaag caatcaatct tacccaaata
     7381 attcatcagg atttggtttt ttaaatttat cagatgtaga tttgtacact ctatctagca
     7441 taaatcaaga tttagaaaca gaggatattg gatatagaag tattaataag tcatttatgg
     7501 atgaggcaaa taattatata cccatagatg gttataatgt acaaatacat aatgggttag
     7561 agaaggaaaa gtatataagt aaaaatataa gtagacagtc aggaatacta tcaggaatag
     7621 atgtagtcca tactcctgaa tttgaagaag aattagctgg gttaggtcag agtcaaaatt
     7681 ttttaaaaat aagtgattca ttaggtgtac tatctataga taatacagac tataatagca
     7741 tacaacaagt tttacaactt ccttcaatca taagaactat accaactaca aagatgacat
     7801 tgcttggaga aattaataga ggtacttttg gaggagtagt tgcaacagaa gaaataggcg
     7861 taaatttctt taaaaataat cctaatatta atataacagg aagaggtact ctaatatcaa
     7921 ttgcagatac aggaatagac tatctacacc gagattttat ttatccagat ggtacctcaa
     7981 aaatagtata tctatgggac caaacaaaag aaggaaatcc accagatggt ttttatattg
     8041 gtacagaata tacacgagag gatataaatc gagctattgc agcaaatgac cctagtttat
     8101 ctcaggacga agttggtcaa ggaactatgt tgagtggtat atgtgcaggg cttggaaatg
     8161 tgaatagtga gtatgcaggg gtagcagaag attctgaatt aatagtaata aaacttggta
     8221 agatagaagg tttttataat agtgcaatgt tatttgctgc atctcaatat gcatataata
     8281 aagcttttga actgggaaga ccgttagtta taaatgtatc tttaggtacc agtagtttag
     8341 ctggtttaac caatagggtt aatagtgaaa aagcattttt tactagaggt ttatgtgtaa
     8401 cagcaggagc agggaatgag ggcaatacac aaacacatac atctggaata atatcacatg
     8461 ttggaggctc tgttgaagtt gagttagagt taaatgaaga agaggaagaa ttatcattag
     8521 aactttggtt aagtaggcca gataaagctg acgtaataat agtatctcct actggtgaag
     8581 aaagtaaaac tgttggagtt tcaaattata ataaagtaac aggtttattt gacttagaag
     8641 ggactgaata ttctataacg tatatatatc caactacttt ttctggacag caatttacta
     8701 atgttacttt aaagaatgca aaaagaggtg tatggaagat tcgattagta ggggtttata
     8761 ttataacagg gagatataac ttataccttc caaatagaaa acttttaaaa agtggaacta
     8821 gatttagaga ggtagaccct ttttatacta taaattatcc tgctattcaa gatgatttaa
     8881 taactatagg agcttacaat actattaaca gtagtttatg gcaaagttca tcaagaggac
     8941 caactattga aaacagatta aaaccagata tagtagcacc tggtgttaat ataatagctg
     9001 cttatcctgg gaatacctat gcaactataa ctggtacagc agcagcaagt gcacatgcag
     9061 ctggtgctgc agcaatgtat tttcaatata cttttgttga tggtagatat ccaaatcaag
     9121 cctatgttca aaaaataaaa acgtttatgc aagctggagc tagaaaggat tcaaatacag
     9181 tatatccaaa tacaaattct ggatatgggc ttttggatgt tagaggaatg tttgatgttt
     9241 taagatagga gggatttatg gaaaaatctt attgtataat ttatcaaggt gatatagagt
     9301 ctgcactaca agaaaatgga ataaataggt atatggtttt aaatagtcaa cttgcagtaa
     9361 tatatgtacc tatagatttt gatgaaacta ttctaaataa tataatacag gttgcttggt
     9421 gggaagaatc tgcaccaatg agcagtttaa ttgaaataac caataatgta gataatggag
     9481 aaaccattac aacggctgct ggaactgagt atatctatga aaatccatat aatgatataa
     9541 ccggaagagg aattttatta gcagttatag attctggtat agattatttg catcctgatt
     9601 ttataaatga tgacggaacc agtaaagttt tatatttatg ggaccaagaa gcaaatacaa
     9661 atccacctcc agaaggtttt atttttggaa gtgaatttac aagaagtgag ttaaatatag
     9721 ccataaatag aaatgatgga agtttgagtc aagataatat aggtactgga actttagttt
     9781 ctggtatatt agctggaaat ggaagggtaa attcgaagta tagaggtatt actacagaat
     9841 cagacttaat agtagttaaa ttaaaatctt atataggtac ttattatgct ggaaggataa
     9901 attacagtgt ttcagatttt ttagcagcta taacgtatgt tactaatata gcaagaacag
     9961 aaaatagacc actaataata aatctgacta ttggagttaa gtcaggagct gtagcaacta
    10021 cttcaatact agatactttt aatatattga gtagtgctgg tgtagtagta gtgagtggcg
    10081 caggaaatca aggtaataca gatattcact attctggaag atttagtagt gtaggtgaag
    10141 ttcaagatgt gataatacag gatggagatg actatgcact ggatataaca ctaaatacta
    10201 atggtccaga taaagtaggt gcacagataa tatctccttc aggcgaagtt agtcatgaca
    10261 taaggtatgc tccagacttt tatatatata gaggaaaatt taacttagaa aatactactt
    10321 atgcaatgag atttatttat ccatatatta cttctggaaa agaaaatttg gaaataagat
    10381 taagagatat aaaacctgga gtatggactt taagattgac ttcagaactt ataataggtg
    10441 gagaatatga tatatacttg ccaaataaaa atttgatagc tccagataca aggtttttag
    10501 accctgattc agtagctaca ataactatgt atgctgctag tgatgatgta attacagttg
    10561 gtacgttcaa taataagact gacagcatgt ggataggttc ttcaaaaggt ccaatccgag
    10621 gaagaggaat taagccagat atagtagctc caggtgtaga tataatatct acatataaaa
    10681 atggaacgta taatacagga acaggaaccg gtgtgagcag ttctatagtc acaggtgttt
    10741 tagctttact aatggaatat ttggaaaaac agaataatat accaagatta tcattgttta
    10801 cacaagtttt aaagacatat ttgatattgg gagctacaaa gcttgagata tatacatatc
    10861 caaatgtatc acaaggatat gggattttaa acttaaaaaa tacaattcaa caaatagcaa
    10921 atactctata gataataatc tgcataaatt tatagttaat taatagttca gataacaaaa
    10981 aattgaatta gagtaaatat aaaaattaaa aaatagtttt atatgttgta tatataaaat
    11041 aactattttt atataaaaat aattatagat ttagtataaa aaagatatgt tattgactta
    11101 aaaatcatta taattttaac ttatgaattt tatttgaaaa attgctaaaa ctattacaaa
    11161 ataaataata gaggtgatag ttttggaaaa taaaaaagca agagcattaa aaggtaaaga
    11221 taataaaaga cttaaaagtt cacttttagt tggaaatgag tcgacagcag catgggctga
    11281 tgttgaaaaa ttaaaacctc atagtaaagt ttcaatacct tcaattaagg gtgttacaga
    11341 agcaaaagac tgggttgata atggaagtaa attataaaag ttttgaaact gaagtagtaa
    11401 agataagtgt ttattgtcat tttgcaaggt agagttatta tattttaaat agcaatcagt
    11461 atcaattata taaatttatt gactaaaaat ttataaattg gtataatttt gttaattagc
    11521 agaaataata aaagataaat actatgaaga gaagagtaga tataaaatgt agttctagcg
    11581 agttgaggat ggtgaaagct caatacgagg tttatattga agaacatctt ggagtttcta
    11641 atcgaaagct tatttagtaa gtagacttag acggaaggca ccgttatagg ctttaagcat
    11701 tgcttaacta agaggtcatt atgacaacta gggtggtacc gcgagagtaa tagtctttcg
    11761 tccctttata tagggatgaa gggctttttt tattgaaact atatttaaaa gaaattaata
    11821 acaattatac taggaggatt taaaatgcaa aaggaattta taacagtaaa agaattgtat
    11881 agagaaaaag aacagtacat agggaagact gtaaaagtag ctggatggat aagaacgtct
    11941 agagtatcta aaaattttgg atttatagag ctaaatgatg gaagcttctt taaaaatatg
    12001 cagatagtat ttgatgagaa agtagaaaat tttaaagagg taggaaaatt agcaataagc
    12061 tcatctatac ttgtagaggg agaattagta tctacagaag gagctaaaca gccagtagaa
    12121 atacatgcaa aaagcatagt tgtagaaggc gaatcagata gttcatatcc gctacaaaag
    12181 aagagacata cacttgaata tttaagaacg atagcacatt taagaccaag aagtaatact
    12241 ttttcagctg tatttagagt aagatcaata gctgcatatg caatacacaa atttttccaa
    12301 gagagaaact ttgtatatgc acattctcca atcataacag gtagtgactg tgaaggtgca
    12361 ggagaaatgt ttagaataac aactatggat ttaaatgata ttccaaaaac agaagatggc
    12421 aaaatagact atactcaaga tttctttgga aaagaggcta atttaacagt tagtggtcaa
    12481 ttaaatgccg aaataatggc actttctttt agaaatgttt atacttttgg acctacattt
    12541 agagctgaaa actcttatac aggaagacat gcttcagaat tttggatgat agagccagaa
    12601 atagtttttg cagaccttga agataacatg gaacttgcag aagatatgat aaaatatgtt
    12661 ataagctatg ttttagaaaa tgctcctgaa gaaatggaat tttttaatag ttttatagat
    12721 aaaggattgt tagaaagatt aaataagata gtaaactctg agtttgttag aataacatac
    12781 actaaagcag tggaattatt aattgaatct ggtcatgact ttgagtatcc tgttgagtgg
    12841 ggatgtgacc ttcaaactga acatgaaaga tatataacgg agcaaatata taattgtcca
    12901 gtgtttgtta cagattatcc aaaagatata aaagctttct atatgagaat gaatgaagat
    12961 ggtaaaactg ttagagcaat ggacttatta gttcctggag ttggagaaat cgtaggaggt
    13021 tctcaaagag aagaaagaga agatgtatta ctagatagaa tcaatgaaat gggattaaga
    13081 gaagaagatt attggtggta tttagaactt agaaaatttg gtactgctac ccactctgga
    13141 tttggtcttg gatttgaaag aataattatg tatatgacag gtatgtctaa cataagagac
    13201 gttatacctt tcccaagaac tcctaaaaat gctgaatttt aataattata tataataaaa
    13261 cttatataat tttaagtgta aacactaaaa tgacttgcat atttatctat tttagagata
    13321 tgcaagttat ttttattgta ctataaatat ataatttggt tacttttatt ttgcaatata
    13381 atacttatag gtttatgtaa tagtatgtaa ctttttaata aaatgcaaca caattacgtc
    13441 cctgttcttt tgcagtatat agagccttat cacaattact tataaactca tttagcgtta
    13501 ttgtcctttc aattttattt gtgtagactc cgatactaac agttacacat tttaaaggga
    13561 tatcgtcacg ctcaaaatta caggtctcta taatttttcg taaacattct gatttaagtt
    13621 tagcatcttc ctcatcacag ttaaaaatac atataaaaaa ttcctcacca ccatatcgta
    13681 tagcatgttc attttcagaa cttgtaaaat ccaaaattac tttggcaata ttttgtaaac
    13741 aatagtctcc cttttgatgt ccataattgt cattaaattg tttgaaataa tctatatcta
    13801 tcatcattcc agttataggt tcttgatttg catggctgat taattttttt tgtagaatat
    13861 cttcaaggta tctacgatta ttcattttag taaggtcatc tgtcatgact ttgtcgttaa
    13921 gtaaattatt tatatgatat atttcttcat attgttgact aattaaaagt ttgtcatagt
    13981 aagtaaatac acgacttcta tatagcattg tagaaacaaa aacggcaagg gatgttataa
    14041 aaaatgtgtt gacgatatta ctaaactcat tgcctggtat agtttgaata ccaggtaaga
    14101 aaaaattaag aaacaaaaaa catcctccaa aaactattac tgattctcct aattttaata
    14161 ctgagaaagc tgcagctgct agcataatgt aactaaatac acttaggtca tttccaccta
    14221 actggtcaag tagagtaatt acacaacccc ataggcagaa aaaagttaca taaattatcc
    14281 ataaatttat ataaaaagta caacaatcct tttttttatt ccaaagataa gaaaatacaa
    14341 acataaagac agaagataca caaaatagtg ttacatacat ataaaaatac cattggcgtc
    14401 ttggtgtggc aaaaggacct ccctctctag cggagattga tatcatcata attatttgag
    14461 atattactat tattatgcaa atgattttac cagtatggta aagataattg caaagttctt
    14521 tttcaacatt ttctttatgt tttttatcta gaggaaatcc ccaaaatttt ttaatttttt
    14581 ctaagttcat ataagcctcc attaaaaata aagcataaaa ataagtattc ctttatgtct
    14641 ttagatactt ggaatacttt gtttataatt tgattatatc aaaatatatt gtgtgtaaca
    14701 atctaaaatt gtaatggata aaatttatta gctaatgttg aataacttta ttggttaata
    14761 ctttattata attaagattt aaagatagtt tgctactgaa aataaagaat aaaataaatt
    14821 taaattaagc ataataaaat gtaaataata aagttagcat taacaagata tttaaatatc
    14881 aagacctgtt aaaaaacaac agcaataaac aaataaaata gataaatata tccaattaag
    14941 gtataattaa gttttatatg atatagttgt atttaataat ttacaatagt aaaacttgga
    15001 ggggaaaaat gggt
//
LOCUS       DAFNNB010000094        14723 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0095, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000094 DAFNNB010000000
VERSION     DAFNNB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14723
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0095"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            44..1642
                     /locus_tag="L3M60_002382"
     CDS             44..1642
                     /locus_tag="L3M60_002382"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative manganese-dependent inorganic
                     diphosphatase"
                     /protein_id="HBN6227038.1"
                     /translation="MSLLVFGHKNPDTDSICSAISLAHLKNKLGIKATAYALGDIRKE
                     AKYALDYFKVDAPEILDNVRIQVRDLNYDKVVPLTPTSSILEAYNLMDEKNVKTLPVT
                     YDDGTFAGIITMKEIAKNLLHQHFTTIHTSLSNICKNLNGTILVDCGEDVKGRVVTLS
                     FGMETLIETLKEGDIAIVGDRYDSIEYAIDTKVKLLILTNHTEISKDLLALAKINGVS
                     VISVESDNYKTSNVINQCSFLDSIEATENLITFKEDDYMDEIKEVMLETNFRSYPILD
                     DDNKFLGLVSKGHLLNPSKKNVVLVDHNEYAQSADGIEQANIVEIIDHHKLGGISTDV
                     PMSFRVMPVGCNSTIIYQMYKENNVEIPYEIAGLLLSAILSDTLLFKSPTTTDMDKKA
                     CEELSKIAKVDMEKYAMDMFKCGTSLDEYTIEEIVNMDFKEFNMSGHRVGIGQVFTLD
                     IDSIFAKKDEFLSYINSTDYDKLVLAVTDIIKEGSYLIYKAEDSLIANAFGVEGVQGT
                     FAPGVVSRKKQLVPNLTTAIKNFK"
     gene            complement(1733..1945)
                     /locus_tag="L3M60_002383"
                     /pseudo
     CDS             complement(1733..1945)
                     /locus_tag="L3M60_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888191.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
     gene            complement(2574..3098)
                     /locus_tag="L3M60_002384"
     CDS             complement(2574..3098)
                     /locus_tag="L3M60_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN6227039.1"
                     /translation="MENKNPIVTIEMENGKEIKIELYPNIAPNTVKNFVSLVNEGYYN
                     GIIFHRVIPGFMIQGGCPNGTGMGGPGHSIKGEFSGNGFTNNLKHERGVISMARTMAP
                     NSAGSQFFIMHKNSPHLDGQYAGFGRVIEGMDTVDEIASVKTDAADKPQTPQIMKSVT
                     VETFGIDYSDVEKN"
     gene            complement(3163..3915)
                     /locus_tag="L3M60_002385"
     CDS             complement(3163..3915)
                     /locus_tag="L3M60_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBN6227040.1"
                     /translation="MKLGLCLEGGGAKGAYQAGVVKALYDGGINKFYSISGTSIGAIN
                     GYYLYTGNVDNLEKMWTNIKDIQNGNVKIVNNTVDNSPAIDNLRELDDSNIEEMNFYV
                     NYVEVDNKVVSEKIVDVSKMPRNEAIISISYSGLLPSNPNATLGFKEQFVKDVQEGIY
                     DGFKLDGGLIRSALIEPLIGDNVDKIILISTKYNYELPENIKKIYDEDKIIVVRPNTQ
                     FAPKDTLNFDDEFCKTIYQEGYEIGKNILDRL"
     gene            complement(3946..5244)
                     /locus_tag="L3M60_002386"
     CDS             complement(3946..5244)
                     /locus_tag="L3M60_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HBN6227041.1"
                     /translation="MDSINFAKNLIDFIYDSPSSFHTVESTKEILDKNRFEELVLNQR
                     WNLRVGGKYYVTKNLSAIVAFVVNSEDIERDGFRIIGSHSDSPTFSIKPNAEIEAEKS
                     YLKLNTECYGGAILSTWLDRPLGIAGRVVLKGDSILKPNERLIDFKKPICIIPNLAIH
                     LNRSINDGHSYNKQKDMLPLVGLLNDTLEKDNFLIKQIASNLDVNMEDIIDFDLFLYE
                     FEKGSLIGANDEFISIGRQDNLAMVHASLNALVNTKGQHGVNVMAVFDNEEVGSSTKQ
                     GADSNMLLNILERICISIGKDREGFFESIYSSFMISSDLAHAIHPNIPEKHDPTNKPI
                     MGKGPVIKINASQAYTSDGYSIAIYKNICREANVEYQEFVNRSDERGGSTIGPISSTH
                     IDIPSVDVGSPILAMHSIRELGNVEDHYSIYKTFSKFFEI"
     gene            complement(5261..7525)
                     /locus_tag="L3M60_002387"
     CDS             complement(5261..7525)
                     /locus_tag="L3M60_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3553 domain-containing protein"
                     /protein_id="HBN6227042.1"
                     /translation="MNLDTLNPAQREAVEKTEGPVLILAGAGSGKTKVLTTRIAYLIE
                     DKGVQAPNILAITFTNKAANEMRERVEQNIGPETKDMWISTFHSCCVRILRKDINKIG
                     YNRSFVIYDSADQVTLVKDCLKELNLSDKVFEPKAVISAISGAKDKLYTPKQFKDINM
                     ADNRMVKIADIYALYQDRLKRNSALDFDDLILKTVELFKANDEVLAYYRSRFRYIMVD
                     EYQDTSKAQYELIKLLAREHQNICVVGDDDQSIYGWRGADIRNILEFEKDYDNVHVVK
                     LEQNYRSTQVILDAANKVISNNIERKRKKLWSEKKEGELIKIQLTGSEIEEADFIADS
                     IAQIARKENRPYKDFAVLYRANAQARPVEDALNRSQIPYNIYGGTKFYERKEIKDLLA
                     YLRVIQNPQDDISIKRIINVPRRGIGLRTIEKIEDRANLKQESIYSVLIDIETNSDIS
                     TKARASISGFVDIIGTLRTIKEVYPVSKLIEKVLDTTGYMDELVEIRNKNEKDLTGKG
                     EEAQDRIDNLREFISIALEFESSNDDTYENKDLETFLTSIALTSESNDEEDNDRVSLM
                     TIHTSKGLEFPVVFLTGMEEGLFPISRAIKSMSDSQIEEERRLCYVGITRAKEELYMS
                     LTEKRTLYGKTNVAIASRFMEELPEECIERLYKVKKELSYSKASYNMLDKYTKKYMST
                     ISKSKVADKVNATIKDSNKETNPDDIKLGSKVHHPKFGVGTVVSIIGTDVTIAFDQQG
                     IKKINKEYTTLNIL"
     gene            complement(7779..8597)
                     /locus_tag="L3M60_002388"
     CDS             complement(7779..8597)
                     /locus_tag="L3M60_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBN6227043.1"
                     /translation="MFKINNLTYQYEKNANALLNINMDFSKGNVIGIIGSNGSGKSTL
                     FMNLMGILKPTSGEIFFKEEKLKYDKKSLYNLRKNVGIVFQDPEKQIFYSKVYDDIAF
                     AMRNIGIDEKTIKERINKALVAVNGIDFIDRPVHFLSYGQKKRVAIASVIAMENEIVL
                     LDEPTAGLDPVSTRSIVDIIKGLNKNNIKIVISSHDMNLMYEICDYIYVLDKGILIDE
                     GKAENVFINENNIIQAGLESPWLVKVHKNMNLPLFKKEEDLYKYWKERELNTNK"
     gene            complement(8590..9288)
                     /gene="cbiQ"
                     /locus_tag="L3M60_002389"
     CDS             complement(8590..9288)
                     /gene="cbiQ"
                     /locus_tag="L3M60_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HBN6227044.1"
                     /translation="MLIIDKYAYTNRLSKVNPNKKVAIGVIFLIASMVIQNIFILSGI
                     MILMSILVVCVAGIDLKNYLKLLRIPMYFLFLSIGITLVNISFNKADLLHSFEIFSFN
                     VGISKASIDMSIHVLFRAMSCLTCVYFCILTTPFNQLIFFFKKLHLPDTFVELSMLIY
                     RFIFIFLEEFSEIYKSQELRFGYINLKTSYKSLGILGSMLYKRLMTRYDDMCISLDIK
                     LYDGKFHIVGDNDV"
     gene            complement(9291..9575)
                     /locus_tag="L3M60_002390"
     CDS             complement(9291..9575)
                     /locus_tag="L3M60_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6227045.1"
                     /translation="MSAKTKTKTNILLLLLVVALIIFPLLVNSGAEYGGADGQAESEI
                     TKINPNYKPWFSSPYEPPSGEIESLLFSAQAALGAGVIGYILGLQKGKRS"
     gene            complement(9565..10317)
                     /locus_tag="L3M60_002391"
     CDS             complement(9565..10317)
                     /locus_tag="L3M60_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HBN6227046.1"
                     /translation="MKQNIKLGVIAALMLIILTPVTSNAMHIMEGYLPVKWSIAWGVI
                     FIPFFLVGLKSIGKIVKQDPKKKVLLALCGAFVFVLSALKIPSVTGSCSHPTGVGLGA
                     IMFGPSVMFVLGTIVLIFQALLLAHGGITTLGANAFSMAIIGPIVSFLIFKALKKKDG
                     NNAMPVFLAAALGDLATYTITSIQLALAFPDPSGGVMASAIKFLGIFFMTQIPIAIAE
                     GILTVIVYNLITENGEKSILENNDKGVKANEC"
     regulatory      complement(10504..10696)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(10828..12600)
                     /locus_tag="L3M60_002392"
     CDS             complement(10828..12600)
                     /locus_tag="L3M60_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation-like protein"
                     /protein_id="HBN6227047.1"
                     /translation="MKWIYDDYEFENRANNLSWTIAGDYNESIDISEKDYSSKEIGLY
                     FAIIAGARRKYVDWDIVKKYLTNRIRKGYNKDIILGLIQVILNTIVEEKAIKDRPGIE
                     DIRRNAYKDIVSRFTKIHNDNIMDKIKYAFVLYSMDKHPALDSMTRRIVNDIRNIDTK
                     QDIMEVLKELDVIYLTYFEYILNEEESSLIDENNVKNVEVDFDTFADFMYEELYSDEE
                     ENIIESDIDSISSSMLVEGVGDFDDSKSSNLADRVIYVDEDTANKIYSKIEHYYGKTY
                     LSKGEIKKIETQNCRSVHEGCRIHFTDGVLRSECNNVFQLKYVTRQKENNLGKFREHA
                     RMYRQQIKRLKESLSRILIEENAKTRVYSDCGTILANRAWRVGRSNNNKIFYKDIENE
                     KGKYVIDILLDASGSQSRNQGNVAIQAYIIANALTEVGIPNRVMGFSSFLDYTILKRF
                     KDYDDSLKNSENIFEYFCAGNNRDGLAIKSICSGLINRYEENKILIVLSDGRPNDIKI
                     GKSSERTLRGEKAYRGIVGLRDTANEVRKARKQNILVLGVFTGKESELEAERLIYGND
                     FIYSRDITRFSDVVSMYLKKIIRN"
     gene            complement(12614..13543)
                     /locus_tag="L3M60_002393"
     CDS             complement(12614..13543)
                     /locus_tag="L3M60_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HBN6227048.1"
                     /translation="MNIIDNLRAQGVDEKLIEDVMYFRNYYGLEKDLEYRVTRSKTYF
                     YGKDILSMCIAAILEEENILLSGPKATGKNLLADNLGEIFNRPQWNTSFHINTDSSTL
                     IGTDTFIDNEVKLRRGSVYECAINGGFGVFDEINMAKNDAIVVVHSALDYRRIIDVPG
                     YERVNLHPATRFIGTMNYEYAGTKELNEALVSRFMVIDIPPIEEDKLMMILKNEFSDA
                     DEEKLIQFAGIFLDLQLKSQNGEISSKAIDLRGLMASLKTIRRGLKPTLAINMGLTGK
                     TFDVYEKEMVGDVIKTRIPNKWESIDVFPISHI"
     gene            complement(13949..14557)
                     /locus_tag="L3M60_002394"
     CDS             complement(13949..14557)
                     /locus_tag="L3M60_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBN6227049.1"
                     /translation="MSVINSFILQYGLISVFVLIMIEYACFPLPSEVVLPLCGAIAAR
                     NHFGFLTILILSIIAGILGSIFCYTVGNWGGKSIINKIIEICPKAKKGIFASQDYFNK
                     YSSISVCVCRLIPLCRTYISFIAGIAGQNIITFVVSSIVGITIWNTSLISIGYIFSES
                     WVRIMSHYNDYKFLVLIIPISIIFIGFVIKSFIHKKHHKIRL"
BASE COUNT         4825 a         2524 c         1626 g         5748 t
ORIGIN      
        1 ttatcaatta ttattatatt caagttttga aaggagtatt aaaatgagtt tattagtttt
       61 cggacataaa aatccagaca cagattcaat atgttctgca atttcattag cacatttaaa
      121 gaacaaacta ggaataaaag caacagctta tgctttagga gatatcagaa aagaagctaa
      181 atatgccttg gattacttta aggtagatgc acctgaaatt ttagataatg ttaggataca
      241 agttagagat ttaaattatg acaaagtagt acctttaaca ccaacttcat ctatattaga
      301 agcctataac cttatggatg aaaaaaatgt taaaacacta cctgtaactt atgatgatgg
      361 aacttttgct ggtatcatta ctatgaaaga aatagctaaa aacctacttc atcaacactt
      421 tacaactata cacacatcac tatcaaacat ttgtaaaaac cttaatggta ctattcttgt
      481 ggattgtggt gaagatgtaa aaggtagagt agttacttta tcatttggaa tggagactct
      541 aatagagact ttaaaagagg gagatatagc tattgttggc gatagatatg atagtattga
      601 atatgctatt gatacaaaag ttaaactttt aatacttact aatcatacag agatttcaaa
      661 agacttgcta gctcttgcta agataaatgg agtgtctgtt atttctgttg aaagtgacaa
      721 ttataaaact tctaatgtaa taaatcaatg tagtttctta gactctatag aagctacaga
      781 aaatttaatt acattcaaag aagatgatta tatggatgaa ataaaagagg ttatgctaga
      841 gacaaacttc agatcttatc caatattaga tgatgataat aagtttttag gtttagtttc
      901 aaaaggtcat ctattaaacc catctaaaaa gaatgttgtt ttagttgacc ataacgaata
      961 tgctcaaagt gctgatggaa ttgaacaagc aaacatagtt gaaataattg accatcataa
     1021 acttggtggt atttctactg atgttcctat gtcatttaga gttatgcctg ttggatgcaa
     1081 tagtactata atttatcaaa tgtacaaaga aaataatgtt gagatacctt atgaaatagc
     1141 tgggcttctt ttatctgcta tattatcaga tacattatta tttaagtctc caacaacaac
     1201 tgatatggat aagaaggctt gtgaagaatt aagtaaaata gcaaaagtag acatggaaaa
     1261 atacgctatg gatatgttta aatgtggtac ttctttagat gaatatacta tagaagaaat
     1321 agtaaatatg gattttaaag agtttaatat gagtggacat agagttggta ttggacaggt
     1381 atttacttta gatatagact ctatatttgc taaaaaagat gagtttttat cttatataaa
     1441 ttcgactgat tatgataaat tagttcttgc agttacagat ataatcaaag aaggttctta
     1501 tttaatctat aaagcagaag atagcttaat tgctaacgca tttggtgttg aaggggttca
     1561 aggtacattt gcaccaggtg ttgtatcaag aaaaaaacaa ttagttccta atttaactac
     1621 tgcaatcaaa aattttaaat aatataaagt ttttaaacaa taaaaaagaa ggtgtctcaa
     1681 agtgaatttt atagtccatg aggcactctt ttttgtataa aatcaaatat attattctaa
     1741 atatcatcaa agtagccgta attaccgctg attgcccatt tcgtattcct actgctatag
     1801 ataaaaaatt atttatagta aaagtagttc ctccaaataa aaagccagcg aaatacccag
     1861 aaagtactca tcatcatgat tcagtagtaa gacctgttaa atctcttcct cctagaatta
     1921 gaaataaaat agcgaaagaa tacatactta gttgcaacat aattaaaatt tacagtctta
     1981 aatttttata ttaataatgc taatttccac aaaaaaagaa catgtaatta gaatcttcta
     2041 acagcatgtt ctttttcatt attatatatt acactttaaa ttattccttg actggcttag
     2101 ctggtgtaaa ctaaaccagt ctttctaaaa tattaagcac ttttttctta ttatgttttt
     2161 taggatttat tgtctatatt attgtaaacc aaagttataa aattgtaaat agaatatatt
     2221 accatatcaa tattttttta taagtaaaaa ttataaaaaa acttattttt attaaataaa
     2281 atatctaaaa taagtttaaa agatactgaa ataagtgttt tccacatatc ttattatatt
     2341 tatttaatac attattattt aaaaataaag atataagcaa tcatagtaaa atttttttaa
     2401 tagaggttta ttttttagtt aaaatcctac aatatatagt tttctctaat acaaaaaaat
     2461 taaaaagtga aatgtatttg tctaaaaaca taacaacatt tcactttatt ctcattatta
     2521 agtaatatat ttttaatttt tatttataac tcaatctagt ttttatataa gttttagttt
     2581 ttttctacat ctgaataatc tattccaaaa gtttctacag taacagattt cataatttgt
     2641 ggagtttgtg gcttatcagc tgcatctgtt ttaactgatg ctatttcatc aactgtatcc
     2701 ataccttcta tcactctacc aaaacctgca tattgaccat ctaagtgtgg tgagttttta
     2761 tgcattatga aaaattgaga accagctgaa ttaggtgcca tagttctagc cattgatatg
     2821 actcctcttt catgttttaa attgttagta aatccattac cactaaattc cccttttatt
     2881 gagtgacctg gaccgcccat tcctgtacca tttgggcacc ctccttgtat cataaatcct
     2941 ggtattactc tatgaaatat tattccattg taatatcctt catttactaa tgatacaaag
     3001 tttttaactg tatttggagc tatatttgga tataactcaa ttttaatttc ttttccattt
     3061 tccatttcta tagttactat aggattttta ttttccataa tttattacct tccttttcat
     3121 tagttaaatt acataattac atttattata ttatactcta aattaaagtc tatcaagtat
     3181 attttttcct atttcgtatc cttcctgata aatagtttta caaaactcat catcaaagtt
     3241 aagtgtatct ttaggagcaa attgagtgtt aggtctaact actattattt tatcttcatc
     3301 gtatattttt ttaatattct ctggcaactc ataattatat tttgtggata ttaaaataat
     3361 tttatccaca ttatccccaa ttaatggttc aattaatgca ctcctaatta aacctccatc
     3421 taatttgaaa ccatcataaa ttccttcttg cacatctttc acaaattgtt ctttaaatcc
     3481 aagagttgca tttggatttg atggcaataa cccactgtat gaaatactga taattgcttc
     3541 attacgaggc atttttgata catcaacaat cttttctgat actaccttat tatcaacttc
     3601 aacataatta acataaaagt tcatttcttc tatattacta tcatctagtt cccttaggtt
     3661 gtctatagca ggagaattat ccacagtatt atttactatt ttcacattcc cattctggat
     3721 atcttttata tttgtccaca ttttctccaa gttatcaaca ttccctgtgt acaaataata
     3781 tccatttatt gctcctattg atgtccctga aatagaataa aatttattta ttcctccatc
     3841 atacaaggct ttaactactc ctgcttgata agcccctttt gctcctccac cttctaagca
     3901 taatcctaat ttcatagtct catctccaaa ttgtttttct tatatttata tttcaaaaaa
     3961 cttactaaat gttttatata tactatagtg gtcctcgaca tttcctaatt ctcttataga
     4021 gtgcattgct aaaattggac ttcccacatc cacagatggt atatctatat gagtagaaga
     4081 tattggacct atagtacttc cacctctttc atctgaccta ttaacaaatt cttgatattc
     4141 aacattagct tctctacata tatttttata aatagctata gaatatccat cactagtata
     4201 tgcctgacta gcatttatct ttataactgg accttttccc ataattggtt tattagttgg
     4261 gtcatgcttt tctggaatgt ttggatggat agcatgagct aaatctgatg atatcataaa
     4321 agatgaataa atagattcaa agaatccttc tctatcctta cctattgata tgcaaatcct
     4381 ctccaatata tttaacaaca tgtttgaatc tgcaccttgt ttagtagagc taccaacttc
     4441 ttcattatca aatacagcca ttacatttac accatgttga cctttagtat taacaagagc
     4501 atttaagcta gcatgtacca ttgcaagatt atcttgtctc ccaatagata taaattcatc
     4561 attagcacct attaaacttc ctttttcaaa ttcatataaa aataaatcaa aatctataat
     4621 atcttccata tttacatcta agttagatgc tatttgcttt ataagaaaat tatctttttc
     4681 taatgtatca tttaacagcc caactaaagg taacatatct ttttgcttat tatatgaatg
     4741 accatcattt atgcttctat ttaaatgtat cgctagattt ggtattatac aaattggctt
     4801 tttaaagtct ataagccttt catttggttt taatatacta tcacctttta aaactactct
     4861 accagctatc ccaagaggtc tatccaacca tgtacttaat attgctcctc catagcattc
     4921 tgtatttaac ttcaaataag atttttcagc ttctatttct gcatttggtt taatactaaa
     4981 tgtaggagaa tctgagtgag agcctattat tctaaatcca tctctttcta tatcttctga
     5041 atttactaca aaagctacta tagctgataa attcttagta acataatact ttccaccaac
     5101 ccttagattc catctctgat tcaaaactaa ttcttcaaat ctatttttat ctaagatttc
     5161 cttagtactc tcgactgtat gaaaagacga cggactatca tatatgaagt ctattaagtt
     5221 tttagcaaag tttatactat ccattcctca tttacctcca ttataaaata tttagtgttg
     5281 tatattcttt atttattttt ttaattccct gttggtcaaa agctatagta acatctgtcc
     5341 caattataga aactactgtc ccaaccccaa atttaggatg atgtacttta gaaccaagtt
     5401 ttatatcatc tggattagtt tctttattag aatcttttat tgtagcatta actttatctg
     5461 caactttaga tttacttata gtactcatat actttttagt atatttatct aacatattat
     5521 aacttgcttt agaatagctt agttcttttt ttactttgta caatctttct atacattctt
     5581 ctggcagttc ttccataaat ctggaagcta ttgcaacatt tgttttacca tataatgttc
     5641 tcttctccgt caatgacata tatagctctt ctttagctct agttatacca acataacaaa
     5701 gtcttctctc ttcttctatt tgtgaatcac tcatagattt tatagctctt gatataggaa
     5761 ataatccttc ttccatacct gttaaaaaca caacagggaa ctctaaacct ttggatgtat
     5821 gaattgtcat aagcgaaacc ctatcattgt cttcctcatc atttgattca gatgtaagtg
     5881 ctatacttgt taggaatgtc tctaagtctt tattttcata agtatcatca ttactacttt
     5941 caaactccaa agctatagat ataaattctc tcaaattatc aattctgtct tgagcttctt
     6001 cacctttacc tgttaaatct ttttcatttt tatttcttat ctctacaagc tcgtccatat
     6061 atcctgtagt gtccaaaact ttttctataa gtttactaac aggataaact tcttttatag
     6121 ttctaagagt ccctataata tcaacaaatc cacttatact tgctcttgcc ttggtagata
     6181 tatctgaatt tgtttctata tcaattagta ctgagtatat gctctcttgt tttaaattag
     6241 ctctatcttc aattttttct atagttctaa gccctatacc tcttctagga acatttataa
     6301 tcctttttat agatatatca tcttgtggat tttgaataac tcttaaatac gccaacaaat
     6361 cctttatttc ttttctctca tagaattttg taccaccata tatattgtat ggaatttgac
     6421 ttctatttaa agcgtcctct acaggtcttg cctgtgcatt tgctctataa agtactgcaa
     6481 aatccttata aggtctattt tcttttctag ctatttgtgc aattgaatca gcaatgaaat
     6541 cagcctcttc tatttcgcta ccagttaatt gtatctttat aagttctcct tctttttttt
     6601 cactccaaag cttttttctt tttctttcta tattattaga tataacttta ttagctgcat
     6661 ctaatatcac ttgtgtagac ctataatttt gttctaattt tacaacatgt acattgtcat
     6721 aatctttctc aaattcaagt atgtttctta tatctgcgcc tctccatcca taaatacttt
     6781 ggtcatcatc tccaacaaca caaatatttt gatgttctct tgctaataat tttatcaatt
     6841 catattgagc tttacttgta tcttggtact catctaccat tatgtatcta aatctacttc
     6901 tataatatgc taacacttca tcattagctt taaaaagttc tacagtttta agtattaaat
     6961 catcaaagtc aagtgcacta tttcttttta aacggtcttg atataacgca taaatatctg
     7021 caatcttaac cattctatta tctgccatat tgatatcttt aaactgcttt ggagtataaa
     7081 gtttgtcttt tgctcctgaa attgcactta taactgcttt tggttcaaac actttatcac
     7141 ttaaatttaa ttcttttaga cagtctttaa ccaatgtaac ttggtctgct gaatcataaa
     7201 ttacaaaact tctattgtaa ccaatcttat ttatatcttt tcttagtata cgaacacagc
     7261 aagaatggaa tgtacttatc cacatatcct tagtttcagg acctatgttt tgttcaactc
     7321 tttctctcat ttcatttgca gctttgtttg taaatgttat agctaatata tttggtgctt
     7381 gtacaccttt atcttctata agatatgcta ttcttgttgt taatacctta gttttacctg
     7441 aaccagcacc agccagtata agtactggac cttcagtctt ttctactgct tctctttgag
     7501 caggatttaa tgtatctaaa ttcatatttc tcctcctgtt aacagccata aatatatttt
     7561 aatctaaaat atgaattact tcaaattata attatgtatt atatcaatag attatttcca
     7621 atatattaca attaaataac atttttccat ataatttatt tcattgttta ataaaaaaaa
     7681 taaccttatg atttggggaa tcataaggca cctttaaggg gtctaggtta agggttatta
     7741 tatataaatc tattttttta aaataggttt agggctaatt atttatttgt attaagttct
     7801 ctttctttcc aatacttata caaatcttct tctttcttaa ataaaggtaa attcatattt
     7861 ttatgtactt ttactaacca tggggattct agaccagctt gtataatatt attttcattt
     7921 ataaatacat tttctgcctt tccttcatca atcaaaatcc ccttatccaa tacatatatg
     7981 taatcacata tttcatacat taaattcata tcatgactgg atattactat ttttatatta
     8041 tttttattta atccctttat tatatctacg atagacctag tgcttactgg gtctagacca
     8101 gctgtaggtt catcaagtaa tacaatttca ttttccattg ctataactga tgctattgcc
     8161 accctttttt tctggccata actaagaaaa tgtactggtc tgtctataaa atctattcca
     8221 ttaacagcta ctagagcttt gtttattctc tctttaattg ttttttcatc tataccaatg
     8281 tttctcattg caaatgctat atcatcatat acttttgaat aaaaaatttg tttttctggg
     8341 tcttgaaaca caatacctac attttttctt agattgtata aacttttttt atcatacttt
     8401 aacttctctt ctttaaaaaa aatttctcca gaggtaggct ttaatatccc cattaagttc
     8461 ataaataatg ttgattttcc tgaaccattt gaacctatta tacctataac atttcccttt
     8521 gaaaaatcca tattaatgtt taataatgca ttggcatttt tttcatattg atacgttaaa
     8581 ttatttattt taaacatcat tatcccctac tatgtgaaat ttaccatcat acaattttat
     8641 gtctaatgat atacacatat catcatacct tgtcataagt cttttgtaca gcatacttcc
     8701 aagaattcct aaggatttat atgaagtttt taaatttata taaccaaatc ttagctcttg
     8761 tgacttgtat atttctgaaa attcttctaa aaatatgaat ataaacctat atattaacat
     8821 tgataactca acgaaagtgt caggtaaatg aagtttttta aaaaagaata ttaattgatt
     8881 aaaaggtgtt gtaagtatac aaaaatacac acaagtcaaa caagacatag ctctaaataa
     8941 cacgtgaata gacatgtcaa tagacgcctt tgatattcct acattaaaac taaaaatttc
     9001 aaaactatgt aataaatctg ctttattaaa agatatattt acaagagtta ttcctatact
     9061 aagaaataaa aagtacattg gtatcctaag taattttaaa tagtttttta aatctattcc
     9121 agccacacaa acaaccagaa tactcattaa aatcataatt ccacttaaaa taaatatatt
     9181 ttgtattacc atagaagcaa tcagaaagat tactcctatg gctacttttt tgtttggatt
     9241 tactttactt aatctatttg tatatgcata tttatcaatt ataagcatga ttaacttctc
     9301 tttccttttt gaagacctaa tatgtatcct attacaccag cacctaaagc tgcttgtgca
     9361 gagaataata aactttctat ttctccactt ggtggctcat atggactgct aaaccatggt
     9421 ttgtaatttg gatttatctt agtaatttca ctttcagctt gaccatctgc tcctccatat
     9481 tcagcacctg agttgacaag taacgggaat ataataagag ccactactaa taaaagtaat
     9541 aatatgttag tttttgtttt tgttttagca ctcattagct ttaactcctt tatcattatt
     9601 ttctagtatg cttttttctc cattttctgt aattaaatta tatacaatta cagtaagtat
     9661 accctctgct attgctattg gtatttgagt cataaagaaa attcctaaaa acttaattgc
     9721 tgaagccatc actccacctg atggatctgg aaaagctagt gctaactgta ttgaagtaat
     9781 tgtatatgta gccaaatctc ctagtgctgc tgcaagaaat actggcattg cattatttcc
     9841 atcttttttc tttaatgctt taaatattaa aaatgataca attggtccta taatagccat
     9901 tgaaaaagca ttagctccta aagtagttat gcctccatgt gctaatagta atgcttggaa
     9961 tattaaaact atagttccaa gaacaaacat tacgcttggt ccaaacatta tagctccaag
    10021 acctactcca gtaggatgtg agcaacttcc tgttactgaa ggtattttta gtgctgacaa
    10081 tacaaacaca aatgctccac atagcgctaa taatactttc tttttagggt cttgctttac
    10141 tatttttcca attgatttta gtcctactaa gaaaaatggt ataaatataa ctccccatgc
    10201 tatagaccat tttactggta aataaccttc cattatgtgc attgcatttg atgtaactgg
    10261 agttaaaatg attaacataa gtgcagctat aacccctaat ttgatatttt gtttcatttt
    10321 tagtatcccc tctctccctt tctaataatc aaaatataag tgatacatgt ctgatttatt
    10381 ttaaataaaa taaaaaaacc taactcgatg gttaggcttc taattttcca caatccaaat
    10441 acaaacacat aaaacaaaca tatctgtata attattataa gaaactttct ccatccccga
    10501 agaaattact ttaatatttt aggcaggtct cctgacttag cttcttccta atcctcttcc
    10561 ttcccatgag atataaaaaa tatcatcata gtggctaaca aaagtttcgt ctgcatcaca
    10621 gtagcggggg ctgtaaagga atttaacctt tttccctatt aatcttgctt gaagaaccta
    10681 taatattata tatttaattt ttaaattatt cttaaattgt attctatata aatcattgta
    10741 taccttttct gtgaaaattt ctacattttt tataaatttt attcattttt atatttttat
    10801 gtatataaat tcttttttac aaacttatta gtttctaatt atcttcttta aatacattga
    10861 aaccacatca gaaaatctag ttatatctct tgaatatata aaatcatttc catatatcaa
    10921 tctttcagct tcaagttctg attctttacc tgtaaacact cctaatacta atatattttg
    10981 ctttctagct tttctcactt cattggctgt atctctcagt ccaactatac ctctataagc
    11041 tttttctcct ctcaaagtcc tctcactact ttttcctatc tttatatcat ttggtcttcc
    11101 atcactaaga actatcaaga ttttattttc ttcatatcta tttattagac cactacatat
    11161 agattttatt gccaaaccat ctctattgtt accagcacaa aaatattcaa atatattttc
    11221 actatttttt agactatcat cataatcttt aaatcttttt aatatagtat agtctaaaaa
    11281 actagaaaat cccattactc tatttggtat gccaacttct gttagagcat ttgcaattat
    11341 gtaagcttga attgccacat ttccttgatt tctactttga gagccacttg catccaataa
    11401 tatatcaata acgtatttcc ctttttcatt ttctatatct ttataaaata ttttattatt
    11461 attacttcta ccaactctcc aagctctatt tgctaatata gtaccacaat cagaatatac
    11521 tctagtttta gcattttctt ctattaaaat tcttgacaga ctttctttta atctctttat
    11581 ttgctgtcta tacattctag catgttctct aaattttcct aaattatttt ctttttgtct
    11641 tgttacatat tttagttgaa atacattatt acactcactt ctaagaactc catctgtaaa
    11701 gtgtattcta caaccttcat gaacacttct acaattttgt gtctcaattt ttttaatttc
    11761 acctttactc aaataagttt ttccataata atgttctatt tttgaatata tcttatttgc
    11821 agtgtcttca tcaacatata taactcggtc tgctaaattg ctagatttgg aatcatcaaa
    11881 atcccctaca ccttctacaa gcatggatga agatatcgaa tctatatcac tttcaataat
    11941 attttcttct tcatcactat aaagttcttc atacatgaaa tcagcaaatg tatcaaagtc
    12001 tacctctaca ttttttacat tattttcatc aataagacta ctttcttctt catttaatat
    12061 gtattcaaaa taagttaaat atataacatc taattctttt aaaacttcca taatatcttg
    12121 tttggtatct atatttctta tatcattaac tattcttcta gtcatactat ctaatgctgg
    12181 atgcttgtcc atactataaa gtacaaatgc atatttaatt ttatccataa tattatcatt
    12241 gtgtattttg gtaaaacgtg aaacaatatc tttataagca tttctcctta tgtcttctat
    12301 tcctggtcta tctttaatag ccttttcttc aactattgta tttaatatta cttgaattag
    12361 acccagtata atatctttgt tgtatccttt tcttattcta tttgtaagat attttttaac
    12421 tatatcccaa tcaacatact ttcttctagc acctgcaatt attgcaaaat acagacctat
    12481 ttcctttgaa gaataatctt tttctgatat atctatactc tcattgtagt cgccagctat
    12541 tgtccaggat aagttattgg ctctattttc aaattcataa tcatcatata tccatttcat
    12601 aatctcacct atattaaata tgacttattg gaaacacatc tatactctcc catttattag
    12661 gtattctagt tttaattaca tctccaacca tttctttctc atacacatca aaagtctttc
    12721 ctgtaagccc catattaatt gcaagggttg gttttaagcc tcttcttatg gtcttaagag
    12781 atgccataag accacgtaaa tcaatagctt tactagatat ctcgccattt tgagacttca
    12841 attgtaaatc taaaaatatt cctgcaaatt gtattaattt ttcttcatca gcatctgaaa
    12901 attcattttt taaaatcatc attaacttat cttcttctat aggaggaata tctattacca
    12961 taaatctaga taccaatgcc tcattaagtt cttttgttcc tgcatattca taattcatag
    13021 tcccaataaa tcttgttgca ggatgtagat ttactctctc atacccaggt acatcaatta
    13081 tccttctata gtctaaagct gaatgtacca caactatagc atcatttttt gccatattaa
    13141 tttcatcaaa taccccaaaa cctccattaa tagcacactc ataaactgaa cctcttctaa
    13201 gtttaacttc attgtctata aacgtatctg tacctataag tgttgaacta tctgtgttta
    13261 tatgaaatga tgtattccac tgaggtctat taaatatttc ccctaaatta tcagcaagta
    13321 ggttttttcc tgttgccttt ggtcctgata ataatatatt ttcttcctct aaaatcgctg
    13381 ctatacacat agataatatg tcttttccat aaaaataagt tttagacctt gttactctat
    13441 attctaaatc tttttctaga ccataataat ttctaaaata cattacatcc tcaataagtt
    13501 tttcatctac cccttgtgcc cttagattat ctattatatt cattcttctt acccctttta
    13561 tataaatagt atttgtttca tttcttaaca ccattttagc ataaaaggat ataagctaga
    13621 attgatttca taaattaatg acaactcttc ctctctttta aaaaaaacta cctatatgta
    13681 ctttttatgt attatttttg ttaaatgtct gaaagttatc agattactat tcattacagg
    13741 atttataagt tgaggcttgt gaatgtattt gattactata tattgttcta gttttgaatt
    13801 tattatcttg acaatatttc atctgtttta catcaaaaat ccccgctttc atatagaact
    13861 agaaacgggg attttgatta ttataaattt tctcaaacat atgaacttta gaatagctgt
    13921 agaaaaagat acaaaacttt ttcatttctt ataatcttat cttgtgatgt tttttatgta
    13981 taaatgactt aataacaaat ccaataaaaa ttatggatat tggtataatt agaactagga
    14041 atttataatc attataatgt gacattattc ttacccaact ctcagaaaaa atatacccta
    14101 tactgattaa tgaagtattc catatggtta ttcccacgat agatgatact acaaaagtta
    14161 taatattttg acctgctata cctgctataa aagatatgta tgttctacat aaagggatta
    14221 atctacatac acaaacagaa atagaagagt atttattaaa ataatcttga gatgcaaata
    14281 ttcctttctt tgcctttgga catatctcta ttattttatt tataatagat ttacctcccc
    14341 aatttcccac tgtataacaa aatatcgaac caagtatacc tgcaattata cttaaaatta
    14401 aaattgtcaa aaatccaaaa tgatttcttg cagcaattgc tccacataaa ggtaaaacaa
    14461 cttcgctagg taatggaaaa caagcatact caatcataat taatacaaat acagagatta
    14521 atccatattg taaaataaaa ctattgatta cagacactta cttatctatc tcctttctta
    14581 tttaaaggtt ttaagggtac atattctaag gattctcacc ctatatttaa agaatcaatc
    14641 tctttaaact attatatgta tttacagaaa gcatttataa catactttaa aaatagtacc
    14701 ttaaaataag aaacttaata aat
//
LOCUS       DAFNNB010000095        14679 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0096, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000095 DAFNNB010000000
VERSION     DAFNNB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0096"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            58..399
                     /locus_tag="L3M60_002395"
     CDS             58..399
                     /locus_tag="L3M60_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator"
                     /protein_id="HBN6227050.1"
                     /translation="MKEENFINIEYEDKRFSINSKEVLYIEKQEYEVKIYTENGNYEV
                     GENAPCIRKLMKEENFFKCSNSYIVNLDKCEFYLSNNCIFVNNNFIRILGSERKKKLK
                     KKLEKLYDYED"
     gene            550..963
                     /locus_tag="L3M60_002396"
     CDS             550..963
                     /locus_tag="L3M60_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
                     /protein_id="HBN6227051.1"
                     /translation="MLELKNNSGTSERKLKYPCIYKHFKGKYYATMGLSKAIDNVENI
                     CEIYSKENLIQNRNKYKLVIRHTEREEDIYVYRDLDGNFYHKKEEDTNDLVLYKTLYD
                     DTGIFGRPLDMFLEKVDTDKYVNSIQEYRFEEVYK"
     gene            1163..1804
                     /locus_tag="L3M60_002397"
     CDS             1163..1804
                     /locus_tag="L3M60_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBN6227052.1"
                     /translation="MGNTFYIVRHGQTDWNILGKTQGHGNSDLTPQGIEQAKELSEDI
                     GKYSIDYIFSSDLGRAMQTAQILADKLNIEVQKTEALREMGFGVWEGLLIKEIQKDYS
                     DIYATWRNEPHLVNIPEGETLKIIKERVDAFIKELNEKYDNKNIILVTHSITLRVMLL
                     SFLESGMENIYRIKQDNTALNIVEFKDYGPVIIKMNDTSHIKNHVKINNSALE"
     gene            1828..3081
                     /locus_tag="L3M60_002398"
     CDS             1828..3081
                     /locus_tag="L3M60_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN6227053.1"
                     /translation="MSKVIVVGGGASGMMAALSASKNNNEVILVERNGELGRKLRATG
                     GGRCNFTNNRDIEDFFDKVVSNKKFLYSSFYTFTNKDLISYFESRNLEYKIEEENDHK
                     VYTKNDKSIEVIEALNKDLLNHNVKIIYNKKVIDIITEEIAIKDDSNKNKSKYLIKGI
                     ILDNGDKILGDKIIISTGGVSYSKTGSDGLMYKILKNHGHTLNKLYPALVPLTIEEKW
                     IKNLQGISMKNVEISCKIKKRKISKSGDMLFAHFGITGPCVLIMSSYINKIIEKEKVE
                     LNIDFLPNLSSDEISSIIREFPNKNVLNNLKQILPQNFLKEILSLLSLSDKKASDLSK
                     ADEIKIIEYIKNMKLTCTGTTGINTGMVTSGGISVKEINSSTMESKLVKNLFFTGEVI
                     DIDAETGGYNLQIAFSTGYLAGISV"
     gene            3127..3777
                     /locus_tag="L3M60_002399"
     CDS             3127..3777
                     /locus_tag="L3M60_002399"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBN6227054.1"
                     /translation="MGNLVIAVDGPAGAGKSTIAKIVAKKLNINYIDTGAMYRAVTYK
                     CLKSGVDVNNEKEVIQIAENSDIDFKDNSIYLDKEVINEEIRTIEVSNNVSNVAKIKE
                     VRQLMVQVQRKIGMKSSVILDGRDIGSYVFPNADYKFFLVATPEERGNRRYKELCDKG
                     YNTTLKAVIEDIIRRDEIDSNREFAPLVKANDALEIDTTGKTIEEVVEEVISKINL"
     gene            3828..4424
                     /locus_tag="L3M60_002400"
     CDS             3828..4424
                     /locus_tag="L3M60_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HBN6227055.1"
                     /translation="MNFYKFVINIFKGFTRIFFKYEVIGAENIPDRGNIVIASNHKSN
                     LDPIFLAAAVENREIAAIAKKELFKVKPLGFILKKMHVMPINREKPDVSTIKTILRSV
                     RDGYVLGIFPEGTRIKGDSFGKAKAGLSVFTIKSKSKVVPVSIISKYKLFSKVIVYIG
                     EPISFEEHFKEKLTNDDHERISQEILEVIKQNYFKYSK"
     gene            4437..5276
                     /locus_tag="L3M60_002401"
     CDS             4437..5276
                     /locus_tag="L3M60_002401"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBN6227056.1"
                     /translation="MNIKIAKNAGFCFGVKRAMKMAWDEVEKSDSGIYALGPLIHNKQ
                     AVAKYEEKGLKTVNEIDTIPNHENMIIRSHGVPENIYKEAKNKKLKIVDTTCPFVKKI
                     HTVVSEYHNKGYEIIVIGDMKHPEVIGINGWCENSAIIIKTLEQMENMEFDNSKKYCL
                     VAQTTINPELYISIVNKLSDKLEEIVFNDTICSATKTRQESAKELAKEVDCMIVIGGK
                     HSSNTQKLVNVCEELVPTFAIETKDELDVNMLKKYKNLGITAGASTPNWIIEEVVTFL
                     ENL"
     gene            5529..6683
                     /locus_tag="L3M60_002402"
     CDS             5529..6683
                     /locus_tag="L3M60_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic efflux protein"
                     /protein_id="HBN6227057.1"
                     /translation="MEMLISASEEAFLHVGSMIGFFILLFGYINYKTSGNFTNIISKN
                     RKFQPLIGALIGAIPGCGGSLAIMPLYINGKLSFGAIIASLIASMGDAAFVLISSNIK
                     MYFFVTIVSTITGIITGQLVDYFKLEEKLGLKNRRKSDKYDTSKNNSKENKEKHNHDE
                     IILDTLAKSHGNTNRLAFIITHGKGYKIYIGIILIGFIFMSLAHSGLNLPIIEKLHSL
                     EEVIAVIGILFSIIYMWCFKKVFKNSNQKEEENKKISLREMLIHSVGEISFVITWIFI
                     AYLIYDLIILILGGDEYLVKLVLSTGVISVFIGAGLGLIPGCGIQIVLMSFYLKGTIP
                     LGAVIANSISQDGDALFPLLAMDKKSSLWAMVITTIPAIIVGMIVYAFLG"
     gene            6982..8679
                     /gene="ade"
                     /locus_tag="L3M60_002403"
     CDS             6982..8679
                     /gene="ade"
                     /locus_tag="L3M60_002403"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBN6227058.1"
                     /translation="MGNLKKQCDVASGREKADIVLKNGTIINVFTEELIVGDVAIVGD
                     TIVGIGDYKGTVEIDCSNKYISPGFIDAHMHIESTMVMPIELSKKLLKSGTTTIIADP
                     HELVNVKGASAIDFLLESTKDIPLNVYIMIPSSVPATSFETNGIGEFSAKDMESYINN
                     PRILGLGEVMCFNDVINSDNNILDKLELFKNKVVDGHAPNINGKSLQTYVCAGIENDH
                     ECITFDEVYEKLRAGLKILIREGSAAKNLKSIVNGMLKHNLPIEEFMFCTDDKHLDDI
                     EKQGHIRWNIKCAIDLGMEPIRAIKVATYNSARAYGLKRIGAIGAGYKADIVVLNDLE
                     KMEVDSVYKDGNLVKEEMFSNYNYDIKDKELLNTVKFKYINKEKIQLKVSEKNYVMEI
                     VPYQILTNKLYESLPCVEGYFIPSEEYSKLCVVERHRMTGNIAIAPLKGFGIKNGAIA
                     TTVAHDSHNIIVAGDNDDDIIVAINYLKEIQGGYVIVSNGKVLAHLSLQVAGLISTLT
                     AKEVQETTDNMLEIARKMGVPKYVDPFITLSFMALPVVPQIRLTDLGLFDVEEFKFI"
     gene            8921..9379
                     /gene="bcp"
                     /locus_tag="L3M60_002404"
     CDS             8921..9379
                     /gene="bcp"
                     /locus_tag="L3M60_002404"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBN6227059.1"
                     /translation="MLSIGTRAPEFTLEDKDGNKVSMSDFKGKKVVVYFYPKDNTPGC
                     TRQACAFRNAYDGFKKEDVQVIGISKDSIKSHQKFAEKHELPFILLSDPDLVAIKAFD
                     VWKEKKMYGKTALGVVRATYIIDENGIIEKVFEKAKPDTNAQEILEYLEK"
     gene            9395..10138
                     /gene="yaaA"
                     /locus_tag="L3M60_002405"
     CDS             9395..10138
                     /gene="yaaA"
                     /locus_tag="L3M60_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBN6227060.1"
                     /translation="MIIMLSPAKNMKNIEVSDRDLSLPCFIDNTKEIVENINTFSVED
                     FKKKMKINEKLAILNKNRFESIKFDKLGSPAILTYDGIQYKNIEAENFTRQDEEFANS
                     CIRIISGLYGVVKPYDSIYEYRLEMQAKLRVGEFKNLYEYWGNRIYKELIKEKTAIVN
                     LSSNEYSKSIEKFIKDSDTYITCTFKVNKNGIFKVESTQAKKARGMMTKYIIKNRIRD
                     IEELKKFNLEGYKYQENLSNNSEYIFVKE"
     gene            10266..12926
                     /locus_tag="L3M60_002406"
     CDS             10266..12926
                     /locus_tag="L3M60_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HBN6227061.1"
                     /translation="MENHKQMPWHSRATHEILESLDSSEEGLTDTEAEKRLKENGLNE
                     LRKKPNRTTLQMLWSQITDAMVLILIGAAILSLIFGEFTEAFVILIIVTVNAVIGIVQ
                     EKKAESSLEALKNMNSPTARVMRDGEENVIPASNLVVGDIVFLEDGAMVPADLRLIET
                     SSLKIQEASLTGESVPSEKDADTILPKEYVLGDRSNMAYTSSIVTYGRGVGVVVATGM
                     NTEVGNIAHLLDSQDDFDTPIKRKLNTVGKTLSVIGIIVCVVIFAIGAFYQRPLLPMF
                     MIAISLAISIIPEGLPATATIVMALGVQRMSKRNALIRKLPAVETLGGATVICCDKTG
                     TLTLNKMTVTHIAVNGDFESGKVTSIDTASEKHPLVYKEIVYAGALCNDACINPDKTD
                     EILGDPTEGALIFMAKKFGIDQEILEEEYPRVFEQPFDSDRKCMTTVHNIDEKIIAYT
                     KGAVDEILDLCTKILTSKGERNITELDKKNIHELCLNMSKDALRVLGFAKREISSIPK
                     EDSENIEYDLTFIGIVGMIDPPRTEVIDAVSTCKEAGIRTIMITGDHKVTATTIAHEL
                     GIWSEENTVISGDELDNLSDDELDEAVKNTTVFARVSPFDKLRIIQSLKRIGEVPAMT
                     GDGVNDSPALKSADIGIAMGISGTDVAKDSSDMILMDDSFTTISYAIKEGRRVYRNIQ
                     KVIQFLLVGNIAEILTLFVAILLNWDTPLLAVHILWVNLATATLPALALGVDPPSRNI
                     MKHQPVKSTTLFEKNLIQRVVTQGIFVAIMTILAYKIGLNIENHSVGQTMAFCVLAFS
                     QMLRAFSQRSNTDSMFNRNNGKNPFLLISFLTSALLIAVILFVPVFRNAFNLTILNSL
                     EWLITIALAIMSVVQVEVGKIIKRKKLKNS"
     regulatory      12976..13078
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            13186..14451
                     /locus_tag="L3M60_002407"
     CDS             13186..14451
                     /locus_tag="L3M60_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HBN6227062.1"
                     /translation="MYNKETICVQGNYKPGNGEPRVLPLYQSTTFKYSSIDQLAELFD
                     LKVDGHIYSRISNPTIQAFEEKISLLEGGVASVAVSSGQSANMLAVLNICKSGDSILC
                     SSKVYGGTFNLLGPSLKKFGIDLISFDLDSSEDEIVELAKENTKVVFAETLANPTLEV
                     IDFEKIANVAKRINVPFIVDNSLASPVLCNPLKYGANIVTHSTTKYLDGHASSVGGII
                     VDGGNFNWDNGKFPELVEPDPTYHGISYTQKFGNAAYATKARVQLLRDYGNCLSPFNA
                     YLTNLNVETLHLRMERHSENAFKIAKFLEKHENVDWINYPGLEDNKYYENAKKYLSKG
                     CSGVLSFGVRGGLENAKKFVEKLQIASLVTHVSDVRTCVIHPASTTHRQLTEEQLIAS
                     GVLPSLIRLSVGIENVEDLIADLNQALNF"
     gene            14481..>14679
                     /locus_tag="L3M60_002408"
     CDS             14481..>14679
                     /locus_tag="L3M60_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBN6227063.1"
                     /translation="MALILPKGLPVINKLLDEGIDVIYKEDFKKKLTYEENIDTKIAI
                     LNLMPIKIDTELDLLRRIDKTG"
BASE COUNT         5909 a         1558 c         2640 g         4572 t
ORIGIN      
        1 gattatattg ataaaaaaca ctaaaattaa attaataaag tacttggagg gatggaaatg
       61 aaagaggaaa attttattaa tattgaatac gaggataaac gtttttcaat taatagtaaa
      121 gaagttttgt acatagaaaa gcaagaatat gaagtaaaga tttacacaga aaatggtaat
      181 tatgaagttg gagaaaatgc tccttgtata agaaaactaa tgaaagaaga aaattttttc
      241 aaatgtagta atagttatat tgtaaattta gataagtgtg aattttatct cagcaataat
      301 tgtatttttg tgaataataa ttttataaga attttaggtt cagaaagaaa aaagaagtta
      361 aaaaagaaat tagaaaaatt atatgattat gaagattaat attgagaggc tgtattttat
      421 acagcctctt tatttattca ttgtattatt aataaatttt aaattatgat aaaatataaa
      481 acaagtaact tatttcagaa aaaaatactt tttaaaagaa agggacgaga tatgggagat
      541 tataataaaa tgcttgaatt aaagaataat tcaggtacta gtgaaagaaa attaaaatat
      601 ccttgtatct ataaacattt caaagggaaa tattatgcta caatgggtct tagcaaagct
      661 attgataatg tagaaaacat atgtgaaata tatagtaaag aaaatttaat ccaaaataga
      721 aataaatata aattagttat tagacatact gaaagagaag aagatatcta tgtctataga
      781 gatttagatg gaaactttta tcataaaaaa gaagaagata ccaatgattt agttttatac
      841 aagactttat atgacgatac aggaatattt ggaagacctt tggatatgtt tttagaaaaa
      901 gtagatacag ataagtatgt caattcaatt caagagtaca gatttgaaga agtatataaa
      961 taatataaga gaaatatgtt tataaattta gaaaaattat ttaagagtat aataatttgc
     1021 agaaatacaa atgagattta ttgtaaacaa tatgttagat tggattataa aatagaaaca
     1081 aagtaaagta ttatatgata taattaagtt ttgagaagtg atattatatt taataaaaaa
     1141 taggcatata ttaggaggac atatgggaaa tactttttat atagtaagac atggacaaac
     1201 agattggaat atattaggaa aaacacaagg gcatggtaat tctgacctta cacctcaagg
     1261 tatagaacag gcaaaagaac tttctgaaga tattggcaag tattcaattg attatatttt
     1321 tagtagtgac cttggaagag caatgcaaac agctcaaatt ttagcagata agctcaatat
     1381 tgaagttcaa aaaactgaag cacttagaga aatgggattc ggagtttggg aaggtctttt
     1441 aataaaagaa attcaaaagg attattcaga tatatatgca acatggagaa atgagccaca
     1501 tttagtaaat ataccagaag gcgaaacgct taaaattata aaagaaagag tagatgcttt
     1561 tataaaggaa ttaaatgaaa agtacgataa taaaaatata attcttgtta cacattcaat
     1621 tacattgagg gttatgttgt tgtcattttt agaatcaggg atggaaaata tatatagaat
     1681 taagcaggat aacactgctt taaacatagt agaatttaaa gattacggac ctgttataat
     1741 aaaaatgaat gatacaagtc atataaaaaa tcatgtaaaa ataaataatt ctgcattgga
     1801 gtaggaatca attataagga gaaatttatg agtaaagtta tagtagttgg tggaggagct
     1861 tctggaatga tggcagcttt gtcagcttca aaaaataata atgaagtaat attagttgag
     1921 agaaatggag aattaggaag aaaactgaga gcaactggtg gaggtagatg caattttacc
     1981 aataacagag atatagaaga tttttttgat aaagtcgtta gcaataaaaa atttctctat
     2041 agtagtttct atacatttac aaataaagac cttatttcat attttgaaag tagaaatttg
     2101 gaatataaga ttgaagaaga aaatgaccac aaagtatata ctaaaaatga taaatctata
     2161 gaggtaatag aagcattaaa taaagattta ctcaatcaca atgtaaaaat aatatataat
     2221 aaaaaagtta tagatattat aactgaagaa attgctataa aagatgattc taataagaac
     2281 aagtcaaagt atctaataaa agggattatt ttagataatg gagacaagat actaggagac
     2341 aagattatta tatcaactgg aggagtgagt tattcaaaaa cgggttcaga tggtttaatg
     2401 tacaagattc ttaaaaatca tggacataca ttaaataaac tatatcctgc tttagttcca
     2461 cttacaatag aggaaaaatg gattaaaaat ttgcaaggaa tatccatgaa gaatgtggaa
     2521 atatcttgta agattaagaa aaggaaaata tcaaaatcgg gagacatgtt atttgcacat
     2581 tttggaataa caggaccttg tgtgcttata atgtcttctt atattaataa gattattgaa
     2641 aaagaaaagg tagaattaaa tatagatttt ttaccaaatt taagtagtga tgaaatttct
     2701 agtataatca gagaatttcc aaataaaaat gttctaaata atcttaagca aatactacct
     2761 caaaatttct taaaagaaat tctatctcta ttatctttga gtgataaaaa ggcaagtgat
     2821 ttgtctaagg cagatgaaat taaaattatt gagtatataa agaatatgaa gctaacttgc
     2881 actggaacta caggtataaa cactggtatg gttacttctg gtggtatttc agttaaagaa
     2941 attaattctt ctactatgga atctaaatta gttaagaact tattttttac aggggaagta
     3001 attgatatag atgcagaaac tggtggatac aatttacaaa tagcattttc aactggatat
     3061 cttgcgggta ttagtgtata aatataattt tttaataatt aaatatgaat aaatagaggt
     3121 gtatatatgg gtaatttagt tatagctgta gatggtccag caggagctgg aaaaagtaca
     3181 atagccaaga tagtagcaaa aaagctaaat ataaattata tagatacagg tgctatgtac
     3241 agagcagtta cttataagtg tttaaaaagt ggggttgatg tcaataatga aaaagaagtt
     3301 attcaaatag ctgaaaattc ggatatagat ttcaaagata atagtatata tttggacaaa
     3361 gaagttataa atgaagaaat aagaacgata gaagtaagta ataatgtttc taatgtggca
     3421 aaaataaaag aagtaagaca gttgatggtt caagttcaac gaaaaatagg aatgaaaagt
     3481 tcagtaatac tagatggtag agatataggt tcatatgttt tcccaaatgc agattacaaa
     3541 ttctttttag tagctactcc agaagaaaga ggaaatagac gctataaaga gctttgtgat
     3601 aaaggatata atactacttt aaaagcagta attgaagata taataagacg agatgaaatt
     3661 gattcaaata gagaatttgc accattagta aaagctaatg atgcactaga aatagatact
     3721 acaggaaaaa ccatagaaga agttgtagaa gaagttatat ctaaaataaa tctttagttt
     3781 tttatgtatt ataataaaaa taaatgtagt gttgaaggga gataaacgtg aatttttata
     3841 aatttgtaat aaatattttt aaaggtttta ctagaatatt ttttaaatat gaagttatag
     3901 gtgcagaaaa tattccagat agagggaata tagtaatagc atcaaaccat aaatcgaatc
     3961 tggaccctat attcttagct gctgccgtag aaaatagaga aatagctgca attgcaaaga
     4021 aagaattgtt taaagtaaaa ccattaggat ttatattaaa aaaaatgcat gttatgccta
     4081 taaatagaga aaagccagat gtatctacaa taaaaactat tttaaggtca gttagagatg
     4141 gttatgtgtt aggcatattt ccagagggta ctagaataaa gggagattct tttggtaagg
     4201 ctaaggcagg tctttctgta tttacaataa aaagtaaatc taaagttgtt ccagtatcaa
     4261 taatatccaa atacaaactt tttagtaaag taattgtata tattggagaa ccaatatcat
     4321 ttgaagaaca ttttaaggaa aaattgacta atgatgacca tgaaaggata tcacaggaga
     4381 ttttagaggt cataaaacaa aattacttta aatattcaaa ataagggggt aaatttatga
     4441 acataaaaat agcaaaaaat gctggatttt gttttggagt aaaaagagca atgaaaatgg
     4501 catgggatga agttgaaaaa agtgattctg gcatatatgc tttaggtcct cttattcata
     4561 acaagcaagc agtagcaaaa tatgaagaaa aaggtcttaa gactgtgaat gaaattgata
     4621 caataccaaa tcatgaaaat atgataataa ggtctcatgg agttcctgaa aatatatata
     4681 aagaagctaa gaacaaaaaa ttaaaaattg tggacactac atgtccattt gtaaagaaaa
     4741 tacatacagt agtaagtgaa tatcataata aaggctatga gataatagtt attggagata
     4801 tgaaacatcc agaagttata ggtataaatg gatggtgtga aaattcagct ataataataa
     4861 agactttaga acaaatggaa aatatggaat ttgataactc aaaaaaatat tgccttgttg
     4921 cacagactac aataaatcca gagttgtata taagtatagt taataaacta tcagataaat
     4981 tagaagaaat agtatttaat gatacaattt gttcagctac aaaaacaaga caagagtcag
     5041 ctaaggagtt agctaaagaa gttgattgta tgatagttat aggaggcaaa cacagctcaa
     5101 atactcagaa gttagtaaat gtttgtgaag agttagtacc aacttttgca atagagacta
     5161 aggatgaatt ggatgtaaat atgcttaaaa aatataaaaa tttaggtatt acagcaggag
     5221 catctacacc taattggata atagaagaag ttgtaacatt ccttgaaaat ttataaattt
     5281 gatagataat aaaaataggt ggatattatt ataacataaa acttattata atatctgcct
     5341 ttgatttcat attacatgtg gcaaatagat aacctaatat taaaaagtgt gtatcttaag
     5401 atgaaaaact aatagtataa gatgcgcact ttttaatatt agaaatttaa aataaatatt
     5461 gatattgatt atcaatagaa aatggaatat aatataatta ttgcaaaatt agtctaggag
     5521 gtaaattaat ggaaatgtta atatccgctt ctgaagaggc gtttttacat gtaggttcta
     5581 tgattggatt ttttattttg ttatttggat atattaatta taaaactagt ggaaatttta
     5641 ctaatattat atcaaaaaac agaaaatttc aacctttaat aggtgcttta attggagcta
     5701 taccaggttg tgggggaagt ttggcaataa tgccattgta tataaatgga aaattaagct
     5761 ttggagcaat aattgcaagt ttaatagcta gtatgggaga cgctgcattt gtacttattt
     5821 catcaaatat taaaatgtat ttttttgtca ctatagttag tactattaca ggaataataa
     5881 ctggacaatt agtggattac tttaaactag aagaaaaatt aggtctaaaa aaccgtagaa
     5941 aatcggataa atacgataca agtaaaaata actctaaaga aaataaagaa aaacataatc
     6001 atgatgaaat aattttggat actctggcta aatctcatgg aaatacaaat agacttgcat
     6061 tcataataac acatggaaag ggatataaaa tttatatagg aattatatta atcggtttta
     6121 tatttatgtc acttgctcac tctggactta atcttcctat aattgagaag ttacattcat
     6181 tggaagaagt aatagcagtg attggaatat tattttcaat aatttatatg tggtgtttta
     6241 aaaaggtatt taaaaattct aatcagaaag aggaagaaaa taaaaagatt tctttacgag
     6301 aaatgctgat acattctgtt ggcgaaatat catttgtaat tacatggatt tttattgcat
     6361 accttattta cgatttgata atattaattt taggtggaga tgaatattta gttaaactag
     6421 tattatcaac aggtgtaata agtgtattta taggtgcagg tcttgggctt attccaggtt
     6481 gtggtataca gattgtactt atgtcatttt atttgaaggg aactatacca cttggggcag
     6541 tgatagccaa ctctatttct caagatggag atgcactttt cccactttta gcaatggata
     6601 aaaaatcttc tttatgggct atggttatta ctactatacc agcaataatt gtaggaatga
     6661 tagtttatgc atttttaggt taaatataaa taaaaaagta ttaaaaaatt aataaatcac
     6721 ataaatttat ttgcaagaat agtaaactta aatatataaa taaaaaattc aatataactt
     6781 aaattattac ttaaatatta atgagaacat gggagataat attattcctt atgttctctc
     6841 tatttaatta aatgaattat ggaacaaatt tcaagtgata ctttttatta aaactcatat
     6901 ataatatata ttaataaatt aaactataat atgtaataca gccagtatat tgatataaaa
     6961 aacttaagag gagttgtaaa aatgggtaat ttaaaaaaac agtgtgatgt tgcaagtgga
     7021 agagaaaaag ctgacattgt acttaaaaat gggacaatca taaacgtatt tacagaagag
     7081 ctaatagtag gagatgtagc aatagtagga gatactattg ttggtattgg cgattacaaa
     7141 ggtactgtag aaattgattg tagcaacaaa tatatatcac ctggttttat tgatgcacat
     7201 atgcatattg aatctacaat ggtaatgcca attgaattat caaaaaaatt attaaaatca
     7261 ggtacgacaa ccatcatagc cgacccacat gagttggtaa atgtaaaagg agctagtgca
     7321 atagattttt tattagaatc gacaaaagat ataccactta atgtatatat tatgatacct
     7381 tcatccgtac ctgcaacaag ttttgaaact aatggaattg gtgaatttag tgcaaaagac
     7441 atggagagtt acataaataa tcctagaatt ttaggcttag gagaggtaat gtgctttaat
     7501 gatgttataa attctgataa taatatcttg gataaactag agctttttaa aaataaagtt
     7561 gtagacggac atgcacctaa tataaatggt aaatcgcttc agacatatgt atgcgcagga
     7621 atagaaaatg accatgaatg tataactttt gatgaagtat atgaaaaatt gagagcaggt
     7681 ttaaaaatat taataagaga aggaagtgca gcaaaaaatt taaaatctat agtaaatggt
     7741 atgctaaagc ataacttgcc aattgaagag tttatgtttt gcacagatga taagcactta
     7801 gatgatatag aaaaacaagg tcacataaga tggaatataa aatgtgcgat tgatttaggt
     7861 atggaaccta ttagagctat aaaagttgca acttataatt ctgcaagagc atatgggcta
     7921 aaaagaattg gagcaatagg tgctggttat aaggcagata ttgttgtatt gaatgattta
     7981 gaaaaaatgg aagtggatag tgtatataaa gatggtaatt tggtaaagga agagatgttt
     8041 tctaattaca attatgacat taaagataaa gaattgctta atacagtcaa atttaaatat
     8101 attaataagg aaaaaataca gttaaaagta agtgaaaaaa attatgtaat ggagattgta
     8161 ccttatcaaa ttttgacaaa taagttatat gaatcattac catgtgtaga gggatacttt
     8221 attccaagtg aagagtattc aaaactttgt gttgttgaga gacatagaat gactggaaat
     8281 atagcaattg ctccactaaa aggatttggc ataaaaaatg gagcaattgc tactactgtg
     8341 gctcatgatt ctcataatat tatagttgca ggggataacg atgatgacat tatagttgcc
     8401 ataaattact taaaggaaat acagggtggt tatgtaattg tatcaaatgg aaaagttctt
     8461 gcacacctaa gtcttcaagt ggctggatta attagtactc ttacagccaa agaagtacaa
     8521 gaaactactg ataatatgtt ggagatagca agaaaaatgg gagttccaaa gtacgtagac
     8581 ccttttatta cactttcttt tatggcactt ccagttgttc cacaaataag acttacagat
     8641 ttaggtctat ttgatgttga ggaatttaaa tttatatagg caccataata ataaattagg
     8701 atattcaaaa atagagattg tactacaccc caattgttgg actaaaaacc taatgattgg
     8761 ggtgtttata tataactaaa tacaatgcat ataaatacat gaaaattata tatttatgaa
     8821 actttataca taaaaaataa aatattttat ttatatatag atttataggg taataatata
     8881 agcatagtat agaattatca ataaaaaagg agagatattt atgttgagta taggaacaag
     8941 agcaccagaa tttacactag aagacaaaga tggaaataag gtaagtatgt cagattttaa
     9001 aggtaaaaaa gtagtagtat atttttatcc taaagataat acaccaggat gtacacgtca
     9061 agcttgtgct tttagaaatg cgtatgatgg atttaaaaag gaggatgtac aagttattgg
     9121 tataagtaaa gatagtataa aatcacatca aaaatttgca gaaaagcatg aacttccttt
     9181 tattttactt tctgaccctg atttagtcgc tataaaagcc tttgatgtat ggaaagagaa
     9241 aaaaatgtat ggaaaaactg cattgggagt tgtacgtgcg acatatatca tagatgaaaa
     9301 tggaattatt gaaaaagttt ttgaaaaagc aaaaccagac acaaatgcac aagagattct
     9361 tgaatatttg gaaaaatagg agcaataaga atttatgatt ataatgttat caccagctaa
     9421 aaatatgaag aatatagaag tgtctgatag agatttgagt ttgccctgtt ttatagataa
     9481 tacaaaagaa atagtggaaa atataaatac atttagtgta gaagatttta aaaagaaaat
     9541 gaaaattaat gaaaaattag ctatactaaa taaaaataga tttgaaagta ttaagttcga
     9601 taaattagga agtcctgcaa tcttaactta tgatggaata caatataaga atatagaagc
     9661 agaaaatttt acaaggcaag atgaagagtt tgcaaatagt tgtattcgaa tcataagtgg
     9721 attgtatgga gttgtaaaac catatgatag tatatatgaa tatagattag aaatgcaggc
     9781 aaaattacga gtaggtgagt ttaagaattt atacgaatat tggggaaatc gtatatataa
     9841 agaactaatt aaagaaaaaa cagctattgt aaatctttca tcaaatgaat acagcaagtc
     9901 catagaaaaa tttattaaag atagtgatac atatattaca tgtactttta aagtaaataa
     9961 aaatggtata tttaaagtgg aatctacaca agcaaagaaa gctagaggta tgatgacaaa
    10021 gtacattata aaaaatcgga taagagacat tgaagaactg aagaagttta atttagaagg
    10081 gtataagtat caagaaaact tatctaacaa tagtgaatac atatttgtaa aagagtagaa
    10141 attttattaa atgttaaatg acattgttaa atttaggtaa aatatactta gtgtgtattg
    10201 aattgaactt gattttaaaa tataattatt cttaaatcaa aaaaagaaag gagtggtctt
    10261 ccaaaatgga aaatcacaaa cagatgcctt ggcattcaag agcaactcat gaaattttag
    10321 aaagtctaga ttcatcagag gaggggctca ccgataccga ggcggaaaaa cggcttaaag
    10381 agaatggttt aaatgaactt agaaaaaaac caaatagaac aactttgcaa atgctttgga
    10441 gtcaaattac agatgctatg gttctgatac taataggagc agcaatattg tccttgatat
    10501 ttggagaatt tacagaagct tttgtaatac taataattgt aacagtaaat gcagttattg
    10561 gaattgttca agagaaaaag gcagaatcat cattagaagc attaaaaaat atgaactcac
    10621 caacagctcg tgttatgcgt gatggtgaag aaaatgttat tccagcaagt aatcttgtag
    10681 taggagatat tgttttttta gaagatggag cgatggttcc agcagacctt cgtttgatag
    10741 aaacatctag tttaaaaatt caagaggctt ctttaacagg agaatctgtt ccatcagaaa
    10801 aagatgcaga tacaatctta cctaaagaat atgtattagg agaccgtagc aatatggcgt
    10861 atacatcttc aattgtcaca tatggtagag gtgtaggtgt tgtagtagca actggtatga
    10921 atacagaagt gggtaacata gcacatcttt tagattcaca agatgatttt gatactccta
    10981 taaaacgtaa acttaatact gttggaaaga cattaagtgt aataggtatt attgtatgcg
    11041 ttgttatatt tgcaataggt gcattttatc agagacctct cttgccaatg tttatgattg
    11101 caatttccct tgccatatca attattccag agggattacc tgcaacagct actattgtaa
    11161 tggctttagg cgtacaacgt atgtctaaaa gaaatgcttt aattagaaaa ttacctgctg
    11221 ttgaaacatt aggaggagct acagtaatat gttgtgataa aactggtaca ttaaccttaa
    11281 ataaaatgac tgttacacat attgctgtca atggagattt tgaatctggg aaagtaacat
    11341 caatagatac tgcatctgaa aaacatccgt tagtgtataa agagattgtt tatgctggtg
    11401 cactttgtaa tgatgcatgc attaatccag ataaaactga tgaaatatta ggtgacccaa
    11461 cagagggtgc attaattttt atggccaaaa aatttggaat agatcaggaa attcttgaag
    11521 aagaatatcc acgtgtattt gaacagcctt ttgattctga tagaaaatgt atgacaactg
    11581 ttcataatat agatgaaaag attattgctt atactaaagg agcagtagac gaaattcttg
    11641 acctatgtac taaaatattg acttcaaaag gtgaaagaaa cattaccgaa ttggataaaa
    11701 agaatataca tgagttatgt cttaacatgt ctaaagatgc attgcgtgta cttggttttg
    11761 ctaagcgtga gatttcatct ataccgaaag aagatagtga aaacatagaa tatgatttga
    11821 catttatagg tatagttggt atgattgacc caccaagaac tgaggtaatt gatgcagtaa
    11881 gtacttgtaa agaagcgggt ataagaacca taatgataac aggtgaccat aaagttactg
    11941 ccacaacaat agctcatgag ttaggaatat ggagtgaaga aaatactgtg atatctggtg
    12001 atgaacttga taatttgagt gatgatgaac tagatgaagc tgttaaaaat actacagtat
    12061 ttgctcgtgt atccccattt gataaactta gaattataca atctttaaaa agaattggag
    12121 aagtaccagc aatgactggg gatggtgtaa acgattctcc tgccttaaag tctgctgata
    12181 ttggtatagc tatgggtata agtggtacag atgttgcaaa agattcttct gacatgattt
    12241 taatggatga tagctttacg acaatttctt atgcaataaa agaaggtaga cgtgtatata
    12301 gaaacataca aaaagttata cagttcttgc ttgttggtaa tatagcagag atacttacat
    12361 tgtttgtagc aatattatta aattgggata ctccacttct tgctgttcat attctgtggg
    12421 taaaccttgc aactgctact cttccagccc ttgcgcttgg agttgatcca ccaagcagaa
    12481 atataatgaa gcatcagcca gtgaaatcaa ccactttatt tgaaaaaaat cttatacaaa
    12541 gagttgttac tcaaggtata tttgtggcaa taatgactat tttagcatat aaaattggtc
    12601 ttaatataga aaatcattct gtaggtcaaa caatggcatt ttgtgtatta gcattttcac
    12661 agatgttacg agcatttagt cagcgttcaa atacagattc aatgtttaat agaaacaatg
    12721 gtaaaaatcc atttctttta atttcatttt taacttctgc attgctaata gcagtgatac
    12781 tgtttgtacc tgtttttaga aatgctttta acttaacaat tttaaatagc ttagaatggc
    12841 ttataacgat tgctttggct ataatgtctg tagttcaagt agaggttggg aaaattatta
    12901 aaagaaaaaa attaaaaaat agttgacaaa attaggaaaa atatataaga tataaaacat
    12961 agaaaaatta aataaatctt atccagagag gtggagggac tggccctatg aaacccggca
    13021 accagtatat ttatatacaa tggtgctaaa tcctgcggcg aaagctgaga gatgagtata
    13081 attaagctta tacagagcct gagttatata ctcaggcttt atttatttta actctcatct
    13141 gataaatagt ttaatttttt actaaaatct aagggggata caagaatgta taataaagaa
    13201 acaatatgtg tgcaaggaaa ttataaacca ggtaatggag aaccaagagt attaccttta
    13261 tatcaaagta caacttttaa atatagcagt atagaccaac ttgctgaatt atttgactta
    13321 aaagttgatg gacatatata ttcaagaata agcaatccta ctattcaagc ttttgaagaa
    13381 aaaataagtt tactagaggg aggagtagct tctgtagctg tatcatcagg tcaatctgca
    13441 aatatgttag cagttttaaa tatatgtaaa tcaggagata gtatactttg ttcttcgaaa
    13501 gtatatggag gaacattcaa tttattagga cctagtctta aaaaatttgg tatagattta
    13561 atatcatttg acttagattc aagtgaagat gagatagtag aacttgcaaa ggaaaatact
    13621 aaggttgtgt ttgcagaaac acttgcaaat ccaactcttg aagttataga ttttgaaaaa
    13681 atagcaaatg tagctaaaag aattaatgtt ccatttattg ttgataattc attagcatct
    13741 ccagtgcttt gtaatccttt aaagtatgga gcaaatatag ttactcattc tactacaaaa
    13801 tatttagatg ggcatgcttc aagcgttgga ggaattatag tggatggtgg aaactttaac
    13861 tgggataatg gaaaatttcc agaattagtt gaaccagacc caacgtatca tggaataagc
    13921 tatactcaaa aatttggaaa tgctgcatat gcaactaaag caagagttca gttgcttaga
    13981 gactatggaa attgtttaag cccattcaat gcatatctta ctaatttaaa tgttgaaaca
    14041 ctacatctta gaatggagag acatagtgaa aatgcattta aaatagctaa atttttagaa
    14101 aaacatgaaa atgtagattg gattaattac ccaggacttg aagataacaa gtattatgag
    14161 aatgccaaaa agtatttatc aaaaggatgt agcggagtct tatcatttgg agtaagaggt
    14221 ggattagaaa atgctaaaaa atttgtggaa aaactacaga tagcatcttt ggttacacat
    14281 gtttcagatg taagaacttg tgttatacat ccagcttcaa ctactcatag acaattaaca
    14341 gaagaacaat taattgcatc tggagtattg ccttcgctaa taagattgtc tgttggaata
    14401 gaaaatgtgg aagatttaat agctgattta aatcaagctt taaattttta atattctaag
    14461 agaaagagaa gtgaaaagat atggctttaa tactaccaaa gggattacct gttattaata
    14521 aattattaga tgaaggaata gatgtaattt acaaagaaga ttttaaaaag aaattaacat
    14581 atgaagaaaa catagatact aagatagcca tactgaatct aatgcctata aagatagata
    14641 cggaattaga tttattaaga aggatagata aaacaggat
//
LOCUS       DAFNNB010000096        14475 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0097, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000096 DAFNNB010000000
VERSION     DAFNNB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0097"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            86..649
                     /locus_tag="L3M60_002409"
     CDS             86..649
                     /locus_tag="L3M60_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227064.1"
                     /translation="MNKVYIVTTYTGTILSYLIRNISKKLYTHVSISLNENLKPMYSF
                     GRLNPRNPFIGGFVEENINQGLYAIRKNTVCRVYSLEVDNLQYENLYKNIKSISDYRE
                     DYYYDTMALIYLPMNIHRHSEYKYVCSNFVADMLEKSEIDILNKQAFEVTPNDFYNLS
                     GLTLEYEGLLSEYNSRQHSYVSKIANI"
     gene            954..2654
                     /locus_tag="L3M60_002410"
     CDS             954..2654
                     /locus_tag="L3M60_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227065.1"
                     /translation="MKRYLKTYGVALIIVIIYAFIKLPVLRLDFTSLFSVGIIFFVVA
                     GILDMMLDRNERASKMAKYNFSIAIALLVYIVVVPFITSTPVFHAKSYRELLGKVTES
                     KFTDDISPVSVNDIRLVDEDMAMKLGDKKLGEVPAIGSVSKLGKFHIQNVDGELYWVA
                     PLVHRDIIKWITSLSGTSGYVKVSASNPQDVQLVQEIDGKPIKIVYQPEAYLHQDLQR
                     HLYIHGIVNVGMTDFTLEINDEGRPYWVVSLYEHKIGYGGANATGIATVDAETGKINV
                     YDVKNTPKWVDRIQPQNFITDQIKDWGVYVNGFLNSVISEKGVLVPTEGTSLVYGNDN
                     RSYWYTGITSSGGDESTIGFMLVDSRTKEARLYKQPGATETAAMKSAEGNVQEKNYEA
                     TFPVMYNILGQPTYVSSLKDKAGLVKRVAFVSVEDYNVLGIGEDKNEALRNYKDALES
                     KGNDLKLDNELKDEVIEGTITRISPDVRGGNTNYYVTLDTNKGIIFRATSKVSSELPL
                     TQVGDKVKISYSKEESGVIEMSEFDNLNIANFEDKKAKEGTGTDENTDSQDSENQGNV
                     "
     gene            2824..3441
                     /locus_tag="L3M60_002411"
     CDS             2824..3441
                     /locus_tag="L3M60_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227066.1"
                     /translation="MTNMEKEILLLISRVRCVTESQFRKIYGDQKRYNKKNFKKTLRK
                     MCSEYTLKKYPCDVDYYNYKDLSYVYYLNGSKLFKGDDLIKALVGSELVVRINTAGCD
                     VKRFYRNVSVDDVKYDLFIEYTDQFGNIKQVLVDVDLNNEIDISKYENLSKKIDKSTI
                     PFFKVPNVLVVTPDNLSELNDLERYDNEYSINFIDLGLNKLFNCI"
     gene            3802..4995
                     /locus_tag="L3M60_002412"
     CDS             3802..4995
                     /locus_tag="L3M60_002412"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HBN6227067.1"
                     /translation="MSIIVQKYGGSSVADTEKIKSIAENIIERRKENPQMVIVVSAMG
                     KSTDEYITLAKELSNEPSKRELDALMSTGEMISASLLSIALNALGCKAISYNAYQLNI
                     KTSGLHGKSQIDDINVNRIKNSLDEGNVVIVTGFQGINEDGDVTTLGRGGSDTSAVAL
                     AVKLNGKCEIYTDVDGIYFTDPRKYSKASKLDEIEYEEMLELASLGAQVMHSRSIELA
                     QKYGVEIYVGRTCGTEKGTYIRGGKDMKLEDKVITGLATSDDDSSITIKDFKAENISS
                     LFEDIATIGISVDMISQTAPILDKISVSFTVPKEELGECKKIVSKYTDEEHVVIDNNI
                     TKFSLVGLGMKNTSGVAAKVFKIFNENGIMIKLITTSEIRITCAINSDDKQVAIEKIA
                     EVFNI"
     gene            5013..6305
                     /gene="lysA"
                     /locus_tag="L3M60_002413"
     CDS             5013..6305
                     /gene="lysA"
                     /locus_tag="L3M60_002413"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBN6227068.1"
                     /translation="MKLHGTMTVNNDELYIGGVSCLELSKKYQTPLYVFDEALVRQNC
                     REYIKYFRAREGKNKVAYAGKAFLPLYMCNLINEEQMCLDVVSGGELYTAYKSNFPME
                     KVFFHGNNKTKEEINMGLDLGVGKFVVDNFYELDLLEALCEDKGKTQEIYFRITPGIE
                     AHTHDYIKTGQIDSKFGFALVNGDLFRAIEKLSDYKNIKLVGLHAHIGSQIFDVEPYI
                     DTVDIMMNLVKEIKEKFNIHLTEIDLGGGVGVYYSEGDKPKTIKEFCETIITKAETSC
                     KELGIEVPTLVIEPGRSIVANAGSTLYTVGSIKDIKDVRVYVSVDGGMTDNVRPSLYQ
                     ASYECSIVNKINHKAMNHVTIAGKCCESGDILIGDINIMDIKSGDILITTSTGAYGYS
                     MSSNYNKIPRAAVVSVFDGNDKIICKRQSYEDLLALEV"
     gene            6502..7176
                     /locus_tag="L3M60_002414"
     CDS             6502..7176
                     /locus_tag="L3M60_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227069.1"
                     /translation="MTRRDLMRGRVILLISILSIFLVGCSFNKKDEINLVEQGKTYLE
                     KHNYKKAMESLSSALEEDSTNENARAMYMQAMRMSNMTEFEELKNYEGAIKELKLIEK
                     IKNGSSVIKEEATKKKEELTKLEKEQNEAEEQRKKKAKDTAGEDRYRVESDARKSSYS
                     GSSKNNKSSSKNNKKSDSDKSDNNNNSSQNQEKPNSSNPTPAPTPQDTSGGSDSSGNS
                     QNSNNQ"
     gene            complement(7330..8415)
                     /locus_tag="L3M60_002415"
     CDS             complement(7330..8415)
                     /locus_tag="L3M60_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBN6227070.1"
                     /translation="MIIISKENIKYYLIVVFIGILFFKFINTPSDFISSIEGFLKFFS
                     PFLLAILLALLLNPLVMLFEVKFKAHRLLAIFLSYIFIGFILAFAIRLLIPSIANTLN
                     RLINEMPMYTDYINSFIEKNMSNIDFLKTLIPHIQHSLDNVLKEASNFIGKISKNFLI
                     YTLSISSMLFNMTMGFILSIYIIYDKEKIALGFKRFLYSSTTRNKADSIIEFFRTTHD
                     IFYDYLLGRILDSLIIGIIAFLGFQFVIRIENALFLASIIFLGNIIPYFGPFIGAIPP
                     IAMTILYSPQKTIWVIAFLFILQQLDGNFIEPKVMGNQVGIGAIWVISAILVGQSLFG
                     FIGVFLSVPIAAVVKTYVDKYIDNRLQ"
     gene            8606..9322
                     /locus_tag="L3M60_002416"
     CDS             8606..9322
                     /locus_tag="L3M60_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HBN6227071.1"
                     /translation="MRINKYIASCGIASRRKAEEIILEGRIKVNGSIVKELFFNVDEE
                     KDIVEFDNKKVKPSENYIYIVLNKPEGYITTVKDQFNRPSVIDILKDVKERVYPIGRL
                     DYETSGLLILTNDGDLTYKLTHPKHEIDKTYVASVKGIISGDEIRKFETGLKIEDYTT
                     APAKIKVTKENKEKNYSVCEITIHEGRNRQVRKMCKAINHPVLNLRRISVGKIVLKDT
                     KVGEYRYLTEDEIKYLKSIK"
     gene            9379..9957
                     /locus_tag="L3M60_002417"
     CDS             9379..9957
                     /locus_tag="L3M60_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBN6227072.1"
                     /translation="MAYAKYLTKITDINKFYLENIINEGDIVIDATMGNGYDTRYLAE
                     KVGEKGFVYAFDIQEEAIKSTRKKLEKEGYTDRVKLIFDGHEKMNQYIKEEVSCVLFN
                     LGYLPRAKHSIITKPDTTLKAIKLSLEMLKENGVISIAIYTGHDGGQEEKDCIYNLVN
                     NLNQDEFNVLESKFLNQVNNPPQLILIEKKKA"
     gene            10351..11223
                     /locus_tag="L3M60_002418"
     CDS             10351..11223
                     /locus_tag="L3M60_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBN6227073.1"
                     /translation="MEETNEMKDSKHLISNIQSQYTRLSKGQKLIAQYILNNYDKVAF
                     MTACKLGETVGVSESTVVRFANALGYSGYPKLQAALQELIKNKLTTVQRVEMAHDYSD
                     DFAILNKVLKSDIDNIRSTLEEIDERAFKEASNKLLRARKIYILGMRSSFVVAQYLGF
                     YLDIILDNVHIIRMDMGDAFEQIVRINEEDVIVAISFPRYSKKSYQIVNYAKEKGAHV
                     ISLTDSLFAPVASLADNTLLVKSNMASFVDSLVPALSISNALAISVGMKEKEDIKQHF
                     DDLEQIWKRYSVYE"
     gene            complement(11376..11561)
                     /locus_tag="L3M60_002419"
     CDS             complement(11376..11561)
                     /locus_tag="L3M60_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBN6227074.1"
                     /translation="MKIHNLRYTGASLLHLVGTNMKIVSERFCHTDLKLTMNIYSYVL
                     EEMYKKTSNNLSKILFR"
     repeat_region   11859..12825
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=11859..11887
                     /rpt_unit_seq="gttttatattaactaagtggtatgtaaat"
     gene            13463..13747
                     /locus_tag="L3M60_002420"
     CDS             13463..13747
                     /locus_tag="L3M60_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227075.1"
                     /translation="MIVYNKNFYPNDIFSRLDFSKIKRQLKLIDNELSDFGNICIIEK
                     EHYTISVNSIGEINVYYDLEYENKVYGIVEEIEKLFKSQVGKFSISTYRN"
BASE COUNT         5918 a         1456 c         2336 g         4765 t
ORIGIN      
        1 aaactacatt tattatgtta gaatatctta tgatattaaa ttgtaaatgt ataatattat
       61 ttagttacta gattgggggg aaatgatgaa taaagtctat attgtaacaa catatacagg
      121 aactatatta tcttacttaa taaggaatat aagtaaaaaa ctgtatacac atgtgtcaat
      181 atcattaaat gaaaatctaa aaccaatgta ttcttttggg agactaaatc caaggaatcc
      241 ctttattgga ggttttgttg aagaaaatat aaatcaagga ttatatgcta taagaaaaaa
      301 cactgtgtgt agggtatatt cgttagaagt agataattta caatatgaaa atctatataa
      361 aaatataaaa tcaattagtg attatagaga agattactat tacgatacaa tggcattaat
      421 atatttacca atgaatattc acagacactc tgaatataaa tatgtatgtt caaattttgt
      481 tgctgacatg ttagaaaaaa gtgagataga cattttaaat aaacaagcat ttgaagtaac
      541 accaaatgat ttttataatt tatctggttt aaccttagag tatgaaggtt tattatcaga
      601 atataattct agacaacatt cttatgtatc aaaaatcgca aacatttaaa aacttgattt
      661 agagtttttc atataaaaaa tattgctaga caattaggct gaagatagta gtttaacttc
      721 gataggatta agaatttgta aatcttagtt agaatacttt cttcagtttt taaatttctg
      781 ctattatttg tatttttata atatttttgg atatatatat tacatataat tgaataggta
      841 gaactataaa tatataaaga tatcttaata cataataaat atgataaata tttatttttt
      901 gtaaatatat ggatataata gtaataataa cattttttag agaggagtat taaatgaaac
      961 gatatcttaa aacatatgga gttgcattaa taattgtaat aatttatgca tttattaaat
     1021 tacctgtact aagattagat tttacatctt tattttcagt aggaataatt ttctttgttg
     1081 tagcaggaat actagatatg atgttagata gaaatgaaag agcatctaaa atggctaaat
     1141 ataatttttc aatagcaata gctttgcttg tgtatattgt ggtagttccc tttataactt
     1201 ctactcctgt atttcatgca aaatcatata gagaactatt gggaaaagta actgaaagta
     1261 aatttactga tgacattagc cctgtcagtg taaatgacat tcgtcttgta gatgaagata
     1321 tggcaatgaa attgggcgat aaaaagcttg gagaagttcc tgcaataggg agtgtatcca
     1381 aattaggaaa atttcatata caaaatgtag atggagagct gtactgggta gcacctcttg
     1441 ttcacaggga cataattaaa tggataacat ctctaagtgg tacaagtggt tatgtaaaag
     1501 tatcagctag taatcctcaa gatgtccaat tagttcaaga aatagatgga aagccaataa
     1561 agatagttta ccaaccagaa gcctatttac atcaagattt acaaagacat ttatacatac
     1621 atggtattgt aaatgttggt atgacagatt tcactcttga aataaatgat gaaggaagac
     1681 cttattgggt tgtaagttta tatgaacata aaattggata tggaggagca aatgccacag
     1741 gaatagccac tgttgatgca gaaactggaa aaataaatgt ttatgatgtt aaaaacacac
     1801 ctaaatgggt ggatagaata caaccacaaa actttataac agaccagatt aaagactggg
     1861 gcgtttatgt taatggattt ttaaattctg ttatatcaga aaaaggagtt ttagtaccaa
     1921 cagaaggtac atcattagtt tatggtaatg ataataggtc ttattggtat acaggtataa
     1981 catcctcagg tggagatgaa tctacaattg gatttatgtt agttgattct agaacaaaag
     2041 aagctagatt atataaacaa ccaggagcta ctgaaacggc tgcaatgaaa tcagctgaag
     2101 gtaatgttca agaaaaaaat tatgaagcaa cattccctgt aatgtataat atattgggtc
     2161 agccaacata tgtatcatca ttaaaagata aggctggtct tgttaagaga gttgcatttg
     2221 tatctgttga agattataat gtacttggaa taggtgaaga taagaatgaa gcattaagaa
     2281 actataaaga tgcattagaa tctaaaggga atgatttgaa acttgataat gaattaaaag
     2341 atgaggtaat agaaggtaca attacaagaa taagcccaga tgtacgaggt ggtaacacta
     2401 attattatgt aactttagat acaaataaag gtataatatt tagggctact tcaaaagtat
     2461 ctagcgaatt gccattgact caggttggtg ataaagtcaa aatatcgtat tcaaaagaag
     2521 aatctggtgt aatagaaatg tcagaatttg ataatctaaa tattgcaaac tttgaagata
     2581 aaaaagcaaa agaaggaaca ggaactgatg agaatacgga ttctcaagat tctgaaaatc
     2641 aaggtaatgt ataatttaaa taaaaagact tataacaata tttaacatag taatagctaa
     2701 tttttataga atttaattct ataaaaatta gctattttat tttaattctt tgtaaattta
     2761 atgtcataaa attaatataa tattagtaca actttgcaaa taatacttca aggagaatgt
     2821 actatgacga atatggaaaa agagatatta cttttaatta gcagggttag atgtgttaca
     2881 gagtctcaat ttagaaaaat ttatggagac caaaaaagat acaataaaaa aaattttaaa
     2941 aaaactttga gaaaaatgtg tagtgaatat acactaaaaa aatatccttg tgatgtagat
     3001 tattataatt ataaagacct ctcttatgta tactatttaa atggtagtaa gctttttaag
     3061 ggagatgatt taataaaggc tctagtagga tcagaattag ttgttagaat caatactgca
     3121 ggttgtgatg taaaaagatt ttaccgaaat gttagtgtag atgatgttaa atacgattta
     3181 tttattgaat atacagacca atttggtaat ataaagcaag ttctagtaga tgttgactta
     3241 aataatgaaa tagacatttc taaatacgaa aatctttcta aaaaaataga taaatctaca
     3301 attccatttt ttaaagtgcc aaacgttttg gtagttacac cagataactt atctgaatta
     3361 aatgatttag agagatatga taatgagtat agtataaatt ttatagattt gggattaaat
     3421 aaactattta attgtatata agctaatttt agaaaagaaa actattgatt tgccacattt
     3481 tgtaaaataa ttgttgcaat attatataga atatgataaa actatattaa taaattaatt
     3541 aaaaataatg agcctagggt agaggtagct gtattcaata gtattcaata ggagctggta
     3601 agcattgata attgatgaaa gggtatatag ccgaagaaat tttgttgacg gacaaatttt
     3661 ctggacttat gcagaatatg cataaggctg tcacttttaa agtggtgagc tacaaggtta
     3721 cttggtctat gaaatttaca cgtccaaagt atacctttgg gcgtttttat ttatcaaaat
     3781 ttagcttgag ggggagaaag catgagtata attgtccaaa aatatggcgg aagttcagta
     3841 gcagatactg aaaaaataaa atcaatagca gaaaacataa tagaacgaag aaaagaaaat
     3901 ccacaaatgg taatagttgt gtctgccatg ggaaaatcca ctgatgaata tataactttg
     3961 gcaaaggaat tgagtaatga acctagtaag agagaattag atgctttaat gtctactggt
     4021 gaaatgattt cagcatcttt attatcaata gcattaaatg cacttggatg taaagctata
     4081 agttataatg cttatcagtt aaatataaaa acgagtggtc ttcatggaaa gtctcagatt
     4141 gatgatataa acgtaaacag aataaaaaat agtttagatg aaggaaatgt cgtgatagta
     4201 actggatttc aaggaatcaa cgaagatggt gatgtaacta ctcttggaag aggtggttca
     4261 gatacatctg ctgtagctct tgcagttaaa ttaaatggaa aatgtgagat atatacagat
     4321 gttgatggaa tttattttac agacccaaga aaatattcaa aggctagcaa gcttgatgag
     4381 attgaatatg aagaaatgct tgagttagca agtttaggag cacaagtaat gcattcaaga
     4441 agtatagagc ttgcacaaaa atatggtgtt gaaatttatg ttggacgtac atgtggtaca
     4501 gaaaaaggaa catatataag agggggaaaa gacatgaaat tagaggataa agtaataaca
     4561 ggattagcta ctagtgatga tgacagttct ataacaataa aagattttaa agcagagaat
     4621 atatctagct tatttgaaga tatagcaact ataggtataa gtgtagatat gataagccaa
     4681 actgcaccta ttttagataa gataagtgta tcatttacag ttccaaaaga agaattggga
     4741 gaatgtaaaa agatagtatc taaatataca gatgaagaac atgtagttat tgataataat
     4801 atcactaaat tttccttagt aggtcttggt atgaaaaata cttctggagt tgctgctaaa
     4861 gtatttaaga tattcaatga aaatggtata atgataaaac ttataactac atcagaaatt
     4921 cgtataactt gtgctataaa ttctgatgat aagcaagtag ctatagagaa aatagcagaa
     4981 gtatttaata tataaaattt ggggaggcta ttatgaaact tcatggaact atgactgtaa
     5041 ataatgatga attatatatt ggaggagtga gttgtttaga acttagtaaa aaatatcaaa
     5101 ctccacttta tgtatttgat gaagctttag ttagacaaaa ttgtagagaa tatataaaat
     5161 attttagagc tagagaggga aaaaataagg ttgcttatgc ggggaaagct tttttacctc
     5221 tttatatgtg taatcttata aatgaagaac aaatgtgtct agatgtagtt tcaggaggcg
     5281 agttgtatac agcttataaa tcaaattttc ctatggaaaa agttttcttt catggaaaca
     5341 ataagactaa agaagaaata aacatgggat tagatttagg tgtaggtaaa tttgtagtgg
     5401 ataattttta tgagttagat ttacttgaag cattatgtga agataaaggt aaaactcaag
     5461 agatatattt tagaataact cctggtatag aagctcatac acatgattat ataaaaactg
     5521 gacagataga ttctaaattt ggatttgctt tagtaaatgg agatttattt agagcaatag
     5581 aaaaattaag tgactataaa aatatcaaac ttgtgggtct tcacgctcat ataggttcac
     5641 aaatatttga tgtagaacca tacatagata cagtagatat aatgatgaac ttagtaaaag
     5701 aaataaaaga aaaattcaac atacatctaa cagagattga cctaggtgga ggcgttggag
     5761 tttattatag cgaaggagat aaacctaaaa ctataaaaga gttttgtgaa actataatca
     5821 ctaaagcaga aacttcatgt aaagagcttg gaatagaagt acctacattg gtaatagaac
     5881 caggaaggtc aatagttgca aatgcaggaa gtacattgta tacagtaggt tctataaaag
     5941 atataaaaga tgttagggta tatgtaagtg tagatggcgg tatgacagat aatgtaagac
     6001 cttcgttgta ccaagctagt tatgagtgta gtattgtgaa taaaattaat cacaaggcta
     6061 tgaatcatgt aacaatagct ggaaaatgtt gtgagagtgg agatatatta attggagata
     6121 taaatataat ggacattaaa agtggagata tacttataac aacatcaact ggtgcatatg
     6181 gatattcaat gtcttcaaat tataataaaa taccaagggc agcagtagta tcagtatttg
     6241 acggaaatga taagataatt tgtaagagac aaagctatga ggacttatta gctttagaag
     6301 tataaaatgg agaagcatat agtaaaaatt gaatattaaa gtttattata agcagatttg
     6361 ctatgatata tatctaaatt aatttttggt ttattatatt gttcgtagca tatctgctta
     6421 tttttcgttt tactaacaaa tttcttgtaa taaatataaa atattttaaa aactttataa
     6481 ttatgataaa atttaaagta tatgacaagg agggatttaa tgagaggaag agtaatttta
     6541 ttaatatcga tattatctat atttcttgta ggatgttcat ttaataaaaa agatgaaata
     6601 aatttagtag agcaaggaaa aacgtattta gaaaaacata actataaaaa agctatggaa
     6661 tcattatcct ctgcactgga agaagatagt actaatgaga atgcaagagc tatgtatatg
     6721 caagctatga gaatgtcaaa tatgacagag tttgaagaat taaaaaatta tgagggggca
     6781 atcaaggaac ttaaacttat agagaaaatc aaaaatggtt cctctgttat taaagaagag
     6841 gcaactaaaa agaaagaaga gttaactaaa ttagaaaaag aacaaaatga agcagaggaa
     6901 caaagaaaga aaaaggcaaa agatacagct ggagaagata ggtatagagt tgaatcagat
     6961 gcaagaaaat cttcttatag tggaagtagt aaaaataata aatctagttc taaaaataat
     7021 aaaaaatcag atagtgataa atctgacaat aataataact cttctcaaaa tcaagaaaag
     7081 ccaaatagta gtaatccaac accagcacca accccacaag atactagtgg tggttcagat
     7141 agtagtggaa attctcaaaa ctcgaataat caataagtat aaaatatata cataaatttg
     7201 atgaacaaaa tatatattat aaaaaatacc tcttagtaag tttttcttac taagaggtat
     7261 ttttttaaaa ataatttgac aaataacagt taaaatttaa actaaaagct aaataacagt
     7321 agagataaac tattgcaacc gattgtctat atatttatca acgtaagttt ttacaacagc
     7381 agctattggc actgaaagaa aaactcctat gaaaccaaat agagattgac ctactaaaat
     7441 agcagatatt acccagatag ctcctatacc aacttgatta cccataactt ttggttctat
     7501 aaaatttcca tctaattgtt gcaatataaa tagaaatgca atgacccata tagttttttg
     7561 tggactatat aatatagtca tggctattgg agggattgca ccaataaaag gaccaaaata
     7621 tgggattata ttacctaaga atattattga agctaaaaat aaagcatttt ctattctgat
     7681 aacaaattga aatcctaaaa atgcaattat accaataata agagaatcta atattctacc
     7741 aagtaaataa tcatagaaaa tatcatgagt agtcctaaaa aattctataa tgctatctgc
     7801 tttgtttcta gtagtagaag aatatagaaa tctcttaaat cccaatgcaa ttttttcttt
     7861 gtcatatata atgtatatag atagtataaa tcccattgtc atattaaata gcatagaaga
     7921 tatgctaagt gtataaatta agaagttttt agaaatttta ccaataaaat tacttgcttc
     7981 ttttaataca ttatctaaac tatgttggat atgaggaatt aaagttttta gaaaatctat
     8041 gtttgacata tttttttcta tgaaactatt tatataatca gtatacatag gcatttcatt
     8101 tatcaatcta tttaatgtat tagctattga tggaatcaat aacctaatgg caaaggctaa
     8161 aataaaacct ataaatatat aagataaaaa aatagctaaa agtctatgtg ctttgaattt
     8221 tacctcaaaa agcatcacaa gtggatttaa aagtaaagca agtaaaatag ctaataaaaa
     8281 tgggctaaaa aactttaaga aaccttcaat actagatata aagtctgatg gagtattaat
     8341 aaatttaaaa aataaaattc caataaacac gactattaaa taatatttta tattttcttt
     8401 tgaaataata atcacttcct taaatagtat ctagtcagat attgcatatt aatagtatgt
     8461 gaaaagaaat tacaattatt taggaaaatt aaataatttt ttggcttgtt tttaattaag
     8521 gtctagtata gtataataat gtagcaaaat taatattgat aaaaatatta aacagcacat
     8581 aatttaagat aaggaaggat taagtatgag aattaataaa tatatagctt cttgtggaat
     8641 agcttccaga agaaaagcag aagaaataat tcttgaagga agaataaagg taaatggaag
     8701 tatagtaaaa gagctatttt ttaatgttga tgaagaaaaa gacatagtag aatttgataa
     8761 taaaaaagta aagccaagtg aaaattatat atatatagta ttgaataaac cagagggata
     8821 tataactaca gtaaaagacc aatttaatag accaagtgta atagacatac ttaaagacgt
     8881 aaaggaaaga gtatatccaa tagggcgatt agattatgaa actagtgggt tattaatact
     8941 tactaatgat ggagatttaa catataagct tacccatcca aagcatgaaa ttgataaaac
     9001 gtatgtagcc agtgtgaagg gaattataag tggtgatgaa ataagaaaat tcgaaacagg
     9061 gctaaaaata gaagactaca caacagcacc tgccaaaata aaggtaacta aagaaaataa
     9121 agaaaaaaat tattcagtgt gtgaaataac tatacatgag ggaagaaata gacaagtaag
     9181 aaaaatgtgt aaagctataa atcatccagt tttaaactta agaaggattt ctgtggggaa
     9241 aatagtatta aaagacacca aagttggaga atatagatac ctaacagaag atgaaataaa
     9301 atatttaaaa tcaataaaat aagataaaaa taaattgaga aaaaagaaat aaagttaata
     9361 gtagggagaa atagtattat ggcatatgca aagtatttaa ctaaaattac agatataaat
     9421 aaattttatt tagaaaatat tataaatgaa ggtgatatag taattgatgc tactatggga
     9481 aatgggtatg atacaaggta tctagctgag aaggttggag aaaaaggctt tgtatatgct
     9541 tttgatatac aagaagaagc aataaagtct acaagaaaaa aattagagaa agaaggatat
     9601 acagatagag taaaattgat atttgatggg catgaaaaaa tgaatcaata tataaaagaa
     9661 gaagtttcat gtgttttatt taatttaggt tatcttccaa gagcgaagca ttcaattata
     9721 acaaaaccag atacaacttt aaaggctata aagttgagtt tagaaatgtt aaaagaaaat
     9781 ggagtaataa gcattgccat atatacagga catgatggag gtcaagaaga aaaagattgt
     9841 atatataacc ttgttaataa tttaaatcaa gatgaattta atgttttaga atctaagttt
     9901 ttaaatcaag tcaataaccc accacaattg attttaattg aaaagaaaaa agcataataa
     9961 atactaaaaa acttgacaag attctattaa gagttaatat atatagtgtg acctattaaa
    10021 aatactttaa ataataagta tacaataact tatactcaaa acaaaatata catctaaata
    10081 tgcaaaataa gatagataac ttttaaatga gtaaaaattg aaaaatttaa aagaaatatt
    10141 ggataaatat taaaaaatgc aaggaaagat ggtataataa atttaatcga aaatactatg
    10201 tgaagtataa ggtacagaaa agttagaatt ttatgagttt aagaagctat gataaaaact
    10261 aagatgaaat tgagataaaa aataaaattt ttagagaaaa agacagtgtt aataaaacaa
    10321 aatagtttat ataactaaaa ggagaaaaaa atggaagaaa caaatgaaat gaaggacagt
    10381 aaacatttga tatcaaatat acaatctcaa tatacgagat tgagtaaggg tcaaaaactt
    10441 atagcacaat acattttaaa taattatgat aaagttgcat ttatgactgc atgtaaattg
    10501 ggtgaaactg taggagtaag cgagtctaca gttgtaagat ttgcaaatgc attaggttat
    10561 agtggatatc ctaaattaca agctgcactt caagaactca taaaaaataa actaactaca
    10621 gtacaaagag ttgaaatggc acatgattat tctgatgatt ttgcaatact aaacaaagtt
    10681 ttaaagagtg acatagataa tatcaggtct acattagaag aaattgatga aagagcattt
    10741 aaagaagctt ctaataaact tttaagagct agaaaaatat atatacttgg aatgagaagt
    10801 tcttttgtag tagctcaata tttaggtttt tacttagata taatacttga caatgttcat
    10861 ataattagaa tggatatggg agatgctttt gagcagattg taagaataaa tgaagaagat
    10921 gtaatagttg ctattagttt ccctcgttat tctaaaaaat cttatcaaat tgtcaattat
    10981 gctaaggaaa aaggagctca tgtaatatca ttaacagata gtttatttgc accagtagca
    11041 tcacttgcag acaatactct tttagttaaa agtaatatgg cttcgtttgt agattctcta
    11101 gttcctgctc ttagtatttc aaatgctctt gctatatctg ttggaatgaa agaaaaagaa
    11161 gatattaaac aacattttga tgatttagag cagatttgga aaagatattc tgtgtatgag
    11221 taaagtttgt atattaataa atagtttcaa gagttaaaat gtgttcaaat ctagccctat
    11281 ttaagcaaag atattataaa ataactgact ggcatttaac tggcaaattc cttttttagg
    11341 tttaactgac atttaactga cagtcagttt tttgtttatc tgaataatat tttacttaaa
    11401 ttattagagg tctttttgta catttcttct aaaacataag aatatatatt catagttagt
    11461 tttaaatctg tatgacaaaa tctttcagaa actattttca tattagttcc taccaaatgt
    11521 aacaaactag cacctgtata tcttaaatta tgtattttca tttgtcatta ggtattttta
    11581 atacattata taactatgat tttattattt gaaatctaag ttataaatac tattgatgat
    11641 agagtaattg tatacaaaaa tatataatat atttatgata taataaagat atagaaattt
    11701 tgcagtgttc gattttttga agaaaatatg tttaagcatt gaaatgtaag gtattgatag
    11761 tgattgataa acattatcat ttgcactact gttcgctcac tgcaaattta agagaattgc
    11821 atatgtgtga gtattggaaa ttctacgttt attttagggt tttatattaa ctaagtggta
    11881 tgtaaattta agcttaaaga ttatggtttt caagttttag ttaagtttta tattaactaa
    11941 gtggtatgta aatttaagaa atgcagataa taggatacct ttcaactggg ttttatatta
    12001 actaagtggt atgtaaattg gaaactaata tttatataaa acttgtaaat atacaggttt
    12061 tatattaact aagtggtatg taaatcgaga aattgagcta attttactat tatcagcatt
    12121 gttttatatt aactaagtgg tatgtaaata aaggtgtgaa aagagatgag ttaaatcaaa
    12181 gtgaagtttt atattaacta agtggtatgt aaatgaaacg aagctagtta ttgtgattat
    12241 acaagtttcc gttttatatt aactaagtgg tatgtaaatt taatagcaca tcttcgcttt
    12301 caagtttctc tatcagtttt atattaacta agtggtatgt aaattatata ttggatgtca
    12361 atgtcaagaa tacaaaattt agttttatat taactaagtg gtatgtaaat ataggaaaat
    12421 agagataggt tcacaatcta atgataggtt ttatattaac taagtggtat gtaaatattt
    12481 tttcttttag ttttttagga actggtaatc cacagtttta tattaactaa gtggtatgta
    12541 aatttctgaa tcatatgaaa acaaaatctt atttgtattg ttttatatta actaagtggt
    12601 atgtaagtga aattattaat gttatttcta ctcttgcagt tgcgttttat attaactaag
    12661 tggtatataa agcatagtct attgaacatt tattaagaga taactttcgt tttatatagt
    12721 tttatattag ctatattgaa tttaaatgtt tcactattgg acaacggaag ttttttcagt
    12781 gagtaaagat tgaatcactt tatatttatc ttaattatgt gataatataa ttattatctt
    12841 atttctattt gtaacttttt taagtatgta ttgtccagtt gtaatttgtt tttattattt
    12901 taaactttca attccaaaat tatctaatat aattgaatta acttactttc tttttttatt
    12961 ttacctcctt gttctctaat taatttatac attttattta tatttaagaa tacatactct
    13021 aatttgatta cataaaataa aatgtttttc tactataaat taaactcttt aaaaaatatg
    13081 actcaactac tttaattttt attctaatga tacattaatt ctagacataa taaaactctc
    13141 ctagctagat gattttacat gttttaacta tctcctaaaa aagtatagtt tgataaaaag
    13201 agcattttta tataagcacc atacttttat tgtggaagat aattatttta tgaggtattg
    13261 aaaacttaca taaaatctgt ggataaaaac tcatactttc attttactga catacatcta
    13321 aacataataa tataaaacat tgaaaaatcg atttgcaatc tgtttttata taagaaaatt
    13381 tgaataatat ggaaaaatat gattaatgca ttattaatta gggtataaag tatcataaat
    13441 agaatgataa ggggtggatg aaatgattgt atacaataaa aatttctatc ctaatgatat
    13501 attttcaaga ttagattttt caaaaataaa aagacagtta aaattaatag ataatgaatt
    13561 gtcagacttt ggaaacatat gtataataga gaaagagcat tatacaataa gtgtaaatag
    13621 tataggtgag ataaatgtgt attatgattt agagtatgaa aacaaggtgt atggaatagt
    13681 tgaagagatt gaaaagttat ttaaatctca agttggaaag tttagcatat ctacatacag
    13741 aaattaatac ttgaaagtta gaaattaaaa gatttaatac taaagtatag ataaattatt
    13801 aggtaacaac taaaaagagt tgttaccttt ttcaaataag tatatattat ttttattaat
    13861 tttgatatac cctaatttat atttttatat ttgttttata tttttttgta aagagaagat
    13921 gaagaaaaaa caatgatata aatataagta tttctaaaat aatataataa ctaagcgtta
    13981 ttttagaaaa gttacaaata atatctaaac tattaaccaa attatgaatt atagaacaaa
    14041 acagaatatt tttggctttt atataaacat aacctaaaaa tatataaagt ccgattccat
    14101 atatcaaatg aattaaactt taaattgttt gagtgtctag attatacaat gtgaattgta
    14161 agggtaaata cataaacttc aacaaatatc taccactata atacctattt ttttaacgaa
    14221 tttatcaaat aaaacatttt gtaagaaaca tctctaacca tattcctcag ataaaaaaga
    14281 ataaatataa actaatgata cttatagtaa gatttattag cttaaatgat tgaaaaataa
    14341 aaagtaaaca acaatcaata aaaaacaata ttactaatcg ataaaattta taattttcat
    14401 tagtttttct caacatataa aactttgcaa tagcaactga agatagtgat aaaaccatca
    14461 taaattttga aaaac
//
LOCUS       DAFNNB010000097        14126 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0098, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000097 DAFNNB010000000
VERSION     DAFNNB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 14126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0098"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(61..582)
                     /locus_tag="L3M60_002421"
     CDS             complement(61..582)
                     /locus_tag="L3M60_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBN6227076.1"
                     /translation="MVLLIVDTQKLITNEELYNFNEFVVNLVKLIDTARKKYIEVIYV
                     RHDDGIDCELTKGKHGFEIYEEFKPCDEEKIFDKDVNSAFKETGLLEYLTNKNEKDII
                     IAGLQTDYCIDATIKCGFEHGFNIIVPSYANTTIDNSFMSAEQSYKYYNEFIWNGRYA
                     ECISLDETLKRMN"
     gene            complement(1208..2062)
                     /locus_tag="L3M60_002422"
     CDS             complement(1208..2062)
                     /locus_tag="L3M60_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HBN6227077.1"
                     /translation="MVMGFEFKIMRSLIYVGLAKEEYRPKLMDWLYRHHIPDSISTFG
                     PYCTKYAFYQAYPTPNEGERFGARKMQLTEHYWLVDEHMPEMANRIMTEYMPMDVLRW
                     QGCIPDIENKRVHENAESGDAGRAVGGDNGCPPFIFAFVPINWEEDFRGKGRTVQDGP
                     NYRWQFMIKYPDGVSKEEGEKWFYDEVVPYFTNCCYVNRFVSSKIMINYGATAFDRVA
                     ELWFEGEEEWYKAVVEEAKSFIKKPEWAQEEEFPYLKPQFNIASVFLGDIATMDAYSQ
                     YRGYIPMR"
     gene            complement(2094..2606)
                     /locus_tag="L3M60_002423"
     CDS             complement(2094..2606)
                     /locus_tag="L3M60_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBN6227078.1"
                     /translation="MDRKKKEILTLSMGIALLPPLWAVLAPYIGIKTGAVALICAGLY
                     VTNGNKQKDGLKIMFGFWCGDLWAVLAILIMEYMNFNQNLELFLTLSILGFFAVVIAS
                     SFEKIIFLPSWLCGWAIGLTIMTGENIINLQDICIQIAIAMAVGIWYVGVGVDLFTRY
                     ILSKDKKKLN"
     gene            complement(2599..2823)
                     /locus_tag="L3M60_002424"
     CDS             complement(2599..2823)
                     /locus_tag="L3M60_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227079.1"
                     /translation="MNEKPSFKQIEIILVVICLVFAHYNVRLTPEFIAIIIPLYLSYF
                     IVSIIANQIAIKLLEIRQTREATSYNIGNG"
     gene            complement(2876..3829)
                     /locus_tag="L3M60_002425"
     CDS             complement(2876..3829)
                     /locus_tag="L3M60_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBN6227080.1"
                     /translation="MKILGISFGTKNGNNDTMCKVALKGAKEAGAEVEFIQMSSINIK
                     HCTGCCACVKTLLSGKGSICVLKDDFEWLLDKMKDADGIVVSDPIFEEGASGLFHTIM
                     DRFGPRADRGNNVIGTKVAEATGGKIPDSRMLNDKVISFMGIGGSDWGTRVQCEHAML
                     ALIPMWKVIDNAWFPWAKELVMDDTKLAQVRQLGLNIVEAAKDFEKASYQGEEGVCPH
                     CHNRLFYIEPGTKKAICALCGIVGELDTISGKTIFSFPEEQLGHAHDTLPGKFIHGDD
                     IKKMEGHYAEVRKTPEFKERKTAYNFIEPLTKEKQAGASLR"
     gene            complement(3857..5245)
                     /locus_tag="L3M60_002426"
     CDS             complement(3857..5245)
                     /locus_tag="L3M60_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227081.1"
                     /translation="MEDKFYAKGKGNNGYIKNLEVCSFNNLDGTCGMFQMALYKRDEK
                     YYLYGCCFGGNKKNGVMISDITDPYNPQFIKHFQMLDPKEYPTTTTPKIQIADDLMIV
                     AMSCGSGPGALVDQAKLANIKCEAGIRIYSLKEDPLNPKFLGYWDCGLKHVMGVHRFM
                     YNGGRYVHLSSDCVGFEGLVYRVIDIINPTNPVEIGKWWRPDQYADGYPNRTFDAGAP
                     HCPEFMDKGWLHGPPFVRDGKAYCGYGGAGLIILDVEDLTRPKCLGELPFMPAFSSRL
                     AGARTHTALPLPGRDLVVVQNEGERFQFFKPDNITDVQAMNNIHMVDVSDPTKPTLIA
                     QFPYPEVPKDFPYPNFNVAGLGKPGPFGPHNLHEPMDNKSWLEQRGDRVYCCYFHAGL
                     RVYDVSDPYYIKELAYFIPPNPNKTPEESYFPGFLGPRLAVTEDIIVDDRGYIIIDAL
                     DDGFYILKMKED"
     gene            5518..6399
                     /locus_tag="L3M60_002427"
     CDS             5518..6399
                     /locus_tag="L3M60_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6227082.1"
                     /translation="MNTKQLEYFISVAENLSFTKTAEKFYISQTAVTQQIKSLEEQIN
                     VTLFTRSKRHVELTPAGKVFLSEARTIIKNINDAIAKTQQFAHGFFGTLSIGTLIGYE
                     KNKLQQYLKEFSNTYPNISMDICTNEITELLNLVKNNSMDLAFVINPENQPLKDFEHK
                     TVERYSLVALLPPGHPLYNEDSIDLIELQHDKFIFVKETGDEYGQKSMIQNRYREAGF
                     VPNVVQRSNNLNTIESLVASNMGISILPSFCVTDTMIERDIAIVPIKEVENRIEVVVV
                     WNKKNTNPALEKFISII"
     gene            complement(6587..7579)
                     /locus_tag="L3M60_002428"
     CDS             complement(6587..7579)
                     /locus_tag="L3M60_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBN6227083.1"
                     /translation="MNILAFGEIMMRLSVPGYKFLTQTNELNYIITGTGLNILSGLKN
                     FGYNTYMLTKLPNNNVGKASSANVRKLGVKDDFITYGGNHIGVYFLESGYGERPSEVT
                     YLNRLNSSFCESKLQDYDIDKCLEGMDVVHICGIAMQLTDSVKEIALELARKSYEKGI
                     KVFFDFNFRQSLNPERSYDDLVNDYKKILPYCHGLFASCRDINKILNIDNLDIKSSSE
                     LIKKEFDIDIVAGTMRRFDDMNNRFIKGYISNDDGYFESKEYKLEIYDRVGAGDGYVA
                     GIIYSYLENMSNEDIVEFGVASSVLAHTTYGDNSLVSTSHVLRLMNNEKIDILR"
     gene            complement(7584..8324)
                     /locus_tag="L3M60_002429"
     CDS             complement(7584..8324)
                     /locus_tag="L3M60_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HBN6227084.1"
                     /translation="MNCTPQYYKNRVCLNVLAGSIKNAKEVYDACDGHILVGLLSKNY
                     STVEVAIEDIKKYSKEIDNAISVGLGAGDPNQSNMVSQISKELQPQHINQVFTGVATS
                     RALLGQDDSIINGLVSPTGKPGFVKISTGPLSSKEKDGIVHIETAIAMMKDMGGSSIK
                     FFPMGGLKTKDEYIEVAKACAKHNFYLEPTGGIDLDNFKEIVQIALDAGVEKVIPHVY
                     TSIIDKETGDTKVEDIEVLYKIMKELLG"
     gene            complement(8389..9498)
                     /locus_tag="L3M60_002430"
     CDS             complement(8389..9498)
                     /locus_tag="L3M60_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HBN6227085.1"
                     /translation="MSSIFEKYSVRRVINASGKMTILGVSTVDKEVIEAIGEGCKNFF
                     IIEELVNKTGEYIANLLGCESALIVSSASAGIAQSVGAMIAKDDMSLVYNVNNPKKQV
                     KREIIIPKGHNVDYGVPVEVMIRLGGGEVVEAGYANMCNKEHIIREINENTAAIMYIK
                     SHHCVQKSMLSVLDAVQVAKEYNLPLIVDAAAEEDLNVYYELGADLVIYSGAKAIEGP
                     SSGLVIGKKKYIDNVKLQSKGIGRAMKIGKENIVGLTCAIERYISNDKVTLKEMEDKL
                     NPFIDKINTLKGVSASITRDSAGREILRGEIDFNEDIINKSTQQIINELRGGEIAIYT
                     RDYKANEGKIEIDIRSVTGGDLDIIYNKIKEIIEK"
     gene            complement(9516..10649)
                     /locus_tag="L3M60_002431"
     CDS             complement(9516..10649)
                     /locus_tag="L3M60_002431"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HBN6227086.1"
                     /translation="MKIDILIKGGKTVDKSIIDIAILDDKIIEVKNQIDDSKYQANQI
                     INLDGEKFISAGWIDIHTHCYERLDLYKDYPDEVGIKSGVTTVVDAGTTGALDIGKFY
                     EDTKKYKTNVYALINIAKQGITSQDELSNMMNIDEDELKNAVKKYKDFVVGIKARMSK
                     SVVISNDIKPLKVAKRIKNELNLPMMVHFGSSPPMIEDIFDYMEKGDILTHIYNGKPN
                     GILRGNEVKKEIIEARERGIILDVGHGTESFSMDVAMKSKDAEIFPDTISTDIYIKNR
                     INGPVYNLSTTMEKFIYMGYSLEDIINKVTKNAAKAISLKNKGLIKEGYDADLTIFDV
                     VDEEKELQDSLNKSVITSISIKPRAVVVNGEYLNIGKSIRENE"
     gene            complement(10651..11745)
                     /locus_tag="L3M60_002432"
     CDS             complement(10651..11745)
                     /locus_tag="L3M60_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HBN6227087.1"
                     /translation="MERRLGISVYPEHSKMEDDKAYIKMASKYGFKRIFMCMLSITRP
                     KEEVKKHFKEIITYARELGFEVILDIAPNIFDILEISYDDLTFFSELNASGIRLDYGY
                     DGSKEAMISFNPYDLKVELNMSNDVAYLDNIITYKPNVNNIYGCHNFYPQRGTGLPYD
                     FFVRCSNRFKKYGIRTAAFVNSKYATIGPWNINDGLCTLEMHRNLDIATSTKHLFATG
                     LIDDVIVGNAYASEDELKRMSEVDRYQVVFNVVAKEKTSEIEKTIMVNEQHFRRGDIT
                     NQVARSTEVRKKYAKENFPKHDNTNIFKRGDVVIGNDDFGKYKGELQLILQDCTDERK
                     NKVGRIKEDEIILLDFIEPWSKFKIILEGE"
     gene            complement(11883..13184)
                     /locus_tag="L3M60_002433"
     CDS             complement(11883..13184)
                     /locus_tag="L3M60_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6227088.1"
                     /translation="MEKFASFLERKIMPVAAKISNQRHMRAVRAGIIATLPLTIVGSF
                     FTILLNIPIDAYMDFIAPFKATLDIPFRYTVGFLSLYATFGIASSLAKSYDLDTTGVG
                     MLAVMAFLVSTIVPTQVVESVGGVIEAGRWMDMAKLNSPSLFGAIVTSLITVEIYRFM
                     KEKNITIKMPAGVPVEVSNSFTALLPTAVILMLFWVIRHILGFDISAALSAMLSPLKG
                     FLSGNSLGGGLLTVFLITLFWVLGIHGPAIMGPVIRPIWDMSIAENITAFTEGVSSHA
                     MPNIFTEQFLQWYVWIGGAGATLALVVMFMFSKSQYLKSLGRLGFLPGLFNINEPIIF
                     GAPIVMNPILGIPFIIGPIIMTILSYVLTITGIIPMMVARLPFAMPAPIAAVMSTNWS
                     ILAGILVLINFVISFAVYYPFFKVFEKQQLQREQEEMSENL"
     gene            complement(13405..14106)
                     /locus_tag="L3M60_002434"
     CDS             complement(13405..14106)
                     /locus_tag="L3M60_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6227089.1"
                     /translation="MLRNQPLYIQVVDILKQKILNNEYKIDENIPTERELEQEFGVSK
                     ITIRKAIEILESEGYVEKKSGKGTKVISNSLFNKLSKAESFSSILLSKGHKLSKETIS
                     IEKIINDTSSQVFSIFGEKCSKITRMYYLDSKPYIFFTHYIPFDENLTVESFNNESSL
                     YMYLYRRNINITRFEDEFFIEESELEVSKALNLDKDILLGRKRKTYDESDNIVELSFA
                     RYNTEVQNYVIKYEI"
BASE COUNT         4464 a         2692 c         1560 g         5410 t
ORIGIN      
        1 actttaatat tttaaactaa gaaagttaaa gtttatctca tataataaaa tgatacttac
       61 ctaattcatt cttttaagtg tctcatctaa tgaaatacac tctgcatatc ttccattcca
      121 gataaattca ttgtaatatt tgtatgattg ttctgctgac atgaatgaat tgtcaatagt
      181 tgtatttgca taagatggaa ctattatatt aaacccatgt tcaaacccac attttatagt
      241 agcgtcaatg caataatctg tttgaagtcc agcaataatt atatcttttt cattcttatt
      301 tgttaagtat tctaatagac cagtttcctt aaaagcactg tttacatctt tatcaaatat
      361 tttttcttca tcacatggct taaattcttc atatatttca aaaccatgtt tacccttggt
      421 taattcacaa tcaattccat catcatgtcg tacatatatt acttctatat atttttttct
      481 agctgtatca attagtttta ctaaatttac tacaaattca ttaaaattat atagctcttc
      541 atttgttatt aatttttgtg tatcaacaat caataaaacc atatctattc ctcctgttct
      601 taactaatct tttccctcat aaaattttag tatgtctctg aacattccta aaaatttagg
      661 tacttgtcca attacataca ttatattttc ctacttatta gataaattat ttatgctcat
      721 taaacgtatt tctattaaca actacagcat tatctaaact ttgaagtatc aaagtcagta
      781 tagtatttac aacctttaga atcactcttt ttattttata atccaagtgt aaacttgtta
      841 atataataca ttataaatac tatattatct acatatttct aacaatattt cttttacttt
      901 aagatgtcat taaacacatt taatttttat tttaaatgat ttcctagaca taaaaatatt
      961 cccttcatga taacagtttt attgtttcaa ctatctgaca tcataaaagg aacatcttca
     1021 tatttaaaca cattctaaat attcttttaa caaatatatc aaattattct aatgctattg
     1081 tctcatattt attaatctta gtttttataa atttatttta aaattatata ttcacctact
     1141 tttaaagcta caacaagtct ttttctctag taatagcaag tacctcctct acttaccata
     1201 aactatatta tctcattgga atatatccac gatactgtga gtatgcatcc atagttgcta
     1261 tatcacctaa aaatactgat gcgatattga attgtggttt taaatatggg aactcctctt
     1321 cttgtgccca ttctggcttt ttaataaacg attttgcctc ttcaaccaca gctttatacc
     1381 actcttcttc cccttcaaac catagttctg ctacacggtc aaaagcagtt gctccataat
     1441 taatcattat tttactactg acaaaacgat taacatagca acagtttgta aagtatggaa
     1501 ctacctcatc atagaaccat ttttctcctt cttctttgga aacaccatct ggatacttaa
     1561 tcataaattg ccaacgatag tttggtccat cttgtacagt acgtcctttt cctctaaaat
     1621 cttcttccca gtttattgga acaaatgcaa atataaatgg tggacatccg ttatctccac
     1681 ccactgcacg tcctgcatct ccactttctg cattttcatg aaccctttta ttttctatat
     1741 ctggtataca tccttgccaa cgtagaacat ccataggcat atattctgtc ataattctat
     1801 ttgccatttc aggcatatgc tcatctacaa gccaataatg ttctgttagc tgcatcttac
     1861 gtgcaccaaa acgctcacct tcatttggtg taggatatgc ttgataaaag gcatatttag
     1921 tacaatatgg tccaaaagtg ctaatactat ctggaatatg gtgacgatat aaccagtcca
     1981 ttaacttagg tctatattct tccttggcaa gtcctacata tattaaactt ctcattattt
     2041 taaattcaaa acccataacc attctcctat aaccaaagta tattcacatg ctttcaattt
     2101 aactttttct tatccttact caaaatatat cttgtaaaca aatctacacc tacacccaca
     2161 taccaaattc ctactgccat agctatagca atttgaatac aaatatcttg taaattaata
     2221 atattttcac ctgtcataat agttagacct atagcccaac cacaaagcca tgatggtagg
     2281 aaaattattt tttcaaatga ggatgcaatt acaactgcaa aaaaacctaa tatactaagt
     2341 gttaaaaata attccaggtt ttgattgaag ttcatatatt ccataattaa aatggctaac
     2401 actgcccata aatccccaca ccaaaaacca aacattattt ttaatccatc tttttgctta
     2461 ttaccattag ttacatataa tcctgcacag attagtgcta cagctccagt ttttattcct
     2521 atataaggtg ctaatactgc ccataaagga ggtagtaaag caatacccat ggacagtgtt
     2581 aatatttctt ttttctttct atccatttcc tatattgtaa ctagtagctt ccctagtttg
     2641 ccttatctct aatagcttaa ttgctatttg gtttgcaata atagatacta taaaataact
     2701 taagtatagt ggtataatta tagcaataaa ttctggagtt aatctaacat tgtaatgtgc
     2761 aaatactaaa caaataacta ctaaaataat ttcaatctgt ttaaagctcg gcttttcatt
     2821 catacaataa gcctcctata acatccctta aaaaagagtc ttagtatttt aattattatc
     2881 ttaatgaagc tccagcttgc ttttctttag ttaatggttc tataaaatta taagctgttt
     2941 ttctttcttt aaattctggt gttttacgaa cttctgcata atgaccttcc attttcttaa
     3001 tatcatcacc atgtataaac ttacctggta aagtgtcatg agcatgacct aattgctctt
     3061 ctggaaaact aaatatagtt tttccactta tagtatctaa ttctccaact ataccacata
     3121 gagcacaaat tgccttttta gtacctggct caatgtagaa taatctatta tggcaatgtg
     3181 gacatacacc ttcttctcct tggtatgaag ctttttcaaa atcttttgct gcttcaacta
     3241 tatttaaacc aagttggcga acttgagcaa gctttgtatc atccattact aattcctttg
     3301 cccatgggaa ccaagcgtta tctataactt tccacattgg tattagagct agcatagcat
     3361 gttcacactg aactctcgtc ccccagtcag aaccccctat tcccataaat gatattactt
     3421 tatcattaag cattcttgaa tctggaattt ttcctcctgt agcttctgca acttttgtcc
     3481 ctataacatt atttcctcta tctgctcttg gtccaaaacg atccattata gtatggaata
     3541 atcctgatgc cccttcttca aaaatagggt ctgaaactac tataccatct gcatctttca
     3601 ttttgtcaag caaccattca aaatcatctt ttaatacaca tatactacct tttccagata
     3661 ataaagtctt aacgcacgca caacatccag tacaatgttt aatgttaata gatgacattt
     3721 gaataaattc tacttcagcc cctgcttctt tggcaccttt aagtgcaact ttacacattg
     3781 tgtcattatt tccatttttt gttccaaaag aaatacctag tattttcatt ttcaactctc
     3841 cctctttagt acttatttaa tcttctttca tttttaatat ataaaatcca tcatctaaag
     3901 catctatgat gatatagcct ctatcatcaa caataatatc ttctgttact gccaagcgtg
     3961 gtcctagaaa tcctgggaaa taagactctt ctggtgtttt atttggattt ggtggtataa
     4021 aatatgctag ctctttgata taatatgggt ctgatacatc ataaaccctt aaccctgcat
     4081 ggaaataaca gcaatatact ctatctcctc tttgctctaa ccatgactta ttatccattg
     4141 gttcgtgaag attatgtgga ccaaatggtc ctggttttcc tagtcctgcg acattaaagt
     4201 taggataagg gaaatctttt ggcacttcag gatatggaaa ttgagcaatt aatgttggtt
     4261 ttgttgggtc actaacatca accatatgta tattattcat agcttgaaca tctgtaatat
     4321 tatctggttt aaagaattga aatcgttctc cctcattttg gacaacgact aaatctcttc
     4381 ctggcaatgg taatgctgta tgagttcttg cacctgcaag tctactagaa aatgcaggca
     4441 taaatggcaa ttcacctaag cattttggtc ttgttaaatc ttcaacatct aatataatta
     4501 aaccagctcc tccataacca caatatgctt ttccatctct tacaaatgga ggtccatgaa
     4561 gccatccttt atccataaat tctgggcaat gaggtgctcc tgcatcaaaa gttctatttg
     4621 ggtatccatc tgcatattgg tctggtctcc accatttacc tatttccact ggattagtag
     4681 gatttattat atctatgact ctgtaaacca ggccttcaaa gccaacacaa tcacttgata
     4741 aatgtacata tcttccacca ttgtacatga atctatgaac acccattaca tgctttaaac
     4801 cacaatccca atatcctaaa aacttaggat ttaaagggtc ttcttttaaa ctatatattc
     4861 taattcctgc ttcacactta atatttgcta atttagcctg gtcaacaagt gctcctggcc
     4921 cacttccaca actcattgct actatcatta aatcatctgc tatttgaatt tttggagttg
     4981 ttgttgtagg atactcttta gggtctaaca tttgaaaatg ttttataaat tgtggattat
     5041 aagggtctgt aatatcacta atcattactc catttttttt atttcctcca aaacagcatc
     5101 catataaata gtatttttca tctcttttgt acagagccat ttgaaacatt ccacaagttc
     5161 catccaagtt attgaaggaa caaacttcaa gatttttaat atatccatta tttcctttgc
     5221 cttttgcata aaatttatct tccatttttt ctcccttctt atttgaagta ttatcaattg
     5281 atatactttc attttatgag acaaacttac ttaaatccaa tatcgttttc atgggaatat
     5341 ataagttatt cttattaata aatttataaa atctaacaca aagaaatact taaaacatat
     5401 atttttatac aaatattttc caatattctt taaaagtaaa ttttcttata ctataataag
     5461 ttttaattat atataaataa ggtttacaaa caatcaagtt taaatagggg tgattatttg
     5521 aatactaaac aattagaata ttttatatct gtagcagaaa atctaagttt taccaaaaca
     5581 gcagaaaaat tttatatttc ccaaactgct gttacacagc aaataaaatc tttagaagaa
     5641 caaataaatg taactctatt tacaagaagt aaacgccatg ttgaactaac accagctgga
     5701 aaagtttttt tatctgaggc acgcactata ataaaaaata tcaatgatgc tattgcaaaa
     5761 acacaacaat ttgcacatgg tttttttgga actttgagca ttggaacctt aataggatac
     5821 gaaaaaaaca aacttcaaca atatttaaaa gaattttcta atacataccc taatatatct
     5881 atggatattt gtactaatga gataaccgaa cttttaaact tagtaaaaaa taattctatg
     5941 gaccttgctt ttgttattaa tccagaaaat caaccattaa aagattttga acataaaact
     6001 gtagaaagat attctttggt agctctttta cctcctggtc atccacttta caatgaggat
     6061 tctatcgatt taattgaatt acaacatgat aaatttattt ttgtaaagga aacaggggat
     6121 gagtatggac aaaagtcaat gatacagaat agatatcgtg aagcggggtt tgtacctaat
     6181 gtggtacaac gttcaaataa tcttaatact attgaatcat tggtagcttc taacatgggt
     6241 atatccattt taccttcatt ttgcgttaca gatacaatga tagaacgtga tattgcaata
     6301 gtacctataa aagaagtaga aaatagaatt gaagttgtag ttgtatggaa taaaaaaaat
     6361 acaaatccag cattagaaaa gttcatttct ataatataaa attaattaat cttgactata
     6421 aaaatagcat taaccatttt ttctatggtt aatgctattt tctactattt tattatttaa
     6481 cttttaattt aatattcata atcactaaaa agaactatgt ttaacaaata taagcaagtc
     6541 aaaggtgact ctgaaaaaaa taaaaatttt attctatata aaggtgttat ctcaatatat
     6601 ctattttttc attgttcatc aatctaagta catgacttgt gcttactaaa gaattatccc
     6661 cataagttgt atgagctaac actgaactag caaccccaaa ctcaactata tcttcattac
     6721 tcatattttc caaatatgag tatataattc ctgcgacata tccatcccca gcaccaactc
     6781 tgtcatatat ttctaactta tactccttgc tttcaaaata accatcatca tttgatatat
     6841 atccctttat aaatctatta ttcatatcat caaatcttct catcgttcca gctacaatat
     6901 ctatatcaaa ctctttttta atcaattctg aactagattt tatatctaga ttatctatat
     6961 taagtatttt atttatatct ctgcaactag cgaacagtcc atgacaatat ggaagtattt
     7021 tcttataatc atttactaaa tcatcataag acctttctgg atttagagat tgtctaaaat
     7081 taaagtcaaa gaatactttt atcccctttt cataagactt tctagctagt tctaaagcaa
     7141 tctctttaac tgaatctgta agttgcatag caataccaca aatatggact acatccattc
     7201 cttctagaca tttatcaata tcataatctt gtaatttact ctcgcagaat gaactattta
     7261 acctgtttaa ataagtcact tctgagggtc tttctccata tccactttca agaaagtaaa
     7321 ctcctatatg atttcctcca tatgtgataa agtcatcttt tactccaagc tttcttacat
     7381 ttgcacttga tgctttacct acattattat taggcaactt tgtcaacatg taggtattgt
     7441 atccaaagtt tttaagccca gataatatat ttaaccctgt acctgtaatt atataattta
     7501 gttcattggt ttgtgttaaa aacttataac caggtactga aagtctcatc attatttcac
     7561 caaaagctaa tatattcata aatttatccc aataattctt tcattatttt ataaagtact
     7621 tctatatctt ccacttttgt atcacctgtt tctttatcaa ttattgatgt ataaacatga
     7681 ggtataactt tttctacacc agcatcaagt gcaatttgaa ctatctcttt aaagttatct
     7741 aagtctatac ctcctgttgg ttctagataa aaattatgct ttgcacaagc ttttgcaact
     7801 tctatatatt catcctttgt tttaagccct cccattggga aaaatttaat tgagcttcct
     7861 cccatatctt tcatcatggc tatggctgtt tctatatgaa ctataccatc tttttctttt
     7921 gaacttaatg gacctgtaga tatttttaca aatcctggtt taccagttgg agaaactaat
     7981 ccatttatta tactatcatc ttgaccaagc aatgctctag atgtagctac acctgtaaac
     8041 acttgattta tatgttgtgg ctgaagttct ttagaaatct gagataccat atttgactga
     8101 tttgggtctc cagctccaag gcccacagat attgcattat ctatttcctt tgagtatttt
     8161 tttatatctt caatagctac ttctacagtg ctataattct tggataataa tcctactagt
     8221 atatgcccat cacaagcatc atatacttct ttagcatttt ttatacttcc tgcaagaaca
     8281 ttcaaacaaa ctctattttt ataatattgt ggtgtacaat tcatatttat cccctccatt
     8341 ggtaaaattc aatttattaa atcattataa tgtcacttat tccactctct acttctctat
     8401 tatctctttt attttattat aaattatatc taaatcacct ccagttacac tccttatatc
     8461 aatctcaatc ttaccttcat tagccttata gtccctagtg tatatggcta tctctccacc
     8521 tctaagttca tttataattt gttgtgtaga cttatttatt atatcttcat taaagtcaat
     8581 ttctccacga agtatctctc tacctgctga atctctagtt atactagcac tcactccctt
     8641 tagagtattt atcttatcta taaatggatt tagcttgtcc tccatttctt ttaaagttac
     8701 tttatcatta cttatatatc tctcaattgc acatgttaga cctacaatat tttcttttcc
     8761 tattttcata gctcttccta taccctttga ttgaagcttt acattatcta tgtatttttt
     8821 cttacctatt actaaccctg agctaggacc ctctatagcc ttagcaccac tgtatatcac
     8881 taaatctgct cctaattcat agtatacatt taaatcttct tctgccgctg catctactat
     8941 caaaggtaaa ttatattctt tagcaacttg tactgcatct aacacactta acatactctt
     9001 ttgaacacaa tgatgagatt taatgtacat aattgctgct gtattttcat ttatctctct
     9061 tattatatgt tccttgttac acatattagc ataaccagcc tcaacaactt ctcctcctcc
     9121 aagtcttatc attacttcta ctggaactcc ataatcaaca ttgtgcccct ttggaattat
     9181 tatttctctt ttaacttgct tcttaggatt atttacatta taaactagac tcatatcatc
     9241 ttttgctatc attgccccca cactctgtgc aatcccagca gatgcactag aaactattag
     9301 tgcactttca caccctaata aatttgctat atattcacct gttttattta caagttcttc
     9361 tattataaag aaatttttac acccttcacc tattgcttca ataacttctt tatctactgt
     9421 tgatactcct agtatagtca tcttacctga agcatttata acacgtctta cagagtattt
     9481 ttcaaatatg ctactcatac aatagtttct cccttctact cattctctcg tatagactta
     9541 cctatattta aatattctcc attgactact actgctcttg gttttataga tatacttgta
     9601 attactgact tattcaaaga atcttgaagt tctttttctt catcaaccac atcaaatata
     9661 gttaaatcag catcatatcc ttcttttatt aaacctttat tttttaaact tattgctttt
     9721 gctgcattct tagtaacttt atttattata tcttcaagtg agtaacccat atagataaac
     9781 ttttccattg ttgtagatag attatatact ggaccattta ttctgttttt aatatatata
     9841 tctgtactta tagtatctgg aaaaatctct gcatccttag atttcattgc aacatccata
     9901 ctaaaacttt cagttccatg acctacatca aggattatac ctctttctct agcttctata
     9961 atttcctttt taacctcatt gcctctaagt attccatttg gctttccatt atatatatga
    10021 gttaatatat ctcctttttc catatagtcg aatatatcct ctatcattgg aggagaactc
    10081 ccaaagtgaa ccatcatggg taagttaagt tcgtttttta ttcttttagc tacttttaaa
    10141 ggtttaatat cgtttgaaat tacgacactt ttactcattc tagctttgat tccaacaaca
    10201 aaatctttgt attttttaac cgcattttta agttcatctt catcaatatt catcatattt
    10261 gaaagttcat cttgagatgt tattccctgc ttagcaatat ttattaaagc atatacatta
    10321 gttttatact ttttagtatc ttcataaaat ttacctatat ctaaagctcc tgttgttcct
    10381 gcatctacta cagtagtaac tccacttttt attccaacct catctggata atccttataa
    10441 aggtctagtc tttcgtagca gtgcgtatgt atatctatcc aacccgcact aataaatttc
    10501 tctccatcca gatttataat ttggtttgcc tgatacttag aatcatctat ctgatttttt
    10561 acttctatta ttttatcgtc taatatagct atatctatta tacttttatc tactgttttt
    10621 ccacctttta ttaatatgtc tatcttcact ttactctccc tctaaaataa tcttaaattt
    10681 actccaaggt tcaataaagt ctaaaagtat tatttcgtct tcttttattc tacctacctt
    10741 attttttctt tcatctgtac aatcttgaag tattaactgt aattcccctt tatactttcc
    10801 aaagtcatca ttaccaatta ctacatctcc tcttttaaag atgttagtat tatcatgctt
    10861 agggaaattt tctttagcat atttttttct tacttctgta gaccttgcta cctgattagt
    10921 tatatcccct cttctaaagt gttgctcatt taccattatt gttttttcta tttcagaagt
    10981 tttttcctta gctactacat taaatacaac ttggtatcta tctacttcag acattctttt
    11041 taattcatct tcacttgcat atgcatttcc tacaattaca tcatcaatca atcctgttgc
    11101 aaataagtgt ttagttgatg tagcaatatc taagtttcta tgcatttcaa gcgtacacaa
    11161 tccatcattt atattccaag gtccgattgt tgcatatttt gaattaacaa aagcagcagt
    11221 tcttattcca tactttttaa atctatttga acatcttaca aaaaaatcat atggcaagcc
    11281 tgtacctctt tgaggataaa aattgtgaca gccatatata ttgtttacat ttggcttgta
    11341 agtaattata ttatccaagt atgctacatc attactcata tttagttcca cttttaaatc
    11401 gtatggattg aaagaaatca tagcttcttt acttccatca tatccatagt caagtcttat
    11461 tccactagca tttaactcac taaagaatgt taaatcatca taagatattt ctaatatatc
    11521 aaatatgttt ggagctatat caaggattac ttcaaatcct aactctcttg cataggttat
    11581 aatctcttta aaatgttttt taacttcttc cttaggtctt gtaattgaaa gcatacacat
    11641 aaatattctt ttgaatccgt attttgaagc catttttata tatgccttat catcttccat
    11701 tttactatgc tctggatata ctgatatccc taatcgtctt tccatatttc tcctcctcaa
    11761 atcacattat ttaaaatata taaaattaaa ggcgaaggtc attcctccta ccttcacctt
    11821 taataactta taagcttttt tctatatttt atatagtaaa aaacttctat gtatatgtat
    11881 ttttataaat tttcgctcat ttcttcttgt tctctttgta attgttgctt ttcaaaaact
    11941 ttgaaaaatg gataataaac agcaaatgat atcacaaagt tgattaatac taagatacct
    12001 gctagtatac tccagttagt actcattact gctgctatcg gtgctggcat tgcaaacggt
    12061 aatcttgcta ccatcatagg tataatccct gtaatagtaa gaacatatga tagaattgtc
    12121 attattatag gtccaattat aaatggaatc cctagtattg gattcataac tattggcgct
    12181 ccaaatatta taggttcgtt tatgttgaat aaccctggta aaaaccctaa tctacctaaa
    12241 ctctttagat attgcgactt agaaaacata aacattacaa ctaatgcaag agtagctcct
    12301 gctccaccta tccatacata ccactgtaaa aattgttctg taaatatgtt tggcattgca
    12361 tgagatgata ctccttctgt aaatgcagtt atattttctg ctatagacat atcccatata
    12421 ggtcttatta ctggtcccat tatagctgga ccatgtattc caagtaccca aaataaagtt
    12481 attaggaata ctgtcagtaa cccaccacct aaactatttc ctgataagaa tccttttaaa
    12541 ggtgataaca tagcacttag tgcagcactt atatcaaacc ctagaatatg tcttattacc
    12601 cagaataaca ttaagattac tgctgttggt aataatgcag taaatgaatt tgaaacctct
    12661 actggaactc ctgctggcat cttaattgta atattctttt ccttcataaa acgatatatt
    12721 tcaactgtaa ttaatgatgt tacaatagct ccaaataatg atggcgaatt aagttttgcc
    12781 atatccatcc atctaccagc ttctataacg ccacctactg attcaactac ttgagttgga
    12841 actattgtag agactaaaaa tgccataact gcaagcatac caacacctgt cgtgtctaaa
    12901 tcatagcttt tagcaagtga tgatgctatc ccaaaagtag catatagaga taaaaatcct
    12961 acagtatatc taaatggtat gtctaaggtt gctttaaatg gagctataaa atccatataa
    13021 gcatcaatag gtatgtttaa taatattgtg aaaaacgacc ccactattgt tagtggtaat
    13081 gttgctatta tacctgctct tacagctctc atatgtctct gattacttat cttcgctgct
    13141 acaggcataa tcttgcgctc taaaaaactt gcgaattttt ccatagttat ccccctgtat
    13201 tattgatatt atcctttatt tataatgcaa taacgatttc ataatacaaa ctaattttta
    13261 tgtaattgat aatatcactt atttattaaa aatattataa cattataatg tttgtttgta
    13321 aacattataa tgttataata ttatttttta caactaaata aaaatcctga gtatattatc
    13381 attttaataa actcaggatt cttattatat ttcatattta attacataat tttgtacttc
    13441 tgtattatat cttgcaaatg ataactcaac tatattgtca ctctcatcat aggtctttct
    13501 ttttcttcct aataatatgt ctttatctag atttagagcc tttgatacct ctagttctga
    13561 ttcttctata aaaaattcat cttcaaatct agttatattt atatttctcc tatataaata
    13621 catatataaa gaactttcat tattaaaact ttcaacagtt agattttcat caaaaggtat
    13681 ataatgagta aaaaatatat atggcttact atccaagtaa tacattctag taatcttgct
    13741 acatttttct ccaaatatac taaatacttg tgaacttgta tcgtttatta ttttttcaat
    13801 tgaaatagtc tctttagata acttatgtcc cttagataaa agtatgcttg aaaaagactc
    13861 cgcttttgat agcttattaa aaaggctatt acttattact tttgtccctt ttccactttt
    13921 tttctctaca tacccttctg attctaatat ctcaatagct tttctaatag taattttact
    13981 aacaccaaac tcttgctcta attccctttc tgttggaata ttttcatcaa ttttatattc
    14041 gttatttaat attttttgct ttaatatatc tacgacttgt atatataatg gttgatttct
    14101 taacatgatt cacctcttaa attata
//
LOCUS       DAFNNB010000098        13970 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0099, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000098 DAFNNB010000000
VERSION     DAFNNB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13970
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0099"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..723
                     /locus_tag="L3M60_002435"
     CDS             <1..723
                     /locus_tag="L3M60_002435"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBN6227090.1"
                     /translation="ASITDLNYDDAVRLANDYAEADDHGVMVQDTAWDGYEEIPAWIM
                     QGYGTMAKEAIEQLKEYGVDRPTHVFVQAGVGSLAGAVQGYVASIYDECPITVVVEAD
                     EADCYYKSAEAGDGKPRFVGGDMPTIMAGLACGEPNTIGFEVLKNYSSAFVSAPDWVS
                     AKGMRILGNPLRGDEKVISGESGAVTTGALVSILESEDLKDLREALKLDENSKVLLIS
                     TEGDTDPDKYRDIVWNGECQSK"
     gene            968..2155
                     /locus_tag="L3M60_002436"
     CDS             968..2155
                     /locus_tag="L3M60_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBN6227091.1"
                     /translation="MLNETRKKQLTEVCQDLIRNASYSGQEENVVKAIEENFALLGFD
                     SWSRDRYGNIVGCIKGNKPGKKILFDGHIDTVPVPDASKWSVPPFEARIVDGKIYGRG
                     TSDMKGQVSAMMAAVSYFAEDTNKDFEGELYVAGVVHEEIFEGVSAREISKAVQPDYV
                     VIGESSELNLKIGQRGRGEIVVETFGKPAHSANPEKGINAVYKMANVIQKIQQLVPPT
                     HPVLGDGILVLTDIKSSPYPGASVVPDYCKATFDRRLLVGETREGVLAPIQALLDEMM
                     KEDPELNAKVSYAVEKADCYTGNTIESERFFPGWLYDEEDEFVQAAYKGLKEAGIDSE
                     ITQYSFCTNGSHYAGEAGIRTIGFGPSKENLAHTIDEYIELDQLFKGAEGYYGILKSV
                     YKK"
     gene            2171..3754
                     /locus_tag="L3M60_002437"
     CDS             2171..3754
                     /locus_tag="L3M60_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HBN6227092.1"
                     /translation="MRTLIKHGLIVDGNKTPAYEGDILIENEKILKISQDLNEEADKV
                     IDAKGRVICPGFIDTHSHSDLVILVNPYNEVKIRQGITTEVLGQDGISMAPLPQEHIS
                     SWRKNLAGLDGESDEIDWKYETTENYLKMMDDNGVGLNETYLVPHGNVRMEAMGLEDR
                     PATKEEIQKMCEITERELKAGAIGLSTGLIYIPCAYSLTEEIIEMCKVVAKYDGVFVV
                     HQRSEADTILTSMEEIIEIGKQSGVKVHFSHFKVCGKANWKYIPQVIELLEKAEKEGI
                     RVSFDQYPYAAGSTMLGVVLPPWAHSGGTDKLIERLSDENERAKMKKDIANGIEGWDN
                     FIEFAGIDQIFVTSVKTEKNKDVIGKSLLEIGKMRGKDPLDATFDLLKEEENAVGMVD
                     FYGLEEHIIGFMKRDEQNVCTDGLLAGKPHPRAYGSFPKILGRYVRELNVLTIEEAVY
                     KMTKKAATSFSIRDRGELKEGYFADIVIFDKDTVSGCDDFINSMQYPTGIDYVIINGN
                     CVVEEGKYNKIKAGKVLKN"
     gene            3782..5110
                     /gene="ssnA"
                     /locus_tag="L3M60_002438"
     CDS             3782..5110
                     /gene="ssnA"
                     /locus_tag="L3M60_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBN6227093.1"
                     /translation="MILVGNGRLISQDSLNPYMEDGCVVINDNLIEDIGTTNEMKAKY
                     PVHEFIDAGKKIIMPGLINSHMHIYSSFARGMAVPGKPSENFLEILNNLWWRLDKQLT
                     LEDTKYSAYATYIECIKNGVTTVYDHHASPNAIEGSLFTISDVAKDLGIRTCLCYEVS
                     DRDGGNTIQQGINENVNFIKYAQKDDTDMIKGMFGLHAAFTLSDDTLNKCASEMAGLD
                     TGYHVHVAEGLADLQYNVDKYGKRVLERFNDFGVLKEKTIAVHCIYISSEEISLLNKT
                     NTFVVHNPESNMGNAVGCSPAIELLKRGVTVGLGTDGYTSDMFESLKVANIIHKHHLK
                     DPRVGFEESQTMLFDNNRKIAKAYYKNDLGVLKKGAYADVIVVDYIPHTPLTENTIGG
                     HTIFGMSGRSVDTTIINGKVLMKDRKLINIDEESILAKSRELSQNLWNRI"
     gene            5255..6607
                     /locus_tag="L3M60_002439"
     CDS             5255..6607
                     /locus_tag="L3M60_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBN6227094.1"
                     /translation="MSQDKSPKIAPVDERLPVSKSWVFSLQHVLAMCAGAVAVPMMIG
                     SAARLDHASIVFLINACLFMAGIGTLIQSVGIGNIVGAKIPVIEGTSFAAVAGMTAIA
                     SSYSDSQVAIQTVFGSVIVAGLFCILLAPVFEKLIRFFPSVVTGTVVMVIGISLFPVG
                     IKWITDNKLEAVEPNSLALALAVLAITLLSFKFLKGIWNSTAILVGIVLGTLLSMAVG
                     MADFSAVNGVGWLNLNVPLRFGMPRFEPSAILSLCLIMLVLMTESVGNMIAIHEMVDK
                     EVTGKNIKRGLFGDGISTALSGIFNSFPITPFGQNTGIVGLTGIKSRFIAVYAGVILL
                     VLGFLPKFAAIIGAIPKPVLGGVGFAMFGMVMVGGVKTLSKVKFDGTKNGVIVAVSVG
                     LSMIPLTNPDFYHNFPTWVQTIFHSGITTGSLSAVILNIFFNELGSKKEKNNKVEEAK
                     "
     gene            6797..7906
                     /locus_tag="L3M60_002440"
     CDS             6797..7906
                     /locus_tag="L3M60_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6227095.1"
                     /translation="MANLKVNLYGMEFLNPIMTAAGPGAKDGALCQEAAKGGAGAICT
                     KTISVLPADVPRPCMAKTNSGFLNTELWSELPKEQWIEEEYKKVKKTGLPVIVSMGYS
                     AEQIAEVAPLVKPYADAVELSTHYVGTDVAPIVNALKAAKAVLDVPVFMKMSPHTDIQ
                     TIAKAVEEAGADGLVMINSFGPCMAIDVNTGYPIMGSKAGYGWLSGAPIRPLAIRCIY
                     EASQVVNIPIIGVGGITCGKDVAEMMMAGASAVQVCTEAILKGPSIYGKIAKELNEFL
                     DENGYADVNEIKGLTHKKMTARGFRTHSVAPVVDDNKCIKCKQCVTSCVYEALEVTDK
                     LNIDADKCFGCGLCVTRCPKGALSICYNQVEAMNE"
     gene            8185..9576
                     /gene="hydA"
                     /locus_tag="L3M60_002441"
     CDS             8185..9576
                     /gene="hydA"
                     /locus_tag="L3M60_002441"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HBN6227096.1"
                     /translation="MGIVLKGGKVVTSEKTYCADVRIEDEKIVDIGLDISKENDEIVD
                     VSDSYVIPGGIDTHTHFDLDTGATKTADNFETGTKAAIVGGTTTILDFATQAKGCSLK
                     EGLEEWHEKAKDKCYSDYGFHMAITDWNDSVCNEMEDMVKEGVSSFKLYMAYKGSLQV
                     DDGVIFEALRKAEEIGGIIGFHCENGDIICELVDKAKAENNLSPKYHQLTRPAILEAE
                     ATSRLMKIAEVANSKVYVVHLSCRESLEEVLKAKQRGVDVIVETCPQYLLLNDGLYDL
                     DGFESAKYVMSPPLRDKENNEILWNAISDNQIDTIGTDHCSFNYRGQKELGINDFSKI
                     PNGSPGVEHRMSLLYTYGVCKNKISINKMVEVTSTNAAKLFGMYPNKGAIEIGSDADI
                     LVLNPNKKSKIEAKKQLQNVDYTPYEGYEVDCQIEHVYLRGKEVVRDGELICNTPSGR
                     YINRRLTKGAREW"
     gene            9570..12131
                     /gene="xdh"
                     /locus_tag="L3M60_002442"
     CDS             9570..12131
                     /gene="xdh"
                     /locus_tag="L3M60_002442"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent xanthine dehydrogenase"
                     /protein_id="HBN6227097.1"
                     /translation="MVEFILNGEKVVAKEGKKLLDFLREDMNITSVKNGCSEGACGTC
                     MVIIDGKATKACVFKTDKLAGKNITTIEGLSERERDVFAYSFASQGAVQCGFCIPGMV
                     ISSKALIDVNNNPTEDDIKKALKGNICRCTGYVKIIKAVDLAAKIFRENIDVPKVNCK
                     GLVGENLPRVDAVGKALGYSEYVDDMRIEGMIYGSAVRAKYPRALVKKIDIEEAKAYP
                     GVVAVLTAKDIPGTIKVGHLKKDWDTLIPEGEITRYLGDGIVLVAAETREALEEAKKL
                     VKIDYEELTPLSTTADALREDAPKIHETGNILVREHLVRGNAEEKIENSKYVVTKRYT
                     TPATEHAFLEPECAVAGPYEDDGVIIYSTDQGVFATQHECADMLGLPYEKVRVINKMV
                     GGGFGGKEDMSVQHHAALLAYHTKRFVKVQLTRKESIIVHPKRHATEMEFTTACDENG
                     YLTGMKATIISDTGAYASLGGPVLQRLCTHAAGPYNYQDIDITGTAVYTNNPPGGAFR
                     GFGVTQSCFATECNLNLLAEMVGISPWEIRYKNAIRPGQVLPNGQFADEGTAIAETLE
                     AVKEEYEKHPRAGIACAMKNSGVGVGIPDVGRCRLVIKDGKVHIRTSASCIGQGLGTV
                     VTQVVCETANLLPENIVHEVPDTHLTPDSGNTTASRQTVFTGEATKVASSKLKEALDS
                     GKTLLDLEGEEFYGEYESITDKMGCDKENPVSHVAYGYATQVVMLDENGRLEKVVAAH
                     DVGRAINPISLEGQIEGGVVMGLGYALTEDYPVVKSVPTAKFGTLGLFKSTDIPEIQA
                     LIVEKNKDLLAYGAKGVGEICTVPTAPAVQNAYYVFDKEFRTELPLQNTPYSKKK"
     gene            12283..13629
                     /gene="rpoN"
                     /locus_tag="L3M60_002443"
     CDS             12283..13629
                     /gene="rpoN"
                     /locus_tag="L3M60_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBN6227098.1"
                     /translation="MNFNNSLELTQSQKLIMTTQLKQSLSILNMSKLELEEEIKREAE
                     NNPLVEVEKSGEINWEEYIKDMDRSRRLDRTEISYNPDNEVNLENLVKYSSNLYEDLK
                     FQISLYKLTDKELEVCEYIIDSLDEDGYLRTEEKVIVDTLKIDEELFEKCLISVQQLE
                     PSGVGARSLSECLMIQMASLGIYNEILEEIVTKDLNLIANNKYKEISKKYNMSLQKCV
                     ELINIIKTLDPKPGRTCSVEKSVYIQPDVTVEKIDDEFIVYLNEKDSYQIRINSYYKD
                     ILKNSQSDESAKEFIKERLNSATGLMKNIESRKTTVLKIAEEIVKAQDEFLRKGTKYI
                     KPLKMKDIAEKLEFHESTISRGVNGKYMLTPFGVYEFRYFFSSAIETENNEMASSTSI
                     KKIIKETIKDENKKKPLSDDHISKLLKEKGINVARRTVAKYREELGIPSSSKRKEY"
BASE COUNT         5573 a         1616 c         2863 g         3918 t
ORIGIN      
        1 gctagtataa cagacttaaa ctatgatgat gcagtaagat tagctaatga ttatgcagaa
       61 gctgatgacc atggagtaat ggttcaagat acagcatggg acggatatga agaaatacca
      121 gcatggataa tgcaaggata tggaactatg gctaaagaag ctatagagca gttaaaagaa
      181 tatggagtag atagaccaac acacgtattt gttcaagcag gagttggttc acttgctggt
      241 gcagttcaag ggtatgttgc ttcaatatat gatgaatgcc caataacagt agtagtagag
      301 gctgatgagg ctgattgcta ctataaatca gcagaagctg gagatggaaa accaagattt
      361 gttggaggag atatgccaac tataatggca gggcttgctt gtggagaacc aaacactata
      421 ggttttgaag tgttaaaaaa ttattcatca gcattcgttt cagcaccaga ctgggtatct
      481 gctaaaggta tgagaatatt aggaaaccct ctaagaggag atgagaaagt aatctctgga
      541 gaatctggag cagttacaac tggagcatta gtttcaatcc ttgaaagtga agatttaaaa
      601 gacttaagag aagctttgaa actagatgag aactctaaag tattattaat aagtacagaa
      661 ggagatactg accctgataa atatagagat atagtttgga atggtgaatg ccaaagtaaa
      721 taaaagactt aaggaaaaga aacatttaac aaataaaaga aaaatttaag caaatgaaaa
      781 actaaagcaa atgaaaaatt caaaataaaa actaaataaa attaaaataa agtctaaata
      841 aaaatttaat aaataaaata taaaggaata aaaaatcaat agattaagtg aaatgctaat
      901 aaattaaaaa agtgaaatat caaaataaaa cgtcaacaaa ttaaaaatta tatgggggaa
      961 tatcaaaatg ttaaatgaaa caagaaaaaa acaattaact gaagtatgcc aagacttaat
     1021 aagaaatgct agttattcag gtcaagaaga aaacgtagta aaagctatag aagagaattt
     1081 tgctttacta ggttttgata gttggtcaag agatagatat ggaaatatag taggatgtat
     1141 aaaaggaaat aaaccaggta aaaaaatatt atttgacggg catatagata cagttccagt
     1201 tccagatgca agtaaatgga gtgttcctcc ttttgaagcg agaatagtag atgggaaaat
     1261 atatggtaga ggaacttctg atatgaaggg gcaagtaagt gctatgatgg ctgctgtttc
     1321 atactttgca gaagatacta ataaagactt tgaaggtgaa ttgtacgtag caggtgttgt
     1381 tcatgaagaa atatttgaag gagtttcagc tagagagata agtaaagctg ttcaacctga
     1441 ttatgttgta ataggtgaat cttcagaatt aaacttaaag ataggtcaaa gaggtagagg
     1501 agaaatagta gtagaaactt ttggtaaacc agctcactct gctaatccag aaaaaggtat
     1561 aaatgcagtt tataaaatgg ctaatgtaat ccaaaagata caacaattag tacctccaac
     1621 tcatccagtt ttaggtgatg gaatattagt tttaacagat ataaaatctt ctccatatcc
     1681 aggagcatca gtagtgccag attattgcaa agctactttt gacagaagat tattagttgg
     1741 agaaacaaga gaaggagtat tagctccaat acaagcttta ttagatgaaa tgatgaaaga
     1801 agacccagaa ttaaatgcaa aagtttctta tgcagttgaa aaagctgatt gttacacagg
     1861 aaatactata gaaagtgaaa gattcttccc aggttggtta tatgacgaag aagatgaatt
     1921 tgttcaagca gcgtataagg gactaaaaga agctggaata gattcagaaa taactcaata
     1981 ttcattctgt acaaatggaa gtcactatgc aggtgaggct ggaataagaa ctataggatt
     2041 tggaccttct aaggaaaatc ttgctcatac aatagatgag tatatagaat tagaccaatt
     2101 attcaaaggt gcagaaggat attatggtat attaaaatca gtttataaaa aataattagg
     2161 agatggtttt gtgagaactt taataaaaca tgggcttata gtagatggaa ataaaacacc
     2221 agcatatgaa ggggatattt taatagaaaa cgagaaaata ttaaaaatat cacaggattt
     2281 aaacgaagaa gctgataaag ttatagatgc aaagggtaga gttatatgtc caggtttcat
     2341 agatactcat agccattcag acctagttat tttagttaat ccatataatg aggtaaagat
     2401 tagacaggga attacaacag aagtgttggg ccaagatggt atatctatgg cccctctacc
     2461 tcaagagcat atatcttctt ggagaaaaaa tttagcaggt ctagatggtg aaagtgatga
     2521 aattgattgg aaatatgaaa caacagagaa ttatttaaaa atgatggatg ataacggggt
     2581 aggtttaaat gaaacttatt tagtacctca tggaaacgtt agaatggaag caatgggact
     2641 tgaagataga cctgctacta aagaagaaat tcaaaaaatg tgtgaaatca cagagagaga
     2701 attaaaagca ggagctatag gattgtctac tggacttata tatatacctt gtgcatactc
     2761 attgactgaa gaaattatag agatgtgtaa ggtagtagca aagtatgacg gagtatttgt
     2821 agttcaccaa agaagtgaag cagatacaat tttaacatca atggaagaaa tcatagaaat
     2881 tggaaaacaa tcaggagtaa aagttcattt ttcacatttt aaggtttgtg gtaaagcaaa
     2941 ctggaagtat ataccacaag ttatagagct tttagaaaaa gcagaaaagg aaggcataag
     3001 agtatctttt gaccaatatc catatgcagc aggaagtaca atgcttggag tagttttacc
     3061 tccttgggca cattctggag gaactgataa gttaatagaa agattaagtg acgaaaatga
     3121 aagagcaaaa atgaaaaaag atatagcaaa tggaatagaa ggttgggata attttataga
     3181 atttgctgga atagaccaaa tatttgtaac aagcgtaaag acagaaaaga ataaggatgt
     3241 aattggtaag agtctattag aaattggaaa aatgagagga aaagacccat tagatgcaac
     3301 gtttgattta ttaaaagaag aagaaaatgc agttggaatg gtagatttct atggattaga
     3361 agaacatata ataggattta tgaaaagaga cgagcaaaat gtgtgtacag atggtctttt
     3421 agcaggtaaa ccacatccaa gagcttatgg ttctttccct aaaatactag gaagatatgt
     3481 tagagaattg aatgtactaa caatcgaaga agctgtttat aaaatgacta aaaaagcagc
     3541 tacaagcttt agtataagag atagaggcga attaaaagaa ggatattttg cagatatagt
     3601 tatatttgac aaggatacag ttagtggttg tgatgatttt atcaactcta tgcagtaccc
     3661 tacaggtata gattatgtaa ttataaatgg aaattgtgtt gttgaagaag gaaaatacaa
     3721 taaaattaaa gcaggaaaag tacttaaaaa ttaaatctta gctttaagga ggaataaatt
     3781 tatgatttta gtggggaatg ggaggctaat aagccaagat tctttaaatc cctatatgga
     3841 agatggatgt gtagtaataa atgataatct aattgaagat ataggtacta caaatgaaat
     3901 gaaagctaaa tatccagtac atgaatttat agatgcaggt aagaaaataa ttatgcctgg
     3961 tctaataaat tcacatatgc atatatatag ctcatttgct agaggaatgg cagttccagg
     4021 aaaaccttct gagaattttt tagagatatt aaacaattta tggtggagac ttgataaaca
     4081 gcttacatta gaagatacaa aatacagtgc atatgcaacg tatatagaat gtataaaaaa
     4141 cggtgtaaca acagtttatg accatcatgc aagtccaaat gcaattgaag gcagtttgtt
     4201 tacaatatca gatgttgcaa aggatttggg aattagaaca tgtctttgtt atgaagtatc
     4261 tgatagagat ggtggaaata caatccaaca aggtatcaat gaaaacgtaa actttataaa
     4321 atacgctcaa aaagatgata cagacatgat aaaaggaatg tttgggcttc atgctgcatt
     4381 tacactatct gatgatacat taaataagtg tgcttcagag atggctggtc ttgatacagg
     4441 ttaccatgta catgttgctg aaggactagc agacttacaa tataatgtag ataaatatgg
     4501 aaaaagagtt ttagaaagat tcaatgattt tggagtacta aaagaaaaaa caatagcagt
     4561 tcattgtata tatataagtt cagaagaaat cagtctttta aataaaacaa atacatttgt
     4621 agttcataat ccagaatcta atatgggaaa tgcagttgga tgttctcctg caatagagtt
     4681 attaaaaagg ggtgttacag taggtctagg aactgatgga tatactagcg atatgtttga
     4741 atcattaaaa gttgcaaata taatccacaa acatcattta aaagacccaa gagttggatt
     4801 tgaagaatca caaacaatgt tatttgataa caatagaaaa atagcaaaag cttattataa
     4861 aaatgaccta ggagtactta aaaaaggtgc ttatgcagat gttatagtag ttgattatat
     4921 accacatact ccattgacag aaaacacaat aggaggacac actatttttg gaatgagtgg
     4981 aagaagtgta gatacaacta ttataaatgg aaaagtactt atgaaagata gaaagttaat
     5041 aaacatagat gaagaaagta ttctagcaaa aagtagagaa ttaagtcaaa atctatggaa
     5101 tagaatttaa ataattgata ttaatataca agtaagaaac aagaatttag atgttaaaag
     5161 cagagcttaa acaaaaatag gaatttaaat gaagtatatg aataaaaaat atacggaata
     5221 gaatattaaa ttaaatatag aagggagacc caaaatgtct caagataaaa gccctaaaat
     5281 agctccagta gatgaaaggt tacctgtatc caaatcttgg gtattttcac tacagcacgt
     5341 tttagcaatg tgtgcaggag cagtagcagt tcctatgatg ataggaagtg ctgcacgact
     5401 agatcatgct agtattgttt ttttaataaa tgcttgcttg tttatggcag gtataggaac
     5461 actcatacaa agtgttggta taggaaatat tgtaggtgct aaaataccag taatagaagg
     5521 aactagtttt gccgctgtgg caggtatgac agcaatagcc tcatcatata gtgattcaca
     5581 agttgcaata cagacagtat ttggctcagt aatagtagct ggattgtttt gtatactact
     5641 tgctccagta tttgaaaaac taataagatt tttccctagt gtagttactg gaacagttgt
     5701 tatggtaata ggtatttcac tatttccagt tgggataaaa tggataactg acaacaaatt
     5761 ggaagcggtt gaaccaaact cattagcatt agctttagca gtattggcaa taactttact
     5821 ttcatttaaa ttcttaaaag gaatttggaa tagcacagct atattggtag gtatagtact
     5881 tggtacacta ctttccatgg cagttggaat ggcagatttt agtgcagtaa atggagttgg
     5941 ttggcttaac ttaaatgtac ctttaagatt tggaatgcct agatttgaac catctgcaat
     6001 actatcattg tgtttaatta tgttggtact tatgacggaa tcagttggta atatgatagc
     6061 tattcatgaa atggtagaca aagaagtaac aggcaaaaat attaaaagag gtctatttgg
     6121 agatggaata tctacagcat tatctggaat attcaattca tttccaataa ctccattcgg
     6181 tcaaaataca ggaatagtag gtcttacagg tataaaatca agatttatag cagtttacgc
     6241 aggggttata ttgctagtac ttggattctt accaaaattt gcagcaataa taggagcaat
     6301 acctaagcct gttcttggag gcgtaggatt tgcaatgttt ggaatggtaa tggttggagg
     6361 agtaaaaact ttaagcaaag taaaatttga tggaactaaa aacggggtta tagtagcagt
     6421 tagtgttggt ttgtctatga taccactaac aaatcctgat ttctatcata atttcccaac
     6481 ttgggtacaa acaatattcc acagtggaat aacaacaggt agtttatcag cagtgatttt
     6541 aaacatattc ttcaatgaac taggttctaa aaaagaaaaa aataacaaag ttgaagaagc
     6601 aaagtaaaaa taagacaagt aaaaatctat atttattaaa agtcattaag cattgataaa
     6661 agtaattcag agatattaat aattcaacaa aactatctta tattttaaat aataaaaaag
     6721 taaataaata acgaatacaa ataaaaaaat acaatctaaa tattaaaaat atttaattta
     6781 agggagagat aatgatatgg ctaatttaaa agttaattta tatggaatgg aatttttaaa
     6841 cccaattatg acagcagcag gtcctggagc aaaagatggt gctctctgtc aagaggcagc
     6901 aaaaggtgga gcaggagcta tatgtacaaa aactatatct gtattaccag ctgatgtacc
     6961 aagaccatgt atggcaaaaa ctaatagtgg tttcttaaac acagaattat ggtcagagct
     7021 tccaaaagag caatggatag aagaagaata caaaaaggta aaaaaaacag ggcttccagt
     7081 aatagtaagt atgggatatt cagcagagca aatagctgag gttgctcctt tagtaaaacc
     7141 ttatgcagat gcggttgaat tatctactca ttatgtaggt acagatgttg cgccaatagt
     7201 aaatgcttta aaagctgcaa aagctgtttt agatgtacca gtatttatga aaatgagtcc
     7261 tcatactgac attcaaacta ttgcaaaagc tgttgaagaa gcaggtgcag atgggcttgt
     7321 aatgataaac tcatttggac catgtatggc aatagatgta aatacaggat accctataat
     7381 gggaagtaaa gctggatatg gatggttatc aggtgcacca ataagacctc tagcaataag
     7441 atgtatatac gaagcttctc aagttgtaaa tataccaata atcggtgttg gtggaatcac
     7501 ttgtggaaaa gacgtagctg aaatgatgat ggctggagca agtgctgttc aagtatgtac
     7561 agaagcaatc cttaaaggac cttctatcta tggaaaaatt gctaaagaat taaatgaatt
     7621 tttagatgaa aatggatatg ctgatgttaa tgaaataaaa ggattaactc ataagaaaat
     7681 gacagctaga ggatttagaa ctcattcagt agctccagta gtggatgata acaagtgtat
     7741 caaatgtaag caatgtgtaa caagctgtgt atatgaagct ttagaagtta cagataagtt
     7801 gaacatagat gcagacaagt gttttggatg tggattatgt gtaactagat gtccaaaagg
     7861 agctttaagc atctgctaca atcaagtaga agctatgaat gagtaataat tatatagaat
     7921 tttggtaatt aacttagagt aataggtatt aggataataa aatatatcag ataaacacgc
     7981 attatgcatt aataatacag tctaaagtta ataattttat gatattgatt taactaaata
     8041 ttagtttagc tgaatattaa tttaattagg tattggttta atcttaatat agatttacta
     8101 cgtataagtg aactaactag attactttaa gttaattttt tcatataaaa taaattgata
     8161 cgttataagt aaggtggagt ctatatggga attgttttaa aaggtgggaa agtagtaaca
     8221 agtgaaaaaa cttactgtgc agatgtaaga atagaagatg aaaaaatagt cgatataggt
     8281 cttgatatat caaaagaaaa tgatgaaata gttgatgtta gtgattctta tgttatacca
     8341 ggaggtatag atacacacac tcactttgac ttagatacag gtgctactaa gacagcagat
     8401 aactttgaga caggaacaaa agctgctata gtcggaggaa ctactactat attggatttt
     8461 gctacacagg ctaagggatg ttctttgaaa gaagggttag aagaatggca tgaaaaagct
     8521 aaggataaat gctattcaga ttatgggttt catatggcta taacagactg gaatgactcg
     8581 gtttgtaatg aaatggaaga tatggtgaaa gagggagtat cttcatttaa attgtatatg
     8641 gcatataaag gaagtcttca agttgatgat ggtgtcatat ttgaagcctt aagaaaagca
     8701 gaagagatag gtggaattat tggttttcat tgtgaaaatg gggatataat atgtgaactt
     8761 gtggataaag caaaggcaga aaataattta tctccaaaat atcatcaatt aactagacca
     8821 gctatactgg aagcagaagc tacatcaaga cttatgaaga tagctgaagt tgcaaactct
     8881 aaagtatacg tagttcactt aagttgtaga gaatccttag aggaggttct aaaagctaag
     8941 caaagaggtg tggatgtaat agttgaaact tgtcctcaat accttctatt aaatgatgga
     9001 ttatatgatt tagatggatt tgaaagtgct aagtatgtaa tgtcaccacc tttaagagat
     9061 aaagaaaata atgaaatatt atggaatgct atttcagata atcaaataga tactatagga
     9121 acagaccatt gttcttttaa ttatagggga caaaaggaac ttggaataaa cgatttcagt
     9181 aagataccaa acggttctcc aggtgtagaa cacaggatga gtcttttata cacatatgga
     9241 gtctgtaaaa ataaaatatc tatcaacaag atggtagaag taacatcaac taatgcagca
     9301 aagttgtttg gaatgtatcc taataaagga gccatagaaa taggcagtga tgcagatata
     9361 ttagtcttaa atccaaacaa aaaatctaaa atagaagcaa aaaaacaatt acaaaatgta
     9421 gattatacac cttatgaagg gtatgaagtg gattgtcaaa tagaacatgt ttatttaaga
     9481 ggaaaagaag tagttagaga tggagaatta atctgtaata ctccaagtgg gagatatata
     9541 aataggcgtc taacaaaggg ggctagagaa tggtagaatt tatattaaat ggagagaaag
     9601 ttgttgcaaa agaaggcaaa aaacttttag acttccttag agaagatatg aatataactt
     9661 cagttaaaaa tggatgttca gaaggtgctt gtggaacatg tatggttata attgatggta
     9721 aagcgactaa agcatgtgta tttaaaactg ataaattagc tgggaaaaac ataacaacta
     9781 tagagggatt atcagagaga gaaagagatg tttttgcata ttcttttgct agtcaaggag
     9841 ctgtacaatg tggattttgt attccaggta tggtaattag ttcaaaagca cttatagacg
     9901 ttaataataa tcctactgaa gatgatataa agaaagcttt aaaaggtaac atatgtagat
     9961 gtacagggta tgtaaaaata attaaggctg tcgatttagc agcaaagatt tttagagaaa
    10021 atatagatgt gccaaaagta aactgtaaag gtctagtagg tgaaaatctt cctagagttg
    10081 atgcagtcgg taaagcactt ggatattctg aatatgtaga tgatatgaga attgagggta
    10141 tgatttatgg ttcagcagtt agagctaagt atcctagagc tttagttaaa aaaatagata
    10201 tagaagaagc aaaagcttat ccaggtgttg ttgcagttct aacagctaaa gatattccag
    10261 gaactataaa ggtaggtcac ttaaaaaaag attgggacac tttaatacca gaaggtgaaa
    10321 taactagata cttaggagat ggaatagttt tagtagcagc agaaactaga gaagcgttag
    10381 aagaggccaa aaagctagtt aagatagatt atgaggaatt aacaccatta agtactacag
    10441 cagatgctct tagagaagat gctccaaaga tacatgagac aggtaatata cttgttaggg
    10501 aacatttagt aagaggaaac gctgaagaaa agatagaaaa ttcaaaatat gttgtaacta
    10561 aaagatacac tacacctgct acagaacatg catttttaga gcctgaatgt gcagtagctg
    10621 gtccttatga agatgatgga gtaataatct attcaacaga ccaaggggtg tttgccactc
    10681 aacatgaatg tgcagatatg ttagggttgc catatgagaa ggtaagagtt ataaacaaaa
    10741 tggtaggcgg aggatttggt ggaaaagaag atatgagtgt tcaacaccat gcagctttac
    10801 ttgcatacca tactaagaga tttgtaaaag tacagttaac aagaaaagaa agtattattg
    10861 ttcatccaaa acgacatgcc acagaaatgg aatttacgac agcatgtgat gaaaatggat
    10921 acttaacagg tatgaaggca acgataatat ctgatacagg tgcgtatgcc tctcttggag
    10981 gaccagtgct acaaagatta tgtactcatg cagcaggacc atacaattat caagatattg
    11041 atataacagg aacagcagta tatactaata accctcctgg aggagcattt agaggatttg
    11101 gagttacgca gagttgtttt gcaacagaat gtaatctaaa cttacttgct gaaatggttg
    11161 gaatatcacc ttgggagata agatataaaa atgcaataag acctgggcaa gttttaccaa
    11221 atggtcaatt tgcagatgaa ggaacagcaa tagcagagac actagaagct gtaaaagaag
    11281 agtatgaaaa gcatccacgt gcaggtatag cttgtgccat gaaaaatagt ggagttggag
    11341 ttggtatacc tgatgttgga agatgtagat tggttataaa agatggcaaa gttcacataa
    11401 gaacaagtgc ttcttgtata ggtcaagggc ttggtactgt tgtgactcaa gtagtttgtg
    11461 aaactgctaa cctacttcct gaaaatatag tgcatgaagt tccagatact catttaacac
    11521 cagactctgg taatactacg gcttcaagac agacagtatt tacaggagaa gctactaagg
    11581 ttgcatcaag taagctaaaa gaagctttag actctggtaa aactttactt gatttagagg
    11641 gagaggagtt ttatggagaa tatgaaagta ttacagataa aatgggttgt gacaaagaaa
    11701 atccagtgag tcatgttgct tatggatatg caacacaagt tgtaatgcta gatgaaaatg
    11761 gaagattgga aaaagtagta gcagcacatg acgttggaag agctataaat ccaatcagtt
    11821 tagaggggca aatagaaggt ggagtagtta tgggtcttgg atatgctcta accgaagatt
    11881 acccagtagt taaatctgtc cctacagcta agtttggtac attaggttta tttaaatcta
    11941 cagatatacc agaaatacaa gctttaatag ttgaaaagaa caaagattta ttggcttatg
    12001 gggcaaaagg tgtaggagaa atttgtacag ttcctactgc accagcagta caaaatgcct
    12061 attatgtatt tgataaagaa tttagaactg aactaccact acaaaatact ccatatagta
    12121 aaaaaaagta acaaaaaaaa gaaatattaa ataaaaaaat agatgaaatt gtaaataaaa
    12181 aaaacccttt tgcataaggt atattttaaa tgatagaata tacatataca ggagggtttt
    12241 tcttttataa aataagttta ataaaattga gaggtgagca gaatgaattt taataatagt
    12301 ctagaattaa ctcagtctca gaagctaata atgaccacac aacttaagca atcattatca
    12361 atattaaata tgagtaagtt agaattagaa gaagagatta aaagagaagc agagaataac
    12421 ccattggttg aagttgagaa gagtggggag ataaattggg aagaatatat aaaagatatg
    12481 gataggtcaa gacgtttaga tagaacagag ataagctata atcctgataa tgaagttaat
    12541 ctagaaaatt tagttaaata ttcgtcaaat ttatatgagg atttaaaatt tcagataagc
    12601 ctgtataaac ttacagataa agaattggaa gtctgtgagt atataattga tagtttagat
    12661 gaggatggat atttaagaac tgaggaaaaa gtaattgttg ataccttaaa gatagatgaa
    12721 gaactttttg aaaaatgctt gattagtgtt cagcagttgg aacctagtgg agtaggggca
    12781 agaagtcttt cagaatgtct gatgatacaa atggctagtt tgggaatata taatgaaatt
    12841 ttagaggaaa tcgttacaaa agacttaaat ttaattgcaa ataataaata caaagagata
    12901 agtaaaaagt acaatatgtc attacagaaa tgcgttgaac taataaacat aattaagaca
    12961 ttagacccca aaccaggaag aacttgttca gtagagaaaa gtgtatacat tcaacctgat
    13021 gtaacagtag aaaaaattga tgatgaattt attgtatatt taaatgaaaa agattcttat
    13081 caaataagaa tcaatagtta ttataaagac atattaaaaa attcacagtc tgatgaaagt
    13141 gcaaaagaat ttataaaaga aagactaaat tcagcaacag gacttatgaa aaatatagag
    13201 agtaggaaaa ctacagtact taagattgca gaagagatag taaaagctca agatgaattt
    13261 ttgagaaaag ggacaaaata tataaaacct ttgaaaatga aagacatagc agaaaaatta
    13321 gaatttcatg aatctactat aagtagaggt gtaaatggta aatatatgct aacacctttt
    13381 ggtgtatatg aatttaggta ctttttcagt agtgccattg agactgagaa taatgaaatg
    13441 gcatctagta caagcataaa aaaaattata aaggaaacaa taaaagacga aaataaaaag
    13501 aaacctttaa gtgatgacca tatatcgaag ttactaaaag aaaaaggaat aaatgttgca
    13561 agaagaacag ttgctaaata tcgtgaagaa ttaggaatcc catcttcgag taaaagaaaa
    13621 gaatactaat aagtaaaaga caaatatcag tatagtaagg ggaaaaggct aattaaaaga
    13681 tgaatgtgga ttaggctaaa aattttatga aataaaataa taaaatatgg aaaagtatta
    13741 aaatgctata agatattaaa aaactgggga acattaaaac attaaagaac attagagctc
    13801 taaggaatat tagaatttta aagaagatta aaatttagaa aatataaaaa tactaaaaag
    13861 agctgaaaaa ctggggaaca ttaaaatatt gtaaaatgct aaaaaattag aaaacataat
    13921 taaataagtg cttataaaga atgattaaat taaataagtg tttgtaaaga
//
LOCUS       DAFNNB010000099        13947 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0100, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000099 DAFNNB010000000
VERSION     DAFNNB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0100"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            192..1430
                     /locus_tag="L3M60_002444"
     CDS             192..1430
                     /locus_tag="L3M60_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227099.1"
                     /translation="MDGDILMSKILKYKNEIFLFILVVITYLIITKIFFSKTTIFYDL
                     NNTYDVLLDTDTGVLFNLNVFAISQDNSKHILFSAIISIFAYPIYLFCTSIANPGTTD
                     FNSAYGFGLICLQIITSAMSITLVFNHIKKIKMQRLTLILLTMIMIFSFPQLFMTLNV
                     ERFIYSQFSLIFFIVIANKMKGKNSYLVELAAIPLFGITISNIYLYFFNMIFEFKLKI
                     GKMLKHLLTFILMAYICVVSTKSYESFMNLGNVIQYDTKFISGEPILEKIAMIIERLL
                     YSVFYFPGAGIKKGLFLQNGEVATIPVILTLLALCFCVLSVIENSEKRVPKLCMGIII
                     FNLTLHGIVGYNLVNSSIMAINFSFAVIILLAYFTKALRKNEKNIYNIFLSLLLVTII
                     ISNINGFIEILNIGIKSYPV"
     gene            1459..2346
                     /locus_tag="L3M60_002445"
     CDS             1459..2346
                     /locus_tag="L3M60_002445"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HBN6227100.1"
                     /translation="MKQTNTNIRLLTKISDYIKLMRIKQYIKNFFVFSAIIFSNNILN
                     INLLLNTFIAFVCFCLMSSSVYALNDAIDMDKDKKHPKKCNRPVASGRISKKSAYILF
                     VVLSLISVCLSTVVSFNLSIILSLYLINNILYSLKLKNIILLDVFSISLGFILRVYAG
                     CVAISVSLSNWIILCTFFLSLYLALGKRKKEIETLRGDAVEHRKILEDYDVENLNQMM
                     IVILSSTIVCYALYSTSNPEKPHMIFTTIFVVYGVLRYNYIINTTNESNPTDIILTDN
                     ALKINVILWIITCLIILIF"
     gene            2380..3009
                     /locus_tag="L3M60_002446"
     CDS             2380..3009
                     /locus_tag="L3M60_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HBN6227101.1"
                     /translation="MKKSVLLLDVDYTVINTDSMIDFFIYSLKNKTFKTIIKLPYIIF
                     ILFMYLIRMVPLKKAKEAIFYPIVDFSEEDLKSFFDDCIMKKINESIKKVIYKNKEGY
                     NVIIMITASPYAYMKYFKYYGLADEVIGTEFFYENSRYKNQFIGENCKGIEKVKRIKA
                     VLGKLGIEIDYENSYAYSDSKSDLPMLSLTKNAFLVSKKNGEVIEQINS"
     gene            3014..3478
                     /locus_tag="L3M60_002447"
     CDS             3014..3478
                     /locus_tag="L3M60_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HBN6227102.1"
                     /translation="MSLRIGGNSLGYLHILILSMVPVTELRGAIPIGIAMGLNPIWVY
                     VFSVIGSTLVSIPLILTFRHILQFLRGKKLFKGIADVIDRKINSRMKKLKSVSIIGII
                     LFVGIPLPTTGTWTASAIASILKMRIKDAFMGVFLGNLLSGVIVSALSLHII"
     gene            3728..5095
                     /locus_tag="L3M60_002448"
     CDS             3728..5095
                     /locus_tag="L3M60_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6227103.1"
                     /translation="MENSSTNLGNESVGKLLFKLATPAIIAQIVNVLYNIVDRIFIGR
                     MENGEVAMAGVGVAFPIIIIIMACSYLIGMGGGPLAAIKMGEQNNDEAEKIMSNSFSV
                     LVILAILLTIGFKIGKEPLLWIFGASESTIGYSMDYLNIYLMGTVFVQISMGMNTFIN
                     TQGFATTGMMTVAIGALINIILDPIFIFGFNMGVKGAALATIIAQGVSAIWVLMFLFG
                     KKSILKIKKKYMIPKASIVLPVLGLGISPFIMQSTESLVLISLNSKLQMYGGDLAVGS
                     MAIMSSIMQILMLPNMGVTQGAQPIISYNYGSGQLDRVKKTFKLCLLSCLTYSTILWL
                     LLMIFPTFFVSIFNKNPQLLSMTSWSIKIYFAGAFMFGIQIACQQTFLALGKATISLV
                     LALLRKIVLLIPLIFILPTFFNEKLFAVILAEPVADITAATITAISFFIFYKCFLSKP
                     KAIEE"
     gene            5295..6569
                     /locus_tag="L3M60_002449"
     CDS             5295..6569
                     /locus_tag="L3M60_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HBN6227104.1"
                     /translation="MFIMENDLTMQELLDQQEQVFSKVKVGELTTGKITAVRNDEVQL
                     GLDYGFDGIIPISELNIEKNQYIEDIYHIGDEITAVITKVSQKDGTITLSKLQLDKRN
                     DFAELQKAYDEHRIITVNVEKNIDKGVFANYNTYTFFIPISQLDTKFITDTSKFVGLN
                     LEVYIKELDIRKNRLVASHRDVLQERINKEREERRAQIKAEKEAERARIKQEREEEKA
                     RVKAAKEELFNSLEVGQKRDGKVTKIMPYGAFVDIGGIEGLAHINNLAWTRVESVEDV
                     VSEGQEVEVYVLDVNKETKKIALALKDINNDPWDLIAKEVQIDDVVNAKVLRIIEKGA
                     FVQIKEGVDAYLPISELSDTRVAKVTNVVNIGDEVKVKILDFKPKTKRMLVSIKEATR
                     EPEEDITEYLEVEESLGSIGELFKDKFKDLEV"
     gene            6687..7406
                     /locus_tag="L3M60_002450"
     CDS             6687..7406
                     /locus_tag="L3M60_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HBN6227105.1"
                     /translation="MKKSIMDVHCHTLISGHAHSTFKENVEEASNKNIKYLGISDHGP
                     NMPGGPHPFYFYNLHLLPREVQGVKILRGIEGNIMDYHGNLDVQEDMLQHLDYIIASL
                     HRPCIASGTKEENTNAILKVMDKPKVKIIGHPDDSRYPLDYEPIVKKAKDKNILLEIN
                     NSSLSSNSHRTGTWENVSHMLTLCKTYGVRVILGTDSHICYSIGKFENAEKVLKSVDF
                     PDELVINYHEDEIIGFFDINF"
     gene            7427..7873
                     /locus_tag="L3M60_002451"
     CDS             7427..7873
                     /locus_tag="L3M60_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227106.1"
                     /translation="MSKNKFIYMNYYDNGYFKRGMAIYMVLALIILATIFIYSTTILY
                     ILCIITAVFLFKQGRDFSNKYEGKVYITLDNHSILINNQCIFSIKSKQSQKFNYKIIE
                     KIEVVKNILNIYTDESNYKIRLSALSLEDEKKLLNIIDEKMKKFKA"
     misc_binding    8021..8267
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            8391..10133
                     /gene="leuA"
                     /locus_tag="L3M60_002452"
     CDS             8391..10133
                     /gene="leuA"
                     /locus_tag="L3M60_002452"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBN6227107.1"
                     /translation="MKCGKYKKYDKMQIVNRKWPDNEIFKAPIWCSVDLRDGNQSLPT
                     PMSVNEKVRMFKMLIDTGFKEIEVGFPSASNTEYTFLRKLIDENMIPDDVTIQVLTQS
                     RAHLIEKTFESVKGCKKAIIHLYNSTSVLQRDVVFNMSKQEIIDIAVEGAKLFNEEVK
                     KYPETEFTFEYSPESFTGTEMDYALEICEAVIDVWKPTPQKKVIINLPSTVEMSTPNI
                     YADQIEWFCENVSCRDSIILSLHTHNDRGTCTAASELGLLAGADRLEGTLFGNGERTG
                     NMDIVNVGLNLYTQGIDPELDFSNIDKIREIYEDCTKLIVHDRHPYVGNLVHCAFSGS
                     HQDAIRKGMIAMKNRDNDYWEVPYLPIDPHDIGREYKEIIRINSQSGKGGAVYIMETD
                     YGFMIPKNMHSDFGNVVKIESDRIGEELSSEAIFNLFRKEYIDVESPYKVKKYKIKSM
                     DELNYENDDSNDTNMIEMTARVSYMGNEQRIVGVGNGPVDSFNNALKQCGMKDYKFRY
                     YWEHALEEGSHSRGVAYVGIEHNNEVYFGVSISENINTAAINALMNAINKSYIEEEVK
                     NGDDYDAENLSQTC"
     gene            10090..11367
                     /gene="leuC"
                     /locus_tag="L3M60_002453"
     CDS             10090..11367
                     /gene="leuC"
                     /locus_tag="L3M60_002453"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBN6227108.1"
                     /translation="MGMTMTQKILAKHAKLNEVKKGQLIEADLDIVLGNDITSPVAIR
                     EFEKLGIEDVYDKTKVVMVLDHFTPNKDIKSAEQCKFTRSFAKSKGVVNFFDVGDMGI
                     EHVLLPEKGIVTAGDVIIGADSHTCTYGALGAFSTGVGSTDMGAGMATGKCWFKVPGA
                     IKFVLKNKPNKWISGKDIILHIIGKIGVDGALYKSMEFCGEGVEYLSMDDRFTICNMA
                     IEAGAKNGIFPVDDKTIEYINSHKCTTITKDVNVYQADEDAVYDEVYEIDLAELKETV
                     AFPHLPENTRTVDEIDKDIKIDQVVIGSCTNGRISDLEVVAEIMKGKKVADGVRVMIF
                     PGTQKVYLEAIEKGYITTFIEAGAAVSTPTCGPCLGGHMGILASGEKSISTTNRNFVG
                     RMGHVDSEVYLASPAVAAASAITGKISKPSEII"
     gene            11382..11873
                     /gene="leuD"
                     /locus_tag="L3M60_002454"
     CDS             11382..11873
                     /gene="leuD"
                     /locus_tag="L3M60_002454"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBN6227109.1"
                     /translation="MIANGSVFKFGDNIDTDVIIPARYLNIADYKELATHCMEDIDDK
                     FISKVKKGDIIVATKNFGCGSSREHAPIVIKESGVSCVIASTFARIFFRNSINIGLPI
                     LECEEAANNIEEGDKIEVDFSTGVIKNITKGKEYKAEPFPEFMQNIILNEGLINSIKA
                     NRG"
     gene            11880..12950
                     /gene="leuB"
                     /locus_tag="L3M60_002455"
     CDS             11880..12950
                     /gene="leuB"
                     /locus_tag="L3M60_002455"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBN6227110.1"
                     /translation="MNCNIAVIKGDGVGPEIIDEGIKVLNKICCKFNHRFDCEYVLAG
                     GCAIDETGEPLPNKTVEVCRKNEAVLLGAVGGPKWDKCKGDTRPESGLLKLRESLGLF
                     ANLRPATMYESIKEASPLRTDIVDKGIDFVVVRELTGGIYFGERGRKIIDGIENAYDV
                     ETYNENEIRRIGKRAFEIARNRSKKLISVDKANVLESSRLWRSIMEDLAKEFEDVELS
                     HMYVDNAAMQVVREPSQFDVIVTNNIFGDIISDEASMITGSIGMLPSASLRDDSFGMY
                     EPIHGSAPDIAGKDIVNPIATILSVSMMLRHSFNLEEEAKCIEYAVQSILNKGYRTID
                     IHNGVGTVVGTKEMGELIVNEI"
     gene            13313..13828
                     /locus_tag="L3M60_002456"
     CDS             13313..13828
                     /locus_tag="L3M60_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HBN6227111.1"
                     /translation="MDFLAKYKVLKKQNSSKSCLVCGTQNELGLKADFYELENGELVS
                     ICNTKDWHQSYPGRVHGGMSAAILDETIGRAVSINDDQIWGVTVSLELKYKKPVPTDA
                     TIKVVGRITKENRKLFEGTGEIILPNGDVAVTATGKYMKMPIGQIAEGDFSNEEWFFE
                     ESKEKVEYIEL"
BASE COUNT         5380 a         1445 c         2567 g         4555 t
ORIGIN      
        1 aaattctcac tttatacaac gctaatctat gttatataaa caacgtttga ctgattataa
       61 catcaataaa gcaaatattg tggttgtaaa taaagagcta ttcaattaca ataaaacaag
      121 taagaacatt atgaagaggg ctattaaata taaggaataa attgtaggtg tcagcaaatc
      181 ctatataatg aatggatggt gatatattga tgagtaaaat attaaaatat aaaaacgaga
      241 tatttttatt tatattagtt gtaataactt acttaataat aactaagata tttttttcta
      301 agactactat attttatgat ttaaacaata cctatgatgt attgttagat acagatacag
      361 gggttttatt caatttaaat gtatttgcta taagtcaaga taatagtaag cacatattat
      421 tttcagcaat tatatcaatt tttgcatatc caatatacct attttgtact tcaatagcaa
      481 atcctgggac aacagatttt aatagtgctt atgggtttgg actaatttgt ctacaaataa
      541 taactagtgc tatgtcaata actttagttt ttaatcatat taaaaaaata aaaatgcaga
      601 gactaacttt aattttgctt actatgataa tgatattttc ttttccacaa ctatttatga
      661 ctttaaatgt agagagattc atatatagtc aattttcatt aatatttttc atagttattg
      721 caaacaagat gaaagggaaa aattcttatt tagtagaatt agcagctatt cctctatttg
      781 gaataactat tagtaatata tatttgtatt tttttaatat gatatttgag tttaaattga
      841 aaataggaaa aatgttaaaa catttactta catttatctt aatggcatat atttgtgtgg
      901 tttcaacgaa gtcttatgag agttttatga accttggaaa tgtaatacag tatgatacga
      961 aatttataag tggagaacca attttagaaa agattgcaat gattatagag agactgttgt
     1021 attcggtttt ttattttcct ggagcaggta ttaaaaaagg gttatttttg caaaatggag
     1081 aagttgctac aataccagta attcttacat tattggcttt atgtttttgt gttttatcag
     1141 ttattgaaaa ttcagaaaaa agagttccta agctatgtat gggaataatt atattcaatc
     1201 taactttaca tggtatagta ggatataatc tagtcaattc aagtattatg gctataaatt
     1261 ttagttttgc agttataatc ttattggctt actttacaaa agctcttaga aaaaatgaaa
     1321 aaaatattta taatatattt ctatcattgt tattggttac tataataatc agtaatataa
     1381 atggatttat agaaatttta aacataggga taaaatcata tcctgtttaa aaaataatat
     1441 tgaaagggta gtaagtttat gaaacagacc aatacaaata taaggcttct tactaaaatt
     1501 tcagattata taaagttaat gagaataaag caatatataa agaatttctt tgtattttca
     1561 gctataattt tttcaaacaa catactaaat ataaatttat tattaaatac gtttattgca
     1621 tttgtgtgtt tttgcctaat gtcatcatca gtatatgcgt tgaatgatgc tatagacatg
     1681 gataaggata aaaaacatcc taaaaaatgt aatagaccag tggcaagtgg aagaataagc
     1741 aaaaaaagtg cttatatact atttgtagta ttatctttaa tttcagtctg tttatcaaca
     1801 gttgttagtt ttaatctttc tataattctc agtctttatt tgataaacaa tatattatat
     1861 tctcttaaac taaagaatat aatactatta gatgtgtttt ccatatctct tggttttata
     1921 cttagggttt atgcaggatg tgtggccata agtgttagtt tgtcaaattg gataattcta
     1981 tgtacatttt tcttatctct gtacttagct cttggaaaac gtaaaaaaga aatagaaact
     2041 ttaagaggtg atgcagttga acatagaaaa atactagaag attacgacgt agaaaatcta
     2101 aatcaaatga tgatagttat attaagttct acaattgtat gttatgcact atattctaca
     2161 agcaatccag aaaagcctca tatgatattt acaaccatat ttgtagttta tggtgtactt
     2221 agatataatt atataattaa cactacaaat gaaagtaatc ctactgatat tatcctaaca
     2281 gataatgctt taaaaataaa cgtaatcttg tggataatta catgtttaat aatattgatt
     2341 ttttagtgat atttttagtg ataggatggg gatatactta tgaaaaaaag tgtattatta
     2401 cttgatgtag attatactgt tataaatact gatagcatga tagatttttt tatttactct
     2461 ttaaaaaata aaacttttaa aactattata aagttgccct atattatttt tattctattt
     2521 atgtatttga ttagaatggt accgttgaag aaagcaaaag aggcaatctt ttatccaata
     2581 gtagatttta gtgaagaaga tttaaaaagt ttttttgatg attgcataat gaaaaaaatt
     2641 aatgaatcta taaaaaaggt tatttacaaa aataaagaag gttataatgt tataattatg
     2701 ataactgcat ctccatatgc atatatgaaa tattttaagt attatggtct tgcagatgag
     2761 gttataggca ctgagttttt ttatgaaaat tcaagatata aaaatcaatt cattggagaa
     2821 aactgtaagg gaattgaaaa agtaaaaaga ataaaggctg tattaggtaa gcttggtata
     2881 gagattgatt atgaaaattc atatgcatat tcagactcta agagtgattt accaatgctt
     2941 tcattaacta aaaatgcgtt tttagttagt aaaaagaatg gtgaggttat agagcaaata
     3001 aattcataac ttaatgtcac ttagaatagg agggaatagt ttgggatatt tacacatatt
     3061 aattttatct atggttcctg taacagaatt aaggggagcc attcctatag ggattgcaat
     3121 gggattaaat cctatatggg tttatgtatt tagtgtaata ggttctacat tggtatcgat
     3181 tcccttgata ttgacattta gacatatatt acaattttta agagggaaga aactatttaa
     3241 agggattgct gatgtaattg ataggaaaat aaactcaaga atgaagaaat taaaaagtgt
     3301 ttcgataatt ggtataatac tatttgtggg tataccactg ccaactacag gaacatggac
     3361 tgcatctgct atagcttcca tattaaaaat gagaataaaa gatgctttta tgggagtatt
     3421 tcttggcaat ttgctatcag gagttatagt ttcagctctt tctttacata taatataaaa
     3481 ctatctaagt aatatacttg gatagttttt ttatgagtac atgtgaaaaa tttttaagaa
     3541 caaataaaaa gtgagataaa gtatttaaat tcagttagtt ctaaaatgtt tactatggca
     3601 aaagaaatat agtatagtat tacttgtagg gaaagcactc taaatttgtc tgctgataaa
     3661 gtgaaagttt tatagagaat aaaattttag tgatatattt taattattgg attgaggagg
     3721 ttttaaaatg gaaaattcaa gcactaacct agggaatgag agtgtgggta aattattatt
     3781 taaacttgcc acacctgcta ttatcgcaca aattgtaaat gttttataca acatagtcga
     3841 taggattttt attggtagaa tggaaaatgg agaagttgct atggctggtg ttggagtggc
     3901 atttccaata ataattataa taatggcctg tagttattta attggtatgg gaggaggtcc
     3961 attagctgct ataaaaatgg gggagcaaaa caatgatgag gcagagaaaa taatgagtaa
     4021 cagtttttct gtactggtaa ttttagcaat acttttgact ataggtttta agattggaaa
     4081 agagccttta ctttggatat ttggagcaag tgaatctaca attggatatt caatggatta
     4141 tttaaacatt tatttaatgg gaacagtatt tgttcagatt tcaatgggta tgaatacttt
     4201 tataaatacc caagggtttg ctacaactgg aatgatgaca gttgctatag gcgctttgat
     4261 aaacataata ctcgacccga tttttatatt tggttttaat atgggcgtaa aaggcgctgc
     4321 attagctact ataattgcac aaggagtttc tgctatttgg gtgcttatgt ttctttttgg
     4381 aaagaaaagc atacttaaaa taaaaaagaa gtatatgata ccaaaagcaa gtatcgtctt
     4441 accagtgtta ggcttgggaa tctctccatt tataatgcaa tctacagaaa gtttagtact
     4501 tatatcctta aatagtaaat tacaaatgta tggaggagat ttagcagtag gctcaatggc
     4561 cataatgagc agtataatgc aaattcttat gcttcctaat atgggggtta cacaaggagc
     4621 acaaccaata ataagttaca attatggttc aggtcaactt gatagagtaa aaaagacatt
     4681 taagttatgt ctattatcat gtcttacata ctctactatt ttgtggcttt tattaatgat
     4741 atttcctaca ttttttgtta gtatatttaa taagaatccg cagttactta gtatgactag
     4801 ttggagtata aaaatatatt ttgctggtgc ttttatgttt gggattcaaa tagcttgtca
     4861 acagacattt ttagcacttg gaaaagcaac aatatcttta gttttagctc tacttagaaa
     4921 gattgtactt ttaatacctt taatatttat acttccaaca ttctttaatg aaaaactttt
     4981 tgcagtaatt ttagcagagc cagttgcgga tataacagca gctactataa cagcaatttc
     5041 tttctttata ttttataaat gttttctatc aaaacctaaa gcaatagaag aataagatat
     5101 acaatataaa ttttttatga aaatatttta ttagataaaa attagaattt gtattttaat
     5161 ttaataagat attttctata atgtataaat atatgaagct gaatttattt taaaaattat
     5221 agtaagaaaa atgtaagtat ttgcaatatt aatggtataa tatttacaga tatgataatc
     5281 aataagagga aaggatgttt ataatggaaa atgatttaac aatgcaagaa ttacttgacc
     5341 agcaagagca agtattcagc aaagtgaaag ttggagaatt gacaactgga aaaataactg
     5401 cagtgagaaa tgatgaagta caattaggat tagactatgg gtttgatgga ataataccta
     5461 taagtgaatt gaacatagaa aaaaatcaat atatagaaga tatataccat ataggtgatg
     5521 aaataactgc tgtaataaca aaagtatctc aaaaagatgg gacaataact ttatctaaat
     5581 tacaacttga caaaagaaat gattttgctg agttacaaaa agcttatgat gaacatagaa
     5641 taataactgt aaatgttgaa aagaatatag ataaaggtgt atttgctaat tacaatacat
     5701 atacattttt tataccaatt tctcaacttg atacaaaatt tattacagac acatctaaat
     5761 ttgttggtct taacttagaa gtttatataa aagaattgga tattagaaaa aatagacttg
     5821 tagcatctca tagagatgta ttacaagagc gtataaataa agagagagaa gaaagaagag
     5881 ctcaaattaa ggcggaaaaa gaagctgaaa gagcaagaat taagcaagaa agagaagaag
     5941 aaaaagctcg tgtaaaagca gctaaagaag aattatttaa ctctcttgaa gttggacaga
     6001 aaagagatgg taaggtaaca aagataatgc cttatggtgc atttgtggat attggtggga
     6061 ttgaaggatt agcacatata aacaatttag cttggacaag agttgaaagt gttgaagatg
     6121 ttgtttcaga gggacaagaa gtagaagttt atgtgttgga tgtaaataaa gaaactaaaa
     6181 aaattgcctt ggcattaaaa gacataaata atgacccttg ggatttaata gcaaaagaag
     6241 tacagattga tgatgttgtc aatgctaaag ttcttagaat aatagaaaaa ggggcttttg
     6301 ttcaaattaa agaaggtgta gatgcgtatt taccaatatc tgaattaagt gatactagag
     6361 tggctaaagt tactaatgtt gttaatattg gagatgaggt taaagttaag attcttgatt
     6421 ttaaaccaaa gactaaaaga atgttggttt caataaaaga agcaactaga gagccagaag
     6481 aggatataac agaatactta gaagtagagg aatctttagg aagtattgga gaattattta
     6541 aagataaatt taaagattta gaagtgtaaa ataattgaaa atggggctgt tttaaaacgt
     6601 tacactgtct gttatgacag ctttagtttt gaaatagccc tttagctatt ttcacgaatt
     6661 tatgtaaaac attaggagga aatttaatga aaaagtctat tatggatgta cattgtcaca
     6721 ctctaataag tggacatgca catagtactt tcaaggaaaa tgtcgaagag gcgagtaata
     6781 aaaatataaa atacttagga atatcagacc acggacctaa tatgccagga gggccacatc
     6841 cattttattt ttataatttg catttgctcc cacgagaagt gcaaggtgta aagattctta
     6901 gagggataga aggaaatata atggattatc atggtaattt agatgttcaa gaagatatgt
     6961 tgcaacattt ggattatata atcgcaagtt tgcatagacc ttgtatagct tcaggtacaa
     7021 aagaagaaaa tacaaatgct attttaaaag tcatggataa gcctaaggta aaaataattg
     7081 gacatccaga tgacagtagg tatcctttag attatgaacc tatagtaaag aaagctaaag
     7141 ataaaaatat ccttttagaa ataaataatt catcattaag ttcaaactct catagaactg
     7201 gtacttggga aaatgtaagt catatgttga cattgtgtaa aacatatggt gtaagagtaa
     7261 ttcttggtac tgactctcat atatgttatt ctataggaaa atttgaaaat gcagagaaag
     7321 ttttaaaatc agtagatttt ccagatgaat tagtcataaa ttatcatgaa gatgaaatta
     7381 taggattttt tgatataaat ttttagaata ttgggagtga aaaaatttga gtaaaaataa
     7441 gtttatctat atgaattatt atgacaatgg ttattttaaa agaggaatgg ctatatatat
     7501 ggttttggca cttataattt tagccacaat ttttatatat tcaacgacaa tattatatat
     7561 tttatgtata ataacagcag tttttctatt taagcaaggt cgtgattttt caaataaata
     7621 tgagggaaaa gtgtatatta ctctcgataa tcattcaata ttaataaata atcaatgtat
     7681 attcagtata aaatctaaac aaagccaaaa gtttaattat aagataatag aaaaaataga
     7741 agtggtaaaa aatatattaa atatatacac tgatgaatct aattataaaa ttagattaag
     7801 tgcattgagt ttggaagatg aaaaaaaatt attgaatatc atagatgaaa agatgaaaaa
     7861 gtttaaggca taatatcata ctgttttcaa tagttagcat gttttataga attgcttatc
     7921 aaaaataaaa aatcgagata attatttttt gtgttgacaa tatattttaa tgtgtatata
     7981 ataactatta aatcaaaata tttagaaaat ttaaatgata aaaaaagtga tgaatagaag
     8041 tagtaagaac catgaaactt tcagagagct aaagggtggt gtgatttagc agttgatagt
     8101 tctgaacttg tctaggagct gttttctaag atttaaatct tagaataatc ggagtaattc
     8161 cacgttatag gaaatatagt cattataatt attgtattgg ctattactaa gggagctttt
     8221 tagaaagcta aatagagtgg taccgcgagc aaaacctcgt ctctaatttt attagagatg
     8281 aggttatttt tattctaatg ataaaattga catttattct tatgaattgt gcagtattgc
     8341 gtcgatataa ggtatactga atacaaaaaa acaaaaaagg gggaaataat atgaagtgtg
     8401 gaaagtataa aaaatatgat aaaatgcaaa tagtaaatcg taaatggcca gataatgaaa
     8461 tatttaaggc tccaatttgg tgcagtgttg atttaagaga tggaaatcag tcactaccaa
     8521 cacctatgag tgtaaatgaa aaagtcagaa tgttcaaaat gcttattgat acaggcttta
     8581 aagaaataga ggtaggattt ccatcagcat caaatacaga gtatacattt ttaagaaaat
     8641 taatagatga gaatatgatt ccagatgatg taactataca agtccttaca caatccaggg
     8701 ctcatcttat agaaaagaca tttgaatcag taaagggatg taagaaagca attatacatt
     8761 tatataactc tacatctgtt cttcaaagag atgtggtttt taatatgagt aaacaagaaa
     8821 taatagatat agcagttgaa ggggcaaaat tatttaatga agaagtaaaa aaatatccag
     8881 agacagaatt tacttttgaa tattcaccag agagttttac aggaacagag atggattatg
     8941 cacttgaaat atgtgaggca gtgatagatg tttggaaacc gactccacaa aaaaaagtaa
     9001 taataaatct tccatcaaca gttgaaatgt caacaccaaa catatatgca gaccaaatag
     9061 aatggttttg tgaaaatgta agttgtagag attctattat actaagtttg cacactcaca
     9121 atgatagagg aacttgtaca gctgcaagtg aacttggatt attagctggt gcagataggc
     9181 ttgaaggaac gctatttgga aatggtgaaa gaactgggaa tatggatata gtaaatgttg
     9241 gacttaatct ttacacacaa ggaatagacc cagaactaga ttttagtaat atagataaga
     9301 taagagagat atatgaggac tgtacaaaac ttatagtaca tgatagacat ccatatgtag
     9361 gtaatcttgt acattgtgcc ttttcaggtt cacaccaaga tgctattaga aaaggcatga
     9421 tagcaatgaa aaacagagat aatgattact gggaagttcc gtatttacca atagaccctc
     9481 atgatattgg aagagagtac aaagaaatta tcagaataaa tagccaatct ggaaaaggag
     9541 gagctgttta tataatggaa acagattatg gatttatgat acctaagaat atgcattctg
     9601 attttggaaa tgtagtcaaa atagaatctg atagaatcgg ggaagagctt agtagtgaag
     9661 caatatttaa tttatttagg aaagaatata ttgatgttga gagtccctat aaggttaaga
     9721 aatataaaat aaaatcaatg gatgaattaa actatgaaaa tgatgattct aatgatacta
     9781 acatgattga gatgactgca agagtaagtt atatgggaaa tgaacaaagg attgttggtg
     9841 taggaaatgg acctgtagat tcatttaata atgcactaaa gcaatgtgga atgaaagatt
     9901 ataaatttag atattattgg gaacatgcat tagaggaagg gtcacattca agaggggtag
     9961 cttatgtagg aatagagcac aataatgaag tatattttgg tgtaagtata tcagaaaaca
    10021 taaatacagc agctattaat gcacttatga atgcaataaa taaaagttat attgaggagg
    10081 aagtaaaaaa tggggatgac tatgacgcag aaaatcttag ccaaacatgc taagttaaat
    10141 gaggttaaga aaggtcagtt aattgaagca gatttagaca tagttttagg taatgatata
    10201 acatcaccag tagctattag agaatttgaa aaattaggaa ttgaagatgt ttatgataag
    10261 actaaagtag tgatggtttt agaccatttt acacctaaca aagatataaa gagtgcagaa
    10321 caatgtaaat ttacaagaag ttttgcaaag tcaaaaggag ttgtaaattt ttttgatgta
    10381 ggagatatgg gaatagagca tgttcttctt cctgaaaaag gtatagtaac tgctggagat
    10441 gtaattatag gtgctgattc tcatacttgc acatatggag cattaggagc attttctact
    10501 ggtgtaggtt caactgatat gggagcgggt atggctacag ggaaatgttg gtttaaggtt
    10561 cctggagcaa taaaatttgt tttgaagaat aaaccaaata aatggataag tggtaaagat
    10621 ataatacttc atataatagg taagatagga gttgatggtg cgctttataa gtctatggaa
    10681 ttttgcgggg aaggagtaga atacttatct atggatgata ggtttactat ttgtaacatg
    10741 gcaatagaag caggagctaa aaatggaatt tttccagtag atgataagac tatagaatat
    10801 ataaattcac ataagtgtac aacaataaca aaagatgtaa atgtatatca agctgatgaa
    10861 gatgcagtct atgatgaagt atacgaaata gatttagctg aactaaaaga aaccgtagcc
    10921 tttcctcatc ttcctgaaaa tactagaaca gtagatgaaa ttgataagga tataaagata
    10981 gaccaagttg taataggttc ctgtacaaat ggaagaatat cagaccttga agtagtagct
    11041 gagataatga aaggcaaaaa agtagcagat ggagttagag ttatgatatt tcctggaaca
    11101 caaaaagtat atcttgaagc tatagaaaaa ggatatatta ctacttttat agaagcagga
    11161 gcagcagttt ctacaccaac ttgtggacca tgtctagggg gacatatggg aatacttgca
    11221 tcaggtgaaa agtctatttc aacaacaaat aggaatttcg taggaagaat ggggcatgtt
    11281 gattcagagg tatatcttgc aagcccagct gtagcagcag catcagctat aactggaaaa
    11341 atatcaaagc catcagaaat aatttaatgg aggtaaaatt tatgattgct aacggaagtg
    11401 tttttaaatt cggagataat atagatacgg atgtaataat accagctaga tatttaaata
    11461 tagctgatta taaagaacta gcaacacatt gtatggagga tattgatgat aaatttatct
    11521 ctaaggtaaa aaagggagat attatagttg caactaaaaa ctttggttgt ggttcatcaa
    11581 gagaacatgc tccaattgta ataaaagaaa gtggagtatc ctgtgtgatt gcttctactt
    11641 ttgctagaat attttttaga aattctataa atatagggct tcctatactt gaatgtgaag
    11701 aagctgcaaa taacatagag gaaggggata agatagaagt agatttttcc acaggtgtga
    11761 taaaaaatat aacaaaagga aaagaatata aagcagaacc atttccagag tttatgcaaa
    11821 atattatatt aaatgaaggc ttaattaact caataaaagc aaataggggg tagtggagta
    11881 tgaattgtaa tatagcagtc ataaaaggtg atggtgttgg accggaaatt atagatgaag
    11941 gtataaaggt tctaaataaa atttgttgta aatttaatca tagattcgat tgtgaatatg
    12001 tattagcagg agggtgtgca attgatgaaa ctggagaacc tctacctaat aaaactgttg
    12061 aagtttgtcg aaaaaacgag gctgtattac ttggtgctgt tggaggacca aagtgggata
    12121 aatgtaaggg agatacaaga ccagagtctg gtcttttaaa attaagagag tcattgggtt
    12181 tatttgcaaa cttaagacca gccacaatgt atgaaagtat aaaagaagct tcaccactta
    12241 gaacggatat agttgataaa ggcattgatt ttgttgttgt aagagaatta acagggggca
    12301 tttattttgg agaacgtgga agaaaaatta ttgatggtat tgaaaatgct tatgatgtag
    12361 aaacatataa cgaaaatgaa ataagaagaa ttggtaagag agcttttgaa atagccagaa
    12421 atagaagtaa aaaacttata agtgtagaca aggcaaatgt gcttgagagt tcaagacttt
    12481 ggagaagtat aatggaagat ttagctaaag agtttgaaga tgtagagcta tcacatatgt
    12541 atgttgataa tgctgctatg caagttgtaa gagaacctag ccaatttgat gtaatagtaa
    12601 ctaataatat atttggagat ataatatctg atgaagcatc tatgattacg ggttctatag
    12661 gtatgttacc atcagcatca cttagagacg atagttttgg tatgtatgaa ccaatacatg
    12721 gaagtgcacc tgatatagcc ggaaaagata ttgtaaatcc aattgcaact attctatctg
    12781 taagcatgat gttaagacat agttttaacc tagaagaaga agctaagtgt attgaatatg
    12841 ctgtacaaag tatacttaat aaaggttata gaactattga tatccataat ggtgtgggaa
    12901 ctgttgtagg aacaaaagaa atgggcgagt taatagtaaa tgaaatttag aattatagtt
    12961 taatttgtat aatataattt aaatatataa cttggaagat tgctacaaat ctattatagt
    13021 ataatttgtt ttatgagggg tgtctcaaaa taaactttat tagttaatga ggcactcttt
    13081 tttatattaa gtcaaatata tttttaatat tttaataacg aaaaagaggc ttatatctat
    13141 tttgagtttc tattttgata aagttatttt ttagaaaata atactttaaa ttaaacttat
    13201 catattgaaa tagcctattt ttattattag aagtttgcag ggattttatt aatattatat
    13261 aatatatttt agaattattt aatattaagc gactactaat gtcggttagg agatggattt
    13321 tttggcaaag tataaagttt taaagaagca aaatagtagc aaatcttgtt tagtttgtgg
    13381 aacacaaaat gagttaggac ttaaagcaga tttttatgaa cttgaaaatg gggagcttgt
    13441 atcaatttgt aatacaaagg attggcatca aagttatcct ggaagagttc atggaggtat
    13501 gtctgctgca atattagacg agactattgg aagagctgta agtataaatg atgaccaaat
    13561 atggggagta acagtgtctt tggaattgaa atataagaaa ccagtaccaa cagatgctac
    13621 tataaaagtt gttggaagaa taactaaaga aaacagaaaa ttatttgaag gtacaggaga
    13681 aataattcta ccaaatggag atgtagcagt aacagcaact ggaaagtata tgaaaatgcc
    13741 tattggacaa attgcagaag gtgatttttc taatgaagaa tggttctttg aagaatcgaa
    13801 agaaaaagtg gaatatattg aactttgatt agataaaaat tttatatttt caaacataaa
    13861 ttttatattt ataaaaaata aaattcaaaa atacaataaa aatacaagat aaaaataaat
    13921 tagttatact aaaaagattt atcgtaa
//
LOCUS       DAFNNB010000100        13914 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0101, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000100 DAFNNB010000000
VERSION     DAFNNB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13914
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0101"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            176..2272
                     /locus_tag="L3M60_002457"
     CDS             176..2272
                     /locus_tag="L3M60_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HBN6227112.1"
                     /translation="MIKVVYMRKNKKARKMIIFGFLMCLLIVIIVFLAKFILDGLNKN
                     KPDEVFKQYMSFANKKQYEKMYDLLDEKSKSENKKEDFVLRNKKIYEGIDAHNISIKI
                     NDIKKNKNNDKLINYDSKMDTLAGEILFSNEVLLTRDRQKNYKIKWQSSVIFPELEED
                     NTVRVSKLKGKRGRILDRNGVMIAGQGLASMIGLVPGKMSDNIEDLKKLSTLLNVSVE
                     QIEKKLNASWVKENSMVPIKTIEKIKENPDGTVKEEDKELQESLLSIPGVKISNTEVR
                     VYPFGEKTGHLTGYVQNVNAEILKEKEGKRYNANSIIGKIGLESLLEDRIRGIDGYEI
                     IIADRYGDKKETLVTEPKVDGEDVKLTMDSKLQSKLYDQMKNDKGCAVVMNPKTGEVL
                     SLVSSPSYNPNEFILGMSEDRWNELNKNENKPMYNRFKARLCPGSSFKPVTAGIGITT
                     GKINPNENFGHSGLSWQKDSSWGSYKVTTLKDYGNTVNMENALIYSDNIYFAKAALKI
                     GEDVLSKELLKLGFDESMPFEFGLSSSKFGTDNKFETEIQLADTGYGQGKLLVNPVHM
                     ASIYSAFVNDGDMIKPHLEFKKNLQAEFWKKGVFSKEAVKIIRDDLLQVVESPNGTGH
                     SAMIKGIRIAGKTGTAEIKASKDDVTGTELGWFNAFTVDGNNDKQYLAIAMIEDVKSR
                     GGSHYVMPIVKSVFED"
     gene            complement(2625..4424)
                     /locus_tag="L3M60_002458"
     CDS             complement(2625..4424)
                     /locus_tag="L3M60_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaR1 family beta-lactam sensor/signal
                     transducer"
                     /protein_id="HBN6227113.1"
                     /translation="MYAPFFIRFLLSTLTLSILIVVILLAKKVFKKHISAKYQYNIGF
                     ILLIMLIIPFVPLKYINLGNVFNYLYTFNQLGSNTNRTSMIKNSPNSIINNSNALNDF
                     SVSLNQVNFDILNILLLILWIIGILIMIVITIHCNIKIKSMKKSIQILRDEEIVFIFE
                     KCKKDIGINKDFILCKSPIINTPITFGYFKPHIILPDKSISKLSLKDIKYILLHELQH
                     FKNRDMLINYIMCFFQILYWFNPLVWYAFKEMRIDREIACDISVLKKLDKDNHIEYGQ
                     TLINFVDNISRPSNLTLTSDIGGSKKQIKKRLLKIMDFHNESNTLKFKSILIFTLIAL
                     IVFGNIPKLSIMADINEKYRPTNKQTMYEDLSKYFKGFDGSFVMYDLKSKQYHIYNKE
                     KSKTRISPNSTYKIYSALLGLENGIITRDNSHIPWDGKHHSNISWNEDQDLFSAMENS
                     VNWYFQTLDKKTGIKNIQTYLEQIGYGNYDVSGGISNFWLESSLKISPIEQVELLESF
                     YTNKFNFEDKNIETVKDSLLISKKDGVSLSGKTGTANINGKDVNGWFIGYVEKNENTY
                     FFATNIQSKDYSNGISASKITLSILNDKKIYNP"
     gene            complement(4427..4813)
                     /locus_tag="L3M60_002459"
     CDS             complement(4427..4813)
                     /locus_tag="L3M60_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6227114.1"
                     /translation="MKKLPHISEAEYQVMKIIWKYAPISTTEVIEKLVETSTWSPKTI
                     QTMLLRLVKKGALTYEKNSRVFVYTPLVKEEEYVATESSSFLNRFYNGTLNSMVLNFL
                     ENDKLSEDDIEELREILNKRTTKGDK"
     gene            complement(5184..5810)
                     /locus_tag="L3M60_002460"
     CDS             complement(5184..5810)
                     /locus_tag="L3M60_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HBN6227115.1"
                     /translation="MKINIKSTLNEFIKQNNLFDDNRIISYLPNITIDTDKIKTIMIN
                     EVVPTNTNDDFYSIDKDADYLKTTIPLFNNAGIEVSNINDILDMGIYITNAVKLPKSE
                     YTITRDTIKLHMPILEEEIKLFKNLEVVMLMGDVAKKSFNMITKKHIKKNVIPSISTY
                     KLRNNELYYDNLRVFPSYIMTGGNILIEKSKFEMASEDIKKMFEIING"
     gene            complement(5953..6210)
                     /locus_tag="L3M60_002461"
     CDS             complement(5953..6210)
                     /locus_tag="L3M60_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227116.1"
                     /translation="MILNRTELFRGYSLDRFNEICNILSVNKIKYKYKVKNNTYSKKP
                     FYNEITLGNLGQKEDFSYEYLIYVHKDDYEQAEALINSKLR"
     gene            6988..7692
                     /locus_tag="L3M60_002462"
     CDS             6988..7692
                     /locus_tag="L3M60_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBN6227117.1"
                     /translation="MKDLIIETKNLSKRYGEQMAVNNVSLQIERNSIYGLLGPNGAGK
                     STTLKMLVGLLRPTDGRIIFDRKPWKRESLEKIGSLIESPALYGNLTAEENLLVHTKI
                     LGIPKDKIYEVLEIVNLKNTGKKRASQFSMGMKQRLGIAIALLNDPELLILDEPTNGL
                     DPFGIQELRELIASFPEKGITVILSSHILSEVAQVVDNIGIINGGRLLFQGMPDPNEN
                     LEEFFTDVILKGGQNR"
     gene            7689..8459
                     /locus_tag="L3M60_002463"
     CDS             7689..8459
                     /locus_tag="L3M60_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBN6227118.1"
                     /translation="MNALQSELLKYKRTFMGKLIVFFPVSFAAYAFIMQSTLMQNPLS
                     QINSWAWESLLALIFNWWSMMFLSIGFALFATLVASQEKKAGNYRALRTHNVSPMTIW
                     INKIIAMAVYSFISTLVLIVVTIMTGLILKAGPIPFGQIIGASIVCWVGSLAILPIQL
                     WLATWKGMFLSMGVGALGMIFGVLAASKPFWVAVPWSWAVRMVCPIINIHPNGTILEA
                     GDPLLNTSVIPIGIVVSLVVFIVLTALTAAWFNRRDDK"
     gene            8456..9235
                     /locus_tag="L3M60_002464"
     CDS             8456..9235
                     /locus_tag="L3M60_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBN6227119.1"
                     /translation="MIKLLSSEWLRAKRTAVQGLTLYMPIIFSLCVVAYLTIKSGSTQ
                     EFAFEGFFTIWTVFIIPIGVGILAGFIVQEEELAGNFNGFLCVGISRVRLYLGKFLFL
                     LFCLTICTFIATLILCIGMNIAVPSGATIWLFLSAAGLVVIGTLPLLAIHLWVSFAWG
                     MGASIGISIGGILMAAILGLTSVGAKVWAFVPWTWPVKLGMLPGTYFIKEAGTISTEV
                     FYSKMMQSATVVFIVVAIGLIIFLIGGVIWFKIWEGRKSYE"
     gene            9287..9979
                     /locus_tag="L3M60_002465"
     CDS             9287..9979
                     /locus_tag="L3M60_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227120.1"
                     /translation="MSKILIIDDEIDLVMLLEDELKAKGHEVLIAYDGQAGIELSKNE
                     PDLIILDIMMPKMDGFEVCQAIRDDVLCPIIFLSAKQSETDKIKGLTFGGDDYVTKPF
                     GLRELMARIEANLRREKRSQYINEENKRSTLYFEKLCLYMKERAIKIDGKDIDLTKIE
                     YDIVELLALHAGQVFSREQIYEKVWGCDSEGDSSTVVERVKKIRAKFSAVTPEKEYIS
                     TVWGIGYKWNKM"
     gene            9998..11422
                     /locus_tag="L3M60_002466"
     CDS             9998..11422
                     /locus_tag="L3M60_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227121.1"
                     /translation="MKNQSLATQFRRTFIFIIIASIVATVITYVFAFYLFIYSLNKDI
                     YPPNYYERQVPRIKKYIDEKNIALLSQSNEEGLKRTIRGDGMLYQVVDDNGNILYGNN
                     PKKPFKTKEELFNNFINTTVRKGGYIYTVPIKGTNGKIEGAVILFYQVKMTFANSMGR
                     LTFAVIVIAMFSPFLYVIGFTIWLSKQFVKNINQPLHLLIDASKKIKEKDLDFEIDYY
                     SDNELGKLCSAFSEMKDELKNSLSAQWKMEQERVEMVEALAHDLKSPLSIVLGYTDAL
                     IGNNTDDNEKLHRYLTVIRENTEKSAALVQKMQYTSDLEKSNIQLNPVPINLPEFLVQ
                     KVQDYELQAHQKDVELILKMYGNIQSPIQIDLDRLTRIFDNIISNSLQYTPSGGNISI
                     IAKAEKNCISYEICDSGSGFSSKDLKKALDKFYRGDEARQTKGGHSGLGLYIVKQLVE
                     QLGGSVKIENSKSGGACVKFWHSI"
     gene            complement(11663..>13914)
                     /locus_tag="L3M60_002467"
     CDS             complement(11663..>13914)
                     /locus_tag="L3M60_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227122.1"
                     /translation="KKDDRVYQAGTSITLGSSKIKDSGISVLVKEYDKTGLSNYPKLM
                     KLKSGHLPKDKNEIALDENTLKLIGIKPRLGVTIPMNLDISLLNDTIPPYNYTANFKL
                     SGILEDDYTGYVSGIVNGIVGKGASENLLPKRYILSSLDFKIKQQEKFQEIVNQLAKK
                     INLSHNSIQYNWIYLNALKIQFEKDENSSNSDGISMIILVSLFVALLVLLASGLVIYN
                     ILKISVTKKIKEYGCLRGIGAEPNQIYKIVILQILILCTIAIPIGAVIGIISSKGITG
                     MVTNILNPDILLANDNKEITELIHKNTTAYMFPLVLSTSISLIFSFISALPSAIYASH
                     VSPKIAMAGSTTKIKRKIKREKTIKNFERHLAWLNLKRNKGRTIITILSLFMSITVFV
                     ALSEFSNVLDVSRSVSNLKEGDFSLTNEISGFDKSILDKIDNMKNVNRTSFIKYSEYK
                     QGEIDTDINFENSGEMLKIIGIDEQTLKDLMPSITDSTLEDFKNGSICFIKNPLAIST
                     PGVKTKYTNLKPKDNITINKKQLDIYSTVDKMFFLQGNGWVNGVDIIVYDSVYNTLTN
                     KNKFNQINIYAKDKSDLEQIRLSIEQICENNPGSHWISYIESDKQLKESFKQIELLAW
                     AVILFVGLIGTLNIINTTHTNINTRTNEIGVKRAIGMSNSSLYKMFLWEGVYYGIFAA
                     ILGSIAGYASAIIINMATIEKLDFTNIPITSILQATIISILACIIATLIPLRKVKKMN
                     IIDCIDINL"
BASE COUNT         4889 a         1741 c         2280 g         5004 t
ORIGIN      
        1 aattaaataa taatattatt attgactttt aaaacttaca tgtgtaatat ataaattact
       61 atcaattata ctgcttacat atttttatga gatactaaaa aacataataa aaacatagat
      121 tagttacgtc tataagatta ctttaacctt aaagcttacg tatgtaatat attaaatgat
      181 aaaggtggtt tatatgagaa aaaataaaaa agcaagaaaa atgataattt ttggttttct
      241 aatgtgtcta cttatagtta ttattgtttt tttagcaaaa ttcatactag atggactcaa
      301 taaaaacaaa ccagatgaag tgtttaagca gtatatgtct tttgcgaata aaaaacaata
      361 tgagaaaatg tatgatttat tagatgaaaa gagcaagtct gaaaataaaa aagaagattt
      421 tgttttaaga aataaaaaaa tctatgaagg aattgatgcg cacaatataa gtattaagat
      481 taatgatatt aaaaaaaata aaaacaatga taaattaata aactatgatt ctaagatgga
      541 tactttagca ggtgaaattt tattctcaaa tgaagtacta ttaactagag atagacagaa
      601 aaattataaa ataaaatggc agtcaagcgt catatttcca gaacttgaag aagataacac
      661 tgtaagggtg tcaaagctga aaggtaagag aggacgtatc ttagatagaa atggtgtgat
      721 gattgctggt caaggtcttg catctatgat aggattagtc cctggaaaaa tgagtgataa
      781 tatagaagac ttaaaaaaat tatctacctt actaaatgta tcagttgaac aaattgaaaa
      841 gaaattaaat gcatcttggg taaaagaaaa ttcaatggta cctataaaaa ctattgaaaa
      901 aataaaagaa aatccggatg gtactgtaaa agaagaagat aaggaactac aagagtcgct
      961 tcttagtatt cctggagtta aaatatctaa tacagaggtt agagtatatc catttggtga
     1021 aaaaactggt cacttaactg gttatgttca aaatgtaaat gcagagatac taaaggagaa
     1081 agaaggtaag aggtacaatg ctaatagtat aattggtaag attggtttag aaagtttact
     1141 ggaagacaga atacgtggta ttgatggata tgagattatc atagctgata gatatggtga
     1201 caagaaagaa actttagtaa ctgagccaaa ggtagatgga gaagatgtaa agctaactat
     1261 ggattcaaaa ctacagagta aactatatga ccaaatgaaa aatgataaag gttgtgcagt
     1321 agttatgaat cctaaaacag gagaggtttt atcacttgta agtagtccgt cgtacaatcc
     1381 aaatgagttt atattgggaa tgtcagaaga tagatggaat gaactcaata aaaatgaaaa
     1441 taaaccaatg tacaatagat ttaaagcacg attatgtcct gggtcttctt ttaaaccagt
     1501 tactgctggt attggtataa caacaggaaa gattaatcca aatgaaaatt ttggacacag
     1561 cggtctaagt tggcaaaaag attctagttg gggaagctat aaggtaacca cgttaaaaga
     1621 ttatggaaat actgtaaata tggaaaatgc tttgatttat tctgataata tttattttgc
     1681 taaggcagcc ttaaaaatag gtgaagatgt attgtcaaaa gagttgttga agcttggatt
     1741 tgatgaaagt atgccatttg aatttgggtt atcctcttca aaatttggta ctgacaataa
     1801 atttgagact gaaatacaat tagcagatac tggttatgga cagggtaagc ttcttgtaaa
     1861 tccagtacat atggcatcaa tatattcagc ctttgtaaat gatggagaca tgataaaacc
     1921 tcatcttgaa tttaaaaaga atttacaggc tgaattttgg aagaaaggtg tgttttctaa
     1981 agaagctgta aaaattattc gtgatgacct acttcaagtt gttgaaagtc ctaatggcac
     2041 aggtcatagc gctatgatta aaggaattag aattgctggg aaaactggta cagcagaaat
     2101 taaagcatca aaagatgatg ttacaggtac tgaacttggg tggtttaatg cttttactgt
     2161 agatggaaat aatgataaac aatatctagc aattgctatg attgaagatg taaagagtcg
     2221 tgggggaagt cactatgtta tgcctattgt taaatcagta tttgaagatt aaatttactc
     2281 aatttgaaaa ttaatcttat tcagttgaga ataatatttt agataaattg tttttagact
     2341 ttttagtaaa ttctaaaaca attaaagagg ctgtttcaaa atagagatat gcctcttttt
     2401 cattgtttaa atgataaaaa tatattttac ttaatacaaa aaattaaata gtggcaacaa
     2461 ttgaggatgt gagggagagt tgccactatt tgtatagggt attatattat gtttttaagc
     2521 tagttttact atcttttatg tttatataat aagccttgta tatgaattta atagataatt
     2581 aatatgaaaa ttatgtgaat tttttagaat gtatagtaaa tatattaggg attatagatt
     2641 tttttgtcat ttaatataga taatgttatc ttagatgctg aaataccatt agaataatct
     2701 ttgctttgaa tatttgtagc aaagaaatag gtattttcat ttttttctac atagcctata
     2761 aaccaaccat tcacatcttt tccatttata ttagcagtac ctgttttacc agaaagtgat
     2821 accccatctt tttttgatat taacaatgaa tcttttacag tttctatatt tttatcttca
     2881 aaattaaact tatttgtata aaaactttca agtaattcta cctgttcaat aggtgatatt
     2941 tttaatgaag attctagcca aaagtttgag ataccacctg aaacatcata attaccatat
     3001 ccaatttgct caagataagt ttgtatgttt tttatgcctg tttttttatc tagagtctga
     3061 aagtaccagt ttacggaatt ttccatagca gaaaataaat cttggtcttc attccaagaa
     3121 atattagaat gatgttttcc atcccaaggt atatgtgaat tatctcttgt tattatccca
     3181 ttttccaatc ccaataatgc actataaatc ttataggtag aatttggaga aattcttgtt
     3241 ttactttttt ctttattgta aatatgatat tgcttggatt ttaaatcata cataacaaaa
     3301 cttccatcaa aacctttaaa atacttactt aaatcctcat acatagtttg tttatttgta
     3361 ggtctatatt tttcgttgat atctgccata atagatagtt taggaatatt tccaaataca
     3421 attaaagcta tcaatgtaaa aattaaaatg cttttgaatt ttaaagtatt tgattcatta
     3481 tgaaaatcca ttatcttcaa tagtcgtttt tttatttgtt tttttgaccc accaatatct
     3541 gatgtaagtg taagatttga tggtcttgaa atattatcta caaagtttat tagagtttgt
     3601 ccatattcta tatgattatc tttatcaagc ttttttaaaa ctgaaatatc acatgcaatt
     3661 tcacggtcta tacgcatttc tttaaatgca taccatacaa gaggattaaa ccaatataat
     3721 atttgaaaaa agcacattat ataatttatc agcatatctc tatttttaaa atgttgcaat
     3781 tcatgaagta agatatattt tatatctttt aaagataatt tagatataga cttatctggt
     3841 aatataatgt gtggtttaaa gtatccaaat gtaataggcg tatttataat aggtgatttg
     3901 catagtataa aatccttatt tataccaata tcttttttac acttttcaaa tataaaaact
     3961 atctcctcat ctcttaaaat ttgtattgat tttttcatac tttttatttt aatgttgcaa
     4021 tgaatagtaa taactatcat gattaaaatt ccaattatcc ataatattag caataaaata
     4081 tttaaaatat caaaattaac ttggtttagg gatactgaaa aatcgtttaa tgcatttgaa
     4141 ttgttaatta tagaatttgg actatttttt atcatagaag tcctattagt attgcttcca
     4201 agttgattaa aagtatataa atagttaaat acatttccaa gattaatata tttaagaggg
     4261 acaaatggaa ttattaacat tataagcaat ataaatccaa tattgtattg gtactttgca
     4321 gaaatatgtt ttttaaatac ttttttggca agtaaaatta caacaattaa tattgataag
     4381 gttaatgtac tgagtaaaaa tcgtataaag aatggggcat acatatttat ttatccccct
     4441 tagtcgtcct tttatttaag atttctctca gttcctcaat atcatcttca gatagtttat
     4501 cattttctaa aaagtttagt accatagaat taagagtgcc attataaaaa cgatttaaaa
     4561 aagaactact ttctgtagct acgtattctt cttctttaac aagtggagta taaacgaata
     4621 ctctgctatt tttttcatat gttaaagctc cttttttaac taatctcaat aacattgttt
     4681 gaattgtttt aggactccat gtacttgttt ctaccagctt ttcaattact tcagtagtgc
     4741 tgataggagc atatttccaa ataattttca taacttgata ttctgcttct gaaatatgtg
     4801 gtaacttttt catactatac ctccttctac ttacatttgt aatacataaa gtatattatc
     4861 attaatttag tttgtcaatt gttaaaaata attcatttat agttgattaa ataaacgtat
     4921 atagttaaat aaaattttta tttaagctgt gcaagtatag ctttaaacta aataaaaggt
     4981 ttatattgat agctaaaaat atagttgata aaaagccaaa gtggggtaat gagtgtgaaa
     5041 gatatatttt tagaattatg ttaatagtat atagttatat taatagcata tagttacact
     5101 aatataaaat aaaagtgcct tataaataga attttttgag gcacttatta tttgtctaaa
     5161 ttagttttta ttattttgca atctcaaccg tttataatct cgaacatttt ttttatatct
     5221 tctgatgcca tttcaaattt tgatttttca atcagtatgt ttccaccagt cataatatat
     5281 gatggaaata ctcttaaatt atcataataa agttcattat tacgaagttt atatgttgaa
     5341 atggaaggga tgacattctt ttttatatgt tttttagtaa tcatattaaa agatttcttt
     5401 gcaacatctc ccattaacat tactacctct aggtttttaa acaatttaat ttcttcttct
     5461 aaaataggca tatgtaattt aattgtatca cgagttattg tatattctga tttaggaagt
     5521 tttacggcat tagttatata gatacccata tctaggatat catttatatt agatacttct
     5581 attcctgcat tgttaaataa aggaatagta gtttttaaat aatcagcatc tttatctata
     5641 ctataaaaat cgtcattggt gttagttggg acaacttcat taatcattat agtttttatt
     5701 ttgtctgtgt ctattgttat attaggaagg taggatatta ttctgttgtc atcaaaaaga
     5761 ttattctgtt taataaattc atttaatgtt gattttatat ttattttcat ataacgagtc
     5821 tccttagttt taagctttaa attactataa aagtatagtt ttaaggtata taattttgaa
     5881 aatatagtaa catatttgaa agtataataa catataaaac atgacaacag aatgtcatgt
     5941 tttatcaata atctatcgca acttagaatt tattagagct tctgcctgtt cataatcgtc
     6001 tttatgaaca taaattaagt actcataaga gaaatcttct ttttgaccta aattgccaag
     6061 ggttatttca ttataaaaag gttttttaga gtaagtatta tttttcactt tatacttata
     6121 tttaatttta tttactgata atatattgca tatctcgtta aatctatcta aagaatatcc
     6181 tcggaaaagc tcagttctat ttaaaatcat aaggaaaacc tccttttaaa agtaatatat
     6241 tgaaaagcat aggagaaatg tactataagt tgttataaat aaaaggtagc aaatttaaat
     6301 tgatttgtaa agtttttatg agtattatat tagtgataaa acaaataatt tttagaatct
     6361 aactaataac aatttaaaat agataactta tgtaagaacc ataggaacta tgggggatat
     6421 cctgtggaga attagtaaga catattttag tatgcaaaat tctatgaatt aggaaccttg
     6481 tgactttagt catggaggtt cagaaggata tttaagtatt gaaaggagag tttaattgaa
     6541 aaaatatctc aagaaaaaat aagtacactt actcaagaag tatcacaaga aaaaagtgag
     6601 cagtggaaat atgtatatga aaaataatga tgaaataact gcttttaaag gattagataa
     6661 atatgatata aataattatt gtttagtagc agactatatt agatgtctaa agaaatataa
     6721 attaatagat tagtgtttga ttaataaatt agtgcttgat ttaaggatta tagagtaaat
     6781 ttgaagttag tgtgataatg aaactaagct taaatctaat ataactgtaa aacataaact
     6841 taaatagagt tgccaaaata aaattggtaa ctctattttt tattttaatt tttattaaaa
     6901 ttattaggtg ttatagaatt gttatttttt catgttagta ttctcttagt aatagattta
     6961 aagtattaca agaagggaga aataaatatg aaagatttaa ttattgaaac aaaaaactta
     7021 tcaaaaagat atggagaaca aatggcagtt aacaatgttt ctctacaaat tgaacgaaat
     7081 agtatatatg gacttttagg accaaatggt gcaggaaagt ccacaacact taaaatgctt
     7141 gttggtcttc ttcgcccaac agatggacgt attatttttg atagaaagcc ttggaaacga
     7201 gaatctcttg aaaaaatagg ttctttaata gaatctcctg cattgtatgg aaaccttaca
     7261 gcagaggaaa atttgttagt acatacaaaa atattgggaa tacctaaaga taaaatttat
     7321 gaggtgttgg aaatagtaaa tttgaaaaat acaggtaaaa agagagcttc acaattttct
     7381 atgggtatga aacaaaggtt aggaattgct attgcacttt tgaatgaccc agagttgtta
     7441 atactagatg agccaactaa tggtcttgac ccctttggca ttcaagaatt gcgtgaattg
     7501 attgcttctt ttccagaaaa aggtataact gtaattcttt ctagccatat tctatcagag
     7561 gttgctcaag ttgtagataa tataggaatt ataaatggag gaagactact atttcaagga
     7621 atgcctgacc ctaatgaaaa tctagaagaa ttttttacag atgttattct taaaggagga
     7681 caaaacagat gaatgcttta caatcagaac ttctaaagta taaaagaacc tttatgggta
     7741 aacttattgt ttttttccca gtatcttttg cagcatatgc atttataatg caatcaacac
     7801 ttatgcaaaa tccactatct caaataaatt cttgggcatg ggaaagtctt ttagcattga
     7861 tttttaactg gtggtcaatg atgtttctgt ctattggatt tgcattgttt gctactttgg
     7921 tagcttccca ggaaaaaaaa gctggaaatt atcgtgcttt acgtacacac aatgtgtctc
     7981 ccatgacaat ttggattaat aaaattatag ctatggcagt ttacagtttt atttcaacgc
     8041 tagttttaat agtcgtaaca attatgactg gtttaatatt gaaagctgga ccaattcctt
     8101 ttggacagat tataggggca agtattgtat gttgggttgg ttctcttgct atacttccaa
     8161 tccagctatg gctagctaca tggaaaggta tgttcctaag tatgggagtt ggagctttag
     8221 gcatgatatt tggtgtcctt gctgcgtcaa aaccattttg ggttgcagta ccttggagtt
     8281 gggcagttag aatggtatgc ccaatcatca acatacatcc taatggtact atattagaag
     8341 ctggagaccc acttttgaat acttcagtga ttcctattgg gattgttgtt tctttggttg
     8401 tgtttatagt attaacagca cttactgcag cttggttcaa taggagggat gacaaatgat
     8461 aaaactatta tcatcagaat ggcttagagc aaagaggacg gcagtccaag gtcttactct
     8521 ttatatgcct attatttttt cattatgtgt ggttgcttat ctcacaatta aatctggaag
     8581 tacacaagaa tttgcttttg aaggattttt tacaatatgg actgtattca tcattcctat
     8641 tggagtaggt attttagcag gatttatagt gcaggaggaa gagcttgctg gaaattttaa
     8701 tggtttttta tgtgtaggta tttctcgtgt tagattatat ctgggaaaat ttctttttct
     8761 tctgttttgc ctaactatat gcacatttat tgctacgttg atactctgca taggaatgaa
     8821 tattgcagtg ccatctggag caacaatatg gttattcctg tcagcagcag gacttgtggt
     8881 gattgggact cttccactat tagctattca tctttgggtg agttttgcat ggggtatggg
     8941 tgcgtctatt ggtattagta ttggtggaat tttgatggca gcaatacttg gattaactag
     9001 cgttggtgca aaagtctggg catttgttcc ctggacatgg ccagtaaaac taggtatgct
     9061 tccaggtact tattttatta aagaagctgg aactatttca actgaggtat tttactctaa
     9121 aatgatgcaa tcagctactg tagtatttat agtagtagca ataggtttga ttatattctt
     9181 gataggaggc gttatttggt ttaaaatatg ggagggaagg aaaagttacg aatagacgaa
     9241 tattgtttta atactaacct tcccataaga gaaaggtggt gttatattga gtaaaatatt
     9301 gattattgat gatgaaatag atttagtaat gctacttgag gacgaattga aggcaaaagg
     9361 gcatgaagtg ctaatagctt atgatggtca ggcaggtata gaactatcaa aaaatgagcc
     9421 tgacctcatt atactggata ttatgatgcc taaaatggat gggtttgagg tatgccaggc
     9481 aattagagat gatgttttgt gcccaattat attcttaagt gcaaaacaat cagaaaccga
     9541 caaaatcaaa gggttgacct ttggagggga tgattatgta acaaagccat ttggattgcg
     9601 tgaacttatg gcaaggattg aagctaatct gcgtagggaa aagcgttcac agtatatcaa
     9661 tgaggagaac aagaggtcta ctttgtattt tgaaaagtta tgcttatata tgaaggaacg
     9721 tgcaattaaa attgatggta aggatattga tttaacaaaa atagagtatg atattgttga
     9781 attactagca ctccatgcag gacaagtttt ctcaagagaa caaatctatg aaaaagtatg
     9841 ggggtgtgat tctgaaggtg attcatcaac tgttgttgaa cgagtcaaaa aaatacgagc
     9901 taagttttct gctgtgacac cagaaaaaga atacatttcc acagtttggg ggattgggta
     9961 taagtggaat aaaatgtaaa gggagagagg tcattaaatg aaaaatcaat cacttgccac
    10021 ccaattcagg cgtactttta tttttattat aatagctagt atagttgcca cagtgattac
    10081 ttatgtattt gctttttatt tgtttattta ttccttgaat aaggatattt atccaccaaa
    10141 ctattatgag cgacaagttc ctagaattaa aaaatacatt gatgaaaaaa acatagcttt
    10201 gttatcacaa tcaaatgaag aaggtttaaa aagaactatt cgcggggatg gtatgttata
    10261 tcaagttgtg gatgataatg gcaatattct atatggtaat aaccctaaaa aaccatttaa
    10321 aacaaaagaa gaattattta ataattttat aaatacaact gttcgtaaag gtggttatat
    10381 ttatactgtt ccaattaaag gtactaatgg aaaaattgag ggagctgtta ttctattcta
    10441 ccaagtgaaa atgacctttg caaatagtat ggggaggttg acgtttgcag ttattgtaat
    10501 agctatgttt tctccatttc tatatgttat aggttttact atatggcttt ctaagcagtt
    10561 tgtaaagaat atcaatcaac cattacatct attgattgat gcatccaaga agataaaaga
    10621 aaaagacctt gattttgaaa tcgactatta ttcagataat gaactcggta aactttgtag
    10681 tgccttttct gaaatgaaag acgaattaaa aaactcttta tctgcacagt ggaagatgga
    10741 gcaggaacgt gttgaaatgg tggaagctct agcacatgat ttaaaatctc ctctttctat
    10801 tgttttggga tatacggatg cacttattgg taataataca gatgacaatg aaaaacttca
    10861 taggtatctt acagtgataa gagaaaatac ggaaaaaagt gctgctcttg tccaaaagat
    10921 gcaatatacg tctgatttag aaaagtcaaa tatacaatta aatcctgttc ctataaattt
    10981 acctgaattt ttggtgcaaa aggtacaaga ctatgaatta caggctcatc aaaaggacgt
    11041 tgaacttata ttgaaaatgt atggtaatat acaatcacca attcaaatag acttagatag
    11101 attgacacga atttttgata atatcatatc taacagtctg caatacactc cgtcaggtgg
    11161 taatattagt attattgcaa aggctgaaaa aaattgtatt tcttatgaga tttgtgattc
    11221 tggcagtggg tttagctcta aagatttaaa aaaggctctt gataaatttt atcgaggaga
    11281 tgaagctaga cagactaaag gtgggcattc tggtcttggg ctttacattg tcaaacaatt
    11341 agtggaacag cttggagggt ctgttaaaat agaaaattca aaatcaggtg gagcatgtgt
    11401 gaaattttgg catagcattt aactatatat tttaaattaa aggggagtgt ctcagaacga
    11461 aatttttagt tcgtgaggca ctcttttttt gtgaagttaa atatattttc atcattccaa
    11521 caatgaaaaa aggtttatct ctattttgag acaggccttt ttttaaaact tatattatag
    11581 ttggaaaggt ttattagtta gatttgaaag aaaatggaga cagtcattga taatctgatt
    11641 tatattatta tctgtaaaat agttataaat tgatatctat acagtcaatt atattcattt
    11701 ttttaacttt tcttaatgga ataagagtag ctataataca tgcaagtata gaaataatag
    11761 tagcttgtaa aattgaagta attggtatat tagtaaaatc aagcttttct atagttgcca
    11821 tgtttataat aatagcacta gcatacccag caattgaacc aagtatagca gcaaaaattc
    11881 cataataaac accttcccat aaaaacatct tatataagct actattactc ataccaatag
    11941 ctcttttaac tccaatctca ttagtcctag tatttatatt tgtatgagtt gtgttaatga
    12001 tgtttaaagt accaatcaat cccacaaata atataactgc ccatgctaga agttctattt
    12061 gtttaaaact ctctttaagt tgcttatctg attcaatata tgatatccaa tgagagccag
    12121 gattattttc acaaatctgt tcaatagaca atcttatttg ttctaaatct gatttatctt
    12181 tagcataaat atttatctgg ttgaacttgt ttttatttgt caaggtgtta taaacagaat
    12241 catatacaat aatatcaaca ccatttaccc agccattacc ttgtaaaaag aacattttat
    12301 ctaccgtact atatatatct agttgttttt tgttaattgt gatattatct ttaggtttta
    12361 aatttgtata tttagttttt acacctggtg tagaaatagc aagtggattt tttataaaac
    12421 agatagaacc atttttaaag tcttctaatg tagaatctgt aatagaaggc attaagtctt
    12481 ttaaagtttg ttcatcaatt ccaatgattt ttaacatctc accactattt tcaaaattta
    12541 tatctgtatc tatttcgccc tgtttgtatt cagagtactt tataaaagaa gtgcgattga
    12601 cgtttttcat gttatctatt ttatctagta ttgacttatc aaaaccagat atttcatttg
    12661 ttaaggaaaa atctccttct tttaaattac ttactgacct agatacatct aatacattag
    12721 agaactcact cagtgcaacg aatactgtta tactcataaa taatgatagt atggttatta
    12781 tagttcttcc tttatttctt tttaagttta gccatgctag atgtctttca aagtttttta
    12841 ttgttttttc tcgtttaatt tttcttttga ttttggtagt actgccagcc atagcaattt
    12901 tgggagagac atgtgaagca tatattgctg atggtaatgc tgaaataaat gaaaaaatca
    12961 aagatatact agtgcttaat actaatggga acatatatgc tgttgtgttt ttatgtataa
    13021 gctcagtaat ttctttatta tcatttgcta gaagtatatc tggatttaag atattagtga
    13081 ccattccagt tatgccttta gaagatataa ttccaattac agctccaata ggtatggcaa
    13141 tagtacataa aattaagatt tgtaaaataa ctatcttata tatctggttt ggttcagctc
    13201 ctattcctct taaacatcca tactctttaa ttttctttgt aacagagatt tttaaaatat
    13261 tgtagattac aagtccagaa gccagtaaaa ccaataaggc aacaaataaa gataccaata
    13321 taatcataga tataccatca ctattagaac tattttcatc tttttcaaac tgtattttga
    13381 gagcatttaa gtaaatccaa ttatattgaa ttgaattgtg agaaagattt atttttttag
    13441 ctaattgatt tactatttct tgaaacttct cttgctgttt tattttaaaa tccaaagagg
    13501 ataaaatata tctcttaggt aatagatttt ctgatgcacc tttgcccaca ataccgttta
    13561 ctataccact tacgtatcct gtataatcat cctccaaaat accactcaat ttaaagttag
    13621 ctgtgtaatt atatggtggt attgtatcat ttaaaagtga aatatctaaa ttcataggta
    13681 tagtcactcc tagtcttggt tttataccta taagctttag tgtgttttca tctaaggcta
    13741 tttcgttttt atctttaggc aaatgtccag attttaattt cattaatttg gggtagttag
    13801 aaagtcctgt tttatcatac tcttttacaa gtacagagat accagaatcc tttattttac
    13861 tactgccaag agttattgaa gtaccagctt gataaactct atcatctttc ttta
//
LOCUS       DAFNNB010000101        13745 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0102, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000101 DAFNNB010000000
VERSION     DAFNNB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0102"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..6951
                     /gene="tcdB"
                     /locus_tag="L3M60_002468"
                     /gene_synonym="toxB"
     CDS             <1..6951
                     /gene="tcdB"
                     /locus_tag="L3M60_002468"
                     /gene_synonym="toxB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_074049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdB"
                     /protein_id="HBN6227123.1"
                     /translation="DINSLTDIYIDTYKKSGRNKALKKFKEYLVTEVLELKNNNLTPV
                     EKNLHFVWIGGQINDTAINYINQWKDVNSDYNVNVFYDSNAFLINTLKKTIVESAIND
                     TLESFRENLNNPRFDYNKFFRKRMEIIYDKQKNFINYYKAQREENPELIIDDIVKIYL
                     SNEYSKEIDELNTYIEESLNKIKQNSGNDVRNFEEFKNGESFKLYEQELVERWNLAAA
                     SDILRISALKEIGGMYLDVDMLPGIQPDLFESIEKPSSVTVDFWEMTKLEAIMKYKEY
                     IPGYTSEHFDMLDEEVQSSFESALASKSDKSEIFSSLGDMEASPLEVKIAFNSKGIIN
                     QGLISVKDSYCSNLIVKQIENRYKILNNSLNPAISEDNDFNTTTNTFIDSIMAEANAD
                     NGRFMMELGKYLRVGFFPDVKTTVNLSGPEAYAAAYQDLLMFKEGSMNIHLIEADLRN
                     FEISKTNISQSTEQEMASLWTFDDARAKVQFEEYKRNYFEGSLGEDDNLDFSQNIVVD
                     KEYLLEKISSLARSSERGYIHYIVQLQGDKISYEAACNLFAKTPYDSILFQKNIENSE
                     VAYYYNPGDGEIQEIDKYRIPSIISDRPKIKLTFIGHGKDEFNTDIFAGLDVDSLSTE
                     IETAIDLAKEDISSKSIEINLLGCNMFSYSINVEETYPGKLLLKVKDKISELMPSISQ
                     DSIIVSANQYEVRINNEGRRELLDHSGEWINKEESIIKDISSKEYISFNPKENKITVK
                     SKNLPELSTLLQEIRNNSNLSDIELEEKVMLAECEINVISNIDTQIVEERIEEAKNLT
                     SDSINYIKNEFKLIESISDSLYDLKQQNELDDSHFISFEDISKTEDGFSIRFINKETG
                     ESIFVETEKEIFSEYANHIEREISNIKDTIFDTVNGKLVKKVNLDAIHEVNTLNAAFF
                     IQSLIGYSSSKESLSNLSVAMKVQVYAQLFSTGLNTITDAAKVVELVSTALDETIDLL
                     PTLSEGLPVIATIIDGVSLGAAIKELSETSDPLLRQEIEAKIGIMAVNLTAATTAIIT
                     SSLGIASGFSILLVPLAGISAGIPSLVNNELVLRDKATKVVDYFKHISLVETEGAFTL
                     LDDKIMIPQDDLVISEIDFNNNSIVLGKCEIWRMEGGSGHTVTNDIDHFFSSPTITYI
                     KPHLSIYDVLEVQKEELDLSKDLMVLPNAPNRVFAWETGWTPGLRSLENEGTKLLDRI
                     RDHYKGEFYWRYFAFIADALITTLKPRYEDTNIRINLDSNNRSFIVPIITTEHIREKL
                     SYSFHGSGGTYALSLSQYNMGINIELSESDVWIIDVDNVVRDVTIDSDKIKKGDLIEG
                     ILSTLSIEDNKIILNHHEINFSGDVNGSNGFISLTFSILEGINAIIEVDLLSKSYKLL
                     ISGELKILMLNSNHIQQKIDYIGFNSELQKNIPYSFVDSEGKENGFINGSTKEGLFVS
                     ELPDVVLISKVYMDDSKPSFGYYSNNLKDVKVITKDNVNILTGYYLKDDIKISLSFTL
                     QDEKTIKLNGVHLDESGVAEILKFMNKKGSTNTSDSLMSFLESVNIKSIFVNFLQSKI
                     NFILDANFIISGTTSIGQFEFICDENDNIQPYFIKFNTLETTYTLYVGNRQNMIVEPN
                     YDLDDSGDISSTVINFSQKYLYGIDSCVNKVVISPNIYTDEINITPVYETNNNYPEVI
                     VLDANYINEKINVNINDLSIRYVWSNDGNDFILMSTSEENKVSQVKIRFVNVFKDKTL
                     ANKLSFNFSDKQDVPVSEIISAFTPSYYEDGLIGYDLGLVSLYNEKFYINNFGMMVSG
                     LIYINDSLYYFKPPVNNLITGFVTVGDDKYYFNPTNGGAASIGETIINDKNYYFNQSG
                     ILQTGVFSTEDGLKYFAPANTLDENLEGEAIDFTGKLIIDENIYYFEDNYRGAVEWKE
                     LDGEMYYFSPETGKAFKGLNQIGDDKYYFNSDGIMQKGFVSINDKKYYFDDSGVMKVG
                     YIEIDGKYFYFAENGEMQIGVFNTSDGFKYFAHHNEDLGNEEGEAISYSGILNFNNKI
                     YYFDYSFTAVVGWKDLEDGSKYYFDEDTAEAYVGLSLINDGQYYFNDDGIMQVGFVTI
                     NNKVFYFSDSGIIESGVQNIDDNYFYIDEKGIVQIGVFDTSDEYKYFAPANTVNDNIY
                     GQAVDYSGLVRVGEDIYYFGETYTIETGWIYDMENESDKYYFNPETKKACKGINLIDD
                     IKYYFDENGIMRTGLISFENNDYYFNENGEMQFGYINIEDKMFYFGEDGVMQIGVFNT
                     QDGFKYFAHQNTLDENFEGESINYTGWLDLDEKRYYFTDEYIAATGSVIIDGEEYYFD
                     PDTAQLVISE"
     gene            7073..7573
                     /gene="tcdE"
                     /locus_tag="L3M60_002469"
     CDS             7073..7573
                     /gene="tcdE"
                     /locus_tag="L3M60_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like glycosylating toxin export protein
                     TcdE"
                     /protein_id="HBN6227124.1"
                     /translation="MHSSSPFYISNGNKIFFYINLGGVMNMTISFLSEHIFIKLVILT
                     ISFDTLLGCLSAIKSRKFNSSFGIDGGIRKVAMIACIFFLSVVDILTKFNFLFMLPQD
                     CINFLRLKHLGISEFFSILFILYESVSILKNMCLCGLPVPKRLKEKIAVLLDAMTDEM
                     NAKDEK"
     gene            7652..7813
                     /locus_tag="L3M60_002470"
     CDS             7652..7813
                     /locus_tag="L3M60_002470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227125.1"
                     /translation="MKLIMKTREQMPRDTQVLNTYNFEASVHYYMDDKVVYQTLVHKD
                     GAWSVGKIY"
     gene            7902..8084
                     /locus_tag="L3M60_002471"
     CDS             7902..8084
                     /locus_tag="L3M60_002471"
                     /inference="COORDINATES: protein motif:HMM:NF022413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227126.1"
                     /translation="MRDEGQNDRENKYQTLINDLIDSLKVIGKIYKEIKTLKKYKICL
                     QITIRQSPYLIEESIN"
     gene            8301..>13745
                     /gene="tcdA"
                     /locus_tag="L3M60_002472"
                     /gene_synonym="toxA"
     CDS             8301..>13745
                     /gene="tcdA"
                     /locus_tag="L3M60_002472"
                     /gene_synonym="toxA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin TcdA"
                     /protein_id="HBN6227127.1"
                     /translation="MSLISKEELIKLAYSIRPRENEYKTILTNLDEYNKLTTNNNENK
                     YLQLKKLNESIDVFMNKYKNSSRNRALSNLKKDILKEVILIKNSNTSPVEKNLHFVWI
                     GGEVSDIALEYIKQWADINAEYNIKLWYDSEAFLVNTLKKAIVESSTTEALQLLEEEI
                     QNPQFDNMKFYKKRMEFIYDRQKRFVNYYKSQINKPTVPTIDDIIKSHLVSEYNRDET
                     LLESYRTNSLRKINSNHGIDIRANSLFTEQELLNIYSQELLNRGNLAAASDIVRLLAL
                     KNFGGVYLDVDMLPGIHSDLFKTISRPSSIGLDRWEMIKLEAIMKYKKYINNYTSENF
                     DKLDQQLKDNFKLIIESKSEKSEIFSKLENLNVSDLEIKIAFALGSVINQALISKQGS
                     YLTNLVIEQVKNRYQFLNQHLNPAIESDNNFTDTTKIFHDSLFNSATAENSMFLTKIA
                     PYLQVGFMPEARSTISLSGPGAYASAYYDFINLQENTIEKTLKASDLIEFKFPENNLS
                     QLTEQEINSLWSFDQASAKYQFEKYVRDYTGGSLSEDNEVDFNKNTALDKNYLLNNKI
                     PSNNVEEAGSKNYVHYIIQLQGDDISYEATCNLFSKNPKNSIIIQRNMNESAKSYFLS
                     DDGESILELNKYRIPERLKNKEKVKVTFIGHGKDEFNTSEFARLSVDSLSNEISSFLD
                     TIKLDISPKNVEVNLLGCNMFSYDFNVEETYPGKLLLSIMDKITSTLPDVNKDSITIG
                     ANQYEVRINSEGRKELLAHSGKWINKEEAIMSDLSSKEYIFFDSIDNKLKAKSKNIPG
                     LASISEDIKTLLLDTSVSPDTKFILNNLKLNIESSVGDYIYYEKLEPVKNIIHNSIDD
                     LIDEFNLLENVSDELYELKKLNNLDEKYLISFEDISKNNSTYSVRFINKSNGESVYVE
                     TEKEIFSKYSEHITKEISTIKNSIITDVNGNLLDNIQLDHTSQVNTLNAAFFIQSLID
                     YSSNKDLLNDLSTSVKVQLYAQLFSTGLNTIYDSIQLVNLISNAVNDTINVLPTITEG
                     IPIVSTILDGINLGAAIKELLDEHDPLLKKELEAKVGVLAINMSLSIAATVASIVGIG
                     AEVTIFLLPIAGISAGIPSLVNNELILHDKATSVVNYFNHLSESKEYGPLKTEDDKIL
                     VPIDDLVISEIDFNNNAIKLGTCNILAMEGGSGHTVTGNIDHFFSSPSISSHIPSLSI
                     YSAIGIKTENLDFSKKIMMLPNAPSRVFWWETGAVPGLRSLENNGTKLLDSIRDLYPG
                     KFYWRFYAFFDYAITTLKPVYEDTNIKIKLDKDTRNFIMPTITTNEIRNKLSYSFDGA
                     GGTYSLLLSSYPISTNINLSKDDLWIFNIDNEVREISIENGTIKKGKLIKDVLSKIDI
                     NKNKLIIGNQTIDFSGDIDNKDRYIFLTCELDDKISLIIEINLVAKSYSLLLSGDKNY
                     LISNLSNTIEKINTLGLDSKNIAYNYTDESNNKYFGAISKTSQKSIIHYKKDSKNILE
                     FYNGSTLEFNSKDFIAEDINVFMKDDINTITGKYYVDNNTDKSIDFSISLVSKNQAKV
                     NGLYLNESVYSSYLDFVKNADGHHNTSNFMNLFLDNISFWKLFGFENINFVVDKYFTL
                     VGKTNLGYVEFICDNNKNIDIYFGEWKTSSSKSTIFSGNGRNVVVEPIYNPDTGEDIS
                     TSLDFSYEPLYGIDRYINKVLIAPDLYTSLININTNYYSNEYYPEIIVLNPNTFHKKV
                     NINLDSSSFEYKWSTEGSDFILVRYLEESNKKILQKIRIKGILSNTQSFNKMSIDFKD
                     IKKLSLVYIMSNFKSFNSENELDRDHLGFKI"
BASE COUNT         5560 a         1462 c         2163 g         4560 t
ORIGIN      
        1 gatataaata gtttaacaga tatttatata gatacatata aaaaatctgg tagaaataaa
       61 gccttaaaaa aatttaagga atatctagtt acagaagtat tagagctaaa gaataataat
      121 ttaactccag ttgagaaaaa tttacatttt gtttggattg gaggtcaaat aaatgacact
      181 gctattaatt atataaatca atggaaagat gtaaatagtg attataatgt taatgttttt
      241 tatgatagta atgcattttt gataaacaca ttgaaaaaaa ctatagtaga atcagcaata
      301 aatgatacac ttgaatcatt tagagaaaac ttaaataacc ctagatttga ctataataaa
      361 tttttcagaa aacgtatgga aataatctat gataaacaga aaaatttcat aaactactat
      421 aaagctcaaa gagaagaaaa tcctgaactt ataattgatg atattgtaaa gatatatctt
      481 tcaaatgagt attcaaaaga gatagatgaa cttaatacct atattgaaga atccttaaat
      541 aaaattaaac agaatagtgg aaatgatgtt agaaactttg aagaatttaa aaatggagag
      601 tcattcaaat tatatgaaca agagttggta gaaagatgga atttagctgc tgcttctgac
      661 atattaagaa tatctgcatt aaaagaaatt ggtggtatgt atttagatgt tgatatgtta
      721 ccaggaatac aaccagactt atttgagtct atagagaaac ctagttcagt aacagtggat
      781 ttttgggaaa tgacaaagtt agaagctata atgaaataca aagaatatat accaggatat
      841 acttcagaac attttgacat gttagatgaa gaagttcaaa gtagttttga atctgctcta
      901 gcttctaagt cagataaatc agaaatattc tcatcacttg gtgatatgga ggcatcacca
      961 ctagaagtta aaattgcatt taatagtaag ggtattataa atcaagggct aatttctgtg
     1021 aaagactcat attgtagcaa tttaatagta aaacaaatcg agaacaggta taaaatattg
     1081 aataatagtt taaatccagc tattagcgag gataatgatt ttaatactac aacgaatacc
     1141 tttattgata gtataatggc tgaagctaat gcagataatg gtagatttat gatggaacta
     1201 ggaaagtatt taagagttgg tttcttccca gatgttaaaa ctactgttaa cttaagtggc
     1261 cctgaagcat atgcggcagc ttatcaagat ttattaatgt ttaaagaagg cagtatgaat
     1321 atccatttga tagaagctga tttaagaaac tttgaaatct ctaaaactaa tatttctcaa
     1381 tcaactgaac aagaaatggc tagcttatgg acatttgacg atgcaagagc taaagttcaa
     1441 tttgaagaat ataaaaggaa ttattttgaa ggttctcttg gtgaagatga taatcttgat
     1501 ttttctcaaa atatagtagt tgacaaggag tatcttttag aaaaaatatc ttcattagca
     1561 agaagttcag agagagggta tatacactat attgttcagt tacaaggaga taaaattagt
     1621 tatgaagcag catgtaactt atttgcaaag actccttatg atagtatact gtttcagaaa
     1681 aatatagaaa attcagaagt tgcatattat tataatcctg gagatggtga aatacaagaa
     1741 atagacaagt atagaattcc aagtataatt tctgatagac ctaagattaa attaacattt
     1801 attggtcatg gtaaagatga atttaatact gatatatttg caggtcttga tgtagattca
     1861 ttatctacag aaatagaaac agcaatagat ttagccaaag aggatatttc ttctaagtca
     1921 atagaaataa atttattagg atgtaatatg tttagctact ctatcaacgt agaagagact
     1981 tatcctggaa agttattact taaagttaaa gataaaatat cagaattaat gccatctata
     2041 agtcaagact ctattatagt aagtgcaaat caatatgaag ttagaataaa taatgaagga
     2101 agaagagaat tattggatca ttctggtgaa tggataaata aagaagaaag tattataaag
     2161 gatatttcat caaaagaata tatatcattt aatcctaaag aaaataaaat tacagtaaaa
     2221 tctaaaaatt tacctgagct gtctacatta ttacaagaaa ttagaaataa ttctaattta
     2281 agtgatattg aactagaaga aaaagtaatg ttagcagaat gtgaaataaa tgttatttca
     2341 aatatagata cacaaattgt tgaggaaagg attgaagaag ctaagaattt aacttctgac
     2401 tctattaatt atataaaaaa tgaatttaaa ctaatagaat ctatttctga ttccttatac
     2461 gatttaaaac aacaaaatga attggatgat tctcatttta tatcttttga ggatatatca
     2521 aagacagaag atggatttag tataagattt attaataaag aaactggaga atctatattt
     2581 gtagaaactg aaaaagaaat tttttctgaa tatgctaatc atatagagag agaaatttct
     2641 aatataaaag atactatatt tgatactgta aatggtaagt tagtaaaaaa agtgaattta
     2701 gatgctatac atgaagtaaa tactttaaat gctgcatttt ttatacaatc attaatagga
     2761 tatagtagtt ctaaagaatc tcttagtaat ttaagtgtag caatgaaagt tcaagtttat
     2821 gctcaattat ttagcactgg tttaaatact attacagatg cagccaaagt tgttgaatta
     2881 gtatcaactg cattagatga aactatagac ttacttccta cattatctga aggattacct
     2941 gtaattgcaa ctatcataga tggtgtaagt ttaggtgcag caatcaaaga gttaagtgaa
     3001 actagtgacc cattattaag acaagaaata gaagctaaga taggtataat ggcagtaaac
     3061 ttaacagcag ctacaactgc aatcattact tcatctttag ggatagctag tggatttagt
     3121 atacttttag ttcctttagc aggtatttca gcaggtatac caagcttagt aaataatgaa
     3181 cttgtactta gagataaggc aacaaaggtt gtagattatt ttaaacatat ttcattagtt
     3241 gaaactgaag gagcatttac tttattagat gataaaataa tgataccaca agacgattta
     3301 gtgatatcag aaatagattt taataataat tcaatagttt taggtaaatg tgaaatctgg
     3361 cgaatggaag gtggctcagg tcatacagta acaaatgata tagatcactt cttttcatca
     3421 ccaacaataa catatataaa accacactta tctatctatg atgtattgga agtacaaaaa
     3481 gaagaacttg atttgtcaaa agatttaatg gtattaccta atgctccaaa tagagtattt
     3541 gcttgggaaa caggatggac accaggttta agaagtttag aaaatgaggg tacaaaactg
     3601 ttagaccgta taagagatca ctacaaaggt gagttttatt ggagatattt cgcttttata
     3661 gctgatgctt taataacaac attaaaacca cgatatgaag atactaatat aaggataaat
     3721 ttagatagta acaatagaag ttttatagtt ccaataataa ctacagaaca tataagagaa
     3781 aaattatcat attctttcca tggctcagga ggaacatatg cattatctct ttctcaatat
     3841 aatatgggta taaatataga attaagtgaa agtgatgttt ggattataga tgttgataat
     3901 gttgtgagag atgtaactat agactctgat aaaattaaaa aaggtgattt aatagaaggt
     3961 attctatcta cactaagtat tgaagataat aaaatcatct taaatcatca tgagattaat
     4021 ttttctggag atgtaaatgg aagtaatgga tttatttctt taacattttc aattttagaa
     4081 ggaataaatg caattataga agttgattta ttatctaaat catataaatt acttatttct
     4141 ggcgaattaa aaatattgat gttaaattca aatcatattc aacagaaaat agattatata
     4201 ggattcaata gcgaattaca gaaaaatata ccatatagct ttgtagatag tgaaggaaaa
     4261 gagaatggtt ttattaatgg ttcaacaaaa gaaggtttat ttgtatctga attacctgat
     4321 gtagttctta taagtaaggt ttatatggat gatagtaagc cttcatttgg atattatagt
     4381 aataatttga aagatgtcaa agttataact aaagataatg ttaatatatt aacaggttat
     4441 tatcttaagg atgatataaa aatctctctt tcttttactc tacaagatga aaaaactata
     4501 aaattaaatg gtgtgcattt agatgaaagt ggagtagctg agattttgaa gttcatgaat
     4561 aaaaaaggta gtacaaatac ttcagattct ttaatgagtt ttttagaaag tgtgaatata
     4621 aaaagtattt tcgttaattt cttacaatct aaaattaact ttatattaga tgctaatttt
     4681 ataataagtg gtactacttc tattggtcaa tttgagttta tttgtgatga aaatgataat
     4741 atacaaccat atttcattaa gtttaataca ctagaaacta cttatacttt atatgtagga
     4801 aatagacaaa atatgatagt agaaccaaat tatgatttag atgattctgg agatatatct
     4861 tcaactgtta tcaatttctc tcaaaagtat ctttatggaa tagacagttg tgttaataaa
     4921 gttgtaattt caccaaatat ttatacagat gaaataaata taacgcctgt atatgaaaca
     4981 aataataatt atccagaagt tattgtatta gatgcaaatt atataaacga aaaaataaat
     5041 gttaatatca atgatctatc tatacgatat gtatggagta atgatggtaa tgattttatt
     5101 cttatgtcaa ctagtgaaga aaataaggtg tcacaagtta aaataagatt cgttaatgtt
     5161 tttaaagata agactttggc aaataagcta tctttcaact ttagtgataa gcaagatgtg
     5221 cctgtgagtg aaataatctc agcatttaca ccttcatatt atgaggatgg attgattggc
     5281 tatgatttgg gtctagtttc tttatataat gaaaaatttt atattaataa ctttggaatg
     5341 atggtatctg gattaatata tattaatgat tcattatatt actttaaacc accagtaaat
     5401 aatttgataa ctggatttgt tactgtaggt gatgataagt attactttaa tccaactaat
     5461 ggtggagctg cctcaattgg agagacaata attaatgaca aaaattatta tttcaaccaa
     5521 agtggaatct tacaaacagg tgtatttagt acagaagatg gacttaaata ttttgcccca
     5581 gctaatacac ttgatgaaaa cctagaagga gaagcaattg attttactgg aaaattaatt
     5641 attgacgaaa atatttatta ttttgaagat aattatagag gagctgtaga atggaaagaa
     5701 ttagatggtg aaatgtacta ttttagccca gaaacaggca aagcttttaa aggtctaaat
     5761 caaataggtg atgataaata ctattttaat tctgatggaa ttatgcaaaa aggatttgtt
     5821 agtataaatg ataagaaata ttattttgat gattctggtg ttatgaaagt gggttatatt
     5881 gaaatagatg gcaagtattt ctactttgct gaaaatggag aaatgcaaat aggagtattt
     5941 aatacatcag atggatttaa atattttgct catcataatg aagacctagg aaatgaagaa
     6001 ggtgaagcaa tttcatattc tggtatatta aatttcaata ataaaattta ctattttgat
     6061 tattcattta cagctgtagt tggatggaaa gatttagagg atggttcaaa gtattatttt
     6121 gatgaagata cagcagaagc atatgtaggt ttatcattaa tcaatgatgg tcaatattat
     6181 tttaatgatg atggaattat gcaagttgga tttgtcacta taaataataa agttttctac
     6241 ttctctgatt ctggaattat agaatctgga gtacaaaata tagatgataa ttatttctat
     6301 atagatgaga agggtatagt tcaaattggc gtatttgata cttcagatga atataaatac
     6361 tttgcacctg ctaatactgt aaatgataat atttacggac aagcagttga ctatagcggt
     6421 ttagttagag ttggtgaaga tatatattat tttggagaaa cctatacaat tgagactgga
     6481 tggatatatg atatggaaaa tgaaagtgat aaatattatt tcaatccaga aactaaaaaa
     6541 gcatgcaaag gtattaattt aattgatgat ataaaatatt attttgatga gaatggcata
     6601 atgagaacgg gtcttatatc atttgaaaat aatgattatt actttaacga gaatggtgaa
     6661 atgcaatttg gttatataaa tatagaagat aagatgtttt attttggtga agatggtgtc
     6721 atgcagattg gagtatttaa tacgcaagat ggatttaaat actttgcaca tcaaaatact
     6781 ttggatgaga attttgaggg agaatcaata aactatactg gttggttaga tttagatgaa
     6841 aagagatatt attttacaga tgaatatatt gcagcaactg gttcagttat tattgatggt
     6901 gaggagtatt attttgatcc tgatacagct caattagtga ttagtgaata gataaaaata
     6961 tgttaaatat atcctcttat acttaaatat ataaaaataa acaaaatgat acactacata
     7021 aagtgttcta tctaatatga agatttacca ataaaaaggt gaactatgat gaattcacag
     7081 tagttcacct ttttatattt ctaatggtaa caaaatattt ttttatataa acctaggagg
     7141 cgttatgaat atgacaatat cttttttatc agagcatata tttataaagt tagtaatttt
     7201 aactatatca tttgatacat tattaggatg tttaagtgca ataaaaagtc gtaaatttaa
     7261 ttctagtttt ggaatagatg gaggaatcag aaaagtagca atgatagcat gtatattttt
     7321 tttatcagta gttgacattc ttacaaagtt taacttttta tttatgttac cacaagattg
     7381 tatcaatttt ttaagactaa aacatcttgg aatatctgaa tttttctcta ttttatttat
     7441 tttatatgaa agtgtaagta tattaaaaaa tatgtgctta tgtggattac cagtacctaa
     7501 gagattaaag gaaaaaatag cagttttact agatgcaatg acagatgaaa tgaatgctaa
     7561 ggatgaaaag taagtactgg tagatataat aaagatatta acaaataaaa agtgttatcc
     7621 aaataagaat agctgaaagt tatcataatt catgaaactg ataatgaaaa cgagggagca
     7681 gatgccaaga gacacacaag tattaaatac atataatttc gaagcaagtg ttcattacta
     7741 tatggatgac aaggtagtat atcaaacatt ggttcacaaa gatggtgcat ggtcagttgg
     7801 taaaatctat taagctacat tagttacaga tatcacaaac tataatagtt aaacatagaa
     7861 atatgtgtaa attgtgatgg aaattattca aaaacacaaa aatacgtgat gaaggacaaa
     7921 atgatagaga aaataagtat caaaccttaa taaatgattt aattgatagt ttaaaagtta
     7981 taggaaaaat atataaagaa ataaaaacat taaaaaaata taagatatgt ttacaaatta
     8041 ctatcagaca atctccttat ctaatagaag agtcaattaa ctaattgagt atctttaaat
     8101 tgaaatgtta ggaagtgatt taaatatgaa aacttaaatt ataaaaaatc aatattaatt
     8161 tatttttaaa aaatagaaag gagtgtataa gatttatttt caaagtttaa aaaaaagaaa
     8221 atcaatttaa atttcagaag gaataaatgt ggttatagaa gtggatttat tatcaaaaat
     8281 aataatacta ggaggttttt atgtctttaa tatctaaaga agagttaata aaactcgcat
     8341 atagcattag accaagagaa aatgagtata aaactatact aactaattta gacgaatata
     8401 ataagttaac tacaaacaat aatgaaaata aatatttaca attaaaaaaa ctaaatgaat
     8461 caattgatgt ttttatgaat aaatataaaa attcaagcag aaatagagca ctctctaatc
     8521 taaaaaaaga tatattaaaa gaagtaattc ttattaaaaa ttccaataca agccctgtag
     8581 aaaaaaattt acattttgta tggataggtg gagaagtcag tgatattgct cttgaataca
     8641 taaaacaatg ggctgatatt aatgcagaat ataatattaa actgtggtat gatagtgaag
     8701 cattcttagt caatacacta aaaaaggcta tagttgaatc ttctaccact gaagcattac
     8761 agctactaga ggaagagatt caaaatcctc aatttgataa tatgaaattt tacaaaaaaa
     8821 ggatggaatt tatatatgat agacaaaaaa ggtttgtaaa ttattataaa tctcaaatca
     8881 ataaacctac agtacctaca atagatgata ttataaagtc tcatctagta tctgaatata
     8941 atagagatga aactttatta gaatcatata gaacaaattc tttgagaaaa ataaatagta
     9001 atcatgggat agatatcaga gctaatagtt tgtttacaga acaagagtta ctaaatattt
     9061 atagtcagga gttgttaaat cgtggaaatt tagctgcagc atctgacata gtaagattat
     9121 tagccctaaa aaattttggc ggagtatatt tagatgttga tatgcttcca ggtattcact
     9181 ctgatttatt taaaacaata tctagaccta gctctattgg actagaccgt tgggaaatga
     9241 taaaattaga ggctattatg aagtataaaa aatatataaa taattataca tcagaaaact
     9301 ttgataaact tgatcaacaa ttaaaagata attttaaact cattatagaa agtaaaagtg
     9361 aaaaatctga gatattttct aaattagaaa atttaaatgt atctgatctt gaaattaaaa
     9421 tagctttcgc tttaggcagt gttataaatc aagccttgat atcaaaacaa ggttcatatc
     9481 ttactaacct agtaatagaa caagtaaaaa atagatatca atttttaaac caacacctta
     9541 acccagccat agagtctgat aataacttca cagatactac taaaattttt cacgattcat
     9601 tatttaattc agctaccgca gaaaactcta tgtttttaac aaaaatagca ccatacttac
     9661 aagtaggttt tatgccagaa gctcgctcca caataagttt aagtggtcca ggagcttatg
     9721 catcagctta ctatgatttc ataaatttac aagaaaatac tatagaaaaa actttaaaag
     9781 catcagattt aatagaattt aaattcccag aaaataatct atctcaattg acagaacaag
     9841 aaataaatag tctatggagc tttgatcaag caagtgcaaa atatcaattt gagaaatatg
     9901 taagagatta tactggtgga tctctttctg aagacaatga ggtagacttt aataaaaata
     9961 ctgccctcga taaaaactat ttattaaata ataaaattcc atcaaacaat gtagaagaag
    10021 ctggaagtaa aaattatgtt cattatataa tacagttaca aggagatgat ataagttatg
    10081 aagcaacatg caacctattt tctaaaaatc ctaaaaatag cattattata caacgaaata
    10141 tgaatgaaag tgcaaaaagt tactttttaa gtgatgatgg agaatctatt ttagaattaa
    10201 ataaatatag gatacctgaa agattaaaaa ataaggaaaa agtaaaagtg acctttattg
    10261 gacatggtaa agatgaattc aacacaagcg aatttgctag attaagtgta gattcacttt
    10321 ccaatgagat aagttcattt ttagatacca taaaattaga tatatcacct aaaaatgtag
    10381 aagtaaactt gcttggatgt aatatgttta gttatgattt taatgttgaa gaaacttatc
    10441 ctggtaagtt actattaagt attatggaca aaattacttc cactttacct gatgtaaata
    10501 aagattctat tactatagga gcaaatcaat atgaagtaag aattaatagt gagggaagaa
    10561 aagagcttct agctcactca ggtaaatgga taaataaaga ggaagctatt atgagcgatt
    10621 tatctagtaa agaatacatt ttttttgact ctatagataa taagctaaaa gcaaagtcca
    10681 agaatattcc aggattagcg tcaatatcag aagatataaa aacattgtta cttgatacaa
    10741 gtgttagtcc tgatacaaaa tttattttaa ataatcttaa gcttaatatt gaatcttctg
    10801 ttggtgatta catttattat gaaaaattag aacctgttaa aaatataatc cacaattcta
    10861 tagatgattt aatagatgag ttcaatctac ttgaaaatgt atctgatgaa ttatatgaat
    10921 taaaaaaatt aaataatcta gatgagaagt atttaatatc ttttgaagat atctcaaaaa
    10981 ataattcaac ttattctgta agatttatta acaaaagtaa tggtgaatca gtttatgtag
    11041 agacagaaaa agaaattttt tcaaaatata gcgaacatat tacaaaagaa ataagtacta
    11101 taaaaaatag tataattaca gatgttaatg gtaatttatt agataatata cagttagatc
    11161 atacatctca agttaataca ttaaacgcag cattctttat tcaatcatta atagattata
    11221 gtagcaataa agatctactg aatgatttaa gtacctcagt taaggttcaa ctttatgctc
    11281 aactatttag tacaggttta aatactatat atgactctat ccaattagta aacttaatat
    11341 caaatgcagt aaatgatact ataaatgtac tacctacaat aacagagggg atacctattg
    11401 tatctactat attagacgga ataaacttag gtgcagcaat taaggaatta ctagacgaac
    11461 atgacccatt actaaaaaaa gaactagaag ctaaggtggg tgttttagca ataaatatgt
    11521 cattatctat agctgcaacg gtagcttcaa ttgttggaat aggtgctgaa gttactattt
    11581 tcttattacc tatagctggt atatctgcag gaataccttc attagttaat aatgaattaa
    11641 tattgcatga taaggcaact tcagtggtaa attattttaa tcatttgtct gaatctaaag
    11701 aatatggccc tcttaaaaca gaagatgata aaattttagt tcctattgat gatttagtaa
    11761 tatcagaaat agattttaat aataatgcga taaaactagg aacatgtaat atattagcaa
    11821 tggagggggg atcaggacac acagtgactg gtaatataga tcactttttc tcatctccat
    11881 ctataagttc tcatattcct tcattatcaa tttattctgc aataggtata aaaacagaaa
    11941 atctagattt ttcaaaaaaa ataatgatgt tacctaatgc tccttcaaga gtattttggt
    12001 gggaaactgg agcagttcca ggtttaaggt cattagaaaa taatggaact aaattacttg
    12061 attcaataag agatttatac ccaggcaaat tttactggag attctatgcc tttttcgatt
    12121 atgcaataac tacattaaaa ccagtgtatg aagacactaa tattaaaatt aaactagata
    12181 aagatactag aaacttcata atgccaacta taactactaa cgaaattaga aacaaattat
    12241 cttattcatt tgatggagca ggaggaactt actctttatt attatcttcg tatccaatat
    12301 caacaaatat aaatttatct aaagatgatt tatggatatt taatattgat aatgaagtaa
    12361 gagaaatatc tatagaaaat ggtactatta aaaaaggaaa gttaataaaa gatgttttaa
    12421 gtaaaattga tataaataaa aataaactta ttataggcaa tcaaacaata gatttttcag
    12481 gcgatataga taataaagac agatatatat tcttgacttg tgagttagat gataaaatta
    12541 gtttaataat agaaataaat cttgttgcaa agtcttatag tttgttattg tctggagata
    12601 aaaattattt gatatccaat ttatctaata ctattgagaa aatcaatact ttaggtctag
    12661 atagtaaaaa tatagcgtac aattacactg atgaatctaa taataaatat tttggagcta
    12721 tatctaaaac aagtcaaaaa agcataatac attataaaaa agacagtaaa aatatattag
    12781 aattttataa tggcagtaca ttagaattta acagtaaaga ttttattgct gaagatataa
    12841 atgtatttat gaaagatgat attaatacta taacaggaaa atactatgtt gataataata
    12901 ctgataaaag tatagatttc tctatttctt tagttagtaa aaatcaagca aaagtaaatg
    12961 gattatattt aaatgaatcc gtatactcat cttatcttga ttttgtgaaa aatgcagatg
    13021 gacaccataa tacttctaat tttatgaatt tatttttgga caatataagt ttctggaaat
    13081 tgtttgggtt tgaaaatata aattttgtag tcgataaata ctttaccctt gttggtaaaa
    13141 ctaatcttgg atatgtagaa tttatttgtg acaataataa aaatatagat atatattttg
    13201 gtgaatggaa aacatcgtca tctaaaagca ctatatttag cggaaatggt agaaatgttg
    13261 tagtagagcc tatatataat cctgatacgg gtgaagatat atctacttca ctagattttt
    13321 cttatgaacc tctctatgga atagatagat atatcaataa agtattgata gcacctgatt
    13381 tatatacaag tttaataaat attaatacca attattattc aaatgagtac taccctgaga
    13441 ttatagttct taacccaaat acattccaca aaaaagtaaa tataaattta gatagttctt
    13501 cttttgagta taaatggtct acagaaggaa gtgactttat tttagttaga tacttagaag
    13561 aaagtaataa aaaaatatta caaaaaataa gaatcaaagg tatcttatct aatactcaat
    13621 catttaataa aatgagtata gattttaaag atattaaaaa actatcatta gtatatataa
    13681 tgagtaattt taaatcattt aattctgaaa atgaattaga tagagatcat ttaggattta
    13741 aaata
//
LOCUS       DAFNNB010000102        13589 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0103, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000102 DAFNNB010000000
VERSION     DAFNNB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0103"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..706)
                     /locus_tag="L3M60_002473"
     CDS             complement(<1..706)
                     /locus_tag="L3M60_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2140 domain-containing protein"
                     /protein_id="HBN6227128.1"
                     /translation="MGKVAKILLSLIVIVLVLAGIVVYILTPKERYDVSSQNNKIIDE
                     EYLSKGSYINSMEVVKNPLRMVGKISVSEEEFKNLIYTLMQKHGINELENNFVEMKNG
                     KIKVAGPYKVFGLINSQYELELRPTLQEGNLVVSLENVKLGKFKLSDKMLEKILSNYN
                     KKVPFEVNGNKITLEKSYLYPITLKNISIKKGNVDLDMEVEIDLKSQINGALGYLKDS
                     GKVQDILNHLINLKKTS"
     gene            998..2662
                     /locus_tag="L3M60_002474"
     CDS             998..2662
                     /locus_tag="L3M60_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HBN6227129.1"
                     /translation="MSNETNSSNFIKNIIINDLETGKHDSIITRFPPEPNGYLHIGHA
                     KSICLNFGLAKEFNGKANLRFDDTNPLKEDVEYVESIKEDVKWLGFDWNELNFASNYF
                     DEMYKRALILIKKGKAYVCDLTQEEMREYRGTLTEPGKESPHRNRTIEENLDLFERMK
                     NGEFKDGEKTLRAKIDMSSPNINLRDPIIYRISHSTHHNTGDKWCIYPMYAFAHPIED
                     AIEGITHSICTLEFEDQRPLYDWFVKECEMENIPRQIEFARLNINNTVMSKRKLKQLV
                     DEGIVDGWDDPRVPTISGIRRKGYTAEALRNFCSEIGVSKVNSTVDSQMLDYFLRENL
                     QPKAPLTMGVLRPLKLIITNYPENKTEMLEIENNAKDESQGKRLVPFSRELYIEQDDF
                     MEEPVKKYFRFFPGNEVRLKGAYFVKCTDVIKDENGNVVEIHGTYDPETKSGSGFTGR
                     KVKSTIHWVDAKSAIPCEFRLFEPLILDDLPENEGKHFLEQINPNSLEILQGFVEPTQ
                     IKDAKPFDKFQFVRNGFFSIDNKYTTDEKFVFNRIVPLKSSFKPAK"
     gene            complement(2769..3668)
                     /locus_tag="L3M60_002475"
     CDS             complement(2769..3668)
                     /locus_tag="L3M60_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HBN6227130.1"
                     /translation="MKKVKLIYNPNSGEKKILSNLDTIIELYQEQNYLLIPYRLTIKE
                     PVKNAFKEVDSSYEHILIAGGDGTVDLVVNAMKELNIKIPIGILPTGTANDFSNALQI
                     SFDIKEAINEIINSKPKKIDIGKVNNKYFINVASAGMFTDVSQRINTDLKNSFGRISY
                     YIKGIEEALYMRKFKIKVSSEEMYYDGDMYLMLIFNGKTAGNINLAYKAEIDDGYLDV
                     IIFKGMPIPKSIPVLISVLRGDYLDQYNGNEILYFKTKKVYIECADSLITDIDGEKGP
                     DFPLNIECIKDGIEVMGICSEKS"
     gene            complement(3695..4333)
                     /locus_tag="L3M60_002476"
     CDS             complement(3695..4333)
                     /locus_tag="L3M60_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBN6227131.1"
                     /translation="MDQNLNWLNEKRIEKTIKSLENNNMKGYLVSNKEELLKKIEEIV
                     KVGSLVGCGGSMTLFETGVIDFIREKYNFLDRYAKDIKPEEMKEIYRKSFSADAYFTS
                     TNAITEDGELYNLDGNGNRVAAMIYGPDKVIVIAGVNKIVKDVDEAIKRTKTIAAPAN
                     CKRLSTNTGCKSTGICIDCSSPGRICCSYTLIKRQLTPERIHVIFLNEDFGY"
     gene            complement(4673..5671)
                     /locus_tag="L3M60_002477"
     CDS             complement(4673..5671)
                     /locus_tag="L3M60_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4003 domain-containing protein"
                     /protein_id="HBN6227132.1"
                     /translation="MEQSFINGKVNLLIENYKALNEVKGSWQMGLIQHSCALAFTLKN
                     KRISPRLVEERIELIKKNTGLFSNFRGYNMFYMATLLSFESNPESSFKMILDIYKELK
                     SEKFWGDTYLPLTASIVYENREKMDYLTCISKMKIIYNYMRKKHPFLTSSDDYCNIAL
                     IAIHSKNLDEDLEYIEKCYEFLNENGFYKGNDLQALSQILLFSDDRTMLKCKKTIELK
                     KEFKENDCKMNYYGYPIIGAISLLDYREDEIIEEIKNVSNKLKEEKGFGNWSLGKSNR
                     VMISSAIVASIYADFIQNENNIGSITNNIFLNIVVAIQIACVMAATSAAIASSSSN"
     gene            complement(5769..6344)
                     /locus_tag="L3M60_002478"
     CDS             complement(5769..6344)
                     /locus_tag="L3M60_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227133.1"
                     /translation="MKLKEALITCVVCSVCLTPLLVNGETKIDADHCKESIVNIDTIV
                     KSGKSFNSKYNQGMEDYKNSDEYKLKVQKEKLQNQLGIKIKKLVPITFEVSYYTSLNQ
                     ENGYGAITATGDDLEDGFVANNHLSFGTKIIVDGKLKTVMDRGSSKYFSNSNAIDVFV
                     PKWSNESESEYYDRVNNMGRHYKKGYIIVEE"
     gene            6661..6924
                     /locus_tag="L3M60_002479"
     CDS             6661..6924
                     /locus_tag="L3M60_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227134.1"
                     /translation="MIKNTKQITVEVNEILLQEISKITNDENNILTDLINESIVNYIL
                     ENKKHNVEDKDLEEVIKFYKKENTEKTKSAARAIAEAKTHTLY"
     gene            complement(7344..7721)
                     /locus_tag="L3M60_002480"
     CDS             complement(7344..7721)
                     /locus_tag="L3M60_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6227135.1"
                     /translation="MTITKIPPAELKVMKFIWISDFTVASKDVIEAMEKLYNWKQTTT
                     LTLLSRLVNKQFLSARKIDRHTHYTVLIGEEEYLHFETKTFLDNMHGSSIESFMKSLF
                     KKGVNEEELNSVEKWVKDIKKDE"
     gene            8062..9174
                     /locus_tag="L3M60_002481"
     CDS             8062..9174
                     /locus_tag="L3M60_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN6227136.1"
                     /translation="MNAFIRKRNKNYVVYLEFEDKETGKRKQKNMGIFDKKREASKRL
                     NEVKESLYKDGFLVPNEITVSEFLLDFLNKYSENISLATYNNYVSICKNYINPSIGKY
                     KIQDLHPIHIQNYIDKLSYKLNPQSIKIHINILKLAIKRAYRLKLIKENVMDGIEAPR
                     YKKFKNEIYDREQMIKLLDLAKNTEMELPINLAIGLGLRISEVLGLTWDNIDFEENTI
                     TVNKITSRINGSVILKEPKTESSIRKISSPKELMSLLKEYKIKQNKNLLKSSIRNNNN
                     LLFFNKKCEPIAEDVISKKFKRFLEKNELPHIRFHDLRHSHVTLLINSKVPIKVISER
                     VGHSNISTTLSVYSHVLKEMDKEASDKISESLFNAN"
     gene            complement(9371..9823)
                     /locus_tag="L3M60_002482"
     CDS             complement(9371..9823)
                     /locus_tag="L3M60_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227137.1"
                     /translation="MVKKRLVAIVMSLAMISVGNFSYAHSGGHNSSGSHHNKNKSELT
                     YYYYCGGHSAHQHNKGFCPYASAKSNSGVTISSAHNEKNHKKFFSEKEYNKGYEDGCK
                     GLYNLSFTEAKSEDSYEKGYLIGYNEYKAKKVYDVIKKMLDIPMLASI"
     gene            complement(10018..11643)
                     /locus_tag="L3M60_002483"
     CDS             complement(10018..11643)
                     /locus_tag="L3M60_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6227138.1"
                     /translation="MLQVTGVGLRFGDKELFKDVNLKFTKGNCYGIIGANGAGKSTFL
                     KILSGEIEPNTGSISITDKERMSVLKQDHFEYEEETVLNVVIRGHERLWNIMKEKDAL
                     YMKEDFNEEDGIKAAELEGEFAELDGWDAETNAEKILMGLGITKDMHYKQMKELVGGE
                     KVKVLLAQSLFGKPEILLMDEPTNHLDFKSINWLNNFIMDLEESIVIIVSHDRHFLNQ
                     ICTNIVDVDFGKIQMYVGNYDFWYESSQLALQLAKDQNKKTEEKIAQLKEFIARFSSN
                     ASKAKQATSRKKQLEKLEVEDIQPSRRRYPYVGFTPAREIGNEVLEVHNLTKTIDGVK
                     VLDNVSFRLDRDDKVVFMGDEIATTALFNIVMGELEPDSGEYKWGVTTSQDYLPKNHN
                     KFFDGVEYSLVDWLRQFSEEKSESFIRGFLGRMLFSGEEALKEAQVISGGEKVRCMLS
                     KLMLSNANVLVLDDPTNHLDLESITSVNKGLEKFPGVLLFTSHDHEFISTIANRIIEI
                     TPNGIMDRKMDFDEYLENKEIQEQLAKMYGKDK"
     gene            complement(11981..13000)
                     /locus_tag="L3M60_002484"
     CDS             complement(11981..13000)
                     /locus_tag="L3M60_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HBN6227139.1"
                     /translation="MKLIKTIDAVGQVLCHDITQIIPGEFKGRKFKKGHIVKEEDIPV
                     LLSLGKDNLYVWEKSEGMVHENEGALFLKELTAGENLDFSEIKEGKIDFIAACDGLLK
                     IDVDALFDLNCVGEIMMATLHNNFIVNKGLKVAGTRVIPLVIDEKKLEDAKKVVGNRK
                     IVNVVPFKPKKVGIVTTGNEVFYSRIVDKFGPVIEEKVKGFGCEVIGQTICPDDKDVI
                     KNAIKEFISQGAELICCTGGMSVDPDDVTPTAIKETGADLVTYGSPILPGAMFLLAYY
                     GEVPIMGIPGCAMYHKTTVFDIVLSRVLIDEKLDKYDIARYGHGGLCMNCDVCTYPAC
                     NFAKI"
     gene            complement(12997..13566)
                     /gene="mocA"
                     /locus_tag="L3M60_002485"
     CDS             complement(12997..13566)
                     /gene="mocA"
                     /locus_tag="L3M60_002485"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBN6227140.1"
                     /translation="MINAVIMASGFARRMGVNKLLLDYKGCSIIEHVFRVVNKIDFHQ
                     VVVVSQYEEVLLLCNKYGFEYIDNKNANVGQSESIKLGVLNSSKCDGYMFFVGDQPFI
                     DNLYIDKIINTFKLDRDFIVIPRYKDICGNPVIFPYGKKEELLALKEDEKGKTILKNS
                     SKIKYVEVPKEMLFDIDTKIDYERIGGKL"
BASE COUNT         4735 a         2168 c         1438 g         5248 t
ORIGIN      
        1 cagacgtttt cttcaaatta atcagatgat ttagtatatc ttgaaccttc ccagaatcct
       61 ttaaatatcc taatgccccg tttatttgtg attttaaatc tatttctact tccatatcta
      121 agtcaacatt tccttttttt atagatatat ttttaagcgt tattgggtaa agataacttt
      181 tttctaaagt aatcttattt ccattaacct caaatggaac ttttttattg tagttactta
      241 atattttttc taacatttta tcacttagct taaattttcc aagtttcaca ttttcaagac
      301 ttacaactaa gtttccctcc tgtaaagtag gtctcaattc aagttcatac tgactattta
      361 tcaaaccaaa taccttatat ggtcctgcta cctttatttt accatttttc atttcaacaa
      421 aattattttc aagttcatta ataccatgtt tctgcatcaa tgtatagatt aaatttttaa
      481 actcttcctc tgaaacagat atttttccaa ccattctaag tggatttttt actacttcca
      541 tagaatttat atatgaacct tttgataaat attcttcatc tataatttta ttattttgtg
      601 aactaacatc atatctttct tttggtgtca agatatatac aacaatacct gcaagtacta
      661 aaacaataac aattaaactt aataaaatct ttgcaacctt tcccataatc gtctccttta
      721 caaatctgtc aacagattta aaataaattt agataactaa cttcacataa tattcttaac
      781 aatatacata ctataataca gtcttttaaa aattaattca actaatctca ttttttgtaa
      841 ataaactgta aaaacatgtt tttagtataa aagttaatat tttgcataat actcttaatt
      901 acacctttat ttttttctaa tcttggatta aaattaatat tatgcaatac agtgtagtgt
      961 tctgtattat aatcaaatta aataggaggt atagaatatg tcaaatgaaa cgaactcatc
     1021 taactttata aaaaatatta ttataaacga cctagaaaca ggaaaacatg acagtataat
     1081 aactcgtttt ccacctgagc caaacggata tttacatatt ggtcatgcaa aaagtatatg
     1141 tcttaatttt ggattagcta aggaatttaa tggaaaagct aatttaagat ttgatgatac
     1201 aaatccatta aaagaggatg ttgaatatgt agaatccata aaagaagatg tcaagtggtt
     1261 aggatttgat tggaacgagt taaattttgc atctaactac tttgatgaaa tgtataagag
     1321 agctttaata ctaataaaaa aaggtaaagc ttatgtatgt gatttgacac aagaagaaat
     1381 gagagaatat cgtggtactc taactgaacc aggaaaagaa agtcctcata gaaacagaac
     1441 gattgaagaa aaccttgatt tatttgaaag aatgaaaaat ggtgaattta aagatggtga
     1501 aaaaacatta agagccaaaa tagatatgtc ttctccaaat ataaatctta gagacccaat
     1561 tatatataga atatctcatt caacgcacca caatacagga gataaatggt gcatatatcc
     1621 tatgtatgct tttgctcatc caattgaaga tgcaattgaa ggaataactc actctatatg
     1681 tactttagag tttgaagacc aaagacctct ttatgattgg tttgttaaag aatgtgagat
     1741 ggaaaacata cctagacaaa tagaatttgc tagacttaat ataaataata ctgtcatgag
     1801 taaaaggaaa ttaaaacaac ttgtagatga aggtattgtt gatggttggg atgatcctcg
     1861 tgttcctaca atatctggaa tcagacgtaa gggatatact gcagaagcat tgcgcaattt
     1921 ctgtagtgaa ataggtgtat caaaagtcaa ctctacagta gatagtcaaa tgcttgatta
     1981 tttcctaaga gagaatttac aaccaaaggc tcctcttact atgggagttt taagaccttt
     2041 aaaacttatt ataactaatt acccagaaaa taagactgag atgcttgaaa tagagaataa
     2101 tgctaaagat gaatctcaag gaaaaagatt agttcctttc tctagagaat tgtatataga
     2161 acaggatgat tttatggaag aacctgttaa gaaatatttt agatttttcc caggaaatga
     2221 agtccgttta aaaggtgctt attttgtaaa atgtactgat gtaattaagg atgaaaatgg
     2281 aaatgtagtt gaaatacatg gtacctatga ccctgagact aagagtggct ctggatttac
     2341 aggacgtaag gtaaaatcta caatacattg ggttgatgct aaatcagcta taccatgtga
     2401 atttagattg tttgaaccat taattcttga tgatttacct gaaaacgagg gcaagcattt
     2461 cttagaacaa ataaatccta attcattgga aatattacaa ggttttgttg aacctacaca
     2521 gataaaagat gctaaacctt ttgataaatt ccaatttgtt agaaatggat tctttagtat
     2581 agataataaa tatacaactg atgaaaaatt tgtatttaat agaatagttc ctctaaaaag
     2641 ttctttcaaa ccggctaaat aatcttacaa tcaaaaaatg aagacttaaa atataaatta
     2701 cttctactat aaagaaactt agttagaagt aattttttat ttcagccttc attatttact
     2761 ttaaatgatt aacttttttc actacagatt cccataactt ctattccatc ttttatacat
     2821 tctatgttaa ggggaaaatc tggtcctttt tctccatcaa tatccgttat aagtgaatct
     2881 gcacattcta tatacacctt tttagtttta aagtatagta tctcatttcc attatattga
     2941 tctaaataat cacctctaag cacacttatc aaaactggta ttgattttgg tattggcatt
     3001 cctttaaaaa taatcacatc cagataccca tcatctattt cagccttata tgctagattt
     3061 atatttcctg ctgttttgcc attaaaaatc aacataagat acatatctcc atcataatac
     3121 atttcctcag aagatacttt tatcttaaat tttctcatat acaatgcttc ttctattccc
     3181 tttatataat aagaaattct tccaaatgaa ttttttaagt ctgtatttat cctttgtgat
     3241 acatctgtaa acataccagc gcttgcaaca tttataaagt acttattatt gactttccct
     3301 atgtctattt tcttgggttt tgaatttatg atttcattta tagcttcttt tatatcaaat
     3361 gaaatttgaa gagcatttga aaaatcattt gcagttcctg taggcaaaat tccaattgga
     3421 atttttatgt ttaattcttt cattgcattt actactaaat ctacagtacc atcaccacct
     3481 gctatcaata tgtgttcata agaactatca acttccttaa atgcattctt tacaggttct
     3541 ttaatagtca atctataggg tatcaataag taattttgtt cttgatataa ttctatgata
     3601 gtgtctaaat ttgataatat ctttttttca cctgaattag gattgtatat aagttttact
     3661 tttttcatta tttctcctta ctcattttat tcttttagta tccaaaatct tcattcaaaa
     3721 aaatcacatg tattctttct ggtgttaatt gtctttttat aagtgtataa ctacagcata
     3781 ttcttcctgg acttgaacag tctatacata taccagtact cttacatcca gtatttgtac
     3841 ttaatctttt acaattggct ggagcagcta ttgtttttgt tctttttatg gcttcatcta
     3901 catctttaac tattttattt actcctgcta taactatcac tttatcaggt ccatatatca
     3961 tggcagcaac tctattacca ttcccatcta gattgtaaag ttctccatcc tctgttattg
     4021 cattagtact tgtaaaataa gcatctgcag aaaatgactt tctgtatatt tctttcattt
     4081 cttctggttt gatatctttt gcatatctgt caagaaaatt atatttttct cttataaaat
     4141 caatcactcc tgtttcaaat aaggtcatag aacctccaca tcctactaga gaaccaactt
     4201 taactatttc ttcaattttt ttaagaagct cttctttatt acttactaaa taccctttca
     4261 tattattatt ttctaaagat tttattgttt tttctattct tttttcatta agccaattta
     4321 aattttgatc cattttaact tcccccttat ttttaatatg tacctaatta tttataaact
     4381 ttattttctt tataagttta ttttagtaaa tctaaaaaaa aatacaatat aaaacttaaa
     4441 ttgttttcca taattttttc caaagtaact tactcaatca aaaaaggaaa gtagatgtac
     4501 tattctactt tccctatatt gtatgtagta ttatatattg cttaaaatct atatattaat
     4561 tatatgtttc atattataga atatcacagt taatttaaat gtaatactat attcttttca
     4621 aacatataaa ttgtattatt aaatttaata tatccaactt cagtaagtct tattaatttg
     4681 atgaagaaga tgctatagct gcacttgtcg ctgccattac acatgcaatt tgaatagcaa
     4741 caacaatatt taaaaatata ttattagtta tagagcctat attattctca ttttgtatga
     4801 aatctgcata aattgaagct acaatagcag aactaatcat tactctatta ctttttccta
     4861 aagaccaatt tccaaagcct ttttcttctt taagtttatt tgaaacattt tttatctctt
     4921 ctattatttc atcttctcta taatctaata gtgaaattgc tcctattatt ggatatccat
     4981 aataattcat tttacaatca ttttctttaa attctttttt gagttcaata gtctttttac
     5041 atttaagcat agtcctatca tcactaaata aaagaatttg ggataatgct tgtaaatcat
     5101 ttcctttata aaaaccattt tcatttagaa attcataaca tttttctatg tattctaaat
     5161 cttcgtctaa attttttgaa tgaattgcaa taagtgctat attacaatag tcatcactag
     5221 aagttagaaa tggatgtttt ttcctcatat agttatatat tattttcatt tttgatatac
     5281 atgtaagata atccattttc tctctatttt cataaactat tgatgctgta agtggtaaat
     5341 atgtatcgcc ccaaaatttt tcacttttaa gttctttgta tatatctaaa atcatcttaa
     5401 aactactttc tggatttgat tcaaatgaaa gtagtgttgc catataaaac atattatatc
     5461 ctctaaaatt agagaataat ccagtatttt ttttaataag ctctattcgt tcttctacta
     5521 gtctaggact tattctctta ttcttaagag taaatgcaag tgcacaacta tgttgtatca
     5581 atcccatttg ccaagaacct ttaacttcat ttaatgcttt gtaattttct attagtaagt
     5641 ttacttttcc atttataaaa gactgttcca tattgccctc ctagtaatat atctcattgt
     5701 attaatagtt ttatatttat tatatatata ttaaatatta caatcaatac atattacttc
     5761 aaagagcatc attcttcaac aataatgtaa ccttttttgt aatgtcttcc catattattg
     5821 actctatcat agtactctga ttcactttca ttactccatt ttggcacaaa tacatctatt
     5881 gcattagaat tgctaaaata cttactagac cccctatcca taacagtttt taattttcca
     5941 tctacaataa tcttagttcc aaaactcaag tgattgtttg ctacaaaacc atcttctaaa
     6001 tcatctcctg tagcagttat cgctccatat ccattttctt gattgagact tgtgtaataa
     6061 ctaacttcaa aagttatagg tactagcttc tttattttaa tacctaactg attttgtaat
     6121 ttctcctttt gtactttcag cttatactca tcactatttt tatagtcttc cataccttga
     6181 ttatacttgg aattaaagct tttacctgac tttactatag tatcgatatt tactatagat
     6241 tctttacaat gatctgcatc tatttttgtt tctccattta ccaataatgg agttaaacat
     6301 acactacata caacacatgt aatcaatgct tctttaagct tcataaattc cccccttatt
     6361 attttgatat attagcatat ttaatattat atgtctacat ttagatttaa ataccacacc
     6421 catttgcttc aagaagttgt cttatattta attcacctta aacacttata aatcctttat
     6481 ctcagaatca tatagaataa agctttttct gttaaatata ttttgttaat cttttaaaac
     6541 ctaaaaccac ttttatttaa ttttctattt tttagaattt aagaattaaa attctataat
     6601 tttaaaacat attgaattta tttttttgag atgatataat ttttttaaga ggtggttgat
     6661 atgataaaaa atacaaaaca aattacagta gaagttaatg aaattttatt acaagaaata
     6721 tctaagataa ctaatgacga aaataatatt ttgacagatt tgataaatga atcaattgta
     6781 aattatattc ttgaaaacaa aaaacacaat gttgaagata aagatttaga agaagtgatt
     6841 aaattttaca aaaaagaaaa tactgaaaaa actaaaagtg cagcaagagc tatagctgaa
     6901 gcaaaaactc atactttgta ttaataaaaa aaagttccta aaaattagga acttttttat
     6961 tttaataatt attaatatat ctcaatattt atcaatataa acatgtttaa tacaatctat
     7021 tgtatactta tcttacccta gaaataatca aaacaaaaat aaaattatgc cctttatatc
     7081 taaaacaact aatttacatg attaatatac aaaaattctg acttttaaat tcgtatacaa
     7141 tatcaaacta aaataatatt aatgacccca atataaatta aattctaaca ggtttgattt
     7201 tacatcaaac ttattgtatt aatattaaat aacaagacaa aaatacttgt aaaaatcaca
     7261 aattttacat aaaactttat atttgattca tttaactaat tattgtaaaa actatttatt
     7321 ttatgataat taactacacc aaattattca tcttttttaa tatctttaac ccatttttca
     7381 acagagttca attcttcttc atttaccccc tttttgaaca aagatttcat aaaactctca
     7441 atagaacttc catgcatatt atctaaaaaa gtttttgttt caaaatgtaa atactcttct
     7501 tcgccaataa gaactgtata atgtgtatgt ctatcaatct ttcttgcact taagaattgc
     7561 ttatttacta atcttgataa aagtgttagt gttgtagttt gcttccaatt atatagtttt
     7621 tccatagctt caatgacatc ttttgatgct actgtaaaat ctgaaatcca aataaatttc
     7681 atgactttta attctgctgg tggtatctta gtaatagtca tatgatttta cactcctctc
     7741 aaacttctac ataattaaat actatattaa tagacttttc gagtcaaagg ttttatttaa
     7801 aatatctcat ccaaatttat taatacaaaa aatttatatt tttatttaaa attaaacata
     7861 caatctatta atataaagat aaatatctat tatataagta taacagatta ttaaattaaa
     7921 attctaatat ttgacataat atatacattt tttaaataaa tatatacaat gtattaaaat
     7981 gtatgcaact ctatttcaaa atcattttgg gatatatata tgttataata aatgtataaa
     8041 attttgattg gtgggttata tatgaatgca tttattagaa aaagaaataa aaactatgtt
     8101 gtttacctgg aatttgaaga taaagagaca ggtaaaagaa aacaaaaaaa tatgggtatt
     8161 tttgataaaa aaagggaagc aagcaaaaga ctaaatgagg taaaagaaag cttatataag
     8221 gacggttttt tagtgccaaa cgagattaca gtgtcagaat ttctattaga ttttctaaat
     8281 aaatatagcg aaaatatatc tttagctaca tacaacaatt acgttagtat ttgtaagaat
     8341 tacattaatc catctattgg aaaatataaa atacaagact tacatcctat tcacatacaa
     8401 aactatatag ataaactctc ttataaacta aatcctcaat caattaagat tcatataaat
     8461 atattgaaac ttgcaataaa aagagcctat agactcaaat taataaaaga aaatgttatg
     8521 gatggtatag aggctccaag atataaaaag tttaaaaatg agatttatga tagggaacaa
     8581 atgataaaat tattagattt agcaaaaaac acagaaatgg aattacctat taatttggct
     8641 attggattag gtcttagaat ttcagaagtt ttaggtctaa cttgggataa tattgatttt
     8701 gaagaaaata caataacagt aaataagata acgtcccgaa ttaatggttc tgttattctt
     8761 aaagaaccaa agacagaaag ctctatcagg aaaatatctt ctcccaaaga attaatgtct
     8821 ttattaaaag aatataaaat aaaacagaat aaaaatttat taaaatcttc cattagaaat
     8881 aataataatt tgttgttttt taataagaaa tgtgaaccta ttgctgaaga tgttataagt
     8941 aaaaaattta aaagattttt agaaaagaat gaattacctc atattagatt ccatgattta
     9001 agacactcac atgttacttt acttataaat tctaaagtac ctataaaggt tatatccgag
     9061 agagtaggac actcaaatat aagtactact ctaagcgttt attctcatgt acttaaagaa
     9121 atggataaag aagcttctga caaaatttct gaaagtttat ttaatgctaa ttaatattaa
     9181 atcaacttag cttagcttat tgagatagat tcattcatat taaatgtatc tatctcaaat
     9241 taataactgc ttaaataatc tgttttattt taatttatca cctaagttat tatacttttt
     9301 tattaaagat tgtactttac aataaaaaag atatttcctt atagaaaata tcttttaaaa
     9361 tactatttta ttatatactt gctaacattg gaatatctaa catcttctta attacatcat
     9421 atactttttt tgctttatat tcattgtatc caattaaata tcctttttca tatgaatctt
     9481 cagatttagc ttctgtgaaa cttaaattat aaagcccttt acatccatct tcataacctt
     9541 tattatactc tttttctgaa aaaaattttt tatgattttt ttcgttatgt gctgaagata
     9601 ttgttactcc agaatttgat tttgcactag cataaggaca aaaaccttta ttgtgttgat
     9661 gtgctgaatg tcctccacaa taataataat atgttaactc tgatttattt ttattatgat
     9721 gactccctga tgaattatgc cctcctgagt gagcatatga aaaatttcct acactaatca
     9781 tagctaaact cattactata gctactaacc ttttcttaac cataatctac tccccctaaa
     9841 atattctata tttttattat agcatctact atattatatt taaattttta gtctacaaaa
     9901 tattattcta ctaataaaca tatttaaata acataaaaaa acctcctaat atttgattaa
     9961 aaacttacat acttatgcat agcatgtctc aaatattaaa aggttttata ctaataatta
    10021 tttatctttt ccatacattt tagctaattg ttcttgtatc tctttatttt ctaaatactc
    10081 atcaaaatcc atttttctat ccattattcc atttggagtt atctctataa ttctatttgc
    10141 tatagtagat ataaactcat ggtcatgaga agtgaataaa agtactcctg ggaacttttc
    10201 aagtccttta tttacagaag ttatactttc taggtctaaa tggttagtag ggtcatctaa
    10261 tacaagtaca tttgcatttg aaagcattag cttactaagc atacatctaa ccttttctcc
    10321 tccagatata acttgagctt cttttaatgc ttcttcacca gagaataaca ttctacctaa
    10381 aaaccctctt ataaagcttt cacttttttc ttcagagaac tgtctaagcc aatcaaccaa
    10441 agaatattct actccgtcaa aaaacttatt atgattcttt ggaagatagt cttgagaagt
    10501 agttactccc catttgtatt ctccactgtc tggttcaagt tctcccataa ctatgttaaa
    10561 aagtgcagta gtagctattt catcacccat aaatactact ttatcatctc tatccaatct
    10621 aaatgacaca ttatcaagaa ccttaacccc atctatagtt ttagttaaat tgtgtacttc
    10681 taaaacttca ttcccaattt ctcttgcagg tgtaaatcct acatatggat atcttcttct
    10741 agatggttgt atatcttcaa cttctaattt ctctaactgt ttctttcttg atgtagcttg
    10801 cttagccttt gaagcgttag aactaaatct agctataaat tcctttaatt gagctatctt
    10861 ttcttcagtc tttttatttt ggtccttagc aagttgtaaa gctaattgac ttgactcata
    10921 ccagaaatca tagtttccaa catacatttg tattttacca aagtctacat caactatatt
    10981 agtacatatt tgatttagga aatgtctatc atgtgatact attataacta tagactcttc
    11041 taaatccatt ataaaattat ttaaccagtt tattgattta aaatccaagt ggtttgtagg
    11101 ttcgtccatt aatagtattt caggcttacc aaaaagcgac tgtgctaaca ataccttaac
    11161 cttttcccca ccaactaact ctttcatttg tttgtaatgc atatctttag ttatgccaag
    11221 acccataagt attttctcag cattagtttc tgcatcccat ccgtcaagtt ctgcaaactc
    11281 accttcgagt tcagcagctt ttataccatc ttcttcatta aagtcttcct tcatatacaa
    11341 tgcatctttt tctttcatta tattccaaag tctctcatga cctcttataa caacatttaa
    11401 aacagtttct tcttcatatt caaagtggtc ctgttttaaa actgacattc tttctttatc
    11461 agttatagat atactacctg tatttggctc tatttctcct gataatattt ttaaaaaagt
    11521 agattttccc gcaccattag ctcctattat tccatagcaa ttacctttag tgaattttaa
    11581 gttgacatct ttaaatagtt ccttatcacc aaatctaagt ccaacacctg taacttgtaa
    11641 cattaatata ttttcctttc tttttactca taatttattt tatcataaaa ctttgataac
    11701 atttttatta tctattatca cgtaaatagt atatttttta taccaaattt gtgaaatatt
    11761 tattaaaaca atactaaaaa ttgtaaattt atgaaaatta acaatctctt atattatact
    11821 tataaagaaa tcaaattgca actttattca ttcttttaca cattgaaaat cagttattat
    11881 aacttttaaa taaatatgct tttcttaaaa catcattcta gttgttttgt ttcatattta
    11941 aaaagagtac ttaatttgta aatactcttt tttaatagct ttatatttta gcaaaattac
    12001 atgcaggata agtacacaca tcacagttca tacaaagacc accatggcca tatctagcta
    12061 tatcatactt atcaagtttt tcatcaatta aaactctaga taaaactata tcaaatactg
    12121 tagttttgtg atacattgca catcctggta tacccataat tggaacttcg ccataataag
    12181 caagtaaaaa catagctcct ggtaatattg gtgaaccata tgtcactaaa tcagctccag
    12241 tttcttttat agctgttggt gtaacatcat ctgggtctac actcatccca ccagtacagc
    12301 atataagttc tgctccttga cttataaatt cttttattgc attctttata acgtctttat
    12361 catctgggca tatagtctgt cctataactt cacaaccaaa accctttact ttttcttcta
    12421 ttactggtcc aaatttatca acaattctgc tataaaatac ttcatttcct gtagtaacaa
    12481 ttcctacttt cttaggttta aaaggcacta catttactat ttttctatta ccaactactt
    12541 tttttgcatc ttctaatttt ttctcatcta taaccaaagg tatcactctt gttccagcca
    12601 cttttaaacc tttatttact ataaaattat tatgaagagt agccatcatt atttctccaa
    12661 cacaattcaa atcaaaaagt gcatcaacat ctatctttaa aagaccatca catgctgcta
    12721 taaaatctat ttttccttct tttatttcac taaaatctaa attttctcca gcagttagtt
    12781 cttttaaaaa caatgcacct tcattttcat gaaccattcc ttctgacttt tcccatacat
    12841 acaaattatc tttaccaaga gataataaca ctggaatatc ttcttctttt actatatgac
    12901 cctttttaaa ttttcttcct ttaaattcgc caggtattat ttgagttatg tcgtgacata
    12961 aaacttgtcc aacagcatct atagttttta ttagtttcat aatttacccc caattctttc
    13021 atagtctatt tttgtatcga tatcaaataa catttcttta ggaacttcta catactttat
    13081 ttttgatgaa ttttttaaaa tagttttacc cttttcatct tctttaagtg ctaacaattc
    13141 ttctttctta ccatatggga atataactgg atttccacat atatctttgt atcttggaat
    13201 tactataaaa tctctatcta atttaaaagt atttataatt ttatctatat ataagttatc
    13261 aataaatggc tggtctccta caaagaacat ataaccatca cattttgagc tatttaaaac
    13321 tcccaatttt atagattcac tttgacctac atttgcattt ttattatcta tatattcaaa
    13381 accatattta ttacacaata ataaaacttc ttcatattga cttactacta caacttgatg
    13441 aaaatcaatc ttattgacta ctctaaatac atgttctatt atagaacatc ctttataatc
    13501 taataaaagt ttatttactc ccattcttct ggcaaaaccg gatgccatta tcactgcatt
    13561 tatcatatat atcatccttg gtatttata
//
LOCUS       DAFNNB010000103        13577 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0104, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000103 DAFNNB010000000
VERSION     DAFNNB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0104"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(218..1885)
                     /locus_tag="L3M60_002486"
     CDS             complement(218..1885)
                     /locus_tag="L3M60_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HBN6227141.1"
                     /translation="MKKINLKIEGKDKEYSLEENSHGIRLGDIAKEFCDEHKGYITLA
                     VVDNKLKELNCRVKKDCEINFLDTTNEDGERVYFRVMSFIFVMACREIFWDSRVTIEH
                     SLSDGLYCEVHIDRKLKEVDVENIKNKMKEIVNNDYVIEKIEVTRNEAISIFENNEMY
                     EKAELLKYKEYDSAKVYKCRNYIDHFYGYMLPSTGYIKSFDIKLYNGGVIILGPSEED
                     KTLPMKFVPQPKLSSVYYEAEEWSRLMGIDKVVSLNKIIENNEYGDIIRTFEALHEKK
                     LSQIADMIKDNNKRVILIAAPSSSGKTSFAHRLSIHLRVNNLNPISISLDDYFIDRKH
                     TPLDEYGNYDFESIYAIDLEKFNLDLKKLLAGEEIDDIRFNFKEGKREYTGKKIKIDS
                     NQPIILEGIHGLNPILTSSIPDEDKFKIYISALTQINLDDHNRIPTADLRMIRRIVRD
                     YNFRGYSADNTILQWASVRRGEKKNIFPYQEEADAIFNSACVYELAVLKRFAKPLLED
                     IKEDNPAHIEATRLLKFLQYFVELDDTSDIPGISILREFIGGSKIVD"
     gene            complement(1959..2714)
                     /locus_tag="L3M60_002487"
     CDS             complement(1959..2714)
                     /locus_tag="L3M60_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HBN6227142.1"
                     /translation="MAVGIASLLFNIEEALNICESIKQITHLEIGIDNISECSDLCKY
                     KERISKLGLSIGIHLPMELNTCENIEYIRNSWINFIEKIEFELRGFDIRYFNLHLGYV
                     MTNRVRRNRDKYLENSVDFLDKLNTNSYVCIENTYSKGGDFSNIGNISYDFEYIFKRI
                     KNSKICFCYDTGHCLIDEDDYVKNLKDKIRLIHLSDNDGINDTHVGIGRGILSEEGIK
                     EVLTLDAEYLVLEINYEDIEDTISKLDNIVGEG"
     gene            complement(2724..3260)
                     /gene="lepB"
                     /locus_tag="L3M60_002488"
     CDS             complement(2724..3260)
                     /gene="lepB"
                     /locus_tag="L3M60_002488"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBN6227143.1"
                     /translation="MNETIKEEIVEWIKIIITALFLAFIITRFIKPTLVNGESMYPTL
                     KSHDYLVANRMTYKLSEPKCGDIMIFKTDLLQENGRKKELVKRVIGVPGDHLKIKDSK
                     VYINGKLLNEVSYIHDNYTEGDIDMVIPKGKVFAMGDNREVSLDSRYKEVGLVDEENI
                     KGKVILRVFPFTDIGIFE"
     gene            complement(3426..3962)
                     /gene="lepB"
                     /locus_tag="L3M60_002489"
     CDS             complement(3426..3962)
                     /gene="lepB"
                     /locus_tag="L3M60_002489"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBN6227144.1"
                     /translation="MGEAVKKEVVEWIKVIVIALVLAFAITRFIVPTIVKGESMYPTL
                     VERDYLIVNRIAYKVGEPKYKDIIVFKTDLTEENGKKKDLVKRVIGVPGDHVKIQDSK
                     VYVNDKLLDETSYIHNNRTDGDVDIVVPEGKLFAMGDNREKSLDSRYDEVGLVDEHAI
                     LGKVLVRLYPFSKIGTID"
     gene            complement(4062..5372)
                     /locus_tag="L3M60_002490"
     CDS             complement(4062..5372)
                     /locus_tag="L3M60_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HBN6227145.1"
                     /translation="MTKLPKEFLEDVEEILKDEYDDFIKSYEESKTTGLRINTLKIDK
                     KKLFDLGLFDLNQISWAEEGFYYDDKIDRPGRNPLHEAGAYYLQEPSAMSVIPKADIK
                     EGEKVLDMCAAPGGKSTYILSKLNDTGLLVSNEINVSRIKALGENLERFGARNCIITN
                     TDSNSLRKTFNGYFDKVIIDAPCSGQGMFRKDEVAISDWSHAKVVECQSIQKEIIRDG
                     YKMLKKDGILIYSTCTFSREENEHVINEFISEYPHAELIEMERLWPHKIKGEGHFVAK
                     IKKLEEEECNVKELKIKNLDKEIKDYREFEKKFLKVDFYSNKKNKFYLRRENLYLLPK
                     IYPDTKKLKVLRYGLHLGVLKKNRFEPSHALSHYLKVEDVKNVENFSVQSESILKYLK
                     GDVVNSNDSRGWVLVSVEGIPLGWGKETSGVIKNHYPKGLRKVF"
     gene            complement(5451..6167)
                     /locus_tag="L3M60_002491"
     CDS             complement(5451..6167)
                     /locus_tag="L3M60_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HBN6227146.1"
                     /translation="MKLDLFLLAIVPILIGMFWIRSKDRYCREPLIHLIKFFLIGAFL
                     SVIIILLENLLMKFNVFEGYSELIYVSFVVAGLVEEGVKALILIPALIKEKHFTEKLD
                     GIIYSVFLALGFATIENMVYIFSESRNLALQVGINRAVISIPAHVMFAITMGYYISKY
                     KFEGNKNKRREYLFMAVLIPILLHGVFDFILMIEYRWAIILLIVYVILLWKINLDKLE
                     KYMNNSKKVFFGNLRKKKKK"
     gene            6404..7675
                     /gene="sleC"
                     /locus_tag="L3M60_002492"
     CDS             6404..7675
                     /gene="sleC"
                     /locus_tag="L3M60_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex-lytic protein SleC"
                     /protein_id="HBN6227147.1"
                     /translation="MQDGFLTVSIIDATNNRPIQNAVVNIYSMSNGSQSSSTLYQNLR
                     SNESGQVTGLVLPAPDVDYSLQPSDVRPYSQYIVEAIADGYETVVIEGTQLLATIEAR
                     QGVPMSPRTRSKRSFSRQSELIFDIGEHTLYGTYPPKIPESNLKPLPPPTGFVVLDNP
                     VVPEFIVVHDGLPEDSSAPNYWIPFKEYIKNIASSEIYSTWPEQTIYANVIAIISFTL
                     NRVFTEWYRNKGYNFTITSTTTYDHKFINNRNLFEPINVVVDAIFNTFIKRPPTSRQP
                     LLAQYCDGQKSQCPDQMTQWGSKDLGDQGYDYESILRFFYGDEIVFERAPIVSGVPVS
                     FPGTTLQVGSSGQYVRTIQNQLNAISNSYPAVPKVIEDGIYGTDTENAVRIFQGIFGL
                     PQSGVVDFKTWYEISRVYVATTRIASLNPLI"
     gene            complement(8149..8805)
                     /locus_tag="L3M60_002493"
     CDS             complement(8149..8805)
                     /locus_tag="L3M60_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227148.1"
                     /translation="MKKGDFIWSGILICIIAVLAIPSSREVFMEATGAHPYIGGFFKF
                     AVLATMGDLLGARILYKDWKIPVGVFYRAIVWGIIGIVTTYGMSIYSSGVGQAQVDGM
                     LPFEGIAFANALFKSATMNILMSPTVFLFHKVMDCFIDKKYEVGKGIKVKLKDVVNIV
                     DWNAYLGFSVLKTIPFFWIPCHTIVFLFPAEYRVIASAFASIALGLILALANKSKVAV
                     "
     gene            complement(10006..10416)
                     /locus_tag="L3M60_002494"
     CDS             complement(10006..10416)
                     /locus_tag="L3M60_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227149.1"
                     /translation="MKIKKSLISAMMVSAMVLGGTGAVFANGNDSSNIALPKLESLIK
                     SGITDPTELEKYGVEHYTYEEYAKHIQELKDYAKDPNAVKDVSQKDLEETIKKMEKDL
                     EKIKTEGLKIMKPITIKNEDGSQISIAYNYAAID"
     gene            complement(10632..11990)
                     /locus_tag="L3M60_002495"
     CDS             complement(10632..11990)
                     /locus_tag="L3M60_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBN6227150.1"
                     /translation="MFIKLLEMSFTSGVLLLIVIFIRACLFKKLPKKVFVLLWLIVLF
                     KLLIPVSLNSGFSIYSIIESTFDVELGNASTENYTLKKAGDSTGKNNSDKFEIMSSIE
                     NIMMLEMLGILSFIVTIIFSILLCFRYIKIINLFKTSIHIKNHEFIKKWMCKNSRFRR
                     FIVCISEFTRTPLTSGILKPRIILPKQMNYANNQTLDFVLTHEMVHINSFDNLLKLFM
                     LVALVVHWFNPLVWVMFFLLNKDIEFSCDEKVMSILGEEYRADYATTLISLAEENNPF
                     SMTICSGFGKTKIEKRIVNIMKFKKSTYVTFLVSTVLVCGIGLVFATENKEINEVESF
                     GLTKNEKSVLSISSDSTELYTPKVDSNISVSMSKVSMNKSSESKSYEDISMNKVRNTE
                     NYSEVSVEKSSKSNDYEDVLYTPSAEYSIQKNNYNEGVLVISRKGNLLAVNNIQYRTS
                     IK"
     gene            complement(12001..12360)
                     /locus_tag="L3M60_002496"
     CDS             complement(12001..12360)
                     /locus_tag="L3M60_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6227151.1"
                     /translation="MEIKLFDSELKVMEVLWERGESKAGELVKILKEETGWNRNTTYT
                     VIKKLVSKGAIERIEPNFVCKALISKEEVQKQETETLINKMFGGSSEKFLSAFINKEN
                     LSSDEITKLKQIVEKLK"
     gene            complement(12565..13344)
                     /locus_tag="L3M60_002497"
     CDS             complement(12565..13344)
                     /locus_tag="L3M60_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HBN6227152.1"
                     /translation="MVSRQELKRVSKAQLSGNWGICAISMAVYIALIVLISIVGLPFE
                     PLTTIVVAVIAFCVSAGFTCMFLKITKDEKVKMGDIFVNGRIYLRSFGFYLFESIIVY
                     IYSFIITIIGAFLSFGVLLTSGAIEYLSSDNVGIGTIISTGITLVLIILVLMLPVYIL
                     LLYVSQATFLICENKDNLGVFKSMSASLDMMKGNVLKLFVLNFSFIGWYILSIATLGI
                     GLLWLIPYVGVTNCNFYRQLVKENPDVEDILLDKEKSYTMY"
BASE COUNT         4631 a         2435 c         1328 g         5183 t
ORIGIN      
        1 aaaattatca cacaaattaa caaaatatta aacaaagaag taaactttaa aataaaaagc
       61 ttgcttcata caaaagtata aaacaagcct ttttatatat caactcaaac tttaaaatct
      121 gtatcaattt aattttccat ttaatatact tcacataaac caatcgcact atttactatg
      181 ccatttaatc ttcataaaat aatctataat acacatatta atcaactatt ttacttcctc
      241 caataaattc cctaagaatt gaaatacctg gtatatcaga agtgtcgtcc aattcaacaa
      301 aatactgcaa aaactttagt aatctagtag cttcaatatg tgcaggatta tcttccttta
      361 tatcttccaa caaaggtttc gcaaatcgtt ttaaaactgc caattcatat acacaagcag
      421 agttaaatat agcatctgcc tcttcttggt atgggaatat attcttcttt tctcctcttc
      481 taactgatgc ccattgtaaa atagtattat cagcactata tcctctaaaa ttatagtctc
      541 taacaattct tcttatcatt ctcaaatcag cagtagggat tcgattatgg tcatccaaat
      601 ttatttgagt aagggcactt atgtatatct taaatttatc ttcatctggt atagatgatg
      661 ttaaaatagg atttagccca tgtatacctt ctaatataat tggctgattt gaatctatct
      721 ttattttttt accagtatac tctctcttgc cttctttaaa attgaatctt atatcatcaa
      781 tttcttcacc agctaaaagt ttctttaagt ctaagttaaa cttctctaaa tctattgcat
      841 aaattgattc aaaatcataa tttccatatt catctaaagg ggtatgtttt ctatctataa
      901 aataatcatc taaagaaatc gaaatagggt ttaaattatt cactctaaga tgtattgata
      961 atctgtgagc aaaacttgtt tttccagatg aagatggagc tgctattaaa attactctct
     1021 tattgttatc ctttatcata tctgctattt gagataactt cttttcatgc aatgcttcaa
     1081 atgttcttat tatatctcca tattcattgt tttctattat cttattaaga gatactacct
     1141 tgtcaattcc cataagtctt gaccattctt ctgcttcata atatacactt gatagcttag
     1201 gttgaggtac aaatttcata ggaagagtct tatcttcttc actaggacct aaaattataa
     1261 cacctccatt ataaagctta atatcaaatg attttatata accagttgat ggcaacatat
     1321 aaccataaaa atggtctata tagtttctac acttatatac tttagcacta tcatactctt
     1381 tgtatttcaa aagctctgcc ttttcataca tttcattatt ttcaaagata gaaatagctt
     1441 catttcttgt aacttcaatt ttttctataa cataatcgtt gtttactatc tctttcatct
     1501 tgttttttat attttcaaca tccacttctt taagctttct atctatatga acttcacaat
     1561 atagcccatc tgataacgaa tgttctattg taactctact atcccaaaag atttctctac
     1621 aagccattac aaatataaac gacatgactc taaagtaaac tctctctcca tcttcattag
     1681 tagtatctaa aaaatttatc tcacaatcct tcttaactct acaatttagc tcttttagtt
     1741 tattgtctac aactgcaagt gttatataac ccttatgctc atcacaaaat tctttggcta
     1801 tatctcccaa tctgatacca tgggaatttt cttctaatga atattcctta tcttttcctt
     1861 ctatttttaa gtttattttt ttcacttcaa catctccttc cttttgatga atataaattt
     1921 taattaatga ggcatatcaa caagataaag agaggtattt aaccctctcc tacaatatta
     1981 tccaatttac ttatagtatc ttcaatatct tcgtaattta tttctaatac taaatattct
     2041 gcatctagtg ttaagacctc tttaatcccc tcttcagata aaattcctct tcctatacca
     2101 acatgtgtat cattgatacc atcattatca cttaagtgta tcagccttat tttatccttt
     2161 aagttcttta cataatcatc ctcatcaatt aaacagtgtc cagtatcgta acaaaaacaa
     2221 atcttagaat ttttaatcct cttaaatata tattcaaaat cataagatat atttccaata
     2281 tttgaaaaat ccccaccttt tgaataagta ttctctatac atacatatga atttgtattt
     2341 agtttgtcta aaaaatccac actattttct aaatatttgt ctctattcct tctaactcta
     2401 tttgtcataa catatcccaa atgaaggtta aaatacctta tatcaaatcc tcttagttca
     2461 aattctatct tctcaataaa atttatccat gaatttcgta tatactctat gttttcgcaa
     2521 gtatttaact ccataggcaa gtgtatccct atagataatc ctaacttaga aattctctct
     2581 ttatatttac atagatcact acattcagat atattgtcaa ttccaatctc taaatgagtt
     2641 atttgcttta tactctcaca aatgtttaaa gcctcctcaa tattaaacaa taaggatgct
     2701 ataccaactg ccatacaaat cacctactca aaaataccta tatctgtaaa aggaaatact
     2761 cttaaaataa cttttccttt aatattttct tcatctacta atcccacttc tttatatcta
     2821 ctgtctaaac taacttctct attatctccc attgcaaata cttttccctt aggaatcacc
     2881 atatcaatat cgccttcagt ataattatca tgtatatatg aaacttcatt taataactta
     2941 ccatttatat aaaccttaga atccttaatt tttagatggt caccaggaac acctataacc
     3001 ctttttacaa gctctttttt ccttccattc tcttgtaata aatcagtctt aaatatcatt
     3061 atatctccac attttggttc tgataactta tatgtcatcc tgtttgctac caaataatca
     3121 tgtgatttaa gtgttgggta cattgattct ccatttacta gtgttggttt tataaaacga
     3181 gttataataa atgccaaaaa aagtgcagta ataattattt ttatccactc tacaatctct
     3241 tctttaatag tttcattcat tcacaaattc tcctttatag ttatattatc tttattaata
     3301 atgtctctta aacactatct tttagataag tctactaaat aaggtgttgt cttttttata
     3361 tagacaacta ttgtgtatgg tctggtgata tcagttaatc agcgattgct ccaaatttta
     3421 tatgtttagt caatagtccc tatcttagaa aatggataca atctgactag aacctttcct
     3481 aaaatggcat gctcgtcgac caatccaacc tcatcgtatc tactatctaa acttttttct
     3541 ctattatctc ccattgcaaa taattttcct tctggaacta cgatatcaac atctccatca
     3601 gtacgattat tatgtatata ggaagtttca tctaacaatt tatcatttac atataccttg
     3661 gagtcttgta tttttacatg gtcaccagga accccgataa ctctttttac taaatctttt
     3721 ttctttccat tttcctctgt taagtcggtt ttgaatacta ttatatcttt gtattttggc
     3781 tctcctacct tgtacgcaat tctattaact atcaaataat cacgttcaac caatgtagga
     3841 tacattgatt ctcctttgac tattgttggc actataaaac gagttattgc aaatgccaaa
     3901 acaagagcta tgacaatcac ttttatccat tctacaactt cttttttaac tgcttcaccc
     3961 actaaaatcg tctcctctgt attaaatatt aagtcaatct acagcaaaat aatctactat
     4021 aaacctacaa tataaatatt atacttcttt tttctactag tttaaaatac ttttctaaga
     4081 cctttaggat aatgattctt aattacacca gaagtttctt ttccccaacc taaaggtatg
     4141 ccttcaactg aaaccaaaac ccatcctcta ctatcattag aatttacaac atcccctttt
     4201 aaatatttaa gaattgattc actctgtaca ctgaagtttt caacattttt tacatcttca
     4261 acttttaagt aatgagatag tgcatgagaa ggctcaaatc tattcttctt taaaacccct
     4321 aaatgcaaac cgtatctcaa tacttttagt ttttttgtat ctggatatat tttaggaagt
     4381 aggtacaaat tttctcttct taaataaaat ttattttttt tattactata aaaatcaacc
     4441 tttaaaaatt tcttttcaaa ctccctataa tcctttattt ctttatctaa attttttatt
     4501 tttaattctt tgacattaca ttcttcttct tctaattttt ttattttggc aacaaaatgc
     4561 ccttctcctt ttatcttatg aggccataat ctttccattt caataagttc tgcatgtgga
     4621 tattcactga taaattcatt tataacatgc tcattttctt ctcttgaaaa agtacaagtt
     4681 gagtaaatta aaataccatc cttctttaac attttatatc catctcttat aatctctttt
     4741 tggatggact gacactctac tacctttgca tgactccaat ctgatatagc aacctcgtcc
     4801 ttcctaaaca taccttgccc agaacaaggt gcatctataa taaccttatc aaaataccca
     4861 ttaaacgtct ttcttaagct attagaatca gtgtttgtta taatacaatt tctcgctcca
     4921 aatcgctcta gattttcccc tagagcctta attctactaa cattaatttc attagaaacc
     4981 aataaacctg tatcatttag cttagatagt atatatgtag attttccacc aggagcagca
     5041 cacatatcga gaactttttc accttcttta atatctgcct ttggtattac actcattgca
     5101 gaaggctctt gcaaatagta tgctccagcc tcatgcagtg gatttctacc aggtctatca
     5161 attttatcat cataataaaa accttcttct gcccatgata tttgattcaa atcaaacaga
     5221 cccaaatcaa ataatttttt cttgtcaatt tttagagtat ttattctaag cccagtagtc
     5281 ttactttctt cataactttt tataaaatca tcatattcat cttttagtat ttcttcaaca
     5341 tcttctaaaa attcttttgg taactttgtc aaatctataa tcccccttca atctgcttta
     5401 cttgtgtgtc atttattata ctttaattat ttatttcttt ctcttaaatt ttatttcttc
     5461 ttctttttcc taagattacc gaaaaacact tttttagagt tattcatata tttctcaagt
     5521 ttatcaagat ttatcttcca caaaagtatt acataaacta taagtaatat aatagcccat
     5581 ctatattcta tcatcaatat gaaatcaaaa actccatgta ataaaattgg aattaacact
     5641 gccataaaca aatactcacg tcttttattt ttatttccct caaatttata cttagaaata
     5701 tagtaaccca tagtaattgc aaacattaca tgagctggta tagagatgac tgctctatta
     5761 ataccaactt gcagtgctag atttctactt tcagaaaaaa tataaaccat attttcaatt
     5821 gttgcaaatc ctaaagctaa aaatacagag taaataatac catctaactt ttcagtaaaa
     5881 tgtttttctt taattaaggc aggaatcagt atcagagctt ttaccccctc ttctacaagt
     5941 cctgcaacaa caaatgatac atatattagt tctgaataac cttcaaatac attaaacttc
     6001 atcagtaaat tttctaaaag tataattata acacttagaa aagcaccgat taaaaaaaat
     6061 ttaattagat gtattagagg ttctctacaa tatctatctt tagaccttat ccaaaacatt
     6121 cctatcaata taggaactat tgctaataaa aataaatcta gcttcataga ttattttcct
     6181 ttcaaaaatt ttgatttatt tatgacttat atcatctact tatttttaac ttatttatgt
     6241 tttaaataca cactaaaata tacattaaat tattttaaat tatttaaata caaatatact
     6301 caaaaaaatt taaatttata tctatattat ttaacaaatt tcaagctata ttcatatctt
     6361 gaaatataag ggaactaaat tcatttaaag aaagggtgat ttgatgcaag atggtttctt
     6421 aacagtaagc ataattgatg ctactaacaa tagacctatt caaaatgcag tagtaaacat
     6481 atattcaatg tctaatggta gccaaagctc atcaacttta taccaaaacc taagaagtaa
     6541 tgaatctggt caagtgactg ggttagtatt accagcacca gatgtagatt actctctaca
     6601 accatctgat gttagacctt acagccagta tatagtggaa gccatagcag atggatatga
     6661 gacagttgtc atagagggaa cacagttatt agctacaatt gaagcaagac aaggagttcc
     6721 catgtctcct agaacaagaa gtaaacgttc tttctcaaga caaagtgagt tgatttttga
     6781 cataggagaa catactttat atggtactta tcctcctaag attccagaaa gtaacttaaa
     6841 accattacca cctcctactg gtttcgttgt attagataac cctgtagtgc ctgaatttat
     6901 agtagttcac gatggtttac cagaagattc atcagctcca aactattgga ttccatttaa
     6961 agagtacatt aaaaacatag catcatctga aatatattca acttggcctg agcagacaat
     7021 ctatgcaaat gtaatagcta taatatcatt tactttaaat agagtcttta ctgaatggta
     7081 tagaaataaa ggatacaact ttacaataac ttcaacaaca acatatgacc ataaatttat
     7141 aaataataga aacttatttg aaccaattaa cgtagtcgta gatgctattt ttaatacctt
     7201 tataaaaaga cctcctacat caagacagcc actattagca caatattgtg atggacaaaa
     7261 atcccaatgt cctgaccaaa tgactcaatg gggcagtaaa gatttaggtg accaaggata
     7321 tgactatgag agtatactaa gatttttcta tggagatgag atagtgtttg aaagagcacc
     7381 tatagtaagt ggtgtaccag tatcattccc tggtactaca ctacaagttg gttcttctgg
     7441 gcagtatgtt agaactatac aaaatcagtt aaatgccata tctaattctt atccagctgt
     7501 cccaaaagtt atagaagatg gtatctatgg aacagataca gaaaatgctg ttaggatatt
     7561 ccaaggcatc tttggtttac ctcaaagcgg agtagtagat tttaaaacat ggtatgaaat
     7621 atccagggtt tatgtagcta caactagaat agcttcttta aatcctttaa tttaaagctt
     7681 gatttagaat ataaactcaa attattaaat aaaattatta acttaaaata aaaatggctg
     7741 ttatgaacta acatatacca taagtatttt tacttataag tattgtagtc ttaacagcca
     7801 ttttttagtt ataacttatt tttttagaac cttaaaactt taaatggggg attttaagct
     7861 tcttaaaaac agtgcttagc aactttaatt tctttaaaaa tcttatcttt atttttaaat
     7921 ttaataaaat gctattttaa tgaaaagctt actttttact tagagcctca attcttattt
     7981 aaaatgctac ttctcattta gaatattact ccttattatt taaaacatta tttctcactt
     8041 agaatattac ttcccatcta aattattatt tattcattat aaatataaac actattaatt
     8101 taaaaagtta ttcctaattt taataatacc atttccttat caatagacct aaaccgcaac
     8161 tttagactta ttagctaaag ccaatattag acctaaagct atagatgcaa atgcagaagc
     8221 aattactcta tactcagcag ggaataagaa tacaattgta tgacatggta tccagaaaaa
     8281 tggtatagtt tttaaaacac taaaaccaag ataagcattc cagtccacaa tatttaccac
     8341 atctttaagt tttaccttaa taccctttcc aacttcatat tttttatcta taaaacaatc
     8401 cataacctta tggaataaaa atacagttgg tgacattagt atattcatag ttgcgctctt
     8461 aaatagagca tttgcaaaag ctattccctc aaatggaagc ataccatcca cttgtgcttg
     8521 acctacacct gatgagtaaa ttgacattcc atatgtggta actataccta ttattcccca
     8581 aactattgct ctataaaata ctcctactgg tattttccag tccttataca aaattctagc
     8641 accaagtaaa tctcccattg tagctaatac agcaaactta aaaaaacctc caatatatgg
     8701 atgtgcacct gttgcttcca taaatacttc tctactactt ggtattgcta agacagcaat
     8761 aatgcaaata agaattccac tccatataaa atcgcctttt ttcatattat aaatactatt
     8821 taatatataa atagttctcc tttctattaa agatttattt aatttacaat ctatctacaa
     8881 ttatttatta atcactgatt attagatatt cattttccat gtttaaattt attctagccc
     8941 tttatctatt tattttctta ctttttttat ccaccactat ctatatattt attttacgat
     9001 tactctttca ccctaaccct ttaactatct atttgttttt tactactctt atctaccagt
     9061 atctatatat ctactttaca agtactcttt caccctaact ctttaactat ctatttgttt
     9121 tttactattc ttatctatca gtatctatat atctacttta caagtactct ttcaccctaa
     9181 ctctttaact atctatttgt ttttttacta ttcttatcta ccagtatcta tatctacttt
     9241 acaagtactc tttcacccta actctttaac tatctatttg ttttttacta ttcttatcta
     9301 ccagtatcta tatatctact ttacaagtac tcttttaacc caacctccta tactttatat
     9361 agtaatatgt ttttaacttt tttcattatt ctcaatatgt tttataacta caaatactct
     9421 ccctccttaa aaataagaat aaaattaacc aacaaattta taatcatatt attactttat
     9481 gtattaagca attactatgc caataaattt taattttttt aaacaactct taaaaatgct
     9541 aaaatgctaa ttttctttaa cttttaatga atctaataga taacatttaa tttttgttat
     9601 atttttcaga atgtttcaat tattattttt aagataaaaa aataatgtta acatttctta
     9661 acatacatta aaaaatgtta acattataaa ttgggtttaa tacttttact ttgactcgat
     9721 ttacttttat tctacttctt tactttattt taacttagac tttatttttt tatcacttat
     9781 ttgagtctta tttattttat tctcattcta tatgttcaat taaatttgtc taagttcttt
     9841 gatattttta ttacataatt ttaacaagct taagtgttat ttgggtagtg caattgataa
     9901 ttatttaaat aatcatcaat tgcatgtttg tatcatcata gtagcttcat gtatttaatc
     9961 atgtttactt aggattttga aatatcttag agattatata taacattagt caattgctgc
    10021 ataattgtaa gcaattgaaa tttgagaccc atcttcattt tttatagtga taggcttcat
    10081 aatttttaat ccttcagttt ttatcttttc taaatctttt tccattttct taattgtttc
    10141 ctcaagatct ttttgactta catctttaac cgcattaggg tctttagcat aatcttttaa
    10201 ttcttgaata tgtttagcat attcttcata agtataatgc tcaacaccat atttttctaa
    10261 ttcagtaggg tcagttattc ctgattttat tagagattca agtttaggta aagcaatatt
    10321 agatgaatca tttccatttg caaatactgc tccagttcct cctagtacca ttgcagatac
    10381 catcattgca cttataaggg atttttttat tttcataacc aatcttcctt tcttatatat
    10441 ttatttggat agtgtagttt aaaacgacta ttgcaatagt tatcaaccac acatttagac
    10501 taccacaata gtcatattta tgtcaagtat ttcactaaaa ttttttaata aaaaatacta
    10561 cctcaaaaca aacttaatta ttttaagata gtattttaat tctaattgct ttgtgctatt
    10621 atgatttgca cttactttat agaagttctg tattgtatat tattaactgc taacaaattt
    10681 ccttttcttg atataactaa aacaccctca ttataattat ttttctgaat tgaatattct
    10741 gctgaaggag tataaagtac atcttcataa tcattagatt tgcttgattt ctctacagat
    10801 acttctgaat aattctcagt attccttact ttattcatag atatatcttc ataactttta
    10861 gattcacttg atttattcat agatactttt gacatactaa cagagatatt tgaatctact
    10921 tttggagtat ataactcagt tgagtcacta cttattgaaa gtacactttt ctcatttttt
    10981 gttaaaccaa aagattcaac ctcattaatc tctttatttt ctgttgcaaa aacaagacct
    11041 atcccacaaa ctaatacagt tgaaactaaa aaagtaacat aagttgattt tttaaatttc
    11101 ataatattaa ctattctctt ttctattttt gtctttccaa acccactaca tattgtcatt
    11161 gaaaatggat tattctcctc tgctaaactt attagtgtag ttgcataatc agccctatat
    11221 tcctcaccaa gtatactcat aaccttttca tcacaactaa attctatgtc tttatttagt
    11281 aaaaaaaaca tcacccaaac taagggatta aaccaatgaa caacaagagc tacaagcata
    11341 aacagcttca acagattatc aaaactatta atatgaacca tttcatgagt taaaacaaag
    11401 tctaaagttt gattattagc ataattcatt tgcttcggca aaataattcg tggttttaaa
    11461 ataccagatg taagtggcgt tctagtaaat tcagatatac aaacaataaa tcttctaaaa
    11521 cggctattct tacacatcca ttttttaatg aactcgtgat tttttatatg aattgaagtt
    11581 ttaaaaagat taattatttt aatatatcta aaacataaaa gtatgctaaa tataatagta
    11641 actataaaag acaaaatgcc caacatttcc aacatcataa tattttcaat actagacatt
    11701 atttcaaatt tgtcagaatt gtttttacct gtagagtcac cagctttctt aagagtataa
    11761 ttttccgttg atgcattccc caattctaca tcaaaagtcg attcaattat actataaata
    11821 ctaaaaccag aatttaaact tactggtatt agaagtttaa ataaaacaat caaccacaat
    11881 aatacaaaca cctttttagg taatttttta aataaacatg ctcttataaa aataactatt
    11941 aaaagtaata ctccagaagt aaaactcatt tctaacaatt taatgaacat aaatcttacc
    12001 tcactttaat ttttcaacaa tctgtttaag ttttgtaatc tcatcacttg aaagattttc
    12061 tttgttaata aaagctgaaa gaaatttttc tgaagaccct ccaaacattt tattaattaa
    12121 agtttctgtt tcctgttttt gtacttcttc cttgcttatt aaagctttac aaacaaaatt
    12181 cggctcaatt ctctctattg caccttttga caccaatttt tttataaccg tatacgtagt
    12241 gtttctattc catcctgttt cttcttttaa aatttttact aactctccag ccttgctttc
    12301 tcctctctcc catagaactt ccattacttt aagttctgaa tcaaataatt ttatctccat
    12361 ataaaatcgc tcctcaaata tagttatcac aatagtcatt actataataa aaaactatca
    12421 tagtagtcta tgctatgtca atcctttatt ttttaataat aaaaactgcc ttttaaaaaa
    12481 tttcttataa aagacagttt tatcaatgtt atactttgat gtttaatcaa agccatattt
    12541 tatttgaatg tataactatc tatcttaata catagtataa gatttttctt tatctaatag
    12601 tatatcctct acgtctgggt tttcttttac taattgtcta taaaagttac aattagtaac
    12661 acctacatat ggaattaacc atagtagacc tattccaaga gtagctattg aaagtatata
    12721 ccatcctata aaactaaagt ttagtacaaa taatttaagt acatttcctt tcatcatatc
    12781 taaactagca ctcattgatt taaatacgcc cagattatcc ttgttttcac aaattaggaa
    12841 agtagcttga gaaacatata atagcaatat ataaactggt aacataagaa cgagtattat
    12901 aagaactaga gttattccag tagaaataat agttcctatt ccaacattgt cacttgataa
    12961 atattcaatt gcaccactag taagtaaaac tccaaatgat aaaaatgctc ctataattgt
    13021 gattataaaa gaataaatat aaacaataat tgattcaaat aaataaaaac caaaacttct
    13081 taaataaatt ctcccattta caaaaatatc tcccatcttg actttttcat ctttagtaat
    13141 tttcaagaac atacatgtga atcctgctga aacacagaaa gctattacag ccactacaat
    13201 agtagtcaat ggttcaaaag gtaatcctac aattgatatt aacactatca atgcaatata
    13261 tactgccata gaaattgcac atatacccca gtttccagac aactgagctt tagatactct
    13321 ttttaattct tgccttgata ccataaaaaa cacccctttc attttataaa aaaactttta
    13381 aaaatataaa taatctttta acatataata ttaatatatc atattttcga ctatataaca
    13441 tacaattatt caccatgttt tatcaagata tgtcataatt gtgttactag ttttatatag
    13501 tcaatattac caagtacatc aaataaattt gactaattat acgacaaata ataaaataca
    13561 ttatactaat tttactt
//
LOCUS       DAFNNB010000104        13573 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0105, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000104 DAFNNB010000000
VERSION     DAFNNB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0105"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            417..1493
                     /locus_tag="L3M60_002498"
     CDS             417..1493
                     /locus_tag="L3M60_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBN6227153.1"
                     /translation="MKTQRLNAVLEQMKKDDISQMLVSDPTSIFYLTGVLIHPGERLL
                     ALYLNLNGNNKLFINELFPVSEDLGVEMVWFNDTQNPVEIITEHIDKNATMGVDKNWP
                     ARFLLNLIELGGGSKFVNSSYIIDTLRMCKDEEEKELMRIASKLNDKAMEQLKATVSG
                     ELTEKQLVGKLSKIYEDLGTDGFSFDPIIGFGPNGANPHGEPGNALVKPGDAIILDIG
                     CIKDNYCADMTRTVFYKEIPEKGREIFEIVLEANKRAEAIVKPGVRFCDIDAAARDYI
                     TEKGYGQYFTHRTGHSIGLEVHDKGDVSSINTDTVQPGMIFSIEPGIYLPGEFGVRIE
                     DLVLVTEDGCEILNKHDKEICVVG"
     gene            complement(1596..2438)
                     /locus_tag="L3M60_002499"
     CDS             complement(1596..2438)
                     /locus_tag="L3M60_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBN6227154.1"
                     /translation="MDKIKLIVDSACDLPDDIIEKYNIEVVGLNVSFGEESYISGKEI
                     DNKTFYRKMSESKVLPKTSCPSPDKFLEAYHCQEESVLVINISSGLSGTYNSALLAKD
                     MFEKEGNNKKIEVIDSLSGSIGIGQLVLKAAILIEEGKEIEEIVSIINKYKDNPPFFG
                     TLETLENAIKGGRINPIAGKIINTLNFKAIIQVADGVVTPIDKARGEGNSLKKLITLV
                     ESKIKDKEEKVLFIGHANCPEKAQKVREVMEKDVKYKDVVICEVGSVMGTYTSKGAIL
                     ITAI"
     gene            2622..3194
                     /locus_tag="L3M60_002500"
     CDS             2622..3194
                     /locus_tag="L3M60_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227155.1"
                     /translation="MKTNNMKQAILSSATNLIAKNGVQNTSLADIAKDVNISKGTLYY
                     HYASKNDIIYDIADTHLEVITSAILNCVKNVKSKNSQIDMVNLILEKISTIESRGRVH
                     MYLICEAITSNNDLKERMRLKYIEWRTTLKSEIVESLEKYNSSENEQDAESFSFLLMS
                     IVDGLVVQSLLKTEKIPYENIASFLINHWI"
     gene            complement(3357..3629)
                     /locus_tag="L3M60_002501"
     CDS             complement(3357..3629)
                     /locus_tag="L3M60_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227156.1"
                     /translation="MLRERFVVRNHHNPHTSECCGTGGKGCCGGDNNEHSHQHSHSHG
                     HEHEHINGVGHDHNHDDGFSGTHEHSHEHTHTHGHEHSDEHTHEHK"
     gene            complement(3743..4615)
                     /locus_tag="L3M60_002502"
     CDS             complement(3743..4615)
                     /locus_tag="L3M60_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HBN6227157.1"
                     /translation="MYGESKKLSIDNNIFLQYCLIILGMILSTLGINLYLAPAKLLSG
                     GVAGICVILYKLFGINQGISSFLMNIPIFIIARKYFDNKFLLISFVNMLLFSLALGLT
                     QDIAKYFPIDDTMLQCIYGGALTGIGMGLTFKARATAGGLDIVAAIMKRKYDIPMKNT
                     FLFINFFVVCAGAFLFGAKLVMYTLITMYIISFTMDIGKDCFDRKKSILLISNKYDEI
                     SKVIMNKMGRGVTFLEAEGAYTQNKKKMIYCIISANEIGKFKDLIYDMDSEAFISVNN
                     VEEVKGGGFKDKFL"
     gene            complement(4902..8009)
                     /locus_tag="L3M60_002503"
     CDS             complement(4902..8009)
                     /locus_tag="L3M60_002503"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBN6227158.1"
                     /translation="MAKFKPLVDSSVKQAEAQVFDYWKDINILEKTLEKGKDDPSFVF
                     YEGPPTANGNPGVHHVLSRTLKDSVCRYKTMSGYQVKRKAGWDTHGLPVEIQVEKELG
                     LTSKQQIEEYGIAEFNQKCRESVFSFEKQWRIMTERMAYEVDLDNPYITLDNNYIESV
                     WWILNKFNKEGYIYEGHKILPYCPRCGTGLASHEVAQGYKEVKTNTVIAKFKKKDADE
                     YFLAWTTTPWTLPSNVSLTVNADVDYLKVKKGDEIYYVSKPLADKVLGEDYEVLEEMK
                     GKDLEGLEYEQLMPFVEVDKKAFFITVGDYVTTEDGTGIVHTAPAFGEDDYNTGKRYD
                     LPVIQPVDETGKFTSTPWEGRFVMEDGLDVEIIKWLASENKLYSKEKVVHNYPHCWRC
                     QTPLVYYAKPSWYIEMTKLKDKLIENNNGVKWFPSFVGEKRFGNWLENLNDWAISRTR
                     YWGTPLPIWRCECGHTDSVGSRAELAERAIEDVNPETVELHRPYVDDIHLKCEKCGKP
                     MTRVTEVIDCWFDSGAMPFAQHHYPFENKENFDQLFPADYISEGIDQTRGWFYSLLAV
                     STFVLGKAPYKSVLVPDLVLDKDGKKMSKSRGNTVNPMELFDQYGADALRWYLLYVSP
                     PWTPTKFDMDGLKEIQSKFIGTMKNVYNFFTLYANTDDINPTEFFVEYKDRPELDRWI
                     LSKFNNLMKDVEENLAIFELNKTLRMIQDFINDDLSNWYIRRSRRRFWATELTEDKKA
                     VYNTTYELLHGLCRAIAPFAPYMSEEMYRNLTGEVSVHLAEYPKCNEELVDTKLEEKM
                     DLAKNLVTLGRASREVERIKVRQPLQKVLVDGKFEDTISDVVDLIKEELNVKEVIFAK
                     DLDEYMNFSLKPNFKEAGPLLGSKMNLFVGALSKLNAHETANKLEKGETLTVDLDGEA
                     FEFNKDLVLIGITAKEGFNVSVENNLFVILDTKLTEELINEGYAREFISKVQQLRKSN
                     NFEVLDNIVIEYCGDDEIAKAVDHFNEYIKSETLALEINRVDDKSLEEQNLNDHMTGI
                     KVVKK"
     misc_binding    complement(8101..8333)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8508..9035)
                     /locus_tag="L3M60_002504"
     CDS             complement(8508..9035)
                     /locus_tag="L3M60_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HBN6227159.1"
                     /translation="MLTPIEIENKEFKKGLRGYRDEEVDEFLDIIKEDYESLCKENTA
                     LREKLGLYQDQVNKYENIEETLKATLITAQSAAEDTCSAANKKAKIIVEEADLKARQI
                     IEQANNRVIEIRKEYDALVKEFKIFRNKFKSLLEDEIRSVDEIFYDVDEKCVNAFEGT
                     TVYNYSNDEAATTLE"
     gene            complement(9070..9855)
                     /locus_tag="L3M60_002505"
     CDS             complement(9070..9855)
                     /locus_tag="L3M60_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HBN6227160.1"
                     /translation="MDKLKLTNHIKDIDLKNKMFKVIDKANSCIKNYDVKSTEFLNPY
                     EVKNAVAILNSTNEIKYSVDGGYEQAERSTVFIYPFYMEYEDIEDTLRFLQIEGNFKF
                     KNISHKDYLGSILGLGIKREKIGDIIIHDSFCQVVVSSDICDFIIVNLEKVSRNNVIV
                     KEISRENIVNSSSKYKEVSFTVSSDRLDCVISGIYNISRQDSAKYINGEKVHVNYEKI
                     TSTSKIVKNDDLISIRGKGRAKVTQIGDITRKGKIKVQARLIV"
     gene            complement(9865..10122)
                     /locus_tag="L3M60_002506"
     CDS             complement(9865..10122)
                     /locus_tag="L3M60_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HBN6227161.1"
                     /translation="MGTIRIALYYLLDIIAWMIVIKSLMTWFPNGRQSKIFDILENLT
                     EPIEGPIRSIMYKYTNGPIDFSPLIAIVLLMFLRQLVLVIF"
     gene            complement(10141..10599)
                     /locus_tag="L3M60_002507"
     CDS             complement(10141..10599)
                     /locus_tag="L3M60_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HBN6227162.1"
                     /translation="MSNGIISKFKNWIVDEEEDYIEDEYESGMDDIVQEEEMNSGFST
                     AKANKIVNLHTTSQMKVVIVEPKVYDEAATIADHLKQRRAVIVNLEGLTNSEVRKSIF
                     NFMNGAVYVLDGSIQKVSKSIFILAPNNVDIDANMKKELESKAFFPWQNK"
     gene            complement(10638..11342)
                     /locus_tag="L3M60_002508"
     CDS             complement(10638..11342)
                     /locus_tag="L3M60_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HBN6227163.1"
                     /translation="MGSIKDNLSEIRSKIDKAAKKVDRDSKEITLLAVTKTVDVDLVN
                     EAMREGITSVGENKPQELARKYDIIGNKLNWHLIGTLQTNKVKYIIDKVCMIHSLDRI
                     ALCEEIQKRAEQINREIDCLVQVNISKEESKHGLDREEVVDFVKEVSEKYKNIKIKGL
                     MTMAPFIEDEDEIRGVFKGLKDLSKQIKELNIPNVGMDVLSMGMSHDYEIAIEEGATI
                     VRVGTSIFGERNYDKK"
     gene            complement(11352..11987)
                     /locus_tag="L3M60_002509"
     CDS             complement(11352..11987)
                     /locus_tag="L3M60_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227164.1"
                     /translation="MKKVKYTRLVILGIFIYLVFNILVMIISKNVDTLVLKNEKVDAK
                     IHVKGAIEKDANDQSNYLSTYISKEDSNNFEKNQNVIIEYREQKIDAKVYKIYENNNK
                     IMVKLKISNQIVANQDTSVEEFDIIYNQMECLKIPKTSIKTKDNKRGVYVINEQSQSV
                     KFVILEGITYENESSVFVDYYKNDLNGVTSVNLYDKIILRPNSINTNIRIK"
     gene            12367..13407
                     /locus_tag="L3M60_002510"
     CDS             12367..13407
                     /locus_tag="L3M60_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6227165.1"
                     /translation="MSEILLGTGLLLLVLCCFTIFSYKAPYGMKAMGALANAACATFL
                     VEAFNLSLLGDVLGMEFFRSVGAANGSLGGVAAAILVPLALGVSPVYAVLIGLSCLDF
                     SILSGFVAGYFVSFLIKFIEKKVPPGVDLVVVILLCAPLSRLIASNVDPLVNSTLLQI
                     GQVLIEATNISPIIMGIILGGLITVVGTSPLSSMALTSIIGLTGLPMAIGALGAFGSA
                     FMNSTLFAKLKLGSKKDIISVAIEPLTQSDIISANPIPIYSTNFFGGAFSGIIVALMG
                     LVNMTPGTATPVAGFAIMFAYNPVQQVLITAGLCALFNVIAGFLGSKIFSTFKVLTAG
                     EIRGTDVEEEIA"
BASE COUNT         4359 a         2276 c         1579 g         5359 t
ORIGIN      
        1 gatatttttc aaaatatttt catgttcaaa aacaatttta ataataaatt ttaaatactt
       61 tgaaatcaaa tcttattaaa aatgataaat ttaaaagttt taccacttta agtctaatga
      121 tactaaattt tttaaattgt ttcaatatta ttttattata aaattttatt tattattttt
      181 catggttata taaaaagcta aaatacataa ctttatagac ttaatttatt acttttatag
      241 aattttcaca ataaaaagtc attttacttc tgtatttaat gcaaaaacag agattaaact
      301 taatataaaa aatatataac aaaatttttt tatatttcta aaagaaaagt atactctttt
      361 ttgtttaata atgctaaaat tgatttgtat tgaattttga ttaggaggaa attattatga
      421 aaactcaaag attaaatgct gtacttgaac aaatgaaaaa agatgatatt tctcaaatgt
      481 tagtaagtga ccctacttcg atattttatc ttacaggggt actaatccat ccaggtgaaa
      541 gattactagc attatactta aatttaaatg gcaataataa attatttata aatgaattat
      601 tcccagtttc agaagattta ggtgttgaaa tggtttggtt taatgatact caaaatccag
      661 tagaaataat aactgaacat atagacaaaa atgctactat gggtgttgat aaaaactggc
      721 cagctagatt cttattaaac ttaatagaat taggtggagg aagtaagttt gtaaattcat
      781 cttatataat agatacttta agaatgtgta aagatgaaga agaaaaagag ttaatgagaa
      841 tagcttcaaa attaaatgac aaggctatgg aacaacttaa agctactgtt tctggtgaac
      901 ttacagagaa acaacttgtt ggtaaattat ctaaaatata tgaagaccta ggtactgatg
      961 gattttcatt tgaccctatt ataggttttg gtcctaatgg agctaatcca catggtgaac
     1021 ctggaaatgc acttgtgaaa ccaggagatg caataatatt agatataggt tgtataaaag
     1081 ataactattg tgctgatatg acaagaactg tattctataa agaaatacct gaaaaaggta
     1141 gagaaatatt tgaaatagtt cttgaagcta ataagagagc tgaagccatt gtaaaaccag
     1201 gagttagatt ctgtgatata gatgcagctg ctcgtgatta cataactgaa aagggatatg
     1261 gacaatactt tactcataga actggtcact ctatagggct tgaagttcat gataaaggag
     1321 atgtatcttc tataaataca gatactgtac aaccaggaat gatattctct atagaaccag
     1381 gtatatattt accaggtgag tttggagtta gaatagaaga tttagtactt gttactgaag
     1441 atggttgtga gatattaaac aaacacgaca aagaaatatg tgtagttggt taattctaaa
     1501 atcaatttga taaaattaaa aaaccatata aaaatgttta aaacattttt atatggtttt
     1561 aatttctcaa tctatgattt atttcaatcc atcatttata ttgcagttat taatattgca
     1621 ccttttgaag tatatgtacc catcactgaa ccaacttcac atattacaac atctttatat
     1681 tttacatcct tttccataac ttcccttacc ttttgagcct tttcagggca attagcatgt
     1741 cctataaata aaaccttttc ttctttatct ttaatttttg actctactaa agtaatcagc
     1801 tttttcaaac tatttccttc gcctcttgcc ttatctatag gagttacaac gccatctgca
     1861 acttgtatta tcgccttaaa attcaaggta tttataattt ttcctgctat tggatttatc
     1921 ctaccaccct taatagcatt ttctaaagtt tcaagagttc caaaaaatgg aggattatct
     1981 ttatatttat ttattattga aacaatctct tcaatttctt ttccttcttc tattaatatt
     2041 gccgctttta acactaattg tcctattcca attgacccac taagtgaatc tatgacctca
     2101 attttcttgt tatttccttc tttttcaaac atatcttttg ctaaaagtgc actattataa
     2161 gtcccagata atcctgaact tatattaatt accaatacac tttcttcctg acaatggtat
     2221 gcttctaaaa atttatctgg agaaggacat gatgtttttg gtagtacttt gctctcactc
     2281 atttttctat agaatgtttt attatctatt tcctttccag atatataact ttcttctcca
     2341 aaagatacat ttaaaccaac aacttctatg ttgtattttt ctattatatc atctggtaag
     2401 tcacatgcac tgtccactat caattttatt ttatccatat taacctcact ataagatatt
     2461 attttattta ttcagtcggt cgtacgaccg atttaattat tattttatat attgaatgaa
     2521 aaaaagtcaa tagatataga ttctcccttt attgacaata aatattcata agcttataat
     2581 gttattaatt tgttataagt atacatggag aggtaggttt tatgaaaaca aataatatga
     2641 aacaggctat tttatcatct gcaactaatt taatagccaa aaatggagtt caaaatacta
     2701 gccttgctga tattgcaaaa gatgtaaata taagtaaagg cactttgtat tatcactacg
     2761 catctaaaaa tgatattata tatgatattg cggatactca tttagaagtt ataactagtg
     2821 ctatactaaa ctgtgtaaaa aatgtcaaat caaaaaattc tcaaatagat atggttaact
     2881 taatcttaga gaaaatctct actatagaaa gtcgtggtag ggtacatatg tatcttattt
     2941 gtgaggcaat tacttcaaat aatgatttaa aagaaagaat gagattaaaa tatattgaat
     3001 ggagaactac tcttaaatct gaaattgttg aatcactaga aaaatataat agtagcgaaa
     3061 atgaacagga tgctgaatca ttttcttttt tactcatgtc aatagttgat ggtttagttg
     3121 tccaaagttt attaaaaaca gaaaaaatac cttatgaaaa tatagcttct tttttaatta
     3181 atcattggat ttaaatttaa tacttagata attaataaaa gtgaactatt taagttatct
     3241 actctaacag ttcactttct tatttattat caataaattt tacttaaaac cttcaatttt
     3301 tatttaaacc ttcatggaat cacaaaataa aaatctctta taaatttaaa atgtttttat
     3361 ttatgttcat gtgtatgctc atctgaatgt tcatgaccat gtgtatgtgt atgttcatgg
     3421 ctatgctcat gagtacctga aaaaccatca tcatgattat ggtcgtgacc tacaccattt
     3481 atatgctcat gttcatgacc atgactgtga gaatgttgat gtgaatgttc attgttatct
     3541 cctccacagc atccttttcc tccagtccca caacattcac ttgtatgtgg attatgatga
     3601 tttcttacta caaatctctc tcttaacata attcttatac ctcctagtat attattgagt
     3661 acttcatgta ctccattaat tacagtatac atctaactag gtataatctc aatacatatg
     3721 gtatttttta acatctatag tattaaagaa acttatcctt aaaacctcca ccttttactt
     3781 cttcaacatt attgacagaa ataaaagctt cactatccat atcataaatt aaatctttaa
     3841 atttacctat ttcatttgca gatattatgc aataaatcat ctttttctta ttctgtgtat
     3901 atgcaccttc tgcctctaaa aaagtgactc ctcttcccat tttattcatt attactttag
     3961 atatttcatc atatttattt gaaattaaaa gtatggattt ttttctatca aagcaatctt
     4021 ttcctatatc catagtaaaa gaaattatat acattgtaat taaagtgtac atcaccaatt
     4081 tagctccaaa taagaaagcc cctgcacata ctacaaaaaa attaataaat aaaaatgtat
     4141 ttttcatagg tatatcatac tttcttttca ttatagctgc tactatatca agtcctccag
     4201 cagtagctct tgctttaaaa gtcaatccca tgcctatacc agttaatgca ccaccataaa
     4261 tacactgtag catagtatca tctatcggga aatatttagc aatatcttgt gttaacccaa
     4321 gtgctaaaga aaatagtaac atattgacaa aacttatcaa taaaaactta ttatcaaaat
     4381 actttctagc tataataaat attggtatgt tcatcaagaa acttgatatt ccctgattta
     4441 tgccaaacaa tttatataat ataacacaaa ttccagctac tcctccactt aataatttgg
     4501 ctggtgctag atataaattt attcctaagg ttgatagaat catacccaat ataataagac
     4561 aatattgtaa aaatatgtta ttatcaatac ttagtttttt gctttcacca tacatcaaaa
     4621 cgcacccctt tgcataataa ataaactacc ctgcaaaaca cacgataaat ttataaataa
     4681 atttatcaga agtagcttac taaaaatatt attcatcctc acttaaacgt ttattcatta
     4741 atattctatc acatgatatg aataatttat atgatttata tagaattatt tattgtaaat
     4801 tttggaatat taaaacattt tagtgaatag ataattatat tacacaaaaa atccctatag
     4861 aaatctataa actaatttct atagggattt tgattaacta actatttttt tacaactttt
     4921 atacctgtca tatggtcgtt taagttttgt tcttctaaag atttatcatc tactctgttt
     4981 atttctaaag ctaaagtttc agacttgatg tattcattaa aatggtcaac agccttagca
     5041 atttcgtcat caccacagta ttctattact atattatcta atacttcaaa gttattagat
     5101 tttctcaatt gctgtacttt agatataaat tctctagcat aaccttcatt aatcaactct
     5161 tcagttagct tagtatctag tataacaaaa agattgtttt caacagatac attaaatcct
     5221 tccttagcag ttatacctat taatactaaa tccttattaa attcaaaagc ttcaccatct
     5281 aaatcaactg ttagagtctc acctttttca agtttattag cagtttcatg agcatttaat
     5341 ttacttaaag ctccaacgaa taaattcatc ttagaaccta ataatggtcc tgcttcctta
     5401 aagtttggtt ttaaactaaa gttcatatac tcatctaaat ccttagcaaa gattacctct
     5461 ttaacattaa gctcttcttt tattaaatca actacatcgc ttattgtatc ttcaaactta
     5521 ccatctacta aaactttttg taatggttga cgaactttta ttctttcaac ttctcttgaa
     5581 gctctaccta aagtaactaa gtttttagct aaatccattt tttcttctaa tttagtatcc
     5641 actaactctt cattacactt aggatattca gctaaatgaa cagatacttc tcctgttaag
     5701 tttctataca tttcctctga catatatggt gcaaatggag ctatagctct acaaagacca
     5761 tgtaacaatt cataagttgt gttgtacact gctttcttat cttctgttaa ttcagttgcc
     5821 cagaatcttc ttcttgaacg tcttatatac cagttagata aatcatcatt tataaaatct
     5881 tgtatcattc ttaaagtttt atttaactca aatatagcta aattttcttc tacatctttc
     5941 attaaattat tgaatttaga taatatccat ctatcaagtt ctggtctatc tttatattct
     6001 acaaaaaatt cagttggatt tatatcatct gtatttgcat ataaagtaaa gaaattatat
     6061 acattcttca tagtacctat aaatttactt tggatttctt ttaatccatc catatcaaat
     6121 ttagttggag tccatggtgg agaaacatat agtaaatacc atcttaatgc atctgctcca
     6181 tattggtcaa ataattccat tggatttact gtgtttcctc ttgatttact cattttctta
     6241 ccatctttat caagtacaag gtctggaact aaaacactct tatatggtgc tttccctaat
     6301 acaaatgttg atactgcaag taatgagtag aaccatcctc tagtctggtc tataccttct
     6361 gatatataat cagctgggaa tagttggtca aagttttcct tattttcaaa tggatagtgg
     6421 tgttgagcaa atggcatagc cccactatca aaccaacagt ctataacttc tgttactctt
     6481 gtcattggtt ttccacattt ttcacatttt aagtgtatat catctacata tggtctatga
     6541 agttcaactg tttctggatt tacatcttct atagctcttt cagctaattc agctcttgaa
     6601 ccaactgaat ctgtatgacc acattcacat ctccatatag gaagtggagt tccccaatat
     6661 ctagttcttg atattgccca gtcatttaga ttttctagcc agtttccaaa tctcttctct
     6721 cctacaaaac ttgggaacca ttttacacca ttattatttt ctattaattt gtctttaagt
     6781 ttagtcatct ctatatacca gcttggtttt gcatagtata ctaatggagt ttgacatctc
     6841 cagcagtgtg gataattatg aactactttt tctttgctat ataatttatt ttcagaagct
     6901 agccatttta ttatttcaac atctagccca tcttccatta caaatctacc ttcccaagga
     6961 gtagatgtaa atttaccagt ttcatctact ggttgaataa ctggtaaatc atatctttta
     7021 cctgtgttgt agtcatcttc accaaatgct ggtgctgtat gaactatacc agtaccatct
     7081 tcagtagtta cataatctcc tactgtaata aaaaatgctt ttttgtcaac ttctacaaaa
     7141 ggcattaatt gttcatattc caaaccttct aggtctttac ctttcatttc ttctaaaact
     7201 tcataatcct cacctagtac tttatcagct aaaggtttag atacataata tatctcatca
     7261 ccttttttaa cttttaaata atctacatca gcatttactg ttaacgaaac atttgatggt
     7321 aaagtccatg gagttgttgt ccatgctaag aaatattcat cagcatcttt tttcttaaat
     7381 ttagctataa ctgtattagt ttttacttct ttatatcctt gtgcaacctc atgactagca
     7441 agaccagttc cacatcttgg acaataagga agtattttgt gaccttcata gatatatcct
     7501 tctttgttga atttattcag tatccaccat acagactcta tatagttatt atctaaagtt
     7561 atatatggat tgtctaaatc aacttcataa gccattctct cagtcattat tctccattgt
     7621 ttttcaaatg agaaaactga ttctctacat ttttggttaa attcagctat tccatactcc
     7681 tctatttgtt gtttactagt aagacctaat tctttttcta cttgtatttc aactggtaat
     7741 ccatgtgtat cccaaccagc ttttcttttt acttgataac cagacattgt tttatatcta
     7801 caaactgaat ctttaagtgt tctagataaa acatggtgta ctccaggatt tccattagct
     7861 gttggaggtc cctcataaaa tacgaaactt gggtcatctt tccctttttc taaagttttt
     7921 tcaagtatat ttatatcttt ccaataatca aaaacttgtg cttctgcttg ttttacagaa
     7981 ctgtctacca atggcttaaa tttcgccatg taaatcaccc tttcctataa atattataat
     8041 tccaaaattt tttattcttt cacataaatt taaatactaa aaaactcgcc ccctttaaac
     8101 caaagggacg agttataaat cgcgttacca ccctaattct atatgtaaac taaatactag
     8161 ctacatataa cactctaata gtataacggt actaaccggc acagcttagt agaatttaat
     8221 cgttcagcct gcagctcaag agtgattttt acctttataa attcattggt tctcagcata
     8281 cccaattctc tgtaaaatta tctaaagata aacgtctcta tcaacgcttt taaaaattta
     8341 tctgtgttta tatgaaattc tataacaaat aatttgaata gtcaatatat ttttgaaaaa
     8401 tataataatt tctatttttc ttacattttt atcctatttt taatttttgt tatagatttc
     8461 tcaaacttac acaatgattc tacataacaa ataaccaaat tacaagctta ttctaaagtt
     8521 gtagcagctt catcattact ataattatac actgttgttc cttcaaatgc atttacacat
     8581 ttttcatcta catcataaaa aatctcatct acacttctta tttcatcttc taacaatgat
     8641 ttgaatttat ttctaaatat cttaaattct ttgactaaag catcatactc ttttcttatc
     8701 tctataacac ggttattagc ttgttctata atttgtctag cttttaaatc agcttcttca
     8761 acaattatct tagccttttt attagccgcg ctacaagtgt cttctgctgc actttgagca
     8821 gttattaaag tagcttttag tgtttcttca atattttcat acttatttac ttggtcttga
     8881 tataacccta atttttctct aagagctgta ttttccttac atagagactc ataatcttcc
     8941 ttgatgatat ctaaaaattc atccacctct tcatctctat atcctcttaa acctttttta
     9001 aactctttat tttctatctc aattggagtt agcattacat ttcctcccag ctataattta
     9061 aattagtcct tatacaatta atcttgcttg aacttttatt tttccttttc tagtaatatc
     9121 tcctatttgt gttaccttag ctcttccttt acctctaata gaaatcaaat catcattttt
     9181 tactatttta gaagttgaag ttatcttttc ataattgaca tggacttttt ctccatttat
     9241 gtattttgca ctatcttgtc tagatatatt gtaaatacca cttatcacac aatctagcct
     9301 atctgaagat actgtaaatg acacttcttt atattttgaa gaactattaa caatattttc
     9361 tctggaaatt tcctttacaa ttacattatt tcttgaaact ttttctaagt ttacaattat
     9421 aaaatcgcaa atatcactac ttacaactac ttgacaaaag ctatcatgta taattatatc
     9481 tccaattttt tctcttttta tacctagacc tagtattgac cctaagtaat ctttatgaga
     9541 tatattttta aatttaaaat tcccttctat ttgtaaaaat ctaagagtat cttctatatc
     9601 ttcatattcc atgtaaaatg gataaataaa tacagtgctt ctttcagctt gttcataacc
     9661 tccatccaca ctgtatttta tttcatttgt agaatttaat attgctacag catttttcac
     9721 ttcatatgga ttaagaaact ccgtagactt aacatcatag ttttttatac aactattagc
     9781 cttgtctatc actttaaaca ttttattttt caagtctata tcctttatgt gatttgttag
     9841 ttttaattta tccatcctat caccttagaa tataaccaat actaattgtc ttaggaacat
     9901 caataatact attgcaatta aaggagaaaa atctataggc ccatttgtat atttatacat
     9961 tatggacctt attggccctt ctattggctc tgtaagattt tctaaaatat caaaaatttt
    10021 actttgtctt ccatttggaa accaagtcat caagcttttt ataactatca tccatgctat
    10081 tatatctaaa agataataaa gtgcaattct tattgttccc atacattaac cgccttttta
    10141 ctatttattt tgccaaggga agaatgcttt actttctaat tcttttttca tatttgcatc
    10201 tatatccaca ttattaggag ctagtataaa tattgattta gaaaccttct gtatacttcc
    10261 atctaataca taaactgccc cattcataaa gttaaatata gatttacgaa cttcagaatt
    10321 agtcaatcct tccaaattca ctataactgc ccttctttgt tttagatggt ctgctatagt
    10381 tgcagcttca tcataaacct taggttcaac tattacaact ttcatttgac tagttgtatg
    10441 taaatttaca attttatttg cttttgcagt actaaatcca ctgttcattt cttcttcttg
    10501 aactatatcg tccataccac tttcatattc atcttcaatg taatcttctt cttcatcaac
    10561 tatccaattc ttaaatttag atattattcc atttgacatt attaacttcc tccttaaaat
    10621 ttattcaatt tgagtaatta ttttttatcg tagtttcttt ctccaaagat tgatgttcca
    10681 actctaacaa tagtggcccc ttcctctata gcaatttcat aatcgtgact cattcccatt
    10741 gataaaacat ccattcctac attagggata tttagttctt ttatttgttt tgataaatct
    10801 ttaagacctt taaatacacc tcttatctca tcttcatctt ctataaaagg tgccattgtc
    10861 attagacctt ttatttttat atttttatat ttttctgaca cttctttgac aaagtctaca
    10921 acttcttctc tatcaagtcc atgcttagat tcttctttag atatattaac ttgtactaag
    10981 caatctattt ctctgtttat ttgctctgca cgtttttgaa tttcttcgca taatgcaatt
    11041 ctatctaaag agtgtatcat acaaacttta tcgattatgt acttaacttt attggtttgc
    11101 aaagttccta tcaaatgcca gtttaatttg ttacctataa tatcatactt tctagctaat
    11161 tcttgtggtt tattttcacc aacagaagta attccttccc tcattgcctc attaactaag
    11221 tccacatcaa cagtctttgt aactgccaaa agggtaatct ccttgctatc tctatctact
    11281 ttttttgcag ctttatcaat tttgcttctt atctcagaca aattgtcttt aatagaaccc
    11341 attattatca cctactttat ccttatattt gtgtttatac tgttaggtct taaaattatt
    11401 ttgtcatata gattaacact tgtaactcca tttaggtcat ttttataata atccacaaat
    11461 acagaagatt cattctcata agttattcct tctaatatga caaattttac actttgtgat
    11521 tgttcattaa ttacatatac accacgttta ttgtcttttg tttttataga agttttaggt
    11581 atttttaaac attccatctg attatatatt atatcaaatt cttcgacact tgtatcctga
    11641 tttgcaacaa tttggttact tatttttaat tttaccatta ttttattatt attttcatat
    11701 attttgtaaa cttttgcatc tattttttgc tcacgatatt caattattac attttgattt
    11761 ttttcaaagt tattagaatc ttctttactg atgtatgtag atagataatt gctttggtca
    11821 ttggcatctt tttctatagc tccttttaca tgaatctttg catcgacttt ttcatttttt
    11881 aacaccaatg tatctacatt tttgctaatt atcattacta atatattaaa tactaaatat
    11941 ataaaaatac caagtatcac taatctggta tatttaacct ttttcaaaga ctctccccct
    12001 ataaaatttc tttcattata agtacttctt ctaaaaaatg tttattcctt ttttataacc
    12061 taatatttta cattctaata attatttttt ccatattaaa tttatttttt atcaatcttc
    12121 ctatacaaga ctgcattatt tatatataat tatattataa agtataagtt taatttacat
    12181 tttatacgtt aagcttactt tatatgtttt atagttattt ttatttttag atgcaatata
    12241 aaaccaatta agatttaaaa taacttttgt tctgttgaca tttatttagg taattattta
    12301 tgcattagga taacgttgtc acaacaaaag tttatgtcct aaaaaaaata caaggaggat
    12361 tttcttatgt cagaaatttt attaggaaca ggtttacttt tattggttct atgttgtttc
    12421 acaattttta gttacaaggc tccatatggt atgaaggcta tgggagcttt agcaaacgct
    12481 gcatgtgcta ctttcttagt agaggcattc aacctttctt tacttggtga tgttctgggt
    12541 atggaatttt ttagaagtgt cggagctgct aatggtagtc ttggtggagt tgctgctgct
    12601 atacttgtgc ctttagcttt aggtgtatct ccagtttatg cagttctaat aggattatct
    12661 tgtttagatt tttctatatt atctggattc gttgctggat actttgtttc tttcttaatt
    12721 aaatttatag agaaaaaagt accacctgga gtggatttag tggttgtaat tcttctatgt
    12781 gcaccactat ctcgtcttat agcatcaaat gtagacccat tagttaatag tacactacta
    12841 caaattggac aagttttgat agaagctaca aacattagcc ctataataat gggaattata
    12901 ctaggaggtc taataacagt ggttggaaca tctccactta gttcaatggc tcttacatct
    12961 ataattggtc ttactggcct tcctatggcg ataggagcat taggtgcttt tggttcagct
    13021 tttatgaact caacgctatt tgcaaaattg aaattaggtt ctaaaaaaga tattatatct
    13081 gttgctattg aaccattgac tcaatcagac ataatttcag ctaatccaat acctatatat
    13141 tctactaatt tctttggagg agccttttct ggtatcattg tagccttgat gggtcttgta
    13201 aacatgactc caggtacagc tacacctgtg gctggttttg ctataatgtt tgcttataat
    13261 ccagttcaac aagttcttat aacagctggt ttatgtgctt tattcaatgt aattgcagga
    13321 tttttaggtt caaaaatatt cagtaccttt aaagttctta ctgctggtga aatacgtggt
    13381 actgatgtag aagaagaaat tgcttaaaaa aacaataaga tttataaatt tatatctcaa
    13441 gtagtagagt tttatattgt aggcaatttt atcaattgca aacgttttat tttttattta
    13501 tgtttatgat tattatgtgt gttgaatatt tagataaatt tttatttatt atttttgttt
    13561 agttttttat ttg
//
LOCUS       DAFNNB010000105        13465 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0106, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000105 DAFNNB010000000
VERSION     DAFNNB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13465
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0106"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            146..2647
                     /locus_tag="L3M60_002511"
     CDS             146..2647
                     /locus_tag="L3M60_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HBN6227166.1"
                     /translation="MSSNKVVESYKITGMTCAACAKAVERVTKKMDGVYDQSVNIATE
                     KLKIEYDNSKVNFDDIKQVVEKAGYGIIKEESNKKIDMKIDGMTCAACAKAVERVVKK
                     LDGVESISVNIATDKANIDYNPSKVKLSQIKAAIEKAGYKPIEEVRNKVDVDEDKLRK
                     EREMKSLFVKFIVAIVFAVPLFYIAMGPMIIKPIGPWPLPEIINPMKNTFNYALIQLI
                     LVIPVMIAGYKFYINGFKALFSLSPNMDSLVAIGTLAAFLYSLYTTIQIANGQIQGMH
                     HHQLYYESAGIIIALILLGKYLESKSKGKTSEAIKKLMGLQPKTAVVLVDGKEVETPI
                     EEVEIGDILLVKPGTKIPVDGVVIEGYTSVDESMLTGESIPVEKNVGSKVTGASINKN
                     GVIKFKAEKIGGDTALAQIIKLVEDAQGTKAPIAKLADTVSGYFVPIVIAIAVVASLL
                     WFLIGGKDIVFVLTIFISVLVIACPCALGLATPTAIMVGTGKGAENGILIKGGEALES
                     AHKVNTVIFDKTGTITEGKPKVTDIVLNNNVKEEYLIKIASSAEKGSEHPLGEAIVKY
                     GEEKNIKFEKVDNFKAIPGAGIQVTINNENILLGNRKLMNDNNIKLGDLEEKSNLLAS
                     QGKTPMYIAVDGNLSGIIAVADVVKESSKKAIEILHDMGIKVAMVTGDNVKTANAIAN
                     QVGIDMVLAEVLPEDKSKEVEKLQNQGKFVAMVGDGINDAPALAKADIGIAIGSGTDV
                     AIESADIVLMKSDLIDVPTAIKLSHETIKNIKQNLFWAFGYNTIGIPVAAGILYVFGG
                     PLLNPMIAAAAMSLSSVSVVSNALRLKNFKAYKRD"
     gene            2807..3475
                     /locus_tag="L3M60_002512"
     CDS             2807..3475
                     /locus_tag="L3M60_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227167.1"
                     /translation="MNSSILVIEDDSNIQELISEFLSAEGYQVDTANDGLEGIQKFKQ
                     GSYDLVILDIMMPNLDGYGVCKMIRKSSSVPIIFLTALNDEGDQLKGFDLECDDYITK
                     PFSFNLLIKRVEAILRRSNKTINDKFIVFEKLKLDLNTYIAEIDGEPIELTLKEFNIL
                     KALIEKYPQVITREGLLDSIWGYDYYGDTRIVDAHIKNIRKKISLPYIKTVKGIGYTL
                     EKDI"
     gene            3459..4736
                     /locus_tag="L3M60_002513"
     CDS             3459..4736
                     /locus_tag="L3M60_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HBN6227168.1"
                     /translation="MRRIFDKWEKLSIKYKLFSITTSLLIALALIIYLILYFLLPSYY
                     HEYKIESLQESLKSLVDSSIHFDTYTLEERLYYMAKDQNLAILLKDNQGKIVYGKNEV
                     VILRYSKYMINSLEDEYRTSIPIYTKDAKDGPYTLELVMPLQPIDEANEVIRKLMPYI
                     ISIAILIAIIGAYIYSIVITKPLINIIESEREQEYRRKDFVATISHELKTPITIISGQ
                     IEGMIYSVGKYKDRDTYLKKSYECTQELKDLVNEMIEVSKSEILEKDLKLVSINISEL
                     LNRLVKRQVFLIEEKHMKTILKIEENLEVKADQERITKAINNIINNAIKYSPEESEII
                     IRLYDKNKRISKKNSNQRVVLEIENTGVTIEKRYLEEIFNPFYRIEKSRSRKTGGSGL
                     GLYIVSQIFKSHGFDYSIKNKENSVIFTVEFKN"
     gene            5139..5360
                     /locus_tag="L3M60_002514"
     CDS             5139..5360
                     /locus_tag="L3M60_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227169.1"
                     /translation="MDRLKRDMKNMKEDLKEGVKKTAYVMKNTAEDLKDDAQGAMVNL
                     EMKKDEMMERYEQKKFSKELEKQMKNEMK"
     gene            complement(5648..6412)
                     /locus_tag="L3M60_002515"
     CDS             complement(5648..6412)
                     /locus_tag="L3M60_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBN6227170.1"
                     /translation="MFAEERIQAILNILKKEGRVTVKELSSKFNVTEDCIRKDLKNIE
                     KISSIRRIYGGAVLARETLENQDTKDRKEINIPTKKIIAEKAFNLIEDRETIFLDIST
                     INILLAKLLAESNKKVTLVTNMLDILNVVTSRPNNLNIISTGGLLNLSLDGFVGTPTI
                     EFISKYKFDKTFMGSCGVDVFNSSLTTFEIEDGLTKKAIINSSKKVFIVMEDKKFKFD
                     GNFKFAHLEDISSIITEKTPTPDIVDILSEFNVNVL"
     gene            6685..7359
                     /gene="fsa"
                     /locus_tag="L3M60_002516"
     CDS             6685..7359
                     /gene="fsa"
                     /locus_tag="L3M60_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBN6227171.1"
                     /translation="MQLILDTGNVEEIKELCTCLPIDGVTTNPSIVSKEKKNFKQLIN
                     EIGEIIGEDMPIHAQVLSTKYEEILEEALYISSLRKNIYVKIPVTQDGLRAIKDLHRK
                     GVKITATAIFTAHQGFLAAKAGANYIAPYVNRLDNISGDGVAMVSELIKIIDTYKMDT
                     KVLAASFKNAQQVIELMQHGVHSATVPYDICKSMMNHPLTDWSVDKFIEDWENTFGKG
                     SKTNNI"
     gene            complement(7481..8875)
                     /locus_tag="L3M60_002517"
     CDS             complement(7481..8875)
                     /locus_tag="L3M60_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBN6227172.1"
                     /translation="MGIILKPKQFYITVIATIILYFSLNYNFNIKFSICLSFLTILIW
                     AIDSVEKTVVALFFVILAFIFNIAPIKVILQFLFTENFYIIILAYIITNAVTKTGVAK
                     IISEKLILNTVNTPKKMILLSYILGILLIFFIPQPFPRVILVSAFYKEFLKEQQITED
                     SKSILLFSIFTASTFTSMFFVNGDTLLNYVVLELGNCNINWGQWAFYMSVPTIITCFI
                     TYFLFIFVFKKELHSIKFVSHENLEVNKIQFSYSNFKKELNLLNKEQKYVFLCLGIMF
                     FMFLTQFIHNINTLIIMSICVLLLLLRKIVGFSTLKEINWKVLIFFIAAFSIGGVLKY
                     SGVVDIMGNYLIKFIPNSSEIISILFLITLTIILNICLGSAVTTSSVVIPLLGSLHIL
                     RENSVTLCLFVYIIVSIQYILPFHHATIMVGHGENLYNSKVIFKYGLTLIPLTYFIII
                     CITFPWWRFIGLEI"
     gene            complement(8954..10300)
                     /locus_tag="L3M60_002518"
     CDS             complement(8954..10300)
                     /locus_tag="L3M60_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6227173.1"
                     /translation="MKDLTTGHEGKSIFFFAMPMLIGSLFQQLYNTADSIIVGRFIGK
                     EAMAAVSGANPIMFLLVAALMGVSLGFSILVSQFYGSGDLKKVKATIDTTYILLFIGS
                     ILISILGIVFGGPMLRLMNTPESVFSQSKLYLTIIFGGILFSAGYNSVSAILRGLGDS
                     ITPLYFLIIATILNIVLDLTFIVVLKMGVEGVALATIIAQAVSFIISIIYLNKKHEVL
                     KFKIKGIIYDNKIFKDGLRLGLPSGVQQMLFSIGNMALQFLVNSYGTSAMAAFGAGLR
                     IENFISLPIMNLGSAVSTFVAQNIGAGENERVKKGIRESIKMTLVLAVIVIALILLFR
                     ENLIALFNTDKDVIKIGSSYLFIIGPFFLFIGTSFVLSSAMKGAGDSMFALISSIVSL
                     WLGRLPASYMLSRFFGTNGIWMGIPFGWTLGLIVTVIYYNKGRWKTKAIVNHRINK"
     regulatory      10550..10652
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            10746..12122
                     /locus_tag="L3M60_002519"
     CDS             10746..12122
                     /locus_tag="L3M60_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBN6227174.1"
                     /translation="MQTTNSSTMQKLFPILTNKNVDMKKEVIAGVTTFLTMAYIIAVN
                     PNILSETGMPAGALVTGTCLAAAFGCILMGVVANLPFALASGMGLNAFFAYTVVLQMG
                     VPWEVALTAVFVEGIIFIVLSVSGVREAVVNAIPKNMKLAVTGGIGIFIALIGLVNSG
                     IVIGDQATLIKMGRFTPAVIITCIGLIIIAVLDKKRVKGSILFGIVVSSLLAWAFAFM
                     NPEHAQKLGIYLPGGLFKFESLAPIAGKIDLGYVLHPKNIGGFLVVVCTFLFVDFFDT
                     VGTLVGVCSKANMLDEKGNVPNVGRALLTDAVATTVGAGLGVSTVTTYVESSTGVIAG
                     GRTGWTAITVGILFLAAMFFSPVFIAIPSCATAPALIYVGYLMLGTVKDIEFDNITEG
                     VPAFVTIACMALTYSIGDGLTLGILTYVFVNIFYNIFGAKKAEDKKHVSIVMIVLAIL
                     FIIKLWLL"
     gene            complement(12224..13369)
                     /locus_tag="L3M60_002520"
     CDS             complement(12224..13369)
                     /locus_tag="L3M60_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBN6227175.1"
                     /translation="MNTFEQLKISSTLIDGLKKQDITSPTEVQSLVIGNIIQNKDLLI
                     NSQTGTGKTLAYLLPIFEKIDTSKRETQALILAPTHELVMQITNQVELLAKNAEISVT
                     SLALIGEVNIQKQIKNIKSVKPHIVIGSCGRVLDLIKQKKLKSHNIKTIVLDEVDNLL
                     NGKNITCIEDIIRTTLRDRQIIGCSASLTDSTIKICDKLMKEFEIIKTKEKSQINPNI
                     NHSYLLGEIRDKFTFLRKTLTATNPKRAIVFVNNEKNIEVLVSKLNYHNYKAIGIFGN
                     MAKEDRKNAINKFKLGKAKILITTDLSARGLDIVDVSHVFNLDFPKSKNEYLHRCGRT
                     ARGNRNGNTISIITKKELDIIKDLQKEFNIVITPKTLQNGELVDIIK"
BASE COUNT         5190 a         1622 c         2116 g         4537 t
ORIGIN      
        1 ttataaaaaa aagattgatt taatatacct acagggggta taataaaaat atagatttct
       61 aaataaataa tttatcaata ctacatatca gaataaaacg ttacaaatta gttgaaaata
      121 tgttgaaaga aaggaagatt ttattatgag ttcaaataaa gtggtagaaa gttacaaaat
      181 tacaggtatg acatgtgcag cttgtgcaaa ggcagtagaa agagtaacta agaagatgga
      241 tggagtgtat gaccaaagtg taaatatagc tactgaaaaa cttaaaatag aatatgataa
      301 ttctaaggtt aattttgatg atataaagca agttgtagaa aaagcaggat atggaattat
      361 aaaggaagaa agtaataaaa aaattgatat gaaaattgat ggaatgacat gtgcagcttg
      421 tgcaaaagct gtagagagag tggtaaaaaa attggatgga gtagaaagta ttagtgtgaa
      481 cattgcaaca gataaagcta atatagacta taatccatca aaagtaaagt tatctcaaat
      541 aaaagcagct attgaaaaag ctggatataa accaatagaa gaggtaagaa ataaggttga
      601 tgtggatgaa gataaattga gaaaagaaag ggaaatgaag tctttatttg ttaaatttat
      661 agttgcaata gtatttgcag tacctttgtt ttatatagca atgggaccta tgataataaa
      721 gcctataggg ccatggcctt tgccagaaat tataaatcca atgaaaaata cttttaacta
      781 cgcattaatt cagttaattt tagtaatacc agtaatgata gctggttata aattttatat
      841 aaatggattt aaagcacttt tttctttaag ccctaatatg gattctttag ttgcaatagg
      901 aacattagca gcatttttgt atagtttata cacaacaata caaatagcaa atggtcaaat
      961 tcaaggtatg catcatcatc agttatatta tgaaagtgca ggaataataa tagcacttat
     1021 cttacttggt aaatatttag aatcaaaatc aaaaggaaaa acatcagaag caattaaaaa
     1081 gcttatgggg ctacaaccta aaactgcagt agtgttggtt gatggtaaag aagttgaaac
     1141 tcctatagaa gaggtagaaa taggagatat attattagta aaacctggaa caaaaatacc
     1201 tgtagatgga gttgtaatag aaggatatac atcagttgat gaatcaatgc ttacaggtga
     1261 aagcatacca gtagaaaaga atgttggaag taaagtgaca ggagctagta ttaataaaaa
     1321 tggtgttatt aagtttaagg ctgaaaaaat tggtggagat actgctcttg cccaaataat
     1381 aaagttagtt gaagatgcac aaggtacaaa agcacctatt gctaaacttg cggatactgt
     1441 atctggatat tttgtaccaa tagtaattgc aatagctgta gttgcatcat tattgtggtt
     1501 tttaatagga ggaaaagata tagtatttgt acttactata tttatatcag tacttgtaat
     1561 tgcttgtcct tgtgcattag gattagcaac gccaactgct ataatggttg gaactggaaa
     1621 gggagcagaa aatggaatct tgataaaagg tggagaggca ctagaatctg cacataaagt
     1681 aaatacagta atatttgata aaacaggtac tataacagaa ggaaagccaa aagtaacaga
     1741 tatagtatta aataataatg taaaagaaga gtatcttatc aagattgctt caagtgcaga
     1801 aaaaggttca gaacatcctc ttggagaagc aatagttaag tatggagaag agaagaatat
     1861 aaaatttgaa aaagttgaca attttaaagc tataccaggt gcaggtatac aagttactat
     1921 taataatgaa aatatattac ttggaaatag aaagcttatg aatgacaata atataaagct
     1981 tggggactta gaagaaaaat caaatttatt ggcaagtcaa ggtaaaacac ctatgtatat
     2041 agctgtagat ggaaatttat caggtataat agcagttgca gatgtagtta aagaaagtag
     2101 taaaaaagca atagaaatac ttcatgatat gggaataaaa gttgcaatgg taactggaga
     2161 taatgtgaaa actgcaaatg ctatagcaaa tcaagtaggt atagatatgg ttttagcaga
     2221 ggttttacca gaagataagt caaaagaagt tgaaaaacta caaaatcaag gcaagtttgt
     2281 tgcaatggta ggagatggaa taaatgatgc cccagccctt gcaaaagcgg acatagggat
     2341 agcaatagga agtggaacgg atgtagctat tgagtctgcg gatatagtac ttatgaagag
     2401 tgatttaatt gatgttccaa cagcaattaa acttagtcat gaaactataa aaaatataaa
     2461 acaaaaccta ttttgggcat ttggatataa tacgattggt atacctgtag cagcaggaat
     2521 attgtatgta tttggaggac ctctgttaaa tccaatgatt gcagcagcag ccatgagctt
     2581 aagttcagtt tcagtcgtat caaatgcact tagacttaaa aacttcaagg catacaagag
     2641 agactagatt aacaagttaa aaagaatgta tccaaaagag ctatctttaa attaaaagga
     2701 ttaatttaca aattaagata gctctttttt catgtaaaat tcacaacaat aagataaaat
     2761 ataagtaata tatgaaattt taaataaaat agaaaggatt gatagaatga attcgtcaat
     2821 acttgtaatt gaagatgatt caaatataca agaattaata tcagaatttt taagtgcaga
     2881 aggttatcaa gtggatacag caaatgatgg gttggaaggt atacaaaagt ttaagcaagg
     2941 aagttatgat ttagtaatat tagatataat gatgccaaat ttagatggtt atggagtatg
     3001 taaaatgata aggaagtcat caagcgttcc aattatattt ttaactgctt taaatgatga
     3061 aggagaccaa cttaaagggt ttgacttaga atgcgatgac tatataacaa agccattttc
     3121 atttaatctt cttataaaaa gagtagaggc aattttaagg cgaagtaaca agactataaa
     3181 tgacaaattt atagtttttg aaaaattaaa attagattta aacacatata ttgcagaaat
     3241 agatggagaa cctatagaac ttacactaaa agagtttaat atattaaaag ctttgataga
     3301 aaagtatcca caggttataa ctagagaagg tcttttggat agtatatggg gttatgacta
     3361 ttatggagat actaggattg ttgatgcaca tataaaaaat ataagaaaaa aaatatcatt
     3421 accatatatt aaaactgtaa aaggaatagg atatacactt gagaaggata tttgataagt
     3481 gggaaaagtt aagtataaag tataagttat tttcaataac gacatcatta ttaatagcat
     3541 tagctttaat aatatatcta attttatatt ttttattgcc atcatattat catgagtata
     3601 aaattgagtc tcttcaagaa agtttaaagt ctttagtgga tagttcaatt catttcgata
     3661 catatacttt ggaagaaaga ctttattata tggctaaaga ccaaaatttg gctattttat
     3721 taaaagataa ccaagggaaa attgtatatg gtaagaatga agtagttatt ttaagatata
     3781 gtaagtacat gattaatagt cttgaggatg agtataggac ttctatacct atatatacta
     3841 aagatgcaaa agatgggcca tatactttag agctcgttat gccattacaa ccaattgacg
     3901 aagcaaacga agttataaga aaactgatgc cgtacattat atcaatagca atattgatag
     3961 cgattattgg ggcatatata tattcaattg taataacaaa accgcttata aatataatag
     4021 agagtgagag agagcaggaa tatagaagga aggattttgt cgctacaata tcacatgaac
     4081 taaaaactcc aataaccata ataagtggtc aaattgaagg tatgatttac agtgtaggta
     4141 agtataagga tagggatact tatctaaaaa aatcgtatga atgtacccaa gaattaaaag
     4201 atttagtaaa tgaaatgata gaggtttcaa aatcagaaat acttgaaaaa gatttgaaat
     4261 tggtatcaat aaatataagt gaattattaa atagacttgt aaaaagacaa gtatttttaa
     4321 tagaagaaaa acatatgaaa actattttaa aaatagaaga aaatttagaa gttaaggcag
     4381 accaagagag aataactaag gcaatcaata acataataaa taatgccata aaatattctc
     4441 ctgaagaatc agagattata attagactct atgataaaaa taaacgaata agtaaaaaga
     4501 actctaatca aagagtggtt ttagagattg aaaatactgg tgtaactatc gagaaacgtt
     4561 atttagaaga gatatttaat ccattttata gaatagaaaa atctcgttca agaaaaactg
     4621 gaggaagtgg actgggactt tatatagtca gccaaatttt taaaagtcat gggtttgatt
     4681 atagcataaa aaacaaagaa aactctgtaa tattcacggt cgaatttaaa aattaattac
     4741 ttattataaa gaatatataa ctttcaataa tagttcattt taaaatcaca aaaaattcat
     4801 actgaaactg tattatataa atataaagaa gctttaaacc ataaaataca ccaactataa
     4861 tgatgagatt tccttacatc ctccatagaa gtcatcatta tagtaaccca acaattaagt
     4921 tactatgaat ttaaaaaata acaccagaca tataatctat gtttagtgtt atttttattt
     4981 tgaaataaaa aatttctaat ttattgaaat gtatagattt agatagtctt aaactatatt
     5041 taagggctta aaaattataa ttgatattac aaataaatat atatactttg ggaaaaataa
     5101 atatagaaag tattatattt aaagaggagg tagttttgat ggacagatta aaaagagata
     5161 tgaaaaatat gaaagaagat ttaaaagaag gagttaaaaa gacagcttat gttatgaaaa
     5221 atacagctga agatttgaaa gatgatgcac aaggtgctat ggtaaactta gaaatgaaaa
     5281 aagatgagat gatggaaaga tatgaacaaa agaaattttc taaagaatta gaaaagcaaa
     5341 tgaagaatga aatgaaataa gtttttattt atgaataaca aattattgtg ttcatataaa
     5401 aagtagctag taatattaaa tagtttattt aaggaaatag actttctaaa aataactgta
     5461 ttagatttag aaagtctatt tcaaattagt tttatgaata gacataagga taaaatcgat
     5521 attatagaac taaattttaa aaaatagata caaggataaa ataaatatta tagaaatatg
     5581 tttttaagga tagctatcaa ataaaaattt atgtttataa gaaattctat aaaatatata
     5641 attaaactta taaaacattt acattaaatt ctgataaaat atctactata tcaggagtag
     5701 gcgttttttc tgtaattata gaactaatat cttctagatg agcaaacttg aaatttccat
     5761 caaatttaaa ttttttatct tccataacta taaatacttt tttacttgaa tttattatag
     5821 ccttctttgt aagaccatct tcaatttcaa aagttgtaag tgaagagttg aatacatcta
     5881 ctccgcaact tcccataaaa gtcttatcaa acttatattt ggatataaac tctatagttg
     5941 gagtaccgac aaacccatct aaactgagat ttaataaacc tccagtggaa attatattta
     6001 aattgtttgg tcttgaagta actacattta gtatatccaa catatttgta acaagtgtta
     6061 cctttttatt actttcagct aacagttttg caagcaatat gttaattgta gagatatcta
     6121 aaaatatagt ttctctatct tcaattaaat taaatgcttt ttctgctatt attttctttg
     6181 ttgggatatt aatttctttt ctgtcttttg tatcctgatt ctctagtgtt tctctagcta
     6241 gtacagctcc tccgtaaatt cttcttattg aagaaatctt ttctatattt tttaaatctt
     6301 ttcttataca atcttctgta acattaaatt tagaactgag ctctttgaca gtaactctac
     6361 cttctttttt taggatattt aatatggctt gaattctttc ttctgcaaac attttctacc
     6421 tcttttccaa atatagtatt atagtaaata ttataagata aaatacaata tatcaactat
     6481 tttaacatga aatacaaagt taatttaata taaaaagcat attttttcta actatctatt
     6541 tacgatttag taaataaaga aattataaaa ttaagaagaa aaataaaaca ataaaaaaaa
     6601 ataaaaaaca ataagcaaaa tgttgaaaat aatgccaaac caatatataa taagaataaa
     6661 caaagattaa agggggatat tttaatgcaa ctaatcttag atacaggtaa tgtggaggag
     6721 attaaggagt tatgtacttg cttaccaata gatggtgtaa ctacaaatcc aagtattgtt
     6781 tcaaaagaaa aaaagaattt taagcaactt ataaatgaaa ttggtgagat tataggtgag
     6841 gatatgccta tacatgctca ggtattaagt acaaagtatg aagaaatatt agaagaagca
     6901 ttatatataa gtagtttgag aaaaaatata tatgtaaaaa tacctgtaac acaagatggt
     6961 ttaagagcta taaaagacct tcacagaaaa ggtgtaaaaa taactgctac tgcaatattt
     7021 actgctcatc aaggcttttt agctgcaaag gctggagcaa attatattgc accttatgta
     7081 aataggcttg ataatatatc tggtgatgga gtagctatgg tttcagaact tataaaaata
     7141 atagatactt ataaaatgga tacaaaagta cttgcagcca gttttaaaaa tgcacaacaa
     7201 gttatagaat taatgcaaca tggggtgcat agtgctacag taccatatga tatttgtaaa
     7261 tctatgatga atcatccatt aactgattgg agtgtagata agtttataga ggattgggag
     7321 aatacttttg gaaaaggaag taaaacaaat aatatttagt attatggtaa aaaagcagtt
     7381 ttgttcaatt tcacaataac ttgtataaaa agacgaaaat atgaattttc tttttaataa
     7441 ttccgttatt atcttaaatc aattcatatt ttcgtctttt ttaaatttct aatcctataa
     7501 atctccacca agggaatgtt atacatatta ttataaagta ggttaaagga atcaaagtta
     7561 aaccatactt gaaaataacc ttgctattat acagattttc accatgaccc accattattg
     7621 tagcatgatg aaatggtaat atgtattgta tacttactat aatgtagaca aatagacata
     7681 aagtaacact attttccctt agtatatgca aacttccaag cagtggtatt actacagatg
     7741 atgtagtaac agcacttcca agacatatat tcaagattat tgtgagagtt atcaaaaata
     7801 atatcgaaat aatttctgaa gagtttggta taaattttat caaataattt cccattatat
     7861 ctacaactcc ggaatacttt aacactcctc caattgaaaa tgctgctata aaaaaaataa
     7921 ggaccttcca atttatttct tttaaggtgc taaatccaac tatttttctt agtaataaaa
     7981 gaagtacaca aattgacatt ataattaatg tatttatgtt atgtatgaac tgagttaaaa
     8041 acataaaaaa cattattcct aagcataaga atacgtattt ttgttctttg tttagcaaat
     8101 taagctcttt tttaaaatta gaatatgaaa actgaatctt attaacctca agattttcat
     8161 gagaaacaaa ttttattgaa tgtaattctt ttttaaagac aaagataaat aaaaaataag
     8221 ttataaaaca agtaattata gttggaacag acatataaaa agcccattgc ccccaattta
     8281 tattacaatt tccgagttct agtacaacat agtttaggag agtgtctcca tttacaaaaa
     8341 acattgatgt aaaggtagag gctgtaaata tactaaataa aagtatagat tttgaatcct
     8401 cagttatctg ttgttctttt aaaaactcct tgtaaaaagc agatacaaga ataaccctcg
     8461 gaaatggttg tgggataaaa aatattaaaa gtatacctaa aatatatgat aaaagaatca
     8521 tttttttagg ggtatttaca gtatttaaaa ttaacttctc agaaataatt tttgcgacac
     8581 ctgtttttgt aactgcatta gtaattatgt atgcaagaat tattatgtaa aaattttctg
     8641 taaataaaaa ctgtaaaata acctttattg gtgcaatatt aaagataaat gctagaatta
     8701 caaaaaacaa tgcaacaaca gttttttcaa ctgagtctat agcccatatc aagatagtta
     8761 aaaaagatag gcatatagaa aattttatgt tgaaattgta attaagagag aagtagagaa
     8821 ttatagtagc tataacagtt atataaaatt gttttggctt aagaataata cccattttta
     8881 tattcacctc cataatttat tatagcataa tgaaggtgaa tacaatgaag gtgaatacat
     8941 gtttataaaa agtttattta ttgattctat gatttacaat ggctttagtt ttccaacgcc
     9001 ctttattata gtagataact gtgacaataa gacctaaagt ccaaccaaaa ggaataccca
     9061 tccaaattcc atttgttcca aaaaatctag aaagcatata agaagctgga agtcttccaa
     9121 gccataaaga tactattgag cttataaggg caaacataga gtcaccagca cctttcattg
     9181 cacttgaaag cacaaaagaa gtgccaataa ataaaaagaa tggtcctatt ataaataagt
     9241 aagaagagcc aattttaatg acatctttat cagtattgaa tagagcaatt agattttctc
     9301 taaacagcaa aattaaagca ataactataa ctgctaaaac taaggtcatt tttatagatt
     9361 ctcgaatgcc ttttttgact ctttcatttt ctccagcacc tatgttttga gcaacaaaag
     9421 tagaaacagc agaaccaagg ttcataatag gtaagcttat aaaattttca attctaagac
     9481 cagcaccaaa agccgccata gcagatgtac cataactatt aactaaaaat tgaagagcca
     9541 tatttccaat tgaaaataac atttgttgaa ctccacttgg aagtcctaat ctaagcccat
     9601 ctttaaatat tttattatca tatataattc ctttgatttt aaatttaagc acttcatgtt
     9661 ttttatttaa atagattata ctgattataa atgatactgc ctgagcaatt atagtggcaa
     9721 gtgcaacgcc ctcaacaccc atttttagga ctacaataaa agttaagtct aaaactatat
     9781 ttaggatggt ggctattatt agaaaataaa gtggagttat tgaatctcca agtcctctaa
     9841 gtattgcact tactgaatta tatccagcag aaaataatat tccaccaaat attatagtaa
     9901 gatacaattt tgattgagaa aaaacggatt ctggtgtatt cataagccta agcatgggtc
     9961 caccaaatac tattccaaga atacttatta atatagaacc aataaataaa agtatatatg
    10021 tggtatcaat agtagctttt acttttttta aatctcctga cccataaaat tgtgatacta
    10081 aaatagaaaa tccaaggcta acacccataa gtgctgccac caataaaaac attatagggt
    10141 tagctccaga gactgccgcc attgcctctt ttccaataaa tctaccaact attatactgt
    10201 cagctgtatt gtatagttgt tgaaataaac taccaataag catgggcatg gcaaaaaaga
    10261 atatggattt tccctcatga cctgtagtta aatctttcac cttattcctc caaataaaaa
    10321 aattattaat ttattatagt ataatcttaa cataaacttt atatccaaac tataaataaa
    10381 ttgtatcaaa atatcatata tttttttaaa atgtaaagaa tatagttata atttgaattt
    10441 aataaattaa tatttaatat aatcgaatat taatacaaaa tcaacacaat atatacgtat
    10501 atattgacaa aattaattat ataaattaaa ataatattcg taaagaaaaa atcatgtttt
    10561 atactgtata acatataatt ttgacaatat gggtcataag tttctaccgg aataccgtaa
    10621 atattctgac tatgtatact attatttagt taattaaata tttaattaac tgaattatga
    10681 aggatacggc tcagcttatc aactactgtg tcgttttttt gtaaaattta ataggaggtg
    10741 ttgttatgca aacaacaaac agtagtacta tgcagaaact ttttccaatt ttgactaata
    10801 aaaatgtcga catgaagaag gaagtaattg caggagtaac tactttttta actatggctt
    10861 atataatagc tgtaaatcca aatattttat ctgaaacagg aatgcctgca ggtgcacttg
    10921 ttacaggtac ttgtttagca gcagcatttg gatgtatttt aatgggtgta gttgctaatc
    10981 ttccttttgc cttagcatca ggtatgggtc ttaatgcatt ttttgcatat acagtagttt
    11041 tacaaatggg tgttccttgg gaagtagctc ttacagcagt gtttgtagaa ggtattattt
    11101 ttatagtatt atcagtaagt ggagttcgtg aagctgttgt aaatgcgata ccaaaaaata
    11161 tgaaattagc tgttacaggt ggtataggaa tatttattgc acttataggt ctagttaaca
    11221 gtggaattgt tattggagac caagccacac ttatcaaaat gggtagattt acaccagcag
    11281 taataataac ttgtatagga ttaataatta ttgctgtatt agataaaaaa agagttaaag
    11341 gttctatact ttttggaata gtagtgagtt cattacttgc ttgggctttt gcttttatga
    11401 atccagagca tgcacaaaaa ttaggaatat atttacctgg tggattattt aaattcgaaa
    11461 gtttagcacc aatagcaggt aaaatagatt taggatatgt actacatccg aaaaatatag
    11521 gtggtttttt agtagtagtt tgtacatttt tatttgttga tttctttgat acagtaggta
    11581 cattagttgg tgtttgttct aaagcaaata tgttagatga aaaagggaat gttcctaatg
    11641 taggtagagc tttacttaca gatgcagttg caactacagt tggtgcagga cttggagtat
    11701 ccacagttac aacttatgtt gagagttcaa caggagttat tgcaggtgga agaactggat
    11761 ggacagcaat aacagttgga attttattct tagctgcaat gtttttctct cctgtattta
    11821 tagcaattcc atcatgtgct acagcaccag ctttaatata tgttggatac ttaatgcttg
    11881 gtacagttaa agatatagag tttgataata taactgaagg tgttccagct tttgttacta
    11941 ttgcttgtat ggcattaaca tatagtatag gtgatggttt aactttaggt atattaactt
    12001 atgttttcgt aaatatattt tataatatat ttggagctaa aaaggcagaa gacaaaaaac
    12061 atgtttctat agtaatgatt gttctggcaa tattatttat aataaaactt tggctattat
    12121 aatagatatt atttaaaatt ttaatttaat ataaaataaa tagctgtaat ttctcatcaa
    12181 ttttgatgga aaattacagc tatttcgtat ttatttcatt ttattattta ataatatcta
    12241 ctagctctcc attttgtaaa gttttaggtg taattactat attgaactct ttttgcaaat
    12301 cttttataat atccaattct ttttttgtta ttattgagat tgtgttacca tttctatttc
    12361 ctctagcagt tcgaccacat ctatgcagat attcgttttt actttttgga aaatctaagt
    12421 taaaaacatg acttacatcc actatgtcta gtccacgagc agataaatca gttgtaatta
    12481 aaatctttgc tttaccaagt ttaaatttat ttattgcatt ctttctatct tcttttgcca
    12541 tatttccaaa aattcctatt gctttgtaat tatgataatt taattttgat actagaactt
    12601 ctatattttt ttcattattt acaaatacga tagctctctt tggatttgtg gctgttaatg
    12661 tttttctaag aaaagtgaac ttatctctta tttcacctaa caaatatgag tgattgatgt
    12721 ttggatttat ttgtgatttt tcctttgttt ttattatttc aaactctttc ataagtttat
    12781 cacaaatttt tatagtgctg tcagtaagac ttgcagagca tcctataatt tgtctatctc
    12841 ttaatgttgt tcttattatg tcttctatac aagttatatt ttttccattc aatagattgt
    12901 ccacttcatc taagacaata gtttttatat tgtgtgattt taactttttt tgttttatca
    12961 agtctaaaac tcttccacaa gagccaatta ctatgtgtgg ttttactgat tttatatttt
    13021 ttatttgttt ttgtatattt acttctccaa ttaaagctag agaagtaaca gagatttccg
    13081 catttttagc caataattca acttgatttg taatctgcat tactaattca tgcgtaggtg
    13141 caagtataag agcttgtgtt tctctttttg atgtatcaat tttttcaaat ataggtaaaa
    13201 ggtatgctag tgtttttcct gttccagtct gtgaattaat caataaatcc ttattttgaa
    13261 ttatattccc tataacaagt gactgaactt ctgttggaga agttatgtct tgttttttaa
    13321 gtccatctat tagagtggaa cttattttta gttgttcaaa tgtattcata ttttgctcct
    13381 tatatttaat aattatagtc aactattttt tattatacat tgtttaggca aatcgaacta
    13441 tatattcttt tataattatg tgtaa
//
LOCUS       DAFNNB010000106        13463 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0107, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000106 DAFNNB010000000
VERSION     DAFNNB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13463
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0107"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            240..1178
                     /locus_tag="L3M60_002521"
     CDS             240..1178
                     /locus_tag="L3M60_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6227176.1"
                     /translation="MFRKVLYSLTLLSISIIFLVGCSNSQNKQNANENKEIQSQEDTK
                     KIDSSKDTSNVIVSDGIDKPSKNATNNDNNKLDVSSLDNTALDWFYIPNNKHKTPEVN
                     TDIGFKFSDYDAIYNGPTKDGQKTLYLTFDEGYENGYTTKILDTLKQNQVKAVFFVTY
                     PYIKENKDLVKRMVSEGHIVGNHSKTHPSMPTKTSNLKNFNDELYDVEKLYKDVTGKN
                     MVKFFRPPMGKYSEKSLAMTKNLRYKTVFWSFAYRDWDTKKQPPHEEATQKIMANLHD
                     GSILLLHAVSKTSTEILNDFITNARKLGYEFELLEY"
     gene            complement(1535..2908)
                     /locus_tag="L3M60_002522"
     CDS             complement(1535..2908)
                     /locus_tag="L3M60_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227177.1"
                     /translation="MVLTGAHYIYILFMVIILITMIMKKDTIVPCILGVFFMGLFFEK
                     NIFGAITAVFNSFIISLNELGPIILIIAIMVALSKALEANNAIQYMVRPFSRVIKNSN
                     TAFFVTGFVMLVLSWFFWPTPAVALVGAVFLPVAMRAGLPAIGVAVALNLFGHGLALS
                     TDFVIQGAPSITAGAAGVAVSYVINDGMILFWVMGIVTISVAFYTLKRDINKGMFREE
                     LKGFESEEVEEFNGKSKIATILVALGFLADIIAMYMFDLKGGDASALLGGTAVFLIII
                     INVINFGKDSLENVCENIIDGFVFGIKIFGAIIPIAAFFYMGEVAPLTGVFGKVLAPG
                     SQGLLSDIGIALSQTIPLNKFAVSGIETVVGAITGLDGSGFSGISLVGSLASVFGTAI
                     NASVGALAALGQISGIWVGGGCLVPWGLISAAAICGVSPIELAKRNFIPVITGLIVTT
                     IVAMFII"
     gene            complement(3074..4396)
                     /locus_tag="L3M60_002523"
     CDS             complement(3074..4396)
                     /locus_tag="L3M60_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227178.1"
                     /translation="MISKIKPSKLGVILLMYVVISAAVAFILNIPFYSAVESIVKDVS
                     EGKVYFKREVNRLMDDFQTYVNNNGIRADDGDDIAKWNSDNWFVSIKIYKGNRVFYDT
                     LYYPNEVPDKSEENEFYSSPMARNYEIQFSDIKANIIVVALFRFRFQNFIEAISITFM
                     ALTFIITFLLLFSRKIKYLRKIEMGIKIVESGSLKYRIPVKGNDELSSLASSINEMSN
                     TLEKQLIKEREMKNKNKDIVTSISHDVRTPLTSVICYLDLIKDHKYNSPEQLEQYIKN
                     VRSKAYQIKDLTDDLFTHFVNSGEEVSKGYEKINANEFIFQLLLDSSYTLEEKGFHIT
                     IENELYNQYHIYVDVTQFRRIFDNLCSNIIKYARKDKPIVFLIKVEDSKLLIIQKNRV
                     SNFSKNVESFGIGIKNCEKIVAYHNGSINVNSDDKEFAIEISIPAKES"
     gene            complement(4389..5093)
                     /locus_tag="L3M60_002524"
     CDS             complement(4389..5093)
                     /locus_tag="L3M60_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227179.1"
                     /translation="MNKEIPKKILVADDNPEIREIVEILLTGEGYEVIMATNGEEAVN
                     LADSTIDLIILDVVMPVKTGFVACSEIREKTTAPILFLTAKTQDSDKVIGFSAGGDDY
                     LSKPFSYSELISRVKSLLRRYYIYQGKEKEKEPNILKTKELCVNLDTQDVTLNGQPVF
                     LTTIEYSILVLMLKNRKKVFSSENLYETIWDEQYFYTANNTIMVHIRNLRKKLEKDQK
                     NPQYIKTAWGKGYYID"
     gene            complement(5276..6340)
                     /locus_tag="L3M60_002525"
     CDS             complement(5276..6340)
                     /locus_tag="L3M60_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBN6227180.1"
                     /translation="MISKTEKKNKCLGSVAFLVLLMGITGYFVFRGQSVESLIKSLKG
                     ASPMFILIGFIMMFIYVACEGINIYLGMKALRQKTTLLKCMGYAFIGFYFSSITPSAS
                     GGQPAQVYYMKKDDINISYSSLILLVIVVIHQVVILAYSGVMFIMEREFILNNVSGMN
                     ILLIYGVITNVALVVGVIAIIFSKKLVNNFIISITNLLGKLRIIKDVESSRKVITSQI
                     EEYVKGAQYIKQNPKLVVQILVITIIQITAMFLVPFFVYKAFHLSTYTVFEILAIQSL
                     LNIAVSSLPLPGAVGASENSFMTLFKIFFPGHLLVPAMLLSRGISFYAFVAISGLICI
                     IVHVKSSEKVKSVKKVAYVR"
     gene            complement(6343..7041)
                     /locus_tag="L3M60_002526"
     CDS             complement(6343..7041)
                     /locus_tag="L3M60_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6227181.1"
                     /translation="MYVVGLIIIVALIFLVHSIIPTYYNKLLNKEVLKNMAGENEIAL
                     TFDDGPDKRYTEKLLDVLKENDIQAMFFVVAKNAEKEPEIIKRMLRENHIIGLHSLEH
                     KNAWLYSYSYVKKDFIESTNIMKNLGVDVSYYRPPWGHTNIFSNLFVKKYNLKMTLWD
                     VMAEDWNKNSTVDIIINKLMSRTKENSIICLHDAGENSGGAVGAPERTIEALKVAIPK
                     LKASGLKFVTPERM"
     gene            complement(7032..8129)
                     /locus_tag="L3M60_002527"
     CDS             complement(7032..8129)
                     /locus_tag="L3M60_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-diphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBN6227182.1"
                     /translation="MKVLILTGKFGMGHYSASNSLSEDIKAKFDNSEITIKDIFEYIM
                     PNYSDKMYKTFSILVNRGSSLYNLFYKCAENGKKDIKFTFSDYFLNKLDELLNEVQPT
                     VVISTFPFCSQLVSRYKEKYNSNLPLITCITDISSHSEWISKNTDCYLVASKSTKEEL
                     VFKGIDESKIKVNGIPVKKEFKRIEHINHSTKKNILIMGGGLGLLPKSEQFYRELNDL
                     EGVKTTVITGNNKKMYYKLYGRYENIEVVGYTNEVYKYMKGADLIISKPGGITLFETI
                     YSELPILAFNPFLQQEIDNASFILNNEIGRILGKNKKYYVDEIKDLIYDDVTLKEMSS
                     NMKELKKQFDNNTLENILFSLDEQGECRECM"
     gene            complement(8454..9698)
                     /locus_tag="L3M60_002528"
     CDS             complement(8454..9698)
                     /locus_tag="L3M60_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBN6227183.1"
                     /translation="MFENLKKADPEIYESMKRELKRQQRNIELIASENIVSVPVMETM
                     GSHLTNKYAEGYSGKRYYGGCEYIDEVETLAIDRIKKIFGAEHANVQPHSGANANIGV
                     YFAMLKPGDVVMGMNLSQGGHLTHGAPVNISGQYYKFYEYGIDKDSGLIDFDEVRKIA
                     HEVKPKMIVAGASAYPREIDFKKFREIADEVGALLMVDMAHIAGLVAAGLHQNPCEVA
                     DFVTTTTHKTLRGPRGGVILCKEKYAKDIDKAIFPGIQGGPLEHIIASKAVCFKEALS
                     DEFKEYQVQVAKNAKALAEELIKRDFKLISGGTDNHLILLDLTNKNITGKAAEKRLDD
                     AYITANKNTIPFDPNGALVTSGIRLGTPAVTTRGMKEEDMAIIAEAIDLCLTYDEESK
                     ARTLVVGLTEKYPLYEEYNIMD"
     gene            10026..10814
                     /locus_tag="L3M60_002529"
     CDS             10026..10814
                     /locus_tag="L3M60_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBN6227184.1"
                     /translation="MNQNEELEYKKNILKLALFVGELMIKNGAETSRVEDSVLRICRS
                     RGFYHVNIFTTPTVIIISDEKFDGFSFMKTIQSRGINLNKISLLNSFSREFVSDKEYD
                     IDNAIARLYEIQDVKPYPLWLFYSCTGIASACFACLLGGNYVLNFILTFIIAIFAAIT
                     YDKTMKISAISAFSCLVSSFLIALSGVLLVEIGILDDPKMLIVGAIMPLLPGVAFIKA
                     IRDLISGNLISGISRAFDAAMIIIAIASGVGFVLDTWYRIGGPF"
     gene            10814..11287
                     /locus_tag="L3M60_002530"
     CDS             10814..11287
                     /locus_tag="L3M60_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBN6227185.1"
                     /translation="MTDMSMILHFLFSGVATAGFAVFFNAPLYLLLPAGITGGIGWIV
                     YVYLFNFTTNAVFAGFIAAALVSACSETLARKLKQPAIVFVIPGILPLIPGIGLYNTM
                     LSLIQKNYSLAMSKGTDALFLSAAIALGVLVVTSFVRTLNLLKIRKNFIPYRKSK"
     gene            complement(11379..11819)
                     /locus_tag="L3M60_002531"
     CDS             complement(11379..11819)
                     /locus_tag="L3M60_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HBN6227186.1"
                     /translation="MNQQGFIMEIVDDRTAKLKMQRHSACAACGKCATTNSAESKEIL
                     VEVDNTIGAKVGDHVEVSMDNMNVLKAAVMAYIIPLIALLVGTIGTYYILGAIGLKTG
                     VEAISGLVGICLTIICYLYLKKNDKKFRDSREFIPVITRILIDL"
     gene            complement(11834..12127)
                     /locus_tag="L3M60_002532"
     CDS             complement(11834..12127)
                     /locus_tag="L3M60_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HBN6227187.1"
                     /translation="MEERKLAPNNEREALIKRLKRIEGQVKGIQKMVEQERYCVDILV
                     QISAIRSAINRVGTIILENHIKGCVAESIKHDDADKTEEMIKELMDTINKFTK"
     gene            complement(12170..12571)
                     /locus_tag="L3M60_002533"
     CDS             complement(12170..12571)
                     /locus_tag="L3M60_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227188.1"
                     /translation="MKKCLYCNRDIKSPNNNLSIKYDDNTNIYPCRAECKEKIEEYIA
                     KKPTYKFINILEVLLGVGGIFCFLSKWTIAAQVVLGIDALVIFLFPLAGFKMNGKLSM
                     EQTKNQSRSIAISILVFIVIITVLYFKQVGK"
     gene            12804..13448
                     /locus_tag="L3M60_002534"
                     /pseudo
     CDS             12804..13448
                     /locus_tag="L3M60_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
BASE COUNT         4639 a         2213 c         1586 g         5025 t
ORIGIN      
        1 actcattcaa tgtctttgtg cttgttattt tgttacttct tgttgagcac atttattata
       61 ataacacctt ctaacaatat ggtcaactac ttttttcatt ttttttaaac tttattgatt
      121 attcacccat tgtcatacta ttgtgacttt atttttaaga atgtaactat tattaataat
      181 agtttacaat tataataata ctatatgtag ttgttattaa ttcagaaagg agcttcaaga
      241 tgtttagaaa ggtattatat tctttaacac tactttctat atctattatt ttccttgttg
      301 gatgttcaaa ttcacaaaat aaacaaaatg caaatgaaaa taaggaaata cagtcacaag
      361 aagatacgaa aaaaatagat agtagtaagg atacttccaa tgtaatagtt tcagatggaa
      421 tagataaacc atcaaaaaat gctactaata atgataataa caaattagat gtatcctctt
      481 tagataacac tgctcttgat tggttttata taccaaacaa taagcataag acaccagaag
      541 taaatacaga tattggattt aaattttctg actatgatgc tatatacaat gggcctacaa
      601 aagatggtca gaaaacacta tatcttacat ttgatgaagg ctatgaaaat ggatacacca
      661 caaagatact cgacaccttg aaacaaaatc aggttaaagc agtttttttc gttacatatc
      721 catatataaa ggaaaacaaa gatttagtaa aaagaatggt ttctgagggg catatagttg
      781 gaaatcacag taaaactcat ccatctatgc caacaaaaac aagcaattta aaaaacttta
      841 atgacgaatt atatgatgtt gaaaagttat ataaagatgt aactggaaaa aatatggtta
      901 aattttttag acctcctatg ggaaaatatt cagaaaaaag tttagcaatg actaaaaatt
      961 taagatacaa gactgtattt tggagttttg catatagaga ctgggatact aaaaaacaac
     1021 cacctcatga agaggcaaca caaaaaataa tggctaatct acatgatggt tctatcttgt
     1081 tactacatgc agtttctaaa actagtacag aaattttaaa tgattttata acaaatgcaa
     1141 gaaaactcgg atatgaattt gaactacttg agtattaatg tttagaatta catctaaaat
     1201 ctacaaacta gtatagttaa tatagtatat gtgataaaat aataatttaa aattagaatt
     1261 tttgtttggt aatagtatag gtaaaaaact tgtgatgtct tacatatgtt ggtagtacaa
     1321 gcaataaaaa ctcaataaat ttggcaagat actaaaatag ataatagtat tttagatgtt
     1381 atattagtat aacaaaaaat attattctat caaagaatac tattaaaaat atcaataaaa
     1441 atagatgtat ggcttagaat tagtaaaact gttatgataa attatagcta cacatctatt
     1501 tttagtttgt ataattaaag ttttatttaa ttcattaaat aataaacatt gctactatag
     1561 tggttacaat caatccagtt ataactggta taaaatttcg ctttgccaat tctattggac
     1621 taactccaca aattgccgct gctgaaataa gcccccaagg aactaaacag cctccaccta
     1681 cccatatacc actgatttgt cctaatgcag ccaatgcacc aacacttgca tttatagcag
     1741 ttccaaacac agatgcaagt gaaccaacta atgaaattcc agaaaatcca gaaccatcaa
     1801 gtccagttat tgctcccact acagtttcta tacctgatac agcaaattta ttaagtggta
     1861 ttgtctgaga aagagctatt cctatatctg ataataaacc ttgtgaacct ggtgctaata
     1921 cctttccaaa tacacctgtc aaaggtgcaa cttcacccat atagaaaaaa gctgctattg
     1981 gtataattgc gccaaatatt tttatcccaa acacaaaacc atcaattata ttctcacata
     2041 cattttcaag actatctttg ccaaaattta taacatttat aattattatt aaaaatactg
     2101 ctgttccacc taaaagagct gatgcatctc ctccttttag gtcaaacata tacattgcaa
     2161 ttatatcagc aagaaatcct aaagctacta aaatagttgc tattttagat tttccattaa
     2221 actcttctac ttcttcactt tcaaaacctt ttagttcttc tctaaacatt cctttgttaa
     2281 tgtctctttt caatgtataa aatgccacag atatggttac gattcccatc acccaaaaca
     2341 aaatcatacc atcatttata acatatgata ctgcaactcc tgctgcacca gctgttatac
     2401 ttggagctcc ttgaatgaca aagtctgtag ataaggcaag accatgacca aacaaattta
     2461 atgctactgc aacaccaatt gctggtagtc cagctctcat agcaacaggt agaaatacag
     2521 ctccaactaa ggcaactgct ggagttggcc agaaaaacca agaaagaact aacataacaa
     2581 atccagttac aaaaaatgct gtattagaat tttttataac ccttgaaaac ggtcttacca
     2641 tatactgtat agcattattg gcttctaagg ctttagataa tgcaaccata atagctataa
     2701 ttaagataat tggtcccaac tcatttaaag atataataaa agagttaaac actgctgtta
     2761 tagcaccaaa aatatttttt tcaaagaaca gtcccataaa aaaaacacct aatatacatg
     2821 gtactattgt atcctttttc ataatcatag taattaatat aattaccata aatagaatgt
     2881 aaatataatg tgctcctgtc aaaaccatat tattatgtcc ccttttttta agataataat
     2941 attatatggt tttgtaagta aacttgttac ttgcctagag ttacacaata tcgaaccttc
     3001 cataactaaa gtcacagagt tcctgcttca tagaattttg cataatcaat tacacaatat
     3061 ataatttaaa tttttatgat tcttttgctg gaatagatat ttcaatagca aattctttat
     3121 catctgagtt tacatttata cttccattat gatatgcaac tattttctca caattcttta
     3181 taccaattcc aaaactttct acattcttag aaaaattgct tactctattt ttttgtataa
     3241 ttaataattt agaatcctct actttaatta aaaatactat aggtttatct ttcctagcat
     3301 attttattat attagaacat aaattatcaa atatccttct aaattgtgta acatctacat
     3361 aaatatgata ttggttataa agttcatttt ctattgttat atgaaatcct ttctcctcca
     3421 atgtatatga actatctaaa agtaattgaa aaataaattc attagcattt attttttcat
     3481 acccttttga aacttcctct ccagaattaa caaaatgggt aaaaaggtca tctgttaaat
     3541 cttttatttg atatgcttta gaccttacat tttttatata ctgttccaat tgctcaggac
     3601 tattatattt atggtcttta attaaatcta aataacaaat tactgatgtt aatggagttc
     3661 taacatcatg tgatatcgaa gttactatat ctttattttt attcttcatc tctcgttctt
     3721 ttataagttg cttctccaat gtattgctca tctcattaat actagatgca agtgatgata
     3781 attcatcatt accttttacc gggattctat attttaaact gccactctcc actattttta
     3841 tacccatttc tattttcctt aggtacttta tctttctact aaacaatagt aagaatgtta
     3901 taataaatgt aagtgccata aatgtaatag aaattgcttc tataaaattt tgaaacctaa
     3961 atctaaataa agctactaca attatattag ctttaatatc agaaaattgt atttcataat
     4021 ttctagccat tggactgcta taaaactcat tttcctcact cttatcaggt acttcattag
     4081 gatagtataa tgtatcataa aaaaccctat ttcctttata tatcttaata gaaacaaacc
     4141 aattatcact attccatttt gctatatcat ctccatcatc cgctctgata ccattgttat
     4201 tcacataagt ttgaaaatca tccataagtc gatttacttc tcttttaaaa tatactttcc
     4261 cttctgatac atcttttact atagattcaa cagcagagta aaatggtata tttaaaatga
     4321 aagccactgc agctgagatt actacataca tcaatagtat aactcccaac ttagatggct
     4381 taattttgct aatcaatata ataccccttt ccccatgcgg tttttatata ttgcggattt
     4441 ttttggtctt tttctaactt ttttcttaaa tttctaatat gaaccattat tgtattattt
     4501 gctgtataaa aatactgctc atcccaaata gtttcatata aattttcaga tgagaaaact
     4561 ttttttctat ttttcaacat aagaactaat atactatatt ctatagttgt caaaaacaca
     4621 ggttgtccat ttagtgtaac atcttgagta tctaaattga cacataactc ttttgttttt
     4681 aatatatttg gctctttttc cttttcctta ccctggtata tataataacg tctaagtaac
     4741 gacttaaccc ttgaaattaa ttcagaatat gaaaaaggct ttgataaata gtcatctcct
     4801 cctgctgaaa atccaataac tttatcagaa tcttgagttt ttgcagttaa aaataatatt
     4861 ggtgctgtcg ttttttctct aatttcacta catgcaacaa atcctgtttt tacaggcatt
     4921 acaacatcca aaataatcaa atcaatcgta ctatctgcta agtttacagc ttcttctcca
     4981 ttcgttgcca taataacttc ataaccttca ccagttaaaa gtatttctac gatttctcta
     5041 atttcaggat tatcatcagc tactaaaatt ttttttggaa tttctttatt catcaccaag
     5101 ctcctttttt tgctatattt cttaaatatt tcaaactata taaaatatta tactttatta
     5161 gcaaaagctt agcaatgtca cacgttttaa taccttctac tttggtaaac atgtttaaaa
     5221 cttaattaaa atgtatacac aaattgacat attaataaaa ataatctata taatattatc
     5281 ttacataagc aacttttttt actgatttta ctttttcaga agattttaca tgtactatta
     5341 tacatattaa tccacttata gctacaaatg cgtaaaaact tattcctcta ctaagtaaca
     5401 ttgccggaac taataagtga ccagggaaaa atatcttaaa taatgtcata aaactatttt
     5461 cagaagctcc aacagcccct ggcaatggta atgatgaaac agcaatatta agcaaggatt
     5521 gtatcgctaa tatctcaaac acagtatatg ttgatagatg aaaagctttg tatacaaaga
     5581 aaggaactaa aaacatagct gttatttgta taatagttat gactaaaatt tggactacaa
     5641 gctttggatt ctgctttata tattgcgcac ctttaacata ctcttcaatt tgagatgtta
     5701 taacttttct tgaactttca acatctttta ttattcttaa cttacctaat aagtttgtta
     5761 ttgatattat aaaattattt acaagttttt ttgaaaatat aattgcaatt actccaacaa
     5821 caagagcaac atttgttatt acaccatata ttagtaaaat gttcataccg cttacattat
     5881 ttaatataaa ctcacgttcc ataataaaca ttactccact atatgccaat atgactactt
     5941 gatgaataac aacaatgact aatagtataa gtgaagaata tgatatattt atatcatctt
     6001 ttttcatata ataaacttgt gctggttgac cacctgatgc tgaaggcgta atagaactaa
     6061 aataaaatcc aataaaagca tatcccatac attttaaaag agttgttttt tgtcttaaag
     6121 ctttcatacc taaatatata tttatacctt cacaagcaac atatataaac atcattataa
     6181 atccaattag tataaacata gggcttgcac cttttaatga ttttatcaat gattcaactg
     6241 actgccctct aaaaacaaaa tatcctgtta tgcccattag taaaactaaa aaagctacac
     6301 tacctaggca tttatttttc ttttcagttt tacttatcat aactacatcc tttctggtgt
     6361 tacgaacttt aaaccactgg ctttaagttt tggaatagct acctttaagg cttcaatagt
     6421 acgctcaggt gcacctactg caccacctga attttcacct gcatcatgta aacaaattat
     6481 agaattttct tttgttctac tcattaattt gtttataatg atgtctactg tactattttt
     6541 gttccaatct tctgccatta catcccataa ggtcattttt agattatatt ttttaacaaa
     6601 caaatttgaa aatatattag tatgacccca tggtggacga tagtagctta catctacacc
     6661 taaatttttc attatattcg tactttcaat aaaatctttt ttaacataac tataactata
     6721 aagccatgcg tttttgtgtt ccaatgaatg taaaccaatt atatgatttt ctctcaacat
     6781 tctttttata atttcaggct ctttttcagc attctttgct actacaaaaa acatagcttg
     6841 tatatcattt tcttttaata cgtctagtaa tttttctgta tatctcttat ctggaccatc
     6901 atcaaatgta agtgcaattt cattttctcc agccatattt tttaatacct ctttatttaa
     6961 tagtttattg tagtatgtag gtataattga atggactaaa aaaatcaatg caacaatgat
     7021 aattaaacct actacataca ttccctacac tctccttgtt catcaagtga aaataaaata
     7081 ttttctaagg tattattatc aaactgtttt tttaattctt tcatgttact actcatttct
     7141 ttaagagtaa catcatcata aattaaatct tttatctcat ctacatagta ttttttattt
     7201 ttaccaagta ttctcccaat ttcattattt aatataaaac tagcattatc tatctcttgt
     7261 tgtaaaaatg gattaaatgc caatattgga agttctgaat aaattgtttc gaatagtgtt
     7321 attccacctg gtttagaaat aataaggtca gcacctttca tatatttata tacttcattg
     7381 gtgtaaccta ctacctctat attttcatat ctaccatata gtttatagta cattttttta
     7441 ttattaccag ttattacagt agtctttact ccttctaaat catttaactc tctgtagaat
     7501 tgttctgatt taggtagtaa tcctaatcct ccacccatta taagaatatt ttttttagtt
     7561 gagtgattta tatgttctat tcttttaaat tcttttttta ctggaatacc attaacttta
     7621 atcttagatt catctattcc tttaaaaaca agttcttcct tagtggattt actagctact
     7681 aaataacaat ctgtattttt actaatccat tctgagtgac tacttatatc tgttatgcat
     7741 gtaattaaag gaaggtttga gttatacttt tccttatatc ttgatactaa ttgagaacaa
     7801 aatggaaaag ttgaaataac tactgttggt tgtacttcat taagcaattc atctaattta
     7861 tttaaaaaat aatctgaaaa agtaaatttt atatctttct ttccattttc agcacactta
     7921 taaaacaaat tataaagact acttccccga tttacaagaa tagaaaaagt cttgtacatt
     7981 ttatctgaat agttaggcat aatatattca aatatatctt taattgttat ttctgaatta
     8041 tcaaattttg cttttatatc ttcacttaaa gaatttgatg ctgaataatg acccatacca
     8101 aattttcctg taagaattaa taccttcata agattccctc ctaattaaag cttttctttt
     8161 catataatta ctatacttat aagtatagac tttaaatctt tagaaaaacc tagtataatt
     8221 cttaagaaat cttaaagttt taattaaaaa atacaaagag accatctaaa aatagagata
     8281 accgtatttt ttattattta aataacacaa atatgttcga ttccatacaa aaaagagtgt
     8341 ctcatgaact aaaaagttca ttttgagaca ccttttttat atctacatgt attcaaatac
     8401 atacacaatc attgtaatta tttaaatgta ttttatttag ttgttcttta taattaatcc
     8461 ataatattat attcttcata taaaggatat ttttcagtta aaccaacaac caatgttcta
     8521 gccttagatt cttcatcata agttaaacat aggtctatag cttcagctat tattgccata
     8581 tcttcttctt tcatgcctct agtagttaca gctggagtcc caagtcttat tccactcgta
     8641 acaagagcac catttggatc aaatggaatt gtattcttat tagctgttat gtaagcatca
     8701 tccaatcttt tttctgctgc tttaccagtt atatttttat tagttaaatc taatagtatt
     8761 aaatgattat ctgtacctcc agaaatcaat ttaaaatctc ttttaataag ttcttcagct
     8821 aaggcttttg catttttagc aacttgtact tgatattctt taaactcatc acttaaagct
     8881 tctttaaagc aaactgcctt agaagcaatt atatgttcaa gaggtccgcc ttgaatacca
     8941 gggaatattg ctttgtctat atctttagca tatttttctt tgcaaagaat aacaccccca
     9001 cgaggtcctc ttaaagtttt gtgagttgtt gttgtaacaa aatcagccac ttcacatgga
     9061 ttctggtgaa gacccgcagc aacaaggcct gctatatgag ccatatctac cataagcaat
     9121 gctccaactt catcagctat ctctctaaac tttttaaaat ctatttctct aggatatgca
     9181 cttgctcctg caactatcat tttaggtttt acttcatgtg ctatctttct aacttcatca
     9241 aaatctataa gtccactgtc tttatctata ccatattcat aaaatttata atactgacca
     9301 gaaatattaa ctggagcacc atgagttaaa tgtccacctt gagataaatt cattcccata
     9361 acaacatcac ctggttttaa catagcaaaa tatacaccaa tatttgcatt tgctccagag
     9421 tgaggttgaa cattagcatg ctctgctcca aatatttttt tgattctatc tattgcaaga
     9481 gtttcaactt catctatata ttcacaccca ccataatatc ttttgcctga gtaaccttct
     9541 gcatacttat ttgtaagatg acttcccata gtttccatta ctggaactga tactatattt
     9601 tcagatgcta ttaattcaat atttctttgt tgtcttttta actctctttt catactttca
     9661 taaatctcag gatccgcttt tttcaagttc tcaaacatat tactcctcct agttattatt
     9721 taaattttat ttttacagac atttcataat tttcgttttt atcaatatga taatttatta
     9781 ttatagtcct ttagcaatta atagtcaagt gttttgccat ttttccatat ttaacataat
     9841 tactttcttt atttttaagt taaaatataa taaataatat tgtctaattc ttttaattgg
     9901 atataattta aatgtgacta ttttgaaaca aaaaaaagat aatatattta tatagatatt
     9961 aaggaatatt acttaaaaat ttgatataat ttcaatatta cgttttttaa tttggaggtg
    10021 tatttatgaa tcaaaatgag gaacttgaat ataagaaaaa tattctaaaa ctagctctat
    10081 ttgtagggga acttatgata aaaaatggag ctgaaacatc tagggttgaa gacagtgtcc
    10141 ttagaatatg caggtcaaga gggttttatc atgtaaatat ttttacaact cctacagtaa
    10201 taattatttc agatgagaaa tttgatggct tttcgtttat gaaaacgatt caatcaagag
    10261 gtataaactt aaataagatt tctttattaa atagtttttc tcgagaattt gttagtgaca
    10321 aagagtatga tattgataat gctattgcac gtctttatga aatacaagat gtaaaacctt
    10381 atcctttatg gttattttac tcttgtacag gaattgcatc tgcttgtttt gcttgtttgc
    10441 ttggagggaa ttatgtatta aattttatac ttacgtttat aatagcaatc tttgctgcaa
    10501 ttacttatga taaaacgatg aagataagtg caatatctgc gttttcctgt ttagtatctt
    10561 cttttttaat tgcactttca ggagttctgc tagttgaaat aggcatttta gatgacccta
    10621 aaatgcttat agtcggcgcc ataatgccac tactcccagg agttgctttt ataaaagcta
    10681 ttagagattt aatatcaggt aatttaatat caggaatttc tcgtgctttt gacgctgcta
    10741 tgattattat tgcaattgct tctggagttg gttttgttct tgatacatgg tatagaatag
    10801 gaggtccttt ctaatgactg atatgtcaat gattttacat tttttatttt caggcgttgc
    10861 aactgctgga tttgctgttt tctttaatgc tccactctat ctacttcttc cagctggaat
    10921 aactggagga attggttgga ttgtatatgt ttatttattt aattttacta caaatgccgt
    10981 ttttgcaggt tttatagctg ctgctttggt ctcagcatgc agtgagactt tggcaagaaa
    11041 actaaagcaa ccagctattg tgttcgtaat tccagggatt cttcctctaa taccagggat
    11101 tgggctatat aatacaatgt tgtctttgat tcagaagaat tattctttgg ctatgtcaaa
    11161 aggtacagat gcgctatttc taagtgctgc aatagcactt ggtgtattag ttgtaacttc
    11221 ttttgtaagg acattgaatc ttttgaaaat cagaaaaaac tttattcctt acagaaaatc
    11281 aaaataaaaa tataaaaata gcaatgtact taaactttat aaatacacat tgagtattta
    11341 tttaaaaagc acattgctat tttttaatta taattatctt aaaggtcaat taatattcgt
    11401 gttattactg gtataaactc tctactgtct ctgaattttt tgtcattctt ctttaagtat
    11461 aaataacaaa ttattgtaag acaaattcca actagtccac ttatagcttc aactccagtt
    11521 ttcagtccaa ttgcccccaa tatataataa gtccctattg ttcctacaag aagtgctata
    11581 agaggtatta tataagccat tacagctgct tttaatacat tcatattatc catactcact
    11641 tctacatggt caccaacttt tgcaccaatt gtgttgtcta cttctactaa tatttctttt
    11701 gactctgctg aatttgtagt agcacatttt ccacaagcag cacaagctga atgtctttgc
    11761 atttttaact ttgcagttct atcatcaaca atttccataa taaatccttg ttgattcata
    11821 actaaccgcc tccttatttt gtaaatttat ttatagtatc cataagctct tttatcatct
    11881 cttcagtttt atcagcatca tcatgtttga tactttctgc tacacatcct tttatatgat
    11941 tctctaagat aattgtgcct actctattta tagcagacct tatagcagat atttgaacca
    12001 atatatcaac acaataacgt tcttgttcga ccattttttg tatcccctta acttgacctt
    12061 ctattctttt taatcttttt atgagagctt ccctctcatt gtttggtgct aattttctct
    12121 cttccataaa ttcacctcat actctaatga tgtcttatgc aattatgctc tacttaccaa
    12181 cttgtttaaa atataataca gttattatta ctataaatac caaaattgat attgcaatag
    12241 acctgctttg attcttagtt tgttccatac ttaactttcc attcatctta aatcctgcca
    12301 atgggaataa aaatataacc agtgcatcaa ttccaagtac aacttgggct gcaatagtcc
    12361 atttgctcaa aaagcagaat attccaccca cacctaaaag aacctctaat atatttataa
    12421 acttatatgt aggttttttt gctatatatt cttcaatttt ttccttacat tctgcacgac
    12481 atggatatat attcgtattg tcatcatatt ttatagataa attattattt ggtgacttta
    12541 tatccctatt acagtaaaga cattttttca ttttatattc tcctctagta ttccttaaaa
    12601 aattttgcct aaattattat acgctataat actattatac caaatagata caaaaaacaa
    12661 aaataaaagg aataatttta gctatttctt ttatttcaat gtgtagtcta taataaattt
    12721 ataataacaa ttaaataata ttatttagtc taatttaatt gttatgtaat ttaaaataag
    12781 tcaaatttgt atgagaggtt aaaatgaaac aaatagaatt agctattgta agtttaaaaa
    12841 aagacgctgg agaaatttat gaaaatcaaa taaggcaatt cttaggtgat aatctaaaga
    12901 taaatttata ctcatttgaa gagggaaatt taaagttttt taaagaaaaa cttatactcc
    12961 tatctgctta cttaaaatat gatgaaatag taaaattatc tcactatgat gctcaaatca
    13021 ttgttcccaa gctgacattt gaaaagaaca gtattgatat gatatccaaa ttagaaaaag
    13081 ataaaattat ttatgtatat aacttaagta aagatatggc aatagaaaca atttctttaa
    13141 tacatagact aggaatagac aatatcaata tcctgccctg ctatccagag attgagttta
    13201 ctccctcaga tgctataatc ctaactcctg gtgaaaaaat cttgcctaag tttaaaaatt
    13261 gtgaagttat agatttaaaa tacagaataa tagatttaag ttgtattgtt gaaattgcta
    13321 caaaaaccaa attaaaacac ctaataaaag atgagctaat aaaaaaatac gtagaaaaaa
    13381 taattcctac aagttatagt acaggtgaat tattattaga tgcaaataaa tttgaaagac
    13441 aatttgatta aaaaatagct acg
//
LOCUS       DAFNNB010000107        13447 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0108, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000107 DAFNNB010000000
VERSION     DAFNNB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13447
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0108"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            131..1060
                     /gene="glsA"
                     /locus_tag="L3M60_002535"
     CDS             131..1060
                     /gene="glsA"
                     /locus_tag="L3M60_002535"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBN6227189.1"
                     /translation="MNTLDETLLKEIISSNKKYTNYGQVASYIPELKNARRNDLGICI
                     IDSENNLYSAGNCSTKFTIQSISKPIVLAMALMDNDWEDVFSNVGMEPSGDPFNSIMK
                     LEINDTKKPCNPMINAGAIVTTSLINGSCLEEKEERMLSFFRKLAKNDNIGINYDVYK
                     SEKMTGDRNRAMAYLLKSDGFIRGNVEDVLDLYFKQCSIEIDSVDLARIGINLANYGV
                     DIENGEHLMSEMVSRIVKTFMMTCGMYDASGEFAIKVGIPAKSGVGGGIMASVPGRMG
                     IGVYGPALDKKGNSVAGVKVLEELSNKLKLNIF"
     gene            1158..1721
                     /locus_tag="L3M60_002536"
     CDS             1158..1721
                     /locus_tag="L3M60_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227190.1"
                     /translation="MKEVLVLRDLECIKAIAHPRRIDILKAFDKSPLSAKQLSQLLEE
                     PHAKINYHIKMLYKVGILELVEEKIKSGIVEKYYYPSAKNVVIGNRILNFSLDNGEEK
                     EELYISKFENMSEVFYKAAEEDVLENENIVDYHDISLTHDELVELSDTMKSKIDEILN
                     KRQHNVEGSKYDIAMVIVPTLEEECPS"
     gene            2204..3040
                     /locus_tag="L3M60_002537"
     CDS             2204..3040
                     /locus_tag="L3M60_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBN6227191.1"
                     /translation="MIKLGTHLSIAKGFANAAETAVYIGANTFQFFSRNPRGGNAKEF
                     NEKDIIKFQEIRKANNFAPLLAHAPYTMNLGGTKDDVYEFATRVIDEDIRRMDSLGIE
                     YMCFHPGSHVGGGVDFGIDRIVKGLNNAIKGDENITILLETMSGKGTEIGFKFEQLKS
                     IIDKIEHKEKIGVCLDTCHIFSAGYDIVNDLDGVLEEFDKVIGIDRLKTVHLNDSMMP
                     FGDKKDRHAPIGEGKIGLKALIDFMEHPSLKHLPFFLETPFDDEGHKRELKMIKEILS
                     SK"
     gene            3323..4318
                     /locus_tag="L3M60_002538"
     CDS             3323..4318
                     /locus_tag="L3M60_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBN6227192.1"
                     /translation="MRNLGNTNMKIKRVGFGGIPIQRITQDDTNLVINELEKQGINFI
                     DSARGYTISEEAIGIAIEGKRDKFFLATKSMSRDYDSMKRDVEISLNNFKTDFIDLYQ
                     FHNVKEEEYDNLFKDKMAYSALLEAKEQGKIKHIGITSHNLNTIEKAIEDGKFDTIQF
                     PYNIVEGQADDVFKKAHEKGIGIIVMKPLAGGALDNATLAIKYILSKDYIDVVIPGME
                     SVEQVRQNVAVLENLVLDEKDNKEIEEIRNSLGKKFCRRCEYCMPCAVGINIPLSFLC
                     EGYYTRYGLKEWAKEKYEVMDVKPTECIDCGLCESRCPYELPIREMLKTVVEKLG"
     gene            complement(4552..4956)
                     /locus_tag="L3M60_002539"
     CDS             complement(4552..4956)
                     /locus_tag="L3M60_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBN6227193.1"
                     /translation="MIKITIPGRPISKSNFKLHNINGQAWMPSKGKYSKYLAYENMIA
                     GFINQQYQGETVEENLITVLKLFFPNKRMGDLHNYPKSICDGIEKSGIIKNDKQLKPV
                     LLFDFIDKDNPRVEIELYPVSKYNISYNIFEK"
     gene            5714..6628
                     /gene="queG"
                     /locus_tag="L3M60_002540"
     CDS             5714..6628
                     /gene="queG"
                     /locus_tag="L3M60_002540"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBN6227194.1"
                     /translation="MNNRELKEFCNLIGLNCVGVAGVDKYDNLENILKDRYKKGYLTG
                     MEEPIIENRINPRNIMEDANSIIVCAFPYYINRQEKNKNSNLSKYCHGKDYHIVVKKF
                     LQQICDYISKKVTDFKYKIFADNGPLVDRYLAYLSGIGYFGINNNIITDEYGSYVFIG
                     YIVNNYKFEEDKPLEKSCFKCGKCVKYCPGNAILGNYDMNPKKCLSYITQKKGDLSQE
                     EKAVLKSGKKVFGCDICQEVCPHNVEIPTTHILEFKENIIDYLDYDEIQNISNKEFKR
                     RYGDRAFSWRGKNIIKRNMEIILDKYND"
     gene            6734..8971
                     /locus_tag="L3M60_002541"
     CDS             6734..8971
                     /locus_tag="L3M60_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6227195.1"
                     /translation="MKYNNTSEINPLDCILGQERAVEAMELGLKINNPAYNIYIAGES
                     GTGKSTYALKVLNEYASKKNTHKDWCYIYNFENPREPIIVELEKGFGRELKKDMEKLI
                     ESLLEDFKNAFESENFEIEKNKLLDEYEIEKDMLLKQIKKYGEEKGFKLKQSKMGIVF
                     IPLKEEEDESDESDEEFYKAKRELENMAIQVVYKIRNLEEIAEKAVLELEEEIAKLVV
                     EPHIKRLCEKYENYDKIQDYLENIKKDIIEYMYLFYLDEEELKDKYDKEHFIKYKINL
                     FVDNGSSKNKESAPVVVEINPSPANLFGKAEYDYANGNIKTDFTKLLSGAFHRANGGY
                     LVLYADQLLKYTMSWEILKKTLQTKKVILETQTAIKPENMPLDVKLVLIGSHYIYDIL
                     YRYDEDFEKYFKVFVDFDSEMDKNEGNEEGIARFIASQCDKNNLRHFTKSAVEEVVKF
                     STRLSGDLEKLSTKFNKIMEVVIEGSAYAEFRNSEYTEQCDVKKAISEKRKRINRVET
                     HMDESIENGFTLIETEGRKVGVINGLSVLSTGEYSFGRASRITATTSPGSKGIVNIER
                     EVNMSGSIHNKGVLILGGYLSENFAQDLLLSLNAYVCFEQNYGGIDGDSASSAELYVL
                     LSSLSGVPIKQNIAVTGSINQKGDIQVVGGISEKIEGFYSICKTKGLNGEQGVIIPRK
                     NQRNLVLSDEVNDAVQDGKFKIYTVERVEEAIEILTDVKFEEIRKLVKEKLISFSKIQ
                     TVSKE"
     gene            9169..10026
                     /gene="nth"
                     /locus_tag="L3M60_002542"
     CDS             9169..10026
                     /gene="nth"
                     /locus_tag="L3M60_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBN6227196.1"
                     /translation="MKNVNLKEINKKEVIDKEEINKKGIDKKDVTEKEKINKKSEHDK
                     KTKNSSSSKDTQKLSKGIKSKSKIKNESANEKDVNKILDELEKLYPDAKCELNYGTAF
                     ELLIATILSAQCTDVRVNKVTSELFKKYNTAKDFANLSIEEISKEIKSCGLYKSKSQK
                     IKDTSEQLCELYDGEVPDSLEKLIKLPGVGRKTAGVVLSNAFNHPAIAVDTHVFRVSN
                     RIGIVDEPNPQKTEFALMEAIPKERWSHSHHVLIFHGRRMCKARNPECASCPIKEDCN
                     YYKELNETK"
     gene            10220..10690
                     /gene="trmL"
                     /locus_tag="L3M60_002543"
     CDS             10220..10690
                     /gene="trmL"
                     /locus_tag="L3M60_002543"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBN6227197.1"
                     /translation="MSLNIVLVEPEIPQNTGNIIRTCAATGSTLHLVKPLGFSLDDKH
                     LKRCGLDYWDIADIQYYDSFEELQEKYPNSKYFFSTTKAKQSHSDVKYEENCFIVFGK
                     ETKGLPETLLKANMDTCIRVPMLDVEKARSLNLSNSVAIVVYEALRQIGYPNMR"
     gene            10725..11573
                     /locus_tag="L3M60_002544"
     CDS             10725..11573
                     /locus_tag="L3M60_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HBN6227198.1"
                     /translation="MKNLKIICDSLSDVPKDLLDKYDIDIVSLTVILEEKEYKDRIDI
                     SGDEFYKRLREENVYPKTSQATYAQFKEIFEKYTNEGREILYIAGSATATGTYQSAVM
                     AKNDVEGKIYTYDSNLLCMGTGLLVIKAGELASEGKTVDEILPVLDELKEKAFVIFSV
                     DTLEYLQKGGRISSTKAAIGSILNIKPILEIRDGLVSQISQVRGKKNVISKMMELIKS
                     NCGDDLSDQIVYIGYSDDFKEKEQLSKVMEDELRPKEIRFFQIGVCIGAHAGPGVTGI
                     ICLKNK"
     gene            11861..12865
                     /locus_tag="L3M60_002545"
     CDS             11861..12865
                     /locus_tag="L3M60_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6227199.1"
                     /translation="MRFKRILIFILTVAMTAGMLVGCDSNQSNNPSSSKDNKSDISDK
                     SNSKKSVSMILDIEGTNNEAMNNSALLALNNAQKKLNIDTNKVESDDSSTFSNSIDIL
                     CNDNYDLIIAVGARFAKPLEMVAKKYPKQQFAIIDYEYDKQPSNITSISYEDNKSGYL
                     AGLIAGKMTESDKVGFIGGTKSSSRDKFETGFKEGVKFSNSSIKDISVEYADVFKDRK
                     SVESIAKKMMDNGVDIIFSTTEDDSKAVIGAVRTKNKKVIATNKDQHELAPENVISSI
                     VKDFENSTYNLIQSFVKGNYKGGKVIENTVKSGSTGLAQNSSQNIPPDVLEYVSKNNK
                     "
     gene            12991..13326
                     /locus_tag="L3M60_002546"
                     /pseudo
     CDS             12991..13326
                     /locus_tag="L3M60_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
BASE COUNT         5449 a         1261 c         2524 g         4213 t
ORIGIN      
        1 tttaaattgt ttgaaaaatg aattgattct tgtagaaaat aactttaaag tataattttt
       61 tttaacactg ttttattata gtacaaactt gtgctaaaat agaatatata acataatata
      121 agaggtgcat atgaatacgt tagatgagac attattaaaa gaaattataa gttctaataa
      181 gaagtatact aattatggac aagttgctag ttacatacca gaattaaaaa acgctagaag
      241 aaatgattta ggaatttgta tcatagatag cgaaaataat ctttatagtg ctggaaactg
      301 tagcacaaag tttactatac aaagtatttc aaaaccaatt gtgcttgcta tggcacttat
      361 ggacaatgat tgggaagacg ttttttcaaa cgttggaatg gaaccaagtg gagacccatt
      421 taattcaata atgaaacttg aaattaatga cacaaaaaaa ccctgtaatc caatgataaa
      481 tgcaggggct atagtaacta cttcccttat aaatgggagt tgtttagaag aaaaagaaga
      541 aagaatgctt agtttcttta gaaagttagc aaaaaatgat aatataggaa taaactatga
      601 tgtctataag tctgaaaaaa tgactggaga tagaaacaga gctatggctt acttattgaa
      661 aagtgatgga tttatcagag gtaatgtaga agatgttcta gacctttatt ttaaacaatg
      721 ctcaattgag atagattctg tagatttagc gagaattgga ataaatcttg caaattatgg
      781 agtggatata gaaaatggcg agcatttaat gagtgaaatg gtttctagaa ttgtcaagac
      841 atttatgatg acttgtggaa tgtatgatgc ttctggggaa tttgctatta aagttggcat
      901 tcctgcaaaa tctggagttg ggggtggtat aatggcttct gtgccgggac gcatggggat
      961 tggtgtttat ggacctgcgc ttgacaaaaa aggtaacagc gtcgcaggag ttaaagtttt
     1021 agaggagttg tcaaataaac tcaaattaaa tattttttaa aatattaaga aaggattttt
     1081 ttgacaacaa catataattt aatatataat taaattgtaa agaaatatga attaaataaa
     1141 ttttttagga ggggtcaatg aaagaggtat tggtattacg agatttggag tgcataaagg
     1201 ctattgcgca tccacgtaga atagatattc taaaagcatt tgacaaatcg ccattgtcag
     1261 caaaacaatt atctcaatta ttggaagaac ctcatgcaaa aataaactac catataaaaa
     1321 tgttatacaa agttggtatt ttagaattag ttgaggaaaa aataaagtcg gggattgtgg
     1381 aaaagtacta ttatccaagt gcaaaaaatg tggttatagg gaataggata cttaattttt
     1441 ctttagacaa tggggaagaa aaagaagaat tgtacatatc aaaatttgaa aatatgagtg
     1501 aagtttttta taaggcagca gaggaagatg ttttagaaaa tgaaaatata gttgattatc
     1561 atgatatttc tcttactcat gatgaattag ttgaacttag tgacactatg aagtcaaaga
     1621 tagatgaaat tttaaataaa agacaacaca atgttgaagg ttctaagtat gatatagcta
     1681 tggttatagt tcctactctt gaggaggaat gtcctagtta aagaattaat tatggcttaa
     1741 ttaaagatat aagattagag attgaacatc aaaatttact taataatcaa aaaatagttt
     1801 ttggtatggt aaagtaatga tttttaatct ctattttttt gtctctttat actattgtgt
     1861 ttgtaaatga tagatagtgt gtaagatgta ttcactatct aagaagtgct tagataatgt
     1921 atacaaagtt tgtaagatta taatatttga aattgattag aatgggtata attaaaatgt
     1981 aaatattaaa attaaaaatt aatataaatt aaatcaatgg tagaaatata ttaaaagagg
     2041 aatgaaatta gtggggatgg aacatccctt agagcctata aatttttagt gtaaaagtac
     2101 tataggaatc ttcatcttta gatgttggct gaaagtagct aaggtggagt agttcaaaat
     2161 taaaattgag tacaagttgt agtacaaata aaaggagatt aatatgatta aattaggaac
     2221 acatctttca atagcaaaag gatttgctaa tgcagctgag acagctgtat atattggtgc
     2281 aaatacattt caatttttta gtagaaatcc acgaggagga aatgctaaag agtttaatga
     2341 aaaagatata attaaatttc aagaaattag aaaagcaaat aattttgcac cactgcttgc
     2401 acatgcacca tatactatga acttgggtgg cacaaaggat gatgtatacg aatttgcaac
     2461 aagggtgata gatgaggata taagaaggat ggattcatta ggaattgaat acatgtgctt
     2521 tcatcctgga agtcatgtag gtggtggtgt agattttggt atagatagaa tagtaaaagg
     2581 attgaacaat gctataaagg gcgatgaaaa tataactata cttcttgaaa ccatgtcagg
     2641 aaagggaact gaaataggtt ttaaatttga gcaattaaaa agtataatag ataaaatcga
     2701 acacaaagaa aaaataggtg tgtgccttga tacatgtcat attttttcag ctggttatga
     2761 tatagtgaat gatttagatg gggttttgga agagtttgat aaggttatag gtatagacag
     2821 gttgaaaact gttcatctaa atgatagtat gatgcccttt ggagataaaa aagacagaca
     2881 tgctcctata ggggaaggaa agataggatt aaaagcatta attgatttta tggaacatcc
     2941 tagtttaaaa catttaccat ttttcttaga aactcctttt gatgatgaag gtcataagag
     3001 agagcttaaa atgataaaag aaatattatc tagtaaataa aattttattt aaacatgtaa
     3061 aatactccac tcttaacata attagggtgg agatacaatc atgaaatgta ataatttaaa
     3121 ttaatagata ggaataacga aaattgtata cgaattaact gggtataaag tatgatgata
     3181 ttatgaatag actgatataa aatataacta tattatgaat agtatgtaaa tatattgcaa
     3241 agattctcat aatttgttgt ataattaaag gtgacatatt tatattatat aaaaaattat
     3301 taaaagttag gtggggaaag atatgagaaa tttaggcaat actaatatga aaataaagag
     3361 agttggtttt ggaggtattc caatacaaag gattactcaa gatgatacca acttagttat
     3421 aaatgagtta gaaaagcaag gaataaactt tattgacagt gcaagagggt atacgataag
     3481 tgaggaagca atcggaattg ccattgaagg aaaaagagat aaatttttct tagctactaa
     3541 aagtatgtct agagattatg attcaatgaa gagagatgtt gaaataagtt taaataattt
     3601 taaaactgat tttatagatt tatatcaatt tcataatgtt aaagaagagg aatatgataa
     3661 tctttttaaa gataaaatgg cttatagtgc ccttttagag gcaaaagagc aaggtaagat
     3721 aaaacatatt ggtataacta gtcacaattt aaatactata gaaaaagcta tagaagatgg
     3781 aaaatttgac actatacaat tcccttacaa tatagttgaa ggacaagcag atgatgtgtt
     3841 taaaaaagct catgaaaaag gaattgggat aatagtaatg aagcctttag ctggaggtgc
     3901 tttagataat gcaacattgg ctattaaata tatactatct aaagattaca tagatgtagt
     3961 tataccaggt atggagagtg ttgaacaggt tagacaaaat gtggccgtac ttgaaaatct
     4021 tgtgctagat gaaaaagata ataaggaaat tgaagaaata agaaattctt taggtaaaaa
     4081 attctgtaga agatgtgaat attgtatgcc ttgtgctgta ggaataaata taccattgag
     4141 ttttttatgt gaaggttatt atactagata tggattaaaa gagtgggcta aagaaaaata
     4201 tgaggttatg gatgttaaac ctactgagtg tattgactgt gggctttgtg agagccgatg
     4261 tccatatgaa cttcctataa gagaaatgct gaagactgtt gtcgaaaaac taggatagat
     4321 tttgtttatt gaaatcggtg tagtaaattt actccgattt ctttttatta tatgtggtct
     4381 tatatacaaa tattaaattt acatagttat aggcattata taaatttaat atttgtttaa
     4441 tacttattaa atatttgcgt gagtatagat ataacacaaa tattatttgt tgtattagta
     4501 tagtttcaaa tactagagca tcaatataat ggcgaataac atacaaaatc tttatttttc
     4561 aaaaatattg taggatatat tatatttaga tactggataa agttcaattt caaccctagg
     4621 gttatcttta tctataaaat caaataaaag tactggcttg agttgtttat cattttttat
     4681 tattccactt ttttcaatac catcacaaat actttttgga taattatgta aatcccccat
     4741 tctcttattt gggaagaata atttcaagac tgttattaga ttttcttcaa cagtttctcc
     4801 ttgatactgt tggtttataa aacctgcaat catgttttcg tatgcaagat acttagaata
     4861 ttttcccttt gaaggcatcc atgcttgacc atttatgttg tgaagtttaa aattagattt
     4921 actaataggt ctacctggaa ttgttatttt aatcaaattt ttcaccatcc ttatacagta
     4981 tttattataa tctgtatatg gtttaaaagc aaattgttat ttaaagtgtt tttacagagc
     5041 ttatttgtga acttttccac tttggatgta gtgatataag acaagtgttt tatttacaaa
     5101 ttttaaaatc tgatatatgc tgaatatatt catattaggc tattataaac ttatcacaat
     5161 attaatgaac ataagtttag tgaagagaaa aaaagagaac aatataagtc aagtaaagac
     5221 ttaataaaca gtaatgtaaa tggaatatta aatataagaa aaaatcaatc ttcttacatt
     5281 aattatagga agaatgtcaa aatataacta atgatatttg aatttttaga atttagatta
     5341 aaaaccaaaa aggttaagtg ggtaaaaaag attgaataga ttaaaaaggc taggtgagta
     5401 aaaacattaa atagctaaaa aaggctaagt agataaaaga gttaagtagt taaaaaattg
     5461 aagatctaaa aaagactaag taggatagaa gtgttaagta agttaaataa aaaagactaa
     5521 gtagataaaa aaattaaata agtaaaaagg ttgaataagt aaaaaatact aagcagataa
     5581 aaaattaaat gggtaaaaag gttgagtata taaaaaaact aagtagataa aaaaattaaa
     5641 tgggtaaaaa ggtcgagtat ataaaaaagt taaataaatt aaaaggttaa atagattaaa
     5701 aaggaataat tttatgaata atagggaatt aaaagaattt tgtaatttaa taggattaaa
     5761 ttgtgtagga gtagcaggtg tagataagta tgataattta gaaaatatac ttaaagacag
     5821 gtataaaaaa ggttatttaa ctggtatgga agaaccaata attgaaaaca gaattaatcc
     5881 aagaaatata atggaggatg ctaactctat aatagtgtgt gcatttccat attatataaa
     5941 tagacaagaa aaaaataaaa attcaaattt atcaaaatat tgtcatggaa aggattatca
     6001 tattgtagta aagaaatttt tacaacaaat ttgcgattat atttctaaaa aagtaacaga
     6061 ttttaaatat aagatatttg ctgataatgg accattagtt gacaggtatt tagcttattt
     6121 atcaggaata ggatattttg gaataaataa taatatcata actgatgaat atggttctta
     6181 cgtatttata gggtatatag taaataatta taagtttgaa gaagataaac cactagaaaa
     6241 aagctgtttt aaatgtggta agtgtgtaaa atattgtccg ggaaatgcta tattgggaaa
     6301 ttatgatatg aatcctaaga aatgtttatc atatataact caaaaaaaag gagatttgtc
     6361 acaggaagaa aaagctgttt taaagagtgg caaaaaggta tttggatgcg atatttgtca
     6421 agaagtatgt ccacataatg ttgaaatacc aacaactcat attttggaat ttaaagaaaa
     6481 tataatagat tatttggatt atgatgagat acaaaatatt tcaaataaag aatttaaaag
     6541 gagatatgga gatagggctt ttagctggag agggaaaaat ataataaaga gaaatatgga
     6601 aataatttta gataagtata atgactaata ataagagtct gtttcatata ctttaatgtg
     6661 tatttacttt aacacttgtt gtgtgaaagt catattatat cattaaagtt aaatatgggg
     6721 aggtggggga atattgaaat ataataatac atctgaaata aatccactag attgtatatt
     6781 gggtcaagaa agagctgtag aagctatgga gctaggttta aaaataaata atccagcata
     6841 caacatatat atagctggtg aatctggaac tggtaagagt acttatgctc taaaggtttt
     6901 aaatgaatat gcatccaaaa aaaatacgca taaagactgg tgctatatat ataattttga
     6961 aaaccctaga gagcctatta tagttgagtt agaaaagggg tttggaagag aacttaagaa
     7021 agatatggag aaattaattg agagtctttt ggaagatttt aagaatgctt ttgaaagtga
     7081 aaattttgaa atagaaaaaa ataaactttt ggatgaatat gaaatagaaa aagatatgct
     7141 tttgaagcaa attaagaaat atggagaaga aaaaggattt aaattaaaac agagtaaaat
     7201 gggaatagtt tttatccctt tgaaagaaga ggaagatgaa agtgatgagt ctgatgaaga
     7261 gttttacaaa gctaagagag aactagaaaa tatggcgata caggtcgtat ataaaattag
     7321 aaacttagaa gaaatagctg aaaaagcagt gttagagctt gaagaagaga tagctaagct
     7381 tgtagtagaa cctcatatca agagattgtg tgaaaaatat gaaaattatg ataagattca
     7441 agattatctg gaaaatataa agaaagatat aatagaatat atgtatttgt tctatttaga
     7501 tgaagaagag ttaaaagata agtatgataa agagcatttt attaaatata aaattaactt
     7561 atttgtagac aacggaagtt caaaaaataa agaatcagca cctgttgtag tagagataaa
     7621 cccaagtcca gcaaatctat ttggaaaggc agagtatgat tatgcaaatg gaaatataaa
     7681 gactgatttc acaaaactat tatcaggagc atttcatagg gctaatggtg gttatttagt
     7741 actttatgca gaccaattac taaaatatac aatgtcttgg gaaattctta aaaagacttt
     7801 gcagactaaa aaagtaatat tggaaacaca aacagcaatt aaaccagaaa atatgccatt
     7861 agatgttaag ttagtactga ttggaagtca ctatatatat gatattttat atagatatga
     7921 tgaagatttt gaaaaatatt ttaaagtatt tgtagatttt gatagtgaaa tggataaaaa
     7981 tgaaggtaat gaagagggaa tagcaagatt tatagcatcc caatgtgata agaataactt
     8041 aagacatttt actaaatcgg cagttgaaga ggttgttaaa tttagcacta gattgtccgg
     8101 tgatttagaa aaactatcaa ctaagtttaa taaaataatg gaagttgtaa tcgaaggaag
     8161 tgcttatgca gaatttagaa attcagagta tacagagcag tgtgatgtta aaaaagcaat
     8221 aagtgaaaag agaaaaagaa tcaatagagt ggaaacacat atggatgaat caattgaaaa
     8281 tggatttact ctaatagaaa cagagggaag aaaagtagga gtgataaatg gtctgtcagt
     8341 cttaagtaca ggagaatact cttttggtag agcttcaagg attacagcta ccactagccc
     8401 aggaagtaaa ggtatagtaa atatagaaag agaagtaaat atgagtggct ctatacataa
     8461 caaaggagtg cttatattag gcggctatct ttctgagaat tttgcacagg atttgctatt
     8521 gtcactaaat gcatatgttt gttttgaaca aaattatggt ggaatagatg gagatagtgc
     8581 atcttctgca gaattatatg tactattatc atctcttagt ggagtaccaa tcaaacaaaa
     8641 tattgctgtt acaggttcta taaatcaaaa gggtgatata caagtagttg gtggtataag
     8701 tgaaaagata gaaggatttt attctatatg taaaaccaaa gggttaaatg gtgagcaagg
     8761 tgtcataatt cctagaaaaa atcaaagaaa tctcgtttta tcagatgaag ttaatgatgc
     8821 agtgcaagat gggaaattta agatatatac tgttgaaaga gtagaagagg ctatagagat
     8881 attgacagat gttaaatttg aagaaataag gaaactggta aaagaaaaat taatatcttt
     8941 cagtaagata caaactgtgt caaaagaata agtttattat agaatttaat gggtatttat
     9001 gtataataaa aagcaagaac tttaataagt ttttgctttt tttgtgaaat ctatataaat
     9061 taaattctta ttttgtatgt tttatatatt gatgtttatt ttttgttgat atgattaaac
     9121 tatgtataaa tagcgtttta taaattagta ttttaaaggg ggataattat gaaaaatgta
     9181 aatctaaaag agataaataa aaaagaggta attgataaag aagagataaa taaaaaaggt
     9241 atagataaga aagacgtaac tgagaaagaa aagataaata aaaaaagtga acacgacaaa
     9301 aaaacaaaga atagtagttc tagtaaggat acgcaaaaac taagtaaagg aattaaaagt
     9361 aagtctaaaa ttaaaaatga gtcagcaaat gaaaaagatg taaataagat tttagatgag
     9421 ttagaaaagt tgtacccaga tgctaaatgt gagttaaatt atgggacggc ctttgaactt
     9481 ttgatagcaa ctattttatc agcacaatgt actgatgtaa gagtgaataa agttacttca
     9541 gagttgttca aaaagtacaa tacagctaaa gactttgcta atttgagtat tgaagaaatt
     9601 agtaaggaaa taaagagttg tggtctttac aaaagcaaat ctcaaaaaat aaaggataca
     9661 tctgaacagc tatgtgaatt atatgatggt gaagtgcctg atagcttaga aaagcttata
     9721 aaattacctg gtgttggaag aaaaacggca ggggttgtac ttagcaacgc ttttaatcat
     9781 ccagcaatag cagtagatac tcatgtattt agagttagca atagaatagg aatagtggat
     9841 gagccaaatc ctcaaaaaac agaatttgca ctcatggaag ctataccaaa agaaagatgg
     9901 tcacactcac atcatgtatt aatttttcat ggaagaagaa tgtgtaaagc tagaaatcca
     9961 gaatgtgcaa gttgcccaat aaaagaagat tgtaattatt ataaagaact taatgaaacg
    10021 aaataacttt tagaaagtaa gttgtacaag agtcattttc tgatataata atacttgtga
    10081 aaatgttaga taaaaaacta ttatatacat gaaataaaaa ttattattaa cattatttta
    10141 tataatagta taatattatt gttattctat ttataagata aaatttgata aatagaagca
    10201 atatttaggg aggattaaaa tgtcattaaa tatagtgtta gtagaaccag aaatacctca
    10261 aaatacagga aatataataa gaacctgtgc agcaacaggt tcaactttac atttagtaaa
    10321 gccactgggt ttttcattgg atgataaaca cctaaaaaga tgtgggcttg actattggga
    10381 tatagctgat atacaatatt acgatagctt tgaagaatta caagaaaagt atccaaacag
    10441 taaatacttt ttttctacaa caaaggctaa acagtcacat tcagatgtga aatatgaaga
    10501 aaattgtttt atagtatttg ggaaagaaac aaagggatta ccagagactt tgttgaaagc
    10561 aaatatggat acttgtatta gagtgcctat gttagatgtt gaaaaagcta ggtcattaaa
    10621 tttatcaaat tcagttgcta ttgtagtgta cgaggcttta agacaaatag gatacccaaa
    10681 tatgaggtag tttaacaagc ctaaatgaga aaggagcata cttaatgaaa aatctaaaga
    10741 taatctgtga cagtttatcg gacgtgccaa aagatttgtt agataaatat gatatagata
    10801 tagtttcgtt aacagttata ctggaggaaa aagaatacaa ggatagaatt gatatatctg
    10861 gagatgaatt ttataaaagg cttagagaag aaaatgtata tccaaaaact tctcaagcta
    10921 cttatgccca atttaaggaa atttttgaaa aatatacaaa tgagggtaga gagatacttt
    10981 atatagcagg gtctgctaca gctacaggga cgtatcaaag tgctgtaatg gcaaaaaatg
    11041 atgtagaggg taaaatatat acatatgact ctaacttgct ttgtatggga actggtttac
    11101 ttgtaataaa agcaggagaa cttgcaagtg aaggaaagac tgttgatgaa atattgcctg
    11161 ttttggatga attaaaagag aaggcttttg ttatattttc agtagataca ctggaatact
    11221 tacaaaaggg aggtagaata tcatctacta aggcagcaat aggaagtata ttaaatataa
    11281 aaccgattct tgaaataaga gatggactag tttcgcaaat atctcaagta agagggaaaa
    11341 agaatgtaat ttctaagatg atggaattga taaaatcaaa ttgtggggat gatttatcag
    11401 accaaattgt gtatatagga tatagtgatg attttaagga aaaagaacaa ttatctaagg
    11461 ttatggaaga tgaattaaga cctaaagaaa ttagattttt ccaaattggg gtgtgtatag
    11521 gtgcacatgc tggtcctggt gtcactggta tcatatgttt aaaaaataaa taaatatatt
    11581 ttgaggtaat attatttatt tttttattaa attaggatag aatactaaat attgagatgc
    11641 taaattggat ttattttgga taaaattaag tgttgattta ttagatttag tttgtaggga
    11701 agtgtgtaaa tagataagag tctattgcac acttttttat agtaatattt attaaaaaat
    11761 tttcaaaatg tacttgtaaa ttgtatataa aaaaattata aataggatgt aaaagtaaat
    11821 aagagtaaaa atatcataaa tgtatgagga gtgaaattta atgcgtttca aaagaatatt
    11881 gatatttata ttaactgtag caatgacagc tggaatgttg gttggatgtg atagtaatca
    11941 aagtaataat cctagttcta gcaaagataa taaaagtgat ataagcgaca aaagtaattc
    12001 aaaaaaatct gtatctatga tactagatat tgaaggaaca aataatgaag ctatgaataa
    12061 ttcagcttta ttagcattaa acaatgctca aaagaaactt aatatagaca caaacaaagt
    12121 ggaatcagat gattcatcaa ctttttctaa tagtatagat attttatgta atgacaatta
    12181 cgatttaatt atagctgtag gagctagatt tgcaaaacct ttggagatgg tagccaagaa
    12241 atatccaaag caacaatttg caattataga ctatgagtat gacaagcaac cttctaatat
    12301 aacttcaatt tcttatgaag ataataaatc tggataccta gctggattaa ttgctggaaa
    12361 gatgactgaa agcgataagg ttggttttat aggtggaaca aagagttcga gtagagacaa
    12421 atttgaaaca ggttttaaag aaggtgttaa gttttctaat agtagcataa aagatatttc
    12481 agttgaatat gcagatgttt ttaaagatag aaaatcagta gaatctatag ctaaaaagat
    12541 gatggataat ggagtggata ttattttttc tactactgaa gatgattcaa aagctgtaat
    12601 aggtgcagta agaacaaaaa ataaaaaagt tatagcaaca aataaagacc aacatgagtt
    12661 agctccagaa aatgttatca gttctattgt aaaagatttt gagaattcta cttataattt
    12721 gatacaaagc tttgtaaaag gaaattataa aggtggaaaa gttatagaaa atacagttaa
    12781 gtctggaagt actgggcttg cccaaaatag ttctcaaaat attcctccag atgtgttaga
    12841 atatgttagt aagaacaata aataatcttc tataatatcc aggaatactt atgactttag
    12901 ttatgagttg aactggctgt tataatagac atatatagaa aggcatatgt agaggtaggt
    12961 aatgcctatc taaatcaggg agtgaatttt gtgaaaataa aaaggatatt gatggtttgc
    13021 tttgtggtag tttttacaat ttttatatta gctatatgta attttgattt tgggtttgat
    13081 aaagtaaata agaataattc taatatagat agtgctaaat caaaacagag tgagtctaaa
    13141 aatgtaccga ctttagcaga aaaggataaa tcaaatagta aaagtaagaa taaagaagat
    13201 gagacagaag atgatttata tgtaagaaaa actgttggag tattattaga tactgaaaac
    13261 ttaaataatg aatcatataa taataatgta ctatcagcct taaaaagggc tgacgaagat
    13321 tttgattaaa aaatagctac gcacactcgt gactttagtc atgagttagt agctaaaata
    13381 gtcagcatat agggaaactt atatgtagag ataggataga acctatccaa tactacttga
    13441 attgctg
//
LOCUS       DAFNNB010000108        13228 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0109, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000108 DAFNNB010000000
VERSION     DAFNNB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 13228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13228
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0109"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            296..1177
                     /locus_tag="L3M60_002547"
     CDS             296..1177
                     /locus_tag="L3M60_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6227200.1"
                     /translation="MNLYHLRYFVTLAHLEHYTKAAENLSITQPSLSHAISLLENELG
                     VALFEKEGRNIVLTKYGKIFLKDVEKSLEILDSSVKSLKITGTGEGQIDLAFLRTLGT
                     DFIPDIVHKFLKSNPTKSIDFKFHTGVTTDIIQGLKERKYDIAFCSKLEKEKGIEFIP
                     VAKQDLVLIVPYSHPLAAKDTIDLKETIPYPQIVFNQRSGLRYIIDDMFKKINQQPNI
                     VYEVEEDQVIAGLVAKNFGIAVVPNMNMLSFTKVKVIQIIHPSWERNFYLAFIKDRYF
                     PPAIKNFKSFVIKNAQL"
     gene            complement(1385..2248)
                     /locus_tag="L3M60_002548"
     CDS             complement(1385..2248)
                     /locus_tag="L3M60_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HBN6227201.1"
                     /translation="MVSSYVKRVSKIIKKKYIMFIDESGRADINHEDPFTLTGVIFEY
                     KYAVSQGDYVCSLRKEMDAFKEYCFGTSNIGIHLTDISRKSRAFEKFDDSQIKLFYDE
                     LPLFLSRLECTIISVTVDKARLKKYYAPSKEPYVVAFIHVLQNFYSFVNNETVESARI
                     VIESRDDVSNLKVQKAFFDVFNNGTIYLDIDEELRDKVKGFIIAKKEDADYREGLEIA
                     DILCNPLSRARRGLIEANPKCMQYGEKNKIFKSVRNKIYTPTSVNDIRNWGFKEVPII
                     EPVGPWLEKMR"
     gene            complement(2403..4337)
                     /locus_tag="L3M60_002549"
     CDS             complement(2403..4337)
                     /locus_tag="L3M60_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp22"
                     /protein_id="HBN6227202.1"
                     /translation="MKKSLKQYLVLALTLVLVFACGVPESSAASKHVIIVNSRKNTLG
                     YFVNNKLVKEFRVATGKKGSETPTGKTKVVNKIKNRPYYKGNIPGGSPRNPLGDRWMG
                     LALKGTYGDTYGIHGNNNESSIGKHISGGCIRMHNKDVRWLFDQVPVGSDVIIDYSND
                     SYVKIAAKYKINLNQTGWKTENGKKYYVKSDGTYQKNSWLKVNGKMYYFDASGVMQTG
                     WKTINNKKYYLGTDGARVSGWKIIDGKTYYFNSDGVMQTGWQEKNGKKYYLGADGLSV
                     TGWQEIDGNKYYFDKTGIMQIGWQQIEGKSYYLDKDGKMLTGAQKIDGKDYTFNEDGT
                     INPTWDTIIGANRFDTAKKISSVGNWNADSSDTVILVNGNAIADGITATPLASSYDST
                     ILLTNTDNLPNETVEEMKLLAPKTVILIGGENAISSKLEQEIKTIFNAETKRIAGQDR
                     YQTATRIAEELGSREEIKTAYIVSGNGEADALSVASKAGEEKQPIILVNKDGITEESY
                     KWLTERKLENAYFIGGPSAISDSVIARMNDITTEDISGNRIYGDSRVDTNAKVIEKFY
                     GDADLQAVLVSKSDALVDALSAGPLAVKLHSPIVLMYNSGLSSEQQRVFANKKVETPY
                     QIGGGVSYIVMDKLMDILGK"
     gene            complement(4449..5462)
                     /gene="galE"
                     /locus_tag="L3M60_002550"
     CDS             complement(4449..5462)
                     /gene="galE"
                     /locus_tag="L3M60_002550"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBN6227203.1"
                     /translation="MAVLVAGGAGYIGSHTAIELLESGYEVVIVDNLSNSNLIVVDRI
                     KELSKKPVKFYNIDIRNKDEMHIVFKENNIESIIHFAALKAVGESVEKPIEYYSNNLI
                     STLNLFELMREYGVKKFVFSSSATVYGDPHTCPILEDFPLSVTNPYGRTKLMIEQMLV
                     DISKADKSLDIALLRYFNPVGAHKSGRIGEEPNGVPSNLMPYITKIAVGKLKELSVYG
                     NDYPTHDGTGVRDYIHVLDLATGHVKALQKLEENPGLVVYNLGTGKGYSVLDLVKAFS
                     KASGKEIPYKIVGRRAGDVAMCYADSSKAEKELGWKAKYELEEMCEDSWRWQSMNPNG
                     YEE"
     gene            complement(5486..6355)
                     /gene="galU"
                     /locus_tag="L3M60_002551"
     CDS             complement(5486..6355)
                     /gene="galU"
                     /locus_tag="L3M60_002551"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014466379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBN6227204.1"
                     /translation="MTVKKAIIPAAGLGTRFLPATKSQPKEMLPIVDKPTLQYIIEEA
                     IESGIEEILIITGRNKKSIEDHFDKSVELELELEQKGKTEMLEMVRDISNMVNIHYIR
                     QKEPKGLGHAIHCAKSFIGDEPFAVLLGDDIVDSEVPCLKQLIDTYNEYKTTVLGVQK
                     IAKEDTNKYGILDVKHIEDRVYKVKDMVEKPAIEEAPSDIAILGRYIITPAIFNILEK
                     QTPGKGGEIQLTDALQTLGKQEAIYAYNFEGRRYDVGDKIGFLEATIDFALKRENLKD
                     DLMDYMRKKVSEK"
     gene            complement(6553..6873)
                     /locus_tag="L3M60_002552"
     CDS             complement(6553..6873)
                     /locus_tag="L3M60_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227205.1"
                     /translation="MSSKYNVWNFNYEFLGLDSGDEKNNEVYYEIDLDEILNENYIDE
                     DIDSDLDSDGNSILGELISEMDIDEDVSVDMTYQELEVDLEDLDSYIDEDIDSDIKGI
                     LDEI"
     gene            complement(6863..8854)
                     /locus_tag="L3M60_002553"
     CDS             complement(6863..8854)
                     /locus_tag="L3M60_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227206.1"
                     /translation="MLKLGEKIIYELSDGFELSGISRYSKEYKKLHITNLGNIIISKS
                     DEVNKDTGVKYIYSFSDHKEKLEAVLEDSQIIVYGNNLPFYIKSENSSKNVTANLTMK
                     ITLDEYDLICKSQREFIFYLKDNMTILSLDDNKLYMGGINKDNEKFIFISGKNRFEVN
                     FDDIERYILEDKRVSLKGYFHMEREGIIVRSVSIFNNNIDSVVPSDLNERVKDNQKIG
                     NLPKDCDIVFCKISGNIDGFDYKNTNMLLVKYQDQLIFINKKSKKTIVKSAKDNCSKL
                     NLGEDIILYDNKNVFNLHINDKNREIMQIDDLNDIENETVGYTLKHAPFFIQEDFDSL
                     TILKSFQKEIISIKNSDIKDIVINKELENENSNFVETEIKFNNQKILLNLSKNMVQKL
                     MQDVFIYAKQPLLKENSIEVIYKNWSKAMNDMIIFNFFGNIYYMKSEFDKILEKELND
                     EIRIEVINSLYKQIQEHRNNLDLLSAYMPRILENQEIDLFEKYNTKLDVQVFKQIKNL
                     LSDLSYNISSYLNEVEKSLDNIIFVISGEDKKKYNYRMLKESESASLDVFLKQAISRL
                     NHLVENMYPYYVDETSREMFKLFELLWKNYRNIDDDSIKEILFERITNTYVFKQLTLN
                     NSTKERRKDIIEKIYNSVDYGTNKLDENMFFTGGIKYVK"
     gene            complement(8975..11509)
                     /locus_tag="L3M60_002554"
     CDS             complement(8975..11509)
                     /locus_tag="L3M60_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HBN6227207.1"
                     /translation="MKVLSENNIIRNTVLNVTASFLKQESKINEKLDGVLEKKFEKVE
                     FNEAKYAELLKFNILFYKTLARNTEPLIGKWIVDKYIPEIDELEKDLELTTAKCRKYV
                     NKAMKEGLDSLKANDLNSFLAYDKMELSEKRRRLEKDYKVLNLYKDLLNISLRKISLE
                     KKECGDLFLKNQADAKRELKREIIFCVNKILASNKDVVSNNVEGNNEQDRLEIKATEI
                     EDLQLGKDNSENDMLLNEIKNSKVKHESKLSDVDSKFIDKLNSLNNESISQTIHNEYK
                     KLCGLEELHSVEGYGFDKEIIKDFACATVVLEFLKRRNRDLIEGAMRLTIIGEFGPEN
                     FKEFVDYVIKNKTEISEDTWNKAYNLIKDDYSELENHEIALKRTRKNKDIDIEEYIYM
                     IKNADKDICFRSSISIEDDVEEETKEEVASNNQDIDNFVEDKDITDKEYDSNKEDIIE
                     SENKKSKKKAKLFGFIKKDNEEVEQEEENLNGISSDVILDEPIENNRVKSEEIEQNEL
                     KEIKQEEPSQHIEEERSVKIEKPINNNLDEKVSLNNESKLEKESRNLEDEKVKEEKLE
                     NEKSVVIPINKKENSNKKSKNSSKDKSREHRKDAKNYVSDKEDSLDDEDVVSKKNRLK
                     ETIIAVVIVVIVGVGYFITVGNNKKNDKEDIPKSSTQQQANNKLTEEEKKAQAEKEKK
                     EAEEKAKAEKEAQEKAQAEKKAKEMEAYKDGKGIYYTVYAGSLKVEKTAKETAKEYEA
                     KGISSTIIQENGYYKIKIGDYSQYGEAQEKCNELAKKSIDTYIAMYDKYYDYKLEELK
                     ESAPSLSAEELKQKYEDLRSELKNKSGYREYVKHLDKLYEEIVEGA"
     gene            complement(11787..12121)
                     /gene="rnpB"
                     /locus_tag="L3M60_002555"
     ncRNA           complement(11787..12121)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="L3M60_002555"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12253..12930
                     /gene="srtB"
                     /locus_tag="L3M60_002556"
     CDS             12253..12930
                     /gene="srtB"
                     /locus_tag="L3M60_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtB"
                     /protein_id="HBN6227208.1"
                     /translation="MKKLYRIIINIILILVILYSGFNIYSKLSKYNHDTKTSSELQKK
                     EYKKEDLSKINSDFKFWLSVENTNINYPVVQSKDNSYYLDKDFYKKDSISGTLFMDYR
                     NKSLDDKNVIIYGHNMKNKTMFNNLNKFKDADFFRQNNKIKINLNGKEFLYDVFSAYI
                     VESDYDYLKTNFNSESDYQNYINDITSKSLYKSPIKVDINDKIITLSTCTYEFDDARM
                     VIHGRLI"
     gene            complement(13078..13152)
                     /locus_tag="L3M60_002557"
     tRNA            complement(13078..13152)
                     /locus_tag="L3M60_002557"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13118..13120),aa:Thr,seq:tgt)
BASE COUNT         4113 a         2308 c         1323 g         5484 t
ORIGIN      
        1 tttttatcta gattttataa atttattgta tcatgagact ttactaataa caaattgata
       61 attcactatt aattcataga ttttgtttat agattttatt tataagataa ttcttgtatt
      121 tttctactat gtcgtataaa tatctttatc ttataattca gacaaaaacg ataaattttt
      181 ttaaaagctt ttattcttac tacataatca tatataataa aaatgtaaaa aatatcttaa
      241 agttcaactt ctacaaatat ataaagaaaa atatctatga aaggatgaac aaaaattgaa
      301 tttatatcac ttacgctatt ttgttacatt agcacattta gaacactata caaaagcagc
      361 agaaaattta tctataacac aaccaagctt aagtcatgct atttctttac tagaaaatga
      421 acttggagta gctttatttg aaaaggaagg tagaaatata gtccttacta agtatggaaa
      481 aatattttta aaagatgtgg aaaaatcgct agaaatttta gattcaagtg taaaatcatt
      541 aaaaataacc ggtactggtg aaggacaaat agacttagct tttcttagaa cacttggtac
      601 tgactttatc ccagatatag tgcataaatt tttaaaatct aatcctacta aatcaataga
      661 ttttaaattt catactggtg taactactga cattattcaa ggtcttaaag aaagaaaata
      721 tgatattgca ttttgttcga aacttgaaaa agaaaaggga attgaattta tacctgttgc
      781 caaacaagac ttagtactaa ttgtaccata tagtcatcca ctagctgcaa aggatactat
      841 agatttaaaa gaaaccatac cttatcctca aatagtattt aatcagagaa gtggtcttag
      901 atatataatt gatgatatgt ttaaaaaaat aaatcaacag cctaacatag tctatgaagt
      961 ggaagaagac caagtaattg ctggattagt tgcaaaaaat ttcggaattg ctgtagtccc
     1021 aaatatgaat atgcttagct ttacaaaagt aaaagtaatt cagattattc acccaagttg
     1081 ggaaagaaac ttttacttgg catttataaa agatagatat ttccctcctg caattaaaaa
     1141 ctttaaaagc tttgtcatta aaaacgcgca actataattt attaataaag aggttatttt
     1201 taaatatcct cttttattct ttttataaaa taaaaaaatc tatactttta aaagtataga
     1261 ttcctaactg acagtcggta tccgagtctc agtaattatc gttatacaac agcaactgga
     1321 ttcagttcct gaataactat gttattttta ttatatcaga ttttttaaaa cttgtcaatt
     1381 gtatctaacg cattttttca agccatggtc caacaggctc tataatagga acttctttaa
     1441 aaccccaatt tcttatatca ttcacactcg ttggggtata tattttattt ctgacagatt
     1501 tgaatatttt atttttttct ccatattgca tacactttgg attagcttct attagccctc
     1561 ttcttgctct gctaagagga ttacataaaa tatctgctat ttctaaacct tctctatagt
     1621 ctgcatcttc ttttttggca attataaaac ctttaacctt atctcttaat tcttcatcta
     1681 tatctaagta tattgttcca ttattaaata catcaaaaaa cgccttttgt acttttaagt
     1741 ttgagacatc atcccttgac tctataacta ttcgtgcaga ttctacagtt tcattattta
     1801 caaacgaata aaaattctga agaacatgta taaaagccac aacatacggt tcctttgaag
     1861 gtgcataata tttttttaat ctagctttat ctactgttac tgatattata gtacattcta
     1921 gtctagatag aaataaaggc aactcatcat aaaataactt aatctgtgaa tcatcaaact
     1981 tctcaaaggc tcttgatttt cttgatatat ctgtcaaatg tattcctatg ttgctagttc
     2041 caaaacaata ttctttaaat gcgtccattt ctttcctcaa agaacataca taatcgcctt
     2101 gacttactgc atatttatat tcaaaaatta ctcctgtcaa tgtaaatggg tcttcatgat
     2161 ttatatcagc tcttccactt tcatcaataa acattatata ttttttcttt ataatcttac
     2221 tcaccctctt aacatatgat gataccatct atattcattt tatatgagtt ttaaaaatat
     2281 tccaagattt ttatataact taaacttaat tttatactaa aaaattgaga tgtaaaacaa
     2341 tcaagctttt attgattgaa tttcacatct caattacaat tatttttcag tatatatttt
     2401 cactattttc caagaatatc cattaattta tccattacta tataagatac tccgcctcct
     2461 atttgataag gagtttcaac ttttttattt gcaaatactc tttgttgctc tgacgataat
     2521 cctgaattat acattaaaac tattggtgaa tgtaatttta cagctaatgg tccagctgat
     2581 aaagcatcta ctaaagcatc tgatttacta actaatacag cctgtaaatc agcatctcca
     2641 tagaattttt ctattacttt tgcattagta tctactctac tatctccata tattctattt
     2701 cctgaaatat cttcagtagt tatatcattc attctggcta ttactgaatc gctaattgca
     2761 cttggcccac ctataaagta tgcattttct aattttcttt ctgttagcca tttataagat
     2821 tcttctgtaa ttccatcttt attgactaat attataggtt gtttttcttc tccagcttta
     2881 gaagctacag ataaagcatc tgcttcccca tttccagaaa ctatatatgc agtttttatt
     2941 tcttctcgac ttccaagttc ttcagctatt cttgtagcag tttgatatct atcttgacct
     3001 gctattctct ttgtttctgc attgaaaata gtttttattt cttgctctaa tttagaactt
     3061 attgcatttt ctccacctat caaaattact gttttaggtg ctaataactt catttcttca
     3121 acagtttcat ttggtaaatt atctgtatta gtaagtagta ttgttgagtc atagctactt
     3181 gccaaaggag ttgctgttat accatcagct attgcattac catttactaa tattacagta
     3241 tctgaactat cagcattcca gtttccaact gaacttatct tcttagctgt atcaaatcta
     3301 tttgcaccta taattgtatc ccatgtagga tttatagttc catcttcatt aaaagtataa
     3361 tcttttccat ctattttttg agcaccagtt agcatctttc catctttgtc taaatagtaa
     3421 gacttacctt ctatttgttg ccatcctatc tgcattattc ctgttttgtc aaagtaatac
     3481 ttattaccat ctatttcttg ccatcctgtt actgataaac catcagctcc tagatagtat
     3541 ttctttccat tcttttcttg ccatcctgtt tgcattactc catctgaatt aaagtaataa
     3601 gtctttccat ctattatttt ccatccagat actcttgctc cgtcagttcc taaataatat
     3661 tttttgttat taattgtttt ccatcctgtt tgcattactc ctgaagcatc aaagtaatac
     3721 attttcccat ttacttttaa ccaactattc ttttggtatg ttccatctga ttttacataa
     3781 tactttttac cgttttctgt tttccatcct gtttggttta aatttatctt atattttgct
     3841 gctatcttta cataactgtc attactataa tctataatta catctgaacc tacaggaact
     3901 tggtcaaata accatctaac atctttattg tgcattctta tacatccacc agaaatatgc
     3961 tttcctatag aactctcatt attatttcca tgtattccat atgtatctcc ataagttcct
     4021 tttaatgcta atcccatcca tctatctcct aatggatttc ttggacttcc tccaggtata
     4081 ttacctttat aatatggtct gttttttatc ttgttaacta cttttgtttt ccctgttggt
     4141 gtttctgacc cttttttacc tgttgcaact ctaaattctt ttacaagttt attatttaca
     4201 aaatatccta gagtgttctt tcttgaattt actataatca catgtttact ggctgcactt
     4261 gattctggta cgccacatgc aaatactaaa actaaagtca gtgctagtac taaatactgt
     4321 tttagacttt ttttcactac aacttccccc tcttttagaa acattactac atgtgtctaa
     4381 ttataacata aaattaccaa taccttatat ttacaaattt gttacattta tacatataag
     4441 catacattct attcctcata cccattagga ttcatgcttt gccatctcca tgaatcttca
     4501 cacatttctt caagttcata cttagctttc catccaagct ctttttctgc ctttgaagaa
     4561 tctgcataac acatggctac atctccagcc cttcttccta caatcttata tggtatttct
     4621 tttccacttg ctttagaaaa tgcttttact aaatcaagaa cactatatcc ctttccagtt
     4681 cctaaattat aaacaacaag tcctggattt tcttctaatt tttgtaatgc ttttacatga
     4741 ccagtagcta aatctaatac atgtatatag tctctaactc cagttccatc atgtgttgga
     4801 tagtcattac cataaacact taattccttc aatttaccta ctgctatttt tgttatataa
     4861 ggcattagat tacttggaac tccatttggt tcttcaccta ttctaccact tttatgagca
     4921 cccactggat taaagtatct taaaagagct atatccaatg acttatctgc ttttgatata
     4981 tctaccaaca tctgctctat cataagttta gttcttccat atggattggt tacagaaagt
     5041 ggaaaatcct ctaatatagg acaagtgtgt gggtctccat atacagtagc tgaagaacta
     5101 aatacaaact ttttaacacc atattctctc attaattcaa ataaatttaa ggtacttatt
     5161 agattattgc tgtaatattc aattggtttt tctactgatt cgccaactgc ttttaaagct
     5221 gcaaagtgta taattgattc aatattattt tctttaaata caatatgcat ttcatcttta
     5281 tttctgatat caatattata aaattttact ggtttttttg ataattcctt gattctatca
     5341 acaactatta agttactatt acttaagtta tcaactataa caacttcata acctgattct
     5401 aaaagttcta ttgctgtatg gcttcctata taccctgctc ctccagctac taaaactgcc
     5461 attgtaatta cctcttttct aatatttatt tttcacttac tttttttctc atatagtcca
     5521 ttaaatcatc ttttaaattt tctctcttta gagcaaagtc tattgttgcc tctaaaaaac
     5581 caatcttatc tcccacatca tatcttctac cttcaaagtt ataagcataa atagcctctt
     5641 gttttccaag agtttgaagt gcatctgtaa gttgtatttc tccaccttta ccaggtgttt
     5701 gtttctctaa tatgttaaat attgcaggag ttattatata tcttccaagt atagctatat
     5761 cagatggagc ttcttcaatt gcaggttttt caaccatatc tttaacttta tataccctat
     5821 cttctatatg ttttacatcc aatataccat atttattagt atcttcttta gctatctttt
     5881 gaacacctaa tacagttgtt ttatactcat tataagtatc tattaattgt tttaagcatg
     5941 gaacctctga atctactata tcatcaccta ataatacagc aaaaggttca tcacctataa
     6001 aactttttgc acagtgaatt gcatgtccaa gaccttttgg ctctttttgt cttatataat
     6061 gaatgtttac catatttgat atatctctta ccatttctaa catctctgtc ttaccttttt
     6121 gctctagttc taactctaat tcaactgatt tatcaaaatg atcttctata gattttttat
     6181 ttctacctgt tattataaga atttcttcaa ttcctgattc tatagcttct tctattatat
     6241 actgtaaagt tggtttatct actataggaa gcatttcttt gggctgtgat tttgttgctg
     6301 gtaaaaatct agttcctagc cctgctgctg gtattatcgc ttttttaact gtcatctgca
     6361 ttttttcacc cttcttcgta aattttctat tgtaccaaat taatatacca tatttatact
     6421 ttttcttcaa actctatatt atcccaatat ttaatgctat aagataatat aaggtagatg
     6481 aaatttcatc tacctttagt tttataagtt aatattgtat tgtttatatt ttatctaaaa
     6541 cttatacagg tactaaattt catctaagat gccttttata tcactatcta tatcttcatc
     6601 tatataagaa tctaaatctt ctaagtcaac ttctagttct tgatatgtca tatcaacact
     6661 gacatcttca tctatatcca tctctgatat taattctcct aaaatactat ttccatcaga
     6721 atctaaatca ctatctatat cttcatctat atagttttca tttaaaattt catctaaatc
     6781 aatctcataa taaacttcat tatttttttc atctcctgag tccaagccta agaactcata
     6841 gttaaaattc caaacattgt atttacttga catattttat tcctcctgtg aaaaacatat
     6901 tttcatctag tttattggtt ccataatcaa cagaattata tattttttca attatgtctt
     6961 ttcttctttc ttttgtacta ttatttaatg tcaattgttt aaatacatat gtatttgtta
     7021 ttctctcgaa taatatttct tttatactat catcatcaat atttctatag tttttccaca
     7081 ataactcaaa taacttaaac atttccctac tagtttcatc tacataataa ggatacatat
     7141 tttctactaa atgatttaat cttgatatag cttgttttaa aaatacatca agacttgcac
     7201 tttcactttc ttttaacatt ctatagttgt atttcttctt atcttctcct gaaattacaa
     7261 agatgatatt gtctaagctt ttttcaactt catttaaata actacttata ttatatgata
     7321 agtcagacaa tagattttta atctgcttaa acacttgtac atcaagttta gtattatact
     7381 tttcaaacaa atctatttct tgattttcta atattcttgg catatatgct gacaataaat
     7441 ctaaattgtt tctgtgttct tgtatttgct tataaagtga atttataact tctattctta
     7501 tttcatcatt taattctttt tctaaaatct tatcaaattc agatttcata taataaatat
     7561 ttccaaagaa attaaatatt atcatatcat tcatagcttt agaccaattc ttatatatca
     7621 cttcaataga attttcttta agtagaggtt gctttgcata tataaataca tcttgcataa
     7681 gtttttgaac catattttta cttaagttta aaagtatttt ttgattatta aattttattt
     7741 ctgtttctac aaaattagaa ttttcgtttt caagctcttt attaattaca atgtctttta
     7801 tatcactatt ttttatagat atgatttctt tttgaaaaga ctttagaata gtcaagctat
     7861 cgaaatcttc ttgaataaaa aatggagcat gttttaatgt atatcccact gtttcatttt
     7921 ctatatcatt taaatcatct atttgcataa tttctctatt tttatcattt atatgtaggt
     7981 taaatacatt tttattatca tataagatta tatcttctcc caaatttaac tttgaacaat
     8041 tatcttttgc tgactttaca atagtctttt tagatttctt atttataaaa attaattggt
     8101 cttgatattt tactaaaagc atgtttgtgt ttttataatc aaatccatct atatttcctg
     8161 atatcttgca gaacactata tcacaatctt taggaaggtt ccctattttt tgattatctt
     8221 taactctctc atttaaatca gatggaacta cactatctat attgttatta aatatagata
     8281 cagaccttac tataatcccc tctctttcca tatgaaaata tcccttcaag gacactcttt
     8341 tatcttctaa aatatacctt tctatatcat caaaatttac ttcaaatcta tttttcccac
     8401 tgataaatat aaacttctca ttatctttat taattccacc catataaagc ttattgtcat
     8461 ctaaacttaa tatagtcata ttatccttta gataaaatat aaactctctt tggcttttgc
     8521 aaattaaatc atattcatcc aaagttattt tcatagttaa atttgctgta acatttttac
     8581 ttgaattttc actttttatg taaaaaggca agttatttcc atagacaatt atttgagaat
     8641 cttctaaaac tgcctctaat ttttccttat ggtctgaaaa actatatata tattttacac
     8701 ctgtatcctt attaacttca tctgatttgc tgattattat attccctaga tttgttatat
     8761 gaagcttttt atattctttt gagtatctac tgataccact aagctcaaat ccatctgaaa
     8821 gctcatatat gattttttct cctaatttca acataccttc accacctaaa cagaattata
     8881 agtattttat aaaggtgaac aaaatgtctc aaaataaata atattttttt atacttttta
     8941 ttctgagaca tagtttctcc ttttttataa tactctaagc accttcaact atttcttcat
     9001 aaagtttatc taaatgttta acatattcac gatatccaga tttattcttt aactcacttc
     9061 ttaaatcttc atatttttgt ttaagttctt cagcactcaa agaaggagct gattctttta
     9121 attcttctaa tttataatca tagtacttat catacatagc tatatatgta tcaatagact
     9181 ttttagctag ttcattacat ttttcttgtg cttctccata ttgagaataa tcacctattt
     9241 ttattttata gtatccattt tcttgaatta ttgttgaaga aataccttta gcctcatatt
     9301 cttttgctgt ttcttttgct gttttttcaa ctttaagtga acctgcataa acagtgtaat
     9361 atataccttt accatcttta tatgcttcca tttcctttgc tttcttttca gcttgtgctt
     9421 tttcttgagc ctctttttca gcttttgctt tttcttctgc ttcttttttc tctttttcag
     9481 cttgtgcttt tttctcttcc tcagttaatt tattgttggc ttgttgctga gttgatgatt
     9541 ttggaatatc ctctttatca tttttcttat tgttacctac tgttataaaa tatcctacac
     9601 caacaatcac tactataact actgctataa tagtttcttt taatctattc ttcttagaaa
     9661 caacatcttc atcatctaaa gagtcttctt tatctgatac ataatttttc gcgtctttcc
     9721 tatgttctct agatttgtcc ttagaagaat ttttagattt cttattacta ttttctttct
     9781 tattaatagg tattactact gatttttcat tttctaattt ttcttctttt actttttcat
     9841 cttccaagtt tctactttct ttttcaagct tagattcatt gtttaaagat accttttcat
     9901 ctaaattatt atttattggt ttttctattt ttacactacg ttcttcttct atatgttgac
     9961 ttggttcttc ttgttttatt tcttttagtt cattttgctc tatttcttct gattttactc
    10021 gattattttc aattggttca tctaaaatta catcagaaga aatgccattt aaattttctt
    10081 cttcttgctc aacttcttca ttatcttttt taataaatcc aaataattta gccttctttt
    10141 tactcttttt attttccgat tcaattatat cttctttatt gctatcatac tctttatctg
    10201 taatatcttt atcttctaca aaattgtcta tatcttgatt attactagct acttcttctt
    10261 tagtctcttc ttcaacatca tcttctattg aaatactact tctaaagcaa atatctttat
    10321 ctgcattttt tatcatgtaa atgtattctt caatatcgat atctttattt tttctagttc
    10381 tttttaatgc aatttcatga ttttctaatt cactatagtc atcttttatt aggttatatg
    10441 ctttattcca cgtatcttca ctaatttctg ttttattttt aattacatag tctacaaatt
    10501 ccttaaaatt ttcaggacca aattctccaa ttatagtcag tctcattgca ccttctataa
    10561 ggtctctatt tcttctcttt aaaaattcta atacaactgt tgcacaggca aaatccttaa
    10621 taatttcttt gtcaaaacca tatccttcaa cagaatgaag ttcttctaat ccacataact
    10681 tcttatattc attgtgtatt gtttgagata tactttcatt atttaagcta tttagcttat
    10741 ctataaactt cgaatctaca tcacttaatt ttgattcatg ttttactttt gaatttttaa
    10801 tttcatttaa tagcatatca ttttctgaat tatctttacc taattgcaaa tcctcaattt
    10861 cagttgcttt tatttctaat ctatcttgtt cattatttcc ttccacatta ttagacacta
    10921 catctttatt actagccaat attttgttta cacagaatat gatttctctt tttaattctc
    10981 ttttagcatc tgcttgattt ttaagaaata aatctccaca ttctttcttt tctaaactta
    11041 tttttctaag agatatattg agaaggtctt tgtataggtt taaaacctta taatcctttt
    11101 ctaaacgtcg tcttttctct gatagttcca ttttatcata agctaaaaaa ctatttaaat
    11161 cattggcttt tagagagtct aatccctctt tcatagcctt atttacatac tttctacatt
    11221 ttgctgtagt taattctaaa tccttttcta attcatctat ttcaggtatg tacttatcta
    11281 ctatccattt gccaatcagt ggttctgtat ttcttgctaa tgtcttataa aacaatatat
    11341 taaattttaa aagctctgca tattttgctt cattaaattc tactttttca aatttctttt
    11401 ccaaaacacc atctagcttt tcatttattt tagactcttg ttttaaaaaa cttgctgtga
    11461 catttaaaac agtgtttctt attatattat tttcactcaa aaccttcact ccttaaatca
    11521 attattttat tgtaatattt acattgtgca aagttgctaa tttgattatg atatttaaat
    11581 aattatttac taaatttgct acatcttata tcatatcata ttatttacat cattttactt
    11641 tttcttttta tatctatctt atattatacc atttttaata tctgtactaa cttatattac
    11701 aatattcctc aaaattttaa aagtagatta gacaaattta caaataaaaa aacatcagtt
    11761 taaactgatg tttttgatta tcaaaataat taagcaagtc tataagcgga gttctgtatt
    11821 agataatcat ctttctaggc ctattgtcac caataggctc aagcgaccta cctaccggag
    11881 agtacgagca gcactcttta ttcctatctt ggtcttgctc caggtggggt ttacatagcc
    11941 aactagtcgc ctagctgctg gtgagctctt acctcgcctt tccatcctta ccacaatgtt
    12001 ggcggtttat ttctgttgca ctatccttag gatcgctccc actaggcgtt acctagcacc
    12061 ctgctctatg gagctccgac tttcctcatg taaaattaca tgcgattatc tggcttactt
    12121 aatatttatt ttacttagtt atgttaacac aaattctttt acatgtcaaa gtaatattat
    12181 actaaaaatt taaatttatt caaaaatagt gtaatatagg tatataaact acatttaact
    12241 cgaggtgata tattgaaaaa gttatataga ataataatta acattatact aatattagta
    12301 atactttaca gtggatttaa tatatactca aaacttagca aatacaatca tgatactaaa
    12361 acatcctcag aacttcaaaa aaaagaatat aaaaaagagg acttatcaaa gattaacagt
    12421 gattttaaat tttggctttc tgttgagaat acaaatataa actatccagt tgtacaatct
    12481 aaagataatt cttactatct tgacaaagat ttttataaaa aagattctat ttcaggtaca
    12541 ctatttatgg attatagaaa taaatctctt gatgacaaaa atgtcataat atatggacat
    12601 aatatgaaaa ataaaactat gttcaacaac ctaaataagt ttaaagatgc tgatttcttt
    12661 agacaaaata ataaaataaa aataaactta aatggaaaag aatttttata tgatgttttt
    12721 tctgcttata ttgtagagtc tgattatgat tatctaaaaa caaattttaa cagtgaatct
    12781 gattatcaaa attacataaa cgatattact tctaaatcat tgtataaatc tcctattaaa
    12841 gtagatatta atgataaaat aattacactt tctacatgta cctatgaatt tgatgatgcc
    12901 agaatggtga ttcatggtag attgatttaa aactcacagg cacagtttaa aacaataatt
    12961 ttaaactgtg tcatcttttg ttccaataat ttatattagg ctaataaaaa tatgtagtac
    13021 aaaataaaag acacattaaa caataaaaaa agactatgct aaaaacatag tctttactgg
    13081 agctggtgat aggaatcgaa cctacaacct gctgattaca agtcagctgc tctaccgttg
    13141 agccacacca gcacacttat tctattataa attaataaag ctaactttta gaattaacac
    13201 tctaaaaact acatatatat tttaataa
//
LOCUS       DAFNNB010000109        12871 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0110, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000109 DAFNNB010000000
VERSION     DAFNNB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12871
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0110"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(48..656)
                     /locus_tag="L3M60_002558"
     CDS             complement(48..656)
                     /locus_tag="L3M60_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HBN6227209.1"
                     /translation="MEKDFFRKNGIDLAKSILGKYLIRKYENKVIVTKIIETEAYMGV
                     NDKGAHVYGNKKTDRTKPLYLDGGHIYVYLIYGMYNCLNLSANIENVPECVLIRGVEP
                     ITSLDEISMNRYNKAYTELSKYQVKNITNGPGKLCKALKIDRSLNSKSIMGEELYISN
                     FYYDDKGKKVFSKDKLDIKTSKRINIDYAEEAKDFLWRFYIE"
     gene            complement(765..3503)
                     /locus_tag="L3M60_002559"
     CDS             complement(765..3503)
                     /locus_tag="L3M60_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBN6227210.1"
                     /translation="MESKGTLSKNKVYILANGLFLVIALLNISLYTVSINMYRIILLW
                     IFGYSIVYISNFKTDEFINILKVGCLVLVFVSILGVVNLKIQKFQIVLLNLSIINMST
                     FIYLTISKVKKLRISPILIFYSAFVILDYFIRNIFRDINWNMIIVLINVCISLFNLLY
                     LLNKLDSNIEHYKLIKDLTYFIFFTTIIGFVAIYKINIVDSNRFIGVIYLLLCNHTYY
                     VYVYSLRNRVVYPYYELDSINKKLSKKSEQLGKINLVIEKDMIIQRKLKNYIDQRKEL
                     LRQALDTIPNVWIVTDYDFNISYTNNKFKDEFSKDMDNYYKILTFIKEDEKVAEKLKK
                     FDNDISIIDKLVELNGKIYLLSLSNNKDESNYLISLNDITNEIKIDEEIRHINKDYEN
                     IILNIPSPILVRSAEGGIKKNKVISINKKYEKSFKCTSSDLVDMTLEQYFETFNIDFF
                     DNREFKRLNLTQEKKAEIVSYNDTANCIVNFVMSDSEGEEHVEEVRVGDYWSDNKLFK
                     LLTFRDITKEINILRTVNEQKIIYEKLLDVIPEAIFLEDLSTSRVLYTNRAFRELFGI
                     SSDVLGVTTQKYRNILVKKYINNLNIGEREKSIHIVNENNHIKEVKMISRTLYFGQKR
                     SRVRIIKDLSVQRESERLKKALIKQRQYDQMKMEFYANISHELKTPLNNIYSSVQLVE
                     NLYKKGKIIDFQDILKEHIKITKQNMFRLLRLIDNIINISQVKSDIYKIKAVNFDIID
                     ITERIVTSISSYAKSKGIDLIFDTDEETVMVGLDPESIERIILNILSNSIKFTLEGGE
                     ILVGIYKKDETVEIIIKDTGVGIEKEKLNDIFNRFKQIENSGISNEFGSGIGLCLTKS
                     LIEIQNGKIYIDSKVGEGTNVKIIFPIKEVVEEVYDNSNYNDNIEKFEIEFFDIYK"
     gene            complement(3651..4442)
                     /locus_tag="L3M60_002560"
     CDS             complement(3651..4442)
                     /locus_tag="L3M60_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6227211.1"
                     /translation="MKLKKLLSVALVSAIAISAVGCSNKEDKKILVGASSNPHAKILE
                     VAKPLLKEKGYDLEVKIFDDYVLPNTALDEGSLDANFFQHIPFLEETVKEKGYKLTYT
                     SKVHIEPMGFYSEKVKALDEIKDGAVIAVPNDATNGARALKLLAKNKLIEVKDGELIT
                     KKDITKNPKNIQIKEMNAEQLPTVLKDVDGAVINSNYALTANLNPTKDAIVIESSDSP
                     YVNIIACRENNKDSDKIKALSEAMNSKEVKKFIQDEYKGSIVPAF"
     gene            complement(4479..5135)
                     /locus_tag="L3M60_002561"
     CDS             complement(4479..5135)
                     /locus_tag="L3M60_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227212.1"
                     /translation="MNSLIDFLTTLFPNALLQTLYMVIVPTIVATILGFILAIILVVT
                     KPDGLKPNSAINSALGFIVNIFRSFPFMILIVAMIPITRLIVGTSIGETAAIVPITIG
                     AAPFIARIIESSLNEVDKGLIEAAKSFGATKRQIVFKVMIKEAMPSIVSGITLSIISI
                     LGYTAMAGAVGAGGLGNIALIYGYQRFDTAVMVYTVIALVILVQIIQGVGNLAYKKLK
                     "
     gene            complement(5128..6093)
                     /locus_tag="L3M60_002562"
     CDS             complement(5128..6093)
                     /locus_tag="L3M60_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HBN6227213.1"
                     /translation="MISIKNVNKYYGKIQVLKDVSIEIESGEIFGIIGHSGAGKSTLL
                     RCINGLEEYQEGNVLVSDKEVKSLNEKQMRDLRKELGMIFQHFSLLERRTVFDNVALP
                     LECFGYSKAEIKKRVLELLEVVGISEKKNDKPRNLSGGQKQRVAIARALALNPQVLLC
                     DEATSALDPNTTKSILSLLEDINKKLGITIIVVTHQMEVIKQICGRVAIMENGEVLEV
                     GDTEEIFLRNTKGLRKLIGEESIILPKGTNIKILFPKDISNEAIITTMARELNIDVSI
                     IFGKLEQFKDDILGSLIINISDKSGEQVKQYLTSKGIRWEEMINE"
     regulatory      complement(6199..6305)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(6471..7049)
                     /locus_tag="L3M60_002563"
     CDS             complement(6471..7049)
                     /locus_tag="L3M60_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBN6227214.1"
                     /translation="MRRKFVFWTPDMHCSTPLLKELFNIVMNESTIKIEYSSKQKNSI
                     RTIVPIGLYAMNGLWYCPAYCIKSESIKEFRADRIVKILSIENLSNKKYNVLSSIHDY
                     FKNMEVGTDYRIKINLADEGVKHCETEFLLARGLKILSKGGYIDMYIPKSTLNWVAEY
                     FLTFGKNATIIEPIELKHLILFNIFLIMMFYS"
     gene            7205..7492
                     /locus_tag="L3M60_002564"
     CDS             7205..7492
                     /locus_tag="L3M60_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227215.1"
                     /translation="MNSCWERAVYCNPNGVLKRGVYVLTIKEKDSNNDKDSLVNRSNV
                     YRVNIRLKKETFTEMFGYIPKRPGVGQIVDMDFDFTKLDIVMPHPIYSWMG"
     gene            complement(7771..8370)
                     /locus_tag="L3M60_002565"
     CDS             complement(7771..8370)
                     /locus_tag="L3M60_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227216.1"
                     /translation="MKLKRTLFVGLALVLAIGITACTRNNEKPNENAGKNPNESVNNN
                     EGYMNYYSTSYNDYIAGLNRYSIYDTPESINNQFKDKEYPGNEKYLSDVKAAYKDSRD
                     KIQSFVTSLKKDGKTEDTELKKMNDDLITEGERLVKDIDKRIEKLDKVSDEDMKKSQN
                     DFIKLVHGTEDVGNDIGSGFRKMIKDMNDRLGITGNNKK"
     gene            8616..9212
                     /locus_tag="L3M60_002566"
     CDS             8616..9212
                     /locus_tag="L3M60_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3793 family protein"
                     /protein_id="HBN6227217.1"
                     /translation="MSCCNSQFCCKNKIESSYIKWLLEVLGPVFLGSKPAEIINISFT
                     DINREEKINDIYKYLYKCKKIDFIVIDKKEKGLKILFVNKKALSEKLKCKKTVNFLKF
                     LGYKQNTNVNAYLEHLVDKLKGDVFPDEIGIFLGYPLKDVIGFMGYSNYEVSMIKYWK
                     VYGDTKQSEDTYSKFLLHRKKMRKLLDYISVDKIVSCF"
     gene            complement(9309..10268)
                     /locus_tag="L3M60_002567"
     CDS             complement(9309..10268)
                     /locus_tag="L3M60_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6227218.1"
                     /translation="MTSLIMVLVGTVGCSTTNDKKDEPAKKEASLPKEINLTYVKSPL
                     NVPSILEKKDDLFGKEFKKDNIKVNFHELTTGPEQTQALAAGELDFLHALGGTSAIIA
                     ASNGVGLKITNIYSRSPKGYMLITNNENIKSIKDLKGKKIVGPKGTILHQLLIAALAK
                     EGYTIDDVEYVNMDIPSAASALESKSADVAMLAGPVALKTINSGAKMIVNGEGLVSGI
                     IVTAVSDDFAEKYPELVKRFMKVHEETLKYMNENKDKVMDVVSKEVGLSLDETKEMYS
                     WYDFSSKITDKDIKELEDTQEFLMSNGMQQKKINIKDMLYNQN"
     gene            complement(10302..11033)
                     /locus_tag="L3M60_002568"
     CDS             complement(10302..11033)
                     /locus_tag="L3M60_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227219.1"
                     /translation="MVRSGFLLENICKKYLVDNKEHLVLDNVSLNISSEEITVILGES
                     GCGKTTLLRILAGLENATTGNVYFFNNDKKCTPKVGMVFQESRLMPWLNVSENILLHT
                     EKDNRNKVDLDKYLKMMKLEKFKNSYPNELSGGMAHRVSIARALSFNPDILLMDEPFA
                     ALDYFTRRKMQKEVVSIHKNTKKGVVFVTHNIEEAMEIAKKIIVFSKNKRIKQFSVED
                     EYNRDLTKNYYINLKKEILRELGEF"
     gene            complement(11026..11796)
                     /locus_tag="L3M60_002569"
     CDS             complement(11026..11796)
                     /locus_tag="L3M60_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227220.1"
                     /translation="MFEKIHKKFKGYYIFFIILILWKIVCVLGIWSSYILPPPEIVLD
                     TFIKMIYDGSIFLNVYASVRRILIGFLISSLMAIPLGVVFGVNKKMYEYFKPLFEFMR
                     STPPLALVPMLILWFGIGEESKIIIIILASFFPIFLNTLKGIKNCDNKLIEVGKSFNL
                     SRKRIFYKIIIPNSMLDIVVGLSLGLGYSFRAIIGAELIAASSGLGYLISDGKDMSRT
                     DVVLVGIFVIGFLGILSDYLFSKIITKFSKGKQVDLNG"
     gene            complement(11881..12561)
                     /locus_tag="L3M60_002570"
     CDS             complement(11881..12561)
                     /locus_tag="L3M60_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIM24 family protein"
                     /protein_id="HBN6227221.1"
                     /translation="MCIARGSGKFFAKKGAMVAFKGNFNFEKLLLGPSNGGGLGRALL
                     GHVQRSLTGEQMPIMVVEGEGEIYLAQNAYHVDVISIDPGDSIYVESENLLAFSEQLN
                     YSVKLIGSGVISQKGLFTTHLANKTGQNQDVAIITDGNPLILEGPCCVDPDALVAWTG
                     REPYPKVSKLSWKTFIGQTSGESYHMEFVEPGQIVIIQPSERLSGLKTDSTPSDRMSS
                     GGLRIGID"
BASE COUNT         4213 a         2182 c         1297 g         5179 t
ORIGIN      
        1 tccataaaat accttcctat agatacatat cttacctata taaaaactta ttctatataa
       61 aaacgccata aaaaatcttt tgcttcttct gcataatcta tatttatacg tttactagtt
      121 tttatatcta atttatcttt actaaatact ttctttcctt tatcatcata ataaaaatta
      181 cttatatata attcttcacc cattatactc ttactattta gacttctatc tatttttaat
      241 gctttgcaaa gttttcctgg tccattagta atatttttta cttgatattt actcaactca
      301 gtgtaagctt tgttgtatct attcatagat atctcatcca aagaagttat aggctctacc
      361 cctcttatta atacacactc tggaacattt tctatatttg cagataaatt taagcaatta
      421 tacataccat agattaaata aacatatata tgtccaccat ctaaatataa cggcttcgtt
      481 ctatctgttt ttttattgcc atatacatgt gctcctttat catttacacc catatatgct
      541 tccgtctcta tgattttggt aacaattact ttattttcat atttccttat taggtattta
      601 cctaatattg attttgctaa atcaattcca tttttcctaa aaaaatcctt ttccaaaatt
      661 tcacctcata aaatttattt gcaacgcatc aaatacaact aaagtaataa ataaaattaa
      721 ctcttattaa tgtgatatat ataaacaata aattccgaca tttattattt atatatatca
      781 aaaaattcta tctcaaactt ttcaatatta tcattgtaat ttgaattatc atatacttct
      841 tcaacaacct ctttaattgg gaatataatt tttacattag ttccttctcc taccttgcta
      901 tctatataaa tctttccatt ttgaatttca atcaacgatt ttgtgaggca taaccctata
      961 ccacttccaa attcatttga tatgccactg ttttcaattt gtttaaatcg attaaatata
     1021 tcatttaatt tttctttctc tatacccact ccagtatctt ttataattat ctctactgtt
     1081 tcgtcttttt tatatatacc tacaaggatt tctcctcctt ctaaagtaaa ttttatagaa
     1141 tttgataata tatttagtat tattctttct atagattctg ggtcaagccc taccataaca
     1201 gtttcttcat ctgtatcaaa aattaaatct attcctttag acttagcata actactaata
     1261 gatgtaacta ttctttctgt tatgtcaata atatcaaaat taaccgcttt aatcttatat
     1321 atgtctgact taacttgaga aatatttatt atattatcta ttaatctcaa taatctaaac
     1381 atattttgtt ttgttatttt tatgtgttct tttaatatat cctgaaaatc aattattttt
     1441 ccttttttat aaagattttc aacaagttga actgaactgt atatattatt taaaggtgtc
     1501 ttaagttcat gagatatatt agcataaaac tccatcttca tttggtcata ctgtctttgt
     1561 tttattaatg ctttcttaag tctttcagac tctctttgca cacttaaatc tttaataatt
     1621 ctcactctac ttcttttttg tccaaagtat aatgttcttg atatcatttt tacttctttg
     1681 atgtgattat tttcatttac tatatggata ctcttctctc tttctcctat atttaaattg
     1741 tttatgtatt tttttactaa tatatttcta tatttttgtg ttgtaactcc tagtacatca
     1801 ctagaaattc caaacaactc tctgaaagct ctatttgtat ataatactct tgatgtactt
     1861 aaatcttcaa gaaaaatagc ctctggtata acatccagta atttttcgta tattattttt
     1921 tgttcattta ctgttctaag aatatttatc tctttagtta tatctctaaa tgtaagcaat
     1981 ttaaagagtt tattatctga ccaatagtct ccaactctaa cttcttcaac atgctcttca
     2041 ccttctgaat cagacatcac aaaattaact atacagtttg ctgtgtcatt gtaacttact
     2101 atttcagctt tcttttcttg tgttaaattt aatcttttaa attccctatt gtcaaagaaa
     2161 tctatgttaa aagtttcaaa atattgttct aatgtcatat cgactaaatc acttgaagta
     2221 catttaaaac tcttttcata ttttttattt atacttatta ccttattctt ctttataccg
     2281 ccttcagcac ttcttacaag tataggagat ggaatattta gtataatgtt ttcatagtct
     2341 ttatttatat gtcttatttc ttcatctatt ttgatttcat ttgtaatatc atttaaactt
     2401 attaagtaat tgctttcatc tttattatta cttaaactta acaaataaat tttaccattt
     2461 aactctacta atttatcaat tatacttata tcgttatcaa atttcttcaa tttttcagca
     2521 accttttcat cttcctttat aaaagttaat attttataat aattatccat atcctttgaa
     2581 aattcatctt taaatttatt atttgtgtaa cttatattaa agtcatagtc tgttactatc
     2641 catacatttg gtattgtgtc cagagcttgt ctaagtaatt cttttctctg gtctatatag
     2701 ttttttaatt ttctttgtat aatcatatcc ttttctatta ctaaatttat ctttcctaat
     2761 tgctctgact ttttacttaa ttttttattt attgaatcaa gttcgtaata tggatacact
     2821 accctatttc taagactata tacatataca taataagtat ggttacataa taataaatat
     2881 attactccta taaatctatt tgaatctact atattaatct tataaattgc tacaaagcct
     2941 ataatagttg taaaaaaaat aaaatatgtt aaatctttta ttaacttata gtgctcaatg
     3001 ttactatcaa gcttattcaa caaatatagt aaattaaaca aagatataca aacattaatt
     3061 aatacaataa tcatattcca gttaatatct ctaaaaatat ttcttataaa gtaatctaat
     3121 atcacaaaag cactataaaa aattaatatt gggcttattc ttagtttttt tactttagaa
     3181 atagttaagt atataaaagt agacatatta attatgctta aattgagcaa cacaatttgg
     3241 aatttttgaa tttttaaatt tactactcct aaaatactta caaaaactaa tacaagacac
     3301 cctactttca atatatttat aaattcatct gttttaaagt tacttatata aactatagaa
     3361 tacccaaata tccaaagtaa aattattcta tacatattta tagaaactgt atacaaactt
     3421 atatttaaaa gtgcaataac caaaaataac ccattagcta gtatatagac cttattctta
     3481 cttaaagttc ccttagattc catattcccc accacttctt catttttata tagttttacc
     3541 tataaatatg ttaccacaat tcttccaaaa aatttgtcac tttctagtta taaaaaaaaa
     3601 tgagtaaata tttttaattt tactcatttt gtagattttg tatattcaat ttaaaaagct
     3661 ggaactatac ttcctttgta ttcgtcttgt ataaattttt taacttcttt actattcata
     3721 gcctctgaca aagccttaat cttatcacta tctttattat tttctctaca agctattata
     3781 tttacatatg gagaatcact tgattctatt actattgcat ccttagtagg atttaaatta
     3841 gctgttaaag cataattaga atttattact gctccatcaa catcttttaa tactgttggt
     3901 aattgctctg cattcatttc ttttatttgt atatttttag ggtttttagt tatatctttt
     3961 ttagttataa gctcgccatc ttttacctct atcaatttat tttttgctaa taatttaagt
     4021 gctctagctc cattagttgc atcatttggc acagctataa cagctccatc ttttatttca
     4081 tctaatgctt taactttttc tgaatagaat cccattggct ctatatgaac tttagatgta
     4141 taagtaagtt tatatccttt ctccttcaca gtttcctcta aaaatggaat atgttgaaag
     4201 aagttagcat ctaaagaccc ttcatctaaa gctgtatttg gaagtacata atcatcaaat
     4261 atttttactt ctaaatcgta tcctttttct tttaacaacg gttttgctac ttccaatatc
     4321 tttgcatgtg gatttgatga agctcctact agtatttttt tatcctcttt gtttgagcaa
     4381 cctactgctg atattgctat tgctgacacc aatgctactg ataataattt ctttaatttc
     4441 attttttact ccccctcttt aaaatgttta taattaattt attttagttt cttataagcc
     4501 agatttccta ctccctggat tatctgaact aatattacaa gtgctattac agtatatacc
     4561 attactgctg tatcaaatct ttgatatcca taaataagtg ctatattacc tagtcctcca
     4621 gcccctactg cacctgccat agctgtataa ccaagtatag atattattga taatgtaatt
     4681 ccagatacta ttgaaggcat agcttccttt atcataactt taaatactat ttgtcttttt
     4741 gtagcaccaa aagattttgc tgcttctatc aatcctttat caacttcatt taatgagctt
     4801 tctataatcc tagcgataaa tggtgctgcc ccaatagtta taggcactat tgcagcagtt
     4861 tcccctatgc ttgttccaac gattaatcta gttattggaa tcattgcaac tataagtatc
     4921 ataaatggga aacttctaaa tatatttact ataaatccta atgcactatt tattgcgcta
     4981 tttggtttta atccatctgg ttttgtaact actaatatga ttgccaatat aaatcctaat
     5041 attgttgcca ctatagttgg tactataacc atatacaaag tttgtaacaa tgcatttgga
     5101 aatagtgttg ttaaaaagtc aattaaacta ttcatttatc atttcctccc atcttatgcc
     5161 cttactagtc agatattgtt ttacttgttc gccactttta tccgaaatat ttataataag
     5221 tgaacctaaa atatcatctt taaattgttc taatttccca aatattatag acacatctat
     5281 atttagttct cttgccatag tcgtaataat agcttcatta gaaatatctt tagggaatag
     5341 tatctttata tttgttcctt ttggtaatat tatactttct tctcctatta attttctaag
     5401 acctttagta tttcttaaga atatttcttc tgtatctcct acctctaata cttctccatt
     5461 ttccattatt gctactcttc cacaaatctg ctttatgacc tccatttgat gggttacaac
     5521 tattattgtt attcccaatt ttttatttat atcttcaagc aatgaaagaa ttgattttgt
     5581 agtatttggg tctaatgctg aagttgcttc atcacaaaga agtacctgtg gatttagtgc
     5641 taaagctcta gcaattgcaa ctctttgttt ttgaccacca cttagatttc taggtttatc
     5701 atttttcttt tcagatattc caaccacttc taataactct aaaactcttt ttttaatttc
     5761 tgcctttgaa tatccaaaac actctaatgg caatgctaca ttatcaaata cagttcttct
     5821 ttcaagcaat gaaaaatgtt gaaaaatcat tcctaactct tttcttaaat ctctcatttg
     5881 cttttcattc aatgatttta cttctttgtc agaaacaagt acattacctt cttggtattc
     5941 ctctaatccg tttatacatc taagtagtgt tgatttacct gcaccactat gacctattat
     6001 tccaaaaatt tctccagatt ctatttcaat actcacatct ttaagcactt gaatctttcc
     6061 ataatactta ttaacatttt ttatgctaat cataaaattt cctcccatat attctgctca
     6121 gtttttatgt ttatcctagc ttatgtatta caacaaaata aaagcccgag tataaactca
     6181 ggcttagaaa gctatttata ctcatctatc aactatatta gttgcaggat ttagcaccaa
     6241 tgtatacaaa atatactggt tgccgggttt caaagggcca gtccctccac cactcttgat
     6301 aagacgttat ttaatttaat tctattatca tttgttgcta tgttatatat aatatattca
     6361 ttaatagtat ttgtcaataa atttatttaa attttttatt ttttattatt gttaattaac
     6421 tatttttaat tagttttcta gctcctaaca ctttatattt taatgtctat ctaactatag
     6481 aacatcatta taagaaatat gttaaatagt atcaaatgtt taagttctat aggctctatt
     6541 attgtagcat ttttcccaaa ggttaaaaaa tattcagcca cccaattaag tgtagatttt
     6601 ggtatataca tatctatgta tccaccttta gacaaaattt tcaatcctct tgccaacaaa
     6661 aattctgttt cacaatgttt tactccctca tctgccaaat taattttaat acgataatct
     6721 gtgcctactt ccatattctt aaaataatca tgtatagaag ataaaacatt atatttctta
     6781 tttgataagt tttctatact caaaatcttc acaattctgt ctgcacgaaa ttccttaata
     6841 gattctgatt ttatgcagta agctggacaa taccaaagtc cattcatggc atacaaacca
     6901 atagggacaa tggtacgtat actatttttt tgctttgatg aatattcaat tttaattgta
     6961 gattcattca ttactatatt aaacaattct ttcaataatg gtgttgaaca atgcatatct
     7021 ggtgtccaaa aaacaaattt acgcctcata ttctcaatat tatattaaat atcatttggt
     7081 atacaattta atttttttta aaacagaaat agtttcttgc tcaaacggca aatcactata
     7141 agatactatt aaggctgatg atattatcaa atattgtcta tcaaacttag atgatgttgt
     7201 actaatgaat agttgttggg aacgagcagt ttactgcaat cctaatggtg ttttaaaacg
     7261 aggagtatat gttcttacca ttaaagaaaa agacagtaat aatgataaag attcgttggt
     7321 taatcgttca aatgtatacc gtgtaaatat acgattaaaa aaagaaactt ttactgaaat
     7381 gtttggatac attccaaagc gtccaggtgt aggtcaaata gttgatatgg attttgattt
     7441 tacaaaattg gatatagtca tgccacatcc tatctactca tggatgggat gaatatgcat
     7501 cctaagccct actgaaaaga catttaagaa ccttaaaact ttaataggag aatcttacaa
     7561 ttttgcaaaa gaaaaattca aaaagagaaa aaacaagtaa aaacatatga aaataacctc
     7621 taaataaatt ataataaaaa tattaaggtt acaagataaa aaattaatat cttaataata
     7681 tacaaaaaag cgaagccaaa aaatgtatta tttataaggc ttcgctatat ctcaaataat
     7741 ttatttcaaa ttttaaaact atttattcta ttatttctta ttatttcctg ttattcctaa
     7801 tctatcattc atatctttta tcatttttct aaaaccacta cctatatcat ttccaacatc
     7861 ttcagttcca tgaactaatt ttataaagtc attttgactt tttttcatat cttcatcaga
     7921 tactttatct aatttttcta ttctcttatc tatatctttt actaatcttt ccccttcagt
     7981 gattaggtca tcattcattt tctttaactc tgtatcttct gttttaccat cttttttaag
     8041 agatgttaca aatgattgta ttttatctct actatcttta tatgcagcct ttacatcact
     8101 tagatatttt tcatttcctg gatattcttt gtctttaaac tgattattta tactttctgg
     8161 agtatcataa atagagtatc tatttagacc tgctatataa tcattatagc ttgttgaata
     8221 ataattcata tatccttcat tattatttac agattcatta ggattttttc ctgcattttc
     8281 atttggtttt tcattatttc tagtacatgc agttatacct atagctaata ctaatgctaa
     8341 tcctacaaat aaagttcttt ttaatttcat ttttattacc ctcctagatg ttttatacta
     8401 caaaaaattt taataatttt ctttcttaat ttagtatttc taaattttta ttttttattt
     8461 actaaaatta aacaagttat aatttatttt tttggaaatc ataatgaata aataattaaa
     8521 taaaaaaatt ataattttat tattgatatt gattatcatt tatagtataa tctaattact
     8581 tgatattaat tttcatttga aggaggttat cttttatgtc ttgctgtaat tcacaatttt
     8641 gttgtaaaaa caaaattgaa tcatcatata taaaatggct tttagaagtt ttaggtccag
     8701 ttttcttagg ctctaaacct gctgaaataa taaatatttc atttactgat ataaatagag
     8761 aggaaaaaat taatgatatt tacaaatacc tatataaatg taagaaaata gattttatag
     8821 ttattgataa gaaagaaaaa ggattaaaaa tattatttgt aaacaaaaag gcattatcag
     8881 aaaaattaaa atgtaaaaaa acagtaaact ttttaaagtt tttaggatac aaacaaaata
     8941 cgaatgttaa tgcttattta gaacatttag tagataaact taagggtgat gtttttcctg
     9001 atgaaatagg tattttttta ggatatcctc ttaaagatgt gattggattt atgggatata
     9061 gtaattatga agtttctatg attaaatatt ggaaagttta tggtgacaca aaacaatcag
     9121 aggacacata ttcaaagttt ttacttcacc gtaagaaaat gagaaaatta ctagactaca
     9181 taagtgtaga taaaatagta agttgttttt aatagtgttt tgtatgataa taaaaataca
     9241 ccctctttta atatacagtt tacataataa gtaagggtgt attttctact ttaatatttt
     9301 aaaatattct aattctgatt gtacaacata tctttaatat ttattttctt ttgttgcatt
     9361 ccattagaca tcaaaaattc ctgtgtatct tctaattctt ttatatcctt atccgttatt
     9421 ttagaactaa agtcatacca agaatacatt tcttttgttt catctaaaga taaaccaact
     9481 tctttagata caacatccat cactttgtcc ttattttcat tcatatattt taaagtttct
     9541 tcatgaacct tcataaatct ttttactaat tcaggatatt tttcagcaaa gtcatcactt
     9601 acagcagtaa ctattattcc acttactaga ccttctccat ttacaatcat tttggcacct
     9661 gaatttatcg ttttcaatgc cactggacct gcaagcattg ctacatcagc acttttactt
     9721 tctaatgcag aagcagcact aggaatatcc atatttacat attcaacatc atctatagta
     9781 tagccttctt tagcaagtgc agcaatcaac agttgatgaa gtatagtccc ttttggccct
     9841 acaattttct taccttttaa gtcttttata gattttatat tttcattatt agttataagc
     9901 atatatcctt ttggtgaacg actataaata ttcgtaattt taagaccaac accatttgac
     9961 gcagctatta ttgcagatgt tcctcccaat gcatgtaaaa agtctagctc acctgctgct
    10021 agtgcttgag tttgctctgg tcctgttgtc aattcatgga agttgacttt tatattgtcc
    10081 tttttaaatt cctttccaaa taaatcatcc tttttttcta atatactagg aacattaagt
    10141 ggtgatttta cataagttaa atttatttcc ttaggtaaac tagcctcttt ttttgcaggt
    10201 tcatctttct tatcatttgt tgttgagcat cctacagtcc caaccaacac cattattaaa
    10261 gatgtcatta ctgctaaaat ctttttgttt cttttaatca attaaaactc ccccaattcc
    10321 cttaatattt cttttttcaa gtttatataa tagtttttag ttaaatctct attatattca
    10381 tcttcgacac taaactgttt aatcctttta tttttactaa aaacaattat tttctttgct
    10441 atttccatag cttcttcaat attgtgagtt acaaatacta cacctttttt agtattttta
    10501 tggatactta caacttcttt ttgcattttt cttcttgtga agtaatctag tgctgcaaat
    10561 ggttcgtcca tcagcaaaat atctggattg aaagaaagtg ctcttgctat acttactctg
    10621 tgagccattc ctcctgataa ttcatttgga tatgagtttt taaatttttc aagcttcatc
    10681 atttttaaat acttgtctaa atcaacttta tttctattat ccttttcagt atgtaaaagt
    10741 atattttcac ttacattcag ccatggcata agtctacttt cttgaaatac cattcctact
    10801 tttggagtac attttttatc attattaaaa aaatatacgt ttccagtagt agcattttca
    10861 agccctgcta aaattctaag tagtgtcgtc ttcccacatc cactttcgcc taatataaca
    10921 gttatctctt cactagatat atttaaagac acattatcaa gaactaaatg ctctttatta
    10981 tcaaccaaat atttcttaca tatattctct agcaaaaatc cacttctaac catttaaatc
    11041 aacctgcttc cctttgctaa attttgttat tatctttgaa aacagataat ctgaaagtat
    11101 gcctaaaaat ccaataacaa aaattcctac caagacaaca tcagttctag acatatcttt
    11161 tccatcagaa atcaaatatc ctaatcctga agaagctgca ataagctctg ctccaattat
    11221 agccctgaaa ctatacccaa gccctaagct aagtccaaca acaatatcca gcatagaatt
    11281 aggaattatt attttataaa aaattctctt cctagataaa ttaaaagatt tacctacttc
    11341 tattaattta ttgtcacaat tttttatgcc tttcaatgta tttaaaaata ttggaaaaaa
    11401 ggatgccaaa ataataatta taattttaga ttcttcacct ataccaaacc ataaaataag
    11461 cattggcacc aaagctagtg gaggtgtact tcgcataaac tcaaagagtg gcttgaaata
    11521 ttcatacatt tttttattta ctccaaatac aactccaagt ggtatagcca tcaatgaact
    11581 tattaaaaaa cctatcaata ttcttcttac actggcataa acatttaaga aaatagaacc
    11641 atcatatatc attttaatga aagtatctaa aactatttca ggaggaggca gaatatagct
    11701 actccatata cccagaacgc atactatctt ccataaaatt aaaattataa aaaaaatata
    11761 atatccttta aactttttat gaattttctc aaacatataa aactcctttt aacatatcta
    11821 cccctaatac cctttttatt ttttaatatc ctaaataata actaatctat atcataaaat
    11881 ttaatctatg ccaatcctta atccacctga gctcattcta tcagaaggag tagagtctgt
    11941 ttttaaacca gacaatcttt cagatggctg tataatgact atttgtccag gttcaacaaa
    12001 ttccatatga taagattctc ctgaagtctg accgataaaa gtcttccatg acaattttga
    12061 cactttagga tatggttctc ttccagtcca agctactaat gcatctgggt caacacaaca
    12121 gggtccttca agtattaaag ggttgccatc tgttattata gcaacatctt gattttgacc
    12181 tgttttattt gctaaatgtg tagtaaaaag acctttttga gatattacac ctgaaccaat
    12241 caattttaca ctgtaattta attgttcaga aaacgccaat aagttttcac tctcaacata
    12301 aatggagtca cctgggtcaa ttgatattac atctacatga taagcatttt gagctaggta
    12361 aatttcacct tcaccttcaa caaccattat tggcatttgt tctcctgtca atgacctctg
    12421 aacatgacct aataatgctc ttcccaagcc accaccatta cttggaccta acagcaattt
    12481 ttcaaaatta aaatttcctt taaaagctac catagcacct ttttttgcaa aaaatttacc
    12541 acttcctctt gctatacaca taagctcatt tgtcacctga acctcccatg aataaagtca
    12601 cagagttcct gcttcacaga attttgcata ctaaaatatg tcttactaat tctccacagt
    12661 ctatccccat aatttctacg gttcttacat aagctattta ttttgaattg ttattagtct
    12721 taatccttct tttaatatat ttagacttac tttaatgatt ttatcatatt taatatacta
    12781 tataatgctg ttgggaacaa tttattcatc atctatcata atattaacaa atgtaattat
    12841 ttaaaacaat tattcgaagg ttttatattt a
//
LOCUS       DAFNNB010000110        12868 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0111, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000110 DAFNNB010000000
VERSION     DAFNNB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0111"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            223..936
                     /gene="ispD"
                     /locus_tag="L3M60_002571"
     CDS             223..936
                     /gene="ispD"
                     /locus_tag="L3M60_002571"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBN6227222.1"
                     /translation="MYSVIIVAAGSGRRMHLDINKQFIKLREKEIIAHTIQVFYENIN
                     IDEIVVCIKKEEEDFFKENIINKYNFKNIKIAYGGKERQDSIYNGLKELDKNCDIVLI
                     HDGARPFVDHRIINESIKVAKEKKAVVVGVPVSDTIKIVSDGTVQETPERSLLWAAQT
                     PQTFEYNLIIDAYEQAYKNNYYGTDDSMLVENIGQSVTMVMGSYENIKITSPEDLNIA
                     EQILNMEKRDEVNSRRRII"
     gene            933..1418
                     /locus_tag="L3M60_002572"
     CDS             933..1418
                     /locus_tag="L3M60_002572"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBN6227223.1"
                     /translation="MRIGLGYDVHKLTEDRKLIIGGVEIPHDKGLLGHSDADVLIHAI
                     MDSILGALALGDIGKHFPDTDEEYKGADSMKLLEHVYNLITSKGYKIGNIDSTIIAQS
                     PKMAPYIENMRSNISKVLNTDIDNINIKATTEEGLGFTGAKQGIASQSICLLLLTSQN
                     N"
     misc_binding    1513..1720
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1784..3499
                     /locus_tag="L3M60_002573"
     CDS             1784..3499
                     /locus_tag="L3M60_002573"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBN6227224.1"
                     /translation="MKMSKMFMPTLKEVPADAEITSHQLMVRSGMIKKMTSGVYNQLP
                     MGLRVFKKIEQIIREELNKKDCQEILCAALLPSELWKESGRWTAMGEEMFRLKDRTER
                     EYCLGPTHEEAFTDIIRQEITSYKQLPLNLYQIQVKYRDERRPRFGVMRTKTFTMKDA
                     YSFDVDDKGLDKSYQDMFDAYVSIFDRCGLENSPVQADSGAIGGSTSTEFMVKSEVGE
                     DEVVFCSGCDYAANVERAESCNVASEKEEMKELEEVYTPGAATIKELEEFLKTSPDKF
                     AKTLVYEADGKTVVVVVRGDREVNEIKVSNAIGSVIEFALATDDVVRKVTNAEVGFAG
                     PIGINADYVFIDKEIVEQRNIVVGANKTEYHIKNANYGRDFEGVVGDFRNVQEGDKCI
                     VCGKPLEIARGVEVGHIFKLGTKYSESMNANFIDRDGKSKPIVMGCYGIGVERTAAAI
                     IEQHNDEKGIIWPLSVAPYHVVIVPANMKNEEQISIAEKIYNDLQAMGVEVLLDDRDE
                     RIGVKFNDSELIGIPMRITVGKNINEGKVEFKFRHKEDKEIIDIEEINEKVKAEFIRN
                     NVRLG"
     gene            3621..5066
                     /locus_tag="L3M60_002574"
     CDS             3621..5066
                     /locus_tag="L3M60_002574"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBN6227225.1"
                     /translation="MAKNEKQFVEEITKMEDDFPQWYTDVITKTDLVDYAPVKGFMVV
                     KPYGYALWEKMQEFMDKKFKETGHKNCYFPLLIPESLLNKEAEHVEGFAPEVAWVTHG
                     GNKKLEERLCVRPTSETIICTMYAKWLKSYRELPYLYNQWCSVVRWEKSTRPFLRTSE
                     FLWQEGHTLHETAEEAQEETIQQLEVYKALCEELLAMPVVAGQKSESEKFAGGERTYT
                     IEAMMHDGKALQSGTSHFLGQHFTKAFDITFADREGNLANPYHTSWGASTRLIGGLIM
                     THSDNRGLVLPPRVAPIQVVIVPIAAKKGNVMETVDKIYADLKAKGVAVEVDDRDNYT
                     TGWKFNEWEMKGVPVRIEIGPKDIENNQAMVFRRDTLEKDSMPLEGLADAVCDLFDVI
                     HNDMFEKARKHREDNTSIVENMDEFRKALEEKPGFIKTMWCGDAECEAKIKEETGATI
                     RCLPFEQENLGHKCVYCGKEADSMVVMAKAY"
     gene            5510..6991
                     /locus_tag="L3M60_002575"
     CDS             5510..6991
                     /locus_tag="L3M60_002575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBN6227226.1"
                     /translation="MNVRVRFAPSPTGFVHIGSLRTALYNYLFAKKMGGEYILRVEDT
                     DQSRLVEGAIENMLNAMKWAGVNHDEGVVLDDSGKVVQKGEYGPYIQSQRLDIYQEYI
                     KQLLDSGKAYYCFCTKERLDEVRDAQRAAGETAKYDGHCKNLSKEEVEANIKAGIPYV
                     IRLRLPENHTIKFTDLVRGDMEFNTNDLDDQVLMKTDGFPTYHFAVVVDDYLMKITHV
                     IRGEEWVSSTPKHVYLYEAFGWEAPVFVHLPNILNKEKKKLSKRQGDVAVEDFKKKGY
                     LPEGLVNYVALVGWSPEDNQELFTMEELEKAFSIERVSKSGGVFDTEKLNWVNQHYIK
                     DGDDAYLTDLAIPFLIEDGFITEEEATNKYDFLKSMISVLKEKLQYVKEVTEHASIFF
                     GDKIEVETEEGREFLRLEHIPTLIDALKEKIEKAEVLNAEFVQAMLKEIQKEYKIKGK
                     NLFMGSRIILTGQMHGPDLPKVMEVLGKETCLNRIAYVKNNIL"
     gene            7212..7367
                     /locus_tag="L3M60_002576"
     CDS             7212..7367
                     /locus_tag="L3M60_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227227.1"
                     /translation="MYSNIDDVKKELKELCLEYVTILEKLKDEKMITEETFEKCSSQK
                     KIFLEEQ"
     gene            7512..8909
                     /locus_tag="L3M60_002577"
     CDS             7512..8909
                     /locus_tag="L3M60_002577"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBN6227228.1"
                     /translation="MKVYNTLTRTKEEFVPLEEGKVKMYVCGPTVYNYIHIGNARPFI
                     IFDTLRRYLEYRGYDVTYVQNFTDVDDKIINRSHEEGISPEEVAAKYIKEYFVDCDGL
                     GIKRATVHPQVTDNIQQIIEFIKELEDKGYAYAVNGDVYFDTNKFEGYGKLSGQKQED
                     LEAGARIEVNDQKRHPMDFVLWKAKKEGEPGWDSPWGEGRPGWHIECSVMSKRYLGET
                     IDIHAGGQDLTFPHHENEIAQSEARSGKTFSKYWMHNGYININDEKMSKSKGNFFTVR
                     DISKLYDLEIVRFFMLSAHYRNPVNFSDEMLNQAKAGLERLYNTKEKLEFTLSNLVES
                     PLTEKEVELVKELDGFRQKFIDAMDDDVNTADAVSVIFELAKLINSNVDENSSLEFAK
                     KCLDEFNELTGVLNIVNKKKDTVLDKDIEELIQKRTDAKKNKEFQLADDIRQQLLDMG
                     IVLEDTRQGVKWKRI"
     gene            8894..9307
                     /locus_tag="L3M60_002578"
     CDS             8894..9307
                     /locus_tag="L3M60_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HBN6227229.1"
                     /translation="MEKDIKMEKTELVTMSPLVLAYLGDTVYETYIREHLIRQNTQKK
                     VNDLHKLAIKYVKAKAQATIIHEIEIELTEEESKIYKRGRNQKSNTSPKNADIIDYKH
                     ATGFEALVGYLYLNNEIERLQYIINKGIKIIERDM"
     gene            9413..10171
                     /locus_tag="L3M60_002579"
     CDS             9413..10171
                     /locus_tag="L3M60_002579"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HBN6227230.1"
                     /translation="MKVKLISHTPEPEKVIAMAAKLCYSPVGTDEIEKDLTDESIEKF
                     LNMLLSIGHGSILEHASFTFSIEGISRACSHQIVRHRIASFSQQSQRYVKLEQFEYII
                     PPEIEKIEKAKELFIDSMKKDQENYDKLVEILFENHYNDLIKNGKNEKTAKRQAEKKA
                     IEDARYVFPNACETKMVFTINARSLFNFFEHRCCERAQWEIRNLAVEMLREVKKVAPI
                     LFKKTGPSCVNGSCPEGTMTCGDIVQVREKFKAL"
     gene            10184..10921
                     /gene="rlmB"
                     /locus_tag="L3M60_002580"
     CDS             10184..10921
                     /gene="rlmB"
                     /locus_tag="L3M60_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBN6227231.1"
                     /translation="MANIEGRNPVIEAIKSDREIDKILIANSAKEGSIKKIIGMAKDK
                     NIIIQYVDKHKLDEVSTSHSHQGVIAYASEHKYYELDELIDLVKNKDEDPFFIILDEI
                     TDPHNLGSIIRTADAVGAHGVIIPKRRSVHITPVVAKASAGAVEYMPVCKVTNIVNTI
                     KRLKEEGLWIAAADMDGETFYKQNLTGPLGVVIGSEGFGISRLVKQNCDFIVKMPMIG
                     NVTSLNASVAGGILLYEIFRQRLGKSK"
     gene            10924..11451
                     /locus_tag="L3M60_002581"
     CDS             10924..11451
                     /locus_tag="L3M60_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HBN6227232.1"
                     /translation="MKKNINHYLIIDGYNIINAWDNLKELAKEDLEDSREKLIDDIIE
                     FSEFMGYKTIIVFDAYNVKNSREKVEKRKHITIVYTREHQTADSYIEKFITSLSKYDD
                     VKVATNDYAEQQMILGKGATRMSARELKLELDRSKNKMKEKNIGLRKKIQRNWLEERL
                     DKETLSKLENIRRKR"
     gene            11516..12163
                     /gene="sigH"
                     /locus_tag="L3M60_002582"
     CDS             11516..12163
                     /gene="sigH"
                     /locus_tag="L3M60_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HBN6227233.1"
                     /translation="MLVAKEKSYELVDNCQQDEYNIVLRASEGDKIALEYIITKYRNF
                     VKAKAKSYFLIGADKEDIIQEGMIGLYKAVRDFDGSKTNSFKCFAEICITRQIITAIK
                     TATRQKHIPLNSYVSLNKPIYDEESDRTLLDIIATSIVTDPEELIISKEELKNIESKM
                     NELLSDLELEVLELYLNGKSYQFIADKLKRDVKSIDNALQRVKRKLEKHLENRND"
     gene            12237..>12868
                     /locus_tag="L3M60_002583"
     CDS             12237..>12868
                     /locus_tag="L3M60_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBN6227234.1"
                     /translation="MAKAKYERTKPHVNIGTIGHVDHGKTTLTAAITKTLYDRYQLGE
                     AVDFANIDKAPEERERGITISTAHVEYETPNRHYAHVDCPGHADYVKNMITGAAQMDG
                     AILVCSATDGPMPQTREHILLSRQVGVPYIVVFLNKCDMVDDEELLELVEMEVRDLLT
                     EYDFPGDDTPIVRGSALMALEDPKSEWGDKIVELFEQIDEYIPAPERDTD"
BASE COUNT         5365 a         1317 c         2529 g         3657 t
ORIGIN      
        1 atatttttac atatcaataa ccattaaaaa attgtaaaat taaaaacaat actattgaaa
       61 aatcaatata tattgtaata aatatacatg tttttcatta gtaaatagac attagctatt
      121 tatacaaccg aaaatttaat atataattaa tcagatggtt aaaataaaaa atattaaaag
      181 ttttaataaa tgatggttaa aaataaaata cgaggtgata ccatgtatag tgttataatt
      241 gttgcggcag gaagtggaag gagaatgcac ttagacataa acaaacagtt cataaaatta
      301 agagaaaaag aaattattgc tcatacaatt caagtttttt atgaaaatat aaatatagat
      361 gaaatagtgg tatgtataaa gaaagaagaa gaagattttt ttaaggaaaa cataataaat
      421 aaatataatt ttaaaaacat aaaaatagca tatggtggaa aagaaagaca ggattcaata
      481 tataatggtt taaaagaatt agataaaaat tgtgatatag tacttataca tgatggagca
      541 aggccatttg tagaccatag aataatcaac gaatcaataa aggtagctaa agaaaaaaag
      601 gctgtggttg taggtgttcc agtaagtgat actataaaga tagtatcaga tggaacagta
      661 caagaaactc cagaaagaag tttgctgtgg gcagcacaaa cccctcaaac atttgaatat
      721 aatctcataa tagatgctta tgaacaagca tataaaaata attattatgg tactgatgat
      781 tcaatgttag tagaaaatat aggtcaaagt gtcactatgg taatgggttc atatgaaaat
      841 ataaaaataa ctagtccaga agatttaaat atagcagaac aaatattaaa tatggaaaaa
      901 agagatgaag ttaatagtag aaggagaatt atatgagaat aggattagga tatgatgtac
      961 acaaattaac agaggataga aagcttataa tcggcggtgt agagataccg cacgataaag
     1021 gtttgttggg gcattctgat gctgatgtat tgatacatgc aataatggat tctattttag
     1081 gtgctctagc attaggagat atagggaaac atttcccaga tacagatgaa gaatataagg
     1141 gagcagatag tatgaaatta ttagaacatg tgtataatct aataacaagt aaaggatata
     1201 aaattggaaa tatagatagt actataattg ctcaaagtcc taagatggct ccatacattg
     1261 agaatatgag aagtaatata tcaaaagttt taaacacaga cattgataat ataaacataa
     1321 aggcaacaac agaagaaggt cttggtttta caggagcaaa acagggaata gcttcacaaa
     1381 gtatttgttt attattgttg acaagtcaga ataattaata tattattagt aataagttta
     1441 atggatatca agtaataggc ctatatagaa tatctataat gtgtgaattt aagtgaaatt
     1501 aatataaata taaaattagc tatgataaag aaatagtaaa tgatgaaatc ttcacagaga
     1561 ggaaacggtg gtgagagttt tcaagaggaa gtctttgaac ctgtctttta gcttaaaggt
     1621 ataaaaatct ttacggtagg aaccgttata ttcccaaaag agagctattc atatagctaa
     1681 ctagagtggt accgcggaaa ttaaaccttt cgtctctata cttgtataga gatgagaggt
     1741 tttttatatt ttcagaaagt taatattcag gaggaatcta ttaatgaaaa tgtcaaaaat
     1801 gtttatgccg acattaaaag aagtcccagc agatgctgag attacaagcc atcaattaat
     1861 ggttaggtca ggcatgataa aaaaaatgac ttcaggtgtt tataaccaat taccaatggg
     1921 gttaagagtg ttcaaaaaaa ttgaacaaat aattagagaa gaattaaata aaaaagattg
     1981 tcaagaaata ctttgtgcag ccttacttcc ttctgaatta tggaaagagt ctggaaggtg
     2041 gactgctatg ggtgaagaaa tgtttagatt aaaagacaga acagaaagag agtattgtct
     2101 tgggccaaca catgaagaag cattcactga cataataaga caagaaatta cttcatataa
     2161 acaattgcca ctgaacttgt accaaataca agtaaaatat agagatgaga gaagaccaag
     2221 atttggtgtt atgagaacta agacttttac gatgaaggat gcttatagtt ttgatgtaga
     2281 tgataaaggt ctagataagt catatcaaga tatgtttgat gcatatgtaa gtatatttga
     2341 tagatgtgga ttggagaata gccctgtaca ggcagattca ggagctatag gaggttctac
     2401 atcaactgaa tttatggtta agtcagaagt tggtgaagat gaagttgtat tttgtagtgg
     2461 atgtgactat gcagccaatg tagaaagagc agaatcttgt aatgtagcat cagaaaaaga
     2521 agaaatgaaa gaattagaag aggtttatac accaggagca gctacaataa aagagctaga
     2581 agaattttta aaaacttctc cagataagtt tgctaaaact ttagtatatg aggcagatgg
     2641 aaaaacagtt gtagtcgttg taagaggtga tagggaagtt aatgaaatca aagtatctaa
     2701 tgctattggc tctgttatag aatttgcact tgctacagat gatgtagtga gaaaggtgac
     2761 taatgcagaa gtaggatttg caggaccaat aggtataaat gctgattatg tatttataga
     2821 taaagaaatt gttgaacaaa gaaacatagt tgttggggct aataaaactg agtaccatat
     2881 aaaaaatgct aactatggaa gagattttga aggagtagtt ggagacttta gaaatgttca
     2941 agaaggcgac aaatgtatag tatgtggtaa gccacttgaa atagctaggg gtgttgaagt
     3001 aggacatata tttaaactag gaactaagta ctcagaatct atgaatgcaa acttcataga
     3061 cagagatgga aaatcaaagc ctatagtaat ggggtgttat ggaataggtg ttgaaagaac
     3121 agcagcagct ataattgaac aacataatga tgagaaaggt ataatatggc ctttatcagt
     3181 agctccatac catgttgtga tagttccagc aaatatgaag aatgaagaac aaatatcaat
     3241 agctgaaaaa atatataatg atttacaggc tatgggtgta gaagttttat tagatgacag
     3301 agatgaaaga ataggtgtca aattcaatga ttcagaatta ataggaatac ctatgagaat
     3361 tacagtaggt aaaaatatta atgaaggtaa agtagaattt aaatttagac ataaagaaga
     3421 taaagagata attgacatag aagaaataaa tgaaaaagta aaggcagagt ttatcagaaa
     3481 taatgttagg ttaggataat aacttaaata agatattgta tagataatca gagttgtact
     3541 ttatagacac cattttatct atatataagg atataaaatg gtgttttttt ataaaacaaa
     3601 taaatgagga ggtaattgaa atggcaaaaa atgaaaaaca atttgttgaa gaaataacaa
     3661 agatggaaga tgattttcca caatggtaca cagatgtaat aactaagact gacttagttg
     3721 attatgcacc agtaaaaggt tttatggttg taaagccata tggatatgca ttatgggaaa
     3781 agatgcagga atttatggat aaaaaattta aagaaactgg acacaaaaac tgttattttc
     3841 cattattaat accagagagt ttattaaaca aagaagcaga acacgttgaa ggttttgctc
     3901 cagaagttgc atgggtaaca catggaggaa ataaaaaatt agaagaaaga ttatgtgtaa
     3961 gacctacttc agagactata atatgtacaa tgtatgcgaa gtggttaaaa tcatatagag
     4021 aattaccata cctttataat caatggtgct cagttgttag atgggaaaaa tcaactagac
     4081 ctttcttaag aacatctgag tttttatggc aagaaggtca tacattacat gaaacagcag
     4141 aagaagcaca agaagaaaca atacaacaat tagaagtata taaagcacta tgtgaagaat
     4201 tacttgcaat gccagttgta gctggtcaaa aaagtgaaag tgagaaattt gcaggtggtg
     4261 aaagaacata tactatagaa gctatgatgc atgatggtaa agcattacaa tcaggtacaa
     4321 gtcacttctt aggtcaacac tttactaagg cttttgatat tacttttgca gatagagaag
     4381 gaaacttagc taatccatat catacatctt ggggagcatc aacaagactt ataggtggac
     4441 ttataatgac tcatagtgat aacagaggtt tagtattacc accaagagta gctccaattc
     4501 aagttgttat agttcctata gctgccaaaa aaggaaatgt aatggaaact gttgataaaa
     4561 tatatgctga tttaaaagct aagggagtag ctgtagaagt tgatgataga gataactata
     4621 caactggatg gaaatttaat gagtgggaaa tgaagggtgt tccagtaaga attgaaattg
     4681 gaccaaaaga tatagaaaac aatcaagcta tggtatttag aagagatact ttagaaaagg
     4741 attctatgcc tttagaagga ttagctgatg ctgtatgtga tttatttgat gttatacaca
     4801 atgatatgtt tgaaaaagca agaaaacata gagaagacaa tacatctata gttgaaaata
     4861 tggatgagtt tagaaaagct ttagaagaaa agccaggatt cataaagact atgtggtgtg
     4921 gagatgcaga atgtgaagct aagattaagg aagaaacagg agcaactata agatgcttac
     4981 cttttgaaca agaaaactta ggtcacaagt gtgtgtactg tggaaaagaa gcagatagta
     5041 tggtagtaat ggcaaaagct tattaaaata atacatttaa ataaaaactg gggtagaaag
     5101 tatttaatac tttatcccag tttttttatt ttttatgaga aataaaatcg tgtttgtaga
     5161 tgtatcataa gataagtcat aaatgcttat attaaaaaga atataataga gaactatttt
     5221 taataattac atattaatga aagaaaaata tgtttgatat gtaaattttc aataaagtaa
     5281 acaataaata taattgaata ttaataaaga gtacttttaa tttttagaaa ataaatgact
     5341 taaaagaaag atttatgaaa ttaaaactag gattttctga tataataaac atgtgtattt
     5401 ttaaataaaa aggatattat tattagattt gattaaaaat aaacttgcaa aacaatgtac
     5461 tagtttaata ttatagttat attgaataaa atatcaggag gaagtaaaaa tgaatgtaag
     5521 agtaaggttt gcaccaagtc caacgggatt tgtacatata ggtagtttaa gaactgcttt
     5581 atataactat ttatttgcaa aaaaaatggg cggagaatat atattaagag tggaagatac
     5641 agaccaaagt agattagtag aaggtgcaat agagaatatg cttaatgcta tgaaatgggc
     5701 tggtgtcaac catgatgaag gtgttgtatt agatgatagt ggaaaagtgg tacaaaaagg
     5761 agaatatggt ccatatattc aatctcaaag attagatata taccaagaat atataaagca
     5821 acttttggat agtggaaagg cttattactg tttctgtaca aaagaaagac tagatgaagt
     5881 aagagatgct caaagagcag caggagaaac agctaaatat gatggtcatt gtaaaaatct
     5941 ttccaaagaa gaagtagaag caaatataaa agcaggaata ccatatgtta taagattaag
     6001 attgccagaa aatcatacaa ttaaatttac agatttagtt agaggagata tggaatttaa
     6061 cactaatgac ttagatgacc aagtattaat gaaaacagat ggatttccaa cttatcattt
     6121 tgcagtagtt gttgatgact atttgatgaa aattacacat gttataagag gagaagaatg
     6181 ggtatcatct actccaaaac atgtttattt atatgaagct tttggatggg aagcacctgt
     6241 atttgtgcat ttaccaaata tacttaataa agaaaagaaa aaattaagta aaagacaagg
     6301 tgatgtagca gttgaagact ttaagaaaaa aggatattta ccagaaggat tagtaaacta
     6361 tgtagcatta gttggttggt ctccagaaga taatcaagaa ttgtttacaa tggaagaatt
     6421 agaaaaagcc ttctctatag aaagagtatc taaaagtggt ggagtatttg atactgaaaa
     6481 gctaaattgg gttaatcagc attatataaa agatggtgat gatgcttacc taacagactt
     6541 agcaatacca tttttaatag aagatggatt tataacagaa gaagaggcta ctaataaata
     6601 tgatttctta aagagtatga tatcagtact taaagagaaa ttacaatatg taaaagaggt
     6661 tactgagcat gcaagtatat tttttggaga taaaatagag gtagaaactg aagagggaag
     6721 agagttctta agattagaac atatacctac tttaatagat gctttgaaag aaaaaataga
     6781 aaaagcagaa gtattaaatg ctgagttcgt tcaagctatg cttaaagaaa tacaaaaaga
     6841 gtacaaaata aaaggaaaaa atttatttat gggctcaaga ataattttaa cagggcaaat
     6901 gcatggtcca gatttaccaa aagtaatgga agttttagga aaagaaactt gcttaaatag
     6961 aatagcttat gtaaaaaata atatattata gtcataaaag tccgctaatt tagaagcgga
     7021 cttttttcgt gttagtattt ggtgaaaata tctgcttaag aaaatgaagg tatttaaggt
     7081 aataaattta atatataaaa agcaaagcaa aaatataaat aaaatatgaa taggaaacta
     7141 ataaaatatt aagcatagaa gaagggaatc tattatttta gtttgattta attattaagg
     7201 gggtaattat tatgtatagt aatattgatg atgttaaaaa ggaattaaag gagctatgct
     7261 tagaatatgt tacaatttta gaaaaattaa aagatgaaaa aatgattaca gaagaaactt
     7321 ttgaaaagtg ctcttctcaa aaaaagatat ttttagaaga acaatagagg atagtaatag
     7381 tattattatt atgtagtttg aaagatagta cgaaaaatga tagtaatact attatttatt
     7441 gcaattttca actataatat aatatataat gttaagaaga taatttataa aaatacagtg
     7501 aggtgatgag tgtgaaagtt tataatacat tgactagaac aaaagaagaa tttgttccat
     7561 tagaagaagg aaaggtaaaa atgtatgtgt gtggtccaac tgtttataac tatatacaca
     7621 taggtaatgc aagaccattt ataatatttg atactctaag aaggtattta gagtatagag
     7681 gatacgatgt aacttatgta caaaacttta cagatgttga tgataagatt ataaatagaa
     7741 gtcatgagga aggaatatct cctgaagaag ttgctgctaa gtatataaaa gaatattttg
     7801 tagactgtga tgggctagga attaaaagag ctactgttca tcctcaggtt acagataata
     7861 tacaacaaat tatagagttt ataaaagaat tggaagataa gggatatgca tatgcagtaa
     7921 atggtgatgt atattttgat acaaataaat ttgaaggata tggaaagctt tcaggtcaaa
     7981 aacaagaaga tttagaagca ggggctagaa ttgaggttaa tgaccaaaaa agacatccta
     8041 tggattttgt tctttggaaa gcaaaaaaag aaggtgagcc aggatgggat agtccttggg
     8101 gagaaggtag accaggatgg catatagaat gttctgttat gtctaagaga tatttaggtg
     8161 agacaattga tatacatgca ggtggtcagg atttaacatt cccacatcat gaaaatgaaa
     8221 tagctcaaag tgaagctaga agtggaaaaa cattctcaaa atactggatg cataatggat
     8281 atataaatat aaatgatgaa aagatgagta aatcaaaagg taatttcttt acagtaagag
     8341 atatatcaaa actatatgat ttagagatag taagattttt catgttatca gcacattata
     8401 gaaatccagt aaactttagt gatgagatgt taaatcaagc taaagcaggt cttgaaaggt
     8461 tatataatac taaagaaaaa ttagagttta cgttaagtaa tttagtagaa tctcctttaa
     8521 cagaaaaaga agtagaactt gttaaagaat tagatggatt tagacaaaag ttcatagatg
     8581 ctatggatga tgatgttaat acagcagatg cagtaagtgt aatatttgaa ttagctaagt
     8641 taataaattc taatgtagat gaaaattctt ctctggagtt tgctaaaaaa tgcttagatg
     8701 aatttaatga acttacagga gtattaaata ttgtaaataa gaaaaaggat actgtattag
     8761 ataaagacat tgaagaattg atacaaaaaa gaactgatgc aaagaaaaac aaagagtttc
     8821 aattagctga tgatattaga caacaattac tagatatggg aattgtatta gaagacacaa
     8881 gacaaggtgt taagtggaaa aggatataaa aatggaaaaa accgaattag ttactatgtc
     8941 accactagtt ttagcatact taggtgatac agtttatgaa acatatataa gagaacattt
     9001 aattagacag aatacacaaa aaaaagttaa tgatttgcat aaattagcta taaaatatgt
     9061 aaaagctaaa gctcaggcaa caataattca tgaaattgaa attgaattaa ctgaggaaga
     9121 gagtaaaatt tataaaaggg gaagaaatca aaagtcaaat acttctccta agaatgcaga
     9181 tattatagac tataaacatg caactgggtt tgaagcgcta gtgggatatt tgtatttaaa
     9241 taatgaaata gagagacttc aatatattat caataaagga atcaaaatta ttgaaagaga
     9301 tatgtaaata ttaaagagtg ttgtcttaat aataagtaat aaggcaacac tttttttata
     9361 actaatgtat aataaatata tagaaattag taatgagaaa ggataaaaaa ttatgaaagt
     9421 aaaattaata tcacatacac ctgaaccaga aaaagtaata gctatggcag ctaagctatg
     9481 ttattcacca gttgggacag acgaaataga aaaagactta acagatgaaa gtatagagaa
     9541 atttttaaat atgttattaa gtataggtca tgggtcaata ttagaacatg catcatttac
     9601 attttcaata gaaggtattt caagagcttg ttcacatcaa attgttagac atcgtatagc
     9661 tagtttttca caacaaagtc aaagatatgt taaattagaa caatttgagt acataattcc
     9721 gccagaaata gaaaaaatag aaaaggcaaa agagttattt atagactcta tgaaaaaaga
     9781 ccaagagaat tatgataagt tagttgaaat attgtttgaa aatcattata atgacctgat
     9841 aaaaaacggt aaaaatgaga aaacagctaa aagacaagct gaaaagaaag caatagaaga
     9901 tgctagatac gtatttccaa atgcatgtga aacaaaaatg gtatttacta taaatgctag
     9961 aagtttgttt aatttctttg aacatagatg ctgtgaaaga gcacagtggg aaataagaaa
    10021 tttagctgtg gagatgttaa gagaggttaa aaaggttgct cctatattat ttaaaaagac
    10081 gggtccaagt tgtgtaaatg gaagttgtcc agaaggaact atgacctgtg gagatatagt
    10141 tcaagttaga gaaaaattta aggccttgta ggaggtgaat agtttggcaa atatagaagg
    10201 aagaaatcca gtaatagaag caataaaaag tgacagagaa atagataaaa tattaattgc
    10261 aaattcagct aaagaaggtt caattaagaa aataatagga atggctaaag ataagaatat
    10321 aattattcag tatgttgata aacataagtt agatgaagta agtacaagtc attcgcatca
    10381 aggtgtaata gcttatgcaa gtgagcataa gtattatgaa ttagatgagc taatagattt
    10441 ggtaaagaat aaagatgaag acccattttt cattatatta gatgagataa cagacccaca
    10501 taatttgggt tcaataataa ggaccgcaga tgcagtaggg gcgcatggag ttatcattcc
    10561 aaaaagaagg tctgtacaca taactcctgt agttgccaaa gcatctgctg gagcagtaga
    10621 atatatgcca gtttgtaagg taaccaatat agttaataca ataaaaaggc tcaaggaaga
    10681 agggttgtgg attgctgcag ctgatatgga tggagaaact ttttataaac aaaatcttac
    10741 tggtcctctt ggtgtagtaa ttggaagtga aggttttgga atctctagat tggttaaaca
    10801 aaactgtgac tttatagtaa aaatgccaat gataggtaat gttacatctt taaatgcatc
    10861 tgtagcaggt ggaatacttt tatatgagat attcagacaa agactgggta aaagtaaata
    10921 ggtatgaaaa aaaatataaa tcattactta ataatagatg gctacaatat tataaatgcg
    10981 tgggacaatt tgaaagaact ggctaaagag gacttagaag attctcgaga aaagcttatt
    11041 gatgatatta tagaattttc tgagtttatg ggttataaga ctataatagt ttttgatgct
    11101 tataatgtta aaaattctcg agaaaaagtt gagaaaagaa aacacataac aatagtttat
    11161 acaagagaac accaaacagc agatagttat atagaaaaat ttataacttc tttatcaaag
    11221 tatgatgatg taaaagtagc cactaacgac tatgcagaac agcaaatgat tttaggaaaa
    11281 ggtgctacta gaatgtctgc aagagaacta aagttagagt tagatcgttc caagaataaa
    11341 atgaaggaaa aaaatatcgg tttgaggaaa aaaattcagc gaaattggtt ggaggaaagg
    11401 ttagataaag aaacattgtc gaaacttgag aacattcgta gaaagcgttg aaattactag
    11461 gttctttaga ctataatata cctataaatt tgttttatat atgggggaga tttacatgtt
    11521 ggtagcaaaa gaaaaaagtt atgagttagt ggacaattgt cagcaggatg agtataacat
    11581 tgtattaaga gcaagtgaag gggataaaat agcactagag tacattatta ctaaatacag
    11641 aaattttgta aaggcaaaag cgaagtcata ttttttaatt ggcgcagata aagaagatat
    11701 aatacaagaa ggaatgatag gcctttataa ggcagttaga gattttgatg gaagtaagac
    11761 caattcattt aaatgttttg cagaaatatg tataacaaga caaataataa ctgctataaa
    11821 aactgcaaca agacaaaaac atataccttt aaattcatat gtttctttga ataaaccaat
    11881 atatgatgaa gagtcagaca gaacactttt agatattata gctacaagta tagtaacaga
    11941 tccagaagag cttataatta gtaaagaaga gttaaaaaat atagaatcaa aaatgaacga
    12001 attgttaagt gacttggaat tagaagtact agaattatat ttaaatggaa agtcatacca
    12061 gtttatagcg gacaagctta aaagagatgt aaagtcaata gataacgctt tacaaagggt
    12121 taagagaaaa ttagaaaaac atcttgaaaa cagaaatgat taatagtaaa atacttacca
    12181 taagcttatt gtgagcaaaa ttaatttata tcgaaagata agggaggaaa ttcaaaatgg
    12241 ctaaagctaa atacgaaaga acaaaacctc atgttaatat agggacaata ggacacgtag
    12301 accacggtaa aactacatta acagcagcaa taacaaaaac attatatgac agatatcaat
    12361 taggagaagc agtagatttc gcaaacatag ataaagctcc agaagaaaga gaaagaggaa
    12421 tcacaatatc aacagcacac gttgagtatg aaacaccaaa tagacactat gcacacgttg
    12481 actgcccagg gcatgctgac tacgttaaga acatgataac aggagcagca caaatggacg
    12541 gagcaatatt agtttgttca gcaacagatg gaccaatgcc acaaacaaga gagcatatac
    12601 tattatcaag acaagttgga gtaccatata tagtagtatt cttaaataaa tgtgacatgg
    12661 tagatgatga agagttatta gagttagtag agatggaagt aagagattta ttaacagagt
    12721 atgatttccc aggagatgac actccaatag taagaggatc agcgttaatg gcattagaag
    12781 atccaaagag tgagtgggga gataagatag tagaattatt cgagcaaata gatgaatata
    12841 taccagcacc agagagagat acagataa
//
LOCUS       DAFNNB010000111        12824 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0112, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000111 DAFNNB010000000
VERSION     DAFNNB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0112"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(320..889)
                     /locus_tag="L3M60_002584"
     CDS             complement(320..889)
                     /locus_tag="L3M60_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBN6227235.1"
                     /translation="MKLPIALSNKHVHLSQADIDVLFGAGHELTKFKPLSQPGQYACE
                     EKVDLVGPKGTLKGVRVLGPARAHTQVEISLADGFPLGVKAPIKESGDIAGTPGVKLV
                     GPAGEVEMKEGVIVASRHIHMSNEDAAKFGVTDKQIVKVKTSGPRALVFENVLVRASD
                     SFNLEMHVDMEEGNAAGVKNGDLVELIVD"
     gene            complement(961..1569)
                     /locus_tag="L3M60_002585"
     CDS             complement(961..1569)
                     /locus_tag="L3M60_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6227236.1"
                     /translation="MDSTDKKGLKSPEIMIINGSPRINKNCSFIANEVKAMLEEYGLS
                     CKIFNIYDMDIEYCTACGFCEKTGYCKFNDDMKPLYKMFDDSKGTIVISPVHFDSISA
                     KLKTVVDRTQAIYASKYILNKPSIDRNKKRIGMYIAVGGSKSYDSQFKGGQIVMDFFF
                     KSINTKLLHNIYLNDTDNVSYMSNQEFKDIIYKNLKRYIDSI"
     gene            complement(1577..3340)
                     /locus_tag="L3M60_002586"
     CDS             complement(1577..3340)
                     /locus_tag="L3M60_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HBN6227237.1"
                     /translation="MTNGSYKMKNLVEISAMVTESTDFFNIKDDIIEKMLEVVHPTKA
                     CVNLFYKNDSKYAYLVCSQTLEYVPQIFPINSLKGAKIDFDTYPEYIHEAVNEKKIIY
                     VENVFEDSRAEGERDLAKAEGYIGRIVFPFIINNVVVGFMTCFLKEDDYLTEQDIDFI
                     SSVASLLSLSIEITNKNNNVQILIDKLRGSISAINEATKKLYLNKSIDGFLENLSKQA
                     CNITNSKEALIIINSHHNKDAIVSCYNVEQKKKTNLYPMIDTFLKKESLGGYLNNLKP
                     CSKKGINLESYIYYKLQDKNETIGCIVCANSKNYTSDDLNILSILSKQVSVGMQLYEH
                     NEKEVKHKVLENELNILNKQQQLIMDKGKMDCNDEKELYFYHKPARVVGGDFYYAIKV
                     DDEHIVYIVADVMGHGMVSNYVVAMIKGAFKVLCNQYNTPREIMTNLNRMLYDEFDKM
                     GVFTTCLIGIIDTKENMITVSNAGHYSPVVVKKDGTIENNLNCKKGIPIGIMEDASYE
                     NNTISMEDYAMVCMYTDGILEIKNSSKEEYGINRLENFLKENFRLNQQLIVENLKLDL
                     KNFSSKDNYDDDILIVMLKDR"
     gene            complement(3383..3850)
                     /locus_tag="L3M60_002587"
     CDS             complement(3383..3850)
                     /locus_tag="L3M60_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227238.1"
                     /translation="MKMLNGIIKLFYSEIVKTKINNLDSSGYSLRDECKEIRNITLKI
                     DSIFFKLLKLAFGSILLMVAFNLNMVLGIGVFLVGVVYILYKRNMEEQVKEHINNVKN
                     NIDISKITFMDEKGRTGLNALITLLLIGLLTGFNWILVASFVVVFVFTIKNVC"
     gene            4033..4494
                     /locus_tag="L3M60_002588"
     CDS             4033..4494
                     /locus_tag="L3M60_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227239.1"
                     /translation="MQIEQLDLETRNKIYCYTKKILRKYQKGITSGKLTADKFADNIL
                     SQDFISSILDEKIVNETNFKISYRNYIETLINIQNENLSNFKKKSTKTAKCFNISQIT
                     QLKNLLNNTGYNLLIPYKYLTSRDIEGIVTLINTGSIELGNERIYNYISKA"
     gene            complement(4587..4757)
                     /locus_tag="L3M60_002589"
     CDS             complement(4587..4757)
                     /locus_tag="L3M60_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227240.1"
                     /translation="MSSVNFEDLKNKFINSDLDEKIRIYTTTEGLSVEQFKELLKYYP
                     IQHLSKLEKALG"
     gene            complement(4946..5167)
                     /locus_tag="L3M60_002590"
     CDS             complement(4946..5167)
                     /locus_tag="L3M60_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HBN6227241.1"
                     /translation="MASNNNNNRTVVPGAKEALNQMKLEIANELGMSNYQQVDKGNLT
                     ARENGYVGGYMTKKLVEMAEQQMAGKSNR"
     gene            complement(5251..6477)
                     /locus_tag="L3M60_002591"
     CDS             complement(5251..6477)
                     /locus_tag="L3M60_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN6227242.1"
                     /translation="MKKVVIIGGGPAGMIAASTACERGCDVTLVEKNHKLGKKLAITG
                     KGRCNITNACEIEELIENVPTNGKFLYSAFYTFTNEDVISMFNNLGVKTKTERGKRVF
                     PESDKAFDIVNALERQLKSKKVNILLNSKVEKIISKNNKIEKVILNNKKEIKCDSVVV
                     ATGGLSYPLTGSTGDGYKFAISQGHTIMDTKPSLIGIEVQESFTRDLEKLSLRNVEIR
                     VFNSKQKKVYSDFGELEFTKFGLDGPIIKSASCRMKDTRKENYTIILDLKPALDEEKL
                     DKRIQKDFQKYTNKKFEKALDDLLPKKLIPVIVNLSEINPDTVVHQISREQRKNLVHL
                     LKNLKFTVKRYRPIDEAIITSGGVKVNEINSSTMESKLVEGLFFAGEVIDIDAYTGGF
                     NLQIAFSTGYLAGFNC"
     gene            complement(6538..7470)
                     /locus_tag="L3M60_002592"
     CDS             complement(6538..7470)
                     /locus_tag="L3M60_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HBN6227243.1"
                     /translation="MDIQLSILLFFQNLRNPILNFIFLVLTISTEAPLLILITTIIYW
                     CINKKCGQKILFAIIGNFVVNLGIKEFVKAPRPIGIKGLESLRVSTAGGYSFPSAHTQ
                     TATTFWVSIMTIFKKRYIHVIGAVMVLGVGLSRLYLAVHWPIDVIAGWILGIFFTVIF
                     IKIFDYIDDNKNYILLLVLLIPFIIVAIFLNSSEYFEKFGIIVGFVFGYIVEDRFVKF
                     NTDNNNKKINFSSKNKGSKNIIFSRICRFLVGIVTIGMLYIGIKLFISMLIVTLNISD
                     STMSINFMNFIKNTVVVFYGVAGAPALFKVLKLN"
     gene            complement(7559..8086)
                     /gene="hpt"
                     /locus_tag="L3M60_002593"
     CDS             complement(7559..8086)
                     /gene="hpt"
                     /locus_tag="L3M60_002593"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBN6227244.1"
                     /translation="MYKVTGKMLTEEQIKEKVYELGKKIEEDFKGEDLLVVGILKGAS
                     VFVSDLIRCIDLDVNIDFMSVTSYGNSTESSGTVKILKDLDVDIEGKNVLIVEDIIDS
                     GLTLSNLVAALKTRNPKSLKLCTLLDKPQRRKANIPVDYVGFVIEDKFIVGYGIDYAE
                     KYRNLPYIGIVEDVE"
     gene            8315..9391
                     /locus_tag="L3M60_002594"
     CDS             8315..9391
                     /locus_tag="L3M60_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6227245.1"
                     /translation="MSTNHGANLYSLSSKYGFSKEEFMDFSSNINPFGTSSLAKQYIV
                     NNIDMVSMYPDPDYIDLKTSISNYCKCSMDNIVLGSGATELISSFIHTINPKQALLLS
                     PAYSEYEKELSKINCSMDKYFAKEEDNFHINLENLIKTINAKDYDLVVICNPSNPTGF
                     AFTKVEVREILKNTDSFLMIDETYVEFTDTNTYSCTQLVDDYSNLFVIRGTSKFFSTP
                     GIRLGYGLISNTNVKNEINKNIDLWNINIIASKMGEIMFSDLDFISNTISLVSAERNY
                     LLKELKNIKNLNVYDTKGNFILCKIKTKELTAKSLREKLIPQKIIIRDCCSFEGLNEY
                     FFRVCILKPNENKLLISSLKAIFK"
     gene            complement(9499..10707)
                     /locus_tag="L3M60_002595"
     CDS             complement(9499..10707)
                     /locus_tag="L3M60_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBN6227246.1"
                     /translation="MKKKIGLVPKLIIGIIVGILIGSYAPEIIVQILVTVSTLFSAFL
                     KFVIPFIIIGFVTAGIADLATGAGKLLGITTGIAYGSTLIAGLLSFVVSTLIFPNFID
                     ASVASQIGDPEAGMLAPIFTIPLEPMVDVTAAIVFAFVMGLGISALRNHGKGETLFNF
                     FQEFQGIVTKTLSTVIIPLLPLYIAGTFANITIAGEVWTILGVFWKVYLVVIPLHFVY
                     MACQFTAAGVFSGKNPVRMLKNQVPGYLTAIGTQSSAATIPVNVGCAEKNGVSKQIRE
                     FVVPLCATIHLSGSMISITGFTTAVLMMNGMPHGIGVLFPYIAMLGIAMVAAPGAPGG
                     AIMSALPFLPMVGIPSDGGLASLMIALYLTQDSFGTACNVSGDNAIAVVVDKINDGMS
                     KKGSKEKQSA"
     gene            11106..11288
                     /locus_tag="L3M60_002596"
     CDS             11106..11288
                     /locus_tag="L3M60_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227247.1"
                     /translation="MINIKPKENKHMTKTMICDYCNKVINNNSEYITFHKKNYVKTSV
                     CINCVLKLMDKTKENK"
     gene            complement(11610..11798)
                     /locus_tag="L3M60_002597"
     CDS             complement(11610..11798)
                     /locus_tag="L3M60_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6227248.1"
                     /translation="MIKMRLKGKVTVLILACLVSIGVVGCGDKENNDTTKNKAEKSIT
                     DGQENSTDTAPDVTSDSK"
     gene            12201..12518
                     /locus_tag="L3M60_002598"
     CDS             12201..12518
                     /locus_tag="L3M60_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227249.1"
                     /translation="MNFGNINLIIIGVGIIILTTIISLIKPKISFCSEKYFNKLESIY
                     GNIDRKRTVKLEVLSRYVMGLEYIVIGLFTRRLDITIIAMIIVAVITTVLYYLIRKKY
                     ITI"
     gene            complement(12579..>12824)
                     /locus_tag="L3M60_002599"
     CDS             complement(12579..>12824)
                     /locus_tag="L3M60_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6227250.1"
                     /translation="NNSVLDNLYVAKNGENKEDDLVDALSVGVLAGKTESPVIIVGDG
                     IKDVQKSLIKKKEFRYITQIGGNGNEKAFSEIENLVK"
BASE COUNT         4396 a         2056 c         1322 g         5050 t
ORIGIN      
        1 tttaaattaa atatgttaag tttttttcat aattttgtat acaatatata ataaattaag
       61 ttagactttt caaacttttt tatctatttg tttatattag tataaaaagt aaaatgatta
      121 tccttattat atgtaaattt ttatatatat aattttacca tattacatca tatttttgta
      181 agtttttatt acatttttta taatacccct ttattttata aattaatcta atcgattaaa
      241 tttatcctat agaaatttat tacatttaaa aatatgctag caaataaaat atctactagc
      301 ataaagtcta tttataatat taatcaacta ttaattctac taagtctcca tttttaacgc
      361 ctgctgcatt tccctcttcc atatcaacat gcatttctag attaaagcta tcactagctc
      421 ttactaatac attttcaaat actaatgctc ttggtccaga tgttttaact tttactattt
      481 gtttatcagt tacaccaaat ttagcagcat cttcattgct catatgtata tgtctagaag
      541 caactataac tccttctttc atttcaactt ctccagcagg acctactagt ttaacacctg
      601 gagttccagc tatatcacca gattctttta ttggtgcttt cactcctaat gggaaaccat
      661 ctgctaaaga tatttcaact tgagtatgag ctctagctgg tcctagtact ctaactcctt
      721 ttaaagttcc ttttggtcct actaaatcca ctttttcttc acaggcatat tgccctggct
      781 gagataatgg tttaaattta gttaactcat gacctgcacc aaataatacg tctatatcag
      841 cttgactcaa atgaacatgt ttattggata atgctattgg taatttcatt gaaatttcct
      901 ccttttaatt atttaaatta ttgcaataca attatacata atattaccta caaaatcaaa
      961 ttatattgaa tcaatatatc tctttaaatt tttatatatt atatctttaa attcttgatt
     1021 gctcatataa gatacattgt ctgtatcgtt taaatatata ttgtgtaaaa gtttagtatt
     1081 tatactctta aagaaaaaat ccataacaat ctgtccacct ttaaactgtg aatcatatga
     1141 tttactaccc cctactgcaa tatacattcc tatccgtttc ttgtttctat caattgaggg
     1201 cttatttaat atatatttac ttgcatatat agcttgtgtt ctatcaacaa ctgtctttaa
     1261 tttagctgat attgaatcaa aatgtacagg acttattact atagttcctt ttgaatcatc
     1321 aaacatttta tataatggct tcatatcatc attaaactta caataccctg ttttttcaca
     1381 aaatccacat gctgtacaat attcaatatc catatcgtat atattaaata ttttacagct
     1441 tagtccatac tcttctagca tagcttttac ttcatttgct ataaaactgc aatttttatt
     1501 aattcttggg cttccattta ttatcataat ctctggactt ttaagtcctt ttttatcagt
     1561 tgaatccata atcaatctac ctatccttta acataactat taaaatatca tcatcataat
     1621 tatcttttga tgagaaattc tttaaatcca actttagatt ctctactatt aactgttgat
     1681 ttaatctaaa gttttctttt agaaagtttt ctaatctgtt tataccatac tcttcttttg
     1741 atgaattttt tatctctaaa attccatcag tatacataca taccattgca taatcttcca
     1801 ttgaaatagt attattttca taactagcat cttccattat tccaataggt ataccctttt
     1861 tacaatttaa attattttct attgtaccat ccttctttac gactacagga ctatagtgac
     1921 ctgcattaga cactgtaatc atgttttctt tagtatctat aatacctatc aaacaagttg
     1981 taaatactcc catcttgtca aattcatcat ataacatcct atttagattt gtcattattt
     2041 ctcttggtgt attgtattga ttacataata ccttaaaagc accttttatc attgcaacaa
     2101 cataatttga taccattcca tgtcccatta catcagcaac aatataaact atatgttcgt
     2161 catctacttt tatagcataa taaaaatctc cacctactac tcttgctggt ttatgataaa
     2221 aataaagttc tttctcatca ttacaatcca ttttaccttt atccataata agctgttgct
     2281 gtttatttaa tatgttcagc tcattttcaa gaactttgtg ttttacttcc ttttcattat
     2341 gttcatataa ttgcatacca acagaaactt gtttggacag tatactaaga atattcaaat
     2401 catcactagt atagttttta ctatttgcac aaactataca tcctatagtc tcatttttat
     2461 cctggagttt gtaatatata taactttcca aatttattcc tttttttgag catggtttta
     2521 aattatttag ataccctccc aatgattctt tctttaaaaa cgtatcaatc ataggatata
     2581 aatttgtttt ctttttttgc tcaacattat aacagctaac tatagcatcc ttattatgat
     2641 gactatttat aattattaaa gcttctttac tattagtaat attgcatgct tgtttactta
     2701 aattttctaa aaagccatca atactcttat taaggtatag ttttttagta gcttcattta
     2761 tggcacttat tgagcctctt agtttatcaa ttaaaatttg tacattatta tttttattag
     2821 ttatttctat tgataaactt aagagtgatg caacagatga tataaagtct atatcttgtt
     2881 ctgttaaata atcatcctct tttaaaaaac aagtcataaa tccaacaact acattattta
     2941 ttataaaagg aaaaactatt ctcccaatat aaccttcagc ttttgctaaa tctcgttcac
     3001 cttctgctct ggaatcttca aatacatttt ccacatatat tatctttttt tcatttacag
     3061 cctcatgtat atactctgga tacgtgtcaa aatcaatttt tgcaccttta agactattta
     3121 ttggaaaaat ttgaggtaca tattctaagg tttgtgaaca tactaagtag gcatatttag
     3181 aatcattttt ataaaataga ttcacacatg cctttgtagg gtgaacaact tctaacattt
     3241 tttctattat gtcatctttt atattaaaaa aatctgtaga ttcagttacc atagcggata
     3301 tctccactaa attcttcatt ttataactgc cattagtcat actcaatttc tccccctata
     3361 tattaaacta tttattagta ttttaacata cattctttat agtaaaaaca aaaacaacga
     3421 caaaactagc aactaatatc cagttaaatc ctgttaataa tcctataaga agtaatgtaa
     3481 ttaatgcatt tagacctgtt ctaccttttt catccataaa agttatttta gagatgtcga
     3541 tattattttt cacattattt atatgttctt ttacttgttc ttccatattt cttttatata
     3601 gtatataaac tacaccaact aaaaatactc ctatacctag aaccatgttt aaattaaaag
     3661 ctaccataag caatattgaa ccaaaagcca actttaaaag cttaaaaaat atactatcta
     3721 tctttaaggt tatatttctg atttctttac attcgtctct taaagaatat ccacttgaat
     3781 ctaagttatt aatctttgtt tttacaattt cagaataaaa aagttttatt attccattta
     3841 acattttcat cactcctaaa tattttacat atatttgtaa gttttacaat tataatattt
     3901 tttatacttg gtaataataa aaaataacaa atattttatg atttagtatt tattgtaaat
     3961 aaattattga agctttatta gttaagtata gatttttaat aactattttt ataattagaa
     4021 ggaagtggtt ttgtgcaaat agaacaactt gatttagaaa cgagaaataa aatatactgc
     4081 tatacaaaaa aaattcttag aaagtatcaa aaaggtataa catctggaaa attaacagcc
     4141 gataaatttg cagataatat attgtctcaa gattttatca gtagtatact tgatgaaaaa
     4201 atagtcaatg agaccaactt taagatatct tatagaaact atatagaaac tttgataaat
     4261 attcaaaatg aaaatttatc taactttaaa aaaaaatcta ctaaaactgc aaagtgcttc
     4321 aatatctctc aaataaccca attaaaaaac ttactaaaca atactggtta taatttatta
     4381 attccatata aatacttaac ttctcgtgat atagaaggaa tcgttactct tattaatact
     4441 gggtctattg aacttggaaa tgagagaatc tataattata tatccaaagc ataataattt
     4501 aaacctatta taaaaaggca atttatttaa taaaaaatat ttttaatatt aaataaattg
     4561 cctttttaat tttggactaa atactgctat ccaagtgctt tttctagttt agataaatgt
     4621 tgtattggat aatattttag tagctcttta aactgttcta ctgatagacc ttctgttgtt
     4681 gtatatattc ttattttttc atctaagtca ctattaataa atttgttttt taaatcctca
     4741 aaatttacac tactcatttt acatcactcc ttatgtaaca tattcatttg tattatttta
     4801 tccaaataca taaaatttat tacataaaaa tatatctatt taataattta ataaaaaaaa
     4861 gctatctcaa tttagaaatt actttctata tttgagatag ctttagctaa actaatagcc
     4921 aatagttata aataattatt tataactatc tgttgctttt tccagccatt tgttgttcag
     4981 ccatttcaac taatttttta gtcatgtatc cacctacata accattttct cttgcagtaa
     5041 ggtttccttt atcaacttgt tgataattac tcataccaag ttcattagct atttctaatt
     5101 tcatttggtt taaagcctct tttgctcctg gtactactgt tctgttgttg ttattgttac
     5161 ttgccatgtt gattaccttc cttctgttta atttattttg tgttgctgat aatattatgt
     5221 gattttaaac atttttaatt caggtaattt ttagcaatta aaacctgcta aatatccagt
     5281 agaaaaagct atttgcaaat tgaatccacc tgtatatgca tctatatcta ttacttctcc
     5341 agcaaaaaat aaaccttcta ctaattttga ttccattgta cttgaattta tttcatttac
     5401 ttttacacca ccagaagtta ttatagcttc atcaataggt ctgtatctct ttactgtaaa
     5461 ctttaaattt tttaataaat ggactaagtt ttttctctgc tctctagaaa tttggtgaac
     5521 aactgtatct ggattaattt cactcaaatt tactattaca ggtattagtt ttttaggaag
     5581 taaatcatct aatgcttttt caaatttttt atttgtatac ttctgaaaat ctttttgaat
     5641 tcttttatcc agtttttcct catctaatgc aggttttaaa tctaatatta tagtgtaatt
     5701 ttctttcctt gtatctttca ttctacaact tgcagacttg attataggtc catctaaacc
     5761 aaatttagta aattcaagtt ctccaaaatc actataaact ttcttttgtt tgctattaaa
     5821 tactcttatt tcaacatttc tcaatgataa tttttctaaa tctcttgtaa aactttcttg
     5881 tacttctata ccaattaaag atggttttgt atccataatt gtatgtcctt gtgaaattgc
     5941 aaatttatat ccatcaccag tagaaccagt aagtggataa cttaatccac cagttgctac
     6001 tacaactgaa tcacatttaa tttctttctt attgtttaaa attacttttt caattttatt
     6061 attcttagat attatttttt ctactttcga atttaataat atatttactt tcttactttt
     6121 aagttgtctt tcaagagcgt ttacaatatc aaatgcttta tcgctctctg gaaaaaccct
     6181 ttttcctctt tctgttttag tttttactcc aagattatta aacatactta ttacatcttc
     6241 atttgtaaaa gtataaaaag cactataaag aaattttcca tttgtaggta cattttctat
     6301 tagttcttct atttcacagg cgttggtaat attacatcta ccttttcctg ttattgcaag
     6361 tttttttcct aatttatgat ttttctcaac taacgttaca tcacagcctc tctcacatgc
     6421 agttgatgcg gctatcattc ctgctgggcc accacctatt attactacct tcttcaaata
     6481 atcactcttt tctaccctta atttttttaa tttaaactat tgattatata cctcaaatca
     6541 atttaatttt aaaactttaa ataatgctgg agctcctgct actccataaa aaacaactac
     6601 tgtattctta ataaaattca taaaatttat actcatagta gagtcactta tatttaaagt
     6661 aactattaac attgaaataa acaacttaat gccaatatat aacattccta ttgttactat
     6721 cccaaccaaa aacctacata ttcttgaaaa aattatattt ttactcccct tatttttact
     6781 gctaaaattt atttttttgt tattattatc tgtattaaat ttaacaaatc tatcctcaac
     6841 tatatatcca aatacaaacc ctactataat accaaatttt tcaaaatatt cagagctatt
     6901 tagaaatata gctactatta taaacggtat aagtaaaact aaaagtaaaa tataattttt
     6961 gttatcatca atatagtcaa atatttttat aaatattact gtaaaaaata tacctaatat
     7021 ccaaccagct atcacatcta ttggccagtg aacagctaaa tacaacctag acaaccctac
     7081 tcctaaaacc atcactgcac ctattacatg gatatatctc tttttaaata tagtcataat
     7141 actaacccaa aacgtggttg cagtttgagt atgagcactt ggaaaagaat aaccgccagc
     7201 agtagaaact ctcaatgact caagaccttt tattcctatt ggtctaggag cttttacaaa
     7261 ctcttttatg cctaaattta caacaaaatt tccaattata gcaaacagta ttttctgtcc
     7321 acatttttta tttatacacc agtatataat tgttgtaatt aatattaata acggtgcttc
     7381 tgtgcttatc gtaagtacaa gaaatataaa gttcaaaatt ggatttctta aattttggaa
     7441 aaataataat atacttaatt gaatatccat atagatgtct cctttactaa aattttatat
     7501 aattaactaa aaagcactaa ctcccttaga aattagtgct tttgattcac atttaaatct
     7561 attctacgtc ctcaactata cctatgtaag gtaaatttct atacttttca gcataatcta
     7621 ttccataacc aacaataaat ttgtcttcta taacaaatcc aacataatct acaggtatat
     7681 tagcttttct tctttgaggt ttgtcaagta gagtacacag ttttaaagat tttggatttc
     7741 tagtttttaa agctgccact agattactta atgttaaacc agaatctatt atatcctcta
     7801 caatcaacac attctttcct tcaatatcta catctaaatc ttttaatatt ttaactgttc
     7861 cagaagactc tgttgaattg ccataacttg taacactcat aaaatctata tttacatcca
     7921 aatctataca tcttattaaa tctgatacaa atacactagc tcctttaagt atacctacca
     7981 ctagtaaatc ttcgcctttg aagtcttctt ctatcttctt tcctagttca taaactttct
     8041 cttttatttg ttcttctgtc aacatttttc ctgtaacttt gtacattttg tatcctcccg
     8101 ctttccttct attaacttat atattatagc ataccaaaat atcaattaaa atctttttca
     8161 gcatttttaa acctaaatta acagtttatc catacttttt tgcttttttc attaaaaatt
     8221 tggaataacg attcctttgt attataatct atatgtacat atattaatca gttatttttc
     8281 tcgattatat aattttattt ttggaggtac ttttatgagc acaaatcatg gagctaattt
     8341 atatagttta tctagtaagt atggcttttc aaaagaagaa tttatggact ttagctcaaa
     8401 tataaatcct tttggtacat caagtttagc caaacaatac attgtaaaca atatagatat
     8461 ggtatctatg tatcctgacc ctgattacat agacttaaaa acttctatat ccaattattg
     8521 caaatgttct atggataata tagtacttgg cagtggagct acagaactaa tttcatcatt
     8581 tatacataca attaatccta aacaagctct cttactctcc ccagcttatt cagagtatga
     8641 aaaagagtta tctaaaatta attgttctat ggacaaatac ttcgccaaag aagaagataa
     8701 ttttcatata aatttagaaa atcttataaa aactatcaat gctaaagatt atgatttggt
     8761 agtgatttgt aatcctagta atccaactgg atttgcattt actaaagtag aagtgagaga
     8821 aattttaaaa aatacagact catttttaat gattgatgag acttatgtag agtttactga
     8881 tacaaataca tactcttgca ctcaattagt tgatgattat tctaatttat tcgttataag
     8941 aggtacatca aaattttttt caacgcctgg cataaggctt ggatatggtc ttatatctaa
     9001 cactaatgtt aaaaatgaaa taaataaaaa tatagattta tggaatataa acataatagc
     9061 ttctaaaatg ggagaaatca tgtttagtga tttagatttt atatcaaata ctatttcttt
     9121 agtaagtgct gaaagaaact atttacttaa agagcttaaa aatataaaaa atttaaatgt
     9181 gtatgatact aaaggtaatt tcatattgtg taaaataaag acaaaggaat tgactgctaa
     9241 atctcttcgt gagaaactaa ttcctcaaaa aataataata agagattgtt gttcatttga
     9301 aggtctaaat gaatatttct ttagggtatg tatattaaaa cctaatgaaa ataaattatt
     9361 aatatcatct cttaaagcaa tttttaaata aaaaattact ttatcttata atttgtacaa
     9421 tcatactttt atcatttcaa caatgtaaaa gaggcttatc tctattttga gatagcctca
     9481 tttttatcta tttacaaact aagcactttg cttttctttg cttccttttt tactcattcc
     9541 atcatttatt ttatctacta ctactgctat tgcattatct cctgatacat tacatgctgt
     9601 accaaaacta tcttgtgtta agtataaagc tatcattaaa cttgcaagtc caccatctga
     9661 tggtattcca accattggta gaaaaggaag agcactcatt atagctccac ctggcgctcc
     9721 tggtgctgca accattgcta tacctaacat tgcaatataa gggaataata cacctattcc
     9781 atgtggcata ccgttcatca ttaaaactgc tgttgtaaat cctgttattg atatcatact
     9841 acctgataaa tgtattgttg cacaaagagg tacaacaaat tctcttattt gcttacttac
     9901 tccatttttt tctgcacaac caacattaac tggtatagtt gctgctgaag attgagtccc
     9961 tatagctgtt aaatatccag gtacttgatt ttttaacatt cttactggat tttttcctga
    10021 aaatacgcct gctgctgtga attgacatgc catatataca aaatgtaaag ggattactac
    10081 taaatagact ttccaaaata ctcctaatat tgtccatact tctcctgcta tagttatatt
    10141 tgcaaaagtt cctgctatgt ataaaggtaa taaaggtata attactgttg acaacgtttt
    10201 cgttacaatt ccttgaaatt cttggaagaa attaaataaa gtctctcctt ttccgtgatt
    10261 tcttaaagct gatattccaa gtcccattac aaatgcaaat acgattgcag ctgttacatc
    10321 taccataggc tctaatggaa ttgtaaatat tggagctaac attcctgcct ctgggtctcc
    10381 aatttgactt gcaacactcg catcaataaa atttgggaag attaatgtag atactacaaa
    10441 tgataataat cctgctatta aagtagaacc atatgctatt cccgtagtta tccccaataa
    10501 ctttccggct cctgttgcta aatcagctat acctgctgtt acaaaaccta taattataaa
    10561 tggtataaca aattttagga atgctgagaa taaagtacta actgttacta atatttgtac
    10621 gattatttct ggtgcataac ttccgattaa aataccgact atgataccga tgattagttt
    10681 gggcactaaa cctatttttt tcttcataat atttaccctc cataactttt ttaaattaag
    10741 cattcttgta cattgaatac ttagttaaat attggatatc cacttattat gataatagag
    10801 ttttacctct ccgtcaacaa tttaaccatt ttttgttaat aaaaaaaaga ataacatata
    10861 taaagtgttc cttttattta ttttatttta tatatatgtc tattttcaat attttataat
    10921 gttgatactc tttatttttt taactttcgt cagttgaatg tcagtaaaat attttatcca
    10981 catatttcat atagttttcc cacaacttat ccccgatatt atttataaac aaaaagatag
    11041 atactagtaa ctctgtttta actagtatct attaaggtat acaccaaata tattagaact
    11101 taattatgat aaatataaaa ccaaaggaga ataaacatat gactaaaact atgatatgtg
    11161 attattgcaa taaagtaata aataataata gtgaatacat tacttttcat aagaaaaact
    11221 atgtaaaaac tagtgtatgt attaattgtg tgttaaaatt aatggacaaa actaaagaaa
    11281 ataagtaata aacaatatta tttgagaaag tgaagaaaac actctaccat aaggaaaatg
    11341 ctctatctaa tataaatatg gtaaaaaagt aaagatgtat tgggcgtaca tctttacttt
    11401 tttaccatat cacattacta ccctaataaa aacagcttta tgtacaaaaa caccatttaa
    11461 aaaggatgtt ttcttggata atattgtttt aactagtaaa gatatattga ggggttatct
    11521 ttaccttttt tattatatta taaatttcct aaaagtaaaa ttataattaa aatatgtata
    11581 aattacaata attagtaaac tatcttttat tatttagaat cacttgttac atctggtgct
    11641 gtatcagttg aattttcctg tccatcagta atactttttt ctgctttatt tttagttgta
    11701 tcattgtttt ctttatcacc acaacctaca accccaatag atactaaaca agcaagtatt
    11761 aggacagtaa ctttcccttt taatctcatc tttatcatcc tcctagataa atattaattc
    11821 actatttaaa attatagcaa agtaatgtta atcttatgtt tattgtaaaa aatatgtaat
    11881 ttatatgctt agaatattat attttctaac aaaatgtaat atagtagaaa atacattaat
    11941 gaatttcaaa aaataagcac tcatataaag agtactttag tgtatattaa tgaatgttta
    12001 taatctaatt taacatgtct tatgttttag tatgataatt ttcgttcgct ttattattac
    12061 aacttatgtt ctagttttat gtatcctgct aatatttgta atattttaga tacgctttat
    12121 gcttatgttg ctgatatact ttgattaaga aaaaaaatct aatataaact ggtgattata
    12181 ttataaagga ggtatctact atgaattttg gaaatattaa tcttataatt attggagtag
    12241 gaattataat attaactaca ataatatctt taattaaacc taaaattagc ttttgtagtg
    12301 aaaaatattt taataaatta gaatcaatat atggaaatat agatagaaaa agaactgtta
    12361 aactggaagt gctttctcga tatgtaatgg gtcttgaata tatagtaata ggtttattca
    12421 caagaagatt agatattaca ataatagcaa tgataatagt agccgttata acaacagtat
    12481 tatattatct aattagaaag aaatatataa ctatataaaa tctaataaaa ttaatactag
    12541 aagaaggtag caactagtta cggttgctac cttctaatct attttactaa attttctatt
    12601 tcactaaatg ctttttcatt tccatttcca ccaatttgag ttatatatct aaattctttc
    12661 ttctttatta aacttttctg aacatctttt attccatctc caactattat tactggtgac
    12721 tctgtcttac ctgcaagaac acctactgat aatgcatcta ctaaatcatc ttccttattt
    12781 tctccatttt tcgcaacata taaattatct aaaacgctat tatt
//
LOCUS       DAFNNB010000112        12794 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0113, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000112 DAFNNB010000000
VERSION     DAFNNB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12794
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0113"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(4..2232)
                     /locus_tag="L3M60_002600"
     CDS             complement(4..2232)
                     /locus_tag="L3M60_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HBN6227251.1"
                     /translation="MKLVLAEKPSVAKTIASFLGAKTRQDGYFEGNDYIITYAVGHLV
                     SLYDMKDYDKDKYSGSWKMNNFPFIPEDKFKFKIDSSKTKQFNTVKKLLNRKDVEYVI
                     NATDNDREGELIAFLIFLLAKNKKPVKRILVNEWTPEDITRGIKNLKDEDEMRNLQAA
                     GYTRLITDWLIGINFTSVATLKYGNGKLLNIGRVILPTVKLVYDRDMEILNFVPKTYY
                     EIEGHFKAETGEYKGKYIKGKESKFDTLEDANKIIASIASKTGKILDKKVTMSKEYAP
                     KLFSLTSLQGYITSKYSNFTSDKVLNVCQSLYEGNGKGGYITYPRTDSIFLEESLASK
                     ASQTLDKLKVGLEYENKIKFSKTKRVFDSSKVDSHSAIIPTYIIPKNLSKDEQLVYDA
                     IKDRFIANFMPPAEYENTEIKTEVDNCTFLTKGKVLKSKGYLEVYNKEEKNDLLPLVN
                     KDDVVDVLEIKPLTKQTTPPKPYTEDTLLKAMKNCGKNVPEEDTTVLSGYSIGTSATR
                     ADVLKKINQVGYVKKKGKSYSITDLGKNLVEIFPVKDLFDVDYTGKLEKSLSDIQKGK
                     YTRKEYLTNIISFIYQNVNIIKQDASKNINTEEYTFDSKTKKFTKTKEKKQKKDTTNA
                     EKEVAISKDSNNKKESKNGDENQPLGKCPVCQGDVLEFDKGFACKNHKECKFVIWKND
                     KYLSLYKKKVNKTMVKNILKKGETKVKSLTAKNGNKFDALLKYNKNPDTGYFNWKIEF
                     DN"
     gene            2345..2776
                     /gene="dut"
                     /locus_tag="L3M60_002601"
     CDS             2345..2776
                     /gene="dut"
                     /locus_tag="L3M60_002601"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBN6227252.1"
                     /translation="MYTLKVKKINDDAIIPNFAHKGDAGMDLYSIEEVVIPSGETKLI
                     KTGICIELPTMTEAQVRPRSGLALKHSVTVLNTPGTIDEGYRGELKIILINHGKNDFK
                     VEKHMKIAQMIIKPIYDINIEEVEELSDSERGKGGFGSTGF"
     gene            3036..4226
                     /locus_tag="L3M60_002602"
     CDS             3036..4226
                     /locus_tag="L3M60_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBN6227253.1"
                     /translation="MNFNQKRIAASVMATAIIIPTMGNLAYANESEVESVSIESRTIT
                     GNAVNFRKGPGTNHESMGKLYKGDKVEYVGKDGSWVKVKYNGNTGYVHGNYVAINSLG
                     SSNESSDTSVKSTKVVTAKGLNFRTGPSTSSSKISTLGYGTEVGYISESNGWSKISSN
                     GRVGYVSSKYLGTSVNDSTSENTGNSSNDIVKGTKVVTAKSLNLRTGPGTSHSKIATL
                     SYGTEVGRISENGGWTKVSYGNQTGYVSSQYLAEKGSVDTSIPSYSTNSPSQGAESVI
                     SFAKTLLGKPYVWGAEGPNSFDCSGFTQYVMKKSAGVSIPRVSRDQSKYGTYVNRGDL
                     RSGDLIFFDTQGSNNGSVSHVGIYIGNGDMIHASSGSSKKVTISNINSSYYSSRYVNA
                     RRVL"
     gene            complement(4464..5036)
                     /locus_tag="L3M60_002603"
     CDS             complement(4464..5036)
                     /locus_tag="L3M60_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HBN6227254.1"
                     /translation="MWIYQKTIQHPVNIKTCDPRMAKFLITQFGGPNGELAASLRYLS
                     QRYTMPTGNMRALLTDIGTEELAHVELICTMVYQLTSDASPEELKAAGLGSNYAQNGY
                     GIYPTDSNGVPFDVRPIAVMSNPVTDLHEDMAAEQKALATYYQLINLTDDVDVIDVLK
                     FLGQREIIHYQRFGEALMDAYELEESQKMF"
     gene            complement(5065..5331)
                     /locus_tag="L3M60_002604"
     CDS             complement(5065..5331)
                     /locus_tag="L3M60_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein CotJB"
                     /protein_id="HBN6227255.1"
                     /translation="MKDSSRAELMRKIQETSFACVDMNLYLDNHPDDKNAINTYNSLC
                     NQFAQARYAYENKYGPLTNFGYAPSRCPWQWADQPWPWDREFNY"
     gene            complement(5331..5540)
                     /locus_tag="L3M60_002605"
     CDS             complement(5331..5540)
                     /locus_tag="L3M60_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat associated protein CotJA"
                     /protein_id="HBN6227256.1"
                     /translation="MENKTRDIKKNCGPSLARPYINNQIFTEMYCLSDALKFGTIFPE
                     LNLLESEMYNKELYAKPKKYRGGRR"
     gene            5809..6669
                     /locus_tag="L3M60_002606"
     CDS             5809..6669
                     /locus_tag="L3M60_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBN6227257.1"
                     /translation="MSELSGKGCEIIVPFEERLPVRDIEKSIIKKYRKNLWSKFMKAI
                     RDYKLVEEGDKIAVAISGGKDSILMAKMFQELKKHGQVNFDVEFIAMDPGYHANIRQL
                     LIDNCEYLNIPIHLFDSRIFEIADEIAKDYPCYMCARMRRGALYSKAEELGCNKLALG
                     HHYDDVIETTMLNLLCAGNFKTMLPKLNSTNFEGIKIIRPLYYIREEHIIRFIQNSGI
                     WPLNCACMVAAKKTGNKRYEIKDLIKSLESNFKNVEKSIFKAAENVNLDSVLGWQKDG
                     EKHSFLENFE"
     gene            6717..7745
                     /locus_tag="L3M60_002607"
     CDS             6717..7745
                     /locus_tag="L3M60_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBN6227258.1"
                     /translation="MKRVYIDFEMNMPNSKSKRAVFNSDIIAIGAVMYDEKTKNIDKF
                     KSLIRPVSNEELYPHIQELTHISSEELKSAPSYEEVMRKFKKWLGIFSDIKGIYTFGN
                     LDLTCFNNTDMRSAKKNNHPRFVNNIRNLFVDIKEKYINCGMRCMNYISLKNLLEFVN
                     LEFSGDAHDPLNDAYNLFILDEAITNNIDIQNLLIIRDIIRAPFNDINSNLDNCFDKF
                     KESLYKKEENYNIVDFSVEIIKTVRMYLLTIIDVNIQNLEVMKDISKKMDTIDKLKDI
                     EEGYFYLLEDVYFDMKDILEDLMLYRMHEDEYKHEIENIIKMLDEDLDNEKIYINKCN
                     DLNVVNKI"
     gene            8109..8615
                     /locus_tag="L3M60_002608"
     CDS             8109..8615
                     /locus_tag="L3M60_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HBN6227259.1"
                     /translation="MGFREVKIEELQFNPFTKIGKEWLLITAGDSEKFNTMTASWGGV
                     GVYWGKNVVTTYIRPQRYTKEFVDSNDTFTVAFFDETYREALNICGTISGRDINKIEK
                     AGLTPYFVDDTVAFEEANMIIVCKKLYHGNMPPENFDAKENDKKWYPEKDYHTMYISE
                     IIKVLVKE"
     gene            9028..9696
                     /locus_tag="L3M60_002609"
     CDS             9028..9696
                     /locus_tag="L3M60_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227260.1"
                     /translation="MENITSYFTTILCVFICLSSVFIFTQLARVFINKKKINQKIKSR
                     KGFRYDRDFIEARREEIHIKDNNNNKNKSNNKKLKGEKVFKYDNGDLYKGEFVDGKKN
                     GFGIYIFSSKEKYEGLWKDDKMHGIGKYTYKDGSIYTGEFKYGLKNGLGKLTYPNNDI
                     YKGYFLDNKKNGKGILYKNDGNKQAGIWEDDEQCKSLDFKDLSNNEISKVSQNRLNNN
                     KLRV"
     gene            9855..10361
                     /locus_tag="L3M60_002610"
     CDS             9855..10361
                     /locus_tag="L3M60_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HBN6227261.1"
                     /translation="MKEYVVVDLETTGLDPYKGCEIIEIGITEIKNEQIVKNYSRLIK
                     PKGIISSFITELTHISNEMVENEEPLELVLPRFRKYIGDRTIIAHNAKFDLKFLNYYL
                     RMLNLEPINNYICTVELLKKCKSYKGKNKKLETACAYYNIENINAHRADSDTLATAKL
                     FLKIKDEY"
     gene            10489..11505
                     /locus_tag="L3M60_002611"
     CDS             10489..11505
                     /locus_tag="L3M60_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HBN6227262.1"
                     /translation="MGYYNDNDNNKIRRKKISNSKDSNKKSKHSYTSANKKNKSSKTS
                     SSKRMSEKVDSQNLIKRDRDSFTRDNVRPLNTDYNQRERRRVEYKQREKKLKSKKRKK
                     QKRIRIVRALLSIMLVALIIFSVAGAIFTVSAIKGSPEVTKKLIRSKYISSEVVSIKQ
                     MPDDLKHAIVSIEDERFYKHKGVDVISLVRSVLNNVFTDTTQGGSTIDMQVSKNLLTS
                     DDKTMKRKIRDMYNAKQMNKIMTKDEILEAYLNNMYLGKNAYGAAKGAEVYFGKKVNE
                     LNLAECAMLAGMTNNPARYIDHGEAKKRQLIVLYKMHQLGYITDKEYRIAKADDTPFK
                     SEID"
     gene            11861..12565
                     /locus_tag="L3M60_002612"
     CDS             11861..12565
                     /locus_tag="L3M60_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B3/4 domain-containing protein"
                     /protein_id="HBN6227263.1"
                     /translation="MKKFVIEDTFWNLFPNAKIGVIICNDIDNSIKDEDYKKIILQGE
                     EEALKYLEDSEFSKNQVIKVWREAFKKFKTKKGARSSIEALLKRVHNGNNIGTINPLV
                     DIYNFISLKYALPCGGEDIDKFIGDIRLTRAIGNEEFIPLGTDENLFPYEGEIIYKDD
                     GGAICRCWNWREAVRTMLTEDTNNAFLCIELVDESRFKEFENALSELAKIVQEKLGGK
                     CKISILDIDNKEVSVD"
BASE COUNT         4962 a         1386 c         2080 g         4366 t
ORIGIN      
        1 tatttagtta tcaaactcta ttttccaatt gaaatagcca gtatctggat ttttattata
       61 ctttaataaa gcatcaaatt tattaccatt tttagcagtt aaagatttta ctttagtttc
      121 accttttttt aaaatatttt tgaccattgt tttgttaact ttcttcttat aaaggcttaa
      181 atacttgtca tttttccaaa taacaaattt acattcttta tgatttttac aagcaaagcc
      241 tttgtcaaat tccaatacat caccttgaca aacagggcat ttaccaagag gttggttttc
      301 atcaccattt ttagattctt ttttgttgtt agaatctttt gatatagcaa cttctttttc
      361 agcattagtt gtatcttttt tctgtttttt ctcttttgtt ttagtaaatt ttttagtttt
      421 tgaatcgaag gtatattctt ctgtgtttat attttttgaa gcatcttgtt ttataatatt
      481 gacattttga tatataaaac taatgatatt agtaaggtat tcttttcttg tatactttcc
      541 tttttgaata tcactcaaac ttttttcaag tttcccggta taatctacat caaataagtc
      601 tttaacagga aaaatttcaa ctaaattttt cccaaggtct gttatagaat aagattttcc
      661 ttttttcttt acatagccta cttgatttat cttctttaat acatcagcac gtgtagctga
      721 tgttccaatc gaatatccgg acaacactgt tgtgtcttct tcaggcacat ttttaccaca
      781 atttttcata gcctttaaaa gagtgtcctc agtatacggt ttaggtggag tagtttgctt
      841 agttaatggt tttatttcta acacatctac aacatcatct ttatttacaa gtggaagtaa
      901 atcgtttttt tcttccttat tatatacttc aagatatcct tttgatttaa gcaccttacc
      961 ttttgttaag aaagtacaat tatctacttc agtcttaatt tctgtattct catattcagc
     1021 aggaggcata aagtttgcta tgaatctatc ttttattgca tcataaacta attgttcatc
     1081 cttagataag ttttttggaa ttatataagt tggaattata gcactatgag agtctacctt
     1141 agaagaatca aagactcttt tagttttaga aaatttaatt ttattttcat attcaagacc
     1201 aacctttaat ttatctagag tttgagaagc ttttgaagcc aaactttctt ctaagaaaat
     1261 agaatcagtt ctagggtaag ttatgtatcc acccttacca ttaccctcat aaagagattg
     1321 acagacattt aaaaccttat cagatgtaaa attggaatac ttagaagtta tatatccttg
     1381 aagtgatgtt aaactaaaaa gttttggtgc atattctttt gacatagtaa cttttttatc
     1441 tagtatttta ccagttttag atgcaattga agctataatt ttatttgcat cctcaagtgt
     1501 atcaaattta ctttctttgc ccttaatata tttacctttg tattcaccag tttcggcttt
     1561 aaaatgaccc tctatttcat aataagtttt aggaacaaag tttagtattt ccatatccct
     1621 atcatagact aatttaactg ttggaagaat taccctacct atgtttaata gtttcccatt
     1681 tccatatttc agtgtagcaa cagaagtgaa atttattcca atcaaccaat cagtaataag
     1741 tcttgtatat ccagcagctt gaagatttct catctcatct tcgtccttta agttttttat
     1801 acctcttgta atgtcttctg gtgtccattc atttactaag attcttttta ctggtttttt
     1861 attttttgca agtaaaaata ttaaaaatgc tataagttct ccttctcggt cattatcagt
     1921 agcatttatt acatattcca catcttttct attcaaaagc ttttttacag tgttgaattg
     1981 tttggtttta gaactatcaa ttttaaattt aaatttatcc tcaggaataa atgggaaatt
     2041 gttcattttc caagagccag agtatttatc tttatcataa tccttcatat catataatga
     2101 tactaagtgt ccaactgcat aagtaattat ataatcattt ccttcaaagt aaccatcttg
     2161 tcttgtcttt gcacctaaaa atgatgctat agtttttgcc acagatggtt tctcagcaag
     2221 tacaagtttc ataaaaaatc ctcctttaaa acactaataa ttatattacc acaaaaaaat
     2281 aaaaaacttc aagaggtcga aatttaagtt ataatattta tagaaagttg aaaggagaaa
     2341 taagatgtac actttaaaag taaaaaaaat aaatgatgat gctataatac ctaatttcgc
     2401 tcataaaggt gatgccggta tggatttgta ttctatagag gaggttgtaa taccttctgg
     2461 agagactaaa cttatcaaaa ctgggatatg tatagaactt cctactatga cagaagcaca
     2521 ggtaagaccg agaagtgggc ttgccttaaa gcattctgtt actgtattaa atacaccagg
     2581 aactattgat gaaggatata gaggagagct taaaattata ttaataaatc atggtaaaaa
     2641 tgattttaag gttgaaaaac atatgaaaat tgctcaaatg ataataaaac ctatctatga
     2701 tattaacata gaagaagtag aagaattaag tgactctgaa agaggaaaag gtgggtttgg
     2761 ttcaactgga ttttaaaaag ttacaaaaaa gttaaaaaat atagctattt taaaaaaaag
     2821 caaaatatgg ctatattgct aaattacagg ctgttaagga ttaaaattta tacatggtaa
     2881 tttatggtaa acaaatatta ctatagacaa atagttacaa atttgtaata atattaatat
     2941 aaaagaaata attgatattt taagagtgtc aattatttct tttatatttt gaaatgaaat
     3001 aaagctagta gaaaagagga gatgagtgga aagttatgaa ttttaatcaa aagagaatag
     3061 cagctagtgt aatggcaact gcgataataa tacctactat ggggaattta gcatatgcta
     3121 atgagtctga agtagaaagt gtgagtatag aaagcagaac aataacaggg aatgcagtaa
     3181 attttagaaa aggaccaggt acaaaccatg aatcaatggg gaaactttac aaaggtgata
     3241 aagttgagta tgtaggaaaa gatggttctt gggtaaaagt taaatataat ggaaacacag
     3301 gttatgtgca tggaaattat gtagcaataa atagccttgg aagtagtaat gaaagtagtg
     3361 atacttcagt taagtcgaca aaagttgtaa cagcaaaagg cttaaatttt agaactggac
     3421 caagcacaag tagttctaaa atatcaacac taggatacgg gacggaagta ggatacatat
     3481 ctgagtcaaa tggatggtca aaaataagtt ctaatggtag agtgggttat gtttcaagta
     3541 aatatttagg aactagtgta aatgactcta caagtgaaaa tactggaaac agctcaaatg
     3601 acattgtaaa aggtacaaag gtggtaacag caaagtcatt gaaccttaga accggaccag
     3661 gtacaagtca ttctaaaata gctacattaa gttatggtac tgaggttggt cgtatttctg
     3721 aaaatggagg gtggacaaaa gtaagttatg gcaatcaaac aggatatgta tcaagtcaat
     3781 atcttgctga aaaaggttct gttgatactt caattccatc ttattcgact aattctccaa
     3841 gccaaggagc tgaaagtgta ataagttttg caaagacact acttggaaaa ccatatgtgt
     3901 ggggagcaga aggtccaaat agttttgact gttctggatt cacacaatat gttatgaaaa
     3961 agtctgcagg agttagtata ccgagagtat ctagagacca aagcaagtat ggaacatatg
     4021 taaatagagg agaccttaga agtggagatt taatattctt tgatacacaa ggttcaaata
     4081 atggttctgt aagtcatgtt ggaatataca taggaaatgg agatatgata catgcatctt
     4141 caggttcatc aaaaaaggta acaatatcta atataaatag tagttactat tcaagtagat
     4201 atgtaaatgc aagaagagtt ttataattat tttttattac aaaacctaat aaacagattt
     4261 ttctttattg cttataatat gaaagcaggt acaatattga tagtaatgta tgttataata
     4321 aatattacta tttgatttgt acctgctttt ttatttattt tctctgttta tagaaaataa
     4381 attatattta tattttttat aaattttgac acaacagtaa attataaatt atcaaataat
     4441 agtagtttat ataaataaaa attttagaac attttttgag attcttctaa ctcgtaagcg
     4501 tccattaaag cttcgccaaa tctttgatag tgaattattt ctctttgacc taagaatttt
     4561 agtacatcta taacatcaac atcatctgtt aggtttataa gttgataata agttgcaagt
     4621 gctttttgtt cagctgccat atcctcatgt aaatcagtta cggggtttga cataactgct
     4681 ataggtctta catcaaatgg gacaccattt gaatctgttg gataaattcc atatccattt
     4741 tgtgcatagt ttgaaccaag acctgcagct tttaactctt ctgggcttgc atcagaagtc
     4801 aactgataaa ccatagtaca tataagctca acgtgagcta gctcttctgt accaatatct
     4861 gttaaaagtg cacgcatatt accagtaggc attgtgtatc tttggcttaa atatcttaaa
     4921 gatgcagcaa gttctccatt tggtccacca aattgagtta taagaaattt agccattcta
     4981 gggtcacaag tttttatatt aactgggtgt tgtatagttt tctgatatat ccacatgaat
     5041 tttcaatcct ccaatacttg atgtctaata attaaattct ctgtcccaag gccaaggttg
     5101 gtcagcccat tgccaagggc atctacttgg agcataccca aagttagtta aaggaccata
     5161 tttattttcg taagcatatc tagcttgcgc aaattgatta cataatgaat tataagtgtt
     5221 gatagcattt ttatcatcag ggtgattgtc taaatataag ttcatatcaa cacatgcaaa
     5281 agatgtttcc tgaatttttc tcataagttc agctctagat gagtctttca ttatcttcta
     5341 cctcctctat atttttttgg cttagcatat aattctttgt tgtacatttc agattctagt
     5401 aagtttagtt ctggaaatat agttccaaat tttaatgcat cagaaaggca gtacatttct
     5461 gtaaaaattt gattattaat gtatggtcta gccaaagatg gaccacaatt ttttttgata
     5521 tctctagtct tattttccaa gatgaaaacc tccttgttgt aaatagatag gtaactctat
     5581 ctagtgtata atattcaatc gtttaaaaat cgttactaat aataaaatat taaatgagaa
     5641 aaataaaaaa gataataaaa gaaagagatt atttatgtta ttataagttt atgtgttatt
     5701 gtaaactata aattatttag taaaattaaa gtaaaaatta tggtattttg tggatgaagg
     5761 tagacttttt tgtaaaatat tattaacata aatatatgag gtgattaaat gagtgagcta
     5821 tcaggaaaag gctgtgaaat tatagttcct tttgaggaaa gacttccagt aagagatata
     5881 gaaaaaagta taataaaaaa atatagaaaa aatttatggt ccaagtttat gaaggcaata
     5941 agagattata aattggttga agaaggggat aagatagctg tagcaatttc tggtggaaaa
     6001 gacagtattc ttatggctaa aatgtttcaa gagttaaaaa aacatggaca agttaatttt
     6061 gatgttgaat ttattgctat ggatcctgga taccatgcaa atataagaca attacttata
     6121 gataattgtg agtatctaaa tataccaata catttatttg attcaagaat atttgagata
     6181 gcagatgaaa tagcaaaaga ttatccatgc tatatgtgtg caagaatgag gagaggagca
     6241 ttatattcaa aagcagaaga gttagggtgc aataaacttg cattaggtca tcattatgat
     6301 gatgtaatag agacaacaat gcttaattta ttgtgtgctg gaaactttaa aacaatgctt
     6361 cctaaactca attctactaa ttttgaaggt ataaaaatta taagaccact ttattatata
     6421 agagaagaac atattataag atttattcaa aatagtggga tatggccatt aaattgtgca
     6481 tgtatggttg ctgctaaaaa aacaggcaat aagagatatg agataaaaga tttaataaag
     6541 agtttagaat caaattttaa aaatgtagaa aagtctatat ttaaggcagc agaaaatgta
     6601 aatcttgact cagtacttgg ttggcaaaaa gatggggaga aacattcttt cttagaaaat
     6661 tttgaataaa aataattaaa aagaatcact ataaacaatt agaaagggtg tttatattga
     6721 aaagagtata tatagatttt gaaatgaata tgccaaattc taaatcaaaa agagcagtat
     6781 ttaattctga tataattgca atcggagcag ttatgtatga tgaaaaaaca aaaaatattg
     6841 ataaatttaa atctttaata agaccagtaa gtaatgagga attgtatcct catatacaag
     6901 aattaacaca tatatcttct gaagaattga agagtgcacc aagttatgaa gaggtcatga
     6961 gaaagtttaa aaaatggctg ggcatatttt ctgatattaa aggaatatat acttttggta
     7021 atttagattt gacatgtttc aataacacag atatgagaag tgctaaaaaa aataatcatc
     7081 caaggtttgt aaacaatatt agaaatctat ttgtagatat aaaagaaaaa tatataaatt
     7141 gtggtatgag atgtatgaac tatatatcat tgaaaaattt acttgaattt gttaatttag
     7201 agtttagtgg agatgctcat gacccactaa atgatgcata taatttattt attttggacg
     7261 aggcaattac aaataatata gacatacaaa atcttttgat aatacgtgat ataataagag
     7321 caccatttaa tgacataaat tctaatttag ataattgttt tgataaattt aaggaatcac
     7381 tatataaaaa agaagaaaat tacaatattg tagatttttc agttgaaata ataaaaactg
     7441 tgagaatgta cttattaaca ataatagatg taaatataca aaatttagaa gttatgaaag
     7501 atataagtaa aaaaatggat actatagata aattgaaaga tatagaggaa ggctattttt
     7561 atttattaga agatgtatat tttgatatga aagatatatt agaagattta atgctttata
     7621 ggatgcatga agatgagtat aagcatgaga tagaaaatat aataaaaatg cttgatgaag
     7681 acttagataa tgaaaaaata tatataaata aatgtaatga cttaaatgtt gttaataaga
     7741 tataagaaat tataaataat agagtagaac ttgataatta taacaatttt ttataaaact
     7801 gaaataaaat taaattaata taaaaataat atacaataaa aaatgtagag agatgattac
     7861 tttctacatt tttattgtat attactttag gtttaaaaat gtattatatg ctttataatt
     7921 aatatatttt atagatattt tatctttata aaatataaaa actacttatt gcgaaagtaa
     7981 ttgatttgtt gtattataat ttacatgaat tatgtaagac aatcaaaatt cagtattata
     8041 agagtacata tattcaaatt tttaacaatt aatttatata attaaaaaat gaagggtagg
     8101 atgaagtcat gggttttaga gaagtaaaaa ttgaggaact acagtttaat ccatttacta
     8161 agattggcaa agaatggtta cttataacag ctggtgattc agaaaagttt aacacaatga
     8221 ctgctagctg gggtggtgtt ggagtatatt ggggtaaaaa tgttgttaca acttacataa
     8281 gaccacagag atatacaaaa gaatttgtag atagtaatga tacatttaca gttgccttct
     8341 ttgatgaaac gtatagagaa gctttaaata tttgtggtac tatttctggt agagatataa
     8401 ataaaataga aaaagcagga cttacaccat attttgtgga tgacacagta gcttttgaag
     8461 aagctaatat gattatagta tgtaaaaagt tatatcatgg taatatgcca ccagaaaact
     8521 ttgatgctaa agaaaatgat aaaaaatggt atccagagaa agactatcat actatgtaca
     8581 tttcagaaat tataaaagtt cttgtaaaag aataattata ataaaaagta tctaaaaaga
     8641 acttattata agtaaatgat tgtactttct tatcttatat ttaagtagat gctaataagt
     8701 aaataaatat taattttaaa taaatactat tataaaaaag atgaactata tttgctagag
     8761 ttcatctttt tatttatgta agcacttata taagtagaga tttacttgag taaaagtatt
     8821 tatttgagat atttgagtac actttataag tatttttatg ctaactagaa atacttaatt
     8881 tattgtattt gtatttgatt tattttaatg taaataattt caaggataat tttattaaat
     8941 atattactat aaaagtataa ataacattta ataaaaatat atattatatt aggtgtttta
     9001 aataaaattt ttaaagggtg attttttatg gaaaatataa ctagttattt tacaacaata
     9061 ctatgtgtat ttatatgttt atcatctgta tttatattta ctcaactagc tagagtattt
     9121 attaataaga agaagattaa tcaaaaaatt aaatcaagaa aaggatttag gtatgataga
     9181 gattttattg aagctagaag agaagaaata catataaagg ataacaataa taataaaaat
     9241 aagagtaaca ataagaaatt aaagggagaa aaagtattta aatatgataa tggagattta
     9301 tataaaggtg agtttgttga tgggaagaaa aatggatttg gaatatatat tttttcaagt
     9361 aaggaaaagt atgaagggtt atggaaagat gacaagatgc atggaatagg aaaatatact
     9421 tataaagatg gttcaatata tacaggtgaa tttaaatacg gattaaaaaa tggtttgggg
     9481 aaattaacat atcccaataa tgatatttat aagggatatt ttttagataa taagaaaaat
     9541 ggtaagggta tattatataa aaatgatgga aataaacaag caggaatatg ggaagatgat
     9601 gaacaatgta aaagtttaga ttttaaagat ttaagtaata atgaaataag taaggtttct
     9661 caaaacagat taaataacaa taaattaaga gtttagttat tctaataaaa aaatctttat
     9721 ttatgatgga atatagaata tgtttatgct gtaatttgta aaatcttcct atatcatata
     9781 aaattaagta ttgattagtc tattacataa atatagaata taaatatatc catttgataa
     9841 taaggagaag agatatgaaa gagtatgtag ttgtggattt agaaactaca ggattagatc
     9901 catataaagg ttgtgaaata atagaaatag gaataacaga aataaaaaat gaacaaattg
     9961 taaaaaacta ttcaaggttg ataaaaccta aggggataat ttcttcattt ataacagaat
    10021 tgacacatat aagtaatgaa atggtagaaa atgaagaacc tctagaatta gttttaccta
    10081 gatttagaaa gtacataggt gatagaacta taattgcaca caatgcgaaa tttgatttaa
    10141 aatttttaaa ttactatctt agaatgttaa atttagaacc aataaacaat tatatatgta
    10201 cagtagaatt attaaaaaaa tgtaaaagtt ataaaggaaa aaataagaaa cttgaaacag
    10261 cttgtgctta ttacaacata gagaatataa atgctcatag agctgatagt gatactttag
    10321 caactgcaaa actatttttg aaaataaaag atgaatatta atcataaata tagagaaaat
    10381 tagtgaaaat atcctttata aaattgaccc ttaaacttac aattttgttc tttgatttat
    10441 tttgtagaac aatgtataat gtaccttata tgaagaaggg agactaaaat gggttattac
    10501 aatgataatg ataataataa aataagacgt aaaaaaatta gtaacagcaa agatagtaat
    10561 aaaaaatcta aacattctta tacttcggca aataaaaaaa ataaaagttc taagacaagt
    10621 tctagtaaaa gaatgagcga aaaagtagat tcacagaatc ttataaaaag agatagagat
    10681 agttttacaa gagataatgt aagaccttta aatacagatt ataatcaaag agaaagaaga
    10741 agagtcgagt ataaacaaag agagaaaaaa ctaaaaagta aaaaaagaaa aaaacaaaaa
    10801 aggattagaa tagttagggc attgttatct attatgttag ttgcactaat tatattttca
    10861 gtggcaggtg caatatttac agtttcagct ataaaagggt caccagaagt cactaaaaag
    10921 ttaattaggt ctaaatatat aagtagtgag gttgtaagta taaaacaaat gccagatgat
    10981 ttaaaacatg caatagtttc catagaagat gagagatttt ataagcataa aggagtagat
    11041 gttatttctt tagttaggtc agtactaaac aatgtgttta cagatacaac acaaggtgga
    11101 agtactatag atatgcaagt ttcaaaaaat ctgcttacta gtgatgataa aactatgaaa
    11161 cggaaaataa gggatatgta taatgctaag caaatgaata agattatgac taaggatgaa
    11221 attcttgaag cttatttaaa taatatgtac ctaggtaaaa atgcatatgg agctgcaaag
    11281 ggagctgaag tctactttgg taaaaaagtt aatgaattaa atttagctga gtgtgctatg
    11341 ttagctggta tgacaaataa tcctgcaagg tatattgacc atggagaggc taagaagaga
    11401 caattgattg tattgtataa gatgcatcag cttggttata taactgataa ggagtataga
    11461 atagcaaaag ctgatgacac tccatttaaa tctgaaattg attaaaattg taaagatgtg
    11521 gtatttagaa cagttcaaac aaatgaaaat gtaataaaaa aggtgatttg ccacccatct
    11581 gtcaccataa ttataatctt atggcaaatt gttttaattc acaaattaga taatagaata
    11641 ttgttttaag tgctatagta tgctaaaatt taaaattgta ttttataaat taaaaaaagt
    11701 tataacttag ttttctaggg ttccgcattt tataatggtc aggtccaaga gaaaacgcac
    11761 aattattgtg tacacggaag gataaaagcc tgggagatat ttaataatat ttctataggc
    11821 ttttttattt ttatatattt taaatttaag gaggttttta atgaaaaaat ttgttattga
    11881 ggatacattt tggaatctat ttcctaatgc gaaaattgga gttattattt gtaatgatat
    11941 agataactct ataaaagatg aagactataa gaaaatcatt ttgcagggag aagaagaagc
    12001 attaaaatac ttggaggatt cagaatttag caaaaaccaa gtgattaaag tatggagaga
    12061 agcatttaaa aaatttaaaa cgaaaaaagg agcaaggtct tctattgagg cattattaaa
    12121 aagagttcat aatggaaata atataggaac tattaaccct ttagtggata tctacaattt
    12181 tatctcatta aaatatgcat taccttgtgg aggggaggat atagataaat ttataggaga
    12241 tattaggcta actagagcaa ttggtaatga ggagtttatc ccactaggga cagatgaaaa
    12301 tttatttcca tatgaaggag aaataatcta taaagatgat ggtggagcaa tttgtagatg
    12361 ttggaattgg cgtgaagcag tgagaacaat gcttactgaa gatactaata acgccttttt
    12421 atgtattgaa ttagtagatg aaagtagatt taaagaattt gaaaatgctt taagtgaatt
    12481 agctaaaata gttcaagaaa aattgggtgg gaagtgtaaa atctcaattt tagatattga
    12541 taataaagaa gtatcagtag attaatctgg aggtgctgtt gtaatgggct attctagtta
    12601 taaagatact tatatttagg gtaagaaggt ctattttaga tgcaacgatt atcaaaggat
    12661 atgatataga aaataaaaat gtggaatata aaaagccttt aaaataagaa aaaattgtat
    12721 tttaaaggct tttattatac tattttttat ttgcacaatt aacaataaaa atatattgtt
    12781 aattataata tttt
//
LOCUS       DAFNNB010000113        12590 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0114, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000113 DAFNNB010000000
VERSION     DAFNNB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12590
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0114"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..650
                     /locus_tag="L3M60_002613"
     CDS             <1..650
                     /locus_tag="L3M60_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBN6227264.1"
                     /translation="KIIKVQKPKFTGELEIIENLEDILEDIFNDEKIFNKIIENEVIS
                     DLDSVRVSFESCIFRNVSFENCNLKKIDLLDVVFENCNLSNTFFDGGSIYRVEFKNSK
                     LIGVRFDDCILKNVLFKDVLAKYSNFSFSKFKIVSILDSNFENSVFQEVKGDNLVFEY
                     TNLKKANFNGTKLSGIDFTTNNIEGIEIGIDDIRGAIFDVSQAIDLTKLMGIIIK"
     gene            791..2062
                     /gene="brnQ"
                     /locus_tag="L3M60_002614"
     CDS             791..2062
                     /gene="brnQ"
                     /locus_tag="L3M60_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBN6227265.1"
                     /translation="MRDIFIVGFALFSMFFGAGNLMFPPFLGMESGKDWLIPLIGFVV
                     ADAGIAMLVIIATARCKGSMDDVLRRAGKGLARVMSVAALACLALLVIPRTCATTYEM
                     GIMPIFGDHGPVAKAIFSIIFFGLTLAFTIRSSKVIDIIGKYLTPALLLVLFVLIVKG
                     IISPAGPMSPEHMIDKNLFGEGISQGYQTMDALGAASMATIMLMSIIAKGYTSDKDQI
                     GMTIKAGFVACVFLAVVYGGLTYLGATVSTLYDTSIPQASLLVEITSILLYGQVGKVI
                     LGVIVALACLTTSTGLTASISTYFEGISKASYKQLVIGICVASMLISNLGVDSIIAIS
                     VPILQTIYPVLLAIVVMELAGKHIKNDNAFKGAAYVTLVISLLSAINGMTGAVPFIQS
                     LPLAGLGFNWVIPAILGGIVGNFIKSNKQVA"
     gene            2264..3451
                     /locus_tag="L3M60_002615"
     CDS             2264..3451
                     /locus_tag="L3M60_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HBN6227266.1"
                     /translation="MLRKITAFMLSICLIIGLTGCSLLVGKKDKEKVIDDFKVAVVTQ
                     PLSENKVQYNMVEEMAKEYEEENKIDKDKDGQTKVKQTIKHVVLPENFTSNIDSAINK
                     IVKLADDKEVQAIVVSTDQAGLLPALQKVKEKRPEIITISAPMGDDKNQLSQFVDVNL
                     GVSAEERGKVLAERSKEMGAKAFIHYASTDDLKDVNIAKRLEMIKETCKNIGLPFVQV
                     NTPNINAEEDKNKVKQFLNEDIEKQVKKYGKDINVFGVNDYMDEVILTKALELKYIVA
                     EQSNPSPIQTYPSVMGLKISKKDAQNYDKINDMISEKAKAFGMSNRLGGYPMPMDAFL
                     PSLAIYLATEMVKQDLTQEDVCDPDYLEAFTELRFGIGSEFTPLTEVLYNYQSVILSQ
                     LIY"
     gene            complement(3681..4127)
                     /locus_tag="L3M60_002616"
                     /pseudo
     CDS             complement(3681..4127)
                     /locus_tag="L3M60_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009899983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4172..4651
                     /locus_tag="L3M60_002617"
                     /pseudo
     CDS             4172..4651
                     /locus_tag="L3M60_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890933.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4653..4949
                     /locus_tag="L3M60_002618"
     CDS             4653..4949
                     /locus_tag="L3M60_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227267.1"
                     /translation="MSEVVKKEVQVKKGNVIDAFIGGARNGFQISTNSMAPNVIFGFA
                     IISVFNLTGLLDVIGTIFTPVMSIFGLPGVVATAIMTIVISRRGFPLLYTKTKM"
     gene            5344..6534
                     /locus_tag="L3M60_002619"
     CDS             5344..6534
                     /locus_tag="L3M60_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBN6227268.1"
                     /translation="MSKYNFDRVIDRVGTDCVKWDFRTNCSTKAQEDGLPFWIADMDF
                     ECAEPIIEALRKKVEHKIFGYSSNDSDKYFNAVCDWYKRRFNWEINRKDIIFSPGVVP
                     AVAILVRILTNSNEGVIIQKPVYYPFEAKVKSNNREVVNNPLIYENGTYRMDYDDLEE
                     KAKCSNNKVLILCSPHNPVGRVWREDELKKVVEICKKYDLWIIADEIHSDLIRKGFKH
                     TPLQSLCPEYKDKIVTCIAPSKTFNLAGMQLSNIIVNNDELKKKYQEEVTAVGVATSP
                     NPFAIVATIAAYNKSEDWLNELNEYLDNNIQFIDEYLKENLPKVKLVYPEGTYLAWLD
                     FTAYGLNEVELEDLMFKKANVLFDEGYIFGKEGIGFERINVACPQSLLKECMGRLKTT
                     FENL"
     misc_binding    6668..6923
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6982..7983
                     /locus_tag="L3M60_002620"
     CDS             6982..7983
                     /locus_tag="L3M60_002620"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBN6227269.1"
                     /translation="MSTIIPKDYKSSLNVIDTQIAIKKLKDFFEMRLSNELNLIRVSS
                     PLFVLPETGANDNLNGEKAVSFDIPFMNKNAEIVQSLAKWKRLALKKYGFEVGSGLYT
                     DMNAIRKNEELDNIHSLYVDQWDWELVIDKSSRNEKTLKDVVKRLYGVFKDTEIFVCS
                     MYEDIKEILPEEITFITSQELEDMYPELSPKEREDKIVKEKKAVFITQIGKTLISGEK
                     HDGRSPDYDDWELNGDILFYNPVLDSALELSSMGIRVDEESLDRQLKLAGCEERKEFD
                     YHKMLLNGELPYTIGGGIGQSRICMYFLQKAHIGEVQVGVWPQDMIQNCSSAGIELL"
     gene            8087..8476
                     /locus_tag="L3M60_002621"
     CDS             8087..8476
                     /locus_tag="L3M60_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HBN6227270.1"
                     /translation="MDFLKIIINIVLDILKKILVRFKNAKFGLVFVFDLLKLPDFMTD
                     KRINIADKIKVISVLIFTISYFVSGVDIIPEMIAGAFGFIDDVIVLIWSIGIVNEEIN
                     KYRVIAKKDKHSTIIENVNFSIKDEEE"
     gene            complement(8641..8787)
                     /locus_tag="L3M60_002622"
     CDS             complement(8641..8787)
                     /locus_tag="L3M60_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBN6227271.1"
                     /translation="MLLYGTNKKTILDRLGHIDIKIAINKYSHVLEEVKKEISENLSK
                     ILFK"
     gene            9088..9543
                     /locus_tag="L3M60_002623"
                     /pseudo
     CDS             9088..9543
                     /locus_tag="L3M60_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894433.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
     gene            9547..10377
                     /locus_tag="L3M60_002624"
     CDS             9547..10377
                     /locus_tag="L3M60_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6227272.1"
                     /translation="MKICITVGHSILKSGACTSADGVVNEYQYNKSLAPVLADTFRKE
                     GHKVDVITCPERQFSSKNEEKSHKIPRVNSGGYDLLIELHLNASNGQGKGSEVLYYSN
                     KGLEYATRVCDKLGTVFKNRGAKLDKNLYILNSSKPTAVLIESFFCDNKEDYESAKKL
                     GYEGMAKLIVEGILNKGVANNEVKQMYKHTIVYSGDDKVSAEILGLYYKRAKENYLVS
                     DIKDYEPHQTQNLYVIGGETCNKMKEMSNTTGEKFTEIYSDDVWSTMDKAIEFVKEKL
                     "
     gene            10652..12307
                     /locus_tag="L3M60_002625"
     CDS             10652..12307
                     /locus_tag="L3M60_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227273.1"
                     /translation="MNLDFHYYGTYLAAKVAGYNDTDAKTIAYAAQYVDESDKSMILD
                     DVNFTLPTIQTNLEFKKYYADLASWGYKWDFESLNEIKKVWIPFHFLPGNLNNQYKYN
                     GVKESKCLTTSWKFKDGDDQKFRLMCLPNSETVSAIINDLIYFHSTEEYKLQFIGMRM
                     HVLADTWAHMYFIGKPEWYINDVKEFIAEESKYIEETKWTKAEEYRNNGWHSINFTGT
                     PDIGGYESISYLGHGRMGHIPDYGYLNYRYIPNWSSTDDKVKIEKNNQDNFFKAFCQM
                     VYALKCIKNGNDFNINQYDNLTAQQKTEVSKVIATRKNDQSEAWKKAIQALGYNPLEK
                     FDKNKWKNEFIKSSNKEKTDYYYFNLVAKKHVDYVTDFLEEKRLSLEEMFNGYCKFKN
                     FDDTQFAKNRNRVTKIILRGAYIVDAIQLVYDGKYKTPMHGEPFGGTMVSLDLDTDDY
                     IVKISGSIGLYEGGEPYPNSPTRTIGKITFLTKKGKTITAGHETMFKLYRNFTLEAPA
                     GKQIFALKGSYLIRKLREETSKRYLDCLEIANTKDCSVATSTK"
BASE COUNT         4837 a         1324 c         2203 g         4226 t
ORIGIN      
        1 ctaaaattat taaagtacaa aaacctaaat ttacaggaga attagaaatt attgaaaatc
       61 ttgaagatat acttgaagat atatttaatg atgaaaaaat atttaataaa attatagaaa
      121 atgaagttat aagtgattta gattctgtaa gagttagctt tgaatcttgt atatttagaa
      181 atgtatcctt tgaaaattgc aatttaaaaa agattgattt acttgatgtt gtatttgaaa
      241 actgcaattt atcaaataca ttttttgatg gaggaagtat atatagagtt gaatttaaaa
      301 atagtaaatt gataggtgtt agatttgatg attgtatact taaaaacgtt ttgttcaagg
      361 atgttttagc taagtattct aacttttcgt tctctaaatt taagatagtt tctattttag
      421 attcaaattt tgaaaattct gtatttcaag aagtaaaagg tgacaatcta gtatttgagt
      481 atacaaactt aaaaaaagca aactttaatg gaactaaact ttctggcata gattttacta
      541 ctaataatat tgagggaatt gagattggta tagatgatat tagaggagca atttttgatg
      601 tgtcacaagc aattgattta acgaaattga tgggaattat cataaaatga aaaaaatatt
      661 cataagataa attgggaaaa cgttaacaat gtgtgaattt ttaccagatt gacatttttc
      721 tgaaaataaa gtaagattat cttaatattc aaaatatttt caaagggggt aatttagtgt
      781 gagtaagcag atgagagata tttttatagt tggttttgct ttattttcta tgttctttgg
      841 tgcaggaaat cttatgtttc ctccattttt agggatggaa tccggcaaag actggttaat
      901 tcctcttatt ggatttgttg ttgcagatgc tggtatagca atgcttgtta ttatagcaac
      961 agcaagatgt aagggaagca tggatgatgt acttaggaga gctggtaaag ggttagcaag
     1021 agtaatgagt gtagctgctc tagcatgttt agcacttctt gtaataccaa gaacttgcgc
     1081 aacaacatat gaaatgggta ttatgcctat atttggagac catggaccag tagctaaagc
     1141 tatattttca ataattttct ttgggttaac tttagcattt actattaggt cttctaaggt
     1201 tatagacata ataggtaagt atttaactcc agcgttatta ttagtattat ttgttttaat
     1261 agtaaagggt ataatttctc cagcaggacc tatgagtcct gaacatatga tagataaaaa
     1321 tctatttggt gaaggtatat cacaaggtta tcaaacaatg gacgctctag gtgcagcatc
     1381 tatggcaact ataatgttga tgtctataat agctaaagga tatacatcag ataaagacca
     1441 aataggaatg actattaaag ctggttttgt agcctgtgta tttctagcag tagtatatgg
     1501 tggtcttact tatttaggtg caacagtttc aactttatat gacactagta tacctcaagc
     1561 atctttatta gttgaaataa ctagtatatt gttatatggt caagtaggaa aggtaatttt
     1621 aggtgtaatt gtggcacttg cctgtctgac tacttctaca ggtttaacag catcaatatc
     1681 aacttatttt gaaggaataa gtaaggcgag ttataaacaa ctggttatag gtatatgtgt
     1741 agctagtatg cttatatcaa atttaggtgt tgattctata atagccatat cagttccaat
     1801 cctacaaact atatatcctg ttttattagc aatagttgtt atggagttgg ctggtaaaca
     1861 tataaagaat gataatgctt ttaaaggtgc agcatatgtt acattagtga ttagtttatt
     1921 atcagctata aatggaatga caggagctgt tccatttata caaagcttac cacttgcagg
     1981 tttgggcttt aactgggtaa taccagctat attgggtgga atagttggta actttataaa
     2041 aagcaacaaa caagtagcat aataatttgt aattaattta attttataat atattaattt
     2101 ttagtactat gggtaggttt aatttaaacc taatctaccc atagtacttt ttagattgga
     2161 ttagaatagc atgtatataa ttttttataa tataattttt ctttgaagta tgataaaatt
     2221 agtaatagtg ataagaaagt acaaatttag gaggttttaa ttaatgctta gaaaaataac
     2281 agcatttatg ctatctatat gtttaattat tggacttaca ggttgtagtt tacttgtggg
     2341 caagaaagat aaagagaaag ttatagatga ttttaaagtt gcagtggtaa ctcaaccatt
     2401 atctgaaaat aaagtacaat acaatatggt ggaagagatg gcaaaagaat atgaggaaga
     2461 gaataagatt gataaagaca aagatggtca aactaaagtt aaacaaacta taaagcatgt
     2521 tgtacttcca gaaaatttta cttctaatat agatagtgct attaataaga tagtaaagtt
     2581 ggctgacgat aaagaagttc aggctattgt agtttcaact gaccaagcgg ggcttttacc
     2641 tgccttacaa aaggtaaagg aaaagcgtcc agaaataata actatttctg cccctatggg
     2701 agatgataaa aatcaattaa gccaatttgt tgatgtgaat ctaggggtta gtgcagagga
     2761 aagaggaaaa gtattagctg aacgttcaaa agaaatggga gcaaaagctt ttattcatta
     2821 tgcctctaca gatgatttga aagatgttaa tatagccaaa agattagaaa tgattaaaga
     2881 gacttgcaaa aatataggat tgccatttgt acaagttaat actccaaata taaatgctga
     2941 agaagacaaa aacaaagtta agcagttttt aaatgaagat attgaaaaac aagttaaaaa
     3001 atatggaaaa gatataaatg tatttggtgt aaatgattat atggatgaag ttatattgac
     3061 taaggcttta gaattaaagt atatagttgc agagcaaagt aatccatcac caatacaaac
     3121 atatcctagt gttatgggtc tgaaaatatc taaaaaagat gctcaaaatt atgataaaat
     3181 aaacgacatg ataagtgaaa aggctaaggc ttttggtatg tcaaatagac ttggtggata
     3241 tcctatgcca atggatgctt ttctaccatc attagcaatt tatcttgcca ctgaaatggt
     3301 aaaacaagat ttaacacaag aagatgtatg tgacccagac tatttagagg cgtttacaga
     3361 attaagattt gggataggtt cagaatttac acctctaaca gaggtacttt ataattatca
     3421 atcagtaatt ttaagccaat taatttatta atattaagga agtgtctcaa aatgaacttt
     3481 ttagtttatg aggcactctt tttgtattaa ataaaatata tttttatctt tttaacaatt
     3541 aaaaagaagc ttatctctat gttgagatag cctcgttttt aacataaata agttttatta
     3601 ttttataaaa aatttataga aaaataaaaa attataatta tatataaatt ttgataaagt
     3661 aaagatgaat tactataggt ttaaattata ttatttttta ttatttttat gcctttctca
     3721 gttattgatg agccttttct tcctttacta gaaattaagt atccattgcg tgtcaaatat
     3781 tctagcatat ttcttacttc gtattctgtg acattatatt ccataaattt cattaacttt
     3841 gtcctcccca ttggtttcat gtgcaatgat tttaggatag aaatgcatat gtttttgtca
     3901 tattggctta aatcattgaa tatggacata ttacttgaca tatgattatt atttaatttt
     3961 gaagatatgt tttgaggtaa atcatttatt gtaagataat tagagcccat gtaagccata
     4021 tattcaacac aatttctcag ttctctaata tttcccatcc aagtgtatga atttaaagct
     4081 tctaaaagac tcttatcgag ttttatgttt ttatagggaa gttctttcta agtaaagact
     4141 tatttacaaa agtaactttg ttaaaagtat tatagctgca aactttagag acgatggagg
     4201 tactggttct agagaaggat ggttatatgc attgacacta ataccatcag ttatgtttgc
     4261 acttggagta gttagagtta tagaccactt agatggaatg aaggcagctc aaaagcttct
     4321 tagtccacta ttaaagccat tattagggtt acctggattt gcagggctaa ccttgatagc
     4381 tagtttacag tctacagatg cagctgcatc tatgactaaa gaacttaaag atgatggata
     4441 tatagatgaa aaacaaaaag ctgtattctg tgcatttcaa ttttcaggcg cctctgcaat
     4501 tacaaatttc tttgcttctg gagcagcact atttccattt ataggggatg taccaatatt
     4561 tataccactt actttaatat taattatgaa gtttgttggg gcaaacttac ttagattata
     4621 tttaaataaa tttgacaagg gggaagctta acatgagtga ggtagtaaaa aaagaagtac
     4681 aagttaaaaa aggaaatgtg atagatgcgt ttattggagg agctagaaat ggattccaaa
     4741 taagtaccaa ttcaatggct ccaaatgtca tcttcggttt tgctataatt tctgtattca
     4801 atttaacagg tcttttagat gtaataggga ctatatttac acctgtaatg agtatatttg
     4861 gacttccagg tgttgtagca acagctataa tgacaatagt tatatcgcgg agaggattcc
     4921 cattattata cacaaaaact aaaatgtaaa actacatata ttggattagg agctgatgct
     4981 acaccagggt ttcataatcc aaatgtaaca tttgatacga aagctttaga atatggtgta
     5041 gattggttga aaagagattg ggataatatc caaattaata attctacatt taaaattata
     5101 attatagaag ttatagaaaa agattgctta tgttaattga aaaataatta atatgggcaa
     5161 tcttattttg ctataatgaa acttattttg aaatattgaa aaaatatggt agaaaaagca
     5221 attatatttt aagtctattg ggcaaaaaag tgatattata atattaagga ttataaaatg
     5281 atatcaaatt gaatctagta atacactaac aattataaaa attttatttt taggagggaa
     5341 aaaatgagta agtataactt tgacagagta atagatagag taggaacaga ttgtgtaaag
     5401 tgggatttta gaactaattg ctcaactaaa gcacaagaag atggtttgcc tttttggatt
     5461 gcagatatgg attttgaatg tgcagaacca ataatagaag cattacgtaa aaaagttgaa
     5521 cataaaatat ttggatatag ttccaatgat agtgataaat actttaatgc agtttgtgat
     5581 tggtataaaa ggagatttaa ctgggaaata aatagaaaag atattatttt tagccctggt
     5641 gttgtacctg ctgttgctat tttagtaaga atattaacta attctaatga aggcgtgata
     5701 attcaaaagc cagtgtatta cccatttgaa gctaaggtaa agagtaataa tagggaagtt
     5761 gtaaacaatc ctctaatata tgaaaatggg acttatagaa tggattatga tgatttggaa
     5821 gaaaaagcta agtgtagcaa caataaagta ctgatacttt gtagccctca caatcctgtt
     5881 ggaagagttt ggagagaaga tgaattaaaa aaggttgtag aaatatgtaa aaaatatgac
     5941 ctatggataa tagctgatga gatacattct gatttaatta gaaaaggatt taaacatact
     6001 cctttacagt cattatgccc agagtacaag gataaaatag ttacatgtat agctccaagt
     6061 aaaacattta atctagctgg aatgcagttg tctaatataa tagtgaataa tgatgaacta
     6121 aaaaagaaat atcaagaaga ggttacagct gttggagttg caacttcacc aaatcctttt
     6181 gctattgtag ctacaatagc agcatataat aaaagtgaag actggcttaa tgaattaaat
     6241 gaatacttag ataataatat acaatttatt gatgaatact taaaagaaaa cctaccaaaa
     6301 gtaaaacttg tttatccaga aggaacatat ctagcatggt tagattttac tgcttatgga
     6361 ttgaatgaag tagaacttga ggatttaatg tttaaaaaag ctaatgtatt atttgatgaa
     6421 ggttatatat ttggaaaaga aggaatagga tttgaaagaa taaatgtagc ttgtccacag
     6481 tctttattaa aagaatgtat gggcagatta aagactacat ttgaaaattt ataaagtata
     6541 ggtaaaaagt aataaagtgt ttaaaaataa aaaaataata agcttgacaa attttaacta
     6601 atcacttatc atactattat gaataaatag aaaacataag agagctaaat tttattgaat
     6661 taaactctta aagactttga aaggaagagt agttatagaa tgtaattaaa gcgagttggt
     6721 gatggtgtga gaccaatatg aagtctatag tgaagaacat cctggagttg ctaaccgaaa
     6781 ttttaaaaga gtaggcttag tcggaaccaa accgttatca tattggggtg tatcgagtaa
     6841 tatctgtaca ttgtgagctg ggtttgaatt tatttcaaac taatcagggt ggtaccgcgg
     6901 aaaagcaacc tttcgtccct tttatatagg gatgaagggt ttttttaatg gaaaaatata
     6961 taaatatagg gagtgaatga tatgagcaca ataataccaa aggattataa aagtagtttg
     7021 aacgtaattg atacacaaat agctataaaa aaattaaaag atttttttga aatgagacta
     7081 agtaatgaat taaatcttat aagagtttca tctccgttat ttgtattgcc agagacgggt
     7141 gcaaatgata atttaaatgg agaaaaggct gtaagctttg atataccatt tatgaataaa
     7201 aatgcagaaa ttgttcaatc acttgcaaaa tggaaacgat tagctcttaa aaaatatggg
     7261 tttgaagttg gcagtggttt atatacagat atgaacgcaa taagaaaaaa tgaagaatta
     7321 gacaatatac attctcttta tgtagaccaa tgggattggg aattggttat agataaaagt
     7381 agtagaaatg aaaagactct aaaagatgtt gtgaaaagat tgtatggagt atttaaggat
     7441 actgaaattt ttgtatgtag tatgtatgaa gatataaaag aaatattacc agaagaaata
     7501 acttttataa catcacaaga gttagaagat atgtatccgg aattatcacc aaaggaaagg
     7561 gaagataaaa tcgtaaaaga gaagaaagca gtgtttataa ctcaaatagg aaaaacatta
     7621 atatcaggag aaaaacatga tggaagaagc cctgattatg atgattggga attaaatgga
     7681 gatatattat tttacaatcc tgttttagat tcagcactag aattatcttc aatgggaatt
     7741 agagttgatg aagaatctct tgatagacaa ttgaaacttg ctggatgtga agagagaaaa
     7801 gagtttgact atcataagat gcttttaaat ggagagcttc catatacaat tggtggagga
     7861 attggtcagt caagaatatg tatgtatttc ttacaaaaag ctcatatagg agaagtacaa
     7921 gttggagtat ggccacagga tatgattcaa aattgtagca gtgcaggaat tgaattatta
     7981 taatttaaat ataagctaat taataaaatt tatgtctaat atgttcatca aattaattat
     8041 atcggttata atgtagaata acctataatt ataagaggtg ataaatatgg attttctaaa
     8101 aataattatt aatatagttt tagatatttt aaaaaaaatt ttggtgagat ttaaaaatgc
     8161 taaatttggt ttggtttttg tatttgattt acttaaacta cctgatttta tgacagataa
     8221 aagaatcaat atagctgata aaataaaagt aatttctgta ttaattttta ctatatctta
     8281 ctttgtatct ggggttgata taattccaga gatgattgct ggggcatttg gttttataga
     8341 tgatgtgata gttttaattt ggagtatagg tatagtgaat gaagaaatca ataagtatag
     8401 agttattgca aaaaaagata aacattcaac tataattgaa aatgtaaatt tttctataaa
     8461 agatgaagaa gaataataat ttatatgtgt atgctaacat attatataac atcatatatt
     8521 actagatagt agctaaatcc tagtttttgc tactgtctag tgataaaaat ttgactgacc
     8581 tccaactgag aaacaaaaaa tatacgatta actaacatcc aacgaacata aaactgacta
     8641 ttatttaaat aagattttgc tcaaattttc agatatttct tttttcactt cttctaaaac
     8701 atgagagtat ttatttatag ctatttttat atctatatga cctaatctgt ctaaaatagt
     8761 ttttttatta gttccatata ataacatcaa agttacattt gtatgtctaa gctcatgtaa
     8821 ttttatatat ttaaagatgt ttttaaaaga tttagactaa tatcataagt attcttaata
     8881 cattgaataa ctatgatttt attatatcaa atctaagtta taaatactat ttgatgatag
     8941 agtagttgta tacagaaata tatagatgaa cttttgaaaa agtaataaag tagaggatta
     9001 tataattaaa cataatggat aagtagaagg agcagaacca atagtataga aggttctttt
     9061 tttatgcgtt tagatgattt ttaagtcttg ttttatgtta aaataatagg taatacagat
     9121 ataacaatag tttttttagc aataaatata tttataaaat tggcaatatt aagaatagca
     9181 ttttatgctt tgctagaatg cttaagagca ataaaaacac ataaacttaa tggttctctt
     9241 gtaatcaatg gaggaaataa gaaaagtcgc aatgctggtt tgtatatttt ttctagaatt
     9301 agttgacata cttataaatt ttaacttttt atttatgttg ctataagatt gggttgattt
     9361 cttatgatta aataatctag gaatatcaga atttttcttt attttattta ttttgtataa
     9421 aaatctaagt atattaaaaa atatgtactt aaatggatta ccagttatta aagattaaaa
     9481 gagaaaatag gtagtttatt agataccatg gcagatgaat caactattaa aggaggagaa
     9541 taaataatga aaatatgtat aacagtagga cacagtattt taaaaagtgg agcatgtact
     9601 tctgctgatg gagtagttaa cgagtaccaa tataacaaat ctcttgcacc agtattagca
     9661 gatacattta gaaaagaagg tcataaggta gatgtgataa catgtcctga aagacaattt
     9721 agttctaaaa atgaggaaaa atctcataaa atacctagag ttaatagtgg aggatatgac
     9781 ttacttatag agctacattt aaatgcaagc aatgggcaag gtaaaggttc agaagtacta
     9841 tattatagta ataaaggttt agagtatgcg actagagtat gtgataaact aggtacagta
     9901 tttaaaaata gaggtgctaa actagataaa aatttatata tcttaaatag ctctaaacct
     9961 acagcagtat taattgaaag tttcttctgt gataataaag aagattacga gagtgctaag
    10021 aaacttggat atgaaggaat ggctaagtta attgtagaag gtatattaaa taaaggtgtt
    10081 gcaaataatg aggttaagca aatgtataaa catacaatag tgtacagtgg tgatgataaa
    10141 gtgtcagcag agattttagg attatattat aagagggcaa aagaaaatta tttagtttca
    10201 gatataaaag actatgagcc acatcaaacg caaaatcttt atgttatagg tggtgagact
    10261 tgtaataaaa tgaaagaaat gagcaatact acaggagaaa aatttacaga gatttattct
    10321 gatgatgtat ggtcaacaat ggataaggct atagagtttg taaaagaaaa attataattt
    10381 aaaattttta tttaaacaag aatgtagata agaaaaattt atatcaaaat ttttcttatc
    10441 tattttttct tataaatata tttattaaag ttataaaagc atatatttct agaaaaaatg
    10501 tgttttttga ctatttatgc gtttttgatg accagttaga caataaggat tgaatatttt
    10561 gaatatttgt tttattctta aaaaaaaata aatatatata aataattata tatatttatt
    10621 ttttttaaaa ataaaattaa gaggtgattt tatgaattta gattttcact attatggcac
    10681 atatcttgca gctaaagtag ctggttataa cgacactgat gcaaagacaa ttgcttatgc
    10741 tgctcagtat gtagatgaat cagataaaag tatgattctt gatgatgtaa actttacact
    10801 tccgacaata cagactaatt tagaatttaa aaaatattat gcagatttgg cttcatgggg
    10861 ttataaatgg gatttcgaga gtttaaatga aataaagaaa gtatggatac catttcattt
    10921 tttacctggg aatttaaaca accaatataa atataatgga gttaaagaga gtaaatgttt
    10981 aacgacaagt tggaaattta aagatggaga tgaccaaaaa tttaggttga tgtgtttacc
    11041 aaatagcgaa actgtttctg ccattataaa tgaccttatt tattttcata gtaccgaaga
    11101 atataagtta caatttattg gaatgagaat gcatgtactt gcagacacgt gggcacatat
    11161 gtattttatt ggaaaaccag aatggtatat taatgatgtt aaagaattta tagcagaaga
    11221 aagtaagtac atagaagaaa ctaaatggac taaagcagaa gaatatcgta ataacggttg
    11281 gcattctatt aactttactg gtactcctga tattggtgga tatgagagca tatcatatct
    11341 tggacatggt agaatggggc atataccaga ctatggatat ctaaattata gatatattcc
    11401 aaactggagt agtactgatg ataaagtgaa gatagaaaaa aataaccaag ataatttctt
    11461 taaggcattt tgccaaatgg tttatgcatt aaaatgtatt aaaaatggaa atgactttaa
    11521 tataaatcaa tatgataacc taacagctca acaaaaaaca gaagtttcaa aagtaatagc
    11581 aacaagaaaa aatgaccaat cagaagcatg gaaaaaagca atacaggcat taggatataa
    11641 ccctttagaa aaatttgata aaaataagtg gaaaaatgaa tttataaaat cttctaataa
    11701 agagaaaaca gattactatt attttaatct agtagctaag aaacatgtag attatgtaac
    11761 tgatttttta gaggaaaaaa gattgtcttt agaagaaatg ttcaatggat attgtaaatt
    11821 taaaaatttt gatgataccc aatttgctaa aaatagaaat agagtgacaa agattatact
    11881 tagaggagct tatatagttg atgctataca acttgtatat gatggaaaat ataaaactcc
    11941 aatgcatggt gaaccatttg gaggaacaat ggtatctctt gatttagata ctgacgatta
    12001 catagttaag ataagtgggt ctataggttt atatgaaggt ggagaaccat atcctaattc
    12061 tccaactaga acaataggaa aaataacttt tttaactaaa aaaggaaaaa caataacagc
    12121 aggtcatgaa actatgttta aattatatag aaatttcact ttagaagcac ctgcaggaaa
    12181 acaaattttt gcacttaaag gttcatattt aatacgtaaa ttaagagaag agactagcaa
    12241 acgatatttg gattgtttag aaattgcaaa tacaaaagat tgttcggtag ctacaagtac
    12301 gaaataacta catattggat agctagtgtt ataatatatt taagaaaata gtataatatt
    12361 tatatttaca atgaaagcac tctttattat aaagagtgct ttcattgtaa atcatcaaga
    12421 acattactaa atatacctta aaattgattt aaataggtct ttttatttag aaataacagt
    12481 catataagtt aaatattaat atctaaacaa tttaataatg aatatagaaa gattaaaagg
    12541 tagcaactag aactagttat taccttactt ttttctgatt ttttatatct
//
LOCUS       DAFNNB010000114        12588 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0115, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000114 DAFNNB010000000
VERSION     DAFNNB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12588
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0115"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(57..1451)
                     /locus_tag="L3M60_002626"
     CDS             complement(57..1451)
                     /locus_tag="L3M60_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227274.1"
                     /translation="MTKLNKKLSISISIVVVVVYIVSITINSMFIHRYYLHEKRNILD
                     NIENEIKDKDIDKLKSDIDSIEEKNNADIVYIELDKNHKDKGNIDRINQDLLNAFWKK
                     GLSLNKFWIEENSLKNIGKKSINKIYNQGKTKYSLLVKFIKKDNYLFAISIPIEHSEE
                     TIGIVNRFNIIMGVFSVVIITILTFILSNRVIKPIEKLKFLSKDISELNFRIEDIKTN
                     DEIEELAYSINMMSEKLEKAHNELNKRNENLKTFISDASHEIKTPIALIKAYAIGMKD
                     GLDDGTYTDTIIEQAENMTNTINSLLYWAKYEKKERSLCLVDLKERLYKSLKNYELLI
                     DKGGISVKCNVDDRELIISSDEDSIEVVFNNLISNAIKYTNNNEIEINLFEENERIIL
                     SIKNGIDCDMEDEIENIWKPFYVLEKSRSKELSGTGLGLTIVRTILEENNFRYRVEIC
                     DKIIAFYIIFIKLI"
     gene            complement(1444..2091)
                     /locus_tag="L3M60_002627"
     CDS             complement(1444..2091)
                     /locus_tag="L3M60_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227275.1"
                     /translation="MNILIADDEISMLKILCAYFKKENFNVLTAKNGEEALDIFYKNK
                     IDLAILDWMMPIIDGIEVCKEIKENSNTKVLMLTAKSQSEDEIEALNIGADEYIKKPF
                     DPRVLIIRAKKLVNYKDTINIKDLKIDFESSKVFKGDNELLLTKKELELIRCLINNKG
                     MILSREKLLDLVWGLDYEGDFRTVDTHIRRLRVKIGEDIIKTYRGLGYSLEDFND"
     gene            complement(2526..3365)
                     /locus_tag="L3M60_002628"
     CDS             complement(2526..3365)
                     /locus_tag="L3M60_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBN6227276.1"
                     /translation="MSDIKNEVAKKTLSKKDVIKSWLRWFFFAQSNYNYERLQSTAFS
                     HSMLPVLKKLYPDKEELKQEVETHLAFFNTEPICGCVIHGITIAMEEEKANGADEISG
                     DGMNAIKTGLMGPLAGIGDTLTQGVITPIVLAVCIGLTEGGASIAGPILFVIAQYIIM
                     TSISFGMWTNGYKYGKKAVESILQGGIVNKVIEGASILGTLVMGGLVGRFINLSTPIS
                     YTSGDFKFSLQTDLLDKIFPGLIPLVLTLLVLFALKKGLSPIKIMFILIVVGAISGIV
                     GLF"
     gene            complement(3389..4147)
                     /locus_tag="L3M60_002629"
     CDS             complement(3389..4147)
                     /locus_tag="L3M60_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6227277.1"
                     /translation="MHISLIQAVLIAIFYYLSWSPWLTYVGFFTWNRPLLAGFVTGII
                     LGDPVQGAIIGAGINMIYLGFISAGGAQMGDPAFAGYVGTALAIASKLDVSTAMAIAV
                     PLGTVATVLWIGKMTVNSFFAHWADREVQKGNVDRVAFINIVPPQILLFAMSFIPALL
                     VVYFGPNAIDGMLEVMNENVLHVFNVIGAMLPALGIAMNLKLIGNKFTMPFFILGILM
                     AVYFKVDIIVISVIGVILALTITSIKYGKDSATS"
     gene            complement(4204..4677)
                     /locus_tag="L3M60_002630"
     CDS             complement(4204..4677)
                     /locus_tag="L3M60_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBN6227278.1"
                     /translation="MSNIALTRIDDRLIHGQVITAWCKITSAKRIVIVDDLVVKDPFI
                     VQVLQMAAPSTVKVEVHDVESGAEVLKSHNGDENLIVLVKYPKTVLGLVNNGVDLKEL
                     NVGGMGAGLGRKSFYKNISVSDEEKEIFRELIAKDVKCFIQIVPDAKKIDVGTLL"
     gene            complement(4706..5122)
                     /locus_tag="L3M60_002631"
     CDS             complement(4706..5122)
                     /locus_tag="L3M60_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6227279.1"
                     /translation="MIHMAGIIIATHGNMAKAMLESAELIVGKQENVETLGLNHGDSI
                     DEFSRKLEDGIEKYKDEGVLVLLDFYGGTPFNTSAVIINKFAKVCELECLTGVNLPML
                     LELFLNRDTMKLKDLKNLCEEVGISGIKDVKTVLNL"
     gene            complement(5168..6220)
                     /locus_tag="L3M60_002632"
     CDS             complement(5168..6220)
                     /locus_tag="L3M60_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBN6227280.1"
                     /translation="MKSVRFYGIRDTRVEDVDVPKILEKDDVIIKVKVAGICGSDISK
                     YSKTGPHMVGEILGHEFSGEVAQVGTEVKSFKIGDRVAVCPAMPCFECDECKKGLYSR
                     CNNVAIIGNKELGGCFAEYTKVKERNLIKIPDEISYETAAALEPVCIAGHGLFRSEAK
                     VGDTVVVLGTGPIGLFSIQWAKIFGSTKIIAVDVFDEKLDLAKELGADICINAKEKNI
                     VEEIKRLTDRDGADIVIESAGTPMTCGQVLLLAKKGGTILYAGVPYGDVVLTREQFEK
                     IVRSELTVKGTWFGNSFPFPGKEWSTGLYHMKKGAMNVEKLVTHRINLEEVPAYFEKV
                     YKRDIFFGKIMINIGN"
     gene            6616..7296
                     /locus_tag="L3M60_002633"
     CDS             6616..7296
                     /locus_tag="L3M60_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBN6227281.1"
                     /translation="MKKVYQGKTKDVFELDNGNYLLKFKDDVTGKDGVFDPGENSVGL
                     SIDGIGRANLETSIKFFEILNNAGIKTHYVSANLEEATMEVLPAKVFGNGLEVICRYR
                     AVGSFLRRYGSCVEEGAKLDCYVEATLKDDDRCDPLITSEGLEALNIMTQAQFDSMKS
                     MTQKISNIVRDTIAEKGLELYDIKFEFGFYNDEVILIDEIASGNMRVYKDGVIVDPMD
                     LTALLLDK"
     gene            complement(7426..7746)
                     /gene="sugE"
                     /locus_tag="L3M60_002634"
     CDS             complement(7426..7746)
                     /gene="sugE"
                     /locus_tag="L3M60_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBN6227282.1"
                     /translation="MKWLFLFVAGLFEVFWAFQLKNSHGFSKLTPSILTVVGMIVSFY
                     FLSLALKHLPLGTSYAIWTGIGTVGTAIVGMAILNEPISISRIACIGFIVVGIVGLKL
                     LSTN"
     gene            8128..8955
                     /locus_tag="L3M60_002635"
     CDS             8128..8955
                     /locus_tag="L3M60_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBN6227283.1"
                     /translation="MNFYKIAVCQMITTENKIENINHAVDMVTEAAINGAKIVVLPEM
                     FNCPYENKYFPKFAEEYPGETTTILSKLAEKHGIYLVSGSIPELEDGKIYNTCYVFDK
                     NGDLIGKHRKMHLFDIEVTGKVSFKESDTLTAGNDVTVIDTEYGKVGVAICYDIRFPE
                     LSRLMALKGAEIVILPASFNMTTGPAHWELSIRMRALDNQIFYVGAAPARNMNASYIA
                     FGNSRISDPWGKIIAQADEKECIIYADIDRDLIPDIRQQLPLLKHRRTDLYELNTLK"
     gene            9748..10422
                     /locus_tag="L3M60_002636"
     CDS             9748..10422
                     /locus_tag="L3M60_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227284.1"
                     /translation="MNNKILVVDDEEGIITLLKDYFEMNSYEVYTAQSGKDALKKISK
                     NPDIILLDINIPEIDGIEVCNRIRDYVSCPILFLTARIENTDKINGFRAGAGDYIVKP
                     FDLDELGARVSAHLRREQRKSNQTKIKFYNDLTIDYEKRLICIDNNQISLSKKEFDIV
                     ELLSMNPGQVFDKEKIYDRVWGYNSNGNSDVIMEHIRKIRIKLSKHTLENYIETIWGV
                     GYKWIK"
     gene            10410..11570
                     /locus_tag="L3M60_002637"
     CDS             10410..11570
                     /locus_tag="L3M60_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6227285.1"
                     /translation="MDKINNMSLKKVFFVLTLSSLIIASILTTFSYILLNNLYNYIQD
                     KYLKTPINTGFITIVQNNVNQFSDYDKTLLNIINILQLVLPLIFFIGLLLLADIIFYK
                     VKLKTPIEILNKGAMEISNNNLDFCLEYNNNDELGNLCNAFEKMRSELNKNNIKMWTM
                     INDRKQLNAAFSHDLRNPLTVLKGYSNYLTKYIPTGKLSDEKILSTTQLMSEHINRIE
                     YYVENMSNAQRLEDLVVSKSMSNINKFIKNLDENISIIAKQEGKSFTLKSQINNVNLL
                     FDENIILRVVENIISNAFRYARNNVSILIYIEQELLTFVIEDDGIGFSKESLKSALRP
                     FYRDKTLNESNAHFGMGLYISKILCEKHGGSISIENNLTNSAKITAKFSTKN"
     gene            11744..12412
                     /locus_tag="L3M60_002638"
     CDS             11744..12412
                     /locus_tag="L3M60_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227286.1"
                     /translation="MIIKAKQLSKIYGSNNNKVIALNNVNLEINSGEFVSIIGPSGSG
                     KSTLLHILSGLDNPTSGQVLLDDKDIYKHSEKELSALRRKSFGFVFQQFNLLPVLTAS
                     ENISMPVLLDKKQPDKDYLNEISSLLGIADRLNHLPHELSGGQQQRVAIARALIAKPD
                     IIFADEPTGNLDSKSGSEVMNLLIKTSKQFGKTLVVITHDDRIAKLADRKISIIDGVL
                     MEVK"
     gene            12415..>12588
                     /locus_tag="L3M60_002639"
     CDS             12415..>12588
                     /locus_tag="L3M60_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227287.1"
                     /translation="MKNYLSLSIKELKSQKLMTTFIIIAIVLSTIMTTVVGQSIGILQ
                     NLRIEQARSFNGDR"
BASE COUNT         4410 a         2111 c         1430 g         4637 t
ORIGIN      
        1 tgaaaaacaa ataattgcta tattttttta attattcttt atatttgcat tttaattcaa
       61 attaatttta taaatataat ataaaatgct ataattttat cacaaatttc aaccctgtat
      121 ctaaaattat tttcttcaag tatagttcta actatagtaa gaccaagacc agttccagaa
      181 agctccttac ttcttgactt ctctaaaaca taaaaaggct tccatatatt ctctatttca
      241 tcttccatat cgcaatctat accatttttt atggataata taattctctc attctcttca
      301 aaaagattaa tctctatttc attattatta gtatacttta tagcattact aattaaatta
      361 ttaaatacca cctctatact atcttcatcc gaacttatta taagttctct atcatctaca
      421 ttgcacttaa cacttatccc acctttatct atcaacagct catagttttt taagctttta
      481 tataatcttt cctttaaatc taccagacac agacttctct ccttcttctc atattttgcc
      541 caatataaaa gagaatttat tgtattggtc atattctctg cttgttcaat aattgtatct
      601 gtataagtcc catcatccaa gccatctttc atacctattg catatgcttt tataagtgct
      661 attggagttt ttatttcatg agatgcatct gatataaaag tttttagatt ctcatttctt
      721 ttattcaatt cattatgtgc tttttcaagc ttttcgctca tcatgtttat gctgtaagct
      781 aactcctcta tttcatcatt cgtttttata tcttcaatcc taaaatttaa ttcagatata
      841 tctttagata aaaactttaa cttttctata ggttttatga ctctgtttga taatataaac
      901 gtaagtatag ttattataac tactgaaaaa actcccatta ttatattaaa tctatttaca
      961 atgccaatag tttcttcaga atgttctatg ggtatagata tagcaaacaa ataattgtcc
     1021 tttttaataa attttacaag taaactatat tttgttttac cttgattgta tatcttattt
     1081 atacttttct tcccaatatt ttttaaacta ttttcttcaa tccaaaactt atttaaactt
     1141 aaccccttct tccaaaatgc atttaataaa tcttgattta ttctatctat attaccttta
     1201 tctttatgat ttttatctaa ttctatatat acaatatctg cattattttt ttcttcaatt
     1261 gaatcaatgt ctgattttaa tttatctata tctttatctt taatttcatt ttcaatatta
     1321 tccaatatat ttcttttctc atgtaaataa tatctatgaa taaacatact atttatagtt
     1381 atagacacta tataaacaac tacgactact atcgatatac ttatggacaa ttttttattc
     1441 agtttagtca ttaaaatcct ccagactata ccctaatcct ctataagttt ttattatatc
     1501 ctctcctatt tttactctta atcttcttat atgagtatct actgttctaa aatcaccctc
     1561 ataatctaaa ccccatacta aatctaaaag tttttctcta gataaaatca tccccttatt
     1621 atttattaaa caacgaatta attctaattc tttcttagtc aataatagtt cattatcacc
     1681 tttaaaaact ttacttgact caaaatctat ttttaaatct ttaatattaa ttgtatcttt
     1741 atagtttact agtttcttag ctcttattat taaaacccta gggtcaaatg gttttttaat
     1801 atattcatct gcaccaatat tgagtgcttc tatttcatct tcactttgac ttttggcagt
     1861 caacataagt acctttgtat tactattttc ctttatctct ttacaaacct ctattccatc
     1921 aattattggc atcatccaat ctaatattgc taagtctatc ttatttttat aaaaaatatc
     1981 taaagcctct tccccatttt ttgcagtaag tacattgaaa ttttcctttt taaaatatgc
     2041 acaaagtatc ttaagcatac taatttcatc atcagcaatt aatatgttca tttattcaca
     2101 tccctaaaat tcattattat atttaatatt atatccatta ctttaattat ataaaatata
     2161 tgttacagga agtttacaac tatttataat acattagtta tatattataa tacctcgaca
     2221 atatcttgat tatatatttt aatatgtaac taatatatta taatatttta attgtataaa
     2281 tttatatttt tgtgaaatta cttaatattt tatatgaaaa tgaaaaagca cctcattctg
     2341 attaaaaatc tttctgagat actttttaaa atattaaata ctaaatatag cttatataag
     2401 cttaaatttt aatctattat aaaattaccc taattgctaa actttttacc ataagttttc
     2461 ctacatctta caaatataat caatagatgt agaatcataa tttacacacc aacatatata
     2521 tttatttaaa ataatcccac aatacctgaa atagctccca ctactattaa tataaacatt
     2581 atttttattg gagaaagtcc tttctttaag gcaaaaagta ctaataatgt aagtaccaaa
     2641 ggaattaatc ctggaaatat cttatcaagt aaatccgttt gaaggctaaa tttaaaatct
     2701 ccactagtat aacttattgg tgtagacaaa ttaataaacc tacctacaag tccccccatg
     2761 actaaagtac ctagtataga agcaccttct ataaccttat ttactattcc accttggagt
     2821 atactttcaa cagctttttt cccatactta tatccattcg tccacattcc aaaagaaatt
     2881 gaagtcatta ttatatattg agctattaca aataatatag gccctgcaat tgatgctcct
     2941 ccctcagtta aaccaataca aacagctaat actataggag ttataactcc ttgagttaaa
     3001 gtatcaccaa tccctgctag tggacccatc aagcctgttt tgatagcatt cataccatct
     3061 ccacttattt catctgctcc attagctttt tcttcctcca tagctattgt tattccatgt
     3121 ataacacatc cacatatagg ctctgtatta aaaaatgcta gatgagtctc cacttcttgt
     3181 tttagctctt ccttatctgg atataacttt ttaagtaccg gtaacattga atgtgaaaaa
     3241 gctgttgatt gcaatctttc ataattgtaa ttagattgtg caaagaaaaa ccatcttaac
     3301 cacgatttta ttacatcttt cttgcttaac gttttcttag ctacttcatt ttttatatct
     3361 gacataatat tttccccctc acatgtattt aagatgtagc actatctttt ccgtacttaa
     3421 ttgaagtaat tgtaagagca agtattactc caataacaga tattactatt atatctactt
     3481 taaaataaac agccattagt attcctaata tgaaaaaggg catagtaaat ttgtttccaa
     3541 tcaattttaa attcatagca attccaagtg ctggcaacat agccccaatt acattaaata
     3601 catgtaacac attctcattc attacttcca acataccatc tatcgcattt ggaccaaaat
     3661 aaacaactaa tagtgctggt ataaagctca ttgcaaataa cagtatctgt ggtggtacta
     3721 tatttataaa tgctactcta tcaacattcc ctttttgaac ctctctatca gcccaatgtg
     3781 caaaaaaaga atttactgtc atctttccaa tccaaagtac agttgcaaca gttcccaatg
     3841 gtacagctat tgccattgca gtacttacat ctagcttaga tgctatagct aaagcagtgc
     3901 ctacatatcc tgcaaaagct gggtctccca tctgagctcc tcctgctgat ataaaaccta
     3961 gatatatcat atttatacct gctcctataa ttgctccttg aacagggtct cctaatataa
     4021 ttcccgttac aaatccagcc aatagaggtc tattccatgt aaaaaatcca acataagtca
     4081 accatggact ccaagaaaga taataaaaaa ttgctatcaa tactgcttga attagtgaaa
     4141 tatgcatact tttcctcctt attttcttct aaagatttac aagataatct tctttataaa
     4201 gctttataat aatgttccta catcaatttt ctttgcatct ggtactattt gtataaagca
     4261 tttaacatcc tttgctatta gctctcgaaa tatttctttt tcttcatctg acacagatat
     4321 atttttataa aaactttttc taccaagtcc agctcccata cctccaacat tcaattcctt
     4381 taaatctacc ccattattta ctaaacctaa aacagtttta ggatatttga ccaatactat
     4441 taaattttca tctccattat gtgattttaa aacttcagca cctgattcta catcatgtac
     4501 ttcaacctta acagttgatg gagctgccat ttgtaaaact tgaactataa atgggtcctt
     4561 tactactaaa tcgtccacga ttactatcct ttttgctgat gtaatcttac accaagcagt
     4621 aataacttgc ccatgtatta atctatcatc aattcttgtt aatgctatat tactcataat
     4681 aattcctcca gatttttaat ttattctata aatttaaaac agttttaaca tctttgattc
     4741 ctgatatccc aacttcctca cataaattct tcaagtcttt caacttcata gtatctctgt
     4801 ttaaaaataa ttctaaaagc ataggtaaat ttacacctgt caaacattct aactcacata
     4861 ccttagcaaa tttatttatt ataacagcag atgtattaaa tggagtccca ccataaaaat
     4921 caagcaatac taaaactcct tcatccttat atttctcaat tccatcttct aactttctac
     4981 taaactcatc tatgctatca ccatggttta atcctaaagt ctctacattc tcctgtttac
     5041 ctactattag ttcagcactt tctaacatcg ctttagccat atttccatgt gtagctataa
     5101 ttattcctgc catatgaatc atacctttct taactaaact tttttatatg gattattctt
     5161 tagtactcta attacctata ttaatcatta tcttaccaaa gaaaatatcc cttttatata
     5221 ctttttcaaa atacgctggt acttcttcta aatttattct atgtgttact aatttttcaa
     5281 cattcatagc accctttttc atatgataca gtccagtact ccattctttt cctggaaatg
     5341 gaaaggagtt tccaaaccaa gttcccttta cagttaattc acttcttact atcttttcaa
     5401 attgttctct agtcaaaact acatccccat acggtacacc tgcatacaat attgtccctc
     5461 cctttttagc taacaacaac acttgaccac acgtcattgg agtaccagcc gattcaataa
     5521 caatatctgc accatctcta tcagttaacc tttttatttc ttctacaata ttcttttctt
     5581 ttgcatttat acaaatatct gcccctaact cttttgccaa gtctaacttc tcatcaaata
     5641 catccacagc tattattttt gtcgagccaa atatctttgc ccattgtatt gaaaataatc
     5701 ctattggacc tgttccaagt actacaacag tatctccaac tttagcttct gacctaaaaa
     5761 gaccatgtcc tgctatacat actggctcta gtgcagcagc agtttcataa cttatctcat
     5821 ctggtatttt tattaaattt ctctctttta cctttgtata ctctgcaaaa caacctccaa
     5881 gttctttatt tcctattatt gctacgttat tacatcttga atacaatcct tttttacatt
     5941 catcacattc aaaacatggc atggctggac aaacagcaac tctatctcct attttaaagc
     6001 ttttcacctc tgtaccaacc tgtgctactt ctccagaaaa ttcatgccct aaaatctctc
     6061 caaccatatg tggacctgtc tttgaatatt ttgatatatc agagccacat attccagcta
     6121 cttttacttt aattattaca tcatcttttt ctaatatttt tggtacatct acatcctcca
     6181 ctcttgtgtc tcttatacca tagaatctaa ctgacttcac gattaaccac ccctactttt
     6241 tatttgagaa tatatactta ttttttatat gttaaatatc taaagcaact ttcatgccaa
     6301 tatatgtaag tattgttttc atgcacttta taaaattatc attagatgtg tgtcaaagtg
     6361 tgtcaaaact tttttatacg aaaacttatg tgtgtcaata taaacgacac tcacatttta
     6421 tactagttct cctattataa gttttataaa atgtactttt tttgttattt ttataaaatt
     6481 gttcggaaat tccccctcta tttgtgatat aatattaaat ataaggtatc aatataaatt
     6541 ttcatacgaa tatcagaact tttttgacac taatgcctta ttcaatataa taatttaact
     6601 ttttggaggt tgtttatgaa aaaagtgtat caaggaaaga ctaaagatgt ttttgaatta
     6661 gataatggaa attacttact taaatttaaa gacgatgtaa ctggtaaaga cggagtattt
     6721 gacccaggcg aaaatagtgt aggtctaagt atagatggaa ttggtagagc taatttagag
     6781 acatctatta agttctttga aattctaaac aatgctggaa ttaaaactca ttatgtaagt
     6841 gctaatttag aagaggcaac tatggaagtt ctaccagcaa aagtttttgg taatggatta
     6901 gaagttattt gtcgttatag agctgttggt agtttcttac gtcgttatgg ttcatgtgtt
     6961 gaagaaggag ctaaactaga ttgttacgta gaagcaactt taaaagatga tgaccgttgt
     7021 gaccctctta taacatctga aggtttagaa gctttaaaca ttatgactca agctcaattt
     7081 gattcaatga aaagcatgac tcaaaaaatt tctaacatag ttcgtgatac tatagctgaa
     7141 aaaggtcttg aactttatga tatcaaattt gaatttggat tctacaatga tgaagtaatc
     7201 ttaatagatg aaatagcatc tggaaatatg cgtgtttata aagatggtgt aatagttgac
     7261 cctatggatt taactgctct tttattagat aaatagtata tacatataaa tttcaaatat
     7321 aataaactta taaataagat aagctatgaa gtaaagactt tttaaatgtt gattattttt
     7381 aaacattttc tcgcctttaa tttcatagct tatttaaatt atactctaat tggtagacaa
     7441 taactttaac cctacaattc ccaccacaat aaaacctata catgcaattc ttgatatact
     7501 aataggctca tttaagatag ccatgcctac aatagctgta ccaacagtac caattccagt
     7561 ccatattgca tatgaagttc caagtggaag atgttttagt gctaatgaca aaaaataaaa
     7621 acttacaatc attccaacaa cagtaagtat acttggagtt aatttagaaa atccatgtga
     7681 attttttagt tgaaaagccc aaaacacttc aaacaaacct gcaacaaata aaaataacca
     7741 tttcataaac tcatccctct tttcaaatat atctcttatt tttccaataa aataacctga
     7801 gaaatattat aaaatatctc ccaggttttt gtccttccgt gttcacaaca aaaactatgt
     7861 tgtgggtttt atctcggacc agaccaatca ttaataattg cggaacccta taaaactcta
     7921 ttaattattc tattttatct ataataatac tatcaacata tataaaaaaa gtcaatatat
     7981 gggttccatc tataataaaa ataactttta tatacctttt aatattctta ttcacttctc
     8041 tccataccca tttaggtcaa tctaagaata tctaattttt atttaatatg ttataataac
     8101 ataaaaatat tgaaaggagt aatttctatg aatttctata aaattgcagt atgccagatg
     8161 attactacag aaaataaaat tgaaaatatc aatcatgctg tagatatggt aacagaagct
     8221 gccatcaatg gagctaaaat tgtagttttg ccagaaatgt ttaactgccc atatgaaaat
     8281 aaatattttc ctaaatttgc tgaagaatat ccaggtgaaa caacaacgat attgagtaaa
     8341 ttagctgaga aacatggaat ttatttagtt tctggtagta ttcctgagtt agaagatgga
     8401 aaaatctata atacttgcta tgtatttgat aaaaatggag atctaattgg aaaacatcgt
     8461 aaaatgcact tatttgacat tgaagttaca ggaaaagtta gttttaaaga atcagatact
     8521 cttacagctg gaaatgatgt cactgtaata gatactgaat atggaaaagt tggtgtagct
     8581 atttgttatg atattcgttt ccctgagtta tcacgtttaa tggctcttaa aggtgctgaa
     8641 atcgttatct tacctgcttc atttaatatg actactggtc ctgcccattg ggagttatct
     8701 ataagaatgc gtgctttaga taatcagata ttctatgttg gtgctgcacc tgctagaaat
     8761 atgaatgcct cctacatagc ttttggcaat tcaagaattt cagacccttg gggaaaaatc
     8821 attgctcaag ctgatgaaaa agaatgtatt atttatgctg acattgatag agatttaatt
     8881 cctgacataa gacaacagtt accacttctt aaacacagaa gaactgattt atatgaactt
     8941 aatactttga aataaaacag aacataaccc cataaatcat aaattaagtg ttaaaaaaat
     9001 ttatggggtt ctcattaatt tgatttaaaa ttaatttcat aaaaataata ctaatattaa
     9061 tttaattaaa taatactaat aatactaatg attatgctaa ttatcagtat ttactaattt
     9121 aatcactatt agttatttct tttattaaat atatttactt tttttactta ttatgtcttc
     9181 tagctttaca tttggattat ggcatctctt tctactttta gtcacatctt tctattgaat
     9241 tacttgctta aaacaccaat acatacatgc cagacaatgt tcacaattat gtaaaaatct
     9301 tgctctacct tattcaatgt tgatattatc tctcctaaaa tttcttttaa ttaaaaaaca
     9361 acattcgttg atatatcgag tatgttttaa gcaactgaaa aaaacaataa taattgatat
     9421 atcaactaat taatgtagat taattcattt ttataattta tatacttttt aaccttttta
     9481 attaaatcct tcctttctat ttatccattt taaacttaaa ttataagatt tcatcagctg
     9541 tgtttttgca ttccaataag tattaaatat caaatatata ttctattaaa atttctaata
     9601 atttcatcct tgatttattt aacatataaa aatatactaa ctttaatgat taagttttga
     9661 aatataacta agaatttgag aatttattga gatttttata ctaaagtata catactatac
     9721 aaacttaatt tagaagggag cattcttatg aacaataaaa tactcgtcgt agatgatgag
     9781 gaaggtataa taacactctt aaaagattac tttgaaatga atagttatga ggtttacact
     9841 gctcaaagtg gaaaagacgc attaaaaaaa atatccaaaa atccagatat tattctactt
     9901 gatataaaca tccctgaaat agatggaatt gaagtttgta atagaattag agattatgta
     9961 tcatgcccta ttctatttct tacagctaga attgaaaata ctgacaagat aaatggtttt
    10021 cgtgctggtg ctggtgatta tattgtaaaa ccttttgatt tagacgaact tggagctaga
    10081 gtatctgccc atttaagacg tgaacaaagg aagtcaaatc aaactaaaat taaattttat
    10141 aatgatttaa cgatagatta cgaaaaaagg ttaatttgta tagataacaa tcagatttca
    10201 ctttcaaaaa aggaatttga tatagtagaa cttctctcta tgaatcctgg acaagtattt
    10261 gataaagaaa aaatctatga tagagtttgg ggatacaata gcaatggaaa tagtgatgtt
    10321 ataatggaac acattagaaa gatacgaatc aaactatcaa aacatactct tgaaaattat
    10381 atagaaacaa tctggggagt tggttataaa tggataaaat aaataatatg tcattaaaaa
    10441 aagttttctt tgttcttact ttatcttcac taattatagc atcaatactt actactttct
    10501 catacatttt gcttaataat ctatataatt atatacaaga taagtattta aaaacaccta
    10561 taaatacggg ttttataacc atagttcaaa acaatgtgaa ccaatttagt gattatgata
    10621 aaacgttact taatataatc aacatacttc agcttgtact tccacttata ttttttatag
    10681 gacttttatt attagctgat ataatttttt acaaggtaaa actaaaaaca cccatagaaa
    10741 tactcaataa aggtgcaatg gaaatatcca acaacaattt agacttttgt ttagaatata
    10801 ataataatga tgaattaggt aatctatgta atgcttttga aaaaatgagg tctgaactaa
    10861 ataaaaacaa cataaagatg tggactatga ttaatgatag aaaacaactt aatgctgctt
    10921 tttctcatga tttaagaaat cccctaacag ttctaaaagg ttactcaaac tatttaacaa
    10981 aatacatacc aacaggcaag cttagtgatg agaagatttt atcaaccaca cagcttatgt
    11041 ctgagcatat taacagaatt gaatactatg tagaaaatat gagtaatgcc caacgattag
    11101 aagacttagt tgtttcaaaa tctatgtcaa atataaacaa gtttataaaa aacttagatg
    11161 aaaatatatc tatcattgct aaacaagaag gaaaatcttt tacacttaaa agtcagataa
    11221 ataatgtaaa tctattgttt gatgaaaata tcattcttag agtcgtcgaa aatataatat
    11281 cgaatgcttt tagatatgct agaaataatg tatccatact tatatatatt gaacaagaac
    11341 ttcttacctt tgtaattgaa gatgatggta taggatttag caaagaatct ctaaaatcag
    11401 ctttaagacc tttttataga gataagactt taaatgaaag caatgcacac tttggtatgg
    11461 gtctttatat ttcaaaaata ttatgtgaaa aacatggtgg ctctatttct attgaaaata
    11521 atttgactaa cagtgctaaa attactgcta aattctcaac aaaaaattaa agcaatatta
    11581 aatcaatttt ttaggctttc actcctcttt tataaacttt ataacttaga gtttacaaag
    11641 tgtttttttg aaataaattt ttagtaaaca aaattaaaaa gttgagaaaa aattgagttt
    11701 taaaacttac actcttctta tatagaaaag gagagtgttt tttatgatta taaaagcaaa
    11761 acaattatca aaaatatatg gttctaataa caataaagtt atcgcactta ataatgtaaa
    11821 tttagaaata aactcaggtg aatttgtttc tataattgga ccttctggaa gtggaaaaag
    11881 cacactttta catattttaa gtggattaga taatcctacc tctggacaag tgttacttga
    11941 tgataaagat atatataaac acagtgaaaa agaactatct gcacttagaa gaaaatcctt
    12001 tggattcgtt tttcaacagt ttaatctttt acctgttctt acagcatctg aaaatatttc
    12061 aatgcctgta cttctagata aaaaacaacc tgataaagac tatttaaatg aaatttcatc
    12121 attacttgga attgcagata gactcaatca tcttccccat gaactttcag gtggtcagca
    12181 acaaagagtt gcaattgcta gagcacttat tgcaaaacca gatataatat ttgctgatga
    12241 gcctacagga aacttggata gtaaaagtgg cagtgaagtt atgaatcttc ttataaaaac
    12301 ttcaaaacag tttggaaaga ctttagtggt aattactcat gatgatagaa ttgcaaaact
    12361 tgctgataga aaaatttcaa ttattgatgg tgttcttatg gaggtgaaat agaaatgaag
    12421 aattaccttt cactttcaat aaaagaatta aaaagccaga aacttatgac tacattcatc
    12481 attatagcaa tagtactatc aactattatg acaactgtgg ttggacagtc aattggtatc
    12541 ctacaaaatt taagaattga acaagcccgt tcttttaatg gtgataga
//
LOCUS       DAFNNB010000115        12347 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0116, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000115 DAFNNB010000000
VERSION     DAFNNB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0116"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            263..1597
                     /locus_tag="L3M60_002640"
     CDS             263..1597
                     /locus_tag="L3M60_002640"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HBN6227288.1"
                     /translation="MRVIIIGGVAAGMSAAAKLKRIKPEYEVVVYEKTEIVSFGACGL
                     PYFVGGFFDDADELLARTPEKLREAGIDLNIFREVIEVDSESKKIKVKNVKTGEIYED
                     YYDKLMIATGARSIMPPIKNINLKNISTLKSLYDGEYLKKLLSNDENRRVTIIGAGFI
                     GLEAVEACKKLGKDVDVIQLEDRILPQVFDKEITDVLEEEINRHNVNLHLDEVVVELY
                     GEDKVEKVITNKGEIDTDVVIIATGVRPNTEFLSSTNIKMLKNGAIIVDEYGRTSVED
                     IYSAGDCATIKNIVSNENVYVPLATGANKLGRIVGENLAGREVSYQGSLSSSCIKIMD
                     MEAASTGITERKAKDLGINVKSKFISDYNQTHYYPGRNKIYVKLIYDADTKVVLGGQV
                     AGFKDAVQRANVLAAAIFGKMTTSQLGMLDLCYAPPFARTWDVLNVAGNVSK"
     gene            complement(1726..2643)
                     /locus_tag="L3M60_002641"
     CDS             complement(1726..2643)
                     /locus_tag="L3M60_002641"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HBN6227289.1"
                     /translation="MIDLTLLGCGGNVPMPNRFLSSVFINYKGRKILVDCGEGTQVSM
                     KLKKCGFKDIDLICITHLHGDHIFGLLGLLSTIGNSGRTSDLTIVGPIGIVDCIRSMR
                     NLVEYIPYTLKIIENPQGSFSLDNKVLRNLEISTISLEHSIECLGYSFNFRRNPKFDI
                     DKATKNEVPKILWNKLQEGQNIVLDSKKYTPDMVLGELRKGVKISLTTDTRPIESIPD
                     FIKNSDLFICEAMYGDDLDISKAVRNKHMTFREAANLAKLGNVKQLLLTHFSPSLDTP
                     SIYLENATNVFENTILGEDRLSLHLNFDE"
     gene            2851..3735
                     /locus_tag="L3M60_002642"
     CDS             2851..3735
                     /locus_tag="L3M60_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4846 domain-containing protein"
                     /protein_id="HBN6227290.1"
                     /translation="MKVLKKITILSLIILTLVGCSNGEDKKVVEGSKKIQNSKKNEQP
                     IIVHKDANILKDRYEPPKGFKRVNVEKGSFESFLRNTKLKNYGEKVKYYDGRTKESKG
                     IYDSVFDVDIGNRDLHQCADAIMLMRAEYLYQNKLYDEISFDFVDGFKAKYSKWVQGY
                     RISVNDSGSSWYKATEYSTSYESFRKFMDIVFAYSGTLSLEKELEPVKVEDMKIGDVF
                     IVGGSPGHASIIMDMAENEKTGEKVFMIAQSYMPAQQTQLLINRKNPNLSPWYSLDFE
                     GDLTIPQWTFKRDQLKRF"
     gene            3948..5789
                     /locus_tag="L3M60_002643"
     CDS             3948..5789
                     /locus_tag="L3M60_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HBN6227291.1"
                     /translation="MKIRRYLILVIIILITTSQSFISYGLDRFEEITIFHTNDIHGRY
                     AEGDEHIQIGNLATLKKETPNSILVDAGDCLHGLPIVNMGKGKNAVELIKCAGYDYIT
                     PGNHDFNYGKDRLFELSKSANLGENTLKFLSSNIFENGRKVFDSNDIKEINGVKIGFF
                     GLSTQETKSKTGFKNVEGLEFKNPIESATEQVSELKEKGADVIVAICHIGTNTLSKPN
                     SIDIANKVQGIDLIIDGHSHTKFENGKQVGDTLIVSTGQYLENIGEVKLTLDTDDVGN
                     IKIENKSARLIGKEEALKYKLDSSVSKKVDEIKEQQEKILDKVIGVTTNTLDGSYENV
                     RTKETNLGNLISDILLDKTKADISLFNGGNIRDTIEKGNITIRDIVDVFPFSNTIVTK
                     ELTGAQIKDVLEHGVKIYPEKSSAFLQVGGISYYFNPKQKEGERITSIQKEGKPLDLD
                     KKYVVATNDYIASGGDEFPCFSKEPILKEFGNLESAVTEYIEYKREISKNVDGRIGIK
                     SKDELISENQESDLILSENKESINDNEENLSNNIKSSDSDKNKILDKTRTRESSNLGI
                     SKREAREKSPKTGDLGFSNSIIIFIVSSTLICLLNFNQKELKNKKSK"
     gene            6095..6802
                     /locus_tag="L3M60_002644"
     CDS             6095..6802
                     /locus_tag="L3M60_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227292.1"
                     /translation="MNNILLLEDDKSLNRGISFKLKKEGYNVFSAFSIEEAKSIFAKE
                     EICLIISDIGLPDGSGFDFCEEVRKKSDVYIIMLTALDEEVDIVTGYDLGADDYITKP
                     FSLMVLISKVNALMKRVNTVKNYTLLVCDDLFFYYIENKLIVRADNKEEEIILSKTET
                     KLLKYLMENSMQTLTKEQLLESLWDSSGNFVDDNTIAVNIRRLRQKVEKNPSAPKYIK
                     TVRGVGYIWGERSIKKC"
     gene            6796..8106
                     /locus_tag="L3M60_002645"
     CDS             6796..8106
                     /locus_tag="L3M60_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227293.1"
                     /translation="MLKNMNLKDGLSVKDDYIMRRVIVYSILSILIVTIIFLLFEYSR
                     LYKLYEHQIKNTQNIAGILIEKYPNDEVDIVKSIYNSNYSKVPIGEKAFKKFGYGLEN
                     KMSNDKNFSLYLKSFFKESFFIFLMMFIVVIGIIYYFIRYINKNLSKIYFIVDKMAQE
                     NYLKDTTVSEETGYLVEYNEYFKEGTFSKINNCLYELNRSLKIKFIKLEKEKESVKSL
                     VTDISHQLKTPLASLKLYNTLLIEEELEEDEANEFLLTNKASINKLENLINSLVNISR
                     LEISMINIKKEDNDIKNTILDAVKSVTPKAKNKSITLNLNEFDSRIVPHDKKWTEESI
                     FNVLDNAVKYTQINGEINISVEETANYFKIIIEDNGIGIEKNEFNNIFKRFYRGSADE
                     IESVEGSGVGLYLSRKILEEQGGNIIVSSKVGIGSKFSLFLTTM"
     gene            8223..8906
                     /locus_tag="L3M60_002646"
     CDS             8223..8906
                     /locus_tag="L3M60_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227294.1"
                     /translation="MKILYTENLSKHYGKGESLVKALDNVNLEINEGEFVAIIGKSGS
                     GKSTLLHMIGGLDIPTSGKVYIDNKNIFTLKEEELAVFRRRKIGFIFQSYNLIPSLNV
                     WENVVLPIGLDGREVDETFIKELLKSLGLENKRDVLPNTLSGGQQQRVAIARALATRP
                     AIILADEPTGNLDSKTSDEVMSILKSMSKKYSQTLVMITHDDSIAQMADRVIFIEDGK
                     VLKVGDKND"
     gene            8899..11286
                     /locus_tag="L3M60_002647"
     CDS             8899..11286
                     /locus_tag="L3M60_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HBN6227295.1"
                     /translation="MINTTKIAKNNLKQNKSKSILIIITIILSTLMLSSIGIYIVNAG
                     AYQKENTIKYSGNYQGILANVDEKQADILSNHADVELTGEMNGVGVEKLEDDSNISLA
                     YMNEDALKLNSFEFVKGKLPTKENEIVLDSGALKALGYKNKDLGERIKISYNDYKNDK
                     KIEKEFIISGILKTSEISEAGKYYYAIISESYMRNTRNMSQEDFNIYVKFNDKSNLSI
                     EQIKEKLDKIANDIGLDTINTAVNENYINALKPDMETIMGGVFVGLVIVLSSILVIYN
                     IFYISIVTKVQEFGKLRAIGATKKQIKNIVFKEGFILAGISIPIGIILGYVLANIIIK
                     SFMNIDAKSSQLPVILLVAVISFISVVLSLLKPMKVASKVSIVDAVRYSGNKISNKNK
                     RKGYKNINLNRLSHANLERNKKRTYMTLASLILSGTIFITVSTALESFDAEKMAREHF
                     PYDIEVRLSGYEMNSDKNPKNNLNILQMDNPLSKDFFNQIKNIEGIKRIESARSVKIG
                     MEDYDVEFKYDLLQSINENDVKSLSKNLIDGKINLERLQTGDEIVITHVDTAKEMGVK
                     AGDKIRLTLYDGDKKIKKEFKVQAIAMGVPSFGIGKDFIDRTLKYDSTSSLGIYTKEG
                     KYQEVKDSIKKIAKSNGFLETDFIDSRIESNKATISFIKIMGYTLTGIIGVIGFMNLV
                     NTMITSIVTRKKELGMLQAIGLTNKQLVKMLNSEAISYTSGMMIGSILFGGILGYIAV
                     MVLKKTGLSYATYSLPIVPILLMIVCILIAQFITTYLIGRSFNKESLIDRVRYSE"
     gene            11574..12326
                     /locus_tag="L3M60_002648"
                     /pseudo
     CDS             11574..12326
                     /locus_tag="L3M60_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
BASE COUNT         5051 a         1190 c         2187 g         3919 t
ORIGIN      
        1 tatcaataat ttgcttaaat agagtaaaaa aataattttg aaaaatgtga ttaaatatag
       61 aaaaataggg tataataact atttaaggaa tttgaattgt gctaacaaaa taaaaaatag
      121 ctttgataag atttgatttc aaataaaaat gtgttaaata aataactaaa aattattata
      181 aaaaatgttt acatagaata tatataaact aaaaagctta aaataagtga agaaattaac
      241 tagacatcaa aggagagata aaatgagagt aataataatt ggtggtgttg cagctggtat
      301 gagtgctgca gcaaaattaa aaagaataaa acctgagtat gaagttgttg tttatgaaaa
      361 aaccgaaata gtatcttttg gtgcttgtgg gttaccatac tttgttggtg gattttttga
      421 tgatgctgat gaattattag caagaacacc tgagaaactt agagaagctg gaattgattt
      481 aaatatattt agagaagtta ttgaagtcga tagtgaatct aagaaaataa aagtaaaaaa
      541 cgtaaaaaca ggtgaaatat atgaagatta ctatgataaa ttgatgatag caactggagc
      601 aagaagtatt atgcctccta taaaaaacat aaatttaaag aatatttcta cattaaaaag
      661 tttatatgat ggagaatatc taaaaaaact tttaagtaat gatgaaaata gaagagtaac
      721 aataatagga gctggtttta taggattaga agcagttgag gcatgtaaga agttaggaaa
      781 agatgtggat gtgattcagt tagaagatag aatattgcca caagtatttg ataaggaaat
      841 tacggatgta ttagaagaag aaataaatag gcataatgtt aatttgcact tggatgaagt
      901 ggttgtagaa ctttatggag aagataaagt agaaaaagtt ataacaaaca aaggagaaat
      961 agatacagat gtagtaataa ttgctactgg tgttagacca aacaccgagt tcttgtcaag
     1021 tactaatata aaaatgctaa aaaatggagc tataatagtt gatgaatatg ggagaacctc
     1081 tgtggaggat atatactctg ctggagactg tgcaactata aagaatatag taagcaatga
     1141 aaatgtatat gtgcctttag ctactggagc taataaattg ggtaggatag taggagaaaa
     1201 tctagcagga cgagaagtat cttatcaagg ctcactatct tcaagttgta taaaaattat
     1261 ggatatggaa gcagcatcaa ctggaattac agaaagaaaa gctaaagatt taggtataaa
     1321 tgttaaatct aaatttattt ctgactataa tcaaactcat tattatccag gaagaaataa
     1381 aatatatgta aagcttattt atgatgcaga tacaaaagtt gttttaggtg gacaagtggc
     1441 tggttttaag gatgctgttc aaagagcaaa tgtattggca gctgctatat ttgggaaaat
     1501 gactacaagt caacttggaa tgttagattt atgttatgct ccaccatttg caagaacctg
     1561 ggatgtttta aatgtagctg ggaatgtatc taaataatgt ttagattata gctaatataa
     1621 ttgaaaagca attaaaggaa tgaatttacc taaaatattt ttgtcatggt aaaattcatt
     1681 cctaaatttt taattcaaat atttaaaagc aagtattata ttaaattatt catcaaaatt
     1741 taaatgcaag cttaatctat cttctccaag aatagtattt tcaaaaacat tagtagcatt
     1801 ttctaaatat atactaggtg tatctaaaga agggctaaaa tgagtcagta aaagttgttt
     1861 gacatttcct agttttgcaa gattagcagc ttctctaaaa gtcatatgtt tgtttctaac
     1921 agcttttgat atatctaagt catcaccata catggcttca catataaata aatcactatt
     1981 ttttatgaaa tctggtattg attctatagg tcttgtatcg gttgtaagac ttatcttaac
     2041 tccttttctc aattccccta agaccatatc tggagtatat ttttttgaat ctaaaactat
     2101 attttggcct tcttggagct tattccataa tattttagga acttcatttt tagttgcctt
     2161 atctatatca aatttagggt ttcttctaaa attaaaactg tatcctaagc actctataga
     2221 gtgttctaaa ctgattgttg aaatttctaa attccttaaa actttattat ctaaagaaaa
     2281 actgccttga ggattttcta taatttttaa agtatatggt atatattcta ctaaatttct
     2341 catagaacgt atacaatcta ctattccaat tggaccaact atagttaaat cacttgtacg
     2401 accactattt ccaattgttg ataaaagccc taaaagtcca aatatatggt caccatgtaa
     2461 atgggttata cagattaagt ctatatcttt aaagccacat ttttttagct tcatagatac
     2521 ctgagtacct tcaccacagt caaccaatat ttttctacct ttatagttta taaatacaga
     2581 ggatagaaat ctgtttggca taggtacatt tccaccacat ccaagtaaag ttaaatctat
     2641 cattttttcc cctttctaaa attaacacaa atctgttgtt tgtttttatt atgatatcct
     2701 attataatta ttttaacatt aaatataaca ataaatgtgc acttgagtca agaaatattg
     2761 tcaaatagag tttttatata acaatgtata aaaatttgtg tagcaaaaag taaaaattat
     2821 atttaggaga atttaaagga ggagaaatta atgaaagtat taaagaaaat taccattttg
     2881 tcattgatta tattaacttt agttggttgt agtaacggag aagataaaaa agtggttgaa
     2941 ggttctaaga aaattcaaaa ttctaaaaaa aatgaacagc ctataatagt acataaagat
     3001 gcaaatatat tgaaagacag gtatgagcca ccaaaaggat ttaaaagagt caatgtagaa
     3061 aaaggaagtt ttgaatcatt tcttaggaat actaagctaa aaaattatgg agagaaagta
     3121 aagtactatg atggaagaac taaggaatca aaaggtattt atgatagtgt atttgatgtt
     3181 gatattggta atagagattt acatcaatgt gcagacgcaa ttatgcttat gagagcagaa
     3241 tacttgtatc agaataaact atatgatgaa ataagttttg attttgttga tggttttaag
     3301 gcgaagtatt caaaatgggt acaaggatat agaatttcag taaacgatag tggttcaagc
     3361 tggtataaag caactgaata ttcaacatct tatgagagct tcagaaagtt tatggatata
     3421 gtgtttgcct attcaggaac gttatctctt gaaaaagaat tggagcctgt aaaagttgaa
     3481 gatatgaaaa taggagatgt atttatagtt ggtggaagtc caggacatgc aagtataatt
     3541 atggatatgg cagaaaatga gaagactgga gaaaaggtat ttatgatagc ccaaagctat
     3601 atgcctgcac aacaaactca acttcttatc aatcgtaaaa atccaaattt gagtccttgg
     3661 tatagtttag attttgaagg tgatttgaca atacctcaat ggacttttaa aagagaccag
     3721 ttgaaaagat tttaaaataa taattttata taataaaaga aaaatataga tgtgaaaaaa
     3781 tgtaattaga ttaaaactaa ttggatatgg acaaaattaa attgatagat aaatattaaa
     3841 aattatacta attaatttta ctatagttta atatttatct aaaaagttta atgtaaaatt
     3901 tatattttac attaaagcta aaactaattt gaggaggcta atttatattg aagattagaa
     3961 gatatttaat tttggtaatt ataattctaa ttacaacaag tcagagtttt atttcttatg
     4021 ggctagatag atttgaagaa ataactattt ttcacactaa tgatatacat gggagatatg
     4081 ctgaaggtga tgagcatata caaataggga atctagctac attgaaaaaa gaaactccaa
     4141 actcaatttt agtagatgca ggagattgtt tgcatgggct tccaatagta aatatgggta
     4201 aagggaaaaa tgcagtggaa ttgattaaat gtgctggata tgactatatt actcctggaa
     4261 atcatgactt taattatgga aaagatagac tgtttgagtt atctaaaagt gctaatttag
     4321 gagagaatac tttaaaattt ttatcatcaa atatttttga aaatgggaga aaagtttttg
     4381 atagtaatga tataaaagaa ataaatggag taaaaatagg attttttgga ctctcaaccc
     4441 aggaaacaaa gagcaaaact ggatttaaaa atgtggaggg gttagagttt aaaaacccta
     4501 tagaaagtgc aacagagcaa gtaagtgaat taaaagaaaa aggtgcagat gtaatagttg
     4561 caatatgtca tattggtaca aacactttaa gtaagccaaa tagtatagat atagcaaata
     4621 aggttcaagg aattgatttg attattgatg ggcacagcca cactaagttt gaaaatggta
     4681 aacaagttgg tgatacattg attgtaagta cagggcaata tttagaaaat attggcgagg
     4741 taaaactaac cttggatact gatgatgtag gaaatataaa aatagaaaat aaatcagcta
     4801 gattgattgg aaaagaagaa gcattaaagt ataagcttga ctccagtgta agtaaaaagg
     4861 ttgacgaaat aaaagagcaa caagaaaaaa tattagataa agttataggt gtaactacaa
     4921 atactctaga tggttcatat gaaaatgtaa ggacaaaaga aactaattta ggtaatttaa
     4981 taagtgacat tttactagat aaaactaagg ctgatatttc tttgtttaat ggagggaata
     5041 ttagagatac gatagaaaaa ggcaatataa caataagaga tattgtagat gtattccctt
     5101 ttagcaatac aatagttacc aaagaactta caggagcaca gattaaagat gtattggagc
     5161 atggagtaaa aatttatcca gaaaaaagtt cggctttttt acaagtagga ggaatttcat
     5221 attactttaa tcctaaacaa aaagaaggtg aaaggataac aagtattcaa aaagaaggaa
     5281 agccattaga tttagataaa aagtatgtag ttgcaacgaa tgattatata gcttcaggtg
     5341 gagatgaatt tccttgtttt tcaaaagagc caatcttaaa ggaatttgga aacttagaga
     5401 gtgcagttac tgaatatata gagtacaaaa gagaaatatc aaaaaatgta gatggaagaa
     5461 ttggtattaa atctaaagat gagttaataa gtgagaatca agaaagtgat ttgatattaa
     5521 gtgaaaataa agaaagtatc aatgataatg aagagaatct atctaataat ataaaaagtt
     5581 ctgatagtga taaaaataaa atcttagata aaacaaggac tagagaaagt tctaatctag
     5641 gaatatctaa aagagaagct agagaaaaat ctcctaaaac aggagattta ggtttttcaa
     5701 atagtataat tatatttata gtttcaagta cattaatatg cttattgaat tttaatcaaa
     5761 aagagttaaa aaataaaaag tccaagtaaa agttaatgag caaaagttag tagatagtaa
     5821 tttaatttta gataatagta aacagcaaag gaaattagtt atataaatac taatttcctt
     5881 tgtttttatg ctttaacagg attactttta attaaagttg tattttatga aaaataatta
     5941 cactaaatag tacggtacta tgagtatttt tagtttttat tcaagtaaat gaaatcatat
     6001 taacaacctc tgatatattt ataaattgat aaaaaaacgg ttttactagt atgatataat
     6061 ttagctagta cgtatgataa tggaggacat gtggatgaat aatatattac ttttagaaga
     6121 tgacaaatct ttaaatagag gaattagttt taaattaaaa aaagaaggat acaatgtttt
     6181 tagtgcattt agtatagaag aagcaaaatc tattttcgca aaagaagaga tatgcttaat
     6241 tatttctgat ataggtctgc ctgatggtag tggttttgac ttttgtgagg aggttagaaa
     6301 gaaaagtgat gtttacataa taatgttgac agctcttgat gaagaggtag atattgtcac
     6361 aggatatgat ttaggtgcag atgattatat cacaaagcct tttagtttga tggtattaat
     6421 atcaaaagtg aatgcgctta tgaaaagggt aaatacagta aaaaattata ctttattggt
     6481 ttgtgatgat ttattctttt actatataga aaataaattg attgtaagag cagataataa
     6541 ggaagaagag ataattttaa gtaaaacaga gactaaattg ttaaaatatt tgatggaaaa
     6601 ttctatgcag acattgacta aagagcaact gttagaatct ttgtgggata gtagtggtaa
     6661 ttttgtagat gataatacga ttgcagtaaa tataagaaga cttagacaaa aggttgaaaa
     6721 aaatccatct gcacctaagt acataaaaac agtaagaggg gttggatata tatggggaga
     6781 aaggagtata aaaaaatgtt aaaaaatatg aacctcaaag atggtttaag tgtgaaagat
     6841 gattatataa tgagaagagt tattgtatat tctattttat ctatactaat agtaaccatt
     6901 atatttttac tttttgaata tagtagatta tataaactgt atgaacatca aataaaaaat
     6961 actcaaaata tcgctggaat attgattgaa aaatacccta atgatgaagt agatatagta
     7021 aagtctattt ataattctaa ttatagtaag gtgcctattg gagaaaaggc atttaagaaa
     7081 tttggctatg gattggaaaa taaaatgtca aatgataaaa attttagctt atacttaaaa
     7141 tcttttttta aggaatcatt ttttattttt ttaatgatgt ttatagtagt tataggtata
     7201 atatattatt ttataagata tataaataaa aatttgagta aaatctattt tatagtagat
     7261 aagatggcac aagagaacta cttgaaagac actactgtat cagaagagac tggttatcta
     7321 gtagaatata atgagtattt taaagaaggt acatttagta agataaataa ctgtctatat
     7381 gagcttaata gaagtttaaa gataaagttt attaagttgg aaaaagaaaa agaaagtgta
     7441 aaatcattag taacagatat ttcacatcaa ttaaaaactc ctttagcatc cttaaaatta
     7501 tacaatacat tacttataga agaggaatta gaagaagatg aagccaacga gttcttatta
     7561 accaacaaag cttctataaa taaattagaa aatttaataa attctttagt gaacatatca
     7621 aggttagaaa ttagtatgat aaacataaaa aaagaagata atgatataaa aaatactatt
     7681 ctagatgcag taaaaagtgt cacccctaaa gctaagaata aaagcataac attaaattta
     7741 aatgaatttg acagtagaat tgtaccacat gataaaaagt ggactgaaga gagtattttt
     7801 aatgtattag ataacgctgt aaaatataca cagataaatg gagaaataaa tatatctgta
     7861 gaagaaactg ctaattactt taaaattata atagaggata atggtatagg aattgaaaaa
     7921 aatgagttta ataatatctt taaacgtttt tatagaggtt ctgctgatga aattgaatct
     7981 gtagaaggta gtggggtagg attgtatttg agtaggaaaa ttttggaaga acaaggtgga
     8041 aatattatag tttcttcaaa agtagggata gggagcaagt tctctttgtt tcttacaact
     8101 atgtaatatt aaatgaaagg aaaatgtaag cctctacatt tattatataa gtataaacaa
     8161 ctccagaaat aagaaattaa taaaatatgg ggtaatataa cttagaatga gaggataatt
     8221 ttatgaaaat actatatact gaaaatttat caaagcatta tggaaaagga gaaagcttag
     8281 taaaagcttt ggataatgtt aacttagaaa taaatgaagg tgaatttgtt gcaataatag
     8341 gaaaatcagg ttctggaaaa agtacacttt tgcatatgat tggaggtctt gatataccta
     8401 catctgggaa ggtgtatata gataataaaa atatatttac attaaaagaa gaagaacttg
     8461 ctgtgtttag aagaagaaaa atagggttta tatttcaaag ctataattta attccatctc
     8521 ttaatgtatg ggaaaatgta gtgttaccaa ttggtctaga tggtagagaa gtagatgaga
     8581 cctttataaa agaactttta aaatcattag ggcttgaaaa taaacgcgac gttttaccaa
     8641 atacattatc aggtggtcaa caacaaagag tagcaatagc aagagcatta gctacaagac
     8701 cagctataat acttgctgat gaaccaactg gaaacctaga ttctaaaaca tctgatgagg
     8761 ttatgagtat attaaaatct atgtctaaga agtatagtca aactctagtt atgataactc
     8821 atgatgacag tatagcccaa atggctgata gagttatatt catagaagat ggcaaggtat
     8881 taaaggttgg tgacaaaaat gattaatact actaaaatag caaaaaataa tctaaagcaa
     8941 aataaatcaa aaagtattct aataataatc actataatac tttctacctt aatgctttct
     9001 tctattggaa tttatattgt taatgcagga gcatatcaaa aagaaaatac tataaagtat
     9061 tcaggtaatt atcaaggtat attagcaaat gtggatgaaa aacaagctga tatattgagt
     9121 aatcatgcag atgttgaatt aactggtgaa atgaatggag taggtgttga aaaactggaa
     9181 gatgattcaa atatttctct ggcatatatg aatgaagatg ctttgaaatt aaatagtttt
     9241 gaatttgtca aaggaaaatt accaacaaaa gagaatgaaa ttgtgttaga tagtggagct
     9301 ttaaaagcac ttggttataa aaataaagac ttaggtgaaa ggataaaaat atcttataat
     9361 gactacaaaa atgataaaaa aattgaaaaa gagttcatta taagtggaat cttaaaaact
     9421 agcgaaatat ctgaagcagg taaatattat tatgctatta tatcagaatc gtatatgaga
     9481 aatactagaa atatgtcaca agaagatttt aatatttatg taaaatttaa tgataaaagt
     9541 aatttatcaa tagagcaaat aaaagaaaaa ttagataaaa ttgctaatga tataggattg
     9601 gatactatta atactgctgt aaatgaaaat tatataaatg ctttaaaacc agatatggaa
     9661 acgattatgg gtggagtttt tgtaggatta gtaattgtat tatctagtat tttagtaata
     9721 tacaatatat tttatatttc aatagttaca aaagttcaag aatttggaaa gcttagagca
     9781 attggtgcaa ctaaaaaaca aataaaaaat atagtattta aagaaggatt tatattagca
     9841 ggaatatcaa taccaatagg aataatttta ggttatgttc ttgcaaacat tataataaaa
     9901 tcatttatga acatagatgc aaaatcatca caattaccag ttatattatt ggttgcagta
     9961 ataagtttta tatctgttgt tttatcgctt ttaaaaccaa tgaaagtggc atcaaaagta
    10021 tctattgttg atgcagtaag atattctgga aataaaataa gcaataaaaa taagagaaaa
    10081 ggttataaaa atataaattt aaatagatta tcacatgcca atttagaaag aaataaaaaa
    10141 cgtacttata tgacattggc atcacttata cttagtggga ctatatttat aacagtatcg
    10201 actgctttag aaagttttga tgctgagaag atggcaagag aacactttcc ttatgatata
    10261 gaagttagat tatctggtta tgaaatgaac agtgataaaa atcctaaaaa taatttgaat
    10321 attttacaaa tggataatcc tctaagtaaa gattttttta atcaaataaa gaacatagaa
    10381 ggaattaaaa gaatagagtc tgcaagaagt gtaaaaattg gtatggagga ctatgatgtt
    10441 gaatttaaat atgacctttt acaaagtata aacgaaaatg atgtaaaatc attaagcaag
    10501 aatctaatag atggaaaaat aaatcttgaa agattgcaaa ctggagatga aatagtaatt
    10561 actcatgttg atacagcaaa agaaatggga gtaaaagcag gagataaaat aagattaact
    10621 ttatatgatg gagataaaaa gataaaaaaa gaatttaaag ttcaagcaat agctatggga
    10681 gttccaagtt ttggtatagg aaaggatttt atagatagaa ccttgaaata cgattcaaca
    10741 agtagtttag gaatatatac aaaagaagga aagtatcaag aagtaaaaga tagtataaag
    10801 aaaattgcta aatctaatgg atttttagag acagatttta tagatagtag aattgaatca
    10861 aataaggcta ctatttcgtt tataaaaata atgggttata cattaacagg cataataggt
    10921 gtgattggat ttatgaatct tgtaaacaca atgataacta gcatagttac tagaaagaaa
    10981 gagcttggga tgcttcaagc gattggtctt actaataaac aattggtgaa aatgcttaac
    11041 tcagaggcaa tatcttacac ttctggaatg atgataggaa gtattttatt tggaggtata
    11101 ttgggatata ttgctgttat ggttctaaag aagactggat tatcttatgc aacgtattct
    11161 ttgccaatag taccaatctt gttgatgatt gtatgtattc ttatagctca atttataaca
    11221 acttatttga taggacgaag ttttaataaa gaatctctta ttgatagagt aagatacagt
    11281 gaataaaata taaatattta tgtatttatg gttagtttaa ctagcaaaaa ccatataaaa
    11341 aagatggtta aatagattca taattaaata ggttaaagta tagttacaga gcaaacaatt
    11401 taaactgatg tttgctctgt attttattgt gcaataaata atatatataa tagatttata
    11461 ttatttatgt cagaaaatgt attggtacaa ttctaaaaaa gtataaaatt gtaccatata
    11521 aaatgaaaat tttgtataat aatcgtatag aaaataaata ggagctgata tatatgaatg
    11581 agaagattaa ttatgcacca ggaccaactg aaactaggga aaatgtaaga ttagcaagag
    11641 cagaaaaaac aacaaatcca gatatagata ttgattttgt tgaattttat aaaaaggcat
    11701 gcgagaagtt tggcaatata gttggcacaa aaaacgatgt ttacatattg agtggtgaag
    11761 ggatattagg acttgaagca gcatgtgcat cacttacaga aaagggagat agagtacttg
    11821 taattgataa tggtatatat ggagaaggtt ttaaagattt tgtaacaatg tatggtgggg
    11881 agtatgtgct tttttcaagt gaatatacta aaagtataga tattgatgaa ttgaaagcat
    11941 ttttagataa ggatagtaac tttaaatatg caacagtagt gcattgtgat actccaactg
    12001 gtgtattaaa tgatgtaagc aaaatatgtc cattacttaa ggaatatgga atattaactg
    12061 tagttgattc tgtagcaggt atggtaggag aaagattaag tgtagatgaa tctaaaatag
    12121 acataatatt aggcggttcg caaaaagcta tttctgcacc agcaggtctt actatagttg
    12181 gaataagtca agatgcaaaa aattgtataa aaaatagaaa aactgatgta ataggttttt
    12241 attgtaattt aaatatatgg gaagactatt atgaaaaaaa atacttccct tacacaatgc
    12301 caataagtga cataatgggg cttgattaaa aaatagctat gcacact
//
LOCUS       DAFNNB010000116        12261 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0117, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000116 DAFNNB010000000
VERSION     DAFNNB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12261
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0117"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            537..1598
                     /locus_tag="L3M60_002649"
     CDS             537..1598
                     /locus_tag="L3M60_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HBN6227296.1"
                     /translation="MRDFLKNYKSSIILLACTIIGAIVGLVLGPKASILKPFGDLFIN
                     ALLMIVVPLVFLSVSSGVASIKNMERFGKIMKSTAIVFMGTTLVIAFLGITSALLFNP
                     SKGLDVSSVKSLMVAQDAETLESKGTLLDQVVSSITVKNFGDLFSSSNLLQLVIFAVL
                     VGTAIAMMGDRGNTIRKLLSEGTEAILKVVSIVMLYAPIGLGCYTATIIGELGSKIIQ
                     GYVRVFVIYSLTAVMYFLLFYSLYAFLSAGKQGFRQFWRNAIKPIATALATSSSSACI
                     PSNLEASKNIGVPDDISETVIPLGINIHKDGSVLAVIIKVAFLSGILGSGMANPATIG
                     QLFITVFVVGVFMAPVAGG"
     gene            1611..1808
                     /locus_tag="L3M60_002650"
     CDS             1611..1808
                     /locus_tag="L3M60_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBN6227297.1"
                     /translation="MLVVSIFGFPVEAIPLLTVITTITDIPNTILNTTGNTVSSMLVS
                     RLVEGKDWLIDKTAITTKKIS"
     gene            complement(1958..2662)
                     /locus_tag="L3M60_002651"
     CDS             complement(1958..2662)
                     /locus_tag="L3M60_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HBN6227298.1"
                     /translation="MQQGSDYLKKILEKHIDLLKKIPLFEGIKSDELEAMLECLGVVD
                     KIYPKDSIIFSVGSEVTSIGIMLKGSAHIIKEDIEGNRNIVAELLPGDLFGEVFACTR
                     LHKSNVTVTTTSSCEILFIKFKSVTGICSSTCVFHNRLVENMLQLIAEKNILLHNKIE
                     LLSRKTTREKLLMYFSKQIEQTGSNKFTIPFSRNEMADFLCVDRSSMSRELGKMRDEG
                     LLSFYKNKFEIFCFQK"
     gene            2823..3548
                     /locus_tag="L3M60_002652"
     CDS             2823..3548
                     /locus_tag="L3M60_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6227299.1"
                     /translation="MMRKIIKIDEKKCNGCGLCVEACHEEAIGMVNGKAKLLRDDYCD
                     GLGDCLPTCPTNAISFEYREAAEYDEAAVKANMEAKKTQNKNLACGCPGSQSKSINRE
                     GSNNASISNDVVETKGSQLNQWPVQIKLVPTNASYLKNASLLIAADCTAYAYGNFHNK
                     FMKNKVTLIGCPKLDEIDYTEKLTEILKENDIKNIVVTRMEVPCCGGIVNAVKTALQN
                     SGKMIPWQIVTISVDGKIVDGEI"
     gene            3607..5199
                     /gene="hcp"
                     /locus_tag="L3M60_002653"
                     /gene_synonym="priS"
     CDS             3607..5199
                     /gene="hcp"
                     /locus_tag="L3M60_002653"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBN6227300.1"
                     /translation="MEKMFCFQCEQTAGGKGCTINGVCGKKGDTANLQDELTGVLIGL
                     SRATIGNKNRPTSKTDKIMIEGLFTTITNVSFDDEAIKRQIEKTEIEKSKIVPRCSDC
                     STTCNRNDNYDMKDLWNDNEDIRSLKSLILFGLRGMAAYAYHAMVLGYTDKDVNEFFY
                     EGLIAVGGDLSIDELLGLVMKTGEINLKCMELLDRANTETYGTPEPTQVSMKIEKGPF
                     IVVTGHDLYDLHKLLEQTKDKGINIYTHGELLPAHAYPKLKEYKHLKGNFGTAWQNQQ
                     KEFDNIPAPILFTTNCLMPVKDSYRDRVFTTEVVAYPGMVHIDEDKDFSSVIEKALEL
                     GGYKEDVHMTGINGGDTLTTGFARGTVSSVQDKVVDAVKSGDIRHFLLVGGCDGAKPG
                     RNYYTEFVKQAPKDTVILTLACGKYRFNDLDLGDIGGLPRLMDMGQCNDAYSAIKVAV
                     SLANAFECGVNDLPLSMVLSWYEQKAVCILLTLLHLGIKNIYIGPSLPAFLSKNVLDI
                     LVDKFALTPISTPEEDMKKILG"
     gene            5577..6482
                     /locus_tag="L3M60_002654"
     CDS             5577..6482
                     /locus_tag="L3M60_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBN6227301.1"
                     /translation="MKNSNFNILAFDGGGLKGALSISILERIVKEYPNLLNNINMFGG
                     TSTGSLIALGLAYGVSPKEIKELYSIENSKHIFNKSYTEMLRPKYENKNLKEVLLRIF
                     PEELELKDLNKLVMIPSFYIGNEENAWKPVFYNNMPNSFTKTSKVVDVAMASSAAPVF
                     FPTYNRHVDGGIIATDPSLACIIHAIDSGFKLKNTRLFSIGTGYVYNSIKADTTEWGA
                     IDWIINKEPDLPIISITLEGNSQMSQLFSQKLLGDNYYRLNPKMEKDVAMDDCDALEY
                     LMSLGENCDIKDAFGWIKKKSFYRN"
     gene            6606..7559
                     /gene="msrA"
                     /locus_tag="L3M60_002655"
     CDS             6606..7559
                     /gene="msrA"
                     /locus_tag="L3M60_002655"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBN6227302.1"
                     /translation="MVKLATFAGGCFWCMVKPFDKYDGVIKVISGYTGGHIENPTYQE
                     VCSGTTGHVEAIQITYDDDIISYDELLNIFWKQINPTDDGGQFGDRGTQYKTAIFYHD
                     EEQKNIALESKEHLENSKIFDSKIVTDIIPATKFYEAEENHQDYYKKRPLNYNMYYKG
                     SGRYNFVKSHWDGNNINREELKKTLTPIQFEVTQNDMTEVPFENEYYDNNKEGIYVDI
                     VSGEVLFSSKDKFDSGCGWPSFSKPVIDKSIIERSDFSQGMYRVEVRSAKGNSHLGHV
                     FDDGPKELGGLRYCINSASLKFIPKEDMETLGYGEYLKLFE"
     gene            7712..8842
                     /locus_tag="L3M60_002656"
     CDS             7712..8842
                     /locus_tag="L3M60_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227303.1"
                     /translation="MNLKDFNGKKLKSARTFRAKTIEQLSKETKINKKDLKAFEENKY
                     VPNIENTLKLSNILNFPKEYFYKNENINVVVEDSHFNPQSKLPRVEEISYREKIIIIH
                     KIYSFMENYIKFPKENLPSRKVMSDININDIESLAYKTREFWDLNSTPIVNMVNLLES
                     KGIIISGMNVDRKWATIFTQKQRISKESKYIISLGNDKKSASIRNYTLACELGYIISS
                     ELRIPNKQFSEDEYACAFLLPKDIFLEDLIHPEDLDYYVELKKKWIVPISAMIFRAYN
                     LGKINYKKYNYLVNEMDKKGWLIEEPLDKMKGSSPTYLKRAVELLIENKIMSVNSIVS
                     SLDEFGINLYPEDLELLMGLKKGLLSQEVNKKSKVIKFDSKK"
     gene            complement(8959..9927)
                     /locus_tag="L3M60_002657"
     CDS             complement(8959..9927)
                     /locus_tag="L3M60_002657"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HBN6227304.1"
                     /translation="MAIKPRKISIVGSGHVGSHCGFSLITQGVCDELFMIDIDESKSK
                     AQALDLADAVSYLPHKVHIEKGTFSDCKDSDIVVISVADSSEGPLRRQNTTRLDLLRP
                     TIGMIKSIVKPIVDSGFDGIFIVISNPVDVVTNYIWEKSGFPKNKVIGTGTALDSTRL
                     RRLLSEETGIAQQSIQAYSMGEHGDSQMVPWSHVSIGGKPILDMIKDNPNTYSNLDLP
                     SIVEKNKKTGINIINGKDCTEFGIGTALVEIVKAILHNEKKILPVSTLLENQYNERNV
                     FASVPCVIGKDGIEEIIEINMTEDEQNEFNKSCSVLREYIALSKTL"
     gene            10501..11700
                     /locus_tag="L3M60_002658"
     CDS             10501..11700
                     /locus_tag="L3M60_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein AlaX-L"
                     /protein_id="HBN6227305.1"
                     /translation="MEKLYYTDQYIKDFTAEIVEIKEVENKFHVLLDKTAFFPGGGGQ
                     FADLGKIDNYDVISVYEEGKKVYHVLDKKPIKIHRVKCSIDWNRREDGMHQHFGQHVL
                     SGCFFKTFNANTTGFHLGSEYSTVDIEGNLDEDKIREIELLSNEIIRQNIQVETLLPT
                     KKELKKIWLRRDLPNTDEEIRVVKIGDLDTNACCGVHPKATGELRMIKIKKWEKHKSS
                     TRIEFLAGKRAVDDILKRDIYLTKICRYLSCNEEDAINGIVNLNSKVEEVLIKKRRLE
                     EVVSTYQVKEMIEKANKIGNISVVKKIYEDEDLKYVNKIANKITEHENNIALLAVKFN
                     DKINLVFACSKNLKDINMGVLLKDAISLIDGKGGGSKILAQGGGKNNGNLEATLDYAF
                     MKIEKTL"
     gene            11752..12252
                     /locus_tag="L3M60_002659"
     CDS             11752..12252
                     /locus_tag="L3M60_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227306.1"
                     /translation="MEQQKDYIELYENEELIEIREACMNDLDTIAKFNYNLAKETEGK
                     ELDMDVLTKGVKALLLDERKGKYHVYTVFDKVVAQIMYTYEWSDWRNGNFLWIQSVYV
                     DKEYRRKGIFNYLFNYIKNICDKDENIVGMRLYVEKENMSAKATYESLNMYECDYNMY
                     EYEVMR"
BASE COUNT         4822 a         1301 c         2083 g         4055 t
ORIGIN      
        1 agttagataa taaatttaat tggagtatct ttttattatt agatttatta tttaatttaa
       61 aattagaaaa gtagctattt gaaaattact tttaaaagta tatcaaaata tcaattacat
      121 gaaaaaataa tatttgacaa aatatcatat gtgtttacaa aattaacttg atggaatgat
      181 aaagtatagg aattacaatg agtatactaa aactttatat taaagtacaa attattaaat
      241 ttgaaaataa ttaagtaata ttatatatat ttttttatat tgtttgtttt attaaaatca
      301 atctagaaaa aaagttttaa aagcagaatt ttattaatat ttatatatag gtatgaacta
      361 ttaaaaaaag attaaagata caaaaaaact atagaaattg acatatattc cagtatttgc
      421 taaagtagct gttggggaaa attacaaaaa ctattaatgc gcattagata atttacttta
      481 atttttttag ggtttggggt aatttataat ataaaatatt agaaaggaaa atataaatga
      541 gggacttttt aaaaaattat aagtcatcaa ttatactttt agcatgtact ataatagggg
      601 cgatagtagg attagtactt ggtccaaaag cttcaatact aaaaccattt ggagatttat
      661 tcataaatgc tctcttaatg atagtagtac cattagtttt tcttagtgtt tcttcaggtg
      721 ttgctagtat aaaaaatatg gaacgctttg gaaaaataat gaagagtaca gctatagtat
      781 ttatgggaac aacacttgta attgcatttt taggtattac atctgcatta ttatttaatc
      841 catctaaagg attagatgta tcaagtgtaa aaagtttaat ggtggctcaa gatgcagaaa
      901 ctttagaatc aaaaggaaca ttattagacc aagtagtatc aagtattaca gttaaaaatt
      961 ttggagattt attttccagt agcaatttac tacaattagt tatatttgca gttttagttg
     1021 gaacagcaat agctatgatg ggagataggg gaaatacaat aagaaagctt ttatcagaag
     1081 gaacggaagc aatattaaaa gtagtaagta tagtcatgct ttatgctcct atagggctag
     1141 gatgttatac agctacaata attggggaac ttggaagcaa aataattcaa ggatatgtaa
     1201 gggtgtttgt aatttattcg ctaacagcag tcatgtattt cttattattt tacagtctat
     1261 atgctttttt atctgcagga aaacaagggt ttagacaatt ctggagaaat gctataaaac
     1321 caatagcaac ggcactagct acttcttcaa gttcagcatg tataccatca aatttagaag
     1381 ctagtaaaaa tataggagtt cctgatgata tatcagaaac agttatacct cttggaataa
     1441 atatacataa agatgggtca gttttagcag taataataaa agtggcattt ttatcaggta
     1501 ttttaggtag tggaatggca aatccagcaa cgataggtca attatttata actgtatttg
     1561 ttgttggagt atttatggct cctgtagcag gtggctgact tattggtgag atgctcgtgg
     1621 ttagcatctt cggttttcca gtggaggcaa ttccattgtt aacagttata actactataa
     1681 ctgatattcc taatactata ttaaatacaa ctggaaatac agtatcatca atgttagttt
     1741 caagattagt agaaggaaaa gattggctta tagacaaaac agcaattact acaaagaaaa
     1801 taagctaatt atatacttaa ttaataaagt taatagatat cttaatttta ataaattaga
     1861 tatctattaa ctttttaaat aaaaatataa atttaagatt caaatatgat atttataatt
     1921 tgaattaact aatataatat atgtttaaat atctagttta tttttgaaag caaaagattt
     1981 caaacttatt tttataaaaa gataacaagc cttcatcccg catcttacca agttctcttg
     2041 acatagaact tctgtcaaca cacaaaaaat cagccatttc atttcttgaa aaagggattg
     2101 taaacttgtt gctacctgtt tgttctatct gctttgaaaa atacataagc aatttttcac
     2161 gagttgtttt acgagaaagc aattctattt tattgtggag taaaatattt ttttcggcta
     2221 ttaattgtag catgttttca acaagtctat tatgaaatac acatgtagat gaacaaatac
     2281 cagttacact tttaaacttt ataaataaaa tttcacaaga agatgttgtg gtcactgtga
     2341 cattactttt atgtagtcta gtacatgcaa acacctctcc aaataaatct ccaggtaaga
     2401 gttcagctac gatatttcta ttaccttcaa tatcttcctt tattatatgt gcacttcctt
     2461 taagcataat tcctattgaa gtaacctcac ttccaacaga gaatattatg ctatcttttg
     2521 gataaatctt gtctacaaca cctaaacatt ctaacattgc ttcaagttca tcacttttaa
     2581 ttccttcaaa tagaggaatt ttttttagta ggtctatgtg tttttctaaa atttttttca
     2641 aataatcact tccttgttgc atatgcaacc gataatttgt ttttattgta ctatactaag
     2701 tttataaaag caaggagata aaaatactac tataagaaat ttgaaataaa aagatgtagt
     2761 cttaaaaata aatatataag aagtaaatat ttaaaattaa aaaaatttat gaggtgatat
     2821 aaatgatgag aaaaataatt aaaattgatg agaaaaaatg taatggttgt ggactatgtg
     2881 ttgaagcttg tcatgaagaa gctataggaa tggtaaatgg aaaagcaaaa cttttaagag
     2941 atgactattg tgatgggctg ggagattgcc taccaacttg tccaacgaat gcaatttcat
     3001 ttgagtatag agaagcagca gaatatgatg aagcagcagt aaaagcgaat atggaagcaa
     3061 aaaaaacaca aaataaaaat ttagcatgtg gatgtccagg ttctcagtct aagtccataa
     3121 atagagaggg ttcaaataat gcatctataa gcaatgacgt agtagagaca aaaggttctc
     3181 aacttaacca atggccagtt caaataaaat tagtaccaac taatgcatct tatttaaaga
     3241 atgcaagcct tttaatagca gcagattgta cagcatatgc atatggaaac tttcataata
     3301 aatttatgaa aaataaggtt acattaatcg gatgccctaa acttgatgaa attgactata
     3361 ctgaaaaact tacagagatt ttaaaagaaa atgatattaa aaatatagta gtaacacgta
     3421 tggaagttcc ttgctgtggt ggtatagtaa atgctgttaa gacagctcta caaaatagtg
     3481 gaaaaatgat accatggcaa atagttacta tatctgtaga tggaaaaatt gtggatggag
     3541 aaatttaatt aagtgaatta cagaaaaatt taataaattt taataaacaa aagggaggaa
     3601 taaaaaatgg aaaaaatgtt ttgttttcaa tgtgaacaaa cagcaggagg taaaggttgt
     3661 actataaatg gtgtttgtgg taaaaagggg gatacagcta acttacaaga cgaacttaca
     3721 ggagtgttga ttggtctttc aagagcaact attggaaata agaatagacc aacaagtaaa
     3781 acagataaaa ttatgataga ggggttattc actactataa caaatgtaag ttttgatgat
     3841 gaggctataa aaagacaaat tgaaaaaaca gagatagaaa aaagtaaaat tgtacctaga
     3901 tgttcagatt gctcaacaac ttgtaataga aatgataact atgatatgaa agatttatgg
     3961 aatgataatg aagatatacg ttcattaaaa tcattaatat tatttggatt aagaggtatg
     4021 gctgcctatg catatcatgc catggtacta ggttatacgg acaaagacgt aaatgagttt
     4081 ttctatgaag gattaatagc agttggagga gacttgtcta ttgacgaact tttaggttta
     4141 gttatgaaaa ctggagaaat aaacttaaaa tgtatggaat tgcttgatag agcaaatact
     4201 gaaacttatg gaacgccaga accgactcag gtatctatga aaatcgagaa aggaccattt
     4261 attgtagtta caggacatga cctttatgat ttacacaaat tattagaaca aacaaaagat
     4321 aagggtataa atatatatac tcatggtgag ttattaccag cacatgcata cccaaaatta
     4381 aaagaatata aacatctaaa aggtaatttt ggtacagcat ggcaaaatca acaaaaagaa
     4441 tttgataata ttcctgctcc aatactattt acaacaaatt gtttaatgcc tgttaaagat
     4501 agttatagag atagagtatt tactacagaa gtagttgctt atccaggtat ggttcatatt
     4561 gatgaagaca aagatttttc tagtgtaata gaaaaagccc ttgaacttgg aggatataaa
     4621 gaagatgttc atatgactgg tattaatgga ggagatacat taactactgg ttttgcaaga
     4681 ggtacagttt catctgtaca agataaagta gtagatgctg taaagagtgg agatataaga
     4741 cactttttat tggttggagg ttgtgatggt gcaaaacctg gaagaaatta ttacactgaa
     4801 tttgtaaaac aagctcctaa agatactgtt atattgacac ttgcatgtgg aaaatataga
     4861 tttaatgatt tagaccttgg agatataggt ggcttacctc gtttaatgga tatggggcaa
     4921 tgtaacgatg cttatagtgc aattaaagtg gcagtttcat tggcaaatgc ttttgaatgt
     4981 ggagtaaatg atttacctct tagtatggta ctatcatggt atgaacagaa agctgtttgt
     5041 atacttctta cattacttca cttaggtatc aaaaatatct atataggacc ttctttacca
     5101 gcattcttat ctaagaatgt attagatata ttggttgata aatttgcatt gacgcctata
     5161 tctactcctg aagaagatat gaagaagatt ttaggataaa aaaataaata tatctatact
     5221 taaatctatt tatagttaaa atatttttaa gtatagatgc aattaagata aggggttaaa
     5281 cttaaaattt gattaaaaaa atcaattttg agacaaccct ttatttattt tttgtaataa
     5341 ttgaaaatta aacctgcttt tagtatattc tttttaggaa tccttgtatt aaacttataa
     5401 tgcttagata tataatatct tatagatttt attttaagta gtaaaatttt taatggaatt
     5461 taaatattaa aaaaggaaca ttgtaatatg taacttgcat aaaatatata tactatagtg
     5521 aaattatttt aattaaaatt taaaatagaa agtgttttat gtgggggtat atgtatatga
     5581 aaaattctaa tttcaatata ttggcatttg atggtggtgg attaaaagga gcattgagta
     5641 tatccatact tgagaggata gtaaaagagt atccaaatct attaaataat ataaatatgt
     5701 ttggaggaac atctacagga agtctaattg cattaggact tgcatatggt gtaagcccga
     5761 aagagataaa agagctttac tcaatcgaaa attcaaaaca tatttttaat aaaagttata
     5821 cagaaatgct cagaccaaag tatgaaaata aaaatttaaa agaagtttta ttaaggatat
     5881 ttccagagga actagaatta aaagatttaa ataaattagt aatgatacca agtttttata
     5941 taggaaatga agaaaatgca tggaaaccag tgttttataa caatatgcca aattctttta
     6001 caaagactag taaagtagta gatgtagcaa tggcaagcag tgctgcacca gtgttttttc
     6061 caacatataa tcgccatgtt gatggaggaa taatagcaac tgacccaagt ctagcgtgta
     6121 taattcatgc cattgacagt ggatttaagc ttaaaaatac aagacttttt tcgataggaa
     6181 ctggatatgt atataatagt ataaaggcag acactactga atggggagct attgactgga
     6241 taattaataa agaacctgat ttaccaatta tatctattac attagaagga aattctcaaa
     6301 tgagtcagtt attttctcaa aaacttcttg gagataatta ctatagatta aatcctaaga
     6361 tggaaaaaga tgtagctatg gatgattgtg atgcgttaga atatttaatg tctttaggag
     6421 aaaattgtga tataaaagat gcttttgggt ggataaagaa aaagagcttt tatagaaatt
     6481 agattcaatt taaaattgta tttttacaca tgatttcatt gaatatgtaa aaataataaa
     6541 atgcagatta ttaagttact tcttagggaa taaataaaat ataatttatt taaggagtga
     6601 tgaaaatggt aaaacttgct acttttgcag gtggatgttt ttggtgtatg gtaaaacctt
     6661 ttgataagta tgatggagtt attaaagtaa tttctggata tacaggtggt catattgaaa
     6721 atcctacata tcaagaggtg tgttcgggaa caactggtca tgttgaagct attcaaataa
     6781 cttatgatga tgatataata agttatgatg agctactgaa tatattttgg aaacagatta
     6841 atcctactga tgatggtgga caatttggtg ataggggaac tcaatataag actgctatat
     6901 tttatcatga tgaagagcaa aaaaatatag ctttagagtc aaaagaacat ctagaaaata
     6961 gtaaaatatt cgatagtaaa atagtaactg atataattcc agcaactaag ttctatgaag
     7021 cagaagaaaa tcatcaggat tattataaga aaagaccatt aaactacaat atgtattata
     7081 aagggtctgg tagatataat tttgtaaaaa gtcattggga tggaaacaat ataaatagag
     7141 aagagttaaa aaagacttta actcctatac aatttgaagt cactcaaaat gatatgacag
     7201 aggtaccttt tgaaaatgag tattatgata ataacaaaga aggaatttat gttgatattg
     7261 taagtggaga agtgctattt tcttcaaaag ataaatttga ttctggatgt gggtggccaa
     7321 gtttttcaaa gccagttata gataaatcta ttattgagag aagtgatttt tctcaaggta
     7381 tgtacagagt tgaggtaaga agtgctaaag gaaattctca tttaggtcat gtatttgatg
     7441 atggtccaaa agagcttgga ggtttaagat attgtataaa ttctgcttct cttaaattta
     7501 tacctaaaga agatatggaa actttggggt atggagaata tttaaaactt tttgagtaat
     7561 tggaactatt tattataaac ttagctaaaa ggtgaaaata taagaaaata ttttcacctt
     7621 ttgttttttt gtcaataata tgataaatta ttaattgtaa tcttatttat accagtagat
     7681 taagaattta attttgaaag agagaaaaca aatgaattta aaagacttta atggaaagaa
     7741 attgaaatca gctagaactt ttagagcaaa gacgatagaa cagttgtcta aagaaactaa
     7801 aataaacaaa aaagatttaa aagcttttga ggaaaataag tatgtcccaa atatagaaaa
     7861 tacattaaaa ttatctaata tattaaattt tccaaaagag tatttttata aaaatgaaaa
     7921 tataaatgta gttgtagagg atagccactt taatcctcaa tctaaactac ctagagtaga
     7981 agaaatttct tatagagaaa aaataattat aatacataaa atatattctt ttatggagaa
     8041 ttatattaag tttccaaaag aaaacttacc aagtagaaaa gtaatgtcag atattaatat
     8101 aaatgatata gaatctcttg catataagac tagagagttt tgggatttaa atagtactcc
     8161 aatagtaaat atggtaaatt tgttagagtc taaagggatt ataatatcag gtatgaatgt
     8221 agatagaaaa tgggcaacta tttttactca aaaacagaga attagtaagg aatctaaata
     8281 tataatatct ctcggtaatg ataaaaagtc agcatcaatt agaaattata ctttggcatg
     8341 tgagcttggg tatataatat caagtgaatt aagaatacca aataagcaat tttcagaaga
     8401 tgagtatgca tgtgcatttt tattgccaaa agatattttt ttagaagatt tgattcatcc
     8461 agaagatttg gattattatg tagaactaaa gaaaaaatgg attgtaccta tatctgctat
     8521 gatttttaga gcgtataatc taggaaaaat aaattacaaa aaatataatt atcttgtgaa
     8581 tgaaatggat aaaaaaggct ggttaataga agaaccacta gacaaaatga aaggttcaag
     8641 cccaacgtat ttaaaaagag cagttgaatt attgatagaa aataaaataa tgagcgtaaa
     8701 tagtatagta tcaagtttag atgaatttgg aataaatctt tatccagaag acttagaatt
     8761 attgatggga ttaaaaaaag gactgttatc gcaagaggta aataaaaaaa gtaaagttat
     8821 aaaatttgat agtaaaaaat aggttgatat tttagcatta aaattaaaag caggtataaa
     8881 tgatttaaac attaatttaa ttaaaagtct tttcaatgag tcatttacct gcttttagtg
     8941 taaaaaagaa tcttgaatct aaagtgtttt tgaaagtgca atatattctc ttaaaacaga
     9001 gcaagattta ttaaattcgt tttgctcatc ttcagtcata ttgatttcaa taatttcttc
     9061 aataccatct tttcctataa cacatggaac acttgcaaat acatttcttt cattatattg
     9121 attttctagt aatgtagaaa ctggaagaat ttttttctca ttatgtagta tagcttttac
     9181 aatttctact aaagcagtac ctataccaaa ttctgtacaa tctttaccat ttattatatt
     9241 tatccctgtt tttttatttt tttcaactat tgatggtagg tctaaattgc tataagtatt
     9301 tgggttatcc tttatcatgt ccaagattgg tttaccacca attgaaacat gagaccaagg
     9361 aaccatttga gaatctccat gttcacccat agaataagct tgaattgatt gttgagcgat
     9421 tccagtttct tcagataaaa gccttctaag tcttgttgaa tctaaagctg tgccagttcc
     9481 aatgactttg tttttaggaa agccagattt ttcccaaata taatttgtaa ctacatcaac
     9541 aggattagat atgactataa atataccatc aaagccagaa tcaactatgg gttttactat
     9601 agattttatc attccaatag ttggtcttag taaatctaat ctagttgtat tttgtctacg
     9661 taaaggacct tctgaagagt ctgcaacact tatcactact atatcagaat ctttacagtc
     9721 agaaaaagtt cctttttcta tatgtacttt gtgtggcaga tatgaaactg catcagctaa
     9781 atctaaagct tgagcttttg atttagattc atctatatca atcataaaga gttcatcaca
     9841 aactccttga gttattaaac tgaatccaca atgagagcca acatgccctg aacctacaat
     9901 agatattttt cttggcttta ttgccatttt aaatcactcc ccctaaaata acgatacatt
     9961 tataagaata ttatatatta taaagagata atgtataagc tctataaatt tatataaatg
    10021 ttttatattt ttatgatttt cttataaagt tttataaaat attagttact gttttttatg
    10081 tattttaata ttctatcatt tcagaaaatt tatttaatat gattttatac taaaattttt
    10141 gaaaaatcaa tgttaatata ctttacacta atgaatatta atgtgataaa catatattga
    10201 aaattattaa taatagatga gattagtaaa agtgattaaa tttaatttgt aggtttaata
    10261 tatttcggta aattttttat attgtaagaa gacatggaag catatacatt tgtaaaatat
    10321 cgaaataata tacttataaa tttgttaaca ctaatatcta tgtgttaaaa tatagtataa
    10381 gattgttagc aaagaatggg attagttcta tcctagtaca attaaattaa agttatacta
    10441 agatttgtat atttaaaaac taaagtagtt tagaactaag gaaatggaga tagaattata
    10501 atggaaaaat tatactatac agaccagtat ataaaagatt ttacagcaga aatagttgaa
    10561 ataaaagaag tagaaaataa gtttcatgtt ttgttggata agacagcgtt tttccctggt
    10621 ggtggaggtc agtttgcaga cttaggaaaa atagataact atgatgtaat aagtgtatat
    10681 gaagagggga aaaaagtata tcatgtatta gataaaaaac caataaagat acatagagta
    10741 aaatgcagta ttgattggaa tagaagagaa gatggtatgc atcaacactt tgggcaacat
    10801 gtactgtcag gttgtttttt taagactttc aatgcaaata caacaggttt tcatctagga
    10861 agtgagtata gtacagtaga tatagaaggg aatttagatg aggataaaat tagagagata
    10921 gagttgcttt ctaatgaaat tattcgtcaa aatatacaag tagagacgct acttccaaca
    10981 aaaaaagaat tgaaaaagat ttggttaaga cgtgatttgc caaatactga tgaagaaata
    11041 agagtagtaa aaataggaga tttagatacg aatgcatgtt gtggagtaca tccaaaagcg
    11101 actggtgaat tgagaatgat aaaaataaag aaatgggaaa aacataaatc tagtacaaga
    11161 atagagtttt tggcaggtaa aagggcagta gatgatatat taaaaagaga tatatattta
    11221 actaaaatat gcagatactt aagttgtaat gaagaagatg ctataaatgg tattgtaaat
    11281 ctaaatagta aagtggaaga ggttctaatt aaaaaaagaa gacttgaaga agtagtgtct
    11341 acatatcaag tcaaagaaat gattgaaaaa gcaaataaga ttggcaatat ttcagtcgtt
    11401 aaaaaaattt atgaggatga agatttgaaa tatgtaaata agatagcaaa taaaattaca
    11461 gaacatgaaa ataatattgc attattagct gttaaattta atgataaaat aaatttagtt
    11521 tttgcatgtt ctaaaaattt aaaagatatt aatatgggag ttttattaaa ggatgcaata
    11581 agccttatag atggtaaagg tggtggaagt aaaatattag ctcaaggagg aggtaagaat
    11641 aatggaaatt tagaagctac acttgactat gcatttatga agatagaaaa aactctataa
    11701 atatattata taaaaaatct taatagataa aatagatatg gaggaataag tatggagcag
    11761 caaaaagatt acatagagtt atatgaaaat gaagaattaa tagagataag agaagcctgt
    11821 atgaatgatt tggatacaat agcaaaattt aattataatt tggccaaaga aacagaagga
    11881 aaagaacttg acatggatgt tttaactaaa ggtgtaaaag cattgttatt agatgagaga
    11941 aaaggaaaat atcatgtata tacagtattt gataaggttg ttgcacagat tatgtataca
    12001 tatgagtgga gtgattggag aaatggaaat ttcctttgga ttcaaagtgt atatgtagat
    12061 aaggaatata gaagaaaagg tatctttaat tatttattta attatataaa aaatatatgt
    12121 gataaagatg aaaatattgt tggaatgaga ttatatgtag aaaaagaaaa catgagtgca
    12181 aaagcaacat atgagtcttt aaacatgtac gagtgtgatt ataatatgta tgaatacgag
    12241 gtaatgcgtt agtaatataa a
//
LOCUS       DAFNNB010000117        12142 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0118, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000117 DAFNNB010000000
VERSION     DAFNNB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0118"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            52..780
                     /locus_tag="L3M60_002660"
                     /pseudo
     CDS             52..780
                     /locus_tag="L3M60_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            908..2173
                     /gene="gluD"
                     /locus_tag="L3M60_002661"
     CDS             908..2173
                     /gene="gluD"
                     /locus_tag="L3M60_002661"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-specific glutamate dehydrogenase"
                     /protein_id="HBN6227307.1"
                     /translation="MSGKDVNVFEMAQSQVKNACDKLGMEPAVYELLKEPMRVIEVSI
                     PVKMDDGSIKTFKGFRSQHNDAVGPTKGGIRFHQNVSRDEVKALSIWMTFKCSVTGIP
                     YGGGKGGIIVDPSTLSQGELERLSRGYIDGIYKLIGEKVDVPAPDVNTNGQIMSWMVD
                     EYNKLTGQSSIGVITGKPVEFGGSLGRTAATGFGVAVTAREAAAKLGIDMKKAKIAVQ
                     GIGNVGSYTVLNCEKLGGTVVAMAEWCKSEGSYAIYNENGLDGQAMLDYMKEHGNLLN
                     FPGAKRISLEEFWASDVDIVIPAALENSITKEVAESIKAKLVCEAANGPTTPEADEVF
                     AERGIVLTPDILTNAGGVTVSYFEWVQNLYGYYWSEEEVEQKEEIAMVKAFESIWKIK
                     EEYNVTMREAAYMHSIKKVAEAMKLRGWY"
     gene            2674..3813
                     /locus_tag="L3M60_002662"
     CDS             2674..3813
                     /locus_tag="L3M60_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M56"
                     /protein_id="HBN6227308.1"
                     /translation="MSNFILNGIFRTVIVSSLSILMILIFKKNVFKRFSKKFNYYIWM
                     IVVIKLFLPFTYYTFTINILRSKKDININNINLEGFNNISTVISSILLYTWITSVVIY
                     LIYTIFKYIKLKNLISDLSYEVDDEEIINLYRSILKEFDITKDIKLKYSYEVETPAFF
                     NSCVLLPPHDYKLKELDWIFRHELMHFKSKDLYLKYVILFLKIVYWFNPFVYIMDKHI
                     DLDCELYCDERVLKNRNSEEKKDYALTIINAMRKGTNTSNKFIAGLHKQSDIKKRVKN
                     MFNEKYKNGILMALTLCLLSSITFLKVNTTSVINFNVLPKGLEDAHVIGSITVTTIEF
                     TYADAPKNVREEHKRNCEELGVIPKDSDQIGVGVEYYNSLSKRSR"
     gene            4272..5294
                     /locus_tag="L3M60_002663"
     CDS             4272..5294
                     /locus_tag="L3M60_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBN6227309.1"
                     /translation="MKKKILIPVFASVMALSVSSIVNADEVNDSSQNKDDKTNTELNL
                     GEYKEVKYKVAKIKDGVAIKIREEGQVQNIAYSGDEFTVLGTQGEWVKVKVEDGEGWL
                     ATRYVDISEGVGYTNADKVNLRKDKSESSEVIEELEKGSSLLVLEENGDWLKVKDGET
                     EGYVKSSYISDKAPVIEEPQINPSVNQNVDSTNTNQQNNANTSQQNNNSNVPTANSNA
                     VQAVLNLAYSKQGCPYVWGAEGPNTFDCSGFTQYVYRNAVGKNIPRTSKAQSKYGQTV
                     SKANLQPGDLVFFTTNGSGSVSHVGIYVGGGNMIHSPSTGKTVRVTSINSAYYTARFV
                     TAKRIL"
     gene            5589..6509
                     /gene="pyrB"
                     /locus_tag="L3M60_002664"
     CDS             5589..6509
                     /gene="pyrB"
                     /locus_tag="L3M60_002664"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBN6227310.1"
                     /translation="MLESRNLIQPEDFSIEEIDEILELAQKIIDNPSKYSRICEGKLL
                     ATLFYEPSTRTRLSFESAMNRLGGRVVGFSEPNSSSASKGETLGDTMKIVSGYVDIIA
                     MRHPQSGAASEAARYTEVPFINAGDGKNQHPTQTLTDLLTIKSLKGTLENHTIGLCGD
                     LKYGRTVHSLVKAMARYKNTKFVFIAPEELKMPDYIKEAIKGHAYYETNNLDDVIGSL
                     DVLYMTRVQQERFEDKSEYERLKNYYILNKAKLEKASKDMLVMHPLPRVNEIDIDVDG
                     DDRAVYFKQAKYGMYVRMALIIKLLGISED"
     gene            6548..7240
                     /locus_tag="L3M60_002665"
     CDS             6548..7240
                     /locus_tag="L3M60_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HBN6227311.1"
                     /translation="MYKILENIYIGEDMYRMKVKGTFEGKMGQFYMLRAWDTYPVLSR
                     PISIHDIDEEGITFLYKVVGEGTQILSNLKVNDTIKLEGPYGNGYAKVDGKVALVGGG
                     IGVAPLYLVAKNIKNCDAYLGFREDVILEEEYKQVCNEVYTTVGNTFVTDIIDVEKYD
                     YILTCGPTPMMEKLVKMVEGTKTRIMVSLENHMACGVGACLVCTCKTNEGNKKTCKDG
                     PVFWGEDVIFNG"
     gene            7233..8135
                     /locus_tag="L3M60_002666"
     CDS             7233..8135
                     /locus_tag="L3M60_002666"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HBN6227312.1"
                     /translation="MGNLSVKFGSVEFKNPVIMASGTFGFGKEYNEIYDIQKLGGISS
                     KGLTLNKKPGNNGMRVHETPSGMMNSVGLENPGVQGFIDNELPFFSKLDLVRIANVGG
                     GTLEDYLLGVQMLNDKPIDIIELNISCPNVKAGGMAFGIKNEVAREVVREVRNITKLP
                     LVIKLSPNAEDIVEMAKVCEEEGADGVSLVNTFKAMAIDIKNRRPVFENVYAGLSGPA
                     IKPIALRMVHEVCKNVNIPVMGMGGITKATDAIEFIMAGATCIQVGTANFMNPRIGIE
                     IIDGINEFMDREGIKSLDEIRGII"
     gene            8220..8804
                     /locus_tag="L3M60_002667"
     CDS             8220..8804
                     /locus_tag="L3M60_002667"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBN6227313.1"
                     /translation="MSKVDVVDILKKSDALLEGHFLLSSGKHSNRYVQCAKVLRFPEY
                     AAEVLSTVVEQIKDLDIDLVVGPAMGGVIVSYELGRQLGKEAVFTERKDNTMELRRGF
                     EVKKGAKIIIAEDVVTTGKSTMETKRVLEALGGEVVGVACIADRTNHDIGMPIYSAIK
                     LDIQVYESDECPLCKEGKLPVVKPGSREFKELGM"
     gene            9021..9947
                     /locus_tag="L3M60_002668"
     CDS             9021..9947
                     /locus_tag="L3M60_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HBN6227314.1"
                     /translation="MKNQLNKSQKKVVFFGVGAVGATFAEQFLNSKYDFKILCNKERK
                     KRYLEDGFIINGKRYDFDYVTRDEYKQEADFIIIGLKYNNLKENIKELDGLVGKNTVI
                     MSLLNGVDSEEIIGERFGIEKMVYSYVTNIDAKKINNNIIHTTNGIIVFGNKDNSEDR
                     KTNIITEVFDDVNIEYVLSKDIQRDMWWKYMVNIGVNQTSAILGAPYGVFQSSEHLRE
                     LAKSAMREVVAIAQAKGISLTEDDVENSLHRILEHSKEGRTSMLQDVEAHRPTEVDMF
                     SKNICKLGKKYSIPTPINQTFFYMIKVIESRF"
     gene            complement(9998..10888)
                     /locus_tag="L3M60_002669"
     CDS             complement(9998..10888)
                     /locus_tag="L3M60_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6227315.1"
                     /translation="MFEELKTFVAVVEYKNFTKAGEYLNLSQPSVSKHIKNLENYFKV
                     VLINRSIKQKTIFITESGQILYKRAKEILNLLNITYHDVSQVSDAITGHLKIGASLTI
                     GEYILPNFLALFSQKYPDIDVEVFIKNTSIVSSHVKDYILDIGLIEGTCSSPSFIQEY
                     FFEDKMVLALPYKSHLLKDFSFDKLQDQKWIVREDGSGTRDYLDMFLSVKEIIPRSMM
                     VFGSNYAVKESVRNNLGITIVSNLVTSLPVLNNELSVIELGSSYNRHFSYIFPKDITL
                     SKAATIFIEELKIFSNLNSI"
     gene            11076..12107
                     /locus_tag="L3M60_002670"
     CDS             11076..12107
                     /locus_tag="L3M60_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HBN6227316.1"
                     /translation="MLKKKSILTLKTIREILPGLFVSVLVGYISIFISILIPKVGAAS
                     ISIFLGMFVGNLFLNQKVFQKGYKFSETDLLSYSIVLLGATLSVSTLIDLKVSGILFI
                     ILQMTITIIAALYIGKKLGFEENFRFLMASGNAVCGSSAIGATVPVVNATDKEKGIAV
                     TIVNVTGIFLMFLLPIISQFLYSHELVKTSAMIGGTLQSIGQVVASGAIVGENVKDLA
                     TIFKILRVIFLVVVVLVFGHMKNQSNKEILEEEKDEIKKKKVTIPWYVIGFFVTCALF
                     SMNIIPKEVSVLCKEISNKLEIIALAAIGLKVNVKDLVKQGKEVSLYGLFIGIVQVVS
                     AVVLIKIFI"
BASE COUNT         4789 a         1275 c         2180 g         3898 t
ORIGIN      
        1 gcaaaattct atgaagcagg aaccctgtga ctttagtcat gggaggttca ggatggaatt
       61 ttaatataca aaaacaaggt tttagctgga gtatcagcaa aacaagcaaa tactactata
      121 gaagcagtta aaagggctat tttttataat ctggagcata gacaagaagt tagagaaatg
      181 attaaaaaag aaaatataag aaaaattctt attattggaa catcaaaaaa gatggttaat
      241 caaattgtag atagattgtc aattggaagg gtttgtaagt ttataaatat agaagatata
      301 agtactaaat atgaaataga agtagcaaaa caatctagaa aagaaggaaa tcatgtaatt
      361 cctgttccag cagttgagat aaaaagtatg gcaagtggtc ttagtataaa ttctttgaag
      421 agacttttta ggaaaggaaa taataggaat atggtagttt tagaaaaaac aattataaga
      481 ccaaccttta gttatatagg aaagttttat attagtgctg atgtaattaa acagattatt
      541 gaatatgaag tatacagctt tggtagcatt gatagaataa ataaaatcaa tatagagaat
      601 tgcaataatt ttatgaatat ttttgtaagt ataaatatca atgatttaga aactataaaa
      661 aaaattgaag atatacagca gataataaaa aagtctattg agaaaatgac tttagttaat
      721 gtacaaaaaa tagatattaa cattcataaa attaaaaaaa taaacttcct ccaatgttaa
      781 aaaataaact gagaaaatga tatactaatt tgcaataaag tgtgctataa ttaaactgta
      841 aatttaggaa atggtttttc taaatggagt atacataatt ataacgtatt tataaggggg
      901 ctttcaaatg tcaggaaaag atgtaaatgt cttcgagatg gcgcaatctc aagtaaaaaa
      961 tgcatgtgat aaattaggta tggaaccagc agtttatgaa ttattaaaag aacctatgag
     1021 agttatagag gtttcaattc cagtaaaaat ggatgatggt tctataaaaa cttttaaagg
     1081 atttagatca caacataatg atgcagtagg gccaacaaaa ggtggaataa gattccatca
     1141 aaatgtttca agagacgaag taaaagcttt atctatatgg atgactttca aatgttctgt
     1201 aacaggtata ccatatggtg gaggtaaagg tggaataata gtagatccat caactttatc
     1261 tcaaggtgaa ttagaaagat taagtagagg atatatagat ggaatatata aattaatagg
     1321 tgagaaagtt gacgttcctg caccagacgt aaatactaat ggacaaataa tgtcttggat
     1381 ggttgatgag tacaataaat taactggaca aagttctata ggtgttataa ctggtaagcc
     1441 agttgaattt ggtggttctt taggaagaac agctgctact ggatttgggg ttgctgttac
     1501 tgcaagagaa gctgctgcta aattaggaat agatatgaaa aaagctaaaa tagctgttca
     1561 aggtatagga aacgttggtt cttatacagt tcttaactgt gaaaaacttg gtggtactgt
     1621 tgtagctatg gctgaatggt gcaaatcaga aggttcttat gctatataca atgaaaatgg
     1681 tttagatggt caagctatgt tagattatat gaaagaacat ggtaacctat taaacttccc
     1741 aggagctaag agaatatctt tagaagagtt ctgggcttca gatgttgata tagtaatacc
     1801 agctgcatta gaaaactcta taactaaaga agttgctgaa tctataaaag ctaaattagt
     1861 ttgtgaggct gctaatggac caactactcc agaggctgat gaagtatttg ctgaaagagg
     1921 aatcgttctt actccagata tattaactaa tgctggtgga gttacagttt cttacttcga
     1981 gtgggtacaa aacttatatg gatactactg gtcagaagaa gaagtagagc aaaaagaaga
     2041 aatagctatg gttaaagcat ttgaatctat ttggaaaatc aaagaagagt acaatgttac
     2101 aatgagagaa gctgcttaca tgcattcaat taagaaagtt gctgaagcta tgaaattaag
     2161 aggatggtac taggatttac tcttaataaa taataggata taattattta agatataaag
     2221 gggctgtaca tagtacagcc cttaaattat gtggctcagg taatgtgagc cattttttat
     2281 tttaatttat aaattatata actcaaaatt taaaacgcta taagtaagac gagtgataat
     2341 ttatggttaa tttaatttta agtaggcttt ttagaactat tctagtgaat agtttatgta
     2401 ttcttttaat attagcattt aagaaaagta tttttgttct attttaactt tgcttttata
     2461 tatttataac tgtcaaagtt atatggagtt aaattaattg atgatgttta atgcacagta
     2521 atcattacta agctataatt aactataaga taataaaaca tagttattta ttacagaaaa
     2581 tatatatttt aattaataaa tatgatatga aagtgttaag agataactaa aattaactat
     2641 aaaacactat taaataagat aggggaaaaa catatgtcta attttattct aaatgggatt
     2701 tttagaactg ttatagtgag tagtttaagt attcttatga tattaatatt caagaaaaat
     2761 gtgtttaaaa gatttagtaa aaaatttaac tattatatat ggatgatagt tgttattaaa
     2821 ttatttctac catttacata ttatactttt acaataaata tattaagaag caaaaaagac
     2881 attaatatta ataacataaa cttagaggga tttaataata tctcaactgt aattagtagt
     2941 attttactgt acacttggat tacatctgta gtaatatatt taatttatac tatattcaaa
     3001 tatataaaac ttaaaaattt aataagtgat ttatcttatg aagttgatga tgaagaaata
     3061 ataaatcttt atagaagtat attaaaagaa tttgatataa caaaagatat aaaactaaaa
     3121 tactcatatg aagttgaaac accagcattt tttaattcat gtgtgctttt gcctccacat
     3181 gattataaat tgaaagaatt agattggatt tttaggcatg agctaatgca ttttaagagc
     3241 aaagatcttt atttaaaata tgtaatttta tttttaaaaa tagtatattg gtttaatcca
     3301 tttgtatata taatggataa acatatagat ttagattgtg agctttattg tgatgaacgc
     3361 gttttaaaaa atagaaactc agaggaaaaa aaagattacg ctttgacgat tataaatgcc
     3421 atgagaaaag gaacaaatac ttcaaataaa tttatagctg gtctacataa gcaatctgat
     3481 attaaaaaga gagtaaagaa tatgtttaat gaaaaatata agaatggtat attgatggca
     3541 ttaaccttat gtctgttatc ttctattact tttttgaaag ttaatacaac tagtgttatt
     3601 aattttaatg tattacctaa aggcttggaa gatgcacatg ttattggtag tataactgtt
     3661 acgacgatag aatttacata tgcagatgct cctaaaaatg tgagagaaga acataaaaga
     3721 aattgtgaag aactaggagt gattcctaaa gactctgacc aaataggtgt tggtgtagag
     3781 tattataaca gtttatcaaa acgatcaaga tagtaatatt ttaatagatt gtaatttata
     3841 atagttataa agttttaaac agttatttat taagaaaaaa tatattttta taaatgtata
     3901 tacaaaatca caaaatgact aactttatag ttattccctc aaaaaacata tgccacatag
     3961 aaacgcataa aaaaacacat aaaacaatat ttatgcgtac tttagcctct aaaggtacaa
     4021 aattctttac ttttacagac tcgtattata taattaagta aataatgtat atttaggtga
     4081 catattggat aaactaattt atttatttta ttaatattat aatactataa aagttatata
     4141 aagtggttta caaaaaagtt acagatagag cttttattaa tgtattgtgt attagtattt
     4201 tatccttagg tacacttcac ctacaaaata gaatcataag ataattatgg gaaaatttta
     4261 ggaggtttat cgtgaagaaa aagatattaa taccagtatt tgcatcagtt atggcattat
     4321 cagtaagttc aattgtaaat gcagatgaag taaatgattc atctcaaaat aaggatgata
     4381 aaacaaatac tgaattaaat ttaggtgaat ataaagaagt aaaatataaa gttgcaaaaa
     4441 taaaagatgg agtagctata aagataagag aagaaggtca agttcaaaat atagcttatt
     4501 ctggagatga gtttactgtt ctaggaactc aaggagaatg ggttaaggtt aaagttgaag
     4561 atggcgaagg ttggttagct actagatatg tagatatatc tgaaggtgta ggatatacta
     4621 acgctgataa agtaaattta agaaaagata agagtgaaag ttcagaagta attgaagagc
     4681 tagaaaaagg aagctcttta ttggtcttgg aagaaaatgg agattggtta aaagttaaag
     4741 atggggaaac cgaaggatat gtaaaatcat cttatatatc tgataaagct cctgtaatag
     4801 aagagccaca aataaaccca agtgttaatc aaaatgtaga ttctacaaat actaatcaac
     4861 aaaacaatgc aaatactagt caacaaaaca ataattcaaa tgtgccaaca gctaactcta
     4921 atgcagttca agctgtactt aatttggcat attctaagca aggttgccca tatgtttggg
     4981 gagcagaagg accaaacact tttgactgtt ctggatttac acaatatgtg tatagaaatg
     5041 cagtaggaaa gaatatacca agaacatcta aagcacagtc taaatatgga caaactgtaa
     5101 gtaaagcaaa tttacaacca ggagacttag tattcttcac aactaatggt tctggttctg
     5161 ttagtcatgt tggaatatat gtaggtgggg gtaatatgat acattcacca tcaacaggaa
     5221 aaacagttcg tgtaactagt ataaattcag catactatac agcaagattt gttacagcaa
     5281 aaagaatatt ataatcagtt tgtaatatga caaattgata aatacataaa tatatataaa
     5341 cgctctatat tatatttaat ttaatcatgg aaataaaaaa tgattatatt ttaaatgtac
     5401 tatagagcgt ttttgtatca ctttaaatct aatcaggatt cagggtgata ttactttaaa
     5461 taggagcatt ttcatatgat gattatttgc gcaatccctt gacaggatag ggcaaaggtg
     5521 gtaagctact aacagtacaa aaatactttt aattttagtt cagagaggct aaaaaaggag
     5581 gacgaaagat gttagaatca agaaatttaa tccaaccaga agacttttca atagaagaaa
     5641 tagatgaaat attagagtta gctcaaaaga taattgacaa tccgtcaaaa tattctcgta
     5701 tatgtgaagg aaaactatta gctacattat tttatgaacc atcaacaaga actagattaa
     5761 gctttgaatc tgctatgaac agactaggag gtcgtgtagt aggcttttca gagccaaatt
     5821 catcttctgc atcaaaaggt gaaaccctag gtgatacaat gaagatagta tctggttatg
     5881 ttgatataat agctatgaga catcctcaat caggtgcagc atcagaagca gctagatata
     5941 cagaagtgcc atttataaat gcaggagatg gcaaaaatca gcatccaact caaacactta
     6001 cagacttact tactataaaa tcattaaaag gaactttaga aaaccataca ataggattat
     6061 gtggtgattt aaaatatgga agaactgtgc attctttggt taaggctatg gcaagatata
     6121 aaaatacaaa gtttgtattt atagcaccag aagagttaaa aatgccagat tatataaaag
     6181 aagctataaa gggacatgca tattatgaaa ctaataattt ggatgatgtt ataggaagct
     6241 tggatgtttt atatatgact agagttcaac aagaaagatt tgaagataaa tcagaatatg
     6301 aaagacttaa aaactattat attttaaata aagccaaatt agaaaaagca tctaaagata
     6361 tgttagtaat gcatccatta ccaagagtaa atgaaataga catagacgta gatggtgatg
     6421 atagagctgt atattttaaa caagctaaat atggaatgta tgttagaatg gctttaataa
     6481 taaaactttt aggtataagt gaagactaat agttatcata taatagtaaa ataaaggaga
     6541 ttttgggatg tataaaattc tggaaaatat atatataggc gaagatatgt atagaatgaa
     6601 agtaaaaggg acttttgaag gtaaaatggg acaattctat atgctaaggg catgggacac
     6661 ttatccagta ctttcaagac ctataagtat acatgatata gatgaagaag ggataacttt
     6721 tctttataaa gtagtaggag aaggaactca aattttgagt aacctaaaag taaatgatac
     6781 tataaaatta gaaggccctt atggtaatgg atatgctaag gttgatggaa aggtagctct
     6841 agtaggagga ggaatcggtg tagctccact atatttggtt gctaaaaata taaaaaattg
     6901 tgacgcatat ctagggttta gagaagatgt tatattagaa gaggaatata agcaagtatg
     6961 taacgaagtc tatacaacag taggaaatac ttttgtaaca gatataattg atgtggagaa
     7021 atatgattat atattaacat gtggtccaac tccaatgatg gaaaaattag ttaagatggt
     7081 agaaggtact aaaacgagaa ttatggtatc tcttgaaaat catatggctt gtggagtagg
     7141 tgcttgttta gtgtgtacat gtaaaactaa tgaaggaaat aaaaagactt gtaaagatgg
     7201 tccagtgttc tggggagagg acgtgatttt taatgggtaa tttaagtgtg aaatttggaa
     7261 gtgtagaatt taaaaatcca gtaataatgg cgtctggaac ttttggattt ggaaaagaat
     7321 ataatgaaat ttatgatata caaaaacttg gtggtataag tagcaaaggt cttactttaa
     7381 ataaaaaacc tggtaacaat ggtatgagag ttcatgagac accgtctggt atgatgaata
     7441 gtgttggtct tgaaaaccct ggagttcaag gttttataga caatgaatta ccatttttca
     7501 gtaaactaga tttagtaaga atagctaatg ttggaggtgg gacattagaa gactacttat
     7561 taggggttca aatgttaaat gataaaccta tagacattat tgaattaaat atatcttgcc
     7621 caaatgtcaa agctggtgga atggcttttg gtataaaaaa tgaagtggca agagaagttg
     7681 ttcgtgaggt aagaaacata acgaaactgc ctttagtaat aaaattatca ccaaatgcag
     7741 aagacattgt agaaatggca aaagtttgtg aagaagaagg tgctgatgga gtttcgttag
     7801 ttaatacatt taaagcaatg gcaattgata taaaaaatag aagaccagta tttgaaaatg
     7861 tatatgctgg gttatctgga ccagctataa aacctatagc tttaagaatg gtacatgaag
     7921 tgtgtaaaaa tgtaaatata cctgtaatgg gaatgggcgg aataactaaa gcaacagatg
     7981 ctatagaatt tataatggca ggagccactt gtattcaagt tggaactgca aactttatga
     8041 atcctagaat cggtattgaa ataatagatg gaattaatga atttatggat agagaaggaa
     8101 taaaaagttt agacgaaata agaggaatta tataaggtag aataaatagt attttaaaga
     8161 taaaaaatat agattaaata aacaataatt ttataacatt aacatggagg aaaaataata
     8221 tgagtaaagt agatgtagta gatatattaa agaaaagtga tgcattgttg gagggacatt
     8281 ttttattatc ttcaggaaaa cacagtaata gatatgtaca atgtgcaaaa gtattaagat
     8341 tcccagaata tgctgctgag gtattaagta cagttgttga acaaataaaa gacttagata
     8401 tagacttagt agtagggcca gctatgggtg gagtaatagt ttcttatgaa ttgggaagac
     8461 aattaggaaa agaagctgta tttactgaga gaaaagacaa tacaatggag ttaagaagag
     8521 ggtttgaagt taaaaaagga gcaaagataa taattgctga agatgttgta actactggta
     8581 aatcaactat ggagacaaag agagtattag aagccttagg tggagaagtt gtaggtgttg
     8641 catgtatagc agatagaact aatcatgata taggtatgcc tatatatagt gctataaaac
     8701 ttgatattca agtttatgaa tctgatgagt gtcctttatg taaggaagga aaattaccag
     8761 ttgttaagcc tggaagtaga gagttcaagg aattaggaat gtaataaaaa cttaattatt
     8821 tatagtagta ttaaaaaaaa gtgaattttt cagaataaaa atttggaata taaaatataa
     8881 attaaaaata aagagcagtt agccttataa atgaaggtta gctgtttttt tgttaaaaat
     8941 atatttacaa aataagagat agtagcagta caatataagt ataaaataaa aaccattgta
     9001 tgcattaggg aggggtaagt atgaaaaatc aattaaataa aagtcagaaa aaggttgtat
     9061 tttttggagt aggagctgta ggagctactt ttgcagaaca atttttaaac tctaaatacg
     9121 attttaagat tctttgtaat aaggaaagaa aaaaacgata tttagaagat ggatttataa
     9181 taaatggaaa aaggtatgat tttgattatg taactagaga tgaatataaa caagaagctg
     9241 actttataat tataggttta aaatataata atttgaaaga aaatataaaa gaattagatg
     9301 gattagttgg aaaaaataca gtcataatgt ctctgctaaa tggagttgat agcgaagaga
     9361 taataggtga aagatttgga attgaaaaaa tggtatattc atatgttacc aatatagatg
     9421 caaagaaaat caataataat attatacaca ctactaatgg gataattgta tttggtaaca
     9481 aagataacag tgaagataga aaaactaata taataactga ggtttttgat gatgtaaata
     9541 tagaatatgt tttatcaaaa gatattcaac gagatatgtg gtggaagtac atggttaata
     9601 ttggtgtaaa tcaaacttca gctatacttg gtgcacctta tggagttttt cagagttctg
     9661 aacatttaag agaattagca aaatctgcaa tgagggaagt tgttgctata gcccaagcaa
     9721 aaggtatatc tcttacagaa gatgatgtgg aaaattcatt acatagaata ctagaacatt
     9781 caaaagaagg aagaacatca atgcttcaag atgtagaagc tcatagacct acagaagtag
     9841 atatgttttc taagaatatt tgcaaacttg ggaaaaaata tagcatacct actcctataa
     9901 atcagacctt tttttacatg ataaaagtaa ttgaaagtag attttaaggg tttttgtaag
     9961 aaactttgat aattattgat tttaaatttt attaatgtta tatactattt aagttactaa
    10021 atatttttaa ctcttcaatg aaaatagttg ctgcttttga taaagttata tcttttggaa
    10081 atatatatga gaaatgtctg ttgtagctgc ttccaagctc tatgacagac aactcattgt
    10141 ttaaaacagg taagcttgtc actagattag agactattgt aattcctaaa ttatttctaa
    10201 cagattcttt gacagcataa ttacttccaa aaaccatcat actccttgga ataatttctt
    10261 taacacttaa gaacatatct aaataatctc tagttccaga cccatcttct ctgactatcc
    10321 atttttggtc ttgaagttta tcaaaactaa aatcttttaa caaatgactt ttgtatggaa
    10381 gtgctagaac catcttatct tcaaaaaaat attcttgaat aaatgaaggg gaagagcaag
    10441 taccttcaat aagaccaatg tctaaaatat aatccttaac atgagaagat acgattgacg
    10501 tattttttat aaaaacttct acatctatgt caggatactt ttgagaaaat aacgctagaa
    10561 aatttggtag aatatactct ccaatagtta aacttgcgcc aatctttaag tgaccagtta
    10621 tagcatctga aacttggctt acatcatgat aagtaatatt taaaagattt aatatttctt
    10681 tagctctttt atacaatatt tgtccacttt ctgttataaa aatggttttt tgtttaatag
    10741 acctgtttat aagcacaact ttaaaataat tttctaaatt ttttatgtgt ttacttacac
    10801 ttggctgtga taggtttaag tattccccag ctttagtaaa atttttgtat tcaacaacag
    10861 ctacaaatgt ttttaattct tcaaacaatt ttttgtctcc tttacctaat taaattttac
    10921 attttttata tactttctac agataattat actatatata ataaccatta aaaacattaa
    10981 tcattacgat attatatatt tattttactt atggttatta caattatata ctattgctat
    11041 gaagaaaact atattaatta taaggtaggt atattatgtt aaagaaaaaa agcattttga
    11101 cattaaaaac tataagggaa atattaccag ggttgtttgt atcagtatta gtaggttata
    11161 ttagtatatt tatttctatt ttaattccta aggtaggagc agcatcaatt tctatatttt
    11221 taggtatgtt tgtaggaaac ctatttttaa atcaaaaagt gtttcaaaaa ggttataaat
    11281 tttcagagac agatttgtta tcatattcaa tagtactatt aggagcaaca ctcagtgtat
    11341 ctaccttaat agacttaaaa gtttcaggaa ttttatttat aatattgcaa atgacaataa
    11401 caataatagc tgcactttat ataggtaaaa aactaggatt tgaagaaaac tttagatttc
    11461 taatggcaag tggaaatgca gtatgtggtt cttctgctat aggagcaaca gttccagtgg
    11521 ttaatgcgac agataaagaa aagggaattg cagtaactat tgtaaatgtt actggtatat
    11581 tccttatgtt tttacttcca ataatatctc aatttttata tagccatgaa cttgtaaaaa
    11641 cgtcagcaat gataggtggg actttgcaat caattggtca ggttgtagca agtggagcaa
    11701 ttgttggaga aaatgtaaaa gatttggcta ctatttttaa gatattaaga gtaatatttc
    11761 tagttgttgt tgtattagta tttggacata tgaaaaatca gtctaataag gaaatccttg
    11821 aagaagaaaa ggatgagatc aaaaagaaaa aagtaactat accatggtat gtaattggat
    11881 tctttgtaac ttgtgcactt ttttcaatga acattatacc aaaagaagta tctgtgttgt
    11941 gcaaagaaat aagtaacaaa ctagaaataa tagctttagc agcaattggt ttaaaggtta
    12001 atgtaaaaga tttagtgaaa caaggaaaag aagtttcttt atatggttta tttataggta
    12061 tagtacaagt tgtatcagct gtagttttaa taaaaatatt tatatagcac aaaaatagta
    12121 tttgtaatat caatttcatt aa
//
LOCUS       DAFNNB010000118        12127 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0119, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000118 DAFNNB010000000
VERSION     DAFNNB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12127
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0119"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(113..874)
                     /locus_tag="L3M60_002671"
     CDS             complement(113..874)
                     /locus_tag="L3M60_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6227317.1"
                     /translation="MEYTVQKLSKLAGISTRTLRYYDEIGLLKPLKINSSGYRIYGQN
                     EVNKLQQILFYRELGINLENIKNIINSPNFDSLSALKKHHNKLLVKRKQIDLLIENVT
                     KTIALKEGKYTMTDIEKFEGFKEKMIDENEKNYGTEIRENYGKDIIVASNKKFKNMSK
                     QDYEDWQNLSIEIISKLKKAFKTGDASSELAQEVARLHHKWLSYTWSTYSKEAHASLA
                     QMYVCDERFTSYYDKEQPGLTKFLRDAIVVYTRDN"
     gene            1093..1569
                     /locus_tag="L3M60_002672"
     CDS             1093..1569
                     /locus_tag="L3M60_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBN6227318.1"
                     /translation="MNLKRSNEKREEYRRMYSDKESFLSLIQNFTSVSIAKELTLKNI
                     ELETSFICEYKGKEVDVIYKVFSKNGKVSHYIVLEFQTEMDTEIVPRLKSYREQIWKS
                     FIMKKSLEEIEDKNFKLPKVIPVVLYSGPERLSRKKGILDIVESISEFSNNSSMKN"
     gene            1667..1819
                     /locus_tag="L3M60_002673"
     CDS             1667..1819
                     /locus_tag="L3M60_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate translocator"
                     /protein_id="HBN6227319.1"
                     /translation="MRNNKSKVLLGILCLATGMAVILSMLLPGWIWSALTALILIGCG
                     ALLFFC"
     gene            complement(1979..2167)
                     /locus_tag="L3M60_002674"
     CDS             complement(1979..2167)
                     /locus_tag="L3M60_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBN6227320.1"
                     /translation="MAKVCSVCGKGKVSGNQVSHSNKHNKRTWSANLRSVRAIIDGAP
                     KRVKVCTRCLRSGKIERA"
     gene            2528..2878
                     /locus_tag="L3M60_002675"
     CDS             2528..2878
                     /locus_tag="L3M60_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HBN6227321.1"
                     /translation="MSAKVTNQYGSIEIDNQVIAQVVYRAAMESYGLVGLAYKSKGIV
                     ELLKGENATKGVRVEEVEEDAIAIELYVIIQYGTNISTVANNIIDRVKYVVEKMTGVR
                     VTRIDINVQGIRVK"
     gene            2897..4540
                     /locus_tag="L3M60_002676"
     CDS             2897..4540
                     /locus_tag="L3M60_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HBN6227322.1"
                     /translation="MIQYIDGKRLREMFISGANNLQNNKDLVDKLNVFPVPDGDTGTN
                     MSLTISYALKELAKVETDNISDIGKALSKGSLMGARGNSGVILSQIIRGIAKSIEGKS
                     KLSTEDLAKAFKNGSDTAYKAVIKPIEGTILTVVRESGDFAIKTAKKEKDVVKFLSML
                     VKESNSSLERTPDLLKNLKEAGVVDSGGKGLVLIYEGMLASIKGNNIEIKNADLDTNI
                     STSMDFAKSTISTDDIKYCYCTEFILESSKVEDTKIRDIMMAYGDSLAVVGDDGVIKV
                     HVHTNDPGNVLQEALKYGQLLTIKIENMKLQHENTLLDVEEKKENDSEPLEEEKEFGF
                     IATSMGEGLANIFKDFGVDHIIEGGQTMNPSTEDFMNAIKDINAKNIFIFPNNSNIIM
                     AANQAKELSDKNIIVIPTKNTPQGFAALVTFNGELSEDENKEAMMNALSSVKSGQVTF
                     AVRDTVMNEIDVKEGNIIGIAEGNLLSAGDYVDEVTSNLIEKLVDEDTAIITLFFGED
                     VTESQANELRTSLEEKFEDVDVELYYGGQPLYYYLISVE"
     gene            4621..6678
                     /gene="recG"
                     /locus_tag="L3M60_002677"
     CDS             4621..6678
                     /gene="recG"
                     /locus_tag="L3M60_002677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBN6227323.1"
                     /translation="MDLYKDVQYVKGIGPKKADKLNKLGIFTLKDLLYYFPRQFEDRN
                     NLKKIAQLEDSEKVTIKAVISSINTFSPKEGMTLTKIDVKDETGSAKLVFFNKSYIKN
                     TFRPGDSILVFGKVKKKFNNLELTSCELEYLTNSPKNTCRFMPVYQLTYGVTNKEIMS
                     IIRTVLEDKDLIIQEYMPQRIIDKYRLCSIDFAVRNIHSPNSKESLKIALYRIVFEEL
                     LILQLGLFVFKSGRNKEDGIKFETSKDLKKIINALPFKLTKAQNRALGEIIQDMDSEK
                     IMNRLVQGDVGSGKTVVALLALANCVLNGYQGALMAPTEILAGQHYISLTESLKDFGI
                     NVGLLIGSLTKKQKDTVLEQVKNNEIDILIGTHALIEDKVEFNNIGLVITDEQHRFGV
                     MQRSKLSLKGVNPDILVMTATPIPRTLALILYGDLDISIIDELPPGRQPIETLAIEKS
                     KRDRAYNNLVRREVESGRQVYIVCPLVEESEAIEAKSAVELVEELRAEYFHDLRLGLL
                     HGKMKSSEKDEVMGLFKNKEIDILVSTTVIEVGVNVPNATLMIIENAERFGLAQLHQL
                     RGRVGRGSHKSYCVLIYDSKTDVCRQRMAIMEETNDGFKISEKDLEIRGPGEFFGTRQ
                     HGLPELKVANLFKHIKILKLAQQEARYILGEDNNLQLKENMALKKEIIDKFKDTLKEI
                     SLN"
     gene            6737..7291
                     /gene="rsmD"
                     /locus_tag="L3M60_002678"
     CDS             6737..7291
                     /gene="rsmD"
                     /locus_tag="L3M60_002678"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HBN6227324.1"
                     /translation="MRVISGKARGLKLNTPKNEDVRPTTDRVKESLFNMINSYIMESE
                     VLDLFAGTGSLGIECLSRGAKACTFVDISKESIDIVKSNIKKARVESESIILNLDFKT
                     AIDKLKLQNSKFDIIFMDPPYYKNMFIEAIEKIDNSNLLNEDGIIVVEHDTNDLFPDK
                     ICKLEKTRDKKYGNTTLTFYKMEA"
     gene            7293..7790
                     /gene="coaD"
                     /locus_tag="L3M60_002679"
     CDS             7293..7790
                     /gene="coaD"
                     /locus_tag="L3M60_002679"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBN6227325.1"
                     /translation="MENKPRKAIFAGSFDPITNGHLDIICRASKLFDELQIGVLNNPN
                     KKGLFSFDERVKLIEKSTSHLNNIKVVTFDGLLINYCQENGIGALVRGVRSGADVDYE
                     LQMAHMNRELNPDIETIILPSCTKYSFISSSLIKEVLLFDADIKNLVPKIVLEELKKK
                     TSGGN"
     gene            7794..8318
                     /locus_tag="L3M60_002680"
     CDS             7794..8318
                     /locus_tag="L3M60_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227326.1"
                     /translation="MKIDLEMIELFEQLEEIFRSASTIPFSHKCTVDKDEVLAIINDI
                     RTLIPEEVTQAVWINKERNKIISQAKQDATNIVEQAKKEAERIQQEYQDNIEELKKNS
                     EDVVKAYVESSEPVVQADQRAKDIVDRAERIANEIRIGSIEYAEDVLSSVEYNLKEIL
                     EEVKRDKAELRPKK"
     gene            8747..10039
                     /locus_tag="L3M60_002681"
     CDS             8747..10039
                     /locus_tag="L3M60_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HBN6227327.1"
                     /translation="MEGVKVGMLKKLCSLAMVAIMTTTLITGCTSGNGKDSKKDGEKE
                     KLVLWMPPVENNMKEVWDPILDKFEEKNNCEVDLQIIPWDNYSEKFATAISAGEGPDV
                     GYMYAEMYPQFIESGAVEDLTNYATKEDKEQSIYIKTSEMMGGMYGMPFQAANPGVLY
                     YNKDILEKLGEKPPKTWEDFKRICKKATQDTDGDGKIDQWGLAQGWGAKTFGNLNWNW
                     YPYLWQAGGDIFNNDLKSVKFNDKSGLEAANFLKELQAYVPEDSLSKDSNEMIESVFG
                     PGKAAFTIMLSSAATSVFDESFPDLNWGFVTGLEDKKAATFGAVDHLSLMSSAKNKEL
                     AYKLIQHMLSVESMTEFHKAIPRAPITKGEPYQGDERFKEMVENDKDVYRPLVVGPHG
                     VEIYEYLWKELQTMISGDKTPKQALDDAAKYSNDLLAQ"
     gene            10137..11054
                     /locus_tag="L3M60_002682"
     CDS             10137..11054
                     /locus_tag="L3M60_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBN6227328.1"
                     /translation="MENLVREFEENSKRKKTKLNIKPYMYILPLGIILVTFYVIPIIM
                     SIYFSFTKYNIISPATFIGLENYKKLFTDETLKVSIINTIKFTIVVVPCQTILSLILA
                     VWITGKGNSKIASFAKGAIFIPVLSSMVLIGMVWRALLNGEGSIIYQVLGAFGIESSK
                     LLGDSKTALPTLMFISMWKNIGYFMVIYISAIMNLPKHCYEVAKVDGATKLQEFIKIT
                     VPLLKPTTIMVVFLGAIWSLQVFDLVYTVTGGGPGISTMSIVMHAFNLNFKNFNSGYA
                     MTVANVLFLLIAVVSILQNKLVKRDNSDF"
     gene            11070..11879
                     /locus_tag="L3M60_002683"
     CDS             11070..11879
                     /locus_tag="L3M60_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HBN6227329.1"
                     /translation="MKTTRRILSDGLLLFIACASLVPFIYMLIISLKITYNSYSLDIS
                     FSTVTLQNYIDIFTKKGFAQYFLNTAIVSFSGVLLNLVFSTLAGYSFAKMDFKGSDKL
                     FLFMIMTLIIPSQVTMIPLYIIMKHLGWINSYLALIMPIPTAFGVFIMRQAILGVPKE
                     LLESAKIDGCSDFRILIQIVLPLIKPALITLAIFTFMGAWNEFMWPLIATTKDAMRTL
                     TVGLSTLKTFQITNYGQMMAGATITFLPPFIFYLILQSKFVEGVSLSGIKG"
     gene            11936..12109
                     /locus_tag="L3M60_002684"
     CDS             11936..12109
                     /locus_tag="L3M60_002684"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227330.1"
                     /translation="MEISYVLKDCTFKHNKYSVIETETWEDKDLDIVVAKGEKFAFQI
                     MLKATEEFNCTID"
BASE COUNT         4757 a         1365 c         2134 g         3871 t
ORIGIN      
        1 gataatcctt tttttattgt tgaaatgata aaaacatatt tgatttcata taaaaaagag
       61 tgtctcatga actaaaaagt tcattttgag acatcctctt tattatttta tattaattat
      121 ctcttgtata tactactata gcatccctca gaaacttagt tagtccaggc tgttctttat
      181 cataataaga tgtaaagcgt tcgtcacata catacatctg tgctaaagag gcatgagctt
      241 ccttagaata tgtgctccaa gtgtagctca accatttatg atgtaatctt gcaacttctt
      301 gagcaagttc actggaggca tcaccagttt taaaagcttt tttaagctta ctaattattt
      361 caatgcttag attttgccaa tcttcataat cttgtttaga catattttta aattttttat
      421 ttgaggcaac tataatatct ttaccataat tctctctaat ttcagttcca taattttttt
      481 cattttcatc aatcatcttt tctttaaaac cttcaaattt ttctatatct gtcatagtgt
      541 attttccctc cttcagagct atagttttag ttacattttc tattagtaaa tcaatttgct
      601 ttcttttgac tagtaattta ttatggtgtt tttttaacgc acttaaacta tcgaagtttg
      661 gtgaatttat aatgtttttt atattttcta agttaattcc taattctcta taaaaaagta
      721 tttgttgaag tttattaact tcattttgac catatatacg ataacctgaa gaatttattt
      781 taagtggctt aagaagacct atttcatcat aatatctaag tgtcctagta cttataccag
      841 ctagtttact taatttttga actgtatatt ccataattag tacctcctat aaactaaata
      901 tacactttta cgcaacgtaa atgtcaatat attaaaatat aaaaatttat atcttttaat
      961 aaaaatataa aaaaatatct ttactggata ttttacacac aagtgtcaat actcactagt
     1021 gaaaattaaa aaataaaatt tatagactat ttttattaaa atatagaagg tctatagttt
     1081 aggaggaact gtatgaattt aaaaagaagc aatgaaaaac gagaagagta tagaagaatg
     1141 tattcagata aagaaagttt tttaagtttg attcaaaatt ttacaagtgt ctcaatcgca
     1201 aaggaattaa ctcttaaaaa tatagaatta gaaacatctt ttatatgtga atataaagga
     1261 aaagaagttg atgtaattta taaagtgttt tctaaaaatg gtaaagtatc acattatata
     1321 gtattagagt ttcaaacaga aatggatact gaaattgtac caagattaaa atcgtataga
     1381 gaacagattt ggaagagttt tattatgaaa aaaagtcttg aagaaataga agataaaaat
     1441 tttaagcttc ctaaagttat accagtcgtt ttatatagtg gacctgaaag gttaagcaga
     1501 aaaaaaggaa ttttggatat tgtagaatct atatcggagt tcagtaataa ttctagtatg
     1561 aaaaattaaa actacataat atgtttattg gtctaaaatg gaaaactgtc gcataaactt
     1621 gaatatgaaa atatttatag atacaggttc tatgtggagg tgttccatga gaaataacaa
     1681 gtctaaggta ttgctaggaa tactttgtct ggcaacagga atggctgtaa ttctttcaat
     1741 gttgcttcct ggctggattt ggtcagcact taccgcatta atacttattg gatgtggagc
     1801 attattattt ttttgttaaa atagttttaa ctataaagtg tggggtgagt attgatgaaa
     1861 gtagtaacaa taaaattacc aaaatggatt tcaagtataa tcctaaaatt tatgagaaag
     1921 agtaaaaatg attaaattaa aaagcctact tacggaagta ggcttttaaa cgtggttact
     1981 aagctctttc aattttacca gaacgtaaac atctagtaca aacttttact ctcttaggag
     2041 ctccatctat aatcgctctt acgcttctta agttagctga ccatgttctt ttattatgtt
     2101 tatttgagtg tgatacttgg tttccagaaa ccttaccttt accacatacg ctacatactt
     2161 ttgccatcta cgcacacctc cttaaaatga taactattta aagactaaaa acatatttat
     2221 attatcataa ataacaaaaa tttgcaatag aaatatatat ttgccttaca catattgaag
     2281 aatttcttat tatcatataa aattatatat ataattttat ataaagtaaa tatatttgga
     2341 aaataattat tatttttctt tgaatatata tactatagaa gaaattttat ccaaaaactt
     2401 ttctaggttt aaattatacc atatttatag tatgatatga ttataatttt atataataaa
     2461 taagtaagaa tagtaaaaaa taaattaaga aatgttttta ataaaaataa gcttaagggg
     2521 gtcaaccatg agtgcaaagg taacgaatca atatggaagt atagaaatag ataatcaagt
     2581 aatagctcaa gtcgtatata gagctgcaat ggaaagctat ggcttagtag gtttagcata
     2641 taagtctaaa ggaatagtag agctattaaa aggtgagaac gcaactaaag gtgtaagagt
     2701 tgaggaagta gaagaagatg ccatagcaat agaactttat gttataatac aatatggtac
     2761 aaatatatct actgttgcca ataatataat agatagagta aaatatgtag ttgaaaaaat
     2821 gactggcgta agagttacta gaattgatat taatgtacaa ggaataagag ttaagtaatt
     2881 tcataggagg aaacaaatga ttcagtatat agatggtaaa aggttaaggg agatgtttat
     2941 ttcaggtgca aacaatctcc aaaacaataa agatttagtt gacaagttaa atgtattccc
     3001 tgtaccagat ggagatacag gaactaatat gtcattaaca atatcttatg ccttaaaaga
     3061 attagcaaaa gttgaaactg ataatatatc agacatcgga aaagcattat ctaaaggctc
     3121 tttaatggga gctagaggaa actcaggagt tatactttca caaataatca gaggtatagc
     3181 taaatctata gaaggaaaaa gtaaattatc aacagaagat ttagcaaaag cttttaaaaa
     3241 tggttcagac acagcatata aagctgtgat aaaaccaatt gaaggaacta tacttactgt
     3301 ggttagagaa agtggagatt ttgcaataaa aactgcaaaa aaagaaaaag atgtagtaaa
     3361 gtttttaagt atgcttgtga aggagtccaa ttcttcatta gagagaactc cagaccttct
     3421 aaagaatcta aaagaggctg gtgtagttga ctctggtgga aagggtcttg tacttatata
     3481 tgaaggaatg ttagcttcta taaaaggcaa taatatagaa ataaaaaacg ctgacctaga
     3541 tactaatatt tcaacaagta tggattttgc aaagagtact attagtacag atgatataaa
     3601 atattgttat tgtacagaat ttattctaga aagttctaag gtagaagata ctaaaataag
     3661 agacataatg atggcttatg gtgatagcct agcagtagtt ggagatgatg gagtcataaa
     3721 ggtacatgta cacactaatg acccaggaaa tgtacttcaa gaagctctaa agtatggtca
     3781 attattaact ataaaaatag aaaatatgaa gttacaacat gaaaatacat tactagatgt
     3841 agaagaaaag aaagaaaatg atagtgaacc tttagaagaa gaaaaagagt ttggatttat
     3901 agctacttct atgggtgaag gtttggcaaa tatatttaaa gattttggag ttgaccatat
     3961 aatagaaggt ggtcaaacta tgaatccaag tactgaagat tttatgaatg ctataaagga
     4021 tataaatgct aaaaatatat ttattttccc aaataacagt aatataataa tggctgctaa
     4081 tcaagcaaaa gaattgagtg ataaaaatat aatagttata cctacgaaaa acacacctca
     4141 agggtttgct gcactagtaa catttaatgg agaattaagt gaagatgaaa ataaagaagc
     4201 tatgatgaat gccttaagct cagttaaatc aggtcaagta acttttgctg ttagggatac
     4261 agtgatgaac gaaattgatg ttaaagaagg taatataatt ggaattgcag aaggaaattt
     4321 attatcagca ggagattatg tggatgaagt aacatcaaac cttattgaaa agttggttga
     4381 tgaagatact gctataataa cattattctt tggtgaagat gttacagaat ctcaagcaaa
     4441 tgaactacgt acttctttag aagaaaaatt tgaagatgta gatgtagagt tatattatgg
     4501 aggacaacct ctttattatt acttaatttc agttgaataa tgattataag aacctgtatg
     4561 accaagtagc ttttataaaa ttggttgtac aggatttttg tgtagtaggt gatatatttg
     4621 ttggatttat ataaagatgt tcaatatgtt aagggaatag gacctaaaaa agcagataag
     4681 ttaaataagc tgggtatatt tacactaaaa gacctattgt attattttcc aagacaattt
     4741 gaagatagaa ataatctaaa gaaaatagca caattagaag atagtgagaa agttacaata
     4801 aaagcagtaa tatcaagtat aaatacattt agcccaaaag aagggatgac tttaacaaaa
     4861 attgatgtaa aagatgagac aggttcggct aaattagtat tttttaataa gagttatata
     4921 aaaaatactt ttcgtccagg agattcaata ttagtttttg ggaaggttaa gaaaaagttt
     4981 aataacttag aacttacatc atgtgaatta gaatatctta ctaattcacc taagaataca
     5041 tgtagattta tgcctgtgta ccagcttaca tatggtgtta caaataaaga aattatgagt
     5101 ataattagaa cagtacttga agataaagac ttaattatac aagaatatat gccacagaga
     5161 attatagata aatatagatt atgtagtata gattttgcag ttagaaacat acattcacca
     5221 aatagtaagg aatcattaaa aatagcttta tatagaattg tatttgaaga attacttata
     5281 ctacaactag ggttgttcgt atttaaaagt ggaagaaaca aagaagatgg aataaaattt
     5341 gaaacaagta aagatttaaa aaaaataata aatgctttac catttaaact gactaaagct
     5401 caaaacagag cattaggtga aattatacaa gatatggact cagagaaaat aatgaataga
     5461 ttggttcaag gtgatgtagg aagtggtaag acagtagtag ctctcttagc acttgcaaat
     5521 tgcgttttaa atggttacca aggtgctttg atggctccta cagagatatt agcagggcaa
     5581 cattatattt cacttactga gtctctaaaa gattttggaa taaatgtagg tctattaata
     5641 ggtagtctta caaaaaaaca aaaggataca gtattggaac aagtaaagaa taatgagatt
     5701 gatatattaa taggtacaca tgcactaatt gaagacaagg ttgaatttaa taatattggt
     5761 ttagttataa cagatgaaca gcatagattt ggtgttatgc aaaggagcaa actttcattg
     5821 aaaggtgtaa atcctgatat actagtaatg acagcaacac caataccaag aactttagca
     5881 cttatacttt atggagactt agatatttct attatagatg aacttcctcc aggaagacag
     5941 ccaatagaaa cacttgctat tgaaaaaagt aagagagata gagcatataa caaccttgta
     6001 agaagagagg ttgaatcagg gagacaggtg tatattgtat gtcctctggt agaagaaagt
     6061 gaagccatag aagccaaatc agcagttgaa cttgttgagg agttaagagc ggaatatttt
     6121 cacgacttaa ggcttgggtt gcttcatggc aaaatgaagt caagtgaaaa agatgaagta
     6181 atgggacttt ttaaaaataa agaaatagat attttagttt caacaacagt aatagaagtt
     6241 ggtgtcaatg tgccaaatgc aacattaatg ataatagaaa atgcagaaag atttggactt
     6301 gcacaattac accagcttag agggagagtt ggaagaggaa gtcataaatc gtactgtgta
     6361 ctaatttatg attctaaaac agatgtatgt agacaaagaa tggctataat ggaagagacc
     6421 aatgatggtt ttaagatttc agagaaggat ttagaaataa gagggcctgg ggaatttttt
     6481 gggacaaggc aacatggtct tccagaactt aaggtagcaa acttatttaa acatataaaa
     6541 atactgaagt tagcacagca agaagctcga tatatattgg gtgaagataa taatctacaa
     6601 ttgaaagaaa acatggcact caaaaaagaa attatagata aatttaaaga tacactgaaa
     6661 gaaatttcat taaattaatt ttaaattatg ttaaaatatt actaatgtaa taatgtgctg
     6721 aagggagatt gtcatattga gagtaatttc aggaaaagcg agaggattaa aactaaatac
     6781 tccaaaaaat gaagatgtaa ggccaactac agatagagta aaagagtctc tatttaatat
     6841 gataaattca tatataatgg aaagtgaagt tctagattta tttgctggga ctggctcttt
     6901 aggaatagag tgcttatcta gaggtgccaa ggcatgtact tttgtggata tcagtaagga
     6961 aagcatagat attgttaagt ccaatataaa aaaggcaaga gttgaaagcg aaagtattat
     7021 tttaaacctt gattttaaaa ctgcaataga taaacttaaa ttacaaaata gtaaatttga
     7081 cataattttt atggaccctc cgtattataa aaatatgttt atagaagcta ttgaaaaaat
     7141 tgataattcc aacctattga acgaagatgg aataatagtt gtagaacatg atacgaatga
     7201 tttatttcca gataagatat gtaaattaga gaaaactaga gataagaaat atggaaatac
     7261 tacattaact ttttataaga tggaggcata atatggaaaa taaacctaga aaagctatat
     7321 ttgcaggaag ttttgaccca attactaatg gacatttaga tattatttgc agggcttcaa
     7381 agttatttga cgaattacaa ataggagtat taaataatcc taataaaaag ggtctattta
     7441 gctttgatga aagagtaaaa cttatagaaa aaagtacaag ccatttaaat aatataaaag
     7501 ttgtgacttt tgatggttta ttaataaatt actgtcaaga aaatggaatt ggagcactag
     7561 ttagaggcgt gagaagtggt gctgatgttg attatgaact tcagatggcg catatgaata
     7621 gagagcttaa tccagatata gagactataa ttttaccaag ttgtacaaaa tattcattta
     7681 taagttcatc tttaataaaa gaggttttgc tctttgatgc tgatataaaa aatttggttc
     7741 caaagattgt tttggaagag ttaaagaaaa aaactagtgg gggtaattga tatatgaaga
     7801 tagatttaga aatgattgaa ctatttgagc agttagagga gatatttaga agtgcttcta
     7861 caattccttt ctcacataaa tgcacagtgg ataaagacga agttttagca ataattaatg
     7921 atataagaac tttaatacca gaagaagtaa cacaggcagt atggattaat aaagaaagaa
     7981 ataaaataat aagtcaagct aagcaagatg ctacaaatat agttgaacaa gctaaaaaag
     8041 aagctgagag aatacaacaa gaatatcaag acaacataga ggaattaaag aaaaattctg
     8101 aagatgtagt aaaggcatat gtagaatcca gtgaacctgt tgttcaagca gaccaaagag
     8161 ctaaagatat agttgataga gctgagagaa tagccaatga aataagaatt gggtcaattg
     8221 aatatgcaga agatgtgctt tctagtgttg agtataattt aaaagaaata ttagaagaag
     8281 taaaaagaga taaagctgaa cttagaccta aaaaataaat tttaaataaa taaaaatgga
     8341 gctatcacac aaaaaattct aattgatgtg tgatagctct cttttaatac aacaattttt
     8401 tgttatctaa ttgtactttt catatcactt atatttcatt ttgatattat aatgcaattt
     8461 aatcttttat ttgtttaaat cagatgctgt attaatagga ttcttaattg ctatgttaac
     8521 atctacagct ttttattatt gtatattaaa aagatgagta attagttttt aaatgggtga
     8581 aaatagattg gcaaatatca attgtaaata ttagaaacgt tgtcatagaa aaaaactata
     8641 ttaaaaaaaa gtacatagaa aaaactgatt agacaataaa tacatgcttt gataatattt
     8701 taaatggaat aaaaatgtgt agaattataa tgaaaaagaa aataaaatgg agggagtaaa
     8761 agtgggtatg ttaaaaaagt tatgttcact agctatggtg gcaattatga ctacaacact
     8821 tataacaggg tgtacaagtg gaaatggaaa agattcaaaa aaggatggcg aaaaagaaaa
     8881 actagttctt tggatgccac cagtagaaaa taatatgaag gaagtttggg accctatttt
     8941 agataaattt gaggaaaaaa ataactgtga agtagattta cagataattc cttgggataa
     9001 ttattctgaa aaatttgcaa cagctataag tgctggcgaa ggtccagatg tagggtatat
     9061 gtatgcagaa atgtatccac agtttataga aagtggagca gtagaagatt tgactaatta
     9121 tgcaactaaa gaagataaag agcaatctat ttatataaaa acatctgaaa tgatgggtgg
     9181 aatgtatggt atgccattcc aagcagcaaa tccaggggta ctatactata acaaggatat
     9241 tttagagaaa ttaggagaaa aacctcctaa aacatgggaa gattttaaaa gaatttgtaa
     9301 aaaagcaaca caagatactg atggtgatgg aaagatagac caatggggat tagctcaggg
     9361 atggggagct aaaacttttg gtaacttaaa ttggaactgg tatccttatt tatggcaagc
     9421 tggtggagat atatttaata atgatttaaa atcagttaaa tttaatgata agagtggttt
     9481 agaagcagct aatttcttaa aagaattaca agcatatgtg cctgaagatt ctttatctaa
     9541 agatagtaat gaaatgatag aaagtgtatt tggcccagga aaagcagcat ttacaataat
     9601 gttatcttct gctgcaacaa gtgtatttga tgaatcattc ccagacttaa attggggatt
     9661 tgtaactgga ctagaagata agaaagcagc aacttttgga gcagtagacc atttatcttt
     9721 gatgtcatct gcgaaaaata aagaacttgc gtacaagtta atacaacata tgttaagtgt
     9781 tgaatcaatg acagaatttc ataaagcaat accaagagca ccaataacaa aaggtgaacc
     9841 atatcaaggt gatgaaagat tcaaagaaat ggtggaaaat gacaaggatg tatacagacc
     9901 attagtagta ggacctcatg gagttgaaat atatgaatat ttatggaaag aacttcaaac
     9961 tatgatttca ggagataaaa ctccaaaaca agcattagat gatgctgcaa aatattcaaa
    10021 tgatttatta gcacaataaa agtttaaagc tgtaatatgt tgtaaagtac tttacagatt
    10081 aaaattagca ttgtatttat aaatacaatg ctaatattta tattggagag gataaaatgg
    10141 aaaacttagt tagagagttt gaagaaaatt caaaaagaaa aaaaacaaaa ttaaatatta
    10201 aaccatatat gtatatattg ccactgggaa taattttagt gacgttttat gtgataccaa
    10261 taataatgtc aatatacttt agttttacaa agtataatat tattagtcca gctactttta
    10321 taggtttaga aaattataaa aaattattta cagatgaaac attaaaagta tctattataa
    10381 atactattaa atttactatt gttgtagtac cttgccaaac tatattatca ttgattttgg
    10441 cagtttggat tacaggaaag ggaaatagta aaatagcttc atttgctaaa ggtgctatat
    10501 ttataccagt attatcatct atggttttga taggtatggt atggagagct ttattgaatg
    10561 gagaaggttc tattatatat caggtattag gtgcttttgg aatagagtca tctaaacttt
    10621 taggagatag taagacagca ttacctacac ttatgtttat atctatgtgg aaaaatatag
    10681 ggtatttcat ggtgatttat atttcagcta taatgaattt accaaaacac tgctatgaag
    10741 tagctaaggt tgatggggct acaaaacttc aagaatttat aaaaataact gtacctttat
    10801 tgaaaccaac tacaataatg gttgtatttt taggggcgat atggtcacta caagtatttg
    10861 atttagttta tactgtgaca ggtggaggcc caggtatatc tactatgagt atagttatgc
    10921 atgcatttaa cttaaacttt aaaaacttta attctggata tgcaatgact gtagcaaacg
    10981 tattattttt attaatagca gtagtttcta ttttacaaaa taaacttgta aaacgagaca
    11041 attcagattt ttagaaaggt aggtataata tgaaaacaac aagaagaata ttgagtgatg
    11101 gtttactttt atttatagcg tgtgcaagtt tggtaccttt tatatacatg ttgataatat
    11161 cattgaagat aacatataac tcatatagtt tggatatatc tttctcaaca gtaacccttc
    11221 aaaactatat agatatcttt actaaaaaag ggtttgcaca atatttttta aatacagcaa
    11281 tagtatcttt ttcaggtgta ttattaaatt tagtatttag tacattagca ggatattctt
    11341 ttgcgaagat ggattttaaa ggcagtgata agttattttt atttatgata atgactttaa
    11401 ttataccttc tcaagttaca atgataccac tatatataat aatgaaacat ttaggatgga
    11461 taaatagtta tcttgcactt attatgccaa taccaactgc atttggtgta tttataatga
    11521 gacaagctat tttaggagtg ccaaaagagt tattagaatc agcaaagata gatggttgct
    11581 cggactttag aattttaata cagatagttt taccactaat caaaccagca ttaattacac
    11641 tagcaatatt tacttttatg ggagcgtgga atgagtttat gtggcctctt atagcgacaa
    11701 ctaaggatgc aatgagaaca ttaacagttg gattatctac tcttaagaca ttccagataa
    11761 ctaattatgg acagatgatg gcaggtgcta caataacatt tttacctcca tttattttct
    11821 atttgatttt gcagagtaaa tttgttgaag gtgtgtcttt atctggcata aaaggttaaa
    11881 tagtataatt taataatata aaataaactt atataaatat ggaggaattt aaactatgga
    11941 aatttcatat gtactgaaag attgcacatt taaacataat aaatactcag ttatagaaac
    12001 tgaaacttgg gaagataagg atttggatat tgtagtagct aaaggtgaaa aatttgcatt
    12061 tcaaattatg ctaaaagcaa cagaagaatt taattgtaca attgattaaa aaatagctac
    12121 gcacact
//
LOCUS       DAFNNB010000119        12111 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0120, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000119 DAFNNB010000000
VERSION     DAFNNB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12111
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0120"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            3..79
                     /locus_tag="L3M60_002685"
     tRNA            3..79
                     /locus_tag="L3M60_002685"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37..39,aa:Asp,seq:gtc)
     gene            90..164
                     /locus_tag="L3M60_002686"
     tRNA            90..164
                     /locus_tag="L3M60_002686"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122..124,aa:Gly,seq:gcc)
     gene            174..247
                     /locus_tag="L3M60_002687"
     tRNA            174..247
                     /locus_tag="L3M60_002687"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Arg,seq:tct)
     gene            1158..1997
                     /locus_tag="L3M60_002688"
     CDS             1158..1997
                     /locus_tag="L3M60_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HBN6227331.1"
                     /translation="MLDINSFLNGFFNIILRMSIYDLIDISIVAYIFYKIFMFIKDTR
                     AEQVFKGIIFLLLATQLSNTFKLHTVYWISLKALDYGVIAALIIFQPEFRAGLEHIGR
                     AKFNLFGKNVNTSEETLNRNIEEIVEALYSLSRQKIGALIIMERETRISDIINTGTII
                     DAEISRQLLINIFIPNTPLHDGAVVIRDSKVKAAACFLPLTESKDLSKDLGTRHRAGI
                     GVSEVSDCITLIVSEETGGVSIAKAGKLYRDISRERMMNILRSNLKTNTETRSFFKGG
                     IFK"
     gene            1994..3175
                     /locus_tag="L3M60_002689"
     CDS             1994..3175
                     /locus_tag="L3M60_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227332.1"
                     /translation="MINRLKNNTKIKIISFLSAIALWMYVMAVVDPEETKGFEDIPVS
                     ISNMNDLKDKNLVIYPDEKLTADVYIKGSLSKVQSVKKEDIHVHGTIEDPIQGQKEVY
                     LKANIPQGVTIEFKHDVLVVNLEKNIKEKRDIKVEVSGNSKKNINKIELSKEFVDVSG
                     PRTLVKEVKSVRANLDVGNETDDFSKKLQLIPLNSDGTKVEGVTLSSSFVTAKIELLK
                     EKIVPIRVNLNDNKDNDALKNYKLSKEQVTIKGKKEDIDKINYIKTKPIDLADLANGE
                     EKEVSLEVPEGITLEGASITIKLNDTKQLSAEFLYSAGDIELRNIPTNMDNPSVISDV
                     DIKVVVESTEDLSKLNKKDISLYIDLSQGPEEDSKYKIKYETTYQFKKINIEPNIVEV
                     E"
     gene            3479..4381
                     /gene="ptb"
                     /locus_tag="L3M60_002690"
     CDS             3479..4381
                     /gene="ptb"
                     /locus_tag="L3M60_002690"
                     /EC_number="2.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate butyryltransferase"
                     /protein_id="HBN6227333.1"
                     /translation="MRSFEEVIKFAKERGPKTISVACCQDKEVLMAVEMARKEKIANA
                     ILVGDIEKTKEIAKSIDMDIENYELIDIKDLAEASLKSVELVSQGKADMVMKGLVDTS
                     IILKAVLNKEVGLRTGNVLSHVAVFDVEGYDRLFFVTDAAMNLAPDTNTKKQIIENAC
                     TVAHSLDISEPKVAAICAKEKVNPKMKDTVEAKELEEMYERGEIKGCMVGGPFAIDNA
                     VSLEAAKHKGINHPVAGRADILLAPDIEGGNILYKALVFFSKSKNAGVIVGAKAPIIL
                     TSRADSEETKLNSIALGVLMAAKA"
     gene            4403..5482
                     /gene="buk"
                     /locus_tag="L3M60_002691"
     CDS             4403..5482
                     /gene="buk"
                     /locus_tag="L3M60_002691"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HBN6227334.1"
                     /translation="MSKIFKILTINPGSTSTKIAVFDNEDLVFEKTLRHSSEEIGKYE
                     KVSDQFEFRKQVIEEALKEGGVKTSELDAVVGRGGLLKPIKGGTYSVSAAMIEDLKVG
                     VLGEHASNLGGIIAKQIGEEVNVPSYIVDPVVVDELEDVARISGMPEISRASVVHALN
                     QKAIARRYAREINKKYEDINLIVAHMGGGVSVGAHKNGKIVDVANALDGEGPFSPERS
                     GGLPVGALVKMCFSGKYTQDEIKKKIKGNGGLVAYLNTNDAREVEERIEAGDEKAKLV
                     YEAMAYQISKEIGASAAVLKGDVKAILLTGGIAYSKMFTEMIADRVKFIADVKVYPGE
                     DEMIALAQGGLRVLTGEEEAQVYDN"
     gene            5472..6149
                     /locus_tag="L3M60_002692"
     CDS             5472..6149
                     /locus_tag="L3M60_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6227335.1"
                     /translation="MITNDKRIRIITGHYGSGKSEFAMNYVVKLRDMVSGKVAIADLD
                     VVNVYFRTREKKELMKSLGIQPIDSSINAPTLDLPAVSAEVMSPMVDHSYNSVIDLGG
                     DNVGARVIGRFSHLLKEGDYDMLFVINANREKTQTSEEVIQYIKEIEKSSKLKVTGLI
                     NNTHLIRFTTIDDVLRGQKVAKEVSEKCNIPIRYVACLENLVEQLPKDLEGEIFPIKL
                     YMREDWM"
     gene            6192..6407
                     /locus_tag="L3M60_002693"
     CDS             6192..6407
                     /locus_tag="L3M60_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6227336.1"
                     /translation="MAKGKVSFNQERCKGCGLCVEACPVKIIQLDSNVINKKGYNPAT
                     VFEMEKCIGCASCATMCPDVVITVERD"
     gene            6436..7515
                     /locus_tag="L3M60_002694"
     CDS             6436..7515
                     /locus_tag="L3M60_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HBN6227337.1"
                     /translation="MAKILMKGNEAFGKAAIEAGCKYFFGYPITPQSELPEYLSRELP
                     KIGGAFVQAESEVSAINMVYGGAGAGARVMTSSSSPGVALKQEGITYAVGAEVPCVVL
                     NVMRGGPGLGSIQPSQADYFMSTRGGGNGDYRTPVFAPATVQEAVDMIMEAFDVADYY
                     RSPVMVVADGMIGQMMEPVEFRAPEKKRELPPKDWATVGTKGKRKPNVINSLYLEPEV
                     LEDHCWHLQEKFDAMEKNEVQYEMYKTEDAEFVFAAYGTTSRVVKSAIDILREEGIKA
                     GLIRPKVLWPFPFEAFNQIPNARNILTVEMSMGQMVEDVKMAVEGRLPVYFHGRPGGM
                     TPTPAEIVEKAKKIIAGELVAGGAR"
     gene            7515..8267
                     /locus_tag="L3M60_002695"
     CDS             7515..8267
                     /locus_tag="L3M60_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HBN6227338.1"
                     /translation="MAVVFKKTEGLQDTQTHYCPGCTHGIIHRLVGEVLEELGVLGDA
                     VGVVPVGCSVLGYKYFNCDTQEAAHGRAPAAATGIKRVHPENTVFTYQGDGDLASIGT
                     AEIVHAAARGEKITTIFVNNTTYGMTGGQMAPTTLVGQRATTAQSGRNAETQGYPIRV
                     SEMLATLTGAVFVERVAVDTPAHVRQAKKAIKKAFQVQQAGLGFGIVEVLSTCPTNWG
                     LAPNDALQWLRDNMIPYYPLGNFKNVEVEEVK"
     gene            8268..8825
                     /locus_tag="L3M60_002696"
     CDS             8268..8825
                     /locus_tag="L3M60_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HBN6227339.1"
                     /translation="MSTARVICAGFGGQGVMSMGQLLTYAGMLEGKEVSWLPSYGPEM
                     RGGTANCAVTVSNEPVGSPLITDDATAAILLNIPAFEKFKDDVIPGGKIIVNSSLIKE
                     KVDRTDVDVYYIPANELAAELGNDKVANMIMLGAYLKVSDTVDIESVLEAFKKVFGPR
                     KEKFVPLNREALQKGMDATCSKATN"
     gene            8997..10343
                     /locus_tag="L3M60_002697"
     CDS             8997..10343
                     /locus_tag="L3M60_002697"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBN6227340.1"
                     /translation="MRKYFGTDGVRGVANTELTCDLAYKLGRAGGFVLAQGDHRVKVV
                     VGKDTRISGDMLEASLIAGLMSVGCDVITVGIIPTPAVAYLTRKYGADCGVVISASHN
                     PVEYNGIKFFNKNGYKLDDEIELKIEEYIDDIDKIDCMPIGENVGRKLHEHCAQRDYV
                     DYLKSIISTDFKGLKVVLDCANGASYKVAPIVFDELGASVISINSSPDGNNINYKCGS
                     THPEQLQSAVLEHNADLGLAYDGDADRLIAVNEKGQIVDGDHIMILSALNLKKNNKLA
                     QDTLVVTVMSNIGLTIAAKENGINLSTTAVGDRYVLEDMVKNGYNLGGEQSGHMIFLD
                     YNTTGDGVLSSLILANIILQEKKPLSELASIMSQYPQVLVNATIKNENKNKYMEYPEI
                     KTEIERIESILDGNGRVLIRPSGTEPLVRVMLEGKEEGQIKELATNLANLIQEKLS"
     gene            10610..>12111
                     /gene="glmS"
                     /locus_tag="L3M60_002698"
     CDS             10610..>12111
                     /gene="glmS"
                     /locus_tag="L3M60_002698"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003401499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBN6227341.1"
                     /translation="MCGIVGYLGSRKAAEVIVEGLSKLEYRGYDSAGVAVNSSNEEEL
                     NIRKFKGRLSVLAEDLEKNPIDGNLGIGHTRWATHGEPSDVNSHPHFNQAKTIAVVHN
                     GIIENYMEIKEELVSEGVKFESQTDTEVIAHLVDKYYEGNLLDAVYKTISKLRGAYAL
                     GVICKEHGNELVAVRKDSPLVVGVGEGENFIASDIPALLKYTRDVYFLENGEVVHLKD
                     ENVTVYDSNRNLVEKEVFHVTWDVEAASKGGYDYFMSKEIHEQPTGVRETLERRLDDN
                     GNIVLDSINISKEDLEKINKVYIVACGTAYNAGLLGKYAIEKFVNIPVITDIASEFRY
                     SDPFVDENSLVILVSQSGETADTLAVLRDSKAKGARILSITNVVGSSIARESDDVFYT
                     WAGPEVAVASTKAYTTQITSLYMIALDFAIKKGTITREFYDSMISKMKDIPSKIQEIL
                     DNEEYIKEVAKTVVNSDHAFYLGRGIDYSLAMEGSLKLKEISYIHAEAFAA"
BASE COUNT         4721 a         1387 c         2409 g         3594 t
ORIGIN      
        1 gcggcctggt agttcagttg gttagaatgc cagcctgtca cgctggaggt cgagggttcg
       61 agccccttcc aggtcgccaa attaaataag cgggaatagt tcagtggtag agcgcaacct
      121 tgccaaggtt gaagtcgcga gttcgaatct cgtttcccgc tccataataa tttgggtgca
      181 tagctcagct ggatagagca acgcccttct aaggcgtgtg tccggggttc gaatccctgt
      241 gcgctcatta agaaaagttt actataattt atagtaaact tttatattgt ttaaaaagac
      301 acaggtaata tacatgtatc ctgtgtcttt ttatttataa actttaaact atgtaaaaat
      361 tatagtatat tctaatagag ttacagtata ttcattaatg ttgtaaacat atgaagggta
      421 atgtataata gtctacaagg tgtttatgaa ggcaatagtt aaatatgact aattggatag
      481 aaactatata cattatattg tatactttat ggtatttata aagagattca ttttgcctaa
      541 acaataagta taagaaatta tacaaaaatt agatactata atggattgac aatttaatgt
      601 aatgtttata agttatacta aataaattat tatactgtga agtatatata actttaggta
      661 ttgtgtatta ttttcactgt agaagattct atctagttat acatgatgag ttttaatgat
      721 agtttatagt aagatattaa aatatattta tctaatctag atatgaaatg agttattact
      781 ttaatataaa aatgaaccta atgaaacaat attgatgata agtaatactt aaattttgta
      841 tctgtatata gttgataaat gtattataga atttacaata attaatatat tttagagaat
      901 aaaattgcat taaggcgcga gtactacttt gtattcgcgc cttgatagct tgaattattt
      961 aagtaaaagt aattttaata aaaaataagt atttattaag ttgatatttt taatgtgttt
     1021 agagattatt taacaatctc taaatataaa actagaatta tttacataaa agttaaatag
     1081 tttgtataga gttagattta tatagtataa tacttagtgt aaataattaa gaagttatca
     1141 aacaggagga taaaactgtg ttagatataa attctttttt aaatggattt tttaatataa
     1201 tattaagaat gagtatatat gatttaattg acatatctat agtagcatat attttttaca
     1261 aaatatttat gtttataaaa gatacaagag cagaacaagt gtttaaaggt ataatatttc
     1321 ttttattagc aactcaactt agtaacacct ttaaattgca taccgtttat tggatatctc
     1381 ttaaggcttt agattatgga gttatagctg ctcttataat ttttcagcca gagtttagag
     1441 caggacttga acatatagga agagcaaagt ttaatttatt tggaaagaat gtaaatactt
     1501 ctgaagaaac tctcaataga aatatagaag aaattgtaga agctttatat tcactttcta
     1561 gacaaaaaat aggtgctctt atcataatgg agagagaaac tagaataagt gatataataa
     1621 atacaggaac tattattgat gctgaaattt ctaggcaact gttaataaat atatttatac
     1681 caaatacacc attacacgat ggagctgttg taattagaga ttctaaggta aaagcagctg
     1741 catgtttttt gccattgaca gaaagtaagg atttaagtaa agatttgggg actagacata
     1801 gagcaggaat aggagtaagt gaggtgtctg actgtataac cttgattgta tcagaggaaa
     1861 caggtggcgt atcaattgct aaagcaggta agttatatag agatatttct agagaaagaa
     1921 tgatgaatat attacgtagt aatttaaaaa caaatacaga aacaagaagt tttttcaaag
     1981 gtggtatatt taaatgataa acagattaaa aaataatacc aaaataaaaa taatatcatt
     2041 tttaagtgca attgcattat ggatgtatgt tatggctgta gttgaccctg aagaaactaa
     2101 agggtttgaa gatatacctg tatctataag taatatgaac gatttgaagg ataaaaatct
     2161 tgttatatat ccagacgaaa aattaacagc agatgtatat ataaaaggta gtttatcaaa
     2221 ggtacaaagt gttaagaaag aagatataca tgtacatgga actattgaag acccaataca
     2281 aggacagaag gaagtgtatt taaaagcaaa tattcctcaa ggtgttacta tagaatttaa
     2341 gcatgatgtt ttagtagtga atcttgaaaa aaatataaaa gaaaaaagag atataaaggt
     2401 agaagtaagt ggaaattcaa aaaaaaatat aaataaaatt gaacttagta aggaatttgt
     2461 cgatgtgtcg ggtccaagaa ctctagttaa agaagtcaaa agtgtaagag ctaatttaga
     2521 tgttggtaat gagacagatg atttttctaa aaaattgcaa ttaataccat taaatagtga
     2581 tggaactaaa gttgaaggag taacattaag ctcatctttt gtgactgcaa aaatagaact
     2641 gttaaaggaa aaaattgtcc caataagagt aaatctaaat gataataaag ataatgatgc
     2701 actgaaaaac tataaattaa gtaaggaaca agttacaata aaagggaaaa aagaagatat
     2761 agacaaaata aattatatta agacaaaacc tattgactta gctgatttgg ctaatggtga
     2821 agaaaaagaa gttagcttag aagttccaga gggtataact cttgagggtg cctctataac
     2881 aataaaatta aatgatacaa aacaattgag tgcagagttt ttatatagtg caggagatat
     2941 tgaattaaga aatatcccaa caaatatgga taatccaagt gtaatttctg atgtagatat
     3001 aaaagttgtt gtagagtcaa cagaagattt gagtaagtta aataaaaaag atatttcatt
     3061 atatattgat ttatcacaag gtcctgagga agatagtaaa tataaaataa aatatgaaac
     3121 aacatatcaa tttaaaaaaa taaatataga acctaatatt gttgaagtgg aatgaacaaa
     3181 tatttcgaat atttagttgt tacaagatgc gacaaaaaaa cctataaaat taagtttgtt
     3241 aattttatgt ttttgttgca tcttgtttat tttagttata ttataaaata aggacaatga
     3301 gatatttgtt aaatctaaaa agcgctagat atatgaaaaa agactgataa atagatttaa
     3361 ataaatattt tgaattaaat atttcaatta tctctaggaa aacgttgctg aacgagttat
     3421 aatttaattg agatataaaa agttctattt caaaaaattc aagatgaggt ggaaatgcat
     3481 gagaagtttt gaagaagtaa ttaagtttgc aaaagaaaga ggacctaaaa ctatatcagt
     3541 agcatgttgc caagataaag aagttttaat ggcagttgaa atggctagaa aagaaaaaat
     3601 agcaaatgcc attttagtag gggatataga aaagactaaa gaaattgcaa aaagcataga
     3661 catggatatc gaaaattatg aactgataga tataaaagat ttagcagaag catctctaaa
     3721 atctgttgaa ttagtttcac aaggaaaagc cgacatggta atgaaaggct tagtagacac
     3781 atcaataata ctaaaagcag ttttaaataa agaagtaggc cttagaactg gaaatgtatt
     3841 aagtcatgta gcagtatttg atgtagaggg atatgataga ttatttttcg taactgatgc
     3901 agctatgaac ttagctcctg atacaaatac taaaaagcaa atcatagaaa atgcttgcac
     3961 agtagcacat tcattagata taagtgaacc aaaagttgct gcaatatgcg caaaagaaaa
     4021 agtaaatcca aaaatgaaag atacagttga agctaaagaa ctagaagaaa tgtatgaaag
     4081 aggagaaatc aaaggttgta tggttggtgg accttttgca attgataatg cagtatcttt
     4141 agaagcagct aaacataaag gtataaatca tcctgtagca ggaagagctg atatattatt
     4201 ggctccagat attgaaggtg gtaacatatt atataaagct ttggtattct tctcaaaatc
     4261 aaaaaatgca ggagttatag ttggggctaa agcgccaata atattaactt ctagagcaga
     4321 cagtgaagaa actaaactta actcaatagc tttaggtgtt ttaatggcag caaaggctta
     4381 attttaaggg ggaaataggc aaatgagcaa aatatttaaa atcttaacaa taaatcctgg
     4441 ttcgacatca actaaaatag ctgtatttga taacgaggat ttagtatttg aaaaaacttt
     4501 aagacattct tcagaagaaa taggaaaata tgagaaggtg tctgaccaat ttgaatttcg
     4561 taaacaagta atagaagaag ctctaaaaga aggtggagta aaaacatctg aattagatgc
     4621 tgtagtaggt agaggaggac ttcttaaacc tataaaaggt ggtacttatt cagtaagtgc
     4681 tgctatgatt gaagatttaa aagtaggagt tttaggagaa catgcctcaa atttaggtgg
     4741 aataatagca aaacaaatag gtgaagaagt aaatgttcct tcatacatag tagaccctgt
     4801 tgttgtggat gaactagaag atgttgctag aatttctggt atgcctgaaa taagtagagc
     4861 aagtgtagta cacgctttaa atcaaaaggc aatagcaaga agatatgcta gagaaataaa
     4921 caagaaatat gaagatataa atcttatagt tgcacacatg ggtggaggag tttctgttgg
     4981 agctcataaa aatggtaaaa tagtagatgt tgcaaacgca ttagatggag aaggtccttt
     5041 ctctccagaa agaagtggtg gattaccagt aggtgcatta gtaaaaatgt gttttagtgg
     5101 gaaatatact caagatgaaa ttaaaaagaa aataaaaggt aatggtggat tagttgcata
     5161 cttaaacact aatgatgcta gagaagttga agaaagaatt gaagctggtg atgaaaaagc
     5221 taaattagta tatgaagcta tggcatatca aatctctaaa gaaataggag ctagtgctgc
     5281 agttcttaag ggagatgtaa aagcaatact attaactggt ggaatcgcat attcaaaaat
     5341 gtttacagaa atgattgcag atagagttaa atttatagca gatgtaaaag tttatccagg
     5401 tgaagatgaa atgattgcat tagctcaagg tggacttaga gttttaactg gtgaagaaga
     5461 ggctcaagtt tatgataact aatgataaaa gaataagaat cataactggt cactatggaa
     5521 gtggtaaaag tgaatttgcg atgaactatg ttgttaagtt aagagatatg gtttctggca
     5581 aagttgctat agccgattta gatgtggtaa atgtatactt tagaactagg gaaaagaaag
     5641 agttaatgaa atctttaggt atacaaccaa tagatagctc aattaatgct ccaacattag
     5701 atttaccagc agtatcagca gaggtgatgt ctcctatggt ggaccattca tacaattctg
     5761 ttatagattt aggtggagat aatgtaggtg ctagggttat aggaagattt agtcatttgt
     5821 taaaagaagg cgactatgat atgctttttg taataaatgc aaatagggag aaaactcaaa
     5881 cttctgaaga agttatccaa tatataaaag aaatagaaaa atcttctaaa ttaaaggtaa
     5941 caggtctaat taataatact catcttatta gatttactac tattgatgat gttttaagag
     6001 gtcagaaagt tgcaaaagaa gtttctgaaa aatgtaatat accaataaga tatgttgcat
     6061 gtttagaaaa tcttgttgaa cagttaccaa aagatttaga aggagaaatt tttcctataa
     6121 aattatatat gagagaagac tggatgtagt ttgagctcaa atatatagaa agtttaagga
     6181 ggcttaggta aatggctaaa ggaaaagtat cttttaatca agaacgttgt aaaggttgtg
     6241 gattgtgtgt tgaagcatgt ccagtaaaaa tcatacaatt agactcaaat gtaataaaca
     6301 aaaaagggta taacccagca actgtttttg aaatggaaaa atgcatagga tgtgcaagtt
     6361 gtgcaactat gtgtccagat gtagttataa cagtagaaag agattaattt aattaatatt
     6421 taagggggaa ttttaatggc taagatactt atgaaaggta acgaagcatt tggaaaagca
     6481 gctatagaag ctggttgcaa atatttcttc ggttacccaa taactccaca aagtgaatta
     6541 ccagaatatt tatcaagaga gttacctaaa ataggtggag cgttcgttca agctgaatca
     6601 gaagtttctg cgataaacat ggtttatggt ggagcaggtg caggagcaag agttatgact
     6661 tcttcatctt caccaggagt tgctttaaag caagaaggaa taacatatgc agtaggagca
     6721 gaagttcctt gtgttgtact taatgtaatg agaggtggtc caggacttgg aagtatacaa
     6781 ccttcacaag ctgactactt catgtctaca agaggtggag gaaatggaga ctacagaact
     6841 ccagtattcg caccagctac agttcaagaa gctgttgata tgataatgga agcttttgat
     6901 gttgcagatt attacagatc accagttatg gtagttgcag atggtatgat aggtcaaatg
     6961 atggagccag ttgaatttag agctcctgag aagaaaagag aattgcctcc taaagattgg
     7021 gcaactgttg gaactaaagg aaaaagaaaa cctaatgtaa taaactctct atacttagag
     7081 ccagaagtac tagaagacca ttgttggcat ttacaagaaa aatttgatgc aatggaaaaa
     7141 aatgaagtac aatatgaaat gtacaaaaca gaagatgctg aatttgtatt tgcagcttat
     7201 ggaactactt caagagtagt aaaaagtgca attgatatat taagagaaga aggaataaaa
     7261 gcaggtctta taagacctaa agtattatgg ccattcccat tcgaagcatt caaccaaatt
     7321 cctaatgcta gaaacatatt gactgttgaa atgagtatgg gacaaatggt ggaagatgtt
     7381 aaaatggcag tagaaggtag attaccagtt tacttccatg gaagaccagg aggaatgact
     7441 ccaacaccag cagaaatagt tgaaaaagct aaaaaaatta ttgccggaga attggttgca
     7501 ggaggtgcta gataatggct gttgtattta agaaaactga aggattacaa gatactcaaa
     7561 ctcactactg tccaggatgt acacatggta taatccatag attagttggt gaagtgttag
     7621 aagaactagg agtattaggt gatgctgtag gtgttgttcc agttggatgt tcagttttag
     7681 gatataaata ttttaactgt gacactcaag aagctgctca tggtagagca ccagctgctg
     7741 ctacaggaat aaaaagagtt catcctgaaa atacagtatt cacttatcaa ggtgatggag
     7801 acttagcttc aatcggtact gctgaaatag ttcatgcagc tgctagagga gaaaaaataa
     7861 ctactatatt tgtaaacaat acaacttatg gtatgactgg tggacaaatg gctccaacta
     7921 cattagttgg acaaagagct actactgctc aatcaggaag aaatgctgaa actcaaggat
     7981 atccaataag agtaagtgaa atgttagcta cattaacagg agctgtattt gtagaaagag
     8041 tagcagttga tactccagct catgttagac aagctaaaaa agctataaag aaagcattcc
     8101 aagttcaaca agcaggatta ggatttggaa tagtagaagt attatctact tgtcctacaa
     8161 actggggact agctccaaat gatgctttac aatggttaag agacaatatg ataccatact
     8221 atccattagg aaatttcaaa aatgttgaag ttgaggaggt gaagtaaatg tctacagcta
     8281 gagtaatatg tgcaggcttc ggaggccaag gtgttatgtc tatgggacaa ttacttactt
     8341 atgcagggat gctagaagga aaagaagttt cttggttacc atcttatgga ccagaaatgc
     8401 gtggaggtac agcaaactgt gcagtaactg tatctaatga accagttggt tctccactta
     8461 taacagatga tgcaactgca gctatattat taaatatacc agcatttgaa aaattcaaag
     8521 atgatgttat tcctggtgga aaaataatag taaatagttc tttaataaaa gaaaaagttg
     8581 atagaacaga tgtagatgtt tattatatac cagcaaatga attagcagct gaattaggaa
     8641 atgataaagt tgctaatatg ataatgttag gagcttactt aaaagtatct gatactgtag
     8701 atatagaatc tgtactagaa gcattcaaaa aagttttcgg acctagaaaa gaaaaattcg
     8761 ttccgttaaa cagagaagct ttacaaaaag gtatggatgc tacatgctct aaagcaacta
     8821 actaataaag aagaaaagat gtcaaaaaaa agacaataga attatttcta ttgtcttttt
     8881 tgctgataag tattaaaata tttttatgac tgaaattaag cctataatga tattttatga
     8941 actataaaaa tataatacaa attacaagct tttcaattat gaaaggagaa tctttagtga
     9001 gaaagtattt tggaacagat ggagttagag gagtagcaaa tacagaacta acatgtgatt
     9061 tagcatataa acttggtaga gctggtgggt ttgtcttagc tcaaggagac cacagagtaa
     9121 aagtagtagt aggtaaggat actagaatat caggtgatat gttggaagca tctttaatag
     9181 caggacttat gtctgttgga tgtgatgtta ttactgttgg aataatacca acacctgcag
     9241 tggcatattt aactagaaaa tatggtgctg attgtggtgt ggtaatatct gcatctcaca
     9301 atcctgttga atataatgga atcaaattct ttaataagaa tggatataaa ttagatgatg
     9361 aaattgaatt gaaaatagaa gaatatatag atgatataga caaaattgat tgtatgccaa
     9421 ttggtgaaaa tgtaggaaga aaattacatg agcattgtgc acaaagagat tatgtagact
     9481 atttaaaatc aataataagc actgacttta aggggttaaa agtagtttta gactgtgcta
     9541 atggtgcgtc atataaagta gcaccaatag tgtttgatga attaggagca agtgtaattt
     9601 caataaatag ttcaccagat ggaaataata taaactataa atgtggctct acgcatccag
     9661 aacaacttca aagtgctgtt ttggaacata atgctgactt aggtcttgca tatgatggag
     9721 atgctgatag attaattgca gtaaatgaaa agggtcaaat tgttgatgga gaccatataa
     9781 tgatattgag tgccttaaat ttaaagaaaa ataataaatt agcacaagat actttggtag
     9841 ttacagttat gagtaatata ggattaacaa tagctgctaa ggaaaatgga ataaatttat
     9901 ctactacagc agttggagat agatatgttt tagaagatat ggttaaaaat ggatataatc
     9961 ttggtggaga acaatctgga catatgatat ttttagatta caacactact ggagatggag
    10021 tgcttagttc tctaatactt gcaaatataa tcttgcaaga aaagaaacct ttatcagaac
    10081 ttgcatcaat aatgagtcaa tatccacaag tattagtaaa tgcaacaata aaaaatgaaa
    10141 ataagaacaa atatatggaa tatccagaaa taaaaactga gatagaaaga atagaatcta
    10201 ttttagatgg aaatggtaga gttttgataa gaccatcagg aactgagcca ttagttagag
    10261 ttatgttaga gggtaaagag gaaggtcaaa taaaagaatt agcaacaaat ttagctaatc
    10321 ttatacaaga aaaattatcg taatttaaag ggtattctca gatataaata ctgaggacac
    10381 ctttctaagc gccagaactt agttatagac ataattattg agtaatatag gaactaagtt
    10441 gacgaggtca gagttaatcg aattgtcggc ggatgctctg cggtgtgttt accatcgaaa
    10501 gacttcttta aataatagga gtaatcctat ggacaaaggg aagccaaagt aaacttatat
    10561 gacctaactc ttaaaatata taaataatat ttttaggagg acacaaataa tgtgtggaat
    10621 agttggatat ttaggaagta gaaaggcagc agaagttata gtagaaggac tttctaagct
    10681 agagtacaga ggttatgact cagcaggagt agcagttaat agttctaatg aagaagaact
    10741 taatattaga aagtttaaag gtagactttc tgtacttgca gaagatttag aaaagaaccc
    10801 tattgatggg aatttaggga tagggcatac aagatgggca actcatggag aaccatctga
    10861 tgtaaactca catcctcatt ttaatcaagc caaaacaata gcagttgttc ataatgggat
    10921 aatagaaaac tatatggaaa taaaagaaga attagtttct gaaggtgtta aatttgaatc
    10981 tcaaacagat acagaggtaa tagcacattt agtagataaa tattatgagg gtaatttatt
    11041 agatgctgta tacaaaacta tatcaaaatt aagaggagct tatgcattag gtgttatatg
    11101 taaagaacat ggaaatgaat tagttgcagt aagaaaagat agtcctcttg tagttggtgt
    11161 tggtgaaggt gaaaacttta tagcatcaga catacctgca ttattaaaat atactagaga
    11221 tgtttatttc ttagaaaatg gagaagtagt tcatctaaaa gacgaaaatg taacagttta
    11281 tgactctaat agaaatcttg tggaaaaaga agtatttcat gtaacttggg atgtagaagc
    11341 tgcgtctaaa ggtggatatg actacttcat gtcaaaagaa atccatgagc aaccaacagg
    11401 agttagagaa actttagaaa gaagacttga tgataatgga aatatagtct tagacagtat
    11461 aaatatttct aaagaagatt tagaaaagat aaataaagtt tatatagtgg cttgtggaac
    11521 agcatataat gctggattac ttggtaagta tgctatagaa aaatttgtaa atataccagt
    11581 aataactgat atagcctcag aatttagata tagcgaccca tttgtagatg aaaattcatt
    11641 agtaatatta gttagccaat ctggtgagac agcagatact ctagcagtat taagagattc
    11701 aaaggctaaa ggagcaagaa tactttcaat aactaacgtt gtaggttcat ctatagcaag
    11761 agaatcagat gatgtattct atacttgggc aggacctgaa gtagcagttg catcaactaa
    11821 ggcatatact actcaaataa cttctttata catgatagct ttagactttg ctatcaaaaa
    11881 aggaacaata actagagaat tttatgatag tatgatttct aagatgaaag acataccatc
    11941 aaaaatacaa gaaatactag acaatgaaga atatataaaa gaagttgcta aaacagttgt
    12001 taattctgac cacgcattct atttaggaag aggtatagat tatagcttag caatggaagg
    12061 ttcattgaaa ttaaaagaga tttcatatat acatgcagaa gcttttgcag c
//
LOCUS       DAFNNB010000120        12085 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0121, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000120 DAFNNB010000000
VERSION     DAFNNB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 12085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12085
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0121"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..394)
                     /locus_tag="L3M60_002699"
     CDS             complement(<1..394)
                     /locus_tag="L3M60_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBN6227342.1"
                     /translation="MSKIRVVHYINNFFAGIGGEEKADIPPEKRAGVVGPGIAFQSQF
                     NDDAEIISTVICGDTYFGENIESATQELLKMIKEENPDLFIAGPAFNAGRYGVACGTI
                     CKAVEEELNIPVVTGMYKENPGVDMFKLD"
     gene            complement(471..1757)
                     /locus_tag="L3M60_002700"
     CDS             complement(471..1757)
                     /locus_tag="L3M60_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006807454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBN6227343.1"
                     /translation="MKLELGNIFIKNVEFGEKTEVKDGVLYINSEEIEKIVLEDERIV
                     SVNVELARPGESIRIAPVKDVIEPRVKIGDESKIFPGIINKVKTVGSGRTHVLLGACV
                     VTCGNIVGFQEGVIDMSGPTAKYTPFSKTNNICIVIKAKDGIDTHDYEEAARIAGLKI
                     GAYVGEAGREVEPDEVIVYETKPLLKQVKEYPDLPTVAYVHMLQSQGLLHDTYYYGVD
                     AKQIVPTFMYPTEIMDGAIISGNCVAPCDKVTTFHHLNNPVIHDLYKRHGKDLNFIGV
                     ILTNENVFLVDKERSSDMVAKLIEFLGVDGVLVTEEGYGNPDTDLMMNCRKCSEVGAN
                     VVLITDEFPGKDGKSQSIADATKEADAVVSCGQGNLVVHFPAMEKIIGTLDYVEMMIG
                     GYKGCLNEDGSMDAELQIIIASTIANGYNHLTARYY"
     gene            complement(2036..3358)
                     /locus_tag="L3M60_002701"
     CDS             complement(2036..3358)
                     /locus_tag="L3M60_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HBN6227344.1"
                     /translation="MKILIVDDELEYGVVMKKILQKKGYLVDVTLSGEEAINIIKKDK
                     NYDLVLSDVMMKNMDGVQLLDRIKAINKDIEVILVTGYGSIENAVDAMKKGALSYFIK
                     SNPIENLLEEVEKVKTSKTSVSLQKNNLEFTLESKNRDFNDVIKIAKKAACKDVNILI
                     LGESGVGKDILARYIHSISPRKNEIFVPVNCCSFSENLLESELFGHEKGSFTGAVDSR
                     KGRFELSNKGTLFLDEIGDIPLNVQVKLLRTLEDKSIERIGSNKSIKVDFRLICAMNK
                     EPKVEISNGNIREDFFYRISTITITIPPLRKRREDLATLIEFFLNKYQIEHDKKIHSI
                     DKEVKDFLLNYNYPGNIRELKNIINRLVVLSEEGNLSKDNLNLISNNVYIDDKISIKP
                     LREIRKEFECEYIEKVLSLCGNNISNTAKKLEISRRQLTNKIAEYNIK"
     gene            complement(3358..5352)
                     /locus_tag="L3M60_002702"
     CDS             complement(3358..5352)
                     /locus_tag="L3M60_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBN6227345.1"
                     /translation="MNKKRIVIIGIIYSFLVIFSLTNMYVNMEYNLNVFEYIKKSLPF
                     TEEEKKWLEKHKNLIYSSDQSSPPLRYKGKEDGQYKGIVVDLINSLSIQIGRDFYFKP
                     NNWWKESFINSIDDSIKFFDLIPSKERASKFIFTDPIYTLSANILKDKKSEDINSYMD
                     LKGKTVAIPEGDYSINFLKQKVQDINILLTPDIKTGVNHLISGKVDAVVGDEPVLRYY
                     INNYGISNKYSVLSNPIYTKKAVLAVPKQYEELVSILNKGIFKLQKNGVYKDLKKKWY
                     STYNEVDDILYERGIVPSIYLFIGIILISIYVFYSYTYLLKIEIKRKTEEVMENKKTL
                     EATFNSITDIIMLVDENNNIVESNKVLYDVMGEMSYKIADLIDMIKGVIENTFSENTN
                     KTSEIEVHNKILKINTFPVEYKKNNTEYIVVLIKDITNDKIVEAKLLRENKMISIGQL
                     ASGVAHEIRNPLGIIRNNCYLLKDNVTMEEVNDCVKSIESNVDRASNIITNLLNFARI
                     SDDNLEHINIRNFIENIVKLQYKILQLKNVEIKIDCEHNLVCYINGESLKHVFINLIS
                     NSIDAIHQDGKIIIYCYEKNHCLFIDFKDNGEGIKEDALRDIFNPFYTTKPIGEGTGL
                     GLYITYNEIKKNNGDISVESKLGVGTCFHIKIPLNREVTI"
     gene            complement(5439..6686)
                     /locus_tag="L3M60_002703"
     CDS             complement(5439..6686)
                     /locus_tag="L3M60_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HBN6227346.1"
                     /translation="MQITTFGAIFGLLIAIILIIKKFQAVYSLMLGAFIGGLVGGANI
                     TQTVDFMATGAMNISPSILRALASGVLAGSLIKTGAVDKISEQIVKIFGEKRALLSIA
                     ISTMVLAGVGVNLDVSIITVAPIGLYIGRKLNYSKLSILFAMLGGGKAGNIISPNPNT
                     IAVSGNFSVNLSSVMIANIIPAIVGVIITVILASILINKGNKVQSYEILEKREDLPSL
                     FKSLCGPIIAIFLLFLGNVFPIVIDPMIALPIGGIVTLIVTGNLNNSREYLAFGLSKM
                     QGVCILLLGTGTIAGIIQMSELQQSTIAALQFLNMPQFLLAPVSGILMSLATASSTAG
                     ATIASSTFHDAIINGGLSPISGASIINSGSSVFEQLPHGSLFHTSAGSINMDIGERFK
                     LIPYEALIGIVMTIISTSIQLVL"
     gene            6904..7449
                     /locus_tag="L3M60_002704"
     CDS             6904..7449
                     /locus_tag="L3M60_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227347.1"
                     /translation="MNDLNLGEKIAEVRKKQNLSIRDLAKLADVTPSLLSQIERGLAN
                     PSVNSLKSIASSLNVPLFTFFVSEVDKKNLIVRHNNRKKVILPGSKNVVYEILIPDSS
                     GNLEFAIMNLAPNTSSCVDRITHNGDEIAYVLEGEVKLFMDDDEFTLFKGDSVKVPLG
                     TKHKWQNDSNSESKVIFAVIL"
     gene            7463..8149
                     /locus_tag="L3M60_002705"
     CDS             7463..8149
                     /locus_tag="L3M60_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HBN6227348.1"
                     /translation="MKTYKFIFFDADDTLFDFRKSESHAFKKLLSEFNLELNFENYIK
                     SYRNISDKLWLDLEKNIITLNELKLLRFELFANKINLDVDSETLSKMYLNFLGECTFL
                     IPGAIDILPYLKEKYTIVIITNGIYEVQKKRLENSRIKGYIDGIVVSEELKISKPNPE
                     IFKYALKKFNCHDKSSALMVGDSLTSDILGGINSGIDTCWLNSNNNINYTNHIPTYEI
                     NTLLELNKLL"
     gene            complement(8273..8893)
                     /locus_tag="L3M60_002706"
     CDS             complement(8273..8893)
                     /locus_tag="L3M60_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227349.1"
                     /translation="MNYNSNKDKFILADINLADIFIDKNIVLKFLGYGNRKAPDTISE
                     IVEREIRNINDILDIKIYISEVNIDKTPKECKKAFAVMYTIGDKIDIKMNDYMKNCNM
                     MAGLALDKIGIVCLDCINEKIKMYLKEKYDSLKISHEIYPGDKEFEVERQKDIYNYIK
                     NKYSSVEIEINDYHQLSPIKSVAMLILMGDEENLESRCSKCPRKCF"
     gene            complement(8883..9905)
                     /locus_tag="L3M60_002707"
     CDS             complement(8883..9905)
                     /locus_tag="L3M60_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBN6227350.1"
                     /translation="MFSDMTLLQYINSEDRGFFLPDMGTNGLFLINKKAYEVYDSVDL
                     QLELAKTMDKYFESDFIYSFCDGITFCETLGLETLRPDYDFPSVLTHTITDREKLRKL
                     DVPDPYTSGRMPLNIESLSLISKSIKKPLYESIQGPFTLAGQLAGATQLLRCIITDKK
                     FVEELLEFTTELVRRYAVAANKAGAKYISIAEPTSVTLSKDRFDEYIVKNLNKIYDEL
                     DCWKGMHICGDTRELLDNMLDCNIDAVSLDQILDYEEIAPMIPRDIVLIGNLDPIKLL
                     GRSKPDKIRRETLKLLKKMRGYDNFLCDFGCNCLNTTPVENLQAAIKAGRISYKELDR
                     IDINEL"
     gene            complement(9906..10544)
                     /locus_tag="L3M60_002708"
     CDS             complement(9906..10544)
                     /locus_tag="L3M60_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423639.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBN6227351.1"
                     /translation="MDILDDIAECILNMGVDNIENLVKIAIDKNIDVEDIYEYGLNKG
                     MIGALDKFENKEYYLSEVIVCTDALNKGINILKKTGKVKKKSKGVILMSVVEGDTHEI
                     GKNIVKVMVEATGYKVIDLGVNRKSEDIIEEAIKNNVDIIGLSSMMTTTMENMKSVID
                     ELNRIKVDKRPKVIIGGGPVSMEFAEEIGADGYSSNAPKAVKLINKLIGGEV"
     gene            complement(10671..11822)
                     /locus_tag="L3M60_002709"
     CDS             complement(10671..11822)
                     /locus_tag="L3M60_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004452985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227352.1"
                     /translation="MELRPRSAKLEEFDKVIELINYVFRISRNHKPTMMEEFPLLLSK
                     NNIENMIIISEDDKVVSDVNYLIQDVSIQGNRLKVAAIGGVCTHPDYEKRGYSSKILD
                     KVEEKMFYDGVDIVIISGTRSLYSRRNCSLVKSFYKYTIKPEDVKIPYEIVEFDETNF
                     EKDDDLDKMIELYNQNSTRFIRTRDEFQKLLHAATIAWGTIAYNKVFIKENNNIIGYL
                     IIRTIKKEDSTVGEVAEIGLNSVNVENILKYVANKFGLEYLNYKVHVKNLKDQLKCNG
                     IKSLDYQQGTMKIINFTKLCDSLRSYFSQYVDSELLKYMEFKQVENKYIIKYKEEELV
                     IESLDKLNKLFFEKNEEQYNEFKHLKNIYKFATKAFPVDFPWTANLNYQ"
BASE COUNT         3931 a         2090 c         1197 g         4867 t
ORIGIN      
        1 aatctaactt aaacatatct acacctggat tttctttata cattcctgta actacaggaa
       61 tatttagctc ttcttcaact gccttacaaa tagtcccaca agctactcca tatctacctg
      121 cattaaatgc tggacctgca ataaataaat caggattttc ttcttttatc atttttaaaa
      181 gttcttgagt tgcactctca atattttcac caaaatacgt atctccacaa attacagttg
      241 agattatctc tgcatcatca ttaaattgac tctgaaatgc tattccaggt cctactacac
      301 cagccctttt ttctggcgga atatctgctt tttcctctcc acctatacca gcaaagaaat
      361 tgtttatata atgaaccact ctgattttac tcatctttta accctccaaa tcactttata
      421 gttattacat caatgattac accaacagaa ctttatattc aaatgcttat ttaataatat
      481 cttgctgtca aatgattata gccatttgct atagttgatg ctattataat ttgtaactct
      541 gcatccatgc ttccatcttc atttaagcat cctttatacc cacctatcat catttcaaca
      601 tagtctaaag ttcctataat tttttccatt gctgggaaat gaaccactag atttccttga
      661 ccacaagaaa caactgcatc tgcttcctta gtagcatctg ctatagattg actcttgcca
      721 tcttttccag gaaattcatc tgttatcaaa actacatttg ctccaacttc actacatttt
      781 ctacaattca tcattaggtc agtatctgga tttccataac cttcttctgt cactagaact
      841 ccatcaactc ctaaaaattc tattaattta gctaccatat cagagcttct ctctttatca
      901 actaaaaata cattctcatt agtcaaaata actcctataa agtttaaatc ttttccatgt
      961 cttttatata agtcatgtat aactggatta tttaaatgat ggaatgtcgt aaccttatca
     1021 catggtgcta cacagtttcc agatattata gctccatcca ttatttcagt tggatacata
     1081 aatgtaggaa ctatctgttt agcatccacc ccgtagtaat aagtatcatg taaaagtcct
     1141 tgtgattgta acatatgaac atatgctact gttggcaggt ctggatattc ttttacttgt
     1201 tttaataatg gctttgtctc atatacaatg acttcatctg gctcaacttc tcttcctgct
     1261 tcgcctacgt atgcacctat ttttaatcca gcaattctag ctgcttcttc ataatcatga
     1321 gtatctatac catcctttgc ttttataaca atacatatat tatttgtttt tgaaaaaggt
     1381 gtatatttag cagtaggacc actcatatca attacgcctt cttgaaatcc aactatattt
     1441 ccacaagtta caacacaagc tcctaaaagt acatgggttc ttccacttcc tacagtcttc
     1501 actttattta taattccagg aaatattttg ctttcatctc caattttaac tctaggttct
     1561 attacatctt taactggagc tattcttatt gattcgccag gtcttgcaag ttcaacattt
     1621 acacttacaa tcctttcatc ttctaatact attttttcta tttcttcgct attgatatat
     1681 agtaccccat cttttacttc tgtcttttct ccaaattcaa cattttttat aaatatattc
     1741 cctagctcta gcttcatatg aaaatccccc ttaacatatt taatcgcaat taaaagtatt
     1801 tattacgttt attaatgtat gaagcatttt ctatgccaac tatttaaaat atatattcta
     1861 tgtattaatg tcatagaaat ttgcattttt aatttacatc agtattgaat ttaccaataa
     1921 tcattgtaaa tattaatttt taaaattata ataaaaaaag ataaacagat tttttctgtt
     1981 tatcttttag tacagtttga gaaatttttt tctcacttaa tgagaagttt ttttctcact
     2041 taatattata ctctgctatt ttattggtta actgtcttct actaatctcc aattttttag
     2101 ctgtatttga aatattattt ccacataaag ataaaacttt ttcaatatac tcacattcaa
     2161 actcttttct tatttctctt aaaggtttaa tacttatttt atcatctata tacacattat
     2221 ttgatattaa atttaaatta tcttttgata gattaccttc ttcgcttaaa actacaagtc
     2281 tatttattat attttttagt tctcttatat ttcctggata attgtaattt aataaaaagt
     2341 cttttacttc cttatcaatt gagtgtatct ttttatcatg ttctatttga tacttattta
     2401 agaaaaattc tataagagta gctaagtctt ctcttctctt tcttagtggt ggtatagtta
     2461 ttgttatcgt acttattcta taaaaaaaat cttctcttat atttccattt gatatttcca
     2521 cttttggttc cttattcata gcacaaatta atctaaagtc aactttaata gatttattgc
     2581 ttcctatcct ctctatagat ttgtcctcaa gtgtccttaa aagttttacc tgaacattta
     2641 aaggtatatc tcctatttca tccaaaaata aagttccttt attcgaaagt tcaaatctac
     2701 cttttctact atctactgct cctgtaaaag aacctttttc atgaccaaat aactcagact
     2761 ccaatagatt ttctgaaaaa gagcaacaat ttacaggtac aaatatctca ttctttcttg
     2821 gacttatgct atgtatatat ctagctaata tatcttttcc tacaccagat tcacccaaga
     2881 ttaatatatt aacatcttta caagcagctt tttttgctat ttttatgaca tcattaaaat
     2941 ctctattttt agattccaaa gtaaactcca aattattttt ttgtaaagaa acagatgttt
     3001 tacttgtctt tactttttca acttcttcta ataaattttc aataggatta ctttttataa
     3061 aataagataa agctcctttt ttcatagcat caactgcatt ttcaatactt ccataccctg
     3121 taactaatat aacctctata tctttattaa tggctttaat tctatctaat agctgtactc
     3181 catccatatt tttcatcata acatcagata aaaccaaatc ataattttta tctttcttaa
     3241 ttatatttat agcttcttca ccacttagtg ttacatctac aagatagccc ttcttttgta
     3301 gaattttttt cattactact ccatactcta gctcatcatc aactattagt attttcatta
     3361 tatagtcacc tctctattta atggaatttt tatatgaaaa caagttccaa cccccaattt
     3421 actttcaaca cttatatcgc cattattctt tttaatctca ttataagtaa tgtataaacc
     3481 taaaccagtt ccctcaccaa ttggttttgt agtatagaaa ggattaaata tatcccttag
     3541 agcatcttct tttattcctt ccccattatc tttaaagtct ataaataaac aatgattttt
     3601 ttcatagcaa tatataataa tttttccatc ttgatgtatt gcatctattg aattagaaat
     3661 taaatttata aatacatgtt ttaatgattc tccatttata tagcaaacta aattatgttc
     3721 acaatcaatt tttatttcaa cattctttaa ctgtaatatt ttatattgaa gtttcactat
     3781 attttcaata aaatttctta tatttatatg ttctaaatta tcatctgata ttctcgcaaa
     3841 atttaacaaa ttagttatta tgttacttgc cctatctaca ttactttcta ttgatttaac
     3901 acaatcattt acttcttcca ttgtaacgtt atcttttaag agatagcaat tatttcttat
     3961 tatacctaaa ggatttctta tctcatgtgc taccccagaa gctaattgtc ctatagatat
     4021 cattttgttt tctctaagta atttcgcctc tactatttta tcattagtta tatctttaat
     4081 aagaactact atgtactcag tattattttt tttatactct acaggaaaag tatttatctt
     4141 taaaatctta ttgtgtactt ctatttcact agttttatta gtgttttcag agaaggtatt
     4201 ttcaataaca ccttttatca tgtcaatcaa atctgctatt ttataactca tttcacccat
     4261 aacatcatat agtactttgt tcgattctac tatattgtta ttttcatcta caagcattat
     4321 tatatctgtg atactattaa aagttgcctc taaagttttt ttattttcca ttacttcttc
     4381 tgtttttctt tttatttcta tctttaacaa atatgtatag ctataaaaaa cataaattga
     4441 tattaaaatt atacctataa aaagataaat actaggtaca attcctcgct catacaatat
     4501 atcatcaact tcattatatg tagaatacca ttttttcttt aaatctttat acactccatt
     4561 tttctgaagt ttaaaaatgc ctttatttaa aatactgacc agctcttcat attgtttagg
     4621 tacagcaaga actgcctttt tagtatatat aggattactt agaacactgt atttatttga
     4681 aattccataa ttatttatat aatatcgcaa tactggttca tcaccaacaa ctgcgtctac
     4741 ttttcccgat attaaatggt tgactccagt ctttatatct ggagtcaata aaatgtttat
     4801 atcttgaact ttttgcttta aaaaatttat agaataatca ccttctggaa ttgctactgt
     4861 ttttcccttt aaatccatgt aactatttat atcttcagat tttttatctt ttaatatatt
     4921 tgcactcaaa gtgtatattg ggtcagtaaa tataaattta cttgcccttt cctttgatgg
     4981 aatcaagtca aaaaacttta tactatcatc tattgaattt atgaaagatt ctttccacca
     5041 attattgggt ttaaaataaa aatctctacc aatttgaatt gacagagaat ttattaaatc
     5101 aacaacaatt cccttatatt gtccatcttc tttaccctta tatctcaaag gaggtgaact
     5161 ttggtcagaa ctataaatca aattcttgtg tttttctaac cacttttttt cttcttctgt
     5221 aaacggaagt gatttcttaa tatattcaaa tacatttaga ttatactcca tatttacata
     5281 catatttgta agtgaaaata tcactaaaaa ggaataaata atccctataa taacaatcct
     5341 ttttttattc actttaaaac tccaatcttc ttttttacta taataaattc aatatttaat
     5401 aacaaaatcc tacttttttc attgaatatt tatatttatt ataaaactaa ttgaatagat
     5461 gttgaaatta tagtcattac tattcctatc agtgcttcat atggaattaa tttaaatctt
     5521 tcacctatat ccatatttat actacctgca ctagtatgaa atagtgagcc atgtggtaat
     5581 tgttcaaata cactagaccc agaatttatt attgatgcac ctgaaattgg tgataatcca
     5641 ccatttatta tagcatcatg aaaagtagat gatgctatag tagcaccagc tgttgatgat
     5701 gctgttgcta acgacataag tatacccgaa actggtgcca ataaaaattg tggcatattt
     5761 aaaaattgta gagcagctat tgtgctctgt tgtaattcag acatctgtat aattcctgca
     5821 atagtccctg ttccaagtaa taatatacag accccttgca tcttagataa tccaaaagct
     5881 aaatattctc ttgaattgtt caaatttcct gtgacaatta gagttactat tcctcctatt
     5941 ggaagagcta tcatggggtc tataactata ggaaatacat ttcctaaaaa caatagaaat
     6001 atagctatga ttggaccaca taatgattta aataaacttg gtaagtcctc tcttttctct
     6061 aagatttcat atgattgtac tttattccct ttatttatta atatacttgc taaaataact
     6121 gtaataataa ctccaacaat tgctggaatt atatttgcaa tcataacact agagagattg
     6181 acagaaaaat tacctgatac tgctatagta tttgggtttg gtgatattat attacctgct
     6241 ttacctcctc ccaacatggc aaatagtatt gacaatttag aataatttaa ttttctacct
     6301 atatataatc ctataggtgc tacagttatt atagatacat ccaaatttac tcctactcca
     6361 gcaagtacca tagttgaaat ggcaatcgaa agtaaagccc tcttttcacc aaatattttt
     6421 acaatctgtt ctgatatttt atctactgcc cctgttttta ttaaacttcc agctaataca
     6481 cctgatgcca aagctctaag aattgatggt gatatattca tagctccagt tgccataaaa
     6541 tctacagttt gtgttatgtt tgctcctcca actaatcctc caataaatgc tcctaacatt
     6601 aaactatata cagcttgaaa tttttttata attaaaataa ttgctattaa taaaccaaat
     6661 atagctccaa aagtcgttat ttgcataata tcccctccta tcataaatta attatctttt
     6721 atttaaatat attttaagta ttctgtatcc actttgtcaa ttatatttta atttgtttaa
     6781 ttagattgaa catagttttt tcaaaacctc attctatatg ttataataat ttcatactat
     6841 ataatctttt tacttgatta ttttaagtta atatttttta aattaaaaag agaggatatt
     6901 tatatgaatg atttaaattt aggtgaaaaa atagctgaag ttcgaaaaaa acaaaactta
     6961 agtattcgag atttagcaaa actagctgat gttacaccat cattattaag ccaaatagaa
     7021 cgaggtcttg ctaatccatc tgtaaattca ttaaaatcca tagcctcttc tctaaacgtt
     7081 cctttattta cattttttgt atctgaggta gataaaaaaa acttgatagt aagacataat
     7141 aatagaaaaa aagttatttt acctggaagc aaaaacgttg tatatgagat tttaattcca
     7201 gattctagtg gcaatttaga gtttgcaatt atgaatttag ctccaaatac ttcttcttgt
     7261 gttgatagaa ttactcataa tggagatgaa attgcatatg ttttggaggg agaggttaaa
     7321 cttttcatgg atgatgatga atttacttta ttcaaaggag atagtgtaaa agttccttta
     7381 ggaacaaagc ataaatggca aaatgactca aatagtgaaa gtaaagtaat ttttgcagtt
     7441 atcttgtaaa ggagtaaaaa ccatgaagac atataaattt atattttttg atgcagatga
     7501 tactttattt gattttagga aaagtgaatc acatgctttt aaaaaacttc tatctgagtt
     7561 taatttagaa cttaattttg agaattatat aaaatcatat agaaatataa gtgataagct
     7621 ttggcttgac cttgaaaaaa atattataac tttgaatgaa cttaaattac ttagatttga
     7681 actttttgca aataaaataa atttggatgt tgattctgaa acccttagca aaatgtactt
     7741 aaatttttta ggtgaatgta catttttaat tccaggtgca atcgatatat tgccatatct
     7801 taaagaaaaa tatactatag ttataataac aaatggaatt tatgaggttc aaaagaaaag
     7861 acttgaaaat tcaagaataa aaggatatat tgatggaata gttgtatctg aagaattaaa
     7921 aatatcaaaa ccaaatcctg aaatatttaa atacgctctc aaaaagttta attgccatga
     7981 taaatcttct gctttaatgg ttggtgatag cttaacctca gacatcttag gtggaataaa
     8041 ttcaggaata gatacttgtt ggttaaattc caacaacaat ataaattata ctaatcatat
     8101 accaacttat gaaataaata cacttcttga attaaataaa ctactatagc ttgtaaatct
     8161 gttcagatta aacagaatat tattttgcaa taattttttt agtttatata caaaataact
     8221 aaaaaaatta ttgcaattca tacacagaac aaatctcagt cttgaaaact atctaaaaac
     8281 attttctagg acacttacta catcttgatt ctaaattttc ttcatccccc attaaaataa
     8341 gcattgcaac actttttatg ggcgatagtt gatgatagtc attgatttct atttctacac
     8401 tactatattt attttttata tagttataaa tatccttttg tctctctact tcaaattctt
     8461 tatctcctgg atatatttca tgcgatattt tgagactatc atacttttct tttaaataca
     8521 tttttatttt ctcattaatg caatctaaac atactatacc tattttatcc aatgctagtc
     8581 ctgccatcat gttgcaattt ttcatatagt cgttcatctt gatgtcaatt ttatccccta
     8641 ttgtatacat tacagcaaat gcctttttac attctttagg tgttttatca atattaactt
     8701 cacttatata tatcttaata tctaaaatat catttatatt tcttatttct ctttcaacga
     8761 tttcactaat tgtatcagga gcttttctat ttccatatcc taaaaacttt agtacaatat
     8821 tcttatctat aaaaatatcc gctaaattta tatctgctaa aataaattta tctttattac
     8881 tattataatt cattaatatc aatcctatct aattctttat aactaattct tcctgccttt
     8941 atagctgctt gtaaattttc tactggagtg gtatttaaac agttacatcc aaaatcacat
     9001 aaaaaattat catatcctct catcttcttt aacaatttta gtgtctctct tctaatttta
     9061 tctggtttag aacgtccaag aagttttatt gggtctaaat taccaatcaa cactatatct
     9121 ctaggaatca ttggagcaat ttcttcatag tctaaaattt ggtctaaact tactgcatct
     9181 atattgcaat ctaacatatt atctaataat tctctggtat ctccacatat atgcatacct
     9241 ttccaacaat caagttcatc atagatttta tttaaatttt ttactatata ttcatcaaat
     9301 ctatctttac ttagagttac tgatgttggc tctgctattg aaatatactt tgctcctgct
     9361 ttatttgcag ccacagcata tctccttacc aattcagttg taaattccaa caattcttct
     9421 acaaattttt tatcagttat tatacatctt aaaagttgag tagctccagc taattgacct
     9481 gcaagtgtaa atggaccttg tatcgattca tatagtggct ttttaatact ttttgatatc
     9541 agtgataagc tttctatatt gagtggcata cgtccactcg tataagggtc tggaacatct
     9601 aattttctta atttctctct gtcagttata gtatgtgtca atacacttgg aaaatcataa
     9661 tctggtctta atgtttctaa acccagagtt tcacaaaatg taatcccatc acagaatgag
     9721 tatataaaat ctgattcaaa atatttgtcc atagtctttg ctaattctaa ttgcaaatct
     9781 acactatcat atacctcata tgccttttta tttattaaga acaatccatt tgttcccata
     9841 tcaggtagaa aaaaacctct gtcttcacta tttatatact gaagtaaagt catatctgaa
     9901 aacatttaaa cttcacctcc tataagttta tttattaatt tgactgcttt tggagcattt
     9961 gaactatatc catctgctcc tatttcttct gcaaactcca tacttacagg acctccacca
    10021 attattactt ttggtctttt atctacctta atcctattaa gttcatctat aacagatttc
    10081 atattctcca tagtagttgt catcattgaa gataagccta ttatatccac attgtttttt
    10141 atagcttctt ctattatgtc ttcacttttt ctatttacac ctaagtcaat aactttatat
    10201 ccagtggctt ctaccattac ttttacaata ttttttccta tctcatgagt atctccttct
    10261 actacagaca ttagaattac tccctttgat tttttcttta cttttccagt ttttttcaaa
    10321 atattaatgc ctttatttaa tgcatctgtg catactataa cttctgaaag atagtattct
    10381 ttattttcaa atttatctaa agctcctatc atacctttgt ttaaaccata ttcataaata
    10441 tcttccacat caatattttt atctatagca atttttacta aattctctat attatctaca
    10501 cccatattta atatacattc tgctatatca tcaagtatgt ccatataata ctcatccttt
    10561 ctaattactt acaaaattac cttttcaata tccaataatt tcttttattt aacaaggact
    10621 agctctaggt atcaatcaat tttatttaat atcatctaaa agtacaacaa ctattgataa
    10681 tttaaatttg cagtccatgg gaaatctaca ggaaatgcct ttgtagcaaa tttatatata
    10741 ttttttagat gtttaaattc attgtactgt tcttcatttt tttcaaaaaa cagtttatta
    10801 agcttatcca aactttctat tacaagttct tcttctttat atttaataat atatttattt
    10861 tcaacttgtt taaattccat gtattttaac aattcagaat ctacatattg actaaaataa
    10921 cttcttaagc tatcacacaa cttagtaaaa tttattattt tcatagttcc ttgttgatag
    10981 tccaaagatt ttatcccatt acatttcaac tggtctttaa gatttttcac atgtacttta
    11041 taatttaaat attctaatcc gaatttattt gcaacatatt tcaatatatt ttcaacattt
    11101 acagagttta accctatttc agcaacttct cctacagtag agtcttcttt ttttattgtt
    11161 cttattatca aatatcctat tatattatta ttttccttta taaatacctt attgtaagca
    11221 atcgttcccc aggcaatagt tgctgcatgc aatagttttt gaaactcatc tctcgttctt
    11281 ataaatctag tactattttg attatatagt tcaatcattt tatctaaatc atcatctttt
    11341 tcaaaattag tttcatcaaa ttcaactatt tcatatggta tttttacatc ttcaggtttt
    11401 attgtatatt tatagaaact ctttaccaaa ctgcaatttc ttctactgta tagacttctt
    11461 gttcctgata taattacaat atctacacca tcataaaaca ttttttcttc taccttatcc
    11521 aatatcttag atgaatagcc tctcttttca taatctggat gagtacatac tccacctata
    11581 gctgcaacct tcaatctatt tccttgaata gatacatctt gaattagata gtttacatca
    11641 gaaaccactt tatcatcttc tgatattata atcatatttt ctatattatt tttacttagt
    11701 aaaagaggaa attcttccat catagttggt ttatgatttc ttgatatcct aaatacatag
    11761 tttattaatt ctattacttt atcaaattct tctaattttg cacttcttgg tcttaattcc
    11821 attcatattc ctccaaatat tatttaatat acttaaaact ttattatcta taataaaaca
    11881 tttattacta cagttctaat tatatctttt ttctttacaa tttttaattg tttttttcag
    11941 caaattttta taaaatataa acctaatttc tagttaaact tataaaataa cccattatgt
    12001 ataatatatt aaaatatttt ctatatattt atatgactaa gttctataac taaattgaaa
    12061 taatcacttt ttcaagaatg ttata
//
LOCUS       DAFNNB010000121        11922 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0122, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000121 DAFNNB010000000
VERSION     DAFNNB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0122"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            239..913
                     /locus_tag="L3M60_002710"
     CDS             239..913
                     /locus_tag="L3M60_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HBN6227353.1"
                     /translation="MSLFIISQCITSFAFTPENNKFKVKPLPYAYDALEPYIDKETMK
                     LHHDKHYQAYVDKLNVALEKYPELYNYSLCELLQNLDSLPKDIATAVRNNAGGAYNHK
                     FFFDIMTPEKTIPSESLKEAIDRDFGSFEKFKQEFQKAALDVFGSGWAWLVATKDGKL
                     SIMTTPNQDSPVSKNLTPIIGLDVWEHAYYLKYQNRRNEYIDNWFNAVNWNGALENYK
                     NLKSQD"
     gene            1026..1562
                     /locus_tag="L3M60_002711"
     CDS             1026..1562
                     /locus_tag="L3M60_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBN6227354.1"
                     /translation="MIKETHERGGYILALLILPFINNLYLVKYDIAYKIVLIIVYIYF
                     AYLGSLFPDIDMRGSYISKKFTLIYKLFGSRFRHRGFTHSLIALLLIYSFFKSLTIFT
                     NNNIVFSCLSSGFIIGYLSHLCLDLITKEGIELFYPITINISLLPIKTNSKTEKLISK
                     LLNFIVIFLIGYQFYILL"
     gene            complement(1729..2349)
                     /locus_tag="L3M60_002712"
     CDS             complement(1729..2349)
                     /locus_tag="L3M60_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBN6227355.1"
                     /translation="MQYIQELLDLTQNYWVLATIIGLLSAFIESFIPALPLVAIVTAN
                     AAIQGLFVGCLLSWIGSGLGTASLFLLISRFNDSKLFNKLRNSKTEKAISWMDKQGFK
                     LLFIAYACPFMPGCLVTIASAFGKKDVKDFVPAMLAGKFVMFIVISYIASDIEGFITS
                     PLKIASFILLVFLSWKIGNRVNKNLENHNYDFHHKKHQSDKDDKMI"
     gene            complement(2560..5091)
                     /locus_tag="L3M60_002713"
     CDS             complement(2560..5091)
                     /locus_tag="L3M60_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HBN6227356.1"
                     /translation="MSLKSLKIKENLYWVGSLDPDLRVFDIIMYTPYGTTYNSYVLKG
                     TEKTVLFETVKDKHFDNYIERLNDLNIDFEKIDYIVVSHTEPDHAGSVEKLLDLAKNA
                     KVVASETAIKYLKEIVNKDFEYVAVTDGDTLSIGDKTLEFFSVPMLHWPDTIYTYIKE
                     DKTLVTCDSFGSHYSNDKIVNTLDENEEKDYLDALRYYYDCIMGPFKPSMVTAIEKIK
                     DLDIDTVCPGHGPVLTENPRKIIDLYYNWSVNEQIKLEKEVTICYVSAHGYTKIMAEA
                     IKAYIEKNSDYKVNLFDVIEHKQEDILAKISVSQGVLFGTPTILGDALKPIWDILVSL
                     NPVLHGGKVASVFGSYGWSGEGIENAMERISQLRMKAVKPFAVNFKPSNEEIDKLNSY
                     TGKFLDKLNATFGSKKKTKKFKCVICNEVFEGDSAPSVCPVCGAKEDQFIEIEEDEVT
                     FRKDTDEYFVIVGNGAAGFYAADAIRKRNKTCKITMISNEDELTYYRPALSDGINEEL
                     GSDFYMEDKAWYDKNNIVVILGTNVDKLDEVNKTIIVNDGAIKFDKLVIATGSRNFIP
                     PIKGHDLENVFTLRNIKDLYSVKEALEKSKKVVVIGGGLLGLEAAWEFRLKGLEVVVI
                     EAMDSILSKQLDKEGSKILEQCVRDTGIDVRLGVAVDGIEGDGKAQKVIFKDGDSVDC
                     DMVVFSIGVRANTQMAHDTSVKIDRGIVVDKTLQTTVKDIYACGDVAQVENTSLAIWP
                     SSVEMGKIAGANASGDNLTFESEVYPVSLDAMNVKVFSIGNIQNFDKEISSKDEGQRI
                     YKKLFMKDDSLVGAILINDLSCTVKLIRLISEKGDFEDIMKADIL"
     gene            complement(5197..5814)
                     /locus_tag="L3M60_002714"
     CDS             complement(5197..5814)
                     /locus_tag="L3M60_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HBN6227357.1"
                     /translation="MCKKTYLLVVSILIAGLLTACSGSPTADSGQAQDNTKKEANASN
                     NALKDEKNNENLMEQNFKVPYTDAINTFKDKYKDADVVDLSLERDLNKFVYTVEGVDD
                     NNEYKMKIDANTKDVLEDKTENLDSEDLNGVARKEKLDLNDIITPQQAMEIALKEQNG
                     MVKEWSLDKDLDVTFYKIRIDKDKNEYDIKIDSKKGTVLKVEKED"
     gene            complement(6173..7057)
                     /locus_tag="L3M60_002715"
     CDS             complement(6173..7057)
                     /locus_tag="L3M60_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBN6227358.1"
                     /translation="MDTTKIDDISKLDLKNLDINKMDMGGIMESLLEWATTSGVKLII
                     GILILSIGFKIIKKFVNHVMLLLSKRDIDLTLRRFLKSLLLSVLKVAVIIIVLEYWGM
                     SLSSFAAVIASAGVAIGLALQGSLSNFAGGFIILLIRPFKVGDYIEAAGHGGTVEQIG
                     LFYTQLVTPDNKQILIPNGSVSNDSLINYSAKNTRRVDLTFSVGYEDDILHVRRVLKD
                     IVNNHKLIINEPEPFIGVVEHGDNAIKFAARVWCNTEDYWTIYLDLLEEVKVRFDEEG
                     ITIPYPKMDLTVKELNKI"
     gene            complement(7249..8499)
                     /locus_tag="L3M60_002716"
     CDS             complement(7249..8499)
                     /locus_tag="L3M60_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6227359.1"
                     /translation="MKSYTKAKWSVWGIITFSFVLVLFLRMSTAVVSDNLENELGFNS
                     IQISNIASFCLYAYAFMQIPAGILIDKYGARKISSLGIIMASLGSILFGLIQSIELAY
                     ISRVIVGAGTSVILLCILKVQGRWFNKSEFASATAKFSFVGNLGGVLATFPLVFLSEL
                     VGWRNSFLLIGIIGVVIGCFMYIIVRDTPKEYGFNVDTELYEESEKVNIVDGIKSVIK
                     NKSTWYNSMIMLSFVGLTSAFISLWGVRYIMDVYCVSKSFSAFIVSFFTYGFIFGSLI
                     MDFVFAKIRSSKFNIIKYGAIIDLFIWIVIVVVYQVKPPIIVLPISFFIMGCIVMSHL
                     QVFNDAKYKNKEIYSGLATSVINTFEFIGSGIINLIIAISLQVNSYNVVDGYKKSFVV
                     FIVLSIITIVSSHIGVKNDDFKAL"
     gene            complement(8745..9509)
                     /locus_tag="L3M60_002717"
     CDS             complement(8745..9509)
                     /locus_tag="L3M60_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227360.1"
                     /translation="MLAKLLKYELKASGRVFIPLYIAILIVAVFNGIFMNTNILQIQG
                     IAILVLTSLFMALAVLTIVVTIQRFRKNLLGDEGYLMFTLPVSTSSLILSKCITALIY
                     AVVSFIVAIFTFGVLMLFGTSGILLPEILDLFNTSFKWISENFLDILVLLLVMLTSYS
                     LFILLLYTSISMGQLPKFNKHRNIVAFASFIAINIVISIVGDAVGSILPKENTNMIYH
                     FYQSSHVMFAILGDIVVIVALFFATKFILDKKLNLE"
     gene            complement(9503..10228)
                     /locus_tag="L3M60_002718"
     CDS             complement(9503..10228)
                     /locus_tag="L3M60_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227361.1"
                     /translation="MDKNINNISQDGIVEFKGVNKSYGIKTVLKNIDLNIPKGKIVGL
                     LGPNGSGKSTMIKLMNGLLQPDNGEIMINGMKPSIETKKIVSYLPERTYLNDWMKVSD
                     LLKFFYDFYSDFDVRRANEMIKSLDIDVNEKLKTMSKGTKEKVQLILVMSRNASIYIL
                     DEPIGGVDPAARSYILKTILKNYSEDSTLLIATHLISEIENICDEVIFISKGEIVLQG
                     DVEAIREEKGKSIDALFREEFKC"
     gene            complement(10230..10598)
                     /locus_tag="L3M60_002719"
     CDS             complement(10230..10598)
                     /locus_tag="L3M60_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6227362.1"
                     /translation="MEWELDNNKPIYIQLVEHLKLKIISGEIKIGSKLETVRALAEDA
                     EVNPNTMQKALTELERQGLVYSQRTKGRFVTDDKEKIKAMKEEIANVEINTLKVTLEK
                     LGYDRDEILKLITENLKGEL"
     gene            complement(10774..>11922)
                     /locus_tag="L3M60_002720"
     CDS             complement(10774..>11922)
                     /locus_tag="L3M60_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6227363.1"
                     /translation="DLFGYEVGNEILRNLTKIIKSNIFEEEMFARISADNFIIIMKYI
                     EEEDITNRLKIIFEELDLFNNNQEEKYKLVLSCGIYFILPEDRDINSIIDRANIPHKM
                     AKGGHKSSYAFYDNKIHDQEIKEKEMENTMFSSLENKEFIIYLQPKIELNTGEIQGSE
                     ALVRWKRPDKGLIPPNEFIPFFEKNGFVINLDLYVLEEVCIYLRKWIDAGINPVTISV
                     NVSRIHLYCNNFIETYKNIIDKYNIPAKYIELELTESIIFDNFDILIDIMNNLKKIGF
                     LISMDDFGSGYSSLNMLKEIPMDILKLDQKFIMETYNSKRSKIIVTKVVEMAKELGMK
                     VISEGVETEEQFELLKEVKCDMAQGYLFGKPMPIEEFEHLIVSNLIRG"
BASE COUNT         4087 a         2045 c         1153 g         4637 t
ORIGIN      
        1 atcaccttta taataaattg aaatattgcc gtccatataa ttaaaattcc tttctgcatt
       61 ttagtcttat tctatatttc atagttttgg ctttggcttt aaaacttatt caatttaaaa
      121 tgaaattgct attaaattct tataaattgt ctgttaatat ttcatatttt ttttatttct
      181 agaaatacta tattaggagg tgatttttat gaagaaaaaa atattaatac cagttattat
      241 gtctttattt ataatttcac agtgcataac ttcatttgct tttacacctg aaaacaacaa
      301 atttaaagtt aaaccattac cttatgcata tgatgcactt gaaccttata tagataaaga
      361 aacaatgaaa ttgcatcatg ataaacacta tcaagcttat gttgataaat taaatgttgc
      421 tcttgagaaa tatcctgagc tttataatta ttctttatgt gaattattgc aaaatttaga
      481 ttctttacct aaagatattg ccacagctgt aagaaacaat gcaggtggag cttataatca
      541 taaattcttt tttgatataa tgacaccaga aaaaaccata ccttctgaat ctttaaaaga
      601 agctattgat agagactttg gttcttttga aaaatttaag caagagttcc aaaaagctgc
      661 tttagatgtc tttggttctg gttgggcttg gcttgtggct actaaagatg ggaaattatc
      721 tattatgact actccaaatc aagatagccc tgtaagtaaa aacctaactc ctataatagg
      781 gcttgacgtt tgggagcatg cttactattt aaaatatcaa aatagaagaa atgaatacat
      841 tgacaactgg tttaatgcag taaattggaa tggtgcttta gaaaattata aaaatttaaa
      901 atctcaagat taattaaata ttaaaatcct tttgaaaaaa acttatgttt gctatataat
      961 ataataaaag atagtctaaa tttaccaata taatgtttat agtgacgatt caggaggatt
     1021 tttatatgat aaaagaaaca catgaaaggg gaggttatat attagcactt ttgatactcc
     1081 cctttataaa caatctgtac ctagttaaat acgatattgc atataaaata gtattaatta
     1141 tagtttatat ttattttgca tatttaggtt ctttgtttcc agatatagat atgagaggtt
     1201 cttatataag taaaaaattt acgttaatat acaaattatt tggaagcagg tttagacata
     1261 gaggatttac tcacagtcta attgccttat tacttattta tagttttttt aaatctctaa
     1321 caatcttcac taacaataat atagttttct catgtttgtc cagtggattt ataataggat
     1381 atctatctca cttgtgtctt gaccttatta ctaaagaagg tattgaattg ttttatccca
     1441 ttactataaa catttcttta ctacctataa aaactaactc aaaaacagaa aaattaatat
     1501 ctaagctatt aaacttcata gttatttttt tgattggata tcaattttat attttattat
     1561 agttatagaa acaataagta acactatttc aaactaaata caaataagtt ttgtatattt
     1621 tacttcacaa aagagctaaa aaattttata tcttaataca acaaataaaa cgtatcttaa
     1681 aataatttag tactaaatca ttttaagata cgttttatct cgtacaagct aaatcatttt
     1741 atcatcttta tcactttgat gttttttatg atggaaatca taattatgat tttcaagatt
     1801 tttatttact ctatttccaa tcttccatga caaaaacacc aataatataa atgatgctat
     1861 ctttaaagga cttgttataa aaccttctat atcacttgct atatagctta taactataaa
     1921 cattacaaat tttcctgcta acattgctgg cacaaaatct tttacatctt ttttaccaaa
     1981 agctgatgcg atagtaacta aacaaccagg cataaatgga caggcatatg ctataaataa
     2041 taatttaaat ccctgtttat ccatccaact tattgctttt tcagttttac tatttctcaa
     2101 tttattaaaa agcttactat cattaaatct gcttattaat aaaaataatg aagctgtccc
     2161 aagtcctgag ccaatccatg ataacaaaca tccaacaaat aatccttgta ttgcagcatt
     2221 tgctgtaact atagcaacaa gtggtaaggc tggtataaag ctttctataa atgcacttaa
     2281 caaacctatt attgtggcta acacccaata attttgagtt aaatcaagta attcttgtat
     2341 atactgcata ttgcttttcc ccttttttaa ctttaactat tattatatac tctatatgtt
     2401 cattatatac taatctcaaa aaatgaatct attaataaaa agtaaataat ctatataaat
     2461 ttacagttaa ggtgtaatta ttttattata tattataaca aaaaagtctt aaaatatgaa
     2521 ttgtcatatt ttaagacttt ttgttctagt atatttaaac tatagtatat cagccttcat
     2581 aatatcctca aaatcacctt tttcagatat taatcttatc aatttaacag tacaacttaa
     2641 atcatttata agtatagctc cgaccaatga atcgtctttc ataaataatt ttttatatat
     2701 tctttgccct tcatcttttg atgaaatttc tttatcaaaa ttttgtatat taccaatact
     2761 aaatactttt acattcattg catctaatga aactggatat acttcacttt caaatgtcaa
     2821 attatctcca cttgcatttg cccctgctat ttttcccatt tctactgaag atggccatat
     2881 tgctaaacta gtattctcca cttgtgcaac atctccacag gcatatatat ctttaacagt
     2941 tgtctgtaat gtcttatcta caacaatacc tctgtcaatt tttacagatg tatcatgagc
     3001 catttgagtg tttgctctta caccaattga aaatactacc atatcacaat ctacactatc
     3061 tccatcttta aatataactt tttgagcttt gccatcacct tcaataccat caactgctac
     3121 acctaatctt acatctattc ctgtatctct tacacattgt tctaatattt tactaccttc
     3181 tttatcaagt tgctttgaca gtatactatc cattgcttca attactacta cttctaatcc
     3241 ttttaatcta aattcccatg cagcttcaag tcctaataaa cctccaccaa tcactacaac
     3301 tttcttagat ttttctagtg cttcttttac actatataaa tcttttatat ttctaagtgt
     3361 aaacacattt tctaaatcat gacctttaat tggtggtata aagtttctac ttcctgtagc
     3421 tattacaagc ttatcaaatt taatagctcc atcattcact ataatagttt tatttacttc
     3481 atctaattta tctacattcg ttcctaatat tactactata ttatttttat cataccaagc
     3541 tttatcttcc atatagaaat ctgagccaag ttcttcattt ataccatcag aaagtgcagg
     3601 tctatagtaa gttaactcat cttcattaga aatcatagtt atcttacaag ttttatttct
     3661 ttttcttatt gcatctgctg cataaaaacc agctgctcca tttcctacta ttacgaagta
     3721 ttcatcagta tcttttctaa aagtaacttc atcttcttca atttctataa attggtcttc
     3781 cttagctcca catacagggc atacactagg agcactatca ccttcaaaaa cttcattaca
     3841 aattacacat ttaaatttct tagttttctt tttacttcca aaagtagcgt ttaacttatc
     3901 taggaactta cctgtataag aatttaattt atcaatctct tcatttgatg gtttaaagtt
     3961 aacggcaaaa ggttttactg ctttcatcct aagttgactt attctctcca tagcattttc
     4021 tataccttct ccactccatc catatgaacc aaatactgaa gcaacttttc caccatgaag
     4081 aactgggttt aacgatacta atatatccca tataggtttt aatgcatctc ctaatattgt
     4141 tggtgttcca aataatacac cttgagatac tgatattttt gctaaaatat cttcttgctt
     4201 atgctctatt acatcaaata aatttacttt atagtcgcta tttttttcaa tatatgcttt
     4261 tatagcttct gccataattt tcgtataacc atgtgcagaa acataacaaa ttgtaacttc
     4321 tttttctaac tttatttgtt catttacact ccaattgtaa taaaggtcta taatttttct
     4381 aggattttca gttaatactg gaccatgacc tggacataca gtgtctatat ctaaatcttt
     4441 tatcttttct atagcagtta ccatagatgg tttaaatggc cccattatac aatcataata
     4501 gtatctcaaa gcatccaggt aatccttttc ttcattttca tcaagtgtat ttactatctt
     4561 atcattacta tagtgactac caaatgaatc acatgttaca agagttttgt cctcttttat
     4621 atatgtgtat atagtatctg gccaatgaag cataggtaca gagaaaaatt ctaaagtttt
     4681 atccccaatt gataatgtat ctccatctgt aactgcaaca tattcaaaat ctttgtttac
     4741 gatttcctta agatatttaa ttgctgtttc tgaagctact acttttgcat ttttagctaa
     4801 atccaaaagc ttttcaacac ttcctgcatg gtctggttca gtatgactta caacaatata
     4861 gtctattttt tcaaaatcta tatttaaatc atttaatctt tcaatataat tgtcaaaatg
     4921 cttatcttta actgtctcaa ataaaactgt tttttctgtt ccttttaata catatgaatt
     4981 gtaagtagtt ccataaggtg tatacataat tatgtcaaat actcttaaat cagggtcaag
     5041 cgaacctacc caatataaat tttcttttat ttttagtgat tttaatgaca taccgtactc
     5101 ctcctaaaat agttatttag tctctttata ccctgcttgt tatattttaa acaaaattta
     5161 ttcaaaaaaa attattttaa agataattta aatcttctaa tcttcttttt ctacctttaa
     5221 tacagttccc tttttagaat ctatttttat gtcatattca tttttatctt tatctattct
     5281 tattttataa aaagtaacat ctaaatcttt atctaaactc cattctttaa ccattccatt
     5341 ttgctctttt aatgcaattt ccatagcttg ttgaggtgta attatgtcat ttaaatctaa
     5401 tttttctttt cttgcaactc catttaaatc ttctgaatcc aaattttctg ttttatcttc
     5461 caatacatct tttgtattag catcaatttt catcttatat tcattattat catctacacc
     5521 ttccacagta taaacaaatt tatttaaatc tctttcaaga cttaaatcta ctacatcagc
     5581 atccttatat ttatctttaa atgtgtttat tgcatctgtg tatggcactt taaaattttg
     5641 ttccattaaa ttttcattat ttttttcatc ttttaaagca ttatttgaag catttgcttc
     5701 cttttttgta ttatcttgtg cttgtcctga atctgcagtt ggactcccac tacatgcagt
     5761 taataaacct gctataagta tacttaccac taataaatac gtttttttac acataaaaat
     5821 cctccttaaa taattaattt attctatatt atgccctcta aaccctttat ttacccttta
     5881 tattaaaaat ttctactatt ttattgattt taaaacttta tacttactaa gattaaagct
     5941 acacaaaaaa gatactgtct caaaatataa acaagtctct ttttcattgt tgaaataata
     6001 aaaatgtatt tgattttaca caaaaagagt gcctcatgaa ataaaaagtt cattttgaga
     6061 cactcatatt ttttatattt ttacttacaa aataacagta cttattattt ttttacaaat
     6121 cttataaaaa tatctatatg atattcttaa atttaaacac tatatcaaag aattaaattt
     6181 tatttaattc ttttactgtt aaatccattt taggatatgg aatcgtaatt ccttcttcgt
     6241 caaacctaac tttaacctct tctagtaaat caagataaat tgtccaataa tcttctgtat
     6301 tgcaccaaac tctagctgca aatttaatgg cattatctcc atgttctact actcctataa
     6361 atggttctgg ttcatttatt attaatttat ggttattaac aatatcttta agtactcttc
     6421 taacatgtag tatatcatct tcatatccta cactaaaagt caagtcaact cgtctagtat
     6481 tctttgctga atagtttatt aggctgtcat ttgatacaga accattaggt ataagtatct
     6541 gtttattatc tggagtaacc aattgtgtat aaaaaagtcc tatttgttca acggttcctc
     6601 catgacccgc tgcttctatg taatctccta ctttaaaagg tctaatcaaa agtataataa
     6661 atcctccagc aaaatttgat aaactacctt gtaaagccaa acctattgct accccggctg
     6721 atgctatcac agcagcaaaa ctggacaaac tcattcccca atattctaga acaatgatta
     6781 taacagcaac tttaagtaca ctcaaaagaa gagacttcaa aaaccttctt aaagttaaat
     6841 caatatctct tttactaagt aatagcataa catggtttac aaattttttt attattttaa
     6901 atccaataga aagtataagt atacctatta taagttttac tccgcttgta gtagcccatt
     6961 ccaacagact ttccattata cctcccatat ccatcttatt aatatctaga ttctttaaat
     7021 ctaacttgct tatatcatct atttttgtcg tatccataaa aaactcctta ccttatattt
     7081 tagaaccaat attatagttt aaagttctta ttttctattt aatataatac aataccattt
     7141 gtgcatagta aacaagattt atattccaat tatacatatt agaaaaatat aaaccacttg
     7201 ttatatatat aaaaaaatat aacattaaat taaactatta gtaaaacact atagtgcctt
     7261 aaaatcatca tttttcacac ctatatgaga tgatactata gttataatac ttagtactat
     7321 aaatactaca aagctttttt tatatccatc aactacatta tatgaattta cttgtaaaga
     7381 gatagctata atcaaattaa taattccact tcctataaat tcaaaagtat taataacact
     7441 agttgccaat ccagaataaa tttctttatt tttatacttg gcatcattaa aaacctgtag
     7501 atgtgacata actatacatc ccataataaa gaatgatatt ggtaaaacta taattggtgg
     7561 ttttacctga tatacaacta caattacaat ccatatgaac aaatctatta ttgctccata
     7621 ttttattata ttaaatttac tagaccttat tttagcaaat acaaaatcca taattaatga
     7681 accaaatata aatccatatg taaaaaatga tactataaac gcagaaaagc tcttactcac
     7741 acaataaacg tccattatgt atcttactcc ccacaagctt ataaatgcac ttgtaagacc
     7801 cacaaaagat aacattatca ttgaattata ccatgttgat ttattcttta taacagactt
     7861 aataccatca acaatattaa ccttctctga ctcttcatat agttcagtat ctacattgaa
     7921 tccatactct tttggtgtat ctctaacaat aatatacata aaacatccaa tcacaacacc
     7981 aattatacca attaataaaa atgagtttct ccaaccaaca agttcagaca ggaacacaag
     8041 tggaaaagta gcaagaactc ctcctaaatt ccctacaaat gaaaatttgg ctgttgcaga
     8101 ggcaaattct gatttattaa accatcttcc ttgaactttt aaaatacaaa gtaatattac
     8161 tgatgttcct gccccaacaa ttacacgaga aatatatgct aattctatac tttgaatcaa
     8221 cccaaataaa attgaaccta aacttgccat tattattcca agactgctaa tttttcttgc
     8281 tccatattta tcaatcaaaa ttcctgcagg tatttgcata aatgcatatg catatagaca
     8341 gaaggatgct atatttgaaa tttgtataga attaaaacct aactcatttt ctaaattgtc
     8401 tgaaacaaca gcagttgaca ttcttaaaaa taatactaaa acaaatgaaa atgtaattat
     8461 accccaaaca ctccacttag cctttgtata acttttcata ccttctcctt aaatccatat
     8521 atttttaatt tttttaatat aacttacaaa acacttgttt tgatattact tatatcaagt
     8581 gtatagttat tcttacaaat ttttacacac aatatcatta tacaacatat ggatatattt
     8641 aactagaaat aaaaccttat tcatattata tgcatgaatt aaatatataa ataataagag
     8701 ccatctttga acattactca aaagatagct ccatataaaa acatttattc taaatttaat
     8761 tttttatcta atataaattt tgtagcgaaa aataatgcta ctataactac aatatcacca
     8821 agtattgcaa acataacatg agaactttga taaaaatgat atatcatatt tgtattttcc
     8881 tttggtaaaa tgcttccaac tgcatctcca acaattgata ttactatatt tatagctata
     8941 aaagaagcaa atgctactat atttctatgt ttattaaatt tgggtaattg tcccattgaa
     9001 attgatgtat ataacaacaa aataaataat gaatatgaag taagcattac caataataat
     9061 actaaaatat ctaaaaagtt ttcagaaatc catttaaaac tagtgttaaa taaatctaaa
     9121 atttcgggta ataaaattcc agaagtacca aaaagcataa gtactccaaa agtgaatatt
     9181 gcaactataa aactaactac agcatatatg agtgctgtta tacatttaga caatataagt
     9241 gaactagtgc taacaggaag agtaaacatt aaataacctt catctcccaa aagatttttt
     9301 ctaaaccttt gaattgtaac tacaatagtt aaaactgcta atgccataaa aagacttgtt
     9361 aatactagta ttgctattcc ctgtatctga agtatatttg tattcataaa tattccatta
     9421 aatacagcta ctatcaatat ggctatatat aacggtataa atactcttcc acttgctttt
     9481 aactcatatt ttaataattt agctaacatt taaattcctc cctaaataat gcatctatag
     9541 attttccttt ttcttctctt attgcttcaa cgtctccttg taacactatt tctccttttg
     9601 atatgaatat tacctcatca catatgtttt ctatttcact tattaagtgg gtagcaatca
     9661 gcaaagtact atcttcagaa taatttttta atatagtctt aagtatatat gaccttgcag
     9721 ctgggtcaac acctcctata ggttcatcca atatatatat acttgcattt cttgacatta
     9781 ctaagataag ttgaaccttt tctttagttc ctttagacat tgtctttagt ttttcattta
     9841 catcaatatc taaactttta atcatctcat ttgctcttct aacatcaaaa tcactataaa
     9901 agtcatagaa aaatttaagc aaatcagata ccttcatcca atcatttaaa taagttcttt
     9961 ctggtaaata tgaaactatt tttttagtct ctatggatgg tttcattcca tttatcataa
    10021 tttcaccatt atcaggctgt aacaatccat tcattaactt aatcattgta cttttaccac
    10081 ttccattagg tcctaacagc ccaactattt ttccctttgg tatatttaaa tcaatattct
    10141 ttaaaacagt cttaattcca tagctcttgt ttacaccttt aaattcaact attccatctt
    10201 gagaaatatt atttatattt ttatccattt tataattctc ctttcagatt ttctgtaata
    10261 agttttagta tttcatctct atcatatcca agcttttcta aagttacttt taatgtattt
    10321 atttccacat tagctatttc ctctttcata gcttttattt tttccttatc atctgttaca
    10381 aatcttcctt tggttctctg actataaact aaaccttgtc tttcaagttc tgttaaagcc
    10441 ttttgcattg tatttggatt aacttcagca tcttctgcta gtgctctcac ggtttctaat
    10501 ttagatccta ttttaatttc tcctgaaatt atttttaact ttaaatgttc tactaattgt
    10561 atatatatag gtttgttatt atcaagttcc cattccatat tcatacctcc tttttacact
    10621 attgtattag ttcgttaata caataatata ataatacaga gtatatgtca atgactcttt
    10681 aataaaagca taaaaaattt tacaaaaaaa aatgaacact acattagagt ccatttttaa
    10741 cacaatttac taaattttta taatacagat taattaccct cttatcaaat tagatacaat
    10801 taaatgttca aattcttcaa ttggcatagg ctttccaaat aaatatcctt gtgccatatc
    10861 acattttact tcctttaaaa gttcaaactg ttcttctgtc tcaactcctt ctgatatgac
    10921 tttcatacct aactcttttg ccatttctac taccttagta acaattattt tgcttctttt
    10981 actattataa gtttccataa taaatttttg gtcaagtttt aatatatcca taggaatttc
    11041 ttttaacata tttaatgatg agtatcctga accaaaatca tccatagata tcaaaaatcc
    11101 tatttttttt aaattattca ttatgtcaat taaaatatca aaattatcaa atatgatact
    11161 ctctgttaat tcaagttcta tatattttgc aggtatgtta tatttatcaa ttatattttt
    11221 gtatgtttct ataaaattat tgcaatataa atgtattctt gaaacattaa ctgaaatggt
    11281 aactggattt attcctgcat caatccattt tctaaggtat atacatactt cttcaagcac
    11341 atataaatct aaatttataa caaaaccgtt tttttcaaaa aatggtataa attcatttgg
    11401 aggtattaat cctttatcag ggcgtttcca cctaacaagt gcttctgaac cttgtatttc
    11461 tcctgtatta agctctatct taggttgtaa gtatataata aattctttat tttctaaact
    11521 tgaaaacatt gtattttcca tctctttttc ttttatctct tggtcatgaa tcttattatc
    11581 ataaaatgca tatgaacttt tatgtcctcc ctttgccatt ttatgaggta tattagctct
    11641 atctatgatt gagttgatat ctctatcttc tggaagtata aaatagattc cacaacttaa
    11701 aactaactta tacttttctt cctgattatt attaaacaag tctagttctt caaatataat
    11761 ttttaacctg tttgttatat cttcttcttc tatatatttc ataattataa taaaattatc
    11821 cgcagatatt cttgcaaaca tttcctcttc aaatatatta ctcttaataa tctttgttaa
    11881 attacgcaaa atttcattcc caacttcata tccaaatagg tc
//
LOCUS       DAFNNB010000122        11784 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0123, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000122 DAFNNB010000000
VERSION     DAFNNB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0123"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..151)
                     /locus_tag="L3M60_002721"
     CDS             complement(<1..151)
                     /locus_tag="L3M60_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6227364.1"
                     /translation="MTRISNITIAEQSEYCILAIRKTIDFVVEFSEFSKQSLEKISKY
                     LEERGI"
     gene            complement(461..1882)
                     /gene="nhaC"
                     /locus_tag="L3M60_002722"
     CDS             complement(461..1882)
                     /gene="nhaC"
                     /locus_tag="L3M60_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBN6227365.1"
                     /translation="MKERECRKASLFDAMIPITCLILFLSLGVLVYGSSPHVPLIGAA
                     AVAGLVAVYRLGFKWKELELSMFNSIKMAMQAILIIIIIGVLIGTWIVSGVVPCMIYW
                     GLKILSPNVFLVASTLVCAIVSLATGSSWSTMGTVGVALLGIGQSLGMPVGLIVGSII
                     SGAYFGDKLSPLSETTNLAPAMAGTDLFTHIKYMLYSTIPSLLICLVIYGVIGMKYSG
                     QALDIKQIELIRSTLDSTFNTLSPVLLLAPAIVIGLVVFKVPAIPGLIVGAVLGVLFA
                     VVFQGESMNTILDAAQNGYVSSTQIQEVDALLSKGGIMNMMSTVSLTICALSLGGILE
                     KTGMLEVVASSLLKLAKGVFGTVLCTMVTCTITNIIAGEQYLSIVIPGRMYNKEYKKR
                     GIHPKMLSRALEDSGTLTSPLVPWNTCGAYITATLGVSALTYGPYALLNIINPIVSLV
                     LIACKFKIARIEDEPETCLNFDI"
     gene            complement(1912..2976)
                     /locus_tag="L3M60_002723"
     CDS             complement(1912..2976)
                     /locus_tag="L3M60_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HBN6227366.1"
                     /translation="MYPRLEIDIEKLKHNAKLISQMCHERNIKISFVTKSFCAQKEVV
                     KAICSEGIDHIADSRIQNLKNLQDINLPKILIRIPMLSELEEVINYCDISFNSELGTI
                     KKLNELCESKNIIHKIVLMFDLGDLREGYFYEEDFFYNVREIIKLKNIKIIGIATNLT
                     CYGAIIPSRENIGRLASIAQKMEGKFGINLEIVSGGNSSSIHLMTNDNMPEGITNLRI
                     GESILLGRETAYGENINGTYQDAFKLICQVVECKEKPSVPIGEIGFDAFRNKPVYEDK
                     GILKRAIIAIGKQDINIDSLIPIDTDIKILGASSDHMILDVSNTKYDYKLGDSVEFLL
                     TYGGIMSSSTSKYVSKKIIV"
     gene            complement(2961..4370)
                     /locus_tag="L3M60_002724"
     CDS             complement(2961..4370)
                     /locus_tag="L3M60_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HBN6227367.1"
                     /translation="MENSKVSLKIDVLVAEIGSTTTVVNAFHNIDTNNPVFLGQGQAP
                     TTVFEGGDVRNGLSGAIKDLANKLSVDNIEYNDMFATSSAAGGLKMTVHGLVYDMTVK
                     AAKEAALGAGAVIRQVTSGRIKRTDLNKIKEINPNIILIAGGVDYGERDTAIYNAEMI
                     ASMNLGIPVIYAGNIENQEEVRLIFEDTNYKLYITENVYPKIDLLNIEPTRRIIQSVF
                     EEHITTAPGMKYIKEMVNENITPTPGAVMEASKLLYKNIGDLLTLDVGGATTDVHSVT
                     DGSDYINKILVNPEPTAKRSVEGDLGVYVNMKNIVEVIGKENLQSELSIDIDAVIENY
                     PPIPKSKEEILFVERLTKEAVVKAMLRHSGKIRNIYGTTGKIKIAEGKDLTEVRYIVG
                     TGGALTRLPSRIEILDNMLKYNKNNELLFPKEKTKILIDNDYIMASMGVLSKKYEEAS
                     LKLLLKSLNFEEERLCILG"
     gene            complement(4370..6574)
                     /locus_tag="L3M60_002725"
     CDS             complement(4370..6574)
                     /locus_tag="L3M60_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892628.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HBN6227368.1"
                     /translation="MLKENKKLDIDYILKDLDKYKPKRRGWVWREHLENLEMGPFKYR
                     DCTKPLKDSVGLPSSKYFNNIDPQPSPVITTEIASGRFEDDIRRMRMAAHHGADHLMV
                     IRTAGQSHFDGLIEGTPQGIGGVPITRKQVRAQRKALDFIEEEVGRPINYHSYVSGVA
                     GPEVAVMFAEEGVNGAHQDPQYNVLYRNINMIRSFVDACEAKKIMAFANIAQIDGAHN
                     ANATAREAWKVMPELMVQHALNSIFSEKIGIDKSNICLSTVPPTAPPAPCLRIDLPYA
                     VALRELFSDYKMRAQMNTKYMESSTREATVTHVLNLLTSVLTRADIQSTITPDEGRNV
                     PWHIYNIEACDTAKQALVGMDGLMDMIELKDVGELREKARELKERAVLYMEEIFEVGG
                     YFESVEQGFFVDSGNYPERNGDGISRKIKGGVGEGTVYEREEDYLAPVTAHFGYNNVA
                     QYDKNAIDNPSILIDGCTFENPDKIIYIDELDDFDNVENRLKESDEYRNGTKIKPEME
                     WCADGIVMITMMLPTDKRTAEFAALEFVKKMNLQETEVISREVMHESEGTRIEVKGRV
                     PFDIDLKNLVIPEEPKVLTDEEIRADIEEKPMKIVSATVGEDEHSVGLREIIDIKHGG
                     IEKYGIECHYLGTSVPVEKLVDAAIELNADAILASTIISHDDIHYKNMKKLHDYCVEK
                     GIRDKVMIACGGTQVTPEIAVEQGIDAGFGRNSKGIHVATFLVEKRREMNNK"
     gene            complement(6568..6942)
                     /locus_tag="L3M60_002726"
     CDS             complement(6568..6942)
                     /locus_tag="L3M60_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HBN6227369.1"
                     /translation="MKKREDDFEVRRKHLQELSDDELKERFWSLATQIVEPMIELGKK
                     NTTPSIERSVLLRMGFSSLEVKPILEGVMERGLIGKGAGHVVYKLAKSKNITVREAGL
                     LLVNGEYWDEVTCLFREGVESC"
     gene            complement(7020..8450)
                     /locus_tag="L3M60_002727"
     CDS             complement(7020..8450)
                     /locus_tag="L3M60_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent lyase/thiolase"
                     /protein_id="HBN6227370.1"
                     /translation="MNNSASMKDMSYQAVMGRNNEIMKNAIGLDYSSFEQEGIGFDYE
                     KMMSETGYTLQDIEVIQSQYAVGNTPIIELKNLTKLARKCAKEGKGARIFVKDEAMNA
                     SGSFKARRAATAVYHAKKMGYKGVIAATSGNYGAAVASQAAMQGLKCIIVQECYDSNG
                     VGQPEIIEKARKCEAYGAEVVQLTVGPELFYTFLVLLEETGYFNASLYSPFGIAGVET
                     LGYELAIQFREKYKKDPDIVVCTNAGGGNLTGTARGLIKAGSINTKVVGASVDLKGLH
                     MASDNQFNKKSFTTGHTGFGIPYCTWPDRSDVPRSAARPLRYMDRYVLVKQGEVFYTT
                     ELLAQLEGIERGPAGNTSLAAAFSLAQELDEDKVIVVQETEYTGAGKHICPQLTFARE
                     NGIDIKFGNPREEIAGVNLILPERPELLKCVDIDMNKIRKSFIKNCISNNHIDNIDKL
                     SNRDIEFLMEEVKSSRDFVIDVLNSL"
     gene            complement(8443..8751)
                     /locus_tag="L3M60_002728"
     CDS             complement(8443..8751)
                     /locus_tag="L3M60_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-ketopentanoate thiolase"
                     /protein_id="HBN6227371.1"
                     /translation="MDAKINDWVIIHNIVLTPEERAPQVPEDTKKVSLEMWVKGFIQS
                     DASIGDLVEVKTITGRLVKGNLLKVNPYYTHDYGKCIPELLQIGIQAKEILFGGVSNE
                     "
     gene            complement(8849..9916)
                     /locus_tag="L3M60_002729"
     CDS             complement(8849..9916)
                     /locus_tag="L3M60_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-binding protein"
                     /protein_id="HBN6227372.1"
                     /translation="MRKVRVGIWGFGAMGIGMANMILKKEGIEIVSVCSRSTSGKSMY
                     DVLGIERGERPEVIINKNYEEVFREKSVDVVLLATDSFTKKAFDKILFLLNRKINVIS
                     TAEQMAYPQADDVDLAKKMDEVAKENGVSILGTGINPGFVLDLLVLALSGTCEEVTSI
                     KAKRVNDLSPFGKSVMVEQGVGATREEFIKGVEDKTIAGHVGFVESINMIADGLGWRL
                     DKIEQTKEPIMTTVDRKSKYGEALAGNVAGCRQCGYGYVNGEVLIEMEHPQQIIPEAE
                     GIKTGDYVSIKGIPNIDLQINPEIPGGVGTYAMIVNSIPLIINARPGLKTMLDIPVPR
                     AIMGDIRNQIEVELEEESKAN"
     gene            complement(10176..11549)
                     /locus_tag="L3M60_002730"
     CDS             complement(10176..11549)
                     /locus_tag="L3M60_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6227373.1"
                     /translation="MNEILENIDEGVHFVNKDGKTIIYNNSVQKMEKMNRHDILTNSF
                     FDIVNKMKINRSTLLEVLEKREIIKDNIQKYLDKNGKEITAINTTIPIDIKGEFVGAL
                     EISKNMTTIENLLNRNYKKNLVNKVSSRQKKGYEFDDILGESNLIKEAIEKSKKACKS
                     DASVFIYGETGTGKELFSQSIHYGSNRRNYPFIAQNCAALPESLFEGILFGTSKGGFT
                     GAIDRPGLFEQANKGTLLLDEINSMPIMLQAKLLRVLQEGYVRRVGGTKDIPIDVRII
                     ATTNESPKVILNEHKMRKDLYYRLNIIHIKIPPLRDRKEDIPLLCKKFISKYNKKLNK
                     RVIGISDEAIKILKNYDWQGNIRELENIIYCTMSMMEDEVQIETSMINLNDYYNLKEN
                     KESKNYSLNDLEDKTLSKIISEIEEKYIYESLEKTSYNITKAASILGMNRQNLQYKIK
                     KYNIKIL"
BASE COUNT         3511 a         2318 c         1275 g         4680 t
ORIGIN      
        1 agattcctct ttcctccaaa tatttagaaa ttttttcgag actttgcttt gaaaactctg
       61 aaaactctac cacaaagtca attgtttttc gtattgctaa aatacaatac tcagattgtt
      121 cagctattgt tatattagat attctagtca ttttattcct ccattacttt atttttacct
      181 ctaatccttt tataccaaat tacacaaaat ttcgctgtat aaataattat ttcatatgaa
      241 tataaaacat agcctccttc catttcttat attgaaagta taacaaaaat aatcttatga
      301 tgtttgtcac acagtgctct tatagtagta catatttaaa agacttgcct aattttaagg
      361 caagtctaaa agaaatcttc tttctataaa aaatttatac aaacaattca aatatcgaaa
      421 tataagctac tatcaaacta taaatttaaa tttactaata tcaaatatca aaatttaaac
      481 aagtttcagg ctcatcttct attcttgcta ttttaaactt acatgcaatt aataccagag
      541 atactattgg atttataata tttaacaaag catatggacc atatgttagt gcagatacac
      601 ctaatgttgc tgttatataa gccccgcatg tattccatgg tactaatgga gaagttaaag
      661 ttccactatc ttctaaagct ctcgacaaca tcttaggatg tatacctctc tttttatatt
      721 ccttattata cattcttcct ggtataacta tagatagata ttgttcacct gctattatat
      781 tggtaattgt acaagttacc atcgtacata atactgttcc aaatacaccc ttagctaatt
      841 ttagtaaaga agatgctact acttcaagca tacctgtttt ttctaaaata cctccaagag
      901 ataaagcaca tattgttaaa gacacagtgg acatcatatt cataattccg cctttagata
      961 ataaagcatc tacctcttgt atttgtgttg acgaaacata tccattttgt gctgcatcta
     1021 atatcgtatt catagattct ccttgaaaaa ctactgcaaa caatactccc aatacagcac
     1081 ctactataag ccctggtata gcaggaactt taaatacaac taatccaatt actattgccg
     1141 gagctaataa taatactggt gataaagtat taaatgtaga atctaatgta gacctaatta
     1201 actctatctg ttttatatct agcgcttgac ctgaatactt cattccgatt acaccataaa
     1261 ttactaaaca aattaataat gatggtatag tcgaatatag catatatttt atatgtgtaa
     1321 ataaatctgt tcctgccatt gctggagcta aattagtcgt ttcagataaa ggtgatagtt
     1381 tatctccaaa gtaagctcca gatattatag aacctactat tagtccaact ggcataccta
     1441 aactttgacc aattcctaaa agagctactc ctactgtacc cattgtactc caagatgaac
     1501 ctgtcgcaag agatactatt gcacagacta gtgttgatgc tactaaaaaa acatttggtg
     1561 ataaaatttt tagtccccag taaatcatac atggaactac tccacttact atccaagttc
     1621 ctataagtac gcctattata attatgatta agattgcctg cattgccatc tttatactat
     1681 taaacatact taattctaac tctttccatt taaatccaag tctatataca gccacaagtc
     1741 ctgccactgc tgcagctcct attagtggta catgaggtga tgaaccatat actaatactc
     1801 ctagacttaa aaataatatc aaacaagtaa ttggtatcat tgcatcaaac agacttgctt
     1861 ttctacattc tcgctctttc ataaaataat ttcctcctaa ttatttaaac actacactat
     1921 aatttttttt gaaacatatt tactcgtact agatgacatg ataccaccat aagttaataa
     1981 aaattctaca ctgtcaccta atttataatc atattttgtg ttgcttacat ctagtatcat
     2041 gtggtctgag cttgccccta atatttttat atcagtgtct attggaatta aagagtcaat
     2101 atttatatcc tgtttaccga tggctataat tgcccttttt aagattcctt tatcttcata
     2161 aacaggctta tttctaaaag catcaaatcc tatttctcca ataggaactg atggcttttc
     2221 tttacattca actacttggc atattaattt aaatgcatct tgatacgttc catttatatt
     2281 ttcaccgtat gccgtttctc taccaagtag aatagattcg cctattctca gattagtaat
     2341 tccttctggc atattatcat ttgtcatcag atgtatagaa cttgaattgc ctccagatac
     2401 tatttctaga tttattccaa atttgccttc cattttttgt gctatacttg ctagtctacc
     2461 tatattttct ctagagggta taatagctcc ataacaagtt aaatttgtag ctataccaat
     2521 tattttaata ttttttaatt ttattatctc tctcacatta taaaaaaaat cttcttcata
     2581 aaaataccct tctcgtaaat ctcctaagtc aaacataagg actatcttat gaataatatt
     2641 tttactttca cataattcat ttaatttctt tattgttcct aactctgagt taaaactaat
     2701 atcacaataa ttgataactt cttctaattc acttaacatt gggattctaa tcaatatctt
     2761 aggtaaattt atgtcttgta aattttttaa gttttgtatt cttgaatctg ctatatggtc
     2821 tattccttca ctacatattg cttttactac ttctttttgt gcacaaaatg atttagtgac
     2881 aaaagatatc ttaatatttc tttcgtgaca catctgcgaa attaattttg cattatgctt
     2941 taacttttct atatctattt ctaacctagg atacataatc tttcctcctc aaaattcaaa
     3001 ctttttaata ggagcttaag agatgcttct tcatattttt tagataaaac acccatagat
     3061 gccataatat aatcattatc tatcaatatc tttgtttttt cctttggaaa tagtaattca
     3121 ttatttttat tgtatttaag catattatct aatatttcaa ttctactagg gagcctagta
     3181 agtgctcccc ctgtaccaac tatatatctt acttctgtta aatctttccc ttctgcaatt
     3241 ttgattttcc cagttgttcc atagatattt cttatttttc ctgaatgtct taacatagct
     3301 ttaacaacag cttcttttgt taatctctca acaaataata tttcttcttt tgactttggt
     3361 attggaggat aattctctat tactgcatca atatctatag ataattcact ttgtaagttt
     3421 tctttaccta tcacttccac tatattcttc atatttacat atacacctaa atcaccctca
     3481 acactcctct ttgcagtagg ttcaggatta actaaaattt tattaatata gtcacttcca
     3541 tctgtaacag aatgtacatc agtagttgct ccacctacat ccagagttaa taaatctcca
     3601 atatttttat ataagagttt agaagcttcc attacagctc ctggggttgg agtaatattt
     3661 tcattaacca tttctttaat gtatttcatt cctggtgctg tagttatgtg ttcttcaaat
     3721 acactttgaa tgattcttct tgtaggttct atattcaata aatctatttt agggtataca
     3781 ttttcagtta tataaagctt ataattagta tcttcaaata ttaaccttac ttcctcttga
     3841 ttttcaatat tcccagcata tataactggt atgcctagat tcatagaagc tatcatttct
     3901 gcattataaa tagctgtatc tctttctcca taatcgactc caccagctat taaaattatg
     3961 tttgggttta tttcttttat tttatttaaa tcagttcttt taattctacc tgatgttact
     4021 tgacgaataa cagctccagc ccctagggca gcttctttag ctgcttttac tgtcatatca
     4081 tagactaatc catgaacagt catttttaat ccaccagctg cactagaagt tgcaaacata
     4141 tcattatact ctatattatc tacagataat ttattagcta aatccttaat agcacctgat
     4201 aaaccgtttc ttacatcgcc accttcaaac actgttgttg gagcttgacc ttgacccaaa
     4261 aatacaggat tgttagtatc aatattatgg aaagcgttta ccacagtagt agtgcttcca
     4321 atttcagcta ccaacacatc tatttttaag gaaactttgc tattttccat tatttattat
     4381 tcatttctct tcttttttca actaagaaag tggctacatg tatgcccttg ctgtttcttc
     4441 caaatccagc atctattcct tgttcaacgg ctatttcagg tgttacttgt gtccctccac
     4501 acgcaatcat tactttatct cttatacctt tttccacaca ataatcgtgt aatttcttca
     4561 tgtttttata atgaatatca tcatgactta ttattgtaga tgcaagtatt gcatctgcat
     4621 tcagttcaat tgctgcatct accaattttt ctacaggtac agaagtccct agatagtggc
     4681 attctatacc atatttttca ataccaccat gttttatatc tattatttct cttaaaccaa
     4741 ctgaatgttc atcttctcca accgtagctg aaactatttt cataggtttt tcttctatat
     4801 ctgctcttat ctcttcatca gttaatacct taggttcttc tggtattact aaatttttta
     4861 aatctatatc aaatggaact cttcctttca cttctatcct agtcccttca gattcatgca
     4921 taacctctct acttataacc tcagtttctt gtaagttcat ttttttgaca aactccagag
     4981 ctgcaaattc agctgttctt ttatccgtag gaagcatcat tgttatcatt acaattccat
     5041 ctgcacacca ttccatttca ggcttaatct tagttccatt tctatattca tctgattctt
     5101 ttaatctatt ctctacatta tcaaaatcgt caagctcatc aatatatatt attttatctg
     5161 gattttcaaa tgtacatcca tctattaata ttgatggatt atctatagca tttttatcgt
     5221 attgggctac attattgtat ccaaagtgag cagttacagg tgctaaataa tcttcttctc
     5281 tttcataaac agtaccttca ccaactcctc cttttatttt tctagatatt ccatcaccat
     5341 ttctttctgg ataatttcct gagtcaacaa agaatccttg ttctacagat tcaaaatatc
     5401 cgccaacttc aaatatttct tccatatata atacagctct ttctttaagt tcccttgctt
     5461 tttctcttaa ttcaccgaca tctttaagtt ctatcatatc cataagacca tccataccaa
     5521 ctaaagcttg ctttgctgta tcacacgctt ctatattata tatatgccaa ggtacatttc
     5581 taccttcatc aggtgttata gttgattgta tgtcagctct agttaatact gatgttaata
     5641 agtttaatac atgagtaact gttgcctctc tagttgatga ttccatatat ttagtattca
     5701 tttgagctct catcttataa tcactaaata actctctaag tgcaacagca taaggtaagt
     5761 ctattcttaa acatggtgct ggaggtgctg ttggtggaac tgttgataag catatattac
     5821 ttttatctat tcctattttt tctgagaata tagagtttaa agcgtgttga accataagtt
     5881 caggcattac cttccatgct tctctagcag tagcattagc attatgcgct ccatctattt
     5941 gagctatatt agcaaaagcc attatttttt tagcttcaca agcatctaca aaagacctta
     6001 tcatatttat atttctatat agaacattgt attgtgggtc ttggtgtgct ccattgactc
     6061 cttcttcagc aaacattact gctacttcag gaccagcaac accacttaca tacgaatgat
     6121 aattaatagg tctaccaact tcttcttcta taaaatcaag agcctttctt tgagctctta
     6181 cttgttttct tgttattgga actccaccta tcccttgggg agttccttct ataagcccat
     6241 caaaatgtga ttgcccagca gttctaataa ccattaaatg gtcagctcca tgatgagctg
     6301 ccatcctcat tcttcttata tcatcctcaa atctaccaga agctatttca gtagtaataa
     6361 ctgggcttgg ttgagggtct atattattga aatacttaga tgatggtagc cccacactgt
     6421 cttttaaagg ttttgtacag tctctatatt taaaaggtcc catttcaaga ttttccaaat
     6481 gctctctcca aacccaacct cttctttttg gtttatattt atccaaatct tttaatatat
     6541 agtctatatc caatttttta ttttccttta acatgattct accccttctc taaataaaca
     6601 tgtaacttca tcccaatatt caccattaac taatagtaaa cctgcttctc taactgttat
     6661 attcttagat ttagccaact tataaactac atgccctgct cctttaccta taagacctct
     6721 ttccataacc ccttccagaa taggttttac ttcaagagaa gaaaatccca ttcttaacaa
     6781 gacagacctt tctattgatg gtgttgtatt ctttttacca agttctatca ttggttctac
     6841 tatctgggta gctaaactcc aaaacctttc ttttaactca tcatcactca gttcttgaag
     6901 atgttttctt ctaacttcaa aatcatcctc tctttttttc atgaacattc ctccattcgt
     6961 attgatttaa tcgaattttc cgaattttta ttttacatta aatcaaaaaa caacaactac
     7021 tataaactat ttaatacatc aataacaaaa tctcttgaac tttttacttc ttccattaaa
     7081 aattctatat ctcgattact tagcttatca atattatcaa tgtgattatt ggatatacaa
     7141 ttttttataa atgactttct tatcttattc atatcaatat caacacattt caataattct
     7201 ggtctttctg gtaatattaa gtttactcct gcaatttctt ctcttggatt tccaaatttt
     7261 atatctattc cattttctct tgcaaaagta agttgtggac atatatgttt acctgcacct
     7321 gtatattctg tttcttgtac cactataact ttatcctcat ctaactcttg agctaaagag
     7381 aatgctgctg ctaaagatgt atttcctgct ggacctcttt ctataccttc tagctgtgcc
     7441 aataattcag ttgtataaaa aacttctcct tgttttacta acacatatct atccatatat
     7501 ctaagtggtc tagctgcaga cctaggtaca tctgacctat caggccatgt acagtatggt
     7561 ataccaaaac ctgtatgtcc tgttgtaaat gattttttat taaattgatt atcgctagcc
     7621 atgtgtaacc cttttaaatc tacacttgct cctactactt tagtattaat agaaccagct
     7681 tttatcaatc ctcttgcagt tccagtaaga ttaccaccac ctgcatttgt acaaacaact
     7741 atatcagggt cttttttata cttttctcta aattgtatag ctaactcata acctaaagtt
     7801 tctacacctg caattccaaa tggtgaatat aatgatgcgt taaaatagcc tgtttcttct
     7861 aataaaacta agaacgtata aaataactct ggtccaacag ttaactgaac aacttctgct
     7921 ccatatgctt cacattttct tgctttttct ataatttcag gctgacctac accattagaa
     7981 tcataacact cttgaactat tatacatttt aatccttgca ttgcagcttg ggatgctaca
     8041 gctgcaccat agttaccact agtagctgct attacacctt tatatcccat ttttttagca
     8101 tgatatactg cagtagctgc tcttcttgct ttaaaactac cagatgcatt cattgcttca
     8161 tcttttacaa atattctagc tcctttacct tctttagcac attttctagc taatttagtc
     8221 aaatttttaa gctctattat aggagtattt ccaacagcat attgtgattg aattacctca
     8281 atatcttgta atgtatatcc tgtttcactc atcatcttct cataatcaaa ccctattcct
     8341 tcttgttcaa aagaagagta atccaatcca attgcatttt tcattatttc gttatttctt
     8401 cccataacag cttgataact catatctttc attgatgctg aattattcat tagatacacc
     8461 tccaaataat atttctttag cttggattcc tatttggagt aactctggta tacatttacc
     8521 ataatcatga gtataatatg ggtttacttt aagtaaatta ccttttacaa gtctacctgt
     8581 aattgtttta acttctacta agtcgcctat agaagcatca ctttgtataa aacctttaac
     8641 ccacatttct aaggatactt tcttagtatc ctctggaact tgtggagctc tttcttctgg
     8701 agtcaatact atattgtgta taataaccca atcattaatt ttagcatcca ttttttcatc
     8761 cccttattaa ttgactattg tcttactaat taatttactg ttattttaaa ttaactatta
     8821 ttttaccaat tagttagctg ttatcttact aattagcctt cgattcttct tccaattcaa
     8881 cttctatctg atttcttata tctcccatta ttgctctagg tactggtata tccaacatag
     8941 ttttaagacc tggtctagca tttattatta atggaataga gtttactatc attgcataag
     9001 tacctactcc tccaggtatt tcaggattta tttgtaaatc aatgtttggt atacctttta
     9061 ttgatacata atcgcctgtt ttaatgcctt ccgcttctgg tattatctgt tgtggatgct
     9121 ccatttcaat taaaacttct ccatttacat atccatatcc acattgtcta caaccagcaa
     9181 catttccagc caatgcctct ccgtacttag attttctatc tactgtagtc attattggtt
     9241 cttttgtttg ttctatctta tctagtctcc atcctaatcc atccgctatc atatttattg
     9301 attctacaaa acctacatga cccgctatag tcttatcttc tacacctttt ataaattctt
     9361 ctcttgttgc tccaacacct tgttctacca taacagactt tccaaatgga gataaatcat
     9421 ttactctctt agcttttata ctagtaactt cttcacaagt acctgaaagt gctaatacta
     9481 gtaaatctaa tacaaaccct ggatttattc ctgttccaag tatacttact ccattttcct
     9541 ttgcaacttc atccattttt ttagctaaat ccacatcatc agcttgtgga tatgccattt
     9601 gttctgctgt tgatataaca tttatttttc tatttaataa gaatagtatt ttatcaaaag
     9661 cctttttagt aaatgaatca gttgccaata aaacaacatc tacgcttttt tctctaaaaa
     9721 cttcttcata atttttattg ataataactt ctggtctttc tcctctttca attcctaaga
     9781 catcatacat acttttccct gaagtacttc tactacaaac agatactatt tctatacctt
     9841 ctttttttag aatcatatta gccataccta ttcccatagc tccaaatccc catattccta
     9901 ctcttacttt tctcatatat acaccctcct aaaaatttat atattctcaa taattagtta
     9961 aagcataaat cgtgccaact ttaaaattaa aataaatatt tattagagtg ttgaaactat
    10021 aattacttta ttacgtattt tcataattaa aaaataatat ttcttctgaa aaataaaaaa
    10081 aagagcaaaa tattttgctc ttttataata attttgctcc tttattttgc ttttttactt
    10141 tactttgatt gttcctctat tttgctcttt tttatttata atattttgat attatatttt
    10201 tttattttat actgtaaatt ttgcctattc atacctaaaa ttgatgcagc tttagttata
    10261 ttgtatgatg tcttttctag cgattcatat atatattttt cttctatctc actaataatc
    10321 ttactaagtg ttttgtcctc caaatcattt aaagaataat ttttactctc tttattctct
    10381 ttaagattgt aatagtcatt taaattaatc atactcgttt ctatttgtac ctcgtcttcc
    10441 atcatactca tagtacaata aattatgttt tctagctctc ttatgttacc ttgccaatcg
    10501 taatttttta aaattttaat agcttcatct gaaatgccta ttactctttt atttaacttt
    10561 ttattatatt ttgaaataaa ttttttgcac aatagaggta tgtcctcttt tctatctcta
    10621 agaggaggaa tttttatatg aattatgtta agcctataat ataaatcttt tctcatttta
    10681 tgctcattta aaatgacctt tggactttca tttgtcgttg caataattct tacatctata
    10741 ggtatatcct tagttcctcc aactcttctt acataacctt cttgtaaaac ccttagtaat
    10801 ttagcctgta acataatggg catagaattt atttcatcta ataacaaagt tcctttgtta
    10861 gcttgctcaa ataagcctgg cctatctatt gcccctgtaa atcctccctt gctagtccca
    10921 aaaagtatac cttcgaatag tgattctggt aaagctgcac aattttgtgc tataaatgga
    10981 tagtttctcc tattacttcc atagtgaatt gactggctaa ataactcttt gcctgttcca
    11041 gtttcaccat atataaatac tgaagcatca gacttgcaag ctttcttaga tttttctatt
    11101 gcttctttaa tcaaattact ttcccctaaa atatcatcaa attcatagcc ctttttttgt
    11161 ctactactaa ccttattgac taaatttttt ttatagtttc tatttagtaa attttctata
    11221 gttgtcatat tttttgatat ttcaagtgcc cctacaaact ctcctttaat atcaattggt
    11281 attgtagtat taattgctgt tatttctttt ccatttttat ctagatattt ttgaatattg
    11341 tctttgataa tttccctttt ttccaaaacc tctaatagtg tacttctgtt tattttcatt
    11401 ttgtttacta tatcaaaaaa tgaattggtt aaaatatcat gtctattcat tttttccatt
    11461 ttttgtacag aattattgta gattatagtt ttcccgtctt tattaacaaa atgtacccct
    11521 tcatcaatat tttccaatat ttcattcatt attttaaaaa atatctcttg gttcaactat
    11581 atcaccccag tacctaaata tataaaaacc caaaaaatta ataaaatatt tccattgtat
    11641 ttatcttatc atatatttat gatatttttt atgtacatta tattaatata tttttaagta
    11701 tatttaaaat tttatttata tatctttcat tatggttata caatagttga attgtcatta
    11761 aatttgccaa tagttttata tatt
//
LOCUS       DAFNNB010000123        11552 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0124, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000123 DAFNNB010000000
VERSION     DAFNNB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0124"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            713..1915
                     /locus_tag="L3M60_002731"
     CDS             713..1915
                     /locus_tag="L3M60_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBN6227374.1"
                     /translation="MKASILITLIEKTSLIVVLFLLITKLKIFKQIFQKEEYSFNDLV
                     CIALVFTFLAIFGTYSGINYMGSIVNTRIISIVSGGILFGPMVGITAGVFSGIHRYFM
                     DIGGITSVPCLLSSILAGVLSGFLYKRIPKQHRVMYGILVGMISESFTILLIYLISYP
                     HSLAIQIIGGIYLPLIVGQIGIGFVISIVEGIEKDKKDIEARNKAEIKALQRQINPHF
                     LFNSLNTIASFIRFSPDKARELIINLSTYLRYNLEYSDNLIDINKEIEQVKSFVEIEK
                     ARFGELLTVSYDIEDVNIKIPSLIIQPLVENAIIHGILESGRAGAVKISIKKLPYSLE
                     NTVRISIEDNGIGISEEIINNVYQDNMPENKIGLYNVHLRLKLMYGRGLNIRRIDNGT
                     LIVFYVKE"
     gene            1920..2690
                     /locus_tag="L3M60_002732"
     CDS             1920..2690
                     /locus_tag="L3M60_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227375.1"
                     /translation="MRAIIVEDEFPARKELRYFIENKSSIEVVSEFTNGIEVLDFIQE
                     NKIDVIFLDINIPHLDGMLLAKTLNQFKSRPKIVFITAYENYAVDAFSLDVFDYILKP
                     YSEERIISMLNKLEKSERSDIELSSGNSNLYKYKKETVNQDIEEITHKISLWKGDKLV
                     VIDIDDIYYCEANERQTFIYTEKEKFILKEGISEVENLINDKTFFRTHRSYIVNLTKV
                     KEIIPWFNNTYILKLKNNDYEVTVSRSKVKEFRLLMHI"
     gene            2836..4257
                     /locus_tag="L3M60_002733"
     CDS             2836..4257
                     /locus_tag="L3M60_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HBN6227376.1"
                     /translation="MVSFLGSIVVLIVGYFIYGTFVEKVFGINDKNQTPAIACRDGVD
                     YVPMKWKRIFLIQFLNIAGLGPIFGAIQGALFGPSAFLWIVFGTIFAGGVHDFASGYL
                     SMKNKGTSASELVGIYLGNGAKIAMRIFSVILLVLVGVVFVTGPAGLLKTLTGVNAQI
                     WVGVIILYYIMATVLPIDKLIGKIYPLFGAALLIMAVGVAGGLIIKGYNIPNINLQNM
                     NPNGTPLFPYLFITIACGAISGFHATQSPLMARCVEKESEARPVFYGSMVAEGIIALV
                     WAAAAMSYFHGIPQLNVIFNDGGAATVVNTVSVGLMGPIGGALAILGVVACPITSGDT
                     AFRSARLTIADAMNYNQDAVKNRFLIALPLFAVGVALTFIDFNIIWRYFSWANQTLAM
                     IMLWTGSAYLVKANKNHYITTLPALFMTVVTFSYIMQAKEGFRLPVNISNGIGVIVAI
                     ILGLLFFKKAKHIKEQNNHKLAS"
     gene            4523..5005
                     /locus_tag="L3M60_002734"
     CDS             4523..5005
                     /locus_tag="L3M60_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBN6227377.1"
                     /translation="MDTKKYKDKLLKERESLTNLVEDMKDNTLFGDTTKHTSEKYSSG
                     ELSSYDNHIGDMGTDLYMQNMQNSLINHEEGRLYQIDLALSKIENGTYGTCDLCHNQI
                     DLERLDILPDTSLCNDCAKKENNLLGDAMQNPDVEDSNFYSEDLTNLTDLNKNGILRD
                     "
     gene            5054..5833
                     /locus_tag="L3M60_002735"
     CDS             5054..5833
                     /locus_tag="L3M60_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBN6227378.1"
                     /translation="MKKSIWGICICLLSIVGVYTYNHNTHNQDDLKEAYSLSENKISD
                     YEDVIIKKGSDDEKVIALTFDDGPDEVFTPQVLDILKKNNVKATFFVVGEKVEYNKEL
                     LKRQYTEGHEIGNHTFTHINVAKNSYDKVEKEITDTQNAVKNVIGVEPKIFRPPYRAM
                     SKSVCDIIVSKKMNIILWSNLDPRDWSNPGVGNIINTILTKVQNGNIILLHDYNNRRN
                     DKSQTIQALEVVIPKLKEKGYKFVTISELIQHLDKNKQVQK"
     gene            5992..6438
                     /gene="lspA"
                     /locus_tag="L3M60_002736"
     CDS             5992..6438
                     /gene="lspA"
                     /locus_tag="L3M60_002736"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBN6227379.1"
                     /translation="MLYILIIILLIGLDQLSKMWVLNNLVDVSTIPIINNVFHLTYVE
                     NRGAAFGLLQNNQWIFIIVALLATVFGLYYLNTRKVHISGKLGIILIISGALGNLIDR
                     VRLGFVVDYFDFRIIWEYVFNVADVFVVVGTVFLCIYVLFFESKSR"
     gene            6407..7354
                     /locus_tag="L3M60_002737"
     CDS             6407..7354
                     /locus_tag="L3M60_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HBN6227380.1"
                     /translation="MFYFLKVKVGDLMYEVKEFLVLEEEEEVRLDIYLAEQLGDMSRS
                     YIQKIIKDGKVKVNNKIEKAKYLVKEEDNIVIEIPEPKVLEVVPQDIPIDIVYEDDDV
                     LIINKPQDMVVHPAPGNYENTLVNGILYHCKDKLSSINGVIRPGIVHRIDKDTSGLLM
                     IAKNNYAHNFLAEQLKEHTITREYEFICYGVVKENKITVDKPIGRNPKDRLKMAVVKD
                     GKNAITHFEVVERFDKFTHMRAKLETGRTHQIRVHALSINHPLLGDYVYGPKETKFKL
                     KGQTLHAKKLGFIHPTTKEYIEFNSELPEYFKEVIKKIK"
     gene            7713..8246
                     /gene="pyrR"
                     /locus_tag="L3M60_002738"
     CDS             7713..8246
                     /gene="pyrR"
                     /locus_tag="L3M60_002738"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001156491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HBN6227381.1"
                     /translation="MVEKAQLMDEKAIARAITRISHEIIERNKGVENLVLVGIKTRGV
                     PIANRISKKIEQIEGTKVDTGDIDVTLYRDDLEKIDVEPVVKGTYLDFNVNDKTVVLV
                     DDVLYTGRTVRASLDAIIDIGRPKSIQLAVLVDRGHRELPIRADYVGKNVPTSRHEII
                     SVSLLEIDGEDSVTIKE"
     gene            8529..9737
                     /locus_tag="L3M60_002739"
     CDS             8529..9737
                     /locus_tag="L3M60_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBN6227382.1"
                     /translation="MKKTIMSLQHLLAMFGATVLVPILTGFNPSVAIFCAGVGTLIFH
                     FCTEGKVPAFLGSSFAFIPVILAAKEAYGGDLAYAQGGIIVAGLIYIIMSIIVKIVGV
                     NKIKLYFPAQVTGAMIVVIGLNLLPTAFNMASANFVIAFITLAIAILTNKFGRGFIKQ
                     LGILIAVFSGYIICLILGLVDITTITEASLFAIPNFTVPKFSLGAIVIISPVVLAVFM
                     EHIGDMTTNGAVVGKNFIENPGLNRTLLGDGFATIVAGCLGGPANTTYGENTAVLAIT
                     KNYDPSILRRTAIFAILLACVGKFGGFLQSIPGSVMGGISIMLFSMITYVGLKTIRDS
                     SCVESKINILIIAFILLIGLGTTYLSNKGISIGIPITSTVKITGLSLAAIVGIVLNRI
                     LNNQDFKVEE"
     gene            10025..>11552
                     /gene="abc-f"
                     /locus_tag="L3M60_002740"
     CDS             10025..>11552
                     /gene="abc-f"
                     /locus_tag="L3M60_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HBN6227383.1"
                     /translation="MLLVKVENLKKYYADKLILDIDKLEILENDKIGLVGSNGQGKTT
                     LLKAILGEIEIDEGYIYLTESYSYISQSENNIEICSDSKEKSLLNAPDRFEEYLSGGE
                     KVKLKIADALSNKKNIIIADEPTSNLDKKSIGVLEEMFKRHEGALLLISHDRRFLDEL
                     CTTILELEDGKLKAYKGNYTDYLMQKDEEVKRAYFEYQEYVKEKKRLEKALLHKKSLS
                     DGIRKTPKRMGNSEARLHKMGGQTNKKKLDSNVKAIKSRIDKLEVKNKPKASIEMKIK
                     IQNGMEIISKNLVEIKDMTLKLGNKLLLDNVSFKIKRGKKIALLGDNGCGKSTMIKEI
                     LNNKNDNIKINNKVKVGYFDQHQSLLDEAKSVLYNTKVNSSFDESFIRTNLNLFGFKG
                     DDVYKKVKVLSGGEKVKIALCKIILEDNNFLIFDEPTNYLDIKSMEALEKALINTDKT
                     MLIVSHDRVFVSHICNYIIEIKDTKIREFDCNYDEYIINRNKKNPSRESKIKKENLLV
                     L"
BASE COUNT         4593 a         1100 c         2035 g         3824 t
ORIGIN      
        1 aaattaaatc aatatattat tataaaaaat ttacttatat taaataatat gttttatatt
       61 aacttagata ataaaaattt tctggaattt ttattattat acgtgataaa aatagttatg
      121 atcatataat tcagaaaata aaatttaggg gtatcccttt tggaagaatt atttaacaat
      181 aattatggaa gtaactttca aggttggcaa attattatgg taaatataca ttataagcat
      241 tataatgatt tattgttaaa catattattt taaatatagg atttatttac tttatccata
      301 tacggctata taataaatat ataacacaga gatgataatg tgtgtttagc acaattaaat
      361 caaatgagat atttgaacaa agaatagtat aaaattttta aagaaatatg ttgtatgagt
      421 aataatctac ataacattgt attatataat attcgaaagt attacttcta agaaaagaga
      481 tttctaaagt ataaaattaa atactagaat tgtaaagtta atgataacta caagttatta
      541 caagcttgtg ttagtcagtt agtattagtt gttaatagaa gtatatccat ttttttattt
      601 aattaaaagg ttaaatgtgg aaaatgtcga gataggatta cttcaaatat acaatttaca
      661 acaaacataa taaatttaga gtgtgtaaag atttattttt taaaggggaa atatgaaagc
      721 atcaatttta atcacattaa tagaaaagac aagcctcatt gtagtactat ttcttctaat
      781 aacaaagtta aaaatattta agcagatatt tcaaaaagaa gaatatagct ttaatgattt
      841 ggtctgtata gcattagttt ttactttttt agccatattt ggaacttaca gtggtataaa
      901 ttatatgggg tcaatagtaa acactagaat aatatctata gtatcaggag gcattttatt
      961 tggtccaatg gtaggtatta cagcaggagt tttttcgggg attcatagat attttatgga
     1021 tattggaggg ataacatctg ttccgtgtct tttatcaagt atactagcag gtgtattatc
     1081 aggatttcta tataagagga taccaaagca acatagagta atgtatggta tattggtagg
     1141 catgatttct gaaagtttta ctatactact tatatactta atatcatatc cacattcttt
     1201 agcaattcaa ataattggtg gaatatattt acctttaata gttgggcaga ttggaatagg
     1261 atttgttata tctatagtag aggggattga gaaagataaa aaggatatag aagcgagaaa
     1321 taaagcagaa ataaaagcac ttcaaagaca aattaatcca cattttttgt ttaattccct
     1381 gaacacaata gcttcattta taagatttag tccagataag gctagagagt taataataaa
     1441 tttatcaact tatctaagat ataatcttga atatagtgat aatttaattg atataaataa
     1501 agagattgaa caggttaaat cttttgtaga gatagaaaag gctagatttg gagagttgct
     1561 aacagtatct tatgatatag aagatgtaaa tataaagatt ccaagtttaa ttatacagcc
     1621 actagtggaa aatgcaataa tacatggtat tttagaaagt ggtagagctg gtgcagtgaa
     1681 aatatccata aaaaagttgc catattcatt agaaaatact gtgagaatat caatagagga
     1741 taatggtata ggcataagtg aggaaattat aaataatgta tatcaagaca atatgccaga
     1801 aaataaaatt gggctttata atgtacattt aaggcttaaa ctgatgtatg gaagagggct
     1861 taatataaga agaatagata atgggacttt gatagtattt tatgtaaagg agtagaagta
     1921 tgagagctat aatagtggaa gacgagtttc cagcaagaaa ggaattgaga tattttattg
     1981 aaaataaaag tagtatcgag gtagtaagtg aatttaccaa tggtatagag gtattagact
     2041 ttattcagga aaataaaata gatgtaattt ttttagatat aaatatacct catcttgatg
     2101 gaatgctact tgcaaaaaca ttaaatcagt ttaaatcaag acctaaaatt gtttttataa
     2161 cagcatatga aaattatgca gtagatgctt tttctttgga cgtatttgat tatatattga
     2221 agccatattc tgaagagcga attatttcta tgctgaataa attagaaaaa agtgagagga
     2281 gtgatattga actgtctagt ggtaatagta atttatacaa atataaaaaa gaaacagtaa
     2341 atcaggatat agaagaaata acacataaaa ttagcctttg gaaaggtgat aaattggttg
     2401 taatagatat tgatgatata tattattgtg aagctaatga aaggcagaca ttcatatata
     2461 ctgagaaaga aaagtttata ttaaaagaag gcatatcaga agtcgaaaat cttataaatg
     2521 ataaaacgtt ttttagaaca cataggtctt atatcgtaaa cttaacaaaa gtaaaagaaa
     2581 ttataccctg gtttaataat acatacatat tgaaattaaa aaataatgac tatgaagtaa
     2641 ctgtcagtag aagtaaagta aaagaattta ggttgttaat gcatatataa agtcatttca
     2701 tgtacttaaa tatgcactta atgtaattaa tatttatatt agcataaaaa agtattacta
     2761 tttgaactgg aaatagtttt cttaaactca taaaaattta taaagcaatt atataaaaac
     2821 taaggggatg aatttatggt aagtttttta ggttcaatag tggtacttat agtaggttat
     2881 tttatctatg gtacttttgt agaaaaggtt tttggaatca atgacaaaaa ccaaactcca
     2941 gctatagcct gtagagatgg agtagattat gtgccaatga aatggaagag aatcttttta
     3001 attcagtttc taaatatagc agggttaggt cctatatttg gagctataca aggggcgtta
     3061 tttggaccat cagcattttt atggattgta tttggaacaa tctttgcagg tggagtacac
     3121 gattttgctt caggatattt atcaatgaag aacaaaggta catctgcatc agagttggta
     3181 ggtatttatc taggaaatgg tgcaaaaata gcaatgagaa tatttagtgt tattctttta
     3241 gtattggttg gagttgtatt tgtaacaggt ccagcaggat tgttaaagac attgacaggt
     3301 gtaaacgcac aaatatgggt tggagtaata atactttatt atattatggc tactgtttta
     3361 ccaatagata aattgattgg aaaaatatat cctttatttg gagcagcatt attaattatg
     3421 gcagtaggtg tagctggagg attaataata aagggatata acataccaaa tataaattta
     3481 caaaatatga atccaaatgg aacaccttta ttcccatatt tatttataac aatagcatgt
     3541 ggagcaatat caggttttca tgcaacacaa tcaccactta tggcgagatg tgttgaaaaa
     3601 gagagtgagg caagaccagt attttatggg tcaatggttg cagaaggtat aatagcttta
     3661 gtttgggcag cagcagccat gtcatatttc catggtatac ctcaacttaa tgtaatattt
     3721 aatgatggtg gtgcagcaac agtagtaaac acagtatctg ttggattaat gggtcctatt
     3781 ggaggagctt tagctatact tggagttgtg gcatgtccta taacttctgg ggatacagca
     3841 tttagaagtg ctaggctaac aatagcagat gcaatgaatt ataatcaaga tgcagtaaaa
     3901 aatagatttt taatagcttt accattgttc gcggttggcg ttgctcttac atttatagat
     3961 tttaatataa tttggagata tttctcttgg gcaaatcaaa cacttgcaat gataatgttg
     4021 tggactggtt ctgcatattt agttaaagca aataaaaacc attatataac tacattacca
     4081 gcacttttta tgactgtagt aacatttagt tatataatgc aagcaaaaga aggatttaga
     4141 cttccagtta atatatctaa tggaataggt gttattgtag caataattct tggtctttta
     4201 ttctttaaaa aagcgaaaca tattaaggaa caaaataatc ataaattggc aagttagtaa
     4261 aataataact ataaaaatta aaaatttata tttaaactat gttcaaagaa gaggctgtct
     4321 caagatagag ataagtctct ttttcattgt gaaattataa aaaatatatt tgactttata
     4381 caaaaaagag gatttaatga actaaaaaat ttattttgag acattccatt ttttgaatca
     4441 aatcttattt ttattgtatg tttaatttac ttttccacat actattttta tgatataaat
     4501 attaaaatta gaggtgttaa aaatggatac taaaaagtat aaagataaat tattgaaaga
     4561 aagagaaagt ttaactaatt tagtagagga tatgaaagat aatactttat ttggagatac
     4621 gactaaacat acaagcgaaa agtattcatc aggagaatta tctagttatg ataatcatat
     4681 aggagacatg ggaacagatt tatatatgca aaatatgcaa aacagtctaa taaatcatga
     4741 agagggaaga ttatatcaaa tagatttagc attatctaaa atagagaatg gaacatatgg
     4801 gacttgtgac ttatgtcata atcaaataga tttagaaaga ttggatatat tgccagatac
     4861 aagtttatgt aatgattgtg caaagaaaga aaataattta ttaggtgatg caatgcaaaa
     4921 tcctgatgta gaagattcta atttttattc agaagaccta acaaatttaa ctgatttaaa
     4981 taagaatgga atattaagag attagaaaac tgaatcatat attgttagaa tgaattagaa
     5041 aagaggtaat gacatgaaga aatctatatg gggtatatgt atatgtttat tatctatagt
     5101 aggtgtatat acatataatc ataatacaca taatcaagat gacttaaaag aagcttatag
     5161 tttgagtgaa aataaaatta gtgattatga agatgtaata attaaaaagg gaagtgatga
     5221 tgaaaaggtc atagctctaa cgtttgatga tggtccagat gaggtattca caccacaagt
     5281 acttgatata ctaaaaaaga ataatgtcaa agcaactttt tttgtagttg gagaaaaagt
     5341 agaatataac aaagaattat taaaaagaca gtatactgaa ggacatgaga taggaaatca
     5401 tacatttaca catataaatg ttgcaaagaa tagctatgat aaggtagaaa aagaaataac
     5461 agatacacaa aatgctgtaa agaatgtaat cggagtagaa ccaaaaatat ttagacctcc
     5521 atatagagca atgagtaaga gtgtttgtga tattatagtt tctaaaaaga tgaatataat
     5581 tctctggtca aacctagacc ctagagattg gtctaatcct ggagtaggca atattataaa
     5641 tactattctt acaaaagttc aaaatggtaa tataatactt ttacatgatt ataataatag
     5701 aagaaatgat aagtcacaaa ctatacaagc tttagaagta gtaataccta agctaaaaga
     5761 aaaagggtat aagtttgtaa caatatcaga gcttatacaa catttagata aaaataaaca
     5821 agtacaaaaa taaaaaataa ttattaaaat attaatgatg cttgtatttt aaatttataa
     5881 gcatcattaa tattttaaga tagttgctct ttagagcatt ttttttgtat aataataata
     5941 gtgtgtttca aataaaattg ttttttaaat aatttatagg gggaaaaatc attgctatat
     6001 atattaataa taattctact cataggctta gaccaactgt ctaaaatgtg ggtattgaat
     6061 aatttggtag atgtatcaac aataccaata ataaataatg tatttcattt aacttatgtg
     6121 gaaaatagag gtgcagcatt tggattatta caaaataatc aatggatatt tataattgtt
     6181 gcattacttg caacagtatt tgggttatac tatcttaata caagaaaagt acatatatct
     6241 ggaaaattgg gaattatatt aattatatct ggtgcattgg gaaatctaat tgatagagta
     6301 cgattaggtt ttgtagtaga ttacttcgac tttagaatta tatgggaata tgtattcaat
     6361 gtagctgatg tatttgtagt tgtaggaact gtgtttttat gtatatatgt tttatttttt
     6421 gaaagtaaaa gtaggtgatt taatgtatga agtaaaagag tttttagtac ttgaagagga
     6481 agaagaagtt agacttgata tttatctagc agaacaatta ggtgatatgt caagaagtta
     6541 tatacaaaaa attataaaag atggaaaagt aaaggttaat aataaaatag aaaaggctaa
     6601 atatctagtt aaagaggaag ataatattgt tatagagata ccagaaccta aagtattaga
     6661 agttgtacct caagatattc caatagacat tgtatatgaa gatgatgatg ttttaataat
     6721 aaataaacct caggatatgg ttgtacatcc tgctcctgga aactatgaaa atacgctggt
     6781 aaatgggatt ttatatcatt gtaaagataa gttgtcatca ataaatggag ttattagacc
     6841 tggtatagtt catagaatag acaaagatac ttctggtctt cttatgatag ctaagaataa
     6901 ttatgcacat aattttctag cagaacaatt aaaagaacat actataacta gagagtatga
     6961 atttatatgc tatggtgtgg ttaaagaaaa taaaataact gtggataaac ctatagggag
     7021 aaatcctaag gatagattaa aaatggctgt agtaaaagat ggaaaaaatg caatcactca
     7081 ttttgaagtt gtagaaagat ttgataaatt tacacatatg agagctaagc ttgaaactgg
     7141 tagaactcat caaataagag ttcatgcttt gtctataaat cacccacttt taggtgatta
     7201 tgtatatggt ccaaaagaaa ctaaatttaa attaaaagga caaactttgc atgctaagaa
     7261 gttgggattt atacatccta ctactaagga atatatcgaa tttaattcag aattacctga
     7321 gtattttaag gaagttatta aaaagataaa ataatcattt gtataatact tataaatcat
     7381 tttttgtttt tatgtaagta aataaattta gtgaaattta aataaaatat cttatattaa
     7441 agtattttgt ttaaaaaagc ttgaaaaatt tgaaatataa atatataatt tgaattgata
     7501 gattaaaaaa ttatctttaa acgatacaga gatatcgtaa gacaattgct aaattacact
     7561 gtatattttt tatataagag cttcttgtct tgtgatgaga agttttttgt tgtttaaatt
     7621 gaaattttaa gtaaaaataa atttataaaa atttaaaaga aaggagtaga tttgtcaaat
     7681 agatatatta tatctttgac aataattatg atatggtaga aaaagctcaa ttaatggatg
     7741 aaaaggcaat agcaagagca attactagaa taagtcatga aataattgaa agaaataaag
     7801 gtgtagaaaa tctagtttta gttggaataa aaactagagg tgtaccaatt gcaaatagaa
     7861 tttctaagaa aatagaacag atagaaggaa caaaagtaga cacaggagat attgatgtga
     7921 ctttgtatag agatgatttg gaaaaaattg atgtcgaacc tgtagttaaa ggaacttatt
     7981 tagattttaa tgtaaatgat aagacagtag ttttagttga tgatgtttta tatactggaa
     8041 gaactgttag agcttcacta gatgctatta tagatatagg aagacctaaa tcaatacaat
     8101 tagcagtgct agtggacaga gggcatagag agttgcctat aagagcagat tatgtaggaa
     8161 aaaatgtacc tacttctaga catgaaatta tatctgttag tttattagaa attgatggag
     8221 aagattcagt tacaatcaaa gaataataat ttttaagtat tatataaaag cttagcattg
     8281 aatagtcagg aaggaggata aagcttacat agattgcaca aaataaatat tataatttta
     8341 tggatgtggg tcttaatgta gtctttattc tcctttattt gagttttgat gtcgtaaaat
     8401 gtaaaataat aataaattat ataataagta tatagaggtt aaaaaataga atctagggaa
     8461 tgataattaa ttaaaagata ttaattaaac agaggattta tttatatata ataattttgg
     8521 aggtttttat gaaaaaaacg ataatgtctt tacaacatct attagccatg tttggagcga
     8581 cagtattagt tcccattttg acaggtttta atccgtcagt tgcaattttc tgtgctggtg
     8641 ttggtacatt gatatttcat ttttgtacag aaggaaaagt acctgccttt ttaggttcaa
     8701 gttttgcgtt tataccagtt atattagcag ctaaagaggc gtatggtgga gatttagcgt
     8761 atgctcaagg tggtataata gttgcaggat taatttatat tataatgtcg attatagtaa
     8821 aaatagttgg agtaaataaa ataaaattat attttcctgc acaagttaca ggagctatga
     8881 tagtagttat aggattaaat ttattgccaa cagcttttaa tatggcttca gccaattttg
     8941 taatagcatt cataacttta gcaatagcaa ttttaaccaa taagtttggt agaggattta
     9001 taaaacaatt aggaatatta atagccgttt tttctggata tataatctgt ttgatattag
     9061 gattagtaga tataactaca attacagagg caagtttatt tgcaatacct aactttacag
     9121 ttcctaagtt tagtttagga gcaattgtga ttatatctcc agttgtgtta gctgtattta
     9181 tggaacatat aggggatatg actactaatg gtgctgtagt aggtaaaaat tttatagaaa
     9241 atcccggatt aaatagaact ttacttgggg atggatttgc aactatagtt gcaggttgtt
     9301 taggaggtcc agccaataca acttatggtg aaaatacagc agtacttgcc ataactaaaa
     9361 attatgaccc ttcaatactt agacgtacag caatatttgc catattgctt gcatgtgtag
     9421 gaaagtttgg tggatttctt caaagtatac caggttcagt tatgggtgga attagtataa
     9481 tgctattttc aatgataact tatgtaggat taaaaactat aagagatagt tcttgcgtag
     9541 aaagtaagat aaatatattg ataatagcat ttatattatt aataggtctt ggaacaacat
     9601 atttgtctaa taaaggtatt tctattggaa tccctataac aagtacagta aagataactg
     9661 gccttagtct agctgcaatt gttggaattg tattaaacag aattttaaat aatcaggatt
     9721 ttaaagttga agaataataa attgtatatt gaataattta aattagttaa ttttctatag
     9781 acatttgtaa ttaatgttga tataatgatt aaaaataaat gaaagaaagg gcaaggtaac
     9841 tgggacaatg agaatataat ctatttttta taattaaagt attgtaattt ttgaaaagcc
     9901 tattttggtg gctttatttg cgatgcttaa attaaggaga gtagattata gtctatacgt
     9961 ttatttgtta tgcctattta tagaatctct ccttatattt aagagtgtta aggagggatt
    10021 ttttatgtta ttagtaaaag tagagaactt aaaaaaatat tatgcagaca aattaatttt
    10081 agatatagat aaacttgaaa tattagaaaa cgacaaaata gggctagttg gttcaaatgg
    10141 tcaaggaaaa acaacattat taaaagcaat attgggtgaa atagaaattg atgaaggata
    10201 tatttacctt acagaaagtt attcctatat aagtcagagt gaaaataata ttgaaatatg
    10261 tagtgatagt aaagagaaga gtcttttaaa tgctccagat agatttgaag agtacttatc
    10321 aggaggggaa aaagttaagt taaaaatagc agatgcactg agcaataaaa aaaatatcat
    10381 aatagctgat gagccgactt caaacttaga taaaaaaagt attggagttt tggaagagat
    10441 gtttaaaagg catgagggag cattattatt aatatctcat gatagacgtt ttctggatga
    10501 gttgtgtaca actatattag aactagaaga tggaaaacta aaagcctaca aaggcaatta
    10561 cactgattat ttaatgcaaa aagatgaaga agtaaaaaga gcttattttg agtatcagga
    10621 atatgttaaa gagaaaaaaa ggcttgaaaa agctctttta cataaaaaat ctttaagtga
    10681 tggcataaga aaaacaccaa aaagaatggg taattcagaa gctagattgc acaaaatggg
    10741 gggtcagact aataagaaaa agttggactc aaatgtgaag gctataaaaa gtagaattga
    10801 taaacttgag gtaaaaaata aacctaaagc ttctatagaa atgaagatta agattcaaaa
    10861 tggtatggag ataattagca aaaatctagt agagataaag gatatgactc taaaattagg
    10921 aaataaactg ctattagata atgtttcttt taagataaaa agaggtaaaa agatagcatt
    10981 attaggtgat aatgggtgtg gaaaaagtac tatgataaaa gaaatattga ataataaaaa
    11041 tgacaatata aaaataaata acaaagtaaa ggtaggttat tttgaccaac atcaaagctt
    11101 attagatgag gcaaagagtg ttttatacaa tactaaagtt aatagttcat ttgatgaatc
    11161 ttttataagg acaaacttaa acctgtttgg atttaaagga gatgatgtct acaaaaaagt
    11221 aaaagtactt agtggtggag aaaaggtaaa aatagcacta tgtaaaataa tattggaaga
    11281 taataatttt ttaatatttg atgaaccaac aaattactta gatataaaat ctatggaagc
    11341 actagaaaag gcactgataa atactgataa aactatgctt atagtatctc atgatagagt
    11401 atttgtatca catatttgca actatattat agagataaaa gacactaaaa taagggagtt
    11461 tgattgtaat tatgatgaat atataattaa tagaaacaaa aaaaatccta gtagagaaag
    11521 taagattaaa aaagaaaatc ttctagtatt ag
//
LOCUS       DAFNNB010000124        11409 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0125, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000124 DAFNNB010000000
VERSION     DAFNNB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11409
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0125"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            249..1517
                     /locus_tag="L3M60_002741"
     CDS             249..1517
                     /locus_tag="L3M60_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HBN6227384.1"
                     /translation="MEASIHTIASNNLLIIFAIVSITGIICSKLSEIIKVPDVVLYLL
                     VGILIGPSFLKFIDIRGFQIENQLILTFGSAFILYLGGKEISLKVLRNVKISVFLLAT
                     LGVVISAFIMQQVIGFTFGISAMTALLAGTIIASTDPATLVPIFNQVKIKDRVKQTVI
                     SESAFNDATGAILTSAVLAVILSNKFSLGENIYELGTMVIVGVLVGLITGILLLKLVN
                     DKPYGIFKDFAPIISVISVVVAYELSTKLGGSGYMSCFIVGIITGNKKNFKIWLSQKS
                     YDADFYVAETLGTICRMAIFIILGSQVNLAQLSKYFMPSVITVLVLMFIARPLCVLVC
                     TLVDREAKWSKSEILFMMWVRETGVIPAALCGIISAMKVPGYEVISSVVFMTILITLV
                     IQGSTTKLVAKKLGLLEEEVINISEKVSTF"
     gene            1711..2499
                     /locus_tag="L3M60_002742"
     CDS             1711..2499
                     /locus_tag="L3M60_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HBN6227385.1"
                     /translation="MFTVMDIVQPDTVEEAYSILNKRKTNQVIGGSAFLRMGKKRIGT
                     GIELSNLNLDYIKEDEDCVEIGSMTTFRTLETSSIIKNNFGRIIEDSVKNIIGVQFRN
                     VVTVGATVFSKYGFSDLIVALLSLDTEVELYNIGRISLEEFLNRDYEKDLLIKIYIKK
                     TNKNASYKSLRNAKSDYPILNVSVSKHMKQFKLCVGARPQKATIAKQASEFLSNNEIN
                     EINIDKAVEIASEELTFGSNMRASRDYRKAMSKVLLKRAIMEVI"
     gene            2499..2948
                     /locus_tag="L3M60_002743"
     CDS             2499..2948
                     /locus_tag="L3M60_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HBN6227386.1"
                     /translation="MLVKININGKKKSVDIEPEEYLVDTLRKAGNLSVKRGCDTGCCG
                     LCTVLIDKKPTLSCATLTLRVLDREITTIEGLENEVKEFSEVLVKEGAEQCGFCSPGF
                     ILTVIAMKDELENPTDEEIKHYLTGNLCRCTGYMGQLRAIKTYLEVK"
     gene            2952..5216
                     /locus_tag="L3M60_002744"
     CDS             2952..5216
                     /locus_tag="L3M60_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBN6227387.1"
                     /translation="MNIVGKGIKKIDGMSITTGKPVYTDDLSAKDALVVKILRSPHAY
                     AKIKDIDTSKAEMVDGVECVLTYKDVPKSRFTLAGQTYPEPSPYDRLILEDVVRYVGD
                     EVAIVAAVDEKTAIKAMNLIKVKYEVLTPVLDFEEAIGNNTVVHDEDRHCNFDIGMQR
                     EKNIVSHHDYTKGDVEKVLSNCDIVIEETYYTQAQAHAMMETYRAYNYLDHTGRLVVV
                     SSTQIPFHVKRHLSRALELPSSKIRVIKPRIGGGFGGKQTACVEIFSAIVTLKTGKPA
                     KIIYDRKETFNCSTSRHAMRLKVRLGATNDGIIKAIDIKALSDTGAYGEHASTTIGLV
                     GEKTLPMYNKVEATRFIADVVYTNKMPAGALRGYGASQGCFAVESTINMLANKLKIDP
                     TDLRLKNVVTEGETTFAYGKTLNSVDLIKCINKGKELIGWNEKYPCKYLDNGKVRSVG
                     MAITMQGSGIQGLDKSSAEIKLNDCGDYTLMIGSTDMGTGSDTILAQMAAEVLETSIE
                     NITVISADTDLVPYDPGSYASSTTYVTGMAVVKAAQDLREKIISAGANKLGVDKDEVE
                     FDGIKLTSKKGSTLLFDIAVDSTIGPDTNQLIGLASHGSPVSPPPFIAGFAETEIDKE
                     TGKVDLVNYVAVVDCGTVINKNLAKIQVEGGIVQGIGMALFEEVRYTEKGHMDTNTFM
                     QYKIPARCDIGNVKVDFVETYEPTGPFGAKSVGEVVINTPLPAIQEAVFNGIGVRINT
                     LPITPEKVFMAMNK"
     gene            5356..5664
                     /locus_tag="L3M60_002745"
     CDS             5356..5664
                     /locus_tag="L3M60_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227388.1"
                     /translation="MAGNLNNMRAVNNFRGDKNILECLVSFEGRSISQRKAKVFFKEK
                     QNQIEIDFVEEEISKLIENVVLNTSYQEMLYDEIEKQLEIDCIGTWMILSKLKDGRVH
                     "
     gene            5950..6450
                     /locus_tag="L3M60_002746"
     CDS             5950..6450
                     /locus_tag="L3M60_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HBN6227389.1"
                     /translation="MRKNNINIALTSAITAIYAVLTLSLGFISYGPIQFRVAEIMMLL
                     AFLDKGYIVGLTLGCFLANVIGPYGVPDIIFGTLATFISASMIYITRKLGKQNKSTLI
                     IASLWPTIINSIVVGLMLNIFFGLPLILSMIQVGFGEFVVVTIVGVPFFCFLMKKYKN
                     IINIKF"
     gene            6503..7489
                     /locus_tag="L3M60_002747"
     CDS             6503..7489
                     /locus_tag="L3M60_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HBN6227390.1"
                     /translation="MSKIKRLTDVWTYINDKQKFTIKELSEEFNLSAKTIQRYLKELN
                     KMGLPIQAEQGRNGGYRVLNNSYIPPVIFTEKEVMAMLFALKSLQVYNYKSIEIEINS
                     IMRKVSYERKSSIKVGIENIKRYIGFVANNSNDGEFKSINVTEFFRASSESLILNIKY
                     KFGNQILEKKICPIGIYLNKGAWFSPAYDCKIDKIILVHIDNVIDIKRTGESKKIHIN
                     LEEWLDEVYNNLYENRSTTKKIGETIYLNVLLTKEGVSKIKDSSYNLDMELNEDGSGT
                     ISTFIRVEDVDYYASILFLIGNNAKVIEPKFLNKLLYDKANELRYFYEENMV"
     gene            7539..8261
                     /locus_tag="L3M60_002748"
     CDS             7539..8261
                     /locus_tag="L3M60_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein"
                     /protein_id="HBN6227391.1"
                     /translation="MENFNKNAKIIGIGAEGINIINEIEEKIKANMDIEKININQEIE
                     KEYVRSLLDGVDILFLIYSSEDKHIKEIIKAISYMSNERRVLSIGMDCSEKENKEALE
                     LGREFKINNDSIFKFVNLMNIMVESISDSCMINIDITDLKEAIVGDKGIKYSFEEFED
                     TKEYSEMADILFDRMEYIGEEFISKKGIVFVEGSSEFSIMELNDLINNIQRKIEESYE
                     VIFSLYMKENLDGNIRIGLLYN"
     gene            8290..10224
                     /locus_tag="L3M60_002749"
     CDS             8290..10224
                     /locus_tag="L3M60_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6227392.1"
                     /translation="MSENIRVTEYWLMEEFFYQETDTPQIFREESIIALGFDIKINLK
                     KVFGMGKKSEKYINENCNDKDFIKRFVSIKKGDYFLYKAYQNIEEKVKCNVSIGMVEE
                     DFENGYELSHILGHTLPVKWMYNFDTVSNCKVYTSEFYKLNNNDISSYLEIINSQDKK
                     SLRKRIEESENNKWYLKNYYYKNVDKCVNDEELFIQPTNLEQLENIKNIKTDDYILLY
                     NSEKVICENDHTMKSLFGVCQVMEGFDKEKVSTLEDGYFFPVRWIEYEKENIDGNAWV
                     IFENNSMVEKYVKRFTNKIPLNTILYGPPGTGKTYNTKQMVYNLVNKTSLRNRYYTGY
                     LDYKNNVEFCTFHQSYGYEEFIEGLKSDGEGNFKPEDGILKDISIEACYDALKFEKKM
                     SFELEKDSLTLEAIKKRKKKLVLENIEYSDSFNFEESENYVLIIDEINRGNVSKIFGE
                     LITLLEDDKRLTKSNQTIVKLPYSKEKFFLPPNLYIVGTMNTSDKSIALLDIALRRRF
                     NFEEIMPNYDLLSIIDGIDIRKMLYTINKRIEFLYDRDHVIGQAYLLAVENINDIVVV
                     FRSKIIPLLQEYFYYDSEKVGLILGGIGRNEKDKYIVYKEELIASELFKNNNASIMES
                     KVNYCIKQNISSEELKNIYE"
     gene            10217..>11409
                     /locus_tag="L3M60_002750"
     CDS             10217..>11409
                     /locus_tag="L3M60_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HBN6227393.1"
                     /translation="MSKNIYVKETYEWIRVGNGENELTEIEYEKLLKYLENNNDVLKS
                     NIIDIKYKKLRFINYVGIICFENIILEILPKLSLSDNLVKDREILLQMLSICNKIPII
                     MNEKIRLSLKNYNLLNFFIMYFIESMQIQMKKGIYFEYINKIENLNVIRGKILLSTYA
                     KEKCISPMKIRCEYDEYSENNFLNQVLKKACISILCRINDNSIQGKIKKILNYFQNVD
                     LIYIDRKKLLDYKFCKNNDRFRDCYLLARLLLLNLSMDNSQDNEEAFSILFEINTLYE
                     EYIGILIKSIWDNSFRETYIQDKSKFLLKNEQTGKKNFNLRPDIVLYDLKSEYEIIID
                     TKWKAIEVDSNVFYRSSDIYQMYAYITTYENAKRCILLYPCIQKDKNYSSWKLSESFK
                     GKFIEA"
BASE COUNT         4591 a         1030 c         2036 g         3752 t
ORIGIN      
        1 aaaatatcta aaaagatatg gatataaaaa atattaatat atatagacta taaacttgac
       61 aaaatcaacc ttttaatgaa tagcaaaaaa tggaaaaatt atataagcgt tatttaatta
      121 tcaaaaatga aagctaaatt gcatgtgcta aaaaaaatga ttgataacat ttatttgtgt
      181 gctataatta tatagtaaaa cgaataagta tattgcataa tgtatgcaaa ttgggaggag
      241 agacagacat ggaagcatct atacacacaa ttgccagcaa taatttattg ataatttttg
      301 ctattgtaag tataactgga ataatttgta gtaaacttag tgaaataata aaagttccag
      361 atgtagtgtt atatttactt gtaggtattt taataggacc atcattttta aaatttatag
      421 atattcgtgg atttcaaata gaaaatcaac taatacttac ttttggttct gcatttattt
      481 tatatttagg tggaaaagaa ataagtttaa aagtattgag aaatgtgaag atatctgtat
      541 tcttattagc tactttagga gttgtgatat ctgcttttat aatgcagcaa gtgataggat
      601 ttacttttgg gataagtgca atgactgctt tattagcagg aactataata gcatcaacag
      661 acccagctac tcttgtacct atatttaatc aggtaaagat aaaggataga gtaaagcaaa
      721 ctgtaataag tgaatcagct tttaatgatg caacaggagc tatactaaca tcagcagtgt
      781 tggctgtaat tttgtcaaat aaattttcct taggtgaaaa tatttatgaa ttggggacaa
      841 tggttattgt cggagtttta gttggtttaa ttacaggaat actgcttttg aaattagtta
      901 atgataaacc ttatggaata tttaaagatt ttgcaccaat aatatctgtt atttcagttg
      961 tagttgcata tgaattatct acaaaattag gtggaagtgg atatatgtct tgttttattg
     1021 taggtataat aactggtaat aagaaaaact ttaaaatttg gttaagtcaa aaatcttatg
     1081 atgcagattt ttatgttgct gaaactttag gtactatttg tcgtatggca atatttataa
     1141 tactgggtag ccaagttaat ttagctcagt taagtaaata ttttatgccc tctgtaatta
     1201 cggtattagt attaatgttt atagcaagac cactttgcgt tttagtttgt acacttgtag
     1261 atagagaagc aaaatggagt aaaagtgaaa tattatttat gatgtgggta agggaaacag
     1321 gcgtaatacc agcagcactt tgtggtatta tttcagcaat gaaagttcct ggatacgaag
     1381 taatttcatc tgtagtattt atgaccatac taattacctt agtaatacaa ggaagtacta
     1441 caaaattagt agctaaaaaa cttggattat tagaagaaga agttataaat ataagtgaaa
     1501 aagtatcaac tttttaaagt tttgatttaa ataataccta ttaatggcta ttttaatatt
     1561 gtctaatgat gattattgaa atagtcattt ttttatgtat aagcaattta cagaatttac
     1621 atattagaca tatttattat caaaatttgt tgaagctatg atataattag ttgtatttaa
     1681 aataagaata gcaaataagg gaggaaattt atgtttacgg ttatggatat tgtacagcca
     1741 gatacagtag aagaagcgta ctcaatttta aataaaagaa aaactaacca agtaattggt
     1801 ggaagtgctt ttttaagaat ggggaaaaaa agaataggaa caggaataga attatctaac
     1861 ttaaatttag attatataaa agaagatgaa gattgtgttg agattggttc tatgactact
     1921 tttagaacat tagaaacaag ttccataata aaaaataatt ttggaagaat aatagaagat
     1981 tcagtaaaaa atataatagg agttcagttt agaaatgtag taacagttgg tgcaacagtg
     2041 ttttctaaat atggtttttc tgacttgata gtagcattac tttcattaga tacagaagta
     2101 gaattatata atattggaag aattagtctt gaagagtttc taaataggga ttatgagaaa
     2161 gatttactta ttaagatata cataaagaag acaaataaaa atgcttctta taaatcactt
     2221 agaaatgcaa agagtgatta tccaatctta aatgtatctg tatcaaaaca tatgaaacaa
     2281 tttaagttat gtgtaggagc aagacctcag aaagctacga ttgcaaaaca agctagtgaa
     2341 tttctatcta ataatgaaat caatgaaatt aatatagata aagcagtaga aatagcatca
     2401 gaagaattaa cttttggttc taacatgaga gcatctaggg attatagaaa agctatgagt
     2461 aaagtacttt taaaaagagc tataatggag gttatttaat gttagtgaag attaatataa
     2521 atgggaagaa aaaaagtgtt gatatagaac cagaagaata tctggttgat actttaagaa
     2581 aagctggtaa tttaagtgta aaaagaggtt gtgacacagg ctgttgtggt ctctgtacag
     2641 ttttaattga caagaaacct acattatctt gtgcaacact gacattgaga gttttagata
     2701 gagaaataac tacaattgaa ggtttagaaa atgaagttaa agaatttagt gaagttttgg
     2761 taaaagaggg agctgagcaa tgtggtttct gttctcctgg ttttatactt actgtaattg
     2821 caatgaagga tgaacttgaa aatcctacag atgaagaaat aaagcattat cttacaggaa
     2881 atctatgcag atgcacaggg tatatgggac agttaagagc tatcaaaact tatctggagg
     2941 taaaatagag tatgaatata gttggaaaag gaatcaagaa aatagatgga atgagtatca
     3001 caacaggaaa gcctgtatat acagatgatt tatcagcaaa agatgctttg gtagtaaaga
     3061 tacttagaag tcctcacgct tatgcaaaaa taaaagatat agatacatca aaggcagaaa
     3121 tggttgatgg tgttgaatgt gttttaacat ataaagatgt accaaaatct agatttacac
     3181 ttgctggtca gacatatcca gagccatctc catatgacag attaattctt gaagatgttg
     3241 tgagatatgt aggtgatgaa gtagctatag tggcagctgt agatgaaaaa actgcaatta
     3301 aagcaatgaa tcttattaaa gtaaagtatg aagtactcac acctgttttg gattttgaag
     3361 aggcaatagg gaataacact gtagtacatg atgaagatag acattgtaat tttgatatag
     3421 gtatgcaaag agaaaaaaat atagtatcac atcatgatta tacaaaaggt gatgtggaaa
     3481 aagtattaag taattgtgat atagtgattg aagaaactta ttatactcaa gctcaagcac
     3541 atgcaatgat ggaaacgtat agagcatata attatttaga ccatactgga agattggttg
     3601 tagtaagttc aactcaaata ccatttcatg taaaaagaca tttatctcgt gcactagaac
     3661 taccatctag taaaattagg gttataaaac ctagaattgg tggaggattt ggtggtaaac
     3721 aaactgcatg tgttgagatt ttttctgcaa ttgttacttt aaaaacaggg aaaccagcta
     3781 aaataattta tgatagaaaa gaaactttta attgttcaac aagtagacat gcaatgagat
     3841 taaaagttag actaggtgct acaaatgatg ggataataaa ggcaatagat ataaaagcac
     3901 tttctgatac tggagcatat ggagagcatg cttctacaac tattggtttg gttggagaaa
     3961 aaactttacc aatgtataat aaagttgaag caacaagatt tattgcagat gtagtatata
     4021 ctaataagat gccagcagga gcacttagag ggtatggagc atcacaaggg tgttttgctg
     4081 ttgaatcaac tataaatatg cttgctaata aattaaaaat agacccaaca gatttaaggt
     4141 taaaaaacgt ggtaacagaa ggtgaaacta catttgcata tgggaaaact ttaaatagtg
     4201 tcgacttaat aaaatgtata aataaaggca aagaacttat tggatggaat gaaaaatatc
     4261 cttgcaaata tcttgataat ggaaaagtta gaagtgtagg tatggcaatt actatgcaag
     4321 gttctggtat acaaggtctt gataagtctt cagctgaaat aaaattgaat gactgtggag
     4381 attatactct tatgataggt tctactgata tgggtacagg aagtgataca attttagctc
     4441 aaatggctgc tgaggttctt gagacgagta ttgaaaatat aacagttata tcagctgata
     4501 ctgacttagt tccatatgac ccaggttcat atgcttcaag tactacttat gtaacaggta
     4561 tggcagtagt taaggcagct caagatttaa gagaaaaaat aatttctgct ggagcaaata
     4621 aactaggtgt tgataaggat gaagtagaat ttgatggtat aaaattaact agtaaaaaag
     4681 gtagtacttt attatttgat atagcagtgg attctacaat tggtccagat actaatcaat
     4741 taataggtct tgcttctcat ggtagccctg tatctccacc accttttata gcaggttttg
     4801 cagaaactga aattgacaaa gaaactggta aagttgattt agtaaattat gtagctgtag
     4861 tagattgcgg aacagttata aataaaaatc tagctaagat acaagttgaa ggtggaattg
     4921 ttcaagggat tggaatggca ttatttgaag aagttagata cacagaaaaa ggacacatgg
     4981 atacaaatac atttatgcaa tacaagattc cagctagatg cgatattgga aatgttaaag
     5041 tagattttgt tgaaacttat gaaccaactg ggccatttgg tgctaaatct gttggagaag
     5101 ttgttataaa tacaccttta ccagcaatac aagaagctgt gtttaatgga attggtgtaa
     5161 gaataaatac acttcctatt acacctgaaa aagtttttat ggcaatgaat aaataattat
     5221 ttatgaaatt ttagactagt tttacatgtt aaacaaccag ttatgtcata cctgttgaaa
     5281 gatatttaat aagtagttat tataatattg tgaattttac ttataaaata caaaattaaa
     5341 taaggggatg atgttttggc tggaaatcta aataatatga gagcagtaaa taattttaga
     5401 ggagataaga acattttaga atgtttagtc agctttgagg gtcgttcaat aagtcagaga
     5461 aaagcaaagg tattttttaa agaaaaacaa aatcaaatag aaattgattt tgtggaagaa
     5521 gaaatttcta aattgattga aaatgttgtt ttgaatacat catatcagga aatgttatat
     5581 gatgaaatag aaaaacaact ggaaattgat tgtataggta cttggatgat attatctaaa
     5641 ttgaaagatg gtagagttca ttgattttaa aataaattat tagagcctaa ttttaaggct
     5701 cttttttact gcttttaatt aacaacagaa aatctatatt taatttacat ataattttaa
     5761 tatcatatat tctatatata taaaattgag tttgtatgta ttatattgta taatataaat
     5821 tgaattagtg aaatagttcg ttaaaataat ttgtttgtat aaaatcattg tttaagttta
     5881 tagattttgt ataaaataaa gttatgttat taattttttt agaagtaaaa tttttactag
     5941 gagacagata tgagaaaaaa taatattaat atagcattaa cctcagcaat tacagcaatc
     6001 tatgcagttt taacattatc attgggattt ataagttatg gaccaataca atttagggta
     6061 gcagagatta tgatgctttt ggcattttta gataaaggat atatagtagg tcttacatta
     6121 ggttgttttt tagcaaatgt aatagggcca tatggtgtgc ctgatataat ttttggaaca
     6181 ttagccacat ttataagtgc aagtatgatt tatattacta gaaagttagg taagcaaaat
     6241 aaaagtactt taattatagc ttctttatgg ccgactatca taaattcgat tgtggttggg
     6301 ctaatgttaa atatattctt tggacttccg ttaattttat ctatgataca ggtaggtttt
     6361 ggagagtttg tagtagtaac aattgtagga gtaccatttt tttgtttttt aatgaaaaaa
     6421 tataaaaata ttatcaatat taaattttaa atagtgtata gtaatttaat tgagagtatt
     6481 attggtgaaa agaggtgttt atttgtcaaa aataaaaaga ttgactgatg tttggacata
     6541 tattaatgat aagcaaaagt ttacaataaa agaattatca gaggaattta atctttcagc
     6601 taaaacaata caacgatatt taaaagaatt aaataaaatg ggacttccaa ttcaagcaga
     6661 acaaggtaga aatggtggtt atagggtgct aaataatagc tatatacctc ctgttatatt
     6721 tacagaaaaa gaagttatgg ctatgctatt tgcacttaag tcacttcagg tttataatta
     6781 taagtcaatt gagatagaaa ttaattctat aatgagaaaa gtaagttatg agcgtaagag
     6841 tagtataaaa gtaggtatag aaaatattaa aagatatatt ggatttgtag ctaataattc
     6901 taatgatgga gagtttaaga gtattaatgt aactgagttt tttagagctt cttcagagtc
     6961 tctaatttta aatattaagt ataagtttgg taatcagatt ttggaaaaaa aaatatgtcc
     7021 tataggtata tatttaaata aaggagcatg gttttcacca gcctacgatt gtaaaattga
     7081 taaaataata ttagttcata tagataatgt aattgacata aaaagaacag gagaaagtaa
     7141 aaaaatacat ataaacttgg aagagtggct agatgaagta tataacaatt tatatgaaaa
     7201 taggtcaacc actaaaaaaa ttggtgaaac gatttatcta aatgtattat taactaaaga
     7261 aggtgtttct aaaataaagg acagttctta taatttagat atggaactaa atgaggatgg
     7321 tagtggaaca attagtacat ttataagggt tgaagatgta gattactatg cttctatttt
     7381 atttttaatt ggaaataatg ctaaagttat agaaccaaaa tttctgaata agctacttta
     7441 tgacaaggcc aatgagttaa gatattttta tgaagaaaat atggtataat atttagaaat
     7501 gttgaaatta aataattatt aaggagatat acattataat ggaaaatttt aataagaatg
     7561 ctaaaataat tggtattgga gctgaaggta tcaatataat aaatgaaata gaagaaaaaa
     7621 taaaagccaa tatggatata gaaaaaataa acataaatca agagattgaa aaagaatacg
     7681 ttagaagtct tctagatggt gtagatatat tatttttaat atattcatct gaagataaac
     7741 atattaaaga gattataaaa gctatttcat atatgtcaaa tgagagaaga gttctatcta
     7801 taggaatgga ttgttcagag aaagaaaata aagaagcttt agagcttgga agagaattta
     7861 aaatcaataa cgatagtata tttaaatttg ttaatttaat gaatatcatg gtagagtcta
     7921 tttctgattc ttgcatgata aatatagata ttactgattt aaaagaagct attgtaggag
     7981 ataaaggaat taagtattct tttgaagagt ttgaagatac aaaggaatat agcgagatgg
     8041 ctgacatttt atttgacaga atggaatata ttggtgagga atttataagc aagaaaggaa
     8101 ttgtatttgt tgaaggaagc tcggaatttt ctataatgga acttaatgat ttaataaata
     8161 atattcagag aaagatagaa gaaagttatg aagtaatatt ttctttatat atgaaggaaa
     8221 atctagatgg aaatattaga ataggtttat tgtataatta gtatatttta gatattaagg
     8281 agagtgtata tgtctgaaaa tataagagtt acagagtact ggctaatgga agaatttttt
     8341 tatcaggaaa cagacacacc acagattttt agagaagaaa gtataattgc acttggattt
     8401 gatataaaaa taaatttaaa aaaagtattt ggtatgggaa aaaaatcaga aaagtatatt
     8461 aatgagaatt gtaatgataa agattttata aaaagatttg tgtcaattaa aaaaggagat
     8521 tattttttat ataaggcata tcaaaatata gaagaaaaag ttaaatgtaa tgtgtctatt
     8581 ggcatggttg aagaagattt tgaaaatgga tatgaattaa gtcatatttt agggcataca
     8641 ctaccagtaa aatggatgta taattttgat acagtttcaa attgtaaagt atatacaagt
     8701 gaattttata aattaaataa taatgatatt agctcatatt tagaaattat taattcacaa
     8761 gataagaaat cattgagaaa aagaatagaa gaaagtgaaa ataataaatg gtatttaaaa
     8821 aactattatt ataaaaatgt agataaatgt gtaaatgatg aagaactatt tattcagcct
     8881 actaatctag aacagttaga aaatataaaa aatataaaaa cagatgatta cattttattg
     8941 tataatagcg agaaagtaat ctgtgaaaat gaccatacaa tgaagagtct atttggtgta
     9001 tgtcaagtca tggaaggatt cgataaagag aaagtaagta ctttggagga tggatatttt
     9061 ttccctgtta gatggataga gtatgaaaag gaaaatatag atggaaatgc atgggttata
     9121 tttgaaaata atagcatggt tgaaaagtat gtaaaaagat ttactaataa aataccatta
     9181 aatactatac tttatggacc accaggaact ggtaaaacat acaatacaaa acagatggtt
     9241 tataatctag taaataaaac tagtttaaga aacaggtact atacaggtta cttagattat
     9301 aaaaataatg tagaattttg cacatttcat caatcatatg gatatgagga atttatagag
     9361 ggtcttaagt ctgatggaga aggcaacttt aaaccagaag atggaatatt aaaggatata
     9421 tctatagaag catgttatga tgcattgaaa tttgaaaaaa agatgagttt tgagttagaa
     9481 aaagatagct tgactttaga agctataaaa aagagaaaaa agaagttagt attagaaaat
     9541 atagaatata gtgatagttt taattttgaa gaatcagaaa attatgtttt aataatagat
     9601 gaaattaata gagggaatgt gtctaaaata tttggtgaac taataacatt attggaagat
     9661 gataaaagac ttactaaatc aaatcagact attgtcaaac taccatattc aaaagaaaaa
     9721 ttttttctac ctcccaattt gtatatagtt ggaaccatga acacatcaga taaatcaata
     9781 gcacttttag atatagcttt aagaagaagg tttaattttg aagaaattat gccaaattat
     9841 gatttattgt ctattataga tggcatagat attagaaaaa tgctatatac aatcaataaa
     9901 aggatagagt ttttgtatga tagagaccat gttattggac aagcctatct acttgctgtg
     9961 gaaaatatta atgatatagt agtagtattt agaagtaaaa taataccact tcttcaagag
    10021 tatttttatt atgattcaga aaaagttggt ttgattttag gaggaattgg aagaaatgaa
    10081 aaagataaat acattgttta taaagaagaa cttatagctt ctgagttatt taaaaataat
    10141 aatgcctcaa taatggagag taaagttaac tattgtataa aacaaaacat atctagtgag
    10201 gagttaaaaa atatatatga gtaaaaatat atatgttaag gaaacttatg aatggataag
    10261 agtaggtaat ggtgaaaatg agcttacaga aattgaatat gaaaaattgc ttaaatatct
    10321 agagaacaat aatgatgtat taaaaagtaa tataatagat attaaataca aaaaacttag
    10381 atttataaac tatgtaggta ttatatgttt tgaaaatata atacttgaaa tacttcctaa
    10441 gttgtcatta agtgataatc ttgtaaaaga tagagagata ctattacaga tgctttccat
    10501 atgtaataaa attccaatta taatgaatga aaaaataaga ttaagtttaa aaaattataa
    10561 tctcctaaat ttttttatta tgtatttcat tgagagtatg caaatacaga tgaagaaagg
    10621 tatttatttt gaatatataa ataaaataga gaacttaaat gttataaggg gtaaaatatt
    10681 attaagtaca tatgcaaaag aaaaatgtat ttctccaatg aaaataagat gtgaatatga
    10741 tgaatatagt gaaaataatt tcttgaatca agttcttaaa aaagcatgta tatctatttt
    10801 atgtagaata aatgacaatt caattcaagg aaagattaaa aaaattttaa actattttca
    10861 aaatgtagat ttaatttata tagatagaaa aaaactatta gattataaat tttgtaaaaa
    10921 caatgataga tttagagatt gttatttact agctagactt ctacttttaa atttgtctat
    10981 ggataatagt caagataatg aagaggcatt ttctatatta tttgaaataa atactttgta
    11041 tgaagaatat ataggaattt taatcaaaag tatatgggat aattcattta gagaaacata
    11101 tatacaagat aagagtaaat ttcttttgaa aaatgaacaa acaggtaaga aaaactttaa
    11161 tttaagacca gatatagttt tatatgattt gaaaagtgaa tatgaaatta taattgatac
    11221 taaatggaaa gctattgaag tagattcaaa tgttttttat aggtcaagtg atatatacca
    11281 gatgtatgca tatataacaa catatgaaaa tgcaaaaaga tgtatattat tatatccttg
    11341 tattcaaaaa gataaaaatt atagttcttg gaaattatca gaatcattta agggtaagtt
    11401 tatagaagc
//
LOCUS       DAFNNB010000125        11297 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0126, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000125 DAFNNB010000000
VERSION     DAFNNB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0126"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(81..1367)
                     /locus_tag="L3M60_002751"
     CDS             complement(81..1367)
                     /locus_tag="L3M60_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6227394.1"
                     /translation="MIVGIIGPSDSGFKIKDDLKQIDNDLKTKIYVREKVVDTIEVIS
                     KCEDECDAIIFTGCAVYEFIKSKYEISKPHSFVPRSGTSIMKAFWAIKSANIKLDKFS
                     IDVVDRYVVEDALKEFDIKATSVFCNPFSLEVGESELVEWHIKLFEENKTDIMLTGFG
                     AVYNELKERGYPVFRLQATIQLIKESYDKVKSEYALSKARFSQIAVEILSLIDYKEKI
                     DNYYSDMIKKSDMDKLVVNYVRSIQGSLFLLGRNDYVVFVHKGVADNEYNYDKLFKLK
                     REIKDMGFSLSIGIGTGVTAYQAENNAHKALRYSIDSKEIGIYLVDEDENIRGPLASE
                     NELNYSLMLSDEKVIEISKKTGMSCESVAKIIAISENRKSKIYDSKELAEYLNVSERS
                     ARRILNKIVNAGLGRICAKETSIGGGRPKNITEILF"
     gene            complement(1490..4588)
                     /locus_tag="L3M60_002752"
     CDS             complement(1490..4588)
                     /locus_tag="L3M60_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBN6227395.1"
                     /translation="MDVINLIDELDEIVYVSSLDTYELLYINEIGKKALRINDISNMK
                     CYEAIQGRDSPCEFCTNSILKEDGYYVWENTNTLINRHFILKDKLIKWEGKLARIEIA
                     LDITEKENISKSISEKLEIKELLVGCIKHLITESSFSDAVDLVLSSIGKFHKADRAYV
                     FEVSEDRKKVINTYEWCNEGVDEQKQLLTNLDIKELKNWFLLFKQKGFIIIDDVEDIK
                     EEHPMEYEILKPQKIKSLMASSLKEDDKIIGFIGVDNPRKAVGDTSLLTSLSYFLLTE
                     MKKRKVENKLNYMSYYDFLTSAYNRNRYIKYIDTFQKDKVKSIGVVFIDINGLKDIND
                     NFGHASGDAIIMSVSVMIQKYFHSYYVYRIGGDEFVVLCEDISKQLFEELVSWLTEEL
                     KDNMECSAAVGSIWSEDDIDIHKLIKVADERMYQDKKRYYNKIELRNNEMKVVSDLSD
                     STNNISLKCRDLDNEVYFDDLMIKGYSKAYEKLAYNTYDIILSICMDTNLITPIYQSE
                     SNILNFPIKENIEKIFSYIKKHLIHPDDIILFDDNLLLKSIDSPNNSKQGDLELNFEY
                     RHLGIDEKYHWALLYILKISSKNNLGEKQTHILVAIKNIDYLMQIHKNQKDTLTGLYN
                     YESFCEQTSLMLKRYPGKRYAIIMMDIDKFKVINDIYGIRGGDKALIYVAELIQKNIG
                     SNGICCRMYADVFCIFYESYSDREIHKVIYNIKNNLNRKKFEYMLVPCFGVYKILDNH
                     IPITSACEMAGYAHKKVKKQSINHYIFYDDTIRNDALEEKHMEREMEYALENGQFHVY
                     LQPKYNLNTNKIVGAEALVRWVHPEKGIIPPIKFISLFEKNGFIIKLDMYVWEQVCSL
                     IRKWIDLGEEPVPISVNVSRLHLHNPHFKEIVLSLINKYGIPKSFLELELTESLFIKN
                     IKLFTKVIADLRMDGIVLNMDDFGSAYSSLNMLKNINIDTLKIDCGFFNGEGITDREK
                     IVLKHTIAMAKDLDMDVVAEGVETKEQAEFLVSLDCVVIQGYYYCKPIPSDDFEKLLY
                     QK"
     gene            5293..5451
                     /locus_tag="L3M60_002753"
     CDS             5293..5451
                     /locus_tag="L3M60_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227396.1"
                     /translation="MKIFSFMLYVVGICFCIAGFIGHYPAFAGATLSFFATTMIAVNQ
                     GKTKKKMK"
     gene            complement(5740..6444)
                     /locus_tag="L3M60_002754"
     CDS             complement(5740..6444)
                     /locus_tag="L3M60_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227397.1"
                     /translation="MEIKPLVLIVEDDKPICKFIKVSLETQNYRCVETDNGGTAISLI
                     HSLDPDLIILDLGLPDIDGIEVIGRVRACAKTNKIIVVSAREHERDKVEALDGGADDY
                     LTKPFSVTELLARVRVALRNKAQQGNINNDAPKSFEVKNLKIDYENHTVLINDEEIHL
                     TPIEYKIIELMSKYSGRVLTHKFIIDKVWGNYYESENQSLRVFMASIRRKIEKNPAQP
                     EYILTEVGVGYRMADE"
     gene            complement(6435..8042)
                     /locus_tag="L3M60_002755"
     CDS             complement(6435..8042)
                     /locus_tag="L3M60_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HBN6227398.1"
                     /translation="MKKERLQHEDLKEYDYDEDVYLSKKIIIKDFIKTILVMTIATGI
                     SVVFEKVGFNESNIILVYILGVLFATSITYGYFFGILSSVIGVLSFNFFFTHPLYTFL
                     AYRKDYPMTFCIMLMAAIITSTLTSRIKREVRLSHKREKMIRLLYENNKTLLKAKNKN
                     QIIEFCGSNLVNILNRTVIVTIADENNNLGKPSIYICNNDGSENIFHSVLEKEAIKEA
                     FRTGNPVGIGTKYCKNSSAYYFPIKGQESNVLGVIGIACFKIDIITENEKILLEAVST
                     QVALAIDRENLFEKNKKANLEAERERLRGNLLRSISHDLRTPLTSILGSSSTILENDD
                     VIDKETRVELLKNIYEDTSWLTHSVENILSMTRIDEGKLDIEKRPEVVDEIIAESILR
                     VKKFANSRDIKTNIPDEIIIVHVDVLLIEQVLVNLIDNAIRHTPKNSKIELTVKKESS
                     QVLFEVADNGKGIPNEDIGNIFNRFYTKNKSRNLERRGIGLGLEICKSIVEAHGGEIV
                     AFNNPSGGATFRFSIPVYDEEGKDKWK"
     gene            complement(8298..9476)
                     /locus_tag="L3M60_002756"
     CDS             complement(8298..9476)
                     /locus_tag="L3M60_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227399.1"
                     /translation="MEKLSDKRRVKAVSMETFVFIVLLAVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLDTVFLIMAMAVLAGALSALLSEFGVISLVNKIFKGLMRPIWGLPGASIA
                     GVVATYLSDNPAIIPFAKDKTFTQYFKKYQVPALCNLGTAFGMGLIVTTFMIAQGKEY
                     VLPAIIGNVGAIIGSIISVRIMLTFTKKYYNYDPKNDTEKQINDKGAKLEEFREIRDG
                     NVFQRTLDAILEGGKLGVEMGMAIIPGVLVVCTLVMLLTFGPSTDPATGQAVYTGAAY
                     EGIKLLPAIGDKISFIIEPLFGFTSPEAIAFPVTALGAVGAAISLVPEFIKSGAITPN
                     DIAVFTAMGMCWSGYLSTHIGMMDALDARPLAGKAILSHTIGGLCAGICAHFIFMLVG
                     "
     gene            complement(9700..10194)
                     /locus_tag="L3M60_002757"
     CDS             complement(9700..10194)
                     /locus_tag="L3M60_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBN6227400.1"
                     /translation="MHLFIEVIAAFFTALSFGVLFNMKGKNLILAGIGGSIAWFSYKF
                     LLNMGVTENLCFFIATVCFAIYCELCARIYMTPATTLSVCCLIILVPGYGIYNTMYSV
                     LTNNYIKAVEYGVSTLSCASSIALGLVFITTVFRKVNLYGVLTRIKENEKYKNSINKI
                     KQQK"
     gene            complement(10195..10956)
                     /locus_tag="L3M60_002758"
     CDS             complement(10195..10956)
                     /locus_tag="L3M60_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HBN6227401.1"
                     /translation="MDIDRILNFSSNAGKAMLQSGGETYRVEETIARICQSFGIDHVD
                     VFATPTAVMASVFVDGKLHSAIKRISSRRVDLNLVHEVNSLSRAISINNLDIELCEKI
                     LDEISEDNYYSDLITIFFAGIAAATFSILFGGNVEEFIAAFTVGILTKFVIMYLSKSS
                     LNDFFVNAIGAVIIASCSILILKLGFIKTLDHLIAGAIMLLVPGLALTNALRDLLDGQ
                     LISGLAKLAEVFFIGVSIAVGMFLVLGLYFKLGGI"
BASE COUNT         3762 a         2046 c         1139 g         4350 t
ORIGIN      
        1 aataagcttt aatatattat atagtaattt gttgtagaaa tttatatgat ataatgattt
       61 atacagcttt ataaagtatc ttaaaacagt atttcagtaa tattttttgg tcttcctcca
      121 ccaatactag tctcttttgc acatattcga cctaaacctg catttactat tttatttaat
      181 atacgtcttg cacttctttc acttacattt agatactctg ctaactcttt tgaatcataa
      241 atcttacttt ttctattctc acttatagca ataatttttg caaccgactc acaactcatt
      301 ccagtctttt tagatatttc tattaccttt tcatcagata gcatcaaaga ataatttaat
      361 tcattttctg aagctaaagg tcctcttatg ttttcatctt catctactaa atatatacca
      421 atttcttttg aatctatgga gtatcttaat gctttatgtg cattattttc agcctgatac
      481 gcagttactc cagtaccaat tcctatacta agagaaaagc ccatgtcttt tatctctctc
      541 tttagcttga atagcttatc ataattatac tcattatcag ctaccccttt atgaacaaat
      601 acaacataat catttcttcc aagtaaaaat aaggaacctt gaatacttct tacatagttt
      661 actacaagct tatccatatc agattttttt atcatgtcag aataatagtt atctatcttc
      721 tctttataat ctattagact caatatttcg acagcaattt gagaaaatct tgccttgctt
      781 agagcatatt cactctttac cttatcataa ctttctttga ttaattgtat agtagcttgt
      841 aaacgaaata caggatagcc tctttctttt aattcattat atactgcacc aaaaccagtt
      901 aacattatat ctgtcttatt ttcttcaaat aattttatat gccattctac aagttcagac
      961 tctcctactt ccaaagagaa tggattacaa aatacactag ttgctttaat atcaaattct
     1021 ttaagcgcat cttccacaac atatctatca actacatcta tactgaattt atctaatttt
     1081 atatttgcac tttttattgc ccaaaatgcc ttcattatac tagtcccact tctcggtaca
     1141 aatgaatgtg gcttacttat ctcatactta gattttataa attcataaac tgcacatccg
     1201 gtaaatataa tagcatcaca ttcatcttca catttagaaa taacttctat tgtatctacc
     1261 actttttctc ttacatatat tttagtcttt aaatcattgt ctatctgttt taaatcatcc
     1321 tttatcttaa acccagagtc tgaaggacct ataataccca ctatcataaa cttcgctcct
     1381 aatccataaa tattatactt tttaaaaatt atatcctcac tattttttgc ttctatttaa
     1441 ttattttgaa ctactccatc ctacctacgt ttagaatgga ataattcgct tatttttgat
     1501 aaagtaactt ctcaaaatca tcacttggaa taggcttaca ataataataa ccttgaatta
     1561 ctacacaatc caaacttact aaaaattcag cttgttcttt tgtctctaca ccttctgcca
     1621 ctacatccat atctaaatct tttgccatgg ctatagtgtg ctttaaaact attttttctc
     1681 tatctgtgat tccttcacca ttaaagaaac cacaatctat ctttaatgta tcaatattaa
     1741 tattttttaa catatttaat gaagaatatg ctgaaccaaa gtcatccata tttaaaacaa
     1801 taccatccat tctaaggtca gctattacct tagtgaataa ttttatattt ttaataaata
     1861 gactttctgt aagctctaat tctaaaaaag attttggtat accatattta tttattaagc
     1921 ttaaaacaat ttctttaaaa tgtggattat gcaaatgtaa tcttgataca tttacagaga
     1981 taggaactgg ctcttcacct aaatctatcc attttctaat caaagaacat acttgttccc
     2041 atacatacat gtctaatttg ataataaacc cattcttttc aaatagtgaa ataaatttaa
     2101 ttggaggtat tatacctttt tctggatgaa cccatctaac caatgcctct gccccaacaa
     2161 ttttatttgt attaagatta tacttaggtt gtaaatatac atgaaattgt ccattttcaa
     2221 gtgcatattc catttctctt tccatatgtt tttcttcaag tgcatcattt cttatagtat
     2281 catcataaaa tatataatga ttgatacttt gtttctttac ttttttgtga gcatatcctg
     2341 ccatttcaca tgcacttgta attgggatat ggttatccaa aattttataa accccaaaac
     2401 atggtactaa catatattca aattttttcc tattcaagtt atttttaata ttataaatta
     2461 ctttatgaat ctctctatca gagtaactct cataaaaaat gcaaaataca tctgcataca
     2521 tacgacaaca tataccatta gaaccaatgt ttttttgaat caattctgca acatatatta
     2581 aggctttatc tcctcctcta attccataaa tatcattaat tactttgaac ttatctatat
     2641 ccatcattat aattgcatag cgcttgccag gatatctttt cagcattaag cttgtttgtt
     2701 cacaaaaact ttcataattg tataatcctg ttaatgtatc cttttggttt ttatgtattt
     2761 gcataagata atctatattt ttaatagcaa ctaaaatatg tgtttgtttc tcacctaaat
     2821 tatttttact gctaatcttc agaatataaa gtaatgccca atggtacttt tcatctattc
     2881 ctaagtgtct atactcaaaa tttagttcta aatcaccttg tttgctatta ttaggactat
     2941 caatagattt taaaaggagg ttatcatcaa ataatattat atcatcaggg tgaatcaaat
     3001 gctttttaat gtatgagaaa attttctcaa tattctcttt tataggaaaa tttaaaatat
     3061 tgctctctga ttgataaatt ggagtaatta aatttgtatc catgcaaata gataaaatta
     3121 tatcatatgt attataagct aatttttcat aagccttaga ataccctttt atcattaaat
     3181 catcaaaata cacttcatta tctaaatctc tacatttaag tgaaatatta tttgttgaat
     3241 cgcttaaatc actaactacc ttcatttcat tatttctaag ttcgatttta ttatagtatc
     3301 gtttcttatc ttggtacatc ctctcatcag caactttaat taacttatgt atatcaatat
     3361 catcttcact ccaaatactt cctactgctg cactacattc catgttatct ttaagttctt
     3421 cagttagcca acttactaat tcttcaaata attgttttga gatatcttca cataatacaa
     3481 caaactcatc tcctccaata cggtaaacat aataagaatg aaagtatttt tgtatcatta
     3541 cactgactga cataatgatg gcatctccac tagcatgtcc aaagttatca ttaatatctt
     3601 ttaatccatt tatatctatg aatacaactc ctatagattt aactttgtct ttttgaaaag
     3661 tatctatata cttaatatat ctatttctat tatatgcact agttaagaaa tcatagtagc
     3721 tcatataatt taatttattt tctacttttc tttttttcat ttcagttaat aaaaaataag
     3781 atagggatgt caaaagagag gtgtctccaa cagccttacg tggattatcg actccaataa
     3841 atcctattat tttatcatct tcttttaatg aagacgccat taaacttttg attttttgag
     3901 gttttaaaat ttcatattcc attggatgct cttcttttat atcttctaca tcgtcaatta
     3961 ttataaatcc tttttgctta aataataaaa accaattttt taattcctta atatcaagat
     4021 ttgttagtaa ctgtttttgt tcatcaacac cttcattaca ccattcatat gtattaatta
     4081 ctttttttct atcttctgat acttcaaaaa catatgctct atcagcttta tgaaatttac
     4141 caatactgct taatacaagg tctacagcat cagaaaaact agattctgtt ataagatgtt
     4201 taatacatcc aactaatagc tcctttatct ctaacttttc tgatattgat ttacttatat
     4261 tttccttttc tgtaatatca agagctattt ctatccttgc aagtttacct tcccatttaa
     4321 ttaacttgtc ctttaatata aagtgtctat ttattagagt atttgtattt tcccatacgt
     4381 aatatccatc ttcttttaaa atagaatttg tacaaaattc gcaaggagag tctctacctt
     4441 gtattgcttc ataacatttc atattagata tatcattaat cctcagtgcc tttttcccaa
     4501 tttcattaat atataaaagt tcatatgtat ctaaagaaga aacatataca atttcatcca
     4561 attcatctat taaatttatt acatccattt atatatatct ccttttattc attttcccag
     4621 attgaatttt aaatctttta aaattttgct atgtatttag caatgatgtt atgcttcttt
     4681 atattttaaa atagcacctt aataaagttc tattttttag tcaatagagt tattatcgtg
     4741 tcaattgtac agtagagctt tgtaatactt aatttaatat atctataatt aattatcgta
     4801 ttgtgtattt acaattaatc atcatataaa taataaatct ccacttcaat taagtattcc
     4861 aatcatatag aaagataata cttcattcat ttaaaaatat aaaactatta tatattaaga
     4921 tttctcaata ttaataagtt atttagtttg ttacttttat atattatata atatcacaaa
     4981 ggctgacata aaaagataaa aagggcaatt tttttatatc aaaaaagagt taaattgatt
     5041 tatccatata aatattatat ctaacaattc tacttacttt tcttacactt attagcatat
     5101 agttttcgag agcttattac aaactaacac ttagcacacc ttaaaaaata tggtctgata
     5161 agtaatcata caaagaatac acgataaacc ataaaatatg acatactttt tcacgtcatc
     5221 aaaactgatg atttcactat taatatatga taaaatatgc ttaataaatt aaattttaaa
     5281 gggagaatag ttatgaaaat atttagtttc atgttgtatg tggtgggaat atgtttttgt
     5341 attgctggat ttataggaca ttacccagca tttgctggtg caacattgag tttttttgca
     5401 actacaatga tagccgtaaa ccaaggcaaa actaagaaaa aaatgaaata agtaaatgcc
     5461 aactttacaa gctagtatta cacaagtagt tagtctaaaa ttggcttact ttttctatta
     5521 tagaaataca tcatcaactt tacattctct tggaaaaata aaaatagcta tgtaaattca
     5581 atattagata gactaactct ctatccattt cattataaaa tatacatagc tatttgtatt
     5641 cgatttaaaa tacttatatt ttcttatata acaaataatt tctatctgat tactactcat
     5701 attcatttgg tgaccactca tcaactaccc atttagtatc tattcatctg ccatacggta
     5761 acctacccca acctcagtca aaatatattc tggctgagca ggattctttt caatttttct
     5821 tcttatactt gccataaaaa ctctaagtga ctgattttca ctttcatagt aatttcccca
     5881 aactttatct attatgaact tatgagttaa aactcttcct gaatacttag acatcaactc
     5941 aataatctta tattcaattg gagttaaatg tatttcttca tcattaatta acacagtatg
     6001 attttcataa tctatcttta ggtttttgac ttcaaaagat tttggagcat cattatttat
     6061 attgccttgt tgagctttat ttctaagagc tacacgtact ctagccaaaa gctctgttac
     6121 actaaaaggt ttagttaaat aatcatctgc ccctccatct aaagcctcca ccttatccct
     6181 ctcatgctcc cttgctgaaa caacaattat tttattagtc ttggcacatg ccctaactcg
     6241 tcctattacc tcaattccat caatatctgg tagtcctaaa tccaaaatta tcaaatctgg
     6301 gtcaagagaa tgtataagtg atatagctgt tcctccatta tctgtctcta cacacctata
     6361 attttgagtt tcaagagata cttttataaa tttacaaatt ggcttatcat cttctacaat
     6421 taaaactaat ggctttattt ccacttgtct tttccctcct catcatacac aggtatgcta
     6481 aatctaaatg tagctcctcc agaaggatta ttaaaggcga caatttctcc accatgagct
     6541 tctactatag atttacatat ctccaatcca agaccaatac ctcttctttc aagatttcta
     6601 gacttattct tagtataaaa cctattaaaa atattaccaa tatcttcatt tggtattcct
     6661 tttccattat ctgcaacttc aaatagcact tgacttgact ctttttttac agttagttct
     6721 atcttagaat ttttaggtgt atgccttata gcattatcaa ttaggtttac taaaacttgt
     6781 tctatcaata atacatccac atgcacgatt attatctcat ctggaatatt ggtttttata
     6841 tctctactat tggcaaactt tttgactctt aatatagact ctgctattat ttcatcaaca
     6901 acctctggtc gtttttcaat atccaatttt ccttcatcta ttctggtcat actaagtata
     6961 ttctcaacag aatgagttag ccaactcgta tcttcatata tattttttaa taattccact
     7021 ctagtttctt tatctatcac atcatcattt tcaagtatag ttgaagatga acccaatata
     7081 cttgtaagtg gagttcttaa atcatgagaa attgacctaa gcaagtttcc tcttaatctc
     7141 tctctctctg cctcaagatt tgctttttta tttttttcaa ataaattttc tctatcaatt
     7201 gccaacgcaa cctgtgttga aactgcttct aatagtatct tttcattttc tgtaataata
     7261 tctatcttaa aacacgcaat acctataaca ccaagaacat ttgattcttg accttttata
     7321 ggaaaataat atgcactgct gtttttacaa tattttgtcc caattccaac tgggttaccc
     7381 gtcctaaaag cttcttttat agcttccttt tctaatactg aatggaatat attctcactt
     7441 ccatcattat tacaaatata aatacttggc ttacctagat tattattttc atctgcaata
     7501 gtcactatta cagttctatt taatatattt actaaattag acccacaaaa ttcaataatt
     7561 tgatttttat tttttgcttt aagcaacgtt ttgttattct cataaagtaa tcttatcatt
     7621 ttttctctct tatgagaaag tctcacttct ctttttatcc tagaagttaa agtacttgtt
     7681 ataattgctg ccatcagcat aatacaaaag gtcatagggt aatcttttct ataagctaaa
     7741 aaagtatata atggatgtgt aaagaaaaaa ttaaatgata gcactcctat tactgaagaa
     7801 agtattccaa aaaaatatcc ataggttata cttgtagcaa atagtacacc taatatatat
     7861 actagtataa tattagattc attaaaacct actttttcaa aaactactga tattccagta
     7921 gcaattgtca taactaatat agtttttatg aagtccttaa taattatctt tttactgaga
     7981 taaacatctt catcataatc atactctttt aagtcttcat gttgtagtcg ttcctttttc
     8041 atttactaca attcctcctt attgttactg tttaaattgg ctctatttta tataatacaa
     8101 ttttatcata ataaattcaa tcaatacatt taattaatgt atttaaatta ctaaaaggtg
     8161 ctgtcttaaa atgaaaataa atctctattt caaaacagca cctctatata tttaaattga
     8221 taatttaatt tttatatact taaattatta gttttattta atttcataaa ttataatact
     8281 tgatttatcg aatattctta tccaacgagc ataaatataa agtgggcaca tatacctgca
     8341 cataaaccac ctatagtatg cgaaagtatc gctttaccag ctaatggtct tgcatctaga
     8401 gcatccatca tacctatgtg tgtacttaaa tatccactcc aacacatacc cattgctgtg
     8461 aatactgcta tatcattagg agttatagcg cctgatttta taaactctgg tactaaagat
     8521 attgctgctc caacagctcc taatgcagtt acagggaatg ctattgcttc tggtgatgta
     8581 aatccaaata atggctctat tataaagcta atcttatcac caatagcagg taataatttt
     8641 ataccttcat atgcagctcc tgtgtaaaca gcctgacctg tagcagggtc tgtagatggt
     8701 ccaaaagtta ataacataac taatgtacaa actactaaaa cgcctggtat tatagccata
     8761 cccatttcaa cacctaactt accaccttca agtatagcat ccaatgttct ttggaataca
     8821 ttaccatctc ttatttctct aaactcttca agttttgcac ctttatcatt tatttgcttt
     8881 tcagtatcat ttttagggtc ataattataa tactttttag taaaagtcaa cataattcta
     8941 acactaataa tactacctat tattgcgcca acattaccta ttatagctgg tagtacatat
     9001 tctttacctt gagctatcat aaaagttgtt acaataagtc ccataccaaa ggctgttcct
     9061 aaattacata aagcaggtac ttgatacttc ttaaaatatt gtgtaaatgt tttgtcttta
     9121 gcaaatggta taatagctgg attatcagat aaatatgttg caactactcc tgctatacta
     9181 gcaccaggta agccccatat aggtctcata agccccttaa atatcttatt aactaaagat
     9241 attactccaa actctgaaag caacgcacta agtgcacctg caagaactgc catagccatt
     9301 attaaaaata ctgtgtctaa taaaagcgca tgtgctgtac tcattattac tttaaacatc
     9361 attcctgcac ccatgatgct tcctacatat ccaaatccta ccgccaatag tacaataaat
     9421 acgaaggttt ccatacttac tgccttgact cttctcttat ccgacagctt ttccattttg
     9481 aaatcttccc ccttttaatt ttaaattaag gttcctaaca ttggtacaaa ctttttggta
     9541 caaactgagt tctaaaactt acactcgtaa tgcaagttgt cagttttttg aaataaaaaa
     9601 attacgttca tagaataaga atatcatctt tattttggat actcttattc tattttatgt
     9661 tattatatta tcatcctttt gtttaaaagt caattttttc tatttttgtt gtttaatttt
     9721 atttatacta tttttatatt tttcattttc ttttatcctt gtaagcactc catataagtt
     9781 gacttttcta aatacagtag ttataaatac caaacctaat gcaattgagc ttgcacatga
     9841 taatgtactc acaccatatt ctacagcttt tatataatta ttggttaaaa cagaatacat
     9901 agtattatat attccataac caggtactaa tattattaaa caacaaacac ttaatgtagt
     9961 tgctggagtc atataaattc ttgcacataa ctcacaatat atcgcaaaac aaactgtagc
    10021 tataaaaaaa cataagtttt ctgttactcc catatttaaa agaaatttat atgaaaacca
    10081 agctatactt ccacctattc ctgctaatat taagtttttt cctttcatat taaatagcac
    10141 tccaaagctt aaggcagtaa aaaaagctgc tattacttct ataaacaaat gcacttatat
    10201 cccccctaac ttgaagtaca atccaagtac caagaacatt cctactgcta tagatacacc
    10261 tataaaaaat acttctgcta attttgcaag accagaaatc aactgtccat ctaacaaatc
    10321 tcttagtgca ttagtcaaag caagcccagg aactaaaagc attattgctc ctgctattag
    10381 atggtctagc gtctttataa atccaagctt taaaatcaaa attgaacaag aagctataat
    10441 aactgctcca attgcattta caaaaaagtc attaagggaa gatttactta agtacataat
    10501 aacaaactta gtcaaaatcc caacagtaaa tgcagctata aattcttcca catttcctcc
    10561 aaataatatc gaaaaagttg ctgctgcaat acctgcaaaa aatattgtta ttaaatctga
    10621 gtaatagtta tcttcactta tttcatccaa aattttctca cataattcaa tatctaaatt
    10681 atttatactt attgctcttg ataatgagtt aacttcatga accaagttta ggtctactct
    10741 tcttgagctt attcttttaa ttgctgaatg taattttcca tctacaaata cagatgccat
    10801 cacagctgtt ggagtagcaa atacgtccac atgatctatt ccaaaagatt gacatattct
    10861 agctatcgtt tcttcaactc tataggtttc tcctccgctt tgcaacatag ctttacctgc
    10921 atttgaggaa aagtttaaaa ttctatctat atccacaaaa catatctccc attcttttat
    10981 agtattattc atattttaat acaagttatt atttatttaa actatttcaa ataaataata
    11041 tccaatattt acattttatg aaaaaacttg ttttttatgc ttgtcaaaac caaattttaa
    11101 ggttgtttac accataaaat ggctgaatta atatttgaca aacactttca accatttata
    11161 taaccttcta aattgtataa ctttttacta caaataaaat tatatctctt tttattcttt
    11221 ctcttggctc tttaagttta cagcaacttc atatttatat atgtacaata taaaatatta
    11281 tttttatgat acaaaat
//
LOCUS       DAFNNB010000126        11096 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0127, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000126 DAFNNB010000000
VERSION     DAFNNB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11096
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0127"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            150..1301
                     /locus_tag="L3M60_002759"
     CDS             150..1301
                     /locus_tag="L3M60_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBN6227402.1"
                     /translation="MEIYLDNSATTKPYQEVIDKMVYALNTEYGNPSSVHRKGVEVEK
                     GIKEVRQDVAKSLGAKEKEIYFTSGGTECNNTIIRGITSLNKKRKNHIISTNIEHPSV
                     LNTLKDLEEDGFEVTYLEVGKDGKINIEDLKSAIKSTTCLVSMMHVNNEIGTIQPIGE
                     VGKYLKGLKEKIYFHVDAIQSYGKINFRPSKYNIDFMSVSAHKFHGPKGIGFMYIKEN
                     NRLKPMLTGGGQEIGIRSGTENVPGIYGLGEAVRILNKDLDAVISKVNNLKNTLKNEI
                     IDNIEDIKINSPEDGVCHILNVSFRGTKGEVLLHYLEQKGIYVSTGSACSSKKKGSHV
                     LNAIGLTNEEINGTIRFSLSDMNTEEEMLEAVKVLKESICDLRSIMKRK"
     gene            1347..2522
                     /gene="thiI"
                     /locus_tag="L3M60_002760"
     CDS             1347..2522
                     /gene="thiI"
                     /locus_tag="L3M60_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBN6227403.1"
                     /translation="MVYNILIVKYGEIGVKGKNRYIFENRLIRNIRNMLKPIGKFNVY
                     KEYGRIYVDLEDYDYEEVIEEVRKVFGIVGVCPGVRAKKDYDTLKEIALKMLEEKIDA
                     GYKTFKVESRRGDKSFGLTSQEMSMDIGGYLLSKVGDKINVDVRNPEVKIKCEYREFH
                     TMVYSDTIPGYGGLPLGTNGRAMSLLSGGIDSPVATWMVAKRGMEVEAVHFHSYPFTS
                     ERSQEKVKDLAKILAKYCGRVRLHKVNILEIQKAIGENCNEEEATILSRRFMMRIAQR
                     LSEKRHCDALITGESIGQVASQTIQGLTCTNAVVDLPVFRPLIAMDKSDIVDIAKKIG
                     TFETSIVPEEDCCSVFSPRKPVTKPRLEKIEKSETALDIEKLVQDAIDKIEVEDIEF"
     gene            2658..3461
                     /locus_tag="L3M60_002761"
     CDS             2658..3461
                     /locus_tag="L3M60_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBN6227404.1"
                     /translation="MNKKGFTLIELLVVISIIGILVIVAVPALFRNIEKSKAVTCLSN
                     RENIKTQIVIAMAEEPSKDKNKVIKDVLENKDGKYFETEPKCKSGGIYSATFDDGYDG
                     ITGGESIAKVYVTCTEHPDGVEMARDVHQSMKDLIASFAQDPSIIPGASKSNDDFRKY
                     LLDNKYKKGWPTIPDEFKAKYGLSKDTLYIQPYAYNPTKPDATVVVFANNKTGGNWYT
                     SLVYDYDEGRWYKGKNGISVAGRSWDVDTDSVKSVKTEIHSKEGWGPLN"
     gene            3704..4345
                     /locus_tag="L3M60_002762"
     CDS             3704..4345
                     /locus_tag="L3M60_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBN6227405.1"
                     /translation="MINNFQDNQTINLIQSRRSIRKFTTEQISDEQVNTLLHCAFAAP
                     SGCNKQPWHITVVQDQKLLKAISDDTLSRIHEVSNVEINKNFKLFYGAPTVLFISYDE
                     SSSWAPYDVGILTGNITTAAQALGLGSCIIGMIRGLFTPVEQGDIEGLVSVLDKEDVK
                     ESESIKMKFDTNKKYREILDIPEGYSVPFGIAIGIPDGNLPNAREVVYKVSRV"
     gene            complement(4744..5478)
                     /gene="pflA"
                     /locus_tag="L3M60_002763"
     CDS             complement(4744..5478)
                     /gene="pflA"
                     /locus_tag="L3M60_002763"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HBN6227406.1"
                     /translation="MIKGKIHSIETFGTVDGPGIRYILFFQGCPLRCKYCHNRDTWDI
                     KSGKEYTVDEIITDALKYTSFMKFSDGGITASGGESTLQPEFLSELFKKAKENDIHTC
                     LDTSGFVDIETIDPVLDNTDLVLLDLKHMVEEKSIDLTGVGMDKALKLARHLESRNIP
                     VWIRHVLVPGITDDVDNLEKLGKFVATLKNVERFELLPYHSMGIHKWENLGIDYELKD
                     VPDATKEDVQKASEIISKFGVKVYNS"
     gene            complement(5638..7869)
                     /gene="pflB"
                     /locus_tag="L3M60_002764"
     CDS             complement(5638..7869)
                     /gene="pflB"
                     /locus_tag="L3M60_002764"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBN6227407.1"
                     /translation="MNAWQGFKTGRWTKEINVREFIQLNYSPYEGNDSFLAGATENTK
                     KLWDKAMVLFKKERENGGTLDVDTKTVSGIAAYAPGYLDKELETIVGLQTDAPLKRAV
                     MPYGGIKMVENSCEAFGYELDPEIKDIFTKYRKTHNQGVFDVYTPEMRAARKSGIITG
                     LPDAYGRGRIIGDYRRVALYGVDALIEDKNEQKKSLEVSCMDEEVIRLREEITEQISA
                     LNELKKMAESYGFDISKPATNSKEAVQWLYFGYLGAVKDQNGAAMSLGRTSTFLDIYF
                     ERDLKAGIVTEEELQEYMDHFVMKLRMVKFLRTPDYNNLFSGDPTWVTECIGGMGIDG
                     RTLVTKNSFRMLNTLYTLGPSPEPNLTVLWSTKLPQGFKDFCSKVSIDTSSVQYENDD
                     LMRAYWGDDYGIACCVSAMRIGKQMQFFGARVNLAKTLLYAINGGVDEKSGVQVGPRF
                     EPITSEYLDYDEVMSKFEPFTDWLATLYVNTLNVIHYMHDKYSYEALEMALHDRDIFR
                     TMACGMAGLSVCADSLSAIKHAKVKTIRNEQGIAVDFEIEGDYPKYGNNDDRVDSIAV
                     ELVESFMNKIRKNKTYRNSYPTQSILTITSNVVYGKKTGNTPDGRRAGAPFAPGANPM
                     HGRDTNGALASLSSVAKLPYEHAQDGISNTFSIVPAALGKDMTERINNLSAMMDGYFA
                     QNAHHLNVNVFDRATLEDAMEHPEEYPQLTIRVSGYAVNFIKLTKEQQLDVINRTFHG
                     KMA"
     gene            8162..9223
                     /locus_tag="L3M60_002765"
     CDS             8162..9223
                     /locus_tag="L3M60_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBN6227408.1"
                     /translation="MEKRKSIHLHKLNIYLIGGGDILFITVVLFLVGFLLITKGADIF
                     INCTVEIGRKTNISEIILGATIVSFATTLPEFTVSLLASIDGHTTMSLGNAVGSIICN
                     TGLALGLVVFLSPFNVDKKMFFSKSLLLIVSVIVLILLSLDGVITRGDSLLLIIILIF
                     YMVNNYRSVVGKSDTKSRNIKNNTIKDISTKKGKNYRGFSVLEIVKILLLFATGLIMM
                     IIGSQILIESGVRIASFLNIPQGIVSLTIIALGTSLPEIVSSITAIRKNHHEISVGNI
                     LGANILNIVSVIAISAIPNNIPILSQNRQLDIPFMILLILIVIIPTLKSNRLSRIQGI
                     LMLFTYFLYISILYFMYII"
     gene            9314..10927
                     /locus_tag="L3M60_002766"
     CDS             9314..10927
                     /locus_tag="L3M60_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBN6227409.1"
                     /translation="MNITKFEDLPISEGIKKAIAEMGFEEPSPIQAQSIPAILSGKDV
                     IGQAQTGTGKTAAFSIPILETIDPNNRSLQAVVLCPTRELAIQVSTEIRKLAKYSHGI
                     KTLPIYGGQPIDRQIKSLKSGVQVVIGTPGRTIDHINRKTLKMDNVKMIILDEADEML
                     DMGFREDIEMILSKIPEERQTTFFSATMPRGILELTKRYQKDPEHIKVVRKELTVSNT
                     KQYYIETRSSNKLEVLCRLVDVYDPKLSVVFCNTKRKADELVGDLQARGYFADALHGD
                     LKQTQRDIVMDKFRNGTIDILVATDVAARGIDVDDVECVFNYDLPQDEEYYVHRIGRT
                     GRAGREGMSFTFVFGKEMRKMKDIERYTKSKLIKHNIPTITDVEEKKVGTFFAQVKQT
                     IEEGHLTKQLQWLENFCNDEDYAMVDIAAALVKLSLGEEMKEEIIEEKPRRERGDRKG
                     GTGAKDGMIRLFINIGRNQRVQAKDIVGAIAGEVGIPGKVVGTIDIYDKYTFVEIPKK
                     DAKTVIEKMKDIKIKGNKINIEKANKKKK"
BASE COUNT         4178 a         1401 c         1869 g         3648 t
ORIGIN      
        1 ttgtaataaa gtgaagaaac tagcttttca tgaaataata ttttataatt taagattgtc
       61 tttttaataa agattaacaa aaaaaatact ataattaatg gtataatagt tagagttgaa
      121 gattttaaat aaaggtaggt gtaattaagt tggaaatata cttagacaat agcgcaacta
      181 caaaacctta tcaagaagtt atagataaaa tggtttatgc acttaatacg gaatatggaa
      241 atccttcttc tgtccataga aaaggtgtag aagttgaaaa gggtataaag gaagtaagac
      301 aagatgtagc taaatctcta ggagcgaaag aaaaggagat ttattttaca tcaggtggta
      361 cagagtgcaa taatactata ataagaggta tcactagtct aaataaaaaa agaaaaaatc
      421 acataatatc tactaacata gaacatcctt ctgtgttaaa tacattaaaa gatttagaag
      481 aagatggttt tgaagtaact tatttagagg tcggaaaaga tggaaaaata aatatagagg
      541 atttaaaaag tgcgataaag tcaactacat gcttagtaag tatgatgcat gttaacaatg
      601 aaatagggac tatacagcca ataggagaag tcggaaagta tttgaaaggt ttaaaagaaa
      661 agatttattt ccatgttgat gctattcagt catatggaaa aattaacttt agaccatcta
      721 aatataatat agattttatg agtgtaagtg cacataaatt ccatggccca aaaggaattg
      781 gatttatgta tataaaagaa aataatcgcc ttaagcctat gttaacaggt ggtggtcagg
      841 aaattggaat taggtcggga acagaaaatg tacctgggat atatggtctt ggagaagctg
      901 ttagaatact gaataaagat ttagatgctg tcatttcgaa agtaaataac ttaaaaaata
      961 cattaaaaaa tgaaataatt gataatatag aagatataaa aattaattct cctgaagatg
     1021 gagtatgtca tatattaaat gtatcattta gagggacaaa aggagaagtt ttacttcatt
     1081 acttagagca aaaaggtatt tatgtatcaa ctggctctgc atgctcttct aaaaagaagg
     1141 gtagtcatgt attaaatgct ataggtctaa caaatgaaga aataaatgga acaataagat
     1201 ttagtttatc tgatatgaac acagaagagg aaatgttaga agctgtgaaa gttttgaaag
     1261 aatcaatttg tgacttaagg tcaataatga aaagaaaata aagaatactg tataaatagt
     1321 gagaataaat tagtttaaga ggtgaaatgg tgtataatat attaatagta aagtatggag
     1381 aaattggagt aaaaggaaag aatagatata tatttgagaa taggcttata agaaatataa
     1441 gaaatatgtt aaagccaatt ggcaaattta atgtatataa agagtatgga agaatatatg
     1501 ttgatttaga agattatgat tatgaagaag taattgaaga agttagaaaa gtatttggga
     1561 tagttggtgt atgtcctgga gtaagagcta aaaaagatta tgatacatta aaagaaatag
     1621 ctttaaaaat gttagaagaa aaaatagatg ctggatataa aacttttaag gtagagtcaa
     1681 gaagaggaga taagtctttt gggttgactt ctcaagaaat gagtatggat ataggtggat
     1741 acttattatc aaaggtagga gataagatta atgttgatgt aagaaatcca gaagttaaaa
     1801 taaaatgtga atacagagaa tttcatacta tggtctatag tgatactata cctggatatg
     1861 gtggattgcc acttggaacg aatggtagag ctatgtctct tttatcagga ggtatagata
     1921 gtccagtagc tacatggatg gttgcaaaaa gaggtatgga ggtagaggca gttcattttc
     1981 atagttatcc atttacaagt gaaaggtcac aggaaaaagt taaggattta gctaaaatac
     2041 tggcaaaata ctgtggtaga gtaagacttc ataaagttaa tatattagaa atacaaaaag
     2101 caattggtga gaattgcaat gaagaagaag ctactattct ttcaagaaga tttatgatga
     2161 gaatagcaca gagactttct gaaaaaagac attgtgatgc tttaattaca ggtgaaagca
     2221 ttgggcaggt tgcatctcaa actatacaag gtcttacttg tactaatgcc gttgtagatt
     2281 taccagtgtt tagaccactt atagccatgg ataaatcaga tatagttgat atagcaaaaa
     2341 aaataggaac ttttgaaaca tctatagtgc ctgaagaaga ttgttgtagt gtattttctc
     2401 ctagaaagcc tgttactaaa cctagattag agaaaataga aaaatctgaa acagcacttg
     2461 atattgaaaa actagtacag gatgcgattg acaaaataga ggtagaggat attgagtttt
     2521 agtacaaaag ttatttaatt attaaattga atattgagca tattggtagt tctgttgaaa
     2581 ggacttatat agacgattta ttaatatgta gtatttaaaa ataagtacta taattttatt
     2641 taatgggaat gattattatg aataaaaagg gttttacact aattgaattg ttggtagtta
     2701 tatctataat aggaatttta gttatagtag ctgttccagc gttatttaga aatatagaaa
     2761 aaagtaaggc ggttacatgc ctttctaata gagaaaatat aaagactcaa attgttattg
     2821 caatggctga ggaaccaagt aaagataaga ataaagtcat aaaagatgta ctagaaaata
     2881 aagatggtaa gtactttgaa acagaaccaa agtgtaaatc aggtggaata tattcagcaa
     2941 cgtttgatga tggttatgat ggaataactg gaggagaaag cattgcaaaa gtgtatgtta
     3001 cttgtacaga acatccagac ggtgttgaaa tggctaggga tgtacatcaa agtatgaaag
     3061 atttgattgc atcatttgca caagaccctt ctataatacc aggagcttca aagagtaatg
     3121 atgattttag aaaatattta ttagacaata aatataaaaa gggatggcct acaattccag
     3181 atgaatttaa agcaaaatat ggattaagta aggatacact atatatacaa ccatatgcat
     3241 ataatcctac taaacctgat gccactgtag ttgtatttgc aaataataag actggaggta
     3301 attggtacac ttccctagtt tacgattatg atgaaggtag atggtataaa ggcaaaaatg
     3361 gtatttctgt tgcaggtagg tcatgggatg ttgatacaga tagtgttaag tctgtaaaaa
     3421 cagagattca ttctaaagag ggatggggtc ctttaaatta atatataatg ttttattaat
     3481 ctggtattga ttaaactata gaaaagagca gtttctatag atgacgaata ccagattttt
     3541 atttatatgt ataattaata tatattaaaa gtatttatat ttttaagtct tcacatgtaa
     3601 gctaaaaata attataataa tgttgtaaag aagtttaact acaagattaa ttttctgaag
     3661 atttagtatt tatactaaga aatacacaaa aaggggaata gatatgatta ataattttca
     3721 agacaaccaa acaataaatt taatacaaag tcgtagaagt atacgaaaat ttacaacaga
     3781 acaaattagt gatgagcaag taaacacatt attacactgt gcatttgcag caccttcagg
     3841 atgtaataaa cagccttggc acataactgt tgtgcaagac cagaaacttt taaaagcgat
     3901 tagtgatgat actttatcta ggattcatga ggtgagtaat gttgagataa ataaaaactt
     3961 taagttattt tatggtgctc caactgtttt atttatttct tatgatgaaa gtagctcatg
     4021 ggctccatat gatgtaggaa tacttacagg gaatataaca acagcagcac aagcactagg
     4081 gcttggaagt tgtataatag gtatgattag aggattattt acacctgttg aacaaggaga
     4141 tattgaaggt cttgtgagtg tattggataa agaagatgta aaagaatcag agtcaataaa
     4201 gatgaagttt gatacaaata aaaaatacag agagatactt gatataccag aagggtattc
     4261 tgtaccattt ggtattgcta taggtattcc agatgggaat ttaccaaatg ctagagaggt
     4321 agtttataaa gtttctagag tttaggataa agtattgcaa caatcaatcg gttatttgaa
     4381 gaattaaatc ggtaatatcc aaataaacta atagaagaga aagctattta aaaagtagct
     4441 tagatttttt attgtaaaat aattaaataa agaatagggt gataagtatt ttatttatta
     4501 tcctattctt ttttatttgt agaatgataa aaaaatgata aaaacaagaa ttaagtgtaa
     4561 tacaatgtaa ataaagtgtg ttaattttga gtatatgttg taaagtttat caaaactgaa
     4621 aaactcaaga tatattttca gtataatagt gtataaaaat agggtaaagt ggaagtctcg
     4681 aagtctctta aaaatttgca cttttcccta ttttattatg ttttctataa aggttaatat
     4741 acactaacta ttatatactt tcactccaaa tttagagatt atttcactag ctttttgaac
     4801 atcttcctta gtggcatcag gaacatcttt tagttcataa tcgataccta aattttccca
     4861 tttgtgtatt cccatagaat ggtatggtag caattcaaat ctttctacat ttttcaatgt
     4921 agctacaaat ttacctagtt tttctaaatt atctacatca tcagtaatac caggaactaa
     4981 aacatgtctt atccatacag gaatatttct tgattctaag tgtctagcaa gttttaatgc
     5041 cttatccata ccaacaccag tcaaatcaat agatttttcc tcaaccatat gttttaaatc
     5101 aagtaaaacc aaatcagtat tgtctaaaac tgggtctata gtttctatat ctacgaatcc
     5161 agatgtatcc aagcaagtgt gtatatcatt ttcttttgct tttttaaata attcacttaa
     5221 aaactctggt tgaagagtgg attcaccacc agaggcagtt atacctccat cagaaaattt
     5281 cataaaagaa gtatacttaa gagcatccgt tattatttca tcaactgtat attcttttcc
     5341 tgatttaata tcccatgtat ccctgttatg acagtatttg catctaagag gacacccctg
     5401 aaaaaataat atatatctta ttcctggtcc atctacagta ccaaacgttt ctatagaatg
     5461 tatttttccc ttaatcatag agttcacctc acaatatatt ttaataacct tatcaatagt
     5521 tataatattt ctctaaaaaa ggccatctta taccagaaat aattcaaata taagatagcc
     5581 ttttattttt attttctaca cagtagtaat cttagttaaa gtataaaaat ttaaagacta
     5641 agccatttta ccgtggaatg ttctgtttat aacatctaat tgttgctctt tagttaattt
     5701 tatgaagtta acagcatatc cagatactct tatagttaat tgtggatatt cttctggatg
     5761 ttccatagca tcttctaaag tagctctatc aaatacattt acatttaggt gatgagcatt
     5821 ttgagcgaag tatccatcca tcattgcaga aagattgttt attctttcag tcatatcttt
     5881 tcctaaagca gcaggtacta tagagaaagt attagatata ccatcttgag cgtgctcata
     5941 tggtaattta gcaactgaag ataatgaagc taaagcacca ttagtatctc taccatgcat
     6001 tgggttagca ccaggagcaa atggagcacc agctcttcta ccatctggtg tattaccagt
     6061 tttcttacca taaacaacat ttgaagttat tgtaagtata gattgagttg gataagaatt
     6121 tctataagtt ttattttttc ttattttatt cataaagctt tcaactaact ctacagctat
     6181 actatcaact ctatcatcat tgtttccgta ttttggataa tctccttcta tttcaaaatc
     6241 aacagctatt ccttgttcat ttctaatagt tttaacctta gcatgtttta tagcagataa
     6301 tgagtctgca caaactgata atccagccat accacaagcc atagttctaa atatatctct
     6361 atcatgtaat gccatttcta atgcttcata agaatattta tcatgcatat aatgtattac
     6421 atttaaagta tttacatata atgttgctaa ccaatcagtg aatggttcaa atttactcat
     6481 aacttcatca taatctaagt actcagaagt tataggttca aatcttggtc caacttgaac
     6541 gccagatttc tcatctacac caccatttat agcatataat aaagttttag ccaagttaac
     6601 tctagctcca aagaactgca tttgtttacc aattctcatt gcagatacac aacaagctat
     6661 accataatca tctccccagt atgctctcat taaatcatca ttttcatact gtactgaact
     6721 tgtatctata gatactttag aacagaaatc tttgaatcct tgaggtaatt tagttgacca
     6781 tagaactgtt aagtttggtt ctggagaagg tcctaatgta tatagagtat ttaacattct
     6841 aaatgagttt ttagtaacta atgttctacc atctattccc atacctccta tacactcagt
     6901 tacccaagtt gggtctccag agaataggtt attgtaatca ggagttctta ggaacttaac
     6961 cattcttaat ttcataacaa aatggtccat atactcttgt aattcttctt ctgttacgat
     7021 tccagctttt aaatctcttt caaagtatat atctaagaaa gtagatgttc tacctaaaga
     7081 cattgcagca ccattttggt ctttaacagc acctaaataa ccaaagtata accattgtac
     7141 agcttctttt gagttagttg ctggttttga tatatcaaat ccataagatt cagccatttt
     7201 tttaagttca tttaaagctg atatttgctc agttatttct tctcttaatc tgataacttc
     7261 ctcatccata caagaaactt ctaatgattt cttttgctca tttttatctt ctattaaagc
     7321 atcaacaccg tatagagcaa ctcttctgta gtcaccaatt attctacctc taccataagc
     7381 atctggaaga cctgttatta taccagattt tctagcagct ctcatttctg gagtataaac
     7441 atcaaaaact ccttggttgt gtgtttttct gtatttagta aatatatctt ttatttctgg
     7501 gtctaattca tatccaaaag cttcgcatga attttcaacc atcttgatac caccatatgg
     7561 catgacagct ctttttaaag gggcatccgt ttgtaaccca acgattgttt ctaattcttt
     7621 atctaaatat cctggagcat atgcagctat tccagaaaca gtttttgtat caacatctaa
     7681 agtaccacca ttttctcttt cttttttaaa tagaaccatt gctttgtccc ataatttttt
     7741 agtattttca gtagcaccag ctaagaaaga gtcatttccc tcatatggag aatagttcaa
     7801 ttgtataaac tctctaacat ttatttcttt agtccatctt cctgttttaa atccttgcca
     7861 tgcattcatt aagtatttcc tccttagatt taaatgataa ataatactat taaaattaat
     7921 ttttatatac ttaatttatt ttatatattt tttaaaaaat atattttcta atataataat
     7981 aacatattat tcgtatcctg tatacataaa aaataaaaaa ttgtcgcttt aaagtataaa
     8041 acagattata tgttaataat atggtgaaat tatacccata tacaaataaa ataaaacaaa
     8101 taaatagcta attaaggaga aaatattttt tattttttgt ttagaataaa taaattttat
     8161 tatggaaaaa agaaaaagca tacatcttca caaactgaat atttatttaa tagggggagg
     8221 tgatatattg tttatcacag tggtattatt tttagtaggt ttcctattaa ttactaaggg
     8281 cgcagatatc tttataaatt gtacggtaga gataggtaga aaaacgaata tatcagaaat
     8341 tatacttgga gctactatag ttagttttgc aacaacatta cctgaattta cagtgtctct
     8401 acttgcttct attgatggtc atactactat gagtttagga aatgcagttg gttctataat
     8461 atgtaataca gggttagcat tagggttggt tgtttttcta agtccattta atgtagataa
     8521 aaaaatgttt ttttctaagt ctttactatt aatagtatcc gttatagttt taattttatt
     8581 gagtttagat ggagttataa caagaggtga ttcgttatta cttataataa tattaatctt
     8641 ttatatggtt aataattata gaagtgtcgt tggaaaatct gatactaaaa gtagaaatat
     8701 taaaaataac actattaaag acatatctac taaaaaaggg aaaaattata gaggtttttc
     8761 cgtattagaa attgtaaaaa tactattact ttttgctacg ggattgataa tgatgataat
     8821 aggttctcaa atccttatag agagtggggt aagaattgct agctttttaa atatacctca
     8881 aggtatagta agtttgacta taatagcact tggaacttct cttccagaaa tagtttcatc
     8941 aattacagct ataagaaaaa atcatcatga aatatcagta ggaaatattt tgggggcaaa
     9001 tatattgaat atagtgtcag taatagctat atcagcaata ccaaataata taccgatact
     9061 atctcaaaat agacaattag atataccatt catgatatta ttgatattaa tagtaataat
     9121 accaacactt aaatcaaata gacttagtag aatacaaggt atattgatgt tattcacata
     9181 ttttctgtac atatcaattt tgtatttcat gtatattatt taactatact gttaaaaata
     9241 ttaattgcag aatattctaa aaaagtatat aatggatagg ttaagattaa ttgataacgg
     9301 agaggaataa tatatgaata taacaaagtt tgaagattta ccaataagtg agggaataaa
     9361 aaaagcaata gctgaaatgg ggtttgaaga accatctcct atacaagcac agtctatacc
     9421 tgcgattttg tcaggtaaag acgttatagg acaggctcaa actggtacgg gaaaaacagc
     9481 tgcttttagt atacctatac tagaaacaat agacccaaat aatagaagtt tacaagctgt
     9541 agtactttgt ccaacaagag agcttgcaat acaagtatca acagaaataa gaaaactagc
     9601 taaatattcg cacggaataa agacactacc tatatatggt ggtcagccaa tagatagaca
     9661 aataaagtct ctaaagagtg gtgtgcaagt tgtaataggt actccaggac gtacaataga
     9721 ccatattaat cgtaaaacat taaaaatgga taacgtaaaa atgattatat tagatgaagc
     9781 agacgaaatg ctagatatgg gatttagaga agatatagaa atgattttaa gtaagattcc
     9841 agaagaaaga caaacaactt tcttttcagc tactatgcca agaggaattt tggaattaac
     9901 taagagatat caaaaagacc cagagcatat aaaagttgtt agaaaagaac ttacagtttc
     9961 aaatacaaag caatactata tagaaactag gtcatcaaat aaattagaag tattatgtag
    10021 attagttgat gtatatgacc ctaaattatc tgttgttttc tgtaacacta agagaaaagc
    10081 agatgaatta gtaggagact tacaagcaag aggatatttt gcagatgcat tacatggtga
    10141 tttaaagcag actcaaagag atatagtaat ggataagttt agaaatggta caatagatat
    10201 acttgttgct actgatgttg cagctagagg tatagatgtt gatgatgttg aatgtgtatt
    10261 caattatgat ttaccacaag atgaagagta ttatgttcat agaatcggta gaactggtcg
    10321 tgctggtaga gaaggtatgt catttacttt tgtatttgga aaagaaatga gaaagatgaa
    10381 agacatagaa agatacacta aatctaaact gataaagcat aatataccaa cgataactga
    10441 tgttgaagag aaaaaagtag gtactttctt tgcacaagtt aaacagacta tcgaagaagg
    10501 gcatttaact aaacagctac agtggttaga aaatttctgt aatgatgaag attatgcaat
    10561 ggttgatata gctgctgcac ttgtaaaact atctctaggt gaagaaatga aagaagagat
    10621 aatagaagaa aaacctagaa gagagcgtgg agatagaaaa ggtggtactg gagctaaaga
    10681 tggtatgatt agattattta tcaatattgg tagaaatcaa agagtacaag caaaagatat
    10741 agttggtgct atagctggag aagttggaat cccaggtaaa gtagtaggca ctatagatat
    10801 atatgataag tatacttttg ttgaaatacc taaaaaagat gcaaaaacag tgattgaaaa
    10861 gatgaaagat attaagataa aaggtaataa gattaatata gaaaaagcaa ataaaaagaa
    10921 aaaataacgt atagtataca aaactataat tagaaaatat ataaaataat atattgaata
    10981 tttaaaaagc ctgatgtgtt ttcaaaacag atagtagata atctgtaata gttaacagca
    11041 taaggctttt attgttttat ataatattat tgttaggagt gtattaaaat tagttt
//
LOCUS       DAFNNB010000127        11083 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0128, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000127 DAFNNB010000000
VERSION     DAFNNB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 11083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11083
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0128"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..591)
                     /locus_tag="L3M60_002767"
     CDS             complement(<1..591)
                     /locus_tag="L3M60_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227410.1"
                     /translation="MESRGYKEDLKKMILDMAHKELESYVSQKGAKKYFEGNLDKIIN
                     NNIKKFNQSIGLNRVYLKMLKKCAYQESVTSISRTIKKEALFKSKKELFAFSEYLKLD
                     INKRLSYNQILRKISRYIYYNRSSYAQKYVVFKRGDEEYLLEPEKIKDELISSYRSKT
                     REDMRSIAKLLDIEVEDDYNAEDIRKKVINYIIKEKL"
     gene            complement(792..1004)
                     /locus_tag="L3M60_002768"
     CDS             complement(792..1004)
                     /locus_tag="L3M60_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HBN6227411.1"
                     /translation="MKKKLLVEGMSCGHCVNHLKTALTEDIDGIEVLDVDLENKCASV
                     DMKDSVSIEKLKEVIAELGFELKGIE"
     gene            complement(1077..2792)
                     /locus_tag="L3M60_002769"
     CDS             complement(1077..2792)
                     /locus_tag="L3M60_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6227412.1"
                     /translation="MKRIKVLTVVLAITLMVVGCSNSKTKQSSSDSSLQSQSSIDAKY
                     INLTMVTPKTINPITNTDKSVGYIMNLVYDSLFTIDENYNVIPQLVKEYNIAQDGMSI
                     DIKLKDAKWHDGKDVTSNDVNYTIGLIQKSADSPYNEFTKNIASVNIKSDKDFTIKFK
                     ARYAFSIDSLIFPIVSQSHLDSKDVNDKNNNMIGNGKYKIESYTEREGMVLSVNKDYY
                     EEVPKTMKNIKVGMVPNEDARTSMVMALDSDITNVTLNDLSKFQEKEFNITKYQGRDY
                     ECVLFNYNNPFFKDVNFRKAIAHSIDKDRIISEGYMDDATPVNFPLNSKSKYYNSEMK
                     DLEFNKDKALECLAKVEYANVNPVNQNDNKIKNQKKPAKKNLKKLTPEEEAKAKKAEE
                     DSIKKEAEEKQNREKEEVKKKLSEMNLKIIVNRDNSERIKTANIINENLKAIGINSTV
                     NQLSDKDMENALNSKDYDLALVGWKLSSIPDASSIIASSGYTDDKLNGYMSALTSSTS
                     EIETKKAYKDVQTYIKDNATFISLAVRNNYIVSSSRLKGKITPNDFDVYEGISNLDIK
                     SNESN"
     gene            complement(2792..3346)
                     /locus_tag="L3M60_002770"
     CDS             complement(2792..3346)
                     /locus_tag="L3M60_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBN6227413.1"
                     /translation="MNSKKVLILSIFIILFGALLMESKVIHKFLYPKKYSEYVEKYSK
                     EFNLDENIVYSVIKAESKFNSSAVSKKEAKGLMQILDITRDWGAEELNLKNVDIFDPE
                     TNIRLGCWYLSKLYKEFGKLDLVIAAYNGGSGNVKKWLENNEYSKDGENLHDIPFKQT
                     SKYVEKVKNNYEQYNKIYGKKGKN"
     gene            complement(3339..3941)
                     /locus_tag="L3M60_002771"
     CDS             complement(3339..3941)
                     /locus_tag="L3M60_002771"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBN6227414.1"
                     /translation="MLILGLTGGIGCGKSSLSNIFRNLNISIVDADIISRKIFEDKLL
                     LEKVFVHFGQSIKNDDGTLNRKALGKIVFSDEEKLKKLNNLTHPRIREKIISEIEELR
                     KKGENIVVLDAAILVESGFLDMVDKLLVVTCKQEVQISRIQKRDNCSEQEALNRINSQ
                     MSQEEKSKYGDYIIDNSGTITELESKAHKFIEYMKENWRE"
     gene            complement(3950..6598)
                     /gene="polA"
                     /locus_tag="L3M60_002772"
     CDS             complement(3950..6598)
                     /gene="polA"
                     /locus_tag="L3M60_002772"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBN6227415.1"
                     /translation="MEKKLIIIDGNSIINRAFYALPEMNNKEGLKTNAIYGFTTMLFK
                     MIDIYKPTHISVAFDRKAPTFRHLEYKEYKAGRKGMPDELAEQLQPLKDLLDKFKINR
                     LEIDGYEADDIIGTVSKKAEDDGYKVYIVTGDKDAIQLASDNTTTLITKKGVGEVEEY
                     NFNSVIEKYEMTPTQFIDLKGLMGDKSDNIPGVPGIGEKTGIKLIKEFSSIENLLENT
                     DKLKGSVKKKIEENKEIAIQSKRLATIIRDVPIEVDLESMIFGDYDKDELLDKFRYFG
                     FTSLLSRLVDLVDSNDTEQVEEKIEILKLKDIEKFIDEVNEKGQVILKTVRGQGNILE
                     KNIIYIFISVDGEKIYYVDSNEVSKLDSIFSNNEIKKYGYNLKDDYISLKPYNINLEN
                     MYFDITISEYLIDSTSSTSYDCSAIAMKYLSKKVKSKEELLGKGVKSKNYEDLEFEDL
                     AEYMGQIICTVKETVPMMERSLMDMSMDGLFYHVEMPLVEVLGHMEYEGIKVDKSILD
                     ELSVEFKEIIATLEKEIYELSGEPFNINSPKQLGVILFEKLELPIIKKTKTGYSTNAD
                     VLEKLRDKHPIIDKITEYRQIVKLNSTYVEGLLNIINPESNRIHSSFNQTITTTGRIS
                     STEPNLQNIPIKMEMGRKIRKVFIADDNCKLVDADYSQVELRVLAHMSQDENMIEAFK
                     HHEDIHTKTASQVFNVPMEDVTSELRGAAKAVNFGIIYGKSDFGLADDLNIPVPKAKE
                     YIESYFAKYHKIKEFMDTTVEKASEDGYAVTILNRRRYIPEIKSSNFMERNRGKRFAM
                     NAPIQGSAADIIKIAMINVHKKLEENNLKSKLILQVHDELIVEAIDDEIDIVKKIVKE
                     EMENAVNIDVHLDVDLNVGSSWYETK"
     gene            6917..6999
                     /locus_tag="L3M60_002773"
     tRNA            6917..6999
                     /locus_tag="L3M60_002773"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6951..6953,aa:Leu,seq:caa)
     gene            7690..9099
                     /locus_tag="L3M60_002774"
     CDS             7690..9099
                     /locus_tag="L3M60_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBN6227416.1"
                     /translation="MIFSSLIFLFLFFPVTLICYYLANPKYKNVMLLLASIAFYTWGE
                     PKYIFLMILSIIVNYIFGIKVSGSKNKKMWVVLSVIYNISILAVFKYSNFFIDNINFL
                     FNTDITIPEVKLPLGISFFTFQIMSYVIDVYRNDAKVQRKLANLALYISLFPQLVAGP
                     IVRYQTVADSIEYREHNWDKFTQGVNRFVVGLGKKVILANQLGVIADGVFNKSAGDLS
                     RFEVWLGIVCYTLQIFYDFSGYSDMAIGLGKMFGFEFLENFNYPYISQTVSEFWRRWH
                     ISLGTWFKDYVYFPLGGNRVSKAKLYRNLFVVWFLTGMWHGTSWTFIILGLYYGILIS
                     VEKAFLEKFLYKMPRIVRHIYLLLIVMIGWVFFRVDDLPAAIKFIGIMFGAGNYPVIS
                     NAFNVFIIEYWYVIVGAILLSTPIVKVLKSLLIKKNKNTLNSTLYQVTNMVFIVSCMF
                     IVIIMLSSSTYNPFLYFRF"
     gene            9115..10308
                     /locus_tag="L3M60_002775"
     CDS             9115..10308
                     /locus_tag="L3M60_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227417.1"
                     /translation="MSKKKPRIVAISFLIIVFGFFILNFTLPKKKLSITENRNLAKFP
                     TVENLKKGDFIAKFEEYFNDHFAFRDRMISINTKAQASLNKTKVGNYYLGKDNWILGM
                     FPEILDQKQLSRYEKSINYLSNLSKDMGKNVYFTMMPHKTNMLKHLYPKYVDNMDNID
                     INLKNFKNRLNPDLIKYIDMDEYFLSNYSDKERENLYFKTDHHWTGYGAFEGFKMMAS
                     NMDLGLSKQDLDKHFSSYKKSIITDKKFIGSYNQNIDMLIKENEYVVYMTKDNQDYSF
                     DINGEEKEEKEVYATERDNKEWDYGGAYIRGAKTNILTIKNPKALVNKKILIFRDSYQ
                     APTTLLFADMFKEVEIADPRNINSIYKTYKEIIEESNADIVMFMYNSSGFDSMIDSMI
                     DKGIK"
     gene            10425..10805
                     /locus_tag="L3M60_002776"
     CDS             10425..10805
                     /locus_tag="L3M60_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227418.1"
                     /translation="MSKLNRKKDFYFVSLPHKTNMLKNLYPSGIKGKNNIDINKENLE
                     KQLKDINFLDIDDYFYKSFTEKERENFFLKTDHHGNSLGAFEEYKFILDKLDLSLSKS
                     EIDSHLKRYSTRYVNDCECSFIYV"
BASE COUNT         3639 a         1702 c         1273 g         4469 t
ORIGIN      
        1 aagtttttct ttgatgatat aatttattac tttttttcta atatcttcag cattataatc
       61 atcttctact tctatatcca aaagttttgc tattgacctc atatcttctc tagtctttga
      121 cctatagctt gatattaatt catcttttat tttctcaggc tctaaaagat attcttcatc
      181 tcctcttttg aaaacaacgt atttttgagc atatgaactt ctattgtagt atatatatct
      241 tgatattttt cttaaaattt gattatatga aagtcgcttg ttaatatcaa gttttaagta
      301 ctctgaaaaa gcaaatagct ctttttttga tttaaataat gcttctttct ttatagtcct
      361 tgatattgaa gttacacttt cttgatatgc acactttttt aacattttta aatatactct
      421 attaagaccg atactttgat tgaatttttt tatattatta ttaataatct tatccaagtt
      481 accttcaaag tatttttttg ctcctttttg tgatacataa ctctcaagtt ctttgtgggc
      541 catatcaagt atcatttttt ttaaatcctc tttatatcct ctagattcca tgatactccc
      601 tcccttagct atttatagta tattcaaaaa gctcaagaaa tgtgagtcat tacaaatttt
      661 ttttactttt tatgtatata ttaatttaaa aggggagata atagtaatgt aaaatcaaac
      721 atactcaatc tcccccattt ttagacctct attactcccc caatagaggt ctttttttag
      781 tttattaact tctattcaat tccctttagt tcaaatccta attctgctat tacttctttt
      841 aatttttcaa tagatacact atctttcata tctacagatg cacacttgtt ttctaaatct
      901 acatctaaaa cttcaatacc atctatatct tctgttaatg cagttttaag atgatttaca
      961 caatgaccac aactcatacc ttctactaat agtttcttct tcatttaatt acctccttat
     1021 accctataag ggtattttat catatagttt atacttttaa aatagaaatt aatcatttaa
     1081 tttgattcat tcgattttat atctagatta gatattccct catatacatc aaaatcattt
     1141 ggagtaattt taccttttaa tctactacta cttacaatat agttgtttct aacagctaaa
     1201 cttataaatg ttgcattatc ttttatatat gtctgtacat ctttgtatgc tttttttgtt
     1261 tcaatttcag atgtagaact tgtaagtgca gacatatatc catttagctt atcatcagta
     1321 tatccactac tagctataat actacttgca tctggtatac ttgataattt ccatccaact
     1381 aatgctaaat cataatcttt agaattaaga gcattttcca tgtccttatc tgacagctga
     1441 tttactgtac tatttattcc tatagccttt aagttttcat ttattatgtt tgcagttttt
     1501 attctctcac tattatctct gtttactatt atttttaagt tcatctcaga aagttttttc
     1561 tttacttcct ctttttctct attttgtttc tcttcagctt cttttttgat gctatcttct
     1621 tccgcttttt tagcttttgc ttcttcttct ggagtaagct tctttaaatt ctttttagct
     1681 ggttttttct gattctttat tttattgtca ttttgattta ctggatttac atttgcatat
     1741 tctaccttag caaggcactc tagtgcttta tctttattaa actctaaatc cttcatttca
     1801 ctattatagt attttgattt tgaattaagt ggaaaattaa ctggagtagc atcatccata
     1861 taaccttcac taataattct atctttatct atagagtgcg caattgcttt tctaaaattg
     1921 acatctttaa aaaaaggatt attataatta aataatacac actcataatc tctaccttga
     1981 tattttgtta tattaaactc tttttcttga aacttactta aatcatttaa tgtaacattg
     2041 gttatatcac tatcgagagc cataaccata gatgtccttg catcttcatt aggcaccata
     2101 cctactttta tgttcttcat ggtctttgga acttcttcat agtagtcttt gtttacagat
     2161 aaaaccattc cttctctttc tgtatatgat tctattttat atttcccatt tcctatcata
     2221 ttattattct tgtcatttac atcttttgaa tctaaatgac tttgagatac tattggaaaa
     2281 attaaactat caattgagaa agcatatcta gctttaaatt ttattgtaaa atctttatct
     2341 gatttaatat tcacagaagc tatatttttg gtaaattcat tatatggact atctgcactt
     2401 ttttgaatta aaccaatagt atagtttaca tcatttgaag taacatcttt tccatcatgc
     2461 cactttgcat ctttcagctt tatatctata ctcattccat cctgagctat attatactcc
     2521 ttaactaatt gaggaattac attgtagttc tcatctatag taaataaact atcataaact
     2581 aaattcatta tataaccaac agacttatca gtattagtta taggatttat agtttttgga
     2641 gttaccattg ttaagtttat atacttagca tctatagaac tttgactttg taaacttgaa
     2701 tcactactac tttgttttgt tttactatta ctacaaccaa ccaccataag agttatagct
     2761 aaaacaactg ttaagacttt tattctcttc attagttttt tcctttcttg ccatatatct
     2821 tattatattg ttcataatta ttttttactt tttctacata ttttgaagtt tgcttaaaag
     2881 gtatatcatg tagattttcg ccatctttac tatactcatt attttctaac cattttttca
     2941 cattacctga accaccatta tatgcagcta tcactaaatc taatttacca aattctttgt
     3001 ataacttact taaataccaa caaccaagtc ttatattagt ctctgggtcg aaaatatcca
     3061 cattttttag atttaattcc tctgctcccc aatctctagt tatgtctaat atttgcatta
     3121 atccttttgc ttcttttttt gaaacagcag aactattaaa cttactttcg gctttaataa
     3181 cactgtaaac tatattttca tctaaattaa attctttaga atacttttct acatattctg
     3241 aatatttttt aggatataaa aatttatgta ttactttgct ttccattaat agtgctccaa
     3301 ataagattat aaaaatagat agaatcaata ctttcttact attcacgcca attctccttc
     3361 atgtactcaa taaatttatg tgctttactt tctaattcag ttattgttcc cgaattatct
     3421 attatataat caccatattt ggatttttct tcctgactca tctgtgaatt tatcctatta
     3481 agtgcttcct gctcactgca attatctctc ttttgtattc tactaatttg tacttcttgt
     3541 ttacaagtaa ctactagtag cttgtcaacc atatctaaaa aaccactttc taccaatatt
     3601 gccgcatcta atactactat attttcgcct ttttttctca attcttctat ttcacttatt
     3661 attttttctc ttattcgtgg atgtgtcaaa ttatttaact tcttaagttt ttcttcatca
     3721 ctaaaaacta ttttcccaag agcttttcta tttagagttc catcatcatt ttttatactt
     3781 tgcccaaaat ggacaaatac tttttctaat aacaacttat cctcaaatat cttcctggat
     3841 ataatatcag catccactat agaaatgtta agatttctaa atatatttga taggctgctt
     3901 tttccacagc ctattcctcc tgtaagtcct aatatcaaca taaaatcacc tactttgtct
     3961 cataccaaga actgcctaca tttaaatcta catctaagtg aacatctata tttacagcat
     4021 tttccatttc ttcttttact attttcttaa ctatatctat ttcatcatca attgcttcaa
     4081 ctatcaattc gtcgtgaact tgtagtataa gttttgattt caaattgttc tcttccaatt
     4141 ttttatgaac atttatcata gcaattttta ttatatctgc tgcacttcct tgtattggtg
     4201 cattcattgc aaatctcttt cctctatttc tttccataaa attgcttgac tttatttctg
     4261 gtatatatct tcttctgttt aaaattgtta cagcatatcc atcttcacta gccttttcta
     4321 ctgttgtgtc cataaactct tttattttat ggtatttagc aaaataactt tcgatatatt
     4381 ctttcgcctt tggaacaggt atatttaaat catcagctaa gccaaagtca cttttaccat
     4441 atataatacc aaagtttact gctttagctg cacctctcaa ttcactagtg acatcttcca
     4501 taggaacatt aaaaacttgt gaagctgttt tagtatgaat atcttcatga tgtttaaatg
     4561 cttctatcat attttcatcc tgactcatat gtgcaagaac tcttagctct acttgtgagt
     4621 aatcggcatc aaccaactta cagttatcat ctgcaataaa cacttttctt atttttctac
     4681 ccatttccat ttttataggt atattttgaa ggtttggttc tgtagatgaa attcttcctg
     4741 tagtcgttat agtttgatta aatgatgaat gtattctatt actttctgga tttattatat
     4801 ttaaaagtcc ttcaacgtaa gtagaattta attttactat ttgtctatac tcagttatct
     4861 tatctattat tggatgctta tctcttaatt tttctagaac atctgcatta gttgaatatc
     4921 cagttttagt ttttttaatt attggaagct ctaatttttc aaataatatg acacccaatt
     4981 gttttggtga atttatattg aatggctctc ctgataattc ataaatttcc ttttctaatg
     5041 tagctattat ttctttaaac tctacactca attcatccaa tatacttttg tctactttta
     5101 taccttcata ctccatatgt cctaatactt ctactaaagg catctccaca tgataaaaaa
     5161 gaccatccat actcatatcc attagacttc tttccatcat aggaactgtt tctttaactg
     5221 tgcatattat ttgacccata tactcagcta aatcctcaaa ttctaaatct tcatagtttt
     5281 tagatttaac accttttcct aataattctt cctttgattt aacctttttt gataaatatt
     5341 tcatagctat tgcactacaa tcatatgaag tagatgaagt tgaatctatt aagtactcag
     5401 atattgttat atcaaaatac atattttcta agtttatatt atatggtttt aaagatatat
     5461 aatcatcttt taaattatat ccatactttt tgatttcatt atttgaaaat atactatcta
     5521 actttgacac ttcatttgaa tctacataat atattttttc tccatctaca cttataaaaa
     5581 tatatattat atttttttca agtatattac cttgtcctct aacagttttt aaaataactt
     5641 gtcctttttc atttacttca tctataaatt tttctatatc ttttaatttt aatatttcta
     5701 ttttttcttc tacttgttct gtatcatttg aatcgaccaa atcaactagc cttgacaaaa
     5761 ggcttgtaaa tccaaaatat ctaaatttat ctaaaagttc atctttatca taatcgccaa
     5821 aaatcatact ttctaaatct acttctattg gcacatctct tataattgtt gcaagtcttt
     5881 tactttgaat agctatttct ttattttcct ctattttctt ttttacactg cctttaagtt
     5941 tatctgtatt ctctaataag ttctctatac tagaaaactc ttttataagc tttatacctg
     6001 ttttctcacc gattccaggt actccaggta tattatccga cttatctccc ataagacctt
     6061 ttaaatcaat aaattgtgtt ggagtcatct cgtatttttc aattacagaa ttaaagttgt
     6121 attcttcaac ttctccaact cctttttttg ttataagagt agttgtatta tctgaagcaa
     6181 gttgaatagc atctttatct ccagttacta tgtatacctt atagccatca tcttctgctt
     6241 ttttagaaac agttcctata atatcatctg cctcatagcc atctatttct aatctattta
     6301 ttttaaattt atctaaaagg tcttttagtg gctgtaattg ttctgccaat tcatctggca
     6361 ttccttttct tccagcctta tattctttat actctaaatg tctaaatgta ggtgcttttc
     6421 tgtcaaaagc tacacttata tgtgttggtt tatatatatc tatcatttta aatagcattg
     6481 ttgtaaaccc atatatcgca ttagtcttta acccttcttt attgttcatt tctggcagag
     6541 cataaaaagc tctgtttatt attgaatttc catctattat aattaatttt ttttccaaat
     6601 tatatcactc ctaaaaatta tctatttttg taacatatat attatatacc aatatagtgt
     6661 tatttaatat tcccataata ataatagtat attaaaatta aatatgttgc aaacaatata
     6721 tttttttgct ataataatat acatgacatg cccgagtggt ggaattggta aatacaatgg
     6781 attcaaaatc aactgtgttg attcataagt ttttatgatg ttttacagtt tgatgagtat
     6841 tttgtattca atattcctct tgcatattct tggaaaatct ggtaggatat acaagtagtt
     6901 cggatttaaa atataagccg ctgtggcgga actggcatac gcatacgact caaaatcgta
     6961 cgggaaacca tatgggttcg agtcccatca gcggcaccaa taaaaaaaac tctttagtat
     7021 atactaaaga gtttttttat tgtaatattc tttcatcttt atttctagtc ttgtataagt
     7081 ataaatacca tagtcataac ttcttgaact cctaaaatat atctaaacaa agtaggattt
     7141 tttatgtatt cacttaataa atccatactt cttatcatat tgattttttc ccactctcta
     7201 aaacttttcc ttctctaaaa atatttataa ccaatcatca ttattcagta taatgattct
     7261 atattatttt aaagtatttg tcatttaaaa caaacttgtg ataaaagcat atctgaaagt
     7321 attctgattc taatacttaa taaagagttc tttttacagt tatagatata aacaacttct
     7381 atcttaatag actgtataga agcacaatca aatgcaaaca catatagtta agtaatatgt
     7441 ccttgatttt tctttattat tttattgtaa tagctttatt aattcattat aatattacat
     7501 catttaaaaa aaattatatg aaaagtcaaa atttacatat agattaatta aacttgtaaa
     7561 aacacatata aattcgtaca attaagtact taaaacttga aaatagttac tttgctatat
     7621 attttagata aagatataat ttatttggtt aagaaaagaa gtggtaaata taaaaataag
     7681 gagtctaata tgatattcag tagtttaata tttttatttt tattctttcc agttacactc
     7741 atttgctatt acttggcaaa tcctaagtat aaaaatgtta tgttacttct agcaagtatt
     7801 gccttctata catggggaga accaaaatat atttttttga tgatactatc aataatagta
     7861 aactatatat ttggtattaa agtttctggc agtaaaaata aaaaaatgtg ggttgtgctt
     7921 tctgttattt acaatatatc tattcttgct gtatttaagt atagcaattt ttttatagat
     7981 aatattaatt ttttattcaa tacagatata actatacctg aggttaaatt acctttaggt
     8041 atctcatttt tcacattcca aataatgagc tatgtaatag atgtatatag aaatgatgct
     8101 aaagttcaaa gaaaattagc taatttagca ttgtacataa gtttattccc tcaacttgta
     8161 gcaggtccta ttgttagata tcagactgtt gcagatagca ttgagtatag agaacacaat
     8221 tgggacaaat ttactcaagg tgtaaacaga tttgttgttg gtcttggaaa aaaagtaatt
     8281 ctagcaaatc aattaggtgt aatagcagat ggtgtattta ataagtcagc tggagattta
     8341 tcaagatttg aggtatggct aggtatagtt tgctatacac ttcaaatatt ctatgacttt
     8401 agtggctata gtgatatggc cattggttta ggtaaaatgt ttggatttga gtttttagaa
     8461 aatttcaact atccatatat ctctcaaaca gtatctgagt tttggagaag atggcatata
     8521 tctttaggta cttggtttaa agactatgta tacttcccat taggtggaaa tagagtctct
     8581 aaggcaaaac tatatagaaa tctatttgta gtatggtttt taaccggcat gtggcatgga
     8641 actagttgga cttttattat attgggtcta tattatggaa tattaatatc agtagaaaaa
     8701 gcattcttag aaaagtttct atataagatg ccaagaattg taagacacat atatctactt
     8761 cttatagtca tgattggatg ggtatttttt agagttgatg atttaccagc agctattaaa
     8821 tttataggta ttatgtttgg tgctggaaat tatccggtta tcagtaatgc attcaatgtg
     8881 tttataattg aatattggta tgtaattgtt ggggcaatcc ttctatcaac tcctattgta
     8941 aaagttttaa aatcattatt aattaagaaa aataaaaata cattaaatag cacattatat
     9001 caagttacta atatggtttt cattgtttct tgtatgttta ttgtaataat aatgttaagt
     9061 agctcaactt ataatccatt cttatatttt agattctaat tgggaggtat aaacatgagt
     9121 aaaaaaaaac caaggattgt tgccatttca tttttaatta tagtatttgg atttttcata
     9181 ctaaacttca cacttccaaa gaaaaaatta agtataactg aaaatagaaa tcttgcaaaa
     9241 tttccgactg tagaaaattt aaaaaaaggt gactttatag ccaagtttga agaatatttt
     9301 aatgaccatt ttgcattcag agatagaatg atatcaataa atacaaaggc tcaagctagt
     9361 ttaaataaaa ctaaggtagg taactattat ctaggaaaag ataactggat attaggtatg
     9421 tttccagaaa ttttagacca aaaacaatta agtcgatacg aaaagtccat taattattta
     9481 tctaatctaa gtaaagatat gggaaaaaat gtgtatttta caatgatgcc acataaaact
     9541 aatatgttaa aacatttgta tcctaaatat gttgataata tggataatat agacataaat
     9601 ttaaagaatt ttaaaaatag attaaatcca gatttaatta aatatataga tatggatgag
     9661 tattttttaa gtaactattc agataaagaa agagaaaatt tatattttaa aactgaccat
     9721 cattggactg gatatggagc atttgaaggc tttaaaatga tggcatcaaa tatggactta
     9781 ggtctatcta agcaagattt agacaagcat tttagtagct ataaaaagtc tataattact
     9841 gataagaaat ttataggaag ttataatcaa aatatagata tgctaattaa agaaaatgaa
     9901 tatgtagttt atatgacaaa agataaccaa gattatagct ttgatataaa tggagaagaa
     9961 aaagaagaaa aagaggttta tgcaactgaa agagataata aagagtggga ttatggtggt
    10021 gcatacatta gaggagctaa aactaatata ttaactataa aaaaccctaa agcattagtg
    10081 aataaaaaaa tactaatatt tagagattca tatcaggctc ctacaacact tttatttgct
    10141 gatatgttta aagaagtaga gatagcagac cctagaaata taaactcaat atataaaaca
    10201 tacaaagaga taatcgagga aagtaatgca gatattgtaa tgtttatgta taatagttct
    10261 gggtttgatt caatgataga ttcaatgata gataaaggaa taaagtagta aataaaaaga
    10321 ttaaacaaaa gtattatcta actaaagatg gatggctatt aggcaacttt gccaaattat
    10381 tatctgatta tcatataaaa tcttactcca aagccattaa taacttgagt aagttaaata
    10441 gaaaaaaaga tttttacttt gtttcacttc ctcataagac aaatatgctt aagaacttgt
    10501 atccaagtgg tataaaaggt aaaaataata tagatattaa caaagaaaac ttagaaaagc
    10561 agttaaaaga cataaatttc ttagatatag atgattactt ttataaaagt tttaccgaaa
    10621 aagaaagaga aaacttcttt ttaaaaacag accatcacgg aaattcatta ggagcatttg
    10681 aagaatataa gtttattctt gataaattag accttagctt atctaaatca gaaatagata
    10741 gtcatttaaa aagatacagt actcgatatg taaatgactg cgaatgtagt tttatttatg
    10801 tataatagct ttggatttgc tggaatgata caagaaatga ttgacaaggg aatagcataa
    10861 caatttgaat caactccttt ttatacaaaa aatagttctc aataattcct aatctatatg
    10921 ataatttagt acatctcttt aacaataaaa ataaaatcac ataaattctt ataccatcac
    10981 ttatctttat gataaattgg ttgagaaaaa attttataat ttatatgata tgtaatctta
    11041 attaattctt tactttttaa ttctttatct actgcaacct ttg
//
LOCUS       DAFNNB010000128        10919 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0130, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000128 DAFNNB010000000
VERSION     DAFNNB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0130"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(80..982)
                     /locus_tag="L3M60_002777"
     CDS             complement(80..982)
                     /locus_tag="L3M60_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6227419.1"
                     /translation="MYNKIKYIPINCEIACNKLKRSMPYKWDLNIYRGCEHGCKYCYA
                     IYSHKYINSNNYFEEIYVKTNIVEKLERQLKSSKWKKEVINIGGVTDSYQPIEADYKI
                     MPEILNLLIKYKTPAIISTKSDLILRDYDLIDKLSRITYINVASTITTVNEDTQRLIE
                     PNGVDSIRRFKMLKEFRKTNASVGLHIMPIIPYITDDFDNINLLFRHAKECNVHYVLP
                     GTLYLRGITRGIFFDFVKKEFPELFDKLSILYSTGSLNRGYKNQLYKMVNELRYKYSL
                     SSSYSKIMKEKLKNSGDVQISFFD"
     gene            complement(1032..1493)
                     /locus_tag="L3M60_002778"
     CDS             complement(1032..1493)
                     /locus_tag="L3M60_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HBN6227420.1"
                     /translation="MRLWHKDLIDVLPKNQLVSQWRELLAIKGSIDKKGTPNHILVNK
                     VLNYSIDEFKFYTKIVHDEMLKRNYKPNELKYTGILKWKNRNFANDISNEHSLDLENL
                     YDGWHNKMYLKQCLYNLEEKATCGGIPINEWNILLCKYSKDYELWYGNIMF"
     gene            complement(1554..2009)
                     /gene="def"
                     /locus_tag="L3M60_002779"
     CDS             complement(1554..2009)
                     /gene="def"
                     /locus_tag="L3M60_002779"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBN6227421.1"
                     /translation="MAIRKIRTFDDEILRKKSKYVENVDNKIREILNDMAETMYNTPN
                     GGGLAACQVGVLKRLVVIDLGEGLIKLVNPEIIKQEGEQIVVEGCLSFPEVWGKLKRP
                     KKVTVQALNEYGEKIEIKGSGFMAKCLCHEIDHLNGIVFTDKIIEHVKL"
     gene            complement(1981..2244)
                     /locus_tag="L3M60_002780"
     CDS             complement(1981..2244)
                     /locus_tag="L3M60_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227422.1"
                     /translation="MKNKIKILREKLGLTQEQLGRLVGTSRQAINAIETGKNEPSIWL
                     AYDISRIFNEPIESVFLFEESERKSRAQISRGEYYGYKKDKNI"
     gene            complement(2341..3057)
                     /locus_tag="L3M60_002781"
     CDS             complement(2341..3057)
                     /locus_tag="L3M60_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227423.1"
                     /translation="MIKIINAFKQEFEQIKRDAMLFIVCVSPILCGVFIKFGIPLIQN
                     ISLNKFYYQLNLEPYLLMFDLLLAFITPFMFFFASTMVVLGEIDDSISRYLIVTPLGK
                     TGYLVSRFGIPGILAFIITMILLIFFSLTRISFLLNLSISLLVLLQSVIISILVISLS
                     SNKLEGMVITKFSGVFMMGILAPFFILNKVQYILFFLPTFWLSKAFKEDNYIYMFISI
                     IISLIWILLLFKKFSKKIVK"
     gene            complement(3054..3737)
                     /locus_tag="L3M60_002782"
     CDS             complement(3054..3737)
                     /locus_tag="L3M60_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227424.1"
                     /translation="MRLKNLVLGDIKFQFKYGFYFLYLFLSIIYICIINVFPTFMREK
                     IAIIMIYSDPAAMGLFFMGAIVLLEKSQRVLNSLAVSPVKVSEYILSKVISLGVISSI
                     VAMLIAITLNLDNIIISTIGTFFSSIIFSLLGLILASKASSLNQFIVLSIPIEIICFI
                     PPILNVLVDTKSYANFYPFNICISLISGDKNFIVINILILISMIIIIYFITYYFICSS
                     WKKVGGVKL"
     gene            complement(3734..4579)
                     /locus_tag="L3M60_002783"
     CDS             complement(3734..4579)
                     /locus_tag="L3M60_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227425.1"
                     /translation="MIKVDDLSFSYTDKNFLQNINFEVGKGEILGFLGPSGAGKSTLQ
                     KILIGMITNYKGSVIVNGVESKRHSNKFYENIGVDFEFPSLYEKLTAIENLKYFGSLY
                     SKKLLSIDKLLKSVGLENKANKRVSEYSKGMKSRLNFIKALLHNPDILFLDEPTSGLD
                     PSNSKVMKDIILSEKSKGKTIILTTHNMLDATELCDRVAFIVNGKISALDTPHNLIMS
                     KGAIKVKYTYFDNGEKTSECFLNNTANDKNLNMLIEKNKLLSIHSSEPTLNDIFIEIT
                     GRNLQ"
     gene            complement(4693..5283)
                     /locus_tag="L3M60_002784"
     CDS             complement(4693..5283)
                     /locus_tag="L3M60_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227426.1"
                     /translation="MPKSYSDKEREYIIKRLKEEARLCMELYGIRKTTVDELVKRVKI
                     PKGTFYLFFQSKELLFFEVLRDIHDSIQKEILYEINRLDKSITCEQLTDIFMKFYRMV
                     DSTSILNLMVNGEFEILVRKLPDAIIEEHFRHDDFEIGEIISCIPNAKNKDIERFSGA
                     FRAVFLTMLYKREVGSNCFEDALRLMINGLVIQLME"
     gene            5573..5806
                     /locus_tag="L3M60_002785"
     CDS             5573..5806
                     /locus_tag="L3M60_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBN6227427.1"
                     /translation="MTTTTKNRFYDYVQENLNKYVEVHSYFSSCPLYGEIIEADSLSI
                     TLCSRYIDESNPTLNETYTLYLPLSSIISIRTL"
     gene            complement(6029..8416)
                     /locus_tag="L3M60_002786"
     CDS             complement(6029..8416)
                     /locus_tag="L3M60_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp13"
                     /protein_id="HBN6227428.1"
                     /translation="MKRFTSKKVARFVALFLISILVMSTIPASADTSNTLNQIKKAEY
                     SETYKQYLNDVKNGRTEKYNGIIPNPYQLEGTQIKSKGRTAPTSYDPRKLGLMTSVKN
                     QENLGICWDFAAMATLESFLKLNNYGDYDLSEEHLRWWASDGEYGWSVNDMDGALNYE
                     AMGYLTSCSGPKLEKDIPYNGKISKAQGATKPTNMNTAPTVFNVTDAVCVSNDINSTK
                     NAILQYGAVTSAYYEDIKYQSDDQNSYYCPTKSFNTNHAISIVGWDDNYSKSNFNSNI
                     RPSKNGAWLIKNSWGDYNSEGGYFWISYEDKTLMSDIDNFSIKGGQKPSDDEKMYQHD
                     YASIVPLISNKITAANVFDFNRGDETLKSVMFLTESIGAKYEVYYAPVVNGIPQENNM
                     KKLKEGTTQYSGYITVPIDSFDIPEGKGAIVVSIEGKNGESTIGSESNVPGYDTFKAK
                     ANLGESYIIDNTGKFFDINRDSKFYPCNFTIKAVTEKSSSESVPNESLIGSDRYETAI
                     KVSQNGFNSSENVVLVNGGSIVDALAATPFTSAINSPILLTQKEALNSKTKVEIQRLG
                     AKKVYLIGGENSISKETEQQLKSLNISTERISGSDRYKTSLLLAQKLNSIKNVSQIAV
                     VNGVKGLADAISVGAAAAENNIPIILANEKSELQGADEFLNSLNIEKSYIIGGTASLS
                     NNLESKLKNPTRLSGSSRDETNSKIIDNFYKKDTLKNVFVVKNGIKSQNDLIDGLAVG
                     PLGAKTGSPVVLVGDKLADSQKEVLKNKTLEKVTQVGGGANQNAFKELVKLKSVK"
     gene            complement(8608..9363)
                     /locus_tag="L3M60_002787"
     CDS             complement(8608..9363)
                     /locus_tag="L3M60_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HBN6227429.1"
                     /translation="MFSIGVDSGSVATKGVLFDGEKIIKKIIIPTGWSPKSTSKQVYE
                     LLSSEIDKKDIKKVVGTGYGRGVMDFADKKVTEITCHTRGVYFLNNNVRTILDVGGQD
                     SKVINLDRDGNVFNFIMNDKCAAGTGRFLEITSNLLGSDIESIDTLAQGHEPVNISSM
                     CTVFAESEIVSLLAQNISAGEVAAGILKSIANKSTSMLARGEIIDEVAFTGGLAKSKE
                     LVKMIEEILDKKIFIAEDTQIIGALGAAVIGFR"
     gene            complement(9388..10539)
                     /locus_tag="L3M60_002788"
     CDS             complement(9388..10539)
                     /locus_tag="L3M60_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBN6227430.1"
                     /translation="MTNLPKQFDSFNEARQKGFINAKELKESGKKMVGVFCTFTPVEI
                     PMAAGATVVGVCGVSEEPIPDAERVLPRNLCPLIKSSYGHAITDTCPYFYFSDLLIGE
                     TTCDGKKKMYEELAKVKPTYVMHLPNTSKGEFAYKLWKDEMIRLKEEVEKSLGVTITE
                     EDIRAAIKDKNEERELLKEFYALGKLQPSALTGLELHNVLYQAGFKFDRAELKHSLRK
                     VIDDMKERYEKGECPVKKDKPRILITGSPIGGISEKIVKTLEDAGASVVAYELCGAIR
                     SNDLLVDEEIEDVYDALTQKYINIGCSCMMNNDNRIELLDRIIDEYNVDAVIDVVLQA
                     CHTFNIESYRIREFVTKEKNKPFMSLETDYSKSDTEQLRTRFEAFVEML"
     gene            complement(10748..>10919)
                     /locus_tag="L3M60_002789"
     CDS             complement(10748..>10919)
                     /locus_tag="L3M60_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HBN6227431.1"
                     /translation="RTFQQEYRDYLDLAKFLQLMPGIGAFVGFYVNGKLIDKLCETAV
                     SAYRLRIRELKL"
BASE COUNT         3626 a         1877 c         1091 g         4325 t
ORIGIN      
        1 tttaaattta atacttgtca ataaatttta attttaataa attttaatca tcatagtgaa
       61 aaaatttttt tatataaaac taatcaaaaa aagatatctg tacatctcct gagtttttta
      121 acttttcttt cattatttta gagtaactgc ttgatagaga atatttatat ctaagttcat
      181 taaccatttt atacaattga tttttatacc ccctattaag tgaacctgtg ctatatagta
      241 ttgataattt atcaaataat tcaggaaact cttttttaac aaagtcaaaa aaaatacctc
      301 tagttatacc ccttaagtat aaagtcccag gtaaaacata atgtacatta cattcttttg
      361 catgtctaaa tagcaaattt atattatcaa aatcatctgt aatatatggt ataattggca
      421 taatatgtag accaactgat gcgttagttt ttctaaattc ttttaacatt ttaaatcgtc
      481 taattgaatc cacaccattt ggctcaataa gcctttgtgt atcttcattt actgtggtaa
      541 ttgtagatgc tacattgata tatgtaatcc tagatagctt atcaattaaa tcgtaatctc
      601 ttaagattaa atctgatttt gtagaaataa tagctggtgt cttgtacttt atcagcaaat
      661 ttaatatttc tggcataatt ttatagtcag cttctattgg ttgataacta tctgtaactc
      721 ctcctatatt gattacttct ttcttccatt tactgctttt aagttgtctc tcaagctttt
      781 ccactatatt tgtttttaca taaatttctt caaaataatt attggagttg atatacttat
      841 gagagtaaat agcatagcag tatttacacc catgctcaca tccacgataa atattcaaat
      901 cccatttata tggcatactc ctttttagtt tattacaagc tatttcacaa tttattggaa
      961 tatattttat tttattatac aaataatcac ctctttatat acaataatat tataatgtta
     1021 atctatagta attaaaacat gatatttcca taccacaatt cataatcttt actatattta
     1081 caaagtaata tattccattc atttatgggt attcctccac atgttgcttt ttcttctaaa
     1141 ttatataagc attgctttaa atacatttta ttgtgccaac catcatataa attttctaaa
     1201 tctaaactat gttcattaga tatatcattt gcaaaatttc tatttttcca ttttaatata
     1261 cctgtatatt taagttcatt aggtttataa tttcttttta acatttcatc atgtacaatt
     1321 tttgtataaa atttaaattc atctatacta taattcaaca ctttattcac taatatatgg
     1381 tttggagtac cttttttatc tatagaacct tttatagcaa gtagctctct ccattgactt
     1441 actaattgat ttttaggtaa tacatcaatt aaatctttat gccataatct cataatttaa
     1501 ctccttacta taaaatattt tataaattca ataaccaatc tatcttatct atcttacaat
     1561 tttacatgtt caataatttt atctgtaaaa actataccat ttaaatggtc tatctcatga
     1621 caaagacatt ttgccataaa acctgaacct ttaatttcta ttttctcgcc gtattcgttt
     1681 agagcttgca ctgtaacttt ttttggtctt tttaatttac cccatacttc aggaaaactt
     1741 aaacatcctt caacaacaat ttgttcccct tcttgtttaa taatttcagg attaacaagc
     1801 ttaataagtc cttctcctaa atctataaca actagtcgct ttaatactcc tacttggcat
     1861 gcagctaatc ctcctccatt tggagtatta tacatggtct ctgccatgtc atttaatatt
     1921 tctcttatct tattatctac gttttctaca tacttactct tttttcttaa aatttcatca
     1981 tcaaatgttc ttatctttct tatagccata atattctccc ctacttatct gtgctcttga
     2041 ttttctttca ctttcttcaa ataaaaatac actttcaatt ggttcattaa aaattcttga
     2101 aatatcatat gccaaccata ttgaaggttc atttttacca gtttctatag catttatagc
     2161 ctgtctagat gttccaacta atcttcctag ttgttcttgt gtcaatccta atttttctct
     2221 taatatcttt attttatttt tcatatttgc tccattcatg taatgttaac cttacatttt
     2281 aaatgtaaca tttttcaatt ttactgtcaa gtcaacctta catttatata ttaaattgtc
     2341 ttatttaact atttttttac taaacttttt aaatagtagt aaaatccata ttaatgatat
     2401 aataatggaa ataaacatat agatataatt atcttcctta aatgcctttg atagccaaaa
     2461 agttggtaag aagaacagaa tatattgcac tttatttaaa ataaaaaatg gtgctaaaat
     2521 acccatcata aaaacacctg agaatttcgt aattaccatt ccttctagtt tatttgatga
     2581 taaagaaatc actaatattg atataataac gctttgtaat aaaactaata aagaaattga
     2641 taagtttaac aaaaaagaaa ttctagttag agaaaaaaag ataagtagaa tcatagtaat
     2701 aataaatgct aagatacctg gtattccaaa tcgtgaaaca agataaccag ttttgccaag
     2761 cggagttact attaaatacc ttgaaatact atcatcaatt tctcccaata cgaccattgt
     2821 agaagcaaaa aagaacataa aaggtgtaat aaaagctaaa agtaaatcaa acatcaataa
     2881 atacggttct aaatttaatt gataataaaa cttgttaagt gatatattct gtattaaggg
     2941 tattccaaat tttataaata ctccacataa tattggagag acacatacta taaaaagcat
     3001 agcgtctctt ttaatttgtt caaactcttg tttaaacgca tttataatct ttatcatagt
     3061 tttactccac ctaccttttt ccaactacta caaataaaat aataagttat aaaataaatt
     3121 attattatca ttgatattaa aattaaaata ttaatcacta taaaattttt atctccacta
     3181 ataagagaaa tacatatatt aaaaggataa aaatttgcat atgattttgt atcaacaagt
     3241 acattgagta ttggtggaat aaagcatata atctcaatag gaatacttaa aacaataaac
     3301 tgattcaaac tagatgcttt acttgcaagt atcaatccaa gcaaagaaaa tataatagaa
     3361 gaaaaaaaag tccctatagt tgatattata atattatcta aatttaatgt aatagcaata
     3421 agcatagcta caatactaga tattactcct aatgaaatta cctttgataa aatatactca
     3481 gatactttta ctggagatac agctaaggaa tttaaaaccc tttgactttt ctcaagaagt
     3541 acaatagctc ccataaaaaa caatcccatt gctgctgggt cagaataaat cataattata
     3601 gctatttttt ctctcataaa agttggaaat acattgataa tacaaatata tataatactt
     3661 aaaaaaagat ataaaaaata aaaaccatat ttaaattgaa actttatatc tcctaaaaca
     3721 agatttttta gcctcattgt aaattcctcc ctgttatctc aataaaaata tcatttaatg
     3781 ttggttcact actatgtatt gataaaagtt tatttttctc aataagcata ttcaaatttt
     3841 tatcattagc agtattattc agaaaacatt cagaagtttt ttctccatta tcaaagtaag
     3901 tatatttaac tttaatagct ccttttgaca taataagatt atgtggtgta tctaaagcag
     3961 atattttacc atttactatg aatgctactc tgtcacaaag ctctgttgca tctaacatgt
     4021 tatgagttgt taaaataata gttttacctt tagatttttc tgataaaatt atatctttca
     4081 taaccttgct atttgatggg tcaagaccac ttgttggttc atctaaaaat aaaatatctg
     4141 gattatgtaa taaagctttt ataaagttta atctagattt catacctttt gaatactcag
     4201 ataccctttt atttgcttta ttttctagcc ctactgattt taataactta tcaatagata
     4261 gtaatttttt agaatataga gacccaaaat attttaaatt ttctattgca gtcaactttt
     4321 catataaact aggaaattca aaatcaactc ctatattttc ataaaattta tttgagtgcc
     4381 ttttactctc tactccattt acaatgacac ttcctttata attagttatc attccaatta
     4441 gaatcttttg taatgtagat tttcctgcac ctgatggacc taaaaaacct agtatttcac
     4501 cttttcctac ttcaaaatta atattttgta aaaaattttt atctgtataa ctaaatgata
     4561 aatcatcaac tttaatcatt aaatatcacc ctttcaacct tttgaccatt ttagctatat
     4621 agtcaataaa aaataatttt tttgtttgtt aataattttt aataaaagtt attagcaaac
     4681 aaaatttaaa ttctattcca taagttgtat aactaatcca tttatcatta atcttaaagc
     4741 atcttcaaaa caattactcc caacctcacg cttatataac atggtaagaa agactgctct
     4801 aaatgctcca ctaaatcttt ctatatcttt attttttgcg tttggtatgc aagatataat
     4861 ctctccaatt tcaaaatcat catgtctaaa atgttcttca attatagcat ctggcaactt
     4921 tctcactaat atctcaaatt ctccatttac catgagattt aagattgaag tactatcaac
     4981 catcctatag aatttcataa atatatctgt caattgttca catgtaatgc ttttatctag
     5041 tctatttatc tcatataaga tttctttctg tatagagtca tgtatatctc tcaatacttc
     5101 aaaaaaaagc aactcttttg attgaaagaa tagataaaaa gttccttttg gtatttttac
     5161 tcgttttaca agctcatcaa cagtagtttt tctaatacca tatagctcca tacacaatct
     5221 tgcttcttct ttaagtcttt ttattatata ctctctttct ttgtcagaat atgatttagg
     5281 catcttccgt cctccagttt gactaaaata ttttatatag tcaatatatt actttttcta
     5341 tttaatgtca atagttttgc tagtatattt aaaaatagac ttaaaaactt tcgaaaattc
     5401 tgaattttat atagcatttt ttattattta atgttataat taagatataa ataatatatt
     5461 attctagttt tcatatctat ttgcttattt tttaacttag tttctaatag aagtaatcat
     5521 atttatcttt ttaaaagttt cagctttaat aaaaaataaa ggagttgatt ttatgactac
     5581 tactacaaag aaccgttttt atgattatgt tcaagaaaat ttaaataaat acgttgaagt
     5641 acattcttac ttcagcagtt gtcctctata tggtgaaatt atagaagcag acagcttatc
     5701 aattacactt tgctcaagat atatagacga atctaatcca actttaaacg aaacttacac
     5761 attatattta ccattaagtt caataatatc tattagaaca ctataaaaag ttaattaaat
     5821 atgggttatt tatatataaa atgtaagatt ttctatataa agtaaccctt ttttatgttt
     5881 ttaaacttat actacataaa tttaaagctg aaaacaaaac tttgcaacca ttgattatct
     5941 tatttatcaa taatttaaag taattatttt cagctcttat cagtataatt tactaataca
     6001 tattaatata taccaatacc taagtatcct atttaacact ctttaatttt actaactctt
     6061 taaatgcatt ttgatttgct cctccaccaa cttgagttac tttttctaaa gtcttattct
     6121 taagaacttc tttttggcta tcagctaatt tatcaccaac taaaactact ggtgacccag
     6181 tttttgctcc taaaggaccc actgccaatc catctattaa atcattttga ctcttgattc
     6241 catttttaac tacaaataca ttttttaaag tatctttttt ataaaaatta tctattatct
     6301 ttgaattagt ttcatctcta ctactaccag ataatctagt tggatttttt aacttacttt
     6361 ctagattgtt tgatagggat gctgttcctc caataatata agatttctct atatttaatg
     6421 aatttaaaaa ttcatcagct ccttgcaatt cactcttttc atttgcaagt atgattggta
     6481 tgttattttc tgctgctgct gcacctacac taattgcatc agctaatccc ttcactccat
     6541 ttacaactgc tatttgagat acatttttta tactatttaa cttttgagct aacaataaac
     6601 ttgtcttgta tctatcactt cctgatattc tctctgttga tatatttaag ctttttaatt
     6661 gttgttcggt ttctttactt atagaatttt ctccacctat taggtatact ttcttagcgc
     6721 ctaatctctg tatctcgact ttagtttttg aatttaaagc ttctttttgt gtcaataata
     6781 ttggtgaatt tatagcagat gtaaaaggag ttgctgccaa tgcatctact attgaacctc
     6841 catttactaa aactacattt tctgaagaat tgaaaccatt ttgactaact ttgatagcag
     6901 tttcatatct atcactacca attaaagact catttggtac actctcacta gaagactttt
     6961 ctgtaacagc ttttatagta aaattacatg gatagaattt actatctctg tttatatcaa
     7021 agaactttcc agtattatct ataatataac tctcacctaa attagctttt gctttaaatg
     7081 tatcatatcc tggcacgttt gactcagagc ctatagttga ttctccattt ttcccctcta
     7141 tactgactac aattgcacct tttccttctg gtatatcaaa agaatcaatt ggaactgtta
     7201 tatatcctga atattgtgta gtaccttctt ttaatttttt catattattt tcttgaggta
     7261 taccatttac tactggagca taatatactt catatttagc tcctatactt tcagttaaaa
     7321 acattacaga ttttagagtc tcatcacctc tattaaaatc gaatacattt gctgctgtta
     7381 ttttatttga gattaaagga acaatgcttg catagtcatg ttgatacatt ttctcatcat
     7441 cacttggttt ttgaccacct ttgattgaaa aattatctat atcagacata agtgtcttgt
     7501 cttcataaga tatccagaaa tatccacctt cagagttata atctccccag ctatttttta
     7561 ttagccaagc accatttttt gatggtctaa tatttgaatt aaaattactt ttagaatagt
     7621 tatcatccca gcctacaatt gatatagcat gatttgtatt aaaacttttt gttggacaat
     7681 aatatgaatt ttggtcatca ctttgatatt taatatcttc ataataggca gatgttactg
     7741 ccccatactg taatatagca ttttttgtag aattaatgtc attacttaca cacacagcat
     7801 ctgttacatt aaaaacagta ggtgctgtat tcatattagt tggttttgtt gctccttgtg
     7861 ctttagatat tttaccatta tacgggatat ctttttctaa tttaggacct gagcaagatg
     7921 ttaaatatcc catagcttca taatttaaag caccatccat atcatttaca gaccatccat
     7981 attcaccatc tgaagcccac catcttaaat gttcttctga taaatcgtaa tctccataat
     8041 tatttaactt caagaagctt tctaaagttg ccattgcagc aaaatcccaa catattccta
     8101 aattctcttg attttttact gatgtcataa gacctaattt tctagggtca taagatgttg
     8161 gagctgttct tcctttactt ttaatttgag taccttccaa ttgatatgga tttggtataa
     8221 tcccattata tttttctgta cgtccatttt taacatcatt tagatattgt ttataagtct
     8281 cactgtattc tgcttttttt atctgattca atgtattgct agtgtcagct gatgcaggaa
     8341 ttgttgacat tactaaaata gatattaaaa acagtgcaac aaaccttgct actttttttg
     8401 aagtaaatct tttcacgctt tctccccctt ttttgagcta atttttaaaa ttctaatgtt
     8461 atatatgact aaatatcaag tatttacaaa tatacaaaca tttaaatcaa ttataccaaa
     8521 gttttccttt ataaataaat aaaataaaat aaaaaactga atttatttag gataaactca
     8581 gtttctcatt ttattttatt tatattttta tctaaaccct attacagcag ctcctaaagc
     8641 tcctattatt tgagtatctt ctgcaataaa aattttttta tccagtattt cttctatcat
     8701 tttcactaat tccttacttt ttgcaagccc tcctgtaaat gcaacttcat ctattatctc
     8761 acctcttgct agcattgaag tggatttatt tgcaatagat ttaagtatcc ctgctgctac
     8821 ctctccagca gaaatatttt gagctagaag acttacaatt tcagactcag caaaaactgt
     8881 acacatacta gatatattaa ctggctcatg tccttgagcc aatgtatcaa tactttctat
     8941 atcacttcct aaaaggttag aagtaatctc taaaaatcgc ccagtacctg cagcacactt
     9001 atcattcatt ataaaattaa atacatttcc atctctatct aaatttataa ctttactatc
     9061 ttgaccacct acatctaaaa tagttcttac gttattgttt aaaaagtaaa ctcctctagt
     9121 atgacaagtt atttctgtta ctttcttatc agcaaaatcc attactcctc ttccataacc
     9181 tgtacctact acttttttta tatctttttt atcaatttca ctacttaaca actcataaac
     9241 ttgtttagat gtacttttag ggctccatcc agttggtata attattttct taataatttt
     9301 ttcaccatca aacaatacac cttttgttgc cactgaccct gagtccactc ctatactaaa
     9361 cataattcct cctattttta tgtatttcta caacatctct acaaatgctt caaatctagt
     9421 tctcaattgt tcagtatctg attttgaata atcagtttca agagacataa atggcttgtt
     9481 tttttctttt gtaacaaact ctcttattct ataactttct atattaaatg tatgacatgc
     9541 ttgtaaaact acatctatta cagcatctac attatattca tctattattc tatctaataa
     9601 ctcaatacga ttatcattat tcatcataca agaacaacct atatttatat atttttgtgt
     9661 taaagcatca tacacatctt ctatttcttc atcaaccaat aaatcatttg atctaatagc
     9721 tccacaaagt tcatatgcaa cgactgatgc ccctgcatct tctaaagttt taactatttt
     9781 ttcacttatt ccaccaatag gagaacctgt tatcaatatt cttggtttat cttttttaac
     9841 aggacattct cctttttcat atctttcttt catatcatct ataacttttc ttaaagaatg
     9901 tttaagttct gcacggtcaa atttaaaacc tgcttgatat aaaacattat gtaactcaag
     9961 acctgtaagt gcagatggtt gtaatttacc aagtgcataa aattctttta aaagttctct
    10021 ttcttcattt ttatctttta tagcagctct tatatcttct tcagttatag ttacacctaa
    10081 agatttctca acctcttctt ttaatcttat catttcatct ttccaaagtt tataggcaaa
    10141 ttctcctttt gaggtatttg gcaaatgcat tacatatgtt ggcttaactt ttgcaagttc
    10201 ttcatacatt tttttcttac catcacaagt ggtttctcct atcaacaaat cagaaaaata
    10261 gaaatatgga caggtatctg ttattgcatg cccatagctt gatttaatta aaggacaaag
    10321 atttcttgga agcactcttt ctgcatcagg tattggctct tcactaacac cgcatacacc
    10381 tactacagtt gctccagctg ccattggaat ttcaactgga gtaaatgtac agaaaactcc
    10441 aaccatcttt ttcccacttt ccttaagttc ctttgcattt ataaatcctt tttgtctagc
    10501 ttcattaaag ctatcaaatt gttttggcaa atttgtcatg aaaatattcc tcccaatcac
    10561 ttctcttcat aatccaattt gtatcttaaa cattaaaaac taaatttttc cattaaaata
    10621 caaacctcta cattagattc tatcatgctt taccttttat ccataattgt ttttatcaat
    10681 gcaaaattaa atgtatatag atatattgta taactaattt tttatgttat aacttctctt
    10741 ataactttta taacttaagc tctcgaatcc tcaatctata agcagacaca gcagtttcac
    10801 ataatttatc aattaatttt ccattcacat aaaatcctac aaatgctcct attccaggca
    10861 ttagttgtaa aaacttagct aaatctaaat aatctctata ctcttgttga aatgttctc
//
LOCUS       DAFNNB010000129        10888 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0131, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000129 DAFNNB010000000
VERSION     DAFNNB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0131"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            153..3869
                     /locus_tag="L3M60_002790"
     CDS             153..3869
                     /locus_tag="L3M60_002790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBN6227432.1"
                     /translation="MPHPVTIGKRTRMSFSKIKEIADVPNLIEIQVDSYEWFLKEGLK
                     EVFDDISPIEDYTGNLILEFVDYSLDDKPKYDIEECKERDATYCAPLKVKVRLINKET
                     GEIKEQEVFMGDFPLMTERGTFVINGAERVIVSQLVRSPGVYYAEERDKTGKRLISST
                     VIPNRGAWLEYETDSNDVISVRVDRTRKQPVTVLLRALGIGTDAEIIDLLGEDERLSA
                     TLEKDNTKTVEEGLVEIYKKLRPGEPPTVESASSLLNALFFDPKRYDLAKVGRYKFNK
                     KLALCYRIMNKISAEDIINPETGEVFVKAGEKISYDLAKAIQNAGINVVNLLMDDDKK
                     VRVIGNNFVDIKSHIDFDIDDLNIKEKVHYPTLKEILDGYSDEEEIKEAIKSRIKELI
                     PKHILLDDIIASISYEFNIFYNIGNIDDIDHLGNRRIRSVGELLQNQVRIGLSRMERV
                     IKERMTVQDMEAITPQALVNIRPVSAAIKEFFGSSQLSQFMDQTNPLSELTHKRRLSA
                     LGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLINSLGTYAKINEFGFIESP
                     YRKFDKETSTVTDEIHYLTADEEDLFVRAQANEPLTEDGKFVNHRVVCRTVNGAVEMV
                     PESRVDYMDISPKQVVSVATAMIPFLENDDANRALMGANMQRQAVPLVRREAPIIGTG
                     IEYRAAKDSGAVVVARNSGIAERVTADEIIIKREDGNRDRYNLLKFKRSNSGTCINQT
                     PIINKGDQIMKGDVIADGPATDLGEVALGRNCLIAFMTWEGYNYEDAILINERLVKED
                     RLSTIHIEEYECEARDTKLGPEEITRDIPNVGDSAIKNLDDRGIIRIGAEVDSGDILV
                     GKVTPKGETELTAEERLLRAIFGEKAREVRDTSLKVPHGESGIIVDVKVFTRENGDDL
                     SPGVNELVRCYIAKKRKIKVGDKMAGRHGNKGVISRVLPEEDMPFMENGTPLDIILNP
                     QGIPSRMNIGQVLEVHLGLAAKTLGWYVATSVFDGANEYDIMDALEEAGYPRDGKLTL
                     YDGRTGESFDNRITVGYMYYLKLHHLVDEKLHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAHILQEILTVKSDDVVGRVRTYEAIVKGENIPEPGIPESFKVLIK
                     ELQSLCLDVKVLTDEDQEIEVRESVDEDDTIGEFELDVVNHMGEVEESNIIEEIEDDF
                     AENAEDEDIENLEEFTEDDLFEEEIDFDSDDFDM"
     gene            3911..7396
                     /gene="rpoC"
                     /locus_tag="L3M60_002791"
     CDS             3911..7396
                     /gene="rpoC"
                     /locus_tag="L3M60_002791"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBN6227433.1"
                     /translation="MFELNNFESIKIALASPEKIRQWSRGEVKKPETINYRTLKPEKD
                     GLFCERIFGPQKDWECHCGKYRRVRYKGVVCDRCGVEVTKSKVRRERMGHIELAAPMS
                     HIWYFKGIPSRMGLLLDMSPRSLEKILYFASYVVVDPGETGLNEKQLLTEKEYRTALE
                     KYGYTFTVGMGAEAVKTLLQNIDLEQQSKDLRAELKDSTGQKKVRTIRRLEVVEAFKK
                     SGNKPEWMILDAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLELGA
                     PDIIVRNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPELKFYQCGLPKKMALELFKPFVMDKLVKEGYAHNIKSAKSIVEK
                     VKPEVWDVLEDVIKSHPVLLNRAPTLHRLGIQAFEPILVEGKAIKLHPLVCTAYNADF
                     DGDQMAVHVPLSVEAQAEARFLMLSVNNILAPKDGSPITTPSQDMVLGCYYLTIEAQD
                     GAKGTGMVFKDFNELLLAYYNKSVHLHALVKLKVTLEDGRSSLVESTVGRFIFNEKIP
                     QDLGFVDRKENPFALEVDFLADKKSLGKIIDKCFRKHGNTETAELLDYIKSLGFKYST
                     LGGITVAVDDMSVPEEKKVFIAEAEAKVDKYEKAYRRGLISDEERYEKVIETWTETTD
                     KVTDALMGGLDRLNNIYIMAHSGARGSKNQIRQLAGMRGLMANASGKTVEIPVKSNFR
                     EGLSVLEYFTSSHGARKGLADTAIRTAESGYLTRRLVDVSQDVIVREVDCGTEDTTEI
                     YAIKEGNEVIEEIYDRIVGRYTIDPILNPETGEVIVEADSMIQEDEAETIVALGIEKI
                     RIRTVLNCKTNHGVCSKCYGRNLATGKEVNIGEAVGIIAAQSIGEPGTQLTMRTFHTG
                     GVAGADITQGLPRVEELFEARKPKGLAVITEVSGRVEIDETGKRKEVNVIPEEGETQT
                     YVIPYGSRLKVKQGQMLEAGDPLTQGFINPHDIVRVNGVKGVQEYIVKEVQRVYRLQG
                     VDVNDKHIEVIVRQMLSKVKVEDPGDTDLLPGGYEDVLTFNECNKDAIDKGLRPAVAK
                     RVLLGITKASLATDSFLSAASFQETTRVLTEAAIKGKEDHLIGLKENVILGKLIPAGT
                     GMKKYRNIAVEKIED"
     gene            7686..8108
                     /gene="rpsL"
                     /locus_tag="L3M60_002792"
     CDS             7686..8108
                     /gene="rpsL"
                     /locus_tag="L3M60_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBN6227434.1"
                     /translation="MPTINQLVRKSRKALEKKSTAPALQKGYNSLNKKVTDASAPQKR
                     GVCTSVKTVTPKKPNSALRKVARVRLTNGIEVSAYIPGEGHNLQEHSVVLIRGGRVKD
                     LPGVRYHILRGTLDTAGVDKRRQSRSKYGAKRPKEAKK"
     gene            8231..8701
                     /gene="rpsG"
                     /locus_tag="L3M60_002793"
     CDS             8231..8701
                     /gene="rpsG"
                     /locus_tag="L3M60_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBN6227435.1"
                     /translation="MPRKGNIPKREVLPDPMYGSKVVTKLINNLMVDGKKGKSQRIVY
                     DAFAIVAEKTGEEALEVFNKAMDNIMPVLEVKARRVGGANYQVPIEVRPERRQTLGLR
                     WLVKYTRARGEKGMVEKLAKEIMDAANNTGASVKKKEDTHKMAEANKAFAHYRW"
     gene            8750..10816
                     /gene="fusA"
                     /locus_tag="L3M60_002794"
     CDS             8750..10816
                     /gene="fusA"
                     /locus_tag="L3M60_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBN6227436.1"
                     /translation="MARKFPLEKTRNIGIMAHIDAGKTTTTERILFYTGQTHKIGETH
                     EGASQMDWMEQEKERGITITSAATTASWKDHRINIIDTPGHVDFTVEVERSLRVLDGS
                     VAVFCAKGGVEPQSENVWRQAETYGVPRIAFINKMDILGADFYNVVSMMKSRLNSNAV
                     PMQLPIGKEDSFIGIIDLLKMDAVIYKDDLGVEMEETDIPEDMKELAAEWREKLVESV
                     AETDEELMMKYLEGEELTIDELKVAIRKATIACEMNPVFCGTAYRNKGVQLVIDAVLD
                     YLPAPTDIPAIKGILADGEEAERHSSDEEPFSALAFKIMTDPFVGKLAFFRVYSGTLE
                     SGSYVLNATKNKRERIGRILQMHANTREEITKVYAGDIAAAVGLKDTTTGDTLCDPAN
                     PIILESMEFPEPVISVAIEPSSKAAQEKMGIALQKLAEEDPTFTVKTDQETGQTIISG
                     MGELHLEIIVDRLLREFKVEAKVGAPQVAYRETITQPVDVEYKYSKQSGGRGQYGHVK
                     IRVAPQEPGEGYKFTNKTVGGSVPKEYVGPVDMGIQGAMQSGIVAGYPVVDVAVELYD
                     GSYHEVDSSEMAFKMAGSMAFKDAMKKGNAVLLEPYFKVEVVTPEDYMGDVMGDLNSR
                     RGLIQGMEARSGAQVINAFVPLSEMFGYSTDLRSSTQGRATYTMIFDHYEQVPASVAK
                     KIAEGK"
BASE COUNT         4212 a         1416 c         2280 g         2980 t
ORIGIN      
        1 gcttaaaata atttacaaaa aatcaaaaat aatgcatagt ttacatataa gataaaaaat
       61 atattgaaaa atacttgaca ttatgctaaa attattagat gcgttagaat gaactattgt
      121 tttattttta attattgaga ggtgaaaagt gaatgccaca tcctgtcacg ataggtaaaa
      181 gaactagaat gagcttttct aaaattaagg aaatagctga tgtgccaaat cttatagaaa
      241 tccaagtaga ttcctatgag tggtttttaa aagaaggttt aaaagaagtg tttgatgata
      301 tttcaccaat agaagattat actggtaatc ttatattgga atttgtcgac tattctctag
      361 atgataaacc aaagtacgat atagaagagt gtaaagagag agatgctaca tattgtgcac
      421 ctctaaaggt taaggtaaga cttataaata aagaaacagg tgagataaaa gaacaagaag
      481 tatttatggg tgacttccct ttaatgacag aaagaggaac tttcgttata aatggagcag
      541 aaagagttat agtaagtcaa ttagttagat ctcctggtgt ttactatgcg gaagaaagag
      601 ataaaacagg taagagattg atttcatcta ctgtaattcc taatagagga gcatggttag
      661 aatatgaaac agattctaat gatgtaatat ctgtaagagt tgatagaaca agaaaacaac
      721 cagttacagt tttacttaga gctttaggaa taggaacgga tgcagagata attgaccttt
      781 taggagaaga tgagagatta tctgctacat tagaaaaaga taatactaag acagtagaag
      841 aaggtcttgt agagatatac aaaaaattaa gaccaggtga acctcctact gtagaaagtg
      901 catcatcttt actaaatgct ttattctttg acccaaaaag atatgaccta gcaaaagttg
      961 gtagatataa attcaataag aaacttgctt tatgttatag aataatgaat aaaatttcag
     1021 cagaagacat aataaatcca gaaactggtg aagtatttgt aaaagctgga gaaaaaataa
     1081 gctatgattt ggctaaagcc atacaaaatg caggtataaa tgtagtaaat ctactaatgg
     1141 atgatgataa gaaagttaga gttataggaa ataattttgt ggatattaag tctcacattg
     1201 attttgacat agatgattta aacataaaag aaaaagtaca ttatccaact ttaaaagaaa
     1261 tattagatgg atatagtgat gaagaagaaa taaaagaggc tataaaatca agaattaaag
     1321 aacttatacc aaaacatata ttattagacg atataatagc ttcaataagt tatgagttca
     1381 atatattcta caatatagga aatattgatg atatagatca cctaggaaac agaagaataa
     1441 gatctgtagg tgaattgtta caaaatcaag ttagaattgg tctttcaaga atggaaagag
     1501 ttataaaaga aagaatgaca gttcaagata tggaagctat aacgcctcaa gcgttagtta
     1561 acataagacc agtttcagct gcaataaaag aattttttgg tagttctcag ttatctcagt
     1621 tcatggatca aacaaatcct ctatctgagt taacacataa gagaagacta tcagcccttg
     1681 gacctggggg tctttcaaga gaaagagctg gatttgaagt gcgtgacgtt caccattcac
     1741 actatggtag aatgtgtcca atagagactc cagaaggacc aaatatcggt cttataaact
     1801 ctctaggaac ttatgcaaag ataaatgaat ttggatttat agaatcacca tatagaaaat
     1861 ttgataaaga aacatcaaca gttactgatg aaatacatta tttaactgct gacgaagaag
     1921 atttattcgt aagagctcag gcaaatgagc cattaacaga agatggtaaa ttcgtaaacc
     1981 atagagttgt ttgtagaact gtaaatggtg ctgttgaaat ggttcctgaa agtagagtag
     2041 attacatgga tatatctcct aagcaagttg tatcagttgc cactgctatg atacctttct
     2101 tagaaaatga tgatgctaac cgtgccctta tgggagcaaa catgcaacgt caggcagtac
     2161 ctctagttag aagagaagca ccaattatag gaactggtat agaatacaga gcagcaaaag
     2221 attctggagc agttgttgtt gctagaaact ctggtatagc agaaagagta acagcagatg
     2281 aaataattat aaaaagagaa gatggaaata gagatagata taatctactt aagtttaaac
     2341 gttcaaactc aggtacttgt ataaatcaaa cacctataat aaataagggt gaccagataa
     2401 tgaagggtga tgttatagca gatggtccag caacagactt aggagaagtt gcacttggaa
     2461 gaaactgcct tatagcattc atgacttggg aaggttataa ctacgaggat gctatattaa
     2521 taaatgaaag attagttaaa gaagacagat tatcaacaat tcatatagaa gaatatgaat
     2581 gtgaagctag agatacaaaa ctaggaccag aggaaataac tagagatata cctaatgttg
     2641 gagatagtgc aattaagaac ttagatgaca gaggtataat aagaataggt gcagaagtag
     2701 attcaggtga tatactagtt ggtaaagtaa ctccaaaagg agagactgaa cttactgctg
     2761 aggaaagatt acttcgtgca atatttggag aaaaggctag agaagttaga gatacttcac
     2821 ttaaagtacc tcatggtgaa tctggtataa tagtcgatgt aaaagtgttt actagagaaa
     2881 atggagatga tttatctcca ggagtaaatg aattagtaag atgttatata gctaaaaaga
     2941 gaaaaataaa agttggagat aaaatggctg gtcgtcatgg taataaaggg gttatctcaa
     3001 gagtgttacc tgaagaagat atgccattca tggaaaatgg aacaccacta gatataatac
     3061 ttaacccaca aggtatacca tcacgtatga acattggtca ggttctagaa gtccatttag
     3121 gacttgctgc taaaacatta ggatggtatg tagcaacatc agtattcgat ggtgctaatg
     3181 agtatgatat aatggatgct cttgaagaag ctgggtatcc tagagatggt aaattaactt
     3241 tatatgatgg tagaacaggt gagtcttttg acaatagaat aactgttgga tatatgtatt
     3301 acttgaaact acatcacttg gtagatgaaa aactacatgc aagaagtact ggaccatact
     3361 cattagttac tcaacagcca ctaggcggta aagcacaatt tggtggacaa agatttggag
     3421 agatggaggt ttgggctcta gaagcatatg gggctgctca catacttcaa gaaatactta
     3481 ctgttaagtc tgatgacgtt gtaggtcgtg ttagaacata tgaagctata gttaaaggtg
     3541 aaaatatacc tgaaccagga atcccagaat catttaaggt tcttataaaa gaacttcaaa
     3601 gtttatgctt agatgtaaaa gtattgacag atgaagacca agaaatagaa gtaagagaat
     3661 ctgtggatga agatgataca ataggtgaat ttgagctaga tgttgtaaat catatgggag
     3721 aagttgaaga aagtaatata atagaagaaa ttgaagatga ttttgcagaa aacgcagaag
     3781 atgaggatat agagaattta gaagaattca ctgaagatga tttatttgaa gaagaaatag
     3841 attttgatag tgatgacttt gatatgtaaa tttaattaat gtaaatctca aatagaaggg
     3901 agagaactcc ttgtttgaat taaacaattt cgagtcgata aaaatagcat tggcttctcc
     3961 agaaaaaata agacaatggt ctaggggaga agttaaaaag ccagaaacta taaattaccg
     4021 tactttaaaa ccagaaaaag atggtctttt ctgtgaaaga atatttggac cacaaaaaga
     4081 ctgggagtgc cactgtggta aatatagaag agttagatat aaaggtgtag tttgtgatag
     4141 atgtggagta gaagtaacta aatcaaaagt aagaagagag agaatgggac atatagagct
     4201 agccgctcct atgtctcaca tctggtactt caaaggtata ccaagtagaa tgggactttt
     4261 acttgacatg tcaccaagat cattggaaaa aatattatac tttgcatcat atgtagtagt
     4321 tgatccagga gaaactggat taaacgaaaa acaattgctt acagaaaaag aatacagaac
     4381 tgctcttgaa aagtatggat atacttttac tgtaggaatg ggtgcagaag ctgtaaagac
     4441 gttactacaa aatatagatt tagaacaaca aagtaaagac ctaagagcgg agttaaaaga
     4501 tagtacaggg caaaagaaag ttagaacaat aagaagatta gaagttgtag aagcatttaa
     4561 aaagtctgga aataaaccag aatggatgat tttagatgcg ataccagtaa taccaccaga
     4621 tttaagacca atggtacaac ttgatggtgg aagatttgca acttcagacc taaatgattt
     4681 atatagaaga gttataaata gaaacaatag acttaaaaga ttattagagc ttggagctcc
     4741 agatataatt gtaagaaatg aaaaaagaat gcttcaagaa gctgttgatg cactaataga
     4801 taatggtaga agaggtagac ctgtaacagg acctggaaat agaccactta aatctttatc
     4861 agatatgtta aaaggtaaac aaggtcgttt ccgtcaaaac ttacttggta agcgtgttga
     4921 ctattcagga cgttctgtta tagttgttgg accagagctt aagttctatc aatgtggact
     4981 tccaaagaag atggcattag agctattcaa gccatttgtt atggataaat tagttaaaga
     5041 aggatatgca cataatataa aaagtgccaa atctatagta gaaaaagtta agccagaagt
     5101 ttgggatgtt ttagaagatg ttataaaaag ccatccagtt cttcttaacc gtgcgccgac
     5161 tctgcataga ttaggtatac aagcatttga acctatctta gttgaaggta aagctataaa
     5221 gctacatcct cttgtatgta cagcttacaa cgcagacttt gatggtgacc aaatggcagt
     5281 ccatgtacct ttatcagtag aagcgcaagc agaagcaaga ttcttaatgc tttctgtaaa
     5341 taatatactt gctcctaaag atggttcacc tataactact ccatctcagg atatggtttt
     5401 aggctgttat tatctaacaa tagaagcaca agacggagct aagggaacag gtatggtatt
     5461 taaagacttt aatgaactat tacttgctta ttacaataag tcagttcatc tacatgcatt
     5521 agtaaaactg aaggtaacac ttgaagatgg aagaagttca ttagttgaaa gtactgttgg
     5581 aagatttata tttaatgaaa aaatacctca agacttaggt tttgtagata gaaaagaaaa
     5641 cccatttgct cttgaagttg atttcttagc agataaaaag tctcttggta aaataataga
     5701 taaatgcttt agaaaacatg gtaatacaga gacagctgaa ttgctagatt atataaaatc
     5761 tttaggattt aaatactcta cattaggtgg tataacagtt gcagtagatg atatgagtgt
     5821 tccagaagaa aagaaagtat ttatagctga ggcagaagct aaagttgata aatatgaaaa
     5881 agcatacaga agaggtctaa tatctgatga agaaagatat gaaaaagtta tagagacatg
     5941 gacagaaaca actgataaag ttactgacgc tcttatgggt ggactagata gactaaataa
     6001 tatatatata atggcacatt caggagccag aggttctaaa aaccaaatta gacagctagc
     6061 aggtatgcgt ggtcttatgg cgaatgcatc tggtaaaaca gttgagatac cagttaaatc
     6121 taatttccgt gaaggtttat cagtattaga atactttaca tcttcacatg gtgccagaaa
     6181 aggtcttgca gatacagcta tacgtacagc tgaatctgga tacttaacaa gaagacttgt
     6241 tgatgtaagt caagatgtta ttgtaagaga agtagattgt ggaacagaag atactacaga
     6301 aatttatgct ataaaagaag gaaatgaagt tatagaagaa atatatgata gaattgtagg
     6361 aagatatact atagacccta tattaaatcc ggaaactgga gaagttatag ttgaggctga
     6421 ttcaatgata caagaagatg aagcagaaac tatagtagct ttaggcattg aaaaaattag
     6481 gataagaaca gttcttaact gtaaaactaa tcatggagtt tgttctaagt gttatggtag
     6541 aaacttggct acaggaaaag aagttaatat aggtgaagca gttggtataa tagcagctca
     6601 atctatcggt gaaccgggta ctcagcttac aatgcgtaca ttccatactg gaggagttgc
     6661 aggagctgac ataactcaag gtcttccaag ggttgaagaa ttatttgaag ctagaaaacc
     6721 aaaaggatta gctgtaataa ctgaagtatc tggtagagtt gaaattgatg aaacaggaaa
     6781 gagaaaagaa gtaaatgtaa taccagaaga aggcgagact caaacatatg taattccata
     6841 tggttctaga ttaaaagtta agcaaggtca aatgctagag gctggagacc ctctaacaca
     6901 aggatttata aatcctcatg atatcgtaag agtaaatggt gttaagggag ttcaagaata
     6961 tatagttaaa gaagttcaaa gagtgtatag acttcaaggg gttgatgtta atgataagca
     7021 tatagaagtt atagtaagac aaatgctatc taaagtaaaa gttgaagacc caggagatac
     7081 agatttattg ccaggtggat atgaagatgt attaacattc aatgaatgta ataaagatgc
     7141 tatagataaa ggtctaagac cagcagttgc taaaagagtt ttacttggta taactaaagc
     7201 atctcttgca actgattcat tcttatcagc agcttctttc caagaaacaa caagagtatt
     7261 aacagaggca gcaataaaag gtaaagaaga ccacttaata ggacttaaag aaaatgttat
     7321 attaggtaag ttaataccag caggaacagg aatgaagaaa tatagaaata tagctgttga
     7381 aaaaattgaa gattaatagc atatagagta aagaaatctt gacaaccatt atttgtggtg
     7441 ttaaaatagt aaagtgtgtt gtttaaaagt acactaaggg cttgaacaag ctcttagtga
     7501 cttttagatg gcaataaata agaactaaaa agcatcaagc tagaaaagtg ctaatatcac
     7561 atatttatgt gttatagaca ttaaattgta agtaacgaat gttacaatca gtcagtatga
     7621 ataattcata ttgatgtagt tggcggtcac tgataagatc gaaaatttgg aaggaggtgc
     7681 agatgatgcc aacaattaac caattagttc gtaaaagtag aaaagcgcta gagaagaaat
     7741 ctactgctcc agcattacaa aaaggttaca actctttaaa taagaaagta actgacgcta
     7801 gtgccccaca aaaaagaggg gtttgtactt cagtaaaaac agttactcct aagaagccta
     7861 actcagcttt aagaaaagtt gccagagtta gattaactaa tggtatagaa gtttctgctt
     7921 atataccagg agaaggacac aacttacaag agcatagtgt tgttcttata agaggaggaa
     7981 gagtaaaaga ccttccaggg gttagatacc atatactaag aggtactcta gatacagcag
     8041 gtgttgataa gagaagacaa tcaagatcta aatacggtgc gaagagacct aaagaagcta
     8101 aaaaataaaa caattaatga ttgattatag ttcggtgctt taaatatata tatatttacg
     8161 gcatcacggc attaaatgat ttatgtcgag tacctatgat ttaagttaaa taattaagga
     8221 gggaagcgaa atgccaagaa aaggtaatat tccaaaaaga gaagttttac cagatcctat
     8281 gtatgggagt aaggtagtta ctaaattaat aaataatcta atggtagacg gaaaaaaagg
     8341 aaagtctcaa agaatagttt atgatgcttt cgcaattgtt gctgaaaaga caggagaaga
     8401 agctttagaa gtatttaata aagcaatgga taatataatg cctgttttag aagtaaaagc
     8461 aagaagagtt ggtggagcaa actaccaagt gccaatcgaa gttagacctg aaagaagaca
     8521 aacattaggt ttaagatggc tagtaaaata tactagagct cgtggtgaaa aaggaatggt
     8581 tgaaaagcta gctaaagaga taatggatgc agctaacaac actggagctt cagttaagaa
     8641 aaaagaagat actcataaaa tggcagaagc taataaagca tttgctcatt acagatggta
     8701 ggatatatag atatcttata aattcaagga tatcataagg aggataacca tggctagaaa
     8761 gtttcctttg gaaaaaacta ggaatatagg aatcatggct catatagatg caggaaagac
     8821 tactactaca gaaagaatcc tgttttatac tgggcaaact cataaaatag gagaaactca
     8881 tgaaggagct tctcaaatgg actggatgga gcaagaaaag gaaagaggta taacaataac
     8941 ttctgccgct actactgctt catggaaaga ccatagaata aacataatag atactcctgg
     9001 acacgtggac ttcacagttg aggttgaaag atctctaaga gttcttgatg gttcagttgc
     9061 tgtattctgt gcaaaaggtg gggttgagcc tcaatctgaa aatgtatgga gacaagctga
     9121 aacttatgga gtacctagaa tagcttttat aaacaaaatg gatatattag gtgcagattt
     9181 ctacaatgtt gtaagcatga tgaaatctag attaaattct aatgcagttc ctatgcaatt
     9241 accaataggt aaagaagatt catttatagg tataatagac ttattaaaaa tggatgctgt
     9301 tatatacaaa gacgacttag gggttgaaat ggaagaaaca gacataccag aagatatgaa
     9361 agaattagct gctgagtgga gagaaaaatt agttgagtca gtagctgaaa ctgatgaaga
     9421 attaatgatg aaataccttg aaggtgaaga acttactata gatgaattaa aagttgctat
     9481 aagaaaagct actatagctt gtgaaatgaa cccagttttc tgtggtacag cttatagaaa
     9541 caaaggtgtt cagttagtaa tagatgcagt tttagattac ttaccagcac caactgatat
     9601 accagctata aaaggtatat tagcagatgg agaagaagct gaaagacatt cttctgatga
     9661 agagccattc tctgcattag cattcaaaat aatgactgac ccattcgttg gaaaactagc
     9721 gttcttcaga gtttattctg gaacattaga atctggttct tatgttttaa atgcaactaa
     9781 gaataagaga gaaagaattg gtcgtatatt acaaatgcat gccaatacaa gagaagaaat
     9841 aactaaagtt tatgctggag atatagcagc agcggtagga ttaaaagata ctactactgg
     9901 agacacatta tgtgaccctg ctaatccaat aatacttgaa tctatggaat tccctgagcc
     9961 agttatatct gttgctatag agccaagctc taaagcagca caagagaaaa tgggtatagc
    10021 tcttcaaaaa ctagctgaag aagacccaac tttcacagtt aagactgacc aagaaacagg
    10081 acaaactata atatctggta tgggtgaatt acacttagag ataatagtag atagattatt
    10141 aagagaattt aaagtagaag ctaaagttgg tgcaccacaa gttgcatata gagagactat
    10201 aactcaacca gttgatgttg aatacaagta ctctaagcaa tcaggtggta gaggacaata
    10261 cggtcacgtt aagattagag ttgctcctca agagccaggt gaaggataca agttcacaaa
    10321 caaaactgtt ggtggatctg taccaaaaga atatgttggt ccagttgaca tgggtataca
    10381 aggagctatg caatctggta tagttgctgg atatccagtt gtagacgttg ctgttgagtt
    10441 atatgatggt tcttaccatg aggttgactc ttctgagatg gcgttcaaga tggctggttc
    10501 aatggcattc aaagatgcta tgaagaaagg taatgcagtt cttttagagc cttacttcaa
    10561 agtagaagtt gttactccag aagattacat gggagatgtt atgggtgact taaactcaag
    10621 aagaggatta atccaaggta tggaagctag aagtggtgca caagttataa atgcattcgt
    10681 tccactttca gaaatgtttg gatactcaac agacctaaga tcttcaactc aaggtcgtgc
    10741 aacttacaca atgatattcg atcactatga gcaagttcca gcttcagttg ctaagaaaat
    10801 agctgaagga aaataattta aaaataatta ataatataaa tataaaaaat aatcagctag
    10861 atgagatata tctcatctag cacaagat
//
LOCUS       DAFNNB010000130        10855 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0132, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000130 DAFNNB010000000
VERSION     DAFNNB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0132"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            23..871
                     /locus_tag="L3M60_002795"
     CDS             23..871
                     /locus_tag="L3M60_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBN6227437.1"
                     /translation="MNNQKNLNIPITISSYTLGTEVSFRDRVRIAKEAGFDGIGLRAE
                     NYIDAKNAGVTDEEMIAILNEYDMKVTEVEYITQWGTEEDRTEAQREKEKNVYHMAHL
                     FNVKHINCGLLEMIPEEQIITALGELCDRAGELIIGLEFMPYSGVPDLATAWRVVKGC
                     NRENAMLILDTWHWARAKQTADDLAPIPADKIVSIQVCDVLENPYEKLRDESLHDRLA
                     PGEGFGNTEEFVKIIKEHNILPRVIGAEVISDSLISKGLSTAAETVFNATKKVLDKAW
                     SEVSPK"
     gene            950..2872
                     /locus_tag="L3M60_002796"
     CDS             950..2872
                     /locus_tag="L3M60_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN6227438.1"
                     /translation="MKISSMFTPIRIGSMTVPNRFVVPPMGNNFANTDGTLSETSKAY
                     YIERALGGFGLITIESSVVDKKAKGGPRKPCLYDDSTIDSFKNVIDACHDAGSKVSIQ
                     LQHAGSEGNEKIAGHPLKAASAIPASNGRNTPLAITTEEIYELIESYGDAALRAQKAG
                     ADAVEIHCAHGYLVSSFISQRTNKRVDEFGGCFENRMRLPRLIIENIRKKVGNSLAIL
                     CRINSTDDVLGGIDVHDSSVIAAYLEDCGIDGLHVSRSIHIHDEYMWAPTTLHAGFSA
                     ELVTEIKKAVSIPVITVGRYTEPQFAELMVRQGRCDLVAFGRQSLADPEMPNKAKNGK
                     LDEMIPCIACLQGCVPNMFQGKPISCLANPILGHEAEFKSAEISKEVLVVGGGVGGML
                     ASWICAKRGHNVTLVEKSEVLGGQMRLAAYPPGKGDITNLVRSYISKCNQYGVKICTN
                     TEATVELIKEKSPDAVIIATGATPLVLPIPGINDSGLIHAVDLLDGKKSCGKKVLVVG
                     GGMVGCEVAAFLGEQEHDVTVIELREEVGADVISEHRKFLMNDFENYNIHTVTSAKVA
                     KFYDDGVDYLLADGTEHDLKGFDSVVLAMGSRNYDPLSEVIKELVKETYVVGDAVKAR
                     RALDATKEAFEVALNI"
     gene            2954..3817
                     /gene="aroE"
                     /locus_tag="L3M60_002797"
     CDS             2954..3817
                     /gene="aroE"
                     /locus_tag="L3M60_002797"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBN6227439.1"
                     /translation="MEISGRTGLFALIGTPVGHSKSPVMYNYSFEKLDLDYRYLAFDI
                     TVDKVKEALSAIKTFNIKGANVTMPCKSAVTEYMDELSPAARIIGACNTIVNDNGKLV
                     GHITDGVGYVRNLKENEVEVKGKKVTIMGAGGAATAIQVQCALDGAREISIFNAKDDF
                     YKRAEQTVENIKKEVPKCIVNLYDLEDTNKLYEEIESSDILTNATLIGMKPYDNETNI
                     KDTSVLRKDLVVTDVVYNPRKTKMIEDAESNGCKAIGGLGMLLYQGAESFNLYTGLEM
                     PVKEVNELCFK"
     gene            4019..5236
                     /locus_tag="L3M60_002798"
     CDS             4019..5236
                     /locus_tag="L3M60_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004455016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6227440.1"
                     /translation="MNNKKYYPTAIALYFSYFLLGIGISILGQYKPEFSSMWGAKTLS
                     DGTLDVSIVLAVIAALGLGRLISYPFAGPISDKYGRKVSGLIGNFLHAIFFVGIVFSP
                     NFYIAYIFAIIGGAANSFLDTCVTPSCMEIFASLGTIANMFTKFTIALAQFLLPFIIG
                     FVAANSISFKVIFIVTAILIVVDAILIAVLPFPPANNVIDNKGKTVKSEKMKFTPTSI
                     ALVCIGFTCTSTFVLWLNCNQELGKLYGMADPTKIQSFYSMGVICAVLITSLLIKKYI
                     KPIRILVIYPIIALLMLLVVYFVQTPTICMLGGFVIGYSAAGGVLQLTTSTANEMFPT
                     NKGKITSIVMIASSIANYVILNIAGIITKSGGVNGPKYVVLFNVAITFVGILLALFVN
                     MRYEKEKVYDYDV"
     gene            5472..7337
                     /locus_tag="L3M60_002799"
     CDS             5472..7337
                     /locus_tag="L3M60_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HBN6227441.1"
                     /translation="MENVFKRLQEFDGYDGYKESFEMNYLCIYENIPLREQVELANNL
                     IDGILNMYKSESNEIYLLEDSNSKSLICYFEIFMKKINTLVKEMIIDEKWLYKLTKEL
                     IYKSKKVEYVKLGLVLSEKYLDVENLREVVDTFSKSGEYVFYLSNAIKKIEFYNTYLF
                     NLSKKATGSIKVFAIVNMENLDSKINSYLIEYGYKDTKYQRLLMNYIISIVDLNEYLE
                     KRDLDREKINNLSLLICNYLLSVEFKYIGNKLELVNRFLPIVVNYGTNFESLYSIFLI
                     AINVLKDENIECNKVEFEKEINDILLSEKWKSIYFEALKDASGKTEDMIKMSEIYNVN
                     LSFDDLLPYLNRDIRDFEVYWHISKKGTTSSRLKLLDFFEKTFKVDDLIGKMKDIEKD
                     KLTQEYYDDMLFFIVLKGSKSLYPEGKNISLKGIFGNINEVRKESINILKRYREKLSL
                     EELKVVKEAYEKEKNIILKDELRRVLYESNNLKKEFVNIEKIKVDEHGKDIYLTSITV
                     AGSRFRNREYLEKELEKSKIYYLIREKDNLYDEKAIKIVGETGYVIGYVPRKENYILS
                     NLLDGGKLLYCRVTEYNLYEDCIYANVYLSYKDVIETVENSLKMVLDKSRIKLIN"
     gene            complement(7515..8879)
                     /locus_tag="L3M60_002800"
     CDS             complement(7515..8879)
                     /locus_tag="L3M60_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6227442.1"
                     /translation="MSQLLIKNAYLITMNADREVYENGSILIENNIIKEVGKFDISIV
                     DKDAEIYDAKGKILMPGLVNTHVHLSQQLGRGLADDVVLLTWLRERIWPYESSFNYED
                     SLISSTACCVELIKSGVTTFLESGGQYVDAMVEAVDKTGLRACLAKSVMDTGDGLPEA
                     WQKTTDEEINTQLDLFHKFNNTSNERIKIWFALRTIFNTTEELLVRTKELADKYNTGI
                     HMHIAEIADEISFVKQNNGVGTVEYLDKLGVLGPNLLAAHTVWLTHKEVDLFRLHDVK
                     VSHDPGSAMKVVLGFASIPEMLEKGISVSIGTDGAPSNNRMDLMRDMYLTSLIHKGRT
                     LNPTVVPAEEVLEMATINGAKCALLEKEIGSLEVGKKADMIILNPDTIHCLPMHNPIG
                     NIVYSMTSENVDSTICDGKWLMKERKVLVVDESELLNKVKEQASKIKDKSDVKIPSKF
                     KIIK"
     gene            complement(9057..10400)
                     /locus_tag="L3M60_002801"
     CDS             complement(9057..10400)
                     /locus_tag="L3M60_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HBN6227443.1"
                     /translation="MVPNTNNVQPGGNNSSLSFLDKMFKFSERGSSLKTEVIGGVTTF
                     LTMAYIVFVNPAILSEAGMDKAALITVTILATAIGTLIFAILGNAPFALAPGMGLNAF
                     FTYSLVIGAGIPWQTGLGVVFLSGCVFFTLAVTGLRKTIADAIPRELAVASSAGIGLF
                     LAFVGLKNMGVIIANPDTIVSLADFTPTVLIALFALILMAILEYKKVKGGILISIVVA
                     TIISVIFGFVELPTAIISTPPSIAPVAFKLDIVGALKFSLIGPIFSFMFIDMFDSLAF
                     LITCCKQMGLEDENGEIKDLGRMLYADVSSTLIGSFLGTSTVTTFGESASGIAAGART
                     GLASIVTAALFLATLIFTPLLGIVPTYAAAPALVMVGVFMFESIKQIDFADSKIAVPA
                     FVTVLLMPLTYSISIGLCFGFISYIIMHVVAKETDKISITLWIIGLLAVVNLVLS"
     regulatory      complement(10473..10574)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
BASE COUNT         4008 a         1352 c         1854 g         3641 t
ORIGIN      
        1 ataaaggggg caaatttaaa ttatgaataa tcaaaaaaat ctcaatattc caattacaat
       61 tagttcttat acattaggga cagaagtttc attccgtgat agagttcgta ttgcaaaaga
      121 agctggattt gatggtattg gattacgtgc tgaaaattat attgatgcaa aaaatgcagg
      181 tgttacagat gaagagatga ttgcaattct taatgaatat gatatgaaag ttacagaagt
      241 agaatatata actcaatggg gaacagaaga agatagaacg gaagctcaaa gagaaaaaga
      301 gaaaaatgtt tatcatatgg ctcacttatt taatgttaaa catataaatt gtggattatt
      361 agagatgata ccagaagaac aaattataac tgcattggga gaattatgtg acagagcagg
      421 tgaacttatt ataggtcttg agtttatgcc atatagtggt gttccagatt tggctactgc
      481 atggcgtgtt gtgaaaggat gtaatcgtga aaatgcgatg cttatattgg atacttggca
      541 ctgggctaga gcaaagcaaa cagcagacga ccttgcacct attccagcag ataagatagt
      601 atcaattcaa gtgtgtgatg ttttagaaaa tccttatgaa aaacttaggg atgaatcact
      661 gcatgaccgt ttggctccag gtgaaggatt tggaaatacg gaggaatttg tgaaaataat
      721 aaaagaacac aatatattac caagagtcat aggggcagaa gtaatatctg attctctaat
      781 atctaaagga ttatctactg ctgctgaaac tgtatttaat gcaactaaaa aagttttgga
      841 taaagcatgg tctgaagtat ctccaaaata aacttataca atataaaaat atacgaagta
      901 ttataatata aaacttttaa atataaaact tgaaaggaag gaaaatctta tgaagattag
      961 ttctatgttt acacctataa gaattggttc aatgacagtt ccaaatcgtt ttgtagttcc
     1021 tcctatggga aacaattttg caaacacaga tggtacttta agtgaaactt ctaaggctta
     1081 ttatattgaa cgtgctttag gtggctttgg attaataact atagaatcta gtgttgtaga
     1141 caaaaaagca aaaggaggtc ctcgtaagcc ttgtttatat gatgatagca caatagatag
     1201 ttttaaaaat gtaatagatg cttgtcatga tgcaggttcg aaggtatcta tacaattaca
     1261 acacgctggt tctgaaggaa atgaaaaaat agcaggtcat ccattaaaag ctgcttctgc
     1321 aatacctgca tcaaacggcc gtaatacacc attagcaata acaacagaag aaatatatga
     1381 acttatagaa tcttatggag atgctgcatt acgtgcacaa aaagctggtg cagatgctgt
     1441 tgaaattcat tgtgctcatg gatatctagt tagtagtttt atatcacaaa ggacaaataa
     1501 gcgtgtagat gagtttggtg gatgttttga aaatagaatg agattacctc gtcttatcat
     1561 tgaaaatata cgtaaaaaag taggcaattc tctagcaata ttatgtcgta taaatagtac
     1621 tgatgatgta ctaggaggaa ttgatgtaca tgatagctca gtaatagcag catatctaga
     1681 agattgtgga atagatggac ttcatgtttc gcgtagtata catatacatg atgaatatat
     1741 gtgggctcct acgactttac atgctggatt cagtgcagaa ttagttactg aaataaaaaa
     1801 agctgttagt ataccggtaa taacggttgg acgttataca gagccacagt ttgctgaatt
     1861 aatggttaga caaggaagat gtgatttagt agcatttggt agacaaagct tagctgaccc
     1921 agaaatgccc aataaagcta aaaatggaaa gctagacgaa atgataccat gtattgcatg
     1981 cttacaagga tgtgtaccaa atatgttcca aggtaaaccc atatcttgtc ttgcaaatcc
     2041 tatattagga catgaagcag agtttaagtc agccgaaatt agtaaagaag ttcttgttgt
     2101 tggtggaggc gttggaggta tgcttgcatc atggatatgt gctaaaagag gtcacaatgt
     2161 aactttagtt gaaaaatctg aagttcttgg tgggcaaatg cgtttagctg catatccccc
     2221 aggaaaagga gatattacta acttagttag aagttatatt tcaaaatgta atcaatatgg
     2281 tgtaaaaata tgtactaaca cagaagctac agtagaactt ataaaagaga aatcacctga
     2341 tgcagttatt atagctactg gggcaactcc acttgtactt cctataccag gaatcaatga
     2401 ttcaggatta atacatgctg tagaccttct tgatggtaaa aaatcttgtg gtaaaaaagt
     2461 acttgttgtt ggtggtggaa tggttggatg tgaagtggca gctttccttg gagaacaaga
     2521 acatgatgtt actgttattg agcttagaga agaagtgggt gcagacgtaa tttcagaaca
     2581 ccgtaaattc cttatgaatg attttgaaaa ttataatata catactgtta ctagtgcaaa
     2641 agttgctaaa ttttatgatg atggtgttga ctatttactt gcagatggta cagagcatga
     2701 tttaaaaggt tttgactcag tagtacttgc aatgggttct cgtaattacg acccattaag
     2761 tgaagttata aaagaacttg taaaagaaac ttatgttgtt ggtgatgcag taaaagctcg
     2821 tagagcatta gatgcaacaa aagaagcttt tgaagtagca ttaaatatat aaataataaa
     2881 taaatttttc tttgtaaata actgaatagg ttatttgcaa agaaaatgat aatattggga
     2941 ggaaattaga gttatggaaa tttcaggtag aacaggatta tttgcattaa taggtacacc
     3001 agttggtcac tctaaatcac cagtgatgta taattatagt tttgaaaaat tagatttaga
     3061 ttatagatat ttagcttttg atattacagt agacaaggta aaagaggcac tttcagcaat
     3121 aaaaactttt aatataaaag gagctaatgt tactatgcca tgtaaatctg ctgttacaga
     3181 atatatggat gaactttctc cagcagctcg tataattgga gcatgtaata caattgtaaa
     3241 tgacaatggg aagttagttg gtcatataac agatggggta ggctatgttc gtaatttaaa
     3301 agaaaacgag gtagaagtta aaggtaaaaa ggttacaatt atgggtgctg gtggtgcagc
     3361 gactgctata caagtacaat gtgcattaga tggagcaaga gaaatatcta tcttcaatgc
     3421 taaggatgat ttttataaaa gagcagagca aacagttgag aatataaaaa aagaagtacc
     3481 aaaatgtatt gttaatctat atgatttaga agatacaaac aaactttatg aggagataga
     3541 atcaagtgat atattgacaa atgcaactct tattggtatg aaaccttatg acaatgaaac
     3601 aaatataaaa gatactagtg ttttgagaaa ggatttagta gtaacagatg ttgtttataa
     3661 tcctagaaaa acaaaaatga ttgaagatgc tgaatctaat ggatgtaagg ctattggtgg
     3721 gttaggaatg ttattatacc aaggagcaga atcttttaat ctatatactg gattagaaat
     3781 gcctgtaaaa gaggttaatg aattgtgttt taaatagaga taaaagtgat tgcttaaaca
     3841 atggaaaaac atatgtattt caatgttttt agtaataaat ttcaaattgt catagtgggt
     3901 tttgcattat agtctagtta aatttgaagt tttaactgac tatgctttaa acccattatg
     3961 gggataagtt tattgtacac aaaaatgtta ataaattaac aaggatagga gaagaactat
     4021 gaacaataaa aaatattatc ctactgccat agcgttgtat ttttcatatt ttttacttgg
     4081 tattggaatt tcaattttag gacaatacaa accagaattt tcatcaatgt ggggggctaa
     4141 aacactatca gatggaacat tggatgtaag tattgtttta gctgttattg ctgcactagg
     4201 attagggaga cttatatcgt atccatttgc aggacctatt tcagataagt atggaagaaa
     4261 agtaagtggt cttataggaa actttctaca tgcaattttc tttgttggaa ttgtattttc
     4321 accaaatttt tatatagcat atatatttgc aataattgga ggggcagcaa attcattctt
     4381 agatacctgt gtaacgcctt cttgtatgga gatatttgct agtttaggaa caatagcaaa
     4441 tatgtttact aaatttacaa tagctttagc acagtttttg cttcctttta taattggatt
     4501 tgttgcagca aattcaatat catttaaagt tatttttata gtaacagcga ttcttattgt
     4561 agtagatgcc attttgatag ctgtgttacc atttccacca gctaataatg ttattgataa
     4621 taaaggcaaa acagttaaat cagagaagat gaaatttaca ccaacaagta tagctcttgt
     4681 ttgtattgga tttacatgta catctacatt tgtattatgg ttaaattgta atcaggaatt
     4741 gggaaaatta tatgggatgg ctgaccctac aaaaattcaa tctttctact caatgggagt
     4801 aatatgtgca gtattaataa cttctttact tattaaaaaa tatattaaac caataagaat
     4861 attagttata tatcctataa tagctttact tatgctatta gttgtatatt ttgttcaaac
     4921 tccaactatt tgtatgctag ggggatttgt aataggttat tcagcagcag gaggtgttct
     4981 tcaattaaca acatcaactg caaatgaaat gtttccaact aataaaggaa aaataacgtc
     5041 tatagttatg attgcatcta gtatagcaaa ctatgttatt ttaaatatag caggtattat
     5101 cacaaaatca ggtggagtta atggaccaaa atatgttgta ttatttaatg tggcaataac
     5161 atttgttgga attttacttg cattgtttgt aaacatgaga tatgaaaaag aaaaagtgta
     5221 tgattatgat gtttaatatt agtataatat atttatagag aaatccaaat ttaggatggg
     5281 tttctattaa aaataattaa aattaataag gatattattt atagttataa gatagggtat
     5341 ttacataatt aataataagg atattactag aatttcaatt tctagtaata tttttttgcg
     5401 tataaatgta aacttggcga aataagtagt ataatcaata taaattacat catgtaaagg
     5461 aggataattt ggtggagaat gtttttaagc gattacagga atttgatggt tatgatggat
     5521 acaaagaatc atttgaaatg aattacctat gtatatatga aaatatacct ttaagagaac
     5581 aagtagagct tgcaaataat ctaatagatg gaattttaaa tatgtataaa agtgaatcta
     5641 atgaaattta tttattggag gatagtaatt ctaagtcttt aatttgttat tttgaaattt
     5701 ttatgaaaaa gataaatact ttggtaaaag aaatgattat tgatgagaaa tggttatata
     5761 aattaactaa agaattaata tataagagta aaaaagttga gtatgtaaaa ctaggattag
     5821 ttttaagtga gaaatatcta gatgtagaaa acttaagaga agttgtagat acattttcta
     5881 aatctggaga atacgtattt tatttgagta atgctattaa aaaaattgaa ttttacaata
     5941 catatttatt taatttatct aaaaaagcta caggttcaat aaaagtattt gctattgtta
     6001 atatggaaaa tttagattct aaaataaatt cctatttaat tgaatatggt tataaagata
     6061 cgaagtacca acgattattg atgaattata ttatttcaat tgtagacttg aatgagtatc
     6121 ttgaaaaacg agatttagat agagaaaaga taaacaattt atcacttctt atatgtaact
     6181 acttgctaag tgtagaattt aaatacatag gaaataaatt agaattagtg aatcgatttt
     6241 tacctattgt agtaaattat gggactaatt ttgaaagttt atattcgatt tttctaatag
     6301 ctataaacgt tttaaaagat gaaaatatag aatgtaataa ggttgaattt gaaaaagaaa
     6361 taaatgatat cttgcttagt gaaaaatgga aaagtatata ttttgaggca ttaaaagatg
     6421 ctagtggaaa aacagaagat atgataaaaa tgtcagaaat ttataatgta aacttatctt
     6481 ttgatgactt attaccatat ttaaatagag acattagaga ttttgaggta tattggcata
     6541 tatctaaaaa aggaacgaca agttctagat taaaattatt ggattttttt gaaaagacat
     6601 ttaaagtaga tgatttaatt ggaaaaatga aagatataga aaaagataaa ttgactcaag
     6661 aatattatga tgatatgtta ttttttattg tgcttaaagg aagtaaatca ctatatcctg
     6721 aaggaaagaa tatatcttta aaaggaatat ttggaaatat aaatgaagta agaaaagaat
     6781 ctataaatat tttaaaaaga tatagagaga aattatctct tgaagaatta aaagttgtta
     6841 aggaagcata tgaaaaagaa aaaaatatca tattgaagga tgaattaaga agagtattat
     6901 atgaaagtaa caacttaaaa aaagaatttg taaatattga aaaaataaaa gtagatgaac
     6961 atggtaagga tatctattta acttcaataa ctgttgctgg ttcaaggttt agaaatagag
     7021 aatatcttga aaaagaattg gagaagagta agatatacta tctaattaga gagaaagata
     7081 atctatatga tgaaaaagct attaaaatag ttggtgaaac tggatatgta ataggctatg
     7141 ttccaagaaa agaaaattat attttgagca atttattaga tggaggaaaa ctattgtatt
     7201 gtagagtaac tgaatataat ttatatgaag attgtattta cgcaaatgtg tacttgagct
     7261 ataaagatgt aattgaaacg gttgaaaact ctttaaagat ggttttagat aagagtagaa
     7321 taaaacttat aaactaagac gtctatatac tgcttaaatg atgtaatcct gattagttaa
     7381 ttattttttc tggaattatg gtgtatttta tgagcaatgg taatgaatag gaaataaaaa
     7441 aacaattgtt ttgtatatat gatgtgtata aatatattta tatattcata tttacaaaat
     7501 aattgaaatt tatgttattt tatgatttta aatttagatg gaattttaac gtctgattta
     7561 tcttttattt tagaagcttg ttccttaact ttatttaata attcagattc atctacaact
     7621 aatacttttc tttccttcat caaccattta ccatcacaaa tagtagaatc tacattttca
     7681 ctagtcatag aataaactat atttccaatt ggattatgca ttggtaaaca atggattgta
     7741 tctggattta aaataatcat atcggctttt tttcctactt caagactacc aatttctttt
     7801 tctaataaag cacattttgc tccatttata gtagccattt ctaagacttc ttcagctggc
     7861 actactgttg ggtttaaagt tctaccttta tgtattaatg atgtaagata catatccctc
     7921 ataaggtcca ttctattatt tgaaggagct ccatcagtac caattgaaac agatattcct
     7981 ttttctaaca tttcaggaat tgatgcaaat cctagaacaa ctttcatagc tgaacctggg
     8041 tcatgagaaa cttttacatc atgtaatcta aataagtcaa cttccttatg agtaagccaa
     8101 actgtgtgag cggctaaaag atttggtcct aatacaccca gtttatcaag atattcaact
     8161 gttccaactc cattgttttg ttttacaaat gaaatttcat cagctatttc agcaatatgc
     8221 atatgtatac ctgtattgta tttatcagct aactctttag ttctaacaag taattcttca
     8281 gttgtattaa aaatagttct taatgcaaac catattttaa ttctctcatt agatgtgtta
     8341 ttaaacttgt ggaataagtc taattgagtg ttgatttcct catcagtagt tttttgccat
     8401 gcctcaggaa gaccatctcc tgtatccata actgatttag ctaaacaagc tctaagtcca
     8461 gttttatcaa cagcttcaac catagcatct acatattgac caccagattc taaaaatgta
     8521 gtgacaccag actttatcaa ttcaacacaa catgcagtag atgaaatcaa tgaatcttca
     8581 taattgaagc tactttcgta tggccaaatt ctttctctaa gccaagttaa aagtacaaca
     8641 tcatctgcaa gaccacgtcc taattgttga gataagtgaa cgtgtgtatt gactaaccct
     8701 ggcattagta tttttccttt agcatcatat atctctgcat ctttatctac aatactaata
     8761 tcaaacttac caacctcttt tataatatta ttttcaataa gtatgcttcc gttttcatat
     8821 acctctctat ctgcattcat agtaattaga tatgcatttt ttataagtaa ttgtgacata
     8881 aacaccactc cttttctatg tatttttata tctatgtatt atgataataa attttctata
     8941 caaagttaat agcaaaatca atttacatat aaagtgattt tgctatttat aaatttaaaa
     9001 taaataagat tttattctat ataatttaaa atattgacct agaactattt aaaatattaa
     9061 cttagaacta agtttacaac tgcaagtaaa cctataatcc aaagtgtaat actgatttta
     9121 tcagtttctt tagctaccac atgcattatt atatatgaaa taaatccaaa gcataaacca
     9181 atacttatac tataagttaa aggcatcaat aagactgtaa caaaagcagg tactgctatt
     9241 ttactatcag caaaatcaat ctgttttatg ctttcaaaca tgaacacacc aaccattaca
     9301 agtgcaggag ctgcagcgta agtaggaact ataccaagta atggtgtaaa tataagtgta
     9361 gctaagaata atgctgctgt tactattgaa gcaagtcctg ttcttgcacc tgctgcaata
     9421 ccagaagcag actctccaaa tgttgtaaca gtactagttc ctaagaatga gcctattaaa
     9481 gtagaagaaa catctgcata caacattctt cctaaatctt ttatttcacc attctcatct
     9541 tcaagaccca tttgtttaca acaagtaata agaaatgcta atgaatcaaa catgtctata
     9601 aacatgaaag agaatatagg tccaattaat gagaatttca atgcacctac aatatctaat
     9661 ttaaaagcaa ctggtgctat actaggaggt gttgagatta ttgctgttgg caactccaca
     9721 aatccaaata taacagatat tattgttgca actactatac ttatcaatat tccaccttta
     9781 acttttttat attctagtat tgccattaag ataagagcaa aaagagctat taaaactgtt
     9841 ggtgtaaaat ctgcaagtga aactatagta tctggattag ctattataac acccatattt
     9901 tttaaaccaa caaaagcaag gaaaagacct atacctgctg aagatgctac tgccaattct
     9961 ctaggaattg cgtctgcaat tgtctttcga agacctgtta ctgcgagtgt aaagaagaca
    10021 catccagata gaaaaactac cccaagacct gtttgccatg ggattcctgc ccctataacc
    10081 aaagagtatg tgaaaaatgc atttaatccc ataccaggag ctagagcaaa tggtgcattt
    10141 cctaaaatcg caaatataag tgtaccaatg gctgtagcta gtatggtaac tgttataagg
    10201 gcggctttat ccattccagc ctcagataat attgctggat ttacgaaaac tatgtatgcc
    10261 attgttaaga atgtagtaac tccacctatt acctctgttt taaggctaga gccacgttcg
    10321 ctgaatttaa acattttgtc taaaaagctt aatgatgaat tatttccacc tggttgtaca
    10381 ttgttagtgt ttggtaccat ttttacctcc aaaaaacttt aagatataaa caaaaaaata
    10441 tatattcata tagatagaat agaacatata ttcaaggttg acattctatt catagaaaac
    10501 ccatttaagg tgagtttgta gaaactatta gaccgtatta ctaatattat atgaacattt
    10561 tctatttgtt tttctgttgt attatatcgt aatattgtat tatttacaat aaatataata
    10621 aaattattcg tttttttgtc gattttataa aaaattaagg ttatttattg gcaaaaatta
    10681 ctgttttaaa taattgaata tgtatgagta taatgtcaat tattctaact tgttttatac
    10741 tattaatgga agtttattat aaatgagttt gtgatataat tataaaagta ctgaatacac
    10801 ggatatttcg taaaaacttt ttaatatatt aaatatgtat attaagttaa tatat
//
LOCUS       DAFNNB010000131        10809 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0133, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000131 DAFNNB010000000
VERSION     DAFNNB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10809
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0133"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            520..1185
                     /locus_tag="L3M60_002802"
     CDS             520..1185
                     /locus_tag="L3M60_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HBN6227444.1"
                     /translation="MIKLIGILIVVIGFILKIDTLFTVLLAGMATGIVAGLDFNQIFT
                     ILGDSFVSNRGVSLFILTLPVIGVLERYGLKQRAVSLIEKLKRLTTGKVLTIYMIARQ
                     IAGALSIRMSGHPQFVRPLVNPMAQAAGLSNSDELKESDEEAIKALSAASENYGNFYG
                     QNLFAGSSGVLLIASTLTQFGYNVTGLNIVKANIIMAVIALVVATIQFTLYDKKLNNS
                     HKK"
     gene            1207..2139
                     /locus_tag="L3M60_002803"
     CDS             1207..2139
                     /locus_tag="L3M60_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HBN6227445.1"
                     /translation="MQNFINISLEIFYALMGFLMIVIAYKSFTTINNNKKYGTSLFWI
                     LISLPFIFGRLIPANIIGIILILSSLLTLSKQVIFAKYEEPNENFGKEHADKLKNKIF
                     IPSLILAFAAVVVAMSLSNFANSSQFAIGVGSIIALISALIITKAKPATSVQDGSRLL
                     QQMGPASMLPQLLVALGALFTQAGVGEVISTMISGVVPADSRLFGVIAYVLGMVIFTM
                     IMGNAFAAFSVITAGIGIPFVLSQGGNPAIIGALALTAGYCGTLLTPMAANFNIVPAA
                     LLECKNDYIVIKYQAPVALVLIIAHILVMYFLGF"
     gene            2160..2801
                     /gene="pcp"
                     /locus_tag="L3M60_002804"
     CDS             2160..2801
                     /gene="pcp"
                     /locus_tag="L3M60_002804"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HBN6227446.1"
                     /translation="MKILLTGFDPFGGEPINPAQEAVERVNNNINGAEIIKITIPTVM
                     TKSVEAIDKAIQKHNPDIVISVGQAGGRFDITPERVAINIDDFRIKDNEGNQVIDTVI
                     KEDGEPAYFSKLPVKAMVKHMNENKIPASVSNTAGTFVCNHVMYGILYMIDKKYPNIK
                     GGFIHIPYMTSQVIDKKNTPFMSLEEIVKGLELAIEACIIYKEDVKEIGGEIS"
     gene            complement(2921..3253)
                     /locus_tag="L3M60_002805"
     CDS             complement(2921..3253)
                     /locus_tag="L3M60_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227447.1"
                     /translation="MSIDCISNTDLSETGFSYTLSLISGKYKMIILYCLVEFEVVRYN
                     ALKRYINTISYKTLSLSLKELEADNLIIRTEYPQIPPKVEYSLSERGKSLIPILDAMC
                     EWGERNRP"
     gene            3519..4139
                     /locus_tag="L3M60_002806"
     CDS             3519..4139
                     /locus_tag="L3M60_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6227448.1"
                     /translation="MKVLLINGSPNQYGCTYTALNEITKVLSKHDIKTEILYLGKEAI
                     PGCISCASCFETGKCILNDKVNELIEDLDNIDGIIIGSPVYFSSATGQLTSFLDRLFF
                     IAGSRMATKLGASVVSCRRGGASATFDQLNKYFSISNMPIVSSQYWNQVHGFTPEDVM
                     KDEEGLQTMRTLGENMAWLLKCISAGKKAGIKEPQYEETIMTNFIK"
     gene            complement(4452..5276)
                     /locus_tag="L3M60_002807"
     CDS             complement(4452..5276)
                     /locus_tag="L3M60_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBN6227449.1"
                     /translation="MDFLKKGISGFTIKVLALIFMTFDHIAAFMPQTMQIPIWFHWVG
                     RISAPLFIFMVVEGFCYTSNRKKYISRLYIWSVIMAIGNQIINNVFSHPEGVIIINNI
                     FSTLFLIAIYLQAIEFIKKFRKEKEIKYFIIGLLMIIIPIVLGIFTVTLLFKATNKII
                     ILFMILVPVPFLVEGGPVWIILGIIFYLCRGKKFSLSICYVLMCIFIFTAMSNGDYSL
                     KNSILQNYQWMMIASLPLMLLYNGEKGKSMKYLFYLYYPIHVYILYILGIYLINGF"
     gene            complement(5287..6690)
                     /locus_tag="L3M60_002808"
     CDS             complement(5287..6690)
                     /locus_tag="L3M60_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227450.1"
                     /translation="MKWKITRNFIFTIVFVVISVVIINIISILYVISTDNFLKVVDSR
                     NNPEEFARSFEKDLYEKDGELKLSKIGADKLEKSNSWIQVLNDLGEEVFGVNVPKDTP
                     QKYTPFQMVNNYKYIETEYVNFVLEKYLNKKHLNIIVGIPSRDVSRIILTYSQNNTKK
                     TLNKIIIIILVIDGMVALGVGYLFSRKLTKPISSVLWSIETMANGNYSLYLKDRGIYE
                     EVFKNINMLADTLRVNEVERKENEELREEWLANITHDIKTPLASIQGYAEIINDKDYE
                     FSEDEIREYAEIIYNKSKYIKDLVDDLNLSTRLKNNTIVLDKKKVNLVGLVRNIIIDI
                     LNDNRYKNRNIEFESNEDLIEVYVDSILFRRAITNLIFNSIVHNSEGTLISVKIVKKD
                     NIEIIIKDSGVGISKNDLKHIFKKYYRGTNTGEMHKGSGLGMAISKEIIEIHKGKIYL
                     NSEIGIGTEIIIEINQN"
     gene            complement(6694..7419)
                     /locus_tag="L3M60_002809"
     CDS             complement(6694..7419)
                     /locus_tag="L3M60_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012450879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227451.1"
                     /translation="MENRVLIIDDEVEILKLLETVLKKEGLSNIYTAKTRKEGLEVFK
                     NINPDLIILDIMLPDGEGYDICKEIRKTSNSPIIFLSAKTEELDKLLGLAIGGDDYVT
                     KPFSPKEVAFRVKAHLRRLSYFSDSQNENTDSDNNEEKTISFGPYILNESRAELIKDG
                     KSIGLSAKELKILSLFANNQNQIISKEKLWDKVWGEDYVGFDNTIMVHIRKIREKLED
                     NPSKPEYILTIKGLGYKLSVKED"
     gene            complement(7531..8304)
                     /locus_tag="L3M60_002810"
     CDS             complement(7531..8304)
                     /locus_tag="L3M60_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227452.1"
                     /translation="MMYLVKIEMIKILKSKSVWIIWLFLLFFGFVLIRKFDVLDTYAD
                     IFYKIEGSIPLIGLIMFIITSGNYIKEYDSNMTGLINTTKNGKKSVVLSKLVANGLIL
                     SIINISFILLVGIRGFSFFDFKNLNEPIHNLWYFGNSSLNLTVIQMYIIVILTTIFAS
                     FVFAQIGLTLSSIFKSAVIPFILGGLIMAIPYFSVGFIPDKAIKFMSVTPNWIMMSQQ
                     IVRYNVPSILIGFSIVISIILMIVLTKITYKNFTSSKRF"
     gene            complement(8310..9197)
                     /locus_tag="L3M60_002811"
     CDS             complement(8310..9197)
                     /locus_tag="L3M60_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227453.1"
                     /translation="MNKLEIKNLSKIYGKKVANDKITVTLENGVYGLLGPNGAGKSTL
                     MKQITTLIKPDEGQIFYNGEDIFKMDDDYRNILGYLPQEFGVYKNFTAKEFLQYIGAL
                     KGLKGKYLSSKIEELLDLVGLYDVRDKSIGKFSGGMKRRVGIAQVLLNDPKIIVLDEP
                     TAGLDPQERARFRNLIAKISRNKIIVLSTHIISDIESVAKETIMIKDGKLLMKGTHKE
                     ILSHMENKVYNVQVKNECEVDDIQSKYKVVSIQSDIDSIVLRIVSEDIPIGDNIQPVQ
                     AKFEDVYMFYFDLEDAREA"
     gene            complement(9210..10232)
                     /locus_tag="L3M60_002812"
     CDS             complement(9210..10232)
                     /locus_tag="L3M60_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227454.1"
                     /translation="MVNWEIKKLIKSKSMFISLGILILILSINIFVKPVLETENYYID
                     KERGRIEDTRNSEEIGNYKFQNKINVLKQLSSQKDMDVFSKKIKSISTNKLYNLIINE
                     YKDVSFWKVFNYRATNSLINVGMLIIIVIIISNIYVDELVFSVRDIILSSKEKKKALN
                     SKIVIAFLVPLTIYSVYLIGIFITTCMQQGMPINGDLEAFRIVDNIATLKCNSSIINY
                     VISNIGIEFLMFEGLAMIALLGSFISKSSITSISFFIFTIVITKIISVIHILPKNLLL
                     AISTVNYYDLIFGFNKIIGNYLGEIIIFKIHIDIVNLLIGVLVAIFIGAMSFCFIIIK
                     SDYISK"
BASE COUNT         3850 a         1580 c         1240 g         4139 t
ORIGIN      
        1 ttttatttct aagtaatatt gctcaaaata gtctttagac tttattaaaa ttttattttt
       61 cttaatgaaa tctaataaaa ctagtactca atatatgttt tgttaatcat aataaaaagg
      121 tagtatctga tgaacttttt agttcattgg acactacctt tttgcatgga atcaaatata
      181 tttttatcat ttcaacatta aaaaatagac ttctctctat tttgagatat tctcttttta
      241 taatataata tttaatttaa aactactatt ttaaatatta ccacatactt aaattataaa
      301 catatcaaaa tcaattcata ttagattaaa atataatctt acttattcaa aatttcttta
      361 cataacgctt atttactttg acgatttaaa acatcttccc tattttttat taaattttat
      421 aataatagtc ctattaacgc aaatactata aaaagagttt aactttatcc attaaataaa
      481 tttaaattaa aacatataga aatggaagga gaacaattta tgataaaact aatcggtata
      541 ttgatagttg taattggatt tatacttaaa attgacacat tatttactgt attattagca
      601 ggaatggcaa caggaattgt tgcaggatta gattttaatc aaatctttac aatcttagga
      661 gattcttttg tctcaaacag aggtgtatca ttattcatat taactttgcc agtaatcggt
      721 gtattagaaa gatatggttt aaaacaaaga gctgtttctt taatagaaaa attaaaaaga
      781 ctcacaacag gaaaagtact tactatatac atgatagcta gacaaatagc tggtgctcta
      841 tcaatcagaa tgagtgggca tcctcaattt gtaagaccac ttgttaatcc tatggcacaa
      901 gctgctggtt taagtaatag tgatgaacta aaagaatcgg atgaagaagc cataaaagca
      961 ttgtctgccg cgtcagaaaa ctatggaaac ttttatggtc aaaacctatt tgcaggtagt
     1021 agtggagtgt tacttatagc atctactctc actcaatttg gatacaatgt aactggtctt
     1081 aatattgtaa aagctaatat tattatggca gtaatagcac ttgtagtagc taccatccag
     1141 tttaccttat atgacaaaaa gttaaataat agtcataaaa aatagattta tttaggagga
     1201 ataaaaatgc aaaattttat aaatatatca ttagaaattt tttacgcact aatgggattt
     1261 ttaatgattg tcatagcata taagtctttt actaccataa acaacaataa aaaatatggt
     1321 acatcattat tttggatact tatatcattg ccattcatat ttggtagatt aattccagct
     1381 aatataatcg gaataatatt aatattatct agtttattaa cactttctaa acaagttata
     1441 tttgcaaagt atgaagaacc aaatgagaat tttggaaaag aacatgcaga taaacttaaa
     1501 aacaaaatat ttataccatc attaatatta gcatttgctg cagtagttgt ggctatgagc
     1561 ctttctaatt ttgctaattc ttcacaattt gcaataggtg taggttcaat aattgcatta
     1621 atatcagccc ttataataac taaggcaaaa cctgctacat cagttcaaga tggttcaaga
     1681 ttacttcaac aaatgggtcc tgctagtatg ttaccacaac ttttagttgc attgggagct
     1741 ttgtttactc aggctggagt cggcgaagtt atttcaacca tgatatctgg tgtagtccct
     1801 gctgatagca gattatttgg tgttatagct tatgtattag gtatggttat atttaccatg
     1861 ataatgggaa atgcatttgc tgcattttca gtcataacgg caggaattgg tattcctttt
     1921 gtattgtctc agggtggaaa tcctgctata attggagcat tagcattaac agcaggatat
     1981 tgtggaactc tacttactcc aatggctgct aattttaata tagttccagc agccttacta
     2041 gaatgtaaaa atgattatat tgttataaaa tatcaagctc cagttgcttt ggtattaatt
     2101 atagcccaca tattagtaat gtatttttta ggattttaat ataattagga ggtaaaataa
     2161 tgaaaatttt gttaacagga tttgacccat ttggtggaga accaataaac ccggctcaag
     2221 aagcagttga aagggtcaat aataatataa atggtgcaga aatcataaag ataactatac
     2281 caacagttat gacaaagtct gttgaagcta tagataaagc tattcaaaag cacaatccag
     2341 atatagtaat atcggttgga caagctggag gtagatttga cattacacct gagagagttg
     2401 ctataaatat agatgatttt agaataaaag ataatgaagg taaccaggtt atagatactg
     2461 ttataaaaga ggatggtgaa cctgcatatt tttcaaaatt acctgtaaaa gccatggtaa
     2521 aacatatgaa tgaaaataaa attccagcta gtgtttcaaa cactgcaggg acatttgttt
     2581 gtaatcatgt gatgtatgga atcctttata tgatagacaa gaaatatcct aatataaaag
     2641 gtggatttat acacatccca tatatgactt cacaagtaat agataaaaaa aatacaccat
     2701 ttatgtcttt agaagaaata gtaaaaggtt tagaattggc tatagaagct tgtattatat
     2761 acaaggaaga tgtaaaggaa attggtggcg aaatttcata ataaattttt tagtattttt
     2821 ataaattaaa aatactaact attaatttaa atatagactg tgagtagatt attcaaacaa
     2881 ttttactcac agtcttttta tagtatctac agatacaaaa ttaaggtcta ttcctctctc
     2941 cccactcaca cattgcatct aaaattggaa tcagagattt tcccctttct gataagctat
     3001 attctacctt aggaggtatt tgaggatatt cagtacgaat aatcaagtta tctgcttcta
     3061 attcttttaa tgataaactc aaagttttat aggagatagt atttatataa cgttttaatg
     3121 cattatatct tacaacttca aattctacaa gacagtaaag aattatcatt ttatatttac
     3181 cactaatcag tgaaagtgta taactaaatc ctgtttcact taaatctgta tttgatatac
     3241 agtctatact catatattac actctccttt tggttagtat tgaacaaaaa tgtgcgtact
     3301 tgtttttaga ataatttata ataaaattat agtgtaagaa taattgaaag tcaattttat
     3361 aataggaggt gtatttacta aaaaaggagt tattttaaat aggattctaa gattagatat
     3421 ttctatataa attgataata ttcatgaaaa tcctatttta taaaaaataa tatctcttaa
     3481 aaaatctatt aaataaaatt cattggagga aatttataat gaaagtttta ttgataaacg
     3541 gtagcccaaa ccaatatgga tgtacttata cagcactgaa tgagattact aaagtattga
     3601 gtaaacacga tattaaaaca gaaatactgt atttaggtaa agaagcaatc ccaggatgta
     3661 ttagttgcgc atcctgtttt gaaactggaa aatgtattct aaatgataaa gttaatgaat
     3721 tgattgaaga tctcgataat attgatggta ttattattgg ttctcctgtt tacttttcta
     3781 gtgcaactgg tcaattaact tcatttcttg accgtctatt tttcatagct ggaagtcgta
     3841 tggctacaaa attaggtgct tcagttgtgt catgtagacg tggtggtgca agtgcaacat
     3901 ttgaccaact aaataaatat ttttcaatca gcaatatgcc tatagtatca tcacagtatt
     3961 ggaatcaggt acatggcttt actccagagg atgttatgaa agatgaagaa ggattacaaa
     4021 ctatgcgaac tctaggagaa aatatggcat ggcttcttaa atgtatatca gcaggtaaaa
     4081 aagcgggaat taaagaacca caatatgaag aaacaattat gactaatttt ataaaataaa
     4141 taaaaaaatg aagtaatatt aattaaaaca ctaatctata aataagcata aatataggat
     4201 aaaaagcaat aaaacagagg tatgaatttt atattaaacc tctgttttat tattctatta
     4261 ataatcaaac cctatatcct atcatatatt tctagataga ttataatata atattctaat
     4321 gttattggaa ttacaataac tgaatttctg ttcataattt taaatattct acaaatctca
     4381 tctaaataac aattttaatt gacctaaaac aatcctcata ttattcattt tgtattaaat
     4441 atcacttaca atcaaaagcc atttattaag tatattccta agatatacaa tatatataca
     4501 tggattggat agtataaata aaataaatac ttcatactct tccctttttc tccattgtaa
     4561 agcaacatta aaggtagaga agcaatcatc atccattggt aattttgtaa aatagaattt
     4621 tttaaactat aatctccatt tgacattgct gtaaatataa atatacacat taaaacataa
     4681 caaatactta aactgaactt tttacctcta cacaaataaa aaataatccc aagtataatc
     4741 caaactggcc caccttctac caaaaatggt actggtacca atatcataaa tagaataata
     4801 atcttattag tggctttaaa aagtaaagtt actgtaaata ttcctaaaac tatgggaata
     4861 attatcatca ataatcctat aataaaatat ttaatttctt tctcttttct aaatttcttt
     4921 ataaactcta ttgcttgtaa ataaattgct attaaaaata atgttgaaaa aatgttattt
     4981 ataattataa ctccttctgg atgtgaaaat acattattaa ttatttgatt tcctatagcc
     5041 ataataacag accaaatata caatctactt atatattttt ttctgttact agtgtaacaa
     5101 aatccttcaa caaccataaa tataaatagt ggtgctgata ttctgcctac ccaatgaaac
     5161 caaattggta tttgcattgt ttgtggcata aatgctgcta tatggtcaaa agtcataaat
     5221 attaatgcta gtaccttaat agtaaaccca cttattcctt tttttaaaaa atccatatca
     5281 tatctcctaa ttttgattta tctctataat aatttctgta cctattccaa tttcactatt
     5341 taaataaatt ttacctttat gaatctctat tatttcttta gaaatagcca taccaagacc
     5401 tgaaccttta tgcatctcac ctgtattagt acccctatag tattttttaa aaatatgttt
     5461 caaatcattt ttgcttattc caactccact atcttttatt ataatttcta tattatcttt
     5521 ttttacaatt tttacactta tcaaagttcc ttcgctatta tgaactattg aattaaatat
     5581 aagattggtt atagctcttc taaataagat tgaatctaca tatacttcaa ttaaatcttc
     5641 attgctttca aactcaatat tcctattttt atatctattg tcatttaaaa tatcaattat
     5701 tatatttctg actaaaccga ctaaatttac ttttttcttg tccaaaacta tagtattatt
     5761 ttttaatcga gtagataaat ttaaatcatc aactaaatct tttatatact tagacttatt
     5821 atatataatt tctgcatact ctcgtatttc atcttctgaa aactcataat ctttgtcatt
     5881 aattatctct gcataccctt gtatagatgc tagaggtgtc tttatatcat gtgttatatt
     5941 tgccagccat tcttctctca actcttcgtt ttcttttctc tcaacttcat ttactcttaa
     6001 agtatcagct aacatgttta tatttttaaa aacttcttca tatatacctc tgtccttaag
     6061 atataaacta taatttccat tagccatagt ctcaatactc catagtacac ttgatattgg
     6121 ttttgtaagt tttctactaa ataaatatcc tacacctaaa gctaccatgc catcaattac
     6181 tagtattata atgataattt tatttaaagt ttttttagtg ttattttgag agtaggttaa
     6241 aattattctt gatacatctc tactaggtat ccctactatt atgtttagat gttttttgtt
     6301 taaatatttt tctaggacaa aattaacata ctctgtttct atatacttgt aattatttac
     6361 catttgaaat ggtgtatatt tttggggagt atcctttggc acatttactc caaaaacctc
     6421 ttctcctaaa tcatttaaaa cctgtatcca ggaatttgat ttttctaatt tgtcagctcc
     6481 aattttggat agtttaagtt ctccatcttt ttcatataaa tctttttcaa aagaacgtgc
     6541 aaattcttca ggattatttc tagaatctac aacttttaaa aaattatcag ttgaaataac
     6601 atacaatata gatataatat ttataattac tactgatatg actacaaaca caattgtaaa
     6661 tataaaattt ctagtaatct tccacttcat attctaatct tcctttactg acaatttata
     6721 tccaagacct tttattgtca gaatatattc aggttttgaa ggattgtcct ctaatttttc
     6781 cctaattttt cttatatgaa ccattatagt attgtcaaac ccaacataat cttctcccca
     6841 cactttatcc catagttttt ctttacttat aatctgattt tgattatttg caaataaaga
     6901 taggattttt aattcctttg cagacaatcc tatagacttt ccatctttta taagttctgc
     6961 cctactttca tttaatatat acgggccaaa tgatatagtt ttttcttcat tattatctga
     7021 atctgtattt tcattttgag aatcactaaa ataacttaat cttctcaaat gagctttaac
     7081 cctaaaagct acttccttag gactaaaagg ttttgtgaca taatcatctc ctccaattgc
     7141 tagtccaagt aatttatcta attcttcagt tttagctgat aaaaatatga ttggagaatt
     7201 ggaggttttt cttatttctt tacaaatatc ataaccttca ccatctggta acataatgtc
     7261 taatataatc aaatcaggat taatattttt aaatacttct aatccttctt ttctagtttt
     7321 tgcagtatat atattactaa gaccctcttt ttttaggaca gtttctaaaa gctttaatat
     7381 ttctacttca tcatctatta ttaaaacacg gttttccata aataacctcc tataataatt
     7441 tacctatata ttagcatata ttttatctaa aattcatata tcttatttta aattaaacta
     7501 agatataaat aaaattacta ttatctttag ttaaaatctt ttgcttgatg taaaattttt
     7561 atatgttatt ttggttaaca ctatcattaa tataattgag attactatag aaaaacctat
     7621 caaaatacta ggtacattat atctaacaat ttgttgactc atcataatcc aatttggagt
     7681 cactgacata aattttatag ctttatctgg tataaaacct acactaaagt atggaattgc
     7741 cattatcagt ccacctagta taaatgggat tacagcagat ttaaatatag atgaaagtgt
     7801 taaacctatt tgtgcaaata caaaagaagc aaaaatagta gtcaatataa caattatata
     7861 catttgtatt actgtaagat ttaaactact atttccaaag taccataagt tatgaatcgg
     7921 ttcatttaaa tttttaaaat caaaaaaact aaagcctcta attcctacta acaatataaa
     7981 agaaatatta ataatggata aaattaaacc attagcaact aattttgata atactactga
     8041 ttttttccca tttttagttg tatttattaa acctgtcata ttggaatcat attctttaat
     8101 gtaatttcca gaagttatta taaacattat caaacctatt agaggtattg agccttctat
     8161 cttgtaaaat atatctgcat aagtatctaa aacatcaaac tttctaatta gaacaaatcc
     8221 aaaaaatagt aaaaacaacc aaattatcca aacactcttt gattttaata tttttatcat
     8281 ttcaatttta actaaataca ttatttctac tacgcctccc ttgcatcttc caagtcaaaa
     8341 taaaacatat atacatcctc gaattttgct tgaactggtt gtatattatc tccaattgga
     8401 atatcctctg aaacaattct aagtactatt gaatcaatat cactttgaat gcttacaacc
     8461 ttatatttag attgtatatc atcaacttca cattcatttt taacttgaac attatatact
     8521 ttattttcca tgtggcttaa tatttctttg tgtgttcctt tcattagaag tttgccatct
     8581 ttaatcatta ttgtttcctt tgcaactgat tctatatcgg aaattatatg agtagaaagt
     8641 actattattt tatttctaga tatttttgct attaaatttc taaatctagc tctttcctgt
     8701 gggtcaagac ctgctgttgg ctcatcaagg actattatct tagggtcgtt taataaaact
     8761 tgtgctatac caactcttct cttcatacct cccgaaaatt ttcctatgga cttatctctc
     8821 acatcataaa gtcctactaa atctaatagt tcttctatct tactacttaa atattttccc
     8881 tttaaacctt ttaaagctcc aatatactgt aaaaattctt ttgctgtaaa atttttataa
     8941 actccaaatt cttgtggcaa atatcccaaa atgtttctat aatcatcatc cattttaaat
     9001 atatcttcac cattataaaa tatctgacct tcatcaggtt ttatcaatgt tgttatttgt
     9061 ttcattaaag tagattttcc tgctccattt ggtcctaaaa gaccataaac tccattttcc
     9121 agagttactg taatcttgtc gtttgctact tttttaccat atatttttga aagatttttt
     9181 atttctaatt tattcatctt aactctcctt tatttactta tataatccga ctttattatt
     9241 ataaaacaaa atgacatagc acctataaat atagccacca aaactcctat taatagatta
     9301 acaatatcta tgtgtatttt aaatattata atctctccta aataatttcc tattatttta
     9361 ttaaatccga atatcaaatc ataataattt acagtagaaa tagctaaaag taaattttta
     9421 ggtaatatgt gaattactga tattatctta gttataacta tagtaaatat aaaaaaactt
     9481 attgatgtta ttgaagattt acttataaat gaacctaata gggctatcat agccaatcct
     9541 tcaaacatta aaaactcgat acctatattt gatattacat aatttattat gctcgaattg
     9601 catttcaaag tagctatgtt atctactatt ctaaaagctt ctaaatctcc attaattggc
     9661 ataccttgtt gcatacatgt tgtaatgaat attccaatta aataaacaga ataaattgtt
     9721 aatggtacta agaaagcaat aactattttt gagtttaatg ccttcttctt ttcttttgat
     9781 gatagaatga tatctcttac tgaaaaaacc agttcatcta cataaatatt tgatataatt
     9841 ataacaatga taattaacat tcctacattt ataagtgaat ttgtagctct atagttgaac
     9901 accttccaaa aactaacatc tttatattca ttaattatta aattataaag tttgttagtt
     9961 gatattgatt taattttttt agaaaataca tccatatctt tttgacttga aagttgtttt
    10021 aaaacattaa tcttattttg aaacttataa ttacctatct cttcactatt tctcgtatct
    10081 tctatacgcc ctctttcttt atctatataa taattttcag tttctaaaac aggtttgaca
    10141 aatatgttga ttgatagtat taatattaat attcctagag atataaacat acttttagat
    10201 ttaatcaatt tttttatttc ccaatttacc aatatactaa gcctcctaaa tcattttgac
    10261 tacacttata tattaaaact taaatcttat atcaatataa tcttaacctt aaataaagct
    10321 taaattattt actttattga aactcatcaa aaatatgctt ttaaatttaa ttttttttaa
    10381 aatagtctaa aatatattta ttttttattg taatctttta gtttatcttt tttataagat
    10441 acctgctata ctttttctaa taagattaag tttagtagtt atatgagaaa tacagatatt
    10501 tatataaagt ttagaataaa ccttttaaca tctataggca tgtttatcat tacagttgta
    10561 tttattattt ttggtatttt tattgagtga aaattttatc tttgtctaaa ctgtaatcat
    10621 tgtttagatt taagtaatga gtttctaaag aatggttctt attgtcctca ttatggaaag
    10681 attattaagt agtaattctt ttgcacattt tatttagtgt taattaataa aatttgctct
    10741 aggcttattt ctatacttct tgttaagtta taaaagtttt aaacatgtta aaaagatata
    10801 aaaaattat
//
LOCUS       DAFNNB010000132        10729 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0135, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000132 DAFNNB010000000
VERSION     DAFNNB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0135"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(495..689)
                     /locus_tag="L3M60_002813"
     CDS             complement(495..689)
                     /locus_tag="L3M60_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227455.1"
                     /translation="MNILIYKYIVNEHRDEADELDRDYGEFKIFNGGKCFSGCGRNFY
                     TLAKFKKMIITCPHYEKNIK"
     gene            complement(1226..1606)
                     /locus_tag="L3M60_002814"
     CDS             complement(1226..1606)
                     /locus_tag="L3M60_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227456.1"
                     /translation="MNKEGSEMLKVEKYFSGSLGINIIEDDPTCRNYLALYCCLVGIE
                     RKGKKVIPRIDSMLSEFGVKSRCKRPHNNKLIRLINVKTGEVKEFKSIDSTACFLGLK
                     QPAVSHILKRKTPSRSGWRAEYIK"
     gene            complement(1599..1856)
                     /locus_tag="L3M60_002815"
     CDS             complement(1599..1856)
                     /locus_tag="L3M60_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227457.1"
                     /translation="MIIIRSQDRLDLMRVNRVEIDSKYVYAVFGEESNVREIGRYKSE
                     ERAIEVLSRIQDAIIAGTKFDIIKKDGVRYYKEKVFEMPVE"
     gene            complement(1843..2076)
                     /locus_tag="L3M60_002816"
     CDS             complement(1843..2076)
                     /locus_tag="L3M60_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227458.1"
                     /translation="MNTKKTKGIYKNGGLKVSRTAICSVCGELLVDGYGDEFTNTKII
                     DLHGREFVIIIYKNCLEELLRKCANGGISNDNN"
     gene            2264..2452
                     /locus_tag="L3M60_002817"
     CDS             2264..2452
                     /locus_tag="L3M60_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227459.1"
                     /translation="MTLQDRIKEERLKLGLNQVELSKKFNMTKQTVSNWESENRIQDT
                     LMLTKLADFLCISRFSLG"
     repeat_region   3167..6097
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=3299..3327
                     /rpt_unit_seq="ctttacataccacatagttaatataaaac"
     gene            complement(6376..6528)
                     /locus_tag="L3M60_002818"
     CDS             complement(6376..6528)
                     /locus_tag="L3M60_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227460.1"
                     /translation="MDNFLQGILASLVASLIVYLTSKLFKKVKSHSGRSDFSFELEIK
                     FKKNKH"
     gene            7048..7290
                     /locus_tag="L3M60_002819"
     CDS             7048..7290
                     /locus_tag="L3M60_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBN6227461.1"
                     /translation="MRNRQGIIKNQTFFEDVKLIDITPNLLKIYVNKIYKKYSLNSAK
                     NSIGFLNPLLHEAYILREIQEDVSKFVITSSKKIHL"
     gene            7356..7901
                     /locus_tag="L3M60_002820"
     CDS             7356..7901
                     /locus_tag="L3M60_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBN6227462.1"
                     /translation="MPIYLMLTLGLHFGEAVSLRWCDVELDEGIINIKQTMIYVREKV
                     TFKSPKTEKSKRILTTPIELINLLKEEKIRQNKLKLQDILKNELDLICLNKYFRPWKQ
                     QMFFRLFNRLLEKNNLRYIKLHELRHTNATLMLLSGTNIKTISERLCHADIKITMNRY
                     SLVLEDIDREASDNLSKILFK"
     gene            8446..9291
                     /locus_tag="L3M60_002821"
     CDS             8446..9291
                     /locus_tag="L3M60_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBN6227463.1"
                     /translation="MNITKRMTLLLTLLIVILITGCSPKESASEVAKKYIDKMNSGDY
                     ETAYSLLSKDSKKKISLENLEDFQNILVSTKRIISYKIGKEKIIKKYKHDGKEYKNVV
                     KLEETFNIKNLLYKKDDSLKVNRYFVVENNKYKLLLYESFKKEFGINSMDLAQLKLND
                     FGLKYFKEVDLILKKAIAINPTDSTLYYAQACNYMNFDGFPDYPDATYNALDSINKSL
                     KYLDKNDSDYKKNASNIYNLKGVILMFHKRFDEAQQDLNKSLYFNKDNEDPKSNLNTI
                     KKHLK"
     gene            complement(9515..9679)
                     /locus_tag="L3M60_002822"
     CDS             complement(9515..9679)
                     /locus_tag="L3M60_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227464.1"
                     /translation="MFGKPKESVRFLDFNDVAEKFGEKKAVAIFDAIAGKKSKEHIIK
                     DIEKLVKNEV"
     gene            complement(10211..10542)
                     /locus_tag="L3M60_002823"
                     /pseudo
     CDS             complement(10211..10542)
                     /locus_tag="L3M60_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435227.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
BASE COUNT         4073 a         1656 c         1037 g         3963 t
ORIGIN      
        1 cttatatttt ttctttttta gtcttttttc aagactttaa ttcaaatata ttagaaatta
       61 ttgctaatat tttatctata atactattaa tcgcttgtaa tagagtttcg actaaaaatg
      121 ctttaaatag tagtctttat actttactgg cattttggtt cagtcctcta tttgcaataa
      181 tattattatt ttggatacat aaccctcaca aatatcttaa tagttaagtt tttctatttt
      241 aagaaggtaa aaagtataat taattaaata ttttttacct tctaattatt ttgttttaaa
      301 ttatattcct tagtcattaa agctctttac cctgttagcc ttctttttac attccttata
      361 acaataaacc tccttagatt tttccttaag ataaaataat ttcccacacc aactgcatct
      421 tcttcttttc atataatcac tttcctttat ttactcaatt actctcatat tcacttttcc
      481 atttttaaaa agttttattt gatatttttc tcataatgtg ggcaagtaat tatcattttt
      541 ttaaacttag ctaatgtata gaaattccta ccacatccac taaaacattt acctccatta
      601 aatattttaa attctccata gtctctatct aactcgtctg cttcatctct atgttcatta
      661 actatatatt tatatatcaa aatattcatt aaaaacacct gcctaatcta tttcgatttc
      721 gactatagct ctacttaaaa gcttcacatt ataatttttt tatattttcc tcctctttct
      781 taaatgctct tgctttctct aaatctgtaa atacatgact atatgtcttt cctttttccc
      841 gttttctagt tgtctaccaa gtaactataa attctgttgt aatcatatta ataattactc
      901 ctttatataa attaaagtaa ttctataaag tttttagttt cattcataaa attgccttta
      961 ctatttatct acataaattt catatattct cgtagccttt actattacaa ttttcacact
     1021 cttttaaatt caatctatat tcataaattc tacctattat aaaactagtc attattaatg
     1081 taatactagt taaaatattc atttttaaca atctcctcat attcttctct agaattatct
     1141 atagcaataa acatatcctt gccattgcca tacaactctt ttgctctctt aattgtgtat
     1201 tcagtccttg aaacttccat tattcctact taatatattc agccctccag ccacttctgc
     1261 ttggagtttt tctttttaat atgtgactaa ctgctggttg ttttaatcct aaaaaacaag
     1321 cagtactatc aatagattta aactctttta cttctccagt tttaacattt attaacctta
     1381 ttagcttatt attatgaggt cttttacacc tacttttcac accaaattca gataacatgc
     1441 tatcaattct cggtattact tttttacctt ttctttcaat tccaactaaa caacaatata
     1501 atgctaaata gttcctacat gtagggtcat cctctataat atttattcct aaagaaccac
     1561 taaaatattt ttcaaccttt aacatttcag acccctcctt attcaactgg catttcaaac
     1621 actttttctt tgtaatatct aaccccatct ttttttataa tgtcaaactt agttcctgca
     1681 atgatagcat cctgtatcct acttaatacc tcaatagctc tttcttctga tttataccta
     1741 cctatttctc taacatttga ttcctctcca aacactgcat atacatattt actatctatt
     1801 tcaactctat taactctcat taaatctaat ctatcttgac ttctaattat tatcattact
     1861 aatcccccca ttagcacatt ttcttaataa ttcttctaaa caattcttat atataataat
     1921 tacaaattct ctaccatgta aatctatgat ctttgtattt gtaaattcat caccataacc
     1981 gtcaactaga agttccccac aaacactaca tatagctgtt ctactcactt ttaaacctcc
     2041 atttttatat atgcctttag tctttttagt attcacctta acacctcttt tctagtcaac
     2101 tttatgttaa ctttatattt atatatcagt ctacttttag tttactatca acacaaattt
     2161 tacatttttt tcgaaaaagt atttatttta ttatatgttt tataaaaaat ataacaaata
     2221 gtttatagtt acttatataa acatcaataa aaggagcaat attatgactc ttcaagatag
     2281 aataaaagaa gaaaggctta aacttggctt aaatcaagtt gaattgtcta aaaaatttaa
     2341 catgactaag caaactgtaa gtaattggga gagcgaaaat agaattcaag atactttaat
     2401 gcttaccaaa ttagctgact ttttgtgtat cagtagattc tctttggggt aaagctaata
     2461 atgagcaaat aaataattat gaaaatttca ttgatgaact agatagttat gacgatgtaa
     2521 aagaattaat aaagatattt cttgatttag atgataaaac taaagataag atgttaaaaa
     2581 ttgctcatgt ctttatagag gaagaaaaat caaagattga taatcaaaaa taattttatt
     2641 taaaagaaga gaaattattc ctcttctttt ttgttgtgta agtttttaac aatttcttta
     2701 tattcattaa acttatattt attaatactc tctaattcac agaaaatatt attcaattct
     2761 aatattaatt tatttgattc atccacacaa ctaccccata taacaaactt acgttcgtaa
     2821 ttttagttat aaatcttctg taatatttat aaattattcc tatttatatc ctttaattgt
     2881 atcttttaaa tcagactatt cagaattgtc tttgataatt atcttacttg cataataata
     2941 cttttgctaa atagatgcaa taaaaacttg taattgtaat aaaaaaatct atttttgtag
     3001 gatattggat gttttttcca tttttataga ttattaatcg tttttttaat tatatttttt
     3061 tattaaatca tctttttata atgattttta taatatacta taaatttaag tttgtaataa
     3121 aaaaagttac aataaataga aacattcttg tactcattgt aacttcttaa attcaccact
     3181 tagttaatat aaagcgtaat tttggaaaaa ataattacag aggtaataca acctttacat
     3241 accacatagt taatataaaa tgaggcgttt ttttctgttc cgactcctgt tcctgtatct
     3301 ttacatacca catagttaat ataaaacaag gctatacagg atgataattt aaaagaaaac
     3361 ttcactttac ataccacata gttaatataa aacagttcag ttggaaatat aattgtagaa
     3421 caatactcta ctttacatac cacatagtta atataaaact cattgatgct aacttaagta
     3481 ttccttgttt ttgtgacttt acataccaca tagttaatat aaaacaaata acttagtatg
     3541 tctaaataca aattttagac ctctttacat accacatagt taatataaaa cttaatgatg
     3601 aaggaaaaga atatgtagta tatgaatatc tttacatacc acatagttaa tataaaacaa
     3661 gttctatata caactgtgca agttctgaat taatctttac ataccacata gttaatataa
     3721 aacttaaccc tcttgataaa acaaggaggt attagatgaa actttacata ccacatagtt
     3781 aatataaaac gataccgaag gtaatttact tgcaatatct aaataccttt acataccaca
     3841 tagttaatat aaaacgcggt aaaatgttag ataaaactgt tacatttaca aactttacat
     3901 accacatagt taatataaaa ctaagtttga acacacttat tttcgacccc tcaatacact
     3961 ttacatacca catagttaat ataaaacttt ttgagtatgg agataaagta aaaattatac
     4021 ttccctttac ataccacata gttaatataa aacttagcgt tgataaagat tctatagtag
     4081 tgagaggtgc tttacatacc acatagttaa tataaaacta aaaattggga tataattcat
     4141 caacaaattt aacatacttt acataccaca tagttaatat aaaacattat ccttcatgtg
     4201 cttagctctt ttatattttt tctttacata ccacatagtt aatataaaac tctagctatc
     4261 cagaaactta taaacctgct ccagattcct ttacatacca catagttaat ataaaacgga
     4321 tatgttgaag gaacacatat gttgcaacaa accactttac ataccacata gttaatataa
     4381 aactagcagg agatgataga ggggtatatc accaggtgat ctttacatac cacatagtta
     4441 atataaaacg tttttcttta ttagataaaa gttctccgtc gaatctcttt acataccaca
     4501 tagttaatat aaaacaatca ttccttttgt ttcatcttta agttttacga cctttacata
     4561 ccacatagtt aatataaaac ttagtaagca taataattgc ttgtgtgatg atttgtttct
     4621 ttacatacca catagttaat ataaaacaaa aagaaactac tttgtatctt tatgttggaa
     4681 tagactttac ataccacata gttaatataa aactacttcg tcttttttga tttgaacgtc
     4741 ttattttctt ctttacatac cacatagtta atataaaacg agattttcat atagccatag
     4801 actgttttag tttttcttta cataccacat agttaatata aaactgcttg tgctgtaggt
     4861 gttccttgtg ctgtcatatc tttacatacc acatagttaa tataaaactt taaataatac
     4921 cttagtagaa ttatcagaga aaataacttt acataccaca tagttaatat aaaactccta
     4981 aaccttctat cttctaaact ctcattactt ttctttacat accacatagt taatataaaa
     5041 catgataata atgtagtgaa acgaatagca aaagagttct ttacatacca catagttaat
     5101 ataaaacgag gcagaaataa ttaaatccac aatagattta gctatacttt acataccaca
     5161 tagttaatat aaaacaaatc cctgcaactg tagttggttg gggttataat gactttacat
     5221 accacatagt taatataaaa ctaatgaagg tagcgttagt accaggacat acactaacct
     5281 ttacatacca catagttaat ataaaaccag tagctaattt acttaatcta taagcatcaa
     5341 cctcctttac ataccacata gttaatataa aactatttaa atctgatgaa tatactgctc
     5401 ttatttgccg ctttacatac cacatagtta atataaaact gtttgaacat gactttgaac
     5461 atgtttgtct ttcttcttta cataccacat agttaatata aaacactaat ttaaaaaacc
     5521 acccacttgt agataatgca gctttacata ccacatagtt aatataaaac caaagtatag
     5581 gtcagcatat aaacctaaag tggaagactt tacataccac atagttaata taaaacgaga
     5641 gtaaagattg tgatatagca tacaaaaatt ggtctttaca taccacatag ttaatataaa
     5701 acttaattga ttgttgagta aatcctaatt cttttatttc tttacatacc acatagttaa
     5761 tataaaacta gtttattgtg tgtaaattct gcttcaaaac ttatttcttt acataccaca
     5821 tagttaatat aaaactccac tccaagaatt aggaatagcc ttgctttcac tctttacata
     5881 ccacatagtt aatataaaac tctatgacct attggtgcta atccaagacc ttctccactt
     5941 tacataccac atagttaata taaaacgctc ctcacctctt aatacgccag aactcaaccc
     6001 tctttacata ccacatagtt aatataaaac ttctttagga atcatcagta tggatgaata
     6061 tcagtctact ttacatacca catagttaat ataaaacccc aaaataaact tagcatttcc
     6121 aacacttaca catatgcaac actctaaaat ttgcagtgag cgagcagtgg tgcaaatgat
     6181 aacacttatc atacatcttc aacgccttat atttcaatgc ttaagcccta ttttattcaa
     6241 aaaatcgaac actgcaaaat ttctatatct ttattatatc ataaaaatat tatttttgaa
     6301 tatctgcacc aatttgtggt ataataaaaa caagaagaac tacaatctat ttagacgtag
     6361 agtgaagttc aaatactaat gtttattctt tttgaacttg atttctagtt caaaactaaa
     6421 gtcactcctg ccagagtggc tttttacttt tttaaataac ttactagtta agtaaactat
     6481 taaactggca actaaacttg ctaatatacc ttgtaaaaaa ttatccatac atattcacct
     6541 cccttctata cgttgggagg attatctttt gtatgaactc cactctataa attgtagatt
     6601 acatcttctt gctgaaaaga ttataacata tatttttaca tattttccct actacattac
     6661 tttttcaaaa attcatctat atatttatat acaactaccc tatcatcaaa tagtatttat
     6721 aacttagatt tgatataata aaaccctatt tatttaatgt atcaagaata cttataaatg
     6781 aagtgtacat atgaatatca aatcagcttt tataagaaaa aaaggtggga aatttcatgt
     6841 atatgtggaa tatgtggaag aagaaaccga caaaaagaaa caaaaaagtt atggaagtta
     6901 ttaaaagaaa aaggatgctg aaaaacattt aattgaaata aaatcaacta tagacaacaa
     6961 taagtttgtt gctccaagtg atgtaacact tgtagaaagg tgctatagat acatagagga
     7021 aaaaaagata atatttctct gtatactttg agaaatagac aaggtataat taaaaatcaa
     7081 accttttttg aagatgtaaa gttaattgat attactccaa atcttctaaa aatatatgtt
     7141 aataaaattt ataaaaaata ttctttaaat tctgcaaaaa attcgattgg tttcttaaat
     7201 cctttattac atgaagctta catacttaga gaaatacaag aagatgtttc taagtttgta
     7261 ataacttcaa gcaaaaaaat acatctgtaa ctaactttta tactaaagaa aaagcacagt
     7321 ttctattaga aaaatgtctt gaaactaact tagctatccc tatttattta atgttaacgc
     7381 ttggattaca ttttggagaa gctgtatcgc ttaggtggtg tgatgttgaa cttgatgaag
     7441 gaattataaa tataaaacaa acaatgattt atgttcgtga aaaagtaact tttaagtcac
     7501 ctaaaacaga gaaatctaaa agaatattaa caacaccaat tgaattaata aatttattaa
     7561 aagaagaaaa aataagacaa aataaactaa aattacaaga tatactaaaa aatgaattag
     7621 atttaatatg cttaaataaa tattttcgtc cttggaaaca acaaatgttc tttagacttt
     7681 ttaacagatt attagaaaaa aacaatctta gatatataaa attacatgag cttagacata
     7741 caaatgcaac tttaatgttg ctttctggaa ctaatataaa aactatttca gaaagattat
     7801 gtcatgcaga tataaaaata actatgaata gatactctct tgttttagaa gatatagaca
     7861 gagaagcatc tgataattta agtaaaatac tatttaaata atgctaagtt ctatgtcagt
     7921 aagatgtcag ttaatcacat atttttttaa ttgtcagtta aatatcaatc aaatatttat
     7981 tactagatag tagcaaaaac tagtatttaa ctgctatcta gtagtattat ataatagcta
     8041 aacataacat acctattaat attactgctt ttagcaattt ttttaataaa catcaatatg
     8101 taaaaattat caataaattt ttacatcctc aattaatata aaaaattact aaagaatata
     8161 ttctttttaa aaataaaata tgagaacatt attcaggttt cttagatgca tctgaagatg
     8221 cataaagagt atgtataatt ggactattat tgaagaagtt aaattagaca aatttaatag
     8281 tagcaattta aaataacaag aaaataaact tataaactag ttatctaatt tgttttttta
     8341 attttattaa atactgttct ttatttatac tttaaagaag atgacttatt caatatttga
     8401 tgctagaata taattaataa aaatttaatt ataggtgatt gtattatgaa tattactaaa
     8461 agaatgactt tactcttaac attattaatt gtaatattaa taacaggatg ttccccaaaa
     8521 gaaagtgcta gtgaagttgc aaaaaaatac attgataaga tgaattctgg tgactatgaa
     8581 acagcatata gtttattaag taaagatagc aaaaaaaaaa ttagtctaga aaatcttgaa
     8641 gattttcaaa atattttagt tagtacaaaa agaataatta gttataaaat aggtaaagaa
     8701 aaaatcataa aaaaatataa gcatgatgga aaagagtata aaaatgttgt taaattagaa
     8761 gaaactttta atataaaaaa ccttctttat aaaaaagatg attctctaaa agtaaataga
     8821 tattttgtgg ttgaaaataa taaatataag ctattattgt atgaaagttt caaaaaagaa
     8881 tttggcatta atagtatgga ccttgctcaa cttaagctta atgattttgg actaaaatac
     8941 tttaaagaag ttgacctcat actaaaaaaa gctattgcta ttaatcctac tgattcaact
     9001 ctttattatg cacaagcatg taattatatg aattttgatg gatttcctga ttatccagat
     9061 gcaacataca atgctttaga ttcaattaat aagtctttaa agtatttaga taaaaatgat
     9121 tctgactaca aaaaaaatgc aagtaatata tataacttaa aaggagttat acttatgttt
     9181 cacaaaagat ttgatgaagc acaacaagat ttaaataaat ccttatactt taataaagat
     9241 aatgaagacc caaaatctaa tctaaataca ataaaaaaac atctaaaata gatatttaat
     9301 ttttgaacct ataaaatatt tttttatata taattcagta ctgaatcata atctctacta
     9361 aactaattac aagtttaaat tataaagact tatttctata taaatgttac tgtataacaa
     9421 taagtttttt atttaaattt taaaatattg cctaaaaatc ataaaaaaga aggtataaag
     9481 agctaccctc aagctcaata taccttataa atatctaaac ttcatttttt actaattttt
     9541 ctatatcttt gataatatgt tctttagatt tttttccagc tatggcatca aaaattgcaa
     9601 cagccttttt ctctccaaac ttttctgcta catcattaaa gtctaaaaat ctaacacttt
     9661 cttttggttt accaaacata taacacctct catacatttt tatatatcta ttataacttc
     9721 tatagattta tttttcacca ataaacgtta gacaaattag tccctttatt tttttagact
     9781 atctttacct tcaaaattcc taacaaaacc agtttattct actctataaa taaaaataga
     9841 atatttaatt tgtagaaaac tgataataga atacaacaag taaaactttg tttaatctat
     9901 ctgcaaaagt catctgttaa aactgctatg aattataaag agtattctag ttcataaaaa
     9961 tacaaatatg caaatataat taccttaata ttcaatttat ttaaaataaa aaatatacaa
    10021 aagatttaat aataacaata tacaattctc aatacaatct atgatgtaaa taattcaaga
    10081 ctactatatc attttaaaat ttatatatgc gattctaaaa acttaataag aaatgaactt
    10141 agaaaataaa acttaatttc aaatacttaa atagactatt attatcctaa ataaactact
    10201 tctatcaaaa ttatatatta aattcttcta aaatttttat tgcaaatctt gcgtttggat
    10261 atgtaataga cccaccacca attacaccta tttttatagc ttctataatt tcatctttac
    10321 ttgctccaga attaacacaa ccctcaatat gataacaaat atactcatca catcgactta
    10381 aaattgaaat agctagcctc attaattcct tattttttta cttatagccc cttctgaata
    10441 agtcttatca tcaaggcttc caaatactct aaaaaactca tttgtttcgc ttaatttttt
    10501 attgtaaaat cccctatatt ctttcatttc tctagtattc atcttctacc tactatgttt
    10561 ttcttataag aaaatcacac tgtgcttata ttttcaacac taaatacaaa ttttcaattt
    10621 atgttcatta gcctaactta tattattttt tgaaatctca acaagttatc atataagtaa
    10681 gattttttca gaactaaaat agaaatctta gtaatttctt tattatcta
//
LOCUS       DAFNNB010000133        10589 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0136, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000133 DAFNNB010000000
VERSION     DAFNNB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0136"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(91..789)
                     /locus_tag="L3M60_002824"
     CDS             complement(91..789)
                     /locus_tag="L3M60_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6227465.1"
                     /translation="MEKKYFKIHGVPSILWGNPSSKLYIYIHGQCGNKEEAEAFANIA
                     VPQECQVLSVDLPEHGERKGEKNSFDPWHVVPELVSIMEYAKCHWNQISLYANSIGAW
                     FSMLSFEKENLRECIFVSPILDMQQLISNMMLWANVSEEQLKHELIIPTSFGHTLSWE
                     YLQYVKQHPIIQWNVPTKILYGKHDELTEIGVVEKFVEKFNCNLTIMEDGKHWFNTPQ
                     ELNSLYEWLNTLVK"
     gene            1047..1604
                     /locus_tag="L3M60_002825"
     CDS             1047..1604
                     /locus_tag="L3M60_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227466.1"
                     /translation="MRVSREIVIQATSDIVNKDGLNKVSLKVIAENLGIRTPSLYNHI
                     ESLDDLLLEVAHKGMREMNNQMTKAVIGNFGDTAIKCISIAYFNFIISNPGVYETIQW
                     ATWHGNNETLEIFDEYKSLLEKLILSCNLNTKNTGEVLNLIMSVLHGYSTLQLGKSLM
                     SKEEAIKGLTDSIDTVLLGIHKKYD"
     gene            complement(1985..2458)
                     /locus_tag="L3M60_002826"
     CDS             complement(1985..2458)
                     /locus_tag="L3M60_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227467.1"
                     /translation="MFNEHLIRELKEDDIKDALELIWNVFEEFESPDYCDEGIMTFHQ
                     FIEYNSIIEKVKEKTLFFWGCFDCEKLIGVLAIRDLNHICMLFVDKNYHKRGVAKKLM
                     TIGIEYCICNKFSLTEITVNSSPYAVEVYHKLGFKDTAVEQISDGIRFTPMKLLI"
     gene            2751..3518
                     /locus_tag="L3M60_002827"
     CDS             2751..3518
                     /locus_tag="L3M60_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaP domain-containing protein"
                     /protein_id="HBN6227468.1"
                     /translation="MQIIDININNIDNEHICCSISEKKGENCVFSKKLWMKNNFDKGL
                     VFKRFDVRGKAFIEYIPAENAWCPIIADNYMFINCFWISGKFKGKGYSNLLLQECVDD
                     AKLKGKYGLVVLSSSKKMPFLSDAKYLKYKRFRLADTSVPNYELLYFPFTDNTPIPCF
                     KNCVKQRKLNENGIVLYYSNQCPHTEKYALILKKIANSRGIDANLIKFETANEAQNAP
                     APFTAYSLFFNGEFVTNEVLSEKKIEKFLENEVKKFN"
     misc_binding    3737..3950
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4196..5230
                     /locus_tag="L3M60_002828"
     CDS             4196..5230
                     /locus_tag="L3M60_002828"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBN6227469.1"
                     /translation="MSKIKVGIIGATGYVGAELIRLLINHDKAELTAIGSNSYVGKDI
                     VDIYPSIGYKNNMICIENEKVIDVCDVVFTALPHGVSEKFVIKAIKSKKKVIDLGADF
                     RIKDEEVYSKWYGVSFIDKILHKKAVYGLSEIYKDDIKDADIIANPGCYPTSISLPLM
                     PLLSSKLIKSNNIIIDSKSGLTGAGRELSISSHFTEVNENITAYKIGKHRHTPEIEQN
                     LSESCQEKVSVVFTPNLIPVNRGILSTIYCTKADNISINDIHRKLTDYYEFKEFIEVL
                     PLDKVASLKNVRFSNKCVISLHENVDTLIICSAIDNMIKGAAGQAIQNMNIMFGIEEN
                     TGLKNIVPSF"
     gene            5271..6488
                     /gene="argJ"
                     /locus_tag="L3M60_002829"
     CDS             5271..6488
                     /gene="argJ"
                     /locus_tag="L3M60_002829"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HBN6227470.1"
                     /translation="MEIIKGGVTVSEGFFASGIHCGLRKNKEKRDLALVYSDVLCDAA
                     AVVTQNKVKGNPVYVTQEHIKNGKAQAIIVNSANANTFNGKEGLINAYKMAKFTSDKL
                     KIKESDVLVASTGVIGKPLNIDLIEENMDELVNNLSKQGHIGAREAIMTTDIIKKEIA
                     VAIMYGDKKITIGGMAKGSGMIHPNMATTLGFITTDANIDGVLLKEALKIAVDKSFNR
                     VSVDGDTSTNDMVMILANGKAKNDRINKKDEHYQVFLSALTYVCIELAKLVAKDGEGC
                     TKLIECYINGALSEEHAVKLAKTVISSSLVKTAVFGADANWGRILCALGYAGEEIDME
                     KVDIIFESMKGYIEVCKNGNGLDFNEEKAKKILEDDEISILVDLNMGNARGNAWGCDL
                     SYDYVRINGSYRN"
     gene            6503..7363
                     /gene="argB"
                     /locus_tag="L3M60_002830"
     CDS             6503..7363
                     /gene="argB"
                     /locus_tag="L3M60_002830"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBN6227471.1"
                     /translation="MINIEKANTLIEALPYIEKHQGKTIVVKYGGSAMKKDGLKESVM
                     EDLVLMSYVGINIVLVHGGGAEINKMLAKVDIESKFVNGLRYTDEETMEIVKMVLAGK
                     VNKDLVNKIHTKGGKAVGLCGIDNNMILCDPYKNYELGFVGEIKKVNVELIESCLKSG
                     YISVIATIGVGDDGETYNINGDTAASAIAKELNADKLILLTDVPGLLREPDEEKSLIT
                     EVILEDVDKLFKEGIITGGMIPKIQGCVDALNNGVNRVHILDGRVPHSIITELFTDSG
                     IGTLIRKENE"
     gene            7393..8580
                     /locus_tag="L3M60_002831"
     CDS             7393..8580
                     /locus_tag="L3M60_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HBN6227472.1"
                     /translation="MNNNSTISKWTEYFIDTYNQPNFVIDYGEGSCFFDTNGNKYIDF
                     TSGYGVSSLGYSNSNLKNALKEQVDKLLHTSNLYFNEPVLYAGEKIINASGMDKIYFC
                     NSGTEANETAFKIARKYSSDKYGNGRGTIISLKDSFHGRTMMSLMATGMDKYHKYFYP
                     LPEGFKYIERNNIEDLKNNLDSTVCAIILEAIQGEGGVNVLEKDYVLELVKICQEKDI
                     VVIFDEVQCGIGRTGKLFGYEYFDVKPDIVTVAKGLGAGIPVGGVLVNKKLSKVLGKG
                     DQGTTFGGNLLAMVAASVVLDEISKDGFYNEVLEKGNYIRKSIESFNNKVVLKIKGIG
                     LMIGIETNIESSIIEEKARKKGLLILTAGKNVLRFLPPLTISYEEIDEALEILKDILL
                     EIN"
     gene            8690..9712
                     /gene="argF"
                     /locus_tag="L3M60_002832"
     CDS             8690..9712
                     /gene="argF"
                     /locus_tag="L3M60_002832"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBN6227473.1"
                     /translation="MNNISLKGKSFLTLKDFTKEEIRYLLTLSSYLKTKKKVGIKGDL
                     LQGKNIALLFEKTSTRTRCSFEVAAHDEGAHVTYLGPNDCHMGKKESVADTAKVLGRF
                     YDGIEFRGFKQETVESLAKYSGVPVWNGLTDEYHPTQILADFLTVIENVDKDLNKVKF
                     VYVGDARNNMGNSLMIGCAKMGIHFVALAPKELWPNEELVKEMKEIAKKSKGGIMLTE
                     NIADVKGADVIYTDVWVSMGEEEQYETRINQLKYYQVNMDMIKKTENENVIFLHCLPA
                     FHDLETKVAQEIYEKFGLSELEVTDEVFNSKYSKVFEEAENRMHTIKAVMVSTMTDVN
                     MNNNLI"
     gene            9839..10456
                     /locus_tag="L3M60_002833"
     CDS             9839..10456
                     /locus_tag="L3M60_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4878 domain-containing protein"
                     /protein_id="HBN6227474.1"
                     /translation="MKKIILALTTFLLAISLTACSSAKPEDTINSFFNSAKKFDFEGM
                     NKVMENNDEKYNDILKELDTKDPNEQYVLDYLKQNASKITYTIKDSEVKGDKATIKVE
                     CKFIDSTPLLQEIVAEAFTKMLGMSFSGQDLTDEKTIEMLVSIMKEKQKSVKETYVTK
                     TVEFECSKKDNKWIISSVNDAVADVLLSNLVTAGQEFSNSINALE"
BASE COUNT         4230 a         1076 c         1822 g         3461 t
ORIGIN      
        1 aataataact aatttgttct ttatgtagtt attttaataa tactaaaata aaattaatta
       61 aataacattt ataaataatt taattatttt ttatttaact aaagtattta accattcata
      121 taatgaattt aattcttggg gtgtgttaaa ccaatgtttg ccatcttcca taatagtaag
      181 gttgcaatta aacttttcta caaatttctc tacaacacca atttcagtaa gctcatcatg
      241 tttaccatat aatatttttg ttggtacatt ccattggatg ataggatgtt gttttacata
      301 ttgtagatat tcccatgaaa gagtatgccc aaatgaagtt gggataatta gttcatgttt
      361 aagctgttct tctgatacat ttgcccaaag catcatattt gaaataagct gttgcatatc
      421 tagaattgga gatacaaaaa tacactctct taagttctct ttttcaaagc ttaacatact
      481 aaaccatgct ccaatgctat ttgcatataa cgaaatttga ttccagtggc actttgcata
      541 ttccataata gatactagtt ctggaacaac atgccatggg tcaaatgaat ttttttctcc
      601 tttacgctct ccatgttcag gtaaatcaac acttaaaact tgacactcct gtgggactgc
      661 aatattagca aaagcttcag cctcctcttt attgccacat tgtccatgta tgtaaatata
      721 taatttactt gaaggatttc cccaaagaat tgaaggaact ccatgaattt tgaaatattt
      781 tttttccata aaaatctcct ctcagtttga tatattagtg attgttaaat taatcaaact
      841 ataaaataat gttgtaatat ataatttaat ataatactaa ttatattagt attaaagcta
      901 atttaattag tattatatta aattataatt aaactgtcaa gattttataa tagtataaag
      961 aattttataa attacaataa ttgacgaaac taattagatt agttaaacta ataaagtaat
     1021 taattttaaa tgaaagaggg aaaatcatgc gtgttagtag agaaatagtt atacaagcta
     1081 catcagatat agttaataaa gatgggctta acaaggtttc tttaaaagtt attgcagaaa
     1141 atctaggaat acgtacacca tctttgtaca atcatataga aagtctagat gatttattac
     1201 ttgaagtagc tcataaagga atgagagaga tgaataatca aatgacaaag gcagttattg
     1261 gaaattttgg agatacagct ataaagtgta taagtatagc atattttaat tttataattt
     1321 ctaatcctgg tgtttatgaa acaattcagt gggctacttg gcatggaaat aatgaaacat
     1381 tagaaatctt tgatgaatat aaatccttat tagaaaaact tattctttct tgcaatctaa
     1441 atacaaagaa tacaggtgaa gtattaaact taataatgag tgtacttcat ggttattcga
     1501 ctttgcaact tgggaaatca cttatgagta aagaagaagc tataaaaggt ttaactgatt
     1561 ctattgatac tgtattatta ggtattcata aaaaatacga ttaagtaggt aaatcaacta
     1621 ttttaacaca tttattgaac aaattttcat gaggcatata aattataatt ccatgataaa
     1681 aaattttacc atttgtaaca aaggaaaagc tacaggatag aagtatattg caataatgtt
     1741 tattttttaa tggacaatca ttcttacaaa taggagatat atatggaaac aacataagtt
     1801 aagcttaata attgaattga tttatatatt agtcaaaaaa atatcaaaaa ataaatcaga
     1861 gttccatatt agtaaggctt ttaaggtatg ttataaaaaa aataaaaaag atagtgctat
     1921 ttttaaaatt taatttaaaa gcaaagtcac cataaagcat tcaagagact ttgcttttta
     1981 tttactatat taacaatttc ataggtgtaa aacgtatacc atcagaaatt tgctcaactg
     2041 cagtatcttt aaaccctaat ttgtgataga cctcaacagc ataaggtgat gaatttacag
     2101 ttatttcagt taaactaaat ttattacaaa tacagtattc tatgcctata gtcattaatt
     2161 tttttgcaac accacgttta tgatagtttt tatctacaaa taacatacat atatgattta
     2221 aatctctaat agctaagaca ccaatcagtt tttcacagtc gaaacaaccc caaaagaata
     2281 gtgttttttc tttcactttt tcaataatag aattatattc tataaattga tgaaatgtca
     2341 tgattccttc atcacaataa tctggagatt caaattcttc aaatacattc cagattaatt
     2401 ctaatgcatc tttaatatca tcttctttta attctcttat taagtgttca ttgaacatta
     2461 taaacaacac ctctattatt aaaaatttta attataatta taattatata ccttaaataa
     2521 atttaattca atttgtttta taattctgga acattattat aacatttaat ttatacattg
     2581 tagttaggtc atagaatgta tttaattaca tataaaattt gataattcaa ataattttaa
     2641 aataatatta tgtgaaataa aaatatatat gacataatgt tgttgtattc aaaatgttaa
     2701 aataaagaat atatttaaaa attatcatgt aattagaaaa ggagataagt atgcagatta
     2761 ttgatataaa tataaataat attgacaatg aacatatatg ttgttcaatt tctgaaaaaa
     2821 aaggagaaaa ttgtgttttt tctaaaaaac tttggatgaa aaataatttt gataaaggtc
     2881 tagtttttaa acgatttgat gtaagaggta aagcttttat agaatatatt ccagcagaaa
     2941 atgcatggtg tcctattatt gcagataatt atatgtttat caactgtttt tggatatctg
     3001 ggaaatttaa gggaaaagga tattctaatc tattgctaca agagtgtgtt gatgatgcaa
     3061 aattaaaagg aaaatatgga ttagttgtgc tttcgtctag taaaaaaatg ccttttttat
     3121 ctgatgctaa atatctaaaa tataagagat ttcgtttagc agatacatca gtgccaaatt
     3181 atgagctatt atattttcca tttactgata atactccaat tccatgtttt aaaaattgtg
     3241 taaaacaaag aaaattaaat gagaatggta tagttttata ttatagtaat caatgtccac
     3301 atacagaaaa atatgctctt attttgaaaa aaattgcaaa ttctagaggt attgatgcaa
     3361 atcttataaa atttgaaaca gcaaacgaag cacaaaatgc tcctgcacca tttactgcat
     3421 acagtctttt ttttaatggt gaatttgtaa ctaatgaggt actttctgag aaaaagattg
     3481 agaaattttt agaaaatgaa gtaaaaaaat ttaactaaaa ggagatataa atatactatt
     3541 ttgaaattat attattttat aatttaatgt attttgaaag tttcttaagt tgactaatga
     3601 taatataaaa cttatatcac aatttgaatg caaaatggtt aaagttaacc atatagaaaa
     3661 tatttccaaa aatttataaa ttatattgac aaacttaaaa atataaacta ttatataaaa
     3721 caataagaaa ataaattgaa atcctgtgaa aaagagagta gcataaaact aattgataga
     3781 gagctgggaa tggtggaagc ctagtaatga attttattgt gaatagagct ttggaggaga
     3841 ttaccagaag taatagtatg gtaattggtg ggtctcgcct taagagatta gagtgggtaa
     3901 gattatctta tcaattagag tggtaacgcg ggtgtatatt cgtctcttgg tctttgacca
     3961 agaggctttt ttaattttaa gtatacattt gtagatactt tttaatttga gttttaaaga
     4021 ctattataat tttgccaata agagtggtac cgcggaaaat cgcctcttag tatttataat
     4081 actgagaggt tttttgtttt aattggaata ttttaatctg tgtattatca aatgattatc
     4141 atataataaa aataaaattc tgagactatt taaatataaa aatggaggta atcaaatgag
     4201 taaaataaaa gttggtatta ttggtgcaac tggatatgtt ggggcagaac tgattagatt
     4261 attaataaat catgataaag ccgaattgac agcaataggt tcaaattcgt atgttggaaa
     4321 agatatagtt gatatatatc caagtatagg atataaaaat aacatgatat gtatagaaaa
     4381 tgaaaaagtt atagatgtgt gtgatgttgt gtttacagct cttccacatg gagttagtga
     4441 aaagtttgta ataaaagcaa ttaaatcgaa aaagaaggtt atagatttag gtgctgactt
     4501 tagaataaaa gatgaagaag tgtatagtaa atggtatgga gtatccttta tagataaaat
     4561 cttacataaa aaagctgtat atggattaag tgaaatatac aaagatgata taaaagatgc
     4621 agatataatt gcaaatcctg gatgttatcc aactagtatt tctttaccat taatgccatt
     4681 gctaagttct aaattgataa aaagcaataa tataataatt gattctaaat caggtcttac
     4741 aggtgctggt agagaattat cgattagtag tcactttacg gaggttaatg aaaatatcac
     4801 tgcatataaa atagggaaac acagacatac tccagaaata gaacaaaatt taagtgaatc
     4861 ctgccaagaa aaagtaagtg tagtttttac accaaattta ataccagtaa ataggggaat
     4921 attatcaact atatattgca caaaagcaga taatatttct ataaatgata tacatagaaa
     4981 attaactgac tattatgaat ttaaagaatt tatagaagtt ctcccattag ataaagtagc
     5041 ttctttaaag aatgttaggt tctcaaataa atgtgtaata tcattacatg aaaatgtaga
     5101 tacattaata atctgttcag ccatagataa tatgattaag ggagcagcag gtcaagctat
     5161 acaaaacatg aatataatgt ttggtattga ggaaaataca ggtttaaaaa atattgtacc
     5221 atcattttag aaataaaaag tactaaactc atataattag gaggataaat atggagatta
     5281 taaagggtgg agttacagtt tcagaaggat tttttgcttc agggattcac tgtggcttaa
     5341 gaaaaaataa agaaaaaaga gatttagctt tggtttattc agatgtttta tgtgatgcag
     5401 cagcagtagt tactcaaaat aaagtaaagg gaaatcctgt ttatgttaca caggagcaca
     5461 ttaaaaatgg aaaagcacaa gctatcatag taaatagtgc taatgctaat acctttaatg
     5521 gaaaagaagg acttataaat gcttataaaa tggccaaatt taccagtgat aaattaaaga
     5581 ttaaagaaag tgatgttttg gttgcatcta caggtgttat aggaaagcca ttaaacatag
     5641 atttaataga agaaaatatg gatgaattag tgaataatct aagtaaacaa ggtcatattg
     5701 gagcaagaga agctattatg acaactgata ttataaaaaa agaaatagca gtagcaataa
     5761 tgtatggaga taaaaaaata actattggag gtatggcaaa aggttctggt atgatacatc
     5821 ctaatatggc aacaacgtta ggatttataa ctacagatgc aaatattgat ggagttttac
     5881 taaaagaagc tttgaaaatt gctgttgata aaagttttaa tagagttagt gtagatggag
     5941 atacaagtac taatgatatg gtaatgattc tagcaaatgg aaaagcaaaa aatgatagaa
     6001 taaacaaaaa agatgaacat tatcaagtat ttttgagtgc acttacatat gtttgtatag
     6061 aacttgctaa acttgttgca aaagatggag aaggatgcac aaaactaatt gaatgttata
     6121 taaatggagc attaagtgaa gaacatgcag taaaattagc aaaaacagtt atatcttctt
     6181 cattagtaaa gacagctgta tttggagctg atgcaaattg gggtagaata ttgtgtgctt
     6241 taggttatgc aggtgaagaa atagatatgg aaaaagttga tattattttt gagagtatga
     6301 aaggatatat tgaagtatgt aaaaatggaa atggactaga ttttaatgag gaaaaagcaa
     6361 aaaaaattct tgaagatgac gaaataagta tattagtaga tttaaatatg ggcaatgcca
     6421 gaggaaatgc atggggatgt gatttaagct atgactatgt aagaataaat ggaagttata
     6481 gaaattagga gggtatactt taatgattaa tatagaaaaa gcaaatacac ttatagaagc
     6541 tttaccatat atagaaaaac atcagggaaa gactattgta gttaagtatg gtggaagtgc
     6601 aatgaaaaaa gatggtctaa aggaatctgt tatggaagac cttgtgttaa tgagttatgt
     6661 tggaatcaat atagttttag tacatggtgg aggggcagaa atcaataaga tgttagcaaa
     6721 agtggatata gaaagtaaat ttgtaaatgg tcttagatat acagatgaag agacaatgga
     6781 aattgtaaaa atggtacttg ctggaaaggt taataaagat ctagtaaata aaatacatac
     6841 aaaaggtgga aaagcagtag ggctttgtgg aatagataat aatatgattt tatgtgaccc
     6901 atataaaaac tatgaacttg gatttgtagg agaaatcaaa aaagtaaatg ttgagcttat
     6961 agaatcttgt ctaaagtcag gttatatttc agtaattgca actataggtg ttggagatga
     7021 tggagaaact tataatataa atggtgacac agcagcttct gctatagcta aagagttaaa
     7081 tgcagataaa ttgatacttc ttacagatgt acctggcttg ttaagagagc cagatgaaga
     7141 aaaatcatta ataacagaag ttatattaga ggatgtcgat aaattattta aagaaggtat
     7201 aattactgga ggtatgatac ctaaaataca gggatgtgta gatgccttaa ataacggagt
     7261 aaatagggta catattttgg atggaagagt tccccattct ataataacag agctatttac
     7321 tgatagtgga ataggtacat taataagaaa agaaaatgaa taaaattata agtttaagaa
     7381 gggagaataa gaatgaataa taacagtaca atttcaaaat ggactgaata ttttatagat
     7441 acatataacc aacctaattt tgttatagat tatggggaag gaagttgttt ttttgatact
     7501 aatggcaata aatatataga ttttacaagt ggatatggag tatcttctct tgggtatagt
     7561 aacagtaatt taaagaatgc tctaaaagag caagtagata aattattaca tacttcaaat
     7621 ttatatttta atgagccagt gctgtatgca ggggagaaaa taattaatgc aagtggaatg
     7681 gataaaatat atttttgtaa ttctggaaca gaagcaaatg aaacagcatt caaaatagca
     7741 agaaagtata gtagtgataa atatggtaat ggtagaggaa ctattataag tttaaaagac
     7801 tcttttcatg gtagaacgat gatgtcttta atggcaactg gtatggataa ataccataag
     7861 tacttttatc cacttcccga aggttttaaa tatatagaaa gaaataatat tgaagattta
     7921 aaaaataatc ttgattcgac agtttgtgca ataattcttg aagcgattca aggtgaagga
     7981 ggagttaatg ttcttgaaaa agattatgtt ttggaactag taaaaatatg tcaagaaaag
     8041 gatattgtag tgatttttga tgaagtacaa tgtggtatag gaagaactgg caaacttttt
     8101 ggatatgaat attttgatgt aaaaccagat atagttactg tagctaaagg gctgggagca
     8161 gggataccag taggaggagt attggtaaat aaaaaacttt caaaagtact aggaaaagga
     8221 gaccaaggta caacttttgg aggtaatcta ttagctatgg tggcagcttc agttgtatta
     8281 gatgagatat ctaaggatgg tttttataat gaagtattag aaaaaggaaa ttatataaga
     8341 aaatctattg aaagttttaa taataaagtt gtattaaaga taaaaggtat tggtctaatg
     8401 ataggaattg aaactaatat tgaatctagt attatagaag agaaagctag aaaaaaagga
     8461 ttgcttattt tgactgctgg aaaaaatgta ttgagatttt taccaccatt aacaatcagt
     8521 tatgaagaaa tagatgaggc tcttgaaatc ctaaaagata tattactaga aattaactaa
     8581 atattcaaaa aatagtttga ttaaagataa aaattagaag taaataaaaa agtaaagtgc
     8641 taacaagttt aattgaattt aaaaagttta taaaaagggg cgaattaata tgaataatat
     8701 aagtttaaaa gggaagagtt ttttgacatt aaaggatttt acaaaagaag agattagata
     8761 tttattaact ttatcaagtt atttaaaaac taagaaaaaa gttggtataa aaggcgattt
     8821 gctacaagga aaaaatatag ctttattatt tgagaaaaca tcaacaagaa ctagatgttc
     8881 atttgaagta gcagctcatg atgaaggagc acatgtaact tatctaggtc caaatgattg
     8941 tcatatgggt aaaaaagagt ctgtagcaga tacagcaaaa gttctaggaa gattctatga
     9001 tgggatagaa tttagaggct ttaaacaaga aacagttgaa agcctagcaa aatattctgg
     9061 tgtacctgtt tggaatggac ttacggatga atatcatcca acacaaatac ttgcagactt
     9121 tttaacagta attgaaaatg tagataaaga tttgaataaa gttaaatttg tatatgttgg
     9181 tgatgctaga aataatatgg gaaactcact gatgatagga tgtgctaaaa tgggaatcca
     9241 tttcgtagca ctagcaccta aggagctttg gccaaatgaa gagttggtta aggaaatgaa
     9301 ggaaatagcc aagaaatcta agggaggcat aatgttgact gaaaatattg ctgatgttaa
     9361 aggtgcagat gttatatata cagatgtatg ggtttctatg ggagaagagg agcaatatga
     9421 aacaagaata aatcaactta agtattatca agtaaatatg gatatgataa agaaaactga
     9481 aaatgaaaat gttatatttt tacactgtct tccagcattt catgatttag aaactaaggt
     9541 tgctcaggag atttatgaaa agtttggatt atcagaatta gaggttacag acgaggtatt
     9601 taattcgaaa tattcaaagg tatttgaaga agctgaaaat agaatgcata caataaaagc
     9661 tgttatggtt tctactatga ctgatgtaaa tatgaacaat aatttaatat agatattcct
     9721 taatttagat tttgttttaa taaaaattta taaaatattt taaatatatt attcaaaaac
     9781 tatcattata tgttaaaata gcttatgtat caacaaatta agagggagga tttgagatat
     9841 gaaaaaaatc atattagctt taacaacttt tttattagca atatctttaa cagcatgttc
     9901 aagtgcaaaa ccagaagata caatcaatag tttttttaac agtgcaaaga agtttgactt
     9961 tgaaggcatg aataaggtaa tggaaaataa tgatgaaaaa tataatgata tattaaagga
    10021 attagatacg aaagacccaa atgaacaata tgttttagat tatctaaaac agaatgcgtc
    10081 taaaatcaca tatacaataa aagacagtga agttaaaggt gataaggcta caatcaaagt
    10141 agaatgtaaa tttatagatt caacaccgtt gcttcaagaa atagtagctg aggcatttac
    10201 aaagatgctt ggtatgagtt tttcaggaca agacttaact gatgagaaaa caattgaaat
    10261 gttagtatca attatgaaag aaaaacaaaa aagtgttaaa gaaacttatg tgacaaaaac
    10321 tgtagagttt gaatgttcta aaaaggataa taaatggata ataagttcag taaatgatgc
    10381 agtagctgat gtattgttat ctaatcttgt tacagcagga caagaatttt ctaactctat
    10441 aaatgcactt gaataatatt gtttaggtgt agtaaagtaa taaacatata tttatttatg
    10501 aaggatacca tttttggtat cctttttatt aatataatga aatacatggt taatttctat
    10561 attgtcaaga aactatgata tatgttata
//
LOCUS       DAFNNB010000134        10494 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0137, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000134 DAFNNB010000000
VERSION     DAFNNB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10494
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0137"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            8..2470
                     /gene="recQ"
                     /locus_tag="L3M60_002834"
     CDS             8..2470
                     /gene="recQ"
                     /locus_tag="L3M60_002834"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HBN6227475.1"
                     /translation="MLSKYYGYTSFRKGQESIINSILNEKDVLAIMPTGGGKSICYQI
                     PALILDGMTIVISPLISLMKDQVDALKAMGISAAFINSSLSSKEFNEILNNIRKNNYK
                     ILYIAPERLDAQEFLELINNNNVSQVAIDEAHCVSQWGHDFRLSYRRISDFIKSLPKR
                     PIVTAFTATASEEVRIDIINLLCLENPDYYITGFDRENLSINIVKSSSKNKYILDYIQ
                     NHKNESGIIYVATRKEVENIYNGLSKRNISVSKYHAGLSKNERSKNQEDFINDNVDIM
                     VATNAFGMGIDKPNIRWVIHYNMPQSIENYYQEIGRAGRDGEKSECVLLFSPGDVHIQ
                     KYLIDIGVENPERKLFQHKKLQYMIDLVYSNSCYRKTILNYFGENYMDNCNNCSNCLV
                     EGEIVDKTIDAQKVISCVARMKRSYGVTTIVDVLRGSKNKKILQLGLNTLSTYNIMRD
                     YSSEDLKNFINTLVSHGFLDLVETLGNGNRGSFPTIRLNEMSMKVLKGEVKVEFKEIV
                     MTKSLEVEDELYNVLRELRHSIASEERIAPYMVFGDGTLRAMSSSYPINKEEMLDISG
                     VGEIKYQKYGRDFETVIKEYVEKNNINKKINEKNVDLKNVNSDFLEVNTDKVLYEKLL
                     VIREEYATKEKLPEYMILANKTLKEISGRYPLDEEQLKDISGIGGVKFEKYGASILEE
                     VNKYVKENDINVIWEKKGKRKLILDGESRKNNEIALDLLNQGKDIQSISQEIEVSLST
                     IIGYVHDYVKDGHRISFELGLENFYNEDKKKMILEVCEKVGYDNLNNIKRKLPDSIKY
                     ENIRAVILDSYLENLKNNIIAE"
     regulatory      2559..2670
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2812..4113
                     /gene="thiC"
                     /locus_tag="L3M60_002835"
     CDS             2812..4113
                     /gene="thiC"
                     /locus_tag="L3M60_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBN6227476.1"
                     /translation="MNYTTQMDAARKGIITKEMEIVSQKEQVDVNELRELIANGQVVI
                     PANKNHKSLSAEGVGKNLRTKINVNLGISRDCKDIEKELEKVRVAIDMKAEAIMDLSN
                     YGKTREFREKVVEMSPAMIGSVPMYDAVGYLEKELKDITEEEFLNVIRQHAIDGVDFI
                     TIHAGLTRSVCQKVKNHERLTHIVSRGGSLLFAWMELNNKENPIYTNFDKILDICEEY
                     DVTLSLGDACRPGCIKDSTDGVQIQELVVLGELTKRAWERNVQVMIEGPGHMAIDEIE
                     ANVVLEKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGALACAKGVDFLCYVTPAEHL
                     RLPNLDDMKEGIIAAKIAAHAGDIAKNVKGAREWDNKMSKARADLDWCEMFRLAIDPE
                     KAKRYRDESTPTHEDSCTMCGKMCSMRTVKKILNNEELNLI"
     gene            4167..4361
                     /gene="thiS"
                     /locus_tag="L3M60_002836"
     CDS             4167..4361
                     /gene="thiS"
                     /locus_tag="L3M60_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBN6227477.1"
                     /translation="MKVNGKEIEFEENLTVIDLLNKYNLKSDRVVVEVNLEIIEESNY
                     NTYVLKDEDIVELISFIGGG"
     gene            4354..5157
                     /gene="thiF"
                     /locus_tag="L3M60_002837"
     CDS             4354..5157
                     /gene="thiF"
                     /locus_tag="L3M60_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HBN6227478.1"
                     /translation="MVKIKIFVNESLTDVEENTTAYKVRDSYNNKCDVLVLNGYPIKT
                     DMPLCENDKLTLIQKGIKPSLDELESLLIARHTPNIHNKLKKGKVAILGLGGLGSNIA
                     ISLARIGVGELLLIDYDVVEPSNLNRQQYFIDDIGKLKTDAMIENIKKINPFIKLNKR
                     DVFLNKNNMDTIKDSDLIIEAFDDASCKAQVCNYVLINLKDKYLIASSGMAGYYDSNI
                     ITTKKIKDRFYICGDFVNEAKFGEGLMAPRVAICANHMANLATQILIDM"
     gene            5220..5990
                     /locus_tag="L3M60_002838"
     CDS             5220..5990
                     /locus_tag="L3M60_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBN6227479.1"
                     /translation="MDKLVLGGHEFNSRLLVGTGKYGSNNILPEVIKESGSEIITMAL
                     RRVDLDNKQENILTYIPKEMTILPNTSGATNAEEAVRIARISRKMGCGDFIKIEVISD
                     TRYLLPDNEETIKATKILADEGFIVLPYMTPDLYAGRRLIEANAAAVMPLGAPIGSNR
                     GLQMKEMIRIMIDELDIPIIVDAGIGKPSQAMEAMEMGADAVLVNTAIASAGDPIQMA
                     RAFKLAVEGGREAYIAKTGSVSEFANASSPLTGFLGNL"
     gene            6007..7110
                     /gene="thiH"
                     /locus_tag="L3M60_002839"
     CDS             6007..7110
                     /gene="thiH"
                     /locus_tag="L3M60_002839"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBN6227480.1"
                     /translation="MSFYDVIEKYRNFDFDGYLNNVTDNDVLRSLSKDKLEDFDILNL
                     LSKTAVKHLEAMAQKAHKLSVQYFGKTVCLYTPMYIANYCVNQCVYCSYNIKSGIKRK
                     KLTMDEIREEGEAISKEGFKHLLVLTGESSFHSSVEYIGEAIEILREKFPSIGIEVYP
                     MEVEEYKYIVDKGVEGLTVYQETYDEEIYKRVHIKGPKSNYKFRLDAPERGAKAGMRT
                     LSIGALLGLNDFRKETFFTILHGKYLKTKYPYIELSYSTPRMRPFKGCFEELVDISDT
                     DLVQAMVCMRLFDPHAAINISTRENLEMRSHIIPLGVTKLSAGVSTDVGGHSQDEHDT
                     AQFKINDESTVKDVEKMLNSIGYQHVFKDWERF"
     gene            7111..7800
                     /locus_tag="L3M60_002840"
     CDS             7111..7800
                     /locus_tag="L3M60_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBN6227481.1"
                     /translation="MYLITNRKLCSEERYLEVIKESILSGVENIIIREKDLEYQELKN
                     LYMKIKTKINCIDFQEQISDESLKTNINQKGCGNKFKVNFIINSNIEFFEKMDCQGIH
                     LPFKLFLNLIENKYNFNENKILGLSLHKVDEVEYLEKLIRKQNIKIDYITLSHIYETK
                     CKEGLNPKGIELLKEAKKITDIKIIALGGILPSNVKETLKYCDDFAVMSTIMKSKDIK
                     KTISNYNEKLN"
     gene            7986..9380
                     /gene="yfcC"
                     /locus_tag="L3M60_002841"
     CDS             7986..9380
                     /gene="yfcC"
                     /locus_tag="L3M60_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBN6227482.1"
                     /translation="MTDSKKKRFVIPHTFTIIFLLIVLMAILTWIVPSGEFSREDVEG
                     RMVVVPGTYQSVESNPQGFADVFKAPIEGFIDSAEVVGFVLIVGGAFGIVNRTGAIEA
                     GIASVISKFKGKELLIIPISMILFGLGGTTFGMAEETLPFYMIFVPLMTSLGYDSLTA
                     IAIVFIGAAAGAAASTINPFSVGIAQALAQLAPGSGVTYRVVIFIAYILISIAFVMYY
                     AKKVKENPESSIVYEIDKNNKSILNQTSTEIKEFTFKEVSVLAIFGIGMAIMIWGVLS
                     QGWYIPEISMCFAAIGVLSGLAGRLSQIEISESFIDGAKDLASAALAIALARGIVIIA
                     QNGFIIDTILNSAAAFLGGLPKVIFVYLSFFLEAALAFLIPSSSGLASLTVPVLAPLG
                     DLVNISAQVIVTAYQFGVGLTNFITPTSGVLMGALAVAHIPFSKWVRFIMPILLILTA
                     VSLVFLTIGIYIGF"
     gene            9896..>10494
                     /locus_tag="L3M60_002842"
     CDS             9896..>10494
                     /locus_tag="L3M60_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBN6227483.1"
                     /translation="MGLKEFDFIEESIDMLRTMSPTLETISDEIEEYFENILDEKNQE
                     YINVTSRIKSESSLREKIIRNRYLKKYGEASNLIHNVSDLIGLRIECRFIEDENKIYR
                     LLRRYFNKTDDKINYYNKENKNIKLKLSERQPNKQKNGFEIYKIDGVFAYLDREVKFE
                     LQIKSLVNVFWSEIEHKIIYKNNTYLLADKFIKDMMDSI"
BASE COUNT         4189 a         1043 c         1945 g         3317 t
ORIGIN      
        1 tttagaaata ctatcaaaat attatggata tacaagtttt agaaaaggtc aggaatctat
       61 aataaattct atcttaaacg aaaaagatgt attagctatt atgccaacag gagggggaaa
      121 atcaatatgt tatcagattc cagctttgat tttagatgga atgacaatag taatttctcc
      181 acttatatct cttatgaaag accaagtaga tgcattaaaa gctatgggaa ttagtgctgc
      241 ttttataaat agttcattaa gtagcaaaga atttaatgaa atattaaaca atattagaaa
      301 aaataattat aaaatactct atattgctcc agagagatta gatgcacagg agtttcttga
      361 gctaataaat aataacaatg taagtcaagt tgcaatagac gaagcacatt gtgtatctca
      421 atggggacat gattttaggt tgagttatag aaggatttct gattttataa aaagtttgcc
      481 taaaagacct atagttacag catttactgc aacagcttca gaagaggtaa gaattgatat
      541 aataaattta ctgtgtttag aaaacccaga ttattatata actggatttg atagagagaa
      601 tttatcgata aatatagtaa aatcatctag taaaaataag tatattttag attatattca
      661 aaaccataaa aatgagtctg gaattatata tgttgcaact aggaaggaag ttgaaaatat
      721 atataatggt ttatctaaga gaaatatttc tgtgtcaaaa tatcatgcag gtctatctaa
      781 aaacgaaaga agtaaaaatc aagaagactt tataaatgat aatgtggata taatggtagc
      841 aacaaatgca tttggaatgg gtattgataa acctaatata agatgggtta tacattacaa
      901 tatgcctcaa agtatagaaa attactatca agaaataggt agagctggta gggatggaga
      961 aaaaagtgag tgtgtacttt tattttctcc aggagatgta catatacaaa aatatttaat
     1021 tgatattgga gttgaaaatc ctgaaagaaa attatttcag cataagaagc ttcaatatat
     1081 gattgactta gtttacagta attcatgcta tagaaagaca atattgaatt attttggaga
     1141 aaattatatg gacaattgca ataattgtag taattgttta gtagagggtg aaatagtaga
     1201 taaaactata gatgcacaaa aagtcatttc atgtgtagct agaatgaaaa ggagttatgg
     1261 tgttactact attgtagatg tacttagagg ttctaaaaat aaaaagattt tacagcttgg
     1321 actaaatact ttgtctacat ataatattat gagagattat tctagtgaag atttaaagaa
     1381 ttttataaat acattagtat ctcatggttt tttagattta gttgagactc taggtaatgg
     1441 aaatagaggt agttttccaa ctataagatt aaatgaaatg tctatgaaag tgttaaaagg
     1501 tgaagtgaaa gtagaattta aagagattgt gatgacaaaa tcattggaag tagaagatga
     1561 gttatataat gttttaagag aacttagaca ttcaattgca agtgaagaaa ggatagctcc
     1621 atatatggta tttggagatg gcacgcttag agccatgtct agtagttatc caattaataa
     1681 agaagaaatg ctagatatct ctggagtagg agaaattaaa tatcaaaaat atggtagaga
     1741 ttttgaaaca gtaattaaag aatatgtcga aaaaaataat ataaataaaa aaataaatga
     1801 aaaaaatgtc gatttaaaaa acgttaatag tgatttccta gaggttaata cagataaagt
     1861 tctatatgaa aaactgttag tcattagaga ggaatatgca acgaaggaaa aattacctga
     1921 atatatgatt ttagctaata agactttgaa agaaataagt ggaaggtatc cacttgatga
     1981 agaacaatta aaagatattt ctggaatagg tggggtaaag tttgaaaagt atggagctag
     2041 tattttagaa gaggttaata aatatgtaaa agaaaatgat attaatgtta tttgggagaa
     2101 aaaggggaaa aggaagttaa tattagatgg agaaagtaga aaaaataatg aaatagcctt
     2161 agatttactg aatcaaggaa aagacataca aagtataagt caagaaatag aagtttcact
     2221 gtcaaccatt attggatatg tgcatgacta tgtaaaggat ggacatagaa tatcatttga
     2281 attagggtta gaaaattttt ataatgaaga taaaaagaag atgattttag aagtttgcga
     2341 aaaagtaggt tatgataatt taaataacat aaaaagaaaa cttccagatt caataaagta
     2401 tgaaaatata agagctgtga tacttgactc ttatcttgaa aacttaaaaa ataatattat
     2461 agcagagtaa tttttaactt aataaaaaat aaaatttgta ttattatgtt gaactaatat
     2521 ttttgctgtg ttatactgaa tttgtaaaaa taatgaaaaa ttagagctag gggcgctaga
     2581 attggctagc tgagagataa agccgtaact tacaaaccct aataacctga tcaagataat
     2641 gcttgcgaag ggaagctttt taatataatt ttattatata aataggcaaa ttatttaatg
     2701 ttagggtaaa tttgtctaag tgtatatacg attataagag tgctttccaa acaaggaaaa
     2761 gcactttttt tattttcaaa aactataact ttaatcttgg aggattgtaa tatgaactat
     2821 acaacacaaa tggatgctgc tagaaaaggc ataataacta aagaaatgga aatcgtttct
     2881 caaaaagaac aggtagatgt aaatgaatta agagaattaa tagcaaatgg acaagttgtt
     2941 ataccagcca ataaaaatca taaatcttta agtgcagaag gtgtaggtaa aaatttaaga
     3001 acaaaaataa atgtaaatct aggaatttca agagactgta aggatataga aaaagaactt
     3061 gaaaaggtta gagtagcaat agacatgaaa gctgaagcta taatggattt aagtaattat
     3121 ggtaaaacta gagaatttag agaaaaagtt gttgaaatgt cacctgcaat gataggttca
     3181 gtacctatgt atgatgcagt aggatatttg gaaaaagaat taaaagatat aacagaagaa
     3241 gagtttttaa atgtaataag acaacatgct attgatggag tagactttat tactatacat
     3301 gcaggtctta caagaagtgt ttgccaaaaa gtaaaaaatc atgagcgatt aactcatata
     3361 gtatctcgtg gaggttcttt attgtttgca tggatggaat taaataataa ggaaaatcct
     3421 atttatacta attttgataa aatattggat atatgtgagg aatatgatgt tacattgagt
     3481 ttaggagatg cttgtagacc aggttgtata aaagattcta ctgacggtgt tcaaattcaa
     3541 gaacttgtag tattaggtga acttactaaa agagcctggg aaagaaatgt acaagtaatg
     3601 atagaaggtc ctggacacat ggctattgat gaaatagaag caaatgtagt attagaaaag
     3661 agattatgcc atggagcacc tttttatgtt cttggaccat tagttacaga tatagcacca
     3721 ggttatgacc atataacaag tgcaatagga ggagctttag catgtgctaa aggagtggat
     3781 tttttatgtt atgtaactcc agcagagcat ttaagactgc ctaatttaga tgatatgaaa
     3841 gaaggtataa tagctgcaaa gatagctgct catgcaggag atatagctaa aaatgttaaa
     3901 ggtgctcgtg aatgggataa taagatgagt aaagctagag ctgatttaga ctggtgcgag
     3961 atgtttaggc ttgcaataga ccctgaaaag gctaagagat acagagatga atcaactcct
     4021 actcatgaag atagttgtac tatgtgtgga aaaatgtgct caatgagaac ggttaagaaa
     4081 atattaaaca atgaggaact taatttaata taattgttgg atagtatgac taaagtaaaa
     4141 agaagttgaa aagaggaggt gatttagtga aagttaatgg taaggaaatt gaatttgaag
     4201 aaaatttgac tgttatagat ttattaaata aatataattt aaagagtgat agagttgtag
     4261 ttgaagtgaa tttagagata attgaagaat ctaactacaa tacatatgta ttaaaagacg
     4321 aggatattgt agagcttatt agctttattg gaggtggtta aaataaaaat atttgtaaat
     4381 gaatcattaa cagatgttga ggaaaataca actgcttata aagttagaga tagctataac
     4441 aataaatgtg atgtattagt gttaaatgga tatccaatta aaacagatat gcccctttgt
     4501 gaaaatgata aattaacact tatacaaaaa ggcattaagc catcacttga tgagttggaa
     4561 agtttgctga ttgctagaca tactccaaat atacataata agttaaaaaa agggaaggtt
     4621 gctatattgg gattgggagg acttggttcc aatatagcta tctctttagc tagaattgga
     4681 gtgggagaac ttttacttat agattatgat gttgtagaac catctaactt gaatagacaa
     4741 cagtatttta tagatgatat aggaaaacta aaaactgatg ccatgataga aaacataaaa
     4801 aaaataaatc cttttataaa attaaataaa cgagatgttt ttttaaacaa aaataatatg
     4861 gacactatta aagactcaga tttaataatt gaggcatttg atgatgcttc atgtaaggca
     4921 caggtatgta attatgtatt aataaattta aaagataaat atctaatagc atcatctggt
     4981 atggcaggtt attatgattc aaatataata actactaaga agataaaaga caggttttat
     5041 atatgtgggg actttgtgaa tgaagctaaa tttggtgaag gacttatggc tccaagggtt
     5101 gctatctgtg caaatcatat ggcaaatttg gcaacacaaa tacttataga tatgtaaata
     5161 ttaaaattag acactaaaaa gattgaatat taaaaaaatt taatatggag ggaaataata
     5221 tggataaatt ggtacttggt ggacatgaat ttaatagcag attattggtt ggaacaggta
     5281 aatatggttc aaataatata ttgcctgagg taataaaaga aagtggaagt gaaataataa
     5341 caatggcact tagaagagtg gatttagaca ataagcaaga aaacatatta acatatattc
     5401 caaaagaaat gacaatactt ccaaatacat caggagctac taatgcagaa gaagctgtaa
     5461 gaatagctag aatatctaga aaaatgggat gtggagattt tataaaaata gaagtaatat
     5521 cagatactag atatctactt cctgataatg aagaaactat aaaggctaca aagatacttg
     5581 ctgatgaggg gtttatagtt cttccgtata tgacaccaga cctttatgct ggaagaaggc
     5641 ttatagaagc taatgcagca gcagttatgc cacttggagc tccaattggt tcaaatagag
     5701 gtcttcaaat gaaagagatg ataagaataa tgatagatga gttggatata ccaataatag
     5761 tagatgcagg tattggaaaa ccatctcaag ctatggaggc tatggaaatg ggagctgatg
     5821 cagtacttgt caatactgct atagcttcag caggagaccc tatacaaatg gctagagcat
     5881 ttaaattagc tgtagaaggt ggaagagaag catatatcgc aaaaacagga agtgtgtcag
     5941 aatttgcaaa tgcttcttct cctttaacag gttttttagg caatttatag aaagtggtga
     6001 ttggtgatga gtttttatga tgtaatagaa aaatatagga attttgattt tgatggatat
     6061 ttaaataatg ttactgataa tgatgtttta agaagtttat caaaagacaa gcttgaggat
     6121 tttgatatat taaatttgct ttctaaaact gcagtaaaac atttagaagc catggcacaa
     6181 aaagcacata aattaagtgt tcaatacttt ggtaaaacag tatgtttata tacgccaatg
     6241 tatatagcaa attattgtgt taatcaatgt gtttattgta gctataatat aaaaagtgga
     6301 ataaaaagaa aaaaattaac catggatgaa attagagaag agggagaagc aatttcaaaa
     6361 gaaggtttta aacatctact tgtcttgact ggagaaagta gttttcactc aagtgtagaa
     6421 tatataggtg aggctataga aatattaaga gaaaaatttc cctctatagg gatagaagtt
     6481 tatccaatgg aagtagaaga atataagtac attgtagata aaggtgttga aggactaaca
     6541 gtatatcaag aaacttatga cgaggaaata tataagagag tacatataaa agggccaaaa
     6601 tcaaattata aatttagact agatgctcct gaaagagggg caaaagcagg tatgaggact
     6661 ttatcaatag gtgcactttt aggattaaat gattttagaa aagaaacttt ttttacaata
     6721 ttgcatggga aatacttaaa aacaaaatat ccttatattg agctttcata ttcaacacca
     6781 agaatgagac cttttaaagg ttgttttgag gaattggtag atataagtga tactgattta
     6841 gttcaagcta tggtatgtat gagattattt gaccctcatg cagctataaa tatatcaact
     6901 agagaaaatt tagaaatgag aagtcatata atacctttgg gagtaactaa attgagtgca
     6961 ggtgtatcta ctgatgtagg aggtcattct caagatgaac atgatacagc tcaatttaag
     7021 ataaatgatg aaagtactgt taaagatgtg gagaaaatgc taaactcaat tggttatcag
     7081 catgtgttta aagattggga aagattttaa atgtatttga taacaaacag aaagttatgt
     7141 agtgaagaac gatatttaga agttattaaa gagtctatat tgagtggtgt agaaaatatc
     7201 ataataagag aaaaggattt agaatatcaa gagctaaaaa atttatatat gaaaataaaa
     7261 actaaaatca attgtataga ttttcaagag caaatatcag atgaaagttt gaaaactaat
     7321 ataaatcaaa aagggtgtgg aaataaattc aaggttaatt ttataataaa tagtaacatt
     7381 gaattttttg aaaaaatgga ttgtcaaggt atccatcttc cttttaaatt attcttgaat
     7441 ttaattgaaa ataagtataa ttttaatgaa aataaaatac taggattatc tttacacaaa
     7501 gtagatgaag tagaatattt agaaaaatta ataagaaagc aaaatataaa aatagattac
     7561 ataacattat cacatatata tgaaactaaa tgtaaagaag gattaaatcc aaaaggaatt
     7621 gagcttttaa aagaagctaa aaagattact gatattaaaa taattgcact tgggggaatt
     7681 ttaccatcta atgtcaaaga aacattaaaa tactgtgatg attttgctgt aatgtcgact
     7741 ataatgaagt ctaaagatat taaaaaaacc atatcaaatt ataatgaaaa gttaaattag
     7801 taaatatagt atgttgaatt tttaacttaa ataaaaataa gaaagctatc ttttaaattt
     7861 taaaaggtag ctttttattt tgtatctttg ctaataaaaa attgtaaaaa gggaaaactt
     7921 tagtcagaga gattaacaat atcctagaaa gatttaaaat ttagtaaagt atggaggaca
     7981 ttataatgac tgacagtaaa aagaaacgat ttgttatacc acacactttt acaataatat
     8041 ttttactaat agtcttaatg gcaatattga cttggatagt accaagtggt gaatttagta
     8101 gagaagatgt agaaggcaga atggtagttg taccagggac ttatcaaagt gtagaaagca
     8161 atccacaagg atttgcagat gtttttaaag caccaataga aggatttatc gattcagctg
     8221 aagttgtagg ttttgtttta atagttggag gtgcttttgg tattgtaaat aggactggag
     8281 ctatagaagc aggaatagct tcggttatct ctaagtttaa gggaaaagaa cttcttataa
     8341 ttccaatatc aatgatttta tttggacttg gaggtacaac atttgggatg gcagaagaga
     8401 cattaccatt ttatatgata tttgttccac ttatgacatc cttgggatat gattctctaa
     8461 cagcgatagc aattgtattt ataggagcag cagcaggagc cgcagcttca acaataaatc
     8521 cattttcagt tggtattgca caggcactag ctcaacttgc tcctggttca ggagtaactt
     8581 atagagtagt tatttttata gcatatatac taataagtat agcatttgtc atgtactatg
     8641 ctaagaaggt aaaggaaaat cctgaaagtt caatagtata tgaaatagat aaaaataata
     8701 aatcaatact taatcaaact tcaacagaaa taaaagaatt tacttttaaa gaagtatctg
     8761 ttcttgctat atttggaata gggatggcaa taatgatttg gggtgtttta agtcaagggt
     8821 ggtacattcc agaaatatca atgtgttttg ctgcgatagg tgtgctttct gggctggctg
     8881 gtagattatc tcaaattgaa atttcagaaa gttttataga tggagcaaaa gatttagcat
     8941 cagcagctct tgccatagct ttagctcgtg gaatcgtaat aattgctcaa aatggattta
     9001 taatagatac tatattaaat tccgcagcag cttttttagg tgggcttcca aaggttatat
     9061 ttgtatattt atcatttttc ttggaagctg cattggcatt tttaattcca tcatcttcag
     9121 gacttgcttc acttacagta cccgtacttg ctcctcttgg tgatttggtt aatatatcag
     9181 cacaagtgat agtaactgct tatcaatttg gtgtaggtct tacaaacttt ataactccaa
     9241 cttcaggagt tttgatggga gcacttgcag tagcccatat accattttca aaatgggtaa
     9301 gattcataat gcctattctg ttgattttaa cagctgtatc tctagtcttt ttaacaatag
     9361 gcatttatat agggttttaa ttaagtatat ttttatttaa gatatttagt aatgatataa
     9421 aatgataata tgttttcaat aaattaccac taatttaatt agtggtaatt tgttttttgt
     9481 atttagaaat aaatgtattt acttgtgttt tgaattatta tctacttaga catagtttta
     9541 attgttatat ttttatttga ttaagcattg atttgttatt ttttatattt catataatga
     9601 catgaaatat aatttttata caataaatga taaaaattta taataaatgt atattttctt
     9661 actcatttct taaaattact catttaattt aataactctc atttcatatg taaaatttgg
     9721 aaaaaattaa aattaaaagt aaaaaactaa aaaaattaat attaatttga aatatatatg
     9781 aaggtttttg gagaatactt acttatgata ggaaaaaatg ttaaaataat agtgtaatca
     9841 aatataaagg actgaaataa ttaacagtta ttaaatatta ggagtgtaaa aatttatggg
     9901 attaaaagaa tttgatttta ttgaagagtc tatcgatatg ttgaggacaa tgtcaccaac
     9961 attagaaact atatcagatg aaatagaaga gtattttgaa aatatcttgg atgaaaagaa
    10021 tcaagaatat ataaatgtaa cctctagaat aaaatcagaa tcaagtttaa gagaaaagat
    10081 aattagaaat agatatttaa aaaaatatgg agaagcaagt aatcttattc ataatgtatc
    10141 tgacttaata ggtttaagaa tcgagtgcag atttattgag gatgaaaaca aaatatatag
    10201 acttttgaga aggtatttta ataaaacaga tgacaaaata aactattata acaaagagaa
    10261 taagaatatt aaattaaaac tatctgagag gcaacccaat aagcaaaaaa atggatttga
    10321 aatatataaa atagatggag ttttcgcgta tttagataga gaagtaaaat ttgaacttca
    10381 aattaaatct cttgtaaatg tgttttggag tgaaatagaa cataaaataa tttataaaaa
    10441 caatacatat ttacttgctg acaaatttat aaaggatatg atggattcta taaa
//
LOCUS       DAFNNB010000135        10318 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0138, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000135 DAFNNB010000000
VERSION     DAFNNB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10318
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0138"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            231..1043
                     /locus_tag="L3M60_002843"
     CDS             231..1043
                     /locus_tag="L3M60_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN6227484.1"
                     /translation="MYLERPELYKQSEINFWDDEYISKQLLKAHLDTNFEGASRNFNF
                     IEDSVNWIVTVANPVKYPKLLDLGCGPGLYAEKFAQKGYKVTGIDFSKRSINYAQNRN
                     KETNLNINYLFQSYLNMNYNEEFDLATLIYCDYGALSTENRRLLMEKIYDSLKPGGKL
                     ILDVFTINKYNNFEEIKYWEINEDGGFWSNEKYMCLQDNCKYNDYNTLEQTLVITEKD
                     ENVFYVWNHYFSKESFLFEIENIGFKSVEFFSNVKGEAYSDDSMTMGLVLQK"
     gene            2092..2853
                     /locus_tag="L3M60_002844"
     CDS             2092..2853
                     /locus_tag="L3M60_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896901.1"
                     /note="N-terminal region resembles flavodoxins. C-terminal
                     ferrodoxin region binds two 4Fe-4S clusters; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EFR1 family ferrodoxin"
                     /protein_id="HBN6227485.1"
                     /translation="MIGIYFSGTGNSRYCVEKFLQEYDITANSYSIEDNELLQCINNH
                     ENIIFSYPVQYSNVPKILKDFIINHSTLWSGKRVFIIATMALFSGDGSGVLSRMLKKY
                     GAITVGGLHVRMPDSIGDEKVLKHSLEYNRKLVIKAEKKVTKAAKKLKYGSPPKEGLG
                     FLSHIVGLFGQRLYFINKTNVYTDKIKINAEECIVCGNCISLCPMKNLVIKNDMIISN
                     GQCTMCYRCVNNCAKKAITLLGKKVVEQSNIKKYM"
     gene            2931..3684
                     /locus_tag="L3M60_002845"
                     /pseudo
     CDS             2931..3684
                     /locus_tag="L3M60_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430254.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
     gene            3970..4389
                     /locus_tag="L3M60_002846"
     CDS             3970..4389
                     /locus_tag="L3M60_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjdF family protein"
                     /protein_id="HBN6227486.1"
                     /translation="MDKISGKLTVLFEEPFWIGIFERQDGKKYEACRVVFGAEPKEVE
                     VYEFILERFFSLDFGSIKLEKNVTKDKIGYKRMQRKVKKEQEKGSIGTKAQNALKLQY
                     EERKQDRKNLAKARKEEEKERLFNLKQEKRKAKHKGH"
     gene            complement(4490..4756)
                     /locus_tag="L3M60_002847"
     CDS             complement(4490..4756)
                     /locus_tag="L3M60_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HBN6227487.1"
                     /translation="MKKFILIYFIVINFIAFFSMYIDKERAIKHKWRIKEATLMYIAV
                     IGGSIGSMIGMYSFRHKTKHIKFTFGIPFIIFLQFLLVYFYILK"
     gene            4983..5684
                     /locus_tag="L3M60_002848"
     CDS             4983..5684
                     /locus_tag="L3M60_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227488.1"
                     /translation="MKFVVEKEVFDKLENVCFGVVVAKGIDNTKEIERISNLLDISID
                     RVEDYFKDKKVKESEEIIPYREAFRSLGMNPNKFMSSIEAMTTRVAKNKKLPHINPIV
                     DLGNSISLKYLLPMGAHDIDFRNDDVYVRFSKKGDKFVPFGETDAELMEEGELIYSVG
                     DMVKTRRWIWRQGEEGKITNSSKNIFFPIDGFTDANLDKVMSAREELAKLLKEIFNCE
                     IKVGFVDKDNPEMEI"
     gene            5766..6374
                     /locus_tag="L3M60_002849"
     CDS             5766..6374
                     /locus_tag="L3M60_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBN6227489.1"
                     /translation="MKKTIIKNVNPMIENKFIGLFEIEYKNKLDEDKVWMVASRKSNE
                     QLKNIYLKNEEDSVDAVVLAGLHKSSKKLILIRQFRVPINDYIYELPAGLVDEGESID
                     VSVERELREETGLTLLEIKKNKSIDKVYLSPGMSDESVAFVYCICDGDITDKFLEADE
                     EIEALLISQEEAKEILQSNHKIDTKAFLILQMFVSLGTKLFE"
     gene            6698..7162
                     /locus_tag="L3M60_002850"
     CDS             6698..7162
                     /locus_tag="L3M60_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4624 family lipoprotein"
                     /protein_id="HBN6227490.1"
                     /translation="MKKLVIILISIINVVSLTACKKSNESKYNNLNNKESGQTTIEME
                     LDKNYDTSDSFVNARLFCVSNDIDVLETEISFQMDGDSGIVEIKDNKTDETLWSNTWH
                     GRVDNDTFTISLANIQKEKEYAIWFTGIKINHAIVKVSFESNLVKEKEIPSK"
     gene            7550..8566
                     /gene="gap"
                     /locus_tag="L3M60_002851"
     CDS             7550..8566
                     /gene="gap"
                     /locus_tag="L3M60_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011948756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBN6227491.1"
                     /translation="MKIKVGMNGFGRIGRAVLRIAQEELGDNIEIVAINARATTESLA
                     HLFTYDSCYGTFRGEVEAKDEDTLVVNSKEIKILRYNDPEELPWKELGVDIVIESTGL
                     FTQREKAEKHIKAGAKKVIITAPGKNEDITIVIGVNEEEYDNEKHNIISNASCTTNCL
                     APFAKVLDEKFGIVKGLMTTVHSYTNDQRILDKSHKDLRRARAAAESIIPTTTGAAKA
                     VSRVLPQLEGKLNGFSLRVPTPTVSLVDLVCELKENVTSEQVNSVLKDAAEGELKGVL
                     GYCDKPLVSIDYKGDSRSSIIDALSTMVIEDNMVKVVSWYDNEWGYSSRTVDLVKYVA
                     EKLK"
     gene            8962..10131
                     /locus_tag="L3M60_002852"
     CDS             8962..10131
                     /locus_tag="L3M60_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227492.1"
                     /translation="MEKLTDRRQVKAVSMETFIFLILLVVGFGYVGSIMGAGMMFKVI
                     MSTAHALLLETVFLIMAMAVLAGALSALLSEFGVIALINKIFAVFMKPLYGLPGASIA
                     GAITTYLSDNPAIIPFAKDKTFTQYFKQYQVPALCNLGTAFGMGLILTTFMISQGTEY
                     VLPALIGNLGAIIGSVISVRIMLTFTKKFYKYNPEEDKATGTLEKKDEFREIREGNVF
                     QRALDAILEGGKMGVDMGMAIIPGVLVVCTLVMLLTFGPSTDPVTGKEVYTGAAYEGI
                     KLLPVIGDKLGFILEPLFGFTSPEAIAFPITSLGAVGAAMSLVPEFIKSGAITPNDIA
                     VFTAMGMCWSGYLSTHIGMMDALNARQLAGKAILSHTIGGLCAGAAAHFIFTLIV"
BASE COUNT         4172 a         1014 c         1782 g         3350 t
ORIGIN      
        1 tattataaaa tcattgatat aagtattgct ggaaaagagt gaataaaatc aaatttaata
       61 agcaaacaaa gtaaagctat agttttattg ttgtgcttaa aaatatttga ttctatttta
      121 ctcttattta aactcatctt tttgaagtta aatcacagag taaactctgt gattttttta
      181 ttttaaaaat aaattttaag gagtggtatg ttttgttaaa caaattatgt atgtatttag
      241 aaagaccaga gctttataaa caaagtgaaa ttaatttttg ggatgatgag tatatttcaa
      301 agcaattatt aaaagcacac ttagacacta attttgaagg agcaagtaga aattttaact
      361 ttatcgagga ttctgtaaat tggatagtta cagttgcaaa tccagttaag tatcctaaat
      421 tacttgattt aggttgtggc ccaggtttat atgcagagaa atttgcacaa aaaggttata
      481 aggtaactgg aattgatttc tcaaaacgtt ctattaacta tgcacaaaat agaaataagg
      541 agactaattt aaacataaat tacttgtttc aaagttattt aaatatgaat tataatgagg
      601 aatttgattt ggctacttta atttattgtg attatggagc tttgtcaact gaaaatagaa
      661 ggttgttaat ggaaaaaatt tatgatagtt taaaacctgg aggaaaatta atattagatg
      721 tatttacaat aaataaatac aataattttg aggaaattaa atattgggaa ataaatgaag
      781 atggaggatt ttggagtaat gaaaaatata tgtgtctaca agataattgt aaatacaatg
      841 attataatac attagaacaa actcttgtaa taacagaaaa agatgaaaat gtgttttatg
      901 tttggaatca ctatttttca aaagaaagtt tcttattcga aattgaaaat attggattta
      961 agtctgttga gttttttagt aatgttaaag gtgaagcata ttctgacgat agtatgacta
     1021 tgggattagt tttacaaaaa taataatttt tttaaaatga ataaataaaa acttgaaacc
     1081 tctaataata aaataaaata agaaaagctt acatattgaa aaatggaaac ttttatgtta
     1141 tttttaatac attacaaatt tgcttatata aaaatcaaca cttaaattat tatttttata
     1201 tgaaatttat ctatagtata tttttataaa caagtaatgc tattaaaatt aagtatatag
     1261 tcaaaagtgt atagcaaaaa ggctgttata ttaatgttag aggttcaggg aaaatatata
     1321 aagcaaagat atttacagat aatgcaaatg gtacaactat aagttagata atagaattat
     1381 gtaatcaaga atttacaaca aagtctaaga taaggataat accaaattgc catacaggtg
     1441 ctagatgtgt aaatgaatta aattaatact attgataaag tacaacaagc acataaacta
     1501 attgatgaga ttattaaaac tataagagat atagttgaca taattaaaat attgtaatct
     1561 atttataatt ttattataaa tcaataatga ttaagattga aataatatta aggcatcaat
     1621 gttataagtt tacaatataa ggagtactat atagtattcc ttacttacac atattaggca
     1681 ataggaagtt ttttcatagt taagttttca aatatttttt agggaacttt gtaattgaga
     1741 attaagggta tagtatgtga tgaacttact aaatttataa ttaggttata taatggaata
     1801 atcatcaata gttttacttt agggaaaata catgcttaag gaagtatagc ttactaatat
     1861 tattaaaatg tatatttaaa atgataaaaa aagaattaga caagtagtta gtaaattgat
     1921 tatgcaaaat tctatcaagc aggaacccta taactttagt catggaaggt tcagtaggta
     1981 atctgttgaa ttaagtttat attatttaaa taaactactt aatttagata atacaaaaag
     2041 tcagaatttt aagaatagag ctatataatc ttagctatta ggagaattac tatgatagga
     2101 atatatttta gtggaacagg aaattcaaga tattgtgtag aaaaattttt acaagaatat
     2161 gatataacag caaattctta ttctattgaa gataatgaac ttttacagtg tattaataat
     2221 catgagaata ttatttttag ttatcctgta caatatagca atgtaccaaa aatattgaaa
     2281 gatttcataa taaatcattc aactttgtgg agtggtaaac gtgtttttat tattgcaact
     2341 atggctctat ttagtgggga tggctctggt gtgttatcta gaatgttaaa aaaatatggt
     2401 gctataactg ttggtggatt acatgtgagg atgccagata gtataggaga tgaaaaggta
     2461 ttaaaacatt ctttagagta taatagaaag cttgtaataa aagcagaaaa gaaagtaact
     2521 aaggctgcta aaaaattgaa atatggatca ccaccaaaag aagggttagg ttttttgagc
     2581 catattgtag gattatttgg acaacgattg tattttatca ataaaacaaa cgtgtatacg
     2641 gacaagataa aaattaatgc tgaggaatgt attgtatgtg gtaactgtat tagtttatgt
     2701 ccaatgaaga atctagtaat taaaaatgat atgattattt caaatggtca atgtacaatg
     2761 tgctatcgtt gtgtaaataa ttgtgctaaa aaagcaatta cattgttagg taaaaaggtt
     2821 gttgaacaga gtaatattaa aaaatacatg taagaacttt aaaattttac taaatataca
     2881 ttgatgtata ctttatgtta gaaagagagc tcaaataatg gaatactacc ataattgatt
     2941 catttgcaac taaattattt aaaggaaata ctgctggaat ttgtgttctt ggcagaagaa
     3001 tacctttaga gttaatacag aaaatagcag aagaaaacaa tttaccagaa acggcatttg
     3061 tagttaaaga taaaaggaat tatgaattat gatggtttac tcctaaagta gagattgatt
     3121 tatgtggtca tgcaatttta gcagctgctt atgtaatatc aaattttatt gatgttaatg
     3181 taaaaaagat agatttcctt actcaaagtg gaaagcttga aattacacga aatgatattt
     3241 ccagaaataa tgtttatcgg aatagagtta tcaccacaac aaactaattt gataggctgt
     3301 gtaccaattg ctgtttattc ttcaagagat ttaattttat tgctaaacag tgaacaagaa
     3361 gttataaatt ataaacctaa ttacacacag ctgagtaaat tgacagactg gttaggtata
     3421 ataatcacag ctcaaggaag taatacagat tttgtatcaa gatatttttg tccagaatta
     3481 gattcaggaa accctgttac tggttcattg cattgtaatc ttattccata ctggtctgaa
     3541 aaggtggaaa aacgcaaaat ggtagctgct caactttcta atcgtggtgg tataattcag
     3601 tgtaaattat taagggataa tactgtaaaa atttctggta aagtagtctt gtttattcag
     3661 gtaacaataa agattgacat ttaaagaagt ataataacta atttataatt taatttacta
     3721 tgaattatga aaaataatta attttcttag agttaattat aataaagtag agtgaaaaaa
     3781 tttaatttca tttaaagcaa tatgatagac gttgatacta agattgacaa gcatgattaa
     3841 aaaagatagt atttaatcta taaagataaa aacacagtct ggttggtagt ccagacgcag
     3901 caatagctgt aagtaacctg cctccttggt tgtccattct ttagaaagta atttaaggag
     3961 gaactagcaa tggacaaaat tagtggaaaa ttaacagtct tatttgaaga accattttgg
     4021 ataggcatat ttgaaagaca agatggtaaa aaatatgaag catgtagggt agtttttggg
     4081 gcagagccta aagaggtaga agtatatgaa tttatacttg aaagattttt ctcattagat
     4141 tttggcagta ttaagttaga aaaaaatgtg actaaagaca aaattggata taaaagaatg
     4201 cagagaaaag taaaaaagga acaagaaaaa ggatctatag ggactaaggc tcaaaatgct
     4261 cttaagcttc aatatgaaga aagaaaacag gatagaaaaa acctagctaa agctagaaaa
     4321 gaagaagaaa aagaaagact ttttaatttg aaacaagaaa agagaaaagc taaacataaa
     4381 ggtcattaat tttaattggc tttcttttta ataattgctt gaaaaggtgt atagaatcaa
     4441 aaactttata caccttaatt tattgaaaaa ataaagcttt atatttcaat tactttaaaa
     4501 tataaaaata tactaataaa aattgtaaaa atattataaa tggtatacca aatgtaaatt
     4561 taatatgttt agtcttatgt ctgaaagaat acattccaat catagaacct atactgcctc
     4621 caattactgc tatatacata agagtagctt cttttattct ccatttgtgt ttaattgctc
     4681 tttccttatc tatgtacatt gaaaagaaag caataaaatt aataactata aaatatatta
     4741 aaataaattt tttcatagtc ttgcctccat aaaaatatat tatacattat aaaataagat
     4801 atttgttttg gaaagttaat tttgcaatta gtacatataa aatacttgat aagaattaat
     4861 ataatattaa ttggaaaatt taaaaaaatt aaacaattag caaatagggg gtatatttat
     4921 aagagacaaa gtgataaaat ttacttagaa aataatgtaa aataacagga ggataagaca
     4981 aaatgaaatt cgtagtggaa aaagaagtct ttgataaatt agaaaatgta tgctttggtg
     5041 tagtagtagc aaaaggcata gataacacta aagaaatcga aagaataagc aatcttttag
     5101 atataagtat agatagagta gaagattact tcaaagataa aaaagtaaag gaatcagaag
     5161 aaattatacc atatagagag gcatttagaa gtctaggtat gaacccaaat aaatttatga
     5221 gttcaataga agcaatgaca acaagagttg ctaagaataa gaaacttcct catataaatc
     5281 ctatcgtaga tttaggaaac tctatttctt taaaatattt acttccaatg ggtgctcatg
     5341 atatagattt tagaaatgat gatgtatatg ttagattttc taaaaaaggg gataaatttg
     5401 taccatttgg agaaactgat gctgagttaa tggaagaagg agaattaatt tattcagttg
     5461 gagacatggt aaaaactaga agatggatat ggagacaagg agaggaaggg aaaattacta
     5521 atagctcaaa aaatatattt tttcctatag atggttttac tgatgctaat ttagataagg
     5581 taatgagtgc aagagaagaa ttagcaaaat tgttaaagga aatatttaat tgtgaaatta
     5641 aagtaggttt tgtagataaa gataatcctg aaatggaaat ataataagtt gttgaatatt
     5701 tgttaagata ttatatttac ttaatgatgt taaaaaggta ttaaaaaaga aaggtcgtag
     5761 taaatttgaa aaaaactata ataaagaatg taaatccaat gattgaaaat aagtttatag
     5821 gactttttga aatagagtat aaaaacaaac tagatgaaga taaggtttgg atggtagcat
     5881 caagaaaaag taacgagcaa ttaaaaaata tttatttgaa gaatgaagaa gatagtgtag
     5941 atgcagttgt attagctggg cttcataaaa gtagtaaaaa attaatttta ataagacaat
     6001 ttagagtacc tattaatgat tatatatacg aattaccagc aggtcttgtt gatgaaggag
     6061 agtctataga tgtttctgtt gaaagagaac ttagagaaga aacagggctt actcttttag
     6121 aaattaaaaa aaataaaagt attgataaag tgtatctatc tcctggaatg agtgatgaat
     6181 ctgttgcctt tgtttattgt atatgtgatg gggatataac agataaattt ttagaagcag
     6241 atgaagagat tgaggctctt ttaatttctc aagaagaggc aaaagagatt ttacaaagta
     6301 atcataagat agacacaaaa gcatttttga ttttgcaaat gtttgtgagc ttaggaacta
     6361 aattatttga atagatatat ggaaaagagg ttatatagag aagtaaaata ataaagttta
     6421 ttttactttt ctaaattata atctcttttt ttatacaata aagtataatt ttgtgtattt
     6481 ttaaataagt gacttgactc ttaaattttc ataatatgtt ataaaccaat tttagatact
     6541 gcaataatta aaatccaaaa attatttaac atatttgcaa ttaaaatgag aaaaggtaaa
     6601 gactgaatga ttgaaaaatt tgatgtgaaa tagctgatta gtttaatatt atagtttgta
     6661 gggttaaaaa ttagaaattg taaaggagat aaattctatg aaaaaattag ttattatttt
     6721 aattagtatc attaatgtgg ttagcttgac agcttgtaaa aaaagcaatg aaagtaaata
     6781 taataatcta aacaacaaag aatcaggaca aacaaccatt gaaatggaat tagataaaaa
     6841 ttacgatact tcagactcat ttgttaatgc acgtttgttt tgtgtgtcta atgatataga
     6901 tgttttagaa acagaaattt cttttcaaat ggatggagat agtggcattg tagaaataaa
     6961 agataataaa acagatgaaa ctttatggag taatacatgg catggaagag ttgataatga
     7021 tacattcaca atttctcttg ctaatataca aaaggagaaa gagtatgcta tatggtttac
     7081 tggaattaaa ataaatcatg caattgtaaa ggttagtttt gaaagcaatt tggttaagga
     7141 gaaagaaata ccctcaaaat aaattccagt ttgtttataa cttaatattc agtacataaa
     7201 agcagttaat atttatgatt gactgctttt tcttatttaa aaaggatgag ctgtgcattt
     7261 ttaattgaaa tagttcatac acattaaaaa ttggtgaata tattactgga gaaggatggc
     7321 aaaatatgtc ccaagggaca ttgagcaaaa taaatgactt cagcttctct gtgctaaaaa
     7381 gtatatacta aataagggtt aaagtagata aaaatgatac actaattgtc ttttgataat
     7441 aaaaaaacat gaaatttaat tatttaatca agaaaaagta ttgaataaat gatttattta
     7501 gtgtacaata atcatgtaaa aacatagtaa tggagggaga catttcataa tgaaaattaa
     7561 ggtaggaatg aacggatttg gaagaatagg aagagctgta ttaagaatag ctcaagaaga
     7621 attaggagat aatatagaaa ttgtagccat aaatgcaaga gcaacaacag agtctttggc
     7681 acatctattt acatatgatt catgctatgg aacttttaga ggtgaagtag aagcaaagga
     7741 tgaagacaca ctagtagtaa actctaaaga aataaaaata ttaagatata atgacccaga
     7801 agaattacca tggaaagaat tgggagttga tatagtaata gaatcaacag gattgtttac
     7861 acaaagagaa aaggcagaaa agcatattaa agcaggagca aaaaaagtaa ttataacagc
     7921 cccaggaaaa aatgaagata taactatagt tatcggagtg aatgaggaag aatatgataa
     7981 tgaaaagcac aatataattt caaatgcatc ttgtactaca aattgtctag ctccttttgc
     8041 taaagtattg gacgaaaaat ttggtatagt aaaagggtta atgactacag tacattcata
     8101 tactaacgat caaagaatat tagataaaag ccataaagat ttgagaagag caagagcggc
     8161 ggctgaatct ataataccta ccactacagg agcagcaaaa gctgtgtcaa gagtattacc
     8221 acaattagaa ggaaaattaa atggtttttc acttagagtg ccaacaccaa cagtttcact
     8281 tgttgattta gtatgcgaac ttaaagaaaa cgttacatct gaacaagtta actctgtgtt
     8341 aaaagatgct gctgaaggag aattaaaagg tgttttagga tattgtgaca agccacttgt
     8401 ttctatagat tataaaggag attcaagatc atctattata gacgctctat ccacaatggt
     8461 tatagaagac aatatggtta aggtagtttc ttggtatgat aatgaatggg gatattcttc
     8521 aagaactgtt gatttagtaa aatatgtagc tgaaaaatta aaatagtata taattcttag
     8581 aataaattaa aaaaaatttt ttgaaaaaaa ttgtataaaa ctaaaaaaaa gattatgaaa
     8641 aatattgaag gaaaaatttt ttcataatct tttttctttc atgcaaaaga tacaagttac
     8701 aacttatatt tgtatattag gtcgttagag aagtatatat atatttagta aggaatcgta
     8761 ctttttataa aatgaaagat aagatgtcga aaacttgcat aaatctgaaa aatttttatt
     8821 attaaaatac tgattttcat tttaaatgtg ataaaaaaat gacaaataaa gttgcaaata
     8881 gtaatactta taagtataat taaaaaagag aaaaggtttt gttaaacttt ataacaaaag
     8941 aaaaagaagg gatgattcaa aatggagaag ttaacagaca ggagacaggt taaggcagta
     9001 agcatggaaa ccttcatttt cctaatatta ctagtagttg gttttgggta tgttggtagt
     9061 attatgggtg caggaatgat gttcaaagta ataatgagta cggcacatgc tttactatta
     9121 gagacagttt tcttaattat ggctatggcg gttcttgcag gcgcattaag cgcactactt
     9181 tcagagtttg gagtaatagc gttaattaat aaaattttcg ctgtatttat gaagccttta
     9241 tatgggctac ctggtgctag tatagcagga gctataacaa cttatttatc agataatcca
     9301 gctattatac cttttgctaa agataaaaca ttcacacagt attttaaaca ataccaagtt
     9361 ccagcattat gtaacttagg aacagccttt ggtatgggac ttatcttaac aacatttatg
     9421 atttctcaag gtacagaata tgtacttcca gcattaattg gtaatttagg tgcaattata
     9481 ggtagtgtca taagtgttag aataatgttg acatttacta agaagtttta taaatacaat
     9541 ccggaagaag ataaagcaac aggaacatta gagaaaaagg atgaatttag agaaatcaga
     9601 gaaggtaatg tatttcaaag agctttagat gcaattcttg aaggtggtaa aatgggagta
     9661 gatatgggaa tggctataat cccaggagtc ctagtagtat gtacattggt aatgttatta
     9721 acttttggac catctacaga ccctgttaca ggtaaagaag tttatacagg agctgcatat
     9781 gaaggaataa aattacttcc agttattgga gacaaacttg gatttatatt agaaccatta
     9841 tttggattta catcaccaga ggcaatagca ttccctataa cttcattggg agctgttggt
     9901 gcagcaatgt ctctagttcc agagtttata aagtcaggag ctataactcc taatgatata
     9961 gcagtattta cagctatggg tatgtgttgg agtggatatt taagtacaca cattggtatg
    10021 atggatgcat taaatgctag acagttggca ggtaaagcta ttctttctca tacaataggt
    10081 ggtttatgtg caggagcagc agctcacttt atatttacac tcatagtata aatgtaatca
    10141 gctaaaataa aaaataatat acaaaaaaat ggttgttgat atgtaataag aatttgcata
    10201 tcaacaacca tttttttctg gctaaagata atttataata cgtaaacatt aagtggatat
    10261 tagagttaag ttatatttat gtaaaaagta acaaaaatct gtagaaattt atgacaaa
//
LOCUS       DAFNNB010000136        10302 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0139, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000136 DAFNNB010000000
VERSION     DAFNNB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10302
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0139"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1307
                     /locus_tag="L3M60_002853"
     CDS             <1..1307
                     /locus_tag="L3M60_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HBN6227493.1"
                     /translation="FIVRQFEKKKIKFSLRTIYATVLGLILGIIIQAFAGFPNDTTQV
                     TWLQEVSKWYSLFGSGFMDLLKMLVVPLVFLSIIKVIINMKENNLGTLTFKSLGMLLA
                     TTAIAAIVGIVVANIMKLGVGANMPTLVENQELKEINSLVDTLRGLLPSNPVMAMANG
                     NIVATIIFATFIATSIKRLSKKHFETIKPFIDFIEASYKIIVSVSMTVIKFMPYAVVA
                     LLANTITSQGISSIVSVLQFILALYISVAILFIIHLVFITLNGLNPITYIKNSTEALL
                     LAFTSRSSLGTLPVTIESLVQNHGVDEGVSSFTASLGANMGMNGCAGIYPALMAVTLA
                     NMAGVNMDMSFYVMLLIVITISSFGIAGLPGTATMAVSVVISGVGLGSYFPLVGGILA
                     IDPILDMGRTMINVNGAMVSSITVGKSLGKINKEVFSKKNIS"
     gene            complement(1388..2596)
                     /locus_tag="L3M60_002854"
     CDS             complement(1388..2596)
                     /locus_tag="L3M60_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227494.1"
                     /translation="MNRFFKVRLITAIMFLIILFAFSIFNICYSGKDMIQEIKNSVAE
                     KSSLKDTISSIDNKVNDKVIYKNTFIETYGYLQKLMGKNEFSKFSVVKDTSGKMHYTY
                     FAKAPNPVGIIADRVRKFSDEMEKQNTKLVYLMTPDKYIKGVTKFPKGIPYSYSNEAA
                     DNFLNQLKKYDVDYIDFRENILKSGIPKDDLFFKTDHHWKVETSFWAFGELVEQLNKK
                     YNMNLDENHYYRDKENYNSIVYPKSFLGSMGRKTGILYGGIDDFTLIYPKFKTNYTYY
                     TDSKSQKFELKGRFEESLILSYPFNADLNLMDGQSDKYFTYLLGNRPLVKIKNIENPE
                     GLKVLFVKDSLIVPTAAFFSSVCSNIDMIDPRYYDGDILEYAKSHDYDFVFISVYPQN
                     LTKEFFPFCE"
     gene            complement(2612..4030)
                     /locus_tag="L3M60_002855"
     CDS             complement(2612..4030)
                     /locus_tag="L3M60_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HBN6227495.1"
                     /translation="MLFSSLIFLFYFLPITLVLYYTFRFNRTIQNMILLAVSLFFYAW
                     GEPKFVVIMITSIIMNYIFGLLVDKYRESKIKVRIFLVLMCAYNIGVLFIFKYLAFAL
                     RNISELINTQLTIPNIVLPIGISFFTFQGMSYVIDVYRRHGEVQKNPFYVGLYIAFFP
                     QLIAGPIVRYESVAEQILNRKETWNKFSIGTCRFITGLGKKVLISNNMAIVADYIYTM
                     NSQGEIAASLAWLGSIAYTLQIFFDFSAYSDMAIGLGLMFGFKFEENFNYPYISKSIS
                     EFWRRWHISLGMWFKSYIYFPLGGSRVANKDIMIRNMFIVWLFTGIWHGAEWTFVIWG
                     ILNFVFLIIERFIMFEKIENHNFIKHIYALLVVNFGWVLFRAPNLKEAYNYFKAMFGG
                     NGVIWSDYTYMFLKEYWIFFIFAFIFSVPIAKKINKFVVEQAKYSMIFNVFYPISAIL
                     LFFISVTYLVTGSYNPFIYFNF"
     gene            complement(4048..5163)
                     /locus_tag="L3M60_002856"
     CDS             complement(4048..5163)
                     /locus_tag="L3M60_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227496.1"
                     /translation="MSLNPWNIFRRDKMKTLKVFFCSAKYFILVFIFFINASFISLAT
                     GDKILDLSFKKIETDLSSKITYEDTGVKIEIDSSKRDKERYLYIYQNIKENWSMYHNF
                     CIEIQNKNKSSQRINLSIQSKNMLEFRLKEGSEIFLEDRNIIYSDKINDGCIEVPGEF
                     EGKIYVNFNNLINEKSNVVLDSNTLSNIVSWGITFIPSDEEHNIVTIKKLSLLSQEKL
                     RFLNNIKIIGDEEVQIPILGQSISQYEVLGLGSNSKIKYSLIGKQNNVNLSQNGKLTL
                     SNKAKPGQIILQVNVDDNFKFGKKITLTESWSINKKDKDGVPYTLVSPEQSPTVQDMK
                     EINFMNSIITFIRILFVSLVVVCFGIYFYWRKCSKTK"
     gene            complement(5135..7441)
                     /locus_tag="L3M60_002857"
     CDS             complement(5135..7441)
                     /locus_tag="L3M60_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBN6227497.1"
                     /translation="MKKNSKKILITLTIITNIVYILWRIFYTVPKEEGMFALICAIIL
                     LFVEIMGMMEMFVHYYGMSNIEYPEKPIISEELYPHVDVFIATYNESVDLVRKTVNGC
                     IHMQYPDRKKVHIYICDDGNREEMHVLAEKMGVNYITRTEREGAKAGNLNNAMQHTNS
                     PLIATFDADMIPMHDFLIATVPYFLKNEQAKKDGEKEEYEKVGFVQTPQSFYNPDLFQ
                     FNLHSEGRIPNEQDYFYRDIQLARNRTNSVIYGGSNTVISREALEEVDGFYTYSITED
                     FATGILIQSKGYRCYAIPDVHASGLSPTDLKSLIKQRERWARGCIQTGRRLNILFRRG
                     LGFWQKISYISSITYWYASIKRFVYIMAPILFSVFNVIVVKCTLLQVLIFWLPMYILS
                     SLSLKIFSQNIRNTRWTNIYETIMFQSLMPAVILETFAISKNKFSVTNKNKLEENRMY
                     KFLQGIPYFIYMVLSIIGILKMFVAIFKMSSMTYSVVLFWLIGNLFNLVMATLFISGR
                     QQLRKSERYTAEIDFKLKQNSYVLSSKTIDISENGFAFLLENPEYISPEEEFEIEFRE
                     KSGNEMYIANMKAKIVNVVELNSKWKYAAYITHIEDSEIDNWMCIVHDRIPTLPMTIS
                     NQLGFFDDLQINVKKRIEKTRTLSRRSPRVNMNFQMDIKNIGKLRIVNFNYQYVLLTF
                     ENKNIYPKEIALEINEGIVLECDLCEGKIDERGILYKVNNIDSIMQNFFLRDEMMDWI
                     LQNKTIIVSKPGEKKEKSIDEFEPMEYI"
     gene            complement(7446..8432)
                     /locus_tag="L3M60_002858"
     CDS             complement(7446..8432)
                     /locus_tag="L3M60_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 5 protein"
                     /protein_id="HBN6227498.1"
                     /translation="MMMFFLGIVLIVFYFVIRGNSDTVKMQRGINIGNALESPKDFHW
                     DVKMSNKFFDDIKDADFDTVRIPIRFSDYTSDSDDFKIDEEFFKKIDGYINYALDKDL
                     IVVLDLHHFEEIMKEPGAHKEEFLKIWEQISNRYQKYDKKLVFELLNEPKENLSSQLL
                     NEYIEEAIKIIRKTNSKRTIIVGPYNFYQIDYLNELNIPKDSNIVVSFHYYEPNDFAF
                     QGNIYHKGFEHLRNITWEGTNEQMNYLKKRFDIVENWANKNKVKIFLGEFGITKEAPE
                     ASRRAWIKAVREEAEKRNFSWAYWELASGFGIYNQIEGNWDRDILSALIEKR"
     gene            complement(8727..>10302)
                     /locus_tag="L3M60_002859"
     CDS             complement(8727..>10302)
                     /locus_tag="L3M60_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4091 domain-containing protein"
                     /protein_id="HBN6227499.1"
                     /translation="SSTISWKGLGNRVRLALNVPNSLENNFSINILGYVQDDTKAFVN
                     DAILRDKDVLVEQYLPQTFWIEGQVPEDFKENNLNIGIDIFKSFGYEDEEKFCTIDVP
                     VKVKNVVLKPLNESKFFLDLWQHPSCLARMYKVELWSDIHFEIIDNYLKELASLGEKV
                     ATVIVSDYPWAGQSCYKVYKNPSNLYEYNMVSVSKGLDGKIKCNFESMDKYISIADKH
                     KMAQEIDLFGLLGNWCAGEFGNPVEGYKDPIRVRYFDESDKVFKFINNTDDLKEYIGL
                     VLNHLIECGLWDRVRIIADEPNNPEVVKECIEFINSTVGTHQVKYKSATHDQNFLDRA
                     KDEIDDMSINLKLTIQNYKDIENLKKKINNNGGILTWFVCCFPEKPNSFISSPFVENR
                     IIGWYTYYFGLDGFLRWDYNLWTEDPWKDSSYKFPIWKAGDMFFVYPGKDLKPVRSVR
                     VENLRFGIQDFELFTMLEKEKGREYIEVELMQELLNKKENAEIKGFGDIELGYSLDNC
                     GYDKVKKYVLELLERL"
BASE COUNT         3531 a         1751 c         1108 g         3912 t
ORIGIN      
        1 tctttattgt tagacaattt gaaaagaaaa aaatcaaatt ttcattgaga acaatatatg
       61 ctacagtact aggacttata ttgggaatta taatccaagc ttttgctggt tttcctaatg
      121 ataccactca agtcacttgg ctacaagaag taagcaagtg gtatagtcta tttggttctg
      181 gatttatgga cttattaaaa atgttagtag tacctttagt tttcttatca attataaaag
      241 taattataaa tatgaaagag aacaatcttg gaactctgac ttttaaatct ttaggtatgc
      301 ttcttgctac cactgctata gctgcaattg ttggaattgt cgttgcaaat ataatgaagt
      361 taggtgtagg agctaatatg cctactctag ttgaaaatca agaacttaaa gaaataaact
      421 ctcttgtgga tactttaaga ggattactac catctaatcc tgtaatggct atggcaaatg
      481 gaaatattgt agctacaata atatttgcta catttatagc tacctcaata aaaagattga
      541 gcaaaaaaca ttttgaaaca ataaaaccat ttatagattt tatagaagcc tcttacaaga
      601 taattgtaag tgtgtctatg actgttatta aatttatgcc ttatgctgtg gttgctctac
      661 ttgcaaatac aataacttca caaggtatta gctcaatagt atctgtttta cagtttatat
      721 tagctcttta tatatcagtt gcaatattat ttatcataca tttagttttt ataactttaa
      781 atggtctaaa tcctattact tatataaaaa attcaactga agctttactt ctagcattta
      841 cttcacgttc tagtttagga acattacctg taacgataga aagcctcgta caaaaccatg
      901 gggttgatga aggtgtatca agctttacag caagtttagg tgcaaatatg ggtatgaatg
      961 gttgtgcggg tatatatcct gctttaatgg ctgtaacact tgctaatatg gctggtgtaa
     1021 acatggatat gagtttttat gttatgttgc taattgttat aacaattagt tcgtttggta
     1081 tagctggtct tccaggaact gctacaatgg ccgtttcagt tgttatctct ggagttggtc
     1141 ttggttctta ttttccatta gttggtggta tacttgcaat agaccctata ttagatatgg
     1201 gacgtactat gataaatgtt aatggtgcta tggtatcatc aattactgta ggaaaatcac
     1261 ttggaaaaat aaataaagaa gtttttagta agaaaaatat ttcttaaaat aagtatgaaa
     1321 ataaatggct aaccttcaac caattagcca tttatttttt attttgttag aaaatttttt
     1381 taggaattta ctcacaaaat ggaaaaaatt cttttgtaag attttgggga tatacagata
     1441 taaaaacaaa gtcatagtca tgagactttg catattctag tatatctcca tcatagtatc
     1501 tagggtctat catatctata tttgaacaaa cagaggaaaa gaatgctgct gtaggtacaa
     1561 ttaaagaatc tttaacaaat aacactttta atccctctgg attttctata ttttttattt
     1621 taactaatgg tctattcccc aacaaatatg taaaatactt atctgattga ccgtccatta
     1681 aattaaggtc tgcattaaaa ggatacgata atatcagact ctcttcaaat cttcctttta
     1741 gttcaaattt ttgagattta gaatctgtat aataagtata attggtctta aatttaggat
     1801 aaattaatgt aaaatcatct ataccaccat ataaaatacc agtctttctt cccatagaac
     1861 caagaaatga cttgggatat acaattgaat tataattttc tttatctcta taataatgat
     1921 tttcatctag attcatattg tattttttat taagttgctc aactaattct ccaaatgccc
     1981 aaaaagaagt ttctactttc cagtgatggt ctgttttaaa aaataaatca tctttgggaa
     2041 taccactctt taagatattt tctctaaaat caatatagtc cacatcatac ttttttagtt
     2101 gatttaaaaa attatctgct gcctcattgc tataagaata aggtatccct tttggaaatt
     2161 ttgtcactcc ttttatatac ttatctggag tcattaaata aaccaattta gtgttttgtt
     2221 tttccatttc atcagaaaac tttcttactc tatctgctat gatgccaact ggatttggag
     2281 ctttagcaaa atatgtataa tgcatttttc cagaagtatc ttttacaaca ctaaattttg
     2341 aaaactcatt tttacccatt aatttttgta aatatccata agtttcaatg aatgtatttt
     2401 tatatattac tttatcattt actttattat caatagatga tattgtatct tttaaactac
     2461 ttttttcagc tactgaattt tttatttcct gtatcatatc ttttccagaa taacaaatat
     2521 taaagataga gaaagcgaat aaaataatta aaaacataat tgcagttatt aatcttactt
     2581 taaaaaatct attcatactt ttccctccca actaaaaatt aaaataaata aatggattat
     2641 agcttccagt tactaaatat gttacagaaa taaagaacaa taaaatagct gatataggat
     2701 aaaatacatt aaatatcata ctatatttag cttgttcaac aacaaactta ttgattttct
     2761 tcgctatagg tacactaaaa ataaaagcaa atataaagaa aatccagtat tcttttaaaa
     2821 acatatatgt ataatcactc catatcactc cattgcctcc aaacatggct ttaaaataat
     2881 tatatgcttc ttttaagtta ggtgctctaa acaataccca tccaaaatta actactaata
     2941 acgcataaat atgttttata aaattatgat tttcaatctt ttcaaacatt ataaatcgct
     3001 ctataattag aaaaacaaag tttaaaatcc cccaaattac aaatgtccat tctgctccat
     3061 gccatattcc agtaaataac catactataa acatatttct aatcataata tctttatttg
     3121 ctactctaga gcctccaagc ggaaagtata tataactctt aaaccacatt cccaaagaga
     3181 tgtgccatct tctccaaaac tcactaattg attttgatat gtatggataa ttaaagttct
     3241 cttcaaattt aaaaccaaac ataagaccta atcctatagc catatctgaa taggcagaaa
     3301 aatcaaagaa tatttgcaga gtataggcta ttgaaccaag ccatgcaagt gatgcagcta
     3361 tttctccttg actattcata gtgtaaatat agtctgctac tatagccatg ttatttgaaa
     3421 ttaacacttt tttacctaga cctgttataa acctacaagt accaatagaa aatttattcc
     3481 aagtttcttt tctattgaga atttgttctg ctacactctc atatctaact ataggacctg
     3541 ctattaattg tgggaaaaat gctatatata atcctacata aaaaggattc ttttggactt
     3601 ctccatgtct acgatataca tctataacat aagacattcc ttgaaatgta aaaaatgaaa
     3661 ttcctatagg taaaacaata ttaggtatag ttaattgagt attaataagt tcactaatat
     3721 ttcttagtgc aaaagccaga tatttaaata taaataaaac acctatattg tatgcacaca
     3781 tcaaaactaa aaaaattctt acttttattt tactttccct atacttatct actaataaac
     3841 caaatatata gttcattatt atagaagtta tcataataac aacaaatttc ggctctcccc
     3901 aagcataaaa aaataaacta acagcaagca atatcatgtt ctgtatcgtt cggttaaatc
     3961 taaatgtata atataaaact aaagttatag gtaaaaaata aaataaaaaa attaaacttg
     4021 aaaataacat aattcaacct cctctcttta cttagttttt gaacattttc tccaatagaa
     4081 atatattcca aaacatacca ctactagaga tacaaataaa attcgaataa atgttatgat
     4141 actattcata aagtttattt ctttcatatc ttgtactgtt ggtgattgtt caggagatac
     4201 cagtgtatat ggaacaccat ctttatcttt tttattaata ctccaagatt cagttaatgt
     4261 tattttcttt ccaaatttaa aattatcatc aacattaact tgtaaaataa tttgtccagg
     4321 tttggcttta ttacttaatg tcaatttgcc gttttgagag agatttacat tattttgttt
     4381 tcctataaga gaatacttaa ttttagaatt acttccaaga cctagtactt catactgact
     4441 tatgctttga cccaatattg gtatttgtac ttcttcatca ccaattattt taatattatt
     4501 taaaaatctt aatttctctt gagacaataa agataacttt tttatagtaa caatattatg
     4561 ttcctcatca gatggaataa atgtaattcc ccaactaaca atatttgaaa gcgtatttga
     4621 gtctagcaca acattacttt tctcatttat aaggttatta aagttcacat aaattttgcc
     4681 ttcaaattca cctggtactt ctatacatcc atcgtttatt ttatctgaat atattatatt
     4741 tctatcttcc aaaaatattt cactaccttc ttttaatcta aattcaagca tattcttact
     4801 ttgtatactt aaatttattc tctgggaact tttattttta ttttgaatct caatacaaaa
     4861 attatggtac atactccaat tttcttttat attttggtaa atatataaat aacgttcctt
     4921 atctctttta gagctgtcta tttctatttt tacacctgta tcttcatatg taattttaga
     4981 acttaagtca gtctctatct tcttaaagga taaatctaaa attttatcac ctgttgctaa
     5041 acttataaaa ctagcattta taaaaaaaat aaacactaaa ataaaatatt ttgcagaaca
     5101 aaaaaacact tttaaagtct tcattttatc tctcctaaat atattccatg ggttcaaact
     5161 catctatact tttttccttc ttttcaccag gttttgaaac gataattgtc ttattttgta
     5221 atatccaatc catcatttca tctcttaaaa agaaattttg cattatgcta tcaatattat
     5281 tcactttata taagatacct ctttcatcaa ttttaccctc acataaatcg cattctaata
     5341 caataccttc attaatttcc agtgctattt ctttaggata aatattttta ttctcaaaag
     5401 ttaataatac atattgataa ttaaagttta ctattcttaa tttacctata ttttttatgt
     5461 ccatctgaaa attcatattg acccttggac ttcttcgtga taatgttcta gttttttcaa
     5521 tacgtttttt tacattaatt tgcaaatcat caaaaaaacc tagttgatta gatattgtca
     5581 ttggtaaagt aggtattcta tcatgaacaa tacacatcca attatctatt tcactatctt
     5641 caatatgtgt aatgtaagct gcatatttcc actttgaatt tagttccaca acattaacaa
     5701 tttttgcttt catattggct atatacattt catttcccga cttttctcta aactctatct
     5761 caaactcttc ttctggagaa atatattctg gattttctag caaaaatgca aacccatttt
     5821 ctgatatatc aatcgttttt gaagaaagaa cataagaatt ttgctttaat ttaaaatcaa
     5881 tttcagcagt ataacgctca gactttctta actgttgtct tcctgatata aatagcgtag
     5941 ccataacaag gttaaaaagg tttccaatca accaaaataa tacaactgaa tatgtcattg
     6001 aactcatttt aaatattgct acaaacattt ttaaaattcc tataatagat aagaccatat
     6061 aaataaaata cggtattcct tgtaaaaact tatacattct attttcttct aacttattct
     6121 tatttgttac tgaaaattta tttttagata ttgcaaatgt ttcaagtatt actgctggca
     6181 ttaaagactg aaacattata gtttcatata tattagtcca tctagtattc cttatatttt
     6241 gagaaaatat tttaagagat aaactactta aaatatacat tggcaaccaa aatatcaata
     6301 cttgaagtaa tgtacatttt actacaatca cattaaatac agaaaacaaa ataggtgcca
     6361 ttatatatac aaagcgtttt atacttgcat accaataagt aattgaagaa atataactta
     6421 ttttctgcca aaatcctaaa cctctcctaa acaaaatgtt taggcgtcta ccagtttgta
     6481 tacatccacg tgcccatctt tcacgttgct taataagact ttttaaatct gtaggagata
     6541 aacctgaggc atgtacatct ggaatagcat aacatctata ccccttactt tgaattaaga
     6601 tacctgttgc aaaatcttct gtaatagaat aagtataaaa tccatcaact tcttcgagtg
     6661 cttcccttga aataactgta ttactaccac cataaattac agaatttgtc ctatttctag
     6721 ccaattgaat gtctctgtaa aaataatctt gctcatttgg aatccttccc tcagagtgca
     6781 gattaaattg gaacaagtct ggattataaa aactctgtgg agtttgcaca aaaccaacct
     6841 tttcatactc ttctttttct ccatcttttt tagcttgctc attttttaaa aaatatggta
     6901 ctgtagcaat taaaaagtca tgcattggta tcatatctgc atcaaatgta gcaatcaaag
     6961 gtgagtttgt atgttgcatt gcattattta aatttccagc ttttgcacct tctctttctg
     7021 ttctagttat ataatttaca cccatttttt ctgctagaac atgcatttcc tcacgatttc
     7081 catcgtcaca tatgtatata tgcacctttt tcctatctgg atactgcata tgaatacatc
     7141 catttacagt ttttctcacc aagtcaacag attcattata tgttgcaatg aatacatcta
     7201 catgtggata aagttcttca cttataattg gtttttcagg atactcaata tttgacatac
     7261 cataataatg cacaaacatt tccatcatac ccattatctc tacaaaaaga agtattatcg
     7321 cacagattaa ggcaaacatc ccttcttctt taggtactgt ataaaaaatt ctccacaata
     7381 tatatacgat attagtaata atagttaaag ttataagtat cttttttgaa tttttcttca
     7441 taaaatcacc tcttttctat taacgcgctt aatatatctc tatcccaatt cccttcaatt
     7501 tgattgtata taccaaatcc agaagcaagc tcccaataag cccatgaaaa gtttcgtttt
     7561 tcagcttctt ctcttacagc ttttatccaa gctcttcttg aagcttctgg tgcttctttt
     7621 gttattccaa attcacccaa aaatattttt accttatttt tatttgccca attttctaca
     7681 atatcaaacc ttttttttaa ataattcatt tgttcatttg ttccttccca agtaatattt
     7741 cttaagtgct caaatccctt atgatagata tttccttgaa atgcaaaatc atttggttca
     7801 taatagtgaa aggaaactac tatattagaa tctttaggaa tatttaattc attcaaataa
     7861 tcaatttgat agaaattata tggtcccact ataatagttc ttttagagtt agtttttcta
     7921 attatcttta ttgcttcttc aatgtattca tttagcaatt gtgaggataa attttcttta
     7981 ggttcattta ataattcaaa taccaatttt ttatcatatt tttgatacct attagatatc
     8041 tgttcccata tttttaaaaa ctcttcttta tgagctcctg gttctttcat aatttcttca
     8101 aaatgatgca aatctaatac aactattaaa tctttatcta atgcataatt tatatatcca
     8161 tcaatctttt taaaaaattc ttcatcaatt ttaaaatcat cagaatctga tgtatagtca
     8221 gaaaacctta ttggtatacg tactgtgtca aaatctgcat cttttatatc gtcaaaaaac
     8281 ttatttgaca tttttacatc ccagtgaaaa tcctttggtg attctagtgc attacctata
     8341 tttatccctc gttgcatctt aactgtatca ctatttcctc ttattacaaa ataaaataca
     8401 ataagaacta tacctaaaaa aaacatcatc ataataccta ttctttttat cttactctcc
     8461 ccctaacact gctatcttat atataatatt attcaatccc tattataaca aattaaatta
     8521 tatcatgtta tatacaatta ttgtaattta caaactttat agtgttgata gttttaaaac
     8581 attaaatgca actaaaaaac aaaaatctat cttgaaatag aaataatcct ttttcattat
     8641 taaagtgatg aaactatatt tgacttcaca caaaaagagt gtctcatgaa ctaaaaagtt
     8701 cattttgaga cactccatta tattttttat aatcgttcta acaactctag cacatatttt
     8761 ttaactttat catatccaca attatcaagt gaatatccca gttctatgtc accaaaacct
     8821 ttaatttcag cattttcttt tttatttaat aattcttgca taagctcaac ttctatatac
     8881 tctcttccct tttctttttc taacattgta aataactcaa agtcctgtat tccaaacctt
     8941 aaattctcca ctctaacact tctaactggc tttaaatctt ttccaggata aacaaagaac
     9001 atatctccag ctttccaaat aggaaatttg tagcttgaat ctttccaagg gtcttctgtc
     9061 cataaattat agtcccatct taaaaaacca tcaagaccaa aatagtaagt ataccatcct
     9121 attattctat tttctacaaa aggagaactt ataaaactat ttggtttctc tggaaaacaa
     9181 catacaaacc aagtcaatat tccaccattg ttattgattt ttttcttaag attttctata
     9241 tctttataat tttggatagt aagttttaaa tttattgaca tatcatcaat ttcatcttta
     9301 gctctatcta aaaaattttg gtcatgggtt gctgatttat atttaacttg atgagttcct
     9361 acagtagaat ttataaattc tatacactct ttgactactt ctggattatt tggttcgtca
     9421 gctataatcc ttactctgtc ccataatcca cactctatta aatgatttaa cactaatcct
     9481 atatattctt ttaaatcatc tgtattattt ataaatttga atactttatc actttcatca
     9541 aaatatctta ctcttatagg gtccttataa ccttctacag gatttccaaa ctcaccagca
     9601 caccaattac ctaatagacc aaataaatct atctcctgag ccattttatg cttatcagct
     9661 atgcttatat acttatccat actctcaaag ttacacttaa tctttccatc caatcctttt
     9721 gatacgctta ccatgttata ttcatataag tttgatggat ttttataaac tttataacaa
     9781 ctttgtcctg cccatggata atctgaaact ataactgttg caactttttc tcctaaagat
     9841 gctaattctt ttaaatagtt atcaattatt tcaaaatgta tatcagacca taactcaact
     9901 ttatacattc tagctaagca acttggatgt tgccataaat ctaaaaagaa cttactttca
     9961 tttaaaggct ttaacacgac attttttact tttacaggaa catcaattgt acaaaatttt
    10021 tcttcatctt catatccaaa gcttttaaat atatcaatac ctatattaag gttattttct
    10081 ttaaaatctt ctggtacttg accttcaatc caaaatgttt gaggcaaata ttgctctact
    10141 aacacatctt tatcccttaa aatagcatca tttacaaatg cttttgtatc atcttgaaca
    10201 taccccaata tatttatact aaaattattt tctaagctat taggtacatt taatgctaat
    10261 cttactctat ttcccagacc cttccatgat atagtactgc tt
//
LOCUS       DAFNNB010000137        10213 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0140, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000137 DAFNNB010000000
VERSION     DAFNNB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0140"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            198..1238
                     /locus_tag="L3M60_002860"
     CDS             198..1238
                     /locus_tag="L3M60_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6227500.1"
                     /translation="MDKHSDSVMYGGMEVYNIWGTTTVDSIIGESDVMRALKNRICKI
                     GNSDSTVAITGESGTGKELIARAIHLAGNRGNKTFVAINCAAIPEPLLESELFGYTKG
                     AFSGADSKGKIGKFELANGGVVFLDEIGDMPIQLQSKLLRVLQEKKFARIGSNELIDI
                     DVRIISATNRDLFELVKENKFREDLYYRLNVIPLEVPPLRERGDDIQILINNFIDKYS
                     RKMKKNVYHIEPDVEQMLLKYPWPGNIRELENTIELALNLLEEDGIIHKGIINRKIIE
                     YFKLNNINIKLLEENDYEISMEQDILTLEEFERAYIKKVLKFYGNDTKSKKLAAKKLG
                     ISLATLYRKIDV"
     gene            1594..2916
                     /locus_tag="L3M60_002861"
     CDS             1594..2916
                     /locus_tag="L3M60_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HBN6227501.1"
                     /translation="MFDIDGRPPILKCLPLSMQHILAMIVGTVTVPIVVGGAVGVSPT
                     QITLLVQYALIIAGISTLIQLYPIGNIGSRLPIIFGVGFTYVPTLVAVGGNYGLASIL
                     GAQLIGGVATVLIGIFIKKIRKFFPNIVAGTVVFTIGLSLYPIAINYMAGGVNSPTYA
                     SMQNWSVAAITLVVVIGLSQFAKGYAKLAAIFLGIIVGYCVSLVLGIINFDPIREATW
                     FAIPKPLEFGMEFDMPVIISVVIVSIINAVQAIGDLSATTMGGMDRNITDKELSSGVI
                     GSGICTMIGALFGGLPPSSYSQNVGMVAMNKVISRFVLGIAGIFMLLSGFVPKFGALM
                     TTIPYSVLGGATISIFSMITMTGIKLIITEELSSRNITIVGLAIALGMGITSVPAAQE
                     AFPSFVKLVFGESPIVIATLVAFVLNLIIPNKSLEDEAKERAVMEETN"
     gene            3213..4574
                     /locus_tag="L3M60_002862"
     CDS             3213..4574
                     /locus_tag="L3M60_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine/pyrimidine permease"
                     /protein_id="HBN6227502.1"
                     /translation="MKTRNTSKYDVDGIPPLKESIPLALQHLLAMIVGNMVPAILIAN
                     VVGLNQGQATMLIQGSMLAAGLATFLQLYPIPLFKGFKLGSRLPVMMGMSYVFLGACL
                     SVAAKDGLAALFGAQIAAGVIVFFVGFGVKKIRHIFTPIVSGTIIACMGLGLFATAIK
                     NLAGGEGAQTFGSPINFTVGVIVAFVIIMINKYGKGLVKNSSILIGILVGYAISLVLG
                     LVDFSAVQGAAIVSLPTPAAFGLEFRPELIVMFTIIYIIGIADMMGACTIATVGAMDR
                     EVTDEELASVVLGNSVTSIISSLFAALPTGVFSQNTVIVSMNKVTSRFVIALGALVLL
                     LAGISPALGAIMTTIPSCVVGGATLVVFSSIAMSGFSIMSMDGFTEENNLIAGVSIAT
                     SMGLTTAPQVLDQFPETIGTVLGGSSIVSGAIIAILLQTLFKLKSRKSAENVTSNLEE
                     NIG"
     gene            4647..6041
                     /locus_tag="L3M60_002863"
     CDS             4647..6041
                     /locus_tag="L3M60_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6227503.1"
                     /translation="MIDILIKNAIIVTVNKDRDVIFDGAIAIQGDRILDIGNTVDIEP
                     KYLDAKRVIDANGKVIFPGFINTHNHLFQVLLKGLGDDMALHEWLNTMMFPSAKFLTE
                     QDTYDAAMLGCMEGLKSGITTMVDYMHTHNRPGLTDGIVKAYKDLGIRGVVGRGCIDL
                     GIHKELIEDVETVEKDLRRVFEKYHNSENGRIKICVAPSSMWAISEEMGKMLWNIVKE
                     YDSYFTVHISETEFARTATKDIHGEIDIKLLEKWGIVGPEVVAVHCVCITDEDMEMLK
                     KYDIKVSHNVASNMYLASGVAPVPEMLKKGINVSLGLDGAASNNAQDMIELMKLTALQ
                     HKVNNCDPLAISAEKVLEMATIEGARTLRMEDEIGSLEIGKKADLVIFNSMLSPKSIP
                     LHNPVSTLVYSASMHNVESVLVDGNIILENGKVTTIESEEQVLLNAQHAAEELCKRAN
                     VTNRMEGHKWNSLY"
     gene            6103..6573
                     /locus_tag="L3M60_002864"
     CDS             6103..6573
                     /locus_tag="L3M60_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBN6227504.1"
                     /translation="MEKIKLNINDYMYEIEVEKNETLLHVLREKLNLTGAKQGCNSGS
                     CGACKVLIDGVDMKSCKLLARNCEGKVIYTIESFANGENLHPIQEAFIEVGAVQCGYC
                     TPGMIITTQALLNRNASPNEDEIREAFKENLCRCTGYVKIIEAVKLSAARLGGR"
     gene            6576..8792
                     /locus_tag="L3M60_002865"
     CDS             6576..8792
                     /locus_tag="L3M60_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBN6227505.1"
                     /translation="MEKVIGKSYPLKDAALKVTGQMKYVADMKPQNVLYAKMLLSPVA
                     HAKIKSIDTSEAEKVVGVRAIATYLNTSQRKYNGALRFYEHNIPENEVIFSDTVRFVG
                     DRVAAVAAETPEIAEKAIKLIKVEYEELPAVFTIQEAIKEDAICIHGDSNIVSQNNIN
                     GGDVEKGFKECDYIFEDEYSTPAIHHSAIETHSVIAHYDYNDKLTVHTPCQNTFGFRI
                     ILSQIFGMSLNKVRVIRPAIGGSFGGKLELTIEPVAAALSKMTRRPVKMVLTRKESMI
                     STRTRHGSFVKLKTGVNKDGEVIAQDIKIYTNTGAYASSALNVIGALSHKVFKVYKIP
                     NIKFTGMPVYTNTPIAGAMRGYGSPQIFMAQQAQFAKIAKELGMDLVDFQNKNAVEPD
                     DVDIIFHGSLGNPRVLDCIEQGKRMFKWDEKKKLPKEEGKYLRGIGMAIGAHGNGVFG
                     AHRDVVTLTLKLNEDGTLTLITGAHDMGNGVVTMQTMMVAEILGITPDKVDTFETDTD
                     ACTFNLGDYASRGVFVEGGGAKKTAEKLKVLILEEAEKLLETSKEDLYLDNGYVISKL
                     NENIKASLSDVAVYSQSKSLRELTVVEDYSSPTGLTSYGVHFAEVLVDKETKDIKVVD
                     FVAVHDVGRVINPLNLEGQLEGGIHMGLGYALSEELAFDEQGRPKATDFKTYKLFRTV
                     DMPKCQVAFVNSYEKAGPFGGKSIGECAVVPVAPAVANAVYNATDVEIHSLPIKLK"
     gene            8872..9543
                     /locus_tag="L3M60_002866"
     CDS             8872..9543
                     /locus_tag="L3M60_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6227506.1"
                     /translation="MAKILGICGSPRKGATEYAVLEALKEAETIPGIETEYWSVRGKK
                     ISPCVHCDACIRKETMCIIKDDIQELEQKILEADGIIVGSPVYDMNITAQLTAVFNRL
                     RPMYLVHPGKLQNKVGAAITTGGTRYGGQELTKLPIINFFLMHEMLVSGGLGGCYIGG
                     TIWSKDGKAKGAQEDETGMDTVKRLGKGAAEAVMVSKFGLEKWNVVKEELDVKKDEKS
                     PLRDH"
     gene            9906..>10213
                     /locus_tag="L3M60_002867"
     CDS             9906..>10213
                     /locus_tag="L3M60_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBN6227507.1"
                     /translation="MLKEIKWKVNNLPKGDKENCIKFLNEEEITKVRNFHKSFPQYKE
                     TPLANLEGLAKKLGVAGVYVKDESYRFGLNAFKVLGGSYSMGKYLAQRLDTDISELGY
                     "
BASE COUNT         3833 a         1180 c         2034 g         3166 t
ORIGIN      
        1 attgcttatt taatagagga ttcttgtaaa gttaagaaag tatatctatc aaattaaaac
       61 taagtggata tactttttat taagttttgt tttaaaataa tattattaaa ttatagctat
      121 gcttttctgt tatgatataa ttaaatagat aatgtgaatg attattatat agaatactta
      181 tttggagttg atattttatg gacaaacaca gtgattcagt aatgtatggt ggaatggaag
      241 tatacaatat ttggggaaca acaacagtag actccataat tggagagtct gatgtaatgc
      301 gtgctcttaa gaatagaatt tgcaaaattg gaaatagtga ttctactgta gctataactg
      361 gagaaagtgg cactgggaaa gaactaatag caagagccat tcatctcgca ggaaacagag
      421 ggaataaaac attcgttgct ataaactgtg ctgctatacc agaaccttta ctagaaagtg
      481 aactttttgg atatacaaaa ggagctttta gtggagcaga ttcaaaaggt aaaattggta
      541 agtttgaatt ggccaatgga ggggttgtat ttttagatga gataggagat atgccaattc
      601 aactacaatc aaagttattg agggtattac aggaaaagaa gtttgctaga ataggttcaa
      661 atgaactaat tgatatagat gttagaatca tatcagctac taatagagat ttatttgagt
      721 tggttaagga aaataaattt agagaagact tatactatag attgaatgta atacctttag
      781 aggtgcctcc acttagagaa agaggagacg atatacaaat attaataaac aattttatag
      841 acaaatattc gagaaagatg aagaaaaacg tgtaccatat agaaccagat gtggaacaaa
      901 tgctgcttaa gtatccttgg ccagggaata ttagagagtt ggagaatact atagaacttg
      961 ccttaaatct tcttgaggaa gatggaataa tacataaagg tataataaac agaaagataa
     1021 ttgaatattt taagctaaat aatataaata taaagctact tgaggaaaat gactatgaga
     1081 taagtatgga acaagacata cttacgcttg aagaatttga aagagcctat ataaagaaag
     1141 tgttaaagtt ttacggaaat gatactaagt ctaaaaaact ggctgctaaa aaacttggaa
     1201 tatcactagc tacattgtac agaaaaatag atgtataata attagacttt ttattctcaa
     1261 ttctaaaatt tgaaattata aaattgataa aaaacacttg gaaaactatt ttctaagtgt
     1321 ttttttgtgc aatagtattt tattatccaa aaataaaaaa aatttgaaag aatttaaaaa
     1381 aagaaatgct ttaataaatt atcaaatact aacaaatata ataaaaatta tatatgtaaa
     1441 acaattatgt taaaaaattg gcattataat tgcttcatat taagttgaga tgacaaaata
     1501 aaaaaagaaa ggatgttgtg tgagataata ataactgtca ttaaaaaata gttgggagtg
     1561 ataaccttga gtaaatatca aaaaaatgtt tctatgtttg atattgatgg aaggccacca
     1621 attttaaagt gtttaccact atcaatgcaa catatattgg caatgatagt tggaactgtt
     1681 acagtaccta tagtcgtagg aggagctgtg ggtgttagcc ctacacaaat aacactttta
     1741 gtgcaatatg cattaataat agctggtata tcaacactta tacaactata tccaatagga
     1801 aatattggtt caagattgcc tataattttt ggagttggtt ttacttatgt accaacactt
     1861 gtagcagtcg gaggaaatta tggattagca agcattttgg gtgcgcaatt aataggggga
     1921 gtggctacag tattaatagg gatatttata aaaaagataa gaaaattttt tccgaatata
     1981 gttgcaggaa cagttgtttt tacaattgga ttatctttgt atcctatagc aataaattat
     2041 atggcaggtg gtgtaaatag tcctacatat gcttctatgc aaaactggtc agtagcggct
     2101 ataacactag tggtagttat aggtcttagt caatttgcaa aagggtatgc aaaattggcg
     2161 gcaatatttt tgggaattat agttggatat tgtgtatctt tagttttggg tataataaat
     2221 tttgacccta ttagagaggc aacttggttt gcgataccta aaccacttga atttgggatg
     2281 gaatttgata tgcctgtaat aatttcagta gtaattgtaa gtataatcaa cgctgttcaa
     2341 gcaattggag atttatcagc tactacaatg ggaggtatgg ataggaatat cacagacaag
     2401 gaattatcaa gtggagtaat aggaagtgga atttgtacta tgataggagc tttgtttgga
     2461 ggtttgccac catcatcata tagccaaaat gtggggatgg tagctatgaa caaagtaatt
     2521 agtagatttg tgcttggaat agctggtata tttatgttat tgtcggggtt tgtgcctaaa
     2581 tttggtgcat taatgactac cataccatat tctgttttgg gaggagctac aatatctata
     2641 tttagtatga taacaatgac aggtataaag ttaattatta cagaagagtt gtcatcaaga
     2701 aatattacca ttgttggttt ggcaattgct ttaggcatgg gaataacatc agtcccagca
     2761 gcccaagaag cgtttccttc atttgtaaag ttagtatttg gagaatctcc aatagtaatt
     2821 gcgacattgg ttgcattcgt tttaaatcta ataataccaa ataaaagtct agaggatgaa
     2881 gctaaagaaa gagctgttat ggaagagact aattaagata taatagtttg aaaggattgt
     2941 tcaaattatt tagtatgaga ttgttagatt atttttgata gctattcatt ttaataaaaa
     3001 atttaacaaa taaatatata ccaaataatt aatatttatt taagttgaaa atcggggagg
     3061 taatatttag aaatttgtta attttttata aaaatagctc agtttagctg tgtttatcgg
     3121 ttgagcaata aattcatggt cataaaaata tttatagaaa ataattataa aaatatttag
     3181 tcaagatatt tatgtatggg aggatttatt ttatgaaaac acgtaatact tcaaaatatg
     3241 atgtcgatgg gataccacca ctaaaggaat caataccttt agccttacaa catctgttag
     3301 caatgatagt aggaaatatg gtacctgcga tattaatagc aaacgtggta ggacttaacc
     3361 aggggcaagc aacaatgttg attcagggtt ctatgttagc agcaggtttg gcaacgtttt
     3421 tacaattata tccaatacct ttatttaaag gctttaaatt aggttcaaga ttacctgtaa
     3481 tgatgggaat gagttatgta tttttaggag cctgtctttc agtagccgct aaagatggtt
     3541 tagctgctct atttggagca cagattgctg ctggggtaat tgtattcttt gtaggttttg
     3601 gagttaagaa aattagacat atattcactc caatagtttc tggaactata atagcatgta
     3661 tgggacttgg attatttgca acagctataa agaatttagc tggaggagaa ggtgcacaaa
     3721 catttggaag cccaataaac tttacagtag gagttatagt tgcttttgta ataataatga
     3781 taaataaata tggtaaaggt ttagtaaaaa attcttcaat attaatagga atattagtag
     3841 gttatgctat atctttagtt ttaggattgg ttgatttctc agccgttcag ggagcagcta
     3901 tagtatcatt accaacacca gcagcttttg gattagaatt tagaccagag ttaatagtaa
     3961 tgtttactat tatttacata ataggtatag ctgatatgat gggtgcctgt acaatagcta
     4021 cagtaggagc aatggataga gaggtaactg atgaagaatt agcatcagtt gttttaggaa
     4081 actctgtaac ttctataatt tcatcactat ttgcagctct tccaactgga gtatttagcc
     4141 aaaatacagt aattgtatct atgaacaaag ttacaagtag atttgtaata gcactagggg
     4201 cacttgtatt attactagcg ggaatatctc cagcattggg cgcaataatg acaactatac
     4261 catcttgtgt tgttggtgga gcaactttag tagttttctc atcaatagca atgtctggtt
     4321 tctctattat gagcatggat ggatttactg aagaaaacaa tcttatagca ggtgtatcta
     4381 tagcaacaag tatgggtctt acgactgctc cacaagtttt agaccaattc ccagaaacaa
     4441 ttggaactgt ccttggaggg tcaagtatag tttctggagc aataatagct atattacttc
     4501 agacactatt taaattaaag tcacgcaaaa gtgctgaaaa cgttacttca aatcttgagg
     4561 aaaatattgg ataaatgtat tagatataat atgagaaaac catatggtaa aatgttgtaa
     4621 cgaaagtatt caaaggggga tttaagatga ttgatatact aataaaaaat gcaataattg
     4681 ttacagttaa taaagacaga gatgttatat ttgatggagc aatagctatt caaggtgaca
     4741 gaatcttgga tataggaaat acagttgaca ttgaacctaa gtatcttgat gctaaaagag
     4801 ttatagatgc taatggaaaa gtaatattcc ctggctttat aaatactcat aatcacttgt
     4861 tccaagtatt attaaaaggt ctaggcgatg atatggcact tcatgagtgg ttaaatacaa
     4921 tgatgtttcc atcagcaaaa ttcttaacag aacaagatac ttatgatgct gcaatgcttg
     4981 gatgtatgga aggtttaaaa agtggtataa ctacaatggt tgattatatg catactcaca
     5041 atagaccagg acttacagat ggaatagtaa aagcatataa agatttaggt ataagagggg
     5101 ttgtaggtag aggttgtata gatttaggta ttcacaaaga gcttatagaa gatgtggaaa
     5161 cggttgaaaa agatttgaga agagtatttg aaaaatatca taattcagaa aatggaagaa
     5221 taaaaatctg tgtagctcct tcttcaatgt gggctatatc tgaagaaatg ggtaagatgc
     5281 tttggaatat agttaaagaa tatgattcat attttacagt tcatatatct gaaacagaat
     5341 ttgcaagaac agcaacaaag gatatacatg gagaaataga tataaaatta cttgaaaaat
     5401 ggggcatagt aggtccagaa gtcgtagcag tacattgtgt gtgcataact gatgaagata
     5461 tggaaatgct taaaaagtat gatataaaag tttctcacaa tgtagcaagt aatatgtatc
     5521 ttgcttctgg tgttgctcct gttccagaaa tgttaaaaaa aggtataaat gtgagtttag
     5581 gtcttgatgg tgcagcaagt aataatgcac aggacatgat agagcttatg aaactaacag
     5641 ctttacaaca caaagtaaat aattgtgacc ctctagcaat ttctgcagag aaagttcttg
     5701 aaatggctac aatcgaggga gcacgaactc ttagaatgga agatgaaata ggctctttag
     5761 agattgggaa aaaagctgac ttagtaatat ttaactcaat gctttctcca aaatcaatac
     5821 ctcttcacaa tcctgtatca actttggtat actcagctag tatgcacaat gtagagagcg
     5881 ttttagttga tggaaatata atacttgaaa atggaaaagt aactacaatt gaaagtgaag
     5941 aacaagtgtt gcttaatgca caacatgcag cagaagaatt atgtaagaga gctaatgtaa
     6001 ctaatagaat ggaaggacat aagtggaata gcttatacta atattaaaat aagattgtaa
     6061 taatattcta ttacaacatt agtttatctg taggaggaga aaatggaaaa gataaaatta
     6121 aatattaatg attacatgta tgaaatagaa gttgaaaaga atgaaactct tttgcatgta
     6181 cttcgtgaaa aattaaatct aactggagcg aaacaaggtt gtaatagtgg cagttgtgga
     6241 gcatgtaaag ttttgataga tggagttgac atgaaatctt gtaaacttct tgcaagaaac
     6301 tgcgaaggta aagttatata cactatagaa agttttgcta atggagaaaa tcttcatcca
     6361 atacaagaag cttttataga agttggagct gtacaatgtg gatattgtac tcctggtatg
     6421 attataacaa cacaagctct tttaaataga aatgcatctc ctaatgaaga tgaaattaga
     6481 gaagccttta aagagaatct ctgtagatgt acaggatatg taaaaattat agaagctgta
     6541 aaattatctg cagctagatt agggggtaga taattatgga aaaagtaata ggaaaatcat
     6601 accctctaaa agatgcagct ttaaaagtta ctggtcaaat gaagtatgtg gctgatatga
     6661 aaccacaaaa tgtactttat gctaaaatgc ttttaagccc tgtagcacat gcaaaaataa
     6721 aaagtataga tacaagtgaa gctgagaaag tagtaggtgt tagagctata gctacttatc
     6781 taaatacatc acaaagaaaa tacaatggtg cattaagatt ttatgagcac aatatacctg
     6841 aaaatgaagt tattttttca gatacagtta gatttgttgg tgaccgcgtt gcagcagtag
     6901 ccgcagagac tccagaaata gcagagaaag ctataaaact tataaaagtt gaatatgaag
     6961 agttaccagc tgtatttact atacaagagg caataaaaga agatgctatc tgcatacatg
     7021 gagattcaaa tatagtttct caaaataaca taaatggggg tgatgtagaa aaaggcttca
     7081 aggaatgtga ctatatattt gaagatgaat actctactcc agcaatacat catagtgcaa
     7141 tagagactca tagtgtaatt gcacactatg attataatga taaattaact gtacatacac
     7201 cttgccaaaa tacatttgga tttagaataa tactgtctca aatttttggg atgtcattaa
     7261 ataaagtaag agttataaga cctgctattg gaggttcatt tggaggaaaa ttagaactta
     7321 caattgagcc agtagcagcc gcattatcaa aaatgacaag aagaccagtt aaaatggttc
     7381 ttacaagaaa ggaatctatg atatcaacaa gaactcgtca tgggtccttt gtaaaattaa
     7441 aaactggtgt aaataaagat ggcgaagtaa tagcacaaga tataaaaata tatacaaata
     7501 caggcgcata tgcatcaagt gctttaaatg tcataggagc acttagtcat aaagtattta
     7561 aagtttataa aataccaaat ataaagttta ctggaatgcc tgtatatact aatacaccaa
     7621 tagcaggagc tatgagaggt tatgggtcac ctcaaatatt tatggctcaa caggctcaat
     7681 ttgcgaaaat agccaaggaa ctaggtatgg atttagtcga ttttcaaaat aaaaatgcag
     7741 tagagccaga tgatgtcgat ataatcttcc acggttctct tggaaatcct agagtattgg
     7801 attgtataga gcaaggtaag aggatgttta agtgggatga aaagaaaaaa cttccaaaag
     7861 aagaaggtaa atatttaaga ggtataggta tggcaatagg tgcacatggt aatggagttt
     7921 ttggtgcaca tagagatgtt gttacattga ctcttaaatt aaatgaagat ggtacattga
     7981 cactaataac aggagcacat gatatgggta atggtgttgt aactatgcaa acaatgatgg
     8041 tagctgaaat tttaggaata acacctgata aagttgatac atttgaaact gatacggatg
     8101 catgtacatt caaccttggt gattatgcta gtagaggggt atttgtagaa ggtggaggag
     8161 ctaaaaagac tgcagaaaaa ttgaaagtcc ttatattaga agaagcagaa aaattacttg
     8221 aaacatcaaa agaagattta tatctagaca atggatacgt aattagtaaa cttaatgaaa
     8281 atataaaagc atcactttct gatgtcgcag tatatagtca aagtaaatca ttaagagagt
     8341 taactgttgt tgaagattat tcttcaccaa ctggacttac ttcttatggt gtacattttg
     8401 ctgaagtttt agtagataaa gaaactaaag atataaaagt agttgatttt gtagctgtac
     8461 atgatgttgg tagagttata aatcctctaa atttagaagg tcaattagaa ggcgggattc
     8521 atatgggtct aggatacgca ttgagtgagg agttggcatt tgatgaacaa ggtagaccta
     8581 aagctactga ttttaaaaca tataaattat ttagaactgt agatatgcca aaatgtcaag
     8641 ttgcatttgt gaatagttat gaaaaagctg ggccttttgg tggtaagagt attggagaat
     8701 gtgctgttgt accagttgca ccagctgttg caaatgcagt atataatgca acggatgtag
     8761 aaatacattc attgccaata aaattaaaat agtagaaaaa gcataaataa caatttattg
     8821 tatttgtaaa tagataataa aaatagattt atttggtgag gagatattat tatggcaaag
     8881 atattaggga tttgtggaag tccacgtaaa ggagcaacag aatatgcagt gctagaagct
     8941 ttgaaagaag cagaaactat accaggtata gaaactgagt attggtcagt tagagggaaa
     9001 aaaataagtc cttgtgtaca ttgtgatgca tgtataagaa aagaaactat gtgtattata
     9061 aaagatgata tccaagaatt agagcaaaaa atattggagg cagatggaat tatagtaggt
     9121 tctccagttt atgatatgaa tataactgct caacttacag cagtgtttaa tagacttcgt
     9181 ccaatgtatt tagttcatcc aggaaaactt caaaataagg ttggagcagc tataactact
     9241 ggtggaacta gatacggtgg tcaagaattg acaaaacttc ctataataaa tttcttccta
     9301 atgcatgaaa tgttagtatc tggaggttta ggtggatgct atataggtgg tacaatatgg
     9361 agtaaagatg gaaaagcaaa aggtgcccaa gaggatgaga caggtatgga cacagtaaaa
     9421 agacttggta aaggtgctgc agaggcagtt atggtttcta agtttggtct tgaaaaatgg
     9481 aatgtcgtaa aagaagaatt agatgttaaa aaagatgaaa aatcacctct tagagaccat
     9541 taaatattta ttatattatt aaatatatac aaatagtgat gtaatagatt attgcatcac
     9601 tattttatgt tttaatttag attttataaa ttttatatta aaaaatatag aaacaaagtg
     9661 ttttgagatt tgtttttaaa ttttaaaaat gagaattaaa aagtgagaag atagaaaagg
     9721 ttttttagtt attaaattct atatacattg tatgaactaa agatagaaaa gaaaaaataa
     9781 taaaagcatt gcaatttcta tactaaagag tcatggaaaa atatttagta tatatttagt
     9841 gttggcatgt taattgctta tatattaaag tataaaaatt taacaataat ttggaggtat
     9901 tttagatgtt aaaagaaatt aaatggaaag taaataattt gccaaagggt gataaggaaa
     9961 attgtatcaa atttttaaat gaagaagaaa taactaaggt tagaaacttt cacaaaagct
    10021 ttcctcaata taaagaaact ccattagcaa acctagaagg tttagcaaaa aaattaggtg
    10081 ttgcaggagt atatgtaaaa gatgaatctt atagatttgg attaaacgca tttaaagtat
    10141 taggtggctc ttattctatg ggtaaatatt tagctcaaag attagataca gatatatctg
    10201 agttagggta tga
//
LOCUS       DAFNNB010000138        10189 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0141, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000138 DAFNNB010000000
VERSION     DAFNNB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0141"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            14..631
                     /locus_tag="L3M60_002868"
     CDS             14..631
                     /locus_tag="L3M60_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBN6227508.1"
                     /translation="MEIFAIGHSNYPYDKLIDMIKKYDINCVVDIRETPYSKYNTQYN
                     KEAFNESLRNSGFVYIYMGKEFGAKRTNKDVYTQEGYADFEKVAKEDIFLKGIDRLKK
                     GCQMGYRIVLLGAMQEPIRCHRSILVGKILNKEGFDVKYIMHEGNLAYQQDIEESLLD
                     KYFADRKQLSIDNLLGSALTREEMIQEGYKLANKEIGYRTEKLGK"
     gene            complement(787..1239)
                     /locus_tag="L3M60_002869"
     CDS             complement(787..1239)
                     /locus_tag="L3M60_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227509.1"
                     /translation="MVNLNENNLNVNMVYPISPTAPIRFRRYTVSYLENMENIYLTIA
                     TYFDTIGLGTMNIDKVYGQWAWFTDTIYDLNLFVFVGNYPYQIAKNRYDTFVNILPVA
                     ITSIVNGDRVFLISNPCLLNSKITVRFISSHPTLNRTVDYGNIRDFIF"
     gene            1523..2881
                     /locus_tag="L3M60_002870"
     CDS             1523..2881
                     /locus_tag="L3M60_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6227510.1"
                     /translation="MFIRKNVIKLAIPIMIEQTFVMLLGVFNTMMAGHIGEEAVSAVG
                     MVDSINNIFISVFAALSVGATVVVAQQIGKKDNEKVNETIKQALVSGAILSFTITILM
                     WIFRTGVINLFYGSAEELVKSNAKLYIEFTLFTYPFIAIQQIANGILRGCGDVKKPMY
                     VTMFMNIVNVTLGYILIYGVDLNPLGINLQTPSYGITGAAMSIAIARIIGCIVIVRAL
                     FKGNEFISIGKIFPFKIDVETQKSIFNIGIPAGVEQLLFNAGKLIVQVFIVTMGTSAI
                     ASNAIGMSIASIINVIGNALALSATTLVGQYIGRGDIKGAKSTLLYLTKFATICLFVV
                     GAIFVPIAPWISSLYTQNASVIDISTQLIRSDCLAMVIWPISFVLSAGLKGAGDTRYT
                     MMTAVIGMWIFRIFTGYVLGVVLEFGVLGIWIGMYTDWLVRGTLYCLRLRGTKWLKHK
                     IA"
     gene            3154..3534
                     /locus_tag="L3M60_002871"
     CDS             3154..3534
                     /locus_tag="L3M60_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN6227511.1"
                     /translation="MINKIGKITLYVNNQEDAKLFWTEKLNFVVTYEKEMGPNMKWVE
                     VAPSKNEFTTFVLYDKNLMMKQNKEINVSHPSIILSTRDINGTYEQMANNEVEVGELM
                     EMPYGRIFSFKDQDGNDYLVREDK"
     gene            3659..3859
                     /locus_tag="L3M60_002872"
     CDS             3659..3859
                     /locus_tag="L3M60_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227512.1"
                     /translation="MFKKMAVLKDIATKIGRKKAYELLEMVEGNDAFVAEVKIKKNGI
                     ESKKEEIMLKDNQKIILEYIEG"
     gene            4055..4783
                     /locus_tag="L3M60_002873"
     CDS             4055..4783
                     /locus_tag="L3M60_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6227513.1"
                     /translation="MLEIKNLSFNVESNSEELGIINDVSLSFEKGKLIVITGPNGGGK
                     STIAKLIMGIEKATSGQIILDGEDITNLSITERAKKGIGYAFQQPPRIKGMTVENLLT
                     LAHGKPLSTDVCCQYLTDVGLCSKDYLNREVDNSLSGGEMKRIEIATLFARDLKVSIF
                     DEPEAGIDLWSFGKLNESFKKIHEESDQTIIIISHQERILELADEIIVLQDGSVKSHG
                     TKEAILPEIVCQVNSSCELMKDMN"
     gene            4805..5722
                     /locus_tag="L3M60_002874"
     CDS             4805..5722
                     /locus_tag="L3M60_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HBN6227514.1"
                     /translation="MNKIDLNLLEKVAELRGTPLGAYNIRKNGQGVERHSTENVTIIP
                     KTDKPGIDIIVKPNTKGENVHIPVIVTDTGVQDMVYNDFEIGENADVVIIAGCGIHNC
                     GCDDSEHNGIHRFFLRKGARLKYVEKHYGEGDGKGKRILNPVTIVNMDEDSYCEMETI
                     QIKGVDSTKREMDANLGKGAKIVVFEKLLTHGNQVAESNMKVVLEGENSSAQVVSRTV
                     GQDNSKQKFNPCVIGDSLCSAHVQCDSIIMGNSQISAIPEIAANHPDALLVHEAAIGK
                     IAGDQILKMMTLGLTEEEAEQQILNCFLK"
     gene            5859..7043
                     /locus_tag="L3M60_002875"
     CDS             5859..7043
                     /locus_tag="L3M60_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBN6227515.1"
                     /translation="MKNLLIRNGTIISPTDGIKFERRDILVLHGKIYAIGLNLKEKIR
                     KEKLLTNKEFEIINADNMYISPGFIDVHTHCYKRKLPTGMDSDSIGIEKGSTVIFDAG
                     TAGPLNYYDFKKKYMDECKTEVYSFLNVTNEGLNILNESTSLDVINFDKVESVVEKNI
                     EKIKGIKVKASKFQSNKNGIDIIKKSKQVAKKLNLPLVVYIGEYPSYIDEVMNILGKG
                     DVVTPVYGNSTNGILKKSGTLRKSVINAKDRGVLFDVCHGLDQFDFKVFKKAIKQGLE
                     PDLISTDMHIKNMKDVNYSLLNVINKIMELGISLEKCIEKVTEYPTKIFDLHENKGRI
                     RIGGDGDFTIFTMEKSNDIIKDYNNNELILNKKLRLQYTVKSWMKSSEVYRHGYENTI
                     IN"
     gene            7150..7338
                     /locus_tag="L3M60_002876"
     CDS             7150..7338
                     /locus_tag="L3M60_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HBN6227516.1"
                     /translation="MKAFVDKDVCVACGACIGTCPDVFSMSDEGHSVAIEGEIPADLQ
                     DLCKEAEEGCPVSAITVK"
     gene            7488..8138
                     /locus_tag="L3M60_002877"
     CDS             7488..8138
                     /locus_tag="L3M60_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBN6227517.1"
                     /translation="MKKNYEIVLFDLDGTLTDPKEGITKSIQYSLNSFGIEEDLENLE
                     QFIGPPLYDTFKEYYKFEDKKAKEAVEKYREYFTDKGIFENKIYENIKEILEMLYKKG
                     KILLVATSKPTVFAETILRYFDIDRYFKYVAGSNLDGTRVNKNEVIQYVLDLCNVKDK
                     DKVIMVGDRKYDIIGAKKIGIDSIGVLYGYGSFEELSESEPTYLVENVESIKDILL"
     gene            8241..9179
                     /locus_tag="L3M60_002878"
     CDS             8241..9179
                     /locus_tag="L3M60_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN6227518.1"
                     /translation="MKSILIMGGSDFIGSALAKRLIKCGYQIDILTNGKKEIDYKGFK
                     KHLICDRKVRKDMENIITGRKYDYIYDMTAYTKEDVSNLIDFISMDSLKKYIVLSAGA
                     VYKDSGRNIKEENEKGENENWGKYGLNKKEAEDFVINSPIPYIIIRPTYIYGENNNLY
                     REYYFFEKIEKNEKIPVPKGKQVSNQFIYIGDLVKVLESIMKNPHVREAYNVTNPQLI
                     SWDDLIYTCGEVIGKEPIIKYVDMEKVEFRERTYFPFRNIDFNLDINKLIEHGLYIPN
                     VLLKEGLTATYKWFSANKPKMHDRKMNKVESVMQIV"
     gene            9205..10056
                     /locus_tag="L3M60_002879"
     CDS             9205..10056
                     /locus_tag="L3M60_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBN6227519.1"
                     /translation="MNSKIKYFLKFFILIFILIFVYSVAIEPSLLIVKKYNISENGTL
                     IKNDIIESKSESIKVVQISDTQIGSFYSTKNLKKVANKINTLNPDIIVFTGDLIDYSN
                     KNPNVDEITTILSSMNARLGKFSVFGNHDYMYKLPRYYRQIMKNSDFNLLVNENKKIK
                     LKDNKYINILGVDEILNGHPNIKYLESQIDNKNFNLLLAHEPDLVDMLSKYTMNLVLS
                     GHSHGGQIRLPIKGALVTPPYGRKYTKGFYDINGNHLYVSSGLGSTKLPFRFFNIPEI
                     IEFNIII"
BASE COUNT         4140 a          977 c         1805 g         3267 t
ORIGIN      
        1 ggaggatatt tatatggaaa tattcgcaat aggacattca aattatccat atgataagct
       61 tattgatatg ataaaaaaat atgacataaa ttgtgtagtt gacattagag aaactccata
      121 ttctaaatat aatactcaat acaataaaga ggcattcaac gaatctctta gaaattcagg
      181 atttgtatat atatacatgg gaaaagaatt tggagctaaa agaactaata aagatgtata
      241 tacacaagaa ggttatgcag attttgagaa agtagcaaaa gaagatattt ttttaaaagg
      301 aatagataga ttaaaaaaag gttgtcaaat gggttatagg atagttttat taggagctat
      361 gcaagaacct ataagatgcc atagaagtat tttggtagga aaaatattaa ataaagaagg
      421 ttttgatgta aaatatataa tgcatgaagg aaatttggca tatcagcaag atatagagga
      481 atcattattg gataagtatt ttgctgatag aaagcaactg agtattgaca atttattagg
      541 gagtgcatta acaagagaag aaatgataca agaaggctat aaactggcta ataaagaaat
      601 aggatataga actgagaaac taggtaaata aaaatagata agtagataaa acacagtact
      661 tatcttaaaa aaataatatt atgaatttat aatagctaag catgatgtgc caattaaaaa
      721 tattctagtt atttaatagt atcattaaat ttaggatatt ttttaatagc acatcatgtt
      781 gtagtgttaa aaaataaaat ctctaatatt gccataatca actgttctat taagagtagg
      841 atgactagat ataaatctaa ctgtaatttt tgaatttaac agacaaggat ttgaaattaa
      901 aaatactcta tctccattga caatagaagt aatagccact ggtaaaatat ttacaaatgt
      961 atcatatctg tttttggcaa tttgataagg ataatttcct acaaaaacaa atagattcaa
     1021 atcatatata gtatcagtaa accaagccca ctgaccataa actttgtcga tattcattgt
     1081 gcctaaacca atcgtatcaa agtatgttgc tatagttaaa taaatatttt ccatgttttc
     1141 taaatatgaa acagtatatc ttctgaaacg tatgggtgct gtgggagaaa taggataaac
     1201 catatttaca tttagattat tttcatttaa attaaccaaa gtatcacatc ctaaaattag
     1261 tttataaaat acaatattta aatggagacg attttgttac atctaaatgt aatattttaa
     1321 atgtggtagc cataaaaaat aataaacata gaataattat aaagatatac ggtgttcaaa
     1381 ttatattaaa caagatttat attatttgaa agcaatatat tatgatgcta ataatacaat
     1441 tttaaacttt gtattatcaa tttacagttc gataaactaa ttaaaaatta aatttatata
     1501 aagagggaaa agaggaatag ggatgtttat aaggaaaaat gttataaagt tagcaattcc
     1561 tataatgata gagcagacgt ttgtcatgct tttaggggta tttaatacaa tgatggcagg
     1621 tcatattggg gaagaagctg tatctgcagt tggaatggtt gattcaataa acaatatatt
     1681 tatatctgta tttgctgcat tatctgttgg agctactgtt gttgtagctc aacaaatagg
     1741 gaaaaaagat aatgaaaaag tgaatgaaac tattaagcag gctctagtgt ctggagcaat
     1801 attatccttt acaataacaa tattaatgtg gatttttaga acaggtgtaa ttaatttatt
     1861 ttatggttca gcagaagaat tggtaaaatc aaatgcaaaa ttatacatag agtttacatt
     1921 gtttacatac ccattcattg cgatacaaca aatagcaaat ggtattctaa ggggttgtgg
     1981 agatgtaaag aaacctatgt atgtgactat gtttatgaat atagtaaatg taacattagg
     2041 atatatactt atatatggtg ttgatttaaa tcctctagga ataaatttac aaacaccatc
     2101 ctatgggata acaggtgcag ctatgtctat tgccatagcc agaataattg gatgtatagt
     2161 tattgtaaga gccttattta aaggaaatga atttatatct ataggtaaaa tatttccatt
     2221 taagattgat gttgaaactc aaaagtctat atttaatata gggataccag ctggagttga
     2281 acaactactt tttaatgcag gtaaattgat tgttcaggta tttatagtta ctatgggaac
     2341 atcagcaatt gcatccaatg caataggaat gtcaattgca tccataataa atgtaattgg
     2401 gaatgctcta gctttgtctg ccacaacttt ggtgggacag tatataggac gtggagatat
     2461 taaaggagct aagagtactt tattatattt aactaaattt gcaacaattt gtttatttgt
     2521 agtaggagct atatttgtac caattgcacc ttggatttca agtttgtata ctcaaaatgc
     2581 tagtgtaatt gacatttcaa cacaattaat tcgaagtgat tgtttagcta tggtaatatg
     2641 gcctatatcc tttgtattat cagcagggct aaagggggct ggtgatacta gatatacaat
     2701 gatgactgca gtaataggta tgtggatttt tagaattttt acaggttacg tattgggggt
     2761 tgtattagaa tttggagtgt taggaatttg gataggtatg tatactgatt ggcttgtaag
     2821 aggaacttta tattgtttaa gattaagagg aacaaagtgg ctaaaacaca aaattgcata
     2881 gaatatatga aatcactttt agatagaaaa ctctatttag gagtgatttt ttatgtaatt
     2941 attatcataa ttataagtat gatatataag gtgattaaat tgcataaata aagcctataa
     3001 taatatggat actataaatt atatattgag cagaattaca taatttggtg ataaatatta
     3061 gaaatatata tctatagaaa ataatatata tgggtatatt attaaatata aaaaatatta
     3121 ggtatttatt taataataaa ggtgggtatt gttatgataa ataaaatagg aaaaataact
     3181 ctctatgtaa ataatcaaga ggatgcaaag ttgttttgga cagaaaaatt gaactttgtg
     3241 gtaacttatg aaaaggaaat gggtccaaat atgaaatggg tagaagttgc accaagtaaa
     3301 aatgaattta caacatttgt gttgtatgat aaaaatctaa tgatgaagca aaacaaagag
     3361 ataaatgtat ctcatccatc aataatttta agtacaagag acattaatgg aacttatgaa
     3421 caaatggcaa ataacgaggt tgaggttgga gaattaatgg aaatgccata tggaaggata
     3481 ttttctttta aagaccaaga tggaaatgac tatttggtca gagaggataa ataattttac
     3541 aaagtattaa atttactcaa tatctgatat aattagagac aataattatt ataaactagt
     3601 ccagaattag agttatataa ttaaaaatta agtgtaaatt ttgagtagag gtgataaaat
     3661 gtttaagaaa atggcagtac taaaagatat agcaactaaa ataggtcgta aaaaggctta
     3721 tgaactatta gaaatggttg aaggtaatga tgcctttgta gctgaggtaa agataaaaaa
     3781 gaatggaata gaatctaaaa aagaagaaat tatgttaaaa gataatcaaa aaataatatt
     3841 agagtatata gaaggttaat ttttaaatat atataagatg atatactaaa tttctaagat
     3901 gagtttgcat aaaactcatc ttttttactt ttcaataaat ttgttttatt atttaataca
     3961 cattttatct gcttagagtt gctgatatat aaaaattact gatataataa aagaatggat
     4021 attaatgtaa tattttacag aaaagagggg tattatgctt gaaataaaaa atctttcctt
     4081 taatgttgag tctaacagtg aggaactagg aataataaat gatgttagcc tttcatttga
     4141 gaagggaaaa cttattgtta tcacagggcc aaatggtgga ggaaaatcta caattgctaa
     4201 gcttattatg ggaattgaaa aagctactag tggtcagata attttagatg gagaagatat
     4261 aacaaatctt agtattacag aaagagcaaa aaaaggaata ggatatgcat ttcaacagcc
     4321 accaagaata aaaggtatga cagttgaaaa tttacttaca cttgctcatg gaaaaccatt
     4381 gtctactgat gtatgttgcc aataccttac agatgttgga ttgtgttcaa aagactattt
     4441 aaatcgtgaa gttgataact ctctttctgg gggagaaatg aaaagaattg agatagcaac
     4501 tctttttgct agagatttaa aggtttctat atttgatgaa cctgaagcag gaatagacct
     4561 ttggagcttt ggcaaactca atgaaagctt taagaaaata catgaagagt ctgaccagac
     4621 tataattata atttctcacc aagaaagaat attagagtta gctgatgaaa taattgttct
     4681 acaagatggt tcagtaaaaa gccatggaac aaaagaggct atattacctg aaatcgtgtg
     4741 tcaagttaat tcaagctgtg aattaatgaa agatatgaat taattagaaa ggaaggcaaa
     4801 ttaaatgaac aaaatagatt taaatctttt agaaaaagtt gctgaacttc gtggaactcc
     4861 attaggagca tacaatatac gtaaaaatgg gcaaggagta gagagacatt caactgaaaa
     4921 tgtaactata attcctaaaa ctgataaacc tggaatagat attatagtaa agccaaatac
     4981 aaaaggagaa aatgtacata taccagtaat agtaacagat actggtgtac aggatatggt
     5041 atataatgac tttgaaattg gagaaaatgc agatgttgtt ataattgctg gatgtggtat
     5101 acataactgt ggttgtgacg atagtgaaca taatggtata catagatttt tcttacgtaa
     5161 aggagctcgc ctaaaatatg tagaaaaaca ttatggtgaa ggtgatggta aaggaaagag
     5221 aattttaaat ccagttacta ttgttaatat ggatgaggat tcatattgtg agatggaaac
     5281 aattcaaatt aagggtgtag attcaactaa aagagaaatg gatgctaacc taggtaaagg
     5341 agctaaaata gttgtatttg agaaattatt aactcatggt aatcaagttg cagaatctaa
     5401 tatgaaggtt gtcttagagg gggaaaactc ttctgctcaa gtagtttctc gtacagttgg
     5461 gcaagataat tctaagcaaa aatttaatcc ttgtgttatt ggggattctc tttgttctgc
     5521 acatgttcaa tgtgattcaa taattatggg aaattctcaa attagtgcta tacctgaaat
     5581 tgctgcaaat cacccagatg ctcttttagt acatgaagca gctattggaa agattgcagg
     5641 agaccaaata ctaaagatga tgacattagg tcttactgaa gaagaagccg agcaacaaat
     5701 acttaattgc ttcttaaagt aaattaaagt ttttatttaa tttaattaat atagtataat
     5761 atagtgaata agtctaaaat atttttattt ttaggcttat tttatttatg taagaaaaac
     5821 tattagataa attatattgt aattaggatg tggaataaat gaaaaattta cttataagaa
     5881 atgggacaat aatatctcca acagatggta tcaaatttga aagaagagat atcctggttt
     5941 tacatgggaa aatatatgct attggattga accttaaaga aaaaataaga aaagaaaaat
     6001 tacttacaaa taaagagttc gagataataa atgctgataa tatgtatatt tccccaggat
     6061 ttatagatgt tcacacccat tgttataaga gaaaattacc tacaggtatg gattctgatt
     6121 caattggaat tgaaaaaggt tctacagtta tatttgatgc aggtacagct ggtcctttaa
     6181 actactatga ttttaaaaaa aagtatatgg atgagtgtaa aacagaagta tattcttttt
     6241 taaatgttac aaatgaggga ctaaatatat tgaatgaatc aacttcttta gatgttataa
     6301 attttgacaa ggttgagtca gttgtagaaa aaaatataga aaagataaag gggataaaag
     6361 taaaagcgag taaatttcaa tctaataaaa atggtataga tataattaag aaatcaaagc
     6421 aagttgctaa gaaattaaac ttacccttag tagtatatat aggtgagtat ccatcatata
     6481 tagatgaagt aatgaatatt ttgggaaaag gagatgtggt tacaccagta tatgggaata
     6541 gtacaaatgg aatccttaaa aaatctggta cattaagaaa atcagtaata aacgcaaagg
     6601 atagaggagt attgtttgat gtatgtcatg gattagacca atttgatttt aaagtattta
     6661 aaaaagcaat aaagcaaggt ttagagccag acctaataag tactgatatg catataaaaa
     6721 acatgaaaga cgtaaattat tctcttttaa atgttataaa taaaattatg gaacttggca
     6781 tatccttaga aaagtgcata gaaaaagtaa ctgaatatcc aacaaaaatt ttcgatttac
     6841 atgaaaataa aggaagaata aggattggtg gagacggtga ttttacaata ttcacaatgg
     6901 aaaagagtaa tgatataata aaagactata ataataatga actcatttta aataaaaaat
     6961 taagattaca atatactgta aaatcatgga tgaaaagcag tgaagtttat agacatggat
     7021 acgaaaatac tataataaat taaacaatag tgataaaata ataaatatat gtttaaattg
     7081 tcatatattg gataaatatc taaggaaaca acaattaaaa aataataatt ttaggggagg
     7141 tattttagta tgaaagcatt tgttgataag gatgtatgtg tagcttgtgg agcatgtata
     7201 ggaacatgtc cagacgtttt tagtatgagt gatgaagggc attcagtagc aattgaagga
     7261 gaaataccag cagacttaca agatttatgt aaagaagccg aagaaggatg tccagtttca
     7321 gctataacag ttaaatagag ttgtttaaat ttattttaaa aataaatata taagatttaa
     7381 ttgagtactt aagctatagt attagaaatt tctattacta tagcttttta aaatagataa
     7441 acttaaaata tagggtatta ttttttataa gtatggagga ctgaaatatg aagaaaaatt
     7501 atgaaatagt attatttgat ttagatggaa cacttacaga ccctaaagaa ggaataacaa
     7561 agtctattca gtattcttta aatagttttg gtattgaaga agatttggaa aacttagaac
     7621 aatttatagg accaccatta tacgatactt ttaaagagta ttataaattt gaagataaaa
     7681 aagctaaaga agctgttgaa aagtatagag aatattttac tgataaaggg atatttgaaa
     7741 ataaaatcta tgagaatata aaagagatat tagaaatgct ctataaaaaa ggtaaaatat
     7801 tattagtagc aacttctaag ccaacggttt ttgcagaaac tatacttaga tattttgata
     7861 tagatagata ttttaaatat gtagcaggta gcaatcttga tggtacaaga gtaaataaaa
     7921 atgaagttat acaatatgta ctagacctat gtaatgtaaa agataaagat aaagttatta
     7981 tggttggaga tagaaagtat gacataattg gagctaaaaa aataggcata gattctattg
     8041 gagtgttgta tgggtatggt tcttttgaag agcttagtga aagtgaacct acttatcttg
     8101 tcgaaaatgt agagtctata aaagatattt tgttataaat atcttttata gacaaaaata
     8161 ttacaaaatg gtgtcgcata tttaaaaata tgatattata gttaaggaag tcatttaaaa
     8221 aagaggagga tgcacttaaa atgaaatcta ttttaattat gggtggaagc gacttcatag
     8281 gttctgcact tgctaagcgt ttgattaaat gtgggtatca aattgatata cttacaaatg
     8341 gtaaaaagga aatagattat aagggtttta aaaaacatct aatttgtgat agaaaagttc
     8401 gaaaagatat ggaaaacatt atcacaggaa gaaaatatga ttacatatat gatatgacag
     8461 catatacaaa agaagatgta agtaatctga ttgattttat aagtatggat agcttaaaaa
     8521 agtatatagt attgtcagca ggagcagtat ataaagatag tggaagaaat ataaaagaag
     8581 aaaatgagaa aggtgaaaat gaaaactggg gaaaatatgg gttaaataaa aaagaggctg
     8641 aagattttgt tataaatagc cctataccat atataattat tagaccaact tacatatatg
     8701 gtgaaaataa taatttatat agagagtatt atttctttga aaaaatagaa aaaaatgaaa
     8761 agataccagt accaaaagga aaacaagtta gtaatcaatt tatatatatt ggagatttgg
     8821 taaaggtatt agaaagtata atgaaaaatc ctcatgtaag agaggcatat aatgtgacga
     8881 atccacagtt aatttcttgg gatgacctta tatatacgtg tggagaagtt attggcaaag
     8941 aacctataat aaaatatgta gatatggaaa aagtagagtt tagagaaaga acgtatttcc
     9001 catttagaaa tatagacttt aatcttgata taaataagct tattgaacat gggctttata
     9061 taccaaatgt gttactaaaa gaaggtctta cagcaacata taagtggttt tcagcaaata
     9121 agccaaaaat gcatgacaga aaaatgaaca aagttgagtc tgtaatgcaa atagtttaaa
     9181 aaaataaata agtaggtaga tattttgaac tcaaaaatta agtatttttt aaaatttttt
     9241 atcttaatat ttattttgat ttttgtatac tcagttgcaa tagagccgag tctattaatt
     9301 gtgaaaaaat ataatataag tgaaaatgga acattgataa aaaatgatat aattgagagt
     9361 aaatctgaaa gtataaaagt agttcaaatt tcagatactc aaataggaag tttttactca
     9421 actaagaatc tcaaaaaagt ggctaataag attaatactt tgaatccaga tataatagta
     9481 tttactggtg atttgattga ttactccaat aaaaatccaa atgtagatga aattacaact
     9541 attttatcaa gtatgaatgc taggttggga aaattttcag tattcggtaa tcatgattat
     9601 atgtataaac ttcctaggta ttataggcag attatgaaaa attccgactt taatctttta
     9661 gtaaatgaaa ataaaaaaat aaaattaaaa gataataagt atattaatat attaggtgtt
     9721 gatgaaatat taaatggaca tcctaatata aaatatctgg agagtcaaat agataataag
     9781 aattttaatt tattgttagc acatgaacca gatttagtag acatgcttag taaatataca
     9841 atgaatttag ttttatctgg gcatagtcat ggtggtcaaa taagactacc tattaaggga
     9901 gcattagtta ctcctccata tggaagaaaa tatactaaag ggttttatga tataaatgga
     9961 aatcatttat atgtgagttc aggtcttgga agcacaaagt taccatttag attttttaat
    10021 ataccagaga taatagaatt taatattata atctaacata aaatatttag aaaatatcat
    10081 tttgtttgtt ataatatgta aatgtatata ctgtaaaaat agattaaact agtagattga
    10141 atgaattaat agattaaatt aatatataaa atatttaagt tataattaa
//
LOCUS       DAFNNB010000139        10134 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0142, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000139 DAFNNB010000000
VERSION     DAFNNB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10134
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0142"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            12..779
                     /locus_tag="L3M60_002880"
     CDS             12..779
                     /locus_tag="L3M60_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227520.1"
                     /translation="MSKILRVKNIKKYYKSRGNITKAIDNISFEVESGEYIGIMGASG
                     SGKTTLLNCISTIDKTTDGNIYIGNKDITTMNEDELSKFRREELGFIFQDFNLLDTLT
                     AHDNIALSLSINGVNSKEIDNKIKKIANKLGVYEILNKYPYEMSGGQKQRIACARAII
                     TNPSLILADEPTGALDSKSAKLFLENLEVLNRELKATILMVTHDAFTASYCKRILFIK
                     DGKIFNELVRGNNTRQEFFDRIIKVISVLGGDDKHVF"
     gene            760..2889
                     /locus_tag="L3M60_002881"
     CDS             760..2889
                     /locus_tag="L3M60_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227521.1"
                     /translation="MINMFFKKNKSRRSRNKRKNNYVSRVSSESFKKLALNNVKKSLK
                     NYLIYFITLVFGVIILYTFNSLDNDIAILSSNALLSSSISFVRGIVSIFSILVCIIFA
                     FLIIYANNYLMKQRKKEFGIYSILGMDRRDINKLMFREMMIIGIFSLIIGIIFGVFVS
                     KGLSIFALKMLGISSSGFSFSMLTTIKTIVFFGIVLLLVNKFNKKTIKKYKLIDLLNA
                     NKKNEVSVAKGKKSNLILFFLSIVAIVLAYIIPLKMKISTFILGICLILILVGIYLFF
                     MSVSDFIMLKIKKHRFIYYKNLNPFIVSQISSQIKTMSMSITMICMLLFSSLIVIPFG
                     ISFGKYLIDDLQEATPYDVTLSKLYMNGDNIVEYSTKKNDIDSKTPSLKDELIENGFP
                     LTSFSKSTSDLRIYNLKNISQYDFISNDKDRTNELKDNKLHIVSISDYNFTRKQSGLK
                     EINLKDNEFAINCASEDYRPFYEKYIKSQPQNYITVNGVKLKLNQTSLYTNSYNTNTV
                     PLDLGTIIVPDAVVKDLNPIMTTLNIDYKERSSNLENKFMDSYYKFEEKINNSEAYSI
                     LYKSVIDGEKRALNTSFSFVAIYIGIILLISAGAILALQQLIESTVNKERFKLLSKLG
                     VNKKDMKKAIFIQVSILFAVPLILALINALFISKVFFVIMPFIAETGIITTMLLTLGI
                     VIVVYGIYFISSLLESLNIVNDSKEII"
     gene            3106..4317
                     /locus_tag="L3M60_002882"
     CDS             3106..4317
                     /locus_tag="L3M60_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227522.1"
                     /translation="MQIKIGKIIQALRKERNLTQEQLAKFIGVSTPAVSKWESGNSYP
                     DIELLPLLADFFNVSIDKLLNYKIDLSEEEVTKIYKELESIFAGIEVDLSTEEPKEEC
                     KRDLEYVKNLSNMYIEKYPKSYLLKLKICSLYQMYSYKFGKNDFNNNVRETTNILEDI
                     VRNTDDIQIKETALIILSNAYCMLEDYEKAELYLNMIHKPIGDTSVNLAMIYLNQNKL
                     EEAEILLQNKLFSNVFNLSLDCKGIINVYKYQYKELKKKLEYGDSNKKAIEKEMEYIK
                     NKLLGYANLSLEIKKMISEDKGAFFGIYMDYMELSLTFLFFNMKEEAKKALYSLKEIL
                     EKYPIHESLDVSQMRFFDKVKSKNSYTFNMYTNLLIMFSDDSYNELRGEPIFESIIEK
                     ILELEKLLKDK"
     gene            complement(4628..5149)
                     /locus_tag="L3M60_002883"
     CDS             complement(4628..5149)
                     /locus_tag="L3M60_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBN6227523.1"
                     /translation="MKFERLRTSKDKLYYKAMELYKTSFPFHEQRKSSLQAKIMENKE
                     YQFNLIYDKNQFVGIILCWETKSFIYVEHFCIFPEMRNNRYGQKTLELLNKRGKTIIL
                     EIDPPIDELSTRRKKFYERVKYKTNYYKHIHPPYQEKFNGHQLVVMSYPERLSKMEYD
                     KFNQYLKNHVMNF"
     gene            5511..6308
                     /locus_tag="L3M60_002884"
     CDS             5511..6308
                     /locus_tag="L3M60_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBN6227524.1"
                     /translation="MEKREKRIIISSLLSVSILMSLVSIYSILNKEDISLTVKGQEQK
                     VSSFKKTVEELLDEQGVKYNSEDKINPSLDTELKDDMKIKVVKVTKSKKEEIEKIPFD
                     TKHVNDSNLLKGKSKVYQEGKEGEKKLIYNLTYHDEKLVKKVLSKEVISKEPTTKIIK
                     YGTKEKVLIASRGANIRGSKHMKVVATAYAGDTITSTGTTPRWGVIAVDPRVIPYGTK
                     VYIPKLGMTFVAEDCGGAIKGNRIDIFMNSEGKASNWGRKSIDIYLH"
     gene            6418..7008
                     /locus_tag="L3M60_002885"
     CDS             6418..7008
                     /locus_tag="L3M60_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HBN6227525.1"
                     /translation="MKIVCLGDSLTYGFGVSRSNSWTNIVNKETRLEIVNKGINGDTT
                     SGMLVRFNEDVVKNSPDIVFIMGGTNDFIAGAGNEVINSNIMAMVHQSYSKNIIPIIG
                     IPLKPDIPNIREDWSCFTDFNIVSQKLESYSYWIKKFCMTFNTNFVDFYSEYNKNMEL
                     EGYKKLYFDGLHPTKEGHRIMADIFINSINKYIEEL"
     gene            7073..8770
                     /locus_tag="L3M60_002886"
     CDS             7073..8770
                     /locus_tag="L3M60_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HBN6227526.1"
                     /translation="MDYTKTYEEWIKGSYFDEDTKIELEKIKNNEKEIEDRFYKDLEF
                     GTAGLRGIIEAGTNRINKYTVRRATFGLANYILENTTKEETNRGVVIAHDNRHKSRQF
                     CIESANTLAACGIKAYIFDSLRTTPELSFAVRSLNAIAGIVITASHNPPEYNGYKVYW
                     EDGAQVMPEIANAITEKVNSIHDYSIIPTPSEANKNLVVLLDESQDTKFIEAVKSQII
                     RKDLVKNVGKSFKIVYTPLCGTGNIPIRRALKEVGFENIIVVPEEENPDPNFAGLDYP
                     NPEEKKALNRGILLAKEKGADLVIATDPDCDRVGVAVKTTIGEYALLTGNQIGGMLTH
                     YIIEGLKENNKLKENPTIIKTIVTSEFGADIAKANNVDVLNVLTGFKFIGEKIKLFEQ
                     NKNRSYVFGYEESYGYLVGTHARDKDGVVSSLLISEMAAFYYSKGMSLYEGLIELYEK
                     YGFFKEQTISLTLKGIEGVEKIKEIISYFRENQIDYINSVKVIDKKDYKNGIDNLPKS
                     DVLKYFLEDESWVAIRPSGTEPKLKFYIAVKGASDIEADKKIQGLKKYIDDMVGKLK"
     gene            8971..9810
                     /locus_tag="L3M60_002887"
     CDS             8971..9810
                     /locus_tag="L3M60_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HBN6227527.1"
                     /translation="MFGIDKKLIEYIIAKSNYSELSSKSAEVSFFLMLSIFPFLIFTI
                     SSIAYIPILHLNKYIALFRNMMPEGAFAVLSSIIVSAIDNRNLKFLAVSFVLTMWTFS
                     RAVKALIKGMNRAYKVKETRSFFKILSISFLFTIMLLVLIFLSMIFLVYGEKIGYFIF
                     NLIGLDEIFIKIWDILRYTVGIITIIVIFTLLYKYTPNKKLTIKESAPGAIFATFAWF
                     LVSFLYSYYTNYYANYEVIYGSIAEIIVLMTWMYFSSWSIVIGYEVNSRLYFRKIKHE
                     MLK"
BASE COUNT         4080 a         1005 c         1598 g         3451 t
ORIGIN      
        1 aggtaataga tatgagcaaa atattaagag taaaaaatat aaaaaaatac tataaaagta
       61 gaggtaacat tacaaaagct attgataata ttagctttga agttgaaagt ggagagtata
      121 taggtattat gggagcttca ggaagtggaa aaactacttt actgaattgt atttctacta
      181 tagataaaac gacagatggg aatatatata taggaaataa agatataaca actatgaatg
      241 aagatgagtt atctaaattt cgtagagaag aactaggttt tatatttcaa gattttaatt
      301 tattggacac acttacagca catgataata tagcattgtc tttatctatc aatggagtca
      361 attcaaaaga aatagataat aaaataaaaa aaatagcaaa taaattaggt gtttatgaaa
      421 tacttaataa gtatccatat gaaatgtctg gtggtcaaaa gcaaagaata gcatgtgcaa
      481 gagctataat aacaaatcca tcactaatac tagcagatga acctacagga gctctagact
      541 ctaagtcagc aaagttattt ttggaaaacc ttgaagtttt aaatcgtgaa ttaaaagcta
      601 ccatattaat ggttacacat gatgctttta cagctagtta ttgtaaaaga atattattta
      661 taaaagatgg taaaatattc aatgagttgg ttagagggaa taatactcgt caagaatttt
      721 ttgatagaat aataaaagta atatcagttc ttgggggtga tgataaacat gttttttaaa
      781 aaaaataaat caagaaggtc tagaaataaa agaaaaaata attatgtttc aagagtgtca
      841 agtgaatcat ttaaaaaatt agctttaaat aatgtgaaaa agagtctaaa aaattatttg
      901 atttatttta taacccttgt atttggtgtt attattttat acacttttaa ttctttagac
      961 aatgatatag caattcttag tagtaatgct ttattgagtt caagtatatc gtttgttagg
     1021 ggtattgtaa gtattttttc aattctagtt tgtattatat ttgcattttt gattatttat
     1081 gcaaataact atttaatgaa acaacgtaaa aaagagtttg gaatatacag tattcttgga
     1141 atggatagaa gagatataaa taaattaatg tttagagaaa tgatgataat aggcatattt
     1201 tcattaataa taggtattat atttggagta tttgtttcta agggacttag tatatttgcc
     1261 ttaaagatgt tgggcatatc atcatcagga ttttctttct caatgcttac aactattaaa
     1321 acaatagtat tttttggcat tgtactgtta ttagtcaata aattcaataa aaaaacaata
     1381 aaaaaataca agttaataga cttattaaat gcaaataaaa agaatgaagt ttcagttgca
     1441 aaaggaaaaa aatctaattt gatattattc tttttatcta tagtggcaat tgttttagca
     1501 tatataatac cattgaaaat gaaaatatct acatttatat taggtatatg tttaatttta
     1561 atattagttg gaatttattt gttttttatg tcagtatctg attttataat gttaaaaata
     1621 aagaagcata gatttatata ctataaaaat ttaaatccat ttatagtaag ccaaataagt
     1681 agtcaaatca aaacaatgag tatgtctatt actatgattt gtatgttatt attttcttct
     1741 ttgatagtaa taccatttgg tatatcattt ggaaaatatt taatagatga tttacaagaa
     1801 gcgacgcctt atgatgtaac attatctaaa ttgtatatga atggagataa cattgtagaa
     1861 tacagtacaa agaaaaatga tatagatagt aaaactcctt cacttaaaga tgaactaata
     1921 gaaaatggtt ttccactaac ttcattttct aaaagtacaa gtgatttaag gatatataat
     1981 ttaaagaaca taagtcagta cgattttata tcaaatgata aggataggac aaatgaactg
     2041 aaagataata aacttcatat tgtctcaatt agtgattata attttactag aaagcaaagt
     2101 ggtttaaaag aaataaatct aaaggataat gagtttgcaa taaattgtgc ctcagaagat
     2161 tatagaccat tttatgaaaa gtacattaag agtcagccac agaattatat aacagtaaat
     2221 ggagtaaaac ttaaattaaa tcagacaagc ttatatacca attcttataa tactaataca
     2281 gttcctttag atttgggaac tataattgtt ccagatgctg tagtaaagga tttaaatcct
     2341 attatgacta cattaaatat tgattacaaa gaaagaagta gtaatcttga aaataaattt
     2401 atggattcat actataaatt tgaagagaaa atcaataatt ctgaagctta tagtatttta
     2461 tataaatcag ttatagatgg cgaaaaaaga gccttaaaca cttcattttc ttttgtagca
     2521 atatatatag gtattatatt attaatatca gcaggtgcaa tattagcatt acaacaactc
     2581 atagaatcaa ctgttaataa agaaagattt aaattattaa gtaagcttgg agtaaataag
     2641 aaagatatga aaaaagctat ttttatacag gtatcaatat tatttgcagt acctttaata
     2701 ttggctctta taaatgctct gtttatatca aaagtattct tcgttataat gccatttatt
     2761 gcagagacag gtattattac aactatgtta ttaacattag gtattgttat cgttgtatat
     2821 ggaatttatt ttatttcaag tttacttgaa agtctaaata tagtaaatga tagtaaagag
     2881 ataatctaag tctttaaaaa gactatttat tgtgaatcta ttaattaagt tacacttaag
     2941 ttagcttagt taatagattt tatattttaa ttcaatgaaa gaatctttta gactaaaata
     3001 taaccaatag ttaaattatg aatattttat taactttgac tcaatttact tgtaagtact
     3061 ttttttatat acttaaatta caatagttat gctagggagg tatatatgca aattaaaata
     3121 ggtaaaatca tacaagctct tagaaaagaa agaaatctaa ctcaagagca attggcaaaa
     3181 tttataggag tatcaacacc agcagtatcc aaatgggaga gtggaaattc ttatccagat
     3241 atagaattat tgccattatt ggcagatttt tttaatgtat ctattgataa gcttttaaac
     3301 tacaagattg atttaagtga agaagaagtg acgaaaattt ataaagaatt agaatcaatt
     3361 tttgcgggaa ttgaagttga tttatcaaca gaggaaccaa aagaagaatg taagcgagac
     3421 ttggaatatg taaaaaacct ttctaatatg tatatagaaa agtatcctaa aagttatcta
     3481 ttaaagttaa aaatatgttc gttgtatcag atgtattcat ataaatttgg caagaatgat
     3541 tttaataata acgtaagaga aacaacaaat atacttgaag atatagtaag aaatacagat
     3601 gatattcaaa taaaagaaac agcactcatt atcttatcaa atgcatattg tatgttggag
     3661 gattatgaaa aggcagagct gtacttaaat atgatacata aaccaatagg agatacaagt
     3721 gtcaatttag ctatgatata tttaaatcaa aataagctag aagaagcaga aatattgctt
     3781 caaaataaac tgtttagtaa tgtattcaat ttaagtttgg attgtaaagg aataatcaat
     3841 gtatataaat atcaatataa ggaattaaag aaaaaattgg agtatggaga ttctaataaa
     3901 aaagcaatag agaaagaaat ggaatatata aaaaataagt tgctagggta tgcaaacctc
     3961 tctttagaaa ttaaaaaaat gattagtgaa gataaaggtg cattttttgg tatatatatg
     4021 gattatatgg aattatcttt aactttttta tttttcaata tgaaagaaga agccaaaaaa
     4081 gctttatata gtctaaaaga aatattagaa aaatacccta tacatgaaag tttggatgtt
     4141 agtcagatga gattttttga caaagtaaaa tctaaaaatt catatacctt caatatgtat
     4201 acgaatttat taataatgtt tagtgatgat agctataatg aacttagagg agaaccaatt
     4261 ttcgagtcta taattgaaaa gattttggag ttggaaaaat tattaaaaga taaataataa
     4321 ataaaaatct aatataagat ttaaagatta attaaattaa aaggagatgt gtcaaaacac
     4381 aaatttgaac catctccttt attacataaa aaaaatagaa aagggcttgt taagaagtat
     4441 tctaaaacca ttaaatcaaa tgattatatc aaaagaatag aaatatatta aaaataaaca
     4501 aatgaaaatt aagaagctga tatattttca tcattttaac aaaaaagaga cctatctcta
     4561 ttttgagaca gcctctttta tgtaattgtt atgaaacttt gcacaactca atagatagga
     4621 actatactta gaagttcatt acatgattct ttaaatactg attaaattta tcatattcca
     4681 ttttagataa tctttctgga taagacatta caactagttg atgaccatta aatttctctt
     4741 ggtatggtgg atgaatatgt ttataataat ttgtcttata ttttactctc tcatagaatt
     4801 tcttgcgacg agttgaaagc tcatcaattg gagggtcaat ttctaaaata atagtttttc
     4861 ctctcttatt tagcagttct aacgtttttt gtccatatct attattacgc atttctggaa
     4921 aaatacagaa atgttctaca taaataaagc tttttgtttc ccaacataga attatgccaa
     4981 caaactgatt tttatcataa attaaattaa attgatattc tttattttcc ataatttttg
     5041 cttgcaaaga agattttctt tgttcatgaa atggaaaact agttttatat aattccatag
     5101 ctttatagta taatttatct ttgctagttc ttaaccgctc aaatttcatt ttgttcctcc
     5161 taattacgaa aaattccgat ttatatatga atttatttta tcacagtcag catttgtata
     5221 caatgtgttt tcagttgtat attatttctc aattttattc cacttatgtc tacatctcat
     5281 gggctgaaat ataattgttt attgtgtaac ctcaaactat tatacaggtt ctgatggctt
     5341 aaaaacagca tacttataga atttttttat gtatgaaaat gattttaata gttttagaag
     5401 ttacaaaaaa gttacataaa tttggaaagc tataatatac atatataata taaggttaaa
     5461 actgtaataa ataatgttaa tttaaaattt gaacatggaa ggagattatt atggagaaaa
     5521 gagaaaagag gataataata tcatctttat taagtgtatc aattttaatg agtttggtaa
     5581 gtatatattc tatattaaat aaagaagaca taagtttaac tgttaaaggt caagagcaaa
     5641 aagtatcttc ctttaagaaa acagttgaag aacttttgga tgaacagggt gtaaagtata
     5701 attctgaaga taagattaat ccaagtttag atacagagct aaaagatgat atgaaaataa
     5761 aagttgttaa ggtaactaaa agtaaaaaag aagaaattga aaaaattccg tttgatacaa
     5821 agcatgtaaa tgatagtaat ttgttaaaag gaaaatctaa agtttatcaa gagggtaaag
     5881 agggagaaaa aaaactaatc tataatttaa cttaccatga tgaaaagtta gtcaaaaaag
     5941 tcttatcaaa agaagtaata tctaaggagc caactacaaa aattataaaa tatggaacta
     6001 aagaaaaagt actaatagca tcaagaggag caaatataag aggaagtaaa catatgaaag
     6061 tggttgctac tgcatatgca ggagatacaa taacatctac tggtacaact ccaagatggg
     6121 gtgttattgc agttgatcca cgtgtaatac catatggaac aaaagtatat atacctaagt
     6181 taggtatgac ttttgtggca gaagattgtg gaggtgcaat taaaggtaat agaatagata
     6241 tttttatgaa tagtgaagga aaagcctcaa attggggaag gaaaagcata gatatatatt
     6301 tacattaaat tagtgaatag aataacttta cttgaaagat aaaaaagtat aaaatatgaa
     6361 ttaactaaac tattagatta ttcttaaaac ataaaaatat atctaagggg ggataagatg
     6421 aaaatagtct gtttaggaga cagtttaaca tatggatttg gagtatcacg aagtaattca
     6481 tggacaaaca ttgtaaataa agagactcga ttagaaatag taaataaggg tataaatggt
     6541 gatacaacaa gtggtatgtt agttaggttc aatgaagatg ttgtaaaaaa ctctcctgat
     6601 atagttttta taatgggtgg taccaatgat tttattgcgg gtgcaggaaa tgaagttatt
     6661 aattctaata taatggctat ggtacatcag tcatattcaa aaaatattat acctataata
     6721 ggtataccat taaaaccaga tattccaaat attagggaag attggagttg ttttactgat
     6781 ttcaatatag tatctcaaaa gttagaatca tatagctatt ggataaaaaa attctgtatg
     6841 acatttaata ctaatttcgt agatttttat tcagaatata ataaaaatat ggaactagaa
     6901 ggatataaaa aattatattt tgatgggtta catccaacta aggaaggtca tagaataatg
     6961 gcagacattt ttattaattc aattaataag tatatagaag agttatagtt acatcaaaat
     7021 acttgtttat gtgtcagttt caataaatac atacagtaga gaggggttta ttatggatta
     7081 tactaaaact tatgaagagt ggataaaggg ttcatatttt gatgaagata caaagataga
     7141 gttggagaaa ataaaaaata atgaaaaaga gatagaggat agattctata aggatttaga
     7201 atttggaact gcgggtctta gaggaataat tgaagcagga acgaatagaa taaataagta
     7261 cacagttaga agagctactt ttggattggc aaactatata ttagaaaaca caacaaaaga
     7321 agaaacaaat agaggagtag taatagctca tgacaataga cacaagtcta gacaattctg
     7381 tatagaatct gcaaatactt tggcagcatg tggtataaaa gcatatatat ttgatagttt
     7441 gagaactact ccagaattat cttttgctgt tagaagctta aatgcaatcg ctggtatagt
     7501 cataacagca agtcataatc ctccagaata taatggatat aaggtatact gggaagatgg
     7561 agcacaagtt atgccagaga ttgcaaatgc aataacagag aaagtaaata gtatacatga
     7621 ttatagtata ataccaaccc caagtgaagc taataaaaat ttagtagttt tattggatga
     7681 atcacaagat actaaattta tagaagcagt aaaaagtcaa attataagaa aagatttagt
     7741 taaaaatgta ggtaaaagct tcaagatagt ttatacacct ctttgtggaa ccggaaatat
     7801 tccaataaga agagcattaa aagaagtggg ttttgaaaat ataattgtag ttccagagga
     7861 agaaaatcca gacccaaatt ttgcagggct tgattatcca aatccagaag agaaaaaagc
     7921 tttaaataga ggtatattac tcgcaaaaga aaaaggagca gaccttgtaa tagcaactga
     7981 cccagattgt gatagagtag gtgtagcagt aaaaactact ataggtgagt atgctttact
     8041 tacaggaaat caaattggtg gtatgctaac acattatata atagaaggat taaaagaaaa
     8101 taataaatta aaagaaaatc ctacaataat aaaaaccata gttacttcag aatttggagc
     8161 agatatagct aaagcaaata atgttgatgt attaaatgtt cttacaggtt ttaaatttat
     8221 tggtgaaaaa ataaaattat ttgaacaaaa taaaaataga agttatgtat ttggatatga
     8281 agaaagttat ggttacttag ttggtactca tgcgagagat aaagatggtg tagtttcttc
     8341 gttattaata tctgaaatgg cagcctttta ctattcaaaa ggtatgagtt tatatgaagg
     8401 tttaatagaa ctttatgaaa aatatggatt ttttaaagaa cagaccatat ccttgacttt
     8461 aaaaggtata gaaggtgtag aaaagataaa agaaataata tcttacttta gggaaaatca
     8521 aattgattat ataaatagtg ttaaagtcat tgataaaaaa gattataaaa atggtataga
     8581 taatcttcct aaatctgatg ttttaaaata ctttttggaa gatgaatctt gggtagctat
     8641 aagaccatct ggaacagaac caaaacttaa attttacata gctgtaaaag gagctagtga
     8701 tattgaagca gataaaaaga ttcaaggttt aaaaaaatat atagatgaca tggtcggaaa
     8761 attaaaataa aatattatta ttttataata atatttattt agctgaaaat tataatttac
     8821 tttcattgat tagaatatgt aaatgttagt agaattgtta ttttcagctt ttatttgtgt
     8881 tatttttact taaataatta tattatagta tgaatctgag tataattaat attgtataat
     8941 gaaattaatt tgatagaaaa ggggatattt atgtttggta tagataagaa attgattgaa
     9001 tatataattg caaaatccaa ttacagtgaa ctaagttcaa agtcagcaga agtatctttc
     9061 ttcttaatgt tgtccatatt tccttttttg atatttacaa taagttctat cgcttatata
     9121 ccaattcttc atttaaataa gtatatagct ttgtttagaa atatgatgcc agaaggagct
     9181 tttgccgtat tatcttcaat catagtttct gcaatagaca atagaaatct gaaattttta
     9241 gcggtcagtt ttgttcttac tatgtggaca ttttcaagag cagtaaaagc ccttattaaa
     9301 ggaatgaata gagcttataa agttaaagaa actaggtctt tttttaaaat tttatctata
     9361 tcatttttat ttacaataat gcttttggtt cttatttttt tatctatgat atttctagtt
     9421 tatggggaaa aaataggtta ttttattttt aatctcatag gactagatga gatatttata
     9481 aaaatatggg atattctaag atacactgta ggtataataa caataatagt gatatttaca
     9541 cttttatata aatacactcc aaataaaaag ctaactatta aagaatctgc accaggtgct
     9601 atttttgcta cttttgcttg gtttttagta tcatttttat attcttatta tacaaattac
     9661 tatgctaact atgaagtaat ctacggaagt atagctgaaa taattgtact tatgacctgg
     9721 atgtatttta gtagttggtc tatcgttatt ggttatgaag taaattcaag attgtatttt
     9781 agaaagataa aacatgaaat gttaaagtaa agcattaaaa taaaggatat tatttaatat
     9841 tataaatgta attgaattaa taaaaaatat tgacatttaa gttaaaaagt ctagaatttt
     9901 tctgggcttt ttttattttt agtacaactt gacaaacttt atttaatggg atagttttaa
     9961 tttataatca tctttgaaaa gatgactatt gataagcaag agatgaaatt ggtaaaagta
    10021 taaaaaaata tactaaataa agattatagt cctaatgagt taatgtctgt gttttcaaat
    10081 aatgcagtat ttagtcatgt gtttttatat gcgcctgtat tcctaattac caat
//
LOCUS       DAFNNB010000140        10126 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0143, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000140 DAFNNB010000000
VERSION     DAFNNB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10126
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0143"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(122..1153)
                     /locus_tag="L3M60_002888"
     CDS             complement(122..1153)
                     /locus_tag="L3M60_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227528.1"
                     /translation="MKIEMGESLLYSWLRHVKECQIVQTNWKVSSKWDLKNADKILEI
                     MEKTKQYFEQKYGYPIYKNNSLEQLIMQAEVDVLGISIDNGRNHIYAIDVAFHEAGLN
                     YGSKNETVTRVIKKCIRTAMCIYGYMDLDEAEIIFASPKINPAIITELEPKMAEVNDL
                     FKSLGLRFKTRLIANEEFNGKILEPILIASDGISDTSELFLRSYQMYSMFSYSRDVVK
                     QQRIKKSNSLKDAVELNLSDETLKELKIGKLVQVTMKHLFEEEKISPEEIDKMTQSSY
                     SKQVFNENKPVLKEVPKDGNINELKKDEKNYNRYYAYTISIYGKQYLLVSQWIEDLHR
                     SYYESWVIK"
     gene            complement(1304..1954)
                     /locus_tag="L3M60_002889"
     CDS             complement(1304..1954)
                     /locus_tag="L3M60_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227529.1"
                     /translation="MEVIRNMTNVSSLEKEDEIVEILSSKENIRVAIEIERGLKLCKT
                     QMIKKVLEEIEKRMDKKFEDKYKLPYYSYKENNYALVNNYYNKKSSTYPAINYFIKSL
                     DKEDVDLLLRIEIDHHIFVGFCTLYKEKPSGKILSDDEIKELINDDGSRTNGWWICWE
                     YIYNNTMECPNFKNFNDAYFDLFDDNKFDEFMDLCEKRILSILGKLKDKQCINTFI"
     gene            2147..2605
                     /locus_tag="L3M60_002890"
     CDS             2147..2605
                     /locus_tag="L3M60_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBN6227530.1"
                     /translation="MSVDTLRNQFIKIVIEIYEDLTEFIFSKDIQTDKNLDFILKEPK
                     YIPNGFKEVEKENTLQGIFIRYENKNGSEIRYRSNQIDSNTVILDTENAKVTDIMVND
                     YEAKYIVKDNSITIFWNDEENVYTIYFDYDNKNDLNKYKDNLIDIAENIK"
     gene            2680..3102
                     /locus_tag="L3M60_002891"
     CDS             2680..3102
                     /locus_tag="L3M60_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227531.1"
                     /translation="MYLKKVIVAGLCTLTCLLPLNVQAFEDTSLNTNDYNQTNDNDLF
                     MIYIKGAGSNLSIRSGKAHISCYVDGKIGSLTSITARLQQKKSGKWVTISTWSSSGKT
                     SSTLSREYRVSKGYSYRILTTIKAGNESRNITSNVTNY"
     gene            3132..3818
                     /locus_tag="L3M60_002892"
     CDS             3132..3818
                     /locus_tag="L3M60_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227532.1"
                     /translation="MKYFKKVIALGLSIALLIPVISINALEKKDTNVNNQIINVIESN
                     VSYTKEGIEVSSDLKNDLKEINLEDIKEQMIEQGIDSKFINNFNSDVIYSLVKENIDD
                     INEGVKNKTLKITPEEDIINNTDNSFYVQGGSTYTQKFSWGVRQNKSTAAANRWVYDL
                     NKSSQGFVATGAVSVLLGSMGAPIGFVSGLSAAYMGGLANKVSYVNSRNNRGITVDIN
                     WWLTYAVKNQ"
     gene            3966..4778
                     /locus_tag="L3M60_002893"
     CDS             3966..4778
                     /locus_tag="L3M60_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HBN6227533.1"
                     /translation="MNYSDMHEISSGKSQKIALIDSGISKFQKVYKNINLTNSNNDYD
                     TNGHGTMMYSIIKGYNKNNQTIEGISPDSRIILIKVMNDDESINPNVIEDAIDYALNE
                     KVDIISLSLGSTKTDINIKQKIDKANLKNITVVSSAGDYKQGFLLFPASLNNVISVGS
                     IDDRRDISTLTNAPQKTLINAPGEKIKVIDNNNNVFYGKGTSEATAIITGYIALMRDT
                     NISSTQILTNEKIMNLLSQINKNEKTYLGALKEIRKKVSVSTGAFFSFDLSY"
     gene            5360..5521
                     /locus_tag="L3M60_002894"
     CDS             5360..5521
                     /locus_tag="L3M60_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227534.1"
                     /translation="MIDNKYIFRGKNIKNYIKFKDITGANKQDSLIYLKALSIEREEY
                     LINRLKSFF"
     gene            complement(5723..6079)
                     /locus_tag="L3M60_002895"
     CDS             complement(5723..6079)
                     /locus_tag="L3M60_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HBN6227535.1"
                     /translation="MNIIYEKEFTFDNKVREFISLGNNYKFEFSTSKSPKTLIKYFMD
                     YNKISSNKLESFKEKYSFKKNELLSLQEILKNKFTEDDKLESLKLSKANLENELKLGN
                     SIIKKSNSNHNMVEKK"
     gene            complement(6195..6425)
                     /locus_tag="L3M60_002896"
     CDS             complement(6195..6425)
                     /locus_tag="L3M60_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6227536.1"
                     /translation="MSYAETKACACSDDSILKLCEINREIQIIEINKKAFLNQNLLTL
                     NDIKSTENSIDALKISISKLEEDIKTYFSNFF"
     gene            complement(6598..8811)
                     /locus_tag="L3M60_002897"
     CDS             complement(6598..8811)
                     /locus_tag="L3M60_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HBN6227537.1"
                     /translation="MNQSYHNIKNELLSKQLAYIDRLTGYLVDRDNYLSGYVKEKLKI
                     AKEASIKDNTFLVNVKALEANQPKYETDVFFSIDSTWISSSIKSDFIEKTLELKENSI
                     QLLYSTQLGYSVKCSDIIPISKNEFTWGTKRRKSLYIVEAELNMKTITVTDKEYQNGK
                     EVSVKNLEETQLAMNILNKWLIAFHNYVTSNSYIHKELLDRYNEMFIDYKEKEYKNIL
                     NDVRINPNIKLREHQLKAASRIITSDRNTLLCHSVGSGKTYTMITAAQELNRISKIDS
                     KKGNKNLFVIPNSLCESGQFAKEYFNLYPQANILATTSKDFSKSNRRKIIAKIVTCNW
                     DSIIIPHSVLGLIPLEADTELKLLDLYLKELNDTLKYYKKECPDLSTKRLETMKENYL
                     NRIKELNDMHKDDALLSFEKLGITNLFIDEAHNFKNLYFNTTMQVPGISTLKTKKTQD
                     LYNKLRYFKSIHGNKGVTFATATPISNSICEMYTLLTYLNKDTLKYYKVDTFNAWASK
                     FGSIISSMEVDPTGQGFRFQQRFAKFCNVPELVNIFRQIADVVNICDINDLDIPQIKG
                     GKPIIEIIKPTNQMKEYIDSLVKRAEKIHEGPYDPIIDNMLNVTTDGRLMAVSPALVG
                     IDGILPKIIKVGENIASLYHEDKISTHLVFCDLGTPNGNSHNVYNDIKNELLKNNVLE
                     KDIAYIHDANTPEKRKKLIDDFNNGIIRILIGSTSKITSSCRCSLETFINFTKRR"
     gene            complement(9307..9873)
                     /locus_tag="L3M60_002898"
     CDS             complement(9307..9873)
                     /locus_tag="L3M60_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227538.1"
                     /translation="MNNLFYDKLNFDDKLIIKNNGVIRNNDLYFLKYKNDTAGILIYN
                     IINDKFSFKQNKNYKGMNPFPITNIFKNLDSDKWDEFIRIWIEERVIQECRPEVPFIL
                     KEFKLGYYDVWDICRSNLAMSVEDFFWITKDNTLRYEDFNIRYCTLNNITPIYKPPFP
                     IVIYPNYFEREKIKSGETWKYELKNTNS"
BASE COUNT         3639 a         1277 c         1216 g         3994 t
ORIGIN      
        1 aatgtgtgta atagaagatg aatacattaa gttaaaaatg tttaatgtac aactaactat
       61 tttattaaat ttaactttta tgttattgca tttttcttag atttagcaag agcattataa
      121 tttatttgat aacccaactt tcataatatg aacgatgtaa atcttctatc cattgtgata
      181 ctaataaata ttgctttcca taaatggaaa tagtgtaagc ataatatcta ttgtagtttt
      241 tttcatcttt tttcaattca ttaatattac catctttagg aacttctttt agtacaggtt
      301 tattttcatt aaacacttgc tttgaataac tggattgtgt catcttatca atttcttcag
      361 ggctaatttt ttcttcttca aaaagatgtt tcatagttac ttgaactaat ttcccaatct
      421 ttaattcctt taatgtttca tctgacaaat ttaattcaac tgcatctttt aaagaattag
      481 atttttttat tctttgttgt tttacaacat ctcgactata tgaaaacata ctgtacattt
      541 gataacttct taggaaaagt tctgatgtgt cagagattcc atcactagca attaagattg
      601 gttctaggat tttaccatta aattcttcat tcgcaattaa tctagtttta aatctcaatc
      661 ctaaagactt aaataggtca ttgacttctg ccatcttagg ttctaattca gttataatag
      721 ctggattaat ctttggagat gcaaatataa tttctgcttc atctaagtcc atataaccat
      781 aaatacacat tgctgtacgg atacatttct ttataaccct tgtaacagtt tcatttttag
      841 atccataatt aagtcctgct tcatgaaaag ctacatctat agcataaata tgatttcttc
      901 cattatctat tgaaatacct aatacatcaa cttcagcttg cataattaat tgctccaatg
      961 aattattttt atatatagga tatccatatt tctgctcaaa atattgttta gttttttcca
     1021 taatttctag tattttatca gcattcttta aatcccattt agatgacact ttccagtttg
     1081 tttgcactat ttggcattct ttaacatgac gtagccaaga atacaacagt gattctccca
     1141 tttcaatctt catagaatta ccatcctttt cagtctatta tctattaatt gttaaactat
     1201 atctaaattc ttccatcata tcttcaaatc atgtaatctc ttatttgtta tcaaaatcct
     1261 tgagtcttat acatttacat accttgttag gtaatattct attttagata aaagtattta
     1321 tgcattgctt atcttttagt ttacctaata ttgacaaaat tcttttttca cataaatcca
     1381 taaattcatc aaatttatta tcatcaaata aatcaaaata agcatcatta aaatttttga
     1441 aattaggaca ttccatagta ttattataaa tgtattccca acaaatccac catccattag
     1501 tacgtgaacc atcatcattt attaattctt ttatttcatc atcacttaga atctttccgc
     1561 taggtttttc tttatataac gtacaaaaac ctacaaatat atgatgatct atttcaattc
     1621 ttagtaaaag atcaacatct tctttatcta gactttttat aaaataatta attgctggat
     1681 atgtagagct tttcttatta taataattat tcactaaagc ataattattt tctttgtatg
     1741 agtaatatgg taatttatat ttatcttcaa atttcttatc catacgtttt tcaatttctt
     1801 ctaaaacctt ttttatcatt tgtgttttac ataactttaa tcctctttca atttctattg
     1861 ctactcttat attttcttta gatgataaaa tttctactat ctcatcctct ttttctagtg
     1921 atgatacatt tgtcatattt cttattacct ccataaattg cagtataatt tctcttattg
     1981 gagctatttt tatagtttca tgatgtgaga cacatttatt tagccaattt aatatatcta
     2041 tttcaaaaga tatttgagta acttctttat taaatatact attatttatt taaaacgagt
     2101 tgcagttgtg tttttaataa ttataactgg attattcaca ataactatga gtgttgatac
     2161 ccttagaaat caatttatca aaattgtaat agaaatttat gaagatttaa cagaatttat
     2221 tttttctaaa gatattcaaa ctgataagaa tcttgatttt atattaaaag aacccaaata
     2281 tataccaaat ggtttcaaag aggttgaaaa agaaaacaca ttacaaggta tctttattcg
     2341 ctatgaaaac aagaatggct ctgaaataag atatcgttcc aatcaaattg attcaaatac
     2401 agtaatatta gatacagaga atgcaaaagt cacagatatt atggtaaatg attatgaagc
     2461 taaatatatt gttaaagaca atagtattac aattttttgg aatgatgaag aaaatgtata
     2521 tacaatatat tttgattatg ataataaaaa tgatttaaat aagtataaag ataatcttat
     2581 agatattgct gaaaatataa aataattttt ttcaaaaagt gtcacaaatc accttttttt
     2641 tcgttatatg agtatataag aaaaaaggag gtgatatttg tgtatttaaa aaaagtgatt
     2701 gtagcaggat tatgtacatt aacttgccta ttacctttaa atgttcaagc ttttgaagat
     2761 acttcattaa atactaatga ttataatcaa acaaatgata atgatttatt tatgatttat
     2821 attaaaggtg ctggtagtaa tttgagtatt cgttctggaa aggctcatat atcttgttat
     2881 gttgatggta aaattgggag tttaacatca attacagcaa gactacagca aaaaaaatct
     2941 ggtaagtggg taactatatc aacatggtca agctcaggaa aaacttcaag tactttatct
     3001 agagaatata gagtatcaaa ggggtattct tatagaatat taacaacaat aaaagctgga
     3061 aatgaaagta ggaatataac aagtaatgta acaaattatt aatttaatta aaatttagga
     3121 ggaatattat tatgaaatat tttaaaaaag taattgcatt aggactatca attgcattgc
     3181 ttattccagt aatttcaatc aatgcattag aaaaaaaaga cacaaatgta aataaccaaa
     3241 taataaatgt tatagaatca aatgtatctt ataccaaaga gggtattgaa gtttcttctg
     3301 acttgaaaaa tgatttgaaa gaaattaact tagaagatat taaagaacaa atgatagagc
     3361 aaggaattga ttcaaaattc ataaataatt ttaatagtga cgtaatatat agtttagtta
     3421 aagaaaatat agatgatata aatgaaggag taaaaaataa aactcttaaa attacacctg
     3481 aggaagatat tataaacaat acagacaact cattttatgt acaaggaggt tctacatata
     3541 ctcaaaaatt ttcatgggga gtaagacaaa ataaaagtac tgcagcagca aatagatggg
     3601 tttatgattt gaataagtct tcacaaggat ttgtagcaac aggagcagta agtgttcttt
     3661 taggttctat gggagcacca ataggatttg tatctggttt atcagctgca tatatgggag
     3721 gtcttgcaaa taaagtatct tatgtaaatt cacgaaataa tagaggaata actgtggata
     3781 taaactggtg gttaacttat gcagtaaaaa atcagtaaaa attaggtgaa attaatgagt
     3841 gaaaaacaaa acttaaatta taaaaaaaaa atcttaaaac aataatattt atatcttgtt
     3901 taataatttt atctatattt atagttttta ataatataaa atctgactgt tggtatgtta
     3961 attatatgaa ttatagtgat atgcatgaaa tatctagtgg gaaaagtcaa aaaatagcat
     4021 tgatagattc tggtatttca aaatttcaaa aggtttataa aaatataaat ttaactaata
     4081 gcaataatga ttatgataca aatggtcatg gaacgatgat gtattcaatc ataaaaggtt
     4141 ataataaaaa taatcaaacg attgaaggaa tatctccaga ttctagaata atattaatta
     4201 aagtaatgaa tgatgatgaa agtattaatc ctaatgtaat agaagatgct atagactatg
     4261 cattaaatga aaaagtagat attattagtt taagtttagg atccacaaaa acagatatta
     4321 atataaaaca aaaaatagat aaagctaatc ttaaaaatat aacagttgta agttcagcag
     4381 gtgattataa acaaggattt ttgctatttc cagcatcttt aaataatgtt atttctgttg
     4441 gtagcataga tgatagaaga gatatttcaa cattgacaaa tgcacctcaa aagacattaa
     4501 taaatgcacc tggagaaaaa ataaaggtga tagataataa caataatgtt ttctatggaa
     4561 agggaacatc agaagcaaca gctattatta caggttacat agcgttgatg agagacacaa
     4621 atatttctag tactcaaatt ttaactaatg aaaaaattat gaatttatta tcccagataa
     4681 acaaaaacga aaaaacatat ttaggagcat taaaagaaat cagaaaaaaa gtatctgtaa
     4741 gtacaggtgc ttttttctct tttgatttat catattaaaa atagtgagaa atattttact
     4801 tgaataaatt ctatttgttt ataattatag tatgcattta aaatgagggg gaaaatagta
     4861 tgttctataa caagcaaatt aaaatattaa ctttattaat ttctatctta atagttttaa
     4921 tatcagcact ttcttgtact aatattatta aaaattttaa tttattaaaa ttttgtttaa
     4981 tattatttgg agtggaacaa attttactag cttataattc ttataagctt aataaaaatc
     5041 taatggagac acttttatta tgtattattg gaataatcct tataggtatt tctattgtaa
     5101 ttatgtgaat ataaataaca cgttttgaat ggaaaattat attaatttag ttcactaata
     5161 ttacatcttt aatgcaagta gagtaaagta ttttgaataa cataggtcaa tataaattgt
     5221 aggagtgatt ttcaataata tgtatattca aagtaacttt tctatttttg ttacttgcag
     5281 gtttttatct tgaacaaacg tataaatttt aataaaatat aaaaataaac tttatatttc
     5341 aattacagat gatgatattt tgattgataa taaatatatt tttagaggca agaatattaa
     5401 aaactatatt aaatttaaag atataacagg tgcaaataaa caggatagtc ttatatatct
     5461 aaaagcttta agcattgaga gagaagaata tctaataaat agactaaaat catttttctg
     5521 atttcatata ttttaaagtt ttaattaaaa gggttgcaga tattgagtta caaggtcttt
     5581 tttgtaatat aaaaaacacc actttgtgct cattacaaat ttcaatactt tctgtatcta
     5641 aaagtattga aaatactcaa tttattttgg aattttatac tacacatgca taaaacaatt
     5701 ttaaaacaat tgcaatatta attcattttt tttctaccat attatgattt gagttgcttt
     5761 ttttaattat agaatttcct aattttaatt cattctcaag atttgctttt gaaagtttaa
     5821 gagattctaa tttatcatct tctgtaaact tattctttaa aatttcttgg agagataaga
     5881 gttcattttt cttaaatgaa tatttttctt taaaactttc taatttattt gaactaattt
     5941 tattataatc catgaaatat ttaattagtg ttttaggact ttttgatgta gaaaattcaa
     6001 atttatagtt attacctaga gaaatgaatt ctctaacttt attgtcaaat gtaaattctt
     6061 tttcataaat tatgttcaag ccatatattt cacctaaaat tcctactttt ccattattta
     6121 ttatatctga cagagcttct atagcatctt taatatcaat ataagtttca ttatctaatg
     6181 ttatcttaaa ttcattaaaa aaaattagaa aaatacgttt taatatcttc ttctaattta
     6241 gaaattgata tctttaaagc atctattgaa ttttcggttg attttatgtc atttaaagta
     6301 agtaaatttt gatttaaaaa ggctttttta tttatttcaa ttatctgaat ttctctattt
     6361 atctcacata attttaatat gctatcatca gaacaggcac atgcctttgt ttcagcataa
     6421 ctcataagct gattacctat gtcttcagca gtcctagaag atgaagaatt attaagaatt
     6481 tgagctatat attttgcctt tatttctatt gtctgtcacg aatatgcatc aaaagaacct
     6541 tcaactacat acctaaaaat atagacctct ttattcaaat ttccttgcct taatattcta
     6601 ccttctcttt gtgaaattga tgaaggtttc caaggaacat ctacatgatg aagtgatttt
     6661 agaggtagaa ccaattaata ttctaataat accattattg aaatcatcaa taagtttctt
     6721 tcttttttct ggagtattag cgtcatgtat gtatgctata tctttttcta aaacattatt
     6781 ttttagaagt tcatttttta tatcattata aacattatgt gaatttccat taggagttcc
     6841 tagatcacag aatactagat gtgtacttat tttatcctca tgatataaag aagctatatt
     6901 ttctccaact tttattatct ttggtaatat accatcaatt ccaactaatg cagggcttac
     6961 agccatcaaa cgaccatctg tagttacatt taacatgtta tctattattg gatcatatgg
     7021 gccttcatga attttttctg cacgttttac aagagaatct atatattctt tcatttggtt
     7081 tgtaggtttt ataatttcta ttataggttt tccacctttt atctgaggaa tatctaaatc
     7141 atttatgtca cagatgttaa ctacatcagc aatttgtctg aatatattaa ctaattctgg
     7201 aacattacag aatttagcaa atctttgctg aaatctaaac ccttgaccag taggatctac
     7261 ttccattgag gatataattg aaccaaattt acttgcccaa gcattgaaag tatctacttt
     7321 gtaatacttt aatgtatctt tatttaaata agtgagtaaa gtatacattt cacatatact
     7381 attagaaata ggtgtagctg tggcaaatgt aacaccttta tttccatgaa tagatttaaa
     7441 atatcttagt ttgttgtata aatcctgtgt ttttttagtt tttaaagttg atataccagg
     7501 gacttgcata gttgtattaa aatataaatt tttgaagttg tgagcttcat ctatgaataa
     7561 gtttgttatt ccaagctttt caaatgaaag aagagcatcg tctttatgca tatcatttag
     7621 ctctttgatt ctatttagat aattttcttt cattgtttca agtcgttttg tagaaagatc
     7681 agggcattct tttttgtaat atttaagagt atcatttaat tcttttaagt ataagtctaa
     7741 taattttaat tctgtatcag cttctaaagg gattaatcct aataccgaat gtggaattat
     7801 tatactatcc cagttgcatg tcacaatttt agctataatt tttcttctat tggatttaga
     7861 gaaatccttt gaagttgtag ctaatatatt cgcttgagga tataaattaa aatactcttt
     7921 agcaaattgt ccagattcac aaagtgaatt tggtataaca aataaattct tattaccttt
     7981 ctttgaatct attttagaaa ttctatttaa ttcttgtgca gctgttatca ttgtataagt
     8041 ttttccagaa ccaacactat gacatagaag agtattacga tcagaagtaa ttattctact
     8101 agccgctttt aattgatgtt ctcgtaattt tatattagga tttattctaa catcatttaa
     8161 tatattttta tattcctttt ccttataatc tataaacatt tcattgtatc tatcaagtaa
     8221 ttctttatga atataactat tacttgttac atagttatga aaagctataa gccatttatt
     8281 taatatgttc atagcaagtt gagtttcttc caaattttta acagaaactt ctttaccatt
     8341 ttgatattct ttatcagtga cagttatagt tttcatattt aattctgctt caacaatata
     8401 caaagatttt ctcctttttg taccccatgt aaattcattt ttacttatag gtataatgtc
     8461 agaacatttt actgaataac caagttgtgt tgaatacaat aattgaattg aattttcttt
     8521 gagttctaat gttttttcaa taaaatcaga ttttatactt gaagatatcc aagtagaatc
     8581 aatgctaaag aacacatctg tttcatattt aggttgatta gcttctaaag ctttaacatt
     8641 aaccaggaaa gtattatctt ttattgatgc ttctttggct atttttaatt tttctttaac
     8701 atatccactc aaataattat ctctatctac taaatatcct gtcaaacggt caatataagc
     8761 aagttgttta gataataatt catttttgat attatgatat gattgattca tgatgctaca
     8821 gatataatca atatttaaat atcctttgta ctatctaatt ttgagtaaaa taatgtaact
     8881 tctttttctt cattattttc cttagattta aattttttta aaacagtgtt tgttccagct
     8941 ggatatttta cttcaaatcc tagattgaat aaaaatctcc ttgttttaag aatattttgt
     9001 ttgttaattt cgttaaagtt tttaaatgtt tcacctaatt tttttgatag agtaaagata
     9061 aagtttttga tttcataaaa acctcctttt attttttatg ctaatagtta cagcttattt
     9121 ttatttgcta aagtatagct ttaatttaat ttgtatataa tatggggcat ttagaagtct
     9181 tttttgaaaa tttttataaa aaatcaaaaa aactgttatt aaaatgattt ataatcactt
     9241 aataacagtt ttaattatta acattatttt ttatagtaca aataagaatg tataatctaa
     9301 aaacgtttaa gaattagtat tttttagttc atatttccag gtttcaccac tttttatttt
     9361 ttctctctca aaataatttg gatatataac aataggaaaa ggaggcttat aaataggagt
     9421 aatattattt aaagtacaat aacggatgtt aaaatcttca tatcttagag tgttatcttt
     9481 tgtaatccag aaaaaatctt ctacactcat agcaagatta cttctacaaa tatcccaaac
     9541 gtcataatat cctagtttaa attcttttag tataaaagga acctctggtc tgcattcttg
     9601 aataactctt tcctcaatcc aaattcttat aaattcatcc catttatcac tatctaaatt
     9661 cttgaaaata tttgtgattg gaaatgggtt cattcccttg taatttttat tttgcttaaa
     9721 tgagaattta tcatttatta tattatatat taagattcct gccgtatcgt ttttatattt
     9781 taaaaaatat aagtcgttat ttctgattac tccattattt ttgatgatta gtttatcatc
     9841 aaaatttagc ttatcataaa ataaattatt catataaaac tcccctctct tttaaatatt
     9901 caattcttac ttttaacaag tgtttaatac attttaatct atgttgtctc aaaagatctt
     9961 tatatctatc aaattgtccc tagaaaatag tatttttata cccttatttg ttatacataa
    10021 ttttatttag aagataaact ttatataaaa caattaaaaa tatttctatt aagataaata
    10081 aaaaagagct atctcagaac aaatttaatt tgctcatgtg acacta
//
LOCUS       DAFNNB010000141         9997 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0144, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000141 DAFNNB010000000
VERSION     DAFNNB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9997
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0144"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1184
                     /gene="cls"
                     /locus_tag="L3M60_002899"
     CDS             <1..1184
                     /gene="cls"
                     /locus_tag="L3M60_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBN6227539.1"
                     /translation="LLRDEEDGSYRKRVINMLLKTGMFPFTKNNKVDVFVDGNEKFKR
                     LIEDIREAKDHIHLEYFIIKDSEIGRVLKEELIKKAKEGIKIRILYDDVGCWRFWFNR
                     KFFREMREVGIEIAAFLPTKFPIIGGKLNYRNHRKIVVIDGIIGYTGGINIGDEYLGK
                     NDKFGYWRDTHIRIKGISVYMLQMTFLIDWYYTTKEVLVTKNYFPSVGNIGESMIQVV
                     ASGPDSDWEDIHYAYFSAICQARKNVYIETPYFIPDESLLKAIKSAALSGVDVRIIFP
                     KIADHKIVNIASYSYFEEILRAGGKVYLYNKGFIHSKVVIIDDKIASAGTANMDLRSF
                     MLNFEVNAFIYDEEVIRVMTEDFFEDLSHCEELNLEVFKNRNIIQKIKESVARLFSPI
                     L"
     gene            1377..1661
                     /locus_tag="L3M60_002900"
     CDS             1377..1661
                     /locus_tag="L3M60_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBN6227540.1"
                     /translation="MKIRPLADRVVIKKVEAEEKTASGIVLPGAAKEQPQIAEVVEVG
                     PGGIVEGKEIKMELTVGDKVIFQKYSGTEVKIEGQEYTILRQSDVLAVIE"
     gene            1694..3322
                     /gene="groL"
                     /locus_tag="L3M60_002901"
     CDS             1694..3322
                     /gene="groL"
                     /locus_tag="L3M60_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729227.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBN6227541.1"
                     /translation="MAKEIKFSEETRRALEAGVNKLADTVKVTLGPKGRNVILDKKFG
                     SPLITNDGVTIAKEIELEDRFENMGAQLVKEVATKTNDVAGDGTTTATVLAQAIIREG
                     LKNVTAGANPILLRKGIQKAVTVAVEELKNQSRIVETQEAISQVASISAGDEEVGKLI
                     AEAMEIVGKDGVITVEESQTMNTELDAVEGMQFDRGFVSAYMVTDVDKMEAVLNDPYI
                     LITDKKISNIQELLPVLEQIVQQGKKLLIIAEDVEGEALSTLVVNKLRGTFDVVAVKA
                     PGFGDRRKEMLQDIAILTGAQVISEELGYDLKEADLSMLGRASSVKVTKESTTIVDGS
                     GDKKAIEERVTQIKHQVEQTTSDFDREKLMERLAKLAGGVAVIKVGAATEVELKERKL
                     RIEDALNATRAAVEEGIVAGGGTAFVSVIPAIGTLIESLEGEVKLGAQIVKKALEEPL
                     RQIAINAGLEGAVIVQNVVNSEAETGFDALNEKYVNMIEAGIVDPTKVSRSALQNAAS
                     IASTFLTTEAAVADLPEKEDAGMPGMGGGMPGMM"
     gene            3584..4324
                     /gene="larB"
                     /locus_tag="L3M60_002902"
     CDS             3584..4324
                     /gene="larB"
                     /locus_tag="L3M60_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HBN6227542.1"
                     /translation="MDLRKLLEEVKNENIDIDFALEKLKDLPYEDLGYANIDHHRELR
                     NGYPEVIYCEGKSDEHILGIIDKMNQKSSNILGTRCRKETFLKIKEIYSHAEYEDLSK
                     ILKIQNHDIENIGKGKIVIATGGTSDIPVADEAYHTAKFLGNDVDRIYDVGVAGIHRL
                     LNKRHKIDSARVIVAVAGMEGALASVIGGLVDVPVIAVPTSVGYGANFGGLAALLAML
                     NSCASGISVVNIDNGFGAGYLAAMINKL"
     gene            4408..5142
                     /gene="larC"
                     /locus_tag="L3M60_002903"
     CDS             4408..5142
                     /gene="larC"
                     /locus_tag="L3M60_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HBN6227543.1"
                     /translation="MSEKILYFDIISGISGDMTLASLLNLGVPKEIFLEEIHKLKMSD
                     EFNINISSKTENGIVGTKVEVITKEKHTHRNLVDIFEVIDESNLNENVKSKAKKIFMA
                     IGEAEAKVHGTTIDKIHFHEVGAIDSIVDIVGASILVDLLCVDKVCASTVSVGSGFVK
                     CEHGIIPIPAPATMEILKGVPIKLNNVNGECTTPTGAAIIKVLCDEFVDEFEFSPRQI
                     GYGIGHKKFEVPNMLRTVLGEKKKKN"
     gene            5172..5591
                     /locus_tag="L3M60_002904"
     CDS             5172..5591
                     /locus_tag="L3M60_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF111 family protein"
                     /protein_id="HBN6227544.1"
                     /translation="MSSEIYSYLYEKILGEKALDIYTESIYMKKNRPATKVSILCNES
                     DLEKFIKILLLETSTFGVRYNAYNREILERKFVKLDTKYGVVTVKLGYYKGELVKATP
                     EYNECKTIAKNEDIPIKKVFADINFRIKEEFSENLLT"
     regulatory      5651..5752
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            5973..7508
                     /gene="guaA"
                     /locus_tag="L3M60_002905"
     CDS             5973..7508
                     /gene="guaA"
                     /locus_tag="L3M60_002905"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBN6227545.1"
                     /translation="MKHELVLVIDFGGQYNQLIARRVRENNVYCEILPCTASIERIKE
                     KNPKGIIFTGGPNSAYLEDSPTISKEIFELGVPILGICYGIQIMSHVLGGVVRKGNKQ
                     EKEYGKTAITYGKSSLFEGITTNSVWMSHTDLIETVPEGFTIVANTNDCPVAAMENVE
                     RNLYGVQFHPEVEHCLEGDKILTNFLYNICKVKGDWTTDSFIEDKIKELKEKIGDKKA
                     LCALSGGVDSSVAAVLIHKAIGDNLTCIFVDHGLLRKNEGNDVERIFREKFDINLIRV
                     NAEDRFLSKLKGVSEPEAKRKIIGEEFIRVFEEESNKLGKMDFLVQGTIYPDVIESGH
                     GNAATIKSHHNVGGIPEDVDFQEIVEPLRELFKDEVRKIGLELGIEEGLIFRHPFPGP
                     GLGIRVIGDVTKEKCDILREADAVYMDELRKAGLYREIWQAFATLPDVKTVGVMGDER
                     TYAYLVGLRAVTSSDGMTSDWYKMPYDVLERISNRIINEVDGVNRVVYDITSKPPGTI
                     EWE"
     gene            7854..8264
                     /locus_tag="L3M60_002906"
     CDS             7854..8264
                     /locus_tag="L3M60_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227546.1"
                     /translation="MGKINSYNKEMESILIFDIGGISVKNTFSEVKLFQVKPDRLDDF
                     EKLIVLVAEEQKQQTGCIDIKYLKRFYIFDNIKEQPRELTKIVKCVKYYSYWEFDTLE
                     NYSNATHWFFDTYSKEVFKLLIMPFDINCGNSIY"
     gene            8408..8689
                     /locus_tag="L3M60_002907"
     CDS             8408..8689
                     /locus_tag="L3M60_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227547.1"
                     /translation="MLFIVIYILILFTIYLVKKNEFIGYILARGMLKLCFIIGIMLSI
                     QTVFQLVNKIEIPVPALITKVLIFVVLAVFARYIERNLKGNFIYSNSDF"
     gene            complement(8728..9300)
                     /locus_tag="L3M60_002908"
     CDS             complement(8728..9300)
                     /locus_tag="L3M60_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227548.1"
                     /translation="MINGDKISLIPATLDDRQKVYEWCFQSETTKSHSGLPNYPNIPI
                     ATYEEFCEEDYEEYFFTGAKPKDGRGFLIVSNTEPVGFISYSCFHLKQALAEIDIWMN
                     CEANCGKGFGVDAIISLGDFLNKTMDICELIIAPSRKNIRAVKAYKKAGFKPSTKPMS
                     DYLLDKYIPLYGTGDYGIDETVILVKQLDT"
     gene            9722..9964
                     /locus_tag="L3M60_002909"
                     /pseudo
     CDS             9722..9964
                     /locus_tag="L3M60_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892580.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
BASE COUNT         3903 a         1048 c         1876 g         3170 t
ORIGIN      
        1 agcttttaag agacgaagaa gatggtagtt atagaaaaag ggtaataaat atgctattaa
       61 agactggtat gtttcccttt actaaaaata ataaagtgga tgtgtttgta gatggaaatg
      121 aaaaatttaa aagacttata gaagatataa gagaagctaa agaccatatt catttagagt
      181 attttatcat caaagatagt gaaataggta gggtgttaaa agaggaactt ataaaaaaag
      241 ctaaagaagg tattaagata agaattttat atgatgatgt aggatgctgg agattttggt
      301 ttaatagaaa gtttttccga gaaatgaggg aagtaggtat agaaattgca gcatttttac
      361 ctacaaaatt tcctataata ggaggaaaac taaactatag aaatcacaga aaaattgttg
      421 ttattgatgg aataattgga tatacaggtg gaataaatat aggagatgaa tatttaggaa
      481 aaaatgataa gtttggatat tggagagata ctcatattag gattaagggt atttctgtat
      541 acatgcttca aatgacattt ctgattgatt ggtattatac gacaaaagaa gtattagtga
      601 ctaaaaatta ttttccaagt gtagggaata ttggtgaaag tatgatacag gttgttgcca
      661 gtggtccaga tagtgactgg gaagatattc attatgctta tttttctgca atatgtcaag
      721 ctagaaaaaa tgtttatata gaaactccat atttcattcc agatgaaagt ctattaaaag
      781 ctataaaaag tgctgctctt agtggagtag atgtgagaat aatatttcct aaaattgcag
      841 accacaagat agtaaacata gcatcttatt cgtattttga agaaatatta agagcaggtg
      901 gtaaagttta tttatataat aaaggattta tacactctaa agtcgtaata atagatgata
      961 aaattgcttc tgcaggtaca gccaatatgg acttaagaag ttttatgctt aactttgaag
     1021 taaatgcatt tatctatgat gaagaagtta ttagagtaat gacagaggat ttctttgagg
     1081 atttaagcca ctgtgaagaa cttaatttgg aggtatttaa gaatagaaat atcatacaaa
     1141 aaatcaaaga atctgtagca agattatttt caccaatatt ataaaaaatt attaaaaaag
     1201 gtattgaaaa ttatgtgtga ttggtatatt ataataattg ttagcactct attagataga
     1261 gtgataataa ataaaaagta tatatataaa tctacatata aaagtagtat taatttaaaa
     1321 ataactaaaa acataggcga actagtctat acttaatatt ttaggaggcg taaataatga
     1381 aaataagacc attagctgac agagtagtaa ttaaaaaagt agaagcagaa gaaaaaactg
     1441 caagtggaat agttttacca ggagcagcta aagagcaacc tcaaatagct gaagttgtag
     1501 aagttggacc aggtggaata gttgaaggga aagaaataaa aatggaattg acagtaggag
     1561 ataaggttat attccaaaaa tattctggaa cagaagttaa gatagaagga caagaataca
     1621 caatactaag acagagtgat gtattagctg ttattgaata aatatagaat aaatttatta
     1681 ggaggagttt aaaatggcta aagaaattaa attttcagag gaaacaagaa gagctttaga
     1741 agctggtgta aataaattag cagatacagt aaaagtaaca ttaggaccta aaggaagaaa
     1801 tgttatatta gataaaaaat ttggttctcc acttataact aatgatggag taactatagc
     1861 aaaagagata gagttagaag atagatttga aaatatgggt gctcaattag ttaaagaagt
     1921 agcaactaaa actaacgatg tagctggaga tggtactaca actgctacag ttttagcaca
     1981 agctataata agagaaggtt taaagaatgt aacagcaggg gctaacccaa tacttttaag
     2041 aaaaggaata caaaaggcag taacagtagc agtagaagaa ttaaaaaatc aatcaagaat
     2101 agtagaaaca caagaggcta tatctcaagt tgcttctata tctgctggag atgaagaggt
     2161 tggaaaatta atagcagaag ctatggaaat agtaggtaaa gatggagtta taactgttga
     2221 agagtctcaa actatgaata ctgaattaga tgctgttgaa ggtatgcaat ttgatagagg
     2281 atttgtttct gcatatatgg ttacagatgt agataaaatg gaagcagttt taaatgatcc
     2341 atatatatta attactgata aaaaaatatc taacatacaa gagttattac cagttcttga
     2401 gcaaatagtt caacaaggta aaaagttatt aataatagct gaagatgtag aaggtgaagc
     2461 gttatctaca ttagtagtaa ataaattaag aggaacattt gatgtagtag cagttaaagc
     2521 tccaggattt ggggatagaa gaaaagaaat gcttcaagat atagcaatac ttacaggtgc
     2581 tcaagtaata tcagaagaat taggctatga tttaaaagaa gctgatttat ctatgttagg
     2641 tagagcttca tctgttaagg taactaagga aagtactaca atagtagatg gttctggaga
     2701 taaaaaagct atagaagaga gagtaactca aattaaacat caagtagagc aaactacttc
     2761 agattttgat agagaaaaat taatggaaag attagctaaa cttgctggag gagtagctgt
     2821 aataaaagtt ggagctgcta cagaagtaga gttaaaagaa agaaagttaa gaatagaaga
     2881 tgctcttaat gcaactagag cagctgtaga agaaggaata gtagctggtg gaggaactgc
     2941 ttttgttagt gttataccag caataggaac gctaattgag agtttagaag gagaagttaa
     3001 actaggtgct caaatagtta aaaaagcgtt agaagaacca ttaagacaaa tagctataaa
     3061 tgctggtctt gaaggtgctg taattgtaca aaatgttgtt aattctgaag ctgaaactgg
     3121 atttgacgca ttaaatgaaa aatatgtaaa tatgatagaa gctggtatag ttgacccaac
     3181 taaagttagt agaagtgctt tacaaaatgc tgcatcaata gcaagtactt tcttaactac
     3241 tgaagcggct gttgctgacc ttcctgaaaa agaagacgca ggaatgccag gaatgggtgg
     3301 cggaatgcca ggtatgatgt aatttaaaaa atttaaaata taaattttaa tataaagaac
     3361 tatttcgact tagattttaa actctaagtt gaaatagttc ttttgtatat atctagtttt
     3421 tgtatattta taaattcaat acacatattt agataatcag tatctaggtt gtgataatat
     3481 cttagagtag tattgttatt ttttaggtta tgattttaga tatatattaa ttaaggtata
     3541 attgttgata gagactaaat caaatttaaa aggatgcgat aatatggact taagaaagtt
     3601 gcttgaagaa gttaaaaatg aaaatattga tatagatttt gcgttagaaa aattaaaaga
     3661 tttaccttat gaagaccttg gatatgcaaa tatagaccat catagagaat taagaaatgg
     3721 ttacccagaa gttatttatt gtgaaggtaa gagtgatgag catattttag ggattattga
     3781 taaaatgaat caaaaaagtt ctaatatatt ggggacaagg tgtagaaaag aaacttttct
     3841 aaaaattaaa gaaatctata gtcatgctga gtacgaagat ttgtctaaaa ttcttaagat
     3901 tcaaaatcat gatatagaga atataggaaa aggtaaaatt gtgattgcta cagggggaac
     3961 ttctgatata ccagtagcag acgaagccta tcatacagct aagtttttag ggaatgatgt
     4021 tgatagaata tatgatgttg gagtggcagg tatacatagg cttttaaata aaagacataa
     4081 aatagatagt gctagagtaa tagtagctgt tgcaggtatg gaaggtgctc ttgcaagtgt
     4141 aattggtgga ctagttgatg ttccagtaat agctgttcca acatctgttg gatatggagc
     4201 aaattttgga ggattagcag ccttactagc tatgttaaat agctgtgcat ctggaatatc
     4261 tgttgtaaat attgataatg gatttggagc aggatattta gcagcaatga taaataaact
     4321 ataaatacat tgatataaat taaacaaaac tatataataa aatttaattt ctattatatt
     4381 tttactatta aaattgagga gacatatatg agtgaaaaaa tactttattt tgatataata
     4441 agcggtatat caggggatat gacccttgca agtttattaa atttaggtgt gcctaaggaa
     4501 atttttttgg aggaaattca taaactaaaa atgagcgatg aatttaatat aaacatcagc
     4561 tctaaaactg aaaatggaat agtaggtaca aaagtagaag taattacaaa agaaaaacat
     4621 acacatagaa atttagtgga tatatttgag gttatagatg aaagtaatct aaacgaaaat
     4681 gtgaaatcta aagcaaaaaa aatatttatg gcaattggag aagcagaagc aaaagttcat
     4741 ggaactacaa tagacaagat acattttcat gaggtagggg ctatagattc aattgtagat
     4801 atagtaggag cttctatctt agttgattta ctttgtgtag ataaagtttg tgctagtaca
     4861 gtgtctgttg gctcaggatt tgtaaaatgt gaacatggaa ttatacctat acctgcacct
     4921 gcaactatgg aaattttaaa aggtgtaccg attaaattaa ataatgtaaa tggagaatgt
     4981 acaactccaa caggagctgc aatcataaaa gtcttgtgtg atgagtttgt agacgagttt
     5041 gaattttctc caagacaaat tggatatggt ataggtcata agaagtttga ggtaccaaat
     5101 atgctcagaa ctgtattagg tgaaaaaaaa aagaagaatt aatttatgaa attagtgcaa
     5161 acattgatga tatgtcttca gaaatctatt cttatttgta tgaaaagata ttaggtgaaa
     5221 aagcattaga tatttataca gagagcattt atatgaagaa gaataggcca gcaacaaaag
     5281 tatcaatttt atgcaatgaa tcagatttag aaaaatttat taaaatatta cttttagaaa
     5341 ctagtacttt tggagtgaga tataatgcat ataatagaga aattctagag agaaaatttg
     5401 tgaaattaga tacaaaatat ggagtggtta cagtaaaatt agggtattat aaaggagaac
     5461 tagtcaaggc gactccagaa tataatgaat gtaaaactat agcaaaaaat gaagatattc
     5521 ctataaaaaa agtgtttgct gatataaatt ttagaataaa agaagaattt agtgaaaact
     5581 tattgacata agtaataaat tttgataaat taatattcag ccttattaaa taaaagtaaa
     5641 aagaaaaaaa tatataaaat ataaactcac tcgtatatgc tcagaaatat ggtctgagag
     5701 tctctaccaa gataccgtaa atatcttgac tatgagtgaa attattatac caaaagacct
     5761 agttaagtaa atatgtaggc tgagttgtac tccctatgtg ttggctataa gataaaggta
     5821 ttttggaagc ctgtatagta atttcacttt tgctagaaat tatggaagtt actatacagg
     5881 ttttttgtgt aaaaagattt aatataattt agactgtatt gtgttaaaat aaatatataa
     5941 aaactatatt atacaaggag agatgtgaat ttatgaaaca tgaattagta cttgtaatag
     6001 attttggagg gcaatataat cagttgatag ctagaagagt tagagaaaac aacgtatatt
     6061 gtgaaatact tccatgtact gcaagcatag aaagaataaa agaaaaaaat cctaaaggga
     6121 taatatttac aggtggtcca aatagcgctt atttagaaga ttctccaaca atatcaaaag
     6181 aaatatttga actaggagta ccaatacttg gaatatgcta tggtattcaa ataatgtctc
     6241 atgtattggg tggagttgta agaaaaggca ataaacaaga aaaagaatat ggaaaaacag
     6301 ctataactta tggaaaatca tcattatttg aaggaataac tacaaatagt gtatggatga
     6361 gtcatacaga tttaatagaa acagttccag aaggatttac tatagttgca aataccaatg
     6421 attgtccagt agcagctatg gaaaatgtag aacgaaacct atatggagtt caattccatc
     6481 cagaggtaga acactgtttg gaaggggaca agatacttac aaacttctta tataatattt
     6541 gtaaagtaaa aggtgactgg actacagatt cgtttataga agataaaata aaagaattaa
     6601 aagaaaaaat aggcgataaa aaagctttat gtgctttaag tggaggagtt gattcttctg
     6661 ttgctgcagt tcttattcat aaagcgattg gggacaactt aacttgtata tttgttgacc
     6721 atggtttact tagaaaaaat gaaggaaatg atgtagaaag aatatttaga gagaaatttg
     6781 atataaattt aataagagtt aatgcagaag atagattctt atctaaatta aaaggagttt
     6841 ctgaaccaga agctaagaga aaaataatag gtgaagaatt tataagagta tttgaagagg
     6901 aatcaaataa attaggaaaa atggatttct tagtacaagg aacaatctat ccagatgtta
     6961 tagaaagtgg acatggaaat gctgctacta taaagtctca tcataatgtt ggtggaatcc
     7021 cagaagatgt agacttccaa gaaattgtag aaccattaag agagttattt aaggatgaag
     7081 ttagaaagat aggattagag cttggtatag aagaaggatt aatatttaga catcctttcc
     7141 caggaccagg tctagggata agagttatag gtgatgtaac taaagaaaaa tgtgatatat
     7201 taagagaagc tgatgctgtt tatatggatg agcttagaaa agcaggttta tatagagaaa
     7261 tatggcaagc ttttgcaaca ttaccagatg ttaagactgt tggagttatg ggtgatgaaa
     7321 gaacatatgc ttatctagtt ggtttaagag cggttacttc ttctgatgga atgacttctg
     7381 actggtataa gatgccttat gatgtattag aaagaatttc taatagaata ataaatgaag
     7441 ttgatggagt aaatagagta gtttatgata taacttctaa accacctgga actattgaat
     7501 gggaataaaa tatagaatga taaaaatccg tatgaacaca ggatttgctc tatcttctta
     7561 tcctatgcta tactagcttg atattattca tatttgcaag tatcttgtag aataataagt
     7621 aaaaagagca aaaatgctct gctgaacaat aaattcagca gagtattttt ttattttatt
     7681 ttgccgtgct cttgagctcc aagcattaga catagatgat tttgttagat tttggtttta
     7741 ttgtatcagc tctgaaaagt gaattgatac aataaaaaga ggcatttatt gattattgat
     7801 ggagatatat gttacgttac ttttgttgga aatttgagag atagcgtggt ataatgggta
     7861 aaataaattc atataacaaa gagatggaga gtatacttat atttgatata ggaggtatat
     7921 ctgtgaaaaa tactttttct gaggttaaat tgtttcaagt taagccagac aggctagatg
     7981 attttgaaaa attgatagtt cttgtagctg aagaacaaaa acagcaaaca ggttgtattg
     8041 acattaaata tttaaagagg ttctatattt ttgacaatat aaaggaacag cctagggaac
     8101 tgactaaaat tgttaaatgt gtaaaatatt attcttactg ggagtttgat actttagaaa
     8161 actattcaaa tgcaacacat tggtttttcg atacatattc aaaggaagta ttcaagcttt
     8221 taattatgcc ttttgacata aactgtggaa attcaattta ttgaattgaa tagcaaatga
     8281 gtagtatcac ctttggataa tggaaagtca aggtattttc ttatggattt tgtggagtta
     8341 agacactcga agttatgtac aggattcttt ttttaaaaaa cctattttta ttggtttaaa
     8401 tttgacaatg ctatttattg ttatctatat attgattttg tttacaatat atttagtcaa
     8461 gaaaaatgaa tttatagggt atattttagc tcgaggaatg ttaaagttgt gttttattat
     8521 aggtattatg ctttcaattc aaacggtatt tcaacttgtg aataagattg aaataccagt
     8581 tcctgcttta attactaaag tgttaatttt tgttgttttg gcggtatttg ctagatatat
     8641 tgaaagaaat ctaaaaggaa atttcattta tagcaacagt gatttttaga tgtagtaatg
     8701 aatgttgatg ttaaaaacaa cctctgacta agtatccaac tgttttacta aaataacagt
     8761 ttcatctatt ccataatctc cagtcccata gagaggtatg tatttatcca ataaataatc
     8821 actcattggt ttagtagatg gcttaaatcc agctttttta tatgccttga cagcccttat
     8881 attttttctt gatggtgcaa taattagttc gcaaatatcc attgttttat ttaaaaaatc
     8941 tccaagtgaa ataatggcat ctactccaaa accttttcca caattagcct cacagttcat
     9001 ccaaatgtca atttctgcaa gagcttgttt taaatgaaaa caagaatagc taataaatcc
     9061 aactggttct gtattactta ctatgagaaa acctctgcca tcttttggtt tagcaccagt
     9121 gaaaaaatac tcctcataat cctcttcaca aaattcttca taagttgcaa taggtatatt
     9181 tggataatta ggtagcccag aatgagattt tgtagtttca gactgaaaac accattcgta
     9241 tactttttgt ctatcatcta atgtggcagg aattaaactt attttatcac catttatcat
     9301 aatttttctc tccagtcttc acatagttta atatttactt acagtataag cttaaattga
     9361 taaattctaa cttacatata ttgtaacata tccaaaatac ataatcactg aatattgtta
     9421 tgtcgtaata gatttatgta atacagtgtt agaaaatatc acatatcaaa aacatcagaa
     9481 ttttactaga aatagtatat tttatttata tttaatatta ttcataattt tttatattcc
     9541 cctaattaaa aactctttgg tatacttata aataaactta atgatagaat aaaaaagctt
     9601 tataactatt agatgcatag aaaagcattt tatgtctatg aaatgtatat ctataaaaca
     9661 tatagatgac ttttatatat taatgtatta atattgatat tatagaagaa ttgagttgtc
     9721 aataatacta aatcaaatat ctatagaata tggagggatt atgaaaaata aaataattat
     9781 aggaattact ttttcacttg tattactaat gtctggatgt gtttcaaaac aaacagcaga
     9841 aataaacgta ttagcaacta ctgatttaca tggagttata ccttatgagt taacttctta
     9901 tgtaaaagaa gaaagaaaaa aagataaaaa tctaacttta gtagatgcag gagatttttt
     9961 tgattaaaaa atagctacat acactcgtaa ctttagt
//
LOCUS       DAFNNB010000142         9833 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0145, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000142 DAFNNB010000000
VERSION     DAFNNB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9833
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0145"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..438)
                     /locus_tag="L3M60_002910"
     CDS             complement(<1..438)
                     /locus_tag="L3M60_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HBN6227549.1"
                     /translation="MNTIIIYSSKYGCTKDCANILKNKLSDNITIVDINNNNKIELSK
                     FDKIIIGSSIYAGSVSKKIRLLCNDNLELLNKKQVGIFLCCGFSEEADKYLKSNFPSS
                     LLESANAIGIFGGEARLEEMKFLDKLIMKAVSKGNYDSFRISQD"
     gene            complement(441..845)
                     /locus_tag="L3M60_002911"
     CDS             complement(441..845)
                     /locus_tag="L3M60_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HBN6227550.1"
                     /translation="MKIHNKSSNKKKKYLKISITIFFVFICIIILIVSNLKPENLVVK
                     DINIANIKNGIYTGSADNKLVKATVSVEVNNGKIQNINILKHDNLLGKPAEKITTNII
                     KQQSLDVDAITSATYSSNTIRKAVENALRKGE"
     gene            complement(823..1449)
                     /locus_tag="L3M60_002912"
     CDS             complement(823..1449)
                     /locus_tag="L3M60_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227551.1"
                     /translation="MSIKERRKNEKEEMKKKIMDASIEIINQHGYENLSIRKIATKIE
                     YSPTTIYLYYKDKAEIISDMNNKLYSTVESNIIDIMNNCSSLPIDKQIIEIMTSFIKT
                     LSSEPEMAKAIMHSRMNTIFASENTNDTPSNNGIKMLDKCLSTGIEQGIFKKNIDKSS
                     WMLISALLGFVLCVVENKLYSLHNFSQLTENFLDILVGGLYNENSQQI"
     regulatory      1797..1840
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1941..2909
                     /locus_tag="L3M60_002913"
     CDS             1941..2909
                     /locus_tag="L3M60_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HBN6227552.1"
                     /translation="MEKRKVIIDCDPGIDDSLAILLALNSPELEVIGITTCCGNVPAN
                     IGAENALKTLQMCSSLNIPVYIGEEAPLKRKLVTAQDTHGEDGIGENFYQKVVGAKAK
                     NGAVDFIINTLHNHEKVSIIALAPLTNIAKALIKDRKAFENLDEFVSMGGAFRIHGNC
                     SPVAEFNYWVDPHGADYVYKNLSKKIHMVGLDVTRKIVLTPNTIEFINRLDKKMAKYI
                     TEITRFYIDFHWEQEGIIGCVINDPLAVAYFIDRSICKGFESYVEVVEDGIAMGQSIV
                     DSFNFYKKNPNAIVLNEVDEKKFMYMFLKRLFKGYEDLIDSVEGVI"
     gene            2911..3474
                     /locus_tag="L3M60_002914"
     CDS             2911..3474
                     /locus_tag="L3M60_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBN6227553.1"
                     /translation="MKRKICVKTITLIAFGISINIIGAFIAMGLRLPVYLDSIGTIMI
                     ASLLGPKYAVVTGVFGSLISGITFDLYSLYFAPVQISTGLLAGIAFKKNFLRGLKTPL
                     GVLLFAIPTSIISSIISAFLFGGMTSSGSSYIVQILKVLGLGDVFSVFVTQIFTDYAD
                     KLLAVVLVNLGLNAVPKTLKVSLTGGK"
     gene            3482..4171
                     /locus_tag="L3M60_002915"
     CDS             3482..4171
                     /locus_tag="L3M60_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011010721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBN6227554.1"
                     /translation="MDRYSEITNKNQREIVLLKGFPCIWGKCSFCDYIDDNSNLEEEM
                     NKLNLEVLKNVTGKYGVLEVINSGSCFELPKDTLEEIKLIIKEKNIKKLFLESHWSYR
                     NRLEEMREYFEIPVIFKIGVETFDNDFRNNILNKNANFKTPQDVKDYFDSPCIMVGIK
                     GQTKEMIDKDIEIILNTFEKATVNVFINNSSNIKRDEELVRWFVSKYKFLDENPNIEV
                     LYNNTDFGVGD"
     gene            4298..5026
                     /locus_tag="L3M60_002916"
     CDS             4298..5026
                     /locus_tag="L3M60_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HBN6227555.1"
                     /translation="MMFNRGKWNKLDYNELLEYMMSMQDIKHRDFNKKLIPGTENIIG
                     IRVPNLRKLSKEISKGNWQEFLEVAEDTYYEEVMLQGMIIGNINSNFEETLYYVKRFI
                     PKINNWSVCDGFCSGLKSMKKYKENMYDILKKYVYSENPWEIRFALVMFLIYYVDDKH
                     IKEIFEYCNNIQSEEYYVKMGMAWLLSICFIKYEEETFLYIKNNNLDDFTYNKTLQKI
                     IESNRVDLEKKNIIRSMKRKTRRY"
     gene            5040..5714
                     /locus_tag="L3M60_002917"
     CDS             5040..5714
                     /locus_tag="L3M60_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBN6227556.1"
                     /translation="MNNNEERIDKFLELQSKGFRFAEIAQNLKISQSTLRTFLNKKGY
                     KSEKGKYILKDELNYNKSLDNGDLEEEKVKEYEKSKKNTISNRKKVSTKKKVSNEKVL
                     SKSIEQIEFNDTGLKNAKPKKNVKPKKDRKINITQEDLDKLCEVYDWYLEVKDYNMKP
                     KKVSNKKDIHIDDETIDELKTTRIRIDKEVWNDFERLCSNSNFEKHQILTQALKEFMK
                     KYKNLL"
     gene            5740..6576
                     /locus_tag="L3M60_002918"
     CDS             5740..6576
                     /locus_tag="L3M60_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227557.1"
                     /translation="MNKIVKKSISLMIILTIFIFTLTACEKDEQPDSVDIQVEDKKEI
                     KIDEGNAKVLNIGKSEIINMDEGDKVDTSTKIENNSTFNISNVELIYNEYDENKKIIS
                     SDSKALLDMTLMPGKVAYIECGHKTFVKSVEVYAYEYEAEGKIVYVNLKENTIDIRNN
                     NIKLENSSQYEVLSTSELKKVNESNEEITYQVKVKNSSSKDLGNIILKTAEVNENGEY
                     LTVNRVPSYKILKPSEETDIDILCSTKAKSVEIVGYTYDDIKEKANVDIDLKSHKVKI
                     DK"
     gene            6592..7263
                     /locus_tag="L3M60_002919"
     CDS             6592..7263
                     /locus_tag="L3M60_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227558.1"
                     /translation="MKVLIVEDNKILLESVAEELRKHFETEKCEDGEEALYLVNQNIY
                     DLVILDLMLPNINGFDILKKMRVNNIDTPVLILTAKETLDDKVEAFTIGANDYLTKPF
                     YMEELVARVYAILRTNGKIKERNGLEFKSLYLDTLEKRVYIEKEEIKLQNKQFNLLEY
                     FVLNKGSILLKEQIYDRIWGIDSDATIEIVEVYVSNLRKKLSKYGYDKYIKTKRKVGY
                     IFDDK"
     gene            7253..8485
                     /locus_tag="L3M60_002920"
     CDS             7253..8485
                     /locus_tag="L3M60_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227559.1"
                     /translation="MINKNVFTSTKNHLIKMYIIVVGSFLIIFSIFIYSYFRGLTYSG
                     IDSEINDELEYIVSQFKRTSFLNPIRLKDPKDMVYVYEDGRISYYTQNEYFDELLPDR
                     RLDKKNSFFKYTENGYTFRELNVDAGRYQIQIIRNIDSEMNSLRQLTSVLIIGILISV
                     IITYFVAVYLTRKALIPIETAWKNQAKFIQDASHELRTPITIVSSKLESMLKSPESTV
                     NDEVETIATAMKETRRLKKMITDLLSLTKEDSIVKINLEEIDLEKLLEEISEDYIDIA
                     EFQEKRFVFNSKLKNKVIITDKNKLRQLILIFIDNAFKYTKLGDEIVLELKEDIEDEV
                     TLLISDTGIGIKKEEIPLIFDRFFRSENVRNKDLEGSGIGLSIARMISLNLSVEINVT
                     SDVDIGTTFELSIPKKLK"
     gene            8641..8958
                     /gene="trxA"
                     /locus_tag="L3M60_002921"
     CDS             8641..8958
                     /gene="trxA"
                     /locus_tag="L3M60_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HBN6227560.1"
                     /translation="MAKVINTSEFRSSVEGSKGVVVVDFFATWCGPCNMLGPVFAELG
                     EEMKDKARFVKVDIDESLEIAQQFNVSTVPTMIIFKDGKPVETLIGFMPKNKIEMQVK
                     SYL"
     gene            8975..>9833
                     /locus_tag="L3M60_002922"
     CDS             8975..>9833
                     /locus_tag="L3M60_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN6227561.1"
                     /translation="MRYDIAIIGSGPAGLSAAINAKIRNKTIIMFGNDNLSNKLVKAP
                     SVDNYLGFYDISGDELKDKFKSHIDSMDISIENKRINNIYAMGEYFTIMSGNDMYEAT
                     TVILATGVEYTRPIKGEEEFLGRGVGYCATCDAPLYRNKKVAVIGYNEESKEEANFLS
                     ELTSKTYFIPMYKKDNLMRSSDNLDDSIEVIHDRPVQIDGDKLVNKVSFKEKHIEVDG
                     VFVIKDSTSPSALVPGIEIDGIHIKVDNNMKTSIDGCFAAGDCVGKPYSYIKAAGQGQ
                     IAALNAVYYL"
BASE COUNT         3947 a          995 c         1631 g         3260 t
ORIGIN      
        1 atcctgtgat attctaaaac tgtcataatt accttttgaa acagctttca taataagttt
       61 atctaaaaat ttcatttctt ccaatcgagc ttcaccacca aaaattccaa tcgcatttgc
      121 actttctaac aaggatgatg gaaaatttga ttttaagtat ttatctgctt cttcagaaaa
      181 gccacagcaa aggaaaatac caacttgttt tttgtttaat aactctaaat tatcattaca
      241 tagtaatcgt attttttttg aaactgaacc agcatatata gagcttccaa taataatttt
      301 atcaaatttt gataattcaa ttttgttatt attattaata tcaacaatag tgatattatc
      361 tgagagttta ttttttagta tatttgcaca gtcttttgta caaccatatt tacttgaata
      421 tataataata gtattcataa ttattctcct tttcttaaag cattttctac agcttttcgt
      481 atagtattac tgctataagt tgcagaagtt atagcatcca catctaaaga ctgttgttta
      541 ataatatttg ttgtaatctt ttcagcaggt ttacctaaaa gattgtcgtg ttttaggatg
      601 ttaatatttt ggattttacc attattaact tcaacagaca cagtagcttt tactagttta
      661 ttatcagcac ttccagtata aatgccattt ttaatattag caatatttat atctttaact
      721 actaaatttt ctggttttaa gttgcttaca attaaaataa taatacatat aaatacaaag
      781 aatatagtaa tagatatttt gagatatttt ttctttttat tactagattt gttgtgaatt
      841 ttcattgtaa agtcctccca ctaaaatatc taagaaattt tctgtaagtt gagaaaaatt
      901 atgtaaagaa tataatttat tttcaacaac acataaaacg aatcctaaca atgcacttat
      961 aagcatccat gagcttttat ctatattttt tttaaatatt ccctgttcaa tcccagtgga
     1021 aaggcattta tcaagcattt ttataccatt attgctaggc gtatcatttg tattttcaga
     1081 tgcaaatata gtattcattc tactatgcat gatagctttt gccatctctg gttcactaga
     1141 aagagttttt ataaagctag tcataatttc tatgatttgt ttatctattg gtagcgacga
     1201 acaattattc ataatgtcaa taatattact ttctactgtg ctataaagtt tattgttcat
     1261 atcgctgata atctctgctt tgtctttgta gtaaagataa attgtagttg gagaatattc
     1321 tatttttgta gcaatttttc ttattgataa attttcatat ccatgttgat ttataatttc
     1381 gatgctagca tccataatct ttttcttcat ttcttctttt tcatttttac gtctttcttt
     1441 aatactcatt taatcacctc ttcacttaac agtgttaatt taatttacac tgttattgta
     1501 taatttaagt ttaatactgt caataaatta ttaaataata taagttaata tatttttatg
     1561 gctaataaat taatgttatt aattgtaata tttaagatat tattgtataa taataatcgg
     1621 tttaaatgcg ccacatttta ttaaagttgg ttaataattt taaataaact ttaataaaat
     1681 acattaaaat ataataaatt cttctatgct ataaaattta ttttatagct attaaatcgg
     1741 atgtttaaaa attttttaag agtctatagt aaggaatcta aaattataat tttacatctg
     1801 tacttgttcg agaactccaa gtataaaaaa ctaaggaatt atatagtggc agtatataat
     1861 taataccctt agacacacct ttttgaaagt ggaaaaaaga tgtgtagttc tcctcgacca
     1921 caaatcaagg aggataaaaa atggaaaaaa gaaaagtaat aatcgattgt gacccaggaa
     1981 ttgatgattc tttggcaatt cttctggctt taaactcacc agagctagaa gtaattggaa
     2041 ttaccacgtg ctgtggaaat gttccagcaa atataggtgc agaaaatgca ctaaaaacac
     2101 ttcaaatgtg ttcttcacta aatattccag tatatatagg agaagaagca ccactaaaaa
     2161 gaaaacttgt aacagctcaa gatacacatg gagaagatgg tattggagaa aacttttatc
     2221 aaaaagttgt aggagctaaa gcaaaaaatg gagcagtgga ttttataata aatactttac
     2281 ataatcatga aaaagtatca ataatagcac ttgcaccact tacaaacata gctaaagcac
     2341 ttattaaaga taggaaagca tttgaaaatc tcgatgagtt tgtatctatg ggaggagcat
     2401 ttaggattca tggaaattgc tctccagtag cagagtttaa ctattgggta gacccacatg
     2461 gagcagatta tgtttacaag aatttatcta aaaaaatcca tatggtaggt ttagatgtaa
     2521 ccagaaaaat tgtacttact cctaatacta ttgagtttat aaatagactt gataagaaaa
     2581 tggcaaagta tataactgaa ataactagat tctatattga tttccattgg gaacaggagg
     2641 gaataattgg ctgtgtgata aatgaccctt tagcagtagc gtattttata gatagaagta
     2701 tatgtaaagg atttgaatca tatgtagaag ttgtagaaga cgggatagct atgggtcagt
     2761 ctatagtaga ttctttcaat ttctataaaa aaaatcctaa tgcaattgtt ctaaatgaag
     2821 ttgatgagaa gaagtttatg tacatgtttt taaagaggct ttttaaaggt tatgaagacc
     2881 ttatagactc tgtggaagga gtgatatagt gtgaaaagaa aaatatgtgt aaaaactatt
     2941 acacttatag cttttggtat ttccataaat ataataggcg cttttatagc tatgggatta
     3001 agattacctg tatatttaga ttcaatagga actataatga tagcttcact tttaggacct
     3061 aaatacgctg ttgttacagg tgtgtttgga agtttaataa gtggaattac gtttgatttg
     3121 tattctctat attttgcacc agttcaaata tcaacaggat tacttgctgg aatagcattt
     3181 aaaaagaatt ttttaagggg tttaaaaact ccactaggag tactcttatt tgccattcca
     3241 acgtctataa taagttcaat aatttcagca tttttatttg gaggtatgac atcttcgggt
     3301 tcatcctata tagtacaaat cttgaaagta ttaggattgg gagatgtttt tagtgtgttt
     3361 gtaacccaaa tatttactga ttatgcagat aagttgttag cagttgtttt agtgaattta
     3421 ggactaaatg cagttccaaa aactttaaag gtgtcattga ctggaggaaa atagatataa
     3481 aatggataga tatagtgaaa taacaaataa aaatcaaaga gaaattgttc tgcttaaagg
     3541 atttccttgt atatggggaa agtgttcttt ttgtgattat atagatgata attctaattt
     3601 agaagaagag atgaataaat taaatctaga ggttttaaaa aatgtaactg gaaaatatgg
     3661 agttcttgag gtcataaatt ctggaagttg ttttgaatta cctaaggata ctttagaaga
     3721 aataaagctt attataaaag agaagaatat aaaaaaatta tttttagaaa gtcattggtc
     3781 ttacagaaat aggcttgagg aaatgagaga gtattttgaa attcctgtga tatttaaaat
     3841 tggagttgaa acctttgaca atgactttag aaataatatt ttaaataaaa atgcaaattt
     3901 taagactcca caagatgtaa aagactattt tgattcacct tgtattatgg ttggaataaa
     3961 aggacaaaca aaagagatga tagataaaga tatagagatt atcttaaata cttttgaaaa
     4021 agcgacagtg aatgttttta taaataattc ttctaatata aaaagagatg aagagttagt
     4081 gagatggttt gttagtaagt ataaattctt ggatgaaaat ccaaatatag aagttttata
     4141 caataataca gactttggtg taggagatta gcattaattt agtttaaatt taaaattgaa
     4201 aaatttctta tgtttttgaa aaagcttcta atagttagaa gctttttttg tagagtttta
     4261 tgttacaata agaaaaaagg aattaaggag caaataaatg atgtttaata ggggaaaatg
     4321 gaataaattg gattataatg aacttttaga atatatgatg tctatgcaag atataaaaca
     4381 tagagatttt aataaaaagc ttatacctgg aacagaaaat ataataggta ttagagttcc
     4441 taatttgaga aagttatcaa aagaaataag caaagggaat tggcaggaat ttttagaagt
     4501 agcagaggat acatactatg aagaagttat gctacaaggg atgataattg gcaatataaa
     4561 tagtaatttt gaagaaactt tatactatgt aaaaagattt atacctaaaa ttaataattg
     4621 gtcagtttgt gatggatttt gttctggttt aaagtctatg aagaaatata aagaaaacat
     4681 gtatgatata cttaaaaaat atgtttattc agagaatcca tgggaaatta gatttgcatt
     4741 agttatgttt ttaatatatt atgttgatga taaacatatt aaagaaatat ttgaatactg
     4801 taataatata caaagtgaag aatattatgt taaaatgggt atggcatggt tgctatcaat
     4861 ttgttttatt aaatatgaag aagaaacttt tctttatata aaaaataaca atttagatga
     4921 ttttacttat aataaaacgc tacaaaaaat aattgaatca aatagagtag atttagaaaa
     4981 gaagaatata ataagaagta tgaaaagaaa gacaagaaga tattgacgga ggaatattaa
     5041 tgaataacaa tgaagaaaga attgacaaat ttttagaact tcaaagcaaa ggctttagat
     5101 ttgcagagat agctcaaaat ctaaagatta gtcaaagtac tttaagaact tttttaaata
     5161 aaaaagggta taagtcagaa aaaggtaaat acatattaaa agacgaatta aattataata
     5221 aaagtttaga taatggagac ttggaagaag aaaaagttaa agagtatgaa aaaagtaaaa
     5281 agaatactat aagtaataga aaaaaagtaa gcaccaaaaa aaaggtttct aatgaaaagg
     5341 ttttaagtaa aagtattgaa caaattgagt ttaatgatac aggcttaaaa aatgcaaagc
     5401 caaaaaaaaa tgttaagcct aagaaagata gaaaaataaa tataactcaa gaagatttag
     5461 ataagctttg tgaagtatat gattggtact tagaagtaaa agattacaat atgaagccta
     5521 aaaaagtatc taataaaaaa gatatacata tagatgatga aaccatagat gaattaaaaa
     5581 caacaagaat acgtatagat aaggaagtat ggaatgattt tgaaagatta tgtagtaatt
     5641 caaattttga aaaacatcaa atacttacac aagcattgaa agaatttatg aaaaaatata
     5701 aaaatctact ataaaatact actatatgag gtgcttaata tgaataaaat agtaaaaaaa
     5761 tcaatttctt taatgattat attaacaata tttattttta cgttgacagc atgtgaaaaa
     5821 gatgaacaac ctgattcagt tgatattcaa gtagaagata aaaaagagat aaaaatagat
     5881 gagggaaatg caaaagtatt gaatatagga aaatcagaaa ttataaatat ggatgaaggt
     5941 gataaagtag atacatccac aaagatagaa aataactcaa catttaatat atccaatgtt
     6001 gaattgatat ataatgaata tgatgagaat aaaaaaatta tatcttcaga tagtaaggct
     6061 ttgttggaca tgactttaat gcctggaaaa gttgcttata tagaatgtgg gcataaaacc
     6121 tttgtaaaga gtgtagaagt ttatgcatat gaatatgagg cagagggtaa aattgtgtat
     6181 gttaatttaa aagaaaatac tattgatata agaaataata atattaagct agaaaatagt
     6241 agtcagtatg aggtactatc tacatcagaa cttaaaaaag taaatgaatc aaatgaggaa
     6301 attacgtatc aagtaaaagt aaaaaattca tcatcaaaag atttaggaaa tattattctt
     6361 aagacagcag aagttaatga aaatggagaa tatttaacag taaatcgtgt accatcatat
     6421 aagattttga agccttcaga agagacagat atagatatat tgtgctccac aaaggcaaaa
     6481 agtgttgaaa tagtaggata tacatatgat gatataaagg aaaaagccaa tgtggatatt
     6541 gacttaaaat ctcataaagt taagatagac aaataaaggt aggggatact tatgaaggtt
     6601 ttaatagttg aagacaataa gatacttcta gaaagtgtag cagaagagct tagaaagcat
     6661 tttgaaactg aaaagtgtga agatggagaa gaagctttat acttagtaaa tcaaaatata
     6721 tatgatttag ttattttaga cttaatgctt cctaatataa atggatttga tatcctaaag
     6781 aaaatgagag ttaataatat agatacacct gtacttatat tgacagcaaa agaaacatta
     6841 gatgacaaag tggaagcatt tactatagga gctaatgatt atttaactaa acctttttat
     6901 atggaagagt tagtagcaag ggtgtatgct atattaagga ctaatggaaa gataaaagaa
     6961 agaaacggat tagaatttaa atctttatat ttagatacac tagaaaaaag agtgtatatt
     7021 gaaaaagaag aaataaaact tcaaaacaaa cagtttaact tgcttgagta ttttgtacta
     7081 aataaaggtt caattttatt aaaagagcag atttatgata gaatatgggg aattgattca
     7141 gatgcaacta ttgaaatagt ggaagtatat gtaagtaatc taagaaaaaa gctcagtaaa
     7201 tatggatatg ataaatatat taaaactaaa cgtaaggtag gatatatatt cgatgataaa
     7261 taagaatgta tttactagta ctaaaaatca tctaataaaa atgtatatta ttgtagtagg
     7321 tagtttttta ataatctttt ctatatttat atattcatat tttaggggac ttacatatag
     7381 tggaattgat agtgagataa atgatgagct tgaatatata gtttctcaat ttaaaagaac
     7441 ttcatttttg aacccaataa gactcaaaga ccctaaagat atggtgtatg tatatgaaga
     7501 tggaaggata agttactata ctcaaaatga atattttgat gagttgcttc cagatagaag
     7561 actagataaa aaaaattcgt tttttaaata cacagaaaat ggatatactt tcagagaatt
     7621 aaatgtagat gcaggtagat accaaataca gataattaga aatatagatt ctgagatgaa
     7681 ttctttaaga cagcttacat ctgttcttat aattggaata ttaatttctg taataattac
     7741 ttactttgta gctgtatatc tcactagaaa agctttaatt ccaatagaaa cagcatggaa
     7801 aaatcaagct aagtttatac aagatgcttc tcatgaatta agaacaccaa taacaatagt
     7861 ttcatcaaaa ttagaaagta tgttaaaatc cccagaaagt acagtaaatg atgaagttga
     7921 aacaatagct actgcaatga aggaaactag gagacttaaa aaaatgataa ctgatttatt
     7981 gtctcttact aaagaggatt ctattgttaa aattaattta gaagaaatag acttagaaaa
     8041 gttattggaa gaaatatcag aagactatat tgacatagct gaatttcagg aaaaaagatt
     8101 cgtatttaat tctaaactga agaataaagt tataataaca gataaaaata aattaagaca
     8161 gcttatattg atttttattg ataatgcctt taaatataca aaattgggag atgagatagt
     8221 attagaatta aaagaagata tagaagatga ggtaactttg cttataagtg atactggtat
     8281 aggtataaaa aaagaagaaa tacctcttat atttgataga ttttttagaa gtgaaaatgt
     8341 aagaaataaa gatttagaag gttctggaat aggactttca atagctcgaa tgattagctt
     8401 aaacttgtct gttgagataa atgtaacttc tgatgtagat attggaacta catttgaatt
     8461 atcaatacca aaaaagttaa aataataaaa atataaaaag ttgtttttag aaataaagaa
     8521 tgggtatact aattagtata aaataaaaaa taaaaagacc ttataaaagt aaaattatat
     8581 ttataaattt catagtataa ttatataggg ataataaagt ttaataggag gatttaaaaa
     8641 atggcaaaag ttataaatac atctgaattt agaagtagtg ttgaaggtag taagggagta
     8701 gttgtagttg atttctttgc aacttggtgt ggaccttgta acatgttggg acctgtattt
     8761 gcagaattag gtgaagagat gaaagataaa gctagatttg taaaagttga tatagatgag
     8821 agcttagaaa tagctcaaca attcaatgtg tctacagtac caactatgat tatatttaaa
     8881 gatggaaaac cagtagaaac tttaattgga ttcatgccaa agaacaaaat tgaaatgcaa
     8941 gtaaaatcat atttataaaa aatgggtgag tattatgaga tatgatatag ctataattgg
     9001 tagtggtcca gctggattgt ctgctgctat aaatgcaaaa ataagaaata aaacaattat
     9061 catgtttggt aatgataatt taagtaataa gcttgttaaa gccccttcag tagataatta
     9121 tttaggtttt tatgatataa gtggtgatga attaaaagat aagtttaaat cacatataga
     9181 tagtatggat atttcaattg aaaataaaag aataaataat atttatgcta tgggagaata
     9241 ctttacaata atgtctggaa atgatatgta tgaggctact actgtaatcc ttgcaactgg
     9301 agtagagtat actagaccaa tcaagggtga agaagaattt ctaggaagag gtgtaggcta
     9361 ttgtgctaca tgtgatgcac cactttatag aaataaaaaa gtagcagtta taggatataa
     9421 tgaagaatct aaagaagaag caaatttttt aagtgaactc acttcaaaga cttattttat
     9481 acctatgtac aaaaaagata atcttatgag aagttcagat aacctagatg attctattga
     9541 agtcatacat gacagaccag tacaaataga tggagataaa cttgtaaaca aagtatcttt
     9601 taaagaaaaa cacattgaag ttgatggagt attcgttata aaagatagta catctccaag
     9661 tgccttggtt cctggaatag aaattgatgg cattcatatt aaagttgaca ataatatgaa
     9721 aactagtata gatggatgtt ttgctgctgg agattgtgtt ggaaaaccat attcttatat
     9781 aaaagcagct ggccaagggc aaatagcagc tttaaatgct gtttactatt tag
//
LOCUS       DAFNNB010000143         9740 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0147, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000143 DAFNNB010000000
VERSION     DAFNNB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0147"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            69..263
                     /locus_tag="L3M60_002923"
     CDS             69..263
                     /locus_tag="L3M60_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HBN6227562.1"
                     /translation="MSVIVMCSRCGKEIDKYRNKYITYYEKNTNAEIKVCINCALDLL
                     EKTRENQELNIQEFQDTIIL"
     gene            complement(372..881)
                     /gene="vanZ1"
                     /locus_tag="L3M60_002924"
     CDS             complement(372..881)
                     /gene="vanZ1"
                     /locus_tag="L3M60_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ1"
                     /protein_id="HBN6227563.1"
                     /translation="MKSKKYNITKGLFIVYIIILTWIILFKLQFDVSSIETMNLRSIN
                     LVPFAGSLIINNKVDISEIILNVAIFIPFGIYICMLKEEWSFIKKVIPIFITSLAFET
                     LQYIFALGASDITDLIGNTLGGIIGIAVFMLLSKIFKNNTIKIINILAFIVTIIVVLF
                     LGLVIFANL"
     gene            1417..1581
                     /locus_tag="L3M60_002925"
     CDS             1417..1581
                     /locus_tag="L3M60_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBN6227564.1"
                     /translation="MFFHSTYNNGSYISHIGIYIGNNHMYHVNNPIGYADLTSAYLQQ
                     HLIGAGRIKQ"
     gene            1905..2342
                     /locus_tag="L3M60_002926"
     CDS             1905..2342
                     /locus_tag="L3M60_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN6227565.1"
                     /translation="MEEHEIFNTFVQILNWASERNKNSKSYGTDDKLYIAEIHTLVMI
                     GKMPGILQKELCKEVGVTKGRMSVIIAHLQEKNLIEMRREQGNGCTIPIYLTTLGEQI
                     YHNHNEQEMNMKIQIYQVLSKCSQEEIEKFNGILRDILEILRQ"
     gene            2398..3057
                     /locus_tag="L3M60_002927"
     CDS             2398..3057
                     /locus_tag="L3M60_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HBN6227566.1"
                     /translation="MNIVVGVLSILSAFCIAGSMIGLMFSFGYPKIIRENPIIILKRM
                     QNKKTMISLLFYLFGIGGFMVVQASIALRFIEQSKGEFIWSNFAYINGIIYGVLLFVG
                     ILRYTKTFPMLSNQMINKQITEKEAGQLYKVLNTYIGETMTEHVAFVFLSFMIFCNSM
                     SLLSINYVANWIGLLGIIIAIGLFIGNLEFLGIKKLFIVNRVFSSLSAFWLLLLGICI
                     I"
     gene            3515..3703
                     /locus_tag="L3M60_002928"
     CDS             3515..3703
                     /locus_tag="L3M60_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227567.1"
                     /translation="MIGFKKRDTKYLLKIVARAHGISVAEAIVEMQTTINNARNNPDP
                     EKQAEFIISLNTIFTKNL"
     gene            complement(3868..4119)
                     /locus_tag="L3M60_002929"
     CDS             complement(3868..4119)
                     /locus_tag="L3M60_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227568.1"
                     /translation="MSSYFITRGDVMKILQQFKPYKYDKEKNIEYDEKIIANQNTEVY
                     IVYPRISDEENRRRWEEFNEVAKEVAINIAMKKVETVES"
     gene            4298..4432
                     /locus_tag="L3M60_002930"
     CDS             4298..4432
                     /locus_tag="L3M60_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227569.1"
                     /translation="MFEKTNINTSVMNIIESDERPIRDDELIIFAKIFGVSINYILGL
                     "
     repeat_region   4510..5136
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4510..4538
                     /rpt_unit_seq="ctttacataccacttagttaatataaaac"
     gene            complement(5423..5581)
                     /locus_tag="L3M60_002931"
     CDS             complement(5423..5581)
                     /locus_tag="L3M60_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227570.1"
                     /translation="MDNFLLSILASILASYIVYLISKLFRKRKKPLKAGTKSGWELDF
                     KIKFHKFK"
     gene            complement(5719..7164)
                     /locus_tag="L3M60_002932"
     CDS             complement(5719..7164)
                     /locus_tag="L3M60_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6227571.1"
                     /translation="MKIKKLLTIGLSLSMFIASCHISANALDKIESIKGADKYETTGI
                     IADKQNYTTAILINADSTMADGLSASGLAGATNAPILLTKKNNIPNATLKRLEKAKKV
                     YIIGGENSIDKYTETVLKGKGIEIKRLQGSDRIKTSYNVAKEINSINKVNKVILTNAF
                     KGEPDAMSAAPVAVREKAAIVLTDGKSVPFNTTGVESYVIGGTSSMNDKLVNDTNSTR
                     LGGTDRYDTNKKIINKFYGGVKEFYIASGTDLVYALVGSTVAKNNAIVLVNNDSNKSV
                     LKSTTKLTAIGNLSDSILQQCLNVTKNIGDSNTGRQYTVEDAANAVKKLLGVKKFYYN
                     ENYPEDEDVIFLLDENFEFSGKQFYAFGYVTENSEGDVRYCVEKNDMSKVYTYNVSGV
                     MQVCNPDDYKKKEMISEEKAKQLALEECARKHKVGVDLLKVNIAYIDDFDDIKVYAVR
                     IDSKPHPNQTYGWYFIDVYSGYAYKDVWNNI"
     gene            complement(7204..8016)
                     /locus_tag="L3M60_002933"
     CDS             complement(7204..8016)
                     /locus_tag="L3M60_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227572.1"
                     /translation="MCNFKKILGLFMIIMLSVSMVACSSGGDSKDESKGKDKQFIEDL
                     GKGLKKRWDLASKEEAGTLNLEQNEAYEKFVEVEMDAVSKYKDAEFDSPELGKLAKDY
                     IKALEDSKEAIKYYSNDQLKFSELWEKPYNERSTILLTLVDKFGLKVDEKEFKVLKDN
                     AQIVNEENDLKEKVNAMAKGIKFEKVKDEYDWKTYQATVENTSDLDLQDFYLDINLID
                     KDGVIIETQTSTSVNGTWKKGQKVRLEFSTDKDFEKIEWTPHYSANNYSITE"
     gene            8198..9712
                     /locus_tag="L3M60_002934"
     CDS             8198..9712
                     /locus_tag="L3M60_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBN6227573.1"
                     /translation="MSVAIYLRKSRADEEAEKQGEFETLSRHKSTLLKLAKEQSLDVI
                     EIKEELVSGESIIHRPKMLELLKEVEENKYDAVLVMDLDRLGRGDMKDQGIILETFKE
                     SKTKIITPRKTYDLTDEFDEEYSEFEAFMARKELKLISRRMQRGRIKSVEEGNFIGTS
                     APFGYDAVTTGRKERILVPNKDADIVRTIFDLYINEDMGCSKISKYLNNLGIKTATGA
                     NWYNSAITNIIKNKVYCGYIQWQKKDYKKSKNPNKIKTVKLRPKDEWIEAKGKHEPLI
                     SEITWKKAQNILKKNGHVSYGNQIKNPLAGIVICKNCGRPLVYRPYADHNYIICYHPG
                     CNKSSRFEFIEAAILKSLEDTVKKYQLKTSDIDLDKNNKDSNIEFQKRVLKGLETELK
                     ELSKQKNKLYDLLERGIYDEDTFIERSNNISSRTEEIKDSIKTVKNRLSTVKKDNAKI
                     IEDIKTVLSLYHDSDSLGKNKLLKSVIDKAVYYKSKEQKLDSFELMVHLKLHED"
BASE COUNT         3535 a         1469 c         1249 g         3487 t
ORIGIN      
        1 atcattattt tcagaaaagt gatactattg aatatattaa gaataattag agatggaggt
       61 actaagctat gagcgtaatt gtaatgtgta gtcgttgtgg aaaagaaata gataaatata
      121 gaaacaaata tataacttat tatgaaaaaa ataccaatgc agaaattaaa gtttgtataa
      181 attgtgcgtt agatttatta gaaaaaacaa gagaaaatca agaattgaat atacaagagt
      241 ttcaagatac aataattctt taataattta agaagcactt atatgtctat ataggtgctt
      301 cttatgtgta aataagttta tatgtaactg tattttacgc tctattttaa gttaaaccta
      361 tatacttatt tctataagtt tgcaaaaata actaagccta aaaataatac aacaataatt
      421 gtcacaataa atgctaatat attaataatt tttatggtat tattcttaaa tattttcgac
      481 aatagcataa aaacagctat tcctatgatt cctcctagtg tatttcctat caaatcggtt
      541 atatcacttg cacctagtgc aaatatatat tgaagtgttt caaatgccaa actagttata
      601 aaaataggta taactttttt tataaagctc cactcttctt ttagcataca tatgtatatc
      661 ccaaatggta taaatatagc cacatttaat ataatctctg atatatctac tttattatta
      721 attataagtg acccagcaaa aggtactaag tttatgcttc taagattcat ggtttcaatg
      781 gatgaaacat caaattgtaa cttgaatagt attatccatg tcaaaataat tatgtaaact
      841 ataaataatc cttttgtaat attgtatttt ttagatttca ttcattacct ccttactata
      901 ttattgtatt aatgatttaa tacaataata tagtaaggca atttatctgt caactatttc
      961 ttctaaaatt atatagcttt tataatttca atttatttat catatttgca tgatttaaac
     1021 tattgtagta tttcatatat ttctttaaat tttaatcggt attattttct aaatttttta
     1081 ctatcaaata caaggaaata agcacttttc tttatataat tttctaccat tacataacac
     1141 tagcattaaa tcaaaacact attctcaatt ttctcaaact aaaaagatac tgatagcttt
     1201 attagtcatc aatacatttt tcttgaaaat ttttaaaata ttcttctaat ttgtccaagc
     1261 ttttactatc taaaacgtca tcatcatgta gtgctgaaat aaaaccctta aatgaattgt
     1321 tatgaataaa actaaagaaa ctatttttct caacttttaa ataatctttc tcttttatag
     1381 ctcatcccgc ttttacaggc aaaagctgac aacctcatgt ttttccactc tacttataac
     1441 aacggtagct atataagcca catcggaata tacattggca acaaccatat gtaccatgtg
     1501 aacaatccga ttggctatgc agaccttacg agtgcctact tgcaacagca cctaataggt
     1561 gcaggacgta tcaaacaata gaaaggaatt gaaatcatgt tcaagaaaaa agagaacgag
     1621 aaaaaagtaa taaaacagaa aaactcacga acaatgaaaa tcggcttgtc agtgaagttt
     1681 attgataatc agacaaaggt gacacagatt tcacagtttg agttgaggat tcacaaagat
     1741 agcaactgga agatgttaaa gtaagataaa tataaaacct tacttctaca tctaaatagc
     1801 agtaaccctt atttacatgt tttttataat aatagaatgt tctatcaaat atgacttgac
     1861 ctcttacagt ttacggggta aactgtattt agaggtgaac aaaaatggaa gaacatgaga
     1921 tatttaatac ttttgtacag attttaaatt gggcttctga acgtaataaa aattcaaagt
     1981 cctatggaac tgatgataaa ttatatattg cagagattca tacacttgta atgattggaa
     2041 aaatgccagg tattctgcaa aaagaactat gtaaagaagt aggtgtaaca aaaggaagaa
     2101 tgtccgtcat tattgcacat ttacaagaaa aaaatttaat tgaaatgcga cgagaacaag
     2161 gtaacggatg tacaattcct atctatttaa ctactcttgg agaacaaatt tatcataatc
     2221 acaatgagca ggaaatgaat atgaaaattc agatttatca agttttatca aaatgtagtc
     2281 aagaagagat agaaaagttc aacggcatat tgagagatat tctagaaatt ttaagacaat
     2341 aagttagttt tattactttg atatatttaa aaaatataat ttagaaaaga ggaaaatatg
     2401 aatatagttg taggtgtttt atcaatatta tcagcttttt gtattgctgg aagtatgata
     2461 ggtttaatgt ttagttttgg ttatcctaaa attattagag aaaatcctat tataattcta
     2521 aagagaatgc agaataagaa aactatgatt tctttgctat tctatctatt tggaattggt
     2581 ggttttatgg ttgtccaggc atctattgca ttacgtttta ttgaacaaag taagggagaa
     2641 tttatttggt caaactttgc atatatcaat ggaataatct acggagttct tctattcgta
     2701 ggaatcttga gatatacaaa gacctttcca atgttgtcta accagatgat aaataaacag
     2761 attactgaaa aagaagctgg acaattatac aaagtattaa acacatatat aggtgaaacc
     2821 atgacagagc atgttgcatt tgtattttta agttttatga ttttctgtaa ttcaatgagt
     2881 ttactgtcta taaattatgt agcaaattgg attgggcttt taggtataat catagcaatt
     2941 ggtcttttta tcggcaactt agaattttta ggtattaaaa aattgttcat agttaatagg
     3001 gttttttcat cattaagtgc tttctggcta ttgttattag gaatatgtat tatataaaga
     3061 ttatgttact taagaatatt tagatttgtt tgctataaag taggtgggaa aaaggggaaa
     3121 ttatatatat aactgctaaa gaatttgatt tgatgtattt tctcttttgc cataaaggaa
     3181 tggtattcac gaaataacaa ctatatgata acatatggag cttcaataat gcgccagagg
     3241 gtagcaatct gacatcattt atacgaaagt taagaaataa gatagaaaat agagccgtcc
     3301 tcagataatc tacagtacat attgactgtt tggggtgttg gctataaatt caacgaagaa
     3361 aaaccgtaac cgtatttcac attaaacatg tgattaacag gatacggttt tattcattct
     3421 catttattat agtttctctt ataagggtgt cataaaataa atcacaccat gagttatggt
     3481 tgcaaatggt caaattaaga aatgagtcat atatatgatt ggatttaaga aacgagatac
     3541 aaaatacctg ttaaagattg ttgcccgtgc acatggcatt tctgttgcag aagccatagt
     3601 agaaatgcag acaacgataa acaatgcaag gaacaatcca gaccccgaaa aacaggcaga
     3661 gtttataata tccttaaata ctatatttac taaaaactta tagtttcttt taatagttaa
     3721 tacattctta ttactaataa caatactcaa caattttatc accttttttc aaatatgatt
     3781 taactttttt attcctagtt atttaaaaat agttgtccaa ttttcttcta tgagaaagtg
     3841 ctaagtatta gcaacttctc tattctctta actttctact gtctcaactt tcttcatagc
     3901 tatattaatg gctacttctt tagctacttc attgaactct tcccatcttc tcctattttc
     3961 ttcatcagat atacgtggat atactatata aacttctgtg ttttgattag ctataatttt
     4021 ttcatcatat tctatatttt tttctttatc atatttgtat ggtttaaatt gttgtagtat
     4081 tttcataaca tcaccccttg ttataaaata tgaactcata aacttgtctt atacttattt
     4141 aattgttaca attcttactt aaaatcactt gatattctat attttaaagc taaatttttt
     4201 acaactctac aatagaaatt tttcgaggtg atcatgtatg aacataagtc aagtgattaa
     4261 atatattagg gaaaatatga ataggtcaca aaaataattg tttgaaaaaa caaatataaa
     4321 tacaagtgtt atgaatataa tagagtctga tgaaagacca ataagagatg atgagttaat
     4381 aatatttgct aaaatttttg gtgtctctat caattatatt cttggtttat aagatacaga
     4441 aaaactaaat attgagcata atgacaatta aaactgaatt aattgtatta tatgtgtgag
     4501 gaatataaac tttacatacc acttagttaa tataaaacaa tatttatctt gtttcaatgc
     4561 ttctatacat atttccttta cataccactt agttaatata aaacaagata aaagtgaaat
     4621 atctgaatat atagaaaaac tctttacata ccacttagtt aatataaaac caccattaat
     4681 taaggggcaa tttagccttg caaatttctt tacataccac ttaattaata taaaactgtc
     4741 ccaaaatagc atcattactt taagttcagc attctttaca taccacttag ttaatataaa
     4801 accaatatcc atttctcctt ttccagcaat attaaatttc tttacatacc acttagttaa
     4861 tataaaacga gtgtatgctg aatatggttt aagaagtcct gacatacttt acataccact
     4921 tagttaatat aaaacgtctt ttcaatctgt tcaactacct taggttcatt cagctttaca
     4981 taccacttag ttaatataaa accttatact tccgttatta aacatcatat ctgctattat
     5041 ctttacatac cacttagtta atataaaacg gcaatccctt aaccatagcc aactatttaa
     5101 ttttgagctt tacataccac ttagttaata taaaacccca aaacaaattg agtatttcca
     5161 atacttacat ctatacaact ctcttaaatt tgcagtgaac catgagtagt gcaattgata
     5221 atacttatca cacatcttca atgcattata ttccaattgt taagccatat tttatcacaa
     5281 atttcgctca ctgcaaaact tctataattt tattatatta taaatatatt atttttgaat
     5341 atctgcacca atttgtggta taataaaagc aagaagaact acaatctatt tagcagtaga
     5401 gtggagttca tcataaacga tgttatttga atttatggaa ctttatttta aaatcaagtt
     5461 cccaaccact cttagtgcca gctttgagtg gttttttacg ttttctaaat aacttactta
     5521 ttaagtaaac tatatagcta gccagaatac tggctaatat actaagtaaa aaattatcca
     5581 tacataatca cctcccttct taaaatgggg aggataatcc tttgtttgta tgaactccac
     5641 tctatagatt gtagattaca tcttcttgct aaaattatta taacatatat tggtaaatta
     5701 tataattaaa aacactattt aaatgttatt ccaaacatcc ttataagcat atccactata
     5761 aacatctata aaataccatc cataggtttg atttggatgt ggttttgaat ctattcttac
     5821 agcatatact tttatatcat caaaatcatc aatataagca atattaactt ttaataaatc
     5881 aacaccaact ttatgttttc tggcacactc ttctaatgcc aattgttttg ctttttcttc
     5941 tgatatcatt tccttctttt tataatcatc tgggttacat acttgcataa ctccactaac
     6001 attatatgta taaactttgc tcatatcatt tttctctaca cagtatctaa catccccttc
     6061 agaattttct gtaacataac caaatgcata gaattgtttt cctgaaaatt caaaattctc
     6121 atctagtaag aatattacat cttcatcttc tggataattt tcattataat aaaatttctt
     6181 cacacctagc agttttttta ctgcattagc agcatcttct actgtatatt gtcttccagt
     6241 attagaatct cctatatttt ttgttacatt taaacactgt tgtaatatac tatcactcaa
     6301 atttcctata gcagttaatt tagttgtgct ttttaataca cttttattac tatcattatt
     6361 aactaacaca atagcattat ttttagcaac agtagaacca acaagagcat acactaaatc
     6421 agtaccactt gctatataaa attcttttac tccaccataa aatttattta ttatcttttt
     6481 gttagtatca tatctatctg tgccacctaa tctagttgaa ttagtatcat taactagttt
     6541 gtcattcatt gatgaagtac cacctattac ataactttct acaccagttg tattaaatgg
     6601 aacacttttt ccatcagtta atactatagc tgctttctct ctaactgcta caggtgcagc
     6661 actcattgca tcaggctctc ctttaaaagc attagtcaat atcactttat ttactttgtt
     6721 aattgaattt atttcttttg caacattata gcttgtctta attctatcac taccttgaag
     6781 tcttttaatc tctattcctt taccttttag aacagtttca gtatatttat caatagagtt
     6841 ttctcctcca attatatata ctttttttgc cttttctaat cgtttaagtg tagcatttgg
     6901 tatgttattt ttcttagtca aaagaattgg agcgtttgta gctcctgcaa gtccacttgc
     6961 acttaatcca tcagccattg tactatctgc attaattaat atagctgtag tatagttttg
     7021 tttatctgca attataccag tagtttcgta tttatctgca cctttaattg attcaatttt
     7081 atcaagagca tttgcagata tatggcaact agcaataaac atagataaag ataaccctat
     7141 tgttaataat tttttaattt tcataatatc ttccccttct aaatattttt aaatacaaat
     7201 tatctattca gttatactat aattatttgc agaataatga ggtgtccact ctattttttc
     7261 aaaatcctta tctgtactaa actcaagtct aactttttga cctttcttcc aagttccatt
     7321 tactgatgtt gatgtttgtg tctcgattat tacaccatct ttgtctatta gatttatatc
     7381 taagtaaaaa tcttgaagat ctaaatcact tgtattttct acagtagctt gataagtctt
     7441 ccaatcatat tcatctttta ctttttcaaa ttttatacct tttgccatag cattaacttt
     7501 ttcttttaaa tcattttctt catttacgat ttgtgcattg tcttttaata ctttaaactc
     7561 tttttcatct actttcaaac caaacttatc aactaaagtc aacaatatcg tgcttctctc
     7621 attataaggc ttttcccaaa gttctgaaaa ttttaattgg tcgtttgaat aatattttat
     7681 agcttccttt gaatcttcaa gagcttttat ataatctttt gccaacttgc caagttctgg
     7741 gctatcaaac tcagcatctt tatattttga tacagcatcc atttctactt ctacaaattt
     7801 ttcataagct tcattctgct ctaagtttag tgttccagct tcttctttag atgccaaatc
     7861 ccatcttttt tttaagcctt ttcctaaatc ctcaataaat tgcttgtcct tacctttgct
     7921 ttcatctttt gaatctcccc cactagaaca tgctaccata gaaactgata acataattat
     7981 cataaacaag cctagtattt ttttaaaatt acacatataa ttacccccta aaaattattc
     8041 tcaattgcat tatactatat tttctcattg aatgctgtcg attcatgcta aattttcata
     8101 ctatttttac aattatatca agttttaatt agcataaatt atcccattat atccttaata
     8161 taatatataa tagtacctaa ggagtgtgat tacttatatg agtgttgcca tatacttgag
     8221 aaaatcccgt gctgatgaag aagcagaaaa acaaggcgaa tttgaaactt taagtaggca
     8281 taaatcaacc ctactaaaac tagcaaaaga acaaagctta gatgtaattg agataaaaga
     8341 agaattagtt tctggtgaaa gtataataca tagacccaaa atgcttgaac ttctaaaaga
     8401 agtcgaagaa aataaatacg acgcagtctt agtaatggac ttagacagac ttggtcgtgg
     8461 agacatgaaa gaccaaggta tcatactaga aacatttaaa gaaagtaaga ccaaaatcat
     8521 cacacctaga aaaacatacg acctaacaga tgaatttgat gaagagtatt cagaatttga
     8581 agcatttatg gcaagaaaag aactaaaact tatatcaaga aggatgcaaa gaggtagaat
     8641 aaaatcagtt gaagaaggga atttcatagg aacttctgct ccatttggat atgatgctgt
     8701 aacaactggt agaaaagaaa gaatacttgt accaaataaa gatgctgata tagttaggac
     8761 tatctttgac ttatatatca atgaagatat gggttgtagt aaaatatcaa aatacctaaa
     8821 taacctaggc attaaaactg ctacaggtgc taattggtac aattctgcaa ttacaaatat
     8881 aataaaaaat aaagtctact gtggctacat tcagtggcaa aaaaaggact ataaaaaatc
     8941 taaaaatcca aataaaataa aaacagttaa attaagacct aaagatgaat ggattgaagc
     9001 aaaaggaaag catgaacctt taataagtga aataacatgg aaaaaggctc aaaatatact
     9061 caaaaaaaat ggtcatgtca gctatggtaa tcaaataaaa aatccacttg caggaatagt
     9121 gatttgtaaa aattgtggta ggccattagt atatagacca tatgcagacc acaactatat
     9181 aatttgttac catcctggtt gcaataaaag ttctcgattt gaatttattg aagctgctat
     9241 tttaaaatct ttggaagaca ctgtaaaaaa atatcaatta aaaacatctg acatagactt
     9301 agataaaaat aataaagaca gtaatataga atttcaaaag cgagtcttaa agggtttaga
     9361 aactgaatta aaagaattga gtaagcaaaa aaataagtta tatgatttat tagaacgtgg
     9421 aatatatgat gaagatacat ttattgaaag gtccaacaat ataagttcaa gaactgaaga
     9481 aattaaagat tcaattaaga cagtaaaaaa tagattgagt actgttaaaa aagacaatgc
     9541 taaaataata gaagatatta aaactgtgtt atcactttac catgattcag attcccttgg
     9601 aaaaaataaa ctcctaaaat ctgtaattga taaagcagtt tattataagt ctaaagaaca
     9661 aaaattggat tcttttgaat taatggttca tttgaaatta catgaagact agtaatacca
     9721 ataacatata aagagtagtg
//
LOCUS       DAFNNB010000144         9711 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0148, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000144 DAFNNB010000000
VERSION     DAFNNB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9711
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0148"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            90..422
                     /locus_tag="L3M60_002935"
     CDS             90..422
                     /locus_tag="L3M60_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HBN6227574.1"
                     /translation="MKVAYIFSSTNTHKILDKMIIPQLEQGTHGVDVLGMFFFMDNTL
                     FLSKGNPIGERLSKLHEKTDMIIMACDQCAIERGIENNLVDGATIGCFPNLYACLGSA
                     GVDQVITL"
     gene            584..769
                     /locus_tag="L3M60_002936"
     CDS             584..769
                     /locus_tag="L3M60_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227575.1"
                     /translation="MSEKYADKLEIKLYQAGKDFSYIKKYGIVTKGTLIINQKKKYDR
                     LNKDTIERAIVEAINNN"
     gene            797..1882
                     /locus_tag="L3M60_002937"
     CDS             797..1882
                     /locus_tag="L3M60_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBN6227576.1"
                     /translation="MNILEIVLNGVKDTIVASIVTLNGASGWLMISFILAGVLHNVLT
                     PRRFQKQLGNKKFSSLIKSTISGMFLPICSCGVIPLGISMYYSGAYLGPVLAFMTSTP
                     IINPIAVVLCLGLLGSKVTIIYLISGFLVPFLVGFLGNCFGKEELSLHNDLEEEVIEL
                     EEEKRSFMENMIDGLKWSFGELALTISKYVVMGMVFAGFITTIFPNSIIQKYLGNPGL
                     LSLGSIAILACVMYVCAVGHIPFIAAIVASGASPGIAITFLIAGAATNLPELISMFKL
                     IGKRTVIIYSVTLTLSSIIIGYITNLLLVNNNSVNLDATNSSIQLANKLMLNIPDSFE
                     YVCTILIILFAFKSIIPKMKEVIGNHA"
     gene            1875..2333
                     /locus_tag="L3M60_002938"
     CDS             1875..2333
                     /locus_tag="L3M60_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227577.1"
                     /translation="MLKKIVIISCIVVVLIILSKIVDDNIKEDASIPNVNKETLEYFR
                     KNYKEDIITCAEEDLNNDGKKDLVVIYKKSNNSNEMVVVVSDKNSHYITKPIPAPIEN
                     QTITFKNIDDKAPIEVIVSGSKNGNVGYAIYRVEGKKFVDLFGEDMDKCC"
     gene            2362..3447
                     /locus_tag="L3M60_002939"
     CDS             2362..3447
                     /locus_tag="L3M60_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6227578.1"
                     /translation="MKLSKKIQALILFGVMGTSILTGCSSKQKEEAKAGKDDYTITVG
                     YYNCDHMTAGPVAESAGIYKDLGLNVKTVGNGKVPEAMAAGKMDAGYIGTKGLVGAIP
                     KGSPITIAANNHTGGSEYLIVSKDIKEPKDLIGKKIATDMSDFLWTSDYGPETGLPTD
                     PSKYEVVNMDSDKDKYLALKTGKIQAFTSCDPWGSVAENDGAGKIIASTQYKEKANGK
                     EYNCCSFSLNKNFIKEHPDLAKKLVLAHTKAIEYIYTNPAEAAKIFAKYYNVEEEVAL
                     RTIYKKTVGEGRTLTWKVTGEEYKNNLQMYKDLKALDDIPKYEDTIDTSLLDSCGADD
                     FDKFIKEKVNPEFPEGMSYEDWKAKVL"
     gene            3452..3676
                     /locus_tag="L3M60_002940"
     CDS             3452..3676
                     /locus_tag="L3M60_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227579.1"
                     /translation="MENIKNIIILFIIILGSIFIIFFNFEDKEEIKGKLRIGVSDDTS
                     GFVINYMVNRDYFENIKVSDIMDIFTINDC"
     gene            3686..4249
                     /locus_tag="L3M60_002941"
     CDS             3686..4249
                     /locus_tag="L3M60_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227580.1"
                     /translation="MQWALSSEEIDIAIICKDAAKQYVNVNSGFEIVGTVVQNSDIFL
                     LSDNNPKKMGVIQNRDYQSELVKKYYKNVEVAPLLGTALAYGLESNLVDGVVIDCIKS
                     IGLEGKRKSTTKLGDYDTYVLVVSKEFKKSKAYSDFITLYNKSVDALKNKATFKKALK
                     EYRNIRNIDMEEISNWKLKFLKIKAID"
     gene            4210..4839
                     /locus_tag="L3M60_002942"
     CDS             4210..4839
                     /locus_tag="L3M60_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227581.1"
                     /translation="MEIKISKDKSDRLTKKKTDGSMDILYKIILLVIIFLVWHFAAKD
                     IGSSLLLPMPVDVIKGFFFCVTDAETVTNLFITLQRVLKGFIYALLFGLPIGFIMGFS
                     KTFERVLSPVVDSVRQVPIMAWVPLTIVWFGIGDGPTIFLIAFSGVFPIILNTIQGVR
                     AISKDYYNAARSMGASPIVIFTNVIIPASLPDILTGSRIAISTGWMSVI"
     gene            4879..5016
                     /locus_tag="L3M60_002943"
     CDS             4879..5016
                     /locus_tag="L3M60_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6227582.1"
                     /translation="MVQAQTLMHTDVLIGLMIFAALIGFIIDRVLKFINKILCRWRFA
                     D"
     gene            5016..5768
                     /locus_tag="L3M60_002944"
     CDS             5016..5768
                     /locus_tag="L3M60_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227583.1"
                     /translation="MKLSVRDINKTFVNNRVNTKVLEDISMDIDDGQFVCLLGPSGCG
                     KTTLLTIIGGFQKSESGDVFINDKKVKKPGIDRAFIFQNYALFPWKTIRGNVLFPMKQ
                     QKIPKEKREEMLEELLVMSDLKGKEKLFPHQLSGGMKQRVAMIRALACNPEILLMDEP
                     LGAVDFQMRQNLQEELERIWIKKKITALMVTHDVDEAVYMSDRVIVMSRDKGRIIDDI
                     NIDIPRPRIRGSQKYEEYKNKLTDTLSKCYEV"
     gene            5781..6266
                     /locus_tag="L3M60_002945"
     CDS             5781..6266
                     /locus_tag="L3M60_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227584.1"
                     /translation="MSQIEKYNSLDNTQQKIRISIMDMIIDKGSSVTLQEVTEYISKK
                     LNMEKEYIESTLQYFIDKNIMVVDGDSINFIYPVSALPTNHKVTLRDNRSFSAMCAID
                     AIGTSCTFNQDVRINSICSVTGREIEIVIKNEKIEYVNNPSLRVLHINLDKYSNWAAN
                     C"
     gene            6273..6422
                     /locus_tag="L3M60_002946"
     CDS             6273..6422
                     /locus_tag="L3M60_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227585.1"
                     /translation="MNFFWTKSDFDAWTIEAGLSDDEDIYCLDINEAIVESYKIFKLK
                     QKIIL"
     gene            complement(6554..7375)
                     /gene="yidA"
                     /locus_tag="L3M60_002947"
     CDS             complement(6554..7375)
                     /gene="yidA"
                     /locus_tag="L3M60_002947"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBN6227586.1"
                     /translation="MYKLIALDIDGTILNTQKRITPEVFESIQEAKRAGAKVVITTGR
                     PLPGVKELLNQLNLTDEGDYVICFNGAIIQEVKSEKIIHDVEMTLDDFDFIYNNVCKK
                     YKTKIHINTMTNLITPNETPGKYTLHEAKLNDIEVKYIQKDKIDESIKICKIMIVDEP
                     EKLEEIIQQLPKNLFNKYTIVRSAPFYLEFLGKTTNKGTALKTLCANLNIPIENAIAV
                     GDEENDQHMIKYAGLGVAMGNARNSIKEIADYVTDTNNENGVAKVINKYILNKAI"
     gene            7869..9563
                     /locus_tag="L3M60_002948"
     CDS             7869..9563
                     /locus_tag="L3M60_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HBN6227587.1"
                     /translation="MKFSEFKYERPNYNSMKKEFLGCVEDINNSHNYKEQQKNIHKLN
                     LLRNKIETLSNIALIRYSTDTLNKFYQEEKDYWDEYMPLYEELNSHFYNAIVNSKFKC
                     DLIKEFGEQFFTIVEYSLKSFSKEIISELQEENKLCSEYTRLLASAEIMFDGKIRNLS
                     GMGKFMYSKNRKIRELANKAYYNFFEKNETKFDDIFDKLVKLRDKISKKLGFEDFVEL
                     GYIRMMRSDYREDMIKSVRKQVLKYVVPMANELYEKQAKRIGLEYLSYIDEGVEFLTG
                     NASLKGDSRYIIQNGKRMYSELSKETNEFFNFMLENELMDLETKKGKGAGGYCTYIPD
                     YKSPFIFSNFNQTADDIDVLTHEAGHAFQLYMSRWIDMPEINFPTLDSCEIHSMSMEF
                     ITWPWMDLFFKEDTDKYKFTHLSSAIKFIPYGVIVDEFQHYIYKNPNIDKSKRKEIWR
                     FLEKKYLPHRKYGDNSFLDRGCWWFKQGHIFKNPFYYIDYVLAQICALQFWKKMIQDR
                     DTGWKDYINICKVGGTKSFLDIVSMGNLYSPFDDGCIESIIGDVKSWFDEINDSKL"
BASE COUNT         3784 a         1015 c         1704 g         3208 t
ORIGIN      
        1 ctattttaca gataaataag aataaactta taatataatt gtatctgtaa aattttgtaa
       61 aaatataaaa agtatagggg ggaataaaaa tgaaagtagc ttatattttt agtagtacta
      121 atacacacaa aattctagac aaaatgataa taccacaact tgaacaagga actcatggag
      181 tagatgttct agggatgttt ttctttatgg ataataccct atttttatct aaaggtaatc
      241 ctatagggga gagattaagt aaattacatg aaaaaactga tatgataata atggcttgtg
      301 accaatgtgc tatagaaaga ggtatagaga ataatttggt tgatggagct acaattggat
      361 gtttcccaaa cctatatgct tgtttaggca gtgcaggtgt ggaccaagtt ataacgttat
      421 aaattagtgt aggatacaat ccatagaaaa atttattttc tgtggattat tttaagtaaa
      481 agataaattg tttaaagtag gggatgagca aatgtctaaa attttagttg aatttataaa
      541 cacttgacca tcttgtgatg agcatggagt gtttataaaa aaaatgtcag aaaagtatgc
      601 tgataaattg gaaataaaat tatatcaagc aggcaaagat ttttcatata taaaaaaata
      661 tgggattgta acaaaaggaa ctttaataat aaatcagaaa aagaaatatg atagattaaa
      721 taaagacaca attgaaaggg caatagtaga agctataaac aataattaaa agtttaaaaa
      781 ttaggagtat caaaaaatga atatattaga aattgtttta aatggagtta aagatactat
      841 agtagcttcc atagttacat taaatggagc atcaggatgg cttatgataa gttttatatt
      901 agcaggagtg cttcacaatg ttttaactcc tagaagattt caaaaacaac ttggaaataa
      961 gaaattttcc tctttgatta aaagtactat ctcaggaatg tttttaccaa tatgtagttg
     1021 tggggtaata ccacttggaa taagcatgta ttattcaggg gcatatttag gaccggtact
     1081 tgcttttatg acatcaactc caataataaa tccaatagct gtagttcttt gtctgggtct
     1141 tttagggtcc aaagttacaa ttatttatct aatatctggt tttttagtac catttttagt
     1201 aggattttta ggaaattgtt ttggaaaaga agaattatca cttcataatg atttagagga
     1261 agaagtaata gaattagaag aagaaaaaag aagttttatg gaaaatatga ttgatggtct
     1321 taaatggtct ttcggggagt tggctcttac tattagtaaa tatgtagtaa tgggtatggt
     1381 ttttgcagga tttataacaa ccatttttcc aaatagtata attcaaaaat atttaggaaa
     1441 tccaggtttg ttatctcttg gaagtatagc aatacttgca tgtgttatgt atgtatgtgc
     1501 tgtaggacat ataccgttta tagcagccat tgttgcaagt ggagcatctc ctgggatagc
     1561 tataacattt ctaatagctg gagcagctac aaatctacca gaacttataa gtatgtttaa
     1621 acttattgga aaaagaactg ttattatata ttctgtaact ttgacactgt cttcaataat
     1681 aattggatat ataacaaatt tacttttagt gaacaataac tcagtaaatt tagatgcaac
     1741 aaattcttca atacagttgg caaataaact aatgttaaat atacctgata gttttgagta
     1801 tgtatgtacg attttaatta ttttatttgc atttaaatca ataataccaa agatgaaaga
     1861 ggtcatagga aatcatgctt aaaaagatag ttataatatc ctgtatagta gttgtgttaa
     1921 tcattttatc aaaaatagtt gatgataaca tcaaagaaga tgcctctatt ccaaatgtga
     1981 ataaagaaac attagaatat tttagaaaaa attataaaga agacataata acttgtgctg
     2041 aagaagactt aaataatgat gggaaaaaag atttagtagt tatatataaa aaaagcaata
     2101 attcaaatga aatggtagtt gtggtttctg ataaaaatag ccattatata acaaagccaa
     2161 taccagcacc tatagagaat caaactataa cctttaaaaa tattgatgat aaagcaccaa
     2221 tagaagtaat agtatcaggg tctaaaaatg gtaatgtagg atatgctatt tacagagtag
     2281 agggtaagaa atttgttgat ttatttggcg aagatatgga caaatgttgc taaaaataat
     2341 acataaaggt gggaaattta tatgaaatta tctaagaaga ttcaagcatt aatcttattt
     2401 ggtgtaatgg gtacatctat attaacagga tgtagtagta aacaaaaaga agaagcaaaa
     2461 gctggaaagg atgattatac aataacagta ggttattata attgtgacca tatgactgcg
     2521 ggaccagtag cagaatcagc aggaatatat aaagatttag gtcttaatgt aaaaactgtt
     2581 ggaaatggta aagttcctga agctatggca gcaggaaaaa tggatgcagg ttacatagga
     2641 actaaaggtt tagttggagc aatacctaag ggttcaccaa taacaatagc agctaacaac
     2701 catactggtg gttctgaata tttaatagtt tcaaaagata taaaagaacc taaagattta
     2761 attggtaaaa aaattgcaac agatatgagt gatttcttat ggacaagtga ttatggtcca
     2821 gaaacaggtt taccaactga cccatcaaaa tatgaagttg taaatatgga ttcagacaaa
     2881 gataaatatt tagctcttaa aactgggaaa attcaagctt ttacatcttg tgacccttgg
     2941 ggttctgtag ctgaaaatga cggtgcagga aaaataatag catctactca atataaagaa
     3001 aaagctaatg gcaaagaata taactgttgt tcattctcat tgaacaaaaa ctttataaaa
     3061 gaacatccag atttagcaaa aaaattggtt ctagcacata caaaagcaat agaatatata
     3121 tatacaaatc cagctgaagc agctaaaata tttgcaaaat attataatgt agaagaagaa
     3181 gttgcactta gaactatcta taagaagact gttggagaag gtagaactct tacttggaaa
     3241 gttacaggtg aagaatataa aaacaattta caaatgtata aagatttaaa ggctttagat
     3301 gatataccta aatatgaaga tacgattgat acaagcttat tagactcttg tggagctgat
     3361 gattttgaca aatttataaa agaaaaagtt aacccagagt ttcctgaagg tatgagttat
     3421 gaagattgga aagcaaaggt attataaata aatggagaat ataaaaaata taataatact
     3481 ttttatcatt attttaggaa gtatatttat aatatttttt aattttgaag ataaagaaga
     3541 aataaaagga aaactaagaa taggggtatc tgatgatacc tctggttttg taataaatta
     3601 tatggtaaat agagactact ttgaaaatat aaaagtaagt gatataatgg atatttttac
     3661 aattaatgat tgctgagcaa gtacaatgca gtgggctctg agttcagagg aaatagatat
     3721 agcaatcata tgtaaagatg ctgcaaaaca atatgtaaat gtaaatagtg gatttgaaat
     3781 tgttggtact gtagttcaaa attctgatat atttctatta agtgataata atcctaaaaa
     3841 gatgggagtc attcaaaata gagattacca gtcagaattg gttaaaaaat actacaagaa
     3901 tgtggaagta gctcccttat tagggactgc acttgcatat ggattagaaa gtaatttagt
     3961 agacggtgtt gtaatagatt gcataaagtc aattgggtta gagggtaaaa gaaaatctac
     4021 aactaaatta ggagattatg atacctatgt attagttgta agtaaagagt ttaaaaaaag
     4081 taaagcatat agtgatttta taacacttta caataaaagt gttgatgctt taaagaataa
     4141 agctacattt aagaaagcat taaaagagta caggaatata agaaatatag atatggagga
     4201 aataagcaat tggaaattaa aatttctaaa gataaaagcg atagattaac taagaaaaaa
     4261 actgatggta gtatggatat tttatataaa ataatactat tagttattat atttttagtc
     4321 tggcattttg cagcaaaaga tatagggagt tctttattac ttccaatgcc agtagatgta
     4381 ataaaaggtt tctttttctg tgtgacagat gcagagacag ttactaatct ttttataaca
     4441 ttgcagagag tacttaaagg ttttatctat gcattattgt ttgggcttcc aataggattt
     4501 attatgggat tttctaaaac ctttgaaagg gttttatctc cagtagtaga ttctgtaaga
     4561 caagttccaa taatggcatg ggttcctctt actatagtgt ggtttggtat aggagatggt
     4621 ccaacaatat ttttaatagc tttttcaggg gtcttcccta taattttaaa tactattcaa
     4681 ggagtaagag caatatcaaa ggattattat aatgcagcta gaagtatggg agcatcacca
     4741 attgtgatat ttacaaatgt aattattcca gcatctcttc cagatatact aactggttca
     4801 agaattgcta taagtacagg atggatgtct gttatctgag ccgagtttat agctacgagt
     4861 gccggtctcg gttactcaat ggtacaagct caaacattaa tgcatacaga tgtattaatt
     4921 gggcttatga tatttgcagc tttaataggg tttataatag atagggtatt aaagtttata
     4981 aataaaatcc tttgtagatg gaggtttgca gattaatgaa gttaagtgta agagatataa
     5041 ataagacatt tgtaaataat agagtaaata caaaagtttt agaagatata agtatggata
     5101 tagatgatgg acaatttgtc tgtcttttag gaccatcagg ttgtggaaaa acaactttac
     5161 ttactataat aggtggattt caaaaatcag aaagtggaga tgtttttata aatgataaaa
     5221 aagttaaaaa accaggaata gatagagctt ttatatttca aaattatgct ttatttcctt
     5281 ggaaaactat aagaggcaat gttctatttc ctatgaaaca gcagaaaata ccaaaagaaa
     5341 aaagagaaga aatgctagaa gaacttttgg ttatgtcaga cttgaaagga aaggaaaaac
     5401 tgtttccaca tcaattatct ggaggaatga aacaaagagt ggccatgata agagctttag
     5461 catgtaatcc agagatatta ctaatggatg aaccattagg ggctgtagat tttcaaatga
     5521 gacaaaattt acaggaagag ctagaacgaa tatggataaa gaagaaaata actgcattaa
     5581 tggtaactca tgatgttgat gaagctgtat atatgagtga tagagttata gtaatgtcga
     5641 gagataaagg aagaattatt gatgatataa atatagatat tccaagacct agaattagag
     5701 gaagccaaaa atatgaagag tacaaaaaca aattaactga tactttatca aagtgttatg
     5761 aagtttaaag aggagtagac atgagtcaaa tagaaaaata caatagtttg gataatacac
     5821 aacagaaaat aagaatctct attatggata tgataataga taaaggttct agtgttacac
     5881 ttcaagaagt gactgaatat ataagtaaaa agttgaatat ggaaaaagaa tatatagaaa
     5941 gtacactaca atattttatt gataaaaata taatggttgt tgatggagat agtataaatt
     6001 ttatatatcc tgtatcagca ttgccaacaa atcataaagt aactttgaga gataatagaa
     6061 gttttagtgc aatgtgtgct atagatgcta tagggacatc ttgtaccttt aaccaagatg
     6121 taagaataaa ctctatttgt agtgttacag gtagagagat agaaatagtt ataaaaaatg
     6181 aaaagattga gtatgtgaat aatccgagct taagggtgct tcatataaat cttgataagt
     6241 attcaaattg ggctgctaat tgttgaaata taatgaattt cttctggacg aagagtgatt
     6301 ttgatgcttg gacaatcgaa gctgggttat ctgatgacga agatatatat tgtttagata
     6361 ttaatgaggc aatagtagaa tcgtataaaa tatttaaact caaacaaaag ataatattgt
     6421 agatatacat aaaaaactaa aaattacctt taggtaaaaa tgatagataa tatccatact
     6481 gtataaatta gtatagagta tggatatgaa aagtatttaa atctaaaggt atatttttag
     6541 tttttttata tgattagata gccttattaa gtatatattt atttataacc tttgcaactc
     6601 cattttcatt attagtatca gtaacataat ctgcaatttc ctttatacta ttacgtgcat
     6661 ttcccatagc cacaccaaga cctgcatact ttatcatatg ttggtcattt tcttcatcgc
     6721 caacagcaat agcattttct ataggtatgt ttagattagc acataatgtt ttaagtgcag
     6781 ttcctttatt agtagtttta cctaaaaatt ctagataaaa aggagcagac cttactatag
     6841 tatatttatt gaataaattt ttaggtagct gttgtatgat ttcttccaat ttttctggtt
     6901 catcaactat cataatttta cagattttaa tagattcatc aattttatct ttttgtatat
     6961 atttgacttc tatatcattt aatttcgctt catgaagtgt atattttcca ggagtttcat
     7021 ttggtgtaat taaatttgtc attgtattta tgtgtatttt agtcttatat tttttacaaa
     7081 cgttattata tataaaatca aaatcatcta aagtcatttc tacatcatgt ataattttct
     7141 cacttttaac ctcctgaata attgctccat taaagcatat aacataatcc ccttcatctg
     7201 ttaaatttag ttgatttaga agctctttta caccaggaag aggtctgcct gttgttataa
     7261 ctacttttgc cccagccctt tttgcctctt gaatggactc aaagacttca ggagtaatcc
     7321 ttttttgtgt atttaatatt gttccatcaa tgtctagtgc gattaatttg tacatatcaa
     7381 cctccaataa attatatact caaatttcct gtgaaaacgt tggcacgact agttttgaac
     7441 aaagtttcag tgggaaatat ggtttgtttt gtatactatt atatcaagag ataagtaatt
     7501 tagaaagttt aataatatta attttttgat aaaaatatta cctatgtgag atgagagtgt
     7561 aaaaactttg taatcaataa ttccaaaatt tttaattaca aataatggta tcgtttgcat
     7621 tgttaatata aatataatgt atattatata gttagaagtt tgaataaata gactgatggt
     7681 aatttgttat attagttagt tttgtgttat aaaatattgt aattccatta atcatatgtt
     7741 attattcaac atgattgcaa tataaagaat atttaatata tataataatt tataaaagag
     7801 tattacatag tacatgtaaa gttatattta taaatacaat aaaataaagt tgtaaaggag
     7861 tgttttaaat gaagttttca gagtttaagt atgaaagacc caattataat agtatgaaaa
     7921 aagagttttt gggttgtgta gaagatataa ataattctca taattataaa gagcaacaaa
     7981 aaaatataca taagcttaat ttattgagaa ataaaataga aacgttgtca aatatagcat
     8041 tgataagata tagcacagac acacttaata aattctacca agaagaaaaa gattattggg
     8101 atgaatatat gcctttatat gaagaattaa attcacattt ttataatgct attgtaaatt
     8161 caaagtttaa atgtgatttg ataaaagagt ttggagaaca atttttcaca atagtagagt
     8221 attctttaaa aagtttttct aaagaaataa taagtgaatt acaggaagaa aataaattgt
     8281 gttcagagta tactagactt ttagcctcag ctgagataat gtttgatggg aaaataagaa
     8341 atttatctgg tatggggaaa tttatgtatt ctaagaatag aaaaataaga gaactagcaa
     8401 acaaagcata ttataatttc tttgaaaaaa atgaaactaa atttgatgac atatttgata
     8461 aactggttaa acttagagat aagatatcta aaaaacttgg ttttgaagat tttgtagagc
     8521 ttggatacat tagaatgatg agaagtgatt acagagaaga tatgataaaa agtgttagaa
     8581 aacaagtatt aaaatatgtg gttccaatgg ctaatgagct ttatgaaaaa caagctaaaa
     8641 gaattggtct agaatattta agttatattg atgaaggtgt ggaattttta acaggaaatg
     8701 caagcttaaa aggagattca agatacataa ttcaaaatgg aaaaaggatg tattctgaat
     8761 tgtcaaaaga gacgaatgaa ttttttaatt ttatgttaga aaatgagctt atggatttag
     8821 aaacgaagaa aggaaaagga gccggagggt attgtactta tataccagat tacaagtcac
     8881 cattcatttt ttctaatttt aatcaaactg ctgatgatat agatgtttta acacatgagg
     8941 caggacatgc atttcagttg tatatgtcta gatggattga tatgccagaa attaattttc
     9001 caacgcttga cagttgtgaa atccattcaa tgagtatgga atttataaca tggccatgga
     9061 tggacttatt ttttaaagaa gatacagata agtacaaatt tactcactta tcatctgcaa
     9121 ttaaatttat accttatgga gtaattgttg atgaatttca acactatatt tacaaaaatc
     9181 ctaatataga taagtcaaag agaaaagaaa tctggaggtt tttagagaaa aaatacttac
     9241 ctcatagaaa atatggtgat aattcttttt tagatagggg ctgttggtgg tttaaacaag
     9301 gacatatatt taaaaatccg ttttattata tagattatgt attagctcaa atttgtgcgt
     9361 tacaattttg gaaaaaaatg attcaagata gagatacagg atggaaggat tatataaata
     9421 tctgtaaagt tggaggaaca aaatcatttt tagatattgt gagtatggga aatttgtatt
     9481 ctccatttga tgatggatgt attgagtcta ttataggtga tgttaaatct tggtttgatg
     9541 aaataaatga cagcaaatta taaaaaatta tgagaatttt attgtataaa attaaaaaat
     9601 taaaaaatat ataaaaaacg attatttgtt gtgaaaatag tcttgttttt aactaaattg
     9661 ataaaataaa ctaaaaaaga tttgcactta aaaaaaaaag aaaatacaag a
//
LOCUS       DAFNNB010000145         9600 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0149, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000145 DAFNNB010000000
VERSION     DAFNNB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9600
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0149"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            263..763
                     /locus_tag="L3M60_002949"
     CDS             263..763
                     /locus_tag="L3M60_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227588.1"
                     /translation="MIEGIKFIKAEKKYIYSYWQTFDKIAKERKYLAMDEAFPFEETV
                     EFIKNTITKNLPQLFVIDLESDNCIGWCDVLPKTEKVGYLGMGILKEYREKGIGSSLL
                     KQIINLSKEYGYEKIELDVLKSNSRAIHVYKSLGFVEVNTISSGFTWNDRPVKEEVIQ
                     MELTLI"
     gene            complement(1004..1783)
                     /locus_tag="L3M60_002950"
     CDS             complement(1004..1783)
                     /locus_tag="L3M60_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227589.1"
                     /translation="MNKNILNNSFPEVPESFHNSLNKTLNSLPEREENYKMINNKTHK
                     LPFGKGLVATLAITLILGTTALAVGQISSLVAKSSTKATYTSIPTTEQVKKDFNFTPN
                     IVSEFSNGYKFKGAYTVNEEALDKEDNVLGELKALSFEYKNGNDEISLKTANNVLGNS
                     SDNLKVVDTYKDINISYKAFAQKFVPEGYKMTEQDKVDEKSGKYTFSVGPNNESGKVE
                     VNEFKFLTWKQDGVYYSFTVQDSNLSMNELVKMVHEVIDAK"
     gene            complement(1780..2259)
                     /locus_tag="L3M60_002951"
     CDS             complement(1780..2259)
                     /locus_tag="L3M60_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HBN6227590.1"
                     /translation="MDKTTFTNNILESEQTLYRVSKSILGNDQDCEDAVNNAILKAYE
                     KLDSLKEEQYFKTWLIRIVINECNSLRRKRLKFLSFEDVFKNKKIDEKDDYSDLYTAI
                     QSLPKKIKIPIVLYYIEGYSVDEVKEILDIPQGTVKSRLSRGRRLLKTKLENTEVII"
     gene            complement(2788..4191)
                     /locus_tag="L3M60_002952"
     CDS             complement(2788..4191)
                     /locus_tag="L3M60_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6227591.1"
                     /translation="MKMLNNFFKKINNPAPVFAQTKMERDNQTDDSLSTKPKTTFRDV
                     AGLDEVKEELFEIVDFMKSPQKYQKMGAKIPKGVLFYGPPGTGKTLLASAVAGETNSS
                     FFNVTGSEFVEKYVGVGAKRVRTLFEKARKEAPSIIFIDEIDAVGAKRHLESNNEKDQ
                     TLNQLLVEMDGFNKDSNVLIIGATNRLDLLDEALLRPGRFDRHIHIGAPNYHTRFEIL
                     KVHTDDKPIDKSVNLELLAKKTHGFNGAHLSNIANEAAIFAVRDDSECITSEHFDKAL
                     ERVIAGLESKNSALVEKEKKIVAYHEAGHALVSDIVGICPIQKISIVPRGQALGYVLQ
                     LPDEDRYIYTKDELIGKIKILLAGKASEELIFNHKSTGAKDDLKKVTEIANQMVCEYG
                     MSNLGFMTIDGNDKTFLCDKVQKEANRIVEVCYKETLEMLKDNLEDLHSVSKFLFEKE
                     TMTHEELKDLIGKEAVN"
     misc_feature    4896..5038
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            5060..5569
                     /locus_tag="L3M60_002953"
     CDS             5060..5569
                     /locus_tag="L3M60_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBN6227592.1"
                     /translation="MSKELAINEQIKDKEIRVLSQTGEQLGVMPTKEAQAMANSKNLD
                     LVQISPNANPPVCKIMDYGKFRYEQARKEKEAKKKQKTIVVKEVRLRPGIEQNDLNTK
                     ANNAIKFLKKGDKVKVELRFRGRELGHKDIGKEVMLKFLDIIKEFGEPTKAPAFEGNN
                     MVVIIDPKK"
     gene            5597..5791
                     /gene="rpmI"
                     /locus_tag="L3M60_002954"
     CDS             5597..5791
                     /gene="rpmI"
                     /locus_tag="L3M60_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBN6227593.1"
                     /translation="MPKMKTHRGAAKRLKKTGTGKLKRAKAYKKHILTKKSAKTKMNL
                     RKSTLVSDGDAKRIAQLLPY"
     gene            5847..6203
                     /gene="rplT"
                     /locus_tag="L3M60_002955"
     CDS             5847..6203
                     /gene="rplT"
                     /locus_tag="L3M60_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBN6227594.1"
                     /translation="MARVKKAMNARKKHKKILKLAKGFRGSRSKLYRPANTFVMKALK
                     NAYIGRKLKKRDFRKLWIQRINAAARMNGISYSRLMNGLKLSGIEVNRKMLSEMAIQD
                     PEGFAKLAEVAKAKLA"
     gene            6504..7049
                     /locus_tag="L3M60_002956"
     CDS             6504..7049
                     /locus_tag="L3M60_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227595.1"
                     /translation="MNDKLMDCFVNPIKCKLLLEIFSNNRTTAKQLGEKFQDISKATL
                     YRYLNKMLKDDIIKVVEENKIRGTVEKVYAMKLDIADDVNKIIDENSGEEYAQLFTQY
                     MMGFLREFYEYAERKEKDILKDGSVFSVCPVYASIDEIVEASKKIASILEPLKNNSHE
                     EGRRMHSIGVIITPPKEDEGK"
     gene            7234..8151
                     /locus_tag="L3M60_002957"
     CDS             7234..8151
                     /locus_tag="L3M60_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBN6227596.1"
                     /translation="MRLITRSDFDGLVCGALLKEAGIIDSWKFAHPKDLQDGLVEVTE
                     DDCLANVPFVEGCGLWFDHHSSEFERNKLEGRYKGESRIAPSCARIIYEYYGGNERFS
                     HFNEMLEAVDKVDSGNLTIEEVQNPSGWILIGYLMDPRTGLGRWRNFTISNYQLMEKL
                     IDACRTLSTKEILELPDVKERIEVYFEQTNKFKEMIAKYTRVEGDVIISDLRGVEPIY
                     TGNRFMIYSMYPEQNISAWIVNGKGGKGCSVAVGYSILNRTSSVDVGSLMLKYGGGGH
                     KAVGTCQFNDEQIDELLPKLLDELVNTKN"
     gene            8539..9567
                     /locus_tag="L3M60_002958"
                     /pseudo
     CDS             8539..9567
                     /locus_tag="L3M60_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
BASE COUNT         3623 a         1106 c         1551 g         3320 t
ORIGIN      
        1 tttttttata tattttatta tactttaaat taaatttcaa aatgattaag tatatatact
       61 caaaatatta aagaactata tttattatac tattaaaaat tattttaata gagatacaaa
      121 agatagatat attggtttta attttataga aatatacaaa gatattttaa gctaatatat
      181 agtaatttat atagcattat aaattagaat catataaaat aagttaacta caatcataaa
      241 ttaaaaaagg ggaaatgtta agttgataga aggcataaaa tttataaaag cagaaaaaaa
      301 gtatatttat tcgtattggc aaacttttga taaaatagca aaagagagga aatatctagc
      361 tatggatgag gcctttcctt ttgaagaaac agttgaattt ataaaaaata ctataactaa
      421 aaacttacca caactgtttg taattgattt agaatcagat aactgtattg gttggtgcga
      481 cgttttacct aaaactgaaa aagttggtta tttaggcatg ggtattctta aagagtatag
      541 ggaaaaaggc attggaagta gtcttttaaa acaaattatt aatttatcaa aagaatatgg
      601 atatgaaaaa attgagcttg atgtgttgaa aagtaatagt agagctattc atgtttataa
      661 atccttaggg tttgtagagg taaatacaat atctagtggt tttacatgga atgatagacc
      721 tgtaaaagaa gaggtaattc aaatggagct tacacttata taaaataaag ttaaacccct
      781 taaaattcca tatacactta tataaaataa aattaaagcc cttaaagttc catataaatt
      841 taagggcttt ttaatagtgc ttcttttgaa agtagttaaa tatctaaaat tttatatatt
      901 aaatttaaag aaaatataaa gatggaagat acaaccatgt ttgctaataa aaaaattaaa
      961 gtcgtaaata ataaactcat aaacaactta ttattactta tgtttatttt gcatctatta
     1021 cttcatgaac cattttaact aattcattca tagataaatt tgagtcttga actgtaaaag
     1081 aatagtaaac tccatcttgt ttccatgtca aaaacttaaa ttcattaact tcaactttac
     1141 ctgattcatt atttggacct acactaaatg tgtatttacc tgatttttca tcaactttat
     1201 cctgttctgt cattttataa ccttctggta caaacttttg tgcaaatgct ttataagaaa
     1261 tattaatatc tttgtaagta tctacaactt ttagattgtc agaactattt ccaagtacat
     1321 tatttgctgt tttaagtgat atttcatcat taccattttt atactcaaaa ctaagtgctt
     1381 ttaattctcc taaaacatta tcttctttgt caagtgcttc ttcatttaca gtatatgcac
     1441 ctttaaattt atatccatta ctaaattcag aaactatatt aggggtaaag ttaaagtctt
     1501 tttttacttg ttctgtagtt gggatagatg tataggtagc tttagtactt gattttgcaa
     1561 ctagactaga tatctgccct actgcaagag ctgttgtgcc caatattaaa gtaatggcca
     1621 aagttgcaac taagcctttt ccaaatggta atttatgtgt tttattatta atcattttat
     1681 aattttcctc cctttctggt aaactattta gtgttttatt taaactatta tgaaaacttt
     1741 ctggaacttc tggaaacgaa ttatttaata tatttttatt catatgatta cctccgtgtt
     1801 ttctaattta gtttttaata atcttcttcc tcttgataat ctacttttta cagttccttg
     1861 aggtatatct aatatttcct taacttcatc tactgaataa ccttcaatat agtaaaggac
     1921 tattggaatt ttaatttttt taggtagact ttgaatagca gtatacaaat cactataatc
     1981 atctttttca tcaatttttt tgtttttgaa gacgtcttca aatgatagaa attttaatcg
     2041 tttacgtctt aatgaattac attcatttat gactattcta atcaaccagg ttttaaagta
     2101 ttgttcttct ttaagagaat ccaacttttc atatgctttt aaaatagcat tgttgacggc
     2161 atcttcacag tcttggtcgt ttcctagaat agacttagat actcgataca aagtctgttc
     2221 tgattcaagt atattgtttg taaatgtagt tttatccaat tgagttccta cctttctatg
     2281 caaatcgtag cgctactttt ttgcctttca cttattagat aaaattttac tatagatggt
     2341 tcactttctt tataattatt tgataaattt aagtttaaaa gttttgtcta ttctatacaa
     2401 atattctata atatattttt ttacagtcat atataaagcc atgtatatat gaaactttag
     2461 aagctattta tatatttagt tttatagtaa gtgtttatat tgagtaatta gaaaataata
     2521 agtatttatg ttgagtagtt ggaaaataaa taatattttt gaattattta attcatgtat
     2581 agatttaaaa taagttcttt aaatagcgac tttaaataga tataagtcat aaattaaata
     2641 gacaaaatta aaagatggaa gtatatcaaa atgaattttt tagttcataa ggcgtttttt
     2701 gtatgaaatc aaataagttt ttataatttt aacaataaaa agaggcttat ctctattttg
     2761 agatagcctc atatttttaa aactatacta gtttacagct tcctttccta tcaaatcttt
     2821 taactcttca tgagtcatag tttctttttc aaataagaat ttagaaacag aatgtaaatc
     2881 ttcaagatta tcctttaaca tctctaaagt ctctttataa caaacttcaa ctatacgatt
     2941 tgcttccttt tgaactttat cacataagaa agtcttatca tttccatcaa tagtcataaa
     3001 ccctaaatta ctcataccgt attcacaaac catttgatta gctatttcag tgactttctt
     3061 taagtcgtcc tttgcaccag tagatttatg attaaatatt aattcttctg aagcctttcc
     3121 agcaagtaat atttttattt ttccaatcaa ttcatcctta gtataaatat atctatcttc
     3181 atcaggtaat tgaagaacat aacctaatgc ttgtcctcta ggaactatag atattttttg
     3241 tataggacaa attcctacta tatcacttac aagagcatgt ccagcttcat gatacgctac
     3301 tatttttttc tctttttcaa ccaatgcaga gtttttagat tctagccctg caataactct
     3361 ttcaagtgcc ttgtcaaaat gttctgatgt aatacactca ctgtcatctc taactgcaaa
     3421 tatagctgct tcatttgcta tatttgaaag atgagcccca ttaaaaccat gagttttttt
     3481 agctaaaagt tctagattta cagatttgtc tataggtttg tcatctgtat gtacctttag
     3541 aatttcaaat ctagtgtgat aatttggtgc acctatatga atgtgtctgt caaatcttcc
     3601 tggtctaagt aaagcttcat ctaataagtc aagtctattg gtagcaccta ttattaatac
     3661 attggaatct ttattaaatc catccatttc aactaaaagt tggtttagtg tttggtcttt
     3721 ttcgttgtta ctttcaagat gtctttttgc tcccacagca tctatttcat ctataaatat
     3781 tatacttgga gcttcttttc tagctttttc aaatagagtt ctaactcttt ttgctcctac
     3841 acctacatat ttttcaacga actctgaacc agtaacattg aaaaatgatg agtttgtttc
     3901 accggctaca gcagatgcaa gtagagtttt acctgttcct ggaggaccat aaaataatac
     3961 tcctttaggt atttttgcac ccattttttg atatttttgt ggtgatttca taaaatcgac
     4021 tatttcaaat agttcttctt ttacttcgtc taatccagca acgtctctaa atgtagtctt
     4081 tggttttgta gaaagtgaat catctgtttg attgtctctt tccatttttg tttgtgcaaa
     4141 gacaggagct ggattgttaa ttttcttaaa aaagttgttt agcattttca tattcacacc
     4201 tcaggtaatt aattattgtt aatcaatttt atattttcca tatttactat tttttattat
     4261 ttatttttca tggaaaaaat atacatgtta tctaaataaa aataaacttg taagatgtat
     4321 gaaaaagtga atttatgtat atagagattt ctatttgtgt attgaataaa gtaatacaaa
     4381 ttataaatta tgtattgatt atatgttgag taaagtatta taaagcatgt attgattaag
     4441 tattgaataa aatattacaa atttagatga aatgaaaatg ataaaaaatg ttgtactata
     4501 ctttacaaat catataaatg gaaaaattgt tatagcacaa aatagtgagt agcatattag
     4561 aatataatat agaatttgtt gtgcagtata gtagtatatt agaatataat gtagaatttg
     4621 ttgtgtagta tagtagtata ttagaatata atatagaatt tgttgtgtag tatagtggta
     4681 tattagaaga caatatagga tttgttatgt agaatatatg gagtcattta gaatatacat
     4741 attataaaag gattttgggt ataaatttaa aaattaatag caaaatgagt agtatagaca
     4801 tataattagt agtaggaagt aattaaattt ttcctaaacc aaaaaaaaca gttgcaaata
     4861 atttgattaa gtgatatagt aaataaggta agaataaaac aaagaagaag tccgcttctc
     4921 accttacgcc agaggttagt aaggtaaata taagttaaag cttaatttca atttaagctt
     4981 gtttaaattt ggtaacttat attgcggatg attacgtcat ccgcttttta ttttataaaa
     5041 attttggagg tgtcctacaa ttagcaaaga attagcaatc aatgaacaaa taaaagataa
     5101 ggaaataaga gtcctttcac aaacaggtga acaacttgga gtaatgccta ctaaagaagc
     5161 tcaagcaatg gctaatagca aaaacttaga tttggttcaa atctcgccaa atgcaaatcc
     5221 accagtatgt aagataatgg attatggaaa gtttagatat gagcaagcga gaaaagagaa
     5281 agaagctaag aaaaaacaaa aaactatagt tgtaaaggaa gtaagactta gaccaggtat
     5341 tgaacaaaat gatttaaata cgaaagctaa taatgctata aagttcctta aaaaagggga
     5401 taaagtcaaa gttgaactta gatttagagg aagagaatta ggacataaag acataggtaa
     5461 agaagttatg cttaagtttt tagatataat aaaagagttt ggagaaccaa caaaagcccc
     5521 tgcatttgag ggtaataata tggttgtaat tatagatcca aaaaaataga tgattaactg
     5581 agaggaggaa tttgtcatgc ctaaaatgaa aactcataga ggtgcagcaa agagattaaa
     5641 gaaaacagga actggaaagt taaagagagc taaagcttat aaaaaacata tattaactaa
     5701 aaaatctgca aaaactaaaa tgaacttaag aaaatcaact ttagtaagtg atggagatgc
     5761 taagagaata gcacaattat taccatacta ggatacagat tataaataat aactatagaa
     5821 gagggatata aaaggaggtc ataacgatgg caagagttaa aaaagctatg aacgctcgta
     5881 aaaagcataa gaaaatatta aaacttgcaa aaggattcag aggatctaga agcaaattat
     5941 atagaccagc aaatacattc gtaatgaaag cattaaaaaa tgcttatata ggaagaaagt
     6001 taaagaagag agacttcaga aaactttgga tacaaagaat aaatgcagct gctagaatga
     6061 atggaatatc ttattcaaga ttaatgaatg gattaaaatt atctggtatt gaagttaaca
     6121 gaaagatgtt atctgaaatg gctatacaag acccagaagg atttgcaaaa ttagcagaag
     6181 ttgctaaagc aaaattagct tagtattgaa tcatgagatg taaagtcttt gagttatgta
     6241 aataattcaa gggctttttt attttttgaa aaatacttat tagtgccata tacattcaat
     6301 tttataatga taaataaaat aagagataca atagagtttg ctcagattta atataaaaat
     6361 tattacaatt atctataata actatagaaa gttattttaa aaataacatt gatatatgta
     6421 taaagtttcc agctaatctt gaattaatat tatcaatatg ataatattat cttgttaaga
     6481 atatatgtag agggagagat aatatgaatg ataaactgat ggattgtttt gtaaatccaa
     6541 ttaaatgcaa gttattactt gaaatttttt caaataatag aacgacagct aaacaattag
     6601 gagaaaaatt tcaagatata tcaaaggcaa cactttatcg ttaccttaat aaaatgttga
     6661 aagatgatat tataaaagtt gttgaagaaa ataaaataag gggaactgtt gaaaaagtat
     6721 atgctatgaa gttagatata gctgatgatg ttaacaaaat tatagatgag aactctggag
     6781 aagaatatgc tcaactgttc actcaatata tgatgggatt tttaagagaa ttttatgaat
     6841 atgctgaaag aaaggaaaag gatatcctaa aagatggttc agttttttct gtctgtccag
     6901 tatatgctag tattgacgag atagttgaag cgagtaaaaa aattgctagt atactagaac
     6961 ctctgaaaaa taattcacat gaagaaggta gaagaatgca ttctatagga gtgataatta
     7021 ctccacctaa agaagatgaa ggtaaataaa tgataaaagt aacagtttga tttttatgac
     7081 aagctgttat tttaatgtat agtattttat ttataatatt ttattttaat gtatgcaata
     7141 aaatgtatat taagctggca tataaagtga tatgatatat ctaagggaaa attgtatata
     7201 tatgacatat ttaaaacaca ggggatgaaa attatgaggt taattacacg ttctgatttt
     7261 gacggattag tatgtggtgc attattaaaa gaagcgggga ttattgatag ttggaagttt
     7321 gctcatccta aggatttaca agatggattg gttgaggtaa cagaagatga ttgccttgca
     7381 aatgtaccat ttgttgaggg gtgtggtctt tggtttgacc atcactctag tgaatttgag
     7441 agaaataagc ttgaaggaag atataaaggt gaaagtcgta ttgctccttc ttgtgcgaga
     7501 attatttatg aatattacgg tggaaatgaa agattttctc actttaatga aatgttagaa
     7561 gcagtagaca aagttgattc tggaaatctc actatagaag aggtacaaaa tccatctggc
     7621 tggattttaa ttggttatct tatggaccca agaacaggat tgggacgttg gagaaatttt
     7681 actatatcta actaccaatt aatggaaaag ttgattgatg catgtagaac tttaagtaca
     7741 aaagaaattt tagaacttcc agatgtaaag gagcgtatag aagtttattt tgagcaaaca
     7801 aataaattta aagagatgat tgctaaatat acacgtgtag aaggagatgt tattatttct
     7861 gatttacgtg gggtagagcc aatttacaca gggaatcgtt ttatgattta cagtatgtat
     7921 ccagagcaaa atatttctgc ttggattgta aatggtaagg gcggaaaagg gtgttctgta
     7981 gctgttggat acagtatttt aaatagaact tcgagtgttg atgttggtag cctgatgctt
     8041 aaatatggtg gcggtggaca caaagctgtt ggaacttgcc aatttaatga tgaacaaata
     8101 gatgaattgc ttccaaagct acttgatgag cttgtaaaca ctaaaaacta gtggataaac
     8161 ttataaattt taaaaactac tacgaataaa atagaacttc attgtaccat ttcatttaat
     8221 gacttggagt gatacaatga agttttttaa tttttcatat caataactaa agaaataaac
     8281 tttagaccac atatacaatt aggttacatt ataccattag aacagatata taaaataaaa
     8341 aatcagtatt tttatagcag acaaattgac tgttattctt tttaaaagtc atatattagt
     8401 tgttgttaga tggcgaaaaa aattatagga aactattgaa taaatgaata ttgcctataa
     8461 tcatataaac tgctatagtt taaaataaag cagaacgtta aattttatta taatcttaaa
     8521 atttaagagg gggattttat gaaaataaaa tttgttatta tgacattagt gatattttta
     8581 atagcattac tagtgggatg tcaaagtaca aaagatacag aagttaaagt aaaagataca
     8641 gaaattaaaa tattagggac aggtgattta cattcaattt tgacagactc tatggtttca
     8701 tatgtaaatg aggaaaggga aaagaataaa aatctgatta tggtagatgc aggagatttc
     8761 tatggtacac aaagtagaga aatgtgggag tggtcttctg gaaaaaagtt aattaatatt
     8821 agaagagata gtagggcaga gtatgatgac ataattaaga atagtaaaga tgaagtacct
     8881 atagttaaag agatggcaaa gttgaaatat gatgctgtag ttttgggaga taatgaattt
     8941 gtttcaaatg ataagcaaag tcttgataaa ctagtttctg attttaaaaa taataatatg
     9001 ccattggtat cagcaaatat atatgaacaa tctggagaaa attatgtaca accatatgta
     9061 atgaaaaata ttaagacaga tgagggcaat gtaaaagttg gaatactagg tcttactata
     9121 aaagaagtag gggaaaccct tggtcttgaa gattttgaaa aggataaaaa agcaagagcg
     9181 ttaggagaac aagctgatta caaaggaaaa ttatatgcaa atgatttaat agaggatgca
     9241 aagaaatgga taaaagttat gaaaaaagaa agtccagaca taatagttgc agttgtacac
     9301 tcaggggaag agcataaaat ggatagaaat ccaggaaata gaataaaaga gttagcaact
     9361 acagtggatg gaatagatgt aattgtagct ggtcatactc atgaaaaaat agatgaacat
     9421 aaatataaaa ataaatctgg agatgaagta attgtcacac aaccaggttg gcatggagat
     9481 agagtgtcag agattaattt caaattaaat aaagaaaatg acaagtgggt tataaaggaa
     9541 aagtctagta aattaaaaat gattgattaa aaaatagcta cgcacacttg tgactttagt
//
LOCUS       DAFNNB010000146         9368 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0151, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000146 DAFNNB010000000
VERSION     DAFNNB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9368
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0151"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..702
                     /locus_tag="L3M60_002959"
     CDS             <1..702
                     /locus_tag="L3M60_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBN6227597.1"
                     /translation="FLPIVCKNLNKKTIIITIILVSLAFELTQYIFALGSSDIDDVIL
                     NSLGGIIGITIYINMNKLFPNDIKRFKVIIATSLILGVIGLGVISKNYHNLLTFKFRP
                     DKKISKILIEENREIIKDINKDTVDIVGTFESFKKGIITIKAGSNNKVKRPENLIDSD
                     GNIRIYLNENTKLVSYIITKESKMDIVKYEEFDVKNLDLLRRYDTINVWIDKENQPKD
                     KHGIMASKLLIGLYE"
     gene            817..1602
                     /locus_tag="L3M60_002960"
     CDS             817..1602
                     /locus_tag="L3M60_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease"
                     /protein_id="HBN6227598.1"
                     /translation="MEIIKYILYFFIYSFLGWTVESIGCSIASKRIINRGFLNGPICP
                     VYGFGAVIVISLLGRFNNVVIVFLLGMILTTILEYFTGFILETLFHAKWWDYSDRKFN
                     IKGRVCLKNAIYFGIMSVLIIRFIHPFIKDFVGIIPYRILISMSIITTLWAILDLIVT
                     IITLKKLDIKLNLLDDIITDLNDINVKLDKFDRGEIQALFKTINNDELEVREKINKIN
                     SKLDRIESNIILQKRVIKAFPHIKHKKQQEQLEHFKKIINEKR"
     gene            1894..3213
                     /locus_tag="L3M60_002961"
     CDS             1894..3213
                     /locus_tag="L3M60_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBN6227599.1"
                     /translation="MSEITSQMISTEEKKYVAKTQKIPYYPVAFKSGDGAMLYDYEGN
                     EYVDFLASAGSANVGHGNKEISQAVKEQMDDITQYTLAYFHSDPPVKLAEKLVEIAPG
                     DNDKKVLYSATGSACIDAAIKLARGYTGRTKIISMCESYHGSTYGAISISALSTNMRR
                     KMGPLLPEVYHFHYPDKNRTAKECLDEIEYAFAHYLPAEEVAAIFIEPIAGDAGIIVP
                     PVEWVQGLSKICKENGILLVSDEIQQGMGRTGKWFGIENFGVEADLIVLGKSVGGGLP
                     LGAVVGRTEIMQSLDAPAHLFTLAGNTTVCVAALKSIEIIEKENLLQKSIEMGDYIKA
                     GFEKLKEKYDIIGEIRGIGLSIGVDIVKGKGSNEKHPDATAKICYRCIQTGLIMIFLG
                     QSTLRVQPPLVITKEQVDKAMNIIDSAIDDYLNGRIGDEVYEVTQGW"
     gene            complement(3287..3721)
                     /locus_tag="L3M60_002962"
     CDS             complement(3287..3721)
                     /locus_tag="L3M60_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227600.1"
                     /translation="MKDNNNTNKTIQFGEKKASLENNIQKNKNIKLDNTHKVIPYPKN
                     RRKDDKKKGNKKRNTNTNTSKVNKNNYGHSFFNNKYKYKRKPYVIALYTVILILLAIL
                     IITKLTDSTNKGTYSRNTVSNTHKNDIRNVVFNESNNFLICG"
     gene            complement(3812..5188)
                     /gene="hydG"
                     /locus_tag="L3M60_002963"
     CDS             complement(3812..5188)
                     /gene="hydG"
                     /locus_tag="L3M60_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HBN6227601.1"
                     /translation="MFINHELINSLLEDAKNSTSDDIEKVLDKADRREKLSYKDIATL
                     LEVEDKKQLDRLFSIAGQIKNEIYGNRVVLFAPLYVSNYCVNECVYCGFSKCNKFKRK
                     KLTMEEIKEEVKILEKMGHKRLALEAGEDPKNCDINYILDCLDAIYSTYNENGNIRRV
                     NVNIAATSVDEYKLLKEKGIGTYILFQETYHKPTFIKMHGQSIKNDYYYHLTAFDRAM
                     EAGVDDVGAGVLFGLSDPKFEVLGLMMHNEHLEEKFGVGFHTISFPRLKKAEGMSLED
                     FPHLVSDDMFKKIVAITRLAVPFTGIIMSTRETAEMRNELLKYGVSQISAGSLTGVGG
                     YKAYEDGDNTEQFEVGDHRSPVEVLKELITDGYIPSYCTACYRKGRTGDRFMSLAKSR
                     QIHNVCTPNALTTLNEFLIDYGDEELKIMGKKLISEEIDKIEREDIRNIVSNNMTALE
                     RGERDLYL"
     gene            complement(5189..6274)
                     /gene="hydE"
                     /locus_tag="L3M60_002964"
     CDS             complement(5189..6274)
                     /gene="hydE"
                     /locus_tag="L3M60_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HBN6227602.1"
                     /translation="MTKNKIKYFIDKLYSINSLSFDELLYLIENIDNEEYNFEQNLRN
                     YLYKQASDMRNKHYGNRVYLRGLIELTNYCKNNCYYCGIQSSNKNIIRYRLSLDEILE
                     CCDIGYKIGYRTFVLQGGEDTYFTDDKICEIVSSIKNKYEDCAITLSLGEKSYDSYKK
                     YFLCGADRYLLRHETATDSHYKKLHPPNIELRTRKECLKNLKEIGYQVGAGFMVESPF
                     QNNEDLVRDLLYLKELNPHMVGIGPFIPHHDTIFKDYKHGDLEKTLLMLSITRLLLPK
                     VLLPATTALASINPSGRNAGLLAGCNVIMPNLSPQEFRKQYSLYDNKAFTGQEACEYH
                     QSLEENIKSLGLEVDYSRGDNIEWRRL"
     gene            complement(6310..7518)
                     /gene="hydF"
                     /locus_tag="L3M60_002965"
     CDS             complement(6310..7518)
                     /gene="hydF"
                     /locus_tag="L3M60_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HBN6227603.1"
                     /translation="MSLNSTPQSIRVHIGLFGKRNAGKSSIINAITNQSAAIVSDIAG
                     TTTDPVFRPMEILPIGPCVLIDTAGLDDVGKLGELRISKSLDVLEKTDIALLVVDCQI
                     GISQEDLSLIEKFNDKNIPHILILNKIDTIKNQSEILNLAKNKVKCPVVSVSSTDKIG
                     IENLKNEIIRVLPKDGTEFRLVSDLIEPNDLVVLVVPIDKAAPKGRLILPQQQVIRDI
                     LDNGAISIVTKENSLKETLSNLGKKPKLVITDSQVFPQVDKDTPKDIPLTSFSILFAR
                     QKGDLKELINGAYALETLKDGDKILMAEGCTHHRQTDDIGTVKIPNMIRKKTGKNITF
                     EFSSGVSFTEDINKYALVVHCGACMMNRAGMLSRIEKAKSFNVPIVNYGILIAYVKGI
                     LERSLELFNY"
     gene            complement(7533..7784)
                     /locus_tag="L3M60_002966"
     CDS             complement(7533..7784)
                     /locus_tag="L3M60_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HBN6227604.1"
                     /translation="MKKVAVISAILEEPKECQLKFNEVVSGFKGIIKGRMGIPCEEEG
                     VSIVCIAVVGEMDIINSLTGKLGNIPHVSVKTSISKKEI"
     gene            complement(8165..9205)
                     /locus_tag="L3M60_002967"
     CDS             complement(8165..9205)
                     /locus_tag="L3M60_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HBN6227605.1"
                     /translation="MNLNLTNTINLMKKYLDIPSPAGYTENAVLECKKDFEDLGLDTL
                     LTKKGALIATLPGEDDSNQITISAHMDTLGAMVKEISSDGTLKYHKVGGGCWAAIEGE
                     NCTVVTRKGKKIRGSVVFKYASTHIYGQTKAATERNEETMIIRLDEKVFTKQDVLDLG
                     ISVGDFVCLDHRFETTESGFIKSRYIDNKSAVAMVLEICRYFKENNLAPKHTTNFYIS
                     NYEEIGHGVSKCLPEKTKEFVAIDIAPTGPSQTSSEHGVTIVAKDNLSLYDFNLRNKL
                     VNVAEDNNLDYAVDVFTFYGSDASEAIRWGEDVQIACVGPGTNNSHHYERTHIEAIEN
                     TLKLLINYMLKE"
BASE COUNT         3335 a         1322 c         1310 g         3401 t
ORIGIN      
        1 tttttaccta tagtatgtaa aaatctaaat aaaaaaacta taattataac aattatactt
       61 gttagcttag catttgaatt aacacaatat atttttgctc ttggaagtag tgatatagat
      121 gatgttattt taaattcatt aggtggcata attggaatta ctatttacat aaacatgaat
      181 aaattatttc ctaatgatat taaaagattt aaggttataa tagctactag tttaatttta
      241 ggagttatag gattaggagt tatttctaaa aattatcata acttactaac ttttaaattt
      301 agacctgata aaaaaatatc aaaaatatta attgaagaaa atagggaaat aattaaggat
      361 ataaataaag atacagtaga catagttggg acatttgaga gctttaaaaa aggaataata
      421 actattaaag caggctctaa taacaaagta aagagaccag agaatttaat tgattctgat
      481 ggaaatataa gaatttattt aaatgaaaat acaaaattag tatcttatat aattaccaaa
      541 gaatcaaaaa tggatatagt taaatatgaa gaatttgatg tgaagaattt agatttatta
      601 aggagatatg atactattaa tgtttggatt gataaagaaa atcagccaaa ggataaacat
      661 ggtattatgg ctagtaaatt attaattgga ttgtatgaat aaaatattaa acaagaataa
      721 aagaataaca aatataaaaa acgaaaaaac tatttctata attattttta cgttgtgcat
      781 aaaataagaa cactggtgaa ggagtagaga taaattatgg agataataaa atatatcttg
      841 tactttttta tatattcatt tttaggatgg acagtagaaa gtataggatg ttctatagca
      901 agcaaaagaa taatcaacag aggtttttta aatggtccaa tatgccctgt ttatggtttt
      961 ggagcggtaa ttgtgatatc actattggga agatttaata atgtagtaat agtatttcta
     1021 ttaggaatga ttttaactac aatactagaa tattttacag gatttatttt agaaacctta
     1081 tttcatgcta aatggtggga ttattctgac agaaaattca atataaaggg tagagtatgt
     1141 ttaaaaaatg caatatactt tggtataatg tctgttttaa taataagatt tatacatcca
     1201 tttataaagg attttgtagg tataattcca tatagaatat taatttcaat gtctataatt
     1261 acaacacttt gggctatact tgatttgatt gttactatta taacacttaa aaaattagat
     1321 ataaaattaa acttactaga cgatataatt acagacctta atgatataaa tgtgaaattg
     1381 gataagtttg atagaggaga aatacaagct ttatttaaaa caataaataa cgacgaatta
     1441 gaagttagag aaaaaataaa caaaataaat agtaagctgg accgtataga gtctaatatt
     1501 attttacaaa aaagagttat aaaagctttt ccacatataa aacataaaaa acaacaagaa
     1561 caactagaac attttaaaaa aattattaat gagaaaaggt aattatacaa tttaatgaaa
     1621 aagaatagaa taatggtatt tataagatga ttttcagagg gctctataaa aatagaatag
     1681 agtcctttat tttttgaaaa ataatgttta tttgataaaa ataaaatata ttgtttctga
     1741 ataacaaata ttttgaaaat tataaaaaca tatattgaca atttgtgtca aaaatactaa
     1801 cattaagtat ataaagaatt ttatatgaga atatctttat aaatcgcaat aattttaaaa
     1861 cataaattta tttaaagagg ggatgatttg ccaatgagcg agataactag tcaaatgata
     1921 agtacagaag aaaaaaaata tgttgctaaa actcaaaaga taccttatta tccagtagca
     1981 tttaaatcag gtgatggtgc aatgctatat gattatgaag gtaatgaata tgtagatttt
     2041 ttagcaagtg caggctctgc aaatgtaggt catggtaata aggaaatctc acaagcagtt
     2101 aaagaacaaa tggatgatat aacacaatat acacttgcat atttccatag tgatcctcct
     2161 gtaaaattag ctgagaagct tgtggaaata gcccctggag ataatgataa aaaagtacta
     2221 tacagtgcaa ctggttctgc ttgtatagat gctgctataa aattagcaag agggtatact
     2281 ggaagaacaa agataatatc tatgtgtgaa tcttatcatg gaagcactta tggagccata
     2341 tctatatctg ctttaagtac aaatatgaga agaaaaatgg gtccattgtt accagaagtt
     2401 tatcacttcc attatcctga taaaaataga acagctaaag aatgtttaga tgagatagaa
     2461 tatgcttttg cacactattt accagcagaa gaagtagctg caatatttat agaaccaatt
     2521 gctggagatg caggaataat agtgcctcca gtagagtggg tacaaggttt aagtaaaata
     2581 tgtaaagaaa atgggatatt acttgtaagt gatgaaatac aacaaggtat gggaagaaca
     2641 ggtaagtggt ttggtataga aaattttggt gttgaagcag accttatagt tcttggaaaa
     2701 tctgttggag gtggattacc tctaggagca gttgttggaa gaacagaaat aatgcaaagt
     2761 cttgatgcac cagcacattt atttacttta gctggaaata caacagtatg tgtggctgct
     2821 ttaaaatcta tagagataat tgaaaaagaa aacttacttc aaaagagtat agaaatggga
     2881 gattatatta aagcaggatt tgaaaaacta aaagaaaaat atgatataat tggagaaata
     2941 agaggtatag gattatctat aggagttgat atagttaaag gaaaaggttc taatgaaaaa
     3001 catccagatg ctacagctaa gatatgttat agatgtatac aaactggatt aataatgata
     3061 ttcttaggac aatctacttt aagagtgcaa cctcctctag taataactaa ggagcaagta
     3121 gataaagcaa tgaacatcat agattctgca atagatgatt atttaaatgg tagaattggt
     3181 gatgaagtat atgaagtaac tcaaggttgg taaaataaat attaaataaa ataagagtta
     3241 tctcaatttt agaaagtaat ttctgaaatg agataactct tttaagttac ccacaaataa
     3301 gaaaattgtt tgattcatta aagactacat ttcggatatc gtttttgtga gtattagaga
     3361 cagtattgcg agaataagta cctttattcg tactatctgt taatttagtt ataattaaga
     3421 tagctaaaag aataagaatt actgtgtata aagcaataac gtatggtttt cttttatatt
     3481 tgtatttatt attaaaaaaa gaatgaccat aattattttt atttacttta gatgtatttg
     3541 tatttgtatt ccttttcttg ttacctttct ttttatcatc ctttcttcta tttttagggt
     3601 aaggaatgac tttgtgtgta ttatcaagtt tgatattttt atttttttga atattgtttt
     3661 ccaggctagc ctttttttct ccaaattgaa tagttttatt ggtattgttg ttatctttca
     3721 atgttaatct ccctccctta aactttccct ttagataata ttactactaa cctatggaat
     3781 atacaataaa taatatataa gtaataaaat tttataggta taaatctctt tctccacgct
     3841 ctaatgcagt catattgtta gatactatgt ttcttatatc ttctctttct atcttatcta
     3901 tttcttctga gataagtttt ttacccataa ttttaagttc ttcatctcca taatcgatta
     3961 gaaattcatt taatgtagta agagcatttg gtgtacatac attatgaatt tgtcgacttt
     4021 tagctaagct cataaatcta tcgccagttc tacccttgcg ataacaagct gtacaatagc
     4081 taggtatata tccatcagta attagttctt taagaacctc tacagggctt ctatggtctc
     4141 caacttcgaa ttgttctgta ttgtcaccat cttcatatgc tttatatccc ccaactcctg
     4201 ttagagaacc tgcactaatt tgtgatacac catattttag taactcattt ctcatttcag
     4261 cagtttctct agtggacatt atgattccag taaaaggaac agctaatcta gttatagcta
     4321 ctattttttt gaacatatca tcagaaacta agtgaggaaa atcttctaag ctcatacctt
     4381 cagctttttt caatctagga aaagatatag tatggaaacc tacaccaaat ttttcctcta
     4441 agtgttcgtt atgcatcata agccctagaa cttcaaattt agggtcagat aaaccaaata
     4501 aaactcctgc accaacatca tcaacgccag cttccatagc tctatcaaaa gctgttaaat
     4561 ggtaataata gtcatttttt atagattgac catgcatttt tataaatgtt ggtttgtgat
     4621 aagtttcttg gaataatata taagttccta ttcctttttc ttttagtaat ttatactcat
     4681 ctacacttgt agctgctatg ttaacgttta cccttcttat atttccattt tcgttatatg
     4741 ttgaataaat tgcatctaaa caatctaaga tataatttat atcacaattt ttagggtctt
     4801 caccagcttc tagagcaagt cttttatgac ccattttttc taatatttta acctcttcct
     4861 ttatctcttc catggtaagt ttttttcttt taaatttgtt gcatttgcta aaaccgcaat
     4921 aaacacattc atttacacag tagtttgata cataaagagg ggcaaataaa accactctat
     4981 taccatatat ttcatttttt atttgcccag ctatactaaa taatctatct agttgttttt
     5041 tatcttctac ttcaagaagt gtagctatat ctttataact aagtttttcc cttctatcag
     5101 ccttatcaag taccttttca atatcatcag aagttgaatt ttttgcatct tctaataaag
     5161 aattaataag ttcatggttt atgaacattt acaaacgcct ccattcaata ttatcaccac
     5221 gagaatagtc gacttcaagt cctaatgact tgatgttttc ttctaagctt tggtgatatt
     5281 cacaagcttc ttgaccagta aaggctttgt tatcgtaaag agaatattgt tttctaaatt
     5341 cttgaggaga aaggtttggc ataattacat tgcaaccagc taaaagacct gcatttctac
     5401 cactagggtt tatagatgcc aaggctgttg tagcaggtaa taaaactttt ggaagtagaa
     5461 gtcttgtaat tgaaagcata agtaatgtct tttctaaatc tccatgttta taatccttaa
     5521 atattgtatc atggtgtgga ataaaaggtc ctattccaac catatgagga tttaactctt
     5581 ttaggtataa taaatcacgc acaaggtcct cattgttctg aaatggtgac tcaaccataa
     5641 atccagcacc tacttgataa ccaatttcct taaggttttt taggcattct tttctagttc
     5701 taagctcaat gtttggtgga tgtaattttt tataatgact gtctgtagca gtttcatgtc
     5761 ttaaaagata tctatctgca ccacataaaa agtatttttt atacgaatca taactctttt
     5821 ctcctagtga tagagttatg gcacaatcct catatttatt ttttatactt gaaacaattt
     5881 cacatatttt atcatcagta aaatatgtat cctcaccacc ttgcaaaaca aatgttctat
     5941 atcctatttt ataacctata tcacaacatt caagtatttc atcaagtgaa agtctgtatc
     6001 ttatgatatt tttattagaa gattgtattc cacaatagta gcaattattt ttacaatagt
     6061 ttgtaagctc tataagtcct ctcaagtaga ctctatttcc gtaatgttta tttctcatgt
     6121 cgctagcttg tttataaaga tagtttctta aattttgttc gaagttatat tcttcattat
     6181 cgatattttc tattaaatat agtagttcat caaaacttaa tgaattaatg ctgtaaagtt
     6241 tatctataaa gtatttaatc ttatttttag tcatattcaa atatgtaatt attaaatata
     6301 gttaaatttt taataattga aaagttcaag tgatctttct aaaatacctt ttacatatgc
     6361 tattaaaata ccatagttta caatcggtac attgaaagat tttgcctttt ctattctaga
     6421 taacattcca gctctattca tcatacaagc tccacagtgg actacaaggg catatttatt
     6481 tatatcttca gtgaaggata ctccagaact aaattcaaaa gtaatatttt tgccagtttt
     6541 ctttctaatc atattaggaa tttttaccgt tccaatatca tcagtttgtc tatgatgggt
     6601 acatccttcg gccatcaaga ttttatcacc atcttttaaa gtttctaaag cataagctcc
     6661 attaatcaac tctttaaggt ctcctttttg tctagcaaat aatatagaaa aagatgtaag
     6721 aggaatatct tttggtgtat ctttatctac ttgtggaaat acttgagagt cagtaataac
     6781 aagtttcggt tttttgccta aattagatag agtttctttt aaactatttt cttttgtaac
     6841 tatagaaatt gcaccattat ccaaaatatc tcttataact tgttgttgag gaagtataag
     6901 tctacctttt ggagctgctt tatctattgg aacaactaaa acaactaaat catttggttc
     6961 aattaaatcc gataccaatc tgaactcagt gccatcttta ggcaaaaccc ttattatttc
     7021 attttttaaa ttttcaatcc caattttatc tgttgatgag acagaaacaa ctggacattt
     7081 aaccttattt ttagctaagt ttaaaatttc tgattgattc ttaatagtat caattttgtt
     7141 aagaattaaa atatgaggta tattcttatc attaaacttt tcaattagtg ataaatcttc
     7201 ttgtgatata ccaatttggc aatccactac tagtaaagct atatctgtct tttctaaaac
     7261 atctagtgat ttacttattc taagttcacc aagtttacca acatcatcaa gaccagcagt
     7321 atctataaga acacaaggtc ctataggaag gatttccatt ggtctaaata ctgggtctgt
     7381 agtagttcca gcaatatctg atactattgc agcgctttga tttgttatag catttattat
     7441 gctagattta cctgcatttc ttttaccaaa gagacctata tgcactctta ttgattgtgg
     7501 agtagaattt agactcattt gtactcacca ccttaaattt ctttttttga tatactagtt
     7561 tttacagata catgaggtat gttgcccaat ttaccagtca aactattaat aatatccatt
     7621 tcgccaacaa ctgctataca tacgattgat acaccttctt cttcgcaagg aatgcccatt
     7681 cttcccttaa taataccttt aaaacctgat acaacctcat tgaatttaag ctgacattct
     7741 tttggctctt ccaaaatagc acttataact gcaacctttt tcataaatat cctcctaact
     7801 cagaaaatat gtcttaaaca aatccaaaat caaaaaactt ttctttgtaa agaaaagttt
     7861 ctacaaaaga aaagttatta attacacaag tataatgtgc tatattaaac tatttcctta
     7921 cagattagat aactcttttc tggtagggtt agcttgtaaa aatttaattt aacaacaatt
     7981 aactgaagaa tatttttata caatttatat aaattataat aaaaattaac taaagaaaat
     8041 tgaagttact tatataatag tagtaaattg aaattaagtc tatagaaaaa ccaatttttg
     8101 tataaaaata aaaattaaag ctagttattt gttaagaatt gactttggta aaatatatat
     8161 ttaattattc tttaagcata taatttataa gtaattttag tgtattttca atagcttcta
     8221 tatgtgttct ttcataatgg tgactattgt ttgttccagg accaacacat gctatttgaa
     8281 catcttctcc ccaacgaata gcttctgaag catctgaacc atagaaagta aatacatcga
     8341 cagcgtagtc taaattattg tcctctgcaa cattcacaag cttgtttctt aagttaaaat
     8401 catataatga taaattatcc tttgcaacta tagttacacc atgttcacta gatgtctgac
     8461 ttggtccagt aggtgctata tctattgcta caaattcttt tgttttttca ggtaaacatt
     8521 tagaaacacc atgtccaatt tcttcataat tgcttatata aaagttagta gtatgcttag
     8581 gtgctaaatt attttcttta aaatatctac atatttcaag aaccattgct acagctgatt
     8641 tattatctat atatctagat tttataaaac cagattctgt agtctcaaat cgatggtcta
     8701 gacatacaaa atccccaaca cttattccta agtctaatac atcttgcttt gtaaatactt
     8761 tttcatcaag tctaataatc attgtttctt catttctttc agtagcggct ttggtttgac
     8821 catatatatg agtagaagca tacttaaata caacacttcc tctgattttt ttaccttttc
     8881 tagttacaac cgtacagttt tctccttcta tagcagccca acatccacca ccaactttat
     8941 ggtatttaag agtaccatca gaggatattt cttttaccat agctccaagt gtatccatat
     9001 gtgcagatat tgttatttga tttgaatcat cttcaccagg aagagtggct ataagtgcac
     9061 ctttttttgt aagaagagtg tcaagaccta aatcttcaaa atcttttttg cattctaaaa
     9121 ctgcattttc agtatatcca gctgggcttg gaatgtccaa atattttttc attaaattta
     9181 tagtattagt taaatttaaa ttcatattgt ttcctcctaa ataaatatat ttatacaaat
     9241 tataacataa atacaaatgt tctgaaaact aaaattaaaa gaataaaatc taaacatatt
     9301 taaaaaaaag tatataaaat aaatttaatt tatattatgt agatttaaag taaaaatagt
     9361 tttattta
//
LOCUS       DAFNNB010000147         9271 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0152, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000147 DAFNNB010000000
VERSION     DAFNNB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0152"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            38..1441
                     /locus_tag="L3M60_002968"
     CDS             38..1441
                     /locus_tag="L3M60_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN6227606.1"
                     /translation="MEKKKIGLWSLVFMNVSALYGIRWIAKSTASSFGLGLGAIPMWL
                     LFSFIYFVPGALICAELASTYQKGDGGLYDWVKEAFGEKYGFLVSWLNWTAKIFWYSS
                     FLTFLAVNVSYTIGNPALADNKMFVLILSLIIFWALSLIATKGISFTKIFTNTGALGS
                     TIPSAILIIFSFVAVFVLKKHDIASTYTIASMTPKINMDAFVAISAIMFGLAGAETAA
                     NFITEIDKPEKNFPKAILISAGIVASLYVLGSIAITMIIPPDQITASKGVLDALSAVC
                     ASLGIGSWLVQLIAFGIAFSVLGAIVLYIASPIKMLFGSVKKGIFPDSLVEVNEHKIP
                     SKAVILQAIIVTIILLVTTLMPSVDAIYNVLVTMTALTALFPYVLLYASYIKLRKERP
                     DEIRPYTMAKSTSTCLGLAKMVLVVTVVGILLSAAPVMPTFAENVIYEIEMIGGGLLV
                     ILSGLWLWNRYEKKTAK"
     misc_binding    1532..1772
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1815..2513
                     /locus_tag="L3M60_002969"
     CDS             1815..2513
                     /locus_tag="L3M60_002969"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBN6227607.1"
                     /translation="MNVIGIIGAMDEEVSILVDLMDIRETIKKASLEFYKGILEGKNV
                     VLVKCGIGKVNSALCAQILISEFKVDAIVNTGVAGALNEKLDVNDIVISTDAIQYDVD
                     TTAFGDPKGVIPRMKTSVFKADERLIDAAYKSSVEEVKTHKVLKGRVVTGDKFINSKE
                     LKEELVNDFGGYCGEMEGGAIAHVCYLNNTPFVIIRAMSDKADGSADVTYDVFVHDAA
                     NNSKDIVLNMLKSI"
     gene            2536..3537
                     /gene="trpS"
                     /locus_tag="L3M60_002970"
     CDS             2536..3537
                     /gene="trpS"
                     /locus_tag="L3M60_002970"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBN6227608.1"
                     /translation="MSEKKVIFSGAQPSGKMTLGNYLGAIKNWTELQDNYDCYYSIVD
                     LHAITVPQDPKVLRANAIELLAQYIACGLDPEKNTIFIQSHVKEHVELMWVLNTMTYM
                     GELSRMTQFKDKSQKSEANLNAGLFTYPVLMAADILLYQTDLVPVGDDQKQHLELARD
                     LANRFNNRFSPTFVVPEGYYPKGGARVMSLQEPTKKMSKSDSNENAFILLADDSDSIK
                     RKIKRSVTDSLGVVKYNDEQPGIKNLIDIYSNLSKKSVEEIVNMYEGKGYGIFKEDVA
                     EVVSEALRPIREKYVDLLNNKDYLEKIYSMGAEKAEKQARKTLRKVYKKVGFIERRY"
     gene            complement(4189..6819)
                     /gene="cdtB"
                     /locus_tag="L3M60_002971"
     CDS             complement(4189..6819)
                     /gene="cdtB"
                     /locus_tag="L3M60_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021426623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin binding subunit
                     CdtB"
                     /protein_id="HBN6227609.1"
                     /translation="MKVQMRNKKVLSFLTLTAIVSQALAYPVYAQTSTSSHSDNKKEI
                     INEDILTNNGLMGYYFTDEHFKDLKLMAPIKDGNLKFEEKKVDKLLNKDKSNVKSIRW
                     TGRIIPSKDGEYTLSTDRDDILMQVNNESTISNTLKVNMKKGKEYKFRIELQDKNLGS
                     IDNLSSPNLYWELDGIKKIIPAENLFLRDYSNIEKNDPFIPNNNFFDPRLMSDWEDED
                     LDTDNDNIPDSYERNGYTIKDLIAVKWEDSFAEQGYKKYVSNYLESNTAGDPYTDYEK
                     ASGSFDKAIKTEARDPLVAAYPIVGVGMEKLIISTNEHASTDQGKTVSRATTNSKTES
                     NTAGVSVNVGYQNGFTANVTTNYSHTTDNSTAVQDSNGESWNTGLSINKGESAYINAN
                     VRYYNTGTAPMYKVTPTTNLVLDGDTLSTIKAQENQIGNNLSPGDTYPKKGLSPLALN
                     TMDQFSSRLIPINYDQLKKLDAGKQIKLETTQVSGNFGTKNSSGQIVTEGNSWSDYIS
                     QIDSISASIILDTENESYERRVTAKNLQNPEDKTPELTIGEAIEKAFGATKKDGLLYF
                     NDIPIDESCVELIFDDNTANKIKDSLKTLSDKKIYNVKLERGMNILIKTPTYFTNFDD
                     YNNYPSTWSNVNTTNQDGLQGSANKLNGETKIKIPMSKLKPYKRYVFSGYSKDPLTSN
                     SIIVKIKAKEEKTDYLVPEQGYTKFSYEFETTEKDSSNIEITLIGSGTTYLDNLSITE
                     LNSTPEILNEPEVKIPTDQEIIDAHKIYSADLNFNPSTGNAYINGMYFTPTQTNKEAL
                     DYIQKYRVEATLQYSGFKDIGTKDKEMRNYLGDPNQPKTNYVNLRSYFTGGENIMTYK
                     KLRIYAITPDDRELLVLSVD"
     gene            complement(6872..8263)
                     /gene="cdtA"
                     /locus_tag="L3M60_002972"
     CDS             complement(6872..8263)
                     /gene="cdtA"
                     /locus_tag="L3M60_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylating binary toxin enzymatic subunit
                     CdtA"
                     /protein_id="HBN6227610.1"
                     /translation="MKKFRKHKSISNCISILLILYLTLGSLLPNNIYAQDLQSYSEKV
                     CNTTYKAPIERPEDFLKDKERAKEWERKEAERIEQKLERSEKEALESYKKDSVEINKY
                     SQTRNYFYDYQIEANSREKEYRELRNAISKNKIDKPMYVYYFESPEKFAFNKVIRTEN
                     QNEISLEKFNEFKETIQNKLFKQDGFKEISLYEPGKGDEEPTPLLMHLKLPRNTGMLP
                     YTNTNNVSTLIEQGYSIKIDKIVRIVIDGKHYIKAEASVVSSLDFKDDVSKGDSWGKA
                     NYNDWSNKLTPNELADVNDYMRGGYTAINNYLISNGPVNNPNPELDSKITNIENALKR
                     EPIPTNLTVYRRSGPQEFGLTLTSPEYDFNKPENIDAFKSKWEGQTLSYPNFISTSIG
                     SVNMSAFAKRKIVLRITIPKGSPGAYLSAIPGYAGEYEVLLNHGSKFKISKIDSYKDG
                     AITKLIVDATLIP"
     gene            complement(8653..9271)
                     /gene="cdtR"
                     /locus_tag="L3M60_002973"
                     /pseudo
     CDS             complement(8653..9271)
                     /gene="cdtR"
                     /locus_tag="L3M60_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906004.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family response regulator CdtR"
BASE COUNT         3137 a         1224 c         1378 g         3532 t
ORIGIN      
        1 tttaaaacaa ttaatggaga ggagaaatta aattaggatg gaaaagaaaa aaataggcct
       61 atggagtttg gtatttatga atgtatcagc tttgtatggt ataagatgga tagctaagtc
      121 tacagcatca agctttggtt taggattagg tgcaatacct atgtggttat tattctcatt
      181 tatatacttt gtaccaggag ctttaatttg tgctgaatta gcttctacat atcaaaaagg
      241 tgatggaggt ctttacgatt gggttaaaga agcctttgga gaaaagtatg gatttttagt
      301 ttcttggtta aactggacag ctaaaatatt ttggtattct tcatttttaa ctttcttagc
      361 agtaaatgtt tcttatacaa taggaaatcc agctcttgct gataacaaga tgtttgtact
      421 tatattatct ttaataatat tctgggcatt atctcttata gcaactaaag gaataagttt
      481 tacaaaaata tttactaata caggtgcatt aggttcaaca ataccaagtg ctatacttat
      541 aatattttca tttgtagcag tatttgtatt gaaaaaacat gatatagctt ctacatacac
      601 aatagcttca atgactccaa aaattaatat ggatgccttt gttgctatat cagctataat
      661 gtttggttta gcaggagcag agactgctgc taacttcata acagaaatag ataaacctga
      721 aaagaacttc cctaaggcaa tattaatatc agctggaata gtagcttcat tatatgtatt
      781 aggttcaatt gcaataacaa tgataatacc accagaccaa attactgctt ctaaaggtgt
      841 attagatgca ctttctgctg tttgtgcgtc tttaggaatt ggttcttggt tggttcaatt
      901 aatagctttt ggtatagctt tttcagtatt aggagcaata gttctttaca tagcgtcacc
      961 aataaaaatg ttatttggta gtgttaaaaa aggaatattt ccagatagtt tggtagaggt
     1021 aaatgaacac aagatacctt ctaaagcagt tatacttcaa gcaataatag ttactataat
     1081 actattggtg actacattaa tgccttctgt tgatgctata tacaatgttc ttgtaactat
     1141 gacagcatta actgcattat tcccatatgt attattatat gcttcttata ttaaactaag
     1201 aaaagagaga ccagatgaaa ttagacctta tactatggca aaatcaacaa gtacttgcct
     1261 aggtttagca aaaatggttt tagttgtaac tgtagttggt atattattat ctgcagcacc
     1321 agttatgcct acatttgctg aaaatgttat atatgagata gagatgattg gtggaggtct
     1381 tctagttata ttatctggtc tatggttatg gaatagatat gaaaagaaaa cagctaaata
     1441 aaatgtattt taaaaatttc ttttggatat tataatatat agttgacaat ttatagtgta
     1501 aggaatatta taaaataaag aaattaataa attaaatcct gagataagaa atagtaaata
     1561 aatatatttt tgcacagaga attaaggatg ctgagaactt aataaaaagt atttattgaa
     1621 tgggtctttg aggagtaaaa tgaaatgtaa tttaagatta cagagtattt gatatcgtgt
     1681 atgcacgtta tagcatatat taagatatat atattcaatt ttgagtgtat ataaatttag
     1741 gtggtaccac ggaaaatata tccgtcctat tgatttagga cggatttttt aatatagaaa
     1801 atttaggagg aaaaatgaac gtaataggaa ttataggtgc aatggatgaa gaagttagta
     1861 ttttagttga tttaatggat attagagaga ctataaaaaa agctagctta gaattttata
     1921 agggaatttt ggaaggtaag aatgtagtat tagtaaagtg tggtataggt aaggttaatt
     1981 ctgctctttg tgcccagata ctaataagcg aatttaaggt tgatgctatt gttaatacag
     2041 gtgttgcagg agctttaaat gaaaaacttg atgttaatga tatagtaata tcaactgatg
     2101 ctatacagta tgatgtagac actacagcat ttggagaccc taagggagtt ataccaagaa
     2161 tgaagacttc tgtatttaaa gcagatgaaa gacttataga tgctgcttac aaatcttctg
     2221 tggaagaagt aaagactcat aaagtattaa aaggaagagt agttacagga gataaattta
     2281 taaattctaa agaattaaaa gaagaactcg taaatgattt tggtggatat tgtggagaaa
     2341 tggaaggtgg agctatagct catgtatgct atttaaataa tactccattc gtaataataa
     2401 gagcaatgtc tgataaggca gatggaagtg ctgatgttac ttatgatgta tttgtacatg
     2461 atgctgctaa taattctaaa gacatagttt taaatatgtt aaaatcaata taaatatata
     2521 aggaggtaca aaaaaatgag tgaaaaaaaa gttatattta gtggagcaca gccatcaggc
     2581 aaaatgactt taggaaatta tttaggagct ataaaaaact ggactgaact tcaagataat
     2641 tatgattgct attatagtat agttgatttg catgcaataa cagtacctca ggaccctaaa
     2701 gttttaagag ctaatgctat agagttgctt gctcaatata tagcttgtgg attagatcca
     2761 gaaaaaaata ctatatttat acaatcacat gtcaaagagc atgttgaact tatgtgggta
     2821 ttaaatacta tgacttatat gggtgaatta agtagaatga ctcaattcaa ggataaatca
     2881 caaaagagtg aagcaaatct aaatgcaggt ttattcacat atccagtgct tatggctgct
     2941 gacatattgt tgtatcaaac tgacctagtt ccagttgggg atgaccaaaa gcaacattta
     3001 gagttagctc gtgatttagc aaataggttt aataatagat tttcacctac ttttgtagtt
     3061 ccagaaggat actacccaaa aggtggagca agagtgatga gtttgcaaga gccaactaaa
     3121 aaaatgagta aatctgattc aaatgaaaat gcatttatat tattagcaga tgattcagac
     3181 tctataaaga gaaagataaa aagaagtgtt actgattctt taggtgttgt aaaatacaat
     3241 gatgaacaac ctggtattaa aaatctaatt gatatatatt ctaatttaag taaaaagagt
     3301 gtagaagaga ttgtaaacat gtatgaagga aagggttatg gcatatttaa agaagatgtg
     3361 gctgaagtag tatcagaagc tttaagacct ataagagaaa aatatgttga ccttcttaat
     3421 aataaagatt atttagaaaa aatatatagt atgggagcag aaaaagctga aaaacaagct
     3481 agaaaaactc ttagaaaagt ttataaaaaa gtgggattta tagagagaag atactaatta
     3541 ttagctatcc ttacttgata aataaatttt aaagttatta aaaaagtata tgatatgaaa
     3601 gagagagtct caaagtaaac ttttgttcat gaggcactct tttttgtatg aaataaaata
     3661 tattttaatc atttcaacaa tgaaaaagag gattatttct attttgagac agtacctttt
     3721 gataataaaa aaagatgagc ttttgctcat ctcaaagggg ttagattatt cttactttgg
     3781 ggaaagtaga ataaaggtaa aagttaactg atgtcaactt tgtctttttt gcatatcact
     3841 actgagcata aaaaaatcag aaaaccatag ccttctgaaa caacttctaa catacattgt
     3901 attttatacc caatatgtaa tttatgagtt aattttaact cttataatga aaattgtcaa
     3961 taatatatca aaaaaaattt tttaaaattt atataattaa atgaatttat gtttatatat
     4021 ggatgatatg atatagataa ttatctttat aatacaatat agttctaaaa aagaatatat
     4081 tagaagaaga gaatatttaa ataaaaaata ccttataaag ttaatttttt agtactttat
     4141 aaggtaaata tatattgtat tgtggggaca aatttaaatc atttgatact aatcaacact
     4201 aagaactaat aactctctat catctggagt aattgcatat attcttaatt tcttgtatgt
     4261 cataatattt tctccacctg taaaataact cctaagattg acataattag ttttaggttg
     4321 atttggatct cctaaataat tacgcatttc tttatcttta gttccaatat ctttaaatcc
     4381 agaatattgt aaagtagctt caactctata tttttggata taatcgagag cttctttatt
     4441 agtttgtgtt ggtgtaaaat acataccatt tatataagca tttcctgtac ttggattaaa
     4501 atttaaatct gcagaatata ttttatgtgc atctattatt tcttggtcag ttggaatttt
     4561 aacttctggt tcattaagta tttcaggagt actattcagt tctgtaatag ataagttatc
     4621 taagtatgtt gtaccactac caattaatgt tatctctata ttagaagaat ctttttcagt
     4681 agtttcaaat tcataactaa actttgtata tccttgttct ggtaccaaat aatccgtttt
     4741 ttcttctttt gcttttatct ttacaattat tgaattagat gttaaaggat cctttgaata
     4801 tccactaaaa acataacgtt tataaggttt tagcttagac ataggtattt taatctttgt
     4861 ctcaccattt aatttatttg ctgagccttg taaaccatct tgattcgtag tattgacatt
     4921 actccatgta ctagggtaat tattgtaatc atcaaaatta gtaaagtaag ttggtgtttt
     4981 tataagtata ttcattcctc tttcaagttt aacattatat atctttttat cagacaaagt
     5041 ttttaaacta tctttaatct tattggctgt attatcatca aatatgagtt caacacaact
     5101 ttcatctatt ggtatatcat taaaatataa caaaccatct tttttagtag cgccaaaagc
     5161 tttttcaatt gcttctccaa ttgtaagttc aggtgtttta tcttctggat tctgtaaatt
     5221 tttagcagta actcttcttt cgtaagattc attctctgta tctaatataa tagatgcaga
     5281 aatactgtca atttgactta tatagtctga ccaactattt ccttctgtta ctatttgtcc
     5341 agaactattt tttgtaccaa aatttccact tacttgtgtt gtttctaatt taatttgctt
     5401 tccagcatct aattttttta attgatcata atttatagga atcagtctag agctaaattg
     5461 atccattgtg ttaagagcca gaggtgaaag cccttttttg ggataagtat ctccaggaga
     5521 tagattattg ccaatttgat tttcttgtgc tttgatagtt gataatgtat ctccatctaa
     5581 cactaaattt gttgttggtg tcactttgta cataggtgca gtacctgtgt tgtaatatct
     5641 aacatttgca tttatatatg cagattctcc tttgtttata cttaatccag tattccatga
     5701 ttctccatta ctatcttgaa cggcagttga attatctgtt gtatgggaat aatttgtagt
     5761 tacattagct gtgaatccat tttgatatcc tacattaaca gaaacaccag ctgtattaga
     5821 ttcagtttta ctgttagtag tagctctgga aacagtttta ccttgatcag tagaggcatg
     5881 ttcatttgta gatataatta atttttccat acctactcca acaattggat acgctgcaac
     5941 taacggatct cttgcttcgg tctttatagc cttgtcaaaa gaacctgaag ctttttcata
     6001 atctgtatat ggatctccag cagtatttga ctctaaataa tttgatacat atttcttata
     6061 gccttgttct gcaaagctat cttcccactt aactgcaatt aagtccttaa tagtatatcc
     6121 atttcgttca tatgaatctg gtatattatc attatctgta tccaaatctt cgtcttccca
     6181 atcagacatc aaccttgggt caaagaaatt gttatttggg ataaatggat catttttttc
     6241 tatattagaa taatctctta agaataaatt ttctgctggt ataattttct taataccatc
     6301 taattcccaa taaagattag gtgatgacaa attatctatt gaacctaaat ttttatcttg
     6361 tagctctatt ctaaatttat attctttacc ctttttcata ttaactttaa gtgtatttga
     6421 tatagtactc tcattattta cttgcattaa aatatcatct ctatcagttg ataatgtata
     6481 ttcaccatcc ttagaaggaa ttattcttcc tgtccatcgt atagatttta catttgattt
     6541 gtctttattt agaagtttat ctactttctt ttcttcaaat tttaaattac catcttttat
     6601 gggtgccatt aattttaaat ctttaaagtg ttcgtctgtg aaataatatc ccattaatcc
     6661 gttgtttgtg agtatgtctt catttataat ttctttttta ttatcagaat ggctacttgt
     6721 actagtttga gcatatacag gatatgctaa tgcttgacta actatggctg taagtgttaa
     6781 aaaacttaat acctttttat tcctcatttg tactttcatt atttctcctc ctttaatttg
     6841 aaatatatta aattatttat gtgaaaaaat attaaggtat caatgttgca tcaacaatta
     6901 attttgttat agcgccatct ttgtaagaat caattttact gattttaaat ttgcttccat
     6961 gatttaaaag tacttcatat tcacctgcat aacctggaat agctgataga taggctccag
     7021 gagaaccttt aggtatagtt atacgtagta ctatttttct tttagcaaat gcactcatat
     7081 tcacactacc aatactagta ctaataaagt ttggataaga cagtgtttgt ccttcccatt
     7141 ttgatttaaa agcatctata ttttctggtt tgttaaaatc atattcaggg gaagtaaggg
     7201 ttaaaccaaa ttcttgagga ccagatcttc tatatacagt taaattagtt ggaataggtt
     7261 cacgttttaa tgcattttca atgtttgtga ttttagaatc taattctggg ttagggttat
     7321 ttactggacc atttgatatt aaataattat taattgcagt atatcctcca cgcatataat
     7381 catttacatc agcaagttca ttaggtgtta atttattact ccaatcatta taatttgctt
     7441 taccccaaga gtccccctta cttacatcat ccttaaaatc aagactactt acaacagatg
     7501 cttctgcttt aatataatgt ttcccatcta taactatacg aacaatttta tctattttta
     7561 tactatatcc ttgctctatt aatgtactta cattgttagt atttgtatat ggtaacatac
     7621 cagtatttct aggtaatttt aagtgcataa gtaatggtgt aggttcttca tcaccttttc
     7681 caggttcata taaagaaatt tctttaaatc catcttgctt aaataatttg ttttgtatag
     7741 tttctttaaa ctcattaaat ttttctaatg aaatttcgtt ttgattttct gttcttatta
     7801 ctttattaaa tgcaaatttt tctggagatt caaaataata gacatacata ggtttatcta
     7861 ttttattttt tgatatagca tttcgaagtt ctctatattc tttttctcga gaatttgctt
     7921 ctatttgata atcataaaaa taatttcttg tctgagaata tttatttatt tctacagaat
     7981 cttttttata tgattctaat gcttcttttt cagatctttc aagtttttgc tctattcttt
     8041 ctgcttcttt tctttcccat tctttagccc tttctttatc tttaagaaaa tcttctggtc
     8101 tttctatagg agccttgtaa gtagtattgc aaactttttc actatagctt tgtaagtctt
     8161 gtgcataaat gttattaggt aacaaactac ctaaagttag atataatatc aacaatatag
     8221 atatacaatt actaatactt ttatgttttc taaatttttt catttattct ccctcccaat
     8281 attagtttaa ttaacttttg aattttaatt tatttagatt atataatcta aataagaatt
     8341 ttttatataa atttcataaa atatgtagct atatatcata aaaatatgta tttatatgat
     8401 atgtcagtac aaaataacaa ctgaatgttt taaagagact atttcatacc tttatgattt
     8461 aatgagctat cttgttgaat cataatctag gaacaattgt tacaatgtct aatagcaatt
     8521 gtatatgata tagtcagtat gtgttttttc acaaaacgat tatcatatag aggattctta
     8581 ggaactactt tttttgtata aaaaaagaga tggttcaaat ttttatcttg agacatcccc
     8641 tttttctatt tattatcttt taataatgtt ctttaaaata tttctatatg tattagatat
     8701 acgacatata tggccgttac tcattttaac attatttttc tttaggtcga tagaaacaat
     8761 atgatattta ttaactacaa aagatttatg tacacgaaca aagcatgcat ctaaatctga
     8821 taaagacttt aaacttttat agaattcaaa tattctagaa gtagtcacta attttatttt
     8881 atgtggaata gtgcttgttt caaaatatat aacttcatca aatgaaattt tccatagtga
     8941 ggaatctgag tagatagtaa aaaaattaga gcgataattt ttttctttaa tgtatctttt
     9001 atgagcaatt ttaatacaag cttttatctt actttcagca atttccatat catgttttag
     9061 tataaagtcc ataacttata acctataatc gaagatccct aaaatcattt ctatatgatt
     9121 tgtaagaaat atgattggac taatataatc tgattctcta attttttgtg ctatccataa
     9181 tccatcacat aattcattac ttatcttata ttttgcgtct acaactataa aatatattct
     9241 tactttattt ttttcagtaa gctcttttaa t
//
LOCUS       DAFNNB010000148         9160 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0153, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000148 DAFNNB010000000
VERSION     DAFNNB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0153"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            358..1563
                     /locus_tag="L3M60_002974"
     CDS             358..1563
                     /locus_tag="L3M60_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HBN6227611.1"
                     /translation="MSKKVLIMSASTGGGHNRAALAIKEELTSKTLDGEPIECEIIDS
                     LKLVNNTMDKIISRGYEKSAIYTPKAYGSVYRLSETNLLSKNEFKDNLLITFMAKKFK
                     KLIRSEKPDLIIGTHPFPMIALSTLKKNFNLHNNESNAYTDHFYKHYTNTINVPPLIS
                     VLTDYTTHSTWIQNEIDYYIVGHEYVKELLVFDGVEPSKIRTFGIPVEKSFLSHRDKD
                     TVLSELNLSPDKLTVLLMGGSFGAGNIKETLDELLDTDRDFQILVITGKNESLKEKIE
                     KKLISRYHDKNVCVLGYTDKMNDILASIDVLVSKPGGLTTTEALLKDVPMIVPYYIPG
                     QEEENLDFLSNCGAALRTTKKYNLSILLKVLIDDPSRLEMLKRNIKSIRKSNSAQNIA
                     NLILNIISG"
     gene            complement(1634..1855)
                     /locus_tag="L3M60_002975"
     CDS             complement(1634..1855)
                     /locus_tag="L3M60_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227612.1"
                     /translation="MKPIMNLEEHNAMKEKRVKKMKSKDKPKYDDFEDISFSNKKKTK
                     KNVRNKNAEFRNNYKNYQYDYEEDFYEPI"
     gene            complement(1997..2596)
                     /gene="recR"
                     /locus_tag="L3M60_002976"
     CDS             complement(1997..2596)
                     /gene="recR"
                     /locus_tag="L3M60_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBN6227613.1"
                     /translation="MQVYTGPITRLIEEFSKLPGVGRKTAQRLAFHIINMNTNDVEAL
                     SKAIIDAKREIRYCSICCNITDTDPCSMCSNKSRDSSVICVVEDPRDVAAMERTREFK
                     GQYHVLNGVISPMDGIGPDMLKIKELIQRLGNQEVKEIIMATNPTIEGEATAMYIARL
                     VKPMGIKVTRIAHGLPVGGDLEYADEVTISKALEGRREI"
     gene            complement(2609..2959)
                     /locus_tag="L3M60_002977"
     CDS             complement(2609..2959)
                     /locus_tag="L3M60_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009887752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBN6227614.1"
                     /translation="MAKKGFGGGMMPGGGNMNNLLKQAQKMQENMQKAQQELESKEVE
                     ASVGGGAVTVKVNGKKEVIDITIKPEVVDPDDIEMLQDLVLSAVNQALRNIDDIQASQ
                     MSKVTGGMNIPGLF"
     gene            complement(3018..4655)
                     /gene="dnaX"
                     /locus_tag="L3M60_002978"
     CDS             complement(3018..4655)
                     /gene="dnaX"
                     /locus_tag="L3M60_002978"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBN6227615.1"
                     /translation="MHKALYRAYRPQKFEDVIGQDHIIKTLKNQIYSDNIGHAYLFCG
                     TRGTGKTSTAKIFSRAVNCLNKINEEPCNECEICESVLKDNTMDVVEIDAASNNSVDD
                     IRELRESVKYSPANAKYKVYIIDEVHMLSQGAFNALLKTLEEPPSYVIFILATTEPHK
                     IPATILSRCQRYDFKRVTVKDMTLRMKKICEDEGIDIDDKALNLIARNSQGALRDALS
                     ILDQCMSFGESKIDYKDVVELMGSVNIEQLFELSQCIVEQDTKKSLEILNEFVLWGKD
                     IRNLINDLIDHFRNLMVCKVSSELDEIISLPEETIEQLKIQSKNIDINDLIRILNILS
                     ITQDDIKSSSNPRVLVEITIMKIAQPMFDESKEALIKRVENLEKMIELGNFKSEKIGN
                     NKEKEYEVDREIDVKQENVVYEDVKNEDVILIESSWKNILKQIKKDKKMPIYALLSEV
                     KSFNVYSNMLYVIFDDKFDFAKTRLSSQDTINYLEKTIRDVLNRSFNVKIVLTSEVKD
                     INLEVKEKKDIGEEILKNIVSEEILEIKDSIDENESK"
     gene            complement(4732..4996)
                     /gene="ffs"
                     /locus_tag="L3M60_002979"
     ncRNA           complement(4732..4996)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="L3M60_002979"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            complement(5034..5125)
                     /locus_tag="L3M60_002980"
     tRNA            complement(5034..5125)
                     /locus_tag="L3M60_002980"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5089..5091),aa:Ser,seq:gga)
     gene            complement(5126..5581)
                     /locus_tag="L3M60_002981"
     CDS             complement(5126..5581)
                     /locus_tag="L3M60_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HBN6227616.1"
                     /translation="MESSFYMKEALKEAYKAYNKKETPIGAIIVKDNQIIARAHNLTE
                     TLKDSTAHAEILAIKQASEKLGGWRLTDCDLYVTMEPCIMCSGAIVNSRIKKLIIGTR
                     HVKNSYIEKQHEFKLDYFNNNNVKVAFDVLQEECSIILQEFFKALRKRD"
     gene            complement(5903..7174)
                     /gene="serS"
                     /locus_tag="L3M60_002982"
     CDS             complement(5903..7174)
                     /gene="serS"
                     /locus_tag="L3M60_002982"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBN6227617.1"
                     /translation="MLDIKRIRENLDDIKKAMERRGEKEFDLDAVVELDNKRREILQE
                     VEVMKNELNVNSKNIPQLIKEGKDVTEEKARLKELSDKIKGIDEKVKEVEAKMEYALM
                     RIPNVPHPEVPQGETDEDNVQIRTWGEPTKFDFEHKAHWDIGTGLGILDFERAGKITG
                     SRFTLYRGLGARLERSLMNFFLNTHTAKHGYTEVLPPFMANRNSFIGTGQLPKFEEDM
                     FKIEGLEYFLIPTAEVPVTNIHANEILDVAELPIKYCAYTPCFRSEAGSAGRDTRGLV
                     RQHQFNKVELVKFVKPEDSYNELESLTHDAETMLQMLGLPYRVVKICTGDLGFTAAFK
                     YDLEVWMPSYNRYVEISSCSNFEDFQARRAGIRFKRDKKSKAEYVHTLNGSGLAIGRC
                     LAAILENYQQADGSVVVPEELRPYMGVDVIR"
     misc_binding    complement(7299..7505)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7633..8514)
                     /locus_tag="L3M60_002983"
     CDS             complement(7633..8514)
                     /locus_tag="L3M60_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HBN6227618.1"
                     /translation="MNFLSLAKSASTNSNITKRTLENMLDNFSDNIPNIIYAIIVFVI
                     GIYISKIVRRMVSKFLIKYGMSKGVNNFIVYGIYISMLSIISLISLGIIGIQTTSVVA
                     VLGAAGFSIGLAFKEILSNLGSGMIILFFKPFNIGDYIQGSGVEGTVSDIQIFSTVLK
                     TPDNKTIIIPNFQLTSNNIINYTHQNKRRIDFSYNISYDSDIDLVKSILNEIFINEKR
                     ILDDPRPIIGLNSIGNNTMQIVARPWVKTDDYWDVYFDVMEKVKNKFDENNIKVPFVP
                     SSLLFNNMSNLNTMDKR"
     gene            complement(8526..9035)
                     /locus_tag="L3M60_002984"
     CDS             complement(8526..9035)
                     /locus_tag="L3M60_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HBN6227619.1"
                     /translation="MNSNERRSKLIDILKESKHPVKGGTLAELLNVSRQVIVQDIAII
                     RARGFEIIATPQGYIIYNQPYFTKQIKCKNHKDSKEIYDELKIIVDLGGIIKDVIVNH
                     PTYGQITAELNIYSKMDIDNFMKKVETNEFKQLSVLTECSHIHTIEAIKEETIEAIVK
                     ELKDNGILS"
BASE COUNT         2944 a         1604 c          970 g         3642 t
ORIGIN      
        1 catctttatc ctgtctagtc aatataattt tttaattttt ttatttatca tatattcttt
       61 atatttaatt tccaaaacct aatgctaaac aaatctgttt atttcataaa actaatatat
      121 attctatgct ctattttcct ttttattcta tatttaaatt ttttatttac aataatattt
      181 ttaacataac atatttgata aaattaaatt tcatttcttt cacttgtaat ttttgtaatc
      241 ttgtattaga ttaatattaa aaaatagttt atcaataacc ttatttacaa agacagtttt
      301 atatatcaaa acataataca atataaatac tatgctacat tttaggagga ttttattatg
      361 agcaaaaaag tattaataat gtctgcttcc actggtggtg gacacaatag agctgctcta
      421 gctattaaag aagaacttac ttcaaaaaca cttgatggag agccaattga atgcgaaata
      481 atagatagtt tgaaattagt taataataca atggacaaga taatttcaag aggatacgaa
      541 aaatctgcta tatatactcc caaagcctat ggaagtgtat atagattatc tgaaacaaat
      601 cttttatcaa aaaatgaatt taaagataat ctactaataa cattcatggc taaaaaattt
      661 aaaaagctta ttagaagcga aaaaccagat ttgattatag gaacgcatcc ttttccaatg
      721 attgctctta gtacattgaa aaaaaacttt aatttacata ataatgaatc aaatgcttat
      781 acagatcatt tttataaaca ttatacaaat accattaatg ttcctccgct tatttctgta
      841 ttaactgatt atactactca ctctacatgg atacaaaatg aaattgatta ctatattgtt
      901 ggtcatgaat atgtaaaaga actattggtg tttgatggag ttgaaccgag taaaattaga
      961 acatttggaa tccctgttga aaaatctttt ctctctcaca gagataaaga tactgttctt
     1021 tctgagttga atctatctcc tgataaatta actgttttac ttatgggggg gagctttggg
     1081 gctggaaata taaaagaaac tcttgatgaa ttattggata ctgatagaga ctttcaaata
     1141 cttgtaatta ctggtaagaa tgaaagccta aaagaaaaaa tcgaaaaaaa acttatatct
     1201 cgttatcatg ataaaaatgt atgtgttctt ggatatactg ataagatgaa tgatatttta
     1261 gcttctatag atgtattagt aagcaaacct ggtggactta ctacaactga ggctctttta
     1321 aaagatgtac ctatgatagt tccttattat ataccaggtc aggaagaaga aaatcttgat
     1381 ttcttatcta attgtggtgc agcgcttaga actacaaaaa aatataactt gtcaatatta
     1441 ctaaaagttt taatagatga ccctagcaga ttagaaatgc taaaaagaaa tattaaatca
     1501 ataagaaagt caaattcagc ccaaaatata gctaatttaa ttttaaatat aatttctggt
     1561 tgataaaata aataaaatcc ctatagtata tatattatct attagggatt ttttattttc
     1621 taattattta atattaaatt ggttcataaa agtcttcttc gtaatcatac tgataatttt
     1681 tataattatt tctaaattca gcatttttat ttcgtacatt ttttttagtt tttttcttat
     1741 tagaaaaact tatatcctca aaatcatcat atttaggttt atcttttgat ttcatttttt
     1801 taactctttt ttctttcatt gcattatgtt cttctaagtt cataattggt ttcatgttgc
     1861 acccctatca aaatttttaa atgatttata caacaaatta tacaatacag aagaaattta
     1921 ttcaataaat ctaaatcaaa attaatttga atttaaccca aaaaaattac aaaaatcgag
     1981 gtattaacct cgatttttat atttctcttc tgccttctag tgcttttgat attgtgacct
     2041 catctgcata ctctaaatcg cctccaacag gaagaccatg agctatccga gtgactttga
     2101 ttcccattgg ttttactaac ctagcaatat acatggctgt agcttcacct tcaatagtag
     2161 gatttgttgc cattattatt tctttgactt cctgattgcc aagtctttgg ataagttcct
     2221 ttatttttaa catatctggt ccaattccat ccattggaga aattactcca tttaatacat
     2281 gatactggcc tttaaactct ctcgttctct ccatagctgc tacatctctt ggatcttcca
     2341 caacacatat aacacttgaa tctctacttt tatttgaaca catgctacat gggtctgtat
     2401 ctgttatatt acaacaaatt gagcaatatc ttatttctct ttttgcatct ataatcgctt
     2461 tagataaagc ttccacatca ttagtattca tgttaataat atgaaaagcc aatctttgag
     2521 cagtctttct tcctactcca ggaagctttg aaaattcctc tataagtctc gttattggac
     2581 ctgtataaac ttgcataaat accacctact agaataaccc aggtatattc atacctccag
     2641 ttaccttact catttgactt gcttgtatat catcaatatt tctcaatgct tgatttactg
     2701 cacttaaaac taaatcttgt agcatttcta tatcatctgg atcaactact tctggtttga
     2761 tagttatatc tataacttct tttttaccat taacttttac agtaactgct cctccaccaa
     2821 ctgaagcttc tacttcttta ctttctaact cttgttgagc tttttgcatg ttttcttgca
     2881 ttttttgagc ctgctttaac aagttgttca tatttccacc accaggcatc attccgcctc
     2941 caaaaccttt tttagccata aacactattc ctcctaaact tattttcatt ttattattat
     3001 atcataatag tattttatta tttactctca ttttcatcaa tactatcttt tatttctaat
     3061 atctcttcac tcactatgtt ctttaatatc tcttcaccaa tgtccttttt ctctttaact
     3121 tcaagattta tatctttaac ttctgatgtc agtactattt ttacattaaa acttctattt
     3181 aaaacatctc ttattgtttt ttctaaataa tttattgtat cttgagagct cagtctagtt
     3241 tttgcaaaat caaatttatc atcaaatata acatacaaca tattagaata tacattaaaa
     3301 ctttttactt cacttaagag tgcgtatatt ggcatttttt tatctttttt aatttgttta
     3361 agtatattct tccaagatga ctctataagt ataacgtcct cattttttac atcttcataa
     3421 actacatttt cttgttttac atctatttct ctatcaacct catactcttt ttctttatta
     3481 ttcccaattt tttcagactt aaagttcccc agttcaatca ttttttctag gttctcaact
     3541 ctttttataa gagcttcttt actttcatca aacattggtt gagcaatttt cattatggtt
     3601 atttccacta aaacccttgg atttgaagat gactttatat catcctgagt tattgataat
     3661 atatttaaaa tccttattaa atcatttata tctatatttt tagattgaat tttaagttgt
     3721 tctatagttt cttctggtaa tgaaattatt tcatccaatt ctgatgaaac cttacatacc
     3781 attaaatttc taaaatgatc aattaaatca tttataagat ttcttatatc tttgccccat
     3841 aagacaaatt catttaatat ctcaagagac tttttagtgt cctgctctac aatacattga
     3901 gacaattcaa ataattgttc tatatttacg cttcccatta attcaacaac atctttataa
     3961 tctatcttac tttctccaaa agacatacat tggtctaata tactaagtgc atctctaaga
     4021 gctccttgag aatttctagc tattaaatta agagctttat catctatatc aattccttca
     4081 tcttcacaaa tctttttcat tctaagggtc atatctttaa ctgtaactct tttaaaatcg
     4141 tatctttggc atcttgataa aatggttgca ggtattttgt gaggctctgt agttgctaat
     4201 ataaaaatta catatgatgg aggttcctct aaggtcttta ataatgcatt aaaagctcct
     4261 tgagacaaca tatgaacttc atctattata tatactttat atttagcatt ggccggagaa
     4321 tatttcacac tttctctaag ctctcttata tcatctacac tgttatttga ggctgcatcg
     4381 atttctacaa catccattgt attatctttt aatacagatt cgcatatctc acactcatta
     4441 cacggttctt catttatttt attcaaacag tttactgctc tcgaaaaaat ctttgcagta
     4501 gatgttttac cagtacctct tgttccacaa aacaaataag catgtcctat attatcacta
     4561 tatatttggt tttttaaagt cttaataata tgatcttgac caataacatc ttcaaacttt
     4621 tgtggtctat atgctctata taatgcttta tgcatctttt tacctctctt tagcaaaatt
     4681 aataaaaaaa tccctcatta tctaagggat tagtctttta tatattaaat gctgtacacc
     4741 atctatcgac ttaattccat aggcgttacc aaaacagtta gctcaactta ggcaccccca
     4801 cggcacacaa aagtgatcac ttaccgctgc ttccttccgg atctgacggg gttcatgagc
     4861 tttcattgcg caggacctaa gcatcaacac cacttattta gggcggccct acagaaaata
     4921 agcctttaga tggaattcaa tcctgctata gcggattgca ggttacaggg caccgctacc
     4981 tccccatcta gtacagcaaa attacaacca tatttaatta ctttcattgt ttctggcgga
     5041 gagagtggga tttgaaccca cgatacagtt ttacccatat actcgctttc caggcgagcg
     5101 ccttcgacca ctcgaccatc tctccttaat ctctttttct caatgcctta aaaaattctt
     5161 gcaatatgat cgagcattct tcttgcaaaa catcaaaagc tacttttaca ttattattat
     5221 taaaataatc taatttaaac tcatgctgtt tctctatata tgaatttttt acatgtcttg
     5281 tccctattat aagctttttt attctagaat taactattgc accactacac attatgcaag
     5341 gttccattgt aacatataaa tcacaatcag tcaacctcca accacctaat ttttctgatg
     5401 cttgttttat cgctaaaatc tctgcatgag ctgtagagtc ttttaacgtt tctgtaaggt
     5461 tatgagctct agctattatt tgattatctt taactataat agcacctatt ggtgtttctt
     5521 ttttattata tgctttatat gcttctttta atgcttcttt catataaaat gaactttcca
     5581 taattcctcc caaaacttat aacaaactta tcttatcata attcatttta taattcaact
     5641 taattttatt ttgtttattt aattatcact atatatagct atattttttt aatattagtt
     5701 ctaaaataat ttttagatat ttatacaaga gtattttata aaaatattta gtgttttatg
     5761 atgaaaattc tgtcatataa attaacataa aaaaactatg aaatttaata tttacaacaa
     5821 tttcatagtc ttttaattta ttgtttactc ataacatctt ttcttcatta aagcatattc
     5881 tatacataca attaatataa aattatctta taacatcaac acccatataa ggtcttaatt
     5941 cttctggaac tactacagaa ccatctgcct gctgataatt ttctaatata gcagccaaac
     6001 atcttcctat cgctaatcca gaaccattca atgtatgaac atattctgct tttgattttt
     6061 tatctctttt aaatcttatt cctgctcttc ttgcttggaa atcttcaaaa tttgaacaag
     6121 aagatatttc tacatatcta ttgtaacttg gcatccaaac ttctaaatcg tatttaaatg
     6181 cagctgtaaa tcccaaatcc cctgtacata tttttacaac tctatatgga agacctaaca
     6241 tttgcaacat agtttcagca tcatgagtta atgattctaa ttcattataa gaatcttctg
     6301 gttttacaaa tttaactaat tcaactttat taaattgatg ttgtctaact aaacctcttg
     6361 tatctcttcc tgctgaacct gcttcagatc taaaacaagg agtatatgca caatatttaa
     6421 ttggaagttc tgcaacatct aatatctcat tagcatgtat attagtaaca ggtacttcag
     6481 ctgttggtat taagaaatac tctaatcctt ctatcttgaa catatcttct tcaaatttag
     6541 gaagttgacc tgtacctata aaactatttc tattagccat aaatggaggt aaaacttctg
     6601 tatatccatg tttagctgta tgagtattta aaaagaaatt cattaaagat ctttctaatc
     6661 tagcgcctaa acctctatat aaagtaaatc ttgaaccagt tatttttcca gctctttcaa
     6721 aatccaatat acctagacca gtaccaatat cccaatgtgc tttgtgttcg aaatcaaatt
     6781 tagtaggctc tccccaagtt cttatttgaa cattatcttc atctgtttca ccttgtggta
     6841 cctctggatg aggaacattt ggtattctca tcaatgcgta ttccattttt gcttctactt
     6901 cttttacttt ctcatcaata ccttttattt tatctgataa ctcttttaac cttgcctttt
     6961 cttctgttac atcctttcct tcttttatta attgtggtat atttttagaa tttacattta
     7021 attcattttt cataacctca acttcttgaa gtatctctct tcttttatta tctaattcaa
     7081 ccacagcatc aaggtcaaat tctttttcgc ctcttctttc catagctttc ttgatatcat
     7141 ctaaattttc tcttattctt ttaatatcta acatattaat tcctcctaat aattttgata
     7201 atataaatta taattccaat taatttacaa ttattctagt tgtaaacctt taatttaaac
     7261 aaatttgaaa aataaaaaaa gacctccgtt taccctataa aagggacgaa aatcttttat
     7321 ccgcgttgcc accctaattg attatactaa aatgtataat cctctcagac ctataacgta
     7381 ggtaaaccgt ctagctttaa ctagaagctc tagagtagat tcaaaaacca ttgtaatgag
     7441 ttctcaccat ccactcattc tctgaataca atctgttttt tactattttc cttcacagct
     7501 ttttaactaa ttgttttatt tgataatcat tatattatat atattaacat ctttcaatac
     7561 tattattcaa atttttttag aattttcttt aattttttat attaaattca ctttttttac
     7621 ttaattaaaa ttctatcttt tatccattgt atttaaattg ctcatattat taaataataa
     7681 tgaggaagga acaaatggaa cttttatatt attctcatca aatttatttt tgactttttc
     7741 cattacatca aaataaacat cccagtaatc atcagtttta acccatggtc tagcaactat
     7801 ttgcatggta ttatttccta tagagtttaa acctataatt ggtcttgggt catccaatat
     7861 ccttttttca tttataaaaa tttcattcaa tatagactta actaaatcga tatcactatc
     7921 atatgaaata ttatatgaaa aatctatcct tcttttattt tgatgtgtgt aattaattat
     7981 attgttactt gtcaattgaa agtttggtat aattatggtt ttattatctg gagtcttaag
     8041 aacagtacta aatatttgta tgtcagaaac tgtaccttct acacctgaac cttgaatata
     8101 atcacctata ttaaatggct taaaaaacaa aataatcatg cccgaaccta aatttgataa
     8161 tatttcttta aatgctaaac ctatactaaa tccagcagct cctaacacag ctacaactga
     8221 tgtagtttgt attcctataa tccctaaact tatcaatgat ataatagata acatagatat
     8281 atatattcca tatacaatga aattatttac acctttactc attccatatt tgattagaaa
     8341 ctttgaaacc atccttctta ctattttaga tatataaata cctatcacaa atacaattat
     8401 tgcatatata atatttggta tattatcact aaaattatcg agcatatttt ctaaagttct
     8461 cttagttatg ttactatttg ttgaggcaga cttagctaat gataaaaaat tcatttaact
     8521 cctccttacg ataagattcc gttgtccttt aactctttta ctatagcttc tatagtttcc
     8581 tcttttattg cctcaattgt atgaatatgg ctacattctg tcagaaccga taattgttta
     8641 aattcattag tctcaacttt cttcataaag ttgtctatat ccattttaga ataaatatta
     8701 agttctgctg ttatctgacc atatgttgga tgatttacga tgacatcttt tataatacca
     8761 cctaaatcta caattatctt taattcatca taaatttctt tagaatcttt atggttttta
     8821 cattttattt gttttgtaaa atacggttga ttatatatta tatatccttg aggtgtagct
     8881 attatttcaa atcctctagc tcttataatg gctatatctt gtactattac ttgtctactt
     8941 acatttaata gttcagctag tgttccacct tttacaggat gcttagattc tttcaaaata
     9001 tctattaatt tacttcttct ttcattagaa ttcatattac accttctttc caaatcaaaa
     9061 actttaccta tattatataa ttctatattt tatcttatta taaatcaata ttgactttat
     9121 attctattta atcttaattt ttataataaa tgaaaaaaga
//
LOCUS       DAFNNB010000149         9145 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0154, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000149 DAFNNB010000000
VERSION     DAFNNB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0154"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            314..1690
                     /locus_tag="L3M60_002985"
     CDS             314..1690
                     /locus_tag="L3M60_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HBN6227620.1"
                     /translation="MKTIVRRLILYADGMRPTQIMVSGFAAIIVIGALLLTLPIASQS
                     GESIGLLNALFTATSAVCVTGLVMVDTATYWSLFGQIVIITLIQIGGLGFMTVATMFS
                     LMARKKIQLRERLLIQESLNQADLSGLVRLTRFVLIITITIEGIGALVLSTVFIPQFG
                     LSRGIWYSVFHAISAFCNAGFDLMGSVSGPFTSLNSYVNNFTVSMTVCALIVLGGLGF
                     PVVLDIVGKRRFSKLNVHSKVVLFSTATLILVGALFIFLIEFNQKATMADLPLKGKVL
                     SAIFQSVTARTAGFNTLDLATLRESSVFVMIILMFIGASPASTGGGIKTTTLAVLIIT
                     VRSFLSGKSDIEAFERRLAPATIKKSLGIFVISISAVIFGTLIISITQPNFTLVQSAF
                     EVTSALATVGSSLAGTPNLNALGKIIIIIFMFMGRVGSLTLFMAILSGGRRKSQPIRY
                     AEGKIMVG"
     gene            1990..2661
                     /locus_tag="L3M60_002986"
     CDS             1990..2661
                     /locus_tag="L3M60_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HBN6227621.1"
                     /translation="MKQYIVIGCGRFGSSVASTMHLLGHQVMAIDKNEDSVQSISDKV
                     THSLIVDVTDEQALRSLGLGNFDVAVVAIGSDIRASIMATLIAKEMGVELIICKAKDE
                     LQAKVLYKIGADRVVFPERDMGVRVAHNLVSDNILDHIELDPEYSIVEIVTPNSWVGK
                     TLIELELRARYEITVLAIKTGKNINVTPSPDEELTAGSILVIIGQNTSITAITSGNKG
                     IIRRR"
     gene            2848..3645
                     /locus_tag="L3M60_002987"
     CDS             2848..3645
                     /locus_tag="L3M60_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HBN6227622.1"
                     /translation="MITNINSKDNEKLKYTKALLKSKNRNKESKFIIEGYRIVVLALE
                     CMANLDYVFINEDFENKKEHVKLLEDLDKKNTKIYKTTNKNFKELVDTENTQGIIGVV
                     SFKKKKLSESINKKDKFVLILDRIQDPGNMGTIIRTADSAGVEAIIALKGCVDIYNPK
                     VIRSTMGSIFDMNIINASQDETVDMLKSLDFNIVSSYLNTDNFYDKIDYGSKVALVIG
                     NEANGINEELVSKSDILVKIPIYGKAESLNAAISSAILMYEIKKSLI"
     misc_binding    3906..4140
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            4190..5209
                     /gene="pheS"
                     /locus_tag="L3M60_002988"
     CDS             4190..5209
                     /gene="pheS"
                     /locus_tag="L3M60_002988"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBN6227623.1"
                     /translation="MQEKLLALREVALAEIKEAQSIESVESLRVKYLGKKGDITAILK
                     EMGKLSAEERPVVGKVANEVRENIELSINSKKEEINAIEKERKLKEEVIDVTQPGKVL
                     KVGKKHPITQIIDEVTDIFIGMGFSIAEGPEVETVENNFDALNAPKDHPSRDMSDTFY
                     INDGVLLRTQTSPVQVRTMRSQELPIKVIAPGRCFRSDSPDATHSPMFHQIEGLVVGK
                     DVTMAEFKGTMDIFVEKLFGSDIKTKFRPHNFPFTEPSAEVDVTCFKCGGKGCPMCKY
                     EGWIEILGSGMVHPNVLRNCGIDPEVYSGFAFGVGVERLAMLKYEIDDIRLLFENDMR
                     FLNQF"
     gene            5226..7619
                     /locus_tag="L3M60_002989"
     CDS             5226..7619
                     /locus_tag="L3M60_002989"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBN6227624.1"
                     /translation="MLVSLKWLRDYVDIDMDVKEFADKMTMTGTKVETIDYYGEEIEN
                     ILVGKILEIKQHPNADKLVVTKVDIGDKVVQIVTGATNISEGDYIPVAVNGSKLPGGV
                     EIKQTDFRGELSDGMMCSAAELGIDEHYIEEYKRGGIYILDHEDSYELGKDIKDVLGL
                     NDALIDFELTSNRPDCKCMLGIAREAAATIGTKVKYPEIEVKESDEEIDFKVEIDNPD
                     LCRRYVARMVTDVKIEPSPYWMQRRLTEAGVRPISNIVDITNFVMLELGQPLHAFDIN
                     QVETGRIVVRTAKDGEKLVTLDDVERTLDKDMLVITNGEKSLGLAGVMGGANSEITDD
                     TKTVLFESANFKPENIRMTAKKVGIRSEASSRNEKDLDPNLAEIAANRAAQLVEMLGA
                     GKVLKGVVDVYPNKPEPKKLVVNPQRINHLLGVDVPMEQFVGILESLEFKCNLVADDK
                     LEIDVPSFRTDMEQEADVWEEIARIYGFENIPSVQLEGNTTAGIKTSKQKFMDALKDN
                     STAVGLNEILTYSFVSPKGVDKIRVPEGNVKRNFVKLLNPLGEETSVMRTTLIPNMLD
                     VLSTNVSHKIEEVSAFECGHIFIPQDSELPKEENRMCVGMYGKDVDFFVLKGTIETIL
                     VNVGFKGYEIEPQDNNTTFHPGRCAKIVYNNKYVGTLGELHPDVIENYNLGQRVYVAE
                     IDIDFVFDNSDRTKNYVPLPKYPSTSRDIALIVKDDVFVKQIEDIIKENGQGLVESYK
                     LFDVYKGSQIEAGYKSIAYSITYRSKDKTLTDEEVAKVHDKILSELSEKLNANLRSN"
     gene            7644..8234
                     /gene="zapA"
                     /locus_tag="L3M60_002990"
     CDS             7644..8234
                     /gene="zapA"
                     /locus_tag="L3M60_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBN6227625.1"
                     /translation="MNKVMVKIHGVEYPMVGDKSEKFMISIADFVDKEMDKITRQNPK
                     LSLSVAAILTALNISDLLFECSDENEKLIKANEELSKKVGASNEELQLEIKSLKLTIA
                     EKEAENREAEAKMKELIEIIENKKQEIFELSNTTEGSRAELDAYKNKIEELSTQLEEA
                     NERATIAENLASEFQNKAYDLQLKCTGLNNDVKNVE"
     gene            8433..9116
                     /locus_tag="L3M60_002991"
     CDS             8433..9116
                     /locus_tag="L3M60_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HBN6227626.1"
                     /translation="MTSKSQMSESFFLCSLLAITGGFLDIYTYVVRGKVFANAQTGNI
                     VLFGLNIAEGNVKESFYYLIPILAFMLGVFIAEKIRKYFTNNKAKIHWRQVIIIIEMI
                     TILIVSFVPRGRFNMFVNVAISFICSMQVESFRKVNGNSFATTMCTGNLRSATETLFH
                     YIHTKNIFMIKKSLQYYAIIIFFIFGAILGTFFTKIFVEKSILICFFILAVVFGIMFI
                     NKDDSFNEN"
BASE COUNT         3536 a          995 c         1755 g         2859 t
ORIGIN      
        1 aatttaactt caaaaaagta tatgaaattt atactagtaa aatgataaat aaattctaag
       61 tacaaaatag taaaagttat gatagtttta gacctattat atttaaaaaa tatagatgtt
      121 tttggtgcaa tttaaattaa aagatatata caaaaattta aaatattcca aacagaaatt
      181 ataaaaatta ctatcattgt ctttatacaa tatttatact ataatagaat aataatttag
      241 aggaaagctc taaaaataaa caaattaagg gtcttaacag tagtgatttt atgttaagga
      301 ggtataaaga aagttgaaaa caatagttag aagacttatt ctctacgcag atggaatgcg
      361 tcctactcaa ataatggtat ctgggtttgc tgcgattatt gtaatcggag cattgttgtt
      421 aacactcccg atagcgtcgc agagtggaga gagtataggc ttattaaatg ctttatttac
      481 ggctacttca gcggtttgtg ttactggtct agttatggta gatacagcaa cttattggag
      541 tttatttgga cagatagtta taataacatt gatacaaatt ggtggtcttg gatttatgac
      601 cgtagccaca atgttttcac ttatggcacg aaaaaaaata caactaagag aaagattatt
      661 aatacaggag tcattaaatc aagctgattt atcggggctt gtaagactta caagatttgt
      721 attaataata accataacaa tagagggaat aggtgcacta gttttatcaa ctgtctttat
      781 tccacaattt ggtttgtcta gagggatatg gtatagtgta tttcacgcaa tatcagcgtt
      841 ttgtaatgcc ggatttgact tgatgggcag tgtaagtggt ccatttacat cgttaaattc
      901 ttatgtaaac aactttacag tatcaatgac tgtttgtgca cttatagtgc ttggagggct
      961 aggattccct gttgtacttg atattgtagg aaaaagaaga ttttcaaaat taaatgtgca
     1021 ttctaaggtt gtattgtttt caactgcgac acttatttta gtgggtgcat tatttatttt
     1081 tcttatagaa tttaatcaaa aagctactat ggcggacttg cctttaaagg gaaaagtact
     1141 atcagcaata tttcaatcag taacagcaag aactgctggt tttaatacgc ttgatttagc
     1201 cactttacga gaaagtagtg tatttgtaat gataattttg atgtttattg gagcatcacc
     1261 tgcatcaact ggtggaggta taaaaacaac tacactagct gttttaatca ttacagttag
     1321 aagttttcta tcaggaaagt cagatataga agcttttgaa agaagattag caccagcaac
     1381 tataaaaaaa tctctgggta tatttgtaat tagtatatca gcagttattt ttggaacatt
     1441 gattatttca ataactcaac ctaattttac attagttcaa tcggcttttg aggttacatc
     1501 agcacttgct acagttggtt caagtttagc tggaactcca aacttaaatg ctttaggtaa
     1561 gattataata ataatcttta tgtttatggg tagagttggt tctcttactt tatttatggc
     1621 tatactttct ggtggtagaa gaaagagcca accaatcaga tatgcagaag gtaaaattat
     1681 ggttggttaa tttaccaagg agtagtataa atttataatg ggtttagagt agtaatgtaa
     1741 atctgttgat aaaataaatg agcctagatg taattatatg aagaaataaa atcgactaat
     1801 gagcaaaatt aattaatgaa ataaaaaata gtgaaataaa aaatagtgaa ataaaataca
     1861 aataaaagat aaatcaaaat taaattaatc tatgtatata aaattataga ttgtatacaa
     1921 gttttgtatg cataaccgat taatttaata gatattatat aaattgtgta ttagggagga
     1981 agtttcgaga tgaagcaata tatagtcatt gggtgtggga gatttggaag ttcagttgcg
     2041 tctactatgc atcttttagg acatcaagta atggcaatag acaaaaatga agattcagtt
     2101 caaagtatat ctgacaaggt aacccattca cttatagtgg atgttactga tgagcaagcg
     2161 ttaaggtcat taggtttagg taactttgat gtagcagtag ttgcaatagg ttctgatata
     2221 agggcatcta taatggcgac tcttatagcc aaagaaatgg gtgtagagtt gataatatgt
     2281 aaggcaaagg atgaattaca agctaaagta ctttataaaa ttggcgcaga tagagttgta
     2341 tttccagaaa gagatatggg agtaagagtt gcacacaatt tagtttcaga taatatatta
     2401 gaccatattg aacttgaccc agagtattca attgttgaaa tcgtaactcc aaatagttgg
     2461 gttggcaaga cacttataga gcttgaatta agagctagat atgagataac tgtacttgct
     2521 ataaaaacag gtaaaaatat aaatgttaca ccttctccag atgaggaact tacagccgga
     2581 agtattctag ttataattgg tcaaaatact agtataacag ctataacatc tggaaataag
     2641 gggataatta gaagaagata atttattatt taatatatat ttaattgcaa tgaaagtaga
     2701 tagtatcata taattatgaa tagttatatg atactatttt tatttaatca aaagtaggat
     2761 ttagatgtaa gattaggtaa agagtatttt aagttaaaaa aggagtaagg aggttgtgct
     2821 aatatagaaa tttagcattt agataaaatg attacgaata taaacagtaa ggataatgaa
     2881 aaattaaagt atacgaaagc actgttaaaa tcaaaaaaca ggaataaaga atcaaagttc
     2941 ataatagaag gttacagaat agtagtactt gcacttgaat gtatggcaaa tcttgattat
     3001 gtatttatca atgaagattt tgaaaataag aaagaacatg taaaactatt agaagattta
     3061 gataaaaaaa acacaaagat atataagact actaataaaa actttaaaga attagtggat
     3121 acagaaaata ctcaaggtat aataggtgta gtttcattta agaaaaaaaa attaagtgaa
     3181 agtataaata aaaaagataa atttgtattg attttagaca gaatacaaga cccaggaaat
     3241 atggggacta taataaggac tgctgattct gctggggtag aggccataat agcactaaag
     3301 ggatgtgtcg atatatacaa cccaaaagta attaggtcta ctatgggttc tatttttgat
     3361 atgaatataa ttaatgcttc acaagatgaa actgtggaca tgcttaaatc attggatttt
     3421 aatatagttt caagttactt aaatacagat aatttttatg acaaaataga ttatggttca
     3481 aaggtagcat tggtgatagg aaatgaagca aatggaataa atgaagaact tgtatcaaag
     3541 tctgatattt tggttaagat acctatatat ggtaaagctg agtcattaaa tgctgcgata
     3601 agctctgcta tactgatgta tgaaataaaa aaatccttaa tttaatgtat tgtaataaat
     3661 ataatgtcat gttataatct tgaattaaat agtagagtat caaaaatagt caatatatat
     3721 taaaaaaata gttcaattaa ttatatatta gatgtgtatc atataaaaaa tgattgaatc
     3781 aattatatat tagatgtata ttatataaag aaataattga attaattata tattagaggt
     3841 atattaaaaa tagtcacata atttgaatgg aaatgatata atactaaaat aaacaatatc
     3901 atattgtaaa tgcaatgaaa gaggaaagta ttttgattaa acttagtaaa gagataaaca
     3961 cctaggctgg gagtgttttc taagaggtca cgagaagttc cctctggagt aacagagctg
     4021 aaattttata gtaggctttg acgtcaaaaa cgcgttaagt ttgttagagg tggtttgatg
     4081 atttttaaat tgttaaacta ctagggtggt accgcgaaac tatagccttt cgtccctaga
     4141 cagggattga agggcttttt ttatacaaaa aaacgaaagg gtgatatgtg tgcaagaaaa
     4201 attacttgct ttacgtgaag tagctttggc tgaaataaaa gaggcacaaa gcatagaaag
     4261 tgtagaaagt ttaagagtta agtacttagg aaaaaaaggt gatataactg ccatacttaa
     4321 agaaatgggt aaattatcag ccgaggaaag accagtagtt ggtaaggttg ccaatgaggt
     4381 aagagaaaat attgaactta gcataaattc taaaaaagaa gaaataaatg ctattgaaaa
     4441 agaaagaaaa ttaaaagaag aagtaataga tgttactcaa ccaggaaaag ttttaaaggt
     4501 tggaaagaaa catccaataa ctcaaattat agatgaagta acagatatat ttatcggaat
     4561 gggattctct atagcagaag gtccagaagt tgagactgtt gaaaacaact ttgatgcttt
     4621 aaatgctcct aaagaccatc catcaagaga tatgagcgac acattctata tcaatgatgg
     4681 ggtattactt agaactcaaa catctccagt tcaagtaaga actatgagaa gtcaagaatt
     4741 accaataaaa gtaattgcac caggtagatg ttttaggtca gattcaccag atgccacaca
     4801 ctcaccaatg ttccatcaga tagaagggct tgttgttgga aaagatgtta ctatggcaga
     4861 atttaaagga actatggata tcttcgttga aaaattgttt ggttctgata tcaaaactaa
     4921 gtttagacct cataacttcc catttacaga gccaagtgca gaggttgacg ttacttgttt
     4981 caaatgtggt ggtaaaggtt gcccaatgtg taaatatgaa ggttggatag aaatattagg
     5041 ttcaggtatg gttcatccaa acgtgcttag aaattgtgga atagacccag aagtttatag
     5101 cggatttgca tttggagttg gggttgaaag acttgcaatg cttaaatacg aaatagatga
     5161 tattagatta ttattcgaaa atgatatgag attcttaaat caattttaat taggagggta
     5221 tgtagatgtt agtatcttta aaatggctta gagactatgt tgatatagac atggatgtaa
     5281 aagagttcgc tgataaaatg acaatgacag gaactaaagt tgagacaata gattattatg
     5341 gtgaagaaat agaaaatata ttggttggaa agattttaga aataaagcaa catccaaatg
     5401 ctgataagtt ggttgtaact aaagtagata ttggagataa agttgttcaa atagttacag
     5461 gagctacaaa tatatcagaa ggagattata ttccagtagc tgtaaatggt tctaaattac
     5521 ctggaggagt ggaaatcaaa cagactgatt ttagaggtga attatcagat ggtatgatgt
     5581 gttcagcagc tgaactaggt atagatgaac attacattga agagtataaa agaggtggta
     5641 tatatatttt agaccacgaa gattcttatg aattaggaaa agatataaaa gatgttttag
     5701 gattaaatga cgctttaata gattttgaat taacttcaaa cagacctgat tgtaaatgta
     5761 tgctgggtat agctagagaa gcagctgcaa ctataggaac aaaagtaaaa tatcctgaaa
     5821 ttgaagtaaa agaaagtgat gaagagatag acttcaaagt tgagatagac aatccagatt
     5881 tatgtagaag atatgttgct agaatggtta cagatgtaaa aatagaacct tctccatatt
     5941 ggatgcaaag aagacttaca gaagcaggtg taagacctat aagtaacata gtcgatataa
     6001 caaacttcgt aatgttagag cttggtcaac cacttcatgc ttttgatata aatcaagtag
     6061 aaactggaag aatagtagta agaactgcta aagatggaga aaaacttgta acattagatg
     6121 atgttgagag aacattagac aaagatatgt tagttataac aaatggagaa aaatcacttg
     6181 gtttagctgg tgtaatgggt ggtgctaact cagaaataac tgatgataca aagactgtac
     6241 tttttgaaag tgccaatttc aaaccagaaa acataagaat gacagctaaa aaagttggta
     6301 ttaggtcaga agcatcttca agaaatgaaa aagacttaga ccctaatctt gcagagatag
     6361 cagcaaatag agctgcacaa cttgttgaaa tgttaggagc aggaaaagtt ttaaaaggtg
     6421 ttgtagatgt atatccaaat aaaccagaac ctaaaaaatt ggtagtaaat cctcaaagaa
     6481 ttaaccattt attaggtgta gatgtaccaa tggagcagtt tgtaggaatc ttagaatcat
     6541 tagaatttaa atgtaattta gtagctgatg ataaattaga aatagatgta ccaagcttta
     6601 gaacagatat ggaacaagaa gctgatgtat gggaagaaat agctagaatt tatggatttg
     6661 agaatatacc atctgtacaa ttagaaggaa atacgacagc aggaatcaaa acttcaaaac
     6721 aaaagtttat ggatgcttta aaagataatt caactgctgt aggactaaat gaaatattaa
     6781 catattcatt tgtaagccct aaaggagtag ataagataag agtaccagaa ggtaatgtga
     6841 agagaaattt tgttaagcta cttaatccat taggagaaga aacttctgta atgagaacta
     6901 ctttaatacc aaatatgtta gatgttttat caactaatgt atctcataaa atagaagaag
     6961 tatctgcatt tgagtgtgga catatattca tcccacaaga ttcagaatta cctaaggaag
     7021 aaaatagaat gtgtgtaggt atgtacggta aagatgttga tttctttgta ctaaaaggaa
     7081 ctatagaaac tatacttgta aatgtaggat ttaaaggtta tgaaattgaa cctcaagata
     7141 ataatactac cttccaccct ggtagatgtg ctaaaatagt gtataataat aaatatgtag
     7201 gtacattagg tgaattgcat ccagatgtta tagaaaacta caatctaggt caaagagttt
     7261 atgtcgctga aatagatatt gactttgtat ttgataattc tgatagaaca aagaattatg
     7321 taccattacc aaaatatcca tctacatcaa gagatatagc tcttattgtt aaagatgatg
     7381 tatttgtaaa acagatagaa gatataataa aggaaaatgg tcaaggatta gtagaaagct
     7441 acaagttatt tgatgtttac aaaggttctc aaatagaagc tggatacaag tcaattgcat
     7501 attcaataac ttatagaagt aaggataaaa ctctgactga tgaagaggta gctaaggtgc
     7561 atgataagat attaagtgaa ttaagcgaaa aattgaatgc taacttaaga tcaaattaac
     7621 aactaatgtc gggggaggtt aggatgaaca aagtaatggt taaaatccat ggcgttgaat
     7681 atccaatggt tggagacaaa tcagagaagt ttatgattag tattgctgat tttgtagaca
     7741 aagaaatgga taaaataaca cgtcaaaacc cgaaattgag cttatcagtt gctgcgatat
     7801 tgactgcatt aaatatatca gaccttttat ttgaatgttc agatgagaat gagaagttga
     7861 ttaaagctaa tgaagaattg agtaagaagg ttggagcatc taatgaagag cttcaattag
     7921 aaatcaagtc tttaaagctt acgatagcag aaaaagaagc agaaaatcgt gaagctgagg
     7981 ctaagatgaa agagttaatt gagattatag aaaataaaaa gcaagaaata ttcgaactta
     8041 gtaatacgac agaaggttct agggcggagt tagatgctta taaaaacaag attgaggagt
     8101 taagtactca acttgaagaa gcaaatgaaa gagctaccat agctgaaaat ttagcttctg
     8161 agtttcaaaa caaagcttat gatttacagc taaaatgtac agggttaaat aatgatgtaa
     8221 aaaacgtgga ataaattaaa agatttgctg tctcttgttt tttgagacag cttcttttaa
     8281 tgtataaata taaatgataa atataaataa atataaatga tgtataaata tagacaatat
     8341 ttaaatataa atgatttttt tatattaatg aaaaatagaa tctaaaatga ttttgatttt
     8401 gtatctatca ttagaaagtg agaggtttat atatgacaag caagagtcag atgtccgaat
     8461 cattcttttt atgttcttta ttggcaataa caggaggatt tttagatatt tatacttatg
     8521 tggtaagagg taaagtattt gctaatgcac aaactggaaa tatagttcta tttgggctta
     8581 atatagcaga aggtaatgta aaggaatcat tttattattt gattcctatt ttggcattta
     8641 tgttaggcgt atttatagct gagaagataa gaaagtattt tacaaacaat aaggcaaaga
     8701 ttcattggcg tcaggtcatc attataattg aaatgattac aatacttatt gtttcatttg
     8761 ttcctagagg aagatttaat atgtttgtaa atgttgccat ttcttttatt tgttcaatgc
     8821 aagtggagag ttttagaaag gtaaatggga atagttttgc aacgacaatg tgtactggaa
     8881 atttaagaag tgctacagaa accttatttc attatataca tactaagaat atttttatga
     8941 taaaaaagag tttacaatat tatgctatta ttatattctt tatatttgga gctattttag
     9001 gaactttttt tacaaaaatt ttcgtggaaa aatcaatact aatatgtttt tttattttgg
     9061 cagtggtatt tggaattatg tttatcaata aagatgattc atttaatgaa aattaacttt
     9121 ctgtaaattt acaaaaaaat gaagc
//
LOCUS       DAFNNB010000150         9139 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0155, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000150 DAFNNB010000000
VERSION     DAFNNB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9139
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0155"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(30..554)
                     /locus_tag="L3M60_002992"
     CDS             complement(30..554)
                     /locus_tag="L3M60_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBN6227627.1"
                     /translation="MKSIVVIYKSKYGSTKKYAKWLSELLSCDIFETKNINIEKVMKY
                     DTIILGGGLYACNIAGISFFKKNYKELQKKNLVVFGVGAAPYDDEVIKRLREVNFKDE
                     LNKVPCFYCRGAWDETKMSFLDYTLCSFFKKSIAKKERQGLKPVEAPLLSGEAFDWCN
                     KENLTPILDYIREL"
     gene            complement(603..1100)
                     /locus_tag="L3M60_002993"
     CDS             complement(603..1100)
                     /locus_tag="L3M60_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6227628.1"
                     /translation="MNVETLMKKFMVDDDSRKQAIFASIFVMQNKLQTTCDKLDPELT
                     MKQWLLLAIADSVDETLTLTKLGELMGCSRQNVKKLAIALEKKGFIQIEQNEKDSRAI
                     CLVTKGRVEEYSKKVRGIQEKVLQLLFKDFTEKEVEQLFFGIMKLSAGIENVKNYVEN
                     NDIEE"
     gene            complement(1333..2610)
                     /gene="hflX"
                     /locus_tag="L3M60_002994"
     CDS             complement(1333..2610)
                     /gene="hflX"
                     /locus_tag="L3M60_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBN6227629.1"
                     /translation="MDMAKKGITAGLNINNKSEDFNELMIELGNLCSACDIDVVGSIT
                     QNAKQVNRAFYIGTGKVEELLNLIKKESIEVVVFYNELSTSQLKNLEEKLNCEIIDRT
                     ALILDIFAQRAKTREAKLQVEVASLKYMLPRLVGSNENLGRQSGGVGTKNRGSGEKKL
                     ELDRRRIEEKITFLNRELDDLKFQRETQRSMRRKSNLPNVALVGYTNAGKSSIMNKLV
                     DIFKNSEEKKVFEKNMLFATLETSVRNIILANNKEFLLSDTVGFVSNLPHDLVKAFRS
                     TLEEACEADVLLHVIDISNPSYKNHIKVTEDTLKQIGADGIPMIHVYNKIDLIDVEVL
                     DEILDSIDKEGLFVSVKKDINIDKMIKCICDSIFKDYVRCKLLIPYDKGHVVSYFNEN
                     ASVISTEYREDGVILDVECSNIEYNKYKKYVLE"
     gene            complement(3339..5171)
                     /locus_tag="L3M60_002995"
     CDS             complement(3339..5171)
                     /locus_tag="L3M60_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227630.1"
                     /translation="MSRMGRGPMGKSMGAGQKANDFKGTMRKLIAYLSKFKISIILVI
                     VFAIGSASFSIVGPKILGKATTKIFEGLVSKVSGGNVGIDFNAIGKILMFLLCLYLIS
                     ALFSFIQGFIMSGISQKVSYNLRKEISEKLDRLPMKYFDTKTHGEILSRITNDIDTLN
                     QSLNQSMTQLITSVTTMIGVLIMMLSISGIMTLVAILILPISMFVISRIVKKSQKYFR
                     YQQEYLGNVNGQVEETYSGQTIVKAFNREDEVIEEFDKLNDSLYNSAWKSQFLSGIMQ
                     PLMMFIGNLGYVMVSILGGWLAIKKTIEVGDIQSFIQYVRNFTQPMTQIAQVANLLQS
                     TAAASERVFEFLEEEEEDQIVENAVSIDGLKGKIDFENVNFGYNPNKTIINDFSVNVK
                     PGQKVAIVGPTGAGKTTIVKLLMRFYDVNSGSILIDGHNIKDFNRSELREMFGMVLQD
                     TWLFSGSIMENIRYGKLNATDEEVIEAAKSAHVHRFIKTLPDGYNMKLNEEASNVSQG
                     QKQLLTIARAILADPKILILDEATSSVDTRTEVLIQKAMDNLMEGRTSFVIAHRLSTI
                     RDADMILVMNEGDIVEQGNHEELLKKGGFYANLYNSQFEEDEAM"
     gene            complement(5164..7410)
                     /locus_tag="L3M60_002996"
     CDS             complement(5164..7410)
                     /locus_tag="L3M60_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011009877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227631.1"
                     /translation="MFKLIKYLKKSALSIVIIVCLLVIQAVCDLSLPEYTSNIVNVGI
                     QQGGVENSVPSVIRESELDKITLFMDKSSKDKVLDNYTLLNEKDYVKYKDKYPGLKDE
                     SLYELNTKDKDTIDDLNIIFGKAILIVSGLEGDSKEVKAMKAQLMSKLPPQATQSGDV
                     DIFKLLSAMPKEQLDTLTKEMSKGFEGMPESMITQSSVSYVKSEYEKIGIDTEKTQNN
                     YILFTGAKMLGIAMVSMVATITVGFLAARVAASVGRNLRSGVFRKVMSFSNTEMNEFS
                     TASLITRSTNDIQQIQMLMVMLLRIVFYAPIMAIGGVIKVLNTNTSMAWIIAVAVVAI
                     LSLIVVLFSVVMPKFKLVQKLVDKLNLVSREIITGIPVIRAFSTEKHEEERFDKANKN
                     LTKTNLFVNRVMTCMMPAMMLIMNLITVLIVWRGAYSVDSGAMQVGDMMAFIQYTMQI
                     IMSFLMISMVSIMLPRASVSATRIDEVLVSDISIKDPEKSEEQEFKVDKKGLVEFRNV
                     SFMYPDAEEPILTNISFTAKPGETTAFIGSTGSGKSTLINLIPRFFDVTEGEILLGGV
                     NIKNVSQHDLREKIGYVPQKGVLFSGTIESNLKYGRKEATDEEMRTAAEIAQATEFID
                     SKPEAFKTEISQGGTNVSGGQKQRLSIARAIAKNPEVYIFDDSFSALDLKTDAALRKA
                     LKSKTAESTVLIVAQRISTILNAEQIIVLNEGEIVGIGTHKELLKNCEVYEQIALSQL
                     SKEELANE"
     gene            complement(7478..7939)
                     /locus_tag="L3M60_002997"
     CDS             complement(7478..7939)
                     /locus_tag="L3M60_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6227632.1"
                     /translation="MNSNSEEKLNDVFLQLMRIYFIRTHFYFDKLGLYHGQPQILFSL
                     KMKDGQSQKELADKRKVKASTTTVMIKRMEKAGLVERKQDEKDQRVSRIFLTDKGLEI
                     CNRVEEFNKELEREFFLGFSEEEKIVLRRLLMQVRDNLKAGCHKHEEEKYF"
     gene            complement(8244..8810)
                     /locus_tag="L3M60_002998"
     CDS             complement(8244..8810)
                     /locus_tag="L3M60_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HBN6227633.1"
                     /translation="MRKLEEILEYNKSFVENKEYEQYVTSKHPNKKIVVLSCMDTRLT
                     ELLPKAMNLKNGDVKLIKNAGATIMHPFGSIMRSVLVAIYEFDVDEVMVVGHHGCGMC
                     NVDTDKLLGKILDRGISNDVILTLRSAGIDTKQWLHGFDSAEESVKDSIDLIKGHPLI
                     PDGIIVHGLIISPETGKLDVVVNGYEDK"
BASE COUNT         2965 a         1640 c          883 g         3651 t
ORIGIN      
        1 gataataaat aattataaat atatacctct taaagttctc ttatataatc gagtattgga
       61 gttaaatttt ctttattaca ccaatcaaat gcttctccag acaataaagg tgcttctact
      121 ggtttaagac cttgacgttc tttttttgct atagattttt taaaaaaact acaaagagta
      181 taatctagaa aagacatttt tgtctcatcc catgccccac gacaataaaa gcaaggaact
      241 ttgtttaatt catccttaaa gtttacttct cttaatctct ttattacttc atcatcatat
      301 ggagcagctc ctacaccaaa tactactaga ttttttttct gtaattcttt ataatttttt
      361 ttaaagaaag aaattcctgc tatgttacaa gcataaagac ctcctcctaa aataatcgta
      421 tcatacttca taaccttttc tatattaata ttcttagttt caaaaatatc acaagacaat
      481 aattctgaaa gccacttagc atattttttt gttgaaccat atttactctt ataaatcact
      541 actatacttt tcatataaat tatctccttt ctgatgtcat acaaagttga ttcgacactt
      601 gcttattctt caatgtcatt attttctaca taatttttca cattttcaat tcctgcactt
      661 aatttcataa ttccaaaaaa taactgttcc acttctttct ctgtaaaatc tttaaataaa
      721 agctgtaata ctttttcttg aatacccctt acttttttag aatactcttc cactctacct
      781 tttgtcacaa gacagatagc tcgtgaatct ttttcatttt gctcaatttg tataaaaccc
      841 ttcttttcta aagcaatagc tagttttttt acattttgtc ttgagcaccc cattaattct
      901 ccaagtttag ttaatgtaag agtttcatct acagagtccg caatagctaa aagtaaccat
      961 tgtttcatag ttaattctgg gtctaattta tcacatgtag tttgaagttt attttgcatt
     1021 acaaatatac ttgcaaatat agcttgtttt ctactatcat catctaccat aaattttttc
     1081 attaaagttt ctacattcat tattatacac ttctctttat cttagatttt aattttttga
     1141 taactttaaa tagtaactag ttactttata ttatagtaat tggttactat tatgtcaaca
     1201 aatttatttt tatcataaat ggtaaaacgg catagcttgt ggctataccg tttcataaaa
     1261 tcatatttta caatcaattt ataattaatt ataaattaaa tatattttaa gctataataa
     1321 ttttttcaaa atttactcta aaacatactt tttatactta ttatattcta tattgctaca
     1381 ttctacatct aaaataactc catcttctct atattctgta cttataacgc tcgcattttc
     1441 gttaaagtaa gaaactacat gccctttatc atatggaatt aaaagtttac atcttacata
     1501 atctttaaat atactatcac aaatacattt tatcatttta tctatattta tatctttttt
     1561 aactgaaaca aataaacctt ctttatcgat actatctaaa atttcatcta atacttctac
     1621 atctattaaa tcaattttat tatacacatg tatcataggt ataccatctg caccgatttg
     1681 ttttaaagta tcttctgtca ctttaatatg atttttatag cttggatttg aaatatcaat
     1741 aacatgtagc agtacatctg cctcacatgc ttcttctaat gttgacctaa atgcttttac
     1801 taaatcatgt ggtaaattac ttacaaaccc aacagtatca gataataaaa attctttgtt
     1861 atttgcaaga attatatttc taacagaagt ttcaagtgta gcaaataaca tgtttttctc
     1921 aaatactttc ttttcttcag agtttttaaa tatatctact aatttgttca ttattgaaga
     1981 tttaccagca tttgtatacc caactaaagc tacatttgga agatttgatt ttcttctcat
     2041 acttctttga gtttctcttt ggaattttaa gtcatctaac tccctattta gaaatgttat
     2101 cttttcttct attcttctac ggtcaagttc taattttttc tcaccagagc ctctattttt
     2161 agtaccaact ccaccacttt gacgccctaa attttcattt gaacctacaa gtctaggtaa
     2221 catatatttt aaacttgcta cttctacttg aagtttagct tctctagttt ttgccctttg
     2281 tgcaaatata tcaagtatca atgctgttct atctataatt tcacaattga gcttttcttc
     2341 aagatttttt agttgtgatg tagaaagttc attgtaaaat acaactacct ctatgctttc
     2401 tttctttatt aagtttaata attcctcaac tttacctgtc ccaatgtaaa atgctctatt
     2461 aacctgcttt gcattttgag ttatactccc aacaacatca atatcacatg ctgaacataa
     2521 gtttccaagt tcaatcatta gttcattaaa gtcctcagat ttattattta tatttagtcc
     2581 tgctgttatt ccttttttcg ccatatccat attctcctta tctgctattt tttatttaat
     2641 ctatagcaca agattatggc tctataatta aattcatcaa gaattttatt ttttatctta
     2701 tccttaaatg acacttatta aattataaat tttaccatta taataaccat aatcgtgcat
     2761 tattttctca atcttatata aaaaatcact ttcttacacc tcctcctaaa tatattatgc
     2821 agttttttca ataaaaaaac tccttacaaa tctaagtaat acaaaacaag ctacttatat
     2881 cataagaagg attaaagttc taaatatata ttaaaaatac attagtttaa ttaacttgta
     2941 tgtaattatc attttatatt taaccctatt cttaaattta agtagttatt tacatgatta
     3001 ataaaagcaa taaaaatatc tacttcaata taatcatcaa ctattaaaat taaattttat
     3061 ttataataaa atttaaagaa tataattaaa tatacatttt gataatcacc tccaacattt
     3121 tccttactac atattataat aaaattaaaa cgttgtaaag acataaaatt gtgtagatgt
     3181 ctcaaaataa atagaatcct cttttttatt gctaaaatga taaaaataca ttttgtttca
     3241 tacataaaag agtgccttat gaaccaaaaa gttcatttta agacactctc atttatttta
     3301 tcttattaat taaacttcta tgctaagata gttttttgtt acattgcttc atcttcttca
     3361 aattgagaat tgtaaagatt agcataaaat ccacctttct ttagaagttc ctcatgattt
     3421 ccttgttcaa ctatatctcc ttcattcatt acaagaatca tatcagcatc acgtatagtt
     3481 gataatctgt gggctataac aaaacttgtt ctaccttcca taagattatc catagccttt
     3541 tgaatcaata cttctgttct agtatcaaca gaacttgtag cttcatctaa tattaaaatt
     3601 ttaggatcag ctaaaatagc acgagctata gtcaataatt gtttttgacc ttgagaaaca
     3661 ttacttgctt cttcatttaa tttcatatta tatccatctg gtaatgtctt aataaatcta
     3721 tgaacatgtg cagatttagc agcctctata acttcttcat ctgtagcatt caacttacca
     3781 tatcttatat tttccataat tgaaccacta aataaccatg tatcttgaag aaccatacca
     3841 aacatttctc taagctcact acgattaaaa tctttaatat tatgtccatc aatcagtatt
     3901 gaaccgctat taacatcata aaatctcatt aaaagtttaa ctattgtagt tttacctgct
     3961 cctgtaggtc caacaatagc aactttttga ccaggtttaa catttactga aaaatcattt
     4021 attatagtct tgtttgggtt atatccaaag ttaacatttt caaaatcaat tttacctttt
     4081 aaaccatcta ttgaaactgc attttcaaca atttggtctt cttcttcctc ttctaaaaac
     4141 tcaaatactc tttcagaagc tgctgctgtt gattgcaata aattggcaac ctgtgcaatc
     4201 tgagtcattg gctgtgtaaa gtttcttaca tattgtataa atgattgtat gtcaccaacc
     4261 tcaattgttt tcttaattgc aagccaacct cctaagattg ataccattac atatcccaag
     4321 tttccaataa acatcataag tggttgcatt atacctgata aaaattgaga cttccatgct
     4381 gaattataca ggctatcatt taatttatca aactcttcaa ttacctcatc ttcacgatta
     4441 aatgctttca ctattgtttg accactataa gtttcttcaa cttgaccatt tacatttcct
     4501 aaatattctt gttgatatct aaagtatttt tgtgatttct ttactattct tgaaattaca
     4561 aacattgata ttggtaatat taaaattgct accaatgtca taattccact tatagataac
     4621 atcattatca aaacacctat catagttgtt actgatgtta taagctgtgt catactttga
     4681 tttaaacttt gatttaaagt atctatatca ttcgtaattc tcgataatat ttcaccatgc
     4741 gtcttagtat caaaatactt cattggcaat ctatccaatt tttctgaaat ctcttttctt
     4801 aaattatagg atactttttg agaaattcca ctcattataa atccttgtat aaaagagaac
     4861 agtgcactta ttaaataaag gcaaagtaaa aacattaaga tttttccaat tgcattaaaa
     4921 tctataccta catttcctcc agaaacttta ctcactaatc cttcaaatat ctttgtagtt
     4981 gccttaccta aaatttttgg acctactata gaaaacgacg cactaccaat agcaaataca
     5041 attactaaaa ttattgatat tttaaattta gataagtatg caattagttt tctcatagtc
     5101 cctttaaagt catttgcttt ttgtcctgct cccatagatt ttcccatagg acctcttccc
     5161 attctactca ttagctagct cctcctttga aagttgtgat aaagctattt gctcataaac
     5221 ttcgcaattt tttagaagct ctttatgtgt tcctattcca actatttctc cctcatttaa
     5281 aacaattatc tgttctgcat ttaagatagt actaattctt tgtgcaacta ttaaaactgt
     5341 actttcagca gtttttgatt ttaaagcctt acgaagagct gcatcagttt ttaagtcaag
     5401 tgctgaaaaa ctatcatcaa atatataaac ttctggattt tttgctattg cccttgcaat
     5461 agaaagtctt tgtttttgac ctccagatac atttgttcca ccttgagaaa tttcagtttt
     5521 aaaagcttct ggtttagaat ctataaattc tgtagcttga gctatttcag ctgctgttct
     5581 catttcttca tctgttgctt cttttctacc atattttaaa ttggattcta tagtaccaga
     5641 aaacaatact cccttttgag gtacatatcc aattttttct cttaaatcat gctgagaaac
     5701 attttttata ttaactccac caagtaagat ttctccttca gtaacatcaa agaaacgagg
     5761 aattaaattt ataagagttg acttaccact accagtactt cctataaaag cagttgtttc
     5821 tcctggcttt gctgtaaaag atatatttgt taatataggt tcctctgcgt ctggatacat
     5881 gaatgaaaca tttctaaatt caacaagacc ctttttatct accttaaatt cttgttcctc
     5941 tgatttttct gggtctttta tgcttatatc tgacactagt acttcatcta tacgtgtagc
     6001 tgaaacggat gctcttggaa gcatgataga taccattgat atcattaaga atgacattat
     6061 tatttgcatt gtatattgta taaatgccat catatctcca acttgcattg ctccagaatc
     6121 aacactatat gcacctctcc aaactataag aactgtgata aggttcatta taagcatcat
     6181 agccggcatc atacatgtca taactctatt tacaaaaaga tttgttttag ttaaattttt
     6241 atttgcttta tcaaatcttt cttcttcatg tttttcagta ctaaaagctc tgataactgg
     6301 aataccagta attatttcac gacttactaa gtttaactta tctacaagct tttgaactag
     6361 tttaaactta ggcataacca ctgagaataa cactacaatt aatgataaaa tagcaactac
     6421 tgctactgct ataatccatg ccattgaagt atttgtgttt aaaactttta ttactccacc
     6481 tatagccatt ataggtgcat agaatactat tctaagcaac ataaccatca acatttgtat
     6541 ctgttgaata tcatttgtac tacgagtaat taatgaagct gtagaaaatt cattcatttc
     6601 agtatttgaa aatgacataa cttttctaaa gactccactt cttaaatttc tacctacact
     6661 tgctgctact cttgctgcta aaaatcctac tgttattgtg gctaccatac taaccattgc
     6721 tataccaagc attttagccc cagtaaataa tatataatta ttttgagttt tttctgtatc
     6781 aatacctatt ttctcatatt ctgactttac atatgatact gaactttgtg ttatcatact
     6841 ctctggcata ccttcaaagc ctttgctcat ctctttagtc aatgtgtcta actgttcctt
     6901 tggcattgct gatagcaatt taaatatatc aacatctcca ctttgagtag cttgtggtgg
     6961 aagtttactc attaactgag ctttcatagc ttttacctct ttactatcgc cctcaagccc
     7021 tgatactatc aaaatagcct taccaaaaat aatatttaaa tcatctatag tatctttgtc
     7081 tttagtattt aactcataaa gagattcatc ttttagacct gggtacttat ctttatactt
     7141 aacataatcc ttttcattca acaaagtata attatctagt actttgtcct tactgctttt
     7201 atccataaat aaagtaattt tatctaattc actctctcta atcacagatg gaacagagtt
     7261 ttctactcca ccttgttgta tacctacatt tactatattg gatgtgtatt ctggtaaaga
     7321 aaggtcacat accgcttgaa ttaccaatag gcagactatt attactattg ataatgccga
     7381 tttctttaaa tatttaatta atttaaacat acatttcttc ccttctgtaa tattaattag
     7441 tctaataaca ttaaattgaa aaatattatt ttaaacttta aaaatatttt tcttcttcat
     7501 gcttatgaca tcctgctttt aaattatctc gtacttgcat tagaagtctt ctaagtacta
     7561 ttttttcttc ttcactaaat cctaaaaaaa actctctttc caattccttg ttaaattctt
     7621 caactctatt acatatttct aaacctttgt ctgtcaaaaa aattctagat actctttggt
     7681 ccttttcatc ttgttttctt tccacaagtc cagctttttc catcctcttt atcataactg
     7741 tagttgtaga tgcttttact ttccttttat ctgctaattc tttttgactt tgcccatctt
     7801 tcatctttaa agaaaaaagt atttgaggtt gaccatgata taagcctaat ttatcaaaat
     7861 aaaaatgagt tcttataaaa tatattcgca ttaattgcaa gaaaacatca tttaattttt
     7921 cttcagaatt gctattcata cttgccctcc ttatttttta tattacttag tcggctaagc
     7981 attattttac tatactgcat gcattttttc aatatattta taaaaaattc atataaattt
     8041 catttattaa atattttcac atcatatagt tatactttta ataaataata gtaagatttt
     8101 taattagtca gctacgtata ttttttattg caaagcttaa aataaacctt ttcatatatt
     8161 ttcaaataaa aaagttgtcc caaaataaat tttgaaacaa catcttactc aacagtatct
     8221 tgattatata acactataat actctattta tcttcatatc catttacaac aacatctaat
     8281 tttccagttt ctggtgatat aattagacca tgtacaataa taccatctgg aattaaagga
     8341 tgccctttta ttaaatcaat actatctttt acagattctt ctgcagaatc gaatccatgt
     8401 aaccattgtt ttgtatcaat tccagcacta cgaagtgtaa gtattacatc attagatatt
     8461 cctctatcta aaattttacc aagtaactta tccgtatcaa cattacacat accacatcca
     8521 tgatgcccta caaccatgac ctcatctaca tcaaattcgt aaattgcaac taaaacactt
     8581 ctcataatac ttccaaaagg atgcattata gtagctcctg cattttttat tagtttcaca
     8641 tctccatttt ttaaattcat agccttaggc aaaagctcag ttaatcttgt atccatacaa
     8701 gataagacta ctattttttt atttggatgt tttgatgtca catattgttc atattctttg
     8761 ttctctacaa atgatttatt atactctaat atctcctcaa gttttctcat atgtaatatc
     8821 accctcttta aaaatttttt tattattata tcactattaa actacaatat atacatatat
     8881 aatttaattt atatttatta aataatttta taaaattatt actctactat aaatctcgta
     8941 tataacttgt atttataagt atttgtatat ttatttttta taatctaaac taatattgtt
     9001 cattttcatt atttttttaa ttatatattt tttaaaattt tattctaaat accttctcta
     9061 taattaattc tttatttcat ttctatataa ttatatttaa aaatatttac cttaccaata
     9121 taataattaa acttaaaat
//
LOCUS       DAFNNB010000151         8880 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0156, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000151 DAFNNB010000000
VERSION     DAFNNB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0156"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            70..1251
                     /locus_tag="L3M60_002999"
                     /pseudo
     CDS             70..1251
                     /locus_tag="L3M60_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901769.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            1367..3304
                     /gene="htpG"
                     /locus_tag="L3M60_003000"
     CDS             1367..3304
                     /gene="htpG"
                     /locus_tag="L3M60_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBN6227634.1"
                     /translation="MEFEKGSISIHTENIFPIIKKWLYSDKDIFIRELISNGCDAVSK
                     HKRLVSLGEISENKSSDYKIIVSVNKGEGTLKFIDNGIGMTEEEIKKYINQVAFSGAE
                     DFFNKYKDKMEESNDIIGHFGLGFYSAFMVSKKVQIDTLSYTEGATPVRWISEGGTEY
                     EISESDTRNDRGTTITLFIDDDSKEFLDEFTVRGIINKYCSFLPVEIYLEDVERLERE
                     AKEAEEKAKKQKEDGKEEVVDAKVIEPLNDTNPLWLKSPKDCTDEEYKEFYRKVFHVF
                     DEPLFWIHLNVDYPFNLKGILYFPKLKNEFELTEGKVKLYNNQVFVADNIKEVIPEFL
                     LLLKGVIDCPDLPLNVSRSFLQNDRDVSKISKHIIKKVADKLKSLCKNEREEYNKFWD
                     DIQIFIKYGCLKDESFYEKVKECILFKTIDDEYITLQDYLEKCKDKHENKVFYVSDKE
                     QQSQYIKLFKEYDLSAVVLNSSIDTHFISFMEYKENGVKFNRIDADLSDVLKDKNENK
                     DSEENKEEIAKIEGLFKETVGERVKNYSVEGLKNEDTPAMVLVSEQSIRMAEMQSRFA
                     GMDLGMNFEEEKTLVINENSPIIKKLVSLKDDEEKKDKVTLICNQIADLALLSNKELK
                     PDELDSFVQRSNKLMSLLIEL"
     gene            3683..4813
                     /locus_tag="L3M60_003001"
     CDS             3683..4813
                     /locus_tag="L3M60_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBN6227635.1"
                     /translation="MNFNYNLPVNILFGRGRINDVGKEVSKYGKKVLIVTGKNSTKKT
                     GLLDKTIDLLKDSKIEYEVFDRVEQNPLITTIYSGVEIIKATGCDCVLGLGGGSIMDA
                     AKSIAFSYKNPGDLNDYIFGIRQGEEALPIILVPTTSGTGSEGNCFAVLTNSETKDKK
                     SIKKNSMYAKSSIIDPELMVTMPKHIIASVGFDALAHNMEAYLSNGRNPIADVQAIYG
                     IELISENLVKVYNNVNDLEAWEKITLASTIGGMTIGTAGTALSHGMEHPVSGLRDVMH
                     GKGLAALTPVIIESSWDSDTERFGNISKILGGTCAEDCSDAIRNFLKKIDLDIKLSDL
                     GIEKSDIDWLTDNCMKISVANIKRHPKYFNKEEIKEMYRKSL"
     gene            5041..6063
                     /gene="splB"
                     /locus_tag="L3M60_003002"
     CDS             5041..6063
                     /gene="splB"
                     /locus_tag="L3M60_003002"
                     /EC_number="4.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore photoproduct lyase"
                     /protein_id="HBN6227636.1"
                     /translation="MFNPKRIIFEKGTLDTDVGKNIYNKFKDNKDVEIINISSNRIKE
                     HIPGENLYEQYRSGKQTMVVGIRKSLKFQTCKPSANYQLPLVSGCMGRCEYCYLNTQL
                     GDKPFIRVFVNVDEILEQTKKYIEDRKPQVTIFEGAATSDPVPVEPYTNSLKKTIEFF
                     GKEEKGRFRFVTKYSDVDSLLDAKHNNHTEIRFSINTPRIINEHEHYTASANKRIESA
                     VKLARAGYQIGFIIAPVFLYEGWKEEYKNLIRSIKEALPKDFDKKIIFEVISHRYTTK
                     AKNRILEIYPDTLLPMENEDRKFKYGQFGYGKYVYDKEQLDDMKEFFHKEINGVFKNS
                     LIKYII"
     gene            6230..7141
                     /locus_tag="L3M60_003003"
     CDS             6230..7141
                     /locus_tag="L3M60_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6227637.1"
                     /translation="MHAWESIQITLDLIEANLSEEISINELANKANLSPFYYQRLFKR
                     LVNKTVMEYIKLRRLAKASEYLAEHKNRILDVALKFGFASHETFTRSFKKAYGLTPEK
                     YRANPVKLNHFIKPELILNYAMVDEGVPLIADDIVIEVTRKTLSNPRTFVGIEMEVPI
                     CQLVGGETTGIAIVEELWMKLGSQRHNIPNQIPGGNEFAALYMGNAKEGNCMYMAGVE
                     VELGTSVEGYSTFELPAKEYLVCGFEAENFNELVNSAVFKADKFMERWMKKHNLTTAD
                     FAIEMYYPTTPEVAYLEHWIVPIPIEQ"
     gene            7156..7572
                     /locus_tag="L3M60_003004"
     CDS             7156..7572
                     /locus_tag="L3M60_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3788 domain-containing protein"
                     /protein_id="HBN6227638.1"
                     /translation="MNWQDTFFQDTKPTFEQINEFINNPLWDKLNKTLVSTYNVEPKL
                     EYSKCSMQRGWNVKYKKRGKSLCTLYPQEGYFKALVIVNESNRVEVDLFINTCCDYIK
                     QIYNEVKFFNGGKWLMIQVDSLLVLNDMLELIKFRI"
     gene            complement(7672..8064)
                     /locus_tag="L3M60_003005"
     CDS             complement(7672..8064)
                     /locus_tag="L3M60_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227639.1"
                     /translation="MGINSEKNITKNVEELTCPIRYALDIVGGKWKLPIICMLAVENP
                     IRYSSIKRKLDGITNTMLAQSLKDLESDGIVHRKQYNEIPPKVEYTLTSKGKSIVPIL
                     QQFANWGATNMQEKNKCGLNCKECRKTK"
     gene            8205..8600
                     /locus_tag="L3M60_003006"
     CDS             8205..8600
                     /locus_tag="L3M60_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HBN6227640.1"
                     /translation="MKALEFLNKAGVYYLATADENNQAHVRPLGFVMEYNGKLTFCTS
                     NQKDMFKQLIFNPKVELCCIDQNLNTLRILGEAVFVTSKETQAKALEIMPSLGQMYSV
                     GDGKFEIFSIDKAKVSCCSMSGKKIDVEL"
BASE COUNT         3625 a          900 c         1505 g         2850 t
ORIGIN      
        1 taagacatat tttagtacgc aaaattctgt gaagcaggaa ccctgtgact ttagtcatgg
       61 gaggttcagg aggctgtaga aagtttctat aagagttcac tttgtgaaaa aactgctctg
      121 tgtgaaaagt atataaacat gcttacatct gaggagtttt taattttctt tgaaaggtac
      181 aatcaactta taaggttaat aaacaagggt atgtataaag aagctataca agggtttaaa
      241 cttataactg agcaatactg tgatttaata gaaccttatg agttacttgc cttactgtat
      301 gacaaggaag aagaatatat aaaatttgat gaatgtttag aagtattgaa tactattgat
      361 aaggaaaact atttattaaa tcaagaaaat aatacactag atatgtataa tagtactaat
      421 atacaaacta aaagtaatgt aaaagtagat gattataaga atgagttttt acataaaagt
      481 agtaataacc ataaaattga tgattataag aatgtaacta aagaaaagag ttcaagcata
      541 aaccagaaaa attcagattc taataagaaa aaaagtatta aaaaaaataa atacctatat
      601 atagcaatag tattaggaat attattaata ggacaaggta tatatagttc ttataagatg
      661 aataatttaa ctgataaaat atctaaaaat aatgaaacta aaaatgaaga gtcaacagat
      721 tctagtttgg ctaagaaaga aaacaatata gaagaaaatg aagatatgaa taagacttct
      781 aatatcgata agagtaataa aaatattaat aaagaggata taaaatctaa aaaaaatact
      841 gtagagaatg aagatgataa agattctgac ttatttacag aggatgaatt gatgtctaag
      901 gcaaatactt taaaatcaga aaagaaaact aaatcttcta tacaactata taagaaagtt
      961 gcagatatag gtgaaaataa aggaaataca tctgaagcaa cttatcaagt tgctattctt
     1021 agtgaaaagt taaaagacta taaaactgct gaagaatatt ataaaatgta tgtggaaaat
     1081 tactcagaaa aagatgctta ttttgatgaa tcttattata acttaggtat gatgtactat
     1141 aacaatggtg atttaaaaag ttctaaattg acattaaaga agttggttaa taaggtgcca
     1201 aatagtatgt ataataattc aaaagtaaaa gaaattttaa aagaagaata gctaaattag
     1261 ataagaaaaa taaattattt tattttaaaa taatatgatt ataatatatt gacataccat
     1321 tttaaaaaat ttaaaatatt aattataaaa tgaggaggga taaaaaatgg agtttgaaaa
     1381 agggagtatt tcaattcata cagaaaatat ttttcctata attaaaaaat ggttatattc
     1441 tgataaagac atatttataa gagagcttat aagtaatgga tgtgatgcag taagtaaaca
     1501 caaaagactt gtatcattag gagaaatatc agagaataag tcatcagatt acaaaattat
     1561 agtatctgta aataaaggag aaggtacgct taaatttatt gacaatggaa ttggtatgac
     1621 tgaagaagaa ataaaaaaat acataaatca agttgctttt tcaggtgctg aagatttctt
     1681 taataaatat aaggataaga tggaagagtc aaatgacata ataggtcatt ttggattagg
     1741 attttattct gcatttatgg tgtctaaaaa ggtacaaata gatacacttt catatacaga
     1801 aggagcaact cctgtaagat ggatatctga aggtggtaca gaatacgaaa tatctgaatc
     1861 agatactaga aatgatagag gaactactat aacactattt attgatgatg atagtaaaga
     1921 gtttttagat gaatttactg taagagggat aataaataaa tactgttctt tcttaccagt
     1981 agaaatatat ttagaagatg ttgaaagatt agaaagagaa gctaaggaag cagaagaaaa
     2041 agctaaaaaa caaaaggaag atggaaaaga agaggttgta gatgcaaaag taattgagcc
     2101 tttaaacgac actaatccgc tatggttaaa atctccaaaa gattgtactg atgaggaata
     2161 taaagagttt tatagaaagg tattccatgt atttgatgag cctttatttt ggatacattt
     2221 aaatgtagat tatcctttca acttaaaggg tatactttac ttccctaaat taaaaaatga
     2281 gtttgagttg actgaaggaa aagtaaaatt atacaacaat caagtgtttg tggctgacaa
     2341 cataaaagaa gttataccag aatttttact tttattaaaa ggtgttatag actgcccaga
     2401 cttaccatta aatgtatcaa gaagtttctt acaaaatgac agagacgtaa gtaaaatttc
     2461 taaacatata attaaaaaag tagctgataa gttaaaatct ctttgtaaaa atgaaagaga
     2521 agaatataat aaattttggg atgatataca aatatttatt aaatacggtt gcttaaaaga
     2581 tgaaagtttc tatgagaagg taaaagaatg tatattattt aaaactattg atgatgagta
     2641 tataacatta caagattatc tagaaaaatg taaggacaaa cacgaaaata aggtatttta
     2701 tgtaagtgat aaggagcaac aatctcaata cattaaatta tttaaagaat acgacttaag
     2761 tgctgttgtt ttaaactcat caatagatac tcattttatt tcatttatgg aatataaaga
     2821 aaatggagtt aagtttaata gaatagatgc agacctttct gatgtattaa aagataaaaa
     2881 tgaaaataaa gatagtgaag aaaacaaaga agagatagct aagatagaag gtctgtttaa
     2941 agaaactgtc ggagaaagag taaagaatta ttctgtagaa ggattgaaaa atgaagacac
     3001 tccagctatg gtgttagtat cagaacaatc tattagaatg gcagaaatgc aatcaagatt
     3061 tgctggaatg gatttaggaa tgaattttga ggaagaaaaa actcttgtta taaatgagaa
     3121 cagtccaata atcaaaaaat tggtttctct taaagatgat gaggaaaaga aagataaggt
     3181 tacgcttatt tgtaatcaga tagcagattt agctttactt tctaataaag aattaaagcc
     3241 agacgagtta gattcatttg tccaaagaag taataaactt atgagtttgt tgatagaatt
     3301 ataaggttga atttgaatac attgaagtaa atttataaaa agtattcaat atcaatgata
     3361 ataaagaggt gtctcaaagt gaacttttta gttcatgagg cacttttttt atgtgaaatt
     3421 aaatgtattt taatcgtttc aataatgaaa aagaaattta tctctatttt tagacagact
     3481 attttttata tttgattaat ttttaaatat ttaagtaggt tctaataaaa aactctattt
     3541 agttaaatat agttaacttg atatataata aaataataca tataattaag taaaagattg
     3601 ttttttagtt tatgtttaaa tacataatta aattttgtta aaaaataaaa ttattaataa
     3661 aatagaaata ggaggtatta gcatgaattt taattataat ttgccagtaa atatcttatt
     3721 tgggagaggc agaattaatg atgtgggaaa agaagtatcc aaatatggca aaaaagtatt
     3781 gatagtaaca ggtaaaaata gtacaaaaaa aactggatta cttgataaaa caatagattt
     3841 actaaaagac tctaaaatag aatatgaagt ttttgataga gtagagcaaa atcctcttat
     3901 aactactata tattcaggag tagaaattat aaaagcaact ggatgtgatt gtgttttagg
     3961 gcttggtgga ggaagtatta tggatgctgc taagagtata gctttttcct ataaaaaccc
     4021 aggagattta aatgattata tatttggaat tagacaaggt gaagaagctt tacctataat
     4081 cttagttcca actacatcag gcacaggtag tgaaggaaat tgttttgcag tacttacaaa
     4141 ttcagaaact aaagataaaa aatctataaa gaagaatagt atgtatgcaa aatcatcaat
     4201 aattgaccca gaacttatgg ttacaatgcc aaagcatata attgcatcag ttggatttga
     4261 tgcacttgct cataatatgg aagcatatct ttctaatgga agaaacccta ttgcagatgt
     4321 acaagctatt tatgggattg aacttatatc agaaaaccta gtaaaggtat ataataatgt
     4381 taatgattta gaagcatggg aaaaaataac acttgcaagt actattggag gaatgacgat
     4441 tggtacagcg ggaacagcct tgtcacatgg tatggagcat cctgtaagtg gtcttagaga
     4501 tgtaatgcat ggaaaaggac ttgcagcttt gactcctgtt attatagaaa gttcatggga
     4561 tagtgatact gaaagatttg ggaatatttc taaaatatta ggaggaactt gtgcagaaga
     4621 ttgttcagat gcaattagaa attttcttaa aaaaatagat ttggatataa aactaagtga
     4681 tttaggtatt gaaaaatcag atatagattg gttaactgat aattgtatga aaatatcagt
     4741 tgcaaatatt aagagacatc caaagtactt taataaggaa gaaataaaag aaatgtaccg
     4801 taaatctcta taatctgatt ttacatattt aactttaaat aaaaattata aattaataat
     4861 aaaaaattta taattaatca atagcaaaaa aattgtgtat aggaattaga tacacaataa
     4921 acttgtctta agcttttatt ttactttcta ataaataaag gagtttaaaa aagggaagat
     4981 taattaagta atattttata aaagtcttcg atttaaaaac tttaagtaga ggtgaaataa
     5041 atgtttaatc ccaaaagaat aatattcgaa aaaggaactt tagacactga tgtgggtaaa
     5101 aatatataca ataaatttaa agataataaa gatgtagaga taattaatat atcatcaaat
     5161 agaattaaag aacatattcc aggtgaaaat ctatatgaac aatatagaag tgggaagcag
     5221 actatggtgg ttgggattag aaaaagtctg aagtttcaaa catgtaaacc ttctgctaat
     5281 taccaactac cacttgtcag tggatgtatg ggaagatgtg aatattgcta tttaaataca
     5341 caattaggtg ataaaccttt tataagggta tttgtaaatg tagatgaaat actagaacag
     5401 acaaaaaaat atattgaaga tagaaagcca caggtaacca tatttgaagg agcggctaca
     5461 tcagacccag ttccagtgga gccatatact aattccttga agaaaacaat agagtttttt
     5521 ggaaaagaag aaaaaggaag atttagattt gttactaaat acagtgatgt agatagctta
     5581 ttagatgcca aacataataa tcatacagaa atacgattta gtatcaatac tcctagaatt
     5641 ataaacgaac atgagcatta tacagcatct gcaaataaaa gaatagagtc agctgtaaaa
     5701 cttgctaggg ctggctatca gattggtttt ataatagcac ctgtgttttt atatgaaggt
     5761 tggaaggaag aatataaaaa tcttattaga agtataaaag aagctttacc aaaggatttt
     5821 gataaaaaaa ttatatttga agttatatct catagatata caactaaagc aaaaaataga
     5881 atattagaga tttatccaga tacactatta ccaatggaaa atgaagatag aaagtttaaa
     5941 tacggtcaat ttggatatgg taagtacgtg tacgataaag aacaattaga tgatatgaaa
     6001 gagttctttc ataaggaaat aaatggagta tttaaaaata gtttaataaa atatataata
     6061 tagcaaatct tatctaaaga tttgctatat tttattttta taataaagtg atgagtttta
     6121 gtactataat atgagaaaat taaaaagggc actatctatc aagaacattt tttctagttt
     6181 tgatactata aaggataaga gtaacaaaaa tatcaggagg agttataata tgcatgcatg
     6241 ggagtctatt caaattacgt tggatttaat agaagcaaac ttatcagaag aaatatcaat
     6301 taatgaatta gcaaataaag caaacttatc accattttac tatcagagac tatttaaaag
     6361 attggtaaat aagactgtaa tggagtatat aaagcttcgt cgtcttgcaa aagcttctga
     6421 atacttagca gaacataaga atcgtatact tgatgtagct ttaaaatttg gatttgctag
     6481 ccatgagaca tttacacgtt cctttaaaaa agcatatgga ttaactccag aaaaatatag
     6541 agcaaatcca gtaaaactca atcactttat taagccagaa ttaattttaa attatgctat
     6601 ggttgatgaa ggtgttccac tgattgcaga tgatatagtg attgaagtca ctcgtaagac
     6661 attgagtaat ccaaggacgt ttgttggaat agaaatggaa gtacctattt gccagttggt
     6721 gggcggtgaa acaactggta ttgcaatagt agaggaatta tggatgaaac ttggaagtca
     6781 aagacataat ataccaaatc aaattccagg tggaaatgaa tttgctgctc tttatatggg
     6841 aaatgcaaaa gaaggaaatt gtatgtatat ggcaggtgta gaggtagaat tagggacatc
     6901 agtagaagga tatagtactt ttgagttacc agctaaagaa tatttagtat gtggatttga
     6961 ggcagaaaac tttaatgagt tagttaattc agctgttttt aaagcggata aatttatgga
     7021 aagatggatg aagaagcata atttaacaac tgctgatttt gcaattgaga tgtattaccc
     7081 cactacacct gaagtagcat atctggagca ttggattgtt ccaattccta ttgaacaata
     7141 gaaaggatga tgaatatgaa ttggcaagat acgttttttc aagatactaa acctacattt
     7201 gagcaaatta atgaatttat taataatcca ttatgggaca agttaaataa aactttggtt
     7261 tctacttata atgtagaacc taagcttgaa tacagtaaat gttcaatgca aagaggctgg
     7321 aatgtaaaat acaaaaaaag aggtaaatca ctatgtactt tatatccaca agaaggttat
     7381 tttaaagctt tggttattgt aaatgaaagc aatagagtag aggttgattt atttattaat
     7441 acttgttgtg attatatcaa acaaatatat aatgaagtaa aattttttaa tggtggtaag
     7501 tggttaatga ttcaagtaga tagtttgttg gtattaaacg atatgcttga actaattaaa
     7561 tttagaatat aaaatataaa acaggatgaa tatataagtc atcctgtttt tatattcaat
     7621 cagataaatt aatatataga ctatagtata aataatttag caaatttaaa tttactttgt
     7681 ttttctacat tccttgcaat taagtccaca tttatttttt tcttgcatat tggtagcacc
     7741 ccaattagca aattgttgta aaataggtac aatacttttg cctttacttg ttagtgtata
     7801 ttcaaccttt ggaggaattt cattatattg tttacgatga acaataccat cagactctaa
     7861 atcttttagt gattgagcta gcatagtgtt tgtaattcca tctaattttc tttttatgct
     7921 actatatcta atgggatttt caactgctaa catacatata ataggtaatt tccacttacc
     7981 accaacaata tctaatgcat aacgaattgg acaggttaat tcttcaacat ttttagttat
     8041 atttttttca ctatttatac ccattttaat tactccttag ttataatagt atctttttta
     8101 tactttataa gaaaaaactg catacttgtt ttttctgtat tttatattaa aattatatca
     8161 aaggataatc cttaatacaa gtatttaaaa ggggtgtaag aattatgaaa gcattagaat
     8221 ttttgaacaa ggcaggagtt tattatttag caacagctga tgagaacaat caagcacatg
     8281 tgcgtccttt aggatttgtt atggaataca atggtaaact tacattttgt acatctaatc
     8341 aaaaggatat gtttaagcaa cttattttta atccaaaggt tgagctttgt tgtatagacc
     8401 aaaaccttaa tactctacgt attttaggtg aagcagtatt tgttacatca aaagaaacac
     8461 aagctaaagc acttgaaata atgccaagtt taggtcagat gtactctgta ggagatggta
     8521 agtttgaaat attctctatt gataaggcaa aagttagttg ttgttcaatg agtggtaaaa
     8581 agattgatgt agaactttaa ttataattga atcgatacac atagtgaaaa ttgaaaaaac
     8641 gttatcactg aaatattgaa gatttaatta aaatgcaata aaatgttttt aaataatgaa
     8701 tggataaaaa tttttagtta ttagatttgt atttcaataa tgatatgtta ttttgaataa
     8761 ttacatacta gaaataaggg caatcaaaat ttattttata attggctaga cttcttttaa
     8821 aaaatatttt aactataatt gatttttatt taatcgaaaa aaatattatg actataaaaa
//
LOCUS       DAFNNB010000152         8638 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0157, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000152 DAFNNB010000000
VERSION     DAFNNB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0157"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     regulatory      118..228
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            332..703
                     /locus_tag="L3M60_003007"
     CDS             332..703
                     /locus_tag="L3M60_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrdX family protein"
                     /protein_id="HBN6227641.1"
                     /translation="MIIITNNPKVKEEVQDREVLFKDTTYIGILEASRDLIHEGYELL
                     SHPLYGSVKPNETPYRTVVLKKGNRLDINSLTLIEEAIITASKFQNNKKTPKWTESVQ
                     DDFRVIDYDIFYNTIQRMQYE"
     gene            799..1746
                     /gene="trxB"
                     /locus_tag="L3M60_003008"
     CDS             799..1746
                     /gene="trxB"
                     /locus_tag="L3M60_003008"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBN6227642.1"
                     /translation="MENVYDLVIIGSGPAGLAAGLYGARAKLKTLILEKDKTGGQIVI
                     THEIANYPGSVPNATGPSLIARMVEQCKEFGAEMLRDNIVDTELDGDIKVLKGEKAEY
                     RAKAVIIGTGATPRKIGCPGEKELTGKGVSYCATCDADFFEDFEVFVVGGGDSALEEA
                     MYLTKFARKVTIVHRRQGFRCAKSVEEKAKANPKIEFLLDTVIEEIKGDGILESVVFK
                     NKVTGETHEYFADEEDGTMGVFVFVGLDAQTDLFKGKVDMDEKGYIITDEDMRTNIPG
                     VFAAGDCRSKTLRQVVTATNDGAIASIVAEKYIDEKFGN"
     gene            1832..2149
                     /locus_tag="L3M60_003009"
     CDS             1832..2149
                     /locus_tag="L3M60_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductase thioredoxin"
                     /protein_id="HBN6227643.1"
                     /translation="MLDLDKATFEEEVLNAEGFVFVDFWSEGCEPCKALMPDVHKLAE
                     TYGDKIKFCKMDTTKARRLAIKQKVLGLPTMAIYKDGEKVDEVTKDDATVPNIENMIK
                     KYL"
     gene            2416..3702
                     /locus_tag="L3M60_003010"
     CDS             2416..3702
                     /locus_tag="L3M60_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006439082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase component B
                     subunit"
                     /protein_id="HBN6227644.1"
                     /translation="MRLELGKIFIKDIQFGDVTEVKDGVLFINKQEMLQEIGGDEHIK
                     SIDIELARPGESVRITPVKDVIEPRVKVEGNGGIFPGIMSKVDTVGEGKTHALKGVAV
                     VTTGKIVGFQEGIIDMTGEGAKYTPFSKLNNVVVIAEPIDGLKQYAHEKAVRMIGFKA
                     AMYLGEVARNLTPDEVSVYETKPLLESIKEYPELPKVGYVYMLQTQGLLHDTYVYGVD
                     AKQIVPTLLYPTELMDGAIVSGNCVSACDKNPSYVHINNGVVEDLYARHGKDINFVGV
                     IITNENVYLADKERSSNWTSKLCKYLGLDAVIVSQEGFGNPDTDLIMNCKKIEMQGVK
                     TVIVTDEYAGRDGGSQSLADADVRADAVVTGGNANQVVVLPKLDKVIGHLEVVDVIAG
                     GSDGSLRADGTIEVEIQAITGATNETGFGHLTAKGY"
     gene            3815..4288
                     /locus_tag="L3M60_003011"
     CDS             3815..4288
                     /locus_tag="L3M60_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011986236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:3947..3949,aa:Sec)
                     /transl_table=11
                     /product="glycine/sarcosine/betaine reductase complex
                     selenoprotein A"
                     /protein_id="HBN6227645.1"
                     /translation="MSLLSNKKVLIIGDRDGIPGPAIEECVKTVEGAEVVFSSTECFV
                     UTAAGAMDLENQNRVKDAADKFGAENVVILLGAAEAEAAGLAAETVTAGDPTFAGPLA
                     GVALGLSVYHVVEEPIKSLFDESVYEDQISMMEMVLEVEEIEEEMSGIREEFCKF"
     gene            4320..5630
                     /gene="grdB"
                     /locus_tag="L3M60_003012"
     CDS             4320..5630
                     /gene="grdB"
                     /locus_tag="L3M60_003012"
                     /EC_number="1.21.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5367..5369,aa:Sec)
                     /transl_table=11
                     /product="glycine reductase complex selenoprotein B"
                     /protein_id="HBN6227646.1"
                     /translation="MGKLKAVHYINQFFAGIGGEEKADTKPHVAETLPPISLQLDKLL
                     GEDIEIVGTVVCGDSYFNENIDSASEEVLSMVKGFEPQLFIAGPAFNAGRYGVAAGTI
                     TKVVKDALNIPALTGMYVENPGADMFKKDVYVVETSDSAAGMRKALPKIAKLAVKLAN
                     GEEIGTPKDEGYIARGIRVNYFHEDRGSKRAVDMLVKKIKGEPFETEYPMPNFDRVDP
                     SKAVKDLSKCKIALVTSGGIVPKGNPDRIESSSASKYGTYSIAGVMDLTEETYETAHG
                     GYDPVYANLDADRVLPVDVLRDLEKEGVIGKLHETFYTTVGNGTSVANSKKYASEIGA
                     ALVADGVDAVILTSTUGTCTRCGATMVKEIEKTGLPVVHMCTVVPISLTVGANRIVPT
                     IAIPHPLGNPALDPTEEKALRRGLVEKALNALTTEVDGQTVFEK"
     gene            5877..7409
                     /locus_tag="L3M60_003013"
     CDS             5877..7409
                     /locus_tag="L3M60_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014520415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III family protein"
                     /protein_id="HBN6227647.1"
                     /translation="MTYPVLKGAGYVLIHTPDMIVQNGSTCTVERATNPDSEFLKEVS
                     NHIRSYEDVVNYMPNQVYIGNRRPEELRDLPMPWCEQKIEGTRNGKFGEIMPQDEFIA
                     LMQISDAFDLVKLSQEFIDEVKPKIENNYPEIAPFVGKLKGDDIEEGKELVATHIAEG
                     LYHDGKFVGYVKRAHDVDVNLNAHTMFENLVVKASGVLSAIQMLRHSKIDPAEIDYVI
                     ECSEEACGDINQRGGGNFAKSIAEIVGLQNATGSDTRGFCAAPTHALIQAAALVKAGI
                     HKNVMVVAGGASAKLGMNAKDHVKKGLPVLEDVVGGFAVLVSENDGVNPVIRTDLTGK
                     HTVGTGSSPQAVMTALITSGLDRANLKITDVDVYSVEMQNPDITKPAGAGDVPEANYK
                     MIGALAVKRGDLEKKELKDFVSNKGLPGWAPTQGHIPSGAPYIGFLIDDLTTGNRNRA
                     MIVGKGSLFLGRMTNLFDGVSFIAERNTGVTEETSGISKDEIKKIIAESMKKLALDML
                     EE"
     gene            7420..8565
                     /locus_tag="L3M60_003014"
     CDS             7420..8565
                     /locus_tag="L3M60_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine reductase"
                     /protein_id="HBN6227648.1"
                     /translation="MSKKVIADVFLEVANAIESGEFGKKVKIGVTTLGSEHGVENMVN
                     GAQLAKSNLFDIVLIGPKVETDLEVVEVNDEKEMHAKMEELLDSGYIDACVTMHYNFP
                     IGVSTVGRVITPAKGKEMILATTTGTSATNRIEAMVRNAIYGIATAKSMGNKCPKVGI
                     LNVDGARQVEKCLKELKDNGYDMEFADSIRADGGCVMRGNDLLVGAPDVMVTDTLSGN
                     IFMKVFSSYTTGGDYEAQGFGYGPGVGEDYDRKVLIVSRASGSPVVANALKYAYDVVK
                     GDISNVARNEFAKVKKAKFADIISSLTKKEVKAEKVEVKMPDKEIVTRQIAGVDIMDL
                     EDAVSELWKNGIYAESGMGCTGPIVLVNEAKGDLAVETLVKAGYTAK"
BASE COUNT         3383 a         1004 c         1777 g         2474 t
ORIGIN      
        1 tgcaaatttt atagaatgtt tatatactta agtcaacaga gatagaaaaa agctatttat
       61 agaaaacaaa ggataaattg cttaatttta aattttctga aaaaaataat aatccggatg
      121 aagagtatgg gagactgctt tataaaaagc gaccgaaggg acaagtttaa tcttacttac
      181 ccaaaagatt agatgaaatt ctcaggtaaa ggtgactgta cttggacggg gctctgaaaa
      241 tgattttaac agggaaggca aattgggaga tttgtttttc ctgtttttta tttatcaaga
      301 aataaataaa agtttgaaag agagtaaaaa aatgataata attacgaata atccaaaagt
      361 aaaagaagaa gttcaagaca gagaagtttt gtttaaagac acaacttata ttggaatctt
      421 agaagcaagt agagacttga tacatgaagg ttatgaactt ttatctcatc cactctatgg
      481 gagtgtaaaa ccaaatgaaa caccttacag gactgttgta ctaaagaaag gaaatcgttt
      541 ggatataaac tctttaacct taattgaaga agcaatcatc actgcaagta aatttcagaa
      601 taataaaaag actccaaaat ggacagaaag tgttcaagat gattttagag taattgatta
      661 tgatatattt tataatacaa tacaaagaat gcagtatgaa taaagaaaat tgttaaaaag
      721 ttattaaaac agcctaaata ttaaatctaa tttaatatta aataaaatga gaaatttaat
      781 tggggaggat aagtcaaaat ggaaaatgtg tatgacttag taataattgg ttcaggacca
      841 gcagggcttg cagcaggtct ttatggagca agagcaaagt taaaaactct aatcctagaa
      901 aaagacaaga ctggtggaca aattgtaata acacatgaaa ttgcaaatta tccagggtca
      961 gttccaaatg caacaggacc aagtttaata gctagaatgg ttgaacagtg taaagagttt
     1021 ggagcagaaa tgctaagaga caacattgtt gatactgagc tagatggaga tataaaagtt
     1081 ttaaaagggg aaaaggcaga atatagagca aaagcagtaa taattggtac aggagcaaca
     1141 ccaagaaaga ttggttgtcc tggagaaaaa gaacttactg gtaaaggggt ttcatattgt
     1201 gcaacttgtg atgcagattt ctttgaagat tttgaagtat ttgttgtagg tggaggagat
     1261 agtgcattag aagaagcaat gtatttaact aaatttgcta gaaaagtaac aattgtacat
     1321 agaagacaag gatttagatg tgctaagagt gttgaagaaa aagcaaaagc taatccaaag
     1381 atagagtttt tattagatac agtaattgaa gaaatcaaag gtgatggaat attagaatca
     1441 gttgtattta aaaataaagt tactggagaa acacatgaat actttgcaga tgaagaagat
     1501 ggaacaatgg gagtatttgt tttcgttgga ttagatgctc aaactgattt attcaaaggc
     1561 aaagttgata tggatgaaaa aggttacata ataactgatg aagatatgag aactaatata
     1621 ccaggggtat ttgcagcagg agattgtaga tctaagactc taagacaggt tgtaactgca
     1681 actaatgatg gggcaattgc atctatagta gcagagaaat atattgatga aaaatttgga
     1741 aactaatatc aaatttggaa actaatatct gtaaattaaa tttaatgaac aatattaaaa
     1801 taaaataaaa atttcaagga ggaataagag aatgttagat ttagataagg ctacatttga
     1861 agaagaagtt ttaaatgcag aaggttttgt atttgttgat ttttggagtg aaggttgtga
     1921 gccttgtaaa gctttaatgc cagatgttca taaattagct gagacttatg gagataaaat
     1981 aaaattctgt aagatggata caacaaaagc tagaagatta gctataaaac aaaaagtatt
     2041 aggtcttcca actatggcta tatacaaaga tggagaaaaa gtagacgaag ttactaagga
     2101 tgatgctaca gttcctaata tagaaaacat gattaaaaaa tatctttaat atgttgataa
     2161 agtattaaca gtaaaagaaa aatctatttt tctctgaaga taagaagaaa aaagttttta
     2221 tgtctaactt tgttaaaaaa ataattttaa ataaaataat ttttttacaa taaacatttt
     2281 ttagtaataa aataaagatt taagacataa aaacacaaga atatatctta aaaaaataag
     2341 ataaattgta aaatgatata attttgagaa aaactcaaat gcatatacac aaattaaata
     2401 acaggaggtg caagtatgcg tcttgaatta gggaaaatct ttataaaaga tattcaattt
     2461 ggtgatgtaa cagaagtaaa agatggagtt ttattcatca ataagcaaga gatgctacaa
     2521 gaaattggtg gagatgagca tattaaatct atagatatag aattagctcg tccaggtgaa
     2581 agcgtaagaa taacacctgt aaaagatgtt attgagccaa gagtaaaagt tgaaggtaat
     2641 gggggaatct tccctggtat catgtctaag gttgacactg tgggagaagg aaaaactcat
     2701 gctttaaaag gtgtagctgt tgtaactact ggaaaaatag taggtttcca agaaggtata
     2761 atagacatga ctggagaagg agctaagtac actccatttt ctaaattaaa taatgtagta
     2821 gtaatagctg aacctattga cggtttaaaa caatacgctc atgaaaaagc tgttagaatg
     2881 ataggattca aagcagcaat gtacttagga gaagtagcaa gaaatcttac tccagacgaa
     2941 gtttcagtat atgaaactaa acctcttctt gaatcaataa aagaatatcc agagcttcca
     3001 aaagtaggat atgtttacat gcttcaaact caaggtttat tacatgatac ttatgtatat
     3061 ggagtagatg caaaacaaat agtaccaact ttattatatc caacagaatt aatggacgga
     3121 gcaattgtaa gtggtaactg tgtttctgca tgtgacaaaa acccaagtta tgttcatata
     3181 aacaatgggg ttgttgaaga tttatatgca agacatggta aagatataaa ctttgtaggt
     3241 gtaattataa ctaatgaaaa tgtttaccta gcagataaag aaagatcttc aaactggact
     3301 tctaaattat gtaaatattt aggattagat gcagttatag tttcacaaga aggttttgga
     3361 aatcctgata ctgaccttat aatgaactgt aagaagatag aaatgcaagg tgttaaaaca
     3421 gttatagtta cagatgaata tgctggacgt gatggaggtt ctcaatcact tgcagatgca
     3481 gatgtaagag ctgatgctgt tgtaacaggt ggaaatgcca accaagttgt agttcttcct
     3541 aagttagata aagttattgg tcatttagaa gtagtagatg taatagctgg tggttctgat
     3601 ggaagtttaa gagctgatgg aactattgaa gttgaaatcc aagctataac aggtgcaact
     3661 aatgaaacag gatttggtca tttaactgct aaaggatatt aagaagtatt tcattaaacg
     3721 attgagtaat gttaataaaa catattatag ataaattata ataagtgtta tagatatata
     3781 aaaatatatt agaaatataa taaggaggat tattatgagt ttacttagta ataaaaaggt
     3841 tcttataata ggtgaccgtg atggtatacc aggacctgcg atagaagaat gtgtaaaaac
     3901 agtagaagga gcagaggttg ttttctcatc tacagaatgc tttgtctgaa cagctgctgg
     3961 ggctatggac ttagaaaatc aaaacagagt taaagatgct gctgataaat tcggagctga
     4021 aaatgttgtg attttactag gtgctgctga agccgaagct gcaggtcttg cagccgaaac
     4081 agtaactgct ggagatccaa ctttcgctgg accacttgct ggagttgcct taggattaag
     4141 tgtttaccac gttgttgagg agccaattaa atcattattt gatgaaagtg tatatgaaga
     4201 ccaaataagt atgatggaaa tggttttaga agttgaagaa atagaagaag aaatgtctgg
     4261 tataagagaa gaattttgta aattttaatg cctaaaagca aaaggggtga aactaagaga
     4321 tgggaaaact aaaagcagta cattatatta atcagttctt tgctggtata ggtggagaag
     4381 aaaaagctga tactaagcca catgtggcag aaactttacc tccaataagt ttacaacttg
     4441 ataaattatt aggtgaagat attgaaatag ttggaacagt agtttgtgga gatagctatt
     4501 tcaatgaaaa tattgatagt gcaagtgaag aagttttatc aatggtaaaa ggttttgaac
     4561 cacaactttt catagctggt cctgcattta atgctggtag atatggagtt gctgctggaa
     4621 caataactaa agttgtaaaa gatgctttaa atataccagc tttaacaggt atgtatgttg
     4681 aaaatccagg agcagatatg ttcaaaaagg atgtttatgt agtagaaact tctgattctg
     4741 cagcaggtat gagaaaagca cttcctaaga tagctaaact tgcagtaaaa cttgctaatg
     4801 gagaagaaat tggaactcct aaagatgaag gatacatagc tagaggaata agagttaact
     4861 acttccatga agacagaggt tctaagagag ctgttgacat gttagttaag aaaataaaag
     4921 gtgaaccatt tgaaactgaa tacccaatgc caaactttga tagagttgac ccaagcaaag
     4981 ctgtaaaaga tttatcaaaa tgcaaaatag ctttagttac ttctggtggt atagttccaa
     5041 aagggaatcc agatagaata gagtcttctt cagcttctaa atatggaact tattctatag
     5101 caggagttat ggatttaaca gaagagactt atgaaactgc acatggtgga tatgacccag
     5161 tgtatgcaaa tttagatgca gatagagtat tacctgttga tgttcttaga gacttagaaa
     5221 aagaaggtgt aattggaaaa ttacatgaga ctttctatac aactgtaggt aatggtacat
     5281 ctgttgcaaa ctctaaaaaa tatgcttctg aaattggagc agcattagtg gctgatggtg
     5341 tagatgcagt aatattaact tctacatgag gtacttgtac tcgttgcggt gcaacgatgg
     5401 taaaagaaat agaaaaaact ggacttccag tagttcatat gtgtacagta gtacctattt
     5461 cattaacagt aggggctaat agaatagttc caactatagc aatacctcat ccacttggaa
     5521 atccagcgct tgaccctaca gaagaaaaag ccctaagaag aggacttgta gaaaaagcat
     5581 taaatgcttt aactacagaa gtagatggtc aaacagtatt tgaaaaataa aaataacaca
     5641 cacatatatt atatagataa aaatataaac acacactaaa aataaaaaat aactatacac
     5701 aaaaaaataa tatagatttc atgaatggat aaattggagt tattcattca tgaaatctta
     5761 aaaaatgcca aaatacataa acaataatag aattaaatat acaataaaat acaataacta
     5821 gaatttttaa taagattaaa aaataagtgt tcataaaatt tatggaggtg cacaaaatga
     5881 cttatccagt tttaaaaggg gcaggctatg tacttattca tactccagat atgatagtgc
     5941 aaaatggaag tacttgtaca gttgaaagag caacaaatcc agactcagaa tttttaaaag
     6001 aagtaagtaa tcatataaga agttatgaag atgtagttaa ctacatgcca aatcaagttt
     6061 atataggaaa tagaagacca gaagaattaa gagatttacc aatgccatgg tgtgaacaaa
     6121 agattgaggg aactagaaat ggtaagtttg gagaaataat gcctcaagat gagttcatag
     6181 ctttaatgca aataagtgat gcatttgatt tagtaaaatt atcacaagaa tttattgatg
     6241 aagtaaaacc aaaaattgaa aacaactacc ctgaaattgc accatttgta ggtaaattaa
     6301 agggtgatga tatagaagaa ggtaaagaat tagtagcaac acacatagct gaaggattat
     6361 accatgatgg aaaattcgtt ggttatgtaa aaagagcaca tgatgtagat gttaacttaa
     6421 atgcccatac aatgtttgaa aatctagtag taaaagcatc tggagtttta tctgctattc
     6481 aaatgctaag acatagtaaa atagaccctg ctgaaataga ttatgttatt gagtgttcag
     6541 aagaagcttg tggagatata aaccaaagag gtggaggaaa ctttgctaaa tcaatagctg
     6601 agatagtagg acttcaaaac gcaactggtt ctgacacaag aggattttgt gcagcaccaa
     6661 ctcatgcatt aattcaagca gcagctttag taaaagctgg tatacataaa aatgtaatgg
     6721 tagtagcagg tggagcaagt gctaagcttg gaatgaatgc taaagaccat gttaaaaaag
     6781 gtcttcctgt acttgaagat gttgttggag gatttgcagt acttgtatct gaaaatgatg
     6841 gtgtaaatcc agttataaga actgatttaa caggtaagca tactgttgga actggttctt
     6901 caccacaagc agttatgaca gcacttataa cttcaggtct tgatagagct aatttaaaaa
     6961 taacagatgt tgatgtatat tcagttgaaa tgcaaaatcc agatataact aagccagcag
     7021 gagcaggaga tgttccagaa gcaaactaca aaatgatagg agcacttgct gttaaacgtg
     7081 gagacttaga aaagaaagaa ttgaaagatt ttgtttctaa caaaggatta ccaggatggg
     7141 caccaactca aggacatata ccatcaggag ctccttatat aggattttta atagatgatt
     7201 taactacagg aaatagaaat agagctatga tagttggaaa aggtagttta ttcttaggaa
     7261 gaatgactaa cttatttgat ggagtatctt ttatagctga aagaaataca ggtgttactg
     7321 aagagacttc aggaatatct aaggatgaaa ttaagaagat aatagctgaa tctatgaaaa
     7381 aactagcatt agatatgtta gaagaatagg ggtgaggata tgtcaaaaaa agtaatcgca
     7441 gatgtattct tagaagtagc taatgcaata gaaagcggcg aatttggcaa aaaagtaaaa
     7501 attggtgtta ctacacttgg aagtgaacat ggtgtagaga acatggtcaa tggtgcacaa
     7561 ttagcaaaat ctaatttatt tgatattgtg cttattggac caaaagtgga aactgacctt
     7621 gaagtagttg aagttaatga tgaaaaagaa atgcatgcaa aaatggaaga attattagat
     7681 tcaggatata ttgatgcttg tgttactatg cattataatt ttccaatagg tgtatctaca
     7741 gtaggtagag taataactcc agcaaaagga aaagaaatga tacttgcaac aacaacagga
     7801 acaagtgcta ctaatagaat agaggcaatg gtgagaaatg ccatttatgg tattgctaca
     7861 gcgaaatcaa tgggtaacaa atgcccaaaa gttggaatac ttaatgttga tggagcaaga
     7921 caagttgaga agtgcttaaa agaattaaaa gataatgggt atgatatgga atttgcggat
     7981 tcaattagag cagatggtgg atgtgtaatg agaggtaatg atttacttgt aggtgcacct
     8041 gatgttatgg ttactgatac attatctgga aatatattta tgaaagtatt ttcttcatat
     8101 actacaggtg gagattatga agctcaagga tttggatatg gacctggagt tggagaggac
     8161 tatgatagaa aggtacttat agtatcaaga gcatcaggtt ctccggtagt agcaaatgct
     8221 ttaaaatacg cttatgatgt cgtaaaggga gatataagta atgttgcaag aaatgaattt
     8281 gcaaaggtta aaaaagcaaa atttgctgac attatatcct ctcttactaa aaaagaagtt
     8341 aaagctgaaa aagtagaagt taaaatgcca gataaagaga ttgttactcg tcaaatagct
     8401 ggtgttgata taatggattt agaagatgca gtgtctgagt tatggaaaaa cggaatctat
     8461 gctgaaagtg gtatgggttg tacaggtcca atagttttag taaatgaagc taaaggagac
     8521 ttagcagttg aaactttagt taaagctgga tatactgcaa aataagtaca ctttttatgt
     8581 aaataagaac atatacttat aatcaataaa attataagta tatgttcttt ttattttc
//
LOCUS       DAFNNB010000153         8513 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0158, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000153 DAFNNB010000000
VERSION     DAFNNB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0158"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            170..1402
                     /locus_tag="L3M60_003015"
     CDS             170..1402
                     /locus_tag="L3M60_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HBN6227649.1"
                     /translation="MQEREELVLNPVPKEERVGWLAPLFNMLGSNIAISELMVGGTLI
                     LGMTLSNMIITSIIGNLILVAIIMIQGYIGYKEGLNTYVLAKGAFGEIGGKYLISLLL
                     GITSFGWFGVQAGVAGLSVQKIFPSVNLTLVTVILGLFMVVFALYGFKAMAKFNYLVI
                     PPLMILMVWGVFKAFSTYGVEAIYNYTPQTTMSMVEGLNIVVGLVIVGAIISPDQLRY
                     TRRVKDIWIISFLGLGIISLFQQVAAGVMSMGAPTWDITEVLANLGFGWVAFVILILA
                     SWSTNVSNAYSGGLALKTIFPNVKRNVLTLVAGLIGTIIAATGIIFKFQSFLSFLGIA
                     VPAIAGIMWCEYYFIQGRTYKHREGINWIAVISWLIGFAASYYSSKINFLIPPINGIV
                     VSMVIYYVLMKCFGIKDK"
     gene            1416..2222
                     /locus_tag="L3M60_003016"
     CDS             1416..2222
                     /locus_tag="L3M60_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HBN6227650.1"
                     /translation="MGRKVKIGIIQQHSVLGDVKKNIEKAVEMIDDLGKQGADIICLP
                     ELFATEYNLESLGGVKTLELIREHNKYIEKSMSEAAKRNNVYLISPYGTLEKGSTHVY
                     NSAVIFNRKGKIMGEYCKNHLWSLEAVYFKVGENVEVYEADFGRFGVMICYDAGFPEV
                     SRELTLKGSEIIFIPSAWRIQDEDMWDLNVSQRALENTVYTVGVNLVSNDSNLILFGK
                     SKICNPRGTVITQLDTYREKYVLSEIDLDEIKKYREDIPYLKDRKSNYNI"
     gene            complement(2334..3203)
                     /locus_tag="L3M60_003017"
     CDS             complement(2334..3203)
                     /locus_tag="L3M60_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6227651.1"
                     /translation="MDLLHLKYFQTVARMEHITKASHKLNIAQPALSKTISSLEKELG
                     VQLFDRKGRYIVLNEYGRLFLKRVDSILDLVESSKKELQDTSLENTGEVKILSPAAAN
                     VLPPLLSNFRKLYPNITFNVSHTLPSSYKKSDFDLYISSSFTKLNSENSITLTCEEIL
                     LGVSINHPLSLKDEVYLSEVSDENFIVITKGENYREVIDILCESANFKPKIAFESDSP
                     YTIYALIKSLQGVGFICGKSWGLSQDPEIKLLHIKDIEFKRYLNLSWFSENYESKAVL
                     LFKNFLINYFKNI"
     gene            3575..5002
                     /locus_tag="L3M60_003018"
     CDS             3575..5002
                     /locus_tag="L3M60_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp14"
                     /protein_id="HBN6227652.1"
                     /translation="MKLTKSIPSMLCLSIVLSLSPVSYANEISRTTVENTLVGQDRYQ
                     TAVEVSKKGWSSANEAVVVNSESIVDALAVTPFAKLKNAPILLTEKNNLNSQTEKELK
                     RLGVKKVYIIGLSGSVSNDVQKQLESNNLTTDRVGGSDRYQTALSIAKKIEELKDISE
                     IAIVNGYTGLADAVSIASVAATNGMVILPVSDASGISSFKNFIESKNISKSYIIGSTN
                     AVSDKIKQSLPNSERIGGADRNETNGKVIEKFYTSNKLNNVFVAKDGMKKQNQLIDAL
                     AVGVLASKENSPVLIVGEQLSATQKSVFSNKNTTAITQVGSGGNENAFKELKDLQVNS
                     NNKRVMYVTNTDKVNIRSDATIEASVIGALNNGDEVEVLEVLKTGWVKIKYNEGIGYV
                     SGSYLTNNKPDNSNENIKIKYVKEKDGLNVRKGPSTEDEKIGHLSYGSKVETIEMFAT
                     GWVKIKYNGGYGYVSNDYLSDTPVI"
     gene            complement(5081..5350)
                     /locus_tag="L3M60_003019"
     CDS             complement(5081..5350)
                     /locus_tag="L3M60_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227653.1"
                     /translation="MRFEYYYLIQDITGILLAFIGFRMSIIGFRILSIKGISINTLLL
                     VIKYCLFTIAGLNLLISKFGIRHWIWSVCMFLISIIINPRIKVSK"
     gene            complement(5353..6795)
                     /gene="nhaC"
                     /locus_tag="L3M60_003020"
     CDS             complement(5353..6795)
                     /gene="nhaC"
                     /locus_tag="L3M60_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HBN6227654.1"
                     /translation="MKMNTERKIPSFRYSLLVMLAIIALTSVGIVVFNASITTMFLLS
                     WLIVVPAAMKLGYTNDEIEAFGFEVGKDAFQSNLIILSVGVLIAAWIAAGTIPTVVYS
                     GLTIITPKYFLLTTLIVCSLTSVATGTSWGTLGTSGIAMMSIGTSMGIPLGLTAGAVI
                     SGAFFGDKMSPLSDSTNLAAAVCKTDVITHMKHMFYTTGPAYVICVVLYTVIGFKYSN
                     NTIDYVQINQIKDVLNSNFHIGLVAMIPIIFLLLLLLLQKPPIISILSSAIMGLIIAV
                     FQEGEKIGDVLNYMLSGFTIDTGFVYADKLLNRGGIMSMAETVLLVFVVFVIAGILQK
                     TGFLEVLLQPLINKIGKSRTKLVGSTFIVSYFANAFSSSMMFTSVFVGTLMSPIYKEF
                     KLKPQNLSRIIEDTATLGGPLIPWNSNAVFCAQTLGVSPLKFIPYCFLSWITPIISFI
                     YGVTGFTMLTYTDNEIEELEIFDTIQKRVD"
     gene            complement(6820..7986)
                     /locus_tag="L3M60_003021"
     CDS             complement(6820..7986)
                     /locus_tag="L3M60_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBN6227655.1"
                     /translation="MNYNFNEIVDRSNNFSSKWSEMEKKYGTNDLLPMWVADMDFKAA
                     PCIIDSLKNRIEQEIYGYTTRPDSYNESIVSWLDRRHNWKIKSEWLIYSPGVIPAISL
                     LINELTKENDKIMIQEPVYSPFNSVVKNNSRELVISPLQKLEDGNYMMDYEDIENKIK
                     DVKLFILCNPHNPVGRVWTKDELKKLGDICLKHNVKIISDEIHSDIILKKHKHIPIAS
                     ISKEFEKNTITCMAPTKTFNIAGLQSSYVILPDEKDYKLLDDAFTRIDIKRNNCFSLV
                     ATEVSYNNGEPWLESFLEYLESNIDFAIKYINENMPKLKVRKPEGTYLLWVDFSALGL
                     SDKELESKLVQKGKVALNQGNSFGIGGSGYQRINLACPKGMLEEALRRIKNAIN"
     gene            complement(8211..>8513)
                     /locus_tag="L3M60_003022"
     CDS             complement(8211..>8513)
                     /locus_tag="L3M60_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6227656.1"
                     /translation="KDKNLMKYDYNFKRSLDEYIFILDNLKKAYDLKERIGRKSLYKI
                     ALEENRFLTEQQIRNILLELQEFGLVNILVGRGGSIITSKGVEFLKNINRSNKLNS"
BASE COUNT         3102 a         1117 c         1326 g         2968 t
ORIGIN      
        1 gactatttac atatttataa cttaaattat atcaaattga taacaaacta gatattatac
       61 ttttttgtag aatagaatta caattatata aagaaaaatt ctatttatag tgaaaaaaaa
      121 gtagaaaaaa gtagaaatta ttcttttata aagaaaggga gggataatat tgcaagagag
      181 agaagaatta gttttaaatc cagttccaaa agaggaaaga gttggatggt tagctccatt
      241 atttaatatg cttggttcta acatagctat atctgaacta atggttgggg gaactttaat
      301 tttaggaatg actctttcta atatgattat tacatcaatt ataggaaact taatattagt
      361 tgcaattatt atgatacaag gctatatagg ttacaaagaa gggctaaata cgtatgtatt
      421 agctaagggg gcatttggag agataggagg aaagtatctt atatctctat tacttggtat
      481 aactagtttt ggatggtttg gtgtacaagc aggggttgca ggattatcag tacaaaagat
      541 attcccgagt gtaaacctaa cattagttac agttatactt ggattattta tggttgtttt
      601 tgcactgtat ggctttaaag ctatggctaa attcaattac ttagttatac cgccacttat
      661 gatattaatg gtctggggag tttttaaagc tttttctact tatggagttg aagctattta
      721 taattatact cctcaaacaa ctatgagtat ggttgaaggt cttaatattg tagtaggtct
      781 tgttatagtt ggtgcaatta tatcacctga ccagttaaga tatacgagaa gagtaaaaga
      841 tatttggatt attagttttt taggtttagg gattatttcc ttattccaac aagttgcagc
      901 tggtgtaatg tctatgggag cacctacttg ggatataaca gaagtattgg ctaatttagg
      961 ttttggatgg gtagcatttg ttattttaat acttgcatct tggtcaacaa acgtatcaaa
     1021 tgcttattct ggtggattag cactaaaaac tatttttcca aatgtaaaaa gaaatgtttt
     1081 gactttagtt gcaggtctaa taggtacgat aatagcagct actggtataa tatttaaatt
     1141 tcagtcattt ttatctttct tgggaattgc agttcctgca atagcaggaa ttatgtggtg
     1201 tgagtattat tttatacaag gaagaactta taagcataga gaaggaataa attggattgc
     1261 agtaataagc tggttgatag gctttgcagc gtcgtattat agttcaaaaa taaatttctt
     1321 aatacctcca ataaatggaa ttgtagtgag tatggttatt tattatgtat taatgaaatg
     1381 ttttggaatc aaagataaat aaaggaggca attacatggg tcgtaaggtg aaaataggta
     1441 taattcaaca acacagtgta cttggagatg ttaaaaaaaa tatagaaaaa gcagtagaga
     1501 tgatagatga cttaggaaaa caaggggcag atataatttg tttaccagaa ctatttgcta
     1561 cagaatataa tttagagtct ttaggtggag ttaaaacatt agaattaata agagaacata
     1621 ataagtacat agagaaatca atgtcagaag ctgctaaaag aaataatgta tatctgatat
     1681 ctccatatgg aaccttagaa aaagggtcta cacacgttta taattcagca gttatattca
     1741 atagaaaagg taagattatg ggagaatact gcaaaaatca tctatggtca ttagaagcag
     1801 tatactttaa agtgggggaa aatgtagagg tatatgaggc tgattttggt agatttggtg
     1861 taatgatatg ttatgatgca ggttttccag aagtatccag agaattgaca ttaaaaggct
     1921 ctgaaataat atttatacca tctgcatgga gaatacaaga tgaagatatg tgggatttaa
     1981 atgtatctca aagagcttta gagaatactg tatatacagt aggggtaaat ttagtaagta
     2041 atgactcaaa tttaatacta tttggaaaga gtaaaatatg caatcctaga ggtactgtaa
     2101 taactcaatt agacacgtat agggaaaaat atgtattatc agagattgat ttagatgaaa
     2161 taaaaaagta tagggaagat atcccatatt tgaaagacag aaaaagtaac tataacatat
     2221 aattctatat tataatacaa gttcaatata aaatgacaaa gagaggatgt atcaatatta
     2281 agattaaatt tattatactt tgatatatcc tctttaatat tatcaacttc gatttaaata
     2341 ttcttaaaat agttgataag aaagtttttg aataacaata cagctttaga ttcatagttt
     2401 tcactaaacc atgaaagatt taaatatctt ttaaattcaa tatctttgat atgtaacaac
     2461 ttaatctcag ggtcttgaga taatccccat gattttccac atataaatcc aactccttga
     2521 agtgatttta taagagcata aattgtatat ggcgaatcag attcaaatgc tatttttggt
     2581 ttaaaattag ctgattcgca taggatatca attacttctc tataattctc acccttcgtt
     2641 ataactataa agttttcatc tgaaacttca cttagatata cttcatcctt taatgacaaa
     2701 ggatggttta ttgaaactcc taatagtatt tcttcgcatg ttaaagttat gctattttct
     2761 gagtttagct ttgtaaatga agaagatatg tacaaatcaa aatcactttt tttataagat
     2821 gatggcaaag tatgtgacac attaaaagta atattgggat atagttttct gaaattactc
     2881 aaaagaggtg gtaacacatt agccgctgca ggagataata tctttacttc tcctgtattt
     2941 tctaaagaag tgtcttgtaa ctcctttttt gaactttcta ccaaatctaa aatgctatct
     3001 actcttttta gaaacaatct tccatactca tttaaaacaa tgtatcttcc ttttctatca
     3061 aataactgaa ctccaagttc cttttctaag gaagagatag ttttgcttaa agctggttgt
     3121 gctatattta atttgtgtga tgcttttgtt atgtgctcca ttctagcaac agtttgaaaa
     3181 tacttaagat gcaataaatc catagtataa aatacacctc ttttaatatg actttagtta
     3241 tatcaatttt ataatcaact ccacattata cattataata taaagtttgt ataataaacc
     3301 acaatatatt aaaaagttat ttaaattatc atttatattt tgtacataat ctaattaaaa
     3361 aagtagcttt aatatgatta actaaggaaa cattattaca aagcttaatt acacaaaaaa
     3421 ttcatttgat gagtaaagta ttaatgtcag gtaagaataa aagcttattg gagaagaaaa
     3481 ataatatttt ataataatga tattacttaa attgttgtgt tatactattt taaataggaa
     3541 acatttagat actataattt tagggggagt caagatgaaa ttaactaaat caataccatc
     3601 aatgctgtgc ttatctatag ttttgagctt atcaccagta tcatatgcaa atgaaataag
     3661 tagaacaaca gttgagaata ctttagtagg tcaggataga tatcaaactg ctgttgaagt
     3721 aagcaaaaaa ggatggagta gtgcaaatga ggctgtagtt gtaaatagtg aatctattgt
     3781 agatgcattg gctgtaacac cgtttgctaa attaaaaaat gctcctatat tattgacaga
     3841 aaaaaataat ctaaattcac aaactgagaa agaattaaaa agattagggg ttaaaaaagt
     3901 ttatataatt ggactatctg gttctgtatc aaatgatgtg caaaaacagt tggaatctaa
     3961 taatttaact actgatagag ttggtggtag tgatagatat caaacagctt taagtatagc
     4021 taaaaaaata gaagaattaa aagatatatc agaaatagct atagtaaatg ggtacacagg
     4081 tcttgcagac gcagttagta tagcttctgt agctgctact aatggaatgg ttatacttcc
     4141 agtgtctgat gcatctggta tatctagttt taaaaatttt atagaatcta agaatataag
     4201 taaatcatac ataataggat ctactaatgc tgtatcagat aaaataaaac aaagtttacc
     4261 taattcagaa agaataggtg gagcagatag aaatgaaact aatggtaaag taatagaaaa
     4321 gttttataca tctaacaaat taaataatgt atttgtagct aaagatggaa tgaaaaagca
     4381 aaatcaactt atagatgctt tagcagtagg agtactggca tcaaaagaaa attcacctgt
     4441 actaatcgta ggagagcaat taagtgcaac acaaaagagt gtattttcga ataaaaatac
     4501 taccgctata actcaagttg gtagtggtgg aaatgaaaat gcatttaaag aattaaaaga
     4561 tttacaggta aatagtaata ataagagagt aatgtatgta actaatacag ataaggtaaa
     4621 tataagaagt gatgctacaa tagaagcttc tgttatagga gctctaaata atggtgatga
     4681 agtagaagtt ttagaagtgt taaaaactgg atgggtaaaa ataaaataca atgaaggtat
     4741 tggatatgtt agtggctctt atttgacaaa taataagccc gataatagta atgaaaatat
     4801 aaaaataaag tatgtaaaag aaaaagatgg tctaaatgtt aggaaaggac caagtacaga
     4861 agatgaaaaa atagggcatc tttcatatgg aagcaaagtg gaaacaatag aaatgtttgc
     4921 tactggatgg gtaaaaataa aatataatgg tggatatggt tatgtaagta atgattactt
     4981 atctgataca ccagttattt aattataagt aacaataaaa aactgcttta atttgtaaag
     5041 cagtttcttt tttgtaaaat tttgagtcta ataattattt ctattttgat accttaattc
     5101 taggatttat tattatggat attagaaaca tacaaacact ccaaatccag tgcctaattc
     5161 caaacttaga tattaataaa ttcagtcctg ctatagtaaa taagcagtat ttaattacaa
     5221 gtaaaagtgt atttatagaa attcctttta tagaaagtat cctaaatcca ataatagaca
     5281 ttctaaatcc tataaatgcc aataatatac ctgtaatgtc ctgaattaag taatagtatt
     5341 caaatctcat atttaatcaa ctcttttctg aattgtgtca aatatttcta actcttcaat
     5401 ttcattatct gtatatgtaa gcatagtaaa accagttaca ccataaataa atgaaattat
     5461 aggagtaatc caacttaaga aacaatatgg aataaatttt aatgggctta caccaagagt
     5521 ttgagcacag aatacagcat ttgaattcca tggtattaat ggaccaccaa gcgtagctgt
     5581 atcttctata attctagata gattctgtgg ttttaattta aattctttgt atattggaga
     5641 cattagagtt cctacaaata cagaagtaaa catcattgac gaactaaatg catttgcaaa
     5701 gtagcttact ataaaagtag aaccaaccaa tttagttctt gattttccta ttttatttat
     5761 aagaggttga aggagtactt ctaagaatcc agttttttga agaataccag caataacaaa
     5821 gactacaaat actagtaaaa cggtttcagc catactcatt attccaccac gatttaatag
     5881 tttgtcagca tagacaaatc cagtatctat agtaaatcct gatagcatgt aatttaaaac
     5941 atcacctatt ttttctcctt cttgaaatac tgctataata agtcccatga tagctgaact
     6001 tagtatagat atgataggtg gcttttgtag caataaaagt aataataaaa atattattgg
     6061 aatcattgct actaaaccaa tatgaaagtt tgaatttaat acatctttga tttgatttat
     6121 ttgaacgtag tctatagtat tatttgaata cttaaatcca attactgtat acagaactac
     6181 acatattaca tatgcaggtc ctgtggtata aaacatatgt ttcatatgtg taattacatc
     6241 tgtcttacaa actgccgcag ctaagtttgt agaatctgat agaggtgaca tcttatctcc
     6301 aaagaaagca ccagaaatga ctgcaccagc tgttaaacca aggggaatac ccatactagt
     6361 accgatactc atcattgcta taccagaagt tccaagagtt ccccaagatg taccagtagc
     6421 aacagaagtc agtgaacata cgattagtgt tgtaagcaga aaatatttag gggttattat
     6481 tgttagtcca ctatatacta ctgttggtat tgtaccagca gctatccaag ctgctattag
     6541 tacaccaaca gaaagtataa ttaagtttga ttgaaaggca tctttaccaa cctcaaatcc
     6601 aaaagcttct atttcatcat tagtataacc aagcttcata gctgcaggaa ctactataag
     6661 ccatgaaagt aaaaacattg tagttataga agcattaaat acaactatgc caactgatgt
     6721 tagagctatt attgcaagca ttactaacaa ggaatatcta aatgaaggta tttttctttc
     6781 tgtattcatt ttcatagtgt aaattctcct taaatatttt tagttaattg cattttttat
     6841 tcttctaaga gcttcttcaa gcatgccctt tggacaagct aggtttattc tttgataccc
     6901 actgcctcca atgccaaagc tatttccttg atttagagca actttacctt tctgtacaag
     6961 tttactttct agttccttat cacttaatcc aagtgcgcta aagtctaccc ataataaata
     7021 agtgccttct ggttttctaa ctttaagttt tggcatattt tcatttatgt attttatagc
     7081 aaaatctata ttagattcta aatattctaa aaatgattct aaccaaggtt caccattatt
     7141 atatgaaact tctgttgcaa ctaagctaaa acaattattt ctctttatat caattctagt
     7201 aaaggcatca tctaacaatt tatagtcttt ttcatcaggt agtattacat atgatgattg
     7261 aagaccagct atattaaaag ttttagttgg agccatacaa gtaattgtat ttttttcaaa
     7321 ttctttagaa atagatgcta taggaatatg cttatgtttt tttaatataa tgtcactatg
     7381 tatttcatca gatatgattt ttacattatg cttaagacaa atatctccta gtttttttaa
     7441 ttcatctttt gtccatactc taccaactgg attatgagga ttacaaagta tgaataattt
     7501 tacatcttta attttgtttt ctatatcttc ataatccatc atatagtttc catcttctag
     7561 tttttgtaat gggcttataa ctagttctct actattattt tttacaacac tattaaaagg
     7621 actgtaaact ggctcttgta tcattatttt atcattttct tttgtgagct cgtttattaa
     7681 aagacttata gctggtataa cacctggact ataaattaac cactcacttt ttattttcca
     7741 attatgtctt ctatctaacc aactcactat agattcattg taagaatctg gtcttgttgt
     7801 gtaaccataa atttcttgct ctattctatt ttttaaggaa tcaattatac aaggtgcagc
     7861 tttaaaatcc atgtcagcta cccacatagg aagtaaatca ttagttccat actttttttc
     7921 catctctgac catttagatg aaaaattatt gcttctatca acaatttcat taaaattata
     7981 attcattgta aatacctcca gtaattttaa ttttatgtta ataatattaa gcaaacaacg
     8041 tgccaagatt gataataaaa aatatttatt cacaagttaa tatttaatat taagttattt
     8101 cagcgtatag ataagataat aatattaatt atatttaaaa ctttgtggaa aaggttattt
     8161 ctaataaatt ttcaaatata tgaaaatcca attaaatagg tttaataggt ttaactgttt
     8221 aacttattag acctgtttat attttttaga aattcaactc cttttgatgt tataattgaa
     8281 ccaccacgac caacaagtat gtttacaagc ccaaattctt gtagttctag aagtatattt
     8341 ctaatctgtt gttccgtcaa aaatctattt tcctctaatg ctattttata taagcttttt
     8401 ctgcctattc tttcttttaa atcataagcc ttttttaaat tatccagtat aaatatgtat
     8461 tcatctagtg accttttaaa attatagtcg tatttcataa gatttttgtc ttt
//
LOCUS       DAFNNB010000154         8511 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0159, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000154 DAFNNB010000000
VERSION     DAFNNB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0159"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..892)
                     /locus_tag="L3M60_003023"
     CDS             complement(<1..892)
                     /locus_tag="L3M60_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227657.1"
                     /translation="MNDKNTYFDRKGKENFIDIFFKDDIKKGANFKRHWHEHLQVYYF
                     VDGIAYIECGLNNFKIVSGNIAIANSNELHYLESLSDNLKFYIIRIEPTFLFSNQVDL
                     LQTKYLAPLALNHIVFNNLIQNDSFILKCIKTILDEYHNKGIGYELSIKANIYQLIVL
                     LLRSHVDKIFSEDEFIERKYSLEKFEYIFDFIEEKYMDKITLKELASHVNLSVPHFCR
                     TFKKITGKTTIDFINGIRLDKAIYYLKETDLNMTEIAMRCGFDSINYFSRLFKRNYDV
                     SPTKYRNEHREISNVILKEGI"
     gene            1034..2461
                     /locus_tag="L3M60_003024"
     CDS             1034..2461
                     /locus_tag="L3M60_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBN6227658.1"
                     /translation="MTNSFKKDENKKRMIVLFVVIAMTFMATLDSSIINVALPVLASK
                     LNVSLSSIEWVIASYSIIICSTLLFFGRLGDIIDKSRVFQSGTILFTSASLLCGLSNS
                     LTLLIVCRFIQGIGASAYMANNHGIITELFPKENRGKALGILVTAVAIGNMVGPSIGG
                     FILSIFDWNFIFFINIPIGIIVIFLNTKFLPNSKKSSENMNKTGAILQFLGTTLFFSA
                     LISAQQAGLLNPYILIALLLSIIFIILFLILEKKHPQPLLDLEIFRNFKFSLNLICAL
                     TSFICIASSSILIPFYLQNTMKLAPIQAGSFMILSPLILAIFSPIFGNISDKIKSEKI
                     ILIGLLFMSFGFFLMSRLKESSALILFVIYISIISMGQAIFQPANNALIMSSCYRSKL
                     GIVGSINSLVRNLGQVIGITISTTLLYNFMSIKAGYRVNDYIINNDKIFVFGMRNVYT
                     IITLICLIGAILIGFYLFKYDKNKQ"
     gene            complement(2638..5232)
                     /gene="clpB"
                     /locus_tag="L3M60_003025"
     CDS             complement(2638..5232)
                     /gene="clpB"
                     /locus_tag="L3M60_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBN6227659.1"
                     /translation="MDVEKMTLRVQKSLNEAYNEAVKNHNQQVDVIHLFSALINQEDG
                     LIPNIIEKMNISIDSLRNTVNFEIDKLPKVYGEGADSQGVSATRKINEVLIKAESISK
                     EFKDSYISVEHVMLAMMETESKSAVGKILKQYNINKNDFLNILSQVRGSQRVETQDPE
                     GTYDALARYGTNLVDLAKKNKLDPVIGRDEEIRRIIRILSRRTKNNPVLIGEPGVGKT
                     AIVEGLAERIVRGDVPEGLKDKIVFSLDMGALIAGAKYRGEFEERLKAVLKEVQSSDG
                     KIILFIDEIHTIVGAGKTEGSMDAGNLIKPMLARGELNCIGATTFDEYRKYIEKDKAL
                     ERRFQPVIAEEPTVEDTISILRGLKERFEIHHGVRIHDNAIVAAAKLSNRYIQDRFLP
                     DKAIDLIDEAGAMIRSEIDSLPTELDVVRRKLFTLETEREALLKENDDKSKSRLDDIQ
                     KEIAELKSKNDEMTAKYEKEKSQILDIKNLKAQLDEAKGKAEKYEREYDFNKAAEVKY
                     GEIPKLEEQIKQYEENMNDGSENSLLKEEVTEEEISSIVSKWTGIPVTKLVEGEREKL
                     LKLEDELHKRVIGQDEAVTAVSNAVIRARAGLKDERKPIGSFIFLGPTGVGKTELAKT
                     LARNLFDSEDNIIRIDMSEYMEKHAVSRLVGPPPGYVGYEEGGQLTEAVRRAPYSVIL
                     FDEIEKAHEDVFNMFLQILDDGRLTDNKGKTVDFKNTLIIMTSNIGSNYLLEAGGNIT
                     ETTNNLVMNEMKHRFKPEFLNRVDDIIMFKPLDQENIKKIIDIFMKDLKNRLKEKDIT
                     IEVTNSAKDVMVREGYDPVYGARPLKRYIGNTLETVIAKKLIAGDVYNGCTIVIDGKD
                     ENIEVLVK"
     gene            complement(5269..5865)
                     /locus_tag="L3M60_003026"
     CDS             complement(5269..5865)
                     /locus_tag="L3M60_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227660.1"
                     /translation="MSLKLDRNVLQWFDYVFENEKTSLRHYNFNCTLKEISSTSLNKV
                     AFILEKNNSKYWKLYFEIPAEVTLKLKQNIHPLFREYIYEQISLYNNNQIYNFVNSNI
                     LKVFNNIAIYQYNILENLYTIDFKKSFIDKCQYLLIGEKRLIDEDLYLIVKSKEVFDF
                     FNSDGTFNLTLSFDIQKNENLLDSLLELRKSIIINERI"
     gene            complement(5882..6286)
                     /locus_tag="L3M60_003027"
     CDS             complement(5882..6286)
                     /locus_tag="L3M60_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBN6227661.1"
                     /translation="MDKTLKKDLNQEYTESKSLVMNKSKYMNVCFKNYIKVVEGMYTK
                     RDLIPLKLINSMDKNNKIRKTLYELSCEFEYPKTSLSSLFTELKKMDFLQRVKNGEYM
                     INPHIAYKGSRADKESLLAEYNQIKRPNKVSK"
     gene            6653..7027
                     /locus_tag="L3M60_003028"
     CDS             6653..7027
                     /locus_tag="L3M60_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBN6227662.1"
                     /translation="MNIVISNSSGKPIYEQITTQIKSMIISGELPEGSALPSMRLLAK
                     ELRISVITTKRAYSDLERDGFIETVTGKGSFVSSKNMDFVKEEQFRLIEEYLQKAVDT
                     AKSSDITYDELKDILFLLYKGE"
     gene            7024..7887
                     /locus_tag="L3M60_003029"
     CDS             7024..7887
                     /locus_tag="L3M60_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227663.1"
                     /translation="MISMDAITIKNLNKTYKDFSLQDISFSVPKGSVMGFVGENGAGK
                     TTTLKAILNLISYDSGNIEIFGLDSKKNEKEIKEQIGVVFEGSNFHENLNTDHVSKIM
                     SKIYKNWSDTLFKDYLKKLRVPDNKLIKEFSKGNKMKLSIAVALSHNPKLLILDEATS
                     SLDPIVREEILDIFLDFIQDEEHSIILSSHITSDLDKIADYITFIHKGKIVFSENKDE
                     LIDTMGVLKCKPSDFDNLSREDYSYYRKSQFGYEVLLKDKHRFISRHPNCIVDNTSIE
                     EIMLFYVRGDK"
     gene            7887..>8511
                     /locus_tag="L3M60_003030"
     CDS             7887..>8511
                     /locus_tag="L3M60_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HBN6227664.1"
                     /translation="MKGLILKDLLNLKGNVKFILLFIIMFGFMSSLGDGNINNFIGVI
                     IVLCTTMIVSTFSYDDLNKWDSYVLTMPINRNDIVLSKYLTMLIFSFIGVLVSLIVSV
                     TIGYFKNTLILNETLLINALILSISVCFGSLILPLIYKFGTERARLLMILCFLVPTLA
                     LLVFKSILENISSPISIEIILNTLVYSLPFVAILLFVISYFISSKIYS"
BASE COUNT         2856 a         1273 c          985 g         3397 t
ORIGIN      
        1 taataccttc ttttaaaata acattactaa tttctctatg ttcatttcta tacttagttg
       61 gagatacatc ataattcctt ttaaataagc gactaaaata atttatgcta tcaaagccgc
      121 atctcatagc aatttctgtc atatttaaat ctgtttcctt taaataatat atagctttat
      181 caagtctgat tccatttata aaatcgattg tagtcttacc tgttattttc ttaaatgttc
      241 tacaaaaatg aggcacactc aagtttacat gactagcaag ttcttttaat gtaattttat
      301 ccatatattt ttcttctata aaatcaaata tatattcaaa tttttctaaa gaatactttc
      361 tctctataaa ttcatcttca gaaaatattt tatcaacatg acttcttagt aataatacta
      421 tcaattgata gatattagct tttatagata gctcatatcc aattccttta ttgtgatatt
      481 cgtcaagtat agttttaata cattttaaaa taaaagaatc attttgaatt aaattattaa
      541 atacaatatg atttaaagct aatggtgcta agtacttagt ttgtaacaaa tcaacttgat
      601 tactaaaaag gaatgtgggt tcaattcgaa taatatagaa ttttaaatta tctgataaac
      661 tttctaaata atgcaattca ttactatttg ctattgcaat atttccagat actatcttaa
      721 aattattcaa tccacattca atataggcta taccatcaac aaaataatat acttgtaaat
      781 gctcatgcca atgtctttta aaatttgctc cctttttaat gtcatcctta aaaaaaatat
      841 ctataaagtt ttctttacct ttcctgtcaa aatatgtgtt tttatcattc attatttacc
      901 tcttatcaaa atagtgctaa ttatgagcaa tatcgttatt gtattcatta tttcataccg
      961 atattatata agatgtgact attattgtaa acattaacat gatgccagtc attataataa
     1021 ggaggtaata aaaatgacaa actcttttaa aaaagatgaa aataaaaaaa gaatgatagt
     1081 tttatttgta gtcattgcaa tgacctttat ggcaacatta gattctagta ttataaatgt
     1141 tgctcttcca gttcttgcaa gcaaactaaa tgtatctcta tcatctattg aatgggtaat
     1201 agcaagttac tcgattatta tttgttcaac actattattt tttggaagac ttggggatat
     1261 tattgataaa tccagagtat ttcaatcagg tactattctt tttacatcag cttcattatt
     1321 atgtggatta agtaattctt taactttact tatagtttgt cgctttattc aagggatagg
     1381 tgcttctgcc tacatggcaa acaatcatgg tattatcaca gaactgtttc caaaagaaaa
     1441 cagaggaaag gcactaggaa ttttagttac tgcagttgct attggaaata tggttggacc
     1501 atctatagga ggatttattt tgtcaatatt tgattggaat tttatattct ttataaatat
     1561 tccaatcggt attattgtaa tctttttaaa tacaaaattt cttcctaata gtaaaaaatc
     1621 ttcagaaaat atgaataaga ctggtgctat acttcaattt ttaggaacaa cattattttt
     1681 tagtgctcta attagtgctc aacaagcagg attactaaat ccatatatat taattgcact
     1741 attattatca attattttta ttatattatt tttgatatta gaaaaaaaac atcctcaacc
     1801 gcttcttgac cttgaaattt ttagaaattt taaattttca ttgaatttaa tttgcgctct
     1861 tacatcattc atttgtattg catcatctag tattttaatt ccattttatt tacaaaatac
     1921 tatgaaactt gcccctattc aagcaggttc atttatgata ttatcacctt tgattttagc
     1981 cattttttca cctatatttg ggaatatatc agataaaatt aagtcagaaa aaattatttt
     2041 aattggtctt ctctttatga gttttggatt ttttctaatg tcaagattaa aagaaagttc
     2101 tgcccttatt ttatttgtaa tttatatatc gataatatca atgggacaag ctatatttca
     2161 acctgctaac aatgctttga taatgtcaag ctgttataga agtaagttag gaattgtagg
     2221 aagcataaat tcccttgtaa gaaatttagg acaagttata ggtattacta tttctacaac
     2281 acttttatat aattttatga gtattaaagc tggatatagg gtcaatgatt atataattaa
     2341 taatgataaa atatttgtat ttggaatgag aaatgtttat actattatta cacttatttg
     2401 cctaataggt gcaatattaa ttggctttta tttatttaaa tatgataaaa ataaacaata
     2461 gtttttttat aattgtattc aaaccattat tatattataa gaaacttact aaaatactcc
     2521 ctttgagtta aatttaattt gaacacattg gcttaaaaat atttcttttc ctatagttta
     2581 taaataaaaa tcagctgagc aaaatatttc atatactatg ctcagctgat tctcttatta
     2641 tttaactaaa acttcaatat tttcatcttt tccatctata actatagtac atccattata
     2701 cacatctcca gcaataagtt tctttgcaat tacagtttct aatgtatttc ctatatatct
     2761 cttaagaggt cttgctccgt atacagggtc atatccctct ctcaccataa catcttttgc
     2821 tgaatttgta acttcaatag taatatcttt ttcttttaat ctatttttta aatctttcat
     2881 aaatatatct ataattttct tgatattttc ttggtctaaa ggcttaaaca ttataatatc
     2941 atctactcta tttaaaaact caggtttaaa tctatgcttc atttcattca ttactaaatt
     3001 attagtagtc tctgttatat tccctcctgc ttctaataga taattacttc ctatattaga
     3061 agtcattatt ataagagtat ttttaaagtc tactgtctta cccttattat cagtaagcct
     3121 accatcatct aatatctgta agaacatgtt aaatacatcc tcatgtgctt tttctatctc
     3181 atcaaataat attacagaat aaggagcacg tctaactgct tctgtaagtt gaccaccttc
     3241 ttcatatccc acataccctg gaggaggccc aactagtctt gatacagcat gtttttccat
     3301 gtactcagac atatcaattc tgattatatt atcttcacta tcaaataaat ttctagcaag
     3361 agtttttgca agctcagttt taccaacacc tgtaggacct aagaaaataa atgaaccaat
     3421 tggttttctt tcatctttta atcccgctct tgcacgtatt actgcatttg aaactgctgt
     3481 aactgcttca tcttgtccaa taactctttt atgaagttca tcttctagtt taagtaattt
     3541 ttctctttct ccttcaacta gtttagttac tggtatacct gtccactttg atacaatgct
     3601 agatatttcc tcttcagtaa cttcttcttt taaaagagaa ttttcagaac catcgttcat
     3661 attttcttcg tactgcttta tttgttcttc taacttaggt atttcaccat atttaacctc
     3721 tgctgcttta ttaaaatcat attctctctc atatttttca gctttacctt tagcttcgtc
     3781 taattgagcc tttaagtttt taatatctaa tatttgactc ttttcctttt catattttgc
     3841 tgtcatttca tcatttttag attttaactc tgctatttct ttttgaatat catccaatct
     3901 tgatttactt ttatcatcat tttctttaag taatgcttcc ctttcagttt ccaatgtaaa
     3961 taattttctt ctaacaacat ctaactctgt tggtaatgaa tctatttcgc tacgaatcat
     4021 agctccagct tcatcaatta agtcaattgc tttatctggt aaaaatctat cttgtatata
     4081 tctattagaa agttttgctg ccgctacaat agcattatca tgtattctta ctccatggtg
     4141 tatctcaaat ctttctttta aacctctaag tatagatatt gtatcttcaa cagtaggttc
     4201 ctctgcaatt acaggttgaa aacgtctctc aagagcctta tctttttcta tatactttct
     4261 atattcatca aaagtagttg caccaataca gtttaattca cctcttgcaa gcataggctt
     4321 aataagattg cctgcatcca ttgagccttc tgttttacca gctccaacta tagtatgaat
     4381 ctcatctata aatagtataa tttttccatc agaactttga acttctttta agactgcctt
     4441 taatctttct tcaaattcac ctctatattt tgcaccagca attaaagcac ccatatcaag
     4501 tgagaataca attttatctt ttaaaccttc tgggacatct cctcttacaa ttctctctgc
     4561 taaaccttct acaattgcag ttttacctac accaggctca ccaattagca caggattatt
     4621 tttagttctt cttgataaaa ttcttataat tcttcttatt tcttcatctc ttccaattac
     4681 agggtctaat ttatttttct tggctaaatc tactaagttt gttccatatc tagcaagtgc
     4741 atcatatgtt ccttctgggt cttgtgtttc tactctttga ctacctctca cttgtgataa
     4801 aatgtttaaa aagtcattct tatttatatt atattgcttt aatattttac caacagctga
     4861 ttttgattct gtttccatca ttgcaagcat aacatgttct acacttatat atgagtcttt
     4921 aaattcttta gaaatgctct ctgcctttat aagcacctca tttatttttc tagtggctga
     4981 tactccttgt gagtcagctc cttctccata tacttttgga agcttatcaa tttcaaagtt
     5041 tacagtattt ctcaaagaat caattgatat attcattttt tcgattatat ttggtattaa
     5101 tccatcttct tgattaataa gtgctgagaa aagatgaatt acatctactt gttggttatg
     5161 attttttact gcttcattat aagcctcatt taaacttttt tgaactctta aagtcatttt
     5221 ttctacgtcc atataaatcc ctccatttta atattatatc caaataaatt aaattctctc
     5281 atttataatt attgattttc taagctctaa aagactatca agtaaattct catttttttg
     5341 tatatcgaaa ctcaaagtta aattaaatgt tccatctgaa ttaaaaaaat caaatacttc
     5401 ttttgatttt actattaaat acaaatcttc atctataagt cttttttcac ctattaataa
     5461 atactgacat ttatctataa agctcttttt aaaatcaata gtgtatagat tttctaaaat
     5521 attatattga taaatggcaa tattgttaaa cacttttaat atgtttgaat ttacaaaatt
     5581 gtaaatttga ttattgttat acagtgatat ttgttcgtat atatattccc taaataaagg
     5641 atgaatgttt tgcttaagtt ttaaagtaac ttcagctggt atttcaaaat aaagcttcca
     5701 gtacttagag ttattttttt ctaaaataaa agctacttta ttaagactgg tagaactaat
     5761 ctcctttagt gtacaattaa aattatagtg tctcaaacta gttttttcat tctcaaatac
     5821 ataatcaaac cactgtagaa catttctatc aagttttaaa ctcatataaa aaccttcttc
     5881 cttatttact aaccttatta ggtcttttta tctgattata ttctgctaat aaactttctt
     5941 tatcagctct actaccttta tatgctatat gtggatttat catatactca ccatttttca
     6001 ctctttgtaa aaaatccatt tttttcagct ccgtaaacaa tgaagataaa gaagtttttg
     6061 gatattcaaa ttcacaagat aattcatata atgtttttct aatcttatta tttttatcca
     6121 tagagtttat taattttaat ggaattaaat ccctcttagt atacattcct tctactactt
     6181 taatataatt cttaaaacaa acattcatat acttactttt attcataact aaacttttac
     6241 tctctgtgta ttcctgattt aaatcctttt ttaatgtctt atccaaatta aatatctctt
     6301 ctcactctcc ttatctctaa ttgataatat aaatatatta caaaaaaata tgtatgtcaa
     6361 aactgaaaac acaaaaagtt tagcttataa ataaactttt atttacctat aagctaaact
     6421 ttattatttt tttaatttag tatttccaat ttattggcag gatttttgaa taatatttat
     6481 aaatttttta aaaaaattta aatctattta atatggtagc ctttcataaa atacattaca
     6541 tttattaaat gtataatatt ggcaattagt tttttctaaa atcagttgac atattatata
     6601 tatatttata taatgtgtat atactatata tacaatttaa ggaggtgtct tattgaatat
     6661 agttattagt aattcgagtg ggaagccaat atatgaacag attactacac aaattaaaag
     6721 tatgattata agtggagaat taccagaagg ctcagctctt ccaagcatga gactattggc
     6781 aaaagaactt agaataagtg taattactac aaaacgagca tatagcgatt tagaacgaga
     6841 cggatttatt gagacagtta ctggtaaagg cagttttgta tctagtaaaa acatggattt
     6901 tgtcaaagaa gaacaatttc gtttaattga agaataccta cagaaagcag ttgatacagc
     6961 aaaaagtagt gatattacat atgacgaatt aaaagatatt ttatttttat tatataaagg
     7021 agaatgataa gtatggatgc aataactata aaaaatttaa ataaaactta taaagatttt
     7081 agcctacaag atatatcttt ttcagtacca aaagggagtg taatgggatt tgtaggtgaa
     7141 aatggtgcag gaaaaacaac tacattaaaa gcaattttaa atttaatatc ttatgatagt
     7201 ggaaatatag aaatctttgg tcttgatagt aaaaaaaatg aaaaagaaat taaagagcaa
     7261 attggggttg tatttgaggg aagcaacttt catgaaaatt taaacactga ccatgtttca
     7321 aaaatcatga gtaaaatcta taaaaattgg agtgacacat tatttaaaga ctacttaaaa
     7381 aaattgagag taccagacaa taaattaatt aaggagtttt caaaggggaa taagatgaaa
     7441 cttagtattg ctgtagcctt atctcataac ccaaaattat taatactaga tgaggcaact
     7501 agttcacttg accctatcgt tcgtgaagaa atactagata tatttttaga ttttatacaa
     7561 gatgaagaac actctattat cttatcttca catattacaa gtgatttaga taagatagca
     7621 gactatatta catttataca taaaggaaaa attgtattta gtgaaaataa ggatgaatta
     7681 attgatacta tgggagtttt aaaatgtaaa cctagtgatt ttgataacct ttcaagagag
     7741 gattattcat attatcgtaa aagccaattt ggatatgaag tacttcttaa agataagcat
     7801 agatttatct caagacatcc aaactgtatt gttgataata catctataga agaaattatg
     7861 ttattttatg tgagaggtga taaataatga aaggtctaat tttaaaagat ttacttaatt
     7921 tgaaaggaaa tgtaaagttt atacttttat tcatcattat gtttggtttt atgtcttctt
     7981 taggagatgg aaatattaat aactttatag gtgttattat agttttatgt actacaatga
     8041 ttgtttcaac attttcatat gatgatttaa ataaatggga tagttatgta cttacaatgc
     8101 ctattaatcg caatgatatt gtattaagta aatatttaac tatgttaata tttagcttca
     8161 taggtgttct cgtttcactt attgttagtg taactattgg ttattttaaa aatacactta
     8221 tattaaatga aacactactt attaatgcac taatcttatc aatttcagta tgttttggta
     8281 gccttatttt gccacttatt tataaatttg gtacagaaag agctcgttta ttaatgattt
     8341 tatgcttttt agttccaaca ctagctttat tagtttttaa aagtatttta gaaaatatta
     8401 gttcacctat ttctatagaa attatactta atacactagt atattctctt ccatttgttg
     8461 ctattttact atttgtgatt tcatatttta tatcatcaaa aatctatagt a
//
LOCUS       DAFNNB010000155         8294 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0160, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000155 DAFNNB010000000
VERSION     DAFNNB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0160"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..852
                     /locus_tag="L3M60_003031"
     CDS             <1..852
                     /locus_tag="L3M60_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBN6227665.1"
                     /translation="ELLKSSKFEHFNINQRLLSDEWILVNKEDETFYNKIQEKCKYSL
                     EDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINKYVVDKSEYRLIY
                     SNDIDNENTNKKILDEIIGIYKTKLENRRECKSGIRKWYELQWGREKLFFERKKIMYP
                     YKSNENRFAIDYDNNFSSADVYSFFIKEEYLGIFSYEYLVGILNSSIYDKYFKITAKK
                     MSKNIYDYYPNKVMKIRIFRDNNYDEIENLSKQIISILLNKSIDKGKVEKLQIKMDNL
                     IMDSLGI"
     gene            1084..2295
                     /locus_tag="L3M60_003032"
     CDS             1084..2295
                     /locus_tag="L3M60_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBN6227666.1"
                     /translation="MKGLVLEGGGTKGAYQIGAYKALRELGIEFQGVTGTSIGALNGA
                     YIIQNDIEVMEDIWLNYDYTHFMNIDRETYERFKNVDFTTKNLNAVFELINKARKNEG
                     IDISPLRQLMETTLNEDIIRKSKKDFGLVTVWWDKKINPYPLYIEDIPKGRLIDYLIA
                     SSSLPIFQTNMMDDKLYLDGMFCDNMPIGLLEDKGYEDVVVIRLIDDFIGKMNLNRHQ
                     NINIKTIIPSEYLGGSLNKDRDNVEKNIRLGYLDTMKAYERCTGIKYYFDVEYKYNEE
                     YCFDKFRSMSNETIQYICNLLNIKRDVSLRTVMESIIPKLGEVLGLPKDFSYKDLFYC
                     IYEKKLEENNINRIKLYDFNKVIDVVNTNIDSNRKLASNFEIRPKISIKKNKNTKLLT
                     NCIIKDIKNQS"
     gene            2487..2747
                     /locus_tag="L3M60_003033"
     CDS             2487..2747
                     /locus_tag="L3M60_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBN6227667.1"
                     /translation="MEKVVSIKNASGLHARPAGMFVKKAAEFKSTVEVVAKDKTVNAK
                     SIMGIMSLGLAQGEELKLVVNGEDEEVALAAMVELVESGFGE"
     gene            3100..4812
                     /gene="ptsP"
                     /locus_tag="L3M60_003034"
     CDS             3100..4812
                     /gene="ptsP"
                     /locus_tag="L3M60_003034"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBN6227668.1"
                     /translation="MAYKGIGASPGVALGKALVVEHSELVIEKKSIDNVEAEIAKLEN
                     AVAVSKDELVKVKEKASEELGAEEAEIFEAHLLVLEDPELIGSAIDKIKTENVNAEYA
                     LNEIKEMFVSMFESMDNEYMKERAADIKDVTNRILRHILGIKVVDLSALSEEVVLIAH
                     DLTPSDTATMNKKMVLGFLTDIGGRTSHTAIMSRTLEIAAIVGLNDITSKVKDGDFVV
                     FNGDTGEVIVNPDKETINKYTELKAKYEDERKALQLLKGKPSVTLDGKHVELAGNIGT
                     PNDIEGLIKNDAEGVGLYRTEFLYMDRDSFPTEEVQYEAYKAVLEGMDGKPIVIRTLD
                     IGGDKELSYLNMEPEMNPFLGYRAIRLCLDRKDIFKTQLRALYRASVHGRLRIMFPMI
                     SSLEELLQAKEVVKEVLAELDSEGVAYAKDVEIGMMIEVPSAAVISDVLAKHVDFFSI
                     GTNDLIQYTCAVDRMNQKISYLYNQFNPAVLRLIKTVIDNAHKEGKWAGMCGESAGDQ
                     KMIPILLGMGLDEFSMSPISILPARKLITSVKESDMKKLADEVLNMGTAEEIKSYIEK
                     TFNI"
     gene            complement(5604..6269)
                     /locus_tag="L3M60_003035"
     CDS             complement(5604..6269)
                     /locus_tag="L3M60_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227669.1"
                     /translation="MKGKIVQYNFADIEEEVYSLDYAIAWNTDEENVNIIPFTNKFCK
                     ESIESFCLGKIDNFVEILNEGFVENHHYVHLDKMISVPKKKVNLVYQQDTHGYLLRDD
                     NGNLIPAKITSEQSKSISSKMELFCAGEEKCLINILLKADPSYILDVDSIKDKNILNL
                     GYESIDRYKEYNFDNDKILIFFINKKRYSVIMKKTNNSDNDLVSRNNAIKELFTNKAV
                     NLN"
     gene            6614..8044
                     /locus_tag="L3M60_003036"
     CDS             6614..8044
                     /locus_tag="L3M60_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4116 domain-containing protein"
                     /protein_id="HBN6227670.1"
                     /translation="MDIKNKIKSIFLPEKNDYLDDEEFEYDIENNEEDSKEDNKVHLN
                     DLSRVKYDYRELKDIENQTEEMCLLAVKQDGTIIEFVKDKTYKVCMEAVKQTYKALKY
                     ITNQNEDICIEAVKQNYRALYYINNKTENVLIEAIKNASTYDVMEVFKFVEEQTEDVC
                     LAFIERASKNDVAEILKGIKEQTPAICLEAVKKDGKSLAYVKEQSNSICLEAVKENYS
                     ALSCVKEQTEEICIEAVKQNDFALYYVNEQTEKICMEAVKRSYMALQYVNKQTEEICL
                     EAVRIDGRALQYVKEQTEEICLESVRQNGKVLQYVKKQTENICIEAVRGSFEELEIKE
                     ILSYVKIPTERIFVEAVKQNGKILKYVENQTELICLEAVRENYNALAYVKEQTEEICL
                     EAVNQSYEALKYVKEQTEEVCLKAVKQDYRMLKYVNNQTEKICLEAVKQNYRALEFVD
                     NQTEKVCLEAVKQNRKALQYVKQKQS"
BASE COUNT         3488 a          764 c         1378 g         2664 t
ORIGIN      
        1 gaacttctaa agagtagcaa attcgaacat tttaatataa atcagagatt actatctgac
       61 gagtggatac ttgtaaataa agaggatgaa actttttata ataagataca agaaaaatgt
      121 aaatacagcc tggaagatat agcgataagt tttcaaggaa taattacagg gtgtgataaa
      181 gcatttattt taagtaaaga tgatgtaaaa ttgaaccttg tagatgataa atttttaaag
      241 tgttggataa aaagtaagaa tataaataaa tatgtagtag ataaaagtga atataggcta
      301 atatactcaa atgatataga taatgagaac acaaataaaa agatactaga tgaaattata
      361 gggatatata aaacaaagtt agaaaacaga agagaatgta aaagtggaat aaggaaatgg
      421 tatgaacttc aatggggtag agaaaagctt ttcttcgaaa gaaaaaagat aatgtatcct
      481 tataaatcaa atgaaaatag atttgctata gattatgata acaatttttc aagtgcagat
      541 gtatattctt tttttataaa agaagagtat ttaggtatat tttcatatga atatctagtt
      601 ggaattttaa attcaagtat ttatgataaa tactttaaaa taactgctaa aaaaatgagt
      661 aaaaatatat atgattacta ccctaataag gttatgaaga taaggatttt tagggataat
      721 aattatgatg agatagagaa tttatcaaag caaataatat caattttatt aaataagtct
      781 atagataaag gaaaagtaga aaaattgcag ataaaaatgg ataacttgat aatggattct
      841 ttaggtatat aagcagttgt aagtgtgaaa aaattataat ttgttaattg tttatttaaa
      901 ttcacaaata aaaagttgac catataaaaa atattaaaaa tatatacttt tcaatatttt
      961 tttgataaaa taagaatgaa tttgtgcaat tttatttttc tccaataact aaaaatagtt
     1021 ttttgaatta atatttgctg aggaataaaa atcagaaacg tttttgatat agtaggtgat
     1081 aatatgaaag gattagtttt agaaggtgga ggaacaaaag gagcctacca aataggtgca
     1141 tataaagctt taagagagct tggtatagag tttcaaggag taactggcac atcaattggt
     1201 gctttaaatg gagcatatat aattcaaaat gatattgaag taatggaaga tatttggtta
     1261 aattatgact atactcattt tatgaatatt gatagagaaa cctatgagag atttaaaaat
     1321 gtagatttca ctactaaaaa tctcaatgca gtgtttgaac ttataaataa agctagaaaa
     1381 aatgaaggta tagatataag cccattaaga caactaatgg aaacaacatt aaatgaagac
     1441 ataataagaa aatctaaaaa agactttggg cttgttacgg tatggtggga taaaaagatt
     1501 aatccgtatc cattgtatat tgaagatata cctaaaggaa gattgattga ttatttaatt
     1561 gctagttcca gtttgcctat ttttcagacg aatatgatgg atgataagtt gtatcttgat
     1621 ggaatgtttt gtgataacat gccaatagga ctcttggaag ataaaggtta tgaagatgtt
     1681 gttgtaattc ggttgattga tgattttatt gggaaaatga atctaaatcg acatcaaaat
     1741 ataaatataa aaactataat accatcagaa tatctaggag gttctcttaa taaagatagg
     1801 gataatgtag aaaaaaacat tcgactaggt tacttagata ctatgaaagc atatgaaaga
     1861 tgtactggga ttaagtatta ttttgatgta gagtataaat ataatgaaga atattgtttt
     1921 gataaattta gaagtatgag taatgaaaca atacaatata tttgtaattt attgaatata
     1981 aaaagagatg tttcacttag aactgttatg gaaagtataa taccaaagct tggtgaagtt
     2041 cttggattgc caaaggattt ttcgtataaa gatttgtttt actgtatata tgaaaagaaa
     2101 cttgaagaaa ataatataaa caggataaaa ttatatgatt ttaacaaagt tatagatgtt
     2161 gtaaatacaa atattgattc taatagaaag ttagcttcta attttgaaat tagacctaaa
     2221 attagtatta aaaagaacaa aaacacaaaa ttattgacaa attgtattat aaaagacata
     2281 aaaaatcaaa gttaaataaa aaacaaaaaa aaatttgtaa ttatactcga aaaaagtgta
     2341 tattagtagt ataatatttt tgtagcacta gtgggaacaa aaaatgacca taggacaaaa
     2401 aagtaccact tagtaccatt aaagtaaaaa acaggaaaac aattttaaaa aatataaaat
     2461 gtaaagttta taggaggatt taaaaaatgg aaaaagtagt tagtatcaaa aatgcaagtg
     2521 gattacatgc tagaccagca ggaatgtttg ttaagaaggc agctgaattt aaatcaacag
     2581 tagaggttgt agcaaaagac aaaactgtaa atgcaaaatc aataatggga ataatgagtt
     2641 taggtttagc tcaaggagaa gaattaaagt tagttgtaaa tggagaagat gaagaagtag
     2701 ctttagctgc aatggttgaa ttagttgaaa gtggatttgg agaatagata taataatttt
     2761 ttaaaatttg atatttagaa tctaatatta cttaaagtaa agtaacagag aagtattaaa
     2821 catatacaga ccaacatttg tagatattta atttctttgt tactttcatg tttatttaat
     2881 cagaagatta agactattgt taattgggta taaaaataca aatattttac aaaatgaaat
     2941 aagatatttt taatcataaa ttcagaatta aaatacagta acaaatagtt tataaaattt
     3001 tataaaaaat tataagtaag taaaactata aaaatgtaaa ttatttatat aaatttatag
     3061 catttatgtg ctagatgata ttttttagga ggtagggtta tggcttacaa aggaatcggt
     3121 gcttctcctg gtgttgcttt agggaaagcg ttagtagttg aacatagtga attagtaatt
     3181 gaaaaaaaat ctatagataa tgttgaagct gaaattgcta aattagaaaa tgcagttgca
     3241 gtttctaaag atgaattagt aaaagttaag gaaaaggctt ctgaagaatt aggagcagag
     3301 gaagcagaaa tatttgaagc acatttatta gttttagaag acccagaatt aattggttct
     3361 gcaatagata aaatcaaaac tgaaaatgta aatgcagagt atgctttaaa tgaaataaaa
     3421 gagatgtttg ttagcatgtt cgagtctatg gacaatgaat acatgaaaga aagagcggca
     3481 gatataaaag atgttacaaa tagaatactt agacatatat taggaattaa agttgttgat
     3541 ttgtctgctt taagcgaaga agttgtatta atagcacatg atttaactcc atcagacaca
     3601 gcaactatga ataaaaaaat ggttttagga tttttaactg atataggtgg aagaacttct
     3661 catactgcta tcatgtctag aactttagaa atagctgcaa tcgttggatt aaatgatata
     3721 acttcaaaag ttaaggatgg agactttgta gttttcaatg gggacacagg tgaagtaatc
     3781 gtgaatcctg ataaagagac tataaataaa tatactgaat taaaagctaa atatgaagat
     3841 gagagaaaag cattacagct attaaaggga aaaccatctg taactttaga tggtaaacat
     3901 gtagaattag ctggtaacat aggaacacct aatgatatag aaggtctaat aaagaatgat
     3961 gctgaaggtg ttggtcttta tagaacagaa tttttatata tggatagaga ttctttccca
     4021 acagaagaag tacaatatga agcatacaaa gctgtattag aaggaatgga tggaaagcct
     4081 atagttataa gaacattgga tataggtgga gataaagaat taagttattt aaatatggaa
     4141 cctgaaatga atccattctt aggttacaga gcaataagac tttgcctaga tagaaaagat
     4201 atatttaaga ctcaattaag agctttatat agagctagtg ttcatggaag attaagaatc
     4261 atgttcccaa tgatttcatc tttagaagag ttgttacaag ctaaagaagt tgtaaaagaa
     4321 gttttagcag aacttgattc tgaaggtgtg gcttatgcta aagatgtaga aattggtatg
     4381 atgatagaag ttccatctgc tgctgtaata tctgatgtat tggctaaaca tgtagatttc
     4441 ttctctatag gaactaatga cttgattcaa tatacttgtg cagttgatag aatgaatcaa
     4501 aagataagtt atctttataa tcaatttaat ccagctgttt taagacttat aaagactgtt
     4561 atagacaatg cacataaaga aggaaaatgg gcaggtatgt gtggagaatc tgctggagac
     4621 caaaaaatga taccaatact tttaggtatg ggattagacg agttctctat gtctccaata
     4681 tcaatacttc ctgctagaaa gttaataact tcagttaaag agtcagatat gaaaaaatta
     4741 gctgatgagg tacttaatat gggtactgca gaagaaataa aaagctatat agaaaagact
     4801 tttaatatat aattaatgtt atatatttac taaaatttat ttttttaaat aacaaaatta
     4861 tattttacaa attaaaaagg agatgtttca aaataaaagt ttgaagcatc tcttttttat
     4921 aaaaaattac taagaattac ttaatctgtg taatagtcgt tttgtgaaaa aaacaaaaat
     4981 atattatgat tgcagtagat atggatgtta ggagaaataa aagtactatt aaaagtaaga
     5041 ttcgtcttaa aattccatta aaagcagtta agatggagta atataataat cttatgacta
     5101 taataaaaat taaaaaaata tggtattgaa aaataattaa aagtatttaa taatagaatt
     5161 agggaagaat aagtttataa gtaaaaataa ttgattagta gttaataaaa taaatgtgat
     5221 atccaaataa atcctaaatc taatttgagt tttaatatag atagtctaaa gtttgattat
     5281 taaagattta attgtaagtt gttacagttg atgatattga ttagagatta aagtttagaa
     5341 tagaaagtta ccttaaattt aaaaataaaa gataacccaa tctaaaaatt ataatcactc
     5401 gataggttta aggatttatt tgtttattat aaataagagg gtgtctcaaa agcaactttt
     5461 tagtttatgg ggcactctct tttacatgaa attaaatata ttttgttgtt tcaaaaataa
     5521 aaaagaagct tatctctatt ttgagatagc ttctttgtgt actattatta atttaatcag
     5581 taatataatt taaagttata atactagtta agatttactg ctttattagt aaataattct
     5641 ttaatagcat tgtttctgga aactaaatca ttatcagagt tgtttgtttt tttcataatc
     5701 actgaataac gttttttatt tataaaaaat attaaaatct tatcattatc aaaattgtat
     5761 tctttatatc tatcaataga ctcataacct agatttagaa tgtttttatc ttttatacta
     5821 tctacatcta aaatatatga tgggtcagcc tttagtaata tattaattaa acatttttct
     5881 tcaccagcac agaataactc catcttactt gatatagatt tagattgctc tgaagttatt
     5941 tttgcaggaa taaggttgcc attatcatct cttagaagat aaccatgtgt gtcttgttga
     6001 tatactaaat taactttttt cttaggaaca ctaatcattt tatctaaatg tacataatga
     6061 tgattttcaa caaaaccttc attcaatatt tcaacaaagt tatcaatttt acctaggcaa
     6121 aaagattcta tagattcttt acagaattta tttgtaaatg gtattatgtt tacgttttcc
     6181 tcgtctgtat tccatgctat agcataatct aatgaataca cttcttcctc tatatctgca
     6241 aaattatatt gtactatctt tcctttcaaa attactcgac ctccatctta taatatattt
     6301 aatatacttt ataaaactta tattatattt aaataatatc ataaattgga taaatatgat
     6361 atagtaaata agaaaaagtt tgaaaaaaat cactataaat gagtaataaa tataaatagt
     6421 gtatactaat gggatggaaa atgtccacta ttaactttat taccccgcat atataagata
     6481 aaattattaa tataaaaata taatctatca acaatgaata gacttttaaa gaaacttaat
     6541 ataaaattaa ttaaataagt gcttaaaaac gatttacttt aatgatagaa tcatattaaa
     6601 ggaggataat tttatggata taaagaataa aattaaatct atatttttac cagaaaaaaa
     6661 tgactattta gatgatgaag aatttgagta tgatatagaa aataatgagg aagattcaaa
     6721 ggaagataac aaagtacatt taaatgattt aagcagagta aaatatgatt atagagagtt
     6781 aaaagacata gaaaaccaaa cagaagagat gtgtctgcta gcagtaaagc aggatggtac
     6841 tataattgag tttgtaaagg acaagactta taaagtatgt atggaagcag taaaacagac
     6901 atataaagct cttaagtata taacaaatca aaatgaggat atttgtatag aagcagtaaa
     6961 acagaactac agagctttgt actacataaa taataagaca gaaaatgtac taattgaagc
     7021 tataaaaaat gcatcaactt atgatgttat ggaagtattt aaatttgtag aagagcagac
     7081 agaagatgta tgtctagcat ttatagaaag agcatctaaa aatgatgtcg cagagatact
     7141 aaaaggaata aaagagcaaa ctccagctat atgtttagaa gctgtaaaaa aagacggaaa
     7201 atctttagct tatgtaaagg aacaaagtaa tagtatatgt ttagaagctg taaaagaaaa
     7261 ctacagtgca ctgtcttgtg taaaagagca aacagaagaa atatgtatag aagcagtaaa
     7321 acagaatgac tttgcattgt attatgtaaa tgaacagaca gaaaaaatat gtatggaagc
     7381 agtaaaaaga agttatatgg ctctacagta tgtaaataag caaacagaag agatttgttt
     7441 ggaagcagta agaattgatg gaagagcatt acagtacgta aaagagcaaa cagaagaaat
     7501 ttgcttggag tcagttagac aaaatggaaa agtactgcaa tatgtaaaaa aacagactga
     7561 aaatatatgt atagaagcag taagaggttc atttgaagaa cttgaaataa aagaaatatt
     7621 aagttatgta aagataccaa cagaaagaat atttgtagaa gcagtaaagc aaaatggaaa
     7681 aattcttaaa tatgtagaaa atcagacaga attaatatgt ttagaggcag taagagaaaa
     7741 ctataatgct ttagcttatg taaaggaaca aacagaagag atttgtttag aagcagttaa
     7801 tcaaagttat gaagcgctta aatatgtgaa agaacaaaca gaagaagttt gtctaaaagc
     7861 agtaaaacaa gattatagaa tgttgaaata tgtaaataat caaaccgaaa aaatatgttt
     7921 agaagcagta aagcaaaatt atagagcttt agaatttgtt gataatcaaa cagaaaaagt
     7981 ttgccttgaa gcagtaaaac agaatagaaa ggcactacag tatgtaaaac aaaaacaaag
     8041 ttaaaaggtt aaaatatata taaaatatat gcaaacaaat aaaaattaat attttgtaat
     8101 aaataaagtg ggtatctcaa aataagcttt ttagtttgtg aggtactctt tttgtatgaa
     8161 ataaaatata tttttagtct tttaacaatg aaaaagaggg ttatctctat tttgatatat
     8221 tctttttatg catataaagc ttaataaaat tgttttatta taattgtttt attatacaaa
     8281 taataataat aaaa
//
LOCUS       DAFNNB010000156         7939 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0161, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000156 DAFNNB010000000
VERSION     DAFNNB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0161"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..230)
                     /locus_tag="L3M60_003037"
     CDS             complement(<1..230)
                     /locus_tag="L3M60_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBN6227671.1"
                     /translation="MKDDKTILILTAQFGAGHISAAKAVKESIIEKYSNYNVVIQNFI
                     NASIPMMNKPMVKLYENNTKYTPGLYNYYYYF"
     gene            429..761
                     /locus_tag="L3M60_003038"
     CDS             429..761
                     /locus_tag="L3M60_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6227672.1"
                     /translation="MNTQFRKGVLEICVLALISKKDMYGYEIVHNISKVIDVNEGTIY
                     PILRRLTKDLYFETYILESNEGPARKYYRITSLGKENLSNLMEEWREFVKAVEILISE
                     DKGGNELE"
     gene            748..1806
                     /locus_tag="L3M60_003039"
     CDS             748..1806
                     /locus_tag="L3M60_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HBN6227673.1"
                     /translation="MSLNKTEFLEILKDYLKKDFSESEVNDILRDYEEYFVDGLIEGK
                     SDMEIISSLGSPKSIAKELILQTKEKNIQEEKIINTDVLNDKFSKFKSDMKKGYSILK
                     DEAKEKYSKSKENIDGKIMSTDILNGKFSRFKSNIKKDYSILKDEAKEKYNKSKENIN
                     DKLTPSIEEGVGGLSRQVIKGLLVVLSLFLIVPAFAFVCAIVSVTIALVCSLIAFVIL
                     IPFVIKFIAMVPDVMMFCIFATIAFVGIEILAWQLLLFAIKLGKQLLNNYIHWIKRKN
                     IYIKANEKMEKRKKATLNDEDLNKDINEDSYNNDYYNREEESIKETDEYNDDSINFEA
                     IYKDLKDIENKKGEDKYE"
     gene            1799..2869
                     /locus_tag="L3M60_003040"
     CDS             1799..2869
                     /locus_tag="L3M60_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HBN6227674.1"
                     /translation="MNKKLAIFAAVLLVIGIIGTTWSGILVMPSLINFGLEKEAELNK
                     ENKLYQEKVNIDKLDVSVDNINVMIRKSSNEDVRVTTRGNNEFYKYSVTLQDKTLVVK
                     GERKSENKIKKIRSFDHLLNTSINSMFSRDYREIRIYVPNNVDINASSISSYLFISDD
                     VDSNNITYKTSSGGFSRAITENKVNRLENLNLISNSNLHLSTKSILGVKNVNIESESL
                     DISSKDEGVFINNIEEYIPENVNIKEKIGRHSNYDNEFNLYSNMPIAKYLDIEVPNSK
                     VRLDIPVNKYKFNCDIKSKEVIEEFNNDEEYDNDSYDYEHSEEHSNKYRNTREIKGLL
                     NKNLSNLEKEYTIKINSNRLEL"
     gene            2971..3507
                     /locus_tag="L3M60_003041"
     CDS             2971..3507
                     /locus_tag="L3M60_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227675.1"
                     /translation="MNKIAVSFLIIATTLLSTACMDYSISAVELVDSKESAVVKKDDD
                     VREETTSKMINSKNTTKIPIEIISKDGKIVKYLQIDEESTLKDKLRLILDTLSKEYFN
                     GLDMEVEVKEKDNLVKINLIEPDEKSRVSWKDDYLNEQNIIYTINNIIKNVIQEEDNS
                     IWIEEVEIYYNGKLIELR"
     gene            3627..4334
                     /locus_tag="L3M60_003042"
     CDS             3627..4334
                     /locus_tag="L3M60_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic protection ABC transporter
                     ATP-binding protein"
                     /protein_id="HBN6227676.1"
                     /translation="MSNYILEVKGVSKKFKDEVVVDNISIAIKHNSIYGLLGPNGAGK
                     STLLKMITGILNPTSGKIMFEGHRWTRKDLSNIGSLIESPAIYENLTARENLKVHTTL
                     LGLPDSRIEEVLKIVDLRDTFKKRSGQFSLGMKQRLGIAIALLNHPKLLILDEPTNGL
                     DPIGIEELRELIRSFPKQGITVILSSHILSEVEQVVDEIGIISNGVLGYQGEVSKEQN
                     LEELFMKVVAENRKRGN"
     gene            4336..5055
                     /locus_tag="L3M60_003043"
     CDS             4336..5055
                     /locus_tag="L3M60_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutE/EpiE
                     family permease subunit"
                     /protein_id="HBN6227677.1"
                     /translation="MEKLVRAEFQKMKRTFGIRLIWIAPIFSIIITFLLGGGQNGVYN
                     WWYTMFLPGVLTLISSLIVQKDEKKKYRGILSLPIEKKDVWTGKILYCIICCFITSII
                     FMIGIFLVGMLYKEVISVSQNIIGTILLVLLFMWQIPLCMFLASKFGMFITIIINMFF
                     TIIGVAVFSTTEMWYAFPYSITPRLMAPVLHILPNGLPVPEGSEFLNSNVILPGVLIS
                     IVLFILLSLATNAWFNNREVK"
     gene            5057..5827
                     /locus_tag="L3M60_003044"
     CDS             5057..5827
                     /locus_tag="L3M60_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic immunity ABC transporter MutG family
                     permease subunit"
                     /protein_id="HBN6227678.1"
                     /translation="MTQLVRIFCSDLIKLKRTFIILMHFCIALIGMGLCLGYYKYSSA
                     DDISKIAAYLQVIAIAFPLLSSIMCSLCIEQEYYSGSYKHMLTSSNPKYLTLISKYII
                     LICLGFGATLVSVLGFKFGVSSISNEVYFTLDFYMISIMILVGSNLFVYILHLFLSLR
                     FGKGASIGVGIVETLLSAVLLTGLGARIWPYIPCVWGVRFISIWSSFSSSKTIEYIKV
                     ESIKGYQSIGLVCGFVTILAFIILCIWFSKWEGKKSEE"
     gene            5961..7352
                     /locus_tag="L3M60_003045"
     CDS             5961..7352
                     /locus_tag="L3M60_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6227679.1"
                     /translation="MLVGKKFNNKKIVTLRTVFVRYSSLFFAITVVLVSILILSFPIL
                     LSLNIILPANYVEKQIYENKDKIISSKQVTKDLIPDLCEYGVYTLNGKVISGTFNKNE
                     SKEVWDLMRGFERRTITSSKNYIKLSRKNEVCIIRYSIVAEFVSPTLRTYLPKPELLG
                     MIIFSIIFLIEIIILSKLFGKKLNAEMELLKNTTEKIEQQDLDFVIESSKIREINNVL
                     FSMDKMKLALKDSLEKQWMLEENRKEQISALAHDIKTPLTIIHGNTDLLIEINENPEL
                     SEYMEYIAKGATQIEKYINTLIEISKTETGYILNKEVINVSEFIEDILIQIEALARTQ
                     NLNVEFSKQGNLPESITIDKELLFRAIMNVISNAIDYSPSQSKLYISVSVSNKYLKFV
                     ITDCGSGFSKADLSKATGQFYTGDLSRNSKSHYGMGLYIVNNIVQKHNGILHIENSIK
                     TGGAMVTIEIPIV"
     gene            complement(7428..>7939)
                     /locus_tag="L3M60_003046"
     CDS             complement(7428..>7939)
                     /locus_tag="L3M60_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBN6227680.1"
                     /translation="DPVMGDNGVMYPIFTEEMRQEIKELVKHSDLTTPNLTEACFLTG
                     NDYTKSDYNRDELIYIAKSVSDLGPSKVVITGILEDENILNLAYDRDNDHVFFTSVKY
                     NNCSYSGTGDIFTSILCGMLVNKHDLGVAVNTATDFIYKTINYTSQFDTDRNDGVMFE
                     NFLSDLTNI"
BASE COUNT         3111 a          825 c         1300 g         2703 t
ORIGIN      
        1 ttaaagtaat aatagtaatt atataatcca ggagtatatt ttgtattgtt ttcataaagt
       61 tttaccatag gcttattcat catgggaata cttgcattta taaagttttg tataacaaca
      121 ttatagttac tgtatttttc aataatagat tccttgactg cttttgcagc acttatatgt
      181 ccagctccaa attgagcagt taagattaat attgttttat catctttcat tttagccaac
      241 ttatcaccac tttcataaaa tatatctgat tagagtatta tacatttata gaaggattta
      301 atcaacaata atatttaaaa ataactattg tgcagaacat agtactgtgc tattatgggg
      361 atataagaag ttttaacata gtaccttgta aaacacaata ctgtgtattt aaagaaagga
      421 tggagtatat gaacacacaa tttagaaagg gtgtcttaga aatttgtgta cttgctctca
      481 tatctaagaa agatatgtat ggatatgaga tagtacataa catttctaaa gtaatagatg
      541 taaatgaagg aacaatatat cctattctaa gaagacttac aaaagatttg tatttcgaaa
      601 catatatact agaatctaat gaaggtcctg ctagaaaata ctatagaata acttcactag
      661 gaaaagaaaa tttatccaat ctgatggaag agtggagaga atttgtaaag gctgttgaaa
      721 ttttaataag tgaagataag ggaggtaatg agcttgaata aaactgagtt tcttgaaatt
      781 ttaaaggatt atctaaaaaa agatttttct gagagcgaag ttaatgatat attgagagat
      841 tatgaagaat attttgtaga tggtcttata gaaggaaaga gtgatatgga gataatatca
      901 tcacttggtt caccaaaatc tatagctaaa gaacttatcc tacagaccaa agaaaaaaat
      961 attcaagagg aaaaaataat aaatacagat gttttaaatg ataagtttag taaatttaaa
     1021 tcagatatga aaaagggtta ttctatctta aaagatgaag ctaaagaaaa gtatagtaaa
     1081 tcaaaagaaa atatagatgg aaaaataatg agtacagata tattaaatgg taaatttagt
     1141 aggtttaaat caaatataaa aaaggattac tctatcttga aagatgaagc taaggaaaag
     1201 tacaataaat caaaagaaaa tataaatgat aaacttacac caagtataga agaaggtgta
     1261 gggggattat ctagacaagt tataaaggga ttattagtag tgttatcttt atttctaatt
     1321 gttccagcat ttgcgtttgt atgtgctatt gtatctgtta ctattgcatt agtatgttca
     1381 ttaattgctt ttgttatatt aattccattt gtgattaaat ttatagcaat ggttccagat
     1441 gttatgatgt tttgtatttt cgcaaccata gcatttgtag ggattgagat acttgcatgg
     1501 caacttttac tatttgccat aaaactaggt aaacaattac taaataatta tatacattgg
     1561 ataaagagaa aaaatattta tataaaggca aatgaaaaga tggaaaaaag aaaaaaagct
     1621 actttaaatg atgaagattt gaataaagac attaatgaag attcatataa taatgattac
     1681 tacaatagag aagaagaaag cataaaagaa actgatgaat ataatgatga tagtataaat
     1741 tttgaggcga tatataagga ccttaaagat atagaaaata agaaaggaga agataaatat
     1801 gaataagaaa cttgccatat ttgctgcggt attattggta ataggtataa tagggacaac
     1861 atggtcaggg attttagtta tgccaagctt aataaacttt ggtttagaaa aggaagctga
     1921 attaaacaaa gaaaacaaac tttatcaaga aaaagtaaat attgacaagt tagatgtatc
     1981 tgttgacaat ataaatgtta tgataagaaa aagttcaaat gaagatgtgc gtgtgacaac
     2041 aagagggaat aatgaatttt acaaatacag tgtaacccta caagataaga ctttagttgt
     2101 aaaaggcgaa agaaaatctg aaaataaaat taaaaagatt agaagtttcg accacttatt
     2161 aaatacatct ataaatagta tgttttcacg tgattataga gaaattagaa tatatgtacc
     2221 aaataatgta gatataaatg catcaagtat aagttcgtac ttatttatat ctgatgatgt
     2281 agattcaaat aatataacat ataaaacttc ttctggaggt tttagcagag ctataacaga
     2341 aaataaagta aatagattag aaaacttaaa cttaatatct aatagcaatc ttcatctatc
     2401 aacaaagtca attttaggtg ttaaaaatgt aaatatagaa tcagagtcat tggatatatc
     2461 ttcaaaggat gaaggtgtat ttatcaataa tatagaagaa tatatacctg aaaatgtaaa
     2521 cataaaggag aaaataggta gacactcaaa ttatgataat gaatttaact tatattctaa
     2581 tatgccaatt gctaagtatc tggatataga agttccaaat tctaaggtaa gacttgatat
     2641 accagtaaat aagtataaat ttaattgtga tataaagtca aaagaagtaa tcgaagaatt
     2701 taataatgat gaagaatatg acaatgattc atatgattat gaacatagtg aagaacatag
     2761 caacaagtat agaaatacga gggaaataaa agggcttttg aataaaaatc tgtcgaattt
     2821 agagaaagaa tatactataa aaataaattc aaatagatta gaattgtaaa taatttacta
     2881 taataattta taaattgatt taaaagtata aaaatataat ctatactatg gtataataag
     2941 tatcgaaaga gaattaggag agggatactt atgaataaaa tagcagtaag ttttttaata
     3001 attgctacta ctttattgag tacagcatgt atggattata gcatcagcgc agttgagcta
     3061 gttgattcta aagagtcagc agttgtaaag aaggatgatg atgtgagaga agaaacaact
     3121 agtaaaatga ttaattctaa gaatactact aaaataccca tagagataat ttcaaaggat
     3181 ggtaagatag taaagtactt acagattgat gaagaaagta cacttaaaga taaactacga
     3241 ttaatcttag acacattatc aaaggagtat tttaatggtt tagacatgga agtggaagtt
     3301 aaggaaaaag ataatcttgt taaaataaac ttgatagaac cagatgaaaa aagtagagtt
     3361 tcttggaaag atgattattt gaatgagcaa aatataatat acactataaa taatattatt
     3421 aaaaatgtca tccaagaaga agataatagt atttggattg aagaagtaga aatatactac
     3481 aatgggaaat taatagagtt aagataaaaa aagatgtgtt ttttaacaca tctttttttg
     3541 tgatttttta attcttataa aatccttata tttatgattt agaatgattc ttgtaaataa
     3601 gtttgtcaaa agaaaggatg aaaattatga gtaattatat tttggaggta aaaggagttt
     3661 ctaaaaagtt taaggatgag gttgtagttg ataatatttc aatagcaatt aaacataatt
     3721 ctatttatgg attactagga ccaaacggag ctggtaaatc cactttatta aaaatgataa
     3781 ctggaatttt aaatccaact tcaggaaaaa taatgtttga ggggcataga tggactagaa
     3841 aggatttatc aaatattggt tctcttattg agtctcctgc tatatatgag aatttaacag
     3901 caagagaaaa tttaaaagtt catactactt tacttggtct tcctgattct cgcattgaag
     3961 aagttttaaa gattgtcgac ctaagagata cttttaaaaa gcgttcaggt caattttctc
     4021 taggaatgaa gcaacgtctt ggtattgcta ttgcattatt aaatcaccca aagcttttaa
     4081 tcttagatga acccacaaat ggtcttgacc ctataggtat tgaagaacta agagaattga
     4141 ttcgttcttt cccaaagcaa gggattacag ttattttatc aagccatatc ttgtctgagg
     4201 ttgaacaagt tgtagatgaa attggaataa tttctaatgg agtactagga tatcaaggag
     4261 aagtatcaaa agagcaaaat ttagaagaac tttttatgaa agttgtagct gaaaatagaa
     4321 agagaggaaa ttaatatgga aaaattagtt agagcagaat ttcaaaaaat gaaaagaact
     4381 tttggaatta gattgatatg gatagcccca atattttcta taatcattac atttttactt
     4441 ggtggaggac aaaatggtgt ttataattgg tggtacacta tgtttcttcc aggtgtatta
     4501 acattgattt cttctctaat agtacaaaaa gatgaaaaaa agaagtatcg tggaatttta
     4561 tcattgccaa ttgaaaaaaa agatgtttgg actggaaaaa tactttattg cattatatgt
     4621 tgttttataa cctcaattat ttttatgata ggtatatttc ttgttgggat gttatataag
     4681 gaagttattt cagtaagtca aaatattatt ggaaccatat tattagtttt attatttatg
     4741 tggcagattc ctctttgtat gtttttggca tcaaaatttg gtatgtttat tacaattata
     4801 ataaacatgt tttttactat aattggagtt gccgtttttt cgactacaga aatgtggtat
     4861 gcatttccat attcaattac tccacgatta atggctcctg tattgcatat tctaccaaat
     4921 ggacttcctg taccagaagg aagtgagttt ttaaatagta atgtaatttt gccaggagtg
     4981 ttaatatcta ttgttttatt tatattactt tctttagcta caaatgcttg gttcaataat
     5041 agagaggtga agtaatatga cacaactagt aagaattttt tgttctgatt taataaaatt
     5101 aaagcgaact tttataatac taatgcactt ttgcattgct ttaataggca tgggattgtg
     5161 cttaggatat tataagtatt caagtgctga tgatatatca aagatagctg catatttaca
     5221 agttatagca atagcttttc ctttattatc aagtattatg tgttcattat gtattgaaca
     5281 agagtattat tcaggaagtt ataaacatat gttaacatca tctaatccaa aatatcttac
     5341 acttataagt aagtatataa tattaatctg ccttggtttt ggagcaacac tagtatctgt
     5401 attaggtttt aaatttggtg tatctagtat ttctaatgag gtttatttta cattagattt
     5461 ttatatgatt tcaattatga ttttagttgg gagtaattta tttgtatata tattacattt
     5521 attcttaagt ttaagatttg gaaaaggagc ctcaattgga gtgggtatag tagaaacttt
     5581 actctcagca gtgttgctaa ctggattagg tgctagaatt tggccttata ttccttgtgt
     5641 atggggtgtt agatttataa gtatatggtc atctttttca agttctaaga caattgaata
     5701 tattaaagtc gaatcaatta aaggatatca atcaatagga ttagtatgtg gatttgtaac
     5761 cattttagca tttataattt tatgtatttg gttttctaaa tgggagggta aaaagtctga
     5821 agaataaaaa taaaaaagtt actatttact tatattatta tgttagaata tagtttagtg
     5881 ataaaaataa aaaataatca aatcaaaagc tagtttataa ttaactataa actaattaaa
     5941 gtttggagga tgtggtgagg atacttgtag gtaaaaagtt taataataaa aagattgtaa
     6001 cacttagaac tgtttttgta agatattcat ctttattctt tgctataacc gttgtcttag
     6061 tatctatatt gatattatca tttcctatat tattatcatt aaatataata ttacctgcta
     6121 attatgtgga aaaacaaatc tatgaaaata aagataagat tatttcaagt aaacaagtaa
     6181 ctaaagattt aattccagat ttatgtgagt atggagtgta tactttaaat ggcaaagtaa
     6241 tttcaggaac tttcaataag aatgagtcaa aagaagtatg ggatttgatg cgaggatttg
     6301 aaagaaggac tattacgtca tcaaaaaact atatcaaatt gtccagaaaa aatgaagttt
     6361 gtataatacg ttatagtata gtagcagaat ttgtttctcc aactttaagg acatatttac
     6421 caaagccaga actattagga atgattattt ttagtataat atttcttatt gaaattatta
     6481 tactatcaaa attatttggc aaaaaattaa atgctgaaat ggaactttta aaaaatacta
     6541 cagaaaaaat agaacagcag gatttagatt ttgttataga atctagtaaa atccgtgaaa
     6601 tcaataatgt tcttttttct atggataaaa tgaagttagc tttaaaagat tctttagaaa
     6661 aacagtggat gttagaagag aatcgtaagg aacagatatc tgcattagca catgatataa
     6721 aaacaccatt gactattata catggaaata ctgacttact aattgaaata aatgaaaatc
     6781 cagagttatc agaatacatg gaatatatag caaaaggtgc cacccaaata gaaaaatata
     6841 ttaatacact aatagagata tcaaaaacag agactggtta tatattaaat aaagaagtaa
     6901 ttaatgtaag tgaatttata gaagatatct tgattcaaat agaagcatta gctcgtacac
     6961 aaaacttgaa tgttgaattt tctaaacaag gtaatttgcc tgaaagtata actatagata
     7021 aagaattgtt atttagagct attatgaatg taatttcaaa tgctatagat tattcaccaa
     7081 gccagagtaa actatacata agtgtatcag taagtaataa atacttaaaa tttgtgatta
     7141 cagattgtgg aagtggtttt tctaaagcag atttgtcaaa ggctacagga caattttata
     7201 caggagattt aagtagaaac tctaaatctc attatggaat gggtctttat attgtaaata
     7261 atattgtaca aaaacataat gggattttac atattgaaaa ctctataaaa actggtggtg
     7321 ctatggtaac aattgaaata ccaatagttt agtaaataat atccattttg taaataccta
     7381 aattttatga ttcataggta tttataaaat ggataatttt tttgatttta tatattagtt
     7441 aaatctgata aaaagttttc aaacataact ccatcatttc tatcagtatc aaattgagat
     7501 gtgtaattta tagttttata tataaaatct gttgcagtat taacagctac tcctaaatca
     7561 tgtttattta ctaacatacc acaaagtata gatgtgaata tatctccagt tccgctataa
     7621 gaacaattat tgtatttaac tgatgtaaag aatacatggt cattatctct atcataagct
     7681 aagtttagta tgttttcatc ttctaatata ccagtaataa cgactttgct aggtcctaaa
     7741 tcagatacag attttgcaat atatataagt tcatctctat tataatcact tttagtataa
     7801 tcatttccag ttaagaaaca agcttctgtt aaattaggag tagtcaaatc tgaatgttta
     7861 actaattctt taatttcttg tctcatctct tctgtgaata ttggatacat aactccatta
     7921 tcacccatca cagggtcaa
//
LOCUS       DAFNNB010000157         7933 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0162, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000157 DAFNNB010000000
VERSION     DAFNNB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0162"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(46..516)
                     /locus_tag="L3M60_003047"
     CDS             complement(46..516)
                     /locus_tag="L3M60_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/dioxygenase
                     family protein"
                     /protein_id="HBN6227681.1"
                     /translation="MKYQVSLLAVKDIEVSKKFYQELFEQEIELDLGKNVTFRGGFAI
                     QEDFAWLTGINPDTIIQKSNNMELYFEVDNFDKFIEKLNSYKEVEFVHKPLKYEWQQR
                     VVRIYDPDKHIIEIGESMEVIARRYLAEGYSAEETSKIIQHPIEFVKQCENSKQ"
     gene            complement(1010..1399)
                     /locus_tag="L3M60_003048"
     CDS             complement(1010..1399)
                     /locus_tag="L3M60_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6227682.1"
                     /translation="MTIKKLPQSELKIMKFIWKSDSKVTSRDIVLGMEQKYKWKQTTT
                     LTLLSRLVVKRFLNSQKIDKYTHYEVLIKEKEYIGVETRDFFRNIHDSSIKSLLLSLH
                     ENINLSKDDILLIEEWIKNLKEEEEDV"
     gene            complement(2482..3888)
                     /locus_tag="L3M60_003049"
     CDS             complement(2482..3888)
                     /locus_tag="L3M60_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6227683.1"
                     /translation="MDSVKYILKRFIGSSILISLLFIILNIAGFLLFSYMIFVSKYSV
                     DLDTSDSREIKSNLIMLEENLILKDGKFILNDSSKEVLHKNKIWAMLINNNGDRVWGF
                     DLPKEIPSHYTLTDVAKFSRFFLKDYPVYVWEYPNGILVTGYPKDKYTKFNLTYTIKE
                     IKQFPMLIICMFLVNAIVIFLIALFIGLKMVKSIKPIINGIKLISNGEPVILKEKGIL
                     SDISKSINTVSKELQMKDEKLRNKDEARSNWIAGISHDIRTPLSMIIGYAGELEESRN
                     LSNKEQEQVSIICNQGVKIRELVNDLNLVSKLEYNMQVLNCDKIRVSTFLRELVSEFI
                     NDNLDNDFYIELDILNEDIIVDADKKLLKRAISNLIQNSITHNQQGCNIKVISKSDLD
                     FCYIIVEDDGKGMSQEKINSLLKSQNGLSEAYAKGQNHGLGILIVAGIINAHGGKLDI
                     TSIDGKGLKTILKLPIIK"
     gene            complement(3933..4640)
                     /locus_tag="L3M60_003050"
     CDS             complement(3933..4640)
                     /locus_tag="L3M60_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227684.1"
                     /translation="MESIKDKKILVVDDHKELLKMIDEILRKEGFSRIFLASSCEEAV
                     RVFRNIKPDCAILDVVLPDGDGFSIMRKIRETSKMPVIFLSARGEDEDRLIGLGLGAD
                     DYIVKPFLPKELTLRLVSILNRVYVPIEEEELPVFKLGDSVVVNLNSACVEKDGQEIS
                     LTAKEHSLLLKLYENKGRIVTSDSLCQAIWGDDMYGYENTLMVHIRRVREKIERTPST
                     PKYLLTVRGLGYKLMIN"
     gene            4801..5724
                     /locus_tag="L3M60_003051"
     CDS             4801..5724
                     /locus_tag="L3M60_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6227685.1"
                     /translation="MKHMEMIIRTQRLSKEYNKIFRVKDIDLRVPKGAVYGFLGPNGA
                     GKSTTLKMLLGLAKPTQGNINILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENM
                     IIMQRLLDLPKKNINEALKIVRLENQKNKKVSQYSLGMKQRLGIAMAIMKLPKLLILD
                     EPTNGLDPAGIEEIRELIKSLPEKYGMTVLISSHLLSEIDQIATSIGIINHGELVFQD
                     IIKELHNRGQSQIAIKTNNIVSAQKLLNTKGYMSLIQDGYLTIENLKDSDVAKINNML
                     VHSNIDVFRIEEHKKSLEDIFLELTGKAVTL"
     gene            5724..6485
                     /locus_tag="L3M60_003052"
     CDS             5724..6485
                     /locus_tag="L3M60_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227686.1"
                     /translation="MKYLGLEFYKLKRKKIILMTFLFVAVEIMWCIINSNRAISRNPD
                     MLGGFEYSYMLMSFASLNGLFFPILISIITSRISDIEHKGDTWKLLKSSVASLNGIYL
                     SKFLCSAILVSVPVLIQVLSIVLFGYFRGVIESLSVFLLLKYILGTVFVNIAIIALHL
                     WIAIVIPNQMVAITFGMIGSFIGLTSGLFYQGIRRLFIWSYYLELSPLSYAYDNVLGS
                     SVYRIDTNFSITILVFLIGILIYYIGRNHLLKKEV"
     gene            6497..7234
                     /locus_tag="L3M60_003053"
     CDS             6497..7234
                     /locus_tag="L3M60_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227687.1"
                     /translation="MLRKSVFGEILKLKNSFIWYVLLALPLISVLIGSGNFYLNQGIL
                     KKEWYSLWTQVSLFYGEFFFPILIAIFCAYICRLEHMNHNWNNVMTLPVNLKNIFLSK
                     LTVVSMLTAITQVFFVVFYIVAGNMFGFSGNIPKELTGWIAKGWIISISIASIQLYMS
                     IKIRSFATPIGISLCLSLFGMGLIVANPAVGIFFPYSMLGSAMGVIKQSALNVNDTIV
                     AVCVSFITTIVFSEIANRNFRKNDVIS"
     gene            7714..>7933
                     /locus_tag="L3M60_003054"
     CDS             7714..>7933
                     /locus_tag="L3M60_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227688.1"
                     /translation="MEYSKGFKVIYSLVEYSKRFKAIYSVMEYSKGFKAIYDVMEYSK
                     GFKAIYDVVKYIQGDLNQSIVLLNIQRDL"
BASE COUNT         2739 a         1187 c          917 g         3090 t
ORIGIN      
        1 gttccactac aattaatttc tactaaataa ttataattta aacaatcatt gcttactatt
       61 ttcacactgc tttacaaatt caattggatg ttgaattatt ttagaagttt cttctgcaga
      121 gtatccctca gctaaatacc ttctagctat aacttccata gactctccta tctcaataat
      181 atgtttatct gggtcataaa tacgcactac cctttgttgc cattcatatt ttagtggctt
      241 atgaacaaac tctacttctt tgtaagaatt gagtttttca ataaatttgt caaaattatc
      301 aacctcaaaa taaagttcca tattatttga tttttgtata attgtatctg gattaatccc
      361 tgtaagccat gcgaagtctt cttgtatggc aaatcctcct ctaaatgtta catttttacc
      421 taagtccaac tcaatctctt gctcaaataa ttcttggtaa aatttttttg atacttcgat
      481 atccttcact gctaataatg aaacctgata tttcataaat tttcctccta attgtttatt
      541 tgttctttat ttctattttt ttcaatatct tctattaaca cttttaccta tgtaacctca
      601 atcccttcta ttaatccttc atttttactt ttcagtatct tttattaagc ccttattttt
      661 actttttcaa catcttatat taacacttca cttctgtctc ttcaatatct tcattaatag
      721 ctatttattt tatcatctta agcttttaca gtttattttt aaacttctat ttaaatctca
      781 ttgtcttcat taaaaaagct acctagtact tatataatta taggtagctt ttttaaattt
      841 aattaaatag aatatccatt actaatttat gtttacattt aacctgtttt agattcacat
      901 tataatgtta taactcactt ataataagtt attatattac ttaagtagac taattatata
      961 aatatatcta cacacgattt atattgatgc tatacaaaaa tacgtttgcc tatacatctt
     1021 cctcctcttc ttttagattt tttatccact cttcaataag taagatatca tctttgctca
     1081 gattaatatt ttcatgtaat gataataata aactttttat agaagaatca tgaatatttc
     1141 taaaaaaatc tctagtttca actcctatgt attccttttc tttaattaaa acttcataat
     1201 gtgtatattt atctatcttt tgagaattta gaaatctttt tactactaat ctagataaaa
     1261 gagtcaaagt tgtcgtttgt ttccatttat atttttgctc cattcctaaa acaatatctc
     1321 tagatgttac tttggaatct gatttccata taaatttcat tatctttaat tctgattgtg
     1381 gcaacttttt tatagtcatg ataaaacctt gctactcaga atatagacaa gcaatatcca
     1441 taataaaatt cttcgattaa atacatctta tacggattta tataagctca gatttataag
     1501 atatttttca atcttaatcc taaatttagc tatttgtaca tggtaaatat atataaataa
     1561 tgtcaaaatt ttcgtattat attgcttaat tttatgaaat attttgtact cagctatcta
     1621 ttttggatac ttgtccagta gctctccttt cttttaaatt atttgatttt aaatatttgt
     1681 attccagtga atcctacttc ctaaatttct tacttaatac aacttattac taaacttcct
     1741 acttcctaaa tttcttactt cttaagaata gtgttaattg taattttaag attataattt
     1801 ttgccttgtt taattgaaac attctatata gttatatcct tctattttag agttttttta
     1861 tttatatctt acaaagttta aattttttca ttaattatat tataaatatt ctatatattt
     1921 tcatttaaat atatatatat atatatgtta tttctaaaaa tactacacaa aactctactt
     1981 tagtagaact taaagacgca aaatacttca atttaaaaca tttatcgtac tttaaataaa
     2041 taactataat aaaatataca tacattagtt acataaaagc tattataact acacgtaaca
     2101 gcgtacaatt aaaacttttt tagcatgtca ttatattttt tcttaaaatt aaaaaacact
     2161 atatcataat tctattttag tagaattttt ttgtcaataa taaaattaaa tattaattaa
     2221 aaaataaaaa aaatggaatt atctaaagaa tttaataacc ttacaataat tccaaaatat
     2281 tatttctatg aaaattttga taccatgagt aacttagcta taagcgcata aagcacaagt
     2341 tactctttag tttaataaaa cttgacaaga aaagaattta tccttattct actctaatag
     2401 agttactttg tcaataaaga ataaaattat tttcaaaata tttccattct tcatttttat
     2461 ctgtcattat tttataaaat atcacttaat tataggtaat tttagtattg tttttagtcc
     2521 cttaccatca atactcgtaa tatccaattt gcctccatgt gcattaataa taccagccac
     2581 tatcaaaata cctagaccat gattttgacc tttagcatat gcctcactta gtccattttg
     2641 actctttaat aaactattaa ttttttcttg actcatacct tttccatcat cttcgacaat
     2701 tatatagcaa aaatctaaat cagacttaga tataaccttt atgttacacc cttgttggtt
     2761 gtgagttata ctattttgta ttaaattact aatagccctt tttaataatt ttttatctgc
     2821 atctacaata atatcttcat ttagtatatc aagttcaata taaaagtcat tatccagatt
     2881 atcatttata aactcagaaa caagttctct caaaaacgta gaaactctaa tcttatcaca
     2941 attcaatact tgcatattat attcaagttt agacactaaa ttgaggtcat ttactaattc
     3001 tctaatttta accccttgat tgcatataat agacacttgc tcttgctctt tatttgaaag
     3061 attacggctt tcttcaagtt ctccagcata tccaattatc attgaaagtg gtgttcttat
     3121 atcatgagat attcctgcaa tccagttgga cctagcttca tctttgttcc taagcttttc
     3181 atctttcatt tgcaattctt tagatacagt attaatagat tttgaaatgt cactcaatat
     3241 tcccttttct ttgagaatta ctggttcgcc atttgatata agctttatcc cattaataat
     3301 tggttttata gatttcacca tttttaagcc tatgaataga gctattaaaa aaataactat
     3361 cgcattaacc agaaacatac atattattaa cataggaaat tgctttattt ccttaatcgt
     3421 ataagtaagg ttaaacttag tgtacttgtc ctttggataa cctgttacta atattccatt
     3481 tggatattcc catacataca caggataatc ctttaaaaag aaccttgaaa actttgctac
     3541 atctgtcaaa gtataatgac ttggaatttc ttttggtaaa tcaaatcccc atactctatc
     3601 tccattgtta ttaattaaca ttgcccaaat tttattttta tgtagtactt ccttactaga
     3661 atcgtttaaa ataaactttc catcttttaa aatcaaattt tcttcaagca taatcaagtt
     3721 ggattttatt tccctactat cacttgtatc taaatcaaca gagtatttgg atacaaatat
     3781 catatatgaa aacagaagaa aacctgctat atttaatata ataaaaagta atgaaatcag
     3841 gatacttgaa cctataaatc gcttcaatat atactttaca ctatccaaaa ttaaatcact
     3901 cctcttgatt tcttttatcc taatagctaa aattaattaa tcatcaactt atatccaaga
     3961 cctcttactg taagaagata tttaggtgta gatggagttc tttctatctt ctctctaacc
     4021 cttctaatat gaaccataag ggtattttca tatccataca tatcatcccc ccaaatagct
     4081 tgacatagtg aatcactcgt aacaattcta cctttatttt catacaactt taaaagcagt
     4141 gaatgctctt tagctgttag agaaatctct tgcccatctt tctcaacaca tgcactattt
     4201 aaatttacaa caacagaatc tcccagttta aatactggta actcttcttc ttctataggc
     4261 acatatacac gatttaatat gctcacaagt cttaaagtaa gttcctttgg taaaaatggc
     4321 tttactatat aatcatcagc tcctaagccc aaaccaatca atctatcctc atcttcgcct
     4381 cttgcagaaa gaaaaataac tggcatttta gatgtctctc taatttttct cataatagag
     4441 aaaccatctc catctggcaa tacaacatct aaaattgcac aatctggctt tatatttcta
     4501 aataccctaa cagcttcttc acaacttgat gctaaaaata ttctactaaa tccctctttt
     4561 cttaggattt catctatcat ttttaataat tccttgtggt catcaaccac aagtattttt
     4621 ttatctttta ttgactccac aatcatcacc ctcctttagt ataccatata ccattttcac
     4681 agctttgcct aaatagacta tttaaggcta ttgtaaggta aacttcagct aattgaaatg
     4741 tagttcattt attatactta tataagctaa aaaaatcaat aaacacagat taaaaagggg
     4801 atgaaacata tggaaatgat aattagaaca caaagacttt caaaagagta caataaaata
     4861 tttagagtaa aggatataga cctaagagtt ccaaaaggag ctgtatacgg ttttttaggt
     4921 cctaatggag ccggaaaatc taccactctt aaaatgcttc ttggacttgc taaacccaca
     4981 caaggaaata ttaatatttt aggtaaagaa ttaaatgaaa aaaatagaat atcaatttta
     5041 aaagatatag gttctttaat agaatctcca tcatactatg gtcacctgac agggcttgaa
     5101 aacatgatta taatgcaaag actcttagat ttgcctaaaa aaaatataaa tgaagcatta
     5161 aaaattgtaa ggttagaaaa tcaaaaaaat aaaaaagtta gtcaatattc tttaggtatg
     5221 aagcaacgtc ttggcattgc catggctata atgaaacttc ctaagttatt aatacttgat
     5281 gaacctacaa atggacttga cccagcaggt attgaggaaa ttagagaatt aataaaatct
     5341 ctaccagaaa aatacggtat gacagttcta atctctagcc atcttttaag tgaaattgac
     5401 caaattgcca catcaatagg aataataaat catggcgaac tggtttttca agatatcata
     5461 aaagaacttc ataatagagg tcaaagtcag attgctatca aaactaataa tattgtatct
     5521 gcacaaaagc tattgaatac taaaggatat atgtcactta tacaagatgg atatttaact
     5581 attgaaaatt taaaagactc agatgttgct aaaataaaca atatgcttgt ccattcaaat
     5641 atagatgtat tcagaataga agaacataaa aagagcttgg aagatatctt cctagaactt
     5701 actggtaagg cggtgacttt ataatgaaat accttggtct agaattttat aaacttaaaa
     5761 gaaagaagat tattctgatg acctttttat ttgtagctgt tgaaataatg tggtgcatca
     5821 taaattcaaa tagagctatt tcacgtaatc cagatatgtt aggtggattt gaatattcat
     5881 atatgcttat gagttttgca agtttaaatg gtctattctt tcctatctta atatcaataa
     5941 ttacatcacg tataagcgat atagaacata aaggagatac atggaagctt ttaaaatctt
     6001 ctgtggcatc cttaaatggt atttatttat ctaaattttt atgctctgct attttggtat
     6061 ctgtacctgt attgatacaa gtgttgtcta ttgtgctatt tggatatttt agaggagtaa
     6121 tcgagtcact ttctgtcttt ttacttttaa aatatatatt aggaacagtt tttgtaaata
     6181 ttgctatcat tgcactgcac ctatggattg caattgtaat tccaaatcaa atggttgcaa
     6241 ttacatttgg tatgattggc agttttatag gtttgacatc aggattgttt tatcaaggta
     6301 ttcgtagatt gtttatatgg tcttattacc tagagttatc acctttaagt tacgcttatg
     6361 ataatgtttt aggttcatct gtttatagaa ttgatacgaa tttttctatt acgattttag
     6421 ttttcttaat tggaatatta atttattata ttggaagaaa tcatttatta aaaaaagaag
     6481 tataggaggg atttaaatgt taaggaaatc agtttttgga gaaattctaa agttaaaaaa
     6541 ttcttttata tggtatgtat tattagcttt gccacttata agtgtattga ttggctctgg
     6601 taatttttat ctaaatcaag gcatactcaa aaaagaatgg tacagcttat ggactcaagt
     6661 tagtcttttt tatggggaat ttttctttcc tatcttaatc gcaatctttt gtgcatatat
     6721 ttgtaggctt gaacatatga atcataattg gaataatgta atgacattgc cagttaattt
     6781 aaaaaatatc tttttatcta aacttacagt agtttcaatg cttacagcta ttacacaagt
     6841 tttctttgtt gtcttttata ttgttgcagg aaatatgttt ggtttttctg gtaatatacc
     6901 taaagaattg actggttgga ttgctaaagg ttggattatt tccatttcaa tagcttctat
     6961 acagttatac atgtctataa agattagaag ttttgcaaca cctattggta ttagtctttg
     7021 cctttcttta tttggtatgg gattaatagt agcaaatcct gctgttggta tatttttccc
     7081 ttattctatg ttaggtagtg ctatgggggt tataaagcaa tctgctttaa atgttaatga
     7141 cactatagtt gcagtatgtg tttcatttat tactactata gtattttctg aaattgccaa
     7201 tagaaatttt agaaagaatg atgttatttc ttaatggtaa acgttagata tttttttgtt
     7261 taaaatttat attcttttta gttgaaatat atatcccttt tagttaaaaa tctatatctt
     7321 ttattttaat tttatatgtt ttatctatct actatataat aaatatactc ctaaaagtaa
     7381 aaaatatata ataatgttat tatttaaaat tattatatat tatattaaaa cttaatgcaa
     7441 aaacaatttt tatattatat aatgaaactt aattaaatat aactaaaaaa tcattaaata
     7501 tattaaataa atatttaact ttaatgacct atatttaatt atggctataa catagaatta
     7561 tcaaatatat tgaataaata tttgaccctt gaaatgtgta attaaattaa gtttggttat
     7621 ttgatggaat gcaaatttga tataaaaact ccatttaaag tgccagataa aaactttatg
     7681 gttatataaa agctaaaagt aatttatagt gttatggaat attcaaaggg atttaaagta
     7741 atctatagtc ttgtggaata ttcaaagaga tttaaagcaa tctatagtgt tatggaatat
     7801 tcaaagggat ttaaagcaat ctatgatgtt atggaatatt caaagggatt taaagcaatc
     7861 tatgatgttg tgaaatatat tcaaggggat ttaaatcaat ctatagtttt actaaatatt
     7921 caaagggatt tga
//
LOCUS       DAFNNB010000158         7816 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0163, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000158 DAFNNB010000000
VERSION     DAFNNB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0163"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..528
                     /locus_tag="L3M60_003055"
     CDS             <1..528
                     /locus_tag="L3M60_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6227689.1"
                     /translation="KIGILLVFYFLFSQIIFGLIISDNQFDYKNFLNYILLPIISLVY
                     IYPFLCEEYGWRSFLQSILFDRFGKKIGIVMVGTCWSLWHLPLQFTLYSPQTPIIGAI
                     AHLIYGIGLSIFLGYVYMKTKNIWFCSIMHVLINSLGLIFNDSEIIINYHSIIQRLIF
                     ILLFYTPFLFTKEYK"
     gene            908..2434
                     /locus_tag="L3M60_003056"
     CDS             908..2434
                     /locus_tag="L3M60_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN6227690.1"
                     /translation="MNESYWFLNSNPKEYNKLGENIKTDCLIVGGGITGLTTAYLLAK
                     EGKKVVLVEADKIGYGTSGRNTGKVTCQHDIFYSKIEKKYGLDKAKSYYNANNEALNL
                     VEQIIEENNIKCDFKRETSFVFTEKEDTIKNIKDEYRTCKKIGINCEYRETIENIPLD
                     IKGAISFTNQGQFNPKKYIDGLAKAAVNLGLKIYENTPVVDLEKGKICRVKTREDNII
                     EAENVIISSHSPWYDGLNFYFAKEYAERAYLMAAVLENKLADGMFISIDDPSITFRQY
                     NDGNENLLIFGGGDHKVGQGGTEKEIFDDLEHYGKKVFKVKDFKGKWSAQDNMSFDNV
                     PYIGYINKREDNIYVATGFSKWGITNGTAAGIIIKDLIINNNSDYKDTFNPSRLGSYF
                     SKDFIKENANVAINYVSGKLKIGSGDMPKNNGEGKIVNIDGKRYGVYKDDNGDFYIVD
                     TTCTHLGCELNFNSEEKTWDCPCHGSRFDYKGNILEGPALKPLKLYGHGDNDVNPKLL
                     "
     gene            2462..3019
                     /locus_tag="L3M60_003057"
     CDS             2462..3019
                     /locus_tag="L3M60_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227691.1"
                     /translation="MNKLLKPITILLYIVYILSAVFFVANKEYGSLGLVAISLVGTFI
                     LSFINKQTERLLSKELYIMLALFIMFSSLLGTCFNFYDINYYDDFLHLWSGIISCIVA
                     FSIIKYFYTQREISKMSKLFLVIFLFMFSMGVASLWEIGEFLMDTFIGTTTQAGGLED
                     TMIDMVDALIGTIITIPFIVKKSSI"
     gene            3541..4584
                     /locus_tag="L3M60_003058"
     CDS             3541..4584
                     /locus_tag="L3M60_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227692.1"
                     /translation="MLKKWFGIVKKRQKSESVKEEGEVILKNEKILSEEKSIDEEGVV
                     VNIDNEILTKVEVVNDDNEIQEKIIEEDWLISENTLKLDDKEAIINDKDIELCKKEVQ
                     VESEKIDLNKFEELDQNENYNLEKNVIEEKEVSECLTEEDLEYIKEIKIKRGKSIKAI
                     NLYTKEEWVFDTHKQCSKKLKVPLGYIRENLKYGYMDYFGDAINYLSEVLNIDEYCKN
                     EWSYLDNSKSPSEIFNILNNKIFSIRLSNEKRNEILTNDKIEALKMNYKFECIDEEYD
                     EYFKKYKSIIKRGGKKKVELVNKKGDVLEIFKSLEECAIYLQKEKNEVIQMLKYGDTK
                     VGRNFIRYSLRSI"
     gene            4695..5837
                     /locus_tag="L3M60_003059"
     CDS             4695..5837
                     /locus_tag="L3M60_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBN6227693.1"
                     /translation="MSEEKNPKENTLDESKNSDNVNSKKDDVSDEKNTDNKIKKREVK
                     LNNKTLKAIVAIVAVGAICLGGGYVFGKEAGRKLPATSKHYNSSEVVATIGDKKITGE
                     MLRKRMEPLFYLNAKTTMTDDEIRSYESNMIETMITSDILSSEAAKEKISITNDQLDA
                     QYNNLMSSIESAMGMTKEEFLKQFDLTEKYIKDDMKKELVASKYLNEKSKVTDKEAEN
                     YYNKNKNNYLQVRASHILIKTVDDKGKQVSNSKKAELKKEAEEILKKAQAGEDFATLA
                     KKYSEDSSAESGGDLGFFGKGQMVESFEKAAFALKKGEVSNKLVESDYGYHIIKKTDE
                     KYQPFEEIKSDLVSSLTSEKQSLLIQNLKEKYNVKITDKYQMKAKE"
     gene            6295..7686
                     /locus_tag="L3M60_003060"
     CDS             6295..7686
                     /locus_tag="L3M60_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBN6227694.1"
                     /translation="MSSTESVSKKGGHPKSFWLICMTILWERFSYYGLTALLVLFFTA
                     SVAQGGLGLSTQEATSMFGLFTGLIYLTPLVGGWLADKVLGQQKCISIGALLIAIGDF
                     VLFASSTSITMVWVGLSIIILGNGFFKSSCSNIVGDIYPKTDVSRKDAAYSLFYMAVN
                     VGAFIAPIICGLLSDNVFAVRGASGDIVSYGYKMAFLICGIGMMLGMIIFTLLAPKLL
                     NEVGKYPVTKGNKGEKIEYGPLTKLEKNRIIAMVILFLFVVLFWTAYYQTQSSFMIYV
                     RDNVNRTLFGFEMPVAWLTSLNAILCVALCPVLATLWVKLSHTKRGDFSIPVKMGLGM
                     IIMGVGFIVMVFATLANGPDGMVKASILFIVLTYIFTTVGELFLSPIGLAMFNKLAPA
                     KYATLSMGAWYLTSFFASLLSGKIAGFTATLGFLQIFGGIATVLIIFGLILLGIRNVL
                     TRLMAMDLLTKEE"
BASE COUNT         3146 a          703 c         1341 g         2626 t
ORIGIN      
        1 aagataggta ttttgctagt tttttacttt ttattttctc aaattatttt tggattaata
       61 ataagtgata atcaatttga ttataagaat ttcttgaatt atattcttct tcctataata
      121 tcattagtat acatttatcc ttttttatgt gaagagtatg gttggagatc ttttttacaa
      181 agtattttat ttgatagatt tggcaaaaaa ataggtattg taatggtagg cacttgttgg
      241 agtttatggc acttaccttt acaatttaca ctatatagtc ctcaaactcc aataattgga
      301 gctatagccc atttgatata tggtattgga ctttctatat ttttaggtta tgtttatatg
      361 aaaactaaga acatatggtt ttgctctata atgcatgttc taattaatag cttaggatta
      421 attttcaatg attctgaaat aattattaat taccatagta ttatacaaag attgatattt
      481 atactattat tttatactcc ttttctattt acaaaagaat ataagtaaga attacaatta
      541 atttatataa aataaatttt taagtaaata tttttataga gagattgtta tattacatat
      601 cagaaaaata ttaaacgaac taaatcaata aaaaagttgg tggctaacaa tttataattg
      661 ttagcacaac atcatcttat gtattgaaat ctaaaatatt ctcattttta cttagcataa
      721 atactattaa ttaagaaaat aatatagaaa ttataaatat atataagttt agtctggtgt
      781 ataaaacaag aaaactaagt gatttttaat ttagcttttt attaagctta ttttttatac
      841 tatttataat aaataaactc catgatggga aaactattac tatttaatat gtaaaggagt
      901 attttaaatg aacgaatcat attggttttt aaactcaaat ccaaaagaat ataacaaatt
      961 aggtgaaaat ataaaaacag attgtcttat agtaggtggt ggaataactg gattgacaac
     1021 tgcatattta ttagcaaaag aaggtaaaaa agtagtgctt gttgaggctg ataaaatagg
     1081 atatggaact tctggaagga atactggtaa ggtaacttgt cagcatgaca tattttactc
     1141 taagatagag aaaaaatatg gattagataa agcaaaatca tattataatg caaataatga
     1201 agctttaaat ttagttgaac aaataataga agaaaataat ataaaatgtg attttaaaag
     1261 ggaaacttct tttgtattta cagaaaaaga ggatactata aagaatatta aagatgaata
     1321 tcgtacatgt aaaaaaatag gaataaattg tgagtatcgt gaaactatag aaaatattcc
     1381 attagatata aagggggcta ttagttttac taatcaaggt caatttaatc caaagaagta
     1441 tattgatgga ttggcaaaag cagcagtaaa tttagggcta aaaatatatg aaaatacacc
     1501 agtagtagac cttgagaaag gcaaaatatg tagggttaaa acaagagagg ataatataat
     1561 agaagctgag aatgttataa tttcatccca tagtccatgg tatgatggat tgaattttta
     1621 ttttgcaaaa gaatatgcag agcgtgctta tttaatggct gcagttttgg aaaataaatt
     1681 agcagatggt atgtttataa gtatagatga cccatcaata acttttagac aatataatga
     1741 tggaaatgaa aatttactga tttttggagg aggagaccat aaagtcggtc agggaggaac
     1801 tgagaaagaa atatttgatg atttagagca ttatgggaaa aaagtcttta aggtaaaaga
     1861 ttttaagggg aaatggtctg ctcaagataa tatgagtttt gacaatgttc cttatattgg
     1921 ttatatcaac aaaagagaag acaatattta tgtagctaca gggttctcta aatgggggat
     1981 tactaatgga actgctgctg gaataataat aaaagatttg ataataaata ataattctga
     2041 ttataaagat acttttaatc catccagatt aggtagctac ttttcaaaag attttataaa
     2101 ggaaaatgca aacgtagcaa taaattatgt aagtggaaaa ttaaaaatag gtagtggaga
     2161 tatgcctaaa aataatggag aaggaaagat tgttaatatt gatggaaaaa gatatggtgt
     2221 atataaggat gataatggag atttttatat tgtagacacg acatgtactc atttaggatg
     2281 tgaacttaat tttaacagtg aagaaaaaac atgggactgt ccatgtcatg gttctagatt
     2341 tgactataag ggaaatatac ttgaaggacc agctcttaag ccattaaaac tttatggaca
     2401 tggtgataat gatgttaatc ctaagttatt ataaaattat ttttaggaag gtaaagtgta
     2461 tatgaataaa cttttaaagc caataactat actattgtat atagtttata ttttatctgc
     2521 tgtatttttt gtagcaaaca aagaatatgg aagtctaggt ctagtagcaa ttagtttagt
     2581 tggtactttc attttgagtt ttataaataa gcaaactgaa agattattga gtaaagaatt
     2641 atatataatg cttgctttat ttataatgtt ttcatcatta ttaggtactt gttttaattt
     2701 ttatgatata aattattatg atgatttttt gcatttatgg tcaggtataa tatcttgtat
     2761 tgtggctttt tctataatta aatactttta tacacaaaga gagataagta aaatgagtaa
     2821 attgttttta gttatatttc tatttatgtt tagtatggga gtagcgagtt tatgggaaat
     2881 aggtgaattt ttaatggaca cttttattgg gactactaca caggctggag gattagaaga
     2941 tactatgatt gatatggtag atgctttaat aggtacaatt ataactattc cttttatagt
     3001 aaaaaaatct agtatatagt tttaaatttt ttatattagt tataatggat ttaattaatc
     3061 atgttgagtt tcaaggtcta tatgattaat taaatccatt tttgttattt ataattatta
     3121 ttgaatatat ttttacatat gagatatacg gtgatagata gaaattgttg gattatgtaa
     3181 ataattaata tataatacca ttatgggcaa taaatacaaa tcaaatgata atatagtcta
     3241 tttatgtaaa tgttatattg tatcctaagt ataatagtaa gttttttgat aatggtgtag
     3301 aaagtaaaaa tctactagtc taaattgatg tatggattag tttaggaaca gtaattgcat
     3361 atcttaaatt tgatgtcatg ttgagctact agaaataggt catctttttc acttgaatat
     3421 cttttcttta aacatgggga gtatcatatc aaaaacatat gaaaattaag tgatttataa
     3481 gaacagtaat attttatatg atagtataga atagtaataa agtattagga ggaacattga
     3541 gtgttaaaaa agtggtttgg tattgtgaaa aaaagacaaa aaagtgagtc agtaaaagaa
     3601 gaaggtgaag taatattaaa aaatgaaaaa atattatctg aagaaaagtc gatagatgaa
     3661 gaaggagttg tggttaatat tgataatgaa atattaacta aagtagaagt agtaaatgat
     3721 gataatgaaa tacaagaaaa aataatagaa gaagattggt taattagtga gaatacctta
     3781 aaattagatg ataaagaagc aattattaat gataaagaca tagaattatg taaaaaagaa
     3841 gttcaagttg aaagtgaaaa aatagattta aataagtttg aagaacttga ccaaaatgaa
     3901 aattataatc tagaaaaaaa tgttattgaa gaaaaagaag taagtgaatg tttgacagaa
     3961 gaagatttag agtacataaa agaaattaaa ataaaaagag gaaaaagtat aaaagctata
     4021 aacttgtata ctaaagaaga gtgggttttt gacactcata aacagtgtag taaaaaactc
     4081 aaagttccat tagggtatat acgagaaaat ttaaaatatg gatatatgga ttactttgga
     4141 gatgcaataa attatttaag tgaagtatta aatatagatg aatactgcaa aaatgaatgg
     4201 agctatctcg ataatagcaa atctccatct gagatattta atattctaaa caataaaata
     4261 tttagcataa ggctttctaa tgaaaaaaga aatgaaattt tgacaaatga taaaattgaa
     4321 gcattaaaaa tgaattataa atttgaatgt attgatgaag aatatgatga atattttaaa
     4381 aaatataagt ctataatcaa aagaggtgga aagaaaaaag ttgaattagt aaataaaaaa
     4441 ggtgacgttt tagaaatatt taagtcttta gaagaatgtg ccatttattt gcaaaaggaa
     4501 aagaatgaag ttatacaaat gttaaaatat ggagatacaa aagtaggaag gaactttata
     4561 aggtatagtt tgagaagtat ttaatttcat tttaaattca taagagtttc atttctgatt
     4621 cacacattta atattatttt aatgtaaaat aataaaggtg aagtattgca caattaaaat
     4681 taaggagggt attaatgtct gaagaaaaaa atccaaaaga aaatacatta gatgaatcta
     4741 aaaattcaga taacgtaaat tctaaaaaag atgatgtgtc agatgaaaaa aatactgata
     4801 acaaaattaa aaaaagggaa gtaaagctaa acaacaaaac acttaaagct attgtagcaa
     4861 ttgttgcagt aggagctata tgtttaggtg gaggctatgt ttttggcaaa gaagctggaa
     4921 gaaaattgcc tgctacttca aaacactata atagcagtga ggtagtagct actattgggg
     4981 ataaaaaaat aacaggtgaa atgttaagaa agagaatgga gccattattt tatttaaatg
     5041 caaaaactac tatgactgat gatgaaatac gttcatatga atcaaatatg attgaaacta
     5101 tgattacatc tgatatatta tcctctgaag cagcaaaaga gaaaataagt ataactaatg
     5161 accaactaga tgctcagtat aataatttaa tgagtagtat agaatcagca atgggaatga
     5221 ctaaagaaga gtttttaaaa cagtttgatt tgactgaaaa atatataaaa gatgatatga
     5281 aaaaagaact ggtagcatca aaatacttaa atgaaaaatc aaaagttact gataaagaag
     5341 cagagaacta ttacaataaa aacaagaaca attatttaca agttagagct tcacatattt
     5401 taataaaaac tgttgatgat aaaggtaaac aagttagtaa tagtaaaaaa gctgaactta
     5461 agaaagaagc tgaagaaata cttaaaaaag cccaagctgg ggaagatttt gctactctag
     5521 ctaaaaaata ctctgaagat agtagtgcag aatcaggcgg agatttaggt ttctttggta
     5581 aaggtcagat ggtggaatct tttgaaaaag cagcatttgc attgaagaag ggagaagtat
     5641 ctaacaaact tgtagaaagt gattatggat atcatataat taagaaaact gatgaaaaat
     5701 atcaaccatt tgaagagata aaatcagatt tagtaagcag tttaacatca gaaaaacaaa
     5761 gcctacttat acaaaatcta aaagaaaaat ataatgttaa aatcacagat aaatatcaaa
     5821 tgaaagctaa agaatagata aagtcaattt ataaaaaaaa agaaaaattt actgtatgga
     5881 taaaagctat ttttgctctc aatatagaga gtctaaaata gcttttatta tttttgaata
     5941 aaatctattt acatatatga agtgacaaag aaataacatg caaaaaaata ttaacaagaa
     6001 atatccttaa tttgaattat tttagataaa acatttataa aaatgaaaaa ataaacaaaa
     6061 aataattgaa gatttgagct gttgtttaaa tatatatata tattaagatt ttctactgaa
     6121 aatactgaaa aaataatatt tttcataatt ttaaaaataa taataaaaaa atgaaaatac
     6181 aggcgataat ctattgagta aagatagaaa agatgatatt ataagtatgt gctaaaatgg
     6241 ttaaagcaat cttaatatta aatttaacta taaaacaaaa gggggcgttt ttttatgtca
     6301 agtacagaaa gcgtatctaa aaaaggtggt catcctaaga gtttttggct tatttgcatg
     6361 acaattttat gggaaagatt tagttattat ggtttaacag ctttattggt attatttttt
     6421 acagcaagtg tagctcaagg tggattaggt ctttctactc aagaagcaac atctatgttt
     6481 ggtttattta caggtcttat atatcttaca ccattagtcg gtggttggct agcagataaa
     6541 gttttaggtc aacaaaaatg tatttctata ggtgcacttt taatagcaat aggagatttc
     6601 gtactatttg cttcatctac atctattacc atggtttggg taggtctatc aataattatt
     6661 ttaggaaatg gtttttttaa atcaagttgt tcaaatatag ttggggatat atatcctaaa
     6721 actgatgtgt caagaaaaga tgcagcatac agtttattct atatggcagt aaatgtagga
     6781 gcatttatag ctccaataat atgtggatta ctttctgata atgtgtttgc agtaagagga
     6841 gcaagtggag atatagtatc ttatggatat aagatggcgt ttttaatttg tggtataggc
     6901 atgatgttag gtatgattat atttacactt cttgcaccaa aattattaaa tgaagtggga
     6961 aaatatcctg tgactaaagg caataaaggt gaaaaaattg agtatggacc acttactaag
     7021 ttggagaaaa atagaataat agcaatggtt attttatttt tatttgtggt cttattctgg
     7081 acagcatact atcaaacaca aagttctttt atgatttatg taagagacaa tgtaaataga
     7141 actctatttg gatttgaaat gccagtagca tggcttacat cactaaatgc tatattatgt
     7201 gtggctttat gtccagtatt agctacccta tgggttaaat tatctcatac caaaagaggt
     7261 gatttctcta ttccagttaa aatgggatta ggaatgataa taatgggtgt tgggtttata
     7321 gttatggtat ttgctacatt agctaatggt ccagatggaa tggtaaaggc tagtatattg
     7381 tttatagtat tgacttatat atttacaaca gtaggtgaat tattcttatc tcctatagga
     7441 cttgcaatgt tcaataaatt agctccagct aagtatgcta cgttatctat gggagcatgg
     7501 tatctaacaa gtttttttgc aagtttatta tcaggaaaga ttgctggttt tacagctaca
     7561 cttggttttt tacagatatt tggaggaata gcaacagttt taataatatt tggtttaata
     7621 ttattgggaa taagaaatgt attaacgaga ctaatggcta tggatttact aacaaaagaa
     7681 gaataattca tgacgaatta ttgtaaatag atgaattact ttaagtaaag gaaattacat
     7741 ttaaatataa aattaaatgt aatttcctct ttgattatga aaaaaagctt tacacaaata
     7801 gatattataa ttaaaa
//
LOCUS       DAFNNB010000159         7745 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0164, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000159 DAFNNB010000000
VERSION     DAFNNB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7745
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0164"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            87..938
                     /locus_tag="L3M60_003061"
     CDS             87..938
                     /locus_tag="L3M60_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBN6227695.1"
                     /translation="MSCILKLKQIYEDLTEVDKKIADYILNNTEAISKLSVSELASNS
                     KTSTASIVRFSRKMGYSGFGDLKIEIAKDLMGKENEYTYVDENYDCNIDSVINKITNK
                     NIETINQTRLLNEKDIIKEAVEQIIKAKNVYIFGVGGSALVALDLQMKLLRINKQAFT
                     SLDSHTQLMVSSNVDKEDISIAISYSGESKEVIKSIENAKLKGCKVICMTKYSDNHLS
                     KISDLKLVVPNIEKRLREGAISSRIAMLTLIDIIYISIIQENLNEAEQKLQETKRILD
                     YLNIDNA"
     gene            1549..2583
                     /locus_tag="L3M60_003062"
     CDS             1549..2583
                     /locus_tag="L3M60_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HBN6227696.1"
                     /translation="MKKIRKLATKLLITTMVLISGMSMTVYGMTAKEVTAKTPKSYVT
                     GTNSVYGPKLSQAQLNSVAQATADFMNKKITKNMTTDAKILVAYNHIKNNTTYVDWNA
                     VEGANTAYTLVTKKGACSGMARSMKALCDAMGIESYYVHSTSNDHQWNLIRFGDGRLY
                     HVDIDANKSAGKDVIYKSLSHPLPFDKTAYPTVGAVAQTQKKPAPPGKHTMLPHQEGA
                     KVVGKFGDIKDKHVMYEGVRCVVINSSVNTVTFMGSESNLDPSELTIAVIGFMNTPYG
                     MFALAPESKPVEYPFEYDKPFSLDFSGKNRSGAELAKYCKKNDVTIKIEITDEKANTG
                     LGQIMYIRYE"
     gene            complement(2699..3718)
                     /locus_tag="L3M60_003063"
     CDS             complement(2699..3718)
                     /locus_tag="L3M60_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227697.1"
                     /translation="MLKLSLLYLFKGGLHLNNINSLKVIKLSLGCSLAIFIAWLLKLE
                     YSMVAGVIVLLTVKDTKRETLKGSIGKVYGFLLCTIFSYLCFNILGYNLISFSVFIFI
                     IIPLCFLLNIQDVIAMCVVIASHYFLQGETSIKWILNEAGIFAVGTGIGVLINMYIPT
                     NIHKIHEVQKKLQEEVSIVLIDIADIIVNPKKENGYSRDLYTLNSLIDSSISETYDNI
                     NNTLLSDTRFFLEHMDIIKSQRDILENLYSYVSQLNSTPPQAHILSAFIHKIGHTEFE
                     AETGNLLLEELKRLMLSMKNQPLPVDRTEFENRAILFLCLTELKQFLVNRKNAQMLRD
                     NNFYK"
     gene            4145..5026
                     /locus_tag="L3M60_003064"
     CDS             4145..5026
                     /locus_tag="L3M60_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HBN6227698.1"
                     /translation="MKITEGYMPFEGFKTYYRIVGENTEGKKPLVLLHGGPGSTHNYF
                     EVLDKIAESGRQVIMYDQIGCGNSFVEGHPELFNADTWIKELIELRKHLGLDEIHLLG
                     QSWGGMQAIWYAIEYKPKGIKSYILSSTLSSARLWEKEQKRRISYMSESDQKALLDAV
                     NTGDYSSKEYNDALERFMEMYCAGEVTEDSPECLRRPKKSGSEAYIVGWGQNEFSPTG
                     TLSGYEFTDRLHEIKEPCLVTSGARDLCSPYIAKTMYDRIPNSKWELFEYSRHMPFVE
                     ENEKYIEVLTEWLDAND"
     gene            complement(6250..7035)
                     /locus_tag="L3M60_003065"
     CDS             complement(6250..7035)
                     /locus_tag="L3M60_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227699.1"
                     /translation="MKKQKIITSLLITGVLATNTYPVFASQAIQTQNLTSIEQRISSN
                     VLLKCDQSSINYTNFFKNGGGEQWVKLTLEGAKFNTNDPYEIKNAIKQNYDVQTTIAV
                     GTDGKSIEFGVYEPNAGIEYLPNWEININSSVLTSDTDATIKIPVNYDIHTSATPKII
                     TSKKSITLDELKNGFELDLEGIQGAQFNTDCVGNNVRLSLMHTANIPVMAMTYCDLDT
                     THIGFIIKALNDIPSTATEFTFRIEGNATTSQVPLLVKLPIVK"
     regulatory      complement(7390..7477)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
BASE COUNT         2900 a          922 c         1231 g         2692 t
ORIGIN      
        1 ggtgagacca gtgttgactc cagatttagg agcatgttta cttgctaaga agaaatatta
       61 tagtaaatag gtagaggtgg tagagtatga gttgcatttt aaaattaaaa cagatatatg
      121 aagatttaac agaagtagat aaaaaaatag ctgattacat acttaataat acagaggcta
      181 taagtaagct ttctgtatca gaattggcgt caaactcaaa aacgagtaca gcaagtatag
      241 ttaggttttc tagaaaaatg ggatattcgg gttttggaga tttaaaaatt gagatagcta
      301 aagatttgat gggcaaagaa aatgaataca catatgtaga tgaaaactat gattgtaata
      361 tagatagtgt tataaacaaa attacaaaca aaaacataga gactatcaac caaacaaggt
      421 tgttgaatga aaaagatata ataaaagaag cagttgaaca gataataaag gctaaaaatg
      481 tatacatatt tggagtaggt ggttcggctt tagttgcact agatttacag atgaaacttt
      541 taaggataaa taaacaggca tttacctctt tagatagtca tacacaactt atggtatctt
      601 caaatgtaga taaagaagat atatctatag caatatctta ctctggagaa tcaaaagaag
      661 taataaagtc tatagaaaat gcaaaattaa aaggatgtaa ggtcatttgt atgactaaat
      721 atagtgacaa tcatcttagt aagattagtg atttaaagct agtagtacca aatatagaaa
      781 aaaggcttag agaaggagct atttcttcaa gaatagcaat gcttacattg atagatataa
      841 tatatatttc tataattcaa gaaaacttaa atgaggcaga acagaaacta caagaaacaa
      901 aaaggatact tgattattta aatattgata atgcttaaaa ctaggttata tatcaactaa
      961 acaaataagt tagactataa gcgtaagtca ataagaaata tataaatatc taaaatattg
     1021 acttactcaa taataatata tagcatttta taatacaaat gagttgtctt aaaatttatt
     1081 ttttttaatc aaattttaag ttcaactcct tttttatatt attatagata tatcctatat
     1141 tttcccaaaa aacgtattga acatacaaat tataggaggt ataatgttat ggagagagaa
     1201 tatatgaaat gtatacaaaa ttgattttaa ctgtattatt gataattctc tttactctta
     1261 ccattactaa tgattgaaga cagtagtatt tatcataggt caaagattcc gaatatatta
     1321 atcatcatca ttttctcaaa ttaacagcaa acatactgat ttgcgagttg tattgtatac
     1381 aaattatcta gaaaatatct attagatttt ccatgaaatt taaatatttc gcgaacgtat
     1441 gaacgtagaa acatacatgt tgacaatcca catttttaaa tcccaaatga gctttattaa
     1501 tggtagagaa taggaagtaa caatatttta taaaggagaa tactaagtat gaagaaaatt
     1561 aggaagttag ctaccaagtt attgattaca acaatggttt taatctcagg tatgagtatg
     1621 acagtttatg gtatgacagc taaagaggta acagcaaaga cgccaaaaag ctacgtcact
     1681 ggaaccaata gcgtatatgg tccgaagttg tcacaagccc aactgaactc agtagcacaa
     1741 gcaacagcag attttatgaa caagaaaatc acaaagaaca tgactacaga tgctaagatt
     1801 ttagttgcct acaatcacat taaaaataac acaacatatg ttgactggaa tgcagtagag
     1861 ggagcaaata ctgcctatac attggttact aaaaaaggtg catgttcagg aatggctagg
     1921 agcatgaaag ctttgtgtga tgctatggga attgaatctt attatgtaca cagcacatca
     1981 aatgaccacc agtggaatct tatccgtttt ggtgatgggc gtttgtatca tgtagatatt
     2041 gatgctaata aatcagcagg aaaggatgtc atatacaaga gtcttagcca cccattacca
     2101 tttgataaga cagcatatcc aacagttgga gcagtagcac aaacgcaaaa aaaaccagca
     2161 ccacctggga agcacactat gttaccacac caagaagggg caaaggttgt tggaaaattt
     2221 ggagatataa aggataaaca tgtaatgtat gaaggtgttc gatgtgttgt aattaattca
     2281 tcagtaaata cagttacatt tatgggtagt gaatcaaatt tagacccatc agagcttacg
     2341 attgctgtaa taggatttat gaacacacca tatggaatgt ttgcattagc gccagaaagt
     2401 aaacccgttg aatatccttt tgaatatgat aagccatttt ctttagattt tagtggtaaa
     2461 aatcgttctg gtgcagaatt agcgaagtac tgtaaaaaaa atgatgtgac tattaaaata
     2521 gaaatcactg atgaaaaagc taatactgga cttggtcaaa taatgtatat tcgatatgaa
     2581 taatgattta atcatgacag tagaataagt tatgaacatt gcgccctctt actaggggcg
     2641 catttaatat tatatctgat attttgtagt caggtcaact gctggatttc tccccttttt
     2701 atttataaaa attattatct ctaagcatct gagcattttt acgattaact aaaaactgtt
     2761 ttaactcagt caagcatagg aataaaatag ccctattttc aaattctgtt ctgtcaacag
     2821 gtagaggttg gtttttcata ctaagcatta atctcttcaa ttcttcaaga agaagattgc
     2881 ctgtttcagc ttcaaattca gtatgaccta ttttatgtat gaaagcagag aggatatgag
     2941 cttgtggtgg tgtagaattt agttgagata cgtaactata caaattttct aagatatccc
     3001 gttgactttt tataatgtcc atatgttcta agaagaatct agtatcactc aataaagtat
     3061 tattaatatt atcgtaagtt tcagaaatac tgctgtcaat aagtgaattt aatgtatata
     3121 aatctctact atatccattt tcttttttag ggtttacaat aatatctgca atgtctataa
     3181 gtacaatact cacttcttcc tgtaactttt tttgaacttc atggatttta tgtatatttg
     3241 ttggaatata catatttatt aagacaccta ttccagttcc tactgcaaat attccagctt
     3301 cgtttaaaat ccacttaata gatgtttctc cttgaagaaa ataatgggag gcaatgacta
     3361 cacacatggc aataacatct tgaatattta ataaaaaaca aagtggtata attataaata
     3421 taaatactga aaatgaaatc aaattatatc ctaaaatatt aaaacataaa taggaaaaaa
     3481 tagtacataa aagaaaacca taaacctttc caatagagcc tttaagagtt tctcttttag
     3541 tatcttttac tgtaagtagt actataactc cagcaaccat tgaatattct aattttaata
     3601 gccaagcaat aaatatagca agagaacatc caagagacaa ttttattact ttaagtgaat
     3661 taatattatt caaatgcaaa cctcctttaa acaaatatag taaagataat tttagcataa
     3721 aactaatttt gtttaaaata tagttaacca tattttattt aaaaattatt atacaaatca
     3781 ataaaaagat aagtaagtgt ggatacaaag ataccttttt aatgtaaaat attgtagaat
     3841 acaaaatatt ataatgaaca caaaataatt ataatctatt aaataaaaaa tactttaaat
     3901 tacaattaga gtagttaata aaataactga aaatatatct attattatta acaaaaaata
     3961 aaattatata gttaatgtaa ttttataatt gatttggtat ggagattttt ttcttttgtg
     4021 ttatactatt tatgaaagca aatagaaaca ttaaaataca ttattaaata tgtatgagaa
     4081 gttttaagta gccttattaa tgaacttgat tattgttcat aatagcaaat tggaggagat
     4141 aaacatgaaa attactgaag gatatatgcc ttttgaaggt tttaaaactt attatcgtat
     4201 agtaggagaa aatacggaag ggaagaagcc attagtacta cttcacggag gtccaggttc
     4261 tacacacaac tattttgaag tgttagacaa gatagctgaa agtggtagac aagttataat
     4321 gtatgaccaa attggttgtg gtaattcatt tgtagaaggg catcctgagt tatttaatgc
     4381 agatacttgg ataaaagagc ttattgaact gagaaaacat ctagggcttg atgaaattca
     4441 tctgttaggt caatcttggg gagggatgca ggctatttgg tatgctattg aatataaacc
     4501 aaaaggtatt aagtcttaca tcctttcatc tactctttct tctgcaagac tttgggagaa
     4561 agaacaaaaa agaagaatat catatatgag tgagtctgac caaaaagcat tacttgatgc
     4621 agtaaatact ggagattact ctagtaaaga atacaatgat gcattagaaa gatttatgga
     4681 aatgtattgt gcaggtgaag tgacagaaga ttctccagag tgcctaagaa gacctaaaaa
     4741 atcaggttct gaagcatata ttgtaggatg gggacaaaat gaattttctc ctacaggaac
     4801 tctttctgga tatgagttta cagatagatt acatgaaata aaagagcctt gtctagtaac
     4861 tagtggagca agagatttat gttcaccata tattgcaaag actatgtatg atagaatacc
     4921 taatagtaag tgggagttat tcgagtattc aagacatatg ccattcgttg aagaaaatga
     4981 gaaatatata gaagtcttaa ctgagtggtt agatgctaac gattaaaaat aatataaatt
     5041 tatacaaaag agaggctaga catgttgtct agtctttttg gtatgataaa agtgtaatag
     5101 tttatattat aaacgatggt atatttgtac ttattttaaa atctattgtg tctaagaaaa
     5161 aattaatagt tgtagtaatg agtataattg taattaaata gtgagctttt tatatgttca
     5221 ttcaagaata aatgattcat catgaggaaa tcatgataat aaaaataaat attgttcagg
     5281 aagttatcac tatccattgt ggtagttatc cagttcattt acatgataat ggagtatcct
     5341 tataatggag gatagaagta tctgtagagc aacaaccttt tttatataca atatatatat
     5401 aaagtaatat atattgtata taaaatttat tatgatttac taagatttct aattgacatt
     5461 atatgtattt agaggtacta ttatattaat ttaaatttaa taactattag aataaatatt
     5521 tattccgaaa agatgcttta aagtaaatta catttttatt tataataaga gatgcaagtg
     5581 taatgtttta gatataataa ttacaagtat atataaatta tactaatggt tttatagtag
     5641 ctaagtgttt atatggtaat aaaaattgta attgtaattt atgatggtga taatacataa
     5701 atgaggttgg caatgataag cttatgtagt ttattaaata aaaattatta ataatcaatt
     5761 atcaatgatt attttaactt atgcttttgc attatatgtg taaatataac aattgaagat
     5821 atttggtatt tgaaatttct agttttatga gaaacataat atcgctcaga taaatttgtt
     5881 atgacattac aagaattttt aaaggtatat tgcttaaact taaaggcaaa gatatattgt
     5941 cagataaagc agtatctatt aaagatgaaa tttttattaa agagcaagta gaaactgtca
     6001 tatatcttca aatttagtaa gtattatttt gtgaatatct ttgcctttat taatttgtat
     6061 tgattgtttt tcaattaaat cttctattat agttgattat atgaaaaatt ctgtattatt
     6121 tagtaatcat aatatagttt agtttctctg cttattgtat aagtttttaa agcagatgta
     6181 atgtagctta gttatagtca tagggataac agtattagct tttaaagttt atattatatg
     6241 tttaaagttt tattttacaa taggaagctt cactagtaat ggtacttgag aagttgtagc
     6301 atttccttct attctgaatg taaattctgt tgctgttgaa ggaatatcat ttaaagcttt
     6361 tattatgaaa ccaatgtgtg ttgtatcaag gtcacagtat gtcatggcca ttacaggaat
     6421 attagcagta tgcattaagc taaggcggac gttgttacct acacaatctg tattaaattg
     6481 agcaccttgt ataccttcaa gatctaattc gaagccattt tttaattcat ctaatgttat
     6541 acttttttta cttgtaataa ttttaggtgt agctgatgta tgaatatcat agtttacagg
     6601 tattttaata gttgcatcag tatctgatgt tagaacagaa gaattaatgt taatttccca
     6661 gtttggtaag tattcaatac cagcattagg ttcatatact ccaaattcaa tggattttcc
     6721 atctgtacct acagctattg ttgtttgtac atcatagttt tgttttattg catttttaat
     6781 ttcatatgga tcatttgtgt tgaactttgc accttcaagg gtaagcttta cccattgttc
     6841 tccaccacca tttttaaaaa agttcgtgta gttaattgat gactggtcgc atttaagaag
     6901 aacatttgaa gatattcgtt gttctattga agttaaattt tgtgtttgga ttgcttgaga
     6961 tgcaaacact gggtaagtat ttgtagctaa tactcctgta attagtagtg atgtgataat
     7021 tttttgcttt ttcatttttg tttcctccta taatatgtta tatttttaac agtgaaatga
     7081 aataaaatac aagggcaata taaaatatat gtcgttttga agtgtattaa gattgtgtga
     7141 aatattatta gttatactga aatatactag tctatagata tacatagttt aaaataagta
     7201 aagtagtatt actgtcttac ttaacactcg tttttaatcc ataaaataat gttttcagag
     7261 ttaaataatt atctttcaaa tctaatctca attctttagc atagaggtta tagatacaat
     7321 aataagaata tggctagaac aaaaaaatac ctcataagga ggtattatct aacaaaaagt
     7381 acactaactt ttagtaactg gcaggcataa tctttttaag accttaaccc ctatagcttt
     7441 gcgtccctag atttctctag gtttgcctat taagttttgc gtttgcattt ttaatatcat
     7501 atcaatctgt tttatagaat gataacacaa ataaaactta taatcaacaa ttacatgaaa
     7561 tttttggaaa aaaattatat attacaaaaa taatattttc ttcatgaata atttgttaat
     7621 ataaactata ttatttataa ttttatagtt tttattgatg tgtttttgtt tattgttgtt
     7681 aaaataatta ttactatcaa atcatttaaa attttcataa tcaataattt tttatacatt
     7741 ttgtc
//
LOCUS       DAFNNB010000160         7695 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0165, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000160 DAFNNB010000000
VERSION     DAFNNB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7695
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0165"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            142..2235
                     /locus_tag="L3M60_003066"
     CDS             142..2235
                     /locus_tag="L3M60_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6227700.1"
                     /translation="MNKHNFEVILNQLQINIYVTNIHTNEIVFMNKKMKEEYNILDPE
                     GKVCWQVLYPEKNSTCSFCKVVELLKNDKKGVLIKWYEKCNKLNCVFENYDSLITWQD
                     GTVVHMHQSIDITNSTSLNKQIKPNEFHKISNTEEEKGVFNVSIDNFDYNSTLLYDAL
                     IRGTDEYIYICNMKTGVFRYSPSQVELFDLPGEIVENPLIYWEKIVHPEDWKRFYKSN
                     TEIGKNQMDYHTVEFRAKNRSGEYIWLRCRGQLMRDEFGKPSIFAGIMTQLGKQNKID
                     SLTQLLNYHEFMSVFEDKISNPMIEKLCIVLLNIDDFKNVNEMYDRDFGDNIIKTLAQ
                     SIQSILPDNAELYKLDGDEMGIIVDNVEEKEILTLYNQIQNMIIHLQLWRKYGLNITI
                     SAGCVIYPKHGDTVKELYKYASYSLQYAKEHGKNRLVFFSQEILKNKMYSLEMMRDLK
                     ASINDDFRGFSLRFQPQVDTQSHKIIGVEVLLRWTNDKYKAISPLEFIPILEEHDMIN
                     TVGAWVLRMALRTFKGLIDYYPFFKVSVNVSAVQILEDTFIEDIVKIIDDEKFPYQNL
                     VLELTESHTVQNMSILQFKFKALQDLGIYIAMDDFGTGYSSLEVLKFSPIDVVKIDRV
                     FVKDILKSKFDATFIHFIVAICHDVGIKVCLEGVETQEEYDLVKQMKPDYIQGYLFGK
                     PQTATEIFDLLKLDN"
     gene            2689..3507
                     /locus_tag="L3M60_003067"
     CDS             2689..3507
                     /locus_tag="L3M60_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6227701.1"
                     /translation="MEKQYFTTGEFAKLCGISKQTLIFYDKIGIFSPEYKDKNNYRYY
                     SVYQYDTLDILQSLREIGMSLEEIKEYIQNRTPQLCVKMLKEEEKKIREKIKKLRKIS
                     SKMQNRINITVEGISKMNSEEIYISKKSEEYLVLSSDLSNMSNGDFMMELIDFTNYCK
                     AKNLYQGYELGVIVSNENLKNGDYLSISSFYLKIDKKIKDKRLYIKPEGMYACIKHIG
                     KYENSYKSYEKLKKYIYENEYKIIGNSYEESILDFFCESNEDNYMTEISIQVSR"
     gene            complement(3620..5740)
                     /gene="feoB"
                     /locus_tag="L3M60_003068"
     CDS             complement(3620..5740)
                     /gene="feoB"
                     /locus_tag="L3M60_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBN6227702.1"
                     /translation="MGLTHNSTKMSSLKDMFDIDNKEDQFVIALAGNPNTGKSTVFNH
                     LTGLRQHTGNWPGKTVATARGNFKHKNTEYALIDLPGTYSLFALSQEEIVARDFICFG
                     NPDAVIVVCDATCLERNLNLVFQVMELTDKVILCINLIDEAIKKGITIDKKLLEDSLC
                     IPVILTAARNGSGMDELLDTLNDVSFDKYKLNNKPVRYNENIENVVKSIQPELDNIIP
                     GINSRWLGLRLIDGDESIFESMSNYIDKDSIDAINEVKKKIPDNINKQKIRDEFTKIN
                     YDYAKKLSDECCSNVAKKSTDREEKVDKILTSKIFGLPIMLLLLGTILWITIEGANYP
                     STLLSNLLLGFEPSISGILNSINCPSWLNDMLVLGLYRTLAWVISVMLPPMAIFFPLF
                     TLLEDFGYLPRVAFNLDHLFKKACAHGKQCLTMCMGFGCNAAGVIGCRIIDSPRERLI
                     AILTNNFVPCNGRFPTLIAISTIFFSSVITNSFVSSVATALCITLLIILGVIITLLVS
                     YTLSKTLLKGVPSTFTLELPPYRVPQIGRTLYTSIIDRTIFVLGRAVMVAIPAGVITW
                     IFANIYIGDLSILSHVANFLDPLAKLIGLDGFILLAFILGFPANEIVVPILLMAYLAT
                     GSMIELDSFSALGQVLREHGWTYLTALNVMLFSLLHWPCATTLLTIKKETGSLKWTAL
                     GFLMPTILAFVVCFLTTTVYNLFI"
     gene            complement(5766..5993)
                     /locus_tag="L3M60_003069"
     CDS             complement(5766..5993)
                     /locus_tag="L3M60_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBN6227703.1"
                     /translation="MKNLNDIQVKKTVKVEDILSSGNLRERMLALGLTRGAVIDVVRK
                     GPKNNLTVYKIRGSKIALRQEESSLILVSDI"
     gene            complement(6320..6760)
                     /locus_tag="L3M60_003070"
     CDS             complement(6320..6760)
                     /locus_tag="L3M60_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227704.1"
                     /translation="MFKIKHFNDLNLDEFYEIAKSRYEVFACEQKIFSLNDYDDIDKN
                     SYHIFLKENGLICAYARIIPKEYSSYNDVSIGRVLVLSRHRRKGLAKQMMDCAIDFIK
                     VNLHENNITLSAQTYIKNLYLSCGFKEISEVYDEAGIEHIKMRL"
     gene            6912..>7695
                     /locus_tag="L3M60_003071"
     CDS             6912..>7695
                     /locus_tag="L3M60_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HBN6227705.1"
                     /translation="MIYGGSVLNTTKKLTEAALLSSLFIVVTIISVSTGFGYAIYLDF
                     IVPIFFCIICLKCDLKYTILSGITSLLIISLVLGNLGTAIWATQSVLLGIMCGYLINK
                     PTMVMDDLVYGSVFGVIVMVFIDIYASTLIGYSFMKEFQGYSKWIYFNGYTNFIYYLM
                     IALFPFGMVFCIYLLSLILGNKLHILDSNSLKKLLIFKNFRTLNQFLCCSKKVFYICV
                     SYLTIFEVVKLLNVKVDFVYLKTVFISIAYLCVYFIIRDSCMS"
BASE COUNT         2922 a          944 c         1118 g         2711 t
ORIGIN      
        1 tataatttaa aatatgaagt aataaatttt ctaattaaat taaggtattg tgtatatata
       61 taaattataa gtaaatcttc tggaaagaat aattttaaca atgttgttat aaagagcaat
      121 aactatggag gagataagta tatgaacaaa cataattttg aagttatatt aaatcaacta
      181 cagattaaca tatatgttac gaatattcat actaatgaaa ttgtttttat gaataaaaaa
      241 atgaaagaag aatataacat tttagatcca gaaggaaaag tttgttggca agtattatat
      301 cctgaaaaaa attctacttg tagtttttgt aaagttgtag aattgttaaa gaatgataaa
      361 aaaggagtat taataaaatg gtatgaaaaa tgtaataagc taaattgtgt ttttgaaaat
      421 tatgatagtt taattacatg gcaagatggc acagttgttc atatgcacca atcaatagat
      481 attacaaact caacaagctt aaataaacaa attaagccta atgaatttca taaaatatca
      541 aatactgaag aagaaaaagg agtttttaat gtctcaatag acaattttga ttataattct
      601 acattgcttt atgatgcttt gattagaggt acagatgaat atatatatat ttgtaatatg
      661 aaaacaggag tatttcgata ttcaccatca caagttgaat tatttgactt accaggagaa
      721 attgttgaaa atcctcttat ttactgggaa aaaattgtac atccagaaga ctggaaacgt
      781 ttttataagt caaatactga aataggtaaa aaccagatgg actatcatac agtggaattt
      841 agggctaaaa atcgcagtgg agaatatata tggttaagat gtagagggca actaatgcgt
      901 gatgaatttg gaaaaccaag tatttttgca ggaataatga cacaattagg taaacaaaat
      961 aagattgatt cattaacaca gttattgaac tatcatgaat ttatgagtgt atttgaagat
     1021 aaaatttcaa atcctatgat tgaaaaatta tgcatagtat tattaaatat tgatgatttt
     1081 aaaaatgtta atgagatgta tgatagagat tttggagata atataataaa aacactagca
     1141 cagtctatac aatctatatt accagataat gctgaacttt ataaacttga tggagatgaa
     1201 atgggaatta ttgttgataa tgttgaagaa aaagaaattc taactttata caatcaaata
     1261 cagaatatga ttatacacct tcaactatgg agaaaatatg gattaaatat aacaatttct
     1321 gctggttgtg taatatatcc aaagcacgga gatacagtaa aagaacttta taaatatgct
     1381 agttactctt tacaatatgc aaaagagcat ggtaaaaaca ggcttgtttt tttctcacaa
     1441 gaaatattaa aaaataaaat gtattcatta gaaatgatgc gggatttaaa agcatcaatt
     1501 aatgatgatt ttaggggatt tagtcttcga ttccagccac aagtagatac acaaagtcat
     1561 aaaataatag gtgtagaagt ccttttaaga tggactaatg ataaatataa agcaatttct
     1621 cccttagaat ttattccaat attagaagaa catgatatga taaatactgt aggagcatgg
     1681 gtgcttagaa tggcgttacg tacttttaag ggattaattg attattatcc tttttttaaa
     1741 gtgagtgtaa acgtttcagc tgttcaaata ttagaagata catttattga agatatagta
     1801 aaaatcatag atgatgaaaa gtttccttat caaaatcttg ttttagaatt gacagaaagt
     1861 catacagtac aaaatatgag tattttacag tttaagttta aggctttaca agatttggga
     1921 atttatattg ctatggatga ttttggtact ggatattcgt ctttagaagt tttaaaattt
     1981 tctccaatag atgtagtgaa aattgataga gtatttgtta aagatatatt aaagagtaag
     2041 tttgatgcta catttataca ttttattgtt gcaatttgtc atgatgtagg aataaaagta
     2101 tgtttagaag gtgtagaaac acaagaagag tatgatttag ttaaacaaat gaagccagat
     2161 tacatacaag gatatttatt tggaaaacca cagacggcaa ctgaaatttt tgacctttta
     2221 aagctagata attaattcta atttatcaat aatattactg aaaagcttta aaatcaaaaa
     2281 tacattagat tattatattg aagatatatc aattataaat tgtgatataa gaaaatagca
     2341 cttcaatagg tgttaaattg gaatattcat atgcttaaag gaatctaaaa agagataact
     2401 agaataaaaa tagatatctc tttttagatt aaaaagtaat atatcataaa taaaatagta
     2461 agaggtagat acaatttaca atttccgctc taacaagcat aaatataata tctaaaagtt
     2521 cagagggtgt atatttttta ttactgggat ggataaaata tttaactgac atttaatctt
     2581 taaaatcaaa aacatattga ttttctaaaa taataactac ttgatttagt aaatactaaa
     2641 gtatatagta actatatata taataaaaat aaaaggagtg cataaccctt ggaaaaacag
     2701 tattttacaa caggtgaatt tgctaaattg tgtggtatat ctaaacagac attaattttc
     2761 tatgataaaa tagggatttt ttctcctgaa tataaagata aaaataatta tagatattac
     2821 tcagtttatc aatatgatac attggatata ttacaatcat taagagaaat aggaatgagt
     2881 ttagaggaaa taaaagagta tatacaaaat agaactcctc agctttgtgt gaaaatgctt
     2941 aaagaagaag aaaaaaagat aagagaaaaa ataaaaaaat tacgaaagat aagttctaag
     3001 atgcaaaata gaataaatat aacagtcgaa ggaatttcta aaatgaatag tgaagaaatt
     3061 tatatatcca aaaagtctga agaatattta gttttatcaa gtgatttgag taatatgagt
     3121 aatggtgatt ttatgatgga attaatagat ttcaccaatt attgtaaagc gaagaattta
     3181 tatcaaggat atgagttagg agtaatagta agtaatgaaa acctaaaaaa tggggactat
     3241 ttaagtatat caagttttta tttgaagata gataaaaaaa taaaggataa aaggttatat
     3301 ataaagccag aaggaatgta tgcttgcata aagcatattg gaaagtatga gaattcatat
     3361 aaatcttatg aaaaattgaa aaaatatata tatgaaaatg aatataaaat tattggaaat
     3421 tcatatgaag agagtatact tgactttttt tgtgaaagta atgaagataa ttatatgact
     3481 gaaatttcaa tccaagtgtc cagataagag ttaaaataga gatatatcca aatactaagt
     3541 aattttactt atcattgaat atatctctat gctttataat aaagtattct aatatatgtc
     3601 taatgtcagt taatgttaat tatataaata aattgtaaac tgtagttgtt aaaaaacaaa
     3661 caacaaaagc aagtatagta ggcattaaga atcctaaggc agtccatttt aaactaccag
     3721 tttccttttt tattgttaaa agtgttgttg cacaaggcca atgtagtaaa ctgaacagca
     3781 taacatttaa agctgtcaaa taagtccatc catgttctct aagaacttga cctaaagcac
     3841 taaagctatc taattctatc atagaaccag ttgctaggta agccattaaa agtataggta
     3901 caactatctc atttgctggg aatccaagaa taaaagcaag taatataaaa ccatcaagac
     3961 caattaactt agctaaaggg tctaaaaagt ttgcaacatg tgataatatg cttaaatctc
     4021 ctatatatat atttgcaaat atccaagtta tgacaccagc aggtatggct accattacag
     4081 ctcttcctaa aacaaatatt gttctatcta ttatagaagt atataaagtt cttcctattt
     4141 gaggaactct ataaggtgga agttctaatg taaacgtaga tggaactcct tttaatagtg
     4201 tcttagaaag agtataagaa actaataatg ttattataac acctagaatt atcagtaaag
     4261 ttatgcaaag tgcagttgct acgcttgata cgaaagagtt ggttataact gagctaaaaa
     4321 atattgtaga tatagctatc aaagtaggaa aacgaccatt acaaggaaca aagttgtttg
     4381 taagtatagc tataagtctt tcccttggag agtcaattat cctacaacca ataactccag
     4441 ctgcattaca tccaaatccc atacacatag tcaagcactg ttttccatgt gcacaagctt
     4501 ttttaaatag gtggtctaag ttaaatgcaa ctcttggcaa atatccaaaa tcctcgagta
     4561 aggtaaataa tgggaaaaat attgccatag gaggtagcat tacagaaatt acccatgcta
     4621 atgttctgta taatcctaaa accaacatat catttaacca tgatggacaa ttaatactat
     4681 ttaatatacc cgaaatactt ggctcaaatc cgagtaacaa attagacaat aaagtggatg
     4741 gatagttagc accttctatg gtaatccaca atatggttcc taataataag agcattattg
     4801 gaagtccaaa tattttagat gttaatattt tatctacttt ttcttctcta tcagttgatt
     4861 ttttagcaac attagaacaa cactcatcac tcagtttttt agcataatca tagttgattt
     4921 tggtaaactc atctcttatt ttctgtttat ttatattatc tggaatcttt ttctttactt
     4981 cattaatagc atctatggaa tctttgtcta tataattact catagattca aatatacttt
     5041 catcaccatc aataagtcta agtcccaacc atctagaatt tatacccggt ataatattat
     5101 ccaattctgg ttgaattgat tttaccacat tctctatatt ttcattgtat ctaacaggct
     5161 tattatttaa tttgtatttg tcaaaagata catcatttaa agtatccaat agctcatcca
     5221 taccagaccc gtttcttgca gcagttagaa taactggaat gcaaagagaa tcctctagaa
     5281 gttttttatc aatagtaatg ccttttttta tagcttcatc tattaaatta atgcataaaa
     5341 taactttatc tgtaagttcc atcacttgaa atactaaatt taaatttctt tcaagacatg
     5401 tagcatcaca tactactatt actgcatctg gattaccaaa acatataaaa tcgcgagcta
     5461 caatctcttc ctgtgataag gcaaataaag aatatgttcc tggtaaatct attaatgcat
     5521 attcggtatt tttatgttta aaattaccac gagctgtagc tacagttttt cctggccagt
     5581 ttccagtatg ctgtctaagt cctgtaagat gattaaatac agtactttta cctgtatttg
     5641 gattaccagc caatgcaatt acaaactggt cttctttgtt gtctatatca aacatatctt
     5701 ttaaagaact cattttagtt gagttatgtg ttaaccccat attaatatcc tcctgctaga
     5761 aatttttaaa tgtcagagac taatattaag ctactttctt cttgtcgtag agctatctta
     5821 ctacctctga ttttatagac agttaaatta ttttttggac cttttctaac tacatctata
     5881 acagctcctc tagtaagacc aagcgctaac attctttctc ttaaattacc actagataat
     5941 atatcttcaa ctttgacagt ttttttcact tgaatatcgt taaggttttt catagtagca
     6001 cctccatgct tggaatttag cctgggctaa tcttcttatt ctaaactatg cattatatta
     6061 tcaccttgtt acttttacaa aaaaaagata ggcaagatgc ctatcataca aagttgggta
     6121 taattcttac ttttggggaa agtagaatat agaataaaaa ttaacacttt gaatccatat
     6181 aattattgaa ttttgataat aaaaaagcca gaaagcaaaa gcctcctgac tctatgtaag
     6241 gtattttcaa atacctaaat atcaattcaa tatttaattt ataattaaat tatatattaa
     6301 caatattagc ttgtcaacac tataatctca ttttaatatg ttcaatacct gcttcgtcgt
     6361 atacttctga gatttcttta aaaccacaag ataagtaaag gttctttata taagtttgtg
     6421 ctgataaggt tatattgttt tcgtgtaagt taacttttat aaaatctata gcacaatcca
     6481 tcatttgttt tgctaaacct tttcttctat gacgagaaag aactaaaact ctaccaatgg
     6541 aaacatcatt ataagagctg tattctttag gaattattct agcataagca caaatcaatc
     6601 cattttcctt taagaatata tgataagagt ttttatctat atcatcataa tcgtttaacg
     6661 aaaatatctt ctgttcacaa gcaaatactt catatcttga ttttgctatt tcataaaatt
     6721 catctaaatt taaatcatta aagtgtttaa ttttaaacat aataattacc tcctagtatc
     6781 ttaaaaaact gaatattaag atacattata tcatttttaa cataaatacc tttattaaaa
     6841 tttatattta ttaaaaatat ttttaaaaag tgatatacta tattaaaaac aaaactttag
     6901 ttagaaaatt tatgatttat ggaggtagcg ttttgaatac tactaaaaaa ttaactgaag
     6961 cagctttatt gtcatcttta ttcatagtag taacaattat atctgtgagt actggatttg
     7021 gatatgctat ttatttagat tttatagtgc caatattttt ttgtataatt tgcttgaaat
     7081 gtgatttaaa gtatacaatt ttatctggaa taacttcact tttaataatt tctctggtac
     7141 ttggaaattt aggaactgct atttgggcaa ctcagagtgt attacttggt ataatgtgtg
     7201 gctatctaat aaataagcct acaatggtta tggatgattt agtatatgga tcagtgtttg
     7261 gagttattgt aatggttttt attgatatat atgcttcaac attaataggt tatagtttta
     7321 tgaaagaatt tcaaggatat agcaaatgga tttattttaa tgggtataca aattttattt
     7381 attatttaat gatagcattg tttccttttg gtatggtgtt ttgcatatat ttattaagtt
     7441 tgattttagg gaataaattg catattttag attctaattc tttaaaaaag cttttaatat
     7501 ttaagaattt tagaacttta aatcaatttt tatgttgttc aaagaaagtt ttttacatat
     7561 gtgtttctta tctaaccatt tttgaagttg taaagctgtt gaacgttaaa gttgattttg
     7621 tttatttaaa aacagtcttc atatcaatag cgtatttatg tgtctatttt attattagag
     7681 actcatgtat gtcat
//
LOCUS       DAFNNB010000161         7678 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0166, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000161 DAFNNB010000000
VERSION     DAFNNB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0166"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(147..290)
                     /gene="scfA"
                     /locus_tag="L3M60_003072"
     CDS             complement(147..290)
                     /gene="scfA"
                     /locus_tag="L3M60_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HBN6227706.1"
                     /translation="MKNKHVKTLSQATLKQSAAKGGCGECQTSCQSACKTSCTVANQE
                     CER"
     gene            complement(356..718)
                     /locus_tag="L3M60_003073"
     CDS             complement(356..718)
                     /locus_tag="L3M60_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HBN6227707.1"
                     /translation="MGKSNYIFKGLGYAYIITLAVLLVYNLFLTFTDIGGDNITMVSS
                     FITTISAAIGGFYTSKHMKEKGLMYGLLVGLLYIVCIFLTVFLAQEKFVFEVGMIYKL
                     LLISAAGGIGGVLGVNFK"
     gene            complement(776..1069)
                     /gene="yajC"
                     /locus_tag="L3M60_003074"
     CDS             complement(776..1069)
                     /gene="yajC"
                     /locus_tag="L3M60_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBN6227708.1"
                     /translation="MPQQQIIMSIGLWVVVIAIFYFLMIRPQKKKDKQLKEMRSSLGV
                     GDKVVTIGGIVANVAKVEDDVVILELGPNRTKVPFEKWAIGTVKSKKEEIEED"
     gene            complement(1203..2324)
                     /gene="tgt"
                     /locus_tag="L3M60_003075"
     CDS             complement(1203..2324)
                     /gene="tgt"
                     /locus_tag="L3M60_003075"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBN6227709.1"
                     /translation="MYAVRYELIKTCKQSGARLGRLHTPHGIIETPIFMPVGTQATVK
                     SMTPEELKEIGSQIILSNTYHLYMRPGHELIKRAGGLHNFMNWDKPILTDSGGFQVFS
                     LGPLRKIKEEGVEFRSHLDGSKHFLTPEKAMEIQNALGSDIMMAFDECAPYPADREYV
                     KNSLERTTRWLKRCKDAHNNTDKQALFGIIQGGMYKDLREQSAKEITSIDLPGYAIGG
                     LSVGEPKPLMYDVLEHTTPLMPKDKPRYLMGVGSPDDLVEGVIRGVDMFDCVLPTRIA
                     RNGTAMTSQGKVVVRNATYAEDFTPLDPECDCYACKNYSRAYIRHLIKANEILGARLI
                     TTHNLHFLLNLMKQIRQAIMEDRLLDFRNEFFAKYGYEI"
     gene            complement(2403..2888)
                     /locus_tag="L3M60_003076"
     CDS             complement(2403..2888)
                     /locus_tag="L3M60_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227710.1"
                     /translation="MHSLQKQKKNNPILKIAFIFERVLAVVVLIAVFLGTIDVLRLMW
                     GAYIVDFQNPVQYSQLNDFLAQILLLVIGVELVVMLSLHIPGAFIEALLYAIARKMLL
                     LPKNEGMIDVLIGVIAIAGLFAIKKFLVTKDKSALNIMSIHDEEAIKAQQNKEDEDKS
                     I"
     gene            complement(2900..3925)
                     /gene="queA"
                     /locus_tag="L3M60_003077"
     CDS             complement(2900..3925)
                     /gene="queA"
                     /locus_tag="L3M60_003077"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBN6227711.1"
                     /translation="MKTSDFKFDLPQELIAQVPIEDRASSRLMVLDKETGNIEHKVFR
                     DIIEYLNPGDCLVLNNTRVIPARLMGEKLETGGKIEFLLLKRTEEDTWQALVKPGKRA
                     KVGTKFSFGNGKLIGEVVDLSDEGSRIIKFHYDGIFEEILDELGNMPLPPYITARLDE
                     KERYQTVYSKHNGSAAAPTAGLHFTEELLSKIKEKGVDIAFVTLHVGLGTFRPVKVED
                     VLNHKMHSEYYMVSQEAADKINRAKENGNNVICVGTTSCRTIESACNEDGKMKETSGW
                     TEIFIYPGYKFKVLDKLITNFHLPESTLIMLVSAICGKDNVLNAYNEAVKERYRFFSF
                     GDAMIIK"
     gene            complement(4243..5262)
                     /gene="ruvB"
                     /locus_tag="L3M60_003078"
     CDS             complement(4243..5262)
                     /gene="ruvB"
                     /locus_tag="L3M60_003078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBN6227712.1"
                     /translation="MQGFEDENRIITSTMKVEDIDIENSLRPKTLEDYLGQEKSKEQL
                     SIFIEAAKSRNEQLDHVLLYGPPGLGKTTLASIIANEMGVNLRITSGPAIERAGDLAA
                     ILTNLNENDVLFIDEIHRINRSVEEVLYPAMEDFCLDIIIGKGPSARSIRLDLPKFTL
                     IGATTRAGMLTNPLRDRFGVICKLDYYTVDELSKIVLRSSNILDAKIQSNGALELAKR
                     SRGTPRIANRLLKRVRDFAQVRADGKITDKVAKDALELLGVDSLGLDFVDEKLLMTII
                     EKFRGGPVGLDTLAASIGEDRNTIEDVYEPYLLQLGFINRGPRGRVAMPLAYEHLKIP
                     YPNEK"
     gene            complement(5369..5971)
                     /gene="ruvA"
                     /locus_tag="L3M60_003079"
     CDS             complement(5369..5971)
                     /gene="ruvA"
                     /locus_tag="L3M60_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBN6227713.1"
                     /translation="MYSYIKGTVEEIYIDSIVVENNGIGYKINVSSNTIMNLQVGEAT
                     KIYTKLIVREDDMSLCGFVSREELKMFELLTSVSKIGPKVALSILSFASPAQLGAYIL
                     SEDIGKLSKAPGVGKKTAERIVLELKDKVDKNNIEFEPTLLSQKPTLISQDESVDALV
                     ALGYTLAESKEAVQKCKKDGMNTEAIIKKALTYIMSKSLK"
     gene            complement(5964..6473)
                     /gene="ruvC"
                     /locus_tag="L3M60_003080"
     CDS             complement(5964..6473)
                     /gene="ruvC"
                     /locus_tag="L3M60_003080"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBN6227714.1"
                     /translation="MIILGIDPGIAIVGYGIIEYKNSKFKVIDYGAVTTPAHMNISRR
                     LELVYKGIDTIVKNYNIDEVGMEELFFNKNVKTAITVAQARGVTMLACAHNGKPVYEY
                     TPLQVKQGVVGYGRADKAQVQQMVTSFLSLKKVPKPDDVADALAVAICHAHSNKLEKT
                     LKNIGGKYV"
     gene            complement(6785..7414)
                     /locus_tag="L3M60_003081"
     CDS             complement(6785..7414)
                     /locus_tag="L3M60_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227715.1"
                     /translation="MFEKKKKELFPWKISALILVGVFVLSSGVYIGTRLKHNGVQTGE
                     VVSTSANMNEKSKEAFGLNKNCEVWLQSEGENETNYKRTLTMAGIVPESLLDKTKSEI
                     VSYFKKEYPTKKIKSMDKNEIILVESKYSDNKDVQAMDVTIKGKYTIENDNGNIALYK
                     YDDTGSKTLVEKTKIRVDSLPKTVQDEIKKGVVMDTEEEAYSRLEDFAS"
BASE COUNT         2386 a         1444 c          837 g         3011 t
ORIGIN      
        1 cataattaaa cattaagaaa tcaaatttaa ttttatataa atagtttaaa tattttatat
       61 acaaacaaaa ataaaagagg atacctccta atgataaaag aatcacattg agataccctc
      121 ttacaaacaa ctaaatcaat aattttctat ctttcacact cttgatttgc aactgtacaa
      181 gaagttttac aagctgattg acaagaagtt tgacactctc cacatccacc ttttgcagca
      241 ctttgcttta aagtagcttg agataaagtt tttacatgtt tatttttcat tttaaacgcc
      301 tccatttcta attgtttcct attgattata tcataaaact accttaatat aaactctatt
      361 taaagttgac acccaatacg cctccaatgc ctcctgctgc tgatattagc aataacttat
      421 aaatcattcc tacttcgaat acaaactttt cttgtgctaa aaacacagtc aaaaaaatac
      481 atactatgta caaaagtcct actaaaagcc catacataag tcctttttct ttcatgtgtt
      541 ttgaagtata aaaccctcct atagctgcag aaatagtagt tataaatgat gaaaccatag
      601 ttatattatc cccaccaata tctgtaaatg tcaaaaataa gttatacact aataaaacag
      661 ccaaagttat tatatatgca tatcctagac ctttaaaaat ataattagat tttcccataa
      721 aaaaacctcc tctcaaatta atatatattc gagaaaaggc ttttaaatta ctaatttagt
      781 cttcttctat ttcttctttt ttagatttaa cagttcctat agcccatttt tcaaatggta
      841 ctttagttct atttggtcct aattctaata ttacaacatc atcttcaact ttagctacat
      901 tagctactat tccacctatt gtaacgacct tgtctcctac acctaggcta cttctcattt
      961 cttttaattg tttgtctttt ttcttttgag gtctaatcat taggaaataa aagattgcga
     1021 ttactactac ccatagacct atactcataa ttatttgttg ctgtggcatt aatttttcac
     1081 tcctttgtaa attatactct tttgtaaatc ataataaatg tttatataac aatttacttt
     1141 ctctatcttt gagtactatt gaatattata tattaaaatg attataatta atagtgcaaa
     1201 atctatattt catatccata ttttgcaaaa aattcatttc taaaatcaag taatctatct
     1261 tccattatcg cctgtcttat ttgtttcatt aaatttaata agaagtgaag gttgtgagta
     1321 gttattagtc ttgcacccag tatttcattt gcttttataa ggtgtcttat ataagctctt
     1381 gaatagtttt tacaagcata acaatcacat tcagggtcaa gtggagtaaa gtcttcagca
     1441 taagttgcat ttctaactac tactttacct tggctagtca ttgcagttcc atttctagcg
     1501 atacgagtag gtaagacgca atcaaacatg tctactcctc ttataacacc ttccactaaa
     1561 tcatctggac taccaactcc cattaggtac cttggtttat cctttggcat aagtggagtt
     1621 gtatgctcca gcacatcata cataagtggt tttggctctc caacactaag cccaccaata
     1681 gcatatcctg gtaagtcaat actagttatc tcttttgctg attgctctct taaatctttg
     1741 tacatcccac cttgtattat accaaataaa gcttgcttat ctgtattatt atgggcatct
     1801 ttacatcttt ttaaccatct tgttgttctt tctaaagaat tttttacata ttctctatct
     1861 gctgggtatg gtgcacactc atcaaacgcc atcattatat ctgaacccaa cgcattttgt
     1921 atttccatag ctttttctgg agttagaaaa tgttttgaac catctaagtg tgacctaaat
     1981 tccacacctt cttcttttat ctttcttaaa ggacctaagc taaacacttg gaatcctcca
     2041 ctatctgtaa gtattggttt atcccaattc ataaaattat gaagaccacc agctcttttt
     2101 ataagttcat gacctggtct catatataaa tggtatgtgt tactaagtat tatttgtgaa
     2161 ccaatctctt ttaattcttc tggtgtcata gattttacag ttgcttgagt tccaactggc
     2221 atgaatattg gcgtttcaat aattccatgt ggagtgtgta atcttcctaa ccttgcgcca
     2281 ctctgcttac aagtctttat caattcgtat cttactgcgt acataaagtt ctccttaatc
     2341 atatttattt ctcattattt cttaagatat atttgtaata agctattcat ataatttatt
     2401 gtctaaatag atttatcttc atcttctttg ttctgttgcg ccttaattgc ttcttcatca
     2461 tgaatactca ttatattaag agcactcttg tcttttgtta ctaaaaactt ttttatagca
     2521 aacagccctg caatagctat aactcctatt aaaacatcta tcataccttc atttttaggc
     2581 agtaacaaca tttttctagc tatagcgtaa agtaatgcct caataaatgc tcctggtata
     2641 tgaagagata acattactac aagttcaaca cctatcacta agagtaatat ttgtgctaaa
     2701 aaatcattaa gctgactgta ttgtactgga ttttgaaaat caactatata cgctccccac
     2761 ataagtctta gaacatctat agttcctaaa aatactgcta ttagaactac tactgctaaa
     2821 acacgttcaa atataaaagc aatctttaat atagggttat tctttttttg tttttgtaaa
     2881 ctatgcatca attacctccc tactttataa tcatagcatc tccaaaactg aagaatctat
     2941 atctttcttt tactgcctca ttatatgcat ttaaaacatt atcctttcca caaatagcac
     3001 ttactaacat tattagtgta gactctggca aatgaaaatt agtaataagt ttatctaaaa
     3061 ctttaaattt ataacctggg tatataaata tttctgtcca tccactggtt tctttcattt
     3121 ttccatcctc attacaagct gattctatag ttctacaact agtagtacca acacatatta
     3181 cattatttcc attttctttt gctctattta ttttgtcagc tgcttcttgg ctaaccatat
     3241 aatattctga atgcatcttg tgatttaaaa catcttctac ttttactggt ctaaaagtgc
     3301 ctaaaccaac atgtagagtt acaaaagcta tatctacacc tttttctttt attttactta
     3361 acaattcttc tgtaaagtgt aatcctgctg taggtgctgc tgctgaacca ttatgctttg
     3421 aataaacagt ctgatatctt tctttttcat ctaatctagc agtgatatat ggaggtagtg
     3481 gcatatttcc aagttcatcc aagatttctt caaaaattcc atcataatga aattttataa
     3541 ttcttgaacc ttcatctgat aaatctacaa cttctccaat taactttcca tttccaaaag
     3601 aaaatttagt ccctactttt gctctttttc ctggttttac taaagcctgc caagtatctt
     3661 cctctgttct ttttaataat aaaaactcaa ttttacctcc agtttcaagt ttctctccca
     3721 tcaatctggc tggtataact ctagtattat ttaatactaa acaatcccct ggatttaaat
     3781 attctattat atctctaaat actttatgct caatattgcc tgtctcttta tctaatacca
     3841 tcaatcttga actagctcta tcttctatag gaacttgagc tataagttcc tgtggtaagt
     3901 caaatttaaa atcacttgtt ttcaatgtta aacttcctct cttaaaattt ctccagctac
     3961 aataatatca aaaaaataac ttattttcca caatattacc ataatctatc aagaaattgc
     4021 tatttttaac agacatcata tccacaatat atagatattt ttccacgata tgcacgtatt
     4081 atccacaata tatagataca atacgcacaa attttatcgt atttatccac agtatctaca
     4141 gatataatta ccattttgtg gatatatttg aatttttagc cattttagaa atattttaat
     4201 ttaatgacta ttctatttta taaatacttg cataatctat ttttattttt cattaggata
     4261 tggaattttt aagtgttcat atgctaatgg catggctacc cttcctctag gtcctctatt
     4321 tataaatcct aattgaagca aatatggctc atatacatct tctattgtat ttctatcttc
     4381 tccaatagaa gctgctaatg tatccagacc tactggacct cctctaaatt tttctattat
     4441 agtcataaga agcttctcat ctacaaaatc aagacccaaa ctatcaactc ctaaaagttc
     4501 taaagcatct tttgccacct tatcagttat ttttccatca gctctaactt gtgcaaaatc
     4561 tctaactctt tttagtaatc tatttgctat ccttggagtc cctcttgaac gtttagcaag
     4621 ttctaacgct ccattagact gtatctttgc atctaaaata tttgatgacc taagtactat
     4681 ttttgaaagc tcatcaactg tataataatc cagtttacaa ataacaccaa atctatctct
     4741 aagtggattt gtaagcatac cagctcttgt agtagctcca ataagtgtaa atttaggaag
     4801 gtcaagtctt atgcttcttg cagatggccc ttttcctatt attatatcta ggcaaaaatc
     4861 ctccattgct ggatataaaa cttcttcaac acttctattg attctatgta tctcatctat
     4921 aaataaaaca tcattttcat ttaaatttgt aagtatggct gccaaatctc cagctctttc
     4981 tattgcaggt cctgacgtaa ttcttaaatt tacccccatc tcattggcta ttatacttgc
     5041 tagtgtagtt tttccaagac ctggtggtcc atataaaagc acatggtcta attgctcatt
     5101 tctcgattta gctgcttcta taaatatact aagttgctct ttggattttt cttgccctaa
     5161 ataatcttct aaagtttttg gtcttaaact gttttctata tctatatctt ccaccttcat
     5221 tgtagaagtt attatcctat tttcatcttc aaaaccttgc atttataccc ctcctttcgt
     5281 acttttgaaa atctacttta tatattttca aatttacata aactccttaa atattgtttt
     5341 aaaatctaaa tattataaaa tccaaatatt attttaagga tttactcatt atatatgtaa
     5401 gtgctttctt tataatagct tctgtattca taccatcttt tttacatttt tgaactgcct
     5461 cttttgattc cgccaaagta tagcctagag ctacaagtgc atctacagac tcatcttgag
     5521 atatcaaggt aggtttttgt gataataatg ttggttcaaa ctctatgtta tttttatcta
     5581 ccttatcctt aagttctaag acaattcttt ctgctgtctt cttaccaaca ccaggagctt
     5641 tggataactt tccaatatct tcacttaaaa tatatgcgcc taattgtgct ggtgatgcaa
     5701 atgatagtat tgaaagtgct accttaggtc ctatttttga cacagatgta agtagttcaa
     5761 acatcttaag ttcttctcta cttacaaaac cacaaagact catatcatct tctcttacaa
     5821 ttaatttagt atatattttt gtggcttctc ccacttgtaa attcattatt gtattggatg
     5881 atacatttat tttatatcct atcccattat tttcaacaac tatactgtct atataaattt
     5941 cttctactgt accttttata taactataca tatttaccac ctatattctt tagagttttt
     6001 tcaagtttgt ttgagtgagc atggcaaata gccacagcta aagcatctgc aacatcatct
     6061 ggttttggaa ctttttttag acttaaaaat gaagttacca tctgttgaac ttgtgcttta
     6121 tctgctctcc catatccaac tacaccttgt tttacttgaa gtggagtgta ttcatataca
     6181 ggcttcccat tatgagcaca tgcaagcata gtaacacctc tagcttgtgc aactgttata
     6241 gctgttttta cattcttgtt aaagaataat tcttccattc caacttcatc tatattgtaa
     6301 ttctttacta ttgtatcaat tcctttatat acaagttcta atcttctcga tatattcata
     6361 tgggcaggtg ttgtaactgc tccataatcg attactttaa acttgctatt tttgtattca
     6421 attataccat atccaactat agctatacct gggtcaatcc ccaatattat cattttttaa
     6481 aattccccct aaaaataaaa gaaataccaa aatgtatttc tcataaaaac ttatgttcta
     6541 aatcattata tcataaataa tattaacaaa tcataaaatg ctctatgtaa aatataaaat
     6601 caaataaaaa agttatatca aagcaagatt ttacctcaat ataactttta tatttagtac
     6661 aaataaatta ttgcattttt atatgaatta aatgtgacta caattttaat actaatctat
     6721 ctaatattaa attatttatt attaacttac ttagtattta acttgaaata gtattaattt
     6781 actattaact tgcaaagtct tctaatcttg aataggcctc ttcttcagta tccataacta
     6841 cacctttttt gatttcatct tgaactgttt taggaagtga atctactctt atttttgttt
     6901 tttctactaa tgttttactt ccagtatcat catatttata tagtgctata tttccattat
     6961 cattttcaat agtatattta ccttttatag taacatccat tgcttgtaca tctttattat
     7021 cactatattt agattctacc aatatgattt catttttatc catactcttt atttttttag
     7081 ttggatattc ttttttaaaa tatgatacta tttctgactt tgtcttatct aataatgact
     7141 ctggaactat tcctgccatt gtaagtgttc tcttatagtt ggtttcattt tcgccttcac
     7201 tttgaagcca aacttcacaa tttttattta atccaaaagc ttcttttgac ttttcattca
     7261 tattcgcact agtacttaca acttcaccag tttgaactcc attgtgtttt aatcttgtcc
     7321 ctatataaac tccactactt aatacaaata ctccaactag tattaaggca gatattttcc
     7381 aaggaaataa ttcttttttc ttcttttcaa acattgatta cgcctccttt ttctattatt
     7441 ttctccaagc tgtaagtttt tatacaattt aggtaaattt tagaagaaaa tatatttata
     7501 tttcaaatta ctctaagaat agatattttc taaatggtag ttttctctta atagttatat
     7561 tcttgtaatt atatggacat aatttataat aaataatcgt ttaggaggta ataactttgg
     7621 aaagaataaa ctgtaatgta attaactgtt cacataacaa cagtcatata tgttatgc
//
LOCUS       DAFNNB010000162         7650 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0167, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000162 DAFNNB010000000
VERSION     DAFNNB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0167"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(19..1401)
                     /locus_tag="L3M60_003082"
     CDS             complement(19..1401)
                     /locus_tag="L3M60_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HBN6227716.1"
                     /translation="MSVLKTQEKLKQPKDLYFCCSIFTFVGLAYYGMKLVLLLFLAEQ
                     VSKGGLGLTPAESASMLASFLSWTYFSPVIGGWVSDRFLGPKNCIILGMLMVSIGYFL
                     GYIANSKMDINIMIFLSGLGIGFYKGNLHTMVGNLYTNDDPRKDGAFSITYMATNLGT
                     LFGPLICGLVANEWFAMKSGASISMYGYRYVFLFSSITVLIGLFFFVFGNRKFYNSLN
                     NNHSNERESIRKSLEYKKMLASRPLTLIEKKRIIVILVLAFFTVFFWIAYNQASMSIA
                     LYTKEHINLTVGNFQIPTSWIDSYNGLLCVILGPISALVWVKLSQTKKGDLSVPKKMS
                     LGFILLAIAFLFMIVAVMQTGTDSNSIHKASVFWVVGFLTFQSIGEICFSPVGYGMVN
                     KLSPEKYISLLMGVWFLGKFAANKLSGYTQAIIDQLGMLQVFIVIPSFLLIFGIILLI
                     MNKKLVELSS"
     gene            1659..2006
                     /locus_tag="L3M60_003083"
     CDS             1659..2006
                     /locus_tag="L3M60_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBN6227717.1"
                     /translation="MIRKKSELQVEVKENLNGGIGNVKLENIIQNEELKGKGRLFKRV
                     SLPVGSSIGVHDHTTDFEVYYILKGKGKVFDNGEFVEVNEGDVVYTADGEKHSIENIG
                     EEDLEFVALVLYM"
     gene            2024..3058
                     /locus_tag="L3M60_003084"
     CDS             2024..3058
                     /locus_tag="L3M60_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227718.1"
                     /translation="MTGKIEEIKDKINRFCNSNLNQEYKDISFKILQNLLDNNKEIIH
                     SSRADIWSSAILNIVLEQNLLYNKKHPLHITKKEFSKQIGVSLNTINNKSSLIKDVCS
                     IDFLNQDTIAISNVEFWVKEINSKSKSVDLDLEKKKILYKRYIKLSQDANSDIESIRY
                     MEEAVNIGKSMITKEDKELGFWKGLSTRAYMIALESLARKLESINNLNESKKVLEYLI
                     EINPSDEQGIRYKLLNVLIRLNDRTSINKLFELYKEEKSATWMYSKALYYFKNKSMFL
                     ANDSIKVARGKNKYVGLYLLDWRNAFGREFVTEEEKAEAVYYYDENIVIWNEVKGSMD
                     WLLKKMLEFS"
     gene            complement(3107..4156)
                     /locus_tag="L3M60_003085"
     CDS             complement(3107..4156)
                     /locus_tag="L3M60_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HBN6227719.1"
                     /translation="MEINYKKRNFKSFAKRKNIEISVQRYLIDALSYMALGLFSSLLI
                     GTIFNTLGDNFNISLFTEVISPLAKQVTGPAIAVAIAYGLQAPPLVLFSCALVGACGN
                     ELGGPVGAFVATLFAVEIGKLVSKETKIDILVTPAVTILIGVLVAELIGPAVSAFMTS
                     FGNIIMQATTMQPIIMGALVSALVGIALTLPISSAAICMMLSLGGLAGGAATVGCCCQ
                     MIGFAVMSFRENGVGGLVAQGLGTSMLQVPNIIKNWKIWIPPTVASILLGPLSTTIFK
                     MENIPIGSGMGTCGLVGQFGTVTAMQSVGKGGTSMWVGILLLNFILPAIVTLLISEFM
                     RKKGLIKPEDLKLDV"
     gene            4431..5966
                     /locus_tag="L3M60_003086"
     CDS             4431..5966
                     /locus_tag="L3M60_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227720.1"
                     /translation="MRFIKNKKYRKIAIIISIPILLVAQYYLFKFGILRPMVKGVEIE
                     IVDGEYVKDIDKYVVKLNETVTLSTGEYIKVPSYAKDPNIWFNVLDNSGTLKIDGNKM
                     TALKVGYSSVGIMKNSRVLKKATIKVVDPEVESLDMEINGDLKHVGDSATIESTIEVD
                     YDKFKKSYKPVYKSSNENILKIKGNRVSAVGVGKATIYAKCGNKEVERTFRIEARVAE
                     INMDDIKIEVDQNEKLKPDIITSPPGLEPPKIYYELVDSKLPIERAISLSSNGNIVGL
                     REGKDRVRITCGDKSKIITVTVVKETITNDYIQNLVGSHKIVNNKVIIKLEWDYMKDV
                     FDYDIYLKDKLSGESFKKIKSITINESDLGKSNKVYATIEVDMKGNQNVDFDIYVIGK
                     KDSDTTKPSNVINITNGNGNSNNDNNIKDMKVESISANVDRDNNVIRVNWSSISHKDC
                     TYSIYVKNNTNGDGEFTLYQSDIQGNEHTISIPEGDLNLDIYVVANYNGNNSQNSDVI
                     NVR"
     gene            complement(6113..6463)
                     /locus_tag="L3M60_003087"
                     /pseudo
     CDS             complement(6113..6463)
                     /locus_tag="L3M60_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
     gene            6465..7526
                     /locus_tag="L3M60_003088"
                     /pseudo
     CDS             6465..7526
                     /locus_tag="L3M60_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729078.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
BASE COUNT         2986 a          975 c         1198 g         2491 t
ORIGIN      
        1 attaattttg aaaaattttt aacttgataa ttctactaac tttttattca ttataagtag
       61 tataattcca aatatcaata aaaatgatgg tatcactatg aatacttgta acataccaag
      121 ttggtctata atagcttgag tataaccaga taatttattt gctgcaaatt tacctaaaaa
      181 ccaaacaccc attagtaatg aaatatactt ttcaggcgaa agtttattta ccataccata
      241 tccgactgga gaaaagcaaa tttctcctat actttggaat gttaaaaacc caactaccca
      301 aaatacactt gctttatgaa ttgaattaga gtccgtacct gtttgcataa ctgctactat
      361 cataaataaa aatgctatag ctaatagtat aaaacctaag ctcatttttt tgggtacact
      421 taaatcgcct tttttagttt gagataattt tacccatact agagcagaga taggtccaag
      481 aataacacaa aggagaccat tatatgaatc tatccatgaa gtaggaattt gaaaattgcc
      541 aacagtaaga tttatatgct cttttgtata gagtgcaata gacatagagg cttggttata
      601 tgctatccaa aaaaatactg tgaaaaaagc taataccaat attactatta ttcttttttt
      661 ctcaatcaaa gttaatggtc ttgaagcgag cattttctta tattctagtg attttcttat
      721 agattctctc tcattactat ggttattatt taaagaatta taaaactttc tgtttccaaa
      781 tacaaagaag aataaaccta ttagaaccgt aattgatgaa aataagaata catatctgta
      841 tccatacata cttatagagg ctccactttt catagcaaac cattcattag caactaaacc
      901 acatattaaa ggaccaaata aagtacctag attagtagcc atatatgtaa tagagaaagc
      961 tccatctttt cttgggtcat cattggtata taagtttcca accatggtat gtaagtttcc
     1021 tttataaaaa cctattccta atccagataa aaatatcatg atgtttatat ccatcttact
     1081 atttgctatg tatcctagaa aataaccaat tgaaaccatt agcattccaa gtatgataca
     1141 gtttttggga cctagaaatc tatcactgac ccatccaccg ataactggag agaaataagt
     1201 ccatgataaa aaagatgcaa gcattgatgc ggattctgct ggtgttaacc ctaaaccacc
     1261 tttggagact tgttcagcta aaaataataa taataccaat ttcatgccat agtatgcaag
     1321 tcctacaaag gtaaatattg aacagcagaa atataaatct ttaggttgtt taagtttttc
     1381 ttgcgttttt aaaactgaca tatgtcgaga acctccagat aaatagttta acaaaatttt
     1441 accatactat atgagtatat gtttttttca tagagacgtc aacttatgag ataatttaat
     1501 taagtcgaaa tatatgtaaa aatagtatca tttttgtgta ttcaataata tttttttatg
     1561 ataatatatg ttattattat attaaaatac aaacatattt atggaaagga tatgccaatt
     1621 tagtagttag attgattgtt tggcaggtgg gatgagctat gataagaaag aaatctgaat
     1681 tacaagtaga agtaaaagaa aatttaaatg gtggtatagg aaatgttaag cttgaaaaca
     1741 taatacaaaa tgaagagctt aaaggaaaag gaagactatt taaaagggta tctttaccag
     1801 taggaagttc aattggtgtg catgaccaca caacagattt tgaagtatat tatatactaa
     1861 agggaaaagg caaagtattt gataatgggg aatttgtaga agtaaatgag ggtgatgtag
     1921 tgtatacagc tgatggcgaa aagcattcta tagaaaatat aggagaagaa gatcttgagt
     1981 ttgtagcact agttttatat atgtaaaata tggaggagga gtcatgacag gcaagataga
     2041 agaaattaag gacaagataa atagattttg caactccaat ttaaatcaag aatacaagga
     2101 tataagcttt aaaattttgc aaaacctttt agataataat aaagagataa ttcattcttc
     2161 tagagctgat atatggtctt cagctatact aaatatagtt ttagaacaaa atcttttata
     2221 caataagaag catccactac atataacaaa aaaagagttt tcaaaacaaa taggtgtttc
     2281 tttaaatact ataaataata aatcatcttt aataaaggat gtttgtagta ttgacttttt
     2341 aaatcaagat actatagcta tttctaatgt agaattttgg gtaaaagaga taaattccaa
     2401 atcaaagtct gtagatttag atttagaaaa gaagaaaata ctatataaaa gatatattaa
     2461 gctatcacaa gatgcaaata gtgatattga atcaattaga tatatggagg aagctgtaaa
     2521 tataggtaaa agtatgatta ctaaagaaga taaagaactt ggattttgga aaggtttatc
     2581 taccagagcc tatatgatag cacttgaatc ccttgctagg aagttagaaa gtattaataa
     2641 tctaaatgaa tccaaaaaag tattggaata tttaatagaa attaatccta gtgatgagca
     2701 gggaataaga tataaattat taaatgtatt aattagatta aacgatagaa cctcaataaa
     2761 taaattattt gaactttata aagaagaaaa atctgctaca tggatgtaca gtaaagctct
     2821 ttattacttt aaaaataaaa gtatgttttt ggcaaatgat tctattaagg ttgctagagg
     2881 taaaaataag tatgttggat tatatttgtt agattggcga aatgcttttg gtagagaatt
     2941 tgttacagaa gaagaaaagg cagaagctgt atattactat gatgaaaaca tagtgatttg
     3001 gaatgaagtt aaaggttcta tggattggct attaaaaaaa atgttggaat tttcataaaa
     3061 aaaactactc cttactttta tataagtaaa aggagtagtt tttaatttac acatctagtt
     3121 ttaaatcttc aggttttatt agaccttttt ttctcataaa ttctgaaatc aacaaagtta
     3181 caatagcagg aagtataaaa ttaagtaaaa gtataccaac ccacatagat gtacctccct
     3241 ttccaacact ttgcatagca gtcactgttc caaattgtcc aaccaatcca caagtaccca
     3301 taccagagcc gataggaata ttctccattt tgaatattgt tgtagataat ggtccaagta
     3361 atattgaagc tacagtaggt ggtatccaaa ttttccaatt ttttattata ttaggtactt
     3421 gtaacataga tgtaccaaga ccttgtgcca caaggccacc aactccattt tctctaaagc
     3481 tcataacagc aaagcctatc atttgacaac aacaacctac agttgctgcc ccaccagcaa
     3541 gaccacccaa acttaacatc atacaaatag ctgcactact tataggtaat gtaagagcta
     3601 ttccgacaag tgcagagact aaagctccca ttattatagg ttgcatagtt gtagcttgca
     3661 ttattatatt tccaaatgaa gtcataaaag cagaaacagc tggaccaata agctcagcta
     3721 caagtactcc aattagtatt gttacagcag gtgtaaccag tatatctatt tttgtttctt
     3781 ttgaaactaa ttttccaatc tctacagcaa aaagtgtagc aacaaatgct ccaacaggtc
     3841 ctcctaattc attaccacaa gctccaacaa gtgcacaaga aaatagtaca agtgggggtg
     3901 cttgaagtcc atatgcaata gccactgcta tagctggacc ggtaacctgt ttagcaagag
     3961 ggcttataac ctcagtaaaa agagaaatat tgaaattatc tcctaatgta ttaaaaattg
     4021 ttccaattaa aagggaagaa aaaagaccga gtgccatata acttagtgca tcaataagat
     4081 acctttgaac agaaatttca atgttttttc ttttagcaaa tgatttgaaa tttctttttt
     4141 tataattgat ttccaattta aaatcctcct catataatat aagtataatt tctttttaat
     4201 taattatata acttttcagc tgacaagaca aatgttattt cactaaaatt agactattgc
     4261 aatagtaaga aattaacatt aattttagtt ttaaatttta aatttatggt ttataattag
     4321 tatagggata ttctgaaata taataatttt ccagtttatc ccaatattta acttaggtag
     4381 attgcttaca aaacaaatga ttatatatta agctaaaagg aagtggatag atgagattta
     4441 taaaaaataa gaaatacaga aaaattgcta taattatatc tattcctata ttgcttgttg
     4501 cgcagtacta cctttttaaa tttggaatac ttagaccaat ggtaaaggga gtagaaattg
     4561 aaattgtaga tggggaatat gttaaagata tagataagta tgtagtaaaa ttaaatgaaa
     4621 cagttacttt atccactggt gaatatatta aggttccttc gtatgctaaa gatccaaata
     4681 tttggtttaa tgtattggat aattcaggaa ctttaaaaat agatggaaat aaaatgacag
     4741 cattaaaggt tgggtattct tctgtgggta tcatgaaaaa ttcaagagta ttaaaaaagg
     4801 ctactataaa agtagtagac ccagaggtag agtcactaga tatggagatt aatggagatt
     4861 taaaacatgt tggtgatagt gctactatag aaagcacaat agaggttgat tatgacaagt
     4921 ttaaaaaatc atataagcca gtttataaga gtagtaatga aaatatttta aaaatcaaag
     4981 gaaatagagt aagtgcagtt ggagttggaa aggctacaat atatgctaag tgtggaaata
     5041 aagaagttga gagaacattt aggattgaag ctagagttgc tgaaataaat atggatgata
     5101 taaaaataga agttgaccaa aatgaaaaat tgaaaccaga tataataact tctccacctg
     5161 gtttagaacc accaaaaata tattatgagt tagtagattc aaaattacct atagaaagag
     5221 caataagttt aagtagtaat ggaaatattg tagggcttag agaaggcaaa gatagggtaa
     5281 gaataacttg tggagataaa tctaaaataa taacagtaac agtagtcaaa gaaactataa
     5341 cgaatgacta tatacaaaat ttggttggct cacataaaat agtaaataat aaagttatta
     5401 ttaaacttga atgggattat atgaaagatg tctttgatta tgatatatat cttaaagata
     5461 aattgtcagg agaatcattt aagaaaatta aatctataac tataaatgaa agtgatttag
     5521 gtaagtctaa caaagtatat gcaactatag aagtagatat gaaggggaat caaaacgtag
     5581 attttgatat ctatgtcata gggaaaaagg attcagatac aactaagcca agtaatgtaa
     5641 taaatattac taatgggaat ggaaattcaa ataatgataa taacattaaa gacatgaaag
     5701 tagaaagtat atcagctaat gttgatagag ataataatgt tattagagtc aactggagta
     5761 gtatatctca taaggattgt acttacagca tatatgtaaa aaataatact aatggagatg
     5821 gagaatttac tctatatcaa agtgatatac aggggaatga gcatactata agtattcctg
     5881 aaggagactt aaatctagat atttatgttg ttgcaaatta taatgggaat aactctcaaa
     5941 atagtgatgt tataaatgta agatagattt gtatacaaat gagtattaag tttattgaaa
     6001 aattaaatta agaaaaagga tagtgtgata gcaatcatac tatccttttt tgttaaaata
     6061 tagaggtttc aaatttttag ttaaaaaaat ttttttatta attgatattg tattaaattg
     6121 aacttagtgc tgcttctatt gcatgtatta ttgttgtatc aaatagtgga atagaagtat
     6181 cattttgttt aattaaaaga cctgtttctg tacaacctaa tataacaccc tctgcacctt
     6241 gcttgccaag cttatctata atagataaga aagctttctt tgaagattca gatacaattc
     6301 caacacaaag ttcattaaat attgtattat ttactattat acggtcttct tcatctggaa
     6361 ttaaaacatc aattccatta ttaataatta tgtttttata aaaatcttgt tccattgtat
     6421 atttagtacc taataaagct acttttttta tttctttttc ctttgatgta aatgtaagta
     6481 atacattatt atatcaaaca gaatcaaaag acataattga actaggaaca gttcatcctc
     6541 catatgagca aaatacatat attattgatt ttattaaaag tatactaaat caaccagaaa
     6601 tagataaatt tttacaatat atgtctccat ctggtacgta catgcaaaaa aaatcaattg
     6661 caaaatggat agagagaaat aatatataca ctagtgaaga aaatatctta ttatctacag
     6721 gagggcaaaa tgctatatgt gcaaccttac taggattatt taaagcgggt gataggattg
     6781 ctacagattc tttgagtttt ctggaattaa atcaatagct aaaataatag gtattcaatt
     6841 ggttccaatt cctcaagata ataatgaaat gtcgattgaa catcttgaaa gttattgtaa
     6901 aaatgaaaat ataaaaggca tatatcttat accagattat cataatccaa cgacacattc
     6961 tatgtctgat ttatcttgta aaaagatagc aaaacaataa atataataat cattgaagat
     7021 gccataaaca gtatttttag agatagtaca caaactccta tgttttcttt ggccagtgat
     7081 aatactatat atattttttg tacatctaag tttttatgtg ctggtcttag aatagcattt
     7141 gtagttgccc caaaacgata tatagaaaac ttagaagatg cactatataa tatgaatcta
     7201 atggtttctc catttacggc agaaatagta caccgtttac tatattctcc tattttagat
     7261 aagattataa aagagaaaaa agatacaatt atagaaggaa ataaagttgc tgataaagtt
     7321 ttgtcagatt ataatttgat gaattggagg gaaagtcatt tgaaatatct gcaaaaaatc
     7381 taggtgttca agtgtattgt gcagaacgat tttcagtagg caactctaca gtaccaaaag
     7441 caatccgtat ttgcataaca gctccaaaag atgtagaaga attagaaaaa gggctcaata
     7501 taataaagtc acttcttaat agctaaattt attttactat gatttaatta aataaataaa
     7561 aaggcggaga aatttattat aataaacctc cgcttttatt atttaatgaa atactttact
     7621 ttctattaaa aatttaattt taataatctt
//
LOCUS       DAFNNB010000163         7364 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0168, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000163 DAFNNB010000000
VERSION     DAFNNB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7364
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0168"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            62..751
                     /locus_tag="L3M60_003089"
     CDS             62..751
                     /locus_tag="L3M60_003089"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBN6227721.1"
                     /translation="MRSENLVKMAELKNTEKLEKFNRHKGKIKIGILGGTFDPIHYAH
                     LATAEFIRDKYDIDKIIFIPSGNPPHKLCITTDKYDRYNMTLLATESNEDFLVSKVEI
                     ERKKRTYTIDTLKYLKKKYKNADIYFITGADAICSVEEWKDVKKNFELATFIAATRPG
                     ISLLRSQETIEKLTKKYNADIITVYVPSLDISSTYIREQLNEGKSIRYLVPENVENYL
                     YENKLYQYGDD"
     gene            752..1324
                     /gene="yqeK"
                     /locus_tag="L3M60_003090"
     CDS             752..1324
                     /gene="yqeK"
                     /locus_tag="L3M60_003090"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HBN6227722.1"
                     /translation="MNLENINQRLNQMLPVGRLSHSKNVAKCAEKLCEIYGCDKEKAY
                     LAGMIHDCAKYLSDKEIEDYVNKYEIYLDPIEDGNRSLSHSVIGAYICEYEFEVEDED
                     IINAIKYHTTGREDMSLLEKIIYIADLIEEGRKFPVVDALRELAYGGKLDEALLTSFN
                     NTLMFVINKKEEIHPRTVMARNYLIKEKLL"
     gene            1441..1791
                     /gene="rsfS"
                     /locus_tag="L3M60_003091"
     CDS             1441..1791
                     /gene="rsfS"
                     /locus_tag="L3M60_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBN6227723.1"
                     /translation="MTVEQMTKIAYDAIEDKLGQDTVIINIGKVSSLCDYFIITTASS
                     QRQVKAIADNVEDELAKLGLEPRGKEGQGTQTWVLLDYGDIMVHVFNEENRGFYNLEK
                     LWKDAPYIDIDTLA"
     gene            2145..4565
                     /locus_tag="L3M60_003092"
     CDS             2145..4565
                     /locus_tag="L3M60_003092"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBN6227724.1"
                     /translation="MSVYNFKEVESKWQKIWKDNNQYKMDTAQTEKPNYYTLEMFPYP
                     SGKIHMGHVRNYSIGDVVARFKKMEGYNVLHPMGWDSFGLPAENAAIKHGIHPHKWTM
                     ENIEEMKEQLNLLGISYDWDKEVATSTPEYYRFTQEIFLKFLEHGLAYKKKSYVNWCP
                     SCETVLANEQVVQGACERCKATVLKKDLEQWYFKTTEFAEELLNDLDTLDGWPEKVKT
                     MQKNWIGKSTGADLVFDIDGTDKSMTVFTTRPDTTYGVTYMVLAPEHELVKELVAGTE
                     YEADVEAFVQKMHTMTEIERTAADVEKEGMFIGRYVINPLNGKKVPLWIANYVLVEYG
                     TGAIMAVPAHDERDRDFAEKYNLDIIDVITEDNKMINSEEFNGLDASEGFEGIIDKLE
                     KEGRGKRTINYRLRDWLVSRQRYWGCPIPVVYCDECGIVPVKKEDLPVLLPTDVEFTG
                     KGESPLTTSKQFMSTTCPHCGKPARREVDTMDTFVDSSWYFLRYVDSNNENEPFSKEL
                     VNRWHPVDQYIGGVEHAIMHLLYARWFVKAFKSMGMVDFNEPFKNLLTQGMVLMDGSK
                     MSKSKGNTVSPMDIIDEYGADTARLFVLFAAPPERDLDWSEQGVDGCFRFLNRVYRLV
                     DELADVVKKDVEFGELNSQDKDMRYTIHSTLKKVTADLSEKFGFNTAISALMELINDM
                     YKYKELDNINEAVIKEGVQTIVTIIAPFAPHLGEELWTMIGKEGSVFDIDWPKYDEKA
                     LVKDEIEVVVQVNGKVRGKLTVNSNISKDEMEKVALEDEKIKGLVEGKTIVKVVAVPK
                     KLVNIVVK"
     gene            4906..5967
                     /locus_tag="L3M60_003093"
     CDS             4906..5967
                     /locus_tag="L3M60_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227725.1"
                     /translation="MYQRIIFPMLKREVNIPGNLNYQYKYNGVKESKSVKTSWEFKDE
                     DKRKFRLMCLPNSKTVSAIINDLIYFHSTEEYKLQFIGMRMNVLVDIWAHMYFIGKPE
                     WYINDIKEFIAEESKYIAENKWTKSEEYRNGWHAINVSGTLDACGYESISYLGYGRMG
                     NVPDYGYLNYRYVPNWSCTDDKVKIQKNNQDNFFKAFCQMFYALKCIKNGNDFNINQY
                     DNLTAQQKTEVTKVIATRKTDQSDAWEKVIKVLGYSSLEKFDKNKWKNEFEKFSNKKK
                     INYYYFNLVAKKHIDYVNNFLEENRLKLDGMCSEYCELKVFDDTQFAKNKNRVIKIVL
                     RGDFIVDAIQLVYDGKCKT"
     gene            complement(6696..7256)
                     /locus_tag="L3M60_003094"
     CDS             complement(6696..7256)
                     /locus_tag="L3M60_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227726.1"
                     /translation="MKLRNPKIKKLLSVALLAPLVGIPIGQTANAMEISNDIPSYIEN
                     NPITIEINDSQLPKPGTVTYIDMNKLIKNLKENGIDTTIIENELYYRKAGTNKIVWTK
                     MGFDLYLSKGVALTLAGAGTGVAGILAGAIPGIGQYLAAAIVGAITMHLGSNITSGKI
                     FKFKKMRYNKGGTYVTKYYLQSVRNQ"
BASE COUNT         2935 a          819 c         1326 g         2284 t
ORIGIN      
        1 aagctttaag aaataaaaaa taatggtaca atagtattat aacattaaaa ggagacaaaa
       61 tatgagaagc gaaaatctag ttaagatggc agagttgaaa aatactgaaa aactagagaa
      121 atttaatcgc cataaaggca aaatcaaaat aggtatattg ggaggtacat ttgaccctat
      181 acactatgct catttagcaa cagcagaatt tattagagac aagtatgaca ttgacaaaat
      241 aatctttata ccatcaggaa atccaccaca taaattatgt atcactacag ataagtatga
      301 tagatataat atgacacttc tagcaactga aagcaacgaa gattttttag tttcaaaagt
      361 agaaatcgaa agaaagaaaa ggacttatac aatagataca ttaaaatact taaagaaaaa
      421 atacaaaaat gcagacattt attttataac aggtgcagat gcgatttgta gtgttgaaga
      481 gtggaaagat gtaaagaaaa actttgagtt agctactttt atagcagcaa ctagaccagg
      541 aattagtttg ttaaggtcac aagagactat agaaaaacta actaaaaagt ataatgctga
      601 cattataaca gtttatgttc catcactgga tatatcgtcc acatatataa gggaacagct
      661 aaatgaaggc aagtcaatac gttacttagt acccgaaaat gtagaaaatt atttatatga
      721 aaataaacta tatcaatatg gagatgatta aatgaactta gaaaatatta accagaggct
      781 aaatcaaatg ctaccagtgg ggaggcttag ccattcaaaa aatgttgcaa aatgtgctga
      841 aaaattatgt gagatttatg gatgtgacaa agagaaagct tacttagcag gaatgattca
      901 tgattgtgca aagtatttaa gtgacaagga aatagaggat tatgtaaata agtatgaaat
      961 atatttagac ccaatagagg atggaaaccg ttccttgtct catagtgtaa ttggggcata
     1021 catatgcgag tatgagtttg aagtagaaga tgaagatatt ataaatgcaa tcaaatatca
     1081 tacaacaggg agagaagata tgtctctttt agaaaaaata atttacatag ctgatttgat
     1141 tgaagaagga agaaaattcc cagttgtaga tgcattaaga gaattagcat atggaggtaa
     1201 acttgatgaa gcacttttaa cttcttttaa taatacttta atgtttgtca tcaataaaaa
     1261 agaagagata catccaagaa cagttatggc aagaaactat ctaattaaag aaaagttatt
     1321 ataaaaaacc tatataaagg taaacatatt aataaattta aagttgacta tgttacaaaa
     1381 tcgtattaat atgttacaat aactaagtgt attttaatta ggaaagtgag gaatttttat
     1441 atgacagtgg aacaaatgac aaaaatagca tatgatgcca tagaagataa acttggtcaa
     1501 gatacagtta taataaatat aggaaaagtt tctagtttgt gtgattattt tattattact
     1561 acagcatctt ctcaaagaca ggtaaaagct attgctgaca atgtggaaga tgaattggca
     1621 aaactaggat tagagccaag aggtaaggaa ggacaaggga ctcaaacttg ggtgctcctt
     1681 gattatggag acataatggt tcatgtattt aatgaggaaa atagagggtt ttataattta
     1741 gaaaaattat ggaaagatgc accttatata gatattgaca cattagcata aacatgataa
     1801 tatatgttat tataattaga aaatttcaaa aattatatta aattgaatag actagagcag
     1861 taaaagatta tattttttca gagagttagt ggaaggtgag aactaatatt atataacttt
     1921 gaacttacta gattaaaatc tattctccat ggctggatta aaagctatct aagagggtct
     1981 atgtatatag tagactaatt agggtggtac cgcgaagatt tatcctcgtc cctaaacgta
     2041 agtttagtga cgaggatttt ttattttcaa agaattattt ggaaattata agtaaataat
     2101 actaaacaag gtagttatac acaactaaaa ataggaggta aaatatgagc gtatataatt
     2161 ttaaagaagt agaatcgaaa tggcaaaaaa tttggaaaga caataaccag tataaaatgg
     2221 atactgcaca aactgaaaaa cctaattatt atacattaga aatgttccct tatccatctg
     2281 gaaaaataca catgggacat gttagaaatt attctatagg tgatgttgtc gcaagattca
     2341 aaaaaatgga aggttataat gtacttcatc caatgggatg ggattctttt ggattaccag
     2401 ctgaaaatgc agctataaaa catggaatac atccacataa atggacaatg gaaaatatag
     2461 aagaaatgaa agagcaatta aatttattag gtattagtta cgattgggac aaagaagttg
     2521 caacttcaac tccagaatac tatagattta cacaagaaat atttttgaag tttttagagc
     2581 atgggcttgc atataagaaa aaatcatatg ttaactggtg tccttcttgt gagactgttc
     2641 ttgcaaatga gcaagttgtt caaggagcat gtgagagatg taaagcaaca gtattaaaga
     2701 aagacttaga acaatggtat tttaagacta ctgaatttgc agaagaattg cttaatgact
     2761 tagatacatt agatggatgg ccagaaaagg ttaaaacaat gcaaaaaaat tggataggta
     2821 aaagtacagg agcagattta gtttttgata tagatggaac tgataaatct atgacagtat
     2881 ttacaactag accagataca acttatggag ttacttatat ggtacttgct ccagagcatg
     2941 aattagttaa agagttggtt gcaggtactg aatatgaagc agatgttgaa gcttttgttc
     3001 aaaaaatgca tactatgaca gaaatagaaa gaacagcagc tgatgtagaa aaagaaggaa
     3061 tgtttatagg tagatatgtt ataaatcctt taaatggtaa gaaagttcct ttatggatag
     3121 ctaattatgt attagttgag tatggaacag gtgccataat ggcagttcca gcgcatgatg
     3181 aaagagatag agattttgct gaaaaataca atttagatat aattgatgta ataacagaag
     3241 ataacaaaat gataaattcg gaagagttta atggattaga tgcttcagaa ggatttgaag
     3301 gaataataga taagttagaa aaagaaggta gaggaaagag aacaattaat tatagattaa
     3361 gagactggtt agtttcaaga caaagatact ggggttgtcc tatacctgta gtttattgtg
     3421 atgaatgtgg aatagttcca gttaaaaagg aagatttgcc agttctttta ccaacagatg
     3481 ttgaatttac tggaaaaggt gaatctccac ttactacttc aaagcaattt atgtcaacaa
     3541 cttgtcctca ctgtggaaaa ccagctagaa gagaagttga tactatggat acatttgtag
     3601 attcttcttg gtatttctta agatatgtgg atagtaataa tgaaaatgaa ccatttagca
     3661 aagaattagt taatagatgg catccagtag accaatatat aggtggagta gaacatgcta
     3721 taatgcactt actttatgca agatggtttg taaaggcatt taagagtatg gggatggtag
     3781 acttcaatga gccatttaaa aacttactta ctcaaggaat ggttcttatg gatggttcta
     3841 aaatgagtaa atctaaaggg aacacagtat ctcctatgga tataatagat gagtatgggg
     3901 cagatactgc aagacttttc gtattatttg cagcaccacc agaaagagat ttagattggt
     3961 cagagcaagg tgttgatgga tgctttagat tcttaaatag agtttataga ttggttgacg
     4021 agttggcaga cgtggttaaa aaagatgtag aatttggaga attaaattct caagataaag
     4081 atatgagata tactatacac tctacactta aaaaagttac tgctgattta agtgaaaaat
     4141 ttggatttaa tactgctata tcagctctta tggaattaat aaatgatatg tataaatata
     4201 aagagttaga taatataaac gaagcagtta taaaagaagg tgtacaaact atagtaacta
     4261 taattgcacc atttgcacct catttaggtg aagaactatg gactatgata ggtaaagaag
     4321 gaagtgtatt tgacatagac tggcctaaat atgacgaaaa agctctagta aaagatgaaa
     4381 tagaggttgt tgtacaagtt aatggaaaag ttagaggaaa attaactgtt aactcaaata
     4441 tatctaaaga tgagatggaa aaggtagctt tagaagatga aaaaataaaa ggattagttg
     4501 aaggtaaaac tatagttaaa gttgttgctg tacctaagaa gttagttaat atagttgtta
     4561 aataacttaa aaatataaat ttataaaata aaggaggctg tctcaaaata gagataagcc
     4621 ttttttcatt gttgaaatta taaaaataga tttgatttca tacaagaaaa gttcctaatg
     4681 aactaaaaag ttcattctga gacaccctca ttttcatctt gaaaattatt acaattaatg
     4741 ttaaatatcg tgaatacatt gagagagtat ttgctgtgac ttctgtagat attaaagtat
     4801 aagaagaaat aaaagaatat taaagtaact agaagaatat aaagcgtagg taaaatatgt
     4861 aagaattata tgctattgta acttacaatt tatagagtgg agtttatgta tcaaagaatt
     4921 atcttcccaa tgctaaaaag ggaggtgaat atacctggaa atttaaacta tcaatataaa
     4981 tataatggag ttaaagagag taaaagtgta aaaacaagct gggaatttaa agatgaagat
     5041 aagagaaagt ttaggttgat gtgtttacca aatagtaaaa ctgtttctgc cattataaat
     5101 gaccttattt attttcatag tactgaagaa tataagttac aatttattgg aatgagaatg
     5161 aatgtacttg tagatatctg ggcacacatg tattttattg gaaaaccaga atggtatatt
     5221 aatgacatta aagaatttat agcggaagaa agtaaataca tagcggaaaa taaatggact
     5281 aaatcagaag aatatcgtaa tggttggcat gctattaatg tttctggtac tctcgatgct
     5341 tgtggatatg agagcatctc atatcttgga tatggaagaa tggggaatgt accagactat
     5401 ggatatctta attacagata tgttccaaat tggagttgta ctgatgataa agtgaagata
     5461 caaaaaaata atcaagataa tttctttaaa gcattttgcc aaatgtttta tgcattaaaa
     5521 tgtattaaaa atggaaatga ttttaatata aatcaatatg ataatctaac agctcaacaa
     5581 aaaacagaag ttacaaaagt aatagcaaca agaaaaactg accaatcaga tgcatgggaa
     5641 aaagtaataa aagttttagg atatagttct ttagaaaaat ttgataaaaa taaatggaaa
     5701 aatgaatttg aaaaattttc taataagaaa aaaataaact attattattt taatttagta
     5761 gccaagaagc atatagatta tgtaaataat tttttagaag aaaatagatt gaaactagat
     5821 ggaatgtgta gtgaatattg tgaattaaag gtttttgatg atacccaatt tgctaaaaat
     5881 aaaaatagag taataaaaat tgtacttaga ggagatttta tagttgatgc tatacagctt
     5941 gtatatgatg gaaaatgtaa aacttaacta catggagaac cttttggagg tatcatagtg
     6001 gatatggatt tagatgcgga tgattatata attaaaataa gtggctctat agggtggttt
     6061 acctatacca gctagaacaa taggtaaaat aactttttta actaaaaaag gaaaaactat
     6121 agcaacagga aaagaacgag catttaagaa atatggaaac tttactctag agccagaacc
     6181 aggtaaacaa atatttgccc ttcagggttc ctactcaata tataaattta gttcatagta
     6241 tagcaaaaga tatttagatt ctctagggat tgcttgtatg aaataattat ataaaaaaat
     6301 atttaaaatt caaattagaa gtttttgttt aatagatata ttagaaggta gcaactagtt
     6361 agttgttact ttccttttta atttaatcag tttttacagc gtttataata taacaaccaa
     6421 ttcatatatt agatttactt ttccgaataa aagtatggca gtataacaag tatctaaaat
     6481 attacaaata ttaaatgaga aaaagatggt aagttaactc caccatctta ttttttatct
     6541 attaatgaat gatgctacaa aagtcagtat aaaaaatgaa acaaacaata taatgaactt
     6601 gtgtaattta cttagttgaa acactttatt agaaacgaat aaaattaatg ctgatacaat
     6661 tgcaaaaagt agatatttta taatcatttt atcacctatt gatttcttac agattgtaag
     6721 tagtatttgg ttacataagt cccaccttta ttataacgca tttttttaaa tttaaagatt
     6781 tttccagatg ttatatttga cccaagatgc attgtaattg ctccaactat agcagcggca
     6841 agatactgtc ctataccagg aatagcacca gccaaaatac ctgcaacccc tgtaccagca
     6901 ccagctaaag ttaatgcaac tcctttagaa agatataaat caaagcccat cttagtccaa
     6961 actattttat ttgttccagc ttttctataa tataattcat tttcaataat agtagtatca
     7021 ataccgtttt ccttaaggtt tttaattagt ttattcatat ctatgtatgt aactgtgcct
     7081 ggtttaggta attgagagtc attgatttct attgttattg gattattttc tatataactt
     7141 gggatgtcat ttgaaatttc cattgcattg gctgtttgcc cgataggtat acctacaaga
     7201 ggtgctaata atgctactga taacaatttc ttaatctttg gatttcttag tttcataaaa
     7261 tacctccatt ttaaattagt tatatatcaa agatatatat tattattgta gaatatagtt
     7321 attaataatt aactatatta aatattaata aaaaatatag taaa
//
LOCUS       DAFNNB010000164         7090 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0169, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000164 DAFNNB010000000
VERSION     DAFNNB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7090
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0169"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..1539)
                     /locus_tag="L3M60_003095"
     CDS             complement(<1..1539)
                     /locus_tag="L3M60_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HBN6227727.1"
                     /translation="MKAFKKHFLILSLIFLLIISCFNNILCFADESENSKKIKVGYCD
                     DYGIISSSKEHSYSGYGYDYLREISKYTRWEYEFIKGSWEECLDRLEKGEIDLLGPLQ
                     KDDERNKIFNFPKLSSGYEYSALYTDDSNNNMFYEDINSFNGMRVAVLRGNFHNIAFE
                     KYREENNFSVEYIYCNSIDELIESVNEKKADAFVCGSIIDAKGMKIVSKFSVEPFYFA
                     TAKDKPNLAKELDYALKELKINDMYYELELYKKYFKREVNNSIAFTRQEINFIKANPK
                     LTMVYDSEWSPVEYYDKKSNSFKGISSDLMSIISKKCGIKFEYIKTKNYNESLDYIKS
                     GKATMICGSINENDKAKRFNMKLTNPYINIPMIMVGKLDTNLNNDLNIALTSSYKSID
                     SYIEKSFENAKTTSYNSVESCLNAINEGKANLMVLSSYQFDELLRKGKSENLSVISVL
                     DTSYGMRIGVSNKTNPILVSILNKSIDKITEEELSDCIYSNTIDKPYKVPFGVIFKEY
                     SIQII"
     gene            complement(1865..2473)
                     /locus_tag="L3M60_003096"
     CDS             complement(1865..2473)
                     /locus_tag="L3M60_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBN6227728.1"
                     /translation="MVDNLIKYIHKSDVAIVVLHEIYGINSFIEDVCQKYYKYGYDVF
                     CPELLGEKRVFPYSKVQEAYDFFINNVGFDIYKKVEKLINDLKSEYKYVFIAGYSVGA
                     TIAWRCSEDSLCDGIICCYGSRIRDYMDVIPKCPTLLVFAKYDSFDVNSISCQLSRKQ
                     NIQIEILDANHGFIDIYSKNYSDIQTKAFTVKEKSFLSKCLV"
     gene            complement(2733..3278)
                     /locus_tag="L3M60_003097"
     CDS             complement(2733..3278)
                     /locus_tag="L3M60_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH peroxidase"
                     /protein_id="HBN6227729.1"
                     /translation="MKKFVCTVCGYIHEGDAAPAQCPVCKVGADKFEEMKGEKNWADE
                     HRIGVAQGVDEEIIEGLRANFVGECTEVGMYLAMSRQADREGYPEVGEAYKRIALEEA
                     EHAAKFAELLGEVVVADTKENLRVRVDAEYGATDGKLKLAKKAKELGLDAIHDTVHEM
                     CKDEARHGKAFLGLLNRYFGK"
     gene            complement(3396..5219)
                     /locus_tag="L3M60_003098"
     CDS             complement(3396..5219)
                     /locus_tag="L3M60_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227730.1"
                     /translation="MNLSTGNQKVSRLKKKEKPKDFKKTFKRIFMYFKKDKKITRFIF
                     LLVIIDSIISTVIPYTIGKVVDIIDIDTYSKSIFLKGIIILLSFYIIEFSVKLSKGIC
                     TARLSQSIVKEMRSNLFLKFKSLPITFFDKSSTGDIMSRVTNDVDNISTGISNSLVQL
                     ITGILNILMTFIMMFVLSPILTFCSVILIPTVMFVSNFIAKRTRVFFKQQQVELGKLN
                     AHIEEMISNINVVKSFNYEIKSVNDFKEINDRLLKISLKSQIYTSLLMPIMNVISNIG
                     FATICIIGGVLASKDIITIGVIASFLSYVRQFTRPLNELANLFNTLLSAIAGCERVFE
                     IIDEKEEQELDNMFKSELKDNKDGNSNSHFVKGEIELKNVSFSYDSKKKILDNINLKI
                     KAGTSNAIIGETGSGKTTIINLITNFYHIDEGSIFLDSKDIYSIDRNYLRTCFGVVPQ
                     EGYIFNDTVMENIRYGNLEATDEEVIYASKIANSHKFIVKMREGYSTVLSDRGEDLSE
                     GQKQLISIARAVLKNAPILILDEATSNIDIATEAKIQEAINNLTYGKTSIIIAHRLST
                     ISNCDNIIVLENGRIVESGNHKELIKLKGHYYRNILLTQGK"
     gene            complement(5232..7007)
                     /locus_tag="L3M60_003099"
     CDS             complement(5232..7007)
                     /locus_tag="L3M60_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227731.1"
                     /translation="MRYFKKYIKKYIVLFLTAVFFLTLEAFCDLAQPTIMAKIIDIGV
                     SNKNLDYILSTGVIMIGITFLGAIFAIIRSVISARVSQHFSYYLRQDVFEKINTYSLK
                     QIDKFGRASLITRITNDINQMQQFVNGMMRVFIKSPIMCIGSLIIAIKLNLKMSIIIL
                     IAVLIIFIIILLNMKVGYRLFKNVQESTDKVNSKLRQFLSGIKVVKLFCRYDYENKSF
                     EELNYELFKNSRKATTMMSFFRPTITIIINVSIIIILLIGRYLIYSGEIKIGIIVAYV
                     NYMTRILTSLIMISHVFNVFVRAKASYERISEVLIDEDEEVIERDKSSDIGEKDLKFA
                     NCDIIFENVDFAYNKNTGEKILKNISFKIEEGQNIGVIGQTGSGKTSLVNLIQKLYII
                     DKGDIKIGGHSIYSISEESLRERIGVVFQNSSVFGKSILENVVMGNRNIRKEDVIKSC
                     EIANANQFITKLENSYDEVLFQRGKNLSGGQRQRITIARAIAKKPQILILDDCFSAMD
                     INTETIIRKNIKDHISNLRENEGKKCTVITISQKISSIIDCDKILVLDEGEVVGFATH
                     EDLLVSSPIYKKIFTLQNLVSEDVN"
BASE COUNT         2395 a         1214 c          627 g         2854 t
ORIGIN      
        1 aataatttgt attgaatatt ctttaaaaat cacaccaaat ggtactttat aaggtttgtc
       61 aatagtatta gaatatatgc aatcacttaa ttcttcttca gtaattttat ctattgactt
      121 atttaaaata gacactaata taggattagt tttatttgat acccctatac gcatcccata
      181 acttgtgtct aaaacagata taacagataa attctcactc tttccttttc ttaaaagttc
      241 atcaaactga taagaactaa gtaccattaa attagcttta ccttcattta tagcgtttaa
      301 acaactttct acactattat aagatgttgt cttagcattt tcaaaactct tttctatata
      361 agaatcaata gacttatagc tactagttaa agcaatattt aaatcattat tgagattggt
      421 atccaatttt cctaccataa tcattggaat atttatgtat ggatttgtca atttcatatt
      481 aaatcgcttt gccttatcat tttcattgat tgaaccacat atcatagttg cctttccact
      541 ttttatgtaa tcaaggctct cgttataatt ttttgtttta atatattcaa attttatgcc
      601 acattttttt gatataattg acattaaatc tgaacttatg cctttaaatg aattactttt
      661 cttatcataa tactctacag gagaccactc tgaatcatac accatagtaa gttttggatt
      721 tgctttaata aaatttattt cttgtcgagt aaatgctatg gaattgttaa cctctctttt
      781 gaaatatttt ttatataatt ccagctcata atacatatca tttattttta attcttttaa
      841 agcataatct aattcttttg ccaaattagg tttatctttt gcagtagcaa aataaaaagg
      901 ttctactgaa aatttactaa ctattttcat tcctttagca tctataatac ttccacaaac
      961 aaatgcatct gctttttttt cattgacaga ctcaatcaat tcatcaatag aattacagta
     1021 tatatattca actgaaaaat tattttcttc tctatacttc tcaaatgcaa tattatgaaa
     1081 gtttcctcta agtacagcca ctctcattcc attaaaggaa tttatatctt cataaaacat
     1141 attattgtta ctatcatctg tatataatgc actatattca tatcctgaac ttagtttagg
     1201 aaaattaaat attttatttc tttcatcatc cttctgtaaa ggtccaagta aatctatttc
     1261 acctttttct aatctatcta aacattcttc ccaagacccc tttataaatt catactccca
     1321 tcttgtatat tttgagattt ctcttagata atcataacca tatcccgaat aagaatgctc
     1381 ttttgatgag gatattattc catagtcatc acagtatcca acttttattt tttttgaatt
     1441 ttcagactca tcagcaaagc atagtatatt attaaaacat gatataataa gtaaaaatat
     1501 caaagaaagt atcaagaagt gctttttaaa tgctttcata tttaaaaacc tccaatttgc
     1561 caacttactt taacttattt ggttttctag ttgtaactac ttttattgta atggtaaata
     1621 tatcaaattg caatattctc atataatatt cttatttaac tataatgaat ttatcattat
     1681 ctatttaaat accatgtcta ataccaattt cttataaatc ataaaagtat aattaacacc
     1741 ataattctag tgattaattt tatttcaaaa aatgtaataa cactaccaat attttaaata
     1801 tcaatagtgt caaaaatttt ttcagattaa ttaaatcttt atatatattt tataataatc
     1861 caaatcatac taaacattta gacagaaaac ttttttcttt aacagtaaat gcttttgttt
     1921 gaatatcaga ataattcttt gaatatatat ctataaaacc atgatttgca tctaaaatct
     1981 caatttggat attttgtttt ctagaaagtt ggcaagaaat agaattaaca tcaaatgaat
     2041 cgtactttgc aaatactaac aaagtaggac attttggaat tacatccatg taatctctga
     2101 tacgagaacc ataacaacag attatgccat cacaaagaga atcttcacta catctccatg
     2161 caatagttgc tcccacgcta tacccagcaa taaatacata tttatactct gattttaaat
     2221 catttataag cttttcaact tttttataaa tatcaaaacc aacattattt ataaaaaaat
     2281 catatgcctc ttgaactttt gaatatggaa aaactctttt ttctcccaga agttcaggac
     2341 aaaatacatc atatccatat ttataatact tttgacaaac atcttctata aagctattaa
     2401 tcccatagat ttcatgcaaa acaacaatag ctacatcact tttatgtata tactttatca
     2461 gattatccac cacctttaca aaatagcaaa ttttttatac ttagtttaat atattattct
     2521 ttacatgtca attcactttt ttaaaaccaa atttattttt ggattaatca cttgttttta
     2581 aaaattgtgt attattactc ttaaattatt ccctattgtt aaaaacataa ttgttagaaa
     2641 taaaaaatat agtataaata ataaaaaaat atctcactca taactttaaa aattataagt
     2701 gagatactct taaaatctac tttatattaa atttattttc caaaatatct atttaataat
     2761 cctaagaatg ctttaccatg tctagcttcg tctttacaca tttcatgtac tgtatcatgt
     2821 atagcatcta atcctaactc tttagctttc ttagctaatt ttaattttcc atcagttgca
     2881 ccatattcag catcaactct aactcttaag ttttcttttg tatctgcaac tacaacttct
     2941 ccaagaagtt ctgcaaattt agcagcatgc tcagcttctt caagagctat tctcttataa
     3001 gcttctccta cttctggata accttctcta tcagcttgtc tactcattgc taaatacatt
     3061 cccacttctg tacactcacc aacaaagtta gctcttaatc cttcgattat ttcttcatct
     3121 acaccttgag ctactcctat tctgtgctca tcagcccaat ttttttcgcc tttcatttct
     3181 tcaaatttat cagctccaac tttacatact ggacattgtg ctggtgcagc atctccttca
     3241 tgtatatatc cacatactgt acaaacaaat ttcttcataa ttaatttcct cctaaatttt
     3301 atatatcaaa ttaaattttt tatttattaa gttagtctat atatacccgc accatatacc
     3361 tttaaacaat tttttttaaa gttttttcta agtacttatt ttccctgtgt caatagtatg
     3421 tttctataat aatgtccttt taattttatc aattctttat gatttccaga ttctacaatt
     3481 ctaccatttt ctaaaactat tatattatca caatttgata tagtacttaa tctatgagct
     3541 ataattatac ttgtttttcc ataagttaaa ttatttattg cctcttgaat tttagcttct
     3601 gttgctatat ctatgttgct tgtagcttca tccaaaataa gtattggagc attttttaat
     3661 accgctctag caatacttat taattgtttt tggccttcac ttaaatcttc acccctatct
     3721 gataatacag tactatatcc ttcacgcatt ttaactataa atttatgtga atttgctatt
     3781 ttactagcat atataacttc ttcatcagta gcctccagat ttccatacct tatattttcc
     3841 ataactgtat cattgaatat ataaccttct tgtgggacta caccaaaaca cgttctcaaa
     3901 tagtttctgt caatagaata aatatcctta ctatctaaaa atatacttcc ttcatctata
     3961 tgataaaaat ttgtaattag atttataatt gttgtttttc cacttcctgt ctctcctata
     4021 atggcatttg atgtaccagc tttaattttt aggtttatat tatctaaaat tttctttttt
     4081 gaatcataag aaaaacttac atttttaagt tcaatttctc cctttacaaa atgagagttt
     4141 gaattaccat ctttattatc cttaagttca cttttaaaca tattatccaa ttcttgttct
     4201 tctttttcat ctataatttc aaaaactctt tcacatccag ctatggctga aagtaatgta
     4261 ttaaatagat ttgccaattc atttaaaggt cttgtaaatt gtcttacata acttaaaaaa
     4321 ctagctataa cccctatggt tataatatct ttagatgcaa gaacacctcc tataatacat
     4381 atagttgcaa atcctatatt actaatcaca ttcataatag gcattaaaag agaagtataa
     4441 atttgagatt taagggaaat ctttagaagt ctatcattga tttctttaaa atcgtttaca
     4501 gattttattt cataattaaa agattttact acatttatgt ttgatatcat ttcttcaata
     4561 tgagcattta gttttcccaa ctcaacttgt tgttgtttaa aaaaaactct agttctcttt
     4621 gcaataaaat tactcacaaa cattactgtc ggtattaata ttacactgca aaaagttaaa
     4681 attggactta atacaaacat cataataaaa gtcatcaata tgtttaagat tcctgtaatt
     4741 aattgtacta gtgaatttga tattcctgta ctgatattat caacatcatt tgttactctg
     4801 ctcataatat ctccagtaga actcttatca aaaaaggtta ttggtagact tttaaatttt
     4861 aaaaataaat tactcctcat ctctttaact atactttggg ataatctagc tgtacatatc
     4921 cccttagaca gtttaactga aaactcaatt atataaaatg aaagtaaaat aattattcct
     4981 tttaagaaaa tggattttga ataagtatca atatctatta tgtcaactac ctttccaata
     5041 gtgtatggga ttacagttga tataattgaa tcaattatta ctagcaaaaa aataaatcta
     5101 gtaatcttct tatctttctt aaagtacata aaaattcttt taaaagtttt tttaaaatct
     5161 ttaggctttt cttttttctt taatctactg accttttgat ttccagtact taaattcaat
     5221 atacttcctc cttaatttac atcttcactt actaaattct gcaacgtaaa aattttttta
     5281 taaattggag aactaaccag taaatcttca tgtgtagcaa atccaaccac ctctccctca
     5341 tccaagacta aaatcttatc acagtcaatt atagaactaa tcttttgtga aatagttatt
     5401 actgtacatt tcttaccttc attttctctt aaattgctta tatgatcttt tatattcttt
     5461 cttataattg tctctgtatt aatatccatt gcactaaaac aatcatcaag aattaatatt
     5521 tgtggttttt ttgctatggc tctagctata gtaattcttt gtctttgccc tcctgataga
     5581 tttttacccc tttgaaataa aacttcatca taagaatttt cgagttttgt tataaattga
     5641 tttgcatttg ctatttcaca tgattttata acatcctctt tgcgtatatt tctatttccc
     5701 ataactacat tctccaatat actttttcca aacacactac tattttgaaa tactacacct
     5761 attctttctc ttaaactttc ttcagaaata ctgtatatac tatgtcctcc aatcttaata
     5821 tcgcctttat caattatata taatttttga attaaattta ctaaagaagt ctttcctgaa
     5881 ccagtttgac caatcacacc aatattttga ccttcttcta ttttaaaaga tatgtttttt
     5941 aatatttttt cacctgtatt tttgttatac gcaaaatcca cattttcaaa aattatatca
     6001 caatttgcaa actttaaatc tttttctcca atatctgaac ttttatctct ctcaattact
     6061 tcttcatcct catcaatcaa aacctcactt attctctcat aagacgcttt tgctctaaca
     6121 aaaacattaa aaacatgtga aatcattata agagatgtta atattctagt catatagttt
     6181 acataagcaa ctataatccc aattttaatt tctcctgaat aaattaaata tcttccaatc
     6241 agtaatatta ttattattga aacattaatg attattgtaa ttgtaggtct aaaaaatgac
     6301 atcatagttg tagcttttct actattttta aaaagctcat aatttaactc ttcaaaactt
     6361 ttgttctcat aatcgtatct gcaaaatagt ttaactactt ttattccact taaaaactgt
     6421 cttaattttg aatttacttt atctgtactt tcttgtacat ttttaaataa tctataccct
     6481 accttcatat taagcaatat tataataaat ataattaaaa ctgcaattag aataattatt
     6541 gacatcttta agtttaattt tatggctata attaaacttc ctatgcacat tattggtgac
     6601 tttataaaaa ctctcatcat cccattaacg aattgttgca tttgattaat atcattagta
     6661 attctcgtta ttaacgaagc tctaccaaat ttatctattt gttttaacga gtatgtattt
     6721 atcttttcaa atacatcctg tcttaaataa taagaaaaat gctgagaaac tcttgccgag
     6781 attacacttc ttattattgc aaatattgca cctaaaaaag ttatacctat cattataact
     6841 cctgttgata gaatgtaatc taagttctta tttgaaactc ctatatctat tatttttgcc
     6901 attatagttg gttgagctaa atcacaaaaa gcttccagtg tcaaaaaaaa tactgctgtt
     6961 agaaataata ctatatattt ttttatgtat tttttaaaat acctcattca atcatccctt
     7021 catagaattt tctcattacc aaaagtatat cataatggct aatttaattg attatatcaa
     7081 ttataaatat
//
LOCUS       DAFNNB010000165         7047 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0170, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000165 DAFNNB010000000
VERSION     DAFNNB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0170"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            175..1224
                     /locus_tag="L3M60_003100"
     CDS             175..1224
                     /locus_tag="L3M60_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6227732.1"
                     /translation="MFKKIVAIASSIIVSTICLTGCDFNSNDKKDESSSSKNLDKVTI
                     AEVTHSVFYAPQYAAITKGFFEDEGIKIDLINTQGADKTMAALISGEANVGLMGPEAS
                     IYVYNQNKNDYAVNFAQLTKRDGSFLVARKKMPNFSYNDLKGTEILGGRKGGVPLMTF
                     EYVLKEKGLTIGENLKAGNVNVRTDVQFGVMAGAFAGGEGDFTTAFEPTATAMEKEGS
                     GYIVTSIGKDSGEIPFTAYSTTRSYMKNNKDLIQRFTNAVYKGQLWVQQASSKEVAKA
                     MQPFFEDISLEDLTTVVDRYKSIDAWSQNPVLKEEGLKKLMTVMKEAKELDKEAPYKD
                     IVNTEFANNAIKNIK"
     gene            complement(1373..1720)
                     /locus_tag="L3M60_003101"
     CDS             complement(1373..1720)
                     /locus_tag="L3M60_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HBN6227733.1"
                     /translation="MHENKKNLLDGSLAQYNDVAVPKIPVFAGNDITSEVYYFKPNQV
                     LNAHRHPNGEQIFVFLKGEGKMKLGEHECDVKNGDTVFVPTGEWHEITNGSNEEMVAV
                     QITKINAGAEYRG"
     gene            complement(2175..6341)
                     /locus_tag="L3M60_003102"
     CDS             complement(2175..6341)
                     /locus_tag="L3M60_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HBN6227734.1"
                     /translation="MATVSGQIVFDRNRSATIDSGDSGIANVPVVLQDIATGVRLTVL
                     TNASGNYVFTNVPNGNYRIVEAFGTTGGVPTPGNFAAAIVGPVPIAEIPPISSVINPP
                     IGSTNLDGTTPNTLLITVAGSDITNQNILNGPVIYTPIQNILDSCVSVSNINLIIDAD
                     NGTFGFFPPGTPSNTGPEIEPYPNVTPDFTYVVPDPTKFTPLDGEYTVQNIMTNAMSN
                     VIGAWWRIADHTTGNETGRMMVVNGFNPGAVFFKSTVQVTPNTNFLFSTWILNLFKVT
                     GYPPPQLGVRIFDQNNNILYQATLGAQIPVNTNAPEWKEIGTVINSQNNNQLTVEFFS
                     EGESVIGNDYAIDDVSLREITVPTFTPIKTSNTETANIGDIVTYTVTLDNTCNSPLTN
                     VFFRDIIPNGLKFIPGTVTVDGISVPGVDPNIGFQLPDIGGGTRIEVTFNVVVESIPN
                     PNPTNNIATIDYSYTPVEGGIPNNFSVDSNPVPVEVISADIEVTKLSEPSIVNPGEEL
                     IYTIKVVNNGPFPSENVVLTDDVPDSIINSEYSLDGGVSFQPWNGSLNIGTLELGATR
                     VIIIRGIVNPTTVGIIRNTAVVSSTTADPNLNNNTSTIETEVNLLADILVMKTAEPNS
                     AVPGTLLRYTIQVANLGPANAENVILNDDIPDVILNPEYSLDGGVSFQPWNGSLNIGA
                     LNSGISLTVLIQGTVSLNSSEYIVNTATVSSTTPDPDLSNNISTIITPVNPQAGISII
                     KVANEDVAVPGEEFVYTIEIFNEGPSNAMNVVLTDNIPDVILNPEYSLDGGASFQPWN
                     GSLNIGTVAPGQLIRIIIRGLVSSTATGNITNTAEVSAEVPEPVTDSSTVITPVVASA
                     DIEVIKTSNMDTAVPGEIFSYTITVTNLGPSAAQSIVLTDDIPDIILNPEYSLDGGVS
                     FQPWNGNLNIGTLDTGEIRSIIIRGTINQTSVGTIINTATVSSTTPDPNPDNNTSTVE
                     TDISPLADISVIKGNEPLAIQGERFIYGIEIVNAGPSFSENIVLTDNIPASILNPEYS
                     IDNGVTFQPWTGSLNIGTLDAGEIRNVIIRGTVSQTAVGTIINTATVSSTTPDPNLNN
                     NTSTSEAEVSSSADISVVKRSNQNVVVPGDVLNYTIEVRNAGPSTAQNVTLTDNISAS
                     ILSPEYSIDNGVTFQPWTGSLSIGTLYAGEIRNIIIRGIASETAIGTIINTATVSSTT
                     PDPNPNNNTSTSEIDISSSADISIIKLANKTEACVGEQIDFVIVVSNAGPENAQNVTL
                     IDNVSDKLKKTVFSLDRGVSFQPWTGSINIGILPAGTLRVILLRGLIKSTCLDKLTNI
                     AEVTSTTPDSDLNNNISRVQVEIKQCCSNDCCCNCCCNNCCNCFFNDCCNDCGYKDCC
                     CNCCKNDFWC"
BASE COUNT         2309 a         1090 c         1017 g         2631 t
ORIGIN      
        1 aataatcaat ataatttatc caatattacg cattattaaa aagactaaac ataaaactga
       61 aacttattat taagtttttg tagaaatatt ataagtatag atacattttt gtaacaacta
      121 caatgaatat ttaatactat atactattcg tatgtcttat gggaggtatt tattatgttt
      181 aaaaaaatag tcgcaatagc aagctctatc attgtaagta caatatgtct aactggatgt
      241 gattttaact ctaatgacaa aaaagatgaa tcttcttcgt ctaaaaatct ggataaagta
      301 actatagcag aagttactca ctctgttttt tatgctccac agtatgctgc tattacgaaa
      361 ggattctttg aagatgaagg tataaagatt gaccttataa atacccaagg tgcagataaa
      421 actatggcag ctttaatttc aggtgaagca aacgtaggtt taatgggccc tgaagcatct
      481 atatatgtat ataatcaaaa caaaaacgat tatgcagtga actttgctca attgacaaaa
      541 agagatggta gttttctagt tgctaggaaa aaaatgccta atttctctta taatgattta
      601 aaaggaacag aaatattagg tggtagaaaa ggtggagttc cactaatgac ttttgaatat
      661 gttctaaaag aaaaaggtct tactattgga gaaaacctaa aggcaggcaa tgttaatgta
      721 agaactgatg tacaatttgg tgtaatggct ggagcttttg caggtggaga gggggacttt
      781 acaacagcat ttgaacctac tgcaacagca atggaaaaag aaggtagtgg atatatagtt
      841 acatctatag gaaaagattc tggagaaata ccttttacag catactctac tacaagatca
      901 tacatgaaaa acaataaaga tttaattcaa agatttacaa atgctgttta taaaggtcaa
      961 ttatgggttc aacaagcatc tagcaaagaa gtagctaagg ctatgcaacc tttctttgaa
     1021 gatatatctt tagaagattt aacaacagtt gtagatagat ataaatcaat agatgcatgg
     1081 tctcaaaacc ctgtattaaa agaagaaggt ctgaagaaat taatgactgt tatgaaggaa
     1141 gctaaagaat tagataagga agcaccttat aaagatatcg taaacactga gtttgcaaac
     1201 aatgctataa agaatattaa ataaaatata ttacttttgc ctagatattt tctcgatttt
     1261 atgattaaaa ttaacaaaaa tataaaaaga gccttttaaa agactctttt tatatttgag
     1321 attaaagttt atataataaa atagaatcaa agtactaatt gtacttatat atctatcctc
     1381 tatattctgc tccagcatta atttttgtaa tttgaacagc taccatttct tcattgcttc
     1441 cattagtaat ttcatgccat tctcctgttg gtacaaatac agtatcgccg ttttttacat
     1501 cacattcatg ctcaccaagt ttcattttac cttctccttt taggaacaca aatatttgtt
     1561 ctccattagg atgtctatga gcattaagaa cttgattagg tttaaaataa taaacttctg
     1621 aagttatatc gttaccagca aatactggta tttttggaac tgctacatca ttatattgag
     1681 ctaagcttcc atctaataga ttctttttat tttcatgcat actagaaata cctcttttct
     1741 atattatata tttactatta ttattccaat ataagaaaat agtattctca tcttgcataa
     1801 taggaataaa attcttttat ttttaaatac taaggattat attattttta tagttataga
     1861 aatataagct ttatagaata taaaaagtta acataatata ataaattcta aaaaattttt
     1921 agatatctaa actttaatat aaataaaata tataaatatt ttttataaaa attaaaatta
     1981 tttaatacgt ttaaaattta aatagaataa ataattttaa tgaattaaat aggaccactt
     2041 cattttaata actagtatta cgaatgtggt ccatatcgaa aatagaaaac ttattaatat
     2101 ttaaaaaata ttttactcat atatgaattt tttatttcag tttaatttaa gatatacaaa
     2161 attattgtat aaaatcaaca ccaaaagtca tttttacaac aattacaaca acaatccttg
     2221 taaccacaat cattacaaca atcattaaaa aaacaattac aacagttatt acaacaacaa
     2281 ttacaacagc aatcgttaga acaacattgt tttatttcta cttgcactct tgatatatta
     2341 ttgttaaggt ctgaatctgg tgttgttgaa gtaacttctg caatatttgt taatttatca
     2401 agacatgttg attttataag tcctcttaat agaataactc ttaaagttcc agctggtagt
     2461 atacctatat taatacttcc agtccaaggt tggaaactta cacctctatc aagtgaaaat
     2521 actgttttct taagcttatc tgaaacattg tctattaaag taacattttg agcattttct
     2581 ggtcctgcat ttgatactac tattacaaaa tctatttgtt ctcctacaca tgcttctgtt
     2641 ttatttgcta attttattat agaaatatct gctgatgaac ttatatctat ttctgatgtt
     2701 gaagtgttat tatttggatt tgggtcaggt gtagttgaac ttactgttgc tgtatttata
     2761 attgtaccta ttgctgtttc acttgctatt ccccttatta taatatttct tatctctcca
     2821 gcatacaaag tgcctatact aagacttcca gtccatggtt ggaaagtcac tccattatct
     2881 attgaatatt ctggacttaa aatagaagct gatatattat ctgttaaagt tacattctga
     2941 gcagtagatg gtcctgcatt tctcacctca attgtatagt ttagtacatc tcctggtaca
     3001 acaacattct gattgcttcg ttttactaca gaaatatctg ctgatgaact tacctctgct
     3061 tctgatgttg aagtattgtt atttagattt gggtcaggtg tagtcgaact tactgttgct
     3121 gtatttataa ttgttcctac tgctgtttga cttactgttc cccttattat gacatttctt
     3181 atctctccag catctaaagt acctatatta aggcttccag tccatggttg gaaagtcact
     3241 ccattgtcta ttgagtactc tggatttaaa atagaagctg gtatattgtc tgtcaagact
     3301 atattttctg aaaaagatgg tcctgcattt acaatttcaa ttccataaat aaatctttca
     3361 ccttgtatag caagtggttc gttgcctttt atcacagaaa tatctgctaa tggacttata
     3421 tctgtttcca ctgttgaagt attattatct ggatttgggt caggtgtagt tgaacttact
     3481 gttgctgtat ttataattgt gcctactgat gtttggttta ttgttcctct tattataata
     3541 cttcttattt cccctgtatc caaagtgcct atattaagat ttccattcca tggttgaaag
     3601 cttactcctc catctaatga atactctgga tttaaaataa tatctggtat atcatctgtc
     3661 aaaacgatac tctgagcagc agatggacct aaatttgtaa cagttatagt ataactaaaa
     3721 atttctcctg gaactgctgt atccatattg ctagttttta ttacttctat atctgctgat
     3781 gcaactacag gagttattac tgttgatgaa tcagtaactg gttctggtac ctctgcactt
     3841 acttctgctg tattagtaat attccctgta gcagttgaac ttactaatcc ccttatgata
     3901 attcttatta attgccctgg tgcaactgta cctatattaa gacttccatt ccatggttgg
     3961 aaacttgcgc ctccatccaa tgaatattct ggatttaaaa taacatctgg tatattatct
     4021 gttaaaacta cattcatagc attagatggt ccttcattaa aaatttcaat tgtatataca
     4081 aactcttctc ctggaacagc tacatcctca ttagccactt ttataataga aatacctgcc
     4141 tgtggattaa ctggcgtgat aatagtagat atattgttgc ttaaatctgg gtcaggtgta
     4201 gttgaggaaa ctgttgctgt atttactata tattctgaag aatttaaact aactgttcct
     4261 tgtattaaaa ctgtcaatga aattccagag tttaaagctc ctatattaag acttccattc
     4321 catggttgaa aacttactcc tccatccagt gaatattctg gatttaaaat aacatctggt
     4381 atatcatcat ttaatattac attctcagca tttgctggtc ctaagtttgc tacttgaata
     4441 gtatatctta ataaagtacc tggaacagct gaattaggtt ctgctgtttt cattaccaaa
     4501 atatctgcca ataaattcac ttctgtctct attgtagatg tattattatt aagatttggg
     4561 tctgctgtag ttgaagatac tacagctgta tttcttataa ttcctactgt tgttggattt
     4621 acaatacccc taattataat aactcttgtc gctcctaatt ctaatgtccc tatatttaaa
     4681 cttccattcc atggttgaaa acttactcct ccatccaatg aatattctga atttataata
     4741 gagtctggta catcatctgt taaaaccaca ttttcagatg ggaatggtcc gttatttact
     4801 acttttatag tgtatattaa ttcctctcct ggattcacta tagaaggttc agataatttt
     4861 gtaacttcaa tatctgctga gattacttca acaggaactg gattagaatc tactgaaaaa
     4921 ttatttggta taccaccttc tacaggagta taggagtaat ctatagtagc tatattatta
     4981 gtaggatttg ggttaggaat actctcaact acaacattaa aagttacttc aatccttgtt
     5041 cctcctccga tatctggaag ttggaaacct atattagggt ctactcctgg tacagatatt
     5101 ccatcaaccg ttacagttcc aggtataaat tttaaaccgt ttggaattat gtccctaaaa
     5161 aatacgttag ttaaagggct attacaagta ttatctagtg taactgtata tgtgactata
     5221 tcgcctatgt tagcagtttc tgtgttactt gtttttattg gagtaaaagt tggaacagta
     5281 atttctctta aggaaacgtc atctatagcg tagtcatttc caataacact ttctccctcg
     5341 ctaaaaaatt ctactgtaag ttgattatta ttttgagaat taattacagt acctatttct
     5401 ttccactctg gtgcatttgt attaactgga atttgagctc ctaatgttgc ttgatatagt
     5461 atattattat tttggtcaaa tattcttaca cctagctgtg gtggtggata tcctgttacc
     5521 ttaaaaaggt ttaaaatcca agtactaaat aaaaaattag tgttaggtgt aacttgtact
     5581 gttgatttaa aaaatacagc tccaggatta aaaccattta ccaccatcat tcttcctgtt
     5641 tcatttcctg tagtatggtc agcaatacgc caccaagctc ctataacatt actcattgca
     5701 tttgtcataa tattttgtac tgtatattct ccatctaaag gtgtaaactt agttgggtct
     5761 ggtacaacat acgtaaaatc tggtgtcaca tttgggtaag gttctatctc aggccctgta
     5821 ttacttggtg tgcctggtgg gaagaaaccg aatgttccat tatctgcgtc tataataagg
     5881 tttatattag atacagatac acaagaatcc aatatattct gtataggtgt ataaatcaca
     5941 ggaccattta atatattttg atttgttata tctgaacctg ctactgttat taatagtgtg
     6001 ttgggtgttg taccatctaa gtttgttgaa ccaataggag gatttatcac agaactaata
     6061 ggtggtattt ctgcaattgg aactggacct actatcgctg ctgcaaaatt ccctggtgtt
     6121 ggtactcctc ctgtagttcc aaaagcttct acaattctat agttaccatt tggaacgtta
     6181 gtaaaaacat aatttcctga tgcatttgtg agaactgtga gtctgactcc agttgctatg
     6241 tcttgtaata caactgggac attggctatt ccagaatctc cagaatcaat ggtagcactt
     6301 ctgtttctat caaaaactat ttgtcctgat actgttgcca tattatcact cctttgagat
     6361 tataaaaatt tgtgttcata atatattatt ctttaaaatt cttttatgtg acattttaag
     6421 cctataagtg cacttttttt ctataatagc taacatctat ttttgctgtt tgaaatatcg
     6481 atatcggttt tttaatattt tattatatat gtaaagaaag tatattgtat tataacttaa
     6541 aaattatcaa ataatagcaa atggcataaa atgtattatt ttatgccatt ttttaataat
     6601 caagtatttt caataaatac ataattataa aactgtgttt tgtcagtgaa atgtcagtga
     6661 tttttttgtt attttttact tttttatttt gaacaaattt aaaaaaggct tttatattac
     6721 aaatcaccaa acaaaatatc ataaatttta gacttaatta cttaagacat tctgagatat
     6781 aactttctgt attttatttt tgtctaattg ggtttagaga gtttattttt ctcaaaaata
     6841 acaagctttt atattggttt ttaaataggt ttaaattttt actacgatag tttgtacctt
     6901 tctaatcaaa tcgagcttaa aatctactta aatgcataat ttggtacttt tacctaaaac
     6961 taataatttt aattaacatt atttttatta tgtaaactaa aagaattttt cttaatttta
     7021 tatataattt gaataattaa tataatc
//
LOCUS       DAFNNB010000166         7029 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0171, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000166 DAFNNB010000000
VERSION     DAFNNB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0171"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            280..975
                     /gene="cas6"
                     /locus_tag="L3M60_003103"
     CDS             280..975
                     /gene="cas6"
                     /locus_tag="L3M60_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBN6227735.1"
                     /translation="MIKCLEMSVKVYLLENISLDNTSKEIRKLIDDTLIIEEKYKEFH
                     NKAGFKNYSFNLLYPLEKDKVYKKDCIYTFIIRTVDSELGMYFMNNLKNQYSDKIKVI
                     TMSKKVIPKKHIESIYSVTPIIAKFEDGYWRSNKTVEDLEKRIRENLIKKYNEFTNSK
                     IDEDFELFQVIKFINRKPISTKYKNISLLGDKISFKVSENDMAQEIANFALGVGVGEI
                     NSRGFGFVNYRWL"
     gene            966..2726
                     /locus_tag="L3M60_003104"
     CDS             966..2726
                     /locus_tag="L3M60_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cse4"
                     /protein_id="HBN6227736.1"
                     /translation="MVIGGENLLKDCLEIFEKEYKSLGNRLITDSYVLSYGTYILVKN
                     FSNIDILEVSKNNVDSTEDIYHKFCELDYLSKLVSIDKSVDSKKIIHSNNYLSFFVKK
                     ENLRPDKNNNVKLTEEIIDNYYDALLNPRIKYKSDKRKKEIYDSIENKYGKVDENKLN
                     KIRLWIKTNIFDLVESVKKDKNYLKIFFDEKIELYNDENQKYLYPNIYNKNDFNVIID
                     GKNYGLPNDNMQLNEKKPFLKLKTRIRNSTPYLISDDEVLLQKAFFDYLMNKAANGEE
                     VVYISSEKGIHRKTDIPNEFSGYILRIKKGKELEIHDFDTVVNLNTKIKRINVECIIP
                     IEYERHNLKPNLEIGYINNLIKLEKTIDTIFFNKFMCNSYFKEIKNINLSDLVLRDIL
                     IRHREGLYTWFYKGRDNIVKQTFSNYSMGIIENSIKNNNYIRAKEQYNLRIAIINYFH
                     GGSNMADKIKVISESLFEKINSIKTKCIQSDEEYYFAVGQLASYFISKNKSKNKNHSL
                     INPILNAQNRKKIMLEIEKLFKKYNYDISYDNNRRFKNLYSMIVGYEVEEKKVNRDLL
                     IGGYLYYSLIYTKSEENENE"
     gene            2713..3708
                     /locus_tag="L3M60_003105"
     CDS             2713..3708
                     /locus_tag="L3M60_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HBN6227737.1"
                     /translation="MKMNRRVYGVLGIKSIMSNWNADFSGYPKTTSTGEVFGSDKALK
                     FPMKKMWENEGEKVLYVKSLKIDESKKGEIKIRPKSLKERYEELFDIEELKLEKNQQK
                     VLENLFKAIDVKNFGATFAEEGNNIGIMGAVQIGQGFNKYEDINVEEQQILSPFRDPR
                     AKDKKKSTNEEEKEDANSTTLGKKIVSDEAHYFYPFSINPKAYEVFVNMGVTDGYTKN
                     DYKKFKEAALVSATAFNTNSKLGCENEFALFVETDEELYLPDLAQYITFEKREKSKDL
                     ICINIIHLIAELKDDIKSVEIYYNPYKIEIKPFKDEILPKIDKVKFMNIFTRKEV"
     gene            3710..4450
                     /gene="cas5b"
                     /locus_tag="L3M60_003106"
     CDS             3710..4450
                     /gene="cas5b"
                     /locus_tag="L3M60_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated protein Cas5"
                     /protein_id="HBN6227738.1"
                     /translation="MEVLKFNLSGKTAFFKKPDVNSIIYFTYGNIHKIALMGILGACL
                     GLGGYNQQSKEDEYPEFYSLLKDIKMSIVPKHEFGYINKKVHIFNNSVGYASKEEGGN
                     LIIKEQWLENVSWDIYIQLEDSTTSRELKRRFLSNKFVYIPYLGKNDHIANISEIEII
                     TDFYKTEDINRVDSFFIREDFEFEKSYFDDNDDYEEPFKYQEKLPYRLHRMTNQYILE
                     TIIFTNMSVKLINKKEVYQVKGLNLIFI"
     gene            4474..>7029
                     /locus_tag="L3M60_003107"
     CDS             4474..>7029
                     /locus_tag="L3M60_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBN6227739.1"
                     /translation="MYFKKVELFDISNNINNCKNIYAHINKDRKPETLKEHSDLSYSY
                     LMKLCVKKDLSSCLERIENKFFKKDSFLSKNFYREIILNMVYMHDIGKINSSFQFVKM
                     QNKNFKKISLRYTKHSFISSMLYFDYYFEKLKKYDFEEKKILSLFLMINTYTISKHHG
                     VLKSFKEFLFLFKDEFINIKENFKDLFKNNYNLELKSVPKTIEKMVRGVEGYLDDSHI
                     KDNLAIDLYIYSKLMFSIVTSCDFYATSEYNSGNSIDGFGTIDNYNKYYDVLKKSEIY
                     KGMKKYREYLNNNSECPYKENDINRLRTEMSIEAEDAITHNTRKRIFYLEAPTGSGKT
                     VTSINLALKSIELNQSLKKIFYIFPFNTLVEQTKDVFINEIFNSVKDIQKDVVVINSI
                     TPIQTEDKEEDNKNVEYCVTKQEIDYDKSLLNRQFLHYPIVLTTNIGFFNYLFGVSRE
                     ECFPLIHMINSVIILDEIQNYKNEIWKEIILFLEKYADILNIKFIIMSATLPRLNKLG
                     CNDDNFAYLVKNREKFFKNHLFKDRVNISFEMLNSEINDIEEISEKVIEEAEIYNKIL
                     IEFIKKSSALEFYKNIKEKLKYKLEDVFLLTGDDNKLERKKIINKIKDMNSVILVATQ
                     VVEAGVDIDMDLGFKDISTIDSDEQFLGRINRSCKKLNSKVYFFNLDDASKIYKKDVR
                     KERHLTLNEESNRKIILDKDFEKYYDKIIARIEENKHKHNDKNIEVFIKDIVGNLNFT
                     EVKKWMALIPDLKEYTIFLNTIVKDESGNMIVGSDVWYEYISLLKNDNVEYSEKRVKM
                     SEIMEKLDYFTYKVQKFDNSFNDLVGDIFYIDDGSKYFTEGKFDRSKFNQNEFL"
BASE COUNT         3094 a          547 c         1087 g         2301 t
ORIGIN      
        1 tatttaatta tttacttgtt ttaaatttta ttaacttaaa aaatgtaaga aatttattat
       61 caataatcta aatatagaaa ttaaaaatga taaagagatt gtaattatat ttttttgaaa
      121 ctttgtgatt ttatttgtcg aataaaaaat aaaaaaattt aaaggaaaaa gttaaaaaat
      181 aaagaatttt ataactataa attatatttt agagtttatt gttattaaat agatgaaaaa
      241 atcctaatgt ggattatcag aaaatgagga ggttgttaat tgataaaatg tcttgaaatg
      301 tctgtaaaag tgtatcttct tgaaaatatt tctttagata atacatcaaa agaaatacgt
      361 aaattaatag atgatacatt aattatagaa gaaaaatata aggaatttca taataaagct
      421 ggatttaaga attattcttt taatttatta tatccattag aaaaagataa ggtttataag
      481 aaggattgta tatatacttt tattattaga actgtagata gtgaattagg aatgtatttt
      541 atgaataatt taaaaaatca gtattctgat aaaataaagg ttattacaat gagtaaaaaa
      601 gtgatcccta aaaaacatat agaaagtatt tactctgtca ctccaataat agctaaattt
      661 gaggatgggt attggagaag taataaaaca gtagaagatt tagaaaaaag aatcagagaa
      721 aatttaataa aaaaatataa tgaatttact aacagtaaaa tagatgaaga ttttgaatta
      781 tttcaagtta ttaaatttat aaataggaaa cctatttcta ctaaatataa gaatatatct
      841 ttacttggtg ataaaataag ttttaaagta tcagaaaatg atatggctca ggaaattgca
      901 aattttgcac ttggagttgg agtcggagaa attaattctc gtggatttgg ttttgtaaac
      961 tatagatggt tataggaggt gaaaacttgt taaaggattg tttagagatt tttgaaaaag
     1021 aatacaaatc tttaggaaat agattgataa ctgatagcta tgtcctatcc tatggaacat
     1081 atatattggt gaaaaatttt tcgaatatag atatattaga agtttctaaa aataatgtgg
     1141 atagtacaga agatatatac cataaatttt gtgaactgga ttatttatca aaactggtgt
     1201 ctatagataa atcagttgat tccaaaaaaa taatacatag taataattat ctttcatttt
     1261 ttgtaaaaaa ggaaaactta agacctgata aaaataataa tgtaaaactt acagaagaaa
     1321 ttattgataa ttattatgat gctttgttaa acccaagaat taaatataag tctgataaaa
     1381 gaaaaaaaga aatctatgat tcaatagaaa ataaatatgg taaagtggat gaaaataaat
     1441 taaataaaat aagactatgg ataaaaacaa atatatttga tttagttgaa tctgttaaaa
     1501 aagataaaaa ttatttaaaa atattttttg atgaaaaaat agaattatac aatgatgaaa
     1561 accaaaaata tttatatcca aatatatata ataaaaatga tttcaatgta attattgatg
     1621 gtaaaaacta tgggttacca aacgataata tgcaattaaa tgaaaaaaag ccatttttga
     1681 aattaaaaac aaggattaga aattcaactc cttatttgat tagtgatgat gaagttttgt
     1741 tacagaaagc tttttttgat tatctaatga ataaagcagc gaatggagaa gaagtagtat
     1801 atataagtag cgaaaaggga attcatagaa aaactgatat accaaatgaa tttagtggtt
     1861 atatactgag aataaaaaag ggtaaagaat tagaaataca tgattttgat actgtggtaa
     1921 atttaaacac taaaataaaa agaataaatg tagaatgtat aataccaata gaatatgaaa
     1981 gacataattt aaaaccaaat ttagagatag gatatattaa taatcttata aaattggaaa
     2041 aaacgattga tactatattc tttaataaat ttatgtgcaa tagttatttt aaagaaatta
     2101 aaaatataaa cttaagtgat ttagttctaa gggatatact tattagacat agagaaggtt
     2161 tatatacctg gttttataaa gggagagata atatagtaaa gcaaactttt tcaaactatt
     2221 ctatgggtat aattgaaaat tctataaaaa acaataacta cataagagca aaggagcaat
     2281 ataatttaag aatagctata ataaattatt ttcatggagg aagtaatatg gcagataaaa
     2341 taaaagttat tagtgaatct ttatttgaaa aaataaattc tataaaaacg aaatgtatac
     2401 aaagtgatga agaatattat tttgcagttg ggcaattagc atcatatttt atatctaaaa
     2461 ataagtcaaa aaataaaaac cattctctga ttaatcctat actaaatgca caaaatagaa
     2521 aaaaaatcat gttagagata gaaaaattat ttaaaaaata taattatgat ataagttatg
     2581 acaacaatag gcgatttaag aatctatatt ctatgattgt aggatatgag gtagaagaaa
     2641 aaaaagttaa tagagattta ttaattggtg gatatctata ttacagttta atatacacaa
     2701 aaagtgagga gaatgaaaat gaatagaaga gtatatggag tattaggtat aaaatctata
     2761 atgtcaaact ggaatgctga tttttctgga tatccaaaga cgacatcaac aggagaagtt
     2821 tttgggagtg ataaagcact taagtttccc atgaaaaaaa tgtgggaaaa tgaaggtgaa
     2881 aaagttttat atgttaaatc attaaaaata gatgaatcta agaaaggaga aataaaaatt
     2941 agaccaaaat cattaaaaga gagatatgaa gaattgtttg atatagagga actaaaacta
     3001 gaaaagaatc aacaaaaagt actggaaaat ctatttaagg ctattgatgt taaaaatttt
     3061 ggtgcaacat ttgcagaaga aggaaataat ataggaatta tgggcgcagt acaaattgga
     3121 caaggattta ataagtatga agatattaat gttgaagaac aacaaatatt atccccattt
     3181 agggatccaa gagcaaaaga taagaaaaag tcaacaaatg aagaagaaaa agaagatgca
     3241 aattcaacta cattaggaaa aaaaatagta agtgatgagg ctcattactt ttatccattt
     3301 tcaataaatc caaaagctta tgaagttttt gttaatatgg gtgtgactga tggatatact
     3361 aaaaatgatt ataaaaaatt taaagaagct gctttagtaa gtgcaactgc attcaataca
     3421 aattcaaagc ttggttgcga aaatgaattt gcattatttg ttgaaactga tgaagagtta
     3481 tacctaccag atctggcaca atatataact tttgaaaaga gagaaaaatc aaaagattta
     3541 atttgtataa atataattca tcttatagca gaattaaaag atgatataaa gagcgttgag
     3601 atatattata acccatataa aatagaaata aagcctttta aagatgaaat attacctaag
     3661 atagataaag ttaaatttat gaacatattt acaagaaaag aggtataata tggaggtttt
     3721 gaaatttaac ttaagtggaa aaactgcttt ttttaaaaaa ccagatgtaa atagcataat
     3781 ttattttacg tatggaaaca tacataaaat tgcacttatg ggtattttag gagcttgttt
     3841 aggacttggt ggatataatc aacagagtaa ggaggatgag tatccagaat tctactcttt
     3901 acttaaggat ataaaaatgt caatagttcc aaagcatgaa tttggatata taaataaaaa
     3961 agtccatata tttaataatt ctgtaggata tgcatcaaaa gaagaaggtg gaaatttaat
     4021 aataaaagaa cagtggcttg aaaatgttag ttgggatata tatatacaat tagaagattc
     4081 aactacaagt agagagctta aaagaagatt tttatctaat aaatttgtat atatacctta
     4141 cttaggcaaa aatgatcata tagctaatat aagtgaaata gagataatta ctgattttta
     4201 caaaacagaa gatattaaca gagtagacag tttctttata agagaggatt ttgagtttga
     4261 aaaatcttat tttgatgata atgatgatta tgaagagcca tttaaatatc aggaaaaact
     4321 tccttataga ctgcaccgaa tgactaatca atatattttg gaaactatca tatttactaa
     4381 tatgtcagtt aaattgatta ataaaaaaga ggtatatcaa gtaaagggtt taaatttaat
     4441 ctttatttaa atttataggt ttaggagaat tatatgtact ttaaaaaagt tgagttgttt
     4501 gatataagta ataatataaa taattgcaaa aatatttatg ctcatataaa taaagacagg
     4561 aagccagaaa ctttaaaaga acattcagac ttatcatact catatcttat gaaattatgt
     4621 gtaaaaaaag atttaagctc ttgcctagag agaatagaga ataaattttt taaaaaagat
     4681 tcttttttaa gtaagaattt ttatagagag attatcttga atatggttta tatgcatgat
     4741 ataggtaaga ttaattcttc atttcagttt gtaaaaatgc aaaataaaaa ttttaaaaaa
     4801 atttcactta gatatactaa acattctttt atctcttcaa tgctatattt tgattactat
     4861 tttgaaaagc ttaaaaaata tgattttgaa gaaaaaaaaa tattgagttt atttttgatg
     4921 ataaatacat atacaatatc aaaacatcat ggggttctga agtcatttaa agaattttta
     4981 tttttattta aagatgagtt tataaacatt aaagaaaatt ttaaagattt atttaaaaac
     5041 aattataatt tagagcttaa atcagttccc aaaactatag aaaaaatggt aagaggtgta
     5101 gaagggtatt tagatgatag ccatataaag gataacttag ctatagactt atatatatat
     5161 tcaaaattga tgttttcaat tgttacatct tgtgattttt atgctacatc agaatataat
     5221 agtggtaatt ctatagatgg ttttgggaca attgataatt ataataagta ttatgatgta
     5281 ttaaaaaaat ctgaaatata taaggggatg aaaaaatata gggaatactt aaataacaat
     5341 agtgaatgcc catataaaga gaatgatata aacagattga gaacagaaat gagtatagaa
     5401 gctgaagatg caattacaca taatacgaga aaaagaatat tttatttaga agcaccaact
     5461 ggaagtggaa aaactgttac atctataaat ctagcactga aatctataga gttaaatcaa
     5521 tctttaaaaa agatatttta tatatttcca tttaacacat tagtagagca aacaaaagat
     5581 gtatttataa atgaaatatt taattctgtt aaagacatac aaaaagatgt tgtagttata
     5641 aattcaataa caccaataca aacagaagat aaagaagaag ataataaaaa tgttgaatat
     5701 tgtgttacta aacaagaaat tgactatgat aaatcattgt taaatagaca atttttacac
     5761 tatcctatag tacttacaac taatataggt ttttttaact atttgtttgg tgtatcaaga
     5821 gaagaatgtt ttccattgat acatatgata aatagtgtaa ttatattaga tgaaatacaa
     5881 aattataaaa atgagatatg gaaagaaatt attttgtttt tagaaaaata tgcagatata
     5941 ttaaatataa aatttattat aatgtctgca actttaccta gattaaataa attaggatgt
     6001 aatgatgata attttgcata tctagtaaaa aacagagaaa aattttttaa aaatcacctt
     6061 tttaaagata gagtaaacat aagttttgag atgttaaatt cagaaattaa tgatattgaa
     6121 gaaatatcag aaaaggttat agaagaagca gaaatatata ataagatact gatagagttt
     6181 ataaagaaat catcagcact agaattttat aaaaatatta aagaaaaatt gaagtataag
     6241 ttagaagatg tttttttatt aacaggtgat gataataaat tagaaagaaa aaaaataata
     6301 aataaaatta aagatatgaa ctctgtaatt ttggttgcta cacaagttgt agaggctgga
     6361 gtagatatag atatggattt gggatttaaa gatatatcta caattgattc tgatgaacaa
     6421 tttttgggaa gaataaatag atcttgtaaa aaattaaatt caaaagtata cttttttaat
     6481 ttagatgatg caagtaagat atataagaaa gatgttagaa aagagagaca tttaactctt
     6541 aatgaggaaa gtaatagaaa aattatttta gataaagact ttgaaaagta ttatgataaa
     6601 ataatagcca gaatagaaga aaataagcat aaacataatg acaagaatat agaggttttt
     6661 ataaaagata ttgtgggaaa tttgaatttt acagaggtaa aaaaatggat ggctttaata
     6721 ccagatttaa aagagtatac aatcttttta aatactatag ttaaagatga atctggaaat
     6781 atgattgtag gaagtgatgt atggtatgaa tatatatcac tactaaaaaa tgataatgtt
     6841 gagtattctg aaaagagggt gaaaatgtct gaaattatgg aaaaactaga ttattttacg
     6901 tataaagttc agaaatttga taattcattt aatgatttag ttggagatat tttttatatt
     6961 gatgatggaa gtaagtattt tacagaggga aagtttgata gaagtaaatt taatcaaaat
     7021 gaatttcta
//
LOCUS       DAFNNB010000167         7024 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0172, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000167 DAFNNB010000000
VERSION     DAFNNB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0172"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            305..1240
                     /locus_tag="L3M60_003108"
     CDS             305..1240
                     /locus_tag="L3M60_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HBN6227740.1"
                     /translation="MIRYYKTIDSKLEKLSFFEDGCWINLVEPNHSEINEISNLLNID
                     VESIESALDEEERSRIDVEDNHTLILIDIPVDESDSNSSHYTTIPLGIILTEEAIVTV
                     CDAQTKILNDFIVGHIKDFFTFKKTRFLLQILHKNAAYYLHYLRKINKMTIIIEREIY
                     KSMKNKELVQLLELEKSLVYFSTSLKSNELVLNKMVRTAGIKKYPDDEDLLEDVIVEN
                     RQALDMAKIYGDILSRIMDAFSAIISNNQNNVMQILTVVTLIFSIPTIISGFFGMNVI
                     NMPFSDDPNGFWIILLISAIICIVITFFMSRNKLL"
     gene            complement(1332..3203)
                     /locus_tag="L3M60_003109"
     CDS             complement(1332..3203)
                     /locus_tag="L3M60_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBN6227741.1"
                     /translation="MKNTKFSIKKIKNISNDPKVVHTKGALLEKMGKIESAIELYKSA
                     ALDNYVKSQYTLGNIYESKKQFKEAEKWYSMAYKSGSEDAAFDLGNMYYKLDGYEYAI
                     YWYEKIANLGYLQAQNNLGVCHFKMKDFIRCEKWLKSAADNNLGKACFNLGVLYTFLE
                     KDDEAYEYYKKGSVLLDDDAKYNLAILNGKKKDNKSTVSLYKQLYKSGHMKGCFNLGM
                     IMEINDNLEDAEKYYKKSADRDDVKSEYRLAYVYDRKEELGDAIYYYEKAIEKNHTLS
                     KYRLANLYNRENEIEKAKTYYKMAAEDDITEAKNNLAGIYFEEKDYENAIKYYEDAIA
                     VGCKSSLENLGDLYYQNQDIEKAISYYSRIPNNVSCQIKLGNIYEDLNNVEEAISWYK
                     KASENGDTRSSYRLGCIYESLGNTKNARKYFEMASSKNHMNARIHLGRIYFREGKLEE
                     AKMMFDTPANENNVYAQHMVGLIYDMFYKDYVNSKFWYEKARAQGCVESIYNLGQIYL
                     KLNDDAEAEKYYKEGIKYGSKKCEYMLAGLYYKKSLDMYISLANEEYDNCEEIVEDFP
                     NLNIDFDEVLIPPFKLQEEVIDEEEYVPIYILNIKESLDSMLEGLETEMIIDEEHDN"
     gene            3396..4322
                     /locus_tag="L3M60_003110"
     CDS             3396..4322
                     /locus_tag="L3M60_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HBN6227742.1"
                     /translation="MNFSNIFIQVAVLFIIILVGYFVRKFNLLDDHCTSKLSTLTMTV
                     FLPSMIISSMQINFDSKMIQKILLLLFTSLIMYIVSIIIAFLLKYILKCNDKKDLGIY
                     QYIVVFSNVAFMGYPVIEAVLGHEAIFYTAIFNLPFNLFSFTLGIYLLSKGSTNKSFS
                     IKSLISPATIAIVIGLFLFVTGLRLPQFINEPLEMLGSITTPISMIIIGSLLANSSAL
                     DCFVNKKLYIVTFIRLLLLPVIVYFILKGWINDKMILAIPVVISSMPAAANTAIMANQ
                     YDSNITLASQCVFFTTLFSVISIPFISILLLS"
     gene            4461..4607
                     /locus_tag="L3M60_003111"
     CDS             4461..4607
                     /locus_tag="L3M60_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227743.1"
                     /translation="MDMDLLFKIVFAIVAIGVSVKIIKTVASLAFKIALILLIILFVY
                     RMFL"
     gene            complement(4712..5236)
                     /locus_tag="L3M60_003112"
     CDS             complement(4712..5236)
                     /locus_tag="L3M60_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227744.1"
                     /translation="MSKPIDITIRPIRREDAQAFNEIRRTYSVMRNTLALMSDRVDKT
                     ESMLASLGENDYMFVAETNNNGEKDVIGFAGLHVNSSPRLRHSAEIGISVAENYQGRG
                     VGKKLMEQLLDIADNWIMLARVGLEVIVDNEKGLNLYKKLGFEIEGTKKYAVIRDGKF
                     EDVYIMGRYNKNLI"
     gene            complement(5289..5513)
                     /locus_tag="L3M60_003113"
     CDS             complement(5289..5513)
                     /locus_tag="L3M60_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227745.1"
                     /translation="MIIKLTKDDFNFRFKQYEGMFFHAEIIESMESIRYKITKDKEGT
                     ELKEFGSTHIKDNMIYVPQIEAYIDLKSIK"
     gene            5710..6834
                     /locus_tag="L3M60_003114"
     CDS             5710..6834
                     /locus_tag="L3M60_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3810 domain-containing protein"
                     /protein_id="HBN6227746.1"
                     /translation="MSKKTKYFSLILFPIALLLNFIASKVPNIVEKYYSQFIDKIIVQ
                     VLSKVSGIFPFSLYEITMYIIVISIFLFLCCTLSTIFNKKKKLNIFLKNSILNILSIV
                     SIVYFLFVVLWGINYNRIPLETTLINNYNLENNTSIQSKQHSVKELTKLYKFLIIKAN
                     ETRKLTLQDKNGIVKSNTDYNGVINRAQLGYDNILDILPSVSGSYSKPKYIISSNLMC
                     YTGITGIYFPFTGEANVNIAIPDLYIPCTVEHEMAHQRGFASEDEANFIAYLTSIKHP
                     NIDFNYSGYILALNYTASALSKVDYNAYVDISAGISDSVRRDLKNESKFWQKYEGKID
                     EISNEFNNSYLKANGVTEGTQSYGKMVDLLLTYYELYPYN"
BASE COUNT         2396 a         1064 c          763 g         2801 t
ORIGIN      
        1 tatgtaaatt atgataaaaa aattatttaa tcaaatcaaa acttgattta tatacctgga
       61 aaaggtatta tattaaagag tgcttaaaat acgcatttca taaataacta tataaaaaat
      121 aataaataag gagagtgatg ggcaatggat actagtccgt tgcggagaga attttttaca
      181 ttctcatcac ttaataatat ctaaaatcaa aactaataca tatattcagt tatataaatt
      241 agttaattaa tagatactat taaatgatga gatttatata aaactgaata aaggatgtgt
      301 tgccatgata agatattaca aaactataga ttcaaaatta gagaaactta gtttttttga
      361 agatggctgt tggataaatc ttgttgaacc taatcatagt gaaatcaatg aaatttctaa
      421 tttattaaat atagatgtag aaagcataga atctgcacta gatgaagaag aacgctcaag
      481 aatagatgtt gaagacaatc atactcttat acttatagat ataccagtag acgaaagtga
      541 ctctaattct tctcattata ctacaatacc cttaggtatt atactgactg aagaagctat
      601 tgtaacagtt tgtgatgcac aaactaagat tttaaacgac tttatagttg gtcatattaa
      661 agattttttt acatttaaaa agactcgctt tttacttcaa attcttcata aaaacgcagc
      721 ttattacttg cattacttaa gaaaaataaa taaaatgaca atcataatag agcgagaaat
      781 ttacaaatca atgaaaaaca aagaattggt tcaattatta gaattagaaa aatctttggt
      841 atacttctct acatcactaa aatctaatga acttgtttta aacaaaatgg ttagaactgc
      901 tggtataaag aaatatccag atgatgaaga cctattggaa gatgttatag ttgaaaatag
      961 acaggctctt gatatggcta aaatatacgg agatatattg agtagaatta tggatgcttt
     1021 cagtgcaatt ataagtaata atcagaataa tgttatgcaa attttaacgg tagttacact
     1081 aattttctca atacctacaa taatatcagg attctttggt atgaatgtca tcaacatgcc
     1141 gttttctgat gacccaaatg gattttggat aatacttctt atttccgcta taatatgtat
     1201 agtaattaca tttttcatgt caagaaacaa gctattataa aatattaata aaaaagccat
     1261 tagaactaat tgattttcat ttatcctaat ggctttttta tatttaaata tatataacct
     1321 tataatactt tttaattatc atgttcttca tctataatca tttcagtttc aagcccttct
     1381 agcattgaat ctaaactttc ttttatattt agtatataaa ttggtacata ctcttcttca
     1441 tctattacct cttcttgcaa cttaaatgga ggaattaata cttcatcaaa gtctatattt
     1501 aaattaggaa agtcctctac tatctcttca caattatcgt attcttcatt tgctaaagaa
     1561 atgtacatat ctaggctctt tttataataa agacctgcaa gcatgtactc acacttctta
     1621 cttccatatt taataccttc tttataatat ttctcagctt ctgcgtcatc atttaatttt
     1681 aaataaattt gacctaaatt atatattgat tcaacacaac cttgtgccct agccttttca
     1741 taccaaaatt tagaatttac atagtcttta taaaacatat cataaattaa tcccaccata
     1801 tgttgagcat acacattatt ttcattggca ggagtatcaa acatcatttt agcctcttcc
     1861 aatttacctt ctctaaaata aattctacct agatgtatac gagcattcat atggtttttg
     1921 ctacttgcca tttcaaaata ctttctagca ttttttgtat ttcctaaact ctcataaata
     1981 catcccaatc tataactaga cctcgtatcc ccattttcag atgctttctt ataccaactt
     2041 atggcttctt caacattatt taaatcttca tagatatttc ctaatttgat ttgacaagat
     2101 acattatttg gaatccttga ataatacgaa atcgcttttt ctatatcttg attctgataa
     2161 tataaatccc ctagattctc aagggaactt ttgcatccaa cagctatagc atcctcataa
     2221 tatttaatag cattttcata atctttttct tcaaaatata tacctgccag attatttttt
     2281 gcttctgtta tatcatcttc agccgccatt ttataataag tttttgcttt ttctatctca
     2341 ttttctctat tgtataaatt agcaagtcta tacttagaaa gtgtatgatt tttttctatt
     2401 gctttctcat aataataaat tgcatctccc agttcttctt ttctatcata tacatatgca
     2461 agtctatact cagacttcac atcatctcta tcagcactct ttttataata tttttctgca
     2521 tcttctaaat tatcatttat ctccataatc atacccaagt taaagcaacc tttcatatgc
     2581 cctgacttgt acaactgttt atataaactc actgttgatt tattatcttt cttttttcca
     2641 tttaatattg ctaaattata tttcgcatca tcatctaaaa gcacagaacc ttttttataa
     2701 tattcatatg cttcatcatc tttctccaaa aaagtatata atacccctaa attaaaacaa
     2761 gctttaccta aattattgtc tgctgctgat tttaaccatt tttcacatct tataaaatct
     2821 ttcattttaa aatggcatac acctaaatta ttttgtgctt gcaaataacc taaatttgct
     2881 atcttttcat accaatatat agcatattca taaccatcta acttatagta catattacct
     2941 aaatcaaaag cagcatcttc acttccactt ttatatgcca ttgaatacca cttttctgct
     3001 tctttaaatt gttttttact ttcatatata tttcctaaag tatattgaga ttttacataa
     3061 ttatccaatg cagcactctt atacaactct attgcgcttt ctatcttacc catcttttca
     3121 aggagggcac ccttagtatg tacgactttc gggtcattag atatattttt tatttttttt
     3181 atagaaaatt ttgtattttt cattattcgc tacctaccca ttttataaaa tatattctct
     3241 caaacattat tatacattat aatctataat taatgctata atattgaatt tttacctttt
     3301 gtcactatat tgtaacatta aattttaaat tttataatca ttagatttta ttatgtaatt
     3361 ggtatataat gtatatataa ggggagggac attttatgaa tttcagcaat atttttattc
     3421 aagttgcagt tttatttata attattttag ttgggtattt tgtcagaaaa ttcaacttat
     3481 tagatgacca ctgcacatct aagctttcaa ctttgacaat gacagttttt ttaccctcaa
     3541 tgataattag ctccatgcaa attaactttg atagtaaaat gatacaaaaa atactattgt
     3601 tattatttac atctttgatt atgtatatag tttcaattat aattgcattt ttgttaaaat
     3661 acattttaaa atgtaatgat aaaaaagatt tgggaatata tcagtatata gttgtttttt
     3721 caaatgttgc ttttatgggt tatcctgtaa ttgaagcagt tctaggtcat gaagcaatat
     3781 tctatactgc aatattcaat ttgcctttta acttattttc tttcacttta ggaatttatc
     3841 ttttatctaa aggcagtaca aataaaagtt tttcaataaa atcacttata agccccgcta
     3901 caatagctat tgttattgga ctttttttat ttgtaacagg acttaggtta ccccaattta
     3961 taaacgagcc acttgagatg ttgggaagta taactacacc aatatccatg attattattg
     4021 gaagtttact tgctaattct tcagctctcg attgttttgt caacaaaaaa ttatatatag
     4081 tcacttttat aagattattg ctgcttcctg tgattgtata ttttattcta aaaggatgga
     4141 taaatgataa gatgattttg gctataccag tagtaatttc ttcaatgcct gctgctgcaa
     4201 atactgctat aatggctaat caatatgact ccaatataac actagcttca caatgtgtgt
     4261 tttttacaac actattttca gttatatcta taccttttat aagtatttta ctcttatctt
     4321 gatttatttt tttatgtatt atataataac atattcaata aattacaaat ataaacattt
     4381 tattgtagta tattgatgga attttttcct agatatttgt caataatatc tatatgtatt
     4441 atagaatagg aggcttaaat atggatatgg atttactctt taaaatagta tttgctatag
     4501 ttgctatagg tgtttctgta aaaatcatca aaactgttgc tagtttagct tttaaaatag
     4561 cgttgatatt gctaataatt ttatttgtat atagaatgtt tttgtaaaca tttaaaaata
     4621 agttttttaa taaaatagag aagtaaaata gattatcttt aatgtaaatt ttttattaaa
     4681 gacactctat tttacttctc tattagtttt tttaaattaa atttttatta tatcttccca
     4741 taatataaac atcttcaaat tttccatctc tgattacagc atatttttta gttccctcta
     4801 tttcaaaacc tagtttttta tataaattta atcccttttc attatccaca attacttcta
     4861 atcctactct agctaacata atccaattat ctgctatgtc taaaagttgt tccataagct
     4921 ttttacctac tcctcttccc tgataattct ctgcaacact tattcctatt tctgcactat
     4981 gtctcaatcg tggagaagaa tttacatgta acccagcaaa gcctattaca tctttttctc
     5041 cattattatt agtttcagct acaaacatat aatcattttc tcccaaagaa gctaacatag
     5101 attcagtttt atcgactctg tcactcatta atgccaaagt atttctcata acactgtatg
     5161 ttcttctaat ttcattaaaa gcttgtgcat cttctcttct tataggtctt attgttatat
     5221 ctataggctt actcatattg gattccccct caaacataat ctttattttt aattaaaatt
     5281 ttacatcttt attttatact tttcaaatct atgtatgctt ctatctgagg tacatatatc
     5341 atattatctt ttatatgtgt actcccaaat tcttttaatt ctgtaccttc tttatcctta
     5401 gttattttat atctgatgga ttccatactt tcaattattt cagcatgaaa aaacatacct
     5461 tcatattgtt taaatctaaa attaaaatcg tctttagtaa gttttattat catattttta
     5521 tctactcctt atataattaa tatttttctt attattagtt gtatacatat tgcttattca
     5581 tattaatata ttataactta tcatttattt agtataaagt tataaataga tatatttata
     5641 cttattttac aatctttctt caattgatat ataatatgtt tatataaact aatgtatgtg
     5701 aggtgaaata tgagcaaaaa aacaaaatat ttttctttaa ttctctttcc tattgctttg
     5761 cttttaaatt ttatagccag caaagttcca aatattgttg aaaaatatta ctcccaattt
     5821 atagataaaa taattgtaca agtattgagt aaagtttcag gaatttttcc attttctctc
     5881 tatgaaatta caatgtacat aatagttata tctatcttct tatttttatg ttgtacttta
     5941 tctacaattt ttaataagaa aaaaaaatta aacatatttt taaaaaattc tattttaaat
     6001 atattatcca ttgtatcaat agtctacttt ctttttgtag tgctttgggg aataaactat
     6061 aataggattc cactagaaac cacacttata aataactata atttagaaaa caatacctct
     6121 atccaatcaa aacaacatag tgttaaagag cttactaaat tatataaatt tcttataatt
     6181 aaagcaaatg aaactagaaa actcacatta caagataaaa atggtattgt aaaatctaat
     6241 acagattata atggtgttat aaatagagct caactaggat acgataatat cttggatata
     6301 ttacctagtg ttagtggtag ttattctaag cctaagtata ttatttcatc aaatctaatg
     6361 tgctatacag ggataactgg catatacttt ccttttacag gtgaggcaaa tgtaaatata
     6421 gcaatacctg acttgtatat tccttgtaca gttgaacatg aaatggcaca tcaaagggga
     6481 tttgctagtg aagatgaggc taattttata gcgtatctta cttctataaa acaccccaat
     6541 attgatttta actattctgg atatatttta gctttaaatt atactgcttc tgctttaagt
     6601 aaggttgatt ataatgcata tgttgatatt tctgctggta tttcagattc tgtccgtaga
     6661 gatttaaaaa atgaaagtaa attttggcaa aaatatgaag gtaaaattga tgagatttct
     6721 aatgagttta acaattctta cttaaaagct aatggcgtta ctgaaggcac tcagagttat
     6781 ggtaaaatgg ttgatttatt gctaacttat tatgaattat atccatataa ctaaacacta
     6841 caattcaaaa ctttattcat atacagctgg tattaaatca gctgttttct atttaaataa
     6901 attatttagc tccaagcaat cttatataat ttgaaaatac ttatactatc tgtataacat
     6961 ttcaattcta actattttag ttaaaacatt tctattatct tttttaataa atattttaac
     7021 taaa
//
LOCUS       DAFNNB010000168         6680 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0173, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000168 DAFNNB010000000
VERSION     DAFNNB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0173"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     regulatory      160..281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            413..1525
                     /gene="ribD"
                     /locus_tag="L3M60_003115"
     CDS             413..1525
                     /gene="ribD"
                     /locus_tag="L3M60_003115"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBN6227747.1"
                     /translation="MNFVNQKEKDIYYMKKAIELAKNGEGFVNPNPLVGCVIVKDDYI
                     IGKGYHEKFGSNHAEVNAINSAKQSLKDSTLYVNLEPCSHYGKTPPCVDKIIQNKIKR
                     VVISTLDPNPLVCGNGVKKLRDNNIDVTVGTLEEEARDLNEVFFYYIKNKRPLCIVKS
                     AVSLDGKIATKSLESKWISNESSRYLTHKYRNKYQSIMVGINTVLNDNPLLTCRLNQE
                     KVSHPTRIVIDTHLKLPLNSNLVKDKTSKTIVFTCCKESKKLSMLKENNVETIISPSK
                     NNLVDLEFVMYKLGELNIDSVLVEGGATLNDSLFRNRLVDKVKLFLSPKIIGGKDAPT
                     FVSGEGINHLSDSTQLAINNVTLIDGDILIESDVLN"
     gene            1559..2224
                     /locus_tag="L3M60_003116"
     CDS             1559..2224
                     /locus_tag="L3M60_003116"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBN6227748.1"
                     /translation="MFTGIVEEVGILRKITANGKSGKVTILSNKILDGTNLGDSIAVN
                     GVCLTVSNLGKNEFTADVMMETIRSTNLGLLNANDKVNLERAMSLSSRFGGHIVTGHV
                     DGKGTICKFEKDENAVLVSIRPDKKLLSSMILKGSVAIDGVSLTISYLDDEIFKVSII
                     PHTKINTTLLAKNVGDFVNLESDVIGKYVNNFMVNNYKELNSNSSNHKSNIDKDFLFK
                     NGF"
     gene            2247..3440
                     /locus_tag="L3M60_003117"
     CDS             2247..3440
                     /locus_tag="L3M60_003117"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HBN6227749.1"
                     /translation="MFNTIEEAIEDIKNGKMVIVVDDEGRENEGDLLMAAEMATPESI
                     NFMATYGRGLICLPATEKKFKSLNIPLMVRENTDTFQTAFTVTIDGADTLTGISAYER
                     AETVKLFCDENSTSKDFKTPGHIFPLIAKTGGVLVRDGHTEASVDLARLAGFKEIGLI
                     CEIMKDDGTMARVDDLMIFKEKHNLKIITIKDLIEYRKINETTIEKVSSAFLPTKYGN
                     FEIIGYRDTYSNEEHIALTYGNINGENTLVRLHSECLTGDVFHSLKCDCGLQLESSMK
                     KIVENGSGVLIYMKQEGRGIGLLNKIKAYKLQEEGYDTVEANLMLGFEEDMRDFYMAA
                     QILKNLNIKSINLLSNNPDKINQLEKYGIKIENRVPINEEINDFNKLYLKTKKDKMGH
                     LLDII"
     gene            3546..4007
                     /locus_tag="L3M60_003118"
     CDS             3546..4007
                     /locus_tag="L3M60_003118"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBN6227750.1"
                     /translation="MIYEGKLIGKDLKIGIINSRFNEFITSKLLSGAEDCLLRHDVST
                     KNIEIVWVPGAFEIPLVAQKMAKSGKYDAIICLGCVIRGATSHYDYVCSEVSKGIAKV
                     SLDNELPVIFGIVTTENIEQAIERAGTKAGNKGYDCAMNALEMANLFKSLN"
     gene            4409..4837
                     /locus_tag="L3M60_003119"
     CDS             4409..4837
                     /locus_tag="L3M60_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227751.1"
                     /translation="MKTYSIQDLTLDKRNLIKKYSLTLNEDLIWEFKHSKYHTIKYFT
                     HKFAVKESVLALLFNIHKLCYAKIKYFESNFSKYEPYKYSYQHGFVKCEMYDMNFILH
                     KYSNIMIDLRNLQEIKTIEEFRLFCDYLESFEDNLQDLII"
     gene            complement(5047..6498)
                     /locus_tag="L3M60_003120"
     CDS             complement(5047..6498)
                     /locus_tag="L3M60_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBN6227752.1"
                     /translation="MDFFNGILTSINNAVVFVNNYLWSYILIIMLVVIGIYFTIKTNF
                     VQFRYFIEMFRLLGDGTANKNAKKEGKISSFQAFCISTSSRVGTGNIAGIAIAVVAGG
                     PGAVFWMWLIALIGSASSFVESTLAQIYKVKDGQAFRGGPAYYMEQGLNKKWMGILFS
                     ILITICYGFVFNAVQANTVSLAFSNAFGISKMAMGIGLGILTAIVIFGGVHRVAKVSE
                     IIVPVFAGLYILVALAIVVMNITEIPSVIALIVESAFDFKGMAIGTFMGVVMTGVKRG
                     LFSNEAGMGSAPNAAATAHVTHPVKQGLIQSLGVFTDTIIICSCTAFMVLLYSGYSSS
                     GATGIELAQEALTHHIGPVGNIFIAVCIFLFAFSSIVGNYYYGESNMEFMSGSKTKLN
                     VFRVFVVGMVLFGSLTQVDVVWNLADLFMALMAIINLIAIALLGKYAFIALKDYTSQK
                     KSGIKDPVFIADEIEGLENVSEWHRELPEEHLG"
BASE COUNT         2581 a          879 c          958 g         2262 t
ORIGIN      
        1 acttctttcc tgattatgtt caaattttta tttttatatt cacaatcgtg gtatttcaaa
       61 gtttatagac attctatatt atatattaac atatattttt aaaaaaaata gtttgcaaag
      121 taaaataatt tatgatacat tctaaatata aatttaatac ttaatcttcg gggtagggtg
      181 aaattcccaa tcggcggtat agcccgcgag ccaatgtaaa acttggttga tttggttaaa
      241 ttccaaagcc gacagtaaag tctggatgga agaagatatt taatgagtgt ttttgtagag
      301 caattttgtt gtacatttat taaacattta ttctagatta ttctaaaccc tgagttaatt
      361 ctcggggttt tttgtttaaa aattatccaa ttaaacagga ggtgtacata ttgtgaattt
      421 tgtgaatcaa aaagaaaaag atatttatta tatgaaaaaa gctattgaat tggctaaaaa
      481 tggagaaggc tttgtaaatc caaatccatt agttggatgt gtaattgtta aagatgatta
      541 cataataggt aaaggatatc atgagaaatt tggctctaat catgctgaag taaatgctat
      601 aaacagtgct aaacaaagtc taaaagactc tactctttat gtaaatttag aaccatgttc
      661 tcattatgga aaaacgcctc cttgtgttga taaaattatt caaaataaga ttaaaagagt
      721 tgtcatatct acattagacc caaatccact tgtttgtggt aatggagtaa aaaaattaag
      781 agataataat attgatgtta ctgtaggaac attagaagaa gaagcaagag atttgaatga
      841 agtatttttt tattatataa aaaacaaaag acctctttgc attgtaaaat cagcagtaag
      901 tctagatggt aaaattgcta caaaatcact agaatcaaag tggatatcta atgaatcatc
      961 aagataccta actcataaat acagaaataa ataccaaagt ataatggttg gcataaatac
     1021 agttttgaac gataatccct tactaacttg tagacttaat caagaaaaag tatctcatcc
     1081 tacaagaata gtaatagata cacacctaaa gttgccactt aatagtaatt tggttaaaga
     1141 taaaaccagt aaaactatag ttttcacatg ttgcaaagaa agtaaaaaat tatctatgtt
     1201 aaaagaaaat aatgttgaaa caataatttc tccttcaaaa aataacttag tagatttgga
     1261 atttgtaatg tataaattag gagaattaaa cattgattct gttctagttg aaggtggtgc
     1321 tacacttaat gacagtctct ttagaaatag actagttgat aaggttaaat tatttctatc
     1381 ccccaagatt ataggtggta aagatgcacc tacattcgtt tctggagaag gaataaatca
     1441 tttgtcagat tcaacacagc tagcaatcaa taatgtaact ttgatagatg gagatattct
     1501 tatagaatca gatgttttaa attaaatttt tattaataca tgtatatagg aggtcaggat
     1561 gtttactggc atagtagaag aagttggaat acttagaaaa attactgcta atggtaagag
     1621 tggaaaagta accatactat caaataaaat attagatggc acaaatcttg gagatagtat
     1681 agctgtaaat ggagtttgtc taactgtttc aaatctcgga aaaaatgaat ttacagctga
     1741 tgttatgatg gagacaattc gttccacaaa tttaggttta ttaaatgcaa atgacaaggt
     1801 aaatcttgaa agagctatga gtctttcttc gagatttggt ggtcatatag taacaggtca
     1861 tgttgatggt aaaggaacta tctgtaaatt tgaaaaagat gaaaatgcag ttttagtgag
     1921 tatacgacct gataaaaaac ttttatcatc aatgatactt aaaggttctg ttgctattga
     1981 tggtgtaagt ttaactatat catatttaga tgatgaaata tttaaggttt ctattatacc
     2041 tcatactaaa attaacacta ctctcttggc aaagaatgtt ggagatttcg ttaatttaga
     2101 aagtgatgta attgggaagt atgttaataa ttttatggtt aataactaca aggaattaaa
     2161 ttctaatagt agtaaccata aatcaaatat agataaagat tttttattta aaaatggttt
     2221 ttaaaaataa taaaggagtg aattacatgt ttaatactat tgaagaagct attgaagata
     2281 taaaaaatgg gaaaatggta atcgtagttg atgatgaagg tagagagaat gaaggagatt
     2341 tattgatggc agctgaaatg gctactcctg aatcaataaa ttttatggct acatatggta
     2401 gaggtctaat ttgtctacct gctactgaaa aaaaatttaa aagccttaat atacctctta
     2461 tggttagaga aaatacagat acattccaaa cagcctttac tgttactata gatggggcag
     2521 atactctgac tggtatatct gcatatgaaa gagctgaaac tgtaaaattg ttctgtgacg
     2581 aaaattctac aagtaaagat tttaaaactc ctggtcacat ctttccttta atcgcaaaaa
     2641 ctggtggagt tcttgttagg gatggacata cagaagcttc agtagacctt gctagacttg
     2701 caggttttaa agagattggt cttatatgtg aaataatgaa agatgatggt acaatggcaa
     2761 gagttgatga tttgatgatt ttcaaagaaa agcataattt aaaaatcatc actataaaag
     2821 accttataga ataccgcaaa ataaatgaaa ctactataga gaaagtatct agtgctttcc
     2881 ttccaacaaa atatggaaac tttgaaatca taggatatag agacacttat tcaaatgaag
     2941 aacatattgc tcttacttat ggaaatataa atggagaaaa tactctagtt agacttcatt
     3001 ctgaatgttt aacaggagat gtgtttcact ctttaaaatg tgattgtgga ttacaattag
     3061 aatcttctat gaagaaaatt gtagaaaatg gaagtggagt tttaatttat atgaagcaag
     3121 aaggtagagg tattggtctt ttaaataaaa ttaaagctta taaacttcaa gaagaaggtt
     3181 atgatactgt tgaagcaaat ttaatgcttg gatttgaaga agatatgaga gatttctata
     3241 tggctgctca aatattaaaa aatttaaaca taaaaagtat aaatctttta agtaataacc
     3301 ctgataaaat aaatcaatta gagaaatatg gaataaaaat cgaaaataga gtccctatta
     3361 atgaagaaat taatgacttt aataagctat atctaaaaac taagaaagat aaaatgggac
     3421 atcttttaga tattatataa gcgattatat gcggatattt ttctactaag taatttttta
     3481 taattaaact taaagatatt tataaattta aatctataac ataaataaaa ttggaggaaa
     3541 taaaaatgat atatgaagga aaacttattg gaaaagattt aaaaataggt ataataaatt
     3601 ctcgttttaa tgaatttata acatcaaaac ttttatcagg agctgaagat tgccttttaa
     3661 gacacgatgt ttctacaaaa aacatagaaa tagtttgggt acctggagct tttgagattc
     3721 ctcttgttgc tcaaaaaatg gctaaaagtg gtaagtatga tgctataatc tgtttaggat
     3781 gtgttataag aggagctact tctcattatg attatgtatg tagtgaagtc tctaaaggga
     3841 tagcaaaagt atctttagac aatgaattac cagttatatt tggaattgta actacagaaa
     3901 atattgaaca agctattgaa agagctggta caaaagctgg aaataaaggt tatgattgtg
     3961 ctatgaatgc attagaaatg gcaaatttat ttaagtctct aaattaaata atattattaa
     4021 ttaaatatta aaaattagtg taaaagatat aaaatactaa gtaccaaaga gagaagctat
     4081 atttcttctt ttgatactta gtatttttaa tataatcatg tatataaatc tcacaaaaca
     4141 atttacatcc ctttctaaaa ttatacatta tccatcaaat ctactttgaa aatcttacag
     4201 taattacaaa tataattcca aacataaaat accataaaag taagcatatt agtatttaag
     4261 ctattgtttt tatttataga acagactaat agtatactaa ttatctattt tattactttt
     4321 ttaatttacc ataaaatata attaagagta attaataata aaatactatt gcttaatact
     4381 tattagatat ttatgaggag aataaactat gaaaacatat tcaatacaag atttaacact
     4441 agataagcgt aatcttataa aaaaatattc tcttacttta aatgaggatt taatttggga
     4501 gtttaaacat agtaaatatc acactataaa atacttcaca cacaaatttg cagttaagga
     4561 atctgttctt gcactcttgt ttaatataca taaattgtgc tatgctaaaa taaaatattt
     4621 tgagtcgaat ttttcaaaat atgaacctta taaatatagt tatcaacatg gatttgtcaa
     4681 atgtgaaatg tatgatatga attttatctt acacaaatat agtaatataa tgatagattt
     4741 gagaaattta caagaaatta aaactataga agagtttaga ttattttgtg attacctcga
     4801 aagctttgaa gataatttac aggatttaat tatataatat ttttaagaag aaacccaaag
     4861 ataaaattta taatatacaa tctatcttca atgataaaaa acttttaata aagttaaatt
     4921 acataataaa aaaatattac tggaattaaa agttccagta atataaatta attcaatact
     4981 atattcatgt aaagtatatc catactctaa tctttcaaaa tgatttcata attttatact
     5041 atttttttat cctaaatgtt cctcaggtaa ttctctatgc cattctgata cattttctag
     5101 accttctatt tcatctgcaa taaatacagg gtcttttatt cctgattttt tttgacttgt
     5161 ataatctttt aaagcaataa atgcatattt tcctaaaagt gctattgcta ttaagtttat
     5221 tatcgccatc aatgccataa ataagtctgc aaggttccaa actacatcta cttgagtaag
     5281 tgaaccaaat aaaaccatac ctacaacaaa aactctaaat acattaagtt tagtcttact
     5341 tccactcata aactccatgt ttgattcacc ataatagtaa ttaccaacaa tagagctaaa
     5401 cgcaaacaag aatatacaaa ctgctataaa tatatttcca actggtccta tatgatgtgt
     5461 taaagcttct tgagctaatt caattccagt tgctccacta ctactataac ctgaatataa
     5521 taataccatg aatgctgtac aactacatat tattatagta tctgtaaaaa cacccaaaga
     5581 ttgaattaga ccttgtttta caggatgagt tacatgtgct gttgcagcag cattaggagc
     5641 actacccata cctgcttcat tagaaaatag acctctcttc actccagtca ttacaactcc
     5701 cataaatgta ccaattgcca ttcctttaaa atcaaatgca ctttccacta tcaacgctat
     5761 aacacttggt atctctgtta tattcattac aactatagct aaagcaacta atatatataa
     5821 tcctgcaaat acaggaacta ttatttcaga gacttttgca actctatgca ctccaccaaa
     5881 aataactatt gcagttaaaa ttccaagacc tattcccatg gccattttac ttattccaaa
     5941 tgcattacta aatgcaagtg aaacagtatt tgcttgaact gcattaaata caaaaccgta
     6001 acatatagtt ataagtattg agaataaaat acccatccat tttttattta gaccttgttc
     6061 catataatac gctggtccac ctctaaaagc ttgaccatct ttaactttat aaatttgagc
     6121 taatgtactc tcaacaaagc ttgatgcaga accaatcaat gctataagcc acatccaaaa
     6181 tacagcacca ggacctcctg ctacaactgc aattgctatt ccagctatat ttccagttcc
     6241 aactcttgaa gatgtactta tacaaaatgc ttggaatgaa gatatttttc cttccttttt
     6301 tgcattttta tttgctgtac catcacccag aagtctaaac atttctataa aatatctgaa
     6361 ttgtacaaaa tttgttttga tagtaaagta aataccaatt acaactaaca ttataattag
     6421 gatatacgac cataagtaat tgtttacaaa tactactgca ttattgatac tagttaaaat
     6481 accattgaaa aaatccatta gattaattac cccctattta gttttgtttt ctcaataaat
     6541 cttaaaatca aaaaaatgaa taattccttg ttaaaaattc attaatttaa ttataatatt
     6601 tataaaatca aattacaagg cttaaaatga ataatttttt taatatattg cagttttata
     6661 ttcagattaa accttttttt
//
LOCUS       DAFNNB010000169         6628 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0174, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000169 DAFNNB010000000
VERSION     DAFNNB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0174"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..745
                     /locus_tag="L3M60_003121"
     CDS             <1..745
                     /locus_tag="L3M60_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBN6227753.1"
                     /translation="FKFIEEKKKEGKIKNIGFSFHDSAELLDKVLSEHPEVDFVQLQI
                     NYLDWDNESIQSRKCYEVAQKHNKPVIVMEPVKGGTLAKIPEKAEKLLNDYNSDMSIP
                     SWAIRFAASKDNVMMVLSGMSNMEQLLDNTGYMQNFKPFIQEEYNIIDEAVGIINESI
                     LVPCTACQYCVEGCPKNIAIPNYFALYNAEKQALNTGFSTQMVYYNNYTKTYGKASDC
                     IECKQCEESCPQHIKIIDALKNVAEIFEK"
     gene            781..1194
                     /locus_tag="L3M60_003122"
     CDS             781..1194
                     /locus_tag="L3M60_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6227754.1"
                     /translation="MTISEVSKKYELSADTLRYYERIGLIPPVNRNKSGIRSFTEEDC
                     EWVNFIKCMRGAGLSIETLIEYVAMFQQGSSTIQARKELLIEQRNQLAKRMEEMQKTL
                     ERLNFKIDRYEEGIIEKEKVLKSSRNKKMEEVSYE"
     gene            1187..1726
                     /locus_tag="L3M60_003123"
     CDS             1187..1726
                     /locus_tag="L3M60_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HBN6227755.1"
                     /translation="MSKHVLIVSASPRKGGNSDLLCDQFTQGVLESKNQVEKIFLKDK
                     DINYCTGCGACYKKEASCPQKDDMGEILEKMIEADVIVLATPVYFYTMNAQMKTMIDR
                     TCSRYTEICDKDFYFIVSAADENIADMERTIEGFRGFTSCLNGSKEKGIVYGVGAWNI
                     GDIKGSEAMKQAYEMGKMA"
     gene            complement(1916..2401)
                     /locus_tag="L3M60_003124"
     CDS             complement(1916..2401)
                     /locus_tag="L3M60_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227756.1"
                     /translation="MNKSELTAETTEEMLEVLSGKDYDIACHLHELGKSLDCKIEPKT
                     GARSYKIVYSTKKPKRSLFTIECNEKKWRVKANLFHLNTYKDAVEKCSKTIKNSITKT
                     RTCKKCNSKCIGGSSFELDGKSYLTCIGSGHYFANMEEIDWKNLEKLITKENNIMQES
                     V"
     gene            3253..3630
                     /locus_tag="L3M60_003125"
     CDS             3253..3630
                     /locus_tag="L3M60_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003483933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBN6227757.1"
                     /translation="MSKVVIATEKAPGAIGPYSQAIQVGNIVSTSGQLPIDMKTGELV
                     SDPKEATKASLDNVKAILEQAGTSMDKIFKTTVFVKDLKDFNDINEVYATYFKENPPA
                     RSCVQVAQLPKDAVLEIEAMAVL"
     gene            4030..5838
                     /locus_tag="L3M60_003126"
     CDS             4030..5838
                     /locus_tag="L3M60_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6227758.1"
                     /translation="MLSKLKEFQQEMIKYTETVASVLDVDIEIVDDRLIRISGTGLYK
                     SKINESVVTEGFIYDNVIQTGQELVVLDICDNQLCIECSHYMKCLNKVIIAVPIKYNN
                     RTIGVIGAISTDKTKKVEISAKIDNYLKFVNHICDLISMKIEEHEVSKNSSRKMDMMI
                     EIIENVEKGVIILDINSKISYINNIALKKLDIDKNIIENIVNIVSVESSSNGHELLEI
                     DIDNKIYNINAKIIPVYPYINQYDKIIIFDKTYINHKGHVKVNSGWGNSDIESIIGNS
                     EAMLKVKERTKKLAKSNSTVLITGESGTGKELIARAIHAEGSRWNKPFIAINCAAIPE
                     NLLESELFGYIKGAFSGASSGGKVGKFELANEGVIFLDEIGDLSMPLQAKLLRVLQER
                     KFARIGSNKLIDLDIRVIAATNKNLLKLVNEGKFRDDLYYRLNVIPINLPPLRERKDD
                     IEAIMMKFASKYSLELGIQLNKIEENVMNMLINYNWPGNIRELENAVEYMMNLVGDDG
                     IIYKDMLPLDILNYYNINGNICKNKDINIIFEDDIVGGIVENQERILSIKELELTYIN
                     KLLNKYGRDTKTKKKIAKDLGIGLATLYRKLEEEQS"
     gene            6321..>6628
                     /locus_tag="L3M60_003127"
     CDS             6321..>6628
                     /locus_tag="L3M60_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003358107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HBN6227759.1"
                     /translation="MREIKWKLNNLPKSEEKEKGIEFLRDEEIAKAKAFHESFPQYEK
                     TPLVKLDNLAKSLGVSGIYVKDESYRFGLNAFKVLGGSYSMGRYLAQRLDTDISELGY
                     "
BASE COUNT         2682 a          697 c         1109 g         2140 t
ORIGIN      
        1 ctttaagttt attgaagaaa agaaaaagga aggaaaaata aagaatattg gtttttcttt
       61 tcatgatagt gcagaattat tggataaagt cttaagtgag catccagaag tagacttcgt
      121 gcaattacag attaattatt tagactggga taatgaaagc atacaatcta gaaaatgcta
      181 cgaggtggca cagaagcata ataaacctgt aattgttatg gagccagtaa aaggaggtac
      241 acttgcaaaa ataccagaaa aagcagagaa attattgaat gactacaatt cagatatgtc
      301 aattccatct tgggcaatac gttttgcagc aagtaaagat aatgttatga tggtcttaag
      361 tgggatgtca aatatggaac aattattaga caacacagga tatatgcaaa atttcaaacc
      421 attcatacaa gaggaatata atattataga tgaagcagta gggattatca acgaatcaat
      481 tttagttcct tgtactgcat gtcagtattg tgtagaaggc tgtccaaaaa atatcgctat
      541 tccaaactat tttgcattgt acaatgcaga aaaacaagct cttaatactg gattttcaac
      601 acagatggtt tactataata attatactaa aacttatgga aaggcttcag attgcattga
      661 atgtaaacaa tgtgaagaaa gttgcccaca acacattaaa attatagatg ctttaaaaaa
      721 tgtagcagaa atctttgaga aataattcaa tctatacaat atcaagaggg gaagatatat
      781 atgacaattt cagaagttag taaaaaatac gaactttcag cagatacttt acgatattat
      841 gaacgaattg gtttgatacc acctgtaaat agaaataaaa gtggaattag gtcttttaca
      901 gaagaagatt gtgagtgggt taatttcata aagtgtatga gaggtgcagg tctttctatt
      961 gagacattga ttgagtatgt agctatgttt caacaaggga gtagcactat acaagctaga
     1021 aaagaacttt taatagagca gagaaatcaa ctagcaaaaa gaatggaaga aatgcaaaaa
     1081 actttggaac gattaaattt taagattgat agatacgaag aaggaattat tgagaaagaa
     1141 aaagtattaa agagcagtag aaataagaaa atggaggaag tatcttatga gtaaacatgt
     1201 attgatagta tcagctagtc ctagaaaagg tggaaattct gatttattgt gtgaccagtt
     1261 tacacaaggg gtattagaat ctaaaaatca agtggagaag atatttttaa aagataaaga
     1321 tattaactat tgtactggat gtggtgcttg ttataaaaaa gaggcaagtt gcccacagaa
     1381 agatgatatg ggagaaatcc ttgaaaaaat gattgaggca gatgtgattg ttttagcaac
     1441 tccagtttat ttttatacta tgaatgcaca aatgaaaact atgattgata gaacttgttc
     1501 tagatatact gaaatatgtg ataaagattt ctattttatc gtttcagcag cggatgaaaa
     1561 tatagctgat atggaaagaa caatagaagg atttagagga tttacatcat gtttaaatgg
     1621 ttccaaggaa aaaggtatag tatatggtgt aggggcttgg aatataggtg atattaaggg
     1681 aagtgaggca atgaaacagg catatgaaat gggtaaaatg gcatagtata tttgtataaa
     1741 atagaatata tttttatcat ttcaacaata aaaaagaggc ttatctccat tttgagacag
     1801 cctctttatg ttatatcatt tatttttatt ctgtatcctt ttatatcttc tattttttga
     1861 tgtctattta agtatctgtt gttactcata tattttaata attaccctta aaaaactata
     1921 cagattcctg cataatatta ttctctttag taattaattt ttctaggttt ttccaatcaa
     1981 tttcttccat atttgcaaaa taatgaccac ttcctatgca agttaggtaa gactttccat
     2041 ctaattcaaa cgaagaacca ccaatacact ttgaattaca ttttttacaa gtacgagttt
     2101 tagttatgct atttttaata gttttagagc atttctccac agcatcctta tacgtattta
     2161 aatgaaaaag atttgcttta actctccatt tcttttcatt acattcaata gtaaataagc
     2221 tacgttttgg cttcttagtt gaatatacta ttttgtaaga acgagcacct gtctttggtt
     2281 caattttaca atctaatgat ttaccaagtt cgtgtaaatg acatgcaata tcataatctt
     2341 taccacttag tacttctaac atttcttctg ttgtctctgc tgttaattca cttttgttca
     2401 tattaaaaaa ttccccctat ataaataata atttcatata aaaataatta atatcagtat
     2461 attaaatgaa attattaaaa tcaatagaat tactataatc ttacattatt gggcatagaa
     2521 tgagagctaa tgatttatca gaaataagac ttaatagtat gaaaatttac ttagacgtgg
     2581 tacaattcta taaaagattt aaaactagga aaggagaaag ttgttgagat atagccaata
     2641 taacaattta tttatagatt gcaaatttat tatgagatta attagattgt taagtaagac
     2701 tattaaaagg tagtttttgg gattctcagc aacagtggta ggatattgat aattttttgg
     2761 ttactattat atttagctat tatattttcg ataggttcta tgataactag ggtaaataag
     2821 gtatgggtaa aggttttatt atttgtaata gtgggattgt caactatagt tctttcatat
     2881 gtaataactt gataatttta gagtaaaaaa caactttaat ctggaaattc tcaaaacaag
     2941 aattctcatg ttggtaaatt tctagatatg agaaaaaatt tgacattata aaaaagtaat
     3001 tttaaagcat ttttttacga aaaaattcag aagcaactat tttgaatgac ttcacaaaat
     3061 tttatatata aatgaagttt ggcatgttaa ttgctttaat aaaagttaac aagacacaaa
     3121 taaatataat catctaagta ttgattattt gaagttttag atttgaaatt taatctaaaa
     3181 gttcgtatta atattggtgt ttaggaaatt ttattttcct gtaaatataa cttaataata
     3241 ggaggtgaag tcatgtcaaa agtagtaata gctacagaga aagctccagg agcaattgga
     3301 ccatattctc aagcaattca agttggaaac atagtttcta catcaggtca acttccaata
     3361 gatatgaaga ctggcgagtt agtatctgac ccaaaagaag caacaaaagc ttctttagat
     3421 aatgtaaaag caatattaga acaagctggt acaagtatgg ataaaatatt taagactaca
     3481 gtattcgtaa aagatttaaa ggattttaat gatattaatg aagtgtatgc tacatatttt
     3541 aaagaaaatc caccagcaag gtcatgtgta caagttgctc aattaccaaa agatgcagtt
     3601 ttagaaattg aagcaatggc agtattataa gtattataac aataaaacca ataacaaaac
     3661 taatgaaaat taaaaactaa taaaaaaagt ctacaattaa aaaagtttat attaaaaaag
     3721 tctatataaa aatctatcta aatatttaac tattgagctt ttctcatata atgtatattt
     3781 ttatgttaaa aatatatttg ttatacgagg aaagcttttt ttattttatt attgtattgt
     3841 gatttatttt attgtctgca tattataatt aagattacta tataaaacaa actttttata
     3901 aattaatatc atgtgtttta gcgcaaatat ataaataagt aatgttttat atatatttta
     3961 aagagtgact atatgttaaa atatagctaa actgataatt tggtaaatta aattctaatg
     4021 ggggaaacta tgttaagtaa attaaaagaa tttcaacaag aaatgataaa atacactgaa
     4081 acagtagcga gtgtgttaga tgtagatatt gaaatagttg atgatagatt aataagaatt
     4141 agtggcacag gtctgtataa atcaaagata aatgaaagcg ttgttacaga aggctttatt
     4201 tacgacaatg ttatacagac aggtcaggaa ttagtcgtat tggatatatg tgataatcag
     4261 ctttgtatag aatgttccca ttacatgaaa tgcctaaata aagtaataat agcagttcca
     4321 ataaagtata ataatcgaac tataggagta attggtgcta ttagtacaga taaaactaaa
     4381 aaagtagaaa taagtgcaaa aatagataat tatttaaaat ttgtcaatca tatatgcgat
     4441 ttaatttcta tgaaaataga agaacatgaa gtgtcaaaaa atagcagtag aaaaatggat
     4501 atgatgatag aaatcattga aaatgttgaa aaaggcgtta taatactaga cataaatagc
     4561 aaaatatctt atataaacaa tatagctcta aaaaaattag atatagataa aaatataata
     4621 gaaaatatag taaatatagt atctgttgaa tcttcctcaa atggacatga attactagaa
     4681 attgatattg ataacaagat ttataatata aatgccaaaa taattccagt atatccatat
     4741 ataaatcaat acgacaaaat aataatattt gataagacat atatcaatca taaaggacat
     4801 gtaaaagtta atagtggctg gggaaatagt gatatagagt ctataattgg aaattcagaa
     4861 gcaatgttaa aagtaaaaga gagaactaag aaattagcaa agtcaaattc aactgtactt
     4921 ataactgggg aaagtggcac gggtaaagaa ctaattgcta gagctataca cgctgagggt
     4981 agtagatgga ataagccatt tattgcgata aactgcgctg ctattccaga aaatctcctt
     5041 gaaagtgagc tatttggcta tataaaaggt gcatttagtg gagctagtag tggaggtaaa
     5101 gtaggtaaat ttgaacttgc aaatgaaggt gttatatttc ttgatgaaat aggagattta
     5161 tcaatgcctt tacaagcaaa gttacttaga gttttgcaag agagaaaatt tgcgagaatt
     5221 gggtcaaata aacttataga tttagatata agagtgattg ctgctacaaa taaaaattta
     5281 ttgaaattag taaatgaagg taagtttagg gatgatttat actatagatt aaatgtaatt
     5341 cctataaatt tgccacctct tagggaaaga aaggatgata ttgaggcaat aatgatgaag
     5401 tttgcttcta aatattcttt agagttaggt attcagttaa ataaaataga agaaaatgta
     5461 atgaatatgt tgattaacta taattggccg ggaaatataa gggaactgga aaatgcagtt
     5521 gaatatatga tgaatttggt tggagatgat ggaataatat ataaagatat gcttccattg
     5581 gatattttaa attattataa tattaatgga aacatttgta aaaataagga tataaatata
     5641 atatttgaag atgacatagt tggaggaata gtagagaatc aagaaaggat actttcaatt
     5701 aaagaattag agttgactta cataaataag ctgttgaata agtatggaag agatacgaag
     5761 acgaagaaga aaatagcaaa agacttaggt ataggtttag ctacattata taggaagttg
     5821 gaagaagaac agtcgtaata taatagaaaa ttaaaaccat aaatctttat atattttaaa
     5881 actaaataaa atacacatat aattaaaata gaaataaaac ataaacttag aactaaataa
     5941 aatataatca aataaaatta cagaaggtta tagtcatata tttatagaaa ataaaagtag
     6001 taatatatct gataaaacaa tagacatata aaaaataaga gcataataaa ggaaaaatta
     6061 agacaaataa acgatttctc gaattgataa ttctcgtatt gataaaaatt tcccaatatg
     6121 agaatttttt cttttataaa tcttgttttt tgcactcaaa acttataaac tttaaagaaa
     6181 ttttataaaa gtatgtaatt ccaacataaa aaaattgttt ttagaataaa taatattggc
     6241 atgctaattg ctttactata agacacaaag ataaaaaatt aaaatgacat cgaaaaattt
     6301 gaaaggggaa tataaaataa atgagagaaa taaaatggaa attaaataac ttacctaaat
     6361 cagaggaaaa agaaaaagga attgaatttt taagagatga agagattgca aaggctaaag
     6421 cttttcatga gagctttcca caatacgaaa agactccttt agttaaatta gataacttag
     6481 caaaatcact aggcgtaagc ggaatatatg ttaaagacga atcttataga tttggtttaa
     6541 acgcatttaa agttttaggt ggttcttact ctatgggtag atacctagct caaagattag
     6601 atacagacat atctgagcta ggttatga
//
LOCUS       DAFNNB010000170         6614 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0175, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000170 DAFNNB010000000
VERSION     DAFNNB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0175"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..449
                     /locus_tag="L3M60_003128"
     CDS             <1..449
                     /locus_tag="L3M60_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HBN6227760.1"
                     /translation="KALNILSHADQSEKKLKEKLSSEFDENTIDKVIDFLKSYNLIND
                     SILAQKIVNTNVNLNKCGKNRIKQNLYNKGINRSTIDEAVSELDKDTEFENAMYLAKK
                     RYERVKKEDKKKIYQKISQHLSYKGFDYDIIKRVLNKLLNFDEYDI"
     gene            602..2605
                     /locus_tag="L3M60_003129"
     CDS             602..2605
                     /locus_tag="L3M60_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBN6227761.1"
                     /translation="MKSQKRFKILDIDIYLQPFESDIKKRMKHYEYMKSKILKNCKDF
                     SSFANAHLYYGFHKTNDGWVYREWAPNADSLSLIGDFNNWNRKSHPLRKIGSGNWEIF
                     IPGQNSLPHKSEVKVQVTTNGKTFDRIPLYIKRVIQKDFGGFNGQIWQPKTSFIWTDN
                     DFDLKNIISPLIYECHIGMSTESESIGTYNEFTEKILPKIKKAGYNTIQLMAIMEHPY
                     YASFGYQVSNFYAISSRFGTPEDLKNLINTAHSMGIAVLLDLVHSHAVKNTLEGINEF
                     DGSEHQFFHSGSKGNHPAWGTKLFNYGKPEVIHFLLSNIKFWLNEYHFDGFRFDGVTS
                     MLYHNHGLGVSFDSYEKYFSVNTDIEAITYLQFANELIKEIKPNSISIAEDMSGMPGM
                     CIPIKDGGIGFDYRLSMGVPDFWIKTISNLSDENWDLGKMWYELTTRRPGEKNIGYCE
                     SHDQALVGDKTIIFWLADKEMYWNMEINSNNPVINRAISLIKLIKLITFSLAGEGYLN
                     FMGNEFGHPEWIDFPREGNNWSYKYARRQWSLSEDNNLKYKQLLNFDKSMLELTKYSD
                     IFIQNSHELIHMDNDKKILVYIKGKYLFIFNFHPNLIQSVDISKFQNTNYKTILNTDI
                     VEFGGNTKKENLRDYDVYFSSTPPSSKIPIASRTAIVLLDNGI"
     gene            2779..3420
                     /locus_tag="L3M60_003130"
     CDS             2779..3420
                     /locus_tag="L3M60_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227762.1"
                     /translation="MGRKKEPDNTIQKILDISHKLFLEKGYEQTTIQDIVDNLDGLSR
                     GAIYHHFKSKDEIIKAITSRLYNDGFYGYDVKNDEGKTALEKLQYRLLHAYEQHNKQD
                     IFLMSSSLLRNPKYLATQFDEIINDIVPWIKALIEEGNQDNSMQVKNPEIAAQMFGFL
                     LNTWCIPTVYPGNPQQMVERFTLFGEQAAFMGISFMNDKIMCEYQKFVNKFSK"
     gene            3513..4196
                     /locus_tag="L3M60_003131"
     CDS             3513..4196
                     /locus_tag="L3M60_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227763.1"
                     /translation="MQKEVAMNKLIKDCVNFFNDCGFSYSICGGYALELFANMHIRPH
                     SDIDIWISDTDKEKSIQYMLNQGWDIYEPLGNYLLRPILDLNKQHIEQICVFAIKPDC
                     SFIELKKVEDDNYKMNIQCDEQLQFDFIELIYNPQKDGKFLFSQNQRIIRDMDKARLI
                     TSDNIPYMAPEVVLFCKSIYIDRVGYQKDFDVTVPLLSQEQRDWLVKALITAYPDGHA
                     WIDQLANQH"
     gene            complement(4373..4684)
                     /locus_tag="L3M60_003132"
     CDS             complement(4373..4684)
                     /locus_tag="L3M60_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBN6227764.1"
                     /translation="MKIITEIVEFNVEENLSKEEFIEIVNDLELKFHSIQSGFIDTEL
                     LYDEKSQKWIMIQHWESIEDLKSASSKMFKDSSTEKFRKSLKNQTVKMNIIPQIKSWK
                     L"
     gene            complement(4762..5580)
                     /locus_tag="L3M60_003133"
     CDS             complement(4762..5580)
                     /locus_tag="L3M60_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HBN6227765.1"
                     /translation="MLSVTRYSIKNIRLKPWVKFIWFLETKSNILLNNKLLPTDSIDI
                     ILNLSDVMEYKFENQDYTASNMHFNGIRDKHGFIVQHGNIRVIGISFYPFGLYPFLKI
                     PISEFNRQIVDLEAVSKLFAKKLEESLNPTKAIEKVVLCLEEVLLSILEEDLISNKSV
                     ELLNSFIYVNRYSNIKAFCDDTNINIKTLERTCLKYTGYTPKILKRIYRFKMASNQLI
                     YNYKDDELFDFIYENEYYDQAHFIKEFKKFSGTSPIKFIGENKTIKENIKYSYL"
     gene            complement(5680..6204)
                     /locus_tag="L3M60_003134"
     CDS             complement(5680..6204)
                     /locus_tag="L3M60_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBN6227766.1"
                     /translation="MEVTAKQKFTTKDMVETSLLIALVFIATKFINIRLPISINGGLV
                     HLGTAMLFISAIVFGSKKGALSGAIGMSLFDLISGWTLWAPFTFIVRGVMGYLLGKIV
                     WANGKNGNNFLINVIGICVSSIWMLFGYYVTEVILYGNFVVPLTSIPGNLMQVLIGLI
                     IALPISKVLKKCIK"
     gene            complement(6227..>6614)
                     /locus_tag="L3M60_003135"
     CDS             complement(6227..>6614)
                     /locus_tag="L3M60_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBN6227767.1"
                     /translation="ALDNILEEGIENVLNRHEKIAYSVRKSIEEYGLELFLEEGYSNT
                     VTAIKIPESIGALKLTDYMLKNYNTLVATSLNQYMDTILRIGHMGENANLNKIEHVLN
                     VLDKSLSALGFKENGTLLNLFNKYYF"
BASE COUNT         2451 a          932 c          937 g         2294 t
ORIGIN      
        1 ataaagcact aaatatttta tctcatgctg accagtctga aaaaaaatta aaagaaaaac
       61 tgtcatcaga atttgatgaa aatacaattg ataaggtcat agattttctt aaaagttata
      121 atctcataaa tgacagtata ttagctcaaa aaatagttaa cacaaatgtt aatctaaata
      181 aatgcggaaa aaacagaatt aagcagaatt tatataataa aggtatcaat agaagtacaa
      241 ttgatgaagc agtatctgaa ttagataagg acactgaatt tgaaaatgct atgtatctag
      301 caaaaaaaag gtatgaaaga gtgaagaagg aagataaaaa gaaaatatat caaaaaattt
      361 ctcaacatct ttcctataaa ggctttgatt atgatattat taaaagagtt ttaaacaaac
      421 ttttaaattt tgatgaatac gatatataag tatagatact agggaggtaa attatgaaat
      481 taatcattgg tggcttaagc tttttattgg gattatataa tatatatcaa ttatatagag
      541 aagatagtat gagataaata ttatttttta gatgtaatat ttactatgga ggagaaatac
      601 aatgaaaagt caaaaaagat ttaaaatttt agatattgac atatacctac aaccatttga
      661 atctgatata aaaaagcgaa tgaaacatta tgaatatatg aaatcaaaaa tattgaaaaa
      721 ctgtaaagat tttagttctt ttgcaaatgc tcatctttac tatggatttc ataagacaaa
      781 tgatggatgg gtctataggg aatgggctcc aaatgcagat agtttatcac taattggtga
      841 ctttaataat tggaatagaa aatctcatcc cctaagaaaa attggttctg gtaattggga
      901 aatatttatt cctggtcaaa atagcttacc tcacaaatca gaggtaaagg tacaagttac
      961 tacaaatgga aaaacatttg accgtatacc attatatata aaaagagtaa ttcaaaaaga
     1021 ttttggtgga tttaatggac aaatatggca accaaaaact tcatttatat ggactgacaa
     1081 tgattttgac ttaaaaaaca taatttctcc actaatatat gaatgtcata taggtatgtc
     1141 tactgaaagc gaatctattg gaacttataa tgaatttact gaaaaaatac ttcctaagat
     1201 aaaaaaagct ggatacaata ccattcagct tatggctatt atggaacatc catattatgc
     1261 atcttttgga taccaagttt caaactttta tgcaatctca tctagatttg gtactcctga
     1321 agacttaaaa aatctcataa atactgctca ttcaatgggg attgctgttt tattagattt
     1381 agttcattct catgctgtaa agaatacttt agaaggaata aatgagtttg atggaagtga
     1441 acatcaattt ttccactcag gttcaaaggg aaatcatcct gcttggggta caaaactttt
     1501 taactatggt aaacctgaag taatacattt tttgctttca aatataaaat tttggttaaa
     1561 tgagtaccat tttgatggct ttagatttga tggtgtaact tcaatgttat atcataacca
     1621 tggtcttgga gtttcatttg atagttatga aaaatatttt agtgtgaata cagatattga
     1681 agctataaca tatctccaat ttgcaaatga attaataaaa gaaattaaac ctaattcgat
     1741 aagtatcgca gaagatatga gtggaatgcc tggtatgtgt atccctataa aagatggcgg
     1801 aattggattt gactatagac tttcaatggg agtacctgat ttttggataa aaacaatctc
     1861 taacttatct gatgagaact gggatttagg caaaatgtgg tatgaactta ctactagacg
     1921 acctggagaa aaaaatatag gatattgtga atctcatgac caggcattag ttggcgataa
     1981 aacaattatt ttttggctag ctgacaagga aatgtactgg aatatggaaa taaactcaaa
     2041 taaccctgtt atcaatagag caatatctct tattaaatta attaaattaa taactttttc
     2101 tctagcaggt gaagggtatc taaattttat gggaaatgaa tttggacatc ctgagtggat
     2161 tgattttcca cgagaaggca acaattggag ctataagtat gctagaagac aatggagcct
     2221 ttcagaagat aacaacttaa agtataaaca attattaaat tttgataaat ctatgcttga
     2281 acttacaaaa tacagtgata tttttatcca aaatagtcat gaacttatac atatggataa
     2341 tgataaaaag attttggtgt acattaaggg caaatattta tttatattta atttccaccc
     2401 taatctaata caatcagttg atatttcaaa gtttcaaaat acaaactaca aaacgatatt
     2461 aaatactgat atagtagaat ttggaggaaa cacaaaaaaa gaaaacttaa gagattatga
     2521 tgtgtacttt agctcaactc caccttcttc taaaatacct attgcctctc gtacagcaat
     2581 agtgctttta gataatggta tttaaatatg agccaaaaat attaggaaac tgcaaatata
     2641 atccagtata aggcaaaaaa ataaaataat gaaagcatgg caatatacct gttcaacatg
     2701 ctaaaaatac acattgacaa atcattatgt atttgctaag ataaacatac caaatgattg
     2761 ttttgaggag tgagcttttt gggaagaaaa aaagaacctg ataatactat acaaaaaata
     2821 cttgatattt cacataagtt gttcttagaa aaagggtatg agcagacaac gattcaagat
     2881 attgtagaca atttagatgg cctttcaaga ggtgctattt atcatcactt taaatcaaaa
     2941 gacgaaataa ttaaggcaat cacaagtcgt ttgtataatg atggatttta tggatatgat
     3001 gtaaaaaatg atgaaggaaa gaccgcatta gaaaaattac aatataggtt gcttcatgct
     3061 tatgaacaac acaataaaca agacattttt ttaatgtcat catcactatt gcgtaatcct
     3121 aagtatttag ctacgcagtt tgatgagatt ataaatgata ttgtgccttg gataaaagca
     3181 ttgattgaag aaggaaatca agataattct atgcaagtta aaaatccaga gattgctgct
     3241 caaatgtttg gttttcttct aaacacatgg tgtattccaa cagtttatcc aggtaatcca
     3301 caacagatgg tagaacgttt tactttgttt ggtgagcaag ctgcttttat gggtatttct
     3361 tttatgaatg ataagataat gtgtgaatat caaaaatttg ttaataaatt ttctaaatag
     3421 taaatataaa actgccttaa tattggcagt tttatattta cacaaaacaa acatttagtt
     3481 ggtattttta aaaatttgat ttatttcggt acatgcaaaa ggaggttgct atgaacaaac
     3541 taattaaaga ttgtgtaaac ttttttaatg attgtggctt ttcatattca atatgtggtg
     3601 gatatgccct agaattattt gcgaatatgc atattcgccc acacagtgat attgacatat
     3661 ggatttctga cacagataaa gaaaagagca ttcagtatat gcttaatcaa ggatgggata
     3721 tatatgagcc cttgggtaat tatttattac gacctattct cgaccttaac aagcaacata
     3781 tagagcaaat ttgtgttttc gcgattaagc ctgattgctc atttattgaa ttaaaaaaag
     3841 tcgaggatga taactataaa atgaatatac agtgtgacga acagttacag ttcgatttta
     3901 ttgaattgat atataatcca cagaaagatg gaaaatttct tttttcccaa aatcaaagaa
     3961 ttataagaga tatggataag gccagactga taacatcaga taatattcca tatatggctc
     4021 ccgaagtggt tttgttttgc aaatcaatat acatagatag agttggttat caaaaagact
     4081 tcgatgtaac agtcccacta ttatcacaag agcaaaggga ttggcttgta aaggctttaa
     4141 taacagcata tccagatgga catgcatgga ttgaccaatt ggcaaatcaa cattaagcct
     4201 tttagctccc gaatatgaca ttatgataat ccacactcta acagtttgtt ggtttttcta
     4261 gtatataaca ctcatattaa aataaacact aaaagccaaa taacaattta aatacaaaat
     4321 gttatttggc taatttagtt tgatactatt taactatctt ttacattcag ttctataatt
     4381 tccaactttt aatttgtggt attatattca tcttaacagt ttgattcttt aaagattttc
     4441 taaatttctc agttgaagaa tctttaaaca ttttactaga agcactcttg aggtcttcta
     4501 tagattccca atgttgtatc ataatccact tttgagactt ttcatcatat aaaagctcag
     4561 tatcaataaa accggattga atagaatgaa attttaattc taagtcattt acaatttcta
     4621 taaactcttc ttttgacaaa ttttcttcca cattaaactc aactatttct gtaataattt
     4681 tcatatataa aacctccaat ttattttcct atatcatatc aaaaaataaa ttggaggtat
     4741 tgtaaaaaat cgacagtata attataaata gctgtacttt atattttctt taatcgtctt
     4801 attctcacca ataaatttaa ttggtgacgt ccctgaaaat tttttaaatt cttttataaa
     4861 gtgcgcttgg tcatagtact cattttcata aataaaatca aaaagttcat catctttata
     4921 attataaata agttgattac ttgccatttt aaatctgtaa attctcttta aaatcttagg
     4981 tgtataccct gtatatttta aacaggttct ttccaatgtt ttaatattta tatttgtatc
     5041 atcacaaaat gcttttatat tgctatatct attaacatat ataaatgaat ttaaaagctc
     5101 tacagattta tttgaaatta aatcttcttc taatatggat aataatactt cttctaaaca
     5161 taaaacaact ttttctattg cttttgttgg atttaatgac tcttctaatt tctttgcaaa
     5221 tagttttgat actgcttcta agtcaacaat ttgtctgtta aactctgaaa tcggtatctt
     5281 taaaaatgga tatagaccaa atggataaaa agaaatccct ataacacgta tatttccatg
     5341 ttgaacaata aatccatgtt tatcccttat tccattaaaa tgcatattac tagcagtata
     5401 gtcttgattt tcaaatttat attccataac atcagaaaga ttaagaataa tatctatact
     5461 gtctgtcgga agtaacttat tatttaacag tatatttgat tttgtctcaa gaaaccaaat
     5521 aaacttcacc cacggcttta aacgaatatt tttgattgaa tatcttgtaa ctgataacat
     5581 tgtgaactaa cctctatact aaaatttttt aattatgtac tccatcttaa tattatacag
     5641 tataatttat gctatcgtaa attttagttc actttatatt tattttatac atttttttaa
     5701 tactttagaa ataggaagcg ctattataag acctatcaat acttgcatta agttacccgg
     5761 tattgaagta agaggtacta caaaattacc ataaagtata acttctgtta cataatatcc
     5821 aaataacatc caaattgatg aaacacatat tcctattaca ttgattaaaa aattattccc
     5881 gttcttacca tttgcccata caatctttcc aagtaaataa cccataacgc ctctaactat
     5941 aaaggtaaat ggtgcccaca aagtccaccc agatattaaa tcaaaaagtg acataccaat
     6001 agctcctgat aaagcacctt ttttacttcc aaatactatt gctgatatga acaacattgc
     6061 agttcctaaa tgtacaagcc caccatttat agatattggc aatcttatat ttataaattt
     6121 agttgcaata aatactaatg ctattaatag tgaggtttcc accatgtctt tcgttgtaaa
     6181 tttttgctta gctgtaactt ccatactatc tccccctgtt tatttattaa aaataatact
     6241 tattaaataa gtttaacaaa gttccatttt ctttaaatcc taatgcactt aaacttttat
     6301 ccaaaacatt tagtacgtgc tctattttgt ttagatttgc gttttctccc atatgaccta
     6361 ttcttaaaat agtatccata tattgattta gtgatgtcgc aacaagtgta ttataatttt
     6421 taagcatgta atcagtaagc tttaaagctc ctatgctttc aggaattttt atagctgtaa
     6481 ctgtgttaga atatccttct tctaaaaata gttctaaacc atattcctca atagattttc
     6541 taacactata agctatcttt tcatgtctat ttaatacatt ttctattccc tcttctaata
     6601 tattatcaag tgct
//
LOCUS       DAFNNB010000171         6605 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0176, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000171 DAFNNB010000000
VERSION     DAFNNB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6605
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0176"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            415..1359
                     /locus_tag="L3M60_003136"
     CDS             415..1359
                     /locus_tag="L3M60_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HBN6227768.1"
                     /translation="MKFLRIEDEVIYSKKRYKLKREIDKLFLDEEYFQIEPQLFEEYD
                     EFITINNKIPEESMVKVVNGKVMVLRADITTSIIKSLVPRWEDGLKLKLFYNSSIYKN
                     KNTVGIKEIRQIGCEYLGESSVEADREVVKLALKILEKYNNKFILEVGSSKYIHGLLE
                     ELNLNKNCENQIKNLLYTKNIHELKVYIEELKIKCEVKELLSNILSLQGNLYNVIEKS
                     NKFYCNNKMKQALEELKQVNNLIEECNFLDKARFDLSMITMLDYYEGIMFRGYYPNSY
                     KEILSGGRYDSLTKEYGKEIPAIGFTLSVDELMKYVYK"
     gene            1381..2007
                     /locus_tag="L3M60_003137"
     CDS             1381..2007
                     /locus_tag="L3M60_003137"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBN6227769.1"
                     /translation="MDYLTIALAKGRIEGESFKKLKKMGLGNSIDTDTRKLIFKDEEN
                     KIIYIHVKPSDVVTYVEKGVADLGIAGKDTILENETDVYEIYDLGFGKCKFAVAGLKG
                     DSIYREDEYLKVATKYPNIAKKYFKEKGQKIEIIKLNGSVELAPIVGLSDVIVDIVET
                     GNTLKANGLEILEDICNISARIISNRASYRFKYEQIQNIIRLFEELDN"
     gene            2041..3090
                     /gene="hisC"
                     /locus_tag="L3M60_003138"
     CDS             2041..3090
                     /gene="hisC"
                     /locus_tag="L3M60_003138"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBN6227770.1"
                     /translation="MREKESIRELRGYEPNHVNCKVKLDANEGSKRLFKYLIKEIADS
                     NIDLNLYPEDSYSDLKESIIDYINISGVSKKNLLVGNGSSEIIDLIIHTFVDKDEVIL
                     SFSPSFSMYSIYSQINGSKFIGVESDENLVIDIDNVIEKVKENNPKIVIVCNPNNPTG
                     TILKRKDIKKLLDSTNSLVVLDEAYMDFGEESMLSDVFKYDNLIVLRTLSKAFGLAGI
                     RTGYMLSNSSLINSVEKVRPPYNLNSLSDFIATRALRNKDVVKAYIKEVKEERKILYE
                     EMIGMGIKAYKSQANFILFYSEIENLSQKLIDRGVLIRKFGGKLENYYRVTIGDKEEN
                     AMFIGAIRDILKKEK"
     gene            3093..3671
                     /gene="hisB"
                     /locus_tag="L3M60_003139"
     CDS             3093..3671
                     /gene="hisB"
                     /locus_tag="L3M60_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HBN6227771.1"
                     /translation="MRIWNVERNTLETQISVELNIDGSGKAEIDTGIGFLDHMLTLMA
                     FHGKFDLKVICKGDTCVDDHHSVEDIGIAIGESFKNALGDKKGIRRYSNIYIPMDESL
                     SMVSIDISNRPYLVFNAKFDIQMIGSMSTQCFKEFFRAFVNESRVTLHINLLYGENDH
                     HKIESIFKAFARALKEGSEIVSNEIASSKGVL"
     gene            3673..4278
                     /gene="hisH"
                     /locus_tag="L3M60_003140"
     CDS             3673..4278
                     /gene="hisH"
                     /locus_tag="L3M60_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBN6227772.1"
                     /translation="MNIIVDYGLGNIDSVSRGFRKAGIETKISSNIDEIKQADSLILP
                     GVGAFRDSISALEKLGLIPIIKEHVSNGKFMIGICLGMQLLYEKSYEYGEYEGLGLIK
                     GSIDKLDISLKVPHMGWNNLKFNKANDDILKYINEDDYVYFVHSYYANSSNEELVAFS
                     EYEKKIPAIVRKGNVYGIQFHPEKSGEVGLNILRAYGEMIK"
     gene            4275..4997
                     /gene="hisA"
                     /locus_tag="L3M60_003141"
     CDS             4275..4997
                     /gene="hisA"
                     /locus_tag="L3M60_003141"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBN6227773.1"
                     /translation="MIIFPAIDIKDNKCVRLTQGEFDKINVYYDNPLEVAYKWKNEGA
                     EYIHIVDLNGARSEFGVNTKIIDEIANNIDIPIQVGGGVRDKEKVKSLINAGVTRVIL
                     GSIAVENLNLVEELVNEYKEKIVVSIDAKDGKVAVRGWEIVSDVDSLTLCKQLEKIGV
                     QTIVYTDISKDGMLQGPNFNIYEKIAKETSLNVIASGGVTSIEDVKRLKAMNLYGAII
                     GKALYDKKIDFKEAQQLCLLGE"
     gene            4979..5737
                     /gene="hisF"
                     /locus_tag="L3M60_003142"
     CDS             4979..5737
                     /gene="hisF"
                     /locus_tag="L3M60_003142"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBN6227774.1"
                     /translation="MLTRRIIPCLDVRNGRVVKGKKFKDIVDVDSPEVLGKFYSDCGA
                     DELVFYDITASNEERTTSLEFVTKVAENISIPFCVGGGVNKLEDFTDILRKGADKVSI
                     NSSAVKNPELIREASLKFGAQCVVLSIDAKKNEEGSWSVYVKGGREKTNLDAIEWAVK
                     GVELGAGEIVVNSMDEDGMKNGYDIELLSKITSLVNVPVIASGGAGKMEDFYQAVNKS
                     NVDGILAASVFHFGEIKINDLKKYLKNMGVEVRL"
     gene            5765..6433
                     /locus_tag="L3M60_003143"
     CDS             5765..6433
                     /locus_tag="L3M60_003143"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBN6227775.1"
                     /translation="MNNKCNDIYTDEVDEFIRSIKFDDKGLVPVVVQEVVSKDVLMLA
                     YMNKEAIKKTLKDKVACYFSRSRQELWVKGETSGNTQKVVKMSYDCDVDTILLFVEQT
                     GVACHTGNYSCFYRDLFDDTAKMELEVQTNILKELYDLINERKNNPVEGSYTNYLFEK
                     GIDKILKKVGEESSEVIIASKNTDKSELIYEISDLVYHTLVLMIEKGVEIDEIKKELL
                     KRRK"
BASE COUNT         2694 a          543 c         1285 g         2083 t
ORIGIN      
        1 taggtgaaga ttaaattttt tattgtataa tcaaaagtta catataaaat attttattcg
       61 attttttaaa taactattgc caatttttga aaaatatatt attattaata gtaacaaaaa
      121 actgtaaatt tagaatatta ataaaaatta tttaggagga aattatcatg gtgagattaa
      181 atatagtaaa attaaataca aaattaaatc atcaccatca tcatagataa aagggattgt
      241 aatatgaaat taattcatag acacaagccc tgtttttagt gtaattggtc ttgtatctat
      301 gatgggaagt aattttaaga gaaaattata gccatatagg tataaagcct atatggcttt
      361 ttattgtttt aaatttgcaa atataagagt aaatttaatg aagagggtga gaatgtgaag
      421 tttttgagga ttgaagatga ggttatttat tctaaaaaaa gatataaatt aaaaagagaa
      481 atagataaac tttttttgga tgaagaatat tttcaaatag aaccacaatt atttgaagag
      541 tatgatgaat ttataactat aaataataaa attccagaag aatctatggt aaaggttgta
      601 aatggaaaag taatggtact tagagctgat ataactacaa gtataataaa aagtcttgta
      661 cctagatggg aagatggttt aaagcttaag cttttttata attcatcaat atataaaaat
      721 aaaaatacag ttggaataaa agagataaga caaataggtt gcgagtattt aggagaatct
      781 tctgtagaag cagatagaga agtagtgaaa ttagctctta agatacttga aaaatacaat
      841 aataaattta ttttagaggt tgggagtagc aagtatattc atggtttgtt agaagaatta
      901 aatttaaata agaattgtga aaatcaaatt aaaaatttac tatatactaa gaacattcac
      961 gaattaaaag tatatattga agagctaaaa attaagtgtg aagtaaaaga gttattatca
     1021 aatattttaa gtttacaagg aaatttatac aacgttattg aaaaatcaaa caagttctat
     1081 tgtaataata agatgaagca agctcttgaa gaattaaagc aagtgaataa tttaattgag
     1141 gaatgtaatt ttttagataa ggccagattt gacttatcaa tgattacgat gcttgactat
     1201 tatgaaggaa ttatgtttag aggatactat ccaaattcat ataaagaaat tcttagtggt
     1261 ggaagatacg attctttaac taaagaatat ggaaaagaaa ttccagccat tggatttaca
     1321 ctaagtgttg atgaattaat gaagtatgta tataagtaaa gctgagaagg aggaattgat
     1381 ttggattatt tgactatagc actagcaaaa ggacgaattg aaggggaatc atttaaaaaa
     1441 ttaaaaaaaa tgggtttagg aaatagtata gacacagata caagaaaact aatttttaaa
     1501 gacgaagaaa ataaaatcat atatatacat gtaaagccat cagatgtagt tacatatgtt
     1561 gaaaaaggag ttgctgacct tggtatagca ggtaaggata ctattttaga gaatgagacg
     1621 gatgtatatg aaatatatga tttaggtttt ggaaaatgta aatttgctgt ggcaggatta
     1681 aagggagata gcatatatag agaagatgaa tatctaaaag tggcaacaaa gtatcccaat
     1741 atagcgaaaa agtattttaa agagaaaggt caaaaaatag aaataataaa attaaatggt
     1801 tctgtagaac tagctccaat agtagggttg tcagatgtaa ttgtagatat tgttgagact
     1861 ggcaatacgc ttaaagcaaa tggtcttgaa atattagaag atatatgtaa tataagcgct
     1921 agaattatat caaatagagc tagctataga tttaaatacg aacaaattca gaatataatt
     1981 cgtttgtttg aagaacttga taattaaaat ataatggaac tttacaagga ggggtttaag
     2041 ttgagagaaa aagaaagtat aagagagtta agaggatatg aaccaaatca cgtaaactgt
     2101 aaagtaaaac ttgatgcaaa tgaaggaagt aagaggttgt ttaaatacct tattaaagag
     2161 attgcagata gtaatataga tttaaattta tatcctgaag attcttatag tgatttgaaa
     2221 gaatctataa tagattacat aaatatttct ggagtaagta agaaaaatct tctagtagga
     2281 aacggttcaa gtgaaataat tgatttaata atacatactt ttgtagataa agatgaagta
     2341 atactgagtt tctctccaag ttttagtatg tattctatat atagtcaaat aaatggttct
     2401 aaatttatag gtgttgaaag tgatgaaaat ttagttatag atatagataa tgtaattgaa
     2461 aaagtaaagg aaaataatcc taaaatagta attgtatgca atcctaataa tccaactgga
     2521 acaatactaa agaggaaaga tataaaaaag cttttagatt caactaatag tctagtggtt
     2581 ttagatgaag catatatgga ttttggagaa gaaagtatgt taagtgatgt ttttaagtac
     2641 gataatctaa tagttcttag aactttatca aaggcgtttg gattggctgg aataagaaca
     2701 ggatatatgt tatctaatag cagtttaata aattctgttg aaaaagtaag acctccatat
     2761 aatttaaatt cattgtcaga ctttattgct acaagagcac ttagaaataa agatgtggta
     2821 aaggcttata taaaggaagt aaaagaggaa agaaaaattt tatatgaaga gatgattggt
     2881 atgggaatta aggcatataa atcacaagcg aattttatat tgttttactc tgaaatcgaa
     2941 aatttatcac aaaaacttat tgatagagga gttttaataa gaaaatttgg tggaaagtta
     3001 gaaaattatt atagggttac gattggtgat aaagaagaaa atgctatgtt tattggagct
     3061 ataagagata ttttgaaaaa ggagaaatag gtatgagaat ttggaatgta gaaagaaata
     3121 ctcttgaaac acagatttca gtagaattaa atattgatgg ctctggtaaa gctgaaatag
     3181 atactggaat aggattttta gaccatatgc ttaccctaat ggcatttcat ggcaagttcg
     3241 atttaaaagt gatttgtaag ggtgatacat gtgtagacga ccatcatagt gtagaagata
     3301 taggtatagc cataggtgaa tcatttaaaa atgctctagg tgataaaaaa ggaataagaa
     3361 gatattctaa tatctatata cctatggatg aatctctatc aatggtatca atagatataa
     3421 gcaacagacc ttatcttgta tttaatgcta aatttgatat acaaatgatt ggtagtatga
     3481 gcactcaatg ctttaaagaa ttttttagag cttttgtaaa tgaatctaga gtaacattgc
     3541 atatcaatct cttatatggg gaaaatgacc atcataagat agaaagtatt tttaaggcat
     3601 ttgcaagagc tttaaaagaa ggtagtgaaa tagtttctaa tgagatagca tcttcaaagg
     3661 gggttttgta gaatgaatat aatagtagat tatggtcttg gaaatataga ttctgtgtct
     3721 agaggtttca gaaaagctgg gatagaaact aaaatttcta gtaatataga tgaaataaaa
     3781 caggctgatt cacttatact tccaggtgtt ggagctttta gagattctat aagtgcctta
     3841 gaaaagctag gattgatacc aataataaaa gagcatgttt ctaatgggaa atttatgata
     3901 ggtatatgtc taggaatgca attactttat gaaaaaagtt atgagtatgg agaatatgaa
     3961 gggttaggac ttataaaagg aagtattgat aaactggata taagtttaaa agtaccacat
     4021 atgggatgga ataatttaaa atttaataag gcaaatgatg atatacttaa atatataaac
     4081 gaagatgatt atgtatactt tgttcactca tattatgcaa attcatcaaa tgaagaacta
     4141 gttgcatttt cagaatatga gaagaaaatt cctgctattg ttagaaaagg caatgtatat
     4201 ggtatacagt ttcaccctga aaaaagtgga gaagtaggtt taaatatatt aagagcatat
     4261 ggggagatga taaaatgata atatttccag caatcgatat aaaagataat aagtgtgtga
     4321 gactcacaca aggagaattt gacaaaataa atgtatatta tgataatccg ttagaagtag
     4381 catataaatg gaaaaatgaa ggagcagaat atatacatat agtagattta aatggtgcta
     4441 gaagtgaatt tggtgtaaat acaaaaataa ttgatgaaat agcaaacaat atagatatac
     4501 ctattcaagt tggtggtgga gttagagata aggaaaaagt taaaagttta ataaatgctg
     4561 gtgttactag agtaatatta ggtagtatag cggttgaaaa tttgaatttg gttgaagaat
     4621 tggtcaatga gtataaagag aaaatagtgg tttctataga tgctaaagat ggaaaggtag
     4681 cagttagagg gtgggaaatt gtaagcgatg tagactcatt aacattgtgt aaacaacttg
     4741 aaaagatagg tgtacaaact attgtttata cagatatatc aaaagatggt atgttgcaag
     4801 gaccaaattt taatatatat gaaaaaatag caaaggaaac atcattaaat gttattgctt
     4861 ctggaggcgt aacttctata gaagacgtaa aaagactaaa agctatgaat ttatatggtg
     4921 caataatagg taaagcgctt tatgataaaa agatagactt taaggaggca caacaattat
     4981 gcttactagg agaataatac cttgtctaga tgtgagaaat ggaagagttg taaaaggaaa
     5041 aaaatttaaa gatatagtag atgttgatag tccagaggtg cttggaaaat tttatagtga
     5101 ttgtggtgca gatgaactcg tattctatga tataactgca tcaaatgaag agagaactac
     5161 gtctttagaa tttgtgacaa aagttgcaga aaatataagt ataccatttt gtgttggtgg
     5221 aggagtaaat aaattagaag atttcactga tattttaaga aaaggtgctg ataaagtttc
     5281 tataaattca tcagcagtca aaaatcctga attaataagg gaagcttcat taaaatttgg
     5341 agcacaatgt gttgttttat cgatagatgc aaagaaaaat gaagagggtt catggagcgt
     5401 atatgtaaaa ggtggaagag aaaaaactaa tctggatgca attgaatggg ctgttaaagg
     5461 tgtagaactt ggagcaggag aaatcgttgt aaatagtatg gacgaagatg gaatgaaaaa
     5521 tggatatgat attgaattat tatcaaagat aacttcttta gtaaatgtac ctgttatagc
     5581 atctggaggt gctggaaaaa tggaagattt ctatcaggcg gttaataaat ctaatgtaga
     5641 tggaatttta gcagcatctg tttttcattt tggtgagata aaaataaatg atttgaagaa
     5701 atatttaaaa aacatgggtg tagaagtaag attataagtt aaatattgaa aggagattag
     5761 aaatatgaat aataaatgta acgatatata cactgatgaa gtagatgaat ttataagaag
     5821 tataaagttt gatgataaag gattagttcc tgttgttgtt caagaggtag tgagtaaaga
     5881 tgtactgatg ttagcatata tgaataaaga ggctataaag aaaacattaa aagataaagt
     5941 ggcttgctat tttagtagaa gtagacaaga actttgggtt aaaggtgaga catctggtaa
     6001 tactcaaaaa gtagttaaga tgtcttatga ttgtgatgta gatacgatac tactttttgt
     6061 tgaacaaact ggtgtagctt gtcatacagg aaattattct tgtttttaca gagacttgtt
     6121 tgatgatact gctaaaatgg aacttgaagt acaaacaaat atattgaaag aattatatga
     6181 tttaattaat gaaagaaaaa ataatccagt tgaaggttct tatactaatt atctttttga
     6241 aaaaggaata gataagattc ttaaaaaagt aggtgaagag agtagtgaag taattatagc
     6301 gtcaaaaaat accgataaaa gtgaacttat ttacgagatt agtgatttag tatatcatac
     6361 attggtttta atgatagaaa aaggtgttga aattgatgaa ataaaaaaag aattacttaa
     6421 aagaagaaaa taggatagaa tagattaatg ctaataagaa aatataccgc ttttaataaa
     6481 aagtggtata tttttttatt ttatattttc gatttataga ataatatttg aattacttga
     6541 taaaattaac tatatatata atttgttgat aaaataattg tagtacatta tttattttag
     6601 aaaaa
//
LOCUS       DAFNNB010000172         6404 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0177, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000172 DAFNNB010000000
VERSION     DAFNNB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0177"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            82..4335
                     /locus_tag="L3M60_003144"
     CDS             82..4335
                     /locus_tag="L3M60_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HBN6227776.1"
                     /translation="MDELFHIGIDVGSTTVKVVVLNNSNNIVHKEYRRHYSDVKKSVK
                     EVLNGIYERFGNINTTIIITGSGGIGISKKLGVKFVQEVISSTKSIEYFHPETDVVIE
                     LGGEDAKITYLSGGIDQRMNGICAGGTGAFIDQMASLLKTDASGLNELAKGYNVIYPI
                     ASRCGVFAKTDIQPLINDGAKQTDIAMSIFNAVVVQTVSVLSCGRKIEGNVAFLGGPL
                     YFLSELREAFKRVLDLKDENIIFPENAQLYIAIGAGLLSVDEYSIQLKSLIEKLDSIK
                     NIEDGEVNLLEPLFKDKNEYEEFSRRHEKEKINYVDINSIKSNCYLGIDAGSTTTKAA
                     LIDEDGRLVYSYYNSNEGNPLKTTIKVINDIYDILPKDVKILSSTVTGYGEGLIKKAL
                     KIDNGEIETIAHYKAAKFFNKDVDFILDIGGQDMKCLKIKDGVIDNIILNEACSSGCG
                     SFLETFASSLSMTIEEFAHEGIYSQNPVDLGSRCTVFMNSRVKQAQKEGASVGDISAG
                     LSYSVIKNALFKVIKIRDLNEIGNNIVVQGGTFYNDLVLRSFEKLIGKNVIRPNISGI
                     MGAFGSALIAKEKYESGYKTSLLSKEELNNIKLEASVTRCKGCSNHCLLTINKFSDNE
                     IFISGNRCEKGEAIYGDKKIGLEKKKPINLFKYKYNRIFRYKPLEKEEAKNGEIGIPR
                     VLNMYEDYPFWFTFFNAMGFRVVLSDRSSKQLYETGITSIASETVCYPGKLVHGHIEN
                     LIAKGIKNIFYPSVTNENKEDSNADNYYNCPVVISYSEVIKNNVENIRNKNINYINPF
                     ISLNDKEKLKKRLYDELSRHFSDIKITKEEINNAVDKATDEQNLFKMEIQAAGERALK
                     EIKERNMKGIVLCGRPYHIDPEINHGMPELINSLDMAVLTEDSICHLANIQRPLRVVD
                     QWVYHSRLYKAASFVRDKNYLELVQLNSFGCGLDAVTTDQVQEILNEKSKIYTIIKID
                     EGNNLGAAKIRMRSLKAAMLERESKNIDVKNIKVNRIQYAKNNHITNKHTILAPQMSP
                     IHFQFLEKAVNLSGYNIEILKDTDSSVIEEGLRYVNNDACYPAIIVVGQLITALKSGK
                     YDLNNTSVTITQTGGGCRATNYIGFLRKAMYDAGFKDVPVIALSVNGIEDSGIMDNIS
                     LKLINRLFMSVVYGDLLMKVLYRVRPYEKVTGSANALYEKWVDICKTSLVKAKISVFT
                     RNIKDIVKEFDNLEILDIKKPKVGLVGEILVKFHPIANNNLVDILEREGAEAVVPDLT
                     NFFLSCAFNTIYKHTHLEGSRKSRMIGEVFIYITGIYQRVYKKALDKSERFYAPANIK
                     NVAKSTEPVVSLGNQTGEGWLLTGEMVELLNEGVENIICMQPFGCLPNHIIGKGSIKE
                     LKRLYKNANIIPIDYDPSASEVNQLNRIKLMLSKAFKNI"
     gene            4484..4978
                     /locus_tag="L3M60_003145"
     CDS             4484..4978
                     /locus_tag="L3M60_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBN6227777.1"
                     /translation="MSLVYDVYKSKLGNLYILSEDDFIVSVYIGDEKFNNLKNEYIKK
                     GIDIKRSKEDLKEAISQIDDYFNGKRKVFNLNIRIEGTEFQKSVYREMLKIPYGETLC
                     YSDIARNINNPKSVRAIGQASKSNKIPIIIPCHRVVGKKNIGGYMGNHSDLKEILLNL
                     ERQS"
     gene            5094..5471
                     /locus_tag="L3M60_003146"
     CDS             5094..5471
                     /locus_tag="L3M60_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HBN6227778.1"
                     /translation="MCDKVIASKYHEKGFNCAESVIKAYNEEFNTDIPVCLGSGLGSG
                     CGVASLCGAVNASNIIIGYVKGRNHEDESTRAKVYAKDLTTTVRKEYGSELCIDLKKD
                     LVACREIMDFAYDSLKETLKKEL"
     gene            5678..6118
                     /locus_tag="L3M60_003147"
     CDS             5678..6118
                     /locus_tag="L3M60_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufBD protein"
                     /protein_id="HBN6227779.1"
                     /translation="MDNINLLINRLYSKNHNEAYKTFLFLENESLKSNITYCFFDSFL
                     EMINNENSYIRARGLLLISANAQWDIDNKIEINIDSILSHIVDKKPFVSRMFIKSIPN
                     ITKYKKNLIRRIKMELSNADISIYNNNMKPLVEKDINDTLSNLS"
BASE COUNT         2636 a          629 c         1119 g         2020 t
ORIGIN      
        1 taaacattat aaataactta cttaaaataa agccatagaa cttacaaaaa ttaaaaaagc
       61 atataaatat ggaggggtac aatggatgaa ttatttcata taggaattga tgtaggttct
      121 acaacggtga aggttgtggt acttaataat agtaataata tagttcataa agaatataga
      181 agacactatt ctgatgtgaa aaaatcagtt aaagaagtac taaatggtat atatgaaaga
      241 tttggaaaca taaatactac cattataata actggttcag gtggaattgg aatatcaaaa
      301 aaattaggcg taaaatttgt tcaggaagtt atatctagta caaaatcaat agagtatttc
      361 catcctgaaa ctgatgttgt catagaactt ggaggagaag atgcaaaaat aacatatcta
      421 agtggaggaa tagaccaaag aatgaatgga atatgtgcag gtggcactgg agcattcata
      481 gaccaaatgg catcactttt aaagactgat gccagtggat taaatgaact tgctaaaggg
      541 tataatgtta tatatcctat agcttcaaga tgtggtgttt ttgctaaaac agatattcaa
      601 cctcttataa atgatggagc taagcaaact gatatagcaa tgagtatttt caatgcagtt
      661 gtcgtacaaa ctgtgagtgt tctctcttgt ggaagaaaaa tagaaggaaa tgttgcgttt
      721 ttaggtggtc cattatattt tttatctgaa cttagagaag catttaaaag agttttggat
      781 ttaaaagatg aaaatataat cttcccagaa aatgcacagc tttatattgc tattggagca
      841 ggattattat cagtagatga gtatagtata caactaaaat ctctaataga aaaattggat
      901 agtataaaga atattgaaga tggagaagta aatttattag aacctttgtt taaagacaaa
      961 aatgagtatg aagaattttc aagaagacat gaaaaggaaa aaataaatta tgtggacata
     1021 aattcaataa agagtaattg ttatttggga attgatgcag gctcaactac aactaaagca
     1081 gcacttattg atgaagatgg aagacttgta tattcttatt acaatagcaa tgaaggaaat
     1141 ccattaaaaa caaccattaa agtcataaat gatatatatg acatattacc aaaagatgtt
     1201 aaaatattga gttcaactgt aactggatac ggagaaggtc ttattaaaaa ggctcttaaa
     1261 atcgataatg gagagataga gactatagca cattacaaag ctgctaaatt tttcaataaa
     1321 gatgtagatt ttatacttga tattggtggt caagatatga agtgcctaaa aataaaagat
     1381 ggagtaattg ataatataat attaaatgag gcttgttcat caggttgtgg ctcattttta
     1441 gagacatttg caagttcttt atctatgaca atagaagaat ttgcacatga aggtatttat
     1501 tctcaaaatc ctgtagattt aggttcaaga tgtactgtat ttatgaactc tagagtaaaa
     1561 caagctcaaa aagaaggtgc aagtgttgga gatatatcag cggggctatc ttattcagta
     1621 ataaaaaatg ccttatttaa agtaataaag attagagatt taaatgagat tggtaataat
     1681 attgttgtac aaggtggaac cttttataat gatttagtgc ttagaagctt tgaaaaacta
     1741 attgggaaaa atgtaattag acctaatatt tcaggaatta tgggagcttt tggttcagca
     1801 cttatagcaa aagaaaaata tgaaagtgga tacaaaacat ccttgttatc taaggaagag
     1861 ttgaataata tcaaattaga agcaagtgta actagatgta aagggtgttc aaatcattgt
     1921 cttcttacaa taaacaaatt ttctgataat gaaattttta tttcaggtaa tagatgtgag
     1981 aaaggtgaag ctatttatgg cgataaaaaa atagggttag aaaagaaaaa accaataaat
     2041 ttatttaagt acaaatataa taggattttt agatacaaac cattggaaaa agaagaagca
     2101 aaaaatgggg aaatagggat accaagagta ttaaatatgt atgaagatta tccattttgg
     2161 ttcacttttt tcaatgctat gggctttaga gttgtacttt cagataggtc atcaaaacag
     2221 ttatatgaaa ctggtataac tagtattgca tctgaaacag tttgttatcc aggaaaatta
     2281 gttcatggac atatagaaaa tttaattgca aagggaataa aaaatatatt ttatccaagt
     2341 gtaacaaatg aaaataaaga ggattcaaat gcagataatt attataattg tcctgtagta
     2401 atatcatatt ctgaagtaat aaaaaataac gtagagaata taagaaataa gaacataaac
     2461 tatataaatc catttataag tctaaatgat aaggaaaaat tgaagaaaag actatatgat
     2521 gagttatcaa gacatttttc cgatataaaa ataactaaag aagaaattaa taatgcagtt
     2581 gataaagcta ctgatgaaca gaatttattt aaaatggaga tacaagcagc aggtgaaaga
     2641 gcacttaaag aaataaaaga aagaaatatg aagggtattg tgttatgtgg aagaccatat
     2701 catatagacc cagaaataaa tcatggaatg ccagagctta taaattcatt agatatggca
     2761 gtgcttactg aagattctat atgtcactta gccaatatac aaagaccact tagagttgta
     2821 gaccaatggg tatatcattc aagattgtat aaagcagcca gttttgtaag agataaaaac
     2881 tatttggaac ttgttcaact taattcattt ggctgtgggt tagatgcagt aactacagac
     2941 caggttcaag aaatacttaa tgaaaaatcg aagatatata ctattataaa gattgatgaa
     3001 ggcaacaatt taggagcagc taaaataagg atgaggtctt taaaagcagc tatgcttgag
     3061 agagaaagca aaaacataga tgtaaaaaat ataaaagtaa ataggataca gtatgctaaa
     3121 aataatcata taacgaacaa acatactata ttagcaccac aaatgtctcc aatacatttt
     3181 caattccttg aaaaagctgt taacttaagt ggttataata tagaaattct aaaagatact
     3241 gatagtagtg taattgaaga agggttaaga tatgtaaata atgatgcatg ttatccagct
     3301 ataatagtag taggtcagct aataacagca ttaaaatctg gcaaatatga tttaaataat
     3361 acttctgtta ctataactca gactggtgga ggttgtagag ccacaaatta tattggattc
     3421 ttaagaaagg ctatgtatga tgctggattt aaagatgttc ctgtcattgc acttagtgta
     3481 aatggaatag aagatagtgg tataatggac aatatatcac taaaattaat caatagactc
     3541 tttatgtctg ttgtttatgg tgatttgctg atgaaagtac tatatagagt aagaccatat
     3601 gaaaaagtta ctggaagtgc aaatgctcta tatgaaaaat gggttgatat atgcaaaact
     3661 tctcttgtta aagcaaaaat ttctgtattt accagaaata taaaagatat agtaaaagaa
     3721 tttgataatt tagagatact agatatcaaa aaaccaaaag taggtttggt tggagaaata
     3781 ttggtgaaat tccatccaat tgcaaataat aatttagtag atatattaga gcgagaaggt
     3841 gctgaagcag tcgtaccaga ccttacaaat ttcttcttaa gttgtgcctt taatactata
     3901 tacaaacaca ctcatttaga aggaagtaga aagagtagaa tgataggaga agtatttata
     3961 tacataactg gaatatatca aagagtatat aaaaaagcac ttgataagag tgagagattt
     4021 tatgctccag ccaatataaa aaatgttgct aaaagtacag aaccagtagt gtcattaggt
     4081 aatcaaaccg gagaaggttg gcttttaaca ggtgagatgg ttgaattatt aaatgaaggt
     4141 gtagaaaaca ttatttgtat gcaacctttt ggatgtttac ctaatcacat aattggtaag
     4201 ggttctataa aagaattaaa gaggttgtat aaaaatgcaa atataatacc tattgattat
     4261 gacccttctg caagtgaggt aaatcaactt aacaggataa aacttatgct ttctaaggct
     4321 tttaaaaata tataagattt atattttaat atgttaaaag ttaatgaatt taaaaatata
     4381 ctctatatta agtatctata aaaaagagtt atcctatttt ttagggtaac tctttattag
     4441 ttaaaaaatt atatgtatac tgatattaaa ggggggaagt aatatgagtt tagtatatga
     4501 tgtttataaa tcaaaattag gaaatttgta catattaagt gaagatgatt ttatagttag
     4561 tgtatacata ggagatgaaa aatttaataa tttaaaaaat gaatatatta aaaaaggaat
     4621 tgatataaag agaagtaagg aagatttaaa ggaagcaata agtcagatag atgattactt
     4681 taatggaaaa agaaaagtct ttaatttaaa tattcgtatt gaagggactg aatttcaaaa
     4741 atcagtttat agggaaatgt taaaaatacc ttatggagaa acattgtgtt attcagatat
     4801 agcaagaaat ataaataatc caaaatcagt aagagcaata ggacaagcaa gtaaaagtaa
     4861 taaaattcca ataataatac cttgtcatag agtagttggc aaaaaaaata ttggtggata
     4921 tatggggaat cattctgatt tgaaagagat actattaaat ttagaaagac agagttaact
     4981 tagaaagaaa tgtttttatt gttaatattt gatataataa tattaaaaat aagtattttt
     5041 tacaaaaaca ataaagaatt tattttaatt tacaaaaatg agggggaatt ttaatgtgtg
     5101 ataaagttat agcatcaaaa tatcatgaga agggatttaa ttgtgcagaa tcagttataa
     5161 aagcatataa tgaagaattt aatacagata taccagtttg cctaggtagt ggactgggtt
     5221 cgggttgtgg agttgctagt ttatgtggtg cagtaaatgc atcaaatata ataattggat
     5281 acgtaaaagg cagaaaccat gaagatgaaa gtactagagc taaagtatat gctaaagatt
     5341 taactacaac agtaagaaaa gagtatggtt cagagttatg tatagaccta aaaaaagatt
     5401 tagttgcctg tagagaaata atggatttcg catatgatag tctaaaggaa actttaaaaa
     5461 aagaattata agaaaataag ctatcaagta caaattattt gatagcttat ttttatagtc
     5521 tatactaaat aaattaaaca tattttagaa ttattatttg taaataaaca tcaaataatt
     5581 ttaggttttt tatttaatat agctttttat ttatatttgt ttttgaatgt gttatactct
     5641 gtttaaataa atatttgtaa taagggagat gagaagattg gataatatca atttgcttat
     5701 taatagatta tattcaaaaa atcataatga agcatataaa acttttttat tcttagaaaa
     5761 tgaaagctta aaatcaaata ttacatattg tttttttgac tcattcctag aaatgattaa
     5821 taacgaaaac tcatacattc gagcaagagg attgttatta atttccgcaa atgctcaatg
     5881 ggatattgat aataaaattg aaataaatat agatagcata ctaagtcata tcgtagataa
     5941 aaaacctttt gtatcaagaa tgtttattaa atccatacct aatattacaa agtataaaaa
     6001 aaatctcatt cgtcgcataa aaatggaatt aagtaatgca gatataagta tttataataa
     6061 taatatgaaa ccattagtag aaaaggatat aaacgataca ttatctaatc ttagttaaat
     6121 attaatatag aaattggata ttatctagtt gcaattattt ataagtaaaa taataaaaca
     6181 ataatggtgc taatttttat agataaatta attatgaaaa atttatgtaa gtaaaatata
     6241 ttaacataaa ggtaaaattt tgtacactaa aagggaatat ttacagttta atggcagttt
     6301 tgaaataaca agttaaagct gtcattttta tttgaaaatt tttagaaaaa gtacaatcgt
     6361 gttaatctgt taactagata aggtttatat aataatttgg atga
//
LOCUS       DAFNNB010000173         6347 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0178, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000173 DAFNNB010000000
VERSION     DAFNNB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6347
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0178"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(129..1574)
                     /locus_tag="L3M60_003148"
     CDS             complement(129..1574)
                     /locus_tag="L3M60_003148"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBN6227780.1"
                     /translation="MIDNKYTTYSNPLTDRYCSKDMSYIFSPQFKFSTWRRLWVALAE
                     GEHELGINITEEQLDELRANIDNINFDEARKIEKEVRHDVMSHVKAYGLQCEKAKGII
                     HLGATSAYVGDNTDVIQMNEALKLLRVKLVNLINNLKEFALKYKDMPALGFTHFQAAQ
                     LTTVGKRACLWAQDLILDLEDLNYRIDNMKLRGVKGTTGTQASFLSLFEGDHEKVKEL
                     DKKICKKMGYNSSYAVSGQTYTRKLDYQVVSILSGIAQSMHKMTNDIRLLQSLKELEE
                     PFEKNQIGSSAMAYKRNPMRSERIASLSKYIISESISPAMVQATQWLERTLDDSANKR
                     LAIPQAFMAADAILEIGINVTDGLVVYENMINKRVNEELPFMATETILMEAVKRGGDR
                     QELHEIIREYSMKAAYRVKHEGKDNNLIELIMNDDSFKMSKEEILSIMDPKNFIGRAP
                     EQVVEFVNEVVEPAIKDYKEDLGKVDVDLRV"
     gene            complement(1713..2624)
                     /locus_tag="L3M60_003149"
     CDS             complement(1713..2624)
                     /locus_tag="L3M60_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6227781.1"
                     /translation="MYSTKILDDYEYETFNVFSTKLNPNTKHDYLSKVILFKEFLKGK
                     ELIYATKEDCKNFVDYIQTKYAKSTCEKIYSYLHSFYNFLKKEGYIDINPFRYVEKPT
                     VTRIKTKDDVLSIQEINKLIGILPKLNIRDRVIIVCLVTTGCLLNELVSLKWKDLMVD
                     ENDNSYVRLGKGRKERVVKLHPYFFKLLEDYRNYSGLPEVIIPSDDFIFTTQKSNSIT
                     DRNVRLIVKKALDLAGLSQYSARDFRHSFAAISLRLGADESDVKNQLGWSDKYYAIRY
                     KYVLNFVDSEIVDYLIEKDNLDINKKY"
     gene            2881..3705
                     /locus_tag="L3M60_003150"
     CDS             2881..3705
                     /locus_tag="L3M60_003150"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBN6227782.1"
                     /translation="METFSLLEIFKAVILGIVQGITEWLPVSSTGHMILVDEFIKLNF
                     SNTFISTFLVVIQFGSILAVLVIFFRKLNPFDSAKNIKQKKETVRLWLKVIIAVIPSG
                     VIGILFEDDIDRLFFNSTVVAIALIVYGIIMIGLEKRNKRPKYKDFSQVTYKLALCIG
                     LFQCLALIPGTSRSGSTIIGAVLLGTSRYVAAEFSFFLAIPTMLGASALKLLKAGFGF
                     TGFEWLILGVGSVVAFVVSIVVIKFFMDYIKKHDFKVFGYYRIVLGIVVLAYFFLL"
     gene            3745..4275
                     /gene="lepB"
                     /locus_tag="L3M60_003151"
     CDS             3745..4275
                     /gene="lepB"
                     /locus_tag="L3M60_003151"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBN6227783.1"
                     /translation="MSVKKEIFDWIKSIAMAIVLAFVILQFIRPSIVSGESMYPTLDD
                     KDYLILNRISYKVGKPEKGDIVVFKTNLVDGETGKKKDLIKRIIATEGDRIKISNSKV
                     YVNGKLLNEPYIHNNYTSGDIDTVVPKGKIFAMGDNRENSNDSRFPDVGMIDEDEILG
                     KVMVRLLPLDNIGKVD"
     gene            complement(4355..6136)
                     /locus_tag="L3M60_003152"
     CDS             complement(4355..6136)
                     /locus_tag="L3M60_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HBN6227784.1"
                     /translation="MSQYININSQLDALKLKEFEKIFYENINKDVKVVPKVTPFKGIN
                     TDLLYVKDGKILFIKFMDTTEDIFFILEEELLEVMNEEYELLKLKMGQKNRNISYNYV
                     YIMPYVEVEETYEFEEFVNNNIIDKNKLQDIMNKGSLDEYLNDENDEINLNLFLLDVC
                     SEYYIINDKLHLNEKFKKISFYNDDYKYTATMMEEVQIKDVISIKYGNTLIEGGSGIG
                     KTAIMLSRAIKLAKVYPHHKLIIFTHTKQLRNELRERIELLYKDNNNLEVHTFSSFIF
                     KLAKKFNLIVDYNMLKNDYEKAFNNLVKQAQNIIKNKNMFKAIFIDEAESFLEYEIDF
                     IREFLYKTKFIFNVCSCNSLNISNRLNIFKKLYNGIEFDDKIILSKNYRQAKEIVDFT
                     NKFSNNSNSYINELRPNTEFSTFFYTKALRGGNKSVDIIKVSDLDDQISSVIWEIEYL
                     ISKKGLDYSEVAIVYPYNKKKLKSGKTIYFQYMLKKALEEAKIPYICAEDNLTNISKK
                     VGTTIANIYAIKNLEYKAVIVCELEMLYNQTINDIEQDYQVNDFVGDLNKVYLAMSRA
                     TDYLSIVTTFNEEASDIIRLITESKDI"
BASE COUNT         2061 a          949 c          760 g         2577 t
ORIGIN      
        1 taaattcatg gtatagtaag taaagaagaa ctcttcacag cggtgaagag aagacgctag
       61 cccattaaaa cggctatctt atttataaat aagatagccg tttttaatta tattttatct
      121 gtataattct atacccttaa gtctacatct acttttccta agtcttcttt ataatctttt
      181 atagcaggtt ctacaacttc atttacaaac tctacaactt gttctggagc tctacctata
      241 aagttttttg ggtccattat agataatatt tcttctttgc tcatcttaaa tgaatcatca
      301 ttcattataa gttcaatcaa attattatcc ttaccttcat gttttactct ataggcagct
      361 ttcatagaat attctcttat tatttcatgt aattcctgtc tatctccacc tctttttaca
      421 gcttccataa gtattgtttc tgtagccata aaaggaagtt cttcatttac acgtttattt
      481 atcatatttt catatactac taaaccatca gttacattta tccctatttc aagtatagca
      541 tctgctgcca taaatgcttg aggtattgcc aatctcttat tagctgagtc atctaatgtt
      601 ctctctaacc attgagtagc ttgtaccatt gcagggctta tagactcaga gattatatac
      661 ttagataaag atgctattct ctcacttctc attgggtttc tcttataagc cattgctgat
      721 gaacctattt gatttttttc aaatggctct tctaattctt ttaaactttg taaaagtctt
      781 atatcattag tcattttatg catactttga gctatgcctg ataatatgct tactacttga
      841 taatctaact ttctagtata tgtctgtcca cttactgcat aagaagaatt gtatcccatc
      901 tttttgcaaa ttttcttatc taactctttt actttttcat ggtctccttc aaataagctt
      961 aaaaaacttg cttgagtacc agtagtacct ttaactcctc ttaacttcat attatctatt
     1021 ctatagttta aatcttccaa atcaagaatt aaatcttgag cccataaaca agctcttttt
     1081 cctactgttg taagttgagc agcttggaag tgtgtaaatc caagtgctgg catatctttg
     1141 tacttaagtg caaattcttt taaattgttt attaaattta caagctttac tcttagaagt
     1201 tttaaagctt cattcatttg tataacatct gtattgtctc caacatatgc agacgttgcc
     1261 cctaaatgta ttattccttt ggctttttca cattgtaacc cataagcttt tacatgagac
     1321 attacatcat gtcttacttc tttttctatt tttctagctt catcaaaatt tatattatct
     1381 atattagctc ttagctcatc taattgttct tcagttatat tgattcctag ctcatgttca
     1441 ccttctgcaa gtgcaaccca taacctcctc catgtagaga atttaaattg tggagaaaat
     1501 atataactca tgtccttgct acaatatcta tctgtaagtg ggtttgaata agtagtatat
     1561 ttattatcta tcattatatt agcaccttcc ttaaatcttt tgtcgtatga ttactgtgta
     1621 cacattagta cataaataat tatatataat tttacgaact ataacaagac ttttttattt
     1681 tttgttcata tttatatagt gttttttatt tattaatatt ttttatttat atccaaatta
     1741 tccttttcaa ttaagtaatc cactatttca ctatcaacaa aatttaatac atacttatat
     1801 ctaattgcat agtatttatc actccatcca agctggtttt ttacatcgct ctcatctgcc
     1861 ccaagcctta aacttattgc tgcaaatgaa tgtctaaaat ctcttgctga atattgagaa
     1921 agcccagcta aatctaatgc ttttttgact ataagtctga catttctatc tgttatagaa
     1981 ttactctttt gagttgtaaa aataaaatca tcactaggta ttattacctc tggaagccca
     2041 gaataattcc tgtaatcttc taaaagttta aaaaagtatg gatgtaattt aactactctt
     2101 tcttttcttc ccttacctaa tcttacatat gagttatcgt tttcatctac cataaggtct
     2161 ttccacttta agctaactaa ttcatttaat aaacacccag tagtaactag acagactatt
     2221 attactctat ctcttatatt taattttggt aggataccta taagtttatt tatttcttgt
     2281 atacttagaa cgtcatcttt tgtttttatt cttgttactg ttggtttttc tacatatcta
     2341 aatggattta tatctatgta tccttctttt tttaagaagt tataaaaact atgtaaataa
     2401 ctatatatct tttcacaagt tgatttcgca tatttagtct gtatataatc tacaaaattt
     2461 ttacaatctt cttttgtagc ataaatcaat tccttccctt ttaaaaactc tttaaacagt
     2521 ataacctttg ataaataatc gtgttttgtg tttggattta attttgtaga aaaaacatta
     2581 aaagtttcat actcataatc atctagtatt tttgtactat acataataac tcctttcctt
     2641 ttcataaaat aaaataacta tacttttact ataccatata tttccccctc aaatataccc
     2701 cttattcaag ggatgacttt taagtttatt ttgttatatt atgaaggttt tatgaaaatg
     2761 tcaatcttcc attgtgcact acacagtact atggtattat aagttagtaa aaggtggcat
     2821 ttttttatta atatatgaat aaaatatgct actataaatt tgtattggag gtattttggt
     2881 ttggaaacat ttagtttgct tgaaattttt aaggcagtca ttttaggaat agttcaagga
     2941 ataacagaat ggctaccagt aagtagtaca gggcatatga tattagttga tgagtttata
     3001 aaattgaatt tttcaaatac atttataagt acatttttag ttgtaattca atttggttca
     3061 atattagctg tacttgttat attttttaga aaattaaacc cttttgatag tgcaaaaaat
     3121 attaagcaga aaaaagaaac agttcgttta tggcttaagg ttataattgc agttatacca
     3181 tctggagtta ttggaatact gtttgaagat gatatagata gattattctt taattcaaca
     3241 gtagttgcta ttgctcttat tgtctatggt ataataatga tagggcttga aaagagaaat
     3301 aaaagaccta agtataaaga tttttctcaa gttacatata aattagctct ttgtatagga
     3361 ttattccaat gtctagctct tataccaggt acatctaggt ctggttctac tataataggt
     3421 gcagtattac ttggtacttc tagatatgta gcagcagagt tttcattctt cttagctatc
     3481 cctacaatgc ttggagctag tgcattaaaa cttctaaaag ctggatttgg atttactgga
     3541 tttgaatggc ttattttagg tgttggttct gtggtagcat ttgttgtttc aattgtagtt
     3601 attaaatttt ttatggacta tatcaaaaaa catgacttca aagtatttgg ttattataga
     3661 atagtcctgg gtatagtggt tcttgcttat ttctttctat tataatgtat tctaatttaa
     3721 taacaaatga taaggagaaa agttatgagt gttaaaaaag aaatatttga ttggattaag
     3781 tcaatagcta tggctattgt acttgcattt gtaattctac aatttataag accttctatt
     3841 gtcagtggag aatcaatgta tcctacttta gatgataaag attacctaat tttaaatagg
     3901 atatcataca aagttggtaa acctgaaaaa ggcgatattg tagtttttaa aaccaattta
     3961 gttgatggag aaacaggaaa gaaaaaagac ttaataaaaa gaattatagc tactgaaggt
     4021 gacagaataa aaatatcaaa ttctaaggtg tatgtaaatg gaaaattatt aaatgagcca
     4081 tatatacata ataactatac ttctggagat atagatactg ttgttccaaa aggtaaaata
     4141 tttgcaatgg gagataatag agaaaatagt aatgatagta gatttcctga tgtaggtatg
     4201 attgatgagg atgaaattct tggtaaggtt atggtgagac tattacctct tgataatatt
     4261 ggaaaagtag attaaaaaaa agagactgaa ttggtaaaat ttattttaca aatctcagtc
     4321 tctttttatt tacttaaatt aaaaatattt tttactaaat atcttttgat tctgtaataa
     4381 gtcttattat atcacttgct tcttcattaa aagttgttac tatacttaag tagtctgttg
     4441 ctctactcat agctaaataa accttattta aatctcctac aaaatcattt acttgataat
     4501 cttgctcaat atcatttata gtttgattgt ataacatttc taattcacat actattactg
     4561 ctttatattc taaattcttt attgcatata tatttgctat tgtagttcct accttcttac
     4621 ttatatttgt taagttatct tctgcacata tataaggaat ttttgcctct tctaatgctt
     4681 tttttaacat atactggaaa taaatagttt ttccactttt taatttcttc ttattataag
     4741 gataaacaat agcaacttcg gaataatcaa gacctttttt acttattaaa tactcaattt
     4801 cccatattac agaacttatt tggtcatcaa ggtcacttac ttttattata tcaacagact
     4861 tatttccacc tctaagtgct ttagtataaa agaaagtact aaattctgta tttggtctta
     4921 attcgttgat atatgagtta gaattatttg aaaatttatt tgtaaaatct acaatctctt
     4981 tggcttgtct ataattttta cttaagataa tcttatcatc aaattctata ccattatata
     5041 actttttaaa tatatttaat ctattagaga tattcaaaga gttacaagaa cacacattaa
     5101 atataaattt tgttttatat aaaaactctc ttataaaatc tatttcatac tctaaaaagc
     5161 tttctgcttc atctataaaa atagctttaa acatattttt attttttata atgttctgtg
     5221 cttgttttac aagattatta aacgcctttt cgtaatcatt ttttaacatg ttatagtcta
     5281 caattaaatt gaatttttta gctagtttaa atataaaaga actaaatgtg tgaacttcta
     5341 aattattatt atctttatat aacaactcta tcctctctct aagctcattg cgcaattgtt
     5401 ttgtatgtgt aaatatgatt aatttatgat gtggatatac ttttgctaat tttatagctc
     5461 tagaaagcat aattgctgtt ttacctatac cagagccacc ttcaataagt gtattcccat
     5521 atttaataga aataacatct ttaatttgaa cttcttccat catagttgct gtatacttat
     5581 agtcatcatt gtagaatgaa atttttttaa atttttcatt taaatgaagt ttgtcattta
     5641 ttatatagta ttctgaacat acatctaata aaaataaatt taagtttatc tcatcattct
     5701 catcattaag atactcatcc agcgaacctt tattcattat atcttgaagc ttattcttat
     5761 ctattatatt gttattaaca aattcttcaa attcatatgt ttcctcaact tcaacgtatg
     5821 gcataatgta aacgtagtta taacttatat ttctattctt ttgtcccatt tttagtttca
     5881 aaagttcata ttcctcattc ataacttcaa gtaactcttc ttctaaaata aaaaatatat
     5941 cttccgttgt atccataaat tttataaata aaatttttcc atctttaaca tacaacaaat
     6001 cggtatttat tcctttaaaa ggagtaactt ttggcactac tttaacgtcc ttattgatat
     6061 tctcgtaaaa tattttctca aattctttca attttagggc gtctaactga gaatttatat
     6121 ttatgtattg gctcacttac attacctcct tctgttatac ttaattatac cattttgaac
     6181 tatattttta tatatacttt ttataggcaa atttagtaaa taaattcaaa ataaatacaa
     6241 ataaatacaa aacagctaaa aaaattttgc aataagtaca gatatttatt gttatagaat
     6301 attttatgta aatagtaaaa tgatgaattt cataattaat acttaca
//
LOCUS       DAFNNB010000174         6279 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0179, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000174 DAFNNB010000000
VERSION     DAFNNB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6279
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0179"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            212..865
                     /locus_tag="L3M60_003153"
     CDS             212..865
                     /locus_tag="L3M60_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HBN6227785.1"
                     /translation="MKAEKTSIYPIIKNNLNKISIVEYKKGQRFVASDKDLQEVFFII
                     EGVALVECTTRGGNKFLVDIVPENEFIGKISYIYEHNLKCDIFAKTNIKLFRFEKNVF
                     EEFYLKPDFIALFHRKCTRRIYELYKTRMVRELFSCTEVIAYCILLNKQENNVCNIKA
                     IRNFSEIYSISRKSRYHSLLRLTEKGIIKKMGNTYEILNYDELHRLAFEVKEFLEDE"
     gene            complement(1005..2381)
                     /locus_tag="L3M60_003154"
     CDS             complement(1005..2381)
                     /locus_tag="L3M60_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003425390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6227786.1"
                     /translation="MQELFSLKDENKRFYKILLSLCIPIIIQNLISTSVNVIDTIMIS
                     SLGETSVASVGVANQFFFLFNMSLSGITGGAGVFISQFYGKKDVGNIRKVTGLTCMLA
                     IILSFVFVIPALLTPKPIIHIFSYDSEVVKLCIDYFSIAVFSYPLIAISTVFSTGSRG
                     VRNPKLGMICSAFALVTNVILNYGFIFGNFGLPALGVKGAALATVIARICELILMITY
                     VYFYKKDYILKFGLKNLKAIDKIFIKSFSSKSFPIFVNDSVWAVGTVLYSVAYARAGT
                     SAIAASQIATSTGNFFIMTAVCIASGASIMLGNELGADHIKRAIEYAKKFSILVFSAG
                     LILGIILILNIPLLLKMFSVSDSLASDITKIFFIMGILMALKSFNTLVIIGILRSGGD
                     TKYALFLELGCMWLASIPLTFIAAFKGAPIFVLVLLTYSEEVVKFIFGVPRALSKKWA
                     INIVKEID"
     gene            complement(2397..4310)
                     /locus_tag="L3M60_003155"
     CDS             complement(2397..4310)
                     /locus_tag="L3M60_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBN6227787.1"
                     /translation="MIVLSCNNLNKSFGIDSILENISFTVNEGDKIGIIGVNGTGKTT
                     LFKIISGIYGYDSGDIYTSKDCEIGYLEQNTNFYSDNTILEEVLEVFKNLIDMESYLR
                     ELEVKISEESTKTNSPIIEKIMDEYSHKLELFSDLNGYGYKSEAKGVLKGLGFSDNDM
                     DKPISILSGGEKTRVLLGKLLLKKPTLLLLDEPTNHLDSEAIEWLEVFLKQYKGTVML
                     ISHDRYFLDQSVNRIFEVHNKKLKVYNGNYSKFVELSKIEKELELKKFEDQQKEIKKQ
                     EESIERLKAYGREKHLKRARSKEKALDKVDVLDKPEAYRKKARIQFTPSVQSGNDVLQ
                     IRDVSMGYGERILFKDLDLDIYRGEKVALIGANGVGKSTLFKIITNELKPLSGNIKFG
                     TNVHVSYFHQEQKTLNLDNTIIDEIWENNTHLTQTTLRNMLGAFLFVDEEVFKKISTL
                     SGGERARVAILKLILSNANLLLLDEPTNHLDIDSKEVLEEALTNYDGTIFTISHDRYF
                     LNTVVDKILVLDENGITEYLGNYDYYIDKKRQIQEMSIIEEKEEKTRTQIKDEKRKER
                     EQREIEKKNRIKRQNIEKEIEKLELEIEKLDILLCQEEVYSNPDKAKEVSQEKINLEN
                     NLASLYDEWEEFM"
     gene            4446..5078
                     /locus_tag="L3M60_003156"
     CDS             4446..5078
                     /locus_tag="L3M60_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HBN6227788.1"
                     /translation="MLGNKNISMAVIRRLPKYHRYLGDLLDRDIQRISSKELSDIIGF
                     TASQIRQDLNNFGGFGQQGYGYNVEALHTEIGKILGLDRPYNAVLVGAGNLGQAIANY
                     AGFRKAGFEIKALFDANPRMIGLKIREFEVLDSDTLEDFIKNNNIDIAVLCIPKNGAQ
                     EVINRVVKAGIKGVWNFAPLDLEVPKGVIVENVNLTESLFTLSYLMKEGK"
     gene            5217..5756
                     /locus_tag="L3M60_003157"
     CDS             5217..5756
                     /locus_tag="L3M60_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HBN6227789.1"
                     /translation="MIFVEELWEKAIEFHGHECPGLAIGFKAALAAREYLGCKQSGDE
                     EIVCISENDACGVDGIQVVLSCTMGKGNLLLRMTGKSVYHFFNRENGKSIRIYVKSKP
                     FEMDREGYKKYLLESDFEEIFEITDTKLRLPEKARIFKSLKCEVCGEFASEQYIRIEE
                     GKKVCLDCFNKYERFYEEY"
     gene            complement(5922..6092)
                     /locus_tag="L3M60_003158"
     CDS             complement(5922..6092)
                     /locus_tag="L3M60_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HBN6227790.1"
                     /translation="MAYKITDACISCGACEAECPVSCISAGDDAYVIDASSCIDCGSC
                     AGACPVDAPQPE"
BASE COUNT         2329 a          838 c          876 g         2236 t
ORIGIN      
        1 taaattctat tgataatata attttataaa tatagaattg tattaaaata aattatatat
       61 gaaaatatat atgtataagt ttattctgcg tgagtagaat gaatttgtca agtttaattt
      121 aactataatc atatctttta ttaaaaataa tataaattaa aacatataaa taaaatattt
      181 tatacaatat attgtaaagg aggtaacttt gatgaaagca gaaaaaacaa gtatatatcc
      241 aattattaaa aataatttaa acaaaatatc aattgttgaa tataaaaagg gtcaaagatt
      301 tgtagcatca gataaagact tgcaagaagt attctttata atagagggag ttgcattagt
      361 ggagtgcact actagaggag ggaataaatt tcttgttgac attgtacctg aaaatgaatt
      421 tataggcaaa atcagttata tatatgaaca taatttgaaa tgtgatatat ttgcaaaaac
      481 caatataaaa ttatttcgat ttgaaaaaaa tgtatttgaa gagttttatc ttaaaccaga
      541 ttttattgca ctatttcaca gaaaatgtac aagaagaata tatgaattat ataaaactag
      601 aatggtaaga gaactatttt cttgtacaga ggttattgcc tattgtatat tattaaataa
      661 acaagaaaat aatgtctgca atataaaagc cattcgtaat ttcagtgaaa tctattcaat
      721 tagtagaaaa agtagatacc attctctgtt gaggttaact gaaaaaggta taataaaaaa
      781 aatgggtaat acatatgaaa ttttaaatta tgatgaatta catagattgg catttgaagt
      841 aaaagagttt ttggaggatg aataaagatt attacttaat caaaataagg tataataatt
      901 tctgtaaaaa atagaaatag tctcactgaa aatcaaaaat actttagttg ttttcaatga
      961 gactgtttag aaatattgta tacttacaat gtatagtata gttattagtc aatttccttt
     1021 actatgttaa tagcccattt tttagataat gctctaggaa caccaaatat aaacttaaca
     1081 acttcttcac tgtaagtgag tagaacaagt acaaatatag gtgcaccttt aaatgcagct
     1141 ataaaagtta gaggaataga agctagccac atacatccta attctaaaaa tagagcatat
     1201 ttagtatctc ctccacttct taaaatacca attataacca atgtattaaa agatttgaga
     1261 gccattagta ttcccattat aaagaatatc ttagttatat cagaagctaa gctatcagaa
     1321 acactaaaca tttttaataa taatggtata tttaaaatta aaataattcc aagtattaaa
     1381 ccagctgaaa atacaagtat agaaaacttt ttagcatatt caatggctct tttaatatgg
     1441 tcagctccaa gttcatttcc aagcataata gatgcaccag atgcgataca tacagcagtc
     1501 attatgaaga aattgcctgt actggttgct atctggctgg ctgctatagc agaagtacca
     1561 gctcttgcat atgcaacaga atacagcaca gttccaactg cccatacaga gtcatttaca
     1621 aatataggaa agctttttga tgaaaaagat tttataaaaa ttttatcaat agcttttaga
     1681 tttttcagac caaattttaa tatatagtct tttttataaa agtaaacata agtaatcatt
     1741 aaaattaatt cacatattct agcaataaca gtagctagag cagcaccttt tacacccagt
     1801 gcaggaagac cgaagttacc aaagataaat ccataattta aaataacatt tgttacaagt
     1861 gcaaatgcac tacaaatcat acctaactta ggatttctaa cacctcttga accagtacta
     1921 aatacagtac ttatagcaat taatggataa ctaaatactg caatactaaa gtaatctata
     1981 caaagtttta caacttcaga atcatacgaa aatatgtgta taattggctt tggtgttagt
     2041 aaagcaggaa taacaaatac aaaacttaaa ataatggcta acatacaagt aagacctgta
     2101 acttttctta tattgccaac atccttcttt ccataaaact gtgaaataaa aactccagca
     2161 cctcctgtga tgccagataa ggacatatta aaaagaaaga aaaattggtt tgcaactcca
     2221 actgaagcta cagatgtttc tcctaaacta cttatcataa ttgtatctat tacatttaca
     2281 gaagtagaaa ttaaattctg tataataata ggtatacata aagatagaag tattttataa
     2341 aatcttttat tttcgtcttt taaagaaaat agttcttgca aataatcctc tcctttttac
     2401 ataaattctt cccactcatc atatagagag gcaaggttat tttctaaatt aattttttct
     2461 tgactaactt ctttagcctt atctggatta gagtatactt cttcttgaca taaaagtata
     2521 tctaattttt caatttcaag ctctaacttt tcaatttctt tttctatatt ttgtcttttt
     2581 attctatttt tcttttctat ttctctttgt tctctctctt ttcttttttc atcttttatt
     2641 tgagttctag ttttttcttc tttttcttct ataatactca tttcttggat ttgccttttt
     2701 ttatctatat aataatcata atttcctaga tattcagtaa ttccattttc atctaaaact
     2761 aaaattttat caacaacagt atttagaaag tatctatcat gtgatattgt aaatatagtt
     2821 ccatcataat tagttaatgc ctcttcaagg acttccttag aatcaatatc taggtgatta
     2881 gtaggctcat ctaataataa taagtttgca tttgatagta taagtttaag tattgcaact
     2941 ctagctcttt ctccaccgct caatgtagaa attttcttaa aaacttcttc atctacaaat
     3001 aaaaatgctc ctaacatatt tctcaaagta gtttgagtaa gatgagtatt attttcccaa
     3061 atttcatcaa ttattgtatt atctaaatta agagtttttt gttcctgatg gaagtaggaa
     3121 acatggacat tagttccaaa tttaatattg ccactaagag gtttcagttc attagttatt
     3181 attttaaata atgtagattt accaacacca tttgcaccta taagagcaac tttttcccct
     3241 ctatatatat ctaaatccaa gtctttaaaa agtattcttt ctccataacc catagataca
     3301 tctctaattt gaagaacatc attaccactc tggacagaag gcgtaaattg tatacgagct
     3361 ttttttctat aagcttctgg tttatctaaa acgtctacct tgtctaatgc tttttcttta
     3421 cttcttgcac gctttaggtg tttttctcta ccataagctt ttagtctttc aattgattct
     3481 tcttgctttt ttatttcttt ttgttggtct tcaaatttct taagttctag ttctttttct
     3541 attttagata actcaacaaa tttagagtag ttaccattat atactttaag ttttttgtta
     3601 tgaacttcaa aaattctatt tacagattgg tctaagaaat atctatcatg agatattaac
     3661 atgacagtcc ctttatattg ttttaaaaat acttctaacc attctattgc ttcagagtct
     3721 aagtggttag taggttcatc aagtaacaat agagttggtt tcttaagaag taattttcct
     3781 aaaagaactc tagttttttc accaccagaa agtatactta ttggcttatc catatcattg
     3841 tcactaaatc ctaatccttt tagaactcct ttggcttcag acttatatcc atatccattt
     3901 aaatctgaaa ataattccag tttatgtgaa tattcatcca taattttttc tataattgga
     3961 gaatttgttt ttgtactttc ttcagatatt ttaacctcca attctcttaa ataggactcc
     4021 atatctatca aatttttaaa aacttctagt acttcttcta aaatagtatt atccgaataa
     4081 aaattggtat tttgttctaa atatccgatt tcacaatctt tagaagtgta aatatctcca
     4141 ctatcatatc cataaattcc tgaaattatt ttaaataaag tggtttttcc agtaccatta
     4201 acaccgatta taccaatttt atcgccttca tttactgtaa aactaatatt ttctaatata
     4261 gaatctatac caaaactttt atttaagtta ttacatgaca aaacaatcat aaatttctac
     4321 ctccttgaac tatctaaaat tataatgttt tgatataata taatcaaggt tatatgtatt
     4381 agttttgaaa aagaaatatt ttattgtgaa tcaaacaaaa aagaggacac ttttaggagg
     4441 tgaagatgtt gggaaataaa aatatatcaa tggcagttat aagaaggcta ccaaaatatc
     4501 atagatatct tggagactta ttagataggg atatacaaag aatatcttct aaagaattga
     4561 gtgatataat agggtttacc gcttctcaaa taagacaaga tttaaacaac tttggtggat
     4621 ttggacaaca agggtatggt tataatgtag aagctcttca tactgagata ggtaaaattc
     4681 ttgggttgga tcgaccatat aacgcagttc ttgtaggagc aggtaactta ggacaagcta
     4741 tagccaatta tgcaggattt agaaaagctg gattcgagat aaaagcttta tttgatgcaa
     4801 atcctagaat gataggttta aagataagag agtttgaagt attagattca gatactttag
     4861 aagactttat aaaaaacaat aatatagata ttgctgtatt atgtatacct aaaaatggag
     4921 cacaagaagt tattaataga gttgtaaaag ctggaatcaa aggtgtatgg aattttgcac
     4981 ctttagattt agaagttccg aaaggtgtta tagttgaaaa tgtaaactta acagaaagtt
     5041 tatttacttt atcgtattta atgaaagaag gaaagtagac aacttaaaca tattaataat
     5101 gagtagttgt gaagagtatt gagaaagtca gaagatttaa tattaaatca tctggctttt
     5161 tgaaatatta ggataaaaat ttaaataata tatgtaaata aatagtatta aggagagtga
     5221 tttttgtgga ggaattatgg gaaaaagcaa ttgaatttca tggtcatgaa tgtccaggtt
     5281 tagctatagg atttaaagca gcacttgcag caagagaata tttaggatgt aaacaatcag
     5341 gagatgagga aattgtgtgt atttctgaaa atgatgcttg tggagtagat ggcatacaag
     5401 ttgtattaag ttgcacaatg ggtaaaggaa atttactact taggatgact ggaaagtctg
     5461 tatatcattt ctttaataga gaaaatggga aatctataag aatatatgta aagtcaaaac
     5521 catttgagat ggatagagaa ggatataaga aatatttatt agaaagtgat tttgaagaaa
     5581 tatttgagat tacagatact aagttaagac ttcctgaaaa agcaagaata tttaaatctc
     5641 ttaaatgtga ggtgtgtggt gaatttgcat ctgaacaata tataaggatt gaagaaggaa
     5701 agaaagtatg tcttgattgc tttaataaat atgaaagatt ttatgaggag tattaaaatt
     5761 ataaattaaa agtattagat atgcgctaac aatattttaa gatgaattag gtagtaacta
     5821 taataaaata tataaataat attacttgaa tcatacataa ttaaaaagag gctgattagt
     5881 atttagaaaa taaaatcagt ctctttatga ataacgaatt attattctgg ttgaggtgca
     5941 tctacaggac aagctcctgc acaagaacca cagtctatac aagaactagc atctataaca
     6001 tatgcgtcgt ctccagctga tatacagcta acagggcatt cagcctcaca agcaccacag
     6061 cttatacaag cgtcagtaat tttatatgcc attttgaaat cctccctttt atatgtattg
     6121 taattcaata acattataac aaatattgtt gaatataaca attaaattaa ttctacaaca
     6181 aaaactttat tatgatattc ttgttttaga gttttacaat aaagtttaat ctgcttatat
     6241 ttttatttat tttaaagaga tttattttaa aaataaaat
//
LOCUS       DAFNNB010000175         6262 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0180, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000175 DAFNNB010000000
VERSION     DAFNNB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6262
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0180"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            105..395
                     /gene="cas2"
                     /locus_tag="L3M60_003159"
     CDS             105..395
                     /gene="cas2"
                     /locus_tag="L3M60_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HBN6227791.1"
                     /translation="MKKKNNLNYNYVFVVYDVGEKRVSKVFKVCKKYLSHFQKSVFRG
                     EITPSKIMSLKRDLENIIDKDNDFVCIIKLMNSNTFEEEILGNNSNCENMIL"
     gene            1085..1288
                     /locus_tag="L3M60_003160"
     CDS             1085..1288
                     /locus_tag="L3M60_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227792.1"
                     /translation="MISYAPLWKLLIDRKIKKMEFVNISGISISVLGRLGNDKSVSMD
                     TMEKICLALDCKIEDVVEIKKGI"
     gene            1452..2606
                     /locus_tag="L3M60_003161"
     CDS             1452..2606
                     /locus_tag="L3M60_003161"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBN6227793.1"
                     /translation="MKKISILGSTGSIGKQTLDVVREHRDKFEIVAISANSNIELLLE
                     QIVEFKPKYVTVFEENKALKLKEMLPKNIEIEVLAGMEGLKIISSLDEVDVLLTAVVG
                     MIGLVPTLCAIKKGIDIALANKETLVTAGELVMKEAEKYNVNILPVDSEHSAIFQCLN
                     GENKKNIEKIILTASGGPFRGKKKGELVNITKNEALKHPNWSMGRKISIDSSTLMNKG
                     LEVIEARWLFGVEQENIDVVVHPQSIIHSMVQYADSSIIAQLGCPDMRLPIQYALTYP
                     DRMESSFERMNFSKFSTLTFEEPDLETFPCLKLAYECLKMGGTYSSVLNSANEVLVSE
                     FLEDKIGFYDIPYYIEKTLEVHSSISEPTLEEILETDRWSRAYVANLIKK"
     gene            2619..3623
                     /gene="rseP"
                     /locus_tag="L3M60_003162"
     CDS             2619..3623
                     /gene="rseP"
                     /locus_tag="L3M60_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HBN6227794.1"
                     /translation="MTIIAALILFSIIVLIHELGHFIFAKRSGIKVNEFSIGMGPKIY
                     SVKKDTEYSIRALPIGGYVSMEGEDEEQISPNSFGNKSILQRFSTIVAGPIFNIILAA
                     ILLVPVFLYIGSPTTKLGKIMPDTPAQAVGLQVGDKINKINGNSVKTWDEVANIINTS
                     SGGELKLSITRDGSDKVVNVTPKNNNGKYEIGIQPQREKDFWGSIVNACKTTVDMTKQ
                     MLTFLGQMITGRVPGGIGNAVAGPVGVIGMVSDAARTGIINVVYLAAVISLNLGIVNL
                     LPIPALDGWRILMLLLEAVRGGKKLDPNKEGMINVVGFGALMLFMLFITYKDILRLFQ
                     "
     gene            3806..4861
                     /gene="ispG"
                     /locus_tag="L3M60_003163"
                     /gene_synonym="gcpE"
     CDS             3806..4861
                     /gene="ispG"
                     /locus_tag="L3M60_003163"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBN6227795.1"
                     /translation="MSYERRKTREVSVGTVKIGGENPISIQSMTNTDTRDADATIAQI
                     KRLEEAGCDIVRVAVPDIKAAKNIAKIKSSVNIPIIADIHFDYKLALEAIEQGVDGIR
                     INPGNIGSIERVKMVVEKCKERNLKIRIGVNGGSLEKELLKKYGSATAEALVESAMGH
                     IKILEDLDFHNIVISLKSSDIYKTVDAYELISKKVDYPLHIGITESGSVHKGTIKSSI
                     GVGALLLKGIGDTVRISLTGDPVEEVIVGKQILRSLGLLNDKIKVISCPTCGRCNIDL
                     ISVVNEVEEKIGSMEKNITVAIMGCAVNGPGEAREADIGIAGGKGEGLLFKKGEIVRM
                     IDGNKLVDELLEEIEKL"
     gene            5209..6033
                     /locus_tag="L3M60_003164"
     CDS             5209..6033
                     /locus_tag="L3M60_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227796.1"
                     /translation="MRYKKISSLPILVVALLITGTTTTFSLSTTNSEKTSVYLASTVS
                     TITKKVSDIQSSKGLVYNENIPLFINNSKIQYDDTPYHWPSNVISLTNSSEKAIMDYE
                     ITCLAYDKNGKPLELYWDAQNVAADGEVGSVGFSPAGVDYGIVTGISPVSPKSYSHTY
                     RKMQQSPPQDIISMFEKQQGKAWVENWLKEWKQMEKEYAKQNAIAPGKNQNDAFLLFD
                     KWKQSTGEHGVKYIISCVKQVTFNDGSVWKNSAYENWLKSFQGKEVSNSVLENYYK"
BASE COUNT         2440 a          611 c         1138 g         2073 t
ORIGIN      
        1 tagaagtatt tcaagatgag aaaggagaaa atactgaaaa ataattattc tattataatt
       61 tgccaaggtt ttgtctattt ttcagtttaa atagtatatt acatatgaaa aagaaaaaca
      121 atttaaatta taattatgta tttgtagtct atgatgtagg agaaaaaaga gtaagtaaag
      181 tttttaaagt gtgtaaaaaa tatctctcgc attttcaaaa atcagttttt agaggtgaaa
      241 taactccatc taagattatg agcttaaaac gtgatttaga aaatattata gataaagata
      301 atgattttgt atgtataata aagttgatga attctaatac atttgaagaa gagatattag
      361 ggaataatag taattgtgaa aatatgatat tataaatcta attactatat ttgtaaaaaa
      421 taatagctta ttaaagagta gtaaaattgg gatataattt atgatagaga ctgcaagtag
      481 atttttgtaa aaaggattta cttgcagtat ttttgttatg tataactgtt aacattagtg
      541 tacaaaacat tatcaatagc atttttctat aatgaagaaa tactattata atgggaaaat
      601 tttattaata ctaggtaata atatatttac acaagtctat atatattata atttttcatt
      661 atttctaata ataagattct ttttgcattt atttaatatt attttatcaa gaataaaaga
      721 ttaaactaat tttaattatt ttaattgttc attctaaaat agtatagttc taactttcta
      781 tagtatccat catagccctt gtctcttatt ataatcaagt actttaaata tataataaaa
      841 tctttcttat atatctgcaa atataataat agtatattac tttaatttag cgccaataaa
      901 aatttttata tgttaataat aatgcatcat atgtgttata gaccaatcat ttagtatagt
      961 attaattttt catattagaa ggataaaatt atttgacact taatcaggtg attatttaac
     1021 caaatagatg aatattttcc aaaatagata aaatgtgata gaatatattt tgcgaggtga
     1081 taacatgatt tcatacgctc cattatggaa attgttaatt gacagaaaaa taaagaaaat
     1141 ggaatttgta aatatatcag gtataagtat ttctgtttta ggcagactag gaaatgataa
     1201 atctgtttct atggatacaa tggagaagat atgtttagca ctagactgta aaatagaaga
     1261 tgttgtggaa ataaaaaagg gcatttaatg atactagaat attttataat attccacaaa
     1321 atacaaaaaa taatagatta attttaatct gtggaataat atatgaaatt tctatgaata
     1381 gtaaaaataa ttgttttagt agtgttataa tgttttgata gggtatattt attattagaa
     1441 aggattatta tatgaaaaag atatcaattt taggttctac aggctctata ggtaaacaaa
     1501 ctttggatgt ggttagagaa catagagata agtttgagat agttgcaata tcagctaaca
     1561 gtaatattga attattgcta gaacaaatag tagagtttaa acctaaatat gtaacagttt
     1621 ttgaggaaaa taaagcattg aaattaaaag aaatgttgcc taaaaatata gaaatagaag
     1681 tcttagcagg aatggaagga ttaaaaataa tatcatcact tgatgaagtg gatgtccttt
     1741 taactgctgt agtaggtatg ataggattag tcccaactct ttgtgctata aagaagggaa
     1801 tagacatagc attagcgaat aaagaaacgt tggtaaccgc tggtgaactt gtaatgaaag
     1861 aagcagaaaa atacaatgta aatattcttc ctgttgatag tgaacatagt gctatatttc
     1921 aatgtctaaa tggagaaaat aaaaaaaata tagaaaagat aatacttaca gcatctggtg
     1981 gtccatttag aggaaagaaa aagggagaac ttgtaaatat aactaaaaat gaagctttga
     2041 agcatccaaa ttggagtatg ggaagaaaaa taagtattga ttcttcaacg cttatgaata
     2101 aaggacttga agtgatagaa gctagatggt tgtttggggt agaacaagaa aatattgatg
     2161 tagtagttca cccacaaagc ataattcatt caatggtgca gtatgcagat agctcaataa
     2221 ttgctcaatt aggatgtcca gatatgagac taccaataca gtatgctctt acatatccag
     2281 atagaatgga aagtagcttt gaaagaatga atttttcaaa atttagtact ttaacttttg
     2341 aagaaccaga tttagaaact tttccatgtt taaaattggc atatgagtgt ttaaaaatgg
     2401 gaggaactta ttcttctgtt ttaaattcag caaatgaagt tttagtaagt gaatttttag
     2461 aggataaaat tggtttctat gacataccat attacataga gaagacttta gaagttcata
     2521 gtagcataag tgaaccaaca ttagaagaaa tcttagaaac agatagatgg agtagagctt
     2581 atgttgcaaa tctgataaaa aaataggaag gtgaatttat gactataata gccgcattaa
     2641 tactatttag tataattgta ttaatacatg agttaggaca ttttatattt gcaaaaagaa
     2701 gtggtatcaa agtaaatgag ttttctatag gtatggggcc taagatatat agtgtaaaaa
     2761 aagatacaga gtattctata agagcacttc caattggtgg atatgtcagt atggaaggtg
     2821 aagatgaaga gcaaataagt ccaaattctt ttggaaacaa gtctatatta caaagattta
     2881 gtactattgt agcaggtcca atattcaata taatattagc agcaatactt ttagtaccag
     2941 tatttttgta tataggttct ccaacgacta aattaggaaa gataatgcca gatactcctg
     3001 cacaggctgt agggcttcaa gtaggagata agataaataa aattaatggt aattctgtta
     3061 agacttggga tgaagttgca aatataataa acacttcttc tggtggagaa ttaaaactta
     3121 gtataactag agatggaagt gataaagttg taaatgtaac acctaaaaat aacaatggaa
     3181 agtatgaaat agggattcaa ccacaaaggg aaaaagactt ttgggggtca atagtaaatg
     3241 catgtaaaac tactgtagat atgacaaaac agatgctaac atttctaggt caaatgatta
     3301 caggacgtgt accaggaggg attggcaatg ctgtagcagg accagtaggt gttattggta
     3361 tggtatcgga tgctgctcgt acaggcatca taaatgtagt gtatttagca gctgtcataa
     3421 gcttaaattt aggtatagta aatctattac caataccagc gcttgatgga tggagaatac
     3481 ttatgctatt actagaagct gttagaggtg gtaaaaaact tgacccaaat aaagaaggca
     3541 tgataaatgt tgttggtttt ggagctttaa tgttatttat gctttttatc acatataaag
     3601 atatactaag attatttcaa tgatttagtt ttttagagat tttaagaaaa taggctatgt
     3661 agttttaaat aagaataaat taatttttat gtatgaaatt tattcttatt taactagata
     3721 gctttttaat ttataatata catgtaggtt ataatatttt aatatttaaa gagattcaat
     3781 ataaaataat atatagaggt ggaatatgag ttacgagaga agaaagacga gagaagtaag
     3841 tgtaggaact gtaaaaatag gaggagaaaa tcctataagt atacaatcta tgacaaatac
     3901 tgatacaaga gatgcagatg ctacaatagc tcaaataaag agactagagg aagctggttg
     3961 tgatatagtt agagttgctg ttcctgatat taaagctgct aagaatatag caaagataaa
     4021 gtctagtgta aatataccaa ttattgcaga tatacatttc gattataagc tagcccttga
     4081 agctatagaa caaggtgtgg atggaataag aataaatcct ggtaatatcg gaagtataga
     4141 gagagttaag atggtagttg agaaatgcaa ggaaagaaat ttaaaaatta gaattggagt
     4201 caatggaggt tctttagaga aagaactttt aaaaaagtat ggctctgcaa ctgctgaagc
     4261 attagttgaa agtgcaatgg gacatattaa gattcttgaa gacttagatt ttcataatat
     4321 agttatatct ctaaaatcat ccgatattta taaaactgtt gatgcatatg aattaatatc
     4381 aaaaaaagtt gattatcctc ttcatattgg aattacagaa tctggaagtg ttcacaaagg
     4441 aacaataaag tcttctatag gggtaggagc acttcttctt aaaggaattg gagatactgt
     4501 tagaatatct ttaacaggtg accctgttga agaggttatt gttggtaaac aaattttaag
     4561 aagtttggga cttttaaatg ataagattaa agttatatca tgtcctacat gtggtagatg
     4621 taatatagac ttaattagtg ttgtaaatga ggttgaagaa aagattggta gtatggaaaa
     4681 aaatatcact gttgcaatta tgggctgtgc tgtaaatgga cctggtgaag ccagagaagc
     4741 agacataggt attgctggag gtaaaggtga aggcctttta tttaaaaagg gtgaaatagt
     4801 gagaatgatt gatggtaaca aattagttga tgagttgcta gaagagattg agaaattata
     4861 gtatacaatt tagatatata ttaattgact agatttttaa atacttttgt aatgagtttt
     4921 tatttacagt atttaattat ctagtcattt tttattttat aaacttcatt aaatgtaaga
     4981 aacttatact ctaatttgac tatatatgta tgagttaaat tatattgtaa atttataaaa
     5041 ttaaaaaaag atttaatata gtgaaaattt ccatcaatag tattgaaact tgatagtaac
     5101 tatgatagga tatagttact agaacatctg gtaaattatg ttaacataga ttttaatgtt
     5161 ttggtaaatt gttttattat aagttaatag aagaaggggt gaagaactgt gagatataaa
     5221 aaaatatcta gcttacctat tttagttgtt gctttgctga ttactggaac gacaacaaca
     5281 ttttctctta gtacgaccaa ttcagaaaaa acaagtgtat acttagcaag tacagtaagt
     5341 actatcacaa agaaagtatc agatattcaa agtagtaaag gtcttgttta caatgaaaat
     5401 attccattat ttataaataa tagcaaaata cagtatgatg atactccata tcattggcct
     5461 agcaatgtta taagcttaac aaatagcagt gaaaaagcaa ttatggatta tgaaattacg
     5521 tgcttagcat atgataaaaa tggaaaacca cttgagctat actgggatgc tcaaaatgtg
     5581 gcagcagatg gcgaagtagg aagtgttggg tttagcccag ctggtgtaga ttatggtatt
     5641 gttacaggca tatctcctgt gtctccaaaa tcatattcac atacttatag aaaaatgcaa
     5701 caatcaccac cacaggatat aataagtatg tttgaaaaac aacaaggcaa ggcatgggta
     5761 gaaaattggt tgaaagaatg gaaacaaatg gagaaagaat atgcaaagca aaatgcaata
     5821 gcaccaggta agaatcaaaa tgatgctttt ttattgtttg ataaatggaa acaatcaact
     5881 ggagagcatg gtgtaaagta cattatatct tgtgtaaaac aagtaacatt taatgatggt
     5941 agtgtatgga aaaattcagc atatgagaat tggttgaaaa gttttcaagg gaaggaagtt
     6001 tcaaatagtg tattagaaaa ttactataag taatgtttaa tgatagtaaa ataagtacga
     6061 atttaactaa tgttaattta attaaataag aactattttg tttaaaaatg tcagtgttac
     6121 aaggaaattt ttattgtgac aaaatggcat atatacataa gattataagc atgtgtcaag
     6181 ttttgataca tgctttttta ttttgaatat atgttttcta taaatttatg attttttagt
     6241 tatgtaattt tatatgtaaa tt
//
LOCUS       DAFNNB010000176         6113 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0181, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000176 DAFNNB010000000
VERSION     DAFNNB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6113
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0181"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..666
                     /locus_tag="L3M60_003165"
     CDS             <1..666
                     /locus_tag="L3M60_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HBN6227797.1"
                     /translation="RDLNKTKKSDLNVNIIYVDNNQSYFTYNSLVMDDNRNLINDPIA
                     IVDIDNIDDSFYLSYISRCVYFNSKKLDALADISKIIEAQGVEANIQSLRAVYNEYGL
                     EINKLEKYLNSEIFTIIIIAISNLMITYNIVVSYYERNKYKLYIKKIFGYSATARSIL
                     LIISLILTNVIPIGIISTRVDLSNNIVLFGLLILVVEVIISIILDKIISNSSFNKIIK
                     GEH"
     gene            671..1288
                     /locus_tag="L3M60_003166"
     CDS             671..1288
                     /locus_tag="L3M60_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227798.1"
                     /translation="MISLINISKKFKDKSIIDKFNLEISKGEFVAITGNSGSGKTTLL
                     NIMGLLEKPNSGDIIINNIKNPNSKQIRILQRDFYGYLFQNYALIENENVESNLKIAL
                     KYQKNINRDEKINYVLESVGLGKYNKKKIYELSGGEQQRVALARVILKECEVIFADEP
                     TGNLDKKNRDIVFDILKSLNNNGKTIVLVTHDLELSEQVDKIIKL"
     gene            1477..1821
                     /locus_tag="L3M60_003167"
     CDS             1477..1821
                     /locus_tag="L3M60_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HBN6227799.1"
                     /translation="MENLPNCPKCNSEYTYEDGTLLVCPECSHEWALGLELEGDSNII
                     KDVNGNVLNDGDSVTVIRDLKVKGSSSAIKIGTKVKNIRLIHDSSDGHDIECKIDGFG
                     AMKLKSSVIKKA"
     gene            2393..3259
                     /locus_tag="L3M60_003168"
     CDS             2393..3259
                     /locus_tag="L3M60_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBN6227800.1"
                     /translation="MISIHKNIYDDKLFEELKIDCKKCFGLCCVALYFSASDGFPIDK
                     ESGKPCMNLQPDFKCSVHDNLMKRGFKGCTAYDCFGAGQKVAQVTYKGIDWMQSPELA
                     NQMSEVFLIMRQLHEMLWYLKEASVLNISDTIKSKINLRIEETEKITNMSSEQIVDLD
                     IISHREKVNSLLLQASESVMGKVKSFIKTSTLKNMKKLSKNIDLIGADLRKTNLIGAD
                     LRGRFLIAANLRNTDLSGANLIGADLRDCDIRGANLENSIFLTQLQVNTAKGDSSTKL
                     PVSLIRPKYWGK"
     gene            3372..3776
                     /locus_tag="L3M60_003169"
     CDS             3372..3776
                     /locus_tag="L3M60_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HBN6227801.1"
                     /translation="MSYQNYKCERDYVYDDYKYNKCNEYYNDYEEMTHVHEYSESVKL
                     AEECEDRHNHRAAGVTGEVIPINGGTNHVHKINDNVDFLDHFHKICVTTGPAIRIPGT
                     DKHIHLICGETTVNDGHCHKFLFTTQIEAPLV"
     gene            3952..4401
                     /locus_tag="L3M60_003170"
     CDS             3952..4401
                     /locus_tag="L3M60_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6227802.1"
                     /translation="MKSGIILEMKQINSTEYDEMLQVWESSVRATHDFLTEKDIKFLI
                     PLVEIGLKEVENIVCIKDNDIIKGFIGIDKDKIEMLFIEDKYRGNGIGKKLIKYAIDK
                     YNVKYVDVNEQNKKAVGFYIHLGFKAFDRSEIDGQGNPFPILHMKLI"
     gene            complement(4558..4935)
                     /locus_tag="L3M60_003171"
     CDS             complement(4558..4935)
                     /locus_tag="L3M60_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN6227803.1"
                     /translation="MLTPYLIFNGTCEKAFNFYAEAFGGGKTIFARLESNPNNPVMHA
                     SVTFTKYEGCIMGADTDKPVVISGMAICVVLPSRETIEEISIKLAEGGTLVQEFLPHP
                     PPHQNDGAAEVLDRYGYTWYLST"
     gene            5197..>6113
                     /locus_tag="L3M60_003172"
     CDS             5197..>6113
                     /locus_tag="L3M60_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HBN6227804.1"
                     /translation="MVNMNIIEIRSDKIYKKIMDAPINKKEDIYIYELMKPFEFKWKC
                     MNVPIVAKQKGGYDVIIASEMLGVLSPKDIDEKQKKNINMLSTDKIWNTCKETIENSI
                     NAFIKEGYDLNIKDYKYSILLANPNSPYTILSDGYWGDGGIPGYIFLSLVPNEYTINR
                     LPVLIAHECNHNIRFQFIEWNNNITLEEMMINEGIAENFAKWMFGEEMVGPWVSRTDI
                     ETLNTYIKPIIKSALKETGFQNITSYLYGDDIAKMQGYFPVGLPYCAGYACGYYMIKY
                     YLEKTNKSIIEATLLPYSEIIEAVEEFWE"
BASE COUNT         2472 a          640 c          975 g         2026 t
ORIGIN      
        1 agagatttaa ataaaacaaa gaaatcagat ttaaatgtta atataatata tgtagataat
       61 aatcaaagct actttactta taactcactt gttatggatg acaataggaa tctcattaat
      121 gacccaatag caatagttga catagataat attgatgatt ctttttatct ttcctatata
      181 tctagatgtg tatattttaa ttctaagaaa ttagatgctc ttgcagatat aagtaaaatt
      241 attgaagctc aaggtgtaga agcgaatatc caaagtttac gtgctgttta caatgagtat
      301 ggtttagaaa taaataaatt agaaaaatat ttgaatagtg agatttttac tataataata
      361 atagcaatat caaatttaat gattacctac aatatagtag ttagttacta tgaaagaaac
      421 aaatataagt tatatattaa aaaaatattt ggttattcag ctacagctag aagtatattg
      481 ttaataattt cattaatatt aacaaatgtt attccaatag gcattatatc aacaagagta
      541 gatttaagca acaatattgt cttatttgga cttcttatat tagttgtaga agtaattata
      601 tctattatat tagacaagat tatctcaaat agttcattta ataaaattat aaaaggagaa
      661 cattaaaaag atgatttcat taataaatat atcaaaaaaa tttaaagata aatctataat
      721 agacaaattt aacctagaaa tttctaaggg cgaatttgta gcgataacag gaaatagtgg
      781 ctcaggtaag acaactttac ttaatataat gggattatta gaaaaaccta attctggtga
      841 tattataata aataatataa aaaatccaaa tagtaaacaa ataagaattt tacaaaggga
      901 tttttatggt tacctatttc aaaattatgc tttaattgag aatgaaaatg ttgaaagtaa
      961 cttgaaaatt gctttaaagt atcaaaaaaa tattaatcgt gatgaaaaaa taaattacgt
     1021 acttgagtct gtaggtcttg gaaaatacaa taaaaaaaag atatacgaat tgagtggagg
     1081 agaacaacaa agggtggcgt tagcaagagt aatattaaaa gagtgtgaag taatatttgc
     1141 agatgagcct acaggtaatt tagataaaaa aaatagagat atagtatttg atatacttaa
     1201 aagtctaaat aataatggta aaactatcgt attagtaact catgatttag agctatcgga
     1261 gcaggtagat aaaattataa agttgtaata atatcaatta caataaatat gggaaagaga
     1321 ttacagctaa atcaaattca acaatactag gaatataatt taaatattaa ataaattagt
     1381 agtttgtaaa tataagtata atatgatatt ataaaggctc aaaagaaatt ttatataata
     1441 agaaatctaa aaataataaa tggaggacat ataaatatgg aaaatttacc aaattgcccg
     1501 aaatgtaatt cagaatatac ttatgaagat ggaactcttt tagtttgtcc agaatgctct
     1561 cacgaatggg cattaggatt ggaactagaa ggagattcaa atattatcaa agatgtaaat
     1621 ggaaatgtat taaatgatgg agattctgtt acagtaatta gagaccttaa agtaaaagga
     1681 agttcatcag ctataaagat tggaacaaaa gtaaaaaata tccgtttaat ccatgactca
     1741 tcagatggac atgatataga atgtaagata gatggttttg gagctatgaa gctaaagtct
     1801 tctgttatta aaaaagcgta atattataac taaactatat tgtttattat tttcaagtat
     1861 agtataaaac cataagaatc tattaagtaa taaaaattta cttatttatc tttatatttt
     1921 cttatggttt tattaaatat agattttatt gaataccaaa atattctctc ctctgcaaat
     1981 aagccaatct tatttattta aactaatata ttttgtacta tttatattaa aattgtaaat
     2041 ttaaatgcta tgttatttag ctttatatag ttaatttaca aatttctgat tttataaatt
     2101 gtatttatta tatctttaat gctaaaatca ttaaaagttt caatttctgt aaagatttac
     2161 aatttctcaa atacattatg aatatgtgaa aaatttatta ctacaatact acatttaaaa
     2221 taagtattag tataaaggta ttgttacaag tataaagaaa tttatgtata ctattggaat
     2281 aaataatacc ttaaattata attaagaata gaactgttaa ataagagatt tgattatcat
     2341 ttgattatag ataatgtact attaaaaata aagtttaatg aggagaaaaa atatgataag
     2401 tatacataaa aatatatatg atgataagtt atttgaggag ctaaagattg actgtaagaa
     2461 gtgttttgga ctttgttgtg tagctcttta tttttctgcg tcagacggat ttcctattga
     2521 taaggaatca ggaaaaccct gtatgaattt acaaccagat tttaaatgtt ctgtacatga
     2581 taatcttatg aaacgaggtt ttaagggttg tactgcatat gattgttttg gtgcgggtca
     2641 aaaagtagca caagtaacat acaagggtat agactggatg caatcaccag aactagcaaa
     2701 tcaaatgtct gaagtatttt taattatgag acaactacat gaaatgttat ggtatcttaa
     2761 agaagcatct gtattaaata tatcagatac aatcaaatca aaaattaatt taagaatcga
     2821 agaaactgaa aagataacca atatgagttc agaacagata gttgatttgg atatcatatc
     2881 acatagagaa aaagttaatt cattattatt gcaagcgagt gagtctgtga tgggaaaggt
     2941 taaatccttt atcaaaacct ctactcttaa aaacatgaaa aaattaagca aaaatataga
     3001 tttaatagga gcagacctta gaaaaacaaa tctaatagga gcagatttaa gaggtagatt
     3061 tctaatagca gcaaatttaa gaaatacaga tttgagtggg gcaaatttaa taggagcaga
     3121 cttaagagat tgtgatatta gaggtgctaa tcttgaaaat agtatatttt taacacaatt
     3181 acaagttaat actgcaaaag gagattctag tacaaagttg ccagtttcac taatacgtcc
     3241 taaatactgg ggaaaataat taaattaata cataatttta caaaatatga acttttttga
     3301 ccttgattca tattatacat tagagattct ttataagaat ttcttaatat aatataggga
     3361 gagatataaa aatgagttat caaaattata aatgtgaaag agattatgtg tatgatgatt
     3421 ataaatacaa taagtgtaac gagtactata atgattatga ggaaatgaca catgttcatg
     3481 aatattcaga aagtgttaag ttagcagaag aatgtgagga tagacataac caccgtgcag
     3541 caggagttac tggtgaagtt ataccaataa atggaggtac aaatcatgtt cataaaataa
     3601 atgataatgt agatttcctt gaccatttcc ataagatatg tgttacaaca gggccagcaa
     3661 ttagaatacc tggaactgat aaacacattc acttaatatg tggagaaact actgttaatg
     3721 atggtcattg ccataaattc ctttttacta ctcaaataga agctccttta gtataagctg
     3781 atgtgaacta ttgtagtaaa tttgctagat tatagtatat aaatgttaaa aatatataat
     3841 agataatatt taaacttaat gaggactatg atatttaacc ttcgtagtcc tttctaagtt
     3901 ttgtataaag aagtgttata caattaaaag tgatataggg aggaaatttt tatgaaatct
     3961 ggaataattc ttgaaatgaa acagataaat tcaacagaat atgacgaaat gttgcaagtc
     4021 tgggaatcgt cagtaagagc aactcatgat tttttaacag aaaaagatat taaatttttg
     4081 ataccattag ttgaaattgg acttaaagaa gtagaaaata tagtttgtat aaaagataat
     4141 gatataataa agggctttat aggaatagat aaagataaaa ttgagatgct ctttatagaa
     4201 gataaatata gaggaaatgg tattggtaaa aaattaataa aatatgctat agataaatac
     4261 aatgtaaaat atgttgatgt aaatgagcag aataaaaaag ctgtaggatt ttatattcat
     4321 ttagggttta aagcgttcga taggtctgaa atagatggac aaggtaaccc atttccaata
     4381 ctacatatga aattaattta aagtgatact tctcacttat tcgcaaaaat atttacttaa
     4441 attttttata caaataaaag tcgtgacagt cttaggacac gactttttta ctacactatt
     4501 aaattgaaat ttatctgttc tatattatga ttattagatg tagttttttt attttaacta
     4561 tgtacttaaa taccaagtat acccatacct atcaagtact tcagcagcac catcattttg
     4621 atgtggtggt gggtgtggta aaaattcttg tacaagcgta ccaccctcgg caagttttat
     4681 agatatttct tctatcgttt ctcgagatgg tagaacaaca caaatcgcca tgccagaaat
     4741 tacaacaggc ttgtctgtat ccgcacccat tatacaacct tcatattttg tgaaagtaac
     4801 acttgcgtgc ataacaggat tgtttggatt gctttctaat cgtgcaaata tagtttttcc
     4861 tcctccgaaa gcctcagcat aaaaattaaa tgctttttca caagtaccat taaatattaa
     4921 atatggagtt agcatgacaa atatatcctc cttgatatag aataatttat agttgcacct
     4981 gataatctaa attctgattt agtaaattgt atcataggga aaccttgatg tatataattt
     5041 tttattatta gattatacaa atctattaat ttaaaaaaat gttatatgta aggaaggttt
     5101 ataggataaa atataataaa aagagtaagg tgacattata aaaaatgtat attgacattg
     5161 acgctacgta attgtatata ctttgtctta aaggagatgg taaatatgaa tattatagaa
     5221 attcgctcag ataaaatata caagaagata atggatgcac caataaacaa aaaagaagat
     5281 atatacatat atgaattgat gaagcctttt gaatttaagt ggaagtgtat gaatgttcca
     5341 atagttgcta aacaaaaagg cggatatgat gtaattatag caagtgaaat gttaggagtt
     5401 ttatcgccta aggatattga tgaaaagcaa aaaaagaaca taaatatgtt atctactgat
     5461 aaaatttgga acacttgcaa agaaaccata gaaaactcta taaatgcttt tataaaagaa
     5521 gggtatgatt taaatattaa ggattataaa tattcaatat tattagctaa tccaaatagt
     5581 ccttatacaa tattaagtga tggatattgg ggtgatggtg ggattcctgg atatatattt
     5641 ctatcattgg ttcctaatga atatactatc aatagattac cagtattaat agcacatgaa
     5701 tgtaatcaca atattagatt ccagtttata gagtggaata ataatataac attagaagaa
     5761 atgatgataa atgaaggtat tgcagaaaat tttgcaaaat ggatgtttgg agaggaaatg
     5821 gtaggacctt gggtcagtag aacagatatc gaaacactaa acacttatat aaagccaata
     5881 ataaaaagtg ctttaaaaga aactggattt caaaatataa catcttatct ttatggtgat
     5941 gacatagcta aaatgcaagg atattttcca gtagggttgc cttattgtgc aggatatgct
     6001 tgtggatatt atatgattaa gtattattta gaaaagacaa ataaatcaat aatcgaagct
     6061 actttattgc cttatagtga gataatagaa gcagtagaag agttttggga ata
//
LOCUS       DAFNNB010000177         6112 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0182, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000177 DAFNNB010000000
VERSION     DAFNNB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0182"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            275..1666
                     /locus_tag="L3M60_003173"
     CDS             275..1666
                     /locus_tag="L3M60_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HBN6227805.1"
                     /translation="MENQQLLGTERISKLLLKYSIPAIIGMLVNSLYNVVDRIFIGNI
                     PGVGPLAITGLGVTMPIMTIILAFGMLIGIGTTTTISIKLGQGKVEEARKLIGNAMTL
                     SVITGIIIMILGILFANKILTLFGASENTLIYAKSYINIILLGTVVNLLSFSLNHSIR
                     ADGSPKISAGIMIVGCLTNIVLDWILIFGFNLGIQGAAIATVTSQALTAVLTIGYYIS
                     GKSNLRFSKSNLKLDTKLIKAVFAIGMSPFAMQLAASLVQVISNIALKTHGGDLAIGA
                     MATISSIAMVFLMPIFGINQGAQPIIGFNYGAEKYDRVKKAYLGSLAVATIILCMGMV
                     VIMLFPEAIIGIFNKDPELMNISVNGLRIYLLMLPIVGLSVTGTNFIQSIGKAKMAML
                     LSLLRQVILLIPAVLILPTFLGLQGVWTAQPVSDFIATVITGIVVFRELKRYTPKTEK
                     LNENERLNEITTE"
     gene            1773..2813
                     /locus_tag="L3M60_003174"
     CDS             1773..2813
                     /locus_tag="L3M60_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HBN6227806.1"
                     /translation="MEFTKKSKLKNMYVNMNVARMLSKINEYKGRQLLYKKQPKEILE
                     NLEKKSLVDCSESTYIGNQRDSSNFNLEKLISNEVTPRSREELSIVEYRDVVKTINSA
                     YESIPISSQTILELHGYLYKFSSTRGGSYKSDNDFIEHNLKPSEFISIDSSVNKVEKA
                     VEEICEAYNTLIEEDEIDILILISAFVLDFILIHPFKEGNIKMARVLILLLLNKNGYE
                     VGRYISLGKIFDDSSYEYYSNLNYLKASIGSEKADMNAWIEYFLETILTAYEKLDESL
                     NISDKKRQTKTSRIEKIINSTLGYFTKEDIRDLCPDIPEPTINRVFNNLRKQDKIEVV
                     ARGRSAKWKKKY"
     gene            3305..3958
                     /locus_tag="L3M60_003175"
     CDS             3305..3958
                     /locus_tag="L3M60_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBN6227807.1"
                     /translation="MKKKLLYINVNSKPEDLSSSKTVARKFINKFIEKNKDFEVEEID
                     LYKEHIPRLEYQYFEKRNSIVSEENAKNLDDKDRKELTKIRNLCDQFVSASVYVIAAP
                     MWSLSFPAPLKEYIDCIIQDGKTISFEGNDKPQGILNDIDRSMVYIQSSGGHIPWVLK
                     PVMNKGLNYVESIMKFIGIKKFDELLVDGTGTSEEERQAAIEKASEKIDGIIDGMKF"
     gene            4034..4243
                     /locus_tag="L3M60_003176"
     CDS             4034..4243
                     /locus_tag="L3M60_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227808.1"
                     /translation="MREEKSNEKYDCYWCNQENNFCVEIKDNVVMIDDGTGTLKQAIF
                     IGYKQIQINLNCSHCQNLNRIKLNL"
     gene            complement(4383..4949)
                     /locus_tag="L3M60_003177"
     CDS             complement(4383..4949)
                     /locus_tag="L3M60_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HBN6227809.1"
                     /translation="MMNNELSTIILETLNNADITSNDIPSIDLYMDQIISLIDNKLSA
                     NKRFESDKILTKTMINNYSKEGLIKPVKGKKYTKEQILQMIIIYSMKNTLTIQEIKRI
                     LHGVYEKDNFSEKDLVSCYEKFMLIKENQRKNMPDFIESNFENISINPENKDDLLIAL
                     LSLTSMADQLKNISEKLVDRYFPDIAKK"
     gene            5109..5762
                     /locus_tag="L3M60_003178"
     CDS             5109..5762
                     /locus_tag="L3M60_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBN6227810.1"
                     /translation="MYQFFLKGRDPISSLTHFIGACLSLLATIILVFQSVSLHETSLL
                     MIISVSVFGLSLIALYSASSYYHFLKGTPEQELFFRKVDHAMIYVLIAGSYTPICLNF
                     MEKKEGIIFVTAIWIVAFIGIIIKIFWMDAPRWLSTSIYLLMGWAIVFDINAFNSIPK
                     DCLRLLIMEGVSYSIGAIIYIIKKPNISPEFGFHEIFHIFIMIGSLFHFLAVLLYVL"
     gene            complement(5920..6096)
                     /locus_tag="L3M60_003179"
     CDS             complement(5920..6096)
                     /locus_tag="L3M60_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227811.1"
                     /translation="MYKYDDYVNLTNDLDLPVEIRNKCVRIVSVSENLEDIIIVHKFK
                     LYVVNEKYIKGIME"
BASE COUNT         2376 a          644 c          958 g         2134 t
ORIGIN      
        1 aatagcataa tttctttatt gtttttaaat caagtattaa ataatagttt taacttagaa
       61 aatctgggta tattactact aatatcatct atatattcac tttattttta tctattaaaa
      121 aaattttata ctagaaaagt ttggtttttt gattaaagtg gtatataata gatatataaa
      181 ttaatattta aaaagtagtt aatattaggg gagggcattt gcacctccct tgtaatatgt
      241 ggaaaattga atattagaag aaggaggaga cttgatggaa aatcaacaat tattaggaac
      301 agaacgaatt agtaaattac tactaaaata ttctatacca gctataatag gtatgttggt
      361 aaatagttta tataatgtag tagatagaat ttttattgga aatataccag gagttggacc
      421 acttgcaata acaggtcttg gtgtgacaat gccaataatg acaataatac ttgcatttgg
      481 aatgttgata ggtataggta ctactactac catatcaata aaacttgggc aaggtaaagt
      541 agaagaggca agaaaactta taggaaatgc aatgacgtta tctgtaataa caggaattat
      601 cataatgata cttggaatat tatttgcaaa taaaatactt acattgtttg gagcaagtga
      661 aaatacctta atatatgcaa aatcatatat aaatattata ttattaggta cagtagtaaa
      721 tcttctttcc ttttctttaa accactcaat aagagctgat ggtagtccta aaatttcagc
      781 aggaataatg atagtaggat gtttaactaa tatagtttta gactggatat taatatttgg
      841 attcaatttg ggaatacaag gcgctgcaat tgctacagta acttctcaag ctcttacagc
      901 tgttttaact atagggtatt atataagtgg aaaatcaaac ttacgattca gtaaatctaa
      961 tctaaaatta gatacaaagc taataaaagc agtgtttgca ataggaatgt cgccatttgc
     1021 gatgcaactt gcagcaagtt tagttcaagt aatatcaaat attgcactaa aaactcatgg
     1081 tggagattta gctatagggg ctatggctac tatttcttct attgcaatgg tatttttgat
     1141 gcctatcttt ggaataaatc aaggagcgca acctattata ggtttcaatt atggagctga
     1201 aaaatatgat agggttaaaa aagcatattt aggttcatta gcagtagcta ctataatttt
     1261 atgtatgggt atggtagtaa ttatgctttt cccagaagct attataggta tatttaataa
     1321 agaccctgaa cttatgaata tatctgttaa tggattaaga atatacttac ttatgttgcc
     1381 aattgtaggc ttatcagtta caggaacaaa ttttatacaa tctataggaa aagcaaaaat
     1441 ggctatgtta ttgagccttt taagacaagt aattttattg ataccagcag tattaattct
     1501 tcctacattc ttaggtttac agggtgtatg gacagctcaa ccagtgtctg attttatagc
     1561 tacagtaatt acaggtatag ttgtatttag agaattaaaa agatatactc ctaaaactga
     1621 aaaattaaat gaaaatgaaa gattaaatga gataactact gagtaaaaat ataaattcag
     1681 ataattgagc aagaaattgc aaaaaataca attatatgat aaaataaaac cgctgtcaag
     1741 gataagagaa attctaatta tggagtgatt ttatggaatt tacaaagaaa agcaaattaa
     1801 aaaatatgta tgtaaacatg aatgtagcta gaatgttgtc taagataaat gagtataagg
     1861 gtagacaact gctttacaaa aaacaaccaa aagaaatttt agaaaactta gaaaaaaaat
     1921 ctttagtaga ttgttctgaa tctacataca ttggaaatca aagagatagt agtaatttta
     1981 atctagaaaa attgataagt aatgaagtta cacctagaag tagagaggaa ttgagtattg
     2041 ttgaatacag agatgtagta aaaactatta acagtgctta tgagagtatt ccaatcagtt
     2101 cacaaacaat tcttgagctt catggatact tatataaatt ttcatctact agaggaggaa
     2161 gttataaatc agataatgat tttattgaac acaatttaaa acctagcgaa tttataagta
     2221 ttgattcaag tgtaaataaa gttgaaaaag ctgtagaaga aatatgtgaa gcttataata
     2281 cattgataga ggaagatgaa attgatatat taatattaat ttctgcattt gtgctagatt
     2341 tcatattgat acatccattt aaagaaggta atataaaaat ggcgagagta cttatcctat
     2401 tgttactaaa taaaaatgga tatgaagttg gaagatatat aagtttagga aagatatttg
     2461 atgatagttc atatgaatat tatagtaatt taaattattt gaaagcgtct ataggtagtg
     2521 aaaaagcaga tatgaatgca tggattgagt attttttaga gactatattg actgcatatg
     2581 agaagctaga tgagagttta aatatatctg ataagaaaag acagacaaaa actagtagaa
     2641 tagagaaaat aataaattct acccttggat attttacaaa agaagatata agagatttat
     2701 gtcctgatat tccagaacct actataaata gagtttttaa caatctaaga aaacaagata
     2761 agatagaagt tgttgcaagg ggaagaagtg caaaatggaa gaaaaaatat taaaaaatat
     2821 ttaaataact aaaatggtat agtgttttgc actataccat ttttagtatc actagcatgg
     2881 gcaataattt aatagcttaa gcaatatcaa ttttatttat tgattatttt tgaactgaaa
     2941 aaagggatta tatggatacc tcaatggtaa ttaattttgg aggagaagat ataacattac
     3001 agaatatctt aggtgtatat gtagaaaaaa taaaatataa taaacgtgtg aggtttagta
     3061 attaaaaaat tatgtagctt cgcacttttt tataaaataa agatacttag cagaataata
     3121 ttttagttga tatatttata aagaggtgct tgtttgggta aaagtataaa taataaaaag
     3181 tagaagtcat gtatataaaa atataagcta tattctataa taataattat tttataattc
     3241 aacaaaagaa tagaatgggt atatttgtga aataataata taaaataatc ttaggagtga
     3301 gcttatgaag aaaaaattat tatatataaa tgtaaattca aagcctgaag atttatcttc
     3361 aagtaaaact gtagcaagaa aatttataaa taaatttata gaaaaaaaca aagattttga
     3421 ggttgaagag atagaccttt ataaagaaca tataccaaga cttgagtatc agtactttga
     3481 aaagagaaat agcattgtta gcgaagaaaa tgctaaaaat ctagacgaca aagatagaaa
     3541 agaacttaca aagattagaa atctttgtga ccaatttgtg agtgcaagtg tatatgtaat
     3601 agcagcacct atgtggagtt tgtcttttcc agcaccactt aaagaatata tagattgtat
     3661 aattcaagat ggaaaaacta tatcttttga aggaaatgat aaaccacaag gaattttaaa
     3721 tgatattgat agaagtatgg tatatataca atcctctggt ggacacatac catgggtatt
     3781 gaagccagtt atgaataaag ggttaaatta tgtggaaagt attatgaaat ttataggaat
     3841 taaaaagttt gatgaattgt tagtagatgg aactggaact agtgaagaag aaagacaagc
     3901 tgcaatagaa aaagcatctg aaaaaataga tggtataata gatggtatga agttttagat
     3961 ataatatttt attaaaaata taaaggaaaa acctctttta atgtagaaac ttatattgaa
     4021 ggaggcgaga atcatgcgag aagaaaaaag taatgaaaag tatgattgtt attggtgtaa
     4081 tcaagagaat aatttctgtg tagaaataaa agataatgta gttatgatag atgatggcac
     4141 tggtacatta aaacaagcca ttttcatagg gtataaacaa atccaaatta atttaaattg
     4201 ttcacattgt caaaacttaa atagaataaa attaaatttg tagaatttta aaatggaact
     4261 tattgaagga attaaaaata gaaagtgtta atatttagac tgaaaattat attttataca
     4321 ataagagttt atttaaataa cattgtatct taatatttat tttcagtctt actttattaa
     4381 aattattttt tagctatatc tggaaaatat ctatctacta atttttctga aatgtttttt
     4441 aattggtcag ccattgaagt taaacttaat aaggctatca ataaatcatc tttattttct
     4501 ggatttatac tgatattttc aaaattagac tctataaagt ctggcatatt ctttctttga
     4561 ttttctttta tcaacatgaa tttttcgtaa catgaaacta aatctttctc actgaaatta
     4621 tccttttcat acacaccatg taagattctt ttaatttctt gtattgtaag tgtatttttc
     4681 atagaataaa taattatcat ctgtaaaatt tgttctttag tatacttttt tcccttaact
     4741 ggtttgatta atccctcttt gctgtaatta tttatcatgg ttttagtaag aattttatca
     4801 ctttcaaatc ttttattagc agaaagctta ttgtcaatta gagaaataat ttgatccata
     4861 tacaagtcaa tagagggaat atcgtttgaa gttatatctg cattatttag tgtttctagt
     4921 attatagtac taagttcatt attcatcata tgttacctcc ataatatata taatacacta
     4981 aattagaaaa taaaacaata ttaagacttg taaaaaaaac ataaagtggt ataattaaat
     5041 atataatata gtaatgaaaa ctatattaat gagtgtgatt tttaaataca taattgtagg
     5101 aggatgaaat gtatcaattt tttttaaagg gtagagaccc aataagcagt ttaactcatt
     5161 ttataggtgc ttgtttatct cttttagcaa ctataatttt agtttttcaa tctgtttcat
     5221 tacatgaaac ttctctcttg atgataatat ctgtatcagt atttggactt tcactaattg
     5281 ctttatacag tgcaagttcg tattatcatt ttttaaaggg tacaccagaa caagaattgt
     5341 tttttagaaa agtagaccat gcaatgattt atgtattgat tgctggttct tatacaccaa
     5401 tatgtctaaa ttttatggaa aaaaaagaag gcataatatt tgttacagca atatggatag
     5461 ttgcattcat tggcataata ataaaaatat tttggatgga tgcacctagg tggttatcta
     5521 cgagtatata tttacttatg ggttgggcaa ttgtttttga tataaatgcg ttcaattcaa
     5581 ttcctaagga ttgccttaga ctattaatta tggaaggtgt atcttattct ataggtgcaa
     5641 ttatttatat aattaaaaag cctaatataa gtcctgaatt tggatttcat gaaatatttc
     5701 atatttttat tatgataggt tctttatttc actttttagc agttttattg tatgttttat
     5761 agttttaaaa gattcgatta attgatacta aaattctaca atgaataaaa tggaactgcc
     5821 ttatatagat tatatcttcg acaataagac agttccttat tataaatatt aaattctact
     5881 tataataaaa ttttgataaa aatagtccat tcaaaatgcc tattccataa ttcctttaat
     5941 gtacttttca tttacgacat atagtttgaa tttatgtact ataattatat cctctaaatt
     6001 ctctgaaaca ctaacaattc tgacacattt gtttctaatt tctacaggca aatctaaatc
     6061 atttgttaaa ttcacatagt catcatattt atacataaaa tcatcccttt ac
//
LOCUS       DAFNNB010000178         6079 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0183, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000178 DAFNNB010000000
VERSION     DAFNNB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0183"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..734
                     /locus_tag="L3M60_003180"
     CDS             <1..734
                     /locus_tag="L3M60_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6227812.1"
                     /translation="RAIDYVKEASDYIETSDGTRITASQMCERFLFNGTMQYTLIGKL
                     SGGERRRLHLLKILMTAPNVLLLDEPTNDLDIETLKILEEYLDEFKGVVVTVSHDRYF
                     LDRICNKIFSYEGNGKIHIYTGNYSDYLIYREIQGIEFEESKKESVKKDDSITPKKEK
                     PKNDKKLKFSYNEQREFENIDSDIEKLESKIATLDESTSKFSTDFTKLQEILNEKSKL
                     EKELEYKYERWEYLNNLADEIANNK"
     gene            complement(983..1330)
                     /locus_tag="L3M60_003181"
     CDS             complement(983..1330)
                     /locus_tag="L3M60_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HBN6227813.1"
                     /translation="MSKSNDVLDIVFKITNGLEYEVDENSVVTILEKQDHKIQKFFRK
                     LKARIPKYKKIELDEYSSFVFLQIDGQRTVREIGKNLEAKYGEESHPLYERLLVFLNH
                     IEVNCHYIERLDI"
     gene            complement(1317..3248)
                     /locus_tag="L3M60_003182"
     CDS             complement(1317..3248)
                     /locus_tag="L3M60_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HBN6227814.1"
                     /translation="MNKKLPKGAYGEVSGKDYVPYITDKSRTGGNVAVLIIGIILAAI
                     FAASTTYSGMKAGLTVAAGIPGAIIGSAFVGAFARSKGILGKNLIQGMSSGGESVASG
                     FIFVLPAVILIGSQITFLEGLAVGVGGVLFGIGVAAIVHNYLIVEEHGKLMYPESMAI
                     SETLVASEAGGDSIKYMGIGFVISGFITVLTGSFLNVANNVMSLVGSKFYKWKFDIEV
                     NPLLLGIGFIVGLEVSLTMFAGSILSNFGIAPLIGYFTDMAKDGAMVWNNPAMPLNQM
                     DVGAISSSYVKYIGAGMMLCGGIIGAIKLIPTIIASIKETLKAKSSTGEGEEGSSIQM
                     ILLLGGVVIGFLAAFLISGGNIVMAIIGAIISLLLSLLFVIVAGRLTGTIGTSNLPVS
                     GMTIASLVIVTLVFVIMGWTDLEANKSLLLFGSFIVVAIAIAGGYTQSQKVTYIIGGS
                     KNEMQRYFTIASIVGVIVVVGVILLLSDQLRATGDNVQFALPQANLMSTLTSGIMSGS
                     LPWVMIIVGVFMAIVLYALNLPIMTIAIGFYLPIATTSIILVGALIRLFVELVSKTEK
                     EKEVKVSNGISLSSGLVAGGSIIGLIGIILQVTGVVTPKVPSGFAATNSMAIALLVVL
                     VVLTALPIILSKVKNNEQE"
     gene            complement(3659..5848)
                     /locus_tag="L3M60_003183"
     CDS             complement(3659..5848)
                     /locus_tag="L3M60_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HBN6227815.1"
                     /translation="MYEVNLERAMNYWMDLLDDYTNIAKIPNDFFYKDCSDEVVDEVF
                     KMNIEKHERLIQYSKVNNISIDSIIETIYGLILQKYIYEDDVVFGKTMNIIPIRVRVR
                     QSEKFIDVVRKLKRQWSRSLEYGYYLLSDIENKNHLNTKLINTIFIAKSFDLSYDYDI
                     EKKGYDLSVIVYTKRALKIKIKYKPSIYKRETIRRILNQFDYIIEQIIEDKDILIEDL
                     EFVCKEEEKVLLEDFNDNQYEVPHDKSYVDLFREQVKVTPSNIAARDENRNLTYKELD
                     EVTDKLAGYLNYIGVESEDIVAVMLPRDINIIITAIGIMKSGGAFFPIDTSNPEERLN
                     YLLEDSNAKVVITTDELKSKVVNENTIVLDINDEEMFKLEYELTEKITPSNCAYTIST
                     SGSTGRPKTIAIEHKSLVNMCYYSVKSINATENDICGIYLSFSFDAVMKQLFPYLLVG
                     ASIDIMPEEAKFDEYTVNEYCEENDITILALPTAFARLFIQNCNNNSLRVVQTGGERL
                     KGYKKRNYELYNEYGPTEFTVISTSFHVDREYGKIPIGKPISNTYAYVLDKKNKLCPI
                     GVPGELCLSGIQISRGYLNKKGLTEQVFVENPYKTCEHNKLMYRTGDLVRWLDDGNLD
                     YIGRMDNQVKIDEFRIELYEIENIINNITEIKSVVCISRTNDEGDMYICAYYVIGDED
                     SDKINERTIREYLNEHLPPYMIPTIIMRIDKIPVTPIGKVNKKALPE"
BASE COUNT         2118 a         1048 c          677 g         2236 t
ORIGIN      
        1 tgagggctat agactatgta aaagaagcta gtgactatat agaaacttca gatggtacta
       61 ggattacagc ttctcaaatg tgtgaaagat tcctttttaa tggtactatg cagtatactt
      121 tgataggtaa attatctggt ggtgaaagaa gaagactaca ccttcttaaa atccttatga
      181 cagctcctaa tgtgcttctt ttagatgagc ctacaaatga tttagacatt gaaactctta
      241 aaatattaga agaatactta gacgaattta aaggtgtagt agttacagta tcacatgata
      301 gatatttctt agatagaatt tgtaataaaa tattttcata tgaaggtaat ggaaaaattc
      361 atatctatac aggtaattac agtgattatc ttatttatag agagattcaa ggaattgaat
      421 ttgaagaatc taaaaaagaa tctgttaaaa aagatgactc tattacccca aaaaaagaaa
      481 aacctaaaaa tgataaaaag cttaaattct catataatga acaaagggaa tttgaaaata
      541 ttgactcaga tatagaaaaa cttgaatcta aaattgcaac acttgatgaa agtacttcca
      601 aattttcaac tgactttact aagcttcaag aaattttaaa tgagaagtct aagcttgaaa
      661 aagaacttga atataaatat gaaagatggg aatatttaaa taatctagct gatgaaatag
      721 ctaataataa atgatattaa aataatctta agtttaataa gttttgcttt aaatctgtac
      781 tttataaata ttttaaagag tgtgtctcaa aatgaacttt ttagtttacg agacactctt
      841 tttatatgaa atcaaatata ttttcatcat ttcaacaata taaaagaggc ttatctctat
      901 tttgagacag cctctttcat aatttttata attaccttct aatttatatg ctctaatata
      961 tctataataa ttcttattat atctatatat ccagtctttc aatatagtga caattaactt
     1021 caatatgatt taaaaatact aacaaccttt cataaagtgg atgagattct tctccatatt
     1081 tagcttctag gttcttgcct atttctctaa cagttctttg accatcaatt tgtaaaaaaa
     1141 caaaacttga atattcatct agttctattt ttttgtattt aggtattcta gcttttaatt
     1201 tcctaaaaaa cttttgaatt ttatggtcct gtttttcaag tatagtaaca acactatttt
     1261 catctacttc atattcaagt ccattagtaa ttttaaatac aatatctaaa acatcattac
     1321 tcttgctcat tattttttac tttagataaa ataataggta atgctgtaag cacaactaaa
     1381 actactaata aagcaattgc cattgaattt gttgcagcaa atccacttgg tacctttgga
     1441 gttacaactc cagttacttg tagtattata cctattaaac caatgataga accaccagca
     1501 acaagaccag aagataaact tataccatta gaaactttaa cttctttttc tttttcagtt
     1561 ttagatacta attcaacaaa taaacgaatc aatgctccaa ctaatattat agaagttgtt
     1621 gcaattggta aatagaatcc tatagctata gtcattattg gtaaatttaa tgcatataga
     1681 acgattgcca taaatactcc aacgataatc ataacccaag gtaaactacc tgacatgata
     1741 ccagaagtta atgttgacat taagtttgct tgtggtaatg caaattgtac attatcacca
     1801 gttgctctaa gttggtcaga aagtaataaa attactccta caacaactat aacaccaact
     1861 atacttgcaa ttgtaaagta acgttgcatt tcgtttttgc taccaccaat aatataagtt
     1921 actttttgag attgagtata acctccagct atcgcaatag caacaactat aaatgaacca
     1981 aataaaagta atgatttatt agcttctaaa tcagtccagc ccattataac aaacactaat
     2041 gttacaataa ctagagaagc tatagtcata ccagaaactg gaagatttga tgttccgatt
     2101 gttcctgtta aacgaccagc tactatgaca aataataaag ataataaaag agatatgata
     2161 gctccaataa ttgccataac aatgttacca ccagaaatta agaatgctgc taaaaatccg
     2221 ataactactc cacctaataa aagtatcatt tgtatagatg aaccttcttc tccttctcca
     2281 gtacttgact tagctttaag tgtttctttt atagaagcga ttatagttgg tataagtttt
     2341 attgcaccaa taatacctcc acaaagcatc ataccagcac ctatatattt tacataacta
     2401 ctagatattg ctccaacatc catttggtta agaggcattg caggattatt ccataccata
     2461 gctccatctt tagccatatc tgtaaagtat cctattaaag gtgctatacc aaagtttgat
     2521 aaaatacttc ctgcaaacat tgttaaagaa acttcaagcc ctactataaa tccaattcct
     2581 aaaagtagag gatttacttc aatatcaaat ttccatttgt agaatttgct tcctacaaga
     2641 ctcattacat tgtttgctac atttaagaat gaaccagtta atacagtaat aaatccactt
     2701 attacaaatc caattcccat gtatttaatt gaatctccac cagcctctga agcaacaagt
     2761 gtctcagata tggccattga ttctgggtac ataagtttac catgttcttc aactattaga
     2821 tagttgtgaa ctatagcagc aactcctatc ccaaataaaa ctccaccaac tccaacagct
     2881 aaaccttcta ggaatgtaat ttggcttcca attaatataa ctgctggtag tacgaaaata
     2941 aatccacttg caacagattc tccaccactt gacatacctt ggattaagtt ctttccaaga
     3001 ataccctttg aacgagcaaa tgctccaaca aaagcagaac ctattatagc tcctggaata
     3061 cctgcggcaa ctgtaagacc agctttcatt ccagagtaag ttgtagatgc ggcaaaaatg
     3121 gcagctaaaa taataccgat aatcagtaca gctacatttc caccggttct ggacttatct
     3181 gtgatgtatg gaacgtaatc ttttccacta acctcaccat aagcaccctt aggtaatttt
     3241 ttgttcataa aattttcacc ccttttcaat ttgtatatat tataacatat atacatataa
     3301 aacttaactt tttattattt tacaaatagc gagccaaaac tactaaaatt taatactata
     3361 ttattaattt ctacttttag atatttttat gctaattttc tttttatttt tcaattctac
     3421 taaaaactat taagatataa aacaaaaaat ttttttaatg taaaattaat tttgtaaaaa
     3481 aagtgcttta aaacacaaaa aagtataaaa aatacaataa tcattcttat aaataaattt
     3541 tcaccatagt aaatatatac agaaaattct tttttttaca tcaattgatt ttcttataaa
     3601 aataggtgtc ataaagaaat atatgaaatc actttacaac acctgtcata ataaaaaatc
     3661 actctggtag tgctttttta tttacttttc ctattggtgt aactggtatt ttatctatcc
     3721 tcattattat tgttggaatc atatatggag gtagatgctc gtttaaatat tctctaatag
     3781 ttctttcatt tatcttatca ctatcttcat caccaattac ataatatgca catatataca
     3841 tatccccctc atcatttgtt ctcgatatac agacaacact tttaatttct gtaatattat
     3901 ttattatatt ttcaatctca tatagctcta tcctaaactc atcaattttt acttgattgt
     3961 ccatccttcc aatataatct aaatttccat catctaacca ccttactaaa tcaccagttc
     4021 tatacattaa cttattatgt tcacaagttt tgtaaggatt ctctacaaat acttgctctg
     4081 ttagtccttt tttatttaaa tatcctcttg atatttgaat gcctgatagg cataactctc
     4141 ctggaactcc aattggacat agtttgttct ttttatctaa tacatatgca taagtattag
     4201 atataggttt ccctattggt atttttccat attctctgtc aacatgaaaa gaagtcgata
     4261 taacagtaaa ttctgttggt ccatactcat tatacagctc ataattacgc tttttatatc
     4321 cttttaatct ttctcctcca gtctgtacaa ctctaagtga gttattattg caattttgta
     4381 taaataacct tgcaaacgct gttggcaacg ctagtatagt tatatcattt tcctcacaat
     4441 attcatttac tgtatattca tcaaatttag cttcttcagg cataatatct attgatgcac
     4501 ctacaagcaa ataagggaat agttgtttca ttacagcatc aaaactgaaa cttaagtata
     4561 taccacatat atcattttct gtagcgttta tagattttac actatagtaa cacatattta
     4621 ctaaactctt atgctctata gctatggtct ttggcctacc agttgaacct gaggtagata
     4681 tcgtatatgc acaatttgat ggtgttattt tttcagttaa ttcatattca agcttaaaca
     4741 tttcttcatc atttatatcg agtactatgg tattctcatt tacaacctta ctttttagtt
     4801 cgtctgtagt tatcactact tttgcattac tatcttcaag aaggtaattc aatctttctt
     4861 ctggattgct tgtgtctatt ggaaaaaatg ctccacctga cttcataatc cctatagctg
     4921 taataattat gttaatatct cttggtaaca ttacagctac tatgtcttca ctttccactc
     4981 ctatataatt taaataacct gcaagtttat cagttacctc atccaattct ttatatgtta
     5041 aatttctatt ttcatcccta gcagctatat tactaggtgt taccttaact tgctccctaa
     5101 ataaatcaac ataactttta tcatgtggta cttcatattg attatcatta aagtcttcca
     5161 ataatacttt ttcttcttcc ttacatacaa attctaaatc ttctattaaa atatctttgt
     5221 cttctattat ttgctctatt atataatcaa attgatttaa aattcttcta atagtctctc
     5281 ttttatatat agatggttta tactttattt ttattttaag cgctctctta gtatatacta
     5341 ttacagataa atcatatccc tttttttcta tgtcataatc atatgataaa tcaaaacttt
     5401 tagctataaa tattgtgttt ataagcttag tatttagatg atttttattc tctatatctg
     5461 ataataaata gtatccatac tctaaagacc tgctccactg cctttttagc tttcttacaa
     5521 catctataaa tttttcactt tgtctaactc gtactcttat tggaattata ttcattgttt
     5581 taccaaaaac tacatcatct tcatatatat acttttgaag tataagacca tatatagttt
     5641 ctatgataga gtctatgcta atattgttca ctttagaata ttgaattaac ctttcatgtt
     5701 tctcaatatt catcttaaaa acctcatcta ctacttcatc agaacaatct ttataaaaaa
     5761 aatcatttgg aatttttgct atattcgtat aatcatctaa caaatccatc caataattca
     5821 tagctcgttc taaattaact tcatacatat caaaattccc ccttactact aagtctatat
     5881 aaaaaattct tttggaattt tatttttcac aatatataat atattaataa tacatatgca
     5941 agctctaaaa gcatgagctt gcataaaatt acacctatta ttatggctac taaattcgta
     6001 atatttacaa tgtttaaaat atattataac acatatatga ttttattttt ctacatttat
     6061 ataatatata aattacaaa
//
LOCUS       DAFNNB010000179         5962 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0184, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000179 DAFNNB010000000
VERSION     DAFNNB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0184"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            432..1355
                     /locus_tag="L3M60_003184"
     CDS             432..1355
                     /locus_tag="L3M60_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HBN6227816.1"
                     /translation="MGSGKVGTSLANYFLSKGYCVSGFYGRNQLSLLESTNLTKTKIY
                     SNLKDIIYENDILFITTSDDSIEIIDKKLSKFNLKHKYICHTSGSLKSSILFCSKKAG
                     ALIYSIHPIFAFSNKNTDLKKMKDICFSIEGNNEQGDLVIQNFIDSLGNQFFIRDKDT
                     SSTYHLANVLVSNLVLSLLDIGVSYFIKLGLSEEESLKAITPLVEKNIENILDNGFTK
                     ALTGPVSRGDITPIMKHLSVLDKKDEEIYKILSLNLLKITALGNDNLLKCTKNITIEN
                     AVENLISKSEKYKKIYKLLGGKNDEKYYTDF"
     gene            1330..2157
                     /gene="panB"
                     /locus_tag="L3M60_003185"
     CDS             1330..2157
                     /gene="panB"
                     /locus_tag="L3M60_003185"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBN6227817.1"
                     /translation="MKNTILTFKNAKYEGKKLSMLTAYDYSMAKIMDECDINGILIGD
                     SLGMVIKGEENTLSVTIDEIIYHTKAVKNGVKNALIVSDMPFLSYHVSIEDTVKNAGR
                     LVKEGGAHAVKLEGGSNIIKQIESIVNAQIPVMGHLGLTPQSVNSFGGFKIQGNTSET
                     ARQLIEDAKLIEKAGAFSIVLEGVPTKIAEMVTNSISIPTIGIGAGINCDGQILVYQD
                     MLGMFGDFVPKFVKQYANIGDIMRDSIKNYILEVKTGAFPQEKHSFSINESELEKLY"
     gene            2176..3024
                     /locus_tag="L3M60_003186"
     CDS             2176..3024
                     /locus_tag="L3M60_003186"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBN6227818.1"
                     /translation="MLVKEIKLLRNIIKDWRKDGYSIGLVTTMGFLHEGHQSLIKKAV
                     KENDKVVVSVFVNPTQFGPNEDFNSYPRDIERDFKYCMDSGATVVFNPSPEEMYLKGN
                     CTTINVSGLTDFLCGAKRPIHFGGVCLVVSKLLNIVTPDKAYFGEKDAQQLAVIKRMV
                     KDLNIDTEIIGCPIIRENDGLAKSSRNTYLSEEERKSALILNRSLSLAKEELVKGNLN
                     PKNIKELITAKINSEHLAKIDYVEIVDSETLQPVGQIENSVLVAIAVFIGKTRLIDNF
                     TFELNI"
     gene            3387..3569
                     /locus_tag="L3M60_003187"
     CDS             3387..3569
                     /locus_tag="L3M60_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227819.1"
                     /translation="MFKDKIDECIHIMTAYIASLKEYYSFIETQIGDFIKKYGEDTVE
                     LCLHRIMILLCECGLA"
     gene            3874..4788
                     /locus_tag="L3M60_003188"
     CDS             3874..4788
                     /locus_tag="L3M60_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2935 domain-containing protein"
                     /protein_id="HBN6227820.1"
                     /translation="MIDNQKYVILSLELHLFFSRIMKEHALFLEAGFTSKDYNLAMEA
                     DHYKKQFEDLLSYTVSASNGIIRPDILYSEELVTTLTSAAEQKTEEFTGIEINKNITT
                     RELNLQSGVDPEVGQDLVNYVAQLNSDAIRLLDGLINLKERVLDGVLSCTLFTSNYPL
                     LLEHIIHEANLYRSYIVDLENKIDIESKNAKEIELFWDHIMMEHALFMRGLLDPSEGE
                     LINTSNDFALKFNKLIEKANEMTDSNIKSITEETLNETVEFKDFKEEGASGIEQCKIK
                     SIILPLLADHVLREANHYIRILESYKNM"
     gene            5170..5817
                     /locus_tag="L3M60_003189"
     CDS             5170..5817
                     /locus_tag="L3M60_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HBN6227821.1"
                     /translation="MIRQTPLEKRYDKLGPHIVTALKKRYFDAYYCKTRNEALQQILD
                     LIPQNHVVSWGGSETLKEIGVQTAVREKGFKVIDRDLAKTPEERTEIMRQALLCDTFL
                     MSSNAISEDGQLFNIDGNGNRVAAMIFGPKNVIVVAGMNKIVKNIDDAIQRTRTIASP
                     AVVQRFQDVNTPCYINGSCIDCTSPESVCAYMVTTRISRPAKKIKVILVGENLGL"
BASE COUNT         2378 a          677 c          894 g         2013 t
ORIGIN      
        1 tattaaattt taaccttgta ctaatattga caaattgtag ataaaaataa attatatatt
       61 ttattattgt tttaaatttt tataaaaaac taatattatt gatagtattg aagtaaaaaa
      121 gaattaattt ctaaaaatat attaattttt taataaactt aatcactttt aatagatata
      181 gtaaaacaaa aaaattatat tttataaata tcataatttt tttactgata ttctattata
      241 taaattttat agaattaatt gtcaattttt tatgaaaatg gtaatatatt tatggatggt
      301 atcttgttat aagaactata ctttaaatgt ttttaattat tttgtcttat gtaactcata
      361 tcctcaggaa tgagattaga aattaagatg ttgtatcaca aagtggaggt gatactatta
      421 aaataggttt tattggttct ggaaaagttg gaacatcttt agctaattat tttttgtcaa
      481 aaggttattg tgtatcaggc ttttatggaa gaaatcaatt atctttacta gaatcaacaa
      541 atttaacaaa aacaaagatt tacagcaatt tgaaagatat aatttatgaa aacgatatat
      601 tatttattac aacatcagat gattctattg aaattataga caaaaaactt tccaaattta
      661 atttaaaaca taaatatatt tgtcacacaa gtggctcttt aaaatctagt atattgttct
      721 gttcaaaaaa agcaggtgca ttaatttatt ctatccatcc tatatttgca ttttccaata
      781 aaaatactga cttaaaaaaa atgaaagaca tttgtttttc catagaaggt aataatgaac
      841 aaggtgattt agttattcaa aatttcatag atagtttggg aaaccaattt tttataagag
      901 ataaggatac ttcatctact tatcatttag ctaatgtttt agtttcaaat ttagtattat
      961 ctttactaga tataggagta agttacttta tcaaattagg tttatctgaa gaggaatctt
     1021 taaaagccat cactccttta gttgaaaaaa atatagaaaa tatacttgat aatggcttta
     1081 ctaaagctct tactggtcct gtttctcgtg gagatataac accaataatg aaacatctat
     1141 ctgtattgga taaaaaagat gaggaaattt ataaaatact ctccttaaat ctactaaaaa
     1201 taactgcctt aggaaatgat aacttattaa aatgtacaaa aaatattact atagaaaatg
     1261 cagtagaaaa tttaataagt aaatctgaaa aatacaagaa gatttacaaa cttttaggag
     1321 gaaaaaatga tgaaaaatac tatactgact tttaagaatg ctaaatatga aggaaaaaaa
     1381 ctatctatgc ttactgctta tgattattca atggcaaaaa taatggatga atgtgatata
     1441 aatggcattt taataggaga ttcgttggga atggtaataa agggtgaaga aaacactcta
     1501 tctgttacta tagatgaaat tatatatcat acaaaggctg taaaaaatgg cgtaaaaaat
     1561 gccttaatcg taagtgatat gcctttttta tcttaccatg tttctataga agatacagtt
     1621 aaaaatgctg gtagacttgt aaaagaagga ggagctcatg ctgtaaagtt agaaggtggc
     1681 tcaaacatta taaagcaaat agaaagcatt gtaaatgccc agatacctgt aatgggacat
     1741 ttaggattaa ctcctcaatc agtaaattca tttggtggat ttaagattca aggaaatact
     1801 agtgaaacag caagacaact tattgaggat gctaaattaa tagaaaaagc aggtgcattc
     1861 tctattgtcc ttgaaggagt gcctacgaaa atagctgaga tggtaactaa ctctatttca
     1921 attcctacaa taggtatagg agcaggaatt aactgtgatg gacaaatttt agtttatcaa
     1981 gatatgttgg gaatgtttgg agattttgtt cctaagtttg taaagcagta tgcaaatata
     2041 ggagatataa tgagggattc aattaaaaat tacatactag aagttaagac aggtgcattc
     2101 ccacaagaaa aacacagctt ttcaataaat gaatctgaat tagaaaaatt atactaatat
     2161 taaggatggt acaaaatgct tgtaaaagaa ataaaattac taagaaatat aatcaaagac
     2221 tggagaaaag atggctattc tatagggctt gttacaacaa tgggattttt acatgaagga
     2281 caccaaagtc ttataaaaaa agctgtaaaa gaaaatgata aagtagtagt gagcgtattt
     2341 gtaaatccaa ctcaatttgg accaaatgaa gattttaata gctatccaag agatatagag
     2401 agagatttta aatactgtat ggatagcggt gcaacagtag tctttaaccc atcaccagaa
     2461 gaaatgtatt taaaaggtaa ttgtactacc atcaatgttt ctggacttac agattttctt
     2521 tgtggagcta aaagacctat tcattttgga ggtgtctgcc tagttgtttc aaagcttcta
     2581 aatatagtaa cacctgataa agcttatttt ggagagaagg atgcccaaca attggcagta
     2641 attaaaagga tggtcaagga tttaaatata gatacagaaa taataggttg ccctataatt
     2701 cgtgaaaatg atggattagc aaaaagttca agaaatacat atctctctga ggaagagcga
     2761 aaatctgctc taatcttaaa taggagctta agtttagcaa aagaagagct agtaaaaggt
     2821 aatctaaatc caaagaatat aaaagaatta ataacagcta aaataaattc tgagcactta
     2881 gcaaaaatag actatgttga aattgtagat agtgagacat tacaacctgt tggacaaata
     2941 gaaaattctg tacttgtagc aattgctgtg tttataggaa aaaccagact aattgataat
     3001 tttacatttg agttaaatat atagtctgca attttatata atacatctaa aaaataataa
     3061 attgcttaga aaataataga ttacaataac taaaatttta aaactaaatt ccttaaaaat
     3121 atgattaatt gtaattatat atgtatacta ttattacact tatattaatc tatattaaac
     3181 ctctactaat ttaagtaaac ctttaactgg ataatttttc ctttaaaaat gaaaatttat
     3241 ccagttttgc gattataata accagtttta catatccact tgtaaaatat atctaagcaa
     3301 tatatcatac tatatagatt tgtgagtgaa ataatatctg ctatttactg aatattttta
     3361 tttttcgttt tttaggggga ttagttgtgt ttaaagataa gattgatgaa tgtattcata
     3421 taatgactgc atatattgct agtttaaaag aatactattc atttatagaa actcaaattg
     3481 gtgattttat taaaaaatat ggagaagata ctgtagagtt atgtctccat agaataatga
     3541 ttctactatg tgaatgtggt ttagcataaa attaaataca ttaaagtggg taagaagagt
     3601 cctatgtaaa actcctctta cccactttaa tatgtagact tagtttaatc taaataacta
     3661 agttataaaa tcatattata atttataata agaagcaggt taataaatca aatttgtttt
     3721 ataatccaac ataaatatta agaaaaagga tgtaataaga ttcttgttga tattagtgtc
     3781 aattgaattt agttaattta tttataatat acataaattt aaatattacg aatagagtat
     3841 tataaatagg atactttcta aagggaggaa attatgatag ataatcaaaa atatgttatt
     3901 ttatcactag aattacactt atttttttca agaattatga aagagcatgc tcttttttta
     3961 gaagcaggat ttacaagtaa agattataat cttgctatgg aagctgacca ctataaaaag
     4021 caatttgaag atttattatc atacacggtt agtgctagta atggtataat tagacctgat
     4081 atattatatt cagaagaact tgtaactact cttacatcag ctgcagaaca aaaaacagag
     4141 gagtttacag gaatagagat aaacaaaaac ataactacaa gagaattaaa tctacaaagt
     4201 ggtgtagacc cagaagttgg tcaagactta gttaactatg tagctcagct taactctgat
     4261 gcaataagat tacttgacgg acttattaat ttaaaggaaa gagtcttaga tggagtacta
     4321 tcatgtactc tatttacctc aaactatcct ctacttcttg aacatataat acatgaagca
     4381 aatttatatc gttcttatat agttgacctc gaaaataaaa tagacattga gtcaaaaaac
     4441 gctaaagaaa tagaattatt ctgggaccat attatgatgg aacacgctct atttatgaga
     4501 ggattattag acccttctga aggtgaacta ataaatactt caaatgattt tgctttaaaa
     4561 tttaataaat taattgaaaa agcaaatgaa atgactgatt ctaatattaa gagtattaca
     4621 gaagaaactc taaatgaaac tgttgaattt aaagatttta aagaagaagg agcatcagga
     4681 atagaacagt gtaagataaa atctataata ttaccacttt tagcagacca tgttttaaga
     4741 gaggctaatc attatattag aatattggag agttataaaa acatgtaatt aaaaaatact
     4801 tttaaaacaa tatttagata aaataaaggt acttcaactg tacctttatt tttttatacg
     4861 cttatagaat ttaaacaagt tatttataaa acaaaatcat aatcatctat aatccacaaa
     4921 tttattttat attaaataaa aagtattatt aattatattt tataaatatc tttttattca
     4981 attatttgtt aaaaactaat agtaaatctt atactaattg gacatttatg ttataactat
     5041 attgtaacta tttttataat cttaggaaaa tattaattgt aaaattaatg ttatacaata
     5101 atatatcgta taattttaat taacataata gttacaacat taaaatttaa atatgggagt
     5161 gattttagta tgataagaca aacaccatta gaaaaacgat atgacaagct aggtcctcat
     5221 attgtaacag cactcaaaaa acgttatttt gatgcttatt actgtaaaac tagaaatgaa
     5281 gctttacaac aaattttaga tttaattcct caaaatcatg ttgtatcatg gggaggctct
     5341 gaaacgctta aggaaattgg tgtacaaact gctgttagag agaagggatt taaagttatt
     5401 gatagagatt tagcaaaaac tccagaagaa agaacagaaa ttatgcgtca agcactttta
     5461 tgtgatactt ttttgatgag tagcaatgca attagtgaag atggacaact atttaatatt
     5521 gatggaaatg gtaatcgtgt agctgcaatg atatttggtc caaaaaatgt tatagttgta
     5581 gctggtatga ataaaattgt aaaaaatatt gatgatgcaa tacaaagaac tagaacaatt
     5641 gcatctccgg cagttgttca gcgttttcaa gatgtcaata caccttgcta tattaatgga
     5701 agttgtatag actgcacaag tcctgaaagc gtttgtgcat atatggttac tacaagaatc
     5761 agtcgtccag caaaaaaaat aaaagtaata ttggtggggg aaaatcttgg tttataaaat
     5821 atacatacat ataaatagct ctataaataa taaaatcata tgaaataact tattcctaaa
     5881 agtggtagaa tgaaagccac tctgcaagac tttcatttta ccacttttac attcttttaa
     5941 atttatgctt ctatattatt aa
//
LOCUS       DAFNNB010000180         5824 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0185, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000180 DAFNNB010000000
VERSION     DAFNNB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0185"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            182..1228
                     /gene="recA"
                     /locus_tag="L3M60_003190"
     CDS             182..1228
                     /gene="recA"
                     /locus_tag="L3M60_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBN6227822.1"
                     /translation="MSVDQEKLKALNEALGKIEKDFGKGSVMKLGEATSMSIDVISTG
                     AIGLDIAIGIGGLPRGRIVEVYGPESSGKTTVALSCVASAQKDGGIAAFIDAEHALDP
                     VYAKALGVDVDNLIISQPDTGEQALEIAEALIRSGAIDIIVIDSVAALVPKAEIDGDM
                     GDSHVGLQARLMSQALRKLTGSIKKSNCVAIFINQLREKVGIMFGNPETTTGGRALKF
                     YSSVRLDVRKIDTIKQGDKVIGSRTRVKVVKNKVAPPFKQAEFDIMYGEGISKIGDLL
                     DIAADVDIVKKSGSWYSYNDTKLGQGRENVKKFLEDNLDLTNEIDEKVRAFYNLNEEH
                     EEAGNSVSKEIVEE"
     gene            1404..2945
                     /gene="rny"
                     /locus_tag="L3M60_003191"
     CDS             1404..2945
                     /gene="rny"
                     /locus_tag="L3M60_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HBN6227823.1"
                     /translation="MDVIDSVVMIVIGAAVGIIAGYFVRKNISEAKIGQAENLAKEII
                     DKAHHDSETVQKEKLLEAKEEIHKWRTEAERENRERRTEVQKYEKRVVQKEEVLDRKL
                     QNLESKEVNLSEKLKVVEKKEEEVEVIKTQQLEKLESISGITSDKAKEIILTNAERDV
                     RREMSIMIKEIESQAKEEADKKSREIIGYAIQKCAADHVAETTVTVVSLPNDEMKGRI
                     IGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLIADGRIHPAR
                     IEEMVEKARKEVDNIIKEYGEQAAFETSVHGIHPELIRLLGRLNYRTSYGQNVLKHSI
                     EVAHIAGIMAAEIGADIRLAKRAGLLHDIGKAVDHEMEGTHVEIGMDLLRRYKESKEV
                     IHAMSTHHGDYEPQTVEAVLVTAADAISAARPGARRETLEAYIKRLEKLEEIANSYEG
                     VEKSFAIQAGREIRIMVKPEAINDEEIHLLARDMTKKIEDELEYPGQIKVSIIRETRA
                     IEYAK"
     gene            3255..5591
                     /locus_tag="L3M60_003192"
     CDS             3255..5591
                     /locus_tag="L3M60_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6227824.1"
                     /translation="MKKNTLLHTNIMVCIIITLGFVATSIIGYQSNTNIFKNDIEHVS
                     TLAAEGIYYQIDKLLSEPINVSLTMANDSLLKNFLDGEKEHLNDEEFIYKLQDYLNVY
                     RNKYLYDSVFLVSTKTNNYYHFDGLNRNLSVNNSENQWYYTFLNKEDEYSLNVDNDEA
                     SDNSITVFVNCKIKDDNGATMGIIGVGLKVNSLQMLLKGYDDKFDVVTHLIDDKGFVQ
                     LAVDKTGHEHINFFENSSSDLSNSKKLILNNEKEQKSFWYSSEKSKRYIVCQYIPSLK
                     WYLVLENDFTLMSKQLYMQFLKDIGVIVLIIIFVLFTITHVLKKYKKQIVKLSVSQKL
                     EYQKLLSQSTEGLYENIFEIDITNNRAGCEDENRYFHEFGLGLNTPYDEALHTIAKNQ
                     IKEEYAQGYLDTFLPENVIKCYNSGINNISYDFLIKKDEESYHWIRINARIFFWTSDE
                     SVRMISYRKNIDEEKNRELLLLERSQRDSLTGLYNKATTEEFIRSFLKNESNQDLEHV
                     FLIFDIDDFKNVNDKYGHVFGDFVISEFALELKSQFREDDIVGRIGGDEFVVLIKDFN
                     SNNYVLIDKLERFCSKINQKKFAEQAGFSIGCSIGVSMYPEHGYVYSELYEKADQALY
                     YAKSHGKNSYHVFSEDSTDSISFHIDPRDIKVLLEMATDGVVKFACMENLKLLYFNQK
                     MLEFTGYSAQTVTSTDFNIFSPVLQDDLQMVLDTLKEAISKRETFEVVFRFCHTDGHI
                     FPVRMQGKFVSELYQNRYPVFYAIYTDISNINTLLENK"
BASE COUNT         2345 a          617 c         1063 g         1799 t
ORIGIN      
        1 cattttaaga atattttctt ttgtgtatat aattattaat aatggtttat aaataaaatg
       61 taatgaataa aaatataatc attagtagcg tttttaaatt gactaggttt gatttatgtt
      121 atataattaa tatataacaa gagaacaaat gtttgtagat aaatgaaggg atggtataga
      181 gatgagtgta gatcaagaaa aattaaaagc gttgaatgaa gctttaggta aaattgaaaa
      241 agattttggt aaaggttcag taatgaaatt gggagaagca acatctatgt ctatagatgt
      301 tatatcaaca ggagcgattg gtttagatat agctattggt ataggaggtc tacctagagg
      361 gagaatagtt gaagtatatg gtccagaatc ttctggtaag actactgttg cacttagttg
      421 tgtagcatca gctcaaaaag atggaggaat agctgcattt atagatgcag aacatgcact
      481 tgacccagta tacgcaaaag ctttgggcgt ggatgttgat aacctaataa tatctcaacc
      541 agatacaggt gaacaggctt tagagatagc agaggcattg ataagaagtg gagcgataga
      601 tataatagta atagactcag tagcagcatt agttccaaag gctgaaatag atggagatat
      661 gggtgactct cacgtagggt tacaagctag acttatgtcg caagctctta gaaagttaac
      721 tggttcaatt aaaaaatcaa attgtgttgc tatatttata aaccagttaa gagagaaagt
      781 aggaataatg tttggaaacc cagaaactac tactggagga cgtgcattaa aattctattc
      841 atcagttaga ttggatgtta gaaaaataga cacaataaaa caaggagata aagttatagg
      901 tagtagaact agagttaaag tcgttaaaaa taaagtagca cctccattta agcaagctga
      961 atttgatata atgtatggag aagggatttc aaaaattgga gaccttctag atatagctgc
     1021 tgatgtagat atagtaaaaa aatcaggttc atggtatagt tacaatgata ctaaacttgg
     1081 acaaggaaga gaaaatgtta aaaaattctt ggaggataat ttagatttaa ctaatgaaat
     1141 agatgagaaa gttagagcat tttacaattt aaatgaagaa catgaagaag caggtaattc
     1201 agtatcaaaa gaaattgtag aagaataagt aaaaatacaa aaaaataact agtaattaaa
     1261 gacatgccac gctccctatt agcttgacac aatgttgaaa aaattataaa attaagtgag
     1321 gataaaatat ctaaatttaa ttttaattat taggaatatt ttttaatcat agtgatgaca
     1381 ttttgggatt attaacgaag gaggtggatg tcatagatag tgtagtaatg attgtaatag
     1441 gtgcagctgt tggtattata gctggatatt ttgttagaaa aaatatatct gaagcaaaaa
     1501 ttggacaagc tgaaaacttg gccaaagaaa taatagataa agcacaccac gactcagaaa
     1561 cagtacaaaa agaaaagtta ctagaagcta aagaagagat ccataagtgg agaacagaag
     1621 ctgaaagaga aaacagagaa agaagaacag aagtacaaaa gtatgaaaaa agagtagtac
     1681 aaaaagaaga agtattagat agaaaattac aaaatttaga atctaaagaa gtaaacctaa
     1741 gtgaaaaatt aaaggtagtg gaaaagaaag aagaagaagt tgaagtaata aaaactcaac
     1801 agctagaaaa attagaaagt atatcaggaa ttacttctga taaggcaaaa gaaatcatct
     1861 taaccaatgc agagagagat gtaagacgtg aaatgtctat aatgataaaa gagatagaaa
     1921 gtcaagctaa agaagaagct gataagaaat caagagaaat tataggatat gcaattcaaa
     1981 aatgtgcagc agatcacgta gccgaaacta ctgtcacagt agtaagcttg cctaatgatg
     2041 aaatgaaagg tagaataata ggtagagaag gtagaaatat aaggacttta gaaaccttaa
     2101 caggtataga ccttataata gatgacactc cagaagccgt aattttatca ggatttgatc
     2161 caataagaag agaaattgca agaattgctc tagaaaaatt aatagcagat ggaagaatac
     2221 atccagctag aattgaagaa atggttgaaa aggcgagaaa agaagttgac aatattataa
     2281 aagaatatgg tgaacaagct gcatttgaaa ctagtgtaca tggaattcat ccggaattaa
     2341 taagattatt aggtagactt aactatagaa ctagttatgg acaaaatgta cttaaacatt
     2401 ctatagaagt tgctcatata gctggtataa tggctgctga aataggagca gatattagat
     2461 tagctaagag agcaggttta ttgcatgata taggtaaggc tgtagaccat gaaatggaag
     2521 gtacacatgt tgaaataggt atggatttac ttagacgtta taaggaatct aaagaagtta
     2581 tacatgccat gagtacccat catggagatt atgaacctca aacagtagaa gctgtattag
     2641 ttacagcagc agatgcaata tcagctgcta gacctggagc tagaagagaa actttagaag
     2701 catatataaa gagacttgaa aaattagaag aaatagctaa ttcatatgaa ggtgtggaaa
     2761 aatcatttgc tatacaagct ggtagagaaa taagaataat ggtaaaacca gaagctataa
     2821 atgatgaaga aatacaccta ttggctagag acatgactaa gaaaatagag gatgaacttg
     2881 aatatcctgg acaaataaaa gtaagcataa taagagaaac aagagctata gaatatgcga
     2941 agtaaaagaa ataatatcta aataataaga aaatattttt agaaatttaa atttgccaca
     3001 agatataata tatcttgtgg tttttaaatt gtttaaattt aattttatat aatgtaaaat
     3061 aatctaagtg ctaaaagtgt tacttacaat aaattaatga aataaagatt tatatatgta
     3121 ttgtttatat aattatccaa atttgtttga tataatgaag ttgtaaaata aatatattac
     3181 tacttggaat gcttatactt aaataatatg aaaatacaat aaagctaaag tgaggtttaa
     3241 agagagggta cactatgaaa aaaaatacac ttttacatac aaatattatg gtttgcatta
     3301 ttattactct tggttttgtg gctacctcta taattggata ccaatctaat accaatattt
     3361 ttaaaaatga tattgaacat gtgtctacat tggctgctga aggtatttat tatcagatag
     3421 ataaactact ttcagaacct ataaatgtat ctcttacaat ggcaaatgat agtttattaa
     3481 aaaatttttt agatggtgag aaagaacatt taaatgatga agaatttatt tataaattac
     3541 aagattatct gaatgtatat agaaataaat acttatatga ttctgttttt ttagtatcaa
     3601 ctaaaactaa taattattat cattttgatg gacttaatag aaatcttagt gtcaataatt
     3661 cagagaatca atggtattat actttcttaa ataaagagga tgaatactca ttaaatgttg
     3721 ataatgatga agcaagtgac aatagtatta cagtatttgt aaattgtaag ataaaagatg
     3781 ataatggagc aactatggga attataggtg ttgggttaaa ggttaattca ttacaaatgt
     3841 tgcttaaagg atatgatgac aaatttgatg ttgtcactca cttaattgat gataaagggt
     3901 ttgtacaatt agcagtagat aaaactggtc atgagcatat aaattttttt gaaaattcca
     3961 gtagtgactt gtctaattca aaaaaattaa tattaaacaa tgaaaaggaa caaaaaagtt
     4021 tttggtattc atctgaaaaa tcaaaacgtt atattgtatg ccagtacata cctagtttaa
     4081 aatggtatct tgttttagaa aatgatttta ctttaatgag taaacagctt tatatgcaat
     4141 ttttaaaaga cattggagtt atagttttaa ttataatatt tgtacttttt actattacac
     4201 atgtgcttaa aaaatacaaa aaacaaatag tgaagcttag tgtttcacag aaattagaat
     4261 atcaaaagtt gttaagccaa tctactgaag gactttatga aaatattttt gaaattgata
     4321 ttaccaataa tagagctggt tgtgaagatg agaatagata ttttcatgaa tttggattag
     4381 ggcttaacac tccatatgat gaagcactgc acacaatagc taaaaaccaa atcaaagaag
     4441 aatatgcaca aggttattta gatacattct tacctgaaaa tgtgattaaa tgttacaata
     4501 gtggaattaa taatatcagt tatgactttt taattaaaaa agatgaagaa agctatcatt
     4561 ggattagaat taatgcacga attttctttt ggacatcaga tgaatctgtt cgtatgatat
     4621 cttatcgtaa aaatatagat gaagaaaaaa atagagaatt actacttctc gaacgttcac
     4681 aacgtgattc tcttacaggg ctttacaata aagctacaac agaggaattt attcgttcat
     4741 ttttaaaaaa tgaaagtaat caagacttag aacacgtttt tctaatattc gatattgatg
     4801 attttaaaaa tgtaaatgat aaatatggtc atgtatttgg agactttgtt ataagtgaat
     4861 ttgctttaga attaaaatct caatttcgtg aggacgatat tgtaggacgt attggtggag
     4921 atgagtttgt agtattaata aaagacttta atagtaataa ctatgtactt atagataaat
     4981 tagaacgttt ttgctctaag attaatcaga aaaaatttgc tgagcaagca ggattttcta
     5041 ttggatgtag tattggtgta tcaatgtatc ctgaacatgg atatgtatac tctgaactat
     5101 atgagaaagc agaccaagca ctttactatg caaaaagcca tggaaaaaat tcttatcatg
     5161 tattttcaga ggattctact gatagtattt catttcatat agaccctaga gacataaaag
     5221 tattattaga aatggctact gatggtgttg tcaaatttgc ttgtatggaa aacttaaaac
     5281 tactttattt taaccaaaaa atgcttgaat ttactggata ttcagctcaa actgttactt
     5341 ctacagattt taatattttt tcgcctgtac tacaagatga cctacaaatg gtactcgata
     5401 cattaaaaga agcaatctct aaaagggaga cttttgaggt agtttttcgt ttttgtcata
     5461 cagatggtca tatttttcct gtaaggatgc aagggaagtt tgtaagtgaa ttatatcaaa
     5521 atcgttatcc tgttttttat gctatttata cagatatatc aaatatcaat actttattag
     5581 aaaataaata gtaaatgcta cagtaaaatc taaaaatagg atataataat attaatataa
     5641 gaagattata agtaaaacct gctgataaaa tagattattt taggcaggtt tttaaatttt
     5701 ttatagtaaa tcttattttt agtcaatagg gtttattttt taagaacttt attgattaaa
     5761 aaatagctac gcacacttgt gactttagtc atgagttagt agctaaatta gtcagcatat
     5821 aggg
//
LOCUS       DAFNNB010000181         5583 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0186, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000181 DAFNNB010000000
VERSION     DAFNNB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0186"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..875
                     /locus_tag="L3M60_003193"
     CDS             <1..875
                     /locus_tag="L3M60_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_except=(pos:615..617,aa:Sec)
                     /transl_table=11
                     /product="glycine/betaine/sarcosine/D-proline family
                     reductase selenoprotein B"
                     /protein_id="HBN6227825.1"
                     /translation="KVVPIMTKLGLKLVRGEEIGPPEEEGYIMRGIRKNFFHELRGSE
                     RAIDMLVKKMKGESFETEYPMPEFDRVTPMKPVKDLSKIKLALVTSGGIVPIDNPDKI
                     ESSSATKYGIYDLTDMDSMSNKEFTTIHGGYDRAYVLENPNLVVPLDVVRELEKEGVI
                     GELVNYFITTTGTGTSVGNSKRFGEEFSKKLLEDDVDAVILTSTUGTCTRCGATMVKE
                     IERAGIAVVHICTVVPISLTIGANRIVPAVGIPYPLGNPMLGEEESKKIRKNIVLKAL
                     NALTEAIEEQTVFE"
     gene            1164..1895
                     /locus_tag="L3M60_003194"
     CDS             1164..1895
                     /locus_tag="L3M60_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227826.1"
                     /translation="MTRKKMYILISIAICDDESYMLNHLKENIELYMKQNNLSYNIEL
                     FDKAEDLLCNHEKFNIVFLDIQMEDINGMQTARELRVYGNDCFIIFVTVLNEFVFDAF
                     EVDAINYLIKPLSNEKLYKTLDKIVTKLEDNESNYITIQKGYSYWKLKLADILYCEVI
                     KRKIYVHLKKETIDYYETIENLERSLPTNFFRCHRSYIINLKYVYGYESGNAVMESGD
                     RIPISRLRQQIFSKVMLEFMKNRRR"
     gene            1897..3255
                     /locus_tag="L3M60_003195"
     CDS             1897..3255
                     /locus_tag="L3M60_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBN6227827.1"
                     /translation="MINLFASDLFLKYSFLFSFTIYRILMVIVQYYFLINFTNVNSKF
                     WCFILYSIISIFLIIISTYFNLYNLFSSQILFFLMTFLFVKYIIKQSTVLSTVSTLLV
                     YLIEQLVYGFTAPLNYLIFTSDINTDIYKFDINMALSSLITIIIAGFVYWYSSKKFNI
                     KVMNFDKYLAILIIPLLLIILFMQSFKHSSNINIDTSLGTVKLLLNTSITEEIQAFLF
                     SIIGTICVFFSLFTFKKLIQSLEDDKERAIMNQQIHAQKNYIEEAKSRLSQTISFRHD
                     FNNHLAIVNGLLKKGQILKAQDYLNKLEKVTSDLSFSCNTRNVVIDALFNNKLSIAKQ
                     LGIEVDCEVIIPKSTNMNDFDLCIVFANAIDNAIKACRVVDKQSRYLKISTKLEGGFF
                     MIEIKNSYNPINNYTSGSCIGLSNIKEIAKKYQGAISIDKTSDYFQINILLVISLHCA
                     GI"
     gene            3294..3536
                     /locus_tag="L3M60_003196"
     CDS             3294..3536
                     /locus_tag="L3M60_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227828.1"
                     /translation="MYLNKESLIYRNEEVTKMKIGNINGQAPITNGLDSCQSEVQGLE
                     QKKKALQDEIEKVRSDKNIDSETAKLKIEDLRKQIE"
     gene            complement(3992..4216)
                     /locus_tag="L3M60_003197"
     CDS             complement(3992..4216)
                     /locus_tag="L3M60_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBN6227829.1"
                     /translation="MTVYNLKLGQKGIIDNIAGNEKLMKRLLALGLIDGTEVEVKKIA
                     PLNDPIVIRFRGFDLAIRKSDAKNINLKNN"
     gene            complement(4313..5566)
                     /locus_tag="L3M60_003198"
     CDS             complement(4313..5566)
                     /locus_tag="L3M60_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:glutamate symporter"
                     /protein_id="HBN6227830.1"
                     /translation="MITLTFDIVQTLTLSIFFFLIGNLLKNKVNFLNNFCIPAPVIGG
                     LLFCFLNLFFKYFNIADISISGNLMPNFITFFFTTIGLEISINLIKKGGSVLFRYWIL
                     CGVLAFCQNILAITISKIIKLEPLLGLMCGNVSMEGGHGYSAAFGLTIENLGIEGAVG
                     VGLSAATIGLIMGGILGCPVAKFLINKYNLKPSSNIDLSVPTYNRNLNRFGSKFFRNK
                     NMVKNNNFSQTITPSVFLEQVLLIFICINTGEIISRCFYIMFNILLPSVVTCMFSAVL
                     FRNLNDKISILELNFKLIDFLKEISLGIFLTLSLMNIDLFELSTLLPPILLIVIFQVI
                     FIILFSIFICFRVLGKDFDSAIIISGLIGHGIGATPNALANMSSLTQKYGNSPKAFLV
                     VPLVSGFLLDAISIPCILFFINILT"
BASE COUNT         2214 a          640 c          807 g         1922 t
ORIGIN      
        1 gaaaggtagt tccaataatg acgaaactag gtcttaaatt ggttagaggt gaggaaatag
       61 gacctcctga agaagaagga tatataatga gaggaataag aaagaatttc ttccatgagc
      121 ttagagggtc tgaaagagca attgatatgt tagttaagaa aatgaaaggt gaaagctttg
      181 aaactgagta tccaatgcct gaatttgata gagttacacc tatgaaacct gttaaagatt
      241 tatctaagat aaaacttgca ctagtaactt ctggaggtat agttccaatt gataatccag
      301 ataaaattga atcttcaagt gctactaaat atggaattta tgatttaaca gatatggatt
      361 ctatgtcaaa taaagaattt acaactatac atggtgggta tgatagagca tatgttcttg
      421 aaaatccaaa tttagttgta ccactagatg tagttagaga acttgaaaaa gaaggtgtaa
      481 ttggagaatt agtaaattat tttataacta ctacaggaac aggaacgagt gttggtaatt
      541 ctaaaagatt tggagaagag ttttctaaga aattgttaga agatgatgta gatgctgtaa
      601 ttttaacttc tacatgaggt acttgtactc gttgtggagc aacgatggta aaagagattg
      661 agagagctgg tatagcagtt gttcatatat gtacagtagt accaatatca cttacaatag
      721 gagctaatag aattgttcca gcagtaggga taccatatcc acttggaaat cctatgttag
      781 gagaagagga aagtaaaaaa attagaaaga atatagtttt aaaagcttta aatgcactaa
      841 cagaagctat agaagagcaa acagtatttg aataataaaa atataacaca acattaaaat
      901 taaaaaaggt atcgaaaaat agttttttaa aattgtttta cgataccttt ttttgttaga
      961 taaattcgaa atattgaata ctatttgtac atttttaaat taaatttata aaaataataa
     1021 attaatttat tataatattg atacataaat ttaaattaga tatactatga aaagtgagct
     1081 tattacattt tggaacaaat attcaacata ctagaacata ttaattgaac agaaaaaatt
     1141 aactttatat aataattaga ataatgacaa gaaagaagat gtacatattg atttcaattg
     1201 ctatttgtga tgacgaatcg tatatgctaa atcatttaaa agaaaatata gaattatata
     1261 tgaagcagaa taacttatca tataatatag agctttttga taaagcagaa gatttactat
     1321 gcaatcatga aaagtttaat attgtatttt tagatataca gatggaagat attaatggaa
     1381 tgcagacagc tagagaatta agggtatatg gtaatgattg ttttataatt ttcgttacag
     1441 ttttaaatga atttgtattt gatgcttttg aagtagatgc tataaattat ttgataaaac
     1501 ctctatctaa tgagaaacta tataaaactt tggataaaat agttacaaaa cttgaggata
     1561 atgaaagtaa ttatatcaca atacaaaaag gttattcata ttggaaatta aaacttgctg
     1621 atattttata ttgtgaagtt ataaaaagaa agatttatgt tcatttaaag aaagagacta
     1681 tagattatta tgagactatt gaaaatttag agagaagttt accaacaaat ttttttaggt
     1741 gtcatcgtag ttatataatc aatttaaaat acgtatatgg atatgaaagt ggtaatgcag
     1801 ttatggaaag tggagataga atacctattt ctagactacg ccaacaaata ttttcaaaag
     1861 ttatgcttga gttcatgaaa aataggagga gataagatga ttaatttatt tgcttctgat
     1921 ttatttttaa agtacagttt tttattttct tttactatat atagaatttt gatggtaatc
     1981 gtgcaatact attttctaat caattttaca aatgttaatt caaaattttg gtgttttatt
     2041 ttatattcga taatttcaat ttttttaatc atcataagta catattttaa tttatataat
     2101 ttattttctt cacagattct attttttctt atgacttttt tatttgtaaa gtacataata
     2161 aaacaatcta ctgttttatc tacagtatca accttattag tatatttaat tgaacaatta
     2221 gtatatggat ttacagctcc attaaattac ttgatattta ctagtgatat aaatacagat
     2281 atttataaat ttgatataaa tatggcacta tcttctttaa ttaccattat aatagctgga
     2341 tttgtatatt ggtatagttc taaaaagttc aatattaaag ttatgaactt tgataaatac
     2401 ctagcaattt tgataattcc attgttgctt ataatattat ttatgcaatc atttaaacat
     2461 agttcaaata taaatataga tacaagtctt ggtactgtaa agttgctatt aaatacttct
     2521 ataactgaag aaattcaagc ttttcttttt tctataattg gaactatatg tgtatttttt
     2581 tctttattta cctttaagaa actaattcaa tctctcgaag atgacaagga gagggcaatt
     2641 atgaatcaac agattcatgc tcaaaaaaac tatattgaag aagcgaaatc acgattatca
     2701 caaactatat cctttaggca tgattttaat aatcatttag ctattgtcaa tggattattg
     2761 aaaaaaggtc aaattttaaa agcacaagat tatttaaata agcttgaaaa agtaacaagt
     2821 gacctatcat tttcttgtaa tacacggaat gttgttattg atgctctttt taacaataaa
     2881 ctaagtatag caaaacaatt aggcatagag gtggattgtg aagtaattat acccaaaagc
     2941 actaatatga acgattttga cctatgtata gtatttgcta atgctattga caatgcaatt
     3001 aaagcttgca gagttgtaga taaacaaagt aggtatttaa agatttctac taaattagag
     3061 ggtggatttt ttatgataga aataaaaaat agctataatc caataaataa ttatacaagt
     3121 ggttcatgta tagggctttc aaatataaaa gaaattgcta agaaatatca aggggctata
     3181 agtatagata agacatcaga ttattttcaa ataaatatat tgttagttat ttcattacat
     3241 tgtgcaggta tttgagaaca atttcattga aaatagtgtt tataagctat attatgtatt
     3301 tgaataaaga aagtttgata tatagaaatg aggaggttac taaaatgaaa attggaaata
     3361 taaatggtca agcaccaatt acaaatggct tagatagttg tcaaagtgag gtacaaggct
     3421 tagaacaaaa gaaaaaagca ttacaggatg aaatagagaa agtaagaagt gataaaaata
     3481 ttgatagtga aacggccaaa ttaaaaatag aagatttaag aaaacagata gaatagattg
     3541 aaagtaaaat tcaagagtta aaaggagata aagcacaaga aacaaataaa gaagaaaata
     3601 acgtttcaga agtaaataat aatctattag acagaaaaaa agaaccttct aataatgaaa
     3661 atgtaaaaca attatatgat gtttttgaaa gaaacaatgc agacgaacaa tgggataata
     3721 cttatcatgt tgttcaagat gataaaaact taaaagtaaa atttaatagg cctatatagt
     3781 ttatttataa aaatagttga tattttaatt atcaacattt tttagtagaa tacacaattt
     3841 atttacagtt agtacattta taggtggttt tataactatt atttacaagt ctatcttgat
     3901 aacttttaca tggtatataa tgcttaaata tttattcaca tttatctata cataaacaaa
     3961 aaaagagatt ctaaaatctc ttttttattt attaattatt ttttaaattt atattttttg
     4021 catcagattt tctaatagct aagtcaaacc ctctaaatct aatcactata gggtcattaa
     4081 gaggagctat ctttttcact tcaacttcag tgccatctat taatcctaag gctagaagtc
     4141 tcttcataag tttttcattt ccagctatat tgtctataat tcccttttgt ccaagtttca
     4201 agttgtaaac agtcattatt aacacctcct aatgaaaatc attatcaaac tttatattta
     4261 taatatataa caaaaagagc aaattgtcaa tatataacaa aaaataaaca tactaagtta
     4321 gtatgtttat aaaaaaaagt atgcatggga tactaatagc atctagcaaa aaaccactta
     4381 ccaatggtac aaccaaaaat gcttttggcg aatttccata tttttgtgtc aaagaactca
     4441 tatttgccaa agcgtttgga gtagctccta ttccatgtcc aatcaaacca cttattatga
     4501 tggcagaatc aaaatctttt ccaagtactc taaagcaaat aaatattgaa aataggatta
     4561 taaagattac ctggaaaatt actattaaga gaattggagg taataatgta gatagttcaa
     4621 acaagtctat attcataagt gataaagtta aaaaaattcc taatgaaatt tcttttaaaa
     4681 agtctataag cttaaaattt aattctagta tacttatctt gtcatttaaa tttctaaaaa
     4741 gaacagccga aaacatacaa gttacaacag aaggtaatag gatattaaac attatataga
     4801 agcatctact tataatttct cctgtattta tgcaaataaa tatgagaaga acctgttcca
     4861 aaaacacaga tggtgttatt gtttgagaga agttattatt ttttaccata ttcttatttc
     4921 taaaaaattt acttccaaat ctatttagat ttctattata tgtaggaacg gataagtcta
     4981 tatttgatga tggttttaaa ttatatttat ttattagaaa tttagctaca ggacatccca
     5041 atataccacc cataataagt cctattgttg cagcagataa gccaactcca actgctcctt
     5101 ctataccaag attttctatt gtaagtccaa atgcagcaga ataaccatga ccaccttcca
     5161 tagatacatt tccacacata agccctaaaa gaggttcaag ttttataatc tttgatattg
     5221 taatagctaa gatattttga caaaatgcca atactccaca caaaatccaa tatctaaata
     5281 atacactacc acctttcttt ataagattta tacttatttc aagtcctata gttgtaaaaa
     5341 agaaggttat aaaatttggc attaaatttc cacttataga tatatctgct atattaaaat
     5401 atttaaaaaa taaatttaga aaacaaaata atagacctcc tattactggt gcaggtatac
     5461 agaaattgtt taaaaagtta actttattct tcaataaatt accaattaaa aagaagaata
     5521 tagatagtgt caaggtttgc actatatcaa aagttaaggt aatcaaaata agtcctcctt
     5581 tgt
//
LOCUS       DAFNNB010000182         5450 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0187, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000182 DAFNNB010000000
VERSION     DAFNNB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0187"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..737
                     /locus_tag="L3M60_003199"
     CDS             <1..737
                     /locus_tag="L3M60_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBN6227831.1"
                     /translation="SIYKNFHSGKSFNMKVSKKEIEKLFAKLVYDAFSEKMNKSIGFV
                     VDFKKPCETILSYSFNKQDISDEVLGNFMLDEFARLNEIVNKDIDFNEQIEFEREPTF
                     DDKFCKDLGNHFRSRLNTEFPWNLFFRILFEIEPYNNTKDFENFVEFIKENVLLEENR
                     EQLLCQFEEDSKLIIEDIYDCIFNSISEIDSVEILYNYNLEKINFTSSEIITFICREH
                     ECYGEYLEEKEKLMNTFKEKIIQIFE"
     gene            762..1370
                     /locus_tag="L3M60_003200"
     CDS             762..1370
                     /locus_tag="L3M60_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HBN6227832.1"
                     /translation="MRHFSNIYELSKIILEEAICIEKTGVILAGGKNSRMGRDKAFLE
                     LHEKLFIEIAIEAFKNFDELIIISNNEELYSKYDIRVYNDIVKDVGPIGGIYTALEYA
                     KYDIVTIACDMPYLNSEVVERIANKMKDKSVISVTNGKLQPLCSGYKKSIINKVSLCI
                     RENDLKLRSFIDKIDKSYIYFDEEDLFLNVNTVDEYKKLTKV"
     gene            1418..1789
                     /locus_tag="L3M60_003201"
     CDS             1418..1789
                     /locus_tag="L3M60_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBN6227833.1"
                     /translation="MNLLKCDSWAVILNNSNKESKAYKILDELKRNMYKVVAIDEEKK
                     PIEGIDVYECLKDVPHNIDVVAIIDKQSKMDVILEEVELLDIQNMWFEKGSFSEMMIK
                     KTKDLKLNIEYNLSLYDELTR"
     gene            1947..2285
                     /locus_tag="L3M60_003202"
     CDS             1947..2285
                     /locus_tag="L3M60_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBN6227834.1"
                     /translation="MLIVTTEKVEGKKISKVLGLVRGSTIRAKHVGKDIGASFKNLVG
                     GELTGYNEMLTEARQIAIGRMVEDAEAKGANAVIAFRLSSASVMQGAAEMLAYGTAVV
                     LEDDNSILEK"
     gene            2752..3255
                     /locus_tag="L3M60_003203"
     CDS             2752..3255
                     /locus_tag="L3M60_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HBN6227835.1"
                     /translation="MFNVAIITLSDKGYEGKREDITGKKLTEFVENTGAYKVTEYVLI
                     KDDKEMLKENIIRLCNSNRIDLILTNGGTGFSKRDITPEATKEVLEKEIPGLSEYMRM
                     KSTEITKKAILSRGVSGIRNNSIIINLPGSPKGAVENLSFIIDVLDHGIEILRGQVTE
                     CATKKSK"
     gene            3302..4261
                     /gene="moaA"
                     /locus_tag="L3M60_003204"
     CDS             3302..4261
                     /gene="moaA"
                     /locus_tag="L3M60_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBN6227836.1"
                     /translation="MIDKYGRKIDYLRISLTDKCNLRCVYCMPPDVKFDKNYINENLS
                     FEDYKFIIKAMAEQGITKVRFTGGEPLLYPKLNELIKFTKEECGINNIGMTTNAIGLS
                     DRILELEKCGLNKVNISLDSLKEYKYKSITRGGNLKDVLKSIESCLNLGIKVKLNCVA
                     ISGFNDDELKDFMFLTVKSPIDVRFIELMPLGEANRVYKKGYFNVKEMIEKIYGLYKV
                     KNDEKSTAEYYMFKGAKGRIGIITPLSCSFCNTCNRIRLTSSGAIKLCLHSKEEIDIK
                     PFLHKPLLFRESMKDIIKQKPEKHHLIENKCSDTNRRMYEIGG"
     gene            4265..4735
                     /gene="moaC"
                     /locus_tag="L3M60_003205"
     CDS             4265..4735
                     /gene="moaC"
                     /locus_tag="L3M60_003205"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBN6227837.1"
                     /translation="MLTHINEKGYAKMVDISEKDDTKRTAVATATITLSKEALEKVKN
                     GQIKKGDVLSVAQVAGIMGAKNTSSNIPMCHQIKLVGCDLEFDIDDSMSAIKIYATCK
                     SLGKTGVEMEALNACSLAALTIYDMCKAVDKKMVISNIHLVEKTGGKSGDFKFE"
     gene            4767..5198
                     /locus_tag="L3M60_003206"
     CDS             4767..5198
                     /locus_tag="L3M60_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HBN6227838.1"
                     /translation="MAKVVSINISEKKGVIKVPVQCAELKINHGIVGDAHAGNWHRQI
                     SLLGKESIDKMKMKGFDQLKYGDFAENITTEGIEVFSLPIGTKLRIGQCEVEVTQIGK
                     KCHNGCEIKKLTGDCVMPREGIFVQVLKEGIINVNDSIDVI"
BASE COUNT         2265 a          432 c         1009 g         1744 t
ORIGIN      
        1 taagtattta taaaaacttt cattcaggga agtcttttaa tatgaaagtg agtaaaaaag
       61 aaattgaaaa attatttgcg aaactagtat atgacgcttt ttctgaaaag atgaataaaa
      121 gtataggttt tgttgtagat tttaaaaaac catgtgagac tatattgagc tattctttta
      181 ataagcaaga tataagtgat gaagttttag gtaattttat gcttgatgag tttgcaagat
      241 taaatgaaat tgtaaacaag gatatagatt ttaatgagca aatagaattt gaaagagaac
      301 cgacatttga tgataagttt tgtaaagacc taggaaatca ttttagaagt agattaaaca
      361 cagaatttcc atggaacctg ttttttagaa tattatttga aatagagcca tataacaata
      421 ccaaagactt tgaaaatttt gtagagttta taaaagaaaa tgtgctacta gaagaaaata
      481 gggaacaatt attatgccaa tttgaagaag atagcaaact tataattgaa gatatatatg
      541 attgcatatt taatagtata agtgagattg atagtgttga aattttatat aattataatc
      601 ttgaaaaaat aaactttaca tcaagtgaaa ttataacttt tatatgtaga gaacatgagt
      661 gttatggtga atacttagaa gaaaaagaaa agcttatgaa tacatttaaa gaaaagatta
      721 ttcaaatctt tgaataataa gtcaatgctg aagtggcgct aatgcgccat ttttcaaata
      781 tatatgaatt aagtaaaatt attttggagg aggcgatttg tatagagaaa acaggtgtaa
      841 ttttggcagg gggaaaaaat tctagaatgg gtagagataa agcctttcta gagttacatg
      901 aaaagctttt tattgaaatt gcaatagaag catttaaaaa ctttgatgag ttgataataa
      961 tatctaataa tgaagaatta tattctaaat acgatattag agtttataat gatatagtaa
     1021 aagatgttgg tccaattgga ggaatatata ctgctcttga atatgcaaag tatgatatag
     1081 ttacaattgc atgtgatatg ccatatctaa atagtgaagt agtcgaaaga atagctaata
     1141 aaatgaagga taaaagtgtg atttcagtga caaatggaaa gttacaacca ttgtgttctg
     1201 gatataaaaa gagtataata aataaagtat ctttatgtat aagggaaaat gatttaaagc
     1261 tcagaagctt tatagataaa atagataaaa gctatatata ctttgatgaa gaagatttat
     1321 ttttaaatgt aaacactgtt gatgagtaca aaaaattaac taaagtttaa ttagctatgg
     1381 ttgtaaaata gagtaggatt tatatggagg agagtttatg aatttactta aatgcgattc
     1441 ttgggcagta atattaaata atagcaataa agaaagtaaa gcatataaaa tattagatga
     1501 acttaaaagg aatatgtaca aagttgttgc tatagatgaa gagaagaaac ctattgaagg
     1561 tatagatgtg tatgaatgtt taaaagacgt tccacacaat attgatgttg tagctattat
     1621 tgataaacaa tctaagatgg atgtaatttt agaagaagta gagcttttgg atatacaaaa
     1681 tatgtggttt gaaaaaggaa gctttagtga aatgatgatt aaaaagacta aagatttaaa
     1741 attaaacata gaatataatt taagtttata tgatgagttg actagataaa attatgtact
     1801 ttaataataa aaatataatt agttagcaaa acatattatt tcatagtata gattggttaa
     1861 tgaaacatta aattacaata tttgttaaaa aattatgagt tacatgatat aatatattaa
     1921 ataggatact aaggagttgg tatattatgt taatagtaac tacagaaaaa gtagaaggca
     1981 aaaagatatc aaaagtttta ggcttagtga gaggaagtac aataagagca aaacatgttg
     2041 gaaaggatat aggagcaagt tttaaaaatc ttgttggagg agaacttact ggatataatg
     2101 aaatgcttac tgaagcaaga caaattgcca taggtagaat ggttgaagat gcagaagcta
     2161 aaggtgcaaa cgcagtaata gcatttagat tgtcttcagc atcagttatg caaggggcag
     2221 cagaaatgct tgcttatgga acagcagttg ttttagaaga tgataatagt attcttgaaa
     2281 aataaaatat aaatagttaa aaagaggcat cttttaaaaa ttttatggat gccttttttt
     2341 atttgaaaaa actaatttca gaagaaattt taattattat gacaataaaa ttagggtgat
     2401 attatataaa taagtaaaat tatattatat aatgtatggt atagacttta aaaattttat
     2461 tggtagagaa aaatatggac aatgttataa taattataga aattaaaagt acttacattc
     2521 attatatatg ttacatgtat aattagtttt atatttaatg cagttttgaa gtactatctt
     2581 cattttgaga acgttggaat aataaaaaat gtatgtatta taagtgatat aaataaaaaa
     2641 taaatataat atgttgtatt gctgatagaa aagggaataa agtaaagtag gttttattga
     2701 aagagatttt tatttatagg acttaatcaa tgtatagagg ggggagagca tatgtttaat
     2761 gttgcaatta taacacttag tgataaagga tatgaaggaa aaagagaaga tataacagga
     2821 aaaaaattaa ctgaatttgt tgaaaataca ggagcttata aagttactga atatgtactt
     2881 ataaaagatg ataaggaaat gttaaaggaa aatattataa gactttgtaa tagtaataga
     2941 atagatttaa ttcttacaaa tggaggtact ggtttttcaa aaagagatat aactccagaa
     3001 gcaactaagg aagtattgga aaaagaaata ccaggtctga gcgaatatat gagaatgaaa
     3061 tctacagaaa ttactaaaaa ggctatacta agtaggggtg tgagtggaat aagaaataat
     3121 tctataataa taaatttacc tggaagtcct aaaggtgcag ttgaaaattt aagttttata
     3181 attgatgttt tagatcatgg catagaaatt ttaagaggac aagttacaga atgtgccact
     3241 aaaaaaagta aatagtatat aatctagata gataaaaaat agacaaagag gtagatagag
     3301 tatgattgat aagtatggaa gaaaaataga ctaccttcgt atttcactta cagataagtg
     3361 taatttaagg tgtgtctatt gtatgccacc agatgtaaaa tttgataaaa attatataaa
     3421 tgaaaatcta agttttgaag attacaagtt cattataaag gctatggctg aacaaggtat
     3481 aacaaaagtt agatttactg gaggagaacc tcttttatat cctaaattaa atgagcttat
     3541 aaaatttaca aaagaagaat gtggaataaa taatataggc atgaccacta atgctattgg
     3601 gctttctgat agaatacttg agcttgaaaa atgtggctta aataaagtta atataagttt
     3661 agactctttg aaggaatata agtataaatc cataacaaga ggaggaaact taaaagatgt
     3721 tttaaaatca attgaatctt gtttaaattt aggtataaag gtaaagttaa attgcgttgc
     3781 tataagtggg tttaatgatg atgagcttaa agattttatg tttttaacag ttaaatcacc
     3841 aattgatgta agatttatag aacttatgcc attaggtgag gctaatagag tttataagaa
     3901 ggggtatttt aatgtaaaag aaatgattga aaaaatatat ggtttatata aagtcaaaaa
     3961 tgatgaaaag agtacagcag aatattatat gtttaaagga gctaaaggaa ggataggaat
     4021 tataactcca ttaagctgtt cattttgtaa cacttgtaat cgtattagac ttacttctag
     4081 tggtgcaata aaattatgtc tacattcaaa agaagaaata gatataaaac catttttaca
     4141 taaaccattg ttatttagag aatctatgaa agatataata aaacaaaaac cagaaaaaca
     4201 tcatttaata gaaaataaat gtagtgatac aaataggcgt atgtatgaaa ttggaggata
     4261 agtaatgtta acacatataa atgaaaaagg gtatgccaag atggtggaca taagtgaaaa
     4321 agatgatact aaaagaacag ctgtggctac agctacaatt actcttagta aggaagcttt
     4381 agaaaaagta aaaaatggtc agataaaaaa aggagatgta ttgtctgtgg ctcaagttgc
     4441 aggtataatg ggagctaaaa atacatcttc aaacatacca atgtgccatc agattaaatt
     4501 agttggatgt gatttagaat ttgatataga tgattctatg tctgctatta agatatatgc
     4561 aacctgtaaa tctcttggga aaactggagt agaaatggaa gctttaaatg catgttcttt
     4621 agcagcactt acaatttatg atatgtgtaa agcagttgat aaaaaaatgg ttataagtaa
     4681 tatccatctt gtggagaaaa ctggtggaaa atcaggagat tttaagttcg agtaaattaa
     4741 taatatattg aaaatttgga ggaaatatgg ctaaggtagt gtcaataaat ataagtgaga
     4801 aaaagggtgt aattaaagtt cctgtacaat gtgcagagct aaaaataaat catgggatag
     4861 ttggagatgc acatgcagga aactggcatc gtcagataag cttacttgga aaagaaagca
     4921 tagataaaat gaaaatgaaa ggctttgacc aattaaaata tggagatttt gctgaaaata
     4981 taactacaga aggtatagaa gttttttccc tgcctattgg tacaaagcta agaatagggc
     5041 aatgtgaagt tgaagtaact caaataggaa aaaaatgtca caatggatgc gaaattaaga
     5101 aattaactgg ggattgtgta atgccacgag aaggaatttt tgtacaggtt ctgaaagaag
     5161 gaattataaa tgtgaatgat tcaatagatg taatttaatg aagttagtat taaaatataa
     5221 ttataagtaa atattattaa gatttaaatg tatattttaa gagagaatta attttaataa
     5281 aatattttag ttataaattg aaatattggt tgtataaagc tatgaatgta aaatcatagc
     5341 tttttgtttt gtgattttaa aatattatat tttatttata tatattgaat tatattatat
     5401 aaaattaatt gtttaaatgt atgttattta tactgtaaat ttatagataa
//
LOCUS       DAFNNB010000183         5346 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0188, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000183 DAFNNB010000000
VERSION     DAFNNB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0188"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            252..428
                     /locus_tag="L3M60_003207"
     CDS             252..428
                     /locus_tag="L3M60_003207"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227839.1"
                     /translation="MNLNRIKEIIKKGLKKFMKVVVNLFCNKLFHRMLYVISISVVVK
                     ITMNINLYISILKY"
     gene            529..1266
                     /gene="cas6"
                     /locus_tag="L3M60_003208"
     CDS             529..1266
                     /gene="cas6"
                     /locus_tag="L3M60_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endoribonuclease Cas6"
                     /protein_id="HBN6227840.1"
                     /translation="MSRISVFMESCNGKFPNENSMLSVSFIKNILNAENKDFAKNIFN
                     YGEERKNNKQIKDINTAIYIPNLIRSKNELLVDGDIIFNISFYNYSMFSKLYNGILKV
                     KELEYKGYRFKIKNIKIHKEHEIKENGVIFKTMSPIIVKNKEGKYLDVEDSNYIECLN
                     YIANLTLESIRGSGLRKPLEFIPLNFKKRVLKEKIRGFKEREFYYINAYAGTFFLKGD
                     MEDLNALYKMGIGYRRTENAGMVDILK"
     gene            1271..2737
                     /locus_tag="L3M60_003209"
     CDS             1271..2737
                     /locus_tag="L3M60_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227841.1"
                     /translation="MREIKAKFYRSGDIVRDIGIVYFYELLMDLKNELEKSDYKLKFT
                     CELNRNYLSLESAENIDPKYISDYILENQLFKVFKNGKKEALEKIFNNIDNLTYVNFI
                     EELDKSSATAKQKEDIKKDFKNRRFPYVRNSGKYGLNTSLENMEINVKNIVDTVIKSN
                     IEIDKIDLEQYEEKDSVCNVCNINKTTKLDIDLRERKDSKYNFLFRGAEKSGFKRSGQ
                     VESNICFECEFFNLMCLLYINLKRPMVFAYTNDLRELAFLNHKIMLKRKMYSDKSFYK
                     KLLHEKISSIRLYRFDIDTNKGIILKFDSIIEYKELLKKIELIDIIDNYNFSREPGNT
                     KNLGKKMIDNGNLSNLKELLLDNISFLKPSNGTSREMDFVSSSYNIGLYIKLCWIVDG
                     GDEYKMKNYTESNLIYSRVGKDLFNKMTDESRRKNFSMKVIQLLKSNDRTQLFQTIMH
                     VLVSNGILIGDGFVEGLMQSSELELNTNVGLFIQEIMK"
     gene            2756..3769
                     /locus_tag="L3M60_003210"
     CDS             2756..3769
                     /locus_tag="L3M60_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DevR family CRISPR-associated autoregulator"
                     /protein_id="HBN6227842.1"
                     /translation="MNEKRVKGVTLTILTESPVPLSYDQGYGNYTPIKKEQYREKIHA
                     KTSIATITYDLRRMLHQEYGWNLSNIIFGKKGNIYPSIKKNVGCVDENGLETDVFGYL
                     IPLKDKGSISKASPLRIIPFRSLNPYIGSTQLITNRGFLSSEFGRKYYDEKEENEVLR
                     DENFPTTQALAIEETLSDYYVYTITLELDRIGVVEVEDGKLLLPEERKFMSKELREKA
                     VKDILDAITVFTRNIKHSSVLLKPLAVMGGAFDKVVPFFWDDVDYNADSGEINLEGVI
                     ETIESYSLKESNTILAINDRLKISNKKELNKFNLSKYPVKEIKNLADRLEIGEDNMWY
                     LKE"
     gene            3785..4582
                     /gene="cas5"
                     /locus_tag="L3M60_003211"
     CDS             3785..4582
                     /gene="cas5"
                     /locus_tag="L3M60_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HBN6227843.1"
                     /translation="MKVLQCKLKQPTAHYRDPKVFQNEYISTLNLPSKTTIMGMITYL
                     CDRRLNSDIDIGIIGTHHHRELEFSRGENIDFWNEYTNMRKGKDKEKFLLKGNYYDYY
                     KEHKAQNSILNYEVLKDVELTIFISCKDDEELEFIRKKLESPCKYANLGRKEDFVIPS
                     EKGCFVKEVELKEVIPMNTRDAIKENIKLKNTYVRVDLRDKENVETIINQGVLIALPH
                     KYKDLEANRDDRVYEFCHYIYVDNDGIYPKNIKVNVCIDTKEVFTWL"
     gene            4609..>5346
                     /locus_tag="L3M60_003212"
     CDS             4609..>5346
                     /locus_tag="L3M60_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HBN6227844.1"
                     /translation="MENIMQKEILAKSYNLRTNQQEQTLVEHIKDLFEVLESILELNL
                     YSDKDVEILKICCALHDLGKINSIMQQKMEINNKISYSCSEEERKKLESDKKSLKKIA
                     RHNIFSGAFLKDILEKMNLSEEDKLYIYKSIMLHHGNYEDYMRLSTSKVQEEIYEYIE
                     KGILEKEEFNLKDIESYINDILNVDFKFGEDVLDYDYIDKLSESMFIDSDYNNGQIED
                     SVLNYRKFKYILYKGMLNLIDHSASSRQ"
BASE COUNT         2329 a          428 c          898 g         1691 t
ORIGIN      
        1 ttaaatgtca atttaattta taaggaaata gtattatatt aatagtttag attaacaatg
       61 aggagtataa aaataatata attaagattg tattgattgt atataattaa atttctagta
      121 aatataatta tatacaatta tatttatccg gtcaaattaa ttataaagaa ataattattg
      181 actctatata agtaatatca tataatgtaa atatgattta ataaatataa aaatacatag
      241 ggggaataaa tatgaattta aacagaataa aagaaataat taagaaagga ttaaaaaaat
      301 ttatgaaagt agtagtaaat ttattttgta ataaattatt tcatcgcatg ttatacgtta
      361 tttctatttc agtagtagta aaaatcacaa tgaacataaa tttatacata tctattttaa
      421 aatattaact attttgtaaa tataaaaaat taatagatat atatctatta attttttata
      481 ttacaaatag agaataatgg attatactag aaagtaggag gtgaaaatat gagtagaatt
      541 tcagttttta tggagtcatg taatggcaaa tttccaaatg aaaattcaat gttgtcagtt
      601 tcctttataa aaaatatttt aaacgcagaa aacaaagatt ttgctaaaaa tatatttaac
      661 tatggggaag aaagaaaaaa caataagcaa ataaaggata taaatacagc catctacata
      721 ccaaatctta taagaagcaa aaatgagttg cttgttgatg gagatataat attcaatata
      781 agtttttata attattctat gttcagtaaa ttatacaatg gaattttaaa agtaaaggag
      841 ctagaatata aaggttatag atttaaaata aaaaatataa aaatacataa agaacatgag
      901 ataaaagaaa atggtgttat atttaaaact atgtctccta taatagtaaa gaataaagaa
      961 ggtaaatatt tagatgtaga agattcaaat tatatagaat gtttaaacta tatagctaat
     1021 ctaactctgg agagtataag aggtagtggg cttagaaagc cattggaatt tatacctctt
     1081 aacttcaaaa aaagagtgtt aaaagaaaag attcgtggat ttaaagagag agaattttac
     1141 tatataaatg cttatgcagg aacatttttt ttaaaaggag atatggaaga tttaaatgca
     1201 ctgtataaaa tggggattgg atatagaagg acggaaaatg caggaatggt tgatatatta
     1261 aagtagcgag gtgagagaaa taaaagctaa attttataga tcaggtgata ttgtaagaga
     1321 tattggcatt gtttactttt atgaattact aatggattta aaaaacgaac tagaaaaaag
     1381 tgattataaa ttaaaattta cttgtgagtt aaatagaaat tatttatctt tagaatctgc
     1441 agaaaatata gaccctaagt atatatctga ttatatatta gaaaatcaac tttttaaggt
     1501 atttaaaaat gggaaaaagg aagccttaga aaaaatattt aataatatag ataatttaac
     1561 gtatgtaaat tttattgaag aattggataa atcttctgca actgctaaac aaaaggaaga
     1621 tattaaaaaa gattttaaaa acagaagatt tccatatgtt agaaatagtg gtaagtatgg
     1681 attaaacacg agtttagaaa atatggaaat aaatgtaaaa aacatagttg atactgtgat
     1741 taaatcgaat atagaaatag ataaaattga tttagaacaa tatgaagaaa aagatagtgt
     1801 atgtaatgtt tgtaatataa ataaaacaac aaaactagat attgatttaa gagagagaaa
     1861 ggactctaag tataactttt tatttagagg tgcagaaaaa tcaggcttta aaagaagtgg
     1921 acaagttgaa agtaatattt gttttgagtg tgaatttttc aacttaatgt gtcttttata
     1981 tataaattta aaaagaccaa tggtttttgc atatacgaat gatttaaggg aattagcatt
     2041 tttaaatcat aagataatgc ttaaaagaaa aatgtattca gataaatcat tttataaaaa
     2101 acttttacat gaaaaaataa gttcaattag actatatagg tttgatattg atacgaataa
     2161 aggtataatt ctaaagtttg attctattat agagtataaa gaattactaa aaaaaattga
     2221 acttatagat attatagata attacaattt ctcaagagag ccagggaaca ctaaaaattt
     2281 aggtaaaaaa atgattgata atgggaattt atcaaattta aaagaattat tacttgacaa
     2341 tataagtttt ttaaagccat caaatggaac ttctcgagaa atggattttg tatctagttc
     2401 atataatatc ggcttatata taaaattgtg ttggattgta gatggagggg atgagtataa
     2461 aatgaaaaac tatacagaaa gtaatttaat atattcaaga gtaggtaaag acttatttaa
     2521 taaaatgaca gatgaatcca gaagaaaaaa tttttctatg aaagtgattc aactacttaa
     2581 atctaatgat agaactcagt tatttcaaac cataatgcat gtgttagttt ctaatggaat
     2641 cttaatagga gatgggtttg tagaaggtct aatgcagagt tctgaattag aattaaatac
     2701 aaatgtaggt ctatttattc aagaaataat gaaataaaaa gggggaaaat ttaaaatgaa
     2761 tgaaaaaaga gtaaaaggag taacattaac tatactgaca gaaagcccag ttccactttc
     2821 atatgaccaa ggttatggaa attatacacc aataaaaaaa gaacaatata gagaaaaaat
     2881 acatgcaaaa actagtatag ctacaataac atatgactta agaagaatgc tacatcaaga
     2941 atatggttgg aatttaagta atattatttt tgggaaaaaa ggaaatatat atccatctat
     3001 taagaaaaat gtaggatgtg tagatgaaaa tggattagag actgatgtat ttggttattt
     3061 gataccactt aaagataaag gttctataag taaagcttct ccacttagaa taataccttt
     3121 taggtcatta aatccatata taggttcaac acagctaata acaaatagag gttttttaag
     3181 tagcgaattt ggtagaaaat attatgatga gaaggaagaa aatgaagtac ttagagatga
     3241 aaactttcca acaacacaag cattggcaat agaagagact ttatcagatt actacgttta
     3301 tacaataaca ttggagcttg atagaatagg agtagtagaa gtagaagatg ggaagttact
     3361 tttaccagaa gaaagaaaat ttatgagtaa ggaattaaga gaaaaggcag taaaagatat
     3421 attagatgca attactgtgt ttacaagaaa cataaaacat tcatcagtac tattaaaacc
     3481 acttgcagta atgggtggag cttttgataa agttgtaccg tttttttggg atgatgtaga
     3541 ttacaatgct gatagtggtg agataaattt agagggagtt atagaaacta tagaaagtta
     3601 tagccttaaa gaaagtaata caatacttgc tattaatgac agattaaaaa tttctaataa
     3661 aaaggaatta aataagttta atttaagtaa ataccctgta aaagagatta aaaatctagc
     3721 agacagattg gaaattggag aagataatat gtggtattta aaagagtagg aggttttttg
     3781 aattatgaaa gtattacaat gtaaacttaa gcaaccaact gctcactata gagacccaaa
     3841 agtatttcaa aatgaatata taagtacttt aaatttacca tctaagacta caataatggg
     3901 gatgataacg tatttatgtg atagaagatt aaactctgat atagatatag gaataattgg
     3961 aacacatcat catagagaac tagaattttc aagaggagag aatattgatt tttggaatga
     4021 atatacaaat atgagaaaag gtaaagataa agaaaagttt ttgcttaaag gaaactacta
     4081 tgattactat aaagaacata aagctcaaaa tagcatatta aactatgaag ttcttaaaga
     4141 cgtagaactg actatattta tttcttgcaa ggatgatgaa gaacttgaat ttattagaaa
     4201 aaagcttgaa agtccatgta aatatgcaaa tttaggaaga aaagaagatt ttgtaatacc
     4261 aagtgaaaaa gggtgttttg tgaaagaagt tgagttaaaa gaggttatac ctatgaatac
     4321 tcgtgatgct ataaaagaaa atataaaatt aaagaataca tatgtaagag ttgacttaag
     4381 agataaagag aatgtcgaga ctataataaa tcaaggtgta ttgattgcat tgcctcataa
     4441 atataaagat ttagaagcaa atagagacga tagagtttat gagttttgcc attatatata
     4501 tgttgataat gatggtatat atcctaaaaa tattaaggta aatgtatgta ttgatacaaa
     4561 agaagttttt acatggcttt aaattatttt gaaagatagg tgtttatcat ggaaaatatc
     4621 atgcaaaaag aaatattagc taagtcatac aatttaagaa ctaatcaaca agagcaaaca
     4681 ttagttgaac atattaagga tttatttgaa gtattagaat ctatattaga actaaatcta
     4741 tattcagata aagatgtaga gatattgaaa atatgttgtg ctttgcacga tttaggaaaa
     4801 attaattcga ttatgcaaca aaaaatggag attaataata aaatatcgta tagttgttca
     4861 gaagaagaga ggaaaaagtt agagtctgac aaaaaatctt taaaaaagat agcaagacac
     4921 aatatatttt ctggagcatt tttaaaagat atattagaaa aaatgaactt gtcagaagaa
     4981 gataagttat atatatataa aagtataatg ttacatcatg gaaactatga agattatatg
     5041 aggttaagta ctagtaaagt tcaagaagaa atttatgagt atattgaaaa aggcatttta
     5101 gaaaaagaag agtttaatct gaaagatata gaaagttata taaatgacat tttaaatgta
     5161 gattttaaat ttggtgaaga tgtattggat tatgattaca tagataaatt aagtgaaagt
     5221 atgtttatag acagtgatta taacaatggt caaatagaag attcagtttt aaattataga
     5281 aagtttaagt atattttata taaagggatg ttgaatttaa tagaccatag tgcatcaagt
     5341 agacag
//
LOCUS       DAFNNB010000184         5344 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0189, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000184 DAFNNB010000000
VERSION     DAFNNB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0189"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            491..2119
                     /gene="aspD"
                     /locus_tag="L3M60_003213"
     CDS             491..2119
                     /gene="aspD"
                     /locus_tag="L3M60_003213"
                     /EC_number="4.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 4-decarboxylase"
                     /protein_id="HBN6227845.1"
                     /translation="MKNHTYKEIKNIYGKISPFEFKDKLIDIAKYTAKENNRELLDAG
                     RGNPNWTCSTAREAFFTFGHFAINETRSNWDLGHLAGMPQKKGIKERFFKFINENIDM
                     PGAYLARDIINFGINELGFDGDEFIHELADGIIGDNYPLPDRMLPHMEKIVHDYLVQE
                     MKYDISGKYGDVEIFAVEGATAAMCYIFDSLMANELLKKGDTIALMTPIFTPYLEIPN
                     LPRYDFKVVNINANEVDEKGAHTWQYTKKELEKLRDKSIKALFVVNPNNPASIAMDET
                     SCNNLIDVIENYNKDLMIISDDVYGTFVEDFSSLMSKLPYNTVGVYSYSKYFGVTGWR
                     LGTFALHKKNVFDKKINDLTGELKKSVDKRYSDMSLNPSSLSFMERVVADSRLVALNH
                     TAGLSTPQQVQMAFFSAFALIDKVDAYKKLNMSICHRRQKLLFEALELPINENKNNAA
                     YYTQFDIEEWAKLNYGEDLFKFISKNASPVDVLYKLANDYSVVLLSGNGFYGPEWSIR
                     ISLANLYDEAYTKIGKAIREILNGYIVEWQKIKK"
     gene            2240..3235
                     /locus_tag="L3M60_003214"
     CDS             2240..3235
                     /locus_tag="L3M60_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBN6227846.1"
                     /translation="MSIKKKVAIVFTGGTISMTVDDKVGAAIPTLSGEQILSMVTNID
                     KVADVEVFNFDEIPGPHITPYRMMELKNYVNDLLARKDITGVVITHGTDSLEETAYFL
                     DLTIDNIKPVIVTGAMRSSSELGYDGSSNLSASVCTAVSKDAMGKGVLVVLNNEVLLA
                     SEATKTNTLSLNTFKSLTSGPLGIIDCNELVLTRDIVNRTIIDTDKVESKVALFKAYV
                     GENADFIRFAVDSGYKGIVIEAMGRGNIPPQMLSGVEYAREKGLPVVIVSRCHSGRVF
                     DSYGYFGSGRDLKNLGCIFGGDLPGQKARIKLILALGKTNNLDEIKDFFEKGIYC"
     gene            complement(3422..3511)
                     /locus_tag="L3M60_003215"
     CDS             complement(3422..3511)
                     /locus_tag="L3M60_003215"
                     /inference="COORDINATES: protein motif:HMM:NF024835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227847.1"
                     /translation="MPPMATMKKICLALDCNIEDIVEIKKDED"
     gene            complement(4014..4208)
                     /locus_tag="L3M60_003216"
     CDS             complement(4014..4208)
                     /locus_tag="L3M60_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227848.1"
                     /translation="MKGGEYMKTLMFVFVIMYYITIVIKSITTIVKSITRLLDSISRL
                     KESLHKLRNRPDGNQDDFNK"
     gene            complement(4403..5074)
                     /locus_tag="L3M60_003217"
     CDS             complement(4403..5074)
                     /locus_tag="L3M60_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227849.1"
                     /translation="MKKYIRYQSRSELKQLRKLFEYKIDDIVGYLFCSRRTFERIERD
                     NGTSNICLANRIAELYFQRFDELFIEVDLEVIKFINSIREYTLSIEDSYIIENEIYYC
                     IFLKKLDTCRDQIWGSTYWLEDYNKYKDIRKLREANVAYLLKNTNHKITIINTENQWY
                     RIYFGLCIGKFHEILISKSCAEKCVPYILKRHVVESSLLFNNSKISDLMLLGKYDDKR
                     NILID"
BASE COUNT         1923 a          722 c          696 g         2003 t
ORIGIN      
        1 ttaaatgata atttaaaaat attttttaca ttctcatcaa ggcaacaata tatactttat
       61 tatttttatt gaaatttata ttttatatta ttttatttac tttatttact caaaatacat
      121 ttttttgact ttatttaaaa ttattaagat taataaatta ataaaataca cctcttacta
      181 aataacttta atatagagga aagttttact ttatttttat tcaaaattag aaaattatac
      241 acactataat aaaatacaat aaaaaactaa gctgataatt aaattgaaga tttattttat
      301 ataacaatca ttttttatta aaaagcattt tttgttttaa tatatattta ataaagttaa
      361 atacagttat tattttaatt tcatacttta ctttttctat aaacctatta taattaaagt
      421 ataatcattt ttgtaacttg tttattttat tataaaaatg tgaaacttat tacataagga
      481 ggaatttaac atgaaaaatc atacttataa agaaatcaaa aacatatatg gaaaaataag
      541 cccttttgaa tttaaggaca aattaataga tattgcaaaa tatactgcaa aggaaaataa
      601 cagggaatta cttgatgcag gtcgtggaaa tccaaactgg acttgttcaa ctgccagaga
      661 agcctttttt acttttggtc atttcgccat taatgaaact cgttctaatt gggatttagg
      721 tcatttagct ggaatgcctc aaaaaaaggg tattaaagaa agatttttta aatttataaa
      781 tgaaaatata gatatgcctg gagcatattt agctagagat attataaact ttggtataaa
      841 tgaacttgga tttgatggag atgagtttat tcatgaacta gctgatggaa ttattggtga
      901 taactatcca cttccagata gaatgttacc tcatatggaa aaaatagtac acgattacct
      961 agttcaagaa atgaaatatg atatatcagg taaatatggt gatgtagaaa tttttgctgt
     1021 tgaaggtgct acagctgcta tgtgctatat ttttgattca ttaatggcaa atgaacttct
     1081 aaaaaaagga gataccatag ccttaatgac tccaatattt acaccttatc ttgaaatacc
     1141 taaccttcct cgttatgact ttaaagttgt aaatataaat gctaatgaag ttgacgaaaa
     1201 aggtgctcac acatggcagt atacaaaaaa agaattagag aaacttcgtg acaagtccat
     1261 taaagcttta ttcgttgtta atcctaataa tcctgcttct atagctatgg atgaaacatc
     1321 ttgtaataat ctaatagatg taattgaaaa ttataataaa gatttgatga taatatcaga
     1381 tgatgtatat ggaacttttg tagaggattt ttcgtcgttg atgtcaaagc taccttacaa
     1441 cactgttgga gtttattctt attcaaaata ctttggagtt acaggatgga ggcttggtac
     1501 gtttgctctt cacaagaaaa atgtttttga taagaaaata aatgatttaa ctggggaact
     1561 aaaaaaatca gtagataaac gttacagtga tatgagtctt aacccttcta gtctttcatt
     1621 tatggaaaga gttgttgctg atagtagact tgttgctctt aaccatactg ctggtctttc
     1681 tactccacaa caagtacaaa tggcattttt ctctgctttt gcattgattg ataaagtaga
     1741 tgcctacaag aaacttaata tgagtatctg ccatagacgt caaaaactat tatttgaagc
     1801 tttagagctt ccaattaatg aaaataaaaa taatgctgca tattacactc aatttgatat
     1861 tgaagaatgg gctaaattaa actatggaga agacttattt aaatttataa gtaaaaatgc
     1921 atctccagta gatgtacttt ataaattagc taatgactat tcagtagttt tacttagtgg
     1981 aaatggattc tatggtcctg aatggtcaat tagaatatct ctagcaaatc tatatgatga
     2041 agcttatact aagataggaa aagccataag agaaatacta aatggttata ttgttgaatg
     2101 gcaaaaaatt aagaaataat tatattacac tgaaatatct aaattatatg aataaaagaa
     2161 tttatgtaga ccttaactac tttttttgat ataatcctat cggaattata aattttattt
     2221 atgaaaggca gagaatagta tgagtattaa gaaaaaagtt gccatagttt ttactggtgg
     2281 aactatatct atgacagtag acgataaagt cggagctgct ataccaactt tatcagggga
     2341 acaaatatta tcaatggtta ccaacattga taaagttgca gacgttgaag tttttaactt
     2401 tgatgaaatt ccaggacctc atataacacc ttatagaatg atggaattga aaaattatgt
     2461 aaatgattta ttagcaagaa aagatattac aggtgttgta ataacgcatg gtacagatag
     2521 tttagaggaa acagcttatt ttctagacct tacaattgat aacatcaaac ctgtaatagt
     2581 cacaggtgct atgagaagta gttctgaact tggatatgat ggttcaagta atttatctgc
     2641 atcagtttgt actgcagtat ccaaagatgc tatgggaaaa ggagtccttg tagttttaaa
     2701 caatgaagta ctattagctt ctgaagctac taaaacaaat actctttctc taaatacatt
     2761 taaatctcta actagtggtc cattgggaat aattgattgc aatgaacttg tccttacaag
     2821 agatattgta aatagaacaa ttatagatac tgataaggta gaatctaagg ttgctttatt
     2881 taaagcatat gtaggagaaa atgctgactt tataagattt gctgttgaca gtggctataa
     2941 aggtatagtt attgaggcaa tgggtagagg taatattcct cctcaaatgc tttctggagt
     3001 tgaatatgct agagaaaaag ggcttcctgt tgttatagtt tctaggtgtc attcaggtag
     3061 agtattcgat agttatggtt actttggttc tggtagagat ttaaaaaatc ttggctgtat
     3121 atttggtgga gaccttccag gtcaaaaagc tagaataaaa cttatcttag cacttggaaa
     3181 aactaataac cttgatgaaa tcaaagactt ctttgaaaaa ggaatctatt gctaattaaa
     3241 ataatatctt ttatgatttt atgtattttt tcattgatat ttcaacattt aaaaatcaaa
     3301 gtccaatatt tatgtcagta aaatatttta tccacaattt ttatataagt ttcccacaac
     3361 ttatccacaa tattacccac aaaaaaaggt agcacttata ctagtagcta ccttttactt
     3421 attaatcttc atctttttta atctcaacaa tatcctctat gttacagtct aacgctaaac
     3481 atatcttttt cattgttgcc ataggtggca aaaaattgat actccatttt ttactaaata
     3541 gtactatatt ttttattaat catattgtcc caaactctta aaatatgctt ctaattcatt
     3601 taattttttt aatctacttt cttatcatcc tgctccatag tttcaacaat ctctcttgat
     3661 gttaatacct tgtctgattc ctaaatatat cttataactt ttaattctgc ttctggtaat
     3721 ttactaattt tcatagtctg cacctttttt tctaaattct tacatactaa atcatatgta
     3781 atattataac aaatattttt tataataaat tagaatattc aaattatatt ttcctaattt
     3841 attccatata aaaataattt acaactatat aaaattgtca atgttgtcag tattgtcatc
     3901 attgacgatt tgatatacaa taaaggtatg aaatagtatc aaaaacaaaa aaagaaacta
     3961 caatctatta gcctagagcg aagtttcaaa ttcaaaataa tttaagtaat gatttattta
     4021 ttgaaatcgt cttgattgcc gtcaggacga tttcttagtt tatgtaaaga ttcttttaat
     4081 ctacttatag aatccaataa tcttgttata gactttacta ttgttgtaat agattttatt
     4141 acaatagtaa tataatacat tataacaaat acaaacataa gtgttttcat atactcaccc
     4201 cctttcatac gaaggtgatt gtacctgaaa actttcgccc ttagattgta gtctcttgca
     4261 agattattct tgaattttaa ttataacata attttataat taccaaatat ttattcttgt
     4321 ttattcttaa ttaatttata tattttttat atccttgatt ccaaaaaata aatattaccg
     4381 ttttatttat aaaatgtaat gtttagtcta tcaaaatatt tcgtttatca tcatatttac
     4441 ctaataacat aaggtctgat attttagagt tattaaacaa taaacttgat tcaactacat
     4501 gtctttttaa tatatacggc acacatttct ctgcacaact tttagaaata agaatttcat
     4561 gaaatttacc aatacataag ccaaaatata ttctatacca ctgattttct gtatttataa
     4621 ttgttatttt gtgattagta tttttaagta aataagctac attcgcttct cttaatttgc
     4681 gaatatcttt gtatttatta taatcttcta accaatatgt acttccccat atttggtctc
     4741 tacatgtatc taattttttt agaaaaatac aatagtatat ttcattttct ataatataac
     4801 tatcttcaat acttaaagta tattctcgaa tagaattaat aaatttaata acctccaagt
     4861 caacctcaat aaacagttca tcaaatcttt gaaaatacaa ttcagcaatt ctattggcta
     4921 aacatatatt actagttcca ttatctctct cgattctttc aaatgttctt ctactacaaa
     4981 ataaatatcc tacaatatca tcaatcttat attcaaataa cttacgaagt tgtttaagtt
     5041 cacttcgact ttgatatctt atatactttt tcatatttta ttatcacctc ctgattttag
     5101 tttaatagtt ttattctttt tttactacga cattttattt ttgtagattt tatacatgaa
     5161 cattagcatt atcaactctt gcagcttttt tctaaaaggt cccctgttgc taaaaacttt
     5221 aaaaatatat tactttttta agaatataaa tattattact tcttatattt gaaaatcacc
     5281 tttcaaaatg aagaatactc tctttgataa cagttgattt agcaaagata tattgatagt
     5341 acac
//
LOCUS       DAFNNB010000185         5252 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0190, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000185 DAFNNB010000000
VERSION     DAFNNB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0190"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            39..770
                     /locus_tag="L3M60_003218"
     CDS             39..770
                     /locus_tag="L3M60_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBN6227850.1"
                     /translation="MNIYAHRGFSGKYPENTILAFKKCLEIDIYGIELDVHRTKDGKI
                     VVIHDEKVDRTFNGHGFVKDFTLKKLKTLNSSFEGYQNNKECKIPTLEEVLILISPTD
                     LILNIELKTDKVNYPNIEKDVLELILKYNIKNRVLISSFNSNSLKNFHKLDPSVKTGL
                     LCYLPINNVVNFAKFLGNSYLHPPLVLVNESLIEICHKNLLGVNVYTVNEEDDILHCL
                     KLNVDGIFTNYPDIASNLLQSKQYR"
     gene            850..1485
                     /locus_tag="L3M60_003219"
     CDS             850..1485
                     /locus_tag="L3M60_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBN6227851.1"
                     /translation="MDNLLNELEALKSNLDNLPIEKIENYDLSKTALFIIDVNNGFAK
                     QGALYSPRVESLIKPIEMFTKKISNKLNRVIAFTDSHTSKSIELLSYPVHCLENDIES
                     ELVDELKSIENLKILPKNSTNGFFALENLDFDNIDNIIIVGDCTDICIYQFAITLKSY
                     FNQHNIEKNIVVPMNLVDTYDIPNVHPAEILNLVFFNSMIQNGVNVLKEIR"
     gene            complement(1737..2330)
                     /locus_tag="L3M60_003220"
     CDS             complement(1737..2330)
                     /locus_tag="L3M60_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3867 domain-containing protein"
                     /protein_id="HBN6227852.1"
                     /translation="MSDDRIIDFNELKNKVKDSDVDKFEQYIYNLYFSVMDGKMSMAE
                     FSRKIFDYMRDNNISQEKFMKIQKQFMERYGMDTEEVEKQLRNFGIDPSTAGFMSNNT
                     SSKVSTEDLESFKKSAGFYEKYGEKIQPKSCITTFIKNDLNDINVIIDQEKIMLCSDR
                     KINLMDSELNEFLLEYKNMFNKKIKVVMCETTNKYDY"
     gene            complement(2401..3264)
                     /locus_tag="L3M60_003221"
     CDS             complement(2401..3264)
                     /locus_tag="L3M60_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HBN6227853.1"
                     /translation="MLGIIAPSGPLRNTSLEEIKFNLESYGYEVKFSESCSLSYKGYL
                     AGNDDIRARDIEGMFLDKDVDMIMCLRGGYGTTRILDKINYEIIKQNPKPFIGFSDIT
                     GLNLAFYKNCGLLPYHGIMAANVAKWDEFTYKSLVNALEFKDDLYLENPKEEKIYTVC
                     EGKAEGIIMGGNLSLIIATMGTKYEIDAKDKILFIEEIGEPQYKLDRMLTQLYSSGKL
                     EECNGIIFGDFKDCIEEKDLIELLIEFANKVNKPSIYNLQSGHCIPMITIPLGRMCEL
                     DATEKIVKLKK"
     gene            3427..4596
                     /locus_tag="L3M60_003222"
     CDS             3427..4596
                     /locus_tag="L3M60_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6227854.1"
                     /translation="MLNIRTLCNEINDWVINIRRDLHKTPELGLEEFQTKKKIIKYLN
                     EIGINYIEYKNHTGITAYINVNPNFETVAIRADIDALPITEELNYSYKSINIGKMHAC
                     GHDAHTAILLGTCNILFKLKDYLNVNVKFFFQPAEETIGGAQLMIEDGCLKNPNVKYI
                     FGLHVNPNINKNLIELKYNTLNASTDTLQLTVHGSKCHGAYPHQGIDAIVISAHIITA
                     LQTIVSRNTNPTDSVVISLGKIKGGIKENIVCDKVVIRGTLRTLTPETREFSKKRIKE
                     ICDFTCKTFGGSISVEIEEGYPALINSNYLVDYVKQNAVELFGKENIILKDAPTLGAE
                     DFSYFLKHCEGAFYHLGCANREKNIISPLHTSTFDIDEGCLITGVMLHVKNVLAF"
     gene            4697..5173
                     /locus_tag="L3M60_003223"
     CDS             4697..5173
                     /locus_tag="L3M60_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HBN6227855.1"
                     /translation="MKEILINPIGKIRIQGEEVFIKLDKKYVPALNELDDFSHLNVFW
                     WADGFDNPELRSILETPKPYKSGPDVIGIFATRSPIRPNPIALTTVQIISIDHENGII
                     KIPYIDANDNSPVIDLKPYTPSIDRVENPSVPKWCSHWPKSVEKSGEFNWEDEIEF"
BASE COUNT         1900 a          768 c          661 g         1923 t
ORIGIN      
        1 tataagataa gttatattta ttgaaaggag ccacataaat gaatatttat gctcatagag
       61 ggtttagtgg aaagtatcca gaaaatacta ttctagcttt taaaaaatgt ttagagatag
      121 atatatatgg aatagaactt gatgtccaca gaactaagga tggaaaaatt gtagttattc
      181 atgatgaaaa ggttgataga acttttaatg gacatggttt tgtaaaagac tttactttaa
      241 aaaagttaaa gactctaaac tcatcttttg aaggatatca aaacaataaa gaatgtaaaa
      301 ttccaacttt agaagaagta ttaattctaa tttcacctac agaccttatt ttaaatatag
      361 agctaaaaac tgataaggta aactatccca atatagaaaa agatgtttta gaacttattt
      421 taaagtataa tataaaaaat agagttctta tatctagttt caacagcaat agtttaaaaa
      481 actttcataa attagaccct agtgtaaaaa caggattatt gtgttattta cctataaaca
      541 atgtagtcaa ttttgctaaa tttctgggta atagttacct tcatccacct ctagttttag
      601 taaatgaatc tctaattgaa atttgtcata aaaatttact tggtgtcaat gtatatactg
      661 taaatgaaga ggatgatata ctacactgtc taaaactcaa tgtagatggt atttttacta
      721 actatccaga cattgcttca aatcttttac aatcaaaaca atatcgctaa ttacacaata
      781 gtttgcactc cttgactttt atataattaa tatgtacaat tttattaaat ttatcttgga
      841 ggtataacta tggataactt attaaatgaa cttgaagctt taaagagtaa tttagataat
      901 ctaccaatag aaaaaataga aaattatgac ttgagtaaaa ccgctttatt tataattgat
      961 gttaataatg gctttgcaaa acaaggtgca ttatactctc ctagagtaga gtctttaata
     1021 aagccaatcg aaatgtttac aaaaaaaata tcaaacaaat taaatagagt aatagcattt
     1081 actgattctc atacttctaa gtcaatagaa cttttgagtt atcctgttca ctgtttggaa
     1141 aatgatatag aaagtgaatt ggttgatgaa cttaaatcga tagaaaacct aaaaattctt
     1201 cctaaaaatt ctactaatgg attttttgca ttagaaaatt tggattttga taatatagac
     1261 aatataatta tagttggcga ttgtacagat atatgtatat atcaatttgc aataactcta
     1321 aaatcatatt ttaatcagca taatattgaa aaaaatatag ttgtacctat gaatttagta
     1381 gatacttatg atattcctaa cgttcaccct gctgagattt taaacttagt attttttaat
     1441 agtatgattc aaaatggagt taatgtatta aaagagattc gttaatactc taatactttg
     1501 catcacatat gatacaatta tcagataact atttttaata aaacctctaa tagaaaaaca
     1561 cagttttaat gataagatta caatttaaaa atattatcta ctatgactaa tcttcacaat
     1621 ataaaaaatc gcagtttaga tactccttat aaaaatttat aaggaataat aaattgcgat
     1681 ttttattttt aaataatacc attaatctat actacttcag tatttaaatt cactttttaa
     1741 tagtcatact tatttgtagt ttcgcacatt actaccttta ttttcttatt aaacatattc
     1801 ttatattcta ataaaaactc atttagttct gaatccataa gatttatttt tctatcacta
     1861 caaagcatta tcttttcttg gtcgattatt acattaatat catttaagtc attttttata
     1921 aatgttgtta tacaactctt tggttggatt ttttctccat atttttcata aaatcctgca
     1981 ctcttcttaa atgactctaa atcttctgta gatactttgc tggaagtatt attactcata
     2041 aaacctgctg tagaagggtc gattccaaag tttctaagtt gtttttctac ttcttcagtg
     2101 tccataccat atctctccat aaattgcttt tgtattttca taaatttttc ttgagatatg
     2161 ttgttatctc tcatataatc gaatattttt cttgaaaact cagccataga catctttcca
     2221 tccataacag aaaaatataa gttatatatg tattgctcaa atttatctac atcagaatct
     2281 ttaactttgt tctttagttc gttaaagtct attattctat catcagacat ttttcatcct
     2341 cctagctaaa tttttacttg tataagatta taccatattt ctattatatt tatatacaca
     2401 ctattttttt aatttaacga ttttttctgt agcatctaat tcgcacattc tgcctaaagg
     2461 aatagttatc attggtatac aatgtcctga ttgaagatta tatatagaag gtttattgac
     2521 tttatttgca aattctatta gtaattctat taaatctttt tcttcaatac aatccttaaa
     2581 atctccaaaa attataccat tacattcctc aagctttcct gaggaatata gttgtgttaa
     2641 cattctatcc agtttatatt gaggttctcc aatttcctca ataaacaaaa ttttatcttt
     2701 tgcatctatt tcatattttg ttcccattgt agcaattatt aaggataaat ttcctcccat
     2761 tattattccc tctgctttac cttcacatac agtataaatt ttttcttctt ttggattttc
     2821 taaatataag tcatctttaa attctaaagc atttaccaat gatttatagg taaattcatc
     2881 ccatttagct acattggcag ccattattcc atgatatggt aaaagaccac aatttttata
     2941 aaaagctaaa tttaaacctg ttatatcaga aaaacctata aatggttttg gattttgttt
     3001 tattatctca tagtttattt tatccaatat tcttgttgtt ccataacctc ctcttaaaca
     3061 cataatcata tctacatctt tatctaaaaa catcccttct atatctcttg ctctaatatc
     3121 atcattacca gctaaataac ctttataact taaactacaa ctttcagaaa acttaacttc
     3181 gtaaccataa ctttctaagt taaactttat ttcctccaaa cttgtatttc ttaatggccc
     3241 agatggcgca attattccta acatttaaac acctcttctt ataaaatgat atattcttct
     3301 ccatactaaa tatctctaat ataattataa gatattttat ttgtacttga tacagaataa
     3361 tgatttacat aaaataatat aattaataag gaaaaataaa tttttaatat ggttaggatg
     3421 tgttttatgc ttaatataag aactctttgt aatgagataa atgattgggt tataaatata
     3481 agaagagatt tacataaaac tccagaactt ggactagaag aatttcaaac taagaaaaaa
     3541 attattaagt acttaaatga aataggtata aattatattg aatataagaa tcatacaggg
     3601 ataactgcat acatcaacgt aaatccaaac tttgaaacag ttgcaataag agctgatata
     3661 gatgccttac ctataacaga ggaacttaat tattcttata aatctattaa tattggaaaa
     3721 atgcatgcat gcggtcatga tgcacacact gctattttac taggtacttg taatatacta
     3781 tttaaattaa aggattactt aaatgtaaac gtaaaattct tttttcaacc tgctgaagaa
     3841 actattggtg gagctcaact tatgatagaa gatggatgtc ttaaaaaccc aaatgtaaaa
     3901 tatatatttg gacttcatgt aaatcccaat atcaacaaaa atctgattga attaaaatac
     3961 aacactctaa atgcttctac cgatacactt caattaactg tacatggttc aaaatgtcat
     4021 ggagcttatc cacatcaagg tattgatgct atcgttatat ctgctcatat tattacagct
     4081 ctacagacaa tcgttagtcg taacacaaat ccaactgatt ctgtagttat ttctctagga
     4141 aaaattaaag gtggaatcaa agaaaatata gtttgtgaca aagtagttat aaggggtact
     4201 ttgagaacct tgactcctga aactagagaa ttttcaaaaa aacgaattaa agaaatttgt
     4261 gattttactt gtaaaacatt tggaggcagt atttccgttg aaattgagga aggttaccca
     4321 gctcttatca attcaaacta tctagtagat tacgtcaagc aaaatgctgt agaattgttt
     4381 ggaaaagaaa atattatcct taaagacgca cctactcttg gggctgaaga cttttcgtat
     4441 tttctaaaac attgtgaagg agctttttac caccttggtt gtgcaaatcg agaaaaaaac
     4501 attatttccc ctctgcatac ctctactttt gatattgacg aaggttgtct tattactggt
     4561 gtcatgcttc atgtaaaaaa tgtattagca ttttaaatat taatattttt atttattatt
     4621 ttatattgac cctcacatga tgtcatgtta taccctaaaa ttacaaatga taaattaatt
     4681 aattggaggt aatattatga aagaaatatt aataaatcct ataggtaaaa tacgtattca
     4741 aggcgaggag gtctttatta aattagacaa aaagtatgta cctgcattaa atgaactcga
     4801 cgacttcagc cacttaaatg tattttggtg ggctgatggg ttcgataatc ctgaactaag
     4861 aagtatttta gaaactccta aaccttataa aagtgggcca gatgttatag gaatatttgc
     4921 aacaaggtca cctataagac ccaacccaat agcattgact actgtacaaa ttatcagtat
     4981 agaccatgaa aatggtataa taaaaatacc atatattgat gctaatgata atagtcctgt
     5041 tattgactta aagccatata ctccaagcat tgatagggtt gaaaatccgt cagttccaaa
     5101 atggtgtagt cactggccta agagtgtaga aaaatcagga gaatttaatt gggaagatga
     5161 aattgaattt taaattactt ttttcttgat ttcattaact tgttttccac atttatttta
     5221 tttacactta ccaagatttt atgtcacata ta
//
LOCUS       DAFNNB010000186         5219 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0191, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000186 DAFNNB010000000
VERSION     DAFNNB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0191"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(214..1398)
                     /locus_tag="L3M60_003224"
     CDS             complement(214..1398)
                     /locus_tag="L3M60_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBN6227856.1"
                     /translation="MLSKRLNFITPSYTIGISSKVKEMENNGIKVINLSIGEPDFNVP
                     NNAKSYGIDSLNKDYTKYDLVPGLKILREEICKKLIEENNCKYSIDEIVVSSGAKNSI
                     TNTLLALTDEGDEVLLPKPYWVSYPEMVKLVNAVPIFIDTKKENDFKLTKEELEKSIT
                     DKTKILVINNPSNPTGSVYTKDELIEIVDVCIQNKIYILADEIYEKICYSGEFTSIAS
                     LSEEAKDITITINGFSKSAAMTGLRLGYTASNKAIAKAMSSIQGHLISHPSLTAQYIA
                     YGALKDCSTDIDNMVKTYKSRRDLIKSKLDSIENIGYVNPNGAFYAFIDLSKVSEKFE
                     YKDSFSIEFCNQFLEEYNVAVVPGIAFGMDKYIRISYACSENTFLSGLNKLKEFVYKI
                     MA"
     gene            1771..4122
                     /locus_tag="L3M60_003225"
     CDS             1771..4122
                     /locus_tag="L3M60_003225"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside triphosphate reductase"
                     /protein_id="HBN6227857.1"
                     /translation="MVEFVKKRDGRVIPFNEDRITRAIFLAATNVAEREGIVPDYKLS
                     EQLTQEVIKFLNHKYSESVPSVEDIQDSVVKVLIETGHAKTSEEYIIYRTERSRIRNS
                     KTRLMKAIEEITFEDAEDADIKRENANINGNTAMGTMLQYGSTVSKEFCKTHILKPEH
                     SFAHDNGDIHIHDMDFLNMGTLTCCQIDVKKLFNGGFSTGHGFLREPQDIISYGALAA
                     IAIQSNQNDQHGGQSIPFFDYGLAEGVYKTFKKFYIGNLAKALKLFKGIENNDVIKNI
                     VYNTEKETNQKVGLKRDELYLNLEKEKLIQTFDIDEELVNKMQNFAFEESYKETDKKT
                     YQSMEAFIHNLNTMHSRAGAQVPFSSVNFGTDTSEEGRMVTKNLLLSQERGLGNGETP
                     IFPILIFKVKEGINLNPEDPNYDLFKLSCRVSAKRLFPNFSFLDAPFNAKYYKKGEPD
                     TEATYMGCRTRVLSNVCGSETVSGRGNISFTTVNLPRLGIKHGIINNEKANLDGFFEE
                     LDEKINLIIEQLLERLEVQGNKKMKNFPFLMGQGVWKGSDELGPEDTLKEVIKQGTLT
                     IGFIGLAECLIALIGKHHGESKEAQELGLKIVSHMRDKMDEATDKYKLNFSLMGTPAE
                     GLSGRFTKIDKKVYGEIKGITDKEYYTNSFHVPVYYNISAYDKIEIEAPYHELTNAGH
                     ITYVELDGDPSDNLEAFETVIKAMKDLGIGYGSINHPVDRDPICGFSGVITSNICPVC
                     GRNEDESDIKFERIRRITGYLVGTVDRFNNAKKAEVRDRVKHR"
     gene            4144..4683
                     /gene="nrdG"
                     /locus_tag="L3M60_003226"
     CDS             4144..4683
                     /gene="nrdG"
                     /locus_tag="L3M60_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HBN6227858.1"
                     /translation="MKIRMSSTISYDSIVDGPGLRMVIWTQGCIHNCKECHNPQTHDL
                     CGGFYMDTEEIINKVKSLKLQKGITLSGGEPFLQPEPLEVIAREAKTNGLDVWSYTGF
                     TFEQLLDKRNSAYFKNLNLLKQIDILVDGKFVAEKKDISLKFRGSSNQRIIDVQKSLK
                     YKKVFLVEQYMKDDLSIAE"
BASE COUNT         2024 a          582 c          829 g         1784 t
ORIGIN      
        1 taataaattt agaatttaac ctatatagtt taacttaata atttaatgaa aaaaataaaa
       61 gacttactga tttttaaatc tataagtctt ttatttttta tttaatattt aaattaagtt
      121 gttgaatata tcttatatta cttactttat atattgatta tcattaggtt aagacccata
      181 tattgtatca attactattt atttaaattg ttattatgcc attattttat atacaaattc
      241 ttttaattta tttaatccag ataagaatgt attttcactg caagcataag atatacgaat
      301 atatttatcc ataccaaatg ctataccagg aacaactgca acattatatt cttctaaaaa
      361 ttgattacaa aattctatag aaaaactatc tttatattca aatttctcag atactttaga
      421 aaggtctata aatgcataga atgcgccatt tggattcaca tatcctatat tttcgataga
      481 gtctagtttt gatttaatta aatctcttct agatttatac gttttaacca tattgtctat
      541 gtcagttgag caatctttca aggctccata agctatgtat tgtgctgtca aacttgggtg
      601 agatataaga tgtccttgta tagaactcat tgctttagca atcgctttat tagatgcagt
      661 ataccctaat cttaatccag tcattgcagc tgacttagaa aaaccattta tagttattgt
      721 tatatcttta gcttcttcgc ttaatgatgc aattgatgta aattcgccac tgtagcatat
      781 tttttcatat atttcatctg ctaaaatata tattttattt tgtatacaca catctactat
      841 ttctattaat tcatcttttg tataaacact accagtagga tttgaaggat tgtttattac
      901 aagaattttt gttttatctg ttatagattt ttcaagttct tcttttgtca gtttaaagtc
      961 attttctttt ttagtatcaa taaaaattgg aacagcatta actaacttaa ccatttcagg
     1021 ataactaacc caataaggtt ttggcaataa gacttcatct ccttcgtctg ttaaagctag
     1081 taaagtattt gttattgagt tttttgctcc gcttgataca actatttcgt ctattgaata
     1141 tttacagttg ttttcctcaa taagtttttt acatatttct tctctaagta tttttaatcc
     1201 tggaactaaa tcatatttag tgtaatcttt atttaaagaa tcaataccat aagattttgc
     1261 gttattaggt acattaaaat caggttctcc tatactaaga tttattactt taattccatt
     1321 gttttccatt tctttaactt tagaactaat acctattgtg taggatggtg ttataaagtt
     1381 tagtcttttt gataacataa aatgcctcct tttaaattat tatttatatt atacgacaat
     1441 tttaaagaat ttacataagg aaatttgtag atatatacat taatatatat aatagaatac
     1501 actttttgcc taaaattagt gtaaaaaaaa gagaaaaaaa ttttgacttt caaagttgtt
     1561 agaaacgcta ctgttcaaaa tatccacata gtatttgcaa tgatttgcgc aagtttgact
     1621 attatgtaca aatttggtaa tcttaatata gtaattatta agatagtccc tttaacttag
     1681 ttgaagggga tttttttaga cttaatatac tacatatagt ggtatgtgga ttaacaaaat
     1741 acaatatata gtaacaggag gtttttttaa ttggttgaat tcgtaaaaaa aagagatgga
     1801 agagtaattc catttaatga agatagaata acaagagcta tatttttagc agcaacgaat
     1861 gttgcagaaa gagaaggaat agtacctgat tataaattat ctgagcaatt aactcaagaa
     1921 gtaataaagt ttttaaatca taaatactca gaatcagttc caagtgttga agatatacaa
     1981 gattctgtag ttaaggttct tatagaaact ggccatgcaa aaactagtga agaatatata
     2041 atatacagaa ctgaaagaag tagaataaga aattcaaaaa caagattgat gaaggcaata
     2101 gaggaaatta cctttgaaga tgcagaagat gctgatataa aaagagaaaa tgcaaatatt
     2161 aatggtaata cagcaatggg aactatgtta caatatggaa gcactgtatc taaagaattt
     2221 tgtaaaactc atatattaaa accagaacat tcttttgctc atgataatgg agatatacac
     2281 atacatgata tggatttttt aaatatggga acattaactt gttgtcaaat agatgttaaa
     2341 aaactattca atggtggatt ttcaacagga catggttttt taagagagcc acaagatata
     2401 ataagttatg gagcattagc tgcaattgct atacaaagca atcaaaacga tcaacatgga
     2461 ggtcaaagta taccattttt cgattatgga ttggctgaag gtgtatataa gacatttaag
     2521 aaattctaca taggaaattt agctaaagcg ttgaagttat ttaaaggaat tgaaaataat
     2581 gatgtgataa agaacatagt atataataca gagaaagaaa caaatcaaaa agttggtctt
     2641 aagagagatg aattgtatct aaacttagaa aaagagaaat taatacagac ttttgatata
     2701 gatgaggaac ttgtaaataa gatgcaaaac tttgcatttg aagaatcgta taaagaaact
     2761 gataaaaaaa catatcaatc tatggaagca tttatacaca atctaaacac aatgcactct
     2821 agagcaggtg cacaggtacc attttcaagt gtaaacttcg gaacagatac ttctgaagaa
     2881 ggaagaatgg taactaagaa tcttctatta tctcaggaaa gaggtttagg taatggagag
     2941 acaccaattt ttccaatttt aatatttaag gtcaaagaag gcataaattt aaatcctgaa
     3001 gaccctaatt atgatttatt taaattatca tgcagagtat cagctaaaag actattccct
     3061 aattttagtt ttttagatgc accttttaat gcaaaatatt ataaaaaagg tgaaccagat
     3121 acagaggcta catatatggg ttgtagaaca agagtactta gtaatgtgtg tggttcagaa
     3181 accgttagtg gtagaggaaa catatctttt actacagtaa atttaccaag attgggtata
     3241 aaacatggta ttataaataa tgaaaaggct aatttagatg gtttctttga agaattagat
     3301 gagaagataa acttaattat agaacaatta ttggaacgtc ttgaggttca aggaaataaa
     3361 aagatgaaga attttccatt cttaatgggg caaggtgtat ggaaaggttc tgatgaatta
     3421 ggtccagaag atacattaaa agaagtaata aaacaaggaa cattgacaat tggatttata
     3481 ggtttagcag aatgtcttat tgctcttata ggtaaacatc atggagaaag taaagaagct
     3541 caagaattag gcttaaagat agtgtcacat atgagagata aaatggatga agcaacagac
     3601 aaatataaat taaatttctc tttaatgggt actccggcag aaggtctttc aggtagattt
     3661 actaaaatag ataaaaaagt ttatggggaa attaaaggta ttacagataa agaatactat
     3721 actaattcat tccatgtacc agtttattac aatataagtg catatgataa aattgagata
     3781 gaagctccat accatgaatt gactaatgca ggtcatataa catatgtaga gttagatgga
     3841 gatccatctg ataatctaga agcttttgaa actgttataa aggctatgaa agatttaggt
     3901 ataggatatg gaagtataaa tcatcctgta gatagagacc caatatgcgg tttttcaggt
     3961 gtaataacaa gtaatatatg ccctgtatgt ggaagaaatg aagatgaaag tgacatcaaa
     4021 ttcgaaagaa taagaagaat aacaggatac ttagttggta cagttgatag atttaataat
     4081 gctaaaaaag cagaagtaag agatagagta aaacatagat agttttattt gggggaataa
     4141 gttatgaaaa taagaatgtc atctactata agttatgaca gcatagttga tggaccaggg
     4201 ttaagaatgg tgatatggac tcaaggatgt attcataatt gtaaagagtg ccataatcct
     4261 caaacgcatg atttatgtgg aggattttat atggatacag aagaaattat aaataaagta
     4321 aaatcattga aattacaaaa aggtattacg ctatcaggag gagaaccatt tttacaacca
     4381 gaaccattag aagtaatagc gagagaagct aaaacaaatg gactagatgt atggtcatat
     4441 actggattta cgtttgaaca actgttagat aaaagaaata gcgcatattt taaaaattta
     4501 aatttactaa aacaaattga tattttagtg gatggaaaat ttgtagctga aaaaaaagat
     4561 ataagcttaa aatttagagg ttcatcaaat caaagaataa ttgatgttca aaaaagtttg
     4621 aaatataaaa aagtgttttt ggttgaacaa tatatgaaag atgatttgtc tatagcagaa
     4681 taaaatataa ataaacagga gtaaatatat gcttatattt actcctgttt atttgcatat
     4741 aatatttcaa gcttgtatga tgtaacaatc aaaccaaaat acttattgta tatctgaaat
     4801 taagtttttt atagtttaga ggtgttttat gtatattaca aagggtatac catacattat
     4861 ttaatcgatt cttatgccaa gaaacaatta atttacagaa atttttaatt gttacaatat
     4921 aaatatttag ataagttata aaatgttgat aaataattta gaaagtttat taatccaata
     4981 ttatgtatgt ttatattaag taatcaagta gtctaaaaaa tgaaaaaggt ttaaaataag
     5041 tattttaact aaatgtataa gagttaaatt tacatataag tatagattag atatataaga
     5101 aataattatg taatatctat ctttgtaaaa aaatattaaa ttttgtaatt tgtggataaa
     5161 tatgtttata atgtggataa tatttaatgt aataagttaa agcaatatga atcaactgt
//
LOCUS       DAFNNB010000187         5142 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0192, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000187 DAFNNB010000000
VERSION     DAFNNB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0192"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            56..385
                     /locus_tag="L3M60_003227"
     CDS             56..385
                     /locus_tag="L3M60_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBN6227859.1"
                     /translation="MIGVFTGFINGVFGSGGGTLLVPILNDIVKVEEHKSHATALSII
                     IFLTTTSSVLYVSKGTYDVNLTIKVAVGSILGGIIGAKLLNKVTGKFLRISFGIIMII
                     AALRMVF"
     gene            385..762
                     /locus_tag="L3M60_003228"
     CDS             385..762
                     /locus_tag="L3M60_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBN6227860.1"
                     /translation="MLFALIGFFAGIIGGMGMGGGTILIPALVLLAGIDTKIAQSVNL
                     LSSIPMTLIALAIHIKNKNVIFKLVIPIAVFGVLGAVFGSLLANYLSSDILKKIFGVF
                     LLLVGLFEVKKGFCAKSNKKPLN"
     gene            complement(848..1492)
                     /locus_tag="L3M60_003229"
     CDS             complement(848..1492)
                     /locus_tag="L3M60_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HBN6227861.1"
                     /translation="MSNYRTLHEFGTDEITIEKSVFIGYAKPIKSEEEALEFINEIKK
                     KHKDATHNVWAYTVGKNMNIQRYSDDGEPQGTAGIPTLEVIKKEDLRDVVVVVTRYFG
                     GTKLGAGGLVRAYTKGAKLGLESGKIIYKVMYQEVKVKIDYTQLGKVQNELMNLGYFI
                     KDTIYEDNVEIVVYSRLEDVDKLSEKMIDITSGTGKIVLGEEFYLSEQNGEILL"
     gene            complement(1801..2244)
                     /locus_tag="L3M60_003230"
     CDS             complement(1801..2244)
                     /locus_tag="L3M60_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HBN6227862.1"
                     /translation="MIVRRCAGGVVFYANKVLIVKNDRGEWTLPKGKILGGGLPYESA
                     VQRVKVETGIDAKMIDVAGDTMYEFFSRSRQQEVCNAIMWYVMEACNTDCVFAPEFQE
                     GGFYKVKDALEMLSHHKEQALVEVSYKKFKELKKLVSEGDDVPAN"
     gene            complement(2331..3008)
                     /locus_tag="L3M60_003231"
     CDS             complement(2331..3008)
                     /locus_tag="L3M60_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HBN6227863.1"
                     /translation="MDINWENIDNLEDYFITYLLYKESKTVSQISKIRNISSTEASEQ
                     LIQAKLKIKEMQKDDFEASKDILDKFLELDKIKRLDFMDSLDDEKMVYFKRKVFKRIL
                     VEKNAEDLIVLIWATGELKDDRFLKLLHQLTNHRHSDIRRITYSAIRKIESPSSREVL
                     QKGLYDKNAQTRQYCAKALSKLGDESSLKILQQLKDKNKNFEKEYVLRAYDEAIKSLE
                     NAKNLKK"
     gene            complement(3016..4299)
                     /gene="hflX"
                     /locus_tag="L3M60_003232"
     CDS             complement(3016..4299)
                     /gene="hflX"
                     /locus_tag="L3M60_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBN6227864.1"
                     /translation="MKEIQEKALLVGLNLTTIVKKNDDIDTSESMEELKELTKAAGAE
                     VVGSLIQNKHSIDAAYYIGKGKVEEIRAYSDSLDATLVIFNDELSGAQIRNIENVVGR
                     KVIDRTTLILDIFAQRALSKEGKLQVELAQLKYRLPRLYGMGGEMSRTGAGIGTRGPG
                     EQKLEIDKRHILNKAADIRRELKEVKKNRETQRVKRLKSNIPIVALVGYTNAGKSTLL
                     NELIKTHKDYEQEKEVFVKDMLFATLDVTLRKALLPNKKEFLVVDTVGFVSKLPHDLV
                     EAFKATLEEVQYADLILHVIDATNTSYELQKSTTEGVLKELGVNDKKHILVYNKVDKL
                     ELDIYPKSQEDIVYISAKQGINMDKLLNMIEIALMENTYSVSLMLPYERGDIFSRIKD
                     KYNVENFEYGENGITLDVDLDEEDFNIYREYILEK"
     gene            4516..4776
                     /locus_tag="L3M60_003233"
     CDS             4516..4776
                     /locus_tag="L3M60_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich small domain-containing protein"
                     /protein_id="HBN6227865.1"
                     /translation="MGENYKFFNHKDCEFFPCHKTNKPEEFNCLFCYCPLYALGENCG
                     GNFKYTDKGIKDCSSCMLPHKKDNYNYIMGKFQDLVKITSKK"
     gene            complement(4882..>5142)
                     /locus_tag="L3M60_003234"
     CDS             complement(4882..>5142)
                     /locus_tag="L3M60_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HBN6227866.1"
                     /translation="TSITLIMMPLLLTVPILLNFEKSFIIFHKLLFRNDYWIFNPNLD
                     PVINILPEEFFFHSGMMILILILLSSILLFVMYKLCKYFKIK"
BASE COUNT         1661 a          882 c          559 g         2040 t
ORIGIN      
        1 gaaaaaaact gatggtttaa agaaaaaata ctttgatttt aacttaaaaa atacgataat
       61 tggagttttt acaggattta taaatggagt ttttggttct ggaggaggca ctcttttagt
      121 tccaatatta aatgatatcg ttaaggtaga agaacataaa tctcatgcaa ctgctctttc
      181 tataataata ttcttaacga caacaagttc tgttttatat gtatctaaag gtacttacga
      241 cgttaattta acaattaaag ttgcagtcgg aagtattctt ggtggtatta taggagctaa
      301 attattaaat aaggttactg gaaagtttct tagaatttct tttggaataa ttatgataat
      361 tgcagcatta aggatggtgt tttaatgtta tttgctctaa ttggattttt tgcaggaatt
      421 attggaggaa tgggaatggg tggaggaaca atccttatac ctgcactcgt tttacttgct
      481 ggcatagaca ctaaaatagc acaaagcgta aatttacttt catctatccc tatgacactt
      541 atagcacttg ctatacatat aaaaaataaa aatgtaattt ttaaattggt tatacctata
      601 gctgtgtttg gggttttagg agctgtattt ggttcactat tagctaatta tctatcttca
      661 gatatattaa aaaaaatatt tggggtattt ttacttttag ttggtttatt tgaagtaaaa
      721 aaaggattct gtgcaaaatc taataaaaag ccattaaatt gatttaggtt aaatcaattt
      781 aatggctttt tacttaaaaa taccaattaa ttatttaaga tattgactat tacaataacc
      841 ttttagatta tagtaaaatt tctccatttt gttcagacaa ataaaattcc tctcctaaaa
      901 ctatttttcc agttccactt gttatatcaa tcattttttc tgataattta tcaacatctt
      961 ctaatcttga atatactact atctcaacat tatcttcata tatagtatct tttataaaat
     1021 accccaaatt cattaactca ttttgtactt ttccaagttg agtatagtct atcttaacct
     1081 taacttcttg atacattacc ttatagatta tcttgcctga ttcaagtcct aacttagcac
     1141 cttttgtata agctctaact agacctcctg cacctaattt tgttcctcca aaatacctag
     1201 taacaacaac cactacatct ctcaaatctt ctttttttat aacctctaat gtaggtatcc
     1261 cagctgttcc ttgtggttct ccatcatcac tatatctttg tatattcata tttttaccta
     1321 cagtataagc ccacacattg tgtgtcgcat ctttatgctt tttctttatt tcatttataa
     1381 actctaaggc ttcctcttca ctctttatag gcttagcata tccaataaaa acagactttt
     1441 caatcgttat ttcatccgtt ccaaattcat gtaatgtcct gtaattgctc attatacttc
     1501 ctcctatttt aatcattttc taatatacac atcaaataca tatttattta taaaattatt
     1561 ctataaaaaa ataaatggac ttttaatatt tctttaatca atatcataag acaattatat
     1621 aataaaattg tatacttttt aatttttatt ttaaatatag cttactgatt ttaaatgtaa
     1681 attactaaag ctctaaattg ataaatcata acttaaacaa gctatataat ttatgcttaa
     1741 aactgcttca attaaaaaag gaaggggcta tcccttcctc agaatcctct aaattcacaa
     1801 ttaatttgct ggaacatcat cgccctctga aactaatttc tttaattcct taaacttttt
     1861 ataagatact tctactaaag cttgctcttt atgatgtgat aacatttcta aagcatcctt
     1921 tactttataa aatccacctt cttgaaattc aggtgcaaat acacagtcag tgttacaagc
     1981 ttccataaca taccacatta ttgcattaca aacttcttgt tgtctacttc ttgagaaaaa
     2041 ttcatacata gtatcgccag ccacatctat cattttagca tctatgccag tttctacttt
     2101 aactctttga acagcactct catatggaag acctcctcct agtatctttc cttttggaag
     2161 tgtccattca cccctatcat tttttacaat aagtacttta ttagcataaa acactactcc
     2221 acctgcacaa cgtcttacta tcatttgaaa cccctcccat taatttattt attaccttgt
     2281 taaactttag aaataacatc attaagtaaa caaattgctt taataaaaaa ttactttttc
     2341 aaattttttg cattttctaa tgacttaatg gcttcgtcat aagctctaag cacatattct
     2401 ttttcaaaat tcttattctt gtctttcaac tgttgtaaaa tttttaaact gctttcatct
     2461 cctaattttg aaagagcttt tgcacaatac tgcctagtct gtgcattttt gtcgtagaga
     2521 cctttttgta gtacttctct actacttggt gattcaatct ttctgattgc tgaataagtt
     2581 attcttctaa tatctgagtg tctgtggttt gtgagctgat gaagcaattt caaaaatcta
     2641 tcatctttta gctcacctgt cgcccaaatt aaaacaatta aatcttcagc atttttttct
     2701 acaaggattc ttttgaaaac ctttctctta aagtatacca ttttttcgtc gtctaagcta
     2761 tccataaaat caagtctctt aattttatct agttctaaaa atttatctaa aatatcctta
     2821 gacgcctcaa aatcatcttt ttgcatttcc tttattttta attttgcttg tataagttgc
     2881 tcacttgctt cagtacttga aatatttcta attttactaa tttgagacac tgttttagat
     2941 tctttataaa gaagataggt tataaaataa tcttcaaggt tatctatatt ttcccagttt
     3001 atatccatat tcaacctatt tttctagtat gtactctcta tagatattga aatcttcttc
     3061 atctaaatct acatctagcg ttataccatt ttccccatac tcaaaatttt ccacattata
     3121 tttatctttt attctactaa atatatcacc tctctcataa ggtaacatca agcttactga
     3181 ataagtattt tccattaaag caatttctat catatttaat aacttatcca tatttatgcc
     3241 ttgttttgca gaaatataca caatatcttc ttgactttta ggatatatat ccaattctag
     3301 cttgtccact ttattatata caagtatatg ttttttatca tttacaccaa gttcttttaa
     3361 tacaccttca gtcgtacttt tttgcaattc ataactagta tttgtcgcat caattacatg
     3421 taagattaaa tctgcatact gtacttcttc caatgttgct ttaaacgctt ctactaaatc
     3481 atggggaagt ttacttacaa atccaacagt atctactact aaaaattctt ttttattcgg
     3541 caaaagagcc tttctcaagg ttacatctaa agttgcaaaa agcatatctt ttacaaaaac
     3601 ttccttttcc tgttcataat ccttgtgtgt ctttattaat tcgttcaaca gggtagattt
     3661 tcctgcattt gtataaccaa caagtgctac aatcggtata tttgacttta aacgcttaac
     3721 tctctgtgtt tctctattct tttttacttc tttaagttca cgccttatat ctgctgcttt
     3781 attaagtata tgtctcttat ctatttctag tttttgttct ccaggacctc tagtaccaat
     3841 tcctgcacct gttctactca tctcaccacc cattccataa agtcgtggta atctgtattt
     3901 taattgtgct aattcaactt gtaatttacc ttctttacta agtgcccttt gagcaaatat
     3961 atctaaaatt aaagtagttc tatctattac ttttctacca acaacattct ctatatttct
     4021 gatttgagca ccagataatt catcattaaa aattactaaa gtagcatcta aagaatcact
     4081 atacgctctt atttcttcta ccttaccttt tcctatataa taagctgcat ctatagaatg
     4141 tttattttga attaagctac ctacaacttc tgccccagca gcctttgtta gttcctttaa
     4201 ttcttccata gattcactag tgtctatatc atcatttttt ttcactattg tagtcaaatt
     4261 caaacctaca agaagtgctt tttcttgaat ttctttcact taatcacctc attttttgtt
     4321 ataaagcata ttctaattac aaaatacact tacattactt ttacattatt tttctgttga
     4381 ctaattatac cattaatact atgattaata atactatatt atcgactatt cataaaatta
     4441 tttagtataa ttatattatc accaaaatct tatatagtat aaaaaattta aattttattt
     4501 aattaggagg aagatatggg cgaaaattat aagtttttta atcacaaaga ttgtgaattt
     4561 ttcccttgtc ataaaactaa taaaccagaa gagtttaact gtctattttg ctattgtcct
     4621 ttgtatgctt taggagagaa ttgtggtgga aactttaagt atacagataa aggtatcaaa
     4681 gattgcagta gttgcatgtt acctcataaa aaagataatt acaattacat tatgggaaaa
     4741 ttccaagatt tagttaaaat cactagtaaa aagtaatttc tattggaaat agctatgcat
     4801 aaatatttat tgactactct tagcctaaat attattacat agctattttt attgtataac
     4861 ctttaatata atttgttttt gttattttat tttaaaatat ttacataatt tatacattac
     4921 aaatagtaaa atacttgaca ataaaattaa tattaaaatc atcattcctg agtgaaaaaa
     4981 aaattcctct ggcaatatat ttataactgg gtctaaattg ggattaaata tccaataatc
     5041 atttctaaat aataatttat gaaaaataat aaatgacttt tcaaaattca ataaaattgg
     5101 aactgttaat aataatggca tcattataag tgttatagat gt
//
LOCUS       DAFNNB010000188         5100 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0193, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000188 DAFNNB010000000
VERSION     DAFNNB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0193"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            36..932
                     /locus_tag="L3M60_003235"
                     /pseudo
     CDS             36..932
                     /locus_tag="L3M60_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888496.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            1334..3238
                     /locus_tag="L3M60_003236"
     CDS             1334..3238
                     /locus_tag="L3M60_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HBN6227867.1"
                     /translation="MKIKFIIVVSIIFLIVLLVGCQSTKDTEINVLATSDLHSIIPDN
                     LVSYVEEERKYDENLLMVDAGDFFDMQSGEMNKWFTGQKLLRFKDGIPEYKKIAEPRE
                     GEVPLAKKMAKLKYDAVTFGNHEFVSNDKQSLDKLVSDFKNNDIPLVSANVYEQSGEN
                     YVNPYVMKNINTDKGVVKVGILGLTIKEVGERSRFKSFEQDKKARELGEQPQYKGKLY
                     ANDLVEDANKWVKVMEKKEKPDIIVAIVHSGEEPKNPKNPGNRIKELATTVEGIDAIV
                     AGHTHEKIEQHTYKNNSGEEVIVTQPGAHGNSISKINFKLDKKNDKWFIKDKYSELKI
                     FGKEVNIITTSGLNPKFSDEMAVNLFNERDRDVQPIFIDTGDFLNTNIKEMTKWAKEW
                     QYNENKGIHKKINEWPIVYLGYDAVVLGSHEFTEDKETFEEKKSTLDYIIENFEEMKI
                     PVLSANIYDESGENYVKPYIIHNVETREGNIKVGVLGLTTTDKDEENLSSSSLSKNSK
                     TSKIPEETGKLYTNNLVKDASEWVKVMQKESPDVIVAVVHSDNNSTLKNPSSEVKKLA
                     MSVDGIDAIVVGHTKNKVEEHIYKNKSGDEVIVTQSGEDCYSKISLELRKENGKWNII
                     NKYSKAIKMK"
     gene            3603..4382
                     /locus_tag="L3M60_003237"
     CDS             3603..4382
                     /locus_tag="L3M60_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HBN6227868.1"
                     /translation="MSYKNIAIITGANSGFGKEFLKLLINEEEITEIWAVARNKARLN
                     QLVDEFGSKVKVFSKDLSKIEEVKEIGTFLSKENVCIKYLINNAGFAKFCSYNDLSID
                     ESINMIDLNISAVVALGLICIPYMQKGSHIINISSQASFQPLPYQNIYSSTKAFVRNY
                     TRALNVELKSKGINAITVCPGWMSTNLFKRGIVVTEKGTKHFPGIVSPDVVAKKALKD
                     TKKNKDISVYGINTKSSHLLAKLLPQKMMMKVWLMQQHIKE"
BASE COUNT         2091 a          527 c          886 g         1596 t
ORIGIN      
        1 caggaaccct gtgactttag tcatgggagg ttcagaagga agtaagtata gttgctactg
       61 cagatttaca ttcacatttt tctgataaat taacagtcga ccttgttaat gaacgaagca
      121 acccaattga acctgttgta gtagatgcag gtgatttctt agaccctcaa acagatgaaa
      181 tgactcaatg gtataaagag tggaaaaata taagagataa taatagtggt aaaacgatta
      241 gtagatgccc aatggtcatg gatatgaatc agggaatgta tgatgcagta gtacttggaa
      301 atcatgagtt tgtatctgaa aaagatgagt tttggtcaga tgagcgtctt ttagatgctg
      361 ttatagagga ttttgaacag gtgacaattt ctgtgttatc agcaaatata tataagcaat
      421 ctggtaaaaa ttatgttaaa ccatatatta taaaagatgt agagacgagt gaaggtaatg
      481 taaaagtagg aatattaggg cttactataa aagaagtagg taaaggtctt gaaaatgtaa
      541 atttacaaga gcaatttcaa tataagggga aattgtatgc aaatgattta gtagaagatg
      601 caaagaaatg ggtaaaaatt atgaaaaaga aaaaaccaga tataatagtt gcagttgtac
      661 attcaggaga agagcctaaa aatccaaaac atccaggaaa tagaataaag gagctagcaa
      721 ctacagtaga tggtattgat gcgattgttg ctagtcatac tcatgaaaaa atagatgaac
      781 atgaatataa aaataagtct ggagagaaag ttatagtgac acaaccaggt gagcatggta
      841 aatattattc taaaattact tttaaagtaa ataaagaaaa gggttcatgg attattaatg
      901 ataaattcag taaaacaatc gaggttgaat aactttataa aatctataga taaaataaat
      961 tatctgatat tgacataata tacaagataa agtttccaat attagtacag agcataaggt
     1021 aaaatattct aataataaga gaataaacaa gataaataaa aatatttcaa catataatat
     1081 atagtatgaa catattatac tactccttac tttataattt gaacatgtta gaataagtct
     1141 tacttgacaa ttttagtgta ttagagtaag tattattttg ccaataaaaa acattgagaa
     1201 ctatatgtag atttgcttaa aaaggaattt acatatagtt cttttcgtat aataaatagt
     1261 acaatcttat gtggtaaaga taacgcgtga tattaaattt tattatcatc caaaatctaa
     1321 ggaggagtct tttatgaaaa taaaatttat tatagtagta tcaataatat ttttaatagt
     1381 attacttgta gggtgtcaaa gtactaaaga tacagaaata aatgtactcg ctacaagtga
     1441 tttacattca attattccag ataatttagt ctcttatgta gaggaagaga gaaaatatga
     1501 tgagaatcta cttatggtag acgcaggaga tttttttgac atgcaaagtg gtgagatgaa
     1561 caagtggttt actggtcaaa aactattaag atttaaagat gggataccag agtacaaaaa
     1621 aatagccgaa ccacgtgaag gtgaagttcc attagctaaa aaaatggcaa agctgaaata
     1681 tgatgcagtt acatttggga atcatgaatt tgtatctaat gacaagcaga gccttgataa
     1741 attagtttct gattttaaaa ataacgatat accattagta tcagcaaacg tatatgaaca
     1801 atctggggaa aactatgtaa atccatatgt aatgaaaaat ataaatactg acaagggagt
     1861 tgtaaaggtt ggtatacttg gtttgactat aaaagaagta ggggaacgtt caaggtttaa
     1921 aagttttgaa caagataaaa aggctagaga acttggggag caacctcaat acaaagggaa
     1981 attatatgcg aatgatttgg tagaagatgc aaataagtgg gtaaaggtta tggaaaaaaa
     2041 agaaaagcca gatataatag ttgctattgt acattcaggt gaagagccaa aaaatcctaa
     2101 aaatccagga aatagaataa aagaacttgc aactactgta gaggggatag atgctattgt
     2161 agcaggtcat acacatgaaa aaatagaaca gcatacatat aaaaataatt ctggtgaaga
     2221 agtaatagta acacagcctg gagctcatgg taatagtata tcaaagatta attttaagtt
     2281 ggataagaag aatgataagt ggtttataaa agataaatat agtgaattaa aaatttttgg
     2341 aaaggaagta aatataatta caacctctgg tctgaatcca aaattttcag atgaaatggc
     2401 agttaatttg tttaatgaaa gagataggga tgttcaacct atatttatag atactggaga
     2461 ttttttaaat actaatataa aagaaatgac taaatgggct aaagagtggc aatacaatga
     2521 aaataaaggt atacataaaa aaataaatga atggccaatt gtatatcttg gttatgatgc
     2581 tgtagtactt ggtagtcatg aatttacaga agataaagag acttttgagg aaaaaaaatc
     2641 tactttggat tatattatag aaaactttga agaaatgaag ataccagtat tatcagctaa
     2701 tatatatgat gaatctggag aaaattatgt aaaaccttat ataattcata atgtagaaac
     2761 tagggaagga aatataaagg ttggagtatt aggtcttact actactgata aggatgaaga
     2821 aaacttaagt agctcaagct taagcaaaaa ctcaaagact tcgaaaattc ctgaagaaac
     2881 aggaaagtta tataccaaca atctagtaaa agatgcaagc gaatgggtaa aagttatgca
     2941 aaaggaaagt cctgatgtga ttgttgcagt tgtacattca gataataata gtactctaaa
     3001 aaatccaagt agtgaagtaa aaaaacttgc aatgtcagta gatggaatag atgctattgt
     3061 tgtaggtcat acgaaaaata aggtagaaga acatatttac aagaataagt ctggggatga
     3121 ggtcatagta acacaatctg gagaagattg ttattcaaaa ataagtcttg aactaagaaa
     3181 agaaaatggc aagtggaata tcataaacaa atatagtaaa gcaataaaaa tgaagtgaat
     3241 ttattgatat ataaaccatt attaacactg atttttataa tatttaacac tactttttat
     3301 actataatta acactgattc tataatatca ttaacattac ttttatacta taattaacac
     3361 tgattctata gtattattaa cactactttt tatactataa ttaacactga ttctataata
     3421 tcattaatat tacttttata ctataattaa cactgattct ataatattat taacattact
     3481 tcttatgcta tataacaatt attgaaaatg atatgaatat aacatctgaa tagagggtgt
     3541 tttatttgta aaaaataagt ttacagtttg agcgtataaa cttatggtaa aggagaataa
     3601 ttatgagtta taagaatatt gcaattataa caggtgctaa tagtggtttt ggtaaagagt
     3661 ttctaaaatt attaataaat gaagaagaaa taacagaaat atgggcagtt gcaagaaaca
     3721 aggcgagatt aaatcaatta gtagatgaat ttggaagtaa ggtaaaagtt ttttcaaaag
     3781 acttatcaaa gatagaagaa gtaaaagaaa ttggtacatt tttaagtaaa gaaaatgtat
     3841 gtataaaata ccttataaat aatgctggtt ttgcaaagtt ctgttcatac aatgatttaa
     3901 gtatagatga atctattaat atgattgatt taaatattag tgctgttgta gcattggggc
     3961 ttatatgtat tccatatatg caaaagggaa gtcatatcat aaatatttct tcacaagcat
     4021 cattccaacc attgccatac caaaatattt atagttcaac taaagcattt gtaagaaatt
     4081 atacaagagc attaaatgtt gagcttaaaa gtaaagggat aaatgcaatt actgtatgcc
     4141 caggttggat gagtacaaat ctatttaaga gaggtatagt tgtaactgaa aaaggaacaa
     4201 aacatttccc aggcatagtg tctccagacg ttgttgctaa aaaagcattg aaggacacaa
     4261 agaagaataa ggatatttct gtatatggta taaatacaaa atccagtcat ttactagcaa
     4321 aactattgcc acaaaagatg atgatgaagg tttggttaat gcaacaacat attaaggaat
     4381 aagagttttg gtaattaagt atcagttaga gattcaatct gttacaaatt atgagaattt
     4441 ttgttgagta aataataatt gtgacaaaac ggcttatcta gtgctgataa gccgtctaca
     4501 atgtatataa aatatatttt taatttcaaa gttattacac caccttctta aaatattaga
     4561 ttatataaat aatctacaat cacaactgat taacaaaatt atgagttaat aaattatatc
     4621 acatgcaaat atgaaagaat agtatgaaat ttttataaac atatattaaa tgtttctcaa
     4681 tgaaattgac tttgtatatg aactgaatga cttattcaat atggataaga catttttctt
     4741 gtaataagat atgattgtcc tataaggttt atgtaatttt gatataatat attgattaac
     4801 agcatacatg ttattggtga acatatttct atagaatgga atatataatt ttttatattt
     4861 atcattgtat cattgtctaa agaaagttaa aaagagtaga gcacaaagga attatttgta
     4921 ctttactctt ttattgtttt gttaaaaata ctaatttata ttagtgatta aaatatattt
     4981 aactagagta taaggatagt atataatctt ataattgatt tatctgacaa attaagataa
     5041 tatactaatc aaattctggt tatttaaaca taaaatcata attgatttat ctgacaaatt
//
LOCUS       DAFNNB010000189         5060 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0194, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000189 DAFNNB010000000
VERSION     DAFNNB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0194"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            351..1052
                     /locus_tag="L3M60_003238"
     CDS             351..1052
                     /locus_tag="L3M60_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6227869.1"
                     /translation="MINIAICEDNFVDRGILNDYLIRIMNENSYNISLFKSGEELLDN
                     YPDDLDILLLDILMKKINGIDTAKKIREFDSNVEIIFITSLLEYALQGYEVKAFRYIL
                     KPIEYEQLSINIIQCLDDINNKASKFVYIKCNSDIVKIDIESILYIEKISRYIFVYTE
                     DQVYKSKMNLKDIEKKLQHYKFYRCHLGYLVNVNKIDCLGSSEVSIYNHSIPVSRGKR
                     KILKEFIIDSKEKSL"
     gene            1433..2347
                     /locus_tag="L3M60_003239"
     CDS             1433..2347
                     /locus_tag="L3M60_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6227870.1"
                     /translation="MLLAYIFLIFLVFIIKFVVTKNNFIKNIHLIFFTFINIVSFLLI
                     FRGAAKDINVNFVDNIFIIIIGFTIVIFNLYLLSMMDKLLESRRIEIENKFMKEKIEI
                     EYKYIKKLQYSNENIRILKHDIKNHLICIRNIHNNKEAFDKYLDSVDIEVNKINDIYD
                     TGSIIVDAILDEKKIICDNYNIKLYVKANFTGCDFISAFDLCTIMSNLLDNAIESNLK
                     VKNSDCRNITVKANCEKYMLIIIVENNKSNKIIKSKQSIITDKKNKTIHGLGLKSVKK
                     SISKYNGDLFVEYNDENFKVKIIIPTNI"
     gene            2441..2614
                     /locus_tag="L3M60_003240"
     CDS             2441..2614
                     /locus_tag="L3M60_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227871.1"
                     /translation="MIKIKKITDKEKVVVSAQAKKPCSCAGQVLKYPPVSNSCLYDYD
                     DYNQPRWYEAQCQ"
     gene            2687..3946
                     /locus_tag="L3M60_003241"
     CDS             2687..3946
                     /locus_tag="L3M60_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HBN6227872.1"
                     /translation="MNYKFDDSVKLLKNGEYVILGNTKNGQWIKMTNYIYNIFKIGID
                     NSLDINELKKCLEDDEDRQFIQNLYENLLDMRIIYDEINNVAFQNKIASLEITNRCNL
                     KCVHCCVDADNLISEKEELSTIELKDAIKKIVEWNPMNIMLSGGEPMVRSDFFEILTY
                     LKSIYKGNIIVSTNATLINKNNVELLIKNVNKLEISLDGVNEQTCSIVRGKGVFKKVI
                     DSVNLIKSFGFDNITLSMAVGNKNEYLEEEFKDLCKNLGVGHMIRRFGPVGRGKENEH
                     LFLDNNKDEAYIPENFLKDNYDGNMGVTSCKAGIYEIFIRHNGDIYPCPSFMSDEYKI
                     TNLCDIQQISEIKDKYINKVDVFSILEKINPEIYKNCIDCNVNLFCWTCPGEIEELKD
                     SKASLFNKCSKIKNILYKRVWGNDGVC"
     gene            3940..4956
                     /locus_tag="L3M60_003242"
     CDS             3940..4956
                     /locus_tag="L3M60_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HBN6227873.1"
                     /translation="MLATFWITNECNMKCRYCYEGNNKNNKVMSKTLIDKSISYVLNQ
                     SNSLDDDELVIQIHGGEPFLEFNLIKYIISEFKSEKFNKNIIFSTTTNGMLLNNDILD
                     FIQLYIAELTISIDGAKNTHDKNRQSINGKGTHDIVIENSRKILKIKPNVRVRMTFDT
                     ETVGNLSNDIKYLISEGFRTIVPVPDMFETRWNNNHIEVLKNQIYQIKEIIGNSKDIL
                     VSLTDTNIYKYKGVCSGGKLSFNICPDGYIYPCTLAVGNKDFSIGNIYDGIDKTSRDK
                     FLSKSYLDNNDCKGCDLSNSCDGNRCKIINKLTRGSYFLPSPINCAIQNIKHEINISK
                     EIVL"
BASE COUNT         2171 a          397 c          779 g         1713 t
ORIGIN      
        1 aagtaaatct attatttatt tcatcatatt ctacaaaagt atttcataac aagttagagc
       61 aatttcatga aaatatcatc attataatga aaaaaagaaa atgttgatac agatagtatc
      121 ttttgttgtt ttttgaataa aacagaaatt ttaaagtgaa ctttaagact attcttattc
      181 agaaagtgat cttcataaaa atgtattttt ataaatttat gttgtctgta ataatttaat
      241 tatttataat taaaagttgt tatttctatg aataaattac tttatattta atcaaatgtt
      301 ataatacaat taaaatgtat taatatccaa taacagttag ggggattcta atgattaata
      361 ttgctatatg tgaagataat tttgttgata gaggtatatt aaatgactac ttaattagaa
      421 taatgaatga aaactcatat aatataagtt tattcaaaag tggagaagag ttacttgata
      481 attatccaga tgacttagat atattattat tagatattct gatgaaaaaa ataaatggaa
      541 tagatacagc aaaaaaaata agagaatttg atagtaatgt tgaaattata tttataacat
      601 cattattaga atatgcgtta caggggtatg aagtaaaagc ttttagatat atattaaaac
      661 caattgaata tgaacaactg tcaattaata tcatacaatg tttagatgat attaataata
      721 aagcaagtaa gtttgtatat ataaaatgta atagcgacat agtaaagata gatattgagt
      781 caattttata tatcgaaaaa atatcaagat atatttttgt atatactgaa gatcaagtat
      841 ataaatctaa aatgaattta aaggacatag agaaaaaatt gcaacattat aaattttata
      901 gatgtcattt aggttattta gtaaatgtaa acaaaataga ttgtttaggc tcaagtgaag
      961 taagtatata taatcatagt attccagtaa gtaggggaaa gaggaagata ttaaaagagt
     1021 ttataattga ttcgaaagaa aagagtttat aattatgtat ttgtatgaga tatggattat
     1081 tataaacttc atttcattga tagtagactt tttaattatt aataaaattt ttacttatct
     1141 tagcaagaag aaaacatcta gaaactttat gtttatcagc tttcttattg ttataattat
     1201 cttatttttt ttaaattcaa attctaataa tgaacccatg atatatattt tttttagcat
     1261 aattttgtat tatataaact atgaaactag tttagttaaa tcaataatag taccatcatt
     1321 atattggata atatatcttt tcttaattaa atatatttct tctgttttag ttattaatat
     1381 taactacaca gaacagcaag tattaaaagg tgatatattt gcattagaga tgatgctatt
     1441 agcatatatt ttcttaattt ttttagtgtt tataataaaa tttgttgtta caaaaaataa
     1501 ttttataaaa aatatacatt taatattttt tacatttata aatatagtga gttttttatt
     1561 aatatttaga ggtgcagcca aagatataaa tgtaaatttt gtagataata tattcataat
     1621 aattataggt tttacaattg tgatatttaa tttatattta ttatcaatga tggataaact
     1681 tctagaaagt agacgaattg aaatagaaaa taaatttatg aaagaaaaaa tagaaattga
     1741 atataagtat ataaaaaaac tgcaatatag caatgaaaat ataaggattt taaagcatga
     1801 tattaaaaat caccttattt gtataaggaa tatacataat aataaagaag catttgataa
     1861 atatttggat agtgtcgata ttgaagttaa taaaataaat gatatatatg acactggtag
     1921 tataattgta gatgctatat tggatgaaaa gaaaattata tgtgacaatt ataatataaa
     1981 gttatatgta aaggcaaatt ttacaggatg tgatttcatt agtgcatttg atttatgtac
     2041 aataatgtca aacctattag ataatgcaat agaatcaaat cttaaagtaa aaaattcaga
     2101 ttgtagaaat ataactgtaa aagcaaattg tgaaaaatat atgctgataa taatagtcga
     2161 aaataataag tctaataaaa taattaaatc taaacagagt ataattactg ataaaaaaaa
     2221 taaaactatt catggattag gattaaaaag tgtcaaaaag tctattagta aatataatgg
     2281 agatttattt gtggaatata atgatgaaaa ttttaaagtt aaaataataa taccaacaaa
     2341 tatatgacga gttatgcacg aaaaatgaca atctatgtac aatatgttga taaagtaaga
     2401 tgcataaact actatgtaat tacaaagggg ggtaataaat atgataaaaa taaaaaaaat
     2461 aacagataag gaaaaggttg ttgtttcagc tcaagcaaaa aaaccatgtt catgtgcagg
     2521 tcaagtatta aagtatccac ctgtaagtaa ttcttgttta tatgattatg atgactataa
     2581 tcaacctaga tggtatgagg cacaatgtca ataagtaata ctaaagccac ttttataata
     2641 taaaggtggc tttatctata aaatatgtct ttattgggag gagaatatga attataaatt
     2701 tgatgatagt gtaaaacttt taaaaaatgg agaatatgta attcttggta atacaaagaa
     2761 tggtcaatgg attaaaatga caaattatat ttataatata tttaaaatag gaatagataa
     2821 ctctttagat ataaatgaat taaaaaaatg tttagaagat gatgaagata gacaatttat
     2881 acaaaatctt tatgagaatc tattagacat gagaataata tatgatgaaa ttaataatgt
     2941 agcttttcaa aataagatag cctctcttga aataacaaat agatgtaacc ttaaatgtgt
     3001 tcattgttgt gtagatgcgg ataatcttat aagtgaaaaa gaagagctgt ctacaataga
     3061 acttaaggat gcaataaaaa aaatagttga atggaatcct atgaatatta tgttaagtgg
     3121 tggagaaccc atggtaagaa gtgatttttt tgagatacta acatacttga agtctatata
     3181 taaaggaaac ataatagttt caacaaatgc gactctaata aataaaaata atgtagagtt
     3241 attaataaaa aatgtgaata agctagaaat tagcttagat ggagtaaatg aacaaacttg
     3301 cagtatagta aggggaaaag gtgtatttaa aaaggtaatt gatagtgtta atcttataaa
     3361 gagctttggt ttcgataata taacactatc aatggctgta ggtaataaaa atgaatattt
     3421 agaagaagaa tttaaagatc tatgcaaaaa cttgggagta gggcatatga taaggcgatt
     3481 tggtccagta ggaagaggaa aagaaaatga acatttattt ttagataata acaaagatga
     3541 agcttatata cctgaaaatt ttttaaagga taattatgat ggaaatatgg gtgtaacatc
     3601 ttgtaaagca ggtatttatg aaatatttat tagacacaat ggagatatat atccttgtcc
     3661 atcatttatg tcagatgaat ataaaattac aaatttatgc gatatacaac aaataagtga
     3721 gattaaagat aaatatataa ataaagtgga tgtattttct atattagaaa aaataaatcc
     3781 agaaatttat aaaaattgta tagattgtaa tgtaaattta ttttgttgga cttgtccagg
     3841 agaaattgaa gaactaaaag atagtaaagc aagtttattt aataagtgta gtaaaataaa
     3901 aaatatttta tataaaagag tgtgggggaa tgatggagta tgttagcaac attttggatt
     3961 acaaatgaat gcaacatgaa atgcagatat tgctatgaag gtaataataa gaataataaa
     4021 gttatgtcta aaacgttgat agataaatca ataagttatg tattaaatca atctaatagt
     4081 ttggatgatg atgaacttgt aatacaaatt catggtggag aaccattttt agaatttaac
     4141 ttgataaaat acataataag tgagtttaaa agcgaaaaat ttaataaaaa tataatattt
     4201 tcaacaacta caaatggaat gctcttgaat aatgatattt tagattttat acaattatat
     4261 attgcagaat taaccataag catagacggt gctaaaaata cacatgataa aaatagacaa
     4321 agtataaatg gaaaaggtac acatgatata gttatagaaa atagtagaaa aattttaaaa
     4381 ataaaaccaa atgtaagagt aagaatgaca tttgatacag agacagtagg taatttatct
     4441 aatgatataa aatatttaat aagcgaagga tttagaacga ttgttccagt tccagatatg
     4501 tttgagacta gatggaacaa taatcatata gaggttttga aaaatcaaat ataccaaata
     4561 aaagagatta taggtaacag taaagatatt cttgtaagcc tcacagacac aaacatatat
     4621 aagtataaag gtgtttgctc tggaggaaag ttaagcttta atatatgtcc agatggatat
     4681 atatatccat gcacattagc agttggaaat aaagatttta gtataggaaa catatatgat
     4741 ggtatagata aaactagtag agacaagttt ttgagtaaat catacctaga taataacgat
     4801 tgtaaagggt gtgacttaag caattcatgt gatggaaata gatgtaagat aataaataaa
     4861 ttaactagag gttcgtattt tttaccatcg ccaataaatt gtgctataca aaatataaaa
     4921 catgagataa atataagtaa agaaattgta ttatagtttt gtagagttaa ggaggaatag
     4981 cttgaaacga attaaggttg cattaattga ctcaggtata gatattaatc ataaatattt
     5041 aaagaataat atattgtatg
//
LOCUS       DAFNNB010000190         5040 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0195, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000190 DAFNNB010000000
VERSION     DAFNNB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0195"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            248..397
                     /gene="rpmG"
                     /locus_tag="L3M60_003243"
     CDS             248..397
                     /gene="rpmG"
                     /locus_tag="L3M60_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBN6227874.1"
                     /translation="MRVKVTLACTECKQRNYNTTKNKKNNPDRIELQKYCRFCKKHTT
                     HKETK"
     gene            423..644
                     /gene="secE"
                     /locus_tag="L3M60_003244"
     CDS             423..644
                     /gene="secE"
                     /locus_tag="L3M60_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBN6227875.1"
                     /translation="MAAQKNDGAKTKKRFSLFGYLKETKQELKRVTWPTKKELFKNTG
                     IVLTVVISCTILVWGIDTILSGALALLLK"
     gene            664..1206
                     /gene="nusG"
                     /locus_tag="L3M60_003245"
     CDS             664..1206
                     /gene="nusG"
                     /locus_tag="L3M60_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination factor
                     NusG"
                     /protein_id="HBN6227876.1"
                     /translation="MSELQEASWYVVHTYSGHENKVKATIEKAVKTRGMEDCIRQVVV
                     PTEEVVETTKTGKEKTRQRKVYPSYVLVKMIITDESWYVVRNTKGVTGFVGPGSKPVP
                     LSEDEVKAMGIDTTDPKVVNSDIDFEIGDTVKVSQGPFSGQIGNIEEIDLENREVKVC
                     INAFGKRTLFVIELEGIEKI"
     gene            1254..1679
                     /gene="rplK"
                     /locus_tag="L3M60_003246"
     CDS             1254..1679
                     /gene="rplK"
                     /locus_tag="L3M60_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBN6227877.1"
                     /translation="MAKKVIGQIKLQIPAGKATPAPPVGPALGQHGVNIMGFTKEFNA
                     KTADQAGMIIPVVITVYQDRSFSFITKTPPAAVLIKKALNLKSGSGEPNKKKVAKMTS
                     AQVREIAELKMPDLNAASVEAAMSMIAGTARSMGVVIED"
     gene            1750..2448
                     /gene="rplA"
                     /locus_tag="L3M60_003247"
     CDS             1750..2448
                     /gene="rplA"
                     /locus_tag="L3M60_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBN6227878.1"
                     /translation="MAKKGKRYAGALQKVDRTKFYDASEALTLVSDIAGAKFDETVEA
                     HIKLGVDSRHADQQVRGAVVLPHGTGKTKRVLVFAKGEKAKEAEQAGADFVGAEELVQ
                     KIQGENWFDFDIVVATPDMMGVVGRLGRVLGPKGLMPNPKSGTVTFDVAKAIDEIKAG
                     KVEYRLDKTNIIHVPVGKVSFGGEKLTENFTALMDAIIKAKPAAAKGQYLRSITVASS
                     MGPGVKINPARTAE"
     misc_feature    2500..2628
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            2671..3177
                     /locus_tag="L3M60_003248"
     CDS             2671..3177
                     /locus_tag="L3M60_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBN6227879.1"
                     /translation="MRKAIEIKSEVVSEIVEKLQKSSAAVVVDYKGLTVEEVTELRKQ
                     MREAGVDYKVYKNTLVRRAAKEVGIEQFNDELLVGTNAIAFGYDDPVAPARILKGFMD
                     SHPKMKLKMGIVEGAFYDESKIVEMANIPSREVLIAKLLGSLKAPVSNFAYLIDAIAK
                     KAEGQEEA"
     gene            3235..3600
                     /gene="rplL"
                     /locus_tag="L3M60_003249"
     CDS             3235..3600
                     /gene="rplL"
                     /locus_tag="L3M60_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000159736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBN6227880.1"
                     /translation="MTIEQILEAIENMKVLELNELVKAAEEKFGVSASAPVMVAGAAA
                     GGPAAEEKTEFDVVLTDVGSSKVGVIKAVREITGLGLKEAKEVVDNAPKTVKEGASKE
                     EADQIKEKLEAAGAKVEVK"
     gene            3969..4757
                     /locus_tag="L3M60_003250"
     CDS             3969..4757
                     /locus_tag="L3M60_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBN6227881.1"
                     /translation="MEKLQGKIAVVTAATKGIGLASAEILAKNGATVYLAARSEELAN
                     EVINKISAEGGCAKFVYFNAREEETFTSMIEEVVKKEDKIDILVNNFGSTNPSLDRDL
                     LTGDTDNFFDTVNTNLKSVYLPCKAAIPHMIKNGKGSIVNISSIGSVLPDLSRIAYCV
                     SKAAINSLTQNIATQYAKDNVRCNAVLPGLIATKAALDNMSPAFIKEFLKHVPLNRIG
                     EPDDIAKAVLFYASDDSSFITGDLLEVAGGFGLPTPQFADNILG"
BASE COUNT         2027 a          581 c          955 g         1477 t
ORIGIN      
        1 ctttactaaa ctgatggatt aaaaggaata acaaaaagag gggttatgct cctctttttt
       61 taacatagaa atacaaaaac attgacacag cataggattt tgtgataact tatacaaggt
      121 aatgattata ggaagttaat aatatcttga atactgtaga tttttaataa atacatattt
      181 taaagatatt atattcacat atattaacaa tatatattat tgtattacaa agttggaggt
      241 gtatcagatg agagttaaag taactttagc atgtacagag tgtaagcaaa gaaactacaa
      301 cactactaag aataagaaaa ataatccaga cagaatagaa ttacaaaaat attgtagatt
      361 ctgtaaaaag catacaactc ataaagaaac aaaatagttt tttataagga tgtgagtatg
      421 gaatggctgc ccaaaaaaat gacggtgcaa aaactaaaaa aaggtttagt ttgtttggat
      481 acttaaaaga aactaaacaa gaattaaaaa gagttacatg gccgacaaaa aaggaactgt
      541 ttaaaaacac aggtatagtt ttaactgtag ttatttcatg cactatatta gtatggggta
      601 tagacactat attatctggt gcactagcgt tattactaaa atagacgaaa agaggtaagt
      661 taaatgtcag aattacaaga agcaagttgg tatgtagttc atacttattc gggacatgaa
      721 aataaagtta aagctacaat tgagaaagca gttaaaacaa gaggtatgga agactgcata
      781 agacaagtag ttgttccaac agaagaagta gtggaaacta ctaaaactgg aaaagaaaaa
      841 actagacaac gtaaagttta tccaagctat gttttggtta aaatgataat aactgatgaa
      901 tcttggtatg tagtaagaaa tactaaggga gttacaggtt tcgttggacc tggttcaaaa
      961 ccagtgccat taagtgaaga cgaagtcaaa gctatgggga tagatacaac agaccctaaa
     1021 gtagtaaata gcgatattga ttttgaaata ggagatacag taaaagtatc tcaaggacca
     1081 tttagtggac aaataggtaa catagaagaa attgacttag agaatagaga agtcaaagta
     1141 tgcataaatg catttggtaa aaggactcta tttgtaatag agcttgaagg tatagaaaaa
     1201 atctaatact atatttataa tattatagaa aaatctgagg aggtgcgctc aaaatggcta
     1261 aaaaagttat aggtcaaata aaattacaaa tacctgcagg aaaggctact ccagcgccac
     1321 cagttggacc agcattagga caacatggtg ttaatataat gggattcaca aaagaattta
     1381 atgcaaaaac tgcagatcaa gctggaatga taataccagt tgttataact gtatatcaag
     1441 atagatcatt cagttttata acaaaaactc caccagctgc agttttaatt aaaaaagcat
     1501 taaacttaaa atcaggttct ggagaaccaa acaagaaaaa agttgctaaa atgacttcag
     1561 ctcaagtaag agaaatagct gaattaaaga tgcctgactt aaatgctgca tcagtagaag
     1621 ctgctatgag tatgatagct ggtactgcta gaagcatggg tgttgtaatc gaagactaat
     1681 taaaagatac actttttttt ataaagtggg aggcgagaag ccgaaattaa ccacaaggag
     1741 gaaaataaaa tggctaaaaa aggtaaaaga tatgcaggtg cattacaaaa agtagataga
     1801 actaaatttt atgatgcatc agaagcatta acattagttt cagatatagc tggagctaaa
     1861 tttgatgaga cagtagaagc acacattaaa ttaggtgttg actcaagaca tgctgaccaa
     1921 caagtaagag gtgcagttgt attacctcat ggaactggta aaactaaaag agttttagtt
     1981 tttgctaaag gtgaaaaagc taaagaagct gaacaagctg gtgctgactt tgtaggagct
     2041 gaagaattag ttcaaaaaat acaaggtgaa aactggtttg actttgatat agtagttgct
     2101 actccagaca tgatgggtgt agtaggtaga ttaggtagag tattaggacc taaaggttta
     2161 atgccaaacc ctaaatcagg aacagttaca tttgatgtag ctaaagctat agatgaaata
     2221 aaagctggta aagttgaata cagattagac aaaactaaca taatacacgt tccagtagga
     2281 aaagtatcat ttggtggaga aaaattaact gaaaacttta ctgcattaat ggatgctata
     2341 ataaaagcta aaccagctgc tgctaaaggg caatatttaa gaagtataac agtagcttct
     2401 tcaatgggac ctggagttaa aataaaccca gcaagaacag ctgaataata aaactgttga
     2461 cacaggttga caaaaatgtt atcatatcac ttgtcaatgt aaaaaagaat agatttgccg
     2521 tagatagtgg gtgcgaaagc ttaatatccc gccgaggttt tagataaatt gagattacta
     2581 aagcttttga tgtctacttt tggcaacaaa agctttttat aatgttgcgg taaatactaa
     2641 gcctatttgg tgaaggaggt gcacatctat atgagaaaag ctatagaaat taaatcagaa
     2701 gttgtttctg agatagttga aaagttacaa aaatcttctg ctgctgttgt tgtagattac
     2761 aaaggattaa cagttgaaga agtaactgag ttaagaaaac aaatgagaga agctggtgta
     2821 gattacaaag tatataaaaa tactttagtt agaagagcag ctaaagaagt tggtatagag
     2881 caatttaatg atgaattatt agttggaact aatgccatag catttggata tgatgatcca
     2941 gttgctccag ctagaatatt aaaaggattc atggattctc atccaaaaat gaaattaaag
     3001 atgggtattg tggaaggtgc attttatgat gagtctaaga ttgttgaaat ggctaacata
     3061 ccatcaagag aagttcttat cgctaaatta cttggtagct taaaagctcc agtatcaaac
     3121 ttcgcatact taatagatgc aatagcgaaa aaggcagaag gtcaagaaga agcttaattg
     3181 cttaactaaa aaattataaa tattaatttg aaataaaatt cggaggtgct aaaaatgaca
     3241 atagaacaaa tattagaagc tatagaaaat atgaaagttt tagaattaaa tgaattagtt
     3301 aaagctgctg aagaaaaatt cggtgtatca gcttcagctc cagtaatggt agctggtgca
     3361 gctgctggtg gaccagctgc tgaagaaaaa actgagtttg atgtagtatt aactgacgtt
     3421 ggttcttcaa aagttggagt tataaaagca gttagagaaa taacaggatt aggattaaaa
     3481 gaagctaaag aagtagttga caatgctcct aagacagtta aagaaggagc ttctaaagaa
     3541 gaagctgatc aaataaaaga aaaattagaa gctgctggag ctaaagtaga agttaagtag
     3601 tttctttaaa aagataccca ttgggtatct tttttattta taaaaaatta taaaggaagt
     3661 atttttataa aataaaaaaa aattatataa tagtactaaa ttaagggtat catatatgat
     3721 gtaatggtat tttaatttaa tattattata aaattaaaat ataaacaatt gttgtaatgt
     3781 atatatataa agtatataat taaattgaaa tgtaaaaaat tacatattat tttaaaaaaa
     3841 tacacgatag ttttataaat aaaatgtaat aaataattaa ttaacaaata tgaattttaa
     3901 aataagttaa acatttagaa aaatcgaaat ttgcaggatt taaagacata tttaaggagg
     3961 agttaataat ggaaaaatta caaggaaaaa ttgcagtagt tactgcggca acaaaaggta
     4021 ttggattagc atcagcagag atattagcaa aaaatggagc aactgtgtat ttagcagctc
     4081 gttcagaaga attagctaat gaagttataa ataaaataag tgcagaaggt ggctgtgcta
     4141 aatttgtcta ctttaatgca cgtgaagaag aaacttttac ctcaatgata gaagaggtag
     4201 ttaaaaaaga agacaagata gatatacttg taaataactt tggttcaaca aacccttctc
     4261 ttgatagaga ccttttgact ggtgatacag ataatttttt tgatacagta aatactaatt
     4321 taaagagtgt atatttacca tgtaaagcag caattcctca catgataaag aacgggaagg
     4381 gaagcatagt aaatatatcc agtataggct cagttttgcc tgatttatct agaatagctt
     4441 actgtgtatc aaaagcagca atcaattcat taactcaaaa tatagctaca caatatgcca
     4501 aagataatgt tagatgtaat gctgtacttc caggtcttat tgcaactaag gctgcattag
     4561 ataatatgtc tccagcattc ataaaagaat ttttaaaaca tgttccttta aatcgtatag
     4621 gagaaccaga tgatatagca aaagcagttt tattctatgc tagtgatgat tcatcattta
     4681 taacaggaga tttgcttgag gttgcaggtg gatttggttt acctactcca caatttgcag
     4741 ataatatatt aggataatta aattactaat atgtaatgaa ggctttatgt attagttata
     4801 gtatataagg atatttttat aaagaccata gatataattt atattggaaa agtttgcggt
     4861 tcaaaattcc aagttaaatt tatttatggt ttttattttg catattctaa taaattgtat
     4921 atatttgtat aaatgtaatt atttacaatc attttaatat agaattataa ttaaataggg
     4981 gggatggtat ttgtataaaa ttttatatag aaaattagtc ctataaatga aaaatatgga
//
LOCUS       DAFNNB010000191         4996 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0196, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000191 DAFNNB010000000
VERSION     DAFNNB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4996
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0196"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            52..1119
                     /locus_tag="L3M60_003251"
     CDS             52..1119
                     /locus_tag="L3M60_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HBN6227882.1"
                     /translation="MKNKLIYICIMSLLLFVVGCSNNPNKESTIKKDSKNNQSQVKEE
                     KREPVKKDITISFAGDVTMGNYKGSSYYGSFDHEFKRQGEDYNYFLKNVKPVFKKDDL
                     TVVNLEGPLTTASNAKEKKFAFKGKPGYVNILKSGSVEAVSVANNHSEDYFEEGMKDT
                     QFILDENKINYFGLGKDAVVDVKGIKVGLLGYNGLSTEYNEKNLKQMEDDIKSLKKKS
                     DVVVVYFHWGIELDYYPDKKQKDFAHYAIDKGADLVVGSHPHVIQGIEKYKNKYIAYS
                     LGNFCFGGNKNPSDTDSYIYQQTFTFSDNKLTSIKEPHIIPTSITSSSSRNNYQPKVL
                     DGSEKDRVLNKLEKISKDLNQ"
     gene            1289..3190
                     /gene="cooS"
                     /locus_tag="L3M60_003252"
     CDS             1289..3190
                     /gene="cooS"
                     /locus_tag="L3M60_003252"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBN6227883.1"
                     /translation="MACNTCKMCESADKKLESFVASKDVETAFHRTEDQKVKCGFGLQ
                     GVCCRLCSNGPCRVTPKSPRGICGADADTIVARNFLRAVASGAACYLHVVENTAKNLK
                     NVGITKGVIKGEKTLNQLAEMFGIESDEKYDKCIKVADKVINDLYKSRDDKMELVEKI
                     AYAPRVKKWKELGIMPGGAKSEVFDAIVKSSTNLNSDPVDMLVNCLNLGISTGLYGLT
                     LTNLLNDVMLGEPVIRMAPVGFNVIDPDYINIMITGHQHSTFANFQDRLKDEDVIKLA
                     QSVGAKGFRLVGCTCVGQDLQLRGEHYQEVFAGHAGNNFTSEAVLSTGAIDIVLSEFN
                     CTIPGLEPIADKYKVKMVCLDDVAKKANAEYIGLDRSKLDELSNTLIEKALESYKERR
                     GSIEIDIPKDHGFEQSLTGVSEKNLKAFLGDSWKPLINLIAEGKIKGVAAVVGCSNMT
                     AGGHDVNTVELTKELIKKDIIVLSAGCSTGGLENVGLMSPGAEELAGENLKEVCKTLG
                     IPPVLNFGPCLAIGRLEIVATELAAELGIDLPQLPLVLSAPQWLEEQALADGAFGLAL
                     GLPLHLALPPFITGGKLVTEVLTEKLKDLTGGHVIVNPDPKSSANQLEEIIIDRRKNL
                     GLKDVECNA"
     gene            3183..3656
                     /locus_tag="L3M60_003253"
     CDS             3183..3656
                     /locus_tag="L3M60_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBN6227884.1"
                     /translation="MHRIFINKDLCTGCKSCVLACMLKHNKDYDMYTLDLENIDNDSR
                     GHIELDSKHNNPVPILCRHCDEPECVLACMSGAMHKDSESGIVSYDEEKCGSCYMCVM
                     SCPYGLLKPDDRSKQNILKCDLCKDEEYPRCVANCPSGAIELQKEENDELCSVRG"
     gene            3631..4857
                     /locus_tag="L3M60_003254"
     CDS             3631..4857
                     /locus_tag="L3M60_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBN6227885.1"
                     /translation="MNYVVLGASAAGINAVKTLRELDKDSNIVVISKDENIYSRCMLH
                     HVISEHRTLKQINFVDEDFMEQNNVNWIAGKTVKGIDVNKKVVQTEDITVNYDKLLIA
                     TGASSAIPPIKNLREGNFVYSVRNIDDIYKIKEKAQNSKNVVIIGAGLVGIDALVGLF
                     KYEQLNISVAFMEKYILDRQLDEYTASVYENKFKEKGVKFYPSASIQEIILDDSKNVK
                     GVAFSNGEILDADMVIVATGVKPNADFLDETGIEYDRGIIIDDMCQTTQKDIYAAGDV
                     VGKNAIWPLAVKQGIVAAYNMAGKDKKIEDEFAFKNSMNFMDIPTISIGMNTPVDDSY
                     KVLTRHGLNDYKKFIFKDNVIYGAVIQGDISYVGVLTYLIKNKVEIYDLENRIFDVCY
                     ADFFKIKENGEFCYSV"
BASE COUNT         1990 a          515 c          977 g         1514 t
ORIGIN      
        1 ctaaaaaagt atattttatt ttaaaattta ttaatacaaa ggagatttac tatgaagaat
       61 aaattaattt acatatgtat catgagtcta cttctatttg tagtaggatg ttctaataat
      121 ccaaacaaag aaagtactat taaaaaagac tctaagaata accaaagcca agttaaagaa
      181 gaaaaaagag aacctgtgaa aaaagatata actataagtt tcgctggtga tgtaactatg
      241 ggaaactata aaggctcgtc atattatggt agttttgacc atgaatttaa aagacaagga
      301 gaagattata attattttct taaaaatgta aaacctgtat ttaaaaaaga cgacttaaca
      361 gtggttaatt tagaaggtcc acttactaca gcgtcaaatg caaaagaaaa aaaatttgca
      421 tttaaaggaa agcctggata tgtaaatatt ttaaaatctg gaagtgtaga ggcagtgagt
      481 gttgcaaata atcattcaga agattatttt gaagaaggaa tgaaagatac acagtttata
      541 ttggatgaga ataaaataaa ttattttgga ttagggaaag atgctgttgt agatgtcaaa
      601 ggaattaaag tgggtctatt agggtataat ggcttatcaa ctgagtataa tgaaaaaaac
      661 ttaaaacaaa tggaagatga tattaagtcc ttaaaaaaga aatctgatgt tgttgtagtg
      721 tactttcatt gggggataga attagattat tatccagata aaaaacaaaa agattttgct
      781 cattatgcaa tagacaaggg agctgattta gttgtggggt ctcacccaca tgtgattcaa
      841 ggaatagaaa agtacaaaaa taagtatata gcttatagtt tagggaattt ttgttttgga
      901 gggaacaaga atccttctga tacagatagt tatatatatc aacaaacttt tactttttca
      961 gataataaat taacttcaat caaagagcca catataatac caacatcaat aacatctagt
     1021 agttctagaa ataattatca accaaaagta ttggatggaa gtgagaaaga tagagttttg
     1081 aataaactcg aaaaaataag taaagattta aatcaataaa atataaaaaa aaataaatat
     1141 atatatttat tttaataatt tatattctgt ttaggatatg ggaaatcgtg gtattaaata
     1201 agtagatttt aatagttaaa ttatagtcaa ttataataaa gctatttaat ctactttttt
     1261 aatataaaaa tataaggggg aatttgtaat ggcttgtaac acttgtaaaa tgtgcgaaag
     1321 tgcagacaag aaattagaaa gttttgtagc ttcaaaggat gttgaaacag catttcatag
     1381 aacagaagat cagaaagtta agtgtggttt tggacttcag ggggtttgtt gtagactctg
     1441 ttcaaatgga ccatgtagag taactcctaa atcaccaaga ggtatatgtg gagcagatgc
     1501 agatactata gtagcaagaa actttttaag agcagtagca tcaggagcag cttgttattt
     1561 acatgtggta gaaaatacag ctaagaattt aaaaaatgtt ggaataacta aaggtgttat
     1621 taaaggagaa aaaacattaa atcaattagc agagatgttt gggatagaaa gtgatgaaaa
     1681 gtatgataaa tgtataaagg ttgcagacaa agttatcaac gatttgtata aatcgagaga
     1741 tgataaaatg gaattagtag aaaaaatagc ttatgcacct agagtgaaaa agtggaaaga
     1801 gttaggtata atgcctggtg gtgcaaagtc agaagttttt gatgcaatag taaaatcatc
     1861 aacaaattta aatagtgacc cagtagacat gctagttaat tgtttgaact taggtatatc
     1921 aacaggatta tatggtctta cactaactaa tcttttaaat gatgttatgt tgggagaacc
     1981 agttataaga atggctcctg taggatttaa tgtaatagac ccagactaca taaatataat
     2041 gataacaggt catcaacatt caacatttgc aaatttccaa gatagattaa aagatgaaga
     2101 tgttataaaa ttagctcaat ctgttggtgc aaaaggattt aggctagtag gatgtacatg
     2161 cgttggtcaa gatttacaat taagaggaga acattatcaa gaagtatttg ctggacatgc
     2221 aggtaacaat tttacaagtg aggctgtttt atcaactgga gcaatagata tagttctttc
     2281 agaatttaac tgtactatac cagggcttga accaatagca gataaatata aagtaaaaat
     2341 ggtttgttta gatgatgttg ctaaaaaagc gaatgcagaa tacataggtt tagacagaag
     2401 taaattagat gaacttagta acactttaat agaaaaagca ttggagtctt ataaggagag
     2461 aagaggaagt attgaaatag atataccaaa agaccatgga tttgaacaat ctttgactgg
     2521 tgtaagtgaa aagaacttga aagcgttttt aggagattca tggaaacctc ttattaattt
     2581 aattgcagaa ggaaaaatta aaggtgttgc agctgttgtg ggatgttcaa atatgacagc
     2641 aggaggacat gatgtaaata ctgttgagct tactaaagaa ttgattaaaa aagatataat
     2701 agtactatct gcaggatgtt ctactggagg attagaaaat gttggtctta tgtcacctgg
     2761 agcagaagaa ttggctgggg aaaacttaaa agaagtatgt aagactttag gaataccacc
     2821 agtattaaac tttggaccat gtctagctat aggaagacta gaaatagtag caacagaatt
     2881 agcagctgag cttggaattg acttaccaca attaccacta gtgctttcag ctccacaatg
     2941 gttggaagaa caagcattag ctgatggagc ttttggtctt gctcttggtt tacctcttca
     3001 tttagcttta cctccattta taacaggagg aaaattagta acagaagtat taacagaaaa
     3061 attaaaggac ttaacaggag gacatgttat agtgaatcca gaccctaaat cttcagctaa
     3121 tcaattagaa gaaataataa tagacagaag aaaaaattta gggttaaagg atgtggaatg
     3181 taatgcatag aatttttata aataaagatt tatgtacagg atgtaagagc tgtgtactag
     3241 catgtatgct aaaacacaat aaagattatg acatgtacac tcttgattta gaaaatatag
     3301 ataatgatag tagagggcat atagaattag atagtaaaca taataatcca gttccaattc
     3361 tttgtagaca ctgtgatgaa cctgaatgtg tattagcatg tatgagtggt gcaatgcata
     3421 aagatagtga aagtggaatc gtttcatatg atgaagagaa atgtggttca tgttatatgt
     3481 gtgtaatgtc ttgtccatat ggattattaa agccagatga tagaagcaag caaaacatac
     3541 ttaaatgtga tttatgtaaa gatgaggagt atcctagatg tgtagctaat tgtccaagtg
     3601 gagcaattga attacagaag gaggaaaatg atgaattatg tagtgttagg ggctagtgcg
     3661 gctggtataa atgcagtaaa aacattaaga gaattagata aagattcaaa tatagtagta
     3721 atttctaaag atgaaaacat atactcaaga tgtatgttgc atcatgttat atctgaacac
     3781 agaactttaa aacaaataaa ttttgttgat gaagatttta tggagcaaaa taatgtaaat
     3841 tggattgcag gcaagactgt taaaggtata gacgtaaata aaaaagtagt tcagacagaa
     3901 gacattactg taaattatga taaactttta atagctacag gtgcatcttc tgctattcct
     3961 cctataaaaa atttaagaga aggaaatttt gtatattctg tgagaaacat agatgacatt
     4021 tataaaataa aagaaaaagc acaaaattct aaaaatgtag ttataatagg tgctggtcta
     4081 gttggtatag atgcactagt gggattattt aaatatgagc agttgaatat ttctgtagct
     4141 tttatggaaa agtacatttt agatagacaa ttggatgaat atacagcatc agtgtatgaa
     4201 aataagttca aagaaaaagg tgtaaaattt tatccaagtg catcaataca agaaatcata
     4261 ttggatgaca gtaaaaatgt taaaggtgtt gcattttcaa atggagaaat tttagatgca
     4321 gacatggtaa ttgtagctac aggggttaaa ccaaatgctg actttttaga tgaaacagga
     4381 atagaatatg atagaggtat aataatagat gatatgtgtc aaacaacaca aaaagacata
     4441 tatgcagcag gagatgttgt aggtaaaaat gctatttggc ctctagcagt aaagcaagga
     4501 attgtagctg catataatat ggctggaaaa gataagaaga tagaagatga atttgctttt
     4561 aaaaatagta tgaatttcat ggatatacca actatttcta taggaatgaa tacacctgta
     4621 gatgatagct ataaagtatt aaccagacat ggacttaatg attataaaaa gtttattttt
     4681 aaagataatg taatctatgg agcagttata caaggagata tttcatatgt aggtgtatta
     4741 acttatttaa taaaaaataa agttgaaata tatgatttag aaaacaggat atttgatgta
     4801 tgttatgcag acttttttaa gataaaagaa aatggagaat tttgttatag cgtataatat
     4861 atatggagta aggtttataa atacagtaaa tcaataaata cagtaaatca ataaatagag
     4921 ttattctaaa ataagaaaaa tgagaaatct tatttagaaa tagctctatt tttgtaatta
     4981 agtttaaatt atattt
//
LOCUS       DAFNNB010000192         4957 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0197, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000192 DAFNNB010000000
VERSION     DAFNNB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4957
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0197"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            190..1497
                     /locus_tag="L3M60_003255"
     CDS             190..1497
                     /locus_tag="L3M60_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227886.1"
                     /translation="MKEIADAITSEYLSYGINEDISIYEGRFCIYLDKKYRCNGKIYY
                     KMTPPISISFKAEIGCVEEIDSEDDNLALDYDNAILEVHGYKIISITINTLSEFSVEG
                     YINDDCIKSKNSYVEYVDFNIINLDKIPGKLIKYNDKVYAGRIEFDINDYVITIDKRY
                     DYRKELKLELKSKSGAIITHIGRIRRKDGRIFRTNNTINLLDRISTALSFMCGRYVGF
                     CLAKGYRSGNEVYRIWNENQISPFRYVPTWSDTLSNYHNMEKYISLMCKKLEDFYYGS
                     AIKSVVDWYIESLGSATMENNIISVQIALETLSYAILVEQSKVLTDEAFDCNLASRNI
                     RLLLDTCKIPYGKHELNIFDNIIKNKFDDGVDLVIYLRNSIVHPSRKTHRAVLEVEDI
                     WNIISIGTRYIELVLLFILGYRGEYSNRLVERCYGEVEVVPWN"
     misc_binding    1583..1849
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1975..2778
                     /locus_tag="L3M60_003256"
     CDS             1975..2778
                     /locus_tag="L3M60_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBN6227887.1"
                     /translation="MKNILKKVGIFTIMLGLLGGVVGCSKPDNEKDKDASKESKKEVV
                     VGFDNTFVPMGFLDEKGNTVGFDVDLAKETFKRLGMEVKFQPIDWSMKETELNDSKTV
                     DVLWNGYSITDERKKIVSYTEPYLQNKQIIVTLSDSKINSKADLKDKEVGTQQGSTAL
                     DAVEKDKDFMNSLKGGAPVLYDTYDKALRDLEIGRTSAVVGDEVLIRYYMGQKGEDKY
                     KVLKDDFGLEDYVVATSKENPELCEKINETLKEMKKDGTFDKIYDKWFK"
     gene            2816..3427
                     /locus_tag="L3M60_003257"
     CDS             2816..3427
                     /locus_tag="L3M60_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBN6227888.1"
                     /translation="MLQGLEIVIAMFCITLIISIPLGIGVAFLRLSKNKLVSGITQCY
                     ILIMRGTPLLLQMIVIFYGLPLLGIVFDRFTAGVVAFFLNYAAYFAEIFRGGIQSIDR
                     GQYEASKVLGFDKFTMYKRIIFPQVFKRILAPISNEVITLVKDTSLVYILGLNDILRI
                     SQIAMNREASLLPLFEAGAIYLIFVAILTKGFELLEKKYSYYR"
     gene            3458..4129
                     /locus_tag="L3M60_003258"
     CDS             3458..4129
                     /locus_tag="L3M60_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBN6227889.1"
                     /translation="MLKIKNLNKSFKKNRVLKDISFELEEGQIGVLLGKSGAGKTTIL
                     RCINGLEEFDSGEIIIDNEVIKNKRDMAKIRGKIGMVFQNFNLFPHMTVLENIIESPV
                     NVFKVPRKEAEERARELLRLVDLEDKLNSYPFELSGGQQQRVAIARSCALMPKVLCFD
                     EPTSALDIDTIQRVVNIMNRLKDKGMTILIITHDVVFSNNVADKIISIKDGIVENVQI
                     KEKIV"
     gene            4424..4786
                     /locus_tag="L3M60_003259"
     CDS             4424..4786
                     /locus_tag="L3M60_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HBN6227890.1"
                     /translation="MIKLYGYTKCSTVKKAKNWLKENNLEFEDIDMVQNPPNKEELKS
                     IYKTSGYDIKKFFNTSGVKYRELGLKDIVKTESDDKLLEILVSDGMLIKRPLLFDGKN
                     VLLGFKEDVWKSTLLKED"
BASE COUNT         1990 a          444 c          882 g         1641 t
ORIGIN      
        1 aattacaagt tagttattta atttttcgaa taactaaatc taaacggaat acataatgta
       61 ctaaataaat gatgatacta aaatgatact atttttatag aaatttattg aggtatataa
      121 ttaatgaaaa taatatatta aattatataa tatattgagt tttacataaa agcgaaggtg
      181 ggaataaaaa tgaaagaaat tgctgatgct ataacatcag agtatttaag ctatggtatc
      241 aatgaagata ttagtatcta tgaaggtaga ttttgcatat atttagataa aaaatataga
      301 tgtaatggga aaatttatta taaaatgaca cctccaatat ctataagttt taaagcagag
      361 ataggttgtg ttgaagagat agacagtgaa gatgacaatt tagctttgga ttatgataat
      421 gctattcttg aagttcatgg atataaaatt atttctataa caataaatac tttaagtgaa
      481 tttagtgtag agggttatat caatgatgac tgcataaaat ctaagaactc ctatgttgaa
      541 tatgtagatt ttaatataat aaacttagat aaaatacctg gaaaactcat taaatacaat
      601 gataaagtat acgcaggtag aattgaattt gatataaatg attatgttat aactatagac
      661 aagaggtatg attatagaaa agaattaaaa ttagaattaa aatctaaatc tggagcaata
      721 ataactcata taggtagaat tagaagaaaa gatggtagaa tttttagaac aaataataca
      781 ataaatcttt tagatagaat atcaacagct ttgagtttta tgtgtggaag atatgtaggg
      841 ttttgtttgg ccaaagggta tagaagtggt aatgaagttt atagaatatg gaatgaaaat
      901 caaatatctc cttttagata tgtaccaaca tggtcagata cactttcaaa ttatcataat
      961 atggaaaaat atataagtct tatgtgtaaa aagctagaag atttttatta tggttccgct
     1021 ataaagagtg ttgttgattg gtatatagaa tctcttggaa gtgctactat ggaaaacaat
     1081 ataatatctg tgcaaattgc acttgaaact ttgtcatatg caattttagt agaacaaagt
     1141 aaggtactta cagatgaagc ttttgactgt aatttggcat ctagaaatat aagactctta
     1201 cttgatacct gtaagatacc atatggtaaa catgaattaa atatatttga taatataata
     1261 aaaaataaat ttgatgatgg agtggattta gttatttatt taagaaatag catagttcat
     1321 ccaagtagaa agactcatag agctgtatta gaagttgagg atatttggaa tataatttca
     1381 ataggaacga gatatattga gcttgtttta ctttttattt taggttatag aggagaatat
     1441 tcaaatagat tagtagaaag gtgttatggt gaagtggaag ttgtaccttg gaactagatt
     1501 tttatattta ttttaaaata tatattgaca tatattacct tatgctataa aatcaatatc
     1561 ataataacaa ttcagaataa cattaaatgc taagaaaaga aatagtaaaa gtagttaatc
     1621 ttaaacagag agtttccgat tggtgagagg agcaatagat tttactttga acacatcttt
     1681 gagcaggact ctgaaagagt agaaatactt tattaggagc tttccggaat ccttgcaccc
     1741 gttatagtgc tagagtataa tgatttgttt tattgtactt aaagaggtta gtgtcgtgag
     1801 gcattaatga acagaggtgg aaccacgaga gttaactccc gtcctctagc agaagctaga
     1861 ggcgggagtt ttttaatata attttttaag cgcgcagaga attatattgc aagagataca
     1921 ctttttaaaa aataatcaaa atattttaaa aattaaaagg cgagggagaa tattatgaaa
     1981 aatatattaa aaaaagtagg aatatttaca attatgttag gtttattagg tggagttgta
     2041 ggttgttcaa agccagataa tgaaaaagat aaggatgctt caaaggaatc taaaaaagaa
     2101 gtagttgtag gatttgataa tacatttgta ccaatgggat ttctagatga aaagggaaat
     2161 acagtaggat ttgatgtgga tttagctaaa gaaacattta agagacttgg aatggaagtt
     2221 aagtttcagc ctatagattg gtcaatgaag gaaactgaat taaatgattc aaaaactgta
     2281 gatgtattat ggaatggata ttctataaca gacgaaagaa agaaaatagt atcatataca
     2341 gaaccatatt tacaaaataa gcagataata gttactctaa gtgattcaaa gataaattca
     2401 aaagctgatt taaaagataa agaagttgga acacaacaag gttcaacagc acttgatgca
     2461 gtggaaaagg ataaagattt tatgaatagt ttaaaaggtg gagcaccagt tctttatgat
     2521 acttatgata aagcacttag agatttagag ataggtagaa ctagtgcagt tgttggtgat
     2581 gaagttttaa ttagatacta tatgggacaa aaaggtgaag acaaatacaa agtgttaaaa
     2641 gatgattttg gattagaaga ttatgttgta gcaacttcta aagaaaatcc agaactttgt
     2701 gagaaaataa atgaaacatt aaaagaaatg aaaaaagatg gaacttttga caaaatttat
     2761 gataagtggt ttaaatagaa gataagttag ctgttaagaa gggggcacta aaggcatgtt
     2821 acaaggttta gagatagtta tagcaatgtt ttgtataaca ttgataatat caattccatt
     2881 ggggattgga gttgcatttt taagattatc aaagaataaa ttagtaagtg gtattacaca
     2941 atgttatata ttgataatga gaggtacacc tttacttttg caaatgatag tcatatttta
     3001 cggattacct cttttaggaa ttgtatttga cagatttaca gctggagtag tagctttctt
     3061 cttaaattat gcagcatatt ttgcagaaat atttagaggt ggaatacagt caatcgatag
     3121 aggacaatat gaagcttcaa aagtactagg atttgataaa tttactatgt ataagagaat
     3181 aatctttcca caggttttta aaagaatact agcacctatt tcaaatgaag taatcacatt
     3241 agtaaaagat acatctttag tatatatatt aggattaaat gatattttaa gaatatctca
     3301 aatagcaatg aacagggaag ctagtctgtt accattattt gaggcaggag cgatatatct
     3361 tatatttgta gctatactta ctaagggatt tgaattactt gaaaaaaaat attcttatta
     3421 tagataaagt tacaatatta taggaggagg tttttatatg ttgaaaatca aaaacttaaa
     3481 taaatcattt aaaaaaaata gagtacttaa agatatttct tttgagttag aagaaggtca
     3541 gattggagtt ttacttggta agtctggtgc aggaaaaacc acaatattga gatgtataaa
     3601 tggtcttgaa gaatttgata gtggagagat tataatagat aatgaggtaa taaaaaataa
     3661 gagagatatg gcaaaaatac gagggaaaat aggtatggtt tttcaaaatt tcaacttatt
     3721 tcctcatatg acagtccttg aaaatataat tgaatcccca gttaatgttt ttaaagttcc
     3781 tagaaaagag gctgaagaga gggctagaga acttttaaga ttagttgatt tagaagacaa
     3841 attaaattct tatccatttg aattgtcagg aggacagcaa caaagagttg ctatagctag
     3901 gtcatgtgca ttgatgccaa aagttctttg ttttgatgaa cctacttctg cacttgatat
     3961 tgatactatt caaagagtag tcaatataat gaacagactt aaagataaag gtatgacaat
     4021 attaataatt acacatgatg ttgtcttttc aaataatgtt gcagataaaa ttataagtat
     4081 aaaagatggt atagtagaaa atgttcaaat aaaagaaaaa attgtttaga gttttataaa
     4141 tataaactag ggtagaattt tataatataa ataatgggca agtaaaatca aatatactat
     4201 gcccttttgt tgtctcaaaa tttaatctta tattttatgt ttaagttaca tttaatttat
     4261 aataaaaata aaaggactat ttaaatatat aatgtaaata aatgtttaat ttgaaacatg
     4321 ataatttttt atgttgaaag cttataatca ttatgatata aattggttat agtatacaaa
     4381 ttatgggtat aacaataaaa aattagaaag gaatttttat aaaatgatta aattatatgg
     4441 atatacaaaa tgttctactg ttaaaaaagc aaaaaattgg cttaaggaaa ataatttaga
     4501 atttgaagat atagatatgg ttcaaaatcc tcctaataaa gaggagttaa aatcaattta
     4561 taagaccagt ggatatgata taaagaaatt ttttaataca agtggtgtga agtatagaga
     4621 attgggtctt aaagatattg taaaaactga atctgatgat aaacttttag aaatattggt
     4681 aagtgatggt atgcttatta aaagaccatt actttttgat ggtaaaaatg tattattagg
     4741 ctttaaagaa gacgtatgga aaagtacttt gctaaaagaa gattagaagg atataggtgt
     4801 gttgattaaa attattaatt aatacaccta taaatttatg tattaaatct aattcttata
     4861 aatattaatt agcaaattca aatcaaaaaa attcatatca acaaacatat tacttcttgt
     4921 tacaaatgat atcatgtatt ttatttaaaa tagtata
//
LOCUS       DAFNNB010000193         4951 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0198, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000193 DAFNNB010000000
VERSION     DAFNNB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4951
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0198"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(155..1609)
                     /locus_tag="L3M60_003260"
     CDS             complement(155..1609)
                     /locus_tag="L3M60_003260"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBN6227891.1"
                     /translation="MKLNDIIQGLDIINVKGELNIDINNVQYDSRKVTKGTLFICIKG
                     FVSDGHKYIKDAIEKGASAFLVEEDVEIKGCTFIKVKDTRKDMAKVADNFYNHPSQKF
                     NVIGVTGTNGKTSITTILNEILTLNKNKVGLIGTIKIFDGEKDIVSNSTTPESIDLQY
                     HFNNMLDNGCDYCAMEVSSHSLALNRVDETDFKLGIFTNLTPDHLDFHKDLEDYRKAK
                     EKLFFKTTMANIINIDDEGGKKIYENIKGINVPCYTYGVDTNADFMARDIKSDSDGVS
                     YRLITPSYEEVIFIPVPGMFTVYNTLAVIAACYVLGIPKPIYKEGLRLSNGVSGRFET
                     VPNDKGISVIVDYAHTPDALENVLKTTQQFAEGKIISVFGCGGDRDTEKRPLMGAIGQ
                     KYSDLCIITSDNPRTEEPEAIIKDILEGIDKKKENYHVVVDREQAISEAISMAKKDDV
                     VIITGKGHETYQIIGKVKHHFDDKEVANECLSKM"
     gene            1773..2642
                     /locus_tag="L3M60_003261"
     CDS             1773..2642
                     /locus_tag="L3M60_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227892.1"
                     /translation="MDNKIGYLNDNFKIFNIRDKKNIQFEYHNHDFNKIIIFINGNVT
                     YFVEGIPYKLKPWDILFISNNEIHKPVIDSNVFYERIAIWISPEFMKKNSDATSDLEK
                     CFKISTENKCNRLRLNMHDIESIKSFIEQINISESNTEFGNEILRNALFIQLMIFLNR
                     IFLKNENIESTEDVFYDETIKKVLDYINSNIDDKLSIDSISSEFFISKYYLMRKFKSH
                     TGTSIHNYIVQKRLILAKSLITNGHLMSEVCNKCGFNDYSSFVRAFKKNYGVSPKNYI
                     NSKNPLDITLLDE"
     gene            complement(2856..4928)
                     /locus_tag="L3M60_003262"
     CDS             complement(2856..4928)
                     /locus_tag="L3M60_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HBN6227893.1"
                     /translation="MKKVFGVLQKVGKSLMLPVALLPAAGILLGVSNALAGDALLAHF
                     PALANPTFQLIISIMESSGQIIFNNLPLIFAVGVAVGLTEGEGVAALAAIVGFLILNT
                     SMGVMAGVTEDMIDNSMYASIVGIPTIQTGVFGGIIIGIVAAYLYKKFYKIELPQYLG
                     FFAGKRFVPIVTSLVAIAIGIILSFIWPPIQEGLLSFSKNVIEANQTLAAFMEGSLER
                     LLIPFGLHHVFHNPFWYQFGEYVNKAGELVMGDQKIFFAQLKDGVNFTAGTFMTGAFP
                     FMMFGLPAAALAMVHEAKPEKKKFVAGIMASAALTSFLTGITEPIEFAFLFVAPVLFA
                     IHCLFSGLSYAVMQLLGVKIGFTFSGGLIDFLLFGVLPNRTPWYLAILVGIGFAVLYY
                     SVFRFMIRKFNLKTPGREDDSETNVEVIDMSKNELASEILVALGNKENLKSLDACITR
                     LRLIVNDINKVDKEKLKSLGAAGVMVIGNNVQVIFGPKSDSIKSDIKDIIEGKTKIES
                     AVIKNNDKNKMKKNMEVANMENALVENIYSLTNGEILDIEEVPDSVFSSKLMGDGFAI
                     KSNDGLIYSPVDGTIGVIFPTKHAIIIKSKKSGVEILIHLGIETVNLEGNGFEVFVNV
                     GDEVKAGEKLVKMDLEYIEKNGLSTISPVVFTNLEQNQKLSIKKGITTAKEENRVIIL
                     RDDIEKIG"
BASE COUNT         1676 a          836 c          560 g         1879 t
ORIGIN      
        1 tataaacttt attacaacaa taaattaatt aatgtagata ttagggttta tattattgca
       61 ttatctattt acaatcattt acatataaaa aatgtaatat caaagtaaaa cttgatatta
      121 catttttgtt cttgtatttt gtttactttt tatgctacat tttacttaaa cactcattgg
      181 caacttcttt atcatcaaag tgatgcttaa ctttacctat aatttgataa gtttcatgcc
      241 ccttacctgt tataattaca acatcatcct tttttgccat acttatagct tctgatatag
      301 cttgttctct atcaacaaca acatggtaat tttctttttt cttgtcaatt ccttcaagta
      361 tatcttttat tattgcttct ggttcttcag ttcttgggtt atcagaagtt ataatgcata
      421 aatcagaata tttttgtcca attgctccca tcaaaggtct cttttcagta tctctatctc
      481 caccacatcc aaatacagaa attatttttc cttctgcaaa ttgttgagtt gtttttagta
      541 cattttcaag tgcatcagga gtatgagcat agtctacaat tacacttata cctttatcat
      601 ttggaactgt ttcaaacctt ccggatactc cattagaaag tctaagacct tctttatata
      661 ttggtttggg tatgccaagt acatagcaag cagctataac tgctaaagta ttatatactg
      721 taaacattcc tggaactggt ataaatatta cttcttcata tgaaggtgtt attaatctat
      781 aagatactcc atccgaatct gattttatat ctctagccat aaaatcagca ttagtatcaa
      841 ccccataagt gtaacaaggt acatttatac cttttatatt ttcatatatc ttttttccac
      901 cttcatcatc tatgtttatt atatttgcca tagtagtttt aaaaaataat ttttcttttg
      961 cttttctata atcctctaaa tctttatgaa aatctaaatg gtctggtgtt aaatttgtaa
     1021 atatgcctag tttaaaatct gtttcatcaa ctctatttaa tgccaatgaa tgtgatgaaa
     1081 cttccatcgc acagtagtca catccattat ccaacatatt attaaaatga tattgtaaat
     1141 ctatactttc tggagttgtt gaattggaaa caatgtcttt ttctccgtca aaaattttta
     1201 tagtacctat aagcccaact ttattcttat ttaaagttaa tatctcattt agtatggtag
     1261 ttatactagt ttttccatta gtacctgtaa ctcctataac gttaaatttt tgagatggat
     1321 gattgtaaaa attatcagct acttttgcca tatcttttct tgtatctttt acctttataa
     1381 atgtacatcc ttttatttct acatcttctt caacaagaaa ggcacttgct cccttttcaa
     1441 tagcatcttt tatatactta tgaccatctg acacgaatcc ttttatacat ataaataatg
     1501 taccttttgt aacctttctt gaatcatatt gtacattatt aatatctata tttaattcac
     1561 ccttgacatt gattatatcc aacccctgta taatatcatt taatttcata aatgctttcc
     1621 tccctttaaa ctctttctgc taaaattaca ctacatataa tattatatta agaatttttt
     1681 actatgtgaa tggttaaaaa tgcttgaaac attgcaaaaa atgctctata attttctata
     1741 atataactga agaaaggtct tctataaaaa ctatggataa taaaatcggg tacttaaatg
     1801 ataattttaa aatatttaat attagagata aaaaaaatat acaatttgaa taccataatc
     1861 atgattttaa taagataatt atatttataa atggaaatgt aacttatttt gttgagggta
     1921 ttccttataa actaaagcct tgggatatat tatttatcag caataatgaa atccataaac
     1981 cagttataga ttcaaatgtc ttttatgaga gaattgcaat atggattagc ccagaattta
     2041 tgaaaaaaaa tagtgatgct actagcgact tagaaaagtg ttttaaaata tctacagaga
     2101 ataaatgtaa tagattgcgt ttaaatatgc atgatattga atctataaag agttttattg
     2161 agcaaataaa tatttctgaa tccaatactg aatttggaaa tgaaatattg agaaatgcat
     2221 tatttataca acttatgata ttcttaaaca gaattttcct aaaaaacgaa aatatagaat
     2281 caacagaaga cgttttttat gatgaaacta ttaagaaagt attagactat ataaattcaa
     2341 acatagatga taaactttca atagatagta tatcgtctga attttttata agcaagtatt
     2401 atcttatgag aaaatttaaa tctcatactg gaacgtctat acacaactat atagttcaaa
     2461 aaagattgat tttggcaaag tcattaataa ccaatggtca ccttatgagt gaagtttgta
     2521 ataaatgtgg attcaacgat tattctagct ttgtaagggc gtttaaaaaa aactatggtg
     2581 tatctcctaa aaattatata aacagtaaaa accctttaga cattactctt ttagatgaat
     2641 agataattta tgtaaatttt attttgataa aaacttgagg tgttagtata tttatcttac
     2701 tagtacctca agttttttaa ttttttaaaa tatatcaaaa tataatgata tatatttcta
     2761 ttacttaaaa tatatactaa ctaaattgga tgttaactta acctatttat gtgttcatta
     2821 aatctcatgt aacttttagt tatactatca tatagctatc ctattttttc aatatcgtct
     2881 cttaatataa ttactctatt ctcctctttt gctgtagtta ttcctttttt aatgcttaat
     2941 ttctgatttt gttctaaatt cgtaaatact acagggctta ttgttgatag accatttttt
     3001 tcaatatatt ctaagtccat tttaactaat ttttctcctg cttttacttc atctcctaca
     3061 ttcacaaata cttcaaatcc atttccttct aagtttacag tttctatccc taaatgtata
     3121 agtatttcta ctccactttt tttggatttt attattattg catgcttagt tggaaatata
     3181 actccaatag ttccatcaac tggggaataa atcaacccat cattagattt aattgcaaat
     3241 ccatccccca tcaatttact tgaaaataca gaatctggca cttcttctat atctaatatc
     3301 tctccatttg ttaatgaata aatattttct actaaagcat tttccatatt ggcaacttcc
     3361 atattttttt tcattttatt tttatcgtta tttttaataa cagcactttc aatctttgtt
     3421 tttccctcta ttatatcttt tatatcagac tttattgaat ccgattttgg tccaaatata
     3481 acttgcacat tgttgccaat aaccataaca cctgctgctc caagtgattt caatttttct
     3541 ttatctactt tatttatatc atttacaatc aatctaagtc ttgttataca cgcatctaga
     3601 ctctttaagt tttctttatt tcctaaagca accaaaatct ctgatgctaa ttcattctta
     3661 gacatatcaa tgacttcaac attagtttct gaatcatctt ctcttcctgg tgtctttaaa
     3721 ttaaactttc ttatcataaa tctaaataca ctatagtata atactgcaaa accaatacct
     3781 accaaaatag ccaagtacca tggagttcta ttaggaagaa caccaaataa taagaaatct
     3841 attaatcctc ctgaaaatgt gaatccaatt ttaactccta ataattgcat aactgcgtat
     3901 gataaaccag aaaataaaca atgtattgca aataatactg gtgcaacaaa taaaaacgca
     3961 aactctattg gctcagttat tcccgttaaa aaagatgtta atgctgctga tgccattata
     4021 cctgctacaa attttttctt ttctggtttc gcttcatgca ccatagcaag agctgctgct
     4081 ggaagaccaa acatcataaa tggaaatgca cctgtcataa aagtacctgc tgtgaaattt
     4141 acgccatctt taagttgagc aaagaatatc ttttggtcac ccattacaag ctcaccagct
     4201 ttatttacat attctccaaa ttgataccaa aatggattat ggaaaacatg atgcaaacca
     4261 aatggtatta ataatctttc taaagaacct tccataaatg ctgctagtgt ttgatttgct
     4321 tctataacat tttttgagaa actaagtaat ccctcttgta ttggaggcca aataaaagac
     4381 aatataattc ctattgctat agcaactaat gaagtaacga ttggtacaaa tcttttacct
     4441 gcaaaaaaac ctagatactg aggtaattca attttataaa actttttata aagataagca
     4501 gccactatac ctataataat accaccaaat actcctgttt gaattgttgg tattccaaca
     4561 atacttgcat acatagaatt atctatcata tcttctgtaa cacctgccat aacacccata
     4621 gatgtattaa gaattaaaaa acctactata gctgccaatg ctgcaacacc ttctccttcg
     4681 gtcaaaccaa cagctacacc aactgcaaat ataagtggta agttattaaa tataatttga
     4741 cctgaacttt ccattatact tattataagt tggaatgtag ggttcgctaa agctggaaaa
     4801 tgagccaaca acgcatctcc tgctaaagca ttactcacgc caagtaatat acctgctgca
     4861 ggaagtaatg caactggtag cattagagac tttcctactt tttgtaaaac accaaatact
     4921 tttttcattt acttttctct ccttattatt t
//
LOCUS       DAFNNB010000194         4876 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0199, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000194 DAFNNB010000000
VERSION     DAFNNB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0199"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..927
                     /locus_tag="L3M60_003263"
     CDS             <1..927
                     /locus_tag="L3M60_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6227894.1"
                     /translation="ISSLCKKIKLGINQSPIMAQDIKIKYPFAFELANIAKKTIEKDL
                     KIEINEDEVANIALHIGGAVERASYNNKDKVFKTIIVCTSGIGTSMLIKAKLENIFKE
                     KLEILKIIPSYLVEYIKVIDVDFVISTVPIEVGDVPVINISPILSDKEIRLIEKYIET
                     GNIYLDLDIKSLFDSELFFTDLVFKRKEEVIDFMASKLVQKGYIDEDMKKSYFEREKI
                     ATTEIGNMVTIPHGAIGKVFDNKIAIGILKKPISWEISDVRLIIMLALDKDKILDYEL
                     IFSKIYKRVDSIAKVISICENSSFEKFVNLFN"
     gene            1094..2011
                     /gene="pfkB"
                     /locus_tag="L3M60_003264"
     CDS             1094..2011
                     /gene="pfkB"
                     /locus_tag="L3M60_003264"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBN6227895.1"
                     /translation="MIYTVTLNPSIDYIVKLNELKTGSTNRVNEEYVYPGGKGINVSR
                     ILKELGNDNISLGFISGFTGEYIIRTLEEKNLKTDFIKIKNGFSRINVKIKESEETEI
                     NGQGPNIDDEDIDILYKKLDKLNQDDILILAGSIPSTLDEKLYENIMARLEKKNIKVV
                     VDATKNLLLNVLKYKPFLIKPNNDELEELFGVKLNSIEDMVKYARRLKEMGAINVLVS
                     MGKDGALLITEEDEVLISDVPKGKVKNSVGAGDSMVAGFISGYLNTGKYDYALKLGAA
                     SGSATAFSYDLAKREYIDKLVNEISVKQF"
     gene            2048..3967
                     /locus_tag="L3M60_003265"
     CDS             2048..3967
                     /locus_tag="L3M60_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBN6227896.1"
                     /translation="MKIIDLLDEKSIKLNLNSKTKSEAIEELVDLVANSGNLNDKENY
                     KKAIFAREEMSTTGIGEGVAIPHAKNSSVTKACIAAAVSKEGIDYESFDGSLSNLFFM
                     IAAPDGANNTHLEVLSRLSTILMDEDFRNKLINSNSEKEFKEIIDKKEREKFSEEYQD
                     EKVAEKNIIATKDNNTNKYPQVLAVTACPTGIAHTFMAAESLNKMAENKGVSIKVETN
                     GSAGVKNKLTKDEIENATCIIVAADKNVEMARFNGKKVIKTKVADGIHKAEELIDKAV
                     NGDAPIYHGGDGSHNESNEESESGFRKVYKHLMNGVSNMLPFVIGGGILIAIAFLLDD
                     YAINPSNFGSNTPIAAFFKGIGDKAFGFMLPVLAGYIAYSISDRPAFVVGFVGGALAG
                     DGGSGFLGALLAGFIAGYLVEGLKKIFSVLPASLEGIKPVLLYPLLGTLLMGIIMTFL
                     VIPPVTAINNAMVGFLNGLGGTSKIVLGLVLGGMMAVDMGGPVNKAAYVFGVASLESG
                     QFEIMAAVMAGGMVPPLAIALATTFFKNRFTKEERDSGKVNYVMGLSFVTEGAIPFAA
                     GDPLHVIPACVGGSAVAGALSMLFNAALRAPHGGVFVIPVVTHPFAYILAIAVGALVG
                     MMLLALLKKPLNQEV"
     gene            4464..>4876
                     /locus_tag="L3M60_003266"
     CDS             4464..>4876
                     /locus_tag="L3M60_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6227897.1"
                     /translation="MIFTIIFYKKGRLCLLNLQSKWVNFLKPTYVSDEIKRKKLQVII
                     FFSITYGLSYSLGLLAICFNHLIDSEILAQFMMILPLSSVSIAKFYTEGRNNDKYNFY
                     SSVILFFIIYFFIFIIESLKLISINQFQLMYLMLI"
BASE COUNT         1926 a          487 c          861 g         1602 t
ORIGIN      
        1 attagtagtt tatgtaaaaa aataaagcta ggtataaatc aatctcctat aatggctcaa
       61 gatataaaaa taaaatatcc atttgctttt gaactagcta atatagctaa aaagacaata
      121 gaaaaagatt tgaagataga aattaatgaa gatgaagttg caaacatagc tctacatata
      181 ggaggagcag ttgaaagagc atcttataat aataaagata aggttttcaa aactataata
      241 gtctgtactt caggtatagg tacatcaatg ttaataaaag ctaaattaga gaatatattt
      301 aaggaaaaat tagaaatatt aaagattatt ccttcatact tagttgaata tataaaagtt
      361 atagatgttg attttgtaat atctactgtt cctattgaag taggagatgt accagtaata
      421 aatatatctc cgattttaag tgataaagaa attagattaa ttgaaaaata tatagaaaca
      481 ggaaatatat atctagattt agatataaaa agcctgtttg atagtgaatt attttttact
      541 gatttggtgt ttaaaaggaa agaagaagtt atagatttta tggcaagtaa acttgtacaa
      601 aagggatata tagatgagga tatgaaaaaa tcttactttg agagagaaaa aatagcaaca
      661 acagaaatag ggaatatggt aaccatacca catggtgcta ttggaaaagt ttttgataat
      721 aaaatagcaa ttggtatatt aaagaaacct atttcttggg aaatatcaga tgttcgttta
      781 ataataatgt tagctcttga taaagacaaa atattagatt atgaattaat cttttcaaaa
      841 atatataaaa gagtagattc catagcaaaa gttataagta tatgcgaaaa tagcagtttt
      901 gaaaaatttg taaatttatt taattagatg ggtggtattg tatatacatc tgtaaatttg
      961 gataatataa aactaaataa aataaaaacg ataaatttct cttttaatta agagaaattt
     1021 tttgactttc aaggatatta atataaagtt aagatataaa cataaggtaa ataaataaaa
     1081 aagaggtggc aatatgattt atacagtgac attaaatcca tcaatagatt atatagttaa
     1141 gctaaatgaa ctaaagacag gaagtacaaa tcgtgtaaat gaagaatacg tttatccagg
     1201 aggaaaaggt ataaatgtat ctcgtatact taaagaactt ggaaatgata atatatcttt
     1261 aggttttata agtggtttta ctggagaata tataataaga acattggaag aaaaaaattt
     1321 gaagacagat tttataaaga taaaaaatgg tttttcaaga ataaatgtaa aaataaaaga
     1381 gtcagaggaa actgagatta atggacaagg tccaaatata gatgatgaag atattgatat
     1441 tttatacaaa aaacttgata agctaaatca agatgatatt ttaatattgg caggaagtat
     1501 accatctact ttagatgaaa aactatacga aaatataatg gctagattag aaaaaaagaa
     1561 tataaaagtt gtagtagatg caactaaaaa tcttcttctt aatgttttaa aatataaacc
     1621 ttttctaatt aagcctaata acgacgaatt agaagaacta tttggtgtta aattaaattc
     1681 aattgaagac atggtaaaat atgctagaag gttaaaggaa atgggggcta taaatgtctt
     1741 ggtatcaatg ggtaaagatg gagcattgtt gattacagag gaagatgaag tgcttataag
     1801 tgatgttcct aaaggaaaag taaaaaattc tgtaggagca ggtgattcaa tggtagcagg
     1861 atttatatct gggtatttga atactggtaa atatgattat gcccttaaat tgggagcagc
     1921 ttcaggtagc gcaactgcct tttcatatga cttagccaaa agagaataca ttgataaatt
     1981 ggtaaatgaa atatctgtaa agcagtttta aaatattaaa aaatatatag aaaggtaatt
     2041 aatatttatg aaaattatag atttattaga tgaaaagtca attaagttaa atttaaattc
     2101 aaaaaccaaa tcagaagcaa tagaagaatt agtagatttg gtcgcaaata gtggaaattt
     2161 aaatgataaa gaaaactaca aaaaagcaat ttttgctcgt gaagaaatga gtacaacagg
     2221 aattggagaa ggtgtagcca tacctcatgc taaaaattct tcagttacta aagcttgtat
     2281 agcagcagca gtgtcaaaag aaggtataga ttatgaatcg tttgacggaa gtttatcaaa
     2341 tctatttttt atgatagctg caccagacgg agctaacaat actcatcttg aagtattatc
     2401 aagattatct acaatactta tggatgaaga ctttagaaat aaacttataa actcaaactc
     2461 tgaaaaagaa tttaaggaaa taatagataa aaaggaacga gaaaaattca gtgaagaata
     2521 tcaagatgag aaagttgctg aaaaaaatat aatagctact aaagataata atactaataa
     2581 gtatcctcag gtattagcag taacagcatg tccaactgga atagcgcata catttatggc
     2641 agcagaaagt ttaaataaaa tggccgaaaa taaaggtgta tcaataaaag tagaaactaa
     2701 tggttcagca ggtgttaaaa ataagcttac aaaagatgaa atagagaatg caacatgtat
     2761 aatagtagca gcagataaaa atgttgagat ggctagattt aatggcaaaa aagtcataaa
     2821 aactaaggtg gctgatggca tacacaaagc ggaagagctt atagacaagg ctgtaaatgg
     2881 agatgctcca atttaccatg gaggagatgg aagtcacaac gaatcaaatg aagaaagtga
     2941 aagtggattt agaaaagtat acaaacactt aatgaatggt gtatctaata tgttaccatt
     3001 tgttattggt ggaggtatac ttatagcgat agccttttta cttgatgatt atgctattaa
     3061 cccttcaaac tttggttcaa atacaccgat agcagcattt tttaaaggaa taggagataa
     3121 agcttttgga tttatgttac ccgtacttgc tggatatata gcatactcta tatctgatag
     3181 accagcgttt gttgtaggat ttgtaggagg tgctctagca ggtgatggtg gttcaggctt
     3241 tttaggtgca ctacttgcag gttttatagc aggttattta gtagaagggc ttaagaaaat
     3301 atttagtgta ctaccagctt cattggaagg aataaaacca gtattgttat accctttact
     3361 tggaacactt ttgatgggaa ttataatgac atttttagta atacctccag ttacagcaat
     3421 taataatgca atggttggat ttttaaatgg tttaggtgga acttcaaaaa tagttttagg
     3481 attagtatta ggtggaatga tggctgtgga tatgggtgga cctgtaaata aagcagcata
     3541 tgtatttgga gttgcatcac ttgaatcagg gcagtttgaa ataatggcag cagtaatggc
     3601 tggtggtatg gttccgccac ttgctatagc tcttgcaact acatttttta agaatagatt
     3661 tactaaagaa gaaagagatt ctggtaaagt taattatgta atgggattat catttgttac
     3721 tgaaggagct ataccttttg cagctggaga cccacttcat gttataccag cttgtgttgg
     3781 aggttcagca gtggcaggag cacttagtat gttgttcaac gcagctcttc gtgcacctca
     3841 tggaggagta tttgttatac cagtggttac ccatccattt gcatatatat tagctatagc
     3901 tgtaggtgct ttggttggaa tgatgctttt agcattactt aaaaaaccat taaatcaaga
     3961 agtatagcaa ttaaaggata ttcaagaact taaaatatga tgttttataa aaattaatat
     4021 attgctgaat tattttaaaa taaggatttt atgaggctgt tatataacga tttgtaattg
     4081 ttatcttgac agtcttttat ttttatataa gtaaaataag tgtgttttgc tttatttgta
     4141 tttaaattgt ttttaagtta gaatataaag aaaattatat attattttaa tattcactcg
     4201 aactagaaaa taatatttgt tgtatttttt tgtatataaa aatatattgt aatattaaaa
     4261 aaataatagt aaactgattg cataaaaatg atttttatat aaattttaaa cagaagaata
     4321 aatttctaac gaaattggat atttcttctg taaatacata ttcaaaaaaa gaattataag
     4381 aatttagtca gcagtcgttt aagtaataag gactttataa atatatagaa atataatgat
     4441 atgagtataa gtagaaaaat tttatgattt ttacaattat tttttataaa aaagggaggt
     4501 tatgtttgtt gaatttacaa tcaaaatggg taaacttttt aaagccaaca tatgtcagtg
     4561 atgaaataaa aagaaaaaaa ctacaagtta taatattttt ttctataact tatggtttat
     4621 cttattcact aggtttatta gcaatatgtt ttaatcattt aatagattca gaaattttag
     4681 ctcaatttat gatgatatta ccattatctt cagtttctat tgcaaaattt tacacagagg
     4741 gaagaaataa tgataaatat aatttttatt catcagtaat attatttttt attatttact
     4801 tttttatatt tattattgaa tcattaaagt taataagtat taatcaattt caattaatgt
     4861 atcttatgtt aatttt
//
LOCUS       DAFNNB010000195         4641 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0200, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000195 DAFNNB010000000
VERSION     DAFNNB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0200"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(440..2827)
                     /gene="cadA"
                     /locus_tag="L3M60_003267"
     CDS             complement(440..2827)
                     /gene="cadA"
                     /locus_tag="L3M60_003267"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HBN6227898.1"
                     /translation="MEANNLIKKEFILGGLNCAHCAEEINDKVSKLQEVKSSNLNFIN
                     KKLTVNIKESFNEDATIEKIIDIIDSTEPGLDIQINSKENASNKTSIKKELILGGLNC
                     AHCAEEINNKVSKLKEVESSNLNFVNKKLTINISNNFEEDDVINKIKEIINSTEPGLD
                     IQVWSNDKVKERTTEKSGVANDTNKKELISLIIGVLVYIFGIYQTATGYESQFSNIVF
                     IVAYVIIGGDVVLRAIRNISKGRVFDENFLMALATVGALAIGELSEAVGVMLFYKVGE
                     YLQGVAVGKSRKSITSLMQIRPDYANLKVNSEVKVVSPEEVKVGDIIVVKPGEKVPLD
                     GVVVDGVSMLDTSALTGESVLREVEKGDEILSGVINKNALLSIEVTKSFGESTVSKIL
                     DLVENSSIKKSKTENFISKFSRYYTPIVVIAALLIAFIPPLVIPGEVFSDWLYRGLIF
                     LVVSCPCALVLSIPLSFFSGIGFASKNGILIKGSNYLEALRSVDTVVFDKTGTLTKGV
                     FNVTKLNPEGISEEELLEYAAIAEVNSNHPIAKSILSYYNKKIDLDTIDSYEEIAAYG
                     IRVKHNGKFILAGNEKLMKKENISYSSAKEVGTVVYIAVDKVYRGYIVISDEVKEDSK
                     NAIKSLKAIGVKEVVMLTGDNEKVAKNIARELELDTVYSNLLPNEKVDRLEELYEGRT
                     EREKIAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVLMTDEPSKISKAIEIA
                     NKTNKIVWQNIIFALGVKMIVMILGAGGVATMWEAIFADVGVSLIAVVNAMRAMR"
     gene            complement(2850..3215)
                     /locus_tag="L3M60_003268"
     CDS             complement(2850..3215)
                     /locus_tag="L3M60_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6227899.1"
                     /translation="MREEINDCNCNIIHEEIVTEAKSTMPDEEMLYDLAELFKVFGDT
                     TRVKILYALFANEMCVCDIASLLNMTHSAISHQLRVLKQARLVKFRREGKTVYYSLDD
                     SHISQIFDCGLNHIRETYK"
     gene            3415..4305
                     /locus_tag="L3M60_003269"
     CDS             3415..4305
                     /locus_tag="L3M60_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227900.1"
                     /translation="MSNKKKKDLDTSYMPYLDPANYADYITNEEIPQGELRNDPQISN
                     GYTKVPNNQNINNLYPNNINNYPKSSNSPNNLPNTNNTSGNMNYNTSATNNMPNNMNS
                     NMGTPNNMSNNMNSNMGIPNNMPNNMNSNMGIPNNMPNNMNSNMGIPNNMPNNMNSNM
                     GIPNNMPNNMNSNMGIPNNMPNNMNSNMGIPNNMYGNMNNNMYGNTGMPNNMNSNMGY
                     NMNNTMGIPNFSCNQNMPPNVLMMIPGVICHNTMQGMPVVMPSTMPPNIYPTPYGSSN
                     MSMQGIPQATNIEEFDEEEM"
BASE COUNT         1582 a          794 c          492 g         1773 t
ORIGIN      
        1 cgtcaacact tttttaaatt atttttttaa atttttcact ttattatatt acataattta
       61 ttgaatggca gttatattgt tgatttagag tatcttagca cacacatttt attgtaaaaa
      121 ttataactat taatataata tatatgcaat cgtaagtatt ataattttta tgagtctagt
      181 ttaaacttaa tataaattta tcaataaatc ctctatatct aaattttaaa attacaatac
      241 aaaagttata tactaaataa atgcattaaa taaacataat gaatacaaat ttataaatga
      301 atatagattt atatatacaa aagtactcca ataaaaaggt atcaataaag ttaaaagaaa
      361 cttattgata cctttcatca ttgtatactt caatatataa ttataagttt tttgaatagt
      421 atagatttac atttattcct tatctcatag ctctcatagc atttactact gctataagtg
      481 acacacctac atcagcaaat atagcttccc acatagtggc tactcctcca gctcctaata
      541 tcataactat catttttact cctaaagcaa atattatgtt ttgccacact attttattag
      601 tcttgttagc tatttcaatt gctttagata tcttgctagg ctcatctgtc ataagcacta
      661 catctgcagc ttcaatagca gcatctgaac ctaaacctcc catagctata ccaacatcag
      721 cacgagctaa tactggagca tcatttattc catcacctac aaatgctatt ttttctcttt
      781 cagttcttcc ttcatataac tcttctaatc tatccacctt ttcattagga agtaaatttg
      841 agtaaactgt atctaattct aattctcgag ctatattttt tgctactttc tcattatctc
      901 cagttaacat tacaacttct tttactccta ttgcttttaa gctttttata gcatttttac
      961 tatcttcttt cacttcatca gatataacta tataacctct atatacttta tcaacagcaa
     1021 tatagactac tgtccctact tctttggctg atgaataaga tatattttct tttttcataa
     1081 gtttctcatt acctgctaat ataaacttgc cattatgctt tactctaatt ccataagctg
     1141 ctatttcctc atagctgtct attgtatcta aatcaatttt tttattatag taacttaata
     1201 tagactttgc tattggatga tttgaattta cctccgcaat agctgcatat tctaataact
     1261 cttcttctga tataccttca ggatttaact tagttacatt gaacacacct ttagtaagtg
     1321 ttcctgtttt atcaaatact actgtgtcta cacttcttaa agcttctaaa tagttacttc
     1381 ctttaataag gataccattt ttagatgcaa acccaattcc actaaagaaa ctaagtggta
     1441 ttgataaaac taatgcacaa gggcaagaaa cgactaaaaa tatcaatcct ctatataacc
     1501 aatcactaaa tacctcacct gggattacaa gtggtggtat aaatgctatt agtaaagcag
     1561 ctattactac tatcggagta tagtatctgg aaaacttaga tataaagttt tctgtctttg
     1621 attttttaat gctagaattt tcaacaaggt caagaatctt agaaactgtt gattctccaa
     1681 aactctttgt aacttcaata ctcaataaag catttttatt tataacacct gataaaattt
     1741 catctccttt ttcaacttct ctcagcactg actctcctgt aagtgcagaa gtatctagca
     1801 tagaaactcc atccactact acaccatcta atggtacttt ttcaccaggt tttactacta
     1861 ttatatcgcc tacctttact tcttctggtg acaccacctt aacttcagaa ttaactttta
     1921 gatttgcata atctggtctt atttgcataa gtgaagttat agattttcta gatttaccaa
     1981 ctgcaacacc ttgtaaatac tctcctactt tgtagaaaag cataactcct actgcttctg
     2041 ataattctcc tattgctaat gctcctactg ttgctagagc catcaaaaag ttctcatcaa
     2101 atactcttcc ttttgatata tttcttattg ctcttaaaac tacatcacca cctattatca
     2161 cataggcaac tataaatact atattactaa actgactttc ataacctgta gccgtttgat
     2221 atataccaaa tatatataca agaactccaa ttataagtga aattaattct ttcttatttg
     2281 tatcatttgc aactcctgat ttttcagtag ttctctcttt tactttgtca ttagaccata
     2341 cttgtatatc aagtcctggc tctgttgaat ttattatttc ttttattttg tttataacat
     2401 catcctcttc aaagttattg cttatattta ttgtaagttt tttatttaca aagtttaaat
     2461 tagaagattc aacttctttt aatttagaaa ctttgttatt aatctcctct gcacaatgtg
     2521 cacagtttaa tcctcctaat ataagctctt ttttaattga tgttttatta gatgcatttt
     2581 ctttagaatt tatttgtata tcaagtcctg gctctgttga atctattata tctattatct
     2641 tttctatagt agcatcctca ttaaagcttt cctttatatt tactgtaagt tttttattta
     2701 taaaatttaa gttggaagat ttaacctctt gtagtttaga aactttatca ttaatctctt
     2761 ctgcacagtg agcacaattt aatcctccta gtataaattc ttttttaatt aaattatttg
     2821 cttccatata tattcactcc tctcatttat catttataag tctctctaat atgatttaat
     2881 ccacaatcaa atatctgact tatatgacta tcatctaatg aataatacac tgtttttcct
     2941 tctcttctaa attttactaa cctagcctgc tttaatactc taagttgatg agatattgca
     3001 gagtgagtca tatttaataa actcgctata tcgcatacac acatttcatt tgcaaataat
     3061 gcatataata tctttactct agtagtatcc ccaaatactt taaataattc tgctaaatca
     3121 tataacatct cttcatctgg catagttgat ttagcctctg ttactatttc ttcatgtatt
     3181 atattacaat tacaatcatt tatctcttct ctcatttttt aatcctccgt tattttaata
     3241 tatgaataac tgttcatata ttcattatat gctatcattt ctaaatttgc aacaataatt
     3301 tttattaatt taatacaatt agaatgcaca tattataaac ttttttaata tatatattat
     3361 tagaataagt ttttgtaaac ttttttatag ttttctagga ggtgcaagtt taatatgagt
     3421 aacaagaaaa aaaaggattt agatactagt tacatgcctt acttagaccc agcaaactat
     3481 gcagattata ttacaaatga agaaattccc caaggtgaat tgagaaatga ccctcaaatt
     3541 tctaatggtt atacaaaagt acctaataac cagaacataa ataacctata tcctaataac
     3601 ataaataatt atcctaaaag tagtaattct ccaaacaact tgcctaacac aaataataca
     3661 tctggaaata tgaattacaa tacatctgct acaaataata tgcctaataa catgaatagt
     3721 aatatgggta cgcctaacaa tatgtctaat aacatgaata gtaatatggg tattcctaac
     3781 aatatgccta ataacatgaa tagtaatatg ggtattccta acaatatgcc taataacatg
     3841 aatagtaata tgggtattcc taacaatatg cctaataata tgaatagtaa tatgggtatt
     3901 cctaacaata tgcctaataa tatgaatagt aatatgggta ttcctaacaa tatgcctaat
     3961 aatatgaata gtaatatggg tattcctaac aatatgtatg gcaatatgaa taataatatg
     4021 tatggtaata caggtatgcc taataacatg aacagtaata tggggtataa tatgaataat
     4081 actatgggta ttcctaactt ctcatgtaat caaaatatgc ctcctaatgt acttatgatg
     4141 ataccaggtg ttatatgcca taatacaatg caaggtatgc ctgtagttat gcctagtact
     4201 atgcctccta atatatatcc tactccttat ggttctagta atatgtctat gcaaggtata
     4261 ccacaggcta ctaatattga ggaatttgat gaggaagaaa tgtaatatat atttttttaa
     4321 gcaaactgta taaaacatta ataattgacc ataataataa atgtattcct cataatattc
     4381 ccccaatatt attataggta tagaaagttg actgaatata cgcttattcg gtcaacaaat
     4441 attaacataa tctaataaga taaatataga aaaagtcaga taaaattaaa ttttatctga
     4501 ctttttcatt taataaactc tttcttttgt ttgtagttaa gaatggtatg atactcatat
     4561 tttagactaa tattagcaaa caacataaag aaaattataa attttttcta ataaactgta
     4621 taaaatttta ataattgacc a
//
LOCUS       DAFNNB010000196         4610 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0201, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000196 DAFNNB010000000
VERSION     DAFNNB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0201"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            318..3152
                     /locus_tag="L3M60_003270"
     CDS             318..3152
                     /locus_tag="L3M60_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6227901.1"
                     /translation="MLRKDKVLLSLHKLCENMTLKDLQSDKIGFTTIEIANHSSLDRS
                     NTSKELNNLYNEKKVLKIPGKPILFLHASIIENLIGRTLNSNEFSINNCNELLKSSKN
                     DTEDIFSTLLGYDSSLKLPIEKAKSAILYPPKGLNTLLIGPTGVGKSTFAETMYKYAV
                     ESKIFSSESKFVVFNCAEYAENANLLLSNLFGYVKGSFTGANKDKFGIVAQADGGILF
                     LDEIHRLPPEGQEMLFLLMDKGIYRRLGESDIVHKANVFIVCATTEDIHSSLLGTFLR
                     RIPVTINLPALQDRSLKERLSLIKYFFIIESKYTNATIRVHKDVIKSLLLYDCFGNIG
                     QLKSDIQLMSARAFLNYKTGLKDNIEIDLSLVPDYIYIGLLKLNENRSNLNKLSEIDY
                     MLFYEFNGNNLEVEYTKELSKYNIDTSNNGFKYSTEIPYLENDIRSMFINYMEKYSTK
                     LLSISSVTHSDYTSNEIFKVINPKIYYAVEHSLKEAEKISRNKYTKQTYIALSMHISA
                     LIENINQDTYNPKYNLYNDKIFLDKDLNIAKIVLSIIENDLQTSLPKDEVKFISMFLN
                     STYLEPNNQSESIGVIILAHGNSTASSMCDVANSLLGTNHCNAIDMPLDVKVSTILDK
                     AIELVKECDEGKGVLLLTDMGSLIAFGELISEKTKIKTRSIEMVSTPIVLEAVRKSTL
                     PEMTLDELANSLSSLNPYIGRAAIDSTEISYPEKSREYVIITTCITGYGSAMKVADFI
                     NSSLKNISKYNINLVPHNIESFKMYKNKFSNNKILAVVGSVDFNVANTPFIHIEELLN
                     GYGLDMLNKIIEGTFNANSITNIKETNLSYNSLIINFMTQNLELLDSVKLFNYVNSSL
                     LTIIELIDEELKSKFKLGYILHCSFMIERCFKNTTFPYNNITEIISKNHELYSLIREA
                     FKPTEAYFNIFIPDEEIAYIIDMINEYLNK"
     gene            3195..4439
                     /locus_tag="L3M60_003271"
     CDS             3195..4439
                     /locus_tag="L3M60_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBN6227902.1"
                     /translation="MEDEILKGKIKQLTILTLIFIFITPGFAFADTPPVPNSSRAALL
                     IDQETKRILFEKNIDEKMPLASLSKMMTFLLAIEAIDKNQVKETDMVKIDKSTASVGG
                     STCKLKDGDEISLGELMQGLMLVSGNDAALAIAKHIGKTEKNFVNMMNKKAEEIGMID
                     TYYFNPNGLPIYTDPEHKEPPIENISTAHDIVTLGKYMYDHYENQVTRITTMQVYNDT
                     KKDFTHYNTNPLLVSVPGVDGIKTGYTDNAGYCLAFSMMIPKDAKNERNHRLIGVVLG
                     DGNKKNRISSSASLLKYGKDNFHSKKIAHKGDIIETPCVDGIDDFKIKVKVDKDLYGV
                     VSDNENINPKVVFKNMNYPIHKGDIVGVAKYYNDSGKFVGSVNVKSESNIGCIPLKDK
                     IKIRVAKINRKLDVKNSVCFKV"
BASE COUNT         1808 a          608 c          695 g         1499 t
ORIGIN      
        1 tttatcattt ttttcatata tatcgactat acatcaatta tatcaaccat atattaataa
       61 atgatattac ataaattgaa atatattgaa aaacttaaat ttagttatgt aacaacataa
      121 tactctaatt taatctataa aaatctatac ctaatataat gcaactctta ttttaacacc
      181 atctattaca caaaacaaca tattaaaaag taaaatacaa aatatccact ataaacctta
      241 ttttaatact aaatatatat tataatgtat ttaatatagc ttaaatatat tttaaacata
      301 aagaaaggag acgatatatg ctaagaaaag ataaagttct attatcacta cataaattat
      361 gtgaaaatat gactttaaaa gaccttcagt cagataaaat aggattcact acaatagaaa
      421 ttgctaacca ttcatctctt gatagaagta ataccagtaa agaattaaat aacctgtata
      481 atgaaaaaaa agttctgaaa atacctggta agccaatttt atttctacat gctagtatta
      541 tagaaaattt aattggacgt actttgaaca gtaatgaatt ttctataaat aattgtaatg
      601 aactacttaa atcaagtaaa aatgacacag aagatatttt ttcaacatta cttggatatg
      661 attctagtct taaactacct atagaaaaag ctaaatctgc tatactctat cctccaaaag
      721 gtcttaatac attactcata gggcctactg gtgttggaaa aagtacattt gctgaaacaa
      781 tgtacaaata tgctgtagaa agtaaaatct tttcttctga aagcaaattc gtagtattta
      841 actgtgctga atatgcagaa aatgctaact tattattaag taacttattt ggttatgtca
      901 aaggttcttt tactggtgct aataaggata aattcggcat agttgcacag gctgatggag
      961 gaatattatt cttagatgaa atacacagat taccaccaga aggtcaagag atgctttttc
     1021 ttttgatgga caaaggcata tacagacgat taggagaatc tgacattgta cacaaagcta
     1081 atgtctttat tgtgtgtgcc acaacagaag atatacattc ttctctcctt gggacttttc
     1141 taagaagaat ccctgttaca attaacctac ccgctttaca ggataggtcc ttaaaagaaa
     1201 gattatctct tattaaatac ttctttatta tagagtcaaa atataccaac gcaactataa
     1261 gagttcacaa agatgtaatt aaatcattac tcctttacga ttgttttggt aatataggtc
     1321 agcttaaatc tgacatacag cttatgagtg caagagcctt tttaaattac aagactggac
     1381 ttaaggataa tatagaaata gatttatccc ttgttcctga ttacatctat attggtcttt
     1441 taaaattgaa tgaaaatcgt agtaatttaa ataaattatc agaaattgat tatatgcttt
     1501 tttatgaatt taatggtaac aacttagaag ttgaatatac aaaagaacta tctaaataca
     1561 atatagatac atcaaacaat ggatttaaat actctacaga gattccttat ctggaaaatg
     1621 atataaggag catgtttata aattacatgg aaaaatactc tacaaaatta ctttcgatat
     1681 cttcagtaac tcattctgat tatacaagta atgaaatttt taaagtaatc aatccaaaga
     1741 tttactatgc tgttgaacat tctttaaaag aggctgaaaa aatatcgaga aacaaatata
     1801 caaaacaaac ttatattgct ctttcaatgc atataagtgc acttatagaa aatataaatc
     1861 aagacacata taatcctaag tacaatttat ataatgataa aattttctta gataaagatt
     1921 taaatatagc taaaatagtt ttaagtataa ttgaaaacga cttacaaaca tctttaccta
     1981 aagatgaagt aaaatttata tccatgtttc taaatagcac gtatcttgaa ccaaacaatc
     2041 aatctgaaag tataggggtc ataatactcg ctcatggaaa ttcaacagca agcagtatgt
     2101 gtgatgtagc aaattcattg cttggtacaa accattgtaa tgcaattgac atgcctcttg
     2161 atgtaaaagt aagtactata cttgataaag ctatagaatt ggtaaaagaa tgtgacgaag
     2221 gtaagggtgt acttctttta acagatatgg gctctcttat agcttttgga gaacttattt
     2281 cagaaaaaac aaaaatcaaa actcgctcaa ttgaaatggt atctactcca atagttttag
     2341 aagcagtaag aaaaagtaca ctaccagaaa tgactttgga tgaattagct aattccttaa
     2401 gctctttaaa tccatatatt ggacgagcag caattgattc aacagaaata agctatcctg
     2461 aaaagtcaag agagtatgta ataataacga cttgtataac aggatatggt tcagctatga
     2521 aagtagcaga ctttataaat tcttcactta agaatattag taaatacaat attaatttag
     2581 ttcctcataa tatagaaagt tttaaaatgt ataagaacaa atttagtaat aataaaattc
     2641 tggcagttgt tggaagtgta gattttaatg tagccaacac cccttttata cacatagaag
     2701 aactactaaa tggatatggc ctagatatgc taaacaaaat aatagagggt acattcaatg
     2761 ccaatagtat taccaatata aaagaaacaa atctatcata taacagctta ataataaact
     2821 ttatgaccca aaacttagag cttttggact ctgttaaatt attcaactat gtcaattctt
     2881 ccttacttac aataatagaa ttaatagatg aagaattaaa gagcaaattt aagctaggat
     2941 atatactaca ttgctctttt atgatagaaa gatgttttaa aaatacaact tttccatata
     3001 ataacattac tgaaataatt agtaaaaatc atgaactata tagtttaata cgtgaagcct
     3061 ttaagccaac agaagcttat tttaatattt ttataccaga cgaagaaatt gcttatataa
     3121 tagacatgat taatgaatat ctaaataaat aaaacttata gcacttattt tgtgcatgtt
     3181 aattaatata tgaaatggag gatgagattt tgaaaggaaa aataaaacag ttaacaatac
     3241 ttacattaat ttttatattt ataactcctg gatttgcatt tgcagatact ccaccagtac
     3301 caaactcttc aagagcagca ctacttatag accaggaaac taaaagaata ttatttgaaa
     3361 aaaacattga tgagaaaatg ccattagcga gtttaagtaa aatgatgacc tttttattgg
     3421 caatagaagc aattgataag aatcaagtaa aagagactga tatggtgaaa atagacaagt
     3481 ctacagcttc agtaggaggt tctacttgta aacttaaaga tggtgatgaa atatctctag
     3541 gagaacttat gcagggtctc atgctagtat ctggaaatga tgctgctcta gccatagcta
     3601 agcatattgg taaaactgaa aagaattttg taaatatgat gaacaagaaa gcagaagaaa
     3661 taggaatgat tgatacgtat tattttaatc caaatgggct tcctatatat actgacccag
     3721 agcataaaga gcctcctatt gagaatatat caacagctca cgatatagtt acacttggaa
     3781 agtatatgta tgaccattat gaaaaccaag ttacaagaat aaccactatg caagtatata
     3841 atgatactaa aaaagatttt acacattata acacaaatcc tctacttgtt tcagtacctg
     3901 gtgttgatgg aataaagaca ggctacacag ataatgctgg atattgtctt gcattttcta
     3961 tgatgatacc taaggatgct aaaaacgaaa gaaatcatag attaataggt gttgtacttg
     4021 gtgatggtaa caagaaaaat agaatttctt cttctgctag tctattgaag tatggtaaag
     4081 ataatttcca ttctaaaaag atagcacata agggggatat tatagagact ccttgtgttg
     4141 atggtattga tgattttaag ataaaagtta aggttgataa agatttgtat ggtgttgttt
     4201 cagataacga gaatatcaat ccgaaggtag tatttaaaaa tatgaattat cctatccata
     4261 aaggagatat tgttggtgtt gcaaagtatt ataatgattc tggtaaattt gttggaagtg
     4321 tcaatgtaaa aagtgaaagt aatataggat gtattccttt gaaagataag attaagatta
     4381 gggttgctaa gataaataga aagcttgatg ttaaaaattc tgtttgtttt aaagtatagt
     4441 aaggcatact attaaaaaag tgtgttaaat ttataagact ccactttgtg gagttttttt
     4501 gttttttaca aaaaattaaa atagtggcaa caattgagga tgtgagggag agttgccact
     4561 attttgtata gggtattata ttatgttttt aagctagttt tactatcttc
//
LOCUS       DAFNNB010000197         4513 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0202, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000197 DAFNNB010000000
VERSION     DAFNNB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0202"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            159..458
                     /locus_tag="L3M60_003272"
     CDS             159..458
                     /locus_tag="L3M60_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227903.1"
                     /translation="MKWIKWYSFTCICIFIVVAFYMFIFPNKIETIDTSSAYSFVEKK
                     VPNSAVYQGYKNNPVDGTTTIYYSYDNSTHIVRLSHPEDSSREINWDKVSNISFD"
     gene            780..2435
                     /gene="ilvD"
                     /locus_tag="L3M60_003273"
     CDS             780..2435
                     /gene="ilvD"
                     /locus_tag="L3M60_003273"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBN6227904.1"
                     /translation="MRSDIKKGIEGAPKRALMYGMGLTKEEIERPLIGIVNAQNEVIP
                     GHLHLDEIAEAAKNGVRMSGGLPLEFPAIGVCDGIAMGHVGMNYSLASRELIADSIEA
                     MAMAHGFDALVLIPNCDKIVPGMLMAAARLNIPSIVVSGGPMLPGKKNGKVYDFNSTM
                     EGVGACKDGTVSEEELEELAMNSCPGCGSCSGLFTANSMNCLTEALGMGIPYNGTAAS
                     HSGERKRIAKYAGMYVMELLKNDIKPRDILTIDAFKNAIAVDMAMAGSTNTVLHLPAI
                     AYESGIELNLDFFDEISEKTPCLTKLSPSGKHHIEDLHMAGGIPAIMNELSKINGINL
                     DCKTVTGNTIGENIRDCKIENEDVIHTLKNPYSNQGGLAILKGNLALNGAVVKKSAVA
                     EEMLVHEGPARVFNSEEEAVNAIFSKKINKGDVIVIRYEGPKGGPGMKEMLSPTSAVA
                     GMGLDKHVALLTDGRFSGATRGASIGHISPEAMEGGLIGLVEEGDIISINIPDKKLEL
                     KVDEVEIEKRKLKFKPLEPKIKHGYLSRYAKLVTSANTGAVLK"
     gene            2776..3402
                     /locus_tag="L3M60_003274"
     CDS             2776..3402
                     /locus_tag="L3M60_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227905.1"
                     /translation="MNREEKSKNSKEKIIQSAFLLFSSKGYDSTSTQDIINLSGLSRG
                     AMYHHFKTKEDILRSVTKELYSQMNNFLENLVADNTLTANEKIIKLVVHSANDYTRRK
                     MLHCSWLEKIPFALIEEVRNLNNVVAPNIAKIIKQGVENKEFSCEYPQELAGMLVFSI
                     DILLDPVLFKREYSEVCNRLDFLLFMLKKMDIPLIDECGIQKFKDLFK"
     gene            3431..4243
                     /locus_tag="L3M60_003275"
     CDS             3431..4243
                     /locus_tag="L3M60_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBN6227906.1"
                     /translation="MFYNAKSCQMMIDSNTVNYIEFGSGKKILIILPGLGDGLSPLHG
                     KIQAIAFAFRYKRFAKDYKVYMFSRKNQITEKYSTRDMAKDQADIIKKLGIMKAKVMG
                     VSQGGMIAQYLAIDYPDLVEKLVLTVTSSKQNDTIQNVICSWIDMAKKQNYNDLMIDV
                     ANKSYSEKYLKKYRLFIPLLGKVGKPKDFNRFIIQATSCIEHNSFSELNKITCPTLII
                     GGANDKIVGNKASFHLAEKIKRSEIFIYEGLGHATYEEAQDFNERVLEFLNK"
BASE COUNT         1759 a          494 c          802 g         1458 t
ORIGIN      
        1 atttattaat ttataaaata tattatcaaa attaaaaagt atatataatc aagttttcta
       61 caactatata tacatgtaga aaaattttat cgtatgtata aatattataa atatatggaa
      121 ataataatag tatattttgg taaagtagga ggaattttat gaaatggatt aaatggtata
      181 gttttacatg tatatgtatt tttatagtag tagcttttta tatgtttata tttcctaata
      241 aaatagaaac tattgatact tcatctgcat atagttttgt tgaaaagaaa gtaccaaata
      301 gtgctgtgta tcaaggatac aaaaataatc cagttgatgg tactactaca atttattaca
      361 gctatgataa ttcaactcat atagttagat tatcacatcc agaagactct tcaagggaga
      421 ttaattggga taaagtttca aacatcagct ttgattaatc tttaatattt ataaaagtct
      481 atatgtataa ggtaaatata ttattaatct tattcatagt tgagcacttt ctattataga
      541 agtgcttttt ctgatttata gcataaaata ttactcaaca atttgtaaaa tatattagtt
      601 tataaaatat attgaaaaga taattatata atatgattgt gtaagatatt gataaaaaat
      661 cgtaataaat tttcattagt atatgctaat atttttttac aattttcgta attaattaat
      721 agaaaaatat attataataa tattatgaaa atttaaaaaa gtaagaaaga ggtaatatta
      781 tgagaagtga tataaaaaaa ggaatagaag gagctccaaa gagagcgtta atgtatggaa
      841 tgggtcttac taaagaagaa attgaaagac cattaatagg tatagtaaat gcacaaaatg
      901 aggtgattcc tggtcaccta catcttgatg aaatagcaga agccgcaaaa aatggagtaa
      961 gaatgagtgg tggtttgcca ttagaatttc ctgctatagg ggtatgtgat ggtatagcta
     1021 tggggcatgt cggaatgaat tattcccttg cttcaagaga attaatagca gattcaatag
     1081 aagctatggc tatggcacat ggttttgatg ctttggtact tatacctaat tgtgacaaga
     1141 tagtgccagg tatgttaatg gcagctgcaa gacttaatat accaagtatt gttgtgagtg
     1201 gaggtcctat gcttccaggt aaaaaaaatg gaaaagtata cgattttaac tctacaatgg
     1261 agggtgttgg agcatgtaaa gatggaacag taagtgaaga agaattagaa gagttagcta
     1321 tgaactcttg tccgggatgt ggttcatgtt ctggtctttt cacagcaaat agcatgaatt
     1381 gtttaacaga agctttaggc atgggtatac catataatgg tactgctgcg tcacattctg
     1441 gagaaagaaa aagaatagca aaatatgcag gtatgtatgt tatggagtta cttaagaatg
     1501 acataaaacc tagagatatt ttaacgatag atgcttttaa aaatgctata gctgtggata
     1561 tggcaatggc tggttctaca aatacagtac ttcacttacc tgcaatagct tatgaatcag
     1621 gaatagagct taacttagat ttttttgatg aaataagtga aaaaactcct tgtttaacaa
     1681 aattaagtcc aagtggaaaa catcatattg aagatttaca tatggcagga ggaataccag
     1741 ctataatgaa cgaactttca aaaataaatg gaataaattt agattgtaaa actgtaacgg
     1801 gcaatactat aggggaaaat ataagagatt gtaaaataga aaatgaagat gtaatacata
     1861 cattaaagaa tccatatagt aaccaaggtg ggcttgcaat attgaaagga aatcttgctc
     1921 taaatggagc tgttgtaaaa aagtcagcag ttgcagaaga aatgttagtt catgaaggac
     1981 cagcaagggt ttttaattca gaagaagaag ctgtaaatgc tatttttagt aaaaaaataa
     2041 acaaaggtga tgttatagtt ataagatatg aaggtccaaa gggcggtcca ggaatgaaag
     2101 aaatgttgtc tcctacatca gcagttgcag gaatgggact tgataaacat gtggcacttc
     2161 ttactgatgg tcgtttttca ggagcaacta gaggagcatc tataggtcat atttctccag
     2221 aagctatgga aggtggctta attggactag ttgaagaagg agatataatt tctataaaca
     2281 taccagacaa aaaattagaa ttaaaagtgg atgaagttga aatagaaaag agaaaattaa
     2341 aatttaaacc tttagaaccg aaaataaagc atggatactt aagtagatat gctaaattgg
     2401 taacatcagc aaatacagga gcagttttaa aatagtaaac tatatttatg caaattagaa
     2461 aaaattaaga aaatattaat tggatatctt aggtgttata gcactaatga gatatccttt
     2521 ttatttaaaa taaaaacaca gcaaaatctt gattatttct taaagtaatg catctctatt
     2581 aaaggaatat gagtataaca tatggatata tcttgtcaac attgttgagg taatacaact
     2641 ttttaaaggg ctgatgcaga ctaattttcg ttctccctaa actatgaaga aattaaaact
     2701 cagctaattc ttgacagaat aaatattaaa atacataatg aaaaacaaac agacagtctg
     2761 tttatggagg atgatatgaa tagagaagaa aaaagtaaaa atagtaaaga aaaaattatc
     2821 caatcggcat ttttgctgtt ttcatctaaa ggatatgatt caacatctac acaagatatt
     2881 atcaatttat ctggtctatc cagaggtgca atgtatcatc actttaaaac taaagaagat
     2941 atacttagaa gtgtcacaaa agaactttat tcacaaatga ataatttttt agaaaatctt
     3001 gttgctgata atacccttac agcaaatgaa aaaataataa aattggttgt tcatagtgcg
     3061 aatgattata cacgtagaaa aatgctacat tgtagctggt tagaaaaaat accatttgct
     3121 ttaatagagg aagttcgtaa tctcaataat gtagttgcac ccaatattgc taagataatt
     3181 aaacaaggtg ttgaaaataa agaattttct tgtgaatatc cacaagaatt agctggaatg
     3241 ctcgttttta gtattgatat tttacttgac cctgtattat ttaaacgtga atacagtgaa
     3301 gtatgtaata ggttggattt tttattgttt atgttaaaaa aaatggatat tcctctaatt
     3361 gatgagtgtg gtattcaaaa atttaaggat ttatttaaat aatgatagta ataaaagtga
     3421 ggaatagact atgttttata atgcaaaaag ttgtcaaatg atgatagaca gtaatacagt
     3481 gaactatatt gagtttggta gtggtaagaa gattctaatt attttaccag gattagggga
     3541 tggtttatct cctttacatg gaaaaataca ggcaattgct tttgctttta gatataagcg
     3601 atttgcaaaa gattataagg tttatatgtt cagtagaaaa aatcaaatta cagaaaaata
     3661 ttccacaagg gatatggcta aagatcaagc agatataatt aaaaaacttg gaattatgaa
     3721 agcgaaagta atgggtgttt ctcaaggcgg aatgatagct caatatctgg caattgatta
     3781 tcctgattta gttgagaaac ttgtgttaac agttacttct tctaaacaaa atgataccat
     3841 ccaaaacgtt atttgtagtt ggattgatat ggctaagaaa cagaattaca atgatttaat
     3901 gattgacgta gcaaataaat cttattctga aaaatattta aaaaaatata gattatttat
     3961 tcctctttta ggaaaagtag gtaaaccaaa ggatttcaat agatttatta ttcaagcgac
     4021 ttcttgcata gaacataatt ccttttcaga attaaataaa ataacgtgtc caaccctaat
     4081 cataggtggt gctaatgata aaattgttgg aaataaggct tcttttcatt tagcagaaaa
     4141 aattaaaaga agcgaaatat ttatctatga gggattggga cacgctacat atgaagaagc
     4201 acaggatttc aacgaaagag ttttagagtt tttaaataag taaatatatt tgttaaaaat
     4261 atctatgcca atgttgggaa tttaatatgg aaaatggaaa catgaaaagg cactggactg
     4321 gtactttcaa tatacctagc caatttctta tgtattatct actataaatg taaatataat
     4381 tttaatatgc ttatgtgttg gtgtattagt aaaacactta tacaaaaaga gaaattttgt
     4441 aaatatagat tataccaaca tttgttaact tatgaaaaga taatcaaaat ttttatgagc
     4501 tttttatata aaa
//
LOCUS       DAFNNB010000198         4503 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0203, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000198 DAFNNB010000000
VERSION     DAFNNB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0203"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..655
                     /gene="pgmB"
                     /locus_tag="L3M60_003276"
     CDS             <1..655
                     /gene="pgmB"
                     /locus_tag="L3M60_003276"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBN6227907.1"
                     /translation="DGVITDTAYYHYMAWKKLAHKIGINIDTKFNESLKGISRMESLD
                     RILEFGNKKDLFSKEEKLKMSEEKNNYYIALINDITSSDILPGIESLLIDVKSNNIKI
                     GLSSASKNAINVLNHLGISDKFDFIANAAKCKNNKPHPDIFLMSAKGLNVNPKNCIGI
                     EDASAGIDAINSANMFSVGVGNYENLKKANLVVDSTNQLKFEYIQEKYNEYMVRRII"
     gene            652..2937
                     /locus_tag="L3M60_003277"
     CDS             652..2937
                     /locus_tag="L3M60_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HBN6227908.1"
                     /translation="MKCLFSKIDEWKIIQDKIEIKENRLAESIMSIGNGYMGMRGNYE
                     ENYSGDTHKGSYIAGVWFPDKTRVGWWKNGYPEYFGKIPNSVNYIGIRIFIDDIELDL
                     AKCKVEDFHRELDMKNGILNRRFIVNINDKRFEARVTRFLSISVKELAVIKYSIKALN
                     SNAKIKFVPYLDSNIKNEDSNYEEKFWNGISSNSLEYKGSILSQTKENAFNTPVFTVG
                     SMMYIKTNGEVGAISHESNYNYCENSITMDALKNEVASIEKYVSITSTRDYKENELLR
                     KCEDILNRELSKNYDDILNEHTKLWNKRWESADVKINGDKSSQQGIRYNLFQLFSTYY
                     GEDSRLNIGPKGFTGEKYGGATYWDTEAFCVPVYLGVADKEVTRNLLEYRYNQLEQAK
                     ENAKKLGLKGALYPMVTFDGAECHNEWEITFEEIHRNGSIIYAIYNYTNYTGDYKYIK
                     EKGIDVVIEIARFWASRVHLSTKKDLYMIHGVTGPNEYENNVNNNWYTNYIAKWCLDY
                     AVENILRLEKEYNECIDRNNVTREEIDFWKIISKKMYLPYDEELQIIIQNDNFLDKEF
                     IEVKDLPKENLPLNQNWSWDKILRSCFIKQADVLQGIYYFGNRFTKEEKKRNFDFYEK
                     YTVHESSLSSSIYSIIASEIDNLEKAYELYSRTARLDLDNYNNDTNDGLHITSMSGSW
                     LSIVHGFAGMRTWDETLSFAPKLPKEWKSYSFNINYRDNIINVDVCSDLIKIENLKGK
                     AIKIIVYGRKYILEKYIKIDK"
     gene            3163..4056
                     /locus_tag="L3M60_003278"
     CDS             3163..4056
                     /locus_tag="L3M60_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBN6227909.1"
                     /translation="MKNKKFIVIILIISILIGVLVKEYSSREINKADINVSKYIKYAD
                     LASKNNVQVNWKYVASIVAVLNKNKLKNVKDSQIQEVSDLFVKNFSKNNKVNKLSYVL
                     DELKFNNRQKRLVDNYIDNLKDYGIKPERLKSDTKYMKFIDEIKTEAIQNYKDYKILP
                     SITIAQAIIESSWGKSTLAKQYNNLFGIKADAYWKGKSVTLETKEHLDTIIDDKFRIY
                     DDKNESIKDHAKFLATNKRYKSNGVFDAKTYIYQAKALEKAGYSTAKDENGNSIYAAR
                     LIELIQQYNLQLIDSEIQSEM"
BASE COUNT         1889 a          414 c          767 g         1433 t
ORIGIN      
        1 agatggtgta attacagaca ctgcatatta tcactatatg gcttggaaaa aactagcaca
       61 taagattggt ataaatatag atacaaaatt taatgaatct ttaaaaggca taagtaggat
      121 ggaatcatta gatagaatat tggaatttgg aaacaaaaaa gatttatttt caaaagaaga
      181 aaaattgaaa atgtcagaag aaaaaaataa ttattatata gcattaataa atgatataac
      241 ttcgagtgat attttgccag ggatagaaag cttgttaatt gatgtaaaat ctaataatat
      301 aaagatagga ctatcttctg caagtaagaa tgcaataaat gtattaaatc atttgggtat
      361 aagtgataaa tttgatttta tagctaatgc agcaaaatgt aaaaacaata aaccacatcc
      421 agatatattt cttatgtcag cgaaagggct taatgtaaat ccaaaaaatt gtataggtat
      481 agaggatgca agtgcaggta tagacgctat aaactcagct aatatgtttt ctgttggtgt
      541 tggaaattat gagaacttaa aaaaagcaaa tttagttgta gattcaacaa atcaattaaa
      601 gtttgaatat atacaagaaa agtataatga gtatatggtt aggagaatta tatgaaatgt
      661 ttatttagta agatagatga gtggaagatt atacaagata agattgaaat caaagaaaat
      721 agattagcag agtccataat gagtataggt aatggatata tgggtatgag aggaaactat
      781 gaggaaaatt atagtggaga tacacacaag gggtcttata tagctggagt atggtttcca
      841 gataaaactc gtgttggatg gtggaaaaat ggatatccag aatactttgg taaaattcct
      901 aattcagtca actatatagg tataagaatt ttcatagatg atatagaatt agatttagca
      961 aaatgcaaag tagaagattt tcatagagaa ttagatatga aaaatggtat tttaaatcgt
     1021 aggtttattg taaatataaa tgataaaaga tttgaagcta gagttactag gtttttaagt
     1081 atatctgtta aggaactcgc agtaattaaa tacagtataa aagcattaaa ttctaatgct
     1141 aaaataaaat ttgtaccata tttggattca aatataaaaa atgaagattc aaactatgag
     1201 gaaaaatttt ggaatggaat ttcttcaaat tctttagaat ataaaggaag tattctttca
     1261 caaacaaagg agaatgcatt taatacacca gtattcactg tgggctcaat gatgtatatt
     1321 aagacaaatg gtgaggtagg agctatatct catgagagta attataatta ctgtgaaaat
     1381 tcaataacaa tggatgcttt gaaaaatgag gttgcaagta tagaaaaata tgtatctata
     1441 acttcaacta gagactataa ggaaaatgag ttattaagaa aatgtgaaga tattttaaat
     1501 agagaactat ctaaaaacta tgatgatata ttaaatgaac atacaaagtt atggaataaa
     1561 agatgggaaa gtgcagatgt aaaaataaat ggagataaaa gtagccaaca aggtataagg
     1621 tataatttat tccaattatt ttcaacatat tatggagaag attcgcgatt aaatataggt
     1681 ccaaagggat ttactggcga aaagtatgga ggagctactt attgggatac agaagcattt
     1741 tgtgttccag tatatctagg tgtagcagat aaggaggtaa ctagaaatct tttagaatat
     1801 aggtacaatc agttagaaca ggcaaaggag aatgcaaaaa aactaggact taaaggtgct
     1861 ttatatccaa tggttacatt tgatggagca gaatgtcata atgaatggga gataacattt
     1921 gaagaaatac accgaaatgg aagtataata tatgcaatat ataactatac aaattataca
     1981 ggtgactata aatatataaa agaaaaggga atagatgtag taatagaaat agctagattt
     2041 tgggcaagta gagttcattt gtctacaaaa aaagatttgt atatgataca tggagttaca
     2101 ggcccgaatg agtatgaaaa caatgtcaat aataattggt atactaacta tatagctaaa
     2161 tggtgtttag attatgctgt agaaaatatc ttacgattag aaaaagagta taatgagtgt
     2221 attgacagaa acaatgtgac tagggaagaa attgattttt ggaaaataat atcaaagaaa
     2281 atgtatctgc catatgatga agaattacaa ataataattc aaaacgataa ttttttagat
     2341 aaagaattta tagaagtaaa ggatttacca aaagaaaatc ttccccttaa tcaaaattgg
     2401 tcttgggata agattcttag gtcgtgtttt ataaaacaag cagatgtatt acagggaatt
     2461 tactattttg gaaatagatt tactaaagaa gagaaaaaga gaaattttga cttctatgaa
     2521 aaatatactg ttcatgaatc ttctttatct tctagtatct attctattat tgcctctgaa
     2581 atagataact tagaaaaggc ttatgagtta tactcaagaa cagctaggct tgatttagat
     2641 aattacaata atgatacaaa tgatggactg cacataactt caatgtctgg ctcatggctt
     2701 tctattgtac atggatttgc aggtatgaga acgtgggatg aaacgctatc ttttgcccct
     2761 aagttgccaa aagagtggaa aagttattct ttcaatataa attatagaga taatattatt
     2821 aatgtagatg tatgtagtga tttaattaaa atagaaaatt taaaaggtaa agcaataaag
     2881 attattgtat atgggagaaa atatatatta gaaaaatata taaaaattga taaatagatt
     2941 taataatatt tttattatgt aaatagttaa aataaattta gctaattatg tatctatttt
     3001 aactattttg tatgatagaa aactatatat tataacaata gtagtgcttt taattaaata
     3061 tatttcataa atcttcataa aatcttcaaa atttcgtaaa gaatatgtca caaaagtaag
     3121 gtatattata ttcagcagat aatataaggg aggacaatat ttatgaagaa taaaaaattc
     3181 attgttataa tactgataat atcgatttta ataggtgtat tagtgaagga atattcatct
     3241 agagagataa ataaggctga tataaatgtc agtaagtata taaaatatgc tgatttagca
     3301 agtaaaaaca atgtacaagt caactggaag tatgtagctt ctattgttgc tgtgttaaat
     3361 aaaaataaac taaaaaacgt taaggattca caaatacaag aagtatcaga tttatttgtt
     3421 aagaattttt ctaaaaataa taaagtaaat aaattaagtt atgttttgga tgagttaaaa
     3481 tttaataata gacaaaaaag attggttgat aattatatag acaaccttaa agattatggt
     3541 ataaaaccag aaagattgaa atctgataca aaatatatga agtttattga cgaaattaag
     3601 acagaagcaa tacaaaatta taaggattat aaaatacttc catctataac cattgcacaa
     3661 gccataatag aatctagctg gggtaagtca actttggcaa aacaatataa taatctattt
     3721 ggtataaagg ctgacgctta ttggaaaggt aagtcagtga ctttagaaac gaaagaacat
     3781 ttagatacaa ttatagatga taaatttaga atatatgatg ataaaaatga atctataaaa
     3841 gaccatgcaa aatttttagc cacaaataaa agatataaat cgaatggtgt atttgatgca
     3901 aaaacatata tatatcaagc taaagcattg gaaaaggcag gttatagtac tgcaaaggat
     3961 gaaaatggaa atagcatata tgcagctcgt ctaatagaat taattcaaca gtataatctt
     4021 caattaatag acagtgaaat tcaaagtgaa atgtaacttt gattaaactt ttattgataa
     4081 ggctatgatt tttaaaatca tagccttatt ttgatatgtt ttcacttgta attattaaag
     4141 cgatattatt atctttcaaa atttctttta cagtacaaat caaattatct aagttaaaac
     4201 ttggagttat ttataaaagg attatttatt ttatataaat tttgcattta aataactctt
     4261 atttctttaa ggttgacagt tagtctataa aaattataat tccttataaa aaagtcaata
     4321 gatatataca ataaaaaatt ctcaaaaaga cttttacttg caaattgaaa ttaatgaagt
     4381 aaaaaagtat tctttattaa aagtagatgg caactatata gtgaataata aaaaatggta
     4441 atttgaaata atttttatga atagtattaa tatatagata cagggagatt gatgcgatta
     4501 tgt
//
LOCUS       DAFNNB010000199         4443 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0204, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000199 DAFNNB010000000
VERSION     DAFNNB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0204"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..2558)
                     /gene="carB"
                     /locus_tag="L3M60_003279"
     CDS             complement(<1..2558)
                     /gene="carB"
                     /locus_tag="L3M60_003279"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) large subunit"
                     /protein_id="HBN6227910.1"
                     /translation="MPKLDNIKKTLVLGSGPIIIGQAAEFDYSGTQACQALKEEGIEV
                     VLVNSNPATIMTDKEIADKIYIEPLTIEFIEKIIEKERPDSLLAGMGGQTGLNLAVEL
                     HDAGILDKYNVKVIGTSIESIKKGEDRDLFREVMKEINQPVIVSDIVTNLEAGLEFAN
                     KIGYPVVVRPAYTLGGTGGGIAENEEELREILSHGLQLSPVGQVLLEKSIKGWKEIEY
                     EVMRDGNGNCITVCNMENVDPVGVHTGDSIVVAPSQTLSDEEYQLLRKASIDIINAIE
                     VQGGCNVQIALNPHSLEYAIIEINPRVSRSSALASKATGYPIAKVAAKIALGYTLDEI
                     ENAVTKKTYACFEPTLDYVVVKIPKWPFDKFRHANRKLGTKMMATGEIMSIGSNFEAA
                     ILKGIRSLETGKYSLVHAPSEGRTLEELKARVVVPDDERLFDLAEMIRRGYKVEMISE
                     ITGVDKWFINKFKWIVEQEEKLKVLKIEDLDKEYLHQLKKKGFSDKGISDLMKISPEK
                     LYELRSLYNIQPVYKMVDTCGGEFEALSPYYYSTYEQYDEVVVSDKRKVVVLGSGPIR
                     IGQGIEFDYCSVHCVKSLRKMDIETIIVNNNPETVSTDFDTSDKLYFEPLTEEEVLNI
                     IEKEKPEGVILQFGGQTAIKLAKFLHEKNIPILGTDFSDIDAAEDREKFDDLLERLDI
                     NRPKGKGVWTTNEGVEIAKELGYPVLVRPSYVLGGQGMEITYNEEKLIQYLDDAFDRD
                     HKNPVLIDKYLTGREIEVDAICDKEDILIPGIMEHLERAGVHSGDSTTMYPSQNISDE
                     IKEKIVEYTKKIALDLNVLGMVNIQFIEFQNELYIIEVNPRASRTVPYISKVSGV"
     gene            complement(2751..3800)
                     /gene="carA"
                     /locus_tag="L3M60_003280"
     CDS             complement(2751..3800)
                     /gene="carA"
                     /locus_tag="L3M60_003280"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002845470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBN6227911.1"
                     /translation="MKARLILEDGTVFIGKAFGYLEESIGEVVFNTSMIGYGEVLTDP
                     SYYGQIVTMTYPLVGNYGINLSSAESEKVQVKGFIVREKSDSPSNFRCEIDIDQYLKQ
                     NKVIGLEGIDTRALTKILRNNGTMKGIITLEDSKLEDVKHKLDKFSNTEAVRTVTRKE
                     VEHIKGNGPKVAVMDFGVKRNILRSFIARGCDITVFPATTSPEDVLSINPDLIFLSNG
                     PGDPEDLEDVIENIKALIGKKPIVGICLGHQLLALALGGKTAKLKFGHRGGNHPVKDL
                     EEGKVFITSQNHGYYVSEVPEQMKVTHINLNDNTVEGMRHEKLDVYSVQYHPEACPGP
                     KDNDYIFDKFLELVK"
     gene            complement(4057..>4443)
                     /locus_tag="L3M60_003281"
     CDS             complement(4057..>4443)
                     /locus_tag="L3M60_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011862003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBN6227912.1"
                     /translation="VVLATVNNHDKDEFIEIAKDMKELGYTFVATEGTAKSLRENGIE
                     ADIVNRVEESRPNILDAIRNKQVDIVINTPTKGNDSTRDGFKIRRTAIEFSTEIMTSL
                     DTLKALVEVKKKHLNKDKLKVYNIAE"
BASE COUNT         1271 a          885 c          451 g         1836 t
ORIGIN      
        1 actccactta ctttgcttat atatggaact gttctactag ctctaggatt aacttcaatt
       61 atatataatt cattttgaaa ttctataaac tgtatattaa ccataccaag cacattaagg
      121 tctaaagcta tttttttagt atattctact atcttttctt ttatttcatc agatatattt
      181 tgacttggat acattgttgt actatctcca gagtgaactc cagctctttc taaatgttcc
      241 attataccag gtattaatat atcttcctta tcacatatag cgtctacttc tatttctcta
      301 ccagttagat atttatctat aagtactgga ttcttatggt ctctatcaaa tgcatcatcc
      361 aaatactgaa ttagtttttc ttcattataa gttatttcca taccttggcc accaagtaca
      421 taagatggtc taactagtac tggataacct aattcctttg caatttcaac accttcatta
      481 gttgtccata caccttttcc ttttggtcta tttatatcta gtctttctag taaatcatca
      541 aacttctctc tatcttctgc tgcatctata tcgctaaaat ctgttcccag tataggtata
      601 tttttttcat gtaagaactt agccagtttt atagctgtct gtccaccaaa ctgtaatatt
      661 acaccttctg gtttttcttt ttctattata tttaaaacct cttcttctgt taatggttca
      721 aaatataact tatctgatgt atcaaaatct gtacttactg tttcagggtt attgtttaca
      781 attatggttt ctatgtccat ttttcttaaa gattttacac aatgaactga acaataatca
      841 aattctatac cttgacctat tcttattgga ccagaaccca atactaccac ttttctctta
      901 tcactaacaa ctacctcatc atattgctca taagttgaat aatagtaagg tgataaagct
      961 tcaaattctc ctccacacgt atcaaccatt ttatatactg gttgtatatt atataaactt
     1021 ctcaattcat ataatttttc tggacttatc ttcattaagt cagatatacc tttatccgaa
     1081 aatccttttt tctttaattg atgtaaatat tctttatcta agtcttctat ttttaaaact
     1141 tttaattttt cttcttgctc tactatccat ttgaatttat ttataaacca cttatcaaca
     1201 ccagttattt ctgatatcat ttctacttta tagcctcttc ttatcatttc agctaagtca
     1261 aatagtctct catcatcagg cactactact ctagccttta attcctctaa tgttctacct
     1321 tctgatggtg catgaaccaa cgaatatttt cctgtttcaa gtgatcttat ccctttaaga
     1381 atagcagctt caaagttact acctatactc ataatttcac cagtcgccat catcttagtt
     1441 cctaattttc tattggcatg tctaaattta tcaaatggcc actttggtat ttttaccact
     1501 acataatcta atgttggctc aaaacaagca taagttttct tagttactgc attttctatc
     1561 tcatctaaag tgtagcctaa agctatctta gctgctactt ttgctattgg atatcctgta
     1621 gcttttgatg ctaaggctga tgacctactt actcttgggt ttatttctat tatcgcatat
     1681 tctaaactat gtggatttaa agctatttga acgttacatc caccttgaac ctctattgca
     1741 tttattatgt ctattgatgc ctttctaagt agttgatact cttcatcact tagggtttga
     1801 cttggagcaa caactatact atctcctgta tgaactccga ctgggtctac attttccata
     1861 ttacatacag ttatacagtt tccatttcca tctctcatta cttcatattc tatttctttc
     1921 caacctttta tactcttttc aagaagaact tgtccaacag ggcttaattg tagtccatgt
     1981 gaaagtattt ctcttaattc ttcctcattt tcagctatac ctccaccagt tccacctagt
     2041 gtgtatgctg gtcttacaac tactggatat cctattttat tggcaaactc aagacctgct
     2101 tctaaatttg taactatgtc actaactatt acaggttgat ttatttcttt cattacttct
     2161 ctaaataaat ctctatcttc tccctttttt atagactcta tagatgtccc tataactttt
     2221 acattatatt tatctaatat cccagcatca tgtaattcta cagctaaatt taatcctgtc
     2281 tgaccaccca tacctgcaag taaactatct ggtctttctt tttctattat tttttctata
     2341 aattctattg ttaatggttc tatatatatt ttatctgcaa tctctttgtc agtcattata
     2401 gtagctgggt tactatttac taatacaact tcaattccct cttcttttaa tgcttggcag
     2461 gcctgtgttc ctgaatagtc aaactctgca gcttgaccta ttattattgg tcctgaccct
     2521 aatactaaag ttttctttat attatctaat ttaggcataa ctctttctcc ttatctaaac
     2581 aatttttaca gctcttatct tcatcatcta tacaatcaat tttaattact ttatttcata
     2641 ttaagttcaa atttacgtat taatacatcc atttaataca tctatgttaa ttaaaccaaa
     2701 ctttttgatt taagttattc ttatctttta gttatctatt ctaatattat ttattttact
     2761 aactctaaaa atttatcaaa aatatagtca ttatcttttg gtcctgggca agcttcagga
     2821 tggtattgaa cactgtatac atctaacttc tcatgtctca ttccttcaac agtattatca
     2881 tttaaattta tatgtgtcac tttcatttgt tcaggaactt ctgaaacata ataaccatga
     2941 ttttgagaag ttataaatac ttttccctct tctaaatctt taactggatg atttccacct
     3001 ctatgtccaa atttaagttt agcagttttt cctccaagag ctaaagctaa aagttggtgt
     3061 cctaagcata ttccaactat aggtttttta ccaataagag cctttatatt ttctataaca
     3121 tcttctaggt cttctgggtc tccaggtcca tttgacaaga atattaaatc agggtttata
     3181 ctcaatacat cttctggaga tgttgtagct ggaaatactg ttatatcaca tccacgagct
     3241 ataaacgatc ttaatatatt tctttttacc ccaaaatcca taacagccac ttttggacca
     3301 tttcctttta tatgctctac ttcttttctt gtaacagttc taactgcttc tgtatttgaa
     3361 aacttatcca atttatgttt aacatcttct aatttactgt cttctaatgt aattatacct
     3421 ttcattgtcc cattatttct aagtatctta gtcaaagctc ttgtgtctat tccttcaagt
     3481 ccaataacct tattttgctt taaatattgg tctatatcaa tttcacatct aaaattactt
     3541 ggagaatcac ttttctccct tactatgaat ccctttactt gaactttttc agactctgct
     3601 gatgataaat ttataccata atttcctaca agaggataag tcatagtaac tatttgacca
     3661 tagtaagaag ggtctgttaa aacttcacca tatcctatca ttgaagtatt aaatactact
     3721 tctcctatac tttcttctaa atatccaaat gcctttccta taaaaactgt tccatcttct
     3781 aatattaacc ttgctttcat actacagtac ctcctataat tctctttaga taaactaaag
     3841 aacattaact aaatataagc ttttaaatat ctatatctat ttttcacttt tacattctag
     3901 ttttatactt atttgcattt attttttatc tttttctatt atactacaaa tttcttaaaa
     3961 aaaagaagtt atctcaaaag taaaaacccc tagaaaaaat aacctctttt tttaatttaa
     4021 taattaaatt cttaaactat acaatcaact taatttctat tcagctatat tgtaaacttt
     4081 taacttatct ttatttaaat gtttcttctt aacttctact aaagctttta gtgtatccag
     4141 agatgtcatt atttcagtag aaaactcaat agctgttctt cttatcttaa acccatctct
     4201 tgtggagtca tttcctttag ttggtgtatt tattactata tcaacttgtt tatttcttat
     4261 agcatctaat atgtttggtc tagactcttc tactctattt actatatcag cctctattcc
     4321 attttctctt aagctcttag ctgttccttc tgtagcaaca aaagtatatc ctaattcttt
     4381 catatctttt gctatttcta taaactcatc tttgtcatga ttgtttacag tagcaagtac
     4441 tac
//
LOCUS       DAFNNB010000200         4361 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0205, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000200 DAFNNB010000000
VERSION     DAFNNB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0205"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            87..2366
                     /locus_tag="L3M60_003282"
     CDS             87..2366
                     /locus_tag="L3M60_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6227913.1"
                     /translation="MLSDAKNNRYGLSIIAVISILVITISCLIYGSRLNKTLGEETNQ
                     YLSEIANQSVNVLKKQINGDIKLLESISIFIEGEEYFEVDNILSILKKQAINSSFKRM
                     GVILPNGTAYTTDGYREDFSKRDYFLKSMQGEVVITGKLKDVIEDDKNTNSDINVYSV
                     PIYKNNDVKGVIFATHSTKLYEKILSVPTFNGKGYSYVARSNGDIVLNPSSKNANSTK
                     ENLFSYIGKSDSISKSSINNMKSNIKNFKSGSLSYSMSGRGYYLSYAPIGINDWYLFS
                     EVPKAAISEKSYAIIKLTLSACVVLIIIFTALTIYILFIHKKSKEKLEEFAFKDNVTG
                     IGNSNKFNLEGGKFLSSHEKKNLVLIYFDIDKFKLVNDRFGYEEGDRVLKEIAEIIKN
                     IFKEQSVFSRISNDNFAIIFEKKNNRASIIEICELIRKKLSMIKTSLGVELNLIPSIG
                     VYFVEEGETNISTCLDKAMIAKTAVKRKYLKVYEIYAENLKEILIEERDIEQEMHDAL
                     KNEQFKVYLQPKVDLSTTKIVGAEALVRWQHPKKGLISPGIFIPIFEKNGFITELDMF
                     VFTQVCKNFKRWENENFPTFPISINLSRVHLENPDFISELERITKEYEIEPNLIEIEL
                     TESAIFDNTKILFKIMQTLKSVGFKISMDDFGSGYSSLNMLKDMPIDVLKLDRQFFIT
                     VGNAKKSQIVVSSIVQMAKQLDIKVVSEGVETVEQAEFLRLIGCDMAQGFLFARPMPI
                     EEYEKLILDRYSVKGKEIN"
     gene            complement(2976..4073)
                     /gene="ychF"
                     /locus_tag="L3M60_003283"
     CDS             complement(2976..4073)
                     /gene="ychF"
                     /locus_tag="L3M60_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBN6227914.1"
                     /translation="MKLGIVGLPNVGKSTLFNAITQAGAESANYPFCTIDPNVGVVSV
                     PDERLNKLQELYNSEKIVPTAIEFCDIAGLVRGASKGEGLGNKFLSHIREVDAIVHVV
                     RCFEDENVVHVDGSVDPLRDIETINLELIFSDIEILERRINKTQKAAKADKTLGSELD
                     LLKSIMSTLEESKCVRTMEFTEDEQIFVNSLDLLTSKPVIYASNVSEDDLADNGENNK
                     YVQQVKAFAETEDAEVVVVCAQIEAEISELDSAEEKKEFLETLGLEQSGLDKLIKSSY
                     ALLGLISFLTAGPKEVRAWTIKVGSKAPQAGGKIHSDIERGFIRAETIAFNDLVEHGT
                     MAAAKEKGLVKLEGKEYIVKDGDVILFRFNV"
BASE COUNT         1652 a          535 c          643 g         1531 t
ORIGIN      
        1 aaataagtat tattataaaa tgtttacaat aattgtcata agataatgat tatacataca
       61 tagatttgag aaaggcagga agcataatgt taagtgatgc aaaaaataat aggtatggtt
      121 tgtccataat tgctgtcata agtatcttag taattacaat ttcctgtttg atttatggtt
      181 caaggttgaa taagacatta ggtgaagaaa cgaatcaata tctttcagaa atagcaaatc
      241 aaagcgtcaa tgtgttaaaa aaacaaatta atggagatat aaagttacta gagtccattt
      301 caatatttat tgaaggagaa gagtattttg aagtagataa tatcctttct atactgaaaa
      361 aacaagctat taatagttca tttaaaagaa tgggcgttat tttaccaaat ggtacagcat
      421 acactacaga tggttataga gaagacttct ctaaaagaga ttatttctta aaatcaatgc
      481 aaggagaagt ggttataaca ggaaagttga aagatgtaat agaagatgat aaaaatacca
      541 atagcgatat taatgtatac tctgtaccaa tttataaaaa taatgatgtt aaaggagtta
      601 tatttgctac acatagtacg aagttgtatg aaaaaatact ttctgtacct acatttaatg
      661 gaaaaggata ttcttatgta gctagaagta atggtgatat tgttttaaat ccaagtagta
      721 aaaacgccaa ctctactaaa gaaaatttat tttcttacat tggtaaaagt gattcaatat
      781 caaaatctag tatcaataat atgaagtcaa atataaaaaa ctttaaatca ggttcattaa
      841 gttatagtat gtctggaaga ggatattatt taagttatgc tccaatagga atcaatgatt
      901 ggtatctttt ttctgaagtt cctaaagctg ctatatctga gaaatcatac gctattataa
      961 aacttacatt atcagcatgt gtagtgttga ttataatctt tacagcatta acaatttata
     1021 tactatttat acataaaaag agtaaggaaa aattagaaga atttgccttt aaagataacg
     1081 ttacaggcat tggtaattca aataaattta atcttgaagg gggaaaattt ttatcctctc
     1141 atgagaaaaa gaatctagtt ttaatttatt ttgatataga taagtttaaa ctagtaaatg
     1201 ataggtttgg gtacgaagaa ggagatagag tattaaagga aatagcagag ataattaaga
     1261 atatatttaa agagcaatct gtattttcaa gaatttcaaa tgataacttt gctataatat
     1321 ttgaaaagaa aaataataga gcatcaatta tagaaatatg tgaacttata cgtaaaaaat
     1381 tgtcaatgat aaaaacaagt ttgggagtag aactcaattt aattccatct ataggtgtgt
     1441 attttgttga agagggagaa accaatattt ctacctgtct tgacaaagct atgattgcaa
     1501 aaactgctgt aaaaagaaaa tatttaaaag tttatgagat ttatgctgaa aatttaaaag
     1561 aaattctcat agaagaacgt gatattgaac aagaaatgca tgatgcttta aaaaatgaac
     1621 aatttaaagt ttatttacaa cctaaagttg atttatcaac tactaaaatt gtaggcgctg
     1681 aagcgcttgt acgttggcaa catccaaaaa aaggtctaat aagcccaggt atatttattc
     1741 ctatttttga gaaaaatggt tttataacag aattggatat gtttgtattt acacaagttt
     1801 gtaaaaattt taaacgttgg gaaaatgaaa atttccccac atttcctata tcaataaact
     1861 tatcaagagt acatcttgag aatccagatt ttatttctga gcttgaaaga ataacaaaag
     1921 aatatgaaat tgagccaaat ttaattgaga ttgaattgac agaaagtgca atatttgaca
     1981 acacaaaaat attatttaaa ataatgcaaa ctttgaaatc tgttggattt aaaatttcta
     2041 tggatgattt tggttctggt tattcttctt taaatatgct taaagatatg ccaattgatg
     2101 tattgaaatt agataggcaa ttctttataa cagtaggaaa tgcaaaaaag agtcaaattg
     2161 tagtttcaag tattgttcaa atggcaaaac aacttgatat aaaagttgtt tcagaaggtg
     2221 ttgaaactgt ggagcaagca gagtttttaa ggcttatagg ttgtgatatg gctcaagggt
     2281 ttttgtttgc tagaccaatg ccaatagaag aatatgaaaa attaatatta gatagataca
     2341 gtgtaaaggg aaaagaaata aattagataa acttatgaaa taccatataa aaattaaaaa
     2401 aagtgaactt tgtttaatca atgttcactt tttttataaa aatgtactat tattaagaat
     2461 ataaattgtt aggaataaca agttacaagt tagatgatat ttgtttaaag tatatgaagt
     2521 attctgatat aaaactttat attaactgta gagtttatac aattaagtat atcaaaaatt
     2581 atttaaaaac ttgggaggga taacttgtat aaaattagaa aaatggcgga tgacaaagga
     2641 aggtttgtgt aagaacttgg taaattaagt tttggattgg tagggagttt ggcattaaaa
     2701 aacctaaaga tgcttttctt gatatagtgg ctggtcatat tgttggaatg gcttcttata
     2761 agatttttcc tgcaaagaat agaccaaaag tgggttattt agaaaccgtc tatgtaaaaa
     2821 agagagatat tctaaatgat ttataataat catttagaat atctctcttt taaaaaattt
     2881 tacataatta tacagagata ataaaaaatc tatcaataag tttaattttc taattatcat
     2941 acatataata gtcaaaatgt atatgtttaa tcatcttaaa cattaaatct aaataatatt
     3001 acatctccat ctttaactat gtattcttta ccttctaatt taactaaccc tttttcttta
     3061 gcagcagcca tagtaccatg ctcaactaaa tcattaaagg ctattgtttc tgctctgata
     3121 aatcctcttt ctatatcaga atgtattttt cctcctgcct gaggagcttt acttccaact
     3181 tttatagtcc atgctctaac ttcttttgga ccagcagtta agaatgaaat taaaccaagt
     3241 aaagcataag aagattttat aagtttatca agccctgatt gttcaagacc taatgtctct
     3301 aaaaactctt tcttttcttc agcagaatca agttcagaaa tttctgcttc tatttgtgca
     3361 catactacaa caacttcagc atcttctgtt tctgcaaatg ctttaacttg ttgaacatat
     3421 ttattatttt ctccattatc agctaaatca tcttctgata catttgaagc atatattaca
     3481 ggcttagatg taagtaaatc caatgaattt acgaatattt gttcatcttc tgtaaattcc
     3541 atagttctta cacacttact ttcttctaaa gtagacatta ttgattttaa taaatcaagt
     3601 tcagaaccta aagttttatc agctttggca gctttttgag ttttattaat tcttctttca
     3661 agaatctcta tatccgagaa aattaattct aagtttatag tttctatatc gcgaagtggg
     3721 tctacagaac catctacatg tacgacattt tcatcctcaa aacatctaac aacatgaact
     3781 attgcatcaa cttctcttat atgtgataag aatttatttc ctagaccttc tcctttagaa
     3841 gcacctctaa ctaaacctgc tatatcacaa aattctatag cagtaggaac tattttttca
     3901 gaattatata attcttgtag tttgttcaat ctttcatctg gaacagatac aactcctaca
     3961 tttgggtcta ttgtacaaaa agggtaattt gcagattctg cacctgcttg agttattgcg
     4021 ttaaatagtg tgcttttacc tacatttggt aaaccaacta tacctaattt catattaatc
     4081 gtcctctctc ttattctttt ttatattctt tatataactt taaacaatca atctatagga
     4141 ttgtaaaatt gttttaatta tagacataca tattgattat acatcatata tatcaagctt
     4201 gtaaactaga ctttaaatca tattgattta aataatattt gtatttaatt taaataaaaa
     4261 tattgctttt agaaaataat aaaaaggata taattaaata gaaggttttt atatgtgagt
     4321 atatgaaatt aaaaaataaa taaatttcaa ttttaaaatt g
//
LOCUS       DAFNNB010000201         4321 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0206, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000201 DAFNNB010000000
VERSION     DAFNNB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0206"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     repeat_region   86..2683
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=151..180
                     /rpt_unit_seq="gttaaacagtaacatgagatgtatttaaat"
     gene            2768..3262
                     /locus_tag="L3M60_003284"
     CDS             2768..3262
                     /locus_tag="L3M60_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HBN6227915.1"
                     /translation="MKITGTLINYYFHCKRQCWLLGNRINLEENSEDVKIGRLLHELK
                     EEKSKYKEIAIENIKIDKLTREYLVEVKKSDADIEAVKWQVLLYLKKLKEKGIVRKGK
                     IEFIEKNKTNKKIVFVDLSEVSEEQLISIEREIVNLINSDKAPCPDLKKGCKKCAYYE
                     YCYI"
     gene            3297..4295
                     /gene="cas1b"
                     /locus_tag="L3M60_003285"
     CDS             3297..4295
                     /gene="cas1b"
                     /locus_tag="L3M60_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-B CRISPR-associated endonuclease Cas1"
                     /protein_id="HBN6227916.1"
                     /translation="MGKTKYITSMGELTRKDNSICFRKNGRNVYIPVENTSEIFCLNE
                     VSLNSKLLDFLSRANIVVHFFNYYGGYSGTFYPKEYLISGKVTVAQVKTFEDRREEIA
                     KSIVNGIRINAINTLSHYYKHGNSEIQPLLSHLKKDSINKLEKSNNIKEILSVEGDIW
                     QTFYTSFKYILREEFIFNKRVKRPPDNPLNAMISFGNSILYSKTVTAIYNTHLNQSVS
                     FLHEPSESRFSLSLDLSENFKHVITFKTIFDLVNNRKINIEKHFDKNLNYCVLNDEGK
                     QIFINSLEERLESVKEHPKLKRKVSLKTCIKLDAYKMLKCIMEEKSFIPYNIKDGL"
BASE COUNT         1704 a          441 c          636 g         1540 t
ORIGIN      
        1 ttaattagaa ttagtttaaa gctattgaaa tatagatatt ttatatctta tatatatttt
       61 ctaggattag ctgggataaa aaatctgtat attagtgttt tcagtgtatt taagggaatt
      121 atatttttaa gaagtactga ttttaaagtg gttaaacagt aacatgagat gtatttaaat
      181 atgtttaagt cagatggctc accaaatcaa atgtttgtta aacagtaaca tgagatgtat
      241 ttaaatttta ttgtagctgt tataacttct ttatttgtat tagttaaaca gtaacatgag
      301 atgtatttaa attgatttat tagaggaaaa tagagaatta aatattaagt taaacagtaa
      361 catgagatgt atttaaatta ttgctctaaa tttagtagac caagttcaat ttctgttaaa
      421 cagtaacatg agatgtattt aaacttaaat gctttataaa gtattaattc attttgtaac
      481 gttaaacagt aacatgagat gtatttaaat tattaatcct tctattggaa aatatcgtct
      541 tcaagagtta aacagtaaca tgagatgtat ttaaattata gttttgtagc tctttcaagt
      601 tcatctgcat ttgttaaaca gtaacatgag atgtatttaa atatatatct aaatggatta
      661 ttatatctaa ctccatctgt taaacagtaa catgagatgt atttaaatat tttataatgg
      721 taaaaaagag ttttacatag ctggttaaac agtaacatga gatgtattta aatttttgaa
      781 gtttagttat atctgggtca ccagtctgtg ttaaacagta acatgagatg tatttaaatc
      841 agcatctaaa ccttctcttc ttgcttcctc tgaagttaaa cagtaacatg agatgtattt
      901 aaatttaaaa ttcaatttag taaatgaagt taattctgga gttaaacagt aacatgagat
      961 gtatttaaat ttttcttttg cagcacttcc tagaacatct ccatcagtta aacagtaaca
     1021 tgagatgtat ttaaattcaa atctaactga tgttctttta ttatcaaaag gttaaacagt
     1081 aacatgagat gtatttaaat tctttagctt tttctatttc agcaattatc tctactgtta
     1141 aacagtaaca tgagatgtat ttaaatggat tatccaatct ttaatcttat taccatacca
     1201 ttgttaaaca gtaacatgag atgtatttaa attatctctt aaagcactat ctaattcaac
     1261 aatagttggt taaacagtaa catgagatgt atttaaatta ttgaacttag catctaattt
     1321 agtattttta gtaggttaaa cagtaacatg agatgtattt aaattagaat aaaaattact
     1381 tgcagaataa aaaatgtctt cgttaaacag taacatgaga tgtatttaaa ttagaaaaaa
     1441 gtgtacattg acaactgaat atcccctgtt aaacagtaac atgagatgta tttaaataca
     1501 cttattttga ttttatttat tttaattttt tatgttaaac agtaacatga gatgtattta
     1561 aattccatct acttttttac ttttagcttt ttctttagtg ttaaacagta acatgagatg
     1621 tatttaaata gtctgaagat gagcgaatgt gctatatctg taatagttaa acagtaacat
     1681 gagatgtatt taaatttaaa tataataaat cgtaatgtac aaaaataact tagttaaaca
     1741 gtaacatgag atgtatttaa attttcttga gttaattgaa cccccatttt taagctccgt
     1801 taaacagtaa catgagatgt atttaaattt ttcttctagc tcttttttct tagccatttt
     1861 accagttaaa cagtaacatg agatgtattt aaatatgttt atacatgagt ttagtgatag
     1921 gttcttggtc gttaaacagt aacatgagat gtatttaaat atttcttttt gtgaagtgtt
     1981 aagagattgc tcttcgttaa acagtaacat gagatgtatt taaatacagt agctattcca
     2041 atttcttctg cttgtggcat agttaaacag taacatgaga tgtatttaaa ttcaaagatg
     2101 cccctctagc tggtttttgg gtatctagtt aaacagtaac atgagatgta tttaaattgt
     2161 gttgagtcgt gatattgact ttttcggaaa cgggttaaac agtaacatga gatgtattta
     2221 aatttgtagt ccttccattt ccatcacaca aaaagcatgt taaacagtaa catgagatgt
     2281 atttaaattc tatgttatgt acatgattac cttgtttgag taggttaaac agtaacatga
     2341 gatgtattta aattaagagt ctctttgtat atcaatttct ctagtagatg ttaaacagta
     2401 acatgagatg tatttaaatt aaattattag ttttaaaatt taaaggggga aaaaagttaa
     2461 acagtaacat gagatgtatt taaatgctaa cttaattact tgttccacat ttgctaatac
     2521 cgttaaacag taacatgaga tgtatttaaa taaatattca atatagtttt taaatacact
     2581 ttccattgtt aaacagtaac atgagatgta tttaaattca ttagtacttg ttattccagt
     2641 aaaagctact ttggttaaac agtaacataa gatgtatcta aattactcta tcataaaatt
     2701 ttaatatatt aaacaaacta atatgtgata tacttatgta taaataacaa aataaatagg
     2761 tgatgttatg aaaattacag gaactttgat aaattactat ttccattgta aaagacaatg
     2821 ctggttgctt ggtaatagaa taaatttgga agaaaatagt gaagatgtaa aaataggtag
     2881 acttcttcat gaactaaaag aagaaaaatc aaaatataaa gaaattgcaa ttgaaaatat
     2941 aaaaatagac aaattaacaa gagaatattt agtagaggtt aaaaagtctg atgctgatat
     3001 agaagctgtt aagtggcaag ttcttttata tttaaaaaag ttaaaagaaa agggaattgt
     3061 aagaaaaggg aaaattgaat ttatagaaaa aaataaaact aataaaaaaa tagtctttgt
     3121 agatttgagt gaagtatctg aagaacagct tatatctata gaaagagaaa ttgttaattt
     3181 aataaatagt gataaagcac catgtcctga tttaaaaaaa ggatgtaaaa aatgtgccta
     3241 ttatgaatat tgttatatat aaagaggtat aagaaacata taaggaggtg ttaatattgg
     3301 gaaagacaaa atatattacc tcaatgggtg aactcacaag aaaagataat tctatatgtt
     3361 ttagaaaaaa tggaaggaat gtatatatac cagtagaaaa tactagtgaa atattctgtc
     3421 taaatgaggt atctttaaat tcaaaattac tggatttttt atcaagagca aatattgttg
     3481 ttcatttttt taattactat ggtggatata gtggaacttt ttatcctaaa gaatatttaa
     3541 taagtggaaa agtaacagta gcacaggtaa aaacctttga agatagaaga gaagaaatag
     3601 caaaatccat agtcaatggt ataaggatta atgcaataaa tacattatcg cattactata
     3661 agcatggaaa ttcagaaata caaccattat tatcacatct aaaaaaagat tctataaaca
     3721 agttagaaaa atcaaataat ataaaagaaa tattatcagt tgaaggagat atttggcaaa
     3781 cattttatac ttcctttaag tatattttaa gagaggaatt tatttttaat aaaagagtta
     3841 agagaccacc agataatccc ttaaacgcaa tgatatcatt tggtaacagt attttatatt
     3901 ctaaaactgt aactgctata tataatacac atctaaatca aagtgtaagt tttttacatg
     3961 aaccaagtga aagtagattt tctttaagtc tagatttaag tgaaaatttt aaacatgtta
     4021 taacttttaa aactatattt gatttagtta ataatagaaa aataaatatt gaaaaacatt
     4081 ttgataaaaa tttgaactac tgtgttctaa atgatgaagg aaagcaaatt tttataaatt
     4141 ctttagaaga aagattagaa tcagtcaaag aacatccaaa attaaaacga aaagtaagtt
     4201 taaaaacctg tataaaatta gatgcttaca aaatgttaaa gtgtataatg gaagaaaaaa
     4261 gttttattcc atataacata aaagacggat tataatttaa acataaagta agtattttaa
     4321 t
//
LOCUS       DAFNNB010000202         4195 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0207, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000202 DAFNNB010000000
VERSION     DAFNNB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0207"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..831)
                     /locus_tag="L3M60_003286"
     CDS             complement(<1..831)
                     /locus_tag="L3M60_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HBN6227917.1"
                     /translation="MNFKKLSNVKVSVMAVVLSVGILSGYSALNKQVTLVVRGEERTI
                     STFSSNVGDLLESQNISYDANDIVTLDKNAKLSNGDKIEVIDVKEKTVVETKDIPFEV
                     TVVEDGSLLKGDSKVKEEGKSGKSELIYKETYHNGKKVEKEFVKKVVKAEPVNKVVSK
                     GTKVEMQVASSRGESSRRMASSSSSSSSSNNGKNMRVVATAYTGHSITATGTKPKWGT
                     IAVDPKVIPYGTKVYIPQFDMVFTAEDCGGAIKGNKIDIYMNDSKTVYNWGRKTIDIR
                     I"
     gene            complement(1079..1753)
                     /locus_tag="L3M60_003287"
     CDS             complement(1079..1753)
                     /locus_tag="L3M60_003287"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBN6227918.1"
                     /translation="MVNLGNDWDELLKEEFEKDYYLNLRKFLIEEYKTRQIFPNMHNI
                     YEALKHTSYKDTKVLILGQDPYHGDNQAHGLAFSVQPQVKTPPSLLNMYKELKDDLGC
                     FIPNNGYLMPWADQGVLLLNTALTVRAHEANSHKNKGWEIFTDRVISILSEREDPVIF
                     VLWGSNARKKVELIDTSKHYILEAPHPSPLSASKGFFGCKHFSKINEILKKLGKEPIN
                     WQIENI"
     gene            complement(1790..2161)
                     /locus_tag="L3M60_003288"
     CDS             complement(1790..2161)
                     /locus_tag="L3M60_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HBN6227919.1"
                     /translation="MKKKDILLILGILVVITACYGIINVINSKNAGNIEIYVDNKLYK
                     TVSINAKEEFKIENRGGYNIVKIHDKGVEIVDASCPDKVCVHTGFINKPSQSIVCIPN
                     RVSIKINTNEKNDNQEDIISK"
     gene            2228..3283
                     /locus_tag="L3M60_003289"
     CDS             2228..3283
                     /locus_tag="L3M60_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HBN6227920.1"
                     /translation="MNKKKITFIFVILILALILFSFFRSSGQNTKYSKTSYYLGTVNE
                     VTVFNVKESKSDKILNECDSILRYIDNKMSTHIPGSDVSKINDNAGKKFVKVSDDTFF
                     VVKEAIQYSKLSDGYFDITIGPLSNLWAIGTDKAKVPSDSEIQKLLPLIDYKNIILDE
                     KNKSIKLNKENMKIDLGAIAKGYAADKIVSYLKSEDIKKGLVNLGGNIFTLEDSKNDK
                     PFKIGIQDPTSKNGESIGNIETTNKSVVTSGIYERFIEKDGKIYHHMLNPFTGYPFEN
                     NLSSVTIISDKSINCDALSTSTFGLGLEKGMDLINRIDNVDAIFITKDKKVYLTKGIK
                     DNFKLTDKAFSIEKLNK"
     gene            complement(3459..3893)
                     /locus_tag="L3M60_003290"
     CDS             complement(3459..3893)
                     /locus_tag="L3M60_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerC"
                     /protein_id="HBN6227921.1"
                     /translation="MEKRNLRIGIVYVVLGVTFLFSCLMTKDKLSSLLVGLAAGFGFN
                     GISIIYRYFYWNKHKEEYREKLEEEKINLQDERNVMYRDKAGRYAYIVCMIIIPISAF
                     VFAVLNALDIYNSLVIIIYLSVLWIVLYVVGVIYYRKLKNNE"
     gene            complement(3897..4094)
                     /locus_tag="L3M60_003291"
     CDS             complement(3897..4094)
                     /locus_tag="L3M60_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005809912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227922.1"
                     /translation="MKNCVEALRKKKGLNQEDFAKILKVSRQTISSIENGKYNPSLEL
                     AFQISDFFEMSIEEIFLWKEI"
BASE COUNT         1440 a          679 c          480 g         1596 t
ORIGIN      
        1 tatacgtata tctatagttt ttcttcccca gttataaact gtttttgaat cattcatgta
       61 gatatcaatt ttatttcctt ttatcgctcc accacagtct tctgcagtaa acaccatatc
      121 aaattgaggt atataaactt tagttccata tggtataacc ttagggtcta cagcgattgt
      181 tccccactta ggttttgttc ctgttgcagt tatactgtga ccagtatatg cagttgctac
      241 cactctcata tttttaccat tgttactact actgctacta gatgatgatg aagccattct
      301 tcttgagctt tctccccttg atgaggctac ttgcatttct acctttgtcc ctttacttac
      361 aaccttattt actggctcag ctttaacaac ctttttcaca aattcttttt ctactttctt
      421 tccattgtga taagtttctt tatatattaa ttcactttta ccagactttc cttcttcttt
      481 aaccttagaa tcacccttta ataaagagcc atcttcaaca actgtaactt caaatggaat
      541 gtctttagtt tctacaactg ttttttcctt tacatcaatt acttctattt tatctccatt
      601 agataacttt gcatttttat ctaaagttac tatatcattt gcatcatagc taatgttctg
      661 agattctaaa aggtctccta cattagaact gaatgttgat atagttcttt cttctcccct
      721 tactactaat gttacttgtt tattaagcgc agagtatccc gataatattc caactgataa
      781 aacaactgcc attaccgaga ctttaacatt actcaatttt ttaaaattca tataaaattt
      841 cctccctaag attttttaca ctatatattt tgcttcataa aattctgctt tgtaagtttc
      901 tttttttatt tgtgtttttt gtgtaactaa attgtaacta ttttttcaag agttgtcaac
      961 ttgacaaaaa aatataaata tgcatatata gttttttcaa ctatttaagc atatttctat
     1021 tagttacaac aatgtcattg tttattgtgt tttgttatca ttttgttacc ttttactttt
     1081 atatgttttc tatctgccaa ttaataggtt cttttcctag ctttttcaaa atttcattta
     1141 tcttagagaa gtgtttacat ccaaaaaaac ctttacttgc tgacaatggg cttggatgag
     1201 gtgcttctaa aatataatgt ttacttgtat caatcaattc cactttttta cgtgcattac
     1261 ttccccataa tacaaaaata actgggtctt cacgctcact taaaatagat attactctat
     1321 cagtgaaaat ttcccatcct ttgttcttat gagaattagc ttcatgcgct ctaacagtaa
     1381 gagcagtatt taaaagtaac actccttggt ctgcccaagg cattaaatat ccattgttag
     1441 gtataaaaca ccctaaatca tcttttaatt ctttgtacat atttaataga gatggtgggg
     1501 ttttcacttg tggttgaact gaaaaagcta aaccatgagc ttgattatct ccatgataag
     1561 ggtcttgtcc taatattaaa acttttgtgt ctttgtatga agtgtgcttt aaagcttcat
     1621 atatattatg catatttgga aaaatttgac gtgttttata ctcttctata agaaattttc
     1681 ttaaatttaa gtagtaatct ttttcaaatt cttctttcaa taattcatcc caatcgtttc
     1741 ctaagttaac cataactatc accaattcct ttcaattatt tttattatgt tatttagaaa
     1801 ttatatcttc ttgattatca ttcttttcat ttgtatttat ttttatgcta actctattag
     1861 gtatacaaac tatactttga cttggcttgt ttataaatcc agtatgtaca caaaccttat
     1921 caggacatga agcatctact atttcaaccc ctttatcatg aatctttact atgttatacc
     1981 cccctctatt ctctatttta aattcttctt ttgcatttat tgaaacagtt ttatacaatt
     2041 tattatctac ataaatctca atatttccag catttttaga atttataaca tttattatcc
     2101 cataacatgc tgtaataaca accaaaatgc ctaaaataag aagaatgtct tttttcttca
     2161 ttttttgtcc tcctatgaaa ttatgttata ataaaataat aacacaattt cataggagga
     2221 atataccatg aataagaaaa aaatcacatt tatttttgtg atattgattt tagcattaat
     2281 tttattttca ttttttagga gttcaggcca gaatactaaa tatagtaaga ctagctacta
     2341 ccttggtaca gtaaacgaag ttactgtatt taatgtaaaa gaatccaaaa gtgataaaat
     2401 cttaaatgaa tgtgattcaa ttttaagata tattgacaac aaaatgagca ctcatatacc
     2461 aggaagtgat gtttctaaaa taaatgataa tgctggtaag aaatttgtca aagtttccga
     2521 tgatacattt ttcgtcgtaa aagaagctat acaatattcc aaattatcag atggatattt
     2581 tgatataaca attggtccat tatcaaattt atgggcgatt ggaacggata aagctaaagt
     2641 tccaagtgat tctgagatac aaaaacttct gcctcttatt gattataaga acatcatact
     2701 ggatgaaaaa aataaatcta taaaattaaa caaagaaaat atgaaaattg atttaggtgc
     2761 catagctaag ggatatgctg ctgataaaat tgtatcctat ttaaaatcag aggatataaa
     2821 aaaaggttta gtaaacttag gaggaaatat attcacttta gaagacagta aaaatgataa
     2881 accttttaaa ataggtattc aagacccaac tagtaaaaat ggtgaatcta taggaaacat
     2941 agaaacaaca aataaatctg ttgttacttc tggaatttat gaaagattta tcgaaaaaga
     3001 cggaaaaata tatcatcata tgttaaatcc atttacaggg tatccttttg aaaacaattt
     3061 aagtagtgtt acaataatct ctgataaatc tattaattgt gatgccttat ccacttctac
     3121 atttggactt ggacttgaaa aaggtatgga tttaattaat agaattgata atgtagatgc
     3181 tatattcata actaaggata agaaagtata cttaactaag ggtataaaag ataattttaa
     3241 acttacagat aaagcctttt ctattgaaaa attaaataaa taataagttt aaataataac
     3301 tttacctatt ataaatacaa aatacatatg aatttaaata gttattaaat aactaatgca
     3361 aatttaaaat ttatataaaa tgtggtatat acttaagtag tctaaaacaa attgtactta
     3421 tgtatatacc acattaatag cttaaattta tataaatact attcattatt ttttaacttt
     3481 cgatagtaaa taactcctac aacatacaaa acaatccata aaacagatag atatataatg
     3541 attactaatg agttgtatat atctaaggcg tttagtacag cgaaaacaaa agcagatata
     3601 ggaattataa tcatacacac aatataagca tatcgtcctg ctttatctct atacattaca
     3661 ttcctttcat cctgaaggtt tattttttct tcttctagct tttctctata ttcttcctta
     3721 tgcttattcc agtaaaaata tcgataaatt atagaaattc cattaaatcc aaatcctgct
     3781 gctaaaccaa caagcaaaga acttaactta tccttagtca ttaaacaaga aaacaagaaa
     3841 gtaactccta aaactacata tactattcct attcttaaat tccttttttc cataaattat
     3901 atctccttcc ataaaaagat ttcttcaatc gacatttcaa aaaaatcaga tatctgaaat
     3961 gcaagttcta aagatggatt atattttcca ttttcaatag aactaatcgt ttgccttgaa
     4021 accttaagaa tttttgcaaa gtcctcttga ttaagacctt ttttctttct caaagcttct
     4081 acacaatttt tcaaaaatat ctcctccttc attgtaaagt ttcctttaca catataatat
     4141 atcatattac tattttattg taaaggaaac tttacaataa tcatataaaa ataga
//
LOCUS       DAFNNB010000203         4088 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0208, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000203 DAFNNB010000000
VERSION     DAFNNB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0208"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(40..345)
                     /locus_tag="L3M60_003292"
     CDS             complement(40..345)
                     /locus_tag="L3M60_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227923.1"
                     /translation="MPSMIMEIVGVLFIAIGLILTFIEIKGNFPSFYGFGQNSIGKNA
                     IGIIAAGIALIFFRNGIDSIGSIATYGIFLAIELVVIVAYFLIRKKFTKEDKHLRTN"
     gene            complement(1070..1288)
                     /locus_tag="L3M60_003293"
     CDS             complement(1070..1288)
                     /locus_tag="L3M60_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227924.1"
                     /translation="MSVLLKTKIKSYREKNLMTQGELAKSVGVRRETIVHLENGRYNP
                     SLKLAMDIAKVFDTTVEELFEFIEENDI"
     gene            complement(1289..1696)
                     /locus_tag="L3M60_003294"
     CDS             complement(1289..1696)
                     /locus_tag="L3M60_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227925.1"
                     /translation="MSKQQTGIKSTLISEILGISYCIGATLVNKYFFKGSSIEYLPII
                     IWVLAMGAQWVYNKKYDDLIDEASKCILSRVNDIAIKLLFFSVAIVSIFLVTLDINVS
                     NLNIGMSLLVILFIQSLLKLILFIYFDRKGIYN"
     gene            complement(1782..2270)
                     /locus_tag="L3M60_003295"
     CDS             complement(1782..2270)
                     /locus_tag="L3M60_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3841 domain-containing protein"
                     /protein_id="HBN6227926.1"
                     /translation="MKFYTIQTLEFWNNNKNNKYLSNDYSFILEDFDIPYKWMYSQMT
                     NRIYEFDDSMIWVWTKRPDLRCSGYLEKGKKGVLLEIEISEDNVLLSEFDLWHLPLMD
                     ITVELYEDEQIEKKVSWERIFDLELCSKLNEVNIEDLTLQGVTSKVPLYNVKLIKEFV
                     AK"
     gene            complement(2450..3547)
                     /locus_tag="L3M60_003296"
     CDS             complement(2450..3547)
                     /locus_tag="L3M60_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBN6227927.1"
                     /translation="MKEKLEEKSKIIKTSKEYKRTVIEEKDREWTKKLNDILSRDDFS
                     NEKLDKIKNLIPKEILTSENVGEVVTEDGYAKPEYDEVFKSLFTLNNDYDLLANFIND
                     ILKDAPYANRNIKPFTQIKRIIKVETDPTINYIGEKRPRLDILAEDEESNHINIEMQR
                     ALEEDYLERAEYYLSRVHGRKLEEGKEYKEIGKTVGIHILSHVKYNHIEDYVNCLRLT
                     IDGHPDIFSSKTALYFIELPKIRKSSCIANRVLIWGKLIDNPSHIDIRILSKTDYVIK
                     RVLDRLKELGSNEDYLLNLKRGAYIMNKSRNFKEEIFQEGVEKGIAKGKREERFNIII
                     KLKNKGYNLSEICDIIDDLNKSEVEKIYNQN"
     gene            complement(3920..>4088)
                     /locus_tag="L3M60_003297"
     CDS             complement(3920..>4088)
                     /locus_tag="L3M60_003297"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227928.1"
                     /translation="DSNNDTIKSTVKKGISFGSCLAMIISYTAWKSIPWAIFHGLMSW
                     IYVLYYWVKYA"
BASE COUNT         1375 a          646 c          368 g         1699 t
ORIGIN      
        1 tttgttttta attttccaat tttatagtag ataatttagt taattagttc ttaaatgttt
       61 atcttcttta gtaaattttt ttcttattaa gaaatatgca actattacaa caagttctat
      121 agctaaaaat ataccataag tagctataga acctatgcta tctatgccat ttctaaagaa
      181 aattaaagca attcctgctg ctataatacc tatagcattt ttccctatag aattttgacc
      241 aaaaccataa aaagaaggga aatttccttt tatttcaatg aatgtcaaga ttaatccaat
      301 agctataaat agtactccta caatttccat aatcatacta ggcattttga acactcctta
      361 ttaataatta actactacta taaaaatatt tattattttt ataattatat tatatatgcg
      421 attagatgtt tttggaagta tatttggaag taatttcata tttatttaac ttaatttttt
      481 tgtgataaaa tatttctaat tttcatatag atattattat aaattttcta gtttatggag
      541 ctattcattc tataattcac aaatatatat tttttaatct attaaactat tcattttctg
      601 cttataagca tggacttatt atctataagc ttggtttttc atctataagt catttattct
      661 ttaatctatg agcttgattt ttcatctata agtcatttat tttttaatct atgagcttgg
      721 tttttcatct ataagtcatt cattttttaa tctatgagct tggtttttca tctataagtc
      781 atttattttt taatctatga gcttgatttt tcatctataa atcattcatt ttttaatcta
      841 taagcttggt ttttatttat aagttattta ttctctaatc tataaggttg cttcttcatc
      901 tataaatcat tcattcttta atccattagc ttggtttttc atctattaat catttattct
      961 ctaatttata agcttggttt tttacctata attaattcat tctctaatac ataaacttga
     1021 attttttatc tattaattca ttctctaatg cataagcaat acatgaaatt taaatatcat
     1081 tttcttcaat aaattcaaat aactcttcta cagttgtatc aaatacctta gcaatatcca
     1141 ttgccagttt taaagaagga ttgtagcgac cattttctaa atgtacaata gtttcacgtc
     1201 ttactcctac tgactttgca agttcacctt gtgtcataag gttcttttct ctataggatt
     1261 taatttttgt ctttagtaat actgacacct aattgtatat cccctttctg tcaaaatata
     1321 taaataaaat taacttcaac aaggattgaa taaacaaaat gactaataaa gacattccta
     1381 tatttaaatt tgacacattt atgtccaaag ttacaagaaa tatagatact atagctacag
     1441 aaaaaaacaa taattttatt gcaatatcat taactcttga tagtatacat tttgatgcct
     1501 catcaattaa atcatcatac tttttattat agacccactg agctcccata gctaataccc
     1561 aaataattat aggaagatat tcaatactac ttcctttaaa aaaatactta tttactaatg
     1621 tagctccaat acaataagaa attcccaata tttctgaaat aagtgttgat ttaataccag
     1681 tttgttgttt agacatataa aatccccctt aaaaagttat atatttataa cacctcttgt
     1741 tataaatata taactttttt gaataaattt caataaaaat attattttgc aacaaattct
     1801 tttattaatt ttacattata tagaggaact ttagatgtca ctccttgcaa tgtcaaatct
     1861 tctatattaa cttcatttaa ttttgaacac agttctaagt caaatattct ttcccaactt
     1921 accttctttt caatttgttc atcttcataa agctctacag taatatccat taaaggtaaa
     1981 tgccataaat caaattctga taataacaca ttatcttcac ttatttctat ttcaagtaaa
     2041 acaccttttt ttcctttttc taaataacca ctacatctta aatcaggtct cttcgtccaa
     2101 acccatatca tactatcatc aaattcatat attctatttg tcatttgact atacatccat
     2161 ttgtatggaa tatcaaagtc ttctaatata aaagaataat catttgatag atatttattg
     2221 tttttattgt tgttccaaaa ttctaaagtc tgtatagtat aaaatttcat atataaatca
     2281 atccctttgt ttttctataa gaaggagatg gttcaaactt ttattttaaa tcatctcctt
     2341 agcttatata tctctttgta tctactatag ttaaataaca aggttttata ttaatttaaa
     2401 tttaaaagag gaagctttgt atttatctag tgccatctca attatagtat tagttttgat
     2461 tatagatttt ttcaacctct gatttattca aatcatctat aatatcgcat atttcactca
     2521 aattgtatcc tttatttttt aattttatga tgatattaaa tctctcttct cttttccctt
     2581 ttgcaatacc tttttcaaca ccttcttgaa aaatttcttc tttaaaattt ctacttttat
     2641 tcataatata cgcacctctc tttaaattta ataaatagtc ctcattactg cctaattctt
     2701 ttaatctatc aagtactctt ttaataacat aatccgtttt agatagtatt ctaatatcga
     2761 tatgagaagg attatcaata agtttacccc atattagaac tctatttgct atacaactgg
     2821 atttacgaat ttttggaagt tctataaaat ataatgcagt tttactagag aaaatatctg
     2881 gatgcccatc tatcgttaat ctcaggcaat ttacataatc ttctatatga ttatatttta
     2941 catgacttag tatatgtatt ccaacagttt ttccaatttc tttatattct ttaccttctt
     3001 ctaactttct accatgtaca cgactaagat aatattcagc tctttccaaa taatcctctt
     3061 caagagctct ttgcatctca atatttatat gattactttc ttcatcttca gccaaaatat
     3121 caagtctagg tcttttctca ccaatgtaat taattgttgg gtcggtttca accttaatta
     3181 ttctttttat ttgagtgaac ggtttgatat tcctattagc atatggagca tctttgagaa
     3241 tatcatttat aaaatttgca agtaaatcgt aatcattatt taaagtaaat aatgatttaa
     3301 atacctcatc atactcaggc ttagcatacc catcttcagt tacaacttct ccaacatttt
     3361 cactagtcaa tatttctttt ggaattaaat tcttgatttt atctaatttt tcattagaaa
     3421 aatcatctct actaagtatg tcatttaatt ttttagtcca ttctctgtcc ttttcttcaa
     3481 ttacagttct cttatactct tttgatgttt taataatctt agatttttct tctaattttt
     3541 ctttcatatc aacacctctt ttttattatt acccatttct tcataccaaa gtctgcactt
     3601 ctaagtaaaa tttgcattat tgaaataaga acatgataaa ctcatatatc attactaaag
     3661 ttaagacttt aaagagaatc acttagatta caatagattt tagataactt tctttttaaa
     3721 actattgttg ataaatgaat gaatataaag gtatgtatta aaatagagat aataatcttt
     3781 ttcattgttt aagtaatgaa aatatagttt atttaataca aaaaagagtg ccttataaac
     3841 taaaagttca cttcgagaca ctcccttaca ttttgtttct taaacttcac aatattacac
     3901 acttatttat taatttaatc tatgcatact taacccaata ataaagtaca tatatccaac
     3961 tcattaagcc atgaaaaata gcccatggaa tagatttcca tgcagtataa gaaataatca
     4021 ttgctaaaca agaaccaaaa gaaataccct ttttaacagt tgattttata gtatcattat
     4081 ttgaatcc
//
LOCUS       DAFNNB010000204         4033 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0209, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000204 DAFNNB010000000
VERSION     DAFNNB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 4033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4033
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0209"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            315..1223
                     /gene="trxB"
                     /locus_tag="L3M60_003298"
     CDS             315..1223
                     /gene="trxB"
                     /locus_tag="L3M60_003298"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBN6227929.1"
                     /translation="MVDIIVIGAGPAGLTSAIYAMRAGLSVTVFEKNIYGGQVASTSE
                     VENYPAVQKISGVEFSNNIYNQAVAQGVDIQFDEVEEINLEGKIKIVKTSSAEHKAKA
                     VILANGVERRKLGCVGEQEFTGRGVSYCATCDGAFFKDKEVAIVGGGNTALEDALFLA
                     NNCTKVYLIHRRDSFRGEEVLEKSVKARKNIEILYSHGVEKIEGEKTVSKIEVKNLKT
                     EEKRTIDVSGIFIAIGLKPNNKMFENVLDLDEGGYIISDESCTTSVEGVYVAGDSRTK
                     FLRQIITAASDGAIAAVQAANYINVE"
     gene            complement(1313..3136)
                     /gene="typA"
                     /locus_tag="L3M60_003299"
     CDS             complement(1313..3136)
                     /gene="typA"
                     /locus_tag="L3M60_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HBN6227930.1"
                     /translation="MSQKHKIINIAVIAHVDAGKSTLVDAFLSQSGVFRKNEVVKDCV
                     MDSNDLEKERGITIYSKNCAINYEDYKINIVDTPGHSDFSSEVERVMKTVDTVILLVD
                     ASEGPMPQTRFVLQKSLEFGLKPILFINKIDKKDQRAEEVVNEVFDLFVDLNATDEQC
                     EFPIIYGIAKQGIAKLEMDDDSEELSPLFKTIVNHVEAYPDYDNEPLQFQISALAYDD
                     YVGRLGIGRIYKGTLKNNTQVAICREDSVVSKGKVSKLSVYEGLKQVEVDEATSGEIV
                     VIAGIPDISIGETICDLDSPLPMEMIKIEEPTLSMNFLVNDSPFVGKSGKFVTTRHLK
                     DRLEKELEVNVGLKVEPLDTTDGYKVSGRGELHLSILLENMRREGYEVGVSKPEVLMH
                     KEDGKLMEPIERVVVNCPEVYSGTIINELNMRKGMMESMSIEGDYVKIEFLAPTRGLL
                     GYRSEFINATRGEGTLVRSFEKFEEFKGEIPSRGNGVLIAQGPGVTMGYSLNALSDRA
                     VMFVDPGVEVYEGMIIGMNSRKDDMVVNPCKNKKMSNVRASGSDDAIKLSPPRIFTLE
                     EALEFIEDDELVEITPDSIRLRKRFLNEHDRLRYNKSRQGK"
     gene            3348..3590
                     /locus_tag="L3M60_003300"
     CDS             3348..3590
                     /locus_tag="L3M60_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227931.1"
                     /translation="MLEVTKIIIKYFASESDTFVKEEVCNFEELLKWSHKFIEGKKIE
                     DVLYIKSKENYKVNVIKSLEIKELEIVRKQQIPPYM"
     gene            3738..3929
                     /locus_tag="L3M60_003301"
     CDS             3738..3929
                     /locus_tag="L3M60_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HBN6227932.1"
                     /translation="MIRVCPNCSNVDVNKLKTLVGEENVKTGCIGQCRAFKKEAVGII
                     DNELEIKETEAEFFEACKE"
BASE COUNT         1464 a          539 c          650 g         1380 t
ORIGIN      
        1 acaaaaggag gtattaaata tgggaatagt acttataaca gcagcattag ttttggttat
       61 gtatggactt tcaattttat cgtatatatc agaaagcaat atattagaag acactgtaaa
      121 tgataaccaa taaggttttg aattatataa ataagtgaga actgtaaata aaagttaatt
      181 tttatttgca gtttttttat atatatgtaa aataaagtta attaataaaa aataatgttt
      241 tatttatagt atatatggat aataatagta aagtataaga gtaatgagaa taatatataa
      301 gagaggtaaa cgttatggta gatatcattg taattggtgc tggaccagca ggtttaactt
      361 cagcaattta tgctatgaga gcagggctaa gtgttacagt atttgaaaaa aatatttatg
      421 ggggtcaagt tgcaagtacc tctgaggtag aaaattaccc agcagttcag aagatttctg
      481 gggtagaatt ttctaataat atatacaatc aagcagttgc tcaaggtgtt gatattcagt
      541 ttgatgaggt tgaagaaata aatctagagg gaaaaataaa gattgtaaaa acttcttcag
      601 ctgaacataa agcaaaggct gttattcttg caaatggagt tgaaagacgt aaattaggtt
      661 gtgtagggga acaagaattt acaggtagag gagtatctta ctgtgcaaca tgtgatggag
      721 cattttttaa agataaagaa gttgctatag taggtggagg aaatactgca ttagaagatg
      781 ctttattttt agccaacaat tgcacaaagg tgtatttaat tcacagaaga gatagtttta
      841 gaggtgaaga agtgttggaa aaatctgtga aagcaagaaa gaatattgag atactttaca
      901 gtcatggtgt agaaaaaata gaaggagaaa agactgtatc gaaaatagag gtaaaaaact
      961 taaagactga agaaaaaaga actatagatg tcagtgggat ttttattgct ataggtctta
     1021 aacctaataa taaaatgttt gaaaatgttc tcgatttgga tgaaggtgga tatataattt
     1081 cagatgaatc ttgtacaaca tctgtggaag gtgtatatgt agcaggagat agtagaacta
     1141 agtttttacg tcaaataata acagcagcat cagatggagc tattgcagct gttcaagcag
     1201 ctaattatat aaatgttgaa tagtataaat ttcaaataaa aatagccata taaactgaaa
     1261 taatttttca gtattatatg gctattttgc taactagagg taaatgtatg ttttatttac
     1321 cttgtctaga tttattatat cttaatctat catgttcatt taagaatctt tttctaagtc
     1381 ttatagaatc aggtgtaatt tcaactaatt catcatcttc tataaattct agagcttctt
     1441 caagagtaaa tattctagga ggagataatt ttatagcatc atctgaacca gaagcacgta
     1501 cgttactcat tttcttgttt ttacaagggt tgactaccat atcatctttt ctagaattca
     1561 ttccaattat cataccttcg taaacttcta caccagggtc tacgaacatt acagctctat
     1621 cacttaaggc attaagagaa tatcccatag taacaccagg tccttgtgct attaaaacac
     1681 catttcctct tgaaggtatt tctcctttaa attcttcaaa tttttcaaat gaacgaacta
     1741 aagtaccttc tccacgagta gcatttataa attcacttcg atatcctaaa agaccacgag
     1801 taggtgctaa gaactctatt tttacatagt ctccttctat tgacatagac tccatcatac
     1861 cttttctcat gtttaattca tttataatag taccagagta aacttcagga cagtttacaa
     1921 caactctttc tattggttcc attagttttc catcttcttt atgcattaaa acttcaggct
     1981 tagaaacacc aacttcataa ccttcacgtc tcatattttc aagtaatata gataagtgaa
     2041 gctctccacg tccagaaact ttatatccat cagtagtatc aagaggttca actttaagac
     2101 caacatttac ttcaagttcc ttttcaagtc tatctttcaa atgtctagtt gtaacgaatt
     2161 ttccactttt tccaacaaat ggagagtcat ttactaagaa gttcatagaa agagtaggct
     2221 cttctatttt tatcatttcc ataggtagtg gactatctaa atcgcatata gtctcaccta
     2281 tagagatatc aggtatacca gctataacaa ctatttctcc actagtagct tcatcaacct
     2341 caacttgttt taaaccttca tacacagaaa gttttgatac ttttccctta gatacaacag
     2401 aatcttctct acatatagca acttgtgtat tatttttaag agtacctttg taaattctac
     2461 ctataccaag tctacctaca taatcatcat atgcaagagc tgatatttgg aattgtagag
     2521 gttcattatc gtaatcagga taagcttcaa cgtgatttac aatagttttg aatagaggag
     2581 aaagttcttc gctatcatca tccatttcca gtttagcaat accttgttta gcaataccat
     2641 atattatagg aaattcgcat tgctcatctg ttgcatttaa gtctacaaat aaatcgaaaa
     2701 cttcattaac tacttcttcc gctctttggt cttttttatc aatcttgttt ataaataata
     2761 ttggttttaa accgaactct aaagattttt gtaaaacgaa tctagtttga ggcattgggc
     2821 cttcactagc atcaactaat agaataactg tgtcaacagt tttcataaca cgttcaactt
     2881 cagaagaaaa gtcactgtgt cctggagtat caactatgtt tattttgtaa tcctcatagt
     2941 ttattgcaca gttttttgaa tatatagtta tgcctctttc tttttcaagg tcgttactat
     3001 ccataacgca gtctttaact acttcgttct ttctaaaaac accactttga gaaaggaaag
     3061 catcaaccaa tgttgatttt ccagcgtcaa cgtgggctat aactgcgata tttataattt
     3121 tatgtttttg tgacattttt gttgctccct tcgattaaaa taacttatta attatagcac
     3181 aatatgttat taggtacaag gaggaatata ttaaattgta ttaatatagt tcaaattgga
     3241 taaaaatgtt agttttatat ccgaaaatta ttctgtttaa aaaaattttt tagacaaata
     3301 ataaccttaa tagatgaaaa gattaataaa agagtaaagg atgatatatg ttggaagtaa
     3361 ccaaaataat aattaaatat tttgcaagtg aaagtgatac ttttgtaaaa gaagaagttt
     3421 gcaattttga agaacttctt aaatggtctc ataaatttat tgaaggtaaa aagatagaag
     3481 atgttttgta tataaaatct aaagaaaact ataaagtaaa tgtaattaag tctttagaaa
     3541 ttaaggaatt ggaaatagtg agaaaacaac agataccacc gtacatgtag tattaataat
     3601 atataaaatt aatgtgtaat acttatatta ggataaatat ggtatgtatg gtaacaattt
     3661 atataaaaaa aacttttata aagtttatat ttgggtataa aagtattaat tagacattaa
     3721 ttaggaggaa cttaaatatg ataagagtat gtccaaactg ttcaaatgtg gatgtaaata
     3781 agctaaaaac tttggttgga gaagaaaatg taaaaactgg ttgtataggt cagtgtagag
     3841 cattcaaaaa agaagctgtt ggaataatag acaatgagct tgaaataaag gaaacagaag
     3901 cggagttttt tgaagcttgt aaagaataaa ttttaaataa aatataaatt ataataaata
     3961 gagcgtataa attgtataaa tattaaacaa tttatatgct ctatttttat gtatatattt
     4021 aattataagt ata
//
LOCUS       DAFNNB010000205         3990 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0210, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000205 DAFNNB010000000
VERSION     DAFNNB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0210"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            96..803
                     /locus_tag="L3M60_003302"
     CDS             96..803
                     /locus_tag="L3M60_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227933.1"
                     /translation="MLKVINLSKSYKNKKVVNSVSFDVNDGELFGFIGHNGAGKTTTI
                     KAIVGIHDFENGEVLINSKSIKKQPVECKKEMAYIPDNPDLYESLTGIQYLNFIADIF
                     EVAKDKREELIKYYSNKFELNKALGDLISSYSHGMKQKLAIISALIHSPKILILDEPF
                     VGLDPKASFILKEIMREFCDKGGCIFFSTHVLEVAEKICDKIAIIKDGNIIAYGTTEE
                     VKGSNSLESIFMELIEK"
     gene            850..2445
                     /locus_tag="L3M60_003303"
     CDS             850..2445
                     /locus_tag="L3M60_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227934.1"
                     /translation="MSNLSILIKTNIINEFKLNALKKSDGREKQKIMGMILSAIIITG
                     LLIFYVTTLSLSLVDILKQINQMEMLLIFGFGLSTLTTIVTSLYKTSSYLFQAKDLDI
                     LTSLPIKESTVLASKILMLVGANYLFSSICMIIPAVVYFLNSDVSITYFPYLILLFLA
                     LPLLPIVASSIIFMFIGQISSRIKYKNLVLIIGSIILMLLYTLLMSRLNSMSVEMLKN
                     SVSISETIRKIYPPIYYFVDALKTGSVVSFLAFIAISIIPFFMFVTLFAKKFKNINAK
                     MSEGYKSKTYKFKSQKTSRPSKALLKKEFKRYTSSFIYFLNSSASLLFLIVGAILILL
                     FGADKMGELLKLNIDFNFIKIQLLGVFFFIIVMNCTTYCSISLEGENLWILKSSPIDE
                     REIFWSKILMNFLLNAPLSILCLILVSIRLDFEIQFIIVSICLIVSFSMFVALMGLLS
                     NLLYPNLDWKNEVAVVKRSASMVITMLVSLAYVVILGGIYTLLGISFLNIYIILASML
                     TLILDFMIWRIISTKGVKIFKNL"
     gene            2548..3630
                     /locus_tag="L3M60_003304"
     CDS             2548..3630
                     /locus_tag="L3M60_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HBN6227935.1"
                     /translation="MRKLSKKEKDFIQYGFLLFLICITTYLVLTTLDIKMIPKIIRLV
                     NKTYIFLGILIIIIYMIIESIVTHLIINAVQKTKVKFIGIKMATMGFYYNLVTPFASG
                     SQPMQIYALTKYDVSLSKSVAIVTNKTVVFQSTVTVFCGILILLNKSLLIEQVHSVKV
                     LITFGMIMNVSMLLGGLLIVFSPKQVKMIAEVLINFLSKFKTFKFLDSKRDRINHYID
                     DYNYSIKMFIKNVRILVLSILLTVLQLIAYFSIVYCVYKASNLRGVSYIHIMTLQVFL
                     YMAISPIPTPGNVGANEVTFFTMFSSIIPKELLGYSVLLYSGFVYYFILIVSGIFTFG
                     THYTLNSWKKETKVNSNLDKVIEKLN"
     gene            3779..3940
                     /locus_tag="L3M60_003305"
     CDS             3779..3940
                     /locus_tag="L3M60_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III"
                     /protein_id="HBN6227936.1"
                     /translation="MKQDKKNEKQSEISKIKQSNDNKLEVAQELHVSKNIGNMLGYRI
                     DKKKMNRPS"
BASE COUNT         1549 a          387 c          592 g         1462 t
ORIGIN      
        1 aaatttttaa aataaacaaa taaaagttgt aattgtttga tgtttgttat atattatata
       61 taacaaatat caaaactata atatggaggg aaattatgtt aaaagtaatc aatttatcta
      121 aatcatataa aaataaaaaa gtagtaaata gtgtctcatt tgatgtaaat gatggagagc
      181 tttttgggtt tataggacat aatggagcag gtaaaactac tactattaaa gcaattgttg
      241 gaatacatga ttttgaaaat ggagaggtac taataaattc taaatctata aaaaaacagc
      301 ctgttgaatg taaaaaggaa atggcatata tacctgataa tccagattta tacgaatcat
      361 taactggtat acaatacctt aattttatag ctgacatatt tgaagttgcc aaagataaaa
      421 gagaagagtt aataaaatat tattcaaata agtttgaact caataaagca ttaggtgatt
      481 taatatcttc atattcgcat ggtatgaaac aaaaattagc tataatttcg gccttgatac
      541 atagccctaa aattttaatt cttgatgaac cttttgtagg tcttgaccca aaagcttcct
      601 ttatattgaa ggaaattatg agggagtttt gtgataaagg tggttgcata tttttctcaa
      661 cacacgtttt agaagttgct gaaaaaattt gtgataagat tgctataata aaagatggaa
      721 atataattgc ttatgggact acagaagaag ttaaaggaag taattcactt gaaagtattt
      781 tcatggaatt aatagaaaaa taaaatgttt tttatacaat atagtatcta aagtgaggaa
      841 tgataaaata tgagcaatct aagtatatta ataaaaacta atataataaa tgaatttaaa
      901 ttaaatgcat taaaaaaatc tgatggtaga gaaaaacaaa aaataatggg aatgatactt
      961 tcagctatta tcataacagg gttattaatt ttttatgtaa ccactttaag tctttcctta
     1021 gttgatattt taaagcaaat aaaccagatg gaaatgctac tgatatttgg atttggattg
     1081 tcaacattaa caactatcgt tacatcatta tataaaacgt catcatatct ttttcaagca
     1141 aaagatttag acattttgac tagtttacct ataaaagaat ctactgtact agcaagtaag
     1201 atattaatgt tggtgggagc aaactattta ttttcttcaa tctgtatgat tataccagca
     1261 gtggtttact ttttaaattc tgatgtaagt ataacgtatt tcccttattt gatattgcta
     1321 tttttagctt taccattatt accaatagtt gcttcatcaa taatttttat gtttatagga
     1381 cagatatcat caagaataaa atataaaaat ttagtattaa ttataggtag tattatattg
     1441 atgttgcttt atactctact tatgtcaaga cttaactcta tgtcagttga aatgttaaag
     1501 aatagtgtat ctatttcaga gacgattagg aaaatatatc cacctatata ttattttgtt
     1561 gatgctctta aaacaggaag tgtagtttcc tttttagcat ttatagcaat ttcaataata
     1621 ccatttttta tgtttgtaac tttatttgca aaaaaattta aaaatataaa tgctaagatg
     1681 agtgaaggat ataagtcaaa aacttacaaa tttaagagtc aaaaaacttc aagaccttca
     1741 aaagctctat taaaaaaaga atttaagaga tatacttctt catttatata ttttttaaat
     1801 tcatctgcaa gtttgttatt tctaatagta ggtgcaattc taatattgtt atttggagca
     1861 gataaaatgg gtgagttgct gaaattgaat atagatttta attttataaa aatacaatta
     1921 ttgggagtat tcttctttat aatagtaatg aattgtacaa catactgttc aatttctctt
     1981 gaaggagaaa atttatggat actgaaatct tctccaatag atgaaagaga aatattctgg
     2041 tcaaagatat tgatgaattt tttattgaat gcaccattat ctattttatg tttaatatta
     2101 gtatctataa gattagactt tgaaattcag tttattattg tatcaatttg tttgattgta
     2161 agtttttcaa tgtttgtagc attgatggga ttattatcaa atttactata tccaaacctt
     2221 gattggaaaa atgaagtagc tgtagtaaaa agaagtgcaa gtatggtaat tacaatgtta
     2281 gtatcattag cttatgttgt tatacttgga ggtatatata cattacttgg aatttcgttt
     2341 ttaaatatat atattatttt ggcaagtatg cttaccttga tattagattt tatgatatgg
     2401 agaattattt ctactaaagg agtaaaaata tttaaaaatc tataggtgta gttatgtcaa
     2461 ataaagactt atctttttta gtcttgcaaa taataggtta agtagtatat tatgtatagt
     2521 gatatgaaaa aatttaggag aaaataaatg agaaaattat ccaaaaaaga aaaagatttt
     2581 attcaatatg gatttttatt atttttgata tgtattacta cttatttagt attaactact
     2641 ttagatataa aaatgatacc taaaattata agactagtaa ataagactta tatatttctt
     2701 ggaatattga taattataat atatatgata attgaatcta ttgtaactca tttgataata
     2761 aatgcagttc aaaaaacaaa agtgaaattc attggtatta aaatggctac aatgggattt
     2821 tattataact tagttactcc atttgcatct gggagtcaac caatgcaaat atatgcactt
     2881 acaaaatatg atgttagtct tagtaaatca gtggctatag ttactaataa aacagtagta
     2941 tttcaaagca cagttaccgt attttgtgga attttaattt tgttaaataa gtcattactg
     3001 atagagcaag tacattctgt gaaagtcttg ataacttttg gaatgattat gaatgtaagt
     3061 atgttgttag gtggactact aatagtattt agtccaaaac aagtaaaaat gatagcagaa
     3121 gttttgatta attttttatc taaatttaaa acctttaaat ttttagattc aaaacgtgat
     3181 agaataaatc attatattga tgattataat tattctataa aaatgtttat taaaaatgta
     3241 agaatcttag ttttaagtat attacttact gttttacaat taatcgcata cttcagcata
     3301 gtttattgtg tgtataaagc ttcaaattta agaggagtct catatataca tataatgaca
     3361 ctacaggtct ttttatatat ggctatatcg ccaattccaa ccccaggaaa tgttggagct
     3421 aatgaggtta ccttttttac tatgttttct agtataattc caaaagaatt attggggtac
     3481 tccgtgctct tatatagtgg gtttgtgtac tattttatat taatagtttc aggtatattt
     3541 acatttggaa cgcattacac attaaattct tggaaaaagg aaactaaagt aaattctaat
     3601 ttagataagg ttattgaaaa attaaattaa taaaataatt agggaaattc tatatataaa
     3661 tatggtgtta gataagctat attatattga gatatttccc ttttttgatt aaaattatag
     3721 aaatttgaaa taataagtaa taaacagaac gattgatatt gttttgaaag gagaagttat
     3781 gaaacaagat aaaaaaaatg aaaaacaaag tgagatttct aagataaaac aaagtaatga
     3841 taataaactt gaagtagctc aagaattaca tgtaagtaaa aatataggaa atatgcttgg
     3901 atatagaata gataagaaaa aaatgaatag accaagttaa tgaaaatgtt gaaaatagta
     3961 cattttaaaa aaaatatata atgaaatgtt
//
LOCUS       DAFNNB010000206         3958 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0211, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000206 DAFNNB010000000
VERSION     DAFNNB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3958
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0211"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            473..1057
                     /locus_tag="L3M60_003306"
     CDS             473..1057
                     /locus_tag="L3M60_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227937.1"
                     /translation="MNKDIGAKIKQLRTQKQMTLKDMSEKTNLSIGFLSQLERGLTSV
                     ATDSLGKIASVLDVELTYFFMKPKEHKRAVLRSYEKEVFDVENSTFIHYHLSSSLKEK
                     TMLPRLIEILPSKSSEEICCYVHEGEEFVYVLEGTLTVFLGDEQIEMYPGDTIHYNSE
                     KNNHNWVNYTNKVAKILVVSVPNPFEKSNEVKEA"
     gene            1097..1900
                     /gene="proC"
                     /locus_tag="L3M60_003307"
     CDS             1097..1900
                     /gene="proC"
                     /locus_tag="L3M60_003307"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBN6227938.1"
                     /translation="MKKIGFIGAGNMASAMIGGIVNSKLVEPNMVIASAYSQETLDRI
                     YANFGINTTKDSKEVTRSSDIVIVAVKPDIYDDILEEIKDFIDDNKIIVTIAAGKSIK
                     DIESIVGDDKKIVRTMPNTPALVNEAMSSLSINKNINDEDLEAVTEVFNSFGNTEVVP
                     EYLIEAVIGASGSAPAYVFLFIEAIADAAVIAGMPRPQAYKFASQAVMGSAKMVLETG
                     KHPGELKDMVCSPGGTTIEAVKVLEEEGFRASVIKAICACIEKSKKMSE"
     gene            complement(1981..3156)
                     /locus_tag="L3M60_003308"
     CDS             complement(1981..3156)
                     /locus_tag="L3M60_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HBN6227939.1"
                     /translation="MKFKKQISSIVVSTMLVLGSMNLSYADGTNVVTLGANLSESQKQ
                     QMLNYFGVKKDQVLVLDVTNAEERQYLQGVATEGQLGKVTISCSYVEPTKKGNGINVK
                     TANLTWVTSSMIASTLTTAGLEDANVIAAAPYPVSGTGALTGIMKAFEDASGKKLDET
                     KKEIASEELVVTGDLGDDIGQEKATGVMNDIKTEIVKNGTKDTNQIANTINNVVNNYN
                     ITITPEQKEQIKGVMKKIADQDYDYNSMKNTLDNVSDNVNEQLKKLGESVKSSGILDT
                     IGGWFSGIGDWFSGLFSGGDKKDLGILNNTNDESLGSDAVINSTEGVQINPQNNTNSN
                     SDSDTNNSKNNTSTNNENKDNSQTNENDTTNNSENQSNNNDSVGFFEKIKNWLSGLF"
     gene            3422..3898
                     /locus_tag="L3M60_003309"
     CDS             3422..3898
                     /locus_tag="L3M60_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HBN6227940.1"
                     /translation="MSKELVKDRVIKYLIEDLLVPQDMIDTNVELAEFEEGAEGILDI
                     VVNVKDEEDYYAPVMIVQCLDEDVELEGEVLQKQIEFLEEVDNITMSGRLVLTNGDAM
                     MYADWRGEEYDTEAALPTYDIMVKEFHEMEQQVKDLEEHHHHDENCGCGCNHHHEN"
BASE COUNT         1479 a          480 c          653 g         1346 t
ORIGIN      
        1 tacttcaata attcttgaca atctaaaaat tgacaagact gtatataaaa aatatatata
       61 gtgctaaact ctaaaataaa atatgttgta gagtaaaaaa agacattgaa tagatatcat
      121 taaattgtaa aattatgtta taattgtact aaaggatgta ataatgtgta gaaataataa
      181 tataatttga aattttaata actaaagcta aagaaaattg tatgaataac ttaagtttta
      241 tatataaaga gtagatgctt ataaaaaagg tatatttagt tggcgaaatt aaatatacat
      301 gtgtgaaaaa taaatcgtaa ataatcgtaa aaatgtagac aaaaaaagat taataaatta
      361 tagtaatact tataaaattt tttaaaaatg aaataaaggt aggtttaata cctatattta
      421 tatttttgta tttatattat gaaaaaatta aaattattta ggggtgatgg ttttgaataa
      481 ggacatagga gcaaaaataa agcaactcag aactcaaaag caaatgacac tgaaggatat
      541 gagtgaaaaa acaaacttgt caattggttt tttatcacaa cttgaaagag gattaacaag
      601 tgtggcaaca gattccttag gaaaaatagc aagtgtacta gatgtagaat taacttactt
      661 ttttatgaag ccaaaagagc ataaaagagc tgtattaaga agttatgaaa aagaagtatt
      721 tgatgtagaa aactcaacat ttatacatta tcatttatca tctagtttaa aggaaaaaac
      781 aatgttaccg agattaattg aaattcttcc aagtaagagt agtgaggaaa tttgttgtta
      841 tgtacatgaa ggtgaggagt ttgtgtatgt attagagggc actcttacag tgtttttggg
      901 agatgagcaa atagaaatgt atccaggaga cacaatacac tataatagtg aaaagaacaa
      961 tcataattgg gttaactata ctaataaggt agctaaaatt ttggtagtaa gtgtaccaaa
     1021 tccatttgaa aaatctaatg aagtaaaaga agcttaaatt ttagcaacta ctattagcta
     1081 ataaggaggt actaaagtga aaaaaattgg attcataggt gcaggaaata tggcaagcgc
     1141 tatgataggg ggaatagtaa attcaaaatt agttgaacct aatatggtta tagcatcagc
     1201 atatagtcaa gaaactcttg atagaatata tgcaaatttt ggaataaaca ctacaaaaga
     1261 ttcaaaggaa gtaactagaa gctctgatat agtaattgtg gctgttaaac cagatatata
     1321 tgatgatatt ttggaagaga taaaagattt tatagatgac aataaaatta tagttacaat
     1381 agcagcagga aaatctataa aggatataga gtctattgta ggagatgata aaaaaatagt
     1441 tagaactatg ccaaatactc cagctcttgt aaatgaagcc atgtcttctc tttctataaa
     1501 taaaaatata aacgatgaag acttagaagc agttacagaa gtatttaatt cttttggaaa
     1561 tactgaggta gttccagagt atcttataga agctgtaatt ggtgcaagtg gttctgcacc
     1621 agcatatgtg tttttattta tagaagctat agcagatgca gcagtaattg caggaatgcc
     1681 aagaccacaa gcatataagt ttgcatcaca ggctgttatg ggctctgcaa aaatggtact
     1741 tgagacaggg aaacatcctg gtgagttaaa agatatggta tgttcacctg gtggaactac
     1801 tatagaggca gttaaagtac ttgaagaaga aggttttaga gcttctgtga taaaagcgat
     1861 atgtgcatgt atagaaaaat ctaaaaaaat gagtgagtaa taaaaattta ttgtaaataa
     1921 agagaattgc cttttataga aaatttccat tttaggcaat tctttttatt tgtttaattt
     1981 ttaaaatagg cctgaaagcc aattttttat tttttcaaag aaaccaactg agtcattatt
     2041 atttgattga ttttctgaat tatttgttgt gtcgttttca tttgtttgag aattatcttt
     2101 attttcatta ttagttgaag tattattctt tgagttattt gtatcactgt cactgttaga
     2161 attagtatta ttttgtgggt ttatttgaac accttctgta gaatttatta cagcatcaga
     2221 gccaagagac tcatcattag tgttatttaa gattcctaaa tcttttttat ctccacctga
     2281 gaataaacca gagaaccagt ctcctattcc actaaaccat ccaccaatag tatctagtat
     2341 accagaactc ttaacacttt cacctaattt ctttaattgt tcatttacat tatctgatac
     2401 gttatctagt gtatttttca tgctattata atcgtaatct tggtcagcta tttttttcat
     2461 tacaccttta atttgttctt tttgttctgg tgtaatagta atattgtaat tatttacaac
     2521 attatttatt gtatttgcta tttgattggt atcttttgta ccattcttta caatttctgt
     2581 ttttatatca ttcattacac ctgttgcttt ttcttgccct atatcgtctc ctaaatcacc
     2641 tgtaaccacc aattcttcag aagcaatttc tttcttcgtc tcatcaagct tctttcctga
     2701 agcatcctca aatgctttca tgattcctgt caatgctcca gtaccactta caggatatgg
     2761 agcagcagca ataacatttg catcttcaag accagctgtt gtaagtgttg aagcaatcat
     2821 tgagctagtt acccaagtta agtttgctgt tttaacatta attccatttc cttttttagt
     2881 tggttcaaca tatgaacaag atatagtaac ttttccaagt tgaccctctg ttgcaacacc
     2941 ttgtaaatat tgtctttctt ctgcatttgt tacatctaaa actaagactt ggtctttttt
     3001 tactccaaag taattaagca tttgctgctt ctgagactct gataagttag ctccaagtgt
     3061 aactacattt gtaccatctg cataagaaag attcatactt ccaagtacaa gcatagtaga
     3121 cacaacaata gaactaattt gttttttaaa cttcataata ttcctcctag atttaacata
     3181 cattacattt tcaatctatt ttttattaat atattgaata aaaatatgtg ttacatttct
     3241 attatactaa atataaacta ttttttaaat caatgcgatt acaactttgt aataaaaaag
     3301 gaaatataag gaagataaca aaaaaataat ttttgtaaga gattgaaaaa acaattaatg
     3361 tagtatacta taatatagaa atgattatga taatgttata gaatcggaag gagaaaaata
     3421 aatgtcaaaa gaattagtaa aagatagagt aataaaatat ttaatagaag acttattagt
     3481 acctcaagat atgattgata caaatgttga gttggcagag tttgaagaag gtgcagaagg
     3541 aattttagat attgttgtaa atgttaaaga tgaagaagat tattatgcac ctgtaatgat
     3601 agtacaatgt ttagatgaag atgtggagtt agaaggagaa gttttacaaa aacaaataga
     3661 atttttagaa gaagtggaca atataactat gtcaggtaga ttggttttaa ctaatggaga
     3721 tgctatgatg tacgctgatt ggagaggtga agaatacgat actgaggcag ctcttccaac
     3781 ttatgatata atggtaaaag agtttcatga aatggaacaa caggtaaaag atttagaaga
     3841 acatcatcac catgatgaaa attgtggatg tggatgtaat catcatcatg aaaattaaat
     3901 attgagcata tatataaaaa aagactgttt agtaaacagt ctttttttag acatatat
//
LOCUS       DAFNNB010000207         3948 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0212, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000207 DAFNNB010000000
VERSION     DAFNNB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0212"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..945
                     /locus_tag="L3M60_003310"
     CDS             <1..945
                     /locus_tag="L3M60_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HBN6227941.1"
                     /translation="VVSNEIEDINGHGTSCASIIKSEYEYVDFIIIKIFDRDGLTNIK
                     VLEESLNYLISIDVKIINLSLAIHGDINSCKDLYKICEKLKNQNKIIVSSLANYNYIE
                     ESYPSIFDNVIGVKGNLLKKRSSFWYNSEYKYQCVVDDSDRIVADLGNSFKLVEKSNS
                     FSAAKMSGKIAKIIANKVDISFDGLSRILEELSECTNWKDEDLYTNKRRIDYKQYLYE
                     NNNINLNRVLKVLVDNLNIGCNVDILFDSILFSKYIGLKNNNCIEILKKLENEFNIKI
                     DYTKVYKEDLISIYTLTNLIEKESICEHFRNCKYLQKL"
     gene            905..1570
                     /locus_tag="L3M60_003311"
     CDS             905..1570
                     /locus_tag="L3M60_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227942.1"
                     /translation="MNILETVNISKSYSIGDKKSYILKDINIKIKEGEFISIIGPSGS
                     GKSTLLHILGGLENPTTGIVKIIGKSIYDLSEIKLAEYRRRRFGFIFQQFNLIPVLNV
                     RENIEMPLMLDRAKIDNRFIKEIIEFLGLEEKENYYPNQLSGGQQQRVAIARALVSKP
                     DIIFADEPTGNLDTKNSDEVIKLLMKSIHKYNQTVLLITHDYKIAKNTDRTIKILDGK
                     LEL"
     gene            1571..3346
                     /locus_tag="L3M60_003312"
     CDS             1571..3346
                     /locus_tag="L3M60_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227943.1"
                     /translation="MIQCQEGNKLKNTINMKKFLYLIIQGNKKLVIISFLMMIVVAMI
                     DLYIPQIIRNILDIAIKSKNITMLIKLILQFILINIILSNLELFLEYNYSKMKKKITT
                     KLKLKLLRHLSKLDGRFFSEIKTGNIMNIVEYDVMMLDRFGIELIFSLIVDIITAFIS
                     LYFLIKIQSKLLVIVVVIQIVISFTQYIFTKMISKYTQNIRSNAGSISNIVQEYISNI
                     MNVVIHKGELNFFTQFLSMQKNFVSKCIKLDMIMAGNVSLSKILNSLIVISIYGYGGY
                     KIINGEITFGELVAFQQYTGMLIGPCMNIIKSNAIVQQSLVSINRIFDLLNEEVNMKQ
                     ENTGEIFQSGITKKIRMDMIDVSFSYDKEDVLHNINMSLESGNIYGIVGGSGSGKSTL
                     IKLIYRLWDIESGSIRINDVDIRDYNLKNLRKNITIITQEPLLLNDTVFNNIILSNKN
                     INYELVVRICKELDIYDFIVSLPNEFNTIVGENGVKLSGGQKQRISIARAMLSNSSIL
                     VLDEATSALDNISQKDLLKSMKKFLNNKIVIIIAHRLSTIKDSDKIFVINNGEIIESG
                     SHKDLVSQNGYYCNLLEDKKEYSYC"
     gene            3516..>3948
                     /locus_tag="L3M60_003313"
     CDS             3516..>3948
                     /locus_tag="L3M60_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6227944.1"
                     /translation="MRLSLAYVKKYFGRSISIALSIIISISLIITVGILTKNTQKASV
                     DRIKYESGDYHVKVKNISGNVISDYKKQFDISMMGVSSYYDSSEFNGKLMLNLIKSNE
                     DYIKIYNSKILNGRFPTSDNEVALEEWVMLNLGLKLDINQNI"
BASE COUNT         1671 a          331 c          616 g         1330 t
ORIGIN      
        1 gttgtctcaa atgaaattga agatattaat ggacatggta catcatgtgc atcaattata
       61 aaaagcgaat atgaatatgt tgattttata ataattaaaa tatttgatag agatggatta
      121 acaaatatca aagtactaga agaatcatta aattacttaa taagtattga tgtaaaaatt
      181 ataaacttga gtttagcaat acatggagac attaattctt gtaaagactt atataaaata
      241 tgtgaaaaac taaagaatca aaataaaatt atagtttctt cccttgcaaa ttataattat
      301 atagaagaga gctatccatc tatatttgat aatgtaatag gagtaaaagg aaatttatta
      361 aaaaaaagat catcattttg gtataattca gaatataaat atcaatgtgt tgtagacgat
      421 agtgatagaa tagttgcaga tttaggtaac agttttaagt tagttgaaaa aagcaacagt
      481 ttttcggctg ctaaaatgag tggaaaaata gctaagataa tagcaaataa agtggatata
      541 agttttgatg gattaagtag aatactagaa gaattgtctg agtgtacaaa ttggaaagac
      601 gaagatctat atactaataa gagaagaata gattacaaac agtatttata tgaaaacaat
      661 aatataaatc ttaaccgagt attaaaagtg ttggttgata atcttaatat tgggtgtaat
      721 gtggatattt tatttgatag tatattattt agtaaatata taggtctaaa aaataataat
      781 tgtattgaaa tcctaaagaa actagagaat gaatttaata taaagattga ttacacaaaa
      841 gtatataaag aagatctaat atcaatatat actttaacta atttgattga aaaggagagt
      901 atatgtgaac attttagaaa ctgtaaatat ctccaaaagc tatagtatag gcgataaaaa
      961 atcatatata ttaaaagata taaatataaa aataaaagaa ggagaattta tatctataat
     1021 aggtcctagt ggaagtggaa agtcaacttt acttcacata ttagggggat tagaaaatcc
     1081 tactactggt attgtaaaaa ttataggaaa aagtatatat gatttatctg agattaaatt
     1141 ggcagaatat agaagaagaa ggtttggatt tatatttcaa caatttaatc taataccagt
     1201 acttaatgta agagaaaata ttgaaatgcc attaatgcta gatagagcta aaattgataa
     1261 taggttcata aaagaaataa ttgagttttt aggtttagaa gaaaaagaaa attactatcc
     1321 gaatcaatta tctggagggc aacaacaaag agtagctata gctagagctt tagtaagtaa
     1381 gccagatatt atttttgcag atgagcctac tggaaaccta gacactaaaa atagtgatga
     1441 ggttataaaa cttttaatga agtctataca caagtataat cagactgtat tattaattac
     1501 tcatgattat aaaattgcta aaaacacaga tagaacaatt aaaatactag atgggaaact
     1561 agaattataa atgatacaat gtcaagaggg taataaattg aaaaatacta ttaatatgaa
     1621 aaagtttcta tatctcataa ttcaaggtaa taaaaagcta gtaattatat cttttcttat
     1681 gatgatagta gtagcaatga tagatttgta tatacctcaa ataataagga atatattgga
     1741 tatagctata aaatcaaaaa atattactat gctaataaaa ctaatattac aatttatatt
     1801 aattaacata atattatcta atctagaact atttctggaa tataattatt ctaaaatgaa
     1861 gaagaaaata actacaaaat tgaagttaaa attattgaga catttatcaa agttagacgg
     1921 acgttttttc tctgaaataa aaacaggaaa tattatgaac atagtagagt atgatgtaat
     1981 gatgttagac agatttggaa tagaattaat attttcatta attgtggaca taataactgc
     2041 atttatatcg ttgtattttt taataaagat acaatcaaaa cttttagtaa tagttgtagt
     2101 aattcaaatt gtaatttctt ttactcaata tatatttaca aagatgattt ctaagtatac
     2161 acagaatata agaagtaatg ctggaagtat atctaatata gtacaagaat acatatctaa
     2221 tatcatgaat gttgttatac ataaaggtga acttaatttt tttacgcaat ttctatcaat
     2281 gcaaaagaat tttgtaagta aatgcataaa gctagatatg attatggctg gaaatgtttc
     2341 tttatctaag atattaaaca gtttaattgt tatttctata tatggttatg gtggatataa
     2401 aattataaat ggagaaatta cgtttggaga attggtagct tttcaacaat atacaggtat
     2461 gcttattggc ccttgtatga atataataaa atctaatgct attgtacaac aatcattagt
     2521 atctattaat agaatatttg atttattaaa tgaagaggtt aatatgaagc aagaaaatac
     2581 aggtgaaata tttcagagtg ggataactaa aaaaattaga atggatatga tagatgtaag
     2641 tttttcatat gataaagaag acgtattaca taatataaat atgtcactgg agagtggtaa
     2701 tatctatgga attgttggag gaagtggttc aggaaaaagt actttaatta aactaatata
     2761 tagattatgg gatattgaaa gcggaagtat aagaattaat gatgtagata taagagatta
     2821 taatttgaaa aatttaagaa aaaatattac tattatcaca caagaaccat tattattgaa
     2881 tgatactgtt tttaataata taattttaag caacaagaat attaactatg agctagtagt
     2941 aagaatatgc aaggaattag atatatatga ttttatagta agtttaccca atgaatttaa
     3001 tacaattgta ggagaaaatg gtgtaaaatt gtctggtggg caaaaacaaa gaatttccat
     3061 agctagagca atgttgagta attcaagtat tctagttctt gatgaggcta catctgctct
     3121 tgataatata tctcaaaaag atttattaaa aagtatgaaa aagttcttaa ataataaaat
     3181 agttataata atagctcata gactatctac tataaaagat tcagataaaa tatttgtaat
     3241 taataatggt gaaataattg aatctggaag tcataaagat ttagtgtctc aaaatggata
     3301 ttattgtaat ttacttgaag ataaaaaaga atatagttac tgttgatgat tagatagtca
     3361 ataaaaattc tatttttata ttagaatata ttgactattt ttttgtatta taaatacaat
     3421 gtatgaatga ataagtacaa ggtatgtaac atttattgat atatatttat taaaatgata
     3481 catttatttc agtaattaca taaaggagga attttatgag actatctcta gcatatgtaa
     3541 aaaaatactt tggaaggtca atatctatag ctttaagcat aatcattagt atctcactta
     3601 taattacagt tggaatactt actaaaaata ctcaaaaagc aagtgttgat agaataaagt
     3661 atgaatctgg agattaccat gttaaagtta agaatattag tggaaatgta atatcggatt
     3721 ataaaaagca atttgatata agtatgatgg gtgtatcatc ttattacgat tcaagtgaat
     3781 ttaatggaaa attaatgtta aatttaatta aatctaatga agactatata aaaatttata
     3841 attcaaaaat tctaaatgga agatttccaa ctagtgacaa tgaagttgct ttagaagaat
     3901 gggttatgtt gaatttaggt ctaaaattag atataaatca aaatataa
//
LOCUS       DAFNNB010000208         3947 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0213, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000208 DAFNNB010000000
VERSION     DAFNNB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0213"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            205..2562
                     /locus_tag="L3M60_003314"
     CDS             205..2562
                     /locus_tag="L3M60_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HBN6227945.1"
                     /translation="MGNLNLKRYNCDIKMGLSSEQIQSRIDNNLVNVFDNGKTKTYKQ
                     IFKDNVFTLFNLINIILVCLLVSVGSFKNTLFIFIAVINTIIGVIQEIRAKRLLDNLS
                     VIVSNKVSVIRDKEKKEIDVQELVLDDIMILKTGNQICADSKVLDGRLEVNESLITGE
                     SDIVIKNKGDFLYSGSFVVSGEAFVRVENIGKDNYANKIANDAKISKKHNSQLRNSLN
                     AILKVVGIIIIPLGLILFAKQHFGNGDSIVTSIVGTVAAVNGMIPEGLTLLTSIALAL
                     GTIKLASHKTLVQELYCVETLARVDTLCLDKTGTITEGKMQVDDVVMLEELDIDDIMG
                     NICNALKDDNATLNAIRDKYSILDTHKVKNLIPFSSERKYSGVIFEGEGTYLLGAFEF
                     IFKDKYKELKTEVEKYSKKGNRVIVLAHSNSYMEDNNIPEDIKPLAFILILDKIRDEA
                     KETLEFFYNEGVDIKIISGDNPMTVSEVARKAGLKTAGNFIDATTLKNDEDIYEAVDK
                     YNVFGRVSPQQKKQMILALKKQNHTVAMTGDGVNDVLALKEADCSIAMASGSDVTKNV
                     SNLVLLDSNFASMPKVVMEGRKVINNIQRTASLFLVKTIFSFFLSILTLFFFSRQYPF
                     VPIQLALIGAVTVGIPSFFLALEANRGRISGNFLLNILKNALPGALCVTINVIIVYNI
                     VEYLGYNSSVFSTMCAILTGVCGLLILRQQCLPFNKERIFLIVSMTIAFIVGILFLGG
                     LFSFVALSKQLILITVVLAVLMPIMIKVMRFVLDEILGAIVPNLN"
     gene            complement(2647..2964)
                     /locus_tag="L3M60_003315"
     CDS             complement(2647..2964)
                     /locus_tag="L3M60_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBN6227946.1"
                     /translation="MNEFKNVTAVKKANVYFDGKVSSRVIILPNGERKTLGLMLPGEY
                     TFSTREEEIMEILAGSMDVKLPGSNEFVTYKEGQKFNVPSDSSFDLKVNEVVDYCCSY
                     IAD"
     gene            complement(3496..>3947)
                     /locus_tag="L3M60_003316"
     CDS             complement(3496..>3947)
                     /locus_tag="L3M60_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBN6227947.1"
                     /translation="ENNVMAVHCPASNFNVGSGAMPIRKLIDNNIRLALGSDISGGHT
                     LSIFKAMVSAIQLSKLYWVNSGKKYNFLSLSEAFYIATKSGGSFFGKVGSFEEGYDFD
                     ALIIDDSDLNHDNYSILERLERFIYVGDDRNIIHRYVCGKLIEEPNI"
BASE COUNT         1531 a          450 c          588 g         1378 t
ORIGIN      
        1 atttactttt taatttaaga aaaaattaac taaaatatat cgaaatttat aaaaatttag
       61 taaatcttaa gaattataat atttaaatct ttagatttat attttattat ataaaatata
      121 gaagaaaaaa ataaaaaaaa tgataatttt ataatatatt tataatttta agtataaaaa
      181 ttgttaaatt aaaaggagca gagtatgggt aatcttaatt taaaaaggta taattgtgat
      241 ataaaaatgg gattaagtag tgaacaaatt caaagtagaa tagataataa tttagttaat
      301 gtatttgata acggaaaaac taaaacgtat aaacaaattt ttaaggataa tgtgtttaca
      361 ctttttaatc taataaatat tattttagta tgtttactag tttcagttgg ttcttttaaa
      421 aataccttat ttatatttat tgctgtaatt aatactatta taggcgtaat tcaagaaata
      481 agagctaaaa gattgctcga caatctatct gttatagttt caaataaggt atctgttata
      541 agagacaaag aaaaaaaaga gattgatgta caggaattgg tactagatga tattatgatt
      601 ttaaaaacag gaaatcaaat ttgtgcagat tcaaaagttc tagatggaag attagaagta
      661 aatgagtctt taattacagg agaatctgat attgtcataa aaaacaaagg tgatttctta
      721 tattctggta gctttgtagt ttctggagaa gcttttgtaa gagttgaaaa tataggaaaa
      781 gataactatg cgaataagat agctaatgat gccaagattt ctaaaaaaca taattctcag
      841 cttagaaatt cattaaatgc aatattaaaa gttgtaggta taataattat tcccctaggg
      901 cttatattat ttgcaaagca acattttgga aatggagatt ctatagttac ctctatagta
      961 ggaactgtgg cagctgtaaa tggaatgata ccagaaggac tgacactact tacaagtatt
     1021 gcactggctt taggaactat aaagcttgca agtcataaaa cgttagtaca agaattatac
     1081 tgtgtagaaa ctcttgctag ggttgacaca ttatgccttg ataaaacagg cactattaca
     1141 gagggtaaaa tgcaagttga tgatgtagta atgttagagg agttagatat tgatgatata
     1201 atgggaaata tatgtaatgc tcttaaggat gataatgcaa cactaaatgc gataagagat
     1261 aaatacagta ttttagatac acataaagtt aaaaatttaa ttcctttttc atcagaaaga
     1321 aaatatagtg gtgtcatatt tgaaggtgaa ggaacatatt tactaggagc atttgagttt
     1381 atttttaagg ataaatataa agagcttaaa acagaagtag aaaaatattc taaaaaaggc
     1441 aatagggtaa tagttttagc tcatagtaat tcttatatgg aagacaacaa tattcctgaa
     1501 gacataaaac cactagcatt tatattaata ttggataaaa ttagagatga agctaaggaa
     1561 acattagaat ttttttataa tgaaggtgta gatataaaaa ttatatctgg agacaatcca
     1621 atgacagtaa gtgaagtggc tagaaaagca ggtcttaaaa cggctggtaa ttttattgat
     1681 gcaacaacac ttaaaaatga tgaagatata tatgaagcag ttgataaata taatgttttt
     1741 ggaagggtaa gcccacagca gaaaaaacaa atgattttag cattgaaaaa acaaaatcat
     1801 acagtagcta tgactggaga tggtgtaaat gatgtgctag cacttaagga agctgattgt
     1861 agtatcgcta tggcttctgg aagtgatgtt actaaaaatg tttctaattt ggtattgttg
     1921 gattcaaatt ttgcatcaat gccaaaggtt gttatggaag gaagaaaggt aattaataat
     1981 attcaaagaa cagcttcttt atttttagta aagacaattt tctcattttt cttgtcgata
     2041 ttaacattat ttttctttag tagacaatat ccatttgtgc ctatacaact agctctaatt
     2101 ggcgctgtaa cagtgggtat accatcattc ttcttagctc ttgaagcaaa tagaggtaga
     2161 atttcaggta actttttact caacatatta aaaaatgctt taccaggagc actttgtgtg
     2221 acaataaacg taattattgt ttacaatata gttgaatact taggttataa ttcaagtgta
     2281 ttttctacaa tgtgtgctat attaactggt gtgtgtggac ttttaatatt aagacaacaa
     2341 tgtttaccat ttaataaaga aagaatattt ttaattgtaa gtatgactat tgcatttatt
     2401 gttgggattt tatttttagg tggattattc tcatttgtag cattgagtaa acaattaatt
     2461 ctcataactg tggtactagc agtattaatg ccaataatga taaaagtaat gagattcgta
     2521 ttagatgaga tactaggtgc aatagttcca aatttaaatt agatttaata atatataaaa
     2581 agagcagtct tcattgaact gctcttttta gatgtgttta attttatgta agaatatgta
     2641 gctttattaa tcagctatat atgagcaaca gtagtcaacg acttcattaa cttttaaatc
     2701 aaaacttgaa tcactaggaa cattaaattt ttgaccttct ttataagtta caaactcatt
     2761 gcttccaggc aattttacat ccatacttcc agcaagtatt tccattattt cttcttctct
     2821 agtagaaaaa gtatattctc ctggcaacat taatcctaaa gtttttctct ctccattagg
     2881 tagtattatt actctactgc ttactttacc atcaaaataa acgtttgcct tttttactgc
     2941 agtaacattc ttaaattcat tcattagata aaacctccaa aattatttgt atatatacat
     3001 tatataaaaa taatataaaa aatgctatat atttacaaat aaatttagta tttgttaaaa
     3061 aaattaataa aaataaaaaa tgagtaaaat tacttaaatt gataaaaata tgtaaaataa
     3121 attaataatt gtaaaagaga ggagttttat ggcacatatg ttttgatgat atttattatt
     3181 tattaaaaga aacaatgagg ttaaaattaa tatcatataa taaaattgta ttactttaaa
     3241 acgatttatc aataggtaaa atttatgagg tatgaataca ataacaatat agttttacga
     3301 ttgtagatat caaaaaatgt atgttactag aaaggcgttt tgtagattta tatatgatgt
     3361 aatcattaaa gctataaata aactaaataa aaagagtatg ttttaaccta cgcttttaga
     3421 tttgaaatat actcttttat taattatagt tatacaaatt aaccagatta tatataatta
     3481 aaatttttat aataattata tattaggttc ttcaattagt tttccacata catatctatg
     3541 aatgatattt ctatcatcac caacatagat aaatctttca agtctttcaa gtatagaata
     3601 attgtcatga tttaaatctg aatcatcaat aatcaatgca tcaaaatcat aaccttcctc
     3661 aaagctacca acttttccaa agaaacttcc accacttttg gttgcaatat aaaaagcttc
     3721 agataaagac aagaaattgt acttcttacc agagttaacc caatataatt tagataattg
     3781 tattgcagat accattgctt taaatattga caatgtatgc ccaccactta tatctgaacc
     3841 aagtgctaat ctaatattat tatcgattaa ttttctgata ggcatagcac cactaccaac
     3901 attaaagtta gaagctggac agtgaactgc cataacattg ttttctc
//
LOCUS       DAFNNB010000209         3918 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0214, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000209 DAFNNB010000000
VERSION     DAFNNB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0214"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1327
                     /locus_tag="L3M60_003317"
     CDS             <1..1327
                     /locus_tag="L3M60_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBN6227948.1"
                     /translation="KYIIYNKKKKEDYLKRIAYTDSLTGADSIDKFKINSNKLFAKNN
                     PEEYALFYMDVDKFKYINDMFGYDMGNNTLIHISDTIASELKEDEIFARVSADHFVLL
                     IKYKTDDDIKTRLNSIYNKVQIFSNPKINYYKLILDCGIYKISKSDNDINTIMDRANT
                     ARKTIKGGHKNSFAFYDKEMHKKILKEKEIENSMVDALNNGEFVVYFQPKYRLSDYQI
                     IGAEALVRWDNPQKGLIPPIEFIPVFERNGFIVNIDFYVFEEVCKKIREWMDEGQEVV
                     PISVNLSRMHFVNSNFIEKFKLIVDKYKIPTSLIELELTETAVLDNIEGLLDTMNNLK
                     ENGFVISMDDFGTGYSSLNLLKELPVDILKLDRAFFTEKDESNNEKIVISNVIKMAKE
                     LKMKVISEGVETISQVEFLKQIGCDMVQGYLFSKPMPVKEFEKIAFKKE"
     gene            1648..1860
                     /locus_tag="L3M60_003318"
     CDS             1648..1860
                     /locus_tag="L3M60_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBN6227949.1"
                     /translation="MMPLILSNTGEEVVIKKISGSDKTRTFLNSIGFVVGTNVKIVSK
                     IDGNLIIDVKDTRVALDKKMASRIII"
     gene            1873..2094
                     /locus_tag="L3M60_003319"
     CDS             1873..2094
                     /locus_tag="L3M60_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HBN6227950.1"
                     /translation="MNRLKDIKCGETVKVKKLEGEGATRRRIMDMGITRGVNIFIRKV
                     APLGDPIEITVRDYELSIRKSDAEKIIVE"
     gene            2197..>3918
                     /gene="feoB"
                     /locus_tag="L3M60_003320"
     CDS             2197..>3918
                     /gene="feoB"
                     /locus_tag="L3M60_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HBN6227951.1"
                     /translation="MSIKIGLIGNPNCGKTTMFNGLTGSSQYVGNWPGVTVEKKGGKL
                     KGNKDVEIVDLPGIYSLSPYTLEEVVTRNFMLDDKPDAVINIVDASNIERNLYLTTQV
                     LELGIPTVIALNMMDIVNKNGDKINIKELSEVIGCPVVEVTAVKGQGIMEAAEKAVEL
                     ASSNNKLNFKLPFVDESKEAIEKIEKIIEEKTPYIDVETRWLAIKLFERDENVIQKLD
                     ISKTILNSIEEITRNCEDELDDDSESIITANRYEFISSIISNIIKKNRKGKETVSDKI
                     DKIVTNRILALPIFALIMWGVYYIAVSSLGTIATDWTNDVLFGEIIQGNVSNFLASLN
                     VAEWLQGLVVDGLIGGVGAVLGFVPQIMLLFLLLSILEDCGYMSRVAFIMDRIFRKFG
                     LSGKSFIPMLISSGCGVPGVMSTRTIENDRDRKMTIMLTTFIPCGAKVPIIALFAGAL
                     FGGASWVAPSMYFLGIAMIIICGIILKKTSLFAGEPSPFVMELPQYHIPSIKGVLIHM
                     WDRGKAFIVKAGTIIFVACGVIWFLQSFNWSLQMVDAGDSILASLGNIVAPIFAPLGF
                     GNWQSSVA"
BASE COUNT         1521 a          376 c          762 g         1259 t
ORIGIN      
        1 taaatatata atttataata aaaagaagaa agaggactat ctaaagagga tagcatatac
       61 agattcattg acaggagcag acagtataga taaatttaaa ataaattcga ataaactgtt
      121 tgctaagaat aatccagaag aatatgcgtt gttttatatg gatgttgaca aatttaaata
      181 tataaatgat atgtttggat atgatatggg aaataatact ttgatacata tatcagatac
      241 tattgcaagt gaattaaagg aagatgaaat ttttgcaaga gtttctgctg accattttgt
      301 tcttcttatt aagtataaga cagatgatga tattaaaaca aggttaaaca gtatttataa
      361 taaggttcaa atatttagta atcctaaaat aaactattat aaattaattt tagattgtgg
      421 tatatataaa ataagtaaaa gtgataatga tataaacact atcatggata gggctaatac
      481 tgcaagaaaa actataaaag gtggtcataa aaattcattt gctttttacg ataaggaaat
      541 gcataaaaaa atattaaaag agaaagaaat tgaaaactca atggtagatg ctcttaataa
      601 tggagaattt gttgtttatt ttcaacctaa atataggctt tcagattatc agattatagg
      661 agcagaggct cttgttagat gggataatcc acaaaaaggt ctaataccac ctatagaatt
      721 tattcctgta tttgagagaa atggttttat tgtaaatatt gatttctatg tatttgagga
      781 agtgtgtaaa aagattagag aatggatgga tgaaggtcaa gaagtagtac ctatatctgt
      841 aaacttgtcc cgaatgcatt ttgtaaatag taattttata gagaagttta agttaattgt
      901 agataaatac aaaattccga caagccttat agaattagaa ctaacagaaa cagcagtatt
      961 agataatata gaaggtttac ttgatacaat gaacaatttg aaagaaaatg gatttgtaat
     1021 ttctatggac gattttggta caggatattc ctctcttaat cttttaaaag agttgcctgt
     1081 tgatatatta aaactagata gagcattttt tacagaaaaa gatgagagta ataatgaaaa
     1141 aattgttata tctaatgtca ttaaaatggc aaaggaactg aaaatgaaag taatatcaga
     1201 gggagtagag actatttcgc aagtagaatt tctaaagcaa attggttgtg atatggtaca
     1261 aggatatttg ttttctaagc caatgcctgt aaaagagttt gaaaaaattg cctttaaaaa
     1321 agaataaagc aatgtttaag taatgacata tataataaag gtgctttaat atagttgagt
     1381 atactatttt agagcatctt tatttttgta ttaattttta ctgttttatt aatataatga
     1441 atcaaatgag aaaaaaaatc aaaagaatac aataatcact tgcaaaaaac atactattgt
     1501 ggtagactta aatcaaaatt aggagttgta aaaagaagga aaaaaataga ggattatttt
     1561 aatagattaa gatttaagat attgtaattt atatattaga tgatgattgc ttgtggattt
     1621 aaatcagttt aagaggaggg ttactatatg atgccattga tattatcaaa tacaggtgaa
     1681 gaagtagtta taaagaaaat ttcaggaagt gataagacga ggactttttt aaatagtata
     1741 ggttttgtag taggtacaaa tgttaagata gtttctaaga tagatggtaa cttaataata
     1801 gatgtaaaag atactagagt tgcattagat aaaaaaatgg caagtagaat aataatatag
     1861 ggaggtaata atatgaacag gctaaaggat attaagtgtg gagagactgt aaaagtcaaa
     1921 aagttagaag gcgaaggagc tacacgaaga cgtataatgg atatggggat tacaagaggt
     1981 gttaatatat ttataagaaa agtagctcct cttggtgacc caattgagat aacagtcaga
     2041 gattatgaac tatcaattcg taaatcagat gcagaaaaga ttattgtgga ataataaaat
     2101 taattagtta gaataggttt agtgcgatta tataatgaga atgattttct gtatcatgaa
     2161 tagttatgta cttaaataat aatggaggga atggaaatgt caataaaaat aggtctcata
     2221 ggaaatccta actgtggtaa aacaacaatg ttcaatggtc ttacaggttc atcacaatat
     2281 gttgggaact ggccaggagt aacagttgaa aaaaaaggtg gaaagttaaa aggaaataaa
     2341 gatgtggaga tagtagattt accaggtatt tattctttat ctccatatac attagaagaa
     2401 gtagttacac gtaattttat gttagatgat aagccggatg cagtaataaa tattgtagac
     2461 gcatcaaata tagagagaaa tttatatctt acaactcaag ttttagagct gggtataccg
     2521 accgttatag ctcttaatat gatggatatt gttaataaga atggtgataa gataaatatt
     2581 aaagaactat ctgaagttat aggttgccca gtggtagaag taacagcagt taaaggtcaa
     2641 ggaattatgg aggctgctga aaaggcagtt gaacttgcaa gtagtaacaa taaattaaat
     2701 ttcaaattac ctttcgttga tgaaagtaag gaagcaatag aaaaaattga aaaaataata
     2761 gaagaaaaga ctccatatat agatgtagaa acacgctggt tagctataaa gttatttgaa
     2821 agagatgaaa atgttataca aaaacttgat atttctaaaa caatattgaa tagcattgaa
     2881 gaaataacta gaaattgtga agatgaattg gatgatgata gtgaaagtat aataacagcc
     2941 aatcgttatg agtttattag ttcaataatt tcaaatataa taaagaagaa tagaaaaggt
     3001 aaggaaactg tatcagataa gatagataag attgtaacaa atcgtatact agcattacct
     3061 atttttgcac ttataatgtg gggagtttac tatatagcag taagttcatt gggtactata
     3121 gcaactgatt ggacaaatga tgtactgttt ggagaaatta ttcaaggtaa tgtaagtaat
     3181 tttctagctt ctttaaatgt agcagagtgg ttgcaaggac ttgtagttga tggtttaatt
     3241 ggtggagttg gagcagtatt aggatttgta ccacaaatca tgcttttatt cttattatta
     3301 tcaatattag aagattgtgg gtatatgtca cgtgtagctt ttataatgga tagaattttc
     3361 cgtaagtttg gtctttcagg aaaatcattt ataccaatgc ttataagttc aggttgtgga
     3421 gttccaggag ttatgtcaac tcgtactata gagaatgata gagatagaaa aatgactatt
     3481 atgttaacta cattcattcc atgtggagct aaggttccaa taatagcatt atttgcaggg
     3541 gcattatttg gaggtgcttc atgggtagct ccatcaatgt atttcttagg tattgcaatg
     3601 ataatcattt gtggaattat attaaagaaa actagtttat ttgcaggaga accatctcca
     3661 tttgttatgg aattacctca atatcatatt ccaagtataa aaggtgtttt aatacatatg
     3721 tgggatagag gaaaagcatt tatagtcaaa gcaggaacaa ttatatttgt tgcttgtgga
     3781 gtgatttggt tcttacaatc atttaattgg tcattacaaa tggtagatgc tggagatagt
     3841 atattagcta gcttaggaaa tatagttgca ccaatatttg cgcctcttgg atttggaaat
     3901 tggcaatcat cagtagca
//
LOCUS       DAFNNB010000210         3888 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0215, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000210 DAFNNB010000000
VERSION     DAFNNB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0215"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            63..857
                     /locus_tag="L3M60_003321"
     CDS             63..857
                     /locus_tag="L3M60_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6227952.1"
                     /translation="MIEVDKLCKSFTRVVKDDKNKSSIRKLKKIKTKKEEFFAVNNVS
                     FKVIEGEIVGILGPNGAGKTTLLRMLGGILTPTSGSINVSGYDYSIDRNSAKKEIGYL
                     SGNTKLYGRLSPRELLTTFASLYEMSKEEIEESIENVVKIMDMSEFIDNRIENLSTGQ
                     TQRTSIARCLIHSPKVYIFDEPTLGLDVLSSASIIDFMKNEKLRGKTVLYSTHYMEEA
                     ETLCDKIFMIHNGEIIASGTPQSLKEESGVNNLRDVFIKLARREDI"
     gene            858..2918
                     /locus_tag="L3M60_003322"
     CDS             858..2918
                     /locus_tag="L3M60_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBN6227953.1"
                     /translation="MRSKIVKYIFKKEMLDILRDKKTLFMMIVVPLLLYPLLMLLMIQ
                     VMNMSASSMNSKDINIAFNNKPNKVLVSMIEDDNLEKNIKNTSSDKGKIRVLDVDNYK
                     KSLEENKIDAYIKIEEKKSSTSYRIYINSSQENSSNAVKRIETVLDKYKRSTIEKTLS
                     EKGLDVQATLEPITYSTVDVAKTEEVAGYFLGQILPFILIIGVLLGSIYPAIDVMAGE
                     KERGTLETLFTLPISNLELVMGKYMAVSLSAIVTAILNILSMGLTMTFILVSGGISNQ
                     FGFGNFNYGDLVLPIITTLICICLFSMVVSAISMCVCSLAKSFKDAQNYITPLMLIIM
                     IPSYVSMIPNIVLDRVTAVIPVVNISLLIKSVLSFKSDLNLIALVLASNVAFVILAII
                     LLSKMFNSEEILFGNSKSFSFLEKRSNIKKGSIPTVSDGVILYAVGLVLLIYVGSLVQ
                     IKFGIVGIIMTQFIIISLPLLFAYYIKADFKEVFSLKIPKLKHVFGSIVLWIGGYILI
                     MIITQLILFLFPQSMEVAENLNKALFMKDSVLLNLLVIALLPAICEEIFFRGFIFSSF
                     SKSKDKNKSIKLTIICSSVLFGIMHMDFIRIIPTSILGIIFAYSVYKSGSIFVSMLLH
                     FLNNGVAVMLNHYTTGNIINLYKFVEVDFSNLNVLEFALILLISLILVMLGARALGRK
                     SLRN"
     gene            3525..>3888
                     /locus_tag="L3M60_003323"
     CDS             3525..>3888
                     /locus_tag="L3M60_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBN6227954.1"
                     /translation="MRNFKKKLMFFLFICIILQITCTTSVYSLCKIYKNNSVNNFEFI
                     VSNNQLISKYALQMILIMATICIILIFYIIYDKLNFKIKLQKIAYTDNLTGANTIDKF
                     VIDANKILCKNTQVKYALL"
BASE COUNT         1488 a          384 c          601 g         1415 t
ORIGIN      
        1 aaggtttata tgttatattt atataaatat aacatatgaa tgagaaagga gtagtttgat
       61 aaatgattga ggtagataaa ttatgcaagt cttttacaag ggttgtaaaa gatgataaga
      121 ataaaagttc aataagaaag ttaaagaaaa taaaaactaa aaaagaagaa ttttttgctg
      181 taaataatgt ttcatttaaa gttatagaag gtgaaattgt tggaatatta gggccaaatg
      241 gtgcaggaaa aaccacatta cttagaatgt taggtggcat attaacacca acatcaggaa
      301 gtataaatgt ttctggatat gattattcaa tagatagaaa ttcagccaaa aaagaaatag
      361 gctatctgtc tggaaataca aaactatatg gaaggctttc tcctagagaa cttctaacaa
      421 cttttgcaag tctatatgag atgtcaaaag aagagataga agaatcgatt gaaaatgttg
      481 taaaaataat ggatatgagt gaatttatag ataatagaat tgaaaatcta tcaactggtc
      541 agacccaaag aacatctata gctcgttgtt taatacattc tccaaaagta tacatttttg
      601 atgagccaac actaggtctg gatgttttaa gtagtgcatc cattatagat tttatgaaga
      661 atgaaaaact aaggggaaaa actgttcttt actcaactca ttatatggaa gaagcagaaa
      721 ctttatgtga caagattttt atgattcaca atggggaaat aattgcatca ggaactccac
      781 agagtttaaa agaggaatct ggagtaaata atttgagaga tgtatttata aaacttgcca
      841 gaagggagga tatttaaatg agaagtaaga ttgtaaaata catatttaaa aaagaaatgt
      901 tagacatatt gagagataaa aagactttat ttatgatgat tgttgtgcca cttcttttat
      961 atcctttact tatgttgttg atgattcaag taatgaatat gtctgcaagt agtatgaata
     1021 gcaaggacat taatatagct ttcaataata aaccaaataa agtgttagtt tccatgattg
     1081 aagatgataa tttagaaaaa aatattaaaa atacttcaag tgataaagga aaaataagag
     1141 tgttagatgt agataactat aaaaagtcat tagaagaaaa taaaattgat gcttatataa
     1201 agatagaaga aaaaaaatca tctacaagtt atcgaatata tataaattct tctcaagaaa
     1261 attcttctaa tgcagtaaaa agaattgaaa ctgttttgga taaatataaa agatctacta
     1321 tagaaaagac tttatctgaa aaaggattag atgtacaagc tactttagaa ccaataacat
     1381 atagcactgt agatgttgca aagacagaag aagtggcagg atacttttta ggtcagatac
     1441 taccatttat attaataata ggagttttac taggttcaat atatccagca atagatgtta
     1501 tggctggaga aaaggaaaga ggtacgttag aaacattatt tacacttcca atatcaaact
     1561 tagaactagt tatgggaaaa tatatggcag tttctcttag tgcaatagta acagcaattt
     1621 taaacatttt atctatggga ctgactatga catttatttt agttagtgga ggtattagta
     1681 accaatttgg ctttggaaac tttaattatg gagatttagt cttgccaatt ataactacct
     1741 taatttgcat atgtcttttt tcaatggtag tttcagcaat aagtatgtgt gtatgttcat
     1801 tagcaaaaag ttttaaagat gcccaaaact acataacacc tcttatgtta attattatga
     1861 taccatcata tgtttctatg atacccaata tagtattgga tagagtaaca gctgtaatac
     1921 ctgtagtaaa tatatcttta cttataaaaa gtgtattatc ctttaaaagc gatttaaatt
     1981 taatagcttt agttttagca tcaaatgtag cctttgttat attggcaata atactactgt
     2041 ctaagatgtt caattctgag gaaatacttt ttggtaatag taagagtttc tctttcttag
     2101 agaaaagaag taatataaaa aaagggtcta taccaactgt aagtgatggt gttattttat
     2161 atgcagtagg attagtttta ttgatttatg ttggttcttt agttcaaatt aaatttggta
     2221 tagtaggtat tataatgaca caattcatta taatttcttt gccattatta tttgcatact
     2281 atattaaagc tgattttaaa gaggtattta gcttgaaaat tcctaaatta aagcacgtgt
     2341 ttgggagcat tgttctttgg attggtggtt atatacttat tatgattata acacagctaa
     2401 ttttattctt attccctcaa agtatggaag ttgctgaaaa tttaaataag gctctcttta
     2461 tgaaagatag tgttttattg aatttactag taatagcatt attaccagct atatgtgaag
     2521 aaatattttt taggggattt atattttctt catttagtaa atcaaaagat aaaaataagt
     2581 ctatcaagct tactataata tgtagtagtg tactgtttgg tattatgcat atggatttta
     2641 ttagaataat tccaacatca atattgggta ttatatttgc atacagtgtg tataaatctg
     2701 gttcaatatt tgtatcaatg ttattgcatt ttttaaataa tggtgtagca gttatgttaa
     2761 atcattatac aactggaaat ataatcaact tatataaatt tgttgaagtt gatttttcga
     2821 atttaaatgt acttgaattt gcgttaattc ttctaataag tcttatatta gttatgttag
     2881 gagcaagagc tttaggtaga aagtctttaa gaaattaagt ataaatttaa tatacattaa
     2941 aatattaata agcactaaag tatatggatg tatcctgtat ataaaaatac tttagtgcta
     3001 ttttaattat atgaattatt tattttagaa atttatattt aaataatcaa gtataaagtg
     3061 agatattaaa aaatcaactt agtaattatt tgatttattc acttaaaaaa attaattatt
     3121 gatttgtcga aactcttgca aaaataaaaa gataaagtat gaatatgttg caaacactgt
     3181 gttttaatga aaagtagtca tttcttgtaa aatggatagt ttatatttac cttaattttg
     3241 taaaattttc tgttactata attatagaat gtagtatatt cattgtctta actattgatt
     3301 tatatatttc atataggtat aatagaaaat aaattggatt attttggcat ataattgttt
     3361 attaaaatgt gcttattttt atttaaattg tctgaaaaat tttattttta tattataatt
     3421 ttaatatttt cttttcaaaa ctatccattt tacaagaaat ggaaactttt gagatgtact
     3481 tgtttgccaa taaaatagag gtgttttaat aggaggaaat ttttatgagg aattttaaaa
     3541 aaaaattgat gtttttttta tttatttgta taattttaca aattacatgc acaacatcag
     3601 tctattcttt atgcaaaatc tataaaaata actccgtaaa taactttgaa tttattgtgt
     3661 ctaataatca gcttattagc aaatatgcat tacaaatgat acttattatg gctacaatat
     3721 gtattatact tatattctat ataatatatg ataaattaaa ttttaaaatt aaattgcaaa
     3781 agatagcata tacagataat cttacaggag caaatactat agataaattt gtaattgatg
     3841 ctaacaaaat actatgtaaa aatactcaag taaagtatgc tttattat
//
LOCUS       DAFNNB010000211         3879 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0216, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000211 DAFNNB010000000
VERSION     DAFNNB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0216"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(162..713)
                     /locus_tag="L3M60_003324"
     CDS             complement(162..713)
                     /locus_tag="L3M60_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan transporter"
                     /protein_id="HBN6227955.1"
                     /translation="MKTNTKKLTLNAILLAMGLLIHQITPAIGLPMKPDVPLAMLFVI
                     LVLNREDYKTCLIAGIVTGIFTALTSSFPGGQIPNVIDKTLTANIVFLLMHISYKMPF
                     IKKLAKKTQDTVVVAIIMPIGTLVSGTIFLLAAQAIVGLPGASFTALFLAVVLPAVLI
                     NLVAGIFLFKIVSLSIRRVSYQA"
     misc_binding    complement(865..1128)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1557..1964)
                     /locus_tag="L3M60_003325"
     CDS             complement(1557..1964)
                     /locus_tag="L3M60_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227956.1"
                     /translation="MNKKKNLIGMAISFLLIISIINPLQVEAETIIESSVYISSIQEN
                     CTYNNTKFKTIKSRSRHKISKSNKSKVKLKKRSIRTKKYGYQTINKYKIRNARRHNIL
                     TKLILLIIVIFIVIILVILIFIKRKKKSFTKNN"
     gene            complement(2302..2787)
                     /locus_tag="L3M60_003326"
     CDS             complement(2302..2787)
                     /locus_tag="L3M60_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBN6227957.1"
                     /translation="MISELMLKNFGESSINFKTKIKLNLREKPDLNSLKLKSIPGGKI
                     VKLKCVDGIWAEVESNYDKGWLLYKYLERLSNAKNDSNDTIKNRKKNYIGNIRTNGLS
                     LELRNDRTLESKIITTIPDGFKVEVCYNVGKWARVNIDKNEKRYSGYVYNQYIEEYLG
                     L"
     regulatory      complement(2870..2963)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            complement(3313..3651)
                     /locus_tag="L3M60_003327"
     CDS             complement(3313..3651)
                     /locus_tag="L3M60_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227958.1"
                     /translation="MKKYKIGGNFYMEVTLHTGEINFFKEHEVAKNYIGELYNFIEEY
                     KLSMETVSILTNIDIKKLNDFYIGKGTLHYDELVLINSVIIMPFIDVFKTAKHNYEMI
                     IKSSKKYEED"
     gene            complement(3638..3865)
                     /locus_tag="L3M60_003328"
     CDS             complement(3638..3865)
                     /locus_tag="L3M60_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6227959.1"
                     /translation="MAMVSISFGEKIKIILKRKNMTIGELAEKTGQTRQNLSNKFSRD
                     NFSEKEIRKFAKILECEFDFYFIIKDTNEKV"
BASE COUNT         1320 a          568 c          317 g         1674 t
ORIGIN      
        1 tgttagatta agtgtaattg gtactatata aatattttat taaaaataaa tatataatac
       61 caaatctttg aacaacataa gaataaaata tttataatct actttattct tacgttgttc
      121 agttattata atagtttata aagtaaaata ttatttcgta tttaagcttg ataactaact
      181 cttcttattg atagactaac aatcttgaat aaaaatattc ctgcaactag atttattaaa
      241 acagctggta atactactgc taagaataaa gctgtgaatg aagcccctgg aagaccaact
      301 atagcttgag ctgctaataa aaagatagtt ccacttacaa gagttcctat tggcattatt
      361 attgcaacta ctacagtatc ttgtgttttc ttagctaatt tttttatgaa aggcatctta
      421 taactaatgt gcattaataa aaatactata tttgctgtaa gagttttatc tattacatta
      481 ggaatttgac ctcccggaaa acttgatgta agtgctgtaa atattcctgt tactatacct
      541 gctattaaac aagttttata atcctctcta tttaaaacca atattacaaa taacattgct
      601 agtggtacat ctggtttcat tggtaatcct atagctggtg tgatttgatg tatcaataat
      661 cccatcgcta aaagtattgc atttaaagtt aatttttttg tatttgtttt cattttttgt
      721 acttcctcct atttttttaa ttttcaattt atttttttgc caacctacta tatcatcatc
      781 tttagctttg gaaattcata acatcttttt caccaccttc tatcttttaa attttcagta
      841 aaataaaaaa actccgtcct aaactaatag gacggagatt atccgcgttg ccacctaaat
      901 tatataaaaa ttaatataaa attcaatttt catatatctc attatcaggt acttacatac
      961 cttattcgct ataacgtgcg actgacgata aagtctactc tcaaaactat gatttcaatt
     1021 tattcctcta agggccattc atcaaatttt ttctcgctaa gattccacca tcctcagctc
     1081 tctatactga acctaaattt aactactctt ccttgtcata ggatttaatt tattgtatta
     1141 aattttgttg ttaaaataat gatattcttt tttctaaaaa atgtcaacac tttttataaa
     1201 aaatatattt tgtaatctat ttttttataa aaaaagtaaa ttacactata catttctttc
     1261 cacatacaaa attctatgaa taattacttt aatagtctaa taatctgatt tttagctcta
     1321 attctgcctt tattaatttt atgaataatt attgatgtaa ttttatattt tgatgcatga
     1381 ggaactaaat tcttcaaagt taattaagta catttatcaa aaattcatct catttaccat
     1441 catttaatac tcatttctgt attatttata tacctacttt gaaacagaat actactctgt
     1501 tttaattaca taatgttatt aacaaaaata ataataatta gaaaaatgat attcttttaa
     1561 ttatttttag taaaactttt tttctttctt ttaataaata ttagtatcac taaaattata
     1621 acaataaata taactattat taacaatatt aattttgtta gaatattatg tcttcttgca
     1681 tttcgtattt tatatttatt tatagtttga tatccatatt ttttagttct tatacttcgt
     1741 tttttcaact ttactttact tttattagac ttactaattt tatgtctact tctagattta
     1801 atagttttaa atttagtatt attatatgta caattttctt gtattgaaga aatgtagaca
     1861 ctagactcaa taatagtttc cgcttctact tgtaatggat ttatgatgct gattataagt
     1921 aaaaaagata tagccatccc aatcaagttt ttcttcttat tcatattttc ccccttataa
     1981 aaatttttat ctacttcatt ataatatata catatataac taaaaatcat aaattttttc
     2041 aaatttctta tatattttat tatctttaat gttattttta ttatcataac taaaattttt
     2101 caaagtagta ttaatttatg aaactttata tataaagtat aaattaaatc tattttcaat
     2161 taaatttata attactttac agtaaaaata aattattttt ttctctttag ttctataata
     2221 atgtttcgac aaaaaaagaa cccctttaaa gggattcttt tttgtaattt attaatatat
     2281 ttaaagctca ctagtatcat attataaacc taaatattct tctatgtatt gattatatac
     2341 atatccagaa tatcgttttt catttttatc aatattaact cttgcccatt tcccaacgtt
     2401 ataacatact tcaactttaa aaccatcagg aattgtagtt ataattttag attctaatgt
     2461 tctatcattt ctaagttcta aacttaatcc atttgttctt atatttccta tataattctt
     2521 ttttctattt tttattgtat cattactatc atttttagca ttacttaatc tttctaaata
     2581 tttataaagt aaccagcctt tgtcataatt actctcaact tctgcccata ttccatctac
     2641 acattttaac ttaacaattt taccccctgg tatacttttt aattttaaac tatttaggtc
     2701 tggtttttct cttaaattca atttaatttt agtcttaaaa ttgatgctag attctccaaa
     2761 attttttaac attaattcag atatcatctt aagccctcct cttgccaaat attaagttat
     2821 taaaatatat aactctaaaa atatttttaa caaaaagaat cattatcttt ttggcaactg
     2881 gctgacatga cctttattat ataaagattt tagtccctat agttttgcgt cattagattt
     2941 atctaatatt gcctatatat gaatgataac ataatttaaa caattaaaaa ataatattac
     3001 ctcaaatttt atattaatta aatataaatt aaatttttaa ttaataataa tcaaaaaata
     3061 tctttatatt tattcaatta ctattattta tggtcaatat aaaatacagt aatttaccta
     3121 tagtgttact tatattatat atatatggct atttttcaac attttataat atttacagca
     3181 tttatttttt taacttttgt cagtaaaata ttttatccac aatttttata tgagttttcc
     3241 ataacttatc accagcttta tcaacaacaa taaaaggtag ctactactta tatagaatct
     3301 accttttttc tattaatctt cttcatattt tttactgctt ttaattatca tttcatagtt
     3361 atgtttagca gttttaaata catctataaa aggcattata ataacgctgt ttataagtac
     3421 taattcatca tagtgtagag ttccctttcc tatataaaag tcatttaact tctttatatc
     3481 tatattagtc aaaatagata ctgtttccat tgataactta tattcctcta taaaattgta
     3541 taattctcct atataattct ttgcaacctc atgttcttta aaaaaattaa tctctcctgt
     3601 atgcaaagtt acttccatat aaaaatttcc tcctatttta tactttttca tttgtatctt
     3661 taataataaa ataaaaatca aattcacact caagtatttt tgcaaatttt ctaatttctt
     3721 tttctgaaaa attatctctt gaaaatttat ttgaaagatt ttgtcttgtt tgccctgttt
     3781 tttctgctag ctcacctata gtcatatttt ttctttttaa tattattttt atcttttctc
     3841 caaatgaaat tgacaccata gccacctcgc ttttctata
//
LOCUS       DAFNNB010000212         3802 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0217, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000212 DAFNNB010000000
VERSION     DAFNNB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0217"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(66..884)
                     /locus_tag="L3M60_003329"
     CDS             complement(66..884)
                     /locus_tag="L3M60_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003432306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6227960.1"
                     /translation="MFKIGDFSKLSKISIRMLRHYDEIGLLTPSHTNKTNGYRYYSAD
                     QLSTTNRIHALKDMGFGLYSIKEILTEYNDKESLIKYLNIHHSQVKEQLEDTQKKLLK
                     IESTIKRIGGNDIMKNYDVTIKNFASKYMMTLRMVIPTYQDEGMLWHQAFLETKDQNV
                     QIEPPRHSKAVFYDTGYKEDYVDVEVQVAVSGKYKDTEHVKFKTVPSVTAATAIVNGN
                     FNQVADACEAIGTWISDNDYDVDGPMFNIYHVSPGNDNNPDNWVTEVCFPVKKK"
     gene            1052..1633
                     /locus_tag="L3M60_003330"
     CDS             1052..1633
                     /locus_tag="L3M60_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6227961.1"
                     /translation="MKSNDKDARERIIEVTLNLLNEVDDIEEITVRKIAERANVGVGL
                     INYHFKTKDNLLSTAIGDVMSNIIAELYDDSVYTLRPIEDLKNLLKKLCDTGLHYEKV
                     LPFVLNQCIANGDMQAELDIVPMLRKIFGNKKDEMSLRIIALQIILPIQISALSTESF
                     QLYSGINIKNKYERDKFIDILIENIIGEGVDVR"
     gene            1630..1824
                     /locus_tag="L3M60_003331"
     CDS             1630..1824
                     /locus_tag="L3M60_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227962.1"
                     /translation="MKIITIGSSLITVLLFLSTMVCGFWIKNNKVTDASSIKFHMNSA
                     IFTGIFLLISTILLIIYIKK"
     gene            complement(1988..3730)
                     /locus_tag="L3M60_003332"
     CDS             complement(1988..3730)
                     /locus_tag="L3M60_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M3"
                     /protein_id="HBN6227963.1"
                     /translation="MVKSKKKIFVSLILIAVFIIFASYIRNYTNDTKKDSVRGTIPFE
                     KMEYKRPNIQSICENINKCNDKLLTAKTAKEQLNLFNEVDKIYQDFYSTLTIAKIRNN
                     IDSSEEFYSNEYKYLMSKSVDVDMSYKDFQDRFVTSKFSKELEKELGKDTFNYLKNIG
                     KLNSKEVEGLLKKEQDLVVKYEDLLSKSTVSIDGIEIDFEEALSRPNLSPEEYVKIYS
                     DYLKKYNPIFGNIFLELIQTRTEIASKQGFKNYIDYAYMNLNKDYSQEEAKKFRQDVK
                     DYIVPLYREISSKPSDSSIYIKVYKNRSFRKFDKVLEDISPKLKESFDYMKKYDLYDY
                     SSGKNKSPGGYTTYINKYKAPFLFNTWDNSFLGVTSFAHEFGHFYNYYNSINLNNKMQ
                     PSIDICEVHSTSLEILFYKYFDDFFGKQSEAIKKEHLSIVLNTIIDACLYDEFQEIIY
                     KNPYMSLDDINKLFFDLEEEYGVSNNILKEKNAPFWILVSHNFQVPFYYLSYGLASDV
                     SLQIWQLSQGDYRKAVDVYMDFLNQNTDAGFKDVVEKVNLQLPFQDGNLEEISSTLYD
                     YFGIDNPLELKNAS"
BASE COUNT         1335 a          512 c          503 g         1452 t
ORIGIN      
        1 acataacact aaaagtaatt tgtctaacaa attaaaatat tatccaatta atatataaga
       61 taaaattact tctttttaac tgggaagcat acctctgtta cccaattatc aggattatta
      121 tcattaccag gacttacatg ataaatatta aacataggac catccacatc gtagtcatta
      181 tctgatatcc aagtaccaat agcttcacaa gcatctgcaa cttgattgaa atttccatta
      241 acaattgcag ttgcagcagt cacacttgga acagtcttaa atttaacatg ctcagtatct
      301 ttatactttc cagatacagc aacttgtact tcaacatcta cataatcttc tttatatcca
      361 gtatcataaa agactgcctt agaatgtctt ggtggttcaa tttgaacatt ctggtctttt
      421 gtctctaaaa atgcttgatg ccataacatt ccttcgtctt gataagttgg tataaccatt
      481 ctaagtgtca tcatatattt tgatgcaaaa ttctttatag ttacatcata atttttcata
      541 atatcatttc ctccaattct tttaatagta ctttctattt ttaatagttt tttctgtgta
      601 tcttctaatt gttcttttac ttgagaatgg tggatattta gatattttat aagactttct
      661 ttatcgttat attctgtcaa aatttctttt atagaataca agccaaagcc catatctttt
      721 agtgcatgga ttctgtttgt tgtagaaagt tggtctgcgc tatagtatct atatccatta
      781 gttttattcg tatgagaagg agttaaaagc cctatttcgt cataatgacg taacatacga
      841 atactaattt tacttagttt tgaaaaatcg cctatcttaa acatgttttt gtctccttta
      901 taaaggtact tttagcttaa tattaatata aaccatatca taatgttaga gtcaactaaa
      961 attataatta aattatattg acatatgatg aaaaaaatat tatactgaac atgttcagaa
     1021 ctaaaaatgt taattgtttt ggaggtccat tatgaaaagt aatgataaag atgctagaga
     1081 acgtataatt gaagttacat tgaatctttt aaatgaagta gatgatattg aagagattac
     1141 agttagaaaa attgctgagc gtgcaaatgt tggagttggt ttaataaatt atcattttaa
     1201 aacaaaggat aatttgttga gtactgccat aggagatgtg atgtcaaata taatagcaga
     1261 gctatatgac gatagtgtat atactctaag acctattgaa gatttgaaaa atttattaaa
     1321 aaaattgtgt gatactggat tacattatga aaaagtgctt ccttttgtgt tgaatcaatg
     1381 tatagccaat ggagatatgc aagcagaact tgatattgtc cctatgcttc gtaaaatttt
     1441 tggaaataaa aaagatgaaa tgagtctaag aattattgcc ctgcaaatta ttttaccaat
     1501 acagatttct gctttatcta cagaatcatt tcagttatac agtggtataa atataaagaa
     1561 taaatatgag cgagataaat ttattgatat tttaatagaa aatattattg gagagggtgt
     1621 tgatgtaaga tgaaaattat aactattggt tcaagtctta tcactgtttt actttttctt
     1681 tcaactatgg tttgtgggtt ctggattaag aataataagg taacagatgc aagttctatt
     1741 aagttccata tgaatagtgc tatttttaca gggatatttt tattgatttc cacaatcctt
     1801 ttaattatat atataaaaaa ataataatat tattaataga taaagtatca gaaattataa
     1861 agacaagagt cttaaaaatt tctgatactt taccatataa ttttattaaa cattaattgt
     1921 gtgtgaaaaa atatcactta tttcaatcta atgctaaatc ttaagagtaa gcataaagta
     1981 ttaaaaatta agaagcattt tttagttcaa gtggattatc tataccaaaa taatcatata
     2041 aagtagagct tatttcctct aaatttccat cttggaaagg taattgtaaa ttcacctttt
     2101 ctaccacatc cttaaatcca gcatcagtat tttgatttaa aaaatccata tatacatcaa
     2161 cagcttttct gtaatcacct tgtgataatt gccatatctg aagtgataca tcagatgcaa
     2221 gaccataact caagtaataa aaaggaactt ggaagttatg agatacaagt atccaaaaag
     2281 gagcattttt ttctttaagt atattattgg aaactccata ttcttcttcc aaatcaaaaa
     2341 acaatttatt gatatcatct agactcatat atggattctt atatataatt tcttgaaatt
     2401 catcataaag acaagcatca ataatagtgt ttaaaacaat cgataagtgt tcttttttta
     2461 tagcttcaga ttgtttccca aaaaaatcat caaaatattt gtaaaataga atttctaaag
     2521 atgttgaatg aacttcacat atatctatac ttggttgcat cttattattt aagttaatac
     2581 tgttgtagta attataaaaa tgaccaaatt catgggcaaa actagtaaca cctaaaaaag
     2641 aattatccca tgtattaaat aagaaaggag ctttatactt atttatataa gtggtatatc
     2701 ctccaggtga tttgttcttt ccagaagaat aatcgtacaa gtcatatttt ttcatatagt
     2761 caaagctctc ttttagtttt ggagatatat cttcaagcac cttatcaaac tttctaaatg
     2821 agcgattttt atatacttta atataaatag aagaatcaga aggtttagat gaaatctctc
     2881 tatataatgg aactatgtaa tcttttacat cttgtctaaa ttttttagct tcctcttgag
     2941 aataatcttt gtttaggttc atataggcat aatctatata atttttaaaa ccttgcttac
     3001 ttgctatttc agttcttgtc tgtataagtt ctaagaatat gttaccaaat atagggttgt
     3061 atttttttaa gtaatctgaa taaattttaa catattcttc agggctaaga tttggtcttg
     3121 acaaggcttc ttcaaagtct atttctatac catctattga tactgtagat ttagataaca
     3181 agtcttcata ttttacaact aggtcttgtt ctttcttaag taaaccttca acttctttag
     3241 aattaagttt tccaatgttc tttagataat taaatgtatc ttttccaagt tctttttcta
     3301 attctttaga gaatttagaa gttacaaatc tatcttgaaa gtctttgtat gacatgtcaa
     3361 catctactga tttactcata agatacttgt attcattaga gtaaaattct tctgatgaat
     3421 ctatgttatt tctaattttt gctatagtaa gtgtactgta aaaatcttga taaattttat
     3481 caacttcatt aaataaattt aattgttctt tagctgtttt agctgttaaa agtttgtcgt
     3541 tacatttgtt aatgttttcg caaattgact gtatattagg acgcttatat tccatttttt
     3601 caaaaggaat agttcctcta acagaatctt tttttgtatc attagtataa tttcttatgt
     3661 atgatgcaaa tataataaaa actgcaatta aaattagaga aacaaatatt ttcttcttgg
     3721 atttaaccat aaaaatcctc caatcatgga tatagttatt aattatttta ctatattaaa
     3781 tataaaaaat gcatgttaag aa
//
LOCUS       DAFNNB010000213         3730 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0218, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000213 DAFNNB010000000
VERSION     DAFNNB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0218"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            120..1058
                     /locus_tag="L3M60_003333"
     CDS             120..1058
                     /locus_tag="L3M60_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HBN6227964.1"
                     /translation="MNKRMKLIPYEINENPRGAKNKFPYGIKQMNVRGMWDEGYTGKN
                     IVVGIIDTGCDVSHPLLKGKIIGGANFSDDSNGNKNIYEDFNGHGTHVAGIIAASNYN
                     NEVMGVAPDCKLLIAKALNKDGTGTYQSIINAINFAVNNKVDIISMSLGGNKDDKNLK
                     NAVIQAVKNNISVVCAAGNNGDGDSSTSEYSYPASYAEVIEVGAINENYLVEKFSNSN
                     TTIDLVAPGRNIISTYMGNKLAIMSGTSMSAPYVSGSLALIKEWARDEFERDLDEAEL
                     YAQLIKCTRALGIPRTEQGNGYLYLNLYKYKNTGKR"
     gene            1572..2000
                     /locus_tag="L3M60_003334"
     CDS             1572..2000
                     /locus_tag="L3M60_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018112846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBN6227965.1"
                     /translation="MSKIYIVYWSGTGNTEKMANFIAEGVKSKGKTPEVLDVSLLKPS
                     DLKEENKFALGCPSMGAEQLEEGYMEPFVSELESMVSGKQIGLFGSYGWGNCEWMREW
                     EERMQNAGAVIIGGEGITAMEEPDEEAKDECIELGKMLAE"
     gene            complement(2153..2947)
                     /locus_tag="L3M60_003335"
     CDS             complement(2153..2947)
                     /locus_tag="L3M60_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase"
                     /protein_id="HBN6227966.1"
                     /translation="MEKYANMIKEKLNSGKVLCGVAVSLSDSVVSELIGISGYDFVWI
                     EGEHGALDRKDIQQHMIAAHAGGAASLVRLVNSDPDIVKPIMDMGTDIICFPFINTVQ
                     DAQKAVAACTYPPNGIRGYNPQRASYYGNMNYFDYVKNANNETLKMMLIEQKAGYDNL
                     EEIVKVKGVDIIALGPGDLSLDMGFSGYMDRIEIKDMISNATDICKKYKMPFVTFPSI
                     EKNSIETWVNIGSNMLCFAQDTTFITYVINQLLSFYNEIVPIKKQH"
     gene            3096..3464
                     /locus_tag="L3M60_003336"
     CDS             3096..3464
                     /locus_tag="L3M60_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6227967.1"
                     /translation="MKYSMTDVSKITEISSSAIRFYEKKGLLLPINRKPSGVRYFYEE
                     DINRLMFIKELKNAGLSIKDIHECFHFCDMGDDTIDKRIQLFQQHKQNIKNKIYELEN
                     CMDRIDEKIYYLKVKKMCKK"
BASE COUNT         1466 a          411 c          620 g         1233 t
ORIGIN      
        1 aataaaaaat atatcataaa tataaataag tagatatagg ttttaatttg atttataaac
       61 ctattataaa ataaaattta tgatttttat aagcaataaa atttttagga ggatatgtga
      121 tgaataaaag aatgaaacta attccatatg aaataaatga gaatccaaga ggtgcaaaaa
      181 ataagtttcc ctatggaata aagcaaatga atgttagggg aatgtgggat gaaggttata
      241 ctggtaaaaa tattgtagtt ggtataatag atacaggctg tgatgtatcc catcctcttt
      301 taaaaggaaa aataattggt ggggcaaatt ttagtgatga cagtaatgga aataaaaata
      361 tatatgaaga ttttaatggg catggaactc atgttgcagg cattatagct gcatctaatt
      421 ataataatga agttatggga gtagctccag attgtaaatt attaatagca aaagcattaa
      481 ataaagatgg tactggaaca tatcaaagta taattaatgc tattaacttt gctgtaaata
      541 acaaggttga tattatatct atgtctcttg gaggaaacaa agatgataag aatttaaaaa
      601 atgctgtcat acaagcagta aaaaataata tttctgtagt gtgtgcagca gggaataatg
      661 gagatggaga ctctagtaca agtgaatata gctatccagc aagttatgct gaggtgatag
      721 aagtaggtgc aataaatgag aactatttag ttgaaaagtt tagtaattca aatactacaa
      781 tagatttggt ggctccagga agaaatatta tatcaactta tatgggtaat aaacttgcta
      841 ttatgagtgg tactagtatg agtgcaccat acgtatcagg ctcattagca ctaattaaag
      901 aatgggcaag agatgagttt gaaagagatt tagatgaagc tgaattgtat gcacaattaa
      961 taaaatgtac gagggcactt ggaataccta gaacagaaca aggaaatgga tacttatatt
     1021 taaatcttta taaatacaag aatactggta aaagataatt tataatttta taataaaata
     1081 aatagggtat atagttaacg caaatgataa aaattaacta agtgtatatt ttgaaataga
     1141 tatatgaaaa tatttaataa taaggtctat agacctgtta tgcaattaga ataataaaaa
     1201 atatacagta ttatagatag cacatctgaa gaaagatgtg ttcttctata tgtctgtata
     1261 gatgctgtat ataaaaggta aattctagat tgaagttaag aaggtataag attatttata
     1321 atacgaacaa gaatattaag cgtgagaaat attcttataa aagatttaga aatataatgt
     1381 gatattatag gtaaataaag agtatagaaa ataaatgata tatattatca ataggtattg
     1441 aatttcaaaa tcatttgtga taatatatat ttagaaaaat aagagatttt agataaagga
     1501 attttttatt tttagaattt agttagcgat tgctaacttt cgattaatta aaaatactac
     1561 aggaggtaag aatgagtaaa atatatattg tttattggag tggaacagga aatactgaaa
     1621 aaatggcaaa ttttatagct gaaggtgtga agtcaaaagg caaaacacca gaggttttag
     1681 atgtaagttt gttaaagcca agtgatttaa aagaagaaaa taaatttgca ttgggttgcc
     1741 catctatggg agcagagcaa ctagaagaag gatatatgga accatttgtg tcagaattag
     1801 aatctatggt atcaggtaaa caaattggat tatttggttc atatggttgg ggaaattgcg
     1861 aatggatgag agaatgggaa gaacgtatgc aaaatgctgg tgcggtaatt ataggtggtg
     1921 aaggaattac agcaatggaa gaaccagatg aagaagcgaa agatgagtgt atagaattag
     1981 gcaaaatgtt agctgaataa atttgtatac tataaaaata gtataagtag taactaatga
     2041 taatagcagt gtataatact aagagagact aatattatga tacataagaa aacaccctta
     2101 atagagatag ataaaatatt agtctctttt agtataaaat tagatttata atttaatgtt
     2161 gttttttaat tggtacaatc tcattataaa aagacaataa ttgattgata acataagtaa
     2221 taaaggtagt atcttgtgca aaacaaagca tatttgatcc aatattaacc catgtttcaa
     2281 tactgttctt ttctattgaa ggaaaagtaa caaatggcat tttatatttt ttgcatatat
     2341 cagtggcatt agaaatcata tcttttattt caattctatc catgtatcca gaaaatccca
     2401 tgtccaagga taagtcacca ggacctaatg ctataatatc aacacccttg acttttacta
     2461 tttcttcaag gttatcataa cctgccttct gttcaattaa catcattttt aatgtttcat
     2521 tgtttgcgtt tttgacatag tcaaaataat tcatattccc ataataagag gctctttgag
     2581 gattgtatcc tcttataccg tttggaggat aagtacaagc tgctactgct ttttgtgcat
     2641 cttgtactgt attaataaat gggaaacaaa taatatctgt tcccatatcc attataggct
     2701 taacaatgtc agggtcactg tttactaatc gtaccaaaga agcagcacca ccagcatgtg
     2761 cagctatcat atgttgctga atgtctttac gatctaaagc accatgttct ccttcaatcc
     2821 agacaaaatc atatccagat ataccgatta actcacttac aactgaatca gacagtgaaa
     2881 cagctactcc acataagacc tttcctgaat ttagtttttc tttaatcata tttgcatatt
     2941 tttccatata catcatcctt tcttttgtca gtttactttt acacttcaaa gaatacaact
     3001 tcaatctaac ttgaagtcaa tacttttttg ttgactacag tataaggtat ttttataata
     3061 ttcttattcc tataaaattt atggaggtaa agattatgaa atattctatg actgatgtat
     3121 ctaaaataac agaaatttct tcatctgcta ttcgatttta tgaaaaaaaa ggtttattac
     3181 ttcctatcaa tagaaaacca agtggtgttc gctatttcta tgaagaagac ataaatcgtc
     3241 ttatgtttat caaagaatta aaaaatgctg gtttatctat taaagatatc catgaatgct
     3301 ttcatttttg tgatatggga gatgacacaa tagataaaag aatccaatta tttcaacaac
     3361 ataaacaaaa cattaaaaat aaaatatatg aactagaaaa ttgcatggat agaatagatg
     3421 agaaaatata ttacttaaag gtaaaaaaga tgtgtaaaaa ataatcctta tagaataagg
     3481 aaataatttt ttgatttttc taatataagt agtaaattca aatggcaaca tttgagaaaa
     3541 gattttttac aatatatttc tacgaagaat tggacaatgt tctatataac tatttatcta
     3601 tacaatgaaa agaaaattat gatagtatat attaatgtaa gtagtaaaaa tatttttgtt
     3661 tgtaaatatg agtattttta aacactataa atatgaatgt tataatatat acttatactt
     3721 ttctaataat
//
LOCUS       DAFNNB010000214         3703 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0219, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000214 DAFNNB010000000
VERSION     DAFNNB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0219"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(721..942)
                     /locus_tag="L3M60_003337"
     CDS             complement(721..942)
                     /locus_tag="L3M60_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227968.1"
                     /translation="MILKKVIISGICIFTCLLASSIPTFANELQYENIEQYATIEYAN
                     DSMERMSYIKSGSCGLTISNGIATIRSSV"
     gene            complement(1013..1726)
                     /locus_tag="L3M60_003338"
     CDS             complement(1013..1726)
                     /locus_tag="L3M60_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HBN6227969.1"
                     /translation="MEILSNKKDNFDITDDMLQEIIPIATKIILDDLPSDDELNHIFS
                     KSFERKMKKLIKQQKRSSLTNKIIFYSKRTAIVFLIVLASLFTITMSSEALRTRLYEL
                     VIRVYEDLTSFVFSTNDGEESLNLKIIEPKYIPNDFKEVDRVDNFIINYKNKNNIEIN
                     YMFCEISSNSIILDTENAKVENISINGNRAKYIEKGTSIQLFWNDKNCIYLLSTDCYK
                     EDLLKKYKNELIKMGKSIK"
     gene            complement(1710..2252)
                     /locus_tag="L3M60_003339"
     CDS             complement(1710..2252)
                     /locus_tag="L3M60_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6227970.1"
                     /translation="MLIYLAMLELEEDKIKFEKIYEKYRQIMFYVANKILKDDHLSED
                     AVHNAFLRIIKNIDKIDEVDSPRTKAFIVIIVERIAIDFYRKRKREKVSDIEEEYKNR
                     EVNFSIEDKVCESNLTIALAKLNESYFQVLSLKFQYGFSNKEIANVLNLGEENVYKRI
                     QRARKKLKEILEEMEDGNIE"
     gene            complement(2499..2681)
                     /locus_tag="L3M60_003340"
     CDS             complement(2499..2681)
                     /locus_tag="L3M60_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227971.1"
                     /translation="MKKRFYKVFLKTIPNLIIVILSLALSFYTNTFDIYLKILLLIPL
                     AYVLLFIYYYIKTKKD"
     gene            complement(2709..3320)
                     /locus_tag="L3M60_003341"
     CDS             complement(2709..3320)
                     /locus_tag="L3M60_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227972.1"
                     /translation="MKNKRINSILSIIMTTVIISLSFVTTANSMEKTESDNNIKIELS
                     DKEVEELHNYWPEDKIFSDEKIDEILIKTGQFTQEELDKINQEVDDYAKKVNQNSRVK
                     YINGYNKYRTFVKNGKKYLYIYVSGKTLKKIKAGADLSSTFGGFLPKIWVGLAVVVIG
                     KIITTNMNGINTNYGIVLSYIEDKWNFQGATYTYRYDHWFYQT"
     gene            3456..3650
                     /locus_tag="L3M60_003342"
     CDS             3456..3650
                     /locus_tag="L3M60_003342"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HBN6227973.1"
                     /translation="MQDLCKYTQNLSLKTTNQFLAAFLALVFSNNKVTNKVRQFLDLT
                     LKLIPYALVSTEYSEEKMNL"
BASE COUNT         1289 a          562 c          337 g         1515 t
ORIGIN      
        1 tgaaataact taaaacctca aaatatgtcc accttatacg ctatacttaa tattaagatt
       61 tttatactct ttataaagta ctgaaattta tagataaagt tttaaattaa tattaaagaa
      121 aataataact tattttttag gtcctttgtt cttatttctt ttgatatact cttttgctac
      181 tttatcaaat cgcccaagat aatccataaa aacatcacca gaagttaatt ttcttgaagt
      241 ataagaattt attactattt tcatttcaat cctcccatga attttattga tatatagtat
      301 tatagcgtgc ttctgattgt attcggattt ttttgaaatt tattaataac ttataagtac
      361 tttattcctt tctaaaaata tcaatcacaa gtataagtat tattattatt aataataaat
      421 tagcagactg gattaatata attggccatt caaaatatat tatttatagt cacctcatct
      481 tatatgtaca atatattttt attatctttt tttactactt attatatttt tgatttcaga
      541 gttagctttt accatagcat gaactctgta atatccttta ctgatttttt gttttttaga
      601 aattgaacaa gattattttc ttgaagcatt ccatgtatta attgtttacc atgtctttct
      661 attattatca ctcttttgta gtcttacaac tattgatgta ctttttacat tcactcttcc
      721 ctatacagaa cttcttatgg ttgcaattcc attagaaata gttaagccac aactaccaga
      781 ttttatataa ctcattcttt ccatagaatc atttgcatat tctatagttg catattgttc
      841 aatattttca tattgtaatt cattggcaaa tgtgggtatt gaagatgcca acaaacatgt
      901 gaaaatacat ataccactta taattacttt tttcagaatc ataaaaaaca cctcctcatt
      961 cttttgtata actataaaac gaataaagag atgctttctg gacactagaa aattatttta
     1021 tactttttcc catttttatt aattcatttt tgtatttttt caaaagatct tctttatagc
     1081 aatcagtact taataagtat atacaattct tatcattcca aaataactga atagaagttc
     1141 ctttttctat atattttgct ctattaccat ttattgatat attttcgacc ttagcatttt
     1201 ctgtatctaa tataatagaa tttgaactta tttcacaaaa catatagttt atttcaatgt
     1261 tatttttatt cttataatta ataataaagt tatccaccct atctacttct ttaaaatcat
     1321 ttggaatata tttaggttct ataatcttaa gatttaaact ttcttctcca tcattagttg
     1381 aaaaaacaaa agaagtcaaa tcttcatata ctcttattac caattcatat aaacgtgtac
     1441 gtaatgcttc gctactcata gtaatagtaa ataaactagc taaaactatt aaaaatacaa
     1501 tagctgttcg ctttgaataa aaaataatct tatttgtaag actacttctt ttctgctgct
     1561 taatgagctt tttcatcttt cgctcaaaac tttttgaaaa aatatgattt aattcatcat
     1621 ctgaaggaag gtcatcaaga ataatttttg tcgcgattgg gataatttct tgaagcatat
     1681 catccgtaat atcaaaatta tcttttttat tactcaatat ttccatcctc catttcttct
     1741 aatatttctt ttaatttttt tctagcacgc tgaatccttt tatatacatt ctcctctcct
     1801 aaatttaata catttgctat ttctttattt gaaaaaccat actggaattt taaagaaagt
     1861 acttgaaagt aactttcatt aagttttgca agagctatag ttaaattact ttcacatacc
     1921 ttatcttcta tagaaaaatt tacctctcta tttttatatt cttcctcaat atcagatacc
     1981 ttttcccttt ttctctttct gtaaaagtct atagcaatcc tttctactat tataacgata
     2041 aaagccttcg ttcttggact atctacctca tcaattttat ctatattttt aattattctt
     2101 aaaaaagcat tatgtactgc atcctctgat aaatgatcat cctttaatat tttattggcc
     2161 acataaaaca ttatttgtct atacttttca tatatctttt caaactttat tttatcttct
     2221 tcgagttcaa gcatagctaa ataaattaac atttagaatt cctccaatat aaaaattctg
     2281 aattaatatt ttaaagttca attataacat aaatatataa ttaactctac ttaaatagaa
     2341 tataattatg cttaacaagc tttttaaata tacctagtaa aaaggccctt caaatccatt
     2401 ttggggatta taagggcctt aactttacaa ctaattctca aagttaaact tttaaattaa
     2461 ttattttcgt atatatctaa ttccataaaa aataaaaatt aatccttttt tgttttgatg
     2521 taatagtata taaacaaaag tacatatgct aaaggtatta aaagcaatat ttttaaataa
     2581 atatcaaatg tattagtata aaatgataat gcaagtgata atatcacaat tatcaggttt
     2641 ggaattgttt ttaaaaaaac cttatagaat ctttttttca tattattcct cctaaaaaag
     2701 catgtctttt aagtttgata gaaccaatgg tcatatctat aagtatatgt tgcaccttgg
     2761 aaattccact tatcctctat gtatgataaa acaatcccat aattagtatt tataccattc
     2821 atattagttg ttattatctt tcctataact actacagcaa gacctaccca aatttttggc
     2881 aaaaaacctc caaaagtaga tgaaaggtct gctccagctt ttattttctt taaagtctta
     2941 ccacttacat aaatatataa atatttctta ccatttttga caaaggttct gtacttgtta
     3001 tatccattta tatactttac acgagaattt tgattgactt tcttagcata atcatcaact
     3061 tcttgattaa ttttatccaa ttcttcttga gtaaattgac cagttttaat taatatttca
     3121 tcaatttttt catctgaaaa aattttatct tcaggccaat aattatgcaa ctcctctact
     3181 tctttatcag acaactctat ttttatatta ttatctgatt ctgttttttc cattgaattt
     3241 gctgtagtaa caaatgataa agatattatc acagttgtca ttattatgga taaaatgcta
     3301 ttaattcttt tattcttcat ttaaatcctc ctaactataa taaatttata aaccttgtac
     3361 atatataacg tatctaaact cgattattgg acaaaaattt taaaaatatt tatattttta
     3421 aaattaaatc ttctagaaac tttaaatgta aatggatgca agatttatgt aagtatacac
     3481 aaaatttatc attaaaaact actaatcaat ttctagctgc tttcttggca ttagtatttt
     3541 ctaataataa agtaacaaat aaggttagac aatttttaga cttaacatta aaactaatac
     3601 cttatgccct tgtatctaca gaatatagcg aagaaaaaat gaacttatag atagatttga
     3661 taaaaagaaa atacatataa atatataact tttagtgatg taa
//
LOCUS       DAFNNB010000215         3691 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0220, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000215 DAFNNB010000000
VERSION     DAFNNB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0220"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            76..354
                     /gene="spoVG"
                     /locus_tag="L3M60_003343"
     CDS             76..354
                     /gene="spoVG"
                     /locus_tag="L3M60_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003421453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HBN6227974.1"
                     /translation="MKITDVRVRKLTEEGKMKCIVSITFDNLFVVHDIKVIEGHNGLF
                     IAMPSRKVGEGNFRDIAHPINAEMRQVLEDAVLQAYHEALVQWEVAAE"
     gene            567..1946
                     /gene="glmU"
                     /locus_tag="L3M60_003344"
     CDS             567..1946
                     /gene="glmU"
                     /locus_tag="L3M60_003344"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBN6227975.1"
                     /translation="MNFKAIILAAGKGTRMKSKYPKVIHKVCGKEMVNHIIDVSKKSG
                     VKDTVVILGHEADVVKEKLAEEIIIAMQTEQLGTGHAVKMAKEYINDEDTIVVLCGDT
                     PLIKEETLKRLFEYHIENKYHATVLTTRVGNPTGYGRIIRDKKGDLLKIVEQKDANSE
                     EKMISEINSGIYCFNGKSLREALDLLNNNNSQGEYYLTDTAKIMRDKGLKVGAFAGST
                     IEELMGVNSRVELSKAEEIMRRRINESHMVNGVTIIDTNSTYIESDVIIGNDTIIYPG
                     VMLQGKTKIGSDCIIGMNTSITNSEIGDGTEVKNSTIIDSKVGENSTVGPYAYLRPKS
                     DLGNNVKIGDFVEVKNAIIEDGSKASHLSYIGDAHVGKNVNIGCGVVFVNYDGKNKFK
                     SIVKDNAFIGSNSNLVAPVVVEEKGYIATGSTITHNVPDGALAIARERQVIKEGWVEK
                     KNQKDNQSK"
     gene            2040..2990
                     /locus_tag="L3M60_003345"
     CDS             2040..2990
                     /locus_tag="L3M60_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HBN6227976.1"
                     /translation="MNTSGSEIKIIAGNSSKELAQKIADYIGVSVLDCEVGTFSDGEI
                     CVNMNETVRGCDVFVVQSTNSPVNDNLMELLILIDALKRASAGRITAVIPYYGYARQD
                     RKAKARDPITAKLVANLITAAGADRVLTMDLHAAQIQGYFDIPLDHLLGGTILANYFN
                     EKKIEDLVVVSPDLGSVTRSRKFANTLNGEVPIAIIDKRRPKANVCEVMNLIGDVKGK
                     NVILLDDMIDTAGTIVNAANALKEFGAKDVYACCTHGVLSGPAIERIANSEISELIVL
                     DTIQLPEEKRIDKIKIKTVAPLFGDAIRMIFSNESVSKLF"
BASE COUNT         1494 a          366 c          691 g         1140 t
ORIGIN      
        1 tatattaatt tattccagcg ataataattt taatataaat taatttctgt caaaatcaaa
       61 gggggatatt ttggtatgaa gataactgac gtaagagtaa gaaaattaac agaagaaggt
      121 aagatgaaat gtatagtttc aataactttc gataatttgt ttgtagtgca tgatataaaa
      181 gttatagaag gccataatgg gctatttata gcaatgccta gcagaaaagt aggcgaagga
      241 aatttcagag atatagctca tccaataaat gcagaaatga gacaagtttt agaagatgca
      301 gtattacaag catatcatga agcattagtt caatgggaag tagctgctga ataataataa
      361 atatataaaa tggagagagc ccgaacattt aggctctttt taaatgtaga atttctatta
      421 aaatatgtac tttttagtaa aaaagtgcta tattattagt gataatgtat atgttgtata
      481 caaattgtta gataaatgta ttataatgta tattgattaa gagaataata cgaataaaat
      541 taaattagat tgaatggagt gtatcaatga actttaaagc tataatcctt gctgctggca
      601 aaggaacaag aatgaagtct aaatacccaa aagttataca taaagtatgt ggaaaagaaa
      661 tggttaacca tattatagat gtttcaaaaa aatcaggggt aaaagatact gtcgtgattt
      721 tagggcatga agctgatgta gttaaagaaa agcttgcaga agaaattata atagccatgc
      781 aaacagaaca gcttggaaca ggacatgctg tgaagatggc taaagaatat attaatgatg
      841 aagatacaat agttgtactt tgtggagata ctccacttat aaaagaagag actctgaaga
      901 gactatttga atatcatata gaaaataaat atcatgctac agttcttaca acaagagttg
      961 gaaatccaac aggttatggt aggataataa gagataagaa gggtgatctt cttaaaatag
     1021 tggaacaaaa agatgcaaat tcagaagaaa aaatgataag tgagataaac tcaggaatat
     1081 attgttttaa tggaaagagt cttagagaag cactagattt gctaaataat aacaattcac
     1141 aaggtgaata ttatttaact gatactgcta aaataatgag agataaagga ttaaaagttg
     1201 gtgcatttgc aggttcaact attgaagaac ttatgggcgt taattcaaga gtagaattat
     1261 caaaggcaga agaaattatg agacgaagaa taaatgaatc tcatatggta aatggagtga
     1321 ctataataga cacgaactct acctatatag aatctgatgt tataataggt aatgatacca
     1381 taatatatcc aggtgttatg ttacaaggaa aaacaaagat aggttcagat tgtataattg
     1441 gaatgaatac atctataaca aattcagaaa taggtgatgg tacagaagta aaaaactcaa
     1501 ctataattga tagtaaagtt ggagaaaatt ctactgttgg accatatgca tatctaagac
     1561 caaagagtga cttaggaaat aatgtgaaaa taggcgattt tgtagaagta aaaaatgcta
     1621 ttatagaaga tggctcaaaa gcatctcatc tttcttatat aggagatgca catgtcggca
     1681 aaaatgtaaa tattggatgt ggagtagtat ttgtaaacta tgatggtaaa aataaattta
     1741 aatcaatagt aaaagacaat gcgtttatag gttcaaattc taatttagtt gcaccagtag
     1801 ttgtagaaga aaaaggatat atagcaacag gttcaacaat aacacataat gttccagatg
     1861 gtgctttggc aatagcaaga gaaagacaag ttattaaaga aggttgggta gaaaaaaaga
     1921 atcaaaaaga caatcaaagt aagtagttat gtagtttatt attttggcta aaaatttagc
     1981 tcgaaattat tataaattat attcattagt attttaaaat tttcaggagg gttacataaa
     2041 tgaatactag cggaagcgag attaaaatta ttgcaggtaa ttcatcgaaa gagttagcac
     2101 aaaaaatagc tgactatata ggtgtatcag tactagattg tgaagtaggt acatttagtg
     2161 atggtgaaat atgtgtaaac atgaatgaga cagtaagagg ttgtgatgtg tttgtagtgc
     2221 aatctactaa cagcccagta aatgataact taatggagtt attaatctta attgatgcat
     2281 taaagagagc atcagcaggt agaataacag cagttattcc ttactatgga tatgcaagac
     2341 aagatagaaa ggctaaggca agagatccaa tcactgctaa attggttgca aacttaataa
     2401 ctgctgctgg agcagataga gtattaacaa tggatttaca tgcagctcaa atacaaggat
     2461 actttgatat accattagac cacttactag gtggaacaat attagcaaat tattttaatg
     2521 aaaagaaaat agaagattta gtagtagtat ctcctgactt aggaagtgtt actagatcaa
     2581 gaaaatttgc taatacttta aatggagaag ttccaatagc tataatagat aaaagaagac
     2641 caaaagctaa tgtatgtgaa gttatgaact taataggtga tgttaaaggt aaaaatgtta
     2701 tactattaga cgatatgata gatacagcag gaacaatagt taatgctgca aatgcactta
     2761 aagagtttgg agcaaaagat gtttatgctt gttgtactca tggtgtgtta tcaggcccag
     2821 ctattgaaag aattgctaat tcagagataa gtgagttaat agttcttgat actatacaac
     2881 ttcctgaaga gaaaagaata gataagataa agataaaaac agtagctcct ttatttggag
     2941 atgctataag aatgatattc tctaatgaat cagtaagtaa attattctaa tttattgtat
     3001 ttatgagagt tgtttaaaag gatataagcc ttttgagcaa ctctttttga tttacttatg
     3061 gaatgttatt tttctcttgt tagaaaggta tctattattg tagaaagaga ttatagttta
     3121 aaatttatgt atatttaatc aaaataaatt taataaaaga tatattattc aaaatattat
     3181 gaatataaaa aatattacaa aaaagtattg aaaaaattca gattatgtag tattatgtaa
     3241 atataaataa agaaagaggt gtttccaatg ggaaggttag aagatgaacc cctaataatt
     3301 aaatgattca gtcacaatct aaagtgaata taaaaatcta ttgaaagttt atttataaga
     3361 attgaaaata gtatctattc tatctttagt gtttgtgaga gggttctgga ttatataaaa
     3421 ttactgtaaa gaaagtaatt ttgcacaata agcatatctg taaatgaaga gtttcgttta
     3481 tataatgatt gtggctgaat cataactata atagatagaa tgtttataat agatgatttt
     3541 ataaaatact atattgtaat agcttttagc tttcaaataa atattagcaa aggatggaaa
     3601 tgacaatgat aaaactaaat gataatttat aaatatctcc ttacgatata taataattgt
     3661 agggagatgt ttcttttttt gtatagtata a
//
LOCUS       DAFNNB010000216         3625 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0221, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000216 DAFNNB010000000
VERSION     DAFNNB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0221"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            491..1063
                     /locus_tag="L3M60_003346"
     CDS             491..1063
                     /locus_tag="L3M60_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227977.1"
                     /translation="MNENLSKEIKILGTIEVEEMKFHDIEGGFGVGKKSMSVKDIDEI
                     HNGKLKDINKNINNNRKRFKDMINTLDLKVSDLKSLSLEMGYSNQSYANANNIYLLPE
                     RDYSKRLKILEGKQYEKIVDGYFSMRKELNNPLLSASKELQAIFMLDKKQEFLETKIE
                     SVNEKLENFMDDAPLFNIEFESIVKIVLWK"
     gene            1506..1877
                     /locus_tag="L3M60_003347"
     CDS             1506..1877
                     /locus_tag="L3M60_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227978.1"
                     /translation="MKKQIMALGLTGLIGLGGLGLLANKSFADTPNKLKGDTTVAQGL
                     NKNATAKDVTEQVDTTIGKGLDKNAIVKDVVENNGEKSDTTVAPGLDKNATVKDTAEK
                     GNTTIAPGLDKNTIVKDIVVK"
     gene            2016..2273
                     /locus_tag="L3M60_003348"
     CDS             2016..2273
                     /locus_tag="L3M60_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227979.1"
                     /translation="MVKCTKLIGGVITMKKNILGVLLPLLTMIIIFVLSFENIITNSL
                     GLSDMDIKGILISGVVLYIPVSFLVNGIICAKKQIKWKISK"
     gene            2596..2880
                     /locus_tag="L3M60_003349"
     CDS             2596..2880
                     /locus_tag="L3M60_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227980.1"
                     /translation="MTFEVEFYTFEGINMEAEMYVQVAMMASKVVKITGVDEVIKKLV
                     KKFLNNKVEEALGKQRSKKELKLIENNFNEYMERSYKNYIYMNTIVFRNQ"
     gene            complement(3157..3393)
                     /locus_tag="L3M60_003350"
     CDS             complement(3157..3393)
                     /locus_tag="L3M60_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227981.1"
                     /translation="MAPNFNVENIVLEDKEIVSTPQKYVLISRYYDNGKTIAFIEMNS
                     TKYKTEYIRNFDQYVDEFSTLEETEECLQECLSA"
BASE COUNT         1453 a          383 c          557 g         1232 t
ORIGIN      
        1 gatactcata catataaaaa aacaataatt cagtatttta aattttaata gttgtttttt
       61 attatcattg attgccataa gtactcctaa aattatagat agtatgataa atgtatttga
      121 tgcttgcaaa taagaaaaga tagtcataaa tttattctcc taatattaac tataaattgt
      181 aaaaaaataa tttaaataac taacaattga ataaccaata aaaaatgtaa ataaataaaa
      241 aagaaacttt ttaaatccat ttaatttaaa tcttttttct atcttttgac aaatgaaatt
      301 tatcggaata aaaacaaaag tcgcaattaa aaaagttatt aaatactttg acataggata
      361 atagtccata aataatcacc tcataaatta gtataaccat agattaatat gagttgttaa
      421 taatagcaat caatttttca gaactgtaaa taaattgaaa taaattatcc aaaaaaaaga
      481 gcaatagact atgaatgaaa atttaagtaa agaaattaaa atactaggaa ctatagaagt
      541 tgaagaaatg aaattccatg acattgaagg tggatttgga gtaggtaaga aatcgatgtc
      601 agtaaaagat atagatgaga tacataatgg aaaactgaag gatataaata aaaatataaa
      661 taataataga aaaagattta aagatatgat aaatacatta gatttaaaag tgagtgattt
      721 aaaatcactg agtttagaaa tgggttactc taatcaatct tatgcaaatg caaataatat
      781 ttacttatta cctgagagag attattcaaa acgactaaag atattagaag gtaaacaata
      841 cgagaaaata gttgatgggt atttctctat gagaaaagaa ttaaataatc ctcttttaag
      901 tgcatcaaag gagttacagg ctatatttat gttagataag aaacaagaat tcttagaaac
      961 taaaatagaa agtgttaatg agaaattaga gaattttatg gatgatgcac cactattcaa
     1021 tattgagttc gagagtattg taaaaatagt attgtggaaa tgaaaatttc cctatcaaaa
     1081 ctaaataatt ggctagggtt aagggattac cctagtcact atgaacagac taggcagggc
     1141 gtgaggacgc tattagttca attctaacta tgttcaaaat cattgataca ctatattaag
     1201 tatctgaatt gagactaaaa ttcatacagt tttgtatctt aattcagaat ctgaaaattg
     1261 agtggggttt ggcaacctca ctaaccatgc agatactggt atttaatcta agttcgattc
     1321 ttaggacctg ctacataata tgaatctccc tctttataaa atgactaagt tcgggtaagc
     1381 ttagtctttt attttttaaa caagaaaatg agaatttgga ttaattagca ttgtaaacaa
     1441 tcagtaaaca ttaatatgat attatttgtt tagaaaataa aacaaagaaa aaggagagaa
     1501 taattatgaa aaaacaaatt atggctttag gattaacagg attaattggc ttaggaggtc
     1561 ttggattact tgcaaataaa tcatttgcag atactcctaa taaacttaaa ggagatacaa
     1621 cagtagctca aggattaaat aaaaatgcaa ctgctaaaga tgttacagaa caagtagata
     1681 caacaatagg taaagggtta gataaaaatg caattgttaa agatgttgta gaaaataatg
     1741 gagaaaaaag tgatacaaca gtagctccag gtttggataa aaacgcaact gttaaagata
     1801 ctgcagaaaa aggcaataca acaatagctc caggattaga taaaaacaca attgttaaag
     1861 atattgtagt aaaataattt agtttttata gctggcaaaa gggtggaaat ttattctgcc
     1921 cttttgtttt attaataaaa atttacatat attaaatatg gatagcaaag acttagatta
     1981 atttctagct cttttatatt ttaaaggttg tgtttatggt aaaatgtact aaactaattg
     2041 gtggggtgat aactatgaag aaaaatattt taggagtatt gctaccatta ctaacaatga
     2101 ttataatatt tgttctatca tttgaaaata taataactaa tagtttagga ttaagtgata
     2161 tggatataaa aggaatcctt atttcaggtg tagtattgta tataccagtt tcatttttag
     2221 ttaatggtat aatatgtgct aaaaagcaaa taaagtggaa aattagcaaa taaagtggaa
     2281 atttccagtt ttaatatcat ttttaggctc agttgttatt atgatgtatc taagttttga
     2341 tagcattgaa atggcattgt atttatcata ttattttata tcatattata ttggatattg
     2401 gataactagt gcaatgaaga aataaaatta aggtgtaaat ataaaaaata gaataagttg
     2461 agagactatc tttataaatg gtcttttttt atacaatgaa tcaaaaatga aatatattta
     2521 tgcattatgg agagaaatat ggtaaaagta ttatttggaa ttattttgta gttatagaat
     2581 agtttttgaa ggatattgac ctttgaagtt gaattttata cttttgaggg gattaatatg
     2641 gaagcagaaa tgtatgtgca ggtagctatg atggcaagca aagttgtaaa aataactggt
     2701 gtggacgaag tgataaaaaa attagttaaa aaatttttaa ataataaagt agaagaagcg
     2761 cttggaaaac aaaggagtaa aaaagaatta aaattaatag aaaataactt taatgaatat
     2821 atggaaagaa gttataaaaa ttatatatac atgaatacta tagtatttag aaatcaataa
     2881 aaacaattga tgatttatat ataccattaa cagtttcaaa gcgtaatatt tttgatgctt
     2941 cttgtgaaga agatgtaact gatattttaa tcaatagtta taaaagatga ctttttacca
     3001 aagtacaaaa agtttttgtt gatagaccaa gcaggtatgg gaaaatccga ctatagctaa
     3061 atgattatct aagtcaaaac ttctatttat ctctagttgt ttgttattta caagtctttt
     3121 cagtatataa gtcttaaaac acatttatat caccccttaa gcactaagac attcttgcaa
     3181 gcattcctct gtttcctcta atgtagaaaa ttcatcaaca tattggtcga agtttctaat
     3241 atattcagtc ttatatttag tgctattcat ttctataaaa gcaatagttt taccattatc
     3301 ataatatctg cttattaaga catatttttg tggggtagat acaatttcct tatcttctag
     3361 gactatgttt tcaacattga aattaggtgc catgctatag ttacctactc ttatgtattc
     3421 ataatcatca ctgaaacgga ttttatcacg tctaacagag tcatcttctt tataagttat
     3481 aagttttatc agttctttta ataacttcta tcgtgtatat gcagtcaagg tcacatatac
     3541 ttcttgttgc ataagtttta cctacttcaa attttatcat tttaaataca tccaataatt
     3601 aatttgttaa gtttattgta cacta
//
LOCUS       DAFNNB010000217         3602 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0222, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000217 DAFNNB010000000
VERSION     DAFNNB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0222"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            157..1593
                     /locus_tag="L3M60_003351"
     CDS             157..1593
                     /locus_tag="L3M60_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein Cwp28"
                     /protein_id="HBN6227982.1"
                     /translation="MKFYKRILTLILSISFVFANIQLANAISSVEKIQGKNKYEIAGK
                     IADKTAYKTAILINTSNSIADGLSASGLAGALNAPILLTEKNTIPTETSARLKNVSKV
                     YIIGGTYSISTSVENSLKSKKMKVVRIKGNDRIKTSYNVAKEINSIKKVNTVMLTNAY
                     KGEADAISIASVAARDKSPIILTNGQSIPFSTSGLKSYAIGGTASMSTTLVNNTKSTR
                     LGGSTRFETNKAIINKFYKDAREFYIAGAYELTNALVGSSLSKHEPMVLVNDGSNKSV
                     LKNAKKITSIGYIDSNIVQQCLNITNGIGDINTGIVKNIKPTTRTIKDGMYKVGKDIS
                     AGEYLITSNSGSYDSYYEVTSDSTGNADSILSNDIFSGTRYITLKNGQYIKIEDSTMI
                     LAKYAKAQKAKNGKFGNGMYKIGLEIPAGEYIIMSNSSDAYYEVRNNSLGNAEGIVTN
                     DTFSGRRYITVEEGQYLILNDCYLIENE"
     gene            1844..3280
                     /gene="miaB"
                     /locus_tag="L3M60_003352"
     CDS             1844..3280
                     /gene="miaB"
                     /locus_tag="L3M60_003352"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBN6227983.1"
                     /translation="MSKRKQVTIPIEKIQEQDKYIEQIHRQNEEYFNRTGKRKLVFTQ
                     TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLK
                     HLKGKNPNMKIAVCGCMMQQPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESMNSDK
                     MLVDVWDVDGEVIEGLRSNRKFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDI
                     INEIKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERIRFMTSHP
                     KDISDEVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPE
                     IAFSTDLMIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDI
                     KHERFNRVLEAVNRISAEINDGYKNRIVEVLVEGRSKNNENKLSGRTRQNKLVNFEGG
                     NDDLIGKLVMVEITEPRTFSLNGILVND"
BASE COUNT         1501 a          350 c          596 g         1155 t
ORIGIN      
        1 taaatacaat tataactaaa tgaataattg tatttatatc tattggtaac atggtctaat
       61 ataatttgta attttacata agtttgttaa gtacataact aacctaagag tatgtattta
      121 tatattaaaa ttttattctg gggggacaat tttattatga aattctataa aagaatatta
      181 acattgattt taagtatttc ttttgtattt gcaaatattc aactagcaaa tgcaatatca
      241 tctgtagaaa aaatacaagg aaaaaataag tatgaaatcg caggtaagat agcagacaaa
      301 actgcatata aaactgctat attaattaat actagtaact ctatcgcaga tgggttaagt
      361 gcaagtggac tagcaggagc tttaaatgcc cctatactat taactgaaaa aaatactata
      421 ccaactgaaa cgtctgcaag attaaaaaat gtatcaaaag tttatataat tggaggaaca
      481 tattctatta gtacatctgt cgaaaattca ttaaaatcta agaaaatgaa agttgtaaga
      541 attaagggaa acgatagaat aaaaacaagt tataatgttg caaaagaaat aaattcgata
      601 aaaaaagtta atactgttat gttaaccaat gcatataaag gagaagcaga tgctataagt
      661 attgcatctg tagcagcaag agataaatca ccaataattc ttactaatgg acaaagtata
      721 cctttttcaa ctagtggttt aaagtcttat gcgataggag gaacagcttc aatgagtact
      781 actttagtaa acaatactaa atctaccaga ctaggaggga gtactcgttt tgaaactaat
      841 aaagcaataa taaataagtt ttataaagat gcgagagagt tttatatagc tggagcttat
      901 gaattaacaa atgcacttgt aggttcttct ttatcaaaac atgagccaat ggttttagtt
      961 aacgatggta gcaataaaag tgttttaaaa aatgctaaga aaataacttc cataggatat
     1021 atagattcaa atatagtaca acaatgttta aatattacaa atggaattgg agatattaat
     1081 actggaattg taaaaaatat taagcctact acaaggacta taaaagatgg aatgtataag
     1141 gttggaaaag atatttcagc aggtgaatac ttaataactt caaattcagg aagttatgat
     1201 tcttattatg aagttacaag tgatagtaca ggtaatgctg atagtatact ttcaaatgat
     1261 atttttagtg ggactagata tatcacttta aagaatggtc aatatattaa aattgaggat
     1321 tcaacaatga tattagcaaa atatgctaaa gcccagaaag ccaagaatgg taagtttgga
     1381 aatggtatgt ataaaattgg attagaaatt cctgctggag aatatattat aatgtcaaat
     1441 tcttctgatg catattatga agttagaaat aatagcttag gtaatgcaga aggaatagtt
     1501 actaatgaca ctttttcagg aagaagatat ataacagttg aagaaggtca atatttaata
     1561 ttaaatgatt gttatttaat tgaaaatgaa tagatgtaat tgtatttaat agtaagtaat
     1621 atattttata taagaaagga tgttttaagt tttaatttaa aacatctttt tttattatta
     1681 taataaatta aatgattttt atattttaga agtatagtat atataaatca ttaaatttac
     1741 tattaaaaca cactagaaaa ataataatat ctttaaaaga tttttcttat ttgatataat
     1801 taaggttatg tgtaatataa gaaattaact aggaggaata gaagtgagca aaagaaaaca
     1861 agtaacaata cctatagaga aaatacaaga acaagataaa tatatagaac aaatacacag
     1921 acaaaatgag gaatacttca acagaactgg aaagagaaaa ctcgtgttta ctcaaacctt
     1981 tggatgccaa atgaatgaac atgattcaga aaaattatgt tcaatgttag aagagatggg
     2041 atatcaaatg tcaatgatgg tcgaagaaag tgatttaata atatataaca catgtgcagt
     2101 tagagaaaat gcagagttaa aagtgtatgg taatttagga caattaaaac acctaaaagg
     2161 aaaaaatcca aatatgaaaa tagctgtatg tggatgtatg atgcaacaac cacatgttgt
     2221 agaggaatta agaaaaaaat ataaacatgt agaccttata tttggaacac ataatctata
     2281 taaatttcca caattgctta cagaatcaat gaattctgac aaaatgttag tagatgtatg
     2341 ggatgtggat ggagaggtaa tagaaggtct tagaagtaat agaaaatttg aattaaaagc
     2401 atttgtaaat ataatgtatg gatgcaataa tttctgcacg tattgtatag taccatacac
     2461 aagaggaaga gaaagaagta gaactcctga agatataatt aacgagataa aagagcttgt
     2521 tgcaaatgga acgaaagaaa taactttact tggacaaaat gtagattctt atggtaagac
     2581 acttgaaaat ccagtttcat tctcagaact tttaagaaaa gtgaatgata tagaaggaat
     2641 tgaaaggata cgatttatga catctcatcc aaaagatata tctgatgaag taatatatgc
     2701 aattagagat tgtgataagg tgtgtgagtt tttacattta ccaatacaat gtggaagcag
     2761 tagtcttttg aaaaaaatga atagacatta tacaaaggaa tattatttag aaataataga
     2821 aaaagctaaa aaagaagttc cagagattgc tttttctaca gatttaatga ttggtttccc
     2881 aggagaaaca gaagaagatt tattggatac tttagatgta gttgaaaaag ttagatatga
     2941 ttcagctttt acatttattt attctaaaag acagggaact cctgctgcta aaatggaaaa
     3001 tcaaatacca gaagatataa agcatgagag atttaataga gtattagaag ctgtaaatag
     3061 gatttctgca gaaataaatg atggatataa aaatagaata gttgaagttc tagttgaagg
     3121 aagaagtaaa aataatgaaa ataagctttc tggtagaact agacaaaata aacttgtaaa
     3181 tttcgaaggt ggaaatgatg atttaattgg taagttagtt atggttgaaa ttactgaacc
     3241 tagaactttt tcattaaatg ggatactagt aaatgattaa tattgtataa aataataaaa
     3301 ttatgtaaac taatcgaaat agatgtacta tagaacaaat tatattaaaa atttttattt
     3361 taagcgagtt tgtcatatgt tatgctaatt taagtttttg ctattaataa aaataaactt
     3421 gatataagat ataatttcat tgtacatcta tttttgtttt attatatttt atcaaagcct
     3481 acttaattgt cattagtagt tttctgttat tatattaaat tataatatgg tagactttaa
     3541 acacaaagat aaaattatta ttagaagttt atactatata cattattttc atatagattt
     3601 tc
//
LOCUS       DAFNNB010000218         3582 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0223, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000218 DAFNNB010000000
VERSION     DAFNNB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0223"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..358)
                     /locus_tag="L3M60_003353"
     CDS             complement(<1..358)
                     /locus_tag="L3M60_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HBN6227984.1"
                     /translation="MILKKHKETILYLFFGAFTTLVNIVSYLFFTRVISFNFMVANAL
                     AWILAVLFAYVTNKFFVFESKRIELRFLFKEFFSFVSFRLLSGVIEMVIMYIMIDLLF
                     VNDIIVKVFTNIVVIVL"
     gene            586..2997
                     /locus_tag="L3M60_003354"
     CDS             586..2997
                     /locus_tag="L3M60_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding cysteine protease Cwp84"
                     /protein_id="HBN6227985.1"
                     /translation="MRKYKSKKLSKLLALLTVCFLIVSTIPVSAENNKTLDGVETAEY
                     SESYLQYLEDVKNGDTAKYNGVIPFPHEMEGTTLRSKSRSSLPSSYKSSVAYNPMNLG
                     LTTPAKNQGSLNTCWSFSGMSTLEAYLKLKGYGTYDLSEEHLRWWATGGKYGWNLDDM
                     SGSSNVTAIGYLTAWAGPKLEKDIPYNLKSEAQGATRPSNMDTAPTQFNVTDVVRLNK
                     DKETVKNAIMQYGSVTSGYAHYSTYFNKDETAYNCTNKRAPLNHSVAIVGWDDNYSKD
                     NFASDVKPESNGAWLVKSSWGEFNSMKGFFWISYEDKTLLTDTDNYAMKSVSKPDSDK
                     KMYQLEYAGLSKIMSNKVTAANVFDFSRDSEKLDSVMFETDSVGAKYEVYYAPVVNGV
                     PQNNSMTKLASGTVSYSGYINVPTNSYSLPKGKGAIVVVIDNTANPNREKSTLAYETN
                     IDAYYLYEAKANLGESYILQNNKFEDINTYSEFSPCNFVIKAITKTSSGQATSGESLT
                     GADRYETAVKVSQKGWTSSQNAVLVNGDAIVDALTATPFTAAIDSPILLTGKDNLDSK
                     TKSELQRLGTKKVYLIGGEDSLSKNVQAQLSNMGISVERISGSDRYKTSISLAQKLNS
                     IKSVSQVAVANGVNGLADAISVGAAAADNNMPIILTNEKSELQGADEFLNSSKITKSY
                     IIGGTATLSSNLESKLSNPTRLAGSNRNETNAKIIDKFYPSSDLKYAFVVKDGSKRQG
                     DLIDGLAVGALGAKTDSPVVLVGNKLDESQKTVLKSKKIETPIRVGGNGNESAFNELN
                     TLLGK"
     gene            3300..>3582
                     /locus_tag="L3M60_003355"
     CDS             3300..>3582
                     /locus_tag="L3M60_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6227986.1"
                     /translation="MKAPKTILTILTIALTLSSISIIPSYALTEEKLVGNGRYETAVK
                     ISQKAYSSSSNVVLVNDNSLADALSATPFAKAKGAPILLTESDKLDDRTE"
BASE COUNT         1475 a          491 c          572 g         1044 t
ORIGIN      
        1 ttaaaactat aacaactata ttagtaaata cttttacaat aatatcattt acaaaaagta
       61 aatcaatcat tatatacata ataaccattt ctataacacc agaaagtagc ctaaaactaa
      121 caaaagaaaa aaattcctta aacaaaaatc ttagttcgat tcttttactt tcaaacacaa
      181 aaaacttatt agtaacataa gcaaataaaa cagcaagtat ccatgccaaa gcatttgcaa
      241 ccataaaatt aaatgaaata actctagtaa aaaataaata cgaaactata tttacaagag
      301 tagtaaatgc tccaaaaaat aaatataata ttgtctcctt atgctttttt aaaatcaaat
      361 ataatcctcc taagctaatt taatataaat atacttaaag atactaacaa acattagtat
      421 agcacatcta tcaataaatc aatatatctt aaaatattat aaaaaataaa actataatat
      481 tctataatta atacgtactc ataatccata ttttatcaaa ataaaatatg accttaattt
      541 agaaatctga tataataaaa attaaataca tataaggggg taaacatgag aaagtacaaa
      601 tcaaaaaaat tgtcaaagct actggcattg ttgacagttt gctttttaat agtgtcaaca
      661 atacctgtct cagcagaaaa caataaaact ctagatggag tagaaactgc agagtattct
      721 gaaagctatc ttcaatacct agaagatgtc aaaaatggag acacagcaaa atataatgga
      781 gtaatacctt ttccacatga aatggaaggt acaacacttc gaagtaagag tagaagtagt
      841 cttccatcat catacaaatc aagtgtagca tacaacccaa tgaatcttgg tcttactaca
      901 ccagcaaaaa atcaaggaag tcttaataca tgctggtctt tttcaggtat gtcaacttta
      961 gaagcatatc ttaaattaaa gggatatgga acgtatgact tatcagaaga acatttaaga
     1021 tggtgggcaa ctggtggaaa atatggatgg aacttagatg atatgtcagg tagttcaaat
     1081 gtaacagcca taggatatct gacagcatgg gcaggtccta agttagaaaa agatatacca
     1141 tataatctta aatctgaggc gcaaggtgca actagacctt caaatatgga tactgcacct
     1201 acacaattta atgtaacaga tgttgttcgt cttaataaag ataaggaaac tgtaaaaaat
     1261 gctataatgc aatatggttc agtgacatca ggttatgcac attattcaac atattttaat
     1321 aaagatgaaa cagcatataa ctgtacaaat aagagagctc cattaaatca ctctgtagcg
     1381 atagtaggat gggatgataa ttattcaaaa gataactttg catctgatgt aaaaccagaa
     1441 tcaaatggag catggttagt aaaaagcagt tggggagagt ttaattctat gaaaggattc
     1501 ttctggattt cttatgaaga taaaactcta ctaacagata cagataacta tgcaatgaaa
     1561 tcagtatcaa aaccagatag tgataaaaaa atgtaccaac ttgaatatgc tggtcttagc
     1621 aagataatgt caaataaagt aacagcagca aatgtatttg attttagcag agattctgaa
     1681 aaacttgatt ctgttatgtt tgaaacagac tctgtaggag caaaatatga agtatattat
     1741 gcaccagtag taaatggagt tcctcaaaat aattcaatga caaaacttgc aagtggaaca
     1801 gtatcatatt ctggatacat aaatgtacct actaattctt acagcttacc aaaaggtaaa
     1861 ggagcaatag tagtagttat agacaacaca gcaaatccta atagagaaaa atcaacttta
     1921 gcatatgaaa ctaatataga tgcatattat ttatatgaag ctaaagcaaa cttaggtgaa
     1981 agttacatac ttcaaaacaa taaatttgaa gatataaata catatagtga attttctcct
     2041 tgtaactttg ttataaaagc tataacaaaa acatcttctg gacaagctac ttcaggagaa
     2101 tctttaactg gagcagatag atatgaaaca gcagttaaag ttagtcaaaa aggatggact
     2161 tcttcacaaa atgcagtatt agtaaatgga gacgcaatag tggatgcttt aacagctaca
     2221 ccatttacag cagcaatcga ctctccaatt cttttaacag gaaaagataa cttagattca
     2281 aaaactaagt cagagttaca aagattagga actaaaaaag tttatctaat aggtggagaa
     2341 gattctttaa gtaagaatgt acaagctcaa cttagcaata tgggtatatc agtagaaaga
     2401 atttcaggta gtgatagata caagactagt atatctctag ctcaaaagct aaatagtata
     2461 aaatctgttt cacaagttgc agtggcaaat ggtgtaaatg gacttgcaga tgcaataagt
     2521 gttggtgcag cagcagctga taacaatatg ccaataatac ttactaatga aaagagtgag
     2581 ttgcaaggtg ctgatgagtt tttaaattca tcaaaaataa ctaagtctta tataattggt
     2641 ggcacagcta ctttatcatc aaatttagaa agtaagcttt caaatccaac aagacttgca
     2701 ggaagtaata gaaatgaaac taatgctaag ataatagata aattctaccc tagttcagat
     2761 ttaaaatatg cttttgttgt taaagatggt tcaaagcgtc aaggagattt gatagatgga
     2821 ctagcagtag gagcattagg tgctaaaact gattcaccag tagttctagt tggaaataag
     2881 ttagatgaaa gtcaaaaaac tgtacttaag tctaagaaaa tagaaactcc tattagagtt
     2941 ggtggaaatg gaaatgaaag tgcttttaat gaactaaata ctcttttagg aaaatagtga
     3001 atagaaagat agtaaggata tacattttat tattaaaaaa tattcaatag aagtttataa
     3061 atataaaata acaaaaaggg gtgtctcaaa atgaactttt tagtccatga ggcactcttt
     3121 tttgtatgaa ttcaaatata ttttcatcat ttcaacaatg gaaaatagga ttgtctatat
     3181 tttgagatag cctctttatt attgatgaaa aatgtaataa ttttaaactg taaaatagtg
     3241 taaagttatt ttataatata aatatagtaa gattttaaac aaaataagga ggaataataa
     3301 tgaaagcacc aaaaactatt ttaacaatac tgacaatagc gcttacttta agtagcattt
     3361 ctataatacc atcatatgca cttacagagg aaaaattagt aggtaatggt agatatgaaa
     3421 ctgctgtaaa gataagtcaa aaggcataca gttctagttc taatgtagtg ttagttaatg
     3481 ataattcatt agctgatgca ctttcagcta caccatttgc aaaggctaaa ggagcaccaa
     3541 tacttttgac agaaagtgat aaactagatg atagaactga aa
//
LOCUS       DAFNNB010000219         3561 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0224, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000219 DAFNNB010000000
VERSION     DAFNNB010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0224"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            122..187
                     /locus_tag="L3M60_003356"
                     /pseudo
     CDS             122..187
                     /locus_tag="L3M60_003356"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(703..1455)
                     /locus_tag="L3M60_003357"
     CDS             complement(703..1455)
                     /locus_tag="L3M60_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HBN6227987.1"
                     /translation="MFAIIFLCIGGFLAAFVDSIAGGGGLISMPVLMAIGVPVHLAIG
                     TNKFAASAGCISSAYRYAKSGKVNNDLLKKLVPFTIVGSILGVRCVLSISEDILNVLV
                     VVMILIVAIYTFTSKNLGQEDNFESINNKNLRLGMLMAFIMGFYDGFFGPGTGTFLTF
                     GFIKIYGYDFLHASANTKILNLTSNITSLLLFMINGQVDYKIAIVFALVMIMGAYIGA
                     KVAIKKGSKMIKPIFLVMALFMVVKLVYQTLV"
     gene            complement(1516..3171)
                     /locus_tag="L3M60_003358"
     CDS             complement(1516..3171)
                     /locus_tag="L3M60_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HBN6227988.1"
                     /translation="MMDFSESNDEVQKFDWGEVTWIHEPSKTQFNRLSAGIVRFFPGN
                     CQEKHFHLSEEQLLYVIQGEGIQIIDGKKISIKEKSIIYCPPYSEHEIINTGKVDLVI
                     LITYVPHKFSSLRQLPIVFSENTIQELINVNIIENLANQISNILKLRISVYDSKHKEI
                     FETKKETKFCDICKSIKECNRKLAKGMNNDNFEKMYHCRYGISSLEIPIVLEENIVGY
                     IECGNFIIYKSIDIDNNLSKLSSSSDVDIKYISKVYNELSLIPKSRLYVLKENLLMMS
                     EFIQEIAKRNFFENQLSIKDEEILKIRKENIRLEEALKKANSKLLEEDYIINPIDMFN
                     NNLKEYPFELELYIEKEIKNMNLEGVYNLIDTNKLIYNDVRDIIKEMIFVLSRTALRD
                     LEDLKLISYLINKYNKKISLVDSDEDLWGVLFEFSNECIDKNRAFWRKDKGNLIENIN
                     KYIQKYYKENISLNSISEVFFISPNYLSSIFNERNKVSITEYINLLRVEESKKYLLDR
                     SFSISDICKKVGFNNSSYFSQIFKKFNNVTPNEYRKNILDNEH"
     gene            complement(3183..3545)
                     /locus_tag="L3M60_003359"
     CDS             complement(3183..3545)
                     /locus_tag="L3M60_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013281026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HBN6227989.1"
                     /translation="MYESRENYLETILILEKKNGIVKSIDIAKQLNYSKASVSRAIGI
                     LKEYGFIIMEVDGDIVLTEEGRKKAEEIYEKHILITKFLVNTLGVSFDIAEKDACRIE
                     HVISKETFEGIKKYLDKN"
BASE COUNT         1253 a          576 c          294 g         1438 t
ORIGIN      
        1 tgatttcaaa ttaaaattag ttgaacctaa tttaaaaggt agttaaattc tatttaaata
       61 gagaagaatg aagcaaaaaa catactgtag aaaaatacat aaaaacttct attagttaga
      121 tacaactata gtagaatata tttactatta taataacaaa agaattaaaa aaattaaatt
      181 agaatagcct tgttaaacgt atatttgaat atgaatataa aattgcataa aattaaaaat
      241 acaggaatga aaattccatt cctgtataaa atctaacttt ttagtctcag tacaatccta
      301 ttttaagata agctcttttg ttttatattt tcttatattt aaaatataac gcatatttta
      361 tgcaacatat tcatattttt ctttatattc tttcatagca tcattccaag cgtcttgtac
      421 tgttacacct cttttattag caattttttg tgccagttca attatcttca tagctcttct
      481 tgatatgttc ataaacattc ctcctacaaa ttaccatata tttcatacac ataaatcaag
      541 attatatttt tatgatatat aatccttcta ttgtaatatt acaatttata tgtgtattat
      601 atatgaacta tattagaaga taattagaat ttttataaaa ctatatgatt aggttaaatc
      661 taaaagattt gcagttactt ataaatttaa aagttctata aactaaacta atgtttgata
      721 taccaatttt acgaccataa acaatgccat tactaaaaat attggcttta tcatctttga
      781 acctttcttt atagcaactt tagcacctat ataagctccc attatcatca ctaatgcaaa
      841 cactatagca atcttataat cgacttgacc atttatcatg aataataata atgaagttat
      901 attacttgtt aagtttaata tttttgtatt tgcagaagca tgtaaaaaat catatccata
      961 tatttttata aatccaaaag tcaaaaaagt tcctgtacct ggaccaaaaa aaccatcata
     1021 aaaacccatt ataaaagcca tgagcatacc taatcttaaa tttttattat taatagattc
     1081 aaaattatct tcttgtccta aatttttaga agtaaaggtg taaatagcta ctattaaaat
     1141 cataactaca accaaaacat ttaatatatc ttcacttatt gataatacac atctcacacc
     1201 taaaatacta ccaacaatag taaatggtac taattttttt aaaaggtcat tattgacttt
     1261 tccagacttt gcatatctat aagcgctact tatacatcct gctgacgctg caaatttatt
     1321 tgtaccaata gcaagatgta ctggtactcc aattgccata agaacaggca tacttataag
     1381 tcctccacct cctgctatag aatctacaaa agctgctaaa aaccctccta tacataaaaa
     1441 aataattgca aacataaaaa tcctccatca ttctctttca atttttctta caatatatat
     1501 gttatatact tttatttaat gttcattatc tagtatattc tttctatatt catttggagt
     1561 tacattatta aacttcttaa atatctgtga aaagtaacta ctattattaa aacctacttt
     1621 cttacaaata tcactaatac taaaacttct atctaacaaa tattttttag attcttctac
     1681 cctaagaaga tttatatact cagttattga gaccttattt ctttcattaa aaatagaact
     1741 caaatagttt ggacttataa aaaagacctc agatatacta tttaaactta tattttcttt
     1801 ataatatttt tgtatatatt tgtttatatt ttcaatcaga tttcccttat cttttctcca
     1861 aaatgctcta ttcttatcaa tacactcatt agaaaattca aataatacac cccataaatc
     1921 ttcatcactg tctacaagtg atattttttt attatattta tttattaaat aagatataag
     1981 ttttaaatct tctaaatctc taagtgctgt tctggataaa acaaatatca tttccttaat
     2041 aatatctcta acatcattat atattaattt atttgtatct attagattat aaactccttc
     2101 taaattcata tttttaattt ctttttcaat ataaagttct aattcaaagg gatattcttt
     2161 taaattatta ttgaacatat ctattggatt aataatgtaa tcctcttcta ataatttact
     2221 atttgctttt ttaagtgctt cttcaagtct tatattttct tttctaatct tcaatatttc
     2281 ctcatctttt atactaagtt gattttcaaa aaaattcctc ttagctattt cctgaataaa
     2341 ttcagacatc attaataaat tctcttttaa cacatataat ctactctttg gaattaatga
     2401 aagttcgtta taaactttac ttatgtattt tatgtcaaca tctgaactgc ttgataattt
     2461 agataaatta ttatctatgt caatagactt gtatattatg aaatttccac attctatata
     2521 tccaactata ttttcttcta atactatggg tatctccaaa ctagaaatcc catacctaca
     2581 atgatacatt ttttcaaaat tgtcattatt cataccttta gcaagttttc tattacactc
     2641 ttttatactt ttacaaatat cacaaaattt agtctctttt ttagtctcaa atatttcttt
     2701 atgttttgag tcatatacag atattctaag cttcaatata ttagatattt gatttgcaag
     2761 attttcaatt atatttacat taattaactc ttgaatagta ttttctgaaa atactatagg
     2821 taactgtctt aaacttgaaa atttatgagg aacataagtg attaatatta ctaaatcaac
     2881 tttaccagta tttataatct catgttccga atatggaggg caataaatta tggatttttc
     2941 ctttatacta atctttttgc catcaatgat ttgtattcct tctccctgaa tcacatataa
     3001 aagctgttct tcacttaaat gaaaatgttt ttcttgacag ttgcctggaa agaaccttac
     3061 aattccagca ctaagtctat taaattgtgt ttttgatggt tcatgtatcc atgtaacctc
     3121 tccccaatca aatttttgta cttcatcatt tgactcacta aaatccatca taaattcacc
     3181 tcttagtttt tatctaaata tttttttatt ccttcaaatg tctctttact tataacatgt
     3241 tctattctac atgcatcttt ttcagctata tcaaatgaaa cacccaatgt atttactaaa
     3301 aatttagtga ttaaaatatg tttttcataa atttcttctg ctttttttct tccttcttcg
     3361 gttaaaacaa tgtcaccatc tacttccatt ataataaaac catattcttt taaaattcct
     3421 attgctctac ttacacttgc cttactataa tttaactgtt tggctatatc aattgatttt
     3481 acaattccat ttttcttttc taaaataagt attgtctcca aataattttc tcttgattca
     3541 tacattttaa catcctccat a
//
LOCUS       DAFNNB010000220         3541 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0225, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000220 DAFNNB010000000
VERSION     DAFNNB010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0225"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            45..1154
                     /locus_tag="L3M60_003360"
     CDS             45..1154
                     /locus_tag="L3M60_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBN6227990.1"
                     /translation="MDFKSLGISENTINILKKSGITTPTPIQKESIKLIKEGKDVIAE
                     AQTGTGKTLAFLLPIFENISLDINDIQVLILSPTRELAIQITEEAMKLKESKDVSILA
                     AYGGKDIGSQIKKLKGNIHMIIATPGRLLDHLNRKTIDLSKLKTFVLDEADQMLLMGF
                     KNEVEAILKETSNKKQTLCFSATINSQVKKLAYRYTKNPVVVSIQKEEITLNNIKQEV
                     VETTDRKKLDALCKVLDEDNPFMAIIFCRTKRRVDNLEEALAIRGYNCQKLHSDIAQS
                     KRERIMKSFRNLDIQYLIATDVASRGLDISGVSHIYNYDLPETPEDYIHRIGRTGRAG
                     EEGYTCAFIDPKNERMLSEIETAIESKINRRIIEL"
     gene            complement(1377..1766)
                     /locus_tag="L3M60_003361"
     CDS             complement(1377..1766)
                     /locus_tag="L3M60_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5071 domain-containing protein"
                     /protein_id="HBN6227991.1"
                     /translation="MENIEELLPKDKFDNSNIEKLKLLSDEEIRPICYRLLEWMQDYN
                     WPVAKEILPVLALHQELITPLIIDVLTEEDDDWKYWIISCLLPLFADKNLEPIVPFVK
                     RIAENPTKWECEAEVDEIAKVFLKRIL"
     gene            complement(1942..3132)
                     /locus_tag="L3M60_003362"
     CDS             complement(1942..3132)
                     /locus_tag="L3M60_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227992.1"
                     /translation="MQKNVKKAKVTGSMLAIFGVASVLFSSHAGGGFATGNQETQYYV
                     QYGWTAPLMAILAMIILTATMREVIIMYNNNDCRNYKDLFCELWRPYPKLEIIWEIYY
                     YLMVLIAVSAVIAGAAAVFQSIGVNYFVAVFIIGIVLLAFTIFGAMLVSKAATAMTIA
                     ILLCTLTIFIVGIKAKAPEITEILSNRTSFTPGYFKPILNTFIYAGFQSVVIPTLAGC
                     SRPLLKNSKEATKAMILSFVMNAIALGLAVTMLMGWYREIIAAGQTTLPTLYVAGQSG
                     NHTIYIIYNVALFLCLMSTGVTTIFGLVNRFEDHKALSFLSSRMKRRVFTACAIMVVS
                     MLISLTGLSNIVKYGYGYCGYLGLFTIVIPFLTLGHYKNKKFAKENPEAKWPAELNEN
                     IENL"
BASE COUNT         1321 a          526 c          446 g         1248 t
ORIGIN      
        1 ttgattaata aaaaagaaaa tttttcatga aaggtaagat aacaatggat ttcaaaagct
       61 tagggattag tgaaaataca ataaatattc taaaaaaatc aggaataaca acacctacac
      121 ctatacaaaa agaaagtatt aaactaataa aagaaggaaa agatgtaata gcagaagctc
      181 aaactggaac aggaaaaacg ctagcttttt tacttccgat atttgaaaat atatcattag
      241 atataaatga tatacaagtt cttatacttt ctcctacaag agaactagct attcaaataa
      301 cagaagaagc tatgaaactt aaagagtcta aagatgtaag tatcctagca gcatatggag
      361 gaaaagatat tggttctcaa ataaagaaac taaagggaaa tattcatatg attatagcta
      421 caccaggtag acttttagac catctaaata gaaaaacaat agaccttagt aagttaaaga
      481 cttttgtttt agatgaggca gaccaaatgt tgcttatggg atttaaaaat gaagtggaag
      541 ccattttaaa agaaacttca aacaaaaagc aaacactatg cttttctgca actataaatt
      601 ctcaagtaaa aaaattagct tataggtaca caaaaaatcc tgttgtggtt tccattcaaa
      661 aagaagaaat aactcttaat aatataaaac aagaagtagt agaaactaca gatagaaaaa
      721 aattagatgc attatgtaag gtgctagatg aagataatcc ttttatggca ataatatttt
      781 gtaggactaa gagaagagtt gataatcttg aagaagctct tgctataaga ggatataatt
      841 gtcaaaaact tcatagtgat attgctcaat caaaacgaga aagaataatg aaatcattta
      901 gaaatttaga tatacaatac ttaatagcta cagatgtagc ttctcgtggt cttgatatat
      961 ctggagtaag tcatatatat aattacgatt taccagaaac accagaagac tacatacacc
     1021 gtattggtag aactggtaga gctggtgaag aaggctatac atgtgctttt attgacccta
     1081 aaaatgaacg aatgttaagt gaaatagaaa cagctataga atctaaaatt aatagaagaa
     1141 ttatagaatt ataatttatg gactaattaa aatgccaata gtatataaat actataatat
     1201 acagaaagca ggtatcaact ttttctttat tataatacta ttttatattg cataaaaaag
     1261 ctgtaacctt tatgattaca gctttttcta tctattgtta gttttatatt acctgtttcc
     1321 aagttgttta atattaaatt ttgaaaataa tctcaataaa tatattcaac tattatttat
     1381 aatattctct ttaaaaatac ttttgctatt tcatctactt ctgcttcaca ttcccattta
     1441 gttggatttt cagcgattct tttaacaaat ggaactatag gttcaaggtt tttatctgca
     1501 aacagtggca aaagacatga aattatccaa tatttccagt catcatcttc ttctgtaaga
     1561 acatctatta ttaatggagt aattaactct tgatgtagag ctaaaacagg taaaatttct
     1621 tttgcaacag gccagttata atcttgcatc cattccaata atctatagca aatcggtctt
     1681 atttcttcat cagataataa ttttaatttt tctatattac tattatcaaa tttgtcttta
     1741 gggagcaatt cttcaatatt ttccataact taattctcct aataaataca ttatgttcat
     1801 tatatctttc aaattttaat ttatcaattt atttttcaac cctattttat catatctaaa
     1861 tagtaatata ctctattttt atttgactat attttcgacc tattaattag attatatctt
     1921 ttatttaaag tactactcac tttataaatt ttcaatattt tcgtttaatt ccgctggcca
     1981 tttagcctct ggattttcct ttgcaaactt cttgttctta taatgtccta atgtaaggaa
     2041 tggaattact atagtaaata aaccaagata accacaatat ccatatccat atttaacaat
     2101 attacttaat cctgtaagtg atataagcat tgacactacc attattgcac aagctgtaaa
     2161 tacccttctt ttcattcttg aagaaaggaa agaaagtgct ttatggtctt caaatctatt
     2221 tacaagacca aatatagtag ttactcctgt agacattaaa caaaggaata atgcaacatt
     2281 ataaattatg tatatagtat gatttccaga ttgacctgca acataaagag taggtagtgt
     2341 tgtttgacca gcagcaataa tttctctata ccatcccatt agcatagtta cagcaagccc
     2401 taatgcaatt gcattcataa caaatgaaag tatcattgcc ttagtagctt cttttgagtt
     2461 cttaagaagt ggtcttgaac atcccgcaag tgtaggtata acaactgatt gaaaacctgc
     2521 ataaataaaa gtattaagta ttggcttgaa atagccaggt gtaaaactcg ttctatttga
     2581 taatatttca gttatttctg gtgctttagc ttttatacca acaataaaaa tagtaagagt
     2641 acataataga atagctatcg tcattgctgt agctgcttta gatacaagca tagcaccaaa
     2701 tatagtaaag gcaagaagta caattccaat aataaataca gctacaaaat aatttacccc
     2761 tatactttga aatactgctg ctgcacctgc tataacagca cttacagcta ttaaaaccat
     2821 tagatagtaa tatatctccc atattatttc aagttttgga tatggtctcc aaagctcaca
     2881 aaataaatcc ttataattcc tacaatcatt attattatac attatgataa cttctctcat
     2941 ggtagcagta agtataatca ttgctaaaat tgccataagt ggtgctgtcc atccatactg
     3001 cacatagtat tgagtttctt gattccctgt agcaaatcct cctcctgcat gtgaactaaa
     3061 aagaacgctt gcaacgccaa aaatggctaa catagagccc gttacttttg ctttctttac
     3121 atttttttgc atgaaatcat cctccctaaa atgatattaa atgtttttat tttaacactt
     3181 cattgataaa acaataacat ttttgttcat taaattcaac tatattttcc caatattttt
     3241 atattttcaa cttttttccc tatctatatt aaatattttt tatatattta ttttattttt
     3301 taagaatgtt caaatctact tcgtttttta attttatgaa atatcttttt taatgttaat
     3361 ttctacagat tttttcttta ttattctata attatttata tataatcata gaataataat
     3421 tatacataat aaaattccct attatatcat attcattgga attatcaaac tttaaaagaa
     3481 taaattaatg taatttgtga aaaaatatac ttcaatataa ataactttga tttatttata
     3541 t
//
LOCUS       DAFNNB010000221         3518 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0226, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000221 DAFNNB010000000
VERSION     DAFNNB010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0226"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            35..1882
                     /locus_tag="L3M60_003363"
     CDS             35..1882
                     /locus_tag="L3M60_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBN6227993.1"
                     /translation="MTKRVLSNFTLTGKNILSKFIFMIRYNFSTLILFELFYKGLALL
                     LILPSIKYIFDNLLKSLNIFYLNMDNIIKILSNPISIVLIILSVIILAFYAFFEFTSV
                     IICFNRSIKCEKIGLFELIKMSFKKSINLLYPKNILLFIFVLLIIPLVNTVLISGFIG
                     KIQIPDYILDYIKSNNILNIVYMISLFIIYILVIRWIFSVHEITLNTENFKIARKESV
                     NLIKGRIIRTVIYSLILSLVMAIIGYVIYHIGIISIGIWTKYYTNIGSLKELFINRCI
                     SFEDYFSLILSIFIFIVDIAFISATYYEYKGVNISRNNIIKEKKSIPKTIFKLSLIIL
                     IFCVESNAYSSHVNGLFNTSFSFNTTTIAHRAGAERAPENTLIALKEALNAKADYAEI
                     DVQETKDGELIILHDSNFKRTTRVDKNVWDVNYDEVRTYDAGSFYYDGKGYYDERFVG
                     EKIPTLDEMIKYSKGRIKLLIEIKLTGHEKGDVTKKVVQLIKENNFENQCIIASMDKT
                     VLQKVKKLDKNLMTCYFMAVAYGDFSKLNYIDVYGIESTFVNTTVVEKIHKMNKKIFV
                     WTVNSYSLIEKMLDLNVDSIITDNPYLVDGAIVWKKNGFINKVSDYLFS"
     gene            complement(3034..3210)
                     /locus_tag="L3M60_003364"
     CDS             complement(3034..3210)
                     /locus_tag="L3M60_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227994.1"
                     /translation="MFIGEIDKCTHILTAYISSSYDYCNFLDTQLDDFISEYGETVVE
                     ICLYQVLLLVSRYN"
BASE COUNT         1414 a          318 c          436 g         1350 t
ORIGIN      
        1 taaaaataca ataaacagat acaaggaggt aaatatgaca aagagagtat taagtaattt
       61 cacactcaca ggaaaaaata tcttaagtaa attcattttt atgataagat ataattttag
      121 tacattaatt ttatttgaat tattttataa aggattggcc ttacttctta tactaccatc
      181 tattaaatat atatttgata atttactaaa aagtttaaat atattttatc taaatatgga
      241 caatattata aaaattttga gtaatccaat atctatagtt ctgattatat tatcagttat
      301 aatattagct ttttatgcct tttttgaatt tacttctgta attatttgct ttaataggtc
      361 aataaaatgt gaaaaaatag gtttatttga acttattaaa atgtcattta agaaatctat
      421 aaatttatta tatcctaaaa atatactatt atttatattt gtgttattaa taattccatt
      481 agtaaataca gttttaattt caggttttat aggaaaaata caaataccag attacatact
      541 agactatata aagtctaata atattttaaa tatagtttat atgatttctc tttttattat
      601 atatatttta gttatcagat ggatttttag tgttcatgaa ataactttga atacagaaaa
      661 ttttaaaata gcaagaaaag aaagtgttaa tcttataaaa ggaaggatta taagaacagt
      721 tatttattca ttaatattat ctttagtaat ggctattatt gggtatgtga tttatcatat
      781 tggaattata tctataggca tatggactaa atactatact aatataggat ctttgaaaga
      841 attgtttata aatagatgta tttcatttga agactatttt agtttgattt tatcaatatt
      901 tatttttata gtggatattg catttatctc agcaacttat tatgaatata aaggagtaaa
      961 tatttctagg aataatatta taaaagagaa aaaatccata ccaaaaacta tttttaaatt
     1021 atcactaata atattaattt tttgtgtaga gtcaaatgca tattcttcac atgtgaatgg
     1081 tctgtttaat acatcatttt cctttaatac aacaactatt gctcatagag caggagctga
     1141 aagggctcca gagaatactt taatagcact aaaagaagct ttaaatgcta aagctgatta
     1201 tgcagaaata gatgttcaag aaactaaaga tggagagctt attatacttc atgattcgaa
     1261 ttttaagagg actacaagag tagacaaaaa tgtttgggat gtaaattatg atgaagtaag
     1321 aacatatgat gcaggaagtt tttattatga tggaaaagga tattatgatg aaagatttgt
     1381 tggagagaaa ataccaaccc ttgatgaaat gataaaatat tctaagggta ggataaaatt
     1441 actaatagaa ataaaactta ctggccatga aaaaggagat gtaacaaaaa aagttgtaca
     1501 attaattaaa gaaaacaatt ttgaaaatca atgtataatt gcatctatgg ataaaactgt
     1561 attacaaaaa gtaaaaaaac tagataaaaa cttgatgaca tgctatttta tggcagttgc
     1621 atatggtgat ttttcaaaat tgaattatat tgatgtatat ggaattgaat caacttttgt
     1681 taatacaaca gtagtagaaa agattcacaa aatgaacaaa aaaatcttcg tttggactgt
     1741 caatagttat agtttaattg agaaaatgct tgacctaaat gtagatagta taattacaga
     1801 taatccatat cttgtagatg gtgcaattgt ttggaagaaa aatggattta ttaataaagt
     1861 atcagattat ttattttctt aatgtttacc atatctaata aaaaagagat attattaaaa
     1921 gtactaatat ctcttttttt attaaaaata aaagcaatta ttaatataag tgtattttat
     1981 tctacgatag tgtgaaagta tacaaacaat aataatatta ttgtattgat ataaataatt
     2041 gtaattgcgt tgttttatct aacaagtgta tattctatat agataggata tttttcaagg
     2101 tttcatatcc atatttatct actaagtctt tatgaaatgt agtttttatt acttctatct
     2161 tacatttttt catgaaagct atctcctcat atatttatat aagtatatgt tctattttaa
     2221 gtttaaacaa gtacctactt ttaagtaaag tactaacatt taggaaagtt atttcgttat
     2281 aatatagaga ttaaataaga atgaaaaatt ataacataga aggttttaat aaatataatt
     2341 tttctcctat ttaaatatgt tgttttgatt ataaacatca ttgagataat acacttaatt
     2401 aataatataa aagaggataa aaattttaaa agtttaattt ttggctaaat taacaatatt
     2461 aatgttagtc tacgattgta aaatatatta taatgtatat ttttataatt actaaataaa
     2521 ataaattaat caatatagtt ttgtaatata aacaaaacat aaaaaatata tataaataaa
     2581 atgattgttt ttcttgcaat atttatataa atattgaaat ttgtctagtt atacgttttt
     2641 attgaccagt tacgcagttg atattgaata ttctaaatat tgatgttatt ttatagatgg
     2701 tttaaacata ctagggggat tttataaatg ttatttgtat gctttataga aaatgtataa
     2761 aatggtaaaa atttagagat atatttgatg aaattacaga atatgtgtat agaatgatgc
     2821 tcatacatta atttagatat aaggaatgta taatttgtat tgaatgattt atttttatgt
     2881 gtatattttt gactgtttta atgaaaaagt aaagatgtac tcccaataca tctttacttt
     2941 agcagaagtt atagttactt ataaagtaca aaaaaattat atatttatta aggtaggtaa
     3001 atctaaaagg gttttcctag gaaaaactta attttaatta tatcgactca ctaataataa
     3061 aacttgatac aagcatattt caactacagt ttctccgtat tctgatataa aatcatctaa
     3121 ctgtgtatct aaaaaattac aataatcata tgaactgctt atataagcag ttaatatatg
     3181 tgtacatttg tctatttctc caataaacat attattcatc cccctaaaaa atttagtaaa
     3241 agtaaaaatt atattttaaa aacaaaataa caaaacttat aaaatttttt agtactagtt
     3301 actataactc tataaaacta aatattccta gttttaccaa tttagtttta ttctacaaat
     3361 atataatact agaagatgct tcattattca acatgcttgt ttgaaaatga aaaatattat
     3421 gaaattatat ttactgtttt gaaaagaaaa actaataata aataatttta tatgtaatta
     3481 tttaaatgaa aaattatgct tttaaattag ctagtttt
//
LOCUS       DAFNNB010000222         3502 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0227, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000222 DAFNNB010000000
VERSION     DAFNNB010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0227"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..879
                     /locus_tag="L3M60_003365"
     CDS             <1..879
                     /locus_tag="L3M60_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siphovirus ReqiPepy6 Gp37-like family protein"
                     /protein_id="HBN6227995.1"
                     /translation="IMHREFMYTETGEKSDVLFVKGVDLKGLLNRRLIVPKTGEAFES
                     HEGTQEEIIKAFVNNNCVTPSSSKRVIDNLIISENKNLGSADMWRSSYENLSDKIQEI
                     TEFCGLGWEITLDAKQKKFIFDVIAGKDLTINQTENPPIIFRSDFDNIRARHFTESII
                     NSKNVVYAGAREDATKLVISVGEVDGFERNEVFADVSENTISILQKEGEIKLKEYEEL
                     KSFELEIDPKNTFIYEKDYNLGDIVTIQDRKLKVTMDTRIVEVQESYSKNGMKLKITF
                     GSSIPTLFSKIKRMVR"
     gene            879..1604
                     /locus_tag="L3M60_003366"
                     /pseudo
     CDS             879..1604
                     /locus_tag="L3M60_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012100884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1578..1820
                     /locus_tag="L3M60_003367"
     CDS             1578..1820
                     /locus_tag="L3M60_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227996.1"
                     /translation="MNKVKEDLASIEITAQRATVEDAENLFEADSQGNKNVEIALNQL
                     AKHYNSLAEQQGNLEIEVNGQRAKGITIVNNLIDMI"
     gene            1837..2982
                     /locus_tag="L3M60_003368"
     CDS             1837..2982
                     /locus_tag="L3M60_003368"
                     /inference="COORDINATES: protein motif:HMM:NF025330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HBN6227997.1"
                     /translation="MTTKLEENAKLREMIDALETAKNDLQVGKNNIANILGLPFTGND
                     KLETTKNTLNSIRSTFVSNLNKKGVSTFANTPFKILAENVGKVEQGNMIVPPWYVPKN
                     VFIEALSTYAKNGIACVSAEKKIYFINGYAATSDSNNLCYDAILNTTTTKNTPSSFSK
                     SALIFYNGYIYKLGGFSGYSAPTTICQKYNILTNSWTEIANMPTARGSACCEMYSNQI
                     HVLYGGEDSSSSQAVRVVEYYNTTLNTWSTKSTIIDSKQSHYFVCAKVSVYKIVVFGL
                     RSSISAHYLTTINYDPVSGTSSKAGEGFDSNTFAVPLGSTLYVSDYGYKAGSGKCGVV
                     IFSDSGIKKLTFDKGTVPSYSYFGASVDGRQIYFILNGKITCFIPEL"
     gene            3001..3366
                     /locus_tag="L3M60_003369"
     CDS             3001..3366
                     /locus_tag="L3M60_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6227998.1"
                     /translation="MIKIWDKKEKINGVEAEEILKSNYDFQTSEVFLILDDYERVTNI
                     ESVNTIKSIYKLDKSLTALETAEKYLEMHKKQEEEIKAHEELEKNSKNATIATHEEVK
                     ALREETAALTFAMIEKELI"
     gene            3367..3501
                     /locus_tag="L3M60_003370"
     CDS             3367..3501
                     /locus_tag="L3M60_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HBN6227999.1"
                     /translation="MWYKIIKKYYEDELWSKEQVKTAVEKNKITSTEYKEIVGEDYIV
                     "
BASE COUNT         1429 a          407 c          666 g         1000 t
ORIGIN      
        1 ataatgcaca gagaatttat gtatacagaa acaggagaaa aaagcgacgt tctatttgtc
       61 aaaggtgtag atttaaaagg tttattaaat agaaggctca ttgtaccaaa gactggagaa
      121 gctttcgaaa gtcacgaagg gacacaagaa gagattataa aagcttttgt taataataat
      181 tgtgtaaccc cttcaagctc aaaacgtgta attgataatc ttatcatttc agaaaataaa
      241 aatcttggaa gtgctgatat gtggcgcagc tcttatgaaa atttaagcga taaaattcaa
      301 gaaataacag aattttgtgg gcttggttgg gagataacat tagatgctaa acaaaagaaa
      361 tttatttttg acgtaatcgc gggtaaagat ttgactatta atcaaacaga aaatccaccg
      421 attatttttc gaagtgattt cgataacata agagctagac actttacaga gagtataatt
      481 aattctaaaa atgttgtgta tgctggagca agagaggatg caacaaagct agtaatttca
      541 gttggtgagg ttgatggttt tgagagaaac gaagtctttg cagatgtaag cgaaaatact
      601 ataagtatac tacaaaaaga aggcgaaatt aaattaaaag aatatgaaga gttaaaaagt
      661 tttgaattag aaatagaccc taaaaatact tttatatatg aaaaagatta caatctaggt
      721 gatattgtta caattcaaga tagaaaattg aaagtaacaa tggatactag aattgtagaa
      781 gtacaagaaa gctacagtaa aaatggcatg aaattaaaaa ttacgtttgg gagcagtata
      841 ccaaccttat tttcgaagat aaaaaggatg gtgagatgat ggaaaaaagt tttgtattta
      901 atagcataaa tggcgatagg agatacaagg cagaggactt tagagaatac tttgcaagct
      961 tcataggaaa tggtgtattc cctaatccaa gtacaaattt gcaagtcgtt gataataata
     1021 atatgactat aacagtaaaa aaaggtaagg gctggattaa cggagctatc tatataaaca
     1081 cagacgattt agtaattaat atagacgcag cggacgggat tttaaataga atagacagag
     1141 tagtcttaag atttgatact ttaaatagaa atattaaatt agctgttaaa aaaggtactt
     1201 ttaatagttc cccagctgca acagaattac aacgtgatgc agatgcgtac gaacttggat
     1261 tagctgatat ttacatcaaa gctggagcaa taagtattac acaaagttct ataacagatt
     1321 taagattaga taaaaatctg tgcggaattg taaaaggtac agtagaagaa atagacacaa
     1381 caacattact tgcgcaattg aacgcttgga aggatgaaga aataagtaat ttcaatgctt
     1441 ggcgggaaaa tcaagagaat aaacaagaag attggtataa cactacaaca acaaactttg
     1501 tatcagaatt taacacatgg tttgaaagta taaaaggcat tttggatgag gatgcagctg
     1561 gaaacttatt aaatgaaata aacaaagtaa aagaagattt ggcaagtatt gaaataacag
     1621 cacagcgagc tactgtagaa gatgcagaaa atttatttga agctgattcg caaggaaata
     1681 aaaatgttga aatagcatta aaccaattag cgaaacatta taactcttta gcagagcaac
     1741 aaggtaattt agagatagaa gtaaatggac aaagagcgaa aggaattact atagtaaaca
     1801 acttaataga tatgatttaa taaaggagcg tgaaagatga caacgaaatt agaagaaaat
     1861 gcaaaactaa gagaaatgat agatgcatta gaaactgcaa aaaatgactt gcaagtaggt
     1921 aaaaataata tagctaatat tctgggctta ccttttacag gaaatgacaa gttagaaaca
     1981 actaagaata cattaaattc aattagaagt acttttgttt caaatttaaa taaaaaaggt
     2041 gtgtcaacat ttgctaatac accttttaaa attttagctg aaaatgtagg gaaggtagaa
     2101 caaggcaaca tgattgttcc accttggtat gttcctaaaa atgtatttat tgaagctttg
     2161 agtacttacg ctaaaaatgg aatagcatgt gtttcagcag aaaaaaaaat ctactttata
     2221 aacggttatg cagctacgtc ggattcaaat aacctttgct atgatgctat tttaaacaca
     2281 acaacaacta aaaatacacc tagttctttt tcgaagtctg cattaatttt ttataatgga
     2341 tatatctata aattaggtgg ttttagtggc tattcagctc ctacaactat atgtcaaaaa
     2401 tataatattt taactaattc atggactgaa atagcaaata tgcctaccgc aagaggaagt
     2461 gcttgttgtg aaatgtattc aaatcaaata catgttttgt atggcggaga agattcttct
     2521 tcatctcaag cagttagagt tgttgagtat tataatacaa cgttaaatac ttggtctaca
     2581 aaatcaacta taatagattc aaaacaatcg cattattttg tttgtgctaa agtcagcgtt
     2641 tataaaattg ttgtgtttgg gctaagaagt tctatttctg ctcactatct tacaacaatc
     2701 aattatgacc ctgtaagcgg aactagcagt aaggcaggag aaggctttga tagtaatact
     2761 tttgccgtac cactaggttc aactttgtat gtgtcagatt atggttataa agctggaagt
     2821 ggcaaatgtg gagttgttat tttttctgat agtggaatta aaaaattaac ttttgataaa
     2881 ggaactgttc ccagttatag ctattttgga gcttcggttg atggtagaca aatatatttt
     2941 attttaaatg gtaagattac ttgttttata ccagaattat aaaaaaagga gagaatgaaa
     3001 atgataaaaa tatgggataa aaaagaaaaa ataaatggag tagaagcaga agaaatttta
     3061 aaaagtaatt atgattttca aactagcgaa gtgtttttaa tacttgatga ttatgaaaga
     3121 gttacaaata tagagtcagt taatacaatt aaatctatat ataaactaga taagagtttg
     3181 acagctttag aaacagcaga aaaatattta gaaatgcata agaaacaaga agaagagata
     3241 aaagcacatg aggaactaga aaaaaattct aaaaatgcaa ctatagctac acacgaagaa
     3301 gtaaaagcat taagagaaga aacagcagca ttaacttttg caatgattga aaaggagttg
     3361 atatagatgt ggtacaaaat aataaaaaaa tactatgaag atgaattgtg gtctaaagag
     3421 caagttaaaa cagcagtaga aaagaataag ataacatcaa cagaatataa agaaattgta
     3481 ggagaggatt atattgtata gt
//
LOCUS       DAFNNB010000223         3452 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0228, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000223 DAFNNB010000000
VERSION     DAFNNB010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0228"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..917
                     /locus_tag="L3M60_003371"
     CDS             <1..917
                     /locus_tag="L3M60_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBN6228000.1"
                     /translation="FNYYIWVVIVFRMLLFLFNYTVIYEVKEPKENIIGNNLIRIDIS
                     IDNNFMLYLAYLWLVVTIVIAVYTFVKYTRFKNLVVDVSYDIEDNDVNCIYKNLLKKL
                     NIKKKIELRSSDELISPAGMGLFKSYIFLPDYPYSKDELNWILKHELMHFKNKDILIK
                     FLVFSVKTIYWFNPLVYIMSNKVNLDCELCCDESVLMDCSLEDKKEYALALIKSIKLS
                     KNYNSGILTTEFNKTSLEKRLDSIVKKKGKSGILVLLISFALFSVTYFNFESISFDNS
                     NSLRNIGILEKTGADLKEEDDISVRVVN"
     gene            979..1581
                     /locus_tag="L3M60_003372"
     CDS             979..1581
                     /locus_tag="L3M60_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBN6228001.1"
                     /translation="MKSLKKILIVTGCLTIISTSLAFASTNNDVNVSKSNEDQATNSF
                     TITGYEFTYENAPQPIKSDYEKKCKELNINPSKNDTIFINEKFIDSYNINIDDYNIEP
                     KAFIIRYKKDLNRFDVTGPISYSVNINTTVVGYGHIEKGNPVHLVQLLCQQINGSSIN
                     PDSKFGPDTYNQVKILQGRLGVTKDGVVGKATWQAAGERY"
     gene            complement(1715..2731)
                     /locus_tag="L3M60_003373"
     CDS             complement(1715..2731)
                     /locus_tag="L3M60_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBN6228002.1"
                     /translation="MNYNDLLKSARENFNEKCKVCKICNGLACAGNVPGMGGKGSGSS
                     FIENRKSLEKIKINMRVIHNVSKPDTSIELFGRKMSSPIFAAPVSGTLLNMGGKVSEK
                     EYIEPVVRGCSNSGIYAMVGDTNVDTFLLDNLDVLKDNCGNGIVFIKPWNNSKIIEKI
                     RLSEEAGAFAVGVDLDACGLINNQLQENPFSPKTIDEIRELVESTRLPFIIKGIMTVD
                     DALMTVESGASAIIVSNHGGRVLDYTPGTCEVLPDIAKAVKGKITILVDGGVRSGVDV
                     VKMLGLGADAVLMGRPFVIASFGGGLDGVEFFIEKVRSELCETMILTACQNVKDIDGR
                     VIWK"
     gene            complement(3061..3243)
                     /locus_tag="L3M60_003374"
     CDS             complement(3061..3243)
                     /locus_tag="L3M60_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228003.1"
                     /translation="MYKDESDECIHLMTAYIDSLSGYYSFIDTQLEDFMVKYGENIVD
                     SNLHSIMMLLCKWGLS"
BASE COUNT         1318 a          478 c          462 g         1194 t
ORIGIN      
        1 gattcaatta ttatatttgg gtagtaatag tctttagaat gttacttttt ctatttaatt
       61 atacagtaat atatgaagta aaagagccca aagaaaatat aataggaaat aatcttatac
      121 gaattgacat ttctatagat aataatttta tgctgtattt agcttattta tggttagttg
      181 taaccatagt cattgcagtt tatacatttg taaagtatac aagatttaaa aatcttgttg
      241 ttgatgtatc ttatgatatt gaagataatg atgtaaactg tatctataaa aatttgttga
      301 aaaaattaaa tataaaaaag aaaattgagt taagaagttc tgatgaattg ataagtccag
      361 cggggatggg gttatttaaa tcgtacatat ttttgccaga ttatccttat agtaaagatg
      421 agctaaattg gattttgaaa catgaattaa tgcattttaa aaacaaagat attttaataa
      481 agtttttagt tttttctgtt aaaacaatat attggttcaa tccacttgtt tatataatga
      541 gtaataaagt aaatctagat tgtgaattat gttgtgatga aagtgttttg atggattgtt
      601 ctttagaaga taagaaagaa tatgcattag cacttataaa gtcaattaaa cttagtaaga
      661 attataatag tggtattttg acgactgaat ttaataaaac aagtttggaa aagagacttg
      721 acagtattgt taagaaaaaa ggtaaaagtg gaatattggt gttattgatt tcgtttgcat
      781 tattttcagt tacatatttt aattttgaaa gtattagttt tgacaattca aattctttaa
      841 ggaatatagg tattttagaa aaaacaggtg cagatcttaa ggaagaagat gatattagtg
      901 tgagagttgt aaattaacat atttttgcag tgttaaaaga acattataat ttattaacaa
      961 attaggagga attttgaaat gaaaagtcta aagaaaatct taatagtcac agggtgcttg
     1021 actataatat ctactagttt agcatttgca agtactaata atgatgtaaa tgtatcaaaa
     1081 agtaatgaag atcaagcaac aaattcgttt acaataactg gttatgaatt tacttatgaa
     1141 aatgcaccac aacctataaa atctgattat gaaaaaaaat gtaaagaatt gaatataaat
     1201 ccatctaaaa acgatacaat ctttattaat gaaaaattta tagattcata taatattaat
     1261 atagatgatt ataatattga accaaaagca tttattatta gatataaaaa agacttaaat
     1321 cgttttgatg ttactggacc tataagttat agcgtaaata taaatactac tgtagtaggg
     1381 tatgggcata tagaaaaagg taatcctgtg catttagtac aacttttatg tcaacaaata
     1441 aatggttcta gtattaatcc agatagtaaa tttgggcctg atacatataa tcaagtaaaa
     1501 atacttcaag gaagattagg tgtaacaaaa gatggtgttg ttggaaaagc tacttggcaa
     1561 gctgctggag agcgttatta gaaaagaggt tgtcgcacta agaaataggg cggcaacctt
     1621 tttatataga gtatattaat cagtatcttc taaaaatagt tgttattata tctaaagagt
     1681 ctggaaatat aagtgttata aaactatttc acaatcactt ccatataact ctaccatcta
     1741 tatccttaac attttgacaa gcagtcaaaa tcatagtctc acacaattca cttctaacct
     1801 tctctataaa aaactccaca ccatcaagac caccaccaaa agaagcaata acaaagggtc
     1861 ttcccatcaa aacagcatca gcacctaaac caagcatctt aacgacatca acaccacttc
     1921 taacaccacc atcaacaagt attgttattt ttccctttac agctttagct atatctggta
     1981 gtacctcaca agtaccaggg gtataatcaa gtactcttcc gccatgattt gaaacaataa
     2041 tagcactagc accagattct acagtcatca aagcatcatc tacagtcata attcccttta
     2101 taataaaagg caatctagta gactcaacca attctctaat ctcatcaatt gtcttaggag
     2161 aaaaaggatt ttcttgaagc tgattattga taagtccaca agcatcaagg tctacaccaa
     2221 ctgcaaaagc acctgcttct tcagacaatc taatcttttc tattatcttt gaattattcc
     2281 aaggctttat aaatacaatt ccatttccac aattatcttt taacacatct aaattatcca
     2341 gtaaaaaagt atctacattg gtatcgccaa ccatagcata aattccagaa ttagaacaac
     2401 ctctaacaac aggttctatg tattcttttt cagaaacttt accacccatg ttcaataaag
     2461 tacctgatac aggggcagcg aatataggag aactcatttt tctaccgaat aattctatag
     2521 atgtatctgg ctttgaaaca ttatgtatta ctctcatatt tatctttatt ttttctagac
     2581 tctttctatt ttcaatgaaa gatgaaccac ttcctttacc acccatacca ggaacatttc
     2641 cagcacaagc caatccatta caaattttac atactttaca tttctcatta aagttctctc
     2701 tagctgattt tagtaagtca ttataattca tacaaaatct ctccttttta taatattaaa
     2761 attaagcatt actatattga aaatatctta gttgtaaata tagagatgat gttatgatga
     2821 gataaactag ataatacata ttagatattt tttattgttg cagatagtta aattatatca
     2881 tattagatag taaaattttg aaaaaaacta acttatttaa gtatacattt taaaaaaatt
     2941 aaaaaagcta tatatagatt tttaaagaaa tctatatata gcttttaagt ttatgacaat
     3001 ctttttttat taatgaataa ataatataga atacaatgta caaaaaagct tcttaaaatg
     3061 ctaagataat ccccatttac aaagcaacat cattatacta tgcaagtttg aatcaacaat
     3121 gttttcacca tatttcacca taaaatcctc taattgtgtg tcaataaatg aataatatcc
     3181 acttagacta tctatgtatg cagtcattaa gtgtatacat tcatcactct catctttata
     3241 catatatatc tccacctata tacaatatat aaatttagat actaaatcgt atcatattaa
     3301 atagttaagt atgtcacatc tatgtagtta tatatttata tccttatatt aataactaaa
     3361 gtgaattttg acaatagatt ttgggaaact ggatgttcaa aatgtaaact tggattttaa
     3421 aatgattaat taaaatagtg catagtatat at
//
LOCUS       DAFNNB010000224         3405 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0229, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000224 DAFNNB010000000
VERSION     DAFNNB010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0229"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            16..1005
                     /locus_tag="L3M60_003375"
     CDS             16..1005
                     /locus_tag="L3M60_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBN6228004.1"
                     /translation="MELKDRSLVNNQYKGKIKSANLSVDYEISQVMKDIDKLGLNTVN
                     VPIIINVESINSDIMSIDNNSKEKAIKLIKKLNKKGISTILEAYPWIENGKLYETDWN
                     PINKKRFFYTWQNTILNEIIIDVANPLDVNVLNIGSNFVHLEGYQQNWSEIIDFVQSK
                     FDGLVTYRTNWWYTKKDDLNSKLSYEQKLNNNFFDKLDFISIAAYFELSNKRINTVDE
                     LISALYSSTVNNRRQNIKKEIYNLYKAHRKPIFFGELGFSNREYASSQPWNHATSKVV
                     NGEEQARCFEAYKKVFENEEWINGFSVFCIGKIDDEKNFYPSKESIKVIKSWY"
     gene            1062..2186
                     /gene="wecB"
                     /locus_tag="L3M60_003376"
     CDS             1062..2186
                     /gene="wecB"
                     /locus_tag="L3M60_003376"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBN6228005.1"
                     /translation="MNDIKVMTVFGTRPEAIKVAPLIKELEKRANIKSIVCVTAQHRE
                     MLDQVIETFNINIDYDLDIMEKGQSLNDITCKILNKLPLILNKENPNIVLVHGDTTTT
                     LATSLTAFYNKIIVGHIEAGLRTYDKYSPFPEELNRQLTGVIADMHFAPTNLAKKNLL
                     SEGKPYNNIFVTGNTAIDALKMTIKENYSHPIIDDIGNDRMILLTSHRRENLGKPMKN
                     IFKAIKRIVDDFEDVQVVYPIHLNPKIRTIADEIFGKFPKKIHIIEPLDVADFHNFLN
                     KSYMIMTDSGGIQEEAPSLGKPVLVLREKTERTEGIEANTLKLVGTNEDRIYNSVSNL
                     LINKDNYVQMSKASNPYGDGNASKYIVDIIIKKFNCKYLN"
     gene            2294..3268
                     /locus_tag="L3M60_003377"
     CDS             2294..3268
                     /locus_tag="L3M60_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HBN6228006.1"
                     /translation="MARKLIKNLGKSKSAKRVKLLFKKIFITVFIVASIVAIFNITKY
                     FEELYKVRDLKSTKIEYYMDVADEAGDGKVQLSWKALLAIDMVIHDEDLSNIKKKDTL
                     DIGEKFIVEDKNDKGEKVYKVKKFNKVLSELKFDSSQKSRARKYMKDLEYTYLGNKQL
                     DSSDEKIKFIKKLEDSAIREYIDYGILPSITIGQAILESGWGNSKLTKQSNNLFGIKA
                     DKAWKGKSVEISTSEHYNEKIVASFRSYNSLQDSVKDHSLFLINNKRYRKHGLFEAKD
                     YISQAQALENAGYSTAEDKKGNRIYAELLVDVIRSYNLQLIDNKVETK"
BASE COUNT         1420 a          334 c          596 g         1055 t
ORIGIN      
        1 atttactttg taagtattga gttaaaagat aggagtctag taaataatca atataaaggt
       61 aagattaaat cagccaattt atctgtagat tatgaaataa gtcaagttat gaaggatata
      121 gataaattag gattgaatac agtaaatgta cctataatta taaatgtaga gtccattaat
      181 tctgatatta tgtcaataga taataatagt aaagaaaagg caattaagct aataaaaaaa
      241 ttaaataaaa aaggtatttc aacaatatta gaggcatatc catggattga aaatggaaaa
      301 ttgtatgaaa ctgactggaa ccctataaat aaaaaaagat ttttttatac ttggcaaaat
      361 actatattaa atgaaattat aattgatgta gcaaatccac tggacgtaaa tgttttaaat
      421 attggttcta attttgtaca tttggaaggg tatcaacaaa attggagcga aataatagat
      481 tttgtacaat ccaaatttga tggtcttgta acgtacagaa ctaattggtg gtatacaaaa
      541 aaagatgatt taaatagtaa gctgtcttat gagcaaaaac tgaataataa tttttttgat
      601 aagttagatt ttatatctat agcagcatat tttgagttaa gcaataaacg tattaataca
      661 gtggatgaat taatttctgc cttgtactca tctactgtaa acaatagaag acaaaatata
      721 aaaaaagaaa tatataacct ttataaggct catagaaaac ccatattttt tggagaatta
      781 ggtttttcaa atagagaata tgcaagttct caaccgtgga atcatgctac ttcaaaagtt
      841 gtaaatggcg aagaacaagc aaggtgtttt gaggcatata agaaagtttt tgaaaatgaa
      901 gaatggataa atggtttttc tgtgttttgt ataggtaaaa tagatgacga aaaaaatttt
      961 tacccatcaa aagagagtat aaaagttatt aagtcttggt actaataatt ttgaaagttt
     1021 agaatttata gatgttaaat agatgattag gagagatata aatgaatgac atcaaggtta
     1081 tgactgtatt tggaactaga cctgaagcta tcaaagtagc acctttaata aaggaacttg
     1141 aaaaaagagc aaatataaaa agtattgtgt gtgttacagc tcaacataga gaaatgcttg
     1201 accaagttat agaaaccttt aatataaata tagattatga tttggatata atggaaaaag
     1261 gacaaagtct gaatgatata acatgtaaga ttttaaataa acttcccttg attttaaaca
     1321 aagaaaatcc aaatatagta ttagtgcatg gagacacaac cacaactctg gctacaagct
     1381 tgactgcatt ttataataaa attattgtcg gtcatataga agctggtctt agaacatatg
     1441 ataaatattc tccatttcca gaagagttaa ataggcaact tacaggtgtt attgctgata
     1501 tgcactttgc tcctactaat ttggctaaaa aaaacttgct ttctgaagga aagccctata
     1561 ataatatatt tgtaactggt aatactgcta ttgatgcttt aaagatgaca ataaaagaaa
     1621 attatagtca ccctataatt gatgatattg gcaatgatag gatgattttg ttaacctctc
     1681 acagaagaga aaatttgggt aagcctatga aaaatatatt caaagctata aagcgaatag
     1741 tagatgattt tgaagatgta caggtagtat atcccatcca tttaaatcct aaaatacgaa
     1801 ctatagctga tgagatattt gggaaatttc ctaaaaaaat tcatattatt gaacctttag
     1861 atgtagctga ctttcataac tttttaaata aatcttatat gataatgact gatagtggag
     1921 gaatacaaga agaagctccg tcattaggta agccagttct tgtgttgcgt gaaaaaacag
     1981 aaagaacaga aggaattgaa gcaaatacac ttaaattagt aggtactaat gaagatagaa
     2041 tatataattc agtgagtaat ttattaataa ataaagataa ctatgttcag atgagtaaag
     2101 catccaatcc atatggggat ggaaatgcaa gcaaatatat agttgacata ataattaaaa
     2161 aatttaattg taagtattta aattaggttt ttatgctcaa cttgaaattt atattagata
     2221 agaattaaaa cattaacgaa ttgatataag atttattata ctatgaggga tacaattttt
     2281 aagggggaat aatatggcta gaaaattaat aaaaaattta ggtaaaagta aaagtgcaaa
     2341 gagagtgaag cttttattta agaagatttt tattactgta ttcatagtag caagtatagt
     2401 tgctattttt aatataacga aatattttga ggaattatac aaagtaagag atttaaaaag
     2461 tactaaaatt gaatactata tggatgtagc tgatgaagca ggggatggaa aggttcagtt
     2521 aagttggaag gccttacttg ctatagacat ggtaatacat gatgaagatt taagtaatat
     2581 aaaaaagaaa gacacattgg atataggaga aaaatttata gtagaagata aaaatgataa
     2641 aggcgaaaag gtgtataagg taaaaaagtt taataaggta ttaagcgaat tgaaatttga
     2701 ctcttctcaa aaaagtagag caagaaaata catgaaggat ttggaataca cataccttgg
     2761 aaataaacaa ttagatagta gtgatgaaaa aattaaattt ataaagaaat tagaagattc
     2821 agctataaga gaatatattg attatggaat attgccttct ataacaattg gacaagctat
     2881 attagaatct ggctggggaa attctaaact tacaaaacag agtaataatt tatttggtat
     2941 aaaagcagat aaagcatgga aaggaaaaag tgtagaaatt tcaacttcag agcattataa
     3001 tgaaaaaatt gtagctagtt ttaggtctta taattcatta caagattctg tcaaagacca
     3061 cagtttattt ttaattaata ataaaagata tagaaaacat ggattgtttg aggcaaaaga
     3121 ttatattagt caagctcaag cattagaaaa tgcagggtat agtacagcag aagataaaaa
     3181 aggaaatcgt atatatgcag agctattagt agatgttatt aggagttata atttgcaact
     3241 gatagacaac aaagttgaga caaaataatt gttaactcta tatgaagaga tggcaagatg
     3301 agattatttt ctacaaaaga atagaaaaat tgtaaaaaaa actaaaaaaa tgtattgact
     3361 tgttatttaa aaggtgatat tattattact gtactcgaaa gagaa
//
LOCUS       DAFNNB010000225         3372 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0231, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000225 DAFNNB010000000
VERSION     DAFNNB010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0231"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..2599
                     /locus_tag="L3M60_003378"
     rRNA            <1..2599
                     /locus_tag="L3M60_003378"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2887..3001
                     /gene="rrf"
                     /locus_tag="L3M60_003379"
     rRNA            2887..3001
                     /gene="rrf"
                     /locus_tag="L3M60_003379"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            3158..3234
                     /locus_tag="L3M60_003380"
     tRNA            3158..3234
                     /locus_tag="L3M60_003380"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3192..3194,aa:Met,seq:cat)
BASE COUNT          866 a          821 c          987 g          698 t
ORIGIN      
        1 ggggttagaa gcgaacccgg ggaactgaaa catctaagta cccggaggaa aggacatcaa
       61 acgagactcc gtaagtagtg gcgagcgaac gcggaccagg ccagtggctt ttatgagaca
      121 agtggaacac cttggaaagg gtgaccgaag tgggtgatag tcccgtacac gtaatgcgaa
      181 taaaagtcct tgagtagggc gggacacgtg aaatcctgtc tgaacatggg gagaccactc
      241 tccaagccta agtactcgtg catgaccgat agcgaaccag taccgtgagg gaaaggtgaa
      301 aagcaccccg acaaggggag tgaaagagat cctgaaaccg aatgcctaca aacagttgga
      361 gcccaagatt tgttctgggt gacagcgtac cttttgtata atgggtcagc gacttagtct
      421 gacgagcaag cttaaaccgg taggtgtagg cgcagcgaaa gcgagtctga acagggcgtt
      481 cagttcgtcg gattagaccc gaaaccaagt gatctagcca tgagcaggtt gaaggtaagg
      541 taacacttac tggaggaccg aacccgcatc tgttgcaata gattgggatg acttgtggct
      601 aggggtgaaa ggccaatcaa acttggagat agctggttct ccgcgaaatc tatttaggta
      661 gagcgtcgat cgaatacctt cgggggtaga gcactggatg ggctagggga tctcaccgat
      721 ttaccaaacc taaccaaact ccgaataccg aagagtacta atcggcagac acacggcggg
      781 tgctaacgtc cgtcgtgaag agggcaacaa ccctgaccac catctaaggt ccctaagtta
      841 tggctaagtg ggaaaggatg tgaggatccc aaaacaacca ggatgttggc ttagaagcag
      901 ccatcattta aagaaagcgt aacagctcac tggtctaaat aagggtcttt gcgccgaaaa
      961 tgtaccgggg ctaaagccat acaccgaagc tgtggattct ttggaacctt cgggttcccg
     1021 ggagtggtag cggagcgttc tgtaagccgt tgaagagaga cccgtgaggg ctcttggagg
     1081 tatcagaagt gagaatgctg acatgagtaa cgataaaggg agtgagagac tccctcgccg
     1141 aaagtccaag ggttcctgct taaagttaat ctgagcaggg ttagccggcc cctaaggcga
     1201 ggccgaaagg cgtagtcgat gggaaccacg ttaatattcg tgggcctgcg ggtagtgacg
     1261 gatcgcgtat gttgttcttc cttattggat tggaagggct tcgaagcggt tccaggaaat
     1321 agctcccgcg tatagaccgt accctaaacc gacactggtg gactggtaga gaataccaag
     1381 gcgcttgaga gaactgcgtt gaaggaactc ggcaaattgc acgcgtaact tcggaagaag
     1441 cgtgaccctt atgtacgcaa gtatgtgagg gtggcacaga ccagggggta gcgactgttt
     1501 accaaaaaca cagggctctg cgaagtcgca agacgacgta tagggtctga cgcctgcccg
     1561 gtgctggaag gttaagagga gaggtgcaag ctttgaatcg aagccccagt aaacggcggc
     1621 cgtaactata acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacctgcacg
     1681 aatggcgtaa cgacttcccc gctgtctcca acgcagactc agtgaaattg aattccccgt
     1741 gaagatgcgg ggttcctgcg gttagacgga aagaccccgt gcacctttac tatagcttta
     1801 cactggcatt cgtgtcggca tgtgtaggat aggtggtaga ctttgaagcg ggggcgccag
     1861 ccttcgtgga gtcatccttg aaataccacc cttacctata tggatgtcta accgcggtcc
     1921 gttatccgga tccgggacag tgtatggtgg gtagtttgac tggggcggtc gcctcctaaa
     1981 gagtaacgga ggcgcgcgat ggtgggctca gaccggtcgg aaatcggtcg tcgagtgcaa
     2041 tggcataagc ccgcctgact gcgagactga caagtcgagc agagacgaaa gtcggtcata
     2101 gtgatccggt ggtcccgcgt ggaagggcca tcgctcaacg gataaaaggt acgccgggga
     2161 taacaggctg atgaccccca agagtccata tcgacggggt tgtttggcac ctcgatgtcg
     2221 actcatcgca tcctggggct ggagcaggtc ccaagggtat ggctgttcgc catttaaagc
     2281 ggtacgtgag ttgggttcag aacgtcgtga gacagttcgg tccctatctg ccgtgggtgt
     2341 aggaatattg acaggatctg tccctagtac gagaggaccg ggatggacgt atctctggtg
     2401 gacctgttgt ggcgccagcc gcatagcagg gtagctatat acggacggga taaccgctga
     2461 aggcatctaa gcgggaaacc cacctgaaaa cgagtattcc cttgagagcc gtggaagacc
     2521 accacgttga taggccgggt gtggaagtgc ggcaacgcat gaagcttacc ggtactaata
     2581 gctcgattgg cttgatcatt ctcattactt atatccatcg cgaagcgatg aattgactgt
     2641 ctcacgcgcc gtatcgcccc tttggggcgg gcgctgcgac ggcgcgccgg acgtccggcg
     2701 acgacctagc ggtctgtata ggtactttct ggaacttcgg ttctaggaaa agctccaaat
     2761 acagcacgat agtgcgtcgc ggctcgtgcc gcgcccccgc ggagcacagc ccgatagggc
     2821 tgatatgcgt gagcgacatt caacacaaac aaaaccagct tctctttcat cgttgctttt
     2881 tgctgacctg gtggttacag cggagcggct gcacccgatc ccattccgaa ctcggccgtg
     2941 aaacgctcca gcgccaatgg tacttcgtct caagacgcgg gagagtaggt cgctgccagg
     3001 tctgccaaac gcaacatgaa atcataatct ctccacatcc catacgttcc aagcaacagg
     3061 ccgcatcaag cggccttttg cttttgcaaa acagcaggca caagtgcata attacacgcc
     3121 gcttcaaacg cggtagccgg aaataccggc atggtggcgc ggggtggagc agcccggtag
     3181 ctcgtcaggc tcataacctg aaggtcacag gttcaaatcc tgtccccgca accaatctcc
     3241 caaaaacatc tcaaaacaag cttctcgcaa aacacaaaca ttaaccccgc tcccgcgggg
     3301 ctttgtcgtt tgaggaaggc acaaaatacc agcgcccgaa gttccttgtg cagcaatata
     3361 ttcgtcggct ta
//
LOCUS       DAFNNB010000226         3346 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0232, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000226 DAFNNB010000000
VERSION     DAFNNB010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0232"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..615
                     /locus_tag="L3M60_003381"
     CDS             <1..615
                     /locus_tag="L3M60_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228007.1"
                     /translation="YQQNINEIKFSIGSKLYLEIVNNQSTNEDIEEHDNYSNFIIYDI
                     EENTFNISDKFMDNLNKEKNANPKEIGLLGKTKLDYMVKNNKLNLIVTDMSNSITNLV
                     YDINSNKIKLEDYEILDLKVDYNEASSGIRKVKLLDDKIYSISGIKNNLSGELKGFKG
                     VVDIVGTNPVKFKVFDMKLKKTVYEAELINGDVSLQEKLFFVAN"
     gene            972..2153
                     /locus_tag="L3M60_003382"
     CDS             972..2153
                     /locus_tag="L3M60_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBN6228008.1"
                     /translation="MNKKYIKAGNLIDGTGNSTMQNKGIIIDGDTIVEIEDIREDLNG
                     YDVYDYLDKTVMPGIMNCHVHLTMEPVGNPKVYYDNVSDVELVVNTIKQLDAYLDSGV
                     TYIRSLGCPKYVDVQLKNLIDKGSIDGPGIVTSGPVICMTGGHGYYFGIESDGVDECR
                     KSARTVLKNGVDCVKIMATGGVTTDGVEPGSPQLTLEEMKAAVDEAIKAGKITATHAQ
                     GRTGIKNAVLAGITSIEHGVYLDDEIIDLMLERGTYLVPTVAAPYFILNAGKESGITE
                     ATLRKCEIVAIPHKESFLKAYKKGIKIAVGSDAGTSYNEHGKTYYEMKLMSDYGMDNM
                     DIIVAATKTASELLRIDKNYGTLEKGKKADVIVVDGNPIENIDVLADVLAVFKLGKKV
                     R"
     gene            complement(2295..3212)
                     /locus_tag="L3M60_003383"
     CDS             complement(2295..3212)
                     /locus_tag="L3M60_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228009.1"
                     /translation="MENNKCREDFRFTQEYEEDYMNANDRYYENYQVADRYYNYPNKY
                     KEPKMTKCCCKKSMREALELLRYDSLRPFVNFNQFAFISDFFIVGAYLVGIDYSIPPK
                     DNLSGLNGTFERFSACNCDLIDISGQLYYPKPVPLTLGGLVTSIGTIPGVSELVALIE
                     AAVPATIDLGSILNSILDYIIDYIISTVNPLTNVDLASLCNLKAVAFNVTPADYEDFI
                     SSLAYYLDKKHYKECNCNCDCDDCCCNKGILDNLYMSNINNQVTVVAGSLVLTGVEVL
                     GKKNDVLVLGNSNDSRIYFVCVDSIDYIA"
BASE COUNT         1326 a          363 c          539 g         1118 t
ORIGIN      
        1 tatcaacaaa atatcaatga aattaaattt tctattggaa gtaaattata tttagaaata
       61 gtaaataatc aaagtacaaa tgaagatatt gaagaacatg ataattactc taactttatt
      121 atatatgata tagaagaaaa cacttttaat ataagtgata aatttatgga taatctaaat
      181 aaagaaaaga atgcaaatcc aaaagagatt ggtttattag gtaaaactaa gctagattat
      241 atggtaaaaa ataataaatt aaatcttata gttacagata tgagtaattc tataacaaat
      301 cttgtttatg atattaattc taataagatt aaattagaag attatgagat acttgattta
      361 aaagtggatt ataatgaagc atcatctgga attaggaaag ttaagttact ggatgataag
      421 atatattcta tttcaggtat aaagaataat ctatcaggag aattaaaagg gtttaaaggg
      481 gttgttgata tagttggaac taatccggta aaatttaaag tatttgatat gaaacttaaa
      541 aaaaccgttt atgaagctga gctaataaat ggagatgtaa gtttacaaga aaagttattt
      601 tttgttgcaa attaagtgat ggaattaatt ttataacttt aaacttttat atgtattgta
      661 ggtgaatata agtaaaggag tgtcttaaaa tgaatttttt agctcatgag acactctttt
      721 ttatatgaaa tcaaatatat ttttatcatt tagacaatga aaaataagat tatctctatt
      781 ttaagacaga catttgttta taattatata caaaaacatt ttttaatatt tttgtatagt
      841 atataataat ttaatttata ttaatacact taaatttgaa atagaatgag tataatggta
      901 aaattaatta ttagaatatt agaaataata aataaataat acattgtact taaaaggggg
      961 agaaatttaa gatgaacaaa aaatatatta aagcaggaaa tttaattgat ggaacaggaa
     1021 atagtaccat gcaaaataag ggtataatta ttgatggaga tactatagtt gaaattgaag
     1081 atataagaga agatttaaat ggctatgatg tgtatgatta cttagataaa actgttatgc
     1141 caggtattat gaattgtcat gttcacttga caatggagcc agttggaaat ccaaaagtat
     1201 attatgataa tgtatcagat gtagagcttg tagtaaatac gataaaacaa cttgatgctt
     1261 atttggattc aggagtaaca tatattagaa gtctaggttg cccaaaatat gtggatgtac
     1321 aacttaaaaa tcttatagac aaagggtcta tagatggtcc tggtatagtt acttcaggtc
     1381 cagttatatg catgacagga gggcatggat actattttgg tattgaaagt gatggagtgg
     1441 acgaatgtag aaaatcagcc agaacagttt taaaaaatgg agtagattgt gtaaagataa
     1501 tggcaactgg aggtgtaaca actgatgggg ttgaaccagg gtcaccacaa ttaactttgg
     1561 aggaaatgaa ggctgctgta gatgaagcta taaaggcagg caaaataact gccacacatg
     1621 cacaaggaag aactggtata aaaaatgcag tgcttgcagg aattacttct atagaacatg
     1681 gtgtttattt agatgatgaa ataattgatt taatgcttga aagaggaact tacttagttc
     1741 caactgttgc tgcaccatat tttattctaa atgcaggaaa agaaagtgga ataactgaag
     1801 ctactcttag aaagtgtgag attgttgcta ttccacacaa agagtcattt ctaaaagcat
     1861 ataaaaaagg aattaaaatt gcagttggtt ctgatgcagg aacttcttat aatgaacatg
     1921 gaaaaacata ttatgaaatg aaactaatgt cagattatgg catggataat atggatataa
     1981 tagttgctgc tacaaaaaca gcatctgaac ttttaagaat tgataaaaat tatggaactt
     2041 tagaaaaagg aaagaaagca gatgttatag ttgtagatgg aaatccaata gagaatatag
     2101 atgtacttgc agatgtgtta gcagtattta aacttggtaa aaaagttaga taagtaatat
     2161 aaaaaataaa aaagagtgtc taaagggatt tttaaataat tatagacact cttttttatt
     2221 agtaacaatt aagtctttta tactttaggg actagattct attttatatt tagatataac
     2281 tctttaaaat cattctatgc aatataatct atagaatcta cacatacaaa gtatattctt
     2341 gaatcattag aatttccaag tactagaaca tcatttttct tacctagaac ttcaacacca
     2401 gttagaacca aactaccagc tactacagta acttgattat ttatattaga catataaaga
     2461 ttatctagga tacctttatt acaacagcag tcgtcacaat cacagttaca attacattct
     2521 ttataatgct ttttgtcaag atagtaagct aaagatgata tgaaatcttc ataatctgca
     2581 ggtgtaacat taaaggcaac agctttaaga ttacataatg atgctaaatc tacatttgtt
     2641 aatgggttaa ctgtagaaat tatataatca attatataat caagtattga atttagtata
     2701 gaaccaaggt ctatagtagc aggaactgct gcttctataa gagcgactaa ttcggatact
     2761 cctgggatag taccaattga agtaactagt cctccaagtg ttaaaggaac aggtttagga
     2821 taatacaatt gaccagatat atcaattaaa tcacagttac aagctgaaaa tctttcaaaa
     2881 gtgccattaa gtccagataa attatctttt ggaggtattg aataatctat acctactaaa
     2941 tatgcaccaa caataaagaa atctgaaata aaagcaaatt gattaaaatt tacaaaaggt
     3001 cttagagaat catatcttaa aagttctaat gcctctctca tgctcttttt acaacaacat
     3061 tttgtcattt taggttcttt atatttattt gggtaattat agtatctatc agctacttga
     3121 taattttcat agtatctatc atttgcattc atataatctt cttcgtattc ttgagtaaat
     3181 ctaaagtcct ctctacattt attattttcc attaaataca ctccttttat attttgattt
     3241 aaatgtatag ataattaagt ttatctatat agtaatatta tatttattgt gtaaaaaaat
     3301 gttacaaaag aaattataaa tgaataaaaa ataataattt gtcaaa
//
LOCUS       DAFNNB010000227         3341 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0233, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000227 DAFNNB010000000
VERSION     DAFNNB010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0233"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            190..1269
                     /locus_tag="L3M60_003384"
     CDS             190..1269
                     /locus_tag="L3M60_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBN6228010.1"
                     /translation="MSKVKKVVELLESKGIDALYLTKKTNVNYISGFPDEEAYAVICK
                     YGNFLVTDSRYMELAENVCEGFEIINWHNFDRSVAKAVKSVCDKVGIKKLGFERTNIV
                     FDKYEELKNLIEKDNGELIPTENIVETLRYIKDEEEIKNTRKACEIADKALEELIPHI
                     KAGVSEIELATKLEYFMKMNGAQNIGFETILISGAKTSLLHGKPSDKIIEKGDFVLID
                     YGAMYNGYISDTTRTFIVGEASEKQLEIYNLVKEAQNVGVENMKVGVHATIPDAEIRK
                     VVKKYEDYYYQGIGHGVGRDVHEEPFIGNYGDKIIEDGCIITMEPGIYFPGWGGVRIE
                     DTVLITKNGPERLTKFPKDLMILDK"
     gene            1380..1979
                     /locus_tag="L3M60_003385"
     CDS             1380..1979
                     /locus_tag="L3M60_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228011.1"
                     /translation="MYKKHKFIKNIKQAWYKIKLIDRCLIIVMAILMFQSIYNLFVNE
                     VNSQDTTTIDVVVRTTSAAIFGYFLSVNFIKRPSRKYINTDVSSNFFIDNNLSKKSQE
                     SSQNNVMNVKNTIGFTSETDNYEKKIPTNNTEDFEDGETSELQIVIATVICVVALTVL
                     FVVRNFTTTTSASLGTISQMRDFVSGCVGFLLGCPSRPD"
     gene            complement(2159..3031)
                     /locus_tag="L3M60_003386"
     CDS             complement(2159..3031)
                     /locus_tag="L3M60_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBN6228012.1"
                     /translation="MNINYLYYFQTVCKYKNMTKAAESIHISQPSITLAIKELEKELG
                     FELFYRIGNKIELTPEGKIFLDKSKHFIKQFEDFQCDALDLGKKRKASLKIGIPTVLG
                     TFLLSKILPRFNVIYPDIELKIFEVPTFVGAKMIEESTLDFCIGIIDSDIYDDIDSKT
                     IYKTELYLVTNPKNELAKHPIISSYMLKNVPFVILSEGSYHYKIITKRLEKAKPNIIL
                     HSNQLSTIRYLLENDLASTILYKEIFQNTKNLCSIPLERAITANIGVLWRRNQYISHS
                     MKLFIEYMASIHIN"
BASE COUNT         1299 a          354 c          523 g         1165 t
ORIGIN      
        1 ttttctagct aatttatcat atttgttcaa ttgaatttaa gtttttagct aaataaaact
       61 aataaaatat taaagtaaaa ttaataaaat ctgaaaaaat tttagaaata aactatctat
      121 tagataaaaa gaaggctaat tagtgatata atataattag gataaaattt attatttgga
      181 ggcaaaatca tgagcaaagt aaaaaaagta gtagaattgt tggaatctaa aggtattgat
      241 gcactatatc taacaaagaa aactaatgta aactatataa gtggttttcc tgatgaagaa
      301 gcatatgcag tgatttgtaa atatggaaac tttttagtta ctgatagtag atatatggag
      361 cttgcggaaa atgtttgcga aggttttgaa attataaatt ggcataattt tgatagaagt
      421 gttgcaaagg cagttaaaag tgtatgtgat aaagtgggaa taaagaaact tggatttgaa
      481 agaactaata tagtgttcga taaatatgaa gaacttaaaa atctgattga gaaggataat
      541 ggagaattaa tacctacgga aaatattgta gagactttga gatatataaa agacgaagaa
      601 gaaatcaaaa atacaagaaa agcttgtgaa atagcagata aagcattaga agaattgatt
      661 ccacatataa aagctggagt tagcgaaatt gaactggcta cgaagttaga gtactttatg
      721 aagatgaatg gagcacaaaa tataggattt gaaactatat tgatatcagg agcaaagact
      781 tcattgcttc atggtaagcc tagtgataag attatagaaa aaggtgattt tgtacttata
      841 gactatggtg ctatgtataa tggttatata tctgacacaa ctagaacttt tatagttggg
      901 gaagcttctg aaaagcaatt agaaatatat aatttagtta aggaagccca aaatgtaggg
      961 gttgaaaaca tgaaagttgg tgtacatgcg acaattccag atgctgagat aagaaaagtt
     1021 gtcaaaaaat acgaagacta ttactatcag ggtataggac atggagtcgg tagagatgtt
     1081 catgaagaac ctttcattgg taattatggt gataaaatca tagaagatgg atgtataata
     1141 acaatggaac caggtattta tttccctgga tggggtggag ttagaataga agacactgtt
     1201 ttgattacta aaaatggacc agaaagactt actaaattcc ctaaagattt gatgatttta
     1261 gacaaataat ttaatttata tatattttta cataaaatat aacataatct cactattaag
     1321 ccctatttac atattatgat aaagagggct taattttatt gtgagaggtg ataatgtcca
     1381 tgtataaaaa acataaattt ataaaaaata taaaacaagc ctggtataaa atcaaactaa
     1441 ttgatagatg ccttataatt gtaatggcaa tattaatgtt tcaatctata tacaatttat
     1501 ttgttaatga agtaaattca caagacacta caactattga tgtagtagta agaactactt
     1561 cagcagctat atttggatac tttttaagtg ttaattttat aaagagacct tcaagaaagt
     1621 atataaatac tgatgtatct tcaaattttt ttatagataa taatttatct aagaaaagtc
     1681 aagaatcaag tcagaataat gtaatgaatg tcaaaaatac cattggattt acttcagaaa
     1741 ctgataatta tgaaaagaaa atacctacta ataatactga agattttgaa gatggagaga
     1801 ctagtgaatt acaaattgtt atagctactg taatttgtgt agtagcatta acagtacttt
     1861 ttgttgtaag aaattttaca actacaacat cagcttcttt aggtactatt tcacagatga
     1921 gagattttgt ctcaggatgc gtgggttttt tactaggttg tccaagtaga cctgattaaa
     1981 aatgtgatta ctaataaaaa agattgttaa aagtatcagt ataataatac ttagacatta
     2041 aggcatataa ttataatctt ataagatttg atatcctatt tttatactga tactctaaaa
     2101 tatttattaa aacattttta atattacatt taaataaaat attatggcta cataaaagtt
     2161 aatttatatg aatagatgcc atatattcaa taaaaagttt catactatga gatatgtatt
     2221 gatttcttct ccaaagaacc ccaatatttg cagtaattgc acgctctaat ggtattgaac
     2281 agagattttt tgtattttga aatatttctt tatatagtat tgttgaagcc aagtcatttt
     2341 ctagtaaata acgtatggta gaaagttggt ttgagtgtaa aattatattt ggcttagctt
     2401 tttcaagtct ttttgtaata atcttatagt gataagaacc ttcagataag attacaaaag
     2461 gtacattttt taacatataa cttgatataa tggggtgttt tgccaattca tttttgggat
     2521 ttgtaactaa atataattct gtcttataga ttgtctttga atctatatca tcataaatat
     2581 cactatcaat tataccaata caaaaatcaa gtgtagattc ttcaatcatt ttagctccaa
     2641 caaatgttgg aacttcaaaa attttaagct ctatatctgg ataaattaca ttaaatctag
     2701 gaagtatttt cgaaagtaaa aatgtaccta aaactgtagg aatacctatt ttgagagaag
     2761 cttttctctt ttttcccaaa tccagtgcgt cacattgaaa atcctcaaat tgttttataa
     2821 aatgttttga cttgtctaaa aagatttttc cttcaggcgt taactctatt ttattaccta
     2881 ttctataaaa aagttcaaag ccaagttctt tttctaattc tttaattgct aatgttatgg
     2941 atggttgaga aatgtgaata ctttccgcag ccttagtcat gtttttatat ttacagacag
     3001 tttgaaaata gtataaatag tttatattca tttggtatta cttcctttca tttacgtatt
     3061 agtgacaagt atataattaa caatagtata agttttgtat attgttgtaa aaaaatagaa
     3121 tagttttaaa ctatcatatc ataagttttt gatattttca atctattgag aatagtgtta
     3181 ttattaaatt aacaaaagag aatagctgat atggtttact ctaaatcaat ctataataca
     3241 gttatatcaa cgaatattaa aaaaatatct attaaaaaat aaaaaatatt tttacaatta
     3301 aattgttaat taaatcgtaa agaaaaaaat gatattatca a
//
LOCUS       DAFNNB010000228         3316 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0234, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000228 DAFNNB010000000
VERSION     DAFNNB010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3316
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0234"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            320..466
                     /locus_tag="L3M60_003387"
                     /pseudo
     CDS             320..466
                     /locus_tag="L3M60_003387"
                     /inference="COORDINATES: protein motif:HMM:NF013180.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            556..654
                     /locus_tag="L3M60_003388"
     CDS             556..654
                     /locus_tag="L3M60_003388"
                     /inference="COORDINATES: protein motif:HMM:NF013180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBN6228013.1"
                     /translation="MSVSIGISLYSKYGEQFYKLYRHADSALYRVK"
     gene            852..2672
                     /locus_tag="L3M60_003389"
     CDS             852..2672
                     /locus_tag="L3M60_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HBN6228014.1"
                     /translation="MKKKYDFLIYAAIWLVAIIIFIFSLYHSIEHIKIINYTGIISSE
                     TQKVTKQELNNKRNDKLISQLDSILIELRTGNGKHRLQRCDNKEFQEKLIEMDAMWDS
                     MKKEIRKVRNGGSGDKLYKLSEKYSSLSNQVVFISEKHSDIKLHFFATVLFIYLIIST
                     ISLLIWEYYNKRKFDKIFYTDNLTKIKNQVAFENRAIEILSHAYNKEYVLLNIDIDNF
                     KYINDTHGYEYGDKVLVIVANVLAKTFNTKETCARVGSDNFVILAKYRESLLYEISKT
                     LTDAIISELNMNVTQTISFCIGAYLVEIEKSNYKYINSIMDKANIAHKVSKMRGISST
                     VWYNENLLKQLQMENSIYNHMYKALENEEFQMYLQPKFQISSLNVVSAEALVRWFSPE
                     LGFLSPDQFIPLFEKSGFIIELDFYMLKKACSFIKKTFMKKNKYTYPIAVNFSRVTIY
                     QNDFYQRFLDTVREYEIPFKYIEIEVTESAFNEISEPVISILDKLEKLGFLISMDDFG
                     SGYSSLSLLCSLSINGLKLDKSLLKEKFNRAKVYSIIKCIIEMSHSIDMSIVCEGIET
                     KKDLEFLKTVKCDLGQGFYFSKPIEEKEFFNKYVMDKQYN"
     gene            complement(2757..>3316)
                     /locus_tag="L3M60_003390"
     CDS             complement(2757..>3316)
                     /locus_tag="L3M60_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228015.1"
                     /translation="QFERSYDIGIFKNEQSIHKFIEKIPFVKKDSSNFNYTNYYMEFE
                     DIPEYYEVNFNNYLYIITKFSFIPDGTEIFFTWDEIHCWDEKVATDKTFINGETLVDS
                     YSFPNNEVKNYIQKRESLYRETKKYYKEKGLKIKRHALGSEDGEYVELVDDSILYLLD
                     VHAVEIWEQSKNIEEFLYKYKKYFE"
BASE COUNT         1282 a          333 c          468 g         1233 t
ORIGIN      
        1 agaactgttt tcaaaaagta tttttttgtt taataatata aatattatta actttattat
       61 ccttttataa tctatataac taaatataac taatttatta tctatttata aattttattt
      121 ttagacataa ttaataagag ataagtagat tattgtattt attgtataaa cataggcgta
      181 attcaagtaa aatttcaaaa aaaaggaata tatttccata atatgataaa ataaattcaa
      241 agagttaaat tctagtcaaa tattacttta attatttatt aaaatagatt agatattcca
      301 tattcaatac cattgagttg atgtgtataa ttttaaatat gtaaaggatt ctatggggta
      361 tgtatttgga gataaaatat taattgattt agctgaatgt ttaaaaagat attttagaga
      421 aacggacact gttggtcgtg taggaggtga tgagtttata atatagtgaa gaaagagaaa
      481 gtgtaaaaaa taaaggcttt gataatatta aaaaggctgg acaataagtt tacgtatgat
      541 gaatgatatt ttgagataag cgtaagtata ggaatttctt tatattcaaa atatggagaa
      601 cagttttata agttatatcg tcatgcagat agtgctctat atagagtaaa ataagaagga
      661 aaaaacacgt attgtatgtt tttctatcta aaaataaaat ctatattata aaggattgtt
      721 ctatcttagg tatataaatg attgagatat ttaagataaa tacatattac tgcaaagcat
      781 tgataattat ttttgatgag agattgatta tttgcaaatt atatagatac attataattg
      841 agggaataat aatgaagaaa aaatatgatt ttttaattta tgctgctatt tggctagttg
      901 ctattattat ttttatattt tctctatacc atagtataga gcatattaaa attataaatt
      961 atactggtat tattagcagt gaaactcaaa aagttactaa acaagaatta aataataaaa
     1021 gaaatgacaa attgataagt caactagata gtattttaat agaactaagg acaggcaatg
     1081 ggaaacatag gttgcaaaga tgtgataata aagaatttca agaaaaatta attgaaatgg
     1141 atgctatgtg ggattctatg aaaaaagaaa ttagaaaagt tagaaatggt ggttcaggtg
     1201 ataaattata caaattgagc gaaaagtatt cttcgttgtc taatcaagtt gtttttatat
     1261 ctgaaaaaca ttctgatata aaactgcatt tttttgccac tgtattattt atttatctta
     1321 taatttcaac aataagtctc ttgatttggg aatattacaa taaaagaaaa tttgacaaaa
     1381 tattttatac tgataatttg acaaaaataa aaaaccaagt tgcatttgaa aatagagcta
     1441 ttgaaatttt atctcatgcg tataataagg agtatgtgct actaaacatt gacattgata
     1501 attttaaata tattaatgat acacatggat atgaatatgg tgataaagtt ttagtaatag
     1561 ttgcaaatgt acttgccaaa acttttaata ctaaagaaac ttgtgcaaga gttggttcag
     1621 ataattttgt aatacttgca aaatatagag aatctctctt atatgagatt agtaaaactc
     1681 taactgatgc aataatatcc gaattaaata tgaatgtgac acaaactata tctttttgta
     1741 taggagcata tttagttgaa attgaaaaat caaattataa atatataaat tctataatgg
     1801 ataaggcaaa tattgcacat aaagtaagta aaatgagagg aatttcatct acagtgtggt
     1861 ataatgaaaa tctattaaag caattacaaa tggaaaacag tatctataat catatgtata
     1921 aagcactaga gaatgaagaa ttccagatgt atttgcaacc aaaatttcaa atatcttctt
     1981 taaatgttgt aagtgcagaa gctcttgtac gatggttttc tccagaactt ggttttttgt
     2041 caccagatca attcattcca ctatttgaaa aaagcggatt tattattgaa ttagactttt
     2101 atatgttaaa gaaagcttgt tcattcataa aaaaaacatt tatgaaaaaa aataaatata
     2161 cttatcccat agcagtcaat ttttcaagag ttactattta tcaaaatgat ttctaccaaa
     2221 ggtttttgga tactgttaga gaatatgaaa ttccttttaa gtatatagaa atagaggtaa
     2281 ctgaaagtgc ttttaatgaa atatctgagc cagttatttc aattttagat aagcttgaaa
     2341 agttaggttt cttaatttct atggatgatt ttggctcagg atactcgtca ttaagccttt
     2401 tatgttcttt atcgattaat ggattaaagt tagataaaag cttacttaaa gagaaattta
     2461 atagagcgaa agtatatagt attattaagt gtattatcga aatgtcacat agtatagata
     2521 tgtctattgt atgtgaaggt attgaaacta aaaaagattt agagttttta aagacagtta
     2581 agtgtgatct agggcaagga ttttattttt ctaaacctat agaagaaaaa gaatttttta
     2641 ataaatatgt aatggacaaa caatataatt agttttattg ttaatcttat gcattgagtt
     2701 attttatatc ttattaaata gctcaatgca tagttatgca ggtcaaaaaa cataaatcat
     2761 tcaaaatatt tcttatattt atataaaaat tcttctatat tttttgattg ttcccatatt
     2821 tcaactgcat gtacatcaag taaataaagt atggaatcat ccacaagttc aacatattcg
     2881 ccatcttctg aaccaagtgc atgtcttttg attttcaatc ctttttcttt atagtatttt
     2941 tttgtttctc tatatagtga ttctcgtttt tgtatatagt tttttacttc attatttgga
     3001 aaactatatg aatctaccaa tgtctcacca tttataaaag ttttatcagt agcaactttt
     3061 tcatcccagc aatgtatttc atcccaagta aaaaatatct cagttccatc tggaataaat
     3121 gaaaatttag taataatata caaataatta ttgaaattta cttcataata ttcaggaata
     3181 tcttcaaatt ccatatagta atttgtataa ttaaagttac tactatcttt ttttacaaat
     3241 ggtattttct caataaattt atgtatagat tgttcatttt taaaaattcc tatatcatag
     3301 cttctttcaa attgat
//
LOCUS       DAFNNB010000229         3276 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0235, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000229 DAFNNB010000000
VERSION     DAFNNB010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3276
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0235"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            591..2075
                     /locus_tag="L3M60_003391"
     rRNA            591..2075
                     /locus_tag="L3M60_003391"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2384..2460
                     /locus_tag="L3M60_003392"
     tRNA            2384..2460
                     /locus_tag="L3M60_003392"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2418..2420,aa:Ile,seq:gat)
     gene            2478..2553
                     /locus_tag="L3M60_003393"
     tRNA            2478..2553
                     /locus_tag="L3M60_003393"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2511..2513,aa:Ala,seq:tgc)
     gene            complement(2601..2888)
                     /locus_tag="L3M60_003394"
     CDS             complement(2601..2888)
                     /locus_tag="L3M60_003394"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228016.1"
                     /translation="MDNQTFSNNRFDKKDQLLEINPMALRLSGQSSARAFDEHPLRHF
                     PAFMAQRPADPHQKVQTLEHPEQSEFIFSSRFHTEQATERLKASQAANLLL"
     gene            2950..>3276
                     /locus_tag="L3M60_003395"
     rRNA            2950..>3276
                     /locus_tag="L3M60_003395"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          756 a          758 c         1008 g          754 t
ORIGIN      
        1 atccggcatg tttgtggccg cagccatcac aatacgcttc ggcggcttat ttgccggcgt
       61 ttcgtccagc aaatacgggg ctgtggataa atcgtgtcac atttttgata aaaaacgcaa
      121 aaggcgtgtt gacagttttg gttggtgggg tctatatacc gacacaacga gggcggggcg
      181 ccgctggcgg ttgctgaatg cgccctaaag gaagctctct ggtggttttc ggattgttgc
      241 gcgagtgacg atccttcgag tagggcctcg aagggattga cggtgtggcc ttgtggtcgt
      301 gcggttggtt ttcggggttg gacttggtgc cggctggttc cggttgacgg tggtttttgc
      361 catggtctgt tctttgacaa ttttatacag aagaaagaga aacgtgggcg gcagggtcct
      421 gcggaacatc ggaagatgtt caacagatac tttggcggac acgtttcgag agaagatgtt
      481 acatgcgtct gagcaatcag atgtaaggtg tatatatgtt ctcgtcgatt caaaggattg
      541 aggcttcggc tttgatccaa gcgtgaccaa tatagccaat atcaaatttt caacttgaga
      601 gtttgatcct ggctcagaac gaacgctggc ggcaggctta acacatgcaa gtcgagcgcc
      661 ccgcaagggg agcggcagac gggtgagtaa cgcgtgggaa tctacccatc tctacggaat
      721 aacgcatgga aacgtgtgct aataccgtat acgtccttcg ggagaaagat ttatcggaga
      781 tggatgagcc cgcgttggat tagctagttg gtggggtaaa ggcctaccaa ggcgacgatc
      841 catagctggt ctgagaggat gatcagccac actgggactg agacacggcc cagactccta
      901 cgggaggcag cagtggggaa tattggacaa tgggcgcaag cctgatccag ccatgccgcg
      961 tgagtgatga aggccctagg gttgtaaagc tctttcaccg gtgaagataa tgacggtaac
     1021 cggagaagaa gccccggcta acttcgtgcc agcagccgcg gtaatacgaa gggggctagc
     1081 gttgttcgga tttactgggc gtaaagcgca cgtaggcgga ctattaagtc aggggtgaaa
     1141 tcccggggct caaccccgga actgcctttg atactggtag tcttgagttc gagagaggtg
     1201 agtggaattc cgagtgtaga ggtgaaattc gtagatattc ggaggaacac cagtggcgaa
     1261 ggcggctcac tggctcgata ctgacgctga ggtgcgaaag cgtggggagc aaacaggatt
     1321 agataccctg gtagtccacg ccgtaaacta tgagagctag ccgtcgggca gtatactgtt
     1381 cggtggcgca gcaaacgcat taagctctcc gcctggggag tacggtcgca agattaaaac
     1441 tcaaaggaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgaagcaac
     1501 gcgcagaacc ttaccagccc ttgacatccc gatcgcggtt accagagatg gtttccttca
     1561 gttaggctgg atcggtgaca ggtgctgcat ggctgtcgtc agctcgtgtc gtgagatgtt
     1621 gggttaagtc ccgcaacgag cgcaaccctc gcccttagtt gccatcattc agttgggcac
     1681 tctaagggga ctgccggtga taagccgaga ggaaggtggg gatgacgtca agtcctcatg
     1741 gcccttacgg gctgggctac acacgtgcta caatggtggt gacagtgggc agcgagaccg
     1801 cgaggtcgag ctaatctcca aaagccatct cagttcggat tgcactctgc aactcgagtg
     1861 catgaagttg gaatcgctag taatcgtgga tcagaatgcc acggtgaata cgttcccggg
     1921 ccttgtacac accgcccgtc acaccatggg agttggtttt acccgaaggt gctgtgctaa
     1981 ccgcaaggag gcaggcaacc acggtagggt cagcgactgg ggtgaagtcg taacaaggta
     2041 gccgtagggg aacctgcggc tggatcacct cctttctaag gggacgcgtg aacaggcaac
     2101 agttaggctg gtctttcaca tgtctattag aacaaaggtt gcaagccagt cagcttgaca
     2161 accgtgcata caatgcaggg actgccgcct acgtttctct ttctttgatg tttttgtaag
     2221 cgctcacgca cgtgcggtcc tttggacctg tgctccgcga gggcgcggca cgagccgcga
     2281 cggccggtcg gcctactttg gtgacctacc gtctaaggac gggcgcacgg cacgagctgc
     2341 gacgcgtaag cgcgctgcat ttgacggttg atccggcaaa tcagggcttg tagctcagtt
     2401 ggttagagcg cgcgcttgat aagcgtgagg tcggaggttc aagtcctccc aggcccacca
     2461 tattcctata cctttacggg ggcgtagctc agctgggaga gcacctgctt tgcaagcagg
     2521 gggtcgtcgg ttcgatcccg tccgcctcca ccaaggttct tggcaaggtt cttggcagat
     2581 ggacggagca aaaacatcat ctaaagaagc aagtttgcag cttgtgaggc cttgagcctt
     2641 tcggttgcct gttctgtatg aaatcgtgaa gagaagataa attcggattg ttccggatgt
     2701 tcaagggttt gcaccttctg gtgcgggtcc gccggccttt gggccatgaa cgccggaaaa
     2761 tgtcgcaagg gatgctcatc aaaagccctt gcagaggatt ggccgcttaa ccgcagagcc
     2821 atcggattta tctcgagaag ctggtctttt ttgtcaaatc gattgttgct gaatgtttgg
     2881 ttgtccattg gtatccccca tggcggcgaa atgttcgtga aggcaaggat ggatattggc
     2941 aatgagaatg atcaagtgtc ttaagggcat ttggtggatg ccttggcatg cacaggcgat
     3001 gaaggacgtg atacgctgcg ataagcgtcg gggaggtgcg aatacccttt gatccgacga
     3061 tttccgaatg gggaaaccca ccttagatat ctagaaaatt attttggttg gaagattgat
     3121 cacaaccatt ggacgggctg ccttcccaga aggacgcgcc catacagacc gctcggtcgt
     3181 cggccacttt tggccgcccc agcggagcgc cagtccgaag gacgtacggc gcgtgagcga
     3241 tcggtagaac caaaataatt tctagatatc aaaata
//
LOCUS       DAFNNB010000230         3268 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0236, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000230 DAFNNB010000000
VERSION     DAFNNB010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0236"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            25..846
                     /locus_tag="L3M60_003396"
     CDS             25..846
                     /locus_tag="L3M60_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HBN6228017.1"
                     /translation="MNLIDNHIHTNFSSDGKDSMEDTIKKAISIGVRYLTFTDHLEHD
                     EDRGFCINYNDYVPVFNKFKEKYKKDIELLLGVEVGYRKHLKNEIEEIINSNPFDFVL
                     CSTHTIDNIPVPSKAYFKGLSKEDAYYKYFNSILETNREFRDYNIYGHLDYISRYGIY
                     SDNRVIYNDFKDIIDEVLKSIINNGSGIELNTSGYRYGLNAIHPNEDILKRYKELGGF
                     IVTVGSDSHRVEDICKDFDVAYDMLKYLDFKYVSLFKERETYFINIEKVKSNNIA"
     gene            1104..2987
                     /locus_tag="L3M60_003397"
     CDS             1104..2987
                     /locus_tag="L3M60_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HBN6228018.1"
                     /translation="MSNKNKDNITLSNQIKRTTQNSLTKEAILHIPMSNYAYGYDRET
                     LHIRLRTKKNEAKRVILRIGDQYVWDKGGAGGGNLNASGVGWSGGTNIIMNKEVETEL
                     FDYWIAKCKPLNKRSRYGFIIEGQKEKILFTEKRIIELGNENDEKELCEISNFFGYPY
                     LNYIDIPKVPNWVKDTVWYQIFPDRFANGDSSINPEGTDEWGATPTRENFTGGDLQGV
                     IEHLDYLSDLGISGLYFCPITIGKTNHRYDTIDYMEIDPALGDKRTLKKLIEEAHKRN
                     IKIMLDAVFNHIGYYSKQWQDVVQNKENSRYKDWFYIKDMNKIDIPIEQIDEKNIPYE
                     TFGCEKYMPKLNTENSEVIEYLLDVGKYWIHEFDIDAWRLDVSNEVDHAFWRKFREEV
                     KKVKPDIYILGEIWHGSLPWLMGDQFDSVMNYLMSEAMKKFFCTDEINAEEFKYMIND
                     VMVSYPIQVNEVIFNLLGSHDTTRILTYANGNVDRFKLAYMFMFVQTGCPCIYYGDEI
                     GMEGELSSISEGQRKCMEWNEEKWNRDIFNFMKKIIRLRKENKELRSISNEWVLADKE
                     NGAIILKKESIFIIINNSSKDIILTLPDYLANKKVKDLYEEEIVDLKEELMLEKYKFI
                     ILK"
BASE COUNT         1368 a          276 c          570 g         1054 t
ORIGIN      
        1 agataaaata aagggaggga taatatgaat ttaatagata atcatattca tactaatttt
       61 tcaagtgatg gaaaggattc aatggaagat acaataaaaa aagctataag tattggtgtt
      121 agatatttaa cttttacaga ccatttagaa catgacgaag atagaggatt ttgcataaat
      181 tataatgact atgttccagt gtttaacaag tttaaagaaa aatataaaaa agacatagag
      241 ttattattag gtgttgaagt aggttataga aaacatctaa aaaatgaaat agaagaaata
      301 ataaattcta atccttttga ttttgtattg tgctcaactc atacaattga caatatacct
      361 gttccatcaa aggcatattt taaaggactt agcaaagaag atgcgtatta taaatatttt
      421 aatagcatac ttgaaactaa tcgtgaattt agagattata atatatatgg tcatttagat
      481 tatatatcta ggtatggaat atattcagat aatagagtta tatacaatga ctttaaagat
      541 attattgatg aggttttaaa atcaataatc aataatggta gtggtataga gctaaataca
      601 tctggttata gatacggact gaatgctata catccaaatg aagatattct aaaaagatat
      661 aaagaacttg gagggtttat agttactgta ggttctgatt ctcatagagt agaagatata
      721 tgcaaagatt ttgatgtggc ctatgatatg cttaagtatc ttgactttaa atatgtatct
      781 ttatttaaag aaagagaaac ttattttata aatatagaaa aagttaaatc taacaatata
      841 gcttagagtt tattttgaag taaattaatg gatagttaaa aagtcaatag ttataatatt
      901 gtatcctcta gaaaaatact agaggttttt attttgcaaa aataactaaa tagaattaaa
      961 tttaaacaat atagtttatt ttaaattgaa tagtttattg ttgatatatt ttactattat
     1021 atatttttat tgtgatataa tagtaaaata tattaaccta tctttagatt atataaatta
     1081 ttataaagga gggaaatttc aaaatgtcta ataaaaataa agataatata actttaagta
     1141 atcaaatcaa aagaacaact caaaatagtt taacaaaaga agctatatta catataccaa
     1201 tgagtaatta tgcctatggt tatgatagag aaacactaca tataagactt agaactaaaa
     1261 aaaatgaagc taaaagagta attttacgaa taggagacca atatgtttgg gataaaggtg
     1321 gtgcaggagg aggaaattta aatgcatctg gagttggttg gtctggtgga acaaatataa
     1381 taatgaacaa agaagtagaa actgaattat ttgattattg gatagctaag tgtaaacctt
     1441 taaataagcg ttcacgatat ggctttataa tagaaggcca aaaagaaaag attttattta
     1501 cagaaaaaag aataatagaa ttagggaatg aaaatgatga aaaagaatta tgtgaaataa
     1561 gtaatttttt tggatatcca tatttaaatt atatagatat acctaaagtg ccaaattggg
     1621 tgaaagatac tgtttggtat cagatatttc cagatagatt tgcaaatgga gattcatcta
     1681 taaatccaga aggaacagat gaatggggag ctactccaac tagagaaaat tttacaggtg
     1741 gagatttaca aggtgtgata gagcacttag attatttgag tgatttgggg ataagtggac
     1801 tttacttttg ccctattaca attggaaaaa ccaatcatag atacgataca atagattata
     1861 tggagataga ccctgcctta ggggataaaa ggactttaaa aaaactaata gaagaagcac
     1921 ataagagaaa tataaaaata atgttggatg ctgtttttaa tcatatagga tattattcta
     1981 aacagtggca agatgtagtt caaaataagg aaaattctcg atataaagac tggttttata
     2041 taaaagatat gaataaaatt gatattccaa tagaacagat agatgaaaaa aatatacctt
     2101 atgaaacctt tgggtgtgaa aaatatatgc ctaaacttaa tacagaaaat tcagaagtaa
     2161 tagaatattt attggatgta ggtaagtact ggatacatga atttgatata gatgcatgga
     2221 gattagatgt ttcaaatgaa gtagaccatg ctttttggag aaagttcaga gaagaagtta
     2281 aaaaagtaaa accagacatc tatatattgg gagaaatttg gcatggtagt ttaccatggc
     2341 taatgggtga ccaatttgat tcagttatga attacttgat gtcagaagcc atgaaaaaat
     2401 tcttttgtac agatgagata aatgctgaag agtttaagta tatgataaat gatgtaatgg
     2461 taagttatcc aatacaagtt aatgaagtta tttttaattt acttggaagt catgatacta
     2521 caagaatatt gacttatgct aatggaaatg ttgatagatt taaacttgca tatatgttta
     2581 tgtttgtaca aacgggatgt ccttgtatat actatggtga tgaaattgga atggaaggag
     2641 aattaagttc aatatctgaa ggacaaagaa aatgtatgga atggaatgaa gaaaaatgga
     2701 atagagatat atttaatttt atgaagaaga ttattaggct tcgtaaagaa aataaagagc
     2761 ttaggtctat atcaaatgaa tgggtgttag cagataagga aaatggagca attatattaa
     2821 aaaaagagtc catttttata ataataaata attcatctaa agatataatt ttgacattac
     2881 cagattattt agcaaataaa aaagtaaaag atttatatga agaagaaata gttgatttaa
     2941 aagaagaact aatgttagag aaatacaagt tcattatttt aaaataatac aaaagggtat
     3001 ttattttaga ttttatgtta aattattata cagtttaaac aaatctttaa gaagataaaa
     3061 aaatatggtc tataagggaa tgaattagaa gtagatttta aattaaagac tagattagaa
     3121 tagataattt tataaataat atcttaaata caagaaagaa aataaattga ttattagtct
     3181 aaaaaattct tgtagaaaga gggaataaga atgattgaag catttatatt tgattaaaaa
     3241 atagctacac acacttgtga ctttagtc
//
LOCUS       DAFNNB010000231         3141 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0237, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000231 DAFNNB010000000
VERSION     DAFNNB010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0237"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(9..707)
                     /locus_tag="L3M60_003398"
     CDS             complement(9..707)
                     /locus_tag="L3M60_003398"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HBN6228019.1"
                     /translation="MNEKLIGILAGMGPRSTAPFVDLIIDECQSQYGAKYDDEFPKMM
                     IYSIPTPFYIDRPINHELMKETIIDGLQKLESTGVNFIAMPCNSAHIYFKELKKSINI
                     PLLNIVEETVKKLPTLSQKVTLFSTSSTFESTIYQNGIIHNEHEFIFKDEWQIKLNNL
                     IQNIKIDKENPHNINIWNKLIEDVKNESIENIVIACTDLNVVLEKSYPSINIVDSSKC
                     LAESVVNKYLKLVK"
     gene            complement(869..2086)
                     /locus_tag="L3M60_003399"
     CDS             complement(869..2086)
                     /locus_tag="L3M60_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HBN6228020.1"
                     /translation="MLGKKCMDYLQTLGKISSTTNGLTRLILTQEHKKSIDLISSWMK
                     DLNLDIEIDDIGNVIGTYKSSFPNAPTLVVASHQDSVKCGGIFDGMLGIIVPLVGLEE
                     AKHNNKSYPFNIKLIAFAEEEGTRFETSLMGSKVFAGTFKEELLKSVDENGITLEEAV
                     TNFGFNTKNLTNLHPRKDIDAYLEFHIEQGPVLENESLPAGIVSSITGFKSFKISVNG
                     KSGHAGTLPMNMRLDAGCCACECVLAIEKVAKTTADLVATVGKMNFYPSSSNVVPEKA
                     EFTLDVRSCSQEILDNSVEKIFNEISHICENRKLNYTSELAFENVPVPCSNKITKIIE
                     KSFIDLNLNPFYIYSGAGHDAQEMDNITDIGMVFIRCAGGVSHNPNESVSVDDLDTAV
                     KIFLKILDNLDLK"
     gene            complement(2278..2691)
                     /locus_tag="L3M60_003400"
     CDS             complement(2278..2691)
                     /locus_tag="L3M60_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6228021.1"
                     /translation="MELEITEKIEKQDEDTIFQGLLKYNLARLEDKNPVDLGIYIRDK
                     AGKIVAGFIGVTHGNWLSIKYLWVSEKLRYKGTGSQLLYKAEKIAKERGCKYVFLDTF
                     SFQAPKFYEKFGYHKVFSLENYPLTGKRYYFRKIL"
BASE COUNT         1130 a          437 c          354 g         1220 t
ORIGIN      
        1 catgaaattt atttaactaa ttttagatat ttattgacaa ctgattcagc caagcatttt
       61 gatgaatcaa caatatttat tgaaggatat gatttttcta aaactacatt taaatcagta
      121 caagcaatta ctatattttc tatagattca tttttaacat cttcgataag tttattccaa
      181 atattaatat tgtgtggatt ttctttatct atttttatat tttgaataag attgtttaat
      241 tttatttgcc attcatcttt aaatataaat tcatgttcat tatgtattat accattctgg
      301 taaattgtag actcaaaagt tgaactagtc gaaaaaagag ttactttttg tgagagagta
      361 ggtaattttt taactgtttc ttctacaata tttaatagtg gtatattaat agactttttt
      421 aattctttaa agtatatatg tgctgaattg catggcattg caataaaatt tacaccagtt
      481 gactcaagtt tttgtaatcc atcaattata gtttctttca taagttcatg atttattgga
      541 cggtcaatat aaaatggggt aggtattgaa tatatcatca tctttggaaa ttcatcatca
      601 tattttgctc catattgaga ttgacactca tcaataatta aatcaacaaa aggggctgtt
      661 gaccttggac ccattccagc caaaatacca attaattttt cattcataaa aagtatctcc
      721 tttatacata aatatattta tgtataaaat aaaaatttat aaaaacataa aattatattt
      781 tataatagat tatattatac acttgtataa tataagtata aagtgagttt ttagattata
      841 tattttaata tactttctta tataaaaatt attttaaatc aagattatct agtattttta
      901 aaaaaatttt tacagcggta tccaaatcat ctacagaaac actttcatta ggattatgac
      961 ttacgccccc agcgcatctt ataaatacca taccaatatc agttatatta tccatttcct
     1021 gagcatcatg accagcacca ctataaatat aaaaaggatt aagatttaaa tctataaaac
     1081 ttttttctat gatttttgtt attttattag aacatggtac aggaacattt tcaaaagcaa
     1141 gttcacttgt atagttaagt tttctatttt cacatatatg tgatatttca ttaaatatct
     1201 tttctacaga attatcaaga atttcttgag aacaagagcg tacatcaaga gtaaattcag
     1261 ctttttcagg taccacattt gatgaagatg gataaaaatt catctttcct acagtagcaa
     1321 ccaaatcagc agttgtctta gctacttttt cgatagctaa gacacattca caggcacaac
     1381 atccagcatc aagtctcatg ttcattggga gtgtaccagc atgtccagat ttaccattta
     1441 ctgatatctt aaaacttttg aatcctgtaa tacttgatac aattccagca ggaagagatt
     1501 cattttctaa gacaggtcct tgctcgatat gaaattctaa atatgcatct atatcttttc
     1561 taggatgcaa atttgttaag ttttttgtat taaatccaaa atttgttaca gcttcttcaa
     1621 gtgtaattcc attttcatca acagatttaa gaagttcctc tttaaaagta ccagcaaaaa
     1681 ctttacttcc cattaaagaa gtttcaaatc tagttccttc ttcttcagcg aaagcaatta
     1741 gtttaatatt gaaaggataa gacttattat tgtgttttgc ttcttcaaga ccaactagag
     1801 gcactattat gcctaacata ccatcaaata tacctccaca ctttacacta tcttgatgag
     1861 atgcaactac taatgtggga gcatttggaa agcttgattt atatgtacct ataacatttc
     1921 caatgtcatc aatttcaata tcaagattta aatctttcat ccatgaactg attaaatcaa
     1981 tagatttttt atgttcttga gtcaagataa gtcttgtaag accatttgtt gttgaactta
     2041 tttttcctaa tgtttgtaga taatccatac atttttttcc taacatattt acctcctaaa
     2101 aatataattt ttatttaagc taagattagc ttttatactt ataatttttg aactttcaaa
     2161 taattttgta tgtatagtaa aatactattt tagtattaat aatgatataa gaacataaag
     2221 tctgtagacg aagagcagaa gttttaagtt aaaaaatatt aaatgtgatt tttattatta
     2281 tagtattttt ctaaaataat atctctttcc agttagagga taattttcaa gtgaaaatac
     2341 tttatggtat ccaaatttct cataaaattt aggtgcttga aaactgaatg tatctaaaaa
     2401 aacatattta caacctcgtt ctttagcaat tttttcagcc ttatataaaa gttgacttcc
     2461 agtaccttta tatctaagct tctcactaac ccataagtat tttatagaaa gccaattacc
     2521 atgagtaaca cctataaaac ctgcaacaat tttacctgct ttatctcgaa tatatattcc
     2581 taaatccact ggatttttat cttcaagtct agcaagatta tattttaata atccttgaaa
     2641 aatagtatct tcatcttgtt tttctatttt ttctgtaatt tctaattcca tatttgaact
     2701 ttctccttgt ataatctttt gcatattggt attttttaat aaaatcagta gaaagaaaat
     2761 agtttaacat tattctaata gtcatttatg ctaatgtcaa actaataata cttattctta
     2821 attttatatt gttgatatta aatttttaaa aataaaaatg tagtttaagt tatataaaaa
     2881 aatcatcaat aaattcagta aacttaaatt gttgaatatt aaaataagtt aatttaatac
     2941 ataaaaacta ttttagatgt tattctattg ctatgttcaa taatatcatt agtagctcaa
     3001 tttataaaat gactaaatta tataaataaa gttagaaagt agtgtatagt tttattaaat
     3061 ataagtaata aaataataaa tacctagtag aaattaataa ttgaaaaata aaatactacc
     3121 aaaataaaat cgatagtatt t
//
LOCUS       DAFNNB010000232         3128 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0238, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000232 DAFNNB010000000
VERSION     DAFNNB010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0238"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(73..168)
                     /locus_tag="L3M60_003401"
                     /pseudo
     CDS             complement(73..168)
                     /locus_tag="L3M60_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WxcM-like domain-containing protein"
     gene            complement(262..1206)
                     /locus_tag="L3M60_003402"
     CDS             complement(262..1206)
                     /locus_tag="L3M60_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HBN6228022.1"
                     /translation="MKLITHEQIMSLNISQKQGYEWIKESFLTKKDCQLPPKISMVED
                     GHVFYNVMPSLNHNHMIGGIKMVTRLPGRNPSLNSEIFLYNMEDAKLKAILDGNYITA
                     FRTAAVAALAIDTFAKKDFNTIAIMGLGVTASAIMNMLSYTLKDKNINIKLLKYKDQA
                     ESFIKKYEKNEQFSFEICDTHQELIVDSDVVISTVTYTDEYLSEYKWFKKGALLLPVH
                     VMGFGNVDYLFDKVYLDDYNHLKHLENIDRYKYSCEFYDVLSKQGKGRENDDERIISY
                     NIGLSIHDILFANKIYELCEILNCEEIELKEPKEKYWI"
     gene            complement(1226..2458)
                     /locus_tag="L3M60_003403"
     CDS             complement(1226..2458)
                     /locus_tag="L3M60_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HBN6228023.1"
                     /translation="MKRLMILGSIKYFENIVMSAKKEGIYTIVCDNRIDTPAKKICDE
                     AIDVDVFDFDKIKSIAIDKKIDGILTGFTDSLMKPYVYVANELNLPCVISCNQLESVT
                     NKAVMKKYFKNNGIPTTDYCIVESLKDLEKIQELNFPLVIKPVDGYGSRGVYFVSNIE
                     ELIEKFEYSKEVSQDGQVIIEEFYESEEIQGLAWVHNGESHVFYIGDRELVNIHTGRA
                     GKPDRLIYPSKYCFQYEEEIKSIYQNITQAFDIKNGPLYVQMLVGAYGIKVSEVMPRL
                     PGGCDYMAISQITDLDIGSLFANFSVNNEINFDEIKKYNMHLSKCVYALPIYIKPGTI
                     KKINNVDKIESLDYVTQILLLVKEGDTIGLYEDERQDCGRVYGIANNIFDANIKKKCI
                     HEMIEILDENNENMIENF"
     gene            complement(2550..>3128)
                     /locus_tag="L3M60_003404"
     CDS             complement(2550..>3128)
                     /locus_tag="L3M60_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HBN6228024.1"
                     /translation="KNNYMFYDELSMFLELSKYKEDIVANFRYSKRRDFKYSLKNNLT
                     FKRLYSDEDIVCFFNLLSNCLDSKGAKVIHSIEELLDFKNNRLKDIVEFYAVYIDGIM
                     ICGSMVFNFQERVYHTQYLASNSTYNKYYPMIFLYYNLIKNALERNFKYLSFGISTLN
                     RGKYLDEGLATFKEGFGSSAICNRTYYKNINK"
BASE COUNT         1055 a          534 c          275 g         1264 t
ORIGIN      
        1 gtttccctat atgctgacta ttttagctac taactcataa ctaaagtcac aagtgtgcgt
       61 agctattttt taatcaaaag gtattacttt atttgattct aatgcaacta aatttcctat
      121 atattcattt ccaatagata cgaacttaat taaattatat ttatccataa atttatctat
      181 cctattcata ctaattttct aacaatatat tacttcatat tcatatttaa aataaattac
      241 cataaatatg aatgaaataa actatatcca atatttttct tttggttctt ttaattctat
      301 ctcttcacaa tttagtattt cacacaattc gtaaatttta tttgcaaata atatatcatg
      361 tattgacaaa cctatattgt aagatattat tctctcatca tcattttctc ttcctttacc
      421 ttgtttactt aaaacatcat aaaattcaca agaatactta tatctatcta tattttctag
      481 gtgtttcaaa tgattataat catctaaata taccttatca aatagataat caacatttcc
      541 aaatcccata acatgtacag gtaataataa agcacctttt ttaaaccatt tatattctga
      601 taaatattca tctgtatatg tcacagtaga aataactaca tctgaatcta caataagctc
      661 ttgatgtgta tcacatattt caaaactaaa ttgttcattt ttttcatact tttttataaa
      721 tgactctgct tggtctttat atttaagtaa ctttatattt atatttttat ctttaagagt
      781 atacgaaagc atattcatta tagcagaagc agttacacct aaacccataa tagcaatagt
      841 attaaaatct tttttagcaa atgtatcaat agccaaggca gcaacagcag cagtcctaaa
      901 agctgtaata taattaccat ccaaaatagc ctttaattta gcatcttcca tattatatag
      961 aaatatctct gaatttaaac ttggatttct ccctggcaac cttgtaacca tttttattcc
     1021 accaatcata tgattatgat ttagggaagg cattacattg taaaatacat gtccatcttc
     1081 taccatgctt atttttggtg gtaattgaca atcttttttt gttaaaaagg attcttttat
     1141 ccactcatac ccttgtttct gagaaatgtt taatgacata atttgttcat gagttatcag
     1201 cttcatatct atcatactcc cttatttaaa aattttctat catattctca ttattttcat
     1261 caagtatttc tatcatttca tgaatacatt tcttctttat atttgcatca aatatattat
     1321 ttgctattcc ataaactctt ccacaatcct gtctctcatc ttcatataat cctattgtat
     1381 ctccttcttt tactagtaat agaatttgag tgacataatc taagctttct attttatcta
     1441 cattatttat cttttttatt gtcccaggtt ttatgtatat tggtaatgca tatacacatt
     1501 ttgatagatg catattatat ttctttattt catcaaaatt tatttcatta ttcacactaa
     1561 agtttgcaaa taagcttcct atgtctaaat ctgttatttg agaaatagcc atatagtcac
     1621 aacctccagg taatcttggc attacttcag atacctttat tccatatgct cccactaaca
     1681 tttgtacata aagaggtcca ttttttatat caaaagcctg tgttatattt tgatatatag
     1741 attttatttc ttcctcatat tgaaaacagt actttgatgg ataaattaat ctatctggtt
     1801 ttccagctct tcctgtatgt atatttacaa gctctctatc tccaatataa aatacatgag
     1861 actctccatt atgaacccaa gctagacctt gtatttcctc actttcataa aattcctcaa
     1921 ttatgacttg tccatcttgt gaaacttcct tagaatattc aaacttttct attaattctt
     1981 ctatatttga tacaaaatat acaccccttg aaccatagcc atcaacaggt tttattacga
     2041 gtggaaaatt taattcttgt attttttcta aatcttttag actttcaaca atacaataat
     2101 cagttgtagg tattccattg tttttaaaat attttttcat aacagcctta ttggttacag
     2161 attcaagttg attgcaagaa attacacatg gtaaatttag ttcatttgca acataaacat
     2221 atggtttcat taaagaatct gtaaatccag ttaaaattcc atctatcttt ttatctatag
     2281 ctatactttt tattttatca aaatcaaata catcaacatc tattgcttca tcacaaatct
     2341 ttttagcagg agtatcaatt ctattatcac aaacaattgt gtatattcct tcttttttag
     2401 ctgacataac aatattctca aaatacttaa ttgagcctaa aatcattaat cttttcatat
     2461 ataaaccttc tttataaact aattattttt atctaagatt agagtactat atatttacta
     2521 tactctaaaa actctctaaa acattattat cacttattta tatttttata gtacgttctg
     2581 ttgcaaattg cacttgagcc aaaaccttct ttaaatgtag ctaaaccctc gtctaagtat
     2641 ttacctctat ttagagtgct aattccaaaa gaaagatact taaaatttct ctctaaagca
     2701 ttttttatta gattataata taaaaatatc attggataat atttattata tgtcgaattt
     2761 gatgctagat attgagtatg ataaactctt tcttgaaagt taaataccat actcccacaa
     2821 atcataatgc catcaatata tactgcataa aattctacaa tatcttttaa cctattattt
     2881 ttaaagtcca gaagttcttc tattgaatgt attactttag ctcctttact atctaaacaa
     2941 ttagaaagta aattaaaaaa acaaactata tcttcatcac tatacagtct tttaaaggta
     3001 agattgttct tcaaagaata tttaaaatct cttcttttac tatatctaaa attagctact
     3061 atatcttctt tatacttact taattctaaa aacatactta attcatcata aaacatgtaa
     3121 ttattctt
//
LOCUS       DAFNNB010000233         3035 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0239, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000233 DAFNNB010000000
VERSION     DAFNNB010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0239"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..849
                     /locus_tag="L3M60_003405"
     CDS             <1..849
                     /locus_tag="L3M60_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HBN6228025.1"
                     /translation="TPKLSEYIADINPDLIISTFPLAAACVNNFKIKNPDINIPTLTV
                     ITDVVDSMEWVFENTDLYFVPSPEIKNRFFQKGINPDSIKVTGVPVDKRFQIESKEIC
                     CDKYRLLLLGGGRGLFDIDEDFMHWIDEFIEEHSDSIEITIVTGKNKKLYDNLTHKKP
                     LKNIKVLGFVNDMYNLIRECDLMLTKPGGATIFEAIQSQTPVLVKMPKVGQEIENAKF
                     IIDKGLGMIYSDDLDLKNIFYRLVSNEFDSIINFMKKNLEEFKTVIHPEKIADYISEL
                     IDKHYS"
     gene            complement(977..2875)
                     /locus_tag="L3M60_003406"
     CDS             complement(977..2875)
                     /locus_tag="L3M60_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HBN6228026.1"
                     /translation="MDSLLYVIEKDKHTNEELREILKSNKNIRFVSLMGVDLGGNATD
                     EKIPVELFLDDIDKFLESAIQTDGSSVELYNIATLNNAKVDLMPDKSCHWYVDYNMEY
                     IDEEVGLPVGTLKIPAFLIHDNKKVCSRGVLQKADKYFKKSMYEIFREYPHVINNIGI
                     DSVDDIEEIMLTAATELEFWVNTPEDKADLEKLYVSQSLKEQYWKRTHGIIRTCLEKS
                     LIILQKLGVSPEMAHKEVGGIQSSISIDGRTNHAMEQLEVSWKFSTPLQAADNELLVR
                     DVIEDVFTSHGLEVTFKAKPIHGVAGSGGHTHVGVSAKLKDGSIKNLFAPKDLKEDYL
                     SELGYGALMGLLYNYEVLNPIVTASNDGFNRLVPGFEAPVCIVTSLGHSYEIPSRNRS
                     VLVGLIRDMKNPKTVRFELRSPSPLSNTYLVIAGCYQTMLDGIKAAAKSGLSTKELEK
                     ELSKNVGEESFYLEKDRAYRDENDVFEHYSLEERNARFGIPPATVYENMKNLEIYASK
                     LKSLKQGDVFTDSIIESFKIGAIDKWQKKLKTRIIEEGIQKIRSIVKIHTKENMDALD
                     EVVWNSISDLKFNIMKDTLTRESLFTRVREAVENKDYQAASDLQIELKRSMEEIQQLY
                     MQYKKNIY"
BASE COUNT          975 a          517 c          375 g         1168 t
ORIGIN      
        1 acacctaaac tttctgagta tattgctgat ataaatccag atttgattat ctctacattt
       61 ccactagctg ctgcttgtgt aaataacttt aagataaaaa atcctgatat aaatatacct
      121 acacttacag ttataacaga tgttgtagat agtatggaat gggtttttga aaatacagac
      181 ttgtattttg ttccttctcc tgaaataaaa aatagatttt ttcagaaagg aataaatcca
      241 gattccataa aggttactgg agttcctgta gataagagat ttcaaattga aagtaaagaa
      301 atatgttgtg ataaatatag actattgctt ctaggtggag gaagaggtct atttgatata
      361 gatgaggact ttatgcattg gatagatgaa tttattgaag aacatagtga ttctatagaa
      421 attacaatag ttactggtaa aaacaaaaaa ttatacgaca atctaactca taaaaaacct
      481 ctaaaaaata ttaaagtact tggatttgta aacgacatgt ataatctaat aagagagtgt
      541 gacctaatgc taactaagcc tggaggagca actatatttg aagctattca atcacaaact
      601 ccagtacttg taaaaatgcc aaaagttgga caagaaattg aaaatgctaa atttataata
      661 gataaggggc ttggcatgat ttatagtgat gacctagacc taaaaaacat attttatagg
      721 ttagtatcaa atgaatttga ttctataata aactttatga aaaaaaattt agaggaattt
      781 aaaacagtca ttcatccaga gaaaattgct gattatatat cagaattaat agataaacat
      841 tacagttaaa ttgtgctatt acaaaatttt aaacaaaaaa ttaaaggcaa tcaagtatgc
      901 ttttacactt gagaatttac tcatagtgct taataataca tgattgcctt tatcatatca
      961 aaatcaaatt gttatcttag tatatatttt tcttgtactg catatatagt tgttgtattt
     1021 cttccatact tctctttaac tctatttgta aatctgatgc agcctgataa tctttatttt
     1081 ctacagcttc tctcactcta gtaaatagcg actctctagt taaagtatcc ttcataatat
     1141 tgaactttaa atctgatatt gagttccaaa ctacttcatc aagtgcatcc atattttctt
     1201 ttgtgtgtat cttgacaata cttcttattt tttgaatccc ttcttctatt attctagttt
     1261 ttaatttctt ttgccactta tctatagctc caatcttaaa ggattctatt atactatcag
     1321 taaatacatc accttgtttt aaagatttta gttttgaagc atatatttct aagtttttca
     1381 tattttcata aactgtagct ggtgggattc caaatcttgc atttctttct tccaaactgt
     1441 aatgttcaaa tacatcattt tcatctctat atgctctatc tttttctaaa tagaaacttt
     1501 cttcacctac atttttagat aattcttttt caagttcttt tgtactaaga ccactcttag
     1561 ctgctgcttt tataccatcc aacattgttt gataacaccc tgctattact aaatatgtgt
     1621 tagatagagg gcttggtgat ctcaactcaa atcttacagt ctttggattt ttcatatctc
     1681 ttattaatcc aacaagcact gacctatttc ttgatggaat ctcataacta tgacctaaag
     1741 atgtaactat acatacagga gcttcaaatc caggaactaa tctattaaat ccatcattag
     1801 aagctgttac aattgggttt aaaacttcat agttgtaaag aagtcccata agagccccat
     1861 atcccagttc acttagataa tcttctttta aatcttttgg agcaaataga ttttttatac
     1921 ttccatcctt taacttcgca cttactccta catgtgtgtg tccaccactt cctgctactc
     1981 catgaattgg ttttgcttta aatgtaactt ctagtccatg tgatgtaaat acatcttcta
     2041 taacatctct tactaataac tcattatctg ctgcttgtaa tggtgtagaa aacttccaag
     2101 aaacctctaa ttgctccata gcatggtttg ttcttccatc tatactaata gaactttgta
     2161 ttcctccaac ctctttgtga gccatttcag ggcttacacc tagtttttga agtataatta
     2221 aacttttctc taaacaagtt cttataatac catgagttct tttccagtat tgctctttta
     2281 ggctttgtga tacatataat ttctctaggt cagctttatc ttcaggagta ttaacccaga
     2341 actcaagctc tgtagctgct gttaacatta tttcctctat atcatctact gaatctattc
     2401 ctatgttatt tattacatgt ggatattctc taaatatttc atacattgat tttttaaaat
     2461 acttatctgc tttttgaagt actcctctag aacatacttt tttattatca tgtattaaaa
     2521 atgcaggtat ctttaatgtt ccaacaggta gacctacttc ttcgtctata tattccatat
     2581 tataatctac ataccaatga caacttttat ccggcattaa atctactttt gcattattta
     2641 atgtagctat gttataaagc tcaacacttg aaccatcagt ctgtatagct gattccaaaa
     2701 acttatctat gtcatccaaa aataattcta caggaatctt ttcatctgtt gcattgcctc
     2761 ctaaatcaac ccccattaag gaaacaaatc ttatattttt attgcttttt aggatttctc
     2821 ttaattcttc attagtatgt ttatctttct ctattacgta taatagacta tccatttttc
     2881 cactctccca taatttttat gtaaatttta ttttgttaaa tacaagataa atttcatttt
     2941 ccttacattt atgaattttt cttgcaaatt attcgtgttt tattcgtgtt tttatttata
     3001 ttattatata tcaagtacaa aaagttcgca atatt
//
LOCUS       DAFNNB010000234         2990 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0240, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000234 DAFNNB010000000
VERSION     DAFNNB010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0240"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            186..554
                     /locus_tag="L3M60_003407"
     CDS             186..554
                     /locus_tag="L3M60_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN6228027.1"
                     /translation="MENLVKIFKALSDETRLNILILVSKRNICQKGISKYLGISDSAV
                     SQHIKILKDVGIITGYKEGYYVLYHINKESFNICVDFINSMLSNSSESFIDVFDVNTI
                     HLGCSKECKSIRKCCKRREK"
     gene            557..928
                     /locus_tag="L3M60_003408"
     CDS             557..928
                     /locus_tag="L3M60_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HBN6228028.1"
                     /translation="MKVCIPVEENKGLDSKPYGHFGSAPIFVVCDLESGEVKSLDNGD
                     LDHEHGKCQPLKALSGTTVDAVIVGGIGQGAIIKLNGMGIKVYRAEGDTISANLDLLK
                     NGKLVEFPSDHTCSHDGCGHH"
     gene            complement(1072..1527)
                     /locus_tag="L3M60_003409"
     CDS             complement(1072..1527)
                     /locus_tag="L3M60_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228029.1"
                     /translation="MNINTVQGDSIEVLLRQLGATKISKVSSTLYFIKFDLGDGWEIS
                     YTYNINAKDQYFLQRIEPYPIGRGLFNDEYEIVSFISKDLKKFLNAKNSSNFKTFVEV
                     TRKVNSIIDNVEELFLNYNVDGDDLKTLNKELNDILNDIDYIEKHTKKI"
     gene            complement(1566..2831)
                     /locus_tag="L3M60_003410"
     CDS             complement(1566..2831)
                     /locus_tag="L3M60_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HBN6228030.1"
                     /translation="MEFQQIIAICIFLIVMAAIITEKVNRSVAAVGGALLMIIFNILT
                     LDEGLSHIDFNTIGVLVGMMLFVAVVKNSGLFEYIAIWTAKKAKGDPWKIMICFAIIT
                     AILSAVLDNVTTVLLIGPMTIVITQILGLNPVPFLITQILASNIGGTATLIGDPPNIM
                     IGSAANLSFMDFVINLGPAVIVILAITIICFRFIYGKELVVNEKAKNAILKLDEKKSV
                     KDKPLLIKSLILIAFILFGFMFHSTIHIDSSVVALTGASIMLLIGKQDVDEIMAGIEW
                     STILFFMGLFVVVGGLVEVGIINKLAQALIGLTEGHLVFTMLLILWLSAIVSSFLDNI
                     PFVATLIPLILTMQAEGIDVMPLWWATSLGACLGGNGTLIGASANVVLAGIGNKHGHP
                     ISFKEYFKIGFPLMIISIIISTVYLIIKF"
BASE COUNT         1113 a          410 c          410 g         1057 t
ORIGIN      
        1 aaattactat taaaagtatt ttaaaatcta tatgtaatta cttttattaa aaaaaattaa
       61 aagttatatt agaaagaatt agaattattt ataatatata ccaaaattta aaaataatgg
      121 ttgaaaaatc taattaagag tactataatt taattaagta attacttaat caaaacgagg
      181 tacttatgga aaacttggtt aaaatattta aagcattgag tgatgaaact agacttaaca
      241 tacttatatt agtatccaag agaaatatat gtcaaaaggg gatttctaaa tatctaggga
      301 tttcagattc tgctgtttca caacatataa aaatattaaa agatgttggg ataataacag
      361 gatataaaga aggatactat gttttatatc atattaataa agaatcattt aatatatgtg
      421 tagattttat aaattctatg ttaagtaatt ctagtgaaag ctttatagat gtatttgatg
      481 taaatacaat acatctagga tgcagtaaag aatgtaaatc tataagaaaa tgttgtaaga
      541 gaagggagaa ataataatga aagtatgtat accagtagaa gagaataagg gattggatag
      601 caaaccatat gggcattttg gttctgctcc aatttttgtt gtgtgtgatt tagaaagtgg
      661 agaagttaag tctttagata atggcgattt agatcatgaa catggaaaat gccaaccatt
      721 aaaagcattg tcaggtacaa ctgtagatgc agtaatagtt ggaggaatag gacaaggagc
      781 tataattaaa ttaaatggaa tgggaataaa agtttacaga gccgaaggcg atactataag
      841 tgctaattta gacctactta aaaatggaaa attagtagag tttccatcag accatacttg
      901 ttcacatgat ggatgtggac atcattaaaa aaaatagctg atgaaacata tgcttcatca
      961 gctatttttt cttttaataa atacgatttt attcttttaa aaccaaaatt tagattttta
     1021 tgttttttgt aatatcctcg ataacatctc gagtttataa gtttactact tttagatttt
     1081 tttcgtatgt ttttctatat agtcaatatc atttaatatg tcattaagct ctttattaag
     1141 tgtttttaaa tcatcaccat ctacattgta gtttaaaaat agttcttcta cattatcaat
     1201 gatagaatta acttttctag taacttcaac aaatgtttta aagttactac tatttttagc
     1261 atttagaaat ttttttaaat cctttgatat aaatgatact atttcatatt cgtcattaaa
     1321 tagacctcta ccaataggat atggttcaat tctctgtaaa aaatattggt ctttagcatt
     1381 aatattataa gtataagata tttcccaacc gtctcctaaa tcaaacttaa taaaatataa
     1441 tgtactactt acttttgata tttttgtagc accaagttgt cttaaaagaa cttcaatcga
     1501 atcaccttga acagtattaa tattcatata tacacatccc ctttagtatt attatactat
     1561 attttttaaa attttataat taaataaaca gtagatataa taatagatat aatcataagt
     1621 ggaaaaccta ttttgaaata ctctttaaaa gagataggat gcccgtgttt attacctata
     1681 cctgctagta caacattagc agatgctcct attagtgttc catttcctcc aagacaagct
     1741 ccaagagatg ttgcccacca tagtggcatt acatcaatac cttcagcttg catagttaaa
     1801 ataagtggaa ttagtgttgc aacaaatgga atattatcta agaaagaaga gactatcgct
     1861 gataaccata gtatcaacaa cattgtaaat acaagatgtc cttctgtaag tccaatcaat
     1921 gcctgagcca atttatttat tataccaact tctactaatc caccaactac tacaaataat
     1981 cccataaaga ataaaattgt agaccattca atacctgcca taatttcatc tacatcctgt
     2041 tttcctatta gaagcattat agatgctcct gtcagtgcta caacagaaga gtctatatgg
     2101 attgtactgt gaaacataaa accaaaaagt atgaatgcta ttaatataag actttttata
     2161 agtagaggtt tatctttaac agattttttt tcatcgagtt tcaatattgc gtttttagct
     2221 ttttcattta caacaagttc ttttccataa ataaatctaa agcatattat agttatagca
     2281 agtattacta taacagcagg acctaaattt attacaaaat ccataaaact aagattggca
     2341 gcactaccaa tcataatatt tggagggtct cctataagag tagctgttcc tcctatattt
     2401 gaagctaaaa tctgtgttat taaaaatgga acaggattaa gtcctaatat ttgtgtaata
     2461 actatagtca ttggtccaat aagtaagaca gttgtaacat tatctaatac agctgataaa
     2521 atagctgtta taatagcaaa acaaatcatt atcttccatg ggtcaccttt agctttcttt
     2581 gcagtccaga tagctatgta ttcaaataat cctgagtttt tgacaacagc tacaaatagc
     2641 atcataccaa ctaatacacc aatagtgttg aaatctatat ggcttaatcc ttcatcaagc
     2701 gtaagtatat tgaatattat cattaatagt gcaccaccaa cagctgctac ggatctgttt
     2761 accttttctg taataatagc tgccataact attaaaaata tacatatagc tattatttgt
     2821 tgaaattcca taatataagt acctccattt gtattatttt gagcctaatg ctagtactat
     2881 atatagtatt acttttttat tgttttttca atggatgtat gaaagtatat tatttaacaa
     2941 agtttttgaa aaattgacag aatttaaaga aaatttttgt tatttaaata
//
LOCUS       DAFNNB010000235         2947 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0241, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000235 DAFNNB010000000
VERSION     DAFNNB010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2947
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0241"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            335..1753
                     /locus_tag="L3M60_003411"
     CDS             335..1753
                     /locus_tag="L3M60_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBN6228031.1"
                     /translation="MTELMIISGLVLLICITSSKVLYKFGVPILLIFILLGMLFGSDG
                     MVGIYFDNYELTKQLCSIGLIFIMFFGGFGTNWNMAKPVAIPSILMSSLGVIITAGVT
                     GIFCHMVIGTSLLEGLLIGAIAASTDAASVFAVLRSQKLNLKGSLASLLEVESGSNDP
                     IAYMLTLILLTLMNNSGVELIVPMVIKQIVFGLAIGFVLAKLSVYILRHSNFEIEGFY
                     TIFITAIAILSYAFSEYIGGNGYLSVYISGIIIGNSKIPHKKSLVHFFDGISWIMQIM
                     LFFMLGLLSFPSKLPNIFGIAMVISIFMIVIARPLATFIVLAKFKFSNKEKLFISWVG
                     LRGAASVVFAIYAVTQGVIIENDIFHIIFFIALLSVGVQGTLIPAVARKLDLVDDNTP
                     VLKTFNDYDGEISSKLIEVNVDDSSKWANKSIMDSNIPEDILIVMIKRKEQVLVPKGS
                     TVIKTGDTLVLSGDRIEELIQL"
     gene            1829..2641
                     /locus_tag="L3M60_003412"
     CDS             1829..2641
                     /locus_tag="L3M60_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HBN6228032.1"
                     /translation="MFSIGMFSKINKITTKTLRYYEDIGLLKPEYVDEFTKYRYYTTE
                     QLPKLHQIITLKQMGLSLAEIKEAIENPEEVENILKNKEKEMLDVIKSEEYKLIKLRN
                     YLVNLKGEVNMNDVIIKSLPKVKIASMRKVLKGYDELFNLFPNVMAEEMMKSECTCAV
                     PDYCFNIYHDGEYRETNMDVEMCQAINDLKEDTDILKFKELDEVESAVCILHNGSYST
                     LGKTYADAFKWIEDNKYKVVGNPRESYIDGIWNKESEEDWLTEVQIPVKRIL"
BASE COUNT         1110 a          296 c          509 g         1032 t
ORIGIN      
        1 tacctaacat tacatgttag tttcttgtta cttatctaat taatttatat ttcatagtta
       61 tttaaaatta ctataaaaag gtgaaatata ttaaacaatc aactgaaaaa tgaaatatta
      121 ataaaaaaaa attcaaatta taactattaa atgaatataa ttaaaataaa tatataaatt
      181 taagtataac actcatattt taatgtattg tgaaattttt attgtacttt atacactata
      241 acttataaaa cctatgtcat attatatttg aataagtaat aaaattgtag atgtaaatta
      301 atctacaaaa tttatatgaa atggagttga tgctatgaca gaattaatga ttataagtgg
      361 gttagtacta ttgatttgta taacttctag caaggtattg tataaatttg gagtaccaat
      421 attattgatt tttatcttac tagggatgtt atttgggtct gatggaatgg taggaatcta
      481 ttttgataac tatgaattaa caaaacaatt atgctctatt ggactaatat tcataatgtt
      541 ttttggaggg tttggaacaa actggaatat ggctaaacct gttgctatac cttctatatt
      601 gatgtcttca ctgggtgtaa taattacggc tggggttaca ggcatatttt gccatatggt
      661 aataggaact agtttacttg aaggtttatt aattggggca attgcagctt caacagatgc
      721 tgcatctgtg tttgctgtgt tgcgttctca aaaattaaac ttaaagggtt cacttgcctc
      781 attattagaa gttgaaagtg gaagtaatga cccaatagcg tatatgttga cactaattct
      841 tttgacattg atgaacaatt ctggtgtaga gcttattgta ccaatggtaa taaaacaaat
      901 agtttttggg ttagctatag gatttgtact agcaaaatta tctgtttata ttttaaggca
      961 ttcaaacttc gagatagaag gattttatac tatatttata acagctattg caatactttc
     1021 gtatgcattt agtgagtata tcggaggaaa tggatattta agtgtgtata tttcaggtat
     1081 tattatagga aatagcaaga tacctcataa aaaaagtttg gttcatttct ttgatggaat
     1141 ttcatggatt atgcagataa tgttattctt tatgttagga ttgttatctt tcccttccaa
     1201 gctaccaaat atatttggta tagcaatggt catatctata ttcatgatag ttattgcaag
     1261 accattagca acatttatag tactagcaaa atttaaattt tcaaataaag aaaaactatt
     1321 catatcatgg gtaggtctta gaggtgcagc ctcagtagtt tttgcaattt atgcagttac
     1381 tcaaggtgta ataatagaga atgatatatt ccatataata ttttttatag ctttactttc
     1441 agtaggggta caaggtacat tgataccagc agtagctaga aagctagatt tagttgatga
     1501 taatacacct gtattaaaaa catttaatga ttatgatgga gaaattagct ctaaacttat
     1561 agaagtaaat gttgatgata gtagtaagtg ggcaaataag agtataatgg attctaatat
     1621 accagaagat atattaatag ttatgataaa acgaaaggaa caggttttag tgcctaaagg
     1681 ctcaactgtt ataaaaacag gggatacgtt ggtattaagt ggagatagaa ttgaagaatt
     1741 aatacaatta taatttattt cgtattgact ttaacctaag gggaaggtct aaacttaaat
     1801 taatcaaatc taatgcgcag gtgatgagat gttttctata ggaatgtttt ctaaaataaa
     1861 taaaataact acaaagacat taaggtatta tgaagatata gggttgttaa aaccagagta
     1921 tgtagatgag tttacaaaat ataggtacta tacgacagag caattaccta aattacatca
     1981 gataataact ttaaaacaaa tgggtttgtc attagctgag ataaaagaag ctatagaaaa
     2041 tcctgaagag gttgaaaata ttttaaagaa taaagaaaaa gaaatgttag atgtaattaa
     2101 aagtgaagaa tataaactca ttaaactgcg taattacctt gtgaatttga aaggagaagt
     2161 caatatgaat gatgtaatta ttaagtcttt accaaaagtt aaaattgcct ctatgagaaa
     2221 ggtattaaag gggtatgatg aactttttaa tctatttcca aatgtaatgg ctgaagaaat
     2281 gatgaagtca gagtgtacat gtgcagttcc agattattgt ttcaatatat atcatgatgg
     2341 tgaatacaga gagaccaata tggatgttga gatgtgtcaa gctataaatg atttaaaaga
     2401 agatacagat atattaaaat ttaaagaact agatgaagtt gaaagtgctg tttgtatatt
     2461 acacaatggt tcatattcaa cattgggaaa aacttacgca gatgcattta aatggataga
     2521 ggataataaa tataaagtgg ttggaaatcc cagagaatct tatatagatg gtatatggaa
     2581 taaagagagt gaagaagatt ggttgacaga agttcagata ccagtgaaac gcatattata
     2641 atatgtatac aaagtataaa taagaataaa aaagtggtgt ctcaaaatga attttttagt
     2701 ttatgaggca ccttttttca tatgaaatca agtatatttt tataatttca atgatgaaaa
     2761 aaagcttatc tctattttga gacagcctct tattttttta ttataatagt tttctaaaga
     2821 tgttctattt attaaatttt tataaataat tatatttaat atacaaaata tgcataaatc
     2881 ttgatgtttt atatataaag ttgtatattt tttaaggata taaagtaaaa agtgtaaagt
     2941 atcaaga
//
LOCUS       DAFNNB010000236         2927 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0242, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000236 DAFNNB010000000
VERSION     DAFNNB010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0242"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            94..2322
                     /locus_tag="L3M60_003413"
     CDS             94..2322
                     /locus_tag="L3M60_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HBN6228033.1"
                     /translation="MIHLKKTNSLKEILNWNNIKNLKPSKGLKNILEIFKSDLKDIRG
                     NYAVLVIVLGLAFLPSLYAWFNIKASWDPYGSTANMLVAVTNEDKGNEIAGQKINVGD
                     QLIEQLKDNKSLGWQFVDEKTALEGVKKGTYYASLKIPKNFTKDLTSLITNDVKKGEI
                     IYTVNEKINAVAPKITDKGASTIQLQVNQTIVKTVSEIVLGISNGVGAGIEHSLPKLT
                     KVENSLIDLQKRFDHINKTVDLASDATSKLSDTVKDIKSDLPTIKKTLNDTKLLSSDL
                     KKFLEDTNDNLDELSPLIKSDLNLMVDLSSSASSLTLNLIDAVNSGSEDVPKLIDNLS
                     EKLSNLQSLNDTLVDFLTKLNQLTSNNRLDDVINNLEDSSNKIDSSISTLNDIKNKVI
                     SGQQPSVSALNNVLSLSNGIGRINLNILNNFDSKISKPINSIFANSIKVANDIITVLD
                     KAESKLPKVEEILTTSLKLSGNAQESISLIRERLPLAKGMLDDLIETLNKISNGEDMK
                     KLVSLLESDILTKSNFLKEPVEVKTEKLYPIENYGSAMTPFYSVLSTWVGILLISALL
                     STSVHGNYKPYEIYFGRGLTFLSIALVQGFIIGTGDILLLGVTAKHPILLIILLMFTS
                     MVFNFIVYSLVSVFGNVGKAIAIVLLVVQIGSSGGTFPIQVTPTFFQRINPIMPFTYA
                     IGATREAVGGIYAPNLTKDILVLLLFMILSICLNVLLKGPINRIIKPFNDKFGDSNLT
                     GH"
     gene            complement(2558..2857)
                     /locus_tag="L3M60_003414"
     CDS             complement(2558..2857)
                     /locus_tag="L3M60_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBN6228034.1"
                     /translation="MYKNINKNQLKEMMKNEKDILLLDVRTKDEFREHKIENAINISL
                     QELINNIDEIYEYKDKKVVVYCRSGHRSVTACNLLAEEGFEHLYNLNSGIIDYMN"
BASE COUNT         1088 a          393 c          390 g         1056 t
ORIGIN      
        1 acaaaatttt aactttacta tttgtgtaaa aagtaatata atttaaaatt gattataaat
       61 ctaacttttt tctttatcaa aatattttag gaggtgatac atttgaaaaa aactaatagt
      121 ttaaaagaaa ttttaaactg gaataatatc aagaatttaa aaccttcaaa gggtttaaaa
      181 aatatattag aaatttttaa aagtgaccta aaagatatac gtggtaatta tgcagtactt
      241 gtaatagttt taggtcttgc ttttttacca tctctgtatg cttggttcaa tataaaggca
      301 tcttgggacc cttatggaag cactgctaat atgcttgtag cagtaacaaa tgaagataaa
      361 ggaaatgaaa tagcaggaca aaagattaat gtaggagacc aattaattga acaacttaag
      421 gataacaaaa gtttaggttg gcagtttgta gatgaaaaaa ctgctcttga aggtgttaag
      481 aagggtactt actatgcatc acttaagatt ccaaaaaatt ttactaaaga tttgacatct
      541 ttaattacaa atgatgttaa aaaaggcgaa attatctata ctgttaatga aaaaataaat
      601 gcagttgcac caaagattac agataaaggt gcttctacaa tacaattaca ggttaatcag
      661 actatagtaa aaacagttag tgaaatcgtt cttggtatat caaacggagt tggtgcagga
      721 atcgaacaca gtcttccaaa gttaacaaag gttgaaaatt cccttattga ccttcaaaag
      781 agatttgacc atataaataa gactgttgat ttagcttctg atgctacttc aaaattatct
      841 gatacagtta aagatattaa atctgatttg cctacaatta aaaagacttt gaatgataca
      901 aaactgttat caagtgactt aaagaaattc ttagaggata caaatgataa tttagatgaa
      961 ctttctcctc ttattaagtc tgacttaaat ttaatggttg acctttcgtc ttctgcttct
     1021 tctcttacat taaatttaat tgatgcagtt aatagtggca gtgaagatgt acctaagttg
     1081 atagataatt tatcagaaaa actttctaat ttacaatctt taaacgatac tttggtggat
     1141 tttttaacta aattaaatca acttacatca aataatagat tggatgatgt aataaataat
     1201 cttgaagact ctagcaataa aatagattca tctatatcaa cattaaatga tattaaaaac
     1261 aaagttatat ctggacaaca accatctgta agtgctttga ataatgtttt aagtctatca
     1321 aatggtattg gaagaattaa tttaaatatt ttaaataatt ttgactcaaa aatatcaaaa
     1381 cctataaata gtatatttgc aaacagtata aaagtagcaa atgatataat tactgtttta
     1441 gataaagcag aatcaaaact tccaaaggtt gaagaaatac ttactacatc tctaaaactt
     1501 tcaggaaatg cacaggaaag tatatcatta ataagagaaa gacttccttt agcaaaaggg
     1561 atgttggatg atttgattga aactttaaac aaaattagta atggtgaaga tatgaaaaaa
     1621 ttagtcagct tgcttgaaag tgatatattg acaaaatcta atttcttaaa agaacctgtt
     1681 gaagtgaaaa cagaaaaatt atatccaatt gaaaattatg gttctgcaat gacaccattt
     1741 tatagtgtcc tttctacatg ggttggtata ttacttattt cagcattact atctacatct
     1801 gtacatggaa actataaacc ttatgaaatt tactttggaa gaggtcttac ttttttaagt
     1861 atagctcttg ttcaaggttt tataatcgga actggagata tattattatt aggagtaaca
     1921 gcaaaacatc caattttatt gataattttg cttatgttta ctagtatggt atttaacttt
     1981 atagtgtatt ctttagtttc tgtatttgga aatgtaggaa aagcaattgc aatagtacta
     2041 ttagtcgttc agattggttc ttctggtggt acattcccta tacaagttac acctacattc
     2101 ttccaaagaa taaaccctat catgccattt acttatgcaa taggtgctac aagggaagca
     2161 gttggtggta tatatgctcc aaatctaact aaagatatac tagttctatt gctatttatg
     2221 attctgtcta tttgtttaaa tgtccttctt aaaggaccta taaatagaat tataaaacca
     2281 tttaatgata aatttggcga tagtaactta actggtcatt aaaaaattaa ttctgtattt
     2341 aaatatattt tttaacaata ctttaagttc atattaaaag gtagtagctt tttagctact
     2401 accttttttc aacttaaaaa ctaatattta ctacagaata tgcctcaaaa aaagtataaa
     2461 aattcgttct ttcctaaatc tatcttataa atatctaaag atattcctac ttaataagca
     2521 gaattatata tagtatttaa tgcataactt atattattta attcatgtaa tcaattattc
     2581 ctgagtttaa attatataaa tgttcaaaac cttcttcagc taacaaatta catgcagtaa
     2641 cacttctatg accacttctg caataaacca ctactttctt atctttatac tcgtaaattt
     2701 catctatatt attgattaac tcttgtaatg atatatttat agcattttct attttatgtt
     2761 ccctaaattc atctttagtt cttacatcca ataataaaat atccttttca tttttcatca
     2821 tttctttaag ttgattctta tttatattct tgtacatata tctaaaattc ctccattttt
     2881 atttatatat tacctatacc ctttattctt tatacagcaa caaatat
//
LOCUS       DAFNNB010000237         2925 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0243, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000237 DAFNNB010000000
VERSION     DAFNNB010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0243"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            108..884
                     /locus_tag="L3M60_003415"
     CDS             108..884
                     /locus_tag="L3M60_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HBN6228035.1"
                     /translation="MEYIIFDLEFNQGFDKKFNKTISNEKCPFEIIQIGAIKLDSKFN
                     IIDTFNSYVKPTIYKEIHPFISRMTNIKDSDFNDSPTFPEVYNNFIKFISSQDPILCV
                     WGAGDLKELYRNINYHKLPSNSLPKSYINIQQHASKYFNNPAGKSIGLQNAISILELD
                     EKMSYHNALNDAYYTAKVFIKIYNPSIVPDIYLYTSIKPKTIRYSNKKRVDYDKLFDE
                     FRKILNRELTKDEKKIINLAYNMGKTNQFILEDAKQRKNK"
     gene            complement(976..1728)
                     /gene="xth"
                     /locus_tag="L3M60_003416"
     CDS             complement(976..1728)
                     /gene="xth"
                     /locus_tag="L3M60_003416"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003453531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBN6228036.1"
                     /translation="MRFISWNVNGIRACAGKGFLDFFKEVDADVFCLQETKLQEGQIE
                     LDLPGYFQYWNYAERKGYSGTAIFTKKEPLKVMYGINIEEHDKEGRVITLEFEDFYFV
                     TVYTPNSQSELKRLEYRTRWEDDFIDYLTKLDNHKPVIVCGDMNVAHKEIDLKNPKNN
                     MKNAGFTKEEREKFSKLLDSGFIDTYRYFNPDKEGVYSWWSYRFNARKNNAGWRIDYF
                     CASKKLEDRLISADIHTEVLGSDHCPVELEIK"
     gene            complement(1849..2799)
                     /gene="galT"
                     /locus_tag="L3M60_003417"
     CDS             complement(1849..2799)
                     /gene="galT"
                     /locus_tag="L3M60_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBN6228037.1"
                     /translation="MKELRIDPITNDVVIFATDRLKRPLDTADIPNEDEETNEYDKEC
                     PFCKGNEAYATDALFEIEGEEGWLVKSVYNKFPIIDDMARDIYGVHEVMIESSKHNRS
                     FYNMSQKEFEDVFFMYRNRFRDLSKDDKVEYVSIFKNFLRKAGASLMHPHAQILSMSF
                     IPPEITNELLVSKEYYDSNKSSLYDDLIENEISLNRRVVYNGEAFLVIIPYATKYSGE
                     VRIIFKDKIKFGELNDNNIKELSTIFEKLFKKLYNINGYMPFNLCIHTHPTKIETKSY
                     FNVHMHIIPRKYNFGGFELGTNMYVSSMSPEELTKKLKID"
BASE COUNT         1026 a          464 c          307 g         1128 t
ORIGIN      
        1 aatatggggt atactaaaat aaaagtattt ttatttgaaa atttaatttt atttataata
       61 taatctatag taaaagaaat aataaaaaaa tttaggagat aaactttatg gaatacataa
      121 ttttcgattt agaatttaat caaggatttg ataaaaaatt caataaaact atatccaatg
      181 aaaagtgtcc ttttgaaatt atacaaatag gagcaattaa attggattct aaattcaata
      241 taatagatac atttaatagc tatgtaaagc ctacgattta taaagaaata catcctttta
      301 ttagtcgtat gactaatatt aaagattctg attttaatga ttcgcctact ttccctgaag
      361 tttacaacaa ttttataaaa tttatatcat cacaagaccc tatattatgt gtctggggag
      421 ctggagattt aaaggaatta tacaggaata tcaactacca caaactacca tcaaattcct
      481 tacctaaatc ttatattaat atacagcaac atgcatctaa atattttaat aatccagcag
      541 gaaaaagcat tggattacaa aatgctattt ctatattgga acttgatgaa aaaatgtctt
      601 atcacaatgc tttaaatgat gcatattata ctgctaaagt ttttattaag atatacaatc
      661 caagtatagt accagatata tatctttata caagtatcaa acctaaaact ataagatatt
      721 ccaataaaaa aagagttgat tatgataagt tatttgatga gtttaggaaa attttgaatc
      781 gtgaattgac taaagatgag aaaaaaatca ttaacttagc ttataacatg ggaaaaacaa
      841 atcaatttat attagaagat gctaaacaaa gaaaaaacaa ataaagttta cttaggttaa
      901 aaataggcat gtagattaaa taattagtta gctttaaatt tatatctaca gcccaatttt
      961 tatcctaatt aatctttatt ttatttctaa ctcaactgga caatggtctg aacctaatac
     1021 ttcagtgtgt atatctgcac ttatcaatct atcttctaat tttttggatg cacaaaaata
     1081 atctattctc catcctgcat tattttttct tgcattaaat ctatatgacc accaactata
     1141 gacaccttct ttatcaggat taaaatatct atatgtgtct ataaacccac tatctaataa
     1201 tttagaaaac ttctctcttt cttctttagt gaaacctgca tttttcatat tatttttagg
     1261 attttttaaa tctatttctt tatgtgcaac gttcatatct ccacacacaa ttacaggctt
     1321 atgattatct agttttgtta aatagtctat aaagtcgtct tcccatctag ttctatattc
     1381 tagtcttttt aattcagatt gagaatttgg tgtatacact gtaacaaagt aaaaatcttc
     1441 aaactctaga gttatcactc taccctcttt gtcatgttct tctatattta ttccatacat
     1501 tacttttaaa ggctcttttt ttgtaaatat agcagtacct gaataaccct ttctttcagc
     1561 ataattccaa tattgaaaat atcctggcaa atccaactct atttgtcctt cttgtaattt
     1621 agtttcttgt aagcaaaata catctgcatc gacttctttg aaaaagtcca aaaatccttt
     1681 cccagcacaa gctcttattc cattaacatt ccatgatata aatctcatac tatactcctc
     1741 atctaaacag taaatttctt gttattcttc atttgctttt attttaacaa taatatacta
     1801 taatttcatc taaaattact atttacttaa tacctaaaaa tatatgtttt aatcaatttt
     1861 caactttttt gttagctctt caggactcat tgaagaaaca tacatgtttg ttccaagttc
     1921 aaatcctcca aaattgtatt ttcttgggat tatgtgcata tgaacattaa aatacgattt
     1981 tgtttcaatc tttgttggat gagtgtgaat acataagtta aatggcatat atccattgat
     2041 attgtacaat ttcttaaaca acttttcaaa tattgtagaa agttccttta tattattatc
     2101 atttaactca ccaaacttta ttttatcttt aaatattatt cttacttctc cactatactt
     2161 ggttgcatat ggtataatca ctaaaaaagc ttctccatta taaacaactc ttctatttaa
     2221 acttatttca ttttctatta aatcatcata caaagagctt ttattgctgt cataatactc
     2281 ctttgaaact aacagttcat tcgtaatttc aggaggaata aaagacatag acaatatttg
     2341 agcatgtgga tgcatcaaag atgctcctgc ctttcttaag aagtttttga agatactaac
     2401 atactccact ttatcatctt tggacaaatc tctaaatcta tttctataca taaaaaaaac
     2461 atcttcaaat tctttttgac tcatgttata aaaactacga ttatgtttac tgctctctat
     2521 cattacttca tgaacaccat atatgtctct tgccatatca tcaattatag ggaacttatt
     2581 gtagacagat tttacaagcc atccttcttc accttctatt tcaaataatg catcagttgc
     2641 ataagcttca tttcctttgc aaaatggaca ttctttatcg tattcattag tttcttcatc
     2701 ttcatttggt atatctgctg tatctaaagg tctttttaac ctatcagtag caaatataac
     2761 aacatcattg gtaattggat ctattcttaa ttctttcata gttaacacct cttagttaat
     2821 agcctttaat ataattatat gatatttatc gtatctgtta aataaattta taatattttt
     2881 tataattttg catatttatt attatatatt ttgtctatag ctgaa
//
LOCUS       DAFNNB010000238         2901 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0244, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000238 DAFNNB010000000
VERSION     DAFNNB010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0244"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            144..413
                     /locus_tag="L3M60_003418"
     CDS             144..413
                     /locus_tag="L3M60_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HBN6228038.1"
                     /translation="MFESRCGVCCDSCTKKEQVNCTGCPTMEKPFWGGECKVKTCCES
                     KELNHCGECDTFPCDMLLNTGKDQGFDPMVNIEQCRKWLEETVSN"
     gene            complement(606..851)
                     /locus_tag="L3M60_003419"
     CDS             complement(606..851)
                     /locus_tag="L3M60_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pathogenicity locus"
                     /protein_id="HBN6228039.1"
                     /translation="MGELQKIPGVGKATEKSLIMLGYTTIKSLKDANPAQMYEKECLM
                     RGQHIDRCQLYVYRCAVYFVSTENPEPEKLKWWYWKD"
     gene            1269..1454
                     /locus_tag="L3M60_003420"
     CDS             1269..1454
                     /locus_tag="L3M60_003420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228040.1"
                     /translation="MFSKKNEGNEFSNEGKGISKKIIKFFKSTKGIALLAFIFGVFFG
                     NISSPACSEDHEEVISN"
     gene            1506..1787
                     /locus_tag="L3M60_003421"
     CDS             1506..1787
                     /locus_tag="L3M60_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBN6228041.1"
                     /translation="MSDAEPWFKMKDDEKKAIEAENQRKAEEAKKAEEEKKGYDTGIT
                     YDQLARTSDDYKYKKVKFEGKVIQVIEDGDEVQIRFAVSGNYDKVVLCS"
     gene            complement(2295..>2901)
                     /locus_tag="L3M60_003422"
     CDS             complement(2295..>2901)
                     /locus_tag="L3M60_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_105161403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBN6228042.1"
                     /translation="KHFYFNTDGIMQIGVFKGPDGFEYFAPANTDANNIEGQAIRYQN
                     RFLYLHDNIYYFGNNSKAATGWVTIDGRRYYFEPNTAIGANGYKIIDNKNFYFRNGLP
                     QIGVFKGPNGFEYFAPANTDANNIDGQAIRYQNRFLHLLGNIYYFGNNSKAVTGWQTI
                     NGNMYYFMPDTAMAAAGGLFEIDGVIYFFGVDGVKAPGIYG"
BASE COUNT         1028 a          370 c          419 g         1084 t
ORIGIN      
        1 tttttggaat agtttttata aatttaccag tactaataat tttatgttta acgtttagat
       61 gacttaaaat caattatttg cacttttgta gtcatctaaa gttattttat aatattttta
      121 aataatggaa aggatgtttg aatatgtttg aatcaagatg tggtgtctgt tgtgacagtt
      181 gtacaaaaaa agaacaagta aattgtactg gttgcccaac tatggaaaaa ccattttggg
      241 gtggagaatg taaggttaaa acttgctgtg aaagtaagga acttaatcat tgtggtgaat
      301 gtgatacttt cccttgtgat atgcttttaa atacgggaaa agaccaaggt tttgacccta
      361 tggtaaatat tgagcaatgc agaaaatggc ttgaagaaac agtttctaat taattgtttt
      421 agaatgtttt gttaagcaat ttatttaata ctttgttaga tagtatgttt agtcatttgt
      481 tgattggtat atttagccat acaaatagat ttacataaaa tttacgacta aaaaagagtt
      541 gagtattagt ttttattatt aaaacagata ttcaactctt ttttatatct aacttttttg
      601 attttttaat ctttccaata ccaccacttt aatttttctg gttctggatt ttcagttgat
      661 acaaaatata ctgcacatct gtatacatat aactgacatc tgtctatgtg ttgtccacgc
      721 attaaacatt ccttttcata catttgtgct gggtttgcat ctttaagtga tttaatagtt
      781 gtatatccta acatgattaa agatttttct gttgctttcc ctacccctgg aattttttgt
      841 agttctccca tacttcacct tctttctggt atattatttt tgtattatac ttagtaccag
      901 atattttttt attatagtta atatttaatt ttttattata tcaatttatt tatgctcttt
      961 catctatcta tattttacca cctctaaagt actgagtcat ttaattacat cataatatag
     1021 ttttatacaa ataaaatact ttatgtttca tttaatatat aaaattcacc ttcaagaaaa
     1081 ttatattata atctgacatt tttaccttat tttccaaaat atattgaatt ttcttgcttt
     1141 attttgtaaa attatgctta ggggaaatat attttaggaa aatatgaata tataattttt
     1201 agtcaactag ttattttaag tttttaaatt ttaaaataaa atatatctaa taaaagggaa
     1261 attgtattat gttttctaaa aaaaatgagg gtaacgaatt tagtaatgaa ggaaaaggaa
     1321 tctctaagaa aataattaaa ttctttaaga gcacaaaggg tattgctcta ctggcattta
     1381 tttttggtgt gttttttggc aatatatcct caccagcttg ttctgaagac catgaggagg
     1441 tcatttctaa ttaaacatca gttatagatt ctcaaaaaac agaaatagaa actttaaata
     1501 gcaaattgtc tgatgctgaa ccatggttca aaatgaaaga cgacgaaaag aaagctattg
     1561 aagctgaaaa tcaacgtaaa gctgaagaag ctaaaaaggc tgaagaagag aagaaaggat
     1621 atgatactgg tattacttat gaccaattag ctagaacatc tgatgattat aagtacaaaa
     1681 aggtaaaatt tgaaggtaag gttattcaag ttattgaaga tggtgatgag gtgcaaataa
     1741 gattcgctgt gtctggaaat tatgataagg ttgtactatg tagttaaaaa aaatcaataa
     1801 ctccttcaag agtgttagag gatgattaca taactataag aggtataagt gctggaacta
     1861 taacttatga atcaactatg ggtggaaaca taactatacc aggaatagct gtagagaaaa
     1921 ttaattaata ttgttttgta ttatagttaa tatttttgta ttatagtcaa tatgtttaaa
     1981 gatgttttta taattgcaaa taaacagtta caagactcta aattagtttt tgcttttagc
     2041 atattatcta tgttctagta actattaatt atttagtatt aatatttcca tatatgaatt
     2101 ttattataaa gtagtaaaat tatttgttta aaatttagat tatcgtcaag aataatagat
     2161 tattaaatga tagaaaaatt ttaactaaaa gtcatgtatt acaataacac atgactttta
     2221 attaaatatc aatatttatt atataaaaat aatttctgag tatcacatga ataaattttt
     2281 gtcaaacata tattctagcc atatattcca ggggctttta ctccatcaac accaaagaaa
     2341 tatataacac catcaatctc gaaaagtcca ccagctgcag ccatagcagt atcaggcata
     2401 aagtaataca tattaccatt aatagtttgc catccagtaa ctgcttttga attattacca
     2461 aagtaatata tattgccaag taaatgtagg aatctatttt gataacgtat agcttgaccg
     2521 tctatattgt tagcatccgt attagcaggt gcaaagtatt caaatccatt aggtccttta
     2581 aacactccta tctgaggtaa accatttcta aagtaaaagt ttttattatc aataatttta
     2641 taaccattcg cacctatagc tgtattaggc tcgaagtaat atctcctacc atcaatagtt
     2701 acccaaccag tagctgcttt tgaattatta ccaaagtaat atatattgtc atgtaaatat
     2761 aggaatctat tttgataacg tatagcttga ccttctatgt tgttagcatc cgtattagca
     2821 ggtgcaaagt attcaaatcc atcaggtcct ttaaacactc ctatctgcat aataccatca
     2881 gtattaaaat aaaaatgttt a
//
LOCUS       DAFNNB010000239         2867 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0245, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000239 DAFNNB010000000
VERSION     DAFNNB010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0245"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..203
                     /locus_tag="L3M60_003423"
     CDS             <1..203
                     /locus_tag="L3M60_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228043.1"
                     /translation="FNKYQKGEIVQLYETKFSCNRNCIVTYADEKELVLMYYDKEIKE
                     LIYKTLTKEDLIFNDYELKLLS"
     gene            392..1108
                     /locus_tag="L3M60_003424"
     CDS             392..1108
                     /locus_tag="L3M60_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HBN6228044.1"
                     /translation="MSKKSGCSESQVKKVIKSLENKELIKKENRTKLNSKEKDSNIYF
                     VLTAKISRAYGDLPVGHSETHPRACGDLPVGHVVTSNKTNINKTNLTNNIYSSVEQKE
                     EKTKDKEIYKRIVDYLNKTADKSFKSTTKKTISLIDARLKEGFTEEEFYKVIDNKVLN
                     WLDNDKMNAYLRPETLFGNKFESYLNETPKKSLKGNESVKGKTKTNNNKFANFNQTFD
                     KYSEDELDAIIKKSQSAKFK"
     gene            1133..1735
                     /locus_tag="L3M60_003425"
     CDS             1133..1735
                     /locus_tag="L3M60_003425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228045.1"
                     /translation="MEINSVRELRELFELFTVIKAEGNNVVSCNETVNELSNKELIKA
                     NENKFKKPWNDALLYEINALDIIKKHKHSDRKEKAYKTYLGLENADIEEIYKIHYGDK
                     ENSETVASCEEISSAKTDTISCNHDSVSKGKDTSDSECTCFICSKKIENQEDVFKIPF
                     LTRDYLSSAKEIKNIEICEKCLDGNSWSIIRRKQFKKMKK"
     gene            1781..2062
                     /locus_tag="L3M60_003426"
     CDS             1781..2062
                     /locus_tag="L3M60_003426"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228046.1"
                     /translation="MIKNNVLSYEEKIKVLKKHGYSDFTKEYVLKEFDKEHAFYKSFL
                     RAIEKEDDGKGVLIKKIGKGQHQFSKGFIKTHTISELLEEAKRIRKILE"
     gene            complement(2101..2445)
                     /locus_tag="L3M60_003427"
     CDS             complement(2101..2445)
                     /locus_tag="L3M60_003427"
                     /inference="COORDINATES: protein motif:HMM:NF022172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HBN6228047.1"
                     /translation="MVTNQDCIRDILLLIESNTTPISPVLQFDTILDNLTYDKSTIVH
                     HINLIYQNNLVDDVNYGDDTVLEVMQLSLKGYQYLDKIRDNTKWNKIKSSTSSFLNMS
                     LPIAIEYILGKF"
     gene            2534..2698
                     /locus_tag="L3M60_003428"
     CDS             2534..2698
                     /locus_tag="L3M60_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228048.1"
                     /translation="MQKRITEMSILEKVDLIGQKQKKLQEKYKNLSKGELFSIACNEV
                     KKIEEQEGKF"
     gene            2695..>2867
                     /locus_tag="L3M60_003429"
     CDS             2695..>2867
                     /locus_tag="L3M60_003429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228049.1"
                     /translation="MNIGSYTLVKKQLIEELEEGNKQKEEYLKECEKHIKGLEKSLEN
                     FARDALESEECII"
BASE COUNT         1272 a          276 c          496 g          823 t
ORIGIN      
        1 aatttaataa atatcaaaaa ggtgaaattg ttcaattata cgaaacaaaa ttcagttgca
       61 atagaaattg cattgttaca tatgcagatg aaaaagaatt agtattgatg tactacgaca
      121 aagaaataaa agaattaata tataaaactt taactaaaga agatttgata tttaatgact
      181 atgaacttaa gttactatct taataaataa tttataaata gaagggggtg agggattgga
      241 acttttaaaa gattcaagga aaaaagattg gttttgggta gaaaacgatt taattgatag
      301 agaagatttg actatttatg aaaaaatgat ttatatgaca ttagttaggc atttaaacga
      361 aaatagtttt tgctttccaa gtcacaaaac tatttctaaa aaaagtggat gtagtgaaag
      421 tcaagtcaag aaagttataa aaagcttaga aaataaagaa cttataaaaa aagaaaatag
      481 aaccaaatta aattctaaag aaaaagatag taatatttat tttgtattga ctgcaaaaat
      541 aagtagggca tatggtgacc tacccgtagg tcacagtgaa acccacccta gggcatgtgg
      601 tgacctaccc gtggggcatg tggtgactag taacaagact aatattaaca agactaattt
      661 aactaataat atatatagtt ctgtagagca aaaagaagaa aagacaaaag acaaagagat
      721 atataaaaga atcgttgatt acttaaataa aacagcagat aaatctttta agagtacaac
      781 aaagaaaact atatctttga ttgatgcaag actcaaagaa ggctttacag aagaagaatt
      841 ttacaaggtc atagataaca aagtgttaaa ttggctagat aatgataaaa tgaacgctta
      901 tttacgccct gagacgttgt ttggaaacaa attcgagtct tatttaaacg agacacctaa
      961 aaagtctcta aaaggcaatg agagcgtcaa aggaaaaaca aagacaaata ataataaatt
     1021 tgctaatttc aatcaaacat ttgataaata ttcagaagat gaacttgatg caattataaa
     1081 gaaaagtcaa agtgctaaat ttaaataaaa ttaaaaaagg tggggtaaaa aaatggaaat
     1141 taatagcgta agagagttaa gagaattgtt tgaacttttt acagttataa aagcggaagg
     1201 caacaatgtt gtttcttgca atgaaactgt aaacgagtta agtaacaaag aactaataaa
     1261 agcaaatgaa aacaaattta aaaaaccttg gaatgatgct ctattatatg aaataaatgc
     1321 tttagatata ataaaaaagc ataagcacag tgatagaaaa gaaaaggcat ataaaactta
     1381 tctaggatta gaaaatgcag atatagaaga aatttataaa attcattatg gagataaaga
     1441 aaattctgaa actgttgcaa gttgcgaaga aattagttca gcaaaaacgg atacaatatc
     1501 ttgcaatcat gacagtgttt caaaaggtaa agatactagt gattcagaat gtacttgttt
     1561 tatttgttct aaaaaaatag aaaatcaaga agatgtattt aaaatacctt ttttaacacg
     1621 agattatttg agttcagcaa aagaaataaa aaatattgaa atttgtgaga aatgtttgga
     1681 tggtaattcg tggagtatta taagaagaaa gcagtttaag aaaatgaaga aataaatatt
     1741 taaattagta tctttacatg tgaaaaagga gagtgaaaaa atgataaaaa ataatgtttt
     1801 aagttatgaa gaaaaaataa aagtattaaa aaaacatggt tatagtgatt ttaccaaaga
     1861 atatgtttta aaagaatttg acaaggaaca tgcattttac aaatcatttt taagagcaat
     1921 agaaaaagaa gatgatggaa aaggtgtgtt aattaaaaaa attggaaaag gtcagcatca
     1981 attttcaaaa ggttttataa aaacacacac tatcagtgag ttattggaag aagcaaaacg
     2041 tattagaaaa atattagaat aaggcttaga aatgtctata atctaaacct tatacttaaa
     2101 ttaaaattta cctaatatgt actctattgc gataggtagg gacatattta aaaaacttga
     2161 agtggaactt tttattttat tccatttagt gttatctcgt attttatcta aatattgata
     2221 accttttaaa gacaactgca tgacctctaa aacagtatca tcaccgtaat taacatcgtc
     2281 aacgagatta ttttgataaa ttaaatttat gtgatgaaca atagttgact tatcgtatgt
     2341 gagattatct agtatagtat caaattgtaa tacaggcgaa atgggagttg tattagattc
     2401 aataagtaat aaaatatctc ttatacaatc ttggttagta accatagtat cacctccttt
     2461 tgctttagta tagcacagga gaaatgagat gataataaat tttactgtag cagaatgaga
     2521 aggagcaaag caaatgcaaa agagaattac agaaatgagt atattagaaa aagttgattt
     2581 aattggacag aaacaaaaga aactacaaga aaaatataaa aatctaagta agggagaatt
     2641 gttttctatt gcttgtaatg aagttaaaaa aatagaagaa caggagggaa aattttgaat
     2701 ataggtagtt atacattagt taagaaacaa ctaattgaag agttggaaga aggaaataaa
     2761 caaaaagaag aatatttaaa agaatgtgaa aaacatataa aaggtttaga aaagagttta
     2821 gaaaattttg caagggatgc gttagagtca gaagaatgca taataga
//
LOCUS       DAFNNB010000240         2858 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0246, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000240 DAFNNB010000000
VERSION     DAFNNB010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0246"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            145..1026
                     /locus_tag="L3M60_003430"
     CDS             145..1026
                     /locus_tag="L3M60_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HBN6228050.1"
                     /translation="MVKKRKKKTVALTIIVVIVIGIFSMAWADMSRKKEMKETQERLK
                     AERLEKERIEKEKNEPIIGAKKEAIKEYGYDAKIVWDKLNKYDYSNNGEKIVFLTFDD
                     GPSTTNTPQVLDILKRHGVRGTFFIKGDSLERKGANEILKRTFDEGNAIAHHSYTHDY
                     KKLYPGRSLNLDTFVNELNKTDEAMKKVLGKNFSSNVVRCPGGYMSWKNMEPLGNYLK
                     EKNMASIDWNALNADAEGKKKNAQELFEHAKKSSEGKEMVVLLMHDTYGKQETVNALD
                     QIITYFKDNGYQFKILI"
     gene            complement(1087..2295)
                     /locus_tag="L3M60_003431"
     CDS             complement(1087..2295)
                     /locus_tag="L3M60_003431"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBN6228051.1"
                     /translation="MKSIDEQMRIIMKGVDDLIDEKELREKLIKSEKEGKPMIVKLGL
                     DPSAPDIHLGHTVVLRKMKQLQDLGHQIVIIIGDFTGKIGDPTGKSKARKALTTEQVL
                     ANAKTYEEQIFKVLDKEKTIVRFNSEWLAKLNFEDVIKLAATITVARMLEREDFKKRY
                     EGQMPISVHEFFYPLMQAYDSIALEADIELGGTDQRFNLLMGRSLQREFGMESQIVIM
                     MPLIEGLDGKEKMSKSLGNYIGIDEEAGIMYQKSMEIPDELIIKYYNLVTDVHPDEVN
                     KIESQLKDGSVNPRDIKMNLAREIVTLYHGEESAREAEERFKSVFQKGQIPEDIQTIQ
                     VKEDGFDLIEVLVVNEIVKSKSEVRRLASQGGVKVNGEKVEDLSTIVKESELVVQIGK
                     KKFVKIELVK"
     misc_binding    complement(2368..2620)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          945 a          473 c          345 g         1095 t
ORIGIN      
        1 actttaatat tatgcaaata atgtcatata agttacactt tagttatctt agttacaact
       61 ttgtcggttt actttttcta ctttcttcga tataattata atatattttt gatacaattc
      121 gacaaaaggg ggaatgctgt aattatggta aaaaaaagaa aaaagaaaac tgttgcactt
      181 actataattg ttgtaatagt tattggtatt ttttctatgg catgggcgga catgtctaga
      241 aaaaaagaaa tgaaagaaac tcaagaaaga cttaaagctg aaagattaga aaaagaaaga
      301 attgaaaaag aaaaaaatga acctattatt ggtgcaaaaa aagaagctat aaaagaatat
      361 ggatatgatg caaaaatagt ttgggacaaa cttaacaaat acgactattc aaacaacgga
      421 gaaaagatag tctttcttac atttgatgat ggaccatcta ctactaatac tccacaagtt
      481 ctagatatct taaaaagaca tggtgttaga ggaactttct ttattaaagg tgattctctt
      541 gaaagaaaag gcgctaatga aattttaaaa agaacatttg acgagggaaa tgcaatagcc
      601 catcactctt atactcatga ctataaaaaa ctatatcctg gcagaagtct aaatctcgat
      661 acattcgtaa atgaacttaa caaaactgat gaagctatga aaaaagtctt aggaaagaat
      721 ttttcctcaa atgtggttag atgcccaggt ggttatatgt catggaaaaa tatggaaccc
      781 ctaggaaact acttaaaaga gaaaaacatg gcatcaattg attggaatgc tttaaatgct
      841 gatgcagaag gtaaaaagaa aaatgcacaa gaattatttg aacatgcaaa gaaatcttct
      901 gaaggcaaag aaatggttgt tcttttaatg catgatactt atggaaaaca agaaacagta
      961 aatgcattgg accaaattat aacgtatttt aaagacaatg gttatcaatt taaaatttta
     1021 atatagtaaa aaagcaagtt tattgagtat acattttata ctcaataaac ttgcttttac
     1081 tttactctat tttactagct ctatttttac aaattttttc tttcctattt gaacaactaa
     1141 ttcactttct tttacaatag tgcttaagtc ctctactttt tcaccattta cctttacgcc
     1201 accttgagaa gctaatcttc taacttcact cttactctta acaatttcat taacaactag
     1261 tacttcgatt aagtcaaatc catcttcctt aacttgaatt gtttgtatat cttcaggtat
     1321 ttgacctttt tggaatacag atttaaatct ttcttcagct tctctagcag attcttctcc
     1381 atgatataat gttactattt ctcttgctaa gttcatcttt atatctcttg gatttacaga
     1441 accatctttt aattgagatt ctattttgtt aacttcatca ggatgaacat ctgttactaa
     1501 attgtagtat ttaattataa gttcatcagg aatttccata gatttttggt acattattcc
     1561 cgcttcttca tctattccaa tatagttacc taaactctta ctcatttttt cttttccatc
     1621 taatccttct attaaaggca tcattattac tatttgagac tccataccaa attctctttg
     1681 taacgatctt cccattaata agttgaatct ttggtcagtt ccaccaagtt ctatatccgc
     1741 ttctaatgct atagaatcat atgcttgcat taatggatag aaaaactcat gtactgatat
     1801 tggcatttgt ccttcatatc tctttttaaa atcttctctt tctaacattc tcgcaacagt
     1861 aatagttgct gctaatttta ttacatcttc aaaatttaac ttagctaacc actcactatt
     1921 aaatcttaca atagtttttt ctttatctaa aactttgaaa atttgttctt cataagtttt
     1981 tgcatttgca agaacttgct ctgttgttaa agcttttcta gctttagact ttccagttgg
     2041 atctccaatt tttcctgtaa agtctccaat tattattact atttgatgac ctaaatcttg
     2101 taattgtttc atctttctaa gaactactgt atgccctaaa tgtatatctg gagcacttgg
     2161 gtctagaccc aattttacta tcataggctt tccttctttt tctgatttaa taagtttttc
     2221 ccttaactct ttctcatcta ttaaatcatc tacacctttc ataattattc gcatttgctc
     2281 gtctatcgat ttcatttttc attcctccta aaatatttat ataatctata atttttattt
     2341 ttataataaa aaaagctttc atcctattaa aaggacgaaa gcattgctta cgtgttacca
     2401 ccttagttca tcaatatctc acgatattaa tctcttagag tataatggca tatactcgtg
     2461 tactataacg tgtactactc cgatatctct taatggttgt tatctttcag agatagtgct
     2521 ctgagatgta ttcaaactaa atcaatttat tccatttcac caaactggaa ctctctgtta
     2581 aataaaatct agctctactt cttctcttca tagcatttat atattcagat tatttcatca
     2641 attacaagaa atttttctta acttactcat aatatattat tatttttcta ttttgtcaac
     2701 ctttttatta ttatatacat gtttttttac attatactac ttttttatat tttttaccaa
     2761 tacctaaagt ttatttttca atcaaaagat ttatctgttt attgactata ttgttctgac
     2821 tcaatcttat atttattttc ttatttaaaa atgcattt
//
LOCUS       DAFNNB010000241         2847 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0247, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000241 DAFNNB010000000
VERSION     DAFNNB010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0247"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            22..1569
                     /locus_tag="L3M60_003432"
     CDS             22..1569
                     /locus_tag="L3M60_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBN6228052.1"
                     /translation="MNKNSMTKIYSLNKIIFIIIGILGIVLLTRHIGVGVKVLILILL
                     ILFSINVFISHLKIQVYEDKINLGLINNPDKFINITKDENFLKHVQNYIISNEKENCV
                     MACFDLCRLKLINDIYGYELGDEVLNNILSNLKYYFGKEAIYVKLKSAVFVLIIKLED
                     REQKIPYLVNLIKNKIVILNQIDNFKMDINIEASIGIYKFNNNEIDIKKAIDNADMAR
                     IKSKRLKHIEYVEFDNAMEEEIQSIMKIERDLFLAIKNKEFVIHYQPKVDSSTGNIIG
                     SEALIRWLHPSLGMIAPNKFIPIAEKNGLINNIGRWLIQEVFGTINKWILEGINVLPV
                     SINLSRVELYKNDLVDFFKLMFNTYNIPKELIELEITETTALRDVKFISERLYEIKSL
                     GMNISLDDFGVGNSNFINLKGIPLDVIKIDRSLILDIVTNTKTKFMVKAIVNLSHDLN
                     VTVICEGVEDMNQVKVLSELGCNFIQGYVFFKPLDEINYKRLLTDGSYINLEDTLLDK
                     CMAVEEE"
     gene            1572..1970
                     /locus_tag="L3M60_003433"
     CDS             1572..1970
                     /locus_tag="L3M60_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HBN6228053.1"
                     /translation="MISEDVDYSIRIIRAIFNNGEDNPIDAKMILKNEEIPTLTLYRL
                     LRKLCANNILTFNKEDGKKYMLNVSLDTISLRTIVEAIDGKIYINKCTRYVSNCSNCL
                     KGCKVNRELLEIEKEIYKLLSRKSIRQILE"
     gene            2010..>2847
                     /locus_tag="L3M60_003434"
     CDS             2010..>2847
                     /locus_tag="L3M60_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBN6228054.1"
                     /translation="MDDISQDNFLLSKEYENSLDVDTKKASGIYYTPKIIVDYIVKKT
                     LKNHDIIKNPYPRILDISCGCGNFLLEVYDILYDLFEENIYELKKKYDENYWTVDNIH
                     SHILNYCIYGADIDEKAISILKDSLINKKVVNDLDESDIKINLFCCDSLKKKWRYKFD
                     YIVGNPPYIGHKKLEKKYKKFLLEKYSEVYKDKADLYFCFYKKIIDILKQGGIGSVIT
                     PRYFLESLSGKDLREYIKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKKTYID
                     VFK"
BASE COUNT         1174 a          245 c          470 g          958 t
ORIGIN      
        1 ttaaaaaata ggggaattta tatgaataaa aatagtatga caaaaattta cagtttaaat
       61 aaaataattt ttattataat tggaatttta ggaatagttt tacttacaag acatattgga
      121 gtaggtgtta aggttttaat tttaatttta ttaatcttat ttagtataaa tgtatttatt
      181 tcacatctta aaattcaggt gtatgaggat aaaattaatt taggactaat aaataatcca
      241 gacaagttta taaatattac taaagatgag aacttcctaa aacatgttca aaactatatc
      301 attagtaatg aaaaagaaaa ctgtgtaatg gcttgttttg atttatgtag actcaaactt
      361 ataaatgata tatatggtta tgagctaggg gatgaagtat taaataatat tttaagtaac
      421 ttaaaatatt attttggaaa agaagctatt tatgtaaagt taaaaagtgc tgtttttgta
      481 ttaataataa aacttgaaga tagggaacag aagattcctt atttagtaaa tcttattaag
      541 aataaaattg tgatactaaa tcagattgat aattttaaaa tggatataaa tattgaggct
      601 tctattggaa tctataaatt taataataat gaaattgata taaaaaaagc tatagataat
      661 gctgatatgg caaggattaa atcaaaacga ctaaagcaca tagagtatgt agagtttgat
      721 aatgctatgg aagaagaaat acaatcaatt atgaaaatag agcgagattt attcttagct
      781 attaaaaata aagagtttgt aattcactat caacctaaag ttgattcaag tacaggtaac
      841 attataggtt cagaagcgtt aataagatgg ttgcatccat cattaggtat gattgcacca
      901 aataagttta tacctatagc tgaaaaaaat ggtctgataa acaatattgg tagatggcta
      961 attcaggaag tttttggtac cattaacaag tggattttag aaggtataaa tgtcttacca
     1021 gtttcaataa atctttccag agtagaatta tataaaaatg atttagttga tttttttaaa
     1081 ctaatgttta atacatataa tattcccaaa gaacttatag aattagaaat tacagaaaca
     1141 acagcactaa gagatgtaaa atttataagt gaaaggcttt atgagattaa atctttaggg
     1201 atgaatatat cattagatga ttttggagtg ggcaattcaa attttatcaa tcttaagggg
     1261 ataccactag atgttataaa aattgatagg tcacttatac tggatatagt tacaaataca
     1321 aaaacaaagt ttatggtgaa ggcaattgtt aacctttcac atgatttaaa tgtaaccgtt
     1381 atatgtgaag gggtagagga tatgaatcag gttaaagttt taagtgaact aggatgtaat
     1441 ttcatacaag gttatgtatt ttttaaacct ttagatgaga ttaattataa gagacttctt
     1501 actgacggaa gttatataaa tctggaagat actcttttag ataaatgcat ggcagttgag
     1561 gaggagtaag gatgatttca gaagatgtag attattcaat aagaatcata agagctatat
     1621 ttaataatgg agaagataat ccgatagatg ctaaaatgat attaaaaaat gaagaaatac
     1681 caacactaac actatacaga ttgcttagaa aactttgtgc gaataatata cttactttta
     1741 ataaagaaga tggtaagaaa tacatgttaa atgtatcatt ggatacaata agcttaagaa
     1801 ctattgtaga agcaattgat gggaaaatat atataaacaa atgtacgaga tatgtatcta
     1861 attgcagtaa ttgtttaaaa ggttgtaaag taaatcgcga attgttagaa atagaaaaag
     1921 aaatttataa acttttatcg agaaaatcta taagacaaat actagaataa aaaattttca
     1981 gctatgataa taattggagg ttttcggtta tggatgatat atctcaagat aattttctac
     2041 tgtctaaaga gtatgagaat agcttagatg tagatactaa aaaagcaagt ggaatatatt
     2101 atactcctaa aatcattgta gattatattg taaaaaaaac tttaaaaaat cacgatataa
     2161 ttaaaaatcc atatccacga atacttgata tttcctgtgg gtgtggaaat tttcttttag
     2221 aagtatatga tattttatat gacttatttg aggaaaatat atatgaattg aaaaagaagt
     2281 atgatgaaaa ttattggact gttgataaca tacacagtca tatattaaac tattgtatat
     2341 atggtgctga tattgatgaa aaagccataa gtatattaaa agatagctta ataaataaaa
     2401 aggtagtaaa tgatttagat gaatctgata taaagattaa tctattttgt tgtgatagtc
     2461 ttaaaaagaa gtggcgatat aagtttgatt acatagttgg taatccacca tatattggtc
     2521 ataaaaagct tgaaaagaaa tataaaaaat ttctcctaga aaaatatagt gaagtttaca
     2581 aggataaagc tgacttatat ttttgttttt ataaaaagat tatagatatt ttaaagcaag
     2641 gaggaattgg tagtgttata actccaagat atttcttaga aagtctatca ggtaaagatt
     2701 taagagaata tattaaatca aatgtcaatg ttcaagaaat agtagatttt ttaggtgcaa
     2761 atatatttaa aaatataggg gtatctagtt gtattctaac ttttgataag aaaaaaacaa
     2821 aaaagacata tatagatgta tttaaaa
//
LOCUS       DAFNNB010000242         2816 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0248, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000242 DAFNNB010000000
VERSION     DAFNNB010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2816
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0248"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(295..1002)
                     /locus_tag="L3M60_003435"
     CDS             complement(295..1002)
                     /locus_tag="L3M60_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HBN6228055.1"
                     /translation="MIFTKSNKYDKEFLMKNMMGPNCIKILEELTSKIKLEKGMRILD
                     LGCGKGISSIFLANEFDATVFATDLWIEPTENYKRFKEFKLDDKIFPIQAEAHELPYA
                     EGFFDAVISVDSYHYFGNKEGFLDNHISPLVKEGGILAMAMPGLKEDFVDCIPDELIP
                     FWQDNMNFHSIKWWNELWSDSESVTVEKCEALNCHDEAWQDWLNCDNSYARNDKKMME
                     VENGKYFNTISLVARVK"
     gene            complement(1916..2785)
                     /locus_tag="L3M60_003436"
     CDS             complement(1916..2785)
                     /locus_tag="L3M60_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBN6228056.1"
                     /translation="MVIIKAIELYKKLDLEFNIKNINDDWSFMNFENDFITTEFKEKY
                     IGLVLDNAQNINKVYTTTFPDKEIIKQVIDKDEKDILIFSHHAMGYIASDEGFPFHDI
                     PLSYMEEMKSRRISFYVLHSPLDNYSYYSTSVSFAKALGLEIVKPFCKYDDKIEVGVI
                     CKTSLKSIEEIKELIKKSVGHDVKLYDYGDSVLKDGLVAIAAGGGSYPFVAREVAELG
                     INLYITGFTKPLKHFEPVLEFHQIAKDNLINVIGATHYSTEKFACMSMVNYFKNLGLE
                     SEFLQGKYYLEDL"
BASE COUNT          956 a          471 c          259 g         1130 t
ORIGIN      
        1 ctttaatctt catcaatatc ttttttgcct tttattgtta taactgcttt tttattaatt
       61 ttttctcata aaataaacat ttagtattaa aatatatata gcgcaatagt atttgacatc
      121 taaccttaga aaaaataaaa atgcccaatt gcactaaaaa attcgattgg gcataaataa
      181 tataaaaaat cattttttat ataaactctt cttttaactt acgtttttat ttaacttctg
      241 ctttcattca acttatattt ctattcgttt aatactctca cttatcttat acttttattt
      301 aactcttgct accaaggaaa ttgtattaaa atactttcca ttctctactt ccatcatttt
      361 tttgtcattt ctagcgtacg aattatcgca gtttagccaa tcctgccaag cttcatcatg
      421 acaatttaaa gcttcacatt tctctaccgt cacagactca gagtcagacc aaagctcatt
      481 ccaccattta attgaatgga agttcatgtt gtcttgccaa aatggaatga gttcatctgg
      541 tatacaatcc acaaagtctt cttttaatcc aggcattgcc atagcaagta ttcccccctc
      601 ttttacaaga ggtgatatat gattatccaa aaaaccttct ttgtttccaa aatagtgata
      661 agaatctaca cttatcactg catcaaagaa accttctgca taaggtaatt catgtgcctc
      721 tgcctgtatt ggaaatattt tgtcatccaa tttaaactct ttaaatcttt tatagttttc
      781 agttggttct atccataaat ctgttgcaaa tacagtagca tcaaattcat ttgctagaaa
      841 tatcgaggat atacctttcc cacatcctaa atctaaaata cgcatcccct tttctagttt
      901 tatcttactt gttaattctt ccaatatctt tatacaatta ggtcccatca tatttttcat
      961 aagaaattcc ttatcgtatt tattactttt tgtaaatatc attctatctt tcctttccct
     1021 atcaattatt atttattaaa ttttatacgt ttgatttttt attttttata tgcttcttat
     1081 aatgagcaat ctctttttta ttcattcttt aacaggtaat tttaatgagt aattttatcc
     1141 actctacaat aatcacttta atgagtaatt ttatccactc tattatagtt attttaatgg
     1201 gtaattttat ccactctaca ataattactt taatgagtaa ttttatccac tctattatag
     1261 ttattttaat gggtaatttt atctactcta caataatcac cttaatgagt aattttatcc
     1321 actctattat agttatttta atgggtaatt ttatctactc tacaataatc accttaatga
     1381 gtaatcttat ccactctatt atagttattt taatgggtaa ttttatctac tctataatag
     1441 tcactttaat aagtaatcat aatttttata tcattttaga tacttactct aataagaagt
     1501 cgttttctga tacttgataa gttttaatta cttccacttt aacctatata ctaattaact
     1561 tatttaattt agtgtatatt tgacacttta ccttttatta taccatttat tctcataaat
     1621 ataataattc tattttttta aaataaatat ttataataat ctaaaataaa tatttataaa
     1681 ttctaatcaa tcataaataa taatctaaaa tatatcaaaa aataaactta aaaacctcga
     1741 aaaagaactt aaaagacatc aaaactaaag cttctaatca gcaattaaaa gtctattttc
     1801 ttagttaaaa aaataaagat agattttttc aaaacattgg ataaatgggt aaaaaaaata
     1861 aacagtcagt aaaacaaatt tttaccaact gtttctcttt attatttaat atatcctata
     1921 aatcctccaa gtaatacttt ccttgtaaaa attctgactc taaacctaga tttttaaaat
     1981 aattgaccat gctcatacat gcaaattttt ctgtagaata gtgagttgca cctataacat
     2041 taattaaatt atcttttgct atttgatgaa actctaacac tggttcaaaa tgttttaaag
     2101 gtttagtaaa tccagtaata tataggttta taccaagttc agcaacttct cttgctacaa
     2161 atggataact tcctccccct gctgcaatag caactaaacc atcttttaat acagaatctc
     2221 cataatcata caatttaaca tcatgaccaa cagatttttt aattaattcc ttaatctctt
     2281 ctattgattt tagtgatgtc ttacatataa ctcctacctc aattttatcg tcatatttgc
     2341 aaaatggttt gactatttct aaacctaggg cttttgcaaa actaacagaa gttgaatagt
     2401 aactatagtt gtctagtgga gaatgtaata cataaaaaga aattctacga cttttcatct
     2461 cttccatata agatagtggt atgtcgtgaa atggaaaacc ttcatctgat gctatgtatc
     2521 ccattgcatg atgtgaaaaa attaaaatat ctttttcatc tttatctata acctgtttaa
     2581 ttatctcttt atcaggaaaa gtcgttgtgt atactttatt aatattttga gcattatcta
     2641 atactaatcc tatgtacttt tctttaaatt ctgtagtgat aaaatcattt tcgaaattca
     2701 taaaactcca atcatcatta atatttttaa tattaaattc taagtctaat tttttataaa
     2761 gttctattgc cttgataata accactcctt tataacaaca attgattaat acatta
//
LOCUS       DAFNNB010000243         2807 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0249, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000243 DAFNNB010000000
VERSION     DAFNNB010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0249"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..258)
                     /locus_tag="L3M60_003437"
     CDS             complement(<1..258)
                     /locus_tag="L3M60_003437"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228057.1"
                     /translation="MKKITKKEIKKYVREAVNNNFNWEINKCGFYIKNNEIEFFISYK
                     GQGIDENVYNDTYEEIIYIEDIIEEYRRKEYNLEDVDSIVYD"
     gene            407..616
                     /locus_tag="L3M60_003438"
     CDS             407..616
                     /locus_tag="L3M60_003438"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228058.1"
                     /translation="MKDVFEELISIKDACELYKKGESTLRLNIRNGKFVEGVDCKKFG
                     TTWVFKKTALEREYNKKDKKIESGN"
     gene            complement(643..1449)
                     /locus_tag="L3M60_003439"
     CDS             complement(643..1449)
                     /locus_tag="L3M60_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6228059.1"
                     /translation="MKIVVTAGHTLTGKGTGATGYINEGTENRILMELIVKWLKKGGA
                     TVYSGKVDKSNNYLAEQCQIANKQNVDVAVQIHFNADHITLNKMGTETIYKTNNGKVY
                     AERVNEKLATVFKNRGAKSDVRGLYWLSHTKAPAILIEVCFVDSKADTDYYIRHKDIV
                     AKLIAEGILNKNIDNKENSEDKKMYKHTIVYDGEVDKIPATVVGWGYNDGKILICDIK
                     DYVPGKTENLYVVGGRACEKIGSMTKEKFTMIKGNDRFDTLYKALDFIKK"
     gene            complement(1452..1715)
                     /locus_tag="L3M60_003440"
     CDS             complement(1452..1715)
                     /locus_tag="L3M60_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228060.1"
                     /translation="MEQLLTELSSLGAIGILCALLFKNTMQEKKEDRDMYKKTVENFI
                     ELSTQQQEINKNILVEMGAMKTDVEEIKEDVIDIKDMLQKEGV"
     gene            complement(1857..2129)
                     /locus_tag="L3M60_003441"
     CDS             complement(1857..2129)
                     /locus_tag="L3M60_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228061.1"
                     /translation="MFDEEGEIIFYEGQSKGNVPERKNIKKIEYIDLEYDYVDYDKYK
                     IIGIDIRTKQPILEEIPVYMSEKEKRIQELENQILIAENEKVGGLL"
     gene            complement(2173..2784)
                     /locus_tag="L3M60_003442"
     CDS             complement(2173..2784)
                     /locus_tag="L3M60_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228062.1"
                     /translation="MIEKLNENASLSDLITAFENSTKELKASKDNFTNLLGNPFLEST
                     KFSEFEGKMQNLMSTFKDNLKSKGINSGNTESLLSLINKVANIYIPTPIYTASGECTI
                     DNKGTCKRYTISTAGDVYYVRINLNFYPKFIVMYNSGGASKYFTTSYFCSYVPFIAST
                     VESYTTYLLDKSDSRIQVQKGTCILPIVYSKYGSSSFYFYAVG"
BASE COUNT          849 a          506 c          278 g         1174 t
ORIGIN      
        1 gtcatataca atagaatcta catcttctaa attgtattct tttcttctgt attcttctat
       61 aatatcctca atgtaaatta tttcttcata agtatcatta tatacatttt catctattcc
      121 ttgacccttg taagaaataa aaaattctat ttcattattt ttaatgtaaa acccacattt
      181 atttatttcc cagttaaaat tgttattaac cgcttctcta acgtactttt taatttcttt
      241 cttagttatt tttttcattt ttaaatcccc ctttgtttat ctttctttaa tcatattata
      301 aacgcttacg tttattaagt caatagtttt ttaaacataa acgtttattt ttttcttgtc
      361 tttttaaact tttgcgatta taatataaat aaaaggagtt gttaaaatga aagatgtatt
      421 tgaggaacta atctctataa aagatgcttg tgaactttat aaaaaaggtg aaagtacgtt
      481 aagacttaat ataagaaatg gaaaatttgt ggaaggtgta gactgtaaaa aattcggaac
      541 gacatgggtt tttaaaaaaa cagcacttga aagagaatat aacaaaaagg ataaaaaaat
      601 agagagtggt aactgatgaa atagttacca ctctctacaa acttatttct taataaaatc
      661 taatgcttta taaagtgtat caaatctatc attacccttt atcatagtaa atttttcttt
      721 agtcatagaa ccaatcttct cacatgctct acctcctaca acatataaat tttctgtctt
      781 acctggtaca taatctttta tatcacatat cagtattttt ccatcattat agccccaacc
      841 aactacagtc gcaggaattt tgtcaacttc tccatcataa acgattgtat gtttatacat
      901 tttcttatcc tcactatttt ctttattatc tatattttta tttaaaatac cttctgctat
      961 taacttagcg actatgtctt tatgtctaat ataatagtct gtatctgctt tactatctac
     1021 gaaacacact tctattaata tcgctggagc ttttgtatga ctaagccagt aaagccctct
     1081 gacatctgat tttgcacctc tatttttaaa tactgttgct agtttctcgt tgactctttc
     1141 agcatatacc ttaccattat tagttttata aattgtttct gtacccattt tatttaacgt
     1201 tatatggtca gcattaaaat gtatttgtac agctacatct acattttgtt tattggctat
     1261 ttgacattgc tctgctaaat agttattaga tttatctact tttccactat acacagtagc
     1321 cccacccttt ttaagccatt taacaatcaa ttccatcagt atcctatttt ctgttccttc
     1381 atttatatat cctgttgctc ctgttccttt tcctgttagc gtatgccctg ctgttactac
     1441 aatcttcata cttaaacccc ttccttttgc aacatatctt ttatatctat tacatcttct
     1501 ttgatttctt ctacgtctgt tttcattgct cccatttcaa caagtatatt cttatttatc
     1561 tcttgttgct gtgtagatag ttctatgaaa ttttctactg tttttttata catatctcta
     1621 tcttcttttt tctcctgcat ggtattttta aatagcaaag cgcataatat gcctattgct
     1681 cctaaactac ttaattctgt taataattgt tccataattt tcctccattt ttttatcaaa
     1741 ataaaactta taaattatct aattctataa aaaaagaacc ttatacggtt ctgcttcttt
     1801 tactacttca ctatttttta ttagctaata ttcttactac aacattattg tgttcgttat
     1861 aatagtcctc ctactttttc attttctgca atcaatattt gattttctaa ttcttgtatt
     1921 ctcttttctt tttcactcat atatactggt atttcttcta agattggttg cttagttctt
     1981 atatctattc ctattatttt gtacttatca taatcaacat aatcgtattc caaatctata
     2041 tattctattt ttttaatatt ttttctttcg ggaacattcc cttttgattg tccttcatag
     2101 aatattattt cgccttcttc atcaaaaata tccttcttcc tacttctata taatcattca
     2161 taaaaccact ccttaaccta ccgcataaaa ataaaaacta gaactaccat acttgctgta
     2221 aactataggt aaaatacatg ttcctttttg aacttgtatc ctactatcgc ttttatcaag
     2281 caagtaagtc gtataacttt ctactgttga agcaatgaaa ggaacataac tacaaaaata
     2341 gctggttgta aaatatttac tagctccacc ggagttatac ataacaataa atttcggata
     2401 aaaatttaag ttaattctaa cgtagtaaac gtctccagct gtactaattg tatatctttt
     2461 gcatgtacct ttattatcaa tagtacactc gccacttgct gtatatatcg gcgtaggtat
     2521 atatatattt gcaactttat taattaaaga caataaagac tctgtattac cactatttat
     2581 gcctttactt tttaaattat ctttaaaagt actcataaga ttttgcattt taccctcaaa
     2641 ctccgagaat ttagtacttt ctaaaaatgg attgcctagc aaatttgtaa aattatcttt
     2701 acttgctttt aattcttttg tactattttc aaaagcggtt attaagtcac ttaaactagc
     2761 attttcgttt aatttttcta tcataaaaat atcacctttc tattttt
//
LOCUS       DAFNNB010000244         2801 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0250, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000244 DAFNNB010000000
VERSION     DAFNNB010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0250"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            118..873
                     /locus_tag="L3M60_003443"
     CDS             118..873
                     /locus_tag="L3M60_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003399531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBN6228063.1"
                     /translation="MLQIKNLSKSFPNPYGEPNTIFENLSIDIEDGEFVSIIGSNGTG
                     KSTLLNIISGLIKESSGQITLDNTTITNLAEYKRTQIVSRVFQDPSLGTCPSMTVREN
                     LSLALNKGKLLNLKKCLRHKTNDLEHLLEGISLDLKKYLDIKVQYLSGGQRQSLSLIM
                     SSLASPKVLLLDEHTAALDPKTSNEVIELTDKIVREKNITTLMVTHNLKHALQYGDRL
                     IMLHKGEVVLDVKDKEKEDLTVEEILEKFEYAV"
     gene            969..2006
                     /locus_tag="L3M60_003444"
     CDS             969..2006
                     /locus_tag="L3M60_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBN6228064.1"
                     /translation="MIKSKKILSLIIAGVLGISMLTGCSKNDGSNASNENKKTDIKNQ
                     KNIGISQLVEHPSLDKAKKGFIKALEDKGYKDGDNIKIDFQNAQNDMPTTQSIASKFA
                     SDKKDLIYAISTPSAQAAYNATKDIPIIMTAVTDPVEAGLVKSLEKPGGNVSGTSDYL
                     SIDKTLELVKTLTPKAKKIGVIYNTSEVNSKIQVDSLHDYAKKNNYEVVEKGISSSSE
                     VNQAISSLVGKIDVLYVPTDNLIVSSMPIVSKVANENKIPIIASEEGSVSSGALACCG
                     IDYEKLGYKAGELAIEVLEGKSVGDIPVTTLDETEIIINEDTLKALDMQKLSADNIKY
                     ITSDENAKSAK"
BASE COUNT         1108 a          361 c          411 g          921 t
ORIGIN      
        1 taataactgc catagttata atagctttct tagctactgg taacttaaat atttcactaa
       61 aaaaaaacaa atgcaaaact agtaccaaaa ataaatcaga aaaaagaggt gttatagatg
      121 ttacaaatta aaaatctttc aaagagtttc cctaatccat acggtgaacc taatactata
      181 tttgaaaatt tatcaatcga tatagaagat ggtgaatttg taagtatcat aggtagtaat
      241 ggaactggta aatctacttt attaaatata atctctggac ttataaaaga atcttctggt
      301 caaattactt tagataatac cactataaca aatttagctg agtataaaag aactcaaata
      361 gtaagtagag tttttcaaga cccaagcctt ggaacttgtc cttctatgac ggttagagaa
      421 aatctatctt tagcacttaa taaagggaaa ttattaaact taaagaaatg tcttcgtcat
      481 aaaacgaatg atttggaaca cttacttgaa ggaatttctt tggacttaaa aaaatactta
      541 gatattaaag tacaatatct ttcaggaggt caaaggcaat cactctcact aattatgtct
      601 agtttagcaa gtcctaaggt cttattgcta gatgaacata cagcagcact tgacccaaaa
      661 acatcaaatg aagtaataga gttaacagat aaaatcgtaa gagaaaaaaa tataactaca
      721 cttatggtta ctcacaactt aaaacatgct cttcaatatg gtgatagatt gataatgctt
      781 cataaaggtg aagttgtctt ggatgtaaaa gataaagaaa aagaagattt aacagttgaa
      841 gaaatcttag aaaagtttga atatgcagta taagaaatta aaatcttagt tttaatcagg
      901 aatatttata atctattata tgaatatatg aacaaatata attattaaat tttgagggag
      961 gaaataaaat gataaaaagt aaaaaaatat taagtttaat tatagcaggg gtacttggta
     1021 tatcaatgct tactggatgt tccaaaaatg atggctctaa tgcctcaaat gaaaataaaa
     1081 aaacagatat caagaatcaa aaaaatatag gtattagtca attggtagaa catccatctt
     1141 tagataaagc taaaaaagga ttcatcaaag cactcgaaga taaaggctat aaagacggag
     1201 ataatataaa aatagatttc caaaatgcac aaaatgatat gcctactaca caaagtattg
     1261 ctagtaagtt tgcatccgac aaaaaagatt tgatatatgc tatatctact ccatctgcac
     1321 aggcagctta taatgctact aaagatatcc cgataataat gactgctgtt acagaccctg
     1381 tagaagctgg acttgttaaa tctcttgaaa aaccaggtgg gaatgtttct gggacatctg
     1441 actatctttc aattgataaa acattagaat tagttaaaac attgactcca aaagcaaaga
     1501 aaataggagt tatatacaat actagtgaag ttaattcaaa aatccaagtt gattctctac
     1561 atgattatgc taagaaaaat aattatgaag tagttgaaaa aggaatcagt tcttcaagtg
     1621 aagttaacca agctatttct agtttagttg gcaagataga tgttttatat gtacctactg
     1681 acaatttaat agtttcttct atgccaatag tttctaaagt tgctaatgaa aacaagattc
     1741 ctataatagc ctctgaagaa ggttctgtat cttctggtgc tttagcttgt tgtggaatag
     1801 attatgaaaa actaggttat aaggctggag aacttgctat tgaagtatta gaaggaaaat
     1861 ctgttggcga tatacctgtt actacattag atgaaactga aataataatt aacgaagata
     1921 cactaaaagc acttgacatg caaaagttat cagcagataa tataaagtat ataacgtcag
     1981 atgaaaatgc aaaatctgca aaataaaact tattaatgtc aaaaaatatg taatatgaag
     2041 taggtaatat gaaaacttta tataataagt actcttatcc accctaaccc tttgaaaatt
     2101 gtgcttattt tagcaatttg aatattgcat ataaagttat tcattttcca taaaagtcgt
     2161 ctatattgac gactgattat gaaagttata taactttcta atcactactt gagctgtcca
     2221 aaagaatttt aatattctct agacagcttt tttttatgag atttgattga tatatatgtc
     2281 tagttattcc atacttagta aatattttga taaaatttcc acatccacca caacttagga
     2341 taacgttttt tccaaataat atattaattt aaaagtattc taagtatata ttatttagta
     2401 ttttaattaa atttagcagt tgacttttat tattgtataa tttataattt ataacaagtc
     2461 gaaaatacat ataattttct gactaattaa aatattaaaa gggagagata attatgaata
     2521 ctttattaaa caataaaaaa tattattatt attatagcca ctatttttag ggtttgtacc
     2581 tacgttttaa catagataca gaccccttaa gactactaaa attttgggat tgtatctatg
     2641 ttggctatat tgtttataaa gttttcatgt aactatataa gagccacata gattaaacta
     2701 tgtggcttat tcgtgttttt accacatagt tttgtacact atgtggtttt tttatacaca
     2761 aaattagcaa ataattaaaa ttataaaaat attttcaaag g
//
LOCUS       DAFNNB010000245         2790 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0251, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000245 DAFNNB010000000
VERSION     DAFNNB010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0251"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            477..2396
                     /gene="cooS"
                     /locus_tag="L3M60_003445"
     CDS             477..2396
                     /gene="cooS"
                     /locus_tag="L3M60_003445"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HBN6228065.1"
                     /translation="MDEKMLTIDLNSQKMIAKAREEGVETMYDRKEGFKAQCGFGLQG
                     VCCRICGMGPCRISPKTPRGLCGADEHTIVGRNFARMVAGGTAAHSDHARDIAHTLAL
                     ADPNGNYKIRDEAKLITLAKEWDVETEGRDIYDVAHEVAEIALMEFGKPFGTLRFIKN
                     APEPRQKIWKEYAIEPRAIDREIATIMHSTHIGCTGDIDSLIHMSLRTSMADGWGGSM
                     IGTRLSDILFGTPVPRRTEANLAVLEENKVNIILHGHEPALSEMIVLASEEPDLVALA
                     KEVGADGINLAGMCCTGNEITMRHGVKIAGDFHQQELAIITGAVEAVIVDVQCIFPAL
                     ARVADCYHTKFVTTSPKAKITGSTYIEFREEQALDDAKAIVKEAILNFKNRDKSKVLI
                     PELKSGATVGYSVEAVVNQLDRVVNSHIDPAGTVKPLTDCLKAGVLRGAAGVVGCNNA
                     KGVSNEAHVTIMKELIKNDIIVVTTGCGASAAAKFGLMEADAAEKYAGKGLATVCKLV
                     GIPPVLHMGSCVDISRILDLVGAAANYLDMDMCDLPVVGIAPEWMSEKAVAIGCYVVA
                     SGIDTYLGIMPPIAGSSRAVDILTSELKDKVGATFTVNTNPKELAATIIEDIEKKRVH
                     FEALVEEKMAEKAEA"
     gene            2705..>2790
                     /locus_tag="L3M60_003446"
     CDS             2705..>2790
                     /locus_tag="L3M60_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase"
                     /protein_id="HBN6228066.1"
                     /translation="MKIAITGKGGVGKTTFSSMLSRMFAEDG"
BASE COUNT         1122 a          336 c          560 g          772 t
ORIGIN      
        1 gaagctataa ttaatattaa agtaggaaaa ataaaaaaat tcaaaaaaat tagtccaaaa
       61 aatgctattc taagaagttt ttactaatta agtttaggga gcaaaaatct gtaataagtc
      121 caaatagtag tgaaagataa taaatacaca aacaatctgt aataatataa aaatgactag
      181 aaaaatttca aatttgattt aaaaaaatca aaacataatt taattaaact gaagtatcaa
      241 aaaagtaggt ttagatatta acaaatagcc aaaaataagc gataaaatca acttaaatta
      301 agtacaaaaa ataaaatttt tatgtattat ctaattttgt taaaaaaata tttaaatcta
      361 aagcaaagca ctgaaaatta gtatataatc aacttagaaa gcatttattt tattatttta
      421 tgacatgaag tcagtcacct gggggatttt aaaaatcaaa aaaaggggat aggaatatgg
      481 atgaaaaaat gttaacaatt gatttaaaca gccagaagat gatagctaaa gctagagaag
      541 aaggcgtaga aacaatgtat gatagaaaag aaggctttaa ggcacaatgt ggttttggtc
      601 ttcaaggtgt ttgctgtaga atatgtggaa tggggccatg tagaataagc cctaagactc
      661 caagggggtt atgtggagca gatgagcata ctatagtagg tagaaacttt gcaagaatgg
      721 ttgctggtgg gacagctgcc cattcagacc atgctagaga tatagcacat acattagcat
      781 tagctgatcc aaatggaaac tataaaataa gagacgaagc taagttaatt actttagcta
      841 aagaatggga tgtagagaca gaaggtagag atatctatga tgtagcacat gaagtggcag
      901 aaattgcatt aatggaattt ggaaaacctt ttgggacatt aagatttata aaaaatgcac
      961 cagaaccaag acaaaaaata tggaaagaat atgctataga gccaagagct atagatagag
     1021 aaattgcaac aataatgcac tctactcata taggatgtac tggtgatata gatagtttaa
     1081 tacacatgag tcttagaact tctatggctg atggttgggg tggttctatg ataggtactc
     1141 gtctaagcga tatcttattt ggaactccag tgccaagaag aacagaggct aacttagcag
     1201 tattagaaga aaataaagtt aatataatat tacatggaca tgagccagca ttatcagaaa
     1261 tgatagtttt agcatcagaa gaaccagacc tagtagcttt agctaaagaa gttggagctg
     1321 atggaataaa cttagcaggt atgtgttgta caggtaatga gattacaatg agacatggtg
     1381 taaaaatagc aggagacttc catcaacaag aacttgcaat aataactgga gcagttgaag
     1441 ctgtaatagt tgatgttcaa tgtatattcc ctgcattagc tagagttgca gattgttacc
     1501 atactaaatt tgtaacaact tctccaaaag ctaagataac aggttcaact tatattgaat
     1561 ttagagaaga acaagcactt gatgatgcta aagcaatagt taaagaagct attttaaact
     1621 ttaaaaatag agataaatca aaagtactta tacctgaatt aaaatcagga gcaacagttg
     1681 ggtatagtgt agaagcagta gtaaatcaat tagacagagt tgtaaactct catatagacc
     1741 cagcaggaac agttaagcca ttaacagact gtctaaaagc tggagtatta agaggagcag
     1801 ctggtgtagt tggatgtaac aatgctaaag gagtttcaaa tgaagctcac gtaacaataa
     1861 tgaaagaatt aatcaaaaac gacataatag tagttactac aggatgtgga gcttcagcag
     1921 cagctaagtt tggtttaatg gaagctgatg cggctgaaaa atatgcaggt aaaggtcttg
     1981 caactgtctg taaattagta ggaatcccac cagtactaca tatgggttct tgtgttgata
     2041 taagccgtat actagattta gttggagcag cagctaatta cttagatatg gatatgtgtg
     2101 accttccagt tgtaggtata gctccagagt ggatgtcaga aaaagcagtt gctataggat
     2161 gctatgttgt agcttctggt atagacactt atctaggaat aatgcctcca atagctggtt
     2221 cttcaagagc agtagatata ttaactagcg aattaaaaga taaagttgga gcgacattta
     2281 ctgtaaatac aaatccaaaa gagttagcag ctacaataat agaagatata gagaagaaac
     2341 gtgttcactt tgaagcatta gttgaagaaa aaatggcgga aaaagctgaa gcttaagaaa
     2401 tagcgaagtt atagaataaa cgtttttata agaagtttat ggctgaataa agttgaataa
     2461 cgtaaaatat taaagtaaag aacttaaaat ataatgaata tactgaataa ataaaatata
     2521 aacaatatta aagaataata aaactataca tgctgctttg aaaagctagt agggtaaatc
     2581 taattggttc ctgtaaggac tagctgtagt ttatttttag tgtataaaaa tagagagtat
     2641 aaaaaatatt taactataaa attaactact aaaaagcaaa tagtaataaa aggtggtatg
     2701 gattatgaag atagcaataa caggtaaagg tggagtaggt aaaacgactt tttcttcaat
     2761 gctctctagg atgtttgctg aagacggata
//
LOCUS       DAFNNB010000246         2769 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0252, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000246 DAFNNB010000000
VERSION     DAFNNB010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0252"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(201..1163)
                     /locus_tag="L3M60_003447"
     CDS             complement(201..1163)
                     /locus_tag="L3M60_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HBN6228067.1"
                     /translation="MRVLNNVTEAIGNTHMLHLSKIVDEFGVQGNIYAKMEYLNPGFS
                     KKDRVALQMITEAENAGLLVPGQAVVELTSGNTGTGLAIACACKGYKFIACMSKGNSM
                     ERARMMKALGAEVVLVDQMPNSVHGQVSGEDLALVEIEAQRITKERNAFRADQFNLEA
                     CNHAHENYTAEEMWEQLDGNIDVFVDFLGSGSTFAGCSKALKGHKSSIKCYVVEPKTA
                     AIYSGKEITDQGHKIQGGGYSMDLPLVDKKLIDGYIQITDEEATDVARKLAKLEGVFA
                     GFSSGANVCAAIKLLKSTERGKNIVLTLNDSGLKYLSTDLYEEL"
     gene            1354..1905
                     /locus_tag="L3M60_003448"
     CDS             1354..1905
                     /locus_tag="L3M60_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228068.1"
                     /translation="MSINTIIAKNLNRLRNERNLSLGQLAELSGVSKVMLSQIEKGDS
                     NPTVNTIWKIASGLNVPYTAILEQPQNETFIVSKTDIDVQVSENKDYRLYCYYPNTPT
                     RNFELFQMELEEGHSYTSVGHSEKSQEYIMIIEGQLKLEVNDSIYQLRENDSICFSAE
                     SMHTYHNQGEKTLKAVIINYYPV"
     gene            complement(2440..2727)
                     /locus_tag="L3M60_003449"
     CDS             complement(2440..2727)
                     /locus_tag="L3M60_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228069.1"
                     /translation="MKKFLYALYSFIVIIANFRLKEKNYNFILLAIFFILLLIQNGVL
                     KTGYQNRATFYQDNYFNGEDKNDKIKFMTIKIFIFLSLPLCFNILFDYLTS"
BASE COUNT          971 a          439 c          299 g         1060 t
ORIGIN      
        1 attataatat gtaaatacaa aaacatatat tcatttttat tttatgacta agcataatta
       61 tctatataaa tttatgctta tagtcatgat actacatgtt tattaaaaaa aaaagtatct
      121 cattaatttt taaaaatcat tttgagatac tttttacaat ttaaatccat atatttaact
      181 taagaaacac tgtatcatta ttataattct tcatataaat cagtactcaa gtatttcaga
      241 ccactatcat taagagtcaa aactatattt ttacctcttt cagtactctt taataactta
      301 attgctgcgc atacatttgc tccagaagaa aaacctgcaa atacaccttc taatttagct
      361 aattttcttg ctacatcagt tgcctcctca tctgtgattt ggatataacc atcaataagt
      421 ttcttgtcta ctagtggcaa gtccatagaa tatcctccac cttggatttt atgaccttgg
      481 tcagttattt ctttacctga gtaaattgct gctgttttag gttcaactac atagcatttt
      541 atagaagatt tatgtccttt aagcgctttt gagcaaccag caaaagtact accacttcca
      601 agaaaatcta caaatacatc aatatttcca tccaattgtt cccacatttc ttctgctgta
      661 taattttcat gtgcatgatt acaagcttct agattgaatt ggtctgctct aaatgcattt
      721 ctttcttttg ttattctttg agcttcaatt tctacaagcg ctaaatcttc accagatact
      781 tgtccatgaa ctgagttagg catttggtct acaagaacta cttctgcacc taaagctttc
      841 atcattcttg ctctttccat agaatttcct tttgacatac atgctataaa tttataacct
      901 ttacatgcac aagctattgc taaccctgtt ccagtattac cacttgttaa ttccacaact
      961 gcttgacctg gaactaataa gcctgcgttt tctgcttcgg taatcatttg taatgctaca
     1021 cggtcctttt tgctaaatcc cggatttaag tattccatct ttgcataaat attaccttga
     1081 actccaaatt catctactat tttggataaa tgtaacatat gagtattgcc tatagcctct
     1141 gtaacattat taagtacacg cattacaatt cccccttaat ctctcaacta tcatccgttg
     1201 aaaaacaatt ctttatgcag tataatttat attacggatg tacagtgtat ttaacgttca
     1261 ttatattata catttgtaaa ttataaataa cgttaaataa attatatatt cgtaaattat
     1321 atttgtcaat aatttacatt ttaaggagct aatatgagta ttaatactat tattgctaaa
     1381 aacctaaata gattacgtaa tgaacgtaac ttaagtctag gtcaacttgc cgaattgtct
     1441 ggcgtaagta aagtaatgct ttcgcagatt gaaaagggag actcaaaccc cacagtaaac
     1501 acaatttgga aaattgcaag tggactaaat gttccatata ctgcaatact ggaacaaccc
     1561 caaaatgaaa cttttattgt atcaaaaact gatatagatg tacaagtttc agagaataaa
     1621 gattatcgtc tttattgtta ttatcctaat acaccaacta gaaacttcga attatttcaa
     1681 atggaattag aagaaggtca tagctataca tctgttggtc attctgaaaa atcacaggaa
     1741 tatattatga ttatagaagg tcaattaaaa ttagaagtaa atgactctat ttatcaactt
     1801 agagaaaatg actctatttg tttttcagcc gaatcaatgc atacatatca taatcaagga
     1861 gaaaaaacat taaaggcagt aataataaat tattatccag tttagccatg tcatttaatg
     1921 atagaataat tattttctat acaaaaagat aaatcctttg attaagattt atctttttca
     1981 ttttattttt ctttataaat tcattatata tctttacact tttatcaatt atgttataat
     2041 aagcttatat aaaagtttta tatttttgac aaatggagta caaaatactc ctttttcttt
     2101 tttactattg tgataattat ttagttagta ttaaaatatt atcacttaaa acaaatacaa
     2161 aacgtctctt atatgtacat ttttcattgt tgtaataatg atttgtgtat gtatttctca
     2221 caatcggagt gcttatgcac tctttttaaa tacctttatt accacaatct aaaagctatt
     2281 ttattatcca ttttaattta tttttaagta atcaaataaa ttattttata ttttttcttc
     2341 atttatatga aactttttta tcactatatg cactttatat ttaatataaa taacacataa
     2401 gcttgctttt atcataacac tccaatattt aaatataatt taagaagtta aatagtcgaa
     2461 tagtatatta aagcataatg gtaatgacaa aaatataaaa atctttatag tcataaactt
     2521 tatcttatca tttttatctt ctccattaaa ataattatct tgataaaatg tagctctatt
     2581 ttgataacct gtttttaaaa ctccattttg aattaatagc agtataaaaa agattgctaa
     2641 taaaataaag ttataatttt tttcttttaa tctaaaattc gctataataa caatgaaact
     2701 atataaagca tataaaaatt ttttcataaa aaacacctaa attctatatt tgtattttat
     2761 attgagcca
//
LOCUS       DAFNNB010000247         2708 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0253, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000247 DAFNNB010000000
VERSION     DAFNNB010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0253"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            191..496
                     /locus_tag="L3M60_003450"
     CDS             191..496
                     /locus_tag="L3M60_003450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228070.1"
                     /translation="MNINKYEKLCEQQELLYEQYKETKEKIKVYEVLEDRRKGIDTMI
                     RNIDSYFGIGELSMEFNIRGSCDVSCVHFSVGEEEKILLKNFFIELKEKNVRKMEEI"
     gene            511..678
                     /locus_tag="L3M60_003451"
     CDS             511..678
                     /locus_tag="L3M60_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228071.1"
                     /translation="MDKFVTCARCERTINVEENNYVKYEEETLNLSFALYFCLGCVDK
                     IIEEQVEGENE"
     gene            671..886
                     /locus_tag="L3M60_003452"
     CDS             671..886
                     /locus_tag="L3M60_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228072.1"
                     /translation="MNKISVTQALEKFDSLLDNWNDLPNHVYKKEYRGKFYDWIKSLE
                     RKDSLQNYKIVEVLNDERNGEEAPFWN"
     gene            918..1325
                     /gene="ssb"
                     /locus_tag="L3M60_003453"
     CDS             918..1325
                     /gene="ssb"
                     /locus_tag="L3M60_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBN6228073.1"
                     /translation="MNNVAMIGRLTKDPELKYIPSSGTAVSTFTIAIDRDYIKKDGNK
                     ETDFIPVEVMGKLAEVCANNLCKGRLVAVEGSIRVNSYEKEGEKRTYTKVHANKIKFL
                     DYKKEDNEKEYMFEPKGLDQQGFQAIDDPDIPF"
     gene            1342..1491
                     /locus_tag="L3M60_003454"
     CDS             1342..1491
                     /locus_tag="L3M60_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228074.1"
                     /translation="MSNINKKTAKICGWCGTTVYSYVDGKIAYCSKKCKKKREKNLEI
                     EGAKK"
     gene            1488..1769
                     /locus_tag="L3M60_003455"
     CDS             1488..1769
                     /locus_tag="L3M60_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HBN6228075.1"
                     /translation="MKYIASFSGGKDSAAMLLLILEKKLPLDEIVFIDTGLEFEEIYN
                     IIDDFEKRINFKITRIKAEKTFEEYFYTVNKQGKRKGQIWGFPYLLGAW"
     gene            1890..2180
                     /locus_tag="L3M60_003456"
     CDS             1890..2180
                     /locus_tag="L3M60_003456"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228076.1"
                     /translation="MKKTKIEEFITKVKESMQSVAKESIKDLENILKEIETGETEVKI
                     LFIDKEKKVEEIVEFTHDEYVKMIIEKMIFDSLKIIKEQHEKILKEFEEREV"
     gene            2183..2443
                     /locus_tag="L3M60_003457"
     CDS             2183..2443
                     /locus_tag="L3M60_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016056420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228077.1"
                     /translation="MNFKMIANIPKWTVKEQFEKVLEEVLELKEAIELKDNKKIMEEG
                     LDVIQAIFTLFKVLKIDKEIREGLKLHNKKLRKRKWKLEKIN"
     gene            2461..2664
                     /locus_tag="L3M60_003458"
     CDS             2461..2664
                     /locus_tag="L3M60_003458"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228078.1"
                     /translation="MSMCRKCLCGDYLDELVVGECILCGQEFFSIKTPVGKMCPSCSE
                     MFKGNCSQCAKPIGNEKSYLKIN"
BASE COUNT         1216 a          217 c          519 g          756 t
ORIGIN      
        1 aattagaaac ttataattta ttcatagatt gtattttaaa caaaaatata aggaaagcta
       61 ttgcgattta taacaggact aaaagtatta gaattaaaaa taaaaatatt aattctatat
      121 ctaataaaat tgaaaaaata aagcaaataa tttccttgta gaaataaaag atttaaaaag
      181 gagcgcaaaa atgaacataa ataaatatga aaaattatgc gagcagcagg aattactgta
      241 tgaacaatac aaagaaacaa aagaaaaaat taaggtatac gaggttttag aggatagaag
      301 aaaaggcatt gatacaatga taagaaatat tgattcttat tttggaattg gagaattaag
      361 tatggagttt aacatacgag gttcttgtga tgtatcttgc gttcattttt cagtaggaga
      421 agaagaaaaa atactattaa aaaatttttt tatagaacta aaagaaaaaa atgttcgaaa
      481 aatggaagaa atataaggag aggtttaata atggataagt tcgtaacatg cgctagatgc
      541 gaaagaacaa taaatgtaga agaaaataat tatgtgaagt atgaagaaga aactttaaat
      601 ttaagttttg cgttgtattt ctgtttaggc tgcgtagata aaataattga agaacaagtg
      661 gagggagaaa atgaataaga ttagtgttac acaagcatta gagaaatttg atagtttact
      721 agataattgg aacgaccttc cgaatcatgt ttataaaaaa gaatatagag gtaaatttta
      781 cgactggata aagtcacttg aaagaaaaga tagtttacaa aattataaga tagttgaagt
      841 tttaaatgat gaaagaaatg gagaagaagc gccattttgg aattgaagaa atataaaaaa
      901 gtgaaaggtg gtttaaaatg aataatgttg caatgattgg aagactaaca aaagaccctg
      961 aattgaaata tataccaagt tctggaacag ctgtatctac ttttacaata gcaatagata
     1021 gagattatat caaaaaagat ggcaacaagg aaactgattt tattcctgtt gaagtaatgg
     1081 ggaaattagc ggaagtatgt gctaataatt tatgtaaagg taggttagta gcggttgaag
     1141 ggtctataag agttaatagt tatgaaaaag aaggcgagaa gagaacttat acaaaagttc
     1201 atgctaataa gattaaattt ttagactata aaaaagagga taatgaaaaa gaatatatgt
     1261 ttgaaccgaa aggtttagac caacagggat ttcaagctat agatgacccg gatataccat
     1321 tttaaagcgg gagggataat aatgtctaat ataaataaaa aaacagctaa aatttgcggt
     1381 tggtgtggaa caacagtcta ctcttatgta gatggtaaaa ttgcatattg ttctaagaaa
     1441 tgtaagaaaa aaagagaaaa aaatttagaa atagaagggg cgaaaaaatg aagtatattg
     1501 caagttttag tggaggaaaa gattcagcag caatgctcct tctaatatta gaaaagaaat
     1561 tacctcttga tgaaattgta tttatagata caggactaga atttgaagaa atttataaca
     1621 taatagatga ttttgaaaaa agaataaact ttaaaataac aagaattaaa gcagaaaaaa
     1681 cctttgaaga atacttttat actgttaata aacaaggtaa gcgtaaaggg caaatatggg
     1741 gttttcctta tcttttaggg gcatggtaaa agtgaaatat ctgaatatat agaaaaaatt
     1801 ggtggcaaac aattaaattt tataaaaaga agagatagtg acttaacagt tgaaagttgc
     1861 aggagaataa aataaggaga aaaaataaaa tgaaaaaaac aaaaatcgaa gaatttatta
     1921 ctaaagttaa ggaaagtatg caaagtgtag caaaagaaag tataaaagac ttagaaaata
     1981 ttttgaaaga aattgaaact ggagaaacag aagttaaaat tttatttatt gacaaggaaa
     2041 aaaaagttga agagattgtt gaatttacac atgacgaata cgtgaaaatg attattgaaa
     2101 aaatgatatt tgatagtctt aaaataataa aagaacaaca cgaaaagatt ttaaaagagt
     2161 tcgaagaaag agaggtctaa aaatgaattt taaaatgatt gcaaatatac caaagtggac
     2221 agtaaaagaa caatttgaaa aagtgcttga agaagtttta gaacttaaag aagctattga
     2281 attaaaagat aacaaaaaaa taatggaaga aggtttggat gttattcaag caatttttac
     2341 actatttaaa gtattaaaga ttgataaaga aatacgcgaa ggtttgaagc tacataataa
     2401 aaaactaaga aaaagaaaat ggaaactcga aaaaataaat tagcataggg gggagttaaa
     2461 atgagtatgt gtagaaaatg tttgtgtgga gactatttag atgaactagt agtaggcgaa
     2521 tgtattttat gtggtcaaga atttttttct ataaaaactc cagttgggaa aatgtgtcct
     2581 agctgttcag aaatgttcaa ggggaattgt tctcaatgtg caaaaccaat tggaaatgaa
     2641 aaaagttatt taaaaataaa ttaaagtaaa taggcaatat aaaacgcaat tagattaatt
     2701 tttaaatt
//
LOCUS       DAFNNB010000248         2680 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0255, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000248 DAFNNB010000000
VERSION     DAFNNB010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0255"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..411)
                     /locus_tag="L3M60_003459"
     CDS             complement(<1..411)
                     /locus_tag="L3M60_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBN6228079.1"
                     /translation="MLEIIVPATSANIGPGFDCLGIALNIYNKFYVEEIENGLEIEGC
                     EDAYKNENNLVYTSMKYFFDRVKPEKIPTGIKIKIQSEVPICRGLGSSASCIVAGVIA
                     ANALSGANLDKNQLLNIASEIEGHPDNVAPAILGN"
     gene            complement(421..1902)
                     /locus_tag="L3M60_003460"
     CDS             complement(421..1902)
                     /locus_tag="L3M60_003460"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBN6228080.1"
                     /translation="MYYSSTRGTEEKVTASQAIIKGISNDGGLYVPSKFPNVKNELID
                     LVNLTYSQIAFFVLSKFLCDFTEDEIKNCIENAYDEKFDCSSIAPLNKVNDTYFLELY
                     HGPTLAFKDMALTIMPHLLKTSIKKDNLEKDVVILTATSGDTGKAALEGFKDIDKIKI
                     IVFFPEDGVSPVQKLQMKTQTGKNTYVVGIKGNFDDAQSGVKKIFSDKEFNQKLLDNN
                     YILSSANSINIGRLLPQVAYYFYSYMKLVDNGEINLNDKINFVVPTGNFGNILAGYYA
                     KQMGLPINKLICASNDNNVLYDFFKTGVYDKNRTLKLTTSPSMDILISSNLERLLFEI
                     SNRNTDIVNKLLSDLNDKGVYKISEDMEKNLASFHGEYSNEEEVKNTINSVFKNYNYL
                     IDTHTAVAYNCYEKYKKETSDNTKTVIVSTASPFKFSEDVLKSIDCDFKDLDDFAIID
                     RLSSISKIDIPKPIKNLKNAEILHKDIYEKDELKLAIKKFLKV"
     misc_binding    complement(2010..2240)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          943 a          393 c          305 g         1039 t
ORIGIN      
        1 atttcctaaa atagcaggag caacattatc tgggtgacct tctatttctg aagctatatt
       61 tagtaattga tttttatcta aattagcacc tgatagagca tttgcagcta taacgccagc
      121 tactatacaa gaggcactac taccaagacc tctacatata ggcacttcac tttggatttt
      181 tattttgata ccagtaggta ttttttctgg tttaactcta tcgaaaaagt acttcataga
      241 tgtataaact aagttatttt cgtttttgta agcgtcttca caaccttcta tttcaagacc
      301 attttcaatt tcttcaacat aaaatttatt gtatatattt aacgcaattc caagacaatc
      361 aaatcctgga cctatattag cacttgttgc aggaactata atctctaaca taataatcac
      421 ctataccttt aagaattttt ttatagctag ttttaattca tctttttcat aaatatcttt
      481 atgtagtatt tctgcatttt taagattttt aataggtttt ggtatatcta ttttagaaat
      541 acttgataat ctatcaatta tagcaaagtc atcaaggtct ttaaaatcac aatctataga
      601 ttttagaaca tcttcagaaa acttgaatgg acttgcagta cttacaataa cagtttttgt
      661 gttatcagaa gtttcttttt tatatttttc ataacaatta tatgcaacag cagtatgagt
      721 atctatcaaa taattataat ttttaaacac gctgtttatc gtgtttttaa cttcttcttc
      781 atttgaatat tctccatgga atgaggctaa atttttttcc atatcctcac ttattttgta
      841 aactccttta tcattaaggt ctgatagcag tttatttaca atatcagtat ttctatttga
      901 tatttcaaac agtaatcgct ctaaattact tgagattaat atatccatag aaggagaagt
      961 agttaatttc aatgttctat ttttatcata tacaccagtt ttaaagaaat cataaagaac
     1021 attattatca tttgatgcac atatcaattt gtttattgga agacccattt gtttggcata
     1081 atatccagct agaatattac caaaattacc agttgggaca acaaaattta ttttatcatt
     1141 taaatttatt tccccattat caactaattt catatatgaa taaaaatagt atgcaacttg
     1201 tggaagaagt cttcctatat taattgaatt tgcggaagat aatatataat tattatctaa
     1261 taatttttga ttaaattctt tatcagaaaa aatcttcttt acacctgatt gtgcatcatc
     1321 aaaatttcct tttataccaa ctacatatgt attttttcca gtttgagttt tcatttgaag
     1381 tttttgtact ggacttacac catcttctgg gaagaataca attattttta ttttatctat
     1441 atctttaaat ccctcaagtg cagctttgcc agtatctcca gatgtagcag taagaatcac
     1501 aacatctttc tctaagttat ctttctttat agatgtttta agaagatgag gcattatagt
     1561 aagtgccata tctttaaatg ctaaagttgg accatggtac agttccaaga aataagtatc
     1621 atttacttta tttaacggag ctatactact gcaatcaaac ttttcatcat atgcattttc
     1681 tatacaattt ttaatttcat cttcagtaaa atcacataaa aatttactaa gtacaaaaaa
     1741 tgctatctga gaatacgtta aatttactaa atctataagt tcatttttta cattgggaaa
     1801 tttacttgga acataaagac caccatcatt tgatattcct tttattattg cttgtgatgc
     1861 agttactttt tcttcggtgc ctcttgtact tgaataatac attttttata accctccata
     1921 tattttaaat ttttaattag ctaattaagt tagatacaga atattttaaa aactgaattt
     1981 aggtaataaa aaaagaccct catccctaag aaagggacga gaatctattt cccgcggttc
     2041 cacccaaatt gatttaaaaa aatccacttg atactttaac gtaagtaata aaacgtctat
     2101 tcctactaaa atcataaaga taacttcaga tagaagctca taggtagttt tcaatccttc
     2161 taattatgga aacatttcag ctagtagttt ccttctctgg taatcgattc agatttactc
     2221 ttcctaatca atgcctttca atattcttaa tattattaat tattctagtt tataaaacag
     2281 acaaagtcaa gtattaatat aaaaatttgg aaaaaatatt ttttaaattg taaaaaaatt
     2341 gtaatcaaat tgagtattag aaaaataatt ttttaacttt tttaaaagct taaaaaccta
     2401 gattttcata gtgatgaagc tgtttttagt aaaagtaatc aaaagataac tattaaatat
     2461 ctgtaatatt acaattttat attacactat attacaaaaa aagtttacaa aactttaatt
     2521 tgaaaaatgt aattatatgg tatatcatat aaatataagt aaatagtgct aatatttaaa
     2581 ctattaatca taattggttc aacaaaagta tatagttaat aatgtttaat ttgaagaaat
     2641 tgtcagtgta aaattacata tatattcaaa ttaaattcat
//
LOCUS       DAFNNB010000249         2680 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0256, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000249 DAFNNB010000000
VERSION     DAFNNB010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0256"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(295..1545)
                     /locus_tag="L3M60_003461"
     CDS             complement(295..1545)
                     /locus_tag="L3M60_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HBN6228081.1"
                     /translation="MATIKPFKAIRPNKYIVDKVAALPYDVMNSKEARRIAEGNPYSF
                     LHIDKSEIDLDENIDLYDEKVYLKARENLDEFRKQEILVKDDKECIYIYKQIMDGRAQ
                     VGIVACISVDESLNGTIKKHEYTRPEKEIDRTKHIKYCDANTGTILVTYKHQRVIDDI
                     INDFMNNNEPLYDFITDDKIEHTVWKIDNDDIICNLVDEFRKLDYLYIADGHHRTASA
                     ENVAKEMRAKNPNYTGKEEFNYFIAMIAPDENLMVLDYNRVIKDLNGLSEEEFINKIK
                     ENFELEEIEGKEKYKPDKKGTFGMYLCDKWYKMKANKNLLEIEDPVDSLDISILQDYV
                     IDAILGIDNPRVDKRIDFIGGIRGLEELEKRVNEDMKVAFAMYPTSIEDLIRVADANK
                     IMPAKSTWFEPKVRCGLFLHEINE"
     gene            complement(1577..2518)
                     /locus_tag="L3M60_003462"
     CDS             complement(1577..2518)
                     /locus_tag="L3M60_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003437082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HBN6228082.1"
                     /translation="MYNILVTDGIEKEAAKKLRELNFNVIEQFYEKDVLGDKLKDVDV
                     LVVRSATKVTKDVIDKALEGKKLKLIVRGGVGLDNIDVKYAQANGIKVMNTPNASSIS
                     VAELTIGQLFVLARFINTANVTMRDGKWEKKKYKGTEINGKTLGLIGFGRIAKEVAKR
                     AELLGMNVIYTDIMGEAQGFNNYKFCDMEEVLEKADFLSLHIPFDKNKGAVITEKEIN
                     KMKKGAYLINCARGGLVDEKDLLKALDEGKLSAAAIDVYEQEPTLNLDLVNHPRVSPT
                     PHIGASTVEAQERIGDEIVNVIQDFFLDFNNLIGVAL"
BASE COUNT          822 a          502 c          237 g         1119 t
ORIGIN      
        1 taaattttaa aaacactttt tttattaact tttttataat tataataaaa tatataacaa
       61 tactattata aatacacatt taaaaccttc caaatatata aatacaatta tataaatttt
      121 catcctcaat tataacatag aatatgacta tctatatgct aaaattatcc attttcaaat
      181 aaaggtaact attattaaaa atatcaactt ataggattta tatcccatat acgattttca
      241 atcaaaaaaa caagggctaa aaagtgaatt gtacactttt tagcccttta ttaattattc
      301 atttatttca tgtaagaata gcccacatct tactttaggt tcaaaccatg ttgatttagc
      361 tggcattatc ttatttgcat ctgctactct tattaaatct tcaatactag taggatacat
      421 tgcaaatgct actttcatat cttcattaac tcttttttct aattcttcaa gacctctaat
      481 cccaccaatg aaatcaattc ttttatctac tctaggattg tcaattccta aaatagcatc
      541 aataacatag tcttgtaata tagaaatatc taatgagtca acagggtctt ctatttctaa
      601 taaattttta tttgctttca ttttatacca cttatcacat aggtacattc cgaaagttcc
      661 ttttttgtca ggtttatatt tttcttttcc ttctatttct tctagttcaa aattttcttt
      721 tattttattt ataaattctt cctcgcttaa accatttaag tctttgataa ccctattata
      781 atccaaaacc attaaatttt catctggagc aatcattgct ataaagtaat taaattcttc
      841 ttttccagta taatttggat ttttagctct catttctttg gctacatttt ctgctgaagc
      901 tgtcctatga tgaccatctg caatgtatag ataatctaat tttctaaatt catctactag
      961 gttacatatt atatcatcat tatcaatctt ccatactgta tgctctatct tgtcatctgt
     1021 tataaaatca tataaaggtt cattattatt cataaaatca ttgattatat catctatcac
     1081 cctttgatgt ttataagtta caagtatagt tcccgtatta gcatcacagt attttatgtg
     1141 tttagttcta tcaatttcct tttctggtcg agtgtactca tgttttttta ttgttccatt
     1201 caagctttca tcaactgata tacatgctac tataccaact tgagctctac catccatgat
     1261 ttgtttatat atatatatac actctttatc atcttttact agaatttctt gttttcgaaa
     1321 ctcatctaaa ttttctcgcg cttttaaata aactttttca tcatataaat ctatattttc
     1381 atctaaatca atctctgatt tatctatatg caaaaatgaa tatggattcc cttctgcaat
     1441 tcttctagct tctttactat tcataacatc gtaaggaagt gctgctactt tatctactat
     1501 atatttattt ggtctaatcg ccttaaatgg tttaatcgtt gccattacta atttcctcct
     1561 aattatacaa aatattttat aatgctaccc ctattagatt gttgaagtca agaaagaaat
     1621 cttgaatcac atttactatt tcgtctccta ttctttcttg agcttctact gtagatgccc
     1681 caatatgagg tgtaggagaa actcttggat gatttactaa gtctagattt aatgtaggtt
     1741 cttgttcata aacatctata gcagcagctg ataatttacc ttcatctaat gcttttagta
     1801 aatctttttc atcaactaaa ccacctcttg cacagtttat taaataagcc cctttcttca
     1861 tcttatttat ttctttttca gttattactg cccccttatt cttatcaaat ggtatgtgta
     1921 aagataagaa atctgccttt tctaaaactt cttccatatc acagaattta taattgttaa
     1981 agccttgcgc ttcacccatt atgtcagtat atataacatt catacctaat aattctgctc
     2041 tctttgcaac ttcttttgca atccttccaa aacctataag tccaagtgtc tttccattaa
     2101 tttctgtacc tttatatttt ttcttctccc attttccatc tctcatagtt acatttgcag
     2161 tatttatgaa tcttgcaaga acaaataatt gacctattgt aagctctgca acagatatac
     2221 tgcttgcatt tggagtattc ataactttta tgccattagc ttgagcatat ttaacgtcta
     2281 tattatctag tcctactcct cctcttacta tcaacttcaa ctttttaccc tctaatgctt
     2341 tatctattac atctttagta accttagtag ctgaacgtac tacaagtaca tcaacatctt
     2401 taagtttatc tcctaaaaca tctttttcat aaaactgctc tatcacatta aaatttaact
     2461 ctctcaattt tttcgctgct tctttctcta ttccatcagt aactaatata ttatacataa
     2521 caaactcctc caatttttat tcacttaatt tttccaattt ttattttttt aagtattttc
     2581 atcaataaaa cactttactt aatttaactt tctgtttata taattttcaa aatatttttt
     2641 tattaaaatc atatcaaatt ttcctattgt ttgttaaaaa
//
LOCUS       DAFNNB010000250         2679 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0257, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000250 DAFNNB010000000
VERSION     DAFNNB010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0257"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(207..914)
                     /locus_tag="L3M60_003463"
     CDS             complement(207..914)
                     /locus_tag="L3M60_003463"
                     /inference="COORDINATES: protein motif:HMM:NF019884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6228083.1"
                     /translation="MSLEEAKELEKNYKLPMPTFCLKEKGYFAREYAALMLLSNGGMK
                     IMHTEKNRYLYQNKFDIDALAKQLSISRATLYRNIKKLEELKCGILKIENTKNGTVYR
                     LKYGIETGYNDEVHKFVTIHHKILEELVINFKTPSVKLYCLLCYLTNEIDFKVIDEEF
                     LCNNIGLCGKSESNKNKVRSMIRVLMKCKYIDVKKENRTFWNEETKNKINKTLKLYRL
                     CSYEEWKNLNDKTDFKD"
     gene            1407..1559
                     /locus_tag="L3M60_003464"
     CDS             1407..1559
                     /locus_tag="L3M60_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228084.1"
                     /translation="MDNFLLNILAGVIASLIFYIISKLFRQVKNHSAQKSGWEFDLKI
                     KFKKSR"
     gene            2380..2562
                     /locus_tag="L3M60_003465"
     CDS             2380..2562
                     /locus_tag="L3M60_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HBN6228085.1"
                     /translation="MKKEKRDLKFNFHKGGSGSFTPRMSVPKKWADEMGITRENPNVT
                     ATFEGDRIIIEKAKNE"
BASE COUNT          958 a          347 c          333 g         1041 t
ORIGIN      
        1 aagaaataaa tagaaaacat atttatgttt tctatttatt aagttctcta tatatttaga
       61 ccaccacttt ttgagaatgc gaccaccact ttttgagaat gcacccacca ctttttgaga
      121 caagttagat ttctaattaa aaaacaataa gttgtccaca ttttatcaac aggttattaa
      181 catagttatc cacaaaaaaa cagaccttaa tctttaaagt ctgttttatc atttaagttt
      241 ttccactctt cgtaactaca taatctatat aattttagtg ttttattaat tttatttttt
      301 gtttcttcat tccagaaagt tctattctct ttttttacgt ctatgtattt gcatttcatt
      361 aatactcgta tcatacttct aactttgttt ttattacttt cgctcttgcc acaaagacca
      421 atattattac ataaaaattc ttcgtctata actttaaaat ctatttcatt tgttaaataa
      481 cataaaagac aataaagttt tacgcttgga gttttgaaat tgattactaa ttcttcaagt
      541 attttatgat gtattgtaac aaacttatgc acttcgtcat tataacctgt ttcaattcca
      601 tattttagtc tgtatacagt tccatttttc gtattttcta tttttagaat accacattta
      661 agttcttcta attttttaat atttctgtat aatgtagctc ttgaaatgct tagctgtttt
      721 gctaatgcat ctatatcgaa tttattttga tataagtatc tatttttttc tgtatgcata
      781 attttcattc ctccgttact taataacatc aaggcggcgt attctcttgc aaaatatcct
      841 ttttctttca aacaaaaagt tggcattggt aatttataat ttttttctaa ttcttttgct
      901 tcttctaaag acattttatt tcccccttct aaacggggct tgaagcactt ttaactaatt
      961 aagttaaaaa tttcaaatta tttttaactc taaaacattg atttataagt tgttttaatc
     1021 tataatataa cttataaatt agttaaaaaa agatataatt atatcttata agagcttcaa
     1081 gctccgtggt aaggaataag tgttagtttg gcgacttggt gcttattcct tttttatttg
     1141 tttttgtaat ctgtaataag ttaatattaa ttatagcata aatataaaat aaaaaaatat
     1201 attgattttg aaaataaatt atattttaaa aatatatttt ctaaaaaact gtattttaaa
     1261 agacttagta cacttactaa gtcttttttt atgtaaatat atgttataat aattttagca
     1321 agagaagcaa tctatattct aaaaaaagag tggagtctat acacaaaaga ttatcctccc
     1381 aacgctatga ggggaggtga atatgtatgg ataatttttt acttaacata ttagctggtg
     1441 ttatagctag tttaatattc tatataatta gtaaattatt tagacaagta aaaaaccact
     1501 ctgcgcaaaa gagtggttgg gaatttgatt taaaaatcaa attcaaaaag tctagataat
     1561 ttttttactt tagacgaaaa ctctactctt acgactagaa tacagttctc ttgcttttat
     1621 tatatcacat tttctttaaa agatacaact ttacatcatt ttcagtattc tattttttta
     1681 tttttatcaa agtttatttt aagaacgata aaattaaaaa acactcaaac gccaataaga
     1741 gtggtttaag aaaataagtt atttttaatc tgattggaaa tagctactct tgtgtaaagt
     1801 aaatcattat ttccttgctt ttattatatc acatttttta aaaaagatac aattttacat
     1861 catttctaat atactaaaat ctttactttt taccaaaatt tgtttaaaaa ataggagaaa
     1921 actattgcct ttgacgcccc gtcatgatat aatatattta tagagaagga ggtgagaaag
     1981 taaaaagaaa taaaagaaag gaggcacagg aatggggaga ttagaaagaa gtaaaaagaa
     2041 aagagaaaac aaatttaaca tagttaaaaa agttttctcc ttcatcttat tattacttaa
     2101 cataatcctt gcaattctaa gaattttaaa agagttgtaa ggataagcct agagagaata
     2161 gcccttctct ctaggtgact tctttctaat attataacac atttctaaaa aaacatgagt
     2221 gataaatttt ataagatttg tattgtttta gttttaataa ctatagtttt aaatatagta
     2281 tctatagtat taaactctag tacaagcaac atactcggtt tagtttttag tgttgtatta
     2341 ttacttttct ttgtaataca gcaaaaaagg agctaatgaa tgaaaaaaga aaaaagagat
     2401 ttaaagttta actttcataa aggtgggagt gggtcgttta ctcctagaat gagtgttccc
     2461 aaaaaatggg ctgatgaaat gggcataaca agagaaaacc ctaatgttac ggcaacattc
     2521 gagggcgaca ggataataat tgaaaaggct aagaatgaat gactttttct ataaaaagaa
     2581 ggttgctttt ttcttagcct tcttttttaa attttactat aatataaaaa taatactgta
     2641 atattataaa aatattattt tagtattgca tatacaata
//
LOCUS       DAFNNB010000251         2562 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0259, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000251 DAFNNB010000000
VERSION     DAFNNB010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0259"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            54..1055
                     /locus_tag="L3M60_003466"
                     /pseudo
     CDS             54..1055
                     /locus_tag="L3M60_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426589.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(1241..2095)
                     /locus_tag="L3M60_003467"
     CDS             complement(1241..2095)
                     /locus_tag="L3M60_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228086.1"
                     /translation="MSTFYIQFLYFVIYSFIGWCCETTYCSVLQKQFVNRGFLNGPFC
                     PIYGFGALAIVATLTPFVHNIPLLFLFSIIITSIMEYCTSFILEKIFNMTWWDYSKHK
                     FNIHGRVCLENSLMFGVLSLIVMLIVHPIVVDFINSISKNVLFIFAISIEVYFILDLV
                     ITVHTILQLNGKLKQINLIIKELKDKKEYYKFITQETIENKLESLVENRLDTLEDRLE
                     ILKEKENYRYAKNRIDELKNKLNTLLSNHKLLHRRIIKAFPNISSNKHTDILNKIKEN
                     IKSIKKDK"
     gene            complement(2359..>2562)
                     /locus_tag="L3M60_003468"
     CDS             complement(2359..>2562)
                     /locus_tag="L3M60_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1540 domain-containing protein"
                     /protein_id="HBN6228087.1"
                     /translation="SFLDESIYSNLSSNVNNRNCEELVCTVKNCKYNKDGSLCTLDNI
                     LVAPETDRVNLYIETCCSSFECK"
BASE COUNT          976 a          313 c          387 g          886 t
ORIGIN      
        1 agtatgcaaa attctgtgaa gcaggaaccc tgtgacttta gtcatgggag gttcagggtg
       61 aaataataat gccatttttt aaagaaggtg gtaggtttac tatagataat gtacattatg
      121 ttaaagaagg agaaatactt gtaccagcag gaatgactga atttgcaaag gataaatctt
      181 ttggatacaa atcatcccac ataggtgagt ggtgtgaaga aaagacaaat ggaaagtaca
      241 aatctagtga tatgatatat atttcttctg aagaacttag agaactaaat attgaagcca
      301 taacagaaaa acttaaaatg gctgaaggat tcaataaaat tattgtaaat gcaaccgact
      361 atgtagatgt aaaggtattt acaattgcat ttatacgagc agtaaatagt ggtaaggaat
      421 ttatgtttag aagcgctgct gccataacaa aagtacttgg tggagtgagt gataaggaac
      481 tgcttactaa ggatgagtta gtgtcaaaag gtaatactaa tggtggaatt atacttgttg
      541 gttcacatgt aaataagact acccaacagt tagaagaact taaaaattgt aaatatccaa
      601 tagaatttat tgaatttaat caacacttag ttcttcagga aaatggtcta aaaaatgagg
      661 taaaaagagt tataaaagtt gtagagaaaa aaataagcag tggtaaaact gttgctgttt
      721 atactagacg agagcgtttt gacctggata cgaatgataa agataaacag cttatgattt
      781 ctgtagaaat atcagatgca gttaccagta taattggtat gcttaatgta agacccaact
      841 ttattatagc taaaggtgga attacttcta gtgatgttgg cactaaagca ctaagagtaa
      901 aaaaagctac agtaatggga caaataaagc ctggtatacc agtatggatg acaggcaaag
      961 aaagtaaatt tcctaatatg ccatatataa ttttccctgg aaatgtggga gagattagca
     1021 cacttaggga atcagttgag atattgatgg gatagcatat cagtgtattg aagtttaaac
     1081 actcagattt actgagttaa aagtgaaata aactaaaatt aaaaattaag attaaaaata
     1141 atagtatatt aattaaggtt tagagtattt tacaaaaaat attctaaacc ttaatcttat
     1201 gtattagtat ttttattatt gaacttatgt gtttatttga ttatttatct ttttttattg
     1261 atttaatatt ttctttaatt ttatttaaaa tatctgtatg tttattagag cttatattgg
     1321 ggaatgcttt gattatacgt ctatgaagta atttgtggtt agacagcaaa gtattaagtt
     1381 tattttttag ctcatcaata cgatttttag catagcgata attttctttt tccttaagta
     1441 tttcaagcct atcttctaaa gtatccagtc tattttcaac taaagattca agtttgtttt
     1501 caatagtttc ttgtgtaata aatttgtagt attctttctt atcttttaat tccttaatta
     1561 taagattgat ttgttttaac ttaccattta attgtaaaat tgtatggact gtaattacta
     1621 aatctaaaat aaaataaact tctatagata tagcaaatat aaatagcaca tttttagaga
     1681 tagagtttat aaaatctact acaataggat gtactataag cattacaatc agtgataata
     1741 caccaaacat aagtgagttt tccagacaaa ctcttccatg aatattgaat ttgtgtttag
     1801 aataatccca ccaagtcata ttaaatattt tttctaaaat aaagctcgta caatactcca
     1861 ttatgctagt tataattata ctaaacaaaa ataacaaagg aatattgtgt acaaaaggag
     1921 tcaaagtagc aactattgct aatgctccaa atccatatat aggacagaat ggaccattta
     1981 aaaaacctct gtttacaaat tgtttttgta aaactgaaca atatgtagtt tcacaacacc
     2041 atcctataaa actataaatt acaaaataaa gaaattgaat ataaaatgta ctcataagca
     2101 cctcatttct agttagtata acactatata ctcgatattt tatttttatg attaatttac
     2161 taaattcaat ttacatattg attatatcaa tttatagaaa tttatcaaat ttaattacag
     2221 ttagaacatt ataaacatgt ttataataat aaaattttat ttaaaataag ggtgtcaagt
     2281 ataatcaaac taatagttgt agatactcta cactatgtta gtttaattaa atttgacacc
     2341 cttatattta tactatgact atttacattc gaaactagag caacatgttt caatataaag
     2401 gttcactcta tcagtttcag gagcaaccaa tatattatca agtgtacata aactaccatc
     2461 tttattgtat ttacaatttt ttacagtaca aactaattcc tcacaatttc tattattaac
     2521 attgctagat agattgctat aaatagattc atctaaaaat ga
//
LOCUS       DAFNNB010000252         2497 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0262, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000252 DAFNNB010000000
VERSION     DAFNNB010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0262"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..454
                     /locus_tag="L3M60_003469"
     CDS             <1..454
                     /locus_tag="L3M60_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBN6228088.1"
                     /translation="IHYDIIKIPEELKFCAMIPNFKLSTEKARGVLPKEIPYSDGVFN
                     VSRVALLVSALINKNFELLKVACQDKLHQDYRGTLIENYNDIVEKSEQLNSIGVFLSG
                     AGPTIMSLIRENDDSFVDNMRNYLQNLKSDWEIKELYCDSNGAVLNIL"
     gene            578..1639
                     /locus_tag="L3M60_003470"
     CDS             578..1639
                     /locus_tag="L3M60_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HBN6228089.1"
                     /translation="MIIKELIIRDLVAKVPIIQGGMGVGISRSSLAGAVAKLGGVGVI
                     SGVQIGYDEEDFETNTINANLRAIKKHISKAKEISNGGIIGINFMVAMKEYETYVKEA
                     VKAGVDLIISGAGLPNKLPSLVKGSNVKIAPIVSTAKAANVILKMWDRKEKTTADLIV
                     VEGPKAGGHLGYSNEELDNIDSIDYDKEFVEILKVANTYGEKFGKKIPVVAAGGITSS
                     SDVKKYIDMGASGVQVGTRFVATYECDAHENFKMAYINASEEDVHIVKSPVGLPGRAI
                     RNKFIEEVKINRPEIKKCYNCLIPCNPKETPYCISQALINAVKGDVENSLLFCGNDAY
                     KIDKLSTVEDVINELISEL"
     gene            complement(1793..2305)
                     /locus_tag="L3M60_003471"
     CDS             complement(1793..2305)
                     /locus_tag="L3M60_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBN6228090.1"
                     /translation="MSDFKEILAEEIKSNPFGLIGKDWTLITAENEGKVNTMTASWGG
                     LGVMWGKDVAFVVIRPQRYTKEFIDNTDKFSLTFFDEDFRKELSYCGKVSGREEDKIS
                     QVGFNIEHLNNTPYFKEAKMALICKKMYSQKLEPQCFIAEGIDGRWYPQKDYHTLYIA
                     EITNVLVKEA"
BASE COUNT          893 a          332 c          383 g          889 t
ORIGIN      
        1 tatacactat gatattataa aaattcctga agaacttaaa ttttgtgcaa tgatacctaa
       61 tttcaaatta tcaacagaaa aagctagagg tgttttacca aaggaaatac catattctga
      121 tggggtattc aatgttagta gagttgcttt actagtatct gctttaataa acaaaaactt
      181 tgaacttctt aaagtagctt gtcaagacaa attgcatcaa gattatagag gaactctaat
      241 tgaaaactac aatgatattg ttgaaaaatc tgaacaatta aatagtattg gagtattttt
      301 aagtggagct ggacctacta taatgtcttt aataagagaa aatgatgata gttttgtaga
      361 taatatgaga aattatctac aaaacttaaa atctgattgg gaaataaaag agttatattg
      421 cgattctaat ggagctgttt taaatatatt ataattatcg tcttctatac aaaatataac
      481 tactctttta ttataatttg tagtataata attaggacta ggtaataata ttaagtatta
      541 taatctagtt ctttaaacat gtttacattg aggagggatt atcataaaag agttaataat
      601 tagagattta gttgcaaaag ttcctataat tcaaggtgga atgggcgttg gtatatctag
      661 gtcatcacta gctggtgcag ttgctaagct tggtggtgtt ggtgtaattt ctggagttca
      721 aattggatat gatgaggaag attttgaaac taatactata aatgcaaatt taagagctat
      781 aaaaaaacat atttctaaag ctaaagaaat ctcaaatggc ggtattattg gtattaattt
      841 tatggtagct atgaaagaat atgaaacata cgtaaaggaa gctgttaaag ctggtgtaga
      901 ccttataata tccggagctg gattacctaa caagttacca tcattagtaa agggaagtaa
      961 tgtaaaaata gcacctatag tttcaactgc aaaagctgcc aatgtaattt taaaaatgtg
     1021 ggatagaaaa gagaaaacta ctgctgactt aatagttgtt gaaggtccaa aagctggtgg
     1081 tcaccttgga tactctaacg aggaacttga taacatagat tctatagact atgataagga
     1141 atttgttgaa attttaaaag ttgcaaatac ttatggagaa aaatttggta aaaaaatacc
     1201 agtagttgct gctggtggta taacttctag tagtgatgtt aaaaaatata tagacatggg
     1261 tgcaagtggt gtacaagttg gtacaagatt tgttgctact tatgaatgtg atgcccatga
     1321 gaactttaaa atggcataca taaatgctag tgaagaagat gttcatatag ttaaaagtcc
     1381 tgtaggtctt cctggacgtg ccataagaaa taaatttata gaagaagtta aaataaatcg
     1441 tccagaaata aagaagtgct ataattgttt aattccttgt aatccaaaag aaacacctta
     1501 ctgtatttct caagctctaa tcaatgcagt taaaggagat gttgaaaatt cacttttatt
     1561 ttgtggaaat gatgcctata aaattgacaa attaagcact gttgaagacg ttatcaatga
     1621 attaatttct gaattataat ttaaagaaat ctaaattaaa attttatcaa gctaaggagt
     1681 ttaaatacgt tatttattaa tctgtatcaa catatttact tccttagctt ttttgtgcca
     1741 atttcctttt atagagattt tatttaatta gagatagtat taacctttat aactaagctt
     1801 cttttacaag tacattggtt atttcggcta tatataaagt gtgataatct ttttgtggat
     1861 accatcttcc atctatacct tcagcaataa aacattgagg ttccagtttc tgactataca
     1921 tttttttaca tataagagcc attttcgctt ctttaaaata aggtgtatta tttaagtgtt
     1981 ctatattaaa tccaacctgt gatatcttat cttcttctct accagatact tttccacaat
     2041 atgaaagctc tttacgaaaa tcttcatcaa aaaaggtaag agaaaattta tctgtattat
     2101 ctataaattc cttagtataa cgctgtggtc ttattactac aaaagcaaca tcttttcccc
     2161 acataactcc aagccctccc cagctagcag tcattgtgtt tactttgcct tcattttctg
     2221 ctgttataag tgtccaatct ttacctatca atccaaatgg attactttta atctcttctg
     2281 ccaaaatctc tttaaaatca ctcatatctc ttataacctc tcattctctt atatttatat
     2341 tccttttatt ttcgtgataa attataaatg aagtctagtt ttgaaaataa aataaatttt
     2401 tttaaatata ttattacaaa taagtaaata tatagataca ctcttatttt ttaatatatt
     2461 taataatttc actcagaata tatatattct ttattca
//
LOCUS       DAFNNB010000253         2371 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0265, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000253 DAFNNB010000000
VERSION     DAFNNB010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0265"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..2071
                     /locus_tag="L3M60_003472"
     CDS             <1..2071
                     /locus_tag="L3M60_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228091.1"
                     /translation="KNYKVVGILKDRPYYKSIGMAELILPFTENIIKETNLEAQIRFK
                     KDIYRNLSKLMNDNHISRKNVSENRILLDALGESNGLNYDFIFTCIISMIVSGVVIYS
                     IFRISIIQRISEYGIIRALGANIFNVFNIIFVELFAISLVSIPIGVLIGIISSKYITL
                     FMGNLLIGNIVKINNVEINLDVVMISIITVLVNILIISFLTCTNVNKISPIKCIKKDI
                     SKKNSRESSILNTRRLLNLLPIEYIISLKNILKNKKNFVIIVLSMSGGCIIFILTNFT
                     NILDERQLEYTSKVATENSDYNISFAPVTPLNIGISEENLKQIRQLEGIENVKTSQVM
                     YSRMILNKDNINNMNYFEKVNKSAYNKKALNGLLVKKDNEYILKSNLYGYDNSSIDAQ
                     KSFVISGDIDLDKMNNGNVAILYMPDPSLFTNIKDSRPVLNLKVGDIVKLRFRSDYVS
                     DENFAKLKDKGEYVEEEFKIGAIVSNILNVDGYYAGMDSASIIISQNQFKNIVGIHSY
                     RMLDIKKIPTVDNIELGKNIIDISSKIQGSIVRDFTQEIKNIKIIEDNKKILIYTIIL
                     VLFTISFINIINNINYNLYSRINEFGVIRAIGLDNNSFRKMLLFEGVVYGLISASVSI
                     TFGILGQSILYNILKGNLMNAKFIIDYKSYILVIILTIILGVVATYLPSKKVKKLSII
                     ESINIIE"
BASE COUNT         1005 a          196 c          334 g          836 t
ORIGIN      
        1 taaaaattat aaggtagtag gtatactaaa agatagacca tattacaaat ctataggaat
       61 ggcggaacta atacttcctt ttacagaaaa tataatcaaa gaaacgaatc tagaagctca
      121 aataagattt aaaaaagata tatatagaaa cttatcaaaa ctaatgaatg ataatcatat
      181 aagtagaaaa aatgtatctg aaaatagaat actattagat gccttaggag aaagtaatgg
      241 actaaattac gattttatat ttacatgcat tatatcaatg attgtatctg gagtagtaat
      301 ttattctata tttagaatat caataattca aagaatttcg gaatatggaa ttattagagc
      361 cttaggagca aatatattca atgtatttaa tataatattt gtagagcttt ttgcaatttc
      421 tttagtatct attccaattg gagttttgat aggtattata agttctaaat atataacctt
      481 atttatggga aatttactta ttggcaatat agtaaaaata aataatgtag aaataaattt
      541 agatgtagtg atgatatcta taataacagt acttgtaaat atattaatta taagtttttt
      601 aacatgtaca aatgtaaata aaatatcacc tataaaatgt ataaagaaag atatatctaa
      661 aaaaaattct agagaatcaa gtatattaaa tactaggaga ttactgaatc ttttacccat
      721 agaatatata atatccttaa aaaatatttt aaaaaataaa aaaaattttg tgattatagt
      781 attgtctatg agtggagggt gtattatatt tatattgact aattttacaa acatattgga
      841 tgaaagacaa ctagaatata catctaaagt tgcaacagaa aattcagact ataatataag
      901 ctttgcacct gttactccat tgaatatagg tatatctgaa gaaaatttaa aacaaattag
      961 acaactagaa ggcattgaaa atgttaaaac atctcaagtt atgtattcaa gaatgatatt
     1021 aaataaagat aatataaata atatgaatta ttttgaaaaa gtaaataagt ctgcttataa
     1081 taagaaggct ttaaatggat tattagttaa aaaagataac gagtatatat taaagagcaa
     1141 tctatatggt tatgataatt catcaataga tgctcaaaaa tcctttgtta tttcaggaga
     1201 tatagattta gataaaatga ataatggaaa tgtggcaata ctttatatgc cggatcctag
     1261 tttatttaca aacattaaag attctcgacc agttcttaat ttaaaggtag gagatattgt
     1321 aaaattgagg tttagaagtg attatgtatc agatgaaaat tttgcaaaat tgaaagataa
     1381 aggagaatat gttgaggaag aatttaaaat aggtgcaata gttagtaata ttttaaatgt
     1441 agatggttat tatgcaggta tggacagtgc gtctataata atatctcaaa atcaatttaa
     1501 aaatattgta ggtatacata gttatagaat gttagatata aaaaaaatac caacagttga
     1561 caatatagag cttggaaaaa acataattga catatctagt aaaattcaag gttcgatagt
     1621 tagagacttt acacaagaga taaaaaatat aaaaattata gaagataata aaaaaattct
     1681 tatatacaca ataatattag tcctttttac aattagtttt ataaatataa taaataatat
     1741 aaattataac ttatactcta gaataaatga gtttggtgta ataagagcta ttggattgga
     1801 taataatagt tttagaaaga tgctattatt tgaaggtgtt gtttatggac ttatatctgc
     1861 tagtgtgtct attacctttg gaattttagg tcaatctata ctctacaata ttttaaaagg
     1921 taatttaatg aatgccaaat ttataataga ttataaatca tacatattgg taataatttt
     1981 aactattatt ctgggagtag tggcaacata tttaccatct aaaaaagtta agaaattatc
     2041 tattattgaa tctataaata ttattgaata aaatcataag taaaatacat ttatgctgtg
     2101 taaatagtaa ttactaggaa ataatataaa aaataaataa aaatgataaa gtaagtatta
     2161 tatttattat atattatggc cttataatgt ttttattaat attaatagga aatttatctt
     2221 atatacatga aattagtatt tacaaaatta aaatgttgta aagtgtatgt atttatttta
     2281 tattatgggg tcagatgttt taataacaga ggcaatttat tcaagattta tgtaacttaa
     2341 tttagaatgt tatttaaaag aaaatctttt g
//
LOCUS       DAFNNB010000254         2321 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0267, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000254 DAFNNB010000000
VERSION     DAFNNB010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0267"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            162..2207
                     /locus_tag="L3M60_003473"
     CDS             162..2207
                     /locus_tag="L3M60_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HBN6228092.1"
                     /translation="MKKHNILFVSTDDKINIDISKQLENIFGEFCSIDNLIYVNRINI
                     ELSSYELVVCSDNDIKEYIHNNIDKNIPIVIIHRTINIENINQIISIENDSDVMVIDA
                     YKESADETAKIIRKLGLIHINLIPYYPGCDKSKCEIGIITGSRNSIPQNIKQIIDIGD
                     KIIDINTVIEIFTKLNISIDKLHIIKEKYDEDTVSGYRYYTTMNKTMKSFLEIIDEGI
                     ASIDKLGKFIYCNKVFSNLIGIDQNEIISNNFMDLFSDKVVKKIFFQEDEVNDEVVNL
                     NNKKLIINKVNVYENNERIKSIISIKDISAIQVLEDKIQNKFQAKGFVSKYTFESVVG
                     ESKIIKEKINIARKIAITDFSVLILGENGTGKEIFAQAIHNESLRKNKPFVAVNLSSL
                     SDTLIESELFGYEEGSFTGAIKGGKMGIFERAHTGTIFLDEIGDISLDVQQRLLRVLQ
                     EKEVMRMGGSKIIPIDVRIIAATNKDLKKKIKEGSFREDLYYRINVLHIEIPRLRERK
                     EDISLISKYFLDEINSNKCFTEESMKALKLYEWPGNVRELKNLVYYIDTIVEEDRVDY
                     EHLPEQFRFEKNNTLVNENFDSIILDFKQSNFFEESICILTSVETWNNKNILLGRNKL
                     QEILKEKGIVLSVDQIRKRIDKLKSHGLLLSGVKKQGSFITDEGKNFISYIKFKGVI"
BASE COUNT         1018 a          197 c          385 g          721 t
ORIGIN      
        1 aatttaaatt atatgtgaaa aaagataatc tacattattt tattgtggaa aataatagaa
       61 aatggtacaa aatttgattt ttaagattta aaactttttt aaaaagtgct acaatagacc
      121 aaattattac tttctatact cattaaacag gaggtgggac tatgaaaaaa cataatattt
      181 tatttgtatc aactgatgat aaaataaata tagatatatc aaaacaattg gaaaatatat
      241 ttggagaatt ttgtagtata gataacttga tatatgtaaa taggataaat attgaactat
      301 caagttatga acttgtagta tgctcagaca atgatataaa ggaatatata cataataata
      361 ttgataaaaa tatacctatt gtaataatac atcgtactat aaatattgaa aatataaatc
      421 agataataag tatagaaaat gattctgatg ttatggttat agatgcttat aaagagtcag
      481 cagatgaaac tgccaaaata attagaaaac taggattaat tcacattaat ctaataccat
      541 attatccagg ttgtgataag tctaaatgtg agattggaat tattacagga agcagaaaca
      601 gcataccaca aaacataaag caaataattg acataggcga taaaataatc gatataaata
      661 cagttataga gatttttaca aaactaaata tatctataga taaattacat attattaaag
      721 aaaaatatga tgaagataca gtaagtggat acaggtatta cacaactatg aataaaacca
      781 tgaagagttt tttggagatt atagatgaag gtattgcttc tattgataaa ttgggaaaat
      841 ttatatattg caacaaagta ttctctaatc taattggaat agaccaaaat gaaattatat
      901 ctaataattt tatggattta tttagtgaca aggttgtcaa aaaaatattt tttcaagagg
      961 atgaagttaa tgatgaagta gtaaatttaa ataacaaaaa actgataatt aataaagtta
     1021 atgtttatga aaataatgaa cgaataaaga gtattataag tattaaagat ataagtgcaa
     1081 ttcaagtact tgaggacaag attcaaaata aatttcaagc aaaaggtttt gtatcaaagt
     1141 acacatttga gagtgttgta ggtgagagca aaataataaa agaaaaaatt aatattgcaa
     1201 gaaaaatagc aattacagac ttttctgtgt tgatacttgg agaaaatgga acaggaaaag
     1261 aaatatttgc ccaagcaata cataatgaat ctctaagaaa aaacaagcca ttcgttgcag
     1321 ttaatctatc ttctttatca gatacactta ttgaaagtga actttttggt tatgaggaag
     1381 gaagttttac aggagcaata aaaggtggga aaatgggtat atttgaaaga gcacatacag
     1441 gaacaatttt tttagatgag ataggtgata tatctttaga tgtacagcaa agattgctaa
     1501 gggtattaca agaaaaagaa gttatgagaa tgggtggaag taaaattata ccaatagatg
     1561 ttagaattat agctgcaaca aataaagatt taaagaaaaa aattaaagaa ggttctttta
     1621 gagaggattt gtattatagg attaatgtat tacatataga aatacctaga cttagagagc
     1681 gtaaggaaga tatatctctt ataagtaagt attttttaga tgaaataaat agcaacaaat
     1741 gttttacgga agaatcaatg aaggctttaa aattgtatga atggccagga aatgtaagag
     1801 agcttaaaaa tttagtttat tatattgata ctatagttga ggaagataga gtagattatg
     1861 agcatttgcc agaacaattt agatttgaaa aaaacaatac tttagttaat gaaaattttg
     1921 actcaattat cctagatttc aaacaatcga atttctttga ggaaagtata tgtatattga
     1981 ctagtgtaga aacttggaat aataaaaata tattattagg aagaaataaa ctgcaagaaa
     2041 ttttaaaaga aaaaggtata gttttaagtg tagaccaaat tagaaaaaga atagataaat
     2101 taaagagtca tggtttactt ttatctggag taaaaaaaca aggtagtttt ataactgatg
     2161 agggcaagaa ctttatatca tatataaaat ttaagggagt gatatagggg gctaaaaagg
     2221 caaaataccc taaaagaatc cctatagtag atagaaataa ataaaattat ttttaagatt
     2281 aagataattc aaaaatattg aataaataat ctaaaaagat t
//
LOCUS       DAFNNB010000255         2242 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0270, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000255 DAFNNB010000000
VERSION     DAFNNB010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0270"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            268..>2242
                     /locus_tag="L3M60_003474"
     CDS             268..>2242
                     /locus_tag="L3M60_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein CwpV"
                     /protein_id="HBN6228093.1"
                     /translation="MKIPKKIAAMLTVTMIAGSSTAGIASAQTVATNLTGQERYETAV
                     KISQDGWKNADEVVIVNDSSIADALSATPFAKAKNAPILLTGKDKLNDKTKAEIQRLK
                     AKKVYLIGGTSVLSTNIEKEIKDLKISFERISGAERYQTSLELAKKLDAISDVKKIAV
                     VNGEKGLADAVSVGAPAAQNNMPVILADSKNGTAVADKFIKDAGITQSYVVGGESSIS
                     EAVKNKLPNSTRLGGTDRNDTNAKVIKEFYKKTDLKNAYVTKDGMNKQDQLIDALAVG
                     VLGAKNQSPVVLVGKNLSASQKSLVNSKSFDKITKVGGNGNETAFNEMKSLQEVKTVE
                     AKTISELKSAIDKATANDVINFKPTSEVKEAFTIQTDKAVTVNLNGTYTKTVTINMPN
                     GDVNNYAKVDDVVIDDVKDGTFVNYGKITNLKVNDKNGAKIENNSKGEIGSLTVASGA
                     SQVKVTNGGKITTVTNNSKGTTIDNKGTISSVKGDNSPTISGNSPSSNSSGGSSSSGG
                     SSHGGGSGGSSSSKVGQIKFNATDKQIKMASGDKTVDPSDNTYVLKLTNGTVKDGDVK
                     ANVEVTGLPSGLDYTAVGNKSANTITITVSGTASQALQADLDNVSVLVKAGAVSETTA
                     TDSIANATFEVKKYVAAPAVGQLTFDATDKQIKM"
BASE COUNT          965 a          287 c          414 g          576 t
ORIGIN      
        1 tgtgaaaccc tactttttac aacaattcat taacataaaa atcaaactta ccttaaaata
       61 taaatttaca aatttttcaa tcaataaaaa aatttcttta ttccttatat aatgactgca
      121 atgtatcaaa ataattacaa attaattatg aaatcattat agttagaaac ctctttaaaa
      181 ttagatatta agtatccttt agaattagaa cgggaacaaa gttaaaaaat taagtaatat
      241 caaatggaca agggggaaga aaatacaatg aaaataccaa agaagattgc tgcaatgctt
      301 acagtaacaa tgatagctgg aagttcaaca gcaggcatag ctagtgcaca aactgtggca
      361 acaaatttaa cagggcaaga aagatatgaa actgctgtta aaataagtca agatggatgg
      421 aaaaatgctg atgaggtagt aatagtaaac gactcatcta tagcagatgc tctatctgca
      481 acaccatttg caaaggcaaa aaatgctcca atacttttaa caggtaaaga taaattaaat
      541 gataagacga aagcagaaat acaaagatta aaagctaaaa aagtatactt aataggtgga
      601 acttctgtat taagcacaaa catagaaaaa gaaataaaag atttaaaaat atcttttgaa
      661 agaatctctg gagctgagag atatcaaaca tccttagaat tggcaaagaa attagatgct
      721 ataagtgatg ttaagaaaat agctgtagtt aatggtgaaa aaggacttgc agatgctgta
      781 agtgtaggag caccagctgc acaaaacaat atgccagtaa tacttgctga ttctaaaaat
      841 ggaacagcag ttgcagacaa atttataaaa gatgctggaa taactcaatc ttatgtagta
      901 ggtggagaga gttcaatttc tgaagctgtt aaaaataaac taccaaatag tacaagacta
      961 ggcggaacag atagaaatga tacaaatgca aaagtaataa aagaatttta taaaaagaca
     1021 gatttaaaaa atgcttatgt aactaaagat ggaatgaata aacaagacca acttatagat
     1081 gcactagcag taggtgtatt aggtgctaaa aatcaatcac cagtagtttt agttggaaag
     1141 aacttatcag cttcacaaaa atctctagtt aactctaaga gttttgataa gataactaaa
     1201 gttggtggaa atggtaatga gacagcattt aatgaaatga aatcactaca agaagtaaaa
     1261 acagttgaag ctaaaacaat ttctgaatta aaatcagcta tagataaagc tactgctaat
     1321 gatgttataa actttaagcc aactagtgag gttaaagaag catttacaat acaaactgat
     1381 aaagcagtaa cagtaaactt aaatggtaca tatacaaaga ctgtgactat caacatgcct
     1441 aatggtgatg taaataacta tgcaaaagtg gatgatgtag ttatagatga tgtaaaagat
     1501 ggaacttttg ttaactatgg aaagataacc aacttaaagg ttaatgataa aaatggagct
     1561 aagatagaga ataattctaa aggtgagatt ggtagtttaa cagtagcatc tggagcaagt
     1621 caagtaaaag tgactaatgg tggaaagata actacagtta caaataattc taaagggact
     1681 actatagata ataaaggtac aataagtagt gttaagggag ataattctcc aacaataagt
     1741 ggaaatagtc caagttctaa ctcttcagga ggaagttcat caagtggagg ttcttcacat
     1801 ggaggaggta gtggaggaag ttcttcatca aaggtaggtc aaataaaatt taatgcaaca
     1861 gataaacaaa taaaaatggc atcaggtgat aagacagtag atccatcaga taacacatat
     1921 gtactaaaat taacaaatgg gacagtaaaa gatggagatg taaaagctaa tgttgaagta
     1981 acaggattac catcaggatt ggattataca gcagtaggaa ataaatcagc aaacacaata
     2041 acaataacag tatcaggaac agcaagccaa gcattacaag ctgatttaga taatgtaagt
     2101 gtgttagtta aagcaggagc agtatctgaa acaactgcta cagattcaat tgcaaatgca
     2161 acatttgaag taaaaaaata tgtggcagca ccagcagtag gtcaattaac ttttgatgca
     2221 acagataaac aaataaaaat gg
//
LOCUS       DAFNNB010000256         2240 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0271, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000256 DAFNNB010000000
VERSION     DAFNNB010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2240
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0271"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            125..577
                     /locus_tag="L3M60_003475"
     CDS             125..577
                     /locus_tag="L3M60_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBN6228094.1"
                     /translation="MNYIVKDTISFLVKTFPKIYSSLYLEDLKKFAPDYNVNKTQLRA
                     LVFIKNYGVISMTDLCSKLNIEKGSLTSMVDDLTDKKYVTRKRDLVDRRKYLIDITEE
                     GDKIATDFMDKLSDGLGEKLSKLTEEDRKKYLEAINTLQYILNKEEFR"
     gene            complement(796..1992)
                     /locus_tag="L3M60_003476"
     CDS             complement(796..1992)
                     /locus_tag="L3M60_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBN6228095.1"
                     /translation="MNYSNRVSAMQASPIRKLVPFAQAAKDKGIKVYHLNIGQPDIKT
                     PKGFFDAVKNFDSEVLEYATSEGIPELLEALQNYYKTYNMNFEKDELLVTNGGSEALL
                     FTMMAVCDPGDNLLVPEPFYTNYNGFGQSVNVEVNAVTTKAENGFHLPSKEEILSKVD
                     DKTKAIILSNPGNPTGAIYTKEELNILAEIAKEKDLWIIADEVYREFVYDGLEYTSCG
                     NLEGVQDRVIIIDSVSKRYSACGARIGSIACKNKGLIAQILKLCQGRLCVPTLEQIGA
                     VELYKTPVSYFKEVNEEYKKRRDVLYNELMKVEGVICKKPTGAFYIVAKLPVENAEDF
                     TIWMLKEFNKDNETVMVCPAEGFYATPGLGRDEIRLAYILNEKDLHRAATLLKEGLEQ
                     YVALQK"
BASE COUNT          799 a          315 c          294 g          832 t
ORIGIN      
        1 gataagatta ctaattaaat ttaaacattc agcaaaaact attgtacttg caatattttt
       61 aatgtaaact gaaataaagt tatttagata atttaataaa ttttaaaaca accgaggtga
      121 ttatatgaac tatatagtaa aagataccat aagtttttta gtaaaaacat ttcctaagat
      181 ttattctagc ctgtatttag aagacctaaa aaaatttgca ccagattata atgtaaataa
      241 aacacaatta agagcattag ttttcataaa aaattatgga gtaataagta tgactgattt
      301 atgctctaag ttaaatatag aaaaaggcag tttaactagt atggttgatg atttaactga
      361 taagaagtat gtaacaagaa aaagagattt agttgataga agaaaatatc taattgatat
      421 tactgaagag ggagataaga tagcaactga ttttatggat aagttaagtg atggtttagg
      481 agaaaaactt tctaaattaa ctgaagaaga cagaaaaaaa tatttagaag caataaatac
      541 tcttcaatat atattgaaca aagaagaatt tagatagaaa aaatacaaaa atttttatat
      601 ttaatagtat taagattaac tttttgttat gaatttagct atataattat aaatctaaat
      661 acaatctaaa aagtatatct taaattttct tgatgtgctt ttttataatc tctataaaaa
      721 atatcccctt ttcaaataat atctgaaaag gggatatttt ttatcattaa atttatataa
      781 attaatttta aaaaattatt tttgtaaagc tacatattgc tctaaacctt cttttaataa
      841 tgtagcagct ctatgaaggt ctttttcatt taggatgtaa gctaatctta tttcatcacg
      901 tcctaaacct ggagtagcat agaatccttc agcagggcaa accataactg tttcattatc
      961 cttgttaaat tcttttaaca tccaaattgt gaagtcttct gcattttcaa ctggtaattt
     1021 agcaactata tagaaagcac cagttggttt tttacatata actccttcaa ccttcattaa
     1081 ttcattgtat aatacatctc ttcttttttt gtactcttca ttaacttctt tgaagtaaga
     1141 aacaggagtt ttatataatt caacagctcc tatttgctct aatgtaggaa cacaaagtct
     1201 tccttggcat aatttaagta tttgagctat aagaccttta tttttacaag cgatagaacc
     1261 aattctagca ccacaagcac tgtatctctt agaaacactg tctattatta taactctatc
     1321 ctgtacaccc tctaagttac cacagcttgt atactcaagc ccatcataaa caaactctct
     1381 ataaacttcg tctgctatta tccataaatc tttttcttta gctatttcag ctaggatatt
     1441 aagttcttct tttgtataaa ttgcaccagt tggatttcct gggttagaaa gaattatagc
     1501 cttagtttta tcatctacct tagacagtat ttcttcttta gatggaaggt ggaaaccgtt
     1561 ttctgctttt gttgtaacag cattaacttc tacgtttaca gattgaccaa atccattgta
     1621 gtttgtgtag aatggctctg gaactaatag attatctcct gggtcacaaa ctgccatcat
     1681 agtgaaaagt aaagcttcac tacctccatt agttactagt aattcatctt tttcaaaatt
     1741 catattgtaa gttttatagt aattttgaag tgcttctaat aattctggaa taccttctga
     1801 tgtagcatat tctaagactt cgctatcaaa gtttttaaca gcatcaaaaa atcccttagg
     1861 agtttttatg tctggttgtc ctatgtttaa gtgatatact ttgattcctt tatcttttgc
     1921 tgcttgtgca aatggaacaa gttttctgat tggagaggct tgcattgcac taactctgtt
     1981 tgaataattc attttaatac cccctattta atttaaatac gtatattgta ttatgtaaat
     2041 tgtatgtttg aatacaagac atctttacaa gataataata acatacttat gggtaaaaat
     2101 catcaaaaaa aattaatata gttttaaaat aaacttttaa caaattatag ttaaataccc
     2161 atgtttatgc cactaaatta tgtatttatg aattaattta tggtaagata atattctgat
     2221 acactgatag taatttttta
//
LOCUS       DAFNNB010000257         2238 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0272, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000257 DAFNNB010000000
VERSION     DAFNNB010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0272"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            94..234
                     /locus_tag="L3M60_003477"
     CDS             94..234
                     /locus_tag="L3M60_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228096.1"
                     /translation="MEKSNTTVTKKEKIEDIGMKLEHLLSEDITFYELLLEILYKEKE
                     EN"
     gene            complement(231..602)
                     /locus_tag="L3M60_003478"
     CDS             complement(231..602)
                     /locus_tag="L3M60_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228097.1"
                     /translation="MESLGDRIANLRKELDINQKELATKVGITEASLSRYENNLREPK
                     SEIIVRLAKALETSTDYLLGVNDNTNISKEDKLIIENLSVSEKTKKLLEKIYSLEKED
                     REAIEKMIDNAYLKRFLKEEN"
     gene            1146..1403
                     /locus_tag="L3M60_003479"
     CDS             1146..1403
                     /locus_tag="L3M60_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228098.1"
                     /translation="MIIIRSQDKTNLMYINQIKIDGSQVYAVFESNLDTVKIGDYENN
                     TRAIQVLDNIQNFIENGTKYDYITFNKVRYNVNKIFQMPVK"
     gene            complement(1513..2193)
                     /locus_tag="L3M60_003480"
     CDS             complement(1513..2193)
                     /locus_tag="L3M60_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBN6228099.1"
                     /translation="MIWTFYSFICVILPCLIYQIVVIKRKNLKVEKNVVIHLVWVYIF
                     LLYIYLALSKAGIGSIWDIGRYSDLFRVDEINLIPFDSVGILTYILNVIMFMPLGFLL
                     PLIWKNFRNIINVLLTGLGFSLAIEFCQLFNLRATDIDDLMMNTLGAVLGYFIWVGVN
                     NLFNLIKKKDIFPSIYKNIIQDEIAITIDDDISKKITSLYKNEAIIYLILAVLGEFLL
                     FNWSIIPY"
BASE COUNT          903 a          260 c          257 g          818 t
ORIGIN      
        1 aaaatcaaaa tacaattaca taaactttat taaaaatttg ttgggaattt ctgactaaca
       61 ttaagaacgt aagtttgttg ggggatggta tatgtggaaa agtcaaacac tactgttaca
      121 aaaaaagaaa aaattgaaga tataggaatg aaattagaac atttattgag tgaagatatt
      181 actttttatg aattattact tgagattttg tataaagaaa aagaagagaa ttaattctct
      241 tctttaagga atctttttaa ataggcatta tcaatcattt tttctatagc ttctctatct
      301 tctttttcaa gagaatatat tttttctaaa agtttttttg ttttttcact cactgataag
      361 ttctctatga ttaatttatc ttctttgctt atgtttgtgt tatcatttac gcctaataaa
      421 taatctgttg atgtttctaa tgcttttgct aatctcacta ttatttcact tttaggttct
      481 cttaaattat tttcataacg tgacaagcta gcctcagtta taccgacttt agtagcaagt
      541 tctttttgat ttatatctaa ttcttttcgt aaatttgcta ttctatctcc taaactttcc
      601 atatgtacat cactccttac cgaaattata agttattttt ataaaaagta aaataaaatt
      661 accaaaaata taaaaaaaaa gaattaaaat aagaaaatat attgaaaatt accatttgta
      721 tatgtataat aagaattata caaatggtaa tttgatgaga ggtggtattt tgaaataaga
      781 aaccgaatta attagtaaac atatagaaag ttttgaatac tgttacttga gatataaaca
      841 atatgcataa aaatttttga aagtaaaatt accaaatgta taaaataaat cagtagcata
      901 gatagtttga tgctttaata gattatgtaa tttagttata atagccaaaa atgcaattta
      961 ccaataaaac aaggtattat aaaaaaagta ttctttaagg gaacttttta ttagatatgc
     1021 aattgataca aataatcgta ttaaaaaata aaatactaag tttaaaaatt taaataatct
     1081 agaaatcaat tataaaatat tatttcttag aaactaaaat taattaagta aatggaggaa
     1141 ttgacatgat aataattaga agtcaagata aaactaattt aatgtacata aaccaaatta
     1201 aaatagatgg gagtcaagtt tatgcagtat ttgaaagtaa ccttgatact gtaaaaatag
     1261 gagattatga aaataataca agagctatac aagtattgga taatatacaa aacttcattg
     1321 aaaatggaac aaaatatgat tatataacat tcaataaagt cagatacaat gttaataaaa
     1381 tatttcagat gccagttaaa taatgagtgt gcattttagt ttaataacta aagcccgctt
     1441 tcttttgaag tatgaaagag ggctttacac atttgtaaat attgaagtac acttgttttg
     1501 tcagtaaatt atttaataag gtataatgga ccaattaaac aataaaaatt ctcctaaaac
     1561 agcaagtatt aaatatataa tagcttcatt tttataaaga gaggtaattt ttttgcttat
     1621 atcatcatca attgtaatag caatttcatc ttgaataata tttttatata tagatggaaa
     1681 aatatctttt ttctttataa gattaaataa attatttaca cctacccata taaaatatcc
     1741 taaaacagca cctaatgtgt tcatcattaa atcatcaata tctgtagctc ttaagttaaa
     1801 caattgacaa aattcaattg ctaatgaaaa acctaatcca gttaataaaa cattgataat
     1861 atttctaaaa tttttccaaa ttaaaggtag taaaaatcct aaaggcataa acataattac
     1921 atttaaaata tatgttaaaa tgccaactga gtcaaaaggt attagattta tttcatcaac
     1981 tctaaaaagg tcactatacc ttcctatatc ccatatgcta ccaataccag ctttactaag
     2041 tgctaggtat atatataata aaaaaatata tacccatact aaatgtatca ctacattttt
     2101 ttcaactttc aaattttttc tttttataac aactatttgg tatattaagc atggtaatat
     2161 aacacaaata aatgaataaa atgtccatat cattttattt tgtccccttt caaatttatg
     2221 tttttagttt tataagta
//
LOCUS       DAFNNB010000258         2178 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0275, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000258 DAFNNB010000000
VERSION     DAFNNB010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0275"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            226..1752
                     /gene="istA"
                     /locus_tag="L3M60_003481"
     CDS             226..1752
                     /gene="istA"
                     /locus_tag="L3M60_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HBN6228100.1"
                     /translation="MALLSVIRRWHFRDDLSIREICRRTGLSRNTIRKYLRGGDVEPR
                     FKVPERPSKLDPFADRFSVWLKTESKKSRKQKRTMKQLHSDLVSLGYDGSYCRVAAFA
                     REWKADRQRELQTTGRGTFVPLAFEPGEAFQFDWSEDWAVIGNERTKLQVAHTKLSYS
                     RAFIVRAYLLQTHEMLFDAHNHAFRVFGGIPGRGIYDNMRTAIDKVGRGKERDVNIRF
                     MAMASHYLFEPEFCNPAAGWEKGQVEKNVQDARHRFFQPTPRFPSLEALNDWLEIQCK
                     EFWAKTLHGQMRGTIADIWAEEAPALMPVSRPFDGFVEYTKRVTPTCLVHLERNRYSV
                     PASFANRPVSLRVYPDRIVVAAEGQIVCAHHRVIDRSHDRPGQTIYDWRHYLAVVQRK
                     PGALRNGAPFAELPDAFKNLQQYLLKKPGGDREMVDILALVLQHDEQAVLKAVEMALK
                     SGVPTKTHVLNLLHRLVDSKSFTPPTLDAPQALTLTNEPKANVERYDTLRKTEVRHAS
                     "
     gene            1742..>2178
                     /locus_tag="L3M60_003482"
     CDS             1742..>2178
                     /locus_tag="L3M60_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6228101.1"
                     /translation="MRHNPASGAIVIMLRQLKMHGMAQAVGELTEQGAPAFEAAIPIL
                     SQLLKAETAEREVRSTAYQLKTARFPAYRDLNGFDFSSSEINEALVQQLHCCNFLDDA
                     NNIVLVGGPGTGKTHVATAIGVQAIEQHHKRVRFFSTVELVNAL"
BASE COUNT          478 a          626 c          636 g          438 t
ORIGIN      
        1 aagatgacat gggcgtagtg gaacagaccc gctgccatgc acgatccgac aacgaggatc
       61 cgcttaacag cgacgggtgt tgcctccaac gtgtcgggtg ttctgaattt ataggtgttg
      121 atttgcattg agagctgacc cgggattgcg ggaaaatatc actgagaatt gacccatgtt
      181 tcaatcatcc cctgtggttt cagcgggggc actggagtga tcgacatggc gttactgagc
      241 gtaatccgac gctggcattt tcgggatgat ctatcgatcc gggagatatg ccgcaggacg
      301 ggcctatcgc ggaatactat ccgcaaatac ctgcgtggcg gcgacgtgga gccgagattc
      361 aaggtaccgg agcgaccgag caagcttgat ccgtttgcag atcgtttctc ggtctggctg
      421 aagacggaat ccaaaaagag ccgcaagcag aaacggacga tgaagcagtt gcattctgat
      481 cttgtgagcc ttggctatga cggctcctac tgccgcgttg cagcctttgc tcgcgaatgg
      541 aaagcggacc ggcagagaga actccagaca acaggacgcg gcacgtttgt ccccctggca
      601 tttgagccgg gtgaggcgtt ccagttcgac tggtctgagg attgggcggt gattggcaat
      661 gagcgcacga agctgcaggt ggcgcatacg aagctgagct acagccgcgc ctttattgtc
      721 cgggcctatc tgctgcaaac gcacgagatg ctattcgacg cgcacaatca tgcgttccgc
      781 gtcttcggcg gaattcctgg ccgcggcatc tacgacaaca tgcgcacagc gatcgacaag
      841 gttggccgtg gaaaagagcg cgacgtcaat atccgtttca tggcaatggc cagccattac
      901 ctgttcgaac ccgagttctg taatcctgca gccggctggg agaagggaca ggtggagaag
      961 aacgttcagg acgcgcgtca tcgcttcttt caaccgacgc cgcgctttcc gtcactggaa
     1021 gccctaaatg actggcttga gattcaatgc aaggagttct gggcaaagac tctacatggc
     1081 cagatgcgcg gcaccattgc cgacatctgg gcagaggaag cgccggctct catgcccgtt
     1141 tccaggccgt tcgatgggtt cgtcgagtac acgaaacggg tcacaccaac ctgcctcgtg
     1201 catctggagc gcaaccgcta cagcgtcccc gcttctttcg ccaatcggcc agtgagcctg
     1261 cgggtctatc ccgatcgtat tgttgttgca gcagaaggtc agatcgtctg cgcgcatcat
     1321 cgcgtcatcg atcgttccca tgatcggccg ggacagacca tctacgactg gcggcactat
     1381 ctggctgtcg tgcagcgcaa gccgggcgct ctgcgcaatg gtgctccgtt cgccgagcta
     1441 ccggacgcat tcaagaacct gcagcaatat ctgctcaaga agcccggagg cgaccgcgag
     1501 atggtcgata ttctggcgct tgtccttcag cacgacgagc aagccgtgct gaaggctgtg
     1561 gagatggcgc tgaagtccgg ggttcccacc aagacgcatg tgctgaacct gctgcatcgc
     1621 ctcgtcgaca gcaaatcctt cacgcctcca accctcgacg cccctcaggc actgacgctt
     1681 accaacgagc ccaaggccaa tgtggaacgg tacgacacct tgagaaagac ggaggtccgc
     1741 catgcgtcat aatccggcaa gcggcgcgat cgttatcatg ctgcggcaac tgaagatgca
     1801 cggcatggcc caagccgtcg gtgaactgac cgaacaagga gcgccggcgt tcgaagctgc
     1861 cattcccatc ctgtcgcaac tcctcaaggc ggagacggcc gaacgagagg tgcggtctac
     1921 ggcctatcag ctcaagacgg cacgctttcc tgcctaccgc gatctgaacg gatttgactt
     1981 tagcagcagc gagatcaacg aggcgctcgt acaacagctt cactgctgta acttcctcga
     2041 cgacgccaac aacattgtcc tggtcggcgg ccccggtacg ggcaaaacgc atgttgccac
     2101 agcgatcggt gtccaggcca tcgagcagca tcacaagcgc gttcggttct tctcaactgt
     2161 ggagctggtc aacgcgct
//
LOCUS       DAFNNB010000259         2177 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0276, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000259 DAFNNB010000000
VERSION     DAFNNB010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2177
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0276"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            373..771
                     /locus_tag="L3M60_003483"
     CDS             373..771
                     /locus_tag="L3M60_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6228102.1"
                     /translation="MDTLGKRIAYLRNSKKLTQRKLMDILKFENLGKYETGDRKPNCD
                     IVMSIADYFNVTTDWLLYGKEKVNSNNSVKEDKEDYLHINNDEMTILNLYRQLNERDK
                     IKIEGILELKISEYKDLKKHSSNNNEDKMV"
     gene            complement(988..1197)
                     /locus_tag="L3M60_003484"
     CDS             complement(988..1197)
                     /locus_tag="L3M60_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228103.1"
                     /translation="MCKLDSDGVIECCKAIDDFITALSNIKSLNMERLNILTKYSSTC
                     SILLKEGNYEGCTIVYRKMLEELKT"
     gene            complement(1229..1438)
                     /locus_tag="L3M60_003485"
     CDS             complement(1229..1438)
                     /locus_tag="L3M60_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228104.1"
                     /translation="MDARRKWIPFLGVQVKQRLIELNMTQRELAKKIGVNENYLSAIL
                     NGRRTGKKYKSSIYQLLNIEYSEDD"
     gene            1734..2153
                     /locus_tag="L3M60_003486"
     CDS             1734..2153
                     /locus_tag="L3M60_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228105.1"
                     /translation="MDTLGERIVYLRKAKNLKQYELEEMLGCDNLSKFERNIRKPNYE
                     ILKSISEIFNVSVDWLLNGDNLSHKSDLICDSSSNYPLNSINSNEIKLLNNFRKLNDY
                     DKAKIEGMIELKLHEYEKDKNLKKTEYNKTKDEKIDK"
BASE COUNT          848 a          269 c          237 g          823 t
ORIGIN      
        1 attttatcct aaagaataaa tgcataaaaa tttttgtaat ggattaatat aaattacaat
       61 ataatactat ttttctatta atctttttta ctattaaagg aatcaaaagg tatcttagct
      121 tatctaaata aatttataca atctattcat acacaccttt acatttttat aacaattaaa
      181 cttattgtta tatacaaatc aaaataaacc atatattttc aactataata tgttaaaatc
      241 atattatgag catattaatt taagcaaaat gtcatttaga ctttaattta gtagttttat
      301 attacaattt tttatatttt taattttaaa tgtcatttag acattattta taaaaagaaa
      361 ggagtgtatt ttgtggatac tttaggaaaa agaattgctt atttaagaaa ctccaaaaaa
      421 cttactcagc gcaaacttat ggatatttta aaatttgaaa acttaggcaa gtatgaaaca
      481 ggagatagaa aacctaattg cgatatagta atgtcaatag ctgattattt taatgttacg
      541 actgactggc ttctctatgg taaagaaaaa gtaaattcca ataatagtgt aaaagaagat
      601 aaagaagatt acttacatat taataatgat gaaatgacta tattaaatct atatagacaa
      661 ttaaacgaac gtgataaaat aaaaatagaa ggaatacttg aacttaagat atctgaatac
      721 aaagacttaa aaaagcattc ctctaataat aatgaagata aaatggttta aacatacata
      781 atttgatacg attttacatc atctattaac tttctttttt ggtagtatat tactaaaata
      841 tggatattgg attatttaaa aacaagtata gtctaatttc aagcacattt ataatattta
      901 gattatctta tccatatttt agtttacgta ctttgaaaaa tataatacat ttctatgtat
      961 aatttatatt cctaagaaat gtattgctca tgtttttaat tcttccaaca tctttctata
     1021 tacaattgta catccttcat aattcccctc tttaagaagg attgaacatg tactagaata
     1081 tttagtcaaa atatttaacc tttccatatt taagcttttt atattactaa gtgctgtaat
     1141 aaaatcatca attgctttac aacactctat aactccatct gaatccaatt tacacacaag
     1201 aatattcacc tactttatat actatttatt aatcatcttc tgaatattct atattaagca
     1261 attgataaat tgatgattta tattttttac ctgttcttct cccatttaaa atagctgaca
     1321 aatagttttc attaacacct attttcttcg ctaattctct ttgagtcata tttaattcaa
     1381 taagcctttg cttgacttgt actcccaaaa aaggtatcca ttttcttcga gcatccattt
     1441 atcaacttcc tttctaagta atttatattt acttttatat tttagtttac gcaccccctc
     1501 tatttagtat atatttttaa tatgtacaca tattaagtat ataaatttat ttaatactaa
     1561 ataagatttt ttgattactt tgtgctaaaa ttaggatgta aattcgtatc attaagttaa
     1621 ttataaagtc attttgacct taagtcaatg atttttatgt cattttgacc tttattttaa
     1681 aactatttat gtcttaaaaa gtctttttga cattaattga atggagagtg tttatggata
     1741 ctttaggaga aagaattgtt tatttgagaa aagcaaaaaa ccttaaacaa tatgaactag
     1801 aagaaatgtt gggctgtgac aacttgagta aatttgagag aaacattaga aaaccaaatt
     1861 atgaaatatt aaaatctata tctgagatat ttaatgtttc agtagactgg ctattaaatg
     1921 gagataatct atcacataaa tcagatttaa tttgcgattc ctcctcaaac tatccactta
     1981 atagcataaa ctctaatgaa ataaaacttt taaacaactt tagaaaatta aatgattatg
     2041 acaaagccaa aatagaaggt atgattgaat taaaacttca tgaatatgaa aaagataaga
     2101 atttaaaaaa aactgagtat aataaaacta aagatgagaa aatagataaa taatatttat
     2161 gttgcttttg atacgat
//
LOCUS       DAFNNB010000260         2133 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0277, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000260 DAFNNB010000000
VERSION     DAFNNB010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0277"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(67..444)
                     /locus_tag="L3M60_003487"
                     /pseudo
     CDS             complement(67..444)
                     /locus_tag="L3M60_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
     gene            complement(375..596)
                     /locus_tag="L3M60_003488"
     CDS             complement(375..596)
                     /locus_tag="L3M60_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228106.1"
                     /translation="MNKYNKTLKKNKIFQGISRKATFFDNSPMKNSFGVMKRECIIKK
                     YKQLFESFFVIIKRIMRGLRNLDMKVLLS"
     gene            958..1308
                     /locus_tag="L3M60_003489"
     CDS             958..1308
                     /locus_tag="L3M60_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HBN6228107.1"
                     /translation="MIQKKFFMGFCGFIGFLSLRYFSSGNIADLTYIGFFAFFSNFII
                     AKINGDKADERYVQDEKSAMAFTGQFAIIELFILWCITIVSRNVELMCVLLSIAYAIT
                     LNVYAIKLYILEEK"
     gene            1310..1510
                     /locus_tag="L3M60_003490"
     CDS             1310..1510
                     /locus_tag="L3M60_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228108.1"
                     /translation="MPKFICNLKKYRQLKELTQEQLAEKVGVRRETIMRLEAGKYNPS
                     LKLAIDISKIVDTPIDELFIFD"
     regulatory      1793..1882
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1936..2112
                     /locus_tag="L3M60_003491"
     CDS             1936..2112
                     /locus_tag="L3M60_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228109.1"
                     /translation="MFRDKMDKCTHMLTAYIGSSYDYCDFIDTQLDDFIIEYGEKVVE
                     SCLHQVMVLVSKYN"
BASE COUNT          766 a          280 c          271 g          816 t
ORIGIN      
        1 aaaaattgtc agatataaaa agctagaaaa aggaaggtaa caacctacta gttgctacct
       61 tttaatctat ctatttataa aatctaatac tttgtaaagt gtatcaaatc tatcactacc
      121 ctttatcatg gtatagtttt ctttagttat agaacctatc ttctcacatg ctccaccaac
      181 aacaacataa agattttcag ttcttcctgg tacataatct tttatattac atattaaaat
      241 tttatttgaa ccataacccc aaccaagcac agttgcaggg attttatcaa cttcaccatc
      301 ataaactatt gtatgtttgt acatctgttt aactccctca ttatttatat ttttatttaa
      361 tacaccttct acaattaact tagcaatacc ttcatatcta agtttcttag ccccctcata
      421 atcctcttta ttattacaaa aaaactttca aataattgtt tatatttttt tataatacat
      481 tctcgcttca ttactccaaa tgaatttttc ataggagagt tatcaaagaa tgtagcctta
      541 cgagatatac cttgaaatat tttatttttc ttaagtgttt tgttatactt attcatttta
      601 tatggccaac cttggtttga gttaaaagtc cttctataag gacaatcctt tgtaatattg
      661 atagctttat ttaaaacatc actattgatt taaagttagc catatatcag acactgaatg
      721 acataacttc tccttcttct ctatttaaat aaaatttagc tacttttaat ttaaatttaa
      781 aatcgtattt agtcatacat aaacacctca atcattagat ttttggtcta gtaattatgg
      841 tacattacaa atattgtata acactgcttt tatattgaca aaaaaataaa aaagagatat
      901 atttatatca taaagataca aatatatctc tttttaaaat actaaggagg ataagaaatg
      961 attcagaaaa aattttttat gggtttttgt ggtttcattg gatttttaag tcttagatat
     1021 ttttcatctg gtaatattgc tgaccttaca tatattggct tttttgcttt tttctcaaac
     1081 tttattatag caaaaataaa cggtgataaa gcagatgaaa ggtatgtaca agatgaaaaa
     1141 tcagctatgg catttacagg acagtttgct attatagaat tatttatttt atggtgcatt
     1201 actatagtgt ctagaaatgt agaattaatg tgtgttttac tatcaattgc atatgcaatt
     1261 actttaaatg tttatgctat aaaactttat attttagagg aaaaataaca tgcccaaatt
     1321 catttgtaat ctaaaaaaat acagacaatt aaaagaatta actcaagaac aattagcaga
     1381 aaaagtagga gttagaagag aaaccattat gcgtttggaa gcaggtaagt ataatccttc
     1441 tttaaaatta gcgatagata tatcaaaaat agtagatact cctattgatg aattgtttat
     1501 ttttgattag tttttgcatt attaaaaaca cgttttaaat tcaaacttaa attcaatttt
     1561 tagtcactcc tcccagaggg gctttttatt tttttaataa tttattagtt aagtaaaata
     1621 ttaaactggc aactgaattt actagtatac cttgtaaaaa attatcaatg tgtgttcacc
     1681 tctcttttta atattgaaaa aataatttaa cattaatata taaatttatt aatttttcag
     1741 ctagatatgt tgaataataa ctaattctat tgtattatta gtttgtagaa taaaactaaa
     1801 tcggcaaaac tagagaaatt tagtgacgca aagctatagg gactaacgct tataaaataa
     1861 gttatgtcag ccagttgcca aaaagatatt ttctttttgt ttttcatgaa agttttttag
     1921 ggagataaat acattatgtt tagagataaa atggataaat gtacacatat gttaactgct
     1981 tatattggta gttcatatga ttattgtgat tttatagata cacagttaga tgattttata
     2041 atagagtatg gagaaaaagt tgtagaatct tgcttgcatc aagtgatggt attggtaagt
     2101 aagtataatt agacagattt ttttctagaa aac
//
LOCUS       DAFNNB010000261         2131 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0278, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000261 DAFNNB010000000
VERSION     DAFNNB010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0278"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(168..704)
                     /locus_tag="L3M60_003492"
     CDS             complement(168..704)
                     /locus_tag="L3M60_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBN6228110.1"
                     /translation="MEYKSPVYGIIAVPIEKIESNDYNPNAVAPPEMQLLYKSIKEDG
                     YTMPIVCYYNEEKDKYIIVDGFHRYRIMLENEDIYDRENGLLPVSVIDKSLDERMAST
                     IRHNRARGSHNVDLMSKIITELCEIGKSDEWISKHLGMEIDEILRLKQITGLAYLFKD
                     KEFGNAWIPFGSDVEEEN"
     gene            complement(722..2035)
                     /locus_tag="L3M60_003493"
     CDS             complement(722..2035)
                     /locus_tag="L3M60_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HBN6228111.1"
                     /translation="MDKRYLESDVFEAFQERLSYLFNEFDYIYVSFSGGKDSGLLLNL
                     VLDYKRKNNISSKIGVFHQDFEAQYSLTTEYVEKVFEENIEDIEPYWVCLPMATRTAV
                     GSYEMYWYPWDDEKEELWVREMPVKEYIINQKNNPFTYYKYKMRQESLAKQFGRWIRD
                     IHEGAKVVCLLGTRASESLQRYCSIVNKQYGYKGKCWITKEFKNVWTASPIYDWEIED
                     IWLAHYKFDYDYNKIYDLFYKAGIAPRKMRVASPFNDSAKESLNLYRILDPDVWVKLV
                     ARVQGVNFTSIYGKTKAMGYRSVRLPYGHTWKSFTYFLLDTLPENIRNNYIKKFSTSI
                     EFWQNTGGGLSEIVIRELLRKGYKIELNGVSNHTKDKKSRVIFLDDIPDDTDDIKFTK
                     DIPSWKRMCYCILKNDHMCRFMGFGPTKEQRIRTEYIKKKYEKIH"
BASE COUNT          672 a          406 c          213 g          840 t
ORIGIN      
        1 tatttaccta tataatttta tttatttttg gagtataatt ataaaaaaaa gaataggaca
       61 ttttcctact cttttctctg tacttatata atattaaaac tctttatttt ttaatatata
      121 ttttaggttt tcttttttaa tatatctttt tcttttaata aatatcttta gttttcttct
      181 tctacatcag agccaaaagg aatccatgca ttaccaaact ctttatcttt aaataaataa
      241 gctaatccag ttatttgctt caatctaaga atttcatcaa tttccatacc caggtgcttt
      301 gatatccatt catcagattt tccaatttca caaagttcag ttataatttt actcattaaa
      361 tccacattat gagagcctct tgcacgattg tgtcgaatag tacttgccat tctttcatca
      421 agagatttat caattactga aacaggaaga agtccgtttt ctctatcata tatatcctca
      481 ttttcaagca ttattctata gcgatgaaaa ccatctacaa taatatactt atctttttcc
      541 tcattataat aacagacaat tggcattgta tacccatctt cttttatact tttgtacaac
      601 aactgcattt ctggtggggc tactgcattt ggattatagt cgttagattc tatcttttcg
      661 attggcactg cgatgattcc atatacagga cttttatatt ccatagcatc ttaactccta
      721 tctaatgaat tttttcatat ttcttcttaa tatattctgt tctaattctc tgttcttttg
      781 taggaccaaa gcccataaac ctacacatat ggtcattttt taaaatacaa taacacattc
      841 ttttccagct tggtatatcc ttagtaaatt tgatatcatc tgtatcatca ggtatatcat
      901 ccaaaaatat aactctactc tttttatctt ttgtatgatt tgaaactcca tttaattcta
      961 ttttataacc ttttctcaac aattcacgga taactatttc tgacaaacca ccacctgtat
     1021 tctgccaaaa ctcaatagat gttgaaaatt ttttaatata gttatttctt atattttcag
     1081 gtaaggtatc aagcaaaaaa tatgtaaaac ttttccacgt atgtccatat ggtaacctaa
     1141 cactacgata tcccatagcc tttgtttttc cataaataga ggtaaaatta actccttgta
     1201 ctctagcaac caacttcacc catacatctg ggtccaatat tctgtataaa tttaaactct
     1261 ctttagctga atcattaaaa ggtgatgcca ctctcatttt tcttggagct attccagctt
     1321 tatagaataa gtcataaatt ttattatagt cataatcaaa cttatagtgg gccaaccata
     1381 tatcttctat ttcccaatca tatataggag atgctgtcca gacattttta aattccttag
     1441 ttatccaaca ctttccttta tatccatact gtttattcac aatagaacaa taacgttgta
     1501 gagattctga tgctcttgtt cctaataagc aaacaacttt tgcaccttca tgtatatctc
     1561 taatccatct accaaattgt tttgccaaac tttcttgcct cattttatat ttataataag
     1621 taaaaggatt atttttttga tttattatgt attctttgac tggcatttct cttacccata
     1681 attcctcttt ctcatcatcc catggatacc aatacatttc ataacttccc acagcagttc
     1741 ttgtagccat tggtaaacac acccaatatg gttcaatatc ttcaatgttc tcttcaaata
     1801 ctttttcaac atattcagta gtcaaactgt attgagcttc aaaatcttga tgaaatactc
     1861 ctattttgga acttatatta ttctttctct tataatccaa aactaaattt agcagtaaac
     1921 cactatcttt tcctccacta aaagatacat agatataatc aaattcattg aaaagataag
     1981 aaagtctttc ttgaaaagct tcaaatacat cggattctaa atatctctta tccatttgaa
     2041 ttatcccctt tcaattagaa gtttataaaa tcttacaatg caatattatt atgctttttt
     2101 agcagtaatc agcataaatt atacattttt a
//
LOCUS       DAFNNB010000262         2124 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0279, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000262 DAFNNB010000000
VERSION     DAFNNB010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0279"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            48..>2124
                     /locus_tag="L3M60_003494"
     rRNA            48..>2124
                     /locus_tag="L3M60_003494"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          632 a          424 c          607 g          461 t
ORIGIN      
        1 aaaattatcg aagttgatag cttctaatct atcaaacctt tttaactggt caagttatta
       61 agggtgcagg gcggatgcct tggcactagg agccgatgaa ggacgtgata agctgcgata
      121 agcttcgggg agttgcacgt aaactttgat ccgaagattt ccgaatgagg aaactcactt
      181 agagtaatgt ctaagtatca ttaagtgaat acatagctta atgaggggaa ctcagggaac
      241 tgaaacatct aagtacctga aggaagagaa agaaattcga ttccgtaagt agcggcgagc
      301 gaacgcggat tagcccaaac caataaagtt ttctttattg gggttgcgga catatcataa
      361 cgaagaggta tcgtaattga agaggtttgg aaagacccac cacagaaggt aatagtcctg
      421 tatgttaaac gagaagactt cagatatgat ccagagtacc acgggacacg tgaaaccctg
      481 tgggaagcag gagggaccac cctccaaggc taaatactac ctagtgaccg atagcgtata
      541 gtaccgtgag ggaaaggtga aaagaacccc gggaggggag tgaaatagaa cctgaaaccc
      601 tgcacttaca agctgtggaa gcacatttct tgtgtgaccg cgtacttttt gtagaacggg
      661 ccaacgagtt acgttaagta gcaaggttaa gcacttaagg tgtggagccg tagcgaaagc
      721 gagttttaac tgagcgttca gttacttgac gtagacccga aaccgggcga cctacccatg
      781 agcaggatga agcgaaagta aaatttcgtg gaggtccgaa cccacgagcg ttgaaaagct
      841 cggggatgac ttgtgggtag cggtgaaatt ccaatcgagc ccggagatag ctggttctcc
      901 ccgaaatagc tttagggcta gcctcaaggt gagagatacg gaggtagagc actgaatgtc
      961 ctagggggta ttgcacctac cgaagactat caaactccga atgccgtcat cttatacttg
     1021 ggagtcagac tgtgggtgat aagattcata gtcgaaaggg caacagccca gatcgtcagc
     1081 taaggtccct aaatgtaagt taagtggtaa aggatgtggg attgcacaga caaccaggat
     1141 gttggcttag aagcagccac tcattcaaag agtgcgtaat agctcactgg tcgagtgatc
     1201 ctgcgccgaa gatttccggg gctaaaactt actaccgaag ctacggcatc agtaatgatg
     1261 ggtaggggag cttcccatac gggttgaagc atgaccgtaa ggacatgtgg acagtatggg
     1321 agtgagaatg ttggcatgag tagcgagatg tgggtgagaa tcccacaggc cgtaaaccca
     1381 aggtttccag gggaaggttc gtccgccctg ggttagtcgg gacctaagct gaggccgaaa
     1441 ggcgtaggtg atggacaaca ggttgatatt cctgtactac cgataaccgt ttgagagaag
     1501 ggatgacaca gtaggataag ctaagcacac tgttggttat gtgtgcccaa gcattgaggc
     1561 agtcaaagta ggcaaatccg ctttgataat gctgggatgt gatggggagc gaaatttagt
     1621 agcgaagtag ctgatttcac actgtcaaga aaagtctcta tcgaggttaa aggtacccgt
     1681 accgcaaacc gacacaggtg ggtgaggaga gtatcctaag gccagcgaga gaactgttgt
     1741 taaggaactc ggcaaaatga ccccgtaact tagggataag gggtgccacc atcaggtggc
     1801 cgcagagaat aggcccaagc gactgtttac caaaaacata ggtttctgct aagtcgcaag
     1861 acgatgtata ggagctgacg cctgcccggt gctggaaggt taaggggatc tgttagagca
     1921 atcgaagcag tgaacttaag ccccagtaaa cggcggccgt aactataacg gtcctaaggt
     1981 agcgaaattc cttgtcgggt aagttccgac ccgcacgaaa ggcgtaacga tttgggcact
     2041 gtctcaacaa cagactcggt gaaattgtaa ttccggtgaa gatgccggat acctgcgaca
     2101 ggacggaaag accccatgga gctt
//
LOCUS       DAFNNB010000263         2108 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0282, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000263 DAFNNB010000000
VERSION     DAFNNB010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0282"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..830
                     /locus_tag="L3M60_003495"
     CDS             <1..830
                     /locus_tag="L3M60_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HBN6228112.1"
                     /translation="KENTVGNNITQIDISTDNNLVLYLAYLWLFVTIVIAVYTFIKYT
                     RFKNLVVDVSYSIEDNDVNCIYKNLLKELNIKKKIELRGSDELISPAGMGLFKSYIFL
                     PDYPYSRDELSWILKHELMHFKNKDILIKFLVLSVKIIYWFNPLVYIMSNKVNLDCEL
                     CCDESVLTDCSLKDKKEYALALIKSIKLSRNYNSGILTTEFNKTSLEKRLDSIVKKKG
                     KSGILVLLILFTLFSVTYFNFESISFDNSNSLRNIGILEKSGADLKEENDISMRIVN"
     gene            896..1483
                     /locus_tag="L3M60_003496"
     CDS             896..1483
                     /locus_tag="L3M60_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBN6228113.1"
                     /translation="MKNLKKILIVTGCLTLISTSLVFANTTTDVDTSKDDDSVISSCT
                     ITGYEFTYENAPQSVKFDYEKKCEALNIKPSKDDIIFVNKDSIDVNDIENYVEPKAYI
                     IRYKKGYVFEVTGPKSYTLDVSDTVVGYNRVESGNPVHLVQLLCRTLDGSSIKDDSEF
                     GPATYNAVKSLQGRLGLTRDGIVGRSTWDAAGRRL"
     gene            complement(1699..1988)
                     /locus_tag="L3M60_003497"
                     /pseudo
     CDS             complement(1699..1988)
                     /locus_tag="L3M60_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816433.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          773 a          203 c          337 g          795 t
ORIGIN      
        1 ccaaagaaaa tacagtagga aataatatta cacaaattga tatttctaca gataataatc
       61 ttgtgctgta tttagcttat ttatggttat ttgtaactat agtcattgca gtttatacat
      121 ttataaagta tacaagattt aaaaatcttg ttgttgatgt atcttatagt attgaagata
      181 atgatgtaaa ctgtatctac aaaaacttgt tgaaagaatt aaatataaaa aagaaaattg
      241 agttaagagg ttctgatgaa ttgataagtc cagcaggcat gggattattt aaatcttaca
      301 tattcttacc agactatcct tatagtagag atgaactaag ttggattttg aagcatgaat
      361 taatgcattt taaaaataaa gatattttaa taaagttttt ggttttgtct gttaaaataa
      421 tatattggtt taatccactt gtttatataa tgagcaataa agtaaatcta gattgtgaat
      481 tatgttgtga tgaaagtgtt ttgactgatt gttctttaaa agataagaaa gaatatgcat
      541 tagcacttat aaagtcaatt aaacttagta ggaattataa tagtggtatt ttaacaacag
      601 agtttaataa gacaagttta gaaaagagac ttgacagtat tgttaagaaa aaaggtaaaa
      661 gtggaatatt ggtgttattg attttgttta cattattttc agttacatat tttaattttg
      721 aaagtattag ttttgataat tcaaactctt taaggaatat aggtatttta gaaaaatcag
      781 gagccgatct taaggaagaa aatgatatta gcatgagaat tgtaaattaa tagattaata
      841 tatgtaatga aaaggtatat tgtcatatta ttaataaatt aggaggaatt atgaaatgaa
      901 aaatttaaaa aaaattttaa tagttacagg ctgtctaact ttaatatcta ctagtttggt
      961 atttgcaaat actactactg atgtagatac atcaaaggat gatgattcag taataagttc
     1021 atgtactata actggttatg aatttactta tgaaaacgca ccacaatctg taaaatttga
     1081 ttatgaaaaa aaatgtgaag cgctaaatat aaaaccatct aaagatgata taatttttgt
     1141 taataaagat tctatagatg taaatgatat agaaaactat gttgagccaa aagcatatat
     1201 tattagatat aaaaaaggtt atgtttttga agttactgga cctaagagtt atacactaga
     1261 tgtaagtgat actgtagtag gatataatcg tgttgaatca ggtaatcctg tgcatttagt
     1321 ccaactttta tgtagaactt tagatggttc aagtattaag gatgatagtg aatttggacc
     1381 tgctacatat aatgcagtta agtcacttca aggaagactt ggattaacaa gagatggaat
     1441 tgttggaaga agtacttggg atgcagctgg aagacgtctt taaaaaagag agctattgca
     1501 cagataattt taaattgtta aatctgtaaa catttatgtg tattctttaa ataaaaattt
     1561 gttttttaag tgataattaa atttttactg atagggctat ggctttaaaa atcatagcct
     1621 tatttcgatg ttttttcgtt tttgtaaaat tattgccatt ttaagaaaat ttattgattt
     1681 ttttgaaata tttctttatt aatttagagt taaatttctt tctttaaaat aaatacttaa
     1741 atgagctaaa acttgagtcc aatttctaat tttatgagac cgtttttcaa aaatatccat
     1801 agttgctaag tatagtgatt tgtgcaaaga atcatctgaa ggaaaaatag acttcgattt
     1861 agtaactttt tttaataatc tattgtaact ttccattgag tttgtagtat atatcaatgt
     1921 tcttatccat gaggatattt aaaatatgtt gcgaactcat tccaattgct tcgccaattt
     1981 cttaatgcta cctcttctgt aattgcatca tatactttct ttaatgagtt tttttacatt
     2041 tcgtttttgt taaacataaa aagtgagcgc cttcctttgt atagtgatat ttgatatttt
     2101 aattatta
//
LOCUS       DAFNNB010000264         2045 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0285, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000264 DAFNNB010000000
VERSION     DAFNNB010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0285"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            180..1016
                     /locus_tag="L3M60_003498"
                     /pseudo
     CDS             180..1016
                     /locus_tag="L3M60_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888014.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
     gene            complement(1108..1575)
                     /locus_tag="L3M60_003499"
     CDS             complement(1108..1575)
                     /locus_tag="L3M60_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBN6228114.1"
                     /translation="MKFGSVLIVVEDIEKSKRFYYDVLGLKVICDFGENVVLEGNISL
                     QEKKLWLEFINKGDSEVKFNGNDAELYFEEDNFDTFVERLSTMKDIDYVHLAIEHRWG
                     QRAIRFYDLDGHIIEVGETMSSVCRRFLDSGLSMDEVAKRMDVTVEYIESVLD"
BASE COUNT          843 a          298 c          198 g          706 t
ORIGIN      
        1 tatttgataa atttaaatta tcaaaattag attgctttat acaactattt tgttggaaac
       61 tatttattat ataaggagaa atcaataaaa aaatcagtgc ttcttataaa tgataataga
      121 aaagatttaa cagatttttt agaaaataat tttaaattag tttttggaga ttatataaat
      181 taaaaaattt agaacaaaat ctcactaaaa aattaaaaga aaaaggacaa atagcaaaat
      241 atacatttaa ggatataaaa acaactagcc ccaaaatgct cgaatatata gatttaacta
      301 agaaggtttc taaatctgac ttaagcatac ttgtcagagg tgaaagtaga accggtaaaa
      361 aactcatagc tcagtctata cataacaact caaatagaaa aaatcaaccc tttatagcag
      421 taaattgtac aactgttcct gaaaatttat tagaaaatga gatattcgga tatgacaaaa
      481 gaacttttac tggaggtcta aaagatggaa aataaggatt attttagcct gctaataatg
      541 gaataatatt cttagatgaa ataggctata tgcccttaga attacaaaca aaattactta
      601 aagttcttca agaaaaacag ataatgcctg ttggttctca caatataatt aatataggtg
      661 ttagaattat atctgctaca aataagaatt tggaacaaat tattgataat agccatttta
      721 gagagaattt atattatcgt ttaaatacaa ttccaataaa tatacctctt gtaagagaaa
      781 gaagatatat taattattat ggaagattta attaataaga aattagtaat cacaccagaa
      841 gcaaaaaaac ttatacaaaa ttatacgtgg aaggataata taagagaact tcaaaatgtt
      901 acatcttatt taaatattat gtgtgaagat attgtattag agaaagactt acctccaaac
      961 ttaaggtctt caagtaataa aacccatcat taaaattaaa atatagtaag aatgatatat
     1021 tttacaaaaa caaaaactgt aagtaaattt attttcacac aagtttactt acagccctca
     1081 cctcagtttt attttagcat aatccatcta gtctaaaact gattcaatat attcaacagt
     1141 tacatccatt cttttagcta cctcatccat tgaaagacca ctatctaaaa aacgtctaca
     1201 cacagatgac atagtttctc caacctcaat aatatgccca tctaaatcat aaaatctaat
     1261 tgctctttga ccccacctat gctctatagc tagatgaaca taatctatat ctttcattgt
     1321 actcaatctt tcaacaaaag tatcaaaatt atcttcctca aaatataact cagcatcatt
     1381 tccattgaac ttaacttcac tgtcaccctt attaataaac tctaaccaaa gctttttttc
     1441 ttgtaatgat atattccctt ctaatacaac attttctcca aaatcacaga ttacctttaa
     1501 tcccaaaaca tcataataaa acctttttga tttttcaata tcttcaacca ctattaaaac
     1561 acttccaaac ttcatatttc ctccttgata tacacatatt ttattttcat tacaaaagta
     1621 aaattaaact atacatattt caataagaca tgatataaac aagaaaattg taagtcttat
     1681 ttagatagtt gagttgatta tatcatataa tatttgcttt actcttgtaa aaaacagaca
     1741 tctctacact tttagattca aaattaaaat acatataaat ttaactaaaa aacttcctgt
     1801 ttttgtagat tatattatta tctaacttct aaaacaaaaa tactatttta gaatattgaa
     1861 atatctatat tcttttaatt tcaagtaaat attaatatac actaataata ttaatactct
     1921 ttttaaatat tacaagcata tgtattttac atgattaaag aaattattca agtattctaa
     1981 gtaataaaaa tatagttttt agtaacttct aatttaattt tcataatctt tctaattatt
     2041 gatat
//
LOCUS       DAFNNB010000265         2042 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0286, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000265 DAFNNB010000000
VERSION     DAFNNB010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0286"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..690)
                     /locus_tag="L3M60_003500"
     CDS             complement(<1..690)
                     /locus_tag="L3M60_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBN6228115.1"
                     /translation="MKKKVKFISFFILFILIISSIIVYKSLIFKDDIYVVSKKVSSNE
                     KTHAVEAEIKNEDNSHNKATKDIDKNFQNNKITIYISGAVNRPGIVTIEPDKRLYDAV
                     ELLGGTTKEADLNGVNLSVRLEDEQHYIIPKIGEAISVTSNDDSNKSNQKNESRSEPK
                     NKSNKNISNKSKVNINVATIEELDSLPGVGEATANKILQHREENGQFSSIEEIKNVNG
                     IGDKKYENIKDL"
     gene            770..>2042
                     /locus_tag="L3M60_003501"
     CDS             770..>2042
                     /locus_tag="L3M60_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HBN6228116.1"
                     /translation="MKRNLSLLLICLLIFTSFLGSSNISFADNEPSIVAKHAVLMDYE
                     SGKILYNKDGNSKLYPASTTKVWTACLVLKEVKDLNQVIEIKNLPQIDGSSMYLKEGE
                     SFTVKQLLNALLVHSANDAAFVLARYVGGGDVQKFIDLMNSEAKKIGATNTHFNNPHG
                     LPDPNHYTTAHDMALIAREAMNNDTFRQIVKTKSLKFEATKAYPYERYFVNTNKFLTS
                     HDKITYKGQPINIKYDIVDGIKTGYTDAAGKCLLSSAVKDGRRVIVAVFNSTNADLYL
                     DSRILIDYGFDNFKCATIVDKEKYTDTKKVLFTKQHELVYEPKNSYRIFLEKNESQGN
                     YDTKTELNKIDLPIKKGAKVGTLNIYNNGKLENSIDLIAKNNLDSSIPFLTGNNILMT
                     FVKIIAGILVLLVLFIITSNIKRKKRKKARSK"
BASE COUNT          709 a          276 c          280 g          777 t
ORIGIN      
        1 taaatctttt atattttcat attttttatc tccaatacca tttacatttt ttatttcctc
       61 gattgaacta aactgtccat tttcttctct gtgttgtaaa attttattgg ctgtagcttc
      121 tccaactcca ggtagagaat ccaattcttc tatagtcgct acatttatat ttacttttga
      181 tttgttagat atatttttat tagatttatt tttaggctca cttcttgact catttttttg
      241 atttgattta ttggaatcat cattgcttgt aacagatatt gcttcaccta ttttaggtat
      301 tatgtaatgt tgctcatctt ctaatcttac tgatagattg actccattta aatcggcttc
      361 ttttgtagtt cctcctagta attctacagc atcataaagt ctcttgtctg gctctatagt
      421 tacaatccct ggtctattta ctgctccact tatgtatata gttattttat tgttttgaaa
      481 atttttatct atatctttag ttgctttatt gtgactatta tcttcatttt taatttctgc
      541 ctctacagca tgtgtttttt cattagacga aactttttta cttacaacat aaatatcatc
      601 tttaaaaata agcgatttat aaacgattat tgatgatatt attaaaataa acaaaataaa
      661 aaatgaaata aattttacct tttttttcat atattaatca ctcctttctt ttaattattg
      721 acaaatatat gctatactaa aagatgaatt taaaatttgg aggctcacaa tgaaaagaaa
      781 tttatcactt ttattaatct gtttattgat atttacttca tttttaggaa gctctaatat
      841 ctcttttgca gataatgaac catctattgt tgctaaacat gcagttttaa tggactatga
      901 atctggcaaa atattatata ataaggatgg aaattctaag ctttatccag cttcaacaac
      961 taaagtatgg acagcttgtt tagttttaaa ggaggttaaa gatttaaatc aagtaataga
     1021 aattaaaaat ttacctcaaa tagatggttc tagtatgtat ctaaaagaag gagaatcttt
     1081 tactgtaaag cagttattaa atgctctttt agttcattct gctaatgatg ctgcttttgt
     1141 attagctaga tatgttggtg gtggcgatgt acaaaaattt attgacctta tgaactctga
     1201 ggcaaaaaaa attggagcta ctaatactca ttttaataat cctcatggat taccagaccc
     1261 aaatcactac acaacagctc atgatatggc tctcatagct agagaagcta tgaataatga
     1321 tacttttaga caaattgtaa aaactaagtc acttaaattt gaagcaacaa aagcatatcc
     1381 ttatgagaga tactttgtaa acactaataa gtttttgaca tcacatgata agattacata
     1441 caaaggtcaa cctataaata taaaatatga tatagttgat ggtataaaaa ctggatatac
     1501 tgatgctgct ggtaaatgtc ttttatctag tgctgtaaaa gatggcagaa gagttatcgt
     1561 tgctgtattt aattcaacta atgctgattt atatttagac tcacgtattc ttattgatta
     1621 tggatttgat aactttaagt gtgctactat tgttgataaa gaaaaataca cagatactaa
     1681 aaaagtatta tttacaaaac agcatgaatt ggtttatgaa cccaaaaata gttataggat
     1741 attcttagaa aaaaatgaat ctcaaggcaa ctatgatacc aaaacagagt taaataaaat
     1801 tgatttgcct ataaaaaaag gtgctaaggt tggtacttta aatatatata ataatggtaa
     1861 actggaaaac agtatagact taattgcaaa aaataatctt gatagcagta ttccattttt
     1921 gactggaaat aatattctaa tgacttttgt taagattatt gcaggtattc tagtactttt
     1981 agttttattt ataataactt caaatataaa gaggaaaaag agaaaaaaag ctagaagtaa
     2041 aa
//
LOCUS       DAFNNB010000266         2024 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0287, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000266 DAFNNB010000000
VERSION     DAFNNB010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0287"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     repeat_region   430..1578
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=430..458
                     /rpt_unit_seq="gttttatattaactatgtggtatgtaaat"
BASE COUNT          755 a          170 c          313 g          786 t
ORIGIN      
        1 aaaactgaat gttatttaaa gagagccttg attatatttt tagaagcttt ttatgcatat
       61 tatctaaaat atgagaatat ctattcatag ttatttttat atcagtatga cctaattttt
      121 tagagagaat ttttatatta aatccagtta gaagaagact tattagaata tccaatgtag
      181 taagcactag cagatattct taaattgata aattactcca ttttaatttt ataggttaaa
      241 ttttatgata taataaaaat atataaattt tgcagtgagc gatatttttg ataaagtagg
      301 gtttaacagt tgcaatgtaa ggcgttgagg gtgtatgata aatgttatca attgcactac
      361 tcatggttca ctgcaaattt aagaaagttg tatgtgtgta agtactgaaa atacttagtt
      421 tattttgggg ttttatatta actatgtggt atgtaaatct tgtagataat gcagaagaag
      481 aagaaaaaca gattgtttta tattaactat gtggtatgta aatgatgcta ccttattttt
      541 atctaatccc caaataaact gttttatatt aactatgtgg tatgtaaatt tatttcttaa
      601 ccagtcaaat aatctatttt gtcctggttt tatattaact atgtggtatg taaatatcat
      661 agtttattaa cacaggaaac gaggttagtt cgttttatat taactatgtg gtatgtaaat
      721 aactctttta aaaaagaata tacgattttc agagaaggtt ttatattaac tatgtggtat
      781 gtaaattatt aattgcttat ctattaattc ttttaatttt tcagttttat attaactatg
      841 tggtatgtaa attgaagggt tataaaaaaa ataatagcga aaagggcggt tttatattaa
      901 ctatgtggta tgtaaatcac gaaaaaatca gaaggggagg ggggttattt aaaagtttta
      961 tattaactat gtggtatgta aatttaaaac ttgaattatt tcaaaaagct aaacatcaag
     1021 gttttatatt aactatgtgg tatgtaaatt ttaattgccc ttcatagaat attatttcac
     1081 cttcttgttt tatattaact atgtggtatg taaattatga gaaagttgaa tttaaaggcg
     1141 aaatgaacat gggttttata ttaactatgt ggtatgtaaa ttctttatat tgatatccaa
     1201 gaataagcgc acgttcttgt tttatattaa ctatgtggta tgtaaatttg cttagcgcaa
     1261 taacttttga aaatactaca ggagtgtttt atattaacta tgtggtatgt aaattaagat
     1321 gtccaaatgc taagagagaa ttcttaggat agttttatat taactatgtg gtatgtaaat
     1381 aaaagaagtt ttgaaagtaa atgtgtaaat aataatagtt ttatattaac tatgtggtat
     1441 gtaaatgaag tcaacaattt ggctattggt taagtacaac tagcgtttta tattaactat
     1501 gtggtatgta aataaagaaa attgggtttt tgattaagaa cttgcgaatg ttttatatta
     1561 actatgtggt atgtaaattt gcaacaagta taggtaaaat actccaataa tttatatagc
     1621 attttctcct ttaaaatata attatttttt atcatttgta gtaagtaatt accagataac
     1681 attgacttta gtcttattga ttaaaatata aaagtagaat aattataaaa agtattgaaa
     1741 agtttataaa tatatagaat aaaacttaat gacaagatac tagatataaa aataattacc
     1801 ttataaatag attgaaattt gtgaatattc atactataat ttagatataa ggagatgctc
     1861 ttataaactt aaagggtttg tactacttgt actatcagta ttgataatat taaagtatat
     1921 ataccatttg tatttgttga aaaagatttt aatagtaata caatgttaaa caacacaaat
     1981 atcaaaattt aatattgata gaggtataaa cattataaaa ataa
//
LOCUS       DAFNNB010000267         1894 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0297, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000267 DAFNNB010000000
VERSION     DAFNNB010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0297"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            94..1887
                     /locus_tag="L3M60_003502"
     CDS             94..1887
                     /locus_tag="L3M60_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HBN6228117.1"
                     /translation="MTIKDRLSGLRKLMEEKNIDAYMIPSSDNHQSEYVGDYFKSREF
                     ISGFNGSAGTVIVTKDEAGLWTDGRYFIQAESQLEGSTIKLFKMGQEGCPTTDEYLYK
                     NIPEGGTLGFDGRVISAREGATLAEKLSKKGIKIEYQYDLIDGIWTDRPALSDSKAFL
                     LDVKYCGESFSSKLARLREKMSEKGTSTHVITTLDDIAWLFNIRGGDVKYNPVVLSYA
                     VITLKEVYLFVDESKLNEEILNELAKENVQIKPYSDVYEFVKNIDKAEKVLVDGTKLS
                     YTIYNNIPCEVEKVDEFNPVMFFKAQKNEIELENIRNSHIKDGVAFTKFMYWLKQNVG
                     KMEITEISATQKLEDLRREQEGFFEPSFNTIAAYKEHAAMMHYSATPESNYKLEAEGL
                     FLVDSGGQYYDGTTDITRTTVLGPISDELKLHFTSVARGMINLSKVKFLHGCRGYNLD
                     ILSRSCMWNMGIDYQCGTGHGIGFVLNVHEAPNGFRWRVVPERFDSAVLEEGMVTTNE
                     PGIYIEGSHGIRTENEIVVRKAEKNFYGQFMEFEVVTLAPIDLDGIVPELMNKDEKDY
                     LNWYHKLVYDKISPFLTDEEREWLKVYTRAI"
BASE COUNT          735 a          193 c          375 g          591 t
ORIGIN      
        1 atttatataa attatataaa aaatatttat gtaataaact ttacatactt atatatttag
       61 ttattcaaac tattattttt aggaggtctt gttgtgacta taaaagatag attaagtgga
      121 ttaagaaaac ttatggaaga aaaaaatata gacgcctata tgattccatc ttctgataat
      181 caccaaagtg aatatgtagg agattacttc aagtcaagag aatttatatc agggtttaat
      241 ggttcagcgg ggacagttat tgtgactaaa gacgaagctg gattatggac agatggaaga
      301 tattttatac aggcagagtc tcaattagaa ggaagtacaa taaaattatt caaaatgggg
      361 caagaaggtt gcccaactac agatgaatat ttatacaaaa atattcctga aggaggaact
      421 ttaggatttg atggtagagt tatatctgct agagaaggag ctacattagc tgaaaagtta
      481 tctaaaaaag gtataaagat agaatatcaa tatgatttaa tagatggtat atggacagat
      541 agacctgctt tatcagatag taaagcattt ttattggatg ttaaatactg tggagaaagt
      601 tttagctcaa aattagctag actaagagaa aaaatgagtg agaaaggtac ttctactcat
      661 gtaataacta ctcttgatga tatagcatgg ttattcaata taagaggtgg agatgttaaa
      721 tataatccag ttgtgctttc ttatgcggtg ataaccttaa aagaagttta tctatttgtt
      781 gatgaaagta agttaaatga agaaatatta aatgaactag ctaaagaaaa tgttcaaata
      841 aagccatata gtgatgtata tgaatttgta aaaaatatag acaaagctga aaaagtttta
      901 gtagatggaa ctaagcttag ttatacaatt tacaataata taccttgtga agttgagaaa
      961 gttgatgagt ttaatccagt aatgtttttt aaagctcaaa aaaatgagat agaattagaa
     1021 aatataagaa attcacatat aaaagatggt gtagctttca ctaaatttat gtactggtta
     1081 aagcaaaatg taggaaagat ggaaatcact gaaataagtg ctactcaaaa actagaggac
     1141 ttaagaagag agcaagaagg tttctttgag ccaagtttca atacaattgc tgcatataaa
     1201 gaacatgctg caatgatgca ttatagtgca actccagagt caaattataa attagaggca
     1261 gaaggattat ttttagtaga ttcaggtggg caatattatg atggaactac tgatataact
     1321 aggactacag ttttaggacc tataagtgat gaattaaaac tacactttac ttctgtagca
     1381 agaggaatga taaatctttc taaagttaaa ttcttgcatg gttgtagagg atataactta
     1441 gatatattat ctagaagttg catgtggaat atgggaattg attatcaatg tggaactgga
     1501 catggtatag gatttgtatt aaatgttcac gaagctccaa atggatttag atggagagta
     1561 gtacctgaaa gatttgatag tgcagttctt gaagaaggaa tggtaactac taacgaacca
     1621 ggtatatata tagaaggttc ccatggtata agaacagaga atgaaatcgt agttagaaaa
     1681 gctgaaaaaa atttctatgg tcaatttatg gaatttgagg ttgttacatt agctcctatt
     1741 gacttagatg gtatagtacc agaattaatg aataaggatg aaaaggatta tttaaactgg
     1801 tatcataagt tagtttatga taaaatatca cctttcttaa ctgatgaaga aagagaatgg
     1861 ttaaaagttt atactagagc tatatagttt gtac
//
LOCUS       DAFNNB010000268         1843 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0302, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000268 DAFNNB010000000
VERSION     DAFNNB010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0302"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1259
                     /locus_tag="L3M60_003503"
     CDS             <1..1259
                     /locus_tag="L3M60_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228118.1"
                     /translation="YINKLGDIEKRVVELTAKNVKKVYLRGIEILEEELVRLKNFYEL
                     MMSRKLDTNSIAYNDTLMELSSFFKSYIIKFEAQDIPVSIDYPLALDDMDIQGIYYVK
                     NYIESIDIENRFCNLFKKKDREKLFYDYGVTYKIDCDVILVNLFELIINNCIFSTILG
                     NEVTNLEISEYEYEFLEKQFKNICNNIAEDKVIVSEESNIIELIEVDDSFNTEADSNL
                     DYNDFEKITLILMEAIEVLVKRLRIEDEVVIRYIKKYEKRFIESVLSSIKSNTFKSMV
                     TVSKNKNEQLESYIVDDESKLDDESFRKIFNEILDSTSIIEKIRIIKENINAKKDFID
                     ILESECLFGEEYLMLFASLSELELAILGSVVFYEDIRMREINILEFLLNKKTETTLWK
                     IEYLKFMKRQSEDKIRSIEEHMNKIN"
     gene            1343..>1843
                     /locus_tag="L3M60_003504"
     CDS             1343..>1843
                     /locus_tag="L3M60_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HBN6228119.1"
                     /translation="MKEHIINNIEEIREELIDLSKKIWENPELAFEEKYASSIQKEYL
                     KSKGFKIEEVENLPTGFIASFGEGKPVIGILGEYDALPELSQCVSAERKPLVEGESGH
                     GCGHNLLGVAGVGAVVSTKKLIEENKFSGTIKYFGCPAEEEGGGKTVMCINGCFDDVD
                     CAFTWHP"
BASE COUNT          769 a          130 c          353 g          591 t
ORIGIN      
        1 cctatataaa taagcttgga gatatagaaa aaagagttgt ggagttgact gctaagaatg
       61 ttaaaaaagt gtatcttaga ggtattgaaa tcttagagga agaattagtt agactaaaaa
      121 atttttatga actaatgatg agtagaaaat tggatactaa ttcaatagcg tataatgata
      181 ccttgatgga gctatcaagt ttctttaaaa gttatattat aaaatttgaa gctcaggata
      241 ttccagtaag tatagattat ccattggcat tagatgatat ggatattcaa ggaatttatt
      301 atgtaaagaa ttatattgag agtatagata tagaaaatag attttgcaac ttatttaaga
      361 aaaaagatag agaaaaatta ttttatgatt atggggtgac ttataaaata gattgtgatg
      421 ttatacttgt aaatttattt gaacttatta taaataattg tattttttct acgattttag
      481 gaaatgaagt gactaattta gaaatttcag aatatgagta tgaattttta gaaaagcaat
      541 ttaaaaatat atgcaataat attgctgaag ataaagttat agtatctgaa gaaagtaata
      601 taattgaatt gattgaagta gatgatagtt ttaatactga agcagatagt aatttggatt
      661 ataatgattt tgaaaaaata accttaatac ttatggaagc aattgaagtt ttggtaaaaa
      721 gattaagaat agaagatgaa gttgtaataa gatatattaa aaaatatgaa aaaagattta
      781 ttgaatctgt gctaagttca ataaaaagta atacttttaa aagtatggta actgtaagta
      841 aaaataaaaa tgaacaatta gaaagttata ttgtcgatga tgaaagtaaa ttggatgatg
      901 agtcgtttag aaagatattc aatgagatat tagacagtac gtctataatt gaaaaaataa
      961 gaataataaa ggaaaatatc aatgctaaaa aagattttat cgatatcctc gaatctgaat
     1021 gtttatttgg agaagagtat ttaatgttgt ttgcttcatt aagtgaatta gaattagcaa
     1081 tattgggtag tgtagtgttt tatgaagata ttagaatgag agaaattaat atattagaat
     1141 ttctattaaa taagaaaact gaaactactt tatggaaaat agagtacctt aaatttatga
     1201 agagacaaag tgaagataag atacgctcca tagaagaaca tatgaataaa attaattaac
     1261 aaaaaattgt gaataaatag aatgttagta tataatataa tttatgaagc atatcaaaaa
     1321 tacatatatt tggaggaaaa ttatgaaaga gcatatcata aataatattg aagaaataag
     1381 agaagaatta atagatttat ctaagaaaat atgggaaaat ccagaattag cttttgaaga
     1441 aaagtatgca agtagcatcc aaaaagaata cttaaagtca aaaggattta aaattgaaga
     1501 agtagaaaat ttacctactg gatttatagc aagttttgga gagggtaaac ctgtgattgg
     1561 catacttgga gaatatgatg ctttaccaga gctatcacaa tgtgtgagtg cagaaagaaa
     1621 gccattggta gaaggagaat ctggacatgg atgtgggcat aacctattgg gtgtagcggg
     1681 tgtaggagct gttgtttcta caaaaaaatt aatagaagaa aataaattta gtggaacaat
     1741 aaaatatttt ggttgtcctg ctgaagaaga aggtggagga aagacagtta tgtgtataaa
     1801 tggatgtttt gatgatgtgg attgtgcatt tacatggcat cct
//
LOCUS       DAFNNB010000269         1835 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0303, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000269 DAFNNB010000000
VERSION     DAFNNB010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0303"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..332)
                     /locus_tag="L3M60_003505"
     CDS             complement(<1..332)
                     /locus_tag="L3M60_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6228120.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMGFATESNAMENNADLNINLEKK
                     SIVLGSKSKVSVKFKEKPDANSIILKYKCYDMPLDTTLNYNQSTESYEGTINYNKDPE
                     YLNVWELQG"
     gene            complement(818..1780)
                     /locus_tag="L3M60_003506"
     CDS             complement(818..1780)
                     /locus_tag="L3M60_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6228121.1"
                     /translation="MISTNVINKVNGITKDSVTNNRVYGADRHETNANVIKKFYTDDE
                     LEAVLVAKSDVLVDALAAGPLAANLKSPILITPKTYVSAYHKENLEAKSANKVYKIGG
                     GLTSKVMSSIASSLSKHNTTPTDPGTSGGKTVMIDPGHGGSDTGTTGKPLGGIKEKDY
                     TLNTSLATTEYLRSKGFNVIMTRDTDKTLSLGNRTALSNSLRPDLFTSIHYNASDTTG
                     NGVEVFYKLKDKDGGTTKTVATNILNRILEKFNLKNRGAKTRTLSTDPTKDYLYVLRN
                     NDMPAVLVECAFLDNEKDMSLLNTSDKVKEMGTQIGKGIEDSLK"
BASE COUNT          573 a          306 c          215 g          741 t
ORIGIN      
        1 ccttgtagtt cccaaacatt tagatattct gggtctttat tataattaat agttccctca
       61 tacgactcag ttgattgatt gtagtttaga gttgtgtcta atggcatatc ataacattta
      121 tattttaata taatgctatt tgcatctggt ttttctttaa atttgacgct aacttttgat
      181 ttactaccta aaacgatact ttttttctct aagtttatat ttaaatcagc attattttcc
      241 atagcattag attcagtagc aaatcccatt gtaggtgcaa cgattgccat tgaaagaatc
      301 cctgttgtta ataattttgt atttcttctc atattaatct ccttatttaa cataaatttc
      361 tactttgttt tttaaccttt taacttttga aaatatggca aagtgtatct atctaaaaat
      421 ataaataagt acataaagat aaacttataa aactcaaaaa ataaaaaaat taaatatatt
      481 taatacattt aattatttat actatagcat ttattaccat aaaaatccac aaaaaaacac
      541 aataaagtat agtaattgaa cttttactgt gtatcacaaa tgaatacgcc aactcttgac
      601 gattagctat aaccttaaaa tatgtaaatt ctttatataa cataaattct tttacaatta
      661 actacaaaaa gactatatat acatcactta gcacttatat gcacaatgta tatatagtct
      721 ttattactat tgtaattaca ttcttatctt atagctttag ttaaaattta aaattgcaaa
      781 ttaatttatt ttaaattttc tatgctctca ttgttggcta ttttaatgaa tcttctattc
      841 ctttacctat ttgtgtaccc atttctttta ctttatctga tgtattaagt aaactcatat
      901 ctttctcatt atctaaaaat gcacattcta caagtacagc tggcatatca ttatttctta
      961 aaacatataa ataatctttt gtagggtcag ttgatagtgt tcttgttttt gcacctctat
     1021 tttttagatt aaatttctct aaaattctat ttaatatatt tgtagctaca gtcttagtag
     1081 ttcctccatc tttgtcctta agcttataaa atacttctac accattccca gttgtatcag
     1141 atgcattata gtgtatactt gtaaataaat ctggtctcaa agagttagat agagcagttc
     1201 tatttccaag agataaagtt ttatctgtgt ctcttgtcat tattacattg aacccctttg
     1261 aacgtaaata ttcagttgtc gcaagtgaag tatttaaggt atagtctttt tctttaatac
     1321 ctcctaatgg tttacctgtt gttccagtat ccgaaccgcc atgccctggg tcaatcataa
     1381 ctgtcttccc accactagtt cctgggtctg ttggagttgt attgtgttta gataatgatg
     1441 atgctataga actcattacc ttagaagtca atcctcctcc tattttatat actttattgg
     1501 ctgatttagc ttctaagttt tctttatggt atgcagatac atacgtcttt ggtgttataa
     1561 gtattggaga ttttaagtta gcagccaatg gacctgctgc taaagcatca acaagtacat
     1621 ctgatttagc tactaaaaca gcttccaact catcatctgt atagaatttt tttattacat
     1681 ttgcatttgt ttcgtgtcta tctgctccgt atactctatt attagtaaca ctatccttag
     1741 ttattccatt taccttattt ataacatttg ttgatatcat ttgaggacca cctataaaat
     1801 aagcactttg taaatcctca ctctttaacc attta
//
LOCUS       DAFNNB010000270         1824 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0304, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000270 DAFNNB010000000
VERSION     DAFNNB010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0304"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            795..1262
                     /locus_tag="L3M60_003507"
     CDS             795..1262
                     /locus_tag="L3M60_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBN6228122.1"
                     /translation="MITKFFSNILSVFLYFLLSLLGVAGIIFLCMIPMFLYDFSSDKV
                     IALITRILYFSDYFVVILFLIFIINSTKYSPFILENVKRFKMMGCCLLVNTIIECVVG
                     YKSNTSLIMIFGDNSGGISPLMVISFIFALMCFVIAETFDKAIKIKKDNDLTI"
     gene            1273..1488
                     /locus_tag="L3M60_003508"
     CDS             1273..1488
                     /locus_tag="L3M60_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228123.1"
                     /translation="MAIVVNLDVMMAKRKISLKDLAEKIDITNANLSILKTGKAKAIR
                     FTTLNEICKALDCQPGDILEYVEDENE"
     gene            1631..1804
                     /locus_tag="L3M60_003509"
     CDS             1631..1804
                     /locus_tag="L3M60_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003424268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HBN6228124.1"
                     /translation="MKRKLSTYFKGYVLGLCIAFLLFSFILKKLMISNIIGIVIGTGI
                     AFLLICLFNKKRK"
BASE COUNT          696 a          191 c          199 g          738 t
ORIGIN      
        1 attatataat ttttatttat cactctcaat aaattatagt tatctacaaa attaaatctt
       61 taatacatta ttaatatata ttgtgatatt aatattacag tactcaataa aatatctatt
      121 atataaatat ccactcttat gtttgaaatc tatcatgtag ttttatcctt ctcatctttt
      181 atatcaataa tttttttaaa taaaattgac aaaactaaag taaaaaatcc tattgataat
      241 gaaacaaata atactataac ttctgatatt atagtaattg catataaata gacataataa
      301 caatatataa tattgtcact acaaaattat atataaaaat ctcaaatcta actaaatgta
      361 catatatgta ttttttaagt ttttaggtat ttcttttgaa aattatttat tgattgctac
      421 caattacata agctttttaa tataaacaat gatacaatat atgggacagc acatatgtat
      481 atagctactt atattattta tattacagtt ggcatattcg tatttgtaat gcttatacca
      541 ctcttaaaat acatcatcag tactaatggt attaaaataa tatataataa agttaaaatt
      601 attccaatta gtataatatc atttaatatt ttacaactca tattcttttt tatacaaatt
      661 taccacctta tacattctta tctttagcgt atattataat ggttttatat attatatcaa
      721 taatttatta ttgtaattca ataaattata tattataatc aataatatac tttatataaa
      781 ggagatgaaa ttttatgatt acaaaatttt ttagtaatat attatcagta tttctatatt
      841 ttctattatc acttttaggt gttgctggta taatattctt atgtatgata cctatgtttt
      901 tatatgattt tagttctgac aaagtaattg ctttaattac aagaatacta tatttttctg
      961 actactttgt agtcatctta ttcttgatat ttataattaa tagcactaaa tactcccctt
     1021 ttatacttga aaatgtaaaa agatttaaaa tgatgggatg ttgtttatta gtaaatacta
     1081 taattgaatg tgtagttggt tataaatcaa atactagtct tataatgata tttggagaca
     1141 atagtggtgg tatttcacca cttatggtta tttcttttat atttgcatta atgtgttttg
     1201 taattgctga aacgtttgat aaagctataa aaataaaaaa agataatgac ttgacgatat
     1261 agaggtgatg ttatggcaat tgtagttaat ctagatgtaa tgatggcaaa aaggaaaatc
     1321 tcacttaaag atttggcaga aaaaatagat attacaaatg caaatttatc gattttaaaa
     1381 actggaaaag caaaagctat tcgatttact actctaaatg aaatttgcaa agctttagat
     1441 tgtcagccag gcgatatact tgaatatgta gaagatgaaa atgaataaaa aagagtgttt
     1501 aaaaacactc ttttttatta caatatttac ttactctatc taatagttgt aatcttttag
     1561 atactctttg gagttacaac actgctatac tctaattaaa taagtcaaat ctatacaagt
     1621 tggtgattat atgaaaagaa aactttcaac atattttaaa ggttatgttt taggtttatg
     1681 catagcattt ttattatttt cttttatatt aaaaaaatta atgatatcaa atatcattgg
     1741 aatagttatt ggaactggaa ttgcattttt attgatttgc ctatttaata aaaaaagaaa
     1801 ataacattct ataagaaggt tttt
//
LOCUS       DAFNNB010000271         1771 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0314, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000271 DAFNNB010000000
VERSION     DAFNNB010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0314"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(239..1567)
                     /locus_tag="L3M60_003510"
     CDS             complement(239..1567)
                     /locus_tag="L3M60_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBN6228125.1"
                     /translation="MESNELKKTLGVSAALSTVVGSVIGAGVFFKPQAVYTLTGGAPG
                     LGILAWLIAGIITITAGLTAAEVSVAIPKTGGMMVYIKEIYGEKLGFLTGWMQIVLFY
                     PGMMAALGVIFGEQASALIGSPSLLLPIAIGIIVIVAGLNMLGSKTGGVIQTVSTICK
                     LIPLILIMIVGFIKGGGNNPILTPMVGEGLSLGSVLGQVLIAILFAFDGWMNVGTLAG
                     EMKNPGKDLPKAIIGGLSVVMAVYFIINLAYLWVLPASELANYASPASAVAEVIFGSM
                     GGKIISVGILISVFGALNGFLLTGSRVAYTLATDKTLPKYSIFSKLNSAQVPANAIAL
                     VSVIASIYALSGQFNLLTDLAVFATWIFYVLTFIGVMKLRKTHPNIPREYKVPLYPIV
                     PIIAIASGIFVVVNQLCFAGMKTTMISIGGLVITAIGLPVYAYMTRGIKR"
BASE COUNT          643 a          329 c          197 g          602 t
ORIGIN      
        1 aatactttaa aataaagtat tttaaagcaa ataatttttt agatgtttaa ttttgtaatc
       61 caaaaactaa aaataaaata aataccttaa gataagcttt ttagttcatg aaacactctt
      121 tttttataaa atcaaaccta tctttgtcat tttaacaata aaaaagagac ttctctctat
      181 tttgagacag tctcttttat tttttaaata attataaagc atttgtaaac tacttaattt
      241 atctttttat tcctctagtc atataagcat acactggtaa acctatagct gttattacaa
      301 gtcctcctat agatatcata gtagttttca tacctgcaaa acacaattga ttaactacaa
      361 caaatatacc acttgctata gctattatcg gaacaatagg atataatggc actttatact
      421 ctcttggtat atttggatga gtctttctaa gtttcattac ccctataaaa gttaatacat
      481 agaatatcca agtagcaaat acagctaagt cagttaataa attaaattga cctgacaaag
      541 catatataga tgcaataaca gatactaatg ctattgcatt tgctggaact tgagcagaat
      601 ttaatttaga gaatatacta tattttggta aagtcttatc tgtagctaaa gtgtatgcca
      661 cccttgaacc agttagtaaa aatccattta atgctccaaa cactgaaatt aatataccta
      721 cagatattat tttcccaccc attgaaccaa atataacttc agcaactgct gatgcaggag
      781 aagcataatt tgctaattca ctagccggta atacccaaag atatgctaaa tttattataa
      841 agtaaacagc cataacaact gataatccac ctattatagc ttttggaagg tcttttccag
      901 gattcttcat ttctccagcc aaagtaccaa cattcatcca tccatcaaat gcaaataata
      961 tagcaattaa aacttgacct aatacactac ctagacttaa tccctcacca accataggcg
     1021 taagtattgg attatttcca ccacctttaa taaatccaac aatcattatt aaaataagtg
     1081 gtattaattt acaaattgta gaaacagttt gtataactcc tccagttttt gaacccagca
     1141 tattaagccc tgcaactatt actataattc caattgctat tggaagtaag agtgatggac
     1201 taccaattag tgctgatgct tgttctccga atataacacc taatgctgcc atcatccctg
     1261 gataaaataa tacaatttgc atccatccag ttaaaaaacc taatttttct ccataaattt
     1321 cttttatata taccatcatt ccaccagttt ttggtatcgc aactgatacc tctgcagctg
     1381 taagaccagc tgttatagtt atgattccag ctattaacca tgccaatata ccaagacctg
     1441 gagctcctcc agttaatgta taaactgctt gtggtttaaa aaatacaccc gctcctataa
     1501 cagaaccaac aacagttgat aatgccgctg atacgccaag agtctttttc agttcattac
     1561 tttccatttt cttcctcctc attaatccta tttaatttta atttcatagt taaataagca
     1621 tatattatgc caatactata gaaaaatagc tttattattt attaaaataa aataaactct
     1681 acttagcttt tgtaattttt tgcttttaat caagttcaaa tataaaaaaa ttataaaaaa
     1741 caaaaatcta aattaatgat acttattaaa a
//
LOCUS       DAFNNB010000272         1762 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0315, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000272 DAFNNB010000000
VERSION     DAFNNB010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0315"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            121..1110
                     /locus_tag="L3M60_003511"
     CDS             121..1110
                     /locus_tag="L3M60_003511"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBN6228126.1"
                     /translation="MFENLPKIDLHCHLDGSVRVETMLDIAIKEKIDLPSNNMDEIKK
                     LAKVSFNCTSLDEYLEKFDLPLKVMQSKENLKRITFELLEDASRENVKYIEIRFAPLL
                     HTQKGMSVKNIIEGIIEGIREAESIYDIKGNLILGCMRTMTSKEALLVIEEGKSFVNK
                     GVVAVDLCGPEKEGFCKEYKDAFKLAREYGYKVTIHAGEAASGENVLDAINILKADRI
                     GHGVKIKDNKKAYNLVKDKKILLELCPTSNVQTKTVDSYETHPFYTFYKDNLHVSINT
                     DNRTVSDISLSSELNVIFDTFKLGIEDYKIIYRNAVEASFADKETKEYLNSLI"
     gene            1182..1748
                     /locus_tag="L3M60_003512"
                     /pseudo
     CDS             1182..1748
                     /locus_tag="L3M60_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
BASE COUNT          748 a          159 c          305 g          550 t
ORIGIN      
        1 taaaatattt taatgagatg tgactttttt agaatgaaaa atcgtagtat attgatataa
       61 tagaaataag acttaaaaat ctgttgatag tattatttat aagtacagta ggaggataaa
      121 atgtttgaaa atttacctaa gatagattta cattgccact tagatggaag tgttagagta
      181 gaaaccatgc ttgatatagc tattaaagag aaaatagatt taccttcaaa taatatggat
      241 gaaataaaaa aattagcaaa ggtttctttt aattgtactt cacttgatga atatttagaa
      301 aaatttgatt tacctttaaa agtaatgcaa tctaaggaaa atttaaaaag aattactttt
      361 gaacttttgg aagatgcaag tagagaaaat gtaaaatata ttgagattag atttgcacca
      421 ttacttcaca cccaaaaagg tatgagtgtt aaaaatataa ttgagggtat aattgaagga
      481 attagagaag ctgaaagtat ttatgatata aaaggcaatt taatattagg ttgtatgaga
      541 acaatgacct caaaagaggc attattagta attgaagaag ggaaatcatt tgtaaataaa
      601 ggtgttgtag cagtagactt atgtggtcca gagaaagagg gattttgtaa ggaatataaa
      661 gatgctttca aattagcaag agaatatggt tataaagtta caatacatgc aggagaagct
      721 gcaagtggag aaaatgtatt ggatgctata aatattttaa aggcggatag gattggtcat
      781 ggggtaaaaa ttaaagacaa taaaaaggct tataatttag ttaaagataa aaaaatattg
      841 cttgaattat gtccaactag taatgtacaa actaagacag tagattctta tgaaacacat
      901 ccattttata ctttttataa agataattta catgtaagta taaatactga taatagaact
      961 gtatcagata taagtttaag ttcagaacta aatgttatat ttgatacttt taaactagga
     1021 atagaagact ataaaataat ttatagaaat gcagtagaag ctagttttgc agacaaagaa
     1081 actaaagagt atttgaatag tttaatttaa tagataaaaa tatataaact ttaaattaag
     1141 ctattaaatt gacaaaaata cattgtatta ggagaaaaaa tgtgaaagat tataaagatg
     1201 ttgtttatat taaactagtc aaaaaagaac ttgaagaatg gaagaaaaat atattaaaga
     1261 aaccttctat atttgacaaa acatcaaagg ggatacagaa taagtttaat ggagtattgc
     1321 cagataaata tcatgaaata attacttctt ctataaaagt aatgacaaaa actgtattgt
     1381 ttggatataa atatgccaca aagccaccac ttagaaagat gtcattagaa gaaagagaaa
     1441 aattagtttt agaaaaagct aaaacataca agaccacagc aatgattgaa ggagcaggaa
     1501 caggggcagg aggaatcctc ttaggtttat ctgattttcc tttactttta ggtattaaag
     1561 taaaatttct atatgatgta gcttcaatat atggatatga tgttaaagat tatagagaaa
     1621 ggatttatat attaaacata ttacagttgg ctttttctag ccaacagcat ataaataaaa
     1681 cttttaaatt gataaataac tgggatgaat atgtaagtag tctaccagaa gacatcaatg
     1741 attttgatta aaaaatagct ac
//
LOCUS       DAFNNB010000273         1754 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0316, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000273 DAFNNB010000000
VERSION     DAFNNB010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0316"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            67..324
                     /locus_tag="L3M60_003513"
     CDS             67..324
                     /locus_tag="L3M60_003513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228127.1"
                     /translation="MHNVDCNDKSVYIGNNELDRSKYLKENELLALFGDGASIDMKHN
                     SKLDMQISNPNLKSVNIYPYTPFVGLRGTLYIIEKILNIQY"
     gene            556..747
                     /locus_tag="L3M60_003514"
     CDS             556..747
                     /locus_tag="L3M60_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228128.1"
                     /translation="MERLMELIERKFLKDVELLELEDIENVIECKMTKPYNAHYNEYL
                     VKVKNGDEVEEYFVYLKHR"
     gene            complement(835..1647)
                     /locus_tag="L3M60_003515"
     CDS             complement(835..1647)
                     /locus_tag="L3M60_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HBN6228129.1"
                     /translation="MADCNSCPSKGNCNSQSNCSIENNPNNKFGKIIGVMSGKGGVGK
                     STVTALLANKLNKMGYKVGILDSDITGPSIPRLMGVKNVKAYSDGSYIYPVENSNNIK
                     VMSINLMIDDENEPVVWRGPLLGGVVKQFYTDVLWEELDYLLIDMPPGTGDVALTVMQ
                     SIPISGIVMVSVPQDLVSMIVSKAVNMAKKMNINVLGVIENMSYIQCPDCSKKIKLFE
                     GESTEKFLDDLDLELLGELPMTKEIIDITHNRVTEISDDLDSILTNVVEKIK"
BASE COUNT          678 a          232 c          231 g          613 t
ORIGIN      
        1 tatgcaaaat tctatgaagc aggaaccctg tgactttagt catgggaggt tcagagagtt
       61 aatgtattgc ataatgtaga ttgtaatgat aaatctgtgt atattgggaa caatgaactg
      121 gatagaagta aatatttaaa agaaaatgag ttattagcat tatttggaga tggtgctagt
      181 atcgatatga aacataattc aaagttggat atgcagataa gtaatccaaa cctaaaaagt
      241 gtaaatatat atccatacac tccttttgta ggattaagag gaactcttta tataattgaa
      301 aaaatactaa atatacagta ctaaatatat aaaaaatatt atttaaatac atatacaata
      361 cataaaaaag tttttattaa gaaaatgttg ataaaaaaaa ggtacattta ttaaaaaatc
      421 aatctctact tgctaaaata attatgaatt atgttagaaa ctgtaatata ttggatataa
      481 tataataaac gggtaaactt tggattaaga ggattatata gtttatctta attatagggg
      541 aagtgaggat atattatgga aagattaatg gaactcatag aaagaaaatt tttaaaggat
      601 gtagaacttt tagaattaga agacatagaa aatgtaatag aatgcaaaat gactaagcca
      661 tataatgctc attataatga atacttagta aaagtaaaaa atggagatga agtagaagaa
      721 tattttgtgt atttaaaaca tagataaaca taaaagaggt ttatatttat ttgtatgatt
      781 ataaaataaa tataagcctc ttttttgata aatataaaat tataaaattt ttatttattt
      841 aattttttca acaacattag tcaatattga atctaaatca tcactaattt ctgtaactct
      901 attatgagta atatctataa tttctttagt cataggaagt tctcctaaaa gctctaagtc
      961 taaatcatct aaaaactttt cagtagattc tccttcaaat aacttgattt ttttagaaca
     1021 atcagggcat tggatataac tcatattttc aataactcca aggacattta tgttcatttt
     1081 tttagccata ttaacagctt ttgatacaat catagatacc aaatcttgag gtactgaaac
     1141 cataactatt ccacttatag gaatagattg cataactgtt aaggcaacat ctccagtacc
     1201 aggaggcata tctattaata aatagtctaa ttcttcccaa agtacatcag tataaaattg
     1261 ctttacaact cctccaagta aaggtcctct ccaaacaaca ggttcattct catcatctat
     1321 cataagattt atagacatta cttttatgtt atttgagttt tctacagggt aaatataaga
     1381 gccatcagaa taagctttta cattttttac acccataagt ctaggaatac ttggtccagt
     1441 tatatctgaa tctaatatac caaccttata tcccattttg tttagcttat tagcaagtaa
     1501 agcagttaca gttgattttc caactccacc cttcccactc ataacaccaa ttatttttcc
     1561 aaatttattg ttagggttat tttcaataga acaatttgat tgactattac aatttccctt
     1621 tgatggacat gaattacaat ctgccatatc tattacctcc aaactttaat atttattaga
     1681 ttatttgtaa cctacatata ctattttagt acaattgaac cataaagtca ttatttaaaa
     1741 gtaacttaga agat
//
LOCUS       DAFNNB010000274         1742 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0317, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000274 DAFNNB010000000
VERSION     DAFNNB010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1742
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0317"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..781
                     /locus_tag="L3M60_003516"
     CDS             <1..781
                     /locus_tag="L3M60_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN6228130.1"
                     /translation="KEIRIIPKHKARFFEIQYCYEIPKSEEVSNQKNALAIDLGLENL
                     CTCVTNLGDSFIIDGKRLKSVNQWANKQNDKIEALKLKKNITAITRKQFKVWNKRNNQ
                     VKDYVNKTCFYIIDYCIKNNIGNLIVGYNAKLEKSDNMERKIYRNFIRIPFGEIKGKL
                     EYLCKIKNINFIRQEESYTSKSDFFANDFLPDINVDNVKKYDFKGERITRGQYKSSVG
                     IIINADVNASLNILKKSQVVNLSNLQNNPQILKQPQRVRIS"
     gene            964..1686
                     /gene="yqeC"
                     /locus_tag="L3M60_003517"
     CDS             964..1686
                     /gene="yqeC"
                     /locus_tag="L3M60_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBN6228131.1"
                     /translation="MKLSNIIEKNEIITVVGAGGKTSFINYFANFYRDKLKVLLTTTT
                     KIYVPNDYDNIITTIDGTIIPSICHGIIVCGSHINNENKLVSIDSSILDEIVDKFDLV
                     LIEGDGSKRKKLKGWNTKEPVVYHKTTKTIGILDITSFGMDINEENIHRVEMFKKIAN
                     LNTKSINSSSTVSIENLKNIVLNPNGLFKNYSGKRVLFINKVENEKYKNLAIKLIKNI
                     KECESEIEIFYGSVKQKFCARY"
BASE COUNT          779 a          154 c          270 g          539 t
ORIGIN      
        1 taaagaaata agaataatac ctaaacataa agctaggttc tttgaaattc agtattgtta
       61 tgaaataccg aaatcagagg aagtttcaaa tcaaaaaaat gcactagcta tagatttagg
      121 tttagaaaat ttatgtacat gtgttaccaa tttaggtgat agttttatca tagatggcaa
      181 aaggcttaaa tctgtgaatc agtgggcaaa caaacagaat gataaaatag aggccttaaa
      241 attaaaaaag aatataactg caataacaag aaaacaattt aaagtttgga ataagagaaa
      301 taatcaagtc aaagactatg taaataaaac ttgtttttac ataattgatt actgtataaa
      361 aaataatatt ggtaatttaa ttgtaggata taatgctaag ttagaaaaaa gtgataatat
      421 ggagagaaaa atatatagaa attttataag aatccctttt ggagagataa aaggaaaatt
      481 agaatatcta tgtaaaataa aaaatatcaa ttttattagg caagaagaaa gctatactag
      541 taaatctgat ttttttgcca atgattttct acctgatatc aatgtggaca atgtgaaaaa
      601 atatgacttt aaaggggaaa gaataacacg tggacagtat aaatccagtg ttggaatcat
      661 tataaatgca gatgtaaatg cgagtcttaa tatattaaaa aaatcacaag tagttaattt
      721 atctaatttg cagaataatc ctcaaatatt aaagcaacca cagagagtaa gaatatcata
      781 attatattta aaagataaac tgatagcgta agcttacagc ttagcggcga attggatccg
      841 ccttgttgtt tacaacagaa tctatttact ttcgagttta tggaatttca aaaatcactt
      901 ttactaaaaa ggtctagctg taaaaattaa tagattagaa aactggttat aagaggtgaa
      961 agtgtgaaac tatcaaatat aatagaaaag aatgaaataa taactgtagt tggagcagga
     1021 ggaaaaactt catttataaa ctattttgca aatttttata gagataaatt gaaagttcta
     1081 cttacaacaa caactaaaat atatgtacca aatgattatg acaatataat tactacaata
     1141 gatggaacta ttattccatc tatttgtcat ggaataatag tatgtggtag tcatataaat
     1201 aatgaaaata aattagtaag tattgattca tctattttgg atgaaattgt agacaaattt
     1261 gatttagttt taatagaagg agatggttca aagagaaaaa aacttaaagg ttggaatact
     1321 aaagaaccag tagtatatca taaaactaca aaaactatag gtatattaga tataacatca
     1381 tttggaatgg atattaatga agaaaatata cacagagtag aaatgttcaa aaaaatagca
     1441 aatttaaata caaagagtat taatagtagc tctacagtaa gtattgaaaa cttaaaaaat
     1501 atagtattaa atccaaatgg attatttaaa aattattctg gaaaaagagt attatttatt
     1561 aataaagttg aaaatgaaaa atataaaaat ttagctataa aattaataaa aaatattaaa
     1621 gaatgtgaaa gtgaaataga gatattttat ggaagtgtaa aacaaaaatt ttgtgcaaga
     1681 tattgaagta ttaaaatagt ttatatgata ttatatatat agataatatc aatagagata
     1741 tt
//
LOCUS       DAFNNB010000275         1682 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0324, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000275 DAFNNB010000000
VERSION     DAFNNB010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0324"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            230..1522
                     /gene="brnQ"
                     /locus_tag="L3M60_003518"
     CDS             230..1522
                     /gene="brnQ"
                     /locus_tag="L3M60_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HBN6228132.1"
                     /translation="MGEKQINFKDVIVIGFALFAMFFGAGNLIFPPYLGVLSGSSWLV
                     AFIGFLFADGGLALLAVIAATKFNGDTSKMFARAGKGLSVALGCAMVICIGPLLAIPR
                     TAATTYEMGILPTIGSGISPVIFSIVFFALVLVLTIRPSKVVDIVGSILTPALLIALA
                     VLIVKGIVSPLGEIRDSSLIQNVFAEGITQGYQTMDALAASVFASIIIMSVIAKGYTG
                     DKEKMKATISAGVIAVIGMALVYGGLCYLGATVSQQYGQDVQQTALIVAITAALLGTT
                     GKILLAIIVALACLTTAIGLSSAAGQYFSTLTDGKLKYEHIVIVVCVFSAIISNFGVS
                     TIIQFSSPILSMVYPATITLVILALFSNKIKNDNVFKCAAYMALLVSVLTVATSFGVK
                     VPLVNNLPFASLGFNWIVPVVIAGIIGNFIPSKSSKTA"
BASE COUNT          603 a          207 c          298 g          574 t
ORIGIN      
        1 tagataaaaa acaaaaaacc aactatgttt aaagtataca cgttgatttt gtttaattaa
       61 gaaagttgta ttaaatattg tgaagatttt ataaattatg aaacataatt gacaaattta
      121 agtcaaaaat gtataattaa ataaaatctc gtcaaaaggt tttcatttaa gaagagattg
      181 gacttatttt ttagtaaaat gtagtaaaat tcaaatgggg gtgctttaaa tgggtgagaa
      241 acaaatcaat tttaaagatg taattgtaat tggatttgca ctatttgcaa tgttttttgg
      301 tgcagggaac ttaatcttcc ctccatattt aggagtttta tctggaagta gctggctagt
      361 agctttcata ggattccttt ttgcagatgg tggtttggca ttattagcag ttatagcagc
      421 aactaagttc aatggagaca catcaaagat gttcgccaga gcaggcaaag gattaagtgt
      481 tgcattagga tgtgcaatgg taatctgtat agggccactt ttagcaatac ctagaacagc
      541 agcaacaact tatgaaatgg gtatattacc tacaataggt tctggaataa gtccagtgat
      601 attctctata gtgttctttg ctttagtatt ggttttaaca ataagaccat caaaagttgt
      661 agatatagtt gggtcaatat taactccagc tttacttata gctttagcag tattaatagt
      721 taaaggtatc gtatctccat taggagaaat aagagacagt tctttaatac aaaatgtatt
      781 tgcagaaggt ataactcaag gttatcaaac aatggatgca ttggcagcta gtgtatttgc
      841 atcaattata atcatgtcag taatagctaa aggttataca ggtgataaag aaaagatgaa
      901 agccacaata agtgcaggtg tgattgcagt aataggaatg gcactagttt atggtggatt
      961 atgttatctt ggagcaactg tgtctcaaca atatggacaa gatgttcaac aaacagcttt
     1021 aatagttgca ataacagcag ctttacttgg aactacaggt aaaattttat tagctattat
     1081 agttgcatta gcatgtttaa caacagctat aggtctttct tcagcagctg gtcaatattt
     1141 ctcaacactt acagatggaa aattaaaata tgagcatata gttatagtag tttgtgtatt
     1201 tagtgcaata atatcaaact ttggagtaag tacaataata caattctctt ctcctatact
     1261 aagtatggta tatccagcaa ctataactct agttatacta gcattattta gtaataaaat
     1321 taaaaatgat aacgtgttca aatgtgctgc atatatggca ttattagtaa gtgtattaac
     1381 agttgcaaca agttttggtg tgaaagttcc tctagttaat aacttaccat ttgcttcatt
     1441 aggatttaac tggatagttc cagttgtaat agcaggaata ataggaaact ttattccatc
     1501 taaaagtagc aaaactgcat aattataaaa actaattgat aattatggaa ttgcctttaa
     1561 attatatagg caattccttt tatatttaaa agaattatta aattattgta gataaaagac
     1621 tattattaat cctatttaat aaattgaaaa atttagtttt ggagtagtac aatttataaa
     1681 ga
//
LOCUS       DAFNNB010000276         1644 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0328, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000276 DAFNNB010000000
VERSION     DAFNNB010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0328"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..331
                     /locus_tag="L3M60_003519"
     CDS             <1..331
                     /locus_tag="L3M60_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6228133.1"
                     /translation="EPSYFKTWITKILINTFNTKYKKTVNYLNIRDYEHKIEEVNYQQ
                     IEDKIDLYDAIDRLDEKYKTPIILQYFQDMTIGEIAEILDINENTIKTYLRRGKASLY
                     ELLKEEK"
     gene            333..1367
                     /locus_tag="L3M60_003520"
     CDS             333..1367
                     /locus_tag="L3M60_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4179 domain-containing protein"
                     /protein_id="HBN6228134.1"
                     /translation="MSKYDDIKIPESLKRVRNSAIEKAYKDKKFKRDKKRFTMTVSIA
                     IIGLGLLGIGVSNPTFAQKILRVNEMIQAIQGIFTNNELSDSITTIGESSTRDGLTIT
                     VQNAYYSQNEITLEIELKTDKPFKNTKYSKVIVKDNKDESIDDFALMTKDFNTWINGE
                     ETKGIVYESPRFKYIDEYTLKGYWQINFKSPIENHKNYDTIKMSFGLNETNYDKKSNN
                     YKYTNSINGPWILEFPVKSNMDKVKIIKPDIRQDIITVKQVALSQTSLNLKLELEKQG
                     VIEYVEVKDDNGETLGGGMESSNPNKIDLDFGLKNVNQSPKYITIFAYGNIDNGKDES
                     KAIKIKINLE"
BASE COUNT          715 a          179 c          236 g          514 t
ORIGIN      
        1 agaacctagt tattttaaaa catggataac taaaatatta ataaatacat tcaatacaaa
       61 atataaaaaa acagttaatt atttaaatat tagggattat gaacataaga ttgaagaggt
      121 aaattatcaa caaattgaag ataaaatcga tttatacgat gcaattgata ggttagatga
      181 aaaatacaaa acacctataa tacttcaata ttttcaagac atgacaattg gtgaaatcgc
      241 agagatactt gatattaatg aaaatacgat aaaaacgtac ttaaggcgag gtaaagcaag
      301 tctttatgaa ctattaaagg aggaaaagtg atatgagtaa atacgatgat ataaaaatac
      361 ctgaaagtct taagagagta agaaattcag caatagaaaa agcatataaa gataaaaagt
      421 ttaaaagaga taaaaaaaga tttactatga ctgtaagtat tgcaattatt ggattaggat
      481 tgcttggaat aggtgtatca aatccgacat ttgctcaaaa aatactgaga gtaaatgaaa
      541 tgattcaagc aattcaaggt atatttacta ataatgaact gtcagatagt attacaacta
      601 ttggagaatc aagtacaaga gatggactta ctataactgt ccaaaatgca tattatagtc
      661 aaaatgaaat taccttagaa atagaactaa aaacagataa accatttaaa aacactaaat
      721 attctaaagt catagtaaaa gacaataaag atgaaagcat agatgatttt gctcttatga
      781 ctaaggattt taatacctgg ataaatggag aggaaactaa aggcattgta tatgaatcac
      841 ctaggtttaa atatatagat gaatatactc tgaaaggata ctggcaaata aattttaaat
      901 cgccaattga aaatcataaa aattatgata ctatcaagat gtcttttggg cttaatgaaa
      961 caaattatga taaaaaatca aataattata aatatacaaa ttctataaat ggaccttgga
     1021 tattagaatt ccctgtaaaa tcaaatatgg ataaggttaa aataataaaa ccagatatta
     1081 gacaagatat cataactgtt aagcaggtag ctttaagtca aacatcatta aacttaaagt
     1141 tagaattaga aaaacaagga gttatagaat atgtagaagt taaagatgat aatggagaaa
     1201 ctttaggtgg tggaatggag agttctaacc ccaataaaat agacttagat tttggattaa
     1261 aaaatgtaaa tcaatctcca aaatatataa caatatttgc ctatggcaat attgataatg
     1321 gcaaggatga aagtaaagct ataaaaatta aaattaattt agaataatca attatccctt
     1381 aaaagttatt tagaaattta ctaattatag ataaagttaa tgcccttgaa atccaaataa
     1441 atagatttta agggcatttt tattatatat tttcttttat attactaaaa gttacatatt
     1501 tatatgtatt ttatttttat caaatctatc tataagttta tcttttcttc gctgtatcct
     1561 gtagatacaa aggcataata cactagtttc aaatatttcc cccaattttc taaatatttt
     1621 cactttttcg ttttattact atga
//
LOCUS       DAFNNB010000277         1613 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0333, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000277 DAFNNB010000000
VERSION     DAFNNB010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0333"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            393..1328
                     /locus_tag="L3M60_003521"
     CDS             393..1328
                     /locus_tag="L3M60_003521"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBN6228135.1"
                     /translation="MHPTRSFQGLILTLHNYWAQHGCVILQPYDMEVGAGTFHPATTL
                     RSLGPKPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVIMKPSPANLQELYLGSLAA
                     IGLDPLLHDVRFVEDDWESPTLGAWGLGWECWCDGMEVSQFTYFQQVCGIECAPVSGE
                     LTYGLERLAMYIQGVDSIYDLNFNGLEGDEKVTYGDVFLQAEQEYSRHNFEHANTSVL
                     LRHFEDAEAECAALLHAGAPKDGDNSPVHRMVLPAYDQCIKASHAFNLLDARGVISVT
                     ERQSYILRVRNLARQCGEAFLLTEAGGVNFKSESV"
     gene            1328..>1613
                     /locus_tag="L3M60_003522"
     CDS             1328..>1613
                     /locus_tag="L3M60_003522"
                     /inference="COORDINATES: protein motif:HMM:NF014183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBN6228136.1"
                     /translation="MPDLLLELRSEEIPARMQRKAAGDLKKLVTDGLVEAGLTYEAAG
                     DYWTPRRLTLDIRGLTARSKDVHEDRKGPSVSAPEAAIAGFLRSAGLTDIA"
BASE COUNT          322 a          496 c          479 g          316 t
ORIGIN      
        1 cgcaagctgg aagcgacttt ccaatgggcc cacgcccgcg attccaagcc tgagccggaa
       61 gatctggtca agatcgagga aaagctgaag aacggccttc ccgatgcaaa ggaaccgggc
      121 gttgccaaga atggtagcga tgccccgaaa gcaccggaac ctccagcgcc gcaaccagcg
      181 gcccccgttc cagctccgga caacaagggc tgatcatcgt tgaaaacaag ggtgctttcc
      241 cggcaccctt gccatgattt ccgctgcggg tacctgcgaa agccattgtc atggcttcct
      301 ttctcttgac cggtcagacg gcacgcctta ggacgggcac tccatttccc gtcaaaaagg
      361 cctgaccggt gaccgtccca ttgcccgatc atatgcaccc gacccgttcg ttccagggtc
      421 tcatcctgac gcttcataat tactgggccc agcatggatg tgtcatcctg cagccctatg
      481 acatggaagt cggcgcgggt acttttcacc cggcgacaac attgcgttcg ctcggtccca
      541 agccatggaa ggcagcctac gtccagccat cgcgccgccc caaggatggc cgttacggcg
      601 aaaaccccaa ccgcttgcag cactattacc agtatcaggt gatcatgaag ccatcgcctg
      661 ccaacctgca ggagctttac ctcggctcgc tggcggccat cggccttgat ccgctgctgc
      721 atgatgtgcg tttcgtcgag gatgactggg aaagcccgac gctcggcgcc tgggggctcg
      781 gctgggaatg ctggtgcgat ggcatggaag tgtcgcagtt cacctatttc cagcaggtct
      841 gcggcattga atgcgcgccc gtttcgggtg agctgaccta tggtctggag cggctcgcca
      901 tgtatatcca gggcgtcgac agcatttacg acctgaattt caatggcctc gagggcgatg
      961 aaaaggtcac ctatggcgat gtgttcctgc aggcggaaca ggaatattcg cggcacaatt
     1021 tcgaacatgc caacacgtcg gtcctgctgc ggcatttcga ggatgcggaa gccgagtgcg
     1081 cggcgcttct gcacgcgggc gcacccaagg acggcgacaa cagcccggtg caccggatgg
     1141 tccttccggc ctatgaccag tgcatcaagg cttcgcatgc gttcaacctg ctcgatgcgc
     1201 gcggtgtgat ctcggtgacg gagcggcaga gctatatcct gcgggtccgc aatctcgccc
     1261 ggcagtgcgg cgaggcgttc cttctgaccg aagccggcgg cgtcaatttc aagagtgagt
     1321 ccgtataatg cccgatcttt tgctcgaact tcgctcggaa gaaattcccg cccgcatgca
     1381 gcgcaaggcg gccggcgatc tgaaaaagct cgtcaccgat ggtctggtcg aggctggcct
     1441 cacctatgaa gcggctggtg actactggac gccgcgccgt ctcacgctgg atatccgcgg
     1501 cctgaccgca cggtcgaaag acgtgcatga ggatcgcaag ggaccgagcg tcagcgcgcc
     1561 ggaggcagcc attgcaggct ttctgcgttc cgccggcctg acggatattg cac
//
LOCUS       DAFNNB010000278         1587 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0338, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000278 DAFNNB010000000
VERSION     DAFNNB010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0338"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            16..756
                     /locus_tag="L3M60_003523"
     CDS             16..756
                     /locus_tag="L3M60_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HBN6228137.1"
                     /translation="MKNFRDLGGNKTEDGRTVKKGLFYRSAKLSNLSENDIKILKDLN
                     IKYIFDYRSDQEARKHPSTIISNIKNIRIPAMRELEESGGSFGSIEDMIDGLFEKDGA
                     FNMLNNSYYNLPINNPSYKKLVELIRDYSNLPILNHCTAGKDRTGVGSAIILMILGVS
                     RENIMKDYLKSNDFADKEIERFIDYKPKFKGIPKENLKYIFGVNEEYMKTAFRRIDEE
                     YTSVEAYLYGEFNLNKEEIKKLRNQYLE"
     gene            768..>1587
                     /locus_tag="L3M60_003524"
     CDS             768..>1587
                     /locus_tag="L3M60_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003440263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HBN6228138.1"
                     /translation="MSLNITARQVNIIKILLNSKDTISGLALSQEIGCSSKTIQNEIK
                     DINKQLRNGKILSIRGVGYKLEGSFEEINLNSNLYDDVDRVEYIIRKILTLSNEEKNT
                     IKLEDLADSMYVSVSTVKNDLKEVKRILEKYSISVKSKHKQGICVLESEDKILNCIVD
                     LSNKRDNQLSLNDFLNDDIREKKLLIKKLLLDILNENGLVLTDIEFKNVLNNILVMLS
                     RHKYNEKEFIENYVENYRRKRSSILENEQNKEIIINSIKTFCKNLKLATSIDISN"
BASE COUNT          720 a          112 c          272 g          483 t
ORIGIN      
        1 ttaagaggtg ttaagatgaa aaattttaga gatttaggtg gtaacaaaac tgaggatggt
       61 aggactgtaa agaagggatt gttttatagg tctgcaaaat tatcaaattt aagtgaaaat
      121 gatataaaga ttttaaaaga tttaaatatt aaatatatat ttgattatag aagtgaccaa
      181 gaagctcgta aacatccatc aacaataata tcaaatataa aaaatataag aataccagca
      241 atgagagagt tagaagaatc aggaggaagt tttggttcta ttgaagatat gattgatgga
      301 ttgtttgaaa aagatggtgc atttaatatg ttaaataata gctactataa tttgcctata
      361 aataatcctt catataaaaa actagtagaa cttataagag actattctaa tttaccaata
      421 ttaaatcatt gtacagctgg taaggataga actggtgtag ggagcgctat aattcttatg
      481 attttagggg tatcaagaga gaatataatg aaagattatc taaaaagtaa tgattttgca
      541 gataaagaga tagaaagatt tatagattat aaacctaaat ttaaggggat tccaaaagaa
      601 aatttaaaat atatatttgg tgtaaatgaa gagtatatga aaactgcatt tagaagaata
      661 gatgaagaat acactagtgt agaagcatat ttatatggag aatttaattt aaataaagaa
      721 gaaataaaaa aattaagaaa tcaatattta gagtaaaagg tgattttatg agtttaaata
      781 taacagcaag acaagtaaat ataataaaaa tattgttaaa ttcaaaagat acaattagtg
      841 gtcttgcctt aagtcaagag ataggatgtt cttcgaaaac tatacaaaat gaaataaagg
      901 atattaataa acaacttaga aatggtaaaa tcttgtcaat aagaggagtt ggttataaac
      961 tagaaggaag ttttgaggag ataaatctaa atagtaatct ctatgatgat gtagatagag
     1021 tagaatatat aattagaaaa atattaacct taagtaatga agaaaaaaat actataaaac
     1081 ttgaagattt agccgattct atgtatgtaa gtgtttcgac agtaaaaaat gatttaaaag
     1141 aagtaaaaag aatattggaa aagtattcaa tatcagttaa atctaagcat aaacaaggta
     1201 tatgtgtttt ggagagtgag gataagatat taaactgtat agttgactta agcaataaga
     1261 gagataatca attgagttta aatgattttt taaatgatga cataagggaa aaaaaattac
     1321 taataaaaaa attactatta gacatattaa atgaaaatgg attggtatta actgatatag
     1381 aatttaaaaa cgtgttaaat aatatattag ttatgttaag tagacataag tataatgaaa
     1441 aagaatttat agagaattat gtagaaaatt ataggcgcaa aagaagctca attttagaaa
     1501 atgaacaaaa caaagaaatt ataataaatt caataaaaac attttgtaaa aatttaaaat
     1561 tagctacatc aatagatata agtaatg
//
LOCUS       DAFNNB010000279         1580 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0339, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000279 DAFNNB010000000
VERSION     DAFNNB010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0339"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(52..147)
                     /locus_tag="L3M60_003525"
                     /pseudo
     CDS             complement(52..147)
                     /locus_tag="L3M60_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729220.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            420..>1580
                     /locus_tag="L3M60_003526"
     CDS             420..>1580
                     /locus_tag="L3M60_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBN6228139.1"
                     /translation="MNITKNFLKDYLERFGETPFLVRLKDEEEFLIGEGSPQFKVIVN
                     NDISKSELLKSTSLALGEAYINRDIEVEGDLFLTLNLLLKQIDKFNIDNSAVKHLLHS
                     SNSMRHQKKEIHAHYDIGNDFYSLWLDKTLSYSCAYFRNDNDTLYDAQVNKVHHILTK
                     LNLEEGMSLLDIGCGWGFLLIEAAKKYKIHGVGITLSEEQFKEFNARIKEENLQDYLE
                     VRLMDYRELKKSDLMFDRVVSVGMLEHVGRENYELFIKNVNAVLKDGGLFLLHYISGL
                     QESYGDPWIKKYIFPGGVIPSLREMIHICSDYKYHTIDVESLRLHYVKTLLYWQKNFS
                     ENLPKIKEMFDDKFIRMWDLYLCSCAAAFNTGIIDIHQILFTKGIKNDLPLTR"
BASE COUNT          594 a          216 c          243 g          527 t
ORIGIN      
        1 tttagctact aattcatgac taaagtcacg agtgtgcgta gctatttttt aatcaattat
       61 atattctata tcattttcat acgcgaactc taaagcttta aagctctttc ctgtgccact
      121 tgacccaacc aaagcatata cttccatcct atatctcctt ataaaatagt tttaactatt
      181 tattacaaaa ttttatttac tttatagatt aaatttatta tatcattaac agtttaatat
      241 tatatccagt accaaaattt ataggcaaaa aatatactac aacatgaaat ttctttataa
      301 aaaataaatt tcctttaaat aaaaaattta ttgaaaaaat tttacttttt taaaaaaagg
      361 actataatat attttaagca gggaaactta ctggatttac attagaagga gctggtaata
      421 tgaatattac aaaaaatttt ttaaaagact atttagagag atttggtgaa acaccatttc
      481 tagttaggct taaagatgag gaagaatttc taattggaga aggctcccct cagttcaaag
      541 ttatagtaaa taacgatatt agcaagtctg aactattaaa aagtacatct ttagcacttg
      601 gagaagccta cataaataga gatattgaag ttgagggaga tttattctta actttaaatc
      661 tacttttaaa acaaatagac aaattcaata ttgataattc agctgtaaaa catctattac
      721 actcatcaaa ttcaatgaga catcagaaaa aagaaataca tgcccactat gatattggta
      781 acgattttta tagcttatgg ctagataaga cacttagtta ttcttgtgca tacttcagaa
      841 atgataatga tactctttat gatgcacaag tcaataaagt tcaccacata ttaactaagc
      901 taaatctaga ggaaggtatg agtcttctcg atattggttg tggttgggga tttttactaa
      961 tagaagctgc aaaaaaatat aaaattcatg gtgttggaat tacacttagc gaagagcaat
     1021 ttaaggaatt taacgcaaga atcaaagaag agaaccttca agactacctt gaggtaagac
     1081 ttatggacta cagagaatta aaaaaatcag accttatgtt tgatagggtt gtaagcgttg
     1141 ggatgcttga acatgttggt agagaaaact atgaactttt cattaaaaac gtaaacgctg
     1201 ttttaaaaga tggcggatta tttttacttc actatataag tgggttgcag gaatcttatg
     1261 gagacccttg gataaaaaaa tatattttcc caggaggagt tattcctagt ctaagagaaa
     1321 tgatacatat ttgtagtgat tacaagtatc atactataga tgtggaaagt ctaagattac
     1381 attatgtaaa aactttacta tactggcaaa aaaacttctc tgagaatctt ccaaagatta
     1441 aagaaatgtt tgatgataag tttataagaa tgtgggattt atacctttgt tcttgtgccg
     1501 ctgctttcaa tactggtatt atagatatac atcaaatact tttcactaaa ggcataaaaa
     1561 atgatttacc tctaactaga
//
LOCUS       DAFNNB010000280         1518 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0352, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000280 DAFNNB010000000
VERSION     DAFNNB010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0352"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            96..449
                     /locus_tag="L3M60_003527"
     CDS             96..449
                     /locus_tag="L3M60_003527"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228140.1"
                     /translation="MQGILYEEPSFWLFVLVTCVMGGWAAWMAGRACAKTWRSHTILI
                     FYMLLLGIGVRFIHFALFEGTMVTLHYYVVDTIVLIIIGTLGYRYTRTNQMVQQYHWL
                     YEKVSPFSFKERKGA"
     gene            577..>1518
                     /locus_tag="L3M60_003528"
     CDS             577..>1518
                     /locus_tag="L3M60_003528"
                     /inference="COORDINATES: protein motif:HMM:NF024850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HBN6228141.1"
                     /translation="MKKSLFSGVAVAAMLALGGHAYADLLVGIGAPLTGPNAAYGAQI
                     QKGAEAAAEVVNAAGGVNGEKIKFVLGDDVSDAKQGVSVANKFVADGVKFVVGHFNSG
                     VSIPASEVYAENGIVEVTPAATNPVFTERGLWNTFRTCGRDDQQGAIAGAYIAEHYKD
                     AKIAVIHDKTPYGQGLADETKKTIEAKGLKPVLYEGVNVGDKDFSALIAKAKQAGVTV
                     LYWGGLHTEAGLIIRQLADQGLKVKFISGDGIVTNELAAIAGDAVVGTLNTFGPDPRN
                     NPANKELVEKFRKAGFEPEAYTLYAYAAVQAIVEAATA"
BASE COUNT          339 a          412 c          435 g          332 t
ORIGIN      
        1 cgcgcggcct atctcgaggg cgggcgtcac tgacaaaaat aggaccggct ttccagcggg
       61 tctggaaaat ccggtgctgc aaggggaata ctgccatgca aggcattctg tacgaagaac
      121 cctcattctg gctgtttgtg ctggtaacct gcgtcatggg tggctgggcc gcatggatgg
      181 cgggccgcgc ctgcgccaag acatggcgtt cccatacaat cctgatcttc tatatgctgc
      241 ttctcggcat cggcgtccgc ttcatccact tcgccctgtt tgagggcacg atggtgacgc
      301 tgcactacta cgtcgtcgac acgatcgtgc tgatcatcat cggtacattg ggctatcgct
      361 acacccggac aaaccagatg gttcagcaat atcactggct ctatgaaaaa gtttccccct
      421 tctcgttcaa ggaaagaaaa ggggcctaag caatctcgct gacgcgaaat gaatcagcgt
      481 gacaaccgcc ggaaaatcgg caaatgtggt cttcaagcga tgggaagatt tcatcgttag
      541 gggtttcgta agtacctaga aaatgggagt tttcaaatga agaagtcact gttctcgggt
      601 gttgctgttg cagcaatgct cgctttgggc ggtcacgcct atgctgatct tctggtcggt
      661 attggtgctc cgttgaccgg ccccaatgcc gcttacggcg cgcagatcca gaagggtgcc
      721 gaagcggccg cagaggtggt caatgcggct ggcggcgtga atggcgaaaa gatcaaattc
      781 gttctgggcg atgacgtttc cgacgccaag cagggcgttt cggttgccaa caagttcgtt
      841 gccgatggcg tgaagttcgt tgtcggtcac ttcaattccg gcgtatccat tccggcatcc
      901 gaagtctatg cggaaaacgg cattgttgaa gtaacaccag ccgcgacaaa ccctgtgttc
      961 actgaacgcg gcctgtggaa cacattccgt acatgcggtc gcgatgacca gcagggtgcc
     1021 attgccggtg cctatatcgc cgagcattac aaggatgcaa agatcgcggt catccatgac
     1081 aagacgcctt atggtcaggg tcttgccgat gaaaccaaga agacgatcga agcaaagggc
     1141 ttgaagcctg ttctttatga aggcgtcaac gttggcgaca aggatttctc cgcactgatc
     1201 gccaaggcaa agcaggccgg cgttaccgtc ctttattggg gtggtctgca taccgaagct
     1261 ggtctgatca tccgccagct ggctgatcag ggcctgaagg tcaagttcat ctccggcgac
     1321 ggcatcgtca ccaacgagct cgcagcgatt gccggcgacg cggtggtcgg tacactcaac
     1381 acattcggtc ccgatccgcg caacaacccg gccaacaagg aactggtcga gaagttccgc
     1441 aaggccggtt ttgagccgga agcctacacc ctctacgcct atgcagccgt gcaggccatc
     1501 gtcgaagcag caaccgct
//
LOCUS       DAFNNB010000281         1488 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0359, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000281 DAFNNB010000000
VERSION     DAFNNB010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0359"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            166..840
                     /locus_tag="L3M60_003529"
     CDS             166..840
                     /locus_tag="L3M60_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228142.1"
                     /translation="MMLKYITKHKNMEESRMKKVLLLITVCLLSVGLVACSSTNNKDT
                     GNTTKKEKKANENEVDGIKITIKNVTKEDIKGDINEDGSFNENGEYFSDGIEKKKAAD
                     YVYEVVNVEVENKTDKAVKLFQTGWNAVGTDGYEFKDIKVTDKLDNQQVPANYKFDAQ
                     VKILVEKNMNVKEIVLKYNLKDYSRLFEAMEYANQGASKSDVEKKFPELYKDNWIELG
                     EIKVEQ"
     gene            complement(868..1167)
                     /locus_tag="L3M60_003530"
     CDS             complement(868..1167)
                     /locus_tag="L3M60_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228143.1"
                     /translation="MDTLLKYHRKKKKLTQRRLSELTGISQSYISRMENKKFKHSPTL
                     TQIILLADILEIDQLILSEFFLEKEREHLKNTKKYNKKAIKTLSRDFLKEYKNKD"
BASE COUNT          620 a          151 c          239 g          478 t
ORIGIN      
        1 aatatcattt agaagcgata caagacacca gaaacaattt ttctgtttgc tataaatagt
       61 ttatgttaaa tagcataaaa atattcttaa aatggaaata aataggtatt gctcttttga
      121 atcattttta tactactaga atagaaaatt aaaggaatat aagttttgat gttgaaatat
      181 ataacaaaac ataaaaacat ggaggaatcg aggatgaaaa aagtattatt attaattaca
      241 gtatgtttat taagtgtagg gttggttgct tgttcatcaa ctaataacaa agatactgga
      301 aacacaacta aaaaagaaaa gaaagcaaat gaaaatgagg ttgatggaat taaaataact
      361 ataaaaaatg ttacaaaaga agatataaaa ggagatataa acgaagacgg aagttttaac
      421 gaaaatggag aatattttag tgatggaatc gaaaagaaaa aggcagctga ttatgtgtac
      481 gaagttgtaa atgtagaagt agaaaataaa acagataaag cggttaaatt atttcaaaca
      541 ggatggaacg cagtcggtac agatggttac gaatttaaag acataaaagt aactgataaa
      601 ttagacaatc aacaagtacc agctaactac aaatttgatg cacaagttaa aatattagtt
      661 gaaaagaata tgaatgtaaa agaaatagtt ttaaaatata acctgaaaga ttattcaaga
      721 cttttcgagg caatggagta cgcaaatcaa ggagcatcaa aatcagatgt agaaaagaaa
      781 tttccggaat tatataaaga taactggatt gagctaggag aaattaaagt agagcagtaa
      841 aaaaaggggc taggtataaa taattaccta gtccttattt ttatattctt ttaaaaaatc
      901 cctgctaagt gttttaattg cttttttatt atatttttta gtatttttta aatgctccct
      961 ttctttttcc aaaaaaaatt cagataaaat caattggtca atttctaata tatcagctaa
     1021 taaaattatt tgagttaagg ttgggctgtg tttaaatttt ttgttttcca tccttgaaat
     1081 gtaactttga cttattcctg tcaattcact taatcgacgt tgagtgagtt ttttcttttt
     1141 tctatgatat tttaataatg tatccatagt agaaattaaa ctataatgtt aaaaaacatt
     1201 aaaatcaatg ggaaaaaatt acatttcaca gaacgataaa attaattggt aaaaattgta
     1261 aaatgacgtc ggaatattcg tcgtacgaat attttttaca tacgttttta tgctactatt
     1321 gtagtaaaga tatgaagcgc atcaaatctt taggaaaaca atttcaagca ctattagtat
     1381 taaaaacaca caacaaataa aaagttttga gtgtaaattt tttagaaaaa ctaatcagaa
     1441 tatttaatga ttttggaggt tttggattta tggaagaaaa aaatacaa
//
LOCUS       DAFNNB010000282         1464 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0365, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000282 DAFNNB010000000
VERSION     DAFNNB010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1464
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0365"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..1256
                     /locus_tag="L3M60_003531"
     CDS             <1..1256
                     /locus_tag="L3M60_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding protein Cwp5"
                     /protein_id="HBN6228144.1"
                     /translation="GGTAVLNKDIENKLKGNGLNVERINGKDRYETSLILANKLKDIK
                     DIKEVAVVNGEKGLSDAVSVGAPAAQNKMPIILSSPKDGVEAFDKFIRDEKVIKAYVI
                     GGTNSVSRAVEKSLPNAERLSGKDRNETNAKVIEKFYSDTSLSNLYVTKDGSKNENQL
                     IDSLAVGVLAAKNESPVVLVGNKLNTKQRDILSTKKLSTITQVGGNGNEEAFDEIKSL
                     QEKTVFEAKTVEELTDMINIASPNDIINFKPKEDTVNEAFRMVTNKPITVNIKGDCSK
                     TLTVDMPNGEVNNYATLVNVIVRNIGEGGFNNHDTITILSVRDKNGRVIENTRNSDID
                     TLMILASANDTKLINDGYIGKLIDNSSNSDITNNGTIDKKVNQVEDLEAKVDSIEKAI
                     DSISQKINKIQDILDKLGFLKKFLS"
BASE COUNT          612 a          134 c          289 g          429 t
ORIGIN      
        1 taggaggtac tgctgtactt aataaagata tagagaacaa attaaaaggt aatggactta
       61 atgtagaaag aattaatggt aaggatagat atgaaacttc actaattttg gctaataagc
      121 ttaaggatat aaaagatatt aaagaagttg ctgtagtaaa tggtgaaaaa ggactgagtg
      181 atgctgttag tgttggagca ccagcagctc aaaataagat gcctataatt ttgtctagtc
      241 ctaaggatgg agtagaagca tttgataaat ttataagaga tgagaaagtt ataaaagcat
      301 atgtaattgg agggacaaat tcagtttcaa gagctgtaga aaagagtctt cctaatgcag
      361 aaagattaag tggaaaagat agaaatgaga ctaatgctaa agttatagaa aagttttatt
      421 ctgatacaag tttaagtaat ctatatgtaa ctaaggatgg aagcaaaaat gaaaatcaac
      481 tgatagactc attagcagtt ggagtgctgg ctgcaaaaaa tgaatctcct gttgttttgg
      541 ttggcaataa attaaacaca aaacaaaggg atatacttag tactaaaaaa ttaagtacta
      601 taacacaggt aggtggaaat ggaaatgaag aagcttttga tgagattaaa agtttacaag
      661 aaaaaactgt gtttgaagca aaaactgttg aggaattaac tgatatgata aatatagcaa
      721 gtcctaatga cattataaat tttaaaccaa aagaagatac tgtaaatgaa gcttttagaa
      781 tggtgacaaa taaaccaata actgtgaata taaaaggaga ttgttcaaaa actcttacag
      841 tagatatgcc taatggtgaa gtgaataact atgctacttt ggtaaatgtt atagttagaa
      901 atattggaga aggtggattt aataatcatg atacaatcac catattgtca gttcgtgaca
      961 aaaatggtag agttatagaa aatactagaa attcagatat agatacgttg atgatattag
     1021 cttctgctaa tgatacaaaa ttgattaatg atggatatat aggaaagctt atagataatt
     1081 catctaatag tgatataact aataatggta ctatagataa aaaagttaat caggttgagg
     1141 atttagaggc taaggttgat tcaattgaaa aagctataga ttctataagt cagaaaatca
     1201 ataaaataca agatatacta gataagcttg gatttctaaa gaaatttctt agctaaaaat
     1261 atgtatattt aagtgaactg tgtgttggaa ttatatctga agatatatag ttaaatgggt
     1321 ttatagataa ataggcttat agctaaatat atagttcaat atatagttaa ataaatcttg
     1381 gggaggtaac atggggagcg gaattgaatg taacaaaaga atacataaaa gtattgacaa
     1441 aactagaata aagaaaaata atct
//
LOCUS       DAFNNB010000283         1430 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0374, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000283 DAFNNB010000000
VERSION     DAFNNB010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0374"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            256..594
                     /locus_tag="L3M60_003532"
     CDS             256..594
                     /locus_tag="L3M60_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003423377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228145.1"
                     /translation="MANIGKIIGDRIKVYRTKLGYSQEFLAEKAGLHPTYIGQIERAE
                     TNTSINIIMKIATALDIPLELLFANIITTEGVDNSVPLECYEMINKLTEKEQIAMLEL
                     IKCIIKYKEI"
     gene            616..933
                     /locus_tag="L3M60_003533"
     CDS             616..933
                     /locus_tag="L3M60_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228146.1"
                     /translation="MNYIGKRLKEERKKANLTSKEFANMVGVSPWYITQIESGKKNPS
                     LKTFIKFVSILNISADVLIKDITSTGKTYLENDINEELKDLNSRELNLILQIIDIIKK
                     NKI"
     gene            1083..>1430
                     /locus_tag="L3M60_003534"
     CDS             1083..>1430
                     /locus_tag="L3M60_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228147.1"
                     /translation="MIVIEGSDKFKIAKEYIDVEYTLFSKVTFKYEKLKFKDNAELEK
                     IKMFKYKNGYIPNKLNLSLGYGFSSYKKQIIRETVDTLRLTEIFSSENIEDIKFIKDG
                     TKKLEISIEKVVKF"
BASE COUNT          626 a          140 c          206 g          458 t
ORIGIN      
        1 ataaaaaatg ctataattaa catattatca attcgtttta ttattgtagt acggctatga
       61 aattataaaa atatttgtct aattaaaaat atgatttatc catatttgtt tattgtagat
      121 acagactata caaaaataaa tagcacttat ttcagtataa aaacatatat agtctatatt
      181 taaactaaaa aagtataaat agtctatata aaacaactta aactatgaat ataatctaag
      241 gtggagggat aataattggc aaatatagga aaaataatag gagatagaat aaaagtctat
      301 agaacaaaat tgggatatag tcaagagttt ctagcagaaa aggcaggttt acatccaaca
      361 tatattggtc agattgaaag agctgagaca aatacatcta tcaatattat aatgaaaatt
      421 gctactgctt tagacatacc acttgaattg ttatttgcaa atattattac aacagaaggc
      481 gttgataact ctgtaccttt agaatgttat gaaatgatta ataaactaac tgaaaaagaa
      541 caaattgcaa tgctagagtt aataaagtgc atcattaaat acaaagaaat ataagcgagg
      601 gaaatagtaa caataatgaa ttatataggt aaaagactta aagaagaacg gaaaaaagca
      661 aatttaactt caaaagaatt tgcaaatatg gttggagtat cgccttggta tataactcaa
      721 atcgaaagtg gtaaaaagaa tccaagtctt aaaacattca taaaatttgt aagtatatta
      781 aatatctctg cagatgttct tataaaagat attacttcca ctggaaagac atatttagaa
      841 aatgatataa atgaagagtt aaaagactta aattcaagag aactaaatct gatattacag
      901 attatagaca tcataaaaaa gaataaaata taattcttta tattttatat aaaaaaaaag
      961 gttatttgaa ataaataacc tttttttgtc tttttgtgtt aacattgtct tatagtgcta
     1021 atgagtacta ttagaccatg ttctatggcc ttattttaat ataaaacgat tggagatagg
     1081 ctatgattgt gattgaaggt agcgataaat ttaaaattgc aaaagaatat attgatgtag
     1141 aatatactct ttttagcaaa gtaactttta agtatgaaaa gttaaaattt aaagataatg
     1201 ctgaattaga aaaaattaaa atgtttaaat ataaaaatgg ctacatccct aataaattaa
     1261 acctttcttt aggatatgga ttctcttctt ataaaaaaca aataattaga gaaactgtag
     1321 atactttaag attgacagaa attttttcaa gcgagaacat agaagatatt aaatttataa
     1381 aagatggtac aaaaaaatta gaaattagca tagagaaagt tgtgaaattt
//
LOCUS       DAFNNB010000284         1411 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0385, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000284 DAFNNB010000000
VERSION     DAFNNB010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0385"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            56..1165
                     /gene="ugpC"
                     /locus_tag="L3M60_003535"
     CDS             56..1165
                     /gene="ugpC"
                     /locus_tag="L3M60_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003242648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HBN6228148.1"
                     /translation="MSEVILKNISKLYSNGFNAVKNINIDIKDKEFIVLVGPSGCGKS
                     TTLRMIAGLEEISEGELYIGDKLVNDIEPKDRDIAMVFQNYALYPHLSVYDNMAFALK
                     LRKLPKDEIDKKVKEAAKILDLLPLLNKKPKTLSGGQRQRVALGRAIVRNPKVFLMDE
                     PLSNLDAKLRTAMRTEITKLHQQLGTTFIYVTHDQVEAMTMADRIVVMKDGVVQQIAT
                     PQDVYDYPANIFVAGFIGAPQMNFIDVTLIEENDEIYAQNEHIKLKLNKEKHYDLIKD
                     NYINNEVVIGIRPEDIHVEDTFIKSNPDTCFKSRIEIKELMGAETYAHLKLGENTITI
                     RFDSKNRINVGDDLTLSVDNSRIHIFDKETTLAIR"
     gene            1195..>1411
                     /locus_tag="L3M60_003536"
     CDS             1195..>1411
                     /locus_tag="L3M60_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HBN6228149.1"
                     /translation="MEELKDFLERQLNKKINIYPYENQKLDASSHLVTFNNSIYVIDF
                     LDKNNLDNLKGNFYLIYQPHIEFEYLKN"
BASE COUNT          583 a          178 c          215 g          435 t
ORIGIN      
        1 ataataaaaa aatttgatac tatataatta aaatttaaat atcaaggaga atattatgtc
       61 agaagtaatt ttaaaaaata tatcaaaact ttactcaaat ggatttaatg cagtaaaaaa
      121 tataaacata gacattaaag ataaagaatt tatcgtttta gtaggaccat ctggttgtgg
      181 taaatcgact acacttagaa tgattgctgg tcttgaagaa atctctgaag gagaactata
      241 cattggagat aaacttgtca atgatataga accaaaagat agagatatag ctatggtttt
      301 tcaaaactat gctctatatc ctcacttatc cgtctatgat aacatggcat ttgcacttaa
      361 acttagaaaa ttgcctaaag atgaaataga caaaaaagtt aaagaggctg ctaaaatatt
      421 agatttacta cctcttttaa ataaaaaacc taaaacattg tctggaggac aaagacaaag
      481 ggttgcttta ggacgtgcaa tagttagaaa tcctaaggta ttcttaatgg atgaacctct
      541 atccaactta gatgcaaagc ttagaactgc tatgagaaca gagataacta agctacacca
      601 acagcttgga actacattta tctatgtaac acacgaccaa gttgaagcta tgactatggc
      661 tgatagaatc gtagtaatga aagatggagt ggttcaacag attgcaactc cacaagatgt
      721 atacgattat ccagctaata tatttgttgc aggatttatt ggagcaccac agatgaattt
      781 tattgatgta acattaatag aagaaaatga tgaaatttat gcacaaaatg aacatattaa
      841 gcttaaatta aataaagaaa aacactatga tttaataaaa gacaattata ttaataatga
      901 agttgttata ggtatcagac cagaagatat tcatgtagaa gatactttta ttaaatctaa
      961 tccagatact tgttttaaat caagaattga aataaaagaa cttatgggag cagaaactta
     1021 tgctcactta aagctaggcg aaaatactat aacaattaga tttgacagta aaaacagaat
     1081 taatgttggt gatgacttaa cactttcagt tgataacagt agaatacaca tatttgacaa
     1141 agaaactact ttggctataa gataagaata taatcttatt tttaaggaga ctttatggaa
     1201 gagttaaagg attttttaga aagacaactt aataaaaaaa taaatatcta tccatatgaa
     1261 aaccaaaagt tagatgctag tagccattta gttactttca ataattctat ttatgttatt
     1321 gattttctag ataaaaataa tcttgataat ttaaaaggca acttttattt aatttaccaa
     1381 cctcatatag aatttgaata tctaaaaaat a
//
LOCUS       DAFNNB010000285         1393 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0395, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000285 DAFNNB010000000
VERSION     DAFNNB010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0395"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..182)
                     /locus_tag="L3M60_003537"
     CDS             complement(<1..182)
                     /locus_tag="L3M60_003537"
                     /inference="COORDINATES: protein motif:HMM:NF013862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HBN6228150.1"
                     /translation="MNHKPKVINIALQGGGSHGAFTWGVLDRILEDGRLTIEGISGTS
                     AGAMNAVALADGWSRNG"
     gene            complement(182..961)
                     /locus_tag="L3M60_003538"
     CDS             complement(182..961)
                     /locus_tag="L3M60_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003113501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HBN6228151.1"
                     /translation="MAKKTAVVTGSNSGIGLGSAHALAAAGYNVVINSFTDRPEDHDL
                     AKQIAAQHSTGVAYIKADMSKGDECRALIDQAAAAFGSVDVLVNNAGIQFVAPVDEFP
                     TEKWDAIIAINLTSAFHTTAAALPYMKKAGWGRIINIASAHGLRASPFKSAYVAAKHG
                     ILGLTKTVALEVAEKHITSNAICPGYVLTPLVEAQIPDQMKAHNMDRETVIREVMLDR
                     QATKEFATVDQIGATVVFLASDAAAQITGTSISVDGGWTAQ"
     gene            1178..>1393
                     /locus_tag="L3M60_003539"
     CDS             1178..>1393
                     /locus_tag="L3M60_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBN6228152.1"
                     /translation="MSDYYSQPFARRASVGVDVGGVMVGGGAPVVVQSMTNTDTADVD
                     GTVAQVAALSRAGSQIVRITVDRDESAA"
BASE COUNT          251 a          387 c          443 g          312 t
ORIGIN      
        1 ccgttgcggc tccagccatc agccagcgcg acagcattca tggcgccggc gctggtgccg
       61 gatatgccct cgatggtcag ccggccatct tccagaatgc ggtcgagaac accccaggtg
      121 aaggcgccgt gcgagccgcc gccctgcagc gcgatgttga tgactttggg tttgtggttc
      181 atcactgcgc cgtccagccg ccatcgacgg agatggatgt accggtgatc tgcgccgctg
      241 catcgcttgc gagaaacacc accgttgcgc cgatctggtc gaccgtggcg aattccttgg
      301 tcgcctgcct gtcgagcatg acttcgcgga tgacggtttc gcggtccatg ttgtgcgcct
      361 tcatctggtc ggggatctga gcctcgacga gcggtgtcag gacataaccg gggcagatgg
      421 cgttgctggt gatatgcttt tcggccactt ccagcgcgac ggttttggtc agtccgagaa
      481 tgccgtgctt ggccgccaca taagccgact tgaacggcga tgcgcgcagg ccatgcgccg
      541 aggcgatatt gatgatgcgg ccccatccgg ccttcttcat atagggaagc gcggcagcgg
      601 tggtgtggaa cgccgaggtc aggttgatgg cgatgatcgc atcccatttt tccgtcggga
      661 attcatcgac aggggcgaca aactggatgc cggcattgtt gaccagcaca tcgacgctgc
      721 cgaatgcggc agcagcctgg tcgatcaggg cacggcattc gtcacccttg gacatgtcgg
      781 ccttgatata ggcgacgccg gtggaatgct gtgcggcgat ctgcttggca agatcgtggt
      841 cttccgggcg gtccgtgaag gaattgatca ccacattgta acccgccgct gcgagggcgt
      901 gggcggaacc cagtccgatg ccggagttgg aacctgtgac gactgcggtc ttcttggcca
      961 tgagaaactt cctttggttt aacaaccgtg ttgaaggcgt ctggaatata ttgcattgca
     1021 gcaaaaatac cagctttgga acgtgaacac tttgtcatac ggttcactgt ggcgtgatcg
     1081 ggaagcgcat ttgctttgaa tttgccgcgt tcatgtcaca tggtgttgac acaaggcgtt
     1141 ccctgcgcca cgtgaaggga atatctggag tttgacaatg tctgactatt attcgcagcc
     1201 ctttgcccgc cgcgcctccg ttggcgtcga tgtgggcggg gtcatggttg gcggaggcgc
     1261 accggtcgtc gtgcagtcga tgaccaatac ggatacagcc gatgtggacg gcaccgttgc
     1321 acaggtggca gccctttccc gcgcgggatc gcagattgtc cgcatcaccg ttgaccggga
     1381 tgaatccgct gcc
//
LOCUS       DAFNNB010000286         1328 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0417, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000286 DAFNNB010000000
VERSION     DAFNNB010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1328
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0417"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(27..1103)
                     /locus_tag="L3M60_003540"
     CDS             complement(27..1103)
                     /locus_tag="L3M60_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HBN6228153.1"
                     /translation="MSKKLFIPGPIDVKEEVLQKMATPMIGHRGKDASVLQKSISEKM
                     QKLFYTNNTILLSTSSGTGLMEGSIRSCTSKKAAVFSCGSFGDRWYKMAVANNVPADI
                     FKVELGEATTPEMVDKVLSTGEYDLITVTHNETSTGIRNPIEEIGEVVKKYKDVIYCV
                     DTVSSAGGIKVEVDKIGIDICITSVQKALGLPPGMSICTFSQKAIDRAKQVPFRGVYL
                     DLLAMYEYLIKKNYQYPSTPSLSHMFALDFQLDNILDEGLDNRFNRHEDMANLVRNWA
                     KKHFQIFTNENHLSNTLTVIENTQGISVSNLNSKLQERGFQIANGYGDLKEKTFRISH
                     MGDYTVEDVQELLDNIDDILGFNK"
BASE COUNT          397 a          227 c          179 g          525 t
ORIGIN      
        1 cttaaaatat acatattttt acaatattat ttattaaaac ctaatatatc atcaatatta
       61 tctaataatt cttgtacatc ttcgacagta tagtcaccca tgtgtgaaat tctaaaagtt
      121 ttttctttta aatctccata gccatttgct atttggaatc ctcgttcttg taattttgaa
      181 tttaaatttg atacactaat accttgagta ttttctataa cagtaagtgt attggataag
      241 tgattttcat tggtaaaaat ttggaagtgt tttttagccc agtttctaac taagtttgcc
      301 atgtcttcat gtctgttgaa tctgttatct aggccttcat ctaatatatt atctaattgg
      361 aaatctaaag cgaacatgtg agaaagagat ggtgttgaag ggtattggta gtttttcttg
      421 attaagtatt catacattgc taacaaatca agatatactc ctctaaaagg tacttgctta
      481 gctctatcga ttgctttttg agaaaatgtg caaatagaca ttcctggtgg taatcccagt
      541 gctttttgta ctgatgttat acaaatatct attccaatct tatcaacctc gacttttatt
      601 ccaccggctg aacttacagt atctacacag tagattacat ctttatactt tttaacaact
      661 tctccaattt cttctattgg atttcttatt ccagttgaag tttcattgtg agttacagtt
      721 attaaatcgt attctccggt agaaagcacc ttatcaacca tttctggcgt tgttgcctct
      781 cctaattcaa ctttaaatat atccgctggg acattattag ccactgccat tttgtaccat
      841 ctatcaccaa aggatccaca ggaaaatact gctgcctttt tggatgtaca ggaacgaatg
      901 gagccttcca ttaaaccagt tccggatgac gtggataaaa gtatcgtgtt gttcgtatag
      961 aatagttttt gcattttttc acttatactc ttttggagta cagatgcatc ttttcctcta
     1021 tgtcctatca ttggtgtagc catcttttgc aagacttctt ctttaacatc aataggacca
     1081 ggaataaata atttcttact cattataatt cccccctttt tagtttgtaa tatggatagc
     1141 gtttttactg tccttatttt taataactat aatatcaagc tgaattaact atgtcaacat
     1201 aaaatttatt ttttatatat aatttatttt atatatttta tgaaaacata tatttcgcac
     1261 cttttcataa gtctgaatat tctgaattaa ttcctcaaaa aaaatatact ttttatctat
     1321 ataaatta
//
LOCUS       DAFNNB010000287         1325 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0421, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000287 DAFNNB010000000
VERSION     DAFNNB010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0421"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(99..341)
                     /locus_tag="L3M60_003541"
     CDS             complement(99..341)
                     /locus_tag="L3M60_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228154.1"
                     /translation="MNRIAYQTLTVSNSLSFLRPATPVSILKSSGVATHTLISTDKHS
                     NGYNFSYAAHSDEGVRTNLLSKLKGKKISYYKVYWE"
     gene            482..1024
                     /locus_tag="L3M60_003542"
                     /pseudo
     CDS             482..1024
                     /locus_tag="L3M60_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728737.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
     gene            1050..1238
                     /locus_tag="L3M60_003543"
     CDS             1050..1238
                     /locus_tag="L3M60_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009888246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228155.1"
                     /translation="MNNKKKAFLISGAINVILLIIVFTLFRYNIIENSTKNMIITVIL
                     IFIFEIIKIKILEKYYNL"
BASE COUNT          508 a          140 c          194 g          483 t
ORIGIN      
        1 taaaatttag tagttgtttc tgctaaattt ttattgattt tttgaaatag atatttattc
       61 ttttcaattt tcatttgatt aaagtcataa acttttcttt attcccaata aactttgtaa
      121 taagaaattt tctttccttt aagtttactt aacaagtttg ttcttacgcc ttcatctgaa
      181 tgtgcagcgt atgaaaaatt atatccattt gaatgtttat ctgtagatat tagtgtatga
      241 gttgctactc cactactttt taatatactt acaggtgttg ctggtcttaa aaaacttaaa
      301 gagtttgata cagtaagtgt ttgatatgca attcgattca cccaatgtat tttaaactct
      361 cttgctccag cccaagaagg tgatctatta ctagtagaat aataatacca tggattatta
      421 ttagtccaga atgttactcc agcctcatat aagcattgtg aaacaaagtt agcataatct
      481 ccatatccat ccttttagtg atggtaatgg aagaacatca agagcattat catatctata
      541 tctgattgat aaaggatatg atacttttaa agagttttca atatcatata tgatatctaa
      601 aaacagaact aagtattata aggctatact tgatgtagag aataagggat ataatttaac
      661 tgtatttata gagttcatgc taaagtccat aatgcaatct ataaatgaaa tgagagacat
      721 gcatgatagg aattctttag agagtgtgtt gaaagaagag ttatttgaaa atgacattac
      781 tctatcagca acagaagaac atatattaaa atatatctgt agtaaagata actattctat
      841 gacattggaa aactatatta aaaggaataa aagtagatac ttaaaggctg gaataaaaga
      901 aatagactta attgaccagt tgatggaagt gtttaataat cttgaagaaa tagagctttt
      961 atcaagggaa aaggatgtat ataaagttaa tgagaagtac ttaaaaatgc ttgatataga
     1021 ctaatttaga taatctagag agggaatata tgaataataa gaaaaaggca ttcttaattt
     1081 ctggtgctat aaatgtaatt ttattaataa ttgtttttac gttatttcgt tataatatta
     1141 tagaaaatag tacaaaaaat atgataataa ctgtgatctt aatttttatt tttgaaatta
     1201 taaaaataaa aattttagaa aagtattaca atctataaaa aatatatttt aaataatgta
     1261 taatatacat ttatcgtttg aataaaagat tttataagct ttttaagaaa ctgtaagtaa
     1321 attta
//
LOCUS       DAFNNB010000288         1256 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0451, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000288 DAFNNB010000000
VERSION     DAFNNB010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0451"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            100..975
                     /locus_tag="L3M60_003544"
     CDS             100..975
                     /locus_tag="L3M60_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine DNA glycosylase"
                     /protein_id="HBN6228156.1"
                     /translation="MNVYEKGNGVILEGVTDFDPVHIFECGQCFRWHKQEDGSYTGVA
                     KGRILNVKKENDKIYLNNTNLKEFNSIWYNYFDLGTDYTEIKNKLKNMDEYLNKATEF
                     GWGIRILRQDGWEMLISFIISSNNRIPMIQRAIENLSRKFGKYIGEYEGKEYYAFPTP
                     EELNKASQEEIRACQTGFRDKYIKSTTQAVIENNDKVSEYTNLSTEDCRKELLKFNGV
                     GPKVCDCIALFGMQKYDSFPVDVWVKRVMQEFYIDEDMSLPKMRTYGIDKFKEMSGFA
                     QQYLFYYARELGIGK"
     gene            993..>1256
                     /locus_tag="L3M60_003545"
     CDS             993..>1256
                     /locus_tag="L3M60_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HBN6228157.1"
                     /translation="MGVIGTIFLFYLIISYLAGAIISVIILLENRDPAKTMSWLLMFI
                     IFPGIGLMIYAISGRNIRKRKLFKTQKLANNIKEKKLFDTLEKI"
BASE COUNT          520 a          117 c          231 g          388 t
ORIGIN      
        1 acttatcttt gtgttaaaat aagtaaaatg cacatattaa agctaagttt ttaagtaaat
       61 aaacaaaatg aaaatataaa tattaaggag atttagtata tgaatgttta tgaaaaaggt
      121 aatggagtta tactagaagg agtaacagat tttgacccag tacatatatt tgagtgtggt
      181 caatgtttta gatggcacaa acaagaagat ggctcataca ctggtgttgc aaagggaaga
      241 atattaaatg tcaaaaaaga gaatgataaa atatatttaa ataatacgaa tttaaaggag
      301 tttaatagta tctggtataa ttactttgat ttaggaactg attatacaga aataaagaat
      361 aagctaaaaa atatggatga atatttaaat aaggctactg aatttggatg gggaataaga
      421 atattaagac aagatggatg ggaaatgctc atatcattta taatttcctc taataatagg
      481 attcctatga tacaaagagc tatagaaaat ttatcaagaa aatttggtaa gtatataggt
      541 gaatatgaag gtaaagaata ctatgcattc ccaacaccag aagagttaaa taaggcctca
      601 caagaggaaa taagagcatg tcaaacaggt tttagagata agtacataaa aagtactaca
      661 caggcagtta tagagaataa tgataaggta tccgaatata caaatttaag tacagaagat
      721 tgtagaaaag agcttttaaa atttaatgga gtaggcccaa aagtatgtga ctgtattgca
      781 ttatttggta tgcaaaaata tgattctttt ccagttgatg tttgggttaa gagagttatg
      841 caagaatttt atatagatga agacatgagt ttacctaaaa tgagaactta tggaatcgat
      901 aaatttaaag aaatgtctgg atttgctcaa cagtacttat tttactatgc aagagaactc
      961 ggtataggaa aataaattaa gggggagaaa ttatgggagt aataggaaca atatttctgt
     1021 tctatttaat tatatcatat ctggctggag ccataatttc tgtgattata ttacttgaaa
     1081 atagagatcc tgcaaaaaca atgtcttggc tattaatgtt tattatattt ccaggtatag
     1141 gtttaatgat atatgctatt tcaggtagaa atattagaaa aagaaaatta tttaaaactc
     1201 aaaaactagc taataatata aaagaaaaga aactttttga tacattagaa aagata
//
LOCUS       DAFNNB010000289         1222 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0472, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000289 DAFNNB010000000
VERSION     DAFNNB010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0472"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            246..>1222
                     /locus_tag="L3M60_003546"
     CDS             246..>1222
                     /locus_tag="L3M60_003546"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HBN6228158.1"
                     /translation="MTDVIDRETETNSAQPGISDARLKELVEQARAEGLQLTGDGGLL
                     SKLTKLVVESALEGEMDDHLGYGKSDPAGRGSGNSRNGRRAKNVLTEAGPVELEVPRD
                     RDSSFEPKIVAKRQRRLSGVEDMVISLSAKGLTTGEIAAHLAEVYGADVSKQTISTIT
                     ERVMEGMAEWQNRPLDPVYPVLFIDCIHVKIREGNVANRPIYVAMGVTVDGTRDILGL
                     WVGEHGDGEGAKYWLRVLSELKNRGVQDVLIVVCDGLKHLPDSIEQVWPQTIVQTCVV
                     HLLRNTYAYASRKDWSDIAKDLKPVYTAPSEQAALDRFAEFSDKWEKRYP"
BASE COUNT          275 a          362 c          379 g          206 t
ORIGIN      
        1 aacctcaacc acccgaccat cacgaacagc cgccgacacc aaagacccac ccaacgtcgg
       61 ctcatccaac gtcggcaaca tctccaccgt gaacggctca cccgaatcaa tcagacggtc
      121 aacaactttc ctggcccgcg ccacatacgc cgaatccgta tccacaccaa gccggcgaag
      181 ccgcgacacc atatgcgccg ggactgtaag aacaacggtg taactctgaa aggagatacg
      241 ccactatgac tgatgtgatt gatcgtgaga cagagacgaa ctcggctcag ccgggtatca
      301 gcgatgcgcg gctcaaagag ctggtcgagc aggcgcgagc cgaagggctg cagttgaccg
      361 gagatggtgg attgctgtcg aagctgacca agctagtcgt ggagtccgcg ctcgaaggcg
      421 agatggacga ccacctcgga tacggcaaga gcgacccggc agggaggggc agcggcaact
      481 cccgcaacgg cagacgagcc aagaacgtgt tgaccgaggc cggcccggtc gaactcgagg
      541 tgccccgtga ccgggattcc tccttcgagc cgaagatcgt ggccaagcgg cagcgtcggc
      601 tgtcgggcgt ggaggacatg gtgatctcat tgtcggccaa gggtttgacg actggtgaga
      661 tcgctgcaca cctggccgag gtgtacggag ccgatgtgtc caagcagacg atctcgacga
      721 tcaccgagcg ggtgatggaa ggcatggccg agtggcagaa ccggccgttg gaccctgtgt
      781 acccggtgtt gttcatcgac tgcatccacg tcaagattcg cgagggcaac gtcgctaacc
      841 ggccgatcta cgtcgcgatg ggggtgactg tcgacggcac ccgcgacatc ctcggattgt
      901 gggtcggtga gcacggcgac ggggagggag cgaagtactg gcttcgggtg ttgtccgagc
      961 tgaaaaaccg tggtgtgcag gatgttctga tcgtcgtgtg cgacggtctc aagcatctac
     1021 ctgactcgat cgagcaagtg tggccacaga ccatcgtgca gacttgtgtg gtccatcttc
     1081 tccgtaacac ctacgcctat gcctcccgca aggactggtc cgatatcgcc aaggacctca
     1141 aacccgtcta cacggctccg tcggagcagg ccgcgctcga ccgattcgca gagttcagtg
     1201 acaaatggga gaaacgctat cc
//
LOCUS       DAFNNB010000290         1191 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0491, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000290 DAFNNB010000000
VERSION     DAFNNB010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1191
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0491"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            65..874
                     /locus_tag="L3M60_003547"
     CDS             65..874
                     /locus_tag="L3M60_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HBN6228159.1"
                     /translation="MANTKLLTYFNIKGGIYKTTTSIMTAYELAKDKDKKILLWDLDV
                     QANLTQYVYEINHNDNTTLDILKGISANDAIVKSPNKNYTNIDLIPSDIQMARFEQEL
                     SPLPAREKFLARWYMQNFNTLSEYDYIICDLSPRYDLTAKNVLFLADSIIILIQDKNI
                     SSLRGAELFKQLWDVDRTYFDKEDNIKSTVLVGFEKKKTQISDTFDSYLEGFNDMRDI
                     MLDTYIRKNEFIEKALLKKLSLTDYTKITKEHFSRQEFANMLEELKVKGVL"
     gene            875..>1191
                     /locus_tag="L3M60_003548"
     CDS             875..>1191
                     /locus_tag="L3M60_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016040102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228160.1"
                     /translation="MPKFDGELLSNKEKPVFKRNGTIIQNDSTTTDNKTLNIYNLVNK
                     KSKKVTMSATLDEELVNKLKSFSIDMNSDVSKLLSDILTQILSDVTIKEENLNIYNER
                     NRR"
BASE COUNT          507 a          146 c          185 g          353 t
ORIGIN      
        1 tattataaaa acaatactat gatattatta taatattata aatataataa tgaggtggag
       61 aaaaatggct aatacaaagc tacttacgta ctttaacata aaaggaggaa tatataagac
      121 aacaacgtct ataatgacag catatgaact tgcaaaagat aaagataaaa aaatactctt
      181 atgggactta gatgtacaag caaatctaac acaatatgtt tatgaaataa atcataatga
      241 taatactaca ttagatattt taaaagggat tagcgctaat gatgcaatag tcaagtcgcc
      301 taataaaaat tacactaata tagacttaat tccttccgac atacaaatgg ctagatttga
      361 acaagagcta tcacctttac cagcaagaga gaaattttta gctcgctggt acatgcaaaa
      421 ctttaatact ttgagtgagt atgattatat tatctgtgat ttatctccac gctacgattt
      481 gacagctaaa aatgtacttt tcttagcaga tagtataatt attctaattc aagataaaaa
      541 catttcttct ttaagaggag cagaattatt taaacagctc tgggatgttg atagaaccta
      601 ttttgataaa gaggataata taaaaagtac tgttttggtt ggatttgaga agaagaaaac
      661 tcaaattagt gatactttcg attcttatct ggaagggttt aacgatatga gagatattat
      721 gttagataca tatattagaa aaaacgagtt tatagagaag gcactattga aaaaactatc
      781 tctaacagat tatacaaaaa taactaaaga gcatttcagc agacaagagt ttgctaatat
      841 gttagaagaa ctaaaagtga aaggagtgtt atagatgcca aaattcgacg gagaactttt
      901 atctaataaa gaaaaaccag ttttcaaaag aaatggaaca atcatacaaa atgattctac
      961 aactacagac aataagacat taaacatata taatctagtt aataagaaaa gtaaaaaagt
     1021 aactatgagt gcaacgcttg atgaagaact tgtaaacaaa ttaaaatctt tttctattga
     1081 tatgaacagc gacgttagca aattattaag tgatatacta actcaaattt tatctgatgt
     1141 cactataaaa gaagagaatt taaatatata caatgagaga aatagaagaa a
//
LOCUS       DAFNNB010000291         1185 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0498, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000291 DAFNNB010000000
VERSION     DAFNNB010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0498"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(67..231)
                     /locus_tag="L3M60_003549"
     CDS             complement(67..231)
                     /locus_tag="L3M60_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228161.1"
                     /translation="MNINNVVVRILAERILNKGLNPLKNRPFELDDVTNIEYRKAVDD
                     YINTIEQEIK"
     gene            complement(231..527)
                     /locus_tag="L3M60_003550"
     CDS             complement(231..527)
                     /locus_tag="L3M60_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228162.1"
                     /translation="MNVPNRVIYDQTGRIIFETGESCGDVLPHDKITELHYIDVEYGS
                     IDYTRNKIVGINIETKEPVLEEIPAYISKAEKEKQELENQLLLMTNKELGGGIL"
     gene            complement(542..1165)
                     /locus_tag="L3M60_003551"
     CDS             complement(542..1165)
                     /locus_tag="L3M60_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016034206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228163.1"
                     /translation="MIEKLNESASLSQLITAFENSTNEIKTIKENIVNAVGNPITSND
                     KLSVIPNKLNDILSEKDKTITQLNAKYKVATGTVTSFADDTKIAYPYRTDRAVKPGTW
                     VKISNLDFKPNIFFADFDYYDIEYKNNYKLFLFACRGVTTQGGVDFSSVTAFIRKNSD
                     EHFHANGWLYGNSEGDVYFNNAGVQIPAYSFNSTQRHIYKWYAIKFI"
BASE COUNT          368 a          199 c          136 g          482 t
ORIGIN      
        1 taagtaaaat aaacaaaatt aaaatttttt agtgacctat tctttaagaa gagttactaa
       61 aagttactat tttatttcct gttctattgt atttatataa tcatctactg cttttctgta
      121 ctctatgtta gtcacgtcat ctaactcaaa aggtctgttt ttcaaagggt tcagtccttt
      181 gtttaaaatt ctttctgcta atattcttac tacaacatta tttatattca ttataaaata
      241 cctccaccta gttctttatt tgtcattaaa agtaattgat tttctaattc ttgtttttcc
      301 ttttctgctt tacttatata tgctggaatt tcttctaaga caggttcttt tgtttctata
      361 tttatgccta caatcttatt tcttgtataa tctatacttc catattcaac atctatataa
      421 tgcagttcag ttattttatc gtgtggcaac acatccccgc aggactcgcc tgtttcaaag
      481 attattctac ctgtctggtc atatattact ctgtttggaa cattcatgtt ttttcctcct
      541 tttaaatgaa tttaatagca taccatttat aaatatgtct ttgagttgag ttaaagctat
      601 aggctggaat ttgaacacca gcattattaa aataaacatc cccttcggaa ttaccataga
      661 gccatccatt ggcatgaaaa tgctcatcac tattttttct aataaaagct gtaacacttg
      721 aaaaatcaac acctccttgt gtagtgacac cacgacaagc gaaaaggaaa agtttataat
      781 tatttttata ttcaatatca tagtaatcaa aatcagcaaa aaaaatatta ggtttaaaat
      841 ctaaattact aattttaacc caagtaccag gcttaacagc tctatcagtt cgatatggat
      901 aagcaatttt agtatcatct gcaaaagaag taactgttcc agttgcaacc ttgtacttag
      961 catttaattg agttatagtt ttatcttttt cagataatat gtcatttaat ttgtttggaa
     1021 ttacactcaa tttatcattt gatgtaattg gatttcctac tgcatttact atattttctt
     1081 taattgtttt aatctcattt gtactatttt caaaagcggt tattaattga cttaaacttg
     1141 cactttcgtt taatttttct atcataaaaa tatcaccttt ctatt
//
LOCUS       DAFNNB010000292         1175 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0502, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000292 DAFNNB010000000
VERSION     DAFNNB010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0502"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            234..527
                     /locus_tag="L3M60_003552"
     CDS             234..527
                     /locus_tag="L3M60_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBN6228164.1"
                     /translation="MGFPETFKALSDPIRREILVMLRENKMSAGEISEKFNMTNATIS
                     YHLSQLKKAGLIFETKYKNFIFYEINISVFEEVMLWFSQFMEKGEKDNEKSDK"
     gene            508..1149
                     /locus_tag="L3M60_003553"
     CDS             508..1149
                     /locus_tag="L3M60_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003435225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HBN6228165.1"
                     /translation="MKKAINKQFILSTLICFIPFIVSIYFYNRLPNEVAIHFDNYGNP
                     DNYAPKVIAAFGVPLLMLCIHLYTWFRLENEPKKANFSNVIQNLSKWGVAILSVILQI
                     MMVLYSTGVSLESNFYTGLILGIIVILFGNYLPKCKQNYSVGIILPWTLNDEDNWNKT
                     HHLAGWIWLIGGILLIINAFINIPFYNIFVIFVIVILPFIYSYLMYKISGKNN"
BASE COUNT          446 a          114 c          178 g          437 t
ORIGIN      
        1 gttagaattt tgatttaaca attgtgatgc agtacacttt tgttaaatta aaaaattaga
       61 taaaataaga attaaataaa agcgacttaa gtaaattgag aatcttaata tgaaaatttt
      121 caataagtat ttagaaataa ctatttagaa atttttctaa atagttattg aaataaaatg
      181 gatgttatgt tatattctat ttagatgaaa ttctaaatag tgaggtgatg gtgttggggt
      241 ttccagaaac attcaaagct ctttcagacc ctataagaag agaaatattg gttatgttga
      301 gggagaataa aatgtcagca ggagaaatta gtgaaaaatt taatatgacg aatgctacaa
      361 tttcttatca tctatctcaa ctaaaaaaag caggactcat atttgaaaca aagtataaga
      421 actttatttt ctacgaaata aatatttcag ttttcgaaga ggttatgctt tggttttcac
      481 aatttatgga gaagggagaa aaagataatg aaaaaagcga taaataaaca atttatttta
      541 tctactttaa tatgttttat accatttatt gtatcaatat atttttataa tagattacca
      601 aatgaagttg caattcattt tgataattat ggaaatccag ataattatgc tccaaaggtt
      661 atagcagctt ttggtgtacc attgttaatg ctatgtatcc atttgtatac atggtttaga
      721 cttgagaatg agcctaagaa agctaatttt tcaaatgtta tacaaaattt atcaaaatgg
      781 ggagttgcta tactttcagt aattctacaa ataatgatgg ttttatattc aacaggagtt
      841 agtttggaat ctaattttta tacgggatta attttaggga taatagttat tttatttgga
      901 aactatcttc ctaaatgtaa acaaaattac tcagtaggaa taattttgcc atggactctt
      961 aatgatgaag ataattggaa taagacacac catttagctg gatggatttg gcttattggt
     1021 ggaatattat taattattaa tgcatttatt aatattcctt tttataatat ctttgtaatc
     1081 tttgtaattg taatactacc atttatatat tcttatttga tgtataaaat ttcaggtaaa
     1141 aataactaat ataaaaaata actaatataa ttaat
//
LOCUS       DAFNNB010000293         1152 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0529, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000293 DAFNNB010000000
VERSION     DAFNNB010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0529"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            23..1152
                     /locus_tag="L3M60_003554"
                     /pseudo
     CDS             23..1152
                     /locus_tag="L3M60_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
BASE COUNT          448 a          129 c          191 g          384 t
ORIGIN      
        1 actttagtca tgggaggttc agttgttgct atctataatt gatgatggaa ttatatgcac
       61 aaataatgat ggcataattc aattctataa ccatatggct agaaaaattt tatctataaa
      121 tgcaaatgaa atgattgact catttgttgg tgattgcata aaagacatta attttcaaaa
      181 catattgacc aataaaactc cattttttga gaaactgatt aaaataaatc atattgatat
      241 aaatttagag attaaacata tacaattaaa tgtatttgat ggatttatac tgaagatgac
      301 aaagttttct caattagaaa aaaagcaagc taaattaaga gcacagctag taaactctgg
      361 taatatcagt aaatatacat ttgatgacat attaggttct agcatacaaa ctattaatac
      421 aaaaaaaatt gctaacaaaa tggctcaatc aaattcatct atccttataa ttggtgaaag
      481 tggcactggt aaagaattat ttgctcaatc cattcatagt gcttctagga gaaaagatgg
      541 tccttttgta gcagtaaatt gtagtacttt tcaagaaaat cttctacaaa gtgaattatt
      601 tggctatgat gaaggggctt ttactggtgc taaaaaaggt ggaaaaattg gtttatttga
      661 attagccaac aatggaacta tatttttaga tgaaattgga gaaatggatt taaattctca
      721 atctaaatta cttagagtca tacaagaaaa acaagtacga cgtattggtt ctaataatgt
      781 cattgatatt gatgtaagaa taattgcagc tactaataga aatttaaaag aacttgtatc
      841 taaaaacatg tttagaagag atttatactt taggttgaat gttcttcccc ttaaaataca
      901 tccacttagg gaaagaccta ctgatatatt tgaaattttt ggttcattaa aatatgatat
      961 tccatgtaat tttagtctaa gtgaagaagt aaaagaaatc tttaaaatgt acaggtggga
     1021 aggaaatgta agagaattaa gaaacttagg agaatacttc tgttacttgg gaaaagatat
     1081 tataaaaata tgtgatttgc ctgagtatat attggatact atagattcaa attatagcag
     1141 aactgtttgt aa
//
LOCUS       DAFNNB010000294         1147 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0535, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000294 DAFNNB010000000
VERSION     DAFNNB010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0535"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            154..732
                     /locus_tag="L3M60_003555"
     CDS             154..732
                     /locus_tag="L3M60_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6228166.1"
                     /translation="MPTKGEQTKQFIIDTSIMLFSEKGYSNVTMKDICEACNLSRGGL
                     YRHFNSPKEIFISILNTHKHYSQNLLDKGIKENISAKHMLEYFFQQQKADINGKYCGF
                     DFAIHEFAFIEKDQRDYMNKRYSSAITMLSNLIDYGQSKSEFKKCDSKSVAIHIMFLF
                     DSMKTSASILKLTNKDIDNQIKIIKDMVILYE"
     gene            725..1027
                     /locus_tag="L3M60_003556"
                     /pseudo
     CDS             725..1027
                     /locus_tag="L3M60_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895500.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase CAT"
BASE COUNT          454 a          165 c          152 g          376 t
ORIGIN      
        1 ccattatatt cttttgtatt cttcaactat tttataaatt aaccttattg tattgtaatt
       61 attttatagt atataacttt aacttgacaa aacgacacaa atgtctcata attaaaagtg
      121 acacttgtgt cgttaaaaag gaggttaaaa catatgccaa caaaaggaga acaaactaaa
      181 caatttatca ttgatacatc aataatgcta ttttctgaaa aaggatatag taatgtgaca
      241 atgaaagata tatgtgaagc atgtaattta agccgtggtg gtctatatag acactttaat
      301 tcaccaaaag aaatttttat cagtatatta aatactcata aacactactc tcaaaattta
      361 ttggataaag gtatcaagga aaatatatca gctaagcata tgctagaata ttttttccaa
      421 caacaaaagg ctgacataaa tggcaaatac tgtggatttg attttgcaat acacgaattt
      481 gcttttatag aaaaagacca acgtgattat atgaataaaa gatacagttc agctatcaca
      541 atgctttcaa atttaatcga ttatggacaa tctaaaagtg aatttaaaaa atgtgattct
      601 aaatcagttg caatacacat aatgttctta tttgatagca tgaaaacatc tgcttccatt
      661 ttgaaactta caaataaaga cattgataat caaataaaga ttataaagga catggtgatt
      721 ttatatgaat aaacaaaaat tctataaaat tgatttacaa aactgggaac gatatacaca
      781 tttttactac ttttcaaaaa tgacaaatgt agggtttaat ttgactatag aaatagatat
      841 tactaattta cttaaggaaa cgaaaaaaag aaatattaaa ttttatccag cgtatttata
      901 cacaataact acttgcttaa atcgatatag agaactaaaa atcgcatatg atgaagatga
      961 acttggatat tggaacacgt taacgccact ttatgcgatt tttcatgatg ataataagac
     1021 tttctcctga acctccaatg actaaagtca cagagttcct gcttcataga attttgcata
     1081 ctaaaatatg tcttactaat tctccacagg ctatccccat agttcctacg gttcttacat
     1141 aagttat
//
LOCUS       DAFNNB010000295         1144 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0537, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000295 DAFNNB010000000
VERSION     DAFNNB010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0537"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>1144)
                     /locus_tag="L3M60_003557"
     CDS             complement(<1..>1144)
                     /locus_tag="L3M60_003557"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012602.1,HMM:NF016750.1,HMM:NF022058.1,
                     HMM:NF024247.1,HMM:NF024900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HBN6228167.1"
                     /translation="ITLTIDGNEITVPEGTSIMRASMEAGIKVPKLCATDMVDAFGSC
                     RLCLIEIEGRNGTPASCTTPAMEGLVVHTQTQRLKDIRKGVMELYISDHPLDCLTCAA
                     NGDCELQDMAGAVGLRDVRYGYQGDNHVFAKADNVANDRWLPKDQSNPYFTYDPSKCI
                     VCSRCVRACEEVQGTFALTIEGRGFSSRVSPGMHEHFIDSECVSCGACVQACPTATLT
                     EKSVIQIGQPEHSKVTTCAYCGVGCSFKAEMRGEELVRMVPYKDGKANRGHSCVKGRF
                     AYGYSTHKDRILNPMIREKISDPWREVSWDEAYKHVANEFRRIQYQYGRNSVGGITSS
                     RCTNEETYLVQKLVRAGFGNNNVDTCARVCHSPTGYGLNKTFGTSAG"
BASE COUNT          222 a          323 c          364 g          235 t
ORIGIN      
        1 gccggcggat gtgccgaatg tcttgttcag gccgtaaccg gtcggcgaat ggcagacgcg
       61 ggcgcaggta tcgacattgt tgttgccgaa accggcacga accagcttct gcacgaggta
      121 ggtttcctcg ttggtgcaac gcgacgaggt gatgccgccg acggagttgc ggccatactg
      181 gtactggatg cggcggaatt cattggcaac gtgcttgtag gcctcatccc aggagacttc
      241 gcgccacgga tcggaaatct tctcgcggat catcgggttg aggatgcgat ccttgtgggt
      301 tgagtagcca taggcaaacc ggcccttgac gcaggaatga ccgcgattgg ccttgccgtc
      361 cttgtaggga accatgcgca ccagttcctc gccgcgcatt tccgccttga acgagcaacc
      421 gacaccgcaa taggcgcagg tggtcacttt ggaatgttcc ggctggccga tctggataac
      481 cgacttttcc gtcagcgttg cggtcgggca ggcctgcacg caggcgccgc aggaaacgca
      541 ctcgctgtcg atgaaatgtt cgtgcatgcc gggtgaaacg cggctggaaa aaccccggcc
      601 ttcgatggtc agcgcaaacg tgccctgcac ttcctcgcaa gcccgcacgc aacgcgagca
      661 gacaatgcat ttcgacgggt cataggtgaa atagggattg gattggtcct tgggcagcca
      721 gcggtcattg gcgacgttgt ccgccttggc aaagacgtgg ttgtcgccct gatagccgta
      781 acgtacatcg cgcaagccga ccgcaccggc catgtcctgc agttcgcaat cgccattggc
      841 ggcgcaggtc aggcagtcca gcggatgatc ggaaatatag agctccatca cacctttgcg
      901 gatgtccttc aggcgctggg tctgggtgtg aacgacaaga ccttccattg ccggggttgt
      961 gcaggaggca ggcgtgccgt tgcgcccttc gatctcgatc aggcaaagac ggcaggaacc
     1021 gaaggcatcg accatgtcgg tggcgcagag tttcggcacc ttgatgcctg cttccatcga
     1081 ggcacgcatg atcgaggtgc cctcgggtac cgtgatctcg ttgccgtcga tggtcagggt
     1141 gatc
//
LOCUS       DAFNNB010000296         1121 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0551, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000296 DAFNNB010000000
VERSION     DAFNNB010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1121
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0551"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            56..1015
                     /locus_tag="L3M60_003558"
     CDS             56..1015
                     /locus_tag="L3M60_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HBN6228168.1"
                     /translation="MISTNVINKVNGITKDNVTNNRVYGADRHETNANVIKKFYTDDE
                     LEAILVAKSDVLVDALAAGPLAASLKSPILITPKTYVSAYHKTNLETKSANKVYKIGG
                     GLTSKVMSSIASSLSKHNTTPADPGSNNGAKTVMIDPGHGGSAPGNSFGGMIEKDYNL
                     NTSLATTEYLRSKDINVIMTRDTDKTLSLGSRTALSNSLKPDLFTSIHYNGDNNRKGH
                     GVEVFYKFKDKNGGTTKTVATNILNRILEKFKLTNRGIKTRVLPSDSTKDYLYVLRTN
                     NIPAVLVECAFLDNEKDVSLINSPAKVKEMGIQIGKGIEDSLK"
BASE COUNT          461 a          137 c          195 g          328 t
ORIGIN      
        1 taaatggcta gagaatgagg atttacaaaa tgcttatttt ataggtggtc ctcaaatgat
       61 atcaacaaat gttataaaca aggtaaatgg aataactaag gataacgtta ctaataatag
      121 ggtgtatgga gcagatagac atgaaacaaa tgcaaatgta ataaaaaaat tctatacaga
      181 tgatgagtta gaagctattt tagtagctaa atcagatgta cttgttgatg ctttagcagc
      241 aggtccattg gctgctagtt taaaatcgcc aatacttata acaccaaaga cgtatgtatc
      301 tgcatatcat aaaacgaatt tagaaactaa atcagctaat aaggtatata aaataggtgg
      361 aggattgact tctaaggtaa tgagttctat agcatcatca ttatctaaac ataatacaac
      421 tccagcagac ccaggaagta ataatggtgc taagacagta atgattgacc cagggcatgg
      481 tggttcagca cctggaaatt catttggagg aatgattgaa aaagattaca atttaaatac
      541 ttcacttgca actactgaat atttacgttc aaaggatata aatgtaatta tgactagaga
      601 tacagataaa accttatctc ttggaagtag aactgcttta tctaattcac ttaaaccaga
      661 cttatttaca agtatacatt acaatggtga taataataga aaaggtcatg gagtagaagt
      721 cttttataag tttaaagata aaaatggagg aactactaag actgtagcta caaacatatt
      781 aaatagaatt ttagagaaat ttaaacttac aaacagaggc ataaaaacaa gagtacttcc
      841 tagtgactct acaaaagatt atttatacgt tttaagaact aataatatac cagctgtact
      901 tgtagaatgt gcatttttgg ataatgagaa agatgtgagt ttaataaact cacctgcgaa
      961 agtaaaagaa atgggtatac aaataggcaa aggaatagaa gattcattaa aataaataga
     1021 aataaaaatt atttaaaaac ttaatgtaaa tttatactaa agttataaaa tgaaatataa
     1081 gtgtatcaat agtagagact gtatgtacat ttgcatatat g
//
LOCUS       DAFNNB010000297         1030 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0638, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000297 DAFNNB010000000
VERSION     DAFNNB010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1030
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0638"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>1030)
                     /locus_tag="L3M60_003559"
     CDS             complement(<1..>1030)
                     /locus_tag="L3M60_003559"
                     /inference="COORDINATES: protein
                     motif:HMM:NF013625.1,HMM:NF015679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HBN6228169.1"
                     /translation="DIADAIVAYYALKQQPIWTSSYDVSAKAKAVAAVFNAAGADGLD
                     PKDYAVKLPSDDYDRANIPARLKELADFELTMSARALRYAMDQGDGRINPNRLSGFHD
                     FAVDRVKPRAVIEQLASTNDAAALLKSFEPQNKWYAELKQNLHELGGTSAEPTVRVAP
                     GTMIRPGEENPEIRNVVALIKAKAPKDFLAKHEAVLQEHENATVYDPSLVEAIKDFQT
                     SQGKKPDGVIGRGTISSLQGETNSSKRDRILYSMERLRWLPHDFGKRYVFINQPAYRA
                     QYFDDNAEKLAMNIVVGSPTNQTYFFDNTIQTVVFNPSWGVPRSIILNEMMPKILNNP
                     SYLENSGYE"
BASE COUNT          196 a          314 c          295 g          225 t
ORIGIN      
        1 cctcgtaacc gctgttctcg agataagagg ggttattgag aattttcggc atcatctcat
       61 tgagaatgat cgaccgcggc acgccccagg acgggttgaa caccacggtc tggatcgtgt
      121 tgtcgaaaaa gtaggtctgg ttggtcggcg agcccaccac gatgttcatc gccagctttt
      181 ccgcattgtc gtcaaaatac tgcgcgcgat aggcaggctg gttgatgaag acgtagcgct
      241 tgccgaaatc atgcggcaac cagcgcaggc gctccatgga atagagaatg cggtcgcgct
      301 tggaactgtt ggtctcgccc tgcagcgaac tgatggtacc gcgcccgatc acgccatcgg
      361 gcttcttgcc ctggctggtc tggaaatcct tgatcgcctc gaccaggctc gggtcgtaaa
      421 ccgtcgcatt ctcatgttcc tgcagcacgg cttcatgctt ggcgagaaaa tccttcggcg
      481 ccttcgcctt gatcaaggcg accacgttgc ggatttccgg gttttcctcg ccaggacgga
      541 tcatggtgcc gggcgcaacg cgcactgtcg gctcggcgct ggtcccgcca agctcatgca
      601 gattctgctt cagttccgca taccatttgt tctgcggctc aaaactcttg agcagcgcag
      661 cggcatcatt ggtcgacgca agctgctcga tcaccgcacg cggcttgacc cggtcaacgg
      721 cgaaatcgtg gaacccgctc agccggttcg gattgatgcg cccgtcaccc tggtccatcg
      781 catagcgcag cgcccgcgcc gacatggtca gttcgaaatc cgccagttcc ttcaaccgcg
      841 ccgggatgtt ggcccggtca taatcatcgc ttggcagctt gaccgcataa tcctttggat
      901 caagcccgtc ggcgcccgcc gcgttgaaca ccgccgcgac cgccttggcc ttggcggaaa
      961 catcatagga ggacgtccag atgggctgct gcttcagggc ataataggca acgatagcgt
     1021 ccgctatgtc
//
LOCUS       DAFNNB010000298         1029 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0641, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000298 DAFNNB010000000
VERSION     DAFNNB010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0641"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            194..535
                     /locus_tag="L3M60_003560"
     CDS             194..535
                     /locus_tag="L3M60_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228170.1"
                     /translation="MLNKKNVSLIALVIILSLFTVIFVFNNRNTNDKLKEVTKNNEEI
                     VPKNLEDLSIKQLTEIRDKAIDKNLEGLREFGEDIVKKRKDLILNFEFGLVAFVNKNY
                     SNLTEKEKLIR"
     gene            575..919
                     /locus_tag="L3M60_003561"
     CDS             575..919
                     /locus_tag="L3M60_003561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228171.1"
                     /translation="MDPSGSFFELANQSYEINEFMLKNKKNYKEWSYEYIEFLIDHLE
                     ELCKFVDFDVKDVIDIIDPTIKTDLSDEQQKSLNDKLKKMSSSETLNEKIKKEIKNWE
                     NNLNSLNMNKNW"
BASE COUNT          468 a           78 c          136 g          347 t
ORIGIN      
        1 ttttatgatt taaaaattta ataaaaaaat tatatacata tgtctaaaaa ttagtttatc
       61 attcgttata attatataaa ctatatcaaa aaagtgttat attgtagtct ttataataag
      121 cagtaataaa ggttactcta ataaactgaa ttatttttta caaacaaaat gcttattaaa
      181 aaggaggttt aatatgttaa ataaaaaaaa tgtaagttta attgctttag taataatttt
      241 aagtctattt actgttatat ttgttttcaa taatagaaat acaaatgata aattaaaaga
      301 agttactaag aataatgaag aaatagttcc taaaaactta gaagatttaa gcataaaaca
      361 attaacagaa attagggata aggctattga taaaaactta gaaggactta gagagtttgg
      421 agaagatata gtaaaaaaac gtaaagattt aattttaaat tttgaatttg gattagttgc
      481 ttttgttaac aagaattata gtaacttaac agaaaaagag aaattaataa gataaattaa
      541 tattgttaac tatgaaagct ctataaatga aataatggat ccttcaggtt cattctttga
      601 attagcaaat caaagttatg aaattaatga gttcatgtta aaaaacaaaa aaaattataa
      661 agaatggtca tatgaatata tagaattcct aattgatcat ttagaagaat tgtgcaaatt
      721 tgttgatttc gatgtaaaag atgtaataga tattattgat cctacaataa aaacagatct
      781 ttcagatgaa caacagaaat ccttgaatga taaattaaag aaaatgtctt caagtgaaac
      841 tttaaatgaa aaaattaaaa aggagataaa aaattgggaa aacaatttaa atagtttaaa
      901 tatgaacaaa aattggtaat atctcaaaat aagtctggaa atatttatgt gtattcttta
      961 actaaaaatt tattttttaa tagaaataaa gcaagattaa aagttgcaag acttcaagaa
     1021 catatagct
//
LOCUS       DAFNNB010000299         1024 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0648, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000299 DAFNNB010000000
VERSION     DAFNNB010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0648"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            234..>1024
                     /locus_tag="L3M60_003562"
     CDS             234..>1024
                     /locus_tag="L3M60_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003422792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HBN6228172.1"
                     /translation="MISNKMQTLVANSSVIRAMFEEGKKLSDIYGEENVFDFSIGNPS
                     VEPPETIKAVINDILNEESPNLVHGYMNNSGYEDVRDAIAEHINKKDGLNLTKENLIM
                     TCGAAGGLNIILKTLLNPGDEVIAFAPYFGEYKNYTENYDGKLIEVPTNIETFEPDLD
                     ALENAITPKTRALIINTPNNPTGVIYSEELLKNLGELLDSKQKEFNTNIYLISDEPYR
                     EIIYDGAKVPCVLKYYRNSFIGYSYSKSLSLPGERIGYIVANGQM"
BASE COUNT          414 a          114 c          147 g          349 t
ORIGIN      
        1 caagaatatt ttattatcta aatctatatt ttattaattc tttacaaaac tattatataa
       61 aataatcatg attaaattag aaattggaat ttttaatatg tactatacat aaatatattt
      121 tatataaaag tttatatttt caaacaaata attttccgaa aattgtctaa attcagtaaa
      181 aatatggtaa tatatacata agcaatataa atctataaaa aggagtgtta catatgatat
      241 ctaataaaat gcaaacttta gttgcaaata gttccgttat aagagctatg tttgaagaag
      301 gaaagaaact ttctgatatt tatggtgaag aaaatgtttt tgattttagt ataggaaatc
      361 caagtgttga acctccagag actattaaag cagttataaa tgatatttta aatgaagaat
      421 ctccaaattt agttcatgga tatatgaaca actcaggata tgaggatgta agagatgcta
      481 ttgctgaaca tataaataaa aaagatggat taaatcttac aaaagaaaat ttaattatga
      541 cttgtggtgc tgctggtggt ctaaatatta ttttaaaaac tcttttaaac cctggtgatg
      601 aagttattgc ttttgcaccg tattttggtg agtataaaaa ttatacagaa aactatgatg
      661 gaaaattaat agaagttcct acaaatatag agacttttga accagattta gatgctctag
      721 aaaatgctat tacaccaaaa acaagagctt taataataaa cactccaaat aatcctactg
      781 gtgtcatata ttctgaagaa ttattaaaaa atcttggaga attattggat tcaaaacaaa
      841 aagaatttaa tacaaatata tacctaattt ctgacgagcc atatagagaa ataatttatg
      901 atggtgccaa agttccttgc gtactaaaat actatagaaa ttcatttata ggatactctt
      961 atagtaaatc attatctcta cctggtgaac gtataggata tattgttgct aatggacaaa
     1021 tgga
//
LOCUS       DAFNNB010000300         1016 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0657, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000300 DAFNNB010000000
VERSION     DAFNNB010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0657"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..824
                     /gene="iolG"
                     /locus_tag="L3M60_003563"
     CDS             <1..824
                     /gene="iolG"
                     /locus_tag="L3M60_003563"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HBN6228173.1"
                     /translation="IEKAGDIDAVIICTPTDTHADLIERFARAGKAIFCEKPIDLNVK
                     RVAECLAVVDETKATLMVGFNRRFDPHFAAVRKAIDDGAIGTVEMVTITSRDPGAPPA
                     DYITRSGGIFRDMTIHDFDMARFLLGEDPVAVTAHASVLVDKKIGELGDYDSVSVILS
                     TASGKQCVISNSRRATYGYDQRIEVHGSKGMVAAENQRPVSIEVANGNGYTRPPLHDF
                     FMTRYTEAYANEIAAFIDAIATGAKAAPSGKDGLIALALADAALRSVKEGKTISL"
BASE COUNT          204 a          306 c          302 g          204 t
ORIGIN      
        1 agatcgaaaa ggccggggat atcgatgcgg tcatcatctg cacgccgacc gatacgcatg
       61 cggacctgat cgagcgcttt gcccgtgccg gcaaggcgat cttctgcgaa aagccgattg
      121 atctcaatgt gaagcgcgtc gcggaatgcc ttgccgtcgt cgatgaaacc aaggccaccc
      181 tgatggtcgg tttcaaccgc cgcttcgatc cgcattttgc cgccgtgcgc aaggccatcg
      241 atgacggtgc catcggtacg gtcgaaatgg tgacgatcac ttcgcgtgat ccgggtgcac
      301 cgccagccga ctacatcacc cgttccggcg gcattttccg cgacatgacg atccatgatt
      361 tcgacatggc gcgctttctg ctgggcgagg acccggtggc ggtgaccgcc catgcttcgg
      421 ttctggtgga caagaagatc ggtgaactcg gcgattatga tagcgtcagc gtcattctct
      481 ccaccgcctc cggcaagcag tgcgtcatct ccaattcgcg ccgcgccacc tatggctatg
      541 accagcgcat cgaagtgcat ggctccaagg gcatggttgc cgcggagaac cagcgtccgg
      601 tgtcgatcga agtcgccaat ggcaacggtt atacccgtcc gccgctgcat gatttcttca
      661 tgacccgcta cacggaagcc tacgccaacg agatagccgc tttcattgac gcgattgcga
      721 cgggcgccaa ggccgctccc agcggcaagg atggcttgat cgccctggcg ttggccgatg
      781 ccgccttgcg gtcggtcaag gaaggcaaga cgatcagtct ctgagtttca atgaaaaacg
      841 ccctgccggt gcggtcaccg gcagggcggt tatatgcagg gtggtcgtgc gtggttcgac
      901 aagctcacca tgagggagag cgaggattgc agggcgcact attctgcaac ctatcaggtt
      961 ggcattgcag ccaaaccacc tccctcatgg tgagcttgtc gaaccacgca ccgcaa
//
LOCUS       DAFNNB010000301         1000 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0682, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000301 DAFNNB010000000
VERSION     DAFNNB010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0682"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            101..>1000
                     /locus_tag="L3M60_003564"
     CDS             101..>1000
                     /locus_tag="L3M60_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009906057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6228174.1"
                     /translation="MKCTNKKILILSLSIITILASVGEVNALEVKNNSNLEKQTIKET
                     VVPELVGLKMQEAFPDENFRKCISDILNIKDSGDTVITDEMKKTIESTTILYITTESI
                     KDLTGINYFTGLKELHCSSNELKELDVSKNTKLRNLYCDNNRLEKLDITNNKNLVHYS
                     WGNQKEPEKPSGGGSYTPTPIVTKINSLVGSDRYATSIKISKQCWDNANNVVLINNSS
                     IADALSATPFAKVKNAPILLTQNNSLNQLTENEISRLGAKNIYIIGGFNSIDESIENY
                     LKDKGLNTIRISGNDRYDTSIKLA"
BASE COUNT          439 a          107 c          148 g          306 t
ORIGIN      
        1 taattaatca tagataagat atatttttga attattgcta aatttatcaa aagatgataa
       61 aatcaggaaa tgtgtattat ttttatagaa ggaatattaa gtgaaatgta ctaataaaaa
      121 aatacttatt ttatcattat cgataataac aattttggct tcagtaggag aagtaaatgc
      181 actagaagtc aaaaataata gcaatttaga aaaacaaaca ataaaggaga ctgttgtacc
      241 tgagttagta ggattaaaaa tgcaagaagc ttttccagat gaaaatttta gaaaatgtat
      301 ttctgatatt ttgaatatta aagatagtgg tgatactgtt ataaccgacg aaatgaaaaa
      361 aactattgaa agcactacaa tattatacat aacgactgaa tctataaaag atttaacagg
      421 aattaattat tttactggac taaaagaatt acattgttct tctaatgaac taaaggaatt
      481 agatgtaagt aaaaatacaa aacttagaaa tttatattgt gataacaata gactagaaaa
      541 attagatatt actaacaata aaaatcttgt acattactct tggggaaacc agaaagagcc
      601 agagaaaccg tctggaggag gttcttatac tccaactcca atagttacaa aaataaattc
      661 tttagtaggt tctgatagat atgctacctc aataaaaata agtaaacaat gctgggataa
      721 tgcaaataat gtagtactta taaataattc tagtatagca gatgcattat cagctacacc
      781 atttgctaaa gttaaaaatg caccaatatt acttactcaa aataatagtt tgaatcaatt
      841 gacagaaaat gaaataagta gattaggtgc aaaaaacata tacataattg gtggatttaa
      901 ttctatagat gaaagtattg aaaattatct aaaagataaa ggtttaaata caataagaat
      961 atctggaaat gatagatatg atacttcaat aaaactagca
//
LOCUS       DAFNNB010000302          994 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0689, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000302 DAFNNB010000000
VERSION     DAFNNB010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0689"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            278..>994
                     /locus_tag="L3M60_003565"
     CDS             278..>994
                     /locus_tag="L3M60_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6228175.1"
                     /translation="MRRNTKLLTTGILSMAIVAPTMAFATESNAMENNADLNINLEKK
                     SIVLGSTSKVSVKFKERPDADSITLKYKCYDMPLDTTLNYNQSTDSYEGTINYNKDPE
                     YLNVWELQGITINSKNNPKTLNKQDLENMGLNLKDYNVTQECIIEDITSRKDVNKYLR
                     KTSAPITELTGSDRYETAVKISKEGWKNGSDKVVIINGDVSIDGIISTPLATTYNAPI
                     LLVEKNNVPNSVKSELKRLNP"
BASE COUNT          405 a          106 c          149 g          334 t
ORIGIN      
        1 atcttttttt gttgtattaa atttaataaa cctacaatta ttatatgttt ttatttgtct
       61 tttatagatt tttttggcaa taaatgatat agtgaagata attaaatata ttcaatatat
      121 ttaatttttt tattttttgt ttttataatt tttttagaag tttatttttt attttatgta
      181 cttatttaca attttgggta aatacacttt gatatatttt caaaagttaa aaggttaaaa
      241 aataaagtag aaatttatgt taaataagga gattaatatg agaagaaata caaaattatt
      301 aacaacaggg attctttcaa tggcaatcgt cgcacctaca atggcatttg ctactgaatc
      361 taatgctatg gagaataatg ctgatttaaa tataaactta gagaaaaaaa gtatcgtttt
      421 aggtagtact tcaaaagtca gtgttaagtt taaagaaagg cctgatgcag atagcattac
      481 attaaaatat aaatgctatg atatgccatt ggatacaact ctaaactaca atcaatcaac
      541 tgattcgtat gagggaacta ttaattataa taaagaccca gaatatctaa atgtttggga
      601 actacaaggg ataacaataa acagcaaaaa taatcctaaa actttaaata aacaagacct
      661 agaaaacatg ggattaaatt taaaagacta taatgtaaca caggaatgta taattgaaga
      721 tataacttct agaaaagatg taaataaata tttgagaaaa acttctgcgc ctattacaga
      781 acttacagga agcgatagat atgaaacagc agttaaaata agtaaagagg gttggaaaaa
      841 tggttcagat aaggtagtta taataaatgg agatgtaagt atagatggta ttatatcaac
      901 tccactggca actacatata atgcgccaat acttttggtt gaaaaaaata atgtgcctaa
      961 tagtgtaaaa tcagaattaa aacgtctaaa ccct
//
LOCUS       DAFNNB010000303          978 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0712, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000303 DAFNNB010000000
VERSION     DAFNNB010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0712"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(201..827)
                     /locus_tag="L3M60_003566"
     CDS             complement(201..827)
                     /locus_tag="L3M60_003566"
                     /inference="COORDINATES: protein motif:HMM:NF015531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228176.1"
                     /translation="MPVVCVRSFDAHDYEDAALALLNQYRDEPYLVLDIRGNGGGNTP
                     EKLIKALMNQPYPTWREASSFRVGVDLATQQLQNSNSLRAGEVPRFTLSWNCTVQQPE
                     ENAYNGKLFILHDIGTKSAAEDFLLPFKATARAVLVGETTSGSSGQPYIETPFPDAMF
                     TVGAKRQEFPNGDRFEGIGISPDIAIFASQANTGPGTDFVLKELLKLI"
BASE COUNT          229 a          240 c          231 g          278 t
ORIGIN      
        1 cttgaattcc cttctctaaa actcttaaat tgtgatgcta ttgtatcaag atcgcgctca
       61 ataatggcag ccgtctcatg tccaaattta cattccatag cagcgtatag ctcttgggaa
      121 aaatactcgg aatcattccg atcgtaccaa tagagaactg ctcgcttggt ttcgctgtaa
      181 tctaccatgg tccacttccg ttagatcagt ttcagcaact ctttcagcac gaagtcggta
      241 ccaggccccg tgttggcttg tgaggcgaag atcgcaatat cgggggaaat gcctatgcct
      301 tcaaaacggt cgccattagg aaactcctga cgtttggctc caacagtgaa catcgcgtct
      361 gggaatggtg tttcgatgta gggctgcccg ctacttccgc tcgttgtctc accaactagc
      421 acagctcgag ctgtcgcttt gaatggcaaa agaaagtctt ctgcggccga ttttgtacca
      481 atgtcgtgca gtatgaaaag cttgccattg taggcgtttt cttctggctg ctgaacagtg
      541 cagttccatg acagtgtaaa tctgggaact tcccctgctc ggagactatt tgaattctgc
      601 agttgttgag tggctaaatc cactcctact ctgaaagaag aagcctctcg ccaagtaggg
      661 taaggctgat tcataagcgc cttgataagc ttttcaggtg tgtttccgcc accatttcct
      721 ctaatatcca gcacaagata gggttcgtct ctgtactggt ttagtaaagc gagagccgca
      781 tcctcgtaat cgtgcgcgtc gaaacttcta acgcagacca cgggcatagt gtattcgttt
      841 cgaaattcga caactgcccg tggcgcgtgc ggtggccgcc ccttggatat ctgcttggtt
      901 acgccggatt ctaattcaat ttcgattgtt tctggaagaa agaatggacg tcgccacagt
      961 atgtatccct tgtagctg
//
LOCUS       DAFNNB010000304          977 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0716, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000304 DAFNNB010000000
VERSION     DAFNNB010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..977
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0716"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(114..>977)
                     /locus_tag="L3M60_003567"
     CDS             complement(114..>977)
                     /locus_tag="L3M60_003567"
                     /inference="COORDINATES: protein motif:HMM:NF012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228177.1"
                     /translation="NPIDAYIDPTTPTSPEQLANLRAQLGLDKSLPVQYLAWLQQAVT
                     GNLGFSIKRQDQPVLGLVMGRIGPTVLLMGSALFIAIVAGIVTGVISAVKRNSLTDLS
                     FSVFALVGISSPAFLSALIGLYVFAVRLKWAPAGGMLTPGSAFSLVDIMRHLILPATL
                     LSIAQAALIMRYMRSSMLEVLSQDYVRTARAKGVKEYWVIAKHALRNALLPVVTVIGS
                     TVGLAIGGAIFIESVFNWPGMGLLLVNAVETRDYPVIMGATLVIGICVIVINLLTDIT
                     YAFIDPRIKVG"
BASE COUNT          256 a          266 c          276 g          179 t
ORIGIN      
        1 tcagcagcgg aatgatcaca cagaggccaa aaattgctgc cctgttatgt ccgaagcggt
       61 acagagctct caagatcggt ccgggacttg acgtcaatgc gcgcgcaaac atctcagccc
      121 accttgatac gtggatcgat gaacgcatag gtgatgtcgg tcaaaagatt gatgacgatc
      181 acacagatcc cgatgacaag ggttgcaccc atgatgacag gatagtcacg cgtctccacg
      241 gcattgacca gtaacagtcc catgcccggc cagttaaaga cactttcgat gaaaatcgcg
      301 ccgccgatgg caagaccgac ggtggatccg atcacggtca cgactggcag gagcgcattg
      361 cgcagggcat gtttggcgat gacccaatac tccttgacgc cttttgcccg agcagtgcga
      421 acataatcct gactgagcac ctcaagcatt gacgagcgca tataccgcat gatcagcgca
      481 gcctgggcga ttgaaagaag cgttgcgggc aagatcagat gcctcattat atcaacgagc
      541 gaaaatgcac tgcctggcgt cagcatgccc cctgctggtg cccatttcaa acgcacggca
      601 aagacgtaga gcccgatgag cgcgctcaga aatgccgggc ttgagatgcc aacgagggcg
      661 aaaacggaga atgacaggtc agtgagagag ttgcgcttca cggcgctgat taccccggtg
      721 acgatgccgg cgacaatggc gataaacaat gccgaaccca tcaacagaac ggttggtccg
      781 atccgcccca taacgagccc caaaaccggc tggtcctggc gtttgatgga aaagccgagg
      841 ttccccgtga ccgcctgctg aagccaggca agatactgca cgggcaaaga cttatcgagt
      901 ccaagctgtg cgcgcaggtt ggcaagctgc tccggagatg tcggagtggt cggatcaatg
      961 taagcatcaa ttggatt
//
LOCUS       DAFNNB010000305          916 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0815, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000305 DAFNNB010000000
VERSION     DAFNNB010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..916
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0815"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            56..475
                     /locus_tag="L3M60_003568"
     CDS             56..475
                     /locus_tag="L3M60_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3785 domain-containing protein"
                     /protein_id="HBN6228178.1"
                     /translation="MSLYKFIYDDKEYLLKEENCSALINDEENPVQGVSISKIIDILN
                     EAEEVDFDVEYYQEACPLCLEGVKEKKKFFPFLEYHFYVFSKDGEYVISNISVDYKGL
                     SFNKLSRANKVDNSYIVSVIICENCQDYIIQIENCIV"
     gene            606..>916
                     /locus_tag="L3M60_003569"
     CDS             606..>916
                     /locus_tag="L3M60_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6228179.1"
                     /translation="MEEIDDTILDSMFKLTLNERLLNNNDFIENKIDVVKNLIDCYKT
                     EQNSLMLLKSNFLLALLYGIQGVSEKMKEYILISCKYIDKCLPKDYKFLARFYSQIAI
                     L"
BASE COUNT          365 a           62 c          159 g          330 t
ORIGIN      
        1 ctttatttaa gtggtagaat aacttggaga ttaataatta taaggaggat ataatatgag
       61 tttatataag tttatatatg atgataagga atatttatta aaagaggaga attgtagtgc
      121 tttaataaat gatgaagaaa atccagttca aggagtaagt atttcaaaaa taattgatat
      181 acttaatgaa gcagaagaag ttgattttga tgtagaatat tatcaagaag catgtccatt
      241 atgtttagaa ggggtaaagg aaaagaaaaa attctttcct tttttggaat atcactttta
      301 tgttttcagt aaagatggag aatatgtaat aagtaatata tctgttgatt ataaaggatt
      361 atcttttaat aaactatcaa gggcaaataa agttgataat agttatattg taagtgtgat
      421 aatttgtgaa aattgtcagg attatataat tcagatagaa aattgtatag tttagtgtaa
      481 agtatgtctt aatttaagag aaatttctaa attgagacat cgactttttt caaaaatagt
      541 atggaaaatt actaaattta tgagataatg taagtcggag gtgagttaca atatatagta
      601 aatatttgga agagatagat gatacaattt tagattcaat gtttaagcta accttgaatg
      661 aaagattatt gaataataac gattttattg aaaataaaat tgatgtagta aagaatttaa
      721 tcgattgcta taaaaccgaa caaaatagtt tgatgttatt aaaatcaaat tttttgctag
      781 ctttattata tggtattcaa ggggttagtg aaaaaatgaa agagtatatt ttgatttcct
      841 gtaaatatat tgataaatgc ttacccaaag attataagtt tctagcacga ttttattcac
      901 aaatagctat tttaag
//
LOCUS       DAFNNB010000306          872 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0894, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000306 DAFNNB010000000
VERSION     DAFNNB010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0894"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            19..843
                     /locus_tag="L3M60_003570"
                     /pseudo
     CDS             19..843
                     /locus_tag="L3M60_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
BASE COUNT          353 a          119 c          136 g          264 t
ORIGIN      
        1 aaaactaagg agacctatat gttaaaagta ctaaaaggaa atataatttt cacaaaaact
       61 tcagaaactt tcacagtatt tgaaaatagc tatattatac tagaaaaagg caaagtaaaa
      121 gaaatattta aagaaatacc taaggaatac aaaaatctag aaataataga ttacactgat
      181 aaacttataa tacctggtat gaatgacctc catgcccatg cctctcaatt taaaaatctt
      241 ggaatggcta tggataaaga gcttttacca tggcttgaaa cttatacatt tccagaagaa
      301 tcaaattata aagatatcga atatgcaacg aaaatgtata aaaaatttgt aaaagaactt
      361 tggaaatctg gaactacaag aatagcagtg tttgcaacag tgcataaaga agcctctatg
      421 aaacttatgg atatattcaa agaagttggt cttggagcat acgttggtaa agtaaatatg
      481 aatatgaatt gccctaatac tttgttagaa aacactcatg attctattgc tgatacagaa
      541 gaaattataa ataagtatag tgatacagat tctattgtaa aaccaatagt aacacctaga
      601 tttataccca gctgtacaag tgaactttta aaaggtttag gagatttatg tttaaaatat
      661 aatactccta tgcaatcaca cttatctgaa aattatgatg agattgaatg ggttagaaat
      721 ctagaacctg aatctaaatt ctatggagat gcatataata gatataattt atttggtcaa
      781 actcctactc taatggctca ttgtgttcat tgttctcagg aagaaataga tttgattaaa
      841 aaatagctac gcacacttgt gactttagtc at
//
LOCUS       DAFNNB010000307          857 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0921, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000307 DAFNNB010000000
VERSION     DAFNNB010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..857
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0921"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>857
                     /locus_tag="L3M60_003571"
     CDS             <1..>857
                     /locus_tag="L3M60_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C80"
                     /protein_id="HBN6228180.1"
                     /translation="LININNSLFYFDPIESNLVTGWQTINGKKYYFDINTGAALTSYK
                     IINGKHFYFNNNGVMQLGVFKGPDRFEYFAPANTQNNNIEGQAIVYQSKFLTLNGKKY
                     YFDNDSKAVTGWRIINNEKYYFNPNNAIAAVGLQVIDNNKYYFNPDTAIISKGWQTVN
                     GSRYYFDTDTAIAFNGYKTIDGKHFYFDSDCVVKIGVFSGSNGFEYFAPANTYNNNIE
                     GQAIVYQSKFLTLNGKKYYFDNNSKAVTGWQTIDSKKYYFNTNTAEAATGWQTIDGKK
                     YYFNTNTAE"
BASE COUNT          316 a          107 c          139 g          295 t
ORIGIN      
        1 attaatcaat ataaacaatt cattattcta ttttgatcct atagaatcta acttagtaac
       61 tggatggcaa actatcaatg gtaaaaaata ttattttgat ataaatactg gagcagcttt
      121 aactagttat aaaattatta atggtaaaca cttttatttt aataataatg gtgtgatgca
      181 gttaggagta tttaaaggac ctgatagatt tgagtatttt gcacctgcca atactcagaa
      241 taataacata gaaggtcagg ctatagttta tcaaagtaaa ttcttaactt tgaatggcaa
      301 aaaatattat tttgataatg actcaaaagc agtcactgga tggagaatta ttaacaatga
      361 gaaatattac tttaatccta ataatgctat tgctgcagtc ggattgcaag taattgacaa
      421 taataagtat tatttcaatc ctgacactgc tatcatctca aaaggttggc agactgttaa
      481 tggtagtaga tactactttg atactgatac cgctattgcc tttaatggtt ataaaactat
      541 tgatggtaaa cacttttatt ttgatagtga ttgtgtagtg aaaataggtg tgtttagtgg
      601 ctctaatgga tttgaatatt ttgcacctgc taatacttat aataataaca tagaaggtca
      661 ggctatagtt tatcaaagta aattcttaac tttgaatggt aaaaaatatt actttgataa
      721 taactcaaaa gcagttactg gatggcaaac tattgatagt aaaaaatatt actttaatac
      781 taacactgct gaagcagcta ctggatggca aactattgat ggtaaaaaat attactttaa
      841 tactaacact gctgaag
//
LOCUS       DAFNNB010000308          855 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0926, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000308 DAFNNB010000000
VERSION     DAFNNB010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0926"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..452
                     /locus_tag="L3M60_003572"
     CDS             <1..452
                     /locus_tag="L3M60_003572"
                     /inference="COORDINATES: protein motif:HMM:NF012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228181.1"
                     /translation="LAVFGANQYGIIFALGIAYTPSMARIVRGAVLSLREREFIEASK
                     VMGNSEIYTILRHILPNCLAPITVLGTSMFGWAILSESALSFLGLGVPPPAPTWGNML
                     AAGRPFIEQAAWLGFFPGLCIALTLLGINLLGDALRDKLDPRMRGLK"
     gene            449..>855
                     /locus_tag="L3M60_003573"
     CDS             449..>855
                     /locus_tag="L3M60_003573"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6228182.1"
                     /translation="MSNQNALLSVRGLTLEVIHNGGAVVKDVSFDVSPGEIVGIVGES
                     GSGKTLATRSIIGLIAPGIRHASGSIIYKDQDTLQASDRALRRLRGGEIGVVFQEPMT
                     SLNPSMTIGRQLEEGLVLHTKQTPEMRRTNILH"
BASE COUNT          158 a          272 c          243 g          182 t
ORIGIN      
        1 tgctcgcggt tttcggcgcc aaccagtacg gcatcatctt cgcacttggg atcgcctata
       61 ccccgtcgat ggcgcgtatc gtgcgcggtg ccgtcctttc cctgcgtgaa cgggaattca
      121 tcgaagcctc caaggtcatg ggcaacagcg aaatctatac gattctccgc cacatcctgc
      181 ccaattgcct ggcaccgatt acggttctgg ggacatccat gttcggctgg gcgatccttt
      241 cggaaagtgc actcagcttc ctcggccttg gcgttccgcc acccgcaccc acctggggca
      301 acatgcttgc cgccggccgg ccgttcatcg aacaggcagc ctggctcggc ttcttccccg
      361 gcctttgcat cgcgctgacc cttctcggca tcaacctgct tggagatgcc ctgcgtgaca
      421 agctcgatcc ccgcatgaga ggtttgaaat gagcaatcaa aacgcgcttc tcagcgttcg
      481 cgggctgacg cttgaggtca tccacaatgg cggcgccgtc gtcaaagatg tgagcttcga
      541 tgtctccccc ggagagatcg tcggtatcgt cggtgaatca ggatcgggca agacgctggc
      601 gacgcggtcg atcatcggac tgattgcgcc gggcatccgg catgccagcg gttcgatcat
      661 ttacaaggat caggatacgc ttcaggccag cgaccgcgcg ttgcggcgtt tgcgtggcgg
      721 cgaaatcggc gtcgtcttcc aggagccgat gacctccctc aatccgtcga tgaccattgg
      781 ccgccagttg gaagaagggc tggttctcca taccaagcag acacccgaga tgcgccggac
      841 caacatcctg catat
//
LOCUS       DAFNNB010000309          854 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0930, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000309 DAFNNB010000000
VERSION     DAFNNB010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0930"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..317)
                     /locus_tag="L3M60_003574"
     CDS             complement(<1..317)
                     /locus_tag="L3M60_003574"
                     /inference="COORDINATES: protein motif:HMM:NF013227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HBN6228183.1"
                     /translation="MQFIDLGAQRERISSKLDAAIAKVVREGKYILGPEVAEFEKRLG
                     AYLGVEHVVACANGTDALQIPLMARGIGPGDAVFCPSFTFAATAEVVALVGAEPVFID
                     VDA"
     gene            complement(490..>854)
                     /locus_tag="L3M60_003575"
     CDS             complement(490..>854)
                     /locus_tag="L3M60_003575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228184.1"
                     /translation="HMRFPNNIRGHLFASRLNPYRERRLTVVGTKAMAVFDDMEQWDK
                     KLAVYKHSIWQDNGQWASTTEEPTYVKIAQGMPLTRECQNFIDSINTRSEPVTDGEEA
                     IRVLRILTAGTVAHDKAS"
BASE COUNT          173 a          255 c          245 g          181 t
ORIGIN      
        1 tcggcgtcga catcgatgaa aaccggctct gctccgacaa gcgccacgac ttcagcggtg
       61 gcggcgaagg tgaagctcgg gcagaacacc gcatcgcccg ggccgatgcc gcgggccatc
      121 agcgggatct gcagggcatc cgtgccgttg gcacaggcaa ccacatgctc gacaccgaga
      181 tatgcgccaa gacgcttttc gaactcggca acttccggtc cgagaatata tttgccttca
      241 cgcaccactt tggcgattgc cgcatcaagc ttgctgctaa tccgttcacg ctgtgcgccg
      301 agatcgatga actgcatcat tgctccaata tcaattgttc cgcgaatccg tcgcggaaaa
      361 cggtcaagac aactctttag aagggccgtg ggaggtctgc cagacgttgt cgttcaaagt
      421 ttgtccggtc ttgtaacaac gctttacacc gagcgcaatc ttgcagcact tttggggccg
      481 ccggacggat cagctcgcct tgtcgtgcgc aaccgtgccg gcggtcagaa tccgcaggac
      541 gcgaatcgct tcctcgccat cggtgaccgg ctcgctgcgc gtattgatgc tgtcgatgaa
      601 attctggcat tcccgggtca gcggcatgcc ctgtgcaatc ttgacatagg tcggctcttc
      661 cgtcgtcgac gcccactggc cgttatcctg ccagatcgaa tgcttgtaga cggcaagctt
      721 cttgtcccac tgctccatgt cgtcgaagac ggccatggcc ttggtgccga cgaccgtaag
      781 ccggcgctcg cgatagggat taaggcgcga ggcaaaaaga tgcccgcgaa tattgttggg
      841 aaagcgcatg tgca
//
LOCUS       DAFNNB010000310          842 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0949, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000310 DAFNNB010000000
VERSION     DAFNNB010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0949"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..649
                     /locus_tag="L3M60_003576"
     CDS             <1..649
                     /locus_tag="L3M60_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003439467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HBN6228185.1"
                     /translation="FITIVSSIIEIWSCKKVFDYTSQTKTDLKKLNMYFFFSVFLIIS
                     FLYINIGANSRILICIFVMFIFYKSNYKVNIYKCLVISFFYWLILIGINGLSMLLVAT
                     VSNLDNMNVLLSGSLYRYEAIFLGKLILGILLYIYKISFKNFKISKKEIYICIPMIAN
                     LLSFFVLYDYILNLVKISPERRTAFLVIVLLLILSNISIFVLIHIILKIKIRKIR"
BASE COUNT          327 a           65 c           93 g          357 t
ORIGIN      
        1 atttataaca attgtttctt ctattataga aatatggtca tgtaaaaaag tttttgatta
       61 tacgagtcag acaaagactg acttaaaaaa actaaatatg tatttcttct tttctgtttt
      121 tcttattatt tcttttttat atataaatat aggagctaat agtagaatct tgatatgtat
      181 atttgtaatg ttcattttct ataagtctaa ctataaagtt aatatatata aatgtctagt
      241 gatatcattt ttttattggt taatattaat aggtataaat ggattaagta tgttattagt
      301 agcaacagta agtaatttgg ataatatgaa tgttttactt agtggaagtt tatatagata
      361 tgaagcaata ttcttgggaa agttaatatt aggtatcttg ctgtatattt ataaaatttc
      421 ttttaaaaat tttaaaataa gtaaaaaaga gatatacata tgtattccta tgatagcaaa
      481 tctattgtca ttttttgtac tttatgatta tattcttaat ctcgtaaaaa tttctcctga
      541 aagaagaact gcttttttag taatagtttt actattaata ttatctaaca tttcaatctt
      601 tgtattaata catataatac ttaagattaa aattaggaaa ataagataaa tcttaatagg
      661 gttcatttaa attaaaagtt aataattact tttttatgta taattttaca taaatttgtg
      721 taaaagttat tttattaaag atagatatac ttatatatac agatatgatt aaaaaaaatc
      781 tttttaactt taaaaattaa tgttaaaaaa taaacttata cactaatatt aacaataatt
      841 at
//
LOCUS       DAFNNB010000311          832 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0972, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000311 DAFNNB010000000
VERSION     DAFNNB010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0972"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            14..104
                     /locus_tag="L3M60_003577"
     tRNA            14..104
                     /locus_tag="L3M60_003577"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48..50,aa:Ser,seq:gct)
     gene            113..189
                     /locus_tag="L3M60_003578"
     tRNA            113..189
                     /locus_tag="L3M60_003578"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:147..149,aa:Pro,seq:tgg)
     gene            274..349
                     /locus_tag="L3M60_003579"
     tRNA            274..349
                     /locus_tag="L3M60_003579"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:307..309,aa:Trp,seq:cca)
     gene            410..486
                     /locus_tag="L3M60_003580"
     tRNA            410..486
                     /locus_tag="L3M60_003580"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:444..446,aa:Pro,seq:tgg)
     gene            493..569
                     /locus_tag="L3M60_003581"
     tRNA            493..569
                     /locus_tag="L3M60_003581"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:527..529,aa:Ile,seq:gat)
     gene            573..648
                     /locus_tag="L3M60_003582"
     tRNA            573..648
                     /locus_tag="L3M60_003582"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:606..608,aa:Phe,seq:gaa)
     gene            656..732
                     /locus_tag="L3M60_003583"
     tRNA            656..732
                     /locus_tag="L3M60_003583"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:690..692,aa:Met,seq:cat)
BASE COUNT          219 a          182 c          225 g          206 t
ORIGIN      
        1 aataactgaa tttggagaag tactcaagtg gctcaagagg atcccctgct aagggattag
       61 gctgggtaac cggtgcgagg gttcgaatcc ctccttctcc gccattcaaa aacgaggtgt
      121 agcgcagttt ggtagcgcac atggtttggg accatggggc cgggggttcg agtcccttca
      181 cctcgaccaa cttagctttt agttcggttg gttagagtgc ccaactcata accagagatt
      241 ctggggattg agtccatgaa ggttattctt cataggggtg tagctcagtt ggtagagcgt
      301 cggtctccaa aaccgtgcgc cgggggttcg agtccctcca cccctgccaa ataaaaagga
      361 cttgaaaaaa gttgggtttt agagtagtat aagatagtaa ataagatttc gaggtgtagc
      421 gcagtttggt agcgcacatg gtttgggacc atggggccgg gggttcgagt cccttcacct
      481 cgaccaagaa aagggcctat agctcaggtg gttagagcgc acgcctgata agcgtgaggt
      541 cgctggttcg agtccagcta ggcccaccat ttgcccagat agctcagtcg gtagagcagg
      601 ggactgaaaa tccccgtgtc ggtggttcga ttccgcctct gggcaccaga aatacggcgg
      661 tatagcttag ttggctagag cgttcggttc atacccgaaa ggtcataggt tcgactccta
      721 ttaccgctac catctaacta tatcaataaa gaactttgaa aattaaacag taggttaatt
      781 tataaacaag aaacaaacca taaagccaga tattttgata acaatagtat ct
//
LOCUS       DAFNNB010000312          832 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0974, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000312 DAFNNB010000000
VERSION     DAFNNB010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0974"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(139..>832)
                     /locus_tag="L3M60_003584"
     CDS             complement(139..>832)
                     /locus_tag="L3M60_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6228186.1"
                     /translation="GYSDVFISNAEKLNIDLGNLTHVVFSHGHNDHTRGIQFLENRYD
                     LSTVELISHPNCFIPKKHGEKSIGAPFSAEEIKNIFMYNPKDKPFNLSKNCVFLGEIP
                     SINNFEKRAKIGKCKIGDLWEDDYVLDDSAIICKTGKGIFIVTGCSHSGICNIVEYAK
                     KVCGDDRVIGILGGFHLFELNNRLDSTIQYLDKEEIRMLYPCHCVSFKVKAKMNEILN
                     VNEVGVGLTISI"
BASE COUNT          303 a          156 c           67 g          306 t
ORIGIN      
        1 cttattaaaa ataaattttt tcacaatatt tcatataaaa gtacattgat ttctcttaaa
       61 ataaacataa taaaaaagat actttctata gaaaatatct ttttaacctt caacttaaat
      121 cttatatact ttcaaatact atatgcttat agtcaatcct acccctactt catttacatt
      181 caatatttca ttcatcttag cttttacctt aaaagaaaca caatgacaag ggtacaacat
      241 tcttatttct tctttatcca aatattgtat tgtgctatcc agcctattat tcaattcaaa
      301 aagatgaaaa ccacccagta taccaataac tctatcatct ccacatacct ttttagcata
      361 ctctactata ttacaaattc cactatgaga acatcctgta acaataaaaa tacctttacc
      421 agttttacaa attatagcag agtcatctaa aacataatca tcttcccaca aatctcctat
      481 tttacattta ccaattttag ctcttttttc aaaattattt atagaaggta tttcacctaa
      541 aaacacacaa ttcttactta gattaaatgg cttatcctta ggattataca taaaaatatt
      601 tttaatttcc tcagcagaaa atggagctcc tatgcttttc tctccatgtt ttttaggtat
      661 aaaacagtta ggatgtgaaa taagttcaac tgtagacaaa tcatatctat tttctaaaaa
      721 ctgtatacct cttgtatggt cattatgacc atgagaaaaa acaacatgtg ttaaatttcc
      781 taaatctata tttaattttt cagcgtttga tatgaataca tctgaatatc cc
//
LOCUS       DAFNNB010000313          827 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.0983, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000313 DAFNNB010000000
VERSION     DAFNNB010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.0983"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..247)
                     /locus_tag="L3M60_003585"
     CDS             complement(<1..247)
                     /locus_tag="L3M60_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228187.1"
                     /translation="MINISSNIGLCIYIFIYFITIFLIPVFFRNNNLSFAKSILFTLV
                     IIIISLISIFYLDGLGKSLYRYCVLLLIVGVDIIITFM"
BASE COUNT          366 a           80 c           80 g          301 t
ORIGIN      
        1 tcatgaaagt tataattata tctactccaa caatcaataa taaaacacag tatctatata
       61 acgattttcc tagtccatca aggtaaaaaa tacttataag actaattatt attataacta
      121 atgtaaacaa aatactcttt gcaaatgata aattattatt tctaaaaaat acaggtataa
      181 gaaaaatagt aataaaatat ataaaaatat aaatacataa tccaatatta gaacttatat
      241 ttatcataga tttgcctcct aaataaatag tttattatag ttcaaattat gttctatttt
      301 aattatgata aattttaaga tggtataaaa aataagaggt attaatctct tgttttttta
      361 tgattttctt tataatacat aataatcttt attatatata aagtcaaaag tgttccaaat
      421 ataaatatat ctaattgaaa ttgtccataa acaatactga ataaattatg tgaaatcgtt
      481 aataaaactt gtgggatttg tatgattatt atcaaaaaca tacctataat caaagagact
      541 atatgaaaac tctctttttt ttataaaaac ttatatataa ataatgcaaa tattaaaaag
      601 taaataaatg ttggaaagat tatgtttcta taagctagtt tctaaagagt tattctgaaa
      661 ttagttataa aatttatatt tttgaaactt tatatacaaa ataatagtct aactaatgtg
      721 acaatataaa ggaggctgag atgactaata tattaaaatt taaattatta tccttttcta
      781 aagaaaaaaa agaacatagc ctagaaaaaa aagctattta tggagat
//
LOCUS       DAFNNB010000314          800 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1037, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000314 DAFNNB010000000
VERSION     DAFNNB010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1037"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            86..721
                     /locus_tag="L3M60_003586"
     CDS             86..721
                     /locus_tag="L3M60_003586"
                     /inference="COORDINATES: protein motif:HMM:NF013043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228188.1"
                     /translation="MKIGDKFENLTILDIEQKNSRKYCLCKCKNCGNEKWIRADSLKR
                     IKGCGCLQKETQFKQNDLTNKKFGRLTALKNTNKKAKSGHYIWICKCSCGNEIKTTEN
                     NLTTGRTKSCGCLKKESNIKNAKIALKVHKEKNIIDDTNLSIIKKTEAYPNSKTKIRG
                     VSWNKEKRKYCAQIEFKKTHYNLGYYDNIREAEEAYKKAKEKFLKEINGKV"
BASE COUNT          388 a           80 c          132 g          200 t
ORIGIN      
        1 aaataaatac agtgagttta atcaaattca ctgtatttat ttttacaata gtagaataat
       61 attattagtt caggatgtga taggcttgaa aattggagat aaattcgaaa accttacaat
      121 actagacata gaacaaaaaa acagtaggaa atactgtcta tgtaaatgta aaaattgtgg
      181 caatgaaaag tggataagag cagatagttt aaaaagaatt aaagggtgtg gatgtttgca
      241 aaaagaaaca cagtttaagc aaaatgattt gacaaataaa aagtttggaa gattaacagc
      301 actaaaaaac actaataaaa aagctaaaag tggtcactat atttggattt gtaagtgctc
      361 ttgtggaaat gagattaaga caacagaaaa caatttaact actggcagaa ctaaatcatg
      421 tggatgtttg aaaaaggaat ctaatataaa aaatgcaaag atagcattaa aagtacataa
      481 agaaaaaaac attattgatg atacaaatct atctattata aaaaagacag aagcatatcc
      541 taattcaaaa actaaaatta gaggagtttc ttggaataaa gaaaaaagaa aatactgcgc
      601 acagatagaa tttaagaaaa cacattataa tttaggatac tatgacaaca taagagaagc
      661 agaagaagca tacaaaaaag ccaaagaaaa attcttaaaa gaaataaacg gaaaagttta
      721 aaaaaccatt gacttaataa acgcaaacgt ttataatata attaaagaaa gagataatca
      781 agaggaggat ataaaaaatg
//
LOCUS       DAFNNB010000315          796 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1046, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000315 DAFNNB010000000
VERSION     DAFNNB010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1046"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..149)
                     /locus_tag="L3M60_003587"
     CDS             complement(<1..149)
                     /locus_tag="L3M60_003587"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228189.1"
                     /translation="MAAQESAARIIRTVLHMLEPQFAVELWNGERIGDMDGPVLAIND
                     PMAVRR"
     gene            complement(504..>796)
                     /locus_tag="L3M60_003588"
     CDS             complement(504..>796)
                     /locus_tag="L3M60_003588"
                     /inference="COORDINATES: protein motif:HMM:TIGR02684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative addiction module antidote protein"
                     /protein_id="HBN6228190.1"
                     /translation="TMATKLYDYDPAEALKSDDAIEVFLADAFETGDAGHIAKALGVV
                     ARAKGMTSIARATGLAREQLYRSLSEDGNPTLETTIAVMKAIGFQITGKRAA"
BASE COUNT          162 a          243 c          206 g          185 t
ORIGIN      
        1 cggcgcacgg ccatcggatc gttgatcgca agcaccgggc cgtccatgtc gccgatacgc
       61 tcgccattcc atagctccac ggcaaattgc ggctccagca tgtgtaggac tgtgcggatg
      121 atccgcgccg cgctttcttg tgccgccatg caacaactcc aaatcatagg ctccggcaca
      181 ctgctgccgg aggtccggca gaacctatgc gcttcgtttc cggctggcaa cgcactttca
      241 cgggcagctg aaaacggcga ttgaatattt tcccttgaaa cctgacgcgg cagaccccat
      301 tctcaatttg tgttcaacga atgaaaagga ggtgatccga tgtctcgtga tttcgcaaac
      361 tgccacaggg cgtctgatgc tttccatcgg ggatcgtctc tccgttgaat aagcaccctg
      421 gctggtccag gggtaagaag gccaaacgac agctttgtgc cagacggaat tgcggggcca
      481 ctcttcggag tggccctttc cgttcaggca gcccgttttc ctgtaatctg gaaaccgatc
      541 gccttcatga cggcaatcgt cgtttcaagc gtcggattcc cgtcctcgct caaagacctg
      601 tacagttgtt cacgcgccaa gccggttgca cgggcaatgc tcgtcatccc tttggcgcgc
      661 gccacgacac ccagcgcttt tgcaatatga cctgcgtcgc ccgtttcaaa cgcatcggcc
      721 aggaacactt cgattgcatc gtccgatttc aaagcctctg ctggatcata atcataaagc
      781 tttgtcgcca tggtcg
//
LOCUS       DAFNNB010000316          792 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1056, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000316 DAFNNB010000000
VERSION     DAFNNB010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1056"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(210..362)
                     /locus_tag="L3M60_003589"
     CDS             complement(210..362)
                     /locus_tag="L3M60_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HBN6228191.1"
                     /translation="MATFIIAAIVVVLMALAVMYMIKNLKKNGSSCGCGCSGCSSSKS
                     CHSAKR"
     gene            complement(388..>792)
                     /locus_tag="L3M60_003590"
     CDS             complement(388..>792)
                     /locus_tag="L3M60_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HBN6228192.1"
                     /translation="VVGTFGVLFGISDATEQDPTLLASVASMFTVANAFAFMAFNMLC
                     APCFAAIGSIKREMGSWKWTWITLGFQTLTAYIVALLINQVGSLVLGTGGSIAGAIIS
                     VVIAVGVVFAVLTYSNRNMKKEKMGKLSYMKN"
BASE COUNT          281 a          161 c           73 g          277 t
ORIGIN      
        1 gtagctattt tttaatcaaa tttaaacata atatttactc atattgctta atttagcaat
       61 aatccctcct aatcatatat ttatttataa ttatacagta aaattacaac atagtctaac
      121 ttacttcaaa tatgaaattt gttatttata taaaaaataa gaacctgata ataaagtaat
      181 ctaaactttt tatcaggctc ttatcctatt tatctcttag cactatggca actcttacta
      241 cttgagcatc cagaacaacc acatccacaa ctgctaccat tcttttttaa atttttaatc
      301 atatacatta cagccaatgc cattaatact actacaatag ctgctattat aaatgtagcc
      361 atatttatca ctccttattt atatgaatta atttttcata taacttaatt tacccatttt
      421 ttcttttttc atatttctat ttgaataagt taaaactgca aataccactc ctactgctat
      481 aactacagat attattgccc cagcaatact tcctccagta cctaaaacta aacttccaac
      541 ttgattgata agaagagcaa ctatatatgc tgttaaagtc tgaaatccta atgtaatcca
      601 tgtccacttc catgaaccca tttctctttt tatagaccca atagctgcaa aacaaggtgc
      661 acaaagcata ttaaacgcca taaatgcaaa cgcattagct actgtaaaca tacttgcaac
      721 tgaagcaaga agtgttgggt cttgctctgt tgcatctgat attccaaata aaacaccaaa
      781 tgttccaact ac
//
LOCUS       DAFNNB010000317          767 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1111, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000317 DAFNNB010000000
VERSION     DAFNNB010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1111"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            194..637
                     /locus_tag="L3M60_003591"
     CDS             194..637
                     /locus_tag="L3M60_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009902048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBN6228193.1"
                     /translation="MIEIKTIEINKKGQIDLLMLADPCEEMIDKYLNKGTMYALYDNE
                     ELICIAVVNEISKEICELKNIATYEHFQNMGYASKMIYHLLDVYSKKYSSMIVGTSES
                     GVPFYEKFGFVYSHKIKNFFVDNYPEPIFEGELQCVDMLYLSYNF"
BASE COUNT          304 a           60 c          117 g          286 t
ORIGIN      
        1 attatagatg aatattaata ttgatattct aggtaattgt tagtgctgat attataattt
       61 attattagta ttaatattct agggaattat cactatattt taaaaaagta aaaatatata
      121 tcaaaatgaa ttaaataagt actgtaagaa gtttgctaaa ttaaggtttg aataaagtga
      181 gggtggagaa cttatgatag aaataaaaac tatagaaata aataaaaaag gtcaaattga
      241 ccttttaatg ttagccgacc catgtgagga aatgattgat aaatatctta ataaaggaac
      301 tatgtacgca ttgtatgata atgaagaact tatttgtata gcagttgtaa atgaaatttc
      361 caaagaaata tgtgaattaa aaaacattgc tacatatgag cattttcaaa atatgggtta
      421 tgcctcaaag atgatttatc atcttttaga tgtttatagt aaaaaatatt catcaatgat
      481 tgttggtaca tctgaaagcg gagttccttt ttatgagaag tttggatttg tttattcaca
      541 taaaattaaa aacttttttg tagataatta tccagaacca atctttgagg gagaattaca
      601 atgtgttgac atgttgtatc tatcatataa cttttagata ggataatatt tttaggttaa
      661 tataaataat ttattcatat aattattgga actataattt ataaattatg tataataaga
      721 gtataattta tttagaagct gttatattgt attagattca aataata
//
LOCUS       DAFNNB010000318          761 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1132, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000318 DAFNNB010000000
VERSION     DAFNNB010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1132"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..540)
                     /locus_tag="L3M60_003592"
     CDS             complement(<1..540)
                     /locus_tag="L3M60_003592"
                     /inference="COORDINATES: protein motif:HMM:NF012995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HBN6228194.1"
                     /translation="MTHASSSESGMILTSGQLDAYLTRIGVERPASLDVASLSKLHRA
                     HLMAFTWEALDAFMGWPSLIVPAAAFAKMVEGKRGGWCYEMNGLFGAALAALGFRVTR
                     LCGGVNRETLGDNAIGNHLTLRVDLDRPYLAEVGLADAIVEPVPLAVGPISQRGFDFS
                     IMPTDDGWLRFRNHARGMAH"
BASE COUNT          142 a          246 c          246 g          127 t
ORIGIN      
        1 gtgcgccatg ccgcgcgcat ggttcctgaa acgcagccag ccgtcgtccg tcggcatgat
       61 cgaaaaatcg aagccgcgct ggctgatcgg cccgacggca agcggtacag gctcgacgat
      121 ggcgtccgcc aagccgacct cggcaaggta cgggcggtcg agatcgacac gcagagtcag
      181 atggttgccg atggcgttgt cgcccagtgt ttcccgattg acgccgccgc acaggcgggt
      241 cacgcggaat ccgagcgcgg cgagcgccgc gccgaacaga ccattcatct cgtagcacca
      301 gccgccgcgc ttgccctcca ccatcttggc gaaggcggcg gccgggacaa ttaaggacgg
      361 ccatcccatg aaagcatcca gtgcctccca ggtgaaggcc atgagatggg cgcggtgaag
      421 tttcgacagg ctggcaacat cgagagaagc cggccgttcg accccgatcc ttgtaagata
      481 ggcgtccagt tggccggacg tcaggatcat tccactttcg cttgaagagg cgtgcgtcat
      541 gcttcagttt ccttcctatg gacgtgtaga gctctgtgtg aagacctcgc tgcgaagtcc
      601 tcgatttctg ccacgaggcg tgcgggcccc gggccttcct cggcgatggc atgcccaagt
      661 gccaacgccc gctcggcaaa gctcggctcg caaaggacgc gccgcacggc ctcgccgagc
      721 atttgcgcag gtgccgaagg gtcgaggcgc aggcccgcgc c
//
LOCUS       DAFNNB010000319          754 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1157, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000319 DAFNNB010000000
VERSION     DAFNNB010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1157"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            189..>754
                     /locus_tag="L3M60_003593"
     CDS             189..>754
                     /locus_tag="L3M60_003593"
                     /inference="COORDINATES: protein motif:HMM:NF015684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HBN6228195.1"
                     /translation="MIGWTLGRYFFLRYVTITCYFLMGSFALALILDFTELSSKLAAL
                     PEYSSIQAFGLSVMRIPYIMQQVFPFIALFSAMTTLISLNRKYELVVARSVGVSAWQF
                     LLPACTGAFLFGLLAVLIVNPLAAYGFEKSEAVQAAWRSGKTNDTTDARVPWLRQKTD
                     EGETIIGAKDVVNRGLRLVDASFIRLDK"
BASE COUNT          131 a          234 c          203 g          186 t
ORIGIN      
        1 ttcctctttg tttttccggt tcttgttacc ctgtcggcca tctacgcatt ggcgacgaat
       61 cgtcagatcg gcatgccggc aaagctggcc gacttcttca agaacgggtt ttcgtcacca
      121 caggcggccc ttgcaggtgt caggacaaag ctgctaaatc cccgctccgg acgtggagcg
      181 cgtgacgaat gataggctgg acactcggac gttacttctt tctccgctac gtgacgatca
      241 cctgctattt cctcatggga agttttgcgc tcgccctcat cctcgatttc accgagctgt
      301 cgagcaagct ggccgccctg ccggaatatt cctccattca ggcattcggc ctgtccgtga
      361 tgcgcattcc ctacatcatg cagcaggtgt ttccgttcat cgcgctgttt tcggcgatga
      421 ccacgctgat ctcgctcaac cggaagtacg agcttgtcgt ggctcgctcc gtcggtgtct
      481 cggcctggca gtttctcctg cccgcctgca ccggtgcgtt tttgttcggc cttctcgccg
      541 tcctgatcgt caatcccctc gccgcctatg gctttgagaa aagcgaggcc gtacaggcgg
      601 cctggcgcag cggaaaaacc aacgatacaa ccgatgctcg cgtgccgtgg ctgcgccaga
      661 aaaccgatga aggcgagacc atcatcggtg ccaaggatgt cgtcaaccgc gggttgcgtt
      721 tggtcgatgc ttccttcatc cggctggaca agaa
//
LOCUS       DAFNNB010000320          740 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1192, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000320 DAFNNB010000000
VERSION     DAFNNB010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1192"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
BASE COUNT          283 a           69 c           98 g          290 t
ORIGIN      
        1 atagaatagt atgtatttat ctaatgcggc aatataattt aaagctggtg ttgtagaaaa
       61 actaagagtg aatatagcaa agagaagcaa tagtatttta aactttgtct ctcttatttt
      121 ttgaaaacta acattaaaac ataaggtata aataatatta tcatataaat gaaaacaaat
      181 ttgaattttt ctctggtcga tgtatatagt aagtttgttg ataggagaaa aattattatt
      241 gataatatac atgcatagaa aggaaatggt atataatatt ttcttaagtt tcttttttag
      301 gcatttctat tggcatttta ggctatagta ctaaatacat taaccaacaa aataatgaaa
      361 taaatatata tatagaaaac ttaattttcg tgggaagaaa aaaagcaata atagcaagta
      421 ttattagaga ataacgaata atacgagtaa catttctttc tttttctatg atagttaaat
      481 cccagtgatt ttttataaat tcaaggtctt tttcttgact ctgattcatt taatcatttc
      541 ctgttaatag tataatttta aagctattac tttgttttat tttaacatat ttatacacta
      601 aagtgaattt tatgattaga tttatttaat tatttctgaa ttaaaactta ttagaattct
      661 ctattttgat aatttcaaac tctattgatt gtatgataat tgaaactata ggatatgtag
      721 gataacaagt ttcaaagtag
//
LOCUS       DAFNNB010000321          733 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1213, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000321 DAFNNB010000000
VERSION     DAFNNB010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1213"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>733)
                     /locus_tag="L3M60_003594"
     CDS             complement(<1..>733)
                     /locus_tag="L3M60_003594"
                     /inference="COORDINATES: protein motif:HMM:NF012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228196.1"
                     /translation="FEYKLLGIIPADRHFFGTLDGKPVHFLGTDKFGRDVLSRAIYGS
                     RVSLMIALIVVSIVTVVGTTVGLVSGYFGGRFDAWTQRFVELVLAFPQLPLYLALSSL
                     IPVTAPTSIFLGFVIIIMSALGWAQMSREVRGKTLALARIEYVRAALAVGASDRRIIF
                     QHIFPNVMSHVIVAVTIAIPHIVLLESFLGFLGFAVKPPLISWGLMLQDTATYSVIGS
                     YPWILTPVAFVLVTVFAFNALGDGLR"
BASE COUNT          196 a          199 c          233 g          105 t
ORIGIN      
        1 gcgcaaacca tcgcccaggg cattgaaggc aaagacggtg acaagcacga aggcgacggg
       61 ggtgagaatc cacgggtagg atccgatgac ggaataggtg gcggtgtcct gcagcatcag
      121 cccccaggaa atcagcggag gcttcaccgc aaaccccagg aagcctagaa aggattccag
      181 aaggacaatg tgcgggatcg cgatggtgac ggcaacgatc acatggctca tgacattggg
      241 aaagatgtgc tggaaaatga tgcgccggtc cgaagcgccc accgccagcg ccgcacgcac
      301 atattcgatg cgcgcaagtg ccagtgtctt gccgcgcact tcgcgcgaca tctgcgccca
      361 gccaagcgcc gacatgatga tgatgacgaa accaaggaaa atgctcgtcg gggccgtgac
      421 gggtatgagc gaactgaggg cgagatacag tggaagctgc ggaaatgcca ggaccagttc
      481 aacgaaacgc tgtgtccagg catcgaaccg tccgccgaaa tagccggaga ccagcccgac
      541 ggtcgtgccg acaaccgtca cgatggagac gacaatcaac gcaatcatca gcgagacgcg
      601 ggagccgtaa atggctctgg agagaacgtc acggccgaac ttgtcggttc caaggaaatg
      661 cacgggcttg ccgtcgaggg tgccgaagaa atgccggtcc gccggaatga tgccaagcag
      721 cttgtattca aag
//
LOCUS       DAFNNB010000322          710 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1280, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000322 DAFNNB010000000
VERSION     DAFNNB010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1280"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..500)
                     /locus_tag="L3M60_003595"
     CDS             complement(<1..500)
                     /locus_tag="L3M60_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228197.1"
                     /translation="MKAKNIGKYTICFIIVCFLMITMTLILSYFNKDKLSVKTLKGNV
                     KDIGRVSVVYTPNISKFSYDEVIISKNGVRNNTNPTSIIRNDYLNIDKKYSDFVNDKG
                     KDLIYKDDLNIGEAYTSVKYDLVQGMNALNDCIKVSNKNLKSGAEENFDINLNKSDFK
                     ENSWKT"
BASE COUNT          258 a          109 c           51 g          292 t
ORIGIN      
        1 aaagttttcc aactgttttc tttaaaatca gacttgttta aattaatatc aaaattttct
       61 tctgccccac tttttaaatt tttatttgaa acctttatac aatcatttaa agcattcatt
      121 ccttgtacta aatcgtactt aacagaagta tatgcttctc ctatattcaa atcatcttta
      181 tatataagat cttttccttt atcattaaca aaatcactgt attttttgtc aatatttaaa
      241 taatcatttc taattattga tgtgggattt gtattatttc ttactccatt tttacttata
      301 attacctcat cataagaaaa tttacttata ttaggtgtat atactacact cactctacct
      361 atatctttta cattgccttt taaagttttt acagataatt tatccttatt aaaataactc
      421 aatattaagg tcatagtaat cattaaaaaa cacactatta taaaacatat tgtatatttt
      481 cctatatttt ttgctttcat aatatccctc ctaaaagtca atcttttgct tcaaaagttt
      541 attagatata agactacata caaatatgtt tatacttgaa tatagaacag taaatacatt
      601 actgtattcg taaggtataa taaattctat agaaattata aacactaaaa tttccaaaac
      661 taaaaataga aaacttatca aagcgcttac tctataaata gatttattta
//
LOCUS       DAFNNB010000323          707 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1293, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000323 DAFNNB010000000
VERSION     DAFNNB010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1293"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            2..703
                     /gene="arsH"
                     /locus_tag="L3M60_003596"
     CDS             2..703
                     /gene="arsH"
                     /locus_tag="L3M60_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical resistance protein ArsH"
                     /protein_id="HBN6228198.1"
                     /translation="MPNVASSVLHVPDAERLSAPFSAHRPRILLLYGSLRKVSYSRFL
                     TQEAARLLEHFGAETRIFDPAGLPLPDDAPVDHPKVQELRDLSLWSEGQVWTSPERHG
                     AMTGVMKSQIDWIPLAMGAIRPTQGRTLAVMQVSGGSQSFNAVNQMRVLGRWMRMITI
                     PNQSSVAKAYQEFDENGRMKPSAYYDRVVDVMEELVKFTLLTRDISGYLTDRYSERKE
                     DAAALEKRVALKSAG"
BASE COUNT          140 a          208 c          214 g          145 t
ORIGIN      
        1 tttgcccaat gtcgcgtcga gcgtcctgca tgttccggac gctgaaaggc tgtctgcacc
       61 attctccgca caccgtccgc gcatccttct gctttacggc tccctccgaa aagtgtccta
      121 cagccgtttt ctgacccagg aggcagcgcg cctgctggag catttcgggg cggaaacccg
      181 cattttcgat ccggctggcc tgcctttgcc tgacgatgcg cctgttgatc atccgaaagt
      241 tcaggagctg cgcgacctct cgctgtggtc ggaggggcag gtatggacca gtcccgaacg
      301 gcacggtgcg atgaccgggg tgatgaaatc gcagatcgac tggatacccc tagccatggg
      361 cgcgatccgc cccacgcagg ggcggacgct ggcggtcatg caggtgtccg gcggatcgca
      421 atcattcaat gcggtcaatc agatgcgggt gctcggccgc tggatgcgca tgatcaccat
      481 cccgaaccag tcgtcagtcg ccaaggccta tcaggagttt gacgaaaacg gccgcatgaa
      541 accgtccgcc tattatgacc gcgttgtcga tgtgatggag gaattggtca aattcacgct
      601 gctgacgcgt gatatctccg gctatctcac cgatcgctac agtgagcgaa aggaagatgc
      661 ggcggcgctt gaaaagagag tcgcgctaaa gtcggccggt taacgca
//
LOCUS       DAFNNB010000324          697 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1328, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000324 DAFNNB010000000
VERSION     DAFNNB010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1328"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..678
                     /gene="nadC"
                     /locus_tag="L3M60_003597"
     CDS             <1..678
                     /gene="nadC"
                     /locus_tag="L3M60_003597"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBN6228199.1"
                     /translation="KRVFEILGNVGVELYKNDGDRVENREKIAVLTGSTRNLLVGERV
                     ALNYLQRMSGIATITSKYVEKLEGTNTKLLDSRKTIPNLRILDKYSVKVGGGCNHRFN
                     LSDGILLKDNHIGAAGGVKNAVELARKNTSFVRKIEVEVETLDMVKEAIEAKADIIML
                     DNMSLEMAKQAVDIINGRAIIEFSGNVNLDTIEDIGKIGVDVVSVGALTHSVKALDIS
                     MKNLRNI"
BASE COUNT          273 a           57 c          156 g          211 t
ORIGIN      
        1 aagagagttt ttgagatact tggtaatgta ggtgtagaat tatataaaaa tgatggtgat
       61 agagtagaga atcgtgaaaa gatagctgta ttaacaggta gtactagaaa tttacttgta
      121 ggagagcgtg tagcattgaa ttatttacag agaatgagtg gcatagctac tattacaagt
      181 aagtatgttg aaaagcttga aggtacaaat acaaaattat tagatagtag aaaaacgata
      241 cctaatctta gaatattaga taagtattct gttaaagtag gtgggggatg taatcacaga
      301 tttaatttat ctgatggaat attgcttaaa gataaccaca ttggggctgc tggtggtgtt
      361 aagaatgcag ttgaattagc tagaaaaaac acttcttttg ttagaaagat agaagtagaa
      421 gtagagactt tagacatggt aaaagaagca atagaagcta aggcagatat aataatgttg
      481 gataatatga gtttggaaat ggcaaaacaa gcagtagata ttataaatgg aagagcaatt
      541 atagaatttt ctggaaatgt aaatttagat accatagaag atataggaaa aatcggtgtg
      601 gatgttgttt ctgtaggagc acttacacat tctgttaaag ctctcgatat aagtatgaag
      661 aatttgagaa atatataaat tttatattga aataaat
//
LOCUS       DAFNNB010000325          657 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1484, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000325 DAFNNB010000000
VERSION     DAFNNB010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1484"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..505
                     /locus_tag="L3M60_003598"
     CDS             <1..505
                     /locus_tag="L3M60_003598"
                     /inference="COORDINATES: protein motif:HMM:NF012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228200.1"
                     /translation="VIYEYTFTPITGFYLLDAVLQGNWDAFWNIFSFIILPASLLGYF
                     SLAYISRMTRSFMLNELEQEYIVAARAKGLSETRIIWAHALRNAAVPLVTVIALSYAN
                     LLEGSVLTETVFSWPGLGLYITNSLQNADMNAVLGGTIIIGAVFIAINLLSDLMYQAL
                     DPRTRAR"
BASE COUNT          119 a          214 c          164 g          160 t
ORIGIN      
        1 cgtcatctat gaatacacat ttacacccat taccggcttt tatctgcttg acgcggtgct
       61 gcagggcaat tgggacgcct tctggaacat cttctcattc atcatcctgc ccgcttcgct
      121 gcttggttat ttctcgcttg cctatatcag ccgcatgacc cgcagtttca tgctcaatga
      181 gctggagcag gaatatatcg ttgccgcccg cgccaagggc ctgtcggaaa cccgcatcat
      241 ctgggcccat gccttgcgca acgccgccgt gccattggtg acggtcattg ccctgtccta
      301 tgccaatttg cttgaaggtt cagtcctgac cgaaaccgtc ttctcgtggc cgggcctcgg
      361 cctgtacatc accaattccc tgcaaaacgc ggatatgaac gcggtccttg gcggcaccat
      421 catcattggc gcagtgttca ttgccatcaa tctcctgtct gatcttatgt atcaggcgct
      481 tgacccacgg acgcgcgcac gatgatccag acaatccata caccgcctcc acccatgtca
      541 cgccgtcaat ggctgatgag tgaccgtccg caatcgcgct tgcaggcaag gctgggccgc
      601 gcctatctgg tgtggggccg cttcgccagc aaccggctgg cgcttgccgg ccttgcc
//
LOCUS       DAFNNB010000326          655 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1488, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000326 DAFNNB010000000
VERSION     DAFNNB010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1488"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>655)
                     /locus_tag="L3M60_003599"
     rRNA            complement(<1..>655)
                     /locus_tag="L3M60_003599"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          143 a          205 c          149 g          158 t
ORIGIN      
        1 agttggcttc gcgcttagat gctttcagcg cttatccatt ccgtacatag ctacccagcc
       61 atgcccttgg cagaacaact ggtacaccag aggtacgtcc atcccggtcc tctcgtacta
      121 aggacaggtc ttctcaaatt tcctacgcct gcgacggata gggaccgaac tgtctcacga
      181 cgttctgaac ccagctcgcg taccacttta atgggcgaac agcccaaccc ttgggaccta
      241 ctacagcccc aggatgtgat gagccgacat cgaggtgcca aacctccccg tcgatgtgga
      301 ctcttggggg agataagcct gttatcccca gggtagcttt tatccgttga gcgatggccc
      361 ttccacgcag aaccaccgga tcactaagtc cgattttcat ccttgctcga cctgtatgtc
      421 ttgcaatcaa gctctctttt gcctttacac tctacgtacg atttccgacc gtactgagag
      481 aacctttgag cgcctccgtt accttttggg aggcgaccgc cccagtcaaa ctgcccacct
      541 gacagtgtcc caagaccaga ttcatggcct atggttagag tcccagtact acaagggtgg
      601 tatcccaagg atggctccgc caaaactggc gtcctggttt caaagcctcc cacct
//
LOCUS       DAFNNB010000327          616 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1634, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000327 DAFNNB010000000
VERSION     DAFNNB010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1634"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>616
                     /locus_tag="L3M60_003600"
     CDS             <1..>616
                     /locus_tag="L3M60_003600"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012722.1,HMM:NF014567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HBN6228201.1"
                     /translation="PVERWYRATVLRLDQGGSDRQYLLSFRDLTEAHRLERMRSDFIA
                     NASHELRTPLASLSGFIETLRGPARNDAVARDNFLEIMQKQAERMARLIDDLLSLSRI
                     EMKAHIAVKDQVDLTIVLNHVVDTLRPLATHLDIEIERHIDPGPVMVLGDRDELTQVF
                     QNLLENACKYGQSGKKVVVELKNNGEGIKATIQDFGPGIPEEHIP"
BASE COUNT          129 a          187 c          178 g          122 t
ORIGIN      
        1 ccggttgaac gctggtatcg cgccacagtg ctgcgtctgg atcagggcgg ttccgaccgc
       61 cagtatctcc tgtcattccg cgatctgacc gaggcacatc gtttggagcg catgcgctcg
      121 gacttcatcg ccaatgccag ccatgaattg cgcacgccgc tggcgtcact cagcggtttc
      181 attgaaacgc tgcgcggtcc ggcgcgcaac gacgccgttg cccgcgacaa ttttctggag
      241 atcatgcaga agcaggccga gcgcatggca cggctcatcg atgatcttct gtcgctgtcg
      301 cgcatcgaaa tgaaggccca tatcgcggtc aaggaccagg tggacctgac catcgttctc
      361 aatcatgtgg tggatacgct ccgcccgctc gccacgcacc tcgacattga aatcgaacgc
      421 cacatcgatc ccgggccggt catggtcctc ggcgaccggg atgaactgac gcaggttttc
      481 cagaatcttc tggagaacgc ctgcaaatac ggccagtccg gcaagaaggt ggttgtcgag
      541 ctcaaaaaca atggcgaggg gatcaaagcc actattcagg attttggccc gggtataccg
      601 gaggagcaca tcccgc
//
LOCUS       DAFNNB010000328          614 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1650, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000328 DAFNNB010000000
VERSION     DAFNNB010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1650"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..228
                     /locus_tag="L3M60_003601"
     CDS             <1..228
                     /locus_tag="L3M60_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBN6228202.1"
                     /translation="LEIVLEKREAEALFASLTPRENEIINLVCRGWGSKEIATSLNIS
                     LRTVDAHRANISEKLGTTSVAEFVQMKMRVQ"
     gene            complement(250..>614)
                     /locus_tag="L3M60_003602"
     CDS             complement(250..>614)
                     /locus_tag="L3M60_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010967649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6228203.1"
                     /translation="KRITIHTIADHTVNIIQVSDNGPGLSPDTLAQLFQPFFTTKSSG
                     MGLGLSLSQRIVQRVGGLLTASNKDGAVFTITLPKAETRPATAPCIPLVAFDATNGMQ
                     REHDIKHVAAHTNKRYAG"
BASE COUNT          127 a          137 c          190 g          160 t
ORIGIN      
        1 ctcgagattg ttttggaaaa gcgagaggcc gaggcgttgt tcgcgtcatt gaccccgcgc
       61 gaaaatgaaa tcatcaatct cgtgtgccgg ggatggggtt cgaaggaaat tgcaacgtca
      121 ttgaacatct cattgcggac ggtcgatgct caccgggcca atatttccga gaaactgggc
      181 acgacatcgg tggctgaatt tgtgcagatg aaaatgaggg ttcagtaggg catcgaggcg
      241 ctttgttctt taaccggcat atcgcttgtt ggtgtgggcg gcgacatgct tgatgtcgtg
      301 ttcccgttgc attccgttgg ttgcgtcgaa agccactagc ggtatgcaag gtgctgttgc
      361 tggacgagtc tcggcttttg gcagggtaat cgtgaagacc gcgccatctt tgttcgaagc
      421 ggtgagcagc ccgccgacgc gttgcactat tctttgggac aacgagagac ccaatcccat
      481 tccgctgctc ttggttgtga agaagggctg aaagagctgt gcaagcgtat ccgggctcaa
      541 accgggccca ttgtctgaca cttggatgat attcacggtg tgatctgcga tggtgtggat
      601 ggttatacgc tttt
//
LOCUS       DAFNNB010000329          610 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1669, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000329 DAFNNB010000000
VERSION     DAFNNB010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..610
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1669"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(98..247)
                     /locus_tag="L3M60_003603"
     CDS             complement(98..247)
                     /locus_tag="L3M60_003603"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228204.1"
                     /translation="MNNLSVEKTITTVKRSLAYKKFFSFNITPATPNIKNIDAINFPI
                     RLLLG"
     gene            415..>610
                     /locus_tag="L3M60_003604"
     CDS             415..>610
                     /locus_tag="L3M60_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HBN6228205.1"
                     /translation="MGESKKSENLLLSIIYIIFFVYILMIIRIILFRDVPIYAISKGT
                     FRTVNLIPFYTIYQFIVDSNI"
BASE COUNT          209 a           50 c           90 g          261 t
ORIGIN      
        1 gtttcttttt taataaagga aaaatagcat ctaataaaat tgtgtttaat gcaataggag
       61 tacttacatt tgtactttgt tttatgcttt ttagttatta tcctaataat aatcttattg
      121 gaaaatttat tgcatctata ttttttatat ttggagttgc aggagttatg ttgaaagaaa
      181 aaaatttttt atatgctaga ctccttttaa ctgtagttat tgttttttct acactaagat
      241 tgttcattat tcaataaaat acatttgtat gaagttttgt taaagtctgc acatttaagt
      301 gtaggctttt ttgtattttt atttagaagt taagggaatt ttaagaaaat tttaaaatcg
      361 tcttaaatat ttattggtac acttgaaata ttaatagata gaggaggctt atcagtggga
      421 gaaagtaaaa agtcagagaa tttgttactg tcaattatat acataatatt ttttgtatat
      481 attttaatga ttataaggat aattttattt agagatgtac caatttatgc tatttccaag
      541 ggaacattta ggactgtaaa tttaattcca ttttatacaa tatatcaatt tatagtagat
      601 agtaatattg
//
LOCUS       DAFNNB010000330          602 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1705, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000330 DAFNNB010000000
VERSION     DAFNNB010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1705"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..153
                     /gene="tcdR"
                     /locus_tag="L3M60_003605"
     CDS             <1..153
                     /gene="tcdR"
                     /locus_tag="L3M60_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBN6228206.1"
                     /translation="QRKVIFYKYLKGYSDREISVKLKISRQAVNKAKNRAFKKIKKDY
                     ENYFNL"
BASE COUNT          268 a           44 c           88 g          202 t
ORIGIN      
        1 caaagaaaag ttatttttta taaatattta aaaggatatt ctgatagaga aatatcagtg
       61 aaattaaaaa tatctagaca agctgttaat aaggctaaaa atagagcatt taaaaaaata
      121 aaaaaagact atgaaaatta ttttaacttg taattaatga gtttaaagaa atatttacaa
      181 tagaaatcaa attttagaat taactttatt gtaaaatcaa taacttaatc taagaatatc
      241 ttaattttta tattttatat agaacaaagt ttacatattt atttcagaca acgtctttat
      301 tcaattgaag agcaaattaa tcaactgagt gtcttcgatt taaaatgtta ggaagtgaat
      361 gtatatgaaa acctaagtag atattagtat attttataaa tagaaaggag gatatataaa
      421 agagttttag catttagatg taaaaatatt atagtaaagg agaaaatttt atgagtttag
      481 ttaatagaaa acagttagaa aaaatggcaa atgtaagatt tcgtactcaa gaagatgaat
      541 acgttgcaat attggatgct ttagaagaat atcataatat gtcagagaat actgtagtcg
      601 aa
//
LOCUS       DAFNNB010000331          602 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1710, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000331 DAFNNB010000000
VERSION     DAFNNB010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1710"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(105..584)
                     /locus_tag="L3M60_003606"
     CDS             complement(105..584)
                     /locus_tag="L3M60_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HBN6228207.1"
                     /translation="MSNKIIKNLLSGILQILFFLLGLVIVVGGFKSFIYLCFSGEATL
                     QGTISGILMFILGVSYFIIIKLLIEVLGSSEYSLFVKENVKRFRVIGYLLLLNSLIEF
                     ISTFGTTGKGMRFLDLGFGFYFTVPVLVYFITSLMSFVIADGFVKAIKIKEDNDLTI"
BASE COUNT          240 a          101 c           44 g          217 t
ORIGIN      
        1 ttagcattat agtttatgca ttatacttta atatatttta ttttaaatta ctagttaacc
       61 tctcttttca agttatctat cttctatata ttaaagtata gttcctaaat agttaaatca
      121 ttatcctctt ttatctttat tgcctttaca aatccatctg caattacaaa actcataaga
      181 gaagttataa aatatacaag tactggaaca gtaaaataaa atccaaatcc taaatctagg
      241 aatctcattc cttttccagt agtaccaaaa gttgaaataa actcaattag agagtttaaa
      301 agcaaaagat atccaataac cctaaatctt tttacattct cttttacaaa taggctatat
      361 tcagaactac ctaatacttc tatcaataat tttataataa taaaatatga tacacctaaa
      421 ataaacatta atattccaga aatagtgcct tgtagagttg cttctccact aaaacatagg
      481 tatataaaac ttttaaatcc tcccacaact attactaatc ctaaaagaaa aaataaaatt
      541 tgtaatatac cacttaataa attttttata attttattag acatcatacc aactccttta
      601 aa
//
LOCUS       DAFNNB010000332          579 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1822, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000332 DAFNNB010000000
VERSION     DAFNNB010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..579
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1822"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>579
                     /locus_tag="L3M60_003607"
     CDS             <1..>579
                     /locus_tag="L3M60_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6228208.1"
                     /translation="FRKYIFGILVTLDISDIRDDTTINSEIKQKIEGIKDLSLEYESI
                     KDLTGINYFTGLEKLYCSSNELKKLDLSKNTKLEELNASSNKLKTINLNGSEKLKTLL
                     CNDNELSNLDVSKNTKLKYLYCDNNRLEKLDITNNKNLVGYSWGNQKEPEKPSEPEKP
                     SGGGSYTPAPIVTKINSLVGSDRYATSIKISKQ"
BASE COUNT          258 a           61 c           85 g          175 t
ORIGIN      
        1 tttagaaaat acatttttgg tattttagta actttggata tttcagatat tagagatgat
       61 actactataa atagcgaaat aaaacagaaa attgaaggaa taaaagactt aagtttagag
      121 tatgaatcta taaaagattt aacaggaatt aattatttta ctggattaga aaaattatat
      181 tgttcttcta atgaactaaa gaaattagat ttaagtaaaa atactaaatt agaagagtta
      241 aacgctagtt ctaataaatt aaaaacaata aatttaaatg gaagtgaaaa acttaaaact
      301 ttattgtgta acgataatga actaagcaat ctagatgtaa gtaaaaatac aaaacttaaa
      361 tatttatatt gtgataacaa tagactagaa aaattagata ttactaacaa taaaaatctt
      421 gtaggttact cttggggaaa ccagaaagag ccagaaaaac catctgagcc agagaaaccg
      481 tctggaggag gttcttatac tccagctcca atagttacaa aaataaattc tttagtaggt
      541 tctgatagat atgctacctc aataaaaata agtaaacaa
//
LOCUS       DAFNNB010000333          577 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1834, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000333 DAFNNB010000000
VERSION     DAFNNB010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..577
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1834"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>577
                     /locus_tag="L3M60_003608"
     rRNA            <1..>577
                     /locus_tag="L3M60_003608"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          154 a          121 c          190 g          112 t
ORIGIN      
        1 caggatggac ccgcgtctga ttagctagtt ggtaaggtaa cggcttacca aggcgacgat
       61 cagtagccga cctgagaggg tgatcggcca cattggaact gagacacggt ccaaactcct
      121 acgggaggca gcagtgggga atattgcaca atgggcgaaa gcctgatgca gcaacgccgc
      181 gtgagtgatg aaggccttcg ggtcgtaaaa ctctgtcctc aaggaagata atgacggtac
      241 ttgaggagga agccccggct aactacgtgc cagcagccgc ggtaatacgt agggggctag
      301 cgttatccgg atttactggg cgtaaagggt gcgtaggcgg tctttcaagt caggagtgaa
      361 aggctacggc tcaaccgtag taagctcttg aaactgggag acttgagtgc aggagaggag
      421 agtggaattc ctagtgtagc ggtgaaatgc gtagatatta ggaggaacac cagttgcgaa
      481 ggcggctctc tggactgtaa ctgacgctga ggcacgaaag cgtggggagc aaacaggatt
      541 agataccctg gtagtccacg ctgtaaacga tgagtac
//
LOCUS       DAFNNB010000334          566 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1902, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000334 DAFNNB010000000
VERSION     DAFNNB010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1902"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..370)
                     /gene="tcdR"
                     /locus_tag="L3M60_003609"
     CDS             complement(<1..370)
                     /gene="tcdR"
                     /locus_tag="L3M60_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003434528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylating toxin sigma factor TcdR"
                     /protein_id="HBN6228209.1"
                     /translation="MQKSFYELIVLARNNSVDDLQEILFMFKPLVKKLSRVLNYEEGE
                     TDLIIFFIELIKNIKLSSFSEKSDAIIVKYIHKSLLNKTFELSRRYSKTKFNFVEFDE
                     NILNMKNNYQSKSVFEEDICF"
BASE COUNT          229 a           72 c           38 g          227 t
ORIGIN      
        1 aaaaacaaat atcttcctca aaaacagact tactttgata attatttttc atatttaaga
       61 tattttcgtc aaattctaca aaattaaact tagtcttaga atatcttcta gataactcaa
      121 aagtcttatt caataatgat ttatgaatat atttaactat aatagcatca cttttttctg
      181 aaaagctact taatttaata ttttttatta attcaataaa aaatattatt aaatctgttt
      241 ctccctcttc ataatttaaa accctactaa gtttttttac taatggctta aacataaata
      301 aaatttcttg caaatcatct actgagttat ttcttgctaa aacaattaat tcgtaaaaag
      361 acttttgcat aaaatcatcc tctcttattt tataatgatg attttttaaa aaaaattgac
      421 aacatatgca ttaaatcagt cattataatt gtatgattag aaaataaatt aagttttata
      481 tgaaaatatt cattataaac acattaattt aataattaat tagataatct tatcctaata
      541 aataaagtat tttagcaaga aatttc
//
LOCUS       DAFNNB010000335          552 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.1974, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000335 DAFNNB010000000
VERSION     DAFNNB010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.1974"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(164..>552)
                     /locus_tag="L3M60_003610"
     CDS             complement(164..>552)
                     /locus_tag="L3M60_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228210.1"
                     /translation="KESCLIKETNNIKIYIDIQGKVEAIAVQTWAKLLDDNKEINIFT
                     LNQTPTHLNDMLGEICYVNDYEEFENWCENEWENLDWDSYKKFNKENFEEIAERNIDD
                     STSVFLEELQKGIESCKQELQNIVEN"
BASE COUNT          180 a           90 c           41 g          241 t
ORIGIN      
        1 ttatattatt tctctataaa ttaaattgac atttaaatcc ctcgtagtta atataaaaat
       61 tcttattaca atatcatttc taaataaatt aaaaaatatt ttaaatccct cgtagttaat
      121 cttaaaagcg atactatata cattaataat aatactcttc tacttaattt tcaactatat
      181 tttgcaattc ttgtttacaa ctttcaatgc ctttttgtaa ttcttctaaa aaaactgatg
      241 tgctatcgtc tatatttctt tcagcaattt cttcgaaatt ttctttattg aattttttat
      301 aactatccca atccaaattt tcccactcat tttcacacca attttcaaat tcttcataat
      361 cgtttacgta acaaatttct ccaagcatat cgtttaaatg agttggtgtt tgatttaatg
      421 tgaaaatatt aatttctttg ttatcatcta aaagtttagc ccaagtttga actgctatcg
      481 cttcaacttt tccttgtata tcgatataaa tttttatatt attagtctct ttgattaaac
      541 aactttcttt aa
//
LOCUS       DAFNNB010000336          544 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2019, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000336 DAFNNB010000000
VERSION     DAFNNB010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2019"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            248..>544
                     /locus_tag="L3M60_003611"
     CDS             248..>544
                     /locus_tag="L3M60_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6228211.1"
                     /translation="MMIRKIPQAELKVIKFIWRVDNTVTSKDVIEVMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDKYTNYAIIVKHKDYLSFETKDFLSNIHDDSLKSLIS"
BASE COUNT          242 a           52 c           76 g          174 t
ORIGIN      
        1 tccttgtgta aaaatatagg gatttttatt aaacaatatt aatgtggaaa taccattctg
       61 tactattata attaaatata taaaaataaa aatatttgaa caactatctt ttaaaatata
      121 acttttaata tttaaaaaat tagaaaatta atataaatac aaaaattcac attttttcat
      181 aaagtgtttg actaaaaaat tcacatattg tagaattaag gtagcaagag ttttgaggga
      241 ggtgtaaatg atgataagaa aaataccaca agcagaacta aaagtaataa aattcatttg
      301 gagagtagat aatacagtaa catcaaaaga tgttattgag gttatggaac aaaaatatgg
      361 ctggaaacaa actactacac tgacactttt atctaggctg gtaaaaagag ggttcttaga
      421 tgctcaaaaa atagataaat atacaaatta tgcaataata gtaaaacaca aagattattt
      481 aagttttgag acaaaggatt tcctaagtaa tatacatgat gattctttga aaagtttaat
      541 ttca
//
LOCUS       DAFNNB010000337          544 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2020, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000337 DAFNNB010000000
VERSION     DAFNNB010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2020"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            100..216
                     /gene="rrf"
                     /locus_tag="L3M60_003612"
     rRNA            100..216
                     /gene="rrf"
                     /locus_tag="L3M60_003612"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            223..297
                     /locus_tag="L3M60_003613"
     tRNA            223..297
                     /locus_tag="L3M60_003613"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:255..257,aa:Asn,seq:gtt)
     gene            302..376
                     /locus_tag="L3M60_003614"
     tRNA            302..376
                     /locus_tag="L3M60_003614"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:335..337,aa:Glu,seq:ttc)
     gene            382..457
                     /locus_tag="L3M60_003615"
     tRNA            382..457
                     /locus_tag="L3M60_003615"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:415..417,aa:Val,seq:tac)
     gene            463..539
                     /locus_tag="L3M60_003616"
     tRNA            463..539
                     /locus_tag="L3M60_003616"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:497..499,aa:Asp,seq:gtc)
BASE COUNT          152 a          120 c          140 g          132 t
ORIGIN      
        1 tgtagttttt agagtgttaa ctctaaaaaa ttaaaaaaag tattgacaaa aaaagtaaaa
       61 aatgttaata ttatacttgt cctagcaaca agacaaaaat gtggttataa tagcagagaa
      121 gatacacctg ttcccattcc gaacacagaa gttaagttct ctagcgctga tggtacttgg
      181 tgggaaactg cctgggagag taggacatag ccacgtaaat gttccaaggt agctcaacgg
      241 tggagcaacc ggctgttaac cggtaggctg tgggttcgag ccccaccctt ggagccatta
      301 aggctcgttg gtcaagaggt taagacaccg ccctttcacg gcggtaacag gggttcaatt
      361 cccctacgag tcaccatttg tgggatcata gctcagctgg gagagcacct gccttacaag
      421 cagggggtca caggttcgag ccctgttggt cccaccattt gcggcctggt agttcagttg
      481 gttagaatgc cagcctgtca cgctggaggt cgagggttcg aaccccttcc aggtcgccaa
      541 aata
//
LOCUS       DAFNNB010000338          543 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2022, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000338 DAFNNB010000000
VERSION     DAFNNB010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2022"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..512
                     /locus_tag="L3M60_003617"
     CDS             <1..512
                     /locus_tag="L3M60_003617"
                     /inference="COORDINATES: protein motif:HMM:NF019303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HBN6228212.1"
                     /translation="RAGIDLTRVLDRGAFDLTRALENDPHFLDHDHPDHACGPDCDHG
                     HDHAHHHHDHDHGHDHHHHHDHASPIHDTTVVSVSLRVGELNPDKFFPWVQKITQEQG
                     PNILRLKGILAFRNDHERYVIQGVHMIIEGDHQRAWKDNEKRESRLVFIGRELDHDAL
                     KSGLESCLA"
BASE COUNT          117 a          180 c          140 g          106 t
ORIGIN      
        1 agcgcgccgg cattgacctg acgcgggtgc ttgaccgcgg cgctttcgac ctgacgcgtg
       61 cattggaaaa cgatccgcat ttcctcgacc acgatcaccc cgatcatgcc tgcggccccg
      121 attgcgatca tggtcatgat cacgcgcatc atcatcacga tcatgaccat gggcatgacc
      181 accatcacca ccatgatcat gcatcgccga ttcatgacac cacggtcgtc tcggtctcct
      241 tgcgcgtcgg cgagctcaat cccgacaagt tcttcccgtg ggtgcagaag atcacccagg
      301 aacagggccc gaacatcctg cgcctcaagg gcattctcgc cttccggaac gatcacgagc
      361 gctatgtcat tcagggcgtc catatgatca tcgagggcga tcatcagcgc gcgtggaaag
      421 acaacgaaaa gcgcgaaagc cgccttgtct tcatcggccg tgaactcgac catgatgcgc
      481 tgaaatccgg acttgaaagc tgccttgcct gatgccgacc attgccccgc tagacctcgc
      541 cgg
//
LOCUS       DAFNNB010000339          543 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2023, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000339 DAFNNB010000000
VERSION     DAFNNB010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2023"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            117..446
                     /locus_tag="L3M60_003618"
     CDS             117..446
                     /locus_tag="L3M60_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009900171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228213.1"
                     /translation="MNQRIKELRQLKHLSQKKFGENLNLSQNHISSIEKGIRTVTDRT
                     INDICDKYHVNKKWLLTGEGEMFKDVLEGFNIDNKEVEEFVRLFYEVDDETREYVLGL
                     MQKTLKK"
BASE COUNT          215 a           51 c           86 g          191 t
ORIGIN      
        1 atatgtcaat agagtttttg agatattttt tattgtattt aatcatatta tatcgttgat
       61 ttgagatatt ttatatgata taatatctca aagaagtatc acaaggaggt gaaaaaatga
      121 atcaaagaat aaaagaatta agacaattaa aacatctttc tcaaaaaaaa tttggtgaaa
      181 atctaaattt atctcaaaat catatttctt ctatagaaaa aggtattcga actgtcactg
      241 atagaactat taatgatatt tgcgataaat atcatgtaaa taaaaaatgg ctattgactg
      301 gtgagggaga aatgttcaaa gatgtattag aaggttttaa tattgacaat aaagaagttg
      361 aagagtttgt aagacttttt tatgaagttg atgacgaaac tagagagtat gtattggggt
      421 tgatgcaaaa aaccctcaaa aaataaggca cattaatgtg ccttattttt tgaaattgaa
      481 tattccatgt aacctaaaat ttttaaaaaa atatcacgtt ctttttttct aatttcggta
      541 gta
//
LOCUS       DAFNNB010000340          520 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2172, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000340 DAFNNB010000000
VERSION     DAFNNB010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2172"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>520)
                     /locus_tag="L3M60_003619"
     CDS             complement(<1..>520)
                     /locus_tag="L3M60_003619"
                     /inference="COORDINATES: protein motif:HMM:NF019625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HBN6228214.1"
                     /translation="ASMGCAGEPPALDDEVTAQRAARLAKAAEQAAAASGFALPNYII
                     GTEVPVPGGADHELTEVTPTSPDAARHTIAVHRARFASEGLQAAFDRVIGIVVQPGVE
                     FGNENVVEYRPDQAVALKKLLAHEPQFVFEAHSTDYQTVQSLSALVRDGFPILKVGPG
                     LTFALREALYGL"
BASE COUNT           85 a          166 c          177 g           92 t
ORIGIN      
        1 tcgagcccgt agagtgcttc gcgcagtgca aaggtcaggc cggggccgac tttgagaatc
       61 ggaaacccgt cgcgcacgag cgccgaaagc gactgcacgg tctggtagtc ggtggaatgg
      121 gcttcgaaca cgaattgcgg ttcatgcgcc aagagtttct tcagcgccac ggcctgatcc
      181 ggcctgtatt cgacgacatt ctcattgccg aactcgacgc cgggctgcac gacaataccg
      241 atgacgcggt caaaggcagc ctgaagtcct tcgctggcaa agcgcgcgcg gtgcactgca
      301 atggtgtggc gtgccgcgtc cgggctggtg ggcgtgacct cggtgagttc atggtccgcg
      361 ccgcccggaa ccggtacttc cgtgccgatg atgtaattgg gcagagcaaa gccgcttgcc
      421 gccgccgcct gctcggcggc cttggcgagc cgcgcggcgc gctgggccgt cacctcgtca
      481 tcgagcgccg gcggctcccc ggcgcagccc atgctggcgt
//
LOCUS       DAFNNB010000341          519 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2177, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000341 DAFNNB010000000
VERSION     DAFNNB010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2177"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>519)
                     /locus_tag="L3M60_003620"
     CDS             complement(<1..>519)
                     /locus_tag="L3M60_003620"
                     /inference="COORDINATES: protein motif:HMM:NF012738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HBN6228215.1"
                     /translation="RAKPWLEFLSLVPFSLSGVVLALALIRLYSKLPLPLINTPNLLI
                     AAYVIITLPFMYRAVMNALESVDTNRLSEAAQSLGANPAQVLLRVIVPNIAPGIVNGS
                     LLVFSTAFAEFVLANLLTGTRFKTFPIYLVEFTRSDARQASALAFMSFAFAWLISLAI
                     LWSATRVRQNAR"
BASE COUNT          126 a          136 c          175 g           82 t
ORIGIN      
        1 ctctcgcgtt ctggcgaacc cgcgttgcac tccacaggat ggcaagtgaa atgagccagg
       61 cgaatgcaaa gctcatgaag gcaagggcgc ttgcctgccg ggcatcggaa cgcgtgaact
      121 cgacgagata gatcggaaag gtcttgaagc gcgtgccggt cagcagattg gcaaggacga
      181 actcggcgaa ggccgtggaa aagaccagca ggctgccgtt gacaatgcca ggtgcgatgt
      241 tgggcacgat aacccgcagc aaaacctgcg cggggttggc gccaaggctt tgggcagcct
      301 ctgaaagcct gttcgtatcc acgctttcaa gcgcgttcat caccgcgcgg tacatgaacg
      361 gcagcgtgat gatgacatag gccgcgatca gaagattggg cgtgttgatg agggggagcg
      421 gcagctttga atagaggcgg atgagagcaa gcgccagcac cacccccgac agggaaaagg
      481 gcacgagcga caggaattcc agccatggct tggcccgcg
//
LOCUS       DAFNNB010000342          507 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2255, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000342 DAFNNB010000000
VERSION     DAFNNB010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2255"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..471)
                     /locus_tag="L3M60_003621"
     CDS             complement(<1..471)
                     /locus_tag="L3M60_003621"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228216.1"
                     /translation="MIRLNTAALTKAMPQLLSLMLILFLNYLAFPGFFEIRLQDGRLF
                     GSLIDVLNRGAPVIILAIGMTAVIASRGVDLSVGAVMAVSGALAATMTVAGYPLPVVI
                     VAALGTGLACGLWNGILVAYFDLQPIVATLILMVAGRGIAQLITEGSIVTFTEPG"
BASE COUNT          126 a          145 c          161 g           75 t
ORIGIN      
        1 gccgggctcg gtgaaggtga caatcgaacc ctcggtgatc agctgcgcaa tgccgcggcc
       61 ggccaccatg aggatcaagg tcgccacgat gggttgcagg tcgaaatagg cgacgagaat
      121 gccgttccac aggccgcacg ccagacccgt gccgagggcg gcaacaatga cgacaggcag
      181 gggataaccg gcgacggtca tcgttgcagc cagcgcgccc gagacggcca tgacggcgcc
      241 gaccgaaaga tcgacaccgc gactggcgat gacagcggtc atgccgattg caaggatgat
      301 caccggggca ccgcgattga ggacatcgat caggctgccg aacagccggc cgtcctgaag
      361 gcgtatttcg aaaaatccgg gaaatgccag ataattgaga aacagtatga gcatcagcga
      421 aagcagctgc ggcatggctt tcgtcaacgc tgcagtattc agacggatca tgcaggggcc
      481 ccttcagatt cagcagcaat catctgc
//
LOCUS       DAFNNB010000343          499 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2322, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000343 DAFNNB010000000
VERSION     DAFNNB010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..499
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2322"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            225..>499
                     /locus_tag="L3M60_003622"
     CDS             225..>499
                     /locus_tag="L3M60_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HBN6228217.1"
                     /translation="MMIRKIPQAELKVMKFIWKVDTTVTSKDVIEAMEQKYGWKQTTT
                     LTLLSRLVKRGFLDAQKIDRYTHYTIIVKHKDYLSFETKDFLSNIHD"
BASE COUNT          222 a           48 c           76 g          153 t
ORIGIN      
        1 tgttaaatag ttttaatgtg taataacatt ttatactatt gtaattaaat atgtaaaaag
       61 gaaaatattt aaatagatat attttaaaat ctaactttta atattgtaaa aaaattagaa
      121 aattaacaaa aaatacaaaa attcacattt ttttcataaa gtgtttgact aaaaaattca
      181 catgttgtag aattaaggta gcaagagttt tgagggaggt gtaaatgatg ataagaaaaa
      241 taccacaagc agaactaaaa gtaatgaaat tcatttggaa agtagatact acagtgacat
      301 caaaagatgt tattgaagct atggaacaaa aatatggctg gaaacaaact actacactga
      361 cgcttttatc taggctggta aaaagagggt tcttagatgc tcaaaaaata gacagatata
      421 cacattatac aataatagta aaacataaag attatttaag ctttgagaca aaggatttcc
      481 taagtaatat acatgatga
//
LOCUS       DAFNNB010000344          462 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2560, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000344 DAFNNB010000000
VERSION     DAFNNB010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2560"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>462)
                     /locus_tag="L3M60_003623"
     CDS             complement(<1..>462)
                     /locus_tag="L3M60_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003430030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228218.1"
                     /translation="YIIENAVKSEQLTKIGNTIETVNAIGFSSFRNIMGYTESWFMFG
                     IIVCICYAFFIWYRDFNGRSKSIYTLIMIPKNRINIYISKLLNILFLVYSYTVVLTIS
                     LFITSKLLPRHMLGNVTSYGFVQETIYNLKMLLPYSFEILFVQYIFLLIGFV"
BASE COUNT          177 a           68 c           42 g          175 t
ORIGIN      
        1 tacaaatcct atcaataaaa atatatactg tacaaatagt atttcaaagc tatatggaag
       61 taacattttt agattatata ttgtttcttg tacaaaacca tagcttgtaa cattcccaag
      121 catatgtcta ggcaacaact tacttgtaat aaataaagat attgtaagta ctactgtata
      181 gctatacact agaaataata tatttaataa tttagatata taaatattaa ttctattttt
      241 aggtatcatt ataagtgtat atatagattt acttctccca ttaaagtctc tataccaaat
      301 aaaaaaagca tagcatatac atacaattat accaaacata aaccatgatt ctgtataacc
      361 cattatattt ctaaaacttg agaaacctat agcatttact gtttctatag tatttcctat
      421 ttttgttaac tgttcacttt taactgcatt ttctattatg ta
//
LOCUS       DAFNNB010000345          459 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2584, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000345 DAFNNB010000000
VERSION     DAFNNB010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2584"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            154..270
                     /gene="rrf"
                     /locus_tag="L3M60_003624"
     rRNA            154..270
                     /gene="rrf"
                     /locus_tag="L3M60_003624"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            276..364
                     /locus_tag="L3M60_003625"
     tRNA            276..364
                     /locus_tag="L3M60_003625"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:310..312,aa:Leu,seq:taa)
     gene            376..452
                     /locus_tag="L3M60_003626"
     tRNA            376..452
                     /locus_tag="L3M60_003626"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:410..412,aa:Ile,seq:cat)
BASE COUNT          144 a           86 c          104 g          125 t
ORIGIN      
        1 taactctata ttgttaaact tgtcaaaatt tatagatttt taatagtttt ataaggcaaa
       61 aaatcgaata aaaaaagtat tgacatatta agacaaagat gttattataa tacttgtctt
      121 agaaaaaaag ttaaatgaaa atttagacaa taatctggtt ataatagcag agaggataca
      181 cctgttccca ttccgaacac agaagttaag ctctctagcg ctgatggtac ttggtgggaa
      241 gctgcctggg agagtaggac atagccaggt gatgtgccga agtggcggaa ctggcagacg
      301 cacaggactt aaaatcctgc gggacttacc tctcgtaccg gttcgattcc ggtcttcggc
      361 accacaaatt attgtgggcc attagctcag ttggttagag cgcccggctc ataaccggta
      421 ggtctggggt tcgagtccct gatggcccac cacaataat
//
LOCUS       DAFNNB010000346          453 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2633, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000346 DAFNNB010000000
VERSION     DAFNNB010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2633"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
BASE COUNT          152 a           59 c           32 g          210 t
ORIGIN      
        1 cctagataat tatcttaatt atataataat acttttattc tacagttgca ataaaaaact
       61 cgtaataata acaaaaaaat ctattattgt agaatcttgt atattttttc tatttttata
      121 tatttttcag gatgattttt attataaaat tttttataaa catataattt ttttatactc
      181 atttgacttt cttttaattc tttagagtgt atttaagttt tctataaaaa gcatactctt
      241 ttagctgttt acttacttat ttgatagttt tgtttcttta attaaatatc tttcagaatc
      301 taagctagat gtctcaaaaa aaacagattt gtaatataat tattttttct gctattccta
      361 tttttccaac cttagtttca tagtaaagta cacttttcat atacactaca ttctttcata
      421 ctcattttca aatttaacta cacaaatagt ctt
//
LOCUS       DAFNNB010000347          445 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2716, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000347 DAFNNB010000000
VERSION     DAFNNB010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2716"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            44..382
                     /locus_tag="L3M60_003627"
     CDS             44..382
                     /locus_tag="L3M60_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009890712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HBN6228219.1"
                     /translation="MNLLVIGGHERMEKDYMVMAKKKGYKTKVYTTMSSKLNNSIGRP
                     DAVVILTSTVSHKMSRTVESQAKKQDILIVRHKNSSKVAFNECLDMVDECLGNCSKCK
                     LKDKQKQKNI"
BASE COUNT          197 a           44 c           76 g          128 t
ORIGIN      
        1 aataataatg aaaatcaatg tcaattaaat gggaggaaat ctaatgaact tattagtgat
       61 tggtggtcat gaaagaatgg aaaaggatta tatggttatg gccaagaaaa aaggttataa
      121 gacaaaggta tatactacta tgtcatctaa attaaataat tctataggaa gaccagatgc
      181 agttgtcata cttacatcaa ctgtttcaca taagatgtct agaacggttg agtctcaagc
      241 taaaaaacaa gatatattaa tcgtaaggca taaaaatagc agtaaagtag cctttaatga
      301 atgtttagat atggttgatg aatgtctggg aaattgttca aaatgtaagc ttaaagataa
      361 acaaaaacaa aaaaatatat aagtataaaa aatacataaa cataaaatta aaggtactga
      421 gataatagtt ataattatta tctca
//
LOCUS       DAFNNB010000348          445 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2720, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000348 DAFNNB010000000
VERSION     DAFNNB010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2720"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
BASE COUNT          170 a           49 c           51 g          175 t
ORIGIN      
        1 ttttgataat aatcatacaa atcccacaag ttttattaac gattttacac aatttattca
       61 gtattgttta tggacaattt caattagata tatttatgtt tggaacactt ttgactttat
      121 atacaataat tattattatg tattataaag aaaatcataa aaaaacaaga gattaatacc
      181 tcttattttt tatactatct taaagtttat ataaaaaact tttatattta ataaccctgg
      241 aatagggagt tttggcaatc caatataaaa ttgtatcaaa attaaggttt tgtgttataa
      301 cagtaaaaga aagagaacta tattctattt agtgacagaa ttgagttcgt aatttaaaat
      361 aaaaaattat ttaaattttt tgaacttgat ttttaaatcg aattcctagc cactcttgcc
      421 ggagtggctt tttaaatgcc taaat
//
LOCUS       DAFNNB010000349          428 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2846, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000349 DAFNNB010000000
VERSION     DAFNNB010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..428
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2846"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            51..332
                     /locus_tag="L3M60_003628"
     CDS             51..332
                     /locus_tag="L3M60_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009894338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228220.1"
                     /translation="MNVYLQIGKELSEKMTKSELNKFSQEVVNFLRYAGVDKDMKIKL
                     FQTIIEAVARKKITLPNDSKFTEGILQSSEFEMTENVGFILEGLSYRNN"
BASE COUNT          181 a           41 c           72 g          134 t
ORIGIN      
        1 attgtttaaa taatatgttt taactataat ctaaaaaggg gttggaaaaa atgaacgttt
       61 atctgcaaat cggaaaagaa ttatccgaaa aaatgactaa atctgaatta aataagtttt
      121 cacaagaagt tgtaaatttt ttgcgatatg ctggagttga taaggatatg aaaattaaac
      181 tattccaaac aatcatagaa gcggtggcaa ggaaaaaaat aacattacca aatgacagca
      241 aatttacgga aggaatattg caaagtagcg aatttgaaat gacggaaaat gtagggttta
      301 tacttgaagg attaagttat cgtaataact aaacattaaa aaaacagtgt atttattgaa
      361 tacactgttt ttatttccgt atagttaatc taaaattgga tattatatca tatagatata
      421 gattaaat
//
LOCUS       DAFNNB010000350          419 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.2953, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000350 DAFNNB010000000
VERSION     DAFNNB010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.2953"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..220
                     /locus_tag="L3M60_003629"
     CDS             <1..220
                     /locus_tag="L3M60_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003431035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HBN6228221.1"
                     /translation="TKSNDILLKKNTFLTTKEDKEFHGLGIKSIKNSLEKYGGAISIN
                     FSENLFCLNMMIPIRKSQKDSNIAYENS"
     gene            263..>419
                     /locus_tag="L3M60_003630"
     CDS             263..>419
                     /locus_tag="L3M60_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HBN6228222.1"
                     /translation="MIKSCADKIAFLFVLNQVIEKDDLEICSYGLEILLSSIINTLSS
                     LMIGLILG"
BASE COUNT          181 a           37 c           51 g          150 t
ORIGIN      
        1 tacaaaatca aatgatattt tattaaaaaa gaatactttt ttaactacaa aagaagataa
       61 agaatttcat ggattaggaa taaaaagtat aaaaaattct ttagaaaaat atggtggtgc
      121 aatttctata aatttctctg aaaatttatt ttgtttaaat atgatgatac caattagaaa
      181 atcacaaaaa gattcaaata ttgcatatga gaatagttaa tatccataat ttaccaataa
      241 aattatttaa ggagaaaact aaatgataaa atcttgtgct gataaaatag cttttttgtt
      301 tgttttaaat caagtaatag aaaaagatga tttagaaatc tgttcatatg ggttagaaat
      361 tttactatct tccataataa acactctatc ttcattaatg ataggtttaa tattaggta
//
LOCUS       DAFNNB010000351          413 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3001, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000351 DAFNNB010000000
VERSION     DAFNNB010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3001"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
BASE COUNT          130 a           54 c           31 g          198 t
ORIGIN      
        1 tttaaatttt aaatattttg tgtttattat ataatctgaa aaaaaagcag tcaatagcat
       61 ttttaatttt ttaaattttc aaaattatga tttttgaaaa atcgttttcc ttttatcata
      121 gcacctattt acgttaattc ttgtagtatt ttgtatttat tttgctaaat ttatagttaa
      181 ctttatttaa tttctattct acttatatag attcacctct tgctttttag ttttgaaatc
      241 gttttttctg aaaattcctt attatgattt atcaacttat ttttcttcat agtaattatt
      301 ttctaacttc caacttactt aaactttgca gttgctagta ttcacgttta catacttatc
      361 tgtcataatt aactaaaaat tcttaatata tctctcagta aaaacaattc aat
//
LOCUS       DAFNNB010000352          412 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3007, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000352 DAFNNB010000000
VERSION     DAFNNB010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3007"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>412
                     /locus_tag="L3M60_003631"
     CDS             <1..>412
                     /locus_tag="L3M60_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021394011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HBN6228223.1"
                     /translation="GWRIINNKKYYFNPNNAIAATHLYTINNDKYYFSYDGILQNGYI
                     TIERNNFYFDANNESKMVTGVFKGPNGFEYFAPANTHNNNIEGQAIVYQNKFLTLNGK
                     KYYFDNDSKAVTGWQTIDGKKYYFNLNTAVAVTGW"
BASE COUNT          155 a           53 c           66 g          138 t
ORIGIN      
        1 ggatggagaa ttattaacaa taagaaatat tactttaatc ctaataatgc tattgctgcg
       61 actcatctat acactataaa taacgacaag tattacttta gttatgatgg gattcttcaa
      121 aatggatata ttactattga aagaaataat ttctattttg atgctaataa tgaatctaaa
      181 atggtaacag gagtatttaa aggacctaat ggatttgagt attttgcacc tgctaatact
      241 cataataata acatagaagg tcaggctata gtttaccaga acaaattctt aactttgaat
      301 ggcaaaaaat attattttga taatgactca aaagcagtta ctggatggca aactattgat
      361 ggtaaaaaat attactttaa tcttaacact gctgttgcag ttactggatg gc
//
LOCUS       DAFNNB010000353          407 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3052, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000353 DAFNNB010000000
VERSION     DAFNNB010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3052"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>407
                     /locus_tag="L3M60_003632"
     CDS             <1..>407
                     /locus_tag="L3M60_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009903172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBN6228224.1"
                     /translation="YIDKFNLICDENNFLIEEIIFKSQLIYIKASKCIESSVIIKEIE
                     ALYIKIKKINDFNCKKMYFFIIGKIYFLFLDDALIAKVNFLKARKYADLLKDMQVSSL
                     CNIKIGECECSFENYECSMEYFNEVISNKKYRNV"
BASE COUNT          169 a           25 c           59 g          154 t
ORIGIN      
        1 tatatagata aatttaatct tatttgtgat gaaaataatt ttctaattga agaaattatt
       61 tttaaatcac aacttattta tattaaggca agtaagtgta tagaatcttc tgtaataatt
      121 aaagaaatag aagctttgta tataaaaata aaaaaaatta acgattttaa ctgtaaaaag
      181 atgtattttt ttattattgg taaaatatat tttttatttt tagatgatgc tctaattgct
      241 aaggtgaatt ttttaaaagc aagaaaatat gcagatttac taaaggatat gcaagtatct
      301 tcattatgta atataaagat aggcgagtgt gaatgttcat ttgaaaatta tgaatgttcg
      361 atggaatatt tcaatgaagt aataagcaat aaaaagtata gaaatgt
//
LOCUS       DAFNNB010000354          401 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3108, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000354 DAFNNB010000000
VERSION     DAFNNB010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3108"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(18..218)
                     /locus_tag="L3M60_003633"
     CDS             complement(18..218)
                     /locus_tag="L3M60_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003723180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBN6228225.1"
                     /translation="MNNGIVKWFNNEKGFGFISMESGDDVFAHFSAIQTSGFKSLEEG
                     QKVSFDIVKGARGPQAENITIL"
BASE COUNT          143 a           61 c           31 g          166 t
ORIGIN      
        1 tccgaaaaat ctatatatta taatatagtt atattttctg cttgaggacc tctagcgcct
       61 ttaactatat caaagcttac tttttgacct tcttctaatg atttaaatcc tgaagtttgt
      121 atagctgaga aatgagcaaa tacatcatct ccactttcca ttgatataaa tccaaatcct
      181 ttttcattat taaaccattt tactattcca ttattcatta aaaatactcc tattaaccta
      241 tatcaagata tacttgattt acttgtgtat taccacaaat actattgtat catagattaa
      301 tagaaatgtc tatttaatta taatttttag ttaacatttt tatttattct taatatgaaa
      361 ataaatttaa acattttata ttaccaatat attaatgtaa a
//
LOCUS       DAFNNB010000355          387 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3232, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000355 DAFNNB010000000
VERSION     DAFNNB010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3232"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..164
                     /locus_tag="L3M60_003634"
     CDS             <1..164
                     /locus_tag="L3M60_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009904940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228226.1"
                     /translation="NVSVLVKAGAVSETTATDSVANATFEVKKHVAAPISTIGKISSG
                     LSSLFNILR"
BASE COUNT          159 a           47 c           48 g          133 t
ORIGIN      
        1 ataatgtaag tgtgttagtt aaagcaggag cagtatctga aacaactgct acagattcag
       61 tagcaaatgc aacatttgaa gttaaaaaac atgtggcagc tccaatatca acaataggaa
      121 aaatatcttc tggactatca agtttattta acatcttgag ataaataatt aagcataatt
      181 aaaaactagt ttcaaaaaaa gtaaatcttc ttgtatatac taatattagc tgtaggattt
      241 ttaaaataat tttcctacag ctttttaata tccaagagta gcaaattttt taaataaaat
      301 ctgctactct atagtatttt aaaacttaga gtattgaaat atttatgtgt attatttaaa
      361 taaaaattta tttaaaaacc caaaact
//
LOCUS       DAFNNB010000356          385 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3265, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000356 DAFNNB010000000
VERSION     DAFNNB010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3265"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..333)
                     /locus_tag="L3M60_003635"
     CDS             complement(<1..333)
                     /locus_tag="L3M60_003635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228227.1"
                     /translation="MAITLTALLFLIIAAALGAGGVWLAVLGGSWYYVIAALAFLVTS
                     ILLFRRRSAALPVYALLVIGTLGWAIWEIRFDWWQLAPRGGVIILLGLWLLTPWVRGR
                     LTRADAGSA"
BASE COUNT           94 a          120 c          128 g           43 t
ORIGIN      
        1 ggcagagccg gcatcggcac gggttagccg gccgcgcacc cacggtgtca ggagccagag
       61 cccgagcagg atgatcacgc cgccgcgcgg cgccagttgc caccaatcga agcgaatttc
      121 ccagatggcc cagcccagcg tgccgatcac cagcagcgca tagaccggca atgcggccga
      181 gcgccgccgg aagaggagaa tggatgtgac gagaaaggcg agcgcagcga tgacgtagta
      241 ccagctgccg ccgagaacgg cgagccagac gccgcccgca cccagggcag ccgcgatgat
      301 cagaaaaagg agagccgtca gagtgattgc catggcaggg atcctttcca agctcaatca
      361 agatcgaatg gtcctgacat gaggc
//
LOCUS       DAFNNB010000357          375 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3384, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000357 DAFNNB010000000
VERSION     DAFNNB010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3384"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>375
                     /locus_tag="L3M60_003636"
     CDS             <1..>375
                     /locus_tag="L3M60_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228228.1"
                     /translation="VLALAFVFICKVLLDLFLKTGLGYTLKGVGDNSQMIKSLGINIG
                     SIKILGLMISNGLIALSGSLMAQFLGFSDVNMGIGTLVLGIASIIIGITLFKKFTFIK
                     DTTAIIVGSFIYQFTIYFAMSLG"
BASE COUNT          112 a           54 c           62 g          147 t
ORIGIN      
        1 gttttagcct tagcttttgt attcatctgt aaagtccttt tagacttatt cctaaaaaca
       61 ggtcttggat acactttaaa gggtgttggt gataactctc aaatgattaa gtctctaggt
      121 ataaatatcg gttctataaa aatactagga cttatgattt caaatggact tatagcttta
      181 tctggtagtc ttatggctca gttcttagga ttttcagatg taaatatggg tataggaact
      241 ttagttcttg gaattgcctc tatcataatt ggtattacac tatttaagaa attcactttt
      301 atcaaagata ctactgcaat aattgttggc tcttttatat accaattcac tatatacttt
      361 gcaatgagtt tagga
//
LOCUS       DAFNNB010000358          374 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3401, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000358 DAFNNB010000000
VERSION     DAFNNB010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3401"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            200..307
                     /locus_tag="L3M60_003637"
     CDS             200..307
                     /locus_tag="L3M60_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013483530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBN6228229.1"
                     /translation="MRRHLWNPSYFIATVSENTEEQIRKYINNQKKQVK"
     gene            309..>374
                     /locus_tag="L3M60_003638"
     CDS             309..>374
                     /locus_tag="L3M60_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6228230.1"
                     /translation="MVVVKKAYKFRMYPNKKQQELI"
BASE COUNT          161 a           37 c           63 g          113 t
ORIGIN      
        1 tatgtgaacg acaacctcaa aaatgataaa agttaagact tacagttctt atatatgtta
       61 tttaaaaata aatataaaac cttcaaaata cttgttttaa gtaattgcgt attttaatat
      121 tatgataggt gatgaaaaaa ggaagttact catggtaggg gatatgcata tagtatgtca
      181 aatataaaca tccagtatta tgaggagaca tctttggaat cctagttatt tcattgctac
      241 tgtatcagaa aatacagagg agcagataag aaaatatatt aataatcaga aaaaacaagt
      301 aaagtgaggt ggtagtagtg aaaaaagctt ataagtttag aatgtatcca aataaaaaac
      361 aacaagagtt aatt
//
LOCUS       DAFNNB010000359          365 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3495, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000359 DAFNNB010000000
VERSION     DAFNNB010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3495"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(86..161)
                     /locus_tag="L3M60_003639"
     tRNA            complement(86..161)
                     /locus_tag="L3M60_003639"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(126..128),aa:Gln,seq:ttg)
     gene            complement(169..245)
                     /locus_tag="L3M60_003640"
     tRNA            complement(169..245)
                     /locus_tag="L3M60_003640"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(209..211),aa:Arg,seq:tct)
     gene            complement(274..357)
                     /locus_tag="L3M60_003641"
     tRNA            complement(274..357)
                     /locus_tag="L3M60_003641"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(321..323),aa:Leu,seq:tag)
BASE COUNT          111 a           87 c           73 g           94 t
ORIGIN      
        1 ttcggatact taaacttatt ctaaaattga atctaactac cttaaaataa aaatagctca
       61 aatatagttt agtaaataca tcatttggct ggggatgcag gactcgaacc tacgcataca
      121 tgagtcaaag tcatgtgcct taccgacttg gctaatcccc aatatatgtg gtgagcgcac
      181 agggattcga accccggaca cacgccttag aagggcgttg ctctatccag ctgagctatg
      241 cacccacaat tatatatgta tatcaaataa aaatggtgcg gatgaaggga gtcgaacccc
      301 cacgccaatg gcgctagatc ctaagtctag tgcgtctgcc aattccgcca catccgcgca
      361 ttttt
//
LOCUS       DAFNNB010000360          339 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3786, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000360 DAFNNB010000000
VERSION     DAFNNB010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3786"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>339
                     /locus_tag="L3M60_003642"
     CDS             <1..>339
                     /locus_tag="L3M60_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HBN6228231.1"
                     /translation="FNFKYLIIAVIFLISVTMLILISHINILIMADINNKDICLKLNI
                     KYMFNLININRQLYPAENSKNNDKKEGMKNNIDSSILLADDLLSVYRLLKKIKIHELY
                     SNINFGTENIG"
BASE COUNT          146 a           28 c           42 g          123 t
ORIGIN      
        1 tttaatttta agtatttaat aatagctgta atttttctta taagtgtaac tatgttgatt
       61 ttaatatctc atataaacat cttaataatg gctgatataa acaacaaaga tatttgctta
      121 aagcttaata taaaatacat gtttaattta ataaatataa atagacaact ttatccagct
      181 gaaaatagta aaaataatga taaaaaagag gggatgaaga ataatataga ttcaagtata
      241 ttattagctg atgacctttt gagcgtttat agattattaa aaaagataaa aatacatgaa
      301 ctttattcaa atattaattt tggaactgaa aatattggc
//
LOCUS       DAFNNB010000361          332 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3873, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000361 DAFNNB010000000
VERSION     DAFNNB010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3873"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            43..>332
                     /locus_tag="L3M60_003643"
     CDS             43..>332
                     /locus_tag="L3M60_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine kinase"
                     /protein_id="HBN6228232.1"
                     /translation="MQKKVAAINDLSGIGKCSLSVAIPILSALKVQCCPFPTAILSSQ
                     TGYPEFTFLDFTDEMVKYSNVWKNLKVHFDSIYSGFLGSKHQIEIVANFIND"
BASE COUNT          114 a           44 c           53 g          121 t
ORIGIN      
        1 ttggtatcat tataattaat acatactaat ggagggtttt ttatgcaaaa aaaagtcgca
       61 gcaattaatg acttatctgg aataggaaaa tgctcattat cagttgctat acctatactt
      121 tcagcattaa aggttcaatg ttgtccattt ccaacagcca tactttcaag tcaaactggt
      181 tatcctgaat ttactttttt agactttact gatgaaatgg taaaatattc aaatgtttgg
      241 aaaaatttaa aagtccactt tgatagtata tacagtggtt ttttgggttc aaagcatcaa
      301 attgagatag tggctaattt tataaatgat ta
//
LOCUS       DAFNNB010000362          324 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.3986, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000362 DAFNNB010000000
VERSION     DAFNNB010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.3986"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>324)
                     /locus_tag="L3M60_003644"
     CDS             complement(<1..>324)
                     /locus_tag="L3M60_003644"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228233.1"
                     /translation="TLKGIAIDDQGIIPEALDAACKESSIRALFVQPAAINPYATVMG
                     SVRRAQIVEVARKRDIAIVESDILGPLVEDRPPPIAALAPERTLYFTSFTKTVVPGLR
                     IGYLAA"
BASE COUNT           64 a           94 c          107 g           59 t
ORIGIN      
        1 gccgcgagat agccgatgcg caaaccgggg acgacagtct tggtaaagct ggtgaaatag
       61 agggtccgct ctggagccag tgcggcaatc ggcggcgggc gatcctcgac cagggggccg
      121 agtatgtcgc tttcgacgat ggcaatatcg cgcttgcgtg caacctcgac aatctgggcg
      181 cggcggaccg atcccatcac cgtcgcatac ggattgatcg ccgccggctg cacaaatagc
      241 gcgcgaatgc tggattcctt gcacgcggcg tccaacgcct cggggatgat accctgatcg
      301 tcaatggcga tgcctttgag cgtg
//
LOCUS       DAFNNB010000363          322 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.4012, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000363 DAFNNB010000000
VERSION     DAFNNB010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.4012"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..320)
                     /locus_tag="L3M60_003645"
     CDS             complement(<1..320)
                     /locus_tag="L3M60_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228234.1"
                     /translation="MSVMTQSLILSIMALGVYITYKILDFPDMSADGSYTMGASIVAF
                     SLTNGISPVVATLMAILCGCTAGLVTGILHIKFKISNLLSGILVMGMLYSINLRIMGK
                     SNIPL"
BASE COUNT          125 a           58 c           52 g           87 t
ORIGIN      
        1 agaggaatat ttgattttcc cattattctt aagttgatag agtataacat tcccatgact
       61 aaaataccag aaagtaagtt agatatctta aacttaatgt gaagaatccc tgtaacaaga
      121 ccagcagtac aaccacataa gatagccatt aaagttgcta ccacaggaga aataccatta
      181 gtaagagaaa atgctacgat agaagcaccc attgtgtagc taccatcagc agacatatct
      241 gggaaatcta ggattttgta agttatataa acccccagtg ccataatcga taaaattaaa
      301 ctttgcgtca ttacagatat aa
//
LOCUS       DAFNNB010000364          287 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.4489, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000364 DAFNNB010000000
VERSION     DAFNNB010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.4489"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            151..>287
                     /locus_tag="L3M60_003646"
     CDS             151..>287
                     /locus_tag="L3M60_003646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228235.1"
                     /translation="MSRVTTIDELEVIYGNGAIAEASTAKVADRIIPEYRQLIEGSPF
                     VA"
BASE COUNT           75 a           73 c           76 g           63 t
ORIGIN      
        1 gcccatgaaa acctcctcaa catgcatccg gtgcgactga tatacgagtg cgatgccgct
       61 gtcatttgtt aaaaataccg gctgtcatca agacttaaac atgcgatgtt agacggcaaa
      121 cgcatttgca cgacacagaa ggaggttgcg ttgtcccgcg ttacgacaat tgatgaactt
      181 gaggtcattt acggcaatgg cgcgatagcg gaagcatcga ccgccaaggt ggcggaccgc
      241 atcattccgg aataccggca actgatcgaa ggttcgccct ttgtggc
//
LOCUS       DAFNNB010000365          275 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.4710, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000365 DAFNNB010000000
VERSION     DAFNNB010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.4710"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
BASE COUNT           68 a           74 c           84 g           49 t
ORIGIN      
        1 gagcccggcc atgataatca gcgagccgga tagggatcga agcaaagtca taactggctt
       61 ccttgcaaag aagacacgga tgattaggaa taccagcgat caccgccaga gaacagatgg
      121 agtgcccttt ggaaacggca atggatgtaa cagcgttaac ggcctttctc gagcgggagt
      181 tcccacaggt caatgccgac ggggttgcct acaccgttac cggtgccggc ccgggttatg
      241 ccacgatgcg gcttgatccg gcggaccggc acctg
//
LOCUS       DAFNNB010000366          267 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.4856, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000366 DAFNNB010000000
VERSION     DAFNNB010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.4856"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            151..267
                     /gene="rrf"
                     /locus_tag="L3M60_003647"
     rRNA            151..267
                     /gene="rrf"
                     /locus_tag="L3M60_003647"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT           93 a           38 c           50 g           86 t
ORIGIN      
        1 tgcagctttt agagtgttaa ctctatgtaa ggtactaact tcaattaatg tagtatatat
       61 cataaaaaca attattattt agtactttaa aactattgac atttaaaaat aaagatgtta
      121 atataatact tgtcttataa ataggcaata tgtggttata atagcaaaga ggatacacct
      181 gttcccattc cgaacacaga agttaagctc tttagcgctg atggtacttg gtgggaaact
      241 gcctgggaga gtaggacgta gccacgt
//
LOCUS       DAFNNB010000367          259 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.4995, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000367 DAFNNB010000000
VERSION     DAFNNB010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.4995"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>259
                     /gene="tuf"
                     /locus_tag="L3M60_003648"
     CDS             <1..>259
                     /gene="tuf"
                     /locus_tag="L3M60_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005791980.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBN6228236.1"
                     /translation="TKFTAEVYVLKKEEGGRHTPFFDGYRPQFYFRTTDVTGACKLPE
                     GIEMVMPGDNVTMEVDLINSIVVEEGLRFSIREGGRTVASGV"
BASE COUNT          105 a           33 c           61 g           60 t
ORIGIN      
        1 acaaagttta cagcagaagt atatgtactt aaaaaagaag aaggtggaag acatacacca
       61 ttctttgatg gatatagacc acaattttac ttcagaacaa cagacgtaac aggagcttgt
      121 aagttaccag aaggaataga gatggtaatg cctggagata acgtaacaat ggaagtagac
      181 ttaataaact caatagttgt agaagaggga ttaagattct caataagaga aggtggaaga
      241 acagtagctt caggagttg
//
LOCUS       DAFNNB010000368          250 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.5179, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000368 DAFNNB010000000
VERSION     DAFNNB010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..250
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.5179"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..228
                     /locus_tag="L3M60_003649"
     CDS             <1..228
                     /locus_tag="L3M60_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HBN6228237.1"
                     /translation="VNGMGGTPQIELYIVYRKAYEIAAKHGLKVVRNLVGSYVTSLEM
                     AGCSITLLKLDDEMVKFWDAPVVTPSLRWGA"
BASE COUNT           51 a           65 c           82 g           52 t
ORIGIN      
        1 gtcaatggca tggggggaac gccgcagatc gaactgtata tcgtctatcg caaggcatat
       61 gagatcgcgg cgaagcacgg gctgaaagtc gtccgcaatc ttgtcgggtc ctatgtcacc
      121 agtctggaaa tggcgggctg ctcgatcacc ctgctgaagc tggacgatga aatggtgaag
      181 ttctgggacg ctccggtggt cacgccatcg ctgcgctggg gcgcctgaca tgatcggctg
      241 aagccctctg
//
LOCUS       DAFNNB010000369          249 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.5198, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000369 DAFNNB010000000
VERSION     DAFNNB010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..249
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.5198"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..>249)
                     /locus_tag="L3M60_003650"
     rRNA            complement(<1..>249)
                     /locus_tag="L3M60_003650"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           57 a           72 c           56 g           64 t
ORIGIN      
        1 gatccagccg caccttccga tacggctacc ttgttacgac ttcaccccag ttattgattc
       61 caccttcgac gacttcttcc aaaaggttag ataatcggct tcgggcgtct ccaactcccg
      121 tggtgtgacg ggcggtgtgt acaagacccg ggaacgcatt caccgcagca ttctgatctg
      181 cgattactag taactccagc ttcatgtagg cgagtttcag cctacaatcc gaactgagag
      241 tagctttaa
//
LOCUS       DAFNNB010000370          248 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.5216, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000370 DAFNNB010000000
VERSION     DAFNNB010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.5216"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>248
                     /locus_tag="L3M60_003651"
     CDS             <1..>248
                     /locus_tag="L3M60_003651"
                     /inference="COORDINATES: protein motif:HMM:NF014310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228238.1"
                     /translation="LKQLGAACDAETLFLALSDAWQENSRIWNEGRGLADIRKRWLKR
                     AAGIGSEVAVRVDGSVLRGIFETIDEDCRFVIRDNDGER"
BASE COUNT           51 a           61 c           87 g           49 t
ORIGIN      
        1 ctgaagcagc ttggtgcggc ctgcgatgcg gaaacgctgt ttctggcgct atccgatgcc
       61 tggcaggaaa acagccgcat ctggaacgag gggcgcgggc ttgccgacat tcgcaagcgc
      121 tggctcaagc gcgcggcagg aattggcagt gaagttgccg tgcgggtcga cggctcggtg
      181 ctgcgtggta tattcgaaac aattgacgaa gactgccgtt tcgtcatacg cgataatgat
      241 ggggaacg
//
LOCUS       DAFNNB010000371          236 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.5447, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000371 DAFNNB010000000
VERSION     DAFNNB010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.5447"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
BASE COUNT           77 a           58 c           54 g           47 t
ORIGIN      
        1 cgtcgccggg tacttacagc gatacgatca ttgtgacggt cacgtattag atcgcaacca
       61 tcgcagtatt tgatgggatc gcggaaaaga aaaagagttc tgaaaaagca aaaggccggg
      121 agctacccga ccttcctaaa cttgcctcaa caccagtgcc agtacatccg tggtcaaaaa
      181 tggaaacaag ctattcgcta cacgagaagc attaccctga cattgcgaca gaataa
//
LOCUS       DAFNNB010000372          223 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.5760, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000372 DAFNNB010000000
VERSION     DAFNNB010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.5760"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>223
                     /locus_tag="L3M60_003652"
     CDS             <1..>223
                     /locus_tag="L3M60_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009893147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cell wall-binding repeat-containing protein"
                     /protein_id="HBN6228239.1"
                     /translation="VNGEKGLADAVSMGAISAKEKMPILLTNQNDDMKEIEELIDNKD
                     ISKSYIVGGESLFNKDIEDKLPSVIKISG"
BASE COUNT           99 a           21 c           42 g           61 t
ORIGIN      
        1 tagtaaatgg agaaaaaggt cttgctgatg cagtaagtat gggagctata tcagcaaaag
       61 aaaaaatgcc aattcttctt actaatcaaa atgatgatat gaaggaaata gaagaactaa
      121 ttgataataa agatatatca aaatcatata tagttggtgg agagagttta ttcaataaag
      181 atatagaaga taaacttcca tctgtaatta aaatatcagg aga
//
LOCUS       DAFNNB010000373          218 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.5852, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000373 DAFNNB010000000
VERSION     DAFNNB010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..218
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.5852"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>218
                     /locus_tag="L3M60_003653"
     CDS             <1..>218
                     /locus_tag="L3M60_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228240.1"
                     /translation="KCMLGAKLKDLGYGTGVYKEPKLVSVKVPVFSMSKLSKVEVSLG
                     PEMKSTGEVLGVGENLEEALYKGFLAAG"
BASE COUNT           85 a           23 c           52 g           58 t
ORIGIN      
        1 aagtgtatgt taggagcaaa attaaaagat ttaggatatg gtacaggagt ttacaaagag
       61 ccaaaattag tgtcagtaaa agtaccagta ttctcaatgt ctaaattatc taaagttgaa
      121 gtaagtctag gacctgaaat gaagtctaca ggagaagtac taggtgtagg agaaaattta
      181 gaagaagcct tatataaagg attcttggca gcaggtag
//
LOCUS       DAFNNB010000374          206 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.6164, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000374 DAFNNB010000000
VERSION     DAFNNB010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..206
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.6164"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..167)
                     /locus_tag="L3M60_003654"
     rRNA            complement(<1..167)
                     /locus_tag="L3M60_003654"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           52 a           55 c           39 g           60 t
ORIGIN      
        1 ttatcccgta ttagcatacc tttcggtatg ttatccgtgt gtacagggta ggttacccac
       61 gcgttactca cccgtccgcc gctctttacc gaagtaaatc gctcaacttg catgtgttag
      121 gcacgccgcc agcgttcatc ctgagccagg atcaaactct caaataaaag tttatcaggc
      181 tcagatacta ttgttatcaa aatatc
//
LOCUS       DAFNNB010000375          204 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.denovo.6200, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000375 DAFNNB010000000
VERSION     DAFNNB010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..204
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.denovo.6200"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..>204
                     /locus_tag="L3M60_003655"
     rRNA            <1..>204
                     /locus_tag="L3M60_003655"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           46 a           44 c           61 g           53 t
ORIGIN      
        1 attggtgaca ggtggtgcat ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc
       61 ccgcaacgag cgcaaccctt gtctttagtt gccatcatta agttgggcac tctagagaga
      121 ctgccaggga taacctggag gaaggtgggg atgacgtcaa atcatcatgc cccttatgct
      181 tagggctaca cacgtgctac aatg
//
LOCUS       DAFNNB010000376        20147 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0062, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000376 DAFNNB010000000
VERSION     DAFNNB010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0062"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..396)
                     /locus_tag="L3M60_003656"
     CDS             complement(<1..396)
                     /locus_tag="L3M60_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HBN6228241.1"
                     /translation="MNKLSDEIINSLSTTIREKVEKISNNNLNIEEIRLRSQKPLILN
                     ANSKDYFYNQKTMALDLNQQNSYVVTREDVEQTFQIICKYSIHSFMDDIKKGFITLRG
                     GHRVGLVGKVIVEDGQVKNIKHISSLNIRV"
     gene            825..1139
                     /locus_tag="L3M60_003657"
     CDS             825..1139
                     /locus_tag="L3M60_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBN6228242.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            1155..1541
                     /locus_tag="L3M60_003658"
     CDS             1155..1541
                     /locus_tag="L3M60_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HBN6228243.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            1570..2955
                     /locus_tag="L3M60_003659"
     CDS             1570..2955
                     /locus_tag="L3M60_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBN6228244.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            2958..3110
                     /locus_tag="L3M60_003660"
     CDS             2958..3110
                     /locus_tag="L3M60_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN6228245.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            3133..4338
                     /locus_tag="L3M60_003661"
     CDS             3133..4338
                     /locus_tag="L3M60_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HBN6228246.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            4381..4602
                     /locus_tag="L3M60_003662"
     CDS             4381..4602
                     /locus_tag="L3M60_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228247.1"
                     /translation="MNFGQNLYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLI
                     IAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            4719..5216
                     /locus_tag="L3M60_003663"
     CDS             4719..5216
                     /locus_tag="L3M60_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBN6228248.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            5191..5697
                     /locus_tag="L3M60_003664"
     CDS             5191..5697
                     /locus_tag="L3M60_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN6228249.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            5681..8128
                     /locus_tag="L3M60_003665"
     CDS             5681..8128
                     /locus_tag="L3M60_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBN6228250.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            8131..10308
                     /locus_tag="L3M60_003666"
     CDS             8131..10308
                     /locus_tag="L3M60_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HBN6228251.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            10305..11306
                     /locus_tag="L3M60_003667"
     CDS             10305..11306
                     /locus_tag="L3M60_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HBN6228252.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLGNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            11303..12235
                     /locus_tag="L3M60_003668"
     CDS             11303..12235
                     /locus_tag="L3M60_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002989471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBN6228253.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            12480..12596
                     /locus_tag="L3M60_003669"
     CDS             12480..12596
                     /locus_tag="L3M60_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HBN6228254.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            12612..14531
                     /gene="tet(M)"
                     /locus_tag="L3M60_003670"
     CDS             12612..14531
                     /gene="tet(M)"
                     /locus_tag="L3M60_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HBN6228255.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            14650..14817
                     /locus_tag="L3M60_003671"
     CDS             14650..14817
                     /locus_tag="L3M60_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HBN6228256.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(14877..15230)
                     /locus_tag="L3M60_003672"
     CDS             complement(14877..15230)
                     /locus_tag="L3M60_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6228257.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            15735..16157
                     /locus_tag="L3M60_003673"
     CDS             15735..16157
                     /locus_tag="L3M60_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HBN6228258.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            16154..16384
                     /locus_tag="L3M60_003674"
     CDS             16154..16384
                     /locus_tag="L3M60_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBN6228259.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            16845..17048
                     /locus_tag="L3M60_003675"
     CDS             16845..17048
                     /locus_tag="L3M60_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HBN6228260.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            17130..18347
                     /locus_tag="L3M60_003676"
     CDS             17130..18347
                     /locus_tag="L3M60_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBN6228261.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            complement(18661..>20147)
                     /locus_tag="L3M60_003677"
     CDS             complement(18661..>20147)
                     /locus_tag="L3M60_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003428429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HBN6228262.1"
                     /translation="EEYYKSIVETIVDIGIAKEFIIAISTVIQKLVVDRLHVIGDIYD
                     RGPRPDIIVDKLIEHHCVDIQWGNHDILWMGAASGEKTCIANALRISARYANLDIVED
                     IYGINLLPLATFAIEMYKDDPCKEFIPKINDQSVTTTEKSLMAKMHKAISIIQFKLEG
                     EVIRRRPEFEMEHRLLLNMINYDEGTITLKGKTYKLKDTYLPTIDKKDPYKLTIEEKN
                     VIDKLVSSFRGSEKLQKHVSFLFSKGSIYLKANSNLLIHGCVPLNEDGSFMSMNIMGK
                     EYKGKALMDRMESLAREGFFFKDKAEEKLYGMDIMWYLWTGKCSSLFGKDDMTTFERY
                     FIAEKETHKENKNPYFKLRENEMACKKLFEEFDLELDESHIINGHVPVESKNGESPIK
                     ANGKILVIDGGFSRAYQKTTGIAGYTLIYNSRTLQLVSHEPFNSAEEAIANESDILST
                     TVVVEHKAKRKMVRDTDEGVKIQEEIEDLKLLLMAYKKGLIKEM"
BASE COUNT         6545 a         3605 c         3986 g         6011 t
ORIGIN      
        1 tacccttata tttaaagatg atatatgctt tatatttttg acttgtccat cttctactat
       61 tactttccca actaatccta ctctatgacc tcctctcaaa gttataaatc ctttttttat
      121 gtcatccata aatgaatgta ttgaatattt acatattatt tgaaaagttt gctctacatc
      181 ttctcttgtt actacatatg agttttgttg atttaaatcg agtgccatag ttttttgatt
      241 gtaaaaataa tctttagagt ttgcatttag tatcaatggt ttttgtgagc gtaacctaat
      301 ttcttctatg tttagattat tgtttgagat tttttctacc ttttctctta tagttgtaga
      361 aagagagttt attatttcat cagaaagttt attcatttct tgtcctcctc ctttgtttat
      421 ataattctat ttataaagga caaggaatat aaccaaaaca tctatatttt tttataatgt
      481 tttagattaa aaatagcata aaaatctagt tatccgcata aaaactggac ttatcacact
      541 ttatcaaggt caaaaccact caatttacta ctaatttact acttatgaat gagctttgat
      601 acgacgattt atccttgaaa agtgaagata taaagatact tccaataaaa tttgaatatt
      661 taataggtag acacttcaaa aaatgaggtg tctatttttt tacccgattt tgaaaggaag
      721 tgaacttatg aaaacaaaaa atcaagaatc aaaaggtcgt tccccactct ttaagaccat
      781 caaacattca ttcagccaat aaaaaagaaa ggataggtaa aaatatggaa cttaaatttg
      841 tgattcccaa catggaaaaa acattcggca atttagaatt tgctggcgag gataaagtcg
      901 ttcagcgaag aatcaacgga cggctaactg tcttatcaag aagctataat ctctattctg
      961 atgttcaaag agcagatgat attgtggtgg tgcttcctgc tgaagctggc gaaaaacatt
     1021 tcggctttga ggaacgtgtg aagttagtca atccacgtat taccgcagag ggctacaaaa
     1081 tcggcactcg tggttttaca aattaccttt tacatgctga cgacatgata aaagaataaa
     1141 gaaagagagg aaaaatgatg agattagcaa atggcattgt attagataaa gacacgactt
     1201 ttggagaatt gaaattctct gctctacgtc gtgaagtgag aatccaaaat gaagacgggt
     1261 cggtttcaga tgaaatcaag gaacgtacct atgacttaaa atccaaagga caaggacgca
     1321 tgattcaagt aagtattcct gccagcgtgc ctttgaaaga gtttgattat aacgcacggg
     1381 tggaacttat caatcccatt gcggacaccg ttgctactgc cacctatcaa ggagcagatg
     1441 ttgactggta tatcaaggca gacgatattg tgctgacaaa ggattctagt tcattcaaag
     1501 ctcaaccaca agcaaagaaa gaaccgacac aagacaaata gtcgctaggt agaaaggaga
     1561 ctttttcgca tgaaacagcg tggtaaaagg attcgcccat ctggtaaaga tttagtcttt
     1621 cattttacga tagcgtcact cctgcctgtt ttcctgctgg ttgtcggact gtttcatgtg
     1681 aagacaatcc agcagatcaa ctggcaggat tttaacctat cacaagcaga taagattgac
     1741 attccctatt taattatcag tttcagtgtc gcaattctta tctgcttgct ggtagcgttt
     1801 gtattcaaac gggttcgcta tgatacggtt aaacaacttt accaccgtca aaaactggca
     1861 aagatgatac ttgaaaacaa gtggtatgaa tctgaacagg tcaaaacaga gggtttcttt
     1921 aaagatagtg ctggtcgtac aaaggaaaag ataacctact tccctaaaat gtattatcga
     1981 cttaaaaatg gcttgataca gatacgggtg gaaatcacgc tgggaaaata tcaagaccaa
     2041 ctcttacact tggaaaagaa attagagagt ggcttgtact gtgagctgac ggataaagag
     2101 ttaaaggatt cctatgtgga atatactttg ctctatgaca ccatagccag tcgtatttct
     2161 attgatgaag tagaagctaa agatggtaaa cttcgcttaa tgaaaaacgt atggtgggaa
     2221 tatgataagc tccctcatat gttgattgct ggtggtacag gtggcggtaa aacttacttt
     2281 atactgacac tgattgaagc cttgcttcat acagattcaa aactgtatat tcttgacccg
     2341 aaaaatgctg accttgcgga cttaggttct gtgatggcaa atgtctacta tagaaaagaa
     2401 gacttgcttt cttgcattga aacattctat gaagaaatga tgaaacgtag tgaggaaatg
     2461 aagcagatga agaactataa gactggcaaa aattatgctt acttaggtct cccggcacac
     2521 ttcttaatct ttgatgaata cgtcgctttc atggaaatgc tgggaacaaa agaaaacacc
     2581 gcagttatga ataagctgaa acagattgtc atgttaggtc gtcaagctgg cttctttcta
     2641 atactggctt gtcaacgtcc agacgcaaaa tatttaggcg acggaatccg tgatcagttt
     2701 aatttcagag tggctttagg tcgtatgtct gaaatgggct atggcatgat gtttggcagt
     2761 gacgtacaaa aggatttctt cttaaagcga atcaaaggtc gtggctatgt tgatgtagga
     2821 acaagtgtca tatcagagtt ttatactccc cttgtaccaa aaggatatga tttcttggag
     2881 gaaattaaaa agttatccaa cagcagacag tccacgcagg cgacgtgcga agcggaagtc
     2941 gcaggtgtgg actgatcttg ctggctggtg tggcaatagc cacgccagca cttaaccccc
     3001 cgtatctaac aggggggtac aaatcgacag gaaacagtca aaaaaacatt agaaaatcct
     3061 ttggttacaa gggatttaca aaatttcagc gtatgtcaaa tgggctttaa aagttgacat
     3121 acgccttttt gattggaggg atttttactg aatgaacaaa cttggttaca gcatttaaaa
     3181 gaaaaacgct tggcttatgg actatctcaa aaccgtttag ctgttgcgac tggtattaca
     3241 aggcagtatc taagcgatat tgaaacagga aaagtcaagc catcagagga tttacagcag
     3301 tccctttggg aagctctgga acgcttcaat cccgacgctc cccttgaaat gctgtttgat
     3361 tatgtaagga ttcgctttcc gacaacagac gtacagcagg tggtcgaaaa catcttacaa
     3421 ctgaaactgt cctattttct tcatgaggac tatggtttct attcttattc agagcattat
     3481 gctttaggcg acatattcgt cctttgctcc catgaactgg acaaaggagt tctggtggaa
     3541 ttgaaaggtc gtgggtgcag acaatttgaa agctatcttc tggcacaaca aagaagctgg
     3601 tatgagttct ttatggacgt tttggtggct ggcggtgtga tgaaacgcct tgaccttgcc
     3661 attaacgata agacagggat tttgaatatc cctgtactca ctgaaaagtg ccaacaggaa
     3721 gaatgtatct ccgtcttccg cagttttaaa agctatcgca gtggcgaact ggtacgcaaa
     3781 gaggaaaagg aatgtatggg aaacaccctc tatatcggtt cattacaaag tgaagtttat
     3841 ttctgtatct atgaaaagga ctacgagcag tacaagaaaa atgatattcc cattgaagac
     3901 gcagaagtaa aaaaccgttt tgagattcga ttgaaaaatg agcgtgccta ttatgcagtc
     3961 cgtgatttac tcgtctatga caatccagag cataccgcct ttaaaattat caatcggtat
     4021 atccgttttg tagataaaga cgattccaaa cctcgttctg attggaaact gaatgaagaa
     4081 tgggcttggt ttattgggaa caatcgtgaa cgattaaaac taaccacaaa accagagcct
     4141 tactccttcc aaaggacgct gaactggcta tctcatcaag ttgccccgac cttaaaggtt
     4201 gcgattaaac ttgatgaaat caaccagacg caggttgtaa aagacattct cgaccatgcg
     4261 aaactgacag accgacacaa gcagattttg aagcaacagt cagtaaaaga acaggacgtg
     4321 ataacaacaa aaaaataact caaatacaaa ttcattgaat atagagagga gaacattttt
     4381 atgaattttg gacaaaacct ttataactgg tttctatcaa acgctcaatc actggtgctt
     4441 ttagcaatcg ttgtgattgg cttgtatctt ggcttcaagc gtgagtttag caaactgatt
     4501 ggctttttaa ttattgcgat tattgcggtt ggcttagtct tcaacgctgc tggagtaaaa
     4561 gacattttac tagagctatt caatcgcatt attggtgctt aaataaaacc gttcttttgt
     4621 ggaatataag tggttttctt atgttccgca aaggaatggt acaccaaacg aagtgcggta
     4681 gggatttttg aatctctaca aagaaaggac gtgaatatat ggacgatatg caagtctata
     4741 ttgcgaattt aggcaaatac aatgagggcg aattggtcgg tgcgtggttt acctttccca
     4801 ttgactttga ggaagtcaaa gagaaaatcg gcttgaatga tgaatatgag gaatacgcca
     4861 ttcatgacta cgagttaccc tttacggttg acgaatacac ttccattggc gaactcaatc
     4921 gactatggga aatggtatcg gaattacccg aagaattaca atcggagcta tctgctctgc
     4981 tcactcattt ttcaagcatt gaagaactaa gcgaacatca agaggatatt atcattcatt
     5041 ccgattgtga tgatatgtat gacgtggcac gctactacat tgaagaaacg ggtgctttag
     5101 gcgaagtacc agctagtctt caaaactata ttgattatca agcctatggt cgggatttag
     5161 acctttcagg aacgtttatc tcaaccaatc atgggatttt tgaaatcgtc tattaaatct
     5221 gtcggtacat tactactggc agattttcta ttttacgggg tggctcaatc agctacccct
     5281 attttttatg aaaggattga ttacatgaag aaaatacgaa gctataccag tatctggtct
     5341 gtggaaaagg tactgtattc tatcaatgat tttagacttc cgtttcccat aacctttacg
     5401 caaatgacat ggtttgtcgt gtcactcttt gcagtgatga tacttggcaa cttgccccct
     5461 ctttccatga tagagggagc atttctcaaa tactttggga ttcctgtggc tttcacatgg
     5521 tttatgtcta caaaaacttt tgatggtaaa aagccttatg gatttttgaa gtctgtcatt
     5581 gcttatgcac tgcgaccaaa gctgacctat gcaggaaaaa aagtaacgct tggcagaaac
     5641 cagccacaag aagccattac agcagttagg agtgaatttt atggcatatc caattaaata
     5701 cattgaaaac aatctcgtct ggaataaaga cggggaatgt tatgcttact atgagcttgt
     5761 tccttacaat tactcatttc taagtccaga acagaaaata caagtgcatg attctttcag
     5821 acagcttatc gcacaaaatc gtgatggcaa aattcatgct ttacaaatca gtacagaatc
     5881 cagcatacgt tctgcacaag agcgttccaa aaatgaagtc actggcaagc tcaaagcggt
     5941 tgcctatgac aaaatcgacc aacagacaga cgctttaata tccatgattg gcgaaaatca
     6001 agtgaactac cgtttcttta tcggctttaa gttgcttctc aacgatcagg agttttctat
     6061 gaaaagtctt accgttgaag caaaaaatgc tttgtctgat tttgtctatg atgtgaacca
     6121 taagctgatg ggcgattttg ttagtatgag taatgatgaa atcctgcgtt ttcagaagat
     6181 ggaaaagctc ttagaaaata aaatctctcg tcgtttcaaa atccgcaggt tagataagga
     6241 cgacttcggc tatctgattg aacaccttta cggacagaca ggcactgcct atgaagagta
     6301 tgagtaccat ctatcaaaga aaaagctgga taatgaaacg ctgattaaat actatgactt
     6361 gattaagcct actcgctgtt tggtggaaga aaaacagcga tatttgaaaa tccagcagga
     6421 agatgaaacc gtctatgtag cttactttac cattaacagc attgtcggag aactggactt
     6481 cccgtcctct gaaatcttct actaccagca acagcaattt acattcccga ttgatacgtc
     6541 aatgaatgtg gaaattgtag cgaatcgtaa agccctatct actgtccgca ataaaaagaa
     6601 agaactgaaa gacttggata accacgcttg gcaaagtgat aatgaaacca gctccaatgt
     6661 ggcggaagct ctggaaagtg tgaatgagct ggaaaccaat ttagaccaaa gcaaggaatc
     6721 tatgtacaag ctgtcttatg tggtaagggt atcagcaaat gatcttgacg aactcaaacg
     6781 tcgttgtaat gaagtgaaag atttttatga cgatttaagc gtaaaactgg tacgaccatt
     6841 tggggatatg ctcggcttac atgaagaatt tttacctgcc agcaagcgtt atatgaatga
     6901 ttatattcaa tacgtgacct ctgatttcct cgctggttta ggttttggtg ctactcaaat
     6961 gctgggggaa aatgagggga tttatgttgg ctacagctta gatactggac gcaatgtcta
     7021 tctgaaacct gctcttgcca gtcaaggggt taagggttca gtaaccaatg cgttagcgtc
     7081 ggcttttgtt ggttcgctgg gtggtggtaa atcctttgcg aataacctta tcgtctatta
     7141 tgcggtgctt tatggggcac aagcagtgat tgtagaccca aaagcagaac gtggcagatg
     7201 gaaagaaacc ttgccagaga tttcccatga aatcaatatc gtcactctga cttctgatga
     7261 gaaaaacaaa ggcttacttg acccttatgt gattatgaaa aatcccaaag attctgaatc
     7321 actggctatt gatattctga cattccttac ggggatttcc tctcgtgatg gggaacgctt
     7381 cccaatcctt agaaaagcca ttcgtgcagt aaccaatagt gaagtacgag ggttgatgaa
     7441 agtgattgag gaattacggg ttgagaatac gccactaagt accagtatag ccgaccatat
     7501 cgaaagtttt acagactatg actttgcaca tttattattc agtaatggtt atgtggagca
     7561 gtctatcagc ttagaaaaac aactgaacat tatacaggtt gcggacttgg tacttcccga
     7621 caaggaaact tcctttgagg aatataccac tatggagctt ttatccgttg ctatgctgat
     7681 tgtcattagt acctttgctt tagactttat ccatacagac cgaagcattt tcaagattgt
     7741 agatttagac gaagcatgga gctttttaca ggtagcacaa ggaaaaacac tatctatgaa
     7801 gctggttcgg gctggtcgtg ctatgaacgc tggggtatat ttcgtgaccc aaaatacaga
     7861 cgacctctta gatgaaaaac tgaaaaataa cctcggctta aaatttgcat ttcgttccac
     7921 tgaccttaac gagattaaaa agaccttagc cttttttggt gtagacccag aggacgaaaa
     7981 caatcagaag cgattgcgtg atttggaaaa cgggcaatgc cttatcagtg atttatatgg
     8041 tcgtgtcggt gtgatacagt tccaccctgt atttgaagaa ctgctccatg cctttgatac
     8101 cagaccacct gtgcgaaaag aggtgtaaat gtgaaaccat caatagtaaa cagaataaaa
     8161 tcaaactgga cgctgaaacg tctaggtaaa gtggcaatga cagtggcttt cacacttgtg
     8221 attgccattt ttcttttagc catgctggga acggtggttc aagctgcggg cttggtagat
     8281 gatacggtca atgtggcaaa tgaatacagc cgatacccac ttgaaaacta tcaactggat
     8341 ttttatgtgg ataatagctg gggctggctt ccgtggaact ggtcggacgg gattggaaaa
     8401 caggtcatgt atggactata tgccattacc aattttattt ggacaatcag tttgtatgtt
     8461 tccaatgcga caggttactt agtacaggaa gcctattcct tagacttcat ttccgctaca
     8521 gcagattcca ttggtaagaa tatgcagacc ttagctggtg tgagtgcaaa cggattttca
     8581 acagagggtt tctatgttgg attcctctta ctcttgattt tggttcttgg ggtttatgtt
     8641 gcctatacgg gactgataaa gagagaaacc acaaaggcaa ttcatgccat tatgaatttt
     8701 gtgctggtgt ttatcctatc ggcttccttt attgcctacg ctcccgacta cattaaaaaa
     8761 atcaatgact tttcatcaga catcagtaat gccagtttat cacttggcac gaagattgtc
     8821 atgccccatt ccgatagtca aggcaaggac agcgtggact taatcagaga tagcctgttt
     8881 tccatacagg ttcagcaacc gtggctactg cttcaataca acagttcaga cattgaaagt
     8941 atcggtattg accgtgtgga aagcctgctc tccaccagcc cagattccaa caatggcgaa
     9001 gacagagaaa aaattgttgc ggaagaaatt gaagacagaa gcaataccaa tctaaccatt
     9061 acaaagacca ttaaccgttt aggtacagtc ttcttcctat ttgtcttcaa tattgggatt
     9121 tccatatttg tattcctatt aacaggaatc atgattttct cgcaggtact ttttatcatc
     9181 tatgctatgt ttctgcctgt gagctttatt ttaagcatga ttccatcatt tgatggtatg
     9241 tcaaaacgag ccataacaaa gctctttaat accattttga cacgagctgg aatcacattg
     9301 attattacga cagcatttag tatttcaacc atgctctata ccttatcggc tggttatccg
     9361 ttctttttga ttgcttttct acagattgtg acctttgcag gaatctactt caagctgggc
     9421 gatttaatga gtatgttttc tctacagagt aacgattctc aaagtgtggg aagtcgtgtg
     9481 atgagaaaac ctcgtatgct tatgcacgct cacatgcacc gtctacagcg gaaacttgga
     9541 cgttccatga ctactctagg ggctgggtct gccattgtta caggtaaaaa aggacagtcg
     9601 ggttcgggga gttctgcaag gacacaagca gatcactccc gaccagacgg aaaggaaaaa
     9661 tcaacacttg gaaaacgtat cggtcaaacc atcggtacag tagctgatac caaagacaga
     9721 atggtagaca ctgctagtgg tttgaaagaa caggttaaag atttgccgac caatgcaaga
     9781 tatgcagtat atcaaggaaa atccaaagta aaagagaatg tccgtgattt aaccagtagt
     9841 atttctcaaa ccaaagcgga cagagccagt ggacgcaagg aacagcagga acaaaggcga
     9901 aaaaccattg cgaagcgtcg ctctgaaatg gaacaggtca aacagaaaaa acagcctgct
     9961 tcttctgttc atgaaagacc gactacaaga caagaacaat atcatgatga acagacctca
    10021 aaacagtcta atattcagac ttcatataag gaatctcaac aagccaaaca agagcgtcca
    10081 gcagttaagt ccgatttttc aagtccaaaa gtggaacgcc aaggcaatac cgttcaagaa
    10141 aaaaccgttc aaaagccagc aacttcaacc actacagcag atagaacttc acaacgtcca
    10201 atcacaaaag aacgtccgtc tactgttcaa agagtaccac tacaaaatac aagaagtaga
    10261 ccaccaatca aaaccgccac cattaagaaa gtcggtaaga aaccatgaag ttgaaaactt
    10321 tagtgattgg tggttctgga ttattcttga tggtcttctc actgcttctg tttgttgcca
    10381 ttttattttc agatgaacag gacagcggaa tttccaatat tcattatgga ggtgtgaatg
    10441 tttccgcaga agtgctggct cataagccta tggtagaaaa atatgccaaa gaatatggcg
    10501 ttgaagaata tgtcaacata cttcttgcga ttatacaggt ggaatcgggc ggtactgcgg
    10561 aagatgttat gcagtcctcg gaatccctcg gtcttccacc taattcattg agtacagaag
    10621 aatccattaa gcaaggtgtg aagtatttca gtgaattatt agccagtagc gaaaggctca
    10681 gtgtagattt agaatcggtt atccagtcct acaattatgg tggtggtttc ttagggtatg
    10741 tggctaatcg tggaaataaa tatacctttg aactggctca aagtttctca aaagagtatt
    10801 caggtggcga aaaagtgtct taccccaatc ccatagccat acctatcaat gggggctggc
    10861 gatacaacta tggcaatatg ttttatgtgc aactggtaac gcagtatctt gtcacaacag
    10921 agtttgatga tgatacggta caagccatca tggacgaagc actgaaatat gagggctggc
    10981 gatacgttta cggtggagct tccccgacta cttcttttga ttgtagcgga ctgacacaat
    11041 ggacgtatgg aaaagctgga attaacttac cacgaaccgc acaacagcaa tatgatgtga
    11101 cccagcatat cccactatcg gaagcacaag ctggcgattt ggttttcttt cattctacct
    11161 ataacgctgg ctcttatatt actcatgttg ggatatacct tggcaataac cgtatgtttc
    11221 atgcaggcga cccaatcggt tatgccgact taacaagccc ctactggcaa cagcatttag
    11281 tgggagcagg acgaatcaaa caatgagaaa ggaagattta atgatgaaat ttagaaaaaa
    11341 tcagaataaa gaaaaacaga taccaaagga aaagaaacct cgtgtctata aggtcaatcc
    11401 tcataaaaag gttgtgattg ccttgtgggt acttttaggg cttagtttca gctttgcgat
    11461 attcaagcac tttacagcta tagatactca tactattcac gaaacaacta tcatagaaaa
    11521 ggaatacgtt gatactcatc atgtagaaaa ttttgtagag aactttgcga aagtctacta
    11581 ttcatgggag caatccgata agtccattga taatcgaatg gaaagtctaa aaggctatct
    11641 gacagatgaa cttcaagctc tcaatgttga tacagtacgc aaagatattc ctgtatcgtc
    11701 ttctgtaaga ggatttcaga tatggacggt agagccaact ggcgacaatg agtttaatgt
    11761 aacctacagt gtagaccagc tcattacaga gggagaaaat acaaagaccg tccactctgc
    11821 ttatatagtg agtgtctatg tagatggttc tggaaatatg gtactggtta agaatccgac
    11881 cattaccaac atacctaaga aatcaagtta taaaccaaaa gccattgaaa gtgaggggac
    11941 ggttgattcc attacaacca atgaaatcaa tgagttttta acgacgttct tcaagctcta
    12001 tcctacagcg acagccagtg aactttccta ctatgtgaat gacgggatat taaaaccaat
    12061 cggaaaagag tacatctttc aagaactggt aaatcctatt cacaatcgta aggataatca
    12121 agtcacggta tcgctgacag tggagtatat cgaccagcag accaaagcaa cgcaggtatc
    12181 tcaatttgat ttggtacttg aaaagaacgg gagtaattgg aagattatag aataacaaat
    12241 attggtacat tattacagct attttgtaat cacgtactct ctttgataaa aaattggaga
    12301 ttcctttaca aatatgctct tacgtgctat tatttaagta tctatttaaa aggagttaat
    12361 aaatatgcgg caaggtatta ttaaataaac tgtcaatttg atagcgggaa caaataattg
    12421 gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac ctttatcatg
    12481 tgattctaaa gtatccggag aatatctgta tgctttgtat gcctatggtt atgcataaaa
    12541 atcccagtga taagagtatt tatcactggg atttttatgc ccttttgggc ttttgaatgg
    12601 aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat gcaggaaaaa
    12661 ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta ggaagcgtgg
    12721 acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga attacaattc
    12781 agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata gacacgccag
    12841 gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat ggggcaattc
    12901 tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt catgcactta
    12961 ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat ggaattgatt
    13021 tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta atcaaacaga
    13081 aggtagaact gtatcctaat gtgtgtgtga cgaactttac cgaatctgaa caatgggata
    13141 cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa tcattagaag
    13201 cattggaact cgaacaagag gaaagcataa gatttcagaa ttgttctctg ttccctcttt
    13261 atcatggaag tgcaaaaagt aatataggga ttgataacct tatagaagtt attactaata
    13321 aattttattc atcaacacat cgaggtccgt ctgaactttg cggaaatgtt ttcaaaattg
    13381 aatatacaaa aaaaagacaa cgtcttgcat atatacgcct ttatagtgga gtactacatt
    13441 tacgagattc ggttagagta tcagaaaaag aaaaaataaa agttacagaa atgtatactt
    13501 caataaatgg tgaattatgt aagattgata gagcttattc tggagaaatt gttattttgc
    13561 aaaatgagtt tttgaagtta aatagtgttc ttggagatac aaaactattg ccacagagaa
    13621 aaaagattga aaatccgcac cctctactac aaacaactgt tgaaccgagt aaacctgaac
    13681 agagagaaat gttgcttgat gcccttttgg aaatctcaga tagtgatccg cttctacgat
    13741 attacgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa gtacaaatgg
    13801 aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta aaagagccta
    13861 cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac atcgaagtgc
    13921 cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg ttgggaagtg
    13981 gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt caaaatgcag
    14041 ttatggaagg gatacgctat ggttgtgaac aaggattgta tggttggaat gtgacggact
    14101 gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca gcagattttc
    14161 ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca gaattgttag
    14221 agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga gcatacaacg
    14281 atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat gaggtcattc
    14341 ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta actttcttta
    14401 caaatggacg tagtgtttgt ttaacagagt taaaagggta ccatgttact accggtgaac
    14461 ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa agtacgatat atgttcaata
    14521 aaataactta gtgtatttta tgttgttata taaatatggt ttcttgttaa ataagatgaa
    14581 atatttttta ataaagattt gaattaaagt gtaaaggagg agatagttat tataaactac
    14641 aagtggatat tgtgtcctgt atgtggaaat aaaacacgat taaagataag ggaagatact
    14701 gaattaaaaa aattccccct ctattgtccg aaatgcagac aagaaaattt aattgaaata
    14761 aagcagttca aagtaactgt gattacagag ccagacgcaa agacgcagag ccgataaaat
    14821 gagattaata caatctcatt ttatcggctc tttccgttat gtatggattc ttttaattag
    14881 tcttcgatgt ttcttgcttc gttgataccg ctggctaaag attccattaa ggatagttct
    14941 ttgtctgtaa agctatccat gtatttctct atctgtaatc gtcgggtgct ttttaccaag
    15001 ttattagcag gtaagaaaaa ttcatcaacg gaaacatgaa gtaacgatac aaggtcataa
    15061 agaacttgta tgctggggtg ttgcccttta ttttcaatat tagttaagta ccgtgggtca
    15121 atttcaatca atgctcccac ttgttcacga gttaaacctc gtttcaatcg agcttcttta
    15181 atggctaaac caaaggctct aaaatcatat ttatcttctt ttttacgcat agtagaccac
    15241 ctctatacat tttattgttc ctactgaatt aaaaacaggt atagaaaaac gtgttatatg
    15301 gtttataggt ttatatttaa taaaaagcac tactaaacgc caataaaaaa aaccgttata
    15361 tggtagtgct atttacgctg ttaaaatatt gtatattact tccaaatggc ggtttgttgg
    15421 aggtcaacgt cgccatgaag tacatcatat acaataaatt tccttacatt gggttcttgt
    15481 caaaaaaagt cgtctatctg caatagataa gtacgtccac caatgtggtt ttataaatca
    15541 tatagataga ataacagaag catgtaaaca gagaaataaa tctgtttata tgcttttttg
    15601 gctattcaga acttttttac aaagtttatt tatcagtaat gcaacaaatc cccctttcac
    15661 attgggacta agagtgaaag gagataaacg agcaaggctc acttcctttc ctagacagaa
    15721 agggggtgag aaacatgaaa ccatcttctt ttcagaccac aatagaaaat cagtttgact
    15781 atatctgtaa acgtgctatg gaagacgagc gaaagaatta tatgctttat ctttcaagga
    15841 ttgcaaagcg tgaggtgtcc ttttcggatg ttggcgatta tcttgttagc cagtttgcga
    15901 caacagataa ctattcaact gactttcaga tttttacact caatgggtta tcagtaggcg
    15961 ttgaaaatga tttgttgagt gaagcattac gtgagttgcc agacaagaaa cgtgaaattc
    16021 tactgctgtt ttactttatg gacatgagcg attcagaaat tgcagacctg ttgaaattga
    16081 accgttctac tgtctatcgg catagaacca gtggactagc cttaattaaa aagtttatgg
    16141 aggaatttga agaatgaaaa cacaatatcc tatgattccc tttcctctca ttgtaaaggc
    16201 aacagatggc gataccgaag cgattaacca gattctacat cattacagag ggtacataac
    16261 gaagcgttcc ctacgactta tgaaagatga atatggcaat caaagtatgg tcgttgatga
    16321 agtcttacgt ggaagaatgg aaaccagact gattacaaag attttgtcat ttgaaattaa
    16381 gtaatatcct ctctcctttc gtggaagcgt gctaaaccat tccacgcttc ccgaacaggg
    16441 aggtttgtta ttccaccaaa gcatattgag ctttcaatgt gttttgatag gctaacgagc
    16501 cattgttctt tgaaaactga ataaaagtaa tcgaatacgt ttcgataaga aaagagccaa
    16561 cggaactaac cgccatgacc tatcttataa agatagcgag cgattcatgt tagtgatccg
    16621 agaagcaatc tttagcagga ttgcctgcaa cgacattctt atcgtgataa tgatactccc
    16681 atacagtcaa tagtccgagc gtgataaaac cgtcgcaggc aatgagtatg gctacatgag
    16741 aaccatgcag gggtggaact cccgtgagct ttgctaaagc tgttcgattg ctggtaaaac
    16801 aacttttatg aaatccaaat aagtgatttg gaaaggagga ttttatgaag cagactgaca
    16861 ttcctatttg ggaacgttat accctaacca ttgaagaagc gtcaaaatat tttcgtattg
    16921 gcgaaaacaa gctacgacgc ttggcagagg aaaataaaaa tgcaaattgg ctgattatga
    16981 atggcaatcg tattcagatt aaacgaaaac aatttgaaaa aattatagat acattggacg
    17041 caatctagcg tcgccaaagg gtcttgtata tgataaaata gtattaagtc gtatcaaggc
    17101 tctttccata aaggaaagga gcaaatgcca tgtcagaaaa aagacgtgac aataaaggtc
    17161 gaatcttaaa gactggagag agccaacgaa aagacggaag atacttatac aaatatatag
    17221 attcatttgg agaaccgcaa tttgtttact cgtggaaact tgtggctaca gaccgagtac
    17281 cagcaggaaa gcgtgattgt atctcactta gagagaaaat cgcagagtta cagaaagaca
    17341 ttcatgatgg tattgatgtt gtaggaaaga aaatgacact ctgccagctt tacgcaaaac
    17401 agaacgctca aagaccaaag gttagaaaaa acactgaaac tggacgcaaa tatcttatgg
    17461 atattttgaa gaaagacaag ttaggtgtaa gaagtattga cagtattaag ccatcagacg
    17521 ctaaagaatg ggctattaga atgagtgaaa atggttatgc ttatcaaacc atcaataact
    17581 acaaacgttc tttaaaggct tcattctata ttgctataca agatgattgt gttcggaaga
    17641 atccatttga ctttcaactg aaagcagttc ttgatgatga tactgtccct aagaccgtac
    17701 taacagaaga acaggaagaa aaactgttag cctttgcaaa agctgataaa acctacagca
    17761 aaaattatga tgaaattctg atactcttaa aaacaggtct tcgtatttca gagtttggtg
    17821 gtttgacact tccagattta gattttgaga atcgtcttgt caatatagac catcagctat
    17881 tgagagatac tgaaattggg tactacattg aaacaccaaa gaccaaaagt ggcgaacgtc
    17941 aagttcctat ggttgaagaa gcctatcaag catttaagcg agtgttagcg aatcgaaaga
    18001 atgataagcg tgttgagatt gatggatata gtgatttcct ctttcttaat agaaagaact
    18061 atccaaaagt ggcaagtgat tacaacggca tgatgaaagg tcttgttaag aaatacaata
    18121 agtataacga ggataaattg ccacacatca ctccacatag tttgcgacat acattctgta
    18181 ccaactatgc aaatgcagga atgaatccaa aggcattaca gtacattatg ggacatgcta
    18241 atatagccat gacgctgaac tattacgcac atgcaacatt cgattctgca atggcagaaa
    18301 tgaaacgctt gaataaagag aagcaacagg agcgtcttgt tgcttagtag tacaaatgaa
    18361 tttactactt atttaccact tctgacagct aagacatgag gaaatatgca aagaaacgtg
    18421 aagtatcttc ctacagtaaa aatactcgaa agcacataga ataaggcttt acgagcattt
    18481 aagaaaatat aaaaagataa ttagaaattt atactttgtt ttagattaaa aaaagtgaac
    18541 ccatttatct tggattcact tagtaagtat aaggttataa attactatta ttaatctata
    18601 taatatattt aataagtatt aacaaaaaga cacttcaatt atgaagtgcc tctatagtat
    18661 ctacatttct tttattagcc ctttcttata agccattaaa agtagtttta aatcttctat
    18721 ttcctcttgt atttttactc cttcatcagt atctctcacc atttttctct ttgccttatg
    18781 ttccactaca acagttgttg ataagatatc actttcattt gcaatggctt cttcagcaga
    18841 attaaatggt tcatgcgata ctaattgcaa agttctagaa ttgtatatca aagtgtatcc
    18901 agctatacca gtagtttttt ggtaagctct agaaaaacca ccatctataa cgagaatttt
    18961 accattagct tttattgggc tctctccatt tttactctct acaggaacat gaccatttat
    19021 tatatgagat tcatcaagtt ctaaatcaaa ttcttcaaat aattttttac aagccatctc
    19081 attttctcta agtttaaaat atggattctt attttcttta tgagtctctt tttccgctat
    19141 aaagtatctt tcaaatgtag tcatatcatc tttaccaaat aatgatgaac atttcccagt
    19201 ccataaatac cacataatat ccatgccata cagtttttct tctgccttat ctttaaagaa
    19261 aaatccttct cttgctagag actccattct atccataagg gctttacctt tatactcttt
    19321 gcccattata ttcatagaca taaagcttcc atcttcattt aatggtacgc atccatgaat
    19381 cagtaaattt gagttagctt ttaaatatat acttccttta gagaacagaa atgaaacatg
    19441 tttttgtaat ttttcacttc ctctaaatga agatactaat ttatcaataa catttttttc
    19501 ttctatagtt aatttatatg ggtctttttt gtctattgta ggcaaataag tatcttttaa
    19561 tttatatgtt tttcctttta aagtaatcgt accttcatca tagtttatca tattaagtaa
    19621 aagtctatgt tccatctcaa actcaggtct tcgtcttata acttcacctt caagcttaaa
    19681 ttgaattata cttattgctt tgtgcatttt tgccattaaa gacttttctg ttgttgttac
    19741 actttggtca ttaatctttg gaataaattc tttacaaggg tcatctttat acatctctat
    19801 cgcaaaagtg gctaaaggca atagatttat tccatatatg tcttctacaa tgtctaaatt
    19861 tgcatatcta gctgatatcc taagtgcatt tgctatacaa gttttttcac ctgatgctgc
    19921 acccatccat aagatatcat gatttcccca ttgaatgtcc acacaatgat gttcaataag
    19981 cttatcgaca attatatctg gtcttggacc tctatcataa atatctccta ttacatgaag
    20041 tctatctaca actaattttt gtataactgt agaaatagca attataaact ctttagctat
    20101 tcctatatca actattgttt caactatact tttatagtac tcttctt
//
LOCUS       DAFNNB010000377        10975 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0129, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000377 DAFNNB010000000
VERSION     DAFNNB010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10975
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0129"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            13..1419
                     /locus_tag="L3M60_003678"
     CDS             13..1419
                     /locus_tag="L3M60_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011860965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HBN6228263.1"
                     /translation="MNTGFPKDFLWGASSSAFQVEGAWDKDNKGKTVADYNSFKKSHL
                     QADTKVASDFYNNYEEDIELMKELGMKTYRFSISWARIIPDGEGEINQKGIDFYNKII
                     DKLIECDIEPFVTLYHFDLPFTLVEKYNGWESRETVYAFERFAKICFKHFGDRVKYWQ
                     PHNEQNLIVRVEERINIYDETDSWKIDKMRAQMDYNLCLAHALAVNACHEMIKESKIG
                     AAVSSSVTYPLTSKPEDVYAARMNDNFKVYYMLDMHHYGEYPGYYMRYLEKRNIVPHM
                     EDGDKEVLKKAKMDFIAVNYYRTNCAGAFPEDSQHPFGLREGTVDFSMYGLFKMSMNP
                     NLEASEYGAAIDPSGLRVALNEYWQRYHLPVIITENGLGAKDILEDGKIHDDYRIDYL
                     RSHINACRLAIEDGVEMIGYCPWSFTDLLSSSQGFNKRYGLVYINRTDHEVLDLKRIK
                     KDSFYWYKEVIENNGIVK"
     gene            1447..2295
                     /locus_tag="L3M60_003679"
     CDS             1447..2295
                     /locus_tag="L3M60_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004454252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBN6228264.1"
                     /translation="MYITKVLNNSLLLARDDNDEEIILMGKGIGHNYKAGSELNKKDI
                     EKIFVLHDENIKKSIIQLATEIDAEYFGIAQMIIAYGIEKYNLKLMNHIYLALTDHIA
                     FAVRRFKEGIMIENHYLFEIKEFNPKEYDIGKYAITVFKEVLGLELPEEEIGNIAGHF
                     INAQQDNPYSDRNKRSAKIVNAILQIVHYHFSIVYNKESFYYRRFVMHLKAFSQRFLA
                     NEPSKEKIDFIYEQVQKNCKEEYECVKKVGAYMYKEYSRELPRQEELYLMIHIHKILG
                     ELCENS"
     gene            2955..3647
                     /locus_tag="L3M60_003680"
     CDS             2955..3647
                     /locus_tag="L3M60_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003020373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6228265.1"
                     /translation="MNKVLIIDDDKDLCILLKKSISIEHIEANYCHSGRDGLVELSKQ
                     DYQLVVLDVMMPGMDGFETMEQIRKYSSIPILMLTSKNDNYSKVHGLRAGADDYLTKP
                     FEIAEFTARVLSLIRRYTRFNPKNEESQQLSFKGLLIDLDQHTVTTNGETMLLPPKEF
                     GVLLYCARHQGKILTKQQIYEAVWGEPYVYDDSNIMAIISRLRKKIELDPSSPIYLQT
                     IKGIGYQFNTEV"
     gene            3644..4579
                     /locus_tag="L3M60_003681"
     CDS             3644..4579
                     /locus_tag="L3M60_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6228266.1"
                     /translation="MKMDEQTIILFLFLLSIGLLFLSIFAFSKLGHIHKRLKDIEEIL
                     ADVQIGNENRKILIKPCDVMAPLVYQLNEIVYDYENKLLSLKKSDKASKQLMTSLSHD
                     VRTPLTTLIGYLDAVHSGIVIGQEREEYIEIARRRAYDLKDYIDVLFDWFRLNSDEFT
                     LSIESVEIAELSRNILKDWIPIFHEKKLDFEIDIPENRLMVNLDSDGYSRVVNNLVQN
                     VLAHSKARRIKITMSEDSKMVLLRVEDNGVGIAKENLQHIFERLYKCDKGRSKKGSGL
                     GLSIVSQMVERMGGKISVESEIGKYTVFIVSLPLI"
     gene            4729..5652
                     /locus_tag="L3M60_003682"
     CDS             4729..5652
                     /locus_tag="L3M60_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013273760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6228267.1"
                     /translation="MNNLIIETKNLTKQYGAQKSVSNLNIHVQKGRIYGLLGRNGSGK
                     TTTMKMLLGLTKPTTGEIKIFGQDIHNNEKKLLPRIGCLIESPGFYPNLTGTENLKIF
                     ARLRGIPKKNAVESALDVVGLPNNDKKLFSQYSLGMKQRLAIALAIMHDPQLLILDEP
                     INGLDPIGIAEVRKFIRNLCTERGKTILISSHILSEIALLADDIGIIDRGILLEEESL
                     AQLEEKNSKYIHFAVSDAAQAARLLEIQLNLKNFKVADNHNIMLYDTDVSVAEINRAF
                     IQNNIDVSESHLCEDTLEDYFKKVTGGEGIA"
     gene            5636..6406
                     /locus_tag="L3M60_003683"
     CDS             5636..6406
                     /locus_tag="L3M60_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228268.1"
                     /translation="MKELLDIISCEFSKLKRLKFILISILGACLFPIPATILIAKDNL
                     PFEQLFKLVVNFGYFLLLPIVLSIVASQLFFIERDNDVLKNLATVPVPKGKLALAKLA
                     VLLFISLFYSVAGLGATIIGGFIVGIVEGVAVKLGMSIVFGIMFFVTALPVVVLIVYF
                     NRSYIFSIILSFVYAIFNFSISLNIINFEPNNPLLSVLPAPVIMRWWMAFWGDPTGEY
                     TALRQPYLLSTPACVGILCLITIIAVLLICIIYKKQEN"
     gene            6416..7198
                     /locus_tag="L3M60_003684"
     CDS             6416..7198
                     /locus_tag="L3M60_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228269.1"
                     /translation="MLDIITTEFQKIKRYNILWIGVVAVLFSALLAVFQIVSSHGSDP
                     LTYESFANGVIWNNFSLSFPFVITLIGGYLINREYTDQTLKNILTIPISLRKLLVGKL
                     IAVGGITVLLSIFSFLCTFILGLLFCNINISVILVAKSLVQILSVNLYCYVAVLPIIA
                     YFGRKQNSFLTGVGIAFVYGFCGVFVAGRNLTDFYPITAGLGLAGYNNDDGSVYQPLI
                     GVMTLVVILILTAILLTFTPNYDKVMTISKKKGIKHKKYSHK"
     gene            7576..8112
                     /locus_tag="L3M60_003685"
     CDS             7576..8112
                     /locus_tag="L3M60_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HBN6228270.1"
                     /translation="MNLKGTKTEKNLMAGFAGESEARNKYTYYASKARKEGYNQIAAI
                     FEETANNEKEHAKLWFKLLHDGMPSTEENLKDAAAGENYEWTDMYAKFAKEAREEGFD
                     KIAYLFEAVGKIEKEHEERYLKLLENLNEGKIFKRDEEVVWQCQNCGHVYVGTEAPEK
                     CPVCDHPKAYFNIKAENY"
     gene            8144..8533
                     /locus_tag="L3M60_003686"
     CDS             8144..8533
                     /locus_tag="L3M60_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009905140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HBN6228271.1"
                     /translation="MKFSKQRELILNEILNNPVHPTADYLYENLKKDNPNLSLGTVYR
                     NLSQLTEHGFIKKVSIPGYPDRFDGRIDNHYHIICEVCGEVYDLESEVLNNLKELISE
                     ETDIKITSYNISFKGICNNCKRCSQVG"
     gene            8570..8956
                     /locus_tag="L3M60_003687"
     CDS             8570..8956
                     /locus_tag="L3M60_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004453642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HBN6228272.1"
                     /translation="MCSEQKFFICETCGNLVGMIQSGGVPIFCCGKPMKELVPNTTDA
                     AVEKHVPVIEVDGNSVTVKVSSAIHPMTKEHHIAWVYLMTEQGGQRKCLAVDGEPVVK
                     FALNDDDKVISAYAYCNLHGLWKAEL"
     gene            9083..10525
                     /locus_tag="L3M60_003688"
     CDS             9083..10525
                     /locus_tag="L3M60_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HBN6228273.1"
                     /translation="MSIYKCSVCGYIYDESKNDKTWDELSEDWECPVCTKGRSYFGKI
                     STVYYEEDENIAEDIVEDEPKLNTEKDGDLNYVSTYLRRDDEVEKHMDIIHEMAVTGK
                     SIIEPMRTKLPVISWDDILIMGAQLNPLPLNEHDEVNTTTIIGKKAKKPMIIENPVYI
                     SHMSFGALSKELKIALAKGAAKSKTAMCSGEGGILPEEKEASYKYIFEYVPNKYSVTE
                     ENLKNSDAIEIKIGQGTKPGMGGHLPGEKVTEEIAKVRNKPVGKDVISPSCFEEIQSK
                     EDLKKLVDELREVSEGRPIGVKISAGHIEKDMEFIAYAKPDFVTIDGRGGATGASPKL
                     LKDATSIPTIFALYRARKYIDTHGLDIDLVITGGLRISTDFAKAIAMGADAVAIASSA
                     LMAAACQQYRICGSGKCPVGVATQDEELRKRLHIENSANRVANFLNVSLEELKTFARI
                     SGHKDIHDLSVDDLYTVNSEISNYTNIQHV"
     gene            10822..10941
                     /locus_tag="L3M60_003689"
                     /pseudo
     CDS             10822..10941
                     /locus_tag="L3M60_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574155.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
BASE COUNT         3939 a         1389 c         2081 g         3566 t
ORIGIN      
        1 ggagaaatat ttatgaatac aggatttcca aaagattttt tatggggagc atcatcttct
       61 gcatttcaag tggaaggagc ttgggataaa gataataaag gaaaaaccgt agctgactat
      121 aattctttta aaaagtctca tttacaggca gatactaaag tggcatcaga cttttacaat
      181 aattatgaag aagatattga attaatgaaa gaattaggga tgaaaacata tcgtttttca
      241 atttcatggg caagaataat accagatggt gaaggagaga taaaccagaa aggtatagat
      301 ttttataata agataataga caaactaata gagtgtgata ttgaaccatt cgtaacttta
      361 taccattttg accttccatt tacattagta gagaaatata atggctggga aagtagagaa
      421 actgtctatg cttttgaaag atttgctaaa atatgcttta agcattttgg agatagagta
      481 aagtattggc aacctcataa tgaacagaat ttaattgtta gagtggaaga gagaattaat
      541 atatatgatg aaacagattc atggaaaata gataaaatgc gtgctcaaat ggactataat
      601 ctttgtttag cacatgcatt agcagtcaat gcatgccatg agatgataaa agaaagtaaa
      661 attggagcag cagtatcatc atctgtaaca tatcctctta cttccaagcc agaagatgta
      721 tatgcagcta gaatgaatga taattttaaa gtgtactata tgcttgatat gcatcactat
      781 ggagaatatc cagggtatta tatgaggtat cttgaaaaaa gaaatatagt ccctcatatg
      841 gaagatgggg ataaagaagt acttaaaaag gctaaaatgg attttatagc tgtaaattat
      901 tatagaacaa attgtgcagg agccttccca gaggattcac aacatccatt tgggttaaga
      961 gaaggaactg ttgattttag tatgtatgga ttatttaaaa tgtcaatgaa tcctaaccta
     1021 gaagcttctg agtatggagc agcaatagac ccatcaggtc ttagagttgc cttaaatgaa
     1081 tattggcaaa gatatcactt acctgttata ataacagaaa atggtcttgg agctaaagac
     1141 atattagaag atggaaaaat tcatgatgac tatcgtatag attatttaag gagtcatata
     1201 aatgcatgta gactagctat tgaagatggt gtagagatga ttggatattg tccatggtca
     1261 tttacagacc ttttaagctc ttctcaaggt tttaataagc gttatggact tgtttatata
     1321 aatagaactg accatgaagt tttagatttg aaaagaatca agaaagatag tttttactgg
     1381 tacaaagaag ttatagagaa caatggaata gtaaaataaa cataaatata tattgagggt
     1441 tttagtatgt acattacaaa ggtattaaat aattcgttgc tacttgcaag ggatgataat
     1501 gatgaagaaa taatattgat gggaaaaggg attggccata attataaggc tgggtctgag
     1561 ttaaataaaa aagatataga aaagattttt gttcttcatg atgaaaatat aaaaaagagt
     1621 attatacaac tggcaacaga aatagatgca gaatattttg gtatagctca aatgataatt
     1681 gcatatggaa tagaaaaata taacttaaaa cttatgaacc atatatattt ggccttaact
     1741 gaccacattg cctttgcagt gagacgcttt aaagaaggta taatgattga aaatcactat
     1801 ttgtttgaaa tcaaggagtt taatccaaaa gagtatgata taggaaaata tgcaattact
     1861 gtttttaagg aggttcttgg tttagaacta ccagaagaag agattggtaa tatagcaggc
     1921 cattttataa atgcacaaca agataatcct tatagtgata gaaataagag aagtgctaaa
     1981 attgtaaatg ctattttgca aatagttcat tatcactttt ctattgtata taataaagag
     2041 tcattttact ataggagatt tgtaatgcat ctaaaggcct tttcacaaag atttctggct
     2101 aacgaaccgt caaaagaaaa aatagatttt atatatgagc aagttcaaaa aaattgtaag
     2161 gaagagtatg aatgtgtgaa aaaggtagga gcttatatgt ataaggaata ctccagagaa
     2221 ttaccaagac aagaggagtt atatttgatg attcatattc ataagatttt aggagaatta
     2281 tgcgaaaata gttagaatat cttaatatgc tagtaatatt ataaaaaatt aaaaattaat
     2341 tacactttaa atatgaataa ttgcattact taagggggtt gtcttaaaat tgattttttt
     2401 aattaaattt taagttcaac tcctttttaa acctttatat cattacaact ttaatgttta
     2461 tacgcctgta cttcagatgt gtatctattt tttacatttc tcaacatgaa aatttatcat
     2521 tttttgtagt gtgttaattg aaataaacct ttgagttaac cctaaattta ttctggacct
     2581 acaggaatag ttccaaagat actttaaaat attttctgga atgattgctt atttcatata
     2641 tattttattg tgatgttttg atttaataaa ttcttcaact ccgtatatag taaatgtatt
     2701 atcatgtttg ttttatttaa gcacacttat aatatgcttg attttttcaa aatgatgaaa
     2761 tatactaatg ggtgttggat gtataagaat cacattatac ttgaaaaagt aagtgagaaa
     2821 tgaggtataa ttcctcatat gataaactac tattgtattg tggaacatat tctcagtgat
     2881 gtcaagatat tacaatatga ctaaattcct aatgataaaa aagtctacga atgagtgtag
     2941 aaaggggaac taagatgaat aaagttttaa ttatagatga tgataaagat ctttgtattt
     3001 tgttaaaaaa gagtatctca attgaacata ttgaagcaaa ctattgccat tctgggagag
     3061 atggattggt tgaactttca aaacaagatt atcagttagt agttctagac gtgatgatgc
     3121 caggtatgga tggttttgaa actatggaac aaatacgaaa atatagcagt attcccatct
     3181 taatgctgac atcgaaaaat gataattaca gcaaagttca tggattgcga gctggtgcgg
     3241 atgactatct aaccaaacca tttgaaattg cagaatttac tgctcgtgta ctgtctttaa
     3301 tccgacgtta cacccgtttt aatccaaaga atgaggagtc acagcaactt tctttcaaag
     3361 gtctgttgat tgaccttgac cagcatacag tgactaccaa tggcgaaact atgttgcttc
     3421 cacccaagga atttggtgtg cttctttact gtgcaaggca tcagggtaag attttgacaa
     3481 aacagcagat ttatgaagct gtgtggggtg agccatatgt atatgatgac agcaatatca
     3541 tggctattat cagtcgccta agaaaaaaaa ttgaactaga cccatcatct cccatctatt
     3601 tgcaaacgat aaaaggaatc gggtatcaat tcaacacgga ggtatgaaaa tggatgaaca
     3661 aacgataatt ctttttttgt ttttgctgtc gattggtcta cttttccttt ccatatttgc
     3721 tttttccaag cttggtcata ttcacaaacg tctaaaagat atagaagaaa tacttgctga
     3781 tgtacaaatt ggaaacgaaa atcgaaaaat actaattaag ccatgtgatg ttatggctcc
     3841 attggtttat cagcttaatg agattgtcta cgattatgaa aataagttac tttctctgaa
     3901 aaagtcggat aaagctagca aacaacttat gacaagttta tctcatgatg ttcgtacacc
     3961 attaacgact ctgattggtt atctggatgc tgtacacagt ggtatagtta tcggacaaga
     4021 acgggaggaa tacattgaaa tcgctcggcg tagagcctac gaccttaagg attatatcga
     4081 tgttcttttt gactggtttc gattgaactc tgatgaattt acgttgtcaa tagaatctgt
     4141 ggaaattgca gagttgtcac gtaatattct taaagattgg attcctatct tccatgaaaa
     4201 gaaattggat tttgaaattg atattccaga aaatcgacta atggtaaacc ttgattcaga
     4261 tggatattct cgtgtagtca ataatctagt tcaaaacgtt cttgcccata gcaaggctcg
     4321 ccgaatcaaa attaccatga gtgaggattc taagatggta ttacttcgtg ttgaggataa
     4381 tggtgttggg attgccaaag aaaatttaca gcatattttt gagcgactgt acaagtgtga
     4441 taaaggacgt tcaaaaaaag gaagtggtct tggtttatcc attgtcagtc agatggtgga
     4501 gcgaatgggc ggtaagattt ctgtagaaag tgaaattgga aagtataccg tatttatagt
     4561 gagcttacca ttaatataaa aattgttttt cccccatgtg atatatatac acagggggga
     4621 ttttttataa gctataaagt tgcaaggtta atacaaggtt gttgcaaggt taatgtaaga
     4681 ttgaggtggt atgataaatt taataaacaa aggaggattt tactatttat gaataattta
     4741 attattgaaa caaaaaatct cactaaacaa tatggtgctc aaaagagcgt ttctaatttg
     4801 aatattcatg ttcagaaagg tcgtatttat ggattgctgg gaagaaatgg ttcgggtaag
     4861 acgacaacta tgaaaatgtt attgggtcta acaaaaccaa ctactggaga aattaaaata
     4921 tttggtcagg atattcataa taacgagaaa aagcttcttc ctagaattgg atgtttgatt
     4981 gaatcaccag gtttttatcc taatctgaca ggaacagaaa atctcaaaat ctttgcgagg
     5041 cttagaggaa taccaaaaaa aaatgcagta gaatcagcac tggatgtagt cggtttgcca
     5101 aacaatgaca aaaagttatt ttctcaatac tctcttggta tgaaacagag gttggcaatt
     5161 gctcttgcca ttatgcatga cccacaattg ttgattttgg acgagcctat caatggactt
     5221 gatcctattg gtattgcaga agtaagaaag tttattcgta atctctgcac agaacgaggt
     5281 aaaactattt tgatttccag tcacattctt tctgaaattg ctttgttggc agatgacatt
     5341 ggcattattg accgtggcat tttgttggag gaagaaagcc ttgcacaact ggaagaaaaa
     5401 aacagtaaat acatacattt tgctgtatct gatgcagcac aagcagcaag acttctggaa
     5461 atccagctta acctgaaaaa tttcaaagtg gcagacaatc acaatattat gctttatgat
     5521 acagatgtct ctgttgcaga aataaatcgt gctttcattc aaaacaatat tgatgtatca
     5581 gaatctcatt tgtgcgaaga tactcttgaa gattacttca agaaagtaac agggggtgaa
     5641 ggaattgctt gatataattt catgcgaatt ttcaaaactc aagcggttaa aatttatact
     5701 gatttctatt ttaggtgctt gtttatttcc aattcccgca acaattttga ttgcaaagga
     5761 caacttgcct tttgagcaat tgttcaaatt agttgtcaat tttggatatt ttttgctatt
     5821 accaattgta ttgagtattg ttgctagcca actctttttc atagaaagag ataacgatgt
     5881 attaaaaaat ctagcaacag tgccagtgcc aaaaggaaaa ctggctctgg caaaattagc
     5941 tgtgttgctg tttatatcat tgttctactc cgttgcaggg ttaggagcta ctatcatagg
     6001 aggtttcatt gttggaatag tagagggggt tgctgtaaaa ttaggaatga gtattgtttt
     6061 tggaattatg ttttttgtta ctgcacttcc tgttgtggta ctcattgtat atttcaatcg
     6121 cagctatatt ttttctatta tactttcttt tgtatatgct attttcaatt ttagtatatc
     6181 cttaaatatt atcaactttg aaccaaataa cccactgtta agcgtattac ctgctcctgt
     6241 tataatgcgt tggtggatgg cattttgggg agatcccact ggcgaataca cggcacttag
     6301 acagccatat ttactctcaa ctcctgcttg tgtaggtatt ttatgtttga taacaattat
     6361 tgcagtcctg ttgatttgta taatctacaa gaagcaggag aattaggagg tatctatgct
     6421 tgatataatt actacagaat ttcaaaaaat aaaaagatat aatattctat ggattggtgt
     6481 tgttgctgtt ctgttttcag cactattggc ggtttttcag attgtaagtt cacacggcag
     6541 tgaccctcta acgtatgaga gctttgcaaa tggagttata tggaataatt tcagtcttag
     6601 ttttccattt gtgattacgc tcattggagg atatcttatt aaccgtgaat atacagacca
     6661 aaccttaaaa aacatcctaa ctattcctat ttcattacgc aaattgctag tgggaaaatt
     6721 aattgcggtt ggaggaatta ctgtattgtt aagtattttc agcttcttat gcacatttat
     6781 tttagggtta ctattttgca atatcaatat atctgttata ctcgttgcaa aatccttagt
     6841 tcagattttg tctgttaatc tgtattgtta tgttgctgtt ttgccgatta ttgcttattt
     6901 tggtcgtaaa caaaattcct ttttgacagg cgtagggatt gcatttgtat atggcttctg
     6961 tggtgtattt gttgctggaa gaaatctaac agacttttat ccaatcacag caggtcttgg
     7021 tctagctgga tacaacaatg atgatggtag tgtttatcag ccacttatag gtgttatgac
     7081 tttggttgta attctaatcc tgacagctat tctacttaca tttacaccta attacgataa
     7141 agttatgacc atatctaaga aaaaagggat aaagcataaa aagtattctc ataaataaca
     7201 taaatattgt ttctattatt taagttgtca ggcagtgaaa agaaaaaatc tgtcgtacag
     7261 tggctatatg ctcatgtatt taagaagcta cagttttgat ttttcatagg tgtggcttct
     7321 ttttgtattt attaaatcaa caactatcat aatatttgca ataggtatag taacaagaga
     7381 taatactgtc atatgtgttt tatatattac attaagtata ttctaaaata tatgttttaa
     7441 ataatatatt tgaaaataag aatacttgaa taaaaaaaat aatattatat atagttgaca
     7501 aaaagtggaa attaatatac tatataaata tagaaatagt aatcattact aatattaaaa
     7561 aaggaggaat taattatgaa cttaaaagga actaaaacag aaaagaattt aatggcagga
     7621 tttgcaggag agtcagaagc aagaaataaa tatacatact acgcttcaaa agctagaaaa
     7681 gaaggttata atcaaatagc tgctatattt gaagaaactg ctaacaatga aaaggaacat
     7741 gctaaattat ggtttaaact tttacatgat ggtatgcctt caacagagga aaatttaaag
     7801 gatgcagcag caggcgaaaa ctatgagtgg actgacatgt atgcgaaatt tgctaaagaa
     7861 gctagagaag aaggatttga caagatagct tatttatttg aggctgttgg aaaaatagag
     7921 aaagagcatg aagaaagata cttaaagctt ttagaaaact taaatgaagg aaaaatattt
     7981 aaaagagatg aagaagttgt ttggcaatgt caaaactgtg gtcatgtgta tgtaggtact
     8041 gaagcaccag aaaaatgtcc agtatgtgac catccaaaag catatttcaa tataaaagct
     8101 gaaaattatt aaaaattatc attattaaaa aggaatgtta actatgaaat tttctaaaca
     8161 acgagaactg attttgaatg aaatattaaa taatccggtt catcctactg cggattacct
     8221 atatgaaaac ttaaaaaaag ataatccaaa tctaagctta ggaactgtgt atagaaattt
     8281 atctcaacta acggaacatg gctttataaa aaaggttagt attccagggt atccagatag
     8341 atttgatggt agaatagata atcactatca tatcatatgt gaagtatgtg gagaggtata
     8401 cgatttagaa tctgaagttc ttaataattt aaaagaatta atatctgagg agacagacat
     8461 aaaaataaca tcttataaca taagttttaa agggatttgt aataattgta aaagatgtag
     8521 ccaagtaggt tagcttttaa attctaaaaa taattaatga ggtgagttta tgtgtagcga
     8581 acaaaaattt tttatatgtg agacttgtgg aaatcttgta ggaatgatac aaagtggtgg
     8641 tgtacctatt ttctgttgtg gaaaacctat gaaggagctt gttcctaata caacagatgc
     8701 agcagttgaa aaacatgtac ctgtgattga agtggatgga aatagtgtta ctgttaaagt
     8761 aagtagtgca attcatccaa tgactaaaga acatcatata gcatgggtgt atcttatgac
     8821 tgaacaaggt ggtcaacgca aatgtctagc agtagatgga gaacctgtag ttaaatttgc
     8881 tctgaatgat gatgataaag tgatttctgc atatgcttat tgtaatttac atggtttatg
     8941 gaaggctgaa ctgtaatatt taaataaatt gagtcaaaaa ggaggctata aatgtatttt
     9001 catacaaata gcctcctata aatttatata aattcgagtt ttaattttat ttaatgtcaa
     9061 tataatataa ggagaaaata atatgtcaat ttacaaatgt agcgtttgcg gatatattta
     9121 tgatgaatct aagaatgata aaacatggga tgaattaagt gaagactggg aatgccctgt
     9181 atgtacaaaa ggtcgtagtt attttggaaa gataagtact gtttactatg aagaagatga
     9241 aaatatagca gaagacatag ttgaagatga acctaaatta aatactgaaa aagatggaga
     9301 tttaaattac gtaagtacat atttaagaag agatgatgaa gttgagaaac atatggatat
     9361 aattcacgaa atggctgtta caggaaaatc tataatagaa ccgatgagaa ctaaactacc
     9421 agtaatttct tgggatgata ttttaattat gggtgctcaa ttaaatccat tacctttaaa
     9481 cgaacatgat gaagtaaata caacaacaat aatagggaaa aaagcaaaga agcctatgat
     9541 tatagaaaat ccagtttata tttcccacat gtcatttgga gctttatcta aagaactaaa
     9601 aatagcttta gcaaagggtg ccgctaaaag taagacagct atgtgtagtg gagaaggtgg
     9661 tatattacca gaagaaaaag aagcttcata taagtatatt tttgaatatg taccaaataa
     9721 atatagtgta actgaagaaa atcttaaaaa ttctgatgct atagaaataa aaattggtca
     9781 aggaacaaaa cctggtatgg gaggtcatct tcctggtgaa aaagtaactg aagaaatagc
     9841 aaaggtaaga aataagcctg taggtaaaga tgtaataagt ccatcttgtt ttgaggaaat
     9901 acaatccaaa gaagatttaa agaaacttgt agatgaactt agagaagttt cagaaggtcg
     9961 tccaataggt gttaaaattt cagctggtca tattgaaaaa gatatggaat ttatagctta
    10021 tgctaaacct gattttgtga ctattgatgg tcgtggtgga gctactggag caagcccaaa
    10081 gttgcttaaa gatgcaactt caatcccaac aatatttgca ctttatcgtg ctagaaaata
    10141 catagataca catggattag atatagattt ggttataaca ggtgggcttc gtatatctac
    10201 tgattttgcc aaggcaattg ctatgggtgc tgatgcagtt gctattgcaa gttctgctct
    10261 tatggcagca gcatgtcaac aatatcgtat atgtggttct ggtaagtgcc ctgtaggagt
    10321 agctactcag gatgaggaat tgagaaaaag attacatata gaaaatagtg ctaatagagt
    10381 ggcaaacttt ttaaatgtaa gtttagaaga acttaagact tttgctagaa tttctggtca
    10441 taaagatata catgatttaa gtgttgatga tttatatact gttaactctg aaatatcaaa
    10501 ttacacaaac atacaacatg tataagttga agtttgagac tattagattg tagatagtta
    10561 taaaagtaaa tgataaatat attttataaa aaggagtgtc ttaaaatgaa ctttttagtt
    10621 tataagtcac tcttttttgt atgaagccaa atatatttat cattttaaca atgaaaaaga
    10681 atcttatatc tttactttga gatagattcc ttctatttat gaaaattaaa gatattaaat
    10741 tgtaaaacat tctgtgatgt cgtactatta aactaaagat gctgtacttt aaagtaaata
    10801 aaaatttaag gagtgattat tatggaaaaa ttgaagattc aagttctagt tgacaataat
    10861 acatatattg ataggtactt tgttggagaa ccagcagtaa gctattacat tgagattgat
    10921 ggaaatagga ttttgtttga ttaaaaaata gctacgaaca cttgtgactt tagtc
//
LOCUS       DAFNNB010000378        10807 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0134, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000378 DAFNNB010000000
VERSION     DAFNNB010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 10807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10807
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0134"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            21..734
                     /locus_tag="L3M60_003690"
     CDS             21..734
                     /locus_tag="L3M60_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6228274.1"
                     /translation="MDTYFNKKILLVDDEKDIVDLIEEVLINDGFKNIIKAYNGLDAI
                     SLCKIACPDVVILDIMLPDIDGIEVCKKIREFSYCSILFLSSKNDDIDKILGLSSGGD
                     DYITKPFSPREIVFRVKAQLRRQQYQSIVPSDSSVIKIGDITIDIEGNRVYKDRKEIE
                     LTGREYHLLSYMAQNVNKIIGKERLYEQVWGVYSSICDNTIMVHIRHIREKIEDNPSN
                     PKILITVKGLGYKLVNRID"
     gene            complement(1011..2549)
                     /locus_tag="L3M60_003691"
     CDS             complement(1011..2549)
                     /locus_tag="L3M60_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HBN6228275.1"
                     /translation="MSLLKVTNLSQCFMDKSLYEKANFDLFKGEHIGVVGQNGTGKST
                     LIKILLGEVIPDSGEIKWQPNINIGHLDQYAEINRDTTISLYLHTAFEELYKIEKEMN
                     LLYQESAISGNEQHLIKASDYQEQLIANNFYSIDNEVNKIANGLGLDSIGMNRVVGEL
                     SGGQRAKVILAKLLLSNHEVLLLDEPTNFLDKEHVEWLSNYLNTFSGAFIVVSHDFDF
                     LEKISTGILDIEFGMIKKYHGKYSEFLKQKLHLREDYIRRYQSQQKKIEKEEAFIRKN
                     KAGVNSKIARGRQKQLDKIERIAPPGFTGKPNIKFSEIEISAQNALTVTNLEVGYYYS
                     LLPKLNFSVDGGQKIVITGFNGIGKSTLLKTLVKDIPCISGDFQFSEQVKLAYYEQDL
                     KWENPTKTPLQIVADKYPKLNTKEIRRHLARCGVKEEHVSRSVSTLSGGEQSKVKLCC
                     MMLFPCNFLILDEPTNHFDAETKDALQNALKQFRGSIILVSHEEKFYKGWIDKVFNIE
                     KQLV"
     gene            3557..3843
                     /locus_tag="L3M60_003692"
                     /pseudo
     CDS             3557..3843
                     /locus_tag="L3M60_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
     gene            3831..4157
                     /locus_tag="L3M60_003693"
     CDS             3831..4157
                     /locus_tag="L3M60_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HBN6228276.1"
                     /translation="MSSYLNLIREFNLKEKIIAFICVSALLIGKSCVLKNRKATTYHL
                     KNSKRQRQLSEFDVNVVNEPTVVNQNIITSYYTETAPHVAFKLLEMLISKKQLDEVKL
                     AMGFKL"
     gene            complement(4162..4485)
                     /locus_tag="L3M60_003694"
     CDS             complement(4162..4485)
                     /locus_tag="L3M60_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228277.1"
                     /translation="MKKCLDNYSCPIEATLALIGGKYKTLILWHLKDTILRFNELKKL
                     IPKATPKMLTQQLRELESDGLIIRVVYPVVPPKVEYSLSDFGKSIIPILDSMCDWGSD
                     YLEKL"
     gene            4637..5155
                     /locus_tag="L3M60_003695"
     CDS             4637..5155
                     /locus_tag="L3M60_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003429152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6228278.1"
                     /translation="MNFVELAKKRYSCRNYQDRKVEKEKLDKVLDVARIAPTGGNRQP
                     QRLIVIQEKEGLNKLSKAANIYDAPLAILVCGDKEKVWTRPFDGKQLTDIDTSIVTDH
                     MMLQATELGLASVWVCYFNPDIIREEFSLPDNLEPINILLMGYESKVADSPDRHEKTR
                     VPLSEIVSYEIL"
     gene            complement(5346..6014)
                     /locus_tag="L3M60_003696"
     CDS             complement(5346..6014)
                     /locus_tag="L3M60_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBN6228279.1"
                     /translation="MLEISHKVKEIYPNIKFGVMVINITHSKPNKENFLILKNSTIKN
                     IIEKHPEYNRKEKIKTEPVSSYIKYYKKFKKTYPVLLQLESILLKSKGIPDVGVTIES
                     MFLAELKNLLLTAGHDLDKIELPLKIDLANGSEHFYGIGAKEQILTKDDLFLSDNIGI
                     LSSILNGPDNRTAITKDTKNIMYFVYGPDKISEKQIQDHLNDIKYYISSAFPDLKINS
                     IDIF"
     gene            6129..7544
                     /locus_tag="L3M60_003697"
     CDS             6129..7544
                     /locus_tag="L3M60_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016729357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBN6228280.1"
                     /translation="MWITINRNSNISLSRQIYCEIKDMILNGSLKFEQKLPSSRTLSK
                     ELSVSRNTVLDAYNQLIAEGYLETRNGYGTIVAKGISGHKVDFKLNYIFRDEKDLHTN
                     KQKNYIDFRSGVPELKLFPRKELSKLYKQILYDLSDSDFRYGSTAGVWVLRAEISKYL
                     FRTRGIRCNPKNIMIVSGSTQGLSLISQLLYKSNKKVAVEDPIHKGLLKVISSIGYSV
                     VGVKVDDKGINTNLIKSIKGISFVYTTPSHQYPLGGILPIQRRLELIKYATENGYYII
                     EDDYDSEFRYEGQPISSLYELNPNKVIYIGSFSKILSPAIRVGFILLPDNILESYKTL
                     KMYSDVHTETILQHVLAEFIGNGGLEKYIFRMKKLYNRKRKYLIKVLNTEFKNEFKIK
                     GEAAGLHIVAHFYNVKFTESLIHKIFEAGIKVYPIQQYAIEKILEYDHEIILGYAHLS
                     FDEIEKGVKILSKIIHINQGT"
     gene            7767..8225
                     /locus_tag="L3M60_003698"
     CDS             7767..8225
                     /locus_tag="L3M60_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003436213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228281.1"
                     /translation="MEIIDYIEENLTENLNLDKIADIANYSKYHLHRMFTNIVGCTIH
                     QYIQRRRLTEAARQLIFTDKYIIDIAITAGYSTHQSFTLAFRNLYKESPQVYRNKDEF
                     YPIQQRFVLNLNHQIQSEKYISYANMNCKQRIDEINSYLNFNINCEVKVA"
     gene            8222..8806
                     /gene="clpP"
                     /locus_tag="L3M60_003699"
     CDS             8222..8806
                     /gene="clpP"
                     /locus_tag="L3M60_003699"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBN6228282.1"
                     /translation="MSLVPHVIEQTSQGERSYDIYSKLLKDRIIFIGEEINDAIASLV
                     VAQLLFLESEDPDSDIIIYINSPGGSVTAGFAIYDTMNYVRCDVSTICIGMAASMSAF
                     LLAGGTHGKRYALPNAEIMIHQPMGGAKGQATDVKIAVDNILKIKERLDRIFSENTGK
                     SIEDIRRDTDRDNFMTALEAKEYGLIDYIMNRNE"
     gene            9109..10185
                     /locus_tag="L3M60_003700"
     CDS             9109..10185
                     /locus_tag="L3M60_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBN6228283.1"
                     /translation="MITISLCMIVKNEEEVIGRCLECVKDIVDEIIIVDTGSTDSTKE
                     IVSKYTDMVYDFEWIDDFSAARNFSFSKASKDYTMWLDADDIILEADREKLLKIKESL
                     DTSIDIVMAKYNVSFDENGSPTLSYYRERLFKRSSNHKWVDPIHEVVPLSGEVFYSDI
                     AISHKKLHRQDPLRNLRIFEKMISEGKVLEPRHQFYYSRELYYNAKYKEAIEGFTKFL
                     DSSRGWIEDCISACRDLATCYYLINDEKSALYSLFRSFEFDEPRAEICCDIGKHMFDR
                     QKYKEAIFWYKVALTRDKNDTNGGFKSNDCYGYIPYIQLSVCYDRLGESNKAIYYHEK
                     TKEIKPNDSAVLHNENYFNKFKNS"
     gene            10252..>10807
                     /gene="bclA3"
                     /locus_tag="L3M60_003701"
     CDS             10252..>10807
                     /gene="bclA3"
                     /locus_tag="L3M60_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like exosporium glycoprotein BclA3"
                     /protein_id="HBN6228284.1"
                     /translation="MLLIMSRNKYFGPFDDNDYNNGYDKYDDCNSGRDDYNSCDCHHC
                     CPPSCVGPTGPMGPRGRTGPTGPTGPTGPGVGATGPTGPTGPTGPTGNTGGTGRGGPT
                     GPTGPTGPTGPTGPIGFGITGPTGATGATGATGADGVTGPTGPTGATGANGITGPTGA
                     TGATGFGVTGPTGPTGATGVGVTGA"
BASE COUNT         4018 a         1385 c         1704 g         3700 t
ORIGIN      
        1 ttttatggag agatttcaat atggatacat attttaataa aaaaatatta cttgttgatg
       61 atgaaaaaga tatagtagat ttaattgaag aagtacttat aaatgatggt tttaaaaaca
      121 ttattaaagc ttataatggt ttagatgcta tttcgctgtg taagatagca tgtccagatg
      181 ttgtcattct tgatataatg ttaccagata tagatggcat agaagtgtgt aaaaaaattc
      241 gagagttttc atactgttct attctttttc tctcatcaaa aaatgatgat attgacaaaa
      301 tactaggtct tagtagtggg ggagatgact atataacaaa gccattcagt ccaagagaaa
      361 ttgtttttcg agttaaagcg caattacgta gacaacagta tcaaagtatt gtaccatcag
      421 atagttcagt tattaagata ggagatatta ctattgatat agaaggaaac cgtgtatata
      481 aagatagaaa agagattgag ttaactggaa gggagtatca tcttttaagc tacatggctc
      541 aaaatgtaaa taaaattata ggaaaagaaa gactctatga acaagtatgg ggagtataca
      601 gtagtatttg tgacaataca attatggtgc atatacgaca tataagagaa aaaattgaag
      661 ataacccctc aaatcctaaa atacttataa cagttaaagg tctaggatat aaacttgtga
      721 atagaatcga ttagaataga tcttagtatc aatgaaggtg tctcaaattc aatttgttag
      781 ttaatgagac attctttttt gtgtgaaatc aaatgtattt ttgttatatc aataatgaag
      841 aagaggctta tctctatttt gagatagcct cattaaattt ataccgtggt aatgcacttt
      901 atgctctaat aaatatttat tttttagttt acttgcagtg aaacattaac taggagatta
      961 aaatttgcat tgaaatgcta attaaacaat atcgcatatt gtatataaac ctacactaat
     1021 tgtttttcta tattaaatac tttgtcaatc cagcctttat aaaatttttc ttcatgggaa
     1081 actagaatta tacttcctct aaactgtttc aaagcatttt gtaaagcatc ctttgtttct
     1141 gcatcaaagt ggttagttgg ctcatctaaa atcaaaaaat tacaagggaa taacatcata
     1201 caacataatt ttaccttaga ttgttcacca ccacttaatg tagatacact ccttgaaaca
     1261 tgttcttcct ttacgccaca tctggctaga tgacgtctaa tctcttttgt attcaatttc
     1321 ggatatttat ctgctactat ttgaagcggt gttttagtag gattttccca ttttaaatct
     1381 tgctcataat aagcaagttt cacttgttca gaaaactgga aatctccaga aatacaagga
     1441 atatctttaa ctaatgtttt taacaaagta gatttaccaa ttccattaaa acccgttata
     1501 actatttttt gacccccatc aacagagaaa tttaattttg gaagcaaaga atagtagtag
     1561 ccaacttcaa gatttgttac tgtaagagca ttttgagcag atatttcaat ttctgaaaat
     1621 tttatatttg gttttcctgt aaaaccagga ggagctattc tttctatttt atccaattgt
     1681 ttttgtcttc ctcttgcaat ttttgaattt actccagctt tgtttttacg tataaatgcc
     1741 tcttcttttt ctattttctt ttgttgtgat tgatatcttc taatataatc ctctcttaaa
     1801 tgtaattttt gtttaaggaa ctctgaatat tttccatgat atttcttaat cataccaaat
     1861 tctatatcaa gaatacctgt agaaattttc tctaaaaagt caaaatcgtg agaaactaca
     1921 ataaatgctc cactaaatgt atttaaataa tttgagagcc attcaacgtg ttctttatct
     1981 aaaaagtttg ttggttcgtc taataataaa acctcatggt tagaaagtag caattttgct
     2041 aaaattacct ttgccctttg cccaccgcta agctccccaa caacacgatt cattcctatt
     2101 gaatctaatc caagaccatt tgctatttta tttacttcat tatctataga atagaaatta
     2161 tttgctatta attgttcctg ataatctgat gctttgatta gatgttgctc atttccagat
     2221 atagcacttt cttgatagag taaattcatc tccttttcta ttttataaag ttcttcaaat
     2281 gcagtgtgta aatagagtga tattgttgta tctctattga tttctgcata ttggtctaaa
     2341 tggcctatat tgatattagg ttgccacttg atttctcctg aatctggaat aacttctccc
     2401 aataaaattt taattaatgt acttttgcct gtaccatttt ggccaactac acctatatgt
     2461 tcacctttga ataaatcaaa gttagctttc tcatacagtg atttatccat aaaacattgt
     2521 gataaattag ttacttttaa taaactcata attcaaactc cttttctttt attaaaaaat
     2581 ttatggaaat aaaaaaagca gaggaatgac attataaaat gccgacctct gcttttggaa
     2641 aaacaaaagg tcatagacaa gcattgttgt ctatgaccag aaaatctata ttatctatcg
     2701 gcaaataggt aattttaaat aagcatagcc caaaaactac actaaaccta tatttgccga
     2761 tgaagataga aagctttaag ctttcagatt aacctctaca caacgcttca tcgtatacat
     2821 ttgtgtagag ttaagttttc taacaagttt aactaattct atataaatca taattattca
     2881 catcctaatc ttagtattaa ttctagttta tctatattta aattgtactt ttgtactttg
     2941 tatattgcct tatttactta aaatatattt tattaattat agcttaaatc atgttatcat
     3001 aatacttagt tatagtcaat aagaaagtta tatttgctac ataacctatt aatttagtaa
     3061 atctatcatt acaaagttat ttgaagactc accaccaata aaattttata atctattaaa
     3121 aaattaagat tacgagattt ataattttga tacaggtata ctatctttat taaatattgc
     3181 aacatgtaga agtagatgga taatattatt aaattttcaa tgatatgcta atgtacatat
     3241 ataattatta gaaataaaaa tataatagta tgaaagtaac tgatataata actaaatgat
     3301 taaacatata gtaaaagtac ttcataaatt atggagtatc ttttattgtc gcaaaaataa
     3361 gcaatatatt atacaaaata aaaacattaa ttcaacttaa ataattttaa gtgtaatatg
     3421 tattttgatt aaataagcta aaattctaag aaaaaataat atatttgtat taagttttat
     3481 tgaaataatt aaaattattt acttaatgta aattgttagc ttaaattata ttaaataatt
     3541 aaggcggtga gtgggtctgg atgagataga tttaaaaata attaattctc taagaaagaa
     3601 tggtaggttt caacttctga aataagcaga aaaattaatt tatcaatacc agcagtctca
     3661 gagcatataa ggaaaattga gcaattaaat ttaattcaaa agtacactat tcaaattaat
     3721 caggaaaaaa taaaatataa gcttttggct tttgtattta tcaatacgga tttatctaaa
     3781 agtatggaag aatttaagaa aactatattg gaatatgata ctgcattaga atgtcatcat
     3841 atttaaatct aataagggag tttaatttaa aagaaaaaat aatcgctttt atttgtgtgt
     3901 cagcattgct gattggaaaa agttgtgttt taaaaaatcg taaagctact acatatcatt
     3961 tgaaaaatag taaaagacaa agacagttaa gtgagtttga tgtaaatgta gttaatgaac
     4021 caactgttgt aaatcaaaac attataacct catattatac agaaacagct ccacatgtag
     4081 cttttaagct tttagaaatg ttgatatcta aaaaacaact ggatgaagtt aaattagcta
     4141 tgggatttaa actgtaatag tttagagctt ttccaagtaa tcagaacccc agtcgcacat
     4201 ggaatctaaa attggaataa tactctttcc aaaatcagat agtgagtatt ctacttttgg
     4261 aggaacaaca ggataaacca ctcttatgat taaaccatct gattctaatt cacgtaattg
     4321 ttgagttaac attttaggtg ttgccttagg gattaatttt tttaattcat tgaacctcaa
     4381 tatagtgtct ttaagatgcc aaagtataag tgtcttatat ttaccaccaa tcaatgcaag
     4441 tgttgcttct attgggcaac tgtaattatc taaacatttt ttcatttttg gactccttta
     4501 tttattttaa agtatctttt taggtactat attacaaaaa agtgcattat tgtaatatat
     4561 taccatagtg ctaaaattat agcataggtt aaataaatca tcaacttagt ctaataaaac
     4621 aataggaggt aaataaatga atttcgttga acttgcaaaa aaaagatatt cttgccgtaa
     4681 ttatcaagat agaaaggttg aaaaagaaaa acttgataag gttttagatg ttgcaagaat
     4741 agctccaact ggtggtaatc gtcaacctca aagattgatt gttattcagg aaaaagaggg
     4801 tctaaataaa ctttccaagg ctgccaatat atatgatgct ccattagcaa ttcttgtgtg
     4861 tggagataaa gagaaggttt ggacaagacc ttttgatggg aagcaactta cagatattga
     4921 tacaagtata gttacagacc atatgatgct acaagcaact gaacttggat tggcaagtgt
     4981 ttgggtatgc tattttaatc cagatataat tagagaagag tttagtttgc cagataatct
     5041 agaaccaata aatattttat tgatgggata tgaatctaag gtagcagata gcccagatag
     5101 acatgaaaaa actcgtgttc ctttaagtga aattgtttct tatgaaattc tttaattata
     5161 aaattaagtt attcataggt taaatgtaac aaagtgttta ttataaataa aactcaaaat
     5221 aaataatatc cttagataaa taatatctat ataattaatt ttagtgaagt atatctagtt
     5281 taactagaat atatacttca ctaatttatt tgttcaaaat atttgtgagg attttaatat
     5341 attaactaaa atatatcaat tgaattaatt tttaaatctg gaaaagcact tgaaatatag
     5401 tattttatgt catttaaatg gtcttgaatt tgtttttctg atattttatc aggtccatat
     5461 acaaaataca taatattttt agtatctttt gttatagctg ttcgattatc aggtccatta
     5521 agaatgctag ataatatacc aatattatcc gataaaaata aatcatcttt tgtcaaaatc
     5581 tgttctttag cacctatacc ataaaaatgt tcacttccat ttgctaagtc tattttcaaa
     5641 ggtaattcta ttttatcaag gtcatgacct gctgttagaa gaagattttt caattctgct
     5701 aaaaacattg actcaattgt tacacctaca tctgggatac cttttgattt taatagaata
     5761 gattctaatt gaagcaatac agggtaagtt ttcttgaatt ttttgtaata ctttatataa
     5821 cttgaaacag gctctgtttt aattttttct ttacgattgt attctggatg tttttctata
     5881 atatttttaa ttgtagagtt tttaagaatt aaaaaatttt ctttattagg ttttgaatgt
     5941 gtaatattta taaccataac tccaaattta atattgggat aaatttcttt cactttatgt
     6001 gatatttcta acataatata gactcctttt tattttttgt tttatgatat aatcttagca
     6061 agttaaaagt agattttgta gttccatttt tatagtaatt ttatagaacc acttttggag
     6121 gtattagaat gtggattacg attaatagaa atagtaatat atcattatca agacaaattt
     6181 attgtgagat aaaagatatg attctgaatg gaagcttaaa gtttgagcaa aaattgccat
     6241 catctaggac tttatcaaaa gaactatctg tatctcgtaa tacagtttta gatgcatata
     6301 atcaattaat tgcagaagga tatttggaaa caaggaatgg atatggaaca attgtagcta
     6361 aaggaatatc agggcataaa gttgatttta agttaaatta tatttttaga gatgaaaaag
     6421 atttacatac taataaacaa aaaaattata tagattttcg ttcaggagtt ccagaattaa
     6481 aactatttcc acgcaaagaa ctttcaaagc tatataaaca aatactgtat gatttatctg
     6541 attcggattt cagatatgga agtacagctg gtgtatgggt tttaagagcg gaaatatcta
     6601 agtatttatt tcgtactaga ggaattagat gtaatcccaa aaacattatg attgtttctg
     6661 gttcaacaca aggtttatct ttaatttcac agcttttata caaaagtaat aaaaaggttg
     6721 ctgtagaaga ccctattcat aaaggcttat taaaggttat ttcatctatt ggatattcag
     6781 ttgttggagt taaagtagat gataagggaa taaataccaa cttaataaaa tcaataaagg
     6841 ggatttcttt tgtttatact acaccttctc atcaataccc attaggaggg attttaccta
     6901 ttcaaagaag actggagctt attaaatatg caacggagaa tggttactat ataattgaag
     6961 atgattatga tagtgaattt agatatgagg gtcaaccaat tagttctctt tatgaactta
     7021 acccaaataa agtaatttac ataggttctt ttagtaaaat attatctcca gccattcgtg
     7081 ttggatttat attattacca gacaatattt tagaaagcta taaaacctta aaaatgtact
     7141 ctgatgttca tacagaaaca atattacaac atgtattagc tgaatttatt ggaaatggag
     7201 gtttagagaa gtacatattt agaatgaaga agttgtataa tagaaaacgt aagtacctca
     7261 taaaagtttt aaacacggaa tttaaaaatg aatttaaaat aaaaggtgaa gcagcagggc
     7321 ttcatatagt tgctcatttc tataatgtta aatttacgga aagtttaatt cataaaattt
     7381 ttgaagcagg cataaaagtt tatcctatac aacaatatgc aattgagaaa atattagaat
     7441 atgaccatga aattatacta ggttatgctc atcttagttt tgatgaaatt gaaaaaggtg
     7501 taaaaatact cagcaaaata atacatataa atcaaggtac atagataaaa catatagcaa
     7561 tctacatata atagatgcaa tgaagtttaa actttaaaag aaatatatat taatattttt
     7621 gtataaaaat acctgatatt gtgttatcga tactttgttg ctatacacta aattaagata
     7681 gcaatttata ttaagaaaaa actttttgca taggttataa tacttaaaag gagaggatag
     7741 agttgaaaac aaatacagat gttattatgg agataattga ttatatagaa gaaaatctta
     7801 cagaaaatct aaatttagat aaaatagctg atattgctaa ttattcaaaa taccatttac
     7861 acagaatgtt tacaaacatt gtaggatgta ctattcatca atacatccag aggaggagac
     7921 tcactgaagc agctagacag cttatattta cagacaaata tattatagac atagctatta
     7981 cagcaggata ttctacacat caatctttta cactagcatt tagaaattta tataaagaat
     8041 ctccacaagt atatagaaat aaagatgaat tttatcctat tcaacagaga tttgttttaa
     8101 atctaaatca tcagattcaa agcgaaaaat atatttctta tgcaaatatg aattgtaaac
     8161 aaagaataga tgaaattaat tcttatctta actttaatat taattgtgag gtgaaagtag
     8221 catgagttta gtaccacatg taattgaaca aacaagtcaa ggggaaaggt cttatgatat
     8281 atattcaaaa ttattaaaag atagaattat atttatagga gaagaaatta atgatgctat
     8341 tgcaagttta gtagtagctc aattactgtt tttagaatca gaagacccag attcagatat
     8401 aattatatat ataaatagcc ctggtggctc tgttacagca gggtttgcta tatatgatac
     8461 tatgaattat gtcagatgtg atgtatcgac tatttgtatt ggtatggctg caagtatgag
     8521 tgctttttta cttgcaggag gaacacatgg aaaaagatat gctttaccta atgcagagat
     8581 tatgattcac cagccaatgg gaggagctaa aggtcaagca acagatgtaa aaatagctgt
     8641 agataatata ttgaaaataa aagaacgatt agatagaatt ttttctgaaa atacaggtaa
     8701 atcaatagaa gatattagaa gagatacaga tagagataat tttatgactg cactagaagc
     8761 taaagagtat ggactgattg attatataat gaataggaat gaataaagta tcatattaaa
     8821 atatctgttt tataaatata tatagaatat taaataagag tgtctcaaag tgaacttttt
     8881 agtttatggg atgctctttt ttgtatgaaa ttaaatatag ttttattatt tcaataacta
     8941 gtatcaaatt tcaagtttaa taaattctac cataaaaata cataaaagat aaggtttttt
     9001 agtaactttc tgtcgtcaaa agtttttata aatttaataa caaaacaaga gaaaatacca
     9061 tatattactt atgtctgcaa tatatagaag acaaaggagt tgtacattat gattacaata
     9121 agtttgtgca tgattgttaa aaatgaggaa gaagttatag gcagatgctt agaatgtgta
     9181 aaggatatag tagacgaaat tataattgta gatactggtt ctacagattc cacaaaagaa
     9241 atagtctcaa aatatacaga catggtgtac gatttcgaat ggatagatga tttctctgct
     9301 gcaagaaatt tttcattttc taaagcttct aaagactata cgatgtggct tgatgcagat
     9361 gacataattt tagaagctga tagagaaaaa ttattaaaga ttaaagaaag cttggatact
     9421 tccattgata ttgttatggc aaaatacaat gtatcctttg atgaaaatgg tagcccaact
     9481 ctctcgtatt atagagaacg attgtttaaa agaagttcta atcataaatg ggttgaccct
     9541 attcatgaag tagtacctct ttctggagag gtattttatt ctgacattgc aatatctcat
     9601 aaaaagcttc atagacaaga ccctctaaga aatcttagaa tttttgaaaa aatgatttca
     9661 gaaggaaaag tattggagcc aagacaccaa ttttattact ctagagagtt gtactataat
     9721 gctaagtata aagaagcaat agaaggtttt actaagtttt tagactcatc aagaggatgg
     9781 attgaggatt gtatcagtgc ttgcagagat ttagccacat gttattactt aattaatgat
     9841 gaaaagagtg ccttatattc tttgtttaga agttttgaat ttgatgagcc aagagcagaa
     9901 atttgttgtg acataggaaa gcatatgttt gatagacaaa aatataaaga agctattttt
     9961 tggtataagg tagcactaac aagagataag aatgatacta atggaggttt taagtcaaat
    10021 gattgttatg gatatatacc atatatacaa ttaagtgtct gttatgacag gctaggagaa
    10081 tcaaataaag ctatttatta tcatgaaaaa acaaaagaaa tcaaacctaa tgatagtgct
    10141 gttttgcaca atgaaaatta ttttaataaa tttaaaaata gttgataggt cacaataata
    10201 ctaaatttga catattatat atagagctta gaagctcaaa tatatcagga ggtgctttta
    10261 ataatgagta gaaataaata ttttggacca tttgatgata atgattacaa caatggctat
    10321 gataaatacg atgattgtaa cagtggtcgt gatgattata atagctgtga ttgtcatcat
    10381 tgctgtccgc catcatgtgt aggtccaaca ggtccaatgg gtccaagagg tagaacaggc
    10441 ccaacaggcc caacgggtcc aacaggacca ggagtaggtg caacgggtcc aacaggtcca
    10501 acaggcccaa caggtccaac aggaaataca ggaggaactg gaagaggagg cccaacaggt
    10561 ccaacaggtc cgacgggtcc aacaggtcca acaggcccaa taggatttgg aataacaggt
    10621 ccaacaggcg caactggagc aacaggtgca accggagcag atggagtaac aggcccaaca
    10681 ggcccaaccg gagcaaccgg agcaaatgga ataacaggtc caacaggtgc aaccggagca
    10741 acaggatttg gagtgacagg tccaacaggc ccaaccggag caacaggagt aggagtaaca
    10801 ggagcaa
//
LOCUS       DAFNNB010000379         9782 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0146, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000379 DAFNNB010000000
VERSION     DAFNNB010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9782
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0146"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            37..969
                     /gene="blaCDD"
                     /locus_tag="L3M60_003702"
     CDS             37..969
                     /gene="blaCDD"
                     /locus_tag="L3M60_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDD family class D beta-lactamase"
                     /protein_id="HBN6228285.1"
                     /translation="MKRKKNFIWTTILLVGIVMVMYYAGKKHNDINQKTDKNYIKSDL
                     NKSEINNDNNKKERVNIVNYSDCFEGISGGAIFYNSRNQEYNIYNKDLIETRASPCST
                     FKIISTLIGLEKGVITSKKSVIGYDGTNYPNKNWNKNLSLEEAFKESCVWYYKKLINK
                     VDAKSVQSILDDLKYGNCDISEWEGDLKNGKGHLNGFWLESSLQISPKEQVQTMVKIF
                     EGDTNFKKEHINILKDIMKIDVSDKNINVYGKTGTGFNGKNKRVDAWFVGMLESKDDT
                     YYFAIKSDDSNKEIAGPKVKEIAINIIKKYYSVM"
     gene            1318..2001
                     /locus_tag="L3M60_003703"
     CDS             1318..2001
                     /locus_tag="L3M60_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6228286.1"
                     /translation="MEILKCENLTKIYGSNQTRVTALNNVNLSVQKGDFVSIVGASGS
                     GKSTLLHMLGGVDRPTSGKIYVEDTEISSLNEEALAVFRRRKVGLIYQFYNLIPTLDV
                     RKNILLPMLLDKRKVDEDRFSEIVSILGLSDRLNHLPSQLSGGQQQRVSIARSLIYRP
                     TILLADEPTGNLDRKNSEEIVDLLNLSNKRFNQTILLITHDEKIALEANRIVTMEDGV
                     IVSEKVVKK"
     gene            1998..4658
                     /locus_tag="L3M60_003704"
     CDS             1998..4658
                     /locus_tag="L3M60_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBN6228287.1"
                     /translation="MNILQEYTLDFVRRNKRSSIAIMLAILLTTTMMSSLCGLLYTMW
                     TDFIRLSIEKEGNWHGELFDDTYGKDLSLIENFSSVDDVMVKGQWYVGKINNDKRDYI
                     VYRNATSEYWNHMPEKDSIIEGRRPSNTNEIALSKQYFENNPSVKIGDKITLPIGDRI
                     LNGKPLEPVAPKSKGESFKKISKTTLTIVGKVDVATSSVVPAYTALGYLDKTSIKPND
                     SITVYLRFKNIRDTYKELPKLAKSLGWKTNEYGKYNLKYNSNYLLKMLVFSPEQKASM
                     SNISKFSTPIMYLTIAIFTVAVFVMVIYNAFSLSANARLTQLGILSSVGASPKQIKRS
                     VVFEGFLLTIIPLPIGLFLGWLLCNKLIVYINSVNYHTDAPEVVFTYGIPAFLPAVLL
                     TIVTVWISTLIPARKVSKISPIEAIRQGDSVKIKKSHKYSLGKIFGIEGELASNALRA
                     RKKSYRTATISLTLSFLLLTGFLHIIANQEGAEAVYGSDNYKDQRDIEVYLENSEPVE
                     SDFIEKLDNTEGVKNSLYYQKMSATTWLTEKDASKELSKNGGFKSIVSSKKYSPIERD
                     GKFRIISNIIGLDDESFAEYCRQISVDPKLFYDTKNPTSIVYNKEEDVNRSTRRNKVY
                     IDYLNLSIGDNIKLNEKIYDEDKGDYTYNMKVGAITSTLPKIIDTSSRYTLMQIVPIS
                     MVQEISKNYAEVGRSRSNRLIGLFKVDNREDIPRVRGKLESICNKDYGSGDYSIEDVV
                     ENEKQNGSGRKTMNLIVGFITGLLAMIGLSNVLATVSGNIRSRRQEFAMLRSVGLSPD
                     GIKKMLVLEGLFLGITPLLLSIPVQIGIVYTFLRINEIYFIEYLPFAPISTIIGFTIL
                     ILFIVIASYMTGYNQLKNENIVESIKSETI"
     gene            complement(4835..6016)
                     /locus_tag="L3M60_003705"
     CDS             complement(4835..6016)
                     /locus_tag="L3M60_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016728749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HBN6228288.1"
                     /translation="MKTPRNINSTVVFIIFCLIVTFLLSVILTTITSFNEYNVLSKVL
                     DASYEYNPKSTESILKSLKNSSDEDFKTGNEILSKHGYSKGTFWSKNFFYFLVVSFGI
                     ISTMAIVVYIIKYRTNKQKLSRINMLTRYLEEVNLGKESVLLRYEDEFSYLEDEIYKT
                     VGELRCSKEFALKERQTLSDNLADIAHQLKTPITSMSLMAQLLSENCPDEEIDYINRL
                     TKQISRLERLVSSLLTLSKLDASTLIFESKLIDVHSLLTYAIEPIEGSLREKHQTFTI
                     KGSPSIEFEIDINWTLEALLNILKNCSEHIEAGGHIIAYYSQNPLYVEIIIEDNGKGF
                     AQEELPYIFNRFYRGKNASKDSIGIGLALSKSIIERQNGTLHAENCHNGGARFIIKFY
                     T"
     gene            complement(6016..6678)
                     /locus_tag="L3M60_003706"
     CDS             complement(6016..6678)
                     /locus_tag="L3M60_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009901935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6228289.1"
                     /translation="MAKILLLEDDDTITFGIKTSLENKNHIVCCYNTIAEAKKNFDKT
                     TDLILLDLNLPDGIGYEFCQYVKSIEDTPVIFLTVRDDEKDIIRGLDMGADDYVVKPF
                     LLSVLQSRINAVLRRAHSNTNNTITCGNISLNKVEVKAYLDSVEVPLTAGEYKLLLSL
                     MENKNRAMTRTKLLEMLWDVDGNFVNDNTLTVTIKRLREKLKNPPCIKTLRGIGYRME
                     DS"
     gene            complement(6794..7432)
                     /locus_tag="L3M60_003707"
     CDS             complement(6794..7432)
                     /locus_tag="L3M60_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBN6228290.1"
                     /translation="MSELSKRDKEKIQRENEIIDKAEKLFSLNGFDNTTMNELAKEVE
                     YTKRTIYKYFSCKEDLFFAVVLRGYKRLWDNVKIESAKGKTGFEKIKLSYFAFHKFYS
                     VEPSLLCLMGMIGIVNSKKSDTEMLFKEKFFSFNKFMFDEIQEMFEIGKNDGSIRYDI
                     EISTLMYSSIFTLTGFFNLLSVTRKSYLNNFNIDEEKFIETTLAILIDSIKA"
     gene            7612..8217
                     /locus_tag="L3M60_003708"
     CDS             7612..8217
                     /locus_tag="L3M60_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HBN6228291.1"
                     /translation="MKIKENVLRLDSTRGSNCFIVFDKEITLIDTGLPFMGKNIIKEL
                     KTLGIKLTDIKHILLTHHDVDHMSNIKLLKEKTGAKVWAHTEDIPFIIGEKDRPGFKK
                     FIGKTISRRLIKDVEPYGENMQIGNIKIIHTPGHTPGHVCMIFEDVLFAGDLVKNKNG
                     NIVSYPNPWNWDSKKMMKSIRELDNLEYEWICMSHGTPCIK"
     gene            8318..9040
                     /locus_tag="L3M60_003709"
     CDS             8318..9040
                     /locus_tag="L3M60_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HBN6228292.1"
                     /translation="MIRTLILYYLNIKPTHGYEIQKFLQVSGADRWTKIQSGSIYYAL
                     AKLEKDGGVRVLKEEKTGARIRKIYEITQSGKLELREEIQKELQMPIVPTGSNKFLLH
                     NILDVLPKDTLQKNLEKHIKYLIEQKKYWENWKEIKKIDKKSLATEKIAFDMTIDNLN
                     YQILWHEEILNDIDKYISVGCETQNIIKSIDFSNIEEDFLFTSDTTNELLEVQRLRDE
                     IINNPDKAIENIDKIILKLQNK"
     gene            9142..>9782
                     /locus_tag="L3M60_003710"
     CDS             9142..>9782
                     /locus_tag="L3M60_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003417478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBN6228293.1"
                     /translation="MSNILLEIKNLSKKYNNKEVVSIESFKLLEGDILGFIGANGAGK
                     STSINMLTTIVSPDSGSIYYKGKDIKNQQNIFKSSLGVVPQELAIYEDLSAYDNVKFF
                     CSLYGFKGNELKERTKDALEFVGLWESHKEFPSKFSGGMKRRLNIACAIAHSPKILIM
                     DEPTVGIDPQSRNKILEAIKCINTNGTTVIYTSHYMEEIETLCNRIALIDKGI"
BASE COUNT         3690 a         1207 c         1567 g         3318 t
ORIGIN      
        1 aggttataaa tttaaatatg ggagatgata taaattatga aaagaaaaaa gaattttatt
       61 tggacaacta tcttgttggt tggtatagtt atggtaatgt attatgcagg taaaaaacat
      121 aacgatataa atcaaaaaac tgataagaat tacataaaat ctgatttaaa taaatctgaa
      181 attaataatg ataacaataa aaaagaaaga gttaatatag ttaactatag tgattgtttt
      241 gaaggtatta gtggaggagc tattttttat aatagcagaa atcaagaata taatatatat
      301 aacaaagatt taattgaaac aagagcttca ccttgttcta cttttaaaat aatttcaaca
      361 ttaattggtt tagaaaaagg ggtaataacg tcaaaaaaat cagttattgg gtatgatggt
      421 acaaattatc caaataaaaa ttggaataaa aatcttagtt tagaagaggc atttaaggag
      481 tcttgtgttt ggtactacaa aaaattgata aataaggttg atgctaaatc tgtccaaagt
      541 attttagatg atttaaagta tgggaactgt gatattagtg aatgggaagg agatttaaaa
      601 aatggaaagg ggcatttaaa tggattttgg ttagaatctt cattacaaat atctccaaaa
      661 gaacaagtgc aaacaatggt gaaaatattt gagggagata caaactttaa aaaagaacat
      721 attaatattt taaaagatat tatgaaaata gatgtaagtg ataaaaatat aaatgtatat
      781 ggaaaaactg gaacaggatt taatggaaaa aataaacgtg tagatgcatg gtttgttggt
      841 atgctagaga gtaaagatga cacttattat tttgctatta aatcagatga ttccaataaa
      901 gaaattgcag gaccaaaggt aaaggaaatt gcaattaata taattaagaa atattatagt
      961 gtaatgtaat aaacttaatt ttatttgtaa atattggtta cctatagtct gtaaatattt
     1021 attaaataat tttactaaat tgagtttaca tagagcaaaa ctcctctagt atataaaata
     1081 cttgctattg attattaaat tgaatactta tagatattaa agttaatatc ttttaaaggc
     1141 tatgcaatat attaaaagta taaataaaat atattgcata gtttttgttt tgtattataa
     1201 ttttaaataa tgttcaaaaa ctaaacaata tttttaatag taatcgatat ttgtattcat
     1261 gtcactataa tgtcatttta ggttgctaaa atttaaccat aaaggagatg agtgtatatg
     1321 gaaatattaa aatgtgaaaa tctcacaaaa atatatggtt caaatcaaac cagagtgaca
     1381 gctcttaata atgtaaattt atcagttcaa aaaggtgatt ttgtatccat tgtaggagca
     1441 tctggttctg gcaaatctac attgctacat atgcttgggg gagtagatag acctacatct
     1501 ggaaagattt atgttgaaga tacagaaata tcaagcttaa atgaagaagc tcttgcagtt
     1561 tttagacgta gaaaagtagg tcttatttat caattttata atcttatacc tactcttgat
     1621 gttagaaaaa atatattatt acccatgtta cttgataaaa gaaaggtgga tgaagataga
     1681 ttctctgaga ttgtatctat attaggatta tctgatagat tgaatcatct tccaagtcaa
     1741 ctttcaggtg ggcaacaaca aagggtatct atagctagaa gtttaatcta tagaccaact
     1801 attttattag cagatgagcc aactggtaat ctcgatagaa aaaattcaga agaaattgta
     1861 gatttgttaa acttatcaaa taaacgattt aatcagacaa tacttcttat tactcatgat
     1921 gagaaaatag cactagaggc aaatcgtatt gtaactatgg aagatggagt gattgtctct
     1981 gaaaaggtgg tgaagaaatg aatattcttc aagaatatac cttagacttt gtacgacgca
     2041 ataaacgtag tagtatagct ataatgttag ccatattact taccacaacc atgatgtctt
     2101 ccttgtgtgg attgttgtat accatgtgga cagattttat aagactttca attgagaaag
     2161 agggtaactg gcatggtgaa ttgtttgacg atacttatgg aaaagactta tcgttaattg
     2221 aaaatttttc ttcagttgat gatgtaatgg taaaaggaca atggtatgta ggcaaaatta
     2281 ataatgacaa aagagattat atagtttata gaaatgctac atcagagtat tggaatcata
     2341 tgccagaaaa agattctata atagaggggc gtcgaccaag taatactaat gaaattgcac
     2401 tatcaaaaca atattttgag aataatccaa gtgtaaaaat tggtgataaa attactctcc
     2461 caataggaga tagaatccta aatggaaagc ctcttgaacc tgtagcccca aaatcgaaag
     2521 gggaaagttt taaaaaaatt tcaaagacta cattgacaat agtaggaaag gtagatgttg
     2581 ctacttcatc agttgtacct gcatatacag ctcttggata tcttgataaa acaagtataa
     2641 aacctaatga cagtataact gtgtacttgc gatttaaaaa tataagagac acatataagg
     2701 aacttccaaa acttgctaaa tcattgggat ggaagactaa tgaatatgga aaatacaatc
     2761 ttaaatacaa tagtaattat cttttaaaaa tgttagtctt ttctccagaa cagaaagcat
     2821 ctatgagcaa tatatcaaaa ttttcaactc ctattatgta tcttacaata gcaattttta
     2881 cagtagccgt gtttgtaatg gtaatttaca acgcattttc actatctgca aatgctagac
     2941 ttacacaatt aggaatatta tctagtgttg gagcatcacc aaaacagatt aaaaggtctg
     3001 tagtgtttga aggtttttta ttgactatta taccattacc aataggttta tttttagggt
     3061 ggttattgtg taataagctt atagtctata ttaattcagt taattatcat actgatgctc
     3121 cagaagtagt attcacttat ggtattcctg cttttctgcc agcagtttta cttacaatag
     3181 taacagtctg gatttctaca ctcataccag cacgaaaagt atcaaaaata tctcctatag
     3241 aagcaatacg acaaggagat agtgttaaaa ttaaaaagtc acataaatat tcattaggaa
     3301 agatatttgg aatagaagga gaacttgcat caaatgcact acgtgctcgt aaaaaatctt
     3361 atcgtactgc aacaatatct ttaacattat cttttttatt actgacaggt tttttgcata
     3421 taattgcaaa tcaagaagga gcagaagctg tttatggaag tgataattat aaagaccaaa
     3481 gagatataga agtttatttg gaaaatagtg aacctgtaga gtctgatttc attgaaaaat
     3541 tagataatac agaaggtgta aagaactcac tatattatca aaagatgtct gctaccactt
     3601 ggctgactga aaaagatgct tcaaaagaac tttcgaaaaa tggaggattt aaaagtatag
     3661 tttcatctaa aaagtactct cctatagagc gtgatggcaa gtttcgtata atttcaaata
     3721 taattgggct tgatgatgag agtttcgcag aatattgcag acaaatctct gtagacccaa
     3781 aattatttta tgatacaaaa aatccaactt caattgtata taataaggag gaagatgtta
     3841 atcgtagcac acgtagaaat aaagtatata tagattattt aaatctttca ataggagata
     3901 acattaaatt aaatgaaaaa atatatgatg aagataaagg ggattatact tacaacatga
     3961 aagtgggagc tattacaagc acacttccta aaataataga tactagttca agatatacat
     4021 taatgcaaat agtaccaata tctatggtgc aagaaatatc taaaaattat gctgaagtag
     4081 gtaggtcacg ttcaaataga ctgataggat tgtttaaagt agataatcgt gaggatattc
     4141 ctagagtaag aggaaaatta gagtctattt gtaataaaga ttatggttca ggagactata
     4201 gtattgagga tgttgtagaa aatgaaaaac aaaatggtag tggaagaaag actatgaatt
     4261 tgatagtagg ttttattaca ggtttattgg ctatgattgg attatcaaat gtacttgcta
     4321 cagtgtcagg aaatattaga agtagacgac aagagtttgc tatgttgcgt tctgttggat
     4381 tatctccaga tggcataaaa aagatgcttg ttttggaagg tttattctta ggaattacac
     4441 cattactact tagtattcct gtgcaaatag gaatagtata tacttttcta cgtataaatg
     4501 aaatatattt tatagagtat ttaccatttg cacctatatc tacaataata ggttttacta
     4561 tattgatact ttttattgta atagcatcat atatgacagg atacaatcag ttgaagaatg
     4621 aaaatatagt agaatctata aaaagtgaaa caatataaat aaaagttatc tgaacttatt
     4681 tatggtttca attcaagaat acttacgagt atagagatag ttaaattgtt tatttaataa
     4741 aatcttagat ataaatagcg gattactaaa ataataaata acataggata tagatatgac
     4801 tggattatgg gtattttacc tataatccag tattttatgt gtaaaactta attatgaatc
     4861 ttgcaccacc attatggcaa ttttctgcat gtagagtacc attttgacgt tctataatag
     4921 actttgatag agcaagccct ataccaatac tgtccttact agcgttttta ccacgataaa
     4981 atctattaaa aatatatggg agctcttcct gagcaaaccc cttaccatta tcttctatga
     5041 taatctcaac atatagaggg ttttgactat aataagcaat gatatgtcca ccagcttcta
     5101 tatgctcact acaattttta agaatattta aaagagcttc taaagtccaa ttaatatcaa
     5161 tttcaaattc tattgaagga gaacctttga tagtaaatgt ctgatgtttt tctcttaaag
     5221 aaccttctat aggctcaatt gcataagtaa gaagagaatg tacatctata agtttgcttt
     5281 caaatattaa agtactcgca tccaattttg ataaggtaag taaagatgaa actagtctct
     5341 caagtcttga tatttgcttt gtaagcctgt taatataatc tatttcttca tctggacaat
     5401 tttctgaaag taattgtgcc ataagtgaca tagaagtaat tggagtctta agctgatgag
     5461 caatatctgc aaggttatct gaaagagttt gtctttcttt tagagcaaat tcttttgaac
     5521 aacgaagttc cccaactgtt ttatatatct catcttcaag ataagaaaat tcatcttcat
     5581 atcttaaaag tacagactct tttcctaaat ttacttcttc taagtatcga gtaagcatgt
     5641 ttattctact caatttttgc ttatttgttc tatacttgat tatatataca actatagcca
     5701 ttgttgaaat tataccaaac gataccacta gaaaataaaa aaagttttta ctccaaaatg
     5761 tacctttaga atatccatgt tttgataaaa tttcatttcc tgttttaaaa tcctcatcac
     5821 tagaattttt cagtgatttt agtatacttt ctgtagattt aggattgtac tcatatgatg
     5881 catcaagaac cttagacagc acattgtatt cattaaaaga agtgatagtt gttagaatta
     5941 cactcaataa aaaagttaca attaagcaga atattataaa aacaactgta ctgttgatat
     6001 ttcttggagt tttcactagc tgtcctccat tctatatcct atacctctta atgtttttat
     6061 acaaggtgga ttttttaatt tttctctaag acgctttatt gtaacagtaa gagtgttgtc
     6121 atttacgaaa ttaccatcta catcccagag catttctagt aattttgtac gagtcatagc
     6181 tctattttta ttttccataa gacttagaag aagcttatat tctcctgctg tcagaggtac
     6241 ttcaacacta tctaaatatg ccttgacttc aactttattc aaacttatat ttccacaagt
     6301 aatagtatta ttagtattag aatgagctct tctaagaact gcattaattc tagattgaag
     6361 tactgaaagc aaaaatggtt taaccacata gtcatctgca cccatatcta atccacgtat
     6421 aatgtctttt tcatcatcac ggactgttaa aaatatcact ggagtatctt ctatactttt
     6481 aacgtattgg caaaattcgt atccaatacc atcaggtaga tttaagtcaa gcagaattaa
     6541 atcagtagtt ttatcaaaat tttttttagc ttcagcaatc gtattataac aacatacaat
     6601 gtgattttta ttttccaggg aagtctttat cccaaacgta attgtatcat catcttctaa
     6661 gagaagtatt ttagccattt acttttagtc ctttcattaa tttaaattat tagttttatg
     6721 cttaatattt tttatattat atatagtaaa gattaccatt taagtatata aattttaatt
     6781 tatatactta ggtctatgct ttaatcgagt ctattaaaat ggcaagggtt gtttcaataa
     6841 atttttcctc atctatatta aaattgttca ggtatgattt tcttgttaca gacaataaat
     6901 taaaaaatcc tgtaagtgta aatattgatg aatacataag agtagatatt tcaatatcat
     6961 acctaatact tccatcattt tttccaattt caaacatttc ttgtatttca tcaaacataa
     7021 atttattaaa agaaaaaaac ttttctttaa acagcatctc agtatccgat tttttagagt
     7081 ttacaatacc aatcattccc ataaggcata atagagaagg ttctacagag taaaatttat
     7141 gaaaagcaaa gtatgaaagt tttatttttt caaaccctgt tttacctttt gcactttcta
     7201 ttttaacatt atcccaaagt cttttatatc cccttaagac aacagcaaag aataaatctt
     7261 ctttacatga aaagtacttg tatatggtgc gtttagtata ttcgacttcc tttgccaact
     7321 cattcatagt tgtattatca aatccattaa gagaaaataa tttttctgct ttatcaataa
     7381 tttcattttc tctttgtatt ttttctttat ctctttttga taattctgac atataaatta
     7441 ttcctttcta ttgataataa agtatactgg tggtatactt gtgtaaatat aatatatttg
     7501 tatactatta gtatacttta atgaaaataa aattaatatc aagtattata gctatttata
     7561 acttcattaa ttaattatat aggcaaaata ccaaataggg ggaatttatt tatgaaaata
     7621 aaagaaaatg tattaaggtt agatagtact cgtggctcaa attgttttat tgtgtttgat
     7681 aaagagatta ctcttataga cacaggtctt ccttttatgg ggaaaaatat aattaaagaa
     7741 ttaaaaacac ttggtatcaa acttacagat attaagcata ttttattaac tcatcatgat
     7801 gttgaccata tgtctaatat taaattatta aaagagaaaa caggagcaaa ggtatgggct
     7861 catacagaag atataccttt tattatagga gagaaagata gacctgggtt taagaagttt
     7921 attggaaaga caatttcaag aaggctcatt aaggatgttg aaccttatgg agagaatatg
     7981 cagattggga atataaaaat tatccatact cctggtcata ctccagggca tgtctgcatg
     8041 atttttgaag atgtattatt tgcaggtgat ttagtcaaaa acaagaatgg aaatatagtt
     8101 tcttacccta atccctggaa ttgggattct aaaaagatga tgaaatctat aagagaattg
     8161 gataatttag aatatgaatg gatatgtatg tctcatggaa ctccttgtat taaataataa
     8221 aaataataag ttgtaatata tcctatgtta tctattgact atatccaaga ttggtgataa
     8281 tataatatcc aagatagagt ataggtggtg gatttttatg atacgcactt taattttata
     8341 ttatctaaat attaagccaa cacatgggta tgagattcaa aagtttttac aagtatctgg
     8401 tgcagataga tggactaaaa tacaatctgg gtcaatatat tatgcattgg caaaattaga
     8461 aaaagatgga ggcgttagag ttttaaaaga agaaaaaaca ggagctagga ttcgtaaaat
     8521 ctatgagatt actcaaagtg gtaaattaga attaagggaa gaaatacaaa aagagcttca
     8581 gatgccaata gttccaaccg gttcaaataa attcctatta cataatattt tggatgtatt
     8641 acctaaggat actttgcaaa aaaatcttga aaaacatata aaatatctta tagagcagaa
     8701 gaagtattgg gaaaattgga aagagattaa aaaaattgat aaaaagagtc ttgcaacaga
     8761 aaaaattgca tttgatatga ctatagataa tctgaattat caaattttat ggcatgaaga
     8821 aatattaaat gatattgata agtatatatc cgttggttgc gagacacaga atataattaa
     8881 atctatagat ttttcaaata tagaagaaga ttttttattt acatctgata cgacaaatga
     8941 gttattagaa gttcagagac ttagagatga aataattaat aacccagata aagctataga
     9001 aaacatagat aagataatac taaaattaca aaataagtaa taaattttat tacaataaca
     9061 cgggtaaaat actcgtgttt taaaaaaatt atatatacaa tattggatat ataatgttgg
     9121 ttaaatattg gggggttata aatgagtaat atattgttag agattaaaaa tttatctaag
     9181 aagtataaca ataaggaagt tgtgtcaata gaaagtttta agttattgga aggcgatatt
     9241 ttaggtttca ttggagcaaa tggagcaggg aaaagtacat ctatcaatat gcttactacg
     9301 attgtgtctc cagatagtgg aagcatatac tataaaggaa aagatattaa gaatcaacaa
     9361 aatattttta aaagttcact tggagttgta ccacaagaat tagcaatata tgaagattta
     9421 agtgcatatg ataatgttaa atttttttgc tcattatatg gatttaaagg gaatgaactt
     9481 aaagagagaa ctaaagatgc tttggaattt gttggtcttt gggaaagcca taaggagttt
     9541 ccatcaaaat tttctggtgg aatgaaacgt agactaaaca tagcttgtgc aattgcacat
     9601 agtccaaaga ttctcatcat ggatgagcca acagtaggta tcgaccctca atcaagaaat
     9661 aaaattttgg aggcaatcaa atgcattaat actaatggta cgacagttat atatactagc
     9721 cattatatgg aagaaattga aaccttatgt aatagaattg ctttaataga taaaggaata
     9781 at
//
LOCUS       DAFNNB010000380         9589 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0150, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000380 DAFNNB010000000
VERSION     DAFNNB010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 9589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9589
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0150"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(<1..388)
                     /locus_tag="L3M60_003711"
     CDS             complement(<1..388)
                     /locus_tag="L3M60_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002569775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HBN6228294.1"
                     /translation="MAKHAILRFEKHKGNPARPLEAHHERQKEQYASNPDIDTSRSKY
                     NFHIVKPEGRYYHFIQSRIEQAGCRTRKDSTRFVDTLITASPEFFKGKSPKEIQAFFQ
                     RASDFLIGRVGRENIVSAVVHMDEKTP"
     gene            complement(571..966)
                     /locus_tag="L3M60_003712"
     CDS             complement(571..966)
                     /locus_tag="L3M60_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HBN6228295.1"
                     /translation="MTNTIYIHQPEKAFSFTRLPNFLFEAPTFKPLSNEAKVLYAFIL
                     RRTELSRKNGWADDCGRIFLYYPICEVVTLLHCGRQKAVNTLRELQYAGLVEIQKQGC
                     GKPNRIFPKSYEAVPNTDFKKSGSGTPED"
     gene            complement(1078..1491)
                     /locus_tag="L3M60_003713"
     CDS             complement(1078..1491)
                     /locus_tag="L3M60_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich VLP domain-containing protein"
                     /protein_id="HBN6228296.1"
                     /translation="MRDNPYKDLPPLERRPDGSLYRMTPAQRKQAASLIRRECCCCED
                     GNCIVLDDGDTCTCPQTISFSVCCKWFRWAVLPLDGTLEAEIVRDKDLKRCAVCGGVF
                     VPKSNRAKYCPGCAARVHRRQKTESERKRRSAVDS"
     gene            1831..2628
                     /locus_tag="L3M60_003714"
     CDS             1831..2628
                     /locus_tag="L3M60_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008666013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBN6228297.1"
                     /translation="MALPGTPGQRISDLCNGNHITQKELAEKIGVSASQLSRIVSGET
                     RTVSSDILIGVAKEFKVSTDYILGLSTVSVRKSYDISELGLSEGAVRGLVTGAVDVQI
                     LNRLLEHRNFPKLIDLIRIYFQDTAAKGITARNQLIEIATASLSDLMKEHPEHRAEAK
                     QDLQLLNAQKMGEHEAEIEKIKNVFLAILRDIKKDIDNGEQPGEAVTAAMFQAMRDAL
                     AEQKQNPLSIDDVAAMVAGQIGQLTPMDEETADLFKQLAKKMMKGIE"
     gene            2653..3423
                     /locus_tag="L3M60_003715"
     CDS             2653..3423
                     /locus_tag="L3M60_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HBN6228298.1"
                     /translation="MPLVKHDYPVLEYDTASKAVFQPGNGKKCFPAKAVFAFLGDEVE
                     NYAHTHDGIQIDEFESATRRYPIYECLYNQEKICLCPAPVGSAAAVQVLEYLIAGGVT
                     KIISVGSCGVLEDIPENRFLIPVSALRDEGTSYHYLPPSREVEISKAGINAIESALSQ
                     KNIPYWEVKTWTTDGFYRETVEMVQYRKEEGCQVVEMECSALAACAKFRKVTWAMLLF
                     SADTLADPHKYQEREWGKTSISIALELALDAVLSVVEE"
     gene            3448..4224
                     /locus_tag="L3M60_003716"
     CDS             3448..4224
                     /locus_tag="L3M60_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(9) family aminoglycoside
                     nucleotidyltransferase"
                     /protein_id="HBN6228299.1"
                     /translation="MNINEFPQQVNQVISIAETILQGQILGIYLYGSATMNGLRPDSD
                     IDILIITKQELSNSIRADLTKQLLKISGSVGCIEKRPLEVTIINQSDIVPLQFPPKCQ
                     YMYGEWLRGEMEAGEYPQACNDPDIMILLWQARKNSITLKGAESKELIPAIPFHEIKK
                     AIRFSLPGLISSFKGDERNVLLTLSRMWFTLVTEEITTKDVAAKWVILKLPERFPPLL
                     TTAKEAYLGNLSDEWETVEKEAMALVEYMKKQIEELLRTE"
     gene            4424..4969
                     /locus_tag="L3M60_003717"
     CDS             4424..4969
                     /locus_tag="L3M60_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002578721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBN6228300.1"
                     /translation="MNQGRIIVITGSPGTGKTTTASIVAKESNMDKSVHMHTDDFFHY
                     LSKGAIPPHLPESNEQNLVVIEAFLEAAKRYARGGYDVIVDGIVGPWFLEPWRALVRE
                     DYEVHYIVLRASKEETMKRAVERSKLDRKTNVELVETMWEQFCNLGIYESNVIETTTY
                     SIQEAVFAVKEKISSGAALLS"
     gene            complement(5708..5827)
                     /locus_tag="L3M60_003718"
                     /pseudo
     CDS             complement(5708..5827)
                     /locus_tag="L3M60_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011861830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(6265..8214)
                     /locus_tag="L3M60_003719"
     CDS             complement(6265..8214)
                     /locus_tag="L3M60_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBN6228301.1"
                     /translation="MKRKYPNLCKPIKIGNVTFKNRMFSAPMGGTDITADCTIGSKST
                     AFYELRAKGGAGAVTISECMVHPETDGSHAYHLDLKIVDSLASFTYTADAIRRHGAIP
                     SVELSHSGQYSGTYLVDKDKKRGLSQWGVSPSVRPDGLEIKELTEEIIAEIVESYGNV
                     AALAKRAGFEMIMVHGGHGWLINQFLSPYFNKRNDKYGGSLENRVRFAKEVLDSVRKA
                     VGPGFPIEFRMSGSELFEGGYDLDYGVEIAKLLESRVDLLHVSAGTYQRGFAVTHPSM
                     FLEHGCNVYLAEEIKKHVNIPVATIGALNDPEMMEEIIASGKADVIYMARALLADHEL
                     PRKVMSNQDEKIVKCLRCFTCMAERATTSTRRCTVNPLIGRELDGTEVIPVLKPKKVL
                     IAGGGPGGLQTAITAAKRGHKVILCEKTNELGGILKGEQALPFKYEMYELGNTFGKIA
                     EDLGVEVRLNTRVTKEYVENENVDALIIAVGSEPLVPSIEGLDGENVVIVNDYYLEKE
                     KVTDEVVVLGGGLAGCEAAIHLAQEGKTVHLVEMRAELAPDANIRNRPILLQEIEKQG
                     IYVYTEHKGLSVTTEGVVCMNKSGNKINIPGTSVICALGQKPRREVVDALLDCAPYVA
                     QIGDCVRASTITNAVYQGHHVALDI"
     gene            complement(8186..9484)
                     /locus_tag="L3M60_003720"
     CDS             complement(8186..9484)
                     /locus_tag="L3M60_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009891479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HBN6228302.1"
                     /translation="MNLTQKRWIILIASCFINLCIGSIYAWSVFASPMAEHLNSIAGT
                     TFTAASLAIVFTVTNSVGPITMITGGMINDKFGPKKVVFVGGLVFGIGMILAGFSKGL
                     PMLILSYGIITGLGVGMVYGCTISNSIKFFPDKSGLVGGITTAAYGFSSVIMPPVANW
                     IINKYGVTSAFKSIGLVFLIIVCVSSFFIKKCPADFTPEGWIPPEKKGKVRVIINKNW
                     KEMLSTPIFYLMIIILTCGAFYGLMCVSLASSLATNMIGMTSSDAAIVVSILALFNTL
                     GRIVAGYISDKIGRINTLAGALVISIFGLLCLLFSREGDVATFYIGIIIIGICFGSFM
                     GIFPGFTVDKFGPKNNSVNYGIMFIGFAFAGYVGPAVMKNVYATDGNYQRAFIIAIVI
                     ALIGFILTFLYKYINKIQNLKIENLMKGDIKDEKKISKSM"
BASE COUNT         2820 a         2019 c         1856 g         2894 t
ORIGIN      
        1 ggggcgtttt ctcgtccatg tgtaccaccg ccgacacgat attttcccgt cctacccggc
       61 caatgaggaa gtccgacgcc ctttggaaga acgcctgtat ctcctttggg gatttcccct
      121 taaaaaactc cgggctggcg gttatcagcg tatcgacaaa ccgtgtgctg tccttgcggg
      181 ttcggcaccc ggcctgctca atccggctct gaatgaagtg gtaatagcgt ccctccggct
      241 tgacgatatg gaagttgtat ttgctccggc ttgtgtcaat gtcgggattg ctggcatact
      301 gttctttctg tctttcgtga tgggcttcca gcggccttgc cgggttgccc ttgtgcttct
      361 caaaccgtaa aattgcgtgt tttgccattc tgctcctttc cccattcctt tcctatccgg
      421 ggttttccac agggaaaatc ccgcagaaaa tagctcggat aggattggaa tggatagaat
      481 ataaatcaat ctttttatct ttgtatttct atataccgtc cggttttcgt acgtctcagg
      541 agtgatttcc gtacttcatg ggtacggttt tcagtcctcc ggcgtaccag aaccggattt
      601 cttgaagtcg gtgtttggaa ccgcttcata ggattttggg aaaatgcggt tgggttttcc
      661 acagccctgc ttctggatct ccaccagtcc ggcgtattgc agttcccgca gggtattcac
      721 cgctttctgc cgcccacaat ggagcagtgt gaccacttca cagatagggt aatacaggaa
      781 aatccgtccg cagtcatccg cccacccatt cttgcgggat aactctgtcc ggcgcaggat
      841 aaaggcgtac agaacctttg cctcgttaga caggggcttg aatgtggggg cttcaaagag
      901 gaaattcggg agccgggtga agctgaacgc cttttccggc tgatggatat agatggtatt
      961 tgtcatagtt cgttttgtgg acggttagaa gcccgttttc cggggcgggc gatactttat
     1021 accacctgcc ccggtttggg gcttgcaaag cctgataaat caaggctttt ttcgctccta
     1081 actgtccaca gcagacctcc ttttccgttc actttctgtt ttctgccgcc tgtgaactct
     1141 ggcggcacag ccggggcagt attttgcccg gttggatttg gggacgaaca cgccaccgca
     1201 gaccgcacag cgtttcaagt ccttatcccg gacaatttcc gcttccagcg tcccgtccag
     1261 cggcaagacc gcccagcgga accacttaca gcagaccgag aaagaaatcg tctgcgggca
     1321 ggtgcaggtg tccccatcat caaggacaat gcagttgccg tcctcacagc aacaacactc
     1381 ccggcgtatc aggctggccg cctgttttct ctgcgccggt gtcatgcggt aaagggaacc
     1441 gtccggcctg cgttccagtg gcggcaagtc tttatagggg ttatctctca tgcgttccct
     1501 ccattttgct ttccgttgcc gttctccccg aaaaggtttc ctgccccatt cctgcggcgt
     1561 gttccggcgt tggcacgctc cccgcagctt cgggacattt tgtctcgaag tccggttcct
     1621 tgcggtgctt ccccagccgg ggtattcctt ttcggacggt cattctgttt tcaaggtgct
     1681 gtccatcgat gaactaccct aagtctacac gaaaaaaatg caatccccgg aattttgcga
     1741 gaattgcatt ttgatgaagt ttacactttg gaaattgcat tttttgcaat cattctgtat
     1801 aatggaagta tgcggaggag gtgcgtatct atggctttac ccggaacacc cggacagcgg
     1861 atttccgatt tatgcaacgg gaaccacatc acacaaaagg aacttgcgga gaagataggg
     1921 gtttccgctt cccagttgag ccgcattgtc agcggcgaaa cgagaacggt cagcagtgat
     1981 attctcatag gcgtggcaaa ggaatttaag gtatcgacag actacatact gggcttatcc
     2041 accgtgagcg tccgtaaaag ctacgatatt tctgaattag gcttgtccga gggagccgtg
     2101 agggggctcg tgacgggtgc tgttgatgtg caaatcctca accgcctgtt ggaacacagg
     2161 aattttccta agctgataga tttgatacgg atttattttc aggacacagc ggcaaaaggc
     2221 ataacagcaa gaaaccagct tatcgagata gcaacggctt ccctgtccga cctgatgaaa
     2281 gaacacccgg aacaccgggc ggaagcaaag caggatttac agcttttgaa cgcccagaag
     2341 atgggggaac atgaggcgga gattgagaaa atcaaaaatg tgttccttgc tatcctgcgg
     2401 gatattaaga aagacattga caacggggaa cagccgggag aagctgtgac cgccgcgatg
     2461 ttccaagcca tgcgggacgc attggcggaa cagaagcaaa acccgctttc cattgacgat
     2521 gtagcggcga tggttgccgg acagatcgga cagcttacgc cgatggatga agaaactgcc
     2581 gacctgttca agcagttggc aaaaaagatg atgaaaggaa tagaataata gcctgttatt
     2641 cgagataata atatgccatt agtaaagcat gactaccctg ttttagagta cgataccgca
     2701 tcaaaagctg tttttcagcc gggaaatggg aaaaaatgtt ttcctgcaaa agcagtgttt
     2761 gcttttttag gagacgaggt tgaaaattat gcacataccc atgatggaat acagatagat
     2821 gaatttgaaa gtgcaacaag acgatatcct atttatgaat gtctttataa tcaggagaaa
     2881 atatgtctat gtccggctcc tgtgggaagt gctgctgccg tccaagtttt agaatattta
     2941 attgcagggg gtgtaacaaa gattatttcc gtcggctcct gcggcgtatt ggaagacatc
     3001 ccggaaaata gatttttgat acctgtttct gcattgagag atgagggaac atcttaccat
     3061 tatcttcctc cctcccgaga agtagagatt tcaaaggctg gtataaatgc gattgaatct
     3121 gctttaagcc aaaagaatat accatattgg gaagttaaaa catggacaac agacggtttt
     3181 tatcgagaaa cagtagaaat ggttcagtat cggaaagaag agggatgtca agtagtagaa
     3241 atggaatgtt ccgcattggc ggcgtgtgca aaatttagaa aagttacatg ggctatgctg
     3301 cttttttcag ccgatactct tgcagaccct cataaatacc aagaaagaga atggggaaaa
     3361 acaagtatat ccatagcctt agaattagcc ttagacgctg ttttatcagt tgttgaggag
     3421 taaaaataaa tacatatagg agttacaatg aatataaatg aatttccaca acaagtaaat
     3481 caagttattt caatagcaga aaccatatta caaggtcaaa tattggggat atatttatat
     3541 ggttcagcaa caatgaatgg gctgcgtcca gatagtgata tagatatact gataattact
     3601 aaacaagaat tgagtaattc aatcagagca gatctaacaa agcaattatt gaaaatttct
     3661 ggctccgtag gctgtattga aaaaaggcct ttagaggtaa ctattatcaa tcaatctgat
     3721 attgttccgt tgcaatttcc gccaaaatgt cagtatatgt atggtgaatg gctaagggga
     3781 gagatggaag caggagaata tccgcaagcc tgcaatgacc cagatataat gattttatta
     3841 tggcaagcaa gaaaaaatag cataactttg aagggggcgg aaagcaaaga gcttattccc
     3901 gctatcccat ttcacgaaat taaaaaagca attcggtttt ctttacctgg tttgatttcc
     3961 agctttaagg gtgatgaaag aaatgtgtta ttaaccttat cacgaatgtg gtttacttta
     4021 gtaacagaag aaatcacgac aaaagatgtt gccgcaaaat gggtaatttt aaaattgccg
     4081 gagagattcc cccccctgct aacaacggca aaggaagctt atttgggaaa tttgtctgat
     4141 gaatgggaga cagtagaaaa ggaagcgatg gcacttgtag aatatatgaa aaaacaaatt
     4201 gaggaattac ttagaacaga gtagcaaagt tagcggggcc aatcaacggt caagatgaac
     4261 ggcgcataaa tgcgccgccg ttgacagtcc cgcccgtctt tgctgatggg caatcaaggc
     4321 gggaaagccc taaaatggct tcccgcccat ttcaatcttg agagaagaaa cgctccaaaa
     4381 ccagaaagag agcggtaaat ctgaattgcg agggagaatg tagatgaatc aaggaagaat
     4441 tattgtgatt acaggttcgc cgggaacagg aaagacaaca actgcgtcga ttgtcgcaaa
     4501 agaatcgaac atggataaat ctgtgcatat gcacacagac gacttttttc attatttaag
     4561 caaaggagca ataccgccgc atttaccaga gtccaacgaa caaaatttag ttgtcattga
     4621 agccttttta gaagctgcaa agcgctatgc ccgcggcgga tatgatgtaa ttgtagatgg
     4681 aattgtcggg ccgtggtttt tagaaccatg gagagccctt gttcgagaag attatgaagt
     4741 acactatatt gttttaagag ccagtaaaga agaaactatg aaacgagctg tggaacgctc
     4801 aaaattagac agaaaaacaa atgttgaatt agtagaaaca atgtgggagc aattttgtaa
     4861 tttggggata tatgaatcga atgttataga aacgacaacc tattctatcc aagaggctgt
     4921 ttttgcagta aaagaaaaaa tttcaagtgg agccgcattg ctatcttaaa cgatttctaa
     4981 aaatcgaaat ttcatttatc agagaaatag caaaggcagc caagccagtc aacggtcaaa
     5041 atgaacggcg catttcatgc gccgccgttg acagtcccgc ccgtctttgc agatgggcaa
     5101 tcaaggaggg aaagccctaa aatggcttcc cgcccatttc attatgagaa aagaaacgct
     5161 ccaaaatcag aacgagagcg gccaagcact ttgagggatt ggccgccttg tccacccatt
     5221 cagcgggacg gcgggtggcg gtcaaggccg ggtgtaaacc cgttcatttc agccttgacg
     5281 gttgcccgct gtcctgttac ttttccggga gtgtagccac aacttcggga cactttgtcc
     5341 acaagtcgga gcgtaggacg agggacgggg gctttcatat acgcccagtc tgctgttgaa
     5401 accagacctg tgcttacggc tctacgccac gcaagcacag ccctgttttc ctgccgcttc
     5461 aaatgccctt gccctccccg gcggcaacgg cattttcacg gcaacgccga cagagcgtat
     5521 aacacactac actttgcttc gcaaagtcgt gtgccaaatg ggggcgttgc cccctttgga
     5581 aacccccaca agaaaaggcg gtacgctacc cctccacggg gcgcataccg ccttttcttg
     5641 ttatccagcc ctccggctgt atcggttgag caaaagtcag cgtgggaatg gtgtggtatc
     5701 tttatctaag tgatacccca ctttcccatt ttgctattga ttgtctagaa acattaagtt
     5761 tttcagctaa ttcttcttgt gtatactggt ggatttttct tagacttttt aaattctcat
     5821 taatcatatt tttcatacct cctatgttta taatattgga tttattattt aaattctatt
     5881 aactaatctt aacattcata tatatattta tatacttttt attagataaa tttattgttt
     5941 aggtaacata cttccttaag ttataataaa taaaagcaag cacaaatcta tcaacttttg
     6001 ctaaagtatt taagttagag tcatttacat tgttaaatac ttctacacct taaaaagttt
     6061 tactcatcaa tcaaatgaaa acttgcaata agaattaaaa ttataataac aaaggtgctg
     6121 tctcaaaata aagataatct tttttcatta ttgaaatgat aaaaatatat ttgatttcac
     6181 gcaaaaaagt gtctcatgaa ctaaaaagtt cattttgaga cactcctttt ttataataaa
     6241 acaatactat ctacttcttt ttaactatat atctagcgca acatgatgcc cttggtatac
     6301 tgcatttgtt attgtagatg cacgaacaca atctccaatt tgagcaacat atggagcaca
     6361 atcaagtaaa gcgtctacca cttctcttct tggtttttgt ccaagtgcac atattactga
     6421 agttcctgga atatttattt tattcccaga tttattcata catactacac cttctgttgt
     6481 tacacttaaa cctttatgct ctgtatatac atatatacct tgtttttcta tttcttgtag
     6541 taatattgga cgattcctta tatttgcatc cggggcaagt tctgctctca tctctactaa
     6601 gtgaacagtc ttcccttctt gagccaagtg aattgcagcc tcacatcctg ctaatcctcc
     6661 acctaaaact actacttcat cagttacttt ttctttttct aagtaataat cattaactat
     6721 tactacattt tctccatcaa gaccttctat tgatggaaca agaggttcag accctactgc
     6781 tataattaat gcatcaacat tttcattttc aacatattct tttgtaactc ttgtatttaa
     6841 acgtacttca acacctaaat cttctgctat ttttccaaaa gtatttccta attcgtacat
     6901 ttcatattta aatggaagtg cttgttctcc ttttaaaatt ccacctaatt cattagtctt
     6961 ttcacaaagt attactttat gcccacgctt tgcagctgta atagctgttt gtaaccctcc
     7021 tggtccacca cctgctatta atactttttt aggttttaat actggaataa cttcagttcc
     7081 atctaattca cgtccaataa gtggattaac agtacaacgt cttgtagaag tggtagcacg
     7141 ttctgccata caagtgaaac atcttagaca ttttactatt ttttcatctt gattactcat
     7201 aacttttctt ggtaattcgt ggtcagctag taatgcacga gccatataaa taacatctgc
     7261 ttttccacta gcaattatct cttccatcat ttctgggtca tttaatgctc ctatagtagc
     7321 aactggaatg tttacatgtt ttttaatttc ttctgcaaga tatacattac atccatgctc
     7381 taagaacata gaaggatgtg ttactgcaaa tcctctttga tatgtacctg ctgatacatg
     7441 aagtaaatct actcttgatt ccaataattt agcaatttct actccataat ctaaatcgta
     7501 tcctccctca aataattcag aaccactcat tctaaactct attgggaatc ctggacctac
     7561 tgctttacga acactatcta aaacttcttt agcaaaacga actctatttt ctaaagaacc
     7621 accatattta tcatttcttt tgttaaagta aggtgataag aattgattga ttaaccatcc
     7681 atgtccacca tgaaccataa tcatctcaaa ccctgctctt ttagccaatg ctgcaacatt
     7741 tccatacgat tctactattt cagcaattat ttcttctgta agctctttta tctctaatcc
     7801 atcaggacga acactaggac ttactcccca ctgtgataat cctcgcttct tatctttgtc
     7861 aactaaataa gttcctgaat attgtcctga atgagataac tctacacttg gtattgctcc
     7921 atgacgacga attgcatctg ctgtgtaagt aaaacttgct aaagagtcaa ctatttttaa
     7981 atctagatga tatgcatgtg aaccatctgt ttctggatga accatacatt cgctaattgt
     8041 aactgcacct gctccacctt ttgctcttag ttcgtaaaag gctgttgatt ttgagccaat
     8101 tgtacaatct gctgttatgt ctgttcctcc cataggtgct gaaaacattc tatttttgaa
     8161 tgttacattt ccaattttaa ttggcttaca tagatttgga tattttcttt tcatctttta
     8221 tatctccctt cattaaattt tctattttta aattttgtat tttatttata tatttatata
     8281 aaaatgttaa tataaaacct attaaagcta taactatagc tataatgaaa gccctttgat
     8341 aattgccatc tgtagcatat acatttttca taactgcagg tccaacatat cctgcaaaag
     8401 caaatccaat aaacatgatt ccataattta cactattatt tttaggtcca aatttatcta
     8461 ctgtaaatcc tgggaaaatt cccataaatg aaccaaagca tatacctata attattattc
     8521 caatatagaa cgttgctaca tctccttctc ttgagaataa taaacataat aaaccaaaaa
     8581 ttgaaataac taatgcacct gctaaagtat ttattctacc aattttatct gagatatacc
     8641 ctgcaacaat acggcctaat gtattaaata gggctaaaat tgaaactact attgctgcat
     8701 ctgaacttgt cataccaatc atatttgttg ctagagaaga tgctaatgac acacacatta
     8761 atccatagaa agctccacaa gttaatatta taatcattag ataaaatata ggtgttgaaa
     8821 gcatttcttt ccaattctta ttaataataa ctcttacttt tccttttttt tctggtggta
     8881 tccatccttc tggtgtgaaa tctgctggac attttttaat aaagaaagaa gaaacacata
     8941 caattataag aaatactaaa cctattgatt taaatgcaga ggttactcca tatttattaa
     9001 ttatccaatt agcaactgga ggcattatta ctgaactaaa cccataagct gctgttgtta
     9061 taccaccaac taatccactt ttatctggaa aaaatttaat tgaattactt attgtgcatc
     9121 cataaaccat gccaacccca agacctgtta ttatcccata tgataaaatc aacataggta
     9181 atccttttga aaaacctgcc aatatcatac ctataccaaa aactaatcct cctacaaata
     9241 caaccttttt aggaccaaat ttatcattta tcattccccc tgtaatcata gtaataggtc
     9301 caactgagtt tgtaactgta aatacaattg ctaaactagc agctgtaaaa gtcgttccag
     9361 caatactatt taaatgttct gccataggtg atgcaaatac actccatgca tatattgagc
     9421 caatacataa attaatgaaa caacttgcaa ttaatataat ccatcttttt tgtgttaaat
     9481 tcattattct cctcctctat aaatttatta aatggaattt cttagttaag attatcacaa
     9541 ttttttctat atgtaaatat aaacatgtaa atatttacat gtttatatt
//
LOCUS       DAFNNB010000381         3385 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0230, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000381 DAFNNB010000000
VERSION     DAFNNB010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0230"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            <1..187
                     /locus_tag="L3M60_003721"
     CDS             <1..187
                     /locus_tag="L3M60_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003416276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HBN6228303.1"
                     /translation="LIIERNKLKNPIYIGDTQGDANSAKFAGIPFIYAKYGFGNVDVY
                     DYFIDSFKDLLEHDILK"
     gene            242..898
                     /locus_tag="L3M60_003722"
     CDS             242..898
                     /locus_tag="L3M60_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HBN6228304.1"
                     /translation="MELQDTIFKRQSVRKFKNQDVSDEDILKMIKAAGAAPSGKNIQN
                     WHFVIIKRRDLMEKIADVITKKQQEILVEMEKVSVDKANRFRKFVKNFTLFYLKAPVL
                     VLVFTKVYNPSGYYELELIDAPKETIDKLFIRNPGMQSLGAAIENFTLSAIELGYGSC
                     WLTSQNYAADEIEAVLEAETGFEKGEYFLGAMLALGVPEDNLKSPSKKPVEEICTFIK
                     "
     gene            1293..2015
                     /locus_tag="L3M60_003723"
     CDS             1293..2015
                     /locus_tag="L3M60_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012422616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBN6228305.1"
                     /translation="MNSYNILVVEDEKEIADAIEIYLLNQGYNVFKGYNGLEGLKVIE
                     NQEIHLAIIDIMMPKMDGITLTMKLRENHNFPVIMLSAKSEEVDKIMGLNIGADDYVT
                     KPFKPLELLARVNSQLRRYTKYLNMVENKEQKVDDDDGVFAIGGLELNENTKEVSVDG
                     KHIKATPIEFKILSLLMRNAGRVFSADEIYERVWNDNAVNTDTVMVHVRNIREKIEVD
                     PKNPKYLKVVWGVGYKIEKIQR"
     gene            1984..>3385
                     /locus_tag="L3M60_003724"
     CDS             1984..>3385
                     /locus_tag="L3M60_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HBN6228306.1"
                     /translation="MDTKLKKFKDKYIVTIIVFILMLSASLGMISQYSTIQAGAKGGA
                     KNPFEQESFVDNMYKGSYVLYHNMKEEQEGKMIQPSKLFISKETMNYIKNVSKEKDTY
                     DGSETYYTDDSINADFNQNFENWESFIKESGKNLKYYLVDKESNFEFFNDNKELGALD
                     TTKNYASKEDSDENKSKNTEMSDEERQKQIKDIKENYRFYIVMNFDKDGKVNVLDSYG
                     VNQKVISQMLLSKAREDLMNTTLGGNPISYKLSPIKNVTLVYAVPKVISKGDGGEYSY
                     GNIYNNDSISSYINNVENNSYYKISGMILKIILAIVVIVALAFPYRKSKELLGFDTLS
                     RIPLEILFIILAILQGIVDLYPTSIISNTLSGNYVEYLINNDISAKMASILVSLMNLT
                     YWFVIFSIIFVFIILLKHILNVGIKEYFIKNTVTGMFFVWLIKSGKVVINQIRITDLK
                     EKPNKTIAIILGINLLI"
BASE COUNT         1425 a          302 c          583 g         1075 t
ORIGIN      
        1 acttataatt gaaagaaata aattaaaaaa cccaatctat attggagata ctcaaggtga
       61 tgcaaatagt gctaagtttg caggtatccc ttttatatat gctaaatatg gatttgggaa
      121 tgtagatgta tatgattatt ttatagactc atttaaagat ttgttagaac atgatatatt
      181 aaaataggat gagtttaaaa attatattaa aataataaaa aagttatggg gggatttaaa
      241 aatggaatta caggatacta tttttaaaag acaaagtgta agaaagttta aaaatcaaga
      301 tgtgtcagat gaagatattt taaaaatgat aaaagcagca ggtgcagctc catcaggaaa
      361 gaatattcaa aactggcatt ttgtcattat aaaacgtcgt gacttaatgg aaaagatagc
      421 agatgtgata actaaaaagc aacaagaaat acttgtagaa atggaaaaag tatcagtaga
      481 taaggctaat agatttagaa aatttgttaa aaactttaca ctattttact taaaagctcc
      541 agttttagta ttagttttta caaaggtata taatccatca ggatattatg aattagagct
      601 tatagatgca cctaaggaaa ctatagataa attattcata agaaatccag gtatgcagag
      661 tttaggtgct gcgattgaaa actttacatt atcagcaatt gaacttgggt atggttcttg
      721 ttggttgaca agtcaaaact acgcagcaga tgaaatagaa gctgttttag aagcagaaac
      781 tggatttgaa aaaggagagt attttttagg tgcaatgttg gctcttggag tgccagagga
      841 taatttgaag agcccatcta aaaaaccagt agaagaaata tgtacattta taaaataaaa
      901 gacaataatt tttaagattc aaaatgagtt aaaaataaat agttaaaaat aaaaaggtag
      961 gttttattga tattaagttt atgtttcaaa tcattgattt aattaatgca ataaaaacta
     1021 ccttttttgt gcatattaat tttgaataac tccaaatctt tagaaaatct ttagaaaagt
     1081 atatgtaata tcttaagttt ttaactttaa tattaagtat ataaaatgaa aaaactaaat
     1141 ttatactaga gggaggtctc taaggtgtac attaaataaa aataactatt agaaagatat
     1201 aaatatataa aaaaatggaa aaacttctat catattgtat aatcatatat gtaaagaaaa
     1261 tttataaatt aattggtata aaggagatta aaatgaattc atataatatt ttggtggtag
     1321 aagatgaaaa ggaaatagca gatgctatag agatatacct attaaatcaa ggatataatg
     1381 tttttaaagg atacaatgga ttagaaggac ttaaggttat agaaaatcaa gaaatacatt
     1441 tagcaataat tgatataatg atgcctaaaa tggatggaat aacacttaca atgaagctta
     1501 gagaaaatca taatttccca gtaataatgc tatctgcaaa atcagaagaa gttgataaaa
     1561 taatggggct taatataggg gcagacgatt atgtaacaaa accttttaag cctttagagc
     1621 tattggctag agtaaactca caacttagaa gatacactaa gtatttaaat atggttgaaa
     1681 ataaagagca aaaagtagat gatgatgatg gcgtgtttgc aataggtgga ttagaattaa
     1741 atgaaaatac aaaagaagtt agtgttgatg ggaagcatat aaaagcaact cccatagaat
     1801 ttaaaatact aagcttactt atgagaaatg caggaagagt tttttctgct gatgaaatat
     1861 atgaaagagt ttggaatgat aatgctgtaa atacagatac agttatggta catgttagga
     1921 atataaggga gaaaatagaa gtagacccaa aaaatcctaa atatttaaag gtggtgtggg
     1981 gtgttggata caaaattgaa aaaattcaaa gataaatata tagttactat tatagttttt
     2041 atacttatgt tatcagcatc attaggtatg attagtcaat attctactat tcaagcaggg
     2101 gctaaaggtg gagcaaagaa tccatttgaa caggaaagtt ttgtagataa tatgtataaa
     2161 ggaagttatg ttttatatca taacatgaaa gaagaacaag aaggtaagat gatacaaccc
     2221 tcaaaacttt ttataagtaa agaaactatg aactatataa aaaatgtaag taaagaaaaa
     2281 gatacttatg atggctcaga gacttattat acagatgata gtattaatgc agattttaat
     2341 caaaactttg aaaattggga gtcttttatt aaagaatcag gaaaaaattt aaaatattat
     2401 ttagttgata aagaaagtaa ctttgaattt tttaacgata ataaagaatt aggagcactt
     2461 gatacaacaa agaattatgc ttcaaaagaa gattctgatg aaaataaatc aaaaaataca
     2521 gaaatgagtg atgaggaaag acaaaagcag attaaagata taaaggaaaa ctatagattc
     2581 tacattgtaa tgaattttga taaagatgga aaagtaaatg ttttggattc ttatggagta
     2641 aatcaaaaag ttattagtca aatgttattg tctaaagcaa gagaagattt aatgaataca
     2701 actttaggtg gaaatccaat ttcatataag ctatctccaa taaaaaacgt gacattagta
     2761 tatgcagtac ctaaggtaat aagtaaaggg gatggtggag agtatagtta tggaaatatt
     2821 tataataatg atagtataag ttcatatata aataatgtag aaaataattc ttactataag
     2881 atttctggaa tgattttaaa aattatatta gctatagttg tgattgtagc tttagcattc
     2941 ccatatagaa aatctaaaga attactagga tttgatacat tatcaagaat accattagag
     3001 atattattta ttattcttgc tattttacaa ggaattgtag atttatatcc tactagtata
     3061 ataagtaata ctttgagtgg aaattatgta gaatatctga taaacaatga cataagtgct
     3121 aaaatggcta gtatattggt aagtttaatg aatctaactt attggtttgt aatattctca
     3181 ataatatttg tatttataat acttttaaaa catattttaa atgttggaat aaaagagtat
     3241 tttataaaga atactgtcac aggaatgttt tttgtatggt tgataaaatc aggaaaagta
     3301 gttattaatc aaattagaat aacagattta aaagaaaagc ctaataaaac tatagctata
     3361 attttgggaa taaacttatt aatta
//
LOCUS       DAFNNB010000382         2698 bp    DNA     linear   BCT 21-JAN-2022
DEFINITION  TPA_asm: Clostridioides difficile isolate
            8b495a7c-6334-4cb4-a619-a14dbf286c5a
            SAMN17377984-rid13774423.guided.0254, whole genome shotgun
            sequence.
ACCESSION   DAFNNB010000382 DAFNNB010000000
VERSION     DAFNNB010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN17377984
            Sequence Read Archive: SRR13480832
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Clostridioides difficile (Clostridium difficile)
  ORGANISM  Clostridioides difficile
            Bacteria; Bacillota; Clostridia; Peptostreptococcales;
            Peptostreptococcaceae; Clostridioides.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 21-JAN-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001228449.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001228449.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/22/2022 00:15:54
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,726
            CDSs (total)                      :: 3,675
            Genes (coding)                    :: 3,597
            CDSs (with protein)               :: 3,597
            Genes (RNA)                       :: 51
            rRNAs                             :: 4, 5, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 1 (5S, 16S)
            partial rRNAs                     :: 4, 4 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 78
            CDSs (without protein)            :: 78
            Pseudo Genes (ambiguous residues) :: 0 of 78
            Pseudo Genes (frameshifted)       :: 30 of 78
            Pseudo Genes (incomplete)         :: 56 of 78
            Pseudo Genes (internal stop)      :: 22 of 78
            Pseudo Genes (multiple problems)  :: 26 of 78
            CRISPR Arrays                     :: 6
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Clostridioides difficile"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN17377984-rid13774423.guided.0254"
                     /isolate="8b495a7c-6334-4cb4-a619-a14dbf286c5a"
                     /isolation_source="Farm sample"
                     /host="Sus scrofa"
                     /db_xref="taxon:1496"
                     /country="Ireland"
                     /collection_date="2015-05-01"
                     /collected_by="ModMedMicOxford"
     gene            complement(509..1276)
                     /locus_tag="L3M60_003725"
     CDS             complement(509..1276)
                     /locus_tag="L3M60_003725"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009897376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBN6228307.1"
                     /translation="MKRKVQVKNITIGEGRPKICVPIIGKNKEDIIKEAKELKGVCLD
                     IIEWRVDFFENVENIKEVKEVLYELRSYISDIPLLFTFRSLAEGGEKVISKDYYTTLN
                     KEISSTGLVDLIDVELFMGDEVIDEVINFAHKKEVKVIISNHDFNKTPKKEEIVSRLC
                     RMQELGADLPKIAVMPQDEKDVLVLLEATNEMFKIYADRPIITMSMSGMGVISRLCGE
                     IFGSALTFGAAKSVSAPGQISFKELSSVLNLLHKSIN"
     gene            2037..2669
                     /locus_tag="L3M60_003726"
     CDS             2037..2669
                     /locus_tag="L3M60_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003433837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HBN6228308.1"
                     /translation="MTIPNSTKVYPRNNDYQTVYLKNVITRENIEVGDYTIYNDFCND
                     PRDFEKNNVLYHYPINNDKLIIGKFCSIACNAKFLMTSGNHTLKSKSTYTFPIFYEEW
                     GLDINHITDAWDNKGNIIIGNDVWIGYDSIIMSGVHIGDGAIIGTRAVVTNDVPPYSI
                     VGGVPAKIIKKRFNNNTISKLLEIKWWDWPYKKIQSNIKHIQSGNIDELV"
BASE COUNT         1040 a          376 c          285 g          997 t
ORIGIN      
        1 gtattgaaat ttccaaaatc ttggacaaag aatatgcaaa aatattggct aagtgaaaat
       61 aagtatccca tacattctac tgtaattgat gatgatagat ataaatgttg taatgtacaa
      121 tatacaaaga atagagtgat aatattatat tacatatata acctttaata tacataatta
      181 caaaaaagta attacaaata cacataatac aaatatatct tctactttag ataaaacagt
      241 tattataaaa acttactatc tataaataat agaaatgcta gtataccatc tataatccaa
      301 tgtctagtag gttttaatgg catattttaa atatgccata gttgatttat aagattttta
      361 taccactttc aaaatacaca ctaaatagtt gcttttcaga tattacactt aattaaccaa
      421 tttaaatttt actatctgaa aagtttttta tatttaattt atatatcttc aaaaataact
      481 cctaatattt ctatataatt atattaaatt aattaatact cttatgtaat aaattaagta
      541 ctgagcttaa ctctttaaat gatatttgac ctggtgctga aacacttttt gcagctccaa
      601 aagttaatgc agaaccaaat atttccccac acaatctgct tataactccc attcccgaca
      661 ttgacattgt tattattggt ctatctgcat aaattttaaa catttcattt gtagcttcaa
      721 gtagaactaa cacatctttt tcatcttgtg gcataacagc tattttgggt aaatctgcgc
      781 ctaattcctg cattctacat aaacgagata caatttcttc tttttttgga gttttattaa
      841 agtcatgatt agatataatt actttcactt cttttttatg agcaaaatta attacttcat
      901 ctataacctc atctcccata aataactcta catctattaa atctactaaa cctgtactag
      961 aaatttcttt atttaaagta gtataataat cttttgatat tactttttct cccccttctg
     1021 ctaaacttct aaatgtgaat aataaaggga tatcagatat ataacttctt aattcataga
     1081 gaacttcctt aacttcttta atattttcta cattctcaaa aaaatcaact cgccactcta
     1141 taatatccaa acatactcct tttaattctt tagcctcttt tataatatct tctttatttt
     1201 taccgataat cggtacacaa attttaggtc taccttctcc aatagtaata ttttttactt
     1261 gaacttttct tttcatattc tcctccataa tcagaaattc tatatgattt taaattctat
     1321 tttctgcata atttaattat acattataaa tgctacacat tataaaaata ctaatttaaa
     1381 aataattttt attctaattc ttaaaacaat cccacatatt ttagaaaaga tactctataa
     1441 tataaaaaca cttctttctt atctaaattt attttaacat tctataattt tcataacatt
     1501 acttaagttt tatctatata atcaatatta ttctggatga taatagaatt aatatataaa
     1561 ttatagtata attatataga aataactcga attttactac ttatgaagaa atttgatatc
     1621 aataaattca aatcatttgc attacttcag agttttggct aaagttaaac accatactaa
     1681 acccaaaata gattttacag ctatacttca agagtaattg attgtaatag taccatataa
     1741 tcatccttta gcattaagtg acagtgtaga ttaaaaggat aacaatgtta atgttcttta
     1801 aattaaaaat tattttgtat ataaaatata ttaaattatt gcaagcttta ttgttttgat
     1861 atacaatcaa ctaagcatat agcctttata aaatactaaa tagttaaaag tctgttttta
     1921 aaaacaaaat gtataaatta aaatatagat actatcaagg agtgattctg tgaagagatg
     1981 tattgtaata gtggaatagt caaaaacata atatatatca aaggagagat ttaaatatga
     2041 ctattccaaa ttcaactaag gtatatccaa gaaataatga ttatcaaact gtatatctta
     2101 aaaatgtaat aacaagagaa aatatagaag ttggagatta cacaatatat aatgactttt
     2161 gtaatgaccc aagagatttt gagaaaaaca acgtattata tcattaccct attaacaatg
     2221 ataaattaat aattggtaag ttttgctcaa tagcatgcaa tgcaaaattt ctaatgacta
     2281 gtggaaatca cacactgaaa tcaaaatcca catatacctt tcctatcttt tatgaagaat
     2341 ggggactcga tatcaatcat ataacggatg cttgggacaa taaaggtaat attataattg
     2401 gaaatgatgt gtggataggc tatgattcta taattatgtc aggtgtacat attggcgatg
     2461 gtgcaattat tggaacaaga gcagttgtta ctaatgatgt cccaccatat tctattgttg
     2521 gaggagtacc tgcaaagatt ataaaaaaaa gatttaataa taatacaatt tcaaaattat
     2581 tggaaattaa atggtgggat tggccatata aaaaaatcca atcaaacatt aagcatattc
     2641 aatcaggaaa tatagatgaa ttagtttaat cttttataat ttatatatat taaatttt
//
